LOCUS       DAOSRB010000001        42787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1006_length_42787_cov_6.275180, whole genome shotgun sequence.
ACCESSION   DAOSRB010000001 DAOSRB010000000
VERSION     DAOSRB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 42787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42787
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1006_length_42787_cov_6.275180"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(601..1689)
                     /locus_tag="PLZ74_00005"
     CDS             complement(601..1689)
                     /locus_tag="PLZ74_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96736.1"
                     /translation="MGDRGFDWRRRIGWGIVVAGVACVCGVGCLTEKTHAPETTAGHE
                     LKGMSEEDRIARLRQAGFVCERVSGDMADGWRVDLGGAYKKRVATRDLRPLAGLRVVE
                     LGAQYSEVEDLSPLRAMPLKKLDIRSSRVSDLSALKGLAITNLNLHRCCPVADLSPLQ
                     GMPLVDLEIGQTSVRSLEPLRGMPLNRLHMEVTDIPDLSPLKGMPLARLFINYTTVTN
                     LSDVVGLSLKELHMGWTRITDLSPLVGMPLERLHIPGTKVCDLRPLKGSALRFLYMSG
                     TPVTDVEPLRGLPLYFINMTDSAVSDLTPLADLQLRQFLFTPRRIKKGIEAVRAMPTL
                     RIIGVGFSRGTMWSPSEFWAKYDAGEFE"
     gene            complement(1708..2688)
                     /gene="hemB"
                     /locus_tag="PLZ74_00010"
     CDS             complement(1708..2688)
                     /gene="hemB"
                     /locus_tag="PLZ74_00010"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOR96737.1"
                     /translation="MRFPEVRLRRLRRTPAIRRLFDDPVPPASSLIWPVFVVEGHNRC
                     EPIDAMPGQNRMSVDHLLRALEPVAAQGVGGVLLFGQTEQDKDEGGSGAFDERGVVQQ
                     AVPAIRRAYPELVVMTDVCLCAYTAHGHCGPLAPDGSVDNDAANGLLARVAVSHAAAG
                     ADVVAPSAMMDGQVAVIRAALEENGFDQTLLMAYSTKFASSLYGPFREAERSSPSSGD
                     RQGYQASGGNLRAALRESDFDEDEGADILMVKPALFYLDVLSRLRERTDLPLAAYNVS
                     GEYSMLIAAAERGWGDRDAMARESLTAIRRAGAEIILTYWAPRYRELFKL"
     gene            complement(2702..3316)
                     /locus_tag="PLZ74_00015"
     CDS             complement(2702..3316)
                     /locus_tag="PLZ74_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOR96738.1"
                     /translation="MRRRERVLLLLAAGLGCAAGCQAPMFEGTMFEAQPRQDPTVARI
                     ATSTQQMNLEFRQLAEQVAAVSRNQEVLDARLTRLEGQLAALQRSGEEMGALRQELQR
                     VRVEREGLRKEITDDLAGRIETIASRQQAEIRAARAAAERAAPSASASPARSGSGYEH
                     KVERGQTLSQIARGYGKSMESIMKANKITNPSAIRAGQILFIPD"
     gene            complement(3337..3666)
                     /locus_tag="PLZ74_00020"
     CDS             complement(3337..3666)
                     /locus_tag="PLZ74_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96739.1"
                     /translation="MPPATGRRVLKQGPNSVFVDADVRRTPASWRLDVREDAAGNSFF
                     FGKRRRPALRSRGRNRLFHYIAAGGLRQLRRTSADDDREARRRAFLVFLALLSAVWLL
                     FYFLPCL"
     gene            complement(3686..>3997)
                     /locus_tag="PLZ74_00025"
     CDS             complement(3686..>3997)
                     /locus_tag="PLZ74_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOR96740.1"
                     /translation="LASGSGDSAPAAQTVAPPPPIDVTPDKDALSALSLFGQDGRKEK
                     RTRKAGAHRTYVVQPGDTLFRVAERFYGDSTQWKRIRDANRGRIDADGRIRAGQIIEV
                     P"
     assembly_gap    3998..4097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4098..4983)
                     /locus_tag="PLZ74_00030"
     CDS             complement(<4098..4983)
                     /locus_tag="PLZ74_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96741.1"
                     /translation="MVRYGIIVWGSLLLLSGAGCGKSGVQEAESAERGSPLYRKAFAA
                     EQSGDLKEAIRLYNRVLIEEPRSFSAHFQLATLLHDHAEDYVGAIYHYNQYLYLRPEA
                     EKSTLAQDRIRIAEQLLAPQILRKVGDSVQGISQAHLLKENDRLNRLITTLEGEKSML
                     VESKTAAEQERTRLQAENERLRDLLRKMRVEEVAAPEAAAGQAAQAARDAADTRESKR
                     TTDARTLRALREEAAELAAQSEKPQPIRKPVVDAPTTESVLRKVQSRLTGGSDDSVPA
                     ARKEQSRLASGSGDSAPAA"
     gene            5138..6952
                     /locus_tag="PLZ74_00035"
     CDS             5138..6952
                     /locus_tag="PLZ74_00035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR96742.1"
                     /translation="MTTENTKKLWDSARAVLGGNGCLSVIMPVYGLEKIIGQNIQAVC
                     DLLRDEIPFEVLPVDDGSRDGTAESIRQAASRDPEHVRPVFVQVNAGKGNALKCGFAS
                     SRGSHILLLDGDLDLSPARITTFFEVMAHNRADIVIGSKRHPESVIDYPWHRRLASVI
                     YYTLVRLLVGLPVTDTQTGMKLFRREALGWAFDRMLVKAFAFDLEILSIAHARGFKVA
                     EAPIEMHFGNKVGCLSWSNVRQVMNDTLAIFYRLRILRYYGSVEVAPPASRPMTVSVV
                     IACPGPSPYLTECLEALALQTHRAFEVLVLPDEPLTLPEYPFTLHVLPTGKTRPAEKR
                     NTGLWRAQGEIVAFLDDDAYPVPAWLEHAVKYFTLPDVGGVGGPGVTPPHDPFLAQAS
                     GRVYANRLVSGNYRYRYLGDRVRPRIDDFPSCNLFVRTEVLKAIGGYRTDFWPGEDTL
                     LCADVVFGQGKRIVYDPWVQVFHHRRPLFGPHLRQIGRYALHRGHFAKRFPNTSLRLS
                     YLLPSLFVAGVIAGAILSVFYPPLRIPYIAVLATYAALTLLSSLTLHPGLWFVTWLGV
                     MGTHLVYGVRFAQGLLTRRMPCEAGAFDHPSEKPSGAT"
     gene            6949..8181
                     /locus_tag="PLZ74_00040"
     CDS             6949..8181
                     /locus_tag="PLZ74_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HOR96743.1"
                     /translation="MILPRKKRRNTRQRIPQVALLLESSHEISRGMLRGILEYVHLYG
                     PWALHLNSGGANDQKLPDRAAWRGSGIIARIPNPHVAADIVAARLPTVLIDPVDAYLN
                     PRHPLARCCRVSCDSAAVARCAADYYLANGFTRFAFVGEPSGLNWSLWRQKAFVNRIA
                     EAGFACDVYPPPSEEERATWELDRKRMCRWLAKLPKPLAVFAANDARGRQVLNACLAA
                     DLPVPYEVAVLGVNNDVMICETSLPPLSSVAVDMERAGFAAAELLDQLMRKTLRKQRC
                     VTFAPQKVVARASTRRLQVNDRLVIRALEFIRINAGLNVRVSDVADHVGVTQRWVEKR
                     FAQTLNRSVMDEIQRVRMETVRSLVTKSTLTFSAIAQRCGFTNANHLGVLFRHTFGTT
                     MSDYRHQHAPPTLTARPR"
     gene            complement(8270..10171)
                     /locus_tag="PLZ74_00045"
     CDS             complement(8270..10171)
                     /locus_tag="PLZ74_00045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOR96744.1"
                     /translation="MIIRMCGLVALAVCGAEDPVCVTRQGLPNASRIWRAKTQNGCVF
                     YAGCTTQTPRGQVRNRVPPLVVRAFPGAKGVFPFISWGRAYPDNCSSVIETFRAMSDG
                     PSVSPETSWPNVALTLIDLSDQDIGQTERQVRAALEGLVHRTRQRNPARDMLMLHQPE
                     ASFVAAYRAGRTPDVIRWYEEIAEHYGIPSVNLAKAAARKPEWKGAQEEWDALLRDEL
                     GALFSWCAAQPAQAQPVRHACPSPLTPALWNRATLVNYERGDLDPAGWLGWQWSPVDA
                     IFHVAVCEKPGPVMTLYFTGTAAGLYGVTGPDSGDLEVAIDDGPWQRLAVFDPQARDN
                     AYHLFHRMFADELPDTRHAVRIRVAEAVPSGSTGRVARIGWLTVNGRDASPMGSLSPV
                     ALADTLFLQLEPIAYRPPPGRWSRIPKTMERLRSGGTVRMVMLGDSIINNIQSSHFER
                     LIERAYPGSRIEKIVSVRGSTGCWYYQEPEHLQNYVLRHKPDLLVIGGISQKGDVEAI
                     RSVIRQTRAALPEVEVVVMTDVYGGPWAKDLDPYDPKNAADPDPAGTGYRDRLFKMAG
                     EERVEYVNITQPWMRHLTASKQPLGAFKSDAVHANARGSQLIGRFLELYFAPDGWDAY
                     GTAKRALKP"
     gene            10410..11915
                     /locus_tag="PLZ74_00050"
     CDS             10410..11915
                     /locus_tag="PLZ74_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR96745.1"
                     /translation="MNEPSLTLLAIADLHFTGLARQTAQPPQLRGELAQVLLRKVFAR
                     LTHMGVKPDLTVLLGDLTEDGTDPQAKLDLISLNGELTRSGIPVLALPGNHDIAPAAF
                     HRLLGCEPGLHLFGGYGLVLFDDVYGEDRTCARTEDALAMLRQIAADHPDLPLIALQH
                     PPIYPAIDSHYPYRPTNAAEILDTFEKSKVVLSLSGHYHKGQKARMHEGVLYHTVPSL
                     SEAPFRFSLIRLTGHRVEIEEIQLAMGLPNLIDVHCHTEYAYCGTTVNTASCIALSQA
                     LGVTGLCITEHAFQLYFDKKEAMGFAWQRNPEMAEAVWATPERKRMAAYKRFAQTLRS
                     PFVKVGLEVDLYDHGKLLLAPEDAESEWDLLVGAIHLIEDYVPGRTSQKEAERLFLRD
                     VELLTGHPIQVLAHPFRFFSRNRLACPTHLYAAVADLLADTGVAAEVNFHTHQPDPDF
                     LRICVEKGVRLALASDTHDLAEAGEFWPHVQVLKQAGVTPRSFPETLFRFT"
     gene            complement(11929..13731)
                     /gene="ptsP"
                     /locus_tag="PLZ74_00055"
     CDS             complement(11929..13731)
                     /gene="ptsP"
                     /locus_tag="PLZ74_00055"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOR96746.1"
                     /translation="MSNDSDHLTPSRNEARVLTGLAASRGFVAGPAFLFRTTTTEQVP
                     EYGIRPEQVPHELARLADAFTLTRAQIRSLALELSKRIRGDEATLFDGHLMILDDPSF
                     IGSCKEKITTKQVNAESAVNSVAERYASIFAAMDDAYLKERSQDIGDIAKRILRNLLG
                     GADPLAVRVEKPCIVVADELSPSETISLPRNLILGFATDRGSNTSHVSLLARALGIPA
                     VVGLGSISEQVRTGDALLLDGTSGKVILNPGQQERLAFEKMVARSRTLDEKIEEGKCL
                     PGATSDAHRIPMLANIDPGTPLTDLYAVGAEGVGLYRSEYLWLTYDQEPTEEEQTRAY
                     TNVARVFPDGQPVTIRVLDLGGDKMPHYTGALYKEANPFLGNRSIRYLLSHPEVFRRQ
                     LRAILRASAHGNVQLMYPMVATIEEVRAANLEVRACMSALRAEGIPFNETIRRGVMIE
                     TPAAALIADALAAECEFLSIGTNDLIQYTLAVDRVNETVARLYQPTHPAVLRLIDLTV
                     CAGRARGIPVSVCGETAADPVMAVLLIGLGVDALSMSPNLIPLVKKVISQVSIEEARK
                     MAAIVRRMTGTSANKIYTYCRNHVLKLAPDLLYL"
     gene            complement(13721..14017)
                     /locus_tag="PLZ74_00060"
     CDS             complement(13721..14017)
                     /locus_tag="PLZ74_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOR96747.1"
                     /translation="MGKKDTDAETREFVLLNKYGMHVRPAGLFAKVASRFNAEVEVEK
                     DGNVVSGKSIMALMTLEAVCGTVLRVTAHGPQRREALDELESLVARKFDIPDEQ"
     gene            complement(14021..14992)
                     /gene="hprK"
                     /locus_tag="PLZ74_00065"
     CDS             complement(14021..14992)
                     /gene="hprK"
                     /locus_tag="PLZ74_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOR96748.1"
                     /translation="MAKSRQSKVRQSKVTVGQFLTSGRDRLNLELVSGARGLERVIVE
                     PVTHRPGLALSGFFGHFARKHIQVVGMAEHAYLSSLPEALRVQRFEKLFAHKIPCMVF
                     TRGKRVFPEVLRVSERDGVAVLRSGMVTRFFIHAATFVLEGLSAPRCKVHGTMLEVAG
                     VGVLIEGAAGLGKSETALGLVKRGHALVADDLTMLRRDSNNHLIGSAMEVTQYYMEIR
                     GIGIIHVPSIFGVAAVRGEKQLDLVVTLKRQGEADAELDRTGTESMRRTFLGITVPQL
                     VIPVAPGRDLVNLVETAAQEHKLRLSGFVAVRELDARIKRHHAGGEG"
     gene            complement(14992..15306)
                     /locus_tag="PLZ74_00070"
     CDS             complement(14992..15306)
                     /locus_tag="PLZ74_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOR96749.1"
                     /translation="MVIEVTVRNKQASPDVKKYAEKRALKLFETFPRVESVRVVIDVQ
                     RNLYEAEFVVQQKGLTAVGVKGHASSARAVIDVAAARVEKQLRKTLSKRIKANVRKAA
                     KV"
     gene            complement(15358..16113)
                     /gene="lptB"
                     /locus_tag="PLZ74_00075"
     CDS             complement(15358..16113)
                     /gene="lptB"
                     /locus_tag="PLZ74_00075"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOR96750.1"
                     /translation="MEDVVENGGTRIPDIQAIELVKQYGSRTVVNGVSLHASCGEIVG
                     LLGPNGAGKTTTFYMVVGLVRPNAGEIVFRGEPITRMPVYRRARLGLGYLAQEASIFR
                     KLTVEENVLAILETLPLSRAERAARAEELLASLSLTKVARQRAYTLSGGERRKLEIAR
                     ALVRKPAILMLDEPFSGVDPLAVHDIQEIVRGLRDKGLGIIVTDHNVRETLSIVDRAY
                     LVYDGQILCEGPSSFLVQDETARRLYLGKHFRM"
     gene            complement(16130..16666)
                     /locus_tag="PLZ74_00080"
     CDS             complement(16130..16666)
                     /locus_tag="PLZ74_00080"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOR96751.1"
                     /translation="MFGIVGWVVVLLICRAAWGEVRTNRAARAASLLAGDSTGAPERE
                     AVITAERIEFDNKEGVILFDEKVLVDDEQFIMRSDRLLVFMEGTNDVQQIMAVGRVSI
                     TNANRSASCDKAVYTKKDGQIVMTGNARLMRQGDEGGEVTGNRITFWLDDERMEVSPG
                     RVVLPPGTFKRMDTKRLP"
     gene            complement(16672..17223)
                     /locus_tag="PLZ74_00085"
     CDS             complement(16672..17223)
                     /locus_tag="PLZ74_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96752.1"
                     /translation="MVWYVNPLRRAARRLLFLWACLLLGRVEAARFVDPETDRWFARH
                     GDDLWRPLAARWDEVKAAMTSPAKKLSIPLEYHVNGRAKALLRADQAQIFPDGMIFAE
                     KVRVEMFSETGKPDGLLTAEGCLFDRTARHGYCRGRVRVEKGGDRLQGVGMYFSTERQ
                     CIKILGDCEIRTHRIRNNFGRLK"
     gene            complement(17207..18028)
                     /gene="kdsA"
                     /locus_tag="PLZ74_00090"
     CDS             complement(17207..18028)
                     /gene="kdsA"
                     /locus_tag="PLZ74_00090"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOR96753.1"
                     /translation="MGRVSRVKAGCVTFGAPELAIIAGPCVIESAEATIGLAAKLVRL
                     ANELEMPLVFKASFDKANRTSGDAYRGPGLIEGLAILREVRDRFRVPVLTDIHEPGQA
                     AAVAEVADILQIPAFLCRQTDLVIAAAETGRVVNVKKGQFLAPEDMVNVIDKITGTGN
                     RRILLTERGASFGYHNLVADMRSLLIMREFGYPVIFDATHSVQRPGGAGKMSGGDGRW
                     APALARAAVATGVDGVFMETHVNPAEALSDKANAIAFRDLKAVWRTLKGIHGLVR"
     gene            complement(18040..18789)
                     /gene="kdsB"
                     /locus_tag="PLZ74_00095"
     CDS             complement(18040..18789)
                     /gene="kdsB"
                     /locus_tag="PLZ74_00095"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOR96754.1"
                     /translation="MRVIGVIPSRWGSTRFPGKSLHPLCGKPLIQWVVEAATRATLLD
                     EVLVATDDERIARATEGFAAVVMTRPDHPSGTDRVAEAARAAKDDIVVNIQGDEPLID
                     PDLIDALVRRLRDDPGWAMATAASPIRSLTELESRNVVKVVVAHDGGALYFSRLPIPC
                     RRDGEPDLASGLYLHHVGLYAYRGAFLRRLVTEPPCALEQCESLEQLRALYLGGRIAV
                     ILTGEQGLGIDTPADAVAVEAILKERGQAQQ"
     gene            19009..20085
                     /gene="serC"
                     /locus_tag="PLZ74_00100"
     CDS             19009..20085
                     /gene="serC"
                     /locus_tag="PLZ74_00100"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOR96755.1"
                     /translation="MARVYNFSAGPAIMPLEVLQEAQAELVDYKGSGMSIMEMSHRGK
                     EYDAVHSEAAANIRELMGLNDDYAVLFMQGGASFQFAMLPMNFLGEGRTADYVNAGSW
                     GAAAVKQARLIGNVNLAADCQKDIPTRMPDESEMKWTPGAAYSHITTNETISGAQFKT
                     IPETGSPLVADMSSDILSRPLDYSRFAMIYAGAQKNLGPSGVTLVVIRKDFAEQGSIA
                     LPTLLRYQTHIENLSLYNTPPCFSIYMLCLVTRWIKKQGLENLCKVNRDKAQQLYDVI
                     DASGFYRGTAKREYRSDMNVTFRLPTEELETLFIKQAAEAGMKQLKGHRSVGGIRASI
                     YNAFPVAGIEALAAFMKDFEKRNG"
     gene            20157..21929
                     /locus_tag="PLZ74_00105"
     CDS             20157..21929
                     /locus_tag="PLZ74_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOR96756.1"
                     /translation="MRFPYPMLMLLSAALVSRSDDAYPKPGWVEKPDPLASPYAVPGG
                     MLRFAAFQPPKSLNAYIDSNTYTRQVFGLMYETLLATDPVTTEFTPWLACRWTLSDDK
                     RCYTFELDPAARWSDGAPVTAADVKWTFDQIMDPRHATGASKVALGIFDSPELLGPRT
                     VRFRARESHWRNLLALGSFEILPRHALAEQDFNRIDFDRPVVSGPYVLDAVREQIEIR
                     MRRRRDWWADARPATRHTMNFDTLIFRYYSDTGNAFEAFKKGLVDVYAIYAARIWANE
                     TIGEKFDKNWIVKCRVRNHNPIGFQGFAMNMRRPPFDDLRVRQALAHLVDRETMNRTM
                     MFNAYFLHRSYFEDLYDDAHPCTNPVFAFDPPRAKALLREAGYRPNPQTGVLEKDGRP
                     LRFTFLTRDGGTDKFLAVCATTFREAGIEMKIERKDFASWMRDMDEYRFDITWAAWGG
                     SLFRDPESMWHSKEADRPSGNNLTGFKDPQVDALIEKQKTLFSITERNAINREIDARV
                     AAAVPYVLLWNINSTRLLYWNRFGVPDTVLAKFGDERSLLGYWWDDADSAAELKAAMS
                     AKDVLPRRPEWVDFDAIFPSPSSP"
     gene            21980..22783
                     /locus_tag="PLZ74_00110"
     CDS             21980..22783
                     /locus_tag="PLZ74_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR96757.1"
                     /translation="MFPLQLDRPLVVFDIEATGTSPRSDRIIELAAIRLDPDGTETEG
                     YWLLNPGVPIPIETTAIHGITDEHVKDQPEFKDKAQEILAFFGDSDLAGFNAGRFDIP
                     MLSEEFARVGIPFDALSRRLLDAQRIFHTKEPRDLSAALTFYCGRSHADAHGAEADVR
                     ATLDVLRGQFARYDDLPREMEALDKLLNPIDPFNADRSGRLRWIEDEVVINFGKKKGA
                     KLRDLAKDDPGFLKWIIKNDFPQDTRALCENALKGVFPTPPRPKSAPVQ"
     gene            22884..23519
                     /locus_tag="PLZ74_00115"
     CDS             22884..23519
                     /locus_tag="PLZ74_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96758.1"
                     /translation="MKRIIQCSKCEAKLAIFDFGKPINQKCPKCGNAFVVDSEAGKAK
                     APESSAPAPTPAAADKAPTPAPAATSSEPPAAAKPTAPTEAQAPAKTATPDATPDAAP
                     VKPEPAAEPAAKPVDAPAASPDAGQKEITLKKPAPRAAAKPAPAPAPEPDMPELPNSG
                     VSFLHVVVIIGLLVLSIILQVMNKKAAQRSAFDLAERLNRIEAKLRAPQQP"
     gene            23600..23851
                     /locus_tag="PLZ74_00120"
     CDS             23600..23851
                     /locus_tag="PLZ74_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOR96759.1"
                     /translation="MDTNTLEAKLKEMIVGRLFLNVTAEEIDTNASLIDAYGVDSVCL
                     LELVVGLEEAFGIVIEDSDFDVRNFISVAALRDFVQARL"
     gene            complement(23862..24104)
                     /locus_tag="PLZ74_00125"
     CDS             complement(23862..24104)
                     /locus_tag="PLZ74_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96760.1"
                     /translation="MSDTTQQREWGEQPLARILSEAGLTPHDLVTASEVPMTHKMVAR
                     ACKGRWLTPNTRRIVVAALNRATGREYTPSDLFTYG"
     gene            complement(24131..24700)
                     /gene="rfbC"
                     /locus_tag="PLZ74_00130"
     CDS             complement(24131..24700)
                     /gene="rfbC"
                     /locus_tag="PLZ74_00130"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOR96761.1"
                     /translation="MPVRFEPTELDGVILCVPQVFRDARGFFLETYHAAQYREGGVCD
                     TFVQDNRSRSVRGVLRGLHYQLHKPQAKLVSCLRGEIFDVAVDIRRGSPTFGRWSAAV
                     LTEENHHQLYIPAGFAHGFCVLSEGAEIAYKCSEFYDPGDDRGIRWDDPDVRVDWPIQ
                     DPVLSAKDAAQPLLADAELPEWQPAGVVV"
     gene            complement(24700..26316)
                     /gene="pgi"
                     /locus_tag="PLZ74_00135"
     CDS             complement(24700..26316)
                     /gene="pgi"
                     /locus_tag="PLZ74_00135"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOR96762.1"
                     /translation="MNESAWKAVETEVAATRATHLRDWFAKDPKRAERFTVRAAGWTL
                     DYSKNLITPALMRRLVELAEASGLRKEIDAMFSGELINKTENRAVLHTALRNKADCPV
                     YVQGQDVMPKVTAVLDAMSGFAERVRKGKWLGHTGKRIRYVVNLGIGGSDLGPAMACQ
                     ALKPFAKRSLEMHFVSNVDATHLAETLRKVKAEQTLFIVASKTFTTQETMTNAMSARA
                     WLVERLGSETAVAKHFAAVSTDAKRMTEFGIDPENRFGFWDWVGGRYSLPSAVGLPLM
                     IAIGPRLFRKMLKGFHAMDRHFATAPFSRNMPVILGLLGVLYVNGYGAESYAVLPYDQ
                     YLARLAAYLQQLDMESNGKSVDRQGRRVGYSTGPVVWGEPGTNGQHAFYQLIHQGTRL
                     IPCDFIGFCRSHNPLGDHHDKLMANFFAQTEALAFGKTADACRAEGVPETLVPHKTFE
                     GNHPTNTLLADALTPETFGALIALYEHKVFTQGIIWNIFSFDQWGVELGKVLASKVLL
                     ELTASEAPVLRHDASTNTLIARYRAVRGRS"
     gene            complement(26407..28332)
                     /locus_tag="PLZ74_00140"
     CDS             complement(26407..28332)
                     /locus_tag="PLZ74_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOR96763.1"
                     /translation="MGRSLVAVWVLVCGACWAQGGAAQDPLKVTREEVRARLERLPAT
                     HPRLFINRADAFKSIASESGTPSVAGVIRDWIIREADAVLPQKPTVRKQEGFRMQGQS
                     QVQKRVGLLATAYLFTGKAAYVERAKEEMLAAAAFSDWNPKHFLDTATMTLGLALGYD
                     WLYDALDPDTRATVAGAIRDQGLKQALARKYFWTEGNNNWTQVCWSGMVAGALAIHDQ
                     EPELALSIIHKAVTSVPYSIRPYAPKGSYPEGPGYWSYGTTYYVIMLDLLTQALGTDF
                     GLYDLPGFPQTGEYINLVTGPSGRFYNYADGGDGRSHLLALWWLARRSGRFDWLTREV
                     ADLREALRLNNPDKTGLRQIALPALLLLWAREIDEHQLTSRMPLHWSSGGHVPLVVLR
                     TAWADPNAGYLATKGGPASQNHGHMDAGSFIYEADGVRWAVDLGAQRYYSIEKLGMSL
                     WNSKQDSDRWKVFRLNSASHNLITIDGQPHVVGGSGSVAHFSDNPERPHAVLDLSPVY
                     AGQAERVWRACALLPSRTGVIQDRLKGVRAGAAVRWAMVTQATGCQTVGQDMVLTHSG
                     KSLTVRVVHPAEVRWRVIDLAEPPNPWDVANPGARMIAFEVTAPQDGRVETRVVLLPG
                     GTAPVEEVPDLITLSGL"
     gene            28442..30442
                     /locus_tag="PLZ74_00145"
     CDS             28442..30442
                     /locus_tag="PLZ74_00145"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOR96764.1"
                     /translation="MRFSLSHRPATYIPLLCMLTLSSARLLGADETILWLDTLDLSSM
                     TSAGEPPQAGRTVGGNPFKIARKRFERGVGTHAESWLSLEARGGALAFQAWVGVDDEE
                     LIRGKGSVIFRVYADGRRVADSGIRRAREPAFLLRADLTGAQVVTLHVSDAGDGDARD
                     HADWCEAFFTLKQGASLTPLPAPPTEQMGILTPASPLEPLLRGPRRFGVRPGSPLLFR
                     IPATGEKPIRFAARDLPPGVSLDSTTGLLTGAVTRPGSYAITLAAENARGRTERIWHL
                     EVGERLALTPPLGWNAWNVFAHTVSDAKIRQAAEAMVSSGLADHGWAYINIDDYWQTC
                     PGEREDTALMGPGRDPSGRILPNRRFPDMRGLVQYVHGLGLKIGLYSSPGPLTCGGCV
                     GSLGHEARDAQTIAEWGFDYLKYDWCSYAQIAADDTLAEQMRPFLLMGAALRKQPRDI
                     LFSLCQYGIGHVSAWGDKAGGHCWRTTQDITDTWSSISQIAEAQVGLELFAGPGNWND
                     PDMLVVGTVGWGSPRPSRLTPNEQYTHVSLWCLLSAPLLIGCDLTRLDAFTLNLLTND
                     EVLEVNQDPLGRQAVRVQRTDAQEVWAKPLEDGSLAAGIINRGMLTAPVTLDLTTLGL
                     TGPQQLRDLWRQRDLGTFADASFKTTLPGHGVLLLRITPIKQ"
     gene            complement(30505..32490)
                     /locus_tag="PLZ74_00150"
     CDS             complement(30505..32490)
                     /locus_tag="PLZ74_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOR96765.1"
                     /translation="MIEFKKIGVRYGTQSVLEEVSFRVNTGERVGIVGPNGSGKSTLF
                     RLILGEADADAGEVITEGTPRIGHIRQHLAADHEDETLLAYALRGIPGLTEMEEEIHA
                     LQNQLAGAEDEETRNRLLRQLGDVQTEFEHLGGYEMETRVKVSLGGLGFDASEFDKPF
                     KHFSGGWQMRAELSRVLASHPDLLLLDEPSNYLDLPAVEWLQRFLAGYDGTLMLISHD
                     RYLLRTLTSVTVEVDANTATRYNGDLDFYLREREARHANLLAAKANQDRRIEQLERFV
                     DRFKAQASKAAQAQSRMKMIEKIEAVAVKVPKRSLAAGYLRLAPAPHSGAEVARLEHV
                     DFAYDPGRPVLRDVNLSIARGDKIAIVGYNGMGKTTLLRILAGLRVPTAGVCVLGHKV
                     QPGYLSQDFSETVPPDVSVYECAKRCAPDTPEKVIRAQLGAFGFSADDIGKLAGVLSG
                     GEKIRLAFLRLFLAPPNLMLLDEPTTHLDLEGRATLEKALRAYNGTVCLVSHDVEFVR
                     NTVTSVIEITSAGVRRFPGGYTYYREKIATESRNTDNVSRSVPVEQATTDEPVTHARD
                     ARRARAQARSKYQAAIRTLKERVARTEARITDLEAELETLSACLFNPTPETDFAATNK
                     RLKRVQDQLSVFSEEWERDATELDRLQREQQAAVSGT"
     gene            complement(32554..36786)
                     /locus_tag="PLZ74_00155"
     CDS             complement(32554..36786)
                     /locus_tag="PLZ74_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOR96766.1"
                     /translation="MTRFTKNFSACWRTANLIAVAGVVLTTVAELEPLPNPPEFARRA
                     TLSETMLHNLGALRNWEAAHDARAAAPEGLELGVWHVLGPLKPDSPLTDKIINEYGLI
                     DLTVPLEDRHGRHAWIPCPQFKDHAVCDLTAFRNAGPGDRFIAIRPVTLAERCDLKDV
                     MLDVSLSDGYSQWHPGRNRRDEQGTWPVLDDGYALTIRPGPNQFMMVIRPDRDGYRRF
                     YFRVFRRNTQSPASHSWQRSQRRLALMRRALAEVDAAPHQIRMAEWEALASVWVPQEY
                     NRYRYAWVPGCEAEFASNRFATACNDVLAEIAGRLARMEGLPAQALTPYREMLETWVG
                     QARKPEAAWRRSDPRAAFLKSCALRELMTSAATVISIRLAVEDHIRMFKDAYPQGHAA
                     RRQIDARSREIAEELRAFPHSGPDLPERAARQSVALDALRQTVLLDTPLLAFNRVLLA
                     RGSVSFNSNWGGANQIGDELAILSPVAPSGSVTRVHKGTVSDFDLHWDGRRVLFSDRK
                     ILWELDLQAPDDPPRRITDAPPDVWHYDGCYLPDGGLLAVCNACYQAVPCTGEANVGN
                     LHRMDADGRNERRIAFDQDHNWNPTVMHDGRVLYTRWEYADIPHYFSRFLMRMNPDGS
                     GQMEYYGSGSYWPNAMYWPRPIPGDPHRVVCIVSGHHGVSRAGEVILLDPRQGRHEAS
                     GVVQRLPGYGQTVEPVIKDQLVSDVWPRYAAPYPLAEGPAALGAGRYFLVCRQRNAFS
                     GWDLCLIDIFDNVTPIKHFETLPNGQFVNYMSPVPLRARPQPPVIPSQCNPSRKDAVI
                     YLSDIYSGQGLRGYPRGSIKALRVGTHHYRYFGNGFTYAHAFEGGWDVKRILGTVPVH
                     EDGSAFFRVPANTPIFVQPLDAEGKAQQQMRSWYTAMPGETAACIGCHEPQNSVPPVQ
                     PNLAARGKPVAITPWQGPVRGFSFEHEIQPILSRRCVGCHQDKPLKSGSGGQTTMEDF
                     RDKRLQPHDPSLIPPKCEWWWWLGSANKHLKEPPLCFSPAYMRLQKYVRRAGLEADLH
                     LLAPAEFEADTSELVQRLKKGHHGVTLTQEEWEALYTWIDFNVPYCGTWRESNLPPNE
                     KHVRMRAEYKKRYAGLEDRDEEGVPLPPIPRFEPPSAPVAKTTAAPQVDGWPMNAGQA
                     TAAQRAAGEPELTLDLGGGISMRFALIPAGTFVMGRADGFADEAPRHAVAIDKPFYLG
                     VWEVTCEQYARFNARHESGYIEGRNKDRTTRGTFIGSPRHPVVRISWREAMAFCAWLS
                     RVSGRTCTLPTEAQWEWACRAGSDADFAFGPALANHQANLADEGIRDWNYGRAQTGYQ
                     DGARFSAPVGRYKPNVWGLYDMHGNVAEWTRSAYRPYPCDPSGDEDEAGLKVVRGGSW
                     NDTMAFAGSASRWRYAAWQPVYQVGFRVLVVP"
     gene            complement(36811..38931)
                     /locus_tag="PLZ74_00160"
     CDS             complement(36811..38931)
                     /locus_tag="PLZ74_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR96767.1"
                     /translation="MCRGGVAGLCFLLGVCGALAANVTLHVSPQGKDTWSGTLAAPNA
                     AKTDGPLASLGGARDALRRLRDAGTLTPKQAVTVRFQIGRYEQRTPVVFTKEDSGSPE
                     APVVFEAAPNGQVRITGGVALWKMNTLTDTNLLRRLPEAARGKVQVISLPAQGVYEYG
                     VEPVGSSAGPVSGIQLIYGDEVMRLARWPNEGFVTIADTPDGKQGNTFTYAEENPSRW
                     IDEIDPRGQGYWAHDWAACAIAFERIDPATKTITQKVPGSHYGFRKGGHWFGYNLLCE
                     LDKPGEYYVDRLDGKLYFWPPGKSANTRAEVSVGTRLIQLDDVAHMQFRGLTFENCRG
                     EAILISQGESVNVIGCTMRNIGHRAVTIRGGKTHRIAGCEMTRLGDGGIQVSGGNERS
                     LERCGHVVDNNHIHDYARFSMTYGAAVQLYGCGIRVSHNLIHNGPHVAVLFSGREQVM
                     EYNEIHSICLSSGEMGAFYAGRDWTLVGNVIHGNYIHDIYNPRPQRNRAIMLDDGAAG
                     ITMTQNLIVRVAEGISLSAVGNVIDNNVFVDCHPAISGWQTYETPESVTPPKGVHAQM
                     PERLFATPVEDPLWKERYPWLLAFRNAVRDKKLRPPETRTRVERNVIWGGPSVWLAHY
                     KKYPRTVHSWLVGENNLAGADPLFIDAAKGDYRLQSASPAFKLGFRPLPIEKMGLYAS
                     PERAVWPVRHEVTQVCTNLTYVKP"
     gene            complement(39175..39657)
                     /gene="fabZ"
                     /locus_tag="PLZ74_00165"
     CDS             complement(39175..39657)
                     /gene="fabZ"
                     /locus_tag="PLZ74_00165"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOR96768.1"
                     /translation="MSNEEKITWQQLVASGKEYPGSIEELLPHRKPFLFVDRLLYFDQ
                     QNYIGEMTYGEDMWFFQGHFPGYPVVPGVILIETMAQCGGAGLVASGLIQNGVFLLVG
                     VDNAKFRRQVRPGDTLRMEVENRRVNKRMAVQHGKAYVGEELAAEADFRCVMRPATDN
                     "
     gene            complement(39723..40967)
                     /gene="fabF"
                     /locus_tag="PLZ74_00170"
     CDS             complement(39723..40967)
                     /gene="fabF"
                     /locus_tag="PLZ74_00170"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOR96769.1"
                     /translation="MSGKTKVVITGLGVISPVGNTVGEMWDNIRNGVSGIARTTRFDP
                     SRVDSKISGEVKQFDITQYVDPKEARRMALFTQYALAAARQAWQDAGLADGGADPERV
                     GVFVGNGIGGKEVDDEAYQVLFNRGPGRLSPMLIPKLIANEAAGNISIALKAQAAVHT
                     LVTACASGTDALGVAMDMIRAGRADVMIAGGTEATVTEYCVGGFCSMKALSTKYNDTP
                     EKACRPFDAGRDGFIMGEGSAILILEGEAHARARGARIYAELAGYGASGDAYHLTAPD
                     PEAAGAIRAIRLALKDAGLAPDEIDYVNAHGTSTPLNDPMETKAVKTVFGDHARKLKM
                     SSTKSMTGHMLGAAGAIEALICTLAIRDGFYPPTINYTTPDPACDLDVVPNQGVNGPI
                     RAALSTSLGFGGHNGVLVLKSV"
     gene            complement(41004..42020)
                     /locus_tag="PLZ74_00175"
     CDS             complement(41004..42020)
                     /locus_tag="PLZ74_00175"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOR96770.1"
                     /translation="MFVHITGTGSYLPTRVVTNAELAEKLDTTDEWIFSHTGIRSRHL
                     GADDETTSSMAIQAGRAALEAAGVRAEELGMIVVATSTSDYQAFPSTACLVQHALGAT
                     QAAAFDLQAACTGFIYALWMVYGLMQVDPRPTLVIGAEMMSRIVDWTDRNSCILFGDA
                     AGAVVLQTSGQSGGIRDAILRADGSGADLLYREGGARQLATGPWMHNTLFMKGRPVFN
                     FAVKVFDEILCGLLERSGHTFADLACVIPHQANARIVEAVARRMKVPLETFHMNVATT
                     GNTSAASVPVALDDAVRSGKLTDGGLVVLIGFGGGLTYGGILLQWHDVAGGVPPQRPH
                     PESA"
     gene            complement(42110..42286)
                     /locus_tag="PLZ74_00180"
     CDS             complement(42110..42286)
                     /locus_tag="PLZ74_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96771.1"
                     /translation="MKNLLFHRGAQLLVDPLVAGFPALALQTFSKLLSHNERSLYQAG
                     FSLSTARFESVSFS"
     gene            42290..42363
                     /locus_tag="PLZ74_00185"
     tRNA            42290..42363
                     /locus_tag="PLZ74_00185"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42322..42324,aa:Cys,seq:gca)
     gene            complement(42537..>42787)
                     /locus_tag="PLZ74_00190"
     CDS             complement(42537..>42787)
                     /locus_tag="PLZ74_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOR96772.1"
                     /translation="EAALIVENVKNGKGAYGYNVATGQYTDLVKDGVLDPAKVTRSAL
                     QNAASIAGLLLITECMVTEIPEKKEAPAPNPGMGGMDM"
BASE COUNT         8208 a        14356 c        12106 g         8017 t
ORIGIN      
        1 gaagacatgg agagcacgaa ggggaatgga ttgtcatgga aaacccttgg tatcctttgt
       61 gtgtgacgaa atcgggttgc gggctccgcc cgcgtcaatg tggacgtcag gcgcgcgggt
      121 gtccctgcgg gcaacggcgg atccgtcgga ccggaggcgt ccctgtcgac tgtcgcacga
      181 tggtcaaatc tcgagcgtcg caggtcgaat gccgcaggtc gcagaacgat gttcacgcga
      241 atgtgaacgg gcacgaggac gaggacgggg aagctatgga ttttcgattc tggctcgtgc
      301 tgctcatgcg gggtctgccc acttctcatg agatgtggac aagggcactg caaaagaggc
      361 gcggcatgaa cgggattctt accttacaca gtgaatgtgg acccttaccc ttacaggatg
      421 ttgggtacat cggacatcta caggatgttg ggtacatcgg acatcgttcg gggatcgttt
      481 ttcgttgccc ttccggatcg ccgccgctta caagatggtg gacacaacgg acgccgttcg
      541 gggagttccc gtaccggacg tggtctaggt gattgaacgg cgggcgtgcg ggagaagaaa
      601 ctattcgaat tcccccgcat cgtatttggc ccagaattcc gacggtgacc acatcgtgcc
      661 acgtgagaat ccgacgccga tgatgcgcag cgtcggcatg gcccgtaccg cctcgatccc
      721 ctttttaatc cgtcgcggcg taaacaagaa ctgccgcaac tgaagatcgg ccaacggtgt
      781 caggtccgac accgcactgt cggtcatgtt aatgaagtac agcggcaaac cgcgaagcgg
      841 ctccacgtcc gtgaccggag tgccggacat atagaggaac cgcagtgctg accctttcag
      901 gggacgcaaa tcgcacacct tcgtcccagg aatatggagc cgttccaacg gcataccgac
      961 caagggagag aggtcggtga tccgcgtcca ccccatgtgc aactctttta aagacaaccc
     1021 caccacatcc gatagattcg tgacggtggt atagttgatg aacaaccgcg caagcggcat
     1081 tcccttgagc ggggagagat ccggaatatc cgtgacctcc atatgcagtc tgtttaacgg
     1141 catcccccgc aaaggctcca gacttctgac cgacgtctga cctatctcca aatcgaccag
     1201 cggcatcccc tgcaaaggcg aaaggtcggc aaccggacaa catcggtgaa gatttaaatt
     1261 cgtgatggcc aaccccttca gcgcagagag gtcggacaca cgggaactcc ggatatccaa
     1321 tttcttgagc ggcatcgccc ggagcggcga caaatcttcc acttcagagt attgcgcgcc
     1381 cagctcgacg acgcgcaatc cagcgagcgg acgcagatca cgcgtcgcca cccgtttttt
     1441 gtacgcgccg ccgagatcga cccgccaacc atcggccata tcgccggaaa cccgttcaca
     1501 gacaaatccg gcctgccgta accgcgcgat ccggtcctcc tcggacatcc ccttcagttc
     1561 atgtcccgca gtcgtctcgg gcgcatgcgt tttctccgtc aggcatccca cgccacagac
     1621 acatgcgaca cctgcaacga cgatacccca tcctatgcgc cgccgccaat caaatccccg
     1681 atcccccatc ccgtgacctc cgccatccta cagtttgaag agttcgcggt aacgcggtgc
     1741 ccagtaggtg aggatgatct ccgcgcccgc gcgccgaatg gcggtcagcg attcccgcgc
     1801 catcgcgtcc cgatcgcccc accccctctc agcagcggcg atcagcatgc tgtattcgcc
     1861 actgacgttg taagccgcga gcggcaggtc ggtccgctcg cgtagccggg acagaacatc
     1921 cagataaaac aaggcgggct tgaccatcag gatatccgcc ccctcgtctt catcgaaatc
     1981 cgattcgcgc agcgccgcgc gcagattacc gccggaggcc tgatagccct gccggtcacc
     2041 cgaagacgga gaggagcgtt ccgcctcgcg gaacggcccg tacagagacg acgcgaattt
     2101 cgtcgagtac gccatcagca gggtctggtc gaacccgttc tcctccagag ccgcccggat
     2161 caccgcgacc tggccgtcca tcatggcgct gggcgccacc acatccgcac cggccgcggc
     2221 atgcgaaacc gccacacggg cgagcagacc gttcgcggca tcattgtcca ccgatccgtc
     2281 cggcgcgagc ggtccgcaat gaccatgcgc ggtataggcg cacaggcaga cgtccgtcat
     2341 caccaccagt tcgggatagg cgcgccggat cgccggaacc gcctgctgca ccacgccccg
     2401 ctcatcaaac gcaccgctgc cgccctcgtc tttgtcctgc tcggtctgcc cgaacagcaa
     2461 caccccgccc accccttgcg ccgccacggg ttcgagcgca cgcaacagat gatcgacact
     2521 catgcggttc tgtcccggca tcgcgtcgat cggctcacac cggttatgtc cctccaccac
     2581 aaacaccggc caaatcaggg atgacgcggg cggcacggga tcgtcgaaca gcctacgaat
     2641 cgccggggta cgccgcaagc ggcgcaggcg gacttcggga aaacgcatgc ggaccttcct
     2701 ttcaatccgg aatgaacagt atctggcccg cacggattgc ggacggattg gtgatcttgt
     2761 tggccttcat gatcgactcc atgctctttc cgtatccgcg ggcgatttgg gagagggtct
     2821 gccctcgttc cactttgtgc tcatacccgg aaccgcttcg ggcaggtgaa gcggaggcag
     2881 agggtgccgc gcgttccgcg gcagcacggg ccgcccggat ttccgcctgc tggcgtgaag
     2941 cgatcgtctc aatacgtccg gccagatcgt cggtgatctc tttgcgcaac ccctcgcgct
     3001 cgacgcggac tcgctgcaac tcctgccgca gcgcgcccat ctcctcgccg gaacgctgca
     3061 gggccgcaag ctgcccttcc agccgcgtca gcctcgcatc aagcacttcc tgattgcggc
     3121 tgaccgcagc cacctgttcc gcaagctgcc ggaactccag attcatctgc tgcgtgctcg
     3181 tggcgatccg cgccaccgtt ggatcctgac gcggctgcgc ctcgaacatc gtcccttcaa
     3241 acatcggggc ctgacatcct gccgcacatc cgagtcccgc tgccaacagc agcaacacac
     3301 gttctcttct tcgcatccct cctcctttcc cgtccgtcac aggcagggca agaaataaaa
     3361 caacagccag accgcagaaa gaagcgcgag gaacacgaga aacgcgcggc ggcgcgcctc
     3421 ccggtcatcg tccgccgaag tgcggcgcaa ttgccgcaat ccgcccgccg caatgtaatg
     3481 gaacagccgg ttacggccgc ggctcctgag ggccggacgc cgacgcttac cgaaaaaaaa
     3541 gctgttcccc gccgcatcct cccgcacatc cagacgccac gatgcgggtg tccgcctgac
     3601 atccgcatcg acgaacacac tgttcggacc ctgcttgagc acacgtctac cggtggccgg
     3661 tggcatacct tcccatcccc ctctctcaag gcacttcgat aatctgtccc gcccggatgc
     3721 gtccgtccgc atcaatccgt ccgcggttcg cgtcacgaat ccgtttccac tgggtcgagt
     3781 ccccgtaaaa cctctccgca acccggaaaa gcgtatcccc gggctgcacc acataggtcc
     3841 ggtgcgcccc cgctttccgg gttcttttct ccttgcggcc gtcctgcccg aacagcgaga
     3901 gcgcggacag cgcatccttg tccggcgtca cgtcgatcgg cggcggcggc gcgaccgttt
     3961 gggcggcggg cgccgaatcg cccgatccgc tggcaagnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnggg cggcgggcgc cgaatcgccc gatccgctgg caagccgcga
     4141 ctgctctttg cgggcggcgg gcaccgaatc gtccgatccg ccggtaagcc gcgactgcac
     4201 tttccggagg accgattcgg ttgtcggcgc gtccaccacc ggcttgcgga tcggctgcgg
     4261 cttctccgac tgcgcggcca gttccgccgc ctcctcgcgc agcgcgcgca acgtgcgggc
     4321 atcggtggtc cgcttcgatt ccctcgtgtc cgccgcatca cgagccgctt gggccgcctg
     4381 ccctgcggcc gcttccggcg cggccacctc ctccacacgc atcttgcgca acaagtcacg
     4441 caaccgctcg ttctccgcct gcaagcgcgt ccgttcctgc tctgccgcgg tcttggactc
     4501 aacgagcatg gatttctcac cctccagcgt ggtgatgagc ctgttgaggc ggtcattctc
     4561 cttgagcagg tgcgcctgcg agatcccctg caccgaatcc ccgactttcc gcaggatttg
     4621 gggggccagc agttgttcgg caatgcggat ccggtcttgg gccagcgtgc tcttttcggc
     4681 ttccggcctc agatagagat attgattata gtggtagatc gcccccacat aatcctcggc
     4741 atgatcatga agcagcgtgg ccaactggaa gtgagccgag aaggaacggg gctcttcgat
     4801 caacacccgg ttgtaaagcc ggatcgcctc cttcagatcg cccgactgct cggcggcgaa
     4861 ggcctttcga tagagaggcg acccgcgttc cgcactctcg gcctcctgca cgccagactt
     4921 gccgcacccc gcccctgaaa ggagcaggag cgacccccat acgataatcc cgtatcgaac
     4981 cattacaaaa gcccttccgg aaagagatgc gaccccgcat ttttgatttg gtttctgcgt
     5041 cagaccgaca tatcatgtct gcactttggt ttttaggccg ccgcgcctca aacacagaat
     5101 gcatgcgtga tcgtttattg tactgatctc ctgaaaaatg accactgaaa acacgaaaaa
     5161 actttgggat tcggcccgcg ccgttctggg cgggaatggt tgtctgagcg ttatcatgcc
     5221 ggtttacggc ctggaaaaaa tcatcgggca gaacattcag gctgtctgcg atctgctgcg
     5281 cgacgagatt ccgtttgagg tgctcccggt ggatgacggc agccgggacg gcacggccga
     5341 gtccatccgg caggcggcga gccgggatcc ggagcatgtg cgccccgtct tcgtccaagt
     5401 caatgcgggc aagggcaacg ccctcaaatg cgggttcgcc tcttcccgcg gctcgcacat
     5461 cctgctgctg gacggcgatc tggatttatc gcccgcgcgg atcaccacgt tctttgaggt
     5521 catggcgcat aaccgcgcgg acatcgtgat cggctccaag cgtcatcccg aatccgtgat
     5581 cgactaccct tggcaccgac gcctcgccag cgtgatctat tacacgctgg tgcgcctgct
     5641 ggtgggactg ccggtcacgg atacgcagac cggcatgaaa ctcttccgcc gtgaggccct
     5701 cggctgggct ttcgaccgta tgctggtgaa ggcgttcgcc tttgacttgg agattctctc
     5761 gatcgcccat gcccgcgggt tcaaggtggc tgaagcgccg atcgaaatgc atttcggcaa
     5821 caaagtgggg tgtctctctt ggtccaacgt ccggcaggtg atgaacgaca cgctcgcgat
     5881 tttctaccgc ctgcgaatcc tgcggtatta cggcagtgtc gaggtggcgc cgcccgcctc
     5941 ccgtcccatg acggtctccg tggtcatcgc ctgtccaggc ccaagcccgt atctcacgga
     6001 gtgcctcgaa gcgctggcgt tgcagaccca ccgcgcgttc gaagtgctcg tactccccga
     6061 tgagccgctt acgttgccag agtacccctt cacgctgcac gtgctgccca ccggcaagac
     6121 gcggcccgca gaaaagcgca ataccggcct gtggcgggcg cagggtgaaa tcgtggcgtt
     6181 ccttgatgac gatgcctacc ccgtccccgc ctggctggag catgccgtca aatacttcac
     6241 gctaccggat gtcggcggtg tgggcggacc tggcgtcaca ccaccccacg acccattcct
     6301 ggcccaagcg agtggacgtg tctacgccaa ccgcctggtc tccggaaact accgttaccg
     6361 ctatctcggg gaccgtgtgc ggccgcggat cgacgacttc cccagctgca acctcttcgt
     6421 ccgcacggag gtgctcaagg ccatcggcgg ttaccgcacc gatttctggc ccggggagga
     6481 cacgctcttg tgcgccgacg tcgtgttcgg acaggggaaa cggatcgtgt acgacccgtg
     6541 ggttcaggtg ttccaccacc gtcgtccgct cttcggaccc cacctgcggc aaatcggacg
     6601 ctacgcgctg caccgcggcc attttgccaa gcgtttcccc aacacctcgc tgcgcctaag
     6661 ctatttgctg ccctcgctgt tcgtcgccgg cgtgatcgcg ggtgcgatcc tgtccgtgtt
     6721 ttaccccccg ctgcgtatcc cgtacatcgc ggtcctggcc acctacgcgg cactgaccct
     6781 gctgtcgagt ctgaccctgc atccgggact ctggtttgtc acctggctcg gcgtgatggg
     6841 cacacatctg gtttacggcg tgcgcttcgc ccaaggactg ctgacccgcc ggatgccctg
     6901 cgaagccgga gccttcgacc acccgtctga aaaaccaagc ggggcgacat gattttgcca
     6961 cggaaaaaac gacgcaacac gcggcaacgg atcccgcagg tcgcgctgtt gctggaatcg
     7021 tcgcatgaga tctcgcgcgg catgttgcgc ggcattctcg aatacgttca tctctacggc
     7081 ccttgggctc tgcatctgaa ctccggaggc gccaatgacc agaagttgcc ggatcgcgcg
     7141 gcctggcgcg gctcgggcat catcgcgcgc atccccaatc cccacgtggc cgctgatatc
     7201 gtcgcggcac gcctccccac cgtgttgatc gatccggtcg atgcgtacct caacccccgc
     7261 cacccgctcg cgcgctgctg ccgcgtgagc tgcgattccg ccgccgtggc ccggtgtgcc
     7321 gcggactact atctggccaa cgggttcacg cggttcgcct ttgtgggcga accctctggt
     7381 ctcaactggt ccctgtggcg tcagaaggcg ttcgtgaacc gtatcgcgga ggccggattc
     7441 gcttgcgacg tctatccgcc cccctcggag gaggaacgtg ccacatggga actggaccgc
     7501 aaacgcatgt gccgctggct ggccaaacta cccaaacctt tggccgtgtt cgccgccaat
     7561 gacgcgcgcg gcaggcaggt gctcaatgcc tgtctggccg ccgatcttcc ggtgccgtac
     7621 gaagtggccg tgctgggcgt caacaacgac gtcatgatct gcgaaaccag tctgccgccg
     7681 ctctccagcg tggcggtgga tatggaacgg gccggattcg ccgccgctga actgctggac
     7741 caactcatgc gcaaaaccct gaggaaacag cgttgcgtaa cgttcgcgcc gcagaaggtg
     7801 gtggcgcgcg cctccacccg gcgcttgcag gtgaatgacc gcctcgtgat ccgcgccctt
     7861 gagttcatcc gcatcaacgc gggactgaat gtccgcgtgt cggatgtcgc ggaccacgtg
     7921 ggtgtcacgc agcgttgggt cgagaagcgt ttcgcccaga cgctgaaccg ttcggtgatg
     7981 gatgagattc agcgcgtgcg catggaaacc gtccgctcgc tcgtgaccaa gagcaccctg
     8041 accttttccg cgatcgcgca gcgttgcggg ttcacgaacg ccaaccatct cggcgtactc
     8101 ttccgccaca cgttcggcac caccatgagt gattaccgcc atcagcatgc gccgccgacg
     8161 ctcaccgcgc gaccgcgctg acgcgggtcg tgttctctgg cttcgggttt gggggttcag
     8221 taaccgcacc cgttttcggg cagggacacc tccccgagat gtccgcaagt cagggcttca
     8281 gcgcccgctt ggccgtgccg tacgcatccc aaccgtccgg cgcgaaatac aactcgagga
     8341 agcggccgat gagctgactt ccgcgggcgt tggcgtggac cgcatctgac ttgaaggcgc
     8401 cgaggggttg tttggaagcg gtgaggtggc gcatccacgg ttgcgtgata ttcacgtatt
     8461 cgacgcgttc ctcgccggcc atcttgaaca gacggtcccg atatccggtt cccgccgggt
     8521 ccggatctgc cgcgttcttc ggatcatacg gatccagatc cttggcccac ggcccgccgt
     8581 agacgtcggt catgaccacc acctccacct ccggcaaggc cgcgcgcgtc tggcggatca
     8641 ccgagcggat cgcctccaca tcccccttct ggctgatgcc gccgatgacc agcagatccg
     8701 gcttgtggcg cagcacgtag ttctgcaagt gttccggctc ttgatagtac cagcagcccg
     8761 tgctgccgcg taccgacacg atcttctcga tccggctgcc gggatacgcg cgttcgatca
     8821 gccgctcgaa gtgcgaactc tggatgttgt tgatgatgct gtcgcccagc atgaccatgc
     8881 gcaccgtacc acccgagcgt aatcgctcca tggtcttggg gatccggctc cagcgtcccg
     8941 gcggcggcct gtaggcgatc ggctccaact gaagaaaaag cgtgtccgcc aacgcgaccg
     9001 gcgaaaggga gcccatgggg ctcgcgtccc gcccgttgac ggtgagccag ccgatccgcg
     9061 caacccggcc tgtgctgccg gacggcacgg cttccgccac ccgtatccgc acggcatgcc
     9121 gcgtatccgg aagctcgtcc gcgaacatgc ggtggaaaag atgatacgcg ttgtcgcggg
     9181 cctgcgggtc gaacacggcg agccgctgcc acggaccatc atcgatcgcg acctccagat
     9241 cgcctgaatc cggaccggtc acaccgtaca acccggccgc cgtgccggtg aagtacaggg
     9301 tcatcaccgg ccccggcttc tcgcaaaccg ccacatggaa aatcgcgtcc acggggctcc
     9361 attgccaacc caaccaccct gcgggatcca gatcgccccg ttcgtaattg accagcgtgg
     9421 cccgattcca caatgccggc gtcaacggtg acggacaggc gtgccggacc ggttgcgctt
     9481 gagcgggttg cgccgcgcac caggaaaaga gcgcgccgag ttcgtcgcgc aacagcgcat
     9541 cccactcttc ctgcgcaccc ttccattccg gtttacgcgc cgccgctttc gcgagattca
     9601 cactcggaat cccgtaatgc tccgcgatct cctcatacca gcggatcacg tccggcgtcc
     9661 ggcccgcccg atacgccgcc acaaaagatg cctccggctg atggagcatc aacatatccc
     9721 gggccgggtt ccgctgccgg gtccggtgaa ccagtccctc cagagccgcg cggacctgac
     9781 gctccgtctg tccgatatcc tgatccgaga gatcgatcaa cgttaacgcg acattcggcc
     9841 agctcgtttc cggagacaca gaaggaccgt cggacatagc ccggaacgtt tcaatgaccg
     9901 aggagcaatt gtcgggatag gcgcggcccc atgagatgaa ggggaagaca cctttcgcac
     9961 ccggaaacgc ccggaccacc agcggcggca cgcgattcct gacttgtccc cgcggcgttt
    10021 gggtcgtgca cccggcatag aatacgcatc cgttctgcgt tttggcgcgc cagatgcggc
    10081 tcgcgttcgg caatccctga cgcgtgacgc acaccggatc ttcggcaccg cataccgcca
    10141 gagccacgag cccgcacatc ctgattatca tccttttttt ccgcattgtt tgcctccttt
    10201 tgctctcgtc cacccgatct ggtttccggt tgtgtagcat ggcctccggc gcgtcgcctg
    10261 tcaatcccgg aatccggtct gcaggacgcc ttttgaacgc gcggttttcg tgttgacccc
    10321 gcatgcgact tcacttaccc ttaaacgact tttccgggac cggaaggtca cgttgttttc
    10381 cttcgcgacg tattggattt ctttttccca tgaatgaacc ctctctcacg cttctggcca
    10441 tcgcggatct gcatttcacc ggactggcgc gtcaaaccgc ccaaccgccg caactgcgcg
    10501 gcgagttggc gcaggtgttg ctgagaaaag tgttcgcacg tttgacacac atgggggtca
    10561 agccggacct caccgtgctg ctcggtgatt tgacggaaga cggcacagat cctcaagcga
    10621 agctggacct gatctcgctg aatggcgaac tgacgcgcag cggcatcccg gttctggcac
    10681 tgccgggcaa tcacgacatc gcgccggccg cctttcaccg cctgctcggt tgtgagcccg
    10741 gcctccatct gttcggtgga tacggcttgg tgctttttga tgacgtgtac ggtgaggacc
    10801 gcacctgtgc gcggacggaa gacgcgctcg cgatgctccg gcagatcgcc gccgaccatc
    10861 cggatctgcc gctgatcgcg ttgcagcatc cgccgatcta tccggcgatc gattcacact
    10921 atccgtaccg tcccaccaac gccgccgaaa tcctcgacac gtttgagaag tcgaaggtgg
    10981 tcctctcttt gagcggtcat taccacaaag gccaaaaggc ccgcatgcat gagggtgtgc
    11041 tctaccatac cgtgccctct ctctcggaag cgccgttccg gttcagcctg atccgcctga
    11101 ccggccaccg cgtggaaatc gaggaaatcc aactcgcgat gggcttgccc aaccttatcg
    11161 atgtccattg tcacacggag tacgcctatt gcggcacgac ggtcaacacc gcgtcctgca
    11221 ttgccctctc gcaggcgttg ggcgtgacgg ggctctgcat cacggagcac gccttccaac
    11281 tctatttcga caaaaaagag gcgatggggt tcgcgtggca acgcaacccg gagatggctg
    11341 aagcggtttg ggcgacaccg gagcgcaagc gcatggccgc atacaaacgg ttcgcgcaga
    11401 ccctgcgttc gcccttcgtg aaggtcggcc tcgaagtgga cctctatgac cacggcaagt
    11461 tgctgctcgc cccggaggat gccgaaagcg aatgggacct gctggtcggc gcgattcacc
    11521 tcatcgaaga ttatgtcccc ggacggacca gccagaaaga ggccgagcgt ctcttcctgc
    11581 gcgacgtgga acttctgacc ggacacccga tccaagtgct ggcgcaccct ttccgtttct
    11641 tttcacgcaa ccgtctggca tgccccacgc acctgtatgc ggcggtggct gatctgctgg
    11701 ccgacaccgg cgtggcggcg gaggtgaact tccacacgca tcaaccggac ccggatttcc
    11761 tgcgcatctg cgtcgaaaaa ggtgtgcggc tggcgctggc gtccgacacg cacgatctgg
    11821 ccgaggcggg cgagttctgg ccgcatgtgc aggtgctgaa acaggcgggc gtgaccccga
    11881 ggagctttcc cgaaacgctc ttccgtttca cgtgacacgc ggggcttact acagataaag
    11941 cagatcgggc gcgagtttca acacatggtt gcggcaatag gtgtaaatct tgttcgctga
    12001 cgtgccggtc atgcgccgca cgatcgcggc cattttgcgg gcctcctcaa ttgagacctg
    12061 gctgatgacc ttcttgacca gcgggatcag attcggcgac atgctcaacg cgtccacccc
    12121 caacccgatc agcagcacgg ccatgacggg atccgcggcc gtctcaccgc agaccgacac
    12181 cggaatcccg cgcgcgcggc cggcgcagac ggtcagatcg atcagacgga ggactgccgg
    12241 atgggttggc tggtagagcc gggcaaccgt ctcattcacc cggtccaccg ccaacgtgta
    12301 ctgaatcagg tcattggtgc cgatcgacag aaactcgcac tccgcggcca aggcgtcggc
    12361 gatcagcgcc gctgcggggg tctcaatcat cacgccgcgc cggatcgtct cgttgaaggg
    12421 aatcccctca gcccgcaacg cactcatgca ggcacgcact tcgaggttcg cggcgcgcac
    12481 ctcctcgatg gtcgccacca tgggatacat gagttgcacg ttcccatgcg cgctcgcgcg
    12541 caaaatcgcg cgcaactgcc gccggaagac ctcgggatgg ctgagcagat accggatcga
    12601 gcggttgccg aggaaagggt tggcctcctt atacaacgca cccgtatagt gcggcatctt
    12661 gtcgccgccc aaatccagca cacggatcgt gaccggttgc ccgtcgggga acacgcgggc
    12721 cacattcgtg taggcgcggg tctgctcctc ttcggtcggc tcctgatcgt aggtcagcca
    12781 caggtattcg gaacggtaga gaccgacccc ctcggccccc accgcataga gatcggtaag
    12841 cggcgtgccg ggatcgatat tcgccagcat cggaatccgg tgggcgtcgg aagtggcgcc
    12901 gggcaggcat tttccctcct cgatcttctc gtccagcgtc cgggaacggg ccaccatttt
    12961 ttcaaaagcg aggcgctcct gttggcccgg gttgaggata accttgccgc tggtcccgtc
    13021 cagcagcagc gcatcgccgg tccgcacctg ttcagagatc gaaccgagtc cgaccaccgc
    13081 cgggataccc agcgcacgcg ccaacagcga tacgtgggag gtgttgctgc cgcggtctgt
    13141 cgcaaatccg aggattaagt tccgcggcag cgaaatcgtc tcggacgggg agagttcatc
    13201 cgccaccacg atgcagggct tctccacgcg caccgccaga gggtcggccc cgcccaaaag
    13261 gttccgcaga atccgcttcg cgatgtcccc gatgtcttgg gaacgctcct tgagatacgc
    13321 atcgtccatg gcggcgaaga tggaggcata ccgttccgcc acactgttca ccgccgactc
    13381 cgcgttcacc tgtttcgtgg tgatcttctc cttacaggag ccgatgaaag aagggtcgtc
    13441 gagaatcatc agatgcccgt cgaagagggt ggcctcgtct ccgcgaatcc gtttgctgag
    13501 ttcaagcgcg agcgaacgga tctgcgcccg cgtcaacgtg aacgcatcgg ccaagcgcgc
    13561 cagttcatgg ggcacctgtt cgggacggat cccgtattcc ggcacctgct cggtcgtcgt
    13621 ggttcggaac agaaaagccg gccccgcgac aaagccgcgc gaggcggcca ggccggtcag
    13681 aacacgcgcc tcgttgcgcg agggcgtcag atgatccgaa tcattgctca tcagggatat
    13741 cgaatttgcg ggcaaccagc gactccaact catcgagcgc ctcacgcctc tggggaccgt
    13801 gggccgttac gcgcaggacc gtgccacaga ccgcctccaa cgtcatcaac gccatgatgc
    13861 tcttgccgga cacgacattc ccgtctttct ccacctcaac ctcggcgtta aagcgcgagg
    13921 ccactttggc gaaaagtccg gccggacgaa catgcatgcc gtatttgttc agcaggacaa
    13981 actcccgtgt ctccgcatcg gtatcctttt ttcccacgat ctacccctcc ccaccggcat
    14041 gatgccgttt gatccgcgca tccagttcgc gaaccgcaac aaaccccgaa agccgaagct
    14101 tatgctcctg cgccgcggtc tccaccagat tgaccagatc ccgtcccggt gcgaccggga
    14161 tcaccaactg cggcacggtg attccgagaa acgtccgccg catcgactcc gtacctgtgc
    14221 ggtcgagttc ggcatccgcc tcgccctgcc gcttgagcgt caccaccaga tccagttgtt
    14281 tctcgccgcg caccgccgcc acaccgaaga tcgagggcac atggatgatg ccgataccac
    14341 ggatctccat gtaatactgc gtcacctcca tagcggaccc gatcagatgg ttgttgctgt
    14401 cacgccgcag catcgtcagg tcatcggcca ccagcgcgtg gccccgtttg accagaccca
    14461 gcgcagtctc gcttttgccc aaaccggccg cgccctcgat gagcacgccg accccggcca
    14521 cctccagcat cgtgccgtgc actttacagc gcggcgcaga cagaccctcc agcacgaagg
    14581 tggcggcatg gataaaaaac cgcgtcacca tcccggagcg caacaccgcc accccgtcgc
    14641 gctctgacac gcgcaacact tcgggaaaga cccgtttgcc gcgggtgaac accatgcagg
    14701 ggatcttgtg ggcgaacaat ttttcaaagc gctgcacacg cagcgcctcc ggcagggagg
    14761 agagataggc atgctccgcc atccccacca cctgaatgtg cttccgcgcg aaatgcccga
    14821 aaaaaccgga cagtgccaaa ccggggcgat gggtcacggg ctcgacaatc acccgttcga
    14881 gaccgcgcgc cccggagacc agttccagat tcaaccggtc acggccgctc gtgagaaact
    14941 ggccgaccgt gaccttcgat tgccggacct tgctctgtct gcttttggcc attacacttt
    15001 ggcggctttc cggacattcg ccttaatgcg cttagacaac gttttccgca actgtttctc
    15061 gacacgtgcg gccgcgacat caatcaccgc tctcgcgctg gacgcatgcc ctttgacacc
    15121 gaccgccgtc agccccttct gctgcacgac gaattcggcc tcatacaggt tacgctgcac
    15181 atcgatgacc acacgcacgc tctccacccg cggaaacgtc tcgaacagtt tgagcgcgcg
    15241 cttctcggca tactttttca catccgggga ggcctgcttg tttcggaccg tcacttcaat
    15301 taccataaac cctcttcctt tcctttgtgc ccgccgtcgc gcaggcgttt tgttttgtca
    15361 catcctaaag tgtttgccga gatacaaccg acgcgccgtc tcgtcctgca caaggaacga
    15421 actcgggccc tcacataaaa tctggccatc atacacgagg taggcgcggt ccacaatcga
    15481 gagcgtctcg cgcacgttgt gatccgtcac gataatgccg agccccttgt cgcgcagccc
    15541 tctcacaatc tcctggatat catgcaccgc caacgggtcc accccgctga acggctcgtc
    15601 cagcatcaga atcgcaggct tgcgcaccag cgcgcgcgcg atctcgagct tgcgccgttc
    15661 gccgccactg agcgtgtagg cccgctgccg ggcgaccttg gtgaggctca ggctcgccag
    15721 cagttcctcg gcgcgggccg cacgttcggc gcgcgagaga ggcagggtct ccagaatcgc
    15781 cagcacgttc tcctccaccg tcagcttgcg gaagatggac gcctcctgag cgaggtaccc
    15841 caggcccagg cgcgcgcggc ggtacaccgg catccgcgtg atcggctcgc cgcggaacac
    15901 gatctcgccg gcgttgggcc gtaccagccc caccaccata tagaacgtcg tggtcttgcc
    15961 cgcaccgttc gggccgagca accccacgat ctccccgcaa ctcgcatgca gcgagacgcc
    16021 gttgaccacc gtacgcgaac catactgctt gaccaactcg atggcctgaa tgtccggtat
    16081 gcgcgtccct ccgttttcga caacgtcttc catgtcccgt ctcattccct taggggagcc
    16141 gcttcgtgtc catccgctta aacgtgccgg gcggaagcac cacgcgcccg ggcgagactt
    16201 ccatccgctc gtcatcaagc cagaaggtga tccggttacc cgtgacctcc cccccctcat
    16261 ctccctgccg catcagccgc gcattaccgg tcatcacaat ctgaccgtcc tttttggtgt
    16321 acacggcctt gtcgcaacta gccgaccggt tcgcgttcgt gatgctcacc cgtccgaccg
    16381 ccatgatctg ctgcacatcg ttggtaccct ccatgaacac cagcagccgg tctgaccgca
    16441 tgataaactg ctcgtcatcc accagcacct tctcgtcaaa caggatcacg ccctctttgt
    16501 tatcgaactc gatccgttca gccgtgatca ccgcctcgcg ttccggtgcg ccagtcgaat
    16561 ctcccgccaa caggctcgcg gcgcgtgccg ccctgttcgt ccgaacctca ccccatgcgg
    16621 cccggcagat caacaggacc acgacccagc cgacaatacc aaacatcctt ctcacttgag
    16681 cctcccgaag ttattgcgta tgcgatgtgt ccgaatctcg caatcaccca gaatcttaat
    16741 acattggcgc tcggttgaaa agtacatacc cacaccctgt agccggtcgc cccccttttc
    16801 cacacggacc cgcccccggc aatacccgtg ccgggccgtg cggtcgaaaa ggcacccctc
    16861 ggccgtcaac aagccatccg gtttcccggt ctcgctgaac atctcgacgc gcaccttctc
    16921 ggcaaaaatc atcccgtccg ggaagatttg cgcctgatcg gcgcgcaaga gcgctttggc
    16981 ccgcccgttc acgtgatatt ccagcgggat cgaaagcttc ttcgccggcg aggtcatcgc
    17041 cgccttgacc tcgtcccaac gcgccgcaag cgggcgccaa agatcatcgc cgtgtcgcgc
    17101 aaaccagcgg tccgtctccg gatcaacgaa acgggcggcc tcgacccgtc ccaacaacag
    17161 acaggcccag aggaacagca accgccgcgc cgcgcgccgc aaaggattaa cgtaccagac
    17221 catgtatgcc cttcaatgtg cgccacaccg ctttcaaatc ccggaaagcg atcgcgttcg
    17281 ccttgtccga gagcgcctca gccggattga catgggtctc catgaacacc ccgtccacgc
    17341 ccgtggccac cgccgcgcgc gccagggccg gagcccagcg cccgtccccg ccgctcatct
    17401 tacccgcgcc gccgggacgc tgcacgctgt gcgtggcgtc gaaaatcacc ggataaccga
    17461 attcgcgcat gatcaacaga ctgcgcatgt ccgccaccag gttgtgatac ccgaaactcg
    17521 ccccgcgttc ggtgagaagg atccggcggt tgccggtgcc ggtgatcttg tcgatcacat
    17581 tcaccatatc ctccggtgcg agaaactgcc ctttcttgac gttcaccacc ctgccggtct
    17641 ccgcggccgc gatcaccagg tcggtctgcc gacagagaaa ggccggaatc tgcaagatgt
    17701 ccgccacctc cgccactgcc gccgcctgcc ccggctcgtg aatgtcggtc aggaccggca
    17761 cccggaaccg gtcgcgcacc tcccgcaaaa tcgccaaacc ctcgatcagc cccggccccc
    17821 ggtaggcgtc acccgacgtg cggttcgcct tgtcgaacga ggctttgaag accagcggca
    17881 tctccagttc gttcgcgagg cggaccagtt tcgccgccaa tccgattgtg gcttcagccg
    17941 attcgatcac gcacgggccg gcaatgattg ccagttccgg tgccccgaac gtcacacatc
    18001 ccgccttaac ccgacttacc ctccccatac atccgctcct tactgttgcg cttgtcctcg
    18061 ctccttgaga atcgcctcca ccgccaccgc atccgcgggg gtgtcaatcc ccaaaccctg
    18121 ctccccggtc agaatcaccg cgatgcgccc ccccaggtac agggcacgca actgctccag
    18181 actctcgcat tgctccaagg cgcacggcgg ttccgtgacc agacgccgga gaaacgcgcc
    18241 gcgataggca taaagcccca catgatgaag atagagtccc gaggccaagt ccggttcgcc
    18301 atcgcgccgg cacgggatgg gcaaccgcga aaaatagagt gcgccgccat catgggccac
    18361 caccaccttc accacattcc gcgactccag ttccgtgaga gaccggatcg ggctcgcggc
    18421 cgtcgccatc gcccagccgg ggtcgtcccg cagacggcgc accagcgcgt cgatcagatc
    18481 cgggtcgatg agcggttcgt cgccttggat attcaccaca atgtcgtcct tcgccgcacg
    18541 cgccgcctct gccacccggt ccgtgccgga cggatggtcc ggccgcgtca tcaccaccgc
    18601 ggcgaagccc tccgtcgcgc gcgcgatccg ctcgtcatcc gtggcgacca gcacctcatc
    18661 cagcaacgtc gcacgcgttg ccgcctccac cacccattgg atcagcggtt taccgcaaag
    18721 ggggtgcagg ctttttcccg gaaaccgggt cgatccccac cgcgaaggga ttacaccgat
    18781 cactcgcatc gtttcgactc ctaccacatc gtgaaatgcc ccattctccg tgttcccaga
    18841 gagagagtca agccctccag tccgacaagt ccgtattttg tcgcgcggga acgggacttt
    18901 tgccggattg acaactccgg catgtgaagg catgataatc gccgaattga agcaggagaa
    18961 tgtggacgga tgttcgcatc tcagtgccga aaacggaagg agagagttat ggcgcgcgtg
    19021 tataatttca gtgcgggtcc ggccatcatg ccgctggagg tcttgcagga ggctcaagcc
    19081 gaactggtgg attacaaggg ttccggcatg tcgattatgg aaatgtccca tcggggcaaa
    19141 gagtacgatg ccgtccacag cgaggcggcc gccaacatcc gcgaactgat ggggctgaac
    19201 gacgactacg cggtcctgtt catgcaggga ggcgccagtt tccagttcgc catgctgccg
    19261 atgaactttc tgggcgaagg acgcaccgcc gactacgtga acgccggttc ctggggcgcc
    19321 gcggcagtca agcaagcccg cctgatcggc aatgtgaacc ttgccgccga ttgccagaag
    19381 gacatcccca cccggatgcc tgacgagagc gagatgaagt ggacccccgg cgcggcctac
    19441 agtcacatca caacgaacga gaccatctcc ggggcgcagt tcaagaccat accggaaacc
    19501 ggatcaccgc tcgtggcgga catgtcttcc gacatcctct cccgcccgct tgattacagc
    19561 cgctttgcga tgatctatgc cggagcccag aaaaacctcg gtccctccgg cgtcaccttg
    19621 gtcgtgatcc gcaaggattt tgcggaacag ggcagcatcg cgctgccaac cctgctgcgc
    19681 taccagacgc acatcgagaa cctttcgctg tacaacaccc cgccgtgttt cagtatctac
    19741 atgctctgcc tggtcacccg ctggatcaaa aaacagggac tggagaacct ttgcaaagtg
    19801 aaccgcgaca aggcgcagca gctctacgat gtcatcgatg cctccggctt ctaccgcggc
    19861 accgcgaaac gcgaataccg gtcggacatg aatgtcacct tccgcctgcc caccgaggag
    19921 ctggagacgc ttttcatcaa gcaggcagcg gaagccggca tgaaacagct caaaggccac
    19981 cgttccgtcg gcggaatccg cgcatcgatc tacaacgcct tcccggttgc gggcatcgaa
    20041 gcgctggccg cattcatgaa ggatttcgaa aaacgcaacg gctgagatcc ggacacccta
    20101 ctgacgggcg gcaaggcggg ggaatccccg ccttgccggc acctttgatt ttgaacatgc
    20161 gttttccata ccccatgctg atgctgctct ccgccgcgct cgtttcgcgg tcggatgacg
    20221 cgtatccgaa accggggtgg gtcgaaaagc cggatccgct ggccagcccg tacgcggttc
    20281 cgggcggcat gttgcgcttc gccgccttcc agccccccaa aagcctcaac gcctacatcg
    20341 attccaacac ctacacccgg caggttttcg gcctgatgta tgaaacgctt ctggcaaccg
    20401 atccggtcac caccgaattc accccgtggc tggcctgccg ctggaccctt tccgacgaca
    20461 aacggtgtta caccttcgag ctggatcctg cggcccggtg gagtgacgga gcaccggtca
    20521 ccgcagcgga cgtgaagtgg acctttgacc agatcatgga cccccgccac gccaccggag
    20581 catccaaagt cgcgcttggc atcttcgatt ctccggagct tttaggaccg cgcaccgtgc
    20641 ggttccgcgc gcgcgaaagc cactggcgta atctgctggc cctcggctcg ttcgagatcc
    20701 tgccgcgaca cgccctggcg gaacaggatt tcaaccgcat tgacttcgac cgtcccgtgg
    20761 tttccgggcc gtacgtgctg gacgctgtgc gggagcagat cgagatccgc atgcggcgcc
    20821 ggcgcgactg gtgggcggac gcgcgccccg ccacccgcca cacgatgaat ttcgacacgc
    20881 tcattttccg ttactactcg gacaccggaa acgcctttga agccttcaaa aagggcttgg
    20941 tggacgtgta cgccatctat gcggcgcgta tctgggccaa cgagacgatc ggtgagaagt
    21001 ttgacaagaa ctggattgtc aaatgccgtg tgcgcaacca caaccccatc ggattccagg
    21061 ggttcgccat gaacatgcgt cgtccgcctt tcgatgatct gcgcgtgcgc caggcgctcg
    21121 cccatctggt ggaccgcgag accatgaacc gcaccatgat gttcaacgcc tactttctgc
    21181 accgatcgta cttcgaggac ctgtacgacg acgcccaccc ctgcaccaac cccgtcttcg
    21241 cgttcgaccc gccccgcgcc aaggcactgc tgcgcgaggc cgggtaccgc cccaacccgc
    21301 agacaggcgt tttggagaag gacggacggc cactgcgctt caccttcctc acacgcgacg
    21361 gcggcaccga taaattcctc gccgtctgtg ccacgacctt ccgcgaggcc ggcatcgaga
    21421 tgaaaatcga acgcaaggat ttcgcctctt ggatgcggga catggatgag taccgcttcg
    21481 acatcacctg ggccgcctgg ggcggctccc tcttccgcga cccggagagc atgtggcact
    21541 ccaaagaggc ggatcggcct tccggcaaca atctgaccgg attcaaggac ccgcaggtcg
    21601 acgccctcat cgaaaaacag aaaacccttt tcagcatcac cgagcgtaat gccatcaacc
    21661 gcgagattga cgcgcgcgtg gctgcggcgg tcccgtatgt cctgctttgg aacatcaact
    21721 ccacgcgcct gctgtattgg aaccggttcg gcgtgccgga caccgtgctc gcgaaattcg
    21781 gcgacgaacg gtcgttgctg ggatactggt gggatgacgc ggactccgcc gccgagttga
    21841 aagccgccat gagcgcgaaa gatgtgttgc cccgtcggcc ggagtgggtc gactttgacg
    21901 cgatcttccc gtcgccatct tctccctgac ccgttttatt ccgcaaagaa accgaaaaac
    21961 caacccaaga aagacccgta tgttccccct ccagcttgat cgtcccctcg tcgttttcga
    22021 tatcgaagcc accggaacca gcccccgctc cgaccggatc atcgaattgg ccgctatccg
    22081 tctggatccc gacggcacgg agaccgaagg gtactggctg ctgaatcccg gtgttccgat
    22141 tccgatcgag accacggcga ttcacgggat caccgacgaa cacgtcaaag accagccgga
    22201 attcaaggat aaggcgcagg aaatcctcgc ctttttcggc gattccgatc tggcggggtt
    22261 taatgccggc cgcttcgaca tccccatgtt gtctgaagag ttcgcccggg tcggtatccc
    22321 gttcgatgcc ctttcgcgac gccttctgga cgcgcagcgc attttccaca ccaaggaacc
    22381 gcgtgacctc tcggcagccc tgaccttcta ctgcggacgc tcgcatgcgg acgcgcacgg
    22441 tgccgaagca gatgtgcgcg ccacgctcga cgtcttgcgc ggccagttcg cccgctatga
    22501 cgacctgccg cgcgagatgg aagcgctcga caagctcctg aatccgatcg acccctttaa
    22561 tgcggatcgc tccggacgcc tgcgttggat cgaggatgag gtggtcatca atttcggcaa
    22621 gaagaaaggg gccaaactgc gggatctggc caaagacgac ccggggtttc tcaagtggat
    22681 catcaaaaat gattttccgc aggatacgcg cgctctgtgt gaaaacgcgc tcaagggtgt
    22741 ctttcccacg ccgccacgcc cgaaatccgc gccggtccag taacgtcctc cggcacgcgt
    22801 ccgaagcggc gggctgaatc tccggcttgc ccgaccggac gggaacgaat acactaaacc
    22861 cactacttaa aaggagacct cgaatgaaac gcatcataca gtgctcaaaa tgcgaagcca
    22921 aactcgcaat ctttgatttc gggaaaccga tcaaccagaa atgccccaaa tgcgggaacg
    22981 cctttgtcgt ggattcggag gccggcaaag cgaaggcgcc agagtcttcc gcccctgccc
    23041 cgacccccgc agccgctgac aaagccccga cccccgctcc cgcagccacc tcatccgaac
    23101 ccccggccgc ggctaaacct accgccccga ctgaggcgca ggctcccgcc aaaaccgcga
    23161 ctccggatgc gactccggat gcggctccgg ttaaacccga acctgctgca gaaccggccg
    23221 ccaaaccggt tgacgccccg gcggcttctc ccgacgcggg ccagaaggag atcacgctca
    23281 agaaacccgc tccgcgtgcc gccgccaaac ctgctcccgc acccgcaccg gaacctgaca
    23341 tgcccgaact tccgaacagc ggggtctcat tcctgcatgt ggtcgtgatc atcggactgc
    23401 tggtcctctc aatcatcctg caggtgatga ataaaaaagc ggcgcagcgc tcggcgttcg
    23461 accttgctga acgcctcaac cggatcgagg cgaaactgcg ggcgccacaa cagccttaat
    23521 tggaaacagg gggcacaccg gccgcggacg aacatggttc gctccgcgcg tcggtgaacc
    23581 catggacagg gggggacaag tggacacgaa cacccttgaa gccaaactga aagagatgat
    23641 tgtcgggcgg ctgttcctga acgtcacggc agaggagatc gacacgaacg cctcgctgat
    23701 tgacgcctac ggggttgact ccgtctgtct gcttgaactc gtggtcgggc tggaggaagc
    23761 gttcggcatc gtgatcgaag acagcgactt tgatgtccgc aacttcatct cggtggcagc
    23821 gttgcgcgac ttcgtgcagg cccgtctgta aaaaagagca gtcacccgta cgtgaacagg
    23881 tcggaaggcg tgtactcccg ccctgttgcc cggttgaggg ccgcgaccac aatcctgcgc
    23941 gtgttcggcg tcagccaacg tcctttacag gcgcgcgcga ccattttatg cgtcatcggc
    24001 acctctgacg ccgtgaccag atcatgcggt gtgagtccgg cctctgacaa gatccgggcc
    24061 aagggctgtt caccccactc acgctgttgc gttgtatccg acatgcaaat cttccctccc
    24121 ccctgccggt tcagaccacc actcccgcag gttgccattc cggcagttcg gcatccgcca
    24181 acaggggttg cgccgcgtct ttcgccgaga ggaccggatc ctgaatgggc cagtcgacac
    24241 gcacatccgg atcatcccag cggatacccc ggtcatcgcc cggatcgtag aactccgaac
    24301 atttgtacgc aatctcggca ccctccgaaa gcacgcagaa cccgtgcgca aaccctgccg
    24361 ggatgtagag ctgatgatga ttctcctccg tgagcactgc agctgaccag cgcccgaagg
    24421 tcggcgatcc ccggcggatg tcgaccgcca catcgaagat ctccccccgc agacacgaaa
    24481 ccagtttcgc ctgcggtttg tgaagctgat agtgcaaccc ccgcaacacg ccgcgcaccg
    24541 aacgggaacg gttgtcctgc acgaacgtgt cacagacccc gccctcccga tattgcgccg
    24601 cgtgataggt ttcgaggaaa aatccgcgcg catcgcggaa cacctgcggg acacacagga
    24661 tcaccccgtc caattcggtc ggctcgaaac gtaccggcat cacgacctcc cccgcaccgc
    24721 gcggtagcgg gcgatcagcg tgttcgtcga cgcatcgtgg cgcagcacgg gggcttcgct
    24781 cgcggtcaat tcgagcaaca ctttggaagc caggaccttg cccagttcca ccccccactg
    24841 gtcaaacgag aaaatgttcc aaatgatacc ctgcgtgaag accttgtgct catagagcgc
    24901 gatcaacgcg ccgaacgtct cgggcgtcag cgcgtcagcc agcagcgtgt tggtcggatg
    24961 gttcccctca aaggttttgt gcggcaccaa cgtttccgga accccctcgg cgcggcaggc
    25021 gtccgcggtt ttgccgaacg ccaacgcctc ggtctgcgcg aaaaaattcg ccatcagttt
    25081 gtcatggtgg tcgcccaacg gattgtgcga gcggcagaac ccgatgaaat cgcaggggat
    25141 cagtcgcgtg ccctgatgga tgagttgata gaaggcgtgc tgcccgttgg ttccgggctc
    25201 accccaaacg accgggccgg tcgagtatcc aacccggcgt ccctgacgat ccaccgattt
    25261 tccgttactc tccatgtcga gctgctgcag ataggccgcc agccgggcaa ggtactggtc
    25321 atacggcaac acagcgtaac tctccgcgcc atacccgttc acgtagagca cccccagcaa
    25381 acccagaatc accggcatgt tccgactgaa cggtgccgtc gcgaaatgcc ggtccatcgc
    25441 gtgaaacccc ttgagcatct tccggaaaag acgggggccg atcgcgatca tgagcggtaa
    25501 cccgaccgct gagggtagcg agtagcgtcc ccccacccaa tcccaaaacc cgaacctgtt
    25561 ctccggatcg atgccgaact ccgtcatccg tttcgcatcg gtggaaaccg cggcaaagtg
    25621 tttggccacc gcggtttcac tgccgagccg ttccaccagc cacgcccgcg cgctcatcgc
    25681 gttggtcatg gtctcctgcg tcgtgaacgt cttcgacgcc acgataaaca acgtctgctc
    25741 agccttgacc ttccgcagcg tctccgccaa atgggtcgcg tccacattcg agacaaaatg
    25801 catctccagc gaacgcttgg caaaaggttt cagcgcctgg cacgccatgg ccggacccaa
    25861 atccgacccc ccgatcccga ggttgacgac ataccggatg cgtttaccgg tgtggccgag
    25921 ccacttgccc ttgcgcacgc gttccgcaaa acccgacatg gcgtccaaca ccgccgtcac
    25981 cttgggcatc acatcctgcc cctggacata caccggacag tcagctttgt tgcggagggc
    26041 cgtatgcaac accgcacggt tctcggtttt gttgatcagc tcacccgaga acatggcatc
    26101 gatctctttc cgcaacccac tggcttcggc cagctccacc agacgacgca tcagcgcagg
    26161 cgtaatgagg tttttagaat agtccagcgt ccatccggca gcgcgcaccg tgaaccgttc
    26221 cgcccgtttg ggatccttgg caaaccaatc ccgcagatgc gtcgcgcgcg tcgccgccac
    26281 ctcggtttcc accgctttcc atgctgattc attcatgaaa taagtttact atagcggctc
    26341 cctctccgca atcccgatag cgattgggat gccacaggcc gggtccccat tttttcgtcc
    26401 cgtggctcac agaccggaca gcgtgatcag atccggcacc tcttccaccg gcgcggtccc
    26461 gcccggcaag agcacgaccc gcgtttcaac ccggccgtcc tgaggcgcag tcacctcaaa
    26521 ggcgatcatg cgcgcccccg gattggcgac atcccatgga ttcggcggtt cggcaagatc
    26581 gatcacccgc catctcacct ccgccggatg caccacccgc acggtcaggc ttttgccaga
    26641 gtgcgtcagc accatatcct gtcccactgt ctggcagcct gtggcctgcg tcaccatggc
    26701 ccaacggacc gcggcccctg cacggacccc cttgaggcga tcttgaatca ccccggtacg
    26761 ggagggcagt aacgcgcacg cgcgccaaac ccgttccgct tgcccggcgt agaccgggct
    26821 cagatcaagc accgcgtggg gccgttccgg attgtcgctg aagtgcgcca cgctgcctga
    26881 tccgcccacc acatgcggtt gcccgtcgat cgtgataaga ttgtgactcg cgctgttcag
    26941 cctgaacacc ttccaccgat ccgaatcctg ttttgaattc cacaggctca ttcccaactt
    27001 ctcgatcgaa taatacctct gtgcccccaa gtccaccgcc caacgcaccc catccgcctc
    27061 gtagatgaac gaccccgcat ccatgtggcc gtggttttgt gaggcaggcc cgcctttcgt
    27121 ggccagatat ccggcattgg gatccgccca ggctgtacgc aacaccacaa gcggcacatg
    27181 ccctccactc gaccagtgga gcggcatgcg gctcgtaagc tgatgttcgt cgatttcccg
    27241 tgcccagagc aagagcagcg cgggcaacgc gatctgccgc aacccggtct tgtcgggatt
    27301 gttgagccgg agcgcctccc gcaggtcggc aacctcccgc gtaagccaat cgaaacggcc
    27361 gcttcggcgc gccagccacc acaacgcgag cagatgggat cggccatcac cgccgtccgc
    27421 atagttgtag aaccgcccgc tggggcctgt caccagattg atgtactctc cggtctgcgg
    27481 gaaccccggc aaatcataca gaccgaaatc cgtgccgaga gcctgagtca gcagatccag
    27541 catgatcacg taatacgtcg tgccatagct ccagtatccc ggcccttccg ggtaacttcc
    27601 tttcggggca taaggccgga tggagtaggg aaccgacgtc accgctttat gaatgatcga
    27661 caacgcaagt tcaggctcct gatcgtggat cgcgagcgcc cccgcaacca taccgctcca
    27721 gcacacctga gtccagttgt tgttcccctc cgtccagaaa tacttccgcg ccaacgcttg
    27781 tttcaggcct tgatcccgga tcgctccggc cacggtcgct cgcgtgtccg gatccagcgc
    27841 gtcgtaaagc cagtcgtacc ccagcgccaa gcccaaggtc atggtggccg tgtccagaaa
    27901 atgcttcggg ttccaatcgc tgaaagcggc cgcggccaac atttcctcct tcgcccgctc
    27961 cacgtaagca gccttcccgg tgaagaggta tgcggttgcc aacagcccga cccgtttctg
    28021 cacctgactc tggccctgca tccggaaccc ctcttgcttg cggacggtcg gtttctgcgg
    28081 caagaccgca tcggcttccc ggatgatcca atcacggatc acccctgcca ccgaaggggt
    28141 tccgctctcg ctggcgatcg atttaaacgc gtccgcccga ttgatgaaca gccgcgggtg
    28201 cgtggcggga agccgctcca gacgcgcccg cacctcttcc cgtgtcacct tcaacggatc
    28261 ttgcgcggcc cccccttgtg cccagcaggc cccgcaaacc agcacccata ccgcaaccag
    28321 acttctcccc attttttcct ccttatgtat ggtggaacaa tacaaaaaaa agaccgctcc
    28381 agacaagagc gaactggaaa aacgttctat aataggatac cttctatgaa aggagttcat
    28441 catgcgcttt tctctttccc atcgtcccgc cacctatatc ccgcttcttt gcatgttgac
    28501 gctctcgtcc gcccggctac tgggcgcgga cgagaccatc ctctggctgg atacgcttga
    28561 cctcagttcg atgaccagcg caggggaacc gccccaagcc ggtcgcacag taggcggcaa
    28621 cccgttcaaa atcgcgcgca agcgctttga gcgcggtgtc ggcacacatg ccgaaagctg
    28681 gttgagtctg gaggcgcgcg gcggcgcgct cgcgtttcag gcatgggtcg gtgtcgatga
    28741 cgaggagttg atccgcggca aaggatcggt gattttccgt gtgtacgcgg acggacggcg
    28801 ggttgcggac agtggcatcc ggagggcgcg ggagcccgcc tttttgctcc gcgccgacct
    28861 caccggggcg caggtggtga cgttgcacgt ttccgacgcc ggggacggtg atgcccgcga
    28921 ccacgctgat tggtgcgaag cgtttttcac gctgaagcag ggagcttcgc tgacccccct
    28981 gccggctccg ccaaccgaac agatgggcat cctcacaccg gcgtcccctc ttgaaccgct
    29041 ccttcgcgga ccgcgccgtt tcggtgtccg acccggcagc ccgctcctgt tccgcattcc
    29101 cgccaccggc gagaaaccca tccggttcgc ggcacgcgac ctgccgcccg gcgtgtcgct
    29161 ggattcaaca accggtctgc tcacaggcgc ggtcacgcgc cccggctcgt atgcgattac
    29221 cctggccgcg gaaaacgcgc gcggccgcac ggagcggatc tggcatctgg aggtcggcga
    29281 gcgtctggcc ctcaccccgc ccctgggatg gaacgcatgg aacgttttcg cgcataccgt
    29341 ctcggacgct aaaatccgtc aggcggccga ggccatggtg agcagcggac tcgccgatca
    29401 cggatgggcg tacattaaca tcgacgacta ttggcagacc tgtcccggag aacgagagga
    29461 taccgcgttg atgggacccg gtcgtgatcc ctcggggcgt attctgccca accggcgttt
    29521 tcccgacatg cgcggcctgg tgcagtacgt gcacggactg ggactcaaga tcggactgta
    29581 ctcctcgccg gggccgctga catgcggcgg gtgcgtcggc agtttagggc atgaggcacg
    29641 cgacgcgcaa acgattgccg agtgggggtt cgactatctg aaatacgatt ggtgcagtta
    29701 tgcgcagatc gcggcggatg acacgctggc ggaacagatg cggcctttcc tgctcatggg
    29761 tgccgccctg cgcaaacaac cgcgcgacat cctgtttagt ctctgccaat acgggatcgg
    29821 acacgtctcc gcttgggggg ataaggcagg cggtcattgc tggcgcacca cccaagacat
    29881 cacggatacg tggtcgagta tcagccagat cgcggaagcc caagtcgggc tggagctttt
    29941 tgccgggccg ggaaactgga atgatcccga tatgctggtg gtgggaacgg ttggctgggg
    30001 ctcgccccgt ccttcacgcc tgacacccaa cgagcaatat acgcatgtct cactctggtg
    30061 cctgctcagc gcgccgctgc tgatcggatg cgatctgacg cgcctggacg cttttaccct
    30121 caacctgctg accaatgatg aggttttgga ggtcaatcag gatccgctcg gacgccaagc
    30181 cgtccgtgtg caacgcactg acgcgcaaga ggtctgggcc aaacctctgg aggacggcag
    30241 tctggccgcg ggaatcatca accgcggcat gttgaccgcc cccgtgaccc tggatcttac
    30301 tacgctcggc ctcaccggcc cgcagcaatt acgggatctt tggcgtcaac gtgacctcgg
    30361 cacctttgcc gacgcctcct tcaagaccac cctccccggc cacggcgtgc tgctgttgcg
    30421 gatcacacca atcaagcaat gaccggcatc ggcggggacg cctacggtct cgtgtctccc
    30481 gtcttctgtc tgccgcttcg aagatcacgt gccggaaacc gccgcctgct gttcgcgttg
    30541 caggcggtcg agttccgtcg catcgcgttc ccactcctcg gaaaacacac taagctggtc
    30601 ttgcactcgt tttagtcgtt tgttggtggc ggcgaaatcg gtctcgggtg tgggattgaa
    30661 gagacaggcg gaaagcgttt ccagttccgc ctccaaatcc gtgatgcgcg cctcggtccg
    30721 ggcgacccgt tcttttaacg tgcggatggc ggcctgatac ttgctgcgag cctgcgcccg
    30781 ggcgcgccgc gcgtcgcgcg cgtgcgtaac gggttcgtcg gtcgttgcct gttcgacggg
    30841 aaccgaccgg gaaacgttgt ccgtgttccg actctcggtc gcgatcttct cgcggtaata
    30901 ggtatacccg ccgggaaaac ggcgcacacc ggccgacgtg atttcgatga ccgatgtgac
    30961 cgtgttgcgc acaaattcga cgtcatggct gacaaggcag accgtgccgt tgtaagcgcg
    31021 aagcgccttt tccagtgtgg cgcgcccttc cagatccaag tgcgtggtcg gctcgtcaag
    31081 cagcataaga ttgggcggcg caagaaagag acgcaggaac gcgagccgga ttttttcgcc
    31141 gccgctgagc acgccagcca gctttccgat atcgtcggcc gagaaaccga atgcgcccaa
    31201 ctgcgcccgg atcacctttt ccggcgtatc cggcgcgcac cgcttggcgc actcgtacac
    31261 cgagacgtcc ggcggcaccg tttcggaaaa atcctgcgac aggtaaccgg gttgaacctt
    31321 gtggcccaag acacacacgc cagcggtggg gacacgcaga ccggcgagga ttcgcagcag
    31381 cgtggttttg cccatgccgt tatagccgac aatcgcgatt ttgtcgcccc gcgcaatgct
    31441 gaggttcaca tcccgcaata ccggacggcc gggatcatat gcgaaatcca catgttccaa
    31501 ccgggcgacc tccgcaccgg aatgcggagc gggcgcgagt cgcagatatc ccgcagccag
    31561 actgcgtttg ggcaccttga ccgccaccgc ctcgattttt tcgatcatct tcatccggct
    31621 ttgcgcttgc gcggctttgg aagcctgtgc tttgaagcgg tcgacaaaac gttcgagttg
    31681 ttcgatccgc cgatcctggt tggccttggc ggccagcaga ttagcgtgac gcgcctcacg
    31741 ctcgcgcaga tagaaatcga gatcgccgtt gtatcgcgtg gcggtgttgg catccacctc
    31801 caccgtgacc gaagtcaggg tgcgcaacag ataccggtcg tgcgagatca gcatcagggt
    31861 gccgtcgtac cccgccagaa aacgctgcaa ccactccacc gccggcaggt cgagatagtt
    31921 cgaaggctca tccagcaaca gcaggtcagg atgggaagcc agcacgcgcg aaagttcggc
    31981 gcgcatctgc cagccgccgc tgaagtgctt gaacggttta tcgaactccg acgcatcaaa
    32041 acccagaccg ccgagcgaga ccttgacgcg ggtctccatc tcgtatccgc cgagatgctc
    32101 gaactcggtc tggacgtcac ccagttgacg caacaggcgg ttgcgcgtct cctcgtcctc
    32161 cgctccggcc aactggttct gcagcgcgtg gatctcctct tccatctcgg tcaggccggg
    32221 aatcccgcgc aaggcgtacg ccagcaacgt ttcgtcttcg tgatccgccg cgagatgctg
    32281 ccggatatgc ccgatgcggg gtgtgccctc ggtgatcacc tcaccggcat ccgcgtcggc
    32341 ctcccccaga atcagccgga agagtgtgga tttgcccgat ccgttggggc cgacaatccc
    32401 gacccgttca cccgtattca cgcggaaact cacctcttcc agcacgctct gcgtaccgta
    32461 ccgcacaccg atttttttaa actcaatcac agcacatcca tgatccgcat acgggcgcag
    32521 cccgcgaatg ttccctgcgc actctgattc cgctcagggg acgaccaaca cccggaaccc
    32581 gacctgatac accggttgcc aagccgcgta tcgccagcgc gaggcggaac cggcaaaagc
    32641 catcgtgtcg ttccacgagc cgccgcgtac gacctttagc cccgcttcgt cctcatcccc
    32701 cgaggggtcg cacggatagg gccgatacgc gctgcgcgtc cactcggcga cattgccgtg
    32761 catgtcataa agtccccaga cgttcggctt ataccggcca accggcgcac tgaaccgcgc
    32821 gccgtcttga taccctgtct gcgcccggcc gtagttccag tcgcggatgc cttcatccgc
    32881 cagattggcc tgatggttgg cgagtgcggg tccgaaggca aagtcggcat cgctgccagc
    32941 gcggcaggcc cactcccact gcgcttcggt ggggagagta caagttcgtc cgctgacccg
    33001 cgagagccag gcgcagaacg ccatcgcctc gcgccaagag atgcgcacca cggggtgacg
    33061 cggcgatccg atgaacgtgc cgcgcgtcgt gcggtctttg ttacgcccct cgatgtaccc
    33121 gctctcatgc cgggcattga accgggcata ctgttcacag gtgacctccc aaacgccgag
    33181 atagaacggt ttgtcgatcg ccaccgcatg ccgtggcgcc tcatccgcga acccgtcggc
    33241 acgccccatc acgaacgttc cggcggggat aagtgcgaaa cgcatgctga tccccccgcc
    33301 cagatccaga gttagttccg gttcaccggc ggcccgttgc gcggccgtag cctgtccggc
    33361 attcattggc cagccatcga cctgcggcgc ggccgtcgtt ttcgcgaccg gagcggacgg
    33421 cggctcaaag cgcggaatcg gcggaagcgg aaccccctcc tcatcacggt cttccaatcc
    33481 ggcataccgt tttttgtact cagcgcgcat gcgcacatgt ttctcgttcg gcggcagatt
    33541 ggactcacgc catgtgccgc agtacgggac attgaagtcg atccacgtgt agagggcttc
    33601 ccactcctct tgcgtcaatg taaccccgtg atgccctttc ttaagccgct gcaccaactc
    33661 gctggtgtcg gcttcgaact ctgctggagc gagcaggtgg agatccgcct ccaagccggc
    33721 acggcgcaca tacttctgca agcgcatgta ggcgggactg aagcagaggg gcggttcctt
    33781 gagatgctta ttcgccgaac cgagccacca ccaccactcg catttggggg gaatcagact
    33841 gggatcatgc ggttgcagcc gtttgtcgcg gaaatcctcc atcgttgttt gccccccgct
    33901 cccggacttc agcggcttgt cctgatgaca ccccacgcaa cgccggctga gaatcggctg
    33961 gatctcgtgt tcgaacgaaa aaccgcgaac cggtccctgc cacggggtga tggcaacggg
    34021 tttgccgcgc gcggccagat tgggctgaac cggcggcacg gagttctgcg gctcgtgaca
    34081 tccgatgcag gcggcggtct cacccggcat ggcggtatac caactgcgca tttgctgctg
    34141 cgctttgccc tccgcgtcca gcggctgaac gaagatcggc gtgttggcgg gaacccggaa
    34201 aaaagccgaa ccgtcctcat gcaccggcac ggtgccaaga atgcgcttca catcccagcc
    34261 tccctcaaaa gcgtgggcat aggtgaaccc gttgccaaag tatcgatagt ggtgggtgcc
    34321 gacgcgcagc gctttgatcg atccgcgtgg ataaccgcgc agaccttggc cgctatagat
    34381 gtcggaaaga tagatcactg cgtctttccg ggagggattg cattgcgacg ggatcaccgg
    34441 cggctgcggc cgggcgcgca gcggcaccgg cgacatatag ttgacgaact gcccgttcgg
    34501 cagagtttca aaatgtttga tgggcgtgac attatcgaaa atatcgatca ggcagagatc
    34561 ccagcccgag aaggcattgc gttggcggca caccagaaag tagcgcccgg cacccagcgc
    34621 cgcaggccct tcggccagcg gatacggggc agcgtaacgc ggccagacat cggagacgag
    34681 ctggtccttg atcaccggct ccacggtttg tccgtatccc ggcaagcgtt gcaccacgcc
    34741 agatgcctcg tgccgcccct gacgcggatc cagcagaatc acctcccctg cccggctgac
    34801 cccgtgatgc ccggagacga tgcagaccac gcggtgcgga tcaccgggga tcggacgcgg
    34861 ccagtacatg gcgttgggcc agtacgaacc gcttccgtag tactccatct gacccgaacc
    34921 gtcgggattc atccgcatga ggaaacgtga gaaatagtgc ggtatgtcgg catactccca
    34981 gcgcgtatag agcacgcggc cgtcatgcat caccgtgggg ttccagttgt gatcctggtc
    35041 aaaggcgatc cggcgttcgt tacggccgtc ggcatccatc cggtgcaggt tgccgacatt
    35101 cgcctcgccg gtgcagggca cagcctgata acaggcgtta cagacggcca gcaagccgcc
    35161 gtccggcaaa tagcatccgt cataatgcca cacatcgggc ggcgcgtccg tgatccggcg
    35221 cggcgggtcg tcgggtgcct gcaagtcaag ttcccagagg atcttgcggt cggagaacaa
    35281 gacacgccga ccgtcccagt gcagatcgaa atcggaaacc gttcccttgt gcacacgcgt
    35341 cacgctaccg ctaggcgcca ccggcgagag aatggcgagt tcatcaccga tctgattggc
    35401 tccgccccaa ttgctgttga aagagaccga accgcgggcc agcagcaccc ggttaaacgc
    35461 gagcagcggg gtgtcgagca gcacggtctg ccgcaaggcg tcgagcgcga cagactgtcg
    35521 ggcggcacgt tcaggaagat ccggcccact atgcgggaag gcgcgtagtt cctccgcgat
    35581 ttcccgggat cgcgcatcga tttgccgccg tgccgcatga ccctgcggat aggcatcttt
    35641 gaacatgcgg atatggtctt cgaccgccag acgtatggag atcacggttg ccgcgctggt
    35701 catcagttca cgcagggcac aacttttcag aaacgccgca cggggatcag accgacgcca
    35761 cgccgcctcc ggtttccgcg cctgcccgac ccacgtttcc agcatctccc ggtacggggt
    35821 cagcgcctgc gccgggaggc cctccatgcg cgccagccga cccgcgatct cagccaggac
    35881 atcgttacag gcggtcgcaa agcggtttga agcgaactcc gcctcacatc cggggaccca
    35941 ggcataccga taccggttgt actcctgagg cacccagacc gacgccaacg cctcccactc
    36001 ggccattcga atctgatgcg gcgcggcgtc aacctcggcc aatgcccggc gcatcagagc
    36061 cagacgccgc tgggaacgct gccaactgtg gctggccgga gattgtgtgt tacggcggaa
    36121 cacgcggaaa taaaagcggc gatagccgtc gcgatccggt ctgatgacca tcatgaactg
    36181 gttcggcccc ggacggatgg tcagcgcata cccgtcatcc aacaccggcc atgtcccctg
    36241 ttcatcccgc cggttgcgtc ccggatgcca ctgcgaatac ccgtcgctca gggacacatc
    36301 gagcatgacg tccttcagat cgcaccgctc agccagcgtc accggacgga tcgcgatgaa
    36361 gcgatccccg ggtcccgcat tgcggaaggc cgtcagatca cagaccgcat ggtccttgaa
    36421 ctgcggacag ggaatccagg cgtgccgtcc gtgccgatcc tccaacggaa cagtgaggtc
    36481 gatcaggccg tactcattga taatcttgtc cgtcagcgga gagtcgggtt ttagcggccc
    36541 caacacatgc caaaccccga gttcgagccc ttcgggcgcg gccgcgcgcg catcgtgtgc
    36601 cgcctcccaa ttacggagcg cacccagatt gtgcagcatg gtttcgctca aggtcgcgcg
    36661 ccgcgcaaac tccggcggat tgggcaacgg ttcgagttcc gcaaccgtag tcaggaccac
    36721 cccggcaacg gcaatcaggt tagcggtccg ccaacatgcg gaaaagttct ttgtaaaacg
    36781 agtcatgcgt tcacctgatc cattcggcgc tcacggtttc acgtaagtca gattggtgca
    36841 gacctgggtc acttcgtgcc ggaccggcca gaccgcgcgt tcaggactag cgtaaagccc
    36901 cattttctcg atgggcaggg gacggaaccc cagcttgaaa gcgggagacg ccgactgcag
    36961 gcggtaatcc cctttggcgg catcgataaa aagaggatcc gcacccgcga gattgttttc
    37021 gccgaccagc caggaatgaa ccgtgcgagg atatttttta taatgcgcca accacacgga
    37081 cggtccgccc caaatcacgt tgcgctcgac ccgtgtgcgc gtttccggcg gacggagttt
    37141 tttatcgcgc accgcattac ggaaggccaa cagccaagga taacgttctt tccacagcgg
    37201 atcctccacc ggcgtggcga agaggcgttc gggcatttgc gcatgcaccc ccttcggtgg
    37261 cgtgaccgac tccggcgtct cataggtctg ccatccggaa atcgcgggat ggcaatcaac
    37321 aaacacgttg ttgtcgatca cgttacccac cgccgagagc gagatgccct cggctacgcg
    37381 gacaatcaga ttctgcgtca tggtgatgcc agccgcgccg tcatccagca tgatcgcgcg
    37441 attgcgctgc ggtcgcgggt tatagatatc gtggatatag ttgccgtgga tcacattccc
    37501 gaccagcgtc cagtcgcggc cggcgtaaaa cgcgcccatc tcacccgagg agagacagat
    37561 gctatggatc tcgttgtact ccatcacctg ttcccgcccg ctgaacagca ccgccacatg
    37621 cggtccgtta tgaatcaggt tgtgcgaaac ccggattccg caaccgtaga gctggaccgc
    37681 cgcaccatag gtcatgctga agcgcgcgta atcatggata tggttgttgt caacgacatg
    37741 tccgcagcgc tcgaggctcc gctcattccc accggagacc tggatgcctc cgtcgcccag
    37801 acgcgtcatc tcgcaccccg cgatccgatg cgtttttccg ccgcggatcg tcaccgcccg
    37861 gtgcccgatg ttccgcattg tgcaaccgat cacgttcacc gactcgccct gactgatcag
    37921 gatcgcctcg ccgcggcaat tctcgaacgt cagcccccga aactgcatat gggcgacgtc
    37981 gtccaactgg atcagccgcg tccctaccga cacttcggca cgggtgttcg cagatttccc
    38041 ggggggccaa aagtagagtt tgccgtccag ccggtccaca tagtactcgc ccggcttatc
    38101 caattcgcac aacaggttat aaccgaacca gtgcccacct ttgcggaatc cgtagtggct
    38161 acccgggacc ttctgcgtga tcgtcttcgt tgccgggtcg atccgctcaa acgcgatcgc
    38221 gcaagccgcc cagtcgtgcg cccaataccc ctgtccgcgc ggatcgatct cgtcaatcca
    38281 gcgggaagga ttctcctccg catacgtgaa ggtgtttccc tgtttgccgt ccggcgtatc
    38341 cgcgatggtc acgaatccct cgttgggcca gcgcgccaac cgcatcacct cgtcaccgta
    38401 gatgagttgg atgccggaca caggtccggc agaggatccg accggttcca cgccgtactc
    38461 gtaaactccc tgcgctggca acgagatcac ctggaccttg ccgcgcgcgg cctccggcaa
    38521 ccgccgcagc agattggtgt cggtcaacgt gttcatcttc cacaacgcca caccgcccgt
    38581 gatccgcacc tgcccgttcg gggcggcctc gaaaacgacc ggcgcttccg gcgaacccga
    38641 atcctctttc gtgaaaacaa ccggggtccg ttgctcataa cggccgatct gaaaacgtac
    38701 cgtgaccgcc tgcttcggcg tcaacgtgcc ggcatcgcgc aaccgccgca acgcatcccg
    38761 ggctccgccc agagaggcga gaggcccgtc ggtcttcgcc gcgttcggcg cggctaacgt
    38821 gccgctccac gtgtctttcc cctgcggcga aacgtgcagc gtcacgttag ccgcaagcgc
    38881 accgcacaca cccagtaaaa aacaaagtcc agccaccccg cctcgacaca tcggttcccc
    38941 cctttacaat gaacgattca gttaagcaga cgcttcatcg tttgacaagc gttatgggcg
    39001 gaaacacgcg gacgcatcac ggtttcagat tttcgtatca tgaaacacat gaattttcat
    39061 gaaaacaggg acaggcaaag aatcgggagc gcggccgtcc tcgcccgctg gcagggcggt
    39121 ttccccgcaa accgccgccg gtccctgccg agcggacgcc tccctgatgc gtccctagtt
    39181 atccgtggca ggcctcatga cacaacggaa atccgcctcg gccgccagtt cttctcccac
    39241 atacgccttg ccatgctgga cggccatgcg cttattcacg cgccgatttt ccacctccat
    39301 ccgcagcgta tcgcccggac ggacctgccg cctgaacttg gcgttgtcca cgccgaccag
    39361 cagaaacacg ccgttctgga tcagaccgga ggcaaccagc cccgcgcccc cgcactgcgc
    39421 catcgtttcg atcaggataa cgcccggcac aacggggtat cccgggaaat gaccctgaaa
    39481 aaaccacatg tcctcgccat aggtcatctc tccgatatag ttctgctgat cgaaatacaa
    39541 caagcggtcc acgaacagaa acggtttccg gtgcggaagc agttcttcaa ttgatcccgg
    39601 atattccttg ccgctcgcca cgagttgctg ccatgttatt ttctcttcgt tactcatgct
    39661 catccccttc gccacggatc cggagaggat acggagttta cctccccttc cgcgtttgcc
    39721 gtttacacgg acttcaatac cagaacgcca ttgtgtccac cgaacccgag cgaggtggaa
    39781 agggccgcgc gaatcggtcc gttcaccccc tgattgggca ccacatccag atcgcacgcc
    39841 ggatcgggtg tcgtgtagtt gatggtcggc ggatagaatc cgtcccgaat ggccagcgtg
    39901 cagatcagcg cctcaatcgc gcctgcggca cccagcatat gaccggtcat gctcttggtc
    39961 gaggacatct tcagtttacg cgcatggtca ccgaacaccg tcttcactgc cttggtctcc
    40021 atgggatcgt tcaacggggt ggaagtgccg tgggcgttga cgtaatcgat ctcatccggc
    40081 gcgagtccgg catctttcag ggcaagacgg atcgcgcgga tggcacccgc cgcctccgga
    40141 tcgggcgcgg tcagatgata ggcgtctccg gacgcgccgt agccggcgag ttccgcgtag
    40201 atccgcgctc cgcgggcgcg ggcatgcgcc tcgccctcca gaatcaggat cgcagaaccc
    40261 tcccccatga taaacccgtc gcgcccggca tcaaaggggc ggcaggcctt ttcaggcgtg
    40321 tcgttgtact tggtgctgag cgccttcatt gaacagaacc cgcccacaca atattcggtg
    40381 accgtcgcct ctgtcccgcc cgcgatcatg acatccgccc gtccggcgcg aatcatgtcc
    40441 atcgcgaccc cgagggcgtc cgtgcccgac gcgcaggccg tgaccagcgt gtgcacggcg
    40501 gcctgggcct tgagcgcgat cgagatgttt ccggcagcct cattcgcgat cagtttggga
    40561 atcagcatcg gcgagagccg gcccggcccg cggttgaaca gcacctgata tgcctcgtca
    40621 tccacctctt tgccaccgat gccgttcccg acaaacaccc ccacgcgctc cggatccgcg
    40681 cccccgtccg ccaatccggc atcctgccag gcctgcctgg ccgctgccag cgcgtactgc
    40741 gtgaagagcg ccatgcggcg cgcctccttg gggtcaacgt actgggtgat atcgaactgc
    40801 ttcacttcac ccgaaatctt ggaatccaca cgcgagggat cgaaccgggt ggtgcgtgcg
    40861 atcccagaca ccccattccg gatattgtcc cacatctccc ccaccgtgtt gccgacaggc
    40921 gaaatcacgc ccaaaccggt gatgacaacc ttggttttcc cgctcataag cactggtctc
    40981 ctttttttcc cacttggggt acgctaagca ctctccggat gcggacgctg cggcgggacg
    41041 ccacccgcca cgtcgtgcca ctggagcaaa atcccgccgt aggtcaggcc gccgccgaac
    41101 ccgatcagca caaccaaccc tccgtccgta agcttaccgg aacggaccgc gtcatccagc
    41161 gcgaccggca cggaggccgc agaggtgtta ccggtggtcg cgacgttcat gtggaatgtt
    41221 tccaatggca ccttcatacg gcgcgccacc gcctcgacga tccgcgcatt cgcttgatgc
    41281 gggatcacgc acgccagatc ggcgaacgta tggcccgacc gttcgagcag cccgcagagg
    41341 atctcgtcga acaccttgac cgcgaaattg aaaacaggcc gtcctttcat gaacagcgta
    41401 ttgtgcatcc atggaccggt cgcgagttgc cgcgcccccc cttcacggta caacaggtcc
    41461 gcacccgaac cgtcggcgcg caggatggca tcgcggattc cgccggactg cccgctggtc
    41521 tgcagaacca ccgcgccggc cgcatccccg aaaagaatac aggaattacg gtccgtccaa
    41581 tccacaatgc gcgacatcat ctccgctccg atcaccagcg taggccgggg atccacctgc
    41641 atcagaccgt aaaccatcca cagggcatag atgaacccgg tacaggccgc ctgcagatca
    41701 aacgccgcgg cctgcgtcgc acccaatgcg tgctgcacca gacaagcggt cgaggggaaa
    41761 gcctgataat cagatgtgga cgtggcgacc acgatcatgc ccaactcttc agcacgcaca
    41821 cccgcggcct ccaacgccgc gcggccggcc tggatcgcca tggatgaagt ggtttcgtca
    41881 tccgcgccga gatgccgact ccggatcccg gtgtgcgaaa aaatccattc atctgtggtg
    41941 tccagtttct ccgccaactc cgcgttggtg accacgcgcg tcggcagata cgaacctgta
    42001 ccggtgatat ggacaaacat gccaatcctt tcgtgctgaa aacaacgctt gatcatgacg
    42061 ttttttgttg ttttgtcaac ctgcaatgcg tcgcatctga aattttgtct caggaaaaag
    42121 aaaccgattc gaaacgggcg gttgacaggg aaaacccggc ctgatatagt gaccgctcgt
    42181 tatgggacag caacttagaa aacgtttgaa gcgccagcgc cgggaagccc gcaacaagcg
    42241 gatcaaccag aagctgcgcg ccgcgatgaa aaagtaaatt tttcataagg gtgcgatggc
    42301 cgagtggtta ggcagaggtc tgcaaaacct cgtacagcgg ttcaattccg cttcgcacct
    42361 ccatgtttcc actgatcatc acagattgtg acggtcagtt ttttttgtgg ctgcccttcg
    42421 tgaaatgcag gcgcgcaaaa aaaccgcgcc ccgcgcggtt ttggccgcac ggggcgtggc
    42481 acgtgaaaag aaaaggccga gccgctaacg gcgaaccgcc agcggctctg cgtctcttac
    42541 atgtccatgc cgcccatgcc cgggttcggc gccggagctt ccttcttctc aggaatttcc
    42601 gtgaccatgc actcggtgat cagcagcagg cccgcgatcg acgccgcatt ctgcagcgcg
    42661 ctgcgcgtga ccttggccgg atccagcacg ccgtccttga ccagatccgt gtactgaccg
    42721 gtcgccacgt tatagccgta ggcgcccttg ccgttcttga cgttctcgac gatcagcgcg
    42781 gcttcca
//
LOCUS       DAOSRB010000002        42094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1034_length_42094_cov_5.899998, whole genome shotgun sequence.
ACCESSION   DAOSRB010000002 DAOSRB010000000
VERSION     DAOSRB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 42094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42094
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1034_length_42094_cov_5.899998"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLZ74_00195"
     CDS             complement(<1..920)
                     /locus_tag="PLZ74_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96773.1"
                     /translation="MRKLRSFSILATAAVFGAVAMVAQTLLLRRFLWRFAATESGVAL
                     FLACWLGWTGLGAAVAATRMGRRITGILADRLWLPLSAGIALYFVQYALIGNLRAWLG
                     VPDYQAFPLARLAAGCFVANFPFCFVSGFVLPAVAVRLARDGSPVSRAFAWEALGAAL
                     GGLAVTGLLALGIAPDPRDHAEWRRAFPDGAAPSGRFETGGGTTFYGRHRGSFYALSS
                     SGVREVIPETDRAMGWAALLLSQRPYAHAALLLGEVPLAVGLAMESLRPDLEIVWCPA
                     DPGYGAGLLQSVREAGVATRIRAAGATPRR"
     gene            complement(1032..2435)
                     /locus_tag="PLZ74_00200"
     CDS             complement(1032..2435)
                     /locus_tag="PLZ74_00200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR96774.1"
                     /translation="MMKLTFFGAAQTTTGSMHLVEANGKRILLDCGLFQGHRKEAFEK
                     NRNMPIDPRTLDAVVLSHAHIDHSGNLPTLARHGFRGKVYCTPATRDLCDIMLRDSAY
                     LQQRDLEYVNKKRAQQGKVLFEPLYEQEDVDHILSRFEPEPLKRLVDLGDGISLLLHN
                     AGHILGSALVQLDVKTRGGTSRRLLFTGDLGQPDRPILRDYDFPQGADTILIESTYAD
                     RDHPAREDVSGRLKGFVEDIHQQKARLIIPAFSVGRTQHLLYELNRLIEQRRMPLTPV
                     YVDSPLSLKATQVYARHRECYDEEASDLLRQGIDPLRFPGLQFVETPDQSKALNDRPG
                     PMIIIAASGMCEGGRILHHLRQGVGDPRNIILIVGFQAENTLGRRIVERRSPLKILGD
                     EFELNARVHTINALSAHADRTALMRWFDAVRGKRLKRVFAVHGEPEKVQAMIELVRAR
                     GVENVVAPQPGQKFEDV"
     gene            complement(2489..2686)
                     /locus_tag="PLZ74_00205"
     CDS             complement(2489..2686)
                     /locus_tag="PLZ74_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96775.1"
                     /translation="MRDAGRLVRWGRGMTAVRREEHEDFLGAWIEEFCGMGVPPMKHG
                     QDARAIPAFGVPVILALRWIE"
     gene            complement(2776..3234)
                     /locus_tag="PLZ74_00210"
     CDS             complement(2776..3234)
                     /locus_tag="PLZ74_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96776.1"
                     /translation="MATSKRSVKRTSVKPAGKKTAATAARAAAKPEPRKAAPQPVSAA
                     GVRPPVAVERPAAPSTGAVAGPTARTCPLVLDGETSAAAFTPRACSACDEFDCRFNVT
                     EEGSGALRSRLFAVEEEEEDGGEDVLYGADFDEEGDGGFGGADEEDDDLF"
     gene            complement(3278..6484)
                     /gene="ileS"
                     /locus_tag="PLZ74_00215"
     CDS             complement(3278..6484)
                     /gene="ileS"
                     /locus_tag="PLZ74_00215"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOR96777.1"
                     /translation="MFEPVSKQTDFPGMERDMLAFWESHDTFRKSVEQRRGQREYVFY
                     DGPPFATGLPHYGHLLAGTIKDIVPRYQTMRGHHVERRFGWDCHGLPIEALAQEALGV
                     SGAQAIREFGVAAFNEQCRSMVLTYVEEWRKTVTRMGRWVDFDNDYKTMDPGFMESIW
                     WVFKQLWDQGRIYKSYRIMPYSWKLSTPLSNFEAGNNYKDVQDPAVTVRVRVRSGAEP
                     AWGTTYLLIWTTTPWTLPENLAICVGPEIDYVVVRDCEDETVFVLAEARLPVYYKDAG
                     AYEVLARLKGEALRDWTYEPIFPHFAEYRDAGAFRVLTDSFVSTEDGTGLVHMAPAYG
                     EDDFRVCQQAGLSVIADPLDAACCFTEAVPECQGRFCKDCDKDLIQWLKREGKLVHQT
                     TVTHSYPFCDRTDTPLIYRAIEAWYVRVEDLHERLTRNNATVRWMPEYVGEKRFGNWL
                     NEARDWNISRNRFWGSCIPVWINENDPADQICVGSVAELEQLSGERVTDLHKHFVDRV
                     VIRRDGKVYRRTPEVLDCWFESGAMPYAQQHYPFGDLGVRSEDLGVEDLGVEEKRGKG
                     GQDISTFFPADFIAEGLDQTRGWFYTLMVLGTCLFDQSPFKNVIVNGLVLAEDGKKMS
                     KRLKNYPDPSHILDAYGADALRLYMIYSPVVRAENLRFSETGVKQILRELLIPWWNAY
                     SFFVTYANVDRFDDAEVVRPESPNVLDRWIVSSLETLIRDVTVAMDGYDLQRSVRPFV
                     TFIEDLTNWYIRRSRRRFWKSQNDEDKTHAYRTLRYVLVQLCQVAAPFTPFISEAIYR
                     NLRGASMPESVHLCDFPQAYAAGRDEALESAMARVQQVVRLGRQLRTENDLKIRQPLA
                     VLHVVSGNAGLRAILQGYEDLITDELNIKTVAYGEDETVMADVSVKADFRQLGPRVGP
                     RMKAAAAAIAALSSAEAAALAAGREVSIAVGGESMTLCAGDVVVRRTPKVGMVVASED
                     DVIVGIETALTPELIREGLAREWVSRVQNLRKEAGYEVTQRIRVTVACGAEVREAIEQ
                     YADYVKAETLCEGIRFDEVHGPVIELNGHAVGLHVEKA"
     gene            complement(7245..7982)
                     /locus_tag="PLZ74_00220"
     CDS             complement(7245..7982)
                     /locus_tag="PLZ74_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR96778.1"
                     /translation="MRLTVVIPVYREYRRLKGTLEQVAAFIRDTTEVKVDAILVDDGS
                     PDRCSDVVREFIAASGSDRIRLLGYKVNRGKGYAVRTGVLAAEGDLILMSDADLSTPL
                     QEWVRLKEAVDAGAAIACGSRAVRGAHILTPPPLHRRVLSRVFNLLVRLAGVHDMRDT
                     QCGFKLFRRDVAREVFSRSRINRFAFDVEIIAMARDLGYRVVEVPVIWNYGGHSTVRV
                     FSSGGRMLLDVLWLALRRAVAGPARRV"
     gene            complement(8061..10688)
                     /locus_tag="PLZ74_00225"
     CDS             complement(8061..10688)
                     /locus_tag="PLZ74_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOR96779.1"
                     /translation="MITRGMRRAAAALAVAAIPVSPVWADGCGASGSATDAAARFILQ
                     LGVLVFAARLGGALFERGRLPMVFGEVAAGVVVGPYLLGGVALPFFPHGLMGDAEAFL
                     SVWGGGGQGLMAVILAVFFFLAGLDTDLRQMRRLSASGVLAGVCGFLFAFGAGLALLF
                     RAAPTLLGPAQPWHTSALLLCAVPISVTSVGFLARLLAVRKRLESPVGTLSLTAAMAG
                     NVLGLIVFTVVAGVSGAGGGVNELSGARIAAVAWRAGVGLGAVLLLGIPLARRMNALA
                     VREKNYGQPFAVSAAGLLIAGGLIGACGLPVVSGAYMMGVAFSAMDVRHEIRERLDFV
                     SLTLMPACFASLGMRLNPNLLANETVLGTSVLLAVAVLGANLIGCAAGASLTRSGVAT
                     SLRVGILALPRAELSVALLAAVGGMIPLPQSLLFCAVFLILCSCVALPFLSDRVLDQG
                     DGCGGSGPEPARLVFRFPSRQAAQLMANRIVELFEDEGFYAQVLNRHHMLYRISRETQ
                     VIHLQSHDGEITFECGERERALVNVVMLEALSDIEGNLCALRRPLDDVLLRKNIQVPE
                     ASAAGSAGVLRNHLFPETCRPRLLASTPAGAVAELVNLLCESGLVTDRDRALQAVLDR
                     EQNLSTGLEHGLALPHARTDAVTRLVCAVGLKKEGLAYDTVDGRPARIVALVLAPEQA
                     ASPQLQVIAHLCRLLDSHGRAALLACDTPDDMYAVLTGGRAGAAFCARQTILARCLRW
                     QSIALDFDVEDRQQGLALLLALCARSGAVNALPEAMADIRRGVETPPVRIGEEVAVFP
                     VRTAHVDRMVAALGIASATSGSGTSPRVWVMVLYPESEATVYAEVEAELRQRLTGQTL
                     DGLLAAKTAHEAFALLTGG"
     gene            complement(10707..11369)
                     /gene="larB"
                     /locus_tag="PLZ74_00230"
     CDS             complement(10707..11369)
                     /gene="larB"
                     /locus_tag="PLZ74_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOR96780.1"
                     /translation="MTEDLHFAKPDFDRQRRLGMPEVVFGSGKTAVQIAAIMRALNDA
                     GQNAFVTRVTPEQAAAVQTEIPDAVYREEAHILTRDVAPLPQPEGKVAVLCAGTSDLL
                     VAEEAALTAERLGARIERVYDVGVAGLHRLLSRIDRFEDARAIVVVAGMEGALPSVVG
                     GLTDRPVIAVPTSVGYGASFQGLAALLGMLNSCAAGVTVVKIDNGFGAGVAAARINRQ
                     GA"
     gene            complement(11373..13142)
                     /locus_tag="PLZ74_00235"
     CDS             complement(11373..13142)
                     /locus_tag="PLZ74_00235"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOR96781.1"
                     /translation="MSNTSSLDVIASLCKRRGFIFHSSEVYGGINGFWDYGPLGCEMK
                     RNIKEDWWKTTVRNREDVAGLDATIIMHPAIWKASGHLDTFSDPMCTCRACKKLMRAD
                     QVWDILAEGAIGQSLKALLEGGADAAQVAGWCKGKAKGLAPNLAAVLDADTLTAAYET
                     LKADPAPYAAVRALYTLLANAKAPQPAVVPCPYCGGELTEPRPFNLMFRTVVGPVDDS
                     ANIAYLRPETAQAIFAQFQNVLATSRQQVPFGIAQMGKSFRNEVTPRNYTFRSREFEQ
                     MELEFFIRPDEAVELICGHVTTLDDHPDLSVPQDSWGWEVWHKYWVEQRKAWLEACGL
                     PKTSFVEYWQKPEELAHYARACVDIEYRFPFGVQELEGIAARSDFDLSQHQKHSGKSM
                     EVFDEPLKLAADKLDDAAKLAFTDKVVAEWRAHGKDESDARAFVSKLFLGKYVPHVIE
                     PSAGVDRMMLALLCNAYEEQKLTDDKGKEDTRIVMHFSPRIAPVKVAVFPLVKNKPDL
                     YNRARGIFEDLRRRYSAFWDEGGAIGRRYRRQDEIGTPWCVTIDFQTIEDGTVTVRDR
                     DTMLQERLTIEQVKAKLDDALLV"
     gene            13606..14388
                     /locus_tag="PLZ74_00240"
     CDS             13606..14388
                     /locus_tag="PLZ74_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR96782.1"
                     /translation="MMRITRYVLCAGIFSAACCLAAEEGWTPLFNGKDLDGWIPKIKG
                     YPAGENFANTFRVEDGMIKVRYDGYDGAFKNRFGHLFYKTPFSHYILRVEYRFVGEQL
                     PDGAGWAKRNSGVMIHGQTPESMGVNQSFPVSIEVQFLGGLGSGNRTTANLCTPGTNV
                     EKDGKLYTPHGLSAKSKTYHGDQWVTVEIEARGSETILHRIEGETVLQYDKPQYDPKD
                     GDARRLIEANGGKLLIEGGTISLQSESHPIDFRKIEIKVLKP"
     gene            complement(14369..17212)
                     /locus_tag="PLZ74_00245"
     CDS             complement(14369..17212)
                     /locus_tag="PLZ74_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOR96783.1"
                     /translation="MLNFRTVWVTRHPGRPPVFDLFPVTDQRDGGETVYVTQDPAAFE
                     GVLPQEGAWAVDVLVLYAFAFPGTPAEQVTTQIRQGPHTGEAIGVLWQAVAARLNALP
                     LWTLESIEGILRNLDEHGLARVFSHFAACVRQAGVGCGPWIETFAPEARRGDRRALPE
                     HADCSPLDVAAMAAHLLPGGAFARLMPGYEPRAGQVEMLHAVTRAFNEGRHLLVEAGT
                     GVGKSLAYLIPAAAWALLNDVPVVVSTNTRNLQAQLIENDLPRVREAVCPGGRAALRV
                     AVVKGRSNYFCLRRLAILLEQSQYELDRPEWRHFAYVLAWAAQTPDGDLDTFAGPGRV
                     DAEFLAKLASTAEECPGRACRYSRRCFLQRARARAAAAHVVIANHALVFAESRAPGTA
                     LPPHAQIVFDEAHNLEEAATRHLSTEITQTRLTQLLRRLSRGRGRRAGGMLEVLRNHL
                     EKGAVTADPEYAQALRRQIREIRNVLEEVQAAARQLFESLHGLIQPEPGPVRFRCVPC
                     DGPDATVWPTGESALAAGNAGQPACGGVFPSGFRREVFRDKRFTPCPDAWDEDTVQMH
                     RIGLKNAIAEASGLLLALGEALRQATEGELALYDDQAANVEGAAASLRTYAMDLDFVL
                     AAYDAEHVFWAEPPSRSAGTAHLYAAPLRIGEALAETLYRRKATVVFCSATLRVGNSF
                     GYISRRLGIDRLEPDRLLTCVAASPFDYLTQCAALAPVFLPEPTGGTGGTYAEQLSGL
                     MLDVFTRTRGRAMGLFTSYEMMNRVARLLEEPLQEAGIRLLVHGTNGSRDQITRIFRT
                     GGNCVLLGTHSFWEGVDVAGEALSCVVMARLPFAAVTDPIVEARCEQIEDAGGCAFRE
                     FSLPQAVIRFRQGFGRLIRTRADRGVVIIADPRVVTKSYGATFRKSLPCPLLRVETRE
                     ELLRRVEALFAESAMAQESARRHGAGDTTASAP"
     gene            complement(17237..18106)
                     /locus_tag="PLZ74_00250"
     CDS             complement(17237..18106)
                     /locus_tag="PLZ74_00250"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOR96784.1"
                     /translation="MSRAGVLSFVVSKSEAGQSVQVFLAAKLSRSRRAVKALLDERVV
                     WVNRKCVWMAHHTLRQGDTVELPAAVLRKGARDANPVAVPKIRLLIEDAEYAVVDKPS
                     GLLTVGAGSVEERLRAQTGIAELKAVHRLDRDTTGCLLCAKTPEAFDAAVELFKTRRV
                     TKQYHTIVSGRYERHASTVDQELDGERAVSHIQRIMLSRDASFLKVRIETGRTHQIRR
                     HLAAIRHPVLGDRKYGVKVARDPRLLSVPRLMLHASGLGLPHPMRPGKTLQAHSPLPA
                     DFRHCLKLFDMGK"
     gene            complement(18136..18855)
                     /locus_tag="PLZ74_00255"
     CDS             complement(18136..18855)
                     /locus_tag="PLZ74_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR96785.1"
                     /translation="MTSVCAMRDHKSLYRQLIAGMYDALLITDPNGHVIEINPRATEY
                     FQVKPEEVWDKPVAFLIPGVTPQMIGRIRKGIDEARHIMLDAKCQRQDGTVFAAEVTI
                     SVIDLINSGDLVFTVRNTERRRKQWQALRSMANAFENAQSACFVCDRDRVFRCVNPAF
                     LKMFDLREENEVLGRPFEDLMPDEPLPELFNRALGGEPVACRVVADAEEDKRIDLEVQ
                     MGPNRLGRDAVAGVVGSLLQV"
     gene            19035..19292
                     /locus_tag="PLZ74_00260"
     CDS             19035..19292
                     /locus_tag="PLZ74_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96786.1"
                     /translation="MEERLDETTFVSFICEACNQEIEAPADLAGRETECPTCGVTLTV
                     PYVSTPGTLWGGPLENTGGPTPAQTEAMKSRTIRIELPDSW"
     gene            19300..20478
                     /locus_tag="PLZ74_00265"
     CDS             19300..20478
                     /locus_tag="PLZ74_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR96787.1"
                     /translation="MSRILFISPKPYSPWEGACHRTRHILEAQIALGYDVDLLTPTEG
                     QTPTVADSVTTIPLPRLPFCRRTPTLPSLLRLTLDLLALLKILWLAGNTRYALIHGQG
                     NSGVLAWAAARLLRIPCIVELRNDPAPDPAHRRGLIPALYRRLEHKALRQADAVIGHD
                     AATVARLTDLGCHSRACVIADIPAVTEVADAPARNLAKARYRTLTDQDLVTCVASYTR
                     FQGLDLFFNALPRLLATLPHTRCVVVGGGPEEIAQMREALDRAGIHEAVTFPGRLSTS
                     ELAALLAISDVLVSSRRAGATAPIKVLDYLLSGTPIVAVDTPANRAILSPENAVITPA
                     TPEGLADGLIRLLRSPGLRTELRLKGRETLRRENRTPEAFRAALARCYTYALSRSEAE
                     "
     gene            20480..21520
                     /locus_tag="PLZ74_00270"
     CDS             20480..21520
                     /locus_tag="PLZ74_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOR96788.1"
                     /translation="MTRRLLRFARSFVILVSLFLLSTLIAANALVHLPRQRQQSLLQN
                     LPDVAAQTLTRVGHAAADITDGLGWTGRDAVAALPQPTDTNRLTRAGVPRRLPSCTAP
                     DDLVVLNKIGFTIGYSPAWRHPVWVAYRADPTCRNPLLPRPSGFKPDPAAPRSPKHRE
                     YSRSGYNRGHMVPNRVIASRHGPAAQAQTFLTSNICPQRASLNQGPWENLEFRIAELW
                     PTRYGSVWVLAGAVPAPEARRLPSGIHVPAAFYQIVVTQPQDGTLRAFAVYMPQHVRR
                     RTPVRSMLVSIDELETLTGFDFLSELPDEVEAELEAATPTRLWPVGLVGTIRLVRERF
                     RRYPVKRVQGAR"
     gene            complement(21505..22341)
                     /locus_tag="PLZ74_00275"
     CDS             complement(21505..22341)
                     /locus_tag="PLZ74_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOR96789.1"
                     /translation="MRMGCVLGVIALLVCGAGAAEREGWRLVWADEFDRPGMPDPAKW
                     TYEKGFVRNREAQFYTVARRENARVENGCLVIEGRKETFANPGHKSGSEDWREAETAA
                     YTSASLTTEGLFAVTYGRVEVRAKLPRGRGMWPAIWMLGTSFRSIGWPRCGEIDIMEF
                     VGHAPATVHANVHWFDTTQGKRTSLGGKLHDQKPSDDFHLYVVERFEDRMDFYYDDTL
                     YHRFLLDKAGAGEDNPFRKPHYLLINLAIGGAWGGQKGIDDAMLPQTYEIDYVRVYQR
                     AP"
     gene            complement(22485..24524)
                     /locus_tag="PLZ74_00280"
     CDS             complement(22485..24524)
                     /locus_tag="PLZ74_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96790.1"
                     /translation="MRKRGIFVCACLISMVAGAVTNVFEMPLLYPKHLALQQQLMRVV
                     RTGETEEMERICREGVALLPRDPTWQYNLACALAYRADKGEALAALDRAIDLGFRDAA
                     AIEADRDLKSLGPIKAFRELLDKARRLQGKPVEGMPSVRPVSVVMGRPAEVNAENTIW
                     DFEFGCFRTRFNLLVPDLRPVSDYAEAYRGAAAETVRAGFKAGTASGNFGDLYVNRDG
                     NHSLLVVSNYPGLTPVVYGEQAKQAQVHLSLPNGLFDAPVLGNASLSMVSGPYWRSLP
                     RAAVTDPLRAIISLRFYLGNQIWVFPEHRDHDPETGDLYPVNAPFFVISQGSSGSDQP
                     FVNAFAATLAAFTPATKRALTQRNRIGPTLQRILRITQKAVAKDEERYLTGAAHPVVF
                     DAAQLDVEAMVRMAQAMTPEAIPPLVALRTVEDAKVQAGLDFFDLRSEGLFDTPCCIA
                     RVVRGVSRERVMTVEAAVPGQQQEGAFRWTVLQGDTRKIVITPLNANASRVKLTVSYH
                     GAFRPVDDQGMPSALLRHRVDIGCFARHGKGYTAPSIISFYYLPNEVRAYRDDGQVLS
                     VDYTNAEHRYADPMLTFEKGWKDLYAYDDQGRLTGWYRTRPGRTPERFTYLGHKVLQA
                     DSRDRPVQACAVEYLPRQAGGQQTPPTLTCVETRQRFAYSYADDADRIGRATAEP"
     gene            complement(24562..25362)
                     /locus_tag="PLZ74_00285"
     CDS             complement(24562..25362)
                     /locus_tag="PLZ74_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOR96791.1"
                     /translation="MKGWLLVLFALTLKACAQFTVEERFHSCVFTNAPTGETFAYRLS
                     APQFPRPGVRYPLILFLHGSGECGTDNRRHLKVGLPALLSTLLRQPEPVMILAPQCQT
                     GNWWVKRLARGEDYAMPPQPAPSMEVVLAMVDHLVDACQADPDRVYITGLSLGGFGTW
                     DAIQRRPERFAAAIPVCGGGDIRRVRELRRMPIWVFHGTADQNVDVACSRRMVQALKQ
                     VGNRAVRYTEFEGAGHAVWDRAYGNDEVIQWLMRQTRAPVPWWRRLFG"
     gene            complement(25487..27610)
                     /gene="hemC"
                     /locus_tag="PLZ74_00290"
     CDS             complement(25487..27610)
                     /gene="hemC"
                     /locus_tag="PLZ74_00290"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOR96792.1"
                     /translation="MQTVRAKVGTRGSRLALAQTRDALNRIAALFPFLEFDPVTLETP
                     GDRDLATDLRVSPADFFTRDLDDALRKGCIDLAVHSAKDLPDPVPPDLDWFWLPWGED
                     PRDAWVLPQGKTWADLPEKPVVGVSSERRGAFARARFPHAVLKPIRGAIPSRLEQLDA
                     GRYDALLMAGAALNRLGLEERVSEWIPLSELPVPAGQGRLAVTFRLGDPVWTRVRGYF
                     VKAVRFVGAGVGVAESCTWGGVRDLRQAEVCLYDVLMDDRLLEFLPPSAERVFVGKRC
                     GEHAVRQPDITDLIAKYARQGRRVVRLKGGDPGLFGRLAEETDELERLELPYRVRAGV
                     SALTVATTGTGMLLTKRGVSRGFTAFTPRVEGGAVASVSLEARRRLPLVLFMSVRVAT
                     EMAGQLLAEGWDGKTPVGLVFDAGAEDELIFRTTLAGLREGAAAPVETKAPGLLVIGE
                     AAAGAYRRDTGALRGCRVLLTCSEALLDKTALRVVDFGGVPLLRPLIRLTPCVEAERA
                     LTRIASFDWVVLTSPSAVHLFMGMIRQAGVDLRRLPRVMACGPGSAAAFRHHGIHPDL
                     TPPMEYSAAGLAAEMRTLEIAGCNVLRLRSEKAGPLLADVLREEGAAVEDVLLYVNEP
                     VHYPRLPASDAVFFASASAVEAFMGQAGVEALAGQIVVTIGRPTSEALAGFGRQPDAV
                     AAEATAEGAIEALARVVMSRGLGAS"
     gene            complement(27750..29276)
                     /locus_tag="PLZ74_00295"
     CDS             complement(27750..29276)
                     /locus_tag="PLZ74_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR96793.1"
                     /translation="MRDTRPVRKTFPINAMIERRRVLVVGGGRVGQRKVELLLDSGAE
                     VRLVCPDCVAELQTLAETGAIHHTARRFEPADVEGAAMVFACTDDKRVNRTVLESAQA
                     NRILCCCADGNWPDGDFVTPAIIRTGDVLVAVSTSGKSCRQSRLVKENLRKHLDSIEN
                     SDLLVLGTSHEYLSAEERAPFHLLLREREAIGGMIRQIWGVHEFFILNTCNRIEVVAA
                     ISREAGTSGILRRLLRFDQLPPDRFYMKRGFDAFAHLCLVAAGMDSQTPGEFHVVSQV
                     KEAVDEAVTFGWAGSVIRELCDAALHISKDIRHEVEPLLDVAEIEDLALRYLAEQLPA
                     LPDSRVLVIGTGVVGQGLMEDLLRRGCHCVWAYHRNAPASSAQDAHRIEVIQLKDLAR
                     VLPTIDAVVSAVNASEPVLNRLEHGARLNPRGVTLIDLGMPRNIDPALDTFGNGVRVA
                     DLDEIKRWYRSANGTLDKVLKACDAILREHRSLYERMRRSVQGEHETGEERRTLNVER
                     "
     gene            complement(29340..30176)
                     /gene="dapF"
                     /locus_tag="PLZ74_00300"
     CDS             complement(29340..30176)
                     /gene="dapF"
                     /locus_tag="PLZ74_00300"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOR96794.1"
                     /translation="MKIRFTKMHGAGNDFVLIDDREGTVPWEDHFLMAALAGRHTGIG
                     CEGVILLQRSDKADFRMRFLNPDGTEVEMCGNGSRCAAAFAHAIGASGRALTMETMCG
                     LVDAQLTKNGVCVWMPEPTRKNYGIEVQIDGETIRGDFLNTGVPHFVVQVPHISAVDV
                     QGLGRALRRHPVFAPDGTNVDFVTLRAPNRMTMRTYERGVEAESGACGTGAVACVVVA
                     VETAGFTLPAKVKTSSGYDLTVDGDWRHRKCTGLTLSGPVKFVFSGEIDLDNMELGDA
                     ID"
     gene            complement(30203..32350)
                     /locus_tag="PLZ74_00305"
     CDS             complement(30203..32350)
                     /locus_tag="PLZ74_00305"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOR96795.1"
                     /translation="MMRTCRFFGLALFVLGMMPRIAGAAQTTLVPQPHYGGVAQKVAR
                     VLPAAHLSQKPLNDAISQVAWTNLLNAFDFDHSYFLQSDIDAFIPMRTQIDDALKAGD
                     VSFPFEVYRVFRKRVTERYQAVTNLLEQGFTFDQEESYTWKRREAPWPATLTEQDDLW
                     RKRIKNEVLSQRISRELDRLGVTNAPVQAVTNAMPGAESTVTDTPEQNIAKRYKQLMI
                     VVQDMDEEAVLQRYLSAVAMAYDPHSDYMSPMRKEDFDIDMNLSLCGIGAQLRSEDGA
                     ALVVEIIPGGPADRDKRAQRLTKGDKIIGVGQGNGPIEDIVHLPLNKAVRKIRGEKGT
                     RVVLEVIPASEPSGTITKRIDLIRDEVKLEEQAATGRVARVTSPEGKTVKVGVVRLPT
                     FYGTMDKRPGQAGFRSATLDVARQLAAFNSEQVAGLILDLRNNGGGSLREAISLTGLF
                     VRSGPAVQVREARQLVVLPVPNLDPAMAFRKPVVVLINRASASASEIVAGALQDYGRA
                     VLVGDTQSHGKGTVQTVMPLGSEKMGSMKVTTASFYRINGASTQRRGVAADIVIPSTL
                     DGLDIGEDKMPGALPWSRVESALYIPVSDVAAFVPQLKAFSAQRLADNSRYARYCTLV
                     RHVQELNEKTEVPLEWEARRAMMRAEREMRKIEDEASDASEQEEWEDDVVLDEAMHIL
                     ADLVALSGGGEIPMETEGDLRTRMMRIFGRELP"
     gene            complement(32347..33054)
                     /locus_tag="PLZ74_00310"
     CDS             complement(32347..33054)
                     /locus_tag="PLZ74_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOR96796.1"
                     /translation="MQEPESARIYGVPGERARAAGVMRAVGPLLAALFVLGLFSGLLL
                     PRITWEAAGLGLLVTAAALVWAVRDGLAGLDAFFKGARGEERVAWVLSGLPRGFHVFH
                     DLLCGGRSEGIDHVVVGPTGLFAIETKCWSGSVTLEEGVLRVDGGLPSRPPLEQARAS
                     AAAVTSFLAERVGGSPACVPVVCFASNTFRPGVSVCEGCVVCNVSGLAGVMRERGGHL
                     SADEIERAVKVLEQKDV"
     gene            complement(33080..34972)
                     /gene="dxs"
                     /locus_tag="PLZ74_00315"
     CDS             complement(33080..34972)
                     /gene="dxs"
                     /locus_tag="PLZ74_00315"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOR96797.1"
                     /translation="MSNLLKTIRTPADVQVLESGQLPVLAEEIREAILETVSRNGGHL
                     ASNLGAVELTIAILRAFSPPQDKVIWDVGHQTYAWKLLTGRRERFHTLRQRDGLSGFT
                     RPEESDCDVCVAGHAGTALSSALGLAAGRERGNGAGHVVAVIGDGAMANGIALEALNS
                     IEEVRSKVIVILNDNEMSISENVGALSRRLGRMLADVRYNRIKAAAEAAGHRLRMTPL
                     RRIYYRLEQAIKSLWLQNAFFEAFGLRYVGPIDGHDFRALENALCSAREYKRSVLIHV
                     ATQKGRGFRPAERRPSAWHGVGAFDLEAGQPPEARPGGYSQAFGETLTALAERDPSVV
                     AITAAMCSGTGLEPFASRHPERFFDVGICEAHAVVFAAGLATAGLRPVFAVYSTFLQR
                     AVDCVMQDVCIPRLPVVFCVDRAGVVGGDGPTHHGVYDIPMLRCLPNLTIMQPKDRVE
                     LEAMLRTALALDGPSVIRYPREPGPEVACDAAVPALSVGQAEVLLHPDAGPEGGEVWI
                     WALGDMLPLARDVATRLRDAGLRSGVVNARFVKPMDTTLLYAQAGNAAQFVTMENGAV
                     SGGFGSALREALAAGGFDCPVSAFGWPDRFVGQGNLAELLEGCGLTAENMARQIAGKK
                     RVATGV"
     gene            complement(34977..36605)
                     /locus_tag="PLZ74_00320"
     CDS             complement(34977..36605)
                     /locus_tag="PLZ74_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96798.1"
                     /translation="MLRKGSHRFCLGCAATLLAGATVVLYAFAVWFGNLNQDEGWYLY
                     AAIRVAEGARPYRDFFFTQGPLLPYVYALFSGLWTPFGVLGGRILTALMGLAGCVMAA
                     GLARRAVPAQRAAEAGLIAFALTGCNLYHIYFTTIPKTYALASCLLLGGYGVLSLCVS
                     RRCKTRSALSCMWALPAGLLIAFAAGTRLSLGALLPVTALALLVTCRKTGAAFFWFAL
                     GGGVGLALVYGPCLMEAREAFIFGQTYHVSRGGRDWFYLAGSFSRTVRAYLPMALLTA
                     ALIIFRIFRSGIVGQNAQEEPAGVRYQGGMWPAIWTLGFLAVFLVHLLSPFPYDDYQV
                     PVMGLLAAALAGWVANSTSSGTLRGCLCLFWTVASLLAAFASPLAQEWFVHGQDRFWV
                     VRKPMPDLALLRMTARDVRALAEEDRLLLTQDTYLAVEAGMRVPDGLEMGPFSYFPEL
                     GDEDAQRFHVMNTRRLSELITSASCRVAAFSGYGLAVRAPVMDEVPHAERQMFFSKVG
                     KYYDRVQEVPGFGQNGTTLQIWKRRTSAAEKAAE"
     gene            36859..37776
                     /locus_tag="PLZ74_00325"
     CDS             36859..37776
                     /locus_tag="PLZ74_00325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOR96799.1"
                     /translation="MNAFPDSVTPPPAALPRDTPGERLQNVLAHRGVASRRHAADIIA
                     NGSVTVNGKVIFAPGFRVDPTRDDIRVNGERLAKTEERSRTILLHKPAGLICSADSSR
                     GQTVCDLMRPHFRERIVPVGRLDKESEGLLLLSNDGELIHRMTHPRYGHSKTYLVRVA
                     GRMAASKIELLRSRLEIDGYTIRPVKVEVLKEGRDNTHLLAFTLSEGRNRQIRKMCSL
                     AHFVVLNLTRVRIGPLKIGTMRPGEWRELSEAEVRALKRTPSASVIPPPDRGGPTRGG
                     TPPHARPKPDLRPPGPRKSGPSARCARHP"
     gene            complement(37718..38707)
                     /locus_tag="PLZ74_00330"
     CDS             complement(37718..38707)
                     /locus_tag="PLZ74_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOR96800.1"
                     /translation="MDMQMNRRAFMMGVAAAGAVTGMPRFGVAQEGGGACAFRTTLYK
                     ALIAGVADDKTCERIAKAGFPGVELSNKNVTEEQAIQGRLTAAKHGLAIHSFMGGWTN
                     FNSSDEAQRRKAIEDVKRMIRLTATYGSSVILLVPCRVGGTMPKPSQFRIAFDPRTLR
                     VTSVVEGDNAPFAEYIAAQNQATEMSRRAIEELIPVAAREGVIIALENVWNNLWVLPD
                     FAAAFVRSFDNPWVKAYFDLGNNVRYAPTEEWLRALGSQIVKLHIKDFKIDREKKNEG
                     DFVPIGKGSVDWRSVRKVIDEIGYNGWVTIESRGFTDAEHSALMDRFFAGQGA"
     gene            39078..41624
                     /locus_tag="PLZ74_00335"
     CDS             39078..41624
                     /locus_tag="PLZ74_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOR96801.1"
                     /translation="MNRNAGTLLAIVLSLQVRAAGDPAAVAAWEKSGRAETLEWFKRH
                     WFGVRPAERLADQEIGEDYVACHGGQIRINIYVKLPEGASAENPVPAFIFGDHYNPKT
                     SRTKTDTMPGIPTNAIVSRGYAYIRFNFNDVTPNTNQGCHDPSSANMKLGGMGNPDSW
                     GTISAWAWGFSRVMDWVETRPELDAKRIAVIGHSRGGKTALWAGCTDQRIALTISNCS
                     GTGGARYLHMPLKGMETLDWMKDHATRHWFCPSWIPTYRNRETEIEHDADEMIMLIAP
                     RRVYVASGSEDSWAGPAGEFESAKRASALWEAYGHKGLSLDTFPVPGTVDHSGRVGYH
                     LRPGAHAIRPWDWDRYMDFMDKHLPGPKKAPPKAMEPAAPESQRVQSEAILAWINALE
                     AFFDGGEQGSVHGFSILRHGKVIAEGSWEPFDTLHETHMLYSLSKSFASTAIGLLVDD
                     GKLDLDERITDLFPEYVPAEPSENLKAMRVRDLLSMNVGTSHADPHKKDPGGDWVKMF
                     VTNVIDGKPGQAFRYDSSATHVLVAIAEKRSGRTFLDFIGERLFDRIGIGKNAWTTYS
                     PTGIACGGWGLNLTTRDLARFGQLYLQRGVWNGERVLSREWVELATARHTWNGGIVGT
                     RPDDDWAQGYGFQFWRCVPEGVYRADGAYGQYTIVLPDQDMVVSMHAGVKSMGKILKL
                     TWEHLLPGVRNVPLPVNTVGEEALQKRLGGLAIAPVKGSVAEGAYFGRELALKENNRG
                     IRSIRLDREGEGWKCTLVTRAGENAFPVGCGVWAKGTIRIDTEKYEKPGGLIGVQPTV
                     ASGGVDADGVFRIKVFLTGTTAYLSFEISEKQILGRLFAIDGCRFEGKTE"
BASE COUNT         8397 a        14746 c        11710 g         7241 t
ORIGIN      
        1 taacgccgcg gcgtggcacc ggccgcccgg atccgggtcg ccacgcccgc ttcccgcacg
       61 ctttgcagca gcccggcacc gtagccggga tccgccgggc accacacgat ctccaggtcc
      121 ggacgcagcg attccatcgc gagccccacg gctagcggca cctccccaag cagcagcgcc
      181 gcgtgggcgt acgggcgctg cgacagcagc agggccgccc atcccatcgc gcggtcggtc
      241 tccgggatca cctcgcggac cccgctggag gaaagggcgt agaagctgcc gcggtgcctg
      301 ccgtagaacg tcgtgccgcc gccggtctca aaacggccgg acggcgcggc gccgtcgggg
      361 aaggcgcgcc gccactccgc atggtcgcgc gggtccggcg cgatccccaa ggccagcagg
      421 ccggtcacgg caagacctcc cagcgcagcg ccgagcgcct cccaggcaaa ggcccggctc
      481 accggggatc cgtcgcgcgc caaccggacc gccaccgcag gcaacacaaa cccggagacg
      541 aagcaaaaag ggaagttcgc cacaaagcaa cccgccgcaa gcctcgccaa ggggaaggcc
      601 tgataatcgg gaacccccaa ccacgcgcgc aaatttccga tcagcgcgta ttggacaaaa
      661 tagagcgcga tgcccgcaga caacggcagc cagaggcggt cggccaatat ccccgtaatc
      721 cggcggccca tacgcgtcgc agcgactgcg gcacccagcc cggtccaacc gagccagcag
      781 gcgagaaaca aggccacccc gctttcggtc gccgcaaacc gccagaggaa acgccgcaac
      841 agcaacgtct gcgccaccat ggcgacagcg ccaaaaaccg ctgccgtagc cagtatcgaa
      901 aatgaacgaa gcttcctcac ccttcatacc ctctatgccg tgaacattga gcgttcgttc
      961 cgcctttggg agggacgccc gcgctccaga ctccgcaccg catattcccc gccctcttca
     1021 cgattcacga ctcaaacatc ctcaaacttt tgtcccggct gcggcgccac cacgttctct
     1081 accccgcgcg cccggaccaa ctcgatcatg gcctggactt tttccggttc gccgtggacc
     1141 gcgaacaccc gcttcagacg cttgccgcgg accgcgtcga accaccgcat cagagccgta
     1201 cggtccgcat gcgcagacag tgcgttgatc gtgtggacgc gcgcgttcag ttcaaactca
     1261 tcccccagaa tctttaacgg cgaacggcgt tcgacgatcc ggcggcccag agtgttttcc
     1321 gcctgaaacc cgacgatcag aatgatgttg cgcggatcgc ccacgccctg tctgagatgg
     1381 tggagaatac gtccgccctc gcacatgccg gacgcggcga tgatgatcat cggccccggc
     1441 cggtcgttca acgctttcga ctgatcgggc gtctccacaa actgcaagcc cggaaagcgc
     1501 agcgggtcga tcccctgccg caacagatca gaggcctcct cgtcgtagca ctcacggtgc
     1561 cgggcataga cctgggtggc cttcagcgag agcggactgt ccacatacac cggcgtgagc
     1621 ggcatgcgcc gctgttcgat cagccggttc aactcgtaga gcagatgctg ggtacgtccc
     1681 acactgaacg ccggaatgat gagtctggct ttctgctggt ggatatcctc aacgaatcct
     1741 ttgagccgac ccgaaacgtc ctctcgcgca gggtggtccc ggtccgcata tgtgctttca
     1801 atcagaatcg tgtccgcgcc ctgcggaaaa tcgtaatcgc gcagaatcgg tcggtcgggc
     1861 tggcccagat cgccggtgaa caacagccgc cgcgacgtcc cgccgcgcgt cttcacatcc
     1921 aactgcacga gcgcagagcc gagaatgtgt ccggcgttgt gcaacaacaa ggagattccg
     1981 tcccccagat cgaccagccg tttgagcggt tccggttcga agcgggagag gatgtggtcc
     2041 acatcctcct gctcatacag cggctcgaac agaaccttgc cttgctgcgc acgttttttg
     2101 ttcacgtatt ccagatcgcg ctgttggagg tacgcggagt cgcgcagcat gatgtcgcac
     2161 aggtcgcgcg tggctggcgt gcagtacacc ttaccccgga acccgtgccg cgccagagtc
     2221 ggcaggttac cgctatgatc gatatgggcg tgcgacagga ccacggcgtc cagcgtccgg
     2281 ggatcgatcg gcatgttgcg gttcttttca aacgcctctt tccggtgtcc ttggaagaga
     2341 ccgcaatcca gcagaatccg cttcccgttc gcctccacca gatgcatcga acccgtggtc
     2401 gtttgcgccg cgccgaagaa tgtgagtttc atcatcccgc ttcctttaac tgatggttct
     2461 gatccgggac cagtatatac ccatcccgtc attcaatcca gcgcaacgcg agaataacag
     2521 gaacgccgaa cgccggtatg gcacgggcat cttgcccgtg tttcatgggc gggacaccca
     2581 tgccgcaaaa ttcttcgatc catgccccca aaaaatcttc gtgttcttcg cgtcttaccg
     2641 ccgtcatccc gcggccccag cgcaccaaac gtcccgcatc tctcatcacg caccctttca
     2701 acgcgggctt ctcccgcaac ctgatcggca caacctgttt ggaatcggca gggcgtacgg
     2761 tctcaggcgc agagttcaga acaggtcatc gtcctcctca tccgcaccgc cgaaaccgcc
     2821 atccccctct tcgtcgaaat ccgcgccgta cagcacatcc tcccccccgt cctcttcctc
     2881 ctcttcgaca gcgaagagcc gactgcgaag cgcaccggaa ccctcttccg tgacgttgaa
     2941 ccggcagtcg aactcatcgc aggcggaaca ggccctgggc gtgaacgccg cagccgaggt
     3001 ttcgccgtcc aacaccagcg gacaggttcg cgcggtcggc cccgcgaccg cgccggtcga
     3061 tggtgccgca ggcctctcga cggcaacggg cggacgcaca cccgcagcgg aaaccggttg
     3121 cggcgcggcc ttacggggtt ccggtttcgc ggcggcccgc gccgcagttg cagccgtttt
     3181 tttcccggcc ggtttcacag atgtccgttt gacggatcgt ttgctcgtcg ccatatcagg
     3241 aatcctgcct ttccgcgggt tcagccccgc aacagcgtca ggccttctcc acatgcaggc
     3301 cgaccgcgtg cccgttcaac tcgatcaccg gcccatgcac ttcatcgaac cggatgccct
     3361 cacacagcgt ctcggctttc acataatccg cgtactgttc gatcgcctcg cgcacctcgg
     3421 ccccgcacgc caccgtaacc cggatacgct gcgtcacctc gtaccccgcc tctttgcgca
     3481 ggttctgcac acggctgacc cactcacgcg ccaacccctc gcggatcaac tcaggcgtca
     3541 gcgcggtctc gatcccaacg atcacatcgt cctcagaggc cacgaccatc cccactttgg
     3601 gagtccgccg caccaccacg tccccggcac aaagggtcat cgattcgccc ccgaccgcaa
     3661 tcgaaacctc gcgccccgcg gcaagcgcgg cagcctcggc agaggaaaga gcggcgatgg
     3721 ccgccgcggc cgccttcatg cgagggccga cccggggacc cagctgacga aaatcggctt
     3781 tcaccgagac atcagccata acggtctcgt cctccccata tgccacggtt ttgatgttca
     3841 gctcatcggt gatcagatcc tcatacccct gcaggatcgc gcgaagaccg gcattcccgg
     3901 agaccacatg cagcaccgcg aggggctgcc gaatcttcag atcgttctcg gtacgcaact
     3961 ggcggccaag ccgcaccacc tgctgcaccc gggccatcgc gctttccaac gcctcatcac
     4021 ggcccgccgc atacgcctgc ggaaaatcgc acagatgcac cgactcgggc attgaggcgc
     4081 cgcgcagatt acggtatatc gcctcgctga tgaacggcgt gaacggtgcg gccacctgac
     4141 agagctgcac cagcacatac cgcaaggtcc ggtaggcgtg cgtcttgtct tcgtcgttct
     4201 gagatttcca gaagcggcgg cggctccgcc ggatatacca gttggtcaga tcctcaataa
     4261 acgtgacgaa cgggcgcacg gaacgctgca agtcgtatcc gtccatcgcc accgtcacat
     4321 cccggatcag cgtctcgagc gaactgacga tccagcggtc cagcacattc ggactctcgg
     4381 gacggaccac ctcggcatcg tcaaaccggt cgacgttcgc gtacgtgacg aaaaagctgt
     4441 aggcgttcca ccatgggatc agcaattcgc gcagaatctg ttttacgccg gtttcggaga
     4501 aacgcaagtt ctcggcgcgc accaccggcg aataaatcat gtagagccgc agcgcgtcgg
     4561 ccccgtacgc atcgagaatg tgcgacgggt ccgggtagtt cttgaggcgc ttggacatct
     4621 tcttgccgtc ctcagccaga accagaccgt tgacgatcac gttcttgaag ggcgactggt
     4681 cgaagagaca ggtccccagc accatcaggg tatagaacca gccgcgggtc tgatccaaac
     4741 cttccgcgat gaagtccgcc gggaagaatg tggagatgtc ctgcccgccc ttccctctct
     4801 tctcctcaac tcctaaatcc tcaactccta aatcctcact cctcactcct aaatccccga
     4861 aggggtaatg ctgctgcgcg tatggcatgg ctcccgactc gaaccagcag tcaagcacct
     4921 cgggcgtgcg ccggtagacc ttgccgtcgc gccggatcac aacgcggtca acgaaatgct
     4981 tgtgcaaatc cgtgacgcgt tctccggaaa gctgctccaa ttccgccacc gaacccacgc
     5041 agatctgatc cgcgggatca ttctcattga tccaaaccgg aatgcaggat ccccagaaac
     5101 ggttacggct gatgttccag tcgcgcgcct cgttcagcca gttgccgaac cgcttttcgc
     5161 cgacatactc cggcatccaa cggaccgtag cgttgttgcg cgtcagccgc tcatgcagat
     5221 cctcgacgcg cacgtaccag gcctcgatag cgcgatagat gagcggcgtg tccgtacggt
     5281 cgcagaaggg ataactgtgc gtcacagtag tctgatgcac caacttgcct tcacgcttga
     5341 gccactggat cagatccttg tcgcaatcct tgcagaaccg cccctgacac tcaggcaccg
     5401 cctcggtgaa acaacaagcg gcatccaatg gatccgcgat gacgctcaaa ccggcctgct
     5461 ggcagacccg gaaatcgtct tcgccataag ccggcgccat gtgcaccagt ccggttccgt
     5521 cctccgtgct cacgaagctg tcagtcagca cacggaacgc gcctgcgtcc cgatactccg
     5581 caaaatgagg gaagatcggc tcgtaggtcc aatcccgcaa cgcctcgccc ttgagacgcg
     5641 ccagcacctc gtacgcgccc gcatccttgt aatacaccgg aagccgggcc tccgccagca
     5701 caaagacggt ctcgtcctca cagtcgcgca ccaccacgta gtcgatctcc ggaccgacgc
     5761 agatcgccag attctcgggc agggtccacg gtgtggtcgt ccaaatcagc agataggtcg
     5821 taccccatgc gggctcggcc cccgaacgca cgcgcacacg aacggtcaca gcgggatcct
     5881 gcacgtcctt gtagttgttg cccgcctcga aattgctgag cggcgtgctg agtttccaac
     5941 tgtacggcat gatccggtag gatttataga tacgcccctg atcccagagc tgcttgaaca
     6001 cccaccagat gctctccatg aaaccggggt ccatggtttt gtaatcgttg tcgaaatcga
     6061 cccagcgccc catgcgcgta acggtcttgc gccactcttc gacatacgtg agcaccatgg
     6121 agcggcactg ctcgttaaag gcggcgacgc cgaactcccg aatcgcctgc gcgccgctga
     6181 cccccagagc ctcctgcgcc aaggcctcga tcggcagtcc gtggcaatcc caaccgaacc
     6241 ggcgttccac atggtggccg cgcatcgtct gataccgcgg cacgatgtcc ttgatcgtac
     6301 ccgccagcaa atgcccgtag tgcggcaaac cggtcgcaaa aggcggccca tcataaaaaa
     6361 cgtactcgcg ttgtccccgg cgctgctcca cgcttttccg gaaggtgtcg tgcgactccc
     6421 aaaacgccag catgtcacgt tccatgccgg gaaaatccgt ttgcttcgat acaggttcaa
     6481 acataagatt cactcatttc ccccttccgc tcaccccatg gcgcacgaaa ggtcaagatg
     6541 cgtaaaagca agataggaaa actataaagg aactgccttg acacggcaag accgtataag
     6601 aacttaacaa cctataccgt gtaacctgta acctgaaaac cgtccccgtc agccattagc
     6661 tattgcaaca cctttaaccc gctgaacgtc aacaagtaag cttttccgat tcaacggaaa
     6721 cccccacaga cacacggttt tcggcggatc gagaaagcgt aacatcctct gaactaaaac
     6781 gttgaaggcg ttacaccccc gtcgcctgtg cggtcccgct tccgtccaca tatcatgaga
     6841 agtggacagc cctgcacaga accagcggga accattcgag gtttgcgtta cggttttggt
     6901 ttctctgctc gtcgggtcat ggagttcacc tcattccacg cacccggcgt cttgtcatgc
     6961 ttgatgagga atgcctgcaa atccttcggc tgagctgtta agaggcgcct tcttcccttc
     7021 gtggtcaaca atccgcaaca tgtaattctt cccgtcaccc tctgtgaacg tccacttact
     7081 cttatccgta ttatccttgc ctctccactc gccaatcagc aaaggatcaa agatcagatc
     7141 ctgctccgtg tataacgggt gcagggatgg agcacacccc atcaaaaaac caagatcccg
     7201 agggtcatga tcgcagtcgt ctttttcatg ttttgccctt tcattcagac gcgccgcgcc
     7261 ggacccgcga ccgcgcgccg taaagcgagc cacagcacat ccagcagcat ccgccccccg
     7321 ctggagaaga cccgaaccgt cgaatgcccg ccgtaattcc aaatcaccgg aacctccaca
     7381 acccggtacc ccaaatcccg cgccatcgcg atgatctcga catcaaaggc gaagcggttg
     7441 atgcgcgaac ggctgaacac ctcgcgggcg acatcccgcc ggaacagttt gaacccgcat
     7501 tgcgtgtcgc gcatgtcgtg aaccccggca agacgcacaa gaaggttgaa aacccggctc
     7561 aacaccctgc ggtgcaacgg cggaggcgta agaatatgag cgccgcggac cgcccgcgac
     7621 ccacaggcaa tcgccgcgcc cgcatccacc gcctctttca accggaccca ctcttgcaac
     7681 ggtgtggaga ggtcggcgtc cgacatcagg atcagatcgc cctcggcagc gagcacaccg
     7741 gtccgaaccg cgtagccctt gccccggttg accttgtagc ccagcagccg gatccggtca
     7801 ctgcccgaag cggcgatgaa ctcgcgcacc acatccgaac accggtcggg tgaaccgtca
     7861 tccaccaata tcgcgtcaac cttcacctcg gtcgtgtcgc ggataaacgc cgcgacttgt
     7921 tccagcgtcc cctttaaccg acggtactcc cgataaaccg gtatgacgac tgttaaccgc
     7981 atgcctctct ttcccctttg agtgttatcc ctcgtttgtt cgcaacgcat tccgcacagc
     8041 ggacggatcc cgcctttccc tcacccgcct gtaagtagcg cgaacgcctc gtgagcggtt
     8101 ttcgcggcca acaacccgtc caacgtctga ccggtcaagc gttgccgcaa ctcggcttcc
     8161 acctcagcgt agacagtggc ctcggattcc ggatacaaca ccatcaccca cacgcgcgga
     8221 gatgttccgg agccagatgt cgcggaagcg attcccaacg ccgcgaccat gcggtcgaca
     8281 tgcgccgtcc gtaccggaaa taccgcgacc tcctcaccga tccggaccgg cggcgtctcc
     8341 acgccccggc ggatatccgc catcgcctcc ggcaacgcat tgaccgcccc gctgcgcgcg
     8401 cagagcgcca acagcagggc aagaccctgt tgacgatcct ccacatcgaa atcgagggcg
     8461 atgctctgcc aacggaggca acgcgccaga atcgtctgcc gtgcgcaaaa cgccgcgccg
     8521 gcgcggccgc cggtgagcac cgcatacatg tcgtccggcg tgtcgcacgc cagcagggcg
     8581 gcgcggccgt ggctgtccag cagccgacac aggtgcgcga tcacctgcaa ttgcggcgac
     8641 gccgcctgtt cgggagccag caccagcgcc acgatacggg ctgggcggcc gtccacggtg
     8701 tcatatgcca aaccttcttt tttcaggccg accgcacaca ccagacgcgt cacggcatcg
     8761 gtgcgggcgt gcggcagggc cagcccgtgc tccaaccccg tggagagatt ctgctcccga
     8821 tccaaaaccg cctgcaacgc gcgatcccgg tctgtgacca gtccgctctc gcataacagg
     8881 ttgacgagtt cggcgaccgc cccggccgga gtggaggcca gcaaccgcgg acggcaggtc
     8941 tcggggaaca ggtggttccg caacacaccc gcgctcccgg ccgcagacgc ttccggcacc
     9001 tgtatgtttt ttctgagcaa cacgtcatcc agcggtctcc gcaacgcaca aaggttcccc
     9061 tcgatatcgg aaagcgcttc cagcatcact acattcacca gcgcgcgctc gcgttcgccg
     9121 cattcgaacg tgatctcccc gtcatggctc tggagatgga tcacctgagt ctcgcggctg
     9181 atgcggtaga gcatgtgatg ccggtttaac acctgcgcgt aaaagccctc atcctcaaac
     9241 agctcaacaa tccggttcgc cataagctgc gccgcctgcc gcgaagggaa ccggaacacc
     9301 agccgcgccg gctccggccc gcttccaccg caaccatccc cctgatccaa aacccggtca
     9361 gagaggaacg gcaaagccac gcaggagcag agaatcagaa acacggcgca aaacagcagg
     9421 ctttggggca acgggatcat gccgccgacc gccgccagca atgccaccga aagttcagcg
     9481 cgtggcagtg ccagaatccc cacgcgcagc gatgtcgcga cgcctgaccg tgtcagcgac
     9541 gccccggccg cgcatccgat cagattggcc cccaacaccg ccacagccaa cagcaccgac
     9601 gtgccgagca cggtctcgtt cgccagaaga ttcggattga gacgcatgcc caacgaggcg
     9661 aaacaggccg gcatcagcgt cagactcacg aaatcgaggc gctcgcggat ctcatgccgg
     9721 acatccatcg cggagaaggc cacacccatc atatacgcgc cggagacgac cggcagcccg
     9781 caggccccga tcaacccgcc cgcgatcagc agaccggccg cagagaccgc aaacggctga
     9841 ccgtaattct tctcccgcac ggctaacgcg ttcatgcggc gcgcaagcgg gatgcccaac
     9901 aacagcacag cccccaaccc gacacccgcg cgccatgcca ccgccgcaat ccgcgcaccg
     9961 gaaagctcgt tcactccgcc tcccgcgccg ctgacacccg caaccacggt gaacacgatc
    10021 aacccaagca cattcccggc catggccgct gtcagcgaca gggtgcccac cggcgattcc
    10081 aaacgtttgc ggaccgccag cagacgggcc agaaagccga cggatgtgac ggaaatcggg
    10141 acagcgcaaa gcaacagcgc ggatgtgtgc cacggctgcg ccggacccag cagcgttggc
    10201 gcggcccgga aaagcagcgc gagccccgcg ccgaacgcaa acaggaatcc gcacacaccc
    10261 gccagcaccc cgctcgccga caggcgccgc atctgacgca agtccgtatc gagtccggcc
    10321 agaaaaaaga agaccgcgag gatcacggcc atcagaccct gcccgccccc accccacacc
    10381 gacaggaacg cctccgcatc ccccatcaat ccgtgcggga aaaacggcaa cgccaccccg
    10441 ccgagcagat acggccccac caccaccccg gcggccacct cgccgaacac catcgggaga
    10501 cgcccccgct caaacagggc ccctcccagt ctggccgcaa acaccaacac cccgagttgc
    10561 agaataaaac gtgccgcggc atccgttgct gatcccgacg caccgcaacc gtcagcccag
    10621 accggagaca cgggtatcgc ggccaccgcc agtgccgccg ccgcccgcct cattccgcgt
    10681 gtgatcattg ccgcctccgt ccgtggttat gcgccctgcc ggttgatccg cgcagcggca
    10741 acccccgcgc cgaatccgtt gtcgatcttc accaccgtga ccccggccgc gcaggagttg
    10801 agcataccga gcagcgcggc aagcccttgg aaactcgccc cgtacccgac gctcgtcggc
    10861 accgcaatca ccggacggtc cgtgagtccg ccgaccacgg aaggcagcgc gccctccatc
    10921 ccggccacca ccacaatcgc gcgcgcgtcc tcgaaccggt cgatccgtga gagcaaccgg
    10981 tgcaggccgg ccactcccac atcataaacc cgttcgatcc gcgcgcccag acgctcggcc
    11041 gtgagtgccg cctcctccgc caccagaaga tcggacgtgc cggcgcagag taccgccacc
    11101 ttgccttccg gttgcggcaa cggcgcgaca tcacgggtca agatgtgcgc ctcttcgcgg
    11161 tagacggcat cagggatctc cgtttgaaca gcggcagcct gctccggcgt cacacgcgtc
    11221 acgaatgcgt tttgtccggc atcgttcaac gcgcgcatga tcgcggcgat ctgcacggca
    11281 gtcttgcccg atccgaaaac cacctcgggc atccccaacc gccgctggcg gtcgaaatcc
    11341 ggttttgcga agtgaagatc ttcagtcata gattacacca acaaggcatc gtccagtttg
    11401 gctttgacct gctcgatcgt aaggcgttcc tgcagcatgg tgtcccggtc gcggaccgtg
    11461 accgtgccgt cctcgatggt ctggaagtcg atggtcacgc accagggcgt gccgatctcg
    11521 tcctgacggc ggtaacggcg gccgatcgcg ccgccctcgt cccagaacgc ggagtagcgg
    11581 cggcgcaggt cttcgaaaat cccgcgggcc ctgttgtaga gatccggctt gttcttcacg
    11641 agcgggaaga cggcgacctt aaccggcgcg atgcgcggac tgaaatgcat cacgatacgg
    11701 gtatcctctt tgcccttatc gtcggtcaac ttctgctcct cgtaggcgtt gcagagcagc
    11761 gcgagcatca tgcggtccac cccggcagaa ggctcgatga catgcggcac gtacttgccc
    11821 aagaagagct tcgacacgaa ggcgcgcgcg tcggactcgt ccttaccgtg cgcgcgccac
    11881 tcggcgacga ccttatcggt gaacgcgagc ttagcggcgt catccagctt atcggccgcg
    11941 agcttgagcg gctcgtcaaa cacctccatg cttttgccgc tgtgcttttg gtgctgcgag
    12001 agatcaaaat ccgagcgcgc cgcgatccct tccagctcct gcacgccgaa ggggaaccgg
    12061 tattcgatat cgacacaggc gcgcgcgtag tgggccagct cctcaggctt ctgccagtac
    12121 tcgacaaagg acgtcttggg cagtccgcac gcctcgagcc aagccttgcg ctgctcgacc
    12181 caatatttgt gccacacctc ccagccccac gagtcctgcg gcacggagag atcgggatgg
    12241 tcgtccaagg tggtgacgtg tccgcaaatc agctcgaccg cctcgtcggg ccggatgaag
    12301 aactccagct ccatctgctc gaactcgcgc gagcggaagg tgtagttgcg cggcgtgacc
    12361 tcgttgcgga aggacttgcc catctgcgcg ataccgaacg gcacctgctg gcggctcgtg
    12421 gccagcacat tctggaactg cgcgaaaatc gcctgcgcag tctcgggacg cagataggcg
    12481 atgttcgcgg aatcgtccac cgggccgacc acggtcctga acatcaggtt gaacgggcgc
    12541 ggctcggtca actcaccgcc gcagtaggga caggggacca ccgcaggctg cggggccttg
    12601 gcattcgcca gcagcgtgta gagcgcgcgc accgcggcat atggcgcggg atcggccttg
    12661 agcgtctcat aggcggcggt cagcgtgtcc gcatcgagca cggccgccaa attcggggca
    12721 agccctttgg ccttgccttt gcaccagccc gcgacttggg ccgcgtccgc gccgccctcc
    12781 agcaacgcct tcagactctg gccgatcgcg ccttccgcca gaatgtccca cacctggtcg
    12841 gcgcgcatca gctttttgca ggcccggcag gtgcacatgg ggtccgaaaa ggtatcaagg
    12901 tgtcccgagg ctttccagat cgccggatgc atgatgatcg tggcatcgag tcccgccaca
    12961 tcctcgcgat tgcgcaccgt ggtcttccac cagtcctcct tgatgttgcg cttcatctcg
    13021 cagcctagcg gcccgtaatc ccaaaatccg ttgatccccc cgtacacctc ggaactgtga
    13081 aaaatgaaac cgcggcgttt acacaatgag gcgatgacat ccaagctgct tgtgttcgac
    13141 atatccttcc ttcacttgcg tggtcgcgca atcatgcgct tatgaaattg ccattctcgc
    13201 cccccgccgg tcagggacca gtgcaacacc ggcccctgac cggcgggctc gcgaatcctc
    13261 caggtgacaa accagtgtta gtttggcatg cggacggggt gtaggcaagg ggaaacaggc
    13321 cgtgaacaac gcgcttctcc gtccgatgat ccggataatc ccgttattgt cccttccaac
    13381 ctgccgtcac acttcatacc ccagaggaca tgagacggca accggtcacg aacgggacac
    13441 acgatgatga tgcaccgcgt gagcggcaaa gtacggaacg tcaccggcct tgggcaggtc
    13501 agataaggcg ctttttacca ccccgtcgcc tttgtccgct tgccagaacg atggcatttt
    13561 gcgacactgt cgtcaaacaa cacgttcacc ttgggaggca atcacatgat gcggatcaca
    13621 cgatatgtcc tatgtgcggg gatcttttct gcggcctgct gccttgcggc agaggaaggc
    13681 tggaccccgt tgttcaacgg gaaagatctg gacggctgga tcccgaaaat caaagggtat
    13741 cccgcaggcg agaatttcgc caacaccttc cgcgtggagg acggcatgat caaagtgcgc
    13801 tacgacgggt acgacggcgc gttcaaaaac cgtttcgggc acctttttta caaaaccccg
    13861 ttctcccact atattctgcg tgtcgaatac cggtttgtag gagagcagtt gccggacggt
    13921 gccggatggg ccaaacgtaa cagcggcgtg atgatccacg gacagacgcc ggaaagcatg
    13981 ggagtgaacc agtcgttccc agtatcaatc gaggtccagt tcctgggcgg actgggcagc
    14041 ggcaaccgca ctaccgccaa cctctgcact cccggcacaa atgttgaaaa ggacggcaaa
    14101 ctttacacgc cgcacggact cagcgccaaa tccaagacct atcacggcga ccaatgggtg
    14161 acggttgaga tcgaggcgcg cggtagcgaa accatcctcc atcgcatcga gggcgagacg
    14221 gtcttgcagt atgacaagcc gcagtatgac cccaaagacg gcgatgcccg acgcctgatc
    14281 gaggcgaacg gcggcaagct cctgatcgag ggcgggacaa tctcgcttca gtcggagagc
    14341 catcccatcg acttccgtaa aatcgagatc aaggtgctga agccgtagtg tccccggcac
    14401 catggcgccg ggccgattcc tgagccatcg ctgactccgc aaacagcgct tccacgcgcc
    14461 gcaacagttc ttcgcgggtt tcgacgcgca gcaatgggca cggcagactc ttgcggaagg
    14521 tggcgccgta cgatttggtc accacacgcg gatccgcgat gatgaccacg ccccggtccg
    14581 cccgggtgcg gatcaggcgg ccgaaaccct gccgaaagcg gatcacggcc tgcggcagcg
    14641 aaaactctcg gaaagcgcag cctcccgcat cttcaatctg ttcacagcgc gcttcgacaa
    14701 tcggatcggt gacggccgca aagggcagcc gcgccatgac cacgcagctc agcgcctccc
    14761 ccgccacatc gaccccctcc cagaacgaat gcgttccaag cagaacgcag ttcccgccgg
    14821 tacggaagat ccgtgtgatc tgatcgcgcg acccgttggt gccgtgcacc agcagacgga
    14881 tgccggcctc ctgcagcggc tcctccagca gccgcgcgac ccggttcatc atttcataac
    14941 tggtgaacag ccccatcgcc cgcccgcgtg tccgcgtgaa cacgtccaac atcagaccgc
    15001 tgagttgctc ggcataggtg ccgcccgtgc cccctgtggg ttcgggaaga aaaaccggtg
    15061 ccagcgccgc gcactgcgtg agataatcga acggactcgc cgccacacag gtcaggagcc
    15121 gatccggttc gagccggtcg atccccaatc gccggctgat atacccgaac gaatttccca
    15181 cgcgcaacgt ggccgaacag aacaccacgg tggccttccg gcggtaaagc gtctcggcca
    15241 gcgcttcacc gatacgaagc ggtgccgcgt aaagatgggc agtccccgca ctgcggctcg
    15301 gcggttcggc ccaaaaaacg tgttccgcgt catacgcggc gagaacgaaa tccagatcca
    15361 tcgcataggt ccggagcgac gctgccgcgc cttcgacatt ggcagcctga tcgtcataca
    15421 gcgcgagctc gccttccgtc gcctgccgca aggcttcccc caaggccagc aacagaccgg
    15481 aggcttcggc gatcgcattc ttcaacccga tgcgatgcat ttggacagtg tcctcatccc
    15541 atgcgtccgg acagggcgtg aaccgcttat cacggaaaac ctcgcggcgg aaaccggagg
    15601 gaaacacgcc gccgcatgcg ggttgcccgg catttcccgc cgcgagggcc gactcccccg
    15661 tcggccaaac tgtcgcgtca ggaccgtcgc agggcacaca ccggaaacgc accggaccgg
    15721 gttccggctg gatcaagccg tggagcgact cgaacagttg ccgagccgca gcctgcacct
    15781 cttcgagcac attgcggatc tcccggattt gacgccgcag cgcttgggcg tattccggat
    15841 ccgcagtcac cgcgcccttc tccaaatggt tgcgcagaac ttccagcata cccccggccc
    15901 gccgcccccg gccccggctt agtcggcgca acagttgggt gaggcgcgtc tgcgtaatct
    15961 ccgtggagag gtggcgcgtc gcggcctcct ccagattgtg ggcctcgtca aacacgatct
    16021 gggcatgcgg cggcagggcg gtcccggggg cccgcgactc cgcgaacacc agagcgtgat
    16081 tcgcgatcac cacatgcgcc gcagcggcgc gcgcgcgggc cctctgcaaa aaacagcgcc
    16141 gactgtagcg gcaggcacgg cctggacact cttcggcggt agaggccagc ttcgcgagga
    16201 actcggcatc gacacgtccc ggacccgcaa aggtatcaag atcgccgtcc ggcgtctgcg
    16261 cggcccaggc aagcacatac gcgaaatggc gccattccgg gcgatcgagt tcgtactggc
    16321 tctgctccag cagaatcgcg agccgccgca gacagaagta gtttgagcgc cctttaacca
    16381 ccgccacgcg cagagcggcg cgcccgccag gacagaccgc ctcgcggacc cgcggcaaat
    16441 cgttctcgat cagttgggcc tgcaagttcc gcgtgttggt gctgaccacc accggaacat
    16501 cgttgagcag cgcccaggcc gcggccggaa tcaggtaggc cagacttttg ccgacacccg
    16561 taccggcctc gaccaacaaa tggcgcccct cgttgaaggc acgcgtgacg gcgtgcagca
    16621 tctcgacctg tccggcacgc ggctcataac cgggcatgag acgtgcgaac gcgccgccgg
    16681 gaagcagatg cgcggccatg gccgcaacat ccagcggcga gcagtcggca tgctcgggca
    16741 aggcacggcg atccccccgg cgcgcctccg gggcgaaggt ctcgatccaa ggcccgcatc
    16801 cgacgccagc ttgacgaacg caagccgcga agtggctgaa gacgcgcgcc agtccgtgct
    16861 catcgagatt gcgcaggatc ccctcaatcg actccagtgt ccacaggggc aacgcgttca
    16921 aacgcgccgc cacagcctgc cacagcaccc cgatcgcctc accggtgtgc ggcccctggc
    16981 gtatttgcgt ggtcacctgc tcggcgggcg tgcccgggaa ggcgaaggcg tacagaacca
    17041 gcacatcgac cgcccacgcc ccttcctgcg gcaagacccc ctcaaacgcg gccggatcct
    17101 gcgtgacgta caccgtctcc ccgccgtcac gttgatccgt gacgggaaag agatcgaaga
    17161 ccggcggtcg gccgggatgt cgcgtgaccc aaactgtacg gaagttcagc atgtgcgccg
    17221 aaccggaaat ccggcctcac ttccccatgt cgaagagctt caggcaatgc cggaaatcgg
    17281 cgggcagcgg gctgtgcgcc tgaagggttt tgcccggccg catcggatgc ggcaacccga
    17341 gtcccgatgc gtggagcatc aggcgcggca cactcaacaa acgcgggtcc cgcgcaactt
    17401 tcaccccgta tttgcggtcc cccagtacgg gatgacgaat cgcggccaga tgacgccgga
    17461 tctgatgggt acgtcccgtt tcgatccgca cctttagaaa actcgcgtcg cgggagagca
    17521 tgatccgctg aatgtggcta accgcgcgct cgccgtccaa ctcctgatct accgttgagg
    17581 cgtgccgttc gtagcgcccg gaaacgatcg tatgatactg cttggtcacc cgccgcgtct
    17641 tgaacaactc cacggcggcg tcaaaggcct ccggcgtctt ggcgcacaac agacagccgg
    17701 tggtgtcccg gtcgagccgg tgcaccgcct tcagttccgc gatccccgtc tgcgcgcgca
    17761 gccgctcctc cacgctcccc gcgccgaccg tcagcagacc ggaaggcttg tccacaaccg
    17821 catattccgc atcctcgatg agcaggcgga ttttcggcac cgcaacgggg ttcgcgtccc
    17881 gcgcgccctt acgcagcacc gcagccggca actctaccgt atcaccctga cgtagcgtat
    17941 gatgcgccat ccagacacat ttgcggttca cccacaccac ccgttcgtcc agcagggctt
    18001 tcaccgcccg tcgggaacgc gagagtttcg cggcgagaaa aacctgaacc gattgacctg
    18061 cctcgctctt ggacaccaca aaagacaaca cacccgcccg gctcatatca cccgctcttt
    18121 ccgcttccgc ccccgttaca cctgcagaag cgaacccacc acccctgcca ccgcatcccg
    18181 tcccaaacgg ttcggaccca tctgaacctc aagatcgatc cgcttgtctt cctccgcgtc
    18241 tgccaccacc cggcaggcca ccggttcgcc acccagcgcg cgattgaaaa gctccggcaa
    18301 cggctcgtcc ggcattagat cctcaaacgg ccgtcccaac acctcgttct cttcccgaag
    18361 atcgaacatc ttcaggaagg ccgggttgac acaccggaac acccggtccc gatcgcacac
    18421 aaaacaggcg ctctgcgcgt tctcaaacgc attcgccatg ctgcgcagag cctgccactg
    18481 tttgcgccgg cgctccgtgt tgcggaccgt gaacaccaaa tcaccgctat tgatcagatc
    18541 aatcacgctg atggtcacct ccgccgcgaa caccgttccg tcctgacgct ggcacttggc
    18601 gtcgagcatg atgtggcgcg cctcatcgat ccctttgcgg atccgcccga tcatctgcgg
    18661 ggtgacgccc gggatcagaa aagccaccgg cttgtcccac acctcctccg gcttaacctg
    18721 aaaatactcg gtcgcacgcg gattgatctc aatcacatgc ccgttcgggt ccgtgatcag
    18781 caacgcatca tacatgccgg cgatgagttg acggtaaagt gatttatgat cccgcatcgc
    18841 gcaaacggat gtcatgtatc ccccctcttt tccaacacct gccgcacacg gttacatcgt
    18901 gcgggacacg ttcacaacaa catgattact atgacaggtt acttcgcttt ccgcaattcc
    18961 tgttttcatg aaatatggac tcgccttacc ctgtccggcg tattatcatg atctcaccat
    19021 ttggggaggg agttatggaa gagcggcttg acgaaaccac cttcgtttca ttcatttgtg
    19081 aggcgtgcaa tcaggagatt gaggcacccg cagaccttgc gggccgtgaa accgagtgcc
    19141 cgacctgcgg cgtgaccctc acggtgcctt acgtctccac gcccggaacc ctctggggcg
    19201 gtccccttga aaacacgggg ggccccactc ccgcgcagac ggaggccatg aagagccgga
    19261 cgatccgcat cgaactgccg gacagttggt gatcgtccca tgtcccgcat cctgttcatc
    19321 tccccgaaac cctactcccc ttgggaaggc gcctgccacc ggacgcgcca tatactcgaa
    19381 gcccaaattg ccttagggta cgatgtcgac ctgctcaccc cgacggaggg tcagacgccc
    19441 accgttgccg actccgtcac cactatcccg ctgccacgcc tcccgttctg ccgacgcacg
    19501 cccaccctcc cttcgcttct ccgcctcaca ctggacctgc tcgcgcttct caagatcctc
    19561 tggcttgcgg gaaacacgcg gtatgcgctg atccacggac agggcaacag cggggtcctc
    19621 gcctgggccg cggcgcgcct gctgcgcatc ccctgtatcg tggaactgcg gaacgaccct
    19681 gcgccagacc ccgcgcaccg gcgcggcctc atccccgcac tctaccgacg gctggaacac
    19741 aaggcgctga gacaggcgga tgcggtcatc ggccatgatg cggcaacggt cgcgcgtctg
    19801 acagacttgg gctgccatag ccgcgcgtgc gtgatcgccg acatcccggc cgtaaccgag
    19861 gtggcggacg caccggcccg caatctggcg aaagcgcgtt accggaccct gaccgatcag
    19921 gatctggtca cctgcgtcgc ttcctatacc cggtttcagg gactcgacct ctttttcaac
    19981 gcgctcccgc gcctgttggc aacgctgccc cacacgcggt gcgtggtcgt cggcggcggc
    20041 ccggaggaga tcgcgcagat gcgcgaagcc ctcgaccggg caggcatcca tgaggccgtg
    20101 actttcccgg gccgattgtc caccagcgag ttggccgcgt tgctcgcgat ttcggacgtg
    20161 ctggtctcgt cccgccgtgc cggcgccacc gcacccatca aagttctcga ctacctcctt
    20221 tccggcactc ccatcgtggc agtcgacacc cccgccaacc gcgccatcct ctcgccggaa
    20281 aacgccgtga tcacccccgc cacgccggag ggattggcgg acggactcat ccgcctcctg
    20341 aggtcgcccg gactccgtac ggaactcaga ctcaaaggcc gcgaaaccct gcgccgcgaa
    20401 aaccgcacgc ccgaggcctt ccgcgcagcc ctggcgcgct gttacaccta tgcgctctcc
    20461 cggtcggagg ccgagtgaga tgacgcgccg cctcctccgt ttcgcgcgct ccttcgtcat
    20521 actggtctcg ctgtttctgc tgtcgacatt gatcgccgcc aacgcgctcg ttcatctgcc
    20581 gcgccaacgc caacagagcc tgctccaaaa ccttccggat gttgcggccc aaacgctcac
    20641 gcgtgtcggc catgccgcgg ccgacatcac cgacggactc ggctggaccg gccgtgacgc
    20701 cgtggccgcc ctgccgcagc cgacggacac caaccgcctg acccgcgcgg gtgtaccgcg
    20761 ccgactgccc tcctgcaccg cgccggatga tctggtcgtg ctcaacaaga tcggcttcac
    20821 catcggttat tcacccgcgt ggcgtcatcc ggtctgggtc gcctaccggg ccgacccgac
    20881 gtgccgaaac ccgctgttac cgcgcccctc cggattcaag cccgaccctg ccgcgccgcg
    20941 ttcgcccaaa caccgggagt actcccgcag cggatacaac cgcggccata tggttcccaa
    21001 ccgcgtgatc gcctcgcgtc acggtcccgc agcgcaggcg cagaccttcc tcacctcgaa
    21061 catctgcccg cagcgcgcct ccctcaatca ggggccgtgg gaaaacctgg agttccgtat
    21121 cgcggaactc tggcctacgc ggtacggttc ggtctgggtt ctggccggcg cggtgcccgc
    21181 acccgaggcc cggcgactgc cctccggtat ccatgtgccc gccgcgtttt accagatcgt
    21241 cgtgacccag ccgcaggacg ggacactccg cgccttcgcg gtgtacatgc cgcaacacgt
    21301 ccggcgccgc accccggtcc gctccatgct ggtttcgatc gacgagttgg agacgctcac
    21361 ggggttcgat tttctctccg agctgccgga cgaggtggag gcagaactcg aagccgccac
    21421 gcccacgcgc ctctggcccg tggggcttgt cggcaccatc cggcttgtgc gtgaacgctt
    21481 ccgccgctac cccgtgaaac gcgttcaggg cgcgcgctga tagacgcgca cataatcgat
    21541 ctcatacgtc tgaggcaaca tggcgtcgtc gatccccttt tgcccgcccc acgccccgcc
    21601 gatcgccaga ttgatgagca ggtagtgcgg tttacggaac gggttgtcct ccccggcacc
    21661 cgccttgtcg agcaagaacc ggtgatagag cgtatcgtca tagtagaaat ccatgcgatc
    21721 ctcgaaccgc tccacaacat agaggtgaaa gtcatccgac ggtttctggt catgcagttt
    21781 gccgcccaaa gaggtgcgct tgccttgggt cgtgtcaaac cagtgcacat tggcgtgaac
    21841 cgttgcgggc gcatgcccca cgaactccat gatgtcgatc tcgccgcaac gcggccagcc
    21901 gatggaccgg aatgaggtgc cgagcatcca gatcgcgggc cacatgccgc gtccgcgcgg
    21961 cagcttcgca cgcacctcga cacgcccgta tgtcaccgcg aacaacccct cggtggtcag
    22021 actggccgaa gtgtaggccg cggtttccgc ctcgcgccaa tcctcggatc cggacttgtg
    22081 cccgggattg gcaaacgtct ccttgcggcc ttcgatcacc agacacccgt tctcgacccg
    22141 cgcgttctcc cggcgcgcca cggtgtagaa ctgcgcctcc cgattacgga caaacccctt
    22201 ctcatacgtc cacttggccg gatccggcat gccgggccgg tcaaactcat ccgcccacac
    22261 cagccgccac ccctcgcgct ccgccgcgcc cgcaccgcat accagcaagg cgatcacacc
    22321 caacacgcat cccatccgca tccgctcccc ctttccgccg ccccacagcg gcatttcacc
    22381 tcacgttccg acacccaatc tgttacggca tgccattcac ggccaacgcc ttgccttggg
    22441 cagcctcgtc aagccggggc tccaccgcgc gttctgttat ccgttcacgg ttctgccgtc
    22501 gcgcgtccga tccggtccgc atcgtccgca tagctgtacg cgaaacgttg ccgcgtctcc
    22561 acgcaggtca gggtcggcgg cgtttgctgt ccgccggcct ggcgcggcag atactccacg
    22621 gcacaggctt ggacaggccg gtcccgggaa tcggcctgaa gcaccttgtg ccccagatac
    22681 gtgaaccgtt ccggcgtcct gcccggacgc gtgcggtacc agccggtgag acggccctga
    22741 tcatcgtatg cgtacagatc cttccaccct ttttcaaagg tgagcatcgg gtcggcgtac
    22801 cggtgctccg cattggtgta gtcaaccgac aggacctgtc cgtcatcccg ataggcccgc
    22861 acctcattcg gcagataata gaacgagatg atcgaaggcg cggtatatcc cttgccgtga
    22921 cgcgcgaagc atccgatatc aacgcggtgg cgcagcagag ccgagggcat cccttggtca
    22981 tcaaccggcc tgaaggcgcc gtgatacgac accgtgagtt tcacacgcga ggcgtttgcg
    23041 ttcagaggcg tgatgacaat tttccgggtg tcgccctgca acacggtcca gcggaaggcc
    23101 ccttcctgct gctgaccggg cactgcggcc tccacggtca tcacccgctc gcgggacacg
    23161 ccgcgcacaa cccgcgcgat gcagcagggc gtatcgaaca gcccctcgga gcgcaggtcg
    23221 aagaaatcca aacccgcctg aaccttggca tcctccactg tccgaagcgc caccaagggc
    23281 gggatcgcct ccggcgtcat ggcctgcgcc atccgcacca tggcctcgac atccagttgc
    23341 gcggcatcga atacaaccgg atgcgccgca ccggtcaagt accgctcctc gtcctttgcg
    23401 accgccttct gcgtgatccg gagaatccgc tgcagcgtcg gaccgatccg attccgctgc
    23461 gtcagcgcgc gcttggtcgc tggcgtgaac gccgccaagg ttgcggcgaa ggcgttcacg
    23521 aacggctggt ccgaacccga cgatccttgg ctgatcacga aaaacggggc gttgaccgga
    23581 tagagatccc cggtttcggg atcatggtcc cgatgttcag ggaacaccca aatctggttt
    23641 ccgagataaa aacgcaggga gatgatcgcc cggagcggat cggtcacggc cgcgcgcggc
    23701 agactgcgcc aatacgggcc actcaccatg gagagtgacg cgttgcccag taccggcgcg
    23761 tcgaacaggc cgttcggcag actcaggtgg acctgggcct gctttgcctg ctcgccatac
    23821 accaccggcg tcaggccggg atagttcgac accaccagca gcgaatgatt cccgtcccgg
    23881 ttcacataca gatcgccgaa attcccggac gccgtccccg ccttgaaccc cgcgcgcacc
    23941 gtttccgccg ccgcgccgcg atacgcctcc gcataatcgg aaacgggacg cagatccgga
    24001 acgagcaggt taaaccgtgt ccggaaacat ccgaactcga aatcccatat cgtgttttcc
    24061 gcattcactt ccgccggacg tcccataaca acggatacgg gacggacgga cggcatacct
    24121 tcaaccggtt ttccctgaag acgccgcgcc ttgtccaaca gttcccggaa tgccttgatg
    24181 gggccaagcg atttcaggtc ccggtccgcc tcaatcgcgg cggcgtcgcg gaaaccaagg
    24241 tcgatcgcgc gatccagcgc cgccaacgct tcccctttgt cggcacggta agcgagtgcg
    24301 caggccagat tgtactgcca cgtcgggtca cgaggcagca gggccacgcc ctcacggcag
    24361 atccgctcca tctcctcagt ctctcccgtc cgcacgaccc gcatcagttg ctgttgcaga
    24421 gcgaggtgtt tcggatagag caacggcatc tcaaacacgt tggtcacggc acccgcaacc
    24481 atcgaaatca gacacgcgca gacaaaaatc ccgcgtttcc tcatcacgtc ccccttccct
    24541 atgctgtccc gcactgtcct tttatccaaa cagcctacgc caccacggca ccggcgcccg
    24601 cgtctgccgc atcagccatt ggatgacctc gtcattcccg taggcgcgat cccacacggc
    24661 atgacccgca ccttcgaatt ccgtataccg gaccgcacgg ttgccgacct gtttcagcgc
    24721 ctgaaccata cgccgggaac aggccacatc gacattctgg tcggccgtgc cgtgaaaaac
    24781 ccagatcggc atccgccgga gttcccgcac ccgccggata tcccccccgc cgcaaacggg
    24841 aatcgcggcg gcaaaacgtt cggggcggcg ctgaatcgca tcccaagtgc cgaacccgcc
    24901 caacgaaagt cccgtgatgt agacccggtc cgggtccgcc tgacacgcat ccaccagatg
    24961 gtccaccatg gccagcacga cctccatgga cggggcaggc tgcggcggca tggcatagtc
    25021 ttccccgcgc gccagccgtt tcacccacca gttgccggtt tggcactgcg gcgccaagat
    25081 catcacaggt tcaggctggc ggagcagggt ggacagcagg gcgggcagcc cgactttgag
    25141 gtgacggcgg ttgtccgtgc cgcactctcc ggatccgtgc agaaacagga tcaggggata
    25201 tcggacgccg ggccggggaa actgcggcgc gctcaaccgg taagcgaacg tctccccggt
    25261 cggcgcattg gtaaaaacgc aggaatgaaa acgttcctcg accgtgaact gcgcacacgc
    25321 cttgagggtc agcgcaaaaa gaaccagaag ccagcctttc atggggatca gtatgccata
    25381 acccgtccgt cttggggagc gtcaattcgc gcgctcgttt cccgccggag cgtcatgcgc
    25441 ggcaccgctt tctggtctgt ccgcgccaaa gcggcgcgct gatcgatcaa ctcgccccca
    25501 gccctctgct catcaccacg cgcgccagcg cctcgatcgc cccttccgcg gtcgcctccg
    25561 ccgcgaccgc atccggttga cggccgaatc cggccagcgc ctccgaagtc ggcctgccga
    25621 tcgtcaccac gatctgaccg gccagcgcct ccacgcccgc ctgccccata aacgcctcaa
    25681 ccgctgatgc gctcgcaaag aaaaccgcat cggacgcggg caaccgggga tagtgaaccg
    25741 gttcgttgac gtacaacaga acgtcctcta ccgccgcgcc ctcttcgcgc agaacatcgg
    25801 ccaacagcgg tccggccttt tccgaacgca accgcaacac gttgcatccc gctatttcca
    25861 acgtccgcat ttccgccgcc aatcccgccg cactgtattc catcggcggc gtcagatccg
    25921 gatggatacc atggtgccga aacgccgcgg cactccccgg cccgcaggcc atcacccgcg
    25981 gcagacgccg caggtcgacc cccgcctgcc ggatcatgcc catgaacaga tgaaccgccg
    26041 agggtgaagt caacaccacc cagtcaaacg acgcgatccg cgtcaacgcc cgctccgctt
    26101 caacacacgg cgtcagccgg atcagtggcc ggagcaacgg cactcccccg aaatcgacca
    26161 cccgcaacgc ggtcttgtcc agcaacgcct cactgcacgt caacaacacg cggcaaccac
    26221 gcaacgcgcc ggtgtcccgc cgatacgcgc ccgccgccgc ttcaccgatt accaggagtc
    26281 ccggcgcctt cgtctccacc ggcgcggccg caccctcgcg caacccggcc agcgtggtgc
    26341 ggaaaatcag ttcgtcctcc gcccccgcgt caaaaaccag tccgaccggg gttttcccat
    26401 cccagccctc cgcaagcaac tggccggcca tctccgtcgc cacacgcacg gacatgaaca
    26461 gcaccaacgg cagacgccga cgcgcttcga gcgacacgct ggctacggcg ccgccctcga
    26521 cccgcggcgt gaatgccgtg aacccgcggg acacgccgcg tttggtgagc agcattcctg
    26581 tgccggtggt ggccacggtc agcgcactca cccccgcccg cacgcggtaa ggcagttcca
    26641 accgttccaa ctcgtcagtc tcttccgcca gccgcccgaa caatccgggg tcacccccct
    26701 tcagccgcac gacccgtctg ccctgacggg catacttcgc gatgaggtca gtaatatcgg
    26761 gctgccgcac cgcatgttcg ccgcaccgtt tacccacgaa gacgcgctcg gccgagggcg
    26821 gcagaaactc cagcaaccgg tcatccatca gcacgtcgta gaggcatacc tccgcctgcc
    26881 gcaaatcccg gacgccgccc caagtgcagg actcggccac gcccaccccc gcgcccacaa
    26941 agcgcaccgc tttgacaaaa tatccgcgca ccctcgtcca gaccggatcg cccagtcgga
    27001 aggtcaccgc caaccgtccc tgaccggcgg gcaccggcag ttctgaaagc ggtatccact
    27061 cgctcacgcg ctcttccaga ccgagccggt tcagagccgc gccagccatc agcagagcgt
    27121 cataccggcc agcatcaagt tgctccaggc gcgacgggat cgcgccgcgg atcggcttca
    27181 gcacggcgtg cgggaatcgc gcccgcgcaa aggctccgcg ccgctcgcta ctgaccccca
    27241 ccaccggctt ctccggcagg tcagcccacg ttttaccttg cggcagaacc catgcgtcgc
    27301 gcggatcttc cccccaaggc agccagaacc agtccaaatc cggcggcacg ggatcaggca
    27361 ggtctttggc gctgtgtacg gccaaatcga tgcatccctt gcgcaaggcg tcatccaaat
    27421 cgcgcgtgaa aaaatccgcc ggactgacgc gcaggtcggt ggccagatcc cggtcacccg
    27481 gcgtttccag cgtcacggga tcgaattcga gaaacggaaa cagcgcggca atccggttga
    27541 gcgcgtcacg cgtctgagcc agcgccagcc gactgccccg cgtcccgact ttcgcgcgta
    27601 ccgtttgcat ggttaacctt tccttcactt ccgaacgagc cgtaccgcaa catgcccatg
    27661 agtaaacttc atgctcttcg tatccttgtg gtgtaacaac atgttattgc gtttgttgat
    27721 ccgtaaggca ctgccgaaaa cttcgacgtt cagcgttcga cgttcagcgt tcgacgttct
    27781 tccccggtct catgttcccc ctggacgctg cgccgcatgc gctcatagag gctgcggtgc
    27841 tcgcgcagga tcgcgtcaca tgctttcagc actttgtcca atgtgccgtt ggcggaccgg
    27901 taccaccgtt tgatctcatc cagatccgcc acgcgtaccc cgttgccgaa cgtgtccagc
    27961 gccggatcga tattccgggg catgccgagg tcgatcagcg tcacaccgcg cggattcaac
    28021 cgggcaccat gctccaaccg gttcagcacc ggctcggatg cattgaccgc actcaccaca
    28081 gcgtcaatcg tgggcagcac acgcgccagg tctttcagct ggatcacttc gatccggtgc
    28141 gcgtcttggg cgctcgacgc gggcgcgttg cgatgatagg cccacacgca atggcagccg
    28201 cggcgcagca gatcctccat cagcccctgc ccgaccaccc ccgtgccgat caccagcaca
    28261 cggctgtccg gcagcgccgg cagctgttcc gccaagtacc gcaacgccaa atcctcgatc
    28321 tccgcgacgt ccagcagcgg ctccacctca tgacggatat ccttggaaat gtgcagcgcc
    28381 gcatcgcaca actcccggat gaccgatccc gcccaaccga acgtgacggc ctcatccacc
    28441 gcctccttca cctgagagac cacatggaac tcgcccggcg tctgcgaatc catgccagcc
    28501 gcgaccaggc acaggtgcgc gaacgcgtca aacccgcgct tcatgtaaaa ccggtccggc
    28561 ggcaattggt cgaaccgcag caacctccgc aaaatcccgc tggttcccgc ctcgcgcgaa
    28621 atcgcggcca ccacttcaat acggttgcag gtgttgagaa tgaaaaactc atgcacaccc
    28681 cagatctgcc ggatcatccc gccaatcgcc tcgcgttccc gcaacagcaa atggaatggc
    28741 gcacgttcct cagccgaaag gtactcatga ctggtaccca gcaccagcag atccgaattt
    28801 tcgatcgaat ccagatgttt ccgaagattt tctttcacca gccgcgattg ccgacaggat
    28861 ttgccgctgg tggagacggc gaccagcaca tccccggtgc ggataatagc gggcgtcaca
    28921 aaatccccgt ccggccaatt cccgtccgcg cagcagcaca gaatccgatt ggcttgtgcg
    28981 ctttcgagaa cggttcggtt gacgcgcttg tcatccgtgc aggcgaagac catcgccgcg
    29041 ccctccacat cggccggctc aaaccggcga gccgtatggt ggatcgcccc ggtttcggcc
    29101 agggtttgca attccgccac acagtccgga cacacgagcc gaacctcggc gcccgaatcc
    29161 aacaaaagct cgactttgcg ctgcccgacg cgtccgcccc ccacaaccaa cacgcggcga
    29221 cgctcaatca tcgcgttgat cggaaatgtc tttcgaaccg gtcttgtgtc tcgcatagat
    29281 cagaaaaacc gtatcacatt cctgagcgct tgaccacaca cgagaccggg aagacccctt
    29341 tagtcgatcg cgtcacccag ctccatgttg tcgagatcga tctccccgct gaacacaaac
    29401 ttgaccggac cagacagcgt caatccggta catttgcgat gacgccagtc gccatccacc
    29461 gtgaggtcgt atcccgagga tgtcttcact ttggcgggca gcgtgaaccc tgcggtctcg
    29521 accgccacca ccacgcaggc gacggccccg gttccgcagg caccggattc cgcttcgacc
    29581 ccccgctcgt aggtgcgcat cgtcatccgg ttgggtgcgc gcaacgtgac aaaatccaca
    29641 ttcgttccgt ccggcgcgaa aacgggatgg cgacgcagcg cgcgcccgag accctgcaca
    29701 tccaccgcgc tgatatgagg cacctgcacc acaaaatgcg gcacgcccgt attcagaaaa
    29761 tccccgcgaa tcgtctcccc gtcaatctgg acctcgatcc cgtagttttt gcgggtcggc
    29821 tccggcatcc acacgcaaac cccgtttttg gtgagctgcg cgtccaccag gccgcacatc
    29881 gtctccatgg tgagggctcg gccgctggca ccgatggcat gcgcgaacgc cgccgcgcac
    29941 cgggaaccgt tgccgcacat ctccacctcg gtgccgtccg ggttgaggaa ccgcatccgg
    30001 aagtcggctt tatccgaacg ctgaagcaga atcacccctt cacaaccgat tccggtgtgc
    30061 cgtcccgcca aggcggccat cagaaaatga tcctcccacg gaaccgttcc ctcccggtca
    30121 tcgatcagca caaaatcgtt tcccgcgccg tgcattttcg tgaaacggat tttcattcag
    30181 tccctcctcc ggcttgtccg cgttacggca actcccgtcc gaaaatgcgc atcatgcgcg
    30241 tccgtaaatc gccctccgtc tccatcggga tctcgccgcc gcccgacaac gcgaccagat
    30301 cggcgaggat atgcatcgcc tcatccagca ccacatcgtc ttcccattcc tcctgctccg
    30361 aagcatccga cgcctcatcc tcaattttcc gcatctcgcg ttcagcgcgc atcatcgcgc
    30421 ggcgcgcctc ccactccaac ggcacctcgg tcttctcgtt caattcctgc acatgccgga
    30481 ccagagtgca ataccgtgcg taccggctgt tgtccgccag gcgttgggct gagaaagcct
    30541 tcaactgcgg cacaaacgcc gccacatccg aaaccggaat gtagagtgcg gattcaaccc
    30601 ggctccaggg cagggcaccc ggcatcttgt cttccccgat gtccagcccg tcgagcgtcg
    30661 acggaatgac gatatccgcc gccacacccc ggcgttgcgt ggatgcgccg ttgatgcggt
    30721 aaaaactcgc ggtggtcacc ttcatggatc ccattttctc cgaacctaaa ggcatcaccg
    30781 tctgcacggt gcctttgccg tgcgattgcg tatcaccaac caacaccgcg cggccgtaat
    30841 cctgcagcgc gcccgccaca atttcggaag ccgacgcgct ggcgcggttg atcagcacca
    30901 ccaccggctt gcggaacgcc atggccggat cgagattggg gacagggagc accaccaact
    30961 gacgggcctc gcgcacctga accgcgggac cgctccgcac aaacaggccg gtcaggctga
    31021 tcgcctcgcg cagcgacccg cccccgttgt tccgcaaatc cagaatcagc cccgcgacct
    31081 gttcgctgtt gaacgcggcg agttggcggg cgacgtccag cgtcgcgctg cggaaccccg
    31141 cctgacccgg gcgtttgtcc attgtaccgt aaaacgtggg cagccgaacc accccgacct
    31201 tgacggtctt gccctcgggg gaggtcaccc gcgcgacacg gccggtcgcc gcctgctcct
    31261 cgagtttcac ctcatcccgg atcaggtcaa tgcgcttggt gatcgtcccg ctaggctccg
    31321 aagcgggtat gacctccaac accacccggg tccccttctc gccgcgaatc ttacgtaccg
    31381 ctttgttaag cggcagatgc acgatatcct cgatcggtcc gttgccctgc cccacaccga
    31441 tgatcttatc ccccttcgtc aggcgctggg cgcgcttgtc ccggtcggcg ggcccgccgg
    31501 ggatgatctc caccaccaac gcggcgccgt cttcggaccg gagttgcgcg ccgatcccgc
    31561 agagcgagag attcatgtca atgtcaaaat cctctttacg catgggcgac atgtaatccg
    31621 agtgcgggtc atacgccatg gccacggcac tgaggtaacg ctgcaacacc gcctcctcgt
    31681 ccatatcctg cacgacaatc atcaactgct tgtagcgctt ggcgatgttc tgctcgggcg
    31741 tatccgttac cgtactctcc gcacccggca tcgcgttcgt aacggcctgc accggggcat
    31801 tggtcacacc gagccggtcc agttcgcggc tgatgcgctg cgacagcacc tcgtttttga
    31861 tgcgtttgcg ccacaaatca tcctgctcgg tcaaagtcgc gggccaaggc gcctcccgcc
    31921 gtttccaggt gtagctttcc tcctgatcaa aggtgaaccc ttgctcaagc aggttggtga
    31981 cggcctgata gcgctccgtg acccgcttgc ggaaaacccg gtacacctca aaggggaacg
    32041 agacatcccc cgccttcaac gcatcatcga tctgggtccg catcggaatg aaggcgtcga
    32101 tatcgctctg cagaaagtag ctgtggtcga aatcgaacgc gttaagcagg ttggtccagg
    32161 cgacctgaga aatggcgtca ttgaggggtt tctgcgaaag atgggcagct ggcagcaccc
    32221 gcgcgacttt ctgcgcgacg ccgccataat ggggctgcgg caccagcgta gtctgtgccg
    32281 cgcccgctat ccgcggcatc atcccgagca cgaagagcgc caacccgaaa aaccggcatg
    32341 tcctcatcat acgtctttct gctccaatac cttcacggcc cgttcgatct catctgcgga
    32401 cagatgcccg ccccgttccc gcatcacccc cgcaaggccg ctcacattgc agaccacgca
    32461 cccttcacac accgaaaccc ccgggcggaa ggtgttgctg gcgaaacaga caaccggcac
    32521 acaggccggt gacccgccca cccgctcggc caaaaaagat gtcactgccg ctgccgaggc
    32581 ccgtgcctgc tccaacggtg gcctgctcgg caaaccgcca tccacccgca acacgccctc
    32641 ctccagcgtg acggaaccag accagcactt cgtttcgatc gcaaacaggc cggtcggccc
    32701 caccaccaca tggtcgatcc cctccgaacg cccgccgcaa agaagatcat gaaacacatg
    32761 aaaaccgcgc ggcagaccgg aaagcaccca ggccacccgc tcttccccac gggccccttt
    32821 gaaaaaagcg tccagtccgg caagcccgtc acgcaccgcc cataccagcg ccgccgcggt
    32881 cacaagcagg ccgagccccg ccgcctccca cgtgatccgc ggcagcagca gccccgaaaa
    32941 cagaccgaga acgaacaggg ccgccagcag cgggccgacc gcccgcatca ccccggccgc
    33001 acgggcgcgt tcgcccggaa caccgtatat ccgcgctgac tccggctctt gcataatggg
    33061 ttcaattatt gtgaccgatt cacacaccgg ttgcaaccct cttcttgccg gcgatctgcc
    33121 gggccatgtt ttcggcggtc agtccgcatc cctccagcaa ctcggcgagg ttcccttgtc
    33181 ccacaaaccg gtcgggccag ccaaaggccg acacaggaca atcaaaaccg ccagccgcca
    33241 gagcctcgcg caaagccgag ccgaacccgc cgctgaccgc gccgttctcc attgtcacga
    33301 attgcgccgc gtttccggcc tgagcataga gcagcgtcgt atccatcggt ttcacgaacc
    33361 gggcattcac cacgccggaa cgcaggcccg cgtcccgcag acgggtcgcc acgtcgcgcg
    33421 ccaacggcag catgtcgccc agtgcccaaa tccacacctc gcccccctcc ggccccgcat
    33481 ccggatgaag caagacttcc gcctgtccga cggacaatgc cgggaccgcc gcgtcacacg
    33541 ccacctccgg acccggttca cgcggatacc ggatcaccga cggcccgtcg agcgcgaggg
    33601 ctgtccgcag catggcctcc agttccaccc gatccttggg ctgcatgatc gtcagattcg
    33661 gcagacagcg caacatgggg atatcgtaga ccccgtgatg cgtgggtccg tcgcccccca
    33721 ccacgccggc ccggtccacg cagaacacca ccggcaggcg cgggatgcaa acgtcctgca
    33781 tcacacagtc gaccgcccgc tgcaagaacg tggaatatac cgcaaacacc gggcgcaagc
    33841 cggcggtggc caaacctgcg gcgaacacca ccgcgtgcgc ctcacagatt cccacatcga
    33901 agaatcgttc cggatggcgc gacgcgaacg gctccaaccc cgtgccgcta cacatggcgg
    33961 cggtgatcgc cacaaccgac gggtcccgtt ccgccagagc ggtcagcgtt tcgccgaacg
    34021 cctgcgaata tccgcccgga cgcgcctcag gcggttggcc ggcctccaga tcaaacgcac
    34081 ccacgccatg ccaggcgctg ggacggcgtt ccgcgggccg aaacccccgc cccttttgtg
    34141 tggcgacatg gatcagtacg gaacgcttgt attcgcgcgc cgaacagagg gcattctcca
    34201 acgcgcggaa atcgtgtccg tcgatcggcc ccacgtaccg caggccaaag gcctcgaaga
    34261 aagcgttctg cagccacagg ctcttgatcg cctgttccag ccggtagtaa atccgacgca
    34321 gcggtgtcat gcgcaaccgg tggccggccg cttcggcagc ggccttgatg cggttgtagc
    34381 gcacatccgc gagcatccga ccgagacggc gggagagcgc gcccacattc tcggagatcg
    34441 acatctcatt gtcattgagg atgacgatca ccttgcttcg cacctcctcg atgctgttga
    34501 gcgcctcgag cgcgatccca ttggccatgg cgccgtcgcc gatcaccgcg accacatgcc
    34561 ccgcgccgtt cccccgctca cgccctgccg ccaatcccag agcggatgaa agcgcggtcc
    34621 cggcatgacc ggccacgcat acgtcacagt cactctcttc cggccgggtg aaccccgaaa
    34681 gcccgtcccg ctgccgcagg gtgtgaaaac gctcccgccg ccccgtaagc agtttccagg
    34741 cgtaggtttg gtggccgaca tcccaaatca ccttgtcctg cggcggagaa aaggcgcgca
    34801 gaatcgcgat ggtcagttcg accgcaccga gattggaggc caaatgaccg ccgttccggc
    34861 ttaccgtctc cagaatcgcc tcccggatct cttcggccag gaccggcaac tgtccagatt
    34921 cgagaacctg cacatcagcg ggagtccgga tcgttttcaa caaattcgac ataacctcac
    34981 tctgcggctt tctccgcggc cgaggtccga cgcttccaga tctgaagcgt cgtgccgttc
    35041 tgcccgaagc cgggaacctc ctgcacacga tcgtagtatt tgccgacctt actgaaaaac
    35101 atctgccgct cggcgtgcgg cacctcatcc atcaccggcg cacgcacggc taacccatat
    35161 ccgctgaagg ccgccacccg gcacgacgcg gacgtgatca gttcgctcaa acgccgggta
    35221 ttcatcacat gaaaccgctg cgcgtcctcg tctcccagtt ccggaaaata actgaacggc
    35281 cccatttcca gcccatccgg cacacgcatc ccggcctcca ccgcaagata ggtgtcctgt
    35341 gtcagcaaca gccggtcctc ctcagccagc gcccgcacgt cacgggccgt catccggagc
    35401 agcgccagat caggcatcgg cttacgaacc acccagaacc ggtcctgccc gtgcacgaac
    35461 cactcctgcg ccagcggtga ggcaaaggcc gcgagcagcg aggccacggt ccagaacagg
    35521 caaagacaac cgcgcaacgt tccggaggag gtgctgttcg cgacccaccc cgccagcgca
    35581 gcagcgagca accccatcac cggaacctga tagtcgtcat acgggaaggg gctgagcaga
    35641 tgcaccagaa agaccgcgag aaaaccgagt gtccagattg cgggccacat ccccccctga
    35701 taccgaacgc ccgccggttc ttcttgcgcg ttctgtccca ctatgccgct gcggaagatc
    35761 cggaagatga tcagcgccgc agtcaacagc gccatcggca aataggcgcg gactgtccgg
    35821 gagaatgacc cggccagata gaaccaatcc cggccgccgc gcgagacatg atacgtctgg
    35881 ccaaagataa acgcttcgcg cgcctccatc agacagggtc cgtacaccag cgccagcccc
    35941 actcccccac ccaatgcgaa ccagaaaaac gcggctccgg tcttacggca ggtgaccagc
    36001 aacgccagcg cggtaaccgg cagcagcgcg cccagtgaaa gccgtgtgcc cgccgcaaac
    36061 gcgatcagga gaccggcagg cagcgcccac atgcaggaga gggcggaacg ggtcttacag
    36121 cgacgtgaca cgcacagcga cagaaccccg tatccgccca gaagcagaca ggaggccagc
    36181 gcataggttt tggggatcgt agtgaaatag atgtgataca gattgcatcc cgtcaacgcg
    36241 aacgcgatca ggccggcttc cgccgcccgc tgggccggca ccgcgcgccg cgccaacccg
    36301 gccgccatga cacaccccgc caaccccatc agtgccgtca gaatccttcc gcccaaaacc
    36361 ccaaaaggtg tccagaggcc cgagaagagc gcatacacgt acggcagaag cggcccctgc
    36421 gtgaaaaaga aatcccggta cggtctggcc ccctcggcca cccggatcgc cgcatacagg
    36481 taccacccct catcctgatt gaggttgccg aaccatacgg cgaaagcgta caacacaacc
    36541 gtagcccccg ccaacagagt cgcggcacac cctaaacaaa agcgatgact cccttttcgg
    36601 agcatgtccc tccccccaaa aattacccgc cgcgatgccg catcctgtcc gctctcattg
    36661 cgcccttagt ataacccgat ttctttccgt cacgcaagca gacagccgga tcaccgcttg
    36721 acccgaggcc ccgcttccgc atactatccc cgcatccgga aaagacacca aaggcggaaa
    36781 agcaggcgcc gccaccagcc cggtgaaccg ccgcggggcg tgaaccccgc acgcatgtac
    36841 tcgcgatctg aaagtcccat gaatgcgttt cctgactctg tcactccacc gcccgcagcc
    36901 cttccccgcg acacgccggg cgaacggctg cagaacgtcc tcgcgcaccg cggcgtcgca
    36961 tcgcgccgcc atgccgccga catcatcgca aacggctcgg tcaccgtcaa cggcaaggtg
    37021 atcttcgcgc ccggattccg cgtcgatccg acccgcgatg acatccgcgt caacggggag
    37081 cgtcttgcga agacagagga acgttcccgc acgatcctgc tccataaacc cgcaggcctg
    37141 atttgcagcg cggactcttc gcgtggccaa acggtctgcg acctgatgcg cccgcatttc
    37201 cgcgaacgga tcgtgcccgt ggggcggctc gacaaagaga gtgaagggct gctgctgctt
    37261 tccaatgacg gcgaactcat ccaccgtatg acgcatccgc gatacggtca ctccaaaacc
    37321 tacctcgtcc gggtggcggg ccgcatggcc gcttccaaaa tcgaactgct gcgctcgcgt
    37381 ttggagatcg acgggtacac catccggccg gtgaaggtcg aggtgctgaa ggagggacgc
    37441 gacaacacgc atcttctggc cttcaccctg agcgaaggcc gaaaccgcca gatccgcaaa
    37501 atgtgctctc tggcgcattt cgtcgtgctg aacctcaccc gcgtacgcat cggcccgctg
    37561 aagatcggca cgatgcgccc cggcgaatgg cgggaactct cggaagccga agtccgggca
    37621 cttaagcgca cgccttccgc atccgtcatc ccgccgccgg atcgcggcgg cccgacccgg
    37681 ggcggcacgc cgccccacgc caggccgaaa ccggacctca ggccccctgg cccgcgaaaa
    37741 agcggtccat cagcgcgctg tgctcggcat ccgtgaaccc gcggctctcg atcgtgaccc
    37801 agccgttgta gccgatctca tcgatcacct tgcgcacgga gcgccagtcc acgctgcctt
    37861 ttccgatcgg cacgaaatcc ccctcgttct tcttttcgcg gtcgattttg aaatccttga
    37921 tgtgaagttt cacgatctgg gaacccaacg cgcgcagcca ctcctcggtc ggggcgtacc
    37981 gcacgttgtt gccgaggtcg aagtaggctt tcacccacgg gttgtcaaac gaccggacaa
    38041 acgcggccgc gaaatccggc agcacccaga gattgttcca cacattctcc agcgcgatga
    38101 tcaccccttc gcgcgccgcg acaggaatca gttcctcaat cgcgcgccgc gacatctcgg
    38161 tcgcctgatt ctgcgcggcg atgtactcgg caaacggcgc gttgtccccc tcgaccaccg
    38221 acgtgacgcg cagcgtccgg ggatcaaacg caatccggaa ctgcgacggc ttgggcatgg
    38281 tgccgcccac cctgcacggc accagcagga taacggagga tccatacgtt gccgtgaggc
    38341 ggatcatcct cttgacgtcc tcgatcgctt tgcgccgctg ggcctcgtcc gagctgttga
    38401 aattcgtcca cccgcccatg aaagaatgga tggcgagtcc gtgtttcgcg gccgtcaaac
    38461 gtccttggat cgcctgttcc tcggtcacgt tcttattgga cagttcgaca ccgggaaagc
    38521 cggccttggc gatccgctca caggtcttgt catccgccac acccgcaatt aacgccttgt
    38581 acagcgtggt ccggaacgcg catgcgcccc ccccctcctg cgcgaccccg aaacgcggca
    38641 tccccgtcac tgccccggct gccgccacac ccatcatgaa tgccctgcgg ttcatctgca
    38701 tgtccatcct ccccttgcct ttcctgtgct cccgttccgt tgggctgcac acccttttcc
    38761 ggatttccag tcccatcgca tcaggcgcat gcgcctgttc ccaatttgac gtaaacagta
    38821 tagagacccc ggaacgtcta tgtcaagaca cacgcggtca ggacagatcc gcttagaatg
    38881 gcccgctacg cgagtgcccc cgggtcagca gggggtttcg gagttgactg cgttcgcctc
    38941 ccgcctgcat aatgcgtttc aaccacaaga ccggcacggc acctgtaaag gctggggaat
    39001 gccggcggct cgaataatct cttgccgagc atgacccaac tgatctgttg gcaatgccca
    39061 aaatgggggt aagcgggatg aatcgaaatg cgggaacgtt gttggcgatc gtgctttcat
    39121 tgcaagtcag ggcagcgggt gatccggcag cggtagcggc gtgggaaaag tcgggacgcg
    39181 cggaaacact cgaatggttc aaacgccatt ggttcggcgt gcgcccggcg gaacgtttgg
    39241 cggatcaaga gatcggcgag gattacgtcg cgtgccacgg cgggcagatc aggatcaaca
    39301 tttacgtgaa gcttccggaa ggcgcgagtg ccgagaaccc ggtgccggcg ttcatcttcg
    39361 gcgaccacta caatcccaag acaagcagaa ccaagacaga cacgatgccg ggaatcccga
    39421 cgaacgcgat cgtctcgcgc ggctacgcct acatccggtt caatttcaat gacgtgacgc
    39481 ccaacaccaa ccaagggtgc cacgacccgt caagcgccaa catgaaactc ggcggaatgg
    39541 gcaatcccga cagctggggc acgatctcgg cttgggcgtg gggtttctct cgcgtaatgg
    39601 actgggtgga gacacggccg gaactggatg cgaaacgcat cgcggtaatc ggacactccc
    39661 gcggcggcaa gaccgcgcta tgggccggat gcactgacca acgcatcgcg ctcacgattt
    39721 cgaactgttc cggtaccggc ggggcgcgtt atttgcatat gccgctcaaa ggaatggaaa
    39781 cgctggactg gatgaaagac catgcgacgc ggcactggtt ctgtccgagc tggataccga
    39841 cctatcgcaa ccgcgagacg gaaatcgagc atgatgcgga cgaaatgatc atgctcatcg
    39901 cgccgcggcg agtctacgtg gcaagcgggt cggaagatag ctgggcaggt cccgcaggcg
    39961 agttcgaatc ggccaaacgc gcgtcggcgc tctgggaagc ctatggacac aaggggcttt
    40021 cgctcgacac gttcccggtg ccgggtacgg tcgaccactc cggccgggtc ggataccatt
    40081 tgcgtccggg agcgcacgcg atcaggccgt gggactggga ccgctacatg gacttcatgg
    40141 acaaacatct gccggggccg aaaaaggcac ccccgaaagc gatggagccc gcggcaccgg
    40201 aatcgcaacg tgtccaatca gaggccatcc tcgcgtggat caacgccttg gaagcctttt
    40261 tcgacggcgg cgagcaaggc tctgttcacg gcttttcgat cctccggcac ggcaaagtga
    40321 ttgccgaggg cagttgggaa ccgttcgaca cgctgcatga gacacacatg ctctattcgc
    40381 tctcgaaaag ctttgcctcg accgcgatcg gattgctggt ggatgacggc aagctggact
    40441 tggacgaacg gatcactgac ctctttccgg aatacgttcc ggcggaacca tccgaaaacc
    40501 tgaaggcgat gcgtgtgcgc gatctgctct ccatgaatgt cggcacgagc catgccgatc
    40561 cgcacaagaa agaccccggc ggcgattggg tgaagatgtt cgtcaccaac gtgatcgacg
    40621 gcaagcccgg ccaggctttc aggtacgaca gcagcgccac ccatgtgctg gtcgcgatcg
    40681 ccgagaagag aagcggcagg acgtttttgg atttcatcgg ggagcggctc ttcgaccgga
    40741 tcggcatcgg gaagaatgcc tggaccactt attcgccgac cggtatcgct tgcggcggat
    40801 gggggctcaa tctgacaacc cgcgacctgg cgcggtttgg acaactttat ctgcagcgcg
    40861 gtgtctggaa cggcgaaagg gtcttgtcgc gagagtgggt agaactcgcg acagcccggc
    40921 acacctggaa cggcgggatt gtcggcacgc gtccggatga cgactgggcg caggggtacg
    40981 gcttccagtt ctggcgctgt gttcccgagg gcgtttaccg tgcggacggg gcttatggcc
    41041 aatacacgat tgtgttgccc gatcaagata tggtggtgtc aatgcacgcc ggggtcaaaa
    41101 gcatgggcaa aatattaaag ctgacctggg aacacctttt gcccggagtc aggaacgtcc
    41161 cgttgccggt gaacacggtc ggtgaggaag cgttgcagaa acgtcttggc ggtctcgcca
    41221 ttgccccggt aaaaggctcg gtcgcggaag gcgcgtattt cggccgggaa ctcgctctca
    41281 aggaaaacaa tcgcggcatc aggtcaatcc gcctcgaccg cgaaggcgaa ggatggaagt
    41341 gtacgctcgt gacccgcgcc ggggaaaacg cgttcccggt cggatgcggc gtttgggcaa
    41401 aaggcacgat caggatcgat acggagaaat acgaaaagcc tggcggactt atcggagtgc
    41461 agccgacggt ggcttcgggt ggcgtggatg cggacggcgt cttccgcata aaggttttcc
    41521 tgaccggaac aaccgcttac ctttcgttcg aaatctcaga aaagcagatt ctgggccgac
    41581 tttttgccat agacggatgc cggtttgagg ggaagacgga ataaattggt ttcagggttt
    41641 cagggtttct ggtttcaagg tttcagggtt tcagggtttc agggtttcat ccaaatcggg
    41701 gtcgggatcg ctatcgggat cgctatcggg tttcaaggtt tctggtttct ggtttctggg
    41761 tttctggttt cagggtttca aggtttcatc gctatcggga tcgctatcgg gtttcaaggt
    41821 ttcatccaaa tcggggtcgg gatcgctatc gggtttcaag gtttcatggt ttcaaggttt
    41881 ctgggtttca tccaaatcgg ggtcgggatc gctatcggga tcgatcattt caccacggaa
    41941 caccaccgcc tcacagcctc acagcctcac agcctcaccg cctcacagcc tcaccgcctc
    42001 acagtctcac cgcctcacag cctcacagtc tcacagtctc acagtctcac agcctaacag
    42061 cctcacagcc tcacagcctc acagcctttg agat
//
LOCUS       DAOSRB010000003        38053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1204_length_38053_cov_6.303200, whole genome shotgun sequence.
ACCESSION   DAOSRB010000003 DAOSRB010000000
VERSION     DAOSRB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 38053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38053
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_38053_cov_6.303200"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..766
                     /gene="trxA"
                     /locus_tag="PLZ74_00340"
     CDS             335..766
                     /gene="trxA"
                     /locus_tag="PLZ74_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOR96802.1"
                     /translation="MRMRIFATVSVIGCLWLTGCKPAGAPTTDSVPDAGSGTAAQVLE
                     LNDMTFTAQTAQGVVLVDFWAPWCGPCRTQGPIIEQAAKLLGDSAKVAKVNVDDAQQT
                     AKRFGVQGIPTLIVFKDGKPGKRFVGVTQAAVLVEAVKEAL"
     gene            895..3636
                     /gene="polA"
                     /locus_tag="PLZ74_00345"
     CDS             895..3636
                     /gene="polA"
                     /locus_tag="PLZ74_00345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOR96803.1"
                     /translation="MSETAKLFLIDVMPLLYRGHFVFLKSPRVTSGGVNTSALTGFAN
                     CLLQILNEHTPTHVALVLDSSTPTFRHEAYPLYKAQRQKMPEDLAAAIPMSIELAEAL
                     RIPILRVDGFEADDVMGTLAAHAATKPGWQTYLATPDKDVAQLVGPATFLFRPGKGGA
                     PAEIYDEAAVCRHWGVSAPSQMIDLLGLAGDASDNIPGIPGVGEKTATALLAQFGDLE
                     NVLAHAAELKGKLAEKVAAGAASARQSRELAAIRTDVPLTIRLDDLACRDPDEARLRA
                     VLKKYELAALGKRLLGEGFTDGTGGVAASGAYQTLADVPHTYVCVTDEERMRELLRAL
                     EEAPEWAFDTETTGLNPRHDRLVGLSFATAPGRAWYVPVPETHEACRAALHRFAPYFD
                     DPATIKIGHNAKFDLTVLRQYGITLRGELHDTMLAHYVIDASDRHGMDHLARQYLHYN
                     PIPITALIGEKKRGTVQGNMGDRAPEEICDYAAEDADITLRLDRLLRQEAQETGCLRA
                     LTECEEPLVGVLLDMESEGIRINEQALREYGRELDREILDLEIRIREFGGGPFNPASP
                     KQLGEVLFDHLKLDPDAARTASGQYATSEEVLLKLRDRHPIVPLILEYRMCGKLKSTY
                     VDKLPDCIDPETGRIHTSFSQALTETGRLSSSDPNLQNIPIRTERGQRIRSAFVARDA
                     DHVLLSADYSQIELRVMAALSKDPGMIEAFRRGADIHRETASRVYGVPPDEVTPEMRS
                     VCKMVNFGIIYGISAFGLGQRLDVPRKQAAELIETYFTTYPGVKTYMDQAIAEARAKG
                     YAQTLLGRRRFLRDINSRNATSRQSAERNAINTPVQGTAADLIKLAMVRVHRELAERG
                     LKTRMVLQIHDELLFDAPRGEVETVRELVTQAMTTVWEIGVPLEVSVGTGETWLEAH"
     gene            3691..4284
                     /locus_tag="PLZ74_00350"
     CDS             3691..4284
                     /locus_tag="PLZ74_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOR96804.1"
                     /translation="MNEKEGQEVTRRNALAWLLRVGAGAAITAAAVQATRRASGKRTV
                     WQIDPRKCIQCGRCETECVLHPSAVKCVHGYAMCGYCKLCFGYFQPDAARLDEAAENQ
                     ICPTGAIRRQFIESPYFEYHIDEARCTGCALCVKGCTHFGNGSLYLQIRHDRCVGCNA
                     CRIAEACPAQAIMRVSADTPYLLKEKLGPSGPKESDG"
     gene            4281..5591
                     /locus_tag="PLZ74_00355"
     CDS             4281..5591
                     /locus_tag="PLZ74_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR96805.1"
                     /translation="MKRPGCIRKRAGLAGVLLPLVTWAADPRFPQPQFESGHTLPQTP
                     HPAFVEQVPAWMDVLLLALALGIAVWLVRRLRSRRGVLLFSLLCLGWFGFVRRGCVCP
                     VGAVQNVAEAMWNGGGLPWFVAAIFVLPLFATLLYGRVFCGAVCPLGALQDLFIIRPQ
                     RVPRAMDAVLRLVPLGVLALGVVAAVNGAGYLICQWDPFVGLFRRSAPMTVMLTGMAV
                     LFVGMVVARPYCRYFCPYGVLLEFCAGLAWRPVTIPSRTCVNCRLCVGTCPVGAVTAP
                     REVTSGAERKRLFRRFVTLAVLMPLGVLLCASTGWLTGAWVARAHPAVRMAEVLATVP
                     QEAADQHPELEAFRRGGASRDEAEAYAAEAVRRFRAGGAGAGVFVGVVMGLRLLGMTR
                     LRRRPLHEADRMRCVACGRCWDACPLNRSVAGTSGSTSDGPSVG"
     gene            complement(5580..6431)
                     /locus_tag="PLZ74_00360"
     CDS             complement(5580..6431)
                     /locus_tag="PLZ74_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR96806.1"
                     /translation="MANSDRDMPLLRIRGLKTWYPVKKGVFARTVGHIRAVDGVDLDL
                     MRGETLGIVGESGCGKSTLARTILRLEKPRAGTLLLNGRDIRTLRGADLRTYRRTVQV
                     VFQDPYASLNPRHTVLDLLTEAMLAHRLVTPETRRDAAVRLLADVGLPEEILDRYPHE
                     FSGGQRQRLCIARALALGPQLLICDEAVSALDLSVRAQVLNLLEDLKAKHGLSYLFIT
                     HDISVVQHIADRIAVMNRGRIVETGPAEQVLNTPEHPYTRLLLSAVPRIGVPLPDFPE
                     PALTSTH"
     gene            6614..7858
                     /locus_tag="PLZ74_00365"
     CDS             6614..7858
                     /locus_tag="PLZ74_00365"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOR96807.1"
                     /translation="MNKKTLRDVDLKGKRVVMRVDFNVPIKEGVIKDDTRIRGALASI
                     RYVLEQGGSLVLMSHLGRPKGKGYEEEFSLKPVAEHLAGLLGKPVTFAPDCANADAEV
                     SALQPGGVVMLENTRFYKAEDGKVKKTDEMSDEEVARKKAELKAKREELAKKLASYGD
                     LFCNDAFGTAHRAHASTAVICKFIPESVAGFLMEKEIEYLGNAVEKPTRPFVAILGGA
                     KVSDKLAVVKNLLDKVDTLIIGGGMAYTFLKAQGHDVGGSLCELDQLAYANQMIEKAK
                     ANGKRLLLPVDNVVADKFDAEANTQVVGNDIPSGWMALDIGPQTTELYATAIKGAKTV
                     VWNGPMGCFEMAKFAGGTTGVCKAVAESGAVSIIGGGDSVSAVNRSGLADKMTHISTG
                     GGASLEFLEGKELPGVAALSDK"
     gene            complement(8008..10425)
                     /locus_tag="PLZ74_00370"
     CDS             complement(8008..10425)
                     /locus_tag="PLZ74_00370"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOR96808.1"
                     /translation="MTTSRPTLLRCLLLVAAGLVSAADLTLQNRAVLLQYREASGEVT
                     LWVPGESKPFARFDAATQKPSAVRTVRLNEPRLGAGEALEFDFPHGSRNRVILFADLP
                     FAVLRTSWRGTNTTNPVNRIAYPAVRLALDRPAEELTSLGTGGLLQPKRNPGSYMWTA
                     VADPATRRGVVAGWLTTDRGSGIVRMAVAEECVTLLPHLDYGRLAPNADREEDLELFA
                     LGLFDDARFGLEAYADAIARIYAIRLPPMPTVHCTWYVDRASNERVMTDRTAYAAQTL
                     KPFGLRVMQIDDGWQSGQSTNGPKKVFIRHRPDGPYPAGMKAMSDTIRAAGMTAGIWL
                     IPFAGSREDPWFADKTAWFAKRSDGSPFDTRWGGTCFDLTRRDTQAYLRDMIRTLTAE
                     WGYRYLKMDALYAGAAINLNYVCNTFREDEIGQAILTDPSVTPIQMMRNSLKIVRDTA
                     GRDVFLLGCCTPQNMRSAGAAFGLVDAMRIGPDNGANWSAMMRGPEYGAWNYFLHGRV
                     WYNDPDPLYVRPSLPIHQAQALAAWVTLSGQMNSSSEHYAGLPPDRLDLLRRTMPSHQ
                     AVARPADLFENRIPGIWLVTDGATAERPQRQVAGLFNWDGTNKTFNVPLASLGLETRS
                     PWIGFEYWGNTLTPPFRHSLPLTLPPQSSAVLALCPIRDRPQLISTSRHITQGITDVR
                     AVTWDPVSRTLSGVSDTVADDRYELRILTHADASASFGTVLSAAITGPNGYELTIAPG
                     EGQVTRPDDAYAHANTPDAPRTTLFEPKSEEGLVRLAFISGSGGQVSWRVIFSERTIS
                     ALTPPAP"
     gene            10719..12065
                     /locus_tag="PLZ74_00375"
     CDS             10719..12065
                     /locus_tag="PLZ74_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOR96809.1"
                     /translation="MRLTGRGLGTMWLAAVCAAAGIVSAAPEYRQVAAEEVRPRGGIG
                     NVMRKIRAGGEVTVAYLGGSITSMQGWRNKTTDWLKATYPQATFREVHAAIGGTGSDL
                     GVFRLGRDVLAQKPDLLFVEFATNDGGQAPEQIWRSMEGIVRQTWKHDPLTDIVFTYT
                     ITTAMTEDYLKGMCNRAASAMELLADHYNIPSINFGVRVAQLLKENKLIMSAREIETA
                     VPKESPDRDRLIRERIAADPRMLFANDGVHPRDEGHELYKAAIIAGLTQMRDLPAVDH
                     KASLANVFVQDHWEAAKMVPIRASMLNGNWKALPEDDPKQKSFGKRMGQIWMADQPGS
                     TLRFKFKGTVAKVYDLLGPDGGQVTVSVDGQTGAKPVPRFDSYCTYHRIATLGVFTGS
                     GAEVTREAVITLHPEQPDRSSISFRLKDPAKELAAPNFQGTRIWMSQIMLIGDLVE"
     gene            complement(12095..14512)
                     /locus_tag="PLZ74_00380"
     CDS             complement(12095..14512)
                     /locus_tag="PLZ74_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOR96810.1"
                     /translation="MKTQTFVRYLLCLALTLPVLTGCDRVPKGLEPEKLVLFSSETLL
                     GMPGTNMVNAIHVELLSKPVASLLGGAGTQHPVAGKRINAIPVDPAGGVRVSPPSQLT
                     DAGGSCKFDVTFGAVFGDHYLDLVCEEAPEIRKRLRFVCGVAVENVRQEVSAGDPLPK
                     PLRVRLVAPDGKPLADAGVHFTLTQQPGKHGRIGTSFARTDPDGIAEVSLKTDPHATG
                     TYEVRAEVADPKRGLFTRPVFLQAMSICLFRTLIGVLGGLALFIFGMTLMSDGLQQIA
                     GHRMKAVLAYVTRNRVAAIFAGTFITALIQSSSATTVMTVGFVNAGLLSLKQAIGVVF
                     GANIGTTVTGQMVSFNLDNLAFPAIILGVTGLLLCRKATLQGTARTILGFGLLFYGMT
                     LMSDELKSVSTFPSFVRFFHIFDCAPQSGQTMPIGAVLGAIGIGTLMTMLVQSSSATI
                     GLAIALANSGLLNIWTAVPIVLGDNIGTTITAVLASINANRTARQTAVAHSLFNVLGT
                     CVMVGLFYVPFHNVPCFLYLVNSVTAGNVFLGENIGRHVAMAHTLFNVTNVILMTPFI
                     GALAWLCEKIMPGKKVTSRVVHLEKNLLSSPPLALFCAMRALGDMTETAWNAAARSLR
                     GYKDGKPVDVNSIQEIEDEVDACQTGIMDYLVQLTRKDLSEKQAAAIPVLMHCVNDAE
                     RISDLALLIAKRSASLTSENTKFSDTALQELEALFDKASVIANLTRESLQDGRLLAKA
                     VEVVVNDLKAMARQSIQGHVERLQKGLCTPERGLVYVEVVGALENIVRHLENIAQRSD
                     ELTAAAT"
     gene            complement(14608..16572)
                     /locus_tag="PLZ74_00385"
     CDS             complement(14608..16572)
                     /locus_tag="PLZ74_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOR96811.1"
                     /translation="MLKISTLDAVIIFGFLFSVIAIGSAAARRSSRSAADFFLSGRSM
                     PWWLLGVSMVACTFSCDTPNLVTDIVRTQGVAGNWAWWAFLLTGMLTVFVYAKLWRRS
                     ALDTDLGFYEIRYSGRPAALLRGFRALYLGVFFNIMIMATVSLAAIKIGQVMFGLSPV
                     ATLLYASAGVGIYATLGGLTGSIWADFYQYSIAMVGAIFAAVYAVKSADVGGITSLKQ
                     LFSQPEVQTHMSMFPSGEGGLSALMTVLILPIAVQWWNVWYPGAEPGGGGYIAQRMLS
                     AKDEKNAVGATLLFNFLHYAVRPWPWIIVALVSLVHFPLTPPAEQAAATRWLADHPAQ
                     VGQYDTAKMQMPEPEREQVRQAKADAAGVGSLARAFPKVDDQFLRHDIAYPGMISTMP
                     KGWLGLIVASLIAAYMSTIATHLNWGSSYVVQDFYSRFINPNSTPRQAVAMGRVTMLA
                     LLVLSGLVALWMRNAKDSFDILLQIGAGTGLLYILRWFWWRINAWSEISAMVISFAVA
                     CFFQWGAAPLGLDAAMERAGLFAVMDYSAWKLVIGILITTIGWLTVTYLTPPENQDLL
                     ARFCTRIRAGGPGWRHIEEASSLPKSAEGWDVPTGLLCMMIGCLAVWSALFGVGNLLY
                     GRMFAGSFLLVVTVVATALLMRLVGKIRLS"
     gene            complement(16635..17498)
                     /locus_tag="PLZ74_00390"
     CDS             complement(16635..17498)
                     /locus_tag="PLZ74_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96812.1"
                     /translation="MRLLPNNVWFCLCLIGITLSGTAEVKRSARNPQRLTGQPAQRRS
                     NRSGQTASADKTLTQTAAPQNRVTRVVFMGPKSGWGFTKREVSYYSPEGKRLGTMPGG
                     TLFRYSDVKASSKNPMLLSRVRRNNAWDEPCLLDCTETATYEGDPETVDPEIVGNLHS
                     FFLLTAKIAAYKATLAEKAHAANPHFLAARDAQRAYQQSLAQADRLEAEAQTCTGARK
                     VKVLDALRALKYEQVRLQSEVTRTSAAFTKWNQTHPAVVPADDPTLRTLEREREAVRV
                     KVADLIPPDAS"
     gene            17628..17801
                     /locus_tag="PLZ74_00395"
     CDS             17628..17801
                     /locus_tag="PLZ74_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96813.1"
                     /translation="MLRQNKRPCPETRFARGVRALRKLTPGLTDRERAALWLVLALFV
                     LGALVRWLRMQDG"
     gene            complement(17867..23440)
                     /locus_tag="PLZ74_00400"
     CDS             complement(17867..23440)
                     /locus_tag="PLZ74_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96814.1"
                     /translation="MKRVSNDLCAALAVFLTCTGVASGVTNLLPVARILLFDDYADTS
                     LWTHEGPVITGLATRRALRSGTNHFHSAGWNIFNTRTGSARGQGSGLIVPPTLPSFNI
                     NTATNFAANLQNTLDAAIISPVFQEGIGTVYFEAVNNQAGRPTRITIWLTTNMTDQTS
                     GWPTNILLDAETQDLTYNWVQFGDDVVLDYSNSGEFYRYVRSLNYRGPAKLRWQRSGG
                     VSGGSALPPPEAENYGGVYTDAPGDGLDSFFTVIDNIRVSQPPSDVVVRKTACPFQPG
                     YPSVGTNMTVRCFTDNVDMNTPTDWTSRTNKVYYRWRYLDQASNDWTSVTLTNFFQGI
                     QGNNEGYEGVIPPHPRTGDLEYYFECSYAGYSYQSPDYTGEGHVYPSEAAAAGFSPRL
                     LGSRSQPFSIRIRPFVSPYGSISVVADEYPEPVTMELVADNTWQGMLPLSDAGTTNVS
                     WRLKGERRYLPVEVAYSTSVVHWAETLPGTINRLPYGGVCEETETPLTKTVTADRGAY
                     VMLTFDTETLKYMLVRAEYQNFNNWPALSDKFSESNGQTPKASFLNTFEAWPVSVEDT
                     YSQYFHLPVPTTNVFVREPFGTPQANWMAGHAAFSVERTLADENNKPAGYVNFRNQAL
                     RLQGGGCTLGLGYVHNTVQTRTDGIRDFAFKYRLAQTADKIDVAYDRTAFISSNYSAK
                     VTARSYTGHSPDAPSLSVIAYYQDADHFYEYRITQVPDTDNLSRDKRVTHQLYRWING
                     TPTLLATSGTYDTGNAGDIVGFEHAMEIRIFNQPNSTLIRCYYYPSSSAVLRIDHTDN
                     SDSRLTYGTYGVLSEDCGAGFSNFIIQPTTTGATLTGSAITVLSDTNAFNTDKNNWSA
                     PSGRFVLDGDRSPKGIHAVIPTQKLGVYLQSSAQDSTAEPAAPGTTAWKLFQEITVSG
                     YGYKTNAVTVKDWKTQFLLLQVMGVAGSKADVVVDELKITSWRGEQFGSGNDDAWLAT
                     EAWAVSNNAANGHVIQLKHTRADPNADQAVRSILLENGMGMLEFDYRVLRAPAQLTVQ
                     YATERENDIWIPVHTYAISNVMSEWDHISGYVGITNPGFLRVINDRSGGCSNAWVEID
                     NVRAWEEPVVKDTSWRAYNVKVTQTDKQRIMLDGEEACLLNNSTTQDAEPIQDQNVPF
                     VQSPVLPYGLGELSFKTRAYTNTFPSSLYIYATTNGWNAPTNLWFEIAAFTQITNRFY
                     ETVTVKPVDGRDYDAIKFISPLGGQRACIEDIAVTEPVFPGFDIENVQVLCRNGDSYD
                     DDPPRHQPINSDHVGIQAELVNIRLSPSNIQMYVDYYIGTNVWGVENWPAAQVVTLPM
                     EQMGNSTTYQTLPSQDLPMLDSDTIVQYRVRATYLGGIPLTAEQKTFENPPWYFPVDF
                     NAVYASQGWSPYYIVYGVPLKTVWINEVNAAEHVEENGVPQYGFYDNPYIEIAVPVGV
                     DLAGWSIDLVMNDRDMHTITLPEGLPEQTAVTNGYAFFVIGEKFPDPNRTPVPLPKLD
                     YGEPGFYWYIPCIYAGGLRLKRPMGMYEHVIAYDDPSQGSGPEFDGELWAAEDPERRF
                     VYVGPETHGGSLNVTNGTGVTRADWTFPLYWTPGGLNPGQSVAFPELGVSNHWIVSTL
                     NALNGTQNGLRTQCHTFRTRNGANTNILYQADNWYRLYSVKVNQVEQLPPGEGLQNYL
                     LPLTNVSANLNVEAHFALRQDIADLEISDDMLNWLLGFGDNNTPLASSYFFGNGRLMS
                     LTELYWLNADPTVTNRVDGSIVRFTRDAETNYYVTASLAVNGQNCTCLLSQAVMKLEM
                     TPSLTIPDWRIAGQYRLMPDSFDTNHQCRILLPNPYIYSFPYWDPAQAFFRWVIEEQH
                     PNIGVLPLVNTPPEP"
     gene            complement(23508..24383)
                     /locus_tag="PLZ74_00405"
     CDS             complement(23508..24383)
                     /locus_tag="PLZ74_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOR96815.1"
                     /translation="MLFVRKTGFTLIELLVVISILGILAAALTTQVGRARSMGQAIRC
                     KTNLRNLAQASLSYAVETKHMPWAGSFQVRYPSRDGNRYVPKYHEHRGWVGWVGSGSW
                     PNKDIQNMEISCFHGNNAYYSLTNGSLWTFVGKDVSTFLCDTHKKAAARTGLEKIHRS
                     YVMNAYFGYEHKAPPKTADVRRWLESLSSSGNAASRLLFAELPARSIVSSGEGADSVL
                     QAEIKNYANPGSPEMIGFNHKVGKRYVAHVAYADGHVDALIEPEGGNKDDCIELTRQL
                     CNGEDITEELRDRMR"
     gene            24546..26303
                     /locus_tag="PLZ74_00410"
     CDS             24546..26303
                     /locus_tag="PLZ74_00410"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOR96816.1"
                     /translation="MRWSRALLNTLRDDPQEAEINSHKLMVRAGLIRKVAGGLYTFLP
                     LGLRALHKVTRIIREEMDRAGAQEILMPALQPAELWERSGRLAAMGPGMFRLKDRAER
                     LMTLGPTHEEVVTSLLAGEVSSYRQLPCTVYQVQTKFRDEIRPRFGLMRAKEFIMKDA
                     YSFDTSMEAADRSYQAMYDAYTRIFQRCGLRVKPVEADTGDIGGSWSHEFMVLADSGE
                     DGIVDCAACGYAANQERAERMGKPAAEAACPPLSEVPTPGVRTIEDLTAFFDCAPDRF
                     IKTLIYLADGKPVAALVPGDRDVNEHKLKRLLNASKIELADEETIFRVTGAPVGFAGP
                     AGLSIPVYADQSLEGAEDRVTGANRADTHYRQVSLARDARVTAYGDLTVVGSGDLCPR
                     CGKPLQLRRGIEVGHVFKLGTKYSESFDAVYLNEKGQSEQMVMGCYGIGVTRTLQAVI
                     EQSHDADGIIWPVSVAPFAVSLLLLDPRDERVYDVVAQLEAELEACGIDVFVDDREER
                     PGVKFKDADLIGCPVRVVAGAKGLAKGGVEIKLRTGKESEIVPVEQAAAAIADRVKAL
                     TSALSAKADEPAACEAGNG"
     gene            26349..26675
                     /locus_tag="PLZ74_00415"
     CDS             26349..26675
                     /locus_tag="PLZ74_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR96817.1"
                     /translation="MKPSKYDKAPERVALKKRDLVNRIAKDTGLTQLQVLDIVQRTLD
                     GIVDALEAGQHVEFREFGVFSVVKRKARIGRNPNQPADVVTIPVRYAVKFKPGKRMRD
                     MLADKV"
     gene            26707..27984
                     /gene="hisS"
                     /locus_tag="PLZ74_00420"
     CDS             26707..27984
                     /gene="hisS"
                     /locus_tag="PLZ74_00420"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOR96818.1"
                     /translation="MNVPASFSFDPPRGMRDFYPEDMFWRNRIFDAWRQAALAFGFQP
                     YDACVVESLDLLQRKSGEEVSEQIYHFEDKSGRRLALRPEMTPTLARLIAARQGQLVF
                     PVKWFTVAQCFRYERMTRGRKREHYQWNLDVVGEGQVSAEVEVLAAAAEGLRRMGLPE
                     QAYRIHVSNRALLAELLAVSGIAPEHHAAVFLALDKRGKISDDEIAGLLRAEGLKDPA
                     VEQTFALLGIQTLDQAAERVGPDSAAVAALRELFALAGVYGIADRLMFDIAVIRGLAY
                     YTGIVFEAFDSARKFRAIFGGGRYDNLLTTIGGAPTPAVGLGFGDVVIAEVLKDLLSE
                     TAATEKNGVIIGYMFPEQRESAMRLARSLREAGERVDLHLARQKPKTFFAKAGAGSAA
                     DAIFIGPDDVARGTVRRKHLATREETVIPLPEA"
     gene            complement(28073..28912)
                     /locus_tag="PLZ74_00425"
     CDS             complement(28073..28912)
                     /locus_tag="PLZ74_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOR96819.1"
                     /translation="MKFTRRSFLRHSAATLSLTAIPSPGAHADKPKIRVGSCTLRLDQ
                     ARQAGLDGVQLWAGGAADELDIYKADTRARLKTQIRETGLPICSLMLGLLNSYPLATD
                     PRAPAWLRQSIEAAQDLGVPNILVAFFGKGDLQADRKIKEPEFASACRRIRDAAPRAH
                     DAGVTLSIENLLSAEQNLRMLDTIGHEAVSLYYDVYNTGKSMKYDSPAEIRLLKGRIT
                     QIHYKNGPRYLDEDRPYFEAVTAAIREIRYSGWIVLETSSPSKDGVADGKRNGDFVRS
                     LFA"
     gene            complement(28939..29934)
                     /locus_tag="PLZ74_00430"
     CDS             complement(28939..29934)
                     /locus_tag="PLZ74_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOR96820.1"
                     /translation="MHVKLDIPGFVDLQVNGFVGVDFSGPDLTEESFKLACHTLLERG
                     TAAFLPTVITGTPEQTRRNLERMASVLKKDAVLARHIPGFHLEGPFISSEPGAVGAHD
                     PAAVRPPDCAYFDRLCQWAGDRIRLLTIAAEIPGAEVLTRHAVARGILVSCGHQLADA
                     AALARLVDAGASALTHLGNGCPNQAHRHDNPLLTGLAEERLQILFIPDGHHLPAHVLK
                     VFARAAGARRLIATTDASPAAGLPPGTYNVLGNQAVLEPDGRLHNPEKQCLVGSSATL
                     IACMNVLHRLNLFTLEELLQIGFRNPLALIRVESREVPTCRYRITFSPKTGFVLR"
     gene            30082..30522
                     /locus_tag="PLZ74_00435"
     CDS             30082..30522
                     /locus_tag="PLZ74_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96821.1"
                     /translation="MDWRAELSGILGGRARTTRAEEENAVFEAFLDTVAIPALSEIAE
                     ELVQKHERDAQVRRAPASATLSVRVGEMEEITFRVMKQFVQNGILPRAEVRLNRGQRL
                     VKYESMFRADPQTYPITEITQDEVIACFIKYYRMVMDRNPTAAE"
     gene            30592..32022
                     /locus_tag="PLZ74_00440"
     CDS             30592..32022
                     /locus_tag="PLZ74_00440"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOR96822.1"
                     /translation="MMKIPLPKIIQGGMGVGVSHWRLAQAVSKLGQLGVVSGTGLDQV
                     LARRLQDGDLGGDVRRALSHFPFPQMAQRVLDALFVPGGRPADQPYQAATKHTIKSNR
                     WVDELCVVSNFVEVFLAREGHSNPVGINYLEKIQFPHLPSVYGAMLAGAAVMIVGAGI
                     PVEFPGVLDALSRHEAATYTVRVHGATPETDCQRVFDPALFFEDGCTPPRLLRPDFLP
                     IISSDSLATMLLRRASGSVEGFVVEGPTAGGHNAPPRGPLQLTPDGQPIYGPRDVVKL
                     ESMRKIGMPFWLGGGYGSPEALRTALAEGAVGVQVGTPFALCEESGLEPKMRHALIHL
                     ALAGDGKVFTDPLASPTGFPFKVARLEGTLSEPEVYTARRRVCDLGYLREAYVQADGV
                     IGYRCPAEPVEAYTAKGGSAEDTVGRKCICNGLFGNIELGQVRADGTREPGIVTLGDD
                     YLRIGRFCTLEQPDYSASHVVRVLLG"
     gene            complement(32073..33179)
                     /locus_tag="PLZ74_00445"
     CDS             complement(32073..33179)
                     /locus_tag="PLZ74_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOR96823.1"
                     /translation="MTPTLITTAADLADLNTRLLRAEAVALDTEFFWERTFYPVLGLV
                     QLATPDGCWLVDAVCLKDLSALAPLIASPSVTKVLHDAPLDLTILARATGAVPRSVFD
                     TRLAAGFAGLSATLSLQALLQEVLGVTIAKGETRSNWLRRPLSENQIRYAAEDVVHLL
                     PLREALLARCAGKRSRNWLDQELARLDDPAHYEESPPEERFRRVKGAARLNRRHLALL
                     REVAGWREREARRRDWPRNHVLPDPVMVTIALRGPADVRALMAVPGFPSRMPAKLVGQ
                     ILAAVERGKALPESACPDPSDRNPATARALKEPTDRLLAHLRIACEKEGIDPAWIASR
                     NEAESLVQSLARGDASAHPLMRDWRQTLLAGFTL"
     gene            complement(33190..34575)
                     /gene="lpdA"
                     /locus_tag="PLZ74_00450"
     CDS             complement(33190..34575)
                     /gene="lpdA"
                     /locus_tag="PLZ74_00450"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOR96824.1"
                     /translation="MQDFDIIVIGAGPGGYPAAIRAAQLGKKTAIIEKEALGGTCLNW
                     GCIPTKTLIASAERYHHATQSATFGVTAKGVTFDYAAMTARKAEIVDKLSGGVRMLLQ
                     SNGVELINGTARFEGPNRLSVLQPDGTTRWLSAVSIIIAAGSDSAMPAFLPKDKRVMD
                     SRAFLDLTELPKKLLILGGGVIGCEFACMAAALGADVTVVEMLEDIVTVLDRDVRRVL
                     RKRMETLGITLLTGTPLTDIAVKKNKVTGTFKDQRVEGDVLLVAVGRKPNTALLDLAR
                     AGIKTDERGTIPVNAQGRTNVASVFAVGDLVTGSIQLAHAATQQGVTAAEAAAGLKSK
                     VERICPACIFTSPEIGLAGLTENMARQRGIAVKTAVFPFSALGKAMTAGETDGFVKWV
                     CDPVTGQVLGAQAVGCHATELIAEAALAIRNELTVHEIGNTIHCHPTLSEAWMEAAHA
                     FHDACIHLPKR"
     gene            complement(34633..36018)
                     /locus_tag="PLZ74_00455"
     CDS             complement(34633..36018)
                     /locus_tag="PLZ74_00455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOR96825.1"
                     /translation="MAQAVIFPKLGQTMEEGAIVQWLKQEGDPVVKGDILFEIETDKA
                     NLEVESFFEGVLLKIYVREGVTVPVNTVVGYIGAPGEKVPDQAPKPEAPAPAPKPVAS
                     PSPTPAAARVPVAEPAAAPAPVPARAPVPAPPSHPAPQPAKVPVPAARLVISPRAKTL
                     AQSACINPARITGTGPNGRIVEKDVRAYLAASGYDALRITPAAKRLAAEKSIDILTVR
                     GSGDSGRIMVEDIERAIRRKPVALSRMRQVIARRLVESKQTIPHFYVTVKADITDLMV
                     FRSDLKARGLNYSVNDFVLEAVVLSLEEIPIVNSVTDGQTVSWRGDVDLGMAVSVDNG
                     LVVPVIRAAQTLTFSELSAQAKALAEKARAGRLTPDEMTGSSFSVSNMGMLNVDQFTA
                     IINPGEAGILAVSSGRQEPAVVNGEVRIRSRMAMTLSVDHRIVDGAVGAQFANAVRSK
                     LENIELWKSLV"
     gene            36233..37354
                     /locus_tag="PLZ74_00460"
     CDS             36233..37354
                     /locus_tag="PLZ74_00460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR96826.1"
                     /translation="MITVLHVLEATEGGTRRHLRDLVGALEPLGFRCALAVSCRRDPA
                     FRRDVAFYLSRGLTVAELPMRRGLAPIADVAALLRLIGCVRRVRPSVIHAHSAKGGLL
                     ARAAGAVCRVPVVYTPHVFPFLMACDPRMRRVYRLVERGLRCMTAAVIAVSEEERREA
                     AALGYAASRIYLIPNGVDGKVPGEVTVRKDGELRVGFFGRLTAQKGPSVLLEAVADVV
                     AHLPQVRFVFYGDGTEADTLRARAEVLKLNDAVRFLGSVAQGEVVAHMRQTDVIGIPS
                     RWEGCPYVVLEAFQAGVPVVAAAVGGVPDLIRDGVTGVLVEPDNPEALCDALLSLLRD
                     PQKRRRIAEQARNELARYRLDAMAAAVADVYRRVTQDLP"
     gene            complement(37362..37580)
                     /locus_tag="PLZ74_00465"
     CDS             complement(37362..37580)
                     /locus_tag="PLZ74_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96827.1"
                     /translation="MGTGRTLNKAPRTRPIKSQAEKRRRLKAQRARLVKIGVDDSVVA
                     KMQPEEVRKLVQHPAKVKKSLATQNRSA"
     gene            37788..>38053
                     /locus_tag="PLZ74_00470"
     CDS             37788..>38053
                     /locus_tag="PLZ74_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOR96828.1"
                     /translation="MSVSVGFISLGCSKNLVDSQVMAGYLKAGAIALAPSPEEADVVL
                     INTCAFIESAREEAAGAILRACAHKERGGCRAVVVAGCMVQRYRS"
BASE COUNT         7264 a        10530 c        12583 g         7676 t
ORIGIN      
        1 gttacgtccg cttctacagc aagggcaaca cctcgaacga gatgaaccac tacacggaga
       61 ttgaagtgta cggcaagccc tgagcgggag taggagttgg gatttaggat ttaggattta
      121 ggatttagga tttaggagtt aggagttagg atttaggatt taggagttga tgtttctgaa
      181 tccgaaacct gacttcggca acaagagcgg cgtggcgggc aaacgggtcc gtccgccgcc
      241 tgttttgttg cgtaagaaat tgacgcattg ggccggttcc tgatatgttg tccgaaacca
      301 tcaggatggg tttaggattc aggaggacga aacgatgaga atgaggattt tcgcgacggt
      361 atcggtgatc ggatgcttgt ggctgacggg gtgcaagcct gcgggagcgc cgacaacgga
      421 ttccgtaccg gatgcaggat ccggcactgc ggcgcaggtg ctggagttga atgacatgac
      481 ttttacggcg cagaccgcgc agggcgtggt gctggttgat ttctgggcac cctggtgcgg
      541 gccctgccgt acccaggggc cgatcatcga gcaggcggcc aagctgttgg gggattctgc
      601 aaaggtggct aaggtcaatg tggatgacgc gcagcaaacg gcaaagcgtt ttggggttca
      661 gggcattccg acgctgatcg tattcaagga tggcaagccg ggcaaacggt ttgtgggcgt
      721 gactcaggct gcggtgctgg tcgaggcggt gaaagaggcg ctgtgatatt taggatgtga
      781 cgtgctcttt ccgcctttgc gccttcggga tttttgacct gacagactcc cgccggagcc
      841 cgtatactgc ataccaatgg gttttatgcc ggaggatttg cggggggtgt gtgcgtgagt
      901 gagacggcca agttgttcct gatcgatgtg atgccgctgc tgtaccgcgg ccatttcgtg
      961 tttctcaaat ccccgcgcgt gacgagtggc ggggtcaata cctccgcgtt gaccgggttt
     1021 gccaactgtc tgttgcagat tctcaacgag cacaccccca cgcatgttgc gttggtgctg
     1081 gattcgtcca cgccgacttt ccggcatgag gcatatcctc tctacaaggc ccaacggcag
     1141 aaaatgccgg aggacctggc ggccgcgatc cctatgtcga tcgagttggc cgaggcgttg
     1201 cggattccga tcttgcgcgt ggacgggttt gaggcggatg acgtgatggg gactctggcc
     1261 gcgcatgccg caacgaaacc cggctggcag acttatctgg ccacgcccga caaggacgtg
     1321 gcgcaactgg tcggcccggc gacgttcctg ttccgtcccg gcaagggggg ggcgccagcg
     1381 gaaatttacg atgaggcggc ggtatgccga cattggggtg tgagtgcccc tagccagatg
     1441 atcgacctcc taggcttggc gggggatgcc tcagacaaca ttccgggcat tcccggtgtg
     1501 ggtgagaaaa cagcgaccgc actgttggcg cagttcggcg atttggaaaa cgtgcttgcg
     1561 catgccgcgg aactcaaggg gaaactcgcg gagaaagtgg cggccggggc ggcgagcgcg
     1621 cgacagagtc gtgagttggc agccatccgg acggatgtgc ccctgacgat ccgcctcgac
     1681 gacctcgcgt gccgggatcc cgatgaggcg cgtttgcgcg cggtgctgaa gaaatatgag
     1741 ttggcggcgc ttggcaaacg gctgctgggc gaggggttta cggacggcac cggaggtgtt
     1801 gcggcatccg gcgcctacca aacgttggcc gatgtcccgc atacctatgt ctgcgtcacg
     1861 gacgaggagc ggatgcggga attgctacgg gcgctggagg aggcacccga gtgggcgttc
     1921 gacacggaaa ccacggggtt gaacccgcgt cacgaccggc tggtgggcct ctccttcgcg
     1981 accgcgccgg gacgggcatg gtacgtgccc gtgcccgaaa cgcatgaggc ctgccgcgcg
     2041 gcgctgcacc gtttcgcgcc ctatttcgat gacccggcta cgatcaagat cgggcataac
     2101 gcgaaattcg atctcacggt cctgcgtcaa tatggcatca ctctgcgcgg tgaattgcat
     2161 gacaccatgc tcgcgcatta tgtgatcgac gcatcggatc ggcacggcat ggaccatctc
     2221 gcacggcagt atctgcatta caatccgatt ccgatcaccg cgctgatcgg cgaaaagaag
     2281 cggggcacgg tgcaggggaa catgggtgac cgggctcccg aggagatctg cgactatgcg
     2341 gccgaggatg cggacatcac gctgcggctg gaccggctgt tgcgtcagga ggcgcaggaa
     2401 acgggatgcc tgcgcgcgtt gaccgagtgc gaggagccgc tggtcggcgt gctgttggat
     2461 atggagtccg aggggatccg catcaacgaa caggcattac gggaatacgg gcgcgaactg
     2521 gatcgcgaga tattggattt ggagatccgt attcgtgaat tcgggggcgg tcccttcaac
     2581 cccgcttcgc ccaagcaact cggcgaggtt ttgttcgacc acctcaagct tgatccggac
     2641 gccgcgcgca cggcatccgg tcagtacgct accagcgaag aagtgctgct caagctccgg
     2701 gaccgtcatc cgattgtgcc gctgattctg gagtaccgga tgtgcggaaa actcaaatcc
     2761 acatatgttg acaaactgcc cgactgcatc gatccggaga ccggacgcat tcacacctcg
     2821 ttctcgcagg ctctgaccga aaccggccgt ctctcctcct ccgatccgaa tctgcagaac
     2881 atccccatcc gaaccgagcg cggtcagcgc atccgttccg cttttgtggc gcgcgatgcg
     2941 gatcatgtgc tgctctccgc cgactactcg cagatcgagt tgcgcgtgat ggcggcgctc
     3001 agtaaggatc cgggaatgat cgaggcgttc cggcgcggcg cggatattca tcgcgagacc
     3061 gcctcgcgtg tgtacggcgt gccccccgac gaggtcacgc cggagatgcg ttccgtctgc
     3121 aaaatggtca acttcggcat catttacggt atttccgcgt tcggcttggg tcagcggctg
     3181 gatgtgccgc gcaaacaggc ggcagagctg atcgaaacct atttcaccac ataccccggc
     3241 gtgaaaacgt acatggatca ggcgattgcc gaggcgcgcg cgaaaggata tgcgcagacc
     3301 ctcttggggc ggcggcggtt tttgcgtgat atcaattcgc gcaacgccac ctcgcgtcaa
     3361 tcggccgaac gtaacgcgat caacaccccg gtgcagggca cggcggcgga tctgatcaag
     3421 ctggcaatgg tgcgggtaca ccgcgaattg gccgaacgcg gcctcaagac tcggatggtg
     3481 ttgcagatcc acgacgaact gctgttcgac gcgccccgcg gggaagtcga aacggtccgc
     3541 gagctggtca cacaggccat gaccacggta tgggagatcg gtgtgccgct ggaggtgagc
     3601 gtcgggacgg gggaaacttg gctggaggct cattgagcaa cgccttccgg gatattggcg
     3661 cagacgcgcg ggacgtatgg ggaatcaggc atgaatgaga aggaagggca ggaagtgacg
     3721 cgcaggaacg cgctggcctg gttgttgcgg gttggcgcgg gcgcggcgat aaccgccgcg
     3781 gccgtccaag ccacacggcg ggcttcgggc aagcggacgg tctggcagat cgatccgcgc
     3841 aagtgcattc agtgcgggcg ttgcgaaacc gaatgcgtgc tccacccgtc ggcggtgaag
     3901 tgtgttcacg ggtacgcgat gtgcgggtat tgcaaactct gttttggcta tttccaaccg
     3961 gatgccgcgc gtcttgatga ggcggcggag aaccagattt gcccgaccgg tgcgatccgc
     4021 cgccagttca ttgaatcacc ctacttcgag tatcacatcg atgaagcgcg ttgcacgggc
     4081 tgcgccctct gcgtgaaggg gtgcacacat ttcggcaacg ggtcgctgta tctgcagatc
     4141 cggcatgacc ggtgtgtcgg atgcaacgcc tgccgtatcg cggaggcgtg tccggctcag
     4201 gctattatgc gggtgtcggc cgacacccca tacctgctga aagaaaaact ggggccgtcc
     4261 ggcccaaagg aatcggacgg atgaagcggc cggggtgcat ccggaaacgg gcgggtctgg
     4321 cgggtgtgct gttgccgttg gttacctggg cggccgatcc gcgttttcca caaccgcaat
     4381 tcgaatcggg gcatacgctg ccgcagacgc cgcatccggc ctttgtggag caggtgcctg
     4441 cttggatgga tgtgctgttg ctcgcgttgg ctttgggcat agcggtctgg ctggtgcggc
     4501 gattgcggtc gcgacgcggc gtattgctct tttcgctgct ctgcttgggg tggtttggtt
     4561 ttgtgcggcg cggatgcgtc tgtccggtcg gggcggtgca aaatgtggcg gaggccatgt
     4621 ggaatggcgg cggcttgccg tggtttgtgg ccgcaatctt cgttttgccg ctgttcgcga
     4681 cgcttctgta tggccgggtg ttttgtggcg cggtatgccc gttaggcgcg ttacaggatc
     4741 ttttcatcat ccggccgcag cgggtacccc gagccatgga tgcggtgttg cggttggtgc
     4801 cgctcggggt gcttgcgctg ggtgtggtgg ctgcggtcaa cggtgcgggg tatctgatct
     4861 gccaatggga tccctttgtg gggctgttcc ggcgcagcgc gccgatgacg gtgatgttaa
     4921 ccgggatggc cgttctgttc gtggggatgg tggtggcgcg tccgtactgc cgttatttct
     4981 gcccatacgg tgtgctgctg gagttttgcg cggggttggc gtggcgtccg gtgacgatcc
     5041 cctcgcggac atgtgtcaac tgccggttat gtgtcggcac gtgtccggtt ggcgcggtca
     5101 cggcaccgcg ggaggtgacg tccggtgcgg aacggaagcg gcttttccgg cggtttgtga
     5161 cgttggcggt gctgatgccg ctcggtgtgc ttttatgcgc aagtacaggc tggctgaccg
     5221 gtgcttgggt cgcacgggcg catcctgcgg tgaggatggc ggaagtgctc gcgacggtac
     5281 cgcaggaggc ggcggatcag catcccgaac tcgaggcgtt ccgacgcggg ggcgcgtcgc
     5341 gtgatgaggc tgaggcatac gctgctgaag cagtgcggcg tttccgagcg gggggggccg
     5401 gtgccggcgt atttgtgggg gtggtgatgg gcctgcggct tttggggatg acacggctgc
     5461 gacggcgccc gctgcatgag gcggaccgga tgcggtgtgt tgcctgcggc cgctgttggg
     5521 acgcgtgccc cctgaaccgg tctgttgccg gaacctcagg atcgacgtct gacggccctt
     5581 cagtgggttg aggtcagggc cggttccgga aaatccggta gcggtacgcc gatacgtggc
     5641 acggcactca acaggagccg tgtgtacgga tgttccggtg tgttcagcac ctgttcggcc
     5701 gggccggttt cgacgatccg gcctcggttc atgaccgcga tgcggtcggc gatgtgttgt
     5761 accacgctga tgtcatgcgt gatgaacagg taggagagtc cgtgtttcgc cttcagatct
     5821 tcgaggaggt tcaacacttg tgcccgcacc gaaagatcca acgcgctgac cgcctcgtcg
     5881 cagatgagca gttgtggccc gagggcgagt gcccgggcga tgcacagacg ctggcgctgc
     5941 ccgccggaaa attcatgcgg ataacggtcg aggatttctt ccggcaggcc gacatcagcg
     6001 agcaggcgta ccgccgcgtc gcgacgggtt tcaggcgtca ccagccggtg cgccaacatg
     6061 gcttcggtga gcaggtcgag cacggtgtga cgtgggttga gggaggcgta tggatcttgg
     6121 aacacgactt gcacggtgcg gcggtaggtg cggagatccg cgccgcgaag cgtccggatg
     6181 tcccggccgt tcagcaggag ggtcccggcc cgcggtttct ccaggcgcag gatagtacgc
     6241 gcgagagtcg atttgccgca acccgattct ccgacgatgc ccagagtctc accccgcatc
     6301 agatccagat ccacaccgtc caccgcgcgg atatgcccga cggtacgggc gaagacccct
     6361 tttttgacgg ggtaccaagt tttcagcccc cgaatccgca ggagcggcat atccctgtcc
     6421 gaattggcca tgccggtata tagcataccg gcatggcgga cggacaggat aaaaggcgaa
     6481 tgtcggtgaa acccgcagcg gattatgagg attgggagac aaacgaaccg gggtgaccag
     6541 aaactccggt ttccttccgg gaagcggatg tgttatgctt ctttttttca aacccaatga
     6601 ggaatagccg gacatgaaca agaagacgtt gcgtgatgtg gacctgaagg gcaagcgcgt
     6661 ggtgatgcgc gtggatttca acgtgccgat caaggaaggc gtgatcaagg acgatacccg
     6721 tattcggggt gccttggcgt cgatccgtta cgtgctggag cagggcggga gtttggtgct
     6781 gatgagccac ctcggccggc ctaagggcaa agggtatgag gaggagttct cgctgaagcc
     6841 ggtggctgaa caccttgccg ggcttttggg taagcccgtg actttcgcgc cggattgcgc
     6901 caacgccgac gcggaagtct cggcgttgca gcccgggggc gtcgtgatgc tggagaacac
     6961 gcgtttttac aaagccgagg acggcaaggt caaaaagacc gatgagatga gcgatgagga
     7021 ggttgcccgg aagaaagccg agttgaaagc caagcgcgag gagctggcca aaaaactcgc
     7081 ctcctacggt gacctcttct gcaacgacgc cttcggtacc gcccaccgcg cccatgcctc
     7141 cacagccgtg atctgtaagt tcattccgga gagtgtggcg ggtttcttga tggagaagga
     7201 gatcgagtat ttaggcaacg cggttgagaa gccgacccgt ccgtttgtgg cgattctcgg
     7261 cggggccaaa gtttcagaca agctggcggt ggtcaagaac ttgctggata aggtcgacac
     7321 gctgatcatc ggcggcggca tggcctacac gttcctgaag gcgcaaggcc acgatgtcgg
     7381 cggttcgctg tgcgaactcg atcaattggc ctatgccaac cagatgatcg agaaagcgaa
     7441 ggccaacggc aaacggctct tgctgccggt ggacaacgtc gtggccgata agtttgatgc
     7501 cgaggccaat acgcaggttg tcggcaatga cattccgtcg ggctggatgg cgctggatat
     7561 cggtcctcag accacggagc tgtatgcgac cgcgatcaag ggtgccaaga ctgtggtctg
     7621 gaacggtccc atgggctgtt tcgagatggc caagtttgcg ggcggcacca ccggcgtttg
     7681 taaagccgtg gcggagagcg gcgcggtttc gatcatcggc ggtggcgaca gcgtgagcgc
     7741 ggtcaacagg agcggtctgg ccgataagat gacgcacatc tccacgggtg gcggtgcttc
     7801 gctcgaattc ctcgagggga aggaactgcc cggtgtggcg gcgttgagcg acaagtaaga
     7861 cgcttcaggt gttgaaaggg aagcagccgg acgcgggaat cacgatcggc tgcttttttt
     7921 tgttccgata agtttgtcag actttggttc cagcggaaga agctgattcc gcaggtgcct
     7981 gtcagtaatg cgttagcgga aaccgtttta cggagcggga ggcgtcaagg cggatatcgt
     8041 acgctcggag aagatcacgc gccaggaaac ctgtccgccg ctgccgctga tgaatgcgag
     8101 gcgtacgagg ccctcttcgg atttcggttc gaagagtgtg gtgcgggggg cgtccggtgt
     8161 gttggcgtgg gcgtaggcgt catccggacg ggtgacttgg ccttcaccgg gcgcgatcgt
     8221 cagttcatat ccattcggac cggtgatcgc tgcggaaagt actgtgccga aggatgctga
     8281 ggcgtccgca tgcgtgagga tgcgcaattc gtatcggtca tcggccacgg tgtcactgac
     8341 gccggagagc gtgcgcgaga ccggatccca agttacggcg cgcacatccg taatgccctg
     8401 cgtgatgtgt cgcgaggtgc tgatgagttg aggccggtca cgtatcggac acagcgcgag
     8461 gaccgcgctg gattgcggcg ggagggtgag cggtagactg tgccggaagg ggggcgtgag
     8521 ggtgttgccc cagtattcga acccgatcca gggggaacgg gtttcgagac cgagtgaggc
     8581 gagcggcacg ttgaaggttt tgtttgtgcc gtcccagtta aagagcccgg cgacctgacg
     8641 ttgcggccgt tccgctgtcg cgccatctgt caccagccag atgccgggga tccggttttc
     8701 gaagagatcg gcaggccggg ctacggcctg atgcgagggc atggtgcgcc ggagcagatc
     8761 cagacggtcc ggcggcaacc ccgcatagtg ttcgctgctg ctgttcatct ggccgctcag
     8821 agtgacccac gcggcaaggg cttgcgcttg gtggatcggc aaggatggtc tcacatacag
     8881 cgggtccgga tcgttatacc acacgcggcc gtgcagaaag tagttccatg cgccgtattc
     8941 tggaccgcgc atcatggcgg accaattggc cccgttgtcc ggcccgatgc gcatcgcatc
     9001 gaccagaccg aaggccgcgc ccgcgctgcg catgttttgc ggtgtgcagc agccgagcag
     9061 gaacacatcg cggcctgcgg tgtcgcgcac gattttgaga ctgttacgca tcatctggat
     9121 cggggtgacg gaggggtcgg tgagtatcgc ctgaccgatc tcatcctcgc ggaaggtatt
     9181 gcacacgtaa ttcaggttga tcgccgcgcc ggcgtagagc gcgtccattt tcagataacg
     9241 gtaaccccac tccgcagtga gggtgcggat catgtcgcgc agataagcct gcgtatcgcg
     9301 gcgggtcagg tcgaaacaag tcccgcccca acgggtgtca aagggcgatc cgtctgatcg
     9361 tttggcgaac cacgccgtct tgtcagcgaa ccaggggtct tcgcgtgaac ctgcgaaggg
     9421 gatcagccag attccggcgg tcatcccggc ggcgcggatg gtgtcggaca tggctttcat
     9481 gcctgcggga taaggaccgt cggggcgatg gcggatgaag actttcttgg ggccgttggt
     9541 cgattgtccg ctctgccacc cgtcatcgat ctgcatcacg cggaggccga acggcttgag
     9601 cgtttgtgcc gcatacgcgg tccggtcggt cattacccgt tcgttggagg ccctgtccac
     9661 ataccaggta cagtggacgg tcggcatggg cggcaggcgg atcgcataga tccgggcgat
     9721 ggcgtccgca taagcctcga gaccgaaacg cgcgtcgtca aacaacccga gggcgaaaag
     9781 ttccagatct tcttcgcgat ctgcattggg tgcgaggcga ccgtagtcga gatgcggcaa
     9841 cagcgtgacg cattcctccg caacagccat gcgcacgata ccgctgccgc ggtctgttgt
     9901 gagccagccc gcaaccacgc cacgccgcgt ggcgggatcg gccacggcag tccacatgta
     9961 gctgccggga ttgcgtttcg gctggagcag ccctccggta ccgaggctgg tcagttcttc
    10021 ggcgggccgg tccagcgcga gccgcaccgc agggtaggcg attcgattaa ccgggttcgt
    10081 cgtgttcgtg ccgcgccaag atgtgcggag cacggcaaaa ggcagatccg caaagaggat
    10141 cactcggttg cggctcccgt gggggaagtc gaattccaac gcctcaccag cacccagacg
    10201 gggctcgttc agacggaccg tacggacggc agacggtttc tgcgtagcgg catcgaatcg
    10261 ggcaaagggt ttggactcgc cgggaaccca caaggtcacc tcgccggaag cctcccggta
    10321 ttggagaaga acggcgcggt tctgaagggt cagatcggcg gcggaaacca atcctgcggc
    10381 aaccagcagc aggcatctga gtaatgttgg tcgggaggtc gtcattcggg tcctttccgg
    10441 catggagaat cacatgcttc gcagggtaac agaggcgcgg gaccgattca agttcatgcg
    10501 gctgcgcaca gcgcgacggc ttggtgtctc tctcatgcaa agtacctgtt tgcgcgtgtc
    10561 ggatcaagcc cgtatccgga taagcgtaac atctgccgtg cggttgtttg cgatcccccg
    10621 ccatcaacga ttgcgcaaac tgcgctttac ggacgggctc cgatggggta tggtgaaatt
    10681 ccggttgaat atcggatcac aaactacaag ggggaaccat gaggctaacg ggaaggggat
    10741 tggggacgat gtggttggcg gcggtgtgtg cggcggcggg gattgtgtcg gccgcgccgg
    10801 aataccggca ggtggcggca gaggaggtcc ggccgcgcgg cggaatcggt aatgtgatgc
    10861 ggaagatccg tgcgggcggt gaggtgaccg tcgcctatct cggtggttcg atcacctcga
    10921 tgcagggttg gcgcaacaaa accaccgatt ggctcaaggc gacgtacccg caggcgacgt
    10981 tccgtgaggt gcatgcggcg atcggcggca ccggaagcga tctgggtgtg tttcggttgg
    11041 gtcgcgatgt gctggcgcag aagcccgacc tgctctttgt ggagtttgcg accaatgacg
    11101 gggggcaggc gccggagcag atctggcgca gcatggaagg gatcgtgcga cagacgtgga
    11161 agcatgatcc gctgacggac atcgtcttca cctacacgat cacgacggcc atgacggagg
    11221 actatctgaa ggggatgtgc aaccgcgcgg cctcagcgat ggagctgctt gcggaccact
    11281 ataatatccc gtcgatcaat ttcggcgtgc gcgtggcaca gttgctgaaa gagaacaagt
    11341 tgattatgag cgcccgggag atcgaaaccg ctgtgccgaa agagtcgccg gatcgcgacc
    11401 ggctgatccg cgaacggatc gccgccgacc cccgtatgct cttcgcgaac gacggggtgc
    11461 atccgcggga cgaggggcac gagttgtaca aggccgcgat catcgccggc ctgacccaga
    11521 tgcgcgacct gccggcggtc gaccacaagg ctagtctggc gaatgtgttt gtacaggacc
    11581 attgggaggc ggctaaaatg gttccgatcc gggcatccat gctcaacggt aactggaagg
    11641 cactgccgga ggacgatccc aagcagaaaa gtttcgggaa gcgtatggga caaatctgga
    11701 tggccgatca gccgggcagc accttgcggt tcaagttcaa gggcaccgtg gccaaggtgt
    11761 acgatctgct cgggccggac ggtggtcaag tcacggtttc cgtggacggc cagaccggtg
    11821 ccaagcctgt gccgcgtttt gatagctatt gcacctacca ccggatcgcg acactcggag
    11881 tcttcaccgg gagcggtgcc gaggtgacac gcgaggcggt catcacgcta catcctgaac
    11941 agcccgaccg ctccagcatc tcgttccggc ttaaggaccc ggccaaagag ctggctgcgc
    12001 cgaattttca gggcacccgg atctggatga gtcaaatcat gctgatcggc gatctggtcg
    12061 agtaacgcaa tcgcactgcc agctttgcgc aatgtcaggt ggccgccgca gtgagttcat
    12121 ccgaacgctg ggcgatgttc tcgaggtggc gcacgatatt ttcgagtgcg cccacgacct
    12181 cgacgtaaac cagaccgcgt tccggtgtgc agaggccttt ctgcagacgt tccacatgcc
    12241 cttgtatgga ctggcgcgcc atagccttca ggtcgttgac cacgacctcc acggctttag
    12301 ccagaaggcg tccgtcctgc aggctttccc gtgtgagatt ggcgatgaca gacgctttgt
    12361 cgaacaaggc ttccagttcc tgcagcgccg tgtccgaaaa cttcgtgttt tccgaagtca
    12421 gcgaggccga gcgtttcgcg atgagcaggg cgaggtcgga gatccgttca gcgtcgttca
    12481 cgcagtgcat gagcacgggg atggcggccg cctgtttctc cgaaagatcc ttgcgggtga
    12541 gttgcaccag ataatccatg atgccggtct ggcaggcgtc gacctcatcc tcgatctcct
    12601 ggatgctgtt cacgtcaaca ggtttgccat ccttgtaacc gcgcaaagac cgggcggccg
    12661 cgttccaggc ggtttcggtc atatcaccca aggcgcgcat ggcgcagaag agcgcgagcg
    12721 ggggcgagct gagcaggttt ttctccagat gaacgacacg ggaggtcact ttcttacccg
    12781 gcatgatttt ttcgcagagc caggccaatg cgccaatgaa gggggtcatg aggatcacat
    12841 tcgtgacatt gaacagcgta tgggccatgg ccacgtgacg gccgatgttt tcacccagga
    12901 agacattgcc tgcggtcacg ctgttgacca ggtacaggaa acagggcacg ttgtggaaag
    12961 ggacgtaaaa gaggccgacc attacgcagg ttcccagcac attgaacagc gaatgggcta
    13021 cggcggtctg ccgggcggtg cggttggcgt tgattgaggc gagcacggcg gtgatggttg
    13081 tgccgatgtt gtctcccaag acgatgggca ccgcagtcca gatgttcaac aagccgctgt
    13141 tggccagcgc gatggctaga ccgattgtgg ccgaactgct ctgcaccagc atcgtcatca
    13201 gagtgccgat cccgatcgcg cccagcaccg cgccgatcgg catcgtttga cctgattggg
    13261 gtgcgcagtc gaagatatgg aaaaagcgga cgaaactcgg gaaggtgctg acgcttttga
    13321 gttcatccga catgagggtc atgccgtaaa aaaggaggcc gaagcctagg atggtacgtg
    13381 cggttccctg aagtgtcgct ttacggcaga gcagcaggcc ggtgacgccc agaatgatgg
    13441 caggaaaggc caggttgtcg aggttgaacg agaccatctg tccggtcacg gtggtgccga
    13501 tgttcgcgcc gaagaccacg ccgatggcct gtttgagcga gagcagcccc gcattgacga
    13561 accccacggt catgacggtc gtggcgctgg aggattggat cagggcggtg atgaaggtcc
    13621 ctgcgaagat cgccgccact cggttacggg tgacatacgc caatacggcc ttcatccggt
    13681 gccctgcaat ttgctgcaac ccgtcgctca tcagggtcat gccgaaaatg aagagcgcca
    13741 gtccgcccag cacgccgatc agcgtgcgga acaggcatat ggacatggcc tgaaggaaca
    13801 ccggccgcgt gaacaatccg cgtttcggat ccgccacctc ggcccggacc tcgtaggtcc
    13861 ccgtggcgtg ggggtcggtc ttgagggaga cctcggcgat cccgtcggga tcggtccggg
    13921 caaacgaggt cccgatacgg ccgtgttttc ccggttgttg ggtcagggta aagtgaaccc
    13981 ccgcatcggc gagcggtttg ccgtctggcg cgacaaggcg tacacgcaga ggtttcggca
    14041 agggatcacc ggcggagacc tcctgacgga cgttctctac cgcgacgccg caaacgaacc
    14101 gcagccgttt acggatttcg ggcgcctctt cgcagaccaa gtccagataa tggtctccga
    14161 acacggctcc gaacgtcacg tcgaatttgc acgatccgcc cgcatcggta agttgcgagg
    14221 gaggggagac gcgcacaccg cccgcagggt cgaccgggat cgcgttgatc cgcttgcctg
    14281 ccacagggtg ttgtgtgccc gcgccgccca ataggcttgc gacgggtttg ctcagcagtt
    14341 ccacatggat ggcgttcacc atattggtgc cgggcattcc aagcagggtt tccgacgaga
    14401 ataagaccag cttctccggt tccaagcctt ttggaacccg gtcgcatccg gtgaggaccg
    14461 ggagcgtaag ggcgagacac agcaggtagc gcacgaaggt ttgtgttttc ataagtgtga
    14521 aaaaggaaag gcctctctta ttcttgtgtt ctcccgttga gttgaaacag acaggaggac
    14581 tcacacgtta tcgcaggatc aggcatttca ggagagacgg atcttgccga ccagtcgcat
    14641 caggagcgct gtggcaacga ccgtgaccac cagcaggaaa ctcccggcga acatgcgtcc
    14701 gtagagcagg ttgccgacgc cgaagagcgc ggaccagacc gcgaggcaac cgatcatcat
    14761 acaaagcagg ccggtcggga catcccagcc ctctgcggat ttcggcagcg atgaggcctc
    14821 ttcgatatgg cgccagccgg ggccgcccgc gcggatccgg gtacagaacc gggccagcag
    14881 atcctggttt tcgggagggg tcagataggt cacggtgagc cagcctatgg tggtgatcag
    14941 gatgccgatc accagtttcc aggcgctgta atccatgacg gcaaagagtc cggcacgttc
    15001 catggccgca tccaatccaa gcggcgcggc accccattga aagaaacagg ccaccgcgaa
    15061 cgaaatcacc atggcggaga tctcgctcca ggcattgatg cgccaccaga accagcgcag
    15121 gatatagagc agcccggtcc cggcaccgat ttgtaacaga atgtcgaagg agtcttttgc
    15181 attgcgcatc cagagcgcga cgagtccgga gagcacgagg agtgcgagca tggtgacacg
    15241 gcccatggct accgcctgcc gcggcgtact gttggggttg atgaaccggg agtagaagtc
    15301 ttgcaccaca tacgaggaac cccagttcag gtgtgtggcg atggtggaca tgtaggcggc
    15361 gatcagcgag gccacgatca ggcccagcca ccctttgggc atggtggaga tcatccccgg
    15421 ataggcgatg tcgtggcgca ggaactgatc gtcgacttta gggaacgccc gggcgagact
    15481 gcccacccct gcggcatccg ccttggcctg ccgcacttgt tcccgctcgg gttccggcat
    15541 ctgcattttc gcggtgtcat attgtccgac ctgtgccgga tggtctgcga gccatcgcgt
    15601 tgcggccgcc tgttccgccg gaggcgtgag cgggaagtgc acaagcgaga cgagagccac
    15661 gatgatccaa ggccaggggc gtaccgcata gtgcaggaaa ttgaacaaca gggtcgctcc
    15721 caccgcgttt ttctcatcct tggccgagag catacgctgc gcgatgtatc cgccgccgcc
    15781 gggctccgcg ccgggatacc agacattcca ccactgcacc gcgatcggca gaatcagcac
    15841 ggtcatcagg gccgaaagcc cgccctctcc ggacgggaac atcgacatgt gcgtctgcac
    15901 ctccggttgg gagaagagct gcttcagcga agtgatgcct cctacgtcgg cggatttgac
    15961 ggcgtaaact gcggcaaaaa tcgcgccgac catggcgatc gaatactggt agaaatcggc
    16021 ccagatcgag ccggtgagtc cgcctagggt cgcatagatt cccacgccgg ccgaggcata
    16081 gagcagggtg gcgaccggtg agaggccgaa catcacctgt ccgatcttga tggctgccag
    16141 cgagaccgtg gccatgatca tgatgttgaa gaaaacaccc agatacaggg cgcggaaccc
    16201 gcgcagcagg gctgcgggtc tgccgctgta ccggatctcg taaaacccga gatcggtgtc
    16261 cagcgcggaa cggcgccaga gtttagcgta gacgaagacc gtcagcatgc cggtcagcag
    16321 aaaggcccac caggcccagt tgcccgcgac accctgtgtc cgcacgatgt cggtcacgag
    16381 attcggcgtg tcgcacgaga aagtacaggc caccatggag acgcccagca gccaccaagg
    16441 catggaacgc ccggagagga aaaagtcggc tgcgctccgg ctcgaacgac gcgctgcggc
    16501 agagccgatg gcgatcacag aaaagaggaa gccgaagatg atgacggcgt ccagagtcga
    16561 gattttcaac atgttttttt cctttcacac tccggttacc ccggaaagca tacctcgtct
    16621 tcccgacggt tcggtcatga cgcatccggc ggaatcaggt ccgcgacttt cactcgaacc
    16681 gcctcgcgct cgcgttccag cgtccgcaac gtggggtcat ccgccggaac gacggcggga
    16741 tgggtttgat tccacttcgt aaaggctgct gaggttcggg tcacctcgct ttggaggcga
    16801 acctgctcgt acttgagcgc gcgcagggcg tccagaactt tgaccttgcg tgcgccggtg
    16861 catgtctgcg cctctgcttc aagccggtcg gcttgggcaa ggctctgctg ataggcgcgt
    16921 tgcgcgtcac gcgcggcaag gaagtgggga ttggccgcat gcgccttttc cgccagcgtc
    16981 gctttatacg cggcgatttt ggcggtgagc aggaaaaagg agtgtagatt cccgacaatc
    17041 tcgggatcca cggtttcggg gtctccctca taggtggcgg tttccgtgca gtcgagcagg
    17101 cagggttcgt cccacgcatt gttgcggcgc acccggctca acagcatcgg gttcttggac
    17161 gaggctttca catcggaata acggaacagc gtgccgccgg gcatggtgcc gagacgcttg
    17221 ccctccggcg aatagtacga cacttcgcgt ttcgtgaagc cccagccgct tttcggcccc
    17281 ataaacacca cgcgtgtgac acggttctgc ggggcggcgg tttgcgtcaa ggttttgtca
    17341 gcggaagcgg tctgcccgct gcggttggat cgccgctgtg cgggctgccc agtcaaccgt
    17401 tgcgggttcc gggcggaacg tttcacctct gccgttccgg acagggttat tccaatcagg
    17461 cagaggcaga accagacgtt gttcggaaga agtcgcatag caggccgatc aaacactgtt
    17521 aaagagtgaa tcatattcgg ccccatgttc caagcaggtt tttcgtcgga tgaatcgatt
    17581 acgggaaaac agttgccgcg gttgatggat acgcgtatgg taatcccatg ttgcggcaaa
    17641 acaagaggcc gtgcccggag acgcgttttg cgcgcggggt acgggcattg cggaaactca
    17701 ccccgggact gaccgaccgg gaacgcgcgg ctttgtggct ggtgcttgcc ctgttcgtct
    17761 tgggagcact tgtgcgctgg ctccgcatgc aagacggttg agggaggcgt gtgaaccctg
    17821 ccgtgtcacg atgcgccccc tgatgaggca gggggcgcgt atcggactaa ggttccggcg
    17881 gcgtgttcac cagcggcagg actccgatat tggggtgctg ttcttcgatt acccaacgga
    17941 agaaggcttg agccggatcc cagtacggga aactgtagat atacgggttg ggcagcagaa
    18001 tccggcactg atggttcgtg tcgaacgaat ccggcatcag cctgtattgt ccggcgatcc
    18061 gccaatcggg gattgtcagc gacggcgtca tttccagttt catgaccgcc tggctcaaca
    18121 gacaggtaca gttctgcccg ttgacggcca gcgaggccgt aacataataa ttggtttcgg
    18181 catcacgcgt gaaacggaca atactgccgt ccacacggtt ggttacggtc ggatccgcat
    18241 tgagccaata cagttcggtg agggacatca acctcccgtt gccgaagaaa taactcgatg
    18301 caagcggagt gttattatcg ccgaacccga gcagccagtt cagcatatcg tcgctgattt
    18361 cgagatcagc gatatcctga cgcagggcga aatgagcttc cacattcagg ttggcagaga
    18421 cattggtgag cggtaacaga tagttttgca agccttctcc gggcggaagt tgttccacct
    18481 gattgacctt gacggaatag agacggtacc agttgtcagc ctgatacaga atgttggtgt
    18541 tcgcgccgtt ccgcgtgcgg aaggtatggc actgcgtgcg caaaccgttt tgcgtaccgt
    18601 tcaacgcatt gagtgtggac acgatccagt ggttcgagac gcccagttcg gggaaggcga
    18661 cagactgacc ggggttcagc ccgccggggg tccaatagag ggggaaggtc cagtccgcgc
    18721 gtgtgacacc ggtgccgttg gtgacattga gcgaaccgcc atgggtctcc ggtccgacat
    18781 acacgaaccg cctttccgga tcttcggccg cccagagttc gccatcgaat tccggtccgg
    18841 agccctggct cgggtcatcg tatgcgatga catgctcgta catgcccatc ggtcgtttca
    18901 ggcgcaaccc gcccgcgtag atacaaggga tgtaccagta aaagccgggc tccccgtaat
    18961 cgagcttcgg taacggcacc ggtgttctat tgggatccgg aaatttctcg ccgatcacga
    19021 agaacgcgta gccattggtc accgccgttt gttcaggcag tccctcgggc agggtgatgg
    19081 tatgcatatc cctgtcattc atgaccaggt cgatactcca gcccgcgaga tcgacaccga
    19141 cgggcacggc gatttcgatg tacggattgt cataaaatcc atattggggc acaccgttct
    19201 cctcgacatg ttccgccgcg tttacctcgt tgatccacac cgttttgagg ggtacgccgt
    19261 acacgatgta atagggggac cacccctgcg aagcgtaaac cgcgttgaaa tccactggga
    19321 agtaccaagg aggattctca aacgtttttt gttccgcagt caagggtatc cctcccagat
    19381 aggtggcgcg cacacggtac tgcacgatgg tgtcgctgtc cagcatggga aggtcctgag
    19441 aggggagggt ctgataggtt gtcgagttgc ccatttgctc catcggcaac gtcaccacct
    19501 gcgccgcagg ccagttctcg acgccccaga cattggtgcc gatatagtaa tcgacataca
    19561 tctggatgtt cgaaggcgag agccggatgt tcaccaactc cgcctgaatg cccacgtggt
    19621 cggagttgat aggctggtgg cggggcgggt catcatcata cgaatcgccg ttgcggcaga
    19681 gcacctgcac attctcgatg tcgaaacccg gaaagactgg ttcggtcacc gcgatatcct
    19741 caatacaggc acgctggccg cccagcgggg aaatgaattt gatcgcatcg taatcgcgtc
    19801 cgtcaaccgg ttttacggtg accgtttcat aaaaccggtt cgtgatctgg gtgaaggcgg
    19861 cgatctcgaa ccagaggttt gtgggcgcat tccatccgtt ggtggtcgcg taaatgtaaa
    19921 ggctcgacgg aaaagtattg gtgtaggcgc gtgtcttgaa ggagagttcc cccaaaccgt
    19981 acggcagaac cggggattgc acgaacggca cgttctgatc ctgaatgggc tccgcatcct
    20041 gcgtggtgct gttgttcagc aaacaggctt cctcgccatc caacatgatc cgctgtttgt
    20101 cggtttgggt cacctttaca ttatacgcgc gccatgaggt gtccttaacg acaggctctt
    20161 cccaagcccg gacattgtcg atttcgaccc aggcgttgga acacccgccg cttcggtcgt
    20221 tgatcacccg caggaatccc ggattggtaa tgcccacgta tcccgagatg tggtcccact
    20281 cggacatcac gttgctgatg gcgtaggtgt gcacggggat ccagatgtcg ttctcgcgct
    20341 ccgtcgcgta ctgcacggtg agctgtgcgg gagcgcgcag cacacggtaa tcgaactcca
    20401 gcatccccat gccgttttcg aggaggatcg agcggaccgc ctgatccgca ttcggatcgg
    20461 cgcgcgtgtg tttgagctgt atcacatggc cgttcgccgc attgttggag acggcccaag
    20521 cctcggtggc gagccaggcg tcgtcattgc cgctaccgaa ctgttcaccg cgccaggagg
    20581 ttatcttcaa ctcatccacc acgacatcgg ctttcgatcc cgccactccc atgacctgca
    20641 acaaaaggaa ctgtgtcttc caatccttaa ccgttacggc attggtcttg tatccgtacc
    20701 ccgaaacggt gatctcctgg aaaagcttcc atgcggtcgt gcccggcgcg gcgggctcgg
    20761 cggttgagtc ctgcgcggag ctctggaggt aaaccccgag tttctgggtg gggatgaccg
    20821 cgtggatccc cttcggcgac ctatctccat caagaacaaa acggcccgag ggggcactcc
    20881 agttgttttt atccgtgttg aacgcgttcg tatccgacag gacggttatg gcgctgccgg
    20941 tgagggtcgc tccggttgtg gtcggttgga taatgaaatt cgagaaacct gcaccgcaat
    21001 cctcggagag cacgccgtag gtgccgtagg tgaggcggga atcggagttg tcggtatgat
    21061 cgatccgtaa tacagcgctt gatgacggat aatagtaaca gcggatcagc gtactgttgg
    21121 gttggttgaa tatacggatt tccatagcgt gttcgaatcc cactatatcc cccgcgttac
    21181 cggtgtcata cgtgcctgag gttgcaagca gggtgggcgt gccgttgatc cagcggtaca
    21241 attgatgtgt cacccgcttg tcgcgtgaca gattatcggt gtcgggaact tgagtgatgc
    21301 ggtattcgta aaaatggtcg gcatcctgat aataggcgat cacggagagc gacggggcgt
    21361 ccggtgagtg tcccgtgtag gaacgggccg tcactttggc cgaatagttc gaactgatga
    21421 acgcggtacg gtcgtacgcg acgtcgattt tgtcggcagt ctgcgccaga cggtatttga
    21481 aggcgaaatc ccggatgccg tcggtccggg tttgcaccgt attgtggacg taccccaagc
    21541 cgagggtaca gccaccccct tggagacgca gcgcctgatt gcggaagttg acataccctg
    21601 ccggcttgtt gttctcgtcc gccagcgttc gttcgacact gaaggccgcg tgaccggcca
    21661 tccagttggc ctggggggtg ccgaacggct cgcggacaaa gacgttcgtg gtggggacgg
    21721 gcagatggaa gtattgggaa taggtgtcct ccacactgac tggccacgcc tcaaaggtgt
    21781 tgagaaaact cgctttgggg gtttgcccgt tactctccga aaacttgtcg gagagggccg
    21841 gccaattgtt gaaattctgg tactcggcac ggacgagcat gtatttcaag gtttccgtgt
    21901 cgaaagtcag catcacatac gcgccccgat ccgccgtgac cgtcttggtg agtggcgttt
    21961 cggtttcctc gcagaccccg ccgtaaggca ggcggttgat ggtgccgggg agggtttccg
    22021 cccagtggac aaccgaggtc gaataggcga cctcgaccgg caggtagcgg cgttcccctt
    22081 ttaaacgcca ggagacattg gttgtgcccg cgtcgctgag cggcagcatg ccctgccaag
    22141 tgttgtccgc cacaagttcc atggtgaccg gttccggata ctcgtccgcg actacggaga
    22201 tcgaaccata gggcgagacg aaagggcgga ttcggatgga gaacggttgg gaacggcttc
    22261 cgagcaggcg gggcgagaaa cctgcggccg cggcttctga aggatagaca tggccttcac
    22321 ccgtgtaatc gggcgactgg taactgtagc ccgcatacga gcactcgaaa tagtactcca
    22381 agtcaccggt ccgcgggtgg ggcgggatca cgccttcata cccctcgtta ttcccctgga
    22441 tgccctgaaa gaaattggtg agggtgacac tcgtccagtc attgctggcc tgatccagat
    22501 aacgccagcg gtaatagact ttgtttgtgc ggctggtcca gtcggtgggc gtgttcatgt
    22561 ccacattgtc cgtgaaacag cggaccgtca tgttcgtgcc gactgagggg tatcccggct
    22621 gaaaagggca ggcggttttc cggaccacga catccgatgg cggttgcgag acccggatgt
    22681 tgtcgatgac ggtgaagaag gaatcgagac catctccagg cgcatccgtg taaacacccc
    22741 cgtaattttc cgcttcagga gggggaagcg cggagccgcc cgaaaccccg ccgctgcgct
    22801 gccagcgaag tttggcggga ccccggtagt tcagggagcg tacataacgg taaaactctc
    22861 cggaattcga gtaatccaaa acaacatcat cgccaaattg tacccagtta taggtgaggt
    22921 cttgggtttc cgcgtcaagc aggatgttgg tgggccagcc gctggtttgg tcagtcatgt
    22981 tggtggtcag ccagatcgtg atgcgggtgg gccgcccggc ttggttgttc accgcttcaa
    23041 aataaacggt gccgatccct tcttggaaaa caggcgagat gatcgccgca tccaacgtgt
    23101 tttgcaggtt ggcggcgaaa ttggtggcgg tgttaatgtt gaaagaaggc aatgtggggg
    23161 ggacgatcag cccggatcct tgtccgcggg cagaaccagt gcgcgtgtta aaaatattcc
    23221 atccagcgga atgaaaatgg tttgtgcctg agcgtaaggc tcggcgagta gcgagccctg
    23281 taattaccgg cccctcatgc gtccaaagcg aggtgtccgc ataatcatca aacagcagga
    23341 tacgcgctac cggcagcagg ttggtcacgc cggacgcgac acctgtgcag gtcaagaaaa
    23401 ccgccagcgc ggcgcacaaa tcgttactca ctcgtttcat tccgatgctc cacacacgtc
    23461 tgtccccgtc cgcaaaaaat ggccgctccc aacgcgctgg ttcttgctca ccgcatccgg
    23521 tccctcaact cttcggtgat atcctcgcca ttgcagagtt gccttgtcag ttcgatgcag
    23581 tcatccttgt tccccccttc gggttcgatc aacgcatcga catggccatc ggcataggcc
    23641 acatgcgcca cgtaccgttt gccgactttg tggttgaatc cgatcatctc cggtgacccg
    23701 ggattcgcat agtttttgat ctccgcctgc agcacgctgt cggcaccctc ccccgaagag
    23761 acgatcgacc gcgctggaag ttccgcgaag agcaaccggc tggccgcatt gccgcttgag
    23821 gagagacttt ccagccagcg ccggacatcg gcggttttcg ggggggcttt atgctcgtaa
    23881 ccgaaatagg cgttcattac ataagagcgg tggattttct cgagaccggt tcgtgcggcg
    23941 gctttcttgt gcgtgtcgca aaggaaagtg gagacgtctt tgcccacaaa cgtccataaa
    24001 ctgccgttcg tgagcgagta gtacgcattg ttcccgtgaa aacacgagat ctccatgttt
    24061 tggatatctt tgttcggcca acttccggat ccgacccagc ctacccagcc gcggtgctcg
    24121 tggtatttcg ggacgtaacg gttgccatcc cggctggggt aacgcacctg gaacgaccct
    24181 gcccaaggca tgtgtttggt ctccacggca taactcaggg aagcctgcgc gaggttacgc
    24241 aggttggtct tgcaccgaat cgcctgcccc atggaccgtg cccggcccac ctgtgtcgtc
    24301 agggcagcgg ccagaatgcc cagaatggaa atcaccacca gcagctcgat gagcgtgaat
    24361 ccggtttttc gcacaaaaag cataaccctc ctgtgtcttt ttttatcagt ttgatattgt
    24421 cgctgttatc gcctcaagtg gcaaggccta ttctcactct tttttgactt gcgaatcatc
    24481 ggaaaatacg gtgaaataaa tcgcaatgtt ttcgtaaagc gggatttaag aaggataaaa
    24541 gcgaaatgcg ttggtcgcgt gcgttgctca ataccctgcg ggatgatccg caggaggctg
    24601 aaatcaacag tcataaactt atggtccgcg ccggcctgat ccggaaggtt gcgggagggt
    24661 tgtatacgtt tttgcctctc ggactgcggg cattgcacaa ggtgacccgg atcattcggg
    24721 aagagatgga tcgggccggc gctcaggaga tcctgatgcc cgcgctgcag ccggccgagc
    24781 tttgggagcg ttcgggaagg ctcgccgcca tgggaccggg gatgttccgt ctgaaagacc
    24841 gtgcggagcg tctgatgacg cttggcccca cgcatgagga ggtggtcacc agtctgttgg
    24901 cgggtgaagt cagttcctac cgtcaactgc cgtgcacggt ttatcaggtc cagaccaaat
    24961 tccgtgatga gatccggccg cgcttcggtc tgatgcgcgc caaggagttc atcatgaagg
    25021 atgcgtacag tttcgacacc tccatggagg cggcggaccg ttcttatcag gcgatgtacg
    25081 atgcgtatac ccggatattc cagcgttgcg gcctgcgggt caagccggtg gaggcggata
    25141 cgggggatat tggcggaagc tggtcgcatg agttcatggt tctggccgac tcgggggagg
    25201 acggcattgt ggattgcgcc gcctgcggat atgccgccaa tcaggagcgg gctgaaagga
    25261 tgggcaagcc tgcggcggaa gcggcctgtc cccccttgtc tgaggtgccc acccccggag
    25321 tgcgcacgat cgaggacctg accgcctttt ttgactgtgc gccggaccgg ttcatcaaaa
    25381 cgttgatcta tctggcggac ggcaagcccg tcgccgcatt ggtcccgggt gaccgcgatg
    25441 tgaatgagca taagttgaaa cggctgctca acgccagcaa gatcgaactg gcggatgagg
    25501 aaacgatctt ccgtgtcacc ggtgcgccgg tcggattcgc cgggccggcg gggctttcga
    25561 ttccggtgta tgcggatcag tcgctggagg gtgcggaaga ccgtgtcacc ggtgcgaacc
    25621 gcgccgacac gcattaccgg caggtttccc tcgcacggga cgcgcgtgtg accgcatatg
    25681 gggacctgac ggttgtcggt tccggggatc tctgcccgcg gtgcggcaaa cccctgcaac
    25741 tccggcgggg tattgaggtc ggccacgtct tcaaactcgg gaccaagtat tccgagtcgt
    25801 tcgatgccgt ctatctcaac gaaaaaggcc agagcgagca gatggtcatg gggtgttatg
    25861 gaatcggcgt gacccggacg ctgcaggcgg tcattgaaca gagccatgat gccgacggca
    25921 ttatctggcc tgtatccgtg gcgccttttg cggtaagtct gctgttgctc gatccccgtg
    25981 acgagcgggt ttacgatgtg gtcgctcaac tggaggcgga actggaggct tgcggcattg
    26041 atgttttcgt ggatgaccgg gaggagcgcc cgggggttaa attcaaggat gccgatctaa
    26101 tcggttgccc ggtgcgcgtg gtggcgggcg cgaaaggcct cgcgaaaggc ggggtggaaa
    26161 tcaagttgcg cacaggtaag gagagcgaga tcgttccggt cgagcaggct gcggcggcga
    26221 tcgccgaccg cgtaaaggcg ttgacatccg cactcagtgc gaaagcggac gagcctgccg
    26281 cctgcgaagc ggggaatggg tgagggtaga atggaacggt tacgtccagg gggaagggga
    26341 gggatgccat gaagccgtca aagtatgata aagcgccgga aagggtggcg ttgaaaaaac
    26401 gcgatctcgt caaccggatc gccaaagata ccggactgac gcaactgcag gttctggata
    26461 tcgtccagag aacgctggat ggtatcgtcg acgcgctgga agccggtcag catgttgagt
    26521 tccgcgagtt cggcgtgttt tcggtggtta aacggaaagc gcggatcggg cgtaatccga
    26581 atcaacccgc cgacgtggtc acgattccgg tccggtatgc cgtgaaattc aaacccggca
    26641 aacgcatgcg agacatgctt gcggataaag tgtagtgggc tgccgcacgc ttttaggttt
    26701 cccgtcatga atgtacccgc ttcgttttcc tttgatccgc cgcgcggcat gcgcgatttt
    26761 tatccggaag acatgttttg gcgcaaccgg atctttgacg cctggcgcca ggcggcgctt
    26821 gcgttcgggt ttcagcccta tgacgcgtgt gtggtggaaa gcctcgatct gctgcagcgg
    26881 aaaagcgggg aggaggtctc ggagcagatt taccattttg aggacaagag cggccgaaga
    26941 ctggccttgc gtccggagat gactcccacc cttgcccgcc tgattgccgc acggcagggg
    27001 caactcgtgt tcccggtcaa gtggttcacg gtggcccaat gtttccggta tgaacggatg
    27061 acccgcggtc ggaaacgtga acattaccag tggaatctgg atgtcgtcgg ggaaggtcag
    27121 gtctcggctg aggttgaggt gttggccgcg gccgcagagg ggttgcgccg catgggactg
    27181 ccggagcagg cttaccggat tcatgtcagc aaccgtgccc tgctcgcgga gttgctggca
    27241 gtatccggga ttgcgcctga acaccatgcc gcagtcttcc tcgcgctgga taagcgcggc
    27301 aaaatttcgg atgatgagat cgccgggttg cttcgggccg aagggttgaa agaccctgcg
    27361 gtggaacaga ccttcgcttt gctcggcatt caaacgctcg atcaggcagc ggagcgggtc
    27421 ggtcccgatt cggccgcggt cgccgcgttg cgtgaattgt tcgcgctggc cggggtctac
    27481 ggcatcgcgg atcgtttgat gttcgatatt gccgtgatcc gcgggctcgc ctattacacg
    27541 gggatcgtgt tcgaggcgtt cgattcggcg cgcaaattcc gtgcgatctt tggcggcgga
    27601 cggtatgaca atctgctgac taccatcggg ggtgcgccca cgcccgccgt gggattgggt
    27661 ttcggggatg tggtgatcgc ggaggtgctc aaggacctac tcagcgagac ggcggcaacg
    27721 gaaaagaatg gggtgatcat cggatacatg ttccctgaac agcgtgaatc cgcgatgcgt
    27781 ttggcccgga gcctacgcga ggcgggtgag agggtggacc tccacctcgc acgccagaaa
    27841 ccgaaaacgt ttttcgccaa agccggtgcg ggctctgcgg cggacgcgat tttcatcggt
    27901 cccgatgatg tggcgcgcgg cacagtccgg cgcaagcatc tcgctacgcg cgaggaaaca
    27961 gtcattccgc tgccggaggc gtgaacggca tgcgcccggc ggtgtccacg ggaaccgacc
    28021 cgaccgggtt gggttgccgg gacggaagcg gtttcgagac gcgccgcgca ggtcacgcga
    28081 aaagcgagcg gacaaaatca ccgttgcgtt tgccgtcggc cacaccgtcc ttggaggggc
    28141 ttgaggtctc cagcacgatc catcctgaat accggatctc ccggatcgca gccgtgaccg
    28201 cctcaaaata ggggcggtcc tcatccagat accgcgggcc gtttttgtag tggatctgcg
    28261 taatacgccc ttttagaagc cggatttcgg cgggcgagtc gtatttcatg ctttttccgg
    28321 tattgtacac atcataataa aggctgaccg cctcatgccc gatcgtgtcc agcattcgaa
    28381 ggttctgctc ggcgctcaac aggttttcga tggagagtgt gactccggcg tcgtgcgccc
    28441 gcggcgccgc atcccggatt cgtcggcaag cggaggcgaa ttccggctcc ttgatttttc
    28501 gatcggcttg gaggtcgcct ttgccgaaaa acgccaccag gatattcggc acgcccagat
    28561 cctgcgccgc ctcaatactc tgccggagcc aggcgggggc acgaggatcc gtggcgaggg
    28621 gatacgagtt caacagtccc aacatgaggg aacaaatcgg caatccggtc tcgcgaatct
    28681 gcgttttgag acgggcgcgg gtatccgctt tgtagatatc cagttcatcc gccgccccgc
    28741 ccgcccagag ttgcacacca tccagtccgg cctgtcgggc ttgatcgaga cggagcgtgc
    28801 aggaaccgac tcggattttc ggtttatccg catgcgcacc cggggacggg atcgcggtga
    28861 gcgacagggt ggcggcggaa tgccggagga acgatctgcg ggtaaacttc atgacgtctc
    28921 ctttccttgc aacgcacgtt aacggaggac aaaccccgtc ttcggtgaga acgtgatccg
    28981 gtaacggcag gtgggaacct cgcggctttc gacgcggatc agagccagcg ggttgcggaa
    29041 tccgatctgc agcaactcct ccagcgtgaa caggttgagc cggtggagca cgttcataca
    29101 tgcgatcagc gtggcggaag aaccgaccag gcattgtttc tccggattat gcaaccgtcc
    29161 gtccggctcc agtacggcct gattccccag cacattgtag gtcccgggcg gcaatcccgc
    29221 tgcaggcgag gcgtccgtag tggcgatcag acggcgcgcg cccgcggcac gggcaaacac
    29281 tttcagcacg tgggcgggga gatgatgccc gtccggaatg aacaggatct gcaagcgctc
    29341 ctcggccaag ccggtcaaca gcggattgtc gtgccgatga gcctgattcg ggcagccgtt
    29401 ccccagatgg gtcagggccg aggcccccgc atcaacgagc cgcgcaaggg ctgcggcatc
    29461 cgcgagttgg tgaccgcagg agacaaggat gccccgcgcc acggcatgtc gcgtgaggac
    29521 ttcggcaccg ggaatctccg ccgcgatcgt cagcaggcgg atccggtctc cggcccactg
    29581 gcagagccgg tcgaaatacg cgcaatccgg gggacggacc gctgcggggt catgtgcccc
    29641 caccgctccc ggctcgctcg aaatgaaggg gccttcgagg tggaatccgg gaatgtgacg
    29701 cgccagcacc gcatcttttt ttaaaacgga cgccatccgc tcaagattgc ggcgggtctg
    29761 ctccggggtg ccggtgatta cggtagggag aaaagcagcg gtgccgcgtt ccagtagcgt
    29821 atggcaggcg agtttaaagc tctcttccgt gagatcggga ccgctgaaat ccacacccac
    29881 aaacccgttg acctgaagat ccacaaatcc ggggatatcc agtttgacat gcatgctgag
    29941 agaataaggg attcgatggg gcggggcaag gatggaatac cggaatccgt ctggcgtttt
    30001 ccggggcagg tgtgaggttg cgcgggactg cgcagatgtg ttatagtttc ggaaatcttc
    30061 gaattatgag aagggcacgg catggattgg cgagcggagt tgagcgggat tttgggcggg
    30121 agagcccgga ccacgcgtgc ggaagaggaa aacgctgttt ttgaggcgtt tttggatacg
    30181 gtggccattc cggcgttgtc cgagatcgcg gaagagttgg tccagaagca cgaacgtgat
    30241 gcgcaagtac ggcgggcgcc cgcttcggcg acgttgagtg tgcgcgtcgg tgagatggag
    30301 gaaatcacat tccgggtgat gaagcagttt gtccagaacg ggattctccc gcgggcggag
    30361 gtgcgcttga atcgcggaca gcggctggtc aaatacgaga gcatgttcag agcggatcct
    30421 caaacgtatc cgatcacgga gataactcag gatgaagtga tcgcctgctt cataaaatat
    30481 taccgcatgg tgatggatcg aaacccaacc gcagccgagt gagtgccgta aagaccggcg
    30541 tgtgcgacgc cgtgtttcgg aaaagttgat gtcgtgaagg attccgtgcg tatgatgaaa
    30601 atcccgttgc cgaaaataat ccagggtggt atgggtgtcg gcgtttccca ttggcgtttg
    30661 gcccaggccg tatccaaact cgggcagctt ggtgtggttt ccggaaccgg gctcgatcag
    30721 gttctcgccc ggcgtttgca ggatggggat ctggggggtg acgtgcgccg ggcgctttcg
    30781 catttccctt tcccccagat ggcgcagcgg gtgttggacg cattgtttgt gcccggcggg
    30841 aggccggcgg accagccgta tcaggctgcg accaaacata cgatcaaaag caaccggtgg
    30901 gtggacgagt tgtgcgtcgt ttccaatttt gtggaggtct ttctcgcgcg tgaaggccac
    30961 tccaatccgg tcggcatcaa ctatctcgaa aaaatccagt ttccccacct gccctccgtc
    31021 tacggcgcaa tgctcgccgg tgcggcggtc atgatcgtcg gcgccggcat ccccgtggag
    31081 tttccgggcg tgttggatgc gctgtcgcgc catgaagccg cgacctatac cgtccgcgtc
    31141 cacggcgcaa cgcctgagac cgactgccag cgtgtctttg atccggccct cttttttgag
    31201 gatggctgta cgccgccccg tctgctgcgt ccggatttcc tgccgatcat ctcttcggac
    31261 tcgcttgcca ccatgttgtt gcggcgcgcc agcggtagcg tagagggttt tgttgttgag
    31321 ggaccgacgg ccggcggcca caacgcgccg ccacgcggtc cgctacagct gacccccgac
    31381 ggccagccga tctacggtcc gcgcgatgtg gtcaaattgg aatccatgcg taaaatcggc
    31441 atgccgttct ggctgggcgg tggctacgga tcgcccgaag ccctgcgcac ggcactcgca
    31501 gagggtgccg tcggtgtgca ggtcggcacg cctttcgcgc tctgcgaaga gtcggggctg
    31561 gagccgaaaa tgcgccacgc tctgatccat ctggcgctgg ctggcgacgg caaggtcttt
    31621 accgacccgc tcgcctcgcc aaccggcttc cccttcaagg tcgcacggct cgaagggacg
    31681 ctttccgagc ccgaggttta tacggcgcgc cgccgggtgt gcgatctcgg ttatctgcgc
    31741 gaggcctatg tccaagcgga tggcgtcatc gggtaccgct gtcccgcgga accggttgag
    31801 gcctacacgg ccaagggcgg cagtgcagag gatactgtgg gccggaagtg catctgcaac
    31861 ggcctgttcg gtaatatcga gctgggacag gttcgggcgg acggcacgcg tgagccgggg
    31921 attgtcacgc tgggtgacga ttatctgcgg atcggccgtt tctgcacgct tgaacagccg
    31981 gactattcgg cgtcccacgt cgtgcgtgtt ctgctgggtt gaggcggcga gtctgccata
    32041 ccgcgctgcg ctggaaaacg catcggtccg aatcagagcg taaagcccgc aagcagcgtt
    32101 tgccgccagt cgcgcatgag cgggtgcgcg gaggcgtctc cccgcgcaag ggactggacc
    32161 aggctttccg cttcgttgcg cgaggcgatc catgccggat cgatcccctc tttttcgcag
    32221 gcgatccgga gatgggcgag caggcggtcg gttggctctt tgagtgcgcg cgcggtcgcg
    32281 ggattccggt cagaggggtc agggcatgcg ctctccggca acgcctttcc ccgttccacg
    32341 gccgccagga tttgtccgac aagtttggcg ggcatgcgtg aaggaaaacc ggggaccgcc
    32401 atgagcgccc ggacatctgc cggacctcgc aacgcgatcg tcaccatcac ggggtcgggc
    32461 agcacgtgat tccggggcca gtcacggcgg cgggcttccc gttcgcgcca ccccgccacc
    32521 tcgcgcagga gtgcgaggtg gcgccggttc aggcgggcgg cgcctttcac gcgccgaaac
    32581 ctttcctcag gcggagactc ctcgtaatgt gccggatcgt ccagccgcgc gagttcttgg
    32641 tccagccagt tccgggaccg ctttccggcg cagcgcgcca gcagggcttc gcgaagcgga
    32701 agcaggtgaa caacatcctc ggcggcatat cggatctggt tctcgctgag cggtcgccgt
    32761 aaccaattgg aacgggtctc gcctttggcg atcgtcaccc cgaggacctc ctgcaacagg
    32821 gcttggagcg agagcgttgc gctgagtccg gcgaacccgg ccgcaaggcg cgtgtcgaat
    32881 acggagcgcg gtacggcacc ggtcgcgcgc gctaggatcg tgaggtccag aggtgcgtca
    32941 tgcagcactt tggttaccga cggcgaggcg atcaacgggg cgagcgcaga gagatcctta
    33001 aggcataccg catccaccag ccagcaccca tcgggcgtgg cgagttggac gagtccgaga
    33061 accggataga aagtgcgttc ccagaagaat tccgtgtcca gcgcgacggc ttcggcgcgc
    33121 agcagacggg tattcaggtc ggcgaggtct gcggccgtag tgatcagggt tggtgtcaac
    33181 ggcaccccct taccgctttg gcaggtggat gcaggcgtca tgaaatgcgt gcgccgcctc
    33241 catccacgcc tccgagaggg tcggatggca gtggatcgta ttgccgatct catggaccgt
    33301 cagttcgtta cggatcgcca gcgcggcctc ggcgatcagt tcggtggcgt ggcatccgac
    33361 cgcctgcgcg ccgagcacct ggccggtgac cggatcgcag acccacttga cgaagccgtc
    33421 ggtctctcct gcggtcatgg ctttgccgag cgccgagaag gggaaaaccg cagtcttgac
    33481 cgcaatcccg cgctgccggg ccatgttttc ggtgaggccg gcgagaccga tctcgggcga
    33541 ggtgaagata caggccggac agatccgctc aaccttggat ttcaggcccg cggcggcttc
    33601 ggcggctgtc acaccctgct gggtcgccgc gtgtgcgagt tggatcgaac cggttaccag
    33661 atcaccaacc gcaaaaacag atgcaacatt cgtgcgcccc tgcgcattca cggggattgt
    33721 gccgcgctca tcggtcttaa tgcccgcgcg ggcaaggtcg aggagggcgg tgttgggctt
    33781 gcgtcccacg gctacgagca gcacgtcacc ctcgacgcgc tgatccttga aggttccggt
    33841 gaccttgttt tttttcacgg cgatgtcggt caacggcgta ccggtgagga gtgtgatgcc
    33901 gagcgtttcc atacgtttgc gcaacacgcg gcgcacgtcg cggtcgagca cggtgacgat
    33961 gtcctcgagc atttcgacga ccgtgacgtc cgcgccgagc gcggcggcca tacaggcgaa
    34021 ttcgcagccg atcacgccgc cgccgaggat gagcagcttc ttggggagtt cggtgagatc
    34081 gaggaaggca cggctgtcca tcacgcgctt gtccttgggc aggaaagcgg gcatggccga
    34141 atcgctgccc gccgcgatga tgatcgagac ggcgctgagc cagcgcgtgg tgccgtcggg
    34201 ctggaggacg gagaggcggt tcgggccttc gaagcgggct gtgccgttga tcagctcaac
    34261 gccattcgat tggagcagca tccgcacgcc gccgctgagc ttgtcgacga tttcggcctt
    34321 gcgggccgtc atcgcggcat agtcaaaggt cacgcctttg gcagtgacgc caaacgttgc
    34381 ggattgggtg gcatgatggt aacgctccgc agaggcgatc agggttttgg tggggatgca
    34441 gccccagttg aggcaggttc cgcccaacgc ctctttctca atgatggcgg tttttttgcc
    34501 gagttgtgcg gcacgaatgg cggcaggata accgcccggt ccggcaccga ttacgatgat
    34561 gtcaaagtct tgcatgagaa cctcggggat tggaatggtg gagtgaaggg aagagggacc
    34621 ggtattctct atttatacga gcgatttcca cagttcgatg ttttcgagtt tcgaacggac
    34681 cgcgttagcg aactgcgcgc cgacggcacc gtccacgatc cggtggtcga ccgagagggt
    34741 catggccatc cgggagcgga tgcgcacttc gccgttgacg accgcaggct cttggcgtcc
    34801 gctggagacc gcgagaatgc ccgcctcgcc ggggttgatg atggcggtga actggtccac
    34861 attcagcatg cccatgttcg agacggagaa ggaacttccc gtcatctcat ccggcgtgag
    34921 ccggcccgca cgggcctttt cggccagcgc tttggcttgt gcgctgagtt cactgaacgt
    34981 gagggtctgg gcggcgcgga taaccggcac gaccagaccg ttgtccacgc tgaccgccat
    35041 accgagatcc acatcgccgc gccacgaaac ggtctggccg tccgtgacgg agttgacgat
    35101 cggaatctcc tcaagcgaca gaaccacggc ctcgagcacg aaatcattga cgctatagtt
    35161 caggccgcgg gccttgagat cagaacggaa aaccatcagg tcggtgatgt ccgctttgac
    35221 tgtgacataa aagtgcggga tggtctgctt actctcgacc aaacggcgcg cgatgacctg
    35281 acgcatgcgg gagagggcca cgggtttgcg ccggatcgca cgttcgatgt cctccaccat
    35341 aatccgtccg ctgtcacccg aaccgcgaac cgtcagaatg tcgatgcttt tttctgcggc
    35401 cagccgtttg gccgccgggg tgatacgcag cgcgtcgtaa ccggatgcgg cgagataggc
    35461 ccgcacgtcc ttttccacaa tacggccgtt gggaccggtg ccggtgatgc gtgccgggtt
    35521 gatacaggcg ctctgggcaa gggttttcgc gcgcggcgaa ataacgagac gcgctgcagg
    35581 gacagggacc ttcgcgggct gcggtgccgg atgggaggga ggggcaggca cgggagcgcg
    35641 ggcaggcacg ggcgccggag ccgccgcagg ctccgccacg ggaacgcggg ctgcggcagg
    35701 ggtcggcgaa ggagatgcga caggcttggg cgcgggtgcc ggggcttcgg gcttcggtgc
    35761 ctgatctgga accttttcgc cgggagcgcc gatgtagccg acaacggtgt tgaccgggac
    35821 cgtgaccccc tcgcggacat agatcttcag caggacgccc tcaaagaaac tttccacctc
    35881 aagattcgcc ttgtcggttt cgatctcgaa aaggatgtcg cctttgacga ccgggtcacc
    35941 ttcttgtttc agccattgga cgatggcgcc ctcttccatc gtctgaccga gtttcggaaa
    36001 aatcactgcc tgggccatag cgtctccatt ttttgaaaag taaaaatatc attttagcat
    36061 tgcctgaagc gattgtcatt tgcaaaatca atcaaaatca aaaaaaatcg atcaaaaccg
    36121 agcgcggcca gtttatgctt tctctgcggc cgggaccggg atatcctatc tgggttgccg
    36181 gttggcgtcg gaatccgttc cggtttagtc tgcggacctg tcgaggaggc ggatgattac
    36241 ggttttacat gtgctggagg cgacggaggg tgggacgcgg cgtcacctac gcgatctggt
    36301 cggcgcgcta gagccgttgg ggttccgttg cgccttggcg gtttcgtgcc gccgcgatcc
    36361 ggcctttcgc agggatgtgg ccttctacct gtcgcgcggg ctgacggtgg cggaacttcc
    36421 catgcggcga ggtctggcgc cgattgccga tgtggccgcg ttgttgcgcc tgatcgggtg
    36481 tgtccgccgt gtgcggccgt ccgtgatcca tgcgcacagc gccaaaggcg gtctgctggc
    36541 gcgtgcggca ggtgcggtct gccgggtgcc ggtggtgtat acgccgcacg ttttcccatt
    36601 tctcatggcg tgtgatccgc gtatgcgccg cgtataccga ctcgtcgaac gcggattgcg
    36661 gtgcatgacc gccgcagtga tcgcggtttc ggaggaagaa cggcgcgagg ccgcggcgct
    36721 ggggtatgcg gcgtctcgaa tctatctgat cccgaacgga gtggacggca aggttcccgg
    36781 cgaggtcacg gtgcgtaagg atggggagtt gcgggtgggg ttcttcggac ggctgaccgc
    36841 ccagaagggg ccgtccgtgc tcttggaggc ggttgcggat gtggtggcgc acctgccaca
    36901 ggtgcggttc gtgttctacg gggacgggac ggaggcggac acgttgcgtg cccgggcgga
    36961 agtgctgaaa ctgaacgatg cggttcgctt tctgggatct gtcgcgcagg gagaggtggt
    37021 ggcgcacatg cggcagacgg atgtgatcgg gatcccgtcg cgctgggaag ggtgtccgta
    37081 tgtggttttg gaggctttcc aagccggggt gccggtcgtg gccgcggcag tgggcggagt
    37141 gcccgatctg atccgggacg gggtcaccgg tgtgctggtt gagccggaca atcccgaggc
    37201 gttgtgcgat gcgctgctgt ccctgttgcg cgatccgcag aaacggcgtc ggatcgcgga
    37261 acaggcgcgg aatgaactgg cacgataccg gttggatgcg atggcggctg cggtggcgga
    37321 cgtttaccgc cgcgtcacgc aagatctgcc ctgatgcgtt ttcaggccga acggttttgg
    37381 gttgccagtg actttttcac ttttgcaggg tgctgcacca gcttgcgaac ctcttccggc
    37441 tgcattttcg ctacgacgga atcgtcaacg ccgatcttca ccaaacgggc gcgctgcgcc
    37501 ttcaggcggc ggcgcttctc ggcctgactt ttgatgggac gtgtgcgagg agccttgttt
    37561 aatgtacggc ctgtgcccat gaaaaaaacc tccaaccgat tgctttcagt aatgagataa
    37621 gggcgacatc atggcggatt cgggaggaag atgcaagccc tcgttttaaa cgtgccgcgc
    37681 aacataaaac agcctcactt gtcaaaggaa atgccctgcc gcaatctttc ggggtggaat
    37741 cggcaagccg tataagctat tctaatgcgc tttcaaggag ggtacgcatg tctgtttcgg
    37801 tcgggttcat cagtctcggc tgctctaaga atctcgtgga ttcgcaggtg atggccggat
    37861 atctgaaggc cggggcaata gcgttggcac cttctcccga agaggcggac gtggtgttga
    37921 tcaacacctg cgcctttatc gagagcgcgc gggaggaggc ggcgggggcg attttgcgag
    37981 cctgcgcgca caaggagcgg ggcggttgcc gggcggtggt cgtggcgggg tgcatggtgc
    38041 agcgttaccg gtc
//
LOCUS       DAOSRB010000004        35326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1334_length_35326_cov_6.265147, whole genome shotgun sequence.
ACCESSION   DAOSRB010000004 DAOSRB010000000
VERSION     DAOSRB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 35326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35326
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1334_length_35326_cov_6.265147"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..2510
                     /locus_tag="PLZ74_00475"
     CDS             84..2510
                     /locus_tag="PLZ74_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96829.1"
                     /translation="MNKGTALSYVSMLFATLVAFNTFAQRFTIENHALRRTLTTEGGV
                     LRTEEIENKLNKSVTPLSQAPEFRLRFSEGTHRPETAFTLTSADFELATIKQAPRKDS
                     EPLSFTLRNAKHGITIAVAFSVNATDAFLHKQLTVTSGKPITLERIDVDALTLPDAYQ
                     PYTTREITAQAPARWSPGLGQPLYGAKSATFWGVEFPAADNLVNEKLLLSGYLWGRQL
                     QAGVPYKSYRAVMGVADDPAFVSDAFRDYITQRRVRPLRLQTQFNSWFDRGRGVNKEN
                     FKEAVEKIHKELVVERGCAPLKAYVVDDGWQDVHADWSGKVWKVNSKFDADFASTLTV
                     IREAKSTLGLWLSPGCLFGASAQVDKLRGQGFEALDVWMSMAGPKYMQALEDRVLELT
                     KQGVGFFKLDGIFGHLNRRTFELKGDKYGLPIMPQLGIEGFKSDDKRLNDSKYDELKI
                     YYLTAGTERLMEIFKKQAAINPEVYIVISNGAWLSPWWLMYIDSVWMINAGDAAGGSS
                     RTEELVYRDSIYYKIWTEQKTQFPISALFNHEPKKTGSNESKEVFRKYLYMNLARGTG
                     FVELYITPRNLAAYDWDVIAEGLQWVKEVFPTFSRAKMFGGDPGKREVYGYTGWQGGV
                     GYIALHNPSEEPSDFVCRLDRAFGLQQSERPERFIVSSPLEGSTRGLPDKVQYGDTMT
                     IRLEPREIRIINFDVASRDWSALKTLQTRTQADYVPPPPKPQPKPIPVGNHPLLGVWE
                     YQHNGTTYTREFVAEGNCILKQGNNIIWTKPVTSKDKNTLIVDDCYTHRLQPDGTLVI
                     ENHYKAKRKK"
     gene            2907..3587
                     /locus_tag="PLZ74_00480"
     CDS             2907..3587
                     /locus_tag="PLZ74_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96830.1"
                     /translation="MRIERIMQVCLLSLPLLGAGLLIAAPYASATGRVDAAVSARRWQ
                     AVHDRTTTLRWAYPDGAASATVAITNLSGCEVLRQTFTPPAVTCVWTVCSNEVVTADD
                     YFTVTVSFDVGDALVAGVALLKSSFGGGATVVPEGDRRWGRVGDTALIPYDAAWLDAE
                     TAAFSLARRDGQKSTLEPDCTVGWYGWYAKAWGTGWFDLSLSAAGTEDVLSAAVRRVP
                     PGGIVLIE"
     gene            3643..4869
                     /locus_tag="PLZ74_00485"
     CDS             3643..4869
                     /locus_tag="PLZ74_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOR96831.1"
                     /translation="MKTKTFSILISIAAISAAFAVDVRDVTLQQRWPWSRLIDVEFVI
                     DGEAGSNMRYDVALEAKNGSDVLAVPTAAILGNRSALASGVHVLTIDPVAAGFPDTVL
                     TRFNVTVTASNSPAYMIIDLTTGAKTYTYDWSDTTWCNTDEYKSDKMAFKHIPAGTFG
                     MGYIGTLPSSTDQTNHQVRLTQDYWLGVHEVTQTQWNKLMGTWPSYFSNVTDRAYRPV
                     ENITLTQIRGAWNVARFACPDTSFIGILAARSGLTMDIPTEAQFEYAQRAGVTVNYYM
                     GITSMITAQSYIRFRENAGGYNYDSYQKLDLRNLTIAEGGTADVGHYPPNAWGLYDMA
                     GNLWEVVYDKGGYKDTTAQIDPVHVPDGTVLEIHGGCFNSPFGSWVYNTSRSTWNCTA
                     VKWEIGFRVMAEYEVK"
     gene            4866..7274
                     /locus_tag="PLZ74_00490"
     CDS             4866..7274
                     /locus_tag="PLZ74_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96832.1"
                     /translation="MRTWLLSAVWLAVSVARAAPVQIAAVDCTREFPTNLYFACGDVV
                     VTNGPAGRYRETAPTEASRFGYRFTVRHPGRPHLFVVRYPDDKTRCMAISDGTAYDMS
                     VGVFTGAAKIGNHGRDYSGLAQPFSNTMVEQRNIFWPRWTDASIVFANTTPGQPAAVA
                     SFAIFELDEQDISPLDIPKAVFPRRSFGVSFEDPCSHGADFGAATYADWLDRAVRCMK
                     HQGQNRLIYPLVWYHGPLYPSAIDTAHYFEWSVPSPVDRKIYIRWTSHPADWPEMLLT
                     RFDREGFEFIAQICLLRYGSLMERMNTDAAAVAAGADTVCNVWNDGVVQAGAGDWTGE
                     YNVNNFAVQCAIREKGKRWGNAPLQYGEKIGGNMRRGPIFNPAHPEVRAAVLRNVREI
                     AERYARHRSFKGLKIFTYGSSFLAWPSLACGYDDVSIARFEKETGCVVDAPKAGAERF
                     MARYKTLTAPPLRRVWIDWRCRVVADLVRDIQCTLRNVRPDLEFTVQLNRARECGVDP
                     TLWRGIGVPDVVGFGGVSPCAHKIKPDVDMRDISDVNIYNTWIERWGKHTWWRCAPGE
                     PVPLSMAFLLGQPAEGICRMGSSFEPDGFWWPDAQMRITPAFSGGVHYMRHFANAVAT
                     CDAFTLVRGGLTFDRAHADLLRPFARAFRALPRVKFETLAGTACGNAVVRTQKAEGAR
                     WLYLVNREYVPCDVTLRVEHPRDGLENGCDAVRPAAEWKIHLEPYALRSFAFPSECSF
                     AWTATDVPPTFASDLEKRAATALTACADSADARDKAYVAEVRGLLSARDWPVLRCALE
                     RRQF"
     gene            7296..9428
                     /locus_tag="PLZ74_00495"
     CDS             7296..9428
                     /locus_tag="PLZ74_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96833.1"
                     /translation="MMKMLRSGLILVAVLVARAERVPVFEEGVPQTMCGGASVTAYGG
                     TWIYAAAPDLLDDVSFQQGNATPREKVAPKPAQALKGNYFGVNTTRMPCLNKNLELLE
                     EAVHLIDGDDQTCWLSEAQPRSDMQPIVVRIDLVKETDVAKIVLRKRPLSPETKRRAT
                     DRQPAPDGCEVGRGLPVEMALALSCDAWRWSDVFTGPLGAASEQETFEVAFTPRRAKQ
                     IRLTASKLRQVEFFLYALSLAEIEVLDTQGRNVALVSRGATIAVNATRHQEMPLNMQQ
                     RALWPVQWDLGAKWIRVGYHDDPINWHRVERVKGKLEIDPVADAAISACKEHGQNVIM
                     CLNFGNRLYSGPEKRSFTQLPEWNWCMPNPPATPEALAAWDRYVIFMCRHFRDRVHSF
                     EIWNEWNISVYWGDTPNTEAYCEIVRRTIPLIREHAPQCKVMLGGTSGYFRGLAHQTP
                     EQVAVREEKDSRFVVWKRFAKDVDGIGYHPFYNPNPGWLLDYSADIRAFRAWLARQGF
                     SGTLHASEWNINGRPAPIDPKDARDVWCGIYTQSEIAKAKEVLQQMVRHAGFGIPSCF
                     CEAYNAFYAQTELSLLKGSLLQDPVAPLQPTAAYYALRNAATQMDGFEPAAFTPDVAG
                     TVPGAVAAFAFADGARRGVAVWYEDDQRADTYRQKPVTLTLPFAAAALHAYDPVNGVR
                     QRLNATVDSGKTIVCDLLIGDAPLFVAE"
     gene            9468..10097
                     /locus_tag="PLZ74_00500"
     CDS             9468..10097
                     /locus_tag="PLZ74_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR96834.1"
                     /translation="MLKAMNYEVIPVGPYAANCSVLWDEPGEAWVVDPGEDGDRILAF
                     LEKRGLRAGMVVLTHAHFDYISATNRVLAVYPVKVYLHQNDVAMAFSPLNAMPPYRAT
                     SRPATLDSSKEDGETLTCGGLTARLIHTPGHTPGGWCLYFEQEGLLVAGDTLFAGSVG
                     RTDFPGGSMLALQRSLDRLKRLPDATRVICGHGPMTTIGAEKRGNPYLR"
     gene            10127..11107
                     /locus_tag="PLZ74_00505"
     CDS             10127..11107
                     /locus_tag="PLZ74_00505"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOR96835.1"
                     /translation="MRATDEILTALRQAAGGEVSSRALCDRLGVSRAAIWKRIEALRG
                     LGYGISASPRRGYRLESVPDLPHAAEVLPLLSTRRIGRDCRYVAETGSTNRDAAVLAQ
                     AGAEEGLVVTAGSQHAGRGRMTRVWFSPAGVNLYFSLVLRPAVEPLRAASLPLVAGLA
                     VAEAVADLAPELQPAVKWPNDILVAGRKLCGILCEMQAEIDCVRHIVLGVGVNVNLER
                     ASLPEEIRSRATSLRMACGRSFSRAAVLAAILNHFEPLYDQWLRKGLEPLITRLDARD
                     ALRGRMITLESGGGQTLRGRADGIQADGALRLVTERGPVTLYSGETHIGT"
     gene            11110..11913
                     /locus_tag="PLZ74_00510"
     CDS             11110..11913
                     /locus_tag="PLZ74_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOR96836.1"
                     /translation="MDYRAKQDKTGRVRRIRRWVNGLGRSRQCCVCGRTFFRFSTYRG
                     GWQQFSAYLHNVKWIGSDFDHFWCPFCRSHDRERHLFLYGEKLGFWEQMRGTSILHIA
                     PEKHVSRRIEACKPERYVKGDLVPSREGVERLDVSAIPYAAASFDWVLCNHVLEHVPD
                     DVRALSELFRVLKPGGHAILQTPYASGLERTLENQAEINTDAQRLAFYGQEDHVRLYG
                     RDLFDRIRGPGFSLELIRHADCLGDVDAARYGVNPDEPLFLCVKPLPAV"
     gene            11941..12990
                     /locus_tag="PLZ74_00515"
     CDS             11941..12990
                     /locus_tag="PLZ74_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96837.1"
                     /translation="MRVRIRWVSFWVTLAFCGLMGVSAEAKSKEEPQKVSVLMKEAND
                     LMSQAQSAYVDGDAPKAIELYRKALAEIVRVERENPNRVTSAEFAPVRFRKALCETEI
                     DRILIEEINASSRTVAVSDTRALEEKRKARQEAAATNRVPEATIKLSVKRGVGGGDAV
                     DHPEDKLADAVRSGSEPVAAKTDAPVTKSETGATAKLDVKKELSWVRELLSLEKWDEA
                     DQNLRTVLRHEPTSRDARFLMALVQMRRGRADDASVMMDDLLADPVTDESVCLLASAV
                     YAATGSYGKAMAALDQAMRLNPQRPLGYLNMAWLLLEMRPADLAEPEMYYRQAVKLGG
                     ARDRDIERRLGIKSE"
     gene            13036..16050
                     /locus_tag="PLZ74_00520"
     CDS             13036..16050
                     /locus_tag="PLZ74_00520"
                     /inference="COORDINATES: protein motif:HMM:NF023167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HOR96838.1"
                     /translation="MDARLTKPEPARRWRRLASVVWCGVAVVLSGLLIVDIWLFRVGI
                     VTHMARMTLDRYGFSDTEFAIAGFDTRGVVLEDIRIGQPQPLLEVDQMRLRFRVGDLL
                     RGRLERLDVEGVRLDMRLDEQRITLPLAELLLPRLATIRRPDRKSARIFQLDAATVRE
                     MRIDLLNRKEVPLTQLVLELSAFAEPHGCTRVWGSLGDAATNLCVRVDGTLEPGTGNL
                     SLRSDMRVATLAPVADWIMQATAGEGRRHRIPVRIEGGLTARGEVTAERWREMRTGNM
                     EVALDRQTVIRFEGEDRFVRFQNLRADLTGAPDEWSLRLHAGVSGFRLDAERAVARES
                     GRLLSLRGNARLQRTGRRQHVTATLESDLPGRSLGQLFSAMLPLVPRLFAEGGTLRVE
                     TDLTAEQGGKWTGQTEFLATAKHSSVRVPAGRAGAESVSVSATVPMEKGKAGALQARV
                     AVEDGFVFKKGFVLRGGAEMRLTAAPPYDTANGTLTGRIAEMRALRKRGVSMSQEGVG
                     FESAVQVSGLVTNPVWHLDLHMPETAVAVTNPVCHASAMMGAQARVQYGAQVLAAEGG
                     VWVRDGAARLTVSGGVQNVVAGLERAALRFDLPAYAVSAPDQAVADVSVEVTNGWLRA
                     DDRLALEGLQMFVPLRGSVRDGITCLAGQRFGWDHWEAEGVRLVADGFGVEADTGQIA
                     GRLGVRAVDSAARVDIGVVVPIARPGQAKATVTFPEASLERDDALAARLRKAVPDLEA
                     SARVAAEAAFRLGGGTPHAVGRVCVRDGQFRKKEMSVSGLALDMPFEFSPTFRTIERP
                     SLTFKQIQAGNIRLDAGRIEFDLTPREVSVGSMEVGWCKGSLRAYSVLFDLKDRKDEF
                     IVYADRIDLGEALMMVMPFQGVMEGVLYGRFPVGIDGNRIRLSTGYLYSLPGQGGRLK
                     LDDAAPMASLLDRAGIKGDVQRPLSKALSDLLFHAIRLELEPKPEGDAVLRILLEGKS
                     NDKDWPAPVSLNLNLHGPIEKLVNVGLDLSRE"
     gene            16066..16239
                     /locus_tag="PLZ74_00525"
     CDS             16066..16239
                     /locus_tag="PLZ74_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96839.1"
                     /translation="MRPKERRFLSVLLLVVTTGCITGCFWVKTEHEVKPIHITMDVNL
                     KVQKELEDFLCLD"
     gene            16259..16600
                     /locus_tag="PLZ74_00530"
     CDS             16259..16600
                     /locus_tag="PLZ74_00530"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HOR96840.1"
                     /translation="MRNKWRVRGLMLACVLGCVCAAQADTMQEIKARRRARREQIRQL
                     VQTGHAKEGDEGYLVAQRALTADQTALMQAENEDRKAGYTAIAQANGKSVAEVGRQAA
                     VIQKNRRASKQ"
     gene            16651..18015
                     /locus_tag="PLZ74_00535"
     CDS             16651..18015
                     /locus_tag="PLZ74_00535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOR96841.1"
                     /translation="MRTRFRWVWMTVVLSAGMVFGRMFRDGEVVCFLGDSITNGGRYQ
                     NTVWDYYLTRFPGWTIRFVHAGRSGDSAGGSLGRLQEDVMDYKPTSVAIMMGMNDVGR
                     GNYVANPDEKRKAAQQRSLDGYKANMEKVTERILNEANRPHLFLITPSPFDQTVVLER
                     DNNQPGCNDGLGRCAAIVRELASKVNATVVDFHGPMTAFNLEQQKKDPKYTIVGADRV
                     HPGAPGHLMMAWLFLKAQGVPSLVSRVAVDASAGQVTAGENATVTAVAKRDGGVTFTV
                     LAKALPFPTDPAACKALEGLLPILEELSQETLHVTGLAAGEYALVIDGKEVGRYSAAA
                     LATGISLGANTMTPQYQQALNVSRLNARRRDAECQARNLLNTRRWMKNHHKINVDDPA
                     AVQAHYDGMKDKRQYSAVMALNYIKKWPQYEAYRKNAAELEKEMLASRTPAPHVYEVR
                     PVGR"
     gene            18195..18269
                     /locus_tag="PLZ74_00540"
     tRNA            18195..18269
                     /locus_tag="PLZ74_00540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18228..18230,aa:Glu,seq:ttc)
     gene            18451..19674
                     /gene="lpxK"
                     /locus_tag="PLZ74_00545"
     CDS             18451..19674
                     /gene="lpxK"
                     /locus_tag="PLZ74_00545"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOR96842.1"
                     /translation="MRKVKIRRIERIEHYLVRLIQQPGDDQGHPWPIRTLLAFLKGLS
                     EVFKIIVSLRLFLYEVGICRRYPLGCQVISVGNITAGGTGKTPVVEIFARELQRSGRK
                     VAVLSRGYRKKELPWYQRLFREQIEPPRVVSDGKRILLDSEMGGDEPYMLASNLPGVV
                     VLVDRNRVKSGRYAVKRFGCDTLILDDGFQYQKLKHRLEVVLVDKTNPFGNSQLLPRG
                     VLREPVRNLQRADFIFITKSDGDSDALRARLHELNPTAEIIECRHRARYLQHVFTAER
                     LDLAWLKGRTVTSLSGIAVPQSFENSLRTMGARVIHCERYADHHRYHAQEIINAVNRA
                     ADLKADALLTTEKDAVRFPRLEVTPVPVYYLRVDIELMRGAENFHEAVERICFRPPPR
                     ASAARAQSVPVAEKT"
     gene            19685..21319
                     /gene="murJ"
                     /locus_tag="PLZ74_00550"
     CDS             19685..21319
                     /gene="murJ"
                     /locus_tag="PLZ74_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOR96843.1"
                     /translation="MSVSQTEQKGPHPRVLRSVSVVSLMTAVSRVLGLVREVTMAYFF
                     GTSALKSAFDIAFIVPNLFRRLFGEGALSSAFVPVFGETLIREGHASAFRFGMRVISM
                     LTVVLGFITTAGILLSFPLARMLPPESRWLIPLPMLRVMLPYALLICIAALLSGMLNT
                     LGKFAIASLTPFLLNLIWIVTLLVVCPFVFRAPEQQIQTLSWAILLAGLAQILFQLPA
                     LGRHGFHFRLRFRGMFHDPGVKRVLYLMGPASLGIGLIQINVCIDKVLAFWADPAAPA
                     ALEYAERLIYLPLGVFGTAFMTVLLPTFSRQASAGELPAMQATLERSLRNLSVIMAPC
                     SAALFVLALPVILLVYSFRGGRFDETSALLSARALAAYAPGLLVFSFQKAITPAFYGL
                     QDLRTPVRVSLCALVLNITLNVFSVLWLPHGWKHVGIAGSTVLTSLVNAVALVILLRR
                     RTVAPRREAVIPTLLKAVGCSLVMAVGVWRVCTVGMRWADGLGEYVKGAQIGVMALTV
                     LCGVILYGGLMVWVGRGAVWEMVGEFRRRPVKGVRQ"
     gene            21316..22380
                     /locus_tag="PLZ74_00555"
     CDS             21316..22380
                     /locus_tag="PLZ74_00555"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOR96844.1"
                     /translation="MKPKTFVVACGGTGGHVFPGLAVAQELRLRGHAVDVWFSGRAVE
                     RSTLCDWDGPVFLTGARQLSVRTLPQTLNAFFRCARELKRRRPDALLAMGSYTSLPPV
                     VAARLANVPILLHEANAVPGKAVEVLSGMAELTAISFEETAAWMPRRKTVFTGLPVRQ
                     ELIGKPRFDEIPEGMFTVLVTGGSQGARRVNRLVTQAFCLLQQSGSGEFAVLHQSGAA
                     DEARVRQTYEEAGVWARVRAFEPEMGRAYASADLVVCRAGASTCFELCLLGKPALMIP
                     LPSAVRNHQFLNASALARSGGVDVGIEKELSARSIMRYVQNKMRTPACLMHMGEALKA
                     LAHPAAAQRVADALESLSEA"
     gene            complement(22439..24109)
                     /locus_tag="PLZ74_00560"
     CDS             complement(22439..24109)
                     /locus_tag="PLZ74_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96845.1"
                     /translation="MNTKRAKFIVRFPSALRMAGLSLAVLLPCVSRAERLFVGILETD
                     SYQSVIYGASAFSRLADLPLALEWVQGALDRATCLPSFTGVDRNRPLRIVQTVETQKP
                     VGAGNPANVAIIPLTDDGRTVLDALAAAYRKQTRVEPFTRFEEPASTNLTPVVTVVVV
                     KRHLFTSSSAEAVLWAWENRSLLLDAPPQSIPGTLRVLVNPQRLADLTGVRSDKAGAF
                     VNTDRFLRECETFSFALTFESQALTLTVRGRPTAGTGLARLVAAMRPPRATLWHGVPD
                     RAFFASVSACDAPQLWDPYLGSQRLSLVRPYAGIAPTNGFSGDCLISLSPTRDQRGIC
                     LVQIEPVVQTADAEKAIRSLHTVSHADGITLKPLPARRAATGVEIVSYRLAFHAPEAE
                     AEGKTDVSILNTLLELFLKQAVLETAVTEGFVITTVGPVQTLDGELTELTFRERQLTL
                     ERRLRMQDPALADTPLTSGANLRLASLMRHLVSIIPNVKPEHLRPFPTGGDGAALGLG
                     RGANGAFTASLRFGTAELAALQRINRDGRAVLQELFFQMFSSQMMGTQ"
     gene            complement(24106..24615)
                     /locus_tag="PLZ74_00565"
     CDS             complement(24106..24615)
                     /locus_tag="PLZ74_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96846.1"
                     /translation="MRRHAWAAAALVIVCGCQHPMENPYFTVTESGLNWVDIRHYRIG
                     NPTQRVWVRLDGNGIVTVREGSSPLVSNPFAKDMTHQQWYDIRETRLTIPRHEAVFLF
                     QTLVDKGLFEKPVKPKNIEEADQIYVSANIQNKTVSYVDPIGDPDLAEQCRLLVMMFH
                     HPKPRARKP"
     gene            complement(24620..25861)
                     /locus_tag="PLZ74_00570"
     CDS             complement(24620..25861)
                     /locus_tag="PLZ74_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96847.1"
                     /translation="MHLTRHSLSISLLISLLTAHANAEPFPVFSAARAITSGPKDHLF
                     ASYYAINAWSANNRYVTVLETDVKNRLPTEADVAELGVVDLQDNNRFIPLTTTRCWNF
                     QEAAMAHWLATAPDRHFLFNDLVDGRFVTVIFDLQTRTRRIVPFPVSAVSPSGKEAVS
                     INYARLRLTRPDYGYGGKGQDARETVSFPQDDGLFLVDLETGTAKLIVPIASVREQMP
                     SVEEGVDLAYFCHTVFSRDGSKIFWLARSVNPKTARNWKTTSFTCNRDGSGVRRCFPD
                     GWGGSHFNWLDGDRLMVTANYQKKVYTHVLFTVGKTDYTRVGRGMLDFDGHGVFSDDG
                     LWMATDTYPDRLNERKLMVLRMADHAILPLGGFFVPESYRGTYWRCDLHPRWRPDGGM
                     LAFNSVHEGSRQVYVMDVKKR"
     gene            complement(25842..26900)
                     /locus_tag="PLZ74_00575"
     CDS             complement(25842..26900)
                     /locus_tag="PLZ74_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOR96848.1"
                     /translation="MNTPPLTPYTTTLTDAARRRGIRVTMINPDPELPIFTLSREGRS
                     VRCFNALTDHVGAATYHLLNSKRACHRWLSRHRIPVPTQCVYRDAQPEEALAFLRTHA
                     PVVVKPDSQWGGRGVAMGVRTPAELHAAIRRARRYERDVLLEETVPGEDLRVILAGGK
                     LAAAIRRKPAQVTGDGRHTIRQLITLHNRRRRAEDPSNLIPWDPETRHNLAEQGHAPD
                     DVPAAGCAVQVRLTHNYHTGGTVELATHAVPAYALRLAQRIVRALPVPLIGVDFLIDR
                     ATRRATVVEVSPDMAISPPEGAQVAECFLDVLFPETVTGNATCCRPYPLTAKHGKKTC
                     GTTHAIKMKGSDHASDTP"
     gene            complement(26897..27148)
                     /locus_tag="PLZ74_00580"
     CDS             complement(26897..27148)
                     /locus_tag="PLZ74_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96849.1"
                     /translation="MRMSRFLLLSVVPVLAFGTAAVEVTFIPHRIRTNRTEACGIADF
                     HGDGKLGIVVADMDGNGDWDLVTTGKWGGPVLIENRLKR"
     gene            27348..30614
                     /locus_tag="PLZ74_00585"
     CDS             27348..30614
                     /locus_tag="PLZ74_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96850.1"
                     /translation="MKRKRMMVVGAVVFGVAAVRAADVYWWKGSAGDHKWSTADNWAV
                     GSLEGDTATEAPTNGQDVWLVGYAGETIINDVDGVTLNSLHTTNQVDTTHPVMLIGKP
                     LTLSATYKCYTRTTNEVGLVFTTTSAVSIYGANNYNVIRGGISAENAISLTFSNARDL
                     GYDCPIFNVYGAVNVPKGSVRAAQGNSATSFYGPVTAVTLYSGQYYGNGHVHLYAIGN
                     NIGTVYASKYNICNFNAANCFVNNPLLCWDWNNNTDSNGETFRFYGDLTIDHFESTTL
                     QSAEVSNVKRAIGAASGISTVTMKATTNSNTSASYNDKLSLVWDPQGDYTFMNTNRIS
                     TMTGTITVKRGRMVVAGTANFTKLTGITVEDGAAFEAASTSAATTFPLLTTLTLGRNA
                     RFDVTNTTSTVFAQDVTLLVLAEGAKLYIGRDIELKFTGVNRDGVYLASTNFSAATCD
                     WIEGEGTVTTPAVSTLTSWKTPADGDWSTEANWSHGVPAAGKETYLTIQGGTYTARVD
                     VASLVNGRTTLGNTDGNESALLVGNDLAIADGNWLQINAGGAYVQTNGVSEFRNTAEA
                     RRTTVNEGGLWRLEGTATNRFLTSDSGFFTLAGGRIEIADQAELYFENIQHAYNQYSA
                     LDAGRIDISGNGVLKYSSAQNSMANNIRDAEIIVRDNAKFVFTYASFASKANGEFRLV
                     VTNNASYRASACTYVGYNGYSAVPSHCRQIFDFHTDVSPSNYFGWTGFVGVNASGYSE
                     VNIHEGAMVQLGGGYGCRIGACSGKSDKSVCSPTGVVNVAGTARVEGNYYSTYIQQNK
                     AGYNAFYGLLVGGGYQLYYEDIYNNRVHRELPTDSRMKGIVNIAPTGSFLLWQGMLAV
                     GIGTGGDGEINIDGGTFTKARDGNTAIGMGGGKGAVTITGGGRMDAYGTLFIGGVRTN
                     DFESVVYYDDWYVDDGAPASLRDAEGTLRVVNGVYRQAGGAAKIVVGAEGTGMIEIGS
                     NGVVQAEHVVMSNGVQSVLRFVAGANGFGYLDAQKSLSICDGQRVEVDLTEYAGSKTK
                     HQLVKFSGTNENFRVDVLQLDAVFTDESGKNQHKVAVLRKGADGLYLNLMQGTVLHFK
                     "
     gene            30657..33713
                     /locus_tag="PLZ74_00590"
     CDS             30657..33713
                     /locus_tag="PLZ74_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96851.1"
                     /translation="MTLLGTLVFAVVHLGAVETVIDAAETPQIEKETVLAVGDVLKLV
                     GKKADFVATAPIRGAGATLYGALGSPGSCTHQAVFEAPIEFDTVLWGQDYGESKLVLK
                     ASGNRWHKFELAYAKVECAAANVLAPEGVLTWGDYYRGTLDRNATFDLGGFDQTIDRL
                     HSTSSPGTKNSDHVVSTGGTLLMRGTADAVCFARIDGELNLVWHPTGDFTQSFDERVN
                     SVRGRIEVRRGTVRLGKSGAFRNIDEIKVADGATFVVEATNALALAAVKLVELGKDSA
                     LVVKGVDPFMPQQVSVVKDVGAKIESAVPLKLAREAAYDDTPPRTIRDKLQVFWDDWI
                     VDPAMTSARRVPHHPEYVGPVITTDKPWEGASGSYMSVVKVDDVYRMYRQGMSSHESE
                     PHYHGLKICLFESKDRGLTWERAKINRFPYKGNRENNIVIDFRVNYWDNFMAFRDDNP
                     ACPEAERYKAIALYSHKWSEEFAKAHPDYAPLAKYGKALWCFVSADGLDWKPGWPLFK
                     FGDMGLRFDSLNVAFYDEVNREYRMYMRNVKPLEYERYGGTNARWVMVTRSKDFHNWS
                     KPENIDFNDGQEEYELYTSGVDLYPRNPELTVGFPTRYTDRRRWSKNFERLCGRNKRV
                     ERLAPDPRHGIAITDTVFMWSRDGKTFTREEDAFFSPGPEHAGNWMYGDGYLQRGFVV
                     SPGRYGSDDELSFYEVDGGWTGSRLTINRYALRMDGFVSYHGRYRTDKLVTKRVVFDG
                     NRMLLNFKTSARGRVFVKITDEAGKSIVTDEIFGDKVDRIVDFEDGEVSAFAGRPVRI
                     EFGLSDADVYSFRFEKAADAGARDACPYRGRAGEYKTEIEKTCWSNMTDEVRIPYLAA
                     LSMEYRVTAERETMLKADELFMVLKDTQRLNATVTEGQPLFGELLAVVDCHWRIARDP
                     WQIRWFYSRLGACDHPLALAFNVKYSRNDQAKAKLKELLAAGRVRPEELPVTWDRLNN
                     EDGYVQERFADRLVKDFDLTSQPQDAAGKARWLWAYWTMRLLNNGR"
     gene            33866..35146
                     /locus_tag="PLZ74_00595"
     CDS             33866..35146
                     /locus_tag="PLZ74_00595"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOR96852.1"
                     /translation="MPNTVLVGAQWGDEGKGKVIDVLTSQADLIVRYQGGSNAGHTVI
                     ADGRKYVLHLIPSGILHTDKQCVIGNGVVLDPFDLIREIEGLREQGFDPLGRLYISDR
                     AHMVMNWHRALDAADEARRSQERKIGTTGRGIGPAYGAKIGRSGLRAGDMLSPSFLEM
                     VEAGVAEANTVLAGWGAPLLDGRETRVRYEEAVRTLTPYIADTVTLVNEADRAGKSIL
                     LEGAQGTMLDIDFGTYPFVTSSSPTAGGACTGSGISPRRIDRVVGVIKCYTTRVGAGP
                     FPTELTGETGERLRQKGGEFGATTGRLRRCGWFDAVVGRYAAMVNGVDFWAMTKLDVL
                     DEEPVIRICTAYERDDGVRYEIFPSDLSVLERCTPVYEEMPGWLTPTSCCTRYEDLPL
                     KARAYVERLIALIGGRLGILSVGPAREATLRLGL"
     gene            35175..>35326
                     /locus_tag="PLZ74_00600"
     CDS             35175..>35326
                     /locus_tag="PLZ74_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOR96853.1"
                     /translation="MSVDTQTNRVPSHIAIIMDGNGRWARQRGKPRLAGHEAGSETVR
                     CVLNYCR"
BASE COUNT         6933 a         9736 c        11514 g         7143 t
ORIGIN      
        1 tcaacgttga aatcgttagt ggggaaagtt ccttgttgta agaccttttc ttcagcggtg
       61 gcgtttgtta tcatgcccac acaatgaata aaggcaccgc gctatcgtac gtctccatgc
      121 tcttcgccac cttagtggct ttcaacacct ttgcacagcg cttcacgatt gaaaatcatg
      181 cgctccgtcg aacgctgact acagaaggag gggttctccg gaccgaggag atcgaaaaca
      241 agcttaacaa gagtgtcact cccttgagcc aggcccccga gttccgtcta cgcttttccg
      301 agggcacgca ccggccggaa accgccttca cactcaccag cgccgatttt gaactcgcca
      361 cgatcaaaca ggcgccgcgc aaggactcgg aacccttgtc ttttaccctg cggaacgcaa
      421 agcatggaat tacgatagcg gtcgcctttt ccgtgaatgc gacagatgcc tttttgcaca
      481 agcaactgac cgtgaccagc gggaagccga tcacgctgga acggatcgac gtggatgcgt
      541 tgaccctccc cgacgcctac cagccctata cgacgcggga gatcaccgct caggcacccg
      601 cccgctggag tcccgggctt ggccagccct tgtatggcgc aaagagcgcg accttctggg
      661 gtgtcgagtt cccggcggcg gacaacctcg tcaacgagaa gctcctcctc tccggttatc
      721 tctggggtcg ccaactccag gcaggcgtgc cctacaagag ctatcgtgcc gtcatgggcg
      781 tggcggatga tccggccttt gtcagcgacg ccttccgcga ctatatcacg caacgccggg
      841 tacgccccct gcgcttgcag acgcaattca actcctggtt cgaccgtggc cgtggcgtca
      901 acaaggagaa tttcaaagag gcggtcgaga agattcacaa agaactggtg gtggaacgcg
      961 gatgcgcccc gctaaaggct tatgttgtcg acgacggctg gcaggatgtc cacgcggact
     1021 ggtccggcaa agtctggaag gtgaacagta aattcgacgc cgacttcgcc tccaccttga
     1081 cggtgattcg cgaggcgaag tccacacttg gcctctggct gagccccggc tgccttttcg
     1141 gggccagcgc acaggtcgac aagctgaggg gccaaggttt tgaggcgttg gatgtctgga
     1201 tgtccatggc gggccccaaa tacatgcagg cccttgagga ccgcgtgctc gaactgacga
     1261 aacagggcgt cggtttcttc aagctggacg gcatcttcgg ccacttgaac aggcgcacct
     1321 ttgaactcaa gggcgacaaa tacgggctgc caatcatgcc ccaactcggc atcgaaggtt
     1381 tcaagtctga cgacaaacgt cttaatgact cgaagtatga cgaactcaag atttactatc
     1441 tcaccgccgg cactgaacgc ctgatggaga tctttaagaa gcaggctgcg atcaatccgg
     1501 aggtttacat cgtcatctcg aacggcgcct ggctcagtcc ctggtggctg atgtatattg
     1561 actcggtttg gatgatcaat gccggcgatg ccgcaggcgg aagttcacgt accgaagaac
     1621 tcgtctaccg cgacagtatc tattacaaga tttggacgga acagaagaca cagttcccca
     1681 tcagcgcact cttcaaccat gagcctaaaa agaccgggag caacgagtca aaagaggtct
     1741 tccgcaaata cctctacatg aacctcgcga gggggacggg attcgtcgaa ctctacatca
     1801 cgccccgtaa cctcgccgcg tatgactggg acgtcatcgc cgaaggcttg caatgggtca
     1861 aggaggtctt ccccacattc tccagggcaa agatgttcgg gggagacccc ggcaagagag
     1921 aggtctatgg atatacgggc tggcagggtg gggtcggcta tatcgccctc cataaccctt
     1981 ctgaagagcc aagtgatttt gtctgccgtc tggatcgtgc gttcggctta cagcagagcg
     2041 agaggccaga gcgcttcata gtgagttcgc ctctcgaagg ctcaacgcgc ggcctgccgg
     2101 acaaggtgca gtacggcgac accatgacga tccgtctgga accgcgtgag atccgcatca
     2161 tcaactttga tgtggcgtcc cgtgactggt ccgcattgaa aacgttgcag acccgcaccc
     2221 aagccgacta tgtcccgcca ccgcccaaac ctcaacccaa gcccatccct gtcgggaatc
     2281 atccgttgct tggcgtatgg gaatatcaac ataacgggac gacctacaca cgcgaatttg
     2341 ttgcggaagg gaactgcatc ttaaagcaag gcaacaacat catctggacc aagcccgtca
     2401 cgtcaaaaga taaaaacaca ctcattgttg acgattgtta cacgcatcgg ctccagcccg
     2461 acggcacact ggtgatcgag aaccattaca aggcgaagcg taaaaaataa acctgttctg
     2521 aataccatca tgaacacaaa cttcgcgcct gcgcgggaga aaaaagggca gctgaacttt
     2581 caaccgcaga ttgacgcaga tgggcgcaga ttttgggggc cggctgagtt ttgcacgaaa
     2641 caggcacgtc gccgctcctc tgaccatgac gcattcttaa cctgaccgac gaatgcgaaa
     2701 tgccggaatt tgcgtttttt tgacattatt ttttgcgcaa atctgacttc cgtaaaacag
     2761 gtcgggagcg tatagtggta atgttaagaa caggtccggg tcaaagcaga acgggaggtc
     2821 gccttgtgaa cgctccgcac cggttccttt cataaaagca acgtgaatgc ggcccgccgc
     2881 atcccgaaat gaggaggcca aagacaatga ggatcgaacg tatcatgcag gtatgtttgc
     2941 tgtccctgcc cttgttgggg gccggcctgt tgatcgccgc gccgtatgcg tccgcgacgg
     3001 ggcgggttga tgctgccgtg tccgcgcggc ggtggcaggc cgtccacgac cggaccacaa
     3061 cgctccgctg ggcgtatccc gacggcgcgg cgtccgcaac ggttgcgatc acgaatcttt
     3121 ccggatgcga ggtgctccgg cagacgttca cgccgcctgc ggtgacatgt gtctggacgg
     3181 tttgctcgaa tgaggtggtg acggcggacg attatttcac tgtgacggta tcgtttgacg
     3241 tcggcgacgc cctcgttgcc ggcgtcgccc tgttgaagag ttccttcggt gggggcgcga
     3301 cggtcgtgcc ggagggcgac aggaggtggg ggcgcgtggg cgatacggcg ctcatcccct
     3361 atgacgccgc gtggctcgac gcggaaacgg cggcgttctc gctggcgcgg cgcgacgggc
     3421 agaagtcgac cctcgaaccg gattgcacgg tggggtggta cggttggtac gccaaagctt
     3481 ggggcacagg ctggtttgac ctctcgcttt ccgccgctgg tacggaagat gtgctttccg
     3541 ccgccgtgcg gcgggttccg cccggcggga tagttctgat cgagtgaaaa cgcgaaagaa
     3601 agtccacaaa cgattccagc acagccaaat caggagataa tcatgaaaac caaaaccttc
     3661 tctatactca tttcgattgc cgcgatcagc gcggcgttcg ccgtcgacgt cagggacgtc
     3721 acgcttcaac aacggtggcc gtggagccgc ctcatcgatg tcgaattcgt catcgacggc
     3781 gaagcgggca gtaacatgcg ctacgatgtg gcgctcgaag cgaaaaacgg cagcgatgtt
     3841 ctggccgtgc cgacagcggc gatcttgggg aacaggagtg ccttggcgag cggtgtgcat
     3901 gtcctcacga tcgaccctgt ggcggccggt tttccggata ctgttctcac ccggttcaac
     3961 gtgacggtga cggcgtcgaa ttcgcccgcc tacatgatca tcgatctgac cactggtgct
     4021 aaaacctata cctacgactg gtctgacacc acgtggtgca atacggacga gtacaaatct
     4081 gacaagatgg cgttcaagca cattccggcc ggcacgttcg gcatggggta tatagggacg
     4141 cttccctctt cgactgatca gacgaaccat caggtgcgtc tcacgcagga ctattggttg
     4201 ggcgtccacg aagtgacgca aacccaatgg aacaagttga tgggcacttg gccttcatat
     4261 ttctcgaatg tcacagatcg tgcctaccgt cccgtcgaga acatcaccct gacgcagata
     4321 cgcggtgcgt ggaacgtggc gcgtttcgcc tgtcccgaca cgtcgtttat cggcattctg
     4381 gccgcacgca gtggcctaac gatggatatt cccactgagg ctcaatttga atatgcccag
     4441 cgcgcgggtg tgaccgtcaa ctactacatg ggaattacct ctatgatcac ggcccagtcg
     4501 tacatccgtt tcagggaaaa cgccggtggt tacaattacg actcctacca gaagttagac
     4561 ctgcgcaatt taacgatcgc cgaaggcggc acggccgatg tgggtcatta tccgcccaat
     4621 gcgtggggat tatatgacat ggcgggtaat ctttgggagg tggtctacga taaaggtggt
     4681 tacaaagaca cgacggcgca gatcgaccct gtccatgtgc ccgatggcac ggtgttagag
     4741 atccacggcg gttgtttcaa ttcgcctttt gggagttggg tgtataatac ctcgcggtca
     4801 acttggaatt gtacggcggt aaaatgggag atcggcttcc gtgtgatggc ggagtacgag
     4861 gtgaaatgag aacgtggctt ctgtccgccg tctggctggc ggtttccgtc gcacgggcgg
     4921 caccggtgca gatcgcggcc gtggactgca cgcgcgaatt cccgacaaac ctctatttcg
     4981 cctgtggcga cgtggtggtg acgaacggcc ctgccgggcg ttaccgcgag acagcgccga
     5041 cggaggcctc gcggttcggt taccgtttca cggtgcgcca tccggggcgt ccacatcttt
     5101 tcgtggtgcg ctatccggac gacaagacgc gctgcatggc gatttctgac ggcacggcat
     5161 acgatatgag tgtgggcgtg ttcacgggtg ccgcgaagat cggtaaccac ggccgagatt
     5221 attccggact cgcccagccg ttttcgaaca cgatggtcga acagcggaac attttctggc
     5281 cgcgctggac ggatgcctcg atcgtgttcg ccaacacgac gccggggcag cccgccgcag
     5341 tcgcgtcgtt cgcgattttc gagttggacg agcaggacat atcgccgctg gacatcccga
     5401 aagcggtctt cccgcgtcgc tccttcggcg tgagctttga ggacccgtgc agccacgggg
     5461 ccgatttcgg agccgcgacg tatgccgatt ggcttgatcg tgccgtgcga tgcatgaagc
     5521 atcagggaca aaaccggctc atctacccgc ttgtctggta ccacgggccg ctttatcctt
     5581 cggcgatcga tacggcgcat tactttgagt ggtccgtgcc gtcgccggtg gatcgtaaaa
     5641 tctacattcg ctggacgtcg catccggcgg attggcccga gatgttgctg acgcgtttcg
     5701 accgggaggg gttcgaattc atcgcgcaaa tctgcctgct ccgctatgga agcctcatgg
     5761 aacgcatgaa cacggatgcc gccgccgtgg cggccggcgc tgacacggtc tgcaatgttt
     5821 ggaacgatgg cgttgtgcag gccggagcgg gcgactggac cggcgagtac aacgtgaaca
     5881 atttcgcggt gcagtgtgcc attcgggaaa aagggaaaag atggggaaac gcgccccttc
     5941 aatatggaga aaagatcggc ggcaacatgc ggcgcggccc gatcttcaat ccggcccacc
     6001 cggaggtgcg ggcggccgtg ttgcgtaacg tacgtgaaat cgccgaacgt tatgcgcggc
     6061 accgttcgtt caaagggtta aaaatcttca cctacggcag ttcatttctc gcgtggccct
     6121 ctctcgcatg cggctacgac gacgtgtcca tcgcacggtt cgaaaaagaa acaggctgtg
     6181 tagtggatgc gccgaaggcg ggcgcggagc ggttcatggc gcgttacaag acgctcacgg
     6241 caccgccgct tcggcgtgtg tggatcgact ggcgttgccg cgtggtggcg gatctcgtgc
     6301 gtgacatcca gtgcacgctc cgcaacgtcc gtccggatct ggagtttaca gtgcagctta
     6361 atcgggctcg cgagtgtggt gtggacccaa ccctctggcg cggcatcggc gtgccggatg
     6421 tcgttggttt cggcggtgta tccccttgcg cgcacaagat caagccggac gtggatatgc
     6481 gcgatatctc cgatgtgaac atctacaaca cctggattga acgctggggc aagcatacgt
     6541 ggtggcgttg cgcaccgggt gaacccgtgc cgctcagcat ggcgtttctt ctcggacaac
     6601 cggccgaagg catatgccgg atggggtcgt cctttgagcc cgacggattt tggtggcctg
     6661 acgcacagat gcgcatcacg ccggcttttt cgggcggtgt ccactatatg cgtcatttcg
     6721 cgaatgccgt tgcgacatgc gacgcgttca cgcttgtgcg cggggggctc acgttcgacc
     6781 gggcgcacgc ggatctgctg cgcccgttcg cccgcgcgtt ccgcgccctg ccgcgcgtga
     6841 agttcgaaac actcgcgggc acggcgtgcg gcaacgcggt cgtgcgcacg caaaaggccg
     6901 agggtgcgcg gtggctctat ctggtgaacc gtgaatacgt gccgtgcgac gtgaccctgc
     6961 gcgtggagca tccgcgcgac gggttggaga acggttgtga tgccgtccgt cccgccgctg
     7021 aatggaaaat ccatctggag ccctatgcgt tgcgcagttt cgctttcccg tccgagtgtt
     7081 cgtttgcatg gacggctacg gacgtgccgc cgacgttcgc aagcgatctg gaaaaacgcg
     7141 ccgcaaccgc gttgacggca tgtgcggatt ccgccgatgc gcgggacaaa gcgtatgttg
     7201 ccgaggtacg cgggcttttg tcagcgcgtg actggcctgt tttgcgatgc gctttggagc
     7261 gtcgccagtt ctgataaaaa aggaaggaca cgacaatgat gaagatgctt aggtctggtc
     7321 ttattctggt ggctgtgctt gtggcgaggg cggagcgtgt ccccgttttt gaagaggggg
     7381 tgccacagac gatgtgcggc ggcgcaagcg tgacagccta tggcggcacg tggatctacg
     7441 ccgccgcgcc cgatctgctc gacgacgttt cgtttcagca gggcaacgct acgccgcgcg
     7501 agaaggtggc acccaagccc gcgcaggcgc ttaaaggcaa ttacttcggc gtgaacacga
     7561 cgcgcatgcc gtgtctgaac aaaaacctcg aacttcttga agaggcggtg catctcattg
     7621 acggggatga ccaaacatgt tggctgagcg aggcgcagcc tcgaagcgac atgcagccca
     7681 ttgtcgtgcg catcgacctc gtgaaggaga cggatgtggc gaagatcgtc ctcaggaaac
     7741 gtccgctttc gcctgaaacg aaacgccgtg ccaccgaccg gcagcccgcg ccggatggtt
     7801 gcgaagtggg gcgcggcctc cctgtggaga tggcgctcgc cctctcatgc gacgcatggc
     7861 gttggtcgga cgtctttacg gggcctctcg gcgcggcgtc cgaacaggaa acgttcgaag
     7921 tggcgttcac gccgcgtcgt gcgaagcaaa tccggctcac agcctcgaaa ctgcgccagg
     7981 tggagttctt cctgtatgcg ctgtcgctcg ccgaaatcga ggtgctcgac acgcaggggc
     8041 gcaacgtggc gctcgtctca cgcggtgcca ccatcgcggt gaacgccacg cggcatcagg
     8101 aaatgccgct caacatgcag cagcgggcgc tctggcccgt gcagtgggat ctgggcgcga
     8161 agtggatccg cgtaggctat cacgacgatc cgatcaactg gcatcgcgtg gaacgcgtca
     8221 aagggaaact ggaaatcgat ccggtggcgg acgccgcgat cagcgcctgc aaggagcacg
     8281 gccagaacgt catcatgtgc ctcaatttcg gcaaccggct ctattcgggc ccggagaagc
     8341 ggtcgttcac gcaactgccg gaatggaact ggtgcatgcc caatcctccc gcgacgcctg
     8401 aagcactcgc ggcgtgggac cgctacgtca ttttcatgtg ccgccacttc cgcgaccgcg
     8461 tacattcgtt cgaaatctgg aacgagtgga acatctccgt gtactggggc gacaccccga
     8521 acacagaggc ctactgcgaa atcgtgcgcc gcaccattcc gctcatccgt gaacacgcgc
     8581 cgcaatgcaa agtgatgctc ggcggcacga gcggttactt ccggggtttg gcgcatcaga
     8641 cacccgaaca ggttgccgtg cgcgaggaaa aagactcgcg ctttgtcgtc tggaaacgtt
     8701 tcgcgaagga cgtggacgga atcggctacc acccgttcta caatccgaac cccggttggc
     8761 ttctggacta ttcggcagac atccgcgcct tccgcgcctg gctggcgcgc cagggctttt
     8821 cgggcacgct ccacgcgagc gaatggaaca tcaacggccg tcccgcgccc atcgatccga
     8881 aggacgcacg ggacgtctgg tgcggcatct acacgcagag cgagatcgcc aaggccaagg
     8941 aggttctgca gcagatggtg cgccatgccg gcttcggcat ccccagttgt ttctgcgaag
     9001 cgtacaacgc cttctacgca caaaccgagt tgagccttct caaaggatcg ttgctgcagg
     9061 atccggtggc tccgctccag ccgacggccg cctattacgc gttgcgcaat gccgccacgc
     9121 agatggacgg ctttgagccg gcggccttca cgcccgatgt cgccggaacc gtgcccggtg
     9181 ccgtggcggc gttcgcgttc gcagacggtg cgcgccgtgg cgtggctgtg tggtacgagg
     9241 acgaccagcg ggcggacacc tatcggcaaa aacccgtcac gctcaccctg cccttcgcgg
     9301 cagccgcgct tcatgcctac gatcccgtca acggtgtgcg ccagcgtctg aatgccaccg
     9361 tggacagcgg caagaccatt gtgtgtgatc tgctcatcgg cgatgcgccg ctgttcgtcg
     9421 cggaatagtc cattggtttt ggctttgctc cggtccggtt ctcggctatg ctgaaagcca
     9481 tgaattacga ggtgataccg gtcggaccgt atgcggcgaa ttgcagtgtg ctgtgggatg
     9541 agccgggaga ggcatgggtg gtggatcccg gtgaggatgg ggatcggatt cttgcgtttc
     9601 tggaaaaacg cggtctccgt gcgggtatgg tggtgctgac ccatgcccat ttcgactaca
     9661 tttccgcgac gaatcgcgtg ttggcggttt atccggtgaa ggtttatctg caccagaacg
     9721 atgtggccat ggcgttttca ccgttgaatg cgatgccgcc ttatcgggcg acctcgcgtc
     9781 ccgccacgct ggattcatcc aaagaagacg gcgagactct gacctgcggc ggactgaccg
     9841 cacggcttat ccatacgccc gggcacacgc ccggcggatg gtgtctgtat ttcgagcaag
     9901 aaggtctgct ggtggcaggg gacacgttgt tcgccggatc ggtcggacga actgattttc
     9961 cgggaggcag tatgctggcg ctacaacgtt cgctggatcg gctgaagaga ctgccggacg
    10021 cgacgcgggt gatctgcggt catggcccga tgaccacgat cggcgcggaa aaacggggca
    10081 acccttattt gaggtagtgc gtcgggaacg ggaggcgggg agaacgatgc gcgcgacgga
    10141 tgagattctg acggccttac ggcaggccgc tggcggcgag gtgtcgagcc gggcattatg
    10201 cgaccggctg ggcgtgtcgc gggccgcgat ctggaaacgg atcgaggcgc tgcgcggact
    10261 cggatacggg atcagcgcgt cgccacggcg cggttatcgg ctggagagtg tgccggacct
    10321 gccgcacgcc gccgaggtgc ttcccctttt gtcgacgcgg cggatcggcc gggattgccg
    10381 atatgtcgcg gagaccgggt ccaccaatcg cgatgcggcg gtgctggccc aagccggcgc
    10441 cgaggagggg ttggtcgtga cggccggatc gcaacacgcg ggccgcggcc ggatgacgcg
    10501 tgtgtggttc tcgcccgcag gggtgaacct ctatttttct ctggtgctgc ggcctgcggt
    10561 cgagccgttg cgcgccgcgt ccctgccgct ggttgcggga ttggcggtgg cggaggcggt
    10621 ggcggatctc gcgcccgaac tccaacccgc ggtcaagtgg ccgaatgaca ttctggtggc
    10681 cggacgtaag ttgtgcggca tcctctgtga gatgcaggcg gaaatcgatt gtgtgcggca
    10741 catcgtcttg ggggttggtg tgaacgtcaa tctggaacgt gccagcctgc ctgaggagat
    10801 ccggtcccgc gcgacctcgc tacgcatggc gtgcggcagg tcgttttccc gcgcggcggt
    10861 gctggccgcg atcctcaacc atttcgaacc gctttacgat cagtggctgc ggaaggggtt
    10921 ggagccgttg atcacgcgac tggatgcccg cgacgccttg cgcggccgca tgatcacgct
    10981 ggagtcgggc ggcggccaga cgctgcgcgg tcgggcggac ggtattcagg cggacggggc
    11041 gttgcgtctg gtgacggagc gtggaccggt gaccttgtac tcgggtgaaa cgcatatcgg
    11101 gacctaatca tggattatcg ggccaaacag gataagaccg ggcgtgtgcg gagaatccgg
    11161 cgttgggtga acggtctcgg ccgcagccgt caatgctgtg tttgcggacg gactttcttc
    11221 cggttctcca cctatcgagg gggatggcaa caattttccg cctatctgca caacgtcaaa
    11281 tggatcggaa gcgacttcga tcacttctgg tgcccgttct gccggagtca tgaccgtgaa
    11341 cgtcacctgt tcctgtacgg agagaagttg ggcttttggg agcagatgcg cggaacgtcg
    11401 attttacata tcgcgccgga aaagcacgtg tcccgacgca tcgaggcctg caagcccgaa
    11461 cgttatgtga aaggggatct cgtgccctca cgggaggggg tcgagcgcct cgacgtttct
    11521 gcgatcccgt atgcggccgc atcgttcgac tgggtgttgt gcaatcatgt gttggaacat
    11581 gtcccggatg acgtgcgggc gttgtcggaa ctgttccgtg tgctcaaacc cggcgggcat
    11641 gcgatcctgc agacgcccta cgcatcgggt ttggaacgga cacttgaaaa tcaggcggag
    11701 atcaacaccg atgcccaacg tctggcattc tacggacagg aagatcatgt gcgcctttac
    11761 ggacgggacc tctttgaccg cattcgggga ccgggctttt cgctggaact gattcggcat
    11821 gcggattgtc tcggggatgt ggatgccgcg cgttatgggg tcaacccgga cgagcctctt
    11881 tttttgtgcg tgaagcccct tccggctgtg tgatatgctg aggtcatgaa tgaggtgtct
    11941 atgcgtgtgc ggattcgatg ggtctctttc tgggtgacgc tggcgttttg cggtttgatg
    12001 ggggtgtcgg ctgaagccaa atccaaagag gagccgcaga aagtttcggt gttgatgaag
    12061 gaggcgaatg acctgatgtc gcaggcacag tccgcttacg tcgacggtga tgccccaaag
    12121 gcgattgagt tgtaccgtaa ggcgctggct gagatcgttc gcgtggagcg tgaaaacccg
    12181 aaccgggtca cctccgccga gttcgcgccg gttcgtttcc gcaaggcgtt gtgcgaaacc
    12241 gagatcgacc gtatcctgat cgaggagatc aatgcgtcct cacggaccgt cgcggtctcc
    12301 gacacccgtg cattggagga gaagcggaaa gcccggcaag aggcggcagc caccaaccgg
    12361 gttccggaag ccaccataaa actgtcggtc aaacggggtg tgggaggggg tgacgcggtt
    12421 gatcatcccg aagacaaact tgcggatgcg gtgcggtccg ggtcggagcc ggttgctgca
    12481 aaaaccgacg cgccggtgac caagtccgag accggagcaa ccgcgaagct ggatgtgaaa
    12541 aaagaactct cctgggtacg ggaactgttg tcgcttgaga agtgggatga ggcggatcaa
    12601 aacctgcgca cggttttgcg gcatgaaccg actagccggg atgcgcgttt tctgatggcc
    12661 ctggtacaga tgcgccgagg cagggcggac gatgcgtcgg tcatgatgga cgatctgctg
    12721 gcagatccgg tgactgacga atcggtgtgt ctgctggcct cggcggtata tgccgccacc
    12781 ggatcgtacg gcaaagcaat ggccgcgttg gatcaggcca tgcggctcaa tcctcaacgg
    12841 cctttaggtt atctcaacat ggcttggctg ttgctcgaaa tgcggcctgc ggatctggcg
    12901 gagccggaga tgtattaccg gcaggccgtg aaactcggcg gcgcgcgtga ccgtgacatc
    12961 gagcgacggc tgggaatcaa atccgagtaa tgcctgagtg atgccgggcg caacgcgacc
    13021 ggccggatgg gggacatgga cgcacgtctg acaaaaccgg aaccggcccg ccgctggagg
    13081 cggttggctt cggtggtgtg gtgcggtgtg gccgtagtcc tgtccggact tttgatcgtg
    13141 gacatttggc tgttccgggt tggaatcgtg acgcacatgg cgcgtatgac actggaccgg
    13201 tacggattct cggatacaga gtttgcgatc gccgggttcg acacgcgggg tgtggtgctg
    13261 gaagatatcc ggatcggtca gccgcagccg ctgctggagg tcgatcagat gcggcttcgg
    13321 tttcgcgtgg gcgatctgtt gcgcggcagg ttggagcgtc tggatgtcga gggcgtccgt
    13381 ctcgacatgc gtctggacga acaaagaatc acgctgccgc tggcggaact tctcctgccg
    13441 cgattggcaa cgatccgcag gccggaccgc aagtccgcgc ggatttttca actcgacgcc
    13501 gcgaccgtgc gcgagatgcg gatcgacctg ctgaaccgca aagaggtgcc cctgacgcaa
    13561 ctcgttttag aactcagtgc ctttgctgaa ccacatgggt gcacgcgcgt ctggggctcg
    13621 ctcggggatg ccgcaaccaa cttgtgtgta cgtgtggacg gtacgctcga accgggaacc
    13681 ggaaacctgt cgctgcgctc cgatatgcgt gttgcgacac tggcgccggt cgcggactgg
    13741 atcatgcagg ccacggccgg cgaagggagg aggcaccgga ttccggtacg tatcgaaggc
    13801 ggcttgaccg cacggggcga agtgacggcg gaacgctggc gtgaaatgcg gacagggaac
    13861 atggaggtcg cgctggaccg gcagaccgtg atccgtttcg agggggagga tcgtttcgtc
    13921 cggtttcaga atctgcgggc tgatctcacc ggtgcgccgg atgagtggag tctgcggttg
    13981 catgccggcg tgtcgggttt ccggttggat gcggagcgtg ctgtcgcgcg ggagagcggt
    14041 cggctgctca gcctcagggg gaacgcgaga cttcagcgga cagggaggcg gcagcacgtc
    14101 acggccacgc tggagtcaga tcttcccggt cgttcgctgg gacagctatt ctccgcgatg
    14161 ctgccgctgg ttccccgatt gtttgccgaa ggcgggacac tgcgcgtcga gaccgatctc
    14221 acagcggagc aaggtgggaa atggacggga cagaccgagt ttctggctac ggcaaagcac
    14281 tcgtccgtca gagtgcccgc cggtcgtgcg ggtgccgaat ccgtttcggt ttctgctacc
    14341 gtaccgatgg aaaaaggaaa agccggcgca ttgcaggcgc gtgtggcggt ggaagacggg
    14401 tttgttttca aaaaagggtt cgtgttgcgg ggcggggcag agatgcggct gaccgctgcg
    14461 ccaccgtatg atacagcgaa cggcaccctg accggacgca ttgccgagat gcgggccctg
    14521 cggaaacgcg gcgtcagcat gtcgcaagaa ggggtcgggt tcgagagtgc ggttcaggtg
    14581 agcgggctgg taacgaatcc ggtgtggcat ctggatttac atatgccgga aactgcggtg
    14641 gctgttacaa accctgtttg ccacgcctct gcgatgatgg gcgcgcaagc gcgcgtgcag
    14701 tacggcgcac aagttctcgc ggcggaaggc ggagtgtggg tacgggacgg ggcagccagg
    14761 ctcactgtct ccggtggggt tcagaacgtg gtggccgggc tcgaacgggc tgcgttacgc
    14821 tttgatttgc cagcgtatgc ggtttccgca ccggatcagg cggtcgccga cgtgtcggtc
    14881 gaggtcacga acggatggct ccgcgcagat gaccggttgg cgcttgaagg gcttcagatg
    14941 ttcgtcccgt tacgagggtc agtacgggac ggcataacct gtctggcggg ccagcgcttc
    15001 gggtgggacc actgggaagc cgaaggggtg cggctggtgg ccgacgggtt cggtgttgaa
    15061 gcggacacgg ggcagatcgc tggcaggctg ggtgtccgcg cggtcgatag tgcggcacgt
    15121 gtcgacatcg gtgtggttgt gccgatcgcc agaccgggac aggccaaggc gaccgttacg
    15181 tttccggagg cttcgcttga gcgtgacgat gcgctggccg cccgcctgcg gaaagccgta
    15241 cccgatctgg aggcgagtgc gcgtgtcgcg gcggaggcgg cctttcgtct gggcggcggc
    15301 acaccgcatg ccgtcgggcg cgtgtgtgtc cgggacggcc agtttcgtaa aaaggaaatg
    15361 tcggtgagcg gactggcgtt ggacatgcct tttgaatttt ccccgacttt ccgcacaata
    15421 gaacggccgt ccctcacgtt taaacagatt caggcaggaa atatccggct cgatgcgggg
    15481 cggatcgaat ttgatctcac accgcgtgaa gtgagcgtgg gcagcatgga ggtggggtgg
    15541 tgcaaaggct ccctccgtgc ctattcggtg cttttcgatt tgaaggatcg gaaagatgag
    15601 ttcatcgtgt atgcggaccg gatcgatttg ggggaagccc tgatgatggt gatgccgttt
    15661 caaggcgtga tggagggggt gctgtacggc cggttccccg tggggattga cggaaaccgg
    15721 attcgtcttt caaccgggta tctttactcc ctgccgggtc agggcggacg gttgaaactg
    15781 gatgatgccg ctcccatggc gtcactgctc gatcgcgcgg gcatcaaggg cgatgtgcag
    15841 cggccgctct ccaaggcgtt gagcgatctc ctgttccacg ccatccggtt ggagttggag
    15901 cctaagccgg agggcgacgc ggtgttgcgg attctgttgg aaggcaagtc gaatgacaag
    15961 gactggcccg cgccggtttc attgaatctc aacttgcacg ggccgatcga gaagttggtg
    16021 aatgtgggac tggacttgtc ccgggagtga aagaaggagg acagaatgag acccaaagaa
    16081 cggcgttttc tatccgtgct gctgctggtc gtgacgaccg gctgcatcac cggatgcttt
    16141 tgggtgaaaa ccgaacatga ggtcaaaccg attcacatca cgatggatgt gaatctgaag
    16201 gtacagaaag agttggaaga tttcctctgt ctggactgaa cgaaagaggg aggcggatat
    16261 gcgtaacaaa tggagagtga gaggattgat gctggcctgc gtactcggtt gcgtctgtgc
    16321 ggcgcaggcg gatacgatgc aggagatcaa agcgcggcgc agagcgcgtc gtgagcaaat
    16381 ccggcaactg gttcagaccg gtcatgccaa ggagggggac gaaggatatc ttgtggctca
    16441 gagagcgctg acagcagacc agacggcttt gatgcaagct gaaaacgagg atcgtaaagc
    16501 tggctatacc gcgatcgccc aagcgaacgg caagtccgtc gcggaagtgg ggcgtcaggc
    16561 cgcggtcatc caaaaaaacc ggcgtgcctc aaaacaatga cggggatgag gcaataagaa
    16621 aaggaaaacg aaaaaaagga aagtcggaaa atgagaacac ggtttcgctg ggtttggatg
    16681 acggtcgtgc tgtcagccgg gatggttttc ggcaggatgt tccgggacgg ggaggtcgtc
    16741 tgttttttag gggacagtat cacgaacggg gggcgttacc agaacaccgt ctgggactat
    16801 tatctgacgc gttttcccgg ttggacgatc cggtttgtgc acgcggggcg ttcgggagac
    16861 tctgcgggcg gctcgcttgg acggcttcag gaggatgtga tggattacaa accgacctcc
    16921 gtggccatca tgatgggcat gaacgatgtg ggccggggga attatgtcgc gaatccggac
    16981 gaaaaacgca aggccgcgca acagcgctcg ctggacggat acaaggccaa catggaaaag
    17041 gtcaccgagc gaatcctcaa cgaagcgaac cggccccatc tgtttctgat cacgccctcg
    17101 ccttttgacc agacggttgt gctggagcgg gataacaacc aaccgggatg caatgacgga
    17161 ttgggccgtt gtgcggcgat cgttcgggaa ctggcgtcca aagtgaatgc gacggtggtt
    17221 gattttcacg gcccgatgac ggcgttcaat ctcgaacaac agaaaaaaga tccgaaatac
    17281 acgatcgtgg gagcggatcg cgtgcatccc ggtgcgccgg ggcatctgat gatggcgtgg
    17341 ttgtttttga aggcccaggg ggtgccgtcg cttgtttcgc gagtcgcggt tgacgcatcg
    17401 gcgggacagg ttaccgcagg tgagaacgct acggtgacag cggtcgcgaa gcgggatggc
    17461 ggcgtgacgt tcaccgtgtt ggccaaggcc ctgccgttcc cgacggatcc tgcggcatgc
    17521 aaggcgttgg aaggtctgtt gccgatcttg gaagaactca gccaagagac gcttcacgtg
    17581 accggtctgg cggcgggcga gtacgcgctc gtgatcgacg gcaaggaggt cggccggtat
    17641 tcggcggcgg cgctggcgac cgggatttcg ttgggggcga acacgatgac cccgcagtat
    17701 cagcaggcgc tgaatgtgtc gcgcttgaac gcgcgccgcc gggatgccga gtgtcaggcg
    17761 cgtaacttgc tgaacacccg gcggtggatg aaaaaccacc acaagatcaa tgtggatgat
    17821 ccggctgctg tgcaggcgca ctatgacggg atgaaggata aacgccagta cagcgcggtc
    17881 atggctctca actatatcaa aaaatggccg caatacgagg cctaccgcaa aaatgctgcg
    17941 gaacttgaaa aggagatgct cgccagtcgg actcctgcgc cgcacgtcta tgaggtgcgg
    18001 ccagtcggcc ggtgacacgt tgccttttga ggggttcggg tttgagacca tcacgtcact
    18061 cctgaaatca gtaacccgaa accctcaatg gtcagagaat cagggatgtg ctcgcgttgc
    18121 cgcgccgccg gggaaacgga attcacgctt gccagtgtac gaaccgattt gataaagttt
    18181 agccactttt ttgtgcgccc atcgtctatc ggttaggaca caaggttttc atccttggaa
    18241 gcggagttcg actctccgtg ggcgcgccat cattactata tctcgaaccc tgtgcgccgg
    18301 attgagaacg cttgagtcgg gcggcggttg gccttctaca atttcatgtt ggggcccggc
    18361 ttggtccgga atgtgcataa cgggttcaaa tgattgtcat atgcgcgtgg atatcttatt
    18421 ctttttccca ttcccatgtt ggggtgaccc atgcgtaaag tgaaaatcag acgaatcgaa
    18481 cggatcgagc attatctggt ccgtctgatt caacaacccg gtgacgacca aggccacccg
    18541 tggccgatcc gcaccttgct ggcattcctg aagggcctgt ctgaggtgtt caaaatcatc
    18601 gtctcgttgc gcctctttct gtacgaggtg ggaatctgcc gccgttatcc cttgggatgc
    18661 caagtcatca gcgtcgggaa catcacggcg ggggggaccg gcaagacgcc ggtggtggaa
    18721 attttcgcgc gcgagttgca gcgctcggga cggaaggtgg cggtactcag ccgcgggtac
    18781 cgtaagaaag agttgccgtg gtatcagcgt ctgttcaggg aacagatcga accgccccgt
    18841 gtggtcagcg acggcaagcg gatcttgctc gacagcgaga tgggcgggga tgagccgtac
    18901 atgttggcga gtaacctgcc cggtgtggtg gtgcttgtgg accggaatcg cgtcaaaagc
    18961 ggacggtatg ccgtcaaacg attcgggtgt gacacgctga ttctggatga cggcttccaa
    19021 tatcagaaac tcaaacaccg tctggaggtg gtgttggtgg acaagaccaa cccgttcggt
    19081 aacagccagc tcttgccccg cggtgtgttg cgtgagccgg tccggaacct ccagcgcgcg
    19141 gatttcatct tcatcaccaa gtcggacggg gattccgatg cgttgcgtgc gcgtctccac
    19201 gaattgaatc ctacagcgga aatcattgag tgccgtcatc gggcgcggta tctgcagcat
    19261 gtcttcactg cggagcgtct ggatctcgca tggctgaagg gccgtacggt gacctcgctg
    19321 tccggtatcg cggtgccgca aagtttcgaa aattcgctac ggaccatggg cgcgcgcgtg
    19381 atccactgcg aacggtatgc ggatcaccac cggtatcacg ctcaggagat cattaatgcg
    19441 gtcaaccggg ctgcggatct caaggccgac gccttgctga ccacggagaa ggatgcggtc
    19501 cgcttccccc gtctggaggt cacgccggtt ccggtctact acctgcgtgt ggacatcgaa
    19561 ctgatgcgtg gtgccgaaaa ctttcacgag gcggtggagc gtatctgttt tcggcctccc
    19621 ccccgcgcat ctgccgcgcg cgcgcagtcc gtgccggtcg cggaaaagac atgaggagcc
    19681 ggtgttgagt gtatcgcaga cagagcagaa aggcccgcat ccgcgcgtat tgcgttcggt
    19741 ttccgtggtc agcctgatga cagctgtcag ccgggtgctt gggttggtgc gcgaggtgac
    19801 gatggcctat tttttcggca cctccgcgtt gaaatccgcg tttgacatcg cgttcatcgt
    19861 gccgaatctg tttcggcgac tgttcgggga gggagcgctt tcgagtgcct ttgtgccggt
    19921 gtttggcgag accctgatcc gggaagggca cgcctccgct ttccggttcg ggatgcgggt
    19981 gatcagtatg ctgaccgtcg tgctgggatt catcacgacc gcagggatcc tgctctcctt
    20041 cccgctcgcg cggatgcttc cgccggaaag ccgttggctg attccgttgc cgatgctgcg
    20101 cgtcatgttg ccgtatgcgt tgctgatctg cattgcggcc ttactttccg gcatgctcaa
    20161 cacattgggg aagtttgcga tcgcgtcgtt gacccccttt ttgctgaacc tgatctggat
    20221 cgtgacgttg ctggtggttt gcccttttgt tttccgtgct ccggaacagc agatccaaac
    20281 gctcagttgg gcgattctgt tggcgggact ggcccagatc cttttccagt tgccggcgtt
    20341 gggccggcac ggattccatt ttcgtctgcg gttccgcggg atgttccacg atccaggagt
    20401 gaaacgcgtg ctgtatctga tggggccggc ctcgctcggg atcgggttga tccagatcaa
    20461 cgtgtgtatc gataaggttc tcgcgttctg ggcggacccg gccgcgccag cggcgctcga
    20521 atatgccgag cgcctcattt atctgccgtt gggggtgttc gggaccgctt tcatgaccgt
    20581 actgctgccg actttttcac gtcaggcctc tgcgggcgag ttgccagcga tgcaggcgac
    20641 gctggagcgt tcgctacgga atttgtcggt gatcatggcc ccctgttcgg cggcgttgtt
    20701 cgttctggcg ctgccggtga tccttttggt ttattcgttc cggggcggac gttttgatga
    20761 gacttccgcg ttgttgagtg cgcgcgcgtt ggcagcgtat gcgccggggc tgctggtgtt
    20821 cagttttcaa aaagcgatca cgcccgcttt ttacgggtta caggacttgc gcacgccggt
    20881 gcgtgtgagt ctgtgcgcgc tggtgctgaa tatcacgctc aatgtgttca gcgtgctgtg
    20941 gttgccgcac ggctggaaac atgtcggaat cgcgggctcg acggtgctca cgagcttggt
    21001 gaatgcggtt gcgctggtga ttctgcttcg gcgccgcacg gtggccccgc gcagggaggc
    21061 cgtgataccc acgctgctga aggcggtcgg ttgttcactg gtgatggctg tgggggtatg
    21121 gcgggtttgc acggtaggca tgcgttgggc cgacgggttg ggggagtatg tgaaaggagc
    21181 gcagatcggt gtgatggctc tgacggtcct ctgtggtgtg atcctctatg gggggctgat
    21241 ggtgtgggtg gggagagggg cggtctggga aatggtgggc gagttccggc gacggccggt
    21301 gaagggggta cgtcaatgaa gccaaaaacc tttgtggtgg cgtgcggcgg aaccggggga
    21361 catgtgtttc cgggtttggc cgtggctcag gagttgcggt tgcgcggtca tgcggtggac
    21421 gtctggttct cgggacgggc agtcgaacgt tccacgctgt gcgattggga cggccctgtt
    21481 tttctgaccg gtgcgcgcca actttccgtc cggacgctgc cgcagacact gaacgccttc
    21541 tttcggtgtg cgcgtgaact gaaacggcgt cggccggatg ccttgctcgc catgggcagt
    21601 tataccagcc tgccgccggt ggtggcggcg cgactcgcca atgtgccgat cctgttgcat
    21661 gaagccaacg cggtgccggg caaggcggtt gaggtcctgt caggcatggc ggaactcacg
    21721 gcgatcagtt ttgaagagac cgccgcctgg atgcctcgcc gcaagacggt gttcaccggg
    21781 ttgccggttc ggcaggaatt gatcggcaag ccgcgttttg acgagatccc ggaaggaatg
    21841 ttcaccgtgt tggtcacagg cggcagtcag ggcgcgcggc gcgtaaaccg tctggtcaca
    21901 caggcgttct gcctactcca gcagtccggg tccggagagt ttgccgtctt gcaccagagc
    21961 ggggcggccg atgaggctcg ggtgcgccag acatatgagg aagcgggggt ctgggccaga
    22021 gtacgggcgt tcgagccgga aatggggcgg gcttacgctt ctgctgattt ggttgtctgc
    22081 cgggccggag cctccacctg tttcgagtta tgtctgctcg gcaagcccgc gctgatgatt
    22141 cctctgccgt ccgcagtccg gaaccaccag tttctgaatg cctctgcgct cgcccgtagc
    22201 ggcggggtgg atgtggggat cgagaaggaa ctttccgcaa ggtcgattat gcgctatgtg
    22261 cagaataaaa tgcggacgcc cgcatgcctc atgcacatgg gggaagcctt gaaggccttg
    22321 gcccatcccg ctgcggcgca acgggtggcg gatgcgctgg aaagcctctc ggaagcgtga
    22381 gcggcagtgc tcatcagggc tcaggattcg acgtgcgcga agcgggatca gccgacgttt
    22441 actgcgtgcc catcatctgg ctggagaaca tctgaaagaa gagttcctgc agcacagcgc
    22501 gcccgtcgcg attgatgcgc tgcagcgccg ccaattccgc ggtgccgaag cgtaacgaag
    22561 ccgtgaacgc accgttggcg ccgcgcccca gacccaatgc cgcaccgtcc cccccggtgg
    22621 gaaacgggcg gagatgctcc ggtttcacat tcgggataat gctcaccaga tgacgcatca
    22681 gactcgccag gcgtaaattc gcgccggagg tcagcggtgt gtccgccagt gccggatcct
    22741 gcatgcgcaa ccgtcgttcc agcgtgagtt gccgttcccg aaacgtcaac tccgtcagtt
    22801 ccccgtccag cgtttggacg gggccaaccg ttgtaatcac aaacccttcc gtgacggcgg
    22861 tttccaatac ggcctgtttt agaaagagtt cgagcaacgt gttcaggatc gagacgtcgg
    22921 tcttgccttc cgcttcggct tcgggcgcgt gaaacgcgag acgataggat acgatctcca
    22981 caccggttgc ggcacgccgc gcgggaagcg gtttgagggt gatgccgtca gcatggctca
    23041 cggtgtgcag tgaacggatg gctttctccg cgtctgcggt ctgcacgacc ggctcgatct
    23101 gcacgaggca gatgccacgt tgatcccgag tgggggaaag ggagatcagg caatcgccgc
    23161 tgaacccgtt tgtgggcgcg attcccgcat agggtcgtac cagactgagc cgttgcgaac
    23221 cgagatacgg atcccagagc tgcggcgcgt cacacgctga tacggaggcg aaaaacgcgc
    23281 gatccggcac gccgtgccag agtgtcgcgc gcggcggacg catcgcggcg acgaggcggg
    23341 caagtccggt gccagccgtt ggtcggccac gcacagtcag ggtgagggcc tgactttcga
    23401 aggtcaatgc gaaagagaag gtctcgcatt cgcgaaggaa acggtccgtg ttgacgaacg
    23461 ctccggcttt gtcactgcga actcctgtca ggtcagccag ccgttgcgga ttgaccagaa
    23521 cacgcagggt gccggggatc gactgggggg gggcgtccaa cagaagcgaa cggttttccc
    23581 aagcccacaa cacggcctca gccgaagacg aggtgaacag gtgccgtttg acgacaacca
    23641 ccgtgacgac cggcgtcagg ttagtggagg cgggctcttc aaaccgggtg aagggttcga
    23701 cacgcgtttg tttccggtag gctgcggcca atgcgtcaag cactgtccgc ccgtcgtccg
    23761 tcaacgggat gattgccaca ttggctggat ttcccgcacc aacgggcttt tgggtctcta
    23821 ccgtttgcac gatacgcagc gggcgattcc gatccacacc cgtgaacgac ggcaggcagg
    23881 ttgcacggtc cagcgcacct tgaacccact ctaacgccag cggcaaatcc gcgaggcggc
    23941 tgaacgccga agcgccgtag atcacgcttt ggtaactgtc cgtttcgaga ataccgacaa
    24001 agagacgttc agcccggctg acgcagggca gaagaacggc gagggacaac cccgccatcc
    24061 gcagagcgga ggggaaccgt acaatgaact tagcacgttt cgtgttcatg gtttacgtgc
    24121 ccggggtttg gggtgatgga acatcatcac cagcagccgg cattgttctg caagatctgg
    24181 atcaccgatc ggatccacat agctgaccgt tttgttttgg atgttggccg agacgtaaat
    24241 ctgatccgcc tcctcgatgt ttttcggttt gaccggtttt tcaaagagtc ccttgtccac
    24301 caaagtctgg aacaggaata cggcttcgtg tcgcggaatg gtcagtcgcg tctcgcggat
    24361 atcgtaccat tgctggtggg tcatgtcctt ggcgaacgga ttgctgacca gcggactgct
    24421 tccttcgcgg acagtgacga tcccgttccc gtccagacgc acccagacgc gttgggtcgg
    24481 attgccgatc cggtaatggc ggatatccac ccagttcagt cctgattcgg ttactgtaaa
    24541 ataggggttt tccatcgggt gttggcaacc gcacacgata accagtgcgg ccgcagccca
    24601 ggcatgtcgt ctcatggcat cacctttttt tcacgtccat cacataaacc tgacgggaac
    24661 cctcgtgcac agagttgaac gcgagcatgc cgccgtctgg ccgccagcgc ggatgcaggt
    24721 cacaccgcca ataggtgccg cggtaggatt ccggcacgaa gaatccaccc aacggcagaa
    24781 tggcgtgatc cgccatacgc aagaccatga gtttacgctc gttcaggcgg tcggggtagg
    24841 tgtccgtcgc catccaaagg ccgtcatccg aaaagacccc gtgaccatcg aaatccagca
    24901 tgccgcggcc gacgcgtgtg tagtcggtct tgccgaccgt gaagagcaca tgcgtgtata
    24961 cttttttctg atagttcgcg gtgaccatca gccggtcgcc gtccagccaa ttgaaatgcg
    25021 acccgcccca gccgtccgga aaacaacgac gcacgccact gccgtcacga ttgcaggtga
    25081 aggacgtggt tttccagttg cgtgcggtct tggggttgac cgaacgcgcc agccaaaaga
    25141 ttttggatcc gtcccgactg aagaccgtat ggcagaaata ggcgaggtca accccttctt
    25201 ccacggaggg catctgttcg cggactgatg cgatcggcac gatcagcttc gccgtacccg
    25261 tctccaggtc gaccagaaaa agcccgtcgt cctgagggaa agagacggtt tcacgcgcgt
    25321 cctgtccctt gccgccgtat ccgtaatcgg ggcgcgtgag gcgtagccgc gcgtaattga
    25381 tgctgacggc ctctttaccg gatggggaga ccgcgctgac ggggaacggc acgatccgtc
    25441 gggtgcgggt ctgcaaatca aagatgaccg tcacgaaccg gccgtcgacc agatcattga
    25501 aaaggaagtg gcgatcgggt gcggttgcca gccagtgagc catggccgcc tcctgaaagt
    25561 tccagcagcg tgtggtggtc aagggtatga agcggttgtt atcctgcagg tccaccacac
    25621 cgagttccgc cacgtccgct tcggtcggga gacggttttt cacatcggtt tcgagcacgg
    25681 tgacgtagcg gttgttggcg ctccacgcat taatcgcgta atacgaggcg aacaggtggt
    25741 ctttgggccc cgacgtgatg gctcgcgcgg cggagaaaac cgggaacggt tccgcattcg
    25801 cgtgagcggt gagcaacgag atcaggaggg aaatggacag gctatggcgt gtcagatgca
    25861 tggtccgatc ctttcatttt gattgcgtgt gtcgtgccgc acgtcttttt cccgtgtttt
    25921 gcggtaagcg ggtaaggacg acagcatgtg gcgttccctg ttacagtctc aggaaacagg
    25981 acatccaaaa aacattcggc gacttgggcg ccttcgggcg gggagatcgc catgtccggg
    26041 ctgacttcga ccacggtggc ccggcgtgtg gcgcggtcga tcagaaaatc tacgccgatc
    26101 agcggcaccg gcagagcacg gacgatgcgc tgcgccagac gcagtgcgta cgcgggcacg
    26161 gcgtgcgtgg cgagctcgac cgtacctcca gtgtgataat tatgagtcag tcggacttgc
    26221 accgcacatc cggcagcggg cacgtcatcg ggcgcatgtc cctgttccgc gagattgtgt
    26281 cgggtttccg gatcccacgg gatgaggttg ctagggtctt cggcccggcg tcggcggttg
    26341 tgaagcgtaa tgagttggcg gatcgtgtgg cgtccgtcgc ccgtgacctg cgccggcttg
    26401 cgccggatcg cggcggccag cttgccaccc gccagaatca cgcgcagatc ttcacccgga
    26461 acggtttcct caagcaacac atcgcgttca tagcggcgtg cgcgacggat ggccgcatgc
    26521 agttcagcag gggtgcgtac ccccatagca acaccgcgcc cgccccattg gctgtcaggt
    26581 ttgaccacca ccggcgcgtg tgtgcgcaga aaggcgagcg cttcttcggg ttgcgcgtcc
    26641 cggtaaacgc attgcgtcgg caccgggatc cggtgacggg aaagccagcg atggcaggcg
    26701 cgcttgctgt tgagcaagtg gtaggtggcg gctccgacat gatccgtgag cgcgttgaaa
    26761 caccgtacgc tccggccctc ccggctgagg gtgaaaatcg gcagttcggg gtcggggtta
    26821 atcatcgtga cgcggatgcc gcggcgtcgg gcggcatcgg tcaatgtagt cgtatacggg
    26881 gtgagggggg gtgtattcat cgtttcaacc ggttctcgat cagcaccggg ccgccccact
    26941 tgccggtcgt gaccagatcc caatcgccat tgccgtccat gtcggcgacc acgataccca
    27001 gtttgccgtc accatggaaa tccgcgatgc cgcacgcttc agttcggttg gttctgatcc
    27061 ggtgggggat gaacgtcact tcaacggcgg cagtaccgaa cgcaagaacc ggaaccacgg
    27121 acagcaacag aaaacgggac atgcgcatga actctctcct gatgcgaaac agtatagcgc
    27181 ggcatgcggg agtttgtctt gcaaaaaaag attgggcagg gcaatcggat cgattttcac
    27241 cattgacatc aatgccgata aagcgcatgg tctttcatag caatagggca ggtgtttcgc
    27301 ggacaaaaac ctagtggttt gcaaccccct cgaataggta gtaaaaaatg aaaagaaaac
    27361 ggatgatggt tgtcggtgcg gttgtgttcg gcgtcgctgc ggtgcgggcg gcagacgttt
    27421 attggtggaa gggttcagcc ggggatcata agtggtcgac ggcggacaac tgggcggttg
    27481 gctcacttga aggcgatacc gcgaccgagg cgccgaccaa cggtcaggac gtgtggcttg
    27541 tcgggtatgc gggtgaaacg atcatcaacg acgttgacgg tgtcacgctt aactccctcc
    27601 acacgaccaa tcaggtcgac accacgcatc cggtgatgct catcggcaaa ccgctgacgc
    27661 tgagcgccac ctacaaatgc tacacgagga caaccaacga ggtcggtctc gtcttcacca
    27721 cgaccagcgc ggtttcgatc tatggcgcca acaattacaa cgtcatccgt ggcggcatct
    27781 ccgcagagaa cgcgatttcg ttgactttta gcaatgcgcg cgatctcggc tacgactgtc
    27841 cgatcttcaa cgtctatggt gcggtgaacg tgccgaaggg gagtgtgcgg gcggcgcagg
    27901 gcaacagcgc gacgagcttc tacggaccgg tgaccgcagt cacgctttat tcgggtcagt
    27961 actatggtaa cggacacgtc catctctacg cgatcggcaa caatatcggc accgtctacg
    28021 catccaagta caacatctgc aatttcaacg ccgcaaactg cttcgtgaac aatcccctgc
    28081 tgtgctggga ctggaacaac aatacggata gcaatggcga gaccttccgg ttttatggcg
    28141 atttgacgat cgatcatttc gagagcacca cgcttcagtc cgccgaggtg tcgaacgtga
    28201 agcgggcgat cggcgctgcg agcgggatct cgaccgtgac gatgaaggcg acgaccaaca
    28261 gcaacacctc ggcgagctac aacgacaaac tgtcgctcgt ctgggatccc cagggcgact
    28321 acacgttcat gaacaccaac cgcatcagca cgatgaccgg tacgatcacg gtaaagcgcg
    28381 gccgaatggt ggtggcgggt acggcgaatt tcactaaact caccggtatc acggtcgagg
    28441 acggcgcggc gttcgaagcg gcgtcgactt cggcggcaac gactttcccg ctgctgacaa
    28501 cgcttacgct cggccgaaac gcgcgcttcg acgtgaccaa cacgacttcg accgtcttcg
    28561 cccaagacgt cactttgttg gtgctcgccg aaggagcgaa gctttacatc ggcagggaca
    28621 tcgagttgaa gttcacgggg gtgaatcgtg acggcgtata tcttgccagt acgaattttt
    28681 cggcggcaac ctgcgactgg atcgagggtg agggcacagt gacgacgccg gccgtatcga
    28741 cgttgacgag ttggaaaacg ccagcggacg gcgattggtc aaccgaggcg aactggtctc
    28801 atggcgtgcc ggcggcgggc aaggagacgt atctcaccat acagggcggt acgtacaccg
    28861 cgcgggtgga cgtcgcgtcc ctcgtgaatg ggcgtaccac gcttggaaac acggacggaa
    28921 atgagtcggc gctgctggtc gggaacgatc tcgcaatcgc ggacggcaac tggctccaga
    28981 tcaacgccgg cggtgcctac gtgcagacga atggcgtgag cgagttccgt aatactgcgg
    29041 aggcgcgcag gacgacggtg aacgaaggtg gcctgtggcg gctcgaggga acggcgacca
    29101 accgattcct caccagtgac agtggcttct tcacgcttgc gggcggcagg atcgaaatcg
    29161 ccgatcaagc ggagctgtat ttcgaaaaca ttcagcatgc ctacaatcag tattcggctt
    29221 tggacgcggg taggatcgac atctccggca atggcgttct caagtacagt tcggcgcaga
    29281 attcgatggc gaataatatc cgcgatgccg aaatcatcgt aagggataac gcgaagttcg
    29341 ttttcaccta tgcgtcgttt gcgtccaagg cgaatggcga attccgtctg gtcgtcacca
    29401 acaacgcctc ctacagggcg agtgcgtgca cctacgtcgg ttacaacggc tattccgcgg
    29461 tgccctccca ttgtcggcag atcttcgatt tccacaccga cgtttcgccg agcaactact
    29521 tcggctggac gggttttgtc ggcgtcaacg cttccggcta cagcgaggtc aacatccatg
    29581 agggcgcgat ggtgcaactc ggcggcggct acggttgccg gattggcgcc tgttcgggca
    29641 agagcgacaa aagcgtctgc agtcccaccg gtgtcgtcaa cgtggccggc acggcccgtg
    29701 tggaaggcaa ttactattcg acttacatcc agcagaacaa ggctggatac aatgccttct
    29761 acggactgtt ggtcggcggt ggctatcagt tgtattacga ggacatctac aacaacaggg
    29821 tgcaccggga actgccgact gattcgcgga tgaagggtat cgtcaacatc gcgccgacgg
    29881 gatcgttcct tctctggcag ggaatgctcg cggtgggtat cggcaccggc ggcgacggcg
    29941 agatcaacat cgacggcggc accttcacca aggcccgcga cggtaatacg gcgatcggca
    30001 tgggtggcgg aaagggcgcg gtgacgatca ccgggggcgg cagaatggac gcctacggga
    30061 cgctgttcat cggcggcgtg cgcacaaacg acttcgaaag cgtggtctat tacgatgact
    30121 ggtacgtcga cgacggcgct ccggcatccc tgcgtgatgc cgagggtacg ctccgggtgg
    30181 tcaacggcgt gtaccgtcag gctggcggcg cggcgaagat cgtcgttggc gcggagggta
    30241 cggggatgat cgagatcggt tcgaacggcg tcgtgcaggc cgaacatgtg gtgatgtcca
    30301 acggcgtcca gtcggtcctg cgcttcgttg ccggtgcaaa cggcttcggc tatctggatg
    30361 cgcagaaatc cctgtcgatt tgcgacgggc agcgtgtcga ggtggacctc acggaatatg
    30421 ccgggagcaa gacgaagcat cagttggtca agttcagcgg cacgaacgag aatttccgtg
    30481 tcgacgtttt gcaactcgac gccgttttca cggacgagag cggcaagaac cagcataaag
    30541 tggcggttct gcgcaagggc gccgacggtc tgtacttgaa tctcatgcaa ggaacggtac
    30601 tgcatttcaa atgaggaaag tgaggaacct tgacgattac cctttctcgg aaaaagttga
    30661 cccttcttgg caccttggtt ttcgccgtag tgcacttggg cgcggttgaa accgtgatcg
    30721 atgcggcgga gacaccgcag attgagaagg aaaccgttct tgccgtcggc gatgtgctca
    30781 aactcgtcgg taagaaggcc gattttgtcg ccaccgcgcc gatcaggggc gcgggggcga
    30841 cgctctacgg ggcgctcggg tctccgggct cgtgtacaca tcaggcggtc ttcgaagcgc
    30901 cgatcgaatt tgataccgtc ctgtggggac aagactacgg cgaatcgaag ctggtgctga
    30961 aagcatccgg aaaccgctgg cacaaattcg agctcgcgta cgcgaaggtc gaatgcgcag
    31021 cggcgaacgt gctggcgccg gaaggagtgc tcacctgggg agactactac cgcggcaccc
    31081 tcgaccgtaa cgcgaccttt gatctcggcg gtttcgacca gacgatcgat cggctccatt
    31141 cgacgagttc gccgggcacg aagaattccg accatgtcgt atccacgggc ggcacgctcc
    31201 ttatgcgtgg taccgctgat gccgtgtgtt ttgcgcggat cgacggggaa ttgaatctcg
    31261 tttggcatcc tacgggcgat ttcacccaga gttttgacga gcgtgtcaat tcggtgcgag
    31321 gtcggattga ggttcggcgc ggtacggtgc gactcggtaa aagcggggcg tttcggaaca
    31381 tcgacgaaat caaggtggcg gacggcgcga cgttcgttgt cgaggcgacg aatgcacttg
    31441 cgttggcggc ggtgaagctg gtggaactcg gcaaagactc cgcattggtc gtgaagggtg
    31501 ttgatccctt catgccgcag caggtgagcg tggtgaagga cgtcggcgca aagatcgaaa
    31561 gcgcggtgcc acttaaactc gcgcgtgaag ccgcgtacga tgatacgcct ccgcgtacga
    31621 tccgcgacaa gttgcaggtc ttttgggacg actggatcgt cgatccggcg atgacgagtg
    31681 cccggcgtgt gccgcaccat cccgaatacg tgggtccggt catcacgacc gacaagcctt
    31741 gggagggtgc ctccggcagc tacatgtcgg tcgtgaaggt cgatgacgtc taccggatgt
    31801 accggcaggg gatgagcagt cacgagtcgg agccgcacta tcacgggctc aagatctgcc
    31861 tcttcgaatc gaaggaccgc gggctcacct gggagcgcgc gaagatcaac cgcttcccgt
    31921 acaagggcaa tcgcgagaac aacatcgtca tcgatttcag agtcaactac tgggacaatt
    31981 tcatggcgtt cagggacgat aatccggctt gtcccgaggc cgagcgctac aaggcgatcg
    32041 cgctctacag ccacaagtgg agcgaggaat tcgcaaaggc gcatcccgac tacgcgccgc
    32101 tggcgaaata cggcaaggca ctgtggtgct ttgtctcggc ggatggtctc gactggaagc
    32161 cgggctggcc gctcttcaag ttcggcgaca tgggactccg cttcgattcc ctcaacgttg
    32221 cgttctacga cgaggtcaac cgggaatacc gcatgtacat gcgcaacgtg aagccgctcg
    32281 aatacgaacg ttacggcggg acgaacgcgc gctgggtgat ggtgacccgt tcaaaggatt
    32341 tccataactg gtccaagccc gagaacatcg atttcaacga cggacaggag gagtacgaac
    32401 tctacacttc gggggtggac ctctatccgc gcaatcctga gttgaccgtc ggttttccga
    32461 cgcgctatac cgaccgccga cgctggtcga agaacttcga gcggctctgc ggcagaaaca
    32521 agcgcgtcga acgcctcgcg cccgatccgc ggcacggcat tgcgatcacc gataccgttt
    32581 tcatgtggtc gcgggacggc aagaccttca cacgtgaaga ggacgcgttc ttctcgccgg
    32641 gtccggaaca tgccggcaat tggatgtatg gcgacggcta tctgcagcgg ggcttcgtgg
    32701 taagtcccgg acgttacggc agcgacgacg aactttcctt ctacgaagtc gacggcgggt
    32761 ggacgggcag ccgtctcaca attaaccgct atgcgctgcg gatggacggt ttcgtgtcgt
    32821 atcatggacg ctaccgcacc gacaaactcg tcaccaagcg cgttgttttc gatggcaacc
    32881 gcatgctgct caacttcaag acctcggcac ggggacgtgt tttcgtgaaa atcaccgacg
    32941 aggcgggcaa gtcgattgtg accgacgaga tcttcggcga caaagtcgac cgtatcgtcg
    33001 acttcgagga cggcgaggtg tcggcgttcg ccggcaggcc ggtgcggatc gaattcgggc
    33061 tttccgatgc ggacgtctac tcgtttcgtt tcgagaaggc ggcggatgcg ggcgcacggg
    33121 acgcatgccc ttaccgtgga cgtgcaggtg aatacaaaac tgaaatcgag aagacgtgct
    33181 ggtcgaacat gaccgacgag gtgcgtattc cctacttggc ggcgctttcg atggaatacc
    33241 gcgtaaccgc cgaacgcgag acgatgctca aggcggatga actcttcatg gtgctgaagg
    33301 acacccagcg tctcaacgcc acggtgacgg agggacaacc gctcttcggc gaactgctcg
    33361 cggtggtgga ctgccactgg cggatcgccc gagacccttg gcagatccgt tggttctatt
    33421 cgagactcgg ggcgtgcgat catccgcttg cacttgcgtt caacgtgaag tacagccgta
    33481 acgatcaagc aaaggcgaag ctgaaggagc tgcttgccgc aggcagggtc aggcccgaag
    33541 aattgcctgt cacctgggat cggctcaaca acgaagacgg ctatgttcag gagcgcttcg
    33601 ccgaccgtct ggtcaaagac ttcgatctca cttcccaacc tcaggatgcc gcaggcaagg
    33661 cccgttggct gtgggcctac tggaccatgc ggctgttgaa caatggccgc tgatctcatc
    33721 cgcagtcacc gtttgaatgc cgtcgttccc caagtcaaaa attcggcttt ccggcaacag
    33781 gggttgcccc cggctcccgt aatcgggtat agtaaaactt tgttgtgccg gaggggccgg
    33841 tacgggttct catgcgggag cagttatgcc gaatacggtt ttggtgggtg cacagtgggg
    33901 ggatgagggc aaaggtaagg tgattgatgt cctcaccagt caggcggacc tgattgtgcg
    33961 gtatcagggg ggaagcaatg ccggacatac cgtgatcgcg gatggccgga aatatgtgct
    34021 gcacctgatt ccgtccggga ttctgcacac ggacaagcaa tgcgtgatcg gtaacggcgt
    34081 ggtgttggat cctttcgacc tgatccggga gatcgagggc ctgcgggaac aggggtttga
    34141 tccactcggc cggctgtata tcagtgaccg ggcccacatg gtgatgaatt ggcaccgtgc
    34201 gctggatgcg gccgatgaag cacggcgctc gcaggagagg aaaatcggaa ctaccggtcg
    34261 cggcatcggt ccggcctacg gtgccaagat agggcgatca ggcttgcgcg cgggggacat
    34321 gctttcccct tcgttcctgg aaatggtgga ggcgggggtg gcggaagcca acacggtgct
    34381 ggccggatgg ggcgcgccgc tgctggatgg gcgtgagacc cgggtacggt atgaagaggc
    34441 ggtccggacg ttgaccccgt acattgccga caccgtgacg ctcgtgaatg aagcggaccg
    34501 cgcgggcaaa agcattctgc tggaaggagc ccaaggcacg atgctggaca tcgatttcgg
    34561 gacctatccc tttgtcacct cgtccagccc cacggctggc ggagcctgca cgggttccgg
    34621 tatctcgccg cgccgtatcg accgggtggt cggcgtgatc aaatgttaca cgacccgcgt
    34681 gggggccgga ccgttcccca ccgaactgac cggcgaaacc ggcgagcggc tgcggcagaa
    34741 gggaggcgaa ttcggtgcga ccaccggacg tctgcggcgg tgcggctggt ttgatgccgt
    34801 agtgggccgt tacgccgcga tggtcaacgg tgtggacttc tgggccatga ccaagctgga
    34861 tgtgcttgac gaggagccgg tgatccggat ctgtactgcc tatgagcgcg acgacggggt
    34921 ccggtatgaa atcttccctt ccgacctgag cgtcttggaa cgctgcacgc ctgtttatga
    34981 ggagatgccc ggctggctca ctccaaccag ttgctgtacc cgctatgagg atttgcccct
    35041 caaggcccgc gcgtatgtgg agcgcctgat cgcgttgatc ggcgggagac tgggaatcct
    35101 ttcagtggga cccgcgcgcg aagccacgtt gcggctgggg ttgtgaccgc aggccgcagt
    35161 accgcgcaca cggaatgagt gtggacacgc aaaccaacag agtgccgtcg cacatcgcca
    35221 tcatcatgga tggcaacggt cgctgggccc gacaacgtgg caagccgcgt ctggcgggac
    35281 atgaggccgg ctccgaaacg gttcgttgtg tgcttaatta ctgccg
//
LOCUS       DAOSRB010000005        33010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1472_length_33010_cov_7.392110, whole genome shotgun sequence.
ACCESSION   DAOSRB010000005 DAOSRB010000000
VERSION     DAOSRB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 33010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33010
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_33010_cov_7.392110"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..2230
                     /locus_tag="PLZ74_00605"
     CDS             347..2230
                     /locus_tag="PLZ74_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96854.1"
                     /translation="MTRTLRLITICSLLAACAGLLSAAPEAEAYRIRRGIPNSRYFLQ
                     NDTVGNQYLFFIGHSVLAGTGLKNSGLRYSACMTKALKRHFPNASPRETRHMYPGGSW
                     FAQYRCSRGQPVFGEVICSGHLAILDFAAEDRSADLEQVKTSLEGLIRQITLYRATHS
                     TILVYTLTPEMLADYRAGRTPAYIEVCERIADHYGIPSLNLAQVAARAILSGTISAEA
                     FSADGIHPTDAGARLWAEAINAFVDALMADGPVPEKPQRVTLPAPLFPGTDDRGQIIA
                     YEHPEVRTTGSWQPGRESPIKPFRHVLVSREPGTRLTLRFQGSEIGLIDVAAPDGADL
                     EYAVDGGAFRTLAAPKNTDKPVLRALPLARGLTRDAEHEVVIRTASDGTARIGGLLVN
                     GSVKNPHAGMSTLERIDAIYAAMDPINYTPPANRFAHIPKTMAKLRGGGELRMVLLGD
                     SIMGNTSGSSFELLLMRNAPACKIIKIASLRSSTGCTYYQHENRVQQYVLRHNPDLLM
                     IGGISNGGDAEAVRSVIRQVRASKPDTEVLLLTPVFGAMRDEHIRTFTREIDTTTPNF
                     RHRLSKVAEEERCAFFDMTGPWWEYIQKSGKTYGWFMGDAVHANARGCQIIGRLLERW
                     FKE"
     gene            complement(2220..3326)
                     /locus_tag="PLZ74_00610"
     CDS             complement(2220..3326)
                     /locus_tag="PLZ74_00610"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOR96855.1"
                     /translation="MPSLPIFSLSDRSVPPLILAPMAGFTGAPMRALCRAHGAELTYT
                     EMTSAAGLLHSSDKTWHLLETFADEGPVVAHLYGSEPEVLAEAAVRVEATGRFAAIDL
                     NAGCPVRKIAATGAGATLAADPQRVHAILKAMRRAVTLPLTVKTRLGPDPGRVTVFEL
                     LAAAEEAEADAFALHARFTSQGHGGEARLDLLAEVKRRARIPVIGNGGVGSPYTAQRM
                     LQETGVDAVMVARAAIGNPWLFSDIRDALVSGRTPSPVETARGRPRRALETIRSVLEA
                     HLEAECELARRCCEREGRAGSVRDLDAAGVAAFRGHLFRYLHGLKGSSYLRGRLHTLH
                     TLEEVRAAVEACLAREAAYRAGARPSGNSNRVTP"
     gene            3478..4260
                     /locus_tag="PLZ74_00615"
     CDS             3478..4260
                     /locus_tag="PLZ74_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOR96856.1"
                     /translation="MREVLDVLLLSVLQGIAEFLPISSSGHLVLAQSLLGVNTPGMRL
                     EVVLHLGTLVSILVYYRNRLTQLVLGTVRGDRESWRTAGQIALSAMPAVFFYLLCHDK
                     INAFFESPRAVGGFLIFTGVVLYALRWMACGQGRLTAARALLVGLAQALAVLPGVSRS
                     GMTIAAGRMAGIAPDRAAEFSFLMCIPLLIGAALMDGLGITPAAPKADALPVWLLLAG
                     AAVSAGVGYLALSLLVRVLRAGRFWLFGLYCIAAGFVTILLT"
     gene            complement(4273..5148)
                     /locus_tag="PLZ74_00620"
     CDS             complement(4273..5148)
                     /locus_tag="PLZ74_00620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR96857.1"
                     /translation="MSPGLADEYELLDSGDGRKLERFGAYTLARPCSQAVWKPRLSEA
                     EWTAAAHAAFDREDGNRWHNRSGLPAAWEVEIAGIRFRLSGTDFGHLGVFPEQRAQWL
                     WLRNTVRDACAARPGPVRILNLFAYSGGSTLAAALGGAEVCHLDASKGMVTWARDNAR
                     LNRLEAHPIRWITDDAHTFLRREVRRERRYDAVILDPPTFGRGQGNETYKIERDLPET
                     LRLCRSLLAPDPLFVLLSAHTPGYSPLVLENVLGQAVGDLDGKIVSGEMLLTGAATVP
                     STPSGAFARWTRGAQ"
     gene            complement(5177..6325)
                     /locus_tag="PLZ74_00625"
     CDS             complement(5177..6325)
                     /locus_tag="PLZ74_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96858.1"
                     /translation="MVVAKFCLVGFVFAAPEIRDDAVRLDFFFSPGCEECERVKREAL
                     PELADQLDGLYELASHDLTDAESVPLLVAYQQRCGNTDNGRVALVIDHSVFLSGADLI
                     VTGLLDRVGEALVRRQQPGWQMPQAPILDDERAAEIVKERAGALTFSVVALGGLLDGF
                     NPCAISTLIFFMSVLALARADRRTRLLVGASFITASFFVYMGLGMGFLFAFRQVPQFV
                     MVKRIIEVVLGLAMVPLAILSFRDAIRFRKSQRPDDVTLQIPKKIKGQIHAFMNSRLG
                     VGGPVLGGLVTGAGVTLLESVCTGQSYVPVLMYMLKRDCSDFTSWILLITYNLLFVLP
                     LTVVFICFHRGLQLTSLINWSKRNLVVVKILLGFFFAAMALLLLWGKT"
     gene            6479..6652
                     /locus_tag="PLZ74_00630"
     CDS             6479..6652
                     /locus_tag="PLZ74_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96859.1"
                     /translation="MANVHVLQIAIGIGIGIAIGFEIDCCAHAVARSDKCNDSGDSDS
                     DTDGDSDGAAMEP"
     gene            complement(6815..8953)
                     /locus_tag="PLZ74_00635"
     CDS             complement(6815..8953)
                     /locus_tag="PLZ74_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOR96860.1"
                     /translation="MERITRRKFLKTVGAAAPAAGWVTGGAASPDAGGTEAFREAVTR
                     YLETLARSDGGYGWEDQEDSFLTETYAAVGAYHALGMRPPRPAETAAYVRRAHPINGP
                     YAQTRRHWAEIKEFVFQQIQTLLWLGEPAGEGFSRQVAGWRKVSGYTTAYEKGGNPVF
                     RQEVQPLLCRVLLGLPCETLAEAFGGYLEARRRPDGTFNNTPAADGSGGHLVNTFFGI
                     RAQQALGIAPRPAALDWIRRCQRPDGGFSWCPEPEVGGLSDLSYTWAAIRTLELLGGK
                     PVRPEACRDWIRSLWNADGGFADRPGGVSSALATYQACDILARTGGGLRDTGPQDRPS
                     RRRSVIPADLHAFTIQFEAPGEGSVIEAVELAKALKIHLWGAKNSRPGWREAAQACAE
                     ERGVPVTFFASDEAYGTRVSLPGLGSYTHVNDPVAAPGRPVPAWPRRDGSWREFRDEC
                     VGPLHAAGGMMLWQICDNEEFARIVLDDSILRGGYGAISTFHFGCHNMIWALPFMMRY
                     RHEIPFVSLQDAHGESWWWSGMLTGFRTLFLAREPGWEGWQEALREKRVVAVRRDART
                     AGRIRMLGGAPEVREACMRRLDDWRWWDDSGTVLDRMPVSVVTVRPGDRFEFGRPDAG
                     VCVRIRVRREWVEGRKLADPSAITCVGATLDGVAIKPERVAKRDAQGLLEECCELIRL
                     PASSAKRILNMNFREQTADGVREFTRRLEV"
     gene            9188..10324
                     /locus_tag="PLZ74_00640"
     CDS             9188..10324
                     /locus_tag="PLZ74_00640"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOR96861.1"
                     /translation="MINLKQALLTLSGAMCVFALPVRAEKILVFFGTGGAHAQGIYRA
                     SFDTRTGRLEPAALAAKIGSPGFLALHPDGDKLYAAANGEGGQGVTGFRIGGDGVLNA
                     FTAAPTGDGQACHLAVHPSRRLLLTAQYGTGSVALFPLDTEGRLGAATTHKHPGPGTG
                     VVAQRQKTPHPHWCGFSPDGRFALVPDLGLDGIMVYQIDATAPNIARHGFIAAPRGAG
                     PRHMRFSTDGRRIHLLNELSLTVSTYAWDAETGSARLLTTVPTLSETEKAGEAHNSAA
                     EILTHPNGRFLYASNRGNDTVTVFRADPATTAPERIQVQPVRGAFPRNINLSPCGNWL
                     LAAGADSHTIAVHKVDPETGRLTYQTRSVISVPAPICILFARVP"
     gene            10646..11821
                     /locus_tag="PLZ74_00645"
     CDS             10646..11821
                     /locus_tag="PLZ74_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOR96862.1"
                     /translation="MCPTRNPSDPKAAIAFTERQQGIVAAGITVLCATLITGFAVLLF
                     WALFALLSAVSVVLTPLLVAMILALLFKPYYQWLHAHLRRSHLLALPAFFLSLLIPVG
                     GLFFFFGSLLVTQLMALYDYLPSLIEQIRDALTHGNPALEAFITKFGLETKIPLLSDP
                     ESFVAALFDRVSLDSMGGMALAYGMDAIKHLVSLIGWLVVPVYLIYFLTAKPFSGKNA
                     EDFLPFLKPATRRDVSYLIDEFFSIIVAFFRGQVTVAFIQGLLFGFGFWIVGLPYGLL
                     IGLTLGLFNLVPYLGNIIGLAVTLPLAFFGEGGSGLRLGLVLAVFCIVQILDGYFITP
                     RIQGKKTGLSDPAVIFSLLFWGVVFKGILGVLLAIPLSAFIVVLWRLLKTKYIRELI"
     gene            12202..13170
                     /locus_tag="PLZ74_00650"
     CDS             12202..13170
                     /locus_tag="PLZ74_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR96863.1"
                     /translation="MSLPLLEVENLAVVFRQEGVETVPVRSVSFTIRAGERVAIVGES
                     GCGKSLTAMSLTRLPPTDRARLSGRVRFDGTDTLALNHAGLARVRGRGIAYVFQDPSG
                     SLNPVMRVRDQIAECLPDLRRADRADAVVRLLTQTGLADPLRAARAYPCELSGGQQQR
                     VMLAMALAARPKLLVADEPTTALDVTTQRQVLDLIDTLADACGMAVLLITHNLGIVAG
                     RMSQVHVMYAGQIIESGPVTQVLTRPRHPYTRGLLAAVPLLDGPRDAPLQDIPGRVPS
                     PDVWPPGCAFAPRCPEASERCSAQSPPKTGDATHTCRCWLEEAPCS"
     gene            13161..13964
                     /locus_tag="PLZ74_00655"
     CDS             13161..13964
                     /locus_tag="PLZ74_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR96864.1"
                     /translation="MLLDVRDLHVLYRTPGRQPVAAVRGVTFSLDTGESLGLVGESGC
                     GKSSLARALIGIQPPEAGTIRFEGQDIARLTPPAFARYRRAVQMIFQDAAGSLNPRMT
                     VRQTLEEVLRVHAMRPAARIPDRIRELLDRVGLPETVLNAFPREISGGQCQRVSFARC
                     LALEPRLILADEPVSALDVSVQARILNRLRSLQKELNLAIILISHDLAVVRNICDRVV
                     IMRAGEFVECGTANEVLDTPQHPYTRELLAAVPDIARSLRSKVPPQPVT"
     gene            13986..14552
                     /locus_tag="PLZ74_00660"
     CDS             13986..14552
                     /locus_tag="PLZ74_00660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOR96865.1"
                     /translation="MPPPLILGSASPRRSKILQALGCVFTVSTVDTKELHDDFDPIRA
                     VTFNALEKHRACRLRHPDSALITADTLVWFEGRLIGKPADFDEAAELLRSFSGRTQIV
                     YTGVALSLPGQEPELRVEASSVRFRELTAAVIADYLHRTRPLDRAGAYDIDENGDLLI
                     AEVCGSYTNVMGLPEAPVRDWLRAHGLI"
     gene            complement(14545..14919)
                     /locus_tag="PLZ74_00665"
     CDS             complement(14545..14919)
                     /locus_tag="PLZ74_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013459605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOR96866.1"
                     /translation="MRKISTETAPAVIGPYAQAVEANGFLFCSGQLAVNPASGMIEMA
                     GAAEQTMQVLLNLRAVLEAAGSGLDKVVKTTVFLTDMGDFPAMNKVYAEMFGSHTPAR
                     STVQVTRLPKCAKVEIEAIAVI"
     gene            complement(14946..16877)
                     /locus_tag="PLZ74_00670"
     CDS             complement(14946..16877)
                     /locus_tag="PLZ74_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOR96867.1"
                     /translation="MTRRLTMWMVMAALTAGAAPVAVIHGYQSVSEGERRFAASLARH
                     VERWYREVGVEAVLTDDRDLSRSLASARVAVLVYLSQPNAMQLAALKAFAGRGGSMIV
                     CYSSSAELAALLGMETVGYQKGSTDGRWSMMRFSAQRPPGTPERILQTSRNLFRVQPV
                     AGRSQVLAWWHDRQGRQTGEAAWLMSPNGYWMTHVLLADGDADAKGRLLLALAAARDP
                     SLWRPAAARVLDRARGIGGAGGAEGLRMNAERLTDPARRTRALNAARGALRSEAETVR
                     ALAAGKGYEAWLAAQDLRARMYEAYGAAQPVRKGEFRGVWEHSGMGLYPGDWSRTCRL
                     LKDAGISDLFVNVAGAGFAHYASGVLPRSRVFDEQGDQLAACLAAAKPLGLRVHAWLL
                     CFSTEGATNDRIGIFAKRGWLLTHPDGTPRPWLDPAVPAVREYLLKAVREMATAYKTD
                     GVHLDFVRYPDYASSLGPTVRTRFEKATGRSAADWPEDVKSGALRASFIRWRAERVTD
                     LVQASRRTLRRDAPGKLLTAAVFGKYPSCLDAVGQDWESWINIGLVDYVMPMNYTEDL
                     SRFNEWLSQQTRLRKQTMRVLPGIGVTAAESRLDAAQVIDQIRAVRRAGCPGYLFFDL
                     DTTLHQEILPVLRLGVTAP"
     gene            complement(16910..18217)
                     /gene="hisD"
                     /locus_tag="PLZ74_00675"
     CDS             complement(16910..18217)
                     /gene="hisD"
                     /locus_tag="PLZ74_00675"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOR96868.1"
                     /translation="MRATIAQWSLTRPTPKVERFLARPAFSPEAETAAAAVLAEVRAH
                     GNRAVLAAARRFDGAALRVSEMRVTDAELKAATREVPARVRRAVNDAHTRVRRYAEAS
                     LREAWHMPLPRGGFTGEFFSPMGRVGVYVPGGTAPLASTAVMTVTLARTAGVREIVAC
                     TPCGRDKRVNPVLLHALRVAGATEVYRVGGIQSIGLMAFGTATVRPVQKIVGPGNAYV
                     TAAKRQVYGIVALDQVAGPSEIAILADDSAAPEWVAADLLSQAEHGSGHEKALLVTDS
                     LVLARAVRDALTAQTRLLSRAACIERVLAVGGILAVVVPDLDQGMELVNRFAPEHFEI
                     MTRDARLRLNQVRAAGAVFVGPWTPEAAGDFVAGPSHVLPTGGAARMFNGLTADDFRR
                     RHSFVEFTREDLIETRRTIETFAEVEGLDAHGRSARIRFEERC"
     assembly_gap    18353..18452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18453..18997)
                     /locus_tag="PLZ74_00680"
     CDS             complement(<18453..18997)
                     /locus_tag="PLZ74_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96869.1"
                     /translation="MRNGLMQMAMVLMAVGLARAEMVSLVQITDMRGQTGFEILNREE
                     YTALQKEIREEAAAYTVAAAESKKQWDANKENKLPFPGNKIKPRSAKKMGADFPNREK
                     AETRKARLEERINEKQLEEMDKESRRKKMMKDEDLAKEASREKAFEDAFAMISKRMAE
                     KLGRPVPTFGVTFVEPAKDEPA"
     gene            complement(19045..21096)
                     /locus_tag="PLZ74_00685"
     CDS             complement(19045..21096)
                     /locus_tag="PLZ74_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR96870.1"
                     /translation="MSLGFIKKIADKLLGQKQQSQKKPAAKTGRGAKNPKREPSRNGR
                     RSRPQGGRAKPQASKEGSAGRSSRGASGREGARKADAGRGKPRRESSGRGSETRPRRE
                     SGPPAVAAAARSVARPPSKEPRRGRGRGGGERSDPNTRRPGGAVGDSERAARIAEHAS
                     WSPESFSVEPAEGKKRFHDFDLPGEILHAVADLGFQYCTPIQALSLEHALAGKNVAGK
                     AQTGTGKTAAFLVAILTRYLRSPESRQQKGGAPRALVIAPTRELVIQICKDAEALGKY
                     CGLRSLAVYGGMDYDRQKREVSDAPVDLLVATPGRLLDFVRSRVIDLSKVDTLVIDEA
                     DRMLDMGFIPDVRSIIARLPPKNRRSTMLYSATLTEEVMRLALQWMEAPVKVEVESEQ
                     VTTAAVKQVVYVVTVNEKFTVLYNHIQQYPDSRMLIFCNRRNTTEDVADSLTRRGIRC
                     EMLSGDVNQNRRLRVLEDFREGRVRIVVATDVAGRGLHVDDIGFVVNFDFPYEPEDYV
                     HRIGRTGRAGASGVAISFADEDESFIIPEIEKYIGEELKCTVLAPDDPLLAKLPPKPA
                     GRSRRASLSESANSRRPAGAQRERAGEGGTAVRGDARPRRSEPSPAAVPANAAQPKEP
                     AAAKPEPKPEPRKPEKPRPAPARRAETPARSAVTAAPRLAVAQRRPRFSEEWVPGQQS
                     K"
     gene            complement(21180..21572)
                     /gene="rpsI"
                     /locus_tag="PLZ74_00690"
     CDS             complement(21180..21572)
                     /gene="rpsI"
                     /locus_tag="PLZ74_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOR96871.1"
                     /translation="MAKEIAAGTGRRKTAIARVRLVPGDGKWFVNDVAIDQYIPSEAL
                     RQYLSQPLELTGMQGKYDVFVSAKGGGISGQSGAIRHGLSRALVASDATLRDVLKKAG
                     CLTRDARMKERKKPGQPGARKRFQFSKR"
     gene            complement(21635..22066)
                     /gene="rplM"
                     /locus_tag="PLZ74_00695"
     CDS             complement(21635..22066)
                     /gene="rplM"
                     /locus_tag="PLZ74_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOR96872.1"
                     /translation="MTKTTKPKDPGPNRSWHLVDATGKPLGRLAVNIANTLRGKNRPT
                     FDPSVDTGDFVIVVNAEKVLLTGRKEEQKEYQRYSGWRGGLKRFTAKTMLEKHPDRVI
                     KEAVWGMLPKNTLSRKMMTRVKVYRGAEHPHEAQMPVAATF"
     gene            complement(22153..22698)
                     /locus_tag="PLZ74_00700"
     CDS             complement(22153..22698)
                     /locus_tag="PLZ74_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96873.1"
                     /translation="MSLSNWCYLLGLFCLSLGVTIVLCPGPAARVFRAFPRNRAAGIV
                     LSTLAWIWAGAALWTMGLDLLMPFKRYIPFAVLVCIPLTWFWLDNLLPCRALGGLLTL
                     FPYDLLHTARTHPSPWRLVVVAVAYLCIIKGMVLLLYPWKLRQAIDWLTGRPGLFRAA
                     GFLQGLMGALLAALGASVLRV"
     gene            complement(22767..23528)
                     /locus_tag="PLZ74_00705"
     CDS             complement(22767..23528)
                     /locus_tag="PLZ74_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOR96874.1"
                     /translation="MRLLLHNIQYGTGRLRRFAWVETLLRTTAHFPNITRFVRHMNPD
                     IVGLVEVDAGSYRARHRNQAEELAADLSLYHCCRVKYPDEGMGRRLPVFNKQANAVLV
                     REPILRTRFHDFETGFKSLAIEVELENVTFFLVHLALGIRARQRQLSDLYDLITGCDK
                     PRLVAGDFNTLSGVWEMRMFLRATGLRSANTRHAPTFPSWAPCRELDFICYDPSIITP
                     VRFKIPRVRLSDHLPLMFDFEIGGGRIRAPGDGGH"
     gene            complement(23536..25335)
                     /locus_tag="PLZ74_00710"
     CDS             complement(23536..25335)
                     /locus_tag="PLZ74_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOR96875.1"
                     /translation="MKKISLALFPVLALALFAAAWLWLFCRIPVPAGHMAIITRKTGH
                     PLPPGQILAGPGEKGVQRDPLPEGRHFRDPVTHSWRIVPLVSIPVGKVGVVTAKVGRE
                     LPPGEILAADKEGKGVWKDVLGPGTYRLNPEGYEITQLDAVSIPIGYVGIVTSQSGEP
                     VKTGEFAGPGQQGVMKDVLQPGLYYVNPRAYQVDVLEIGMNQVSINGRSGSVVLTKSQ
                     ISNASSALDELHATTIQKQQQKRADYIGRNSNLLAQNEVAAALGKSREILGKALSRKV
                     SAASPRESKAYAMEAPGGRRRAPETEDLLADTDGPAAMRQAVVPESVAFGINRFVEFP
                     SRDGFQILLDMTVEFELLPEDISRIYMLYGDLPAVVEKIILPQVLSISRIRGSSYKAR
                     DFIDGEGRQLFQKELTAELTRVMGEKHIVIHNAIIRHVEVPGEILTPIQDSSLAKEQD
                     LTNKARQDTARKQAELNTETAMIEQFKKQVEQETEKLVATTVARTRSEVANIQAGTAL
                     AVAATNLLKAAVQAQITRVQGEARVRADYLTANEKALGEQLRAGVFKDPATLADLTFV
                     DKLNPDAGIRILHAGEGTLWTDLKSLAPSLPVK"
     gene            complement(25332..26933)
                     /locus_tag="PLZ74_00715"
     CDS             complement(25332..26933)
                     /locus_tag="PLZ74_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOR96876.1"
                     /translation="MQEDAEIMPPPLENRRSAPSRRTPSRRAALSSLSLAPLLLMLAL
                     ILGPIWFWFWWRIEPGNGKMAVLFRKTGKNLPVDRIIADEPGYKGIQLEVLPEGRFFR
                     NPYTWGWQIVRVTDIPAGKFGVLVRKYGKDLPGGEILAPDEGYKGIVRDVLGTGKHRI
                     NPYAYEVRLFDDIKIAPGHVGVVTALCGRDILSGIDGSDVVREKGFLAAPGCKGVQSE
                     VLKEGTHRLNPFLYAVSIVNIQSQRFEFSGEEAIMFLTLDGFPVTVEGTLEFNLNIDK
                     VALLTHEVGDMDDIRQKLILPSARGFFRIEGSKKSATEFIVGESRQAFQDSLEKYLRG
                     ICANWGVSLNSVLIRDIIAPQQVAGIIRDRELANQESLKFEQQIVQARSQADLEREKA
                     LAIQNSAKVTAETERIRQTIAAEQVRIEQTLAARTELSVAEIQLEVARADAEALLQLA
                     EADRRVIEAQNRANADVLKQQVAVYRDEADYVRARLYEKTAPKIQGVLTSDDGQGLFG
                     LPVGAKGGPAAPAAGTVKPAKGDTP"
     gene            27343..28848
                     /locus_tag="PLZ74_00720"
     CDS             27343..28848
                     /locus_tag="PLZ74_00720"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOR96877.1"
                     /translation="MTTSSAITPHAVAAHLGKPPAQFTKADIIRFIQDRGIQMVNFMY
                     PGGDGRLKTLNFVINDLAYLDAILTCGERVDGSSLFSFIEASGSDLYVVPRFRTAFLD
                     PFTDIPTLTLLCSFFNKEGRPLENSPDYTLHKACKAFTDITGLEFQAMGELEYYVVAD
                     DVALFHAVDDQRGYHESGPYAKFNAFRSHCMLTIAQCGGLVKYGHSEVGNFTQNGKIY
                     EQNEIEFLPTCAEDAADQLVVAKWIIRNLSRQRGLNVTFAPKITVGKAGSGLHIHMRI
                     MKDGRNRMLADGALSDTARKVIAGLMTLAPSITAFGNKNPTSYFRLVPHQEAPTSVCW
                     GDRNRSVLVRVPLGWTAENDMSAVANPLETASVRDTSDKQTVEMRSPDGSADVYQLLA
                     SLAVAARHGFEMPDALDVAAKTYVDVNIHKTGNESIAASLSQLPGCCVDSAASLERQR
                     AVFEAHDVFSPGMIDGVVAALRAYDDKNLRAELMKSPERLAELVERHFNCG"
     gene            29062..29706
                     /locus_tag="PLZ74_00725"
     CDS             29062..29706
                     /locus_tag="PLZ74_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96878.1"
                     /translation="MNEENEKPVKTREELAAIAYLDKLRRGVGETMPTELPLACVVKV
                     VAYKEIPKANEKYALVTVRGPIGITWKMCVNRHYLKKGMNALFVSKGAAMPEEERFRN
                     PEAAKVKLRVFKFGPGIKERRLLPIVSRNIYLFNCGLLYPLDDFPELQGVRAGMVCAV
                     KLRIDRLKELHERVAAPKPKQKKKPAPAPKAESDGDDSVLAKVRRYYIRVWRAR"
     gene            complement(29725..29958)
                     /locus_tag="PLZ74_00730"
     CDS             complement(29725..29958)
                     /locus_tag="PLZ74_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96879.1"
                     /translation="MSDRGDGRQARRTDRDIRDGTYAPDAASLATACHVKVWRAYLLK
                     LEVRRDVPDRRKRAWCLTLRGTVLPPHKLRGFA"
     gene            complement(29951..32452)
                     /locus_tag="PLZ74_00735"
     CDS             complement(29951..32452)
                     /locus_tag="PLZ74_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96880.1"
                     /translation="MCWKRWCSTAVVWAVAGAAGAIFAGVRAPSGGPMVTGQYAFVSG
                     IYPHLAMYNGGGECGIGAVVPWAGRLWTLTYSPHSPTGSDDKLYEITPDLVSVVRSES
                     IGGTPANRMIHPESNQLFIGPYAIGADRTVRTIPYSVMYGRPTGNARHLTDPEGKIYY
                     ASMEEGFYEVDVRTLAVTELWRDEQLAKAKRTPGDNPRFAKLPGYHGKGLYSGQGLLI
                     YANNGDQARQAFVDPSAPSGCLAVWDGKAAAWTVVRRNQFTDVTGPGGILGNPNPETD
                     PVWAIGWDHRSLLLTVLDCGAWHTYRLPKGSHSYDGGHGWNTEWPRIREIGGKSFLMT
                     MHGTFWRFPKTFSAHDSAGIAPLSNYLKVVADFCRWGDWLVLACDDSAKSEFLNKRRA
                     KGTIRGPGQSHSNLWFVNPERLDAFGPAIGRGAVWLGESVSAGVPSDPYLFSGYAVRS
                     LGLAHGSAEPVRFTLETDRAGKGVWKAVRTVEAAPGRLTALTFAERERGAWVRLVPQR
                     DAADVYACFQYANPDRRSDRPDARCAGLASLRDPRAYGGIVYARGGDKRVLGLAAVTA
                     GPDGVRETGYYELDADLNLTRKEDAAAWKVARTDYPMSGTGLVVEAASALITDDAGRR
                     WRFPKADPAYAAPGLFGATRLCREVCTERDLLNVCGIFYELPAENAGGFSKVRAVATH
                     CGQVHDYCSYRGLLVMTGIRENAATSRHLIRSGDGRAAVWVGSVDDLWAFGKARGEGG
                     PWKDSVTAAGVPSDPYLMTGFDRKRLALSHAAAGIVTVTLELDLTGTGVWRVYDTYRV
                     APGQPTRVVLDDARAYWLRVTADRACTATAWLTYE"
     gene            complement(32547..>33010)
                     /locus_tag="PLZ74_00740"
     CDS             complement(32547..>33010)
                     /locus_tag="PLZ74_00740"
                     /inference="COORDINATES: protein motif:HMM:NF028447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HOR96881.1"
                     /translation="TKEIQIPEGLWHDWKTGELVTGPCKRAVKVTPEWGGSLFVRAGA
                     VIPMWEKKAHVDKGWDETVELHVWPGADGKGDLYEDDGVSLEYRSGKGALTTCAFKDG
                     ALTVGPRTGTYSGMPATRKMKAVFHKANSLETVTVELGDVGTAGTGPVRCP"
BASE COUNT         6027 a        11279 c         9665 g         5939 t
ORIGIN      
        1 tcatttgttt ttgcctttcg ttagcacgtt cacatgtgac cgctcgaatc tgcggcgggc
       61 gatacggcga gacgcccgaa gaacggcaga cgcgtgagcg gcgtggcctg attcccgtcc
      121 gtttccatgt ttaatctatt atgcggtcgg gggacgaaag cagtcaaccc cgaacccccc
      181 cggaacgtgt tgaacggtga gggcggcacc cccttccatg cgggacaaaa ggtgtggcgc
      241 acggaatccg acctgcggcc ggaaccgcgg atacgatgct tgcacccgtt ccggccatcc
      301 gttattctct tatcgaatcc gacacgtttc agaaaggaca cgactcatga cacgcaccct
      361 ccgtttgatc acgatctgtt cgctactcgc cgcatgcgcg ggcctgctgt cggccgcacc
      421 cgaggccgaa gcctaccgca tccgccgcgg catcccgaac agccggtact ttctccagaa
      481 cgacaccgtg ggcaaccagt atctcttttt catcggccat tccgtgcttg cgggaaccgg
      541 actgaaaaac tcggggctgc gttattccgc gtgtatgacc aaagccctca aacggcactt
      601 cccgaacgcc tcgccccgcg agacgcgcca catgtacccc ggcggcagct ggtttgccca
      661 gtaccgttgt agccgcggcc agccggtttt cggtgaggtg atttgcagtg gccacctcgc
      721 gattctggat ttcgcggctg aggatcgaag cgctgatctc gaacaggtca aaacctccct
      781 tgagggcctg atccgtcaga tcacgctcta ccgcgccacg cacagcacga tcctcgtata
      841 cacgctaacc cccgagatgc tggccgacta ccgtgcgggc cgcacgcccg cgtacatcga
      901 ggtctgcgag cggatcgcgg accactacgg cattccgagc ctgaacctgg cccaggtcgc
      961 ggcccgcgcg atcctttccg gcacgatctc tgccgaggct ttctccgctg acggcatcca
     1021 tcccacggac gcgggtgcgc gtctgtgggc cgaggcgatc aatgcctttg tcgacgcgct
     1081 catggccgac ggtccggttc ccgaaaaacc gcagagggtg accctccccg cgcccctgtt
     1141 ccccggaacg gatgaccgtg gacaaatcat cgcgtatgaa cacccggagg tacgcaccac
     1201 cggttcttgg caaccgggcc gggagagtcc gatcaagccc ttccgccatg tgctggtttc
     1261 ccgcgagccc ggcacccggc tgacgctccg gttccagggt tcggaaatcg gcctcatcga
     1321 tgtcgccgct ccggacggcg cggatctcga atacgcggtg gacggcggcg cgttccgcac
     1381 cctcgcggca ccgaagaaca cagacaagcc cgtcttgcgc gcgctcccgc tcgcacgcgg
     1441 cttgacgcgg gatgccgaac atgaggtcgt catccgcacc gcttcggacg gcacggcgcg
     1501 catcggcgga ctgctggtca acggcagcgt gaagaacccc cacgccggaa tgagcacgct
     1561 cgaacggatc gacgcgatct acgccgcgat ggacccgatc aactacacgc cgcctgcaaa
     1621 ccgctttgcc cacatcccga aaaccatggc caaactgcgc gggggcggcg aattgcgtat
     1681 ggttctgctc ggtgacagca ttatgggcaa cacctcgggc tcctcgttcg aactgctgct
     1741 gatgcgcaac gcgcccgcct gcaaaatcat caaaatcgcc tccctgcgca gttccaccgg
     1801 gtgcacctac tatcagcacg agaaccgcgt acagcagtat gtgctgcgcc acaaccccga
     1861 tctgttgatg atcggcggta tctccaacgg cggcgatgcc gaggccgtgc gttccgtgat
     1921 ccgtcaggtc cgggcctcga agccggatac ggaggtgctg ctgctcacgc ccgttttcgg
     1981 cgccatgcgt gatgagcaca tccggacctt cacccgggag atcgacacca cgaccccgaa
     2041 cttccggcac cgtctcagca aggtggcgga agaagagcgc tgcgccttct tcgatatgac
     2101 cggcccctgg tgggaataca tccaaaagag cggcaagacc tacgggtggt tcatgggcga
     2161 cgccgtgcat gccaatgcgc gcggttgcca gatcatcggg cgtctgctgg agcgctggtt
     2221 caaggagtga cgcggttgga attgccggaa gggcgcgcgc ccgcccggta tgcggcttcg
     2281 cgtgccagac aggcctccac agctgcgcgg acctcctcca gcgtgtggag cgtgtgcaga
     2341 cggccgcgca gatacgagga gcctttgagc ccgtgcaggt agcggaaaag atgcccccgg
     2401 aaagcggcca cccctgcagc gtccagatcc ctaacggaac cggcgcggcc ctcgcgttcg
     2461 caacagcggc gcgccaattc gcattcggct tcgaggtgcg cctccaaaac agaccggatc
     2521 gtctcaagcg cgcgccgtgg acgaccccgc gccgtttcga cgggtgacgg ggtcctgccc
     2581 gagacgaggg cgtcgcgaat atcgctaaaa agccaggggt taccgatggc ggcgcgcgca
     2641 accatcaccg catccacacc ggtctcctgc agcatgcgtt gggccgtata cggcgaaccc
     2701 accccgccgt tgccgatgac cggtatccgg gcgcggcgtt tgacctcagc aagcagatcg
     2761 agccgcgcct ccccgccgtg tccttgcgag gtgaaccgcg cgtgcagcgc aaaggcgtcg
     2821 gcctccgcct cctctgccgc ggccaacagc tcgaaaaccg tcacccggcc cgggtcgggc
     2881 ccgaggcgtg ttttcacggt cagaggcaac gtgaccgcgc ggcgcatggc ctttaggatc
     2941 gcgtgtacgc gttgcggatc cgccgccaga gtcgcgcccg cgccggtcgc ggcgatcttc
     3001 cgcaccgggc agcccgcgtt gagatcaatc gcggcaaagc gtcccgtggc ttccacgcgc
     3061 acggcggcct cggccagaac ctcgggctcc gaaccgtaga gatgggccac aaccggccct
     3121 tcgtcggcaa acgtctccag cagatgccat gttttgtccg aggagtgcaa cagacccgcc
     3181 gcgctggtca tctccgtata ggtcagttcc gcaccgtgcg cacggcacag cgcgcgcatc
     3241 ggtgctccgg tgaatccggc catcggcgcc agaatcagcg gcggtaccga acgatcggag
     3301 agggagaata tgggtagaga aggcataacg tccgcgttga gatgtgtaag gtatccccat
     3361 cccgcagggt tttcaaccca acaaacgtct tttgtccgtt tccgatgatg atgcgcaaaa
     3421 agaaccgctt gctggatttg accccgaagg ccccgcccgc gtatacttga agccgtcatg
     3481 cgtgaagttc tcgatgtctt gctcctctcc gtgctgcagg gaattgccga attcctgccg
     3541 atcagcagtt ccggccatct ggtgctcgcc caaagcctgc tgggcgtcaa caccccaggc
     3601 atgcggttgg aggtggtgct gcatctcggc acactggtct ccatcctcgt gtattaccgg
     3661 aaccgcctca cgcagttggt tctcgggacc gtgcggggcg accgcgaaag ctggcgcaca
     3721 gcgggacaga tcgcgctttc cgccatgcct gcggttttct tttatctgct ctgccacgac
     3781 aagatcaacg cctttttcga gtccccccgg gccgtgggcg ggtttctgat cttcaccggc
     3841 gtggttttgt acgcgctgcg ctggatggcg tgcggccaag gcagactcac cgccgcgcgc
     3901 gcgctgctgg tggggctggc ccaggcgctg gcggtcctgc cgggcgtcag ccgttccggc
     3961 atgacgatcg cggccggacg catggcaggc atcgcgccgg accgggccgc ggagttctcg
     4021 ttcctgatgt gcataccgct cttaatcggc gcggccctga tggatggact gggcatcacg
     4081 ccggcggcac cgaaagccga tgcgctgccc gtatggctgc tgttggcggg tgccgcagtt
     4141 tcggcaggcg tggggtatct cgccctctct ctgctcgtgc gtgttttgcg tgcgggccgc
     4201 ttctggctgt tcggcctcta ctgcattgcc gcaggtttcg tgacaatcct gctgacctga
     4261 gtgggggacg cgtcactgcg caccgcgcgt ccagcgcgca aaggccccgg acggcgtgga
     4321 cggcacggtg gccgcgccag tgagcagcat ctcgcctgaa acgattttgc cgtccaaatc
     4381 ccccaccgcc tgcccgagga cgttctccag cacgagcggc gaatacccgg gagtgtgcgc
     4441 gctcagcagc acgaagagcg ggtcgggggc gagcaggcta cggcaaaggc ggagtgtctc
     4501 gggcaagtcg cgttcgatct tgtaggtttc gttgccctgt ccacggccga aggtgggcgg
     4561 atcaaggatc accgcatcgt aacgccgttc acggcgtacc tcccggcgca ggaaggtgtg
     4621 cgcgtcatcc gtgatccagc ggatcggatg cgcttcgagc cggttgagcc gcgcgttgtc
     4681 ccgcgcccac gtcaccatgc ccttcgaggc atcgagatga cagacctccg caccgcccaa
     4741 ggcggctgcc agggtcgagc ccccggagta ggcgaagaga ttcagaatcc gcaccggccc
     4801 cggacgcgcg gcgcatgcgt cccgcacggt gttgcggagc cacagccact gcgcacgctg
     4861 ttcgggaaac acgccgaggt ggccgaaatc cgtgcccgag agccggaaac ggatccccgc
     4921 gatctccacc tcccatgcgg cgggcaggcc actgcggttg tgccagcggt tcccatcctc
     4981 acggtcaaac gcggcgtgcg ccgccgcggt ccactccgcc tcgctcaggc ggggcttcca
     5041 aaccgcctgg gagcagggcc gggcgagcgt gtacgcgccg aaacgttcga gtttccggcc
     5101 atccccgctg tccagcagtt catattcatc ggccagaccc ggactcatct tccctctccg
     5161 caactccttg cgccggtcac gttttccccc agagcagcaa caacgccatc gcggcgaaaa
     5221 agaagccgag caggattttg acgaccacga gattccgctt gctccagttg atcagcgagg
     5281 tcagttgcaa cccccggtgg aaacagataa acaccacggt tagcggcagg acaaagagca
     5341 ggttgtaggt gatcagcagg atccaggaag tgaaatcgga acagtcgcgc ttgagcatat
     5401 acatcaggac cggcacgtaa ctctgccccg tgcagacact ttccagcaat gtgacccccg
     5461 cgcctgtgac cagtccgccc agcaccggcc cgcccacacc gagccgcgag ttcatgaacg
     5521 cgtggatctg gcccttgatc ttcttcggga tctggagcgt cacgtcatcc ggccgctgcg
     5581 atttgcggaa acggatggcg tcgcgaaagg agagaatcgc caacggcacc atggcaagcc
     5641 cgagcaccac ctcgataatc cgcttgacca tgacgaactg cggcacttga cggaacgcga
     5701 agagaaaacc cataccgagt cccatgtata cgaaaaagga ggcggtgatg aacgaagcgc
     5761 caaccagcag gcgggtgcgt cggtcggcac gggcgagggc gagcacactc atgaaaaaaa
     5821 tcagggtcga aatcgcgcag ggattgaacc cgtccagcaa accgccgagc gccaccaccg
     5881 agaaggtcag cgcccccgcc cgctccttca cgatctccgc cgcgcgctcg tcatccaaaa
     5941 tcggtgcctg cggcatctgc caacccggtt gctgccgccg caccagtgcc tcgccgactc
     6001 gatccagcag acccgtgaca atcaaatccg cgcccgagag gaacacgctg tgatcgatca
     6061 ccagcgccac gcggccgttg tcggtgttgc cgcaccgctg ctgataggcc acgagcaacg
     6121 ggacgctctc ggcatccgtg agatcatgtg aggccaactc gtagagcccg tcaagctgat
     6181 ccgccaattc aggcaacgcc tcccgtttca cccgctcgca ctcttcgcat cccggggaaa
     6241 agaagaaatc caaacgcacc gcatcgtccc ggatctcggg cgccgcaaag acgaacccga
     6301 ccagacaaaa cttcgcgacg accataagcc gcaaaaaccg aattactctg aaaaggtctt
     6361 tctgcatagg aataattata aaataccgca tatatggcaa agaccacaac ctaaaagtcc
     6421 gaacgtcacg caatcggttc gtgacagggg ggagagtcat cgtgtacggg tacggggtat
     6481 ggccaacgtt cacgttctcc aaatcgctat cggtatcggt atcggtatcg ctatcgggtt
     6541 cgaaatcgac tgttgcgccc acgcagtggc ccgcagcgac aagtgtaatg atagcggcga
     6601 ttccgattcc gataccgatg gcgatagcga tggcgccgca atggaaccct gaaacgcatg
     6661 accttcgcac acccctgtca cgaaccgatt acgaacgtca cagatcgaca tttcgtgctc
     6721 cagtcactct caggcgtccg agacgtccgc ccgtactctg ttcggctcat gtccctgcca
     6781 gtacggcggg acgtcccccc cttccctctt cgcctcacac ctccaagcgc cgcgtgaact
     6841 cccgcactcc gtcggcggtc tgttcacgga agttcatatt caggatgcgt ttggccgacg
     6901 aggcaggcaa gcggatcagt tcgcaacact cttccagcaa accttgggcg tcgcgcttcg
     6961 ccacgcgctc gggtttgatt gctacgccgt ccagcgtcgc tccgacacac gtgatcgccg
     7021 acggatccgc caacttccgc ccctccaccc attcgcgccg cacccgaatc cgcacgcaca
     7081 cgcccgcgtc cggacgtccg aactcgaacc ggtccccggg ccgcaccgtc accaccgaca
     7141 cgggcatccg gtccagcacg gtgccggagt catcccacca gcgccagtca tccagacgcc
     7201 tcatgcaggc ttcgcgcacc tcgggcgcgc cgcccagcat gcgtatccgc ccggcggtcc
     7261 gcgcatcacg cctcaccgcc accacccgtt tctcccgcaa ggcctcctgc cacccttccc
     7321 accccggctc gcgcgcgaga aacagggttc ggaacccggt gagcatcccg ctccaccacc
     7381 acgactcccc atgcgcgtcc tgcagggaca caaaggggat ttcgtgccgg tagcgcatca
     7441 taaagggcaa tgcccaaatc atgttgtggc aaccgaaatg aaaggtgctg atggcgccgt
     7501 aaccgccacg caggatcgaa tcgtccagca cgattcgcgc gaactcctcg ttgtcacaaa
     7561 tttgccagag catcatgcct cccgccgcgt ggagcggtcc gacgcactcg tcgcgaaact
     7621 cccgccagct cccatcgcgc cgcggccacg ccggcaccgg acgccccggt gccgccacag
     7681 ggtcattgac atgggtatat gaacccaacc ccggcaggct cacccgcgtt ccgtaggcct
     7741 cgtccgaggc aaagaacgtc accggcacac cccgttcctc cgcgcaggct tgtgcggcct
     7801 ccctccagcc ggggcgactg tttttcgcac cccacaagtg gatcttgagg gccttggcca
     7861 gttcaacggc ctcaatgacc gaaccttcgc ccggcgcttc aaactggatc gtgaacgcat
     7921 gcagatccgc agggatcacg gaccgtctgc gtgaggggcg gtcttgcggt ccggtatccc
     7981 gcaacccgcc tcccgtacgc gcaaggatat cgcaggcctg ataggtcgcc agtgctgacg
     8041 agaccccgcc cggccggtcg gcgaacccgc cgtccgcgtt ccagagggac cggatccagt
     8101 cgcggcaagc ctccggccgt accggtttcc cgcccagcag ttccagcgtc cggatcgcgg
     8161 cccacgtgta ggagaggtca ctgagcccgc cgacctccgg ttcgggacac caggagaatc
     8221 cgccgtccgg ccgttgacaa cgccggatcc aatcgagcgc ggcgggtcgg ggggcgatcc
     8281 ccagagcctg ctgcgcgcgg ataccgaaaa acgtgttcac cagatgcccg ccgctgccgt
     8341 ccgccgcagg cgtgttgttg aaggtgccgt ccggtctgcg ccgcgcctcc agatatccgc
     8401 cgaacgcctc ggccagcgtc tcgcacggca accccagcag tacgcggcac aagaggggct
     8461 gtacctcttg acggaagacc ggatttccgc ccttctcata cgccgtcgtg taccccgaga
     8521 ccttccgcca ccccgcaacc tgtcgggaaa agccctcccc tgccggttcg cccagccaaa
     8581 gcagggtctg aatctgttga aagacgaact cttttatctc ggcccagtgc cgccgcgtct
     8641 gcgcgtacgg tccgttgatc ggatgcgccc gcctgacata cgctgcggtt tcagcaggac
     8701 ggggcggccg catccccaaa gcatgatacg ccccgaccgc cgcatacgtt tccgtcagaa
     8761 aggaatcctc ctgatcctcc catccgtaac cgccgtcgga gcgtgccaaa gtctccagat
     8821 agcgcgtgac cgcctcgcgg aacgcctccg tccccccggc gtccggcgaa gccgcgcctc
     8881 ccgtaaccca gcctgcggcc ggagcggccg cgccgacggt cttcaaaaat tttctgcggg
     8941 tgatccgttc catcgttctc ccttattgca gccaaatcaa cataccgtgt aatcataatt
     9001 ttagacgcga tccccgcttt gtcaacgatg acaccgtccc gagaaaaacc ggaggatacc
     9061 gatcactccg atccgacaaa tccacaacgg gcgagaacac ccgcgttccc gacaccgcag
     9121 atcgctggcc gcagggaaaa gcgtccggtc cttgtccggc acggggaagc gtgttacagt
     9181 aatccgcatg atcaacctta aacaagctct tctcacgctg tccggcgcaa tgtgcgtttt
     9241 cgccctcccg gtgcgggcgg aaaaaatcct cgtgttcttc ggaaccggcg gtgcccacgc
     9301 ccaaggcatc taccgcgcgt catttgacac ccggaccggc cgactcgagc cagcggcgct
     9361 ggcggccaaa atcggctctc ccggtttttt ggccttgcat ccggacgggg acaagcttta
     9421 cgctgcggcg aacggcgagg gtggtcaagg cgtgaccggt ttccgcatcg gcggcgacgg
     9481 tgtgctgaac gccttcacgg ccgcgcctac aggcgacggc caggcgtgtc atctggccgt
     9541 acacccctcc cggcgcttgt tgttgaccgc gcagtacggc accggctcgg tcgcgctctt
     9601 tccgctggat acggaagggc ggctgggcgc ggccacaacg cacaaacatc cggggccggg
     9661 aacaggtgtg gtcgcgcaac gtcagaagac accacatccg cactggtgcg gcttctcgcc
     9721 cgacggccgc ttcgcgctgg tgccggacct cgggctggac ggcatcatgg tttaccagat
     9781 cgacgcgact gcgccgaata tcgcacgcca cggcttcatc gccgcgccgc gcggcgccgg
     9841 accgcggcac atgcgtttct ccacagacgg ccgccggatc catctgctca acgagctttc
     9901 gctgacggtc tcgacctacg cgtgggacgc ggaaaccgga agcgcgcgcc tgctgaccac
     9961 cgtcccgacc ctgagcgaaa cagagaaggc cggcgaggcg cacaacagtg ccgccgaaat
    10021 cctgacgcac ccgaacggcc gtttcctcta cgcctccaac cgcggaaacg acacggtgac
    10081 ggtgttccgc gccgatccgg ctacaaccgc gccggagagg atccaggttc agccggtgcg
    10141 cggcgcgttc ccgcgcaaca tcaatctctc gccatgcggc aactggctac tggcagcggg
    10201 cgcggactcg cacaccatcg cggtgcacaa agttgatcct gaaacgggac gactgaccta
    10261 ccagacgcga agcgtgattt cggtgcccgc accgatctgc atcctcttcg cgcgtgtgcc
    10321 gtaggcgcgg agcgggaccg ttgaaaccgt gccggacttc ggggtaggga cgcctccccg
    10381 aggcgtccga ggacgcggac aggcaaacgt ctgccagcct ggacgggcag acgctcgtcc
    10441 cttcgtgatt tcatgaagtt tcttgtgttt catggttacg gatatttaca aggctttggt
    10501 tgcgattgct ctttttaggc ttataagatc acggctttcg cttgcaaacc gctgatgtcc
    10561 cacctacact ggcaactgtt gaaagcggag cgcccgacgc gggccgccgc agtttcagac
    10621 tgaacgtttc aggagtgcct gctttatgtg tccaacccgc aacccttctg acccgaaagc
    10681 ggcgatcgcc ttcacggaac gccaacaggg gatcgtggcc gccggcatca cggtcctttg
    10741 cgccacgctg atcaccgggt tcgcggtcct gctgttctgg gcgttgttcg cgttgctctc
    10801 ggccgtctcg gtagtgctca ccccgctgct cgtggcgatg atcctcgcgc tgcttttcaa
    10861 accctactat cagtggctcc acgcgcattt gcggcgctcg cacctgctgg ccctgcccgc
    10921 tttcttcctc tccctcctga ttcccgtggg cggactcttc ttttttttcg gctcgctgct
    10981 ggtcacccaa ctgatggccc tgtacgatta cctgccgtcg ctgattgagc agatccgtga
    11041 cgccctgacg cacggcaacc ccgcgctgga agccttcatc accaagttcg gcctggaaac
    11101 gaagatcccg ctgttgagcg accccgaatc gtttgtcgcc gccctgttcg accgggtctc
    11161 gctggacagc atgggcggca tggcgctcgc ctacggcatg gatgcgatca agcatctcgt
    11221 ctcgctcatc ggttggctgg tcgttccggt ctatctgatc tacttcctga ccgccaaacc
    11281 cttttccggc aagaatgccg aggacttcct gccgtttctg aagcccgcga cacgccgaga
    11341 cgtctcttac ctcatcgacg agttcttctc catcatcgtg gccttcttcc gcggtcaggt
    11401 aaccgtcgcc ttcatccaag ggcttctctt cggtttcggg ttctggatcg tgggcctccc
    11461 ctacggtctg ctgatcggtc tcacgctggg gcttttcaac ctcgtcccct atcttggcaa
    11521 catcatcggt ctggccgtca ccctgcccct ggcctttttc ggcgagggcg gcagtgggct
    11581 gcggctcggt ctggtgctgg ccgtgttttg catcgtgcag attctcgacg gttacttcat
    11641 cactccgcgc atccaaggca agaagacggg actcagcgat ccggcggtca tcttctcgtt
    11701 gctcttctgg ggcgtggtct tcaaaggcat cctcggcgta ctgctcgcca tccccctcag
    11761 cgcattcatc gtggtgctct ggcgactact caaaaccaag tacatccggg agctgatcta
    11821 acgcgggcgg agggcagcgc aagggttttg tctgtaaatt cgtccggcgg gtcacggaat
    11881 ctcataaacc taaaagccaa ttcgccgtac ccgcgcagtc cgatcccgta ttcccgaccc
    11941 atgaacgaac acactcccgc tcgccgtcca aaccgaccgg acacacgctt cccccgacag
    12001 gccggccagc gacttcgcaa ccgggacaaa ttgacggtgc gctacagcga aaactgcggg
    12061 atcttgagcg tcgtgccgcc ctttatcgcg gagaggtgcg cgtagatgcc cgagaccgtc
    12121 gtgttcccgg aataacgcgc cgcacatcga ccgcattttc tgttttggtt ccgcgcccct
    12181 tcccgttaca atgaaacgca catgtcccta ccgcttctag aggtcgagaa tctggccgtg
    12241 gtcttccgtc aggagggcgt cgagaccgtg cccgtgcgtt cagtctcgtt cacgatccgc
    12301 gcgggcgagc gtgtcgcgat cgtgggtgag agcggctgcg gcaagagcct caccgcgatg
    12361 tccctcacgc gtcttccgcc cacagaccgc gcccggcttt ccggacgcgt gcgattcgac
    12421 ggcaccgaca ccctcgccct gaatcacgcc gggttggcgc gcgtgcgcgg acgcggtatc
    12481 gcttacgtct ttcaggatcc ctcgggcagc ctaaacccgg tgatgcgcgt gcgtgaccaa
    12541 atcgccgagt gtctgccgga tctgcgccgc gccgaccgcg ccgatgcggt agttcggctg
    12601 ttgacccaaa ccggactggc cgatccgctc cgcgcggccc gtgcgtatcc ttgcgaactc
    12661 agcggcgggc agcaacagcg cgtgatgctg gccatggcgc tcgcggcccg ccccaaactg
    12721 ctggtggcgg atgagccgac caccgcgctg gacgtgacca cgcagcgtca agtgctcgat
    12781 ctgatcgaca cactcgccga cgcctgcggc atggcggtct tgctgatcac ccacaacctc
    12841 ggcatcgtgg ccggccgtat gtcgcaggtc catgtcatgt atgccggcca aatcatcgag
    12901 agcgggcccg tgacgcaggt gctgacccgg ccccgccacc cctacacccg cggactgctc
    12961 gccgcggtgc cactgctgga cggtccccgc gacgcgccgt tgcaggacat cccgggccgc
    13021 gtgccctcac ccgatgtctg gccaccgggg tgtgcctttg ctccgcgctg ccccgaggct
    13081 tctgaacgct gttccgcgca atctcccccg aagaccggag acgcgacaca cacctgccgc
    13141 tgctggctgg aggaggcccc atgctcttag acgtacgtga cctccatgtc ctctaccgca
    13201 cacccggtcg ccagcctgtt gcagcggtgc gcggtgtcac gttctccctc gacaccggcg
    13261 aatcactggg actggtcggg gagagcggct gcggcaaaag ttcgctcgcg cgcgcgttga
    13321 tcggcatcca gccgcccgaa gcgggaacga tccgtttcga aggacaggat atcgcccgcc
    13381 tgacgccccc cgcgttcgcg cggtaccggc gtgcggttca aatgattttt caggacgcgg
    13441 cgggctcgct caatccgcgc atgaccgtgc ggcagacgct ggaagaggtg ttgcgcgtcc
    13501 acgcgatgcg gcctgcggcc cgtattcccg accgcatccg cgaactgctg gaccgcgtcg
    13561 gcctgccgga gaccgtgctc aatgccttcc cgcgtgagat cagcggtggc caatgccagc
    13621 gcgtcagctt cgcgcgctgc ctcgcgctcg agccgcgttt gatcctggcc gacgaaccgg
    13681 tcagcgcgct ggacgtctcg gtacaggcgc gtatcctgaa tcgcctgcgg tcactgcaga
    13741 aagaactgaa tctggcgatc attctgatct cgcacgatct ggccgtggtc cgcaacatct
    13801 gcgaccgcgt ggtgatcatg cgtgccggcg agttcgtcga atgcggcacc gcgaacgagg
    13861 tgctcgacac gccgcaacat ccctacaccc gcgaactgct ggctgcggtg cccgacatcg
    13921 cccgttccct gcggtcaaag gtccctccgc aacctgtaac ctgaaacccg tattcctccc
    13981 gtactatgcc ccctcccctc atcctcggtt ccgcctcacc gcgccgcagt aaaatcctgc
    14041 aagctctggg ctgcgtcttt acggtttcga ccgttgacac gaaagaactt cacgacgact
    14101 tcgatccaat ccgtgcggtg accttcaacg cgctggagaa acaccgtgcc tgccgtttgc
    14161 gccatcccga ctcagccctc atcaccgcag acacgctggt ctggtttgaa ggacgtctca
    14221 tcggcaagcc ggccgacttc gacgaggccg ccgagctttt gcgctctttc tccgggcgca
    14281 cgcagatcgt ctacaccggt gtggcgctca gtctgccggg tcaggagccg gaactacgcg
    14341 tcgaagcctc ttccgtgcgt tttcgagagc tgactgccgc agtgatcgcc gactacctgc
    14401 atcggacacg gccgcttgac cgtgcgggcg cctatgacat cgacgagaac ggcgacctgt
    14461 tgattgccga ggtctgtggc tcgtacacaa acgtgatggg gctaccggag gcacccgtgc
    14521 gcgactggct ccgggcccac gggctcatat gaccgcgatg gcctcgatct cgaccttcgc
    14581 gcatttcggc aggcgcgtca cctgtactgt ggagcgcgcc ggggtgtggc tgccgaacat
    14641 ctcggcatag accttgttca tagccgggaa gtcccccata tcggtcagga acaccgtggt
    14701 cttgaccacc ttgtcgagcc ccgatcccgc agcctccaag accgccctca ggttcagcaa
    14761 cacctgcatg gtctgttccg cggccccggc catctcgatc atgcctgagg caggattgac
    14821 cgccagttgc ccggaacaga agaggaaccc gttggcttcc accgcctgcg catagggtcc
    14881 gatcactgcc ggggctgttt ccgtgctgat ctttctcatc gctcatccct ccctgatcgc
    14941 gtcgtttacg gtgcggtcac gcccagccgc aacaccggca ggatctcctg gtgcagcgtg
    15001 gtgtcgagat cgaaaaacag atatcccggg cacccggcgc ggcgcaccgc ccggatctga
    15061 tcaatcacct gcgcggcgtc gagccgactt tccgccgcag tgacgccgat accgggcagc
    15121 acgcgcatcg tttgtttgcg caggcgcgtt tgctgcgaca gccactcgtt gaagcgggag
    15181 agatcctcgg tgtagttcat gggcatgaca taatccacca gcccgatatt gatccacgac
    15241 tcccaatcct gacccaccgc atcgaggcag gagggatatt tgccgaagac cgccgcagtc
    15301 aagagcttgc ccggcgcgtc gcgccggagc gtccgccgcg acgcctgcac cagatccgta
    15361 acccgttccg cacgccaccg gatgaaggac gcgcgcaacg ccccgctctt cacatcctcg
    15421 ggccagtccg cggcgctcct tcccgtggcc ttctcaaaac gggttcggac ggtggggccc
    15481 aaactcgacg cgtaatccgg atagcgcacg aaatccagat gcacaccgtc ggtcttgtac
    15541 gccgtggcca tctcccgcac agccttgagc agatactcgc gcactgcggg cacggccgga
    15601 tcaagccacg ggcgcggcgt tccgtcggga tgggtcagca gccaaccccg tttagcgaaa
    15661 attccgatcc ggtcattcgt ggccccttcg gttgagaagc agagcagcca ggcatgaacc
    15721 cgcaacccca gcggtttcgc cgctgccaag cacgcggcca actggtcgcc ctgctcatcg
    15781 aacacacgag aacgcggcag caccccgctg gcgtaatgcg cgaatcccgc acccgccaca
    15841 ttcacaaaaa gatcggagat ccccgcatct ttgagcagac ggcaggtccg gctccaatcc
    15901 ccggggtaga gtcccatccc cgagtgttcc cacacgccgc ggaactcgcc tttgcgaaca
    15961 ggctgcgccg caccgtacgc ctcatacatc cgggcccgca gatcctgcgc cgccagccac
    16021 gcctcatacc ctttgcctgc ggccagcgcg cgcacggtct ccgcctcact gcggagcgcg
    16081 ccccgggccg cgttcagagc ccgcgtgcga cgggccgggt ccgtgagccg ctcagcgttc
    16141 attcgcaggc cttcggcccc cccggccccg ccgatccccc gcgcccgatc cagcacacgc
    16201 gccgcagcag gccgccagag cgacggatcc cgcgcagccg ccagagccag aagcagacgc
    16261 cccttggcgt ccgcatcgcc atcggccagc aacacatggg tcatccagta tccgttcggc
    16321 gacatcagcc aagcggcttc cccggtctgc cggccctgac ggtcgtgcca ccacgccaac
    16381 acctgcgaac ggcctgccac aggctgaacc cgaaagaggt ttctggaggt ctggaggatc
    16441 cgctccggcg tgccgggcgg acgctgcgca gagaaacgca tcatggacca gcggccgtca
    16501 gttgaaccct tctggtaccc cacggtctcc atgcccagaa gagccgcgag ttccgctgaa
    16561 gaggagtagc atacgatcat gctgccgccg cgacctgcaa aggccttcag cgcggcgagt
    16621 tgcatggcat tcggctgaga aagatagacc agcaccgcaa cgcgggcgga cgcgagggaa
    16681 cgggacagat cgcggtcatc ggtcagcacc gcctccacgc cgacctcgcg gtaccaacgc
    16741 tccacatgcc gcgcaaggga tgccgcaaaa cggcgttccc cctcactgac ggattggtag
    16801 ccgtgaatca ccgccaccgg tgcggccccg gccgtgaggg cggccatcac catccacatc
    16861 gtcagccgtc gtgtcatcct cccccccaag ccctcatccc acaccgtgct cagcaccgtt
    16921 cctcaaaacg aatccgggcg gatcgcccgt gcgcatccaa accctcgacc tccgcaaacg
    16981 tttcgatcgt gcggcgcgtt tcgatcagat cctcccgcgt gaactcgaca aagctgtgcc
    17041 gccgccggaa atcatccgcc gtgaggccgt tgaacatgcg cgccgcgccg cccgtgggca
    17101 gcacatgact gggaccggcc acaaaatcac ccgcagcctc gggagtccac gggccgacga
    17161 aaacagcacc ggccgcgcgc acctgattca gccgcagccg cgcgtcgcgc gtcatgatct
    17221 cgaagtgctc cggcgcaaac cggttcacca attccatacc ctgatccaga tccggcacca
    17281 ctaccgccag aataccgccg acggcaagca cgcgctcgat acaagcggca cgggaaagga
    17341 gccgcgtctg cgccgtgagc gcgtcgcgca ccgcccgagc cagaaccaac gaatccgtca
    17401 ccagcaacgc cttctcgtgc ccgctgccgt gctcggcctg agacagcaaa tccgcagcaa
    17461 cccattcggg cgcggcggag tcatccgcga ggatcgcgat ttcactcggt cccgccacct
    17521 gatcaagggc cacaatgccg tacacctggc gtttggccgc agtcacatac gcattgccag
    17581 ggccgacgat cttctgcacc ggccggaccg tggccgtacc gaaggccatc aggccgatcg
    17641 actggatccc gcccacccgg tagacctcgg tggcgcccgc gacccggaga gcgtgaagca
    17701 acacggggtt gacccgtttg tcgcggccgc acggcgtaca ggccacaatc tcccgcacgc
    17761 cagccgtgcg ggccagcgtg acggtcatca ccgccgtgga ggcgagcggc gcagtcccgc
    17821 cgggcacata aacaccgaca cggcccatgg gcgaaaagaa ctcgccagta aagccgccac
    17881 gcggcaacgg catgtgccac gcctcgcgca gactggcctc ggcataccgc cggacccgcg
    17941 tatgagcgtc gttcaccgcg cgccggaccc gtgcgggcac ctcgcgggtt gcggccttga
    18001 gttccgcatc cgtcacgcgc atctcggaga cccggagcgc ggcaccgtca aaccgccggg
    18061 ccgcggccag cacggcccgg ttgccgtgcg cgcgcacctc cgccagcact gcggctgccg
    18121 cagtctccgc ctctggggaa aaggccggac gcgcgaggaa ccgttcgacc ttaggcgtcg
    18181 gacgggttag ggaccattgg gctatcgtcg cacgcatgct cgggcatcct cgctctccgc
    18241 ctttcgtaac cggcctcatt ggtcgcccgc aggcttcttc tcctctttct tcgcgtcttt
    18301 cttcgcgtct ttcttcgcct cgtccttggc cggctcgtcc ttagccggct cgnnnnnnnn
    18361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccggctc gtccttagcc ggctcgacga
    18481 acgtcacgcc gaaagtgggc accgggcgtc cgagtttctc ggccatgcgc ttgctgatca
    18541 tcgcaaaggc atcctcgaag gccttctcgc ggctggcctc tttggcgagg tcctcatcct
    18601 tcatcatctt cttccgccta gactctttgt ccatctcttc gagttgcttc tcgttgatcc
    18661 gctcctccag acgcgccttt ctggtctcgg ccttttcgcg gttcggaaaa tctgcgccca
    18721 tctttttggc ggagcgcggc ttgatcttgt ttcccgggaa ggggagtttg ttctccttgt
    18781 tggcatccca ctgctttttg ctctcggccg ccgcgaccgt atacgccgcc gcctcctctc
    18841 tgatctcctt ctggagcgcg gtgtactctt cgcggttcag gatctcaaac ccggtctgac
    18901 cgcgcatatc cgtgatctga accagactga ccatctcggc gcgcgcgagg cccaccgcca
    18961 tcagtaccat tgccatctgc atcaacccgt tccgcatccg acacctcctt ctttctttgc
    19021 tgcgggcgaa aggcccgcac ccgatcattt cgattgctgt cccggcaccc actcttctga
    19081 aaaacgggga cggcgctgcg ccaccgccag acgcggggcc gcagtcacag ccgaacgcgc
    19141 gggcgtttcc gcacgccgcg cgggcgcggg ccgcggcttc tccggcttcc ggggctcagg
    19201 cttgggttcg ggcttggccg cagccggttc tttgggctgc gccgcattgg cgggaaccgc
    19261 ggcgggcgac ggctccgaac ggcgcggacg cgcatccccg cgcacggccg tgcccccttc
    19321 ccccgcgcgc tcgcgttggg ccccggcggg acgacgcgag ttggcagact ccgacagcga
    19381 cgcacgccgc gaccggcccg ccggtttagg cggcaacttg gccagcagcg gatcatcggg
    19441 cgccaagacc gtgcacttca gttcctcgcc gatgtacttc tcgatctcgg ggataatgaa
    19501 cgactcatcc tcatcggcga acgagatggc cactccgcta gcccccgcgc gaccggtccg
    19561 gccgatgcgg tgcacataat cctccggctc gtaaggaaag tcgaagttga caacgaaccc
    19621 gatgtcgtcc acgtgcaggc cgcgtcccgc gacatccgtg gccaccacga tccgcacccg
    19681 gccctcgcgg aaatcctcca gcacgcgcag acggcggttc tggttgacgt cgccgctgag
    19741 catctcgcaa cggatgccgc ggcgggtgag gctgtccgcc acatcctcgg tcgtgttgcg
    19801 gcggttgcag aaaatcagca tacgggaatc ggggtactgc tggatgtggt tgtagagcac
    19861 ggtgaacttc tcattgaccg tcaccacata caccacctgc ttgaccgcgg cggtggtgac
    19921 ctgctcggac tccacctcca ctttgaccgg cgcctccatc cactggagcg cgaggcgcat
    19981 gacctcttcc gtgagcgtgg cactgtacag catggtgctg cgccgattct tggggggcag
    20041 ccgcgcgata atcgaacgca cgtcagggat gaaccccatg tcgagcatcc ggtcagcctc
    20101 atcgatcaca agggtgtcca ccttcgacag atcaatcaca cgcgaccgca cgaaatcgag
    20161 cagacggccc ggtgtggcca ccagcaggtc caccggcgcg tccgaaacct cgcgtttctg
    20221 ccgatcgtaa tccatgccgc cgtagaccgc cagcgagcgc agcccgcaat acttacccaa
    20281 cgcctcagcg tctttgcaga tctggatcac caactcgcgg gtcggcgcga tcaccagagc
    20341 gcggggcgcg ccccccttct gctgccgcga ttcgggagaa cggagatagc gggtcaggat
    20401 cgcaaccaga aaggctgcgg ttttgccggt gccggtctgg gccttacccg ccacattctt
    20461 gccggccagc gcatgctcca agctcagcgc ctggatcggc gtgcagtact ggaagccgag
    20521 atccgcgacc gcgtgcagga tctcgccggg cagatcgaag tcatgaaacc gcttcttccc
    20581 ttcggccggt tccaccgaaa aactctccgg cgaccacgag gcgtgttcgg cgatgcgcgc
    20641 cgcccgttcg gaatccccca ccgcaccgcc cggacgccgt gtgttcggat ccgagcgttc
    20701 gccgccgccg cggccgcgcc cgcgacgcgg ctctttggat ggcggacggg caacggaacg
    20761 cgcggccgcc gcgaccgcgg gcggccccga ttcgcggcgc ggccgcgttt cagaaccgcg
    20821 tccggacgac tcccggcgcg gtttcccgcg accggcatcc gccttgcgtg cgccctcgcg
    20881 gcccgaagcg ccccgggacg accggccagc cgaaccctct ttggacgcct gaggtttggc
    20941 gcgcccgccc tgcggacggc tccgccgccc gttgcgcgac ggttcccgct tgggattctt
    21001 cgcgccccgg ccagtcttgg ccgccggttt tttctgtgac tgttgctttt gaccaagtag
    21061 tttgtcggct atttttttaa taaatccgag tgacatatgc gtaaacctgc tatcagaaaa
    21121 aaatgcggac gcctgcgaga gacgcccgca cattcaagta ggatgtgacg aaaataagac
    21181 taacgcttcg agaactggaa gcgcttgcgg gcgccaggct gacccggctt tttgcgctcc
    21241 ttcatgcggg cgtcgcgtgt caggcagccc gccttcttga gcacatcgcg caatgtggcg
    21301 tctgacgcca ccaacgcacg ggagagaccg tgacggatcg cgccggactg gccgctgatg
    21361 cctccgccct tggccgaaac gaacacgtca tatttcccct gcatcccggt cagctccaac
    21421 ggctgactca gatattggcg cagggcctcg ctgggaatgt attggtcgat cgcaacgtca
    21481 ttcacgaacc atttgccatc gccgggaacc agacgcacgc gcgcgatcgc ggttttccgg
    21541 cggcctgttc ccgctgcaat ttctttcgcc atctgaaacc ttcttctctt gtccggccct
    21601 gagccgggat cctataactc tgtccgccgc ctgatcagaa cgtcgccgca accggcatct
    21661 gcgcctcgtg cgggtgttcc gcgccgcggt acaccttgac gcgggtcatc atcttccggc
    21721 tgagcgtgtt tttcggcagc atgccccaca ccgcctcttt gatcacgcgg tcagggtgtt
    21781 tttccaacat ggtctttgcc gtgaaacgct tgagcccccc acgccaacct gaataccgct
    21841 ggtattcctt ctgctcttcc ttccgaccgg tcagcaggac tttttccgca ttgaccacaa
    21901 tcacgaaatc gcccgtgtcc acactgggat caaaggtcgg acgattcttt ccgcgcaggg
    21961 tgttggcgat attcacggct agccgcccga gcggtttacc cgtggcgtcc accagatgcc
    22021 aactgcggtt cggacctgga tctttcggtt ttgtcgtctt cgtcatgttg aacctgtttt
    22081 tccttcttcg cgaaaaatgg tctataaggt tatcccatcc cgcgcccggt gtcaacgcgc
    22141 aacttccggt ttttacaccc gcaaaaccga ggcccccagc gcggccagca gggcccccat
    22201 cagcccctgt agaaacccgg cggcccggaa caggcccggg cgtccggtga gccagtcgat
    22261 ggcttggcgg agtttccacg ggtagagcag cagcaccatg cccttgatga tgcacaggta
    22321 ggccacggcc accaccacca gcctccaggg cgaggggtgg gtgcgggccg tgtgcagcag
    22381 atcataggga aagagcgtca gcagcccccc caacgcgcgg cacggcaaca ggttatcaag
    22441 ccagaaccag gtgagaggga tgcaaaccag taccgcaaag gggatgtagc gcttgaacgg
    22501 catgagcagg tcgagcccca tggtccagag cgcggctccg gcccagatcc acgcgagggt
    22561 actcagcacg atcccggccg cgcggttgcg cggaaaagcc cggaacaccc gtgcggccgg
    22621 tccgggacag aggacaatcg tcacgccgag cgacaggcag aaaaggccga gcagataaca
    22681 ccagttcgaa agggacatat aggaatctcc gtaaaatgag gatagtaaag catatcccgg
    22741 tgcaggaggg gaagcttgat ccgcgctcag tgacctccat cgcccggggc ccggattcgc
    22801 ccccccccga tctcgaagtc aaacataagc ggcagatgat ccgaaagtcg cacccggggg
    22861 atcttgaagc gcacgggggt gatgatggaa gggtcatagc agatgaaatc gagttcccgg
    22921 cagggagccc agcttgggaa agtcggcgcg tggcgcgtgt tcgcgctccg gagtccggtc
    22981 gcccggagga acatgcgcat ctcccaaacg ccggagaggg tgttgaagtc gccggccacc
    23041 agccggggct tgtcacaccc cgtgatcaga tcgtacaggt cgctaagctg gcgctgccgc
    23101 gcgcgtatac ccagcgccag atgcacaaga aagaaggtga cgttctccaa ctcgacctcg
    23161 atggccagac ttttgaaacc ggtttcgaaa tcgtgaaacc gggtgcgcag aatcggttcc
    23221 cgcaccaaca cggcgttcgc ctgtttgttg aacaccggca gacgccgccc catgccctcg
    23281 tccggatact tcacccgaca acagtggtag agcgagagat ccgccgccag ctcctcggcc
    23341 tgattccggt gccgcgcgcg gtaagatccg gcgtccacct ccaccaaccc cacaatatcc
    23401 gggttcatat ggcgcacgaa ccgcgtgatg ttcggaaagt gcgcggtagt ccgcaacagg
    23461 gtctccaccc acgcgaaccg ccgcaaccgg ccagtcccat actgaatgtt gtgcaacagc
    23521 aaccgcatac gtcctctact tgaccggcag cgaaggcgcc agagacttca gatcggtcca
    23581 aagcgtgccc tctccggcgt ggaggatccg gatacccgca tcggggttga gcttgtccac
    23641 aaaggtcaga tcggccagcg tggcgggatc cttgaaaacc cccgcgcgca actgctcgcc
    23701 cagcgccttc tcgttggccg tcaggtagtc ggcccggacc cgggcctcgc cctgcacccg
    23761 tgtgatctgc gcctgcaccg cagccttcag aaggttggtg gcggcaaccg caagcgcggt
    23821 gccggcctgg atgttcgcga cctcgctgcg ggtccgcgcc acggtcgtgg ccacgagttt
    23881 ctcggtctcc tgctcgacct gtttcttgaa ctgttcgatc atcgcggttt cggtgttgag
    23941 ctccgcctgt ttgcgggccg tgtcctgacg cgccttgttg gtcagatcct gttctttcgc
    24001 gagacttgaa tcctgaatcg gagtaaggat ctcgcccggc acctccacat ggcggatgat
    24061 cgcgttatgg ataacaatgt gcttctcgcc catcacgcgc gtgagttcag cggtcagttc
    24121 cttctggaaa agctggcggc cctcgccgtc gatgaaatcg cgcgccttgt agctggatcc
    24181 ccggatgcgc gagatggaga gcacctgcgg caggatgatc ttctcgacca cggcgggcag
    24241 atccccgtag agcatgtaga tacgcgagat gtcctccggg agcagctcga actcgaccgt
    24301 catatcaagc aggatctgaa aaccgtcccg cgaggggaac tccacaaagc ggttgatgcc
    24361 gaacgccacg gactccggca ccacagcctg ccgcattgcg gcgggtccat cggtatccgc
    24421 aagcagatcc tccgtttccg gtgcccgtct tcttcctccc ggcgcctcca tcgcgtacgc
    24481 tttcgactcc ctcggtgacg cggccgacac cttgcgcgac aaggccttac ccaaaatctc
    24541 ccgactcttg cccagcgcgg cggcaacctc gttttgggcc agcaggttgc tgttccgccc
    24601 aatatagtcg gcgcgcttct gctgctgctt ctgaatcgtg gtggcatgca gctcatccaa
    24661 tgcgctgctg gcattggaga tttggctctt ggtcagcacc acgctgccgg agcggccgtt
    24721 gatcgagacc tggttcatgc cgatctccag cacgtccacc tgataggcgc gcggattgac
    24781 atagtaaagc cccggctgga gcacgtcctt cataacgccc tgctgtccgg gccccgcgaa
    24841 ctcaccggtc ttcaccggct cgccgctctg cgacgtgacg atccccacat acccgatcgg
    24901 aatgctgacc gcgtccaact gggtgatctc gtacccttcg ggattcagac ggtaggtgcc
    24961 gggaccgagc acgtctttcc acacgccctt accctccttg tccgcagcca agatctcgcc
    25021 gggcggcaac tcccggccga ctttcgcggt caccaccccg accttgccga ccggaatgga
    25081 aaccagcggc acgatgcgcc acgagtgggt gactggatcg cggaagtggc ggccttcggg
    25141 cagcgggtcg cgctgcacgc ctttctcacc gggaccggcg aggatctggc cgggcggcag
    25201 cgggtgcccc gtctttctgg tgatgatcgc catatggccc gcaggcaccg ggatacggca
    25261 gaagagccac agccaggcgg cagcgaacag ggcgagggcc agcacaggaa agagagcgag
    25321 agagattttc ttcatggcgt gtcccctttc gcaggtttca ccgttccggc cgcaggtgcc
    25381 gcgggaccgc ctttcgcccc caccggcagg ccaaacaaac cctgaccgtc gtccgaggtc
    25441 aacaccccct gaatcttcgg cgcggttttc tcataaagtc tggcgcgcac gtaatcagcc
    25501 tcatcgcggt aaaccgcaac ctgctgcttg agcacatcgg cgttggcccg gttctgcgcc
    25561 tcgatcacgc ggcggtcggc ctcggcaagt tgaagcaacg cctcggcatc cgcacgcgcg
    25621 acttcgagct ggatctcggc tacggacagc tcggtacggg ccgcaagggt ctgctcgatc
    25681 cgcacctgtt cggccgcgat ggtctggcgg atgcgttcgg tctcggccgt gactttggcg
    25741 ctgttctgga tggccagcgc cttttcgcgc tccagatccg cttgcgaccg ggcctgcaca
    25801 atctgctgct cgaacttcag agactcttgg tttgccagtt cccgatcccg gatgatgccc
    25861 gcaacctgct gcggcgcgat gatatcgcgg atcagcaccg aattcagcga gacgccccag
    25921 ttggcgcaga tcccgcgcag atacttctcc aagctgtctt ggaaggcctg ccgcgactcg
    25981 cccacaatga actccgtggc gctcttcttg ctcccctcga tccggaagaa cccgcgcgcg
    26041 ctcggcagga tcagcttctg gcgaatgtcg tccatgtcgc cgacctcatg cgtcagcaac
    26101 gcgaccttgt cgatgttgag attgaactcc agcgtgccct cgactgtcac cggaaaaccg
    26161 tcgagcgtga ggaacatgat cgcctcctcc ccgctgaact cgaaccgctg gctctggatg
    26221 ttgacgatcg aaaccgcgta caggaagggg ttgaggcggt gcgtgccctc cttgagcacc
    26281 tcggactgca cgcccttgca tcccggcgcg gccagaaacc ccttctcgcg gaccacgtcc
    26341 gatccgtcta ttccgctcag gatgtcccga ccgcatagcg cggtgaccac cccgacgtgc
    26401 cccggcgcga tcttgatgtc gtcgaacaac cggacctcgt aggcataagg gttgatccgg
    26461 tgcttgccgg tgccgagcac gtcgcgcacg atacccttgt acccctcgtc cggcgcgagg
    26521 atctccccgc ccggcaggtc cttgccgtat ttgcgcacca gcaccccgaa cttgccggcc
    26581 gggatgtccg tgacccgaac gatctgccaa ccccacgtat agggattgcg gaagaaacgg
    26641 ccctcgggca gcacctcgag ttgaatgccc ttgtaccccg gctcatccgc gatgatccgg
    26701 tccaccggca gatttttacc ggttttccgg aacagcaccg ccatcttgcc attgcccggc
    26761 tcgatgcgcc accagaacca gaaccaaatc ggcccgagaa tgagcgccag catcaggagc
    26821 aacggcgcca gcgaaaggga ggaaagcgcg gcccggcgcg acggcgtacg acgcgacggc
    26881 gcggatcgtc gattctccag cggcggcggc atgatttcgg catcttcctg catggtgacc
    26941 tccgtttgtt gtgaacctga agaacccgtc ggcatactga acgtgagaga ccggacttca
    27001 gggcgaagag tctgtaaagc tgtagggttt cagtataaag gggcaaggca tggagcgtca
    27061 acgcaaggat ttagggaaag acgcatccgg gcgcatccct gctgttttga ctgcggtcaa
    27121 gcggcgggag tccgcaggag agaatcgggg gaagaaccgc actggacggc tgacaacaag
    27181 gacgaccccg atcccaatga cgatagcgat ttgaacgtgt acggcctccg gacggacggc
    27241 ttgtgtgcga gaagagacgg tctggaattg cacttgaaac atctggacag gcgcacgaac
    27301 ctctaagtat attatccacg caaagagcaa gaggaaccac aaatgacgac atcatcggcg
    27361 atcacccccc atgccgtggc ggcacacctc ggcaagcccc cggcgcagtt caccaaggcg
    27421 gatatcatcc gcttcataca ggacaggggt atccagatgg tgaacttcat gtatccggga
    27481 ggtgacggcc ggttgaagac gctcaacttc gtcatcaacg atttggccta cctcgacgcc
    27541 attctcacct gcggcgaacg tgtggacgga tccagtcttt tctccttcat cgaagccagc
    27601 gggagcgatc tgtatgtcgt gccccgtttc cggacagcct ttctcgatcc gttcaccgat
    27661 atcccgacgc tgaccctgct ctgctctttc ttcaacaagg aggggcggcc gctagagaac
    27721 tcgcctgact acacgctgca caaggcttgc aaagcgttca ccgacatcac cggcctggag
    27781 ttccaggcga tgggcgagtt ggaatactac gtggtggcag acgacgttgc gctctttcac
    27841 gctgtcgacg accagcgcgg ctaccatgaa tccgggccgt acgccaaatt caacgccttt
    27901 cgctcccact gtatgctgac gatcgcccag tgcggcggcc tcgtgaagta cggccacagc
    27961 gaggtgggca atttcaccca gaacggcaag atctacgaac agaacgaaat cgagtttctg
    28021 cccacctgcg ccgaggatgc cgcagatcaa ctcgtggtcg ccaagtggat catccgcaac
    28081 ctcagccgtc aacgcgggct caatgtcaca ttcgcgccca agatcactgt gggcaaggca
    28141 ggctcgggac tccacatcca catgcgtatc atgaaagacg ggcgcaaccg gatgctcgcc
    28201 gacggcgcgc tttccgatac cgcccgcaaa gtgatcgccg gcctgatgac actggcgccc
    28261 tccatcaccg ccttcggcaa caagaacccc acctcctact tccgcttggt cccgcaccag
    28321 gaggcgccca cgagcgtctg ctggggcgac cggaaccgtt ccgtgcttgt ccgcgtgccg
    28381 ctgggctgga ctgcggagaa cgacatgagc gccgtggcga accctctcga aaccgcctcg
    28441 gttcgggaca cctcggacaa acagacggtc gagatgcggt cgcccgacgg ttcggcggat
    28501 gtctaccagt tactggccag tctcgccgta gcggcccgcc atggctttga aatgcccgac
    28561 gctctggacg tcgccgcgaa aacctacgtc gatgtgaata tccataagac gggtaacgag
    28621 tcgattgccg catccctgag ccagcttccc ggctgctgcg tcgattcggc cgcctcgctt
    28681 gagcgtcaac gcgccgtctt cgaggctcac gacgtcttca gcccgggcat gatcgacggc
    28741 gtggtggcgg ccctccgtgc ctacgacgac aaaaacctcc gtgccgagct gatgaagtcg
    28801 cctgaacggc tcgcggagct tgtcgagcgg catttcaact gcggttagtc aaccgacgcg
    28861 ccccgtattt tcggtgccgt ttcatttgag gcaacctgaa gtcgccctcc ccgaaccttt
    28921 ttgattccgg tgtcatgaat ggtgaggcaa cgcgacggat ttttacggat tgacggtttt
    28981 caccctcctg cgcataatga ataccatcgt ggatgtccgt gtgttgtcgg gcatcgtgtg
    29041 gctgatcgtg gattgaacgt gatgaatgaa gagaacgaga aaccggtgaa aacgcgggag
    29101 gagcttgcgg cgattgcgta tctcgacaag ctgcgacgcg gcgtgggcga gacgatgccg
    29161 acggagctgc cgctcgcgtg tgtcgtaaag gtggtcgcct acaaggagat tccgaaggcg
    29221 aacgagaagt atgcgctggt gaccgttcgc ggtccgatcg ggatcacgtg gaagatgtgc
    29281 gtcaaccgcc actatcttaa aaagggcatg aacgcgctct tcgtctcaaa aggcgctgcg
    29341 atgcccgagg aggagcgttt ccgtaatccc gaagccgcaa aggtgaagct gcgcgtgttc
    29401 aagttcggcc cgggcatcaa ggaacgacgg cttctgccga tcgtcagccg gaacatctat
    29461 ctcttcaact gcgggctcct ctatccgctc gacgacttcc cggaattaca gggggtacgc
    29521 gcgggcatgg tctgtgccgt aaaactccga atcgaccgct tgaaagagct gcatgaacgt
    29581 gtcgccgcgc cgaagccaaa gcagaagaaa aagcccgcgc ccgcgccgaa ggcggaatcg
    29641 gacggcgatg atagcgtcct tgccaaagtg cgccgctatt acatccgggt ctggagggca
    29701 cgataaaaag cattgccctg tttttcacgc gaagccgcga agtttgtgtg gcggcaagac
    29761 ggtgccgcgc aacgtcagac accaggccct ttttcggcgg tccgggacgt cccgccgtac
    29821 ctccagcttg agcagatacg cgcgccagac ttttacatga catgctgttg cgagcgaggc
    29881 cgcatccggc gcgtaggtcc cgtcccgtat gtctcggtct gtgcggcggg cctgcctccc
    29941 gtcaccccga tcactcatac gtcagccacg ccgtcgccgt acaggcacgg tcggcggtca
    30001 cgcgcaacca atacgcgcgc gcatcatcca gcaccacacg ggtgggttgg cccggcgcca
    30061 cacgataggt atcgtacacc cgccacacac cggtaccggt gaggtcaagc tccagcgtga
    30121 ccgttacgat cccggccgcc gcatgcgaga gcgccagccg cttacggtcg aacccggtca
    30181 tcagataagg gtcggacggc acgcccgcag cggtgacgct gtccttccag gggccgcctt
    30241 cgccgcgcgc tttcccgaac gcccagagat cgtcgacact gccgacccaa accgccgcac
    30301 gtccatctcc ggaacggatg aggtgacgcg acgtagccgc gttctcccgg attccggtca
    30361 tgaccagcaa cccacggtac gagcagtaat cgtgcacctg cccgcaatgt gtcgcgacag
    30421 cccgcacctt gctgaaaccg cccgcatttt cggcgggcaa ctcgtagaag atgccgcaaa
    30481 cgttcagcag atcacgttcg gtgcagacct cgcggcacag acgcgtggcg ccgaaaagcc
    30541 caggcgcagc gtacgccgga tccgccttgg ggaaacgcca gcgccgcccc gcatcatcgg
    30601 taatgagcgc ggaggcggcc tcgaccacca gccccgtgcc gctcatcgga taatcggtac
    30661 gcgccacctt ccacgccgcg gcatcctcct tgcgcgtgag gttcagatcg gcgtccaact
    30721 cgtaatatcc ggtttcacgc acaccgtccg gaccagcggt gaccgccgcg agccccagca
    30781 cgcgcttatc cccgccgcgt gcgtacacga ttccgccgta cgcgcgggga tcccgcaatg
    30841 acgccagacc cgcgcaacgc gcgtccggcc ggtcggaacg gcgatccggg ttggcgtact
    30901 ggaaacaggc atagacgtcc gcagcgtcgc gctgcggcac gagccgcacc caggcacccc
    30961 gttcccgttc cgcgaaggtc agggccgtca gccgccccgg tgccgcctcg accgttcgca
    31021 cggctttcca gacgcccttg cccgcgcgat ccgtttcgag ggtgaagcgc accggctccg
    31081 cactgccgtg ggcgagcccg agcgagcgga ccgcataccc ggagaacaga tacggatcgc
    31141 tcggaacccc ggcggaaacg gactctccca gccacacggc accgcggccg atggccggac
    31201 caaacgcgtc caaacgctca ggattgacga accagagatt cgagtgcgac tgacccggac
    31261 cgcggatcgt gcctttggca cggcgcttgt tgagaaactc cgatttcgcg ctgtcatcgc
    31321 aagccagcac cagccaatca ccccagcggc agaaatccgc cacaaccttc agatagtttg
    31381 aaagcggcgc gatccccgcc gaatcgtgcg cggaaaaggt ttttgggaaa cgccagaaag
    31441 tgccgtgcat ggtcatcagg aacgacttgc caccgatttc gcggatgcgc ggccactcgg
    31501 tgttccatcc gtgtccgccg tcgtaactgt ggctgccttt gggcaaccgg taggtgtgcc
    31561 acgcaccgca gtccagcacg gtgagcaaca gcgaacggtg atcccaaccg atcgcccaga
    31621 ccggatccgt ttccgggttg gggttaccca aaatgccgcc ggggccggtg acgtcggtga
    31681 actgattgcg ccgcacgacc gtccacgccg cagccttgcc gtcccaaacg gcgagacatc
    31741 cggaaggggc cgagggatca acgaacgcct gccgcgcttg gtcaccgttg ttcgcataga
    31801 tcagcaggcc ctgacccgaa taaagccctt taccgtggta ccccggcaac ttggcgaagc
    31861 ggggattgtc accgggagtg cgcttcgcct tcgcgagctg ttcatcgcgc cacagctctg
    31921 tcacggccag tgtgcggaca tccacctcgt aaaagccctc ctccatactc gcgtagtaaa
    31981 ttttgccctc cggatcggtc aggtggcgcg cgttgccggt gggccgaccg tacatgacgc
    32041 tgtacgggat cgtccggacc gtgcgatccg cgccgatcgc gtacgggcca atgaaaagct
    32101 gattggattc gggatggatc atccggttgg cgggcgtgcc cccgatactc tcggacctca
    32161 cgaccgaaac cagatcgggc gtgatctcat agagcttgtc atccgaccct gtgggactat
    32221 ggggggaata ggtcagggtc cacaaccgtc cggcccacgg taccaccgca ccgatcccgc
    32281 actcacctcc gccattatac atggccagat gcgggtagat gccgcttaca aaagcgtact
    32341 gcccggtgac catcggtccc ccggacggcg cccgtacccc tgcaaaaatt gcgccagccg
    32401 ctcccgcgac cgcccatacg accgctgtcg aacaccacct cttccaacac ataaccgcca
    32461 cctttttcgt tgttgatcag cgcaccgcgc ccggaaaacg gcccgccttt cttgtcatcg
    32521 cccctccccc cttctcttgt cgtgtttcaa ggacaccgga ccggaccggt tcccgcggtg
    32581 ccgacgtcac cgagttcgac cgttacagtc tctaaagaat tcgccttgtg gaagacggct
    32641 ttcatcttac gcgtggccgg catacccgaa taggtgcccg tgcgcggacc caccgtcagg
    32701 gcgccgtcct tgaaggcaca tgtcgtcagc gcgcccttgc ccgaacggta ttcgagggag
    32761 accccgtcat cttcatagag atcgcccttg ccgtcggccc ccggccacac atgcagctcg
    32821 accgtctcgt cccaaccttt gtccacatgc gctttcttct cccacatcgg gatcaccgca
    32881 cccgcgcgca caaacaggct gccgccccac tccggcgtca ccttgaccgc gcgtttgcaa
    32941 gggccggtca ccaactcacc cgttttccag tcgtgccaca acccttcggg aatctggatc
    33001 tccttcgtga
//
LOCUS       DAOSRB010000006        31857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1560_length_31857_cov_7.702283, whole genome shotgun sequence.
ACCESSION   DAOSRB010000006 DAOSRB010000000
VERSION     DAOSRB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 31857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31857
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1560_length_31857_cov_7.702283"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PLZ74_00745"
     CDS             complement(<1..1168)
                     /locus_tag="PLZ74_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOR96882.1"
                     /translation="MRSMVAVFLGCLALGAQAFTVSTIYSDNMMLQRGKPIKVTGSGA
                     PNETVEVTFDGETVKAAANAHGRWLATLAPREACKVPKTLTVTGGGKTVTFKNVLVGD
                     VWVCSGQSNMHWNIAWGGCYHQEDVKAEAETNAPYFRGIDLRRGSLLFPAAEVRLDGC
                     SYKKWVKMGKRPGEVSALGFMFGLKLSKACPDVPMAIISLAQGGSRIEPWIPLDGYDG
                     IKGLEEEAAAAELYDFGKPQGRAVLNGVLKDISGWAKGFAALSTNDFPYAPQPMLPRP
                     LKGHAGLYNHMMAPLETFPFKGVIWYQGESNEDDAKYGIKQRALITSWRRRFDTDMPF
                     YFVQLPNFGEKNKPGDFRMRKYSAIRILQAESLDLPLTGMAVTTDTADDNQLHPK"
     gene            complement(1188..2300)
                     /locus_tag="PLZ74_00750"
     CDS             complement(1188..2300)
                     /locus_tag="PLZ74_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96883.1"
                     /translation="MKSRILMVAILAAVSKGMAARTSRVFGDNMVLQSGRPIPVWGTG
                     EVDETVSVAFGGNTASCRVGADGKWQVVLKAESPAVAPRELTITAASGAQTFKNVRVG
                     DVYIIMGDRHVGWPFWSVGAEIKTDLDRYADTNLCYFISPIQRRASYPLDDLEPPTPH
                     AGFWRSVVNKDRLSSFAYFYARERAVKTKMPVGVIVVTGGAYDRCDARMFMRPEAYAA
                     SQNDKGNPQAYDALNPTSEKGLAALKEAAARIQTWADDALALPQGEYPASNMPELPNL
                     EKYGQVTTHYNYQMSPFAKTPIKGAVFYAGYGWGYDPKEAPLAEELKTDMRRVWGDDI
                     AFTIVPVPEKTVMEGDCPSECNKPLEVVRNLDASNK"
     gene            complement(2323..6378)
                     /locus_tag="PLZ74_00755"
     CDS             complement(2323..6378)
                     /locus_tag="PLZ74_00755"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOR96884.1"
                     /translation="MMNKIMAALAVAFGVFSALAAETVELKAKPTVTPVELNFGDTVK
                     FTLLNGETRTIRLVDTSAQIIEIPTVEGLVFMFDVTLEVDGHRVKIDRYTATQQAFYE
                     PIVINGMRIWADMALSVYDVIPMRYPGTGNQRQRPWKDMRLALQDATIEDCPVKLSKW
                     FDYRHPSIFIGDCYHGGDCWMGSFGAGVAHGGLDVNQREGDPLWAPMDFDDQGYFQSL
                     ATGSNNNRWRGWRTWPDGSIWAFQCHHVITQYVAQGMSVKQGTHLFDGAGVWVGWYNH
                     NHYEFKVANGKMPDWNAGGRNSADGRGGRALEHGQPEVYHLDPWIYFRQIFRNEREKT
                     GYVYPEMAPPGPAKTAEAVVFELTKGGSDDVYWDFGDGAFGRGAKVTHRYAKPGLYAA
                     RAVIVREDGSLDEVIRHLTVNGAASLLPSLIVQSDDHRFRPWPTGTQYAFGYDLVNYR
                     PGGFEIKAPIGQRNLPTESRTFRFANAGGGVLPPIAAALSEPCDWIVLQPTADGVTFT
                     VDPMKAKDGVNEAAIVLKGEGLLNGEVTVPVRLAKVKCGYYTGSFRLDSNAHTCFYAT
                     PGFWCSHRLSGSKRYYKMSGNRPESDAIARFIPEPMAAGTYRVRVDGDAPWAAGSKCW
                     VDVKDAAGLHRVRFEPAKSWTVGTFRFAAGGGNYVEFRTQDSVGSVAVNHLAFDRLPD
                     TPPVAKAPYVPETIELKAKPTVTPVEMNCGDVVKFTLLNGATRTLTLIGTSARMAACT
                     TTETILGFDATLEIDGHRVTFQRNTCEPEAFYEPFVVNGLRIWPDMALKIFDVMPMRQ
                     ACEGNGRRRPWKDLRLALQDATIEDCPVKLSKWLEYPRLAPSVNDGRVGGMCWMGSFE
                     GHHIHGGLDIRQQPDKTALSAPLDLDDQAPLPERTKHSREGRWRGIRKWDDGSVWAFE
                     CHHIVTQSVPAHVSVKQGTTFLTGGKPHPGHHMHTHFEFRVANNGMPDWDASTYARDG
                     GAGTRALEHGQPAVYHLDPWIYFRQIFRNERERTGYEYPELAPIGPAKTGEAVAFEVL
                     RGCGTATNRAEVCWDFGDGAFGRGEKVTHRYAKPGIYAARAVFARPDGTFDEVVRHLT
                     VDGTRIAVPDLVVKSDEPAFFPHPAGVPQAFAYGRVNLRPGQFAFITDEKRPSAETRT
                     LRFANAGGGVLPKVTATLKTPCDWLTLASVAEGVVLTADGTKAPAGVSEAVIVLAGDN
                     LLNGAVEVPVRFDVGAHTLIKRDRLNLDASGVGFYATPGGWLGLAGGAFTSGGRNRPG
                     EVARFTPQMLEAGEYTVCIPLNTPWPMGHQCNVRVRDAGGVQTVRFDPAKSMTLGAFR
                     FAAGGGNYIEFESAGATGEVVVRKVRLIPNR"
     gene            complement(6381..10223)
                     /locus_tag="PLZ74_00760"
     CDS             complement(6381..10223)
                     /locus_tag="PLZ74_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96885.1"
                     /translation="MNVEKVVATVAIAAGVCFAANAAEWKTLRDLPVSAVGNAAYLTS
                     MTERPWWNKAWTRRTPILVSSAAKASCGKIVVDAVVDFGEQVNPAEVRLVTPWETEVP
                     CWVEAKEGTKLELYFETDLRIEENRPFLVYWGNPAAKAPDLWPTMTMRVNDEEVRIAN
                     GVLDVTFDNLHRTSGLLKKIRVLASQTPNELLDRASGFAWDGFSLRSGAAEPWSKAKV
                     VADNPFKKQIRFDSKKVTLDFTLYANQPRVDYAYTLKPGNEQANISISWACGDGTGED
                     DLFYPGLAGKVLSLRASLDHATDCIPNPHYEGLGKYLAEGWYAIRDRKQHEVVGMIFD
                     RPTLASFDYSGAGQYAGERSVMTFRHQTEKDRPVGGSGALVALMGGVDDVRNEYLRYC
                     KAPIISVGKTEAYREIAVRRPRIDRDFCADYNIGKGAGAGWESGEPLEGGEWCSNIVD
                     RVRSLGGNIVRIGGYGWQDLAITKELYDRIAKVWALPTSRSRNRKMPEWQEGQYTGEK
                     FRQHCAAAHAKGVAVSLWSGFVPGVGFDNDMLWDKDAQELDTELQCLYADLGADIIYN
                     AMACREGAHIPTDVIKGRGTSWWLWDDPRDFFKTEDIRTEAIKKFYRDCKRRHPDTPV
                     VVMNSECSIIERETMMTDQVGAFDSVYCEMLPLCDNPRTLPHTKMAVKRLRALYDNEA
                     GRTVHAHFYYYGHDCVQRILQVELPYVWGINGFTFENLTYENFGKEQPNITADAYRFL
                     EYTKLGDKVARMAPVKDLAVFRDNRAYVDDVINHRRGKPYAYSTQQDGRANAIAEIRN
                     CVYDIVIDPFFTAKALAKYRAVYVPEDLSFGEDRAKELIGYVQAGGGAIVEGRTVEGN
                     GLSKLGLTDGKVTDLGKGKIVWLKDVLTDRLARRDAKAFAQAKELMASVGCLAPFTLP
                     SKTLDANLQISEEGMFLGVFNDGKEEDKGRATLNLSHLPNFSHLSNPLYVLDVKRGVR
                     FRYTNGFEVVVGPEQCGYYLIGDEKFTALPETSGGAWFGASASHVGEPGRGCKMPVLD
                     RSEAFPRVHALEYVRGSEKKPTMIRRSMLAAFDIKQVTPDAYDPSLFAAAIRKATYLH
                     LCANAKGADRAFADCPEELKAMLKRGGAILFDRTRPGPNAQKFLKEIGVFDPYPSAKT
                     KIGDGKSVWDETFPSNHLLSAFNRVKEGYFGNDFIRRLGRDGGRYNLAFGQWDAETQL
                     TPFRAALNKDYTHLIIQEHVLGAGKVAFNENARSFNDWYESKMYGDALLSWLIGMPVK
                     DYKDKAERYNGGPGKAVE"
     gene            complement(10290..11882)
                     /locus_tag="PLZ74_00765"
     CDS             complement(10290..11882)
                     /locus_tag="PLZ74_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOR96886.1"
                     /translation="MAMNVKPGAVFVPCLLAAGVLLAAEPFCEEAKGWKRVPAVSDEF
                     DGKALDKSKWDDWCASFPGRVAAGMRGEPCVNGFRFAPDNVSVEDGELVLTARLLTDE
                     ERKSHHQEHLRHGPYATSIVKSRKKQVYGYFEIRAKTMKACVSNAFWLYDPHSDDPRV
                     KYTAGDVSEEIDIFEVTGRPDSRGRNPDCSRKYYNTFHLYCTPYLEGVINAKKINPPD
                     RAFVTKVDFDFCDDYHVYGLLWTPEKLVWYLDGKPVKERKNDGLFHRPLHVTLDSEVF
                     VSWFGVPDPKDLPAKFRIDYVRVWECPVSGNVALRPRMKIEKDSYDWFERHNEAMACA
                     RARKPEIVFVGDSITHFWAGGRTLGGEAAHPRWKQKFGAYRTLNLGYGWDRTGNVLWR
                     LDHGEMDGLDPKLVVLHIGGNNFSATKNYPGNNAEETAEGVLAVLDKIRTKVPQARIV
                     TMAIFPFGEKPDAPHRVKARAANKILAVEVPKRPNTVFLDITDKQVGADGIYPKSRAN
                     DFVHPTDAGYDIWYEALKPYLP"
     gene            complement(11919..13424)
                     /locus_tag="PLZ74_00770"
     CDS             complement(11919..13424)
                     /locus_tag="PLZ74_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96887.1"
                     /translation="MTISKNGLLAIVVGSMLSGVAATGDSGRVEVTLDRSPDRVETPL
                     NPPLVRIGTLVAREKPDPSDDRWQIGCEVLDRDFANFDEYKGFLVPLGIRNVRFQGGW
                     AKCERTKGVYDFSWLDAQVDWVLAHGLNPLIETSYGNPIYNDVRADLGGGFPSGEKAL
                     AAWDAWVNALTRHFKGRVRDWGMWNEPDNNPQHTPERVAAFNVRTARIVKRNIPDARV
                     AGLVFNHPDTNRFDRALKAMGPDTALFWRFVYHSYEYNPESSYGDVAHCQALVKKYAP
                     HAVLWQGENGAPSETVYTLALLGRPWSEYSQAKYNLRRMLGDLGRSIPSSVFTICDYN
                     HVGRELGTFGLLRANKSHEVIGVKRAFYAVQNVATLFDSRTKLVARRNYSKDGTIEIW
                     EYRRDGKRLYVFWDHKDGKSVPPSESFELRPFVMDVPKGAEALETPVWIDLLTGRIYE
                     FPKERVLRHSDGITYTDIPLYDSPCVLTERLVVDAAMLAPTQSNVRTRASH"
     gene            complement(13447..15933)
                     /locus_tag="PLZ74_00775"
     CDS             complement(13447..15933)
                     /locus_tag="PLZ74_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96888.1"
                     /translation="MMGIRMKSGIVLMGAVFFTFSAAFGGVWEKYAGNPVLGNAKLGT
                     CFDVNVVTNGPAPYTMYFSWRPKKAIALVRSDDALSWTQEPEICLEADPTSGWEDDLN
                     RSCTVFKDGKWHMWYTGQAKGGSKIGYATSADGMKFVRVRKDPVMVPENDFEKKSVMN
                     PYVRWDAQRGVWRMWYAAGETYEPNVLCYAESKDGVDWKKWEKNPMFGKGAAESWDRD
                     RVGGCETHPLPDGRWAMFYIGYSDIDTARIGCAISPDGVSDWKRIAQNPIIAPDLCTW
                     DASACYKPSVLRDEKNNRWMLWYNGRNGWSEYVGMALHEGLDLEAPSSKVPDTKALLS
                     TYVRGFNKFDNECVTNAIPNSDAERFLSENVPLFACPDKDIERTYYFRWWTFRKHLRH
                     GTGVWTVSEFLPKVGWSGKDNTIVCPAGHHFLEGRWLRDPKYMTDLARFWLTDKECTH
                     RWQYSSWLYTGVCKFAAVAGKEALADELLDAAVAYFERWEKGFDRRKWDEEGKGSIRM
                     GGDGKGRFLSARTNEGTEFTLTPSGYRPLFNAAMWSEAHAISEVAARLGRSDLATRYA
                     EKAAVIRRGLEEICWSPEIAFFVAADTNRTRCAVRELHGYAPWYFGVPTDKKADWSQL
                     SDPQGFAAKYGLTFPERRAKGFVLAYSGHECQWNGPSWPFATSIALTALINDLHARPS
                     IPNPQRSTFNFCLWQYAAQQKLIKGIAVPWIDENANPDKPDWISRTILHKRSKEKVKF
                     ERGKDYNHSTFCDLVISGLVGIIPEGAKGFTVNPLAPAAWDYFMLERLNYRGHEVSVV
                     WRRGHGGLTVTVDGKVAARRDDLGRLAIRF"
     gene            complement(15978..17864)
                     /locus_tag="PLZ74_00780"
     CDS             complement(15978..17864)
                     /locus_tag="PLZ74_00780"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOR96889.1"
                     /translation="MNKNDLTNAAHRICNADTFGTGHKTIRLAVFAVSVLTAAAPCVQ
                     AAEVTLDYTYIDGRTRREKMPLDSENRLVVKADHIGPGVKTLDVAPDFATAKKGEDGY
                     FLMPDGLYGTFRADNGKFYPAARNYHPLFIMKTPRVNFMAHATGLAYSYETHVVAKNG
                     GYRLFNRYNFNGDPPYEDLVIRFRFGAADVSWCAFAREYRDDQLKRGACRPINERMKD
                     FPALAYAATNIEVRLRLGWKPVPAKVRDQTPFTEPPVKVAITFDRCRDIMREFKRQGI
                     PEAEFCLVGWNKGGHDGAYPQLFPVEPKMGGETKLRALLKDAKTMGFQTVAHNNYSDA
                     YLIADCFDEEFTLKKKDGLPKTAGTWGGGFQYLTCPQRMCERFLVKDLEMMRDLGFFG
                     LHYTDVIASQPLRKCYDPRHAINEADCIKWYARIQSETKKRLGGFASEGIFDYCVGDL
                     DYCLYAYFYKLVPGELNKMVDRHVPLAQIIYNGIVLINPFCGTVNYTIKDNVKRLKLV
                     EFGGRPIFYFYTNFVEGFRWMGDEDFTCATDAELTASVAKIKQGYDEFCRLSDLQFVF
                     MDEHEYLASNVTCTTYANGTKVIVNHGTADFPYKGEVVPAGDWRRFDSTEGNPVASLL
                     RSDR"
     gene            complement(17949..19547)
                     /locus_tag="PLZ74_00785"
     CDS             complement(17949..19547)
                     /locus_tag="PLZ74_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOR96890.1"
                     /translation="MKKTWIIGCSMAAFAAMANVKPANVFSSNMVLQRDKVVPVWGTA
                     DAGEEVKVSFAGQSVAAKADADGRWEVKLQPMPASKENRTMVIEGKNKIEFKDILVGD
                     VWLCSGQSNMEMSFNWGIYDGEAFKKEAAEKFDPIRCVKVSKIHSTWPKADVKLDHPW
                     ARTTANGGIANWTATGYFFARRLVQELDIPIGILDDNWSGCRIEPFIPLSSFKNYSAT
                     NGAYAKQVKSFLELQEKTDPRTEAGRKVFAKYAAEMKAFLAAVEAAVAEGKLPEADMP
                     ALPSVSATSQYNAMIAPIVRFPIAGAIWYQGCSNGQEGESYTEKTEMLVKGWREAWGY
                     EFPFYWVQLASFTAKTDDPKGGNGYALIRDAQRLALKRLEKTGMAVTIDVGNDKDIHP
                     KAKVFVGERLAAWALKGAYGRDVVPSGPLFREAEGLGTAEVTLSFDYVGGGLTAGEKD
                     NLSNAPVKLLPDAKLVGFAAEVEGGQFVWADAKIAGDKVVVTAGGKTIRNVRYGYRAN
                     ATGLANLYNREALPASPFATENKW"
     gene            complement(19593..21251)
                     /locus_tag="PLZ74_00790"
     CDS             complement(19593..21251)
                     /locus_tag="PLZ74_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96891.1"
                     /translation="MKTLTIVAAVCAPAVLLAGLSVSPVFMDNMILQRDKPIPVWGRG
                     EAGKEVTVDFAGARARATVDADGRWAATLPALGVSNDDRTMTVTMGEKQGFFARLFGC
                     APEGERVTFANVLVGDVWLCSGQSNMEYSFCWWPYSMDLSKAFCTNTPNFRYLRVARH
                     KAPVPRETPEIQGKWSVLTGQVWFTASCVGCFFAQTVMEKTGKKIPIGLIDSSWGGMN
                     IEPYIDPTCLRDDDPKALKAIKDRYDENANPATAKGRMKWDAYLARQKAFVADIEAAV
                     VDGRPPAGDPAEVPVSHTYWNGCYQAMIHPFAKYPVKGALWYQGCGNYPDNENYQYLL
                     EGLVRGWREAWGYTFPFYWVQLSSFDNKGVVDDAPAGGLGFTRVREGMRRAMATIPHG
                     GMCVTFDLGDPKDIHPREKLHVGQRLAQWALKNEYGFDDIVPSGPNYRAMKIEGDRIR
                     LSFDYVGSGLAVGYKNNFDDRDVKLMPEAKLSGFAIAGEDRTWFFADAVIDGDTVVVS
                     SKDVKKPVAVRYAYRTSPIGANLYNRELLPASPFCTDDWEREKK"
     gene            complement(21353..22834)
                     /locus_tag="PLZ74_00795"
     CDS             complement(21353..22834)
                     /locus_tag="PLZ74_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96892.1"
                     /translation="MKMCIAVMLGLAVTVTFADGTKAYARPGSDGGPLYTARGDAPRI
                     AKMITEAQAAGRREVVIPYRNDAANDDKWIIEETVLIPSGMKVILDGARLVQADGVCC
                     NLFANEHAWTDDRCTAKAEDRDIAIEGRNGATLDGGNWNGLGERSKPPELAGKRLVHN
                     CFVYFHNVKGFKVSGLKCRHQRYWATCFSFCEQGVIRDLRFEADISWVSEDGRDHRPN
                     RLPGKYVNLWVKNGDGVNLRKGCHDIVVENITGWTEDDVVALTNLCGEEMKDIVEGKS
                     TDIHHITVRNIRAGTWLWFNQVRLLCCDGAKVHDVVIDGVHDEYDPSWMNWQRNASAV
                     QINDSAEEYYRKRNCAMGEMRDIVIRNVTSGAGFPIRLFCPMQNVTIENLHLLPGAKG
                     ALAVQCDAEFDNVTVSGITADETVRLFSFLNFHQAEGAITVRNARVPEAEHLIRNSGT
                     CQMTLENVEIGKLNGEREIKSCGGHWIGDKVPLYQDLKPGTRP"
     gene            complement(22850..24328)
                     /locus_tag="PLZ74_00800"
     CDS             complement(22850..24328)
                     /locus_tag="PLZ74_00800"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOR96893.1"
                     /translation="MKRQMMLMTGICLAGAAWAQEKAPLPPAVQAQMAAEFAKAKLVT
                     EGLSDYERPWTLSAYVPNDRIVWEVGERPEAVFEVRGADGRLLTKEQLTNVTMSVSWY
                     RTHETTVEDIVLSQEGNPVRRILTLDKPGVVAVTAKLRGIPEPLNGRYPNVRDLRQAR
                     VDEKGKRHSLTLSYRVGALFNPLEIKPGKPIPEGFDAKWREFLEEDAKYSNVPKHFRR
                     RGTNDAENVWVYAGVLDSPGGEVHFDWGVPKVVREGKGKLPIRVFLQAYGTGFHFAET
                     WISGELTLGINCHSISNRASNAYWDEARKRHGNFGFPTNENAKVETCYHINMLRRDIR
                     ALRWAMSHPGWDGTNLYVHGDSQGGFQTFALSGLFPQITKAWSMCPWYVDVGGSQKHW
                     RPLWQPGPDFADPVHHITRTKAQNITYTFGICDSAGPADGMMAMFNGIPDTTAFAEIT
                     IIQNRGHDAGHTSKDLFPHYVFTKRFGKVTGAYHLPTGETKR"
     gene            complement(24493..26592)
                     /locus_tag="PLZ74_00805"
     CDS             complement(24493..26592)
                     /locus_tag="PLZ74_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 32"
                     /protein_id="HOR96894.1"
                     /translation="MNVEKVLTLSVLAAAVCALSGFAADGAPTAKIKPCAGSEIRQFA
                     PGERLFFDRHFVISKKVAPQLVGKSFVYASINGISFRVTQSGEILALTPPAEFTDKGG
                     KPSQESELKKLGFAEVNDIPVSQLFNTSDKEKVRVWRKQVKEGERYELDYWCVIAGFD
                     PKPAVTVKKDVPRETLYNGIVLETDPRDRTDMRKYGRYPLPVPYLDNPPAVIPVDAGR
                     QLFVDDFLIASTTMVRTWHKAQKDPKNPVLRPETPLERGALGPIKTPMAAPFSGGVWF
                     DATDRLYKCWYCAGWFDGTAYAYSKDGYNWERPVLKVDGKTNRIVKPETRDGKNCHRD
                     SAAVILDPDATDGNRFKAFIWSRPQGGEIFTSANGTDWEWRAACAWSGDRSTLFYNPF
                     RKVWCYSLRDFWSARSRQLAESKDFVAGVAYTNRVCWQRADCCDRRDMRWVYCEEGNY
                     EKEPPEPSLYNLDCVAYESLMLGAYAIMSGADNSEFQRRGSPKLTELHLGFSRDGYHF
                     SRPEDRSPFIPASRTYGAWDRAYLHSNAAICLVKGDELLFYYTGFAGQIGEWKDHPVG
                     GLYTLAQMGIARLRRDGFASMDAGRCGGELVTRPIRFTRGDRLFVNANAAGGELKVEI
                     LDEDGQALAASKPFTANAVKSEILGGLSSYAGKTIRLRFTARDTQLYAFWFSDASGRS
                     RGYLAGGSPEADGLKDE"
     gene            complement(26685..28610)
                     /locus_tag="PLZ74_00810"
     CDS             complement(26685..28610)
                     /locus_tag="PLZ74_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96895.1"
                     /translation="MKTKAFRATVIVAATILGAAVQAAEWYVDKDSTADTHDGGQATP
                     FLTIQEGLDAASANDIVHVAKGSYLISAPLTFKADGVSLVGDDGVAANTVIDAQQLTN
                     CVTSTYFNSHFHNLTFTNGRCEVTSAYGGGGLCLTKGGAVISNCIVTSCAVIGTGEIG
                     IAGGGIKANGGDLIDSLVEHCCISNTVTEAYTNATSCNGGGVQGLSTGRRSIIRNCSA
                     VCTRGVARYNFMQGGGAHGGSWYDCAFISNRLVNPLEIYNCRGGGLFTALTTVSNCYF
                     EGNYITGKGSGAFLDRVYACGNNSFIGNIGSAQAGAAMHGIAAFDCYFEGNDLPGGGN
                     ECGSGGLYLDQNGVAHGCAFRNNSGILGSAMQSHGGTVVSNCLFDSNLCTYAGKYGGG
                     VVYYGNQRLSTFLSDCVFINNSSTSDGAAFYAFGTSNATVRSCLFVSNTVKHVLYILE
                     WNVAQSGIAAGEGACGRVENCTIADNTLLGSSYALKGSQYDLFQRNNLIYNNCLEDTT
                     TSRALEDWYAGTNVVRHCFIDSRASNAASFDKDGNVIGSDPMFRDSAQGDYRLTRRSA
                     CYNAGTNLAWMTEGALDMGNGFRETHPATITAFGETFPVGVKLEKIDPCARVEGGRVD
                     IGCFELFRPLVFTMSVK"
     gene            complement(28627..30708)
                     /locus_tag="PLZ74_00815"
     CDS             complement(28627..30708)
                     /locus_tag="PLZ74_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96896.1"
                     /translation="MRNVGLVVVLSVCALAVRARTVYVDLNTSAVMPDGMTWETAYPT
                     IGEAVSNLLTNNYESGSAIYIAPGHYALSEPIPAIPGSNPVTFYGVTNVLLKANPGFA
                     EQVIIDGQNATNAFEAIQGYAKGSQFHGLTIVNCRKDVFAETGLELYGGSAINARNSD
                     AVISNCIIRNCWSGGFREGGTAGCIYGGAVAVANGSVVDTLFEDNGITNACASAESQT
                     LTGLRFRGGAICGGGYKEFRGNIFRRNSIVDPTGKWGDLDGGAMHGVGTLTADCRFET
                     NRVWSSRTDKAAGRGGAVFTEGSGKVFSNLVFVGNSSVSQPVCVNASAPALFTHCTFV
                     GNRTERYGASLTTSLKLGAGNEAVIRHCLFKDNYSPGGVADGSGTAVHAHLNDTQGGY
                     RGLFIHDCAFVGNVNKDFGMAALSTWSGNGGAYVSLSNCYFEANLSATNSGTHALLAI
                     NSGLNFSLVDCSFIGNQSGNNGNQPLVKQSGRENEVGSTTNSVVRNCFFYGNASSRVL
                     DISNPVADIGQEFPGTRVESCTIAGNRLSGTSEAILAQTGKKFYVCNTAVWGNYKAGT
                     ADNYNLNASMAAQTSHSYIGPQAIAVDAFTTNVLSGNITEIDPGFADPAKGDFRLKGR
                     SPLKNAGKAQPWMAGANDLSNQAVVWDETLTQGGCDVVRVPALPRIFPTVPDIGCFES
                     WAPLGTGMYVK"
     gene            30877..>31857
                     /locus_tag="PLZ74_00820"
     CDS             30877..>31857
                     /locus_tag="PLZ74_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96897.1"
                     /translation="MKDGVRLVCALAMPAWILHAEQPCENWLSPYTGADATGEKVVGL
                     WTFADLKDASGKGRDAVLHGTVLVPDARWGSCLESFCGWPVEDKRHAAVVANHTSLSP
                     RGAFTLEMWIKPKPEFVDYPEAFLIDKKYVAHTDYQWTLGAADKSGQRRAYLRLGFGD
                     DSEVYSSEPSVFATNIWTHLAVTYDGAGTVRFFRDGTAQGTVTRTGRGRIAPGGHPLS
                     IGDRIGSYYHGFPGYLTQVRLCQGALEFRPVTLALTSDRSVFVRMEKTPALRFKLANA
                     QREAAHNISVLCTVEGREAARHQIPELASGESRTIEVPVDTALRPGSYTLR"
BASE COUNT         5968 a        10074 c         9337 g         6478 t
ORIGIN      
        1 tcttgggatg taactggttg tcgtccgccg tgtcagtcgt gacggccatg ccggtcagcg
       61 gcagatccag cgattcggcc tggagaatac ggattgcgga gtactttcgc atcctgaaat
      121 caccgggctt gttcttctcg ccgaagttag gaagttgaac gaagtaaaac ggcatgtccg
      181 tgtcgaaacg cctgcgccag gaggtgatga gcgcgcgctg cttgatgccg tatttcgcat
      241 cgtcctcgtt ggactcaccc tgataccaga tcacgccctt gaacgggaat gtctccagtg
      301 gcgccatcat gtggttgtag agtcccgcgt gtcctttgag aggacgcggc agcatcggct
      361 gcggcgcata cgggaagtcg ttcgttgaaa gcgccgcaaa gccctttgcc caaccggaga
      421 tgtccttgag taccccattc agcaccgcgc gtccctgcgg cttgccgaag tcgtaaagtt
      481 ccgccgcagc ggcctcttcc tcaaggcctt tgattccatc atagccgtct agcgggatcc
      541 acggttcgat gcgactgccg ccttgagcga gcgagatgat cgccatcggc acgtcaggac
      601 aagccttcga gagcttaagc ccgaacatga agccgagcgc cgagacctcg ccaggacgct
      661 tgcccatttt cacccacttc ttgtaactgc aaccgtcgag gcgtacctca gccgcaggaa
      721 agaggagtga tccgcgcctc aggtcgatcc cgcggaaata cggtgcgttc gtctcggcct
      781 cggccttgac atcctcctga tggtagcatc cgccccaggc gatgttccag tgcatgttcg
      841 actgccccga gcagacccac acgtcaccga cgagaacgtt cttgaaagtg acagtcttgc
      901 cgccgcccgt gacggtcaac gtcttcggca ccttacacgc ctcacgcggc gcgagtgtcg
      961 cgagccatcg tccgtgcgcg ttcgccgccg ccttcaccgt ctcgccgtca aacgtgacct
     1021 ccacggtctc gttcggcgcg cccgaccccg tcaccttgat cggcttgccg cgctggagca
     1081 tcatgttgtc cgaatagatc gttgacaccg taaacgcctg cgcaccgagc gcgaggcacc
     1141 ccaaaaacac cgcaaccatt gacctcatcg tcttcctcga tctcctgtca tttgttcgac
     1201 gcgtccagat tgcggacgac ttccagcggc ttgttgcatt ctgagggaca atccccttcc
     1261 atcacggtct tctccggtac cggaacgatg gtgaacgcga tgtcgtcgcc ccagacacgg
     1321 cgcatgtcgg ttttcagttc ttcggcgagc ggcgcttctt tcgggtcgta tccccatccg
     1381 tagcccgcat agaacaccgc gcccttgatc ggcgtcttgg cgaatggcga catctggtag
     1441 ttgtaatgcg tagtgacttg tccatacttc tccagattcg gcaactcagg catgttggaa
     1501 gcgggatatt caccctgtgg aagcgcgagc gcgtcatcgg cccacgtttg aatgcgggcg
     1561 gccgcctcct tgagcgcggc gagccccttc tcggatgtcg ggttgagcgc gtcatatgcc
     1621 tgcggattgc ccttgtcgtt ctgactggcc gcataggcct cggggcgcat gaacatacgc
     1681 gcatcacaac gatcgtacgc cccgccggtc acaacgatga cgccaacggg catcttcgtc
     1741 ttcacggcgc gttcgcgcgc atagaagtag gcgaaagagc taagccgatc cttgttgacg
     1801 acactgcgcc agaagccggc atgcggcgtc ggcggttcga ggtcatcgag cggatacgac
     1861 gcgcgccgct ggatgggcga aatgaagtag cagaggtttg tgtcggcgta gcgatcgaga
     1921 tccgtcttga tctccgcgcc gaccgaccag aacggccagc caacatgtcg gtcgcccata
     1981 atgatataga catcgccgac gcgcacattt ttgaacgtct gcgcacccga tgccgcggtg
     2041 atcgtcagtt cgcgcggtgc caccgcaggg ctctcggcct tgaggacgac ctgccatttg
     2101 ccatccgcac cgacccggca tgatgcggtg ttgccgccga acgcgacgga tacggtctcg
     2161 tcgacctcgc ccgtgcccca cacgggtatc ggacgaccgc tttgaaggac catgttatcg
     2221 ccgaagacac gcgatgtcct agccgccatt cccttactga ccgcagccaa tatcgccacc
     2281 atcagtattc tgcttttcat attccttgtc ctgttctccc ctttaacgat tgggaatcag
     2341 acgcaccttg cgcacgacga cttcacccgt ggccccggca ctttcgaact cgatgtagtt
     2401 gccgccgcct gcggcgaaac ggaaggcccc gagcgtcatt gacttcgccg gatcgaaccg
     2461 caccgtctgc acacccccgg catcccgtac gcgaacgttg cattgatggc ccattggcca
     2521 gggcgtgttg agcggtatgc acaccgtgta ctcgcccgcc tccagcattt gcggtgtgaa
     2581 gcgcgcgact tcacccggac gattgcgtcc gccggacgtg aatgcaccgc ccgcaagacc
     2641 aagccatccc cccggcgtcg catagaagcc gacaccggac gcatcgaggt tgaggcggtc
     2701 acgtttgatt aaggtgtgcg ccccgacatc gaaacgcacc ggaacctcaa ccgctccgtt
     2761 cagcaggttg tcgccggcga ggacgatcac cgcctccgaa acccctgcgg gcgctttcgt
     2821 gccgtccgcc gtcaggacga caccctccgc caccgacgcc aacgtcagcc agtcgcacgg
     2881 cgttttcaac gtcgccgtca ccttcggcag cacgccaccg cccgcgttcg caaaacgcaa
     2941 tgtgcgcgtc tcggcactgg gccgcttctc gtccgttatg aaagcgaact gaccagggcg
     3001 cagattgacg cgtccgtatg caaacgcctg tggaactccc gccggatgcg ggaagaaagc
     3061 gggttcgtcg gacttcacaa cgagatcggg aaccgcaatc cttgtcccgt ccaccgtcag
     3121 gtggcggacg acctcgtcga acgtgccgtc aggacgtgca aacaccgccc gcgccgcgta
     3181 aatccccggt ttcgcatagc ggtgcgtcac cttttctccg cggccgaagg ctccgtcacc
     3241 gaaatcccag cagacctccg cgcgattcgt cgcggtgcca catcccctca acacttcaaa
     3301 cgccaccgcc tcgccggtct tcgcgggacc gatcggggcc aactcgggat attcatagcc
     3361 cgtccgctcg cgttcgttgc ggaagatctg gcggaaataa atccacggat caaggtgata
     3421 cacagcgggc tggccgtgtt cgagcgcgcg cgtccccgct ccgccgtcac gcgcataggt
     3481 gctcgcgtcc cagtcgggca ttccgttgtt ggcgacacgg aactcgaaat gcgtgtgcat
     3541 atggtggccg ggatgcggct tgccgcctgt caggaacgtc gttccctgct tgacgctcac
     3601 atgcgcgggg acggactgcg tcacgatgtg atggcactcg aacgcccaga ccgatccatc
     3661 gtcccatttc cggatgccac gccagcggcc ctcgcgcgaa tgtttcgtcc tttccggcaa
     3721 cggtgcctga tcgtccagat caagcggtgc cgaaagcgcc gtcttgtccg gctgctgacg
     3781 gatgtcgaga ccgccatgga tgtgatgccc ctcgaaggaa cccatccagc acatgccgcc
     3841 gacacgccca tcgttgacac tcggcgccag acgcggatac tcgagccact tcgagagttt
     3901 gaccggacag tcttcgatcg tcgcatcctg cagggcaagg cgcagatcct tccacggacg
     3961 ccgccggccg ttgccttcgc atgcctgccg catcggcatg acgtcgaaaa tcttaagcgc
     4021 catatcgggc cagatacgca gtccgttcac gacgaacggc tcatagaacg cctcgggctc
     4081 gcatgtgttg cgttggaatg tcacgcggtg tccgtcgatc tccagcgtcg catcgaagcc
     4141 caggatcgtc tccgtggtcg tacatgcggc catgcgcgcc gaggtgccaa tgagcgtgag
     4201 tgtgcgtgtc gcaccattga ggagcgtgaa tttcactaca tcgccgcagt tcatttcaac
     4261 gggcgtcacc gtcggtttcg ccttcagctc gatcgtttca ggaacgtacg gcgccttggc
     4321 gaccggcggg gtgtcgggca ggcgatcaaa cgcgagatgg ttcacggcga cgctgccgac
     4381 gctgtcttgc gtgcggaact cgacgtaatt gccgccgccc gccgcaaacc ggaaagtgcc
     4441 gaccgtccag gacttcgcgg gctcgaagcg gacgcgatga aggccggccg catccttgac
     4501 atccacccaa catttgctgc cggccgccca gggcgcatca ccgtccacgc gtacacgata
     4561 ggtgcccgct gccatcggtt cggggatgaa acgcgcgatg gcgtccgatt cgggacggtt
     4621 gccggacatc ttgtaatacc gcttgcttcc gctcaatcgg tgcgaacacc aaaaaccggg
     4681 cgtagcgtaa aagcacgtat gcgcattgct atcgagacgg aaacttccgg tgtagtaacc
     4741 gcacttcact ttagcgagcc gtaccggcac ggtgacctca ccgttcagga gtccttcacc
     4801 cttgagcacg atcgccgcct cgttgacgcc gtccttcgcc ttcatcggat cgaccgtgaa
     4861 cgtgacgccg tccgcagtcg gctggaggac gatccagtca cacggctcgg aaagtgccgc
     4921 ggcgataggg ggcaacacgc cgccgccagc gttcgcgaag cggaacgtgc gcgattccgt
     4981 cggaagattg cgttgtccga tcggtgcctt gatttcgaaa ccgcccgggc ggtagttgac
     5041 gagatcgtaa ccgaaagcgt attgcgttcc cgtcggccag gggcggaagc gatggtcgtc
     5101 cgactggacg atcagcgacg gcagcagact tgccgcaccg ttcacagtca gatggcggat
     5161 cacctcatcg agtgagccgt cttcacgcac gatgactgcg cgtgccgcat agaggccggg
     5221 cttcgcatat cggtgcgtca ctttcgcgcc gcgtccgaac gcgccgtcgc caaagtccca
     5281 atagacatca tccgatccgc ccttcgtcag ttcgaaaacg accgcctcgg ccgtcttcgc
     5341 tggaccgggc ggcgccatct caggatagac gtagcccgtc ttttcgcgtt cgttacggaa
     5401 gatctggcgg aagtagatcc acggatcaag atggtagacc tcgggctgcc cgtgttcgag
     5461 cgcacggccg ccgcgtccat ccgcgctatt ccttccgccc gcattccaat ccggcatctt
     5521 gccgttggcg accttgaact cgtagtggtt gtggttatac cagccgaccc agacgccggc
     5581 accgtcgaaa agatgcgtcc cctgcttgac gctcataccc tgcgcgacgt attgcgtgat
     5641 gacatggtga cactggaacg cccagatcga accgtccggc caggtgcgcc agccgcgcca
     5701 acggttgttg ttgctgcctg tggcgagcga ctggaagtag ccctgatcgt cgaagtccat
     5761 cggtgcccag agcgggtcgc cctcgcgctg gttcacgtcg aggccgccat gcgcaacgcc
     5821 agcaccgaag gatcccatcc agcaatcgcc gccgtgatag cagtcgccga tgaagatcga
     5881 ggggtgccgg tagtcgaacc acttcgagag tttgaccgga caatcctcga ttgtcgcatc
     5941 ctgcagggca aggcgcatat ccttccacgg acgctgccgc tggttgccgg tgccgggata
     6001 acgcatcggg atcacatcgt agacactgag cgccatatcc gcccagatac gcatgccgtt
     6061 gatgacaatc ggctcgtaga acgcctgttg cgtcgcagta tagcggtcga tcttgacgcg
     6121 gtggccatcg acctcgagcg tcacgtcgaa catgaaaacg agcccttcca cagtcgggat
     6181 ctcgatgatc tgcgcggagg tgtcgacaag ccggatcgtc cgcgtctctc cgttcaagag
     6241 cgtgaacttc acggtgtcac cgaagttgag ttcaaccggc gtcaccgtcg gcttcgcctt
     6301 gagttcgacc gtctccgccg cgagggcgga gaaaacccca aaggcgaccg caagggccgc
     6361 catgattttg ttcatcattt tcactccacc gctttcccgg gtccgccatt gtagcgttcg
     6421 gccttgtcct tatagtcctt gaccggcatc ccgatgagcc acgacaggag cgcgtcgcca
     6481 tacatcttcg actcgtacca gtcgttgaac gagcgcgcgt tctcgttgaa ggcaaccttg
     6541 cctgcgccga gcacgtgctc ctgaatgata aggtgcgtgt aatccttgtt gagcgcggcg
     6601 cggaatggcg tgagttgtgt ctccgcatcc cactggccga acgccagatt gtaacggccg
     6661 ccgtccctgc cgagccgacg gatgaaatcg ttgccgaaat aaccctcctt aacacggttg
     6721 aaggccgaga ggaggtggtt ggacggaaag gtctcgtccc atacggattt cccgtcgccg
     6781 atcttggtct tggcggacgg ataggggtcg aaaacgccga tctctttcag gaacttctgc
     6841 gcgttcggtc ctggacgcgt acgatcgaaa aggatcgctc cgccgcgctt gagcatcgcc
     6901 ttcagttcct ccgggcagtc tgcgaacgcc cggtccgcgc ctttggcatt ggcgcagagg
     6961 tgcaggtacg tcgccttgcg gatggctgcg gcgaacagcg agggatcgta ggcatcgggt
     7021 gtgacctgct tgatgtcgaa cgccgccagc atcgatctgc ggatcatcgt cggcttcttc
     7081 tccgaaccgc gcacatactc cagcgcatgg acgcgcggga acgcttcgga acggtcgagc
     7141 accggcatct tgcagcccct cccaggttcg ccgacatgtg aggcggaagc gccaaaccat
     7201 gctccgccgg acgtctccgg cagcgctgtg aacttctcat cgccgatgag gtagtagccg
     7261 cactgctccg gtcccaccac aacctcgaag ccattcgtat agcggaaacg cacgccgcgc
     7321 ttcacgtcga gaacgtaaag cgggtttgag aggtgcgaga agtttggaag gtgtgagagg
     7381 tttagggtcg ccctcccctt gtcttcctcc ttgccatcgt tgaagacgcc gaggaacatg
     7441 ccttcttcgg aaatctgaag gttggcatcg agcgtcttgg acggaagcgt gaacggcgcg
     7501 aggcagccga cagacgccat cagctccttt gcctgcgcga acgccttcgc gtcgcgacgc
     7561 gcaaggcggt cggtgagaac gtctttgagc cagacgattt tgcccttgcc gagatccgtc
     7621 accttcccgt ccgtaagccc gagcttcgac agaccgttac cctcgacggt ccggccctcg
     7681 acaatggcac cgccgccggc ctgcacgtac ccgatcagct ccttcgcgcg gtcttcgcca
     7741 aaggaaaggt cttcgggcac gtagacggcg cggtatttcg cgagcgcctt ggcggtgaaa
     7801 aacggatcga tcacgatgtc atagacgcag ttgcggatct ccgcgatggc gttggcgcgt
     7861 ccgtcctgct gcgtactgta ggcatacggc ttgccgcggc ggtggttgat gacgtcgtca
     7921 acgtaagcgc ggttgtcgcg gaagacggcg agatccttca cgggcgccat ccttgcgacc
     7981 ttgtcgccga gcttggtgta ttctaggaag cggtaggcgt ccgccgtgat attcggctgc
     8041 tccttaccga agttctcgta ggtgaggttc tcgaaggtga agccgttgat gccccagacg
     8101 tagggaagct cgacctgcag gatgcgttgc acgcaatcgt gtccgtagta gtagaaatga
     8161 gcgtggacgg tccgtcccgc ctcgttgtcg taaagcgccc gcaggcgttt caccgccatc
     8221 ttcgtatgcg gcagagtgcg cggattgtca cagagcggga gcatctcaca atagacggag
     8281 tcgaacgcgc cgacctgatc cgtcatcatg gtctcgcgct cgatgatcga gcattcggag
     8341 ttcatgacga cgaccggcgt gtccggatga cgccgcttgc aatcgcggta gaactttttg
     8401 atagcctcgg tgcggatatc ctcggtctta aagaagtcgc gcggatcgtc ccagagccac
     8461 cacgaggtac cgcgtccctt tatgacgtcc gttggaatat gcgcgccctc gcggcacgcc
     8521 atggcgttgt atatgatgtc cgccccgagg tcggcgtaaa ggcactgaag ctcggtatcg
     8581 agctcctgcg catccttgtc ccagagcatg tcattgtcga agccgacgcc ggggacgaag
     8641 ccggaccaca ggctgaccgc cacgcccttc gcatgcgcgg ccgcgcaatg ttggcggaac
     8701 ttctcaccgg tgtactgacc ctcctgccac tcgggcatct tccgattcct ggagcgcgag
     8761 gtcgggaggg cccacacctt cgcgatccgg tcgtaaagtt ccttcgtgat cgccagatcc
     8821 tgccacccgt agccgccgat gcgcacgatg ttgccgccga gcgaacggac gcggtcgacg
     8881 atgttcgaac accactcgcc accttcgagc ggctcgccgg actcccatcc cgcgcctgcg
     8941 cccttgccga tgttatagtc cgcacagaag tcgcggtcaa tccgcggacg ccgcaccgcg
     9001 atttcgcggt atgcctcggt tttgccgacg gagatgatcg gcgctttaca gtagcgcagg
     9061 tactcattcc gcacatcgtc caccccgccc atcagcgcga cgagcgcacc cgaaccgccg
     9121 acgggcctgt ccttttcggt ttggtggcgg aaggtcatga ccgagcgctc gcccgcgtac
     9181 tgtcccgcgc cgctgtaatc gaaggatgcg agagtggggc ggtcgaagat catgccaacg
     9241 acctcatgct gcttgcggtc acggatcgcg taccagccct ccgccagata cttccccagc
     9301 ccctcgtagt gggggttggg aatgcagtcc gtcgcatggt cgagcgaagc gcggagcgac
     9361 agcaccttgc ccgcgaggcc gggatagaaa agatcgtcct cccccgtccc gtcgccacag
     9421 gcccatgaga tgctgatgtt cgcctgttca ttgcctggct tgagcgtgta ggcgtagtcc
     9481 acacgcggtt gattggcgta aagcgtgaag tccagcgtaa ccttcttgct gtcgaagcgg
     9541 atctgcttct tgaagggatt gtccgcaacg accttggcct tcgaccacgg ttcggcggcg
     9601 ccggaacgga gcgaaaaacc gtcccaagca aaaccggagg cgcggtcgag gagttcattc
     9661 ggcgtctggg acgccagaac gcggattttc ttcagaagac cgctggtgcg gtgaaggtta
     9721 tcgaaggtga cgtcaaggac gccgttcgcg atgcgcacct cttcgtcatt cacccgcatc
     9781 gtcatcgtcg gccacaagtc cggcgccttt gccgcgggat tgccccagta gacgaggaag
     9841 gggcggttct cctcgatgcg caggtcggtc tcgaagtaga gttcgagttt cgtcccttcc
     9901 ttcgcctcga cccaacacgg aacctcggtt tcccagggcg tgaccaaacg cacctcggcg
     9961 gggttcacct gttccccgaa gtcgacgaca gcgtccacca cgatcttgcc gcacgacgct
    10021 tttgccgcgg acgacaccag aatcggtgtc cgccgcgtcc acgccttgtt ccaccacggc
    10081 cgctccgtca tcgacgtcag atacgccgca ttccctaccg ccgaaaccgg caggtcccga
    10141 agcgtcttcc attctgcggc attcgccgca aagcacacac ccgcggcaat cgccacggtc
    10201 gccacgacct tttcgacatt catcgaataa tgttaccatt ctccatcccg gtgcacaatc
    10261 ctcgccgggg taaaaaatgg cggcaccgtt tacggcaaat agggtttgag cgcctcgtac
    10321 cagatgtcgt agcccgcgtc cgtaggatga acgaagtcgt tggcgcgaga cttgggatag
    10381 atcccgtctg caccgacctg cttgtccgtg atgtcgagga agaccgtgtt cggacgcttc
    10441 ggcacttcca cggcaagaat cttgttggcc gcgcgtgctt tgacacgatg cggagcgtcg
    10501 ggcttttcgc cgaacgggaa gatggccatc gtgacgatgc gcgcttgcgg caccttcgtg
    10561 cgaattttat ccagcaccgc gagaacgcct tcggccgtct cctcggcgtt gttgccggga
    10621 tagttcttcg tcgcggagaa gttgttgccg ccgatgtgca ggacaacgag cttgggatcc
    10681 agtccgtcca tctcaccatg gtcgagacgc cacaggacgt tgcccgtacg gtcccagccg
    10741 tagccgaggt tcagcgtacg gtacgcgccg aacttctgct tccagcgcgg atgcgccgcc
    10801 tcgccgccga gagtgcgtcc acctgcccag aagtgggtga tggagtcgcc gacgaacacg
    10861 atctcgggct tgcgtgcgcg ggcacaggcc atcgcctcgt tgtgtcgctc gaaccagtca
    10921 taggaatcct tttcgatctt catgcgtggg cggagcgcga cgttgccgga gacgggacat
    10981 tcccagacgc gcacgtagtc gatgcggaac ttcgcaggca gatcctttgg atcgggcaca
    11041 ccgaaccaac tgacgaacac ctcgctgtcg agtgtgacat gcaacggacg gtggaagagt
    11101 ccgtcgttct tccgctcctt caccggcttg ccgtccagat accacacgag tttctccggc
    11161 gtccagagga gtccgtacac atggtagtcg tcgcagaaat cgaagtccac cttcgtcacg
    11221 aaggcacggt ccggcggatt aatcttcttc gcgttgatga caccttcaag gtatggcgta
    11281 cagtagagat ggaaggtgtt gtagtacttg cgcgagcagt ccggatttcg tccgcggctg
    11341 tcgggacgcc ccgtcacttc gaagatatcg atctcttcag aaacatcgcc cgccgtgtac
    11401 tttacccgcg gatcgtccga gtgggggtca tagagccaga acgcattgga gacgcacgcc
    11461 ttcattgttt tcgcgcggat ctcaaagtag ccgtagacct gctttttgcg gctcttgacg
    11521 atcgaggtgg cgtaggggcc atgccgcagg tgctcctgat gatgagactt ccgctcctca
    11581 tcggtgagga gccgcgcagt gaggacgagt tcgccgtcct ctacagagac gttgtcgggc
    11641 gcgaacctga agccgttaac gcaaggctcg ccgcgcatcc cggccgccac gcgtccggga
    11701 aacgacgcgc accaatcgtc ccacttggac ttgtccagcg ccttgccgtc aaactcatcg
    11761 ctcacggcgg gcacccgttt ccaccccttc gcctcctcgc aaaacggttc cgccgccaac
    11821 aagacgcccg ccgccaacag gcacggaaca aaaacggcac ccggtttaac gttcattgcc
    11881 atactcccaa tacgttgttt tgaatacctt gccagagatt aatgggatgc ccgcgtccgc
    11941 acgtttgact gcgtcggcgc gagcatcgcc gcatcgacca cgagccgttc cgtcagcaca
    12001 cacggcgaat cgtaaagcgg gatgtccgta taggtgatgc cgtccgaatg ccgcagcaca
    12061 cgttctttcg ggaattcata gatgcgccct gtaagcaagt caatccagac gggggtctcg
    12121 agcgcttcgg cgcctttcgg gacgtccatc acgaatggac gcagctcgaa cgactcggac
    12181 ggcggcacgc ttttcccgtc cttatggtcc cagaaaacgt aaagccgctt cccgtcgcgc
    12241 cggtattccc agatttcaat cgttccgtcc ttggaataat tgcggcgggc gacaagcttc
    12301 gtacgcgagt cgaaaagcgt cgcgacgttc tgcacggcat agaacgcgcg cttgacgccg
    12361 atgacctcat ggctcttgtt ggcccgcagc agtccgaatg tcccgagttc gcgccccaca
    12421 tgattgtagt cgcagatggt gaagacgctt gacgggatgc tccgtccaag gtcgccaagc
    12481 atacgccgca ggttgtattt ggcctgcgaa tactcgctcc acggccggcc aagcagcgcg
    12541 agcgtataca ccgtctcgct cggcgcgccg ttttcgccct gccagagcac ggcatggggc
    12601 gcatactttt tcaccagagc ctgacagtgc gcgacatcgc catacgacga ctcgggatta
    12661 tactcgtagc tgtggtagac gaatcgccag aagagtgccg tgtccggccc catcgccttg
    12721 agggcacggt cgaagcggtt cgtgtccgga tggttgaaga caagccccgc gacacgcgcg
    12781 tcgggaatgt tgcgcttcac gatcctcgcc gtccggacat tgaacgcggc gaccctttcg
    12841 ggcgtgtgct gcggattgtt gtccggctcg ttccacatcc cccaatcacg cacacggccc
    12901 ttgaaatgcc gcgtcagcgc gttgacccaa gcgtcccacg ccgcaagagc cttctcgccg
    12961 gacgggaatc ccccgccgag atccgcccgc acgtcgttgt aaatcggatt gccgtagctg
    13021 gtctcgatga gcgggttgag tccatgcgcc agaacccagt caacttgtgc gtcgagccag
    13081 gagaagtcgt agacgccctt cgtcctctcg cacttcgccc agccgccctg gaagcgtacg
    13141 ttgcggatgc cgagcggcac gaggaatccc ttgtactcgt cgaaattcgc gaagtcgcgg
    13201 tcaagcacct cgcatccgat ctgccagcgg tcgtccgacg ggtcgggttt ttcgcgcgcg
    13261 accagcgtgc cgatgcgcac aagcggcgga ttgagcggcg tctcgacgcg atccggcgag
    13321 cggtcgagcg tcacctcgac gcggccagaa tcgcctgtcg cggcaacgcc agacaacatc
    13381 gaaccgacga caattgccag caggccgttt ttcgaaatag tcatgacttt caagaatgtc
    13441 cttttgtcag aacctgatcg ccagacgccc gagatcgtca cgccgcgcgg cgaccttgcc
    13501 gtccaccgtg acggtcagtc cgccgtggcc gcggcgccag acgaccgaaa cctcgtgtcc
    13561 gcgataattg aggcgctcaa gcatgaaata gtcccatgcc gctggcgcga gcggattgac
    13621 cgtaaaaccc ttcgcacctt ctgggatgat gcctacgagc cccgaaatca cgaggtcgca
    13681 gaacgtcgag tggttgtagt ccttgccgcg ctcgaacttg actttctcct tcgaacgctt
    13741 gtgcaggatc gtgcgtgaaa tccagtccgg cttgtccgga ttcgcgttct catcgatcca
    13801 ggggacagcg atacccttga tgagcttctg ttgcgcggca tattgccaaa ggcagaagtt
    13861 gaacgtggag cgttgaggat tggggattga gggacgagcg tgcaggtcgt tgatgagggc
    13921 tgtgagcgcg attgatgtcg caaagggcca actcggaccg ttccactgac actcgtgtcc
    13981 gctgtaagcg aggacaaaac cttttgcgcg gcgctcgggg aacgtgaggc cgtacttcgc
    14041 cgcgaatccc tgcggatccg agagctgcga ccaatcggcc ttcttgtccg tcggcacgcc
    14101 gaaataccac ggcgcatagc cgtggagttc acggacggcg caacgcgtgc ggttggtgtc
    14161 ggccgcgacg aaaaacgcga tctcggggct ccagcagatc tcctcgagcc cgcggcggat
    14221 gaccgcggct ttttcggcgt agcgcgtggc gaggtccgag cggccgagac gcgcggcgac
    14281 ctcggaaatc gcatgcgcct cgctccacat cgccgcgttg aagagcggac ggtatccgct
    14341 cggcgtcagg gtgaattcgg tgccttcgtt cgtgcgggcc gaaaggaagc gtcccttgcc
    14401 gtcgccgccc atgcggatcg agcccttgcc ctcctcgtcc cacttccggc ggtcaaagcc
    14461 tttctcccag cgctcgaagt atgcgacagc cgcatccaga agttcgtccg ccaacgcctc
    14521 cttcccggcg actgccgcga atttgcagac gcccgtatag agccagctcg aatactgcca
    14581 gcggtgagtg cactccttgt ctgttaacca gaagcgcgca aggtccgtca tgtatttcgg
    14641 atcgcggagc cagcggccct cgaggaaatg gtggccagcg ggacagacga tcgtgttgtc
    14701 cttgccgctc cagccgacct tcggcaggaa ttccgacacc gtccagacgc ccgtgccgtg
    14761 acgcaggtgt ttgcggaagg tccaccagcg gaagtagtac gtgcgctcaa tgtccttatc
    14821 agggcaggcg aagagcggca cgttctcaga caagaagcgc tccgcgtcgc tgttcggaat
    14881 ggcattcgtc acgcattcgt tgtcaaactt gttgaatccg cgcacatagg tcgacaggag
    14941 cgccttcgtg tcaggcactt tcgaggatgg cgcctcaagg tcaagtccct cgtgcagcgc
    15001 catgccgacg tattcggacc agccgttgcg gccgttgtac cagagcatcc agcgattgtt
    15061 cttctcgtca cgcaggacgc tcggcttgta gcacgcgctc gcatcccagg tgcagagatc
    15121 cggggcgatg atcggatttt gcgcgatacg cttccagtcg ctgacgccgt caggcgagat
    15181 cgcgcaaccg atgcgcgcgg tgtcgatgtc ggaatagccg atgtagaaca tcgcccagcg
    15241 cccgtcggga agcggatgcg tctcgcaccc gccgacgcgg tcgcggtccc acgattccgc
    15301 agcgcccttg ccgaacatcg ggtttttctc ccactttttc caatcgaccc catccttcga
    15361 ttcggcgtag cagaggacgt tcggctcgta ggtctcgccg gcggcgtacc acatgcgcca
    15421 gaccccgcgt tgcgcgtccc agcggacgta ggggttcatc acagactttt tttcaaaatc
    15481 attctcggga accatcaccg gatccttgcg gacgcggaca aatttcatgc catccgcgct
    15541 tgtcgcatag ccgatcttcg aaccgccttt tgcctggccg gtataccaca tgtgccattt
    15601 gccgtcttta aagacggtgc agctacggtt caaatcgtcc tcccagccgc tcgtcgggtc
    15661 agcctcgagg cagatctccg gctcctgtgt ccacgacagc gcgtcatcgc tccgcacgag
    15721 cgcgatcgcc ttctttggtc gccacgagaa gtacatcgtg tacggggccg gaccgttcgt
    15781 aaccacgtta acgtcgaaac aggttccgag cttcgcattg cctaatactg gattcccggc
    15841 gtatttttcc caaaccccgc cgaatgcggc agaaaaggtg aaaaacaccg cacccattaa
    15901 aacgatcccg cttttcattc gaatgcccat cattttacct ccccgaagcc gtcgcaatcg
    15961 ttctccgcca gcaccttcta acgatctgac cgcagaaggg aagcgacggg gttcccttct
    16021 gttgaatcaa accgacgcca gtcgccagcc ggaacgacct cacctttgta agggaaatca
    16081 gccgttccgt gattgacgat caccttcgtc ccgttcgcgt aggtcgtgca agtcacattc
    16141 gacgcgagat actcgtgttc gtccatgaag acgaactgca gatcggaaag acggcagaac
    16201 tcgtcatatc cttgcttgat cttggcgact gacgccgtca gttccgcgtc ggtcgcacac
    16261 gtgaagtcct catcacccat ccagcggaag ccttcgacga agttcgtata gaaatagaaa
    16321 atcggacgtc cgccgaactc gacaagcttg aggcgcttta cgttgtcctt gatcgtatag
    16381 ttgaccgtgc cgcagaaagg attgatgaga acgatgccgt tgtagatgat ctgggcgagc
    16441 ggcacgtgcc ggtctaccat cttgttcagt tcgcccggta cgagcttgta gaagtaggcg
    16501 tataggcaat agtcgaggtc gccgacgcag tagtcgaaga tgccctctga cgcaaagccc
    16561 ccgagacgct tcttcgtttc ggactggatg cgcgcgtacc attttatgca gtcggcctcg
    16621 ttgatcgcat gcctcgggtc gtaacatttc ctgagcggct gggacgcgat gacgtcggta
    16681 tagtggagtc cgaagaaccc gagatcacgc atcatctcca gatccttcac gaggaagcgc
    16741 tcacacatcc gttgcggaca cgtcaggtac tggaagccgc cgccccacgt tccagccgtt
    16801 ttgggcaatc cgtccttctt ttttagcgtg aactcctcgt cgaagcagtc cgcgatcagg
    16861 tatgcgtccg aatagttgtt gtgcgcgacg gtctggaagc ccattgtctt cgcatccttc
    16921 aacagcgcgc ggagcttcgt ctcgccgccc atcttcggtt cgacagggaa gagctgcgga
    16981 taggccccgt cgtgtccacc cttgttccaa ccgacaaggc aaaactcggc ctcgggaatg
    17041 ccctgccgct tgaattcccg cataatgtcg cggcaacggt cgaatgtaat cgccaccttg
    17101 acgggcggtt cggtgaacgg cgtctggtca cgcactttcg cgggcacggg tttccagccc
    17161 agccgcagcc gcacctcgat gttcgtcgcg gcgtacgcga gcgcggggaa gtctttcatg
    17221 cgctcgttaa tcggtctgca tgcgccacgc ttcagttgat catcgcggta ttcgcgcgcg
    17281 aacgcgcacc aagacacatc cgccgcgccg aaacggaagc ggatgacaag atcctcatag
    17341 gggggatcgc cgttgaagtt gtagcggttg aagagacggt atccgccgtt cttcgcgaca
    17401 acatgcgtct cgtacgaata ggccagaccc gtcgcatgcg ccatgaaatt cacgcgcggt
    17461 gtcttcatga tgaagagcgg atggtagttg cgcgcggcag gatagaattt tccgttgtcc
    17521 gcacggaacg taccgtaaag gccgtccggc atgaggaagt agccgtcttc gccctttttc
    17581 gccgtcgcga aatcgggcgc gacatcaagt gtcttcacgc ccggtccgat atggtctgcc
    17641 ttgacgacga gccggttctc gctatcgagc ggcattttct cgcgacgggt acgtccgtcg
    17701 atatacgtgt agtcaagcgt gacttcagcc gcctgtacgc agggtgcggc cgccgtcaga
    17761 accgataccg cgaaaacggc aagccgaatc gtcttgtgac cggtgccgaa ggtgtcagcg
    17821 ttgcaaatcc ggtgtgctgc atttgtcaga tcgttcttgt tcatggcttt gagattgttt
    17881 cagtggttga gtatccatgc ggccatcgct ctgccgcaat acacagacag gagtgcgatg
    17941 cgaacgcctt accatttgtt ttcagtggca aatggcgaag ccggcaacgc ttcacggttg
    18001 tagagattgg caagacccgt cgcgttggcg cgatagccgt agcggacgtt ccggatcgtc
    18061 ttgccgccgg ccgtcacgac gaccttgtca cccgcgatct tcgcatcggc ccagacgaac
    18121 tgtccgccct cgacctcggc cgcgaagccg acgagcttcg cgtcggggag gagcttgacg
    18181 ggcgcgttgg agagattgtc cttctcacct gccgtgagac cgccgccgac gtagtcgaac
    18241 gacagcgtca cctcggccgt gccgagcccc tccgcttccc tgaaaagcgg acccgagggg
    18301 acgacgtccc ggccatacgc gcctttgagt gcccacgccg cgagacgctc gcccacgaac
    18361 accttggcct tcggatggat gtccttgtcg ttgccgacgt caatcgtgac cgccatgccc
    18421 gtcttttcca gacgcttgag cgcaaggcgt tgcgcgtcac ggatgagcgc atagccgttg
    18481 ccgcccttcg gatcgtctgt cttcgccgtg aaagacgcga gctgcaccca gtagaacggg
    18541 aactcataac cccacgcctc gcgccagccc ttgacgagca tctcggtctt ctcggtgtag
    18601 gactcgccct cctgcccgtt cgagcaaccc tgataccaga tcgcgccggc gatcggaaaa
    18661 cgcacgatcg gcgcaatcat cgcgttgtac tgtgaggtcg cgctgacact cggcagcgcc
    18721 ggcatgtcgg cctcggggag cttcccctcc gcgacggccg cttcgaccgc cgcgagaaac
    18781 gccttcatct ctgcggcgta cttcgcgaac accttccggc ccgcctccgt gcgcggatcg
    18841 gtcttctcct gaagctccag aaagctctta acctgcttgg cgtacgcgcc gttcgttgcg
    18901 ctgtagttct tgaacgacga gagcgggatg aacggctcga tccggcaacc gctccagttg
    18961 tcgtcaagga tgccgatggg gatgtccaac tcttggacaa gcctgcgcgc gaaaaaatag
    19021 ccggtcgccg tccagttggc gatgccgccg ttcgcggtgg tacgggccca aggatgatcg
    19081 agcttcacat cggctttggg ccacgttgag tggatcttcg agaccttcac gcagcggatc
    19141 gggtcgaact tctcggccgc ctccttcttg aaggcctcgc cgtcgtagat gccccagttg
    19201 aagctcatct ccatgttcga ctgccccgag cagagccaca catcgccgac gaggatgtcc
    19261 ttgaactcga tcttgttctt gccctcgatc accatcgtcc ggttctcctt cgaggccggc
    19321 atcggctgga gtttcacctc ccacctgccg tcggcatcgg ccttcgccgc aacggactgc
    19381 cccgcgaacg agaccttgac ctcttcgcca gcatcggccg tgccccacac cgggacgacc
    19441 ttatcgcgct ggagcaccat gttgctcgag aacacattcg ccggtttcac gttcgccatc
    19501 gccgcgaacg ctgccatcga acaaccaata atccatgtct tcttcatcct gaatctcctc
    19561 gtttccttat tcctgtttcc caattcccat tctcacttct tctccctctc ccaatcgtcc
    19621 gtgcagaacg gcgaagcggg aagaagctcc cggttgtaga ggttcgcgcc gatcggcgac
    19681 gtgcgataag cgtagcgcac cgcgaccggc ttcttcacgt ccttcgacga aacgacaacc
    19741 gtgtcgccgt cgatgaccgc gtcggcaaag aaccatgtgc ggtcctcgcc tgcgatcgca
    19801 aatccgctca acttcgcctc gggcatgagt ttcacgtccc tgtcgtcgaa gttgttcttg
    19861 tagccgacgg cgaggcccga cccgacgtag tcgaatgaga gacggattct gtcaccctca
    19921 atcttcatcg cgcggtaatt cggccccgac ggcacgatgt cgtcaaagcc gtactcgttc
    19981 ttaagcgccc actgcgcgag acgctggccg acgtgcagct tttcccgcgg atggatgtcc
    20041 ttggggtcgc caaggtcgaa ggtcacgcac atgccgccgt gtggaatggt cgccatcgcg
    20101 cggcgcatgc cctcgcggac gcgcgtgaac ccgagtccgc ccgccggcgc gtcgtcgacg
    20161 acacccttgt tgtcgaagga cgagagctgc acccagtaga acgggaacgt gtacccccac
    20221 gcctcgcgcc agccgcgcac gaggccctcc aggagatact ggtagttctc gttatccgga
    20281 tagttgccgc acccttggta ccagagcgcg cccttgaccg gatacttcgc gaacggatgg
    20341 atcatcgcct ggtagcagcc gttccagtag gtgtgcgaga cggggacttc ggcgggatcg
    20401 ccggccggcg gccggccgtc gacgaccgcc gcctcgatgt ccgcgacgaa cgccttctgc
    20461 cgggcgaggt acgcgtccca tttcatccgg cccttggcgg tcgccgggtt cgcgttttcg
    20521 tcgtagcggt ccttgatcgc cttcagcgcc ttcgggtcgt cgtcccggag gcacgtcggg
    20581 tcaatgtacg gttcgatgtt catgccgccc cagctcgagt caatgagccc gatcggtatc
    20641 tttttcccgg ttttctccat gacggtctgc gcgaagaagc agccgacgca cgaggccgtg
    20701 aaccacacct gccccgtcag cacactccac ttgccctgta tctccggcgt ttcgcgcggc
    20761 acgggcgctt tgtggcgcgc cacgcgcaaa tagcggaagt tcggcgtgtt cgtgcagaag
    20821 gccttcgaga ggtccatgga gtacggccac cagcagaacg agtattccat gttggactgg
    20881 cccgagcaga gccagacatc gcccacgagc acgttcgcga acgtgacgcg ctcgccttcg
    20941 ggcgcacagc cgaagagcct tgcgaagaag ccttgttttt cgcccatcgt cacggtcatc
    21001 gtccggtcat cgttcgacac gccgagcgcc ggcagcgtcg ccgcccagcg cccgtccgcg
    21061 tccacggttg cccgtgcccg cgcgcccgcg aagtcgaccg tcacctcttt ccctgcctcg
    21121 ccccttcccc agacggggat cggcttgtcg cgctggagga tcatgttgtc catgaacacg
    21181 ggactgaccg agagccccgc cagcagaacg gccggcgcgc acaccgccgc gacgatcgtc
    21241 aatgtcttca tcgtctcctc agatcattct tgttcatggc ttagagtttg cgaatccgct
    21301 tcccgtgcag gccggggatg tgggggttcg ccgcgatggg caacgatcat ggtcacggac
    21361 gcgtcccggg cttgaggtct tgatacaagg gcaccttgtc gccgatccag tgtccgccgc
    21421 aggatttgat ttcgcgttcg ccgttcagct tgccgatctc aacgttctcg agcgtcatct
    21481 ggcacgtgcc gctgttgcgg atgaggtgtt ccgcctccgg cacccgggca ttgcggacgg
    21541 tgatcgcgcc ctccgcctga tggaagttga ggaagctgaa gaggcggacc gtctcgtccg
    21601 cagtgatccc ggatacggtc acgttgtcga actcggcgtc acactgcacg gccagcgcgc
    21661 ccttcgcgcc gggaaggagg tgcaggttct cgatggtgac gttctgcatg ggacagaaca
    21721 ggcggattgg gaacccggcg ccggaggtga cgttacggat gacgatgtcg cgcatctcgc
    21781 ccatcgcgca gttgcgtttg cggtagtatt cttcagcgct gtcgttgatc tgcacggcgc
    21841 ttgcgttgcg ttgccagttc atccaactcg gatcgtactc gtcatgcacc ccgtcgatga
    21901 cgacgtcatg cactttcgca ccgtcgcaac agagaagccg cacctgatta aaccagagcc
    21961 aagtccccgc acggatgttg cggacggtga tgtgatggat gtcggtcgac ttgccttcga
    22021 cgatgtcttt catctcctcg ccgcagaggt tggtgagcgc gaccacgtcg tcctctgtcc
    22081 agccggtgat gttctcgaca acgatgtcgt ggcagccctt gcgcaaattg acgccgtcac
    22141 cgtttttcac ccaaaggttc acgtatttac cgggcaagcg gttgggtcga tgatccctgc
    22201 cgtcctcgct cacccaagag atgtccgcct cgaagcgcag atcgcggatg acgccctgtt
    22261 cacagaagct gaagcaggtt gcccagtagc gctgatggcg gcatttgagc cccgagacct
    22321 tgaacccttt aacgttatgg aagtagacga agcagttgtg gacaagccgc tttccggcga
    22381 gttcgggcgg cttcgatcgc tcgcccagac cgttccagtt gccgccgtcc agcgtggctc
    22441 cgttgcggcc ctcgatcgcg atatcgcgat cctcggcctt cgccgtacac cggtcgtccg
    22501 tccaggcgtg ctcgttcgcg aaaagattgc aacaaacccc gtccgcctgc acgaggcgcg
    22561 cgccgtccag gatcactttc atgccagacg ggatcagcac ggtctcctcg atgatccact
    22621 tgtcgtcgtt ggccgcgtca ttgcgatatg gaatcaccac ctcgcgccgt cccgccgctt
    22681 gggcttctgt gatcattttt gcgatccgcg gcgcatcccc gcgcgcggtg tacaacggcc
    22741 cgccatccga accgggtctg gcataggcct tggtcccgtc cgcgaacgtc accgttacag
    22801 cgagccccaa catgaccgca atgcacattt tcatgatatt ccgcctctct tacctcttcg
    22861 tttctccggt cggcaagtgg tacgcgcctg tcaccttacc gaagcgcttc gtgaacacat
    22921 aatgggggaa caggtccttg ctcgtatgtc ccgcatcgtg accgcgattc tggatgatcg
    22981 tgatctcggc gaacgcggtt gtgtccggga tgccgttgaa catcgccatc atgccgtctg
    23041 cgggcccggc agaatcgcag atgccgaatg tgtatgtgat gttctgggct tttgtgcgtg
    23101 tgatgtggtg cacgggatcg gcgaaatccg ggcccggctg ccataggggg cgccagtgct
    23161 tttgcgatcc gccgacgtcg acgtaccacg ggcacatcga ccacgccttc gtgatctgcg
    23221 ggaaaaggcc cgagagcgcg aacgtctgga atccgccctg gctgtcgccg tgcacataga
    23281 ggttcgtgcc gtcccaaccc ggatggctca tcgcccagcg gagcgcacga atgtcgcgcc
    23341 gcaacatgtt aatgtgataa cacgtctcga ccttcgcatt ctcgttcgtc gggaagccga
    23401 agttgccgtg gcgcttacgc gcctcgtccc aataggcgtt cgacgcgcgg ttcgagatcg
    23461 aatggcagtt gatgccgagc gtcagctcgc cgctgatcca cgtctcggcg aagtggaaac
    23521 cggttccata cgcctgcaga aagacgcgga tcggcagctt tcccttgcct tcgcgcacca
    23581 ccttcggcac gccccagtca aagtgcacct cgccgccggg cgagtccagc actcccgcgt
    23641 atacccacac gttctccgca tcgtttgtgc cgcggcgccg gaagtgtttt ggcacgttgg
    23701 agtatttcgc gtcttcttcg agaaactccc gccacttcgc atcgaaaccc tcggggatgg
    23761 gtttgccggg cttgatctcc agcgggttga agagcgcgcc cacgcgatag ctgagcgtga
    23821 gcgaatggcg cttgcccttc tcatccacgc gcgcctgcct gagatcacgc acgttcggat
    23881 agcgtccgtt aagcggttcg ggaatgccgc ggagcttcgc tgtcacggcg acaacgccgg
    23941 gcttgtccag cgtgaggatg cgccgcacgg ggtttccctc ctgcgaaaga acgatatcct
    24001 cgaccgtcgt ctcgtgtgtc cggtaccacg agaccgacat cgtcacgttc gtgagctgct
    24061 ctttcgtgag aagtcggccg tccgccccgc gtacctcgaa aaccgcctcg ggccgctcgc
    24121 cgacctccca gacgatccgg tcgttcggca cataggccga taacgtccag ggtcgttcat
    24181 aatccgaaag cccctcggtc acgagcttcg ccttcgcgaa ctccgccgcc atctgcgcct
    24241 gcacggccgg cggcagcggc gctttctcct gcgcccacgc cgcaccggcc agacagatcc
    24301 ccgtcatcaa catcatttgt cgtttcatgt ccatacccct cgtacactgt gttgctaaaa
    24361 aaactcggac atgactacat gatttacatg atgtgcccgt tttattccag tcataatgaa
    24421 tcctgtaatc ctgtctgaaa gcgttgtttt gggttttatt gattgctggc tgtatattcc
    24481 cgtcaatgca tgtcactcgt ccttgaggcc gtccgcctcg ggagagccgc cggcgagata
    24541 accgcgggac cggcctgagg cgtcggagaa ccagaatgcg taaagttgcg tatcgcgggc
    24601 ggtgaagcga agacgaatgg ttttgcccgc gtaagaggaa agtccgccga gaatctccga
    24661 tttgacggca ttcgccgtga atggcttgga agccgcaagt gcttggccat cctcatccag
    24721 aatctcgacc ttaagctcgc cgcccgcggc attcgcgttc acgaagaggc ggtcgccccg
    24781 ggtgaagcgg atcggacgcg tgacgagttc gcctccgcaa cggccggcat ccattgaggc
    24841 gaagccgtcc cggcgcaggc gcgcgatacc catctgcgcg agcgtgtaaa gccctccgac
    24901 cggatggtct ttccactccc cgatctgtcc cgcaaagccc gtgtaataga agagcagctc
    24961 gtcgcccttc acaaggcaga tcgccgcgtt cgaatgcaga taagcgcggt cccacgcacc
    25021 gtatgtgcgg gacgcgggta tgaacggcga acggtcttcg ggacgcgaga agtggtagcc
    25081 gtcgcggctg aagccgaggt ggagttcggt gagcttggga gagccccgac gctggaactc
    25141 gctgttgtcc gctccggaca tgatcgcgta cgcgcccaac atcaacgatt cgtaggcgac
    25201 acaatcgagg ttatagaggg acggctcggg cggttccttc tcgtagttgc cttcctcgca
    25261 gtagacccag cgcatgtcac gccgatcaca gcagtccgcg cgttgccagc agacgcggtt
    25321 cgtgtaggcg acacccgcga caaagtcctt gctctcggca agctggcgcg agcgggcaga
    25381 ccagaagtcg cgcagcgagt agcaccatac cttacggaac ggattataga agagggtcga
    25441 gcggtcaccg ctccaggcac aggcggcgcg ccactcccag tccgtgccgt tcgcggacgt
    25501 gaaaatctcg ccgccttggg gacgtgacca aatgaacgcc ttgaagcggt tgccgtccgt
    25561 cgcgtccggg tcgaggatga ctgcggcgga gtcgcgatga cagttcttgc cgtcccgcgt
    25621 ctcgggcttc acgatacggt ttgtcttgcc gtccaccttg agcaccgggc gctcccagtt
    25681 gtagccgtcc ttggaatacg cgtacgccgt tccgtcgaac cagcccgcgc agtaccagca
    25741 cttgtagagc ctgtccgtcg cgtcgaacca cacgccgccg gaaaagggcg ctgccatcgg
    25801 cgttttgatc ggcccgagcg caccgcgctc gagcggggtc tcgggacgga gcaccgggtt
    25861 cttcggatcc ttctgcgcct tatgccaagt gcgcaccatt gtggtcgagg cgatgaggaa
    25921 gtcgtccacg aaaagttgac gcccggcatc cacggggatt actgccgggg gattgtccag
    25981 atacggcacg ggcagcggat agcgtccgta cttgcgcata tcagtccggt cacgcggatc
    26041 cgtctcgagg acgatcccgt tatacagcgt ctcgcgcggg acgtccttct tcacggttac
    26101 ggccggcttg ggatcgaacc ccgcgatgac gcaccagtag tcgagttcgt agcgttcgcc
    26161 ctctttcacc tgtttgcgcc agacgcggac cttctccttg tcggacgtgt tgaagagctg
    26221 cgagacggga atgtcattca cctccgcgaa cccgagcttc ttcagttccg attcttgcga
    26281 aggcttgccg cccttgtcgg tgaactccgc aggcggcgtc agcgccagaa tttccccgga
    26341 ctgcgtcacc cggaaggaga tgccgttgat ggatgcgtag acaaaggact tgcccacgag
    26401 ctgcggcgca acctttttgg aaatgacgaa atgccggtcg aagaacagcc gctcgccggg
    26461 tgcgaattga cggatctcgg aacctgcgca cggcttgatt ttcgccgtcg gcgcaccgtc
    26521 tgcggcaaac ccggacaagg cgcagacagc ggccgccaga acggacagtg tcaggacttt
    26581 ttcaacattc atgttcttct aaggataccg tttgatttta aggacacccc cgcgcgtgcg
    26641 ccctccctga acgcatgcgc ggggtttttg tcactcacgg ggggtcattt gacgctcatc
    26701 gtaaagacaa gcgggcggaa aagctcgaaa cagccgatgt cgaccctacc gccttcaaca
    26761 cgcgcgcacg gatcgatctt ttcgagtttc acgcccacag ggaaagtctc gccgaacgcc
    26821 gtgatcgtgg ctggatgggt ctcccggaaa ccgttgccca tgtccagcgc cccttccgtc
    26881 atccacgcaa ggttggtgcc tgcgttgtaa cacgccgaac gccgcgtaag cctgtaatcg
    26941 ccctgcgcac tgtccctgaa catcggatcc gacccgatca cattcccgtc tttgtcgaaa
    27001 ctcgcggcat tcgaggcgcg cgaatcgata aagcagtgcc gcaccacgtt cgttcccgcg
    27061 taccagtcct ccagcgcgcg gctcgtcgtc gtgtcctcaa ggcagttgtt gtagataagg
    27121 ttgttgcgct ggaaaagatc atattggctg cccttaagcg catacgacga acccaaaagc
    27181 gtgttgtcgg cgatcgtgca gttctcgact ctcccgcaag cgccctcccc cgctgcaatc
    27241 ccggattggg cgacattcca ctccaaaata tacagcacat gtttcaccgt attgctgacg
    27301 aaaagacagc tccgtaccgt cgcgttcgac gtgccgaacg cgtaaaacgc cgcgccatcg
    27361 gatgtgctgc tgttgttgat gaacacgcag tccgacaaaa acgtcgatag tctctggttg
    27421 ccataataaa cgacaccgcc accgtatttc ccggcatagg tgcacaggtt gctgtcgaac
    27481 aggcagttcg agaccacggt tccgccgtgg ctctgcatcg ctgagcccaa tatccccgag
    27541 ttgttccgga acgcgcaacc gtgcgcgaca ccgttttggt cgaggtataa accgccgctg
    27601 ccgcactcgt tgccgccgcc gggcaggtca ttgccctcga agtaacagtc gaaagccgct
    27661 attccatgca ttgcggcgcc cgcctgcgcg ctgccgatgt tgccgatgaa ggagttgttg
    27721 ccgcaggcgt ataccctgtc gaggaaagcg cccgagccct tgccggtgat gtagttgcct
    27781 tcgaaatagc aattcgagac cgtggtcagg gccgtaaaca acccgccgcc gcggcagttg
    27841 taaatctcga gcgggttgac gagtcggttg ctgataaagg cgcagtcgta ccaactgccg
    27901 ccatgcgcgc cgccgccttg cataaagtta tatctggcca caccacgggt gcagactgcg
    27961 gaacagttgc ggatgatcga acggcgtccc gtggagagac cttgcacacc gccgccattg
    28021 catgatgtcg cattcgtgta ggcttctgtc acggtgttgg agatgcagca gtgctcgacg
    28081 agcgaatcaa tgagatcccc cccgttcgct ttgatgccgc cgccggcaat tccaatctcg
    28141 cctgtcccga taacggcgca actggtgacg atgcagtttg atatgaccgc gccgcccttc
    28201 gtaaggcaca ggccgccgcc gccataggcg ctcgtcacct cgcaacggcc gttcgtgaag
    28261 gtcagattat ggaagtgcga gttgaaatat gtgctcgtca cgcagttcgt gagttgctgt
    28321 gcgtcaatga ccgtattcgc ggcgaccccg tcgtcaccga caagcgaaac accgtctgcc
    28381 ttgaatgtca agggggctga aatgagatac gaccccttcg cgacatggac gatatcattc
    28441 gccgaggctg cgtcgagtcc ttcttggatt gtcagaaaag gcgtcgcctg gccgccgtcg
    28501 tgcgtgtcgg ccgtcgagtc tttgtcgaca taccactctg ccgcctgaac tgccgctccc
    28561 aagattgtag cggcaacgat gaccgtggcc ctgaacgcct ttgttttcat catcactcct
    28621 cttatttcac ttgacataca tgcccgtgcc taagggcgcc caggattcga aacagccgat
    28681 atccggcacg gtcgggaaga tgcggggcaa cgcaggaacg cgcaccacat cgcatccgcc
    28741 ttgcgtcagc gtttcgtccc acaccaccgc ctgattggaa agatcgttcg ccccggccat
    28801 ccacggctgc gctttacccg cgtttttaag cggagagcgg cccttcaaac ggaaatcgcc
    28861 cttggcggga tcggcaaacc cggggtcgat ctcggtgatg ttgccgctca acacgttcgt
    28921 cgtgaacgcg tcgaccgcga tcgcctgcgg cccgatatac gagtgcgaag tctgcgccgc
    28981 catgctcgca ttcaggttat agttgtcagc cgtcccggcc ttataatttc cccacaccgc
    29041 cgtgttgcac acgtaaaact tcttgcccgt ttgggcaaga atggcttcac tcgtgccgct
    29101 caggcggttg cccgcgatcg tgcagctctc cacccgcgtt ccggggaact cctgcccgat
    29161 gtccgcgacg ggattggaga tgtccagaac ccgtgaagag gcgttaccgt aaaaaaagca
    29221 gttgcgcacc acggagttgg tcgttgaccc gacctcgttt tcgcgtccgc tctgcttgac
    29281 aagcggttga ttcccgttat ttccgctttg gttgccgatg aacgaacaat ccacaaggga
    29341 gaagttcagg ccggagttaa tcgccaagag ggcgtgtgtg ccgctgttgg tggcgctcag
    29401 gttggcctca aaatagcagt tcgaaaggga aacgtacgcg ccgccgtttc cagaccaagt
    29461 ggagagagcc gccatgccaa agtctttgtt cacgtttccg acaaacgcgc agtcatggat
    29521 aaagaggccc cgataccccc cctgcgtatc attcaggtgc gcgtgaaccg ctgtgccgga
    29581 accgtcagca accccgcctg gcgagtaatt gtccttaaaa aggcaatgcc ggataaccgc
    29641 ttcatttcct gccccaagtt ttaggcttgt cgtcaaagaa gcgccgtagc gctccgtccg
    29701 gttgccgaca aaggtgcaat gcgtaaacag cgcgggagcg ctggcattga cacagaccgg
    29761 ttgggagacc gacgaattcc cgacgaaaac cagattggag aaaactttgc cgctcccctc
    29821 ggtaaagacg gcgccgcccc ttcccgccgc tttgtcggtt ctgctgctcc aaacgcggtt
    29881 ggtctcaaac cggcaatccg ccgtcagtgt cccgacgcca tgcattgcgc cgccgtccag
    29941 gtccccccat ttccccgtcg gatcgacaat gctgttgcgg cggaagatgt tgccgcgaaa
    30001 ctccttgtag ccgccgccgc atatcgctcc gccgcggaaa cggaggcctg tgagggtttg
    30061 cgactctgcg gacgcacagg cgttggtgat gccgttgtcc tcgaagaggg tgtcgacgac
    30121 cgacccgttc gccacagcga ccgcaccgcc gtaaatgcac cccgccgtac cgccttcgcg
    30181 gaaaccgccc gaccagcagt tccggataat gcaattcgag ataaccgcat cagagttgcg
    30241 tgcgttgatg gcggaaccgc catagagctc caggccggtt tcggcaaaga catctttccg
    30301 gcaattgacg atggtgagcc cgtgaaactg cgagcctttg gcatacccct gaatcgcctc
    30361 aaaagcattg gtggcgtttt gcccgtcgat gatcacctgc tcggcaaacc cggggttcgc
    30421 cttcagcagg acattggtga caccgtagaa cgtgaccggg tttgaaccgg gaatagcagg
    30481 aatcggctcg gacaaagcat agtgtccggg cgcgatgtat atggcgctcc cgctctcata
    30541 attattcgta agcaaattgc tgacagcctc gccgatggtc ggatacgcgg tttcccacgt
    30601 catgccgtcg ggcatgaccg cagaagtgtt cagatccacg tacaccgtcc tggcccgcac
    30661 cgcgagggcg cacacactca aaacaacaac cagccctaca tttctcatga tgaatctccc
    30721 cccatcacca cctattatgt gggggagggg cgaaaagagt caatcctgaa agcccgcgcc
    30781 aggcgaaaaa gggggagggg gaacgaatga aactcgtttg acaggcgact gtactctttc
    30841 gcataatgcg agtggaatcg attgacggag ggacatatga aagacggtgt cagattggtg
    30901 tgtgcgctgg caatgccggc gtggatcctg cacgcggagc agccgtgcga gaattggctg
    30961 tcgccctaca cgggcgcgga tgcgaccggc gagaaggtgg tggggctgtg gactttcgca
    31021 gacttgaagg acgcgtcggg caagggacgc gacgcggtgc tgcacggcac cgtgctggtg
    31081 ccggatgccc gttggggttc ctgtctagag tcgttctgcg gctggccggt ggaagacaaa
    31141 cgccacgcgg ccgttgtcgc caatcacacc tcgctctcgc cccggggcgc gttcacgctg
    31201 gagatgtgga tcaagccgaa gcctgagttt gtcgattatc ccgaagcgtt cctgatcgac
    31261 aagaagtacg tggcgcacac ggattaccaa tggacgctgg gagccgccga taaaagcggt
    31321 cagcggcgcg cctatctgcg gctcggtttc ggcgacgatt cggaagtgta tagttcggag
    31381 ccctcggtgt ttgcgacgaa catctggacg catctcgcgg tcacctacga cggcgctggc
    31441 accgtgcggt ttttcaggga cggcaccgcg caggggacgg tcacccggac ggggcgcggg
    31501 cgtatcgcgc cgggcggcca tccgctctcc atcggcgacc gcatcggcag ctactatcat
    31561 ggctttccgg gatacctgac gcaggtacgt ctatgtcaag gcgcgttgga gtttcgtccg
    31621 gtcacattgg ccctgacatc cgaccgcagc gtgtttgtgc gtatggaaaa gacgccagcc
    31681 ttgcgtttca aactggccaa tgcccagcgt gaggccgcgc acaacatctc tgtgctctgc
    31741 acggtcgagg gacgcgaggc cgcgcgccat cagataccgg aacttgcgtc gggtgagagc
    31801 cggacgatcg aggtgccggt cgatacggcg ctgcgtcccg gcagttatac actacga
//
LOCUS       DAOSRB010000007        31316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1600_length_31316_cov_7.066761, whole genome shotgun sequence.
ACCESSION   DAOSRB010000007 DAOSRB010000000
VERSION     DAOSRB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 31316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31316
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1600_length_31316_cov_7.066761"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1710
                     /locus_tag="PLZ74_00825"
     CDS             127..1710
                     /locus_tag="PLZ74_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96898.1"
                     /translation="MKAFLNGSVVLLCFAFALPRRGDAEPVLLFSDTFDYVTSGSVSN
                     PSSSTWKSSELYSMYTNIYSAGGSVKLGKSNEGGILTTTNLAVTAGLLSVQVDALGWD
                     SDEKGFMVSVGNMTTNLTCTNDKSASTLFETVSTTFEVETGVIFVTFQTENGKRIFLD
                     NIQITHTPEGGPVPLAFFVYPHSATTVPALTEIAFTITALADGVATNVSYAGGLPDGA
                     LYDFIGQRFAWTPELNHTGEFALVFAATDTAGTVHQKTVPVTVTPLPLHAPTGVTVTE
                     LTCKTVSLAWDAVPAASHGYRVSAWYGSTAPDMLSVDGETFFEIMDTGLVAAPTGWLF
                     QGVTEKYSTADFVELKFNDTGDSVITKRYPAPVSSLSFRLKGYATSSLSNSLFTVYGS
                     CGGDDWTLLRQYSSLEDSDGDDENNLFTSAEGDLDKTVTLHAAEGYRQFRFVYGQKAS
                     GNIGLANVTAAYAGSGARFVDGWQATPVSATNLRITGLPPAHEILIRIEALDATESRA
                     TQLRLSSPPFLPKTLLMIR"
     gene            1982..3310
                     /locus_tag="PLZ74_00830"
     CDS             1982..3310
                     /locus_tag="PLZ74_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR96899.1"
                     /translation="MNRRDFLKTTVYAGAVAMGARTASAQASPAERVRVAVMGTGGRG
                     ASLMKTAAELPNVEIATVCDVDVRAMDRAAETVSKKTGKTPKKEKDLRRILEDKEIDG
                     VICATPDHWHAPAALMTMRAGKAIYVEKPCSHNPREGEMLVEASVRHNAVFQMGNQRR
                     ASMTYRQVIREIQGGLIGEPRFARCWYTTQRGPIGKGKAVPVPEWLDWDLWQGPAPRR
                     PFRDNIVPYTWHWFFHWGTGESGNNATHFVDVARWTLGGRFPTRVTSAGGRLFYEGDD
                     WEWYDTQTATFEYPNRTFITWEGLSAVKARPYEGIGTGCMVYGLKGAVLFAPNNTCRH
                     FDPAGKVVKNWDAQSLAGDAMDRANPIGGLDAAHLGNWADCIRARDRAKAYSPADEAH
                     ASTLMTHLANIAQRTGETIRTDPATGRLAQGSPGAELWAREYEKGWEASV"
     gene            3328..4224
                     /locus_tag="PLZ74_00835"
     CDS             3328..4224
                     /locus_tag="PLZ74_00835"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOR96900.1"
                     /translation="MSPVMRHLSRRMFSAQALLAAGGWAAGVWRASADAKRPLKVYIF
                     ADMEGISGITHSGQTLADGAAYGEGCRLMEADINACVAGCFDAGATEVTVRDGHGRGN
                     NVDPARVDPRARLVQGPTPGVRFGGFEGSAALILLGYHAKALTPEALLAHSYSSLTIQ
                     GMWLNGREAGEIGVDTAIAAEHGVPVVMVSGCDKAVAEAREWIPGVVTVQTKRSTGVQ
                     SAVCLPLESARRMIREKAAEAVRRRQAIAPVAVAYPVTLRMDFLPQGSLRTYDPAFTP
                     TSSPRRVERTGDSVEKLLLNKN"
     gene            4235..5233
                     /locus_tag="PLZ74_00840"
     CDS             4235..5233
                     /locus_tag="PLZ74_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR96901.1"
                     /translation="MRKTVRIAAAFVLACGAVYAEVTQVGIIGLDTSHTIAFTKMLNV
                     EKGEPLFESFRVTAAYTYGSLDIPSSTNRYPAYTKQMVEMGVEIVGSIAELLKRSDVI
                     LLETNDGRRHYEQALEVFKAGKRVFIDKPIAASLTDAVRIFDAAKHYGVPVFSSSALR
                     YSTKIQKAHAGAFGRISGADAYTPCALEPTHPDLFWYGIHAAEPLFTVMGTGCESVVR
                     VSREKVDFVVGVWSDGRVGTLRGIRDKPAPYGCEIFAEKGGRLALGGYEGYKPLLIEI
                     LKFFQTGRVPVSPDETLEIFAFLEAAHESTRRGGVPVKVAEVLDQARAEAARKGMK"
     gene            5236..6909
                     /locus_tag="PLZ74_00845"
     CDS             5236..6909
                     /locus_tag="PLZ74_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96902.1"
                     /translation="MQQNGLAFADYAVVVSFFAVMISVGVFFGRSQKNMAQFFGGGKQ
                     VPWWLSGISFYMCSFSALAFVMYSALAYKFGWLPITISWLSVPAVFIGCRLFAARWRR
                     AAETSPLEFIERRYGNSMRQGLMWLGLPMRVLDDAFKLLAIGTVVGAGMGFPLHGAII
                     ASGLIILSYTFMGGLWATLVADFIQFFVLFTVILVLPFLCLDKAGGWSGFVENVPPGF
                     FRLTADKYNWTYMLVFFLMLFFNLSTSWSMVQRYYSTRSEKDARKVGYFVCILLSLGP
                     PLFFIPAMAARVFMPEIPADRMNEVYALVCKNVLPAGFMGMVIAAMFSATMSTLAGDY
                     NAVASVVTNDFYKRMIAPNASPKQHMLVARLATLLVGLAVIGLTFVMQNAQGANDLFD
                     TANKVFGIFLPPIAMPTMLGLVTRRITRRGGMAGLWGGVIVGLVTFIAGARWPVLRVT
                     SVIFFITCFATLAGLFLGSLLLKDDPAHMESLNSFFEKVNTPSPVLEGPPARITFWPL
                     VAGGLAVIGTVLIVSSAATRPFAEIRLSVFGGMGLLVVAGIFFRLSRVR"
     gene            6971..8341
                     /locus_tag="PLZ74_00850"
     CDS             6971..8341
                     /locus_tag="PLZ74_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR96903.1"
                     /translation="MSMNRRGFLKNAAWAGGAVGVGAWSGCMSSSGISARKGGSMAGF
                     RCEPMATVRVGVIGVGHRGPGAVRRLSSIPGVEIRAIGDLYEDRVAKQVKVLTDKGLP
                     APKGYFGSQDAWRGMCESNVIDLIYITTPWLWHAPMALYAMRCGKHAVTEVPAAMTVE
                     QCWQLVDTAEETRRHCMILENCCYGFNEMFALMLCRKGVLGELVHGEAAYIHDLRASH
                     LREDHENGYQGQWRLNWARQFSGNPYPTHGLGPVCQYMDINRGDKFEYLTSVSSGQFG
                     ISRAAAKKYGSNSPQAKAVYRQGDMNTSIIKTYRGRTIMVQHDTTSPRPYSRLNLISG
                     TKGILSDYPLRVALEPDPHRWLDDKKLEELKAKYTHPLWAKQGEYAKKMGGHGGMDML
                     MDMRLIHCLQNGLPLDISVYDTALWSSVVGLSVDSVARDGRPVRVPDFTRGGWKTADP
                     LEIVTL"
     gene            8415..9179
                     /locus_tag="PLZ74_00855"
     CDS             8415..9179
                     /locus_tag="PLZ74_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOR96904.1"
                     /translation="MKVEIAKSTEEMAVAAAACAAEGVRGAIARSGCARIIVATGASQ
                     FDFLKALVRAPEIDWTRVTGFHLDEYVGLPITHRASFRAYLRERFIAALPAPLAAFHE
                     VDGENPDPAAECRRLQNLLDAAPIDVACVGIGENGHLAFNDPPADFDAKAAYLVVDLD
                     EACRRQQVGEGWFASLEEVPKRAISMSIPQILKSRRIVCTVPDARKAPAVRNTIEGPV
                     SNLCPASILQTHADCTVFLDPSSAGELRSAKGVASV"
     gene            complement(9230..10375)
                     /locus_tag="PLZ74_00860"
     CDS             complement(9230..10375)
                     /locus_tag="PLZ74_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOR96905.1"
                     /translation="MRHFHVLVLMEWYDHPNRVGIGRYAAEQNWYLTVSDGCVPPKGW
                     SGNGILTLINERNDIIDYVRSQRVPCVDFGAYRPDIPLARVCGDHRRIGLVAADHLTE
                     RGHTQAAYFSTGTGHPQMLRHAGFTERFEARTGHTPLPLVWAHRPGSANDDWQSLNRW
                     LKQELHRLPKPLAVFCYCDYDAAKVENACLEAGYKIPEDVAILGVDNDTLVCENLRIP
                     LSSVRHDRVRVGYEGSALLDRLMRGGTPPTEPVLIPPRGVELRASTDGFATADPIIRA
                     VIHFFREHLDRSIGVAEAAAQAGLPPRRLEEHFTRVLGQTVYTTLTRLRMFEVKRLLA
                     FSDLPVKEIARRTGFCHAQHLNNAFVRLERCTPLAYRKSESQAASRR"
     gene            complement(10519..13254)
                     /locus_tag="PLZ74_00865"
     CDS             complement(10519..13254)
                     /locus_tag="PLZ74_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96906.1"
                     /translation="MRRAIDVLMHEKNLDTQRIAVVGVGSVAKTVLWAAANDERIACV
                     AVLGASAHEKLLQLIAPRLLYVVPCTSKNRKEAEATFLSIRRAARLWETAYGLPGLGI
                     KTYPNPDFERGDGRIGFYESATPHSITRTGWVRYLDFVDRHLGKPATAADAPCEPASP
                     FKVTVSSEPKLPVVNYVKNGDYELGFKTYVFGPGAEKIMAITNDAYEGDCALWTAKGC
                     GHSINTRGVAPPGTTNRIAITVKLGPEHKGLTGLGAGFSQSYYAAPSTGGKLVPFAAS
                     GVNLTWQQAGSYTGWQRIESEPFVVPPDATNIVMNIHAGSLCYCATGGIVDNLFCGPA
                     WTDLSFVIENTTGLAQVTVEDENGDRVFCADAFPENLTRFERTIKVSAAMRYCVKSVD
                     RKAQSIAKWYPSPRPDSAAARAPLSVKGLRRLWPSNEKAAVFLVEMRDVCELAGASLV
                     AAVTATNGVEVMRKTFAPVLMQNRIEIPITELADGCYQLVASYHDAKGAAVTAKAPVD
                     FWKFPAMAGRPRDIYTIKDRNIYKNGRFFFPIFTWGGFPGWGTLRNHRFDTNAYIRCC
                     HDAIAEQKALGFNAILTDAWYFPDLARRAFSEIACRKYFPAWIPYTQVSQIPLDFLDI
                     ARMYDERGLAITPYLHIDRPDRVDDVDAWLKAVVNYRNADNILCWHLSDELDAAIAHN
                     RMLNDLHHEADPDRLTWINFINGARNNRDNGDILSEDPYPIPNERIAKVIWHAEKVRD
                     CTRPDQSVFQWLQMFGGEGTWTRSPTIAELEGMVYMSLNHGVRGLAYFGYTYSLADRD
                     GIRSMTEADYAKLGTLNRRVERLAPIYCLGQALERVYDNNLHYDRAVFRYAGRIWVSV
                     VNGLPEARHLRIPLPQDVKRVGKAEVVDDNRQISFKDGAIEEKFLPYAVRLYRLK"
     gene            complement(13646..15844)
                     /locus_tag="PLZ74_00870"
     CDS             complement(13646..15844)
                     /locus_tag="PLZ74_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96907.1"
                     /translation="MKRLFLFLFVLVLAVAGVVQAVETVAVPVLDSIPSLVDGMLTDD
                     CWSKALTLAPFTPIQPNQKPLVQKTEAKLFVTKDAFYLGLIGYTEDAAFTHRQSTDVK
                     AGPFGVDHFEIFVDPGHSGNLMQLAFGVNGCVYGNTSGRIRFGVQVYDDRYQIEVEIP
                     FDIFPLKRQGFNSEWLFNVARDAKLAGVDVCAGEYSSWSQLVSGTFHEADSFNRLTGI
                     GYNLAASFALQAAATKKPIELVAEKHLWMSDTVRIIAETNGKVPFVEISCAVSNSTGS
                     VVWQGKVKATGTKAAIEIPLKGCADGRYRFEAKFVDASGRTLVETHGVDFWKIPPKKT
                     VPRTIFTCRDNNIYKNGEFFFPIITWGGAPEWGVLRNHRFDTNAFFRCYHAILAEQKE
                     LGFNTILTGAEYYPDLARRALTDKTCRKYFPAWKDYRKIAQLPFDFNESIALHAEHNL
                     AIIPYLHIDKPQNAEDVESWLGSVLKWREADNILCWHLSDELDGAIDHNRMLNELHHD
                     VDPDRLTWINCIQGIAANRDNADILSTDPYPIPHSTVTMVGFHVKRLMDTCRPEQSPW
                     LWLQMFGGEGNWTRSPTFAELKSMVYITLNRGVRGIAYFIYKPDLVDRDGIRSITQED
                     YDKLGPMNRRVARLAKMYCLGEVIERQADDRKLTDVAVIKYDRSFWVSVVNGNTAKQR
                     VRVKLPVSLPQSGIADVDDENRTVSFQNSVIEEEFEPFAVHVYKIRHIAQ"
     gene            complement(15846..16463)
                     /locus_tag="PLZ74_00875"
     CDS             complement(15846..16463)
                     /locus_tag="PLZ74_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96908.1"
                     /translation="MMKKPMITLVALVVAIAAQAAELPLGTDTSRNYLKNGSFEIGRD
                     NFDYCGWQNNDPTGWSFSITNAASAGKRALFVEKAGSYLSQHAYVPGGTYVYTVTIRA
                     NPETDFQLGKGKPPKGLGMSYGANFRMEKGGKETRLGGHLLGGCPKDGTPAWQRLVSA
                     PIIIPTNIYGNCYMHIQCGVFHVPKGTGLIDDVALYRVNAPTDKK"
     gene            complement(16503..18518)
                     /locus_tag="PLZ74_00880"
     CDS             complement(16503..18518)
                     /locus_tag="PLZ74_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96909.1"
                     /translation="MKRLLIGLLAVSVFASSASSTVIYVSPYGSDETGDGSLENPYRT
                     PTYVIDNDSGRKVTTGCTVCILPGTYASVKSAYTDVKITVRGLHEPIGPNGETEPIID
                     GGYPLTEHRIFYLNNADIVVENLTFRNCCARYPGNQDGSAILGKFAAVRNCTFTNCVM
                     REAYHGGAISAQGTCEISNCTFVACKSIEPAYRNCSALFVSSGTATLVDCKFKYCTSV
                     GNSSGTACLSATGVVKNCTFEHNLARTQAGLYIGGGDYDISDCTFVDNVATEGSGAGI
                     TIDGTGAAGGRIVRCTFNGSKAAGNCALSVSGAPAWIENCTVSNAVAGFATCLAVTAP
                     ATVTNCVFTDCFSTNGGTTVQAKTSEQTFIDCIIADNRVEYRKNGEINTDYGAGTGVW
                     FSGGKKVFRRCSITNNYTTCWQAGGIYGEVTSEWYDCLVSSNSAGYYGGGTIRGNGTI
                     VSNCVFSENVARSGNCGGLYIQNGSDVLITDCAFIGNKAPTSIAGALTAPAGMTCRNT
                     LIVRNVSKNEPGGASIPKNSRWENCTVVGNVSSSGMCNLQSGDGAGSVFVNCLIAKDL
                     VSNAAGFWCGGSHTLTSCILASTDLSNALTEGSTYQVVYGAALKLGEDYRPICQSSPC
                     VDAGTDELAWIAGAKDLGGRHDRKIGEHVDIGCYEYKPWIGTTLYLK"
     gene            complement(18571..21621)
                     /locus_tag="PLZ74_00885"
     CDS             complement(18571..21621)
                     /locus_tag="PLZ74_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR96910.1"
                     /translation="MHKSVFCGVFVAMLFASETWARTITCEGVKTDGVVTSFNLTFGA
                     SADGKTLGLYCAYGSEDCGSDPAAWPDLRRWATVPSTVNVLGAMLPPNWGVSVFALRF
                     FLFEEENASLGYDSRVDYLQSTGVQVIDSTYRANGNSRFVLEMTGMPSPLHSEGFVFG
                     YRSTHCAPDSFGLYQHCGKNHWKTPGWRYGTQIFTGSVDTNAHHIQNRCIVSNDLSRI
                     YVSQNGVATCINPDAPDDCGFTGSWNLAIFGAYYTSNRDTTGLVQTRIHSLSFYEGDT
                     AQREFIPVLKDGVPGLWDNVNGQFYPKTAGSADFLYGERLDNPVGATTFVSSSEPWVR
                     AEPNALFNIAASGMAIHGFGTLYTCGDGNNEVDVYLACAKSTEELPAPTLVWQSWKDD
                     LDTRFNTMIKGLEPSTAYNYSAYVMNPAGVKSLVTNGTFTTGPIIEVAVNEDPVSAVN
                     AAAAGSEVRIAEGTYVISGTMAPKASVAVVGQGAGAVLDANFVVDRRHVYTSVAHVTL
                     DNLTFKNARHETHTGYANWVHCGAAVRFGNNGGNSTSSQYAWITNCLFSANTNVNEGA
                     GGLFMECANKVVDCVFENNYGGPHPTSGLTGCGAAFVHHGNDYSQFSHCIFKDNDSGS
                     YGVVGSGTYTSGSALGMSRYGFVCNDCVFTNNTSTSGGILAGKISMVKRCEFIGNRSA
                     NGGVCWAGYPDSRWITNRFEQCVFRNNSASARGGVIASDYWRYFVFTDCAFIGNVAAT
                     AGSVFRGNGDFLCTGCLFTGNDSTSATLGNVADANGTMIAAQVPRLYDCTVSNNTATG
                     YSLVYVYNNADGGKFHNTRFIANLLSGADGTDIQRGVLWPHKASEVRNCLFAVNTNAC
                     GYGCAIALYSNSNEVDNCTVTGNRCYSEGNSGCVGIYRNTGTENRIRNCIVTDNRNLT
                     QDNLLHNFYGWGIDQVSYSLEDGTQLPNGVRGNINEADPRFVSPEKGDYSLFCRSPAR
                     NAGVNDDWMLGAKDLAGQDRILDDVVDMGCYEYRTIPGFMIRLH"
     gene            22110..22271
                     /locus_tag="PLZ74_00890"
     CDS             22110..22271
                     /locus_tag="PLZ74_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOR96911.1"
                     /translation="MQRYVCNVCGYVYDPEVGDPSNGIAPGTPFEALPEDWCCPDCGV
                     GKDQCEPAT"
     gene            complement(22350..23255)
                     /locus_tag="PLZ74_00895"
     CDS             complement(22350..23255)
                     /locus_tag="PLZ74_00895"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOR96912.1"
                     /translation="MRYDQNHALLESSIPGLPPPKRGKVRDLYDLGDRLLLVATDRIS
                     AFDVVMPNGIPDKGRILNGLSVFWFSLLTETANHLITADVDAYPEPLQAVKEHLRGRS
                     MLVRKVSIIPFECIVRGYLTGSGWKEYQTHRTVNGQPLREGYRNGSKLDKVLFTPSTK
                     AEQGAHDMPVSAAELEAALGAEKAGRLAAASIRCYTKAAEYAAQNGILIADTKFEFGE
                     EGEGQLVLADEVLTPDSSRFWPQDQYRVGSNPPSLDKQFVRDWLESVRFNHQPPAPVL
                     PDDVVARTREKYVEAYERLTRRRFG"
     gene            23676..24101
                     /locus_tag="PLZ74_00900"
     CDS             23676..24101
                     /locus_tag="PLZ74_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR96913.1"
                     /translation="MNLNKVLSEAITKGATKVIRKPIAGIRRDVAQLKREIAELKRLI
                     RNAGRRQPSVAAARTTSAASEVPAMRPTAKMIRGLRKKLGVTQAQFAKLAGVSSLTVS
                     KWEAAPGRIRMRGHTLAGLAAVRAMGKREAKAALSKLES"
     gene            24172..24822
                     /locus_tag="PLZ74_00905"
     CDS             24172..24822
                     /locus_tag="PLZ74_00905"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOR96914.1"
                     /translation="MLRYTVKRVMPTSVASVAFGTAEKGRIGHVRPESSGHRPETDFR
                     VVHDGERLYVRFDVRDHYVRSVQTAYQAPVCTDSCVEFFVQPRPDKGYFNFEVNAGGT
                     LLLYYISDAARVEGGFRTFVKVPEEWGRQVEVRSSLPRVVEPERVGPLTWWVTIGIPF
                     ALFRAFVGGVSLSGAVWRANFYKCGDKTSHPHWLSWSPVPELNFHLPEAFGELALE"
     gene            complement(24824..25558)
                     /locus_tag="PLZ74_00910"
     CDS             complement(24824..25558)
                     /locus_tag="PLZ74_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96915.1"
                     /translation="MLRLLRLIAGVLLLPLCVAVTLALLDTLRSIPDTGVLLSAETRT
                     LLAGFFLWLGMWFFLPQPFRAYVVAHELTHAFWGALFGARVSDLRVSERGGSVRLSRS
                     NLLITLAPYFFPLYTILVVLLRLLAGLFLNPMPCPLLWLFFVGFTWCFHITFTLQSLM
                     TRQPDIQEYGRIFSYAVIYLFNLAGVGLWVVCTTTASFDTLATALALHTRTVCQQINQ
                     TVLLCSRQVYALLLPALKRLWIRPAA"
     gene            complement(25571..25867)
                     /locus_tag="PLZ74_00915"
     CDS             complement(25571..25867)
                     /locus_tag="PLZ74_00915"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOR96916.1"
                     /translation="MNILLKIITRTTLFTAFLCVFAAGVIIIPPKMAQFKRLEQQRNE
                     LLRRIDHKKAEIKVLKEKQQRFKTDPEFVEAVARQNKRVRPGEMVFVFDSEDAK"
     gene            26195..29188
                     /locus_tag="PLZ74_00920"
     CDS             26195..29188
                     /locus_tag="PLZ74_00920"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96917.1"
                     /translation="MKKRFVYGVGAWVLAGAVLAGPRVRVEEELRRNLAVTAAGAVTE
                     AHVTALTFTPQETKRVRLVIRSTQGGEAGVDELEVFGPVGTNNLALAARGVRASASSV
                     IKGYAIHATAHLNDGLYGNDHSWIAGGTGEEWVEIELPAPASVARVAFSRDRTGRYTD
                     RQPREVEVRLSKEGRAWETVCKHTGSAQVLPTLMLPASALSEPTWDGVVTYAFLCERE
                     TWGTLDGKDELSPLRNDRPAVPGAEPYWGKLARLPHVARNLTQFGGLITRLAEQGLDV
                     TAERKEAETLRARLQVEGETDALYLAVRHAKRRLFFRDPRLAPVERVLFAKRHPLQPS
                     HNYSEHMDGKFTSGGGICVLHVPRDAAGRLDPARAEVETLFDGNDGIVRHPVSDFDAG
                     TVFFVYRPDKPEVKGWHSYWHLWSVNADGGGARKLTEGPFHDFDPVALPDGDLGFITT
                     RCAARFLCWQPQAYVLYRMNRHGGEMQRLSFANLSEWDPVMMRDGRILWTRSEYQDKG
                     ADFGHTLWSIRPDGTHPELVFGNNTPYGYGHAREVPGSAEIVCTLISHGDHQGPIALL
                     DRSKGLYEVAAITSITPDTRPQYQMGRSHHQTFRFPEPVSRDHFLVSHSPGRRSHWGL
                     YLIDRYGNRELLYLDPLISSKKPSLFRPRPRPPVMPPMRDAELAAQELGQFTVQDVYE
                     GLGPTVPRGRVKYIQVSQEMPAMLEQLSCGQYRNTHGPFMDFYATPVHKVKGPSHEIV
                     TRTRNALGAHAFRQGSAVTREDGTFLVTERGGWPSFVAKSVLGTVPVQADGSANFTAP
                     AGKVLYFHLLDEHFNEIQRMRSVLQLQPGERRSCVGCHEDRLQLPAMRQTTEALAQPV
                     RALDPSAWGGGPFDYAAVVQPVLDRRCVSCHDGMKKGRPDLRGTPDADFVPTSYRTLI
                     SGGWVHYFDWIWGARHFKAEPLTFGTLRSRLFDALKKEQHREVRLSAEERRTLTAWID
                     LNCPLWPDYRYRPERSGAPLQASVR"
     gene            complement(29243..30784)
                     /gene="hydG"
                     /locus_tag="PLZ74_00925"
     CDS             complement(29243..30784)
                     /gene="hydG"
                     /locus_tag="PLZ74_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOR96918.1"
                     /translation="MTAQTLTSAPLRRFANAADYRKRFIIREQIDRYLPGGRHYLDEA
                     ELERLLAAASAAPANDPVRVREILAKSRAIETLLPEETASLIQVTDPALLDEMETTAL
                     AIKHAVYDNRIVMFAPLYMSNLCVNRCAYCGFRCGNEDQKRRMLTMDEVRQEVDVLAG
                     KIGHKRLIAVYGEHPDTSTEYFAETIRTVYAHQVKSPQGTPVGIRRVNVNAAPLPIED
                     LRVLKQVGIGTFQVFQETYHRATYARLHPADTVKGDYDWRTTCFHRALEAGVDDVGMG
                     VLYGLYDWKFELLASLCHARDLERQFGIGPHTISFPRLEPASGTDLPTTSPYRIDDAT
                     LRRILIITRLSVPYTGMIVTCRERPESRDAFVRCGITQMDASSNIAIKGYSDFASETH
                     QEKHRQQFVLSDTRTLEELIAHLAREGIITSFCTAGYRCGRTGGCIMDALKTGKEGKF
                     CKINAVLTFREWLDDFASAATRMLCEPLLRRELAEIETTLPKFYPTVKEHYDRIANGE
                     RDLFF"
     gene            complement(31003..>31316)
                     /locus_tag="PLZ74_00930"
     CDS             complement(31003..>31316)
                     /locus_tag="PLZ74_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96919.1"
                     /translation="RYRGRYRYRNRSFNHGTLSWVSGFQGSIQIGIGVGIAIGIDHLT
                     TEHSLGFLVSRFLVSGFWVSGFQGFRVSVFQGSIQIGIAIGIAIEIDCFTTPPTLTTR
                     I"
BASE COUNT         6256 a         9033 c         9810 g         6217 t
ORIGIN      
        1 cccgcgcgca ggccgtcagg cgctgcggag cggggggttt tgcaagagcc tcgttaagtg
       61 tatcctttat ttggagcctg ccttgatcac cggcatgtga ccgtaaagta actgaaggga
      121 gggaacatga aagcgttcct gaatggttcg gttgttttac tttgttttgc tttcgctttg
      181 ccgcgacggg gtgacgccga accggtcttg ctgttcagcg acacgttcga ttacgtcacg
      241 tccggttccg tttcaaatcc gagcagttcc acatggaaat cgtcagaact ctattcgatg
      301 tacacaaaca tctactctgc cggagggagt gtcaaactcg gcaaatcaaa tgagggcggt
      361 attctgacca ccaccaatct ggccgtgacg gcaggtctcc tgtcggttca ggttgacgcc
      421 ttgggctggg atagcgatga gaaggggttc atggtttcgg tcgggaacat gaccaccaac
      481 ctgacctgca caaacgacaa gtccgcctcc acgctgttcg agacggtgtc cacaacattc
      541 gaagttgaga ccggagtcat ttttgtaacg ttccagaccg aaaacggcaa gcggatcttt
      601 ctcgacaaca ttcagatcac ccatacgccc gaaggaggac cggtcccgtt ggcgttcttt
      661 gtttaccccc attccgccac gaccgtgccc gcgctcacgg agatcgcgtt cacgatcacg
      721 gccttggccg acggcgttgc caccaacgtg tcgtacgctg gcggactgcc ggatggggcg
      781 ctctacgact ttatcggcca gcggttcgct tggacgccgg aactcaacca caccggcgag
      841 ttcgctctgg tcttcgcggc aacggatact gcggggacgg tccatcagaa aacggttccg
      901 gttacggtca cccctcttcc cttgcatgcg ccgaccggcg tcacggttac ggaactcacc
      961 tgtaaaacgg tctccctcgc gtgggatgcg gttcctgcgg catcgcacgg ttaccgggtc
     1021 tccgcctggt acgggagtac ggcacccgac atgctttccg ttgatgggga gaccttcttc
     1081 gaaatcatgg acacggggct ggtcgccgcg ccaacggggt ggttgttcca gggcgtgacg
     1141 gaaaaatact ccacagcgga tttcgtggag ttgaaattca atgataccgg cgacagtgtg
     1201 ataaccaaac gttatcccgc tccggtttcc tcgctctctt tccgcctcaa ggggtatgcc
     1261 acgagttcac tgtccaacag tcttttcacg gtgtacggtt catgtggcgg cgacgattgg
     1321 acactgctcc ggcagtacag ttcgctggag gattctgacg gagatgacga gaacaacctc
     1381 ttcaccagcg cggaggggga cctcgataaa acggtgaccc tgcatgcggc cgaaggctac
     1441 cggcaattcc gttttgtgta tgggcagaag gcgagcggca acatcgggtt ggccaacgtg
     1501 actgcggcgt acgccgggag cggagcccgt ttcgtggacg gctggcaggc aactccggtc
     1561 tccgccacga atttgcggat caccggtttg ccgcccgcgc acgaaatcct catccgtatc
     1621 gaggcgctgg atgccacgga gagccgcgcg acccaactcc gcctgtcttc tcccccgttc
     1681 ctgcccaaga ccctcctcat gattcggtga cggagtgtcc ggctccgtgc cgatggcgcg
     1741 tagcggtgga cgtcccgccg tactggcagg ggctgtctgc ggctcgcggg ggcgctcgtt
     1801 cctgccgact tgaggcatcg ggtttggaat caacggcctt gcgagtcaat cgagggagga
     1861 accaagacgc cggttttttt gcactccgca tacgtcataa tgcaattgat ggaaacgggt
     1921 cgattccgct atcctgttat gtcggagttt atgggtgcga atctgaaaaa ggggaggaag
     1981 catgaaccgc agggattttc tgaaaacaac ggtttacgcc ggagcagttg ctatgggcgc
     2041 gcgcacggcc tccgcgcagg catcgcctgc cgagcgcgtg cgggtggcgg tgatggggac
     2101 gggcggccgc ggcgcgtccc tgatgaagac ggccgcggaa ctgccgaatg tcgagatcgc
     2161 gacggtctgt gatgtggacg tgcgcgcgat ggaccgggct gccgagacgg tgagcaagaa
     2221 aaccggcaag actccgaaga aagagaaaga cctgcgtcgg attctggagg acaaggagat
     2281 tgacggtgtg atctgcgcca cgccggatca ctggcacgcg ccggccgcgc tgatgaccat
     2341 gcgggccggc aaggcaatct atgtggagaa gccctgctcc cacaatccgc gcgagggtga
     2401 gatgctggtg gaggcctcgg ttcggcacaa cgcggttttt cagatgggca accagcgccg
     2461 cgcctcaatg acctatcggc aagtcatccg cgaaatccaa ggcggactca tcggcgagcc
     2521 gcgtttcgcg cgctgctggt acaccacgca acgcggcccg atcggcaaag gcaaggcggt
     2581 gccggtgccg gagtggctgg attgggattt atggcagggg cccgcgccgc gccgcccgtt
     2641 ccgcgataac atcgtgccgt acacgtggca ctggtttttc cactggggca ctggcgagag
     2701 cggcaacaat gcgacccatt tcgtggatgt cgcgcgctgg acgttgggcg gacggttccc
     2761 gacgcgcgtc accagcgcgg gcgggcgtct cttttacgag ggggacgatt gggagtggta
     2821 tgacacgcag accgcgacgt tcgagtatcc caaccgcacg ttcatcacgt gggagggact
     2881 ctcggcggtc aaagcccgtc cttacgaggg gatcggtacc ggctgcatgg tttacggtct
     2941 caagggtgcg gtgctgttcg cgcccaacaa cacctgccgg cattttgatc cggcgggcaa
     3001 ggtggtgaaa aactgggacg cgcaatctct tgcgggagac gcgatggacc gtgcgaatcc
     3061 gatcggcggg ctcgatgcgg cccatctcgg aaactgggcc gactgcattc gtgcccggga
     3121 ccgcgcgaag gcctattcgc ctgccgatga ggcacacgcc agtacgctga tgacccatct
     3181 ggcgaacatc gcccagcgta ccggggagac gatccggacc gatcccgcga cgggtcggct
     3241 ggcccaaggc tcgcccggcg cggaactgtg ggcgcgggaa tacgagaagg ggtgggaagc
     3301 cagcgtctga tgcgcggagg ggaaaggatg agtccggtga tgcggcacct gtcgcgacgc
     3361 atgttttcag cacaggccct gctggccgca ggagggtggg ctgcaggtgt ctggcgggca
     3421 tccgccgatg ccaagcggcc actcaaggtc tacatctttg cggacatgga aggtatcagc
     3481 ggcatcaccc acagcgggca gacgcttgcg gacggtgccg cgtacggcga gggttgccgc
     3541 ctgatggagg cggacatcaa cgcctgcgtg gcgggctgtt ttgacgcagg ggcgacggaa
     3601 gtcacggtgc gcgacgggca cggccgcggc aacaacgtcg atcccgcgcg ggtcgacccg
     3661 cgcgcgcggc tggtgcaggg ccccacgccg ggggtgcgct tcgggggctt cgaaggcagt
     3721 gcggcgctga ttttgctggg ctatcacgcg aaggcgttga cgcccgaggc gctgctggcg
     3781 cactcgtatt catcgctcac gattcagggc atgtggctga acgggcgcga ggcgggcgag
     3841 atcggcgtgg atacggcgat cgcggcggag catggtgtgc cggtggtgat ggtttcgggg
     3901 tgcgacaagg ctgtggccga ggcgcgggag tggattcccg gcgtggtgac agtgcagacg
     3961 aagcgcagta cgggtgtgca gagtgccgta tgcctgccgc tggaatcggc acggcggatg
     4021 attcgggaga aggccgccga ggcggtccgc aggcggcagg cgatcgcgcc ggttgcggtg
     4081 gcgtatcccg tcacactacg catggacttt ctgccacaag gatcgctgcg cacatacgac
     4141 ccggcgttca cgccgacgtc gtcgccgcgg cgggtggaac ggacgggcga ctctgtggag
     4201 aagctgttgc tcaacaagaa ctgaggggga gaggatgagg aagacggtaa ggattgcggc
     4261 ggcgtttgtt ctggcatgcg gcgcggtgta cgcagaggtt acacaggtgg ggatcatcgg
     4321 gttggacacg tcacacacga tcgcgttcac caaaatgctg aacgtcgaga aaggcgaacc
     4381 gttgtttgaa tccttccgcg tgaccgcggc atatacctat ggcagtctgg acatcccgtc
     4441 cagcaccaac cggtatcctg cctacaccaa acagatggtg gagatggggg tcgaaatcgt
     4501 cggctccatc gccgaactgc tgaagcgtag cgatgtgatt ctgctggaga ccaatgacgg
     4561 ccggcgtcac tacgagcagg cgctggaggt gttcaaggcg ggtaagcggg tcttcatcga
     4621 taaaccgatc gccgcatcgc tcacggatgc ggtgcggatt ttcgacgcag ccaagcatta
     4681 cggcgtgccg gtcttcagct cgtcggccct gcggtactcg acaaagattc agaaggcgca
     4741 cgccggggcg tttggccgga tttccggcgc ggacgcctac acgccctgcg cgttggagcc
     4801 gacccatccg gatctgttct ggtatggcat ccatgctgcg gagccgctct tcacggtgat
     4861 gggcacgggc tgcgagagtg tggtgcgcgt gagccgggag aaggtggatt tcgtggtggg
     4921 ggtgtggagc gacggacgtg tgggcacgct gcgggggatc cgcgacaagc ccgcgcctta
     4981 cgggtgtgag atcttcgccg agaagggcgg gcgtctcgcg ttgggcggct atgaggggta
     5041 caagcccctg ctgatcgaaa tcctcaaatt tttccagacc ggccgggtgc cggtctctcc
     5101 ggacgagacg ctggagatct tcgcctttct ggaggccgcg cacgaaagta cgcggcgggg
     5161 tggcgtcccg gtgaaggtgg cagaggttct ggatcaggcg cgcgccgaag cggcgcggaa
     5221 agggatgaag tgaccatgca gcaaaacgga ctcgcctttg cggattacgc ggtggtcgtc
     5281 agcttctttg cggtgatgat ttcagtcggg gtgttcttcg gccgcagtca gaagaacatg
     5341 gcgcagtttt tcggcggcgg caagcaggtg ccctggtggc tgagcggcat ttctttctac
     5401 atgtgcagct tcagcgcgct ggcctttgtg atgtactccg cgctggccta caagttcggc
     5461 tggctgccga tcacgataag ctggttgagc gtgccggctg tatttatcgg gtgccggctt
     5521 ttcgcggcgc gctggcgtcg cgcggctgaa accagtccgc tggagttcat cgagcggcgt
     5581 tacggcaaca gcatgcgtca ggggctgatg tggctggggc ttccgatgcg cgtgctcgat
     5641 gacgcgttca agctgctggc catcggcacg gtagtcggtg cgggcatggg cttccccctg
     5701 cacggcgcaa tcatcgcctc ggggttgatc atccttagtt acaccttcat gggcggtttg
     5761 tgggcgactc tggtcgcgga cttcatccag ttcttcgtgc tgttcaccgt gatcctggtc
     5821 ctgccgttcc tctgtctgga caaagcaggc ggttggtcgg gatttgtcga gaatgtgcca
     5881 ccggggttct tccgtttgac ggcagacaag tacaactgga cttacatgct ggtctttttc
     5941 ctgatgctct tcttcaatct ttcgacatcc tggtccatgg tgcaaagata ttattccacg
     6001 cgcagcgaga aagacgcccg caaggtcggc tatttcgttt gcatactgct ctccctcggt
     6061 ccgccgcttt tcttcatccc ggccatggcg gcccgtgtct tcatgcctga aatacccgcc
     6121 gatcgcatga acgaggtgta cgcgttggtt tgcaagaatg ttttgccagc agggttcatg
     6181 ggcatggtca ttgcggccat gttctcggca accatgtcaa cgctcgccgg cgactacaac
     6241 gccgtggctt ctgtggtgac caatgatttc tacaagcgga tgatcgcccc gaacgcctcg
     6301 cccaagcagc acatgctggt cgcccgtctg gcgacgctgc tggtcgggct ggcggtgatc
     6361 gggctcactt tcgtgatgca gaacgcgcaa ggcgcaaacg acctgttcga tacagccaac
     6421 aaggttttcg gcatattcct gccgccgatc gccatgccga ccatgttggg actggtgacg
     6481 cgccgcatca cgcgccgcgg gggtatggcc ggcctatggg gaggtgtcat cgtcggtctg
     6541 gtgacgttta tcgctggcgc gcgttggccg gttcttcgtg ttaccagcgt gatctttttc
     6601 atcacctgct tcgccacgct tgccggactg tttttgggtt ccttgctgtt aaaggatgac
     6661 ccggcgcaca tggaatcgct gaacagcttt ttcgagaagg tcaacacgcc gagcccggtg
     6721 ctggagggac cgcctgcccg tatcacgttc tggccgctgg tggcgggcgg actggccgtt
     6781 atcggcacgg tgctgattgt ctcgtcggcc gccacgcgcc cgttcgccga gatccggctc
     6841 tcggtattcg gtgggatggg cctgctggtt gtggccggga tctttttccg actttcgcgg
     6901 gtgcggtaaa cgacggccgc gcggtgcggt gtcggaacca tagctcacaa accaacaaag
     6961 gaggggcggg atgagcatga atcgcagagg gtttttgaag aatgcggcgt gggccggagg
     7021 ggcggtcggc gtcggcgcat ggtcgggttg tatgtcatct tctggcatca gcgcgcgcaa
     7081 aggcgggtcg atggccggtt tccggtgcga gccgatggcc acggtccgtg tgggtgtgat
     7141 cggtgtcgga caccgcggac ccggcgcggt gaggcggttg tcgagcattc cgggagttga
     7201 gatccgcgcg atcggcgatc tgtatgaaga tcgggtcgcc aagcaggtca aggtgctgac
     7261 cgacaagggt ctgcccgcgc ccaaggggta tttcggttcg caggacgcgt ggcgcgggat
     7321 gtgcgaatcc aacgtgatcg acctgatcta catcactacg ccttggctct ggcacgcgcc
     7381 gatggcgctc tacgcgatgc gatgcggcaa acatgcggtc actgaggttc cggcggcgat
     7441 gacggtggag cagtgctggc aacttgtgga tacggcggag gagacgcgcc gtcactgcat
     7501 gatactggaa aactgctgtt acggcttcaa tgagatgttt gcgctgatgc tgtgccgcaa
     7561 aggcgtgctg ggcgagttgg tgcacggtga ggccgcctat atccacgatc tgcgcgccag
     7621 ccatctgcgg gaagatcacg agaacggcta tcagggccag tggcgcctga actgggccag
     7681 gcagttttcg ggcaacccct atcccacgca cgggctcggc cccgtctgcc agtatatgga
     7741 catcaaccgc ggcgacaagt tcgagtatct cacctcggtc agcagcggtc agttcggcat
     7801 ctcgcgcgcg gccgccaaga agtacgggtc gaacagtccg caggccaagg cggtctatcg
     7861 gcagggcgac atgaacacct ccatcatcaa gacgtatcgc ggccgcacca tcatggtgca
     7921 gcacgacacg accagcccgc ggccctacag ccgcctcaat ctgatctccg ggaccaaggg
     7981 catcctttcc gactacccgc tgcgcgtggc gttggagccg gatccgcacc ggtggctgga
     8041 tgacaagaaa ctggaggaac tcaaggcgaa atacacgcat ccgctatggg cgaaacaggg
     8101 cgagtacgcc aagaaaatgg gcgggcacgg cgggatggac atgctcatgg acatgcgcct
     8161 gatccactgc ctccagaacg gcctgccgct cgacatcagc gtctacgaca cagcgctctg
     8221 gagttccgtg gtggggctca gcgtggactc ggtggcccgt gacggaagac cggtccgggt
     8281 accggacttc acgcgcggcg gatggaaaac cgccgacccg ctggagatcg ttacgctgta
     8341 accccgaagc ggacagggga ggggcacgat gcgtggcgaa aaaggggaaa agcagagagg
     8401 aagagtacat gggaatgaag gttgaaatcg caaagagtac cgaagagatg gcggttgcgg
     8461 cggcggcctg tgccgcagag ggggtccgcg gggcgatcgc gcggagcggc tgcgcccgca
     8521 tcatcgtcgc gaccggcgcg agccagttcg attttctgaa agcgctggtt cgcgcgccgg
     8581 aaatcgactg gacccgggtg acggggtttc atctggacga gtatgtcggg ctgcccatca
     8641 cccaccgcgc cagtttccga gcgtatttgc gcgaacggtt catcgccgcg ttgcccgcgc
     8701 cgctcgcggc gtttcatgag gtggacggcg agaatccgga tccggccgca gagtgccgcc
     8761 gcttgcagaa cctgctcgat gccgcgccaa tcgatgtggc ctgcgtgggt atcggcgaga
     8821 acgggcatct ggcgttcaac gacccgcctg cggatttcga cgcgaaggcg gcgtatttgg
     8881 tggtggatct tgatgaggcg tgccggcgcc agcaggtggg cgagggctgg ttcgcctcgc
     8941 tggaggaggt gccgaaacgg gcgatctcga tgtcgatccc gcagatcctg aagagccgcc
     9001 ggatcgtctg taccgtgccg gacgcgcgca aggctccggc ggtgcggaac acgatcgagg
     9061 gaccggtctc gaacctctgc cccgcctcga ttctgcagac gcatgcggac tgcacggtgt
     9121 ttctcgatcc aagttccgcg ggcgagttgc ggtcggcaaa aggagttgcg tcagtctgat
     9181 gcggacgtaa tccgctcccc tttttccaat gcgggggcag acgcttgttt taccggcgag
     9241 atgccgcctg gctctcgctt ttgcggtaag ccagaggcgt gcagcgttcg aggcgcacaa
     9301 aggcgttgtt gagatgctgc gcgtggcaga agccggtgcg gcgcgcaatc tctttcacgg
     9361 ggaggtcgga gaaggccagt agccgtttga cctcgaacat gcgcaggcgc gtcagggtcg
     9421 tgtagacggt ctgacccagc acacgcgtga agtgctcctc cagccgacgg ggcggcaggc
     9481 cggcttgtgc ggcggcctcc gcaacgccga tgctgcggtc gaggtgctcg cggaagaaat
     9541 ggatcacggc gcggatgatc ggatcggcgg ttgcgaagcc atcggtgctg gcgcgcagtt
     9601 ccaccccgcg gggcgggatc agaaccggtt cggtcggcgg ggtgccgccc cgcatcagcc
     9661 ggtccagcaa ggcagacccc tcatacccga cgcggacccg gtcgtggcgg acgctggaga
     9721 gcgggatgcg gaggttctcg caaaccagcg tgtcgttgtc cacgccgagg atcgccacat
     9781 cctccggaat cttgtaaccc gcctccaggc aggcgttctc caccttggcg gcgtcgtaat
     9841 cgcagtagca gaagacggcc agcggtttcg gcaaccggtg cagttcttgc ttgagccagc
     9901 ggttgagaga ctgccaatcg tcgttcgcgc taccgggccg gtgggcccag acgagcggaa
     9961 gaggcgtatg gccggtgcgg gcttcgaacc gttcggtaaa ccccgcgtgg cgcagcatct
    10021 gcggatggcc ggtccccgtg gagaagtaag ccgcttgggt atgtccgcgt tcggtcagat
    10081 ggtcggctgc caccaggccg atccgccggt ggtcgccgca gacccgggcc agcgggatat
    10141 ccggccggta ggcaccgaaa tccacgcacg gcacgcgctg cgagcggaca tagtcgatga
    10201 tatcgttgcg ctcgttgatg agggtcagga tgccgtttcc ggaccaaccc ttgggcggga
    10261 cacagccgtc gctaacggtc agataccagt tctgttccgc agcgtaccgg ccgatcccga
    10321 cgcggttggg atggtcgtac cactccatca gaacaaggac atgaaaatgg cgcataccct
    10381 cagtatagga taaccgggca cgctgttcaa tgcgccggta tccggcaccg gcaccagacg
    10441 cgttgcctca caattcatga caccgaaaat gaatcgcgcc tacgaccacg ctgactacgc
    10501 agatgagaga tacatatctc atttcagacg ataaagacga accgcatacg gcaggaattt
    10561 ttcttcgatt gccccgtctt tgaatgagat ctggcggtta tcgtcgacga cttcggcctt
    10621 gccgacacgc ttcacgtcct gcggtaacgg aatcctcaga tgccgcgctt cgggaagccc
    10681 gttcacgacg gaaacccata tcctgcctgc atagcggaag accgccctat cgtaatgtaa
    10741 attgttatca tatactcgtt ctagtgcttg gccaaggcaa tagataggcg caagtcgctc
    10801 cacgcggcgg ttcagtgtgc cgagctttgc gtaatcggcc tcggtcatcg agcgtattcc
    10861 gtctcggtca gccagggaat acgtatagcc gaagtaggca agaccccgca caccatggtt
    10921 gagcgacatg tagaccatgc cctccagttc cgcaatcgtc ggcgaacgcg tccacgtacc
    10981 ctcaccgccg aacatttgaa gccactggaa gacactctgg tcgggacgcg tgcagtcccg
    11041 aaccttctcg gcatgccaga tcactttcgc gatgcgctcg ttcggaatcg gatacggatc
    11101 ttcggagagg atatcgccgt tatcgcgatt gttccgcgca ccgttgatga agttgatcca
    11161 cgtaaggcgg tccggatctg cctcgtgatg gaggtcgttg agcattctgt tgtgcgcaat
    11221 cgccgcgtcc agctcgtccg acaaatgcca gcagagaatg ttgtcggcat tgcgatagtt
    11281 gaccacggcc ttcaaccacg catccacgtc atccacccga tcagggcgat cgatgtggag
    11341 atacggggtg atggcgaggc cccgctcgtc atacatccgt gcgatatcta ggaaatccaa
    11401 agggatctgc gaaacctgcg tataaggtat ccaggccgga aaatatttgc ggcaggcaat
    11461 ttcactaaaa gcccggcgag cgagatccgg aaagtaccac gcatccgtta gaatcgcatt
    11521 gaaccccaac gctttctgtt ccgcaatcgc atcatgacaa caccgaatgt aagcatttgt
    11581 gtcgaagcga tggttgcgga gcgtccccca tcccgggaag ccgccccaag tgaagatcgg
    11641 gaagaagaac ctgccgttct tgtagatgtt gcgatccttg atcgtataga tgtcacgcgg
    11701 acggccagcc attgccggga acttccagaa atcgaccggt gccttggcgg tgacggccgc
    11761 ccccttcgcg tcatgatacg aggccacgag ttgatagcag ccatcggcca gttccgtgat
    11821 cggaatctcg atccggttct gcatgagaac cggggcgaag gtcttacgca tgacttcgac
    11881 gccgtttgtc gctgtgaccg ccgccacaag tgaggctccc gcgagttcgc acacatcacg
    11941 catctcgacc aagaagacgg ccgccttttc gtttgatggc cagagacgac gcagcccctt
    12001 gacggacaac ggcgcacgcg ctgcggctga gtccgggcgc ggactcggat accacttcgc
    12061 gatactctgc gctttgcggt cgacggattt cacgcaatag cgcatcgctg cggaaacctt
    12121 gatcgtcctc tcaaaacggg tcaggttctc cggaaaggcg tccgcgcaga agacgcggtc
    12181 accgttctcg tcctcgaccg taacctgcgc aagtcccgtc gtgttctcga tcacaaacga
    12241 aaggtctgtc caggcgggtc cgcaaaagag gttatcaacg attccgcccg tcgcacagta
    12301 gcagagcgag ccggcatgga tattcatgac gatattcgtc gcgtcggggg ggacgacgaa
    12361 tggttccgac tcgatgcgct gccaccctgt gtaagagccc gcctgctgcc atgtaaggtt
    12421 gacaccgctg gcagcaaacg gcacgagctt gccgcccgtc gatggggccg cgtaataaga
    12481 ctggctgaag cccgcgccaa gaccggtgag tcctttgtgc tcggggccga gcttgacggt
    12541 gatggcaatg cggttggttg ttccgggcgg tgccacgccg cgcgtattga tcgaatgtcc
    12601 gcaacctttc gctgtccaga gggcacagtc tccctcgtaa gcatcgttcg tgatcgccat
    12661 gattttctcc gcgcccggac cgaagacgta ggtcttgaaa ccaagttcgt aatcgccgtt
    12721 cttcacgtag ttgacgacgg ggagtttcgg ctcggacgat accgtcacct tgaacggtga
    12781 ggccggctcg cacggcgcat ccgccgcagt cgctggctta ccgagatgac ggtcgacgaa
    12841 atcgagataa cgaacccagc ccgtgcgcgt tatagaatgc ggcgtcgcgc tctcgtaaaa
    12901 cccgatgcgt ccatcacctc tttcgaaatc aggattcggg tacgttttaa tgccaagccc
    12961 gggcagtccg tacgccgtct cccagagtct ggcagcgcgt cgtatggaca gaaaggttgc
    13021 ctcggcctcc ttccggttct tcgacgtaca tggcacgaca tacagcagcc gaggcgcgat
    13081 caactggagc agcttttcat gagccgatgc gcctagaaca gccacgcaag cgatacgctc
    13141 atcattggcg gcggcccaaa gcaccgtctt ggcaacagaa ccgacgccga ccaccgcaat
    13201 gcgctgtgtg tcgagattct tttcatgcat caagacgtcg atcgcacgac gcacaccatc
    13261 cgtatcaggc gcaacacgaa ctacagcata tccacgacgc aagatcaagt ccgccggaaa
    13321 caccgcattc gcatcattac cgcagaaaat aataaccggt gcaggacaca cagtggacgt
    13381 tcgctgtgga cgcgatatct tcgtcacatc aatatcagat gtctcaatat cacccggaca
    13441 tggtgaggat atcacgtcac gcgttgacgc tgttccgaaa agattctgtt tgcaccactg
    13501 tatcgccttg tcgcgtccac cgtgctgcgg ttgttcccag tagttgatgc ggatcggctc
    13561 gtcatcagtc gcctgagtct gcggtacgag gcaaaggaca cacagggcga ttacaaagca
    13621 cttcatgata tccagtctca attgcctatt gtgcgatatg tctgatcttg tatacatgga
    13681 cggcaaacgg ctcgaactcc tcttcgatca cgctgttctg aaacgaaacc gtccggtttt
    13741 cgtcatcgac atcggcgatg ccggactgcg gcagggacac gggaagcttc acgcgcacgc
    13801 gctgctttgc cgtgttgccg ttcacgacgg acacccagaa ggatctgtcg tacttgatga
    13861 cggcgacgtc cgtcagcttt ctgtcgtccg cctgccgctc gatgacctcg ccaaggcagt
    13921 acatctttgc aaggcgcgcg acgcggcggt tcatcggtcc gagcttatcg tagtcctcct
    13981 gcgtgatgct gcggatgccg tcgcggtcca ccagatccgg cttgtagatg aagtaggcga
    14041 taccacgcac accgcggttg agtgtgatgt agaccatcga cttgagttcg gcgaatgtcg
    14101 gcgaacgcgt ccagttgccc tcaccgccga acatttgcag ccacaaccag ggggactgct
    14161 ctggacgaca ggtatccatc aggcgcttaa cgtggaaccc gaccatcgtc accgtgctgt
    14221 ggggaatcgg atacggatcg gtcgagagga tatccgcatt gtcgcgattg gcggcaatgc
    14281 cctggatgca gtttatccac gtcaggcggt ccggatcgac atcgtggtgc aattcgttca
    14341 acattcggtt atggtcgatc gcgccgtcca actcgtccga caggtgccag caaaggatgt
    14401 tgtccgcctc gcgccatttc agcaccgaac caagccacga ctcgacgtct tcggcatttt
    14461 gcggcttgtc gatatgcaga tagggtatga tcgcgaggtt gtgctcagcg tggagcgcaa
    14521 tcgactcgtt gaagtcgaac ggcaactgcg cgattttgcg gtagtccttc cacgccggga
    14581 agtactttcg gcaggtcttg tccgtgagcg cacgccgcgc gaggtcggga tagtactctg
    14641 cccccgtcag gatcgtattg aaaccaagtt ccttttgttc ggcgagaata gcatgatagc
    14701 agcggaagaa cgcattcgtg tcgaagcggt ggttccgtaa caccccccac tcgggtgcac
    14761 cgccccaagt gatgatcgga aagaagaatt cgccgttctt atagatgttg ttgtctctgc
    14821 aggtgaatat cgtcctcggc acggtctttt ttggcggaat cttccagaag tcaacaccgt
    14881 gcgtctcgac cagcgtcctg ccggaagcgt ctacgaactt cgcctcgaaa cggtaacgtc
    14941 cgtcggcgca tcccttcaac gggatctcga tcgccgcctt cgtcccggtc gccttgacct
    15001 taccctgcca gacgaccgat ccggtagagt tcgaaaccgc acacgaaatc tctacgaacg
    15061 gcaccttgcc attcgtttcg gcgatgatgc ggaccgtatc ggacatccac agatgctttt
    15121 cggcgacaag ctcgatcggt ttttttgtgg cagcggcctg taaggcaaat gatgccgcga
    15181 gattatagcc gatgccggtc agacggttga agctatccgc ttcgtggaac gtacccgata
    15241 cgagctgcga ccagctcgag tattcgcccg cacacacatc aactcccgca agctttgcat
    15301 cgcgtgcgac gttgaacagc cattcggaat tgaagccctg ccgcttcagg gggaagatat
    15361 cgaagggaat ctccacctcg atctgataac ggtcgtcata gacctgaacg ccgaagcgga
    15421 tacgtcccga cgtgttacca tagacacagc cgttcacacc gaaggcgagc tgcatgagat
    15481 tgcccgaatg gcctgggtca acgaagatct cgaaatggtc gacaccgaaa ggccccgctt
    15541 tgacatccgt cgactgccgg tgcgtgaacg ccgcatcctc ggtatagccg atcagtccga
    15601 gatagaacgc gtcctttgtc acgaacagct ttgcctctgt tttctggacg agcggtttct
    15661 ggttcggctg gatcggcgta aagggcgcaa gcgtcagcgc cttcgaccag cagtcgtccg
    15721 tcagcatgcc gtcgaccagt gacggaatcg aatcgagaac cggcacagcc accgtttcga
    15781 ccgcctgcac aacgcccgcg acagccaata cgaggacgaa caggaacagg aaaaggcgct
    15841 tcatcttact ttttatccgt tggggcattt acgcgataga gggctacgtc gtcgatgagc
    15901 ccggtgcctt tcggtacatg gaagacgccg cactggatgt gcatgtagca attaccgtag
    15961 atattcgtcg ggatgatgat cggcgcggag acaagccgtt gccaggccgg cgttccgtcc
    16021 ttcggacagc cgccaagcag atgtccgcca aggcgcgtct ccttgccgcc cttctccatc
    16081 cggaagttgg ctccatagct catgccgagc cccttcggcg gtttgccctt cccgagttgg
    16141 aagtccgttt ccggattcgc ccgtatcgtc acggtgtaaa cataggttcc gcccggaaca
    16201 taggcatgtt gactgagata ggatccggcc ttctcaacga atagcgcacg tttcccggcc
    16261 gacgcggcat tggtgatcga aaacgaccag cccgtcggat cgttattctg ccagccgcag
    16321 taatcgaagt tgtcccggcc gatctcgaac gagccattct tgagataatt ccgcgaggta
    16381 tccgtcccca atggcagttc cgccgcctgc gccgcaatag ctaccaccaa ggcaaccaat
    16441 gtaatcattg gtttcttcat catcccatct cccccttgat tttatctgtc gacaaaaaaa
    16501 gatcacttca agtacagcgt agtgcctatc caaggcttat actcgtagca accgatgtcg
    16561 acatgctcgc cgattttgcg gtcatggcgt ccaccgagat cctttgctcc cgcgatccac
    16621 gccagttcat ccgtaccggc gtccacacaa ggagaggact ggcaaatcgg acggtagtct
    16681 tcgcccagtt tcagggcggc accgtaaacg acctgatacg tcgagccctc cgttaaggca
    16741 ttcgacaaat ccgtgctggc cagaatgcaa ctcgtcagcg tatgcgaccc tccgcaccag
    16801 aagccggctg cgttgctgac gaggtctttc gcaatcaggc aattcacgaa aacactaccc
    16861 gcgccatcgc ccgattgaag gttacacatc ccgcttgaac tcacgttccc cacgaccgta
    16921 cagttctccc agcggctatt tttggggatg gaggcaccgc cgggttcatt tttgctgacg
    16981 ttgcgcacaa tgagcgtgtt gcggcaggtc atccccgcag gcgcggtaag cgctcctgcg
    17041 attgatgttg gcgctttatt accgataaag gcgcaatccg taatgagcac atcgctgcca
    17101 ttttgtatgt aaaggccgcc gcaattaccc gagcgggcga cgttttccga gaaaacgcaa
    17161 ttcgacacga ttgttccgtt tccgcgaatt gttccgccgc cataataacc ggcactgttc
    17221 gaagagacga ggcagtcata ccattcggac gtcacctcgc catagatgcc acccgcctgc
    17281 cagcacgtcg tgtagttatt ggtaatcgaa caacggcgga aaaccttctt accgccactg
    17341 aaccaaacgc cggtaccggc accgtagtcc gtattaatct caccgttttt tcggtattcc
    17401 acacgattgt cggcaataat acagtcgatg aatgtctgtt cggatgtctt cgcctgaacg
    17461 gtcgtaccgc cgttagtcga gaagcaatcg gtgaagacgc agttcgtcac cgttgccggc
    17521 gctgtcaccg ccaaacacgt cgcaaaaccc gcgaccgcat tggaaaccgt gcagttctcg
    17581 atccaagcag gtgccccgct gacggatagt gcgcagttac cggccgcctt gctgccgtta
    17641 aacgtgcaac gcacgatacg ccctcccgcg gctcctgtcc catcgatcgt aatgccagcg
    17701 ccagagcctt cggttgcgac attgtcaacg aacgtacagt cggaaatatc ataatcgccg
    17761 ccgccgatat aaagacccgc ctgcgtacgc gcaaggttat gctcgaaggt acagtttttc
    17821 actacgccgg tcgccgacag acaggctgta ccgctggaat tgccgacgga cgtgcagtat
    17881 ttgaacttac agtcaactaa ggttgcagtg cccgaactca caaacagggc ggaacaattg
    17941 cgatacgcag gctcgatcga cttgcaggcg acaaaggtgc agttcgatat ctcgcacgtc
    18001 ccctgcgcgg aaatcgcacc gccgtggtac gcctcgcgca tgacgcaatt cgtaaaggtg
    18061 caattgcgta cagcggcgaa tttgcccaga atcgccgaac cgtcctggtt gcccgggtag
    18121 cgggcgcaac agttacgaaa cgtcaaattc tcaacaacga tgtcggcatt gttcagatag
    18181 aagatccgat gctcagtcaa aggatagccc ccatcgataa tcggctcggt ttcgccattc
    18241 ggaccgatcg gctcatgcag cccccgaaca gtgatcttca cgtccgtata agcactcttc
    18301 accgacgcat aagtacccgg caaaatgcaa accgtgcatc cggtcgtgac tttgcgcccc
    18361 gaatcattgt cgatgacata tgtcggagtc cgatacggat tctcgagcga accgtcgccg
    18421 gtctcatcgc tgccgtacgg cgagacgtaa ataactgttg atgaggcgga agacgcaaac
    18481 acactgaccg ccaataaacc gataagtaga cgtttcacac taatttctcc tgtttactcc
    18541 ccactcactc attgggcgcg cacggtccgt ttaatgcagg cggatcatga agccggggat
    18601 ggtccggtac tcatagcagc ccatatcgac gacatcgtca aggatgcgat cctgtccagc
    18661 cagatccttt gcccccagca tccaatcgtc gttcacgccg gcgttgcgcg cgggggaccg
    18721 gcagaagagc gaatagtcgc ccttctccgg tgagacgaac ctcggatccg cctcgttgat
    18781 attgccgcgg accccgtttg gcaattgcgt gccgtcctcg agcgaatacg agacttgatc
    18841 gataccccac ccatagaaat tatgcaggag gttgtcctga gttaggttcc ggttgtcagt
    18901 gacgatgcag ttgcggattc ggttttcggt gccggtgttg cggtagatcc cgacgcaacc
    18961 cgagttaccc tcggaatagc agcgattgcc ggtgaccgta cagttatcga cttcgttgct
    19021 gttgctgtag agcgcgattg cacagccgta gccgcaggcg ttcgtgttca ccgcgaaaag
    19081 acagttccgg acctccgagg ccttgtgcgg ccagaggaca ccgcgctgga tatccgtccc
    19141 gtcggcgccg ctcagcaggt ttgcgatgaa ccgcgtattg tggaacttgc cgccgtcggc
    19201 gttgttgtag acgtaaacga gactgtaccc ggttgccgtg ttgttggaga ccgtgcagtc
    19261 gtacagccgc ggcacctgcg ccgcgatcat cgttccgttg gcatcggcca cgttccccaa
    19321 cgtcgccgac gtggagtcat tgccggtgaa gaggcagccg gtgcagagga aatcgccatt
    19381 cccgcggaaa accgagccag cggtggccgc gacattgccg atgaacgcgc agtcggtgaa
    19441 gacgaaatag cgccaatagt cagaggcgat caccccgccc ctcgctgacg cggaattgtt
    19501 ccggaagacg cattgctcga accggttggt gatccagcgc gagtccggat accctgccca
    19561 gcagacccct ccgttggcgg agcggttccc gatgaattcg cagcgcttca ccatcgaaat
    19621 cttgccggcg agaatccctc cggaagtgga ggtgttgttg gtgaagacgc agtcgttgca
    19681 gacgaaaccg taccggctca tgccgagagc actgccagac gtgtacgtcc cgctccccac
    19741 gacaccgtag gagccggaat cgttgtcctt gaagatgcaa tgggagaact gggagtaatc
    19801 attgccgtga tggacgaacg ccgcaccgca gccggtgagg ccgctcgtag gatgcggacc
    19861 gccgtagttg ttctcgaaga cgcagtccac gaccttgttc gcgcactcca taaagagtcc
    19921 gcccgcgccc tcgttcacat tcgtattcgc ggagaagagg cagttggtga tccaggcgta
    19981 ctgcgacgaa gtggaattgc cgccgttgtt gccaaaacgg accgccgcgc cgcagtggac
    20041 ccagttggcg taaccggtgt gcgtttcgtg gcgcgcgttc ttgaaggtta gattgtcgag
    20101 cgtcacatgc gccaccgagg tataaacgtg acgccggtcg acgacgaagt tcgcgtccag
    20161 caccgcgccc gcgccctgcc ccacgaccgc gacgctcgcc ttcggcgcca ttgttccgga
    20221 aatgacgtag gtcccttctg cgatgcgtac ttccgaaccc gccgctgccg cattgactgc
    20281 ggacaccggg tcttcgttta ccgcgacctc gatgatcggg ccggtcgtga aggtgccgtt
    20341 cgtcaccaga ctcttcaccc ctgcgggatt catcacatag gcggagtaat tgtaggcggt
    20401 cgagggttcc aagcctttga tcatcgtatt gaagcgcgta tcgaggtcgt ccttccagct
    20461 ttgccagacg agagtcggcg cgggaagctc ttccgtcgat ttcgcacagg caagatagac
    20521 atcgacttcg ttgttgccgt cgccgcaggt ataaagcgtg ccaaagccgt ggatcgccat
    20581 tccgctcgcg gcgatgttga acaatgcatt cggctccgca cgaacccagg gctcactcga
    20641 agagacgaac gtcgtcgcgc cgaccggatt gtccagacgc tctccgtaaa ggaaatcggc
    20701 gctacccgca gttttcggat agaactgacc gttcacattg tcccagagcc ccggcacgcc
    20761 gtccttgagg actggaatga attcgcgctg cgcggtgtcg ccctcgtaga acgaaagcga
    20821 atggatgcgc gtctggacga gtccggtggt atcccggttg ctggtataat acgcgccgaa
    20881 gattgcaagg ttccacgagc cggtgaagcc gcagtcgtcc ggcgcatcgg gattgatgca
    20941 ggtggcgacg ccattctgcg acacatagat ccggctcaga tcgttgctga cgatacaacg
    21001 gttctggata tgatgagcgt tcgtgtcgac gctgccggtg aagatctggg tgccgtaacg
    21061 ccagcccggc gtcttccagt ggttttttcc gcaatgctgg taaagaccga acgaatccgg
    21121 cgcgcaatga gtcgaacggt aaccgaagac aaaaccttcc gaatgtaacg gagagggcat
    21181 tccggtcatc tcaagaacga aacgcgagtt cccgttcgcc cgataggttg agtcgatgac
    21241 ctgcacacca gtggactgga gataatcgac gcgcgaatca tagccgagcg aggcgttctc
    21301 ctcttcgaag aggaagaagc gaagcgcgaa aacactcacg ccccagttcg gaggaagcat
    21361 tgcgccaagc acattgacgg tcgaaggcac ggtcgcccag cgtctgagat ccggccatgc
    21421 cgcaggatcg ctgccgcaat cctcggaacc gtacgcgcag tagaggccga gggtctttcc
    21481 gtctgccgac gcaccgaatg tcagattgaa cgaggtgacg actccgtcgg tcttcactcc
    21541 ttcgcatgta atcgtgcggg cccacgtctc cgatgcgaac agcatggcga cgaacacacc
    21601 acagaacaca cttttatgca tacaaccctc cttttgttga aaatcagtac gtgaggcgga
    21661 cgagaccatg gggaggccgg aacgcgggca ttacagtaat ggtcacgttt accataatga
    21721 taaggtactg catttcccgt cgtgggtcaa cctgcggata acgcaatctc aaaggctgtg
    21781 agactgtgag gcggtgaggg tgtgaggctg ttaggctgtt aggctgtgag gcggtgaggc
    21841 tgtgagggtg tgaggctgtg aggcggtgag gctgtggtta aatggtcgat cccgatagcg
    21901 atagcgatcc cgaccccgat ttggatggaa ccctgaaacc ttgaaaccag gaaccctgaa
    21961 acccagaaac cagaaaccag aaaccagaaa ccctgaaacc aaagagcgtt gctttcaacg
    22021 ctctttttag gtcatggcag gaatgcgcga atccggttga ccggaatgag aaaagaaggt
    22081 taacttacga cccgaaagga gcgtgcgata tgcagcgata cgtgtgcaac gtgtgcggtt
    22141 acgtgtatga ccctgaggtg ggtgacccat cgaacgggat tgcgccggga actccgtttg
    22201 aggctttgcc tgaggactgg tgctgtcctg attgcggggt aggcaaagac cagtgtgaac
    22261 cggcgacgtg aggcgcgggc agactgaggt cggcaacaac ggtgaggcga cgggtttccg
    22321 tcgcctcacc ccgttttatc gggacgggtt catccgaagc gtcgccgggt cagcctctcg
    22381 tacgcttcca catatttctc gcgggtgcgc gccaccacat cgtccggcag gaccggcgcg
    22441 ggcggctggt gattgaagcg cacgctctcc agccagtcgc ggacgaactg cttgtccaga
    22501 ctgggcgggt tgctgccgac ccggtattgg tcctgcggcc agaaccggct cgaatccggc
    22561 gtcaacacct cgtcggctaa cacgagctgg ccttcaccct cttcgccgaa ttcgaatttg
    22621 gtgtccgcga tgaggatacc gttctgcgcc gcgtactctg cggctttcgt gtagcacctg
    22681 atcgaggcgg cggccaggcg tccggccttt tccgcgccca gcgcggcttc cagctctgcg
    22741 gccgagaccg gcatatcgtg cgcaccctgc tcggctttgg tgctgggggt gaagagaacc
    22801 ttgtcgagct tcgagccgtt ccggtacccc tcgcgcaacg gttggccgtt gacggtgcgg
    22861 tgcgtctgat actctttcca gcccgagccg gtgagatacc cgcgcacgat acattcgaac
    22921 gggatgatcg aaacctttcg aaccagcatg gagcggccgc gcaaatgctc tttcaccgct
    22981 tgcagcggtt ccggatacgc gtccacatcc gcagtgatca ggtggttggc ggtctcagtc
    23041 aggaggctga accaaaagac gctcagaccg ttcaggatac gccccttgtc cgggatgccg
    23101 ttgggcatca cgacatcaaa ggcgctgatc cggtctgtgg cgaccagcaa caggcggtcg
    23161 cccagatcat acaggtcacg cactttaccg cgtttcggcg gcggcagacc cggaatcgaa
    23221 ctttcgagca aagcatggtt ttgatcgtat cgcatagaca cctgctggtt aaatgacgca
    23281 cagtttagcg taaaaaccgg ttaaatccag tactggattt gaaaaagaat ccgggttcgc
    23341 cccgttcgcg ttttcgtggg cggatatgga tcaagcgttc aatcttaaga taaggcaagc
    23401 cgggatttcc gacagcttcg cttcggcggg aagaaaaccg ggattaacag aatttatcat
    23461 tgaggtcata gacgaggatg ggttgtcgaa acacgcaacc tcaacgtgca atgactcgga
    23521 tgcgccccgt taccctcggc gtatgaaaag cgaaaaagcg aaaatcacgg acttgacgca
    23581 gagggggggg taaaggtatg atataagttt gttggccttg ctggccgtta ggcatacgtt
    23641 gccgataata gatcacctca aaaacggagt accccgtgaa tctgaataaa gtcctatctg
    23701 aagccatcac gaaaggcgca accaaagtga tccgtaagcc gatcgctgga atccgtcggg
    23761 acgtggccca actcaagcgc gaaatcgcgg aactgaagcg gttgatccgg aacgccggac
    23821 ggcggcagcc ttctgttgcc gccgcacgga ccacctccgc cgcaagcgaa gtgccggcca
    23881 tgcgccccac agccaagatg atccgcggtc tccggaaaaa actcggcgtt acgcaggcgc
    23941 agttcgcgaa actggccggg gtctccagcc tcacggtttc gaaatgggag gcggcgcccg
    24001 gacgcatccg catgcgcggt cacacccttg ccgggctggc cgccgttcgg gccatgggca
    24061 agcgcgaggc gaaagcggcg ctttctaaac tcgaatcctg atattccgaa accgacatgc
    24121 gcgcgcgctg gcgggatgtg ccggcgcgac ggacgcgcgg gagaggcggg tatgctgcgg
    24181 tacacggtga aacgggtgat gccgacatca gtggcgtcag tggctttcgg gacagcggag
    24241 aagggccgga tcgggcatgt gcgtcccgaa tcctcgggcc accgtcccga gacagacttc
    24301 cgggtggtgc atgacggcga acgtctgtat gtccgctttg acgtgcggga tcattacgtg
    24361 cggtcggtcc agaccgcgta ccaggcgccg gtctgcacgg acagttgcgt ggagtttttt
    24421 gtgcagcccc gtccggacaa ggggtatttc aattttgagg tgaacgcagg gggcacgctg
    24481 ctgctgtatt acatctcaga tgccgcgcgg gtggaaggcg gcttccggac gtttgtgaaa
    24541 gtgccggagg agtgggggcg gcaggtggag gtccgctcct ccctgccgcg cgtggtcgag
    24601 cccgagcggg tgggtccgct gacgtggtgg gtgacgatcg gaatcccgtt tgcgctgttt
    24661 cgggcctttg tgggtggggt ctcgctttcc ggcgcggtct ggcgtgccaa cttctacaaa
    24721 tgcggggaca agacctcaca cccgcactgg ctctcttggt ctccggtccc ggaactcaat
    24781 ttccatctgc ccgaggcgtt cggtgaactg gcgctggagt gactcaggcc gcaggtcgga
    24841 tccacagccg tttcagggcc ggcaacagca acgcatacac ctgccgggag cagagcagaa
    24901 ccgtctgatt gatctgctgg cagacggtcc gggtgtgtag cgccagcgcc gtcgcgagag
    24961 tatcgaaact tgcggtggtg gtgcagacca cccagaggcc cacccccgcg aggttgaaga
    25021 ggtaaatcac ggcgtaggag aagatccgcc catactcttg gatgtccggt tgccgggtca
    25081 tcaggctttg gagcgtgaag gtgatgtgaa aacaccaggt gaaccccacg aaaaagagcc
    25141 agagcagagg gcagggcatg gggttcagga aaagtccggc gagcaggcgc agcaggacca
    25201 ccagtatcgt gtagagggga aagaaatagg gcgcgagcgt gatcagcagg ttgctccggc
    25261 tcagacgcac gctgccgccc cgttccgaga cccggagatc gctgacccgt gcgccgaaca
    25321 gcgcgcccca gaacgcgtgg gtgagttcgt gcgccaccac gtaggcgcgg aaaggctggg
    25381 gcaggaagaa ccacatgccg agccagagga aaaatccggc cagcagggtg cgcgtctccg
    25441 ccgacaacag cacaccggtg tccgggatgc tgcgcagggt atcgagtaaa gcgagcgtca
    25501 cggccacgca tagcggcagc aacagcacac cggcaatcag tctgagcaga cgcaacatgc
    25561 cgtcgcgatc ctacttggca tcttctgaat caaacacaaa caccatttcg ccgggacgca
    25621 cccgcttgtt ctgccgcgcg acagcttcga caaactccgg gtcggtcttg aagcgttgct
    25681 gcttctcttt cagcaccttg atctcggctt tcttgtggtc gatccggcgc aacagctcgt
    25741 tgcgttgctg ctccagccgt ttgaactgcg ccatcttcgg cgggatgatg atcacccccg
    25801 ccgcaaacac gcataaaaat gcggtgaaga gcgttgtccg ggttatgatt ttgagcagaa
    25861 tgttcacgcg gtcagcccgt aggtcgcaat ccggttgttt gattgtgagt actcaatcgc
    25921 atattcgctt aaaagtcaat cgcgaatttt ccgtaatcgc caaattgtgc gattcgacgg
    25981 ttgacgcaaa aaaggcggtg cggtaccgta tttccaccgt tgggaatcgc ttttgcagga
    26041 taaagtcgtg taatagggcg ggaagagagc gcatgcggac ttaacacgac gttaccggta
    26101 ggggggacga gaggtttgga aacccatgac gaagaaaaac gtcaaaagat cggttgcggc
    26161 gcgaacgggc agcgatcacc gcgcggggat aggcatgaaa aaacggtttg tgtacggggt
    26221 gggcgcatgg gtgttggcgg gagcggttct ggccgggccg cgcgtacgcg tggaagagga
    26281 actgcggcgg aatcttgccg tcacggcggc cggagcggtg acggaggcgc atgtgaccgc
    26341 actgacgttc acgccacagg agacgaaacg tgtccggctg gtgatccgca gcactcaagg
    26401 gggcgaggcg ggggtggatg aactggaagt gttcggtcct gtggggacaa ataatcttgc
    26461 actcgcggcg cggggcgtgc gggcgagcgc ctcgtccgtc atcaaagggt atgcgatcca
    26521 cgcgaccgcg catctgaatg acggactgta cggcaatgac cacagttgga tcgcgggcgg
    26581 taccggtgaa gagtgggtcg agatcgagtt gcccgcaccg gcatccgtgg cgcgcgtcgc
    26641 attctcgcgt gaccgcacgg gccggtacac cgaccgccag ccgcgcgagg tggaggtgcg
    26701 gctttcaaaa gagggtcggg cgtgggaaac ggtctgcaaa cataccggtt ccgcgcaggt
    26761 actgccgacg ttgatgttgc ccgcatccgc cttgagcgaa ccgacatggg acggggtcgt
    26821 gacttacgcc tttttgtgcg agcgcgaaac gtggggtacg ctggatggca aggatgagtt
    26881 gtcgccattg cgcaacgacc gtccggccgt gcccggcgcg gaaccgtatt ggggcaaact
    26941 ggcgcgcctg ccgcatgtcg cgcggaatct gacgcagttt ggcggtctca tcacgcgcct
    27001 cgctgaacaa ggcctggatg tcacggccga acgcaaagag gcagagacgc tccgtgcccg
    27061 cctgcaggtt gaaggcgaaa cggatgcgct ctatctggcc gtccgccacg ccaagcgccg
    27121 cctctttttc cgcgatccgc ggctggcgcc ggttgagcgc gtgctctttg ccaagcgcca
    27181 tccgttgcag ccgtcgcaca actacagcga gcatatggat gggaagttca cgtcgggcgg
    27241 cggtatctgc gtgctccatg tgccgcgcga tgcggcggga cgcctcgatc ccgcgcgggc
    27301 cgaggtggag acgctcttcg acgggaacga cggcatcgtg aggcatccgg tatcggattt
    27361 cgacgcgggg accgtcttct tcgtgtaccg gcccgacaag cccgaggtca aagggtggca
    27421 ttcgtactgg cacctctggt ccgtcaacgc cgacggcggc ggtgcgcgca agctcacgga
    27481 gggacccttt catgacttcg atccggttgc gttgccggac ggcgatctgg ggttcatcac
    27541 cacccgatgc gccgcgcgct ttctttgctg gcagccgcaa gcctacgtgc tctaccgtat
    27601 gaaccggcat ggcggggaaa tgcaacggct ctcgttcgcg aatctaagcg agtgggatcc
    27661 cgtgatgatg cgtgacgggc ggatcctctg gacccggtcc gaatatcagg acaaaggcgc
    27721 cgatttcggg cacacgctct ggtcgatccg tccggacggc acccatccgg agttggtttt
    27781 cggcaacaac acgccatatg ggtacggtca cgcgcgcgag gtgccgggca gcgcggagat
    27841 cgtctgcaca ctgatttcac acggcgacca tcagggcccg atcgcgttgc tggatcgctc
    27901 caaggggctg tatgaagttg ccgcgatcac aagcatcacg ccggacacgc ggccgcaata
    27961 ccagatgggc cgttcgcatc atcagacttt ccgtttcccg gagccggtct cacgcgacca
    28021 ttttctagtc agccacagtc cgggtcggcg ctcgcactgg gggctctacc tcatcgaccg
    28081 ctacggcaac cgcgagttgc tctacctcga cccgttgatc agcagcaaaa agccctccct
    28141 gttccgtccg cgtccccggc caccggtgat gccccccatg cgggacgcgg agctggccgc
    28201 gcaggagctc gggcagttca cggtgcagga tgtgtacgag gggctcggac cgacggttcc
    28261 gcgaggccgg gttaaatata tccaggtctc gcaggagatg cccgcgatgc tggagcaatt
    28321 gagctgcggg cagtaccgca acacgcacgg tccgttcatg gatttctacg cgacgccggt
    28381 gcacaaggtc aagggtccgt cacacgagat cgtgacgcgc acacgcaacg cactgggggc
    28441 gcacgctttc cgtcagggca gtgcggtaac acgggaggac ggcacattcc ttgtgaccga
    28501 gcgcggcggc tggccgagtt ttgtggccaa gagcgtgctg ggcacggttc cggtgcaggc
    28561 ggacggctcc gccaacttca ccgcgccggc cggcaaggtt ctttacttcc atctgttgga
    28621 cgagcacttc aacgagatcc agcgcatgcg cagtgtgctg caactccagc cgggcgagcg
    28681 gcggagttgt gtcggttgcc acgaggaccg gttgcagttg ccggccatgc ggcagacgac
    28741 cgaggcactg gcgcaaccgg tacgggcact ggatccgtcg gcgtggggtg ggggaccgtt
    28801 cgattatgcg gctgtggtgc agccggtgtt ggatcgacgc tgcgtatcct gccacgacgg
    28861 tatgaaaaaa ggacgccccg atctgcgcgg gacaccggac gcggattttg tcccgacttc
    28921 ttatcgcacg ctgatctcgg gcggatgggt tcattatttc gactggatct ggggtgcgcg
    28981 gcacttcaag gccgagccat tgaccttcgg caccctacgc agccgtctgt tcgacgcgct
    29041 gaaaaaagaa cagcaccgag aggtcaggct cagcgcggag gaacggcgca cgctgacggc
    29101 gtggatcgac ctcaactgcc cgctgtggcc tgactaccgc taccgtcccg aacgttccgg
    29161 cgcaccgctc caggcttccg tccggtaggg cacgaggttt tgagtgagag gatgaaaagg
    29221 gagagcatgg agatacggtc cgtcagaaga agagatcgcg ctcgccattg gcgatccggt
    29281 cataatgctc tttgacggtc gggtagaatt tcggcagggt ggtctcgatc tcggccagtt
    29341 cccgccgcag caacggctcg cacaacatgc gtgtggcggc actggcgaaa tcgtcgagcc
    29401 actcacggaa ggtgagcacc gcgttgatct tgcagaactt tccttccttg ccggtcttga
    29461 gcgcgtccat gatgcagccg ccggtgcgtc cgcaacgata cccggcggtg cagaaagagg
    29521 tgatgatgcc ttcgcgagcc aagtgcgcga tcagttcttc gagggtccgt gtgtccgaga
    29581 gcacaaattg ctggcggtgc ttttcctggt gggtctcgct ggcgaagtcg ctgtaacctt
    29641 tgatcgcgat gttgctgctc gcgtccatct gggtgatccc gcagcgcacg aacgcatcgc
    29701 gcgattcggg ccgttcgcgg caggtcacga tcatgccggt gtacggcacg gagagacgcg
    29761 tgatgatgag gatacggcgg agggtggcgt catcgattcg atagggcgag gtggtcggca
    29821 gatccgttcc gctggcgggt tccagccgcg ggaaggagat cgtatgcggg ccgataccga
    29881 actggcgttc aaggtcgcgc gcatggcaga gtgaggcgag cagttcgaac ttccagtcat
    29941 agagcccgta cagcacaccc atgcccacat catccacccc cgcctccaga gcgcggtgga
    30001 aacaggtggt gcgccagtcg taatcaccct tcaccgtgtc ggcggggtgc aggcgcgcgt
    30061 atgtcgcgcg gtggtacgtc tcctgaaaga cctggaaagt gccgatgccg acttgcttga
    30121 gcacacgcag gtcctcgatg ggcagcggtg ccgcattgac attcacgcgc cggatgccca
    30181 ccggcgtgcc ttggggcgac ttgacttggt gtgcgtaaac ggtgcggatg gtttcggcga
    30241 aatactcggt cgaggtgtcc gggtgttcgc cgtagacggc gatcaggcgt ttgtggccga
    30301 tcttgcctgc cagcacgtcg acttcctgcc gaacctcgtc catggtcagc atgcggcgct
    30361 tctgatcctc gttaccgcaa cggaatccgc agtaggcgca acggttgacg cagaggttgc
    30421 tcatatagag cggggcgaac atcacgatgc ggttgtcgta gaccgcgtgt ttgatcgcca
    30481 aagccgtggt ctccatctcg tcaagcaggg cgggatcggt gacctgaatc agggatgcgg
    30541 tctcttcggg cagcagggtt tcgatggcgc gtgatttcgc gaggatttca cggacgcgga
    30601 ccgggtcgtt cgcgggcgcg gctgaggcgg cggcaagaag ccgctccagt tccgcctcgt
    30661 caaggtagtg ccggccgccc ggcagatagc ggtcgatctg ttcccggatg atgaaacgtt
    30721 tgcggtagtc ggccgcattg gcgaaacgac gcaacggagc ggatgtcaag gtctgtgcgg
    30781 tcatgaggcc tcctcggttt gatttctgcg gggcagggga ggcaccggac cgggttcgaa
    30841 aagcggccgc gccgacaggc cgtcgaccgg atatgcggtt cctcgggtca gagccaggct
    30901 cctccacctc agaaaacgca tctacggtac acgttcgagc ggtcaaacgc aagattcaaa
    30961 cccaaataaa ttgaacgtcc gcaagtcaaa aaaagaggtg tctcaaatcc gtgttgttaa
    31021 tgtgggtggt gtggtgaaac aatcgatctc gatagcgata ccgatagcga tcccgatttg
    31081 gatggaacct tgaaacacag aaaccctgaa accctgaaac cctgaaaccc agaaaccaga
    31141 aaccagaaac cttgaaacca gaaacccgag agagtgttcc gtggttaaat gatcgattcc
    31201 gatagcgata ccgaccccga taccgatttg gatggaacct tgaaaccctg aaacccaaga
    31261 aagtgttccg tggttaaatg atcgattccg atagcgatac cgaccccgat accgat
//
LOCUS       DAOSRB010000008        31103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1609_length_31103_cov_6.668996, whole genome shotgun sequence.
ACCESSION   DAOSRB010000008 DAOSRB010000000
VERSION     DAOSRB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 31103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31103
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1609_length_31103_cov_6.668996"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1146)
                     /locus_tag="PLZ74_00935"
     CDS             complement(118..1146)
                     /locus_tag="PLZ74_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96920.1"
                     /translation="MCTSMLLAAAVSVFTVENPKGYNSWPMIGETGGKLVCAYSKGLK
                     HAIGEGERGAYVRTSSDGGRTWGEEVCLANAPDLGEVPIGKGKDATGAALFWNRCCGS
                     TSRHDLYRTTDGKAFERLASLKLDPHPTQITDIFEVDGNLMSFWFRCSYGDKKGLNAW
                     GTVISKDGGRTWGQVLVESGLTPTELPTEQAGFYAGNGRVFAIARTEGGAGQFQLTSL
                     DSGKTWKKTRTNIKDVYCSTPSLIYDAKTDTIYNYYYQRGAGKLKRRVAFLADVFGHP
                     TRWPEPDVLVTDTPVPPAFDCGNANAVRLGDKHVISYYTGKYPDTGVFVCVVDVGKTE
                     VVARPLGF"
     gene            complement(1246..3180)
                     /locus_tag="PLZ74_00940"
     CDS             complement(1246..3180)
                     /locus_tag="PLZ74_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOR96921.1"
                     /translation="MSRFFCVTMMLIAGTAFTKQPPNNADMKVSSSGLWAIFGSFGKI
                     CGDAAVVPDGYRLEESGCRIETTVETDKGVSLRKTVLKNVSDKTITAYCLMDRFPIED
                     GEWEVYTQVNYWENESQGQWQPLHTAVEVRNPNLRSSHGAAPMLAVWNRQAGRGRVFH
                     LLSDSAWELHAVCSNPGGNRTSFAVEAGMDSRQLAYSLAPGEHVALPEVLSYEFENRL
                     DLDCHKLHAWWNRHHPGRPPAAIYNTWLCRFDKLDADFLLKQVARAKDLGMEYFVIDA
                     GWFGPAAGWASVRGEWAERPDGHLKGRMKEISAAARKADLKFGFWVEAESVTGETRVA
                     REHPEWLLPANRSFLDFTNPDARRYITETVCALVKEYDASFLKFDFNQNLDHDPTGRA
                     FAAYNAAYRAFVRDMRRRNPGIYIEGCASGGYMMDLGWARDFDGFWLSDNQSIGHGLR
                     IVRETMLRFPPRQIERWLTVRSLTDIQPDYGGGDERLVSTEDAVWRNLRSYPLESAAA
                     FTAGGPLCFSCDLTALSENHFAYFQKLVAAHRQDAAFWARAVGRVLCAESSVQAVQYS
                     DEALTDVRIFVTADSSTQRTVTVTPVVDPSARYVRDDGHESTGAALRASGVSVALDRL
                     AAKEIRLRKCMGSSVTSVKK"
     gene            complement(3966..5270)
                     /locus_tag="PLZ74_00945"
     CDS             complement(3966..5270)
                     /locus_tag="PLZ74_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOR96922.1"
                     /translation="MDRSAKIFLMRAYKDRVVDSLLRRKLEGTGAVLIEGPKWCGKTT
                     TAEQVAQSVIYMAEPQKRDDNIAMARMDPGRILSGARPRLIDEWQIAPVLWDAVRHDV
                     DHAGDLGRFILTGSSVPANEDEIKHSGTGRFAWLKMRPMSLWESEESSGEISFRELFS
                     GANVQTASAKPLELEEIARIVCRGGWPQAVSLSGNAALDQAYNYVDAITKKDISRVDG
                     TTRDEDRARRLMRSYARLQGTQASASVIKEYLSTNEMTSIDNGTIYAYLNALRRLFVV
                     EDMPAWNPNLRAKDSIRISDARYFVDPSVAAAALEIGPEDLMNDLTTFGLLFETMAVR
                     DLRVYADALGGTVRHYRDRHGLECDAVVHLRNGAYGLIEIKLGGDKPLQAGTETLTKL
                     ARLIDSKKVRKPDFQMLLTAVGSFAYRRDDGILVCPLSALRP"
     gene            5650..6300
                     /locus_tag="PLZ74_00950"
     CDS             5650..6300
                     /locus_tag="PLZ74_00950"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOR96923.1"
                     /translation="MSNLSPTPGSSRIVKHLRTRLVSGLLVLIPLAITLFVLNIFLNS
                     LTAFARPLLKPLLGVLPESVLTLIALTLTVLLIYLTGLVTTHIVGRRLILFGERLLLR
                     LPVVKTIYAASKQVVETFSSHQTTSFQAVVIVSFPRRESLAIGFITGTLFAPDNRKLY
                     RVFIPTTPNPTSGFLLLMSPEDVQFTDIPIEEGIKMIVSGGMLAPEKYAVVPDNCP"
     gene            6359..7000
                     /locus_tag="PLZ74_00955"
     CDS             6359..7000
                     /locus_tag="PLZ74_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96924.1"
                     /translation="MAKPRREVEITLHSIRVPVTGTGLDTDTSHLLTATLIWPRLGVA
                     GKSASQPCALQHGESSFETVHWGRRILFKEAVEGRFALGLVLTEALDDEEMEKFLRFW
                     TGAFLAVGADLAGAAAPAPAGKLAAAPLDYAAKAIAKYPGALPLVEGLVEINAAELPP
                     GGRERLLTVRLTAARTLARLSRRTVNSRPRVSRSLLLEKGADNGEVTLAIRSL"
     gene            complement(7002..9530)
                     /gene="secD"
                     /locus_tag="PLZ74_00960"
     CDS             complement(7002..9530)
                     /gene="secD"
                     /locus_tag="PLZ74_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOR96925.1"
                     /translation="MSRNDLWKWLILAFLTVLSAYVVIPPKEKIRLGLDLSGGTSFTV
                     AIDEAKLRANVRAAADTELTDVEVENQVNAVLRDADARAIEVIRNRIDGLGVNEPVIQ
                     GGKNHRIVVQLPGASAEQRDAAEKSIQSAAFLEFKLVHKQNEQLVDKVLASGRLPEGY
                     VSSDDGRGYKRAPNYTELVKDPEYMHRLSMFEVPDPRYAFMLEREQTANGQVIYRPIY
                     VLRKAEMTGDALSSASVEIEPMTGRVHVSLVFNAKGGADFARLTKAYAPRGSRNKDSD
                     VGRRLAIVLDDTLYSAPVLKTEIPNGRAVIEGNFSWSEAAVLRNILNAGSLPAPMKIL
                     EKRSVESTLGADAIRSGIQASILATVLVALFMLVYYAYCGLIANVALLLDLLLLPAGL
                     IMASSILGVFVKDTAIAKRVLDLPVLTMPGIAGIVLSLGMAVDANVLIFERMREEFAL
                     GKSARAAVAAGYDRAFLAIFDSNITTLLTAVILFIFGAGPIRGFAITLTAGIIISMFT
                     ALVVTRLIFNATVPENRTRPYKMLQFMKNSNVDFLRWGKPTGYTSLAIIVVTLAIFFA
                     RGLKDPASVMAVDFTGGVSMTFTYEQKADIGTVRKAVTDAVKNDATIQYQSTLDGSGN
                     LLLVKTSVTQIGEESASKVIQKALAKELPESRFTLAGEEDVGSEIGGDLKLSAAKAIL
                     FSLIGILIYVSLRFEFGFALGGVVALAHDAFITLGLYSLFGRQVSLTVVAALLTIVGY
                     SINDTIVIFDRIREDMRKDVKMPFPELCNRAINQTLSRTILTSLSTLIATVTLFIFGG
                     GSINDFALAMMIGLIAGTYSTIFIATPVMLAWYRGRRPAFVTAK"
     gene            complement(9633..9968)
                     /gene="yajC"
                     /locus_tag="PLZ74_00965"
     CDS             complement(9633..9968)
                     /gene="yajC"
                     /locus_tag="PLZ74_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOR96926.1"
                     /translation="MTTGWFWAQAAQQGTGGVGGMLIPMVLVFGLFYFMMIRPQQRKE
                     KERLRMIAELRAGERVMFAGGLIGTISEARETTFVVEIAPKVMVEVARGAVTRVLKEG
                     DVATLEEGR"
     gene            complement(10032..11198)
                     /gene="tgt"
                     /locus_tag="PLZ74_00970"
     CDS             complement(10032..11198)
                     /gene="tgt"
                     /locus_tag="PLZ74_00970"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOR96927.1"
                     /translation="MRQKPGVFEILKEDRHTGARLGRLWTAHGQIDTPVFMPVGTQAT
                     VKALEPRDLTDLGASVILGNTYHLLLRPGMDVVSACGGLHRFMAWDGPILTDSGGFQV
                     FSLNSLRKIRAHGVEFRSHIDGASFFLGPSEAMAVQRTLGSDIAMCFDECIPYPCDEQ
                     YACQSVAKTLSWAALCLKQERAPGQLVFGIVQGGEYTALRERCARELAAMGFDGYAVG
                     GVSVGEPEDVMLRGVEDGVRGLPRERPRYVMGLGDLYQMSESVARGADMFDCVIPTRV
                     ARHGTAYTRTGQYPVKAGLHKADTRPVEEGCDCYCCRTFSRAYVRHLLNVGEILGVRL
                     LTIHNMHRYLGFMREMREAIANDAFGEWRRALAAVLRPDGRKPVRMGARAPQAG"
     gene            complement(11233..12687)
                     /locus_tag="PLZ74_00975"
     CDS             complement(11233..12687)
                     /locus_tag="PLZ74_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOR96928.1"
                     /translation="MSDTTDVKKTVWERVTTFLRHDLWEMRSSELPWLYRSGVYLLRM
                     GHLVVDGFRRNACSLHAASLTFFTLMGLVPILVLALALARTFGGADLAKEQLDRHLNA
                     WMSQMEAAVDARTAEIAEGDAEKNQVHEEVTKAFSTQVREIADQLFDQVNSISFGTLG
                     GIGAVMLIWSVVGMLGKVEDSFNRIWGVERSRVLVRKLADYLFVIIILPFLIMVVSSM
                     PIAKMITDAMDKTVGGAVSDGVRMVVNSSLLKSGITLTVGTLTFAFLLGFMPNVRVQT
                     GAALVGGLVTLVLFGGWLKLCAMLQVGIAKYSALYGGFAVLPILLMWVYTSWQIILLG
                     SEIAFAVQNRDTYVLEQNASRASLRSRLLLALTLCAEAAHCAKDPAGGPFAAEAFARR
                     RGISSRFVKDILEDLARHGILAEVAGRPGDYLLARCGSSLTAAEIAKALLDEGEPPEA
                     LGLTALSAPLLAFNQRVDERIEEGFGQPVAALNG"
     gene            complement(12684..13904)
                     /locus_tag="PLZ74_00980"
     CDS             complement(12684..13904)
                     /locus_tag="PLZ74_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR96929.1"
                     /translation="MSERGREKPRRTGFVRTAVRVALGVAAAVCALQGVGPAWLSVVF
                     PSLSPFTALLAAAAGAGSVLLWGAAAVAAVCLFLPRFFCRWICPAGTCFEGVGFRARR
                     RAWVGRVPRIGVGLVLVGTGAAVLGYPLFGWLDPLVIFQAFFAFFRPGDVAWESMAAA
                     GLPLLILMSLLTPGLWCGRLCPLGATQDLLRAAVTWQRADGKDEASRTVRGTGAGRRI
                     FLGLGAGAVYRLLLPPERAAAGAGVIRPPASGTSARFTRLCARCGNCVRACPSHILHG
                     GGVGAGLAGALAPEVRFGENYCPTTCTVCGQVCPTGAIPRFTRQNKFRNPMGCAQVNP
                     AICLLTEGRECGSCVAACPDAALDFAWDPVDLVSRVTVDAKVCTGCGYCEYVCPVADK
                     AIRIRAPGRKEGAG"
     gene            complement(13964..15139)
                     /locus_tag="PLZ74_00985"
     CDS             complement(13964..15139)
                     /locus_tag="PLZ74_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOR96930.1"
                     /translation="MKITGIKTFVCNAYRTNWVFVKVLTDEPGLWGVGEATLEYKEQT
                     IVSACMDLERDLIGKDPRDIEAIWHSNYRDAYWRGGPVLMSAISAIDIALWDILGKSL
                     NAPVWRLLGGCMRDKVPCYANGWFAGAKEPEEFAAKAVEAIETAGWKGLKWDPFGTAY
                     RQLEPEAFRKAMRCVEMLYEAVNGRAEILIEGHGRFDLPTALRIANALEAFDILWFEE
                     PIIPDTIEALADLRSRVKVPLSFGERLYGTWQFRALLEARGADFVQPDVSHAGGITEL
                     RKIAAIAETHHIPFCPHNPSGPVANAATLQLAASQPGFYLLETMASDVPYRREICDEA
                     VTLRDGYMHIPLDRPGIGVEIDEAAIAKYPYQPISLRHYIGTLTEIRPQNATAYFTK"
     gene            complement(15151..16410)
                     /locus_tag="PLZ74_00990"
     CDS             complement(15151..16410)
                     /locus_tag="PLZ74_00990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR96931.1"
                     /translation="MKPSKSYKWELLALLSFAFFFHQADRALFSVVLIPIQTELGLTD
                     GQMGEIGSWMFATLAVMVPIAGFMGDRLSRKWIITCSLIFWSCATAMTGMATGVLGLI
                     LFRSVATGGGESFYAPSAYALLASYHKETRSVALSVHQAALYVGLMCSGYLAGLVEAA
                     FGWRSVFYLFGGAGVLLGLVFIFRLKDAPAEPRPDKPAAVNKPSVWESVRVLVKTPSA
                     LLATAGFCAVVFVNNAYVFWAPTFVMEKFKSSATPMTLTQAAGGAMLYHHLAAFGAIM
                     AGGVLTDWAVSRYPRFRLLLQGAALLLGAPMIYWIGVANGITATWIAMAGYGIFRGLF
                     EVNTHASLFDVVPAKFRSTAVGLMTMTAFFLGTVPPKLIGKFCDLHGKVAGIQQGFTV
                     LAFAYVLGAVAIFCSMLFTFNRDRVLE"
     gene            complement(16407..17183)
                     /locus_tag="PLZ74_00995"
     CDS             complement(16407..17183)
                     /locus_tag="PLZ74_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR96932.1"
                     /translation="MRFKGKVVLVTGTSRNTGVGIAALFIREGAKVCVCSSSPESTAK
                     GAAELRAMGLTEFTETPADISDTEQVRAMFRVIREKFGRLDILVNNACNQGIGPAFEE
                     MEPAYFLSVVKTNLLGTFQVSQEAVKMMLAQESRGVIVNLGSNVSMRAIRNRTAYVAS
                     KGGLDALTRSMAVDLGPKGIRVNMVAPGYIYTDRWDKLNEATMKRRRLNCPIGMEATA
                     EDIAQAVAFMASDAARNICGERLVVDAGCSAQHMPVDVDI"
     gene            complement(17225..19318)
                     /locus_tag="PLZ74_01000"
     CDS             complement(17225..19318)
                     /locus_tag="PLZ74_01000"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOR96933.1"
                     /translation="MRRLSWLGCVWLAMGCLAADVVLFDAARTPLSAVASHSGGTFTL
                     TDGLLAIETKGNLGYPGVRIAGEWDLSGCNSVTFELEHCDGKGELPLTVRFDNPGADP
                     GKSTGVFVDRIKMPRKGAHACTVTLPPTLPSGRAISSKLTGMRTTPFQTTGVVADLDT
                     ARVVGVAVYMRLNKLDWKWGVRRIVAHTGPVADVPSWMKLTEKEFFPFIDVYGQFKHK
                     TWPGKIHSDEELKTAARREEADLAAHPGPKGWSRFGGWADGPKRAATGHFRVEKIDGK
                     WWMVDPDGYLYWSHGPVRVTPSTAITPLDNREFYFEGLPPREGSPFSLFYTTRDELLY
                     PYYVKRNIQRTYDFSAANLLRKYGGTWFETFADLAHRRLRSWGMNTIANSSDKKICLM
                     DRTPYTDRFELKSKPIAGSHDGWWPFRDPFDPSFRADVRRLMNEHKAEMQDPWCFGFF
                     VDNELSWGGETDLAAWTLESPADQAAKRVFRERMRKAYGTIDKLNAAWGTAYADWEAF
                     LTTRTRPGKSAEPDLKAFTLEIADAYFRNIREEFKAAAPNKLYMGCRFAGRGPEFAVR
                     VAARHCDLISYNIYARHLNDFKLPEGIDKPVIIGEFHFGALDRGLFHPGLIKLENQEE
                     RGRVYVQYVTSALKHPHVVGVHWHQFGDQATTGRFDGENFQVGFVDCCNTPYPETIAG
                     IREVGYRLYEIRSGK"
     gene            complement(19583..21997)
                     /locus_tag="PLZ74_01005"
     CDS             complement(19583..21997)
                     /locus_tag="PLZ74_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96934.1"
                     /translation="MMKKMMLSIAGVMSALAVSAVNISADIAKAAYYTNATVITLTNS
                     QDIMISGSGTIDVILIGGGGGGGANRVDLSQGGGGGGGGGVILRRDVAVSAGVYPIEI
                     GSGGTTGVIGNATGTADNGGNTTAFGITAQGGGGGANYSNNGQARQGRAGASGGGSTA
                     YNATAVGGASIAAQGEYGNDGGGSNHLYGPGGGGGAGAPGVTSGGSAPGGGGDGIELA
                     VYGDYGVYYGGGGAGYRQGTFASGGKGGGGSRYEKGVDGLGGGGSGGYDGGCGAVVIK
                     FTPTPVSDTKDFLLTGGDEKILLTNGTVHVFRQSGKLTVAGSGCVEVLMVGGGGGGGN
                     HTYAGGGGGAGGLVHISSARITAGEYDIIVGAGGAVGANGGDSSFLGALAHGGGAGGY
                     NSTGSSGGSGGGGSTISANKVPGGSAVFAIDGNIGDVGGASGANIYAPGGGGGAGGPG
                     EDGVDSTSGTANIPGDGGDGLAFDITGDNVWYAGGGGGGEPNRGAKGGKGGGGDSHVA
                     GVDGLGGGGGGYAAGGDGIVIVRYAKKSFYRDYIENATGGTVTRSEGCNIHTFTEDGT
                     FSLPEAGVVEFLMVGGGGGGGVNLDTDPAQGGAGGGAGGLIYRDVVVLNAGTHQVKIG
                     AGGNVGENGGNTEFVRFVAYGGGHGGGRSGNARKPASGGSGGGGIWISGGAYVPGQAI
                     HGEQGNLGNDGGECSHVYAAAGGGGAGAVGGNSNGTSPGIGGDGLAFDISGTLTYYAG
                     GGSGYRKGSGYPAMSQGGLGGGGPGGQNGVDGLGGGGGGNAAGGSGVVIIRYLLPKKG
                     FVVICK"
     gene            22333..23238
                     /locus_tag="PLZ74_01010"
     CDS             22333..23238
                     /locus_tag="PLZ74_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96935.1"
                     /translation="MFYLFLLFHFVFLLFWLRFWSAPEREFYFNPFVSGTINLTDRVI
                     LFLRPVLAIPEAMTALVILIVVVLLQTLLAARLQFPLGVTLGTTFHFTPPAVSDNPLL
                     PLPFTLLQNALFLVRLWTVYLLVRLITPPFRVTRATEALAFFARPFSRIPLLFQPFLL
                     LALHGLIALALTRTTELRILTRLTETPAATDVSPFLTGPLLLQLLKTGWLAVLSFSDG
                     LIFMTRSLFVLIIGSLGAAIIQSRGLAVLCSEAVDLLLGRFARRCTGAMGFDFTPLIY
                     FFVVDFLYNGICRGLYMLIHSPLFN"
     gene            23246..23536
                     /locus_tag="PLZ74_01015"
     CDS             23246..23536
                     /locus_tag="PLZ74_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HOR96936.1"
                     /translation="MSWVVETRDGCVVTVKATPRANRSEIAGVDADWLRVRLQAPPVD
                     GKANAALIAFLAKVAGVPKRSVVLLSGETSRLKRVALCGVTAAAARQAFAKQ"
     gene            complement(23561..24067)
                     /locus_tag="PLZ74_01020"
     CDS             complement(23561..24067)
                     /locus_tag="PLZ74_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOR96937.1"
                     /translation="MDGSTARHGNYRKILFCTDFSANADFAFHFAVDAALRNTGSTLY
                     LLHVIPEPDAQFWKGYIYEVGDMDAKARADIDRMIDTVYRPRVPSDVTFQIAMRIGDA
                     ARMILDFAASQDVDLIVLGRQGRGAITQWLLGNVTGKVVRRSTCPVLVVPQAFKTRQS
                     EPAAADEQ"
     gene            complement(24089..25390)
                     /locus_tag="PLZ74_01025"
     CDS             complement(24089..25390)
                     /locus_tag="PLZ74_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOR96938.1"
                     /translation="MTPVMFGVVGCVALFILLLCSMPVAIVMGLVGVVGYAAVVSGKA
                     ALAMLSADLYDVFSSYNLTVIPLFIFMGQVAFHTGISGRLFTAANSWLSGLKGGLAMA
                     TVGACAAFGAICGSGPATAATMAAVALPEMRKHKYDDALASGTVASAGGLGMLIPPSV
                     VFIVYGILTEQSIGKLFIAGILPGIVVALLFCLTIHVLCLANPALAPKAVRTPWRVRF
                     RSLSGVTETMILFVLVMGGMFAGWFTPTEGAAIGAAGSVAIAVCGGHGSWALFRQAAQ
                     ETIRTSCMVLVIVAGATMFGHFLAVTGLPTQFAAWLAALPLPSWVVMLLILFFYLLAG
                     CFVDSLALILLTIPIFYPVVMGLGFDPIWFGVMIVLVTQMGVITPPVGVNVYVVSGIA
                     RDIPLQTVFRGAIPFLFALIVACGILMLFPGLATWLPGVVR"
     gene            complement(25387..25905)
                     /locus_tag="PLZ74_01030"
     CDS             complement(25387..25905)
                     /locus_tag="PLZ74_01030"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOR96939.1"
                     /translation="MSPAQKPVRKPLGPFWQSVVGLCRFMTGIACCGLAVMTALVCVD
                     VVLRLAGHPVKGVYDLVRIVAAVTVACSLPITTAKKGHVAIEYFFLKLNRVGRLVVDS
                     VMRLLMIAAFLLTATECVGYGLRFLRNRQVSDTIEVPIFWVPWVMAVAFAVTALVVVF
                     HLVYPGREMVKS"
     gene            complement(25902..26954)
                     /locus_tag="PLZ74_01035"
     CDS             complement(25902..26954)
                     /locus_tag="PLZ74_01035"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOR96940.1"
                     /translation="MSRSQYGAVLFLLFAGWIVAAGCGKQETTAADGSRPVKLTYSVF
                     FPPTHKQAKLAEAWAEEIGRRTQGKVRITVFCGGSLTKADQCYQGVVDGISDIGMSAF
                     AYTRGRFPLLEGLDLPVGYADGVSATRIVNELCHAFLPKEIRDVKLLYVHAHGPGILA
                     SKRPMRTLADLKGAKVRATGFCAKVVESLGGSPVSMPQGDTYEALQKGVVDATFCPVE
                     TLKGWKQGEVIRNITDSRCIGYTTSFFVVINPVRWASLPDEVKQIMEDVSKEWVERHA
                     QAWNEADDEGLAFARSLDREVVPLDPSEQVLWRKQVEPLLREYAERAEAKGLPGKDFL
                     NRLTTAVREAQEGSSR"
     gene            complement(27107..28372)
                     /gene="icd"
                     /locus_tag="PLZ74_01040"
     CDS             complement(27107..28372)
                     /gene="icd"
                     /locus_tag="PLZ74_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOR96941.1"
                     /translation="MAYQHIQIPSFGEPITRGDDGRLHVPDCPVIPFIEGDGTGPDIT
                     RAARIAWDAAVQTAYGEKRKIAWMEIYAGEKANAVYGPGTWLPDETLEACKAYLVSIK
                     GPLTTPVGGGIRSLNVALRQELDLYVCLRPVRWFQGVPSPVKRPELTDMVIFRENTED
                     IYAGIEWLAESDEARKVIAFLQNEMGVKKIRFPGSSSIGIKPVSIEGTRRLVKAALDY
                     AIDNDRSSVTLVHKGNIMKFTEGGFRDWGYQLAREAYGAALIGDGPWCRFKNPKTGRE
                     ITVKDCIADAFLQQILTRPAEYDVIATLNLNGDYISDALAACVGGIGIAPGGNINYTT
                     GVAVFEATHGTAPKYANLDKVNPGSLILSGEMMLRYMGWTEAADKLISAMDRVIANKT
                     VTYDFARLMEGAVEVSCSAFGRALADAMQ"
     gene            28717..30015
                     /gene="larA"
                     /locus_tag="PLZ74_01045"
     CDS             28717..30015
                     /gene="larA"
                     /locus_tag="PLZ74_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOR96942.1"
                     /translation="MPTIRIPYHRGHLEATLPANVRLTLLEPTGTACTVSPSEAEQRQ
                     RVCAALDHPVGTPRLETLAAGKASAVLITSDHTRPVPSRITLPLLLERLRRGNPGIAI
                     QILVATGCHRPTSDAELREKFGAELVARETFVMHDCTDTANLRMLDQLPSGGALWLNR
                     LALETELLVAEGFIEPHFFAGFSGGRKSVLPGIAGRETVLANHCAAFIADPHARAGQL
                     ERNPIHRDMLFAARQARLAFILNVTLHPDKTIRTAVAGHAEAAHEQGCRDMRRAVSVP
                     RAEAEIVITGNGGYPLDQNLYQAVKGMTAAEACCVPGGTIIMVAGCCDGSGGESFYRA
                     LAGARSPAELLAEVLRVPQALTPPDQWEYQILARILSRHRVILVTDLWDADAIRALHI
                     TPAASLDEALALAWHEHGTSARITVIPDGVSVIVEPKKSS"
     gene            complement(30443..>31103)
                     /locus_tag="PLZ74_01050"
     CDS             complement(30443..>31103)
                     /locus_tag="PLZ74_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96943.1"
                     /translation="QTTLEQPVIQMWTTNVPHLVQAANHIGETVAHVAETAARLATTA
                     EKLPAQVAAEREALVKTLRDEQEKTLTPLVEGVRETLLAGSGLATNATTMLATFDGVL
                     ERMGVDGSESDAPPAQVAAKEEGTPFRIQDYSEAAERLEASALRLTELLQTFDQTLGA
                     NSREALAAQLDPVVRRTRAETEALVDHLFRRALQLTGALLAAALAYRLIASRISRTPR
                     A"
BASE COUNT         6310 a        10584 c         8442 g         5767 t
ORIGIN      
        1 cctgaaaccc tgaaaccaga aacccctgaa accttgaaac cagaaaccca gaaacccaag
       61 agcgttgaag gttcaactgc tctttttagg tttaacctta tgtttcgcaa acgagcctca
      121 aaatccaagg ggacgtgcga caacctccgt tttaccgaca tccacgacac agacaaaaac
      181 cccggtgtcg ggatatttgc cggtgtaata ggaaatgacg tgcttgtcgc caaggcggac
      241 ggcattggcg ttaccgcagt caaacgcggg cggcaccgga gtgtccgtca cgaggacgtc
      301 cggctccggc cagcgtgtcg ggtggccgaa aacgtctgcg agaaaagcga cgcgacgctt
      361 gagctttccg gctccgcgct ggtagtagta gttatagatc gtgtccgtct tcgcatcgta
      421 tatgagcgag ggtgtcgaac agtagacgtc tttgatgttc gtgcgcgttt tcttccaggt
      481 ctttccgctg tctaaactcg tcagctggaa ctgtcccgcg cccccctcgg tacgggcgat
      541 cgcgaaaacg cgcccgttcc cagcgtaaaa gcctgcctgt tcggtcggca gttccgtcgg
      601 ggtgagcccg ctttcgacaa gaacctgtcc ccacgtccgg ccaccgtctt tcgagatgac
      661 cgttccccag gcattgagtc cttttttgtc gccgtagcta caacggaacc agaaggacat
      721 gaggttgcca tcaacctcga agatatccgt gatctgcgtc gggtgcggat cgagcttcag
      781 cgaggcgaga cgttcaaagg ctttcccgtc cgtcgtgcgg tagagatcat ggcgcgacgt
      841 gcttccgcag caacgattcc agaaaagtgc cgcccccgtc gcgtccttgc cctttccgat
      901 cggcacctcg ccgagatcgg gcgcattggc gaggcagact tcctcacccc acgtccgtcc
      961 gccgtcggac gaggtgcgca cgtaagcacc gcgctcgcct tcaccgatag cgtgcttgag
     1021 gcccttcgag taggcacaga cgagcttgcc gcccgtctcg ccgatcatcg gccatgagtt
     1081 gtaacccttt ggattctcca cggtgaatac ggatacggcc gccgccaaga gcattgaagt
     1141 gcacatcaaa aaaaccctcc ttggctaatc agttgttttc gaatagtgat tttactttac
     1201 ctctttcatg aagaactcgc tcgttgaata tccgtaaaac cacgattact tttttacgga
     1261 ggttacggat gaacccatac acttccgaag tcggatttct tttgcagcca gtctgtccaa
     1321 ggcaaccgac acgccggagg ccctcagcgc agcgccagtt gactcatgcc cgtcgtcgcg
     1381 gacatagcgt gccgatggat ccacaacagg cgttacggtg acggtccgct gagtcgaact
     1441 gtcggccgtg acgaatatcc gcacatccgt gagggcttcg tcactatact ggacagcttg
     1501 aaccgacgat tccgcgcaga gcacgcggcc gacagcccgc gcccagaatg ccgcatcctg
     1561 acgatgcgcg gcaacgagct tctggaaata agcgaagtgg ttttctgaaa gcgccgtaag
     1621 atcacaactg aagcaaagcg gacctcctgc cgtgaaagcc gcagcacttt ccagcggata
     1681 ggaccggaga ttccgccaaa cagcgtcctc tgtcgagacg agccgctcat cgccgccgcc
     1741 gtaatccggc tgaatgtccg ttagcgaccg taccgtcaac catcgttcga tctggcgcgg
     1801 cgggaagcgc agcatcgtct cacgtacaat gcgcaggccg tgaccaatgc tctggttatc
     1861 gctcagccag aacccgtcga agtcgcgcgc ccagccaaga tccatcatat accccccact
     1921 cgcacaaccc tcgatataga tgcccgggtt acggcggcgc atatcgcgca cgaaagcacg
     1981 ataggcagcg ttgtaggcag caaaggcgcg tcccgtgggg tcatggtcga gattctggtt
     2041 gaagtcaaac ttgaggaacg atgcgtcgta ctccttcacg agcgcgcaca ccgtctccgt
     2101 gatgtagcga cgggcgtcag gattcgtgaa atcgaggaac gagcggttcg cagggagcaa
     2161 ccattcggga tgttcacgtg cgacgcgggt ctcccccgtc acactctccg cctccaccca
     2221 gaacccgaac ttcagatccg ccttgcgcgc ggcagcggaa atctccttca tgcgcccctt
     2281 gaggtggccg tcgggacgct ccgcccactc gccgcgcaca ctagcccagc cagccgcagg
     2341 gccaaaccat ccggcatcga tcacaaagta ctccatgccg aggtctttgg cgcgggcgac
     2401 ctgcttcagc agaaagtccg cgtcgagttt gtcgaaccgg cagagccagg tgttgtagat
     2461 tgccgctggc ggccgccccg gatgatggcg gttccaccat gcgtggagct tgtggcaatc
     2521 gaggtcgagg cggttctcga attcgtacga gagcacctcc ggcaacgcca cgtgctcgcc
     2581 cggcgcgagc gaataggcga gctgacgcga atccatgccc gcctctacgg cgaaggacgt
     2641 ccggttgccg cccggattgg agcagacggc atgcagttcc caagctgaat ctgagagcag
     2701 atggaagaca cgtccgcgtc ccgcctgccg gttccacacc gccaacatcg gcgccgcacc
     2761 gtgggaagag cgcagattcg ggttacgcac ttcaaccgca gtgtgaaggg gttgccactg
     2821 cccctgactc tcattctccc agtagttgac ctgcgtatag acctcccact cgccatcctc
     2881 gatcgggaag cgatccatca gacaatatgc ggtgatggtt ttgtccgaaa cgtttttgag
     2941 cacggtcttc cgcagactca cgcccttatc cgtctcgacc gtcgtttcga tccggcagcc
     3001 gctctcctca agacggtacc cgtccggcac aaccgccgca tcgccacaaa tcttgccgaa
     3061 actaccgaaa atcgcccaga gtccactgga tgagaccttc atatcggcat tgttcggcgg
     3121 ttgcttcgta aatgccgtgc cagcaatcaa catcatcgtc acacaaaaaa accgactcat
     3181 ttcatgatcc cttttataaa acgtaatcta caagctcccc acacggaagt caacggcttg
     3241 tatcctcacc caaattttcc ccgctccccc cctcgcactt gaattgcatc aaccctcacc
     3301 cccctgtcac gaaccgatta cacgttcaac gctcaagtaa gtgcgcaaaa tttggagtgc
     3361 ggtgacaaga gccgcgctgt gcgcggagac acacaccaag tcctctcgcc agacttgcgg
     3421 tcacgccgct acaccgtaaa aacctttgcg tctccgcgtc tctgcgttaa aaaactttca
     3481 actctcaacg cccaactttt ttgcgggcga ggacgcccgc gctcccgatt gcgcatacac
     3541 aaaccacgtt ctcaactcgc atgccccctg tcaccaaccg attacatgac caccaataaa
     3601 cctgttgcac gcgcgtccct gagtggtgca taatgttatg cataaatgaa tgcgcatgat
     3661 gttctccttt ggtgatgggg aacgtcccgc cggggacggc tccagggtac ggattcccgt
     3721 tcgtccggca tgcgttctga aatgatctcc ttgcctataa attgacagga tgacaggatt
     3781 ttcaggattg aacaacatca tgttaatcat ggatttctcc ccgccgaagc gacgctgccg
     3841 gaaatcatgt cctaaactta tccagagttt tctcgcggcg ccggcgcgcg gcgggctaaa
     3901 tcctaattcc taaatcctcc tttttttgat agagccgtcc aaatacctgt cccttcgttg
     3961 gcggattaag gacggagagc cgacagcgga cagacaagaa tgccgtcatc acgacgataa
     4021 gcaaaactgc ccacggcggt caggagcatt tgaaaatccg gttttcgaac ctttttggaa
     4081 tcaatcaggc gcgcgagctt tgtcagcgtt tccgtccctg cctgaagcgg cttgtcgcct
     4141 ccgagtttga tttcaatcag accgtaagca ccattgcgta aatgcacgac ggcatcgcat
     4201 tccagaccat gacggtcgcg ataatggcgc accgtcccgc caagggcgtc ggcatagacc
     4261 ctcaagtcgc gaacggccat cgtttcaaac aagaggccga acgtcgtgag gtcgttcatc
     4321 aggtcttccg ggccgatttc cagggcggcc gcagcaaccg acggatcgac aaaataacgt
     4381 gcatcggaga tccggatgga atctttcgca cgaagattgg gattccaggc aggcatgtcc
     4441 tctacaacga acagacgcct caaggcattg aggtaagcgt agatggtgcc gttgtcaatg
     4501 gacgtcattt cattcgtgga cagatattct ttgatgacac ttgcggatgc ttgcgtacct
     4561 tggagtcggg catacgatcg catcagacga cgtgcccgat cttcgtcgcg cgtagtgccg
     4621 tcaacgcgcg aaatgtcctt cttcgttatc gcatcgacat agttgtaggc ctgatcaagg
     4681 gcggcatttc cggacagaga gacggcttgc ggccacccgc cgcggcagac gattcgggcg
     4741 atttcttcca actcaagcgg ctttgctgaa gcggtctgga cattcgcgcc ggagaacaat
     4801 tcccggaagg aaatctcgcc ggaagattcc tcggactccc atagcgacat ggggcgcatt
     4861 ttgagccagg caaaccgacc cgtacccgaa tgtttgattt cgtcttcgtt ggccgggacg
     4921 gaagaacccg tgaggatgaa acgaccgagg tctcccgcgt gatccacatc gtgccggaca
     4981 gcatcccaga ggacaggggc gatttgccat tcgtcgatca gtcgcggacg ggcacccgac
     5041 aggatgcgtc ccggatccat ccgcgccatg gcgatgttgt cgtctcgctt ctggggttcc
     5101 gccatgtaga tgacgctctg ggcaacttgc tcagcggttg ttgtctttcc acaccactta
     5161 ggcccctcga tcaggacggc ccccgtccct tctagtttac gccgaagaag cgaatcgact
     5221 acgcgatcct tgtatgctct catgagaaaa atcttagcag aacgatccat cgaaaacaag
     5281 gccgttcctt catttgtcct aaattcattc cttcatttgt cccaactcga ttcgtccatt
     5341 tgtcccaaaa ttgcaggaac ggcccgccgg ggacgacccg ccggggacga cccgccgagg
     5401 acggcgggct ccagggtacg gattcccgcc ggggacggcc cgcaccgtga acaggggatg
     5461 aacaatgcgg atacaagagg aaaaacaggg taatcggcat atcgggttgg ttccgggttt
     5521 cgcaccatga aacaaatccc ccgcgccatg ccaaaaaagc cattcccctt gatgcgtgaa
     5581 gctcggagtg atatcctgtt tccatctttt atccatccgc ttgggggtat aacgctcaag
     5641 ggcgtaatca tgtcaaacct ttctcctaca cccggttcct cccgtatcgt caagcatctg
     5701 cggacccgtc tggtttcagg tctgctggtg ttgatcccgt tggccatcac cctgttcgta
     5761 ctgaacatct tcctcaactc cctcaccgcc tttgcccggc ccctgctcaa acccctgctg
     5821 ggggtgttgc ccgaatccgt tctgacgctg atcgcgttga ccctcaccgt gttgctgatc
     5881 tatctgaccg gtctggtcac cacccatatc gtgggccgcc gcctgatcct tttcggcgag
     5941 cggctgctgt tgcggctccc ggtcgtcaaa accatctacg ccgcatccaa acaggtggtg
     6001 gaaaccttct cctcgcacca gaccacctcg ttccaagcgg ttgtcatcgt gtcgttcccc
     6061 cgccgcgaat cgctcgcgat cgggttcatc accggcaccc tgtttgcgcc ggacaaccgg
     6121 aaactctatc gggtcttcat tcccaccaca cccaatccga cctcggggtt cctgcttctg
     6181 atgtcaccgg aagatgttca attcacggac atccccatcg aagagggcat taaaatgatc
     6241 gtctccggcg gcatgctcgc cccggaaaag tatgcggtgg tgccggataa ttgtccatga
     6301 acaaaaccgc accgtctgcc aagctggcgg tgtaatctcg tcgaatcacg gaggcaaaat
     6361 ggctaaaccc cgtcgcgaag tggagatcac gctacacagc atccgcgttc cggtcaccgg
     6421 cacggggctc gataccgaca cctcccacct gctgaccgcc accctgatct ggccgcgtct
     6481 cggcgtggcg ggcaaaagcg ccagccaacc ctgcgcactg caacacggcg aatcatcttt
     6541 cgaaaccgta cattggggac ggcgcattct ctttaaagag gcggtggagg gccgtttcgc
     6601 tttgggactg gtcctgaccg aggcgctgga tgacgaagag atggagaagt tcctgcgctt
     6661 ctggaccggc gccttcctcg cggtcggcgc cgacctcgcg ggtgccgcgg cacccgcccc
     6721 cgcaggaaaa ctcgccgccg cgccactgga ttacgcggcc aaggcgatcg ccaaatatcc
     6781 cggcgcactg ccgctcgtcg aggggctggt cgagatcaac gccgccgaac tgccgcccgg
     6841 cggccgggag cggttgctga ccgtacgcct gaccgcggcc cgcaccttgg cgcgcctttc
     6901 ccgccgcacg gtcaacagcc gcccgcgcgt gagccgctcg ctcttgctgg agaagggggc
     6961 cgacaatggc gaggtcaccc tcgccatccg ttccctctga ttcacttggc tgtcacaaac
     7021 gccggacgcc gtccgcggta ccacgccagc atgaccgggg tggcgatgaa gatcgtcgaa
     7081 tacgtgcccg cgatcagacc aatcatcatg gccagcgcga aatcgttgat cgatccgccg
     7141 ccaaatatga acagcgtcac cgttgcgatc aacgtggata acgaagtcag gatcgtgcgg
     7201 ctcagcgtct ggttgatggc gcggttgcac aactccggga aaggcatttt cacgtcctta
     7261 cgcatatcct cgcggatacg gtcgaagatc acgatggtgt cgttgatcga atatcccacg
     7321 atcgtgagca gcgcggccac aaccgtcagg ctcacctgcc gcccgaacag gctgtaaagc
     7381 cccagcgtga tgaaggcgtc atgcgccaac gccaccacac cgccgagcgc gaagccgaac
     7441 tcgaaccgca gggagacata gatcaagata ccgatcagcg agaagagaat cgccttggcg
     7501 gcggataact tcaggtcacc gccgatctcg gaacccacat cctcctcgcc cgcaagggtg
     7561 aaacggctct cgggcagttc cttcgccaac gccttctgga tcactttgga ggcgctctcc
     7621 tcaccgatct gggtcacgct ggttttcacg agcaacaggt ttcccgaacc gtcgagggtg
     7681 gactggtact ggatcgtggc gtcgtttttc accgcatccg tgaccgcctt gcgaacggtg
     7741 ccgatatccg ccttctgctc gtaggtaaag gtcatcgaga cgcctcccgt gaaatcaacc
     7801 gccatgacgc tggcaggatc tttcaatcct cgggcaaaga agatcgcgag cgtgaccacg
     7861 atgatggcca gcgacgtgta tccggtcggc ttgccccaac gcaaaaagtc cacattcgag
     7921 ttcttcatga actgcagcat cttgtagggc cgggtgcggt tttccggcac ggtggcgttg
     7981 aatatcaggc gcgtcaccac caaagccgtg aacatactga tgatgatacc ggcggtcagc
     8041 gtgatggcga agccccggat cggccccgcg ccgaagatga acaggatcac ggcggtcaac
     8101 agcgtggtaa tgttggagtc gaagatcgcc agaaacgccc ggtcatagcc tgccgccacc
     8161 gcggcgcgcg ccgatttgcc cagcgcaaac tcttcacgca tccgctcaaa gatcagcaca
     8221 ttggcgtcga ccgccatacc gaggctcaaa acgatacccg cgataccggg catggtcagc
     8281 accggcagat ccaacacccg cttggcgatc gccgtgtctt tgacgaacac acccagaatg
     8341 ctggaggcca tgatcaatcc ggccggcagc agcagcaggt caagcagcag cgccacattc
     8401 gcgatcaatc cgcaatacgc gtaatagacc agcatgaaga gcgcgaccag cacggtggcg
     8461 aggatcgatg cttggatccc gctccggatc gcgtccgcgc ccagcgtgga ctcgaccgag
     8521 cgcttctcca gaatcttcat cggcgcgggc agagaaccgg cattgaggat gttgcgcagc
     8581 acggcagcct cggaccagga gaaattgccc tcgatcacag cgcggccgtt ggggatttcg
     8641 gtcttcagca ccggcgcgga atacagggta tcatccagca cgatcgccag acggcgtccc
     8701 acgtcgctgt ctttgttgcg cgaaccgcgc ggcgcgtacg ccttggtcag ccgggcaaaa
     8761 tcagcccctc ccttggcatt gaacaccagc gagacatgca cgcggccggt catcggctcg
     8821 atctccaccg aagcgctgga aagcgcgtct ccggtcattt cggccttgcg cagcacatag
     8881 atcggccggt aaatcacctg cccattcgcc gtctgctcgc gttcgagcat gaaagcgtaa
     8941 cgggggtccg gcacctcgaa cattgagaga cggtgcatat actcggggtc cttcaccagc
     9001 tcggtatagt tcggggcgcg cttatagccg cggccgtcgt cagatgaaac gtacccttcc
     9061 ggcaaacggc ccgaggcgag caccttgtcc acaagctgct cgttctgctt gtgcaccagc
     9121 ttgaattcaa gaaaagccgc gctctggata ctcttttccg cggcatcccg ctgctcggca
     9181 ctggcccccg gaagctgcac cacaatgcgg tgattcttgc ctccctgaat gaccggctca
     9241 ttgacgccca gcccgtcgat gcggttacgg atcacctcaa tggcccgcgc atccgcatcc
     9301 ctgagcacag cgttcacctg attctcgacc tccacgtcgg tgagttccgt gtccgcggcc
     9361 gcccttacat tggcgcgcag tttcgcctca tcgatcgcca ccgtgaaact cgtgccgccc
     9421 gagagatcca gccccaaccg gatcttctcc ttcggcggaa tcacaacata ggcagacaaa
     9481 acagttagaa aagccagtat cagccatttc cagagatcgt ttctactcat ttcagcaact
     9541 ccacaattta cccggaaccc ggaaagggtc aggagaaaga tttaaattat caaaaagccg
     9601 tggccatttc acccaagaaa tgaaaaaacg gctcacctgc cctcttccag agtggcgaca
     9661 tccccctctt tcaacacccg cgtgaccgcg ccgcgcgcga cctcaaccat caccttgggg
     9721 gcgatctcaa ccacaaacgt ggtttcgcgc gcctcgctga tggtgccgat cagacccccc
     9781 gcgaacatca cccgctctcc ggcgcgcaat tcggcgatca tgcgcaaccg ctccttctcc
     9841 ttacgctgtt gcggccggat catcatgaaa tagaaaagac cgaacaccag aaccatgggg
     9901 atcagcatcc cgccaacccc gcccgtaccc tgctgcgccg cctgcgccca aaaccaccct
     9961 gtcgtcatca cgtatccttt ctcgctcact ccaaacccgc gcgccaccac ctgtgccgcg
    10021 cgcgctttcc ctcaccctgc ctgcggcgcc cgggcgccca tccggacggg tttccggccg
    10081 tccggccgca acacggcggc gagcgcgcgc cgccattcac cgaatgcgtc gttggcgatc
    10141 gcctcgcgca tctcccgcat gaacccgagg taacggtgca tgttgtggat cgtcagcagc
    10201 cgcaccccca aaatttcccc cacgttcagc agatgccgca cgtaggcccg cgaaaaggtc
    10261 cggcaacaat aacagtcgca cccctcttcg accggccgcg tatcggcctt atggagtccg
    10321 gccttcacgg ggtactgacc ggtccgcgtg taggccgtgc cgtgccgcgc cacacgcgtc
    10381 gggatcacac agtcgaacat gtccgccccg cgcgccaccg attcgctcat ctgatacaga
    10441 tcacccagcc ccatcacata acgcggccgc tcgcgcggca agccgcgcac gccgtcctcc
    10501 actccccgca acatcacgtc ctcgggctcg cctacgctga caccgccgac cgcatacccg
    10561 tcaaagccca tggccgccag ttcccgcgcg caccgttcac gcagggcagt gtactccccg
    10621 ccctgcacaa tgccgaacac gagctgtccc ggcgcgcgtt cctgctttag gcagagagcg
    10681 gcccatgata gggtcttcgc cacagactgg caagcgtatt gttcgtcaca ggggtacggg
    10741 atgcactcgt caaaacacat cgcgatatcc gaacccagcg tgcgctgcac agccatggct
    10801 tcggacggcc ccagaaagaa cgacgccccg tcgatgtgcg aacggaattc aacgccgtgc
    10861 gcgcggattt tgcgcaggga gttgaggctg aaaacctgga atccgccact gtcagtcaga
    10921 atggggccgt cccacgccat gaaccggtgc agaccgccgc aggcggacac gacatccatg
    10981 ccgggccgca gcagcagatg gtaagtgttg cccaagatca cggacgcgcc caaatccgtg
    11041 aggtcgcgcg gctccagcgc cttaaccgta gcctgcgtcc ccaccggcat gaagaccggc
    11101 gtgtcgatct gaccgtgcgc ggtccacagc cgtcccagcc gcgcacccgt atggcggtcc
    11161 tctttaagaa tctcgaacac ccccggcttc tgtcgcatcg gttccacaaa aactcccaca
    11221 ctcccttgcg gatcatccgt tcaaggctgc aacgggctgc ccaaaccctt cttcgatccg
    11281 ttcgtcaacc cgctggttga aggcaagcag cggcgcacta agagccgtca accccaacgc
    11341 ctcgggcggc tcgccctcgt ccagcagcgc tttagcgatc tccgcagccg tgaggctgct
    11401 gccgcagcgg gcgagcagat agtcgcccgg ccgtcccgcc acctcggcca gaatgccgtg
    11461 cctcgcgaga tcctcaagga tgtctttgac aaagcggctg gaaatccccc gacgccgggc
    11521 gaacgcctcg gccgcgaacg gcccgcccgc cggatccttg gcgcaatggg cggcctcggc
    11581 acagagggtc agcgccaaca gcaggcgcga acgcagactg gcccgcgacg cattctgctc
    11641 cagcacatag gtgtcgcggt tctgcaccgc aaaggcaatc tcggagccca acaaaatgat
    11701 ttgccaactc gtgtataccc acatgagcag aatcggcagc accgcgaacc cgccgtacag
    11761 cgcgctgtat ttggcgatcc ccacctgcag catcgcacac agtttgagcc agccgccgaa
    11821 cagaaccagc gtcaccagcc cacccaccag cgccgcgccg gtctgaaccc gcacattcgg
    11881 catgaacccc agcagaaagg cgaacgtcag tgtgccgacc gtcagcgtga tcccgctctt
    11941 cagcagactg ctgttcacaa ccatccggac cccatcggag accgcccccc ccacagtctt
    12001 gtccattgcg tccgtgatca tcttcgcgat cggcatggag gaaaccacca tgatcagaaa
    12061 gggcaggatg atgatgacga acaggtagtc tgccagtttg cggaccagca cgcgcgaacg
    12121 ttcgacgccc cagattcgat tgaaactatc ctcgaccttg ccgagcatgc ccaccacgga
    12181 ccagatcaac ataaccgcgc cgatcccccc cagcgttccg aaactgatgg agttcacctg
    12241 atcgaacaac tggtccgcga tctccctgac ctgagttgaa aaggctttcg tcacttcttc
    12301 gtggacttgg tttttttccg cgtccccctc cgcaatctct gcggtccggg catccaccgc
    12361 cgcctccatc tgcgacatcc acgcgttgag atgacgatcc agctgctctt tggccagatc
    12421 cgcgccgccg aaggtccgcg ccaacgccaa agccagcacc agaatcggaa ccagccccat
    12481 cagcgtgaaa aaggtcaacg aggccgcatg caacgaacag gcgttccgcc tgaagccgtc
    12541 cacgaccaag tgccccatcc gtagcaggta caccccgctc cggtacaacc aaggcagttc
    12601 actggagcgc atctcccata agtcgtgacg cagaaacgtc gtcacccgtt cccacaccgt
    12661 cttcttcacg tccgtggtgt cactcatcct gctccctcct ttcgcccggg cgcacggatc
    12721 cggatcgcct tgtcagccac cggacagaca tactcgcaat acccgcaccc cgtgcacact
    12781 ttcgcatcta ccgtcacccg gctcaccaga tcaaccggat cccaagcgaa atccaaggcc
    12841 gcatccggac aggccgccac acacgatccg cattcacgcc cttccgtcag caggcatatc
    12901 gcggggttca cctgcgcgca ccccatcgga ttccggaatt tattttgccg cgtaaaccga
    12961 gggatcgcac cggtggggca gacctgcccg cataccgtgc aggtggtggg gcagtaattc
    13021 tccccgaacc ggacttccgg cgccagagcg ccggccagtc cggcacccac cccgccgcca
    13081 tgcaaaatat ggctggggca cgcccgcacg cagttgccgc agcgcgcgca caaccgcgta
    13141 aaccgcgcgg aggtcccgct cgcgggcgga cggattaccc cggcccctgc cgccgcgcgt
    13201 tccggcggca acaacagccg gtagaccgca cccgcgccca accccaaaaa aatcctgcgc
    13261 cccgcccccg taccgcgtac cgtgcgggac gcctcgtcct tgccgtccgc ccgttgccat
    13321 gtgaccgcgg cgcgcagcaa atcctgtgtc gcacccaacg ggcagagcct gccgcaccag
    13381 aggccgggcg tcagaagcga catcaggatc agcagcggca aacccgctgc cgccatgctc
    13441 tcccacgcga cgtcgcctgg ccggaagaaa gcgaaaaacg cctgaaaaat caccagcgga
    13501 tccaaccacc cgaacagcgg ataccccaac accgcagccc ctgtgccaac cagcaccagc
    13561 cccaccccga tccgcggcac ccgccccacc cacgcccgcc gccgcgcacg gaatccaacc
    13621 ccctcgaaac aggtgcctgc cggacagatc cagcggcaaa agaaacgcgg gaggaaaaga
    13681 cacacggcag ccaccgccgc cgcgccccac agcaaaacac tgccggcacc ggccgccgcc
    13741 gcgagcagtg ccgtgaaggg actcagcgag gggaacacca ccgacagcca agcaggaccg
    13801 accccctgca gggcgcacac cgcagccgcc acacccaatg cgacccgcac cgccgtgcgc
    13861 acgaaccccg tgcgccgcgg cttctccctt cctcgctctg acatacccgt atcataccga
    13921 cacttcgcgc agagcgcaac gtccggcgca ttcggagaac gcgtcacttc gtgaaatagg
    13981 cggtcgcgtt ctgcggacgg atttcggtga gcgtgccgat atagtggcga agcgagattg
    14041 gctgataggg atatttcgca atcgccgcct cgtcgatctc cacgccgatc cccggacgat
    14101 caaggggaat gtgcatgtac ccgtcgcgca gcgtgacggc ctcatcgcag atctcgcggc
    14161 ggtacggcac atcgctcgcc atggtctcca gcagataaaa cccgggctgc gacgcggcga
    14221 gttgcagcgt ggcggcattc gccaccggcc cgctgggatt gtgcggacaa aacgggatgt
    14281 gatgggtctc ggcaatcgcc gcaatcttac gtaactcggt gatccctccc gcgtgcgaga
    14341 cgtccggctg cacgaagtcc gcgccgcgcg cctccagcaa cgcacgaaac tgccaagttc
    14401 cgtagaggcg ttcgccgaag gagagcggca ccttgacgcg gctgcgcagg tcggcaagtg
    14461 cctcgatcgt gtccggaatg atcggctcct cgaaccagag gatgtcgaac gcctccagcg
    14521 cattggcaat gcgcagcgca gtcggcagat cgaagcgccc gtgcccctcg atcaaaatct
    14581 ccgcgcggcc gttgaccgcc tcatagagca tttcgacgca gcgcatcgcc ttgcggaacg
    14641 cctccggctc aagctgccgg taggcggtac cgaacgggtc ccacttcaac cccttccagc
    14701 cagccgtctc tatcgcctcg acggccttcg cagcgaactc ttccggctct ttcgcgcctg
    14761 cgaaccagcc gttggcataa cagggcacct tgtcgcgcat gcacccgccc aacagccgcc
    14821 agaccggcgc attcagactc ttgcccaaaa tatcccacag cgcgatatca atcgccgaga
    14881 tcgcgctcat caacaccggc ccgccgcgcc agtaggcgtc gcggtaattg ctgtgccaga
    14941 tcgcttcgat gtcgcgcggg tccttgccga tcaggtcacg ctccagatcc atgcaggcag
    15001 atacgatcgt ctgctcctta tactccagag tggcttcgcc gactccccac aagcccggct
    15061 cgtctgtcaa cactttgaca aacacccagt tggtgcggta cgcgttgcag acaaacgttt
    15121 tgattccagt gattttcata aaaccgtgtc ctactctaac acgcggtcac ggttgaacgt
    15181 gaacagcatc gaacagaata tcgcgaccgc ccccagaacg taggcaaacg ccaacaccgt
    15241 gaacccctgc tggataccgg ccaccttgcc gtgcaggtcg cagaacttgc caatcagctt
    15301 gggcggcacc gtgcccagaa agaacgccgt catcgtcatc agcccgactg cggtggaacg
    15361 gaatttcgcc ggcaccacgt cgaacaatga cgcatgggtg ttgacctcga agagaccgcg
    15421 gaaaatcccg tatcccgcca ttgcgatcca tgtggccgtg atgccgttcg ccacgccgat
    15481 ccagtagatc atcggcgccc ccagcaacaa cgccgcaccc tgcagcaaca aacgaaaacg
    15541 cggatagcgt gacacggccc aatcggtcag caccccgccc gccatgatcg ccccgaacgc
    15601 cgccaagtgg tgatagagca tcgccccgcc cgccgcctgc gtgagcgtca tcggcgtcgc
    15661 ggaactcttg aacttctcca tcacaaaggt cggcgcccag aaaacgtagg cattgttcac
    15721 gaaaaccacg gcgcagaacc cggctgtcgc caacagcgcg ctgggcgtct tcaccagcac
    15781 gcggaccgat tcccagaccg acggcttatt gacggcggca ggcttgtccg gccggggctc
    15841 ggcaggcgcg tccttcagcc ggaagatgaa aacaaggccg agaaggacac cggcaccacc
    15901 gaacagataa aagaccgacc gccagccaaa ggccgcctcg accaaaccag ccagatagcc
    15961 gctgcacatc agcccgacat acaacgcggc ctgatggacg gaaagcgcca cggaacgcgt
    16021 ttccttgtgg tacgacgcca acagcgcgta agcggacggc gcataaaagg actcgccacc
    16081 gcccgtcgcc acactgcgga agagaatcag gccgagcacc cccgtggcca ttcccgtcat
    16141 ggccgtcgcg cagctccaaa aaatcaggct gcatgtgata atccacttac ggctcaggcg
    16201 gtcgcccata aacccggcta tcggaaccat caccgccaac gtcgcgaaca tccatgaacc
    16261 gatttcgccc atctggccgt cggtcaggcc aagttccgtt tgaatcggga tcaacacaac
    16321 gctgaacagg gcacggtctg cctgatggaa aaagaaagca aacgacagca gcgccagcaa
    16381 ctcccacttg tagctttttg aaggtttcat atatccacat ccaccggcat gtgttgcgcg
    16441 ctgcacccgg catccaccac cagacgttca ccgcaaatgt tgcgcgcggc gtccgaagcc
    16501 atgaaagcca cggcctgcgc gatgtcctcg gccgtcgcct ccatgccgat cgggcagttc
    16561 aagcggcggc gcttcatggt cgcctcgttg agtttatccc aacggtcggt atagatatac
    16621 cccggcgcga ccatgttcac gcggatcccc ttggggccga gatccaccgc catcgagcgg
    16681 gtcagcgcat ccaacccgcc cttgcttgcc acataggccg tgcggttccg gatcgcccgc
    16741 atcgagacgt tcgagccgag attgacgatc acgccgcgac tctcctgcgc cagcatcatc
    16801 ttgaccgctt cctgcgaaac ctggaaggtg cccaagaggt tggttttgac aaccgacagg
    16861 aaatacgcgg gctccatctc ctcgaacgcc ggcccaatgc cttggttgca ggcgttgttc
    16921 accagaatgt ccaatcgccc gaacttctcc cggatcacgc ggaacatggc gcggacctgt
    16981 tcggtatccg aaatatcggc cggcgtctcg gtgaactcgg tcaaacccat cgcgcgcaac
    17041 tctgccgcac ccttggccgt gctttccgga gatgaactgc acacgcagac tttcgctccc
    17101 tcgcggataa aaagcgcggc gattcccaca cccgtgttcc ggctggttcc ggtcaccagt
    17161 acaactttgc ctttaaaacg catcccttct gcctttctgg tttcacccat tgtcaattca
    17221 cgcctcattt tccgctacgg atttcgtaca accggtatcc cacctcacgg atccccgcga
    17281 tcgtctcggg atacggcgtg ttgcagcaat ccacaaaccc cacctgaaaa ttctcaccgt
    17341 cgaagcggcc cgtcgtggcc tgatcgccga actggtgcca gtgcacgccg acaacgtgcg
    17401 gatgttttaa tgccgacgtg acatactgca catagacccg gccgcgctct tcctgatttt
    17461 ccagcttgat cagaccggga tggaaaaggc cgcgatccag cgccccgaaa tggaactcgc
    17521 cgatgatcac cggcttatca ataccctccg gcaacttgaa gtcattgagg tggcgcgcgt
    17581 agatgttgta gctgatcaag tcgcagtgcc gggccgccac gcgcaccgcg aactcgggac
    17641 cccgtcccgc gaagcggcac cccatgtaga gcttgtttgg agccgcagcc ttgaactcct
    17701 cgcggatatt ccggaagtag gcgtccgcga tctccagcgt gaatgccttg agatccggtt
    17761 ccgccgattt gcccggacgc gtgcgggtcg tcaggaatgc ctcccaatcc gcatacgccg
    17821 tgccccacgc cgcgttgagt ttgtcaatgg tgccgtaggc cttccgcatg cgctcgcgga
    17881 acacgcgctt ggccgcctga tccgccggcg actccagcgt ccacgccgcc agatcggtct
    17941 cgccgcccca actcagctcg ttgtccacga aaaagccgaa gcaccacgga tcttgcatct
    18001 cggctttgtg ctcgttcatc agccgccgga catccgcccg gaaagaggga tcgaacgggt
    18061 cacggaaggg ccaccagccg tcatgactcc ccgcgatggg cttggatttg agttcgaacc
    18121 ggtcggtata gggcgtgcgg tccatcaggc aaatcttttt atccgaactg ttcgcgatcg
    18181 tgttcatgcc ccagctccgc aaccgccggt gcgcgagatc cgcaaaggtc tcgaaccagg
    18241 ttccgccata tttgcgcagc agattcgctg ccgagaaatc gtaagtccgc tggatgttgc
    18301 gtttgacgta atagggatag agcaactcgt cgcgcgtggt gtagaagagc gaaaaaggcg
    18361 atccctcgcg cggcggcagc ccctcgaaat agaactcccg gttgtccaac ggcgtgatcg
    18421 cggtcgaagg cgtcacgcgc accggcccgt gcgaccagta cagatatcca tcggggtcca
    18481 ccatccacca cttgccgtcg atcttctcga cgcggaaatg cccggtcgcc gcacgtttcg
    18541 gcccgtccgc ccatccaccg aaacggctcc agcccttcgg ccccggatgg gctgccaagt
    18601 ccgcctcttc acgccgtgcc gccgtcttca gctcctcgtc ggaatggatc tttcccggcc
    18661 acgttttatg cttaaactga ccgtacacgt cgatgaaggg gaagaactcc ttctccgtca
    18721 gtttcatcca agacggaacg tccgcgaccg gcccggtgtg cgccacgatt cgccggacac
    18781 cccacttcca atccagcttg ttcagccgca tgtaaaccgc cacgccgacc acgcgcgcgg
    18841 tgtccagatc cgcaaccacg ccagtcgttt gaaacggcgt ggtgcgcatg ccggtcagct
    18901 tgctgctgat cgcacgcccc gaaggcagtg tcggcggcag tgtgaccgta caggcgtgcg
    18961 cgcccttgcg cggcattttg atgcggtcaa caaacacgcc agtggacttg ccgggatccg
    19021 cgcccggatt gtcaaagcga accgtaagcg gcaactcccc cttcccgtcg cagtgctcca
    19081 actcgaaggt gacgctgttg cagcccgaca gatcccattc gccagcgatg cgtaccccgg
    19141 gataacccaa gttgcctttt gtctcaatcg cgagcaaccc gtccgtgagc gtgaacgttc
    19201 cgcccgaatg tgacgcgacc gccgacagcg gcgtccttgc ggcatcgaac aacaccacat
    19261 ccgccgccag acatcccatc gccagccata cgcaccccaa ccaactcaaa cgtcgcatat
    19321 cgagcccctt atcttttcta acacggcaaa accatacggt actataccgc acccggagcc
    19381 gtttccgcaa tctcgataat ccgaacccac cccctcgatt ttccgaacca tggggatcgg
    19441 gttccgacac gttccctgcc atccgcattg gcgcgggagt cgaaagcgcg ggcatccccg
    19501 accgcaatgg cggaccagcc ggaccggacg gatcagtcgg atccgttgtc ctgccaaaca
    19561 ggccggatgc cgcaggtcac ggtcatttgc aaatcacaac gaaacccttc ttcggcagaa
    19621 gatagcggat gatcacgacg ccgctgccgc ctgcggcatt accgccgccg ccgccgccga
    19681 gtccgtcgac accgttctgt ccgcccggtc cgccgccgcc gagtccgcct tgagacatcg
    19741 ccggataacc gctgccttta cggtaaccgg atccgccgcc agcataatag gtcagcgtac
    19801 cggaaatatc gaaagccaga ccgtccccgc caataccagg gctggtgccg ttactgttcc
    19861 cgccgaccgc accggctccg ccgccgccgg ccgccgcata aacatgagaa cactcgccgc
    19921 cgtcattgcc gaggttcccc tgctccccgt gaatcgcctg tccgggcaca tacgctcctc
    19981 cgctgatcca gattccgccg ccgccgctgc cgccggatgc aggtttccgg gcgtttccgc
    20041 tacgaccgcc gccgtgtccg ccaccgtagg cgacgaacct gacgaactcg gtattgccgc
    20101 cgttctcgcc cacattaccg ccggcaccga tcttcacctg atgggttccg gcattgagca
    20161 ccacgacatc acggtagatg agaccgcccg caccaccgcc cgcgccacct tgagcgggat
    20221 cggtatcgag attcactccg ccgccgccac cgccaccgac catcaggaat tccaccacac
    20281 cggcttccgg cagtgagaag gttccgtcct cagtgaaggt gtggatatta cagccctcac
    20341 tgcgcgtaac ggttccgccc gtcgcgttct cgatgtagtc gcgataaaat gatttcttcg
    20401 cgtaacggac gatcacgata ccgtcgccgc ccgcggcata cccgccgccg ccgccgccaa
    20461 gtccgtcaac gcccgcgacg tgtgaatcac cgccgccgcc cttgccgccc ttcgccccgc
    20521 ggttcggctc tccgccaccg ccgccgcccg cataccagac gttgtcaccg gtgatgtcga
    20581 aggcgagtcc gtcgccaccg tcaccgggga tattggcggt cccgctggta ctgtcgacgc
    20641 cgtcttcccc ggggccgccg gctccgccgc cgccgccggg agcgtagatg ttggcaccac
    20701 tcgcaccgcc gacatcgccg atgtttccgt ctatggcaaa aactgcggaa ccgccgggca
    20761 ccttatttgc ggaaatggta gaaccgccgc cgccactgcc acctgaagat ccggtgctgt
    20821 tgtaaccacc ggcaccgccg ccgtgggcta acgctccaag gaacgaactg tcaccgccgt
    20881 tcgcaccaac cgcgccaccg gcaccgacga tgatgtcgta ttcgcctgcg gttatacgtg
    20941 cagacgaaat atgcacgaga cctccggcgc cgccaccgcc gccggcgtag gtatgattac
    21001 cgccaccgcc gccgccgccg accatcagca cctcgacgca accggagccg gcaacggtca
    21061 acttgccgct ctgacggaag acgtgcacgg ttccattggt gagcagtatc ttttcgtcac
    21121 cgcccgtcag cagaaaatct ttcgtgtctg aaaccggagt cggcgtaaat ttgatgacaa
    21181 ccgcgccaca tccgccatca tagccgccag aaccgccgcc gccgagtccg tcgacgccct
    21241 tctcatagcg ggaaccgccg ccgcctttgc ccccggacgc aaacgtccct tgtctgtagc
    21301 cagcaccgcc gccaccgtag tagacaccgt aatcaccgta aaccgcaagc tcgataccat
    21361 cgccgccacc gcccggagcc gatccgccgc tcgttacgcc gggagcaccg gctccgccgc
    21421 cgccgccagg tccgtaaaga tgattgctgc cgccgccgtc attaccgtat tcgccttgcg
    21481 ccgcaatcga agcgccgccc acggccgtag cgttgtaagc cgttgaaccg ccgcccgaag
    21541 ccccggcccg cccttgccga gcctgaccgt tgttgctgta attcgcgccg cctccgccac
    21601 cctgggccgt aataccgaaa gccgttgtgt tgccaccgtt atcggcggtt ccggtcgcat
    21661 taccgatcac gcccgtagta ccgcccgagc cgatttcgat agggtaaaca ccggcggaga
    21721 ccgcgacatc ccgtcgcaga atcactcctc cgccgccacc gccgccgccg ccttgagaga
    21781 gatcgaccct gttcgcgcca ccgccaccac cgccgccgat gaggatgacg tcgatcgtac
    21841 cggaaccgct gatcatgata tcctgcgaat tggtgagcgt gatcaccgtc gcgttggtat
    21901 agtacgccgc cttcgcgata tctgcggaga tattcaccgc cgaaacggcc agcgcggaca
    21961 tcacaccggc aatcgagagc atcatttttt tcatcatcac aacctttcgt gtacccctca
    22021 ccccggccct tcccagagaa cgagggagaa caaaacgcac taaaaaaacc actttggtct
    22081 cggattgtcc aaaatataac attcgggttg cggattgtcg agtttacgcc cggcgttttc
    22141 gtgccccccc ctgtttgcgg tctcgtctaa cgaagatcag tttctcccgg tttgtccccc
    22201 gctcccacga agcgttgtct ggtcaggcgg atcgcatcac cgtgccgccc ccgatgagtt
    22261 gttacactcg acacgcccta cgtctcctgt tacgcttgtt tccgtcatac gggaaacgct
    22321 ccacggtgtc ctatgtttta tctgttcctt ctcttccatt ttgtttttct gctgttctgg
    22381 ctgcggttct ggtccgcacc ggagcgcgag ttttatttca atccgttcgt gtcgggcacc
    22441 atcaatctga ccgatcgtgt catcctgttc ctgcgcccag tgctcgcgat acccgaggcg
    22501 atgaccgcac tggtcatctt gattgtcgtg gtcctgttgc agacgctgct ggccgcacgg
    22561 ctccagtttc cgttgggtgt gaccctcggc acgacctttc atttcacgcc gcccgccgta
    22621 tccgacaacc ccctcctgcc gctcccgttc accctgctcc aaaacgctct gtttctggtg
    22681 cgtctctgga ccgtttatct gctggtccgc ctgatcacac cccccttccg cgtcacccgc
    22741 gcgaccgaag ccctggcctt cttcgcacgg ccgttctccc gtatcccgct cctgttccag
    22801 ccattcctgc tgctggcgct tcacgggttg atcgccctgg ctctcacccg cacaaccgaa
    22861 ctgcggatcc tgacccggct gacggaaacg cccgccgcga ccgacgtctc gcctttcctg
    22921 accggccccc tcctcctgca gctcctgaaa acaggctggc tcgcggtcct ctcgttttcc
    22981 gacggcctca tatttatgac gcgctcgctc tttgtgctga tcatcggcag cctcggtgcg
    23041 gccatcatcc aatcgcgcgg cctcgccgtt ttatgcagcg aggccgtgga tctcctgctg
    23101 ggccgcttcg cccggcgctg caccggcgcg atgggcttcg acttcacgcc gctcatctat
    23161 ttctttgttg tggatttcct gtacaacggc atctgccgcg gactgtacat gctgatccat
    23221 tccccattat tcaactgagg tgaagatgag ttgggtcgtt gaaacccgtg acgggtgcgt
    23281 cgtgacggtc aaggccacgc cccgggcaaa ccgttccgag atcgccgggg tcgatgcgga
    23341 ttggctgcgc gtgcgtttgc aggccccgcc ggtggatggc aaggccaatg ccgccctgat
    23401 cgcgtttctc gccaaagtgg cgggcgtgcc caaacgatcc gtcgtgcttt tgtccggcga
    23461 gacctcacgg ctcaaacgcg tggccctctg cggcgtgacc gccgcggccg cgcgtcaggc
    23521 cttcgcaaaa caatgaggcc cggcggcacg cgccgcccgt tcactgctcg tccgccgcgg
    23581 caggttccga ctgccgggtt ttgaaagcct gcggcacaac caacacgggg catgtgcttc
    23641 tgcggaccac tttaccggtc acattcccca gcaaccactg cgtgatggcg cctcgtccct
    23701 gcctcccgag aacgatcaga tccacatctt gggatgcggc gaaatccaga atcatgcggg
    23761 cggcgtcgcc gatccgcatc gcaatctgaa acgtgacatc ggacggaacg cgcgggcggt
    23821 aaaccgtatc aatcatacgg tcgatatcgg cgcgcgcctt cgcgtccata tcgccgacct
    23881 cgtaaatgta ccccttccaa aattgcgcgt ccggctcggg aatgacgtgc agcaggtaga
    23941 gtgtagaccc ggtattccgc agggcggcat ccaccgcgaa atgaaacgca aaatccgcat
    24001 tcgcggagaa atccgtgcaa aacagaatct tccggtaatt tccgtgccgt gccgttgatc
    24061 cgtccatacg tcctccttcc cgaattgact atcgcaccac cccgggcagc catgtggcca
    24121 gaccgggaaa aagcatcagg atcccgcacg ccacgatcag cgcgaacagg aacgggatcg
    24181 caccgcggaa aaccgtctgg agcgggatat cccgtgcgat accgctcacg acatagacgt
    24241 tgacccccac cggcggggtg atcaccccca tctgcgtcac cagcacgatc atgaccccga
    24301 accagattgg atcgaacccc aaccccatga ccaccggata gaaaatcgga atggtcagaa
    24361 ggatcagcgc cagcgagtcg acaaaacagc ccgccagcag ataaaagaaa aggatgagga
    24421 gcatcaccac ccatgacggc aacggcaggg ccgcaagcca ggcggcgaac tgcgtgggca
    24481 gtcccgtaac cgccagaaaa tggccgaaca tcgtggcgcc cgccacaatc accagcacca
    24541 tacatgaggt ccggatggtc tcctgcgcgg cctgccggaa cagggcccac gatccgtgtc
    24601 cgccgcatac cgcgatcgcc acactgccag ccgcaccgat cgccgcgccc tccgtaggcg
    24661 tgaaccatcc cgcaaacatc ccgcccatca ccagaacaaa gaggatcatg gtctccgtaa
    24721 ccccggagag cgaacggaaa cgcacccgcc acggggtccg caccgctttc ggggccaacg
    24781 cagggttggc caagcacaga acatggatcg tcagacagaa gagcagcgcc accacaatcc
    24841 ccggaagaat gcccgcgatg aagagcttgc cgatggactg ctcggtgaga atcccgtaca
    24901 cgatgaaaac cacactcggg gggatcagca tcccaagccc gccggctgac gcaaccgtac
    24961 cgctggccag ggcgtcatca tacttgtgct tccgcatctc gggcaatgcc accgcagcca
    25021 tggtcgcggc agtggccggt ccggagccgc agatcgcgcc gaaggccgcg caggcaccga
    25081 cggtcgccat agccaacccg cctttcagcc cggagagcca gctgttggcc gccgtgaaca
    25141 acctcccgct gatcccagtg tgaaacgcca cctgtcccat gaagatgaaa agcgggatca
    25201 cggtcagatt gtaactggag aacacatcgt acagatccgc cgaaagcatg gccagcgccg
    25261 ccttgccgga gaccacggcg gcatatccga caacgccgac caaccccatg acgatcgcga
    25321 cgggcatgct gcagagcagc aagatgaaca gcgcgacaca cccgaccaca ccgaacatca
    25381 caggcgtcat gacttcacca tctcccgtcc gggatagacc aaatggaaaa ccaccaccaa
    25441 ggccgtcacg gcaaacgcca cggccatcac ccacggcacc cagaagatcg gcacctcgat
    25501 cgtatccgaa acctgccggt tgcgcaggaa gcgcaaccca tagcccacgc attccgtagc
    25561 ggtgagtaaa aacgccgcga tcatcagaag ccgcatgacg gaatccacca caagccggcc
    25621 cacgcgattc aacttcagga agaaatactc gatcgccaca tgccccttct tggcggtggt
    25681 gatcggcagg gaacaggcga ccgtcaccgc ggcgacaata cgcaccagat catagacacc
    25741 tttgaccgga tgacccgcaa gccgcagcac cacgtcgacg cacaccaacg cggtcatgac
    25801 cgccaatccg caacaggcga ttcccgtcat gaaccggcag aggccgacca ccgactgcca
    25861 aaaaggaccc aacggctttc ggaccggttt ttgtgcagga ctcatcgtga ggagccctcc
    25921 tgagcctccc ggaccgcagt ggtgagccgg ttcagaaaat ccttgccggg gaggcccttc
    25981 gcttctgcac gttccgcata ctcccgcaga agcggctcga cctgtttccg ccacaacacc
    26041 tgctccgacg gatcgagcgg aaccacctcc cgatccagcg accgggcaaa ggccagcccc
    26101 tcgtcatccg cctcgttcca cgcctgagcg tggcgctcca cccactcctt gctgacatct
    26161 tccatgatct gcttcacctc atccggcagc gaggcccagc gcaccgggtt gatcaccacg
    26221 aaaaaggagg tggtataccc gatacagcgc gagtcggtga tattgcggat cacctccccc
    26281 tgtttccatc ccttcagcgt ctcgaccggg cagaaggtgg catcgaccac gcctttctgg
    26341 agcgcctcgt acgtatcgcc ctgcggcatg ctcaccggcg aaccgcccag actctccacc
    26401 actttcgcgc agaacccggt cgcccgaacc ttggccccct tcaagtcggc gagcgtgcgc
    26461 atcggacgtt tggaggccag aataccgggg ccgtgcgcat gcacgtaaag cagtttcaca
    26521 tcgcggatct ctttcggtaa aaaggcgtgg cagagttcgt tcacgatccg tgtcgcgctg
    26581 accccgtccg catatcccac aggcagatcc aacccctcca gcaaaggaaa ccgtccgcgc
    26641 gtgtacgcga aggcgctcat gccgatatcg gagatgccat ccaccacacc ctgatagcac
    26701 tggtcagcct tcgtcagcga gccgccgcaa aagaccgtga tccggacctt gccctgtgtg
    26761 cgacgcccga tctcttcggc ccacgcctcg gcgagtttcg cctgtttgtg cgtgggcggg
    26821 aagaaaacgc tgtacgtaag cttcaccggc cgcgatccgt ccgcagccgt ggtttcctgt
    26881 ttgccgcacc cggccgcaac gatccatccc gcaaacaaca ggaacaggac cgcaccatac
    26941 tggctccgac tcatttcatc cgccttcctt ttgtccatgc gcattttcag cctaaaaacc
    27001 cttttcatat cacactaagg cacaaaggtt ttttcatgac aaacccttcc cattcgcacc
    27061 cttcgcatga aaaacgactt gtttattgcg tttgttgcgc tcatgcctat tgcatcgcgt
    27121 cggccagcgc gcggccgaac gcactgcaag agacttcaac cgccccttcc atgaggcgcg
    27181 cgaaatcgta ggtcaccgtc ttattcgcga tcacgcggtc catggcgctg atcagtttgt
    27241 ccgccgcctc ggtccatccc atgtaacgca gcatcatctc acccgagagg atcaacgagc
    27301 cgggattgac cttgtccaga ttggcgtatt tcggtgccgt gccgtgcgtg gcctcgaata
    27361 ccgccacccc ggtcgtgtag ttgatgttcc cgcccggcgc gataccgatc ccgcccacac
    27421 acgctgccag cgcatcggaa atgtaatcgc cattcagatt gagcgtggcg atcacatcgt
    27481 attctgcggg gcgggtcagg atctgctgca ggaacgcatc cgcgatgcaa tccttaaccg
    27541 taatctcccg gccggttttc gggtttttga accggcacca cggtccgtca ccgatgagcg
    27601 cggcaccgta cgcctcgcgg gcgagctggt agccccagtc gcgaaacccg ccctccgtga
    27661 acttcatgat gttgcccttg tggaccagcg tgacgctcga gcggtcattg tcgatcgcgt
    27721 aatcaagcgc cgccttcacc aatcgccgcg tcccctctat ggaaaccggc ttgatgccga
    27781 tcgacgaact cccggggaag cggatcttct tgacccccat ctcgttttgc aggaacgcga
    27841 tgaccttgcg cgcttcgtcg ctctccgcca gccattcgat cccggcatag atgtcttccg
    27901 tattttcacg aaagatcacc atgtcggtca gctcgggccg tttcaccggc gagggcacgc
    27961 cttggaacca gcgcaccgga cgcaggcaga cataaaggtc cagctcctgc cgcaacgcca
    28021 cattaagcga acggatgccg ccgcccaccg gcgtggtcag cgggcccttg atcgagacga
    28081 ggtacgcctt gcaggcttcc agcgtttcat caggcaacca ggtgccggga ccgtacaccg
    28141 cattcgcctt ttccccggca tagatctcca tccaggcgat tttccgtttt tcgccatacg
    28201 ccgtctgcac cgcggcatcc catgcgatcc gtgccgcacg cgtgatgtcc ggccccgtgc
    28261 cgtcaccctc gataaacggg atgaccggac aatccggcac atgcagacgc ccgtcgtcgc
    28321 ctcgggtgat cggttcaccg aaagagggaa tttgaatatg ctgataagcc atgctgccgc
    28381 gttctcccat gttaggttcc gtttgaactg caatcgttac tcagaagtat aaagcgacgc
    28441 cgctccgact gcaatgcaaa gatgcccctg tcacacgtac ggccgttgcc tgcggatttc
    28501 gataaacgaa agcctggtat ggtaggcgcg gaaccgcgcg gcgtcaagcc gcgccgcatg
    28561 ggaaacgcag gccggacgat cctgaaaacg cgattgcatt cgggcaccaa accgataaac
    28621 ttgttgcttc ataacagaag gacgccgcaa tcccgcaacg gatcgtccat acggtcgtca
    28681 tggaaaagaa gcgtccggaa ccacgcccgg aataccatgc ccacaatccg cattccttac
    28741 caccggggtc atttggaggc gacgcttccc gcaaacgtgc gcctcaccct gttagagccg
    28801 accggcaccg catgcacggt ctccccctcg gaagcggaac agcgccaacg ggtttgcgcg
    28861 gcgttggatc atcccgtcgg cacgccgcgg ctcgaaaccc tggccgcagg caaagcctcc
    28921 gccgtcctga tcaccagtga tcacacccgt ccggtgccca gccgcatcac gctgcccctc
    28981 ctgctggagc gcctgcggcg gggcaacccc ggcatcgcga tccaaatcct cgtggccacg
    29041 ggctgccacc ggccaacctc ggatgcggag ctgcgcgaga aattcggcgc ggaactggtg
    29101 gcgcgcgaaa cgtttgtgat gcacgactgc accgacacgg caaatctccg gatgctggat
    29161 caactgccgt cgggcggcgc actctggttg aaccggctgg cgctggagac cgagctgctg
    29221 gtggccgaag gtttcatcga gccccacttt ttcgccggtt tttccggagg gcggaaaagc
    29281 gtgctgcccg gcatcgccgg acgggaaacg gttctagcca accactgcgc ggcattcatt
    29341 gccgaccccc atgcgcgggc agggcaactg gagcggaacc ccatccaccg cgacatgctg
    29401 ttcgccgcgc gccaggcgcg tctggcgttc atcctcaatg tcaccctcca tcccgacaag
    29461 acgatccgca cggctgtggc gggacatgcc gaggcagcgc acgaacaggg atgccgcgac
    29521 atgcggcgcg cggtctccgt gccccgcgcc gaggcggaaa tcgtgatcac cggcaatggc
    29581 ggctacccgc ttgaccagaa tctgtatcaa gcggtcaaag gcatgaccgc agccgaagcg
    29641 tgctgcgtgc ccggcgggac catcatcatg gtggcaggct gttgcgacgg ttcaggcggc
    29701 gaatcttttt accgcgcact ggccggggcc cgaagtcctg cggaactgct ggcggaggtg
    29761 ttgcgcgtac cgcaagccct caccccgccc gaccagtggg agtatcaaat tctcgcccgt
    29821 atcctcagcc gacaccgcgt gattctggtg accgacctct gggacgccga cgcgatccgg
    29881 gctctgcaca tcacgcccgc cgcttcgctg gacgaggcac tggcgctcgc ctggcacgag
    29941 cacgggactt cagcgcggat caccgtgatc cctgacggcg tctcggtgat cgtggaacct
    30001 aaaaagagca gttgaacctt cacctctctt aggtttcaag gtttctgggt ttcagggttt
    30061 ctgggtttct ggtttctggt ttctgggttt catccaaatc ggggtcggga tcgctatcgc
    30121 tatcgggatc gattgtttaa ctttcttggg tttcagggtt tctgggtttc tggtttctgg
    30181 tttctgggtt tcatccaaat cggggtcggg atcgctatcg ggatcgggtt tcaggggttc
    30241 aagggtttca tccgcagatt acgtaggggg acgcaggttt tcgggggcga gcgtcctcac
    30301 gagccgcatg gcttgatacc gtcgtcattg cgttctccat cccgtgcagg caagcgttcg
    30361 tttgcctgcg aacgagcaga cgcgcatccc ggttgccgcc ctattcccct gcctcgcctt
    30421 taccagacgg ctgagcctgg tctcacgcgc gcggcgtacg cgatattcgg cttgctatca
    30481 ggcggtaggc cagagcggcc gccagcaggg cgcctgtgag ttgcaacgcg cggcggaaga
    30541 ggtggtcaac caacgcttcg gtttccgcgc gagtgcgccg gacaacaggg tcgagctgtg
    30601 ccgccagcgc ttcacggctg ttggcgccga gggtctgatc aaaggtctgg agcagttcgg
    30661 tcagacgcag cgcggaagcc tcaagccgtt cggccgcctc gctgtagtcc tgaatgcgga
    30721 agggtgtgcc ctcctccttc gccgccacct gtgccggcgg ggcgtcgctc tccgagccgt
    30781 caacccccat ccgttccagt acgccatcga acgtagcgag catggtggtg gcattcgtgg
    30841 cgagtccgga accggccagc agagtctccc gtacgccttc cacgagaggc gtgagagtct
    30901 tttcctgctc gtcgcgcaac gtctttacca gggcttcacg ctcggcggcg acctgagccg
    30961 gcagtttctc ggctgtcgtg gcgaggcgcg cggcggtctc ggcaacgtgg gccacggtct
    31021 ccccgatgtg gttcgcagcc tgcaccaaat ggggcacgtt ggtggtccac atctggatga
    31081 cgggctgttc cagcgtcgtc tgg
//
LOCUS       DAOSRB010000009        29861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1707_length_29861_cov_6.803496, whole genome shotgun sequence.
ACCESSION   DAOSRB010000009 DAOSRB010000000
VERSION     DAOSRB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 29861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29861
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_29861_cov_6.803496"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="PLZ74_01055"
     CDS             complement(<1..1564)
                     /locus_tag="PLZ74_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR96944.1"
                     /translation="MRRFPRYRLSPLTRKRLERFWRIRRARVALILLTALFAVSLCAE
                     WLCNGRPLFLRVNGRTWLPFLQRLTLRDLEGPDAEATRVNFRAFVRSPAFTTNPLNRV
                     VWAPVPYSPGDVVDAGTLSRFRTVHVSVVPEIRVGRLNLLPDGTVIRAEGCEPFFPGE
                     ARVNGVVLDRHWHVPETLRASLDARAAGRPAPAQRFLLTHLRQSDLRARVTLPETAGA
                     AGPPRLMFRQIETPDLPVQVRFRRDDAGTVYPVTHRTWRQIPATCQPEILALASAAFA
                     GTAPTARIPWKDRHATVACGLTEVAWPFPPVRGHWMGIDSAGRDVLVRVLYGMRTAML
                     FGLLLALWAVLFGVVIGAAQGYFGGWVDILGQRLTEIWSALPFLYVMIFIGSALGRSF
                     SLLLLCYGLFNWIGISYYLRAEFLRLRNRTFVEAARCQGLSSARIIFNHILPNALTPL
                     ITLFPFTLIGAIGSLAALDFLGFGLPPLTPSWGELLQQAQQFRWAWWLILFPSLALFT
                     VMLLAVLIGEGLR"
     gene            complement(1561..2601)
                     /locus_tag="PLZ74_01060"
     CDS             complement(1561..2601)
                     /locus_tag="PLZ74_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR96945.1"
                     /translation="MNRFAYLVRRLLLVIPTFLGITMVCFALTRFLPGGPVEIQLMKM
                     RNLGGANDPGSAAVGQVTEEYRQELTRQFGFDRPLPVQYYDWLVRKRMGLLIASYDYP
                     NRTAWQLIASRIPVSLWFGITGFVLSYLICIPLGVAKALRHRQPFDAISSLIVFAGYA
                     IPAFALGMVLKMFFCGTVEGLADLFPLGGFESDLPATAGWGARMADRAWHMALPVTCY
                     VAGNFAVLTLIMKNALLDQIASDYVRTVLAKGATRRRAIWGHAFRNALIPVATGFGSI
                     LGVLFAGSIIIENIFEIPGMGRLSWEALTGRDYAVFLALLALTSLLQLAGNLLSDFCY
                     LLIDPRIHFGDT"
     gene            2911..5901
                     /locus_tag="PLZ74_01065"
     CDS             2911..5901
                     /locus_tag="PLZ74_01065"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96946.1"
                     /translation="MNGRLRGMLLIGVVLGCGAAEVRFDFESGDLQGWRILDGGFGKV
                     VSDRDREHHGGGPYTKEGRFFLSTLESADGQSPRDVFTGILESPVVVLRGPRISLRVG
                     GGKGADVYVALCSLDGRELAHARGGNSQRILERVWTVPEQVGKPVFFRVSDQSEVGWG
                     HITLDSLVCQGIIDEAATEQRFRERRREIARARGGAAADRLRVAINELAERFGARYPS
                     AVLLERLGAAEAAGDEAQLDAVAREALVARNPLMNAQPILFVTRKQYRPDHHNTETMF
                     QTGEINTDKYDTEGALKVLDAASGTVRTLFAPGPGVTVRDPDVSFDGGQILFSMRRGK
                     GDDYHIYAINSDGKGLRQLTSAPGVSDIDPIWLPDGDILFSSSREPKYCMCNRHIMCN
                     LYRMGADGANIHQIGKSTLFEGHASLLPDGRVLYDRWEYVDRNFGDAQGLWVCNPDGT
                     GHALYWGNNTTSPGAVIDARALSQSHLAIAVLGSCHDRPWGALGLIDRSKGVDGLEPV
                     LRTWPVAFTNRIRTAGQNFDSTVGLQIKYEDPYPLDDRHFLCARKTGKGEETGLYYLD
                     SHGNEVLVHVEAPGCFDPMPLAPRPAPPVHPRRRNFDDPNAPGFFYVQNAMIGTHMAG
                     VAPGTIRYLRVVESPEKRNFSVRGWFGQGEQAPGMNWHNFENKRILGTVPVEEDGSAY
                     FEVPGNTFVFFQVLDANGMMVQSMRSGTYVQPGETYGCVGCHENRVGDIPPPATRPKA
                     LRRKPDRLTGWRGAARLFCFQREVQPIFNRRCVSCHDYGNEAGKKLNLAGDRDVIFST
                     SYTDLWVQGALTCVGGGPAEIQQAYSWGSHRSKLIRKVRAGHAGVKLSPEELDRLITW
                     VDLNAPYYPYYESAYPDHPGGRSPLSQDELKRLKDLTGVAISISHGQRQRSQVSFARP
                     EESRILSGLATNSAERAEALALIRAGARRLHERPRADMEGFVASVRDQEREKKYQARL
                     AEERRVYEALRNGGKRYDPGLPRE"
     gene            6097..7362
                     /locus_tag="PLZ74_01070"
     CDS             6097..7362
                     /locus_tag="PLZ74_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR96947.1"
                     /translation="MNRTLTRRALMKLTGGAAVASASASVAAATYRRSIGANDRIRIG
                     IIGCGSRGRNAHMKGLYNHVKGQPFEIVALADPWRVAREAANGLVREWFGRDAQQFSS
                     YRQLLECRDLDAVTIASCDLHHTAHLEAAARAGKHIYCEKPLAVEFDDLVRATDAARA
                     AGTVIQIGTQIRSLPSMVGARDLFRTGIFGRISRVEECRNAGRPYWYHYLKEDVREAD
                     LDWKEFLHGLPMRPFRADLFSGWYGYYEFSRGPIPNLGSHFIDLVHFITGATFPTSCV
                     CLGRINETWLDAHEFTNPNVIQATWLYPEGFVVSSSNNLANGANNVRKLYGDKGALDF
                     TNWSKPVYDCEGAPLRDGSIRGKNEVPLTEHPDHYLDWLQCMRSGGTPNASLEAGYQH
                     AVAVIMAMKSYETGRKAVYDPVQRKITTV"
     gene            7422..8723
                     /locus_tag="PLZ74_01075"
     CDS             7422..8723
                     /locus_tag="PLZ74_01075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOR96948.1"
                     /translation="MATMRKAAKRSGSKQAALALKGGAKVWDKGFPMWPSFSEETLKT
                     ALEPLVSGKVNYWTGPVGMKFEAAFAKWLGVRNAISVTNGTAALHTAIAALGIGPGDE
                     VICPSYSFIASSFCVLQAGALPVFTDVGFDHQIDPRQIEEKITERTKGIVVVHLYGVV
                     ADMGPILKIAKKHNLKVIEDCAQCFGGLYKGEKAGTLGDVGCFSFCQSKHFTTGGEGG
                     MVVTNDDDLAWECRSFRDHGYDVKERLNLLEMEGKLMYIHKRVGFNYRMTEIQSQIGL
                     CELKRFDSWNLKNRIRNGRMLKKALEKHPLVLHAPVDTPQRRNAYWWAPFVLDTSKLT
                     CPIKEFIKAIAAEGVPVYSVLWPEMYKERAYLERNGFGVANYPFFDPKARNIDYSRVS
                     CPRARWMTDRTMCFFTHPVYTEEHMQACIDAFKKVAKAYMK"
     gene            8815..10794
                     /locus_tag="PLZ74_01080"
     CDS             8815..10794
                     /locus_tag="PLZ74_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOR96949.1"
                     /translation="MIPITWLDAWIIVGFLLSVVLVGSWAARYSGQNTESFFLSGRNM
                     PWWLLGVSMVACTFSCDTPNLVTEIVRLNGVAGNWVWWAFLLTGMLTVFVYAGLWRRS
                     GLDTDLGFYELRYSGRSAAFLRGFRAIYLGVFYNLIILGGVSLAAIKIGQILFGLSPH
                     MSLLVSMTGVAIYATLGGLTGSMWADFYQFGVAMTGAVFAAMYAVRSGMADGVTSLGE
                     LFTHPAVVPNLALFRPSSHDLSTLMTVMILPLAVQWWNVWYPGAEPGGGGFVVQRMLS
                     AKNERHAVGGTLLFNVLHYAVRPWPWILVALASMVVFPLTPQPEREAARMWLEAHAAE
                     VRGYAADKAALPDEVREAVRLKRADAAGVGSLARAFPRVDDTYLGHDIAYPGMVSRMP
                     GGWRGLIVASLIAAYMSTVATLLNWGSSYVVQDVYLRFIRSQAPPRHAVRIGRLTMLA
                     MLLVSAWIAFHMTTVKDIFHVLLQVGAGTGLLYILRWFWWRINVWSEVAAMTVSFVVA
                     LFFQFGAAPLGIESAMADAGWFRLMDFTSWKMVSGILFTTVVWVAVTYLTPPVDDRTL
                     MAFCRKIRPGGPGWKRITERIQAAGDEPAGTEPCWNVPAGLVCMLLGCLLVWAALFGV
                     GYLLYGRIALGCVMSGIAAVAAYGVWKQMGRLRLG"
     gene            10805..11875
                     /locus_tag="PLZ74_01085"
     CDS             10805..11875
                     /locus_tag="PLZ74_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR96950.1"
                     /translation="MNQVRYALVGFGGIAENRIAKEGFACDVSRFRPLKQAVLVGATD
                     VNPKRRKAAQALGLKVYRDLDAVLADPGVDAVFVATNNLTHAPLARRALEAGRPVIVE
                     KPLAPNARDARMLVKLAARKKLSLSVDHMMTHNTFNREARAQVAQGTLGKVSDACFHM
                     QFAYGFDPAEAATWRCNNRAEMGGPVGDVASHCFYMAEFILDSRIRAVRAVYMPKRMA
                     IRVEDGALIQCDLENGITCSVLVSFCDVRGGLGGTLSNLGYEIYGDQAVLRAYGTLFQ
                     LSGHPDEPFAMRLELDTFDRQRRLRVAKPVNIYRGVIEEHAASILSGRPLSGEGGLHN
                     VLICEAAHASAQRGGRTVAVKG"
     gene            11897..12076
                     /locus_tag="PLZ74_01090"
     CDS             11897..12076
                     /locus_tag="PLZ74_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96951.1"
                     /translation="MGVGFANGLITPRRGVPLAACFGRGGYGTLPLVGGAPREDTAER
                     LIRAAGAGLRRACRA"
     gene            12137..13996
                     /locus_tag="PLZ74_01095"
     CDS             12137..13996
                     /locus_tag="PLZ74_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HOR96952.1"
                     /translation="MNGENGKRLRIALIYMPFAPPEGPAIGLTQIASVLKQRFGGQVD
                     IRIHYLNLDVAALPDAAGFYGAATSPYGRMTGLADWFFRAAAFPDAEDNIEAYLGRYY
                     YDGATEATEVAEFARARRGELFAFLDELIDRYALDSADLVGFSLCFFQTVASLAMASR
                     LKLRNPEVTVVFGGPAVKGVPGKTLVEHAPCVDAVFSGPGLVSFPELVGRRLAGLPIT
                     RPAIPGVFARGVADALPPACNVGAQLDIRKDVPLDYRSFLDHFDAVLGDSGRQPFLLM
                     QTSRGCWWADRRRCTFCGLNDLQERFEALPPEAALRHIRSVLGYAGRVSRFVACDNVV
                     PPNYFREVFPHLAPPQGVFIKYETRSAITAEEIRVLCRAGIRCVQPGVEALSTESLTL
                     MRKGVTAFDNLRFLKDCLRHALYVEWNLLLFSPGEREEVYRKYERDIPRLVHLQPPEG
                     VFPIEFVRDSRYFEEAGAYGLDLEPHESLRYIYPFAPETLFGLCYRFTDRNADPVWAE
                     QWLDRLGGLIETWQERWACGPEERPFLLLYRDAQSACIHDSRSGGTGRYRISDLSERL
                     LQEMGETPRTISEAAVAAEVEENAVRREIGFLNERQLLFEEDGRFLSLVISPT"
     gene            14143..14916
                     /locus_tag="PLZ74_01100"
     CDS             14143..14916
                     /locus_tag="PLZ74_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOR96953.1"
                     /translation="MNGPLFFSTFILIFLAELGDKTQLAVMSQSAASSSKWTVFAAGV
                     AALVASTAIGVLAGGLIRRFVPDERYIKCAGGLLFLLFGVLMLREVFGTKTVPVRTAA
                     PPVSAFSDWIGRFVIQQAAVFEKAAFEDYEALAARSLDPEEKALYQRLAQEERWHHQA
                     MLGAMEAGAGRDIPITAEMAGALPPFNEMMHDAVKAPRELEHAIEHERAMSRFYQILA
                     EKSTLPRLRETFRALAAAEANHATRLLERMRNAETKTTE"
     gene            14932..15255
                     /locus_tag="PLZ74_01105"
     CDS             14932..15255
                     /locus_tag="PLZ74_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOR96954.1"
                     /translation="MDEWVDIRKDERHVARERAKARVLRNSAWWREQIRPGVCRYCGK
                     TVGADALTLDHVVPVARGGTSTRGNVVPACVACNREKNVLTPAERILTELERDAAAKT
                     GHDDV"
     gene            15248..16195
                     /gene="ispH"
                     /locus_tag="PLZ74_01110"
     CDS             15248..16195
                     /gene="ispH"
                     /locus_tag="PLZ74_01110"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOR96955.1"
                     /translation="MCNRRKLTVVTPHGFCSGVARAVATAEAVLAACPGERVYCLNEI
                     VHNRQVVSRLTDAGMVFVSDPAEVPQGARLLFSAHGVSPEVRRQAAARGARVIDATCP
                     FVMKVHHEVVRFAAQGACVVCIGHRHHEEVVGVAGEAPDQVRIVECEAEAESLDVPST
                     QPVAVVTQTTLDAARVEGVMRVLRRRFGARLIVPASTDVCYATRNRQRAVSLLAERCG
                     LVLVLGSENSSNSQRLVETARTAGARACLVSTPSCLAEVAGLADEPAVGLTSGASTPE
                     AFLDEVIRLLCEGYGFPAPEILCAVEEESHVFRLPPLPE"
     gene            16316..16735
                     /locus_tag="PLZ74_01115"
     CDS             16316..16735
                     /locus_tag="PLZ74_01115"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOR96956.1"
                     /translation="MTDPECVFCRIIAGQIPAKVLFENEEAIAFLDIAPFEPGHALVV
                     PKMHAALFTELPDSVLSHLMPLVKRVASLLLERLPCDGFNILQNNGRCATQVVPHVHF
                     HVVPRWNGRAINWISGAYASAEEMESVYRKLTVAAGV"
     gene            16732..17166
                     /locus_tag="PLZ74_01120"
     CDS             16732..17166
                     /locus_tag="PLZ74_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96957.1"
                     /translation="MKGRDISFREVVQGICAKDPRYDEDAYLFLMEALDSTVKAIREK
                     EPEHGRDVSGRELLEGIRAFALDEYGPLAYTVFAEWGIHETADFGAIVFNLVEAGRLG
                     KSESDSLEDFNQVYDFDEAFRLPFEPQEPPEKDGAAGKRQRG"
     gene            complement(17194..17850)
                     /gene="lipB"
                     /locus_tag="PLZ74_01125"
     CDS             complement(17194..17850)
                     /gene="lipB"
                     /locus_tag="PLZ74_01125"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOR96958.1"
                     /translation="MRELEVSDLGRDAGYNEVLALQERLHTRRAAREIPDTLLLLEHR
                     PVYTLGRSAAAAHVLYSEERLKEAGIERVQTSRGGDVTYHGPGQLVGYPILHLGDAGL
                     RVLEYVTALEETLIRAVADFGIVAARDPRNRGVWVGNAKLAALGIRVAHQVTMHGFAL
                     NVAPRLDDYRGIVACGLTDASVTSLQHLLGEAPDMPAVKQSVVRAFREVFGYDRNITA
                     "
     gene            complement(17938..18201)
                     /gene="rpsT"
                     /locus_tag="PLZ74_01130"
     CDS             complement(17938..18201)
                     /gene="rpsT"
                     /locus_tag="PLZ74_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOR96959.1"
                     /translation="MANIKSAIKRAKQSEARNVRNRAVKSRVLTARKKVLAAISAGEK
                     EAAQKLYSDYTSVLDKAAKKGVIPKNTASRKKSRVALSMQKMA"
     gene            complement(18422..19780)
                     /locus_tag="PLZ74_01135"
     CDS             complement(18422..19780)
                     /locus_tag="PLZ74_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96960.1"
                     /translation="MNRKRDALISMTVAMGVAACCFAEFESGCPEYAPELKDRCWMWG
                     HKTGQYDQPNGWFRIPVSPDVTMAEACRYMGIPNVSIVIWHIPDEKFMSEFTDMKRVA
                     WVVDNGDLHLHGRDILPEFLAFGEKKMKTMPNLMGFEFDDFFDQPRKPVRKEKLADGT
                     EVNVLPGSRTLEELRALRKRLHSYDRPIDMRLVFYERDLDHPEETKAVVDIFDTVMFW
                     TWFGKNVQALENNFKAYRKIAPKKPTLLGIYMWDFGGSQPLDATSMKLQLDFALKLFR
                     AGEVEGFIFHCTPLVNKNIEAVELARRWIIEHGNEKHGGGEQRVPPIPEPPALRPEQT
                     MTDFGRLAFEEGKKPNGALLLTGPGKIRIVERTGRIAWENVAQANPEKAFYHGGHVYF
                     EEDGIQKRIRYSLPCDRLAKAEVVPEAQRAQVPKNKKFDSALLDCLKDRSGYSGAMNL
                     QR"
     gene            19978..21060
                     /locus_tag="PLZ74_01140"
     CDS             19978..21060
                     /locus_tag="PLZ74_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOR96961.1"
                     /translation="MRHAYAVILAGGSGERFWPLSTKARPKQFISLFGGRPLLALAVE
                     RLSGLIPADRILIITAEHLVEQTVEAAWHVPHANIIGEPCKRDTAPAVAVACGLVRQR
                     DPEGVVCILTADQLMSDVETFRVTLADAIRVAGCEEAIVTIGIKPDTPATGFGYIEVG
                     EAVETRTVTLFNRAKRFVEKPDAYHAARYVESGTYFWNAGMFIWKCDVMREALERFAP
                     DLAELCDAVVAAAPGPELANLLQRVYPSLRAISIDYAVMEHAPNILVARGEFGWDDVG
                     SWPAVAGHFSADLAGNVVVGSCEPMESLNNIVVSENRLTALIGVSNLVVVQSDNATLI
                     CPQDRAEDVKKLLRRIAQRPDGEKYV"
     gene            21088..21897
                     /gene="truA"
                     /locus_tag="PLZ74_01145"
     CDS             21088..21897
                     /gene="truA"
                     /locus_tag="PLZ74_01145"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOR96962.1"
                     /translation="MAANGQRKPGGGPTAATLPGPVARRFRLTVAYDGTAYAGWQVQP
                     HHPSVQQTLEEALAGIVGHTVKVHGSGRTDQGVHARGQVAHVDLVCRMTPVAVGRALN
                     ARLPQDIRIRSVARVRPDFHARRSAVGKEYRYFVWNGAQLLPDRRLYAAHVFRPLDVE
                     AMRQAAAFFAGQHDFASFTANPQREVPSTVRRVFDVTVGKRGREIVFRVRGEGFLYKQ
                     VRSMVGFLLRVGEGAEPPEAVRDLLASRAPRKARVPSAPARGLFLWRVWYA"
     gene            21951..24293
                     /locus_tag="PLZ74_01150"
     CDS             21951..24293
                     /locus_tag="PLZ74_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOR96963.1"
                     /translation="MGEGRERMRVSANRKPGELIAALQAVQDACGYVPPEELARIAVT
                     MGVSEAELTGVVTFYDQFRLRPVGRHRIRVCVGTACHVKGAERTVEAFRRALRIPEGS
                     DTDAEGEFTVERVACLGCCMLAVAVQVDGVIYGQVTPARVKAVLRDVRAGETQIRDTA
                     DVPHPQTGARVRLCACSSCAAAGAADVLHEIRRVVRTWRLPVGVREVGCTGGSYHAPL
                     MDILLEDGLHFRYGRVRVRDVERILLSHIRPARTAARLQATLRAWTEQLFAGEEDDPP
                     VRFPLSVRDQSDAGYWLCQHRIATEGAGERSSLDWDEYCRHGGFEAYRTCARQMTPQE
                     VVAQLEASGLRGRGGGGFGTGRKWRAVADAAQGSDAVVVCNGDEGDPGAFMDRMLLES
                     YPFRVIEGMMIAAHAVGASEGIFYIRHEYPLAVRRVREALALLEREGILGAEVPLRLR
                     VVEGAGAFVCGEETALLSSVEGRRGVPHVRPPYPSESGLFGRPTLVNNVETLALVPWI
                     VRHGAAAFRAVGTAGSPGTKAFALAGKVARGGLVEVPMGLPLRQVVEQIGGGVGEGHR
                     FKAVQVGGPSGGCIPADLSDMPVDYETLTRAGGMMGSGGMVVLDETDCMVDIARYFLS
                     FTQRESCGTCTGCRIGTRRMLEILERLCNGEGRPEDPAALERLAESVRAGSLCGLGRT
                     APNPVLSTLRHFRAEVEAHVRGVCPARACRALIAYTVSDRCIGCTLCAQNCPAEAIAF
                     TPLERAVILQDRCTKCGSCRQVCPEKAIEIEDAKRDNEIR"
     gene            24307..24783
                     /gene="queF"
                     /locus_tag="PLZ74_01155"
     CDS             24307..24783
                     /gene="queF"
                     /locus_tag="PLZ74_01155"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOR96964.1"
                     /translation="MRRTKKTVPGVTRLGSQGTVYADQYDPSVLEAFANQHRDRDYWV
                     TFTCPEFTSLCPVTGQPDFGTIIIRYVPDQRMVESKSLKLYLFSFRNHGDFHEDCVNV
                     ILNDLKALMDPKYIEVWGRFAPRGGIAIEPYVNFGRPDSAFAALAETRFATHPAAR"
     gene            24842..26074
                     /locus_tag="PLZ74_01160"
     CDS             24842..26074
                     /locus_tag="PLZ74_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOR96965.1"
                     /translation="MTRQIGFTDTVVRGVRVLLLAALWCAGVGARAAEPTTAPFAGKI
                     RLLLPPRLYAVPGIEMNVYFDNVCLMPNPESVVFDVTCTKGRQQVERWTFVPEEEDAG
                     EYPFVLEVRDAANTIIARAESRLRVTPRAAGAGAPVTVLTVGDSLTHATVYPAHLLEL
                     CKAEGNPHVTLVGHTPYTNRPAIRIEGYGGWTAKQFTTGYKEGKRDTGSQDWRVWNAS
                     SSPFLYADAQGVPALDFARYCREFNGGRAPDFVTFLLGCNDLFGADDGSIDAILDAMF
                     KHYDALIEMVRGVSRETRIGVLLVTPPAGTQDAFGANYRCGLTRWQFKRNQHRTVERM
                     MERYGGREGENLFLVPVHVNLDCVHNYPTLQGPWNARTREAGSRLNNGVHPSREGYLQ
                     IGDSLFCWIKAQLVADNF"
     gene            26392..27060
                     /gene="queC"
                     /locus_tag="PLZ74_01165"
     CDS             26392..27060
                     /gene="queC"
                     /locus_tag="PLZ74_01165"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOR96966.1"
                     /translation="MRKNKAVVIFSGGQDSTTCLAQAIRDYGEGNVSCITFQYGQKHS
                     REVEVAKEIARIFRISDHPILALDWYRQVTTNALLDSDMAIAAGQGGACPNTVVDGRN
                     MLFLLVAAIYAKSRNIRDLIIGVGETDYSGYPDCRDVFVKSCNETLNLAMAYPFRVIT
                     PLMHMTKAETWALADKLGILELVAEKTLTCYNGVVGRGCGTCPGCLLRQRGYEAYLAR
                     RAHG"
     gene            27113..27523
                     /gene="queD"
                     /locus_tag="PLZ74_01170"
     CDS             27113..27523
                     /gene="queD"
                     /locus_tag="PLZ74_01170"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOR96967.1"
                     /translation="MLTVSKQCQFDAAHVLPHHAGQCKNLHGHTYRVIVEVAEDADSE
                     DMVIDFKDLKQVIRDVIVDRFDHAFLYDETSESECEIAAVIAKHGMRSVGLPFRSTAE
                     NLARYFFHELATRVNVVSVKVYETPESCAEYRRG"
     gene            27530..28162
                     /locus_tag="PLZ74_01175"
     CDS             27530..28162
                     /locus_tag="PLZ74_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOR96968.1"
                     /translation="MCERQSYPVVEIFESLQGEGYNTGLESVFLRFGGCNLKCPWCDT
                     RHETFETLDEETVVARVTACSARAVILTGGEPFLQTGLEGLLTRFRDAGFWIGAETNG
                     VLAPSAEVLRRVDYLTVSPKALYAPLYDDARMVRRADEVRIVVDGEVRDFCMAMRNRI
                     EAPRYYLSPCERDGTFNIGETVRLLGELNRGRRSGKWRLSLQTHKLAGFR"
     gene            complement(28201..28276)
                     /locus_tag="PLZ74_01180"
     tRNA            complement(28201..28276)
                     /locus_tag="PLZ74_01180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28241..28243),aa:Lys,seq:ctt)
     gene            complement(28287..28362)
                     /locus_tag="PLZ74_01185"
     tRNA            complement(28287..28362)
                     /locus_tag="PLZ74_01185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28327..28329),aa:Gly,seq:gcc)
     gene            complement(28517..29212)
                     /locus_tag="PLZ74_01190"
     CDS             complement(28517..29212)
                     /locus_tag="PLZ74_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOR96969.1"
                     /translation="MLQPNRRMTLQAVLFDLDGTLVDSERLWAEAMRDLLVDRQCPCD
                     TETVLRIVYGHSWIDIYQALVTRFPVLSEIPRHRLAEHLRVYYERLRGQGEGVVIRSS
                     VTLLKQLARDYPVILVSGSPRHDLEEAVRLMEIERDVRFVLGAEDYEPGKPSPAGFLL
                     GARRLGVPPEHCLVFEDSRVGVLAAKRAGMLCVALARPGSHPQELSEADWILEDLAAF
                     TVDGLRKRLARPK"
     gene            complement(29188..29688)
                     /locus_tag="PLZ74_01195"
     CDS             complement(29188..29688)
                     /locus_tag="PLZ74_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HOR96970.1"
                     /translation="MSDLCPCGSGIEFESCCEPFLLERQKPLTAQALMRSRYCAYAMG
                     DIDYLYQTSGAKVRKEFDAESSRNWAKNAKWTGMEIVSLEGGGENDDTGVVEFIAHYS
                     VKETDFNHHERAEFGRVGGEWRFLDGKIFGPDPVRRDHPKIGRNDPCPCGSGKKYKKC
                     CSQTVA"
BASE COUNT         5402 a         8410 c        10271 g         5778 t
ORIGIN      
        1 cgcgcaaccc ttcgccgatc agcaccgcca gcagcatcac cgtgaacagg gccagtgacg
       61 ggaagagaat cagccaccag gcccagcgga actgttgcgc ctgctgcaag agttcgcccc
      121 aactcggcgt gagcggcggc aagccgaagc ccagaaaatc cagcgcggcg agcgaaccga
      181 tggcgccgat cagcgtaaag gggaaaagcg tgatcagcgg cgtcaaggcg ttcggcagga
      241 tgtggttgaa aatgatgcgg gccgacgaga gcccttgaca gcgcgcggcc tccacaaagg
      301 tgcggttgcg cagacgcaga aattcggcac gcaggtagta ggagatgccg atccagttaa
      361 acaggccgta acagagcagg agcagcgaaa aactgcggcc cagagccgag ccgatgaaaa
      421 tcatcacgta cagaaagggc aacgcgctcc agatctcggt cagccgctgg cccaggatat
      481 cgacccatcc gccgaaataa ccttgggctg cgccgatcac cacaccgaac aatacggccc
      541 agagcgccaa cagcaggccg aaaagcatag ccgtgcgcat tccgtacaac acgcgcacaa
      601 ggacatcccg tccggccgag tcgatcccca tccaatgccc ccggaccggc gggaagggcc
      661 acgccacctc cgtgagcccg caggccacgg tcgcgtggcg gtccttccag gggatgcggg
      721 ccgtgggcgc ggtaccggcg aacgcggcgg aagcgagagc gaggatttcg ggttggcacg
      781 ttgcggggat ctgacgccac gtgcggtgcg ttacgggata gaccgttccg gcgtcatcgc
      841 gccggaaccg cacttgtacg ggcaggtcgg gcgtttcgat ctggcggaac atgaggcggg
      901 gcgggcctgc ggccccggcg gtttcgggca gcgtcacgcg cgcgcgcaaa tcggattgcc
      961 ggagatgcgt gagcaggaag cgttgcgcgg gcgcggggcg gcccgcggcc cgtgcgtcca
     1021 gcgacgcgcg cagggtttcc ggcacatgcc agtgccggtc gagcaccacg ccgtttacac
     1081 gcgcctcgcc ggggaagaag ggttcgcagc cttccgcacg gatcaccgtg ccgtccggca
     1141 gcaggttgag gcgacccacg cggatctcgg gcacgaccga aacatggaca gtgcggaaac
     1201 ggctgagcgt cccggcgtcg accacgtcac cggggctgta cggcaccggc gcccagacca
     1261 cgcggttgag cgggttggtg gtgaaggcgg gtgatcgaac gaacgcgcgg aagttcacgc
     1321 gggtcgcctc cgcatcaggg ccttccagat cgcgcagtgt caggcgttgc aggaacggca
     1381 gccaggtgcg gccgttcacg cgcagaaaga gggggcgccc gttgcagagc cattccgcgc
     1441 acagactcac cgcgaacagg gcggtcagca atatgagcgc cacgcgtgcg cggcggatgc
     1501 gccaaaagcg ctccagccgt ttacgtgtca gcggcgagag ccggtagcgg ggaaatcgcc
     1561 tcatgtgtca ccaaagtgga ttctcggatc gatcaacagg tagcagaagt cggaaagcag
     1621 gttccccgcg agttgcagca gggaggtcaa cgcgagcagc gccagaaaca ccgcatagtc
     1681 acggccggtg agcgcctccc agctcagtcg gcccatgccg ggaatctcga agatgttttc
     1741 gatgatgatc gaacccgcga acagcacccc gaggatcgac ccgaaaccgg tcgcgaccgg
     1801 aatgagcgcg ttgcggaacg cgtgccccca gatagcgcgg cggcgggtcg cgcctttcgc
     1861 gagcacggtg cgcacgtaat cgctggcgat ctgatcgagc agcgcgtttt tcatgatcag
     1921 cgtgagcacc gcgaaatttc ccgctacata gcaggtcacc ggcaacgcca tgtgccaggc
     1981 gcggtccgcc attcgggcac cccaacccgc agtggccggc aggtcggatt cgaacccgcc
     2041 gagcgggaag aggtcggcca gcccctcgac cgtgccgcag aagaacattt tgagcaccat
     2101 gccgagggcg aaggcgggga tggcatatcc cgcgaacacg atcaggctcg agatggcgtc
     2161 gaagggctgg cggtgccgca gggctttggc caccccgagc gggatgcaga tcaggtagct
     2221 cagcacgaat ccggtgatgc cgaaccagag cgagaccggg atccgggagg cgatcagttg
     2281 ccaggcggtc cggttggggt aatcgtagga ggcgatgagc aatcccatcc gtttgcgcac
     2341 gagccagtcg taatattgca ccggcagggg gcggtcgaat ccgaactggc gggtcaattc
     2401 ctggcggtac tcttcggtga cctgcccgac cgcggcactg cccggatcat tcgccccgcc
     2461 gagattccgc atcttcatca gctggatttc gaccggaccg ccgggcaaaa agcgcgtcag
     2521 cgcaaaacac accatcgtga tccccagaaa ggtgggaatc accaacagca ggcgccggac
     2581 cagataggcg aaacgattca tagtggaaca agcccgccgc agaatacgcg gaatcacagg
     2641 caacggtccg gagtgtagta aaaaaaaagg cgcgagacaa tacggaggta atcgactggt
     2701 ggcaagggga tgctgttttg tcgcatgaaa accgtgcccg ccccggcgtc cctgttcagc
     2761 gttccggtta tcccgcttac ggaagattgg ccttgcgccg ggttgagtcc tgtgacaaaa
     2821 ttttcctgtt cggtgaatgc cggacacgga attcgcgcct attgttcggc atcgggcaag
     2881 gcggtcctta ccggagggaa gggaacagtg atgaatgggc ggttgagggg gatgcttttg
     2941 attggcgtcg tattggggtg cggcgctgcg gaagtacggt tcgatttcga gagcggggat
     3001 ctgcaaggct ggcgtattct ggacggcggg ttcgggaagg tggtaagtga tcgggaccgc
     3061 gagcatcacg gaggcggacc gtacaccaag gaaggccgtt ttttcctctc gactctggaa
     3121 agcgcggacg gacagagccc acgtgacgtg tttacgggca ttttggagtc accggtggtg
     3181 gtgttgcgtg gcccgcggat ttcgttgcgg gtcgggggcg gaaaaggcgc ggacgtgtac
     3241 gtggcgttgt gctccttgga cgggcgggag ttggcgcatg cgcgcggcgg aaacagccag
     3301 cggatactcg aacgtgtctg gacagtgccg gagcaggtgg gcaagccggt ttttttccgg
     3361 gtgtcggacc agtcggaagt cggctggggg cacatcacac tggacagtct ggtctgccag
     3421 gggatcatcg acgaggcggc cacggagcaa cggttccggg aacgcaggcg tgagatcgcg
     3481 cgggccaggg gcggagccgc cgcggaccgg ttgcgcgtgg cgatcaacga attggcggag
     3541 cgtttcggcg cgcgttaccc ctccgctgtc ttgctggaac ggttgggtgc ggcggaggcc
     3601 gcgggagatg aggcccaact tgacgctgtg gcgcgggaag cgctggtggc gcgcaacccg
     3661 ctgatgaacg cgcagccgat cctcttcgtg acgcgcaaac aatatcgacc ggaccaccac
     3721 aacacggaga cgatgttcca gaccggggag atcaacacgg acaaatacga caccgagggc
     3781 gcgctcaagg tgttggacgc cgcctccggc acggttcgga cgcttttcgc accggggccg
     3841 ggtgtgacgg tgcgcgatcc ggacgtgagc ttcgacggtg gccaaatcct cttttcgatg
     3901 cggcgcggga agggggacga ttaccatatc tacgcgatca acagcgacgg caagggactg
     3961 cggcagttga cctcggcgcc gggcgtatcg gacatcgacc cgatctggct gccggacggg
     4021 gatattctct tctccagttc ccgcgagccc aagtactgca tgtgcaaccg ccacattatg
     4081 tgcaatctct accgtatggg cgcggacggc gcgaacatcc accagatcgg gaaatccacg
     4141 ctctttgagg ggcacgcctc gctgttgccg gatggacgtg tgctgtatga ccgttgggag
     4201 tatgtggacc gcaacttcgg tgacgcgcag ggtttatggg tgtgcaatcc ggacggcacg
     4261 ggccatgcgc tctactgggg caacaacacc accagcccgg gtgccgtgat cgatgcacgg
     4321 gcgctctcgc agagtcattt ggcgattgcc gtgctgggct cctgccatga ccgtccctgg
     4381 ggggcgttgg gtctgatcga ccggtcgaag ggcgtggatg ggttggaacc cgtattgcgc
     4441 acctggccgg tcgctttcac aaaccgcatc cggaccgcag ggcagaattt tgattcgacg
     4501 gtagggctcc aaatcaaata cgaggatcca tacccgttag atgaccgcca tttcctgtgc
     4561 gcgcgcaaaa cgggcaaagg ggaggagacg gggctgtact atctggattc gcacggtaac
     4621 gaggtgttgg tccatgtcga ggcgccgggg tgtttcgacc cgatgccgct ggcaccgcgg
     4681 cccgcaccgc cggttcaccc gcgccgccgg aatttcgacg acccgaacgc gcccggtttt
     4741 ttttacgtgc agaatgcgat gatcggtacg catatggcag gggtggcacc gggcacgatt
     4801 cgctatctgc gcgtggtgga atcaccggag aaacgcaact tttcggtacg cggatggttc
     4861 ggtcaaggtg agcaggcacc gggcatgaac tggcataatt tcgaaaacaa gcggattctc
     4921 ggcacggttc cggtggagga ggacggcagc gcctatttcg aggttccggg caacacgttt
     4981 gtgtttttcc aagtgttgga cgccaacggc atgatggtgc agtccatgcg ttccggaacc
     5041 tatgtgcagc cgggcgagac ctacgggtgc gtgggttgtc atgagaatcg cgtgggcgac
     5101 atcccgccgc ctgcgacacg gcccaaggcg ttgcggcgca aaccggatcg cctgaccggc
     5161 tggcgcggcg cggcgcggct cttctgtttc caacgcgagg tgcagccgat tttcaacagg
     5221 cggtgcgtct cctgtcacga ctacggaaac gaggcgggca agaagcttaa tctggcgggg
     5281 gatcgcgatg tgatcttcag cacctcgtac acggacttgt gggttcaggg tgccttgacc
     5341 tgcgtgggcg gcggaccggc cgagatccag caggcataca gttggggtag ccatcggtcc
     5401 aagttgatcc ggaaggtccg tgccggccac gcgggggtca agttatcacc cgaggagttg
     5461 gaccggctga tcacatgggt ggacctcaat gcgccgtact atccgtatta tgagagcgcg
     5521 tatcccgatc atcccggcgg acggagtccg ttgagtcaag acgagttgaa gcggctgaag
     5581 gacctcaccg gcgtggcgat ttccatctcg catggccagc ggcagcggtc gcaggtcagt
     5641 ttcgcccggc cggaagagag ccggattctg tcggggctgg cgacgaattc ggcggaacgt
     5701 gccgaggcgc tggcgctgat tcgcgcgggt gcgcggcggc tgcacgaacg gccgcgcgcg
     5761 gacatggagg ggtttgtggc gtccgtccgg gatcaggaac gcgaaaagaa atatcaggcg
     5821 cggttggctg aagagcggcg cgtgtacgaa gcgttgcgga acggcgggaa acgttacgat
     5881 cccggcctgc cgcgcgaatg aattcccgtt ccgtccggat tttcgcgtat attctggaaa
     5941 aaggtttaca gcaccggaaa cggaacttgt cgggccggaa gggttgccgt atactgggcg
     6001 acaataaagg aatttcggta tggcacgcgg gggttggctc cgcgacgttt ccggtcggca
     6061 tccggaacgt gagctgaaac cgggagggga acgtttatga accgtacatt gacccgtcgc
     6121 gcactcatga aactcacagg aggcgcggct gtcgcatcgg cttccgcatc ggtggcggct
     6181 gccacgtacc gccgttcgat cggcgcgaac gaccgcatcc gcatcgggat catcggctgc
     6241 ggttcgcgcg gacgcaacgc gcacatgaag gggctttaca atcatgtgaa ggggcagccc
     6301 ttcgagatcg tggcgctggc agatccttgg cgcgtggccc gcgaagcggc caacggtctg
     6361 gttcgggagt ggttcggacg tgacgcgcaa cagttcagct cctatcgtca gctgctcgaa
     6421 tgccgggatc tggacgcggt caccatcgcc tcctgcgatc tgcatcacac cgcgcatctg
     6481 gaggcggcgg cacgggcggg caaacatatt tactgcgaga aaccgctggc ggtggagttc
     6541 gatgatctgg tccgtgcgac tgacgcggcc cgggccgcag gcacggtgat ccagatcggc
     6601 acgcagatcc gcagtcttcc ctcgatggtg ggggcgcgcg atcttttccg gaccgggatc
     6661 ttcggcagga tctcgcgtgt ggaagagtgt cgcaacgccg ggcgtccgta ttggtatcat
     6721 tatctcaaag aggatgtccg ggaggcggat ctggactgga aagagtttct gcacgggttg
     6781 ccgatgcggc cgttccgcgc cgatctcttc tccggctggt acggctatta cgagttctcg
     6841 cgcggtccga ttccgaatct cggctcgcac tttatcgatc tcgtgcattt cattaccggg
     6901 gcgaccttcc ccacctcgtg cgtctgtctc gggaggatca acgagacctg gctggacgct
     6961 cacgaattca ccaatccaaa tgtgattcag gcaacttggc tctatccgga agggttcgtg
     7021 gtcagctcgt ccaacaacct tgccaacggc gcgaacaacg tccggaaact gtatggcgac
     7081 aagggcgcgc tcgatttcac gaattggagc aagccggtct acgactgcga aggggcgccg
     7141 ttgcgtgacg gctccatccg cgggaagaac gaggttcctc ttacggagca ccccgaccat
     7201 tatctcgact ggctgcaatg catgcgcagt ggcggaacgc ccaacgcttc gctggaggcc
     7261 ggctaccagc acgccgtggc ggtcatcatg gcgatgaaat cctatgaaac cgggcgcaaa
     7321 gcggtttacg atcctgtcca acgcaagatc acgacggtgt gagagaggcg ggcaaatcgg
     7381 gcaggggagg acagcggaac ccgaaccgaa gggagaagca catggcgacc atgaggaaag
     7441 cagcgaaacg gagtggttca aaacaggcgg cgctggccct taagggcggc gcgaaagtat
     7501 gggataaggg gttcccgatg tggccctctt tttcggaaga aaccctgaag acggcgctgg
     7561 aaccgctggt gagcggcaag gtgaactact ggaccgggcc ggtcggcatg aagttcgagg
     7621 ccgcgttcgc caaatggctg ggagtgcgca atgccatcag cgtgaccaac ggcaccgcgg
     7681 cattgcacac cgcgatcgcc gcgttgggta tcgggcccgg ggacgaggtg atctgtccgt
     7741 cttactcgtt catcgcgtcg tcgttctgtg tcctgcaggc gggtgcgctg ccggttttca
     7801 cggatgtggg gttcgaccac cagattgatc cgcggcagat cgaggagaag atcaccgagc
     7861 ggaccaaagg catcgtggtg gtgcatctct acggcgtggt ggcggacatg gggccgatcc
     7921 tgaagatcgc caagaaacac aacctgaagg tgatcgagga ctgcgcccaa tgtttcggcg
     7981 gcctgtacaa gggggagaag gcgggcaccc tcggggatgt cggctgtttc agtttctgcc
     8041 agagcaagca cttcaccact ggcggcgagg gcgggatggt ggtcacgaat gacgacgacc
     8101 tcgcctggga gtgccgttcg ttccgcgatc acggctatga cgtgaaggaa cgcctgaacc
     8161 tgttggagat ggaaggcaag ctgatgtata tccacaagcg ggtcggcttt aactaccgca
     8221 tgacggagat ccagtcgcag atcggactgt gcgagctgaa gcgttttgat tcttggaatc
     8281 tcaagaatcg gatccgcaac ggcaggatgt tgaaaaaagc gttggagaag catccgctcg
     8341 tgctccatgc gccagtagac accccgcagc gccggaatgc gtactggtgg gcaccgtttg
     8401 tgctcgacac gtcgaaactg acctgcccga tcaaggagtt catcaaggcg atcgcggcgg
     8461 agggggtgcc cgtctacagc gtgctctggc cggagatgta caaggaacgg gcctacctcg
     8521 aacggaacgg attcggcgtc gccaactatc cgttcttcga tcccaaagcg cgtaacatcg
     8581 actacagccg ggtgtcgtgc ccgcgcgcgc ggtggatgac cgaccgcacg atgtgtttct
     8641 tcacccatcc ggtttatacc gaagagcaca tgcaggcctg tatcgatgct ttcaagaaag
     8701 tggcgaaggc atacatgaaa tagggatcgg gagacgggaa acgggaagtg tgggacggga
     8761 aatgtggcat cgacgccaca gccccgtttt cccgtttctc ctttggagtg ccgtatgata
     8821 cccatcactt ggctggacgc gtggatcatc gtggggtttc tgttgtcggt cgtgctggtc
     8881 ggttcttggg ccgcccggta ctctggccag aacaccgaat cgttttttct ttcggggcgg
     8941 aacatgcctt ggtggctgtt aggggtctcg atggtggcct gcaccttttc gtgcgacacg
     9001 ccgaacctcg tcacggaaat cgtgcggttg aacggcgtgg cgggcaattg ggtgtggtgg
     9061 gcgtttctgc tgaccggcat gctgacggtt ttcgtgtacg ccggtttgtg gcgacgctcg
     9121 gggctggaca cggatctggg tttttacgaa ttgcggtaca gtggccggtc ggccgcgttt
     9181 ctgcgcgggt tccgcgcgat ctatctgggc gtcttctata acctcatcat tttgggcggc
     9241 gtgtcgctgg cggccatcaa aataggacag attctgttcg ggttgtcgcc gcatatgtcg
     9301 ctgctggttt ccatgaccgg ggttgcgatc tatgcgacct tgggtgggct gacgggttcg
     9361 atgtgggcgg atttttatca gttcggcgtg gcgatgaccg gtgccgtgtt cgcggcgatg
     9421 tatgcggtcc gttccggcat ggcggacggc gtcacgtccc tcggagaact gttcacgcat
     9481 cctgcggtgg ttcccaatct cgcgttgttc cggccgtcgt cgcatgatct ctcgaccctg
     9541 atgaccgtga tgattctgcc gctggcggtg cagtggtgga atgtgtggta tccgggggcc
     9601 gagccgggcg gaggcggttt cgtggtgcag cggatgcttt ccgcgaaaaa cgagcggcac
     9661 gcggtcggcg gcacgctgct cttcaatgtg ttgcactacg cggttcggcc ctggccctgg
     9721 attctcgtgg cgctcgcttc gatggtggtt ttccctctga ccccgcaacc ggaacgggag
     9781 gctgcccgga tgtggctgga ggcgcatgcg gctgaggtgc gcggatacgc tgccgacaag
     9841 gccgcgctgc cggacgaggt gcgcgaggcg gtccgcctga agcgcgcgga tgcggctggc
     9901 gtggggtcgc tggcgcgtgc tttcccgcgc gtggacgaca cctatctcgg tcacgacatc
     9961 gcctatccgg gtatggtctc gcgcatgccc ggcggttggc gggggctgat tgtggcgtcg
    10021 ctgatcgcag cctacatgtc taccgtggcc acgctgttga attggggctc gtcgtatgtg
    10081 gtgcaggatg tttacctgcg cttcatccgc tcgcaggccc cgccgcgcca tgcggtacgc
    10141 atcggccggc tgaccatgct ggcgatgctg ttggtttcag cctggatcgc gtttcacatg
    10201 accacggtca aagacatctt ccacgtactg ttgcaggtgg gcgcgggcac gggcctgctc
    10261 tacatcctgc gctggttctg gtggcggatc aacgtgtgga gcgaggtggc cgccatgacg
    10321 gtctcttttg tggtggccct gtttttccag ttcggggccg cgccgttagg catcgaatcc
    10381 gcaatggccg atgccggatg gttccgcttg atggatttca cctcatggaa gatggtgtcg
    10441 ggaattctgt tcaccacggt ggtatgggtc gcggtgacct atctgacccc gccggttgac
    10501 gaccggacgc tgatggcctt ctgccggaaa atccgtcccg gcggtccggg gtggaagcgg
    10561 atcacagagc ggattcaagc ggcgggggat gagcctgcgg gaacggaacc gtgttggaat
    10621 gtgccggccg gtctggtgtg tatgctgctg ggctgcctgc tggtgtgggc cgcgcttttc
    10681 ggcgttggtt atctgctgta tggccggatc gcactggggt gtgtcatgtc gggcatcgcc
    10741 gcggttgcgg cgtacggggt ttggaaacag atgggccggt tacggttagg atgaggggta
    10801 cgcgatgaat caagtgcggt atgcgttggt ggggttcggc gggattgcgg agaaccggat
    10861 cgcgaaagag gggtttgcgt gtgacgtgtc ccggttccgg ccattgaagc aggcggtgtt
    10921 ggtcggggcc acggatgtga acccgaaacg gcgtaaggct gcacaagccc tgggattgaa
    10981 ggtgtatcgc gatctggatg ccgttttggc ggatccgggg gtggacgcgg tttttgtggc
    11041 gacgaacaat ctgacgcatg cgccgctggc gcggcgggcg ttggaagcgg gccgtccggt
    11101 gattgtcgaa aagccgttgg cgccgaacgc gcgcgatgcc cggatgctgg tcaagctggc
    11161 ggcccgcaaa aagctcagcc tctcggtcga ccacatgatg acccacaaca cattcaaccg
    11221 ggaggcacgc gcacaggtgg cccaaggcac tctgggcaag gtgagcgacg cctgtttcca
    11281 catgcagttc gcgtacggat tcgatccggc ggaggcggct acttggcgtt gtaacaaccg
    11341 ggcggagatg ggtggcccgg tcggggatgt ggcaagtcac tgtttttata tggccgagtt
    11401 cattttggac agccggattc gggcggtccg tgcggtgtac atgcccaagc gcatggccat
    11461 ccgggtggag gacggtgcgc tgatccagtg cgatttggag aacgggatca cctgttccgt
    11521 gctggtctcg ttttgtgacg tgcgcggtgg tttgggcggg acgctttcaa atctcgggta
    11581 tgagatttac ggcgatcagg cggtgttgcg cgcctatggc acgctgttcc agctttccgg
    11641 gcatccggat gaaccgttcg cgatgcggct ggagttggat acctttgatc ggcaacggcg
    11701 tctccgggtc gcgaaaccgg tcaacatcta ccggggggtg attgaggagc atgcggcctc
    11761 gattctgagt ggccggcctc tgagcgggga gggggggctg cacaatgtgc tgatctgcga
    11821 ggcggcccac gcatccgcgc aacgcggcgg acgcacggtg gcggtgaagg ggtgatccgg
    11881 acggaaagag ggagcgatgg gcgtcgggtt tgcgaatgga ctcatcacgc ctcgccgcgg
    11941 ggtgccgctg gccgcgtgct tcggccgcgg cgggtacggg accctgccgc tcgtgggtgg
    12001 cgcgccgcgt gaggatacgg cggagcggct gatccgcgcg gctggcgcgg gtcttcgtcg
    12061 cgcatgcagg gcgtgaccgc cgggcaaatt cttgggctcg acggtttgtc cggaacgtgg
    12121 catcctgaac cccggaatga acggggaaaa tggcaaacgc ctcaggatcg cgctgatcta
    12181 catgccgttt gcgccgccgg agggtccggc gatcggtctc acgcagatcg cgtcggtgct
    12241 gaaacagcgg ttcggcgggc aggtcgatat ccggatccat tatctgaatc tggatgtcgc
    12301 ggcgttgccg gacgcggcag gattctacgg ggcggcgacc tcgccctacg gccggatgac
    12361 cgggctggcc gactggttct tccgtgctgc ggcgtttccc gatgcggagg ataacatcga
    12421 ggcgtatctc ggtcggtatt actacgatgg cgcgacggag gcaacggagg tcgcggagtt
    12481 cgcgcgcgca cggcgcgggg aacttttcgc ttttctggac gagttgatcg accggtatgc
    12541 gctggattca gctgatctgg tgggcttttc cctctgcttc ttccagacgg tcgcctcgct
    12601 ggcgatggcc agccggctga agctgcgcaa cccggaggtt accgttgtgt tcggcgggcc
    12661 tgcggtcaaa ggcgttccgg gaaagacgct ggtcgagcat gcgccctgtg tggatgccgt
    12721 gttttccgga ccggggctgg tcagttttcc tgagttggtc ggccgtcgtc tcgcgggtct
    12781 tccgatcacg cgtccggcga ttccgggcgt tttcgcgcgg ggtgtggcgg atgcgctgcc
    12841 gccggcgtgc aatgtcggtg cgcaactcga catccggaaa gatgtgccgc tcgactaccg
    12901 ctcgtttctg gatcattttg atgccgtgtt gggggattcc ggacgccagc cgtttctgct
    12961 gatgcagacc tcccgagggt gttggtgggc cgaccggcgg cgctgcacgt tctgcggctt
    13021 gaacgatctt caggaacggt tcgaggccct gccaccggaa gcggccctga ggcacatccg
    13081 gtcggttctc ggctatgcgg ggcgggtctc gcggttcgtg gcctgcgaca acgtggtgcc
    13141 gccgaattat ttccgcgagg tgttcccgca tctggcaccg ccgcagggcg tcttcatcaa
    13201 atacgagacg aggtccgcga tcacggcgga ggagatccgt gtgttgtgtc gggcgggcat
    13261 ccgctgtgtc cagccgggcg tggaggcgct ttccacagag agtctgacgc tcatgcgcaa
    13321 gggggtcacg gcgttcgaca atctccggtt cctgaaagac tgcctccgtc acgcgctgta
    13381 cgtggagtgg aatctcctgc tcttctcgcc gggcgagcgg gaggaggttt accgcaagta
    13441 cgagcgcgat atcccgcgcc tggttcatct gcagccgccg gagggagtct ttccgatcga
    13501 gttcgtgcgc gattcccgct atttcgaaga ggccggggcc tacggcctgg atttggagcc
    13561 gcacgaaagc ctgcgctaca tctatccctt cgctccggaa acgctgtttg gattgtgtta
    13621 ccgttttacg gaccggaacg ccgatccagt atgggccgaa caatggttgg accggctggg
    13681 cgggttgatc gagacctggc aggagcggtg ggcgtgcggt ccggaggaac ggccgtttct
    13741 gctgctctat cgcgacgcgc aaagtgcctg cattcacgat tcgcgctcgg gcggaaccgg
    13801 gcgttaccgg atttccgatc tttccgagcg gctgctgcaa gagatggggg agacgccccg
    13861 gacgatcagc gaggccgctg tcgcggcaga ggtcgaagag aatgccgtgc ggagagagat
    13921 tggatttctg aacgagaggc agttgctctt tgaggaggat ggccggttcc tgagtctcgt
    13981 catctctccc acgtaggcaa acccctctcc ttcgtgtctt gatgtgagaa acaacctgtt
    14041 ttttattggc ctcatctgcc atgtgcgagg tctggcaagt tgaaggtggc ggatgcgcgg
    14101 ggtgaccgtg tatactgttt ttcatccaac aaggagcagg gagtgaacgg accgttattt
    14161 ttcagcacat tcattttgat ctttctcgcc gaactggggg acaagaccca gttggcggtg
    14221 atgagccaat ccgcggcctc gtcctcaaaa tggacggtgt tcgcggccgg ggtggcggcg
    14281 ctggtcgcct ccaccgccat cggggtcttg gccggaggcc tgatccggcg ttttgtgccg
    14341 gacgagcgtt atattaaatg cgcgggcggc ctgctgtttc tgttgttcgg tgttctgatg
    14401 ctgcgcgagg tgttcgggac gaaaaccgtg ccggtgcgga cggcggcgcc gccggtttcc
    14461 gccttctccg actggatcgg ccgtttcgtg atccagcagg ccgcagtgtt tgagaaggcg
    14521 gcgtttgagg attatgaggc gttggcggcc cgttcgcttg acccggagga aaaagcgctc
    14581 tatcaacgct tggcgcagga ggagcgttgg caccatcagg ccatgctcgg cgcgatggag
    14641 gcgggggcag gccgggacat tccgatcacg gcggagatgg ctggcgcact gccgccgttc
    14701 aacgagatga tgcacgatgc cgtgaaggct ccccgcgaac tggagcatgc gatcgagcac
    14761 gaacgcgcca tgtcccgctt ttatcagatc ctcgcggaga agagcacgtt gccgcgcctg
    14821 cgcgaaacgt tccgggcact ggccgcagcc gaggcaaatc acgcgacccg ccttctggag
    14881 cggatgcgga atgcggagac gaagacgacg gagtgacggt agggggacgg tatggatgag
    14941 tgggtcgata tccgcaagga cgaacggcat gtggcgcgtg aacgcgcgaa ggcccgcgtg
    15001 ctgcgcaaca gtgcatggtg gcgtgagcag atccggccgg gagtgtgccg gtattgcggc
    15061 aagacggttg gcgcggacgc cctgaccttg gatcatgtgg tgcccgtggc gcgcgggggt
    15121 acgagcaccc gggggaatgt ggtgcctgcc tgcgtcgcct gcaaccgcga aaaaaatgtg
    15181 ctgaccccgg ccgagcggat tttgacggaa ctcgagcgcg acgcggcggc taagacaggg
    15241 catgacgatg tgtaacagac gcaaattgac ggtggtgacg ccgcacggtt tctgttcggg
    15301 ggtggcccgg gcggtggcca cggccgaggc ggtgctggca gcctgtcccg gcgaacgggt
    15361 gtattgtctg aacgagatcg tgcacaaccg gcaggtggtg tcgcgtctga ccgatgcggg
    15421 catggtgttc gtttccgatc cagccgaagt gccgcaggga gcgcggcttc tcttttcggc
    15481 gcacggggtt tcgcccgagg tgcggcggca ggcggcggcc cggggtgcgc gggtgatcga
    15541 tgccacctgt ccgttcgtca tgaaggtgca tcacgaggtg gtgcggttcg cggcgcaggg
    15601 tgcctgcgtg gtctgcattg gtcaccgaca tcacgaggag gtggtggggg tggctgggga
    15661 ggcaccggac caagtccgta tcgtggagtg cgaggcggag gcggagtcgc tggatgtgcc
    15721 gtcaacccaa ccggtcgcgg tggtgactca gaccacgctg gatgcggctc gtgtggaggg
    15781 ggtgatgcgg gtgttgcggc gacgcttcgg cgcgcgtctg atcgtgcccg ccagcacgga
    15841 tgtctgctat gccacgcgca accggcagcg ggcggtgagc ctgctggcgg aacgttgcgg
    15901 tctggtgctg gtgctgggat cggagaacag ttcgaacagc cagaggttgg tcgagaccgc
    15961 ccggaccgcc ggggcgcggg catgccttgt cagcacgccg tcgtgtctgg cggaggtggc
    16021 gggcttggca gacgagcctg cggtcggtct gactagcggt gcctcgactc ccgaggcgtt
    16081 tctcgatgag gtgatccgcc tgttgtgcga gggttacggt ttccccgcgc ccgagatcct
    16141 gtgcgcggtt gaggaggaat cgcatgtgtt ccggttgccg ccgctgccgg aatgattcgg
    16201 ggaggagacc catgcgcgga acggtttggg aaacgcgcca ataaaatcct ttcttgtctg
    16261 gggagtcttt gttttaatac ggccgttttc ggcgcggaaa gaaaaaggag aggtcatgac
    16321 ggatcctgag tgtgtgtttt gccggatcat tgcggggcag attcctgcga aagtgttgtt
    16381 cgagaatgag gaagcgatcg cgtttctcga tatcgcgccg ttcgagccgg ggcatgcgct
    16441 ggtggttccc aaaatgcatg cggccctgtt cacggaattg ccggactccg tcctgtcgca
    16501 cctgatgccg ctcgtcaagc gcgtggcgtc gttgctgttg gagcgtctgc cgtgcgacgg
    16561 gttcaacatc ctgcagaata acggacgttg cgcgacgcag gtggtgcccc atgtgcattt
    16621 ccatgtggtg ccccgttgga acggacgcgc gatcaattgg atttcgggcg cttacgcgtc
    16681 tgcggaagag atggagtccg tgtaccggaa actgactgtg gcggctggcg tatgaagggt
    16741 cgggatatct ctttccgaga ggttgtgcag gggatctgcg ccaaggatcc gcgttatgac
    16801 gaggacgcgt acctctttct gatggaggct ctggacagta cggtcaaggc catccgggaa
    16861 aaagagcccg aacacggccg ggatgtgagc ggacgggaac tgttggaggg aatccgggcg
    16921 ttcgcgcttg acgagtacgg ccctctggcg tacaccgtgt tcgccgagtg ggggatacac
    16981 gaaactgcgg atttcggcgc gatcgtgttc aatctggtcg aggcgggccg gttggggaag
    17041 agcgagagtg acagcttaga ggattttaat caggtttatg atttcgacga ggcgttccga
    17101 ctgccgttcg agccgcagga gccgccggaa aaggacggtg cggcaggcaa acgccaacgc
    17161 ggctgaggcc atttcgcgct gcggcgcgtt ggatcacgcc gtgatgttgc ggtcgtatcc
    17221 gaagacctct cggaatgccc ggaccacact ctgtttgacg gcgggcatgt cgggcgcttc
    17281 ccccagcagg tgttgcagcg aggtgacgct ggcatccgtc aggccgcaag ccacgatccc
    17341 gcggtaatca tcgagccggg gcgccacatt cagcgcgaac ccgtgcatgg tgacctgatg
    17401 cgcgacgcgg atgccaagcg ccgcgagttt cgcgttaccg acccaaacgc cccggttccg
    17461 cggatcccgt gccgcgacga tgccgaaatc ggcgacagcg cgaatgagcg tttcttccag
    17521 agcggtcaca tactccagca cacggagtcc ggcatccccc agatgaagaa tcggatagcc
    17581 caccagttgg cccggtccgt gatatgtcac atcgccgccg cgtgaggtct gcacgcgttc
    17641 gatacccgcc tctttcaggc gttcctcgga atagagcaca tgcgctgccg cggcactacg
    17701 ccccagtgtg taaaccggcc gatgttccag cagcagcagg gtatccggaa tctcccgcgc
    17761 ggcgcggcgc gtgtgcagcc gctcctgcag ggcgagcacc tcgttgtatc cggcatcgcg
    17821 tccgagatcg ctgacctcca gctcacgcat gtcatccgcc tccggattga aacacaaaag
    17881 ccggtcaccc cgcccgtgcg cggtgaccgg tcttgaccca aagagaacct caggccttca
    17941 ggccatcttc tgcatgctca gcgccacgcg ggattttttg cggctggcgg tgtttttcgg
    18001 gatcacgccc tttttggctg ccttgtcgag aactgacgtg taatccgaat acagcttctg
    18061 agccgcctcc ttctcgcccg cactgatcgc cgccaacact tttttacgcg cggtcagcac
    18121 gcggcttttt acggcccgat tccgcacgtt acgcgcttcg gactgtttcg cacgtttaat
    18181 cgcacttttg atattcgcca tgatgaaacg ttactcctta atcctctttc tggaaccgtg
    18241 tgatgtcgcg tatcataacg aatccgccct gcgggtcaag cgggaagagt gatcggtcgg
    18301 cacatcgata tcgaaacgct atcgaaatca cgatccggat ctggatcgaa accagaaacc
    18361 ggaaagagcc tgtccgccag cgcgtgccgc atgccttcgt tggcctgaag gtcgtgctcg
    18421 ttcaacgttg caggttcatt gccccagaat atccggacct gtctttcagg caatcgagca
    18481 aggcgctgtc gaacttcttg ttctttggca cctgtgcccg ctgcgcctcc ggcacgactt
    18541 ccgccttcgc cagccgatcg cagggcagcg aatagcggat gcgcttctga atgccgtcct
    18601 cctcgaaata aacgtgcccg ccatgataaa acgccttctc gggattcgcc tgtgcgacat
    18661 tttcccaggc gatccgcccc gtccgctcga caatccggat cttgccgggt ccggtcagca
    18721 ggagtgcgcc gttcggcttc ttcccttcct caaatgccag acggccgaaa tcggtcatcg
    18781 tctgttccgg acgcaaggcc ggcggttccg gaatcggcgg aacgcgctgt tctccgcccc
    18841 cgtgcttctc gttgccatgc tcgatgatcc agcggcgcgc aagttctacg gcctcgatgt
    18901 ttttgttgac gagaggcgtg caatggaaga tgaacccctc cacttcgccg gcgcggaaga
    18961 gcttgagggc aaagtccaac tgaagcttca tcgacgtcgc gtcgagcggc tgggagccgc
    19021 cgaaatccca catgtagatg cccagcagcg tcggtttctt cggcgcgatc ttgcgatagg
    19081 ctttgaagtt gttctcgaga gcctggacat tcttgccgaa ccacgtccag aacatcaccg
    19141 tgtcgaagat gtccacgacc gccttcgtct cttcgggatg gtcaaggtcc cgttcgtaga
    19201 agacaagtcg catgtcgatc ggacggtcat aggaatggag ccgcttgcgc aacgcacgca
    19261 actcttccag cgtacgcgat cccggcaaca cgttcacttc cgtgccgtcc gccagtttct
    19321 cctttcgaac cggcttgcgc ggctggtcga agaagtcgtc gaactcgaac cccatgagat
    19381 tgggcatcgt tttcattttc ttttcgccga acgccagaaa ctcgggcagg atgtccctgc
    19441 cgtgaaggtg aaggtcgccg ttgtccacga cccaggcgac gcgcttcatg tccgtaaact
    19501 cggacatgaa cttctcgtct ggaatgtgcc agatgacaat ggagacgttc ggtatgccca
    19561 tgtaccggca ggcctccgcc atcgtcacgt ccggggaaac cggaatgcga aaccaaccgt
    19621 tcggctgatc atactgcccg gtcttatgcc cccacatcca gcagcggtcc ttcagttccg
    19681 gcgcatactc cgggcaaccg ctttcaaact ccgcgaagca acacgccgcc actcccattg
    19741 caacggtcat cgatatcagc gcatctctct tgcgattcat tgtttctcct gttccgctac
    19801 cctttcatcg ggggaacccg aaacaaaccg gttcccataa ttgacaccta ttatgcaggg
    19861 gggaggggga aagtcaattg gacttgtccc atgaaaagat cgctttccgc tctgcggcag
    19921 tttcagtatg ctatggaagt tgattgaaac aaaaatcgag tgagaaagga tttcccaatg
    19981 cgacacgcgt atgcggtgat tctggccgga gggagcggcg agcgtttttg gccgctgagc
    20041 accaaggccc ggcccaaaca gttcatttcc cttttcggcg gacgcccgtt gctggcgttg
    20101 gcggtggaac ggttgagcgg gctgatcccg gcggaccgga tactcatcat tacggccgaa
    20161 catctcgtcg aacaaactgt ggaggccgcc tggcatgtgc cgcacgccaa catcatcggc
    20221 gagccgtgta aacgcgatac cgcgccagcc gtcgcggtgg cctgcggcct ggtgcggcag
    20281 cgcgatcccg agggtgtggt ctgtatcctg accgcggacc agttgatgag cgatgtggag
    20341 accttccgcg tgacgctggc ggacgcgatc cgcgtggcgg gttgtgagga ggcgatcgtc
    20401 acgatcggca tcaagcccga cacgcccgcc acagggttcg gctacattga ggtgggcgag
    20461 gcggtcgaaa cccggaccgt gaccctcttc aaccgggcca agcggttcgt ggagaagccc
    20521 gatgcgtacc atgccgcgcg gtacgttgaa agcggaacct atttctggaa tgcgggcatg
    20581 ttcatctgga aatgcgacgt catgcgcgag gcgctcgagc ggttcgcccc cgatctggcg
    20641 gaactctgcg atgcggtggt tgcggccgcc ccgggtccgg aactcgcgaa tctgctgcaa
    20701 agggtctacc cgtcgctgcg cgccatctcg atcgattacg cggtgatgga acacgcaccc
    20761 aacatcctgg tggcccgagg agagttcggc tgggatgacg tgggttcctg gcccgcggtc
    20821 gcgggacact tctccgctga tctggccggg aacgtggtgg tcggctcgtg cgagccgatg
    20881 gaatcgctga acaacatcgt ggtctcggaa aaccggctca ccgcgctgat cggggtcagc
    20941 aatctggtcg tggtgcagtc ggacaacgcg acgctgatct gcccccagga tcgggcagag
    21001 gacgtgaaga aactgttgcg ccggatcgct cagcggccgg acggcgaaaa gtatgtgtag
    21061 cccccaacgg cagagggtgt gacagcgatg gcggcgaacg gacagcgcaa gcccggtggc
    21121 gggccaacgg cggcaacgct ccccggaccg gtcgcgcgtc ggttcaggct gactgtcgcg
    21181 tatgacggta ccgcgtacgc gggttggcaa gtgcaaccgc accaccctag cgtgcagcag
    21241 accttggagg aggcgctcgc ggggattgtc gggcacacgg tgaaggtgca tggaagcggc
    21301 cgcacggatc agggggtgca cgcgcggggt caggtggcgc atgtcgatct cgtgtgccgc
    21361 atgacgcccg tggcggtggg ccgtgcgctg aacgcgcgcc tgccgcagga tatccggata
    21421 cgcagcgttg cgcgggtgcg gccggatttt cacgcgcggc gttcggcggt gggcaaggag
    21481 taccgctact tcgtgtggaa cggcgcgcag ctgctgccgg accggcggct ttatgcggcg
    21541 cacgtctttc gcccgttgga tgtggaggcg atgcggcagg ctgccgcgtt tttcgcgggg
    21601 cagcacgatt tcgcctcgtt caccgccaat ccgcagcgtg aggtgccgag cacggtgcgc
    21661 cgcgtgttcg atgtgacggt tggcaaacgg ggacgggaga tcgtcttccg cgtgcgcgga
    21721 gaaggttttc tctacaagca ggttcggagc atggtgggat tcctgctccg ggtgggtgag
    21781 ggggcggagc cgcctgaggc ggtacgcgac ctgttggcgt cccgtgcacc gcgtaaggca
    21841 cgggtgccgt ccgcaccggc gcgcgggttg tttctctggc gtgtgtggta cgcgtgaggg
    21901 acccgggcag gccagcggtg cggattgaca cgggaaagga cggggcagga atgggcgagg
    21961 ggcgggaacg gatgcgggtg tcggcaaacc ggaagcccgg agaactgatc gccgcattgc
    22021 aggcggtgca ggacgcctgc gggtatgtgc cgccggaaga actcgcccgc atagcggtaa
    22081 cgatgggggt gagcgaggcg gagttgaccg gagtggtcac cttttacgac cagttccgcc
    22141 tgcggcccgt gggacggcat cggatccggg tgtgcgtcgg cacggcctgt catgtgaaag
    22201 gcgcggagcg cacagtggag gccttccggc gcgcgttgcg tattcccgag gggtcggaca
    22261 ccgatgcgga aggagagttt acagtcgaac gggtggcctg tctggggtgc tgcatgctgg
    22321 cggtggccgt acaggtggat ggtgtcattt atggacaggt gacgcccgcg cgcgtgaaag
    22381 ccgtgctgcg tgacgtgcgg gccggggaga cgcagatccg cgacacggcc gacgtgccgc
    22441 atccccagac aggtgcgcgg gtcaggttgt gcgcctgttc cagttgcgcc gcggccggcg
    22501 cggcggatgt gctgcacgag attcgccgcg tggtgcggac ttggcgtttg ccggtcgggg
    22561 tgcgcgaggt cggctgcacg ggcggatcgt accacgcgcc gctgatggat atcctgttgg
    22621 aggacggcct ccattttcgt tacggacgtg tccgcgtccg ggatgtcgag cgtattttgc
    22681 tgagccacat ccggcccgcc cggactgcgg cgcggctgca ggccacactc cgggcatgga
    22741 ccgaacagct ttttgcgggc gaggaggatg atccgcccgt ccggtttccg ctgagcgtcc
    22801 gggatcagag tgatgccggg tactggctgt gtcagcaccg catcgccacg gaaggcgcgg
    22861 gcgaaaggtc gtcgctggac tgggacgagt attgccgcca cggtgggttc gaggcttacc
    22921 gtacctgcgc gcggcagatg acgccgcagg aggtggtggc gcaactggaa gcctccggct
    22981 tgcgcgggcg cggtggcggc gggtttggga cggggcggaa atggcgagcg gtggcggacg
    23041 ccgcgcaagg ttccgatgcg gtagtggttt gtaacgggga tgagggggat ccgggcgcgt
    23101 tcatggaccg catgctgttg gagtcctatc cgttccgcgt gatcgagggg atgatgatcg
    23161 cggctcacgc ggtgggggct tccgagggga ttttctacat ccggcacgag tatccgctgg
    23221 ccgtacggcg tgtgcgcgaa gcgctggcgc tactggaacg cgaagggatt ctgggggcgg
    23281 aagtgccgct ccggttacgg gtcgtcgaag gcgcgggcgc gttcgtgtgc ggggaggaga
    23341 ccgctctgtt gtccagtgtc gaggggcgcc gcggcgtgcc gcatgtgcgg ccgccgtacc
    23401 cgtccgagag cgggttgttc ggacggccca cgctggtcaa caatgtcgaa accctagcgc
    23461 tggttccgtg gatcgtgcgt catggcgctg cggcgttccg cgccgtgggc acggcgggga
    23521 gccccgggac caaggctttc gcgttggcgg gcaaggtcgc gcgtggcggg ctggtcgagg
    23581 tgccgatggg tctgccgctg cggcaggtgg tggagcagat cggaggcggg gtcggcgagg
    23641 gccaccggtt caaggcggtt caggtcggcg gaccctcggg agggtgcatc ccggcggact
    23701 tgtcggatat gccggtggat tacgagaccc tgacgcgggc cgggggcatg atggggtcgg
    23761 gtggaatggt ggtgctcgac gagaccgact gtatggtgga tatcgcgcgg tattttctct
    23821 ccttcaccca gcgggagtcc tgcggcacct gtaccggatg ccgcatcggc acgcggcgca
    23881 tgctggagat tctggaacgg ctctgcaacg gagaggggcg gccggaggac ccggcggcct
    23941 tggagcgtct ggctgaaagc gtgcgggcgg ggagtctgtg cggattgggc cgcaccgcgc
    24001 cgaatccggt gttgtcgacg ttgcggcatt ttcgtgcgga agtggaggcg catgtgcgcg
    24061 gcgtctgtcc ggcgcgcgcc tgccgcgcgc tgatcgccta tacggtgtcc gaccggtgca
    24121 tcggctgcac gctctgcgcg cagaactgtc cggccgaggc gatcgccttc accccgctgg
    24181 agcgtgcggt gattttgcag gaccgctgta cgaagtgtgg ttcgtgccgt caggtctgtc
    24241 cggaaaaagc cattgaaatc gaggatgcga aacgggataa tgaaatccgc tgaagaggag
    24301 ttgagcatgc gccgcacaaa aaagacggtt ccgggagtaa cccggttggg aagccaaggc
    24361 acggtttacg ccgaccaata tgacccctcg gtattagagg ctttcgccaa ccagcaccgg
    24421 gaccgggatt actgggtgac cttcacgtgt cccgagttca cctcgctctg tccggtgacg
    24481 ggccagccgg atttcggcac gatcatcatc cggtatgtgc cggatcagcg gatggtggag
    24541 agcaagtctc tcaaactcta tcttttcagt ttccggaacc acggggattt tcatgaggac
    24601 tgcgtcaatg tcatcctgaa tgacctcaag gccctgatgg accccaagta catcgaggtg
    24661 tggggacggt tcgccccgcg cggcgggatc gcgattgaac cgtacgtcaa cttcggacgg
    24721 cccgattccg cgttcgccgc gctggcggaa acccgtttcg cgactcatcc cgcagcgcgg
    24781 tgagtttcgg acagcgcgtg tgcaactccg gaaaagcgga ttgttcaaga aagaggattc
    24841 catgacacgt caaattggat tcacggatac ggtcgttcgc ggtgtgcgcg tgttgctgtt
    24901 ggcagctctg tggtgcgccg gagtgggtgc gcgggccgca gaaccgacga ccgcgccgtt
    24961 cgcggggaaa atccgcctcc tgcttccgcc ccgcctgtac gcggttcccg gcatcgaaat
    25021 gaacgtttac ttcgacaacg tctgcctgat gcccaatcca gagagcgtcg tgttcgatgt
    25081 gacctgtacg aagggacggc agcaggttga gcgctggaca ttcgttcccg aggaggagga
    25141 tgcgggtgag tatccgttcg tcctcgaagt ccgggatgcg gcgaacacca ttatcgcgcg
    25201 cgcggaatcg cggttgcggg tcacgccccg cgcggccggc gcgggagcgc cggtcacggt
    25261 gttgacggtt ggcgacagcc tgacgcatgc caccgtctat cctgcgcatc tgctggagtt
    25321 gtgtaaggcc gaaggcaacc cgcacgtcac gctggtcggg catacgccct ataccaaccg
    25381 ccccgcgatc cgcatcgaag gctatggcgg gtggaccgcg aaacagttca cgaccggtta
    25441 caaagagggc aagagagata cgggttcgca agactggcgc gtgtggaatg cgagcagcag
    25501 tccgtttctg tacgcggatg cgcagggtgt tcccgcattg gattttgccc gttactgccg
    25561 ggaattcaac gggggccgtg cgccggattt cgtcactttc ctgctggggt gtaatgacct
    25621 cttcggcgcg gatgacggca gtatcgacgc gatcctcgac gccatgttca agcactacga
    25681 tgcgttgatc gagatggtgc gcggggtcag ccgggagacc cgtatcgggg tgctgctggt
    25741 gacgccgccc gcaggtacgc aggatgcgtt cggtgccaac taccgttgcg gcctgacccg
    25801 ttggcagttc aagcgcaacc agcaccggac ggtggagcgg atgatggaac ggtacggcgg
    25861 gcgcgagggg gagaacctct ttctggtccc cgtccatgtc aatctcgact gcgtgcacaa
    25921 ctatcccacg ctgcaaggcc cgtggaatgc cagaacccgg gaggcgggca gccgactgaa
    25981 caatggggtc catccgtcgc gggagggata tctgcagatc ggggattccc tgttctgctg
    26041 gatcaaggcg caactcgtcg ctgacaattt ttgacatgat taacgtgatt gacaagattg
    26101 ttgtgccgtg cacggcgtgg cgccaccgct ttccctccgc gctgtgcgcg gatacacact
    26161 ttttttcagg cttgcagtca cgacgccgtc ccgtaaaaac ctctgcgtct ccgcgtctct
    26221 gcgttaaaaa actttcaacg ctcaactctc tgcgggcggg gacgcccgcg ctcccgactg
    26281 cgtacgcaca aaacacgcac tcgactcgca tgacctctgt ctctaaccga ctacgaaggg
    26341 cgatgtgttc cgggcgtggt ttctcggatg acgatttcca tgagggttat gatgaggaaa
    26401 aataaggcag tggtgatttt ttcgggcggg caggacagca cgacctgtct ggcacaggcg
    26461 atccgcgact acggggaggg caacgtttcc tgcatcacct tccagtacgg gcagaaacac
    26521 agccgcgagg tggaggtcgc caaggaaatc gcgcggatct ttcgcatctc cgaccacccg
    26581 atcctcgctc tggactggta ccggcaagtc accaccaacg cgttgctgga ctccgatatg
    26641 gcgattgcgg cagggcaggg aggcgcgtgc ccaaacacgg tggtcgacgg ccgcaacatg
    26701 ctcttcctgc tggtcgcggc gatttacgcc aagagccgca acatccggga ccttatcatc
    26761 ggcgtgggcg aaacggacta cagcggatat cctgactgcc gggatgtgtt cgtgaaatcg
    26821 tgcaatgaga cgctgaatct ggcgatggcg tatccgttcc gggtgatcac gccgctgatg
    26881 catatgacga aagccgagac gtgggcactg gcagacaagc tggggattct ggagttggtg
    26941 gcggaaaaga cgctgacctg ttacaacggg gtggtgggac gcggatgcgg cacatgcccg
    27001 gggtgtctct tgcggcagcg cgggtatgag gcctacctgg cgcgtcgggc gcatgggtga
    27061 ggattaacag gatttatgat ggaagtgatc ggcaaggagt tagggggtaa tcatgctgac
    27121 cgtgtcgaag cagtgccagt ttgatgcggc gcacgtgctg cctcatcatg cgggacagtg
    27181 caaaaatctg cacggccaca cttaccgcgt gatcgtggaa gtcgccgaag acgcggacag
    27241 cgaagacatg gtgatcgatt tcaaggatct caagcaggtg attcgtgatg tgattgtgga
    27301 tcgctttgat catgccttcc tttacgacga gaccagtgaa agcgagtgcg agattgcggc
    27361 ggtgatcgcc aagcacggca tgcggagcgt gggcttgccg ttccggtcga ccgctgagaa
    27421 tctcgcccgt tatttcttcc atgaactggc aacgcgcgtc aacgtggtct ccgtcaaggt
    27481 ctacgagacc ccggagtcgt gtgccgaata ccgcagggga tgaccgcgta tgtgtgagcg
    27541 ccagagctat ccggtggtgg agattttcga gtccctgcaa ggcgaggggt acaacaccgg
    27601 cctggagagc gtgtttttgc gtttcggcgg ctgtaacctg aagtgcccgt ggtgcgacac
    27661 ccggcacgaa actttcgaaa cgctggacga ggagacggtg gtggcgcggg tgaccgcatg
    27721 ttccgcgcgc gcggtgattc tgaccggtgg cgagccgttt ctgcaaacgg ggctggaggg
    27781 gctcctgacg cgcttccggg atgccgggtt ctggatcggt gccgagacca acggggtgtt
    27841 ggcgccgtcg gcggaggtgc tgcgccgcgt ggattatctg acggtgtcgc cgaaagccct
    27901 gtacgcgccg ctttacgatg acgcgcgtat ggtgcgccgt gcggacgagg tccgcatcgt
    27961 ggtggacggc gaggtacggg atttctgcat ggcgatgcgc aaccggatcg aggctccgcg
    28021 ttactacctt tcgccgtgcg agcgcgacgg cacgttcaac atcggggaga cggtgaggct
    28081 gttgggcgaa ctcaaccgcg gccggcgtag cgggaaatgg cgtctgtcgc tccaaacgca
    28141 caagctggcg gggttccgct aaacaaaagg ccggtcgtca gaccagcctt ccggtgttta
    28201 tggtaggggt gcacggactc gaaccgtgga cccagtgatt aagagtcact tgctctacca
    28261 actgagctac acccccataa aaagcatgga gcgagtgatg ggtctcgaac ccacaacctc
    28321 gaccttggca aggtcgcgct ctaccaattg agctacaccc gctgcaaaag aacggacgta
    28381 ctttatcaga agcgtgcggg gcatgcaacc gcaaaaccag tgatttttgc agtttttttg
    28441 acgggacggc agcgcggttt ggcgcacgga gatcagagaa acggaaattc gcgtccgcag
    28501 gcagcccgga cgcggcttat ttcggacgcg cgaggcgttt gcggagaccg tccacggtga
    28561 acgcggcgag atcttcgaga atccagtccg cttcggaaag ctcttgcggg tgcgatccgg
    28621 ggcgagcgag ggccacgcag agcatgccgg cccgtttggc tgccagcacg ccgacgcgcg
    28681 aatcctcgaa aaccagacag tgttccggcg ggacgccaag acgccgcgcg ccgagcagga
    28741 agccggcggg cgacggtttt ccgggctcgt aatcttcggc acccagaaca aaccgcacat
    28801 cgcgctcgat ctccatcagg cgcacggcct cttccaagtc gtggcgcggg gaacccgaga
    28861 cgaggatcac cggatagtcg cgagccagtt gtttcaggag cgtgacggag gaacggatga
    28921 cgactccctc gccctgaccg cgcaagcgtt cgtaatacac gcgcagatgc tccgccagcc
    28981 gatgccgcgg aatctccgac agcaccggga aacgggtgac caacgcctgg taaatgtcaa
    29041 tccacgagtg accgtagaca atacggagga cggtttcggt gtcgcaaggg cattgccggt
    29101 ccacgagcag atcccgcatg gcctcggccc agagacgttc ggaatcgacc agtgtgccgt
    29161 cgagatcaaa gaggacagct tgaagggtca tgcgacggtt tggctgcaac actttttata
    29221 cttcttgccg ctcccgcagg ggcagggatc gttgcgcccg atcttgggat ggtcacggcg
    29281 cacggggtcg ggaccgaaaa tcttgccgtc gagaaaacgc cattcgcccc cgacccggcc
    29341 gaattccgcc cgctcgtgat ggttgaaatc cgtctccttc acggagtaat gggcgatgaa
    29401 ctccaccacg ccggtgtcgt cgttctcccc gccaccttcc agcgacacga tctccatgcc
    29461 ggtccacttg gcgtttttgg cccagttgcg actgctttca gcatcaaatt ctttgcgcac
    29521 cttggcccct gaggtctggt agagatagtc gatatccccc atggcgtagg cacagtaacg
    29581 ggaacgcatg agagcctggg cggtcagcgg tttttgacgc tcgagcagga acggttcaca
    29641 gcaggactcg aactcgatgc cactgccgca gggacacaaa tcgctcatcg gaaagcctcc
    29701 tgaagaccgg ggacaactcg gttggccatg tccggtcaca acggaggtgt actgtatcgt
    29761 atgcgcctct tccccgcaat cccgaaaaca ccgaatcgat acgctgttgt cgtgggaatt
    29821 tcaaaagcgg acggttggat agagcaaaag cggaaaccgc g
//
LOCUS       DAOSRB010000010        28792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1794_length_28792_cov_7.244145, whole genome shotgun sequence.
ACCESSION   DAOSRB010000010 DAOSRB010000000
VERSION     DAOSRB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 28792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28792
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1794_length_28792_cov_7.244145"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1661)
                     /locus_tag="PLZ74_01200"
     CDS             complement(129..1661)
                     /locus_tag="PLZ74_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOR96971.1"
                     /translation="MNRWCVGGMLLVCWVAGWVQAEPLEQGEARFLKARLVPAPQSVT
                     LTDGPRVLLNGALRVSIACVTNGEAAVTETRRVFKEWFGHTPKIGLRHADADGLPQKP
                     DAYRIEASGRTLLIEAADSGGVRNALRTLRQLAEPLRGTREVAEYAIPATRIEDAPVM
                     AFRGFHLCWFPENTPVQMERFIRLAAYYKMNYVVIEPWGMFVSGRKPGLAWPEGTMTR
                     KALRHLVKVADSLGVTLIPQFNALGHASGARMSCHKHAVLDLNPELQPLFEPLGWSWC
                     LSNPETIRVIDECVTDLHEAFGRPPFFHAGCDEADDMGTCPACRRTDYPARVRDHLTH
                     LRDLLAARGCRMMIWHDMLLRSGDPRWKGFYANGKEGAERLLEGLPRDIIICDWYYGA
                     PNKEGTWPTLRYFKEKGFGVLACPWENAEGYRGLGKAVVSEGLEGMLSTTWHHLYGRD
                     MMGIYFRAAHAMWGTRPNGGHEYLVFAHHLRQIGWDVPVRTYEDTGTYHTQLPEKTAS
                     PH"
     gene            complement(1720..2829)
                     /locus_tag="PLZ74_01205"
     CDS             complement(1720..2829)
                     /locus_tag="PLZ74_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96972.1"
                     /translation="MTLTSQQIKEFAASLGRPCLVGVADIGRFRSAPRQMHPANIFPE
                     CKSVISVVAPFPRGTLRGITEGTYWPNYTYYSYNRLNNLFRPRITYALSCFIEDHGWE
                     AVPLYPAVPERGGMHRPVKPGRPAREINLNVRIAAVAAGLGEIGWSKVFLTRKWGPRV
                     RIGTILTDAELEPDPLIKPGTLCNRCMKCVRTCPGRAIPSPGERPPVRVEIGDQVYEW
                     GDVSMGRCTLTHHGLNRQVSPFLVQDFPTVDFKVPESRVSEELAYRMAYTLATGTWVA
                     SEEFPHGNIVDYYHQILGHVGYFAICGAQGCVRGCMDSLEKRHAIEQSAFKTAVSPRG
                     QWTLRQPEDEESAGIAEGTCAELYRQPDLQAGRWS"
     gene            complement(2837..3787)
                     /locus_tag="PLZ74_01210"
     CDS             complement(2837..3787)
                     /locus_tag="PLZ74_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR96973.1"
                     /translation="MKTEEVKAMARECGADLVGIAPVGRFAEEPPERNPAAIFPECRS
                     VIVLGRRICRGSLRGVEEGTNFGSTYQCFGYTWVEDHFLSQTSYDLSLRIEEQGFEAV
                     PIFAYNGAGMPKGVPVAPDRPAPNVYIDPRVAVVAAGVGEIGLGGFLLTPEFGTRQRF
                     AFILTDAELEGDAVRAKTLCGDCGACAEACPFGAIHLDQIQTITVQGQEMPVARVESG
                     LCATCPNGAFLAAGRGKEPDRIAAACGRECLVRLEQSGKLGNRFEQPFRKREPWQLDA
                     LKRPLTRDAAGVLRASDVGCGAAGGTGVVAVHATGQIFSK"
     gene            complement(3823..4566)
                     /locus_tag="PLZ74_01215"
     CDS             complement(3823..4566)
                     /locus_tag="PLZ74_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOR96974.1"
                     /translation="MEKGEAQIIKSRMIPYPKQVTITAGPVVKVDRELAVTVTLKDED
                     CGLKKRVSRLFKSYFGVSPKLTFKTDASLVIPQDGYRFKIASGSGLAIEATGEGGVLN
                     ALKTLRQLAEPERGVEKMRCYFLPEMAVEDAAQWPTMQYFHEKGFPVLACPWKNTEGV
                     RSQARLVNEKGMTGMLCTTWNYMHSLDMYRMLYAGAHATWGYDGTGWNASSAFNRHVR
                     QIGWDIPVKRYRDTGVAQWQVPPENALLH"
     gene            complement(4786..7284)
                     /locus_tag="PLZ74_01220"
     CDS             complement(4786..7284)
                     /locus_tag="PLZ74_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR96975.1"
                     /translation="MKPSANSFEPFPFQSVEALRASARRLKLDLPLSGETAVLAEPLR
                     FGGRIAPNRFCVQPMEGCDAEPDGAPSVWTRRRYRRWAEGGFGVIWMEAAAVEGRARS
                     NPRQLWITRRNVRVFAEMVAELRQCACTCWGHEIAILLQLAHAGRYCRPEGVPAPVFI
                     LPDPDPAGIPESLAGQTGLSDEELDRLQDATVTAGLLAMEAGFDGVDLKACHGDLHAE
                     LLGALRRPGRYGGSLENRSRFLREVAVRLRSARPEMIVACRLSPAKEGGEERERIRLL
                     ATAGVGLFNIADSGWTAGDRKKRVNPLRRFMRLAERTQAIQQMVPEIPVVAEGFSRFR
                     RFLPQVAAGVLRRGGGSLIGIGRAALADPGLAGTVLRNGEPDLEACCIDCGACVQLVR
                     DGGRSGCVVTDRAVYGEEYARCRAISLERLREEARRCRGCEPAPCRAGCPTRIDVPAF
                     LRAFAKREDAAAYEIIRRSNVLPGLCASLCPVGTLCEGRCVAGTLDGVPVPIHAIQHA
                     VYRYAHANGLTGLRLPEKPSGRKAAVIGGGPAGVACAVTLLERGHQVVLFERASRLGG
                     TPELAIRDSRFTGAHEEAWAVLMPALRAARLTIRFGVEFGREVTPDSLRRKADAVFLA
                     AGVWGERSVGTAEGVVAGLAFLRQIKTRTRQTVPERVILLAGGDSAMDCARVALEAGA
                     RELLIVYDGPLSEMHWHMADAWFRTAGVHFLTLTRPLGYRTGKDGKVTGLRIRKILES
                     DSDGGPVPETLLEAGLVIEAMGLGLEESLVSALPGCAFNAEGLVKTAEGSLRVSSLPG
                     FFAGGGVINGGASVAQCVAEGMRAGIEMDAELRG"
     gene            complement(7303..8289)
                     /locus_tag="PLZ74_01225"
     CDS             complement(7303..8289)
                     /locus_tag="PLZ74_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOR96976.1"
                     /translation="MRKHTKSRCPQTRAATGQVFETARLPIRVGRLLRMWACVLAGFL
                     PAVAAEKRSPNDAAAPIRGVDALPREQRRAFLRGTSADWRNDTDAARALAPPPIQKAY
                     PADAKRVDLVAPERFTVGDVSAREVIGNRRSRREFSAASFSLEELSYLLWATQGIARI
                     DRDGAGQVTAHYRTVPSGGARHPFESYVAVSRVTGLAPGLYRYLPVEHQLLLLREDAD
                     IHDRVMAACYGQTFIGQAAVTFIWSAVPYRTEWKYGVIAQKLIAIEAGHVCQNLYLAV
                     ESLRAGVCSSMGYSQARLDALLGLDGTEEFVVYLASAGKCGEAPDSGGTPEK"
     gene            complement(8267..9247)
                     /locus_tag="PLZ74_01230"
     CDS             complement(8267..9247)
                     /locus_tag="PLZ74_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOR96977.1"
                     /translation="MRESMDAIRREVGKVIIGQEDVLNQILMCIIAGGHALLIGLPGL
                     AKTLTVNTLAKVLDMKFKRIQFTPDLMPSDITGTEVLETDPQTQQKQFRFMQGPIFCN
                     ILLADEINRTPPKTQAALLEAMQEKQVSVGKEIYKLDKPFFVLATQNPIEQEGTYPLP
                     EAQLDRFMFNIFVDYPTYEEEVTVIDRTTMAVGSEPNVVLDKQAVIHLQEVVRKIPVS
                     QHVIRYAVSLVRATRPGDEQAPECTKKYVSTGAGPRAGQFLILAAKARAVLEGRIHVS
                     CNDVRGAAMPVLRHRILTNFSADSEGINSVEIVKRLLEEVKEPGAETYQK"
     gene            complement(9350..9622)
                     /locus_tag="PLZ74_01235"
                     /pseudo
     CDS             complement(9350..9622)
                     /locus_tag="PLZ74_01235"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
     gene            complement(9656..10378)
                     /locus_tag="PLZ74_01240"
     CDS             complement(9656..10378)
                     /locus_tag="PLZ74_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96978.1"
                     /translation="MNGVFVQCVWGVLLACALSVTGAEERSRLYTATDPSATGGLKGR
                     IVRPSQPILQVLAMPPDEPRFVYQGTVTGGDRQEFLFQNLPMARYNLFVIYENEFFEG
                     LELSSEPDTLTPKDRESITRIVNASDPFFNRKVIHRMAGTTGRGNFARCVVTQYRDGP
                     GMVKGDDTALEAGKGRRTFKLIWLKDVGVGWQVVQKRDLYPVTVELKLLTPAHHYSKQ
                     LSRIRVTNRIRDLGELDLRKEK"
     gene            complement(10425..12686)
                     /locus_tag="PLZ74_01245"
     CDS             complement(10425..12686)
                     /locus_tag="PLZ74_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96979.1"
                     /translation="MKTMMRNAIRGLLPAVLAAGLCCRAAEPGEWAVRDATIRFVVGL
                     TRAPSHPSAGFFVKLPDGGSLPGPFPETLAFDEAGNPLTSAVLWHAKEIGCAVVFAAP
                     KEGKTAVLYVRGAKKLATWTPASGLTPSAIRCEVNGTSAKAAAQKLSRLGSVDAKTHF
                     HNQAWSAGTWQGESVPMAVCEWKPGGNAIYLLAHIEVTAPGPTWVAPMIRSGAMEIVI
                     DGKPVPLSKKNEKQGGLGGEVNLTAGLHRVELFGFSPRGQAVGPMMFTWRTSKTTMEE
                     LGGPRASDLRYPGTSMYETAKIKDEHVVKTGDCTLFEIQSQTGLTAAFEAVPDCLFAL
                     EGEEPRVAYTLKAWTKGNPKETRYSWSFEEAPNALADGPTTHWLFKGSRHSRITLTAE
                     AAGRRATCTTTLFAYTFDSSSLDDEETRHAFKRACYSMLKAYPEKADPTAAWDDAMLN
                     NLFRVLDLSGANLLVETIVTQRWPFFKKRLTPERQGLLEDIFLFSLGPRKPQEAIRWA
                     LKLSSEEFSSGRAAVLKLKAAEVMMFYLNDLAGARKLITPLLRDSGEAGEWAKIRMGD
                     LEFLERNINAATQRYGDVQSRAKATAASLTPKRLSDLSATGAARARRSGGKGGESGPA
                     PMAQPADLSAWKLSAIRDAAASEQVNILIEQGFYLEAFQALQAWERIFPLSKLTGDYL
                     LCEAKLYRALKDSKRARVILSAYCDQVDASNFLVAAMELNKQCMMDLNEPAEVIEKYT
                     EEIRRRTFAGGDQ"
     gene            complement(12726..16328)
                     /locus_tag="PLZ74_01250"
     CDS             complement(12726..16328)
                     /locus_tag="PLZ74_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96980.1"
                     /translation="MAPSVLIGQLEKIGRLLNRWQAEFRLTAGLALFGALVFCLAGVD
                     LLVRYGRNGRLLAWAVLVAGVGVTAGLVFRALARRHTPEAVAVRVEQTFPQLDNHLIN
                     FLLFSAFRVKDSFVAAYVKMDIPHWSGLDFRMMKDRRLLRRAQLALGASLLLLLLPYP
                     FAGRAWPVAMARVLNPFAELTPVSLTRILRVSPGDATVLQGGVLTLACEVQGRAGHTV
                     LLDVRPSDGEQKTYRLGTLRGGAEERFQHVIPKVTTDLKYRFRAGDAYPPGWSTITLR
                     PPLAFRAVTLKVTPPAYMRLPTKRVDAQATALDIPAGSRVELAARCNAPLRSLVVSGW
                     GGDPVEFVEQGKKPGGSVALVVTNGAAFILTAESAEGDKAESTVGFNLLPDRPPALTV
                     RYPQKPVPLPPGMAPTVDFSASDDFGFDEISIVQLPKTGESEARPKVLKTFSAAEIRG
                     KEFSALWKGVVRKTTDTGTWVMRVEARDNREGNGQTTVSGPLVFELDETDQAAKKREE
                     LAKQSRESLERVILLQRENLRKVTQLRDALANSTPEQWAETATRQEEIRQTVRLLLER
                     GGGKSLGNLVPTIKKLYLNELHEVIPTLRSVPATNGITEKDRRVGKALTMQEKILRQL
                     TFAGESAAKAQENQSASSLIGMLDGIITGQAKVLQTASQCATQGVAVAASLVDEQDAL
                     SSEVVAFIQACGAEAAAGQGEDKSQSAFLEGVAAFCKQQKITDDMLLSAEQLEQNAAR
                     EAIPHARSAFDKLTIVRKRFEEVQAQEEKESREEMIEALQIAGAKLEKLKAAEKRLKE
                     EMDKIAENRDRNTEDFDVMEQEAEEIRKNIEDAMLQIPRDLDIFAHLNVGNDLVEDLY
                     SVFEEVTQEKDSGTKDQEGGPVKEKAVMKREHYLEAMEKAEKLIEDVEMWLGDKPDTT
                     KVTDEAFDKEEMPEGVALTPLQTAMEDIIGDLLEIDEKMDREADDGAINQALPDMEMG
                     ALITEGDTTTFSAKGKSGNTTPDHKEQDGRSNVGRQGMSSGESAAGSGTISEGDDNIE
                     ARRTQDPTQSGQVVADGEAQTKATGGGKLGSGKGDDWGDSGGTERMDASEAGSWAQFM
                     EQMARKADQSYAQASLKGLRTDSLKTAAHHIRQASDAVSKGVPIGQVAELRRKAIGAL
                     KKAKTELGEGSATTLDGGVAVSTLTDVIEAEQDLAPAKYRELNADYYKKLNKEL"
     gene            complement(16373..17026)
                     /locus_tag="PLZ74_01255"
     CDS             complement(16373..17026)
                     /locus_tag="PLZ74_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96981.1"
                     /translation="MKRIGKGAVWLCGVVLVSALRAAPAETNYCYNSDFTSAKGPLDG
                     WNIDYDWTLIKKQTGNAKNASVLPMFRAKERVLKMAVPSGYETKVETPLIPYEPGDRY
                     QCTFDILVENNLEVHILFLGYNLKPGISPSEPPKLQNLRRVYKAEYVAATAAAWKTMR
                     VTIPNETISPVAYSHLKKVRYITVFMFVPGSTYLRDGNFYVSNLKIVKLPEKVKVTK"
     gene            complement(17052..19253)
                     /locus_tag="PLZ74_01260"
     CDS             complement(17052..19253)
                     /locus_tag="PLZ74_01260"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOR96982.1"
                     /translation="MIIYDHLAPTAVIAAGVLAALAATGVGYGLFVKRDGPMWVMLLS
                     RVCFFLLLGWCLLLPGEKRVETLEQRTRFLVLVDESRSMTMTPVKGMTNRWQTAQTVL
                     QMPWAAGMAEKCEVEVYPFSADLQQKTSLPELFKREPEGEATLLRDALKKLVGRYAGV
                     DVTGCLLLSDGLDTREAFKEWALEKRPFPIYTLPLEREAIWEEEPDIRVETLSTPRRV
                     TVGWQSELKAVLSGQGTRGQPFAVQLFKDDVLLQRIETQIPEGGGSREVVFKLDHAAI
                     GVNTYRVSLPALSGESQVDDNESSVVVQVLDAKNRLMYVEGPPRWESKYLSRVLRESK
                     QVSPSIFLKGPKGKFMTYGVSGEVAPDMGEAQLAQFKIVVLGNLSGEELGEERARALV
                     KFVEAGGSLVLLGGSKAWAPDGFAKTALKGLLPAKAFSGKAREGEFAVSLTEQGRSHA
                     AFGGDAEFWQKIPAVLSIFQGVVPSAAARVLVEVRSDSGPQPMILAQDFGQGKVVALF
                     SDSLWKWQLSADALKTNPYPRFWHQLLSWLSPKADQIEGRSWEVFLDRETCFLNEEIE
                     VTARWVGEGVPPEGAVVKAEITFPDKRVVPFAVTAREDQMLEGKAVPTFSVKVKGETA
                     GMYAVAAVSESGGQRLESEPAFFAVKPYSPESIPRPVEVSVLRAIAANSGGAYFDSAE
                     ELDRALLSVTPKRIEQSVFEYKTYWQHWAILGILITLIALEWILRKFQNLT"
     gene            complement(19250..21454)
                     /locus_tag="PLZ74_01265"
     CDS             complement(19250..21454)
                     /locus_tag="PLZ74_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOR96983.1"
                     /translation="MISFLNPLFLAALGALTLPLILHLIQSSRTERLPFSTVRFLKLA
                     QKRSSRRIRMENLLLLILRLLLLALLALAFAMPILRTRNFGSLLSRTSRDVAIVLDGS
                     YSMNYRLNQQSVWNQATELAAAIVEGLGDNDRFCIYLAGDQVTPICEQMTGKKDEATA
                     RLRALPMPVGSSRLCPATLAALGTLNQEGRRGERELHILSDHQMLPWVSFSAGGEGDA
                     TAGAAGSPDPVGDKWDPSAVQDNTTCFVTLLGTPNPENAAVLNVELEPRLITAQTPCQ
                     VTVNLKRTGTPLETAVHLLVDGKETARRSVMLGGEDEARLRFAFPPLEGGVHTAQVTT
                     PDDSLTEDNAFHFLIHVRERLPALCVGSKENALFLRTALSVSADAEAVSSVEVKNISV
                     AELGGENLSAYSCVFLCNVLPLPGQQISLLERYAAAGGLIVLFPGDGAAVSDYAQWNS
                     LPATPAAITEWPLEKRKQLLTWVLPQHPSIRGLVEGGIAPRVVIKRQLQCETLKEKAQ
                     VVVATSAGVPFLINRPAGRGAVVMLTVSADRSWSDFPLSAFYLPLAHQLIQYAAGVGA
                     RPPYLWATDSLSLNEFLPEATRHSTLSAPNGQPVPLRSVMVEGEAVAYAEGLMAPGIY
                     KLSTPGGGGAQPALAINMLREESDLATVKPADISSILGVSALHVATGKEDLMKQLEDF
                     RIGRSLGEALLWLTLLVAVVEVLYANSLLRKTAKLTDTMKIAPSGRVKEKEA"
     gene            complement(21461..22414)
                     /locus_tag="PLZ74_01270"
     CDS             complement(21461..22414)
                     /locus_tag="PLZ74_01270"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOR96984.1"
                     /translation="MAKKGKAKGTVAAKPSRQAGEEGIRAFLNPEEMHKLNRIVLQSR
                     YVVEGNLAGAHRSPLKGASSEFAEHKAYGMGDDPKHLDWKVLGRTDRYYIKRYEDETN
                     LRVYLILDRSGSMSYGSAAVRKFDFACQLVAALGYVVVKARDSVGLFVYSDKLDMQVE
                     PRNSVNHLHNMLKRLQGVEPASTTRTAETLHQIAESIRKRALVVLVSDLLDDEREVTL
                     ALAHFRKRLHDVIVLHVLDPVEIDLSLKQGFEFEDLETGERITADPRGLAKEYQQAFA
                     KFLEHYRVSCEGMRIDYRLVRTGQSVETYARAYLEERRRLR"
     gene            complement(22460..23146)
                     /locus_tag="PLZ74_01275"
     CDS             complement(22460..23146)
                     /locus_tag="PLZ74_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96985.1"
                     /translation="MKGAVSRRNVAALVLLTAGVAAAAVRSGNEYALSLVEPKAPAVR
                     KSNADEGRGKQFFFRYLEVLEVEKGQTGGAPHMTLKTREPSSGVIVRFVVRKSVSLSV
                     LQQAPATVVGDAVAVTGVVSAADPTAREIVLNPVIVRYKDRLAPKVGKEMLSEVDSSA
                     VVYSFTGGGVAVNVSKRDEDLIANEKEMIAKLGKEGWARYLLTEIAKRDKAERAKRDQ
                     LDIYRKPQGK"
     gene            complement(23289..24602)
                     /locus_tag="PLZ74_01280"
     CDS             complement(23289..24602)
                     /locus_tag="PLZ74_01280"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOR96986.1"
                     /translation="MKRVRLGWVLAVGAVMAVSGQDEDLDWNATPNDVNNLLKSMKEM
                     IDVNYSVQVATLEEVSVDPAKNPILYRSGHYNFSYTEEQRKRIREYCLAGGMIIYNTG
                     LGSAPFYRSAARELAKIFPEQPLQRLTSDHPIFHAYYDVDRVRYCPGVRKSGFVGDEP
                     WFDAVEINCRVVALVSRWCMAVGWEDNVLPEYQAYEADSAKKLGVNIFSYSSAMRAWA
                     KSQAHAIQFIDKPEPSADKVAMVQVIYDGVWKTRHAGLSVILQTFNLKTDVPVKYTVR
                     EMRLKDPAIFNAPLLYLTGHEHFTLNKSEAANLKRYLENGGFLLAEACCGRKGFDLAF
                     RTLLSALFPGTPLRAIPQDSPIFQVPNDVQRVGVTPVLQQALGSAAIRPQLEGLEVNG
                     HVAVIYSPFGLAGGWEMSQSPYARGYDDVSAQRIGQNILMYSVTN"
     gene            complement(24797..25093)
                     /locus_tag="PLZ74_01285"
     CDS             complement(24797..25093)
                     /locus_tag="PLZ74_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96987.1"
                     /translation="MKSEAQKKSIKAGVAMLAATLCMGTVLAGDGSDEPGFVPPPKRS
                     QPPPAPPRSISSAETTDPCCCCPVTPQARTEAKKPPQPPVLITKLKSEGGSSSK"
     gene            complement(25293..26579)
                     /locus_tag="PLZ74_01290"
     CDS             complement(25293..26579)
                     /locus_tag="PLZ74_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HOR96988.1"
                     /translation="MKLESRKKLVFGRCVPLEETVQRLEAVIGSRYDYRLFETSVSER
                     LHWAALYIDDLEFRSMGKGITPLQAKAGALAECAEWAGAKTHAELQGYMVGHQDVIPD
                     AVGIEEMIPHVTLPEKMIGRVKESDIAMFWADGFSLMTGRTAKLPIEFVRRISGPSGM
                     AAGNYREEAIVHAIEEVFERRVHITVLRQRLVMPTIDPDTVTHPVVREQMAFVRQQGI
                     EVHLKDLSFGGVLPCVGAYFWDPNIPESYQFHHFFKVGAGFNLEEALIRVFTEYAQGR
                     MRDEFIDGNPADQERVLKYDLRALRCIPDDGDNYLSAFLFGFVPQRTANFLREGPVVP
                     FQKGEAFDDCLQDIERAKAIFAQLDRECYVIDFTGPEIGFPVVEVVVPGYSDVLPYYP
                     SDSRVLFRPYTRSDILRSYDAAEGTPSAGGASHKGF"
     gene            complement(26664..27455)
                     /locus_tag="PLZ74_01295"
     CDS             complement(26664..27455)
                     /locus_tag="PLZ74_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96989.1"
                     /translation="MGKSIGIMAVIGVCALVCRGQTVQPDERGLWDVWCVGTNSGFVA
                     EESLKACIAFTARTPRDPFVLVMSGFAAWHHLKNGNTEMAVRLFESMQTEKPVTALQK
                     AGDQMARTWLTRLDRERVAHALKKAYLRDIAFPPTLEALKVEGDDAARPPLTDRWGKP
                     WAYRLSSSIQGMAAHRYVLESPGLGADSDLRNALKRPYADGIQLRALRMAAGITDTVE
                     FANPDGVSVRRRLGQEMSGGTLVYLGARLLLLSDGNHWRVLPRPR"
     gene            complement(27607..28608)
                     /locus_tag="PLZ74_01300"
     CDS             complement(27607..28608)
                     /locus_tag="PLZ74_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOR96990.1"
                     /translation="MHNVEFGGALPIPSASGQRCVGRWLTGKTKMDDKKTFTALAVLL
                     VGSAAAEVVPPSHAGQVRGCAADSQTYFAAPAPDTFISHRGESHDAPENTLPAFQMAV
                     ERGFGFECDIYLSKDKRVFTFHDGNLRRTTAGACTLACADANWDDVISKCDVGGWGKW
                     EGSKYSPTRPALLEEVLPLARDGRYIYVEVKTGPEIVPYLKEIFAKQTQATPANTLFI
                     SFNQKSCAELKKQMPEYKVFWLTGAHQKGQDGQKVPVAPEDIVSTLKRIGADGVDIYF
                     DPSVVDQAFVRVVRDAGYEFHVWTIDNLDMSLRAFAAGAQTVTTNCAKKQLDAFRAR"
BASE COUNT         5378 a        10207 c         7843 g         5364 t
ORIGIN      
        1 cacagtctca cagcctttga gattgcgggc tccgcccgca tctcaatacg gtgcgtcggg
       61 cgcaccgcaa aacccaatgt ttacggggct ttgctagaat tccacccgtt caactgccct
      121 ttttaggatt aatgcggcga agcggtcttt tccggcagtt gcgtgtgata ggtgcccgtg
      181 tcctcgtaag tccgcaccgg cacatcccac ccgatctgcc tcagatggtg cgcgaacacc
      241 aaatactcgt gaccgccgtt gggtcgcgta ccccacatcg catgcgccgc acggaaatag
      301 attcccatca tgtcccggcc gtaaaggtgg tgccaggtcg tggagagcat gccttcgagc
      361 ccttcgctga ccacagcctt gccgagcccg cggtatccct cggcattctc ccagggacag
      421 gcgagcacac cgaatccctt ttccttgaaa taccgcagcg tgggccacgt cccctctttg
      481 ttgggcgcac cgtagtacca atcgcaaata atgatgtcgc gcggcaaccc ctccaacagg
      541 cgttcggccc cttccttccc gttcgcgtaa aaccctttcc aacgcggatc gcccgacctg
      601 agcagcatgt cgtgccagat catcatccgg catccgcgcg cggccagcag gtcgcgcaga
      661 tgcgtcagat ggtcccgaac ccgtgcggga tagtccgtcc gacggcaagc cgggcaggtg
      721 cccatgtcat cggcctcatc gcatcccgca tggaaaaatg gaggccgtcc gaaggcctca
      781 tgcagatccg tgacacattc atcaatcacc cgaatggttt cgggattact gaggcaccag
      841 ctccacccca gtggctcgaa gagcggctgc aactccgggt tcagatccag aaccgcatgt
      901 ttgtggcagc tcatgcgcgc ccccgaagca tggcccaacg cattgaactg cggaatcagg
      961 gtcaccccca gcgagtccgc gactttcacc aagtgccgga gcgccttgcg ggtcatcgtt
     1021 ccctccggcc aggccaagcc cggtttacga cccgaaacga acatccccca cggctcgata
     1081 accacatagt tcatcttgta gtaggctgcc agccggatga aacgctccat ctgcaccggc
     1141 gtgttctccg ggaaccagca gaggtggaat ccgcggaagg ccatgaccgg cgcgtcttcg
     1201 atccgcgtcg caggaatggc atactccgcc acttcacgcg ttccccgcaa tggttcggcc
     1261 aactgccgta aggtccgcaa cgcattgcgg accccgccag aatccgcggc ctcgatcaac
     1321 aacgttcggc cggaggcctc gatccggtag gcgtccggct tctgcggcaa cccgtccgca
     1381 tccgcatgac gcaagccgat cttgggcgtg tgcccgaacc actctttgaa aacccgacgc
     1441 gtttccgtca cggcggcttc gccgttcgtc acgcaggcga tcgacacccg caacgctccg
     1501 ttcaacagca cgcgcggtcc gtccgtgagc gtgaccgact gcggcgcggg aaccagccgg
     1561 gccttcagga accgggcctc gccctgctcc aacggttccg cctgcaccca tcccgcgacc
     1621 cagcaaacca gcagcatgcc ccctacgcac catcgattca ttccttcccc ctttccgtat
     1681 gcgtgtcact ttcttctcgt gccgctccgc agccgcacct cacgaccagc gccccgcctg
     1741 caaatccggc tgccggtaca actccgcgca cgttccctcg gcaatgcccg cagactcctc
     1801 atcctccggc tgccgcagtg tccactgccc gcgcggcgaa accgcggtct tgaaagcgga
     1861 ctgttcgatc gcgtggcgct tctccagact gtccatgcag ccgcggacgc atccctgcgc
     1921 accgcagatc gcgaaatacc cgacgtgccc gagaatctga tggtaatagt ccacgatgtt
     1981 gccgtgcggg aactcctccg acgccaccca ggttccggtg gccagagtat aggccatgcg
     2041 gtaggccagt tcctccgaga cacgactctc cggaaccttg aaatccactg tcggaaaatc
     2101 ctggaccaga aacggcgaca cctgccggtt cagaccgtga tgcgtcaacg tgcaacgccc
     2161 catgctgaca tcgccccact cgtaaacctg atcgccgatc tccacccgta ccggcggcct
     2221 ctccccgggt gaggggatcg cgcgtccggg gcaggtccgg acgcatttca tgcagcggtt
     2281 gcagagcgtg cccggtttaa tcagcggatc gggctccagt tccgcatccg tgaggatcgt
     2341 tccgatccgt acgcgcggcc cccacttgcg ggtgaggaac accttggacc atccgatttc
     2401 gccgagcccc gcggccactg cggcgatccg cacgttcagg ttgatctccc gcgcgggccg
     2461 ccccggcttc accggcctgt gcatcccgcc gcgttccggc acggcgggat agagcggcac
     2521 cgcctcccat ccatgatcct cgatgaaaca ggacagcgca tacgtgatcc gcggccggaa
     2581 cagattgttc aaccggttgt acgagtagta ggtgtaattc ggccaatacg tcccctccgt
     2641 aatgccgcgc agggtcccgc ggggaaaagg cgccaccacg gaaatcaccg acttgcactc
     2701 cgggaaaatg ttggccggat gcatctgccg cggcgcgctc cggaaccgtc cgatatccgc
     2761 gacccccacc agacagggcc gccccagcga ggcggcgaac tctttgatct gctgtgatgt
     2821 cagtgtcata tgccgcctat ttggaaaaga tctgacccgt ggcatgcacc gccaccacac
     2881 ccgtgccgcc cgccgcgccg cacccgacat ccgaagcccg caacacacct gcggcgtcac
     2941 gcgtcagcgg ccgtttgagc gcgtccaact gccacggctc gcgtttgcgg aacggctgct
     3001 cgaaacggtt gccgagtttt ccggactgct ccagacgcac cagacactcg cggccgcacg
     3061 ccgccgcgat gcggtccggc tctttgcccc ggccagccgc gagaaacgcg ccgttcgggc
     3121 aggtcgcgca caatccgctc tccacccgtg ccaccggcat ctcctgtccc tgcaccgtga
     3181 tcgtttgaat ctggtccaga tggatcgcgc cgaatggaca ggcctccgca caagcaccgc
     3241 agtcgccgca cagggttttc gcccgcaccg catccccctc cagttcggca tccgtcagaa
     3301 tgaaagcgaa acgctgacgg gtgccgaatt cgggcgtgag cagaaacccg ccgagcccga
     3361 tctctcccac accggcagcc acgaccgcca cacgcggatc gatgtagacg ttcggcgcgg
     3421 gccggtccgg cgcgaccggc acgcccttgg gcatccccgc cccgttgtaa gcgaagatcg
     3481 gcaccgcctc aaaaccctgc tcctcgatcc ggagcgacag gtcatatgag gtctgcgaca
     3541 ggaaatgatc ttccacccac gtataaccga aacactggta ggtactgccg aaattggtcc
     3601 cctcctctac gccgcgcagc gaaccgcggc aaatgcgccg ccccagcacg atcaccgagc
     3661 ggcactccgg aaaaatcgcc gcaggattcc gttccggcgg ctcctccgca aaccgtccga
     3721 ccggtgcaat acccaccaga tccgcaccgc actcgcgggc catcgctttc acttcttctg
     3781 ttttcatcgt tccttcttct cttggcgccg ttgtctgaga aatcaatgca gcagcgcgtt
     3841 ctccggcggc acctgccact gcgccacgcc cgtgtcgcgg tagcgtttga ccggtatgtc
     3901 ccaaccgatc tggcgcacat gccggttaaa ggcggaagag gcattccagc ccgtcccgtc
     3961 atatccccac gtcgcatgcg cgcccgcata gagcatgcgg tacatgtcca ggctgtgcat
     4021 gtagttccac gttgtacaca acatccccgt catgcctttt tcgttgacaa gacgcgcctg
     4081 gcttctgacg ccttccgtgt ttttccacgg gcaggcgagc accgggaatc ctttttcatg
     4141 aaagtactgc atcgtcggcc actgcgccgc atcctcgacc gccatctccg gcaaaaaata
     4201 gcagcgcatc ttctcgacac cgcgctccgg ctcagccaat tgccgcaggg ttttgagcgc
     4261 gttcaacacc ccgccctcac ccgtcgcctc gatcgccagc ccactgcccg aggcgatctt
     4321 gaagcgatag ccgtcttggg ggatcacgag cgatgcatcg gtcttgaagg tcagtttagg
     4381 agaaacgccg aaataggatt tgaacagcct tgaaacccgt tttttcaggc cgcagtcctc
     4441 gtccttgagt gtgaccgtca ccgcaagctc acggtcgacc ttgaccaccg gaccggctgt
     4501 gatcgtcacc tgtttgggat acgggatcat ccgcgacttg ataatctgag cctcgccctt
     4561 ttccatcggc tagccttccg cccagatgcc cggcaccaac cacattgcca tccccaatgc
     4621 ccatatcctt cttccgttca tcgatatcct ccgttccgta ccacagcctt caccgccacc
     4681 gaacaggatt cagcgtgcct gaacaccccg gacttctcgt tccgtttcgt gcttgtcttt
     4741 ttttctaaac gcgctcccaa ccgtacgcac tgaaccgaga aatcctcagc cccgcaactc
     4801 cgcatccatt tcgataccgg cccgcatccc ttccgcgacg cactgcgcca cagacgcgcc
     4861 gccattgatc accccgcccc cggcgaaaaa cccggggagg ctgctgacac gcaggctgcc
     4921 ttcagcggtc ttgaccaacc cctccgcatt gaacgcgcac ccgggcaggg cggacacgag
     4981 agactcctcc agtcccagac ccatggcctc gatgaccagt ccggcttcaa ggagcgtttc
     5041 cggtacaggc ccgccgtctg aatccgattc cagaattttc cggatccgca gccccgtcac
     5101 cttcccgtcc ttcccggtgc gatagccgag cggacgggtc aaagtcagaa aatgcacgcc
     5161 cgccgtcctg aaccacgcat cagccatgtg ccaatgcatc tcggacagcg gaccgtcata
     5221 tacgatcagc agttcccgcg ctcccgcctc cagcgccacc cgcgcgcaat ccatggcgct
     5281 gtcccctccc gccagcagga tcacccgttc ggggacggtt tgacgcgtcc gggttttgat
     5341 ttggcgaagg aaggccagcc ctgcgacaac cccttccgcc gtccccacgg accgctcccc
     5401 ccatacgccc gccgctaaaa aaaccgcatc ggctttccgc ctcaaggaat ccggcgtgac
     5461 ctcgcgaccg aactcgaccc caaaccggat cgtcaggcgc gccgcgcgca gggcgggcat
     5521 caacaccgcc cacgcttctt cgtgcgcacc ggtgaaccgg ctgtcgcgga tcgccagctc
     5581 aggtgtcccg cccaagcggg aggcccgttc gaacagcacc acctgatgcc cccgctccag
     5641 caatgtcacg gcacaggcca cgcccgcagg accgcccccg atgactgcgg ccttccggcc
     5701 gctcggcttt tcagggaggc gcagaccggt caggccgttc gcatgggcat accggtagac
     5761 agcgtgctgg atcgcgtgaa tggggaccgg gacgccgtcc agcgttccgg ccacacagcg
     5821 gccctcgcac aaggtgccga ccgggcagag gctggcacac aatccgggca aaacattact
     5881 ccggcggatg atctcgtacg cggcggcgtc ttccctcttg gcaaacgccc gcagaaacgc
     5941 aggcacatcg atgcgggtcg ggcagcccgc acggcaaggc gcgggttcgc acccccggca
     6001 acgccgcgcc tcttcacgca gacgttccag cgagatcgcg cggcagcggg catactcttc
     6061 gccgtagacg gcgcggtccg tgaccacaca ccccgaccgc cccccgtccc ggaccagctg
     6121 gacgcaggcg ccgcaatcga tacagcatgc ctctaaatcc ggttcgccgt tccggagcac
     6181 cgtccccgcc aaaccgggat ccgccaacgc ggcgcgtccg atcccgatca gcgagccccc
     6241 tccgcgccgc aatacccctg ccgcaacctg aggcaggaat cggcggaacc ggctgaaccc
     6301 ttcggccacc acgggaatct ccggcaccat ctgctgaatg gcctgcgtgc gttccgccag
     6361 gcgcatgaac cgccgcagag gattcacccg ctttttccgg tcgccagccg tccatccaga
     6421 gtccgcgata ttgaaaagcc ccacaccggc ggtggccagc agccgaatcc gttcccgttc
     6481 ttccccaccc tcctttgcag gcgaaagtcg gcaagccacg atcatttcgg gccgcgcgga
     6541 acgcagtctg acggcaacct cccgcagaaa acggctgcgg ttctccagcg atccgccgta
     6601 acggcccggc ctccggaggg cacccagcaa ttccgcatgc agatcgccgt gacaggcttt
     6661 gaggtcgaca ccgtcgaaac cggcctccat cgcaagcaga cccgcagtca cagtggcatc
     6721 ctgaagccga tccaactcct cgtcggagag ccccgtttgc cctgccaaac tctccgggat
     6781 ccccgccggg tccggatcag ggaggatgaa cacgggcgcg ggcacgccct cggggcggca
     6841 ataccggccc gcatgggcca gctgcaggag gatcgcgatc tcatggcccc agcacgtgca
     6901 ggcacactgc cggagttccg cgaccatctc ggcaaaaacc ctgacgttcc ggcgcgtgat
     6961 ccacagttga cggggattgg atcgcgcccg gccctcaacc gccgcagcct ccatccagat
     7021 caccccgaac ccgccctccg cccatctgcg gtaacggcgg cgtgtccaga cgctaggcgc
     7081 gccgtccggc tcggcatcgc acccctccat gggctggaca caaaaccggt tgggcgctat
     7141 gcgtccgccg aaccggagcg gttctgccag cactgcggtt tcgccggaca gcggcagatc
     7201 cagttttaac cggcgggcac tggcgcgcaa cgcctctacc gattggaacg ggaacggctc
     7261 aaaactgttc gcagagggtt tcaccgcact ctccgttgcc tctcattttt ccggggttcc
     7321 gcccgaatcc ggtgcctccc cgcattttcc tgcggatgcc agatacacga caaactcctc
     7381 cgtgccgtcc agtccgagca acgcatccaa ccgggcctga ctgtacccca tggaggaaca
     7441 aaccccagcc cgcagggact ctacggccag atagaggttc tggcagacat gaccggcctc
     7501 gatcgcaatg agtttttgcg cgatgacgcc gtatttccat tcggtccggt acgggaccgc
     7561 gctccagatg aaggtcacag cggcctgacc gataaaggtc tgcccgtaac acgccgccat
     7621 cacccggtca tggatgtccg cgtcttcgcg aaggagcagc aactgatgct ccacgggcag
     7681 ataacggtac aatccgggcg ccaagcccgt aacgcgcgag accgccacgt aactctcaaa
     7741 cgggtgccgc gcccctccgg aagggacagt ccgatagtgg gccgtaacct gtcccgcccc
     7801 gtcccgatcg atccgggcga tcccctgcgt ggcccacagc agatagctga gttcttcgag
     7861 cgaaaacgac gctgcggaga actcacgtcg actccggcgg ttaccgatca cctcgcgcgc
     7921 gctcacatct cccaccgtga aacgctccgg cgccaccaga tccacccgct tggcgtccgc
     7981 gggatacgcc ttctgaatcg gcggcggcgc gagagcgcgg gcagcgtccg tgtcgttccg
     8041 ccaatctgcg gaggttccgc gcaagaaggc gcggcgttgt tcccgtggca gcgcgtcaac
     8101 cccgcggatt ggggccgccg catcgttggg cgaccgcttt tccgcagcaa ccgcaggcaa
     8161 aaacccggcc agtacgcagg cccacattcg caacagccgg cccacccgaa tcggaagacg
     8221 cgccgtctcg aaaacctgac cggtggccgc gcgcgtttgc ggacacctac ttttggtatg
     8281 tttccgcacc gggttctttg acctcctcaa gcagccgctt gacaatctcg acggagttga
     8341 tgccctcgga gtcggcggaa aagttggtca gaatacgatg gcggagcacc ggcatggcgg
     8401 caccgcggac atcgttgcaa ctcacatgga tccgcccctc caacacagcg cgcgcctttg
     8461 ccgccaggat cagaaactga ccggcacgcg gaccggcacc cgtggacacg tactttttcg
     8521 tgcactccgg ggcctgctcg tcaccgggac gggtagcccg caccagactg accgcataac
     8581 ggatcacatg ctgcgaaacc ggaattttcc gcaccacttc ctgcaagtgg atcacggcct
     8641 gtttgtccag cacgacgttc ggctctgacc ccacggccat cgtggtccgg tcgatcaccg
     8701 tgacctcctc ttcgtacgtc gggtaatcca cgaagatgtt gaacatgaag cggtcaagct
     8761 gggcctcggg caacggatag gtcccctcct gttcgatcgg gttctgcgtc gccagcacaa
     8821 agaaaggctt atcgagctta tagatctctt tccccacgct gacctgcttc tcctgcatgg
     8881 cctccagcag tgccgcctgc gtcttcggcg gcgtccggtt gatttcgtcc gccagcagga
     8941 tgttgcagaa aatcggcccc tgcatgaaac ggaactgctt ctgctgagtc tgcgggtccg
     9001 tctccagcac ctcggtgccc gtgatatcgg acggcatcag gtccggcgtg aactggatgc
     9061 gcttaaactt catgtccagc actttggcga gggtgttgac ggtcagggtc ttggcgagcc
     9121 ccggcagacc gatcagcaga gcgtgcccgc ccgcgatgat gcacatcaga atctggttga
     9181 gcacatcctc ctgcccgatg atgaccttgc ccacctcgcg gcgaatggcg tccatggatt
     9241 cgcgcatccg ctcgacccgt ttcagatcct cggcgtcggt cagggatttc gccccgctgt
     9301 cctcacccgc aggttcggaa gcagccgcgg cagcgtccgc ccggaacaga aaggccgaat
     9361 caccgatcgt aagggtatcg ctttcgttca acggcgcgct cacgaccgcc tcgccgttca
     9421 cgaacgtgcc gttggcgctg tccagatcgg ccaccaccca gaccccgttc tccttcgtga
     9481 ttttggcatg atgacgcgag attttcgaat catccaaaag ctggatgtcg cagtcctcct
     9541 cgcgtccgat catgatttcg gccccggtca gcacgatctc ctgaccctgc ccctgccctt
     9601 ttgtcaccaa tagactcgcc ataaaacgtt cctttctttg cccgtgacag gcggattatt
     9661 tttctttcct gagatccagc tcgcctaaat cccggatgcg gttcgtcacc cggatgcggg
     9721 agagttgttt cgaataatga tgggccggtg tgagcagctt cagttccacc gtcacgggat
     9781 acaaatcccg cttttgcacc acctgccagc ccacgcccac atccttcagc cagatcagct
     9841 tgaatgtccg gcgtcccttg ccagcctcca acgctgtgtc atcacccttg accatccccg
     9901 gaccgtcccg gtactgggtc accacgcagc gggcgaaatt gccccgcccc gtggttccgg
     9961 ccatccggtg gatgaccttc ctgttgaaaa acggatccga ggcgttcacg atccgggtga
    10021 tgctctcgcg gtccttgggc gtcagggtgt cgggttcaga cgacaactca agaccctcga
    10081 aaaactcatt ttcataaatg acgaaaaggt tgtacctcgc catcggaaga ttctgaaaca
    10141 gaaactcctg ccggtccccg cccgtcacgg tgccctgata aacgaaacgc ggttcatccg
    10201 gcggcatggc caacacctga aggatcggct gggagggtct gacgatccgc cctttcagtc
    10261 cacccgtggc cgacgggtcc gtggccgtat aaagccggga acgctcttcg gcgccggtta
    10321 ccgaaagcgc gcacgccagc agcacccccc acacgcattg tacaaacacc ccgttcatct
    10381 ccgcttctcc cttctgtccg cttccaacgg ccacgcgcga cggctcattg atcgcctccc
    10441 gcgaaagtcc gtctgcgaat ctcctccgtg tatttttcga tcacctcggc aggctcgttc
    10501 aaatccatca tgcactgctt gttcaactcc atggccgcca ccagaaaatt tgaagcgtcc
    10561 acctgatcgc aataggcgct caagatgaca cgcgcccgct tggaatcctt cagcgcccgg
    10621 tagagcttcg cctcgcagag cagatagtct cccgtcagtt tggagagggg gaagattcgc
    10681 tcccacgcct gcaacgcctg gaacgcctcc agataaaagc cctgttcgat cagaatattg
    10741 acctgctcag aggctgcggc gtcgcggatc gcggagagct tccacgctga cagatcggcg
    10801 ggctgcgcca tcggcgcggg cccggactcc ccgcccttac ccccagaccg ccgcgcgcgc
    10861 gccgcacccg tggccgagag gtccgaaagc cgcttcggcg tgagcgaggc ggccgtggcc
    10921 ttggcacgac tctgcacgtc gccatagcgt tgcgtcgcgg cattgatgtt gcgttcgagg
    10981 aattccagat cgcccatccg gatcttggcc cactcccctg cctcgccgct gtcacgaaga
    11041 agcggggtga tgagtttccg ggcgcccgcc agatcgttga ggtaaaacat catcacctct
    11101 gcggccttca acttcaatac agcggcacga ccactggaaa actcttcaga actcagtttc
    11161 aatgcccacc ggatggcctc ctgcggctta cgcggcccta aggaaaagag gaagatatcc
    11221 tccagcaacc cctgccgttc gggtgtcagc cgtttcttga agaagggcca ccgttgcgtc
    11281 acgatggttt cgaccaacag attggcaccg gagagatcca gcacgcggaa gaggttgttc
    11341 agcatggcgt cgtcccacgc ggcagtcgga tcagcctttt cgggatacgc cttcagcatg
    11401 ctgtaacacg cccgcttgaa tgcgtgccgt gtctcctcat catccagact gctgctgtcg
    11461 aacgtgtagg cgaacagggt tgtggtgcag gtcgcgcgcc gacccgccgc ctcggccgtc
    11521 agtgtgatcc ggctgtggcg ggacccctta aacagccagt gcgtcgtcgg cccgtcggcc
    11581 agcgcgttcg gcgcctcctc gaaactccaa ctgtagcggg tctctttcgg attccctttt
    11641 gtccaggctt tcaacgtata ggcgacccgc ggctcctccc cctcaagcgc gaacagacag
    11701 tcgggcacgg cctcgaaggc ggcggtcaac cccgtctgcg actggatctc gaacagggtg
    11761 cagtcgccgg ttttgaccac atgctcgtct ttgattttgg cggtttcata catcgacgtg
    11821 ccgggatacc gcaaatcgct ggcacgaggc ccgcccaact cctccatggt cgtcttcgac
    11881 gtgcgccagg tgaacatcat gggaccgacg gcctgccctc tggggctgaa tccgaaaagc
    11941 tccacccgat gcagaccggc cgtcaggtta acctcgcccc ccaaaccgcc ctgcttctcg
    12001 tttttcttgg agagcggaac cggtttgccg tcaatcacga tctccatcgc gccggagcgg
    12061 atcatcggcg cgacccatgt gggaccgggg gccgtgacct caatgtgggc cagcagatag
    12121 atggcgttcc cgccgggctt ccactcgcag accgccatcg ggaccgattc gccctgccag
    12181 gttcccgctg accaagcctg attgtgaaaa tgggtcttgg catccaccga acccaaccgg
    12241 cttagtttct gcgccgcggc tttggcgctg gttccgttga cctcacagcg gatggcactg
    12301 ggggtcaacc cgcttgccgg agtccacgtt gcgagttttt tcgcgccccg gacatacaga
    12361 accgcggtct tcccctcttt cggtgccgcg aagaccacgg cgcagccgat ctctttagcg
    12421 tgccacaaca ccgcgcttgt cagcgggttc cccgcctcgt caaacgcgag cgtttcggga
    12481 aaggggcccg gcagactccc gccgtcaggg agtttcacga aaaaaccggc gctggggtgg
    12541 ctgggcgccc gagtcagccc cactacaaag cggatcgtcg cgtcccggac cgcccactcg
    12601 ccgggttccg cggcccggca gcacaacccg gctgccaaga ccgccgggag gagaccccgt
    12661 atcgcgttcc gcatcatcgt tttcatacgc atccctgtct gagcccgcac acgcgggacc
    12721 acacgttaca gttccttgtt gagcttcttg taataatcgg cgttcagctc ccggtacttg
    12781 gcgggggcca gatcctgttc cgcttcgatc acatccgtca gggtcgagac ggccacgccg
    12841 ccgtccagcg tagtcgcgct gccctcgccg agttccgtct tggctttctt caaagcgccg
    12901 atcgccttgc gccgcagttc ggcaacctgt ccaatcggaa cccccttgga aaccgcgtcc
    12961 gaggcctgcc ggatatggtg cgcggccgtt ttcagcgaat cggtgcgcaa ccctttcagg
    13021 gaagcctgcg catacgactg gtcggccttg cgcgccatct gctccatgaa ctgcgcccac
    13081 gagccggctt cggaggcgtc catgcgttcg gtaccgccgc tgtcgcccca gtcgtcgcct
    13141 tttccggaac ccagcttacc gccgcccgtg gccttggttt gcgcctcgcc atccgccacg
    13201 acctgtcccg actgggtcgg atcctgcgtc cggcgcgcct cgatgttgtc gtccccctcg
    13261 gagattgtcc ccgaacccgc ggcgctttca ccgctcgaca tcccctgccg cccgacattc
    13321 gaccgcccgt cctgttcttt gtgatccggc gtcgtgttcc ccgatttacc ctttgcggag
    13381 aaggtggtcg tgtcgccctc ggtgatcaga gcgcccatct ccatgtccgg caacgcctgg
    13441 ttgatggcac cgtcgtcggc ctccctgtcc atcttctcat cgatctccag cagatcgccg
    13501 atgatgtctt ccattgcggt ctgcaggggt gtcagcgcga caccttccgg catctcttcc
    13561 ttgtcaaagg cctcatccgt cactttggtg gtgtcgggct tatcccccag ccacatctcg
    13621 acatcctcaa tcaacttttc ggccttctcc atcgcttcca gatagtgctc gcgcttcatg
    13681 acggccttct ctttaaccgg accgccctcc tgatccttcg tccccgagtc cttttcctga
    13741 gtcacctcct cgaacacgga ataaaggtcc tcgaccagat cgttgccgac attcaggtgg
    13801 gcgaaaatat ccaaatcgcg cgggatctgg agcatcgcat cctcgatgtt cttccggatc
    13861 tcctcggcct cctgctccat gacgtcgaaa tcttccgtgt tccggtcccg gttttcggcg
    13921 attttatcca tctcttcctt caaccgtttt tcggccgcct tgagcttctc cagtttcgcc
    13981 ccggcgatct ggagtgcctc gatcatctct tcgcgcgact ccttctcctc ctgcgcctga
    14041 acctcctcga accgtttgcg cacaatcgtc agcttgtcaa acgcgctccg cgcgtgcggg
    14101 atcgcctcgc gcgccgcgtt ctgctccagt tgttcagcgg acagcagcat gtcgtccgtg
    14161 atcttttgct gtttgcaaaa agcggcgacc ccttcgagaa acgcgctctg cgatttgtcc
    14221 tcgccctgtc ccgccgccgc ctcggccccg caggcctgaa tgaaggccac cacctccgaa
    14281 ctcagcgcgt cctgctcatc caccagcgag gccgcgaccg ccaccccctg agtggcgcac
    14341 tgggaagcgg tctgcaagac cttcgcctgc ccggtgatga tcccgtcgag catcccgatc
    14401 agggaactgg cgctctggtt ctcctgggct tttgccgcgg actccccggc aaaggtcagt
    14461 tgccggagga ttttctcctg catcgtcagg gccttgccaa cccgccgatc tttttccgtt
    14521 atcccgttcg tggccggaac cgaccgcagt gtggggatca cttcatgcag ttcgttcagg
    14581 tagagtttct tgatcgtggg caccaaattc cccaagctct tgccgccgcc ccgctccaac
    14641 agcagccgga ccgtctggcg gatctcctct tgtcgcgtgg cggtctcagc ccactgttcc
    14701 ggggtgctgt tcgccaaagc atcgcgaagc tgcgtgacct tgcggagatt ctcacgttga
    14761 aggaggatga cccgctccag actctccctg ctctgtttgg ccagttcctc ccgcttcttc
    14821 gccgcctgat ccgtctcgtc cagttcaaac accagcggac cggataccgt ggtctggccg
    14881 ttgccctcgc ggttgtcccg ggcctcaacc cgcatcaccc acgtgccggt gtccgttgtt
    14941 ttacggacca cacccttcca cagcgcggaa aactctttgc cccggatctc cgcagcagag
    15001 aaggtcttca gcaccttggg ccgcgcttcg gactcgcctg ttttgggaag ctgcacaatg
    15061 gagatctcat cgaacccgaa gtcatccgag gccgaaaaat ccaccgtcgg agccataccg
    15121 ggcggcaacg gcacgggttt ctgcggatat cgaaccgtta gcgcgggcgg acggtccggc
    15181 aggaggttga accccactgt gctctccgcc ttgtcgcctt ccgcgctctc tgcggtgagg
    15241 ataaaggcgg caccgttcgt gaccacgagc gcgacgctcc cgcccggttt cttcccttgc
    15301 tcaacgaact ccaccggatc accgccccag ccggaaacga ccagcgaacg caacggcgcg
    15361 ttacagcgcg ccgcgagttc aacccgggat ccggccggga tgtcgagggc cgtcgcctgc
    15421 gcatcaaccc gtttcgtcgg caggcgcata taggcgggcg gcgtcacctt cagggtcacg
    15481 gcgcggaatg ccaagggcgg ccgcagggtg atcgtgctcc atcccggcgg atacgcgtcc
    15541 cccgcgcgga agcggtattt caggtcggtg gtgactttgg ggatcacatg ctggaaccgc
    15601 tcctccgcgc ctccccggag cgtacccaac cgataggtct tctgctcgcc gtcggatgga
    15661 cggacatcca gaagcaccgt gtgcccggcc cggccctgca cctcgcacgc cagcgtaagc
    15721 acaccgccct gcaggacggt cgcgtcgccg gggctcacgc ggagaatgcg ggtcaggctg
    15781 accggcgtga gttcagcgaa ggggttcagt acccgcgcca tcgccacggg ccacgcccgc
    15841 cccgcgaacg gatacggcag caacagcagc aacagcgacg cgcccagcgc aagttgcgcc
    15901 cggcgcagca gccgccggtc cttcatcatg cggaaatcca acccgctcca gtgcgggata
    15961 tccattttca catacgccgc gacaaacgag tccttgacac ggaacgcgga aaacagaaga
    16021 aagttgatca gatggttatc gagttgcggg aaggtctgct ccacacggac tgccacggcc
    16081 tcgggagtgt ggcgccgcgc caacgcgcgg aagaccagcc ccgccgtcac gccgaccccc
    16141 gccaccagca cggcccaggc cagcaaccgc ccgttgcggc cgtaccggac cagcagatcg
    16201 acacccgcca gacagaagac cagcgcccca aacaacgcca gcccggccgt cagccggaac
    16261 tccgcctgcc agcggttcag cagtcggccg atcttttcaa gctgtccgat caatacggat
    16321 ggagccatat cccgtctccg tgcatcaggc cgcgctcagg cgcggcacga cctcatttcg
    16381 ttaccttgac cttctccggc aacttgacga tcttcaggtt ggacacgtaa aaattcccgt
    16441 ccctcaaata cgtcgagcct ggcacaaaca tgaagaccgt gatgtaccgg acttttttca
    16501 ggtggctgta cgcgaccggc gagatcgtct cgttggggat ggtgacgcgc atagtcttcc
    16561 acgccgccgc tgtcgccgcg acgtactccg ccttgtacac gcgccgcagg ttctgcagtt
    16621 tcgggggttc gctcggcgaa atgcccggct tgaggttgta ccccagaaac aaaatgtgca
    16681 cctccaaatt attctccacc agaatgtcaa aggtgcactg gtagcggtcc ccaggctcat
    16741 acgggatcag cggcgtctcg accttcgtct catatccgct cggcaccgcc attttcagca
    16801 cccgctcctt cgcccggaac atcggcagga ccgaggcgtt cttggcattg cccgtctgtt
    16861 tcttgatgag ggtccagtca taatcgatat tccagccgtc cagcggccct ttggcggacg
    16921 tgaagtcgct gttgtaacaa tagttggtct ccgcgggtgc cgcgcgcaac gccgacacca
    16981 gaacaacccc gcaaagccac acggcacctt ttccgatccg tttcatctgc ccctcccggt
    17041 cgcatgaccg tctacgtgag attctggaat tttcggagaa tccactccag agcgatcaaa
    17101 gtgattagga taccaagaat cgcccaatgt tgccaatacg tcttatactc gaagacgctc
    17161 tgctcgatgc gcttgggcgt cacggacaac aacgcgcgat ccaactcttc cgcagaatcg
    17221 aaatacgcgc cgccgctgtt cgccgcgatc gcccgcaaca ccgacacctc cacaggacgg
    17281 gggatggact cgggcgagta aggcttcacc gcgaaaaagg ccggctcgga ctccagccgc
    17341 tgcccacccg actccgaaac cgccgccacc gcgtacattc ccgcggtttc gcctttaacc
    17401 ttcacgctga aggtcggcac cgccttgccc tcaagcatct ggtcctccct cgccgtcacg
    17461 gcgaacggaa ccacccgctt gtccgggaag gtgatctccg ctttcaccac cgcgccctcg
    17521 ggcgggacgc cctcgcccac ccaacgggcg gtgacttcaa tctcctcgtt caggaaacag
    17581 gtctcgcggt cgagaaagac ctcccaactc cgcccctcga tctggtcggc cttgggcgaa
    17641 agccatgaga gcaactggtg ccaaaaacgc ggataggggt tggtcttgag cgcgtcagcg
    17701 ctcaactgcc atttccacaa cgaatccgag aacagggcca ccaccttgcc ctgtccgaaa
    17761 tcctgggcaa ggatcatcgg ttgcggaccg ctgtcgctac ggacctccac cagcactcgc
    17821 gccgcggccg agggcacgac cccctgaaag atcgaaagca ccgccgggat tttctgccag
    17881 aactcggcat cccccccaaa ggccgcgtgc gaacgcccct gctcggtcag gctcacggcg
    17941 aactcgccct cccgcgcctt gcctgaaaag gctttggcgg gcagcagtcc tttcaatgcc
    18001 gtcttcgcaa aaccgtccgg tgcccaggct ttgctgcccc caagcagcac cagactcccg
    18061 cccgcctcca caaacttcac cagcgcgcgc gcgcgctcct cccccagttc ctcgccgctc
    18121 agattaccca acaccacaat cttaaactgc gcgagctgcg cctcccccat gtccggcgcg
    18181 acctccccgc tcaccccgta cgtcatgaac ttccccttgg gacccttcag gaaaatcgag
    18241 gggctgacct gcttgctctc gcgcaacacg cgggacagat atttggactc ccaccgcggc
    18301 gggccttcca catacatcag gcggttcttg gcatccaaca cctgcaccac caccgacgat
    18361 tcgttgtcat cgacctgcga ctcgccggac agcgcgggca gggagacccg ataggtgttg
    18421 acgccgatgg ccgcgtgatc cagcttgaac accacttcgc gcgacccgcc gccctcgggg
    18481 atctgcgtct cgatacgctg caacagcacg tcgtccttga aaagctgtac tgcgaagggc
    18541 tgtccccggg ttccctgtcc cgacagcacg gccttgagtt ccgactgcca cccgaccgtg
    18601 acacgccgcg gtgtgctgag cgtctccacg cggatatccg gctcctcctc ccagatcgcc
    18661 tctcgttcca gcggcagggt ataaatcggg aagggccgct tttccaacgc ccactccttg
    18721 aacgcctcgc gcgtgtccag cccgtcgctg agcaacaggc atcccgtcac gtccacgccc
    18781 gcgtaccggc ccacgagttt cttcagcgcg tcgcgcaaca acgtggcctc cccctcaggc
    18841 tcgcgtttga agagttccgg cagcgaagtt ttctgctgaa gatccgcgga aaacggatag
    18901 acctcgacct cgcatttttc agccatacca gccgcccacg gcatctggag caccgtctgc
    18961 gccgtctgcc agcggttggt catccccttg accggggtca tcgtcatgct ccgggactcg
    19021 tcgaccaaca ccagaaagcg ggttctctgc tccaacgttt cgacacgctt ctcgccgggc
    19081 agaagcaggc accaccccag cagcaggaag aaacagaccc gtgacaacag catcacccac
    19141 atcggcccgt cccgcttcac aaacagaccg tagccgaccc cggtggccgc taaagccgcc
    19201 aggacccctg ccgcgataac agcggtcggt gcgagatggt cgtaaatgat cacgcttctt
    19261 tttccttcac tctgccggag ggggcgatct tcatcgtgtc cgtcaacttc gcggtcttcc
    19321 gcaagaggct gttggcgtaa agcacctcca cgacagccac cagcagggtc agccacaaca
    19381 gcgcctcacc cagtgaccgg ccgatgcgga agtcctccaa ctgcttcatc aggtcctctt
    19441 tgccggtcgc cacatgcagc gcggacacgc ccagaatgga ggagatgtcc gcaggtttga
    19501 ccgtggcgag atccgactct tcgcgcaaca tgttgatcgc cagagccggt tgagcgcccc
    19561 cgccgcccgg cgtgctcagc ttatagatcc ccggtgccat cagcccttcg gcgtacgcca
    19621 ccgcctcgcc ctccaccatg acactgcgca acggaaccgg ttggccgttc ggcgcgctaa
    19681 gcgtcgaatg acgtgtggcc tccggcaaga actcgttcag ggagaggctg tccgtagccc
    19741 aaagatacgg cggacgggca cccacccctg cggcgtactg gatcaactga tgcgccagcg
    19801 gcagataaaa ggcggagagg ggaaagtcgc tccacgagcg gtcggccgac acagtgagca
    19861 tcaccacggc cccccgtccc gcagggcggt tgatgagaaa gggcacccct gcggaagtcg
    19921 ccacgaccac ctgagccttt tccttcagcg tctcgcactg gagctggcgt ttgatcacca
    19981 cccgcggcgc gatcccccct tcaacgagcc cccggatgga cggatgctgc ggcagcaccc
    20041 acgtcagcaa ttgtttccgc ttttcaaggg gccactccgt gatcgccgcg ggagttgcgg
    20101 gcaggctgtt ccactgggcg taatccgaca ccgcagcacc gtcgccggga aacagaacga
    20161 tcaggccgcc cgcagccgcg taccgctcca gcaggctgat ctgctgtccg ggcaacggca
    20221 gcacgttaca taaaaacacg caggagtagg cggagaggtt ctcccccccc aattccgcga
    20281 ccgatatgtt tttgacctcc actgaggaga ccgcctctgc gtccgcgctc acgctcaacg
    20341 cggtgcggag gaaaagcgcg ttttctttgg accccacaca taacgccggc aaccgctcgc
    20401 ggacgtgaat cagaaagtgg aacgcattgt cctccgtgag actgtcatcc ggcgtcgtca
    20461 cctgcgccgt atgcacgccg ccctctaacg gcgggaacgc gaaccgaagc cgcgcctcgt
    20521 cctccccgcc cagcatcacg gagcgccgtg ccgtctcctt gccgtcgacc aacagatgca
    20581 ccgctgtctc cagcggcgtg ccggtgcgtt tgagattcac cgtcacctga cagggcgtct
    20641 gcgcggtaat gagccggggc tccaactcca cgttgaggac cgcggcgttt tccggattgg
    20701 gcgtccccag cagcgtaacg aaacaggtgg tgttgtcctg caccgccgag ggatcccact
    20761 tgtccccgac cgggtcaggc gaacctgcgg ccccggcggt cgcgtcgccc tccccgcccg
    20821 cactgaaact gacccacggc agcatctgat gatcggaaag gatgtgcagc tcgcgctcgc
    20881 cccgccgccc ttcctgattc agtgtcccca gagccgccag cgtcgccgga cagaggcgtg
    20941 acgagcccac cggcatcggc agcgcccgaa gacgggctgt cgcctcatct ttcttgccgg
    21001 tcatctgctc gcatatcggc gtcacctggt cgcccgccag atagatgcag aaccggtcgt
    21061 tgtctcctag cccctccacg atcgcggccg ccaactccgt ggcctggttc cacacgctct
    21121 gttggttcag ccgatagttc atgctgtacg agccgtccag cacgatcgcc acatcccgcg
    21181 acgtgcggct cagcagggat ccgaagttcc gagtgcggag gatcggcatg gcgaacgcca
    21241 gagccaacag ggccagcaac agcaggcgca gtatcagcag cagcagattc tccatccgga
    21301 tgcgccgcga cgagcgtttc tgcgccagtt tcagaaaccg gaccgtgctg aacggcaacc
    21361 gctcggtccg gctcgactgg atcagatgca ggatcagcgg cagggttaaa gcacccaacg
    21421 ccgccagaaa cagtggattg agaaaagaaa tcataatggg ctaccgcagc ctccgccgct
    21481 cttcgagata cgcccgcgcg taggtttcca ccgactggcc cgtcctcacc agacggtagt
    21541 cgatccgcat cccttcacag gacacccggt aatgctccag aaacttcgcg aatgcctgct
    21601 gatactcctt tgccaagccg cggggatccg cggtgatccg ctccccggtc tccagatcct
    21661 caaattcgaa cccctgcttc agggaaaggt cgatctccac cggatccagc acatgcagca
    21721 cgatcacgtc atgcagccgt ttccggaaat gggcgagcgc caaggtcacc tcccgctcgt
    21781 catccagcag atccgagacc agcacgacca gcgcacgctt gcggatcgat tcggcgatct
    21841 ggtgtaacgt ctctgccgtg cgtgtggtgg aggccggctc caccccctgc aaccgtttca
    21901 gcatgttgtg cagatggttc accgaattcc gcggctccac ctgcatgtcc agcttgtccg
    21961 aatagacaaa cagcccgacc gaatcacggg ccttgaccac cacatacccc aatgcggcga
    22021 ccaactgaca ggcgaaatcg aacttccgca cggccgcgct accgtaggac atggagccgc
    22081 tccggtccag aatcagataa acccggagat tggtctcgtc ctcgtaccgc ttgatgtaat
    22141 accggtccgt cctccccagc accttccagt ccaaatgctt ggggtcgtcc cccatcccgt
    22201 acgccttatg ctcggcgaac tcggaactgg cgcccttcag gggactccgg tgcgcgcccg
    22261 ccagattccc ctccaccaca taccgggact gcagcacgat ccggttgagc ttgtgcatct
    22321 cctccggatt gaggaaggcc cggatcccct cctccccggc ctgccgggag ggtttcgccg
    22381 ccaccgtccc tttggctttc ccttttttcg ccataccccg cttcacccga tcctttacgc
    22441 gcccgcccgc atgtccgtat cacttgcctt gcggcttccg gtaaatatcc agttggtccc
    22501 gtttcgcccg ttccgccttg tcgcgtttgg cgatctccgt cagcagatac ctcgcccacc
    22561 cctctttgcc gagcttcgcg atcatctcct tctcgttggc gatcaggtcc tcgtcgcgct
    22621 tggagacgtt caccgccacc ccgcccccgg tgaacgagta caccaccgcc gaggagtcca
    22681 cctcggaaag catctcctta ccgactttcg gcgccagacg gtccttatac cgcacgatca
    22741 ccggattcag cacgatctcg cgcgccgtcg ggtcagcggc ggacaccaca ccggtcaccg
    22801 ccaccgcgtc ccccacaaca gtggcggggg cctgctgcag caccgaaagg gagacgctct
    22861 tgcgcaccac aaaccggacg atcacgccgg aactcggctc gcgggtcttg agcgtcatgt
    22921 ggggagcccc cccggtctgc cccttctcca cctccagcac ctccagataa cggaagaaaa
    22981 actgcttgcc ccggccctcg tcggcattgc tcttacgcac ggcgggtgcc ttgggctcca
    23041 ccagactcag cgcgtactcg ttaccgctgc gcaccgccgc ggcagcgacg cccgccgtca
    23101 gcaacaccag tgccgcgaca tttctccgac tcaccgcacc cttcattcca agctccgttc
    23161 ccttacgttg cctaacaact tcatgtccga tccgcagatt cccgcaggtt tggattcgat
    23221 ctgtgccatc tgtgtaatct gtggatcacg ttaccgcatc gtttgtttcc gctccgcagg
    23281 cggcagactt aattggtcac cgaatacatc aggatgttct gtccgatgcg ctgggcgctg
    23341 acatcgtcat accctctggc gtacgggctc tgcgacatct cccacccccc ggccaacccg
    23401 aacgggctgt aaatcaccgc gacatgcccg ttcacctcca gaccctcgag ttgcggccgg
    23461 atggccgccg aacccagagc ctgctgcaga accggcgtca ccccgacccg ctgcacatcg
    23521 ttgggcacct gaaagatcgg gctgtcttgg ggaatcgcgc gcaggggcgt gcccggaaac
    23581 agcgccgaca gaagagtgcg gaaagccagg tcgaacccct tgcgtccgca acaggcctcg
    23641 gccagcaaga acccgccgtt ctccaaatac ctcttcaggt tggccgcctc gcttttgttc
    23701 agcgtgaagt gctcgtgccc ggtcagatac agcagcggcg cgttgaaaat cgccggatcc
    23761 ttgagccgca tctcccggac cgtgtatttc accggcacgt cggtcttcag attgaaggtc
    23821 tgcagaatca ccgaaagccc ggcgtgccgc gttttccaga cgccgtcata aatcacctgc
    23881 accatggcga ccttgtccgc gctcggttcc ggcttgtcga tgaactggat ggcgtgggcc
    23941 tggcttttcg cccaggcgcg catcgccgaa gagtaggaaa agatgttcac gccgagcttt
    24001 ttggcggaat ccgcctcata ggcctgatac tccggcagga cattgtcctc ccagcctacc
    24061 gccatgcacc agcgcgacac cagcgccacc acccggcagt tgatctccac cgcgtcgaac
    24121 caaggctcat cgccgacaaa gccggacttc ctcacgccgg ggcaataccg gacacggtcc
    24181 acatcgtagt aggcgtggaa gatcggatga tccgacgtca ggcgctggag cggctgctcc
    24241 ggaaagatct tcgccaactc ccgcgccgcg gagcggtaaa agggtgccga cccgagcccc
    24301 gtgttgtaaa tgatcatgcc tcccgcgaga cagtactcac gaatgcgttt gcgctgttcc
    24361 tcagtgtacg agaaattgta atgtccggaa cggtagagga tggggttctt ggcgggatcc
    24421 accgacacct cctccagcgt cgcgacctgc acgctgtagt tgacgtcgat catctccttc
    24481 atgctcttga ggagattgtt cacgtcattc ggcgtggcgt tccaatccag atcctcgtcc
    24541 tgccccgaaa ccgccatcac ggccccgacc gccagcaccc accccaaccg aacccgcttc
    24601 atatccctga tcctttcatc cggccgcgcc ccggccagat aacggacagg ccgccaacag
    24661 gatgggacat cactgtcgca aatcctgccg acggccagac cagtatcccg caacggactg
    24721 aagccgcata accccgggtg tgtcaatctc ccgtgcggtc attgcatacc tctgccgccg
    24781 ccctccgaac cgacactcac tttgaactcg aaccgccctc gctcttcagc ttcgtgatca
    24841 gcacgggcgg ttgcggcggt ttcttggcct cagtccgggc ctgcggcgtg accgggcagc
    24901 aacagcacgg gtccgtggtc tccgccgaag atatcgaccg cggcggcgcg ggcggcggct
    24961 gtgaccgctt gggcggcggc acaaaaccag gctcgtccga accgtcaccg gccagaaccg
    25021 ttcccatgca cagcgtcgcg gcaagcatgg ccacaccggc ttttatactc ttcttctggg
    25081 cttcgctttt catgccgtcc cctcctcaac aggcgtcacg ccaaaaaccg tatagcaaca
    25141 ccttaacacg gccccccggc actgtcaacc cgacccggca aatttttcgt aagctgattt
    25201 caccccgctc ctgtcggcac cgaaagacgc atttccgctc accgcatcat cacctcgaat
    25261 ccgccgtcac gggtcacggc gaccgcaccc gatcagaaac ccttgtgcga ggccccgccc
    25321 gccgagggcg tcccctctgc cgcgtcgtag gaacgcagga tatcgctacg ggtgtagggc
    25381 cggaacagca cccggctgtc agacggataa tacggcagca cgtccgaata tccgggcacc
    25441 accacctcca ccaccgggaa cccgatttcc ggccccgtga aatcgatcac gtaacactcc
    25501 cggtccagtt gggcgaagat cgctttcgcc cgctcaatat cctgcaggca gtcgtcaaac
    25561 gcctcgcctt tctgaaaggg cacgaccggc ccctcccgta aaaaattcgc cgtgcgctgc
    25621 ggcacaaacc cgaacaggaa ggccgagaga tagttgtcgc cgtcatccgg aatacagcgg
    25681 agggcgcgca ggtcgtattt cagcacccgc tcctgatccg ccggattccc gtcgatgaac
    25741 tcgtcccgca tgcggccctg cgcatactcg gtgaagaccc ggatcagggc ctcctccaga
    25801 ttgaaccccg cgcccacttt gaagaaatga tggaactgat aactctccgg aatgttcggg
    25861 tcccaaaagt aggcccccac acagggcagc acaccgccga aagacaaatc cttgagatgg
    25921 acctcaatcc cctgctgccg gacaaacgcc atctgctccc gcaccacggg atgcgtgacc
    25981 gtgtccggat cgatcgtggg cataaccaga cgctgccgaa gaaccgtgat gtgcacccgc
    26041 cgctcgaaaa cttcctcgat cgcgtgaaca atcgcctcct cgcggtaatt gcccgccgcc
    26101 atgccgctgg gcccgctgat ccgccgcacg aactcgatcg ggagtttggc tgtcctgccg
    26161 gtcatcagcg agaacccgtc cgcccagaac atggcgatgt ccgattcttt gacgcgcccg
    26221 atcatcttct ccggaagcgt cacatgcggg atcatctcct cgatccccac cgcatcgggg
    26281 atcacgtcct gatgcccgac catatacccc tgcagctccg catgcgtctt ggcacccgcc
    26341 cactccgcgc actcggccag agctccggcc ttggcctgca acggcgtgat ccccttcccc
    26401 atggagcgga actccagatc gtcgatgtac agcgccgccc aatggagacg ctcggagaca
    26461 ctcgtctcga acagccggta gtcataccga ctaccgatca cggcctccag acgctgaacc
    26521 gtctcttcca gaggcacgca ccgcccgaac accagttttt tgcggctttc caatttcata
    26581 cggctctccc tgtcctcgca aatgcgggcg gaacccgcac cccaattccc tctcgcacaa
    26641 aggcacgaga gcacggcccc cgctcaccgg ggtctgggca gaacacgcca atggttcccg
    26701 tccgacaaca acagcaggcg ggcgcccaga tacacgagcg tcccgccgct catctcctga
    26761 cccagcctgc gccggacaga caccccatcg gggttcgcga actcaaccgt atccgtgata
    26821 ccggccgcca tccgcagagc ccgcaactgt atcccgtcgg catacggacg cttcagcgcg
    26881 ttccgcagat cggaatccgc gcccaacccc ggcgactcca gcacataccg gtgcgccgcc
    26941 attccttgga tcgaactcga aagccgatag gcccacggtt tgccccaacg gtcggtcaga
    27001 ggcggccggg ccgcatcatc cccctccact ttcagggcct ccagcgtggg agggaacgcg
    27061 atgtcccgca gataggcctt cttcaacgcg tgcgcgaccc gctcccggtc caaccgtgtc
    27121 agccaagtcc gcgccatctg atccccggcc ttttggagcg ccgtgacggg cttctccgtc
    27181 tgcatcgact cgaaaagccg gaccgccatc tccgtgttgc cgttcttgag atgatgccac
    27241 gccgcgaaac ccgacatcac caaaacgaac ggatcgcgcg gcgtgcgagc cgtgaacgcg
    27301 atgcaggcct tcaggctctc ttccgccaca aacccgctgt tcgtgcccac acaccacacg
    27361 tcccaaagcc cccgctcgtc cggttggacg gtctgccccc ggcaaaccaa tgcgcagact
    27421 ccgataaccg ccataatccc gatgcttttg cccatccgtt tgtctccgcc gcgttccgct
    27481 tcccaccatc cgaacatctg aactatatgc taacacaggc gcagcccgca accccaaaac
    27541 tctttgcctg taaaaacact ccccctcccc gcgttcccga ctgctgaaaa agaagctcgt
    27601 gcgtcatcaa cgcgcacgga acgcgtcgag ttgcttcttg gcgcagttgg tggttaccgt
    27661 ctgcgcaccg gcggcaaacg cccgaagcga catgtcgaga ttgtcgatcg tccacacgtg
    27721 gaattcgtaa cccgcatccc gaacgaccct gacaaacgcc tggtcaacca cggaaggatc
    27781 gaagtaaatg tcaacaccat cggccccgat gcgcttcagc gtggagacga tgtcttcggg
    27841 cgcaacgggc accttctgtc cgtcctgccc cttctggtgg gcacccgtga gccaaaaaac
    27901 tttgtactcg ggcatctgct tcttaagttc ggcgcaactc ttctggttga aactgatgaa
    27961 gagcgtgttc gcgggcgtgg cttgcgtctg cttcgcgaaa atctctttga gatacggcac
    28021 gatctcgggc cccgtcttca cctccacgta gatgtaacgg ccgtcacggg caagcggaag
    28081 gacttcctcg agcagcgcgg ggcgcgtggg cgagtatttt gaaccctccc attttcccca
    28141 acctcccacg tcgcatttcg agatgacatc gtcccaattt gcgtcggcgc acgcaagcgt
    28201 gcaggcaccg gccgtggtgc ggcggagatt gccgtcgtgg aacgtgaaca cgcgtttgtc
    28261 tttcgagaga tagatgtcgc actcgaagcc gaacccgcgt tccacggcca tctgaaaggc
    28321 cgggagcgtg ttttcgggcg catcgtgcga ttcgccgcgg tgagagatga aggtgtcggg
    28381 ggcaggggcg gcgaaatacg tctgactgtc agccgcgcat ccccggactt gaccagcgtg
    28441 ggacggcggg acaacttccg ccgcagcact ccctacgagc aagaccgcca gtgcggtgaa
    28501 tgtcttttta tcgtccattt tcgttttccc cgtcagccag cggccaacgc aacgctggcc
    28561 gctggctgac gggatcggca aagcacctcc gaactcgaca ttatgcatgg tctctccttt
    28621 tgaacttaaa atcacatcat gattgggttg caggctcaca cctgttcagt acgcagggcc
    28681 gggtcgagca ggtcgcgcaa ggcgtcggcc agatggttga acgccagcac caaggcgaag
    28741 atcgcgagcg tcacaaaagt catctcccac cacacgccct gccacaggcg ca
//
LOCUS       DAOSRB010000011        28266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1853_length_28266_cov_7.104959, whole genome shotgun sequence.
ACCESSION   DAOSRB010000011 DAOSRB010000000
VERSION     DAOSRB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 28266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28266
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1853_length_28266_cov_7.104959"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="PLZ74_01305"
     CDS             <1..1681
                     /locus_tag="PLZ74_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOR96991.1"
                     /translation="RMTREVYEGRVFAYQIKPHAEGAFRAGPVRIKAGDKIITNPGPL
                     VQVKGIEAQESVLVSLRASSTSVLVEEPLTLTLAVAVTELPEPYAAENEPLHPNRLPH
                     LSAEFLELRQNTAGLKGPDLNQILNGLIDQSGQQPAFAINEYTTQGMGSGFGRLFDPD
                     PFRPRPIRFRLPHTRETINGRKYRVYTLTLNYTPTQEGEFTFGPVLFKGEVIVDVSAD
                     RKAITRDIYTIGPAVTVRVVPPPDEGRPETFIGAVGRSMQVSAALDATVCKVGDPLTL
                     TLEISGAISVSNLRTPILNLQPELTRDFRIYDENVKADTLPNGKRFKYRVRPTREGTL
                     EFPPIRLTYYDTEAREYRTLTTAPIPLQARPTTQIATPDPDRTDVGLTGTLESHTRPL
                     PAGITLDTQGTRPDPLLPPGRVILLLMTAGPALCLLAFSASPLAACAAAVRTRRRHAG
                     ALRRALAALGRTHTPDRAARTVRRYLTDRLDVAGGALTPDEIADLLQQRGVPVNSATA
                     CRDLLARLDEAIYRPDAPIPDTTLHRDLIALLPEIDAALAETCRVPTKDEL"
     gene            1681..2400
                     /locus_tag="PLZ74_01310"
     CDS             1681..2400
                     /locus_tag="PLZ74_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96992.1"
                     /translation="MSAFFNIILARADAVSGMRRRALVTISCSLFACHGVFALPGDDT
                     ARVFIWEQANAQIAVATTPEAYQKAAETYNRLVADNVCNGPLFLNLGNALVMAGDGIN
                     AAAAFSRAERHLGTTPETRQGLAAAFALQSGRASNDLPWDRIAFVWHYAFPCRIRAYA
                     ALCGWCLLWFGVLCLIRGRHRDRHTFLRSLAETCTLTGGLLATVFAASVLITLAQERH
                     DATTWGARVFLASTPETEGSP"
     gene            2397..3731
                     /locus_tag="PLZ74_01315"
     CDS             2397..3731
                     /locus_tag="PLZ74_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOR96993.1"
                     /translation="MTAPCTGRRIRHMKTSLRTLAATVVLLFGTGLPLHAGELVSGRL
                     YTVPETIYVNQAFEIYFELEVTFGSEVEDLRIRDFPNQPDLLTVGRLVSSSRNRTSRD
                     GKTLDTLQYKATARCHKPFHQTFTPLVQCMLVERRNTGFFSHWQSFPKQMRLDPFTLA
                     VRDLPDQGRPEAFSGAIGTFRLTGRLSQTEAHPGDIITLTLNLKGKGWLGEQTVMPAP
                     AVGPMFKRYPVKELLREDAHLRTEQIFIPQTTNATEIAAVRFHYFNPATGQYEASVAG
                     PFRLTLRDAPAEPVAQTIRMIDTVRPTTASEPPQTISIRQVNRSVRHALPLLIGGAGA
                     LAAFFIMFLLGAGRRRIAIPLALLALGVSLGAAYRIHIRADAATRNLTEQVDVRLAPA
                     RAAFQLFSLPAGTEVIPLETSASWVRIDADGRRGWIRAEALGDPTTVPKGAP"
     gene            3728..4459
                     /locus_tag="PLZ74_01320"
     CDS             3728..4459
                     /locus_tag="PLZ74_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOR96994.1"
                     /translation="MKERLDTLMVRAGLAESKERAQRLILAGEVFAAGQLATKAGHKY
                     PVDIELTVKAQPRFVSRGGEKLEGAFCAFPEFNVTGRVCLDVGASTGGFTDCLLQHGA
                     ARVIALDVGKGQLHWSLRQDPRVQVMESFNARYLRLSDLPEQPQIGVTDVSFISLRHI
                     LPPMSDVLPSGGEIISLIKPQFEAGRENVPGGVVRDPAVREAVVESIRAFGTELLHLN
                     WLGCVASPLRGPEGNVEYLAWWRKP"
     gene            complement(4590..6257)
                     /locus_tag="PLZ74_01325"
     CDS             complement(4590..6257)
                     /locus_tag="PLZ74_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOR96995.1"
                     /translation="MRLTRLMMIRPYRNAKRMRQIFVVFARCGWACLKAKIQARRSVR
                     AGGAPADLNARIGENLRKALESLGPTFVKLGQILSTRRDILPDGIALELARLQDNVAA
                     IPAQDAICVVETEYGCPLRDVFSEFSTVPIGAASLSQVHEARLASTGESVVVKIQRPG
                     IQTIVATDIEILTRFLPLLERYVEEFRRLNLRSMVNEFHKTILMEMDFHREMHNGLKF
                     KTIFAGDPTVRIPVMYKDLCTDKLLVLEKLDGVKIDQLDELVRQGVDRDTLSKHLVSL
                     MAKQIFRHGLFNADVHAGNIIVLPDHVLGLFDFGMVRTIDPETRQLMTDMFVAVMTKD
                     AVRLSKLLAAYAEPGSQVDVKQLARELHELIVFYYDLPMEDLRLDVLLETGINLLATH
                     KLIIPQQLIMLCRTTVMTESIVLMLSPRINFVKALAPYVREAIRSRVTFSNLSGGISQ
                     FAMDSADTIKALPTAIGTIMTNLSEGKQVVAYRHEGLEKHVRQVSRMTAQLALSIMGG
                     ALFLGSALILALQPDGGGTLRSTLGTAGLTVAVLLFVGVSLSMLRKK"
     gene            complement(6381..6698)
                     /locus_tag="PLZ74_01330"
     CDS             complement(6381..6698)
                     /locus_tag="PLZ74_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR96996.1"
                     /translation="MKKEGVCAVEIVLGLAVTGQKKLVAAVESLVKKGELSKQQSQAF
                     LREAQSEGAACKKKVGDQFRDSAKKVLGELGLVTADDLAKIHKEISGLKAAMKAPRAA
                     TKA"
     gene            7794..7867
                     /locus_tag="PLZ74_01335"
     tRNA            7794..7867
                     /locus_tag="PLZ74_01335"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7826..7828,aa:Gln,seq:ttg)
     gene            7896..8840
                     /locus_tag="PLZ74_01340"
     CDS             7896..8840
                     /locus_tag="PLZ74_01340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOR96997.1"
                     /translation="MIVFSGTANLPLAERMMAYLGSRLGDINIRRFPDGEIHVKIVDN
                     VRGRDVFIVQPICTQPNEMLMELLIMIDAARRGSAARITAVLPYYGYARQDRKDQPRV
                     PITAKLVANLLVAAGANRVLTMDLHAQQIQGFFDIPVDHLHAAPVMVKYLREIELKKP
                     LVVSPDTGGAKAAYAYSQMLGTGLAIVAKQRLGDAEVDAFSVVGDVKGCDVIMVDDMT
                     TTCGTLCAAAKILKEQGAASIRAAVTHMPLTDKGVQRLQADTGLTELVVTDTVPLREG
                     LDSAAIPVKLTVLSVADLLGEAIRRIHNDQSVSSLFRY"
     gene            8871..9551
                     /locus_tag="PLZ74_01345"
     CDS             8871..9551
                     /locus_tag="PLZ74_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOR96998.1"
                     /translation="MANEKIRMKAELRTEQGSGAAGRLRRAGSVPGAVNRIEGGTTLV
                     KIDAHAFKTMLRHHGREQMLVTLEMDDKEVSALMREVQYDAITGYPIHVDFGEVSLTE
                     KIRVALPLQLTGEPEGVKMGGGVLQQMLRTIDVECLPTDLVEHFEVDVSGIKLGQSLF
                     VRDLKLGDAYTLFTSRDGVVATVAAPEEEEEATAATPEAAAAATPEVITKGKKEEDGE
                     ASAPAAKK"
     gene            9576..10169
                     /gene="pth"
                     /locus_tag="PLZ74_01350"
     CDS             9576..10169
                     /gene="pth"
                     /locus_tag="PLZ74_01350"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOR96999.1"
                     /translation="MIAGLGNPGEMYANTPHNVGFDVVDLLAERLGCGWKRSARFHAF
                     VSRTRCAEEPILLVKPQTFMNLSGTSVVPLLRYYGGEPDDLIVVLDDADLPLGMLRIR
                     AAGGTGGHRGLTSVIEALGSDAFTRVRLGVGREGRGSLADHVLGKFDEARQEKARAVV
                     EAAADAVQCLTEKGLNEAMNRYNGWRADAPLPPDDRK"
     gene            10182..10652
                     /gene="rpsF"
                     /locus_tag="PLZ74_01355"
     CDS             10182..10652
                     /gene="rpsF"
                     /locus_tag="PLZ74_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOR97000.1"
                     /translation="MMKKYEGLYIFAGSAKDDVLDKQVEKVLGEITRLSGNVISTDVL
                     GKRTFARPMNKRENGVYVKIRFELDPQHVTDLKNRYRLLEDLFRVQILVVDERRESVL
                     AQQEEAHKARAAARAAAAEAEAEAAAEAEAAAEAAAEAAEEEEEEVESQDERDA"
     gene            10684..11145
                     /gene="ssb"
                     /locus_tag="PLZ74_01360"
     CDS             10684..11145
                     /gene="ssb"
                     /locus_tag="PLZ74_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOR97001.1"
                     /translation="MASFNKVILMGNLTRDPELRQTPTGAKVAELRLAVSETWRDKSG
                     QSKEVTCFVDVVVWEKLAELCHRYLSKGRPVLVEGRLQLDEWKNQQGETRSKLRVRAD
                     TVKFVGPPPARSGETPTAAQPAAPDASVPVADRTDYNVPDDLTEDDEHIPF"
     gene            11251..11436
                     /gene="rpsR"
                     /locus_tag="PLZ74_01365"
                     /pseudo
     CDS             11251..11436
                     /gene="rpsR"
                     /locus_tag="PLZ74_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            11458..11910
                     /gene="rplI"
                     /locus_tag="PLZ74_01370"
     CDS             11458..11910
                     /gene="rplI"
                     /locus_tag="PLZ74_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOR97002.1"
                     /translation="MSTELLLLSDIPDLGQAGDVVKVAPGYARNYLLPKDLAAPVSPQ
                     ALRRLEKLRKEREEQTRLRLAEANAKAAKLTNASVTLRAKTVDGTRLYGSISAADIAA
                     AIETENKVTVDKSQIELPEPIKETGTFDISIVLHDDVKPVIKVWVVED"
     gene            12053..13456
                     /gene="dnaB"
                     /locus_tag="PLZ74_01375"
     CDS             12053..13456
                     /gene="dnaB"
                     /locus_tag="PLZ74_01375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOR97003.1"
                     /translation="MEAERGVLGSILLDAATDNRVLDLCIESGITADSFFSLPNRLLF
                     ETLSEMARASQIIDPLTLTERLRALNRLEEIGGAVAIQTLIDETPTAAHAEYYINILR
                     QKHLLRAVITCARDAERRCFDEAISADLILSEVEQKFLSIADRRDGAGLTWPQAITNT
                     MGHIDRLFSLGPGAVAGLPTGLANLDRKLKGLRNGEMIVLAARPSMGKTSLAMNIAER
                     VALGKDMNGRAMKGDHNRPHPVAVFSLEMSTESLAMRMLCGLAEVPSFKIDQGLVNPK
                     KINRQLTDAASKLAKAPIYVDDTGGLDVMDMRARARRMKKRYKVELIVIDYLQLCNCR
                     EFARQGRQIETSQISGQIKAMAKELNLPVIVLSQLSRAPEQRGDKTNKPKLSDLRDSG
                     AIEQDADVVLLLRRPCKASGDPEFDDKLLAIVDVAKHRNGPTGEVRLNFEDTLTRFSD
                     RTDQITEDDRFEPDPDS"
     gene            complement(13554..14936)
                     /locus_tag="PLZ74_01380"
     CDS             complement(13554..14936)
                     /locus_tag="PLZ74_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOR97004.1"
                     /translation="MGGNGVQVRFRAGDIPRAEAARALALDLNRAGYETFFAGGCVRD
                     ALLGRPVQDIDLATAATPDQVEALFPGETVAVGKAFGVVVARRNGFSFDVATFRTDGP
                     YRDGRHPEQIAFTNAEGDALRRDFTVNGLFCDPATGDVTDYVGGLTDLQAQVIRAIGD
                     PAARFREDRLRMLRAVRFAAVLEFTVEPRTREALEVWAPRITEVSPERVAHELNRILC
                     EAPLPSTALNLMRETGLLSPILPEVARLYGTAQPPRFHPEGDVWTHTCLMLDALPPPR
                     DLTLAWSVLLHDLGKPMTCREETDPATGAVRIRFPCHAPVGAAMAEELLTRLRQPSAR
                     IAEVRALVAGHMRGVESGKMRRATLRRFMGEPTFPRMLELMRLDIQHSNGDLTPWRQL
                     VEAYAAFVSEPVLPEPLVRGRDLIAWGVPSGPQVGKLLRELYDAQLEGQLATLEQARE
                     MVCRRRAAPR"
     gene            complement(14936..18781)
                     /locus_tag="PLZ74_01385"
     CDS             complement(14936..18781)
                     /locus_tag="PLZ74_01385"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOR97005.1"
                     /translation="MKKTGLSNQPPRYTAGQANAARTVRRLAGCLCLAGALSAAGLEV
                     WSLDDLQKVGSGIDGWEPDAVYTLMTNLDASATADWNGGAGFVPIGRDAEWGFTGVFN
                     GNGHRITGLTLNRPGESDVGLFGRIGTGGVVSNLCLTGITVAGLVDVGGVAGYLSEGL
                     IRECSVSGDVVGLETVGGVAGFVDLGGIERSSASGSVVADVGAGGLVGYLYEGGTIGE
                     SYAIARVSGTEDTGGLVGSGSGFNSVVSGAYWDTQASGQSSSWGGGTGKTSAEMRQAV
                     TFSGWDFSQVWGIVESVSYPYLRGLAVNGNEFVLTVASRGPGNVFVTPLLSSYTPGTN
                     VTVTAQPVAGVSEFVRWSGGVAQPTNPVTQVTMAGHVSLTAIFRSARGIGSVQELQRI
                     GRDPDYTLNGRYWLTGMLYAGETAGWPDGFGPIGSMTTPFTGSFDGRSNVIARLKVDR
                     PEEDYAGLFGVLGPGAVIRNLGLTYAEVCGNGGLGALVGYNAGGTVERCFAEAQVNGT
                     YNGIGGLIGENREGTVNRAYAVGSVAGQSNVGGLIGLHADGGSVTQTYAACVVTGTSS
                     GVGGLIGWGDGITAGSFWDTEVSGQSGSAGGVPQTTSAMRQAVTYSGWDFIGDWQIVE
                     GEGYPRLRGFARACTVTFDALGGTVEPATAVVDLGGVYGDLPVPERTGYVWEGWRSGP
                     GGAGTPVTEDTVMRTVTNHTVYAHWRADALIVVGGASDAVPIGFTNAVQVSTNHCGDG
                     GTSVCLGGKGIIADGQMAGIEWSVTGPGLLSFCWRTSSEADWDVLRFYEAGTGSIHQI
                     SGIMNNWEEMSILVEGGQETPHLFRWEYEKDPQGDYVGQDCGWVDALRWVPFHPLTVI
                     HGSGGGAYSNGTVVAIQADSPPAYHVFDRWTGDTNSVADVLAASTTVTMSGTGMVLAA
                     SYQATGYPLTVIHGSGSGVYPYGSQVVIGAEVPEGKQFDCWTGDVATVADRFAPTTTV
                     TTTDSALCVAATYRVPLTVVCGSGSGWYAEGSAVMVAADPAPLYQEFAGWNGDATSAL
                     ANATAPSTVLTMPSQAVTLIASYRDSVARLLGCYGPAFTCEGVVDGLTGDAMSGAPSG
                     TPAVKLGGPGVVPDNGFTAFELQVWGSGTVSFWWRVSSEPGADFLRFLVDGTPVMEIS
                     GTKSPWTAVTNTVSGTGTWHTLRWEYRKNGAEASSSDAGWVDDVIWTGDVPQPWLTPD
                     ILLFAVTNDAFCIDFLGERGIPYQVQTNALLRTDGWGGMSVAPVRDGETNGVHRFRAG
                     MAMPGLADTLFFRIRAGGE"
     gene            18930..19649
                     /locus_tag="PLZ74_01390"
     CDS             18930..19649
                     /locus_tag="PLZ74_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOR97006.1"
                     /translation="MPHRTLSLQQAARHIRIPERELFHLVQRGEIPFLRHGDEVVFEH
                     RRLDDWAQRRILGLPTKSLTQHHRETTAERTPHSKDDALVARLFRPEWIDPALPSRTK
                     PAVVRDMVALAVSTGLLYDDAAFQREVEEREAACSTAIGGGAALLHARYHDPFWASDS
                     FIVLGKTQQNIFFGAQDGGETDLFFLICCTDDALHLHALARLCMLAHGSLLLSDLRAT
                     DRADEMHQILLRAESELLRTL"
     gene            complement(19656..21638)
                     /locus_tag="PLZ74_01395"
     CDS             complement(19656..21638)
                     /locus_tag="PLZ74_01395"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOR97007.1"
                     /translation="MTPYGFRPHPQSHDRHLLLACVAVLLSAVIHVVLMVYFSDWTIS
                     GISSLRERGREWFTGDRVPPMRVETMRADPMYIGRKKPGERDTPSRGPIEVSEQVEAL
                     SQSAAPVLTAPPPIPREALQPGVPALKEAASPQIDTTPWMPRQEIAQIYDRTVVDEVA
                     TLPRREIPMIERVPDAPDIVPSIDLVGRRFGNDPEPPKPFEAAEVFDTEISAGTFKVP
                     APEAPATPAPVSTAATESRFAMQPGEKAGKSPTTGSAADTGSATAGPDGASTSEQQKR
                     IERELAAVSQQIAATEVSEEERKAREAQAQIAALQELADYQPIDDLLAVGLETYRDPA
                     DPGRIYFRIGIEPRRDRTVPTLDKDIVWVQDVSASMSEERMVFCRRAMTAALETMNPG
                     DRFNVVAFRDTTEMCFPDWVPVTQAHLKKASTFVAAMRAFGQTDVFGSLRTLMKLPRD
                     PKRPMIAFIVTDGKPTFGMTESAKIIGEFSKLNQGVVSVNMYGTQSKANVYLLDMLTY
                     GNRGGSVVLTGNRWDIPDSMAGAYTGLRYPVMSDIQVTFDSVSQSEVYPKRTTPLYKD
                     RQIQLHGVCPDTTEELVFQIRGLAADKGYDSIFRLSLSRHAKLGTAAVRQRWAYQKLY
                     HLVSVYAAEPRQQVLAEMLKVNQQYGIPIPYESEMK"
     gene            complement(21635..23998)
                     /locus_tag="PLZ74_01400"
     CDS             complement(21635..23998)
                     /locus_tag="PLZ74_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR97008.1"
                     /translation="MRICRAQISAGMLLLAVCGVRAADPVEMRDRLQLADGLFRRSLF
                     DLAAREYVSLAETPQVPDRDNVLFRLAECYRRTGRAAEAAATYKQLSESFPDSPNVAR
                     ADLQRALLLLRAGGAELNVAAALFEKLTATGMPPEVRSAALYHLGETLEKLDKPAEAL
                     ARYEQLSREYAQTDYGMYAGLRIAWLLGKTGKPEDRRRAMGIYLDLAHKARDPKVAEE
                     ALFFAAQTALLDQRHEESANLFKTLKTKYPASPRIRESALSAGWANYYTGRYAEAFES
                     LDLIIGDAGHPAREEVLYLRANCLRQLERRAEAVKVYSQQLEAFPTGRLAGQAGYEKL
                     TTLYHDGAYEAVLKMTSENTRPVDLHADHVYWMNAEAALALQRYDAAVQYCRLLVDKC
                     PQSPLVKDALYRLGWLLQKQEAWESASSWYLQVVQRFPDDPLASRALYASGVCLSRLG
                     QSDAALRDWTALLTRYPECAEVAETLYQKAMEELRAKNVRAAAATLDERMRRFPNDAR
                     KAEVLFWRASAYRQVGEDAEAEKLFRACLAASPTKDFERESMLELGMLLQKTGRKPEA
                     ASFFQKLLDAPIAEKLGAERLAWLAEFQLEQKQPAAAIQAATRLVELKVDKGWTQTGW
                     TLLGRVHRAKGERDPALHAFTEALATGASTAYGAESALRLGELLSEAGRFDDAEKHLT
                     DAASRAATPDLLGLRAHAYAGLARNAERKGDSEAALRYHISVGILFDDPTLVPQSLHQ
                     AAVLLDKSGRAREAAALREELRTRYPASPLANPVPSTAAAGERKAGS"
     gene            complement(23995..24384)
                     /locus_tag="PLZ74_01405"
     CDS             complement(23995..24384)
                     /locus_tag="PLZ74_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOR97009.1"
                     /translation="MTSMMDIIFLMLFFFITTSVFSQWEYEINITLPSAQSGKVPDRL
                     PGEIIINITRDGRVSVNQQDLTLDALRARLDRLARYFPGQPVVLRADKETRYEDLIKV
                     VDTCRKADIWNFSMATSEEKPAATETP"
     gene            complement(24436..25209)
                     /locus_tag="PLZ74_01410"
     CDS             complement(24436..25209)
                     /locus_tag="PLZ74_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOR97010.1"
                     /translation="MVNRKTVTGVLAFTGSLACAWAQEAAESAAAVPALINGYGMSLK
                     QAWEYGGWVMWVLAAISVFALAMVFYFMTVLRSGAITPRELLIDLVARVRANDLTEVR
                     RLCDRHPCPLASVVLAVLDCLRNVPQCDVALLRSTAEAEGARQADAIQGQTEFLLDVA
                     TIAPLLGLLGTVLGMLTAFGSVASDIASAKPVVLAAGVSQAIVTTVFGLIVAIPCMVF
                     YAWFRRRASRQIANLEAASSEVLTALIGMGMKAPFTGQD"
     gene            complement(25265..26656)
                     /gene="argH"
                     /locus_tag="PLZ74_01415"
     CDS             complement(25265..26656)
                     /gene="argH"
                     /locus_tag="PLZ74_01415"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOR97011.1"
                     /translation="MKKETASQKTIVGKIHPDVLAFTVGKDPVLDLDLAVWDCLGTAA
                     HVTMLSRMRVRPAVLGTAERNRVIAALAEVIREIRAGEFRITQADQDVHLAVERRLTE
                     RLGELGRKIHTGRSRNDQVAVDIRLHVRDQLLGLTEEVAEVAGELIRFGCRHAKVPMV
                     GRTHMQPAMPSSVGLWATAYAETLLDDLLMVEAAYTYNDRCPLGSAAGYGVPLPVDRE
                     LTARLLGFSAPHHNVFHASNARGKNEAVLLAACGQLMLSLSRMAEDLILFSMPEFGYF
                     TLPPELCTGSSIMPQKNNPDVLELIRAKAAVVLGYGSAANMILKALPGGYNRDLQETK
                     ALYMEGLTVTRAALRIMRLMVGTLTVRPERLRAGFTPDVFATDRALELVAGGMPFRDA
                     YQHVRSHLETLQEADPDRALAAKTHLGATAGLDFGGYRARVRDVRRWVRVRRTRLRAI
                     FSALLGGAADALG"
     gene            complement(26671..27915)
                     /locus_tag="PLZ74_01420"
     CDS             complement(26671..27915)
                     /locus_tag="PLZ74_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97012.1"
                     /translation="MGHDSRIRRLATAAGWVLSMFAAVGGEPYGPRRTRLAAPAGAIT
                     SRDGSIVVRAPVAMGSYRAPVLVFVDRTRDELARATRLKLVTPSCPLEIVIGNRRDGD
                     TRVLGSRVREPGGGVFERIELPDPEAADLGRFRRAISLALLRAWMVEAGGREGVMRDV
                     PLWLVDGVVRHTGRETRQQDIDRTLLLWSRACLPPAAALFAMESVAASREPAVAAVLA
                     SWLLERRPDGLPFEKLLCAAAEGQEWRPDAVARILTGTEDPLAFDAALDLWFTALGRT
                     VVKPGLTSSGIVRRFRSRLLLYPAFYGTTWGYERPYLTFREAAMRSAEPEVQRRAAVQ
                     ARLIEMAAVGRDGMLLAVSEAYASFLQGVCSGEAQGELLRRLMEAEGMRRELELRTAR
                     GMVLQRADAGRATGAGPRRVRE"
     gene            28020..>28266
                     /locus_tag="PLZ74_01425"
     CDS             28020..>28266
                     /locus_tag="PLZ74_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate epimerase"
                     /protein_id="HOR97013.1"
                     /translation="MLYFECGSKSGALDRTRLKQGLVEALDRLGPRQKVLVLPPDFTR
                     FHSQAGLLTQYVWEYYGDRLAAVMPALGTHSPMTEAQI"
BASE COUNT         5602 a         9572 c         8166 g         4926 t
ORIGIN      
        1 ccgcatgacg cgcgaagtct atgagggccg cgtcttcgcc tatcagatca aaccccacgc
       61 ggaaggcgcg ttccgcgccg gtccggttcg gatcaaggcg ggggataaaa tcatcacaaa
      121 ccccggccct ctcgtgcagg tcaaaggcat cgaggcgcaa gagagcgtgc tggtttcgct
      181 ccgtgcctcc tccacctccg tactggtcga ggagccgctc accctcaccc tcgccgtggc
      241 tgtgaccgaa ctgcccgagc cgtatgccgc ggaaaacgaa ccgctacacc ccaaccggtt
      301 gccgcacctc tccgccgaat tccttgaact ccggcaaaac actgccgggc tcaaaggccc
      361 tgacctgaac cagattctga acggtctgat cgatcagagc ggacagcaac ccgccttcgc
      421 gatcaacgaa tacaccacac agggcatggg gagcggcttc ggccgtctct tcgaccccga
      481 ccccttccgt ccgcgcccga tccggttccg cctcccgcac acgcgcgaga cgatcaacgg
      541 ccggaaatac cgcgtctaca cgctcacgct caactacacc ccgacgcaag agggcgagtt
      601 caccttcgga ccggtcctct tcaaaggtga ggtgattgtg gatgtcagcg ccgaccggaa
      661 ggcgatcacc cgcgacatct acaccatcgg tccggcggtc accgtgcgtg tggtgccgcc
      721 gccggatgag ggacgccccg aaacattcat cggggcggtc ggacgatcca tgcaggtctc
      781 cgcagccttg gacgcgacag tctgtaaggt cggcgacccc ctcacgctca cgctggaaat
      841 ctccggcgcg atcagcgttt ccaacctgcg cacaccgatc ctcaatctgc aaccggaact
      901 cacgcgcgat ttccgtatct acgatgagaa cgtaaaagcc gataccctgc ccaacggcaa
      961 acgcttcaaa taccgcgtgc gtccgacccg cgagggtacg ctggagtttc cgccgatccg
     1021 cctgacctat tatgacacgg aggcgcgcga ataccgtacc ctcaccaccg cgccgatccc
     1081 cctgcaggcg cgtcccacca cacaaatcgc cacaccggat cctgaccgga cggacgtcgg
     1141 tctgaccggc acgctggaga gtcacacacg gccgttgccc gccgggatca cacttgatac
     1201 ccaaggcaca cggcccgatc cgctgttacc acccggacgc gtcatcctgc tgttgatgac
     1261 cgccggaccg gctctctgtc tgctcgcctt ttccgcatcc ccgctcgccg cctgtgccgc
     1321 agcggtccgt acccgccgtc gtcacgcggg tgccctgcgc cgcgccctcg cggctttggg
     1381 ccggacacat acaccggatc gcgcggcgcg gaccgtgcgc cgatatctga ctgaccgtct
     1441 ggacgtggca ggcggcgccc tgacgcccga cgagatcgca gacctgctgc aacagcgcgg
     1501 ggtgccggta aactccgcca ccgcatgccg cgatttgttg gcgcggctgg acgaagcgat
     1561 ttaccggcct gacgccccga tcccggacac aaccctgcac cgggacctga tcgcattgtt
     1621 acccgaaatc gacgccgcac tcgcggagac gtgccgcgtc ccgaccaaag acgaactgtg
     1681 atgagcgctt ttttcaacat cattcttgcg cgcgcggatg ccgtgtccgg catgcgtcgc
     1741 cgcgcattgg tcacgatcag ttgttcgctc ttcgcatgtc acggcgtttt cgccctcccg
     1801 ggagacgaca cggcccgcgt tttcatctgg gaacaggcca acgcgcaaat cgccgttgcg
     1861 acaacgccgg aggcctatca aaaagccgct gaaacctaca accggctggt ggccgacaac
     1921 gtgtgcaacg ggccgctctt cctgaacctc ggcaacgctc tggtgatggc gggagacggc
     1981 atcaacgccg cagccgcctt cagccgcgcc gaacggcatc tcggcaccac ccctgaaacg
     2041 cggcaggggc tggccgccgc gttcgcgctt caaagcggcc gcgcctcgaa cgatctcccg
     2101 tgggaccgga tcgcgtttgt ctggcactat gcgttcccct gccgcatccg cgcatatgcc
     2161 gcattgtgcg gatggtgcct gctctggttc ggcgtgttgt gcctgattcg cgggcggcat
     2221 cgcgaccggc acacctttct gcgcagcctc gccgaaacct gcacgctgac cggcggactt
     2281 ctcgccactg tgtttgcggc ctcggtgctg atcaccctag cgcaggagcg tcatgatgcg
     2341 accacctggg gtgcgcgtgt ctttctggct tcaaccccgg aaacggaggg ctcgccatga
     2401 ccgcaccttg caccggacga cgcatccggc atatgaaaac ctccctccgc accctcgcgg
     2461 caacggttgt gcttctgttt ggcacgggtc ttccgctaca cgccggcgag ttggtctccg
     2521 gccgcctgta caccgttccc gaaacgattt acgtcaatca agcgttcgag atctattttg
     2581 aattggaggt cacgttcggc agcgaggtgg aagacctccg catccgtgac ttcccgaacc
     2641 agcccgacct cctcacggtc gggaggttgg tgagttcctc ccgtaaccgg acttcgcgcg
     2701 acggcaagac tctggacacc ctgcagtata aagccaccgc ccgctgtcac aaaccgttcc
     2761 accaaacttt cacgccgctg gtgcagtgca tgctggtcga acgccggaac accggcttct
     2821 tctcgcattg gcagtccttc cccaagcaga tgcggcttga ccccttcacc ctcgcggtgc
     2881 gcgatctgcc tgatcaggga cgaccggaag cctttagcgg cgcgatcggg acattccgtc
     2941 tgaccgggcg tctctcccaa accgaggccc atcccggcga catcatcacg ttgacgctga
     3001 acctcaaagg caaaggttgg ctcggcgaac agaccgttat gccggcaccg gccgttggtc
     3061 cgatgttcaa gcggtatccg gtcaaagaac tcctgcgcga ggatgcgcat ctccgtaccg
     3121 agcagatttt cattccgcaa accaccaacg ccaccgagat cgccgccgtg cgcttccatt
     3181 atttcaaccc cgcgaccgga cagtacgaag cgagcgttgc cgggccgttc cggctgacat
     3241 tgcgcgacgc acctgcggaa ccggtcgcgc agacgatccg gatgatcgac accgtacgcc
     3301 cgacaaccgc atcggaaccg ccgcagacca tttcgatccg tcaggtcaac cgttcggtgc
     3361 ggcacgcatt gccgttgctg atcggcggcg cgggtgcgct ggccgctttc ttcatcatgt
     3421 tcctgctggg tgccgggcgc aggcggatcg ctattccgct cgcgctgttg gcactgggag
     3481 tgagtctggg cgccgcatac cggatccaca tccgcgcaga cgccgccaca cgtaacctga
     3541 ccgagcaggt tgacgtgcgt ctcgccccgg cgcgagccgc cttccaactc ttctccctgc
     3601 ctgcgggcac ggaggtcatt ccgttggaaa cttcggcgtc gtgggtgcgc atcgatgcgg
     3661 acggacggcg cgggtggatc cgcgccgagg cgttgggcga tccgacaacc gttccgaaag
     3721 gcgcgccatg aaagaacggc tcgacaccct gatggtccgt gcgggactgg cggagagcaa
     3781 agagcgggcg caacgtctga ttctggcagg cgaggtgttt gccgccggcc agcttgccac
     3841 caaggccgga cacaagtatc cggttgatat cgagttaacc gtcaaagcgc agccgcgttt
     3901 cgtcagccgg ggtggcgaga agctcgaagg cgcgttttgc gcattccccg agttcaacgt
     3961 caccggtcgg gtctgcctcg acgtgggggc ctccaccggc gggttcaccg actgcctgtt
     4021 gcagcacggc gcggcgcgcg taatcgcgct ggatgtcggg aaaggacagt tgcactggtc
     4081 cctgcgtcag gatccgcggg tgcaggtcat ggaatccttc aacgcccggt atctgcgttt
     4141 gagcgatctg cccgagcaac cgcaaatcgg ggtgacggac gtctccttca tctccctgag
     4201 acacatcctg ccgccgatga gcgacgtcct gccttcgggc ggcgagatca tttcgctcat
     4261 caaaccgcaa ttcgaggcgg ggcgcgagaa cgttccgggc ggcgtggtac gagaccccgc
     4321 agtacgggaa gccgtggtgg aatccattcg agccttcggc acggagttgc tccacctgaa
     4381 ctggctcggc tgcgtcgcct cgcccctgcg cggtcccgaa ggtaacgtcg aatacctcgc
     4441 ctggtggcgc aagccgtagc ttgccgaagg cggtcacaga gtccgggcgc agcaggcacg
     4501 ggtgcgtaaa aagcgcaacg gccatgccga tcacgatccg cggaaagaaa ggtccggcgc
     4561 ctcgctgacc ggcacagccg cgccgggctt cacttcttcc gcaacatgga aagagacacc
     4621 cccacaaaaa ggaggacggc aaccgtcagt ccggcggttc ccaacgtgct tcgcagtgta
     4681 cctccgccgt ccggctgcaa ggcaaggatc aaggccgacc ccagaaaaag agcgccgccc
     4741 ataatgctga gcgccagttg cgccgtcatg cggcttacct gtcgaacatg tttttccaac
     4801 ccctcatgtc gataggcaac cacctgcttg ccctcgctca ggttcgtcat gatcgtgccg
     4861 attgccgtgg gcagggcttt gatcgtatcc gcgctgtcca tcgcgaattg actgatcccg
     4921 ccgctcagat tggaaaaggt aacacgggac cggatcgcct cgcggacata cggcgcgagc
     4981 gctttgacaa agtttatgcg cggcgacagc atcagcacaa tgctttccgt catgacggtg
     5041 gtccggcaaa gcatgatcaa ctgctgcgga atgatcagct tgtgcgtggc gaggagattt
     5101 atgcccgttt ccagcaggac gtcgagacgc agatcctcca tggggagatc atagtagaac
     5161 acgatcagtt cgtgcagttc ccgggccaac tgcttgacat cgacctgaga gccgggttcg
     5221 gcataggccg caagaagctt gctgagccga acggcatcct tcgtcatcac ggcgacgaac
     5281 atgtccgtca tcagttgccg ggtttccgga tcaatcgtcc gcaccatccc gaaatcgaac
     5341 aaacccaaaa catggtccgg taaaacgatg atgttgcccg catgcacatc ggcgttgaat
     5401 aaaccatgcc ggaagatttg cttggccatt aaggagacca gatgcttcga cagtgtgtca
     5461 cggtcaaccc cctgtcggac taactcgtcg agttgatcga tcttgacccc atcgagcttt
     5521 tccagcacaa gcagtttgtc ggtgcacagg tccttataca tgaccgggat ccgaacggtc
     5581 gggtctcccg cgaaaatggt tttgaacttc aaaccattat gcatttcgcg gtggaaatcc
     5641 atttccatca gaatcgtctt atggaactcg ttgaccatgc tgcgcagatt caagcgccgg
     5701 aactcctcca catatcgctc aagcagaggc agaaaccggg ttagaatctc gatgtcggtt
     5761 gcgacaatgg tctggattcc ggggcgctgt atcttgacga caacggattc gccggttgac
     5821 gccaagcggg cctcatgtac ctgcgagagg gaggcggccc cgatcggcac ggtcgaaaac
     5881 tccgaaaaca catcccgcag cgggcacccg tattcggtct cgacgacaca gatagcgtcc
     5941 tgcgcgggaa tggcggcgac attgtcttgc agacgggcaa gctcaagggc gataccgtct
     6001 gggagaatat cgcgccgggt gctcaggatt tgacccaact tgacgaaggt cggccccagc
     6061 gactcgagcg ctttgcgcag attttccccg atacgggcgt tcaagtcagc gggtgcgcca
     6121 cccgccctga cggaccggcg agcctggatt ttggccttca ggcaagccca tccgcatcgc
     6181 gcgaacacga caaaaatctg ccgcatgcgc ttcgcgttcc gatagggacg gatcatcatc
     6241 agccgagtca aacgcataca aatccccatc gcgcgcgagg gtgatgcgcc cccgaagggg
     6301 caccgctagc gcagcgattc tgcctgacat cgctgaacgg gccgcccgcc gccatgccgc
     6361 cagcgaaatt cataacgcga tcaggccttt gttgcggcgc ggggtgcttt catcgccgcc
     6421 ttcaacccgc tgatctcttt gtgaattttg gcgagatcgt ccgccgtcac gagtcctaac
     6481 tcgcccaaga ctttcttcgc cgaatcccga aattgatctc cgactttctt tttgcatgcg
     6541 gcaccctcgg attgcgcctc acgcagaaaa gcctgcgact gctgtttgct caactcgcct
     6601 tttttcacga gcgattcgac tgccgcaacc agtttcttct ggcccgtcac agccaagccc
     6661 agcacaatct cgactgcaca cacgccctct tttttcatat caaacactcc tctgttactg
     6721 cctatccgtc tcggtatgga aaatccggaa aactccggtc ccaacaatga gattatggag
     6781 ggaacggatg aaaagagtca accccgaaaa gtgcgcaaaa atccaacgct ccaacgcggc
     6841 gggcacattc gtttgctgga acttgaagcg cctcaccgtt tctggcaaaa tacgccacca
     6901 aatggtccga gcaggttccg ccatcacgat tgaagccccg gccaagatca acctgaccct
     6961 ggagatcctc ggcaagcggc ctgacgggta tcacgagttg cgcagcatcg tgatgccggt
     7021 cagcctgtcg gatacgttgc gcctgaccac cacctacgcg cagatcgcca tgaccatgac
     7081 ggtcagtccc ggcgtggacc tcacgaagat cggcaccgtg gaccagaacc tcgtggtgcg
     7141 caccgcccgg ctgatgcaat cacgctaccg ggtcaatgcc ggcgcgcgca tccacatcca
     7201 caaacgcatc ccgatcggcg gcggtctggg agggggcagc gccgacgccg caggcgtgat
     7261 ccgcgggctg gaccggctct ggtgcctaaa ccggccgcgc gaggaactca tggcgttggc
     7321 cggcgagttg ggtagcgacg tgccggccct gttccatggc ggagtcgtgc tcatggaggg
     7381 gcggggcgaa cgggtgcggg ccctttttga cagtaccgac ccaatatgcg gcgacggttt
     7441 ctggctggtg atcgccaacc ccggagtcat gtgccccacc tcagaggttt acaagaagtg
     7501 gagagggggc ttgacggcag ggccttcgat tcttcataat atgatttctt cgatccggac
     7561 cagcaatgtg gaaactgcgg cggatgcgct ttataacgga ttgcaggccg gggtatttga
     7621 aggttacccc ggcgtcgctc agacagccgt gaaactgcgt gaggcaggtt gtctgggtgt
     7681 tttgctttcc ggtagcggcg cgtcggtctt cgggttagtc cgggacgaag cacatgggcg
     7741 ggagatcagc cagaggctgg ataacgacgt gtggcacgta ttggcgcgca ccttgcccgg
     7801 tggtgtaatg gcagcacaag agactttgac tctctcagag gtggttcgaa tccaccccgg
     7861 gcaaccagtt tgtgtttggg aaagtgtggg cgagcatgat tgttttttca ggaaccgcaa
     7921 acctaccgct ggccgagcgg atgatggcat acctcggcag ccggctgggc gacatcaaca
     7981 tccgtcgttt tcctgacggc gaaattcacg tcaagattgt cgacaatgta cgcggcaggg
     8041 acgtcttcat cgttcaaccg atctgcacgc agcccaacga gatgctgatg gagctgttga
     8101 tcatgatcga tgccgcgcgc cggggttctg cggcgcgtat caccgctgtg ctcccctact
     8161 acggctatgc gcgccaagac cgcaaagacc agccgcgcgt gccgatcacc gcgaaactcg
     8221 tggccaacct gctggtggcc gctggtgcca atcgcgtgct gaccatggac ctgcacgccc
     8281 agcaaatcca aggatttttt gatattcccg tcgaccacct gcacgccgcg ccggtaatgg
     8341 tgaaatacct gcgcgaaatc gagctcaaaa agccgctggt cgtgtcgccc gataccggag
     8401 gggcaaaagc ggcttacgct tattcacaaa tgctgggcac cggcctggcg attgtggcga
     8461 agcagaggct gggagacgcg gaagtggacg cctttagcgt cgtgggtgac gtgaaagggt
     8521 gtgacgtgat catggttgat gacatgacca cgacctgcgg cacgctgtgt gcggcagcga
     8581 aaatcctgaa ggagcagggg gccgcgtcca tccgggcggc ggtgacccac atgcccctga
     8641 cggataaagg tgtacagcgg cttcaagccg acaccgggct gacggaactg gtggtcacgg
     8701 acacggtccc cctgcgggaa ggtctggact ccgccgccat accggtcaag ctcacggtgc
     8761 tgtctgtcgc cgacctgctg ggcgaagcga ttcgcaggat ccacaatgac cagtccgtca
     8821 gctcgctttt caggtattga cgggcttttt tcacactgac agaggaaatc atggcgaacg
     8881 aaaagatcag aatgaaagcc gagttgcgca cggagcaggg ttccggcgcg gccggccgtt
     8941 tacgccgtgc cgggagcgtt cccggtgcgg tgaaccggat cgagggcggc acgacgctgg
     9001 tcaagattga tgcgcatgcg tttaaaacca tgctgcgtca tcatggccgg gagcagatgc
     9061 tggtcacact cgagatggac gacaaggaag tgtccgcgct gatgcgcgag gtgcagtacg
     9121 atgcgatcac cgggtatccg atccatgtgg atttcggtga agtctccctg acggagaaga
     9181 tccgcgtggc gctcccgctg caactgacgg gcgagcccga gggcgtcaag atgggcggcg
     9241 gcgttctgca gcagatgctc cgcacgatcg acgtcgagtg cctgcctacc gatttggtcg
     9301 agcatttcga ggtggatgtc tccggcataa aactggggca gtcgcttttc gtgcgcgacc
     9361 tgaaactcgg cgacgcctac acgcttttca ccagtaggga cggtgtggtg gccacagttg
     9421 ccgcgccgga ggaagaggag gaggcgacgg ccgcgacgcc ggaagctgct gcggccgcga
     9481 cgccggaagt gatcaccaag ggcaagaagg aggaggatgg cgaggcctcc gctccggcgg
     9541 ccaagaagta atccggagga cacgaccgcg tgaaaatgat tgcgggcttg ggtaaccccg
     9601 gcgagatgta cgcgaacacg ccgcacaatg tcggtttcga cgttgtcgac ctgttggctg
     9661 aacggctcgg ttgcggatgg aaacgcagtg cccggtttca cgccttcgtc tcgcggaccc
     9721 gttgcgcgga ggagccgatc ctgctggtca aacctcagac ttttatgaat ctgagtggaa
     9781 ccagcgtggt tccgttgctt cgctactacg ggggtgagcc ggatgacctg attgtggtgc
     9841 tggatgacgc tgacttgccg ttgggcatgt tgcgcatccg ggcggcagga ggcaccggag
     9901 gacaccgcgg actgacttcc gtgattgagg cgctgggaag cgacgccttc acgcgggtcc
     9961 gtctgggtgt gggacgcgaa ggacgcggaa gtctggccga tcatgtgctg ggcaagtttg
    10021 acgaagcccg ccaagagaag gcccgggcgg ttgtcgaagc cgccgccgac gcggtgcagt
    10081 gtctgaccga aaagggattg aacgaagcga tgaaccggta caacggctgg cgggcggatg
    10141 cgccgctgcc gccggacgac cggaagtgag atgggaaacc aatgatgaag aaatacgaag
    10201 gcttgtatat cttcgccggc tcggcgaaag atgacgtgct ggataagcag gtcgaaaaag
    10261 tgctcggaga gatcacgcgc ctgtctggca acgtgatcag tacggatgtg ctgggcaaac
    10321 gcacctttgc ccgcccgatg aacaaacgcg aaaacggcgt gtacgtgaag atccgttttg
    10381 agctggaccc ccagcacgtc acggatctga aaaaccgcta ccggttgctt gaagacctct
    10441 tccgcgtgca gattctggtg gtcgacgaac gccgcgagag cgtgctcgcc cagcaggagg
    10501 aggcgcacaa ggcccgtgcc gcagctcgcg cagcggcagc ggaagcggag gcagaagcgg
    10561 cagcggaagc agaagcggca gcggaagcgg cagcggaagc tgcagaggaa gaggaagaag
    10621 aggtggaatc tcaagacgag cgcgatgcgt aaacgcgacg cgcggcggaa aggacggtga
    10681 gacatggcat ccttcaacaa agtcatcctg atgggcaacc tcacgcggga ccccgaactg
    10741 cggcagactc cgaccggcgc gaaagtcgcc gaactgcgtc tagccgtttc agagacctgg
    10801 cgcgacaaaa gcggacagtc caaagaggtg acctgttttg tggatgtcgt ggtctgggaa
    10861 aaactggcgg aactctgcca ccggtacctc tccaaaggca ggccggtgct cgtcgaaggg
    10921 cgcttgcagt tggacgagtg gaaaaatcag caaggggaaa cccgaagcaa attgcgtgtg
    10981 cgcgccgata cggttaaatt cgtcggccca ccgcccgccc gttccggaga gacgccaacc
    11041 gcggcacagc cagcagcccc tgacgcgtcc gtacccgttg cggaccgtac ggattacaac
    11101 gtcccggacg acctcaccga agatgatgag cacatcccgt tctaaaccac acgagcgatt
    11161 aaccaaagga tcatgtctat ggccatgcaa agaaaatcaa cttctcaccg cggaaaccgc
    11221 gaaagccgtg accgcgacag cggcccggcg cgcaagcgcg cgcggcttct ggacccgaac
    11281 gacatcatcg atgtgaacga tgtcgaattc ctgcgccggt tcaccaccga atacggcaaa
    11341 atcctgcctg cacgcctgac cggcgtgaca gctgcacagc aacgccagat caaactgggc
    11401 gtgcggcgcg cgcgcaacat gggcttactg gcctagcccg gaaaggaacg ttacattatg
    11461 tcaactgaac tgttactgct ttccgacatc cctgacctcg gtcaggccgg tgatgtcgtt
    11521 aaagtggcgc ccgggtacgc gcgcaattat ctgttgccga aagatctggc cgccccggtt
    11581 tccccccagg ctctgcgccg cttggaaaag ttgcgtaagg agcgtgagga acagacacgc
    11641 ctccgactgg ccgaggcgaa cgccaaggcc gccaaactga cgaacgcctc cgtcaccctc
    11701 cgcgccaaaa ccgtggacgg cacgcgtctg tacggctcga tttctgccgc cgacatcgcg
    11761 gccgccatcg agacggagaa caaggtgact gtggacaagt cgcagataga actgcccgag
    11821 ccgatcaaag agaccggcac ctttgacatt tccatcgtcc tgcatgacga tgtgaagcct
    11881 gtcatcaagg tctgggtagt cgaagactga ggcctccgca acacacaaaa aggagtcagg
    11941 cgtgaggcat cagggatacc caccggttcc gtgagcctca cgtctgcttt tttcgatcat
    12001 gtcccccacc ccgcccaccc caaagccgtc ctcacgtatc ccgccccata gcatggaggc
    12061 ggagcgcggt gtgctcggct ccattctgct cgatgcggcc acggacaacc gtgtgcttga
    12121 tctgtgcatc gagagcggga tcacggcgga ttcgtttttc tccctcccca accggctttt
    12181 gttcgaaacg ctgtccgaga tggcccgcgc cagccagatc atcgatccgc tcaccctcac
    12241 ggaacgcttg cgcgcactta accggctgga agagatcggc ggcgccgttg ccatccagac
    12301 gctgatcgac gaaactccca ccgccgcgca tgccgagtat tacatcaaca tcctgcgaca
    12361 gaaacatctg ttgcgcgcgg tcatcacctg tgcccgcgat gccgaacgcc gctgtttcga
    12421 cgaggccatc agcgcggacc tgatcctgag cgaagtcgag cagaagttcc tctccattgc
    12481 cgaccgtcgc gatggggccg gactcacctg gccgcaggcc atcaccaata cgatggggca
    12541 tatcgaccgg cttttctcgc tcggtcccgg tgccgtggcc gggctcccca ccggcctcgc
    12601 caacctcgac cgtaaactca aagggctgag gaatggcgag atgatcgtgc tcgccgcgcg
    12661 cccctccatg ggcaaaacct cactggccat gaacattgcc gagcgtgtcg ctttgggcaa
    12721 agacatgaac gggcgcgcca tgaagggcga ccacaaccgc ccccatcccg tcgcggtgtt
    12781 ctcgcttgag atgtccaccg aatcccttgc catgcgtatg ctctgtggcc tcgcggaagt
    12841 gccgagcttc aaaatcgacc aggggttggt aaacccaaaa aagattaacc ggcaactgac
    12901 tgatgcggca tccaaactcg ctaaagcgcc gatttatgtt gacgacaccg gcggtcttga
    12961 cgtcatggac atgcgcgccc gcgcccgtcg catgaagaag cgttacaagg tcgagttgat
    13021 tgtgatcgac tacctccagc tctgcaactg ccgcgaattc gcccggcagg gccgccagat
    13081 tgaaacctca cagatctccg gccagatcaa agcaatggcc aaagagttaa acctgccggt
    13141 catcgtgctc agtcagctca gccgcgcccc ggaacagcgc ggcgataaaa caaacaagcc
    13201 caaactctcc gacctgcgtg actccggtgc catcgaacag gatgccgacg tcgtcctgct
    13261 cctgcgtcgt ccgtgtaaag catccggaga tccggagttt gatgacaagc tgctcgccat
    13321 tgtagacgtg gccaaacacc gtaacggccc gacgggcgag gtgcgcctga acttcgagga
    13381 caccctaacc cgtttcagcg accgcaccga ccagatcacg gaggatgacc ggttcgaacc
    13441 cgacccggac tcctgacgga cccgcctgat tctccgccgc agcggttgcg caaggacggg
    13501 cgcgcgcgaa cagatccgca tcctcttccc gaagatacgc cagcgcatcc gcttcagcgc
    13561 ggcgcggcgc gtctgcggca caccatctcg cgcgcctgct ccagcgtggc aagctgtccc
    13621 tcaagctgcg catcgtacag ttcccgcagg agcttcccca cctgcggtcc ggacggcacc
    13681 ccccacgcga tcagatcccg gccgcgcacc agcggttcgg gcagtacagg ttcggaaacg
    13741 aaagcggcgt aggcctccac aagttgccgc caaggagtga gatcgccgtt gctgtgctgt
    13801 atgtccagcc gcatcaactc caacatccgc ggaaacgtgg gctctcccat gaagcgccgc
    13861 aaggtcgcgc gccgcatctt gccggactcg accccccgca tgtgccccgc caccaacgcg
    13921 cgaacctcgg cgatccgcgc agacggctga cgcaaccggg tgagaagttc ttcagccatt
    13981 gccgcgccga ccggcgcatg gcaggggaaa cggatgcgga ccgcaccggt tgcaggatcg
    14041 gtttcctcac ggcacgtcat cggtttgccg agatcatgga ggagcaccga ccaagccaag
    14101 gtcagatcgc gcggcggcgg cagggcatcc aacatcagac aggtatgggt ccagacatcc
    14161 ccctcgggat gaaatcgggg aggctgagcc gttccgtaca ggcgggccac ttcgggcagg
    14221 atcggtgaca gcagacccgt ctcccgcatc aaattcagag ccgttgaggg caagggcgcc
    14281 tcgcacaata tgcggttcaa ctcatgggcc acacgctcgg gactgacctc cgtgatgcgg
    14341 ggcgcccaga cctccagggc ctcgcgcgta cgcggctcca ccgtgaactc gagtacggcc
    14401 gcgaatcgca ccgcacgcaa catacgcagg cggtcctcac ggaatcgcgc cgcaggatca
    14461 ccgatcgccc ggatcacctg cgcctgcaaa tcagtcaacc cgcccacata atcggtcacg
    14521 tcgcccgtag cgggatcgca gaacagaccg ttcactgtaa agtcacggcg caacgcatcg
    14581 ccttccgcgt tggtgaacgc gatctgctca ggatgccgac catcgcggta cggaccatcg
    14641 gtccggaagg tggccacatc aaaggagaag ccgttgcgcc gcgccaccac cacaccgaac
    14701 gccttgccga ccgccaccgt ctccccggga aacaacgcct ccacttgatc cggtgtcgcg
    14761 gcggtcgcca gatcgatatc ctgaactggc cgacccaaca gggcatcccg cacgcatccg
    14821 cctgcgaaaa aagtctcgta tccggcgcgg ttcagatcca aggccagcgc ccgcgcggcc
    14881 tccgcgcgcg ggatatcccc ggcccggaac cgcacctgca caccgttgcc gcccatcact
    14941 caccccccgc ccggatacgg aaaaagaggg tgtcggccaa gccgggcatc gccatcccgg
    15001 cgcggaaacg atgaaccccg ttggtctcgc catcgcgaac cggcgcgaca gacatccctc
    15061 cccacccgtc tgtccgcagc aatgcgttgg tctgcacctg atacggaatc ccccgctccc
    15121 caagaaaatc aatgcagaac gcatcgttcg tcacggcaaa caggagaatg tccggcgtca
    15181 gccaaggttg cggcacatca ccagtccaga tcacatcatc cacccacccc gcatcgctgc
    15241 tgctggcttc agcaccgttc ttacgatact cccaccgcaa cgtatgccag gttccggtac
    15301 cgctcaccgt attcgtcacc gcagtccacg gggacttggt gccggaaatc tccatgaccg
    15361 gagtcccgtc cacgagaaac cgtagaaaat cggcaccggg ctcactcgac acacgccacc
    15421 agaagctcac cgtgccgctg ccccaaacct gaagttcgaa agccgtgaag ccgttatcgg
    15481 gaaccacccc cggaccccca agtttaaccg ccggcgtacc ggagggcgcg ccggacatcg
    15541 cgtcaccggt aagaccgtca accactcctt cacacgtgaa tgcgggcccg taacacccga
    15601 gcagacgcgc cacggaatcg cgatacgagg caatcagcgt caccgcctgc gacggcatgg
    15661 tgagcaccgt gctcggtgcc gtcgcattcg ccagtgccga agtcgcgtca ccattccatc
    15721 cggcgaactc ctgataaagg ggcgcgggat ctgccgccac catcacggcc gagccctctg
    15781 cataccaccc gcttccagag ccgcaaacaa ccgtgagggg cacccgatag gtggccgcca
    15841 cacacaatgc ggaatccgtg gtcgttacag tggtggtcgg cgcgaaccga tccgccaccg
    15901 ttgccacgtc accggtccag caatcaaact gttttccttc cggcacctcc gcgccgatca
    15961 ccacctgcga gccgtaggga tacacaccgg aaccactgcc gtggatcacc gtgagcggat
    16021 aacccgtagc ctggtacgac gcggccaaaa ccatccccgt tccgctcatt gtcacggtcg
    16081 tcgatgctgc cagcacgtcc gccacactgt tggtgtcacc ggtccaccgg tcgaacacat
    16141 gatacgcggg cggcgaatcg gcctgaatcg ccacaaccgt gccgttgctg tacgcgcccc
    16201 cgccgctgcc gtggatcacc gtgagcggat ggaacgggac ccatcgcaag gcgtcaaccc
    16261 agccgcagtc ctgcccgaca taatcacctt gcggatcctt ttcatactcc caccggaaga
    16321 ggtgcggcgt ctcttgaccg ccctccacca gaatcgacat ctcctcccag ttgttcatga
    16381 tgcctgagat ttgatggatc gaccccgtgc cggcctcgta gaaacgcaac acatcccagt
    16441 cagcctcgct cgaagtgcgc cagcagaacg agagcaggcc cggaccggtc accgaccact
    16501 cgatgcccgc catctggccg tccgcaatga tccctttccc gcccagacag acagaagttc
    16561 cgccgtcacc gcagtggttg gtggaaactt gaacggcatt cgtaaacccg atcggcacgg
    16621 catcggacgc gccaccaaca acgatcagcg catccgcgcg ccagtgcgcg tacacggtat
    16681 ggttggtaac cgtacgcatc accgtatctt cggtcactgg cgttccggca ccgccgggtc
    16741 cggaccgcca tccctcccac acgtaacccg tccgttccgg cacgggcaaa tccccgtaaa
    16801 ccccgccaag atccaccaca gccgtggcgg gctcgaccgt cccgcccaag gcgtcgaacg
    16861 tgaccgtgca ggcccgggcg aacccccgca accgcggata cccctcccct tccacgatct
    16921 gccagtcccc gatgaaatcc cagcccgaat aggtcacggc ctgacgcatg gccgatgtgg
    16981 tttgcggcac gcccccggct gaaccgctct gtccgctgac ctcggtatcc caaaacgaac
    17041 ctgcggtgat cccatccccc cagccgatca gcccgcccac accactggat gtgcccgtga
    17101 ccacgcaagc cgcatacgtc tgggtgactg agcccccgtc ggcatgcagt ccgatcagcc
    17161 cgcccacatt cgactggccc gccacactcc ccacggcata cgcgcggttc accgtaccct
    17221 cgcgattctc gccgatgagg ccgccgatcc cgttgtacgt gccgttcacc tgcgcctccg
    17281 cgaaacaccg ctccaccgta ccgcccgcat tgtatcccac gagcgcgcca agccccccgt
    17341 tcccgcacac ctcggcatag gtcaacccga gattccgaat caccgcaccc ggtccgagaa
    17401 cgccgaacaa ccccgcataa tcctcctcgg gacgatccac cttcagccgg gcgatcacat
    17461 tggaccgacc gtcgaaactg ccggtgaacg gcgttgtcat gctgccgatc ggaccgaacc
    17521 cgtccggcca tcccgccgtc tctccggcat acagcatccc ggtcagccaa tagcggccat
    17581 tcagcgtata gtccggatcg cggccgatcc gctgcagttc ctgaaccgat ccgatacccc
    17641 gcgccgaacg gaagatcgcg gtcaggctga catgcccggc catggtcacc tgcgtcaccg
    17701 gattcgtcgg ctgggccacg ccgcccgacc agcggacgaa ttcgctgacg cccgccaccg
    17761 gctgcgcggt caccgtcaca ttcgtcccgg gcgtgtaaga ggagagcaac ggggtgacga
    17821 atacgttgcc cgggccgcgc gacgcaaccg tcaacacaaa ctcgttcccg ttgaccgcca
    17881 gcccgcgcag ataggggtag ctcacactct ccacgatacc ccacacctga ctgaaatccc
    17941 atccggaaaa ggtcacggcc tgacgcatct ccgccgaagt tttgcctgtc cccccgcccc
    18001 atgaggagga ttggccgctt gcctgcgtgt cccaataggc tccggagaca acactgttga
    18061 acccgctccc gctgcccaca agaccgcccg tgtcttccgt gccgctcacc cgtgcgatcg
    18121 cataactctc tccaatcgtc cccccctcat acaggtaccc gacgagtccg ccagccccca
    18181 cgtcggccac aacgcttccc gacgcggacg aacgctcgat cccgcccaaa tccacaaagc
    18241 cggccactcc gcccacggtt tccaaaccga ccacatcccc agaaaccgaa cactcccgga
    18301 tcaggccctc agacagatac ccggccacac caccgacatc aaccagcccc gcgaccgtga
    18361 tccccgtcag acagagattg ctgaccaccc cgcccgtccc aatccggccg aacaggccga
    18421 catcactctc gccgggacgg ttgagggtca acccggtgat cctatggccg ttgccgttga
    18481 acacaccggt gaacccccac tccgcatccc ggccgatcgg cacaaacccc gcgcccccgt
    18541 tccaatccgc cgtggccgag gcgtccagat tcgtcatcaa cgtatagaca gcatcgggct
    18601 cccacccgtc gataccggac cccaccttct gaaggtcatc cagagaccag acctccagac
    18661 ccgccgcaga gagcgcacct gccaggcata ggcaacccgc aaggcggcgc actgtgcgcg
    18721 ccgcattcgc ttgccctgcc gtgtatcgcg gtggctgatt actgagtcct gtttttttca
    18781 tgcggccttt tttcccacaa cccgatccgt tagctcagga ttgtacccgt acacacgtcg
    18841 aatttcaacc tctgacacaa gcaggccatc ttcccgattc ccctttcggc tttgacaaac
    18901 gaaacgtcga aggggtacac tgtcgcacca tgcctcatcg aactttgagt ttgcagcaag
    18961 ccgcgcgcca catccggatt ccggaacgcg agctgttcca tttggttcag cgcggagaga
    19021 tcccgtttct gagacacggc gacgaagtgg tcttcgagca ccgtcgactc gacgattggg
    19081 cgcaacggcg catcctcggc ctgccgacaa aatccctgac ccagcatcac cgcgaaacaa
    19141 cggcggaacg cacgccccat agcaaagacg atgccttggt ggcgcgactc ttccgtcccg
    19201 aatggatcga ccccgccctg ccctcgcgga ccaagcccgc agtcgtgcgg gacatggtcg
    19261 cgttggcggt ctcgaccgga ctgctgtatg acgacgcggc ctttcaacgc gaggtcgaag
    19321 agcgggaggc cgcctgttcg accgccatcg ggggcggcgc ggccctgttg catgcgcggt
    19381 atcacgaccc cttctgggct tcggattcgt ttatcgtgct cggcaagacg cagcagaaca
    19441 tttttttcgg cgcgcaggac ggcggcgaga cggatttgtt tttcctgatc tgctgtacgg
    19501 atgacgcctt gcatctgcat gcactggcac ggttatgtat gctcgcacac ggcagcctgc
    19561 tcctctccga cctgcgcgcc accgaccgag ccgacgagat gcatcaaatc ctgctgcgcg
    19621 ccgaaagcga actcctgcgc accctgtgag ccgggtcatt tcatctcgga ctcatacgga
    19681 atcgggatac cgtactgctg gttcactttg agcatttcgg ccagcacctg ctgccgcggt
    19741 tcggccgcat agacgctgac cagatggtac agcttctgat acgcccaacg ctgccttacc
    19801 gccgccgtgc cgagtttggc gtgtcgcgac aggctgagcc ggaaaatcga atcgtacccc
    19861 ttgtcagcag ccaggccgcg gatctgaaac accagttcct ccgtggtgtc gggacagacc
    19921 ccgtgcaact ggatctgacg atccttgtag agaggcgtcg tgcgtttcgg gtagacctcg
    19981 ctctgcgaca cactgtcgaa cgtcacctgg atatcgctca tcaccggata acgcagccca
    20041 gtgtacgctc cggccatcga gtccggaata tcccaccgat taccggtgag cacaaccgac
    20101 ccgccccgat tcccgtacgt caacatatcg agcagataga cattggcttt gctctgtgtc
    20161 ccgtacatgt tgacggagac caccccctga ttcagcttcg agaactcgcc aatgatcttg
    20221 gcgctctcgg tcatgccaaa ggtcggcttg ccgtccgtca caatgaaggc gatcatcggg
    20281 cgtttcggat cacgcggcag tttcatgagt gtgcgcaacg aaccgaacac gtccgtctgg
    20341 ccgaacgcgc gcatggccgc gacaaaagtt gaggctttct tgaggtgggc ctgggtcacc
    20401 ggaacccagt ccggaaaaca catttcggtg gtgtcccgga acgcgaccac gttaaagcgg
    20461 tctccggggt tcatcgtttc cagcgcggcc gtcatcgcgc ggcggcagaa gaccatgcgc
    20521 tcctcggaca tcgaggctga gacatcctga acccagacga tatccttgtc gagcgtgggc
    20581 accgtgcggt cgcggcgcgg ctcgattccg atccggaaat agatgcgtcc gggatccgcc
    20641 gggtcccggt aggtttccaa gcccacagcc aacagatcgt cgatcggctg atagtcggcc
    20701 agctcctgca acgccgcgat ctgggcctgg gcctcacgcg ccttgcgctc ttcctcgctg
    20761 acttcagtag cggcgatctg ttgcgatacg gcggccagtt cacgttcgat ccgtttctgc
    20821 tgctccgagg tgctcgcccc gtccggtcca gccgttgccg agccggtgtc cgctgccgag
    20881 ccggtggtcg ggcttttccc tgctttttcg ccgggctgca tcgcaaaccg ggactccgtg
    20941 gctgcggtgg agaccggcgc gggcgtggcc ggcgcctccg gcgcgggaac cttgaaggta
    21001 cccgcgctga tttcggtatc aaacacctcc gccgcctcga atggcttggg cggctcaggg
    21061 tcgttgccaa aacggcgtcc cacgaggtcg atcgacggca cgatgtccgg cgcgtcggga
    21121 acccgttcaa tcatggggat ctcgcggcgc ggcagagtcg cgacctcatc caccacggtc
    21181 cggtcataaa tctgggcgat ctcctgacgg ggcatccagg gggtggtgtc gatctggggc
    21241 gaggccgcct ccttgagcgc gggcacaccc ggttgaagcg cttcccgcgg aatcggcggg
    21301 ggcgcggtga gaacgggcgc ggccgactga ctgagcgcct ccacctgctc ggaaacctcg
    21361 atcgggccgc ggctgggcgt atcgcgctca cccggcttct tccgaccgat atacatgggg
    21421 tccgcgcgca tggtttcgac acgcatgggc ggcacgcggt cgcccgtgaa ccattcgcgg
    21481 ccccgttcgc gcaaactgga gatgccggaa atcgtccaat cggagaaata gaccatcaag
    21541 accacatgga tcaccgcgga caacaggacc gcgacacagg ccagcagcag atgacggtca
    21601 tgggattgcg gatgcggtct gaatccgtat ggtgtcatga gcctgccttc ctctcgcctg
    21661 cggccgcggt cgacggaacc ggattcgcca acggcgaggc gggatagcgg gtgcgcaact
    21721 cctcacgcaa cgccgcagcc tcccgggccc gcccggactt atccagcaag accgccgcct
    21781 gatgcaacga ctgtggcacg agtgtgggat catcgaacaa aatcccgaca ctgatatgat
    21841 agcgcaacgc ggcctctgaa tctcccttgc gctcggcgtt gcgcgccaat ccggcatagg
    21901 catgcgcccg cagccctaac aggtcgggcg tggccgcgcg cgacgccgcg tcggtcagat
    21961 gtttctccgc gtcgtcgaac cgccccgctt cgctcaacaa ctcgccaagc cggagagcgg
    22021 actccgcgcc gtatgcggta gaagcgcccg tggccagcgc ctcggtaaag gcgtgaagag
    22081 ccggatcgcg ctcgcccttg gcgcggtgca cgcggcccag cagggtccac ccggtctgcg
    22141 tccacccctt atccaccttc aactcgacca gtcgggtcgc ggcctgaatc gccgcagccg
    22201 gctgtttctg ctccagttgg aactcggcaa gccacgccag ccgctcggca ccgagttttt
    22261 cggcgatcgg cgcgtccagc agtttctgga aaaaggaggc ggcctcgggt ttacgcccgg
    22321 tcttctggag cagcatgccc agttccagca tcgactcccg ctcaaagtcc ttggtcggag
    22381 acgccgcgag acaggcacgg aacagcttct ccgcctcggc gtcttcgccg acctgccggt
    22441 aggcggaggc gcgccagaac agaacctcgg ccttgcgggc gtcattcgga aaacggcgca
    22501 tgcgctcatc cagtgtggcg gccgcggcgc gcacgttttt cgcgcgcaac tcctccatcg
    22561 ccttctgata gagcgtctcc gcgacctcgg cgcattcggg ataccgggtc agcagcgcgg
    22621 tccagtcccg caacgccgca tcgctctgtc cgagacgaga caggcagaca ccggaggcat
    22681 agagggctcg ggaggccagc ggatcatccg ggaaacgctg gacgacctgc aagtaccagg
    22741 acgaagccga ctcccatgcc tcctgtttct gcagcagcca ccccagccgg tagagggcat
    22801 ctttgacaag cgggctttgc gggcacttgt ccaccagcag gcggcagtac tgcacggccg
    22861 catcataccg ctgcaacgct aacgccgcct cggcgttcat ccaatagacg tggtcggcgt
    22921 gcaagtcgac cggccgtgtg ttctccgagg tcatcttcaa caccgcctca tacgcgccgt
    22981 catgatacag cgtcgtcagc ttctcatacc cggcctgtcc ggccagccgc cctgttggga
    23041 aggcttcgag ttgttgcgaa tagaccttca cggcctcggc acgccgttcg agttggcgca
    23101 ggcaattcgc ccggaggtag agcacctctt cgcgcgccgg atgtccggcg tcgccgatga
    23161 tcagatcgag actctcaaag gcttcggcat aacgccccgt gtaataattc gcccagccgg
    23221 cggacagcgc gctctcgcgt atgcgcggcg aagcgggata cttcgtcttc aacgtcttga
    23281 acaggttggc gctctcctcg tgccgctggt ccaacagcgc ggtctgggcc gcgaagaaca
    23341 gcgcctcctc cgccactttg ggatctcggg ccttgtgcgc caaatccaga tagatgccca
    23401 tggcacgacg ccgatcctcg ggcttgccgg tcttaccgag cagccacgcg atacgcaatc
    23461 ccgcatacat gccgtagtcg gtctgcgcgt actcccggct taactgttca tagcgcgcca
    23521 gcgcctccgc aggtttgtcg agcttttcaa gcgtctcacc aagatggtac agcgcggccg
    23581 accgcacttc cggcggcatg cccgtcgcgg tcagtttttc aaacagcgcg gccgcaacgt
    23641 tcagttccgc acccccggcc ctgagcagca gcagcgcccg ctgcagatcg gcgcgcgcga
    23701 cattcggact gtcgggaaaa ctttcggaga gttgcttgta ggtcgcggcc gcctctgccg
    23761 cgcggcctgt gcgacggtaa cactcggcca gacggaacag cacattgtcg cgatccggca
    23821 cttgcggcgt ttcggccaag gacacgtatt cgcgcgcggc cagatcaaac aggctgcgcc
    23881 ggaagagccc gtccgccagt tgcagacggt cccgcatctc caccgggtcc gccgcacgca
    23941 cgccgcacac ggcgagcagc agcattccgg cgctaatctg tgcccggcat atcctcatgg
    24001 cgtctccgtg gcggcaggct tctcttcgct cgtggccatc gagaagttcc agatgtccgc
    24061 cttgcggcag gtatccacca ccttgatcag gtcctcgtat cgggtctctt tatccgcccg
    24121 cagcaccacc ggttggcccg ggaagtagcg cgcgagacgg tccagccggg cgcggagcgc
    24181 atccagcgtc agatcctgct ggttgaccga cacgcggccg tcacgggtga tgttgatgat
    24241 gatctcaccc ggcaaccggt ccggcacttt gccgctctgc gccgacggca gcgtgatgtt
    24301 gatctcatac tcccactgcg agaagacgga cgtcgtgatg aagaaaaaaa gcatcaggaa
    24361 aatgatgtcc atcatggacg tcatctgaaa acttggaagc acacccctgc ttttctttcg
    24421 gaagttcatg ccttgtcaat cctgtcccgt aaacggggct ttcatcccca tgccgatgag
    24481 ggcggtcagc acctcggacg acgccgcctc gaggttggcg atctggcggc tcgcccgccg
    24541 ccggaaccaa gcgtaaaaca ccatacaagg aatggcgacg atcaacccga agactgttgt
    24601 cacgatcgcc tgcgacacgc cggccgccaa tactaccggc ttggcgctcg cgatgtcgct
    24661 cgcgaccgag ccgaaagccg tgagcatacc gagcaccgtt ccgagcaggc cgagcaacgg
    24721 cgcgatcgtg gccacatcca acaggaattc cgtctgcccc tgaatcgcgt cagcctgacg
    24781 cgcaccctcg gcctcggccg tgctccggag cagcgcaaca tcgcactgcg gcacattgcg
    24841 cagacagtcc agaaccgcca acaccaccga cgcgagcgga cagggatgac gatcgcataa
    24901 acggcgcacc tccgtcagat cgttggcgcg cacccgcgcc accagatcga tgagcagttc
    24961 gcgcggcgtg atcgccccgg aacgcaacac ggtcatgaag tagaacacca tggccagcgc
    25021 gaagaccgaa atcgcggcca gcacccacat gacccatccg ccatattccc acgcctgttt
    25081 cagcgacatc ccgtatccgt tgatcagggc cggaacagcc gccgcgcttt ccgccgcctc
    25141 ttgcgcccac gcgcacgcca gcgaccccgt gaaggccaaa acccccgtga ccgtttttct
    25201 gttcaccatc ctacccatcc cttctcctgt ttcatcccac accggatctt ccgattccgg
    25261 cacatcatcc gagcgcgtcc gccgcgccgc ccaacagggc actgaagatc gcccgtagcc
    25321 gcgtgcgccg cacacgcacc caacgccgca catcgcgcac acgcgcacgg tatccgccaa
    25381 aatccagtcc cgccgtggct cccagatgcg tcttcgccgc caacgcccgg tccggatccg
    25441 cttcctgcaa cgtttccaga tgggaccgga catgctgata cgcgtcgcgg aacggcatgc
    25501 cgcccgccac cagttccaac gcgcggtcgg tcgcgaacac gtctggcgtg aaccccgcgc
    25561 gcaaccgctc gggacgaacc gtcagcgtac ccaccatcag ccgcatgatg cgcaacgcgg
    25621 cgcgtgtgac cgtcaacccc tccatgtaca acgccttggt ctcctgcaaa tcacggttgt
    25681 agccgcccgg caacgccttc agaatcatgt tcgccgcact gccgtatccg agcaccaccg
    25741 ccgccttggc gcggatcagt tccagcacat ccggattgtt cttctgcggc atgatgctgc
    25801 tgcccgtgca gagttccggc ggcaacgtga aataaccgaa ctccggcatg gagaagagga
    25861 tcagatcctc cgccatccgg gagagcgaaa gcatcaactg gccgcatgcc gccagcaaaa
    25921 ccgcctcgtt cttgccgcgc gcgttgctgg cgtgaaagac attgtggtgc ggcgccgaaa
    25981 agcccagcaa ccgcgcggtg agttcgcgat ccaccggcaa cggcacccca taccctgcgg
    26041 ccgagcccag cggacagcgg tcattgtacg tgtaggcggc ctcgaccatc agcagatcat
    26101 ccaacagggt ctcggcataa gccgtggccc agagccccac cgaactgggc atcgcaggct
    26161 gcatgtgggt ccgccccacc atcgggacct tcgcatgacg gcaaccgaaa cggatcaact
    26221 cccccgccac ttccgcaacc tcttccgtca gccccaacaa ctggtcgcgc acatgcagcc
    26281 ggatatccac cgccacttga tcgttgcggc tccgccccgt gtggatcttg cgccccaatt
    26341 cgcctaaacg ctcagtgaga cggcgctcga cagccagatg aacatcctga tccgcctgcg
    26401 tgatacggaa ctcgccggcc cggatttcgc ggatcacttc cgccagagcc gctatcaccc
    26461 gattacgttc cgctgtcccc aacacggcgg gacgaacgcg catccgggaa agcatggtga
    26521 cgtgcgccgc cgtgcccaaa cagtcccata ccgccagatc gagatccagc accggatcct
    26581 tcccgaccgt gaacgctaaa acgtcaggat gtatcttgcc cacaatcgtc ttctggcttg
    26641 ccgtctcttt tttcatgtgc cgcaacacct ttattcgcgc actctccgcg gccctgctcc
    26701 cgtagcccgt ccggcgtccg cgcgctgcaa aaccattccg cgcgcggtcc ggagttccaa
    26761 ctctctgcgc atcccctccg cctccatcaa tcgcctcaag agttcgccct gcgcctcacc
    26821 agaacacact ccttgaagaa aagaggcata cgcctccgaa acagcaagaa gcataccatc
    26881 ccgaccaacg gcagccatct caatcagacg ggcctgaact gcggcgcggc gctgaacctc
    26941 cggctcagcg gaacgcatcg ccgcctcacg aaaagtcagg taaggccgtt cataacccca
    27001 agttgtacca taaaacgcgg ggtagagcaa taagcgcgac cggaaccgcc gaacaatgcc
    27061 tgaactcgta agcccgggct tgactaccgt gcgccccaag gccgtgaacc agaggtccaa
    27121 cgcggcgtcg aaagcaagag ggtcctctgt tccggtcaga atccgcgcca ccgcatcagg
    27181 cctccactcc tgtccctctg ccgccgcgca caacaacttc tcaaagggca ggccatccgg
    27241 acgccgctcg agcagccaac tcgccagcac cgccgccaca gccggttcgc gcgatgcggc
    27301 gacactctcc atcgcaaaca gcgccgccgc cggaggcagg caggcccgcg accacaacag
    27361 cagcgtgcgg tcaatgtcct gctgccgcgt ctcccggccg gtgtggcgca ccacgccgtc
    27421 gaccagccag agcggcacgt cgcgcatcac gccttcgcgt ccgcccgcct ccaccatcca
    27481 tgccctcaac agcgccagcg agatcgcgcg gcggaatcgt cccagatccg ccgcctcggg
    27541 atcgggcaac tcgatccgct cgaacacacc gcccccgggc tcccgcacgc gcgaccccaa
    27601 cacccgcgtg tcgccgtcac ggcggttgcc gatcacgatt tccaacggac acgagggggt
    27661 caccaacttg agccgtgtgg cccgcgccag ctcgtcgcgc gtccggtcca caaacaccaa
    27721 taccggggcg cggtaggacc ccatcgcaac cggagcgcgc accacgatcg aaccgtcccg
    27781 ggaggtgatc gccccggccg gagccgccag ccgtgtgcga cgcggcccgt aaggctcccc
    27841 cccgaccgct gcaaacatgg acagcaccca acctgccgcc gtggccaacc gcctgatccg
    27901 actgtcgtgt cccatacccg cttccgccct tttccgtccg cgttccgacc gcgcacgctt
    27961 gattcgtcac cggtattatg caacactgga gcggctgttt tcaatggaag gtttgcccca
    28021 tgctatactt tgaatgcggc tcgaaatccg gcgcgctcga ccgcacgcga ctgaaacaag
    28081 gactcgttga ggcccttgac cgcctgggtc cgcgccaaaa ggtgttggtg ctgccgccgg
    28141 acttcacccg gttccactcg caggccggcc tgctgaccca gtacgtgtgg gagtattacg
    28201 gcgaccggct ggccgccgtg atgccggcct tggggaccca ttcgccgatg accgaagcgc
    28261 agatcc
//
LOCUS       DAOSRB010000012        27383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1943_length_27383_cov_6.540362, whole genome shotgun sequence.
ACCESSION   DAOSRB010000012 DAOSRB010000000
VERSION     DAOSRB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 27383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27383
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1943_length_27383_cov_6.540362"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            601..1002
                     /locus_tag="PLZ74_01430"
     CDS             601..1002
                     /locus_tag="PLZ74_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97014.1"
                     /translation="MRQGLRVAFFCLAVTVGGGMAGFGQDFSMLSEAERKQVNGWMAE
                     RAAILADAHKIEIELDQAWGNPQFTSPEVEAQRERYRQLQHELMMTQLTLRRLVEKLP
                     AAQSRQSDLDALRNRAEALSRQIEAKGGVKR"
     gene            1049..2590
                     /locus_tag="PLZ74_01435"
     CDS             1049..2590
                     /locus_tag="PLZ74_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOR97015.1"
                     /translation="MTRENKSSTGKNRRPRILVVTPEITYLPSGMGNMACKLNAKAGG
                     LADVSASLVAALFDLGADVHVALPHYRRMFHIDVGQLISDELRVYKSRMPDSRIHLAE
                     DRCFYYRDTVYSNSQDENPKLALAFTREVLNNIIPFVQPDLIHCNDWMTGLVPGAARR
                     MGIPCLFTVHNIHTHHLTLAQVEDSGIDAAEFWANLFYTRVPYNYEESRNTNQIDMQA
                     SGVFASHYINTVSPTFLEEIVNGWHDFIPTALREEIRNKHRAGCAIGILNAPDASDNP
                     ETDPHIEVCYDAATHREAKRANKVAFQHKVGLEEDPDVPLFFWPSRLDPAQKGPQLLA
                     DILYRFMSRHWRQRVQIALVANGVFQKPFHDILHFHEMYGRLAVCDFNQGLSQLGYAA
                     SDFTLVPSLFEPCGLPQMVGQLYGSLPVVHDTGGLHDTVEHLDPAQNQGNGFVFRIYD
                     SRGLDWAMEKAIEFYKCAPEVRERNIARVMREGKARFNHETCARAYIEIYEKMLNRPL
                     VRSFE"
     gene            2626..4638
                     /locus_tag="PLZ74_01440"
     CDS             2626..4638
                     /locus_tag="PLZ74_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOR97016.1"
                     /translation="MPATLRRPRLLDDPYLLPYAEIIRRRAELADRREADLSQGPGKL
                     AEFACAHEYYGLHRTDSGWVFREWAPNATALWLVGDFSSWQCQEPFRLQRIPGRGVWE
                     AHLAPDAVRHGQYYRLEMVWDGGRGERIPAYARRVVQNPETLVFAAQVWDPEPYVWRN
                     PDFRVPDRVPLIYEAHVGMAQEREGVGGYTEFRERILPRIKRAGYNTVQLMAVMEHPY
                     YGSFGYHVSSYFACSSRFGTPEELKALVDAAHGEGLAVIMDIVHSHAVKNEREGLSRF
                     DGTLYQYFHDGLRGWHEAWDSRCFDYGKTDVLHFLLSNCRYWLDEFRFDGFRFDGVTS
                     MLYLHHGLGVDFMDYSQYFDATVDEDAWIYCRLANRVIHQVRPDAMTVAEDVSGMPGL
                     AAPLEDDGAGFDYRMAMGVPECWFRLVRDVRDEDWSIGYLWHELTNRRADERTVNYVE
                     SHDQALVGGKTLFFQLVDAAIYDAMLRSMRHLEVERGVALHKLARLATLATAGHGYLN
                     FMGNEFGHPEWIDFPREGNGFSYRYARRQWTLRDNPELLYWCLGEFDAAMLEMVTRYR
                     ALEGTLPRRLFVSDQEKILVFERGPLVFLFNFHTHTSVADFKVLVPPGSYHWVMDSDE
                     ERFGGQGRIAAGQSFALIAEQRDRELCQTLRVYLPCRTAMVLERRI"
     gene            complement(4655..5503)
                     /locus_tag="PLZ74_01445"
     CDS             complement(4655..5503)
                     /locus_tag="PLZ74_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOR97017.1"
                     /translation="MLRSSYHLCTVFGIPIRLDASLIILMFLLIGNYGNPLYGIAAGI
                     ALLVSITLHELGHSLTAMAFGCRVRDITLMMMGGCATLLSMPRKAWQELLMAAAGPAV
                     SLGLALAGLLLPDVTYRDGHVLFASGWDRFLFLQIGALNAMLFVFNLLPAFPMDGGRM
                     LRAILQQFFMTRLRATWVASRIGRFLALLLGLSVLYSLFTGRFDGFKLIRLLIAYFIY
                     QAAEGEYRMVLAEERAGRATSPFDGFPFMGGRSSAMPPPDDGKAVVSPPPYERRGATR
                     VDVHKT"
     gene            complement(5518..6504)
                     /locus_tag="PLZ74_01450"
     CDS             complement(5518..6504)
                     /locus_tag="PLZ74_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOR97018.1"
                     /translation="MNPSLSHLVFQPVYKRYVWGGDRFRTFFHRTGVPAVCAESWEIS
                     AHPDGMSVVSCGPFAGQTLAELTARYGAALTGTRAPDPARFPLLFKLLDASDRLSVQV
                     HPNAACAARTGGEPKTEAWVVLDCTPGASLYAGFEPQVTPEVLRAALSDGTVAEQLLR
                     FEPQPGQALFLPGGMAHAMGAGCLIYEVQQSANTTYRLFDWNRTGPDGRARPLHIGPG
                     LASIDWSLPPPQLLTPQLPCPTGTAPVWSEIVTSPVFTVRRLDLREPLRVAPDGTTFL
                     ALFAAAGRVTVADAGNRQHATLPPGSSLLLPATADAHILTPHPESTLYVTTL"
     gene            6755..10048
                     /locus_tag="PLZ74_01455"
     CDS             6755..10048
                     /locus_tag="PLZ74_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97019.1"
                     /translation="MRHLPALLAVFCLAVLCLRAGEGEWQRGFLGKAEFNGTLKGSSA
                     ETEWELVFTRKGRALDATGGVHEVNQPIFNREGLRMAGGRETEYSDSSGAITNHAGIF
                     QTREILSLPPVLTQRMPEDDFTVLVTFSVFKTGGLYQTVFEQTRRQRWWPTLHLGFRS
                     QHRNQLTLHHADRTDSSAKRFAETGVLPETGRVYTVVLTRQRGTAKVWVDGVKRAELA
                     GIPVEDVSDWNLGCGGPERALDGHLRTFAAWDRALDEEEVAAIDLNDLATAGLVRVTP
                     LRYIRHLARGFEPRSYSFQVRNLSDTALEGVAFEWEIGDQRFTQPVGRLEAQGERVID
                     VPVLITLKPGDTVQTFSVTAGGKALNRQSLPATVTPAPVPVNQLQTSSWCQFLPGEPG
                     YTMATVRHCADAMLAGLHWGPVQLRWTGWARADHPEDRAFTPHQKRSYNDFYFSERMR
                     EQMRVCTDGIVRRLAFYPALTHIDINNEYNPNFQVNFSEPARRYAAEHFGLDLTPWMG
                     DGKRELVRPIGRLSPVLANYPIPASRIIPASDPLYRWHLDRKGPHGDSESLLNQHIAT
                     AMRRERPDVLSVVAPLTRRLSLRGYDDRLDIAQTWCYTPGLRVVWLQEALTAHIRHQP
                     RMRSSSFPAFLHMNAGPYPAMPSGDIFAASCWASLIRPTSMLVLFQALEMMRGHGLPK
                     AEIDALLGSTAPNAEEAARVIRAKRLSFKGDHPTLAPRVRRLLAEELPPFRALLPAWS
                     NAPRQIAVYSCFADSLFADQRWPTDYTPLLAELTRSGVAYDLLFDEDFTRDPKLLDGY
                     DMVFIPNLTYLFEPAWQALRTFVETPGRVLAVPDTAHAFFPNAVTVRDTGGALSAEEH
                     ARIVAEVESRYGADSGRPAVIEALDARLAEAAAQGADAGPFAPLLDRLGRLNARCLMP
                     HTALNVTRLDDTHFLSVVNDLRTWGPIYGHFRVLPERGVRQTGDVLFRSALGRFAYNL
                     RTARRLAVTTGGNGQSRLSLTLDGGDAAILLLSDHELGRFRAGIVREGTYMRVTAQAC
                     DTTGREIDVSMPCRVAVEGIPDYSGVALFRKGKLDYRFPFDGTFPTVTLREEATGATV
                     TLRTATLRD"
     gene            10175..11464
                     /locus_tag="PLZ74_01460"
     CDS             10175..11464
                     /locus_tag="PLZ74_01460"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR97020.1"
                     /translation="MKRMLIGTAFACALAGMGMAAGVLEEGFVSLFNGKDLSGWTGAT
                     GGYAVENGVLFCKEKSGGKLLTEKQYDNFILRFEFLVQTNSNNGLGIRCPAEGDAAYN
                     GMELQILDDSGPKYTALKPYQYHGSIYGVVPAKRGHQKKVGMWNFQEVRAIGSRITVI
                     LNGEVIVDADLSTINETMDGKPHPGLRNTKGHIAWLGHGSRVDWRHIRIKEVPADYTV
                     ARPTDNRAPEGFTLLFNGKDMTNWKGVTREERFDNPLVRQQATPEKRAAMQKKADELA
                     RKHWHIRDQALYFDGKGYSLATAKDYGDFEMWVDWRLLSVRGDSGLYLRGSPQVQIWD
                     AHNQWNIGSGGLYNNKKNPSKALKIADRLPGEWNTFYIKMVGERVTVKLNGELVVDNT
                     VLENFWDRSKPVFPKEQIELQCHGDPLEFKNIYIREL"
     gene            complement(11542..12717)
                     /gene="nspC"
                     /locus_tag="PLZ74_01465"
     CDS             complement(11542..12717)
                     /gene="nspC"
                     /locus_tag="PLZ74_01465"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOR97021.1"
                     /translation="MPLPTPAFVIDTAALERNLAVLREVKRRTDCRILLALKAFSMFS
                     VFPLLRRTLDGCCASSVHETRLGREQFGGEVHAFAAAFSEGEMSEIATLADCVTLNSL
                     SQWRHFRHILVSVGRDIPCGLRINPEHSEGTTPLYDPCAPGSRLGIRRETLNNERLDG
                     ITGLHSHTLCEQDAAPFQRTLEVIEARFGDLLPQMRWMNFGGGHLITQPGYDLDLLCN
                     TLNAFRERHPQIETLYLEPGAACVLNAGTLAATVLDVVENDMPIAILDASCACHMPDV
                     LEMPYRPRVFRDAGWHGEAVPGPQADCGADPGVKAHTVRLAGPTCLAGDIIGDWSFDS
                     PVRPGDRILFEDMAHYTMVKTNTFNGIRLPTLAVREPDGAIRIARVFGYEDFKSRLS"
     gene            complement(12815..13663)
                     /locus_tag="PLZ74_01470"
     CDS             complement(12815..13663)
                     /locus_tag="PLZ74_01470"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOR97022.1"
                     /translation="MNTPLFEDAFRALPPPCTDGEPDCGMILGSGWSQTLSVDPPIAE
                     IAYDQIPGLGASTVVGHAGRFVLFERHGIRVAAFLGRRHWYEGAGWEPVVLPVELLRR
                     LGTRNLLITNAAGGIRPTFKPGDLMLIRDHINSVGLNPLIGPVLPGWGPRFPDQSRVY
                     DPELTNLLREAAHDSGTSLSDGVYVFTSGPGYETPAEIRAYSGMGGDAVGMSTVPEAI
                     VANAAGMRIAALSCITNMAAGISGPHLSHEEVLEQTRVTAPLMSSLLDAFLARLAHQI
                     RRVPRE"
     gene            complement(13660..15027)
                     /locus_tag="PLZ74_01475"
     CDS             complement(13660..15027)
                     /locus_tag="PLZ74_01475"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOR97023.1"
                     /translation="MTFPVFHGTNTEVRSKLLEARLTVRCAPVYLSGMVNLILERVRV
                     IDPAEGRDETADLVLRDGILQGGAATTEGLRRIRAEGWIAAPGFWDVHVHFREPGNTQ
                     AETRRSGAGAAAAGGFTHVVTMPNTSPAGDSAVWVQEQAADDVPVRILPAACVTRGRQ
                     GRDLAELEALAQAGAVAFTDDGAMVADEDVMRCAMTRARALNRPVMDHAVVPSIAGKG
                     VVRDCPLARANGWPIFPAEAEVEAVRRDIRLCRETGCATHIQHVSCAESVAAIRVARA
                     EGLPVTAEVTPHHLALAAEDIPSDDGHFRMNPPLGTRKDARALREAVLDGTLGLFATD
                     HAPHTPETKTHGFLRAAFGVIGLETAIGVTWRILVEEEGMTPLDWVARWSCGPAALLR
                     QPVPALRPGLPVDLVLIDPATPWIVNPETFRSRSRNTPFAGWNLHARARLTVCGGRAT
                     YSDLP"
     gene            15161..16465
                     /locus_tag="PLZ74_01480"
     CDS             15161..16465
                     /locus_tag="PLZ74_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOR97024.1"
                     /translation="MSKDSVTRETVLAFHIGGKIGMRMSKPLQTQTDLCLAYTPGVAH
                     AVREIATHSAKVFDYTAKRSLVAVVSDGTAILGLGDLGAEASIPVMEGKAVLFKSFGD
                     VDAWPVPLNHCRQGGANTGKTDPQRVIDAVLAIAPMYGGINLEDIAAPACFEIEDRLD
                     ELLDIPVFHDDQWGTAVITLSGLMNYALLSDRSMESLRVVINGAGAAGIRIADMCKAA
                     GVQDLVMCDTRGVIHTGRADLNAHKRRHACDSAARTLQEVLRGAHVFIGVSAADAVTA
                     EDVRGMAEYPAIFAMANPDPEIRPEGVAEVLGDKPYVMATGRSDYPNQVNNVLGFPFL
                     FRGALDVKARTINTAMKVAASEALAALARQPTPSEVQALYPRENLVFGNGYIIPKPFD
                     RRLFTGVPLAVARAAVASGAAPADTDLEALQVELAARDAARG"
     gene            complement(16531..17337)
                     /locus_tag="PLZ74_01485"
     CDS             complement(16531..17337)
                     /locus_tag="PLZ74_01485"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOR97025.1"
                     /translation="MKPLQIGGRLFRSRLFIGTGKFGSHDVMRQAIEASGAELVTVAL
                     RRVDLDKNPAGDPLLSQLDPEKVLIVPNTSGARTAEEAVRIARLARAAGGYDWIKLEL
                     TPNLHHLLPDPIETLKAAEILVREGFRVLPYINADPVLAKRLEEVGTVAVMPLAAPIG
                     TNLGLRTRDMIEIIVSHAQVPVVVDAGLGLPSHAAAAIELGADCVLVNTAIATAADPV
                     AMAQAFALAVRAAEAGLDAGPRAARLDAEASSPLTDFLWQEPEATPDNVR"
     gene            complement(17425..17625)
                     /gene="thiS"
                     /locus_tag="PLZ74_01490"
     CDS             complement(17425..17625)
                     /gene="thiS"
                     /locus_tag="PLZ74_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOR97026.1"
                     /translation="MTVTVNGKPRPLGKAHSLADLLKALGVNAIHMAVERNDVIIPAH
                     AFETTPVAPGDRIEIIQFVGGG"
     gene            complement(17622..18692)
                     /gene="hydE"
                     /locus_tag="PLZ74_01495"
     CDS             complement(17622..18692)
                     /gene="hydE"
                     /locus_tag="PLZ74_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOR97027.1"
                     /translation="MVSLRHLKKMHQGALPSVAELASLLKAQGRVRERIYRAADTLRR
                     ENMGDAVFLRGIIEFSNVCKNNCLYCGIRCGNPHVGRYRMSADEIVNTARQAKHWGCG
                     TVVLQSGEDPFYTTEILCGMIQRIKEETGVAITLSIGVRPRAELARLRTAGCDRFLLR
                     FETTSPALFAAIHPDESFEHRVQCLTDLRDLGYQVGSGFMIGLPGSTPEMIAQDILFA
                     TQLKLDMIGCGPFLSHPGTPLAEKPLLEDRSVYYKTVALLRLLNPQAHIPATTAFDAL
                     EPEGRNGVLMRGANVFMPNATPDKYRRMYQLYPGKPYADEEGATCAMYARERIAAIGR
                     EVDSGPGHARRLTVSADRKECT"
     gene            complement(18837..21521)
                     /locus_tag="PLZ74_01500"
     CDS             complement(18837..21521)
                     /locus_tag="PLZ74_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97028.1"
                     /translation="MRFTGKMTIAVAAVVAGTAAAAEFTIATNGSDRAEGTAAAPFAT
                     FSRARDAVRALRMREPGRAVPVVVEIREGVYELEEPLAFRTQDAGTPEAPLLFMAADG
                     AKPVLSAGRRITGWKTDADGVWRATAPGTARRERAFSQFFVNGRRRLRPAFPENGYFV
                     PEANLPGDTNTRDSRKGFVFARGDIQPSWHHFRDVEVSMIWAWQYARAHVSSIDAAKR
                     EAVLSLDADKLFNSSIRTDIYYRLVNIREGLKRPGQWYHDRETGEIAYIPEQGETPDE
                     AETIASCLENAVVIRGEKGGKHVSDITFRGLTFAYTDWSISTNGYWDSQGAAELPAAI
                     HVDYADRIRFENCAMRNTGGTAVRFGLGCRDCAADGCEMTDIGGGGVASGAFWKGSGD
                     KDNETSGTVVRQCLIGWYGRDQPGAVGILIGGANRCLIAHNTVHDGYYSGISVGWTWA
                     PYPSFTRENTVEWNHIYGYAPGVLSDGGGVYTLGLQPGTVIRHNRVHGAVAARYGFHS
                     FYFDQGSSCLTLSNNLAYDTDSGVVIPSEDKSGNLIVNNIFADGRDQQFYANDRTADC
                     KFGTNRIERNLFVWRGANRGGNTGAPNRYASNLWWQVDSPERIQTAFNGKTFAEWAKT
                     DTGAVFADPQFRNADMGDFTLSPDSPAHALGFVPFDLTDAGRTLPLCFAGDQPLPGET
                     FPVAPLDQHKTLSTGFEECCAGTTKFGLAGWHTEGRNPSNLLVFVTEKNAAKGRHALK
                     MLNDKSIGPTPALRSWGRRLSGRQTHQFDMYLEPGATFEYSILDANVPRRRYAVETAV
                     FRVNGQGDLAGADGKTVMRLPSGTWVHVEMRISLGLAACGAYDLRVCLPHGNSRLVTA
                     IPLPRTLRQIDGFRFAVGGQDKTAVCLDNLDIESDRPR"
     gene            21726..22424
                     /locus_tag="PLZ74_01505"
     CDS             21726..22424
                     /locus_tag="PLZ74_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97029.1"
                     /translation="MSVVQPLLDLQEIDDRIRKLQQEIHDIPQRKAQEQERLTSVQEV
                     LTRAQAELKAAQVRVSEAELEVKARQDKIQSIKQNQVLLKTNKEFQLYNLEIAKLEGE
                     IEAYEARQLAAMDDVIPVRHRVAEAEAKLKEDQAAVDADLTELDARLAAVRETLAAEE
                     GARVEAAKRVTPQFLLYYERLRTKRWPVVVALDEGGVCKGCNLVQPPSVGQMVRRNQG
                     IVPCQMCGRVLYQD"
     gene            22489..22855
                     /gene="rnpB"
                     /locus_tag="PLZ74_01510"
     ncRNA           22489..22855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLZ74_01510"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23236..24372)
                     /locus_tag="PLZ74_01515"
     CDS             complement(23236..24372)
                     /locus_tag="PLZ74_01515"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR97030.1"
                     /translation="MNLIPPTLLHPNRTLAGPDKILHLTQEAAGFGPRGLLVHGASLQ
                     RSGLLDAILAQPPSGMTVHTWCHAGGEPTVHAVEALRTAIRTERPDWVAAVGGGSVMD
                     LAKAAAGLADAPESVTFYMSHASLIPPAPLPLIAAPTTAGTGSEATGVAVLTDPERTL
                     KQSIRHPSYLPRLVILDPRLLRDCPPDTLAAAGLDAFVQAFESYTSRHATPWTRVLSE
                     LALTRIAQNLLPLYRGDADRAAAMLEASFLAGVALSHARLGVVHGLAHPLGVRFHAAH
                     GLACACCLSACLAFNRPVLSDTLRDLRDRLGLDIENQTATWLETMSLANPFVGKSLPD
                     REAVIRETLASGSTAANPRPVSADDVSDLLDAILGHDALPAAPY"
     gene            24544..25824
                     /locus_tag="PLZ74_01520"
     CDS             24544..25824
                     /locus_tag="PLZ74_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOR97031.1"
                     /translation="MSRLRFWNERAETLSRDELTAVQLAGLKWTVNHVWTNNAFYRDR
                     LAAHGIEPGDIRSLDDLRSLPFLTKDDFRDQYPLGMLCMPQDTIREMHMSSGSTGTPV
                     VMAYTEADLTQWAECMARCQVMAGLQPGDAIQIMPTFGLFNGGFGFYHGARLAGMFAV
                     PASSGNTARQIMLLRDFKVRAMGSVVSYAARIMEILDAKQIELPHLKIGIFGAESFSD
                     EMRNRVESRLGIRAFDIYGMTETGGVGTTGMDCEARCGIHVWEDHYLTEVVDPQTGAP
                     VPDGAVGELVFTSLTREAVPVIRYRTRDLSRIVSREPCACGRTSLRIDRITGRCDDML
                     IIKGVNFFPKQVEQALMEIPGVLPEYQIILEECHGVTDVRINVEVEEGVTGYQVVKHL
                     KKRLGFSPKGDVFKPGTLPRGEGKAKRVFREVRG"
     gene            25931..27004
                     /locus_tag="PLZ74_01525"
     CDS             25931..27004
                     /locus_tag="PLZ74_01525"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOR97032.1"
                     /translation="MRYLTYVGCYTDEKKEGIHIFETDSETGAFCPVGLVGGIENAIH
                     LTLNASRTRLYAGLGMPEYGASAQNGAVAAYEVQGDTLRLLNHCPVGVTPPCYVALDP
                     SEQALAFAEYTNAVAGVFELAEDGRFTDAPAVTVQHEGEGPSKPRQDKAHAHCAEVTP
                     DGRYLCVVDLGLDQVRVYDFPERRGGLRERSTLTIRSVGGAGPRHILFHPNGRFAFVL
                     HELNNTISSFRYTGEEFVLVQTVPMLPPEFKAYSKAAAIKLSADGRQLFGSNRGHDSI
                     AAYDLDPDSGRMSLLAISKLVGEFPRDFEFLPGGKFVLLGHENSNELFSYAYDAKSGV
                     FTPAHGPFSLHRPVLVRFGVRRS"
BASE COUNT         5158 a         7918 c         9042 g         5265 t
ORIGIN      
        1 ggtgcaaagt acggggggag catagcgcag cgggcgcggc ctgtcaatgc cgcgccgggc
       61 gcggaattga cgaggaggag ttgaaattta ggatttagga ttttaggatt taggatttag
      121 gagttaggag ttttggggga accgccatga cgccaagaac gccaagtttt tttgggggtt
      181 gagcaggccg cgggtcgcgg gtcgcgggtc gcgggtcaac ctctcttgtt gcggccacgc
      241 agtggctcgc aatcgtgaga gtaaaagcaa ggatactgcc atcgcattgg ttgcagctaa
      301 attcaaaagg ccgctcgtga ttccgatgcg cggctgggtt gccggaggat cgcgtcgcgt
      361 gtacgtgtac ggcacccggg ctcgcggaac gccgggcaag cgtctgcttg cccggacggg
      421 cagacgctcg tcgctgcggg cgagggtccc gtgttcccga ctttctactc gttcccctgt
      481 cttgatttca tgaagtttct tgtgtttcat ggtgcgaaaa gcttctggtt gttgccaaaa
      541 ggtgttccga tgcgtatact gttggcggtt ttatggatac ggacaggaag tggggtaggg
      601 atgagacagg gtttgagggt cgcttttttt tgtctggcgg tcacggtcgg cggagggatg
      661 gcagggttcg gacaggattt ctcgatgttg tccgaggccg aacggaaaca ggtgaacgga
      721 tggatggcgg agcgggccgc cattctggcg gacgcgcaca aaatcgaaat cgaactcgat
      781 caggcgtggg gcaatccgca gttcacttcg ccggaagtgg aggcgcagcg cgagcggtac
      841 cgccagttgc agcacgaatt gatgatgacc cagttgacat tgcggaggtt ggtcgagaaa
      901 ctccccgcag cgcagagtcg gcagtcggat ctggatgcgc tccggaaccg tgcggaggca
      961 ctttcccggc agatcgaggc gaagggcggt gttaagcggt aagcctgtcg cggcatgtgt
     1021 tccgcccgac cccgttcagg aggatgtgat gacaagagaa aacaaatcgt cgaccggcaa
     1081 aaaccggaga ccgcggattc tggtcgtgac gccggagatc acctatctgc cctccggcat
     1141 gggcaacatg gcgtgcaaac tcaacgccaa ggcgggcggg ttggcggatg tgtccgcctc
     1201 gctggtcgcg gcgctgttcg atttgggcgc ggatgtgcat gtggcgttgc cgcattaccg
     1261 tcgcatgttc cacattgatg tcggccagtt gatcagcgac gagctgcgcg tctataagag
     1321 ccgcatgccg gattctcgaa tccatctcgc ggaggaccgc tgcttttact accgcgacac
     1381 ggtctacagc aacagtcagg acgagaaccc caagctggcg ctggccttca cgcgcgaagt
     1441 gctcaacaac atcatcccct ttgtccagcc tgatctgatc cactgcaacg actggatgac
     1501 cggtctggtt ccgggcgcgg cgcggcgcat ggggatcccc tgcctcttta cggtgcataa
     1561 tattcacacg caccacctga ctctagcaca ggtcgaggat tccggaatcg acgccgccga
     1621 attctgggcc aatctctttt acacgcgggt gccgtacaat tacgaggaat cgcgcaacac
     1681 caaccagatc gacatgcagg cgagcggggt ctttgcctcg cactacatca acacggtgag
     1741 cccgactttt ctggaggaga tcgtcaacgg ttggcatgat ttcatcccaa cggcattgcg
     1801 ggaggagatc cgcaacaaac accgtgccgg ttgcgcgatc gggattctga acgcgccgga
     1861 tgcgtcggac aacccggaaa cggatccgca catcgaggtg tgctatgacg cggcgaccca
     1921 ccgtgaggcg aaacgcgcca acaaagtcgc gttccagcac aaggtggggc tggaggagga
     1981 tccggatgtg cctcttttct tctggccgtc acggctcgac cccgcccaga aaggtcccca
     2041 actgctggcg gacatcctct accgcttcat gtcgcgccat tggaggcagc gtgtgcagat
     2101 cgcgctggtg gcgaatggcg tgtttcagaa gccgttccat gacatcttgc actttcacga
     2161 gatgtacggg cggctggcgg tgtgtgattt caatcaggga ctttcgcaac tcgggtacgc
     2221 cgcgtccgac tttacgctgg tgccgtcgct cttcgagccc tgcggcctgc cgcagatggt
     2281 ggggcagctt tacggatcgc tgccggtggt ccatgacacg ggcggattgc atgacacggt
     2341 tgagcacctg gatcccgcgc agaatcaggg gaacggtttc gtgttccgga tttatgacag
     2401 cagggggctc gattgggcca tggaaaaggc gatcgagttt tacaagtgcg cgccggaggt
     2461 gcgcgagcgg aacatcgcgc gggtgatgcg cgagggcaag gcgcggttca atcacgaaac
     2521 ctgcgcccgg gcctatatcg agatctatga gaagatgctg aaccgtccgc tggtgcgttc
     2581 gttcgaatga tgcttccgtc tatctatctg caaggagatt cgaacatgcc agccacatta
     2641 cgccgccccc gcctgctgga tgatccgtat ctcctgccgt atgcggagat catccgtcgg
     2701 cgtgccgaac tggccgaccg gcgcgaggcg gatctgtccc aaggtccggg caagctggcg
     2761 gaattcgcct gcgcgcacga gtattacggg cttcatcgaa ctgattcggg atgggtcttc
     2821 cgggagtggg cgccgaacgc cacggcgttg tggctggtgg gggatttctc gtcgtggcag
     2881 tgtcaggagc ctttccggtt gcaacggatc ccggggcggg gggtctggga ggcgcatctg
     2941 gcgccggatg cggtgcgcca cgggcagtac taccggctgg agatggtgtg ggacggcggg
     3001 cgcggcgaac gtattccggc ttacgcgcgc cgggtggtgc agaatcccga gaccttggtg
     3061 ttcgcggccc aggtgtggga tccggagccg tacgtctggc gcaatcccga cttccgggta
     3121 ccggaccggg tgccgctgat ctacgaggcg catgtcggaa tggcgcaaga gcgggagggg
     3181 gtcggcggct acacggagtt ccgggagcgg attctgccgc ggatcaagcg ggccggatac
     3241 aacacggtcc aactcatggc ggtgatggag catccgtatt acggctcgtt cgggtatcat
     3301 gtgtcgagct acttcgcctg ttcgtcccgt ttcggcaccc ccgaggaatt gaaagcgctg
     3361 gtggatgcgg cgcacgggga agggcttgcc gtgatcatgg atatcgtgca ttcccacgcg
     3421 gtcaaaaacg agcgcgaggg gctgtcgcgt ttcgacggca ccctctacca gtatttccat
     3481 gacggcttgc gcggttggca tgaggcgtgg gattcgcgct gtttcgatta cggtaaaacc
     3541 gatgtgctgc actttctgct ctccaactgc cggtactggc tggatgagtt ccgcttcgac
     3601 ggattccgtt ttgacggggt caccagcatg ttgtatctgc accacgggct cggagtggat
     3661 tttatggatt acagccagta tttcgatgcc acggtggacg aggacgcgtg gatctactgt
     3721 cggctcgcga accgcgtgat ccatcaggtg cggccggatg cgatgaccgt ggccgaagat
     3781 gtgagcggca tgccgggcct tgccgcaccg ctggaggatg acggcgcggg attcgattac
     3841 cggatggcca tgggggttcc cgagtgctgg ttccgtttgg tgcgcgatgt gcgggatgag
     3901 gattggagca ttggatacct gtggcacgaa ttgaccaacc ggcgcgcgga tgagcggacg
     3961 gttaactacg tggaaagcca cgatcaggcg ctggtgggcg ggaaaaccct tttcttccaa
     4021 ttggttgacg cggcaatcta tgatgccatg ctgcgttcga tgcggcatct cgaggtggaa
     4081 cgcggtgtgg ccctgcacaa attggcgcgg ttggccacgc tggcgaccgc tggccacggg
     4141 tatctcaatt tcatgggtaa tgagttcggc catcccgagt ggatcgattt cccgcgcgaa
     4201 ggtaacggat tctcctaccg gtatgcgcgt cggcagtgga cgttgcgcga caacccggag
     4261 ctcctctact ggtgtctggg cgagtttgat gcggcgatgc tggagatggt cacgcggtac
     4321 cgggcgctcg aagggaccct gccgcgtcgg ttgttcgttt ccgaccagga gaagattttg
     4381 gtgttcgaac gagggccgct ggtcttcctc ttcaacttcc acacccacac ttcggttgcc
     4441 gatttcaagg tgttggtgcc gcccggttcc tatcactggg tgatggacag cgatgaagag
     4501 cgtttcggag gccagggccg gatcgcggca gggcagtcct ttgcgctgat cgccgaacaa
     4561 cgtgaccggg agttatgcca gaccctccgc gtctacctgc cctgccggac cgcgatggtg
     4621 ctggagcgca ggatctgaaa gatccgaacc gcggtcacgt cttgtgcaca tcgacgcggg
     4681 ttgcgccgcg ccgctcatat ggcgggggac tcaccactgc cttgccgtca tccggcggcg
     4741 gcatggcgga ggatcggcct cccatgaagg ggaagccgtc aaacggggag gtcgcgcgtc
     4801 ccgcgcgctc ttccgccaga accatgcggt attcgccttc tgcggcctga tagatgaaat
     4861 aagcgatcag caggcggatc agcttgaatc cgtcgaagcg gccggtgaac agtgagtaca
     4921 gcaccgagag gcccagcaac agggcgagga aacgtccgat gcgcgaggcg acccaagtgg
     4981 cgcgaaggcg cgtcatgaaa aattgctgca ggatcgcgcg tagcatgcgt ccgccatcca
     5041 tcgggaaagc aggcaacaga ttgaagacga agagcatcgc attcaacgcg ccgatttgca
     5101 ggaagaggaa ccggtcccaa ccgctcgcaa acagcacgtg gccgtcacgg taggtcacgt
     5161 ccggcagcaa caggcccgcc agggccaggc ccagactgac cgcaggaccg gccgccgcca
     5221 tgagcaactc ttgccacgct ttgcgcggca tcgagagtaa cgtggcacac ccgcccatca
     5281 tcatcagcgt gatgtcccgg acacggcacc cgaaggccat ggcagtcagg ctgtgtccca
     5341 gttcgtgcaa cgtgatagag acgagcaggg cgatgccggc ggcgatgccg taaagcgggt
     5401 tgccgtagtt cccgatcaac aggaacatca gtatgatcag ggatgcgtcc agacgaatcg
     5461 ggataccgaa aacagtgcag aggtggtatg aggatcggag cataagaggg tcagcggtta
     5521 gagggtggtg acgtataggg tggattcagg gtgaggagtc agaatgtgtg cgtcggctgt
     5581 ggctggaagg agaagactgg agcccggagg gagcgtggca tgttggcgat tcccggcgtc
     5641 tgccaccgtc acgcgacccg ctgccgcgaa gagcgcgagg aaggtggtgc cgtccggcgc
     5701 cacacgcaac ggctcgcgca gatccagtcg gcgcacggtg aatacggggg aggtgacgat
     5761 ctcggaccag accggcgcgg tgcccgtcgg acaaggcaac tgcggcgtga ggagttgcgg
     5821 cgggggaagg gaccagtcga tcgacgccaa ccccgggccg atatggagcg gccgcgcccg
     5881 gccgtcaggg ccagtacggt tccagtcgaa caaccggtaa gttgtgttgg cgctctgctg
     5941 cacttcataa atcagacacc ccgcacccat ggcgtgcgcc attcctcccg gaagaaagag
     6001 cgcttgtccg ggttgcggct cgaatcggag aagctgttcc gccaccgtgc catccgacag
     6061 ggcggcgcgc aaaacttccg gggttacctg cggctcgaac ccggcgtaga gtgacgcgcc
     6121 gggcgtgcag tcgaggacga cccacgcttc tgttttaggt tctcctcccg tacgtgcggc
     6181 gcacgccgca ttggggtgca cttgcacgct taggcggtcc gacgcatcga gcagtttgaa
     6241 cagcagcggg aaacgcgcgg gatccggtgc gcgcgtaccg gtgagcgccg cgccgtatcg
     6301 ggcggtcagt tccgccagcg tttgtcccgc gaaaggaccg catgaaacaa cgctcatgcc
     6361 gtccggatga gccgagatct cccaggactc cgcgcacacc gcaggcacac cggtacggtg
     6421 gaaaaacgtc cggaaacggt cgccgcccca cacgtaccgt ttatagaccg gttgaaaaac
     6481 caagtgtgag agagagggat tcattccgtt tgccagtgta gcgggggagg gtcgcgaatg
     6541 caagagagga gccagcagaa cgcgtcggct tacgatccgc gacattcgac ctgcggaaac
     6601 caaagagcgc tgaaaggtca cctgttcttc gttgtcgcgc acgataacgg gaaatcgtta
     6661 gtctgccgca tgggaaatgg ggtatactgt gtggtatccg ggatcgagat cggaagcgtg
     6721 tctggtcagc ggttcggcgg gaggggttct gggcatgaga catttacccg cgttattggc
     6781 tgttttttgc cttgccgttc tgtgtcttcg agccggggag ggggagtggc aacggggctt
     6841 tctggggaag gcggagttca acggcacgct gaagggatca tcggccgaaa cggaatggga
     6901 actggttttc acgcgcaagg gccgggcgct cgatgcgacc gggggggtgc atgaggtcaa
     6961 tcagccgata ttcaatcgtg aagggctgag gatggccggg gggcgcgaaa ccgagtacag
     7021 cgactcgtcc ggggcgatca cgaatcacgc cgggatcttt cagacacgcg agatcttgtc
     7081 gttgcccccg gtcttgacgc agcggatgcc ggaggatgat ttcactgtgc tggttacctt
     7141 ttcggtgttt aaaacaggag ggctctacca gacggtgttc gagcaaacgc ggcgtcaacg
     7201 ctggtggccg acgctgcacc tcgggttccg ttcgcagcac cgcaaccagc ttacgctgca
     7261 tcatgccgac cgtacggatt cgagcgcgaa gcgttttgcc gagaccggtg ttttgccgga
     7321 gaccggacgc gtctacacgg ttgtgctgac ccgtcagcgc ggtacggcca aagtttgggt
     7381 cgacggggtc aagcgggctg aactggccgg gattccggtc gaggatgtga gcgactggaa
     7441 tctcgggtgc ggcgggcccg aacgtgcgct ggacggccat ctccgcactt ttgcggcttg
     7501 ggatcgtgcg ttggacgagg aggaggtcgc agccatcgat ctgaacgact tggccacggc
     7561 cggtttggtc cgtgtcacac cgttgcgcta tatccgccat ttggcgcgcg gcttcgaacc
     7621 ccggtcctac tctttccagg tgcggaatct ttccgacacg gcgctggagg gcgtggcgtt
     7681 cgaatgggag atcggagacc agcggttcac gcagccagtc gggcggttgg aggctcaggg
     7741 cgagcgggtg atcgacgtgc cggtcctgat cacgctgaaa cccggggata cggttcagac
     7801 cttttccgtc actgcgggag gcaaagcgct caaccgccag tcactgcctg ccaccgtgac
     7861 gcctgcgccg gttccggtca atcagctcca gacctcctcc tggtgtcagt tcttgccggg
     7921 cgagccgggt tacaccatgg cgacggtccg gcattgcgcc gacgcgatgc tggcgggact
     7981 ccactggggg ccggttcaat tgcggtggac cggttgggcg cgcgccgacc atccggagga
     8041 ccgggccttc acgcctcatc agaagcgctc ctacaatgat ttttatttca gcgaacggat
     8101 gcgggagcag atgcgtgtct gcacggacgg gatcgtccgg cggctcgcct tctatcccgc
     8161 gctcacgcac atcgacatca acaatgaata caatccgaac tttcaggtca acttcagcga
     8221 gccggcccga cgttacgctg cagagcattt cggcctcgac cttacgcctt ggatgggaga
     8281 cggcaaacgc gagctggtgc gtcccatcgg gcggctctcg ccggtgttgg cgaactatcc
     8341 gattcccgcc agccggatca ttccggcgtc cgatccgctc taccgctggc atctcgaccg
     8401 caaggggccg cacggtgatt cggaaagtct gctcaaccag catatcgcta cggccatgag
     8461 gcgcgagcgg cccgatgtgc tctctgtggt ggccccgctt acgcgtcgtc tctccctgcg
     8521 cggatatgac gaccgtctcg acatcgcgca gacctggtgc tacacgccgg ggttgcgcgt
     8581 agtctggctg caggaggcgc tcaccgcgca tatccggcat cagccgcgca tgcgatcctc
     8641 ctcctttccc gcctttctgc atatgaacgc ggggccctat ccggccatgc cgtccggcga
     8701 cattttcgcc gcgtcctgct gggcttcgct gatccggccc acctccatgc tggttctgtt
     8761 tcaggcgctt gagatgatgc gggggcacgg gctgcccaaa gcggagatcg atgcgctgct
     8821 tggttcgacc gcacccaatg cggaagaagc ggcgcgtgtc atccgggcca agcgtctctc
     8881 ctttaagggc gatcatccca cgcttgcgcc gcgcgtgcgg cgtctgcttg cggaggagct
     8941 tccgcccttc cgggctctgc ttcccgcgtg gagcaacgct ccgcgtcaga tcgcggtcta
     9001 ttcgtgtttc gcggacagcc tgtttgcgga ccagcgctgg ccgaccgatt acacgccgtt
     9061 gctggcggaa ctcacccgtt ccggggtcgc ctatgatctg cttttcgacg aggacttcac
     9121 gcgtgatccg aagctgctcg atgggtacga catggtgttt atcccgaacc tcacgtatct
     9181 gttcgaaccg gcgtggcagg cgctccggac ctttgtggag acgcccggcc gtgtgctggc
     9241 agtgccggat acggcgcacg cttttttccc gaatgccgtg accgtgcgcg acaccggcgg
     9301 ggcgctgtcc gcagaggagc acgcgcgcat cgtcgccgag gtcgaatccc gatacggagc
     9361 ggacagcgga cgtccggcgg tgattgaggc gttggacgcg cggttggctg aggccgccgc
     9421 gcaaggggcg gacgcggggc cctttgcccc gttactggac cggctcgggc ggttgaacgc
     9481 ccgttgcctg atgccgcaca cggcactgaa cgtgactaga ctggacgaca cgcacttcct
     9541 ttcggtggtc aacgacttgc gtacatgggg gccgatatac ggtcatttcc gtgtgttgcc
     9601 ggaacgggga gtgcggcaga ccggcgacgt gctgttccgc agcgcgctcg gccggttcgc
     9661 ttacaacctc cgcaccgcgc ggcgtcttgc ggtcacgacg gggggtaacg gacagtcgcg
     9721 gctatcgctc acgctcgatg gcggtgacgc cgccatcctg ctgctgagcg atcacgagtt
     9781 ggggaggttc cgtgccggga tcgtgcgcga ggggacatac atgcgcgtca ccgcgcaggc
     9841 ctgcgacact acgggccgcg agatcgatgt ctccatgccg tgccgggtcg cggtcgaggg
     9901 cattccggac tactccggcg tcgcgctttt ccgcaagggg aaattggact accgttttcc
     9961 cttcgacggt acgttcccga cggtcaccct ccgtgaggag gccactggcg cgaccgtcac
    10021 cctgcggacc gcgacactgc gcgattaagg cgctttcgaa gagaacgctg gggtttcagg
    10081 attgctttta cgatcagaaa ggattttaat actcgccgta caagtgcgcg gatggtttct
    10141 gacgcaccgg atacgtatga gggaaaggga gaatatgaaa cggatgttga tcggcaccgc
    10201 attcgcgtgt gcgctggcgg ggatgggtat ggcggccgga gttctggaag agggcttcgt
    10261 ctcgctcttc aacgggaaag acttgtccgg gtggactggt gcgacaggcg ggtacgcggt
    10321 cgagaacggg gttctgttct gcaaagaaaa gagcgggggt aagctgctga ccgagaagca
    10381 gtacgacaac ttcatcctgc gttttgaatt tcttgtccaa accaactcca acaacggact
    10441 cgggatccgc tgcccggcag agggagacgc cgcatacaac ggcatggaac tgcaaattct
    10501 ggatgattcg ggccccaagt acacggcgct caagccgtat cagtatcacg gctcgatcta
    10561 tggggtggtg ccggccaagc ggggacacca gaagaaggtg ggcatgtgga acttccaaga
    10621 ggtgcgcgcc atcggttcgc ggatcacggt gatcctgaat ggggaagtga ttgtcgatgc
    10681 ggacctctca accatcaacg agacgatgga cggcaagccc catcccggcc tgcgtaatac
    10741 caaagggcat atcgcctggc tcgggcacgg ctcgcgtgtg gattggcgcc atatccgcat
    10801 caaggaggtc ccggccgatt atacggtggc ccggccgacc gataaccgcg ctccggaagg
    10861 gttcaccctg ttgttcaacg ggaaagacat gaccaactgg aagggggtca ccagagaaga
    10921 gagattcgac aatcctctgg tccggcaaca ggccacaccg gagaaacgcg cggccatgca
    10981 gaaaaaggcg gacgagctgg cacggaagca ttggcatatc cgggatcagg ccctgtattt
    11041 cgacggcaag ggatacagcc tcgcgaccgc caaggattac ggcgatttcg aaatgtgggt
    11101 cgactggcgt ctgctgtcgg tccgcgggga tagcggactc tatctgcgcg gctcgccgca
    11161 agtgcagatt tgggacgccc acaatcagtg gaatatcggt tccggggggt tgtataacaa
    11221 caaaaagaac cccagcaagg cgttgaagat cgcggaccgg ctgcccggcg aatggaacac
    11281 cttttacatc aaaatggttg gcgagcgcgt gacggtcaaa ctgaacggcg agttggtggt
    11341 cgacaacacg gtgctggaga acttctggga ccgtagcaaa ccggtcttcc ccaaagagca
    11401 gatcgagttg cagtgccacg gcgatccgct ggagttcaag aacatctata tccgcgaact
    11461 gtaaccggtt ccgcttggat agcgcattgg ggacgtgtcc ggaaacggac acgtccttct
    11521 tggtgtgggc cagcgtgaaa atcaggagag ccgggatttg aaatcctcgt acccaaacac
    11581 gcgtgcgatc cggatcgcgc catccggttc gcgcacggca agcgtcggga gccggatgcc
    11641 gttgaaggtg ttggttttca ccatggtgta atgggccatg tcttcgaaga ggatgcggtc
    11701 gccgggtctg accggcgaat cgaaggacca atccccgatg atgtcgcccg caaggcaggt
    11761 ggggcccgcc agccgtacgg tgtgtgcttt gactccgggg tccgcgccgc agtcggcctg
    11821 tggccccggc acggcttcgc cgtgccagcc cgcatcgcgg aacacgcgcg gacggtaggg
    11881 catttccagc acgtccggca tgtggcaggc gcacgaggcg tcgagaatcg cgatcggcat
    11941 gtcgttctcc accacgtcca gcacggttgc ggccagggtt ccggcgttca gcacgcacgc
    12001 cgcgcccggt tccagataga gggtttcgat ctgggggtgg cgctcgcgga aggcattgag
    12061 cgtgttgcag agcaggtcga gatcataccc cggctgggta atcaaatgac caccgccgaa
    12121 gttcatccag cgcatctggg gcagcagatc gccgaagcgg gcctcgatca cctcaagggt
    12181 acgctggaag ggggcggcgt cctgttcaca aagcgtatgg gagtggagtc ctgtgatgcc
    12241 gtcgagccgt tcgttattca gggtttcgcg tcggatgccc agccgggaac cgggtgcaca
    12301 cggatcgtac aggggcgtgg tgccctccga gtgttccgga ttgatgcgca gtccgcaggg
    12361 gatatccctc ccgactgaga ccagtatgtg acggaagtgc cgccactgtg acagggagtt
    12421 gagcgtcaca caatccgcaa gcgtcgcgat ttcagacatc tccccttcgg aaaacgcggc
    12481 ggcaaaagcg tggacctcgc cgccgaactg ttcacgtccc agtcgcgtct cgtgcacgga
    12541 gctggcgcaa cagccgtcca gcgtgcgccg caacagcgga aacacgctga acatggaaaa
    12601 ggctttcagc gcgagcagga tgcggcagtc cgtgcggcgc ttgacttcgc gcagcaccgc
    12661 gagattccgc tccagtgcgg cggtatcgat cacaaatgcg ggtgtgggaa gaggcattca
    12721 ggtctccaga aaggtgttgg gttgggtgac tggcgaatcg aaatcagcaa tacgaaacgg
    12781 attgtttcct taggcagaca gacggccgat gcgctcattc acggggcacg cgccggattt
    12841 ggtgcgcgag ccgcgctaga aacgcgtcga gcagtgacga catcagcggt gccgttacac
    12901 gggtctgttc gagcacctcc tcatgcgata ggtgcggccc gctgatcccc gccgccatgt
    12961 tggtgatgca gctcagcgcg gcaattcgca ttccggccgc attggcgaca atcgcctctg
    13021 gaaccgtgga catccctacg gcgtccccgc ccatgccgga atacgcacgg atctcggccg
    13081 gggtttcgta tcccggtccg gacgtgaaca catacacccc gtccgaaagg gacgtgccgc
    13141 tatcgtgcgc cgcttcacgc agcaggttcg tcaactccgg atcgtacacg cgcgactggt
    13201 cggggaaccg cggcccccag ccgggaagga ccgggccgat cagcgggttc agtcccacgc
    13261 tgttgatgtg gtcacggatc agcatgaggt cgcccggttt gaacgtgggg cggatcccgc
    13321 cggccgcatt ggtgatcagc aggtttcgcg tgccgagtcg acgcaacagt tcgaccggca
    13381 gcacaaccgg ttcccatccc gccccctcat accagtggcg gcggcccaga aaagcggcca
    13441 cgcggatccc gtgacgctcg aacaacacga atcggcctgc gtgaccgacg accgtactgg
    13501 caccgagccc cgggatctgg tcgtaggcga tctccgcgat cggcgggtct acggacaaag
    13561 tctggctcca accggaacca aggatcattc cgcaatcggg ttccccgtcc gtgcaggggg
    13621 gcggcagggc gcggaaggcg tcttcaaaca gtggggtgtt catggcagat ccgaataggt
    13681 tgcgcgtccg ccgcaaactg tgaggcgggc gcgggcgtgg aggttccaac ccgcgaacgg
    13741 tgtgttgcgg gaacgagaac ggaaggtttc gggattcaca atccagggcg tggccggatc
    13801 gatcagcacc agatcaacgg gcaggcctgg ccgcaatgcg ggcacgggtt gccggagtaa
    13861 cgccgcagga ccgcaactcc agcgcgcgac ccagtccaag ggcgtcatcc cctcttcctc
    13921 aaccagaatt cgccaggtga cgccgatcgc agtctccagt ccaatgactc cgaaagcggc
    13981 gcggagaaac ccgtgtgttt tcgtttcggg tgtatgcggg gcatggtcgg tcgcgaaaag
    14041 gccgagcgtg ccgtccagca ccgcctcacg cagcgcgcgc gcatccttgc gggttccgag
    14101 aggggggttc attcggaagt ggccgtcatc agacgggata tcctcggccg ccagcgcgag
    14161 atgatgcggc gtgacttcag cggtcacggg caggccctct gcgcgcgcca cccgaatggc
    14221 ggccaccgat tcagcgcagg agacgtgctg gatgtgcgtg gcgcagccgg tttcccggca
    14281 gagacggata tcgcgccgca ccgcctccac ttccgcttcg gccggaaaga tcggccaacc
    14341 gtttgcgcgt gcgaggggac agtcgcgcac aacccccttc ccggcgatcg acggaacgac
    14401 agcgtgatcc atcacaggcc tattcaacgc gcgggcgcgt gtcatggcgc acctcatcac
    14461 gtcctcgtcc gccaccatgg cgccgtcatc ggtgaatgcg accgctcctg cctgcgcgag
    14521 cgcctccagt tccgcaagat cgcggccctg acggcctcgt gtcacgcagg ccgcagggag
    14581 gatccgcacc ggcacatcat cagcggcttg ctcctgcacc caaaccgcgg aatcgcctgc
    14641 gggcgaagtg ttgggcatgg tgacgacatg cgtgaaccca cccgcggccg ctgctccggc
    14701 accggaacgc cgggtttcag cctgcgtgtt accgggctcg cggaagtgca catggacatc
    14761 ccaaaacccg ggtgccgcga tccagccttc ggcccggatg cgccgcaacc cctccgttgt
    14821 cgcggcaccg ccctgtagaa taccgtcgcg aagaacgaga tccgcggtct catcccggcc
    14881 ttctgccgga tcgatcaccc gcacacgctc taaaatcagg ttcaccatgc cgcttaagta
    14941 taccggggca caccgcacgg tcaagcgtgc ttccagcaat tttgaacgca cttccgtatt
    15001 cgtgccatga aacacaggaa acgtcatgaa atcaagctag ggatatgctc gcgtctgcgg
    15061 cggcagccgc tttcgactcg catgcggtca gggaaacaag taagctatgc ctccattccg
    15121 gatcagtgtg ccggaacgca agcatgcgag gagatggatg atgtcgaagg attctgtgac
    15181 acgcgagacg gtgttggcat ttcatatcgg cgggaagatc ggaatgcgga tgtccaagcc
    15241 gcttcaaacc caaaccgatt tgtgtctggc ctacacgccg ggtgtggcgc acgcggtgcg
    15301 cgagatcgcc acccattccg caaaagtgtt cgactacacg gcgaagcgca gtctggtggc
    15361 cgtcgtgtcg gatggcacgg cgatcctcgg tctcggtgat ttgggtgcgg aggccagtat
    15421 cccggtgatg gagggcaagg ccgtgctctt caaaagtttc ggcgatgtgg atgcctggcc
    15481 ggttcctctg aaccattgcc gacagggtgg cgcgaacacc ggcaagaccg atccgcagcg
    15541 cgtgattgac gcggtgctgg cgatcgcgcc gatgtacggc gggatcaatc tggaggatat
    15601 cgcggcgccg gcctgctttg agatcgagga tcggctggac gagttgctgg atatccccgt
    15661 gtttcatgat gatcaatggg ggacagcggt catcacgctt tccgggttga tgaactatgc
    15721 cctgctttca gatcgctcaa tggaatcgtt gcgcgtggtg atcaacggcg cgggggccgc
    15781 agggatccgc atcgccgaca tgtgtaaggc ggccggggta caggatcttg tcatgtgcga
    15841 cacccggggc gtgatccaca cgggccgtgc cgacctgaac gcgcacaagc gtcggcatgc
    15901 gtgcgacagt gcggcgcgta cgctgcaaga ggttctgcgc ggcgcgcatg tgtttatcgg
    15961 cgtttcggcg gcggacgcgg tgacggcgga ggatgtgcgc ggcatggcgg agtacccggc
    16021 gatctttgcc atggccaacc cggaccccga gatccggcct gaaggggtgg ccgaggtgct
    16081 gggcgacaag ccgtatgtga tggcgaccgg acgatccgac tatcccaatc aggtcaacaa
    16141 tgtgttgggt tttccgttcc tgttccgcgg agcgctcgat gtgaaggcgc gtacgatcaa
    16201 caccgcgatg aaggtggccg cctccgaggc gcttgccgcc ttggcacggc agccgacgcc
    16261 gtcggaggtg caggcgttgt atccgcgcga aaatctggtg ttcggcaacg gatacatcat
    16321 tcccaagccg ttcgaccgcc ggttgttcac cggggtgcct ttggcagtgg ctcgtgccgc
    16381 ggtggcctcc ggggctgcgc ctgccgatac tgatttggag gccctgcagg tcgaactcgc
    16441 tgcgcgcgat gcggcccgcg ggtgatcggt gaacgtgagg tgcggcacgg tacgccatgc
    16501 cgtttcattt ttccctttga tccgagcgcg ttagcggacg ttgtctggcg ttgcctcggg
    16561 ctcttgccac aggaagtccg tgagcggcga cgaggcttcg gcatcgagtc gcgcggcacg
    16621 cggtccggcg tcgagtcccg cttcagcggc gcgcacggcg agcgcaaagg cttgcgccat
    16681 ggccaccggg tcggcggccg tggcgatcgc cgtgttgacg agcacgcaat ccgcgccgag
    16741 ttcgatcgcg gccgcagcgt gcgagggcag cccgagtccg gcgtccacca caaccggcac
    16801 ctgcgcgtgg gacacgatga tctcgatcat gtcgcgggta cgcagtccca gattggtgcc
    16861 gatcggtgcg gccaacggca tcacggccac cgtgccaacc tcttcgagcc gtttggcgag
    16921 gaccgggtcg gcattgatgt acggcagaac ccggaaaccc tccctcacga gaatttcggc
    16981 ggctttcagc gtttcgatgg ggtcgggaag caagtggtgc agattgggtg tcagctccag
    17041 cttgatccag tcgtacccgc ctgcggcacg cgccagccgg gcgatgcgca ccgcctcttc
    17101 cgcagtgcgg gcgccgctgg tattgggcac gatcaggact ttctccgggt cgagttgtga
    17161 gagaagcgga tcccccgcag gatttttatc gaggtccaca cgtcgcagcg ccacggttac
    17221 cagttccgcg cccgaggcct cgatggcctg ccgcatcacg tcgtgcgagc cgaacttgcc
    17281 ggttccgatg aagagacgcg aacggaaaag gcgtccgccg atctggagag gtttcatagg
    17341 cttgtcgttc ccgaggggtt atgaacaggg gaggggtgcg aaacgtcgcc gcgcgcaacg
    17401 gcgcggcagc gtgttttccg ggtatcagcc tccgcccacg aactggatga tctcgatccg
    17461 gtcgcccggc gcgaccggtg tggtttcgaa agcgtgtgcg gggatgatga catcgttgcg
    17521 ttccactgcc atgtggattg cgttgacccc cagagccttc agcaggtcgg ccaaggaatg
    17581 ggccttgccg agcggacgcg gtttgccgtt gacggttacg gtcatgtgca ctccttccga
    17641 tcggcggaca cggtgagtct tcgtgcatgt ccggggccgg agtccacctc gcggccgatc
    17701 gccgcgatac gctcccgtgc gtacatcgcg catgtggcac cctcttcgtc ggcataaggt
    17761 ttacccgggt atagttgata catccgacgg tacttatcgg gggtcgcgtt cggcatgaac
    17821 acattcgcgc cgcgcatcag gacaccgttg cggccttcgg gttcgagcgc gtcgaacgcg
    17881 gtggtggccg ggatatgcgc ctgcgggttg agcagacgca gcagcgcgac tgttttgtaa
    17941 tagacgctgc ggtcttccag caacggtttt tccgcaagcg gggtgccggg gtgtgagagg
    18001 aagggaccgc acccgatcat gtccagcttg agctgcgtgg cgaacaggat gtcctgcgcg
    18061 atcatctccg gggtagagcc gggcaggccg atcatgaatc cgctgccgac ctggtaaccg
    18121 aggtctcgca ggtcagtcag gcactgcacg cggtgttcga acgactcatc cgggtggatc
    18181 gcggcgaaca gtgcggggga ggtggtttcg aaacgcagca gaaagcggtc acacccggcg
    18241 gttcggagtc gggccaattc tgcgcggggg cgcacaccga tggacagggt gatcgccacc
    18301 ccggtctcct ccttgatgcg ttggatcatg ccgcagagaa tctcggtcgt ataaaagggg
    18361 tcctcgccgg attggagcac gaccgtaccg catccccagt gtttggcttg gcgcgcggta
    18421 ttcacaatct cgtcggcgga catgcggtag cgcccgacat gcgggttgcc gcaccggatg
    18481 ccgcagtaga gacagttgtt tttgcagacg ttggaaaact cgatgatgcc gcgcaagaaa
    18541 accgcgtccc ccatgttttc ccgtctcagc gtgtcggcgg cccggtaaat ccgttcacgg
    18601 acccggccct gggccttcag cagagatgca agttccgcga cggatggcag ggcaccctga
    18661 tgcattttct tgagatgacg gagagaaacc atgacataga agtataccgc aaaaggggaa
    18721 cgacgggaag cacgtccttt cgtggacggg cgcgtccgcg gttatcgcat tcttttgttt
    18781 ctggtttcgg ctgtttcgct tttcgcagtg acgcttgagc gggacctttc atcgggttat
    18841 cggggacggt cgctttcgat atcgagattg tcgaggcaca ccgctgtctt gtcttgaccg
    18901 ccgacggcga accggaaccc gtcgatttgc cggagcgtgc ggggcagggg aatggccgta
    18961 acgaggcggc tgttcccgtg cgggaggcag actcggagat catacgcgcc gcacgcggcg
    19021 agaccgaggc tgatgcgcat ttcgacatga acccaggttc cggaaggaag ccgcattacg
    19081 gttttcccgt ccgcacccgc aaggtcgccc tgtccgttca cccggaaaac cgccgtttcc
    19141 accgcatatc tccggcgcgg cacattcgcg tccaaaatgg aatactcgaa cgtggcaccc
    19201 ggttccagat acatatcgaa ctgatgggtc tgccgtcccg agagccttct tccccacgaa
    19261 cgcagggccg gtgtggggcc gatcgacttg tcgttgagca tcttgagcgc gtggcggcct
    19321 ttcgcagcgt tcttttccgt aacgaacaca aggagattgc tcggattccg gccttccgtg
    19381 tgccagcccg ccagaccgaa ctttgtggtg cccgcgcaac actcctcaaa gccggttgag
    19441 agtgttttgt gctgatccaa cggggcaacc gggaacgttt cacccggaag gggttggtcg
    19501 cccgcgaaac agagcggcag ggtccgcccc gcatcggtga ggtcaaaggg aacgaatccg
    19561 agcgcatgtg ccggggagtc gggggaaagc gtgaagtccc ccatgtccgc atttcggaac
    19621 tgcgggtcgg caaagaccgc gcccgtgtcg gttttcgccc actcggcgaa cgttttcccg
    19681 ttgaaggcgg tttggatcct ctcgggagaa tcgacctgcc accacaaatt ggaggcgtac
    19741 cggttcggtg cacccgtgtt gccgccccgg ttcgccccgc gccagacgaa aaggttgcgc
    19801 tcgatgcggt tcgtgccgaa cttgcaatcc gctgtccggt cgttcgcgta gaactgctgg
    19861 tcgcgcccgt ccgcgaaaat gttgttcacg atcaggttcc ccgacttgtc ctcactcgga
    19921 atcacgactc cgctgtcggt gtcatacgcg aggttgttcg agagggtgag gcaggaagaa
    19981 ccttggtcaa agtaaaagct gtgaaacccg taccgtgcgg caaccgcgcc gtggacgcgg
    20041 ttgtggcgga tcaccgtgcc cggctggagc ccgagggtgt agacgccacc cccgtcggag
    20101 agcacgccgg gcgcataacc gtagatgtgg ttccactcga ccgtgttttc gcgcgtgaaa
    20161 ctcgggtacg gcgcccatgt ccagccgacg gagatcccgc tgtaatagcc gtcatgaacc
    20221 gtgttgtgcg cgatgagaca ccggtttgcg cccccgatca gaatcccgac cgcgccgggc
    20281 tggtcccgtc cataccatcc gatcaggcac tggcggacga ctgttccgga ggtctcgttg
    20341 tccttgtcgc ccgaaccttt ccagaaggca cccgatgcca cgccgccacc gccgatgtcc
    20401 gtcatctcgc agccatcggc cgcgcaatcg cggcacccca atccgaaccg cactgccgtg
    20461 ccccccgtgt tccgcatggc gcagttttcg aaccggatgc ggtctgcgta gtccacgtga
    20521 atcgcggcag ggagttcggc cgcgccttgg gagtcccaat agccgtttgt actgatggac
    20581 cagtcggtgt aggcaaacgt caggccgcgg aaggtgatgt cgctgacgtg ctttcctcct
    20641 ttttcccccc ggatcacgac tgcgttttcc aggcacgagg cgatggtttc cgcctcgtca
    20701 ggagtctcgc cctgttccgg tatgtaggcg atttcgcccg tctcgcggtc gtggtaccac
    20761 tgtcccggac gtttcaaccc ttcccggatg ttgaccaagc ggtagtaaat atccgtgcgg
    20821 attgaagagt tgaaaagctt gtcggcatcc agagaaagca cggcttccct tttcgcggca
    20881 tcaatggatg aaacgtgggc gcgggcatat tgccacgccc aaatcatgct cacctccacg
    20941 tctcggaaat ggtgccacga gggctggatg tccccgcggg cgaagacaaa ccccttcctg
    21001 ctgtcgcgcg tattggtgtc gccgggaaga ttcgcctcgg gcacgaagta gccgttttcc
    21061 ggaaaagccg ggcgcagtct gcgtcgaccg ttcacaaaaa actgcgaaaa ggctctttcc
    21121 cttcgtgcgg tacccggcgc agtcgcgcgc cagacaccgt cggcgtccgt tttccatccg
    21181 gtgatccggc gtcccgcact gaggacgggt ttcgccccgt ccgcagccat gaaaagaagc
    21241 ggggcctccg gcgtacccgc gtcctgcgta cggaaggcga gcggctcttc caactcgtac
    21301 acaccctcgc ggatttcgac cactacgggt accgcacgcc ccggttccct cattcgcaat
    21361 gcccgtacgg cgtcacgcgc ccgtgaaaac gtcgcgaacg gcgctgccgc tgttccctcc
    21421 gcccggtcac tgccgttggt tgcgatcgtg aattccgcgg ccgcggccgt gcccgccaca
    21481 acggcggcca ccgcgatggt catcttgccg gtaaaacgca tccgttctcc ctttccccgt
    21541 gtttgttggg cttcctttgt gatttcattg tgatcataac tgtggaggga tgattacgtc
    21601 gagcggaaaa gcggaacggc ggacggcaaa aaaaagcgat ctgtgtttga ctccggagag
    21661 gggatcgggt agtctacatg accatacgtg atctttatca gttgtatccg gaaggaagtg
    21721 ccgcaatgag tgtcgttcaa ccattgctcg acctgcagga gattgatgac cgcatccgga
    21781 aactccagca ggaaatccat gacattccgc agcgtaaggc ccaggagcag gagcgtctta
    21841 ccagcgttca ggaagttctg acccgagcgc aggcggagtt aaaggccgcc caagtgaggg
    21901 tcagcgaggc ggagctggag gtcaaggccc gtcaggacaa gatccagtcg atcaaacaga
    21961 accaggtctt gttgaagaca aacaaggaat tccaattgta taatctcgag atcgccaaac
    22021 tcgaggggga gatcgaagcg tacgaggcgc gccaactggc ggcgatggac gatgtgatcc
    22081 cggtccggca tcgtgtggcc gaggcagagg cgaagttgaa agaggatcag gcggccgtgg
    22141 atgcggatct gacggagttg gacgcccggt tggccgcagt gcgcgagacg ttggcagcgg
    22201 aggagggcgc gcgggtcgag gcggccaaac gggtcacgcc gcaatttctg ctctattacg
    22261 agcgtctccg caccaagcgc tggccagtgg tggtggcgtt ggacgagggc ggtgtgtgca
    22321 aagggtgcaa tctggtccag cctccctccg tggggcagat ggtccggcgc aatcagggga
    22381 tcgttccctg tcagatgtgc gggcgcgttt tgtatcagga ttgatccgga aagaaagcgg
    22441 atgcgtgccg cgccgctgcg ggcggcatat tgcgatttgc ggtgggtgca aggggagtga
    22501 ccggtcgctc ctgccatgtg caggggagga aagtccggac tccgcagggc gagaggtccg
    22561 gccgtgaagg ccggagaggc actgccagat cggtgccgac ggaaagtgcc acagaaaaca
    22621 aaccgtcccg tttcggcggg ataagggtga aaaggtggtg taagagacca ccggacggac
    22681 gcggaagcgc cgtcgcaggg caaaccccct ctggagcaag atcaaatagg ggactcgaga
    22741 cgcggcccgt gtcgttccgg ttcgccggat agagttccgg gtagattgct cagatgaatg
    22801 atcggagccg ctttcgggcg gtgaacagaa tccggcttat tcgcaccctt gtgcccgccg
    22861 cactcttacc ctatacccgt gccacacctg tgaacaaaag gttgcgggct ccgtggggca
    22921 tccccacccg taacgctaga tcagtcgtcc tgccaacgaa cccaaacgcc gcaagtcgaa
    22981 tgtcgcgggt caactctctt tggtttctgg tttcatggtt tcaaggtttc atggtttcat
    23041 ccaaatcggg gtcgggatcg ctatcgctat cgggtttcaa ggtttctggt ttcaaggttt
    23101 catggtttca tccaaatcgg ggtcgggatc gctatcggga tcgggatcga tcatttaacc
    23161 acggaatacc accacctcac agcctaacag cctcacagtc tcacagcctt tgagattgcg
    23221 ggcttcgccc gcatctcaat acggtgcggc ggggagcgca tcgtggccga ggatcgcgtc
    23281 gagtaagtcc gagacatcgt cggctgaaac cgggcgcggg tttgcagcgg ttgagccgga
    23341 ggccagggtt tcacggatga ccgcctcgcg atccggaagc gacttgccga caaaggggtt
    23401 tgccagtgac atcgtctcca gccacgtcgc ggtctggttc tcgatatcca gcccgaggcg
    23461 gtcacgcaaa tcgcgcagcg tgtcgctgag caccggacgg ttaaaggcga ggcaggccga
    23521 caggcaacag gcgcaggcga ggccgtgggc cgcatggaag cgtacgccca acggatgggc
    23581 caggccgtgc accacgccga gccgcgcgtg ggagagtgcc acacccgcca gaaaactggc
    23641 ctccagcatg gcggcggccc ggtcggcgtc gccgcgatac aaggggagca gattttgtgc
    23701 gatacgcgtg agggcgagtt cagagagtac ccgcgtccag ggggtggcgt gccgactggt
    23761 atacgattcg aacgcttgca caaaggcgtc cagacccgcc gcggccaacg tgtcgggcgg
    23821 gcagtcccgc agtagccgcg gatcgaggat caccagacgc ggcaggtagg agggatggcg
    23881 gatcgattgt ttgagtgtgc gttccggatc ggtcagcacg gcgactccag tcgcctccga
    23941 tccggtcccc gcagtagtgg gcgctgcgat cagcggcagg ggtgcgggcg ggatgagcga
    24001 ggcgtggctc atgtagaacg tcacgctttc tggcgcgtcg gccagtcccg cggccgcttt
    24061 ggcgaggtcc atgacgctgc cgccgccgac agccgccacc cagtcgggac gttccgtgcg
    24121 gatcgcggta cgtaaggctt ccaccgcatg gacggtcggc tcgccgcccg cgtggcacca
    24181 ggtgtgcacg gtcatgccag acggcggttg cgccaaaatt gcgtccagca ggccggagcg
    24241 ctggagtgaa gcgccgtgca cgagcaatcc gcgcgggccg aacccggcgg cttcctgcgt
    24301 gagatgcaat atcttgtccg gcccggcaag ggtccggttc ggatgcaaca gggtaggggg
    24361 aatcaggttc atggttttta agcgtaacgg aatcgcggcc gaatggggag cgaaaaaaga
    24421 tgcggatgaa aggtgaaatc aagattagat tgccggattc agagggaaac gatagcatac
    24481 ttttcttttc atgaaggttt gaggcggaac gtgcaacgat ccgccggggc agggcaaaag
    24541 catatgagca gattacgatt ttggaacgag cgggcggaaa ccctgtctcg ggacgagttg
    24601 acggctgtgc aactcgcggg actgaaatgg accgtcaacc acgtttggac caacaatgcc
    24661 ttttaccggg accggctcgc cgcgcatggc atcgagcccg gagatatccg ttcgctggat
    24721 gatctccggt ccctgccctt cctgaccaaa gacgacttcc gcgatcagta tccgctgggt
    24781 atgctctgca tgccgcaaga taccattcgg gagatgcaca tgagttcagg ttccaccggc
    24841 acgccggttg tgatggcgta caccgaggcc gatctgacgc aatgggcgga gtgcatggcg
    24901 cggtgtcagg tgatggctgg ccttcagcca ggagacgcga ttcagatcat gccgaccttc
    24961 ggtctgttca acggcggatt cggcttttat cacggtgcgc ggctcgcggg gatgttcgcc
    25021 gtgcccgcca gttccggcaa tacggcgcgc cagatcatgc tgctgcgcga cttcaaggtg
    25081 cgggccatgg gttcggtcgt ctcgtatgcg gcgcgtatca tggagatact ggatgcgaaa
    25141 cagatcgaac tgccccattt gaagatcggg attttcgggg cggagtcgtt ttccgacgag
    25201 atgcgcaacc gtgtggagtc acggctcgga atccgcgcat tcgacatcta tggaatgacc
    25261 gagacgggcg gggtggggac caccgggatg gattgcgagg cgcgctgcgg catccacgtc
    25321 tgggaggatc actacctcac ggaggtcgtg gaccctcaga ctggcgcgcc cgtgccggac
    25381 ggagcggtgg gcgaattggt gttcacctcg ctgacacgcg aagcggttcc ggtgatccgc
    25441 taccgtacgc gggatctatc gcgcatcgtc agccgggagc cctgcgcctg cggccgcacg
    25501 agtcttcgca tcgaccggat cacgggacgg tgtgacgaca tgctgatcat caaaggcgtc
    25561 aatttcttcc cgaagcaggt tgaacaggca ctgatggaaa tcccgggggt gttaccggaa
    25621 taccagatca ttctggagga gtgccacggg gtgacggatg tgcgcatcaa cgttgaggtg
    25681 gaggaggggg tcaccggtta tcaggtggtg aaacacctca agaagcggct cggattcagt
    25741 ccgaaaggtg atgtctttaa acccggcacg ctgccgcgcg gcgaaggcaa ggccaagcgc
    25801 gtgtttcgcg aggttcgcgg ttgagaaaac ggcgggccgc aggggttctg ccagtctgcc
    25861 gcacgggatg ggaagagaca aggaatcata tgaacgttca acgctcaaac gttgtcggct
    25921 aagagggaac atgcgctatc taacctacgt gggttgttat acggatgaga aaaaagaggg
    25981 catccacatc ttcgagacgg acagcgaaac cggcgcgttt tgtccggtcg gtctggtggg
    26041 cggcatcgag aacgccatcc acctgacgct gaatgccagc cggacccgcc tgtacgccgg
    26101 gctcggtatg ccggagtacg gggcgtccgc gcaaaacggc gctgttgcgg cgtatgaggt
    26161 gcagggtgat acgctccggc tgctcaacca ttgtccggta ggggtgacgc caccctgtta
    26221 tgtggcactc gatccttcgg aacaggcgtt ggcttttgca gagtacacga atgcggtggc
    26281 cggggtgttc gaattagctg aagacggccg gttcaccgat gcgccggccg tgacagtgca
    26341 gcacgagggc gaaggtccga gcaaaccccg gcaggacaag gcacacgcgc attgcgcgga
    26401 agtgacaccg gacggacggt atctctgcgt ggtagatctg ggccttgatc aagtgcgggt
    26461 atacgacttc cctgaacggc ggggcggatt gcgcgagcga tctacgctga ccatccgcag
    26521 cgtgggcgga gccggaccgc gccacatcct gttccatccc aacggacgtt tcgcgttcgt
    26581 cctgcacgag ttgaacaaca cgatctcttc gttccggtat acgggggagg agtttgtttt
    26641 ggtgcagacc gtcccgatgc ttccgccgga gttcaaggcg tacagtaagg cggcggcgat
    26701 caaactctcc gcggacggac gtcagttgtt cggttcgaac cgcgggcatg acagcatcgc
    26761 ggcgtatgat ctcgatcctg acagtgggcg catgtccttg ctggcgatct ccaaactggt
    26821 gggcgaattt ccccgtgatt tcgagtttct gcccggcgga aaattcgttt tactgggcca
    26881 tgagaattcg aacgagttgt tttcctatgc ctacgacgcg aagagcggcg tcttcacacc
    26941 ggcacacggc ccgttcagtc tccatcgtcc ggtgctagtc cgattcggag tccgccgtag
    27001 ctgacgcggg gcgcgtccct gttgttttaa cgcattgcgt ccggggggaa tgctcaaggg
    27061 gaaccatgaa acacatggaa cttcatgaaa ccagaccccc ccccccttgg cgtttctggc
    27121 gtcttggcgg tttgctcaaa tctgagccgg ttctgccgag tgtaacctat tacttcttac
    27181 ctattacctc ttacctatta cctcttacct attacctctt acctattacc tcttccttcc
    27241 gcgatcaggg tttagaggcg aaggagatca cgcagaagga gacgacgagc actgccgtgc
    27301 cgaatgcgag ataggccttg gtcttggcgc ttgttccttt ccattcgccc gtcatcacgc
    27361 cgatgatcgt ggagaagagg atc
//
LOCUS       DAOSRB010000013        26993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_1981_length_26993_cov_6.649863, whole genome shotgun sequence.
ACCESSION   DAOSRB010000013 DAOSRB010000000
VERSION     DAOSRB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 26993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26993
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1981_length_26993_cov_6.649863"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..2033
                     /locus_tag="PLZ74_01530"
     CDS             111..2033
                     /locus_tag="PLZ74_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97033.1"
                     /translation="MVSLYTVGIAGGVLGILLGFALPVCGSDLALRFRTPRENWTRPG
                     VELTFRTAHTNQAEVVRQLERAQALGFGSVLAAVEGPEEGVWPIVERLADTCHRLGLE
                     LGLHDFPFAGDGGKNRARCLVWTEEPSTAFGGTNALNRVVQENGALAVWAVPADRSGA
                     VHPHEMVDLTRAALPTTGVWRVFRFALREVTPPLTDGYAGNSFARHVNRTLSACQSRL
                     PQTYGTTLLWYQFNGPQRDDMVWPADLPERFLRNSGLNLMRFLPVLAGVPVGGEATAQ
                     YVRRQLRQSVREAWREGFARHADELVHEAGLEAGIAIDAVPVEPEEVPLSFRRPMFAV
                     SRDADAAGVNRRAAGVARVLRRRFVIGRLPLNDLAETPAATLLPFRWKPEAERLLVEG
                     ATRLLLVPSGDLPDEDAAFRSLREGCDTVRRCQVLLQHGEPVADFLVWTEGVPPVLEG
                     YACDYATPALMERATVSQGRIRFESDRTYNALVVPAGVMGREAARRRIGQIAARGVTV
                     WLMEEADGAAGTTAAETMPAGCRRLRPGAPDAPRPDLFWQSEQAGMRLSFLHRRTPER
                     EIYFLVNDTPEAGPVRCTFRDAGAGMPLRWDPVSGETDLVVQGASRTGDGRVTAPLFL
                     GPHDACFIVFERAHAN"
     gene            2118..5084
                     /locus_tag="PLZ74_01535"
     CDS             2118..5084
                     /locus_tag="PLZ74_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR97034.1"
                     /translation="MERFVQPENTARIQVPPTERLAAPAQDETQTVRVVPGEGTSHYE
                     EPGNDKLFDQYTIYSKIGNGGMGVVYLARDRRLGRFVAIKRLNHQAQSIPTLRQRFLH
                     EARAVAVLSHVHIVHIYALGEDEDGPFIVMEYIAGPDRPDVVSEMPPGGLLQPNPPLT
                     LDQYVSQHGQLSVDEAIELVLKIGRAVAYAHASGVIHRDLKPSNILMDKSGEPKIVDF
                     GLARLMSKEEAKLTVPGEKLLSLGYGAPEQESDASQSDERADVYGLGALLYFVLTGQN
                     PRYFREQDIPVSLRDVVVKALATDKEQRWESAAAFNTALYQIQSKTQIETPTVKTTWR
                     CKWCDAVNPLAIKFCAECGWDGSEACPECGADTFVGVQYCGTCGADARAYESVLHLLQ
                     KMRDSDTQQRFDRVIAHAGRVHGFEPAGPSGRAYLQEVTELRALAEKKMRRRDQLKEQ
                     IPIEIRAENFERAEEFIRQYRELTEDRQGFENELRQIPERILLRDLQRAQRALKQREW
                     DSAARLCGTLLREVAPDHPDVLRIRKAIRIHTAAANLRIACAITLGMMVLYLLSLPVA
                     VTLADRSFGTLARGFYRPARWVYERSLARVPLQAYARLWLGAPPEWNVYFATASEEPV
                     LPGEVQPTPQPDALAQKQREYARQLAELDARQEKFQRAWPTEYVRELDLLMERCRTAG
                     DFEGWDVAQTERRRFDETRQMNDAVVEGLSELNTLKTKYRQMLSEQRLQHSRKWVTLC
                     KKYVNDLTDMQRKFMQEGRMDLASAVNAEIRKARNAPPALAAEAVVAAAELAGMSETE
                     TAPMLLGGSDQHRVEEIAQLRSGFESELAAVEKDAVRKATAWPDQYVQGLTELMESFQ
                     RAGDYVGWESARDELGRFEADRAIQQRHFVLQPAGLVELQKKFFLLREENRRKRAEQL
                     VDTTEKYVKKLQDVQKKLTVGGQMETAAAVNAEIKRVRTRVDYIEAQNELSPPQGPPV
                     PPAVWPKTEPAAGGNG"
     gene            5081..5908
                     /locus_tag="PLZ74_01540"
     CDS             5081..5908
                     /locus_tag="PLZ74_01540"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOR97035.1"
                     /translation="MSASGLGGCGKGEAGMVDVWGRVRRQIPLTAGAWVMEMEAPEVA
                     AEARPGQFVHVRVPNLERSALRRPFSLYGAEGGVLRLLYKTVGRGTEQLSRVEPGERM
                     QVIGPLGNGFPLEPQGTPVLVAGGYGVAPLAFLASRLPVRGVVLVGGRSAADVLAIET
                     FTELGWRVRVATQDGSLGEQGLVTDLLDSELETMRGAEARVELFACGPDGMLRAVGDR
                     AIQTGCRAWLSLDKRMVCGVGACLACVQSLRRPDGSEWIGRVCHDGPIFEAREIIWS"
     gene            5905..6849
                     /locus_tag="PLZ74_01545"
     CDS             5905..6849
                     /locus_tag="PLZ74_01545"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOR97036.1"
                     /translation="MTGEVGAGMRDLDLRVNLGGIRMQNPVTVASGTFGYGREYAGLI
                     DVTRLGAITVKGIRLQPWPGNALPRHVEVPGGLVNAIGLQGPGVDGFVSEYMPFLRTT
                     GVPVIVNIWGTSVEEYAEVARRLTEVEGVAGLELNISCPNVKEGGNTFGTDPCAVSSL
                     VSAVRRATRLPLIPKLAPNVPDIRVFVRAAQEAGADALAMINTLPAMVIDIETRKPVL
                     ANRVGGLSGPAIHPVAVKLVWEAAQVAAVPIVAMGGIMGAEQAIEFLIAGASAVAVGT
                     ANFIDPSTPLRVLDGLESYLRRHGMRSVGELTGSLCCG"
     gene            6868..8016
                     /locus_tag="PLZ74_01550"
     CDS             6868..8016
                     /locus_tag="PLZ74_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOR97037.1"
                     /translation="MRNEWYYAVEGERFGPVTFTELRRLAQTGRISEYDLVWHPEFGP
                     EWRNAGQVREMFEVPYREAAAPPISGMGEVTLGRPHALRAASQAFARMTALLFRPFDP
                     VRWFSIGFCAWLAYLGTQSTVNLQQLGETSAGGFKQQVDRMIDVLQLLPEQPVKLAWT
                     GFAILLGLLFALWMCWVRSRGDFMFLHRWYKPDTPVHACWMASRSSGWALFGWRVLFF
                     LVAAALFVLHAAIAYTHVLKPYLDAGRIWQPALLPPVTGCVTSLALLLIAVRLVAYLT
                     QAFVVPLMYWSGCGVASAWRMVLALVNRHPFALTGYLLCGMACWSAAMFAVLVFGLVT
                     CCIGFIPLVLPYLNAVLLLPVTFFFRGYAVCFLSQWRPDLVPAAEGDM"
     gene            8016..9263
                     /locus_tag="PLZ74_01555"
     CDS             8016..9263
                     /locus_tag="PLZ74_01555"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HOR97038.1"
                     /translation="MIPHELVRALPKSDVHAHLDGSLRIATLIELAREQRVPLPSYEE
                     EGLLELVFKPVYRDLADYLRGFEYLCAVLRRRDALERAAYEMAEDAIAQGVRYLEVRF
                     APQRLVETGEACVQAFKAVNDGLARAAREHNASARVHAQEDIPFEWAILCCAMRNFER
                     GMGAYYDRLLDVLPGMARNELVSIASLEAVRAAVKARDREGVPVTGFDLAGEEAGYRA
                     GHHVTAYEEAHRHFIRKTVHAGEAYGPESIYEAIARCHAERIGHGTFLFAADKILDPS
                     ISDPQAFTDSLADYIATMRVTIEVCPTSNLQTIPELGGDLRKHPVRRMLACGMSVAVG
                     TDNTLVSRTNINTELLRVAEVCELDRRAFQRLVLAGFKAAFYPGRYEVKRRFVRRAAE
                     RIEQLLGTVPKHAAGKKEAACGS"
     gene            9251..10882
                     /locus_tag="PLZ74_01560"
     CDS             9251..10882
                     /locus_tag="PLZ74_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOR97039.1"
                     /translation="MRFLTVDQMRAADRDAVTEAGIPERALMRRAGEALACAVARVAA
                     RRRTRAAVLVAGHGNNGGDACVAARALREAGFLVRVLLTAPVARFRGVAREAWEAMDA
                     AGVPWSEPEAAESACGGWTEDNADAVAAPSAWGGVVVDGVLGTGCAGAPSGAAARAIG
                     WINRQRAHAMIVAADIPSGVHGDSGEAAGVAVHADVTVTFARPKRGLLNPCGAERFGH
                     VIVADIGLPDALCDRGTDDAPCECIALPELARFFPRRQWSAHKGTCGHVAVIGGAAGM
                     PGAPVLAALGAVRSGAGLVSLAVPPQSAAVAATWVPEAMCHTLDVPRGVISPHALAAG
                     FSGLDACKVVIAGPGLTRHEEAGRTVAQLLETGPDRLLLDADGLNALADLFHAGWRPR
                     ARQDLLLTPHPGEAARLLGLSVAAVEADRVAAVRLLAERYRAVVVLKGAGTLVCAPDG
                     RPWLNRTGNPGMASGGMGDLLAGMVAALWAQGLDPVQAACTAVWAHGAAGDEAAWEEG
                     RTALSATRLAAALGSVYGELERLAAGSEEGGGNVG"
     gene            10872..11618
                     /locus_tag="PLZ74_01565"
     CDS             10872..11618
                     /locus_tag="PLZ74_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97040.1"
                     /translation="MSDDLTDRQVLTRLAWLFALFAVISLAAGASRMQPPGWQLRTFG
                     DVLRLREALSMFVFAPALGLVFWLIGRTLSRGRTVVAADLLMVLAVYGVACGMGIHDP
                     TNRMISAYSGSGALTPEVRRSLFYLDEVLGHWVFWAGFVLATWVLGLRQALAPLKERM
                     QWPWRVGLGVVAAAFLWVMVTNLWDEYPQTRADLGVILLAALGPLAAHVAFGRNAGVL
                     RMPLHFVIYPTYFGSVAVTLINWLIRYGRV"
     gene            11660..12550
                     /locus_tag="PLZ74_01570"
     CDS             11660..12550
                     /locus_tag="PLZ74_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOR97041.1"
                     /translation="MAIRSMTGFGRGEAEENGRGVTVELSAVNRKQFDCSLSLPRELS
                     SLDTRIQTLIRTRVSRGYVKGVVTVATSVGEAGGNAAGWDPEVIGKHLTALRDLAKRL
                     GLADDLTASSLLCLPDLLRGAAPLEPEKMWPLVERAGTLALESLDTMRQREGAALEQD
                     LRKRLTVLEKTAEQIAARAPMVPVTYQTLLTKRLGELLGEEGTAVDAALVAREVAVFA
                     DRCDVSEELTRLGSHFAQVAKTLDSGGTCGRTLDFLCQELFREINTTGSKANDAEISR
                     HVIAFKAALEAMREQVQNIE"
     gene            complement(12620..12940)
                     /locus_tag="PLZ74_01575"
     CDS             complement(12620..12940)
                     /locus_tag="PLZ74_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HOR97042.1"
                     /translation="MKKSLVLSLLCASVLMTTGCLSPMKGSIMAVFTVDHVASDPVVD
                     NTVRPAKRGQAKATGIICFATGDASIGAAMRNGGITKIHHVDYDVKNILFLYNEVTTI
                     VYGE"
     gene            complement(13113..14405)
                     /locus_tag="PLZ74_01580"
     CDS             complement(13113..14405)
                     /locus_tag="PLZ74_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOR97043.1"
                     /translation="MFPSQDLDILTSMVLTTEIPRVVICFSAAIKTMRELMQGVLEYA
                     KANGPWALHILAGRNLEQKLPDLVEWGCNGLIGNLSDEQFVDMKIPADIPLILNGRAS
                     NEDDRPVDYPSNTIGLIECDNVPIGRAAAEFFLSKGFTAFAYVGDVNRPQWSIRRGEV
                     FARCVAARGFECHHYTLPPISVARNAVREQRTLARWLDALPKPIALFAANDTRARQVV
                     NLCLENGIAVPQEISILSCDNDELLCESCVPSLSSIKMNPFQAGYEAASLLDRAMHEG
                     FRRRHRPIRVTYTLDSIVSRLSTADLRVKDPLVERALTCIRLNSAADFSVGDLAEKLK
                     VSRRLLEMRFRKVLDSTVHNAIMSARLERVCEVLKSTRLPIQDIADRLHFSSASHLGI
                     IFKRQFGTTPSRFRRELSGGGRNDFRLMAGDSTTVSRI"
     gene            14667..15857
                     /locus_tag="PLZ74_01585"
     CDS             14667..15857
                     /locus_tag="PLZ74_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOR97044.1"
                     /translation="MAQSSQTHLVTIGYELTGADAIVTLDITTNGVSIGAANIRGLVG
                     DVNKRVSPGQRAIQWNPNKSWKNNVVSDGSAKAVVKIWSLSEPPLYMTASLDTANCVR
                     YYDCADAIEGGITNDMYKRTHLLLRKVPATGVTWRMGENSRNSGEYTGANETPHLVAF
                     TNDYYISVYQFTQGQIAELRGLGWVGGCNYNKASHDDWWSHPMESINYDYLRGATKGA
                     QWPTTDEFDADSCFAVLQNRTGMRFDLPTDAQWEFAARAGNQLASSTVAVDTYTSGWE
                     IMNPYAWTGWNSGDNGGSENKRTHAVGTKLPNNWGIYDMQGNVWEMCRDWFEQVCAFP
                     ADGSPVVNPLGPISGSTRVMRPGGYDNSTHSVHRYTFRNHIDPSGSASGSVGFRVVCE
                     ITGE"
     gene            15870..16142
                     /locus_tag="PLZ74_01590"
     CDS             15870..16142
                     /locus_tag="PLZ74_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97045.1"
                     /translation="MENVKEKMVAVAVFMAMAAVAEPAWTFTIEDHPAEVTASATTTD
                     AVAVASVTSQEAPSGAGGLTAFVSAFFESLGINLRSDPFGTLFLFK"
     gene            16162..17436
                     /locus_tag="PLZ74_01595"
     CDS             16162..17436
                     /locus_tag="PLZ74_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOR97046.1"
                     /translation="MERAKWKMAAAVAGRRVLPLLAFAAFTALAADPVVSGVTMTQDA
                     TTKNVTIGYTLSGADAIVTLDVTTNGVSIGGENLTKVWGDVNRKVVTGTRSIMWRPVM
                     GTADMKPVVTAWRTDTPPDYMVIRMDRPQGATNVVPLYYANTNSIPGGFNNARYKTTH
                     MIFRKCPAAEVVWRMGTPSSDEFFEVDQVAHLVKLTHDFYIGVYPCTQGQFTALAGVG
                     WRGRCGYTYSTNIDWAVHPMESINYDDLRGKTAGAQWPTSGDVDAGSFFGVLRAYTGM
                     NFDLPTEAQWEYACRAGTGTTGNIGVSYGTCWDEVSWINYNADGHTHEVGLKRPNAWN
                     IYDMHGQVMEICRDWYGPYEFVDDETPVVDPEGAPSGEYRVARPAAFTNNHTPNHSGF
                     RNKMKPSEGDMGGWHWNGSTYFAGFRVALEIE"
     gene            17573..19885
                     /locus_tag="PLZ74_01600"
     CDS             17573..19885
                     /locus_tag="PLZ74_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HOR97047.1"
                     /translation="MARVTPEWAGKVVFEDVARKAGEKEFCEVRSLPDGRIAVKGNTP
                     NAQCAGYGKYLRKVAKVPYFWEGGGTAAVAGRHAPPRMDGVIRAEAPYQWRAVFNFCT
                     HSYSLAFADKARWERELDLLALRGVNLPLACTGVECVWYETLMSLGWSDEEARSFLVG
                     PAFMAWQWMTNIESYAGPLPKSWIENRLALARFILARERELGMTPIQQGFTGNVPRDT
                     VKKFPSAKIKFQPPWCGFPGSAQLDPLDPLFPKFARAFYAAEKKLFGLHGYYAVDPFH
                     ESQPPQAGDDYLRAVGETIGRELRAADPAAKACMQSWSIRVPILKSFPKDGIIVLDIS
                     GEKGRHTKGFWGYPFVTGAIINFGGRTASGGDIRWLAENHFAETYRKFPNCVGMGYFP
                     EGIHTSPLYWEQALDLLWRDDNPEPLAWTTELLEARYGVPSATAKSLAEGLLATYYGR
                     VHLASAVYPAKPTFLLKRCDPNVGFRSGYDHRRLLRTWREMITAAKEFGVRPGMRFDL
                     VDAGHRLLSDLSYAQFVDVVEMFLKGDVKEYDCAVAAFLETGADEDWLRKHEPLSSLK
                     TYLDLAAAQGKTDEERNLYTFNQAVQVTYWGPFGVPTVAGDPIQEYAWKEWGGLLSGY
                     YLPRWKMFLEHARKVLEGKAELADIVPPTDIWQRPKHRGNAFYSKMADFEEGWIRDPK
                     RMTGDAPTDDVVSACEKVLAKQAAAFEYQFENGKEKINGFEAKIAEFEKEIQRRIKAG
                     RVRADVTGDGKENDTADLMRQVGDQMKEEK"
     gene            19882..22002
                     /locus_tag="PLZ74_01605"
     CDS             19882..22002
                     /locus_tag="PLZ74_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97048.1"
                     /translation="MKSSKIMNSCTFVVLVVSPFACSLSHLNAAEFRVLPGDDVKAVV
                     AQADAVRRQKPTEPIDIVFAKGDHEITVPLFLHRTKNPIGDGQAATMFRGEEGARVLG
                     GTRVGPWRKCAFNGRTDTWCADVPKDVERIYTFHADIEIRTECRYPNFDPEHPETGGW
                     LFIPGKMFSMYSLREGERKDRIPMRAEDWKTWAKPSEGRVNIFPRYNWWNRIPPIAAA
                     DEATHTLHLAGDTGEFPRPADRFYLIGLREELDAPNEWYFDADAHRIWFIPPAGMDPN
                     KVRTAITTTTQPVFNFNDVGNVHFRDLEICEGGGGIKLYDGSRNCTVTHCTIHDTGWF
                     AGCGIQTWGSGIVIRECRVWNTGNSGVQVHSLRDASPAAYAPGHVDGNEVSFCHIHHT
                     GRINGCGECICGAGQGTRIVNNLLHHAPRGAISFGGRMNDISRNRIHDTNFQSEDTGA
                     IYTGGYVNNAGTKINDNWISDSYGFSHGGTGVWEHRKADAFGIYLDDSSGGAEVVGNL
                     VERCTQGAIFFHSARFNTVSNNFFISNGGFGKYRLQITMNGFTTNAPPYLKTAAQDQY
                     DHMMKGGAAWTNYPSLRLPPTAFEHSEGMLMIGNQIVNNVVYYPDQPESFYTAPGQFA
                     PKATLFDRNVIWCGKGNRVRYGRNFKEIPAEKWAEMGQDLHSVVADPLFVDPARGDYS
                     FKPDSPALKLGIRPFKVSHYGPNY"
     gene            22096..24210
                     /locus_tag="PLZ74_01610"
     CDS             22096..24210
                     /locus_tag="PLZ74_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97049.1"
                     /translation="MGRTVRPARPWVRGTRVPAVVLLVLSFTFFIFNPSFAQGDEGHA
                     ATVTWRIHENQWRTEEEFKKLTKFFDEHPVTGKIAFFTCPSHVPPKFEHFLPCLPQLK
                     KAIAHFKARGHRTGINHLCTIGQTDEDLDRAIVIEGAQYLVNWKGETAKSARCPLDPV
                     WREKYVKPIYTVLAETGPDFIWTDDDIGRLSGHGVPGPGCFCPICMKRLHDRLGYSGD
                     VKGLNAFFTDAEKGEARRRAMMTLNREVYADLLKYIREVVHAVDPKIVIGVMDSITAY
                     TGNNYEEKFRALAPTGTEEVFFRPGGGYYNDIGGLGGVLSKANEMGYESAYLPDGVAV
                     NESEIETFQYQLLHKANFSLKTESLAYMAAGTRGTAWNVFPFADNETYEAVAPRIESC
                     EKVRVEADAIVAACGRAKPRGVWNGRGRDYFVGNKNGGRTGEWMQEEWWEKGFWQSEL
                     QMTGLPAAYREEECEVCAPNAQAVWSWTKEQLERRLAGGLYLPLDAAQAIIDRGYGED
                     LGFKIGKAVSKDARERFVDHPLNAGVAGFRRDMRQSFWGGDVIELEPMEGAQILADAV
                     DIPGRVVAKCVSGVYENRRGGRIFMTGTMAWDRLSFSPVVRERKNVMRWLSRETLTGY
                     IDSYHKAAMWVRGDRAIAIYNMSFDAAEGMEVLLKGEAWSKGIRLSGGDGTVIRGVRE
                     GAYTRYRLPKLAAWSVTAWARM"
     gene            24383..26956
                     /locus_tag="PLZ74_01615"
     CDS             24383..26956
                     /locus_tag="PLZ74_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97050.1"
                     /translation="MGGIVYDIDFGSATNQVARDGVGKINGVLPDDFAENYTGWCDGI
                     ATTEVKSEEGIGKYLRLTTSAQTKGGTQFVVPKELKPGCYRFTVESRTLGAVTYRCSF
                     RMGGSPYSDCLSRHVMSENWSVQSWCFSVPKDWKQLPMLFVNTRPGVVDLKRMKVETA
                     TIAEMAQEAVRRPSKDVTEYIRHNRFPLGLPTGWCFRRDCEGTVYGADETEPAPDGVP
                     VLKVSGNRMWTFYSEAFQTAFPGGEMEVSFKYRSDCRVRNFVNDDHWRGEMFGESWLG
                     PTKEWKEHRFRFRGPELAEAFTIGWGTGVEAGTMWLDDVRVRPMDGTGVRDTRPCLQM
                     AVVEGDAGEGTRVQFLDEKPLVKWCVVDVPTGSVMKVEAADLYGEEVTIAEIPLKGGK
                     LEKGTLEISAFKDCPMGQFRICATIEKDGKVVSEREEIVLTRVVRPVMWGKDAPNSPF
                     ASHFNPWRPVVKMMKACGVNWVRFHDAAEDLSNWFYLERKKGEWTFRDAAIDEYRRAN
                     VKIFAQLGMSPEWANHSADLVKENPKWTPGYFGRYLRPTNSVDWVNYVTTFAKHYRGV
                     IDEYFVWNEPGGHSWQFNDDAKWFAPGRAGQDFAVLQRQTYDALKAVDPKLKLFGFNA
                     GGPKEWVEDVMDGGGFDCCDGIDWHYYSVPSLAHRETANVMEKYSLPSIRAKYPDFGG
                     KPLYMSEGQGTNDGGSKLRCHNTGLYHATVPWTPDTMQDYLELSDNTARFLVQLLADG
                     FKKVFLYTCHGYVHPARRSEFSVLIGKDGYPVPSFVAHAALAQTIEGKTFVNKEDFGE
                     KGCVFTFRAKDGKNARVYSDLSEKEKLALAKKTCLHDVFGNPVTEKTAMPDTLVYEPG
                     K"
BASE COUNT         5002 a         7199 c         9724 g         5068 t
ORIGIN      
        1 accccgaaac cttgaaacca aagagagttg aaggttcaac tgctcttttt aggttcaatc
       61 tcaccgtaat ccggttggtc ggggacatcc gggtatggta tgctcaattt atggtttcac
      121 tttacacagt ggggatcgcg ggaggcgtgc tggggattct gctcgggttc gcgttgccgg
      181 tgtgcggttc ggatctggcc ttgcggttcc gcacgccgcg agagaattgg acgcggcccg
      241 gggtggagct gaccttccgc accgcgcaca cgaatcaagc ggaggtcgtc cggcaactcg
      301 aacgtgcgca ggcgctcggg ttcggcagtg tgctggcggc tgtggagggg ccggaggagg
      361 gggtgtggcc gatcgtcgaa aggcttgcgg acacctgcca caggctaggt ctcgaattgg
      421 ggttgcacga ttttccgttc gccggggacg gcggcaagaa ccgtgcgcgg tgcctggtct
      481 ggaccgaaga gccgtccacg gcattcggag gaacgaatgc gctgaatcgc gtcgtacagg
      541 agaacggcgc gttggcggtc tgggctgtgc ctgcggatcg gagcggagcg gtgcatccgc
      601 acgagatggt ggatctgacg cgcgccgcgc ttccgacaac cggggtgtgg cgtgtcttcc
      661 gtttcgcgtt gcgggaagtg acgccgcccc tcaccgacgg gtatgcgggc aacagttttg
      721 cccgccatgt gaatcggacg ctttcagcct gccagagccg gttgccgcag acgtacggga
      781 ccacgctgct ctggtaccag ttcaacgggc cgcaacgcga tgacatggtg tggcccgctg
      841 atttgccgga gcgtttcctg cggaatagcg gcctgaacct catgcgtttc ctgcccgtgc
      901 tggcaggtgt gccggtgggc ggggaggcga ccgcgcaata tgtccggcgg caactcaggc
      961 agagcgtcag ggaggcgtgg cgcgaagggt tcgcccggca cgccgatgag ttggtgcatg
     1021 aggcgggact cgaggcgggg atcgcgatcg acgctgtgcc ggtcgagccg gaggaggtgc
     1081 cattgagctt ccgacgcccg atgtttgcgg tttcgcggga cgcggatgcg gctggcgtga
     1141 accgccgtgc ggcaggggtg gcgcgcgtgc tcaggcggcg tttcgtgatc ggacgtctgc
     1201 cgctgaacga tctggcggaa acgcctgccg caaccctgct gcccttccga tggaaaccgg
     1261 aggcggagcg ccttctggtg gaaggggcca cacggttgtt gctggtgcct tcaggggatt
     1321 tgcctgatga agacgccgcg ttccgctcgc tgcgggaagg atgcgacacg gtgcggcggt
     1381 gccaggtgtt gttgcagcac ggcgagccgg ttgccgattt tctggtctgg acggaaggtg
     1441 tcccgccggt cttggaggga tatgcctgcg actacgccac accggctttg atggaacggg
     1501 ccacggtcag tcagggccgg atccgctttg aatcggatcg cacgtataac gcgctggtgg
     1561 tcccggccgg cgtgatgggc cgggaggcgg cgcgacggcg gatcgggcag atcgcggcgc
     1621 gtggggtgac cgtgtggctg atggaagagg ccgacggcgc ggcggggaca actgcggcgg
     1681 agacgatgcc cgcagggtgc aggcgactgc ggcccggtgc gccggacgcg ccgcggcccg
     1741 acctgttctg gcaaagcgaa caagccggca tgcgcctctc tttcctgcac cgccgaacgc
     1801 cggaacggga aatctacttt ttggtgaatg acacgccgga ggccggtccg gtacgctgca
     1861 ccttccgtga cgcgggggcg ggaatgccgt tgcgctggga cccggtgagc ggcgagaccg
     1921 atctggtggt gcagggcgcg tcaaggacgg gtgacggacg ggtgacggcg ccgttgtttc
     1981 tgggccctca tgacgcctgc tttatcgttt tcgagcgggc acacgcgaat tgacgcatgg
     2041 gaccgcaagg ccgtatactt ataatccgat ccgccgatgt gggctgtttt tgaccggccc
     2101 ccggaacgcg gatacgattg gagagatttg tgcagccgga aaacacagcc cgaattcagg
     2161 ttccgccgac cgagcgtctg gccgcgcccg cgcaagacga gacgcagacg gtacgcgttg
     2221 tgcccggtga gggaacatcc cactatgagg agcccggcaa cgacaagctt tttgaccaat
     2281 acacgatcta cagcaaaatc ggcaacggcg gcatgggtgt cgtgtatctg gcgcgggacc
     2341 gccggctggg gcgcttcgtt gcaatcaaac ggctgaacca tcaggcccag tccatcccga
     2401 cgctgcgcca gcgatttctg cacgaggccc gcgcggtggc ggtgttgagc cacgtccata
     2461 tcgtgcacat ctacgcgctg ggcgaggatg aggacggccc gttcatcgtg atggaataca
     2521 tcgccggtcc ggatcggccg gacgtggtga gtgagatgcc tccgggcgga ctgctgcaac
     2581 ccaatccgcc gctgaccctt gaccagtatg tgtcgcagca cgggcagttg tcggttgacg
     2641 aggcgatcga actggtgctg aaaatcggac gcgccgtggc gtacgcgcat gccagcggcg
     2701 tgattcaccg cgacctcaag cccagcaaca tcctcatgga caagagcggc gagcccaaga
     2761 tcgttgattt cgggttggcg cgcctcatga gcaaagaaga ggccaaactc acggtccccg
     2821 gcgagaagct gctgtcgtta ggttacggcg cgcctgaaca ggagtccgac gcgagccaat
     2881 cggacgagcg tgccgacgtg tacgggttgg gcgcgttgct ctacttcgtc ctgaccggtc
     2941 agaacccgcg ctactttcgc gaacaggaca ttcccgtgtc gctgcgggat gtggtggtca
     3001 aagcactcgc caccgataag gaacagcggt gggaatcggc cgcggccttc aatacggcgc
     3061 tgtaccagat acagtccaag acgcagatcg agacgcctac ggtcaaaacc acctggcgct
     3121 gcaaatggtg cgacgccgtc aatccgctgg cgatcaaatt ctgcgccgag tgcgggtggg
     3181 acgggtcgga agcttgtccc gagtgtggag ccgacacgtt tgtgggcgtc caatattgcg
     3241 gcacatgcgg tgcggacgcg cgggcgtacg aatcggtgct ccatctgctg cagaaaatgc
     3301 gcgattcgga tacgcagcag cgtttcgatc gtgtcatcgc ccatgccgga cgtgtgcatg
     3361 ggttcgaacc ggctggaccc tccggccgcg cctatctgca ggaagtgacc gaactgcgtg
     3421 ctctggcaga gaaaaaaatg cgtcggcgcg atcaattgaa agagcagatc ccgatcgaga
     3481 tccgcgccga gaattttgag cgcgccgaag aattcatccg gcagtaccgc gagttgacag
     3541 aggaccgcca ggggttcgaa aacgaactgc ggcagatacc cgagcgcatt ctgctgcgtg
     3601 atctacagcg cgcgcaacgt gcgctgaagc agcgcgagtg ggattccgcg gcgcgtctct
     3661 gcgggacgtt gctgcgcgag gtcgcgcccg atcatccgga cgtgctgcgg attcggaagg
     3721 cgatccggat acataccgca gcggccaatc tgcggatcgc ctgcgcgatc accctcggga
     3781 tgatggtgtt gtatctgctg agcctgccgg tggccgtgac gctggccgac cggtccttcg
     3841 gcacgctggc gcgcggtttc tatcggccgg cccggtgggt gtatgagcgc agtctggcga
     3901 gggtgccgtt acaggcctac gcccgactct ggctcggtgc cccgccggaa tggaacgtgt
     3961 atttcgcaac cgcttcggag gagcccgtcc ttcccgggga ggtccagccc acgccccagc
     4021 cggacgcgct ggcgcagaag cagcgcgagt acgcgcgcca actggcggaa ctcgatgccc
     4081 ggcaggagaa gttccagcgg gcgtggccaa ccgaatacgt gcgcgagttg gatctcttga
     4141 tggaacggtg ccgcacggcg ggagacttcg aggggtggga tgtggcgcaa accgaacgca
     4201 ggcggttcga tgagacacgg cagatgaacg acgccgttgt cgaggggttg tccgaactga
     4261 acacgctgaa aaccaagtac cgccagatgc tttcggaaca gcggttgcag cacagccgga
     4321 aatgggtgac gctctgcaaa aaatatgtca acgacctgac ggatatgcag cggaagttta
     4381 tgcaggaagg gcgcatggat ctcgcctctg cggtgaacgc cgaaatccgc aaggcgcgca
     4441 acgcgcctcc ggctctggct gcggaagcgg tggtggcggc ggccgagttg gcgggcatgt
     4501 ccgagaccga aacggctccg atgctgctgg gcggttcgga ccagcaccgc gtggaggaga
     4561 tcgcgcagct ccggagcggg ttcgagagtg agctggcggc ggtggaaaag gatgccgtgc
     4621 gcaaagccac cgcctggccc gatcaatatg tgcaggggct gacggaactc atggaatcgt
     4681 tccagcgagc gggcgattat gtggggtggg agagcgcgcg cgacgagctc gggcgttttg
     4741 aggccgaccg cgcgatccag cagcgccatt ttgtgttgca gcctgcggga ctggtcgaac
     4801 tgcagaagaa atttttcctg ctgcgcgagg agaatcggcg caaacgggcc gagcaacttg
     4861 tggacaccac ggaaaagtat gtgaaaaaac ttcaggatgt gcagaagaaa ctcacggtgg
     4921 gcggtcagat ggagacggcg gcggcggtga atgccgagat caaacgggtc cgcacgcgcg
     4981 tggattacat cgaggcgcag aacgagttgt cgccgccgca ggggccgccc gtgcccccgg
     5041 ctgtgtggcc gaagaccgag cccgccgcag gcggaaacgg atgagcgcgt ccggcctcgg
     5101 cggatgcggg aaaggggaag ccggtatggt ggatgtgtgg ggtcgggtgc gccgtcagat
     5161 tccgttgacg gccggagcct gggtgatgga aatggaggca cccgaggtgg cggccgaggc
     5221 gcggcccgga cagtttgtgc atgtgcgcgt gccgaatctg gagcgttcgg cgttacgccg
     5281 tcccttcagc ctgtacggcg cggagggggg tgtgttgcgg ttgctttaca aaacggtggg
     5341 acgcggcacg gaacagttga gccgcgtcga accgggggag cggatgcagg tcatcggtcc
     5401 gttgggtaat gggttcccgc tggagccgca gggtacgccg gtgctggtgg ccggggggta
     5461 cggtgtggca ccgctcgcct ttctggcgag ccggttgccg gtccggggtg tggtgctcgt
     5521 cggcgggcgg agcgcggcgg atgtgttggc catcgagacc ttcacggagt tggggtggcg
     5581 ggtgcgggtg gccacgcagg acggttcact cggcgagcag ggtctggtga cggacctgtt
     5641 ggacagtgag ttggagacga tgcgcggtgc ggaggcgcgg gtggagctgt ttgcatgcgg
     5701 accggacggt atgctgcgcg cggtgggtga tcgcgcgatt cagaccggat gccgcgcctg
     5761 gctctcgctg gacaagcgca tggtctgcgg ggtgggggcc tgtctggcat gcgtgcagtc
     5821 actgcggcgg cctgacggtt cggagtggat cggacgcgtc tgtcatgacg ggccgatctt
     5881 tgaggcgcgg gagatcatct ggtcatgacc ggggaagttg gggcaggaat gcgggatttg
     5941 gatttgcgtg tcaatctcgg cgggatccgg atgcaaaatc cggtgacagt ggcttccggc
     6001 acgttcggat acggcaggga gtatgccggg ctgatcgatg tgacccggct cggcgcgatc
     6061 accgtcaaag ggatccggct ccagccttgg ccgggcaatg cgttgccgcg ccatgtggag
     6121 gtgccgggcg gtctggtgaa cgcgatcgga ttgcaggggc ccggcgtgga cgggttcgtg
     6181 agcgagtata tgccgtttct gcgcacgacc ggcgtgccgg tgatcgtcaa catctggggc
     6241 acgagcgtgg aggagtacgc ggaggtcgcc cgccgtctga ccgaggtcga gggcgtcgcc
     6301 ggacttgaac tcaacatctc ctgcccgaat gtgaaggagg gcgggaatac gttcggcacg
     6361 gatccgtgcg cggtatcctc tctggtgagc gcggtgcgcc gggcgacccg gctgcccctg
     6421 attccgaaac tggcgccgaa cgtgccggac atccgggtgt tcgtgcgcgc ggcgcaggag
     6481 gcgggggccg atgcgctggc gatgatcaac accctgcccg ccatggtgat tgatattgaa
     6541 acgcgcaagc cggtgcttgc caaccgggtg ggcggccttt ccggcccggc catccatccg
     6601 gtcgcagtca aactggtgtg ggaggccgcg caggtcgccg cagtgccgat cgtggccatg
     6661 ggcggcatca tgggtgcgga gcaggcgata gagtttctga tcgcgggcgc gagcgcggtg
     6721 gcggtcggca ccgctaattt catcgacccc tcgaccccgt tgcgcgtgct cgacggattg
     6781 gagtcctacc tccgtcggca cgggatgcgt tcggtcggcg agttgaccgg atcgttgtgt
     6841 tgcgggtgat gcggaaagga tgagccgatg cggaacgagt ggtattacgc ggtggagggt
     6901 gagcggttcg gtccggtgac cttcacggag ttgcgcaggc tggcgcaaac cgggaggatc
     6961 tcggagtacg atctggtgtg gcaccccgaa ttcggcccgg aatggcgtaa tgcgggccag
     7021 gtgcgcgaaa tgttcgaggt gccgtacagg gaagcggcgg caccgccgat atccggcatg
     7081 ggggaggtga cgttgggtcg gccccacgcg ttgcgggccg catcgcaggc cttcgcgcgc
     7141 atgacggctc tgcttttccg gccgttcgat cccgtgcgct ggttcagcat cggtttctgc
     7201 gcgtggctgg cttatctggg cacgcagtcc acggtcaatc tgcagcagtt gggcgagacc
     7261 tcggccggag gattcaagca acaggtcgac cggatgattg acgtcctgca gctgttgccg
     7321 gaacagcccg tgaaactcgc gtggacaggg ttcgccattc tgctcggcct gctgttcgcg
     7381 ctttggatgt gctgggtgcg gtcgcgcggg gacttcatgt ttctgcaccg ctggtacaag
     7441 ccggatacgc cggtgcacgc gtgttggatg gcttcgcggt ctagcggatg ggcgttgttc
     7501 ggttggcgcg tcctgttctt tctggttgcg gcggcgctgt tcgtcctgca tgcggcgatc
     7561 gcgtacactc atgtgctcaa gccgtatctc gacgcgggca gaatctggca gcccgcgctg
     7621 ttgccgcccg taaccggatg cgtgaccagt ctggccctgc ttctgatagc ggtccggctc
     7681 gtggcctatc tgacccaagc tttcgtggtg ccgctgatgt attggagcgg ctgtggtgtg
     7741 gctagcgcct ggcgcatggt cctcgcgctc gtcaaccgtc acccgttcgc gctgaccggc
     7801 tatctgctct gcggtatggc ctgctggagt gccgccatgt tcgcggttct cgttttcggg
     7861 ttggtgacct gttgcatcgg cttcattccg ttggtcctgc cctatctcaa cgcggtgctg
     7921 ctgctgcccg tcaccttttt cttccggggg tacgccgtct gtttcctgag tcagtggcgc
     7981 cccgatctgg ttcctgccgc agagggggat atgtgatgat tccgcacgaa ctggtccgtg
     8041 ctttgccaaa gagcgatgtg catgcgcatc tggatggctc cctgcgcatc gcgacgctga
     8101 tcgaactggc ccgcgagcaa cgggtgccgt tgccctcgta tgaggaggag ggactgctcg
     8161 aattggtgtt caaaccggtt taccgcgatc tggccgatta tctgcggggg tttgagtacc
     8221 tctgtgcggt gttgcgccgc cgtgacgcgc tcgagcgcgc ggcctacgag atggcggagg
     8281 atgccatcgc ccaaggcgtg cgatatctcg aagtccggtt tgcgccgcag cggctggtgg
     8341 aaacgggcga ggcgtgtgtg caagcgttca aggcggtgaa tgacgggctg gcccgggctg
     8401 cgcgggagca caacgcgtca gcgcgcgtgc atgcgcagga ggacatcccg ttcgaatggg
     8461 ccatcctctg ttgcgccatg cgcaatttcg agcgcggtat gggtgcctat tatgaccgac
     8521 tgttggatgt gttgccgggc atggcgcgca acgaactggt ttccatcgcc tcgctggagg
     8581 cggtgcgcgc ggcggtgaag gcgcgcgacc gcgagggggt gccggtgacc ggtttcgatc
     8641 tggccggcga agaggcggga taccgcgccg gccatcatgt gacggcatat gaggaggcgc
     8701 accggcactt catccgcaag accgtgcacg cgggcgaggc gtacggcccc gagtcgatct
     8761 atgaggcgat cgcccggtgt cacgccgaac ggatcgggca cggcaccttt ctgttcgcgg
     8821 cggacaagat cctcgatccg tcgatttccg accctcaggc cttcaccgat tcgttggccg
     8881 actatatcgc gaccatgcgt gtgacgattg aagtttgccc gaccagtaat cttcaaacca
     8941 ttcccgaatt gggcggcgac ctgcgcaagc atccggtgcg gcgcatgctc gcctgcggca
     9001 tgtcggtggc agtgggtacg gacaacacgc tggtttcccg tacgaacatc aacaccgaac
     9061 tgctgcgggt ggcagaggtg tgcgagcttg accggcgcgc cttccaacgc ttggtgctgg
     9121 ctggtttcaa ggccgcgttc tatcccggtc ggtatgaggt gaagcggcgg tttgtgcggc
     9181 gggccgcgga acggatcgag cagctcctgg gtaccgtgcc gaagcacgct gcggggaaaa
     9241 aggaggcggc atgcggttcc tgacggtgga ccaaatgcgc gctgcggatc gcgatgccgt
     9301 gaccgaagcg gggattccgg aacgcgcatt gatgcggcgg gccggcgagg cgctggcttg
     9361 cgcggtggcg cgcgtggcgg cacggcggcg gacgcgcgca gcggtgctgg tggctggcca
     9421 cgggaataac ggtggcgatg cctgtgtggc cgcgcgcgcg ttgcgggagg ccgggttcct
     9481 cgtgcgggtg ctgttgaccg ctccggtcgc gcggttccgc ggggttgccc gcgaggcgtg
     9541 ggaggcgatg gacgcggcgg gcgtgccgtg gtcggaaccc gaggccgcag agtctgcgtg
     9601 cggcggatgg acagaggaca atgcggatgc ggttgccgcg ccttccgcat ggggtggcgt
     9661 ggtggtggac ggagtgctgg gcacggggtg tgctggcgcg ccgtctggcg cggccgcgcg
     9721 ggccatcgga tggatcaacc ggcagcgtgc gcatgcgatg atcgttgctg cggatatccc
     9781 ttcgggtgtg catggcgata gcggcgaagc cgcaggtgtt gcggtgcatg ccgatgtgac
     9841 cgtcacgttc gcgcgtccca aacgcgggtt gctcaacccg tgcggcgcgg agcgcttcgg
     9901 acatgtgatc gtggctgata tcggtctgcc ggatgcgttg tgcgaccggg gtacggatga
     9961 tgcgccgtgt gaatgcatcg ctctgccgga actcgcccgg ttctttccgc gtcggcaatg
    10021 gagcgcccac aagggcacgt gcggtcatgt cgccgtgatc ggcggcgcgg caggaatgcc
    10081 gggcgcgccg gtgctcgcgg cgctcggcgc ggtgcggagc ggggcggggc tggtatcgtt
    10141 ggccgtgccg ccgcagagcg cggctgtggc tgcgacgtgg gtccccgagg cgatgtgtca
    10201 tacgctggac gtgccgcgcg gggtgatttc gcctcatgcg cttgccgcag gattttcggg
    10261 gctggacgcg tgcaaggtgg tcatcgccgg gcccggcttg acacggcacg aggaggcagg
    10321 ccgcactgtg gcgcaacttt tggagaccgg gccggaccgg ttgttgctgg atgcggacgg
    10381 gctcaacgct ctggccgatc tgtttcacgc tggctggcgt ccgcgcgccc ggcaggatct
    10441 gctgctgacg ccgcatccgg gtgaggcggc gcggttgctg ggtctcagtg tggcggcagt
    10501 ggaggcggac cgcgtggcgg cggtgcggtt gctggcggaa cggtaccggg cggtggtggt
    10561 gctcaaaggc gcgggtacgc tggtgtgcgc accggacggg cggccttggt tgaaccggac
    10621 cgggaatccc ggcatggctt cgggcgggat gggggatttg ctcgcgggga tggtcgccgc
    10681 attgtgggca caggggttgg atcccgtgca ggcggcctgc accgcagtgt gggcgcacgg
    10741 tgccgcaggg gatgaggcgg catgggaaga gggccgaacc gcactgagcg caacgcggtt
    10801 ggccgccgcg ctcggatcgg tttacggcga actggaacgg ctcgcggcag gatctgagga
    10861 agggggcggt aatgtcggat gatttgacgg atcgtcaggt gctgacgcgt ctggcgtggc
    10921 tgttcgcgct ttttgcggtg atcagcttgg cggcgggggc gagccggatg caacccccgg
    10981 ggtggcagtt gcggacgttc ggtgatgtgt tgcggttgcg tgaggcgctt tcgatgtttg
    11041 tcttcgcgcc ggccctcggc cttgtgttct ggttgatcgg ccgtacgctc agtcggggcc
    11101 gtacggtcgt ggcggcggat ctgctgatgg tgcttgccgt gtatggggtg gcttgcggca
    11161 tgggaatcca tgatccgacc aatcgcatga tcagcgcgta ttcaggaagc ggcgcgctta
    11221 cgccagaggt gcggcgttcg ctgttctatc tggacgaggt gttggggcat tgggtcttct
    11281 gggccggctt tgtgctcgcc acctgggtgc tcggtcttcg tcaggcgctt gcgcctttga
    11341 aagagcggat gcaatggcct tggcgggtcg ggttgggcgt cgtggcggca gcgtttctgt
    11401 gggtgatggt cacgaacctg tgggatgaat atccccagac ccgggcggac ctgggtgtga
    11461 tcctgctggc cgcgttaggt ccgttggcgg cacatgtggc gttcggccgg aatgcgggtg
    11521 tgttgcggat gccgctgcat ttcgtgattt atccgacgta tttcggcagt gtcgcggtga
    11581 cgctgatcaa ctggctgatc cggtacggcc gggtataagg gacgggcggg cgcggcccga
    11641 cggaaaggag tgtcgtgtca tggcgatcag gagtatgacg gggttcggcc gcggcgaggc
    11701 ggaggaaaac ggacgcgggg tgacggtgga gttgagcgcg gtcaaccgca agcagttcga
    11761 ttgcagcctc tccctgccgc gcgagttgtc gagtctggac acgcggatcc agacgctgat
    11821 ccgaacccgg gtgtcacgcg gttacgtcaa aggggtggtg acggttgcga cgagcgtggg
    11881 ggaggctggt ggcaatgcgg cgggatggga cccggaagtc attggcaagc accttacggc
    11941 cctgcgcgat ctggccaagc ggttggggtt ggcggatgac ctgaccgcct cctcgctgct
    12001 ttgtctgccg gacttgctgc ggggcgcggc gccgctggag ccggagaaga tgtggccgtt
    12061 ggtggagcga gcgggaacgt tggcgttgga aagtctggat acgatgcggc agcgggaggg
    12121 cgcggccctc gaacaggatt tgcgtaaacg gttgacggta ttggaaaaga cggccgagca
    12181 gatcgccgcg cgcgcgccga tggtgcctgt gacctatcag acgctgctga cgaaacgtct
    12241 tggcgagttg ttgggggagg aagggacggc ggtcgatgcc gcgctggtgg cgcgcgaagt
    12301 ggcggtgttc gcggaccggt gtgatgtgag cgaggaactg acccggctcg gcagccattt
    12361 cgcacaggtg gccaagactt tggactcggg cggaacatgc ggccgcacac tggattttct
    12421 ctgccaggaa ctgttccgcg agatcaacac gaccggttcc aaagccaacg atgccgaaat
    12481 ctcccggcat gtgatcgcct ttaaggccgc tctggaggcg atgcgcgaac aggtccagaa
    12541 catcgagtga cggaacagaa aaagaaacgg ggcggagccg ttgcggcccc gtcccgtttc
    12601 tctttccgtt agcgaagggt tattcgccgt acacgatggt ggtgacttcg ttgtagagga
    12661 agaggatgtt cttcacatca tagtccacat gatggatctt ggtgatgccg ccgttccgca
    12721 tggctgcgcc gatcgaggcg tcacccgtgg caaaacagat aatgccggtg gctttggcct
    12781 ggccgcgttt ggccgggcgg accgtgttgt ccacgaccgg atccgaggcg acgtgatcga
    12841 ccgtgaatac ggccatgatg cttcctttca tgggcgagag gcatccggtg gtcatcagga
    12901 cgctggcgca aagcagggac aggacgagag atttcttcat aacaactcct taatcagttt
    12961 aaaccgtccg caccatgcga acggagtatt acgatcataa caggccggaa ggggaaaggc
    13021 aaccgtaacg ggtgtccctt tacggtacgc ggtatgtcat cgtcatgggc accgtagcca
    13081 aaatcgcggt gcccagaaat cacgcgcatc cgtcaaatgc gggacacggt ggtagaatcg
    13141 cctgccatca atctgaagtc attgcgtcca cctccgctca actctctgcg gaaacgtgac
    13201 ggcgtcgtgc cgaattgccg tttgaagatg atgccgaggt ggctggcgga actgaagtgg
    13261 aggcggtcgg cgatgtcctg aatcgggagc cgggtggatt tcagcacctc gcagacacgt
    13321 tcgaggcggg cagacatgat cgcgttgtga accgtgctgt cgagcacctt ccggaaacgc
    13381 atctccagaa gacggcgtga gaccttgagt ttctccgcaa ggtctccgac ggagaaatcg
    13441 gcggcggagt tgagccggat acaggtgagg gcgcgctcga cgagcggatc cttcacgcgc
    13501 agatctgcgg tggagagacg cgaaacgatg gaatcgaggg tgtacgtcac gcgaatcggc
    13561 ctatggcgac gccggaaccc ctcgtgcatc gcgcggtcca gcagcgaggc ggcttcgtag
    13621 cccgcctgaa aggggttcat cttgatggag gaaagggacg ggacacagga ttcgcagagc
    13681 agttcgtcgt tatcacacga gagaatggag atttcctggg gaacggcgat gccgttctca
    13741 aggcaaaggt tgacgacctg ccgggcgcga gtgtcgttag cggcgaacag ggcaatgggc
    13801 ttcggcaggg catcgagcca tctggcgagc gtacgctgtt cgcgtacggc attgcgcgcg
    13861 acggaaatcg gcggcagcgt gtaatgatgg cactcgaaac cgcgtgcggc tacgcatcgg
    13921 gcgaagacct cgccgcggcg aatcgaccat tggggacgat tgacgtcgcc gacgtaggcg
    13981 aaggcggtga aacccttgga caggaagaac tcggcggcgg cacgtccgat agggacattg
    14041 tcgcattcga tgagaccgat ggtgttggac gggtagtcga ccggacggtc gtcctcgttg
    14101 ctggcgcggc cgttgagtat gagcgggatg tcggcgggga ttttcatgtc tacgaactgt
    14161 tcgtcggaga ggttgccgat caacccgttg catccccatt ccacgaggtc ggggagcttc
    14221 tgttcgagat tcctgccggc gaggatgtgc aaggcccagg ggccgttggc cttggcgtat
    14281 tcgagcacgc cctgcatcaa ctcgcgcatt gtcttgatgg cggccgagaa gcagatgaca
    14341 acccggggga tctcggttgt gagcaccatg gacgtcagaa tatcaagatc ctgtgagggg
    14401 aacaaggttg gctttcgttt ttttgatatt ttattttgcg cattacgaca tggtggcggg
    14461 aaatggaatt tgataaactg caactaaatc atacggacgg tttcgtcgat ctggcttggg
    14521 tgatgggtgt tgccttggtg gtgaccggcg gtgaccaatc agaaaggacg tcaagatgaa
    14581 gagagtggtg gggatgacgg cggtgtttag cctggcgttg gcggcggggg ggggccgagc
    14641 cctcgatcct gacgaacagc gtgacgatgg cgcagagttc ccagacgcac ttggttacga
    14701 tcggctacga gctgacgggt gcggacgcga tcgtgacact ggatatcaca acgaacggtg
    14761 tttcgattgg cgcggcaaat atccgcggtc ttgtgggcga cgtcaacaag cgtgtgtcgc
    14821 cgggtcagcg cgccatccag tggaatccga acaagtcgtg gaagaacaat gtcgtctccg
    14881 atgggtctgc gaaggcggtg gtgaagatat ggagcctgag cgaaccgccg ctgtacatga
    14941 cggcgagtct ggacacggcg aactgcgtgc gctactacga ctgtgcagac gcaatcgagg
    15001 ggggtattac gaatgacatg tacaagcgta cccatctcct cctgcgcaag gtcccagcga
    15061 caggcgtgac gtggcgtatg ggcgagaact cacgcaactc gggcgaatac acgggagcca
    15121 acgaaacgcc gcatctcgtg gcgttcacga acgactacta catcagcgtc tatcaattca
    15181 cccagggcca gattgcggaa cttcgcggtc ttggctgggt gggcggctgc aattacaaca
    15241 aagcttccca cgacgactgg tggagccatc cgatggagag catcaactac gactatctgc
    15301 gtggcgcgac gaagggcgcg cagtggccga caacggacga attcgatgcg gattcctgct
    15361 tcgccgttct acagaaccgg acgggcatgc gcttcgacct gccgacagat gcgcagtggg
    15421 agttcgccgc gcgggccggc aaccaactgg cgtcctcgac cgtggcggtg gatacgtata
    15481 cgtcgggttg ggagatcatg aatccctatg cctggacggg ttggaacagc ggtgacaacg
    15541 gcggcagcga aaacaagcgg actcatgcgg tgggaaccaa gttgccgaac aactggggaa
    15601 tctacgatat gcaggggaat gtgtgggaga tgtgccgcga ctggttcgag caggtctgcg
    15661 cgtttccggc ggacggttcg ccggtggtca atccgctcgg ccccatatcc ggatcaacgc
    15721 gcgtgatgcg tccgggcgga tacgacaact ccacgcacag cgtccatcgc tacacctttc
    15781 gtaaccatat cgatccgtcc gggagtgcga gtggttcggt gggattccgc gttgtgtgcg
    15841 agatcacagg tgaatgagga gggtgtaaaa tggaaaatgt aaaagaaaaa atggtggcag
    15901 tcgccgtgtt tatggcgatg gcggcggtgg ccgagccggc gtggacgttc acgatcgagg
    15961 atcatcctgc cgaggtgacc gcgagcgcga cgacgacgga cgcggtggcg gtggcgtccg
    16021 tcacgtcgca ggaggcgccg agcggtgcgg gtggactgac cgcgttcgtg tcagcgtttt
    16081 tcgagtcgct cggcatcaat ctccggagcg atccattcgg gacgctgttc ctgttcaagt
    16141 gaggcgaggg gagaattcaa aatggaacgt gcaaaatgga aaatggcggc ggcggtcgca
    16201 gggcgacgag tcctgccgtt gctggcgttt gctgcattca cggcgttggc ggcggacccg
    16261 gtcgtcagcg gtgtgacgat gacgcaggac gcgacgacga agaacgtgac gatcggctac
    16321 acgctttcgg gcgcggacgc gattgtcacg ctggacgtga cgacgaacgg cgtctcgatc
    16381 ggtggtgaga acctcacgaa ggtctggggc gacgtcaaca ggaaggttgt gaccggcaca
    16441 cggtcgatca tgtggcgtcc ggtcatgggc accgcagaca tgaagccggt ggtcacggcc
    16501 tggcgcacgg atacgccgcc cgactacatg gtgatccgca tggaccgtcc tcagggggcg
    16561 acgaacgtgg ttccgcttta ctacgcgaat acgaattcga ttccaggcgg cttcaataat
    16621 gctcggtaca agacgacgca catgatcttc cggaagtgtc cggcggccga ggtcgtgtgg
    16681 cggatgggaa cgccctcctc cgacgagttt ttcgaggtgg atcaagtggc gcatctcgtg
    16741 aagttgacgc acgacttcta catcggcgtc tatccctgca cgcagggcca gttcaccgcg
    16801 cttgcggggg ttggatggcg cgggcggtgc ggatacacct actccacaaa cattgattgg
    16861 gcggtccatc cgatggagag tatcaactac gacgatcttc ggggcaagac ggctggggcg
    16921 caatggccga catcgggcga tgtggacgcc ggttcgttct tcggtgtact gcgcgcgtat
    16981 acgggaatga acttcgatct gccgaccgag gcgcaatggg agtacgcctg ccgtgcggga
    17041 acgggtacga cggggaatat cggtgtcagc tacgggacct gctgggacga agtctcgtgg
    17101 atcaactaca acgccgacgg gcacacgcac gaggtggggc tcaagcggcc gaacgcgtgg
    17161 aacatctacg acatgcacgg acaggtgatg gagatctgcc gcgactggta cggcccctac
    17221 gaattcgtag acgacgagac gccggtagtg gacccggagg gcgcgccatc gggcgaatac
    17281 cgtgttgcgc ggccggcggc cttcaccaac aaccacacgc cgaatcacag cggtttccgg
    17341 aacaagatga agccctccga aggtgatatg ggcggatggc actggaacgg gagcacgtac
    17401 ttcgccggtt tccgtgtggc gttggaaatc gaatgaccgg tggttttcgg tgaagagtgg
    17461 agaacggaga gtgggaaatg gaaaatggaa aaacggtagg atcgtactct tcattctcca
    17521 ttctgcgttt ctcattttcc atttgaacgc atttccgggt ccagaggggc tggtggcgcg
    17581 tgtgacgccg gagtgggccg ggaaagtcgt gttcgaggat gtggcgcgaa aggcggggga
    17641 gaaggagttc tgcgaagtac ggtccctgcc ggacgggcgc atcgcggtaa agggcaatac
    17701 gccgaacgcg cagtgtgcgg gatacgggaa gtatctccgg aaggtggcga aggtcccgta
    17761 tttctgggag ggcggcggca cggcggcggt cgcagggcgg cacgcccccc cgaggatgga
    17821 tggcgtgatt cgggcggagg cgccgtacca atggcgggcg gtgttcaatt tctgcacgca
    17881 ttcgtattcg ctcgcgttcg cggacaaggc gcggtgggag cgggaacttg acctgcttgc
    17941 gctcaggggc gtcaatctgc cgctcgcctg cacgggcgtg gagtgcgtgt ggtacgagac
    18001 gctgatgtcg cttggatgga gcgacgagga ggcgcggagc ttcctcgtgg gcccggcctt
    18061 catggcgtgg cagtggatga cgaacatcga gagctatgcg ggaccgttgc cgaagagttg
    18121 gatcgaaaat cgtctcgcgc tcgcgcgttt tattctcgcg cgcgagcgcg agctcgggat
    18181 gacgcccatc cagcagggat tcacggggaa cgtgccgcgc gatacggtga agaagttccc
    18241 gtccgcgaag atcaagttcc agccgccctg gtgcgggttc ccggggtcgg cgcagctcga
    18301 tcccctcgac ccgctcttcc cgaaattcgc gcgggcgttt tatgccgcgg aaaagaagct
    18361 cttcggtctg cacggatact acgcggtgga tcctttccac gaatcgcagc cgccgcaggc
    18421 gggggacgac tatctcaggg cggtgggcga gacgatcggc cgtgagctcc gcgcagcgga
    18481 tcctgcggcg aaggcgtgta tgcagagctg gtccatccgc gtgccgattc tgaagtcgtt
    18541 tccgaaggac ggcatcattg tcctggacat ctcgggcgag aagggccgcc acaccaaagg
    18601 tttctggggg tatccgttcg tgacgggggc aatcataaac tttggcggac ggacggcctc
    18661 gggcggcgac atccgttggc tcgctgaaaa ccacttcgcc gagacatacc ggaagttccc
    18721 gaactgcgtg ggcatggggt attttccgga gggcatccac acctcgccgc tctactggga
    18781 gcaggcactc gacctcttgt ggcgggatga caaccccgaa ccactcgcct ggacgacgga
    18841 actgctcgag gcccgctacg gcgtgccttc cgctacggcg aagagccttg ccgaaggact
    18901 tctcgcgacc tattatggac gggtccatct cgcgtccgcg gtctatccgg ccaaaccgac
    18961 cttcctgctg aaacgctgcg atccgaacgt gggattcagg tccggatacg accatcggcg
    19021 cctcctgcgg acatggaggg agatgattac ggcggcaaag gaattcggcg tgcgtccagg
    19081 catgcggttc gacctcgtag acgcggggca tcgtctcttg agcgatcttt cctacgcgca
    19141 gtttgtggac gtggtcgaga tgttcctgaa aggggacgtg aaggaatacg actgcgcagt
    19201 ggcggcgttt ttggagacgg gggcggacga ggactggctc cggaagcatg agccgctctc
    19261 ctcattgaag acgtatcttg atctggcggc cgcacagggg aagacggacg aggagcggaa
    19321 tctctacacg ttcaatcagg cggtgcaggt gacctactgg gggccattcg gcgtgccgac
    19381 ggtcgccggc gatccgattc aggagtatgc gtggaaggaa tggggcggac tcctctcggg
    19441 gtattacctg ccacggtgga agatgttttt ggaacatgcc cgcaaggtgt tggaggggaa
    19501 ggcggaactg gcagacattg tgccgccgac ggacatctgg caacgtccga agcatcgagg
    19561 aaacgcgttt tactcgaaga tggcggattt cgaagagggc tggatccgcg atccgaagcg
    19621 gatgaccggc gatgctccga cggacgatgt cgtttccgcc tgcgagaaag tgcttgcgaa
    19681 gcaagcggcg gcattcgaat atcaatttga aaatggaaaa gagaaaatca acgggtttga
    19741 ggcgaaaatc gcggagttcg aaaaggagat ccagcgccgg atcaaggcgg ggcgcgtgcg
    19801 tgccgacgtc acgggggacg ggaaggaaaa cgacaccgcc gatcttatgc ggcaagtcgg
    19861 tgatcaaatg aaagaagaga aatgaaaagt tcgaaaatca tgaattcctg tacgtttgtc
    19921 gtactcgtcg tttccccttt tgcatgttcc ctttcacatt tgaacgccgc cgaattccgg
    19981 gtgctgccgg gcgatgacgt gaaggcggtg gtcgcgcagg cggacgcagt gaggcggcag
    20041 aagccgacgg agccgatcga catcgtcttt gcgaagggcg accacgagat cacggtgccg
    20101 ctgtttctgc accgcacgaa gaatccgatc ggcgacgggc aggcggcgac gatgttccgc
    20161 ggcgaggagg gggcgcgggt gctgggcggt acgcgcgtgg ggccgtggag gaaatgtgcg
    20221 ttcaacggac gcaccgacac atggtgcgcg gacgtgccga aggacgtgga gcggatctac
    20281 acgttccacg cggacattga gatacggacc gaatgccgtt atccgaactt cgatcctgag
    20341 catccggaga cgggcggctg gctgttcatt ccaggcaaga tgttttcgat gtattcactg
    20401 cgggagggcg agcggaagga ccgcattccg atgcgggcgg aggactggaa gacgtgggca
    20461 aaaccgtccg aagggcgcgt gaacatcttc ccgcgctaca actggtggaa ccggataccc
    20521 ccgatcgcgg cggcggacga ggcgacgcac acgctccatc tggcgggaga cacgggggag
    20581 ttcccgcgcc cggcggaccg cttctacctg atcgggctcc gcgaggagct ggacgcgccg
    20641 aacgaatggt acttcgacgc ggacgcgcat cggatctggt tcatcccgcc cgcggggatg
    20701 gacccgaaca aggtccgcac cgcgatcacg acgacgacgc agccggtctt caacttcaac
    20761 gacgtgggga acgtccattt ccgcgatctc gagatctgcg agggtggcgg cggcatcaag
    20821 ctctacgacg gttcgcgcaa ctgcacggtg acgcactgca cgatccacga cacgggctgg
    20881 ttcgccggat gcggcatcca gacgtggggt tcgggcatcg tgatccgcga atgccgggtc
    20941 tggaacacgg gcaacagcgg cgtgcaggtt cattcgttgc gggatgcctc tcctgcggca
    21001 tacgctccgg ggcacgtgga cggcaacgag gtgtcgttct gccacatcca ccacacggga
    21061 cgcatcaacg gctgtggaga gtgcatatgc ggcgcgggac agggcacgcg gatcgtgaac
    21121 aaccttctcc accacgcgcc gcgcggagcg atcagcttcg gcggtcggat gaacgatatc
    21181 tcgcgcaacc gcatccacga cacgaacttc cagagcgagg acacaggagc gatctacacg
    21241 ggcggatacg tcaacaatgc ggggacgaag atcaacgaca actggatctc ggactcctac
    21301 gggttcagcc acggggggac aggtgtttgg gaacatcgca aggcggatgc gttcggtatc
    21361 tacctcgacg actcctccgg cggcgcggag gtggtcggga acctcgtgga acgctgcacg
    21421 cagggcgcaa tattctttca ttcggcgcgg ttcaacaccg tctccaacaa cttcttcatt
    21481 tcgaacggag gattcggcaa gtaccggctt cagatcacga tgaacggctt caccacgaac
    21541 gcgccgccct acctgaagac ggctgcgcag gaccagtacg accacatgat gaagggcggt
    21601 gcggcgtgga cgaactatcc gtcgcttcgc ctgccgccga cggcgttcga gcattcggag
    21661 gggatgctga tgatcgggaa ccagatcgtc aacaacgtcg tctactatcc cgaccagccg
    21721 gagtccttct acacggcgcc gggacagttc gcgccgaagg cgacgctctt cgaccgtaac
    21781 gtgatctggt gcgggaaggg gaacagggtg cgttacggac ggaacttcaa ggagatcccc
    21841 gctgagaaat gggcggagat gggtcaggac ctccacagcg tggtcgccga tccgctcttc
    21901 gtcgatccgg cacgcggcga ctactcgttc aagcccgatt cgccggcgct gaagctcggc
    21961 atccgtccgt tcaaggtctc gcactacgga ccgaactact gaagaatgtg aaaaggggaa
    22021 tgagaaatga agaaaggaaa acgggaaatg ggaaacgtca tggtgatgtc tgattgtttg
    22081 aatacggaga gggcaatggg caggactgtg cgtcctgcgc gaccgtgggt gcgcggaacg
    22141 cgtgttcccg ccgtcgtgct cctcgtcctt agttttacat tcttcatttt caatccttca
    22201 tttgcgcagg gcgacgaggg gcatgctgcg acggtaacgt ggcgcatcca cgagaaccag
    22261 tggcggacgg aggaggagtt caagaaattg acgaagttct tcgacgagca tcctgtgacg
    22321 ggaaagatcg cattctttac gtgtccaagc cacgtgccgc cgaagttcga gcactttctg
    22381 ccgtgcctgc cgcaattgaa gaaggcgatc gcgcatttca aggcacgcgg acaccgcacg
    22441 ggcatcaacc acctttgcac gatcggtcag acggacgagg atctcgaccg ggcgatcgtg
    22501 atcgaagggg cgcagtatct ggtgaactgg aagggtgaga cggcgaagag tgcacggtgt
    22561 ccgctcgatc cggtgtggcg ggagaagtac gtgaagccga tatacacggt tttggcggag
    22621 acggggccgg acttcatctg gacggacgac gacatcggtc gactctcggg gcacggcgtg
    22681 ccgggtccgg ggtgcttctg cccgatctgc atgaagcgtc tccatgaccg gctggggtat
    22741 tctggcgacg tgaagggact caacgcgttc tttaccgacg cggaaaaggg cgaggcgcga
    22801 cggcgggcga tgatgacgct caaccgcgag gtgtatgcgg atctgctcaa gtacatccgc
    22861 gaggtcgtgc acgccgttga cccgaagatc gtgatcggcg tgatggattc gatcacggcc
    22921 tacacgggga acaactacga ggagaagttc cgtgcgctcg cgccgacggg gacggaggag
    22981 gtgttcttcc gtccgggcgg cggatactac aacgacatcg gcggactcgg cggagttctt
    23041 tccaaggcga acgagatggg ctacgaaagc gcatatctgc cggatggcgt ggcggtgaac
    23101 gaatctgaga tcgagacctt ccagtaccag ctccttcata aggcgaattt ctcgctgaag
    23161 acggagtcgc tcgcatacat ggcggcagga acgcggggca cggcgtggaa cgtgtttcct
    23221 tttgcggaca acgagaccta cgaggcggtg gcgccgcgga ttgagtcgtg cgagaaggtg
    23281 cgcgtagagg cggacgcgat tgtggcggcg tgcggacggg cgaagccgag gggcgtgtgg
    23341 aacgggcgtg gacgcgatta cttcgtggga aacaagaacg gtggacggac aggggaatgg
    23401 atgcaggaag agtggtggga gaaagggttc tggcagtccg aactgcagat gacgggactt
    23461 cctgccgcct accgcgagga ggagtgtgaa gtgtgcgcgc cgaacgcgca ggcggtgtgg
    23521 tcttggacga aggagcaact cgaacgccgt ctcgcgggcg ggctctacct gccgctcgac
    23581 gcggcgcagg cgatcatcga ccgcggctac ggagaagatc tcgggttcaa gatcggcaag
    23641 gcggtttcga aggacgcgcg cgaacgcttc gtcgaccatc cactcaacgc gggcgtggcc
    23701 ggattccgac gggacatgcg gcagtcattc tggggcgggg acgtgattga actcgagccg
    23761 atggaagggg cgcagattct cgcggacgcg gtggatatcc cgggacgtgt cgtcgcgaag
    23821 tgtgtgagcg gcgtgtacga gaacaggcgt gggggacgga tcttcatgac ggggacgatg
    23881 gcctgggatc ggctgtcgtt cagtccggtg gtacgcgaac gcaagaacgt gatgcggtgg
    23941 ctctcgcggg agacgctcac gggatacatc gacagctacc acaaggcggc gatgtgggta
    24001 cgcggtgacc gggcgatcgc gatctacaac atgtcctttg atgcggcgga gggaatggaa
    24061 gttctactca agggtgaagc gtggtcgaag ggaatccgtc tttccggcgg cgatggcacg
    24121 gtgatcaggg gtgtgcggga aggcgcgtac acgcggtacc gtctgccgaa actcgccgcg
    24181 tggtccgtga cggcgtgggc gcgaatgtag aattgacacc atgttgacac ctgtatgctt
    24241 gaatgccgtg ctgacggagg caatgtatgg aaacgccggc aggacggttt cgatgaaacc
    24301 gtcgtggcgg ctccattcat gtcgccctgc cgttatcgcg tgtttctttt tccttttctc
    24361 tttttccatt ttatgtgatg ccttgggtgg aattgtctac gatattgact tcgggtcggc
    24421 gacgaatcag gtggcgcgcg atggcgtggg gaagataaac ggggtactgc cggatgactt
    24481 tgcggagaac tacacgggct ggtgcgacgg catcgcgacg acggaggtga agagtgagga
    24541 gggcataggg aagtatctgc gcctgacgac gtccgcgcag acgaagggcg gaacgcaatt
    24601 cgtggtcccg aaggaactga aaccgggctg ttaccggttc acggtcgagt cgcgcacgct
    24661 tggcgcggtc acgtatcgct gttcgtttcg gatgggcgga tcgccgtatt cggattgtct
    24721 cagtcggcac gtcatgagcg agaactggag cgtgcagagt tggtgcttct ccgtgccgaa
    24781 ggactggaag cagttgccga tgctgttcgt gaacacgagg ccgggcgtgg tagacctgaa
    24841 gcggatgaag gttgaaacgg cgacgatagc ggagatggcg caggaggcgg tgcggcgtcc
    24901 gtcgaaggac gtgacggagt acattcgcca caaccgtttc ccgttgggct tgccgacagg
    24961 gtggtgtttc cggcgggatt gcgagggaac ggtgtacggt gcggacgaga cggaaccggc
    25021 accggacggc gtgccggtgt tgaaggtttc gggcaaccgc atgtggacct tctattccga
    25081 agcgttccag actgcgtttc cgggtggcga gatggaggtg tcgttcaagt accggagcga
    25141 ttgccgtgtg cggaatttcg tcaacgacga ccactggcgg ggagagatgt tcggcgaatc
    25201 ctggctcgga ccgacaaaag agtggaagga gcatcgcttc cgattcaggg ggcccgaact
    25261 cgcggaggcg ttcacgatcg gatggggaac cggtgttgag gcgggaacga tgtggcttga
    25321 tgacgtgcgt gtacgaccga tggacggcac aggtgtacgg gatacgcgtc cctgcctgca
    25381 gatggcagtg gtggaaggcg atgcgggaga aggaacgcgg gtacagttcc tggacgagaa
    25441 gccgctcgta aaatggtgtg tggtcgatgt gccgacgggg tcggtgatga aagtggaggc
    25501 ggcggatctc tacggcgaag aggtgacgat tgcggagatt ccgctcaagg gcgggaagct
    25561 tgagaaggga acgttggaaa tatcggcgtt caaggactgt ccgatggggc agttccggat
    25621 ttgcgcgacg attgaaaagg acggcaaggt cgtgtcggag agggaggaaa tcgtccttac
    25681 gcgtgtagtg cgtccggtga tgtgggggaa ggacgcgccg aattcaccgt tcgcaagcca
    25741 cttcaatccg tggcgtcccg tggtgaagat gatgaaggcg tgcggggtga actgggtacg
    25801 gttccatgac gcggcggagg atctttccaa ctggttctat ctcgaacgga agaaggggga
    25861 gtggacgttc cgggacgcgg cgatcgacga gtaccgcagg gcgaatgtca aaatcttcgc
    25921 gcaactggga atgtcgcccg aatgggcgaa ccattcggca gacttggtga aggagaatcc
    25981 gaagtggacg ccggggtatt tcgggcgtta tctgcgtccg acgaattcgg ttgactgggt
    26041 gaactatgtg acgacgttcg caaaacacta tcggggtgtg atcgacgaat acttcgtatg
    26101 gaacgaaccg ggcggacaca gctggcagtt caacgacgac gcgaagtggt tcgcaccggg
    26161 tcgggcgggg caggacttcg ccgtactgca acgccagacc tacgacgcgc tgaaggcggt
    26221 cgatcccaag ttgaagctct tcgggttcaa tgcgggcggt ccgaaggaat gggtcgagga
    26281 cgtgatggac ggcggtggtt tcgactgctg cgacggtatc gactggcact actactccgt
    26341 gccgtccctt gcgcaccgcg agacagcgaa cgtgatggaa aagtattcgc tgccgtcgat
    26401 ccgtgcaaag tatccggatt ttggcgggaa gccgctttac atgagcgagg ggcaggggac
    26461 gaacgacggc gggagcaagc tgcggtgcca caacaccgga ctctatcacg cgaccgtgcc
    26521 gtggacgccg gatacgatgc aggattatct tgagctttcg gacaacacgg cgcggtttct
    26581 cgtacaactg ctggcggatg gcttcaaaaa ggtcttcctc tacacctgtc acggatacgt
    26641 gcatccggca cggcgcagcg agtttagcgt gctgattggc aaggatggct atccggtgcc
    26701 gtccttcgtg gcgcacgcgg cactggcgca gacaatcgag ggcaagacgt ttgtgaacaa
    26761 ggaagatttc ggtgagaagg gatgtgtatt caccttccgg gcgaaggacg gaaagaatgc
    26821 gagggtgtat tccgaccttt ccgagaagga gaaactcgcg cttgcgaaga agacatgcct
    26881 gcatgatgtc ttcgggaatc cggtgacgga gaagacggca atgccggata cgttggtgta
    26941 cgaaccggga aagtaaacgg gggcgacgat gaaaaagttc cggttgatgt caa
//
LOCUS       DAOSRB010000014        26471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2033_length_26471_cov_6.640142, whole genome shotgun sequence.
ACCESSION   DAOSRB010000014 DAOSRB010000000
VERSION     DAOSRB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 26471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26471
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_26471_cov_6.640142"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2250
                     /locus_tag="PLZ74_01620"
     CDS             <1..2250
                     /locus_tag="PLZ74_01620"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOR97051.1"
                     /translation="PDRSHAAERFAADELSRWIGEMTGTYVPVRNETTATAGRPKLYV
                     GTDFAKDAFAADFKVIGKTDGFSVRTDGTNVYVFGTQPKGTLHAAYALLERNSDIIWP
                     RPDPTVGVVHSLTDSFVVVHADFREVPRTRVREWQWNYTGRGGAPNEALWGARNRQNQ
                     SGTPEGPLASCFVSAGKGHGLQRYARPDTNFSVHPEWFPLIGGVRTRKGGQLCMTASG
                     LADEIYASVTNELAQKYPDVSAKRIRVDYFNLTCADNHQVCECAACSSPFRCENGNVV
                     PISDPCFRSAQYYALMNQVARLLAKTHPRVTIGTYAYALTRYAPPFELAPNISVELCL
                     TDYDERASLTDPVRNLSARRHIDDWGDKCRGLWLRTYCGWSGRNLRAIEYAYAEACRY
                     AAKRRFPLAQYSAEIQRDEEVTGRHGAEIWDTSAISYWVISRLWWDPFQDVDVLRREF
                     VMRTYREAAPQMQAYFDAVRDAYAVDRMPATYRITCTAFMTQHYLYEPGLGPKLLGLL
                     DEALAIARHPVSREIIRRQRCRLQVNLDRAAALPTSRLAVRPLADGWGVAEVSRPFVG
                     AAGIYGASSDKYGGPAEVKTTFQAMHDDQALYLRVRCEAPDARTIYGASGNGSPEEQP
                     DCDDALEIFLCRPDTGAYWQWIMDPGAKGGPDIVFDAKGDDARWRGNWTRKVERDDFA
                     WTMTMRVPFADMAFEGCPDELRVNVIRARSPLNPPEEGKRKPRHFRSSWTGAGVHDSG
                     GFGLLKFVK"
     gene            2282..4903
                     /locus_tag="PLZ74_01625"
     CDS             2282..4903
                     /locus_tag="PLZ74_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOR97052.1"
                     /translation="MKKCMILFVIAAAFSVPAETWYIDAAAAKAHATAKSKPFDVPSV
                     WTNAAGDTASALLATDTLVVPDGVQLNYSGSMTAPAPLHLGVAGGGALAILHCSSGNL
                     TADIYWHNGRIQTQQYRASYGVTGTITVDGSEMGASHIWCYNKVQSTSTYNMSWNIWS
                     QLIGNAQQAIQFLYAPGTIKSEPVPIQQLPVVGNNVQWQGQFVVSAYANLILGHANAA
                     GSAETVRADAIVLGDHARLAVPVGVTPNAARGINITGSDVRFQARTYPHGFTDCTDYA
                     LNMPIAGTNGFTKTGAGRVVLGGAYTAGAIVVEEGTLEIVATATVTNGTQILVKNGAR
                     LITHISLAQVDVTVEEGGVFERVIDPFVVAFDSENVTTTPCVLSNEIVELEQIVPFRL
                     SESIPLPQHAAHKLAIFTYDGAETLMPPMFADTTPKTYGLPKGEITIESASGIQTVYL
                     NTRPVVVSRAKIEYASTNNINGSAETWSDGQTVHSNADYLLTHGYDSYHGSANVAFAG
                     DSLTLSETSFVSKASMNLFETASGNPLVFYPPFSSVQAHSGGRRHVYAGKIFLAGTYD
                     SEDAFTLETTYQDPARRTGTTTGSAELRADLTGEGVLFITARRLAGLNRLTGDNSGYR
                     GRILIQGNINDSDDGLFIEFGVAENLGGPLDAFHADALTLDQHAFLYPTTNVTLAADV
                     CRGMTVKDAGVLCPETTTLTTCWPLRLTGEFCKQGAGTLRLGGEISYGADGTGTNGTM
                     RVTDGTLSILSDAAVSGLAITFSNDVRLAVGPSLLRGLTVVPTVQDAVDGSAGHIRLA
                     IDAAAIPELWSGRIETVVATLPSGSADLTTMFGVPEHLRRYGVPDLVKRTVQIGDVTY
                     DQYVLTASCQKTVIIIR"
     gene            4912..6852
                     /locus_tag="PLZ74_01630"
     CDS             4912..6852
                     /locus_tag="PLZ74_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97053.1"
                     /translation="MKGFFLSRCRWLVPLVLAAEVWTAVSQEVRVSDFGFDAEDSTRF
                     LQAAIDSGAVRVVVDRQASPWVTEPLFCRSNQEIVFENGVEVVAKKGAFHGRNDCLFT
                     LKKVHDIKLTGLGDVVLRMHHDDYMSADYSRSEWRHVICILSSDRITLENLELRDSGG
                     DGLYVADARNGPASRDIVARRVISSGNARQGSSIISVEGLLYEDCVFANTRGRPPQAG
                     IDIEPNSGHNNVCGCIFRRCRFENNAGNGFQMSYPKEAKGRLPGSVRLEACVFRGNNY
                     GFSYGQNRKEPYYVKGEVSVTDCTFEGTRGPAIVISRKIGNSFKATVSNCRIENCCTG
                     SLNGSEIRINSLFGDFEPPSAIALKDIEIIQSTARDWIQFTNMPYGPHDKIDFTGNVL
                     VRSPAGVKRVAIDDAFRANAFPGFWNKGEPPRGKPMKEICTVVDSNPACSVKLTRLGL
                     RGTSRYVFYAAEAGPVIFIGSMKPASAVSPVQMRVVSAANGKEGRVLPMGQDGRLVVE
                     TAEPGLQTLTVLGDKEVFTLVESHVPVALEVAERPHALRVSSGDLFFYVPADRPFNVM
                     VCGESWGERVGAALYSPDGTEVWSEPGAIWWHGYRARGRVPEGLWRLNLRHPNKGVLQ
                     DYSVDMTGTNPYLFLSSEKYWK"
     gene            6875..7957
                     /locus_tag="PLZ74_01635"
     CDS             6875..7957
                     /locus_tag="PLZ74_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOR97054.1"
                     /translation="MKKTVPLILFLILALVVMGPWLPAQATLQACAGSGAQPCRDIVF
                     KPLPDGEMRYGDTSRIPDRPFAKDPTVIRHNGRYLMYYSVRGYPPESRPADVPKERWG
                     WCGAIAESTNLVDWVRVSDILFQGPARAVCCGVAPCVRKFDGKIHLFAQGPPLVGSGR
                     MRDVLWHATSTDGIHFTYGPENPVFVPQNEWSIARAIDAEVYRVGDRMMLMFATRERP
                     NARIQQLGVAWAPYGSDYGASKWTELSVKAPFFKPELPWEMHCIEAATVIVRKGIWYM
                     FYAGAYNHERQQIGVAWSADGVNFRRFRDTPILPHGPEGSWNAWESGHPGVFEDDDGA
                     VYLFYQGKRSLNGDYLLSCKEVEFVD"
     gene            7947..10031
                     /locus_tag="PLZ74_01640"
     CDS             7947..10031
                     /locus_tag="PLZ74_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97055.1"
                     /translation="MWIKKVLPFWEPPMLGETILVSGTAWRDEMNRCVIPFVCGSLFA
                     LSMRLEASPIARDAAIDPITTRWTFGAHEPYAMYRRVGRICTGAIDGNALWVQDWLDW
                     FDLHGPEKMEEMGFNFLHSRFYKGMGWEVEKKDFPNVKKFVANCHAHGVRALAYVQFG
                     TLYPEAMRRENPQIDSWAGVSETGGHNLYGGMYFRWMPCLNCREWEDYLKRMCSIALV
                     EGGFDGIMFDNVYDVPCYCQRCEKAFREHLKTVPDREARFGFDDLDYMMLPKIRISVE
                     TTLEVKDPVAQEWFRWRADVMTGVMTRLRDHIKHVKPDAIVAGNPSPYRNRAGALSKA
                     QNMAELCRAFDCIIMQNANFPEVREGGVIRNRVRDLKFAQDMGQRIVALCDNVEHLHA
                     DRESKFLLPMVEDLVFGGIPTDRTSIFPSPEPGFINRKVFEARKPIHTRFNAFATAHA
                     AELTAPAIHSVRIFYPEREIYLSERNHQGVVAAEEILLRNRVPYGYLVSSPDNVMTVP
                     PETEVVIVPGLVALSDAQVEALVAYAKSGGKLVVTGDAGRYDDWIAQRRANPLLPQLQ
                     GFANVVCRVEADLIRGAVMGWGYTVPPPVDGGKKLMEDLRKVGWRPPVSFDNLPPYVF
                     AEYRRLVSGGLVVHLVNYKPEERIVGARIALPKGVSAVLEEPFGNEPRKVTLEGDGVL
                     PTLGMYVMIVVK"
     gene            10524..12332
                     /locus_tag="PLZ74_01645"
     CDS             10524..12332
                     /locus_tag="PLZ74_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOR97056.1"
                     /translation="MILLACGILFASSMRLEAARPAFYLPGRLLAAPGPVCNTYHAAS
                     PTVDALEASLAARDAAVDAEWLAVPDKTAFVAKRTEFKTDFRRVIGYDEIQRTPLQAK
                     TTGVRVFDAFRIEKVLMESAPGEFIATLVFLPDAKKFAPPYAGFIFIPGHNDRGKGGK
                     DYLHTCELGARNGLAVITYDPLGQGERSQGAGLRSADEHVRIGAYAVLLGETTATYML
                     RDAVRVFDYFESRPDIDRNKLGVCGNSGGGTMAAFFMAVDDRVKAATPSCYLSSARKH
                     LLTCGPQDAEQNFFDAFSWGFNHAALMFAAECPVLINAAVEDFFQIEGSRSTHRLVKE
                     VAAKVGLPADWYELSEAPGPHKMSKTHRERSVSFLLKHLTGESRQVAETETTEFTADD
                     CTVTPDEEVSHLVGFKSIYDTIAEKFVARGVSVDNAAQNAKPLVAKELIGKDCSGVLA
                     TLKGELAKDRRATLRLGGLAGAGEVTATLFADAGRYVIRSERKGKRSYYERRGDDEVV
                     AVDLYIAGRSLVALRAAEILTLAAELEKRTGVKPMLVAEGRYVTIAKFALAADPTAFV
                     GVEYRNEPKSFLDSLKAREYLSFADSGALYSGKAGR"
     gene            12352..14361
                     /locus_tag="PLZ74_01650"
     CDS             12352..14361
                     /locus_tag="PLZ74_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97057.1"
                     /translation="MNRCVIPFVCGILFALSMRLEASTITRDVAVDPITTRWTFGAHE
                     PFTMYRRIGRRSTGGIYGGSKWVKDWLDWWDEHAPARMEEIGLNALHSRFYKGMGWEV
                     EKKDFPNVKKFVDNCHKHGVTVLAYVQFATLYHEVMQKEIPDIESWAAIDERGMKMIY
                     SRQAARWLPCIVNEKWVEYTEKILTIAMTEGGFDGVMFDNAFFFGCHCPRCQEAFRRH
                     IHSLPDLEERFGFAEMPGLRIPQAFRPENGDEYDPVAQEYYRWRVQCVNAVMKRFYRH
                     IKSLKPDAIVSANSGSFRSTDNYHARSVDVIDMADGGLDLLMMQTLNFPAYLDDPKFP
                     GQHYIVNRQRDLRMARDLGKQIVALSDGAAGSDALDESMYLRPLVEDLVWGGIPTDRT
                     VMSPVRREGFIDEERFAMRKKVLAEFDAFVKANRAAFTGKPYLPVRVFYPSAACGLAV
                     SMNLAVSTVDEILLRHHMPYGYVIVKGAEAPSIPADCEVLVLPNSKWMSDAQVSAVVD
                     WAKKGGRLVVTGRDTGLRDENGAERFTNPLDCVRKLPNVDWRNDIDVAQKNALGWITR
                     VLPPKDGGRAFMAGLGRVGYRNRFVFENLPEEVFVDMREVSDGVVIHLVNYHPEVQIK
                     GARLVVPSGAKATAEEPFGTRRQAVPLGKDGMLPAFGQYLMVRIY"
     gene            14384..16162
                     /locus_tag="PLZ74_01655"
     CDS             14384..16162
                     /locus_tag="PLZ74_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97058.1"
                     /translation="MKKGTLALLGALTLGLNVRADTSPDPTGGYDISVDYSLVAGLVE
                     YTNIYNQTAWTGMDPGYLTSPSQGVTSLWIRVSTDSNANLYYEDGDSYMKMLSYCGYI
                     WNNSPTNETWTFICCMDANSKLFIDGELKIDQNTRGYCKTGNVVMTPGAHAFEFRYRR
                     DGGNSGAIAANIMQSIDDEVFTGFAGNDGGEWKSKFGFAIDRQGRRSHNYRDYVSPVD
                     PGDGSLFTYTTNLNGIASLYVQQGAFRYPDPDVDYSRVAGLGEYTNLYNGVAWDATEP
                     CYLTSPSQGVTSLWVRVSTDANAHLYYQDGDAENKMLSYCGYVWNDSPTNETWTFACC
                     MDAHSKLFIDGELKVNQNVRKTCRTGNVVMTPGAHAFEFRYRKLGGYSGAIAASIMQY
                     LDDEPFTSYVGNNGGEWKSKFGFAIDRQGRGSHNYRDYVSPVDPGDGSLFVVTTNRLE
                     VSSLENRIDFLAMKFAPNTVLDLNAEGLTTAVSSIEGLPTVTNGNLTVTGTWTIPAVD
                     IAAGTVLNASGSLTLSAGAMPIVSNVAALPRNTVIIARAAGGLFLPEGIDGLALDPKG
                     KFVLCQTSPTELAIKYSVQGLLIMYK"
     gene            16185..18158
                     /locus_tag="PLZ74_01660"
     CDS             16185..18158
                     /locus_tag="PLZ74_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97059.1"
                     /translation="MPTRKITSFALFTLLATGCAVAGTPVVIDVPAGTKLTVIRDQIR
                     ALSVAQRVQGVEVRLARGVFEMEEGLALEPQDSGTPDAPIIWRGAACGGTRLIGGRAI
                     PADVFKPDPERPGVLVADVSDWQANWPAKPTETVHPYPVPELYVDGKRMMPAGWPDPV
                     SGELDDWEDGWATFDKVVNPGTNGVDCGTFVYRGLRPDRWVNEKYAYVFGFWKTEWRA
                     DEMPIASIDDKEKTITLGGFHFYGLGGINSWSNRPRRWRVLNVLCELDTPGECYVDVE
                     ANKVYLIPRHPLDAQSQVRLCWQDEDIVSLRGVANVTICDLTIGPAWKNAVWGSAVTN
                     VVIRNLTISGTRLKGIEFSEADHCRIADCTMYDLGAGGVKVSGGDRRNLIRGENVVEN
                     NLIHDFSKIRMTYAWAIQTWGVGDVVRHNEMYNTIHEALNVDGNDCLIEYNVISNAVT
                     GTDDSGAFHCGRNISCRGNVFRHNLFANIGNPEFRAQAALYLDDGHSGWTIDGCIFRD
                     VFRNEDKRGFGAIHCNGGYSNVVRNCLFINCGRALGSRWVTDEIWNNWLKEPGWIRRL
                     KREVDIEGSVYQSRYPELKGVMQGFPRRHRMQRCEDNVVAHCREFKKGDNWVTNQWNA
                     VVNELPPGDVNAACRQLIPHFQPIPFEKIGRYR"
     gene            18273..19730
                     /locus_tag="PLZ74_01665"
     CDS             18273..19730
                     /locus_tag="PLZ74_01665"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97060.1"
                     /translation="MSITRRGFIGGVSASIFIGGCTTVKGPRKIAANAKVNVAIIGCG
                     LIAKTTNAPGFLQDPRCRVTSVCDVVEIAPDYFYGSRNTNLGVEDFVRADGRYRRDVC
                     GWRVLRDVVNAHYGDKACKVVFDWRTIINDPAIDAVCICTPDHWHAIMSIAAMKAGKH
                     VFCQKPMTLSLAEGQTMVRVAKETGVTFQVGNQGSTNPAYRLTQEIVLNGYAGKVKSA
                     TICIPACDHWAGHGRSAARLPRPAYLSEAGWNMWQGPARHWEDNAYIPSIHDPTCWRF
                     NSRYGGGMIPDFGAHEFDQLNRGLGTQFTGPVAVENVETDYATGTDRDIFSWPSEFKF
                     DFVFANGVRCHVRKIDKEAGWPRQTIFHCEKGDVGSYAGKGKVPECLKNFRESDLTDK
                     DIKLHRPPAERDGSKFHESDFLDGVLTGSQDVASTCEMGHRTISMAHIANICARMQLS
                     SLKWDPKSERFVGAYADEANRFLSAEYHNGWELGA"
     gene            20102..21124
                     /locus_tag="PLZ74_01670"
     CDS             20102..21124
                     /locus_tag="PLZ74_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97061.1"
                     /translation="MIALAAGYQHFEDGAPFAEIALRHPAVKEVYFPWVDEPSGRPKL
                     GYEEEDDPAELEAALQRDLGRLREAGMKLDLLLNANCYGEKAMSHEFEERIITILEQL
                     ISWRCKPEIVTTASPFVARTLKKVFPEIEMRASVNMRLTTLQAFKYLAPLFDSYYLGR
                     DVQRNLDTVSRFSDWCHVNGKKLCILANSGCLRNCPWQTFHDNLIAHSDAAMNVENVK
                     GWSPHLCWTLYKDPKNFPHILKATWVRPEDLYRFEGLVDVVKLATRQHANPDMVIGAY
                     ERGGFEGNLLDLFEPGFSPAFFPTALDNAAFPPDWFDRTSSCSRECAECGYCEQVLEK
                     VKVNIG"
     gene            21299..21874
                     /gene="rsmD"
                     /locus_tag="PLZ74_01675"
     CDS             21299..21874
                     /gene="rsmD"
                     /locus_tag="PLZ74_01675"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOR97062.1"
                     /translation="MRITGGEWGGRKLKAPAGDRVRPTQDRVREALFSMLMGAVPGAL
                     FIDLFAGTGAVGLEALSRGASRVVWVEQDPRYAAVLQENVVAVTGKPGEVCRCGVLRW
                     LKGAGCGMRADVVFADPPYAADGDSGFAQIMGLLETNRVLRPGGIFVAEMPEACAAEE
                     TAGWRLVRDRTYGHTRLAVYRMGTEGTETTD"
     gene            21917..22468
                     /gene="coaD"
                     /locus_tag="PLZ74_01680"
     CDS             21917..22468
                     /gene="coaD"
                     /locus_tag="PLZ74_01680"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOR97063.1"
                     /translation="MEKIVVYPGTFDPLTLGHFDLLRRSASLFDQVIVVVADVSVKAG
                     GMFNLQERMAMIRESVDEVGMTNIEIDRLDCLLVDYCRRRKVRVVVRGVRVYSDFEYE
                     FQMALTNRKLAPEIETLFMMPNENYSYVTASTIREIARYGGDTSAFVPAPVQKYIRAH
                     MSRHPDPHIRAGGGIGNTGAFDR"
     gene            22458..22916
                     /locus_tag="PLZ74_01685"
     CDS             22458..22916
                     /locus_tag="PLZ74_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOR97064.1"
                     /translation="MIVDVARLEAGGEAYCGETGTGLLELGESEWVTPVGGMRYALRV
                     EMAGDELLVRGEVRQSLRCVCSRCAVEFDTEVAVPDFLCSLERDTLDEFVDLTSELRE
                     ATILALPGYPVCRETCQGLCMTCGVNLNTASCSCRKQDRDRRWSVLDALS"
     gene            22965..23144
                     /gene="rpmF"
                     /locus_tag="PLZ74_01690"
     CDS             22965..23144
                     /gene="rpmF"
                     /locus_tag="PLZ74_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOR97065.1"
                     /translation="MAVPKRKKSKMRKNQRKGHIKAKVAQVQACPSCGASQQTHRVCA
                     SCGMYKGRKVLTVAE"
     gene            23245..24276
                     /gene="plsX"
                     /locus_tag="PLZ74_01695"
     CDS             23245..24276
                     /gene="plsX"
                     /locus_tag="PLZ74_01695"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOR97066.1"
                     /translation="MRIALDVMGGDRAPEEIVHGAVEASLKFKDVSKILMVGDEAAIR
                     AELAKYPKADLTRLDIVHAPDSISMGESPVVAVRKKKRCSINLCMDLIKNREADAMVS
                     AGNSGAVAAAAMFCLGRMPGVLRPAIATVIPTRQPKRPLLLLDAGANTDCHEAWLAQF
                     AVMGNAYSQAVLKRTEPVVGLLSIGTEDCKGNDMTKKAFHLIQRSKVKFRGNVEGHDL
                     FLGKIDVVVCDGFVGNVVLKTTESVAHAVGYWMKREFFRHPVRLLGALLLKGALETLK
                     RRMDPEIYGGAPLLGVPGTVIITHGSSSYRAIFHAIIAGAAAAANNLSDTIARRIAEM
                     EMPDEGAGV"
     gene            24443..25369
                     /gene="fabD"
                     /locus_tag="PLZ74_01700"
     CDS             24443..25369
                     /gene="fabD"
                     /locus_tag="PLZ74_01700"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOR97067.1"
                     /translation="MSDRVCVFAGQGAQVPGMGKDFASDPEGMALFECANRVLGFDLA
                     TLCFEGPAEALTKSNVCQPAIFTVSVAAFRAFQKRCPRVSFAMAGGLSLGEWTALHVA
                     GVLEFEAALTILEARGRFMQQACEEQASGMISIMGATAEQLQTLCDRTGLTVANINSD
                     AQVVLSGLKAGVESAVAVAAELGVKAIPLNVAGAFHSPLMASARSKLAEVLEGVTFRA
                     PSMPVLANVTGAPHSTDPSAIRDAMLRQITDSVRWLDCVRHAVAAGAGGFVEFGPGKV
                     LSGLIRRIDKGVDTLNVADVTSLDAAVAALGA"
     gene            25404..26141
                     /gene="fabG"
                     /locus_tag="PLZ74_01705"
     CDS             25404..26141
                     /gene="fabG"
                     /locus_tag="PLZ74_01705"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOR97068.1"
                     /translation="MLDGKVAVVTGAARGIGQAIARRLAQEGADIVLCDLQAEWLAES
                     AGMVQDCGRKALPLAVDVGDSEAVNACVQEAVKEFGKVDILVNNAGITKDTLLVRMSD
                     ADWDDVIRVNLKGTFLFTRAVAKNMMRQRSGAIINIASISGLIGTAGQANYAASKAGV
                     IALTKSTANELAGRGVRANAIAPGFISSKMTDALSEDVRKQYLARIPLGRFGTVEDIA
                     HAVVFLASEQSSYLTGQTLNVNGGMVM"
     gene            26205..26453
                     /gene="acpP"
                     /locus_tag="PLZ74_01710"
     CDS             26205..26453
                     /gene="acpP"
                     /locus_tag="PLZ74_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOR97069.1"
                     /translation="MASKLEERVKEIIVEQLGVNAEQVTPEASFIDDLGADSLDTVEL
                     IMAFEEEFGAEIPDEDAEKLTSVGKVFEYLKEKGFGQD"
BASE COUNT         5284 a         6926 c         8560 g         5701 t
ORIGIN      
        1 ccggacagat ctcatgcggc cgaacggttt gcggcggacg agttgagccg ttggattggc
       61 gagatgaccg ggacctatgt gccggttcgg aacgaaacca ctgccacagc gggcaggccc
      121 aagctctacg tcggcaccga tttcgcgaag gatgccttcg ccgccgattt taaggtcatc
      181 gggaaaacag acggcttttc cgtgcgaacc gacgggacga acgtttatgt tttcggcacc
      241 caaccgaagg gaacgctcca tgcggcgtat gcgttgctcg aacggaattc cgacatcatc
      301 tggccgcgtc ccgatccgac agtgggcgtg gttcattcgt tgacggattc gtttgtcgtc
      361 gtacatgcgg acttccgcga agtgccgcgt acgagggttc gtgagtggca atggaactac
      421 acgggacgtg gtggcgctcc aaacgaggct ctttgggggg cgcggaatcg tcagaatcag
      481 agcggtacgc cggaggggcc tctggcaagc tgcttcgtca gcgccggcaa ggggcacggc
      541 ttgcagcgtt atgcgcggcc tgacacgaat ttctccgtgc atccggagtg gtttccgttg
      601 attggcggtg tgcgtacgag gaaaggcggg caactttgca tgacggcgag cgggcttgcc
      661 gacgaaatct acgcttctgt cacgaacgaa ttggcccaga agtacccgga cgtttcagca
      721 aagcggatcc gagttgacta cttcaatttg acgtgtgcgg acaaccatca ggtttgcgaa
      781 tgtgcggctt gttcgagccc gttccggtgt gaaaacggga acgttgtccc gatttccgat
      841 ccttgtttcc gttcggcgca gtactatgcg ctcatgaatc aggtcgcgcg tctcctggcg
      901 aagacacatc cgcgcgtgac gatcggtacc tatgcctacg cgctcacgcg ctatgcgccg
      961 ccgtttgaac tcgcgccaaa tatctcggtc gagctttgtc tgaccgatta tgatgagcgc
     1021 gcatcactta cggatcccgt acgcaatctt tccgcacgtc gtcatatcga cgattggggt
     1081 gacaagtgcc gtggcttgtg gctgcgtacg tactgcggtt ggtcgggacg taatttgcgt
     1141 gccattgagt acgcctatgc tgaggcctgc cggtatgccg cgaagcggcg tttcccgctg
     1201 gcgcagtatt ctgcggagat ccagcgtgac gaggaggtga cgggacgtca cggggccgag
     1261 atctgggaca cgtcggccat ctcctattgg gtcattagcc gtctttggtg ggatccattt
     1321 caggacgtcg atgtgttgcg gcgcgagttc gtcatgcgca cctaccgcga ggcggcgccg
     1381 cagatgcagg cgtattttga cgccgtgcgc gacgcctatg cggtcgaccg catgcccgcg
     1441 acataccgca tcacctgcac ggcctttatg acgcaacatt atctttacga gccgggattg
     1501 gggccgaagc tgcttgggct tcttgacgaa gcgcttgcca tcgcgcgtca tcctgtttct
     1561 cgcgaaatca tccgccgcca gcgttgccgt ttgcaggtga atcttgatcg tgcggcggcc
     1621 ctgccgacca gtcgccttgc cgtgcgtccc ctggccgacg ggtggggcgt agctgaggtg
     1681 tcgcgtcctt ttgtcggcgc ggccggtatt tacggcgcaa gcagcgacaa gtacggtggc
     1741 ccggccgagg tcaagacgac ttttcaggca atgcacgatg atcaggcact ctatctgcgt
     1801 gtccgctgcg aggcgcctga tgcccgcacg atctacggtg cgtcgggcaa cggttcgccg
     1861 gaagagcagc ccgactgtga tgacgcgctc gaaatcttcc tgtgccgtcc cgatactggt
     1921 gcctactggc agtggattat ggatcccggg gccaagggtg gccccgacat tgtctttgac
     1981 gcgaaaggtg atgatgccag gtggcgcggt aactggacac gcaaggttga acgagacgac
     2041 tttgcgtgga cgatgacgat gcgcgtgccg ttcgcggaca tggcgtttga gggatgtccg
     2101 gatgagttgc gcgtcaacgt gattcgcgcc cgcagtccct tgaatccgcc tgaagaagga
     2161 aagcgcaagc cccgccattt ccgctcaagc tggaccggtg ccggtgttca cgactcaggt
     2221 ggtttcggac tgttgaagtt tgttaaataa ctatgcccaa aaacccaatc aggagaccag
     2281 aatgaaaaag tgcatgattc tgtttgtgat cgcggcggcc ttttctgttc ccgccgagac
     2341 atggtatatc gacgcggcag cggcgaaagc gcacgcgacg gcaaaaagca agccgtttga
     2401 cgtgccctca gtctggacga atgccgccgg agatacggcg agtgcattgc tcgcgacgga
     2461 tactcttgtc gtgccggatg gtgtccaatt gaactatagc ggcagcatga ctgcgccggc
     2521 accgctgcat ctcggtgtgg ccggtggtgg cgctttggct atccttcatt gtagttcggg
     2581 caatctgact gcggatattt actggcataa cgggcgaatt caaacacagc agtatagggc
     2641 gtcctatggc gtgacgggaa cgatcacggt tgacggatcg gagatggggg cctcgcacat
     2701 ttggtgttac aataaagttc agtccacatc gacgtacaac atgtcctgga acatctggag
     2761 ccagttgatt ggcaatgccc aacaggccat tcagttcctg tatgcgccgg gaaccatcaa
     2821 gtccgagccg gttccgattc agcagcttcc ggtggtggga aacaatgtgc aatggcaggg
     2881 tcagtttgtc gtcagcgcgt acgcgaactt gatcctcgga catgcgaatg cggccggttc
     2941 cgcagagacg gtgcgagccg acgccattgt tctgggtgac cacgcgcggc tggcggtgcc
     3001 ggtcggggtg acgccgaatg cggcgcgtgg catcaacatc acgggatcgg atgtcaggtt
     3061 tcaggcgcga acgtatccgc atggattcac ggattgcacg gactatgcac tgaatatgcc
     3121 gattgccggt acgaacggtt tcacgaagac aggtgccggg cgcgtcgtct tgggcggtgc
     3181 ctatacggcg ggtgcgattg ttgtcgagga gggtacattg gaaattgttg cgacggcaac
     3241 tgtcacgaat gggacgcaaa ttctagtcaa gaatggcgca cggctgatca ctcatatatc
     3301 gcttgcgcag gtagatgtga cggtcgaaga gggcggcgtg tttgagcgcg tgatcgatcc
     3361 gtttgttgtc gcctttgaca gtgaaaacgt cacgacaacg ccctgtgtat tgtcgaatga
     3421 gatcgtcgag ttggagcaga tcgtgccctt cagactgtcc gaatccattc ccttgccaca
     3481 acacgcagcg cacaagctgg caatcttcac ctatgacggg gcggagacgc tgatgcctcc
     3541 gatgtttgcc gacacgactc caaagacgta cggcctgccg aagggggaga tcaccataga
     3601 atctgccagc ggtatccaga cggtgtatct gaacacgcgt cccgtcgtcg tcagccgtgc
     3661 gaaaatcgaa tacgcgtcaa cgaacaacat caacggttcg gcagaaacgt ggtcggacgg
     3721 gcagaccgtt cattcgaacg ccgactatct gctgacacat ggatatgact cttaccacgg
     3781 ttcggctaac gtggcatttg ccggagattc gctgacgtta tccgaaacga gctttgtcag
     3841 taaggcgtcg atgaaccttt tcgagacggc atccggcaat cctcttgtgt tttatccgcc
     3901 gttttcgtcc gtccaggcgc attcgggcgg ccgccgccac gtttatgccg gcaagatttt
     3961 tctggctggc acgtatgatt ccgaggatgc atttacgctt gaaacaacgt atcaggatcc
     4021 ggcgcgccgt acaggtacca caacgggatc agccgagttg cgcgccgatc tgacgggaga
     4081 gggggttctg ttcattacgg cgcggcgcct ggcgggactg aaccgtctca cgggcgacaa
     4141 cagcggctat cggggacgca tcctgattca agggaatatc aacgactcgg acgacggact
     4201 tttcatcgag ttcggtgttg ccgagaatct cggggggccg ctcgatgcgt ttcatgcgga
     4261 cgccttgacg cttgaccagc atgcgtttct ctatccaacg acaaatgtga cgctggcagc
     4321 ggacgtgtgt cgtggtatga ccgtcaagga tgcaggcgtg ttgtgcccgg aaacgacgac
     4381 gttgacaacc tgttggccgc tgcgtttgac aggggagttc tgcaagcagg gcgctggtac
     4441 gctccgcctg ggaggcgaaa tctcctatgg tgcggatggc acggggacaa acgggacgat
     4501 gcgcgtgaca gacggtacgc ttagcattct ctcggatgca gccgtttcgg gtctcgccat
     4561 cacgttctcg aatgacgtcc gtctagccgt cgggccttcg ttattacgtg gactgaccgt
     4621 cgtgccgacc gtgcaggacg cggttgacgg ttctgcgggg cacattcgcc ttgcgattga
     4681 tgcggcggcg attccggagc tgtggtccgg ccggattgag acggttgtgg cgacgcttcc
     4741 gtcgggatct gccgatctga cgacgatgtt cggcgtgccg gaacatctgc ggagatatgg
     4801 tgtgccggat ctggtcaagc ggacggtaca gatcggtgac gtgacatacg atcagtacgt
     4861 ccttacggcg tcatgtcaga agacggtcat tattattcgg tgaggcgtgg cgtgaaaggt
     4921 tttttcctgt cccgatgccg ttggctcgtg ccgcttgttc tggcggcgga agtctggacg
     4981 gctgtttcgc aggaggtgcg tgtttcggat ttcggttttg acgccgaaga ttcgacacgc
     5041 tttctgcagg cggcgatcga ctccggtgcc gtccgggtag ttgtggaccg tcaagcgtcg
     5101 ccttgggtca ccgagccgct cttctgccgt tcgaatcagg agatcgtctt cgagaacggc
     5161 gtggaagtcg ttgccaagaa aggtgcgttt cacggacgca acgactgcct tttcactctg
     5221 aaaaaggttc acgatatcaa attgaccggt ttgggcgatg tggtgctgcg aatgcaccac
     5281 gacgattaca tgagtgccga ctattcgcgc agtgaatggc gtcacgtcat ctgtatcctc
     5341 agttcggatc gtattaccct tgaaaatctg gagttgcgtg attccggcgg tgacggcctc
     5401 tatgtggcgg atgcccgtaa cgggccggca tccagggata ttgttgcacg tcgcgtgatt
     5461 tcctctggca atgcccgtca gggatcgtcg atcattagcg tcgaaggact gctgtacgag
     5521 gactgtgttt tcgccaacac acggggacgc cccccccaag ccggcataga catcgaaccc
     5581 aactctgggc acaacaatgt ctgcggttgt atcttccgtc gctgtagatt cgaaaacaac
     5641 gcgggcaacg gttttcagat gtcctatcct aaagaagcaa aaggacgtct cccgggatcg
     5701 gtgcgactgg aggcgtgtgt gtttcgcggc aacaactacg gtttttccta tggtcaaaac
     5761 cgtaaggaac cctattatgt gaagggcgag gtgtccgtga cggactgtac gtttgaagga
     5821 acgcgcggcc cagcgattgt gatcagccgg aagatcggga actccttcaa agcgacggtg
     5881 tcgaactgtc gtattgagaa ttgctgcacg gggagtctga acggcagcga aattcgcatc
     5941 aattcacttt ttggcgattt tgagccgcct tccgccattg ccttgaagga tatcgaaatc
     6001 atccagtcaa ctgctcgcga ctggatccag ttcacaaaca tgccctatgg gccgcacgac
     6061 aagattgact tcacgggtaa cgtcctcgtg cgttcgcctg ctggcgtgaa gcgcgtggct
     6121 atcgatgatg cgtttcgcgc gaatgcgttc cccggtttct ggaacaaagg cgaaccaccg
     6181 cgtggaaaac cgatgaagga gatctgcacg gtggtggaca gtaatcccgc gtgttccgtc
     6241 aaactcacac gtttggggct tcgcggtacc tcgcgttatg tgttctatgc ggccgaggct
     6301 gggccggtca tattcatcgg cagcatgaaa ccggcctctg cggtatctcc tgtgcagatg
     6361 cgggtggtgt ctgccgcgaa tggtaaggaa gggcgtgtcc tgccgatggg gcaggatgga
     6421 cgactcgtcg tggaaaccgc cgagccgggc ttgcagacat tgacggtttt gggcgacaag
     6481 gaggttttca cgcttgtcga gtcccatgtg ccggtcgccc tcgaagtggc agagcgtccg
     6541 catgcgctta gggtatcaag cggggatctg tttttctatg tgccggccga tcggcctttt
     6601 aacgtcatgg tgtgcggtga gtcgtgggga gagcgcgtgg gcgcggcact gtattcgccg
     6661 gacggaacgg aggtgtggtc ggaaccgggg gccatttggt ggcacggata tcgggcgcgt
     6721 ggacgcgtgc cggagggcct gtggcggctc aacttgcgac atccgaacaa gggggtattg
     6781 caagattatt cggtggatat gacgggcacc aatccttatc tctttctctc atcggaaaag
     6841 tactggaaat aatgcaaaca aaagaaagac gaagatgaaa aaaacagttc cccttatcct
     6901 tttcctcatc ctcgccttgg ttgtgatggg accgtggctt cccgcacagg ctacattgca
     6961 ggcctgtgcg ggaagcggtg cgcaaccgtg ccgcgatatc gtgttcaaac cgcttccgga
     7021 tggtgagatg cggtatggcg atacgtcgcg cattcctgac cgtccgtttg caaaggatcc
     7081 gacggtgatc cggcacaatg ggcgttatct gatgtactac agcgtgcgtg ggtatccccc
     7141 cgagagtcgt cccgccgacg tgccgaaaga acggtgggga tggtgcggcg cgattgccga
     7201 aagcacgaat ctggttgatt gggtgcgcgt gagcgatatc ctctttcaag ggcctgcgcg
     7261 tgctgtctgt tgtggtgtgg cgccatgcgt ccgcaagttc gacggcaaga tccatctttt
     7321 cgcccaaggg ccgccgcttg ttggatccgg caggatgaga gatgtgctct ggcatgccac
     7381 cagcacggat ggcatccatt ttacgtatgg gcctgagaat cccgtctttg tgccgcagaa
     7441 cgaatggtcg attgcccgcg caatcgatgc agaggtctac cgcgtgggtg atcggatgat
     7501 gctgatgttc gccacgcgcg agcgtccgaa tgcccgcatc cagcagttgg gggtggcctg
     7561 ggcgccgtat gggtcggact atggcgcgtc gaagtggacg gagttgtcgg tgaaggcgcc
     7621 tttcttcaag cctgagcttc cttgggagat gcactgcatc gaagcagcca cggtgattgt
     7681 acgcaaaggc atctggtaca tgttctatgc cggagcctac aaccacgagc gccagcaaat
     7741 cggggtggcc tggtcggccg atggcgtgaa cttcaggcgc ttccgcgaca cacccattct
     7801 cccgcatgga ccggaagggt cgtggaatgc ctgggagtcc ggtcatcccg gtgttttcga
     7861 agatgatgat ggagccgtct atctcttcta tcagggcaag agatcgttga atggcgacta
     7921 tctactatcg tgtaaggaag tcgagtttgt ggattaaaaa ggttctgccg ttttgggaac
     7981 cgcctatgct aggagaaacc atattggttt cgggaacagc atggagagat gagatgaaca
     8041 gatgtgtgat tccttttgta tgcggcagtc ttttcgcgtt gtcgatgcgt ctggaggcat
     8101 ctccgatcgc acgggatgct gcaatcgacc cgatcacaac gcgctggacg ttcggagcgc
     8161 atgagcctta tgccatgtac cgtcgggtgg ggcgtatctg tacgggagcg atcgacggca
     8221 atgcgttgtg ggtacaagac tggcttgatt ggtttgatct gcacgggccg gagaaaatgg
     8281 aggaaatggg cttcaatttc ctgcacagcc gcttctacaa gggtatgggc tgggaggttg
     8341 aaaaaaagga tttcccgaac gtcaagaagt tcgtggcgaa ctgtcacgcg catggcgtgc
     8401 gggcactcgc ctacgtgcag ttcggcacgc tctatccgga ggcgatgcgt cgtgaaaatc
     8461 cccagataga ctcctgggcg ggcgtttcgg aaaccggcgg ccacaactta tacgggggca
     8521 tgtatttccg ttggatgccg tgccttaact gtcgcgagtg ggaggactat cttaagcgga
     8581 tgtgttcgat cgccctcgtg gaaggcggat ttgacggcat tatgttcgac aacgtctacg
     8641 atgtcccgtg ctattgtcag cggtgcgaga aggcttttcg cgaacatctc aagacggttc
     8701 cggatcgtga ggcgcgtttc ggtttcgacg atctcgatta catgatgttg ccgaagatcc
     8761 gtatttcggt agaaaccact cttgaagtga aggatcctgt agcgcaggaa tggtttcgct
     8821 ggcgggcgga tgtcatgacg ggtgtgatga ctcgtctacg tgaccacatc aagcatgtga
     8881 agcctgatgc cattgtcgct gggaatccgt cgccctatcg caaccgcgct ggtgctttgt
     8941 ctaaagcgca gaacatggcc gagctgtgcc gtgcgtttga ttgtatcatc atgcagaatg
     9001 ccaactttcc ggaggttcgg gagggaggtg tcattcgcaa tcgcgtgcgc gatctcaagt
     9061 tcgcacagga tatggggcaa cgtatcgtgg ctttgtgcga caacgtggag catctgcatg
     9121 ccgatcgcga atcgaagttc ctcctgccaa tggtggagga tctcgtcttt ggcggtatcc
     9181 ccaccgatcg cacatcaatc ttcccgtcgc cggagccagg gtttatcaat cgtaaggtgt
     9241 ttgaagctcg aaagccgata cacacgcgtt tcaatgcctt tgcgacagcg catgcggcgg
     9301 aactcaccgc accggccatc cacagcgttc gcattttcta tccggaacgc gagatctatc
     9361 tgtcggagag gaatcatcag ggagtcgttg cagccgagga aatcctgtta cgaaatcgtg
     9421 tcccgtacgg gtatctcgtt tcttcgccgg ataacgtgat gacggttccg ccggagacgg
     9481 aggtggtgat tgtgcctggc cttgtcgcgc tttccgatgc tcaggtcgag gcacttgtgg
     9541 catatgcgaa gtcagggggg aaacttgtgg tgaccggtga tgccggacgg tatgacgact
     9601 ggatcgcaca gcgtcgggcc aatccgctcc tgccgcaact gcaaggattt gcgaatgtgg
     9661 tctgccgcgt cgaagcggat ctcatccgtg gtgcggtaat ggggtggggc tataccgttc
     9721 ccccaccggt agatggggga aagaagctga tggaggatct ccgaaaggtc ggttggcgtc
     9781 cgccggtgtc cttcgataat ctgccgccgt acgtcttcgc ggaatacagg cggcttgtgt
     9841 ctggtggctt ggttgtccat ttggtcaact ataagccaga ggagcggatt gtcggtgcac
     9901 gaattgctct gccgaaaggc gtgtccgccg ttcttgagga gccgttcggg aatgaacccc
     9961 ggaaagtgac actcgaaggg gatggcgtac tgccgacact agggatgtac gtgatgattg
    10021 tcgtcaaata gatcgcatga aaatcatgac aaggggagga tcacatgatg aatagcatag
    10081 ttaagaatgc gatcgcggcg atttgcgtgg gcccgtgacg ctgggcaaga gtgcaaggtg
    10141 aaccttcaac tctctttggt ttcaaggttt cgcggtttca tccaaatcgg gatcgggatc
    10201 gctatcggga tcgaatgttg cgaccacgca gtggttcgca acgacgagtg taaaaatggc
    10261 ggcgtgaccg tcgccggacg agcagacgct cgttcctccc ggtgctttaa agcggcgtcg
    10321 cgcggaatgc cgcttgccgc cgcaaatacg ttcgcctagc ttgcgcccac acacaaaaca
    10381 cgcactcgtc tcgcttacgc cctgtcacca accgatcgat tacctacagc gggaggaatc
    10441 cattggacga actgcagttg aacgatgtat actatttcct atcggtttcg gaaacggcat
    10501 ggcgagatga gatggatgga cgtatgattc ttttggcatg tggtattctt ttcgcctcgt
    10561 cgatgcgtct ggaggcggcg cgtccggcgt tctacctgcc cggacggctc ttagccgcgc
    10621 cggggccggt gtgcaacacg tatcatgccg cctccccgac cgtggatgcg ctggaggcgt
    10681 ctctggccgc acgggatgcg gcggtcgatg ccgagtggct ggctgttccg gataagacgg
    10741 cgtttgtggc gaagcgcacg gagttcaaaa cggatttccg gcgtgtgatc ggctatgacg
    10801 agattcagcg cacgccgctt caagcgaaaa cgacaggtgt aagggtcttc gacgccttcc
    10861 gcatcgagaa ggtgctgatg gaaagtgctc ccggcgaatt cattgcgaca ctcgtcttcc
    10921 ttccggatgc caagaagttt gcgccgccct acgccgggtt catcttcatt cccggccata
    10981 acgaccgcgg taaaggcggg aaggattatc ttcatacttg tgaactgggc gcacggaatg
    11041 ggcttgcggt gattacctat gacccactcg gacagggcga gcgttcacag ggagcgggtt
    11101 tgaggagcgc agacgagcac gttcgcatcg gtgcgtatgc ggtgttgttg ggcgagacga
    11161 cggcgacgta tatgttgcgg gacgccgtcc gcgtcttcga ttatttcgag agccgtcccg
    11221 acattgaccg gaacaagctc ggtgtgtgcg gtaattccgg cggggggacg atggcggcgt
    11281 tctttatggc cgtcgatgat cgcgtgaagg cggcgactcc gtcttgctac ctctcttccg
    11341 cgcgcaaaca tctgctgacg tgcggtccgc aggacgcgga acagaatttc tttgacgcgt
    11401 tttcttgggg cttcaatcac gcggcactca tgtttgccgc agagtgtccc gttctcatca
    11461 atgctgcggt cgaggacttc tttcagatcg agggctcgcg ttcgacgcat cggttggtga
    11521 aagaagtggc ggcgaaagtc ggtctgcctg ccgattggta cgagctttca gaggcaccgg
    11581 ggccgcataa aatgtccaag acccatcgtg aacgttctgt gagcttttta ctgaagcatc
    11641 tcacgggtga atcaaggcag gtcgctgaga ccgagacgac cgagttcacg gcggatgact
    11701 gcactgtaac tcctgacgag gaagtctcgc atctcgttgg ctttaagtcg atttacgata
    11761 cgatcgccga gaagttcgtc gcgcgcggcg tgtcggtcga taacgccgcg cagaacgcaa
    11821 aaccgcttgt ggcgaaagaa ctcatcggta aggattgcag cggtgttctt gcaacgctaa
    11881 aaggagaact ggccaaggat aggcgtgcga ccttgcgttt gggtgggctg gccggtgcgg
    11941 gcgaagtgac ggccacgcta tttgccgacg caggccgcta tgtcatccgt tcggagcgga
    12001 aaggtaaacg atcgtactac gaaaggcgcg gtgacgacga ggtggtggcc gtggatctct
    12061 atatcgcggg acgttctctc gtcgccttgc gcgcagcaga aatcctcacg ctggctgcag
    12121 aactggagaa gcggacgggc gtgaaaccga tgcttgttgc cgaaggccgt tacgttacca
    12181 ttgcgaaatt cgcgctggct gcggatccga cggcattcgt aggggttgaa taccgcaatg
    12241 agccaaaatc atttctcgat tcactcaagg cgcgcgagta tctgtcgttc gccgattccg
    12301 gtgcacttta ttccggaaag gcaggcagat gactcggtat ggagagatga gatgaacaga
    12361 tgtgtgattc cttttgtatg cggcattctt ttcgcgttgt cgatgcgtct ggaggcgtcc
    12421 acgatcacac gggatgtggc ggtcgatccg atcacgacgc gctggacctt cggcgcgcac
    12481 gagcctttca cgatgtaccg ccgcattggc aggaggtcca ccggtggtat ctacggaggc
    12541 tcaaagtggg tgaaagactg gctcgactgg tgggacgagc acgcgccggc gcggatggag
    12601 gagatcggac tcaacgctct ccactcccgc ttctacaaag gcatgggctg ggaggttgag
    12661 aaaaaggatt tcccgaatgt gaagaagttt gtcgataatt gtcataagca cggtgtcacc
    12721 gtacttgctt acgtgcagtt cgcgacactc taccatgagg tgatgcagaa ggagattccg
    12781 gacatcgaaa gctgggcggc gattgatgag cgcggaatga agatgatcta cagtaggcaa
    12841 gcggcccgct ggctcccctg catagtcaat gagaagtggg tcgaatatac ggagaaaatc
    12901 cttacgatcg ccatgaccga aggcggtttc gacggcgtga tgttcgacaa tgccttcttc
    12961 tttggttgcc attgtccgcg gtgccaagag gcgttcagac gtcacattca ttccctgccc
    13021 gatcttgagg aacgtttcgg ctttgccgaa atgccgggac tgcgtattcc gcaagcgttc
    13081 cgccccgaga acggggacga gtatgatccg gttgcccagg agtattaccg ctggcgcgta
    13141 cagtgtgtca atgcggtgat gaaacgcttc taccgccaca tcaagtcgct caagccggat
    13201 gcgattgttt cggccaattc gggatctttc cgtagcaccg ataactacca cgcgcgaagt
    13261 gtcgatgtca tcgacatggc ggatggagga ttggatctcc tgatgatgca gacgctcaac
    13321 ttccctgctt atctcgatga cccgaagttc cccggccagc attatattgt caaccgtcaa
    13381 cgggatctgc gcatggcgcg tgacctcggc aagcagatcg tcgctctcag tgacggtgcc
    13441 gccgggagcg acgccctcga tgagtcgatg tacttgaggc cgcttgtcga agatcttgtt
    13501 tggggcggca ttccgacgga tcgcaccgtc atgtcgcctg tgcgccgcga gggtttcatt
    13561 gacgaagagc gttttgcgat gcgcaaaaaa gtgcttgccg aattcgacgc tttcgtaaag
    13621 gcgaaccgtg ccgcctttac cggcaagccc tatctgccgg tcagggtgtt ctatccgtct
    13681 gctgcctgcg gactcgcggt ctcgatgaat ctggcggttt cgactgtgga cgagatcctg
    13741 ctgcgccacc atatgccgta cggctacgtt atcgtcaagg gtgctgaagc accgtctatc
    13801 ccggctgact gtgaggtgct ggtgttgccg aactcgaagt ggatgagcga tgctcaggtc
    13861 tccgcagtgg tggattgggc gaaaaaaggc ggtaggctcg ttgtcacagg acgcgacacg
    13921 gggctacgcg acgaaaacgg agccgagcgt ttcacgaatc cgcttgattg cgtccgcaag
    13981 ctccccaacg tcgactggcg caacgacatt gacgtcgcgc agaaaaacgc actcggctgg
    14041 atcacgcgcg tgttgccgcc gaaagatggg ggccgggcgt tcatggcggg gcttggtcgg
    14101 gtcggctatc gtaaccgttt tgtgttcgag aacctgccag aggaggtttt cgttgacatg
    14161 cgggaagtca gcgacggtgt tgttatccat ctcgtcaact atcatcccga ggtccagatc
    14221 aaaggtgcga ggctggttgt gccgtcaggc gcgaaagcca cagcagagga gccgttcggc
    14281 acccggcgac aggccgtgcc gctcggtaag gacggtatgc ttcccgcgtt cgggcagtat
    14341 ttgatggtgc ggatttactg aaaacacaag gaggtaaatc aaaatgaaaa aagggacact
    14401 ggctctgctc ggtgcgctga cgctgggttt gaatgtgcgg gcagacacgt ctcccgatcc
    14461 gacgggcgga tatgacatca gcgtcgacta ctcgctcgtg gcgggcctcg tcgagtacac
    14521 caacatctac aatcagaccg cctggacggg tatggacccc ggctatctca cgtctccgtc
    14581 gcagggcgtg acctcgcttt ggatccgtgt ctcgaccgat tcgaatgcga acctgtacta
    14641 tgaggacgga gatagctaca tgaagatgct ctcctactgc gggtacatct ggaataacag
    14701 cccgacgaac gagacgtgga cgttcatatg ctgcatggac gcgaattcca agctgttcat
    14761 cgacggtgaa ctcaagatcg atcagaacac gcgtggatac tgcaagacag gaaatgtggt
    14821 gatgacgccc ggtgcacatg cctttgagtt tcgttatcgc cgcgatggcg ggaattccgg
    14881 cgctatcgct gcgaacatta tgcaatccat cgatgatgag gtcttcacag gctttgccgg
    14941 caatgacggc ggcgagtgga agagcaagtt cggttttgcc atcgatcggc aggggcggag
    15001 atcgcacaac taccgggact acgtgtcgcc ggtcgatccg ggcgatggct cgcttttcac
    15061 ttacacgacg aacctgaacg gtattgcctc gctgtatgtg caacaaggtg ctttccgcta
    15121 tcccgacccc gatgtcgact attcgcgcgt ggcgggcctc ggtgagtaca ccaaccttta
    15181 caatggtgta gcctgggatg cgactgagcc ttgctatctc acgtctccgt cgcagggtgt
    15241 gacctcgctc tgggtgcggg tctcgaccga cgcgaacgcg catctgtact atcaggacgg
    15301 ggacgcggaa aacaagatgc tctcttactg tgggtacgtt tggaatgaca gcccgaccaa
    15361 cgaaacgtgg acgtttgcgt gttgcatgga tgcgcactcc aagctgttca tcgacggtga
    15421 actcaaggtc aatcagaacg tccgtaaaac ctgcaggacg ggaaacgtgg tgatgacgcc
    15481 cggggcgcac gcctttgaat tccgttatcg caaactcggc ggatattctg gtgctatcgc
    15541 tgcgagcatt atgcaatacc tcgatgatga acctttcaca agctacgtcg gcaataacgg
    15601 tggcgagtgg aagagcaagt tcggttttgc catcgaccgg caaggacggg ggtcacacaa
    15661 ctaccgggac tacgtgtcgc cggtcgatcc gggcgatggc tcgctttttg tcgtgacaac
    15721 gaatcgtctg gaggtcagtt cactcgagaa ccggatagac ttcttggcga tgaagtttgc
    15781 gccgaatacg gttttagatc tcaatgcaga gggattgacg acggcagtgt cgtcaattga
    15841 agggcttccg actgttacca acggcaacct gacggtgacg ggtacgtgga cgatccctgc
    15901 tgtcgatatc gccgccggta cggtcttaaa cgccagcgga tcgctgacac tgtccgcagg
    15961 cgcgatgccg attgtgtcaa atgtggcggc tttgccgcgt aacacggtca tcattgcgcg
    16021 tgccgctggc ggactcttcc tgccggaagg catcgacgga ctggcgttgg atccgaaggg
    16081 taagttcgtc ttgtgtcaga cgtcgccgac ggagctcgcg attaagtatt cggtgcaggg
    16141 actgttgatt atgtacaaat aaggagcgcg acgagatgga tcgtatgccg acgaggaaga
    16201 taacgtcgtt cgcattgttc actttgctgg cgacagggtg tgcggttgcc gggacgccgg
    16261 ttgtgataga cgtaccggcc ggaacgaagt tgactgtcat tcgcgatcaa attcgtgctc
    16321 tgagtgttgc ccaacgtgta cagggtgtgg aggtgcggct cgcgcgcggc gtcttcgaga
    16381 tggaggaagg gctcgcgctt gaaccgcagg actctggaac gcccgatgcg ccgatcatct
    16441 ggcggggtgc ggcgtgcgga ggaacgagac ttatcggcgg acgcgcgata ccggcagacg
    16501 tcttcaagcc cgatcccgaa cgcccgggcg ttctggttgc ggacgtttcg gactggcagg
    16561 cgaattggcc ggccaagccg acggagacgg tccatccgta tccggttccg gaactctacg
    16621 tggacggcaa gcggatgatg ccggcgggat ggccggatcc ggtgagtggc gaactggacg
    16681 actgggagga cggctgggcg accttcgata aggtcgtcaa tcccggaacc aacggcgtcg
    16741 actgcggaac cttcgtctac cgggggctcc gtcccgaccg ctgggtgaac gagaagtatg
    16801 cctatgtctt cgggttctgg aagaccgaat ggcgtgccga tgaaatgccg attgcctcga
    16861 tcgacgacaa ggagaagacg attaccctcg gcgggttcca tttttacggc cttggcggaa
    16921 tcaacagttg gagcaatcgg ccccgccgtt ggcgcgtcct caacgtgctc tgcgaactgg
    16981 atactcccgg cgaatgctat gtcgatgtcg aggcgaacaa ggtctacctt ataccgcggc
    17041 atccccttga cgcgcagtcg caagtgcggc tttgctggca ggacgaggac atcgtttcgc
    17101 tacggggtgt tgcgaacgtg acgatctgcg atctgacgat cggccctgca tggaaaaatg
    17161 cggtttgggg ttcggcggtg acgaatgtcg tgatccgcaa cctgacgatt tccggcacgc
    17221 ggctcaaggg cattgagttt tcggaagccg atcattgccg gatcgccgac tgcacgatgt
    17281 atgacttggg cgccgggggc gtgaaggtct cgggcggcga ccgccgcaat ctcatccgcg
    17341 gcgagaatgt ggtggagaac aatctcatcc acgatttctc gaagatccgg atgacgtatg
    17401 cctgggccat ccagacatgg ggcgtcggcg atgtcgtgcg gcacaacgag atgtacaaca
    17461 cgatccacga ggcgttgaac gtggatggaa acgactgcct gatcgaatac aacgtcattt
    17521 ccaatgcggt gaccggcacg gacgattcgg gcgcatttca ttgtggacgc aacatctcct
    17581 gccgcggcaa cgttttccgc cacaatcttt tcgcgaacat cggcaacccc gagttcaggg
    17641 cgcaggcggc tctctatctc gacgacgggc attcgggctg gacgatcgac ggctgtatct
    17701 tccgcgatgt ctttcgtaac gaagacaagc gggggttcgg tgcgatccac tgcaacggcg
    17761 gctattcgaa tgtcgtccgt aattgtctct tcatcaattg tggacgggct ctgggttcgc
    17821 gctgggttac ggacgaaatt tggaataact ggctcaagga gcccggttgg atacgtcggc
    17881 tcaagaggga ggtcgacatc gaaggttccg tctaccagtc gcgttatccg gaactcaaag
    17941 gtgtcatgca gggttttccg cggcgccacc gtatgcagag atgcgaagac aacgtcgtcg
    18001 cgcactgccg cgaatttaag aagggtgaca actgggtcac caatcaatgg aatgccgtgg
    18061 ttaacgaact gccgccggga gatgtcaacg cggcctgccg acagttgatt ccgcatttcc
    18121 agcccatccc ttttgagaaa atcgggcgtt acaggtaagg cggtgttcga tgggaaaaag
    18181 gatcgattcg aacgtgttca ggatgccgac gacggaatcg ggattacgtc gcggtcgagt
    18241 ttagtcgtaa tcgttaattg aaaggaatga acatgagtat aacgagaaga ggtttcatcg
    18301 gtggggtgtc ggcctcgatc tttatcggcg gttgcacgac tgtcaaggga ccgcgtaaga
    18361 tcgcggcgaa cgcgaaagtc aatgtggcga tcatcggctg tggcctcatc gcgaagacga
    18421 cgaacgcgcc gggcttcctg caggatccgc gctgccgcgt gacgtccgtc tgcgatgtcg
    18481 tagagatcgc accggactac ttctacggca gccgcaacac gaacttggga gtcgaggact
    18541 tcgtccgggc ggatggccgt taccgccggg acgtctgcgg ctggcgcgtt ctgcgcgacg
    18601 tggtcaacgc ccattacggt gacaaggcct gcaaggtcgt gttcgactgg cgcacgatca
    18661 tcaacgaccc ggcgattgac gccgtctgca tctgtacgcc ggatcactgg catgcgatca
    18721 tgtccatcgc cgcgatgaag gccggcaagc acgtcttctg ccaaaagccg atgactttgt
    18781 cactggccga gggccagacg atggtacgcg tcgcgaagga gacaggcgtc acctttcagg
    18841 tcggcaatca ggggtcgacg aaccccgcgt accgccttac ccaggaaatt gtccttaacg
    18901 gatacgctgg caaggtgaag agcgcgacga tctgcattcc ggcctgcgac cactgggcgg
    18961 ggcacggccg ttctgcggcg cgactgccgc gtccggccta tctctccgag gcaggttgga
    19021 acatgtggca gggtcctgcc cgtcactggg aggacaacgc ctacatcccc tcgattcacg
    19081 atccgacctg ctggcgtttt aacagccgct atggcggcgg tatgattccc gatttcggtg
    19141 cgcacgaatt cgatcagctc aaccgcggcc tcggcaccca gttcacagga ccggtggcgg
    19201 ttgagaacgt ggagaccgat tacgcgaccg gaacggatcg cgatatcttc tcatggccga
    19261 gcgaattcaa gttcgacttc gttttcgcga acggcgtcag gtgccatgtg cgtaagatcg
    19321 acaaggaggc cggatggccg cgccagacga tcttccactg tgagaagggc gatgttggca
    19381 gctacgccgg aaagggcaag gtgccggaat gcctcaagaa tttcagggag tccgatctca
    19441 ccgacaagga catcaagctt caccgtcccc cggctgagcg cgacggctcg aagtttcacg
    19501 agtctgactt tctggacggt gtcctgaccg gtagtcagga cgtggcctcg acttgcgaga
    19561 tgggacaccg cacgatttcg atggcacaca tcgctaacat ctgcgcgcgg atgcagcttt
    19621 cgtctctcaa atgggatccg aagtccgaga gattcgtcgg cgcctatgcc gacgaggcga
    19681 accgcttcct ctctgccgag tatcacaacg gctgggaatt gggtgcctga gaaacggaaa
    19741 ctttatgaaa agcaggggca tcttggtgtt caacgtgcaa tgctgtttgt ggccggattc
    19801 gtacgctcta caggcgacat ctcacggtta cgctcaatgg tgtcaggatc atggcaacct
    19861 gctgattccc tggggcacct gaggccatcg acgggaacga gttcgtgtct gggccgattc
    19921 tccccaagac gacaattcga atacctcgtt taagaacatg gtgctagcac cgatcgtgaa
    19981 acaaggaagc gaaagaggtc attgatctgg acatgcgcat cgaggtgcag aaacgatctt
    20041 tcaggacaag gtaaatgttt ctttcgtcgc gactacggaa tacgagagaa ggaatgagac
    20101 gatgattgct cttgcggcag gatatcagca tttcgaggat ggcgcaccgt tcgccgagat
    20161 tgccttgcgg catccggcgg tgaaggaggt ctactttccg tgggtggatg agccgtcggg
    20221 acgtcccaaa cttggctacg aggaagagga tgatcctgcg gagcttgagg cagcgctaca
    20281 gcgcgacctt ggccgacttc gtgaagccgg gatgaagctc gatcttcttc tcaacgccaa
    20341 ctgctacgga gagaaggcga tgagccatga gtttgaggag cgcatcatca cgattctcga
    20401 gcagctcata tcgtggcgct gcaagcccga gatcgtgacg accgcctcgc cgtttgttgc
    20461 gcgtacgctt aagaaggtgt tccccgaaat cgagatgcgg gcgagtgtga acatgcggct
    20521 tacgacgcta caggcgttca agtacctcgc gcctcttttc gattcgtact atctcggacg
    20581 cgatgtgcag cgtaacctcg atacggtgag tcggttctcc gattggtgtc atgtgaacgg
    20641 caagaagctc tgcatacttg cgaactccgg gtgcttgagg aactgtccct ggcagacttt
    20701 ccatgacaat ctcattgcgc actccgatgc ggcgatgaat gtggagaacg tgaagggctg
    20761 gagtccacac ctctgctgga cgctttacaa ggatccgaaa aacttccccc atatcctcaa
    20821 ggcgacgtgg gtgcgtccag aagacctcta tcgtttcgaa gggcttgtcg atgtcgtcaa
    20881 actcgcgacg cgccagcatg cgaatccgga tatggtgatc ggtgcctacg agcgcggcgg
    20941 ttttgagggc aatcttctcg accttttcga gccgggcttt tcgcctgcgt tcttcccgac
    21001 tgccctcgat aacgcggcat ttccgcctga ttggtttgac cgcacctctt cctgttcgcg
    21061 tgaatgtgcc gaatgcggat attgcgagca agttcttgaa aaggttaagg tcaacattgg
    21121 atgacggtca tgggaaaatt atcgagtgtc cgcatcgcat gccggacggg agcgaattct
    21181 cggcgctgga tgtgccgctc tgcaaggtca cttggacttc catatttttc ggtgtcatga
    21241 ataacccttg caggccgaca ttgcgccgat ggcggtattt cgggtatagt acgggtgtat
    21301 gcgaatcacg ggcggagaat ggggcggacg gaaactcaag gcaccggcgg gtgaccgtgt
    21361 gcgtccgact caggaccggg tgcgcgaggc gttgttctcc atgctgatgg gggcggtccc
    21421 cggcgcgctc ttcatcgacc tctttgcggg aaccggcgcg gtggggctgg aggcccttag
    21481 ccgcggtgcc agccgtgtgg tttgggtgga gcaggatccg cggtatgcgg ccgtgcttca
    21541 ggagaatgtg gtggccgtga ccggcaagcc cggcgaggtc tgccgttgcg gggtcctccg
    21601 ctggctgaag ggcgcggggt gcgggatgcg ggcggatgtg gtgttcgctg atccgccgta
    21661 cgcagcggac ggtgattcgg gcttcgcgca gatcatgggg ctgctggaga cgaaccgggt
    21721 gttgcggccg ggcgggatat tcgtggcgga gatgccggag gcctgcgcgg cggaggagac
    21781 cgcggggtgg cgcctcgttc gcgaccggac atatgggcac acgcggctgg cggtgtaccg
    21841 gatgggaaca gagggaaccg agacaacgga ttgagcggtt ccggcgtaag ggccggaccg
    21901 tatgagagag aaccttatgg aaaaaattgt agtctatcct ggcacgtttg acccgctgac
    21961 gctgggccac ttcgatctct tgcggcgtag cgcctcgctg ttcgatcagg tgatcgttgt
    22021 ggtcgcggac gtatcggtca aggccggggg catgttcaac ctgcaagaac gcatggccat
    22081 gatccgcgag agcgtcgatg aggtcggcat gaccaacatc gagatcgacc ggttggactg
    22141 tctgttggtt gactattgcc gccgccgcaa ggtcagggtg gtggtgcggg gggtgcgcgt
    22201 gtattcggat ttcgagtacg agttccagat ggcgctgacc aaccgtaaac tcgcgcccga
    22261 gatcgaaacg ctcttcatga tgcccaacga gaactacagt tacgtgacgg cgagcacgat
    22321 tcgcgagatc gcccggtatg gcggggacac gagcgcgttc gtgcccgccc cggtgcagaa
    22381 atacattcgc gcacacatga gcaggcatcc ggatccgcac atccgggcgg gaggcgggat
    22441 tggaaacaca ggggcgtttg atcgttgatg tcgcgcggct cgaggcgggc ggcgaagcgt
    22501 attgcggcga gaccggcacg ggactgctgg agctcggcga gagcgagtgg gtgacgccgg
    22561 tgggcggcat gcgctacgcg ctgcgcgtcg aaatggcggg agacgagctg ctggtgcgcg
    22621 gcgaggtgcg gcagtccttg cgctgcgtct gctcgcggtg cgcggtcgag ttcgacaccg
    22681 aggtggcggt gcccgatttt ttgtgctcgc tggaacggga tacgttggat gagttcgtgg
    22741 acttgaccag tgagcttcgg gaagctacca tacttgccct gcccggttac ccggtttgtc
    22801 gcgagacgtg ccagggcctg tgcatgacgt gcggcgtgaa cctgaacacc gcgtcgtgtt
    22861 catgccggaa acaagaccgg gaccgccgct ggtcggtgct ggatgcgctg tcctgaggat
    22921 ggcggcggaa cgcgggatgg tttttttgaa cgagagaggg aaacatggct gtaccaaaaa
    22981 ggaaaaagtc gaaaatgcgc aagaaccagc gcaaggggca tatcaaagcc aaggtcgcgc
    23041 aggttcaggc gtgcccgagt tgcggcgcgt cgcagcagac ccaccgggtt tgcgcttcgt
    23101 gcgggatgta caagggccgc aaagtgttga cggtcgcgga gtagggcggc cggaaggagc
    23161 ggtatgtgcc cggagaggca tcgaaggggt tttcgcctgg ggcggaaccc ctttgcatga
    23221 tgcgaacgca aacggggtgt gagaatgcgg attgcgttgg atgtgatggg gggggaccgt
    23281 gcgccggagg agatcgtgca tggtgcggtc gaggcgtccc tcaagttcaa agatgtttcg
    23341 aagatcctga tggtcggtga cgaggccgcc atccgggccg aactcgcgaa gtaccccaag
    23401 gcggacctaa cccggttgga catcgtccat gctccggatt cgatcagcat gggggagtcg
    23461 cctgtggtgg cggtgcgcaa aaaaaagcgg tgttccatca acctctgcat ggatttgatc
    23521 aaaaaccgcg aggccgacgc catggtctcg gccggcaact ccggcgcggt ggccgcggcc
    23581 gcgatgttct gcctggggcg gatgcccggc gtgctccgcc cggcgatcgc cacggtgatc
    23641 ccgacgcgcc agcccaagcg gccgctgttg ttgctggacg cgggggccaa cacggattgc
    23701 cacgaagctt ggctcgcgca gttcgccgtg atgggtaacg cttattcgca agcggttctg
    23761 aagcgtaccg agccggtggt ggggttgctc agcatcggca cggaggattg caaggggaac
    23821 gacatgacga agaaagcgtt ccacctgatc caacgctcga aggtgaagtt ccgcggcaat
    23881 gtcgaggggc atgatctctt cctcggcaaa atcgatgtgg tggtgtgcga cggtttcgtg
    23941 gggaatgtgg tgctcaaaac cacggagagc gtggcgcatg cggtcggata ctggatgaag
    24001 agggagtttt tccgccatcc ggtgcgtttg ctgggcgcgc tgctgctgaa gggcgccttg
    24061 gaaacgctga agcgccgcat ggacccggag atttacgggg gcgcgccgtt gctgggggtt
    24121 ccgggcaccg tgatcatcac gcacgggtcc tcttcctacc gcgcgatttt ccacgcgatc
    24181 atcgcgggtg ccgccgcagc cgccaacaac ctttcggata cgatcgcccg ccggatcgcc
    24241 gagatggaga tgccggacga gggcgcggga gtgtagcccc tatttttcta tcgcgccgcc
    24301 gcgtctggat gtgctatgct tccgaaatca gttcaaaaac ggcttgaaat tgacattttg
    24361 cgaaaaaatg tcaaaatgcg ccgcctcata cgtctgtaca tgggacaggt gtggcacaga
    24421 acaagcaact gaggagacgc gaatgagtga tcgggtctgc gtgtttgcag gacaaggcgc
    24481 gcaagtgccg ggtatgggaa aggattttgc cagcgatccg gaggggatgg cccttttcga
    24541 gtgcgcaaac cgggtgttgg gatttgattt ggcgacgctc tgtttcgaag gcccggcgga
    24601 ggcgctgacg aaatccaatg tctgccagcc agcgatcttc acggtcagtg tggcggcgtt
    24661 ccgggcattc cagaaacgct gtccccgcgt ttcgttcgca atggcgggcg gtctgagttt
    24721 gggcgagtgg accgcgctgc atgtggcggg ggtgctggag ttcgaggcgg cgctgaccat
    24781 tctggaggcg cgcgggcgtt tcatgcagca ggcttgcgag gagcaggcga gcggcatgat
    24841 cagcatcatg ggcgcgaccg ccgaacaact tcaaacgctg tgcgaccgca cgggactgac
    24901 ggttgccaac atcaactccg atgcgcaggt cgtgctgtcc ggtctcaagg ccggcgtgga
    24961 atctgcggtg gcggtggcgg ccgaattggg cgtgaaggcg attcccctca atgtggcggg
    25021 cgcgttccat tcgccgctga tggcctcggc ccggtccaag ctggcggagg tgttggaggg
    25081 cgtgaccttc cgcgccccct cgatgccggt gctcgccaat gtgaccggag cgccgcacag
    25141 cacggatccg tcggcgatcc gtgacgccat gctccggcag atcacggatt cggtgcgttg
    25201 gttggactgc gtccggcatg ccgttgctgc gggagccggt ggttttgtgg agttcgggcc
    25261 tggcaaggtg ctgtccggcc tgatccggcg catcgacaag ggtgtggaca cgctcaatgt
    25321 ggcggatgtg acgtcgcttg atgccgcggt tgccgcgctc ggggcgtgag gttcgatttt
    25381 tcggaaaagg agacatcatg ggaatgcttg acgggaaagt ggcggtggtg accggcgcgg
    25441 cgcgcggaat cggtcaggcc atcgcccgga ggctggcgca ggagggcgcc gatatcgtcc
    25501 tttgcgattt gcaggcggag tggctcgccg agagcgcggg gatggttcag gactgcggcc
    25561 gtaaggcgct gccgctggca gtggacgtgg gtgacagtga ggctgtaaac gcctgcgtgc
    25621 aggaggcggt caaggagttc ggcaaggtgg atatccttgt caacaacgct ggcatcacca
    25681 aagacacgct tctggtgcgc atgtcggacg cggattggga tgatgtgatc cgcgtgaacc
    25741 tgaagggcac cttcctgttc acacgcgccg tggccaagaa catgatgcgg cagcggagcg
    25801 gggcgatcat caacatcgcc tcgatcagcg ggctcatcgg taccgccggg caggccaact
    25861 atgcggcttc caaggccggt gtgatcgcat tgaccaagtc cacggccaat gagttggccg
    25921 gacgcggggt cagggcgaac gcgatcgcgc cggggttcat ctcgtcgaaa atgacggacg
    25981 cgctgtcgga agacgtgcgc aaacagtact tggcccggat tccgctgggc cgtttcggaa
    26041 ccgtggagga catcgcgcat gcggtggtgt tcctcgcttc cgagcagtcg agctatctca
    26101 ccgggcagac gctgaacgtc aacggcggca tggtgatgta atcggattgg cgcggcatgt
    26161 gccgcaactg tataacaagg gaaaccagat caaggagaga gtacatggca agcaaactgg
    26221 aagaacgggt caaagagatc atcgttgagc aattgggcgt caacgctgag caggtcacac
    26281 cggaagcatc gttcatcgat gatctgggtg cggactcgct ggacaccgtg gaactgatta
    26341 tggcgttcga agaggagttc ggcgcggaaa ttccggacga ggacgcggaa aagctgactt
    26401 cggtcggcaa ggtgttcgag tatctgaagg aaaagggttt cggacaggac tgagtgtctt
    26461 cccgaacgta t
//
LOCUS       DAOSRB010000015        25411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2135_length_25411_cov_8.403534, whole genome shotgun sequence.
ACCESSION   DAOSRB010000015 DAOSRB010000000
VERSION     DAOSRB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 25411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25411
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_25411_cov_8.403534"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..407
                     /locus_tag="PLZ74_01715"
     CDS             117..407
                     /locus_tag="PLZ74_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR97070.1"
                     /translation="MFRSIKEARMQLGITQDAMAKLLGITRNYLALMETNKRNTPNEV
                     LEKANKLVDTIPENQSVTEWKTRALVAEAKLCQLRAAIRNFNKVVENLEGAL"
     gene            404..688
                     /locus_tag="PLZ74_01720"
     CDS             404..688
                     /locus_tag="PLZ74_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97071.1"
                     /translation="MNVIDDEKKFDMKRKMLRLLSEESGHDKKAVREFKLSSCAASAQ
                     YELTQEEVRRSLDALAGDGYATHDYDELNGIVWRITDSGLSAVSDAGKVK"
     gene            736..1029
                     /locus_tag="PLZ74_01725"
     CDS             736..1029
                     /locus_tag="PLZ74_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97072.1"
                     /translation="MRPKKPNWFMAPFSDEEFIEILRAEHGRVTTVRLERLVRLMRFL
                     QQHGAILMPYDEDEVMNALPRDYGIHPARLKNDLLALYKAKLIIGEKHKVIRR"
     gene            1389..1643
                     /locus_tag="PLZ74_01730"
     CDS             1389..1643
                     /locus_tag="PLZ74_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97073.1"
                     /translation="MMGTLILLLGAFISAWLAFVPCKILLEIRRQGAAREAEALRVVA
                     LLVSIDSRLAGSPPAATPAGAPPPADPLQAAIDARAAGRP"
     gene            2438..2830
                     /locus_tag="PLZ74_01735"
     CDS             2438..2830
                     /locus_tag="PLZ74_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97074.1"
                     /translation="MTPLTPPAEIAAWLACATFAVVLFNQLSRAWFTLRGKPTPIEQA
                     AATSAIRERVSALETSVVKHKAEQDRRLDKLEKAQEGLRDLILKENNLLYNRINACAD
                     ACAAINGEIKLIEENSSILLKTVLEGKK"
     gene            2827..3078
                     /locus_tag="PLZ74_01740"
     CDS             2827..3078
                     /locus_tag="PLZ74_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97075.1"
                     /translation="MNDIDPKVSRAIIAVLKHTGRARPLGVRPLTTFVNGELRAPVDA
                     DAVQAHLSDLEKRGYVERRANTFNPSVIEWIITPSGDAV"
     gene            3068..3688
                     /locus_tag="PLZ74_01745"
     CDS             3068..3688
                     /locus_tag="PLZ74_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97076.1"
                     /translation="MPFSKNETVSFLKKKDRDRMTRKPRPDSNLAGITPEQADRLFEM
                     LRTAPYHAAARRVAEEWGIAVSISGLKRWRDREARARARADLRSAVKASEQFDKDLDA
                     RALDERAAHALRAAFWQAITSGDIASVQRLGSLVLDYNADARDTEKLQRLLKAEKDLK
                     EAREENAALAAQAAALRRQLAEAGKAGAADPAAVADELDRHLGVKK"
     gene            3685..5130
                     /locus_tag="PLZ74_01750"
     CDS             3685..5130
                     /locus_tag="PLZ74_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97077.1"
                     /translation="MTAIPPQDTGYFLPYQLAWILDESEYKVAEKSRRVGFTYASSYR
                     MFRKCMNRGGGFVQWVSSRDMLTAQELIRDYLTIWCKAANIVAKGMYGDNVEVIDVEK
                     NIKAFVVEFPNGARLVSLSSSPEAFAGKGGDVFLDEADLHDDSGRLIDMAQPCTMWGG
                     QLEVVSAYRVNGTSGSPFAMLVKSAKGEANPMGVSLHRVTLPDAVAQGLVEKINKTAG
                     KKQTRAEFIAKIRAKCRTQSAYNSQYLCICDDAAGRLITLQMIAPCELPPAELAEIIA
                     RNPSAPRFGGYDVARRLHASAWHEYVQLGVSLYLARREIFRDAEFDAQENWIRARLTD
                     KGQPRISRMAIDATGLGMHMSERLAKAFPGRVDAVNLESGRRTEMCVALRDRFAAARI
                     YIPADDQLRADLNGPEKTLAKNGGLRIVIPAFDSEAGGQSHCDEFVASMLAVSAADEG
                     AACAFPPRPGRGRRAAARRARRRAMRERRSR"
     gene            5130..6656
                     /locus_tag="PLZ74_01755"
     CDS             5130..6656
                     /locus_tag="PLZ74_01755"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HOR97078.1"
                     /translation="MAKALTKARALKQALETTNKWRDANNPLRSLTMPRAVQLMEMAQ
                     RGVMAELQWLYAAETGIEATDPDLMTIIERTVAGVADCDWHIETNAEDSAGYDAPLAE
                     EQAAFLRESFARCDNLPEAIEHLVMARFRGFSHLSPWLAPDWMIEHLEPLPQWNMVRN
                     GTRNEWAFNPRADNTGYDSLKSGRLTPDDYILLDNRRPVNRIALIKYVRSTVAEKDWD
                     GYVEIYGIPGVFIIMPQNIPDGKEEEYADLAEEATEAGSGALPSGSEVKFSSEARGSQ
                     PFQARLEWLQKQLVLAGTGGLLTMLTEAGSGTLAGSAHQSAWEQIVRRVARVVCEPLN
                     RQYARRALAAAFPGRPALASFALRANQEKDVGDVISNIAQLTSAGFQIDLEQVRRETG
                     YEITGFSAPAPPPGLPAAFRAAARRRPAGRAHDPLMQSGADALAQAQAVANRPLALAI
                     ARVLTAKDLPAMQAAARRLRGELPQIAAAMLSDPAQAEAYRRIMSSAFLQGLIKEPAQ
                     "
     gene            6653..7687
                     /locus_tag="PLZ74_01760"
     CDS             6653..7687
                     /locus_tag="PLZ74_01760"
                     /inference="COORDINATES: protein motif:HMM:NF021627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HOR97079.1"
                     /translation="MRFANRMFCLARAVLDAPSGARWFHTVPPMGEYPGGNIFRGGQE
                     IKNAVLVIDREAYDRILAAFAADTARLGHGLLVDREHFSLDTDKPSDAMAWATDIRED
                     ADGLWTRWDFTPPGLKMWEDRVLISRSPVMQLEPIGGNRFRPVRLESVAMTNTPHFDL
                     STIAAARAADNQPDGCPPDGNHTQGDNTMNRLLALLGLPETATEDEAVAALQALIDAK
                     SAADQAQADAEAARDEAQAACRKARCDAFIAAHRDAIADEAKFRAAYEANPDATEAAF
                     GAFKAAKPAAPATRISARLAATPGDGAAAAPVTLAAYRAMPPGRDKDAYLARHKDTLL
                     RLEREEAQGN"
     gene            7732..8607
                     /locus_tag="PLZ74_01765"
     CDS             7732..8607
                     /locus_tag="PLZ74_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97080.1"
                     /translation="MNTLTNTALQIYSTKLIMALKRRLAPLFAFSLDLSDDAAKIGDT
                     IKVGLISAATAQDFNATTNNYKASQSDILSKEVKIAKRKLSKFGIDDAQAANYPASWW
                     ERKSEMAVNACASAALNDVWALITAENYGDEETDKLALTLAGFKKAGVGQIRAAAVRK
                     GLTPSASALLLNPEFYSALLADLDSSTYGGEEAIRQGYIPNLLGFGKIIEAPDLEAPG
                     FVCHADAIAFANRYLAPVDPASYSEAGSVTDDDSGLTVGIRKYGDPDTGLYSVSAELC
                     YGLDVGASDALLRLV"
     gene            8661..9026
                     /locus_tag="PLZ74_01770"
     CDS             8661..9026
                     /locus_tag="PLZ74_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97081.1"
                     /translation="MRKMLTIAAAAATAAAGIALAANYSAVSLPAPGVWNPAGIGVAK
                     VAAIQIEGAHPANGTVIISRISKDGAATNALLTRTCSSGAFSGDIGAGTNIYLFAGDR
                     LLRSGTATNECSVMLILNN"
     gene            9119..9568
                     /locus_tag="PLZ74_01775"
     CDS             9119..9568
                     /locus_tag="PLZ74_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HOR97082.1"
                     /translation="MWRQPTTDDFRDAILEDELLAWQEASVAEGKDPAAKAIENAVGV
                     FRAALRSGYTGVIGPAGTLPADLIPNAMHIAVYYFLAGRGGAAVSDGRAQLYRDALDM
                     SRRIADGRLAYTDPDDAEQDAAPSGSFPKPAFRPKRRLLDRQSQSGL"
     gene            9573..10130
                     /locus_tag="PLZ74_01780"
     CDS             9573..10130
                     /locus_tag="PLZ74_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97083.1"
                     /translation="MQLDPSKIWLSGAPPPAMPAAADIAEHGSLVAFQIHVRDVLRAF
                     LPLAPVTAQPKPGACTYADKRTVASEVESALRAIGISMVVALDAGTRKSAAPHAVCLD
                     PFTFAVAIAENPVTNRGASGTGVTASRAAELAMICLSGLPLGNGVCAVQSYTLGGEEG
                     QLQTAEVTFQTAYTITPPASMLAEE"
     gene            10141..11133
                     /locus_tag="PLZ74_01785"
     CDS             10141..11133
                     /locus_tag="PLZ74_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97084.1"
                     /translation="MPPYTPTIDRTTLLEGPAHIIFDKTGAKAAWKYCWCTGSVTVQL
                     VHRPKEMNVSGFGQIDDPRADELIEIDFTPAGNFSEALFDWMFSGVFSLWPGESIFPP
                     ADSPVYVHTLTGRILGVANARVTQFPAVRFGAGGPRFEGAAKITGIIGKNTPRTAAGA
                     LFTPPADEAFTAVPLGTEWVHLPCRASWGLAAPLDLVSDDKGWTLRAAHTVSPRTNPD
                     TGTYDFRVETSAVEVTGRPISIGDEDLISAALAGSSRAIGQSSPSGTLTLAEDSPGLT
                     AKLYGARLVSKPAVFGENEPRSGELTWRAFRTPVTVGEATATKLADVDITPAGT"
     gene            11190..11639
                     /locus_tag="PLZ74_01790"
     CDS             11190..11639
                     /locus_tag="PLZ74_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97085.1"
                     /translation="MTITHRHSSGDTVICHGLGHPGEPGYYTGPLGDSDDTDTEWSVD
                     PLRPIGADTAKPLDRGNGLHTWALWVERRFMTDLEAKAHARAMPGLLPRGPAAVEVTG
                     EEDGYKTVYPDTVLQKLATRRNGCSLDIVFHFVSGVPVREAAEPPTP"
     gene            11649..13076
                     /locus_tag="PLZ74_01795"
     CDS             11649..13076
                     /locus_tag="PLZ74_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97086.1"
                     /translation="MRITPPLAAYCLLLTSACALTDPWGVNVQQHTAVQRTYKQGESW
                     TMQVTLRHGLRPLDLTGATAIFYWHTNAAQNVWWDRPAEITAPKAGLVTAQWTPAMDT
                     GAASYPYWIGIWASGSTSPLWRVTGTIRMLPSPGFTPNTLPLPARSIDFSEISFTNAP
                     WATTNDLASALQAASETNALFDARFRPLFNSTSRWDTAYGWGDWHDALETNRVKRLYA
                     ESGNGILGFSGATPEIYEEGVFTNYVVVLSADFSPRPWAGTAPHPFPFSEGDWSGNFD
                     GTRRATIMLWNTANGQLLYWTSPPGEAYPKTLTFDDFFSEGGIGTGTATIYVTTYTRK
                     QLATILTTLSGYERQSGTDIGSAAITLEPNKNQNISFSFGGIGYYSFATGLIYFDWPT
                     RSGRFALREDITDAINNLDIPDPDLSSRVATTNGTAHNLALTGSATLNGAPISTSPTL
                     VRDGVAYRQVWDTNLLTTAWEPIQQ"
     gene            13073..13873
                     /locus_tag="PLZ74_01800"
     CDS             13073..13873
                     /locus_tag="PLZ74_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97087.1"
                     /translation="MKRCCIYILSVSALSVSAQSWYDLTVHNAVFAGTNMVSGEIRPA
                     GALPSLADFNAAKTEISEAMAVASNSWDIALAASNAAARAAADIADATSNGVWEIVFT
                     LSALRGVSAADDIESKTVGFSVETTPTGRLCHAVQWFSTAPPQLPDFDCTYSTNLVAA
                     GFDTIPVSSSWPDQYGAPPDYGQSGGACYRVSALVPLEWGSCFFKLIATGFVLRGNLL
                     PVVGGIAGGASYDITVLDAAGGTNVTLNVRGGFCVATNNPISIMLYEP"
     gene            13870..14928
                     /locus_tag="PLZ74_01805"
     CDS             13870..14928
                     /locus_tag="PLZ74_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97088.1"
                     /translation="MTIQETAQLELAAVLFITVGAALGLLSRWLIRRHRWPAPETLAM
                     AFILALGAAAYALADAEPGKPAPPQVRKWMRFKKDWARWTHAVQAEWAEFCAAVRQGL
                     ISVQGLYGGVIVVTNTMPENASPPKAMRLRANILANPNNQNLKARPTRLTDNGDGTWT
                     VEITASREITDEPALMMYLRRRSDGAVWWVEHTSSSFPETTAPNAYAYTFEMPAGVEG
                     HLAIADEVMLGGPGGLTVEGLALVDLDAGAIYEGISGDFIDADGRLLQVRRGVFMIDN
                     AKAQSAEGPEPEPMMAGGRQAGPEPLLNAKGAAATATPALRGGGTPREAVIVREPEPV
                     KFEPPLKLAMPPLDGGAR"
     gene            14925..15338
                     /locus_tag="PLZ74_01810"
     CDS             14925..15338
                     /locus_tag="PLZ74_01810"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HOR97089.1"
                     /translation="MIQTLCNLYACTVAAVWSGVGCAMESHGDVILCADTAVRVEYGC
                     LSLRAVILSGWVGVACAVMIRRAWPFPAGLALGALMCWLRVFALVGLACMRPPLFHAL
                     HARCGYAAFAAASLCLIFAVCMYVTAQLKKKESQK"
     gene            15335..15922
                     /locus_tag="PLZ74_01815"
     CDS             15335..15922
                     /locus_tag="PLZ74_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOR97090.1"
                     /translation="MKNATIVLFGDSYTAGRIPHSQTDGAFKKCLSNVHGDFAVSGST
                     ALQWSMENECLPSVCLSDADIAVGSLLGNDVFAALADGKVTLDETLRSMAALAIVLSR
                     IAHTKPVLLMLYPNPYMGVPDKHPEAAEGVRRLNAAIGCVADVINASIGNHPVMPLDL
                     GTVLRPEHFDGVDIHPNEAGYQAMAAAVMDVIGRM"
     gene            15987..16838
                     /locus_tag="PLZ74_01820"
     CDS             15987..16838
                     /locus_tag="PLZ74_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97091.1"
                     /translation="MKDATTNTITPRDEQEAKTLSVGARIATVIGIVGTAVSALLAAF
                     GGLDWITAHLPLLLTGLGSLASGGIASYIAIRRMSIDKIAKGTGLIIIAVGLSGCVAI
                     CADSTGEGASVMAFGWGADSAAALSNVAVTGPSTNDSTGVSFDTANSQQKSAQAIQSL
                     ISLGAALAPVLAGGAPAALAPPSASQAAPANSGSVVAVDGDTDADPAGYNGTPGPSGE
                     GIYGRPSCSRCQAYRAAHPDVAVINIDDASNRDDLWAALRLRGFTGANVSLPVLVTAD
                     GYTQAAR"
     gene            16887..17735
                     /locus_tag="PLZ74_01825"
     CDS             16887..17735
                     /locus_tag="PLZ74_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97092.1"
                     /translation="MPSIPSTLLAPTTFAEALQDRAVRDLLPTSLSSAEIMEIDPLIR
                     RRAVFSARVESSRHLTVLDNIIQMILDPEHNPDGTPRQPGQYMTEARARMLLRAHLEQ
                     IGYRPAAGDEGGIKDLRSEQRIRAQLDYPVSFARNYGHAKQMNAPGALDEFPGQRLLP
                     SYADEPRGDAWWGARWRAAGLPGPFGSDFVALKSDPGWKRLSIFGYPYPPFAWGSKRE
                     TEDVDRETCEAYGLIAPGETPAPMDLGESNPLVASLPDGSPDLISAILAQFPNAEFLN
                     GVLTQT"
     gene            17789..18391
                     /locus_tag="PLZ74_01830"
     CDS             17789..18391
                     /locus_tag="PLZ74_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97093.1"
                     /translation="MNVSIKIENSEAVISVAREAAGPVTDGERAAIGAEVARLLQRHL
                     RARDASNAHAYPEGGRRSHFWRKAAEAVSFTHDDKSVTVTVAHQGVALRYAGAPEGIK
                     PVNKKALAIPAHAHAYGRSPLEFNDLRIVVFRAQNKAALVRKPKKREHLGTVMFWLVR
                     KTKPVPGDPTVLPPENVVLGLAARRLALMRSRRRAQKGAP"
     gene            18388..19809
                     /locus_tag="PLZ74_01835"
     CDS             18388..19809
                     /locus_tag="PLZ74_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97094.1"
                     /translation="MTAQELQLKIDTEYDGKGADAAAKDIEKIGKASKKAGEEQDKAG
                     KHAQQFGQSTGRAAEMVGSLTGAMGQSGPAAARMGAGLRVIKALAEGSAGGLAGLATV
                     LVGMGVSAWAAYKRKVEESQKALQGFLQEMTDRKIAEGARQIDAIASGFSRVEKAISA
                     TRAAQSELAAAWDDLNRAGQEVAAMELERREKADLARVAPGDQAGAAAVRARYAGLRE
                     SAALGQRAGDAIRAEQAARDDLAAISGRRANIEQTLGSSVAARDLIAAQLARSTARAD
                     PMNKDEEERKRATQEVAAFTAQLAKLDKQVADLTDALAAARTSEKAAGIRVQAAGLRA
                     TSGIDAASALAAQNTSDAETAAVRERWGQRLGDLRRRASSASSSLSARSASLSAAAEA
                     FDPQRADYPHQGAWNQAKIRDRRMDDQAKGAARLSASAAKLDDQLSKMKPEQLAGVFD
                     SITAQLARLETAVKNAEQRSKRQ"
     gene            19806..22385
                     /locus_tag="PLZ74_01840"
     CDS             19806..22385
                     /locus_tag="PLZ74_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97095.1"
                     /translation="MSAVYEFCVARSSAGAETWYAAEDLGITSIALERVHGGVDKLIL
                     QCGGQNILTDDAIFSFNAIVRMRRVEGGSAAYMFLGRMQPFARRAAGFGAAGTAVING
                     IWDWFEKTVMRQDWTDGANVTASAPRAVLFWSPAGPVSTGAQIERCIAVARAGGCPCA
                     EPAEGDIAAGFTPPSDEQSNISVAQAVQTCLSYHPHSVAWVDYSEREPRVRVAARQSL
                     PSFTVPLAAAQSEAVAITSREDLIPPAVAIAYQKPWSTGERCGMSTVIDHAPVVPGET
                     DAERAARLSQSDVLWATYDLAGMQAVYAEKAQSLKTEAIDWNANKASWSWWARWCPQL
                     NAANITDVGIMNPSTNAALPKLLLAGAVQEWMGVEEAEATFTCDAQIVTGPLVLPAEI
                     RVEKLVVKLKVTDGATKTYKKKELTSYDSGESIPSGLAALLMAEWQQLHYDGEITLAA
                     HDAPVGVGPGMTLNITGGRAEWAAMQAMIVSVAQDLGNGRTTIRFGVPQWIDIDSRVA
                     FYRSCRSRRYTMTRNLSEPQEQGEVEQTDGGDAGIVGLPGSRDGGNVECLIRKVFFNP
                     VAPVPHSVEIDLTDGLTGIAFEDPDDAAIARNIRLKEVLLPVKSGLGDVAVFKKSQIL
                     ACAPYGADVPIRVDPFPGEGLPVQIIPEPPEPIEVTYTEWIGGEPLIDLNSADVQGAP
                     VRIRVVLPGDGNVTPYPCKFIAARFPDKGDEDDSLGIVRGLATDQTHPLKARLAGVDD
                     IPESSTAQRVYGYASPGEWGLIELESLFKYDAPNKRFYIDLAGYTNFKSLTGDSDTWD
                     FNSDANLAGKNGATFDVVTDVSWDSTNNKLVIKKRSITIFKFGLKSIAAAAPTTVVEG
                     EEC"
     gene            complement(23129..23458)
                     /locus_tag="PLZ74_01845"
     CDS             complement(23129..23458)
                     /locus_tag="PLZ74_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97096.1"
                     /translation="MKLHTHVFGGMVLLACSLHLFAKVENDSIQRVDAKNVQVICLND
                     ERKRPFAELWDRYKASSLDEYRQLLATLMTEREKTEVTDSIDREICASFEAEPSSKYY
                     ARRYDTE"
     gene            complement(23711..24529)
                     /locus_tag="PLZ74_01850"
     CDS             complement(23711..24529)
                     /locus_tag="PLZ74_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97097.1"
                     /translation="MKSRITLLLCLISLVCFCCLSPFVQAGDKDVLVGFGTVGDTQAV
                     RVVNINQMKTNSFANLWCQYRVNRDWVVLRIMIGRSSCEITEQTDQEICDLFNSAPTG
                     QLALLLGMCNSGRSRDLLRQHTEATDTELAEACRLGLARRGDKAAEAFFIEKYVEQSR
                     GQAKGTDRNQTDALIESICRLEYIGSVDAILAVFDSVGSDSISSADGVTVKRDTAANM
                     RAFLAQVGAPVPPASSEKELKQWWDENRDAVGKILRDKKDLPRMKMSRVIKSSR"
     gene            complement(24547..>25411)
                     /locus_tag="PLZ74_01855"
     CDS             complement(24547..>25411)
                     /locus_tag="PLZ74_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97098.1"
                     /translation="GGPNNLTVGKEGRIIVPKVEFEEDPNQNYGFDPDGPWVSVEKAK
                     TTTVKVNISPSSAASQIYFTSSDASRVTVSPSRASGPPQILTLTAGDLEGDSAVIEAR
                     IGSAMGQSCTNLGVAVYEKTVVATYVHAVNGFTGIMPFTEANNILKQAVVQIDNVGAH
                     NCSHDLSAGYASDVDMGYAEHESLLNNCHMAIGSTHLQDIYMIPTPGKIRLADGQIKM
                     GVHCSDKFPNIGTLHWNIISQDASLRSRYLAHEIFHMWSDHLNLDDNLMHENEMSMGN
                     TLKKSQWDLAH"
BASE COUNT         4952 a         9175 c         7140 g         4144 t
ORIGIN      
        1 cctttctcgt gttaaccgct tttgttacac ttgcggtgtc tgttacggtg tgacggcgcg
       61 aattatgtaa catagtttgt tgactgtcaa caataattgt tacaaaggcg atagcaatgt
      121 ttcgttcgat aaaagaggcg cggatgcagt tgggcattac gcaggatgcg atggctaaat
      181 tgcttgggat tacacgtaat tatttggctc tgatggagac aaataaacgc aacacgccaa
      241 acgaagtttt ggaaaaagcg aacaagcttg ttgacacgat acccgagaac caatctgtaa
      301 cagaatggaa aacacgggcg ttggttgccg aagcaaagct ctgtcagctt agggctgcaa
      361 tccgcaactt caacaaggtg gtagagaatc tggaaggtgc gttatgaacg tcattgatga
      421 cgaaaaaaag ttcgacatga aacgaaagat gctcaggctt ttaagtgaag aatctgggca
      481 tgacaaaaaa gccgtccgcg aattcaagct gtcatcgtgc gccgccagcg cacagtacga
      541 acttacgcag gaagaggtgc gccgctcact cgacgcactt gccggagacg gctatgcaac
      601 gcatgactac gatgagctaa acggtattgt ctggcggata acagacagcg gactttctgc
      661 cgtatcggac gccgggaagg taaaatgacc aagcaccact gacaacgtaa aaattgggaa
      721 actggaggaa gccctatgag accaaaaaaa ccaaactggt ttatggcacc atttagcgac
      781 gaagaattca tagagatcct tagagcagaa cacggccggg taaccacagt cagacttgaa
      841 aggcttgtgc ggctaatgcg ctttcttcaa caacatggtg ccattctaat gccctatgac
      901 gaagatgaag ttatgaatgc cttgccgcgc gactacggaa tacatcccgc gagactgaaa
      961 aacgaccttc tcgcactgta caaagcaaaa ctaatcatcg gggaaaaaca caaagtgatt
     1021 cgtcgttaag tgcgtatttt tttgggttct gacgtttgaa tccaaaaacg aaaccgtttc
     1081 gtttttgaaa acagccccgc gttggcgggg cgcatgaaag ggaaaagcca tgatgacacc
     1141 gaggtgtcca aaatgcgaaa gcatgtcgtt tgagatcggc ccaataaaga ttaagaacgc
     1201 ccggttcgcc cacaacgcga ttgtgtgcgc gatgtgcggg tgcgttgtcg ggatcgagga
     1261 acatctttcg atgatgtaca tgctcagcaa gatcgccgag aagctcggcg tagatttcaa
     1321 cagataacag ttgtcgcacc acacccccgc agatgcgggg ccatgaaagg aaagagaggt
     1381 ctgtgtctat gatgggaacg ctcatcctgc tgctgggggc tttcatttcg gcctggctgg
     1441 ccttcgtgcc ctgcaagatc ctgctggaga tccgcaggca gggcgcggcg cgcgaggcgg
     1501 aggcgctgcg cgtggtcgcc ctcctggtct ccatcgacag ccgcctcgcc gggtctccgc
     1561 ccgccgcgac gcccgccggc gcgcccccgc ccgccgatcc gctgcaggcc gccatcgacg
     1621 cccgcgccgc cggccggccg tgaatccaaa gccgtaaatc caaaaacgaa accgtttcgt
     1681 ttttgaaaac cgccgccccg caggccgcct gcgggcggtc ttttttttgc cccggaacca
     1741 aaaacgaaac cgtttcgttt ttgccgcgcg atccaaaaac gcagccgctt cgtttttgcc
     1801 gccgcccgga ttcacgcctc ccgcctctca ccccttcact tttttgccgc cgtcacatct
     1861 cacctctcac cttttccaaa aacgaaaccg tttcgttttt gaaaaaacgt gcgcacgcac
     1921 ggcacgcagc agacccgccc gaacagagtt cgggcgtatc atgccgccat cgaaagcagc
     1981 gaagccttca cccggcggat gaagggagcg ccacgcaaaa aatgacagcg tcagcacagg
     2041 catacttgaa tactttccaa aaacgaaacc gtttcgtttt tgaatccgcg cgccgcaacc
     2101 cgcgcaccgg gcagggcgcc cccgcgcgcc cgcgacgtgt ccctgcggca gcctcccgca
     2161 gcgcccgggc cggcgccccg cgcctgtcgg ccgcgccggc cggacacgct ccgtccccca
     2221 aaaacgaaat gatttcgttt ttgccgcgcg atccaaaaac gcagccgctt cgtttttgcc
     2281 gccgcccgga ttcacgcctc ccgccccccg cctctcaccc cttcactttt ttgccccgga
     2341 accaaaaacg aaatcatttc gtttttgccg ccgtcacctc tcacctctca ccttttccaa
     2401 aaacgaaacc gtttcgtttt tgccggaggc ccgccgcatg acacccctca ccccgcccgc
     2461 agagatcgcc gcgtggctcg cctgcgccac cttcgcggtc gtgctcttca accagctcag
     2521 ccgcgcgtgg ttcaccctgc gcggcaagcc cacgcccatc gagcaggccg ccgcgaccag
     2581 cgcgatccgc gagcgcgtct ccgcgctgga gaccagcgtc gtcaagcaca aggccgagca
     2641 ggaccgccgc ctcgacaagc tggagaaagc gcaggaaggc ctccgcgacc tgatcctgaa
     2701 agaaaacaac ctgctctaca accgcatcaa cgcctgcgcg gacgcctgcg ccgccatcaa
     2761 cggcgaaatc aagctgatcg aagagaactc atcgatcctg ctcaagacag tcctggaggg
     2821 aaagaaatga acgacatcga ccccaaggta tcacgggcca tcatcgccgt gctcaagcat
     2881 acggggcgcg cgcgcccgct cggcgtgcgc ccgctcacca cgttcgtcaa cggcgagctc
     2941 cgcgcgccgg tcgacgccga tgccgtccag gcgcacctca gcgaccttga gaagcgcggc
     3001 tatgtcgagc gccgcgccaa caccttcaac ccgtccgtga tcgagtggat catcaccccg
     3061 tccggagatg ccgtttagca aaaacgaaac cgtttcgttt ttgaaaaaaa aggaccgaga
     3121 ccgcatgacc cgcaagccca ggccagacag caacctcgcc ggcatcacgc cagagcaggc
     3181 cgaccggctc ttcgagatgc tccgcaccgc gccgtaccac gccgcggcca ggcgggtcgc
     3241 cgaagagtgg ggcatcgccg tctccatctc cgggctcaag cgctggcgcg accgcgaagc
     3301 ccgcgcccgc gcccgcgccg acctccgctc cgccgtcaag gccagcgagc agttcgacaa
     3361 ggacctcgac gcccgcgccc tcgacgagcg cgccgcgcac gccctccgcg ccgccttctg
     3421 gcaggccatc acctccggcg acatcgcgag cgtccagcgc ctcggctcgc tcgtcctcga
     3481 ctacaacgcg gacgcccgcg acacagagaa gctccagcgc ctgctcaagg ccgagaagga
     3541 cctcaaagag gcccgcgagg aaaacgccgc gctggccgcg caggccgccg cgctgcggcg
     3601 gcagctcgcg gaggccggca aggccggcgc cgccgaccct gcggccgtag ccgacgagct
     3661 cgaccgccac ctgggggtga agaaatgacc gccatccccc cgcaagacac aggctacttc
     3721 ctgccctacc agctcgcctg gatcctcgac gagtccgaat acaaggtcgc cgagaaatcc
     3781 cgccgcgtgg gcttcaccta cgcctccagc taccgcatgt tccgcaagtg catgaaccgc
     3841 ggcggggggt tcgtccagtg ggtcagcagc cgcgacatgc tcaccgccca ggagctgatc
     3901 cgcgactacc tcaccatctg gtgcaaggcc gccaacatcg tggccaaggg catgtacggc
     3961 gacaacgtcg aagtgatcga cgtcgagaag aacatcaagg cgttcgtcgt cgagttcccc
     4021 aacggcgcgc ggctggtctc cctcagctcg tcgcccgaag ccttcgccgg caagggcggc
     4081 gacgtgttcc tcgacgaggc cgacctccac gacgacagcg gcaggctcat cgacatggcc
     4141 cagccctgca ccatgtgggg cggccagctc gaggtggtct ccgcctaccg cgtcaacggc
     4201 acctccggca gccccttcgc catgctcgtc aagtcggcca agggcgaggc caacccgatg
     4261 ggcgtcagcc tccaccgcgt caccctgccg gacgccgtcg cgcaggggct cgtcgagaag
     4321 atcaacaaga ccgccggcaa aaagcagacc cgcgccgagt tcatcgccaa gatccgcgcc
     4381 aagtgccgca cgcagtccgc ctacaactcg cagtacctct gcatctgcga cgacgccgcc
     4441 ggcaggctca tcaccctcca gatgatcgcc ccgtgcgagc tgccccccgc cgagctggcc
     4501 gagatcatcg ccaggaaccc gtccgcgccg cgcttcggcg gatacgacgt ggcccgcagg
     4561 ctccacgcct ccgcgtggca cgaatacgtg cagctcggcg tctcgctcta cctggcccgc
     4621 cgcgaaatct tccgcgacgc cgagttcgac gcccaggaga actggatccg cgcgcgcctc
     4681 acggacaagg gccagccccg catcagccgc atggccatcg acgccaccgg cctcggcatg
     4741 cacatgtccg agcgcctggc gaaggccttc cccgggcgcg tggacgcggt gaacctcgag
     4801 agcggccggc gcaccgaaat gtgcgtggcc ctgcgcgacc gcttcgccgc cgcccgcatc
     4861 tacatccccg ccgacgacca gctccgcgcc gacctcaacg gccccgagaa gaccctcgcc
     4921 aagaacggcg ggctccgcat cgtcatcccc gccttcgaca gcgaggccgg cgggcagagc
     4981 cactgcgacg agttcgtcgc ctccatgctc gccgtctccg cagccgacga aggcgccgcc
     5041 tgcgccttcc cgccgcgccc cgggcgcggg cgccgcgccg ccgcccgccg cgcccgccgc
     5101 cgcgccatgc gcgaaaggag gtcacgctga tggccaaggc actcacaaag gcccgcgcgc
     5161 tgaaacaggc gctcgaaacc accaacaagt ggcgcgacgc caacaacccc ctgcgcagcc
     5221 tcaccatgcc gcgcgccgtg cagctcatgg agatggcgca gcgcggcgtc atggccgagc
     5281 tccagtggct ctacgccgcc gagaccggca tcgaggccac cgaccccgac ctgatgacca
     5341 tcatcgagcg caccgtcgcc ggcgtcgccg actgcgactg gcacatcgag accaacgccg
     5401 aagactccgc cggctacgac gcccccctgg cggaagagca ggctgccttc ctgcgcgagt
     5461 cattcgcccg ctgcgacaac ctccccgagg ccatcgagca cctcgtcatg gcgcgcttcc
     5521 gcggcttctc ccatttgtcg ccgtggctcg ccccggactg gatgatcgag cacctcgagc
     5581 ccctgccgca gtggaacatg gtgcgcaacg gcacacgcaa cgagtgggcc ttcaacccgc
     5641 gcgccgacaa caccggctac gactcgctca agtcaggccg cctcaccccg gacgattaca
     5701 tcctgctcga caaccgccgc cccgtgaacc gcatcgcgct catcaagtac gtgcgctcca
     5761 cggtcgccga aaaagactgg gacggctacg tcgagatcta cggcatcccc ggcgtcttca
     5821 tcatcatgcc gcagaacatc ccggacggca aagaagaaga gtacgccgac ctggccgaag
     5881 aggcgaccga ggccggcagc ggcgccctcc cgagcgggtc ggaagtaaaa ttctccagcg
     5941 aggcgcgcgg ctcgcagccc ttccaggccc gcctggagtg gctgcagaaa cagctcgtcc
     6001 tcgccggcac cggcgggctg ctcaccatgc tcacagaggc cggcagcggc acgctcgccg
     6061 gctccgcgca ccagagcgcc tgggagcaga tcgtccgccg cgtcgcgcgc gtcgtgtgcg
     6121 agccgctcaa ccgccagtac gcgcgccgcg ccctcgccgc cgccttccca gggcgccccg
     6181 cgctcgcgtc cttcgccctc cgcgccaacc aggagaaaga cgtcggcgac gtgatctcca
     6241 acatcgcgca gctcacctcc gccggattcc agatcgacct cgagcaggtc cgccgcgaga
     6301 ccggctacga gatcaccggc ttctccgcgc ccgcgccgcc gccggggctg cccgccgcct
     6361 tccgcgcagc cgcccgcagg cggccggcag gccgcgccca cgatccgctc atgcagtccg
     6421 gcgccgacgc gctcgcccag gcccaggccg tcgccaaccg cccgctcgcc ctcgccatcg
     6481 cgcgcgtcct caccgccaag gacctccccg ccatgcaggc cgccgcgcgg cgcctccgcg
     6541 gcgagcttcc gcagatcgcc gcggccatgc tgtcagaccc cgcccaggcg gaagcctacc
     6601 gccgcatcat gtcatcagcc ttcctgcaag gcctcatcaa ggagcccgcg caatgagatt
     6661 cgccaaccgc atgttctgcc tagcccgcgc cgttctggac gccccgtccg gggcgcgctg
     6721 gttccacacc gtccccccga tgggcgaata ccccggcggc aacatcttcc gcggcggaca
     6781 agagatcaag aacgccgtgc tcgtgatcga ccgcgaggcc tacgaccgca tcctcgccgc
     6841 cttcgcggcg gacaccgcgc gtctcggcca cggcctcctc gtggaccgcg agcacttctc
     6901 gctcgacacc gacaagccct ccgacgcgat ggcctgggcc accgacatcc gcgaggacgc
     6961 ggacggcctc tggacgcgct gggacttcac gccccccggc ctcaagatgt gggaagaccg
     7021 cgtcctcatc tcacggtccc ccgtcatgca gctcgagccc atcggcggca accgcttccg
     7081 ccccgtgcgc ctcgaaagcg tcgcgatgac caacacaccg cacttcgatc tttcaaccat
     7141 cgccgccgcg cgtgcggcgg acaaccagcc ggacggctgc ccgccggacg gcaaccacac
     7201 acaaggagac aacaccatga acagactgct cgccctgctc ggcctgcccg agaccgcgac
     7261 agaagacgag gccgtcgccg ccctccaggc gctgatcgac gccaagtccg ccgccgacca
     7321 ggcccaggcc gacgccgagg ccgcccgcga cgaagcgcag gccgcgtgcc gcaaggcgcg
     7381 ctgcgacgcc ttcatcgccg cgcaccgcga cgccatcgcc gacgaggcca aattccgcgc
     7441 cgcctacgag gccaaccccg acgcgaccga agccgcgttc ggcgcgttca aggccgccaa
     7501 acccgccgcg cccgcgacgc gcatcagcgc ccgcctcgcc gccacgcccg gcgacggcgc
     7561 cgcagccgcg cccgtcacgc tcgccgccta cagggccatg ccgcccggcc gcgacaaaga
     7621 cgcctacctc gccaggcaca aagacacgct cctccgcctc gagcgcgagg aagcccaagg
     7681 caattgaccg caaaccccgg ccccagcgcc gggacatgaa aggaaacaac tatgaacacc
     7741 ctcaccaaca ccgcgctcca gatctacagc accaagctga tcatggccct gaagcgccgc
     7801 ctcgcgccgc tcttcgcgtt ctcgctcgat ctctccgacg acgccgcgaa aatcggcgac
     7861 accatcaaag tcggcctgat ctccgccgcc accgcgcagg acttcaacgc cacgaccaac
     7921 aactacaagg ccagccagtc agacatcctg agcaaggagg tcaagatcgc caagcgcaag
     7981 ctgtcgaagt tcggcatcga cgacgcccag gccgccaact accccgcctc gtggtgggag
     8041 cgcaagtcag agatggccgt caacgcatgc gcgtccgccg cgctcaacga cgtgtgggcg
     8101 ctgatcaccg ccgaaaacta cggcgacgaa gagacagaca agctcgcgct cacgctggcc
     8161 gggttcaaga aggccggcgt cggccagatc cgcgccgccg ccgtcaggaa agggctcacg
     8221 cccagcgcct cggccctgct gctgaacccc gagttctact ccgcgctgct ggccgatctc
     8281 gactcgtcca cctacggcgg cgaagaagcc atccgccagg gctacatccc caacctgctc
     8341 ggcttcggca agatcatcga ggccccggac ctggaagcgc ccggattcgt gtgccacgcc
     8401 gacgcgatcg cattcgcgaa ccgctacctc gcgcccgtcg atccggccag ctacagcgag
     8461 gccggctcgg tgacggacga cgactccggc ctcacggtcg gcatccgcaa gtacggcgat
     8521 ccagacaccg gcctctactc ggtcagcgcc gagctgtgct acggcctgga cgtcggcgct
     8581 tcagacgccc tgctgcgcct cgtgtaacaa accccgcgtg gccgggcctc cgcgcccggc
     8641 ccctgaaagg aaaaccaacc atgcgtaaaa tgctcacaat cgccgccgcc gccgccacgg
     8701 cggccgcagg catcgcgctc gccgcgaact actccgcagt cagcctcccg gcgcccggcg
     8761 tgtggaaccc cgccggcatc ggcgtggcca aggtcgccgc catccagatc gaaggcgccc
     8821 accccgccaa cggaaccgtc atcatcagcc gcatctccaa agacggcgcc gccaccaacg
     8881 cgctcctgac gcgcacctgc tcgtcaggag cgttcagcgg cgacatcgga gcaggcacga
     8941 acatctacct ctttgccggc gaccgcctgc tgcgcagcgg caccgccaca aacgagtgtt
     9001 ccgtcatgct gatcctgaac aactaggccg cgcccgcgcc gccgccaatt ccggcggcgc
     9061 gggcccttgc ctctcactta ttacctctca cttcttacct cttacctatc accccaccat
     9121 gtggcgccaa ccgacaaccg acgacttccg cgacgcgatc ctcgaagacg agctgctcgc
     9181 ctggcaggaa gcctccgtcg ccgaaggcaa agacccggcg gccaaggcca tcgaaaacgc
     9241 cgtgggcgtc ttccgcgccg cgctgcgctc cggctacacc ggcgtcatcg gccccgccgg
     9301 gacgctcccc gccgacctca tccccaacgc catgcacatc gcggtctact acttcctcgc
     9361 cgggcgcggc ggcgcggccg tgagcgacgg ccgcgcccag ctctaccgcg acgcgctcga
     9421 catgtcccgc cgcatcgcgg acgggcgcct cgcctacacc gaccccgacg acgccgagca
     9481 ggacgccgcg ccctccggct cattccccaa gcccgccttc cgccccaagc ggcgcctcct
     9541 cgaccgccaa tcccaatcag gactctgaca ccatgcagct cgacccctca aaaatctggc
     9601 tatccggcgc gccgccgccc gccatgcccg ccgccgcaga catcgcggag cacggctcgc
     9661 tcgtcgcctt ccagatccac gtccgcgacg tactgcgcgc cttcctcccg ctcgcgccgg
     9721 tcaccgcgca gcccaagccc ggcgcgtgca cctacgccga caagcgcacc gtcgcctcag
     9781 aagtcgaatc cgcgctgcgc gccatcggca tcagcatggt cgtcgcgctc gacgccggca
     9841 cgcgcaagag cgccgcgccg cacgccgtct gcctcgaccc cttcaccttc gccgtcgcca
     9901 tcgccgagaa ccccgtcacc aaccgcggcg cgtccggcac cggcgtcacg gccagccgcg
     9961 ccgcagagct cgccatgatc tgcctctccg gcctgcccct cggcaacggc gtctgcgccg
    10021 tccagtccta caccctcggc ggcgaggaag gccagctcca gaccgcagag gtcacattcc
    10081 agaccgccta caccatcacc ccgcccgctt ccatgctggc ggaagaataa ggagaacacc
    10141 atgcccccgt acacaccgac catcgaccgc accaccctcc tcgaaggccc cgcccacatc
    10201 atcttcgaca agaccggcgc caaggccgcc tggaaatact gctggtgcac cggcagcgtc
    10261 accgtgcagc tcgtccaccg ccccaaggag atgaacgtct ccggcttcgg ccagatcgac
    10321 gacccgcgcg cggacgagct gatcgagatc gacttcacgc ccgcaggcaa cttctccgag
    10381 gcgctgttcg actggatgtt ctccggcgtc ttctccctct ggcccggcga gtcgatcttc
    10441 ccccccgccg actcccccgt ctacgtccac acgctgaccg gccgcatcct cggcgtggcc
    10501 aacgcccgcg tcacccagtt ccccgccgtc cgcttcggcg cgggcgggcc gcgcttcgag
    10561 ggcgccgcca agatcaccgg catcatcggc aagaacaccc cccgcaccgc cgcgggcgcg
    10621 ctcttcaccc cgcccgccga cgaagccttc acggccgtcc cgctcggcac cgaatgggtc
    10681 cacctcccct gcagggcgtc gtggggcctc gccgccccgc tcgacctcgt cagcgacgac
    10741 aaaggctgga cgctccgcgc cgcgcacacc gtcagcccgc gcaccaaccc cgacaccggc
    10801 acctacgact tccgcgtcga gacctccgcc gtcgaggtca ccggccgccc catcagcatc
    10861 ggcgacgagg atttgatcag cgccgccctc gccgggtcat cgcgcgccat cgggcagtcc
    10921 agcccctccg gcacgctcac cctggccgaa gacagccccg gcctcaccgc caagctctac
    10981 ggcgcccggc tcgtctccaa gcccgccgtg ttcggcgaga acgagccccg ctccggcgaa
    11041 ctcacctggc gcgccttccg cacgcccgtc acggtcggcg aagccaccgc caccaaactc
    11101 gcagacgtgg acatcacccc tgcaggaacc tgatccctcg ggcaccgatt cacaccaacg
    11161 cactaatgca ctaacgcact aatgaactaa tgacgatcac ccacagacac agctccggcg
    11221 acacggtcat ctgccacggc ctcggccacc ccggcgagcc cggctactac accggcccgc
    11281 tcggcgactc cgacgacacc gacaccgagt ggtccgtcga cccgctgcgc cccatcggcg
    11341 cggacaccgc caagcccctc gaccgcggca acggcctgca cacctgggcg ctctgggtcg
    11401 agcgccgctt catgaccgac ctggaggcca aggcccacgc ccgcgccatg ccggggctgc
    11461 tgccgcgcgg ccccgccgcc gtcgaggtca ccggggaaga agacggctac aaaacagtct
    11521 acccggacac cgtgctgcag aagctcgcca cccgccgcaa cggctgctcg ctcgacatcg
    11581 tgttccactt cgtcagcggc gtccccgtcc gcgaagccgc cgaaccgcca accccatagg
    11641 agccaaccat gcgcatcaca ccaccgctcg ccgcctactg cctgctgctg acctccgcct
    11701 gcgcgctcac agacccgtgg ggcgtcaacg tgcagcagca caccgccgtg cagcgcacat
    11761 acaagcaggg cgaatcgtgg accatgcagg tcacgctgcg ccacggcctc aggccgctcg
    11821 acctcaccgg cgccaccgcc atattctact ggcacaccaa cgccgcgcag aacgtctggt
    11881 gggatcgccc cgccgagatc accgcgccca aggccggcct cgtcaccgcg cagtggacgc
    11941 ccgcgatgga caccggcgcg gcctcgtacc cgtactggat cggcatctgg gcctccggca
    12001 gcacctcccc gctctggcgc gtcaccggca ccatccgcat gctgccctcg cccggcttca
    12061 cgccaaacac cctgccgctg cccgcgcgct ccatcgactt ctcagagatc agcttcacca
    12121 acgccccgtg ggccacaacc aacgaccttg catccgcatt gcaagccgcg tcggaaacaa
    12181 acgccctttt cgacgcgagg ttccgtccgc tcttcaattc cacctcgcgt tgggacaccg
    12241 cgtacggctg gggtgattgg catgacgcgc tcgagaccaa ccgcgtcaaa cgcctctacg
    12301 ccgaatccgg aaacggcatt ctcggattca gcggcgccac gccggagatc tacgaggagg
    12361 gagtgttcac gaattacgtg gtggttctct ccgccgattt cagcccgcgc ccgtgggctg
    12421 gcacggcacc gcatcccttt ccgttcagcg agggcgattg gtccgggaat ttcgacggca
    12481 cgcgccgcgc gacaatcatg ctgtggaaca cggccaacgg ccagttgctg tactggacga
    12541 gcccgcccgg cgaggcctat cccaagacgc taacgttcga cgacttcttc agcgagggcg
    12601 gtatcggcac aggaacggca acgatctacg tgaccaccta cacgcggaaa caactcgcga
    12661 cgattctgac caccctgtcc gggtacgaga gacaatccgg aacggacatc ggcagcgcgg
    12721 ccatcacgct cgagccgaac aaaaaccaga acatttcgtt cagtttcggc gggatcggtt
    12781 actactcgtt cgcgaccgga ctgatatatt tcgattggcc cacgcgttcg gggcggtttg
    12841 cgctgcggga agacatcacc gacgcgatca acaatctgga catccccgat ccggatctct
    12901 caagccgcgt cgcaaccaca aacggaaccg cgcacaatct cgcgctgaca gggtcggcaa
    12961 cactgaacgg agccccaatt tcaacatcac cgacgctggt gcgcgacggt gtcgcataca
    13021 ggcaggtttg ggacacgaac ctgctcacaa ccgcatggga gccgatccaa caatgaaacg
    13081 ctgttgcatt tacatcctgt ccgtatccgc gctctcggtc agcgcccagt cgtggtacga
    13141 cctcacagtc cacaacgccg tgtttgccgg caccaacatg gtctccggcg agatccggcc
    13201 cgccggcgcg ctgccctcgc tggcagactt caatgccgcc aagaccgaga tcagcgaggc
    13261 catggccgtc gcgtccaact cgtgggacat cgccctggcc gcgtccaacg ccgccgccag
    13321 agccgccgcc gacattgctg acgcgaccag caacggcgtg tgggagatcg tgttcacgct
    13381 gagcgcgctg cgcggcgtga gcgcggcgga cgacatcgaa tcgaaaacgg tggggttcag
    13441 cgtcgagacc acaccgaccg gcaggctgtg ccacgccgtc cagtggttca gcaccgcgcc
    13501 gccgcagctg ccggatttcg actgcaccta ctcgaccaat ctcgtcgccg caggcttcga
    13561 caccatcccc gtttccagct catggccgga ccagtacggc gcccccccag attacggcca
    13621 gagcggcggc gcgtgttacc gcgtcagcgc cctggtgcct ttggagtggg gatcatgctt
    13681 tttcaaattg atcgcgaccg gcttcgtttt gcgcggcaat ttgctgcccg tcgtgggcgg
    13741 catagcgggc ggcgcgtcat acgacatcac cgtgctcgat gcggccggcg gcaccaacgt
    13801 cacgctcaac gtgcgcggcg ggttctgcgt cgccaccaac aacccgatca gcatcatgct
    13861 ctacgaacca tgacgatcca agaaacagcc cagctcgaac tcgccgccgt gcttttcatc
    13921 acggtcggcg ccgcgctcgg cctgctgtcg cgctggctga tccggcgcca ccgctggccc
    13981 gcgcccgaga cgctggccat ggcgttcatc ctcgcgctgg gcgccgccgc ctacgcgctc
    14041 gccgatgccg agccgggcaa gcccgcgccg ccgcaggtaa ggaagtggat gcgcttcaag
    14101 aaagattggg caaggtggac gcatgcggta caggccgagt gggcggagtt ttgcgcggca
    14161 gtacggcagg ggctcatcag cgttcagggg ctttatggcg gtgtgatcgt ggtcaccaac
    14221 accatgccgg aaaacgcctc gcccccgaaa gcgatgcggc tccgcgcgaa cattctggcg
    14281 aaccccaaca accagaatct caaagcccgc ccgacccggc tcaccgacaa cggcgacggc
    14341 acatggaccg tggagatcac ggccagccgc gaaatcaccg acgagcccgc gctgatgatg
    14401 tacctgcgcc gccgctcgga cggcgcggtc tggtgggtgg agcacacctc cagcagcttc
    14461 cccgagacaa ccgcgcccaa cgcatacgca tacacgttcg agatgccggc cggcgtggag
    14521 gggcacctcg cgattgccga cgaggtgatg ctcggcggcc ccggcgggct caccgtggaa
    14581 gggctcgcgc tggtcgatct cgacgccggc gcgatctacg aggggatatc cggagatttc
    14641 atagacgcgg acggacgcct gctgcaggtg cgccggggcg ttttcatgat cgacaatgca
    14701 aaggcgcagt ccgccgaagg gccggagccc gagccgatga tggcgggcgg caggcaggcc
    14761 gggccggagc cgctcctgaa tgccaaggga gctgcggcaa ccgcgactcc ggcactgcgc
    14821 ggcggcggca cgccgcgcga agccgtgatc gtgcgcgagc ctgagccggt gaagtttgag
    14881 ccgccgctaa aactggccat gccgccgctc gatggaggcg cgagatgata cagacgctct
    14941 gcaacctcta cgcatgcacc gtcgccgccg tctggtccgg cgtcggctgc gcgatggaat
    15001 cgcacggcga cgtgatcctg tgcgcggaca cggccgtgcg cgtggaatac ggctgcctgt
    15061 cgctgcgcgc ggtcatcttg agcggctggg tgggtgtcgc ctgcgccgtc atgatccgcc
    15121 gcgcgtggcc gttcccggcc ggcctcgcgc tgggcgcgct catgtgctgg ctgcgcgtgt
    15181 tcgcgctggt cggcctggcg tgtatgcggc cgccattgtt ccacgcactg cacgcacggt
    15241 gcggatacgc ggcgttcgca gccgcttccc tgtgcctcat cttcgcggtc tgcatgtatg
    15301 tgaccgcaca gctcaaaaaa aaggagagtc aaaaatgaaa aacgcaacaa tcgttctttt
    15361 cggcgattcc tatacggcgg gccgtatccc tcatagtcag acagacggtg cgttcaaaaa
    15421 gtgcctgagt aacgtgcacg gcgatttcgc cgtgagcgga tcgactgccc tccagtggtc
    15481 gatggaaaac gagtgtttgc cttccgtctg cttgtcggat gccgatatcg ccgtcggctc
    15541 gttactcggt aacgatgttt tcgccgcact cgcggacggc aaggtcacgc tcgatgagac
    15601 gctgcgctcg atggccgcgc tcgcgattgt cctttcccgt atcgcgcata cgaaacctgt
    15661 tctgctgatg ctctacccga atccctatat gggtgttcct gacaaacatc ccgaagccgc
    15721 tgaaggcgtt cgccgtctca acgccgccat cggttgcgtc gcggatgtga tcaacgcgtc
    15781 aatcggcaat caccccgtca tgccgctcga tctcggcacc gtgctgcgcc ccgagcattt
    15841 cgacggagtc gacatccacc ccaacgaggc ggggtatcag gcgatggccg cagccgtcat
    15901 ggatgtgatc gggcgcatgt agttttcact ctcaccgtta aggccgctgt acggccgcaa
    15961 caaaacaaaa caaaaggaga caaaccatga aagacgcgac aaccaacacc atcacacccc
    16021 gcgacgagca agaggccaag acgctttcag tcggcgcgcg catcgccaca gttatcggca
    16081 tcgtcggcac ggccgtgtcc gcgctgcttg ccgccttcgg cggcctcgac tggatcacag
    16141 cacaccttcc cctgcttctc accggcctcg gctcgcttgc ttccggaggc atcgcctctt
    16201 atatcgccat ccgccgcatg agcatcgaca agatcgcaaa aggaaccggc ctcattatta
    16261 ttgctgtcgg cctttccgga tgcgtcgcga tctgcgcgga cagcacggga gagggagcat
    16321 cggtcatggc tttcggctgg ggcgctgatt ccgccgccgc actctcgaac gtcgctgtca
    16381 caggcccctc cactaacgac agcaccggcg tcagtttcga caccgccaac agccagcaga
    16441 agtccgcgca ggcgatccaa tcgctgatct cgctgggcgc ggcgctcgct cctgtcctgg
    16501 caggcggcgc tccggccgct ctcgcgccgc cgtccgcctc tcaggcagcc cccgcaaact
    16561 ccggttctgt ggttgccgtt gacggcgata ccgatgcaga ccctgcaggc tacaacggca
    16621 ctccagggcc gtcaggagaa ggcatttacg gacgcccctc ctgttctcgc tgccaagcat
    16681 accgcgcggc gcaccctgat gtggccgtca tcaacattga tgacgcctcc aaccgtgatg
    16741 acctgtgggc agccctccgc cttcgcggct tcaccggcgc aaatgtttct cttcccgtcc
    16801 tggtcacggc agacggctac actcaagccg cccgctagtt ttaatctcat ttccgcgatc
    16861 acccagtggt cgcggaaaac ccgactatgc cgtccatccc ctccacactc ctggccccca
    16921 cgaccttcgc ggaagcgctt caagaccgcg ccgtgcgcga ccttctgccc acctccctgt
    16981 cgtccgccga gatcatggag atcgacccgc tcatccgccg ccgcgccgtc ttctccgccc
    17041 gcgttgaatc atcgcgccac ctcaccgtgc tcgacaacat catccagatg atcctcgacc
    17101 ccgagcacaa cccggacggc acgccgcgcc agcccggcca gtacatgacc gaggcccgcg
    17161 cccgcatgct cctgcgcgcg cacctcgagc agatcggcta ccggcccgcc gcaggcgacg
    17221 agggtggcat caaagacctg cgcagcgagc agcgcatccg cgcccagctc gactaccccg
    17281 tgtcgttcgc ccgcaactac ggccacgcga agcagatgaa cgcccccggc gcgctcgacg
    17341 agttccccgg ccagcgcctg ctgccgtcgt atgccgacga gccgcgcggc gacgcatggt
    17401 ggggcgcgcg ctggcgcgcc gccggcctgc ccggcccgtt cggcagcgac ttcgtcgcgc
    17461 tcaagtcaga ccccggctgg aaacgcctca gcatcttcgg ctatccctac ccgcccttcg
    17521 cgtggggcag caagcgcgag accgaagacg tcgaccgcga gacctgcgag gcctacggcc
    17581 tgatcgcgcc cggcgagacg cccgcgccga tggacctcgg cgaatccaac ccgctcgtcg
    17641 ccagcctccc ggacggctcc cccgacctca tctccgccat cctcgcccag ttccccaacg
    17701 ccgagttcct gaacggcgtc ctcacgcaaa cctgaccacc tcccacctct tacctattac
    17761 ctattacttc ttacctatta cctcctccat gaacgtctcc atcaaaatcg aaaatagcga
    17821 agccgtgatc tccgttgccc gcgaagccgc aggccccgtc acagacggcg agcgcgccgc
    17881 catcggcgca gaggttgcaa ggctcctgca gcgccacctc cgcgcccgcg acgccagcaa
    17941 cgcgcacgca tacccggaag gcgggcgccg ctcccacttc tggcgcaagg ccgcagaggc
    18001 cgtatccttc acgcacgacg acaagtccgt caccgtcacc gtcgcgcacc agggggtcgc
    18061 cctgcgctac gccggcgcgc ccgaaggcat caagcccgtc aacaaaaaag ccctcgccat
    18121 ccccgcccac gcccacgcct acggccgcag ccccctcgaa ttcaacgacc tccgcatcgt
    18181 cgtcttccgc gcccagaaca aagccgcgct cgtgcgcaag cccaagaagc gcgaacacct
    18241 cggcaccgtc atgttctggc tcgtccgcaa gaccaagccc gtgcccggcg accccaccgt
    18301 gctgccgccc gagaacgtcg tcctcggcct cgccgcccgc cgcctcgccc tcatgcgctc
    18361 gcgccgccgc gcccagaaag gagccccatg accgcccagg aactgcagct aaagatcgac
    18421 accgaatacg acggcaaagg cgccgacgcc gcggccaaag acatcgagaa gatcggcaag
    18481 gcgtccaaga aggccgggga ggagcaggac aaggccggca agcacgccca gcagttcggc
    18541 cagtccaccg gccgcgccgc cgagatggtc ggctcgctca ccggcgcgat gggccagagc
    18601 ggccccgccg ccgcccggat gggcgccggc ctgcgcgtca tcaaagccct cgccgaaggc
    18661 tccgccggcg gcctggcggg cctcgccacc gtcctggtcg gcatgggcgt ttcggcatgg
    18721 gccgcctata agcgaaaggt tgaggagtcg caaaaggcgc tccagggttt cctgcaggag
    18781 atgaccgacc gcaagatcgc cgagggcgcc cggcagatag acgcaatcgc gtcaggattc
    18841 tcacgcgtcg aaaaagccat ctccgcaacg cgtgccgctc agtccgagct tgctgccgca
    18901 tgggacgatc tcaaccgcgc cggtcaggaa gtcgccgcga tggagcttga gcgtcgcgag
    18961 aaggccgacc tcgcccgcgt cgcgccaggt gatcaggccg gtgccgcggc cgtccgcgcc
    19021 cgctatgccg gcctgcgcga aagcgccgcg ctcggccaac gcgccgggga cgcgatccgc
    19081 gccgaacagg ccgcccgcga cgatctcgcc gcaatctccg gccgccgcgc caacatcgag
    19141 cagactctcg gctcatcagt cgccgcccgc gacctgattg ccgcgcaact cgcgcgctcc
    19201 acggctcgcg ccgaccccat gaacaaagac gaagaagaac gcaagcgcgc aacacaagag
    19261 gttgccgcct tcaccgctca gctcgccaag ttagacaagc aggttgccga tctcaccgac
    19321 gcccttgccg ccgcccgcac atccgaaaaa gccgccggca tccgcgtgca ggccgcaggc
    19381 ctgcgcgcca cgagcggcat agacgccgcc tccgcactcg ccgcgcagaa tacctcagac
    19441 gccgaaaccg ccgccgtccg cgaacgctgg gggcagcgcc tcggcgacct gcgccgccgc
    19501 gcatcatccg cgtcatcatc cctgtccgcg agatccgcct cgctctccgc agccgccgaa
    19561 gcgttcgatc cacaacgcgc cgactatccg caccaaggcg cgtggaacca ggccaagatc
    19621 cgcgaccgcc gcatggatga ccaggcgaaa ggcgcggcgc gcctctccgc ctctgccgcc
    19681 aagcttgacg accagctctc aaaaatgaaa cccgagcaac tggcaggcgt cttcgattcg
    19741 atcaccgccc agctcgccag gctagaaacc gccgtcaaga acgccgaaca aaggagcaag
    19801 agacaatgag cgccgtctac gaattctgcg tcgcccggtc cagcgccggc gccgagacat
    19861 ggtacgccgc cgaagacctc ggcatcacct ccatcgcgct cgaacgcgtc cacggcggcg
    19921 tcgacaagct gatcctccag tgcggcgggc agaacatcct cacggacgac gccatctttt
    19981 ccttcaacgc catcgtccgc atgcgccgcg tcgaaggcgg gtcggccgcc tacatgttcc
    20041 tcgggcgcat gcagcccttc gcccgccgcg ccgcggggtt cggggcagcg gggacagccg
    20101 tcatcaacgg catctgggac tggttcgaga agaccgtcat gcgccaggac tggacagacg
    20161 gcgccaacgt cacggcctcc gccccccgcg ccgtgctctt ctggtcgccg gccggcccgg
    20221 tctccaccgg cgcccagatc gaacgctgca tcgccgtcgc gcgcgcgggc ggctgcccgt
    20281 gcgccgagcc ggcggaaggc gacatcgccg ccggcttcac gccgccgtcc gacgagcagt
    20341 ccaacatctc cgtcgcgcag gccgtccaga catgcctgtc ctaccaccct cacagcgtcg
    20401 cctgggtgga ttattcagag cgcgagccgc gcgtccgcgt ggccgcgcgc cagtcactgc
    20461 catcgttcac cgtccccctt gccgccgccc agtcagaggc cgtcgccatc acctcgcgcg
    20521 aagacctcat cccgcccgcc gtcgccatcg cgtatcagaa gccgtggtcg acaggcgagc
    20581 ggtgcggcat gtcgaccgtc atcgaccacg cgccggtggt gcccggcgag acggacgcag
    20641 agcgcgccgc ccgcctgtcg cagtcagacg tactgtgggc cacatacgat ctggccggaa
    20701 tgcaggccgt ctacgccgaa aaggcccagt ccctgaaaac agaagccatc gactggaacg
    20761 ccaacaaagc atcatggtca tggtgggcca gatggtgccc tcaactgaac gccgcaaaca
    20821 tcaccgacgt cgggatcatg aacccgtcca ccaacgccgc gctgccaaaa ctgctgcttg
    20881 ccggcgccgt gcaggaatgg atgggcgtcg aagaagccga ggccaccttc acctgcgacg
    20941 cccagatcgt gacaggcccg ctggtgctgc ccgccgagat ccgcgtcgaa aaactggtcg
    21001 tcaaactcaa ggtcacagac ggcgcaacca agacctacaa aaagaaagag ctgacatcct
    21061 acgattccgg cgaaagcatc ccgtctggcc ttgccgccct cctcatggcc gaatggcagc
    21121 agctccacta cgacggcgag atcacgcttg ccgcccacga cgcgccggtg ggcgtcggcc
    21181 ccggcatgac gctgaacatc acgggcggcc gagccgagtg ggccgccatg caggccatga
    21241 tcgtcagcgt cgcgcaggat ctcggtaacg gccgcacaac gatccggttc ggcgttccgc
    21301 agtggatcga tatagacagc cgcgtagcct tctatcggtc gtgccgatcc cgccgctaca
    21361 ccatgacgcg caacctgtca gagccgcagg aacagggaga ggtcgagcag acagacggcg
    21421 gcgacgccgg cattgtcggg ctccccggca gccgcgacgg cggcaacgtc gagtgcctca
    21481 tccgcaaggt attcttcaac cccgtcgccc ccgttccgca cagcgtcgag atcgatctta
    21541 ctgatggtct cacaggcatc gcgtttgaag accccgacga tgctgctatc gcacggaaca
    21601 tacgcctcaa agaggttctc cttcctgtga aaagcggcct cggcgacgtc gccgtgttca
    21661 aaaaatcgca gatccttgcc tgcgcccctt acggagccga tgtcccgatc cgtgttgatc
    21721 cattcccagg agaggggttg ccggtgcaga ttatcccaga acctccggag cctatagagg
    21781 taacatatac tgagtggatc ggcggcgagc cgctgataga cctcaacagc gccgatgttc
    21841 agggggctcc agtgcgcatc agggtagtgt tgcctggaga cggaaacgtt acgccatacc
    21901 catgcaagtt catcgccgcc cgtttcccgg acaaaggcga tgaggatgat tccctcggca
    21961 tcgtgcgcgg gctggccacc gatcaaaccc acccgctcaa ggcccgcctc gccggcgtcg
    22021 acgacatccc cgagagcagc accgcgcagc gcgtgtacgg ctatgccagc cccggcgaat
    22081 ggggcctgat cgagctggaa tccctcttca agtacgacgc gccaaacaag cgcttctaca
    22141 tcgatctcgc aggctacacc aacttcaagt cgcttaccgg cgattcagac acatgggact
    22201 tcaatagcga cgcaaacctt gccggcaaaa acggcgcgac attcgatgtt gtcaccgacg
    22261 tttcatggga ctcaacaaat aacaagctgg tcatcaaaaa gcgctctatt acgatcttca
    22321 agtttggcct caagagcatt gcggcggcgg caccaactac cgttgtggaa ggggaggagt
    22381 gctaatgaac aactggttca caaagaacgg gaaagttctc accaacaacg gtaaaatcag
    22441 agggtgctgt tgtagcgaac catgcaccga ttgggttgct tctggcggga ctggctattt
    22501 gtgttcatac gtccgatggg accgcacagg tgatgattgc cccagtggaa cgcttttcat
    22561 gaaccttagg tattcacggg attttacgtg gataagtgca tgcaactcat catccatcga
    22621 cttggatgag ccatacggtg aaacaaagtt atatacgata tgggttccag aaggatgtaa
    22681 tgatgtgtgg agcaattacg tgctattagg tgacaaaatg tttatatttc tgcactcatg
    22741 gtcgtgggtg gcggattagt agtgagatga gatatattat taccaagtca aggtgtgctt
    22801 cttgttatgc ccgcagcctc acgaagtgcg ggatcgatcg tctccgtatt ccgtcacgta
    22861 tcggtttggg cgacaccctc gcagactgga ttcatcgatt cttgcgaatc aagccatgcg
    22921 ccgcctgcca gcgccgccgc cgtatcctga atgtcctctt cccctacatt ccgcgtaact
    22981 ccatcgaata ccgagcgtgg aacgactacc tgacgtccgg gatgccacac cttcctgtca
    23041 tctgggactc agagacgggc ctttgcaagc ctcttgcaac ctttccccaa aatccccacc
    23101 ccgctacgcc tcctgtgtca aaccgcagtc attccgtgtc atagcggcgg gcgtaatact
    23161 tgctggatgg ctccgcttcg aaagaagcgc agatctctcg atcaatagag tcggtcacct
    23221 ctgttttttc tcgttctgtc atcaaggttg cgagcaattg cctgtactcg tccaatgaag
    23281 aagccttgta tctgtcccaa agttccgcaa aaggtctctt gcgctcatca tttaaacaaa
    23341 tcacctgaac atttttcgcg tcaacgcgct gtatggagtc attttcgact tttgcaaaca
    23401 gatggagact gcatgcaaga agcaccatgc caccgaaaac atgtgtgtgt aatttcattt
    23461 ttccgtcctt tcctattgat gcgcaatatc ccattgcgcc ttcttcagtt tgtctcccat
    23521 cgaggtctcg tccggatgca tcaggttccc cgtctcgcta atatgcccgg cccatgtatg
    23581 gaagaactca tgagccatgt agcggtatcg aagggatgca tcttcggcta tgatattcca
    23641 gtgaacatca gggttttccc tttcaagggc tgttcccgat taccgccaat gccctgtgac
    23701 ggtcagattc tcaacggctg gacttgataa ctctcgacat cttcattcgt ggcaggtctt
    23761 tcttatcgcg aagtatcttg ccaacagcgt ctcgattctc gtcccaccat tgtttgagtt
    23821 ctttttcact cgaggccgga ggaacaggcg caccgacctg agcaaggaaa gcgcgcatgt
    23881 tggccgccgt gtctctcttc acggttacgc cgtcagcaga ggatattgaa tctgacccca
    23941 cactgtcgaa cactgcaaga atggcatcta cactgccgat gtattcgagc ctgcaaatcg
    24001 attctatgag cgcatctgtc tggtttctgt ctgttccttt tgcctgtcct cttgactgtt
    24061 ctacatactt ctcaatgaaa aaggcttctg ccgccttatc gccccgccgg gcgagtccca
    24121 accgacatgc ctcagcaagt tctgtgtccg ttgcctccgt gtgttgcctg agcaagtctc
    24181 tactgcgacc gctattacac atccctaaca gcaaggccag ttgtcctgtc ggtgccgagt
    24241 tgaaaagatc gcatatctct tggtctgtct gttccgtaat ttcacagctt gaacggccaa
    24301 tcatgatacg caggactacc caatcccgat ttacacgata ttggcaccac aaattggcga
    24361 aggagttcgt cttcatctga ttgatgttga caacccgaac agcctgagtg tcgccaaccg
    24421 tcccgaaacc aaccaaaaca tccttgtccc ctgcttgcac aaacggtgac aggcaacaga
    24481 aacataccag tgatatcagg caaagcaaca gtgtaatacg agacttcatt gtgattcctt
    24541 tcttgtttag tgagccagat cccattgact ctttttcagc gtgttaccca tcgacatttc
    24601 attttcgtgc atcaggttat cgtccaaatt cagatggtca gaccacatgt gaaaaatctc
    24661 gtgcgcaaga taccgacttc gcagtgaagc gtcctgtgag atgatattcc aatgaagcgt
    24721 cccgatgttg ggaaatttgt cggagcaatg aacacccatc ttaatctgtc catcagccaa
    24781 cctaattttg cccggtgtgg gtatcatgta tatgtcttgc aggtgggtac taccaatcgc
    24841 catgtggcaa ttgttcagca agctttcgtg ttccgcatag cccatgtcga catcgcttgc
    24901 ataaccagca gaaaggtcgt ggctacagtt atgggctcca acgttatcaa tttgaacgac
    24961 agcctgtttc agtatattgt tcgcctccgt gaacggcatg atgcctgtga atccgttcac
    25021 ggcatgcacg tatgtggcga ccactgtttt ttcatagacc gccaccccga ggttggtgca
    25081 gctttgtccc atggcactgc caattctcgc ttcgatcaca gcgctgtcgc cttccagatc
    25141 gccagccgtc agggtaagga tttgcggtgg accggaagcc cggctcggcg aaacggtgac
    25201 cctcgacgca tctgaactcg tgaagtagat ctgggaggcc gcgctggacg gagagatgtt
    25261 gaccttgacc gtggttgttt tggccttctc aacggagacc caagggccgt cggggtcaaa
    25321 tccgtaattt tgattcgggt cttcctcgaa ctccaccttc ggcacgatga tcctgccctc
    25381 tttgccgacc gtcaggttgt tgggtccgcc g
//
LOCUS       DAOSRB010000016        23617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2331_length_23617_cov_6.737246, whole genome shotgun sequence.
ACCESSION   DAOSRB010000016 DAOSRB010000000
VERSION     DAOSRB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23617
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_23617_cov_6.737246"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLZ74_01860"
     CDS             <1..595
                     /locus_tag="PLZ74_01860"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOR97099.1"
                     /translation="GTRWEDFLKERVFDPLGMASSSFKPTDKQLKTQVEMYDCVKDAP
                     AKHRLQNEMQQRPYNGDHVFASAGAGLWTTASDQLKFYKMLMNLGVGENGVRILKEET
                     VKEILAKSTRPKGMGGYSLGLTAPEEDKEDSWFGHGGAWGTNCMVNWHRRELKLWAVQ
                     LCGSPRPWDKARTAAADKFFQYAIDNSSADAYTGRVQ"
     gene            1339..4176
                     /locus_tag="PLZ74_01865"
     CDS             1339..4176
                     /locus_tag="PLZ74_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97100.1"
                     /translation="MCKRLCRMVVALSMITVVRADQHKWKSAVSGNWSDLSKWSIASA
                     LPEVTDTIQYGAVGTFTITNDTGGDITLVKIDQSAVPGATKFCVMTLDMQGRTLTLTV
                     SDNSSIFSSGYYQYKNTRDLDPPTCTYELRNGTFDFTAGRLFLGNNGMEADGGIILSG
                     GATLHAYTYHWNDSSRVFIREGSVWNWSKPTADLSMQSGGWHRGFGFLCVTGENSRVE
                     MNGRSVSINNDDSGVYLFDGGALNCQTLTVGTGSHGSNGFVQVRNGMLCAQSFNLGST
                     ALTAYKPTLTLAGETTLVAVTNTGATANFTCYERTGATIAFEIPADGYRTNGVARAPL
                     LLDNLAFNTTRSTSYPNDGPTSLRIACMAWAVAHPNATIPLVSIRKAGGDKADLEALT
                     NGFVFVDVPTSVIEAGNAPVLSIDEGGTTLFITAPESMGTNPTPPEFTVESVSSGANG
                     IRDIFLTISTYGFLSTQATLVELTVATNETFSGAVVTNLATQIEAPTPYSAAFKLPGC
                     LQRQHYWCRLRVVNDNDLDATVPFEFTDEGWEETYVWANAADGHWDTLANWMTGGAAA
                     TELPHGEDMLSSFPVGSYTVTLDSDSEIRSIGFFTAGTLEVPRVYTFDLNGKTLRTTK
                     VTQSVTGFSGTCPYSPAVPMSGQLVFKNGTFDASLSTSLSTFEDAELRVTDGATVYLK
                     LVMNGAARLIVDNGGFFRPQDHITLNANNRTYKTYGLIRVNGAGSKYGSWADLNVGGT
                     LSAVIVENSGLITVGSLNIGGNRNTPTAPCSNCVARADNGTFELSSSLNLGTANAECY
                     RPELRITGTNGFVRATGSGKISSFYENIGATIALEIPAEGFADTNGTARAPIHLNALQ
                     FVARGAGLADFGATTLEITARDWVKANPKQTIKLVELITPNAEALTKLKNAAVFTDTS
                     DATSPTLTVSEDGKVLYATAPVWPGFIMLFY"
     gene            4424..5215
                     /locus_tag="PLZ74_01870"
     CDS             4424..5215
                     /locus_tag="PLZ74_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97101.1"
                     /translation="MTIRQKVAFGLGCIGCAMAGAGIESARRALKVPSVPDMMQVERE
                     VVRLTRTPGQTVVVRDEALASEAEALRTRVAELEQALAARDAAPAAVAVSHLATNAAP
                     ARAGRPERESWEQRMERMRTEEPERYAEMQKRREEFRQQMEQRARERADFLDAVDVAG
                     MNDAQRENHEKLLALVARANELGMQLMQGGGRGEQGEALRREMGETMSALSTLYEMER
                     DYLLEATAKAVGYDGEDVKAFTEHIRHIMENTSPMPGFGRRRGGR"
     gene            complement(5238..6338)
                     /gene="ychF"
                     /locus_tag="PLZ74_01875"
     CDS             complement(5238..6338)
                     /gene="ychF"
                     /locus_tag="PLZ74_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOR97102.1"
                     /translation="MSLGVGIVGLPNVGKSTLFNALTHAQNAEAANYPFCTIEPNKAT
                     VALADPRLDALAQIVHPEQVVKATVDFIDIAGLVRGASKGEGLGNKFLANIRETDAIL
                     QVVRCFADDDVVHVEGEVDPVRDIEIVETELVLADLQSVEKRLDRIARQARADKDLRK
                     EVEAMERLLRHLDAGNPVRTFPREEADPIANTLTELQLITDKPVIYCANVGEIDLAAN
                     SGSVARVRAFAAEKGSDVVVISAKMEAELASLPVEDREAFLASYGLTESGLDQTIRMA
                     YHTLGLASYFTAGPKEVRAWTFRRGWAAPTCAGVIHTDFERGFIRAEVVAFEDYVRLN
                     GEAGAKAAGVMRVEGKTYAVQDGDVMHFLFSV"
     gene            complement(6433..6518)
                     /locus_tag="PLZ74_01880"
     tRNA            complement(6433..6518)
                     /locus_tag="PLZ74_01880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6483..6485),aa:Leu,seq:caa)
     gene            complement(6524..6597)
                     /locus_tag="PLZ74_01885"
     tRNA            complement(6524..6597)
                     /locus_tag="PLZ74_01885"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6563..6565),aa:Gly,seq:tcc)
     gene            6825..7898
                     /locus_tag="PLZ74_01890"
     CDS             6825..7898
                     /locus_tag="PLZ74_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOR97103.1"
                     /translation="MELRKACKYALAVPTSMGIRITPSNGQPVHCGGQFMMQVTSAET
                     NVASVAAYLGLPVKVLTTFVKDSPIARLIQDNLRSRHMDYEGVEVPQGGPWGYRHQIN
                     IADSGFGSRGPRVWNDRAGEVGRTLNVKDFDLERLFGDEGVQIVHLSGLIGALSPETG
                     TFCLELARAAKKHGTRISFDLNHRASFWKGRESELSAIFAEIASIADILVGNEEDFQL
                     CLGVRGPEAGGKDIKAKIDGFKGMIERVRKAYPHAQTYGTTLREVVDTNRHLWGALMC
                     AGDAWHVVEPRDITVLDRIGGGDGFVGGMLYAILKGWEPSKWIQFAWACGALATTMLV
                     DYALPADEEQVWSVWEGNARVQR"
     gene            complement(8051..8707)
                     /locus_tag="PLZ74_01895"
     CDS             complement(8051..8707)
                     /locus_tag="PLZ74_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOR97104.1"
                     /translation="MSDRTRELNLWTSEKKCRKLVENLARRHFTAVYCACAREAAEYI
                     LKQSADAHTVGFGGSLSVADLELTADLTAQGKEILNHGFPDLTPEQRVEIMKRQQTCD
                     LFLSGANAVTLDGCIVNIDGNGNRVAAMIYGPTKVIVVVGRNKIVEGDVSDAIRRIKD
                     KSAPPNAMRLGRQTPCAATGFCSDCDSPQRICHVTTILDSKPTLTDFHVLVVNEDMGL
                     "
     gene            9098..10828
                     /locus_tag="PLZ74_01900"
     CDS             9098..10828
                     /locus_tag="PLZ74_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR97105.1"
                     /translation="MRGRIGNVAAGVLLAAGLAQAGPTVFRRGMDRVSSLDPAMASSL
                     YAARAVQLVYETLLEYDYRARPYRLIPGLATHLPEVQSNGCVYVFTLHPEARFQADPC
                     FGPDGSGPPRGRPVTAEDVVFSLKRLADRRLASPGSWLVEDVIAGMRAFAETSETGSA
                     TDYGRPVSGLRAVDERTVRIELTRPMHQFIWYLAMAYTAVVPHEAVRCYGTDFGSRAV
                     GSGPYRLTDWKRNHQMSYTRDPEWRGWRSGAAAIDASDGTVPFDRVEYRVIDDVSTQW
                     LCFLAGELDFLGEVSRDNWDVVVDAAGGLTEPLRRRGVTLFAMPTLEVAYVGINMEDP
                     VLGKNRALRQALNCAFDRAAWVRFFNNRAVPCDGPVPPGTEGRLETPFPYAYDLDKAR
                     ALLREAGYPDGMDPRTGRRLELNLDLGRTSQDIRESTELMVSFLAQVGIALKPQYHNW
                     PAFLRRLSERRSQMFRIGWVGDYPDAENFLQLFYSRNVSPGPNRTNYVNPEFDRLYEA
                     ACAAEDAAGRNRHWAAAQRLIREDCPWIFLSYAKAYSLCNPSVLYYMPSDFPYGTEKY
                     LRTRAEREGI"
     gene            10876..11346
                     /locus_tag="PLZ74_01905"
     CDS             10876..11346
                     /locus_tag="PLZ74_01905"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOR97106.1"
                     /translation="MVRYCYRGTAQIGRIGVVGTHKAVTRLLFEEDTPPADAEPGECP
                     LLREALRQITAYLEGRLRCFDLPLAPEGTPFMRRVWKRVLEVPYGTTASYGQIARAIG
                     RPGAARAVGMANGANPIPILIPCHRIIGADGTLIGYGGGLSRKARLLEIERSLF"
     gene            11343..11987
                     /locus_tag="PLZ74_01910"
     CDS             11343..11987
                     /locus_tag="PLZ74_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOR97107.1"
                     /translation="MKDNANKTVAAPFSSVVNPMQPETGTAVESDASLLSAFAEGDAA
                     ALDRLVGRYRSALFSWLLGMTANRADAEDLYQEVWVRIIRHAARFHDVSFRAWMWKIA
                     RNLLIDFRRKRKPVVSLDVVSDEEDRPLIEQLADTTAGPVERVERGDLKACVMRAVKL
                     LPEVQREVFLMRVQGDLPFHEIAKTLDIPLNTALGRMHDAMTKLKRMLAEENLR"
     gene            11984..12412
                     /locus_tag="PLZ74_01915"
     CDS             11984..12412
                     /locus_tag="PLZ74_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97108.1"
                     /translation="MKPQNTFDEIAALERDAAALAPQVREAFRWAGGPSATVEEAIRQ
                     EAARVAARARVRWPVWSLRRLQVVAVACLVLLLAAGGLLLTSPQVYRAKAVRQTAHVG
                     TRPAGGASEDAAGFARLLLNIQGLDEDSFVTDGASEALWL"
     gene            complement(12378..12731)
                     /gene="rsfS"
                     /locus_tag="PLZ74_01920"
     CDS             complement(12378..12731)
                     /gene="rsfS"
                     /locus_tag="PLZ74_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOR97109.1"
                     /translation="MTINSEKIALETASALESKKADAIRIFDVRGMSSVTDFYVVATG
                     TSAPHLKALIAETQHRMKSLGVTAYRKSGEPDSGWVVVDYIHVVVHVFSPEARAYYAI
                     EKLWSTAKELPMPHP"
     gene            complement(12728..13354)
                     /gene="nadD"
                     /locus_tag="PLZ74_01925"
     CDS             complement(12728..13354)
                     /gene="nadD"
                     /locus_tag="PLZ74_01925"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOR97110.1"
                     /translation="MACIGILGGTFNPIHLGHLLIGRAAAEAFGLDRVLLLPCCVSPF
                     KIGAADLAPDADRLAMVRLSIQGDPLFQVSTLDLDRGGVSYTIDTVRALRAQYPNDRF
                     AFIIGMDTLCGLSHWHQADALLKLCEVITVERPGMDVRELPGEALSFPQEVRDRLLAN
                     VIRGPLFDVSASEIRRRIAEGKTIRYLVWHEVEAYIRTRGLYRTVKEM"
     gene            complement(13374..14474)
                     /gene="ugpC"
                     /locus_tag="PLZ74_01930"
     CDS             complement(13374..14474)
                     /gene="ugpC"
                     /locus_tag="PLZ74_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOR97111.1"
                     /translation="MAEVTLNKVCKRYDDGGLAVDRFTLTIRDGEFLVLVGPSGCGKS
                     TTLRMIAGLEEITSGTIHIGDRCVNAVPPKDRDVAMVFQNYALYPHMTVFENMAFGLR
                     LRKFPRAEITARVQEAAGILGLDGLLRRLPKQLSGGQRQRVAVGRAIVRQPKVFLFDE
                     PLSNLDAKMRVEMRGELRRLHRRLAATMVYVTHDQTEAMTMGDRIVVMRRGTIQQAAS
                     PMEVYRRPANRFVAGFIGTPPMNVLRGRLVRQPDDTLLFQYAPDARLPLPPVAMSSLA
                     QAADREVLLGFRPEALGADDAAGGVSLRGTVDGVEPMGAETFVQLVAEGGTRLIARVH
                     PDRVLTPGTVADFPLDMRHALFFDAETEHVIG"
     gene            complement(14495..14797)
                     /locus_tag="PLZ74_01935"
     CDS             complement(14495..14797)
                     /locus_tag="PLZ74_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HOR97112.1"
                     /translation="MNSCRPAPRMENRDAAPAAQEPAYPGEFHFRILTETATHAETAL
                     AARIAAYRVTSPLAVSRDSSTGRYRAFCVSVRLEDREEHLRLDAEIKQVPGVRLVL"
     gene            complement(14794..16491)
                     /gene="recN"
                     /locus_tag="PLZ74_01940"
     CDS             complement(14794..16491)
                     /gene="recN"
                     /locus_tag="PLZ74_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOR97113.1"
                     /translation="MLNHLSVKNLAIVEEAAVTFGPGLNIITGETGAGKSVLMGALDL
                     VLGGRADKSAIREGAAEAWVEAGFALADTREVDALLESAGLPPCEEGALLVRRSVSAS
                     GAGRCLVNDSPATAQTLRRLGNLLVDIHGPHDHQSLLDSAFQRDVLDAYGHCVPQREA
                     CAEVWQTLQTLEARLDELQRDAPNIEAETERLRFIAEEITHAQLTEADEEDLVSRHAQ
                     AAHAEQILALGNAVVEGLTDGEPAVFSGLVAVQTQLTELARLLPEAADWLAEARNAAV
                     QIQELGRAVTDCLSRIEADPERLSHLEERMALVQRLKRKYGSSVSEVLATLEKSEARL
                     QDLATRTEQADALRKEIADATQTLTRRAAALSTVRARAAGALARSITQELHDLGFLKS
                     GFDVELSPCPPGPNGADTVSFGFAPNPGEPMRPLKAIASSGEIARVMLAVKAVLALHD
                     RIPVLVFDEIDANIGGEIGRVVGQKMRRVAETHQVISITHLPQSAVYGHRHVVVTKEV
                     VRGRTRMRITTLDDAGRVDEIARMLGGKGLTSVIESHARELLASASIQTTTPAKGVS"
     gene            16745..17497
                     /gene="tpiA"
                     /locus_tag="PLZ74_01945"
     CDS             16745..17497
                     /gene="tpiA"
                     /locus_tag="PLZ74_01945"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOR97114.1"
                     /translation="MRKKIVAGNWKMNKTASEAVALIEEIRKETEGVSAVEIVVCPPF
                     TNLKDAAAACAGTHVMLGAQNVHWEASGAFTGEISGAMLKDLGVQYVIIGHSERRQYF
                     GETDETVNRRVRAALAVGLIPIVCVGETLAERDAGQMESVVVRQTKEGLADLGEDLGR
                     VVIAYEPVWAIGTGRTASPAQAQEVHALIRRTLAEIAGGDVANTIRIQYGGSMKPENA
                     RELMSQPDIDGGLIGGAALKADSFAAIIRAGM"
     gene            17903..19507
                     /locus_tag="PLZ74_01950"
     CDS             17903..19507
                     /locus_tag="PLZ74_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOR97115.1"
                     /translation="MERETHLVEGSAAQMRVPQEALRRRALEEGVAAYVARERLVPPL
                     TAAEMLAHARAATASENLDDAEAVFAAVLLNNAVWADVVAGIPYERRLLLLPLCLARN
                     EVCRAERDGLGLLCAQCGNCPIGLIQAEADRLGYVSLVAEGTTVVTQLLAGGKVDAVI
                     GVGCMDSLRRMFPIMNAHAIPGQGIPLLTDGCQATEADVAWILRVLRASRQGGVSGVT
                     EIEAVADAVRNWFTCEELAAVLGSAETEAERIGREWLLIGGKRWRPLLTAAVSEALGG
                     ARDAIRPAALAVECFHKASLIHDDIEDQDTERYGVPTVHCRYGVPAAINAGDYLIGEG
                     YRLLAEAAFPDDLRARMVTAAARGHRELCLGQGEELAFSRQPAPVSESAVLRIYERKT
                     AAAFEVAVLLGAIAAGADADTCARLSEFSRAVGTAYQIGDDMEDFFSARGRAADLLAM
                     RPTVFLATACTCDNAEVAAALDSVWNADRAGRQRLIDAITQAGLHTRVRLLYEHYRHE
                     TERTIAGIQHTGLQRLLRHVMGRMLR"
     gene            19537..20988
                     /locus_tag="PLZ74_01955"
     CDS             19537..20988
                     /locus_tag="PLZ74_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR97116.1"
                     /translation="MRIRQAIPFCVTCLAAVCAVGTLAAWLARESAVDASLRLATADG
                     SGAEAAAITESDARGTDLTGCFRANREIPRGATTGNWPGFRGAGRDNVARRAGKLLTA
                     WPETGPAVLWQIPVGDGHAMPALANGCLYLLDYDEARGGDCLRCLNADTGQERWEHLY
                     AVKTKRNHGISRTVAATDGRSVVTIGPQGHVVCLDADDGTYRWGFSLSERYGSRVPLW
                     YVGQCPLIDGDRVILAPAASGVLLCALALSDGKTVWETPNPHGYAMSHSSVMILETEG
                     RRHYVYAGIGGIAGVGAEGAERGTLLWHTEAFRPSVVVPSPVPLPDGRFFMTAGYGSG
                     GALFRVSRVSDGWRAETLFKTDRKTFASEQQTPVFLNGLLYTVLPSDGGGERQQLVCM
                     TLEGQRMWASGRDHTFGLGPYVATPEGMLLLLNDAGTLTLARVGRDGFVPLARHALME
                     GRGRDAWGPMVLADNRLYLRDSKRLFCVQVGAP"
     gene            complement(21001..21222)
                     /locus_tag="PLZ74_01960"
     CDS             complement(21001..21222)
                     /locus_tag="PLZ74_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOR97117.1"
                     /translation="MDEQIKAKLEELRGMLQADGGDMELVSITDKTVRLRLRGACGSC
                     PHATMTLKQGIERVLREQIDPEITVERVV"
     gene            21256..22023
                     /locus_tag="PLZ74_01965"
     CDS             21256..22023
                     /locus_tag="PLZ74_01965"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOR97118.1"
                     /translation="MPENTGCVKLRLTGSGGRAKVGFVNHKAETTVLLANGAYPVHPF
                     PVDALRSARRIVCCDGAADRLSQAGLEPAWVIGDLDSLSPAARVRWPGRVVAVSDPES
                     NDLTKAFRFCVSRGWRDLVIVGATGLREDHTLGNLSLLPDFAQEAEVLLLTDTGTFRP
                     MLRSARLRAHPGQQVSIFSFDPRTVITSEGLRYPLRRLRLSRWWQATLNEACGDEFDL
                     IFEGGPLLVFQTYEIRPNRAPGGGDGEMAGQHESNSV"
     gene            22004..22894
                     /locus_tag="PLZ74_01970"
     CDS             22004..22894
                     /locus_tag="PLZ74_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOR97119.1"
                     /translation="MNRILFEPGELCADGTVTLADARAEHILNVLGLGVGGVVRSGVV
                     NGSAGTSRIIGTEGKRIRLQTDHTQPAEPSGFDLLLAAPRPKVMKRLWPQLAALGAGR
                     VVVLNAAKVEKCYFSSQWLDPARIRPLLIEGLAQAGTTMLPEVRVRQRFKPFVEDELG
                     TLFPTPLRLLAHPGPVVGLAASEWPSGTRPLIAVGPEGGWTDYERAMLTERGFRPFSL
                     GPRTLRTDTACVALIAVLAWLRDSSRWSGGADESRVLSGRLGIGQVAGPSCTINAERR
                     TENHETRTLGSLSSEARYAD"
     gene            22842..>23617
                     /gene="rlmB"
                     /locus_tag="PLZ74_01975"
     CDS             22842..>23617
                     /gene="rlmB"
                     /locus_tag="PLZ74_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOR97120.1"
                     /translation="MKRERWVRYPQKRDTPTECRDWIYGRRPVLEVLKAGRRHLYEAV
                     LPPAGRDAPEIAEMRDLLLARGVPFRTLERSELDESCEGGNHQGVAVRTGGYPYISFD
                     QVLDDVKTDTNAVVLILDHIEDPQNVGSLLRTADAAGVTAVLIPKDRAADVTPAAVRA
                     SAGASEHLRVVKVVNLVRAMKELQACEVWITGLDFGPDAKPYTSIDFKGRAGLVVGSE
                     GRGLSRLVRETCDFIASLPMKGRVESLNAGVAGAIALYEVV"
BASE COUNT         4261 a         6839 c         7889 g         4628 t
ORIGIN      
        1 cggaacacgc tgggaggatt tcctcaagga gcgtgtcttt gatccgctcg gtatggcgtc
       61 cagttcgttc aagccgaccg acaagcagct taagacgcag gtggagatgt acgactgcgt
      121 gaaggacgca cccgcgaaac accgcctgca gaacgagatg cagcaacgcc cctacaacgg
      181 cgatcacgtc ttcgcgtcgg cgggcgcggg gctctggacg accgcgagcg accagctcaa
      241 gttctacaag atgctcatga atctcggcgt gggcgagaac ggtgtgcgga tcctcaaaga
      301 ggagaccgtg aaggagatcc tcgcgaaatc gacacgcccg aagggcatgg gcgggtattc
      361 gctcggactc accgctccgg aggaggacaa ggaagactcc tggttcgggc atggcggcgc
      421 gtggggcacg aactgcatgg tcaactggca ccgcagggag ttgaagcttt gggccgtaca
      481 actctgcggc agtccgcgcc cttgggacaa ggctcgcacg gcggcggcgg ataagttctt
      541 ccagtacgcg atcgacaact ccagtgctga cgcctacacc ggccgcgttc agtaaaccta
      601 aaaagagcag ttgaacgttc aacgctcttt ggtttcaggg tttctgggtt gagggtttca
      661 gggtttcagg gtttctgggt tgagggtttc agggtttcat ctaaatcggg gtcgggatcg
      721 ctatcgggat cgggatcggc gacccgcgac attcgacccg cgacgatcga cagggacgcc
      781 cgcgcttccg acaccgcagg tcgctcgtcg cgggtcgaca tataaggtct gacgggagaa
      841 gcgggcgtcc cgcccgctgg tagggacggt tccctgaacc gtccgcggac gcctcgggga
      901 ggcgtcccta ccgattgatt ggcggacgcc ttagttccgc atatgcaccg gtctccaaaa
      961 ctcagtcgat tgcgcttgcg acgaagggct ttgctaaaat tctacccgtt caactgctcc
     1021 tttttgggtt caacgctctt gggtttccgg ttcctgtaga cagaagaccg taggcgggag
     1081 acgggaaaca agcgtcggca acgcggacgt ccccgccctc aggggcaagc agacgcttgc
     1141 cccttgtttt catttcatga cgtttcatgt gtttcatggt tcgagtgttg aaacccgaaa
     1201 cccgaaaccc aaaacccaat tagctgggga ttgactgcgt tcacatccct ttcgtatact
     1261 atacgcaatg ctgattatgc tgtgattgat caagcagagg tgtggtttcc gaacgcaatc
     1321 agaaggggag gggaacgtat gtgtaaaaga ttatgccgca tggtggtcgc tctttcgatg
     1381 atcacggttg tccgtgccga tcagcacaag tggaaatccg cagtgtccgg taactggagc
     1441 gatctttcga aatggagtat tgcaagcgct ttgccggaag tgacagacac gatccagtat
     1501 ggcgcggtcg gcacgttcac catcacgaac gatacgggcg gcgacatcac gctggtgaaa
     1561 atcgaccagt cggcggtgcc gggtgccacg aagttttgcg tcatgacgct cgacatgcag
     1621 ggacgcacgc tgacgctcac cgtgtccgac aacagctcca ttttctcttc tggatattac
     1681 caatataaaa acacgcggga cctcgatccc ccgacgtgca cctacgaatt gcgcaacggc
     1741 acattcgatt tcacggccgg gcgcctgttt ctcggcaaca acgggatgga ggccgacggc
     1801 ggcatcatcc tctcgggcgg cgccacgctc cacgcgtata cgtaccactg gaacgactct
     1861 tcccgcgtgt tcatccggga gggcagcgtt tggaactgga gcaagcccac cgctgacctc
     1921 tctatgcaga gcgggggatg gcaccgtggc ttcgggttcc tctgcgtgac cggggaaaac
     1981 tcccgcgtgg agatgaatgg acggtcggtc tcgatcaaca acgacgattc aggggtctat
     2041 ctgttcgacg gcggcgccct gaactgccag accctcacgg tcggcaccgg ttcgcacgga
     2101 tcgaacggtt tcgttcaggt ccggaacggc atgctctgtg cccagagttt caatctcggc
     2161 agcacggccc tgacggcgta caagccgact cttaccctcg cgggtgagac aacgcttgtt
     2221 gccgtgacga acacgggagc gacagccaac ttcacctgct acgagcgcac cggcgcgacg
     2281 atcgccttcg aaatcccggc ggacggttac cgcacgaacg gcgttgcccg cgcaccgctc
     2341 ctgctcgaca acctcgcctt caacacgaca cgcagcacgt cctatccgaa cgatggcccg
     2401 acttcgctcc ggatcgcctg tatggcgtgg gccgtcgcgc acccgaacgc gacgatcccg
     2461 ctcgtttcca tccgtaaagc gggcggcgac aaagcggatc ttgaggccct cacgaacggc
     2521 ttcgtgttcg tcgatgtgcc gacctcggtc atcgaggccg gaaacgcgcc ggtcctttcg
     2581 atcgacgagg gcggcacgac actttttatc accgcgccgg agtcgatggg tacaaacccg
     2641 acgcccccgg agttcacggt ggaatctgtc agtagcggtg cgaacggcat ccgcgacatc
     2701 ttcctgacaa tcagcacgta cggctttctc tcgactcagg cgacccttgt ggaactcacg
     2761 gttgcgacga atgaaacctt ctcgggggcc gtcgtgacga accttgccac ccaaatcgag
     2821 gcgcccacgc cctattcggc cgcgttcaag cttcccggtt gcttgcagcg ccagcactac
     2881 tggtgccgcc tccgggtggt gaacgacaac gacctcgacg cgacggtgcc gttcgaattc
     2941 acggacgagg gatgggagga gacctacgtg tgggcaaacg ccgcagacgg gcactgggac
     3001 acgctcgcga actggatgac cggtggcgcg gcggctacgg agttgccgca cggggaagac
     3061 atgctcagtt cgttcccggt cggctcctat acggtcacgc tcgattcgga ttccgaaatc
     3121 cgttcgatcg gattcttcac ggcagggacg ctcgaagtgc cgcgggtcta cacgtttgac
     3181 ctgaacggca agacgctgag gacgacgaaa gtgacgcagt cggtgacggg gttctccgga
     3241 acctgtccat attcccctgc ggtgccgatg tccggacagc tcgttttcaa aaacggcacg
     3301 ttcgatgcgt ccctttcgac gagcttgtcg acgttcgagg atgccgaact ccgggtgacg
     3361 gacggcgcca ccgtatacct caagctggtc atgaacggcg cggcccgcct gattgtggac
     3421 aacggcggct tcttccggcc gcaggaccac atcaccctga acgcgaacaa caggacatat
     3481 aagacatacg gtttgatccg cgtcaacggc gcaggctcga aatacggaag ctgggctgat
     3541 ctgaacgtcg gcggcacgct ttcagccgtg atcgttgaaa acagtggcct gatcacggta
     3601 ggcagcctga acatcggcgg caaccgcaat acgcccactg caccctgttc caactgcgtc
     3661 gcgcgtgcgg acaacggcac gttcgagctt tccagctcgc tcaacctcgg aacggccaac
     3721 gccgaatgtt acaggccgga gctgcgtatc accggcacca acggctttgt gcgggcgacc
     3781 ggatcaggca agatctcctc tttttacgag aacatcggcg caacgatcgc tctggagatc
     3841 cctgctgagg ggtttgcgga taccaacggc acggcacgcg cgccgatcca cctgaacgcg
     3901 ctccagttcg tggcccgcgg ggcgggactc gcggacttcg gcgcgacgac gcttgaaatc
     3961 accgcccggg attgggtgaa ggcgaatccg aaacaaacga tcaaactggt cgaactgatc
     4021 acccccaacg cggaggcgct caccaagctg aaaaacgcgg cggtctttac ggatacgagc
     4081 gacgcgacct ctccgacgct cacggtctcc gaggacggca aggtgctcta cgccaccgcg
     4141 ccggtctggc cgggtttcat catgctcttc tactgacgtg cacgccgccc gcggtttggg
     4201 aaggcgacgg gggcggtctt tgtgatgtgc cggtggcaca cgccgcgtcc ttccgctgac
     4261 ggttttcggt gcggggtgtc aacatgtgta cgggcgtgcc gccggggtgt cccgtgacgg
     4321 gatgacgttt ttttggtttg aaccgcaata tcgtgtccgc tgatccgtta gcatgaacca
     4381 tgttgactgc atggcaccgg caagcagaga atggggagag ggaatgacga ttcggcagaa
     4441 agtggcgttc ggattgggat gcatcggatg cgcgatggca ggcgctggaa tcgaatccgc
     4501 acggcgggcg ctcaaagtgc cttcggttcc cgacatgatg caggtggagc gggaagttgt
     4561 tcggctaacc cgcactccgg ggcagacggt ggtggtgcgg gatgaggcgc tggccagtga
     4621 ggctgaagcg ctgcgaactc gggttgccga gttggagcag gcattggccg cacgggatgc
     4681 ggcaccggcc gctgtcgcgg tgtcgcatct tgccacgaat gccgcaccgg cacgtgccgg
     4741 tcggccggaa agggagtcgt gggagcagcg gatggagcgg atgcggactg aggagccgga
     4801 gcggtatgcg gaaatgcaga agcggcgcga ggagttccgc cagcagatgg aacagcgcgc
     4861 ccgcgagcgt gcggactttc tggatgcggt tgacgtggcg ggtatgaatg acgcccaacg
     4921 ggagaatcat gagaaattgt tggcgctggt cgcccgcgcc aatgagttgg gcatgcagct
     4981 gatgcagggc ggcggacgcg gtgagcaggg cgaggcgttg cggagagaga tgggggagac
     5041 catgtcggcc ctctcgacgc tgtatgagat ggagcgtgat tatctgctgg aggccacggc
     5101 caaagcggtg gggtacgatg gggaggatgt gaaggcgttc accgaacaca tccggcacat
     5161 catggagaac acgtccccga tgccgggctt cggacgacgt cgcggaggca ggtgacgggg
     5221 gagcgcaacg cgcccgctca aacgctgaac aggaagtgca tgacgtcgcc gtcctgcacg
     5281 gcatacgttt tgccttccac ccgcatgacg cccgcggctt tggcgccggc ctccccgttc
     5341 aggcgcacgt aatcttcaaa cgcgaccact tcggcgcgga tgaacccgcg ttcgaaatcg
     5401 gtgtggatga cccccgcgca cgttggtgcc gcccaccccc gtcggaacgt ccaggcccga
     5461 acctctttgg ggccggccgt gaaataactg gccaatccga gcgtgtgata agccatacgg
     5521 attgtctgat cgaggccgct ctcggtcagg ccgtatgagg cgagaaacgc ctcgcggtct
     5581 tcaacgggaa ggctggccag ttccgcctcc attttggccg agatgaccac gacatcgctg
     5641 cctttttctg cggcgaacgc ccggacgcgg gcgaccgatc cgctgtttgc cgcgaggtcg
     5701 atctctccca cattggcgca gtagatcacc ggtttgtcgg tgatgagctg aagttcggtc
     5761 agcgtatttg cgatcggatc cgcctcttcc cgcggaaaag tccgcaccgg gtttccagca
     5821 tccaaatggc gcagaagacg ctccatggcc tcaacttctt tgcgcagatc cttgtcggcg
     5881 cgcgcttgcc gcgcgatacg gtccagccgt ttttcgaccg actgcaagtc ggcgaggacc
     5941 aactcggtct ccacgatttc gatatcgcgc acaggatcga cttcaccctc gacatgcacc
     6001 acatcgtcgt cggcaaagca gcgcacgact tgcaggatcg cgtcggtttc ccggatgttc
     6061 gctaaaaact tgttgcccaa gccttcccct ttgctggcgc cgcgcaccag accggcgata
     6121 tcaataaaat ccacggtggc tttgacgacc tgctccggat ggacgatctg cgcgagcgca
     6181 tccagccggg ggtccgcaag cgcgaccgtc gccttgttgg gttcgatcgt gcagaaggga
     6241 tagttggctg cctccgcatt ctgggcgtgg gtcagagcat taaacagggt agacttcccg
     6301 acattgggaa ggccgacgat accgactccg agtgacatgc ggaagattcc ttttcccgaa
     6361 ccggttgtcc ggcgatcctt gctctggcct gtacgacata ggctgtgttg gcctgatcgg
     6421 acatgatacg catggtgcca ggagagggag tcgaaccctc acaccctttc gggcggcaga
     6481 ttttgagtct gctgcgtctg ccatttcgcc atcctggcgg ggatggagcg ggcgaaggga
     6541 atcgaaccct cgtaaccagc ttggaaggct ggggctctgc cattgagcta cgcccgcaaa
     6601 aatgttgtgt tactttaccg atttccgttt gtgcggtcaa tagcgcggct aatttattca
     6661 cagtcgaatg gtttacggca catgcggcgg cttgccttca accacggaga ttgctactat
     6721 aacagggttt tatggtgttc caagtctggg gcactgcggg aaagacaaga ggggcgcaag
     6781 acgcctgccg catgtactga aaacccttta acacggagat gagtatggag ttgcgcaaag
     6841 cgtgcaagta tgcgttggcg gtgccgacca gcatgggcat tcggatcacg ccttcgaacg
     6901 gccagccggt gcattgcggc ggccagttca tgatgcaggt gaccagtgct gagacaaatg
     6961 tggccagcgt ggcagcctat ctggggttgc ctgtcaaggt cctgaccacg tttgtgaaag
     7021 acagtccgat tgcgcggctc atccaggaca acctgcgcag tcggcacatg gattacgagg
     7081 gggtcgaggt gccgcagggc ggaccttggg gttaccgtca ccagatcaac atcgcggaca
     7141 gcgggttcgg ttcgcgcggg ccgcgcgtgt ggaatgaccg tgcgggcgag gtgggccgca
     7201 cgctgaacgt gaaggatttc gatctggagc ggcttttcgg tgacgagggc gtgcagatcg
     7261 tgcatctttc cggcctgatc ggtgcgctgt cgcctgaaac cgggaccttc tgtctcgaac
     7321 tcgcgcgcgc cgcgaaaaaa cacggtacgc gaatctcgtt tgatctgaac caccgcgcct
     7381 ctttctggaa ggggcgcgag tccgaattgt cggcgatctt tgcggagatc gcgagcatcg
     7441 cggatattct ggtcgggaac gaagaggatt tccaactctg cctcggtgtg cgcggaccgg
     7501 aggcgggcgg caaggacatc aaggctaaga tcgacgggtt caaagggatg atcgaacgcg
     7561 tccggaaggc ctatccgcat gcgcagacct acggcacgac gttgcgcgag gtggtggaca
     7621 ccaatcgcca tttgtggggc gccctgatgt gcgcgggcga cgcttggcat gtggtcgagc
     7681 cgcgtgacat cacggtgctg gaccgtatcg gcggcggcga cgggttcgtg ggcggcatgt
     7741 tgtacgcgat tctgaaaggg tgggagcctt cgaagtggat ccagttcgca tgggcctgcg
     7801 gcgccttggc cacgaccatg ctggtcgatt acgcgctgcc tgccgatgag gagcaggtct
     7861 ggagcgtctg ggaaggcaac gcccgggtcc aacggtagcg cgcgccgggc atcgagcgcc
     7921 ggacgataaa ggagcgggca tgctcttcgg gcatgcccgc ttcaccgggg aagaccccgc
     7981 ttggcgcgct gtccgtccgg tatgtcgcgc gcaaagaaac cgtcgcggaa gaaaacctgc
     8041 gcaaaggcgc ttacagcccc atgtcctcgt tgaccaccag aacatggaag tcggtcaggg
     8101 tcggcttgct gtcgaggatg gtggtgacgt gacagatgcg ctggggagaa tcgcaatcgc
     8161 tgcaaaagcc ggtcgccgcg cagggggttt gtctcccgag gcgcatggcg ttcggcggcg
     8221 cggacttgtc cttgatacgc cggatggcgt cactcacatc gccctccaca attttgttgc
     8281 gtcccacgac cacgatgact ttggtcgggc cgtaaatcat agccgcgacc cggttgccgt
     8341 ttccgtcgat attcacgatg cagccgtcga gcgtgaccgc gttcgcgccg gagaggaaca
     8401 ggtcacacgt ctgttggcgt ttcatgatct cgacgcgctg ttccggagtg agatccggga
     8461 acccgtggtt caggatctcc ttgccttgag cggtgaggtc ggcggtcagt tcgaggtcag
     8521 ccacggagag tgatccgccg aatccgacgg tgtgcgcgtc tgcgctctgc ttcagaatat
     8581 actctgcggc ctcacgggcg caggcgcaat atacggcggt gaagtgacgg cgggcaaggt
     8641 tctcgaccaa tttgcggcac tttttttccg aggtccagag attcagttca cgggtacggt
     8701 ctgacatagg tattccttat ttggttttgc gaaacaagac aatcatccgc tgcgggacgc
     8761 ctctgcggat cgtccctgaa acagcggtat tccacatcca aaaacatcat gtggaatttt
     8821 acatgatccc tcatgggatt acaatcctca aagcggtttt cccgcaacgc gcggatcagt
     8881 cgaatcgggg tgtccttaca agagaacgta aacgtcacag gtcgaatgcc gcggggcgca
     8941 ggtcatgttc acgggaatgg ggacggggaa gaggacgagg acgtgtgcgg ggaacgcgtg
     9001 cccgcatctc cttgacaacc gtggggcgtc ccgtaagata catgttcacc gggatcgggg
     9061 agtgtccggt tttcggtgaa aggggcggac gggaggtatg cgcggacgga tcgggaacgt
     9121 ggcggcgggg gtcttgttgg cggcagggct ggcgcaggcg ggaccaacgg tgttccggcg
     9181 agggatggac cgggtctcga gtctggatcc ggccatggcc tcatcgctgt atgcggcgcg
     9241 cgcggtgcag ttggtgtacg agacgttgtt ggagtacgac tatcgcgcgc gtccgtaccg
     9301 cctcattccg gggttggcga cgcatttgcc ggaggtgcaa tccaacgggt gcgtgtatgt
     9361 gttcacgctt catcctgagg cgcgttttca ggcggatccc tgtttcggtc cggacggctc
     9421 cggtccgccg cgcggccgcc cggtgacggc ggaggatgtg gtgttttcgc tcaaacgtct
     9481 cgcggaccgg cgtctcgcct cgccgggttc ctggctggtg gaggatgtga tcgcggggat
     9541 gcgcgccttt gccgagacct ccgagacggg atcggccacg gattacggac ggccggtcag
     9601 cgggttgcgg gcggtggatg agcgcaccgt gcggattgaa ctcacgaggc cgatgcacca
     9661 gttcatctgg tatctggcga tggcttatac tgcggtggtg ccgcacgagg cggtccgttg
     9721 ctacggcacc gatttcggta gccgggccgt gggtagcgga ccgtatcggc tgacagactg
     9781 gaaacgcaat caccagatga gctatacgcg tgatccggag tggcgcggct ggcgcagcgg
     9841 tgccgcagcc atcgacgctt cggacggcac ggtgccgttt gaccgtgtgg agtaccgggt
     9901 cattgacgac gtttccacgc agtggctctg ttttttggcg ggcgagctgg atttcttagg
     9961 tgaggtgtcg cgcgacaact gggacgtggt ggtggatgcg gcaggcggtt tgacagagcc
    10021 gttacggcgg cgcggtgtga ccctcttcgc catgccgacg ctggaggtgg cttatgtcgg
    10081 gatcaacatg gaagatcccg tgctggggaa aaaccgcgcg ttgcgtcagg cgttgaactg
    10141 cgcattcgac cgcgcggcgt gggtgcggtt tttcaataac cgggccgtgc cgtgcgacgg
    10201 gcccgtgccg ccgggcacgg aagggagact ggagacgccg tttccgtacg cctacgattt
    10261 ggacaaggcc cgagcgctgt tgcgcgaggc agggtatccc gacggcatgg atccccggac
    10321 cgggcgacgc ctggagctga atctcgattt gggccgcacc tcgcaggata tccgcgagtc
    10381 caccgaactg atggtttcct ttctggccca ggtgggcatt gccctgaagc cgcaatatca
    10441 caactggccc gcttttttgc gccggttgtc ggagcgccgg tcgcagatgt tccggatcgg
    10501 ctgggtgggg gactatcccg atgccgaaaa ttttcttcag cttttttaca gccggaatgt
    10561 gtcgccgggc cccaatcgga cgaattacgt caaccccgag ttcgaccggc tgtacgaggc
    10621 ggcttgcgcg gcggaggatg cggctggccg caaccgtcat tgggctgcgg cgcaaaggct
    10681 gatccgggag gattgccctt ggatttttct gagctatgcg aaagcctaca gcctttgcaa
    10741 cccgagtgtg ctatactata tgccatctga tttcccgtac ggcaccgaga aatatctccg
    10801 cacgcgcgca gaacgggagg ggatctgaag gaggggttct tgaccgccat catacgatca
    10861 tgcggaaagc cggaaatggt tcggtactgc tatcgcggga ccgcgcaaat cgggcggatc
    10921 ggcgttgtgg gaacccataa agcggtgacc cgcctgcttt ttgaggaaga cacgccgcca
    10981 gccgacgcgg aaccgggcga gtgtcctttg ttgcgggagg cgttgcgcca gatcaccgca
    11041 tatctcgaag gccggttacg gtgcttcgat ttgccgttgg cgccggaggg cacgcccttc
    11101 atgcgccgcg tctggaagcg ggtgctggag gttccgtatg gcactacagc ctcgtatggg
    11161 cagatcgccc gggcgatcgg ccgtcccgga gcggcgcgtg ccgtggggat ggccaatggc
    11221 gccaatccga ttccgatcct gattccctgt caccggatca tcggtgccga cggtacgctg
    11281 atcggatacg gcgggggatt atcgcggaag gcccgtctgt tggagatcga gaggagtctt
    11341 ttttgaaaga caatgccaat aaaacggtag cggcaccgtt ttcttctgtt gtaaacccca
    11401 tgcagccgga gaccgggacc gcagtagaaa gcgacgccag tttgttgagc gcctttgccg
    11461 agggtgatgc ggcggccttg gacaggttgg tggggcggta ccggtcggcg ctgttttcgt
    11521 ggttgctggg catgaccgcg aatcgcgccg acgccgagga tctctatcag gaggtttggg
    11581 tgcggatcat ccgccatgcg gcgcggttcc atgacgtgtc cttcagggcc tggatgtgga
    11641 agatcgcgcg gaatctgctg atcgatttcc gacgcaagcg aaaaccggtc gtgagtctgg
    11701 acgtggtttc ggatgaggag gaccggccgc tgatcgaaca gttggctgac accacagccg
    11761 gaccggtcga gagggtggag cgcggggact tgaaggcgtg tgtgatgcgc gcggtcaagt
    11821 tgttgcccga ggtgcagcgg gaggtgttcc tgatgcgggt gcagggcgat ctgccttttc
    11881 atgagattgc gaagacgctg gacattccgc tcaatacagc actcgggcgg atgcacgacg
    11941 ccatgaccaa actgaaacgg atgttggccg aggagaattt gagatgaagc cgcaaaacac
    12001 attcgacgag attgccgcgc tggagcgtga tgcagcggcg ctcgcaccgc aggttcgcga
    12061 ggcattccgt tgggcggggg ggccctccgc aacagtagag gaggccattc gtcaagaggc
    12121 ggctcgcgtg gccgcgcgtg cccgggtgcg atggcctgta tggtcactgc gccggttgca
    12181 agtcgtcgcc gttgcatgtc tggtgctgct gctggctgcc ggcggtcttt tgttgacttc
    12241 tccgcaggtc taccgggcga aggctgtgcg tcagacggcg catgtgggca cgcggcctgc
    12301 gggcggcgcc tcggaggatg cggcggggtt cgcccggctc ctgctgaaca ttcaggggtt
    12361 ggacgaggat tcgttcgtca cggatggggc atcggaagct ctttggctgt agaccagagc
    12421 ttttcgatgg cgtaatacgc ccgcgcctca ggcgaaaaga cgtgcaccac gacgtggata
    12481 taatccacca ccacccagcc gctgtcgggt tcgccggatt tgcggtaggc agtgaccccc
    12541 aacgacttca tacggtgctg ggtttcggcg atcagtgctt tgagatgcgg ggccgaggtg
    12601 ccggttgcca ccacataaaa atcggtcacg gaggacatgc cgcgcacatc gaagatgcgg
    12661 atcgcgtccg cttttttgga ctcgagtgcc gatgctgttt cgagcgcaat cttttccgag
    12721 ttaatggtca catctccttt actgtccggt aaagtccgcg ggtgcggata taagcttcga
    12781 cctcatgcca aacaagatac cggattgttt tgccttccgc aattcttctt cggatctccg
    12841 aggcggaaac atcgaaaagc ggtccccgga tcacgttcgc cagcagccgg tcccgcacct
    12901 cttgcggaaa ggagagggct tccccgggga gttcccgcac atccatgccc ggccgttcga
    12961 cggtgatgac ctcgcacagc ttgagcagcg catcggcctg atgccagtgg ctcaatccgc
    13021 acagggtgtc catgccgatg atgaaggcga agcggtcatt cggatactgc gcacgcagcg
    13081 cgcgcacggt gtcgatcgta taggagacgc ccccccgatc cagatcgagt gtgctgactt
    13141 ggaacagcgg atcgccctgg atgctcagcc gcaccatcgc cagacggtcg gcgtcggggg
    13201 cgaggtccgc cgcgccgatt ttaaagggcg acacgcaaca cggcagcaac agcacgcggt
    13261 cgagcccgaa ggcctctgcg gccgcgcggc cgatgagcag atgccccaga tggatcgggt
    13321 tgaacgtgcc gcctagaatg ccgatacatg ccatgaaaac ctcccggatg cgttcagccg
    13381 atcacgtgtt ccgtttcggc gtcgaaaaac agcgcgtgcc gcatgtcgag cggaaaatcc
    13441 gccaccgtgc ccggtgtcag gacacggtcc ggatggacac gcgcgatcag gcgcgtcccg
    13501 ccttcggcca caagctggac aaatgtttcg gcccccatgg gctccacacc gtccaccgtg
    13561 ccgcgcagcg atacgccccc ggccgcgtca tcggccccca gggcttccgg ccggaaaccc
    13621 agcagcacct cgcggtctgc ggcctgcgcc agcgacgaca tggcgacagg cggcaacggc
    13681 aaccgggcat ccggcgcata ctgaaacagg agcgtgtcgt ctggttgtcg gaccagtctt
    13741 ccgcgcaaca cattcatggg gggcgtgccg atgaatcccg ccacgaaccg gttggccggg
    13801 cggcggtaga cctccatcgg gctggcggcc tgctggatgg tgccgcggcg catcaccacg
    13861 atccggtctc ccatggtcat ggcttccgtc tgatcatgcg tcacatacac catggtcgcc
    13921 gcgagacggc gatgcaggcg ccgtaactcg ccgcgcatct ccacacgcat tttggcatcc
    13981 agattcgaga gcggctcatc gaacaggaag accttgggtt ggcgcacgat ggcgcggccc
    14041 accgccaccc gctggcgctg tccgccggag agttgtttgg gcagccgccg caacaggccg
    14101 tccagtccca gaatgccggc tgcctcctgt acgcgcgccg tgatctcggc acgcgggaac
    14161 ttgcgcaacc gcaggccgaa tgccatgttt tcgaaaacgg tcatgtgcgg atagagcgcg
    14221 tagttctgaa acaccatggc cacgtcacgg tccttgggcg gcacggcgtt cacgcagcgg
    14281 tcaccgatgt ggatcgttcc ggacgtgatc tcctccagac ccgcaatcat ccgcagggtg
    14341 gtggacttgc cgcatcccga cggccccacg agcaccagaa actcaccgtc gcggatggtg
    14401 agcgtgaacc ggtctacggc caatccgccg tcatcgtatc gcttgcagac cttgttcagt
    14461 gtgacttcag ccatgtctct tccgtggggc ctccctacag caccaggcgc acgccgggca
    14521 cctgtttgat ctccgcgtcc agacgcaagt gttcttcccg gtcttcgagc ctcaccgaaa
    14581 cgcagaatgc gcggtaacgt cccgttgacg agtcccgcga gacagcgagc ggcgaggtga
    14641 cccggtaggc ggcgatccgc gcggccagcg cggtttcagc atgcgtcgcg gtttccgtga
    14701 gaatccggaa gtggaactcc ccggggtagg caggctcttg cgccgcgggt gcggcgtcgc
    14761 ggttttccat acggggggcg gggcgacacg aattcatgag actccttttg ccggggttgt
    14821 ggtctggata ctcgccgacg cgagcaactc gcgcgcatgg ctctcgatca cgctggtcaa
    14881 tcccttgccg cccagcatgc gcgcgatctc atccacgcgg ccggcatcgt ccagagtcgt
    14941 gatgcgcatc cgggttcggc ctcggaccac ctcttttgtc accaccacgt gacgatgccc
    15001 ataaacggca gattgcggca gatgggtgat ggagatcacc tgatgggttt ctgccacgcg
    15061 gcgcattttt tgccccacca ctctgcctat ctcgccaccg atgttggcat caatctcgtc
    15121 aaacaccagc acggggatcc ggtcgtgcaa cgcgagcacc gctttcaccg cgagcatgac
    15181 ccgcgcgatc tcgccgctgg aggcgatggc tttgaggggc cgcatcggct cgcccgggtt
    15241 cggcgcgaac ccgaaggaaa ccgtgtccgc gccgttcggt cccggcgggc agggactcag
    15301 ttctacgtcg aaccctgatt tcaggaaacc caggtcgtgc agttcttgcg tgatcgagcg
    15361 ggcgagtgcg ccggccgcgc gcgcgcggac ggtcgaaagt gcggcggccc gcctcgtcag
    15421 ggtttgggtt gcgtccgcga tctccttgcg gagcgcatcg gcctgttcgg tgcgggtggc
    15481 gagatcctgc aaccgcgctt cgctcttttc cagggtggcc aagacctcgc tcactgaaga
    15541 accgtacttg cgtttcagcc gctgcaccag tgccatacgc tcttcaagat ggctcaacct
    15601 ttccggatca gcctcgatcc ggctcaaaca atccgtcacg gcccggccga gttcctgaat
    15661 ctgcacggcc gcattccgcg cttcggcgag ccagtcggcc gcttccggca gaagccgcgc
    15721 cagttccgtc agctgcgtct gaaccgcgac cagtccgctg aacacggctg gttcgccatc
    15781 ggtgaggcct tccaccacgg cgtttccgag tgcgagaatc tgttcggcat gcgcggcttg
    15841 ggcgtgtcgc gaaaccaggt cctcttcgtc cgcctccgtg agctgggcgt gggtgatctc
    15901 ttccgcgatg aatcgcagtc tttcggtttc cgcctcgatg ttcggagcgt cgcgttgcag
    15961 ttcgtcgaga cgcgcctcca acgtctgaag ggtttgccag acctcggcac aggcttcgcg
    16021 ttgcggcaca cagtgcccgt acgcatccaa cacatcccgt tgaaaggcgg agtccaacag
    16081 cgactggtgg tcatgagggc cgtggatgtc caccagcaga ttccccagcc tgcggagcgt
    16141 ctgggcggtg gcgggcgaat cgttcacaag acagcgtccg gcacctgaag cagaaaccga
    16201 acggcgcacg agcagcgcgc cctcttcaca gggcggcagc cccgccgact ccaaaagcgc
    16261 atccacctca cgcgtatcgg caagcgcgaa ccccgcctct acccaggctt ccgccgcgcc
    16321 ttcgcggatg gccgacttgt cggcgcgtcc ccccaagacc agatcgagcg cacccatgag
    16381 cacggatttg cccgcgccgg tttcgccggt gatgatgttg aggccggggc cgaaggtgac
    16441 ggcagcctct tcaacgatcg cgagattttt gactgacagg tggttgagca cgacttttta
    16501 tcacattcaa cggattcagg atcagacaag ggaagtgtaa cacatccgcc gctacgccgt
    16561 aaacggtatg aaaaagccgg gcaaaaatac gaagggacag ctggagcgct gaatgtcgaa
    16621 ttgcgacgct ttatcaggtc gaggatgcgc cccggttacc ctgtccagcg taagcgggct
    16681 tgcatccgga gtgttcaaac gccataatct gttctcacgt tttgatgtaa aaggagagtg
    16741 aagcatgcgg aagaagattg ttgcaggaaa ctggaagatg aacaagacgg cttcggaggc
    16801 cgtggccctg atcgaggaga tccgaaaaga aaccgaaggc gtttctgcgg tggagatcgt
    16861 ggtgtgcccg ccgttcacga atttgaagga tgcggcggca gcctgcgccg gaacccatgt
    16921 gatgctgggt gcgcagaatg tgcattggga ggcttccggt gccttcacag gcgaaatttc
    16981 aggggcgatg ctcaaggacc tgggcgtgca gtatgtgatc attggccaca gcgagcgacg
    17041 ccagtatttc ggcgagaccg acgagacggt caaccgccgt gtccgggcgg cattggctgt
    17101 ggggctgatc ccgatcgtct gcgtgggcga gactctggcc gagcgcgatg ccggacagat
    17161 ggagtccgtg gtggtgcgcc agaccaagga ggggttggcg gacctcggtg aggatctggg
    17221 acgcgtggtg atcgcctatg aacccgtttg ggcgatcggt accgggcgga cagcctcgcc
    17281 ggcccaagcc caggaggttc acgcgctgat ccgccgcaca ctggccgaga tcgccggagg
    17341 cgatgtggca aacacgatcc ggatccagta tggcggcagc atgaagcccg agaacgccag
    17401 agaactgatg agccagcccg atatcgacgg cgggctgatc ggcggcgcgg cgcttaaggc
    17461 cgactccttc gcggcaatca tccgggccgg catgtaaggg gtttgcctta gcggatctgg
    17521 gcgacccaaa gagacggggt gctttggcga ggcgcccctc ggttccggtt tatcgaaatc
    17581 gttgtcgggg tcggcgtctc gtcctcgttc atgtgaacgt cggcttatga cctgtgtgtg
    17641 accttcgccc cgcgacgttt cagattttgg aatccgttta ggacatgatt tccgacagct
    17701 tcgcttcggc ggggagaaat ccatgattac catgatgttg ttcaatcctg aaaatcctgt
    17761 catcctgtca atttataggc aagagttgac cgttccggac cgtccgcagc gtgaaacccg
    17821 agccccaaaa ccaaagagcg ctttgaaggt tcagctattc cagggttcca atcgggttgc
    17881 cggattccta taatgggcgc tcatggagag ggaaacccat cttgttgagg ggagtgccgc
    17941 gcagatgcgc gtgccgcagg aggctttgcg gcggcgtgcg ctggaagagg gcgttgcggc
    18001 gtatgtggcg cgcgagcgtc tcgttccgcc tctgactgcc gccgaaatgc tggcgcatgc
    18061 ccgtgcggcg accgcttcgg aaaatctgga tgatgcggag gcggtgttcg cggcggttct
    18121 gctcaacaat gcggtttggg cggatgtggt cgccggtatt ccctatgagc ggcggttgtt
    18181 gctccttccc ctttgtttgg cgcggaacga ggtgtgccgg gcggagcgcg acggtttggg
    18241 tctcttgtgc gcgcaatgcg gcaactgtcc gatcggcctg atccaggcgg aggcggaccg
    18301 gctcggatat gtctcgttgg ttgcggaggg caccacggtg gtgacccagt tgctcgccgg
    18361 cggcaaggtg gacgcggtga tcggtgtggg ctgcatggat tcgctgcgcc gtatgttccc
    18421 cattatgaac gcgcacgcga ttccgggtca ggggatcccg ctgctgacgg atggctgtca
    18481 ggccacggag gcggatgtcg catggatcct gcgtgttttg cgcgcgagtc gtcagggggg
    18541 cgtgtcgggg gtcacggaga tcgaggcggt ggccgatgcg gttcgcaact ggttcacgtg
    18601 cgaggagttg gccgccgtgc tgggctcggc ggagacggaa gcggagcgga tcggccggga
    18661 gtggctgctg atcggaggca agcgttggcg gccgctgttg accgccgcgg tttcggaggc
    18721 gctggggggg gcgcgtgacg ccatccgtcc tgcggcgctg gcggtcgagt gtttccacaa
    18781 ggcgtccctc attcatgacg atatcgagga tcaggacacg gagcggtatg gggtgccgac
    18841 cgtgcattgc cggtacgggg ttccggccgc gatcaatgcc ggagattatc tgatcggtga
    18901 aggctaccgc ctgctggccg aggccgcgtt cccggatgac ctgcgcgcgc gtatggtgac
    18961 ggcagcggcc cgcggacacc gcgaactttg tctcggccaa ggtgaggagc tggcgttcag
    19021 ccgccagccc gcgccggtga gcgagtccgc tgtgctgcgt atctatgagc gcaaaaccgc
    19081 tgcggcgttc gaggtggcgg ttttgctggg cgcgatcgca gccggagccg atgcggacac
    19141 gtgcgcgcgg ctttccgaat tcagccgtgc ggtgggtacg gcgtatcaga tcggcgatga
    19201 catggaggat ttcttctcgg cgcgcggtcg ggccgcggac ctgctcgcca tgcgtcccac
    19261 ggtgtttctg gccacagcct gcacatgtga caacgcggaa gtcgccgcag cgctggattc
    19321 ggtctggaat gcggaccgcg ccggacggca gcggctgatc gacgcgatca cgcaggccgg
    19381 cctgcatacg cgggtccgcc tgttgtatga gcattatcgg catgagacgg aacggaccat
    19441 cgccggaatt cagcataccg gcctgcaacg cctgttgcgg catgtcatgg ggcggatgct
    19501 gcgataggcg ccaggcggcg cggaccggac ggactgatga ggatacggca agccatacct
    19561 ttttgcgtga cctgcttggc ggcggtgtgc gcggtcggga cccttgcggc atggctcgcg
    19621 cgcgaatccg ccgtggacgc gtccttacgt ctggcgaccg ccgacggctc gggtgccgag
    19681 gcggcggcaa tcacggaatc agacgcgcgc ggaacggatc tgaccggttg tttccgcgcc
    19741 aaccgggaga tcccccgggg agccacgacc ggaaactggc cggggttccg cggggcgggc
    19801 cgcgacaatg tcgcgcgccg ggcgggaaag ttgctgaccg catggccgga aaccggtccc
    19861 gccgtactct ggcagattcc ggtgggggac ggacatgcca tgcctgcttt ggccaacggg
    19921 tgcctctatc tgctggacta tgacgaggcg cggggcggcg actgcctgcg ttgtctgaac
    19981 gcggacacgg ggcaggaacg ttgggagcac ctctatgcgg tcaaaaccaa acgcaatcac
    20041 ggcatctcgc gtaccgtggc cgcgacggac ggtcgctccg tcgtcaccat cgggccgcag
    20101 gggcatgtcg tgtgtctgga cgcggatgat ggcacatacc gctggggatt cagtctgtct
    20161 gaacggtacg gttcgcgcgt gccgctctgg tatgtggggc agtgccccct gatcgatggg
    20221 gatcgggtga ttctggcgcc tgccgcatcc ggggttctgc tgtgtgccct cgcgctttcg
    20281 gacgggaaaa ccgtttggga gacaccgaat ccgcacgggt acgcgatgtc gcattcgtca
    20341 gtgatgattc ttgaaacgga agggcgccgc cactatgtgt acgcggggat cggcgggatc
    20401 gcgggcgtgg gcgcggaggg cgcggaacgg ggaaccttgc tgtggcacac ggaggccttt
    20461 cgtcccagtg tggtcgtgcc gtcacccgtg ccgctgccgg acggccggtt cttcatgaca
    20521 gcggggtacg gctcgggtgg cgccctcttc cgggtgtcgc gcgtttcaga cggttggcgg
    20581 gcggaaacgc ttttcaaaac cgaccgcaag acgttcgcga gcgaacagca gactccggtt
    20641 ttccttaacg ggctactcta tacggttctg cccagcgatg ggggcggcga gcggcagcaa
    20701 ttggtttgca tgaccttgga gggtcagcgg atgtgggcga gcggacgcga ccatacattc
    20761 ggactcgggc cttacgtcgc tacgccggaa gggatgttgc tgctgttgaa cgatgccggt
    20821 acgctgacac tggcgcgggt ggggcgcgac gggtttgtcc ccttggcgcg gcatgctctc
    20881 atggagggca ggggacgcga tgcctggggt cccatggtgt tggcggacaa ccggctctac
    20941 ctgcgcgaca gcaagcggct cttctgtgtg caagtcggcg caccatgagg cggccggtgt
    21001 tcagacgaca cgctcgaccg tgatttcggg gtcgatctgc tcgcgcaaaa cccgttcgat
    21061 accctgtttc agcgtcatgg tcgcgtgcgg gcaactgccg catgcgccgc gcagacgcag
    21121 acgaacggtc ttgtccgtga ttgaaaccag ttccatatcc ccgccgtcag cctgcagcat
    21181 gccgcgaagt tcttccagtt ttgccttgat ttgctcgtcc atcattgttc cttccgcttg
    21241 aaatggtggc gtatcatgcc cgaaaacaca gggtgcgtca agttgcggtt gacgggttcc
    21301 ggtggccgcg ctaaggttgg gttcgtgaat cacaaagccg aaactaccgt gttgctcgcg
    21361 aacggcgcgt atccggtcca tccctttccg gtagacgcgc tgcgatcggc ccgccgtatc
    21421 gtttgttgcg acggcgcggc ggaccggctt tcgcaagccg ggctggagcc tgcgtgggtc
    21481 attggcgatc tggacagcct ctcgcctgcg gcgcgcgtcc ggtggcccgg ccgcgtggtc
    21541 gcggtttctg atccggagag caacgacttg accaaggctt tccgcttttg cgtatcccgc
    21601 ggctggcgcg atctggtgat cgtcggcgcg acaggccttc gcgaggatca taccttgggt
    21661 aatctctcgc tcctcccgga tttcgcgcaa gaggcggagg tgctcttgtt gaccgatacg
    21721 gggacgttcc ggccgatgtt gcggtcagcg cgcctgcgcg cccatcctgg acaacaggtt
    21781 tcgattttct cctttgatcc ccgtaccgtc atcacgtcgg aagggttgcg ttatccgttg
    21841 cggcggttac gactctcccg ctggtggcag gccacgctga atgaggcctg cggcgacgag
    21901 tttgatctga tattcgaggg aggccccctg ctggtgtttc agacgtatga gatacggccg
    21961 aaccgtgcgc cggggggagg cgatggggaa atggcggggc aacatgaatc gaattctgtt
    22021 tgagcccggg gaattgtgcg cggatgggac cgtgacgctt gcggacgcga gggccgagca
    22081 catactgaat gtgctgggac tcggcgtggg gggcgtggtg cggagcgggg ttgtgaacgg
    22141 gagtgccgga acgtcgcgga tcatcgggac ggagggaaag cgaatccggt tgcagaccga
    22201 tcatacgcag ccggcggagc cttctgggtt cgatttgttg ttggccgcgc cgagacccaa
    22261 agtgatgaaa cggctttggc cgcaattggc ggcactcggc gcaggccggg tggtggtgct
    22321 caacgctgcc aaagtcgaaa agtgctattt cagcagtcaa tggcttgatc ctgcgcggat
    22381 ccgtccgctt ctgattgaag ggttggccca ggccggcacg accatgctgc ccgaagtgcg
    22441 ggtgcgtcag cggttcaagc cgtttgttga ggatgagttg ggcacccttt tccccacgcc
    22501 gttgcgtctg ctcgcccatc ccggaccggt tgtcggtctt gccgcttccg aatggccgtc
    22561 cgggacgcgt ccgctgattg cggtcggccc cgaaggggga tggaccgatt acgagcgcgc
    22621 gatgctgacg gagcgcggtt tccgtccctt ttcattgggc ccccggactc tgcggaccga
    22681 tacggcgtgc gtcgcgctga tcgcggtgct ggcttggctt cgcgactcct cccgctggag
    22741 cggcggagcg gatgaatcgc gtgttttgtc cgggcggttg gggattggac aggttgccgg
    22801 tccttcatgt actataaacg ccgaacgcag aacggaaaac catgaaacga gaacgctggg
    22861 ttcgttatcc tcagaagcgc gatacgccga ctgagtgccg cgactggatt tacggtcgtc
    22921 ggccggtatt ggaggtgctg aaagccggta gacgccatct gtatgaggcg gttttgcctc
    22981 ccgccggacg cgacgcgccg gaaatcgccg aaatgcgtga cctgctgctg gcccggggtg
    23041 tgccgttccg cacgttggag cggagtgagc tggacgaatc ctgcgagggc ggcaaccacc
    23101 aaggtgtggc cgtgcgcacc ggagggtatc cgtacatctc gttcgatcag gttctcgacg
    23161 atgtgaaaac ggatacgaac gccgtggtgc tgattctcga ccacatcgag gatccgcaga
    23221 atgtcggttc gctgttgcgc acggcggatg cggcgggcgt cactgcggtg ctgattccga
    23281 aagaccgcgc ggccgacgtc acacccgctg ctgtgcgtgc ctctgccggg gcctccgagc
    23341 acctgcgtgt ggtcaaggtc gtgaatctgg tgcgtgccat gaaagaactg caggcgtgtg
    23401 aagtctggat cacgggattg gacttcggac cggacgccaa accatatacg tccatcgatt
    23461 tcaaaggtcg cgcggggttg gtggtgggca gcgaggggcg tggcctctcg cggctggtgc
    23521 gcgaaacctg cgacttcatc gccagcctgc cgatgaaagg ccgcgtcgaa tcgctcaacg
    23581 ccggcgtagc cggcgcgatc gcactctacg aggtggt
//
LOCUS       DAOSRB010000017        23368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2362_length_23368_cov_8.151418, whole genome shotgun sequence.
ACCESSION   DAOSRB010000017 DAOSRB010000000
VERSION     DAOSRB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23368
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2362_length_23368_cov_8.151418"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2371..4257)
                     /locus_tag="PLZ74_01980"
     CDS             complement(2371..4257)
                     /locus_tag="PLZ74_01980"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97121.1"
                     /translation="MGRLTVAAVSVWVAGVCISAQCSSALYGADAGSSPRGGGGCDAW
                     YYPQMSMELYRLEELKRNAQAAGMYVGYPGSVPPYAAAVEFRRRFKPGETREYFTHGR
                     AFSENFDEKTGMLRVSVSARYLDTPALRSPLKGWEARVSGPQRDDTWRAAARYSGGEV
                     PPHLRWLEEDHDYRDLKKIGDYYDTQRELLAYVQTSGEKPPKLFVGESVPEMRNTVPV
                     DSEQATDMVLVANDTWRSRSALALRYFRFEGKAEKVKVIPLWWPGMEEKGTFSAKDKR
                     HEKMREVGVHTLKLCCHDFLIDGIKRDRLPWGGDLTVSLMANAYVLGDPEISRRSLSV
                     LDAYTTDVNGIVTYSMWLIIAHDLHQLYFADREFLEARWWRIKQRIEDLISRTDENGF
                     VARGLDWVFIDWAGPRSATALGMIWTGALDAAAKLAGRINDPRAAEYRALCDKVKANL
                     NRLAWDEGRKLYRVDPFEKNSGFARQANIYAVIFGVAQNERAQAIGDELAKDDLPAVG
                     TPYVFGWELVALARTGHHKEFFTGMETVFGAMLDAGATSFWEGFNAKQKGDERYRFYG
                     RPWAKSLCHAWSAWPAFIFVSEAMGVKPTSDGWKTHEVKPIPGAEGMKADIPTPSGRT
                     KIGL"
     gene            4562..6463
                     /locus_tag="PLZ74_01985"
     CDS             4562..6463
                     /locus_tag="PLZ74_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOR97122.1"
                     /translation="MRLDAIVRLLLTVSFVAHVRCFAGPVVTPFMERTRPGAVRPEGW
                     LLDMARKARDGYTGHMDAVDVQFSRSWRNETRPRGKDLLWVSDPGAWSCEGGAYWFDG
                     LVKLAYQLDDPSLKALASNRLDTVLSEMNPHAIGFCWWLDRNDPQQVLEMKISDAWLI
                     WVTGMFARPVCAWHEVSGDARALKALDFALDEHAFSYGKFPTLPSGAYAGYQVTRSER
                     IARALDEFWKKALHQPGQLPAVFSQYLTPPPAHLEETLNIRRLHERLLKIPTRHGVIA
                     SEALLSIFDGYLWTGNTNLLNAVRGWYAFFDRHMRQPYGVTTMDEEWGHPGPERGTET
                     CVVAAEGWTRINLLAALGEGCWGDDVERNFFNAGRNCMTEDYRRHVYLQQPNRLTASD
                     VTRCSFTGARGDRLARYDTKHWPLCCTAALNRILPNYVASMWMLPADGGVAATLYGPC
                     RFAAELSSGSFEVEEHTDYPYSDTITFKIVKSPNAAFPFHVRFPGWCANPELQVNGRQ
                     TVIAAQNGFAAITRIWRAGDVVTLRLPMKPTVEKMFNYNEPRGREYASVSVGPILYAL
                     PISSKDDNNPEGDVHVPLVSAGFDAASVRVGDAMRMLTIPDSKGNSLKLVPYGDAKLR
                     ISMFPVDGD"
     gene            6692..8680
                     /locus_tag="PLZ74_01990"
     CDS             6692..8680
                     /locus_tag="PLZ74_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97123.1"
                     /translation="MKETNVIRVLPALGCGLLAFAASAVEINVASVTDLRAAIADTVN
                     VPDGSTIVLQASPTHYDVSADGLGPIRLTRSLTLRGSTENPRDTVLTGNGMTNILYVG
                     GTDTLACNLTVTNGLSYLVDSRKWYRGGGIHVNGSSAVVSNCVVTGCHFDTRTDYLTG
                     TAVAEMTAFGGAGVYANGSSLVLDTLIENCWMFNDRKQKVTGAGAGLFIGGKAWGTDT
                     PSRAYRCEVRNCSTETMSVGGGTGALVGGGLYVSDAGIVDNCRVHHNWVRPTDDSSRC
                     YGGGLYVGTGGIVSNSLVYLNTCQKCVTNTSGNASGGGVAIEGAGLFCTSTVSNNYSY
                     GDAGGVFHINYPSGGLRDSLIIDNEAVGSGGAGTGGPAATGCVFHRNTSGGGGGGWLT
                     GGSAGWFSNCVFEANATGKWGEGKAARVQRGGSTPFVFESCWFVNHTVTDGKAVLQFD
                     GSPLPTYRAHIRNCVFVGNTGSCPFYSSYTATGPEIDNCTFVASDTEYYAIGAGSKRE
                     TNVVVRNTLFHGYKNGGLPPAFATDKPEHVSNCYDQVATTLPKDGDGNILNGNLAGTI
                     VGEPGFRNLAETNLTLRGASPLRGKGLVLDWMKTQDGSADWTACDAGAGTYMIEKVAD
                     YGVAVRFGRRYPRYTGGADAPDIGAFEHYGTADVMYIK"
     gene            8709..12539
                     /locus_tag="PLZ74_01995"
     CDS             8709..12539
                     /locus_tag="PLZ74_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97124.1"
                     /translation="MKRSLAMGLALAAVLPCVEAGMKTILDVPVAGVTNTAYLTGMAS
                     RPWWNAAWKRRAPILVSSHADVPEPKAVVDCVVDFGEKVNPDEVRVVTPWETVVPCVC
                     EKGKAEGQGQEPAAVRLLFQTPLRIHENKPFLVYWGNPAAKRETPVTALMMSETANEV
                     RILNGVLDVTIDKKHVTGGFLRKLRIMGTDASNELLERATQWAWNGLEMTLGGATDWS
                     DGVVTEDNAFVKRVRFEGTNGAVTLSVYADQPRVDWAYALKKGDRCELNVAWAVGGGV
                     AYDKFVYPGATGRLLTFDAALDSPTDSIPQPRFEMAPWFGEGWYAIASKRSPSVCGLL
                     FDRPALQGLWYNGSGQAFGEWTRLTFAHKARKDAPVTGSGSLVALPGQALDVRREYLR
                     RKTPLTVSVGAAQPYAEYPYVRPRLDRDFIADINCGKDSGHGWRSGKPLPDPAWASNI
                     VDHLRSYGVTGIRVGGYFWWDMPLTEKLYRDLERQANRPLVAPDCRKRFTGWDPDQYR
                     GTDFLEMANAAHAKGLVVKQCGDFIPGMYGREFDTKFDADIQRTALDLHLRMAEVGGV
                     DVIYNNFEGQEQVILPQDVKREKHNDYWNWKDPDEFFAWQDKARVLVKAFYDAAKKAR
                     PDLPVLVYNSENGELSREIFMSEQIGSFDTVVVEMLSGLDFSRCKHVAKRMRALFDNE
                     AGRTVHHHYYFMKPDAMHRIKEIELPFICGVNGFSHENQSYENFSREQSEIAADFYRF
                     AEYTRLGEKAARMAPVRYLGVFRDSVCYREDIRKGRHGQPKDRPYFNRAEQDGRCRAL
                     GEIRNLDFDFVMNPFFTAKALAKYRIVYVPEDDVFSDALAKELVAYVKAGGTAILEAK
                     TAGNTVIKSLGLKDGVITPLGKGKILWYEKVRTDGLAKRDEKTIREVAAAIEALAGPM
                     PHRIDGASSLDGVLQSGSDGMLLGVHNAGDKADAGRVTIAKALRSGPSLYVLDVKTGV
                     RFPYTNGFDIAIGPRQCGFYLIGDETFTAVPETKTAVWNGPAVAAARPNGIPAQVNDD
                     PSFVPAKAVEFTRGDGKGNPTAIRRSREAMIDVTPVTPADCYPPVLARALSTATYIHF
                     SSLGTLSADEKKTVDDVFVSCADELKALLKRGGTILFDRSPAGPQTRAFLKEIGVFDP
                     YAHPAPKLGDENTVWTSETNHPFAGNLAVKKGGFGRFQATERSFAKWDAARQVAPVRM
                     VRNHDVAVFVLQENVLGTGRVVFLENRFAFSDWYENRAFGDALLGWIVGRPIEEHAKK
                     SLQRAGGIGVALSE"
     gene            12554..15259
                     /locus_tag="PLZ74_02000"
     CDS             12554..15259
                     /locus_tag="PLZ74_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97125.1"
                     /translation="MMKHMLLLTACALTGARLLPAFGDVTPAALPGGYTELPYVESTY
                     NGGQWVYTDYVPEWNHRIVCKFRVNQVGTWNSPGVFCARGTATNDKGYCLSFGQGGNN
                     RVNLPGALQCWTGGWAAPSSAPFFYPDLLTDYEVDVNVELKKWCVDDGERWNTREHTS
                     FTAGSPLAIFCRHELGNSLSPSSTFGANNCMAGRIYYLRAYDANGTLIHEFVPARDDN
                     AMVPTKYFGFYDTVDAKFWPNCGATAFATDPSVPSNVRVVDRADYDTDEEAGTALVAT
                     VAAAFPTDRIKVTGGTKAAPHVYRITSPLLMEMTNTRLFGDSDDAEATVIDAGGTSAG
                     VVVKGSGISVSGLTVRNATNYVNGTTYCGGGLALIAEGSVASNMIVCGCVNGGVEKAL
                     RGGGVYLYKSTLVDALVKDCVISNDMDTTGKTAFYGGGVYAERSTVSRLEVSGCLAVN
                     NQMCESMWWATGAFRGGGLMLNESSATDCYIHNNMITNGPKQTSSCNVSHGAGVALMG
                     ASTLSRSLVTGNSAPCSAAGVNLESASAVIDCTISSNTVFFAWSGAYPGGGLYLRNAS
                     QRAVNCLLEGNRNLGTYWDAGGGAGVIEAGVFLGGVVRGNGSYNTHGGGFFLNGACLV
                     SNVVFDANWSTDSSDRCGSGAIEFRGGGAVVRDCWFVRNIGGDNANNSYGTVFYGSYD
                     TGSVLQNCFFANNTGACVLGGSIETNKADCVAEACTFVKNHVSRGAVWCTALDYSVPG
                     TVQKMAFTNCVFWKNGENEDKPGIPDKFKNVSTKVSYCYDEIGTGLTPGDYHNLTAAN
                     GEPFYADAENGEWRIGRKASVLVDNGLKLDWMGDGAKDGPLDMGDGTWSEAPAATISV
                     RGKTMDVGVIVTRNNAQPRISPQNGLPDIGCAEYQAVNGFILRVH"
     gene            15328..19230
                     /locus_tag="PLZ74_02005"
     CDS             15328..19230
                     /locus_tag="PLZ74_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97126.1"
                     /translation="MRSFNFSHAVLALSTILIGASASALDILDVPVAAVSNRVYLADM
                     PERPWWNRAWKRRAAILVSNMSDVREERAMIDFIFDVSEAVEAKSVRVVTPWGTAVPC
                     VAEPVRQGEGALITARSRDVPALVAPTALRLLFETSLRERENRPFFIYWGNPDAPTQG
                     IDSPMTLSCREDEVRVNNGIIEVVFDNLRRTDGLMKKLKVNASQAPSELLWRTTGFAW
                     RGFEFSAGRAPKPWSKAVITADNAFRKTVTFTNEDAVLDFTFYAGQPRVDYVYRLKDG
                     LHNARIDVSWACGGDCGHDDFTYRGLSGQLLTFRAALDHVTDCMPQPEYYRFPWLSEG
                     WYAITDRRNRDVVGMVFDRRALGGVSYIGQGQSSGEYVSLNFNHAPKGKTPVTGSGAL
                     VATVGEARTVATLYAWLTHPPKVFVAKAESYREIPVKVPVLDRDFCVNVNVGGWRSST
                     PLPGDEWAPNIIAHIRALGANTILLGQLTDYSWTSLPISKDLFDRLCAHKKARNPSWK
                     APEWKGGYDGRLLKRICAEAHSNGLAVCTWHDALPGFSYGGKFDAEEQALCREMQALY
                     PMCGVDSTFNALGGGEGVEFPDDDLKKFGRAYWKWPDPEPFFASRRLSGEYMKAFYEY
                     SHKVNPGTKVMVFNSENSELERDMCMGYYPGVMDTLFCEFVAGMNLSKIKHTAKRLRG
                     YFDNRDGHTIHAHYYNMTKDYAHRIGQLEMPFICGINGFSQESMTYENFDRDLFEITA
                     DFNRFALYTKLGPKVAKMAPVKNLAVLRDMACFEEDCRKYRTMPRDWWHASRHDARVN
                     AFAEILSYNYDVVINPHFKAGSLARYKAVYLTDDEVLPEALAAELVAYVKAGGGAVIE
                     GSTGERLNVEEVKGWEDGKVIELGKGRIVWTKEVLTDPMMKGDAKAIARVKALVASVG
                     GVAPFEIASKSLDGILQAGEEGMFLGVYNKGKTEDRGKVTLYVGSPSYVLDVKKGVRF
                     PFTNGFEIAVGPRQCGFYLIGGDAFTAIPAAQEAAWMGAALASVRPAGQKPPENPDPA
                     FRPYVAVEFTSVRGEGAKAKAAGITRSRLAGIERRRFVTDEATLCRTLKVDDLDVWRS
                     NNTDVKTYSAQAFAAALKTAHYVHFSANPKDCDAAFADCAEELKALLRRGGGLLFDRN
                     GTGPAARRFLAEIGVFDPYPTAKRGIGDGAPAWDETFPTNHPYYATNETKPRYGVQRL
                     EADGGQYSLAFTKWDPQTQLAPFRTARDPSAAELVIQDKVLGAGKVVFNQNSRSFNDW
                     YETKTYGNVILSWFIGMPVDEHAKKARFFVGGIGESVK"
     gene            19245..23123
                     /locus_tag="PLZ74_02010"
     CDS             19245..23123
                     /locus_tag="PLZ74_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97127.1"
                     /translation="MMKGRVKQLVIAAVLAAGSVEAKTALDVPVSGVTNKMYLSSAAT
                     RPWWNTDWARRAPLLVSSMADIQSGAGWVDCVVDFGETVNPDEIRLVTMWETEIPCVA
                     EKVDGGRVRLIFQLPLRIRENKPLLVYWGNPKAEKPTFIETMNVTMDGATARLANGRV
                     ELTIDRRQEAGNTIKGFRLVGSQTGDLVTLRATGAPWKGFTFATGRSNVVWKTCRVTA
                     DNPFKKQVTLDAPEATLVWTMYPGQPRADYAYTLKRDPSARIVVAWCPGGGAAWDDFY
                     YTGLTGARLTFRGSLDEVTDSGGFPHNDHLEKWMKEGWYAICDRKNGDTAGMVYDRAS
                     LARVSYGAAHYNGGERVSLTFEHASKKGESLRGAGSGALVGTFGGADQVAAEYARLVA
                     PPQVFLGKAESCRTIPIRLPDHTKDFMGEFVTGMNPGNWEHLRPGTNPDWADNAANYL
                     RRFGATAIRLTGYGWQELCLTEDLYNRCKTIIREDPKIADHVKNRPIADWSVERYAGV
                     NLRKVSEAAHRKGLGVSTWTGRGAEIFGGLEADVTETPLYRQLDFDLQNRYPKSGAGV
                     VYNSQCSLEGPWMPKALRQERGNEYWRWEKPEDFFNAQDDFIRYMKAFYAQSKRENPK
                     TPVFVFSAENGGLNRELPLADLIGAFDAEYCELVPGYLASAQGIKHSVRRLRSHFDNN
                     PHTAFHHFYYYTSDHGWRISQLEVPFAFGVNGFSHEVMMHDTIDVELNEITGDFYRFA
                     EYTRLGGKVSKMAPVKHLAVLRDARLFRENVLNKRPNEQESRLRGFAALRNYPCDIVA
                     NPFFTCDSLKRYTIVYVPDNRVFTDEQAAELLAYVEAGGNAIVEGVTGEKVKGLVDGE
                     ITVLGKGKIVWSKEVLSEQLAKDDASKVLPLLRKLGGEEPYSVKAGGSCDGLLQASGE
                     GFFYAAYNVAKHPSKGRFVMKEEVRKSLFVLNVRTGGRKPFDGSFEFEVGAQQCGFWL
                     IGDEAFTAVPEAKACVYDGASAVSAKPAPVKLPQHDVEKAKAFKELEIVEVLSLNPGF
                     LGYQTGGEIGRIDRHERLKLKLTRFYPDEKAVAEVVKPEELDTWKQINKGLYNPFDET
                     AFAKALKTAKVLYLRGSAAQDLPFVRCPQAVKDFLAGGGGIIFDCLDANTNVTAFLKS
                     VGVEDPMAAGRFVKSCGQRAVKAPGLEHPIVRDTNERINDFNREGFCRYHLTGGGWKG
                     WSKDQFAPVRAFEDPEQIATVIVQENVCGKGRVVFSRNWNAWWSYRSSMCDGLLSTNI
                     LSWLFDYDVNAYESDLQVKNGGPGEVVGEQ"
     gene            23185..>23368
                     /locus_tag="PLZ74_02015"
     CDS             23185..>23368
                     /locus_tag="PLZ74_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97128.1"
                     /translation="MAAPPKAAAASATVAKPPASPSRVECRGSRGDDPDPDPDLDETL
                     KHMKLHENQEFPLAFQK"
BASE COUNT         4437 a         7004 c         7590 g         4337 t
ORIGIN      
        1 gacgatcgag gtgccggtcg atacggcgct gcgtcccggc agttatacac tacgactgcg
       61 cgtcgagacc cctttgaagg aggcgagcga tccttatgtg agcgaagaga gcgtgacgtt
      121 cacgctgatg ccgcgtcgtc caccgcgcat gccggtagtg atgtggggcc tttacggagc
      181 gggcaacgtc atgcgcgagc taccgcgcct gacggaaatc gggttcacgc actgtctggg
      241 gttcggcgcg gatttcaagc gcatctttga cgcggggcag ccggtcgagc ccgagacgcc
      301 tgagcgcgtc gcggcggtca agcagatgct ggacaccgcg ctggcgcatg atgtcggcat
      361 catcgcgacg ctttctcctg gccactgggc ggccaacgcc aagccggagt tccggcgcgt
      421 ggatcgcaag ggcgtcagca acgacaacag accggcgctc tgtgcggcgt tcccagaaat
      481 gcagacgttc gccttcaatg taggcgcatc ggtggcgcag gcctacagcg actttccggc
      541 ttggcaggca gctctgctgc ataccgaggt gcgcgacggc gcaaacgtct gctggcacgc
      601 gcatgaccgc gaggcgtgcc agcgcgcgac cggttctgac tatccaacga atgcaccgaa
      661 caagaacggt gtggagtaca tcaagctgag cgattttccg tctgacaggg tgattcctga
      721 tgaccatccg cttttgcgct tcctctcttg gtattggaaa gagggcgacg gctggaaccg
      781 catgaactcc gagttgcacc gggggctccg gtcgaccggg cgggaaggac tttggacatt
      841 ccacgatccg gcggtgcgtg tggcgagcgt ctacggaagc ggcgggagcg cggatgtcct
      901 gtcgcagtgg acctactcct atcccgaccc gatccgcatc ggagtggcga ccgacgaact
      961 gtttgccatg acgcgcggcg ccgctcagcc gcagcaggtc atgaagatga cccaaatcat
     1021 ctggtaccgc tcgcagacgg caccgatcgc caagggggac gaggtcagaa aagcgcccgc
     1081 ctcgccgtgg gaggacaccg atcccgacgc ggcattcccg actattgcgc ccatgcatct
     1141 gcgcgaagcg ttctggacca agctggccag accggtgcgc ggcatcatgt atcacggctg
     1201 gggatcgctg gtgccgcaga gcggtgaggg ctcctaccgc tacacccatc cgcagacaca
     1261 gcatgagttg cgccgcctga tcaaagaggt cgtggagccg ctggggccca ccttgctgca
     1321 agtccccgac cggcccagcg atgtcgcgtt tctggagagc ttcgcgtcgc agatgttcgc
     1381 acggcgcgga acttacggct ggggcggcgg gtggaccggc gacgcctatc agatgctgct
     1441 gtgggcgcat cttcagcctg agatcatcta cgatgaaacg gtgaaggcgc atggactggg
     1501 caagtaccgt gtgctggtca tgatggactg cgatgtgttg acagccggcg tggtggctgc
     1561 ggttaaggca tttcaggcgc ggggcggggt ggtggtgggg gatgaacgcc tctgcccggc
     1621 tatccgcgct gacatcacga tcgcgcatta cacgcggacg cgcaaagcgg ataaagaccg
     1681 cgccgctttg atcgctaaat ctgccgagct gcgtacggcg atcgacacgc gttaccagcg
     1741 ttattgcgat gcctcaacgc cggacgtcgt gccccgctgc cgttcatacg gctccaccga
     1801 ttatctcttc gccgtcaacg accggcgtga gttcggcgat tacgtcgggc agcatggtct
     1861 ggtgatggag aatggcttgc cggcagaggc cgtgctgtcg ttggcacgcg cctcgggtca
     1921 tgtgtacgac ctgacagaag ggcgcacctt ggaagcgcat gtgcaggacg ggcggctaac
     1981 tgttgcgcgc gtcttgggtc cttgcgacgg ctgtgtgctg atggtcacgg aacgcgccat
     2041 tgatgcagtg cgtatcgacg tcgcgcaaga agcgcgcctc ggcagccgtc tgccctgcac
     2101 attttccgtg atggatcgag agggaaagcc gatcgatgcc gtggtgccgc tgcatgtcga
     2161 gattctcgat ccggccggcg cgcaagcgga gtggagcggg cattacggtg ccgaacgcgg
     2221 gcaactcgcg ctcacgctag atctggcctc gaatgaccgg cgcggcctgt ggcaggtccg
     2281 tgtgcgagag ctggcttcgc gccgctgcgc cacaacttat tttaggatag tgaataacga
     2341 aaccggaaac caacgagcgg tatgcctcat tcagagccct atctttgtgc gcccggatgg
     2401 cgtggggata tcggccttca tcccctccgc tccgggaatg ggctttacct cgtgcgtttt
     2461 ccagccgtcc gatgtgggct taacccccat cgcctcggaa acgaaaatga acgcgggcca
     2521 cgcgctccat gcgtggcaaa ggcttttcgc ccacggccgc ccgtaaaaac gatatcgctc
     2581 atcgcctttc tgtttggcgt taaacccttc ccagaaactc gtcgcgcccg catcaagcat
     2641 cgcgccgaac acggtttcca tgcccgtgaa aaactcctta tggtgtccgg tgcgcgccaa
     2701 ggccacaagt tcccatccga acacgtaagg cgtgcctacc gccggcaagt catctttggc
     2761 gagttcgtcg ccgatcgcct gcgcccgttc attctgggcc acgccgaaga tcaccgcgta
     2821 aatattggcc tgacgcgcaa accccgaatt cttctcaaac ggatcgacgc ggtaaagttt
     2881 gcgcccttcg tcccacgcga gacggttgag gttcgctttc actttgtcgc aaagcgcacg
     2941 gtactcagcg gcacgcgggt cgtttatgcg cccggcgagc ttcgccgccg cgtcaagtgc
     3001 tcctgtccat atcatcccta gtgcggtagc ggaccttgga ccagcccaat cgatgaacac
     3061 ccagtcgagc cctcttgcga caaagccgtt ttcatcggtg cgggaaatca ggtcttcgat
     3121 gcgttgcttg atccgccacc aacgcgcctc cagaaattcg cggtccgcga aataaagctg
     3181 atgcagatcg tgcgcgatga tcagccacat cgaataggtc acgatgccgt tcacgtccgt
     3241 tgtgtatgcg tccagcacag aaagcgagcg ccgggagatt tccgggtcac ccagaacata
     3301 cgcattcgcc atcagcgaca ccgtgagatc acctccccac ggcagacggt cgcgtttgat
     3361 gccgtcgatc aaaaagtcgt ggcagcaaag cttgagcgtg tggaccccca cctcgcgcat
     3421 tttctcgtga cgtttatcct tcgcggaaaa cgttcctttt tcctccatgc ccggccacca
     3481 cagcgggatc accttgacct tttcggcttt tccctcaaag cgaaaatatc tgagcgcgag
     3541 cgcggacctg gatcgccacg tgtcgttggc gaccaacacc atgtctgtcg cctgctctga
     3601 atcgaccggg accgtattcc tcatttccgg cacggattcg ccgacaaaaa gtttcggcgg
     3661 cttttcccca cttgtctgca cataggcaag caattccctt tgcgtgtcgt aataatcccc
     3721 gattttcttc aaatcccgat agtcgtgatc ttcttcaagc caccttaaat gcggcggcac
     3781 ctcgccgccg gaataacgcg cggcagcgcg ccacgtgtcg tcccgttgcg gaccggacac
     3841 cctcgcctcc caacccttga gcggactgcg caatgccggc gtgtcgagat accgtgcgct
     3901 caccgagaca cggagcatgc cggtcttttc gtcgaagttt tcgctgaacg cgcgtccgtg
     3961 cgtgaaatat tcgcgcgttt cgcctggctt gaaacgacgc cggaactcta cggctgccgc
     4021 gtacgggggc acgctgcccg gatatcccac atacatcccg gcggcctgcg cattccgttt
     4081 cagttcttcg agccggtaca actccatgct catctgcgga taataccagg cgtcacaccc
     4141 accgcctccg cgcggagaac tccccgcgtc cgcaccgtaa agcgcgctgg aacattgcgc
     4201 agaaatgcac acgcccgcca cccaaaccga caccgccgca accgttaacc tgcccatcgt
     4261 ctttcccctt aaacaaacat aagcgagcgc cgtgacgccg cagtcagacc cgcatcgacc
     4321 tgaccgcccg cttgaccgcc atcggctccg ggaccgatcc gtccgccatc gcgttatcca
     4381 tgcggtaaga ttgcaacttt gcagcggcat ggcaagcttg aagagttttg caacaatttt
     4441 gcttgccgca aaccttgtca atcccatacc ctagtctggc aagttaatac cttgtatagt
     4501 atagtcatcg gcacgacgat ggggttgcgg tcgagtgatc gcggaaagga atcggggtta
     4561 aatgcgtctt gatgcgattg tgaggttgct gttgacggtt tcgttcgttg cgcacgtcag
     4621 gtgtttcgcc ggaccggtcg tgacgccgtt catggaaaga acgaggccgg gggcggtaag
     4681 acccgagggc tggcttttgg acatggcgcg gaaagcgcgc gacggataca ccggtcacat
     4741 ggacgcggtg gacgttcagt tctcacgctc ttggagaaat gaaaccaggc cgcgcggaaa
     4801 ggatctcctc tgggtatccg atcccggagc ctggagttgt gagggcgggg cgtactggtt
     4861 tgacgggctg gtgaagctcg cgtatcagtt ggatgatccg tcgttgaagg ctctcgcatc
     4921 caatcgtctg gataccgttc tctcggagat gaacccgcat gccatcggat tttgctggtg
     4981 gctggatcgc aacgatcccc agcaggtttt ggagatgaaa atttcggatg cgtggttgat
     5041 ctgggtgacg gggatgtttg cgcgtccggt ttgcgcttgg cacgaggtca gcggggatgc
     5101 ccgggcgctc aaggcgcttg acttcgcgct tgacgaacac gctttttcct acggcaagtt
     5161 tcctacgttg ccctccggtg catatgccgg ttatcaggtg acgcggagcg agcgtattgc
     5221 ccgggcgctc gatgaattct ggaaaaaggc gcttcaccag cccggtcagc ttccggcggt
     5281 tttcagccaa tatttgacgc cgccccccgc gcatttggag gagacgctaa acatccgtcg
     5341 ccttcacgag cgtttactga agatccctac gcgtcacggt gtcatcgcct cggaggccct
     5401 gctgtcgatt ttcgacggat acctatggac cggcaatacc aatctcctga acgcggtccg
     5461 cggctggtac gcctttttcg accggcacat gcggcaaccc tacggtgtca ccacgatgga
     5521 cgaggagtgg gggcatcccg ggccggaacg gggtacggag acctgcgtcg tggcggcaga
     5581 gggctggacg cgtatcaacc ttctggcggc actgggcgaa ggatgttggg gcgatgacgt
     5641 cgaacgaaat ttctttaacg ccggacggaa ctgcatgacg gaggattacc gtcggcacgt
     5701 ctacctgcaa caaccgaacc gccttacggc aagcgatgtc acgcgctgtt cgtttacggg
     5761 tgcccgcggc gaccggctgg cgcgttacga cacgaaacat tggccgcttt gctgtacggc
     5821 ggcgctcaac cgtattctcc ccaactacgt cgcgtcgatg tggatgctgc ctgcggacgg
     5881 cggggtcgcg gcgacacttt acggcccgtg ccggtttgcc gcggaactgt caagcggctc
     5941 gtttgaggtg gaggaacaca cagactatcc gtacagcgat acgatcacct tcaagatcgt
     6001 caaatcaccg aatgcggcgt tcccgtttca tgtgcgattt cccggatggt gcgccaatcc
     6061 agaactgcaa gtcaacggca gacaaacggt gatcgccgca cagaacgggt tcgcggcaat
     6121 cacgcgcatc tggcgtgctg gcgacgtggt gacgttgagg ctgccgatga aaccgacggt
     6181 ggagaagatg ttcaactaca acgagccgcg aggacgcgag tacgccagcg tgtcggtcgg
     6241 gccaatcctc tatgcgttgc cgatctcttc aaaggacgac aacaatcccg aaggtgatgt
     6301 gcacgtccca ttggtctcgg ccggtttcga tgccgcgtcg gtcagggttg gcgatgcgat
     6361 gcgcatgttg acgatcccgg attcgaaggg aaacagcctc aagcttgtgc catatgggga
     6421 cgcgaagctg cgcatctcga tgtttccggt tgacggcgat tgagtgcgcg aatctgggtc
     6481 tcggggttcg aaccatgaag cacatgaaac ttcatgaaac caaaacgacg tctctccgag
     6541 gcacccgcag gttggctgtt tgacttgtgt tgcgaatggt gtaaactgta aaattcaatc
     6601 tgcttaagag ggggaatgcg gaatgacaac cgcaacgctg gctgctggct ggcggggggg
     6661 taaaagagga aataaaaaca ggagcaaaga catgaaagag acaaacgtga ttcgtgttct
     6721 tccggcgctc ggctgcggat tgctcgcctt cgccgcttcg gcggtcgaaa taaacgtggc
     6781 gtcggtcacc gatctgcgcg cggcgatcgc cgatacggtg aacgtgccgg atggctcgac
     6841 catcgtcctg caggcgtccc cgacgcacta cgatgtctcg gccgacggcc tcgggccgat
     6901 caggctcacg cgctcgctga cgctgagggg ctcgacggag aatccgcgcg acacggtcct
     6961 cacgggcaac ggcatgacca atatcctcta tgtgggcggc accgatacgc tcgcctgcaa
     7021 cctcacggtg acgaacgggc tctcctatct ggtcgattcc cgcaagtggt accgcggcgg
     7081 cgggatccat gtgaacggca gctcggccgt tgtctcgaac tgcgtcgtga ccggctgcca
     7141 cttcgatacg cgtaccgact acctcacggg cacagccgtc gccgagatga ccgcgttcgg
     7201 cggtgcgggc gtttacgcga acggcagctc gctcgtcctc gacacactga tcgagaactg
     7261 ctggatgttc aacgatagga agcagaaggt cacgggcgcc ggcgccggac ttttcatcgg
     7321 tggcaaagcc tggggcaccg atacgccatc gcgcgcttat cgttgcgagg tgcggaactg
     7381 ttcgaccgag acaatgtcgg tcggcggcgg tacgggagca ctcgtgggcg gcggcctgta
     7441 tgtgagcgac gcgggcatcg tcgacaattg ccgcgtccac cacaactggg tccggccgac
     7501 ggatgacagc tcccgctgct atggcggggg gctctacgtg ggaaccggtg gaatcgtcag
     7561 caattcgctc gtttacctga acacatgcca aaaatgcgtg acgaacacga gcggcaacgc
     7621 ctccggcggc ggcgtggcga tcgagggcgc cggacttttc tgcacatcga ccgtttcgaa
     7681 caactattcg tacggcgatg ccggcggagt cttccacatc aactatccct cgggcggtct
     7741 gcgcgactcg ttgatcattg acaacgaagc cgtcggatcg ggcggcgccg gcacgggggg
     7801 gcctgcggcg acggggtgcg tcttccaccg gaacacctcc ggcggcggcg gaggaggatg
     7861 gctcacgggc ggttcggcgg gctggttctc gaactgcgtc ttcgaggcga atgcgacggg
     7921 aaagtggggc gaggggaagg ccgcgcgtgt ccagcgcggc ggttcgaccc cgttcgtgtt
     7981 cgaaagctgc tggttcgtca atcacacggt gacggacggc aaggcggtcc ttcagttcga
     8041 cggttcgcct cttccaacct accgcgcgca catccgcaac tgcgtcttcg tcgggaacac
     8101 ggggtcgtgc ccgttctatt cgtcttacac cgccaccggg ccggagatcg acaactgcac
     8161 ctttgtcgcc tcggacacgg aatactacgc catcggagcg gggagtaagc gcgagacgaa
     8221 tgtcgtcgtc cgcaacacgc ttttccacgg ttacaagaac ggcggcctcc cgccggcctt
     8281 cgcgaccgac aagccggagc atgtctcgaa ctgctacgat caggtcgcga cgacgctccc
     8341 gaaggacggg gacggcaaca tcctcaacgg caacctcgcg ggaacgatcg tcggcgagcc
     8401 ggggttccgg aatctcgctg agacgaacct gacgctccgc ggggcgtcgc cgctccgggg
     8461 gaaggggctc gtcctcgact ggatgaagac ccaggacggt tcggctgact ggacggcgtg
     8521 cgatgcgggt gcgggcacct atatgatcga aaaggtcgca gactacggtg tcgcggtcag
     8581 attcggccga cgttacccgc gctacacggg cggcgcggac gctcccgaca tcggcgcgtt
     8641 cgagcattac ggtacggccg acgtcatgta tatcaagtag gaatcgggaa acagggacgc
     8701 ggaatggggt gaagagaagt ctggcgatgg ggctggcgct cgcggctgtt ctgccgtgcg
     8761 tcgaagcggg gatgaaaacg attctcgacg tgccggtcgc gggcgtcacg aacacggcgt
     8821 atctgacggg catggcctcc cgcccgtggt ggaacgcggc ctggaagcgc cgcgcgccga
     8881 ttctcgtctc ttcccatgcg gatgtcccgg agccgaaggc ggtcgtcgat tgcgtggtcg
     8941 acttcggcga gaaggtgaac ccggacgaag tgcgcgtggt gacgccttgg gagacggtcg
     9001 tgccgtgcgt ttgcgagaag ggcaaagcgg aaggtcaagg ccaagaacct gcggccgtca
     9061 ggctgctttt ccagacgccg ctcaggatcc acgagaacaa gccgttcctc gtctactggg
     9121 gcaatccggc ggcgaagcgc gagacaccgg taacggcgct gatgatgtcc gagactgcga
     9181 acgaggtgcg cattctgaat ggcgtgctgg atgtcacgat cgacaagaag catgtgacgg
     9241 gcggattcct caggaagctg cgcatcatgg gcacggacgc ctcgaatgag ctgctcgagc
     9301 gtgcgaccca gtgggcgtgg aacgggcttg agatgacgtt gggcggcgcg acggactggt
     9361 ccgacggcgt cgtcaccgag gacaacgcct tcgtgaagcg cgtccgcttc gaggggacga
     9421 acggcgcggt cacgctttcg gtctacgctg accagccgcg cgtcgactgg gcgtacgcgc
     9481 tgaagaaggg cgaccgctgc gaactcaacg tcgcctgggc ggtgggcggc ggcgtcgcct
     9541 acgacaagtt cgtctatccg ggcgcgaccg gcaggctcct cacgttcgat gctgcgctgg
     9601 acagcccgac cgactcgatc ccgcagcccc gcttcgagat ggcgccgtgg ttcggcgaag
     9661 gttggtacgc gatcgcctcg aagcggtcgc ccagcgtctg cgggcttctc ttcgaccgtc
     9721 ccgcgctgca ggggctctgg tataacggca gcggacaggc cttcggcgaa tggacgcgcc
     9781 ttacctttgc gcacaaggcg cggaaggacg cgccggtgac cggttcgggt tcgctcgtgg
     9841 cgcttccggg ccaggcgctc gacgtgcggc gcgaatacct gcggcggaaa acgccgctga
     9901 cggtgtccgt cggtgcggcg cagccgtacg cggaatatcc gtacgtgcgg ccgcgcctcg
     9961 accgcgactt catcgcggat atcaactgtg gcaaggattc gggccacggc tggcggtcgg
    10021 gcaagccgct gccggatccg gcgtgggcgt cgaacatcgt cgaccacctg cggtcgtacg
    10081 gggtgacggg tatccgcgtc ggcgggtact tttggtggga catgccgctg acggagaagc
    10141 tctaccgcga cctcgagcgg caggcgaacc gtccgctcgt cgcaccggac tgccgcaagc
    10201 gtttcacggg ctgggatccg gatcagtatc gcggcacgga cttcctcgag atggcgaacg
    10261 ccgcgcacgc gaagggactc gtcgtcaagc agtgcggtga cttcatcccc ggtatgtacg
    10321 ggcgcgagtt cgacaccaag ttcgacgccg atatccagag gacggcgctc gatctgcacc
    10381 tccggatggc cgaggtcggc ggcgtggacg tcatctacaa caacttcgag ggccaggagc
    10441 aggtcatcct gccccaggac gtgaagcgcg agaagcacaa cgactactgg aactggaagg
    10501 acccagacga attcttcgcc tggcaggaca aggcccgcgt gctcgtcaag gcgttctacg
    10561 acgcggcgaa gaaggcgcgg ccggacctcc cggttctcgt ctacaattcg gagaacggcg
    10621 aactgagccg tgagatcttc atgtccgagc agatcggcag tttcgacacg gtcgtcgtcg
    10681 agatgctctc ggggctcgac ttctcgcgct gcaagcacgt cgccaagcgc atgcgtgcgc
    10741 tcttcgacaa cgaagcgggg cggacggtcc atcaccacta ttacttcatg aagccggacg
    10801 cgatgcaccg catcaaggag atcgagctgc cgttcatatg cggcgtgaac ggcttcagcc
    10861 acgagaacca gagctacgag aacttctcgc gcgaacagag cgagatcgcg gccgacttct
    10921 accgcttcgc cgagtatacg cgcctcggcg agaaggccgc gcggatggcg cccgtccgtt
    10981 acctcggcgt cttccgcgat tccgtctgct accgcgagga catccgcaag ggccgtcacg
    11041 gacagccaaa agaccggccc tacttcaacc gtgccgagca ggatggccgc tgccgcgcac
    11101 tgggcgagat ccgcaacctc gacttcgact ttgtgatgaa cccgttcttc acggcgaagg
    11161 cgctggcgaa gtaccggatc gtctacgtgc cggaggacga cgtcttctcg gacgcgctcg
    11221 ccaaggaact tgtcgcctac gtgaaggctg gcggaacggc cattctcgaa gcgaaaaccg
    11281 ccgggaacac cgtcatcaag tcgcttggcc tcaaggacgg cgtgatcacg ccgctcggca
    11341 agggtaagat cctctggtat gagaaagtcc ggacggacgg acttgcgaaa cgggatgaga
    11401 agacgattcg cgaggttgcc gccgccatcg aggctctggc cgggccgatg ccgcaccgca
    11461 tagacggcgc ttcgtcgctc gacggtgtgt tgcagtccgg ttccgacgga atgctgctcg
    11521 gtgtccacaa tgcgggcgac aaggccgacg cgggacgggt gacgatcgcg aaagcgttga
    11581 ggtcgggacc ttctctctac gtcctcgacg tgaagacggg tgtgcgcttc ccctacacga
    11641 acggtttcga catcgcgatc gggccgcgac agtgcggctt ctacctgatc ggcgatgaaa
    11701 cgttcacggc ggttcccgaa acgaagacgg ccgtctggaa cggccctgcg gttgccgccg
    11761 cgcgtccgaa cggaatcccg gcccaggtga acgatgaccc ttcgttcgtc ccggcgaagg
    11821 ctgtcgaatt cacgcgcggc gatgggaagg gaaacccgac cgcgatccgc cgttcgcgcg
    11881 aggcgatgat cgatgtgacg cccgtgacgc cggccgactg ctatccgccc gtcctcgccc
    11941 gggccctctc aacagcgacc tacatccatt tctcctcgct cggcacgctt tccgcagatg
    12001 aaaagaagac cgtcgacgac gtcttcgtca gttgcgccga cgagctgaaa gcgctcctga
    12061 agcgcggcgg gacgatcctc ttcgaccgct ccccggccgg accgcagacg cgcgcgtttc
    12121 tgaaggagat cggcgtcttc gacccgtacg cgcatcccgc gccgaagctg ggcgacgaga
    12181 acacggtctg gacatcggag acgaaccatc cgttcgccgg gaatctcgcc gtgaagaagg
    12241 gcggcttcgg ccggtttcag gccaccgagc gctcattcgc gaagtgggat gccgcgcgtc
    12301 aggtcgcccc cgtccggatg gtacgcaatc acgatgtggc cgtcttcgtc cttcaggaga
    12361 acgtcctcgg cacgggccgc gtcgtcttcc tcgagaacag gttcgcgttc tccgattggt
    12421 acgagaaccg cgcgttcgga gatgcgctgc tcggctggat cgttggccgg ccgatcgaag
    12481 aacacgctaa gaagtccttg caacgtgccg gcggcatcgg cgttgcttta tcggaatgac
    12541 agaaaggaag cggatgatga aacacatgtt gttgctgacg gcttgtgcgt tgacaggggc
    12601 tcgtcttctg ccagctttcg gtgatgtgac gcctgcggcc ttgccgggcg gctacaccga
    12661 gctgccgtat gtcgaatcga cttacaacgg cggccagtgg gtctatacgg actatgtgcc
    12721 ggaatggaat caccgtatcg tttgcaagtt ccgcgtcaat caggtcggca cttggaactc
    12781 gcccggtgtc ttctgcgcac gcggcacagc gaccaatgac aaaggttact gtctcagctt
    12841 cgggcagggc ggcaacaacc gcgtgaatct cccgggcgcc cttcagtgtt ggacgggcgg
    12901 ctgggccgcg ccgtcctccg cgccgttttt ctatcccgat cttctgaccg actacgaggt
    12961 cgacgtcaac gttgaactca agaagtggtg cgtcgatgac ggggaacgct ggaacacgcg
    13021 cgagcacact tcgttcacgg cggggagccc gctcgcgatc ttctgcaggc acgagctggg
    13081 caactcgctt tcgccttctt cgaccttcgg cgccaacaac tgcatggcgg gccgcatcta
    13141 ctacctgcgc gcgtacgacg cgaacggaac gctgatccac gaatttgtgc cggcgcgcga
    13201 tgacaacgcg atggtgccga cgaagtactt cggcttttat gatacggtgg acgccaaatt
    13261 ctggccgaac tgtggcgcga cggcgttcgc gaccgatcct tccgtaccgt ccaacgtgcg
    13321 tgtcgtcgac cgcgccgatt acgatacaga cgaggaggcg ggcacggcgc tcgtcgcgac
    13381 ggttgccgcc gcgttcccga ccgaccggat caaggtgacg ggcgggacga aggccgcgcc
    13441 gcacgtctac cggatcacct cgcccctcct gatggagatg acgaacaccc ggctcttcgg
    13501 cgactcggac gatgccgagg cgacggtgat cgacgcgggc ggaacatcgg cgggcgtcgt
    13561 tgtgaagggc tccggcatct ccgtttcggg gctgacggtg aggaacgcga ccaactacgt
    13621 gaacggaacg acgtactgcg gcggcggcct cgcgctcatc gcggagggtt cggtcgcgag
    13681 caacatgatc gtctgcggct gcgtcaacgg cggcgtggag aaggctctgc gcggcggcgg
    13741 ggtctatctt tacaagtcga cgctcgtcga cgcgctcgtg aaggattgcg tcatctcgaa
    13801 cgacatggac acaacgggca agacggcgtt ctacggcggc ggcgtctacg ccgagcgctc
    13861 gaccgtttcg cgccttgagg tcagcggctg cctcgcggtc aacaaccaga tgtgcgaatc
    13921 gatgtggtgg gcgaccggcg ctttccgtgg cggcggcctg atgctcaacg aatcgtcggc
    13981 gaccgactgc tacattcaca acaatatgat cacgaacggg ccgaagcaga cgtcgagctg
    14041 caacgtcagc catggcgctg gcgtcgcgct aatgggtgcg tcgacgcttt cccgctcgct
    14101 cgtcacgggc aattccgccc cgtgctctgc cgcaggcgtc aacttggagt cggcgtcggc
    14161 cgtcattgac tgcacgatct ccagcaatac ggtgttcttc gcctggtcgg gcgcgtatcc
    14221 gggtggcggc ctctacctgc gaaacgcgtc gcaacgggct gtcaactgcc ttctggaggg
    14281 caaccggaat ctggggacct actgggacgc gggcggcggc gcgggcgtga tcgaagcggg
    14341 ggtgttcctt ggcggcgtcg tgcgcggtaa cgggtcgtac aacacccacg gaggcggctt
    14401 cttcctgaac ggtgcatgcc tcgtctccaa cgtcgtcttc gacgcgaact ggtcaacgga
    14461 cagtagtgat cggtgcgggt ctggcgcgat cgagttccgc ggtggcggcg ctgtcgtccg
    14521 tgactgctgg tttgtccgca acatcggggg cgacaacgcc aacaacagct acggaacggt
    14581 cttctacggg tcctacgaca ccggcagcgt gttgcaaaat tgtttctttg caaataacac
    14641 gggcgcgtgc gtcctcggcg gctcgatcga gacgaacaag gccgactgtg tggcggaggc
    14701 ctgcacgttc gtgaagaacc atgtgtcgcg cggggcggtc tggtgcacgg cgctcgacta
    14761 ttcggttccg gggacggttc aaaagatggc cttcacgaac tgcgtcttct ggaagaacgg
    14821 cgagaacgag gacaagcccg gtattccgga caagttcaag aacgtctcga cgaaggtatc
    14881 gtactgctac gacgagatcg gcacgggcct cacgccgggc gactaccata acctgacggc
    14941 ggcgaacggc gagccgttct atgcggatgc ggaaaacggc gaatggcgca tcggcaggaa
    15001 agcgagcgtt ctggtcgaca acggcctgaa gttggactgg atgggcgacg gagcgaagga
    15061 cgggccgctc gacatgggcg acggaacctg gagcgaagcg ccggcggcga cgatttccgt
    15121 gcgcggcaaa acgatggacg tcggcgtgat cgtcacgcgc aacaatgcgc aaccgcgcat
    15181 tagcccgcag aacggtctgc ccgacatcgg ttgcgccgaa tatcaggcgg tgaacggatt
    15241 catcttgcgt gtgcactgat cccgggtggt ctcggtatca catcgcccgc aatggaaatc
    15301 ttcattgcgg gaaccaaagg attcatcatg agaagtttca acttcagtca tgccgttctt
    15361 gcgttgtcga cgattctgat cggggcgtcc gcgtccgcgc ttgacattct cgatgtgccg
    15421 gtcgcggcgg tctcgaacag ggtctacctc gccgacatgc cggagcgccc ctggtggaac
    15481 agggcctgga aacgccgtgc ggcgattctc gtctcgaaca tgtcggatgt ccgcgaggag
    15541 cgcgcgatga tcgatttcat cttcgacgtc agcgaggcgg ttgaagcgaa gtccgtccgc
    15601 gtggtgacgc cgtgggggac cgcggtgccg tgcgtcgccg aaccggtgcg tcagggcgag
    15661 ggcgcgctga tcacagcacg tagccgggac gtccccgccc tggtcgcgcc aaccgcgctc
    15721 cggctcctct tcgagacgtc gctccgcgaa cgcgagaaca ggccgttctt catctactgg
    15781 ggcaatcccg acgcgccgac gcaggggatc gactcgccga tgacgctctc gtgccgcgag
    15841 gacgaggtgc gtgtcaacaa cggcatcatc gaggtcgtct tcgacaacct ccgccgcacg
    15901 gacggcctca tgaagaagct gaaggtgaat gcctcgcagg caccgagcga actcctgtgg
    15961 cgcacgacgg gcttcgcctg gcggggcttt gagttctctg ccggaagggc cccgaaaccg
    16021 tggtcgaagg cggtgatcac cgccgacaac gccttcagga aaaccgtgac cttcacgaac
    16081 gaggacgccg tgctcgactt cacgttttac gcgggccagc cgcgtgtcga ctatgtctac
    16141 cgtctgaagg acggtctcca caacgcgcgc atcgatgttt cgtgggcgtg cggcggcgac
    16201 tgtggccacg atgacttcac ctaccgtggc ctttcgggcc agctcctcac gttccgcgcg
    16261 gcgctcgatc atgtgacgga ctgtatgccg cagcccgaat actaccgctt cccgtggctc
    16321 tcggaaggct ggtacgcgat cactgaccgt cggaatcgcg atgtcgtcgg tatggtcttc
    16381 gaccgccggg ctctgggcgg cgtctcctac atcgggcagg ggcagtcgag cggcgagtat
    16441 gtttcgctga acttcaacca cgcgccgaag gggaagacgc ccgtcacggg atcgggcgcg
    16501 ctcgtcgcga cagtcggcga ggcgcggacg gtcgccaccc tctacgcctg gctcacgcat
    16561 ccgccgaagg tcttcgtcgc gaaagccgag tcgtaccgag agatccccgt gaaggttccg
    16621 gttctggacc gtgacttctg cgtcaacgtc aacgtcggcg gctggcgttc gtccacgccg
    16681 cttcccggcg acgagtgggc gccgaacatc atcgcgcaca tccgcgcact tggcgcgaac
    16741 acgatccttc tggggcagtt gacggactac agttggacgt cgctcccgat ctcgaaggac
    16801 ctcttcgacc gactctgcgc acacaagaag gcgcgcaacc cgtcatggaa ggctcccgaa
    16861 tggaagggcg gttacgacgg gcggcttctg aagcgcatct gcgccgaggc ccactcgaac
    16921 ggactggccg tctgcacatg gcacgacgcg ttgccgggct tcagttacgg cggcaagttc
    16981 gacgccgagg agcaggccct ctgccgcgag atgcaggccc tctacccgat gtgcggcgtc
    17041 gattcgacct tcaacgcgct cggcggcggc gaaggcgtcg agttcccgga cgacgacctc
    17101 aagaagttcg gcagggccta ttggaagtgg ccggaccccg agccgttctt cgcctcgcgc
    17161 cgtctgagcg gcgaatacat gaaggcgttc tacgagtatt cgcacaaggt caatcccggc
    17221 acgaaggtga tggtcttcaa ctccgagaac agcgagcttg aacgcgacat gtgcatgggt
    17281 tactaccccg gcgtcatgga cacgctcttc tgtgaattcg tcgcgggcat gaatctctcg
    17341 aagatcaagc acacggccaa acgcctgagg ggctatttcg acaaccgtga cggacacacg
    17401 attcacgccc actactacaa catgacaaag gactacgccc accgcatcgg tcagcttgag
    17461 atgccgttca tctgcgggat caacgggttc tcgcaggagt cgatgacgta cgaaaacttc
    17521 gaccgcgacc tcttcgagat cacggccgac ttcaaccgct tcgcgctcta cacgaagctc
    17581 gggccgaagg tcgcgaagat ggccccggtg aagaacctcg ccgtcctgcg cgacatggcc
    17641 tgcttcgagg aggactgccg caagtaccgt acgatgccgc gcgactggtg gcatgcctcg
    17701 cgccacgacg cgcgcgtcaa tgcgttcgcg gagatcttga gctacaacta cgatgtcgtc
    17761 atcaatccgc atttcaaggc cggctcgctc gctcgctaca aggccgtcta tctcaccgac
    17821 gatgaggttc tcccggaagc cctcgcggca gagctcgtcg cctacgtgaa ggcaggcggc
    17881 ggcgcggtga tcgaggggag tacgggtgaa agactgaacg ttgaggaggt gaaaggctgg
    17941 gaggacggga aggtcatcga actcggcaag ggccggatcg tctggacgaa ggaggttctc
    18001 accgatccga tgatgaaggg cgatgcgaag gcgatcgcgc gtgtgaaggc gctcgtcgcg
    18061 tccgtcggcg gcgtcgctcc gttcgagatt gcgtcgaagt ctctcgacgg catccttcag
    18121 gcaggtgaag aggggatgtt cctcggcgtt tacaacaagg gcaagaccga agacaggggg
    18181 aaggtgacgc tttacgtcgg cagtccgtcg tatgttctcg acgtgaagaa gggcgtgcgc
    18241 ttccccttca ccaatggctt cgagattgcg gttgggccac ggcagtgcgg cttctacctg
    18301 atcggcggcg acgcgttcac ggcgattcct gccgcgcagg aggctgcgtg gatgggggcg
    18361 gcgctcgcga gcgtccggcc ggcgggtcag aagccgccgg agaatcccga tcccgcgttc
    18421 cggccgtacg tggcggtcga gttcacgtcc gtccgcggcg agggcgcgaa ggcaaaggcg
    18481 gccggcatca cgcgctcgcg cctggcgggt atcgaacgcc ggcgcttcgt cacggacgag
    18541 gcgacgctct gccgcacact caaggtcgac gacctcgatg tgtggcgctc gaacaacacg
    18601 gatgtgaaga cctattcggc gcaggcattt gccgcagcgc tcaagacggc gcattacgtc
    18661 cacttcagcg ccaatccgaa ggactgcgac gcggcgttcg cggactgcgc cgaggaactg
    18721 aaggctcttc tccggcgcgg gggcggtctc ctcttcgaca ggaacggcac ggggcccgcc
    18781 gcgcgccggt tcctcgccga gatcggtgtc ttcgacccgt acccgacggc gaaacgcggc
    18841 atcggcgacg gtgcaccggc gtgggacgag acgttcccga cgaaccaccc gtactacgcg
    18901 acgaacgaga caaagccgcg ctatggcgtc cagcggctcg aggccgacgg cgggcagtat
    18961 tcgctcgcct tcacgaagtg ggatccgcaa acgcagcttg cgccgttccg cacggcgcgg
    19021 gatccgtcgg cggccgaact cgtcattcag gacaaggttt tgggcgccgg caaggtcgtc
    19081 ttcaaccaga acagccgctc cttcaacgac tggtacgaga cgaagaccta cggcaacgtg
    19141 atcctctcgt ggttcatcgg tatgccggtt gacgagcacg cgaagaaggc gcggttcttc
    19201 gtcggcggca tcggagagag cgtgaaatga cgaaaaggaa aaggatgatg aagggcaggg
    19261 tgaaacagtt ggtgatcgct gccgttcttg ccgccggttc ggttgaggcg aagaccgcgt
    19321 tggatgtgcc ggtgtcgggt gtgacgaaca agatgtatct gtcaagcgcg gcgacgcgtc
    19381 cgtggtggaa cacggactgg gcgcgccggg ctccgctcct tgtctcgtcc atggcggaca
    19441 tccagtcggg tgccggatgg gtcgactgcg tggtcgactt cggcgagacg gtgaatcccg
    19501 acgagattcg cctcgtcacg atgtgggaga cggaaattcc gtgtgtggca gagaaggttg
    19561 atggaggacg ggtcaggctt atcttccagc tgccgctcag gattcgtgaa aacaaaccac
    19621 tgctcgtcta ctggggaaat ccgaaggcag agaagccgac gttcatcgaa acgatgaacg
    19681 tgacgatgga cggggcgacg gcgcggctgg cgaacgggcg cgtggagctg acgatcgacc
    19741 gtcggcagga ggcaggcaat acgatcaagg ggtttcgtct tgtcggctcg cagacgggcg
    19801 atctcgtgac gctgcgtgcg acgggcgctc cgtggaaggg ctttaccttt gcgacgggac
    19861 gttcgaacgt cgtctggaaa acgtgccgcg tgacagcgga caatccgttc aagaagcagg
    19921 tcacgctcga cgctcccgag gcgacgcttg tctggacgat gtatcccggc cagccgcgtg
    19981 cggactacgc ctatacgctc aagcgggatc cgagcgcccg cattgtggtc gcctggtgcc
    20041 ctggcggcgg cgcggcctgg gacgatttct actatacggg cctcaccggc gcgcgtctca
    20101 cgttccgcgg ctcgctcgac gaggtgacgg actcgggcgg ctttccccat aacgaccatc
    20161 tcgagaaatg gatgaaggag ggctggtacg cgatctgcga ccgcaagaac ggcgacacgg
    20221 cgggcatggt ctacgaccgt gcgtcgctgg cgcgcgtctc ttacggcgcg gctcattata
    20281 acggtggcga gcgcgtcagt ctcacgttcg agcatgcgtc caagaagggc gagagcctcc
    20341 gcggcgcggg ttcgggtgcg ctcgtcggca cattcggcgg tgccgaccag gtggcggcgg
    20401 aatacgcgcg gcttgtggcg ccgccgcagg tgtttcttgg caaggccgaa tcgtgccgta
    20461 cgattcccat ccgtctgcct gaccacacga aggacttcat gggcgagttc gtgacgggca
    20521 tgaaccctgg gaactgggaa catcttcgtc caggcacgaa ccccgactgg gccgacaatg
    20581 cggcgaatta tctgcggcga ttcggcgcga cggcgattcg tcttacagga tacggctggc
    20641 aggaactgtg cttgactgaa gacctctaca accgctgtaa aacgatcatc cgcgaagatc
    20701 cgaagatcgc cgatcacgtc aagaatcgtc cgatcgccga ctggtcggtc gaacggtatg
    20761 cgggcgtgaa tctccgcaag gtgtccgaag ccgcgcaccg caagggcctt ggcgtcagca
    20821 cctggacggg gaggggggcc gagatcttcg gcggtctcga ggcggacgtc accgagacgc
    20881 cgctttaccg tcagctcgac ttcgacctgc agaaccgcta tccgaagagc ggtgcgggtg
    20941 tcgtctacaa cagccagtgc agtttggaag gcccgtggat gccgaaggcg ctccgtcagg
    21001 aacgcggcaa cgagtactgg cgctgggaga agccggagga cttcttcaat gcgcaggacg
    21061 acttcatccg ctacatgaag gcgttttacg cgcagtcgaa gcgagagaat ccgaagacgc
    21121 ccgtcttcgt attcagcgcg gagaacggcg gcctgaaccg cgaactgccg cttgcggacc
    21181 tgatcggcgc gtttgatgcg gaatactgcg aacttgtgcc gggttacctt gcgagcgccc
    21241 agggcatcaa gcacagcgta cggcgcctgc ggtcgcattt cgacaacaat ccgcacacgg
    21301 ccttccacca tttctattac tacacgtccg accacggctg gcgcatctcg cagttggaag
    21361 tgccgttcgc cttcggcgtc aacggctttt cgcacgaggt gatgatgcac gatacgatcg
    21421 acgtcgaact gaacgagatc acgggcgact tctaccggtt tgcggaatac acgcgtcttg
    21481 gcgggaaggt gtcgaagatg gcgcccgtca agcaccttgc ggtactgcgc gacgcgcggc
    21541 tcttccgcga gaacgtcctg aacaagcgtc cgaacgagca ggagagtcgc ctgcgtggct
    21601 ttgccgcgct ccgtaactat ccgtgcgata tcgtggcgaa tccgtttttc acatgcgact
    21661 cgctcaagcg ctatacgatc gtctacgtgc ccgacaaccg tgttttcacc gacgaacagg
    21721 ctgcggaact gctcgcctac gtcgaagcgg gcggaaacgc cattgttgaa ggtgtgacag
    21781 gcgagaaggt caagggactg gtcgacggcg agatcacggt gctgggcaag ggcaagatcg
    21841 tctggtcgaa ggaggtgctg tccgaacagc tcgcgaagga cgatgcgtca aaggttcttc
    21901 cgcttctccg caagctcggc ggcgaggagc cgtattccgt caaggccggc ggctcgtgcg
    21961 acggcctctt gcaggcctcg ggtgaagggt ttttctatgc ggcctacaac gtcgccaaac
    22021 acccgagcaa gggaaggttt gtcatgaagg aagaagttcg gaagagcctc tttgttctga
    22081 acgtgcggac aggcgggcgc aagccgttcg acggttcgtt cgagtttgag gtcggggcgc
    22141 agcagtgcgg tttttggctg atcggtgacg aggccttcac ggctgttccc gaggcaaagg
    22201 cgtgtgtata tgacggcgct tcggcggttt cggcgaagcc cgcgccggtg aagctgccgc
    22261 agcacgacgt tgagaaggca aaggcgttca aggagcttga gatcgtcgag gtgctctcgc
    22321 tcaatccggg gttcctcggc tatcagacgg gcggcgagat cgggcgtatc gaccgtcacg
    22381 agcggctcaa gctcaagctg acacggttct atccagacga aaaggcggtt gcggaggtcg
    22441 tcaagcctga ggaactcgat acgtggaaac agatcaacaa gggactctac aatccgtttg
    22501 atgaaacggc gttcgcgaag gcgctcaaga cggcaaaggt gctttacttg cgtggcagcg
    22561 cagcgcagga tctgccgttt gtccgctgtc cgcaagcggt gaaggacttc cttgccggtg
    22621 gcggcggcat catctttgac tgtctcgatg cgaacacgaa cgtgacggcc ttcctgaaat
    22681 cggtcggcgt ggaggacccg atggcggcgg gacggttcgt caagtcgtgc ggacagcggg
    22741 ccgtcaaggc gccggggctc gagcatccga tcgtgcgcga tacgaacgag cggattaacg
    22801 attttaaccg cgagggcttt tgccgctatc atctgacggg cggcggttgg aagggctgga
    22861 gcaaggatca gtttgcgccc gtgcgcgcat ttgaggatcc ggaacagatc gcgacggtga
    22921 tcgtgcagga gaacgtctgc ggcaagggcc gcgttgtctt ctcccgcaac tggaacgcct
    22981 ggtggagtta tcggagttcg atgtgcgacg ggcttttgtc cacgaacatc ctctcttggc
    23041 tgttcgacta cgacgtaaac gcatacgaga gcgacttaca ggtcaagaac ggcggtccgg
    23101 gagaggtggt cggggagcag tagatatcca ggttgaaaca aaaattatgt cagtcgagtt
    23161 ccgtaacgtc aggcgctgtc cgctgtggcg gcgccgccca aagccgccgc cgccagtgca
    23221 accgttgcaa aacctcctgc ctcaccatcg cgggtcgaat gtcgcgggtc gcggggcgac
    23281 gatcccgatc ccgaccccga tttggatgaa accctgaaac acatgaaact tcatgaaaat
    23341 caggaattcc ctctggcgtt ccaaaaaa
//
LOCUS       DAOSRB010000018        23032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2405_length_23032_cov_6.653044, whole genome shotgun sequence.
ACCESSION   DAOSRB010000018 DAOSRB010000000
VERSION     DAOSRB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23032
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_23032_cov_6.653044"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1912)
                     /locus_tag="PLZ74_02020"
     CDS             complement(143..1912)
                     /locus_tag="PLZ74_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOR97129.1"
                     /translation="MMKTMLALGWGFGVVILACAGERVALWPEGKIPDFQPRQIAATT
                     QEVKEPGFKPAERVMPYLDWYEAPAQKNGACMLLISGGGYQSCCDGVWIDRVAKKLID
                     LGFVCVSLTYRTPRPQGLPIYQSGWQDGQRAVRLVRNEAQKRGFDPEKIGAFGFSAGS
                     HLTVLLATSALTPAYGPVDDLDRLPCHINWAVPIYTAYALTDGLTGPNTRDGDAIDVK
                     LTDVFKFDAKTCSMALFHGGMDAYSPNGSTQIYRQLRRMKIPAEIHLFADRPHGFMGN
                     PDKGEQGTAYDNWLDRIAEYLRQMNFDGRLGEEVDLMTRYPNDDARDGYRKEPVWPEG
                     RMPDVQTNQGLPYLEWHMPKERKTKAIQIIYSGGGYGGNSPDGFEVAPTRRFLNEKGM
                     TVVTMKYRTPRPLGGLAKHTTAWQDLQRAVRVVRSQAAALGLDPDRIGIMGSSAGGHL
                     TLMGATSSKRRSYWPIDDLDKIPCNVQWAVAIYPAYALTDGSNAPNAKGGNEDDSRPV
                     PEFSFDLATCPILFIHGDADSWAPMNSVKCWEQLRRMGIQSDLHTLATRNHCFQRKAS
                     PGTGSYTWMGRIWEFMNHKGFNK"
     gene            complement(1927..2973)
                     /locus_tag="PLZ74_02025"
     CDS             complement(1927..2973)
                     /locus_tag="PLZ74_02025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR97130.1"
                     /translation="MRPLIRLNVSLLLVLVCCWSGCGKKSEPAAEVESAANSAPTAGV
                     TSPDYVVAKVNGTPLTWEEMDRRAMGYLKDDVEVNHLVIPSNRLEEAREHFRRRAINA
                     FVFKTVMLDEALKRKIAVTEADRQAGLRALQNTLKSRNWTTNDFFERGPQPPRMMRRE
                     FEDGLIIDKLLKREVRDKIRVEEAELTEAIGVLHETNQLRRARLETIRKQLLDGADFA
                     DMARNVSECPSAKDGGDLGEFARGKKVKPFEDAAFSQKIGEIGPVIETRFGYHIIKTT
                     SRMPAKVATSTTPAVPETVRASHILIKSVPVNRRRLSDTILRRKYNTGVETFFAELKK
                     KAEITCYLYQDMTF"
     gene            complement(2998..5211)
                     /gene="pflB"
                     /locus_tag="PLZ74_02030"
     CDS             complement(2998..5211)
                     /gene="pflB"
                     /locus_tag="PLZ74_02030"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOR97131.1"
                     /translation="MSLSSDIWRININVRDHIISHYTPYDGDDAFLAPVTGRTRKLWD
                     QVRGLMEEERARNGIYDIDNSRISTITSHAPGYIDRELEQIVGIQTDTPLQRAIMPFG
                     GIRMVESSLKAYGRELPQAIQDVFRFRKTHNDGVFDAYSDEMKLARHNGIITGLPDAY
                     GRGRIIGDYRRVPLYGLDFLIAHKEAAKARHVFDAMDMEAIQLREEISEQIRALQELK
                     AMAASYGYDIAQPARDTREAIQWLYFAYLAAVKEQNGAAMSIGRISTFLDIYAERDLA
                     AGRYTEAEIQEFVDHFVMKLRMVRFLRTPNYDALFSGDPTWITESLAGIGVDGRHMVT
                     KMTYRFMHTLVNLGPAPEPNMTVLWSPRLPEPFKRFCARLSIATSSIQYENDELMREK
                     FGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYCINGGKDEITGQQVGPEMAPVTS
                     DVLDYEETVRKFDQICRWLSRLYINTLNVIHSMHDKYCYERLQMALHDEDVFRTAACG
                     IAGLSVVADSFSAIKHAKVRPIRDARGLAVDFEIEGEYPTFGNNDPRVDEIAVEVIKT
                     FMGRLSKHRTYRHAKPTLSILTITSNVVYGKTTGSTPDGRKKGEPFAPGANPMHGRDS
                     RGCIASMKSVAKLPYDHSEDGISFTFSIVPNALGRTPGERVENLVGLLDGYFSEKGHH
                     INVNVLEREMLLDAMEHPENYPQLTIRVSGYAVNFIKLTREQQLDVIHRTFHKTF"
     gene            complement(5262..5338)
                     /locus_tag="PLZ74_02035"
     tRNA            complement(5262..5338)
                     /locus_tag="PLZ74_02035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5302..5304),aa:Arg,seq:ccg)
     gene            complement(5440..7101)
                     /locus_tag="PLZ74_02040"
     CDS             complement(5440..7101)
                     /locus_tag="PLZ74_02040"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOR97132.1"
                     /translation="MFIYHFNRLIRSRILWGFFVIVIAVAFVAVDSCFQRPQDRMAAG
                     KLNGKTVTSEEFDRVVQTIRGFGQNRDNTTPASVVDRRAWEQLAARQVARKNGLAASR
                     DEIRAVLREAPGFQGPNGFDLNRYRAVLAEQGLTPTLYENMVSHQLAIMKNAALVETA
                     AWVAPMELEDELAAMTDIFTVQVAAVSNRFAEAAMPLTDADYRKYYEENKDSFALPDR
                     MSVRYVMIPVSNYLAAVSVPEDDLLEYYDSHANTYTRTTTNNTTEPIPFAEVRTNILA
                     ELMLEEARYCAETSVTFTVYGKLTAAGSNALDQISMQQRLPVKRSPLFGAEEALFWVE
                     DSKAFADAAFELDPERADSRFGIVKGQQFIYVIEPLERSEAHTPTYEAVLEELKPRAQ
                     AKARTEKFDNVAKTLREDLLKRLGEGKSFAEAAREKALNVTTALTYTVSDIQNQKFEN
                     SFSIAYGAMTLRKGELSEIIPVSAARALLVYVQDRQPGDALAAEMMRAQIRNAIARRR
                     NANLFDAWLSWNLSCQDFQPTRPLDDEEDGETEKITAPAEDDDAG"
     gene            complement(7202..8200)
                     /locus_tag="PLZ74_02045"
     CDS             complement(7202..8200)
                     /locus_tag="PLZ74_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97133.1"
                     /translation="MMDQTGLLRVGCCVMGLALLTALAQENAAPVAGEAVPAAAQESD
                     AAAVKVFDPLVRLMNVLGVCEVNIPGTEGFAPATNNKYYPLGTVFRIGMGGSALLVFS
                     AQESVKLTANTEVVVTAPEKNPEARVLRFSGGAMKTTLRDNLQEGLFAIETPSTTCRN
                     VAGRGEYTLVCDGTLETFQAATITGSALVEGAQYHIPALRAANTVNIQNAPDRTLTRL
                     TSVSGDFPIVLENGTETPVTFGMTPKAVVKIWRENAPVGGRPIISTLVVSPTGIARHR
                     FVYAEGRPDLTTGELIVPAEPEPKEEALPVLLDGVAQDSAAPAVPATPAADGQKEP"
     gene            8440..8880
                     /locus_tag="PLZ74_02050"
     CDS             8440..8880
                     /locus_tag="PLZ74_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOR97134.1"
                     /translation="MKISTKGRYGLRILMDIALHQDAGAVTLNEIARRQGISVKYLWQ
                     VINPLKTAGLLSVTRGAKGGYMLARSPEQITVLDIVRILEGSVVLLDCLTNKKHCDQM
                     DICVARTVWMEAQNAMEDVLRGITLETVLRRCADATATANDYSI"
     gene            complement(8924..9712)
                     /locus_tag="PLZ74_02055"
     CDS             complement(8924..9712)
                     /locus_tag="PLZ74_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97135.1"
                     /translation="MSQKHARADESAKTDLKTIPENLPEELLPLYDWWRSKGPQSLIT
                     FGAVVLVCGAVWAFKYYRTAKIAAANQAFLQAHSLEDLEGVVAQYGSFKAGNAARLRL
                     AKAYFDAGKAQESLDLYELCLKKGAPQGFREIAELGRAHALEALNRLDDALTAYQEFE
                     RDRTDHFLQPQSVMGIARVLTLQGKKDEARKRLENLKAAKTGDATWEMAVAQLQGVVD
                     RYEPRAARSLFDAADQAAQSVPPALAVPGAVTQSAPAAVSAPEK"
     gene            9915..11183
                     /gene="guaA"
                     /locus_tag="PLZ74_02060"
     CDS             9915..11183
                     /gene="guaA"
                     /locus_tag="PLZ74_02060"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOR97136.1"
                     /translation="MKQTHDTVVILDFGAINSQAAAKLIRQLNIYCEVLPCTAARERI
                     EALRPKALVLQRGAEFGANTLKRSPATDPAAWGVPVLDLSDLPCEIATFETFLLSQCG
                     LRQDWTVEAFIREAIDEIRGMVGSHRVLLAMSGGVDSSVCALLAHRAVGDRLTCVFVD
                     HGLMRKGESDEVCSVFRDRFGIPLRHVDAAERFLARLAGITDPEHKRKIIGEEFIRVF
                     EEEAAAAGEPDFLVQGTIYPDIIESGWDGGKPVKAHHNVGGLPDRIAFKGLVEPLKYL
                     FKDEVRQVALALGIPAAIAMRQPFPGPGLGVRCLGEVTREKLDILREADFLFRNAIVA
                     ADLETQANQYFAVLTDSRSVGVSQDARTYAYTVALRAVQTSDFMTAQFVKIPLDVLES
                     VSEQITREVPQVNRVVYDITNKPPSTIEWE"
     gene            11259..13703
                     /locus_tag="PLZ74_02065"
     CDS             11259..13703
                     /locus_tag="PLZ74_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOR97137.1"
                     /translation="MQNFEKLGSFYLGKEYDLAAGARTDNLLLYDAKDLTTHAMIVGM
                     TGSGKTGLAISLLEEAAIDGIPAILIDPKGDLANLMLQFPDLTPDAFQPWVDPSEATR
                     KGVSLADLAAATAQTWRNGLAEWGQDPSRIRRLRDGAEFVIYTPGHASVRPLQILRSF
                     AAPPPALRSDDAALHDRVLTAVSGLLGLLGLDADPVQSRDAILLSNLFCHAWREGRDL
                     DLAALIRDVQKPPFARVGVLDLETFYPSAERMGLVMRLNNVLASPGFSAWMEGEPLDV
                     QRLLFSPTGKPRHAILYLAHLSDTERMFFVTLLLNEVTAWMRAQSGTGSLRALLYMDE
                     IFGYFPPTANPPSKTPMLTLLKQARAYGLGVVLATQNPVDLDYKGLSNCGTWFIGRLQ
                     TERDKKRVIEGLEGAAASSGAGFDRARTEQLLAGLGNRLFLMRNVHEEEPVLFQTRWA
                     LSYLRGPLTLPQLATLAPPRDTIPEAQPVTQTAALSGASFPTAATPLPPAAAHPLPPD
                     LPVFHLRPGASFSADLVYRPMAAGLVNMRFVDAKAGLDFWMNGIALAPFLDEGARAAW
                     EECEIRRGTSSALLDPQPLPTVAFETPPVHLPGSKQLASWAKDLESHLYQTHTFELLT
                     YPDLKLAAMPDESEGDFRARIILRLREQRDAELAKWKAAYAPKFQTLTDQIRRAEERV
                     AREKEQAGQQKFNTALAFGATLLGAFLGRGGAPVGTVGRATTAMRSAGRIRKESGDVA
                     RASENLETLRQRLSDMQEQFDRELSALQAPVAAEALSLVKRPVRPRKSDLSVGTVGLC
                     WVPWRKRPDGMFEPAG"
     gene            13772..14134
                     /locus_tag="PLZ74_02070"
     CDS             13772..14134
                     /locus_tag="PLZ74_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97138.1"
                     /translation="MSCSIPDTRTRLLLLRGVLRRFFLNLFRPAYVRDSLARRQGACN
                     RCGACCHLVANRCARLRVYPDGHSECWRYEGFRMPNCKTFPIDARDLAERDWIAPDTP
                     CGYFWPDAPVTPQPSRRS"
     gene            complement(14152..15291)
                     /locus_tag="PLZ74_02075"
     CDS             complement(14152..15291)
                     /locus_tag="PLZ74_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOR97139.1"
                     /translation="MNNPIWLMTSAFPKLTFAQVVKRAKAVGAQGLELCVFRRDGSRQ
                     DHVATHLNYETFTLEDARRVLETANKEGLRFSVGAYDNLLGGDPAVCEANRNHILKLI
                     RIAHLLGGDANDVCVGTFVGYNHELGKEDGGFAKNLECYARVFRPIVRYAEDLGVTLT
                     YENCPMEGWRPASTPTTYNNLPCTLAARKLMYALIPSPAHGETYDPSHDAWQHIDPVD
                     VVRNTDLNRLRRVHIKGTRNRTNSLAAIQWGRLYPMQQVDAKLAKRAGVCQPASEWDR
                     HHYDPKLPGFGGSDSIDWFAFLKTLMELGFDRPFVIENEADNSSHTGNAAATLQGFAA
                     TVMALAPAVWPLDAEKGWCFNAKRLRPLKSAPKKDVPVVTMQDLM"
     gene            complement(15408..20225)
                     /locus_tag="PLZ74_02080"
     CDS             complement(15408..20225)
                     /locus_tag="PLZ74_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOR97140.1"
                     /translation="MRSRLWVLLVPILVLMTCRGFSQSVWTGGGAGPQWSIGGNWQGG
                     AAPVPGPLTSLTFDGSVTQTVNDFQPGSFFSNLTFAASSAPFNLSGNPFILCGNLANN
                     STGRQRIAADVAWSGVSRTLDTGAGDVVLAGHLTSGAGNAAFTKNGSGRLILEGGANF
                     TAVDPVSKINGGSLIVASDAVFNRLDLQNNASLIITNNATLSGFTASYVFWQACTVDL
                     YSGRIVVGNSLLTAQDRTKTCVVNVRGGVFTAGGAPRWSNNGNTLLTLSGDGLFEWLA
                     TGQELAENGGSHVILNGGMFKFSADGAGEFDVGYRLSNPKRDGTITNRIDFNAGLFLT
                     GGFKHSAKTNLLTTLAFNGGTVRAGKTTAGFFGARPDLLDSRIQAGGLVLDTQGFAVT
                     LNDALESGATPDGGLIQLGGGTLTLAADCTYTGPTVVSNGTLTIQAAVASESLRLAPF
                     TTATVVSNDFFAGSLTVGGNARFLFTPGMTDSLSLTGMTLGDAQGAGQLVFEVTDEEN
                     TDRLDVTGPLDLSGKGDVFLYQPGTTTAFTANGTYALITFSGALTGSASDLRVANPDP
                     TKQYAFTESDGVISVTISERSGARWIADADGDWSVPGNWDGPVPNAAGAFAAFLTNAT
                     APVTVTLDAPVTVGNLVLSNNVVPYTFSGTNVLTFDSGSQPSFLSLQSGQHEMAVPWV
                     LADEMRVETAAGASLTLSDAVSGIGPMTVTGAGGVTLLGTNQTLTLLRNGFLSIADGA
                     ALAGGVTFDNGTLEITQSTVLDAPLEVGLRGAGVRPATNVTLTLASESQGGGKLRQNG
                     SGALSLAAPLGNIGGVSVESGTLLFTNDVFGSMPLELAGGTARYAGSENTTLGTPLVV
                     SASSQLAADSAGLTLDGPVAFPAVATLGLDVPNGLTLAGQATLSESGRKLHLLRGTLR
                     FAAGADYTLFSGARDTLKLGQDANAATELVIEEGARLRTGGIHLNTTLADATQCVALV
                     RQEGGIVEVTDGQGLFIRDYGYSDGIYQMNGGIFTAPVSSWTSVGSKGPGYLTVNGGT
                     MTLGRFAMGVNDFSSHVWNGPGGHLAINNGRLTIAGQCSWMSDAYRSRYNTVVLGNGT
                     PESGELNLVATTRAVPYASGQGGRTAFTFNGGVLKTIGVAPYEPSSLSDYLFGVDTLT
                     LRSGGAIIETPHADVALQQPLRAAEPMGGLIKRGTAALTLAHSNNVWCGETVVQAGTL
                     RARLNQRPQRLYPEGLLALWTFDDGTSNDQSGNGFHLLQQNDTNLVSFVEGGALGKAA
                     RFTGLSSLKMAYTSVFDITSFTVSAWFKLASKGGHQGIFSGRVNAAENSTGGTFDFKY
                     NDGDYISNLIPGNSGHGAHIKPEMGGRVAIGEWHMVTYVVVPGRVDAYLDGEWKAYTN
                     GLTSALLTPGFLLTLGRGIATSDNPNREMMRDGGMIDDVAVFSRALTAAEIATLCAEA
                     VTPRPAVRVGESAVYDLLGSTNDTPSVAGGGTISNGVLAVSERLEPEGEMETATLTVS
                     HLLLDSTNLIYACTVTDTTNDLVRVSETLEVRQAGSIDLGRTELDPLSTPWRRTVMTF
                     GAIDAADAARLSRWKVTGDGITGKNILRTVIVDTAQGTVDVELRFAGTFMLLR"
     gene            complement(20403..>23032)
                     /locus_tag="PLZ74_02085"
     CDS             complement(20403..>23032)
                     /locus_tag="PLZ74_02085"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97141.1"
                     /translation="FPRHRLERDWLYQDAGLDIAQVFTSPNDAAKERAMVQRVVGCVP
                     VDDPAAKTLRQRLESLDTGRVPARDRRWRDLYLDACAARRKVRLRDIRAKADRFIYVK
                     HGIFGCMQGLVGKYDIPDEQVRDFTPGFKRGGQLCMGTLLEDGAVKHEVLIEKPEGAI
                     CYPSLSWDAQTVVFAMRDHFDTDSYYLYTMDVASRKVRQITFPIVKDGKTLHVADCEP
                     VFLPDGRIAFTSTRDVHISDCWYRAGGNIYTCNIDGSGIRRLTSDQLMTSNPGVLEDG
                     RIVFTRWEYNDRSALYLHPLIAMNPDGTSQTEYYGNNSMFPAAIIHARGIPGSHKAIA
                     VIAGHHSIYKGKLGLIDRTEGTQAGQGIEFVNTRLNGGLGRESADFIRPEPPDPARPY
                     HDFKIDVFGQFGPQYAHPFAFDEENYFCSFCPEGYLPTFGPFNPPLGLYYMHADGARE
                     LLAYDDWQSTGRAVPVMRRTPPPVRPSHIMPTHNHGIYTVQDVYQGPGLQGIPRGTAK
                     KIRVVALEYRVCRMGYGQNGGECETGLCQTPISLNSGSWDVKHVLGEVDIEADGSCSF
                     EVPARAPVYFQVLDAHGYAIQSMRSWSTLQNGEHFACIGCHESKHDAGAHAASSAKTV
                     LALAKPPQKLKSFGGREHPLIKRLKTQSWRDSVENYLGVNAPRSLDADAPVDGFSYVQ
                     EIQPIFDKHCIRCHNADNIATCKISLTGEPAKPETIKLIGAGQVDPKRTYTHSYVAIT
                     TSGNPDNNPWMTWLKPRSRAVMLPPYHTGACKSKIMAHFEPGHHDVKVDDVEKRVFAC
                     WLDLLVPFCGSYTQHNTWTDAEKAEFHYFQEKRRAYAAKELIGWGVTHLQSQCPSALA
                     SATRQRVPQVRQQTVPSDH"
BASE COUNT         4373 a         7352 c         6771 g         4536 t
ORIGIN      
        1 gacgttcccg atcccgatcc cgatagcgat cccgaccccg atttggatga aaccctgaaa
       61 ccagaaaccc tgaaaccatg aaaccatgaa accatgaaac cctgaaacca tgaaacccga
      121 attgcggcgg ttcagcgccg tgtcacttgt tgaacccctt gtggttcatg aattcccaga
      181 tacggcccat ccaggtgtaa ctaccggtac ccggggaggc tttccgctgg aagcagtggt
      241 tgcgtgtggc cagcgtgtgg agatcggact ggatccccat ccggcgaagc tgctcccagc
      301 atttcaccga attcatcggc gcccagctgt ccgcgtcgcc gtggatgaac agaatcggac
      361 acgtcgcgag gtcgaacgag aattcgggaa cgggccgcga atcgtcctcg ttgccgccct
      421 tcgcgttcgg agcgttcgaa ccgtccgtaa gcgcgtacgc cggatagatc gcgaccgccc
      481 actgcacgtt acagggaatc ttatcgaggt cgtcgatcgg ccaatacgaa cgtcgtttcg
      541 aactggtcgc gcccatgagc gtcaagtgtc cgccggccga ggagcccatg ataccgatgc
      601 ggtcgggatc aagccccaac gccgcggcct gactgcggac gacccgcacg gcgcgttgca
      661 agtcctgcca tgcggtcgtg tgtttggcca atccgcccag cggacgcggc gtgcgatatt
      721 tcatcgtcac gaccgtcata cctttttcat tcaggaagcg ccgcgtcggc gcgacctcga
      781 aaccgtcagg actgttgcca ccgtagccgc cgcccgaata gatgatttgg atcgcctttg
      841 tcttacgctc cttcggcatg tgccattcga gataaggcaa accctgattc gtctgcacat
      901 ccggcatccg gccttccggc cagaccggtt ctttgcgata cccgtcgcgg gcatcgtcat
      961 tcggataacg ggtcatcagg tcgacttcct cgccgaggcg tccgtcgaaa ttcatctggc
     1021 gaaggtactc ggcgatccgg tcgagccaat tgtcgtaggc ggtgccttgt tcacccttgt
     1081 ctgggttacc catgaagccg tgcggccgat ccgcgaaaag gtgaatctcg gcggggattt
     1141 tcatcctgcg gagttggcgg taaatctgcg tggagccgtt cggcgaatag gcgtccatcc
     1201 cgccgtggaa gagcgccatc gagcaggtct tcgcatcgaa cttaaacacg tccgtgagtt
     1261 tgacatcgat ggcatcgcca tcgcgcgtgt tgggaccggt cagcccgtcc gtgagcgcgt
     1321 aggcggtgta gatcggcacg gcccagttga tgtggcacgg aagccggtcg aggtcgtcaa
     1381 ccggcccgta cgccggcgtg agtgcgcttg tggcgagcaa gaccgtcagg tgcgagcctg
     1441 cggaaaagcc gaacgcgccg atcttctccg gatcgaatcc gcgcttctgc gcctcgttac
     1501 gcacaaggcg gacagcgcgc tgtccgtcct gccacccgct ttgatagatc ggcagacctt
     1561 gcggacgcgg tgtccgatag gtgaggctga cacagacaaa cccgagatcg atcagcttct
     1621 tggcgacccg gtcaatccag accccgtcgc agcagctctg ataaccgccg ccggaaatca
     1681 acagcatgca cgcgccgttt ttttgtgccg gcgcctcata ccagtcgaga tacggcatca
     1741 cgcgctccgc cggtttgaat cccggctctt tgacctcctg cgtggtcgcc gcgatctgcc
     1801 gaggctggaa atccggaatc ttcccttccg gccacagcgc gactcgctct ccggcacacg
     1861 ccagaatgac gaccccgaac ccccacccca acgccagcat tgttttcatc atttcgtttc
     1921 cttttttcag aaggtcatgt cctggtacag gtaacaggtg atctccgcct tctttttgag
     1981 ctcggcaaag aaggtttcca cgcctgtgtt gtatttgcgg cgcagaatgg tgtcactcag
     2041 gcgcctccgg ttgacaggca ccgatttgat caaaatatgg gacgcgcgga cggtttcggg
     2101 caccgcgggc gtagtggagg tcgcgacttt agccggcata cgggaggtgg tcttgatgat
     2161 gtgataaccg aagcgcgtct cgatcaccgg cccgatctcg ccgatctttt gcgaaaaggc
     2221 ggcgtcctca aacggcttga ccttcttgcc gcgtgcgaat tcaccgagat ccccgccgtc
     2281 cttggcactc gggcactcgg aaacgttacg cgccatatcc gcgaaatccg ccccgtccaa
     2341 caactgtttg cggatcgtct ccaatcgcgc gcgccggagc tggttggttt cgtggagcac
     2401 ccctatcgct tccgtcaatt ccgcttcttc gacgcgaatc ttgtcccgca cctcacgttt
     2461 gagcagcttg tcgatgatca gaccgtcctc gaactcgcgc cgcatcatcc gggggggttg
     2521 cggtcccctc tcgaaaaaat cgttggtggt ccagttgcgg gacttgagcg tgttctgcaa
     2581 cgcacgcaga ccggcctgcc gatccgcctc ggtcaccgcg atcttccgct tcaaggcctc
     2641 atccagcatg acggttttga acacgaaggc gttgatcgcg cgacggcgga agtgttcgcg
     2701 ggcctcttcc agccggttgg acgggatgac cagatggttg acctccacgt catccttgag
     2761 atagcccatg gcgcggcggt ccatctcctc ccacgtcagc ggcgtcccgt tgactttcgc
     2821 gaccacatag tccggcgacg tgacaccggc ggtcggcgcg gaattcgcgg cggactccac
     2881 ttcagcggcg ggttcggatt tcttgccgca tccgctccag caacacacca gcaccagcaa
     2941 caggctgaca ttcagacgga tcaaaggtcg catacggttt ctcctcgctc ggggggttca
     3001 gaaggtctta tggaaggtac ggtgaatcac atccaactgc tgttcgcgcg tgagtttaat
     3061 gaagttcacc gcatatccgg agacgcggat cgtgagttgc ggatagttct ccggatgctc
     3121 catggcatcc agcagcatct cgcgctccag cacgttcaca ttgatgtggt ggcccttctc
     3181 gctgaagtaa ccgtccagca ggccgaccag gttttcgacc cgctcgccgg gtgtgcggcc
     3241 cagcgcgttc ggcacgatcg agaaggtgaa ggaaatcccg tcctcgctgt ggtcgtaggg
     3301 cagtttcgcc accgatttca tggaagcgat gcacccgcgt gaatcgcggc cgtgcatggg
     3361 attcgcgccg ggcgcgaacg gttcgccctt tttgcgccca tccggcgtcg aaccggttgt
     3421 tttgccgtac actacgttgg aggtgatggt gaggatcgaa agcgtgggct tggcgtggcg
     3481 gtaggtgcga tgtttcgaaa gccgccccat gaaggtcttg atcacttcga cggcgatctc
     3541 gtccacccgg ggatcgttgt ttccgaaggt gggatattcc ccctcgatct cgaaatcgac
     3601 ggccaaaccg cgcgcgtccc ggatcggacg gaccttggcg tgcttgatcg ccgaaaaact
     3661 gtccgccacc accgacaacc cggcaatgcc gcaggctgcc gtacggaaga catcctcatc
     3721 gtgcaacgcc atctgcaacc gctcataaca gtacttgtca tgcatactgt ggatgacatt
     3781 gagggtattg atgtagagcc gggagagcca acggcagatc tggtcgaatt tccgcacggt
     3841 ttcctcataa tcgagcacgt cggaggtgac cggggccatc tcggggccga cctgctgccc
     3901 cgttatctca tccttgccgc cgttgatgca gtacagcagg gctttcgcca gattggcccg
     3961 cgcaccgaaa aactgcatct gcttgccgat ccgcattgcg gaaacgcaac aggcgatccc
     4021 gtaatcatcc ccgaacttct cccgcatcag ttcgtcattc tcgtactgga tggacgaggt
     4081 cgcaatggag agccgggcgc agaaccgctt gaagggctcg ggcaggcgcg ggctccacag
     4141 caccgtcatg ttcggttcgg gggccggccc gagattgacc agcgtgtgca tgaaccggta
     4201 ggtcatcttg gtgaccatgt gccggccgtc cacgccgatc ccggcgagcg attccgtgat
     4261 ccaggtgggg tcacccgaga aaagcgcgtc ataattgggc gtccgcagaa accgcaccat
     4321 ccggagtttc atcacaaagt ggtcgacaaa ctcctggatt tcggcctcgg tgtaccgccc
     4381 ggcagccaga tcgcgttccg catagatgtc cagaaaggtc gagatgcggc cgatcgacat
     4441 ggccgcgccg ttctgctctt tgactgcggc gagataggcg aaatagagcc attggatcgc
     4501 ctcgcgggta tcccgcgcgg gctgcgcgat gtcatagccg taggaggcgg ccattgcctt
     4561 gagttcctgc aacgcccgga tctgttccga gatttcctcc cgcaactgga tcgcctccat
     4621 gtccatggcg tcaaagacat ggcgcgcctt ggccgcctct ttatgcgcga tcaggaaatc
     4681 cagtccgtac agcggcacgc gccggtaatc gccgatgatg cgcccccggc cgtaggcgtc
     4741 gggcagaccg gtgatgatgc cgttgtgacg cgcgagcttc atctcatccg agtacgcgtc
     4801 aaacaccccg tcattatggg tcttgcggaa cctgaagaca tcttggatcg cctgcggcag
     4861 ctcccggccg tacgccttga gggaggactc gaccatacgg atgccgccaa agggcataat
     4921 ggcccgctga agcggcgtgt cggtctggat accgacgatc tgttcgagtt cgcggtcgat
     4981 gtatccgggc gcgtgcgagg tgatggtcga gatgcggctg ttgtcaatgt cgtaaatgcc
     5041 gtttcgggcg cgttcctcct ccatcaggcc tcgcacctga tcccaaagtt tccgggtccg
     5101 cccggtcacc ggggcgagaa acgcgtcatc cccgtcgtac ggggtataat gtgaaatgat
     5161 gtgatcccgg acattgatgt taatccgcca gatatctgaa gagagcgaca tgcaaccccc
     5221 cctgtccgat cccgcgccac cgcgacgctt catgttgaaa ctggtgcgcc tggcgggggt
     5281 cgaacccaca accttcggct tcggaggccg acactctatc cggttgagct acaggcgcaa
     5341 aaagtggggg ttatgataat gactgtcagg tcggacttca agcccgttgt tcatcccgga
     5401 accgaaaatc cgaggcacac ggcacagtaa gccccctgtt cagcccgcgt cgtcgtcttc
     5461 cgcaggagcg gtgatcttct ccgtttcccc atcctcctca tcatcaagag gacgggtcgg
     5521 ttggaaatcc tgacaggaga gattccagga gagccatgcg tcgaacaggt tggcgttgcg
     5581 gcggcgcgcg atggcgttcc ggatttgcgc gcgcatcatc tcggccgcca gtgcgtcacc
     5641 cggctggcgg tcctgcacat aaaccagaag cgcgcgggcc gcggagaccg gaatgatctc
     5701 ggaaagctcg cccttgcgca gcgtcatggc tccgtaggcg atcgagaaac tgttctcaaa
     5761 cttctggttt tggatgtcac tgaccgtata cgtgagggcc gtggtgacgt tgagcgcttt
     5821 ttcccgggca gcttcggcga acgatttgcc ttcgccgagg cgcttcaaca gatcctcccg
     5881 caacgtcttg gcaacattgt cgaacttctc ggtacgcgcc ttggcttggg ctctcggctt
     5941 caactcttcc aagaccgcct catacgtcgg cgtatgcgct tccgaacgtt ccagcggctc
     6001 gatcacgtaa atgaactgct gccccttgac gataccgaag cgtgaatccg cgcgctcggg
     6061 atccaattcg aaggcggcat ccgcaaatgc cttgctgtcc tcgacccaga acaacgcctc
     6121 ctcagcgccg aagagcggag aacgtttgac gggcaggcgt tgctgcattg aaatctgatc
     6181 gagcgcattg gatcccgccg cggtcagctt gccgtacacg gtgaaggtga cactcgtctc
     6241 cgcgcagtac cgggcctcct ccagcatcag ttcggcgaga atgttcgtgc gcacctcggc
     6301 aaaggggatc ggttccgtcg tgttgttggt ggtggtccgg gtataggtgt tcgcgtggct
     6361 gtcgtaatat tccagcaggt catcctcggg cacggaaacg gccgcgagat aattggagac
     6421 cggaatcatg acataccgca cggacatccg gtccggcagg gcgaacgagt ccttgttctc
     6481 ctcataatac tttcggtaat cggcatcggt cagcggcatt gcggcttccg cgaagcggtt
     6541 gctgactgcg gcgacctgca ccgtaaagat gtcggtcatg gcggcgagct catcctccag
     6601 ctccatgggc gcaacccagg cggcggtttc gacgagcgcg gcgtttttca tgattgccaa
     6661 ctggtggctc accatgttct catacagggt gggcgtcaag ccctgctcgg cgagcaccgc
     6721 gcggtagcgg ttgagatcaa acccgttggg gccctgaaaa cccggcgcct cacgcaagac
     6781 cgcgcggatc tcgtcccggc tggccgccaa accatttttc cgcgccacct gccgcgccgc
     6841 caactgttcc catgcacggc ggtccaccac gctggcgggg gtcgtgttgt cacggttctg
     6901 gccgaatccg cggatcgtct gcacaacccg atcgaactcc tcagaggtga ccgttttccc
     6961 gttcagtttc ccggcggcca tccgatcctg aggccgctgg aaacaggaat ccacggccac
     7021 aaacgcaacc gcaatgacaa taacgaagaa gccccagaga atccggctac ggatgaggcg
     7081 gttgaaatga taaatgaaca cgattttttt ccaaacgggg ttgagtacgc gcaacgttta
     7141 tcacaagaaa aaccggtggc tgcgcaggcc gccggtcatc ccccacaaca cgccccttcg
     7201 atcagggctc cttctgcccg tccgccgcag gcgtggcggg cacggccggg gctgcggagt
     7261 cctgcgccac accgtccaaa agaactggca acgcctcttc tttcggttcc ggttccgcgg
     7321 ggacaatcaa ttcgccggtg gtcaggtccg gacgcccctc cgcgtaaacg aaacggtgcc
     7381 gggcgattcc ggtggggctc acaaccaagg tcgagatgat cgggcggcca ccgaccggcg
     7441 cgttctcacg ccagatcttc accacggctt tgggagtcat gccgaaggtg accggtgtct
     7501 cggtgccgtt ttccagcacg atcgggaaat cgcccgacac gctggtgagc cgggtgagcg
     7561 tgcggtcagg cgcgttctga atgttcaccg tattggcggc gcgcagtgcc ggaatgtggt
     7621 attgcgcgcc ctccacaagt gccgaaccgg tgatcgtggc ggcctgaaag gtttccagcg
     7681 taccatcaca aaccagcgtg tactctcccc gacccgccac attgcggcag gtggtgctgg
     7741 gggtctcgat cgcgaacagc ccctcctgaa gattatcccg gagcgtcgtc ttcatggcgc
     7801 cgccgctgaa acgcagcaca cgggcctcgg gattcttttc cggcgctgtc accacaacct
     7861 ccgtgttcgc ggtgagcttg acgctctcct gagcggagaa caccagcagg gccgacccgc
     7921 ccatgccaat gcggaacacg gttcccaacg gatagtattt gttgttcgtc gccggcgcga
     7981 acccctccgt ccccggtatg ttcacctcgc acacgcccaa cacattcatc aggcgcacga
     8041 gcggatcaaa caccttgacg gcggccgcgt cactttcctg tgccgccgca ggcaccgcct
     8101 cacccgcaac aggtgccgca ttttcctgag ccagggccgt cagcagagct aaccccatca
     8161 cgcaacatcc cacccgcaac aatcctgtct gatccatcat ggggacgact ccctcgttcg
     8221 catgccgttt caacaaacct tctacctcaa ataaaaaaga tgaggaattt tagatcaaac
     8281 ccgtcagata gtcaatccgt gcttcaccgg caaacgtttt tttcacaccc tccgtttctt
     8341 cgtttccgac ggcgggcatt ttcgtgttcc gtattgggac cgaatccgtt agaatctgcc
     8401 tcgttcccaa acctacatgc gctgaaaaag gatgtaaatg tgaaaatctc gaccaaggga
     8461 cggtacggac tccgtatcct gatggacatt gccctgcatc aggacgcggg cgcggtgacg
     8521 ctgaatgaga tcgcgcgacg ccaaggaatc tccgtgaaat atttatggca ggtgatcaat
     8581 ccgctcaaga ccgcagggct cctgagtgtg acgcgcggtg cgaaaggcgg gtacatgctg
     8641 gcgcggtcgc ccgaacaaat cacggttctg gacatcgtgc ggattctaga gggctccgtg
     8701 gtgctgctgg actgcctcac gaataagaag cattgcgacc agatggacat ctgcgtggcg
     8761 cgcacggtct ggatggaggc gcagaacgcc atggaggacg tgctgcgagg cataacgctg
     8821 gaaaccgtac tccgccgttg cgcggacgcg accgccacag ccaacgacta ctcgatctga
     8881 cgcaccgcgt cgggccgcgg catacgaacg gatcccgcct cgctcatttt tccggcgcgg
     8941 aaaccgcagc gggcgccgac tgtgtcacgg caccgggcac ggctaacgca ggcggcacgc
     9001 tttgcgcggc ctgatccgcg gcatcgaaca gcgaacgcgc ggcgcgcggc tcataccggt
     9061 cgacaacccc ctgaagctgg gcgaccgcca tctcccaggt cgcgtcaccc gtttttgcgg
     9121 ccttgaggtt ttccagccgt ttccgggctt cgtccttttt accctgcaac gtcaacacac
     9181 gcgcgatccc catcacggac tgcggctgca gaaaatgatc cgtccggtca cgctcgaatt
     9241 cctgataggc ggtcagcgcg tcatccagac ggttcagcgc ttccagcgca tgcgcgcggc
     9301 ccaactccgc gatctcccgg aacccttgcg gcgcgccttt tttcaggcag agttcgtaca
     9361 ggtcgagtga ctcctgtgcc ttcccggcat cgaaatacgc tttggccaga cgcagccgcg
     9421 cggcgtttcc ggccttgaac gagccgtact gcgccaccac accctcgagg tcctccaacg
     9481 aatgagcctg gaggaacgcc tgattggcgg ccgcgatttt cgccgtacgg tagtacttga
     9541 acgcccagac cgcaccgcaa accagcacaa cggcaccgaa ggtgatcagc gattgcggac
     9601 ccttcgaccg ccaccagtca tacaagggca gcaactcttc cggcaggttc tccggaatgg
     9661 ttttcaaatc cgttttggcc gactcgtccg cacgggcatg cttttgactc atgttttccg
     9721 cctctgaaaa gggtaaaaag ttttagtatt ggggttccgc agacgaaaag caagaccaaa
     9781 gaggacgaaa tcacgttcgg aacgggaatg cgtccgtgtt ccgcttgttc ccctgccgta
     9841 tttcctgcta tgatctgttt ttatggatgc cggggtaaac cggtgttccg cccaacaatc
     9901 ggtatggagg acccgtgaag cagacacatg acaccgttgt gattcttgat ttcggcgcca
     9961 tcaacagcca ggctgcggcc aaactcatcc ggcagctcaa catctattgt gaagtgctcc
    10021 cctgcaccgc cgcgcgtgaa cggatcgaag ccctccgccc caaagcgctg gtgctgcagc
    10081 gcggcgcaga gttcggcgcg aacaccctca aacgctcgcc cgcgacggat cccgctgcat
    10141 ggggagtccc ggttctggat ctgtcggatc tgccctgcga gatcgcgacc ttcgaaacct
    10201 ttctcctcag ccagtgcggc ttgcgtcagg attggacggt cgaagcgttc atccgcgagg
    10261 cgatcgacga aatccgcggg atggtgggat cgcaccgggt tctgctggcg atgtcggggg
    10321 gagtcgactc ttcggtctgc gccctgctcg cgcaccgtgc ggtcggcgac cggctcactt
    10381 gtgtgttcgt ggatcacggc ctgatgcgca aaggtgaatc ggatgaggtc tgttctgtgt
    10441 tccgcgaccg tttcgggatc ccgttgcgcc atgtcgatgc ggcggagcgt ttccttgcgc
    10501 ggctggcggg catcaccgac ccggaacaca aacgcaagat catcggcgag gagttcatcc
    10561 gcgtgtttga ggaagaggcc gccgcggcgg gcgagccgga tttcctggtg caggggacca
    10621 tctatcccga catcatcgaa agcggatggg acggcggaaa accggtgaaa gcccaccaca
    10681 atgtcggcgg cctgcctgac cgcatcgcat tcaaggggtt ggtggagccc ttgaaatatc
    10741 tgttcaaaga tgaggtgcgt caggtggcac tggccttggg aatcccggcc gcgatcgcca
    10801 tgcgccagcc gtttccgggc cctggtctgg gtgtgcgctg cctcggcgaa gtcacccggg
    10861 agaaactcga tattttgcgc gaagcggact tcctgttccg gaacgcgatt gtggcggcgg
    10921 atctggaaac gcaggccaac cagtattttg ccgtgttgac cgacagccgt tcggtgggtg
    10981 tcagccagga cgcccgaacc tatgcctaca ccgtcgcgct tcgggcagtg cagacatcgg
    11041 acttcatgac cgcgcaattt gtgaaaatcc cgctggatgt gctggaatcc gtctccgaac
    11101 agatcacccg cgaggtgccg caagtgaacc gcgtcgtgta cgacatcacg aacaaaccgc
    11161 cgtccacgat tgaatgggag tgacggcata cgaggccgcg ccatacgcgc ctgcaccgga
    11221 aaacaatgag gggcacagct tgtaacaggg agcaaaccat gcaaaacttc gaaaaactcg
    11281 gctcgttcta tctaggtaag gaatatgatc tcgcggcagg ggcgcggaca gacaacctcc
    11341 tcttgtatga cgcaaaagac ctgacgaccc acgccatgat cgtgggcatg accggaagcg
    11401 gcaaaacggg cctcgccatc tcgctgctcg aagaggcggc gatcgacggg attcccgcga
    11461 tcctgatcga tccaaaggga gatctcgcga atctgatgtt gcagttcccg gacctcacgc
    11521 ctgatgcctt tcagccctgg gtcgatccct ccgaagccac acgcaaagga gtgagcctcg
    11581 cagatctcgc ggcggccacg gctcaaacct ggcgcaacgg actcgccgag tggggtcagg
    11641 acccctcccg catccgccga ttgcgggacg gcgcggagtt cgttatttac actcccggtc
    11701 acgcttccgt gcgtccgctc cagatcctgc gttcgttcgc cgcgcccccc ccggcactcc
    11761 ggagcgacga tgcggcgttg cacgaccggg ttttgacggc cgtgtccggt ctgctcggct
    11821 tgctcggcct cgatgcggat cctgttcaga gccgcgacgc tatcctgctc tccaatctgt
    11881 tttgtcacgc ttggcgggaa ggccgtgatc tggatcttgc cgccctgatc cgtgacgtgc
    11941 agaaaccgcc cttcgcccgt gtcggcgtgt tggacctcga aaccttctat ccctccgccg
    12001 aacgcatggg gttggttatg cgcctcaaca atgttctggc atcgcccggc ttcagcgcct
    12061 ggatggaggg tgaacccctt gacgtgcaac gcctgttgtt ttccccgacc ggcaaaccgc
    12121 gccatgcgat cctttatctg gcgcatctct ccgataccga acggatgttc ttcgtgaccc
    12181 tgttactcaa cgaggtcacc gcatggatgc gggcccagtc cggcaccggc agcctccggg
    12241 ccctgctcta catggatgag atcttcgggt actttccgcc caccgccaac ccgccctcga
    12301 aaacaccgat gctgaccctg ctcaaacagg cccgggccta cggactcggc gtcgtgctcg
    12361 ccacccagaa ccccgtagat ctggactaca aaggcctttc gaactgcggc acctggttca
    12421 ttggaaggct tcagaccgaa cgcgacaaga aacgggtgat cgaaggcttg gagggcgcgg
    12481 ccgcaagcag cggcgccggc ttcgaccgcg cacggaccga acagcttctg gccggtctcg
    12541 gcaaccgcct gttcctgatg cgcaatgttc acgaggagga accggtgctg ttccaaaccc
    12601 ggtgggcact gagttacctg cgcggtccgc tgaccctgcc gcaactcgcg accctcgcgc
    12661 cgccgcggga cacgatcccc gaggcgcaac ccgtaacgca gacggcggcg ctttccggcg
    12721 cgtcttttcc gactgccgcc acacccctac ccccagccgc agcccatccc ctacccccgg
    12781 acctgcccgt cttccacctt cgccccggcg cgtcattttc cgccgacctg gtttaccgac
    12841 cgatggcggc aggccttgtc aacatgcgct ttgtcgatgc gaaagcgggg ctcgacttct
    12901 ggatgaacgg gatcgcgctc gcgccgtttc tggatgaggg tgcccgcgcc gcctgggagg
    12961 agtgcgaaat ccggcgcggc acctcctctg cccttctgga tcctcaaccc ttgccgacgg
    13021 tggcgttcga aacaccgccc gttcacctgc cggggtccaa acaactcgcg tcctgggcca
    13081 aggatctgga aagtcatctc taccagaccc acacgttcga gctgctcacc tacccggatc
    13141 tgaagctcgc cgcgatgccg gatgagtcgg aaggggactt ccgtgcgcgc atcatcctgc
    13201 ggttgcgtga acagcgtgat gccgaactcg caaagtggaa agccgcgtac gcgccgaagt
    13261 tccagaccct cacggaccag atccggcgtg cggaagagcg cgtggcgcgc gaaaaagaac
    13321 aggcgggcca gcagaaattc aataccgcgc ttgcgttcgg cgccacactg ctgggcgcgt
    13381 tcctcggccg cgggggcgcc ccggtcggaa cagtcggacg tgccacgacc gccatgcgga
    13441 gcgcgggacg gatccgcaaa gagagcgggg acgtcgcgcg cgcctccgaa aatctcgaaa
    13501 cccttcggca acggttgagc gacatgcagg aacagttcga ccgggaactc tccgccctgc
    13561 aggccccggt cgcggccgaa gcgctgtcgc tcgtcaagcg cccggtgcgg ccgcggaagt
    13621 ccgatctctc cgtcggcacc gtgggactct gctgggtgcc gtggcgcaaa cggcccgacg
    13681 gcatgttcga gcctgcggga tgaccatgac aaaacagcgt atatccccaa tgtccgccac
    13741 ccccctgccc cctttaaccc caggttaact tatgtcctgc tcaatcccgg acacgcgaac
    13801 ccgactgctc ctcctgcgcg gtgtgctgcg ccgctttttt ctaaacctct tccgccccgc
    13861 ctatgtgcgc gacagccttg cgcgccgaca aggggcgtgc aaccgttgcg gtgcatgctg
    13921 ccacctcgtg gccaaccgtt gcgcacggtt gcgcgtttac ccggacggcc actccgaatg
    13981 ttggcggtac gaagggttcc gcatgccgaa ctgcaaaacg ttcccgatcg acgcgcgcga
    14041 tctggccgag cgcgactgga tcgcgcccga cacgccttgc ggttatttct ggccggatgc
    14101 gcccgtcact ccgcagcctt cccgcagatc atgatcatcg gcttgtctgc gttacatcaa
    14161 gtcctgcatg gtcacgaccg gcacatcctt cttgggcgcc gacttcagcg gacgcagccg
    14221 tttggcgttg aaacaccacc ccttctccgc gtccagcggc caaaccgcag gcgccaacgc
    14281 catcaccgtg gcggcaaacc cctgaagcgt ggccgcggca ttgcctgtgt gcgacgaatt
    14341 gtcggcctcg ttctcgatca cgaacggccg gtcaaacccg agctccatca gggtcttcaa
    14401 aaacgcgaac cagtcgatgc tgtcggaacc gccgaagccc ggcagtttcg gatcgtagtg
    14461 gtgccggtcc cactcggacg cgggctgaca cacccctgcg cgtttggcca acttcgcgtc
    14521 cacctgctgc atcggataga ggcgtcccca ttggatcgcc gcgagactgt tcgtgcggtt
    14581 acgggtcccc ttgatgtgaa cgcggcgtag ccggttcaga tccgtgttgc ggaccacatc
    14641 aaccggatcg atatgctgcc atgcgtcatg cgaggggtca tacgtctccc cgtgcgccgg
    14701 actcggaatg agcgcgtaca tcagcttgcg cgccgctaac gtgcagggca ggttgttgta
    14761 cgtggtggga gtcgaagccg ggcgccatcc ctccatcgga cagttctcat acgtgagcgt
    14821 cacccccaag tcttccgcat agcggacgat cggccgaaaa acgcgggcgt agcattcgag
    14881 gttcttcgca aacccgccat cctccttgcc gagttcatgg ttatagccca cgaacgttcc
    14941 gacgcacacg tcattggcgt caccgcccaa cagatgcgcg atgcggatca gcttgaggat
    15001 gtggttacgg ttggcttcgc acaccgccgg atcgccgccg agcagattgt cgtacgcgcc
    15061 gaccgaaaaa cgcaggccct ctttgttcgc tgtttccagc acccgccggg cgtcctccag
    15121 cgtaaaggtc tcgtaattca ggtgcgtggc cacatgatcc tgccgcgacc cgtcgcgccg
    15181 gaacacgcac aactcaagcc cctgagcacc cacagccttg gcgcgcttca ccacctgcgc
    15241 aaaggtgagt ttcggaaaag ccgaggtcat tagccaaatt ggattgttca tccctgtatc
    15301 cccttattcg attttgccac gcggccacgc cgcatgacgt tgtgatgcgc ggaccaacgc
    15361 aaaccgcact ggcccgcgcg tgaaatcgga tcgcatccct gccgcgctca acggagcaac
    15421 atgaaggtgc ccgcaaaacg cagctcgacg tcgaccgtac cttgcgcggt gtccacaatc
    15481 accgtgcgga gaatgttctt gccggtgatt ccgtcacccg ttactttcca ccgcgagagg
    15541 cgcgccgcat ccgccgcgtc gatcgcgccg aaggtcatca cggtacggcg ccaaggcgtg
    15601 gacaacggat ccagttccgt gcgtcccaga tcgatgctgc cggcctgccg cacctccagc
    15661 gtttcggaaa cccgtaccag atcgttggtc gtatcggtaa ccgtgcaggc atagattagg
    15721 ttcgtgctgt cgaggaggag atggctcacc gtgagcgtcg ctgtttccat ctccccttca
    15781 ggctccagcc gctcgctcac tgccagcacc ccgttcgata tcgtgccccc acccgccacg
    15841 gacggcgtgt cgttggtcga gcccaacaga tcgtacacgg cggactcgcc tacccggacc
    15901 gccggacgcg gcgtgacggc ctccgcgcac agcgtcgcga tctccgcggc cgtcagagcc
    15961 ctgctgaaga ccgccacatc atcgatcatc ccgccatccc gcatcatctc ccggttcgga
    16021 ttatcggacg tggcgatccc gcgtcccaag gtcaacaaaa atccgggcgt cagaagggcg
    16081 gaggtgaggc cgttggtgta cgccttccac tccccgtcca gataagcgtc cacacgtccg
    16141 ggaaccacca cataggtgac catatgccac tcgcctatcg cgacgcggcc ccccatctcc
    16201 ggcttgatgt gcgcaccatg cccgctgttc ccgggaatca ggttcgagat atagtcgccg
    16261 tcattgtatt tgaagtcgaa cgtcccccct gtactgtttt cagcggcatt cacccgtccg
    16321 ctgaagattc cctgatgccc ccctttggat gccagtttga accatgcgga aacggtaaag
    16381 gaggtgatgt cgaaaaccga ggtgtacgcc attttcagag aactcaagcc ggtgaaccgg
    16441 gccgctttgc caagcgctcc gccctcgacg aaactcacca gattggtgtc gttctgctga
    16501 agcaggtgga acccgttacc ggattggtcg ttagaggtcc cgtcgtcaaa cgtccagagc
    16561 gccagcaacc cttcaggata caggcgttgc ggccgttgat ttagccgcgc ccgcaaggtt
    16621 cccgcctgaa ccaccgtctc gccgcaccag acgttattgc tgtgcgcgag tgtcaacgcc
    16681 gctgtaccgc gcttgataag tccacccatc ggttcggcgg cgcgtaacgg ctgttgcagc
    16741 gcgacatccg catgcggcgt ctcaatgatc gcgccgccgg aacggagcgt cagcgtgtcc
    16801 acaccgaaca ggtaatcaga gagcgaagag ggttcgtatg gcgcgactcc gatcgtcttg
    16861 aggaccccgc cgttaaaggt gaaagccgtg cgtcctcctt gccccgaggc gtacggcaca
    16921 gcccgtgtgg tggccaccag attcaactcc ccggattcgg gtgtgccgtt gcccagcacc
    16981 accgtattgt agcgggatcg gtaggcatcg gacatccacg agcactggcc cgcaattgtc
    17041 agacgtccgt tgttgatggc cagatgccct cccggcccat tccaaacgtg gctcgaaaag
    17101 tcgttgaccc ccatcgcgaa acggccgagc gtcatcgttc cgccattgac cgtcagatac
    17161 cccggaccct tggatccgac actcgtccat gaggagaccg gagcggtgaa gattcccccg
    17221 ttcatctggt atatgccgtc cgaataaccg taatcacgaa taaacaaccc ttggccgtcc
    17281 gtgacctcga caatcccgcc ctcctgacgc accagcgcaa cacactgggt cgcgtctgca
    17341 agagtggtgt tcagatgaat cccgcctgtc cgcaggcgcg cgccctcttc gatcaccaac
    17401 tcggttgccg cgttcgcgtc ctgtcccagt ttaagcgtgt cgcgcgcgcc gctgaacagc
    17461 gtgtagtctg cccctgcggc aaaccgcaat gtgccgcgca acaagtgcag ttttcgtccc
    17521 gactcactca atgtggcctg ccccgccaac gtcagcccgt tgggaacgtc gaggccaagc
    17581 gtggccaccg cagggaacgc gaccgggccg tcgagcgtca gtccggccga gtccgcagca
    17641 agttgggagg aggcgctgac gaccaagggg gttcctaacg tcgtgttttc actcccggca
    17701 tagcgcgccg ttccacccgc gagttccaaa ggcatactgc cgaacacatc gttggtgaac
    17761 aggagcgtac ccgactcgac cgacaccccg ccgatattgc cgagcggtgc cgccaagctt
    17821 agcgcgcccg acccgttctg cctgagcttg ccgcccccct gcgactcgga cgcgagggtc
    17881 agcgtcacat tcgttgcggg ccgcaccccg gctccacgca atcccacctc cagcggggcg
    17941 tccaaaaccg tgctctgcgt gatctccagt gtgccgttat caaacgtgac tccacccgcc
    18001 aacgcggcac cgtccgcgat ggacaggaaa ccgttccgga gcagggtcaa agtctgattc
    18061 gtccccagaa gcgtgacccc gcccgcgccg gtcaccgtca tcggcccgat gccggacacc
    18121 gcatctgaaa gggtcagcga cgcgcctgca gccgtttcca cacgcatttc atctgccagc
    18181 acccagggca cagccatctc atgctggccg gattggagcg aaaggaacga gggttgggaa
    18241 ccggaatcaa aggtcagcac gttcgtcccg ctgaacgtgt acgggacgac gttattgctg
    18301 aggaccagat tgccgaccgt gaccggcgca tcgagcgtca ccgtcaccgg agcggtcgca
    18361 ttcgtcagaa aggccgcaaa cgctccggcg gcatttggaa ccggaccgtc ccagtttccg
    18421 ggaacggacc agtcgccgtc cgcatcggcg atccagcgtg caccgctgcg ctctgaaatc
    18481 gtcacggaga tcaccccgtc actctccgtg aacgcatact gtttcgtggg atcgggattg
    18541 gccacgcgca gatcggatgc cgaaccggtc agtgcgccgc tgaaggtgat caacgcgtac
    18601 gtgccgttgg cggtgaaggc cgtggtcgtg ccgggctgat acaggaacac gtcccccttg
    18661 ccggaaagat cgagcggccc ggtcacatcc agccgatccg tgttctcctc gtccgtcacc
    18721 tcaaagacga gttgacccgc gccctgagcg tctccgagcg tcattcccgt aagcgagagg
    18781 ctgtccgtca tcccaggggt gaagagaaag cgcgcgttcc ctcccactgt caggcttccg
    18841 gcaaagaagt cattcgaaac gacggtcgcc gtggtgaacg gcgcgagccg gagcgattcc
    18901 gaggccacgg ccgcctggat cgtcagggtg ccgttcgaaa cgactgtcgg gccggtatag
    18961 gtgcagtccg cggccagcgt cagggttccg ccgcccagct ggatcagtcc gccgtccggc
    19021 gtcgcgccgc tctccagcgc gtcattgagg gtcaccgcaa acccctgcgt gtccaaaacc
    19081 agccccccgg cctgaatacg cgagtcgagc aaatccggcc gggcgccgaa aaacccggcg
    19141 gtcgttttcc cggcgcgcac ggtgccgccg ttaaaggcga gtgtggtgag cagattggtt
    19201 ttcgcgctgt gcttaaaccc gccggtcagg aaaagtccgg cgttgaagtc gatccggttg
    19261 gtgatcgtcc catctctctt cggattcgag agcctgtacc ccacatcgaa ctccccggcc
    19321 ccgtcggccg agaatttgaa catcccccca ttcaggataa cgtggcttcc gccgttctcc
    19381 gccaattcct gtccggtcgc aagccactcg aacaagccgt caccgcttag cgtgagcaac
    19441 gtgttaccgt tgtttgacca gcgcggcgcc cctcccgcag tgaacacgcc gccccggaca
    19501 ttgaccacgc aggtcttcgt ccggtcctgc gccgtgagca gcgagttccc cacgacgatc
    19561 cggccgctgt agagatccac ggtgcatgct tgccagaaca cataggacgc cgtgaaaccg
    19621 gaaagcgtcg cgttgttcgt gatgatgagc gacgcattgt tctgcagatc caaccgattg
    19681 aacaccgcat cgctggccac aatcaggctt ccgccgttga tcttgctgac cgggtcgacc
    19741 gccgtgaaat tcgcgccgcc ctcgaggatc agccgtccgg atccattttt cgtgaacgcg
    19801 gcgttcccgg caccgctggt cagatgtccg gcgagaacca catcccccgc accggtgtcg
    19861 agcgtgcggc tcaccccgga ccaggccaca tccgccgcaa tacgctgcct ccccgttgag
    19921 ttgttggcga gattgccgca cagaatgaac gggttgccgg agaggttgaa cggcgcagag
    19981 gacgccgcaa aagtcaggtt ggaaaagaaa gaccccggct ggaaatcgtt gaccgtctgg
    20041 gtcacgcttc cgtcgaacgt gaggctggtc agcggaccgg ggaccggtgc cgccccaccc
    20101 tgccaattgc cgcctatgga ccactgcggc ccggcaccgc ctccggtcca cacactttgt
    20161 gagaatccgc gacacgtcat cagaaccaga atcggcacca gaagaaccca caacctgctt
    20221 ctcattgcga tccctttctc ctttatttat ccgccatatt caagtgagtg tcacaagacg
    20281 gcaacatgat atggtgggag gataccgttt ccgccgactc ccgtcaatcc caagcaaaat
    20341 cggaaaacgt tttggggagt ttccgatgtt tgatcgccaa tcgctgtgcg cgggaagcgg
    20401 cctcagtggt ctgacggaac cgtttgctgc cggacctgcg gaaccctttg gcgagttgca
    20461 gacgccaacg ccgacgggca ctgactctgg agatgggtta cgccccatcc gattaattcc
    20521 tttgcggcgt acgcccggcg tttttcttga aaatagtgga actcggcttt ttccgcatct
    20581 gtccaggtat tgtgctgcgt gtatgaaccg cagaaaggca caaggaggtc aagccagcag
    20641 gcgaagacgc gtttttcgac gtcatcgacc ttcacgtcgt gatgccccgg ctcaaagtgc
    20701 gccatgatct tgctcttgca ggcaccggta tgatacggcg gcagcatcac ggcgcggctg
    20761 cgcggcttca accacgtcat ccatgggttg ttgtcggggt tgcccgatgt ggtgatggcg
    20821 acataggagt gggtgtacgt ccttttcgga tctacctggc cagcgccgat cagcttgatg
    20881 gtttctggct tcgccggctc gccggtgaga ctgatcttgc acgttgcgat gttgtcggca
    20941 ttgtggcaac ggatacagtg cttgtcgaaa ataggctgaa tctcctgcac gtagctgaat
    21001 ccgtccacgg gagcgtctgc gtcgagtgag cgcggcgcgt tgacccccag ataattctcc
    21061 acgctgtcgc gccacgactg tgttttcagc cgtttgatga gcgggtgctc ccgaccgcca
    21121 aaagacttca gtttttgcgg aggcttggca agggccagca ccgttttcgc agaactcgcc
    21181 gcatgcgccc ccgcatcatg tttgctttca tgacaaccga tgcaggcgaa atgctcgccg
    21241 ttttgaagcg tggaccaact tctcatggac tggatggcgt agccgtgcgc gtcgagaacc
    21301 tgaaaataga ccggggcgcg cgcggggacc tcgaagctgc aactgccgtc agcctcgata
    21361 tcgacctcgc cgaggacatg cttgacgtcc caactgccgc tgttcagcga gatcggcgtt
    21421 tggcaaagtc cggtttcgca ctcacccccg ttctgtccgt accccatgcg gcagacgcgg
    21481 tattcgaggg cgacaacgcg aatttttttc gccgtgccgc gcggtatgcc ttgcaggccc
    21541 ggcccctggt agacatcctg cacggtatag atgccgtgat tgtgcgtcgg catgatatgg
    21601 gacgggcgca cggggggcgg tgtccggcgc atgacaggaa cagcccgtcc cgtgctctgc
    21661 caatcgtcat acgcgagcag ttcgcgcgcg ccgtcggcgt gcatgtagta aagaccgagc
    21721 ggtggattga acgggccaaa agtcggcagg tatccttccg ggcagaacga gcagaaatag
    21781 ttttcctcat caaaggcaaa gggatgcgcg tattgcggcc cgaactgacc gaagacgtcg
    21841 atcttgaagt catggtaggg acgggcaggg tcgggcggct ctggccggat gaagtcagcc
    21901 gactcgcggc ccagcccgcc attgagccgg gtgttgacaa attcgatgcc ttgtcccgcc
    21961 tgggttcctt cggtacggtc gatcaaaccg agcttgccct tgtagatgga gtgatgcccc
    22021 gcaatcacag cgatcgcctt atgcgagccg gggataccgc gggcatgaat gattgcggcc
    22081 gggaacatgc tgttgttccc gtagtattcc gtctgcgatg tcccgtcggg attcattgcg
    22141 atcagggggt gcagatagag cgcgctgcgg tcgttatact cccagcgggt aaatacgatg
    22201 cggccgtctt ccagcacccc ggggttgctg gtcatgagct ggtcggatgt gagacggcgg
    22261 atgccgctgc cgtcaatgtt acaggtgtaa atgttgccgc ccgcgcggta ccagcaatcg
    22321 ctgatgtgta cgtcgcgcgt ggaagtgaac gcgatacgtc catccggcag gaacacgggc
    22381 tcgcagtcgg caacgtgcag cgtcttgcca tccttgacga tgggaaacgt gatctgccgc
    22441 accttgcgcg acgccacatc catcgtgtag agatagtatg agtcggtatc aaagtgatcg
    22501 cgcatcgcga acaccacggt ctgcgcgtcc caagagaggc tgggataaca gatcgcgcct
    22561 tcgggttttt cgatcagcac ctcatgtttg actgcgccgt cctcaagcaa ggttcccatg
    22621 cagagttgtc cgccccgctt gaaaccgggc gtgaaatcgc ggacctgctc gtcgggaatg
    22681 tcatatttgc ccaccagtcc ctgcatgcag ccgaaaatgc cgtgtttgac gtagatgaac
    22741 cgatcggctt tggcacggat gtctcgcagg cgcactttgc gtctcgcggc acaggcatcg
    22801 agatacagat cgcgccagcg ccggtcacgt gcgggcactc tacccgtatc aagagactcc
    22861 agccgttgcc gcaacgtctt tgcggcaggg tcatccacag gcacgcaccc cactacacgc
    22921 tgcaccatcg cgcgttcctt ggcggcatcg ttcggcgagg tgaaaacctg ggcgatgtcg
    22981 agcccggcgt cttgatagag ccagtcgcgt tcaagccggt ggcgcggaaa gg
//
LOCUS       DAOSRB010000019        22794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2443_length_22794_cov_7.419236, whole genome shotgun sequence.
ACCESSION   DAOSRB010000019 DAOSRB010000000
VERSION     DAOSRB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22794
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2443_length_22794_cov_7.419236"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /gene="dtd"
                     /locus_tag="PLZ74_02090"
     CDS             complement(<1..391)
                     /gene="dtd"
                     /locus_tag="PLZ74_02090"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOR97142.1"
                     /translation="MKALIQRVAEASVEVEGRRVAAIGRGLLVLLGVTHGDTLKEVAW
                     LARKIAALRIFEDEQGLMNRSVSETGGAVIVVSQFTLYADTRKGNRPSFTEAARPEQA
                     EPLYRELVRQLRLTLGADRVGTGVFGAE"
     gene            634..1989
                     /locus_tag="PLZ74_02095"
     CDS             634..1989
                     /locus_tag="PLZ74_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR97143.1"
                     /translation="MRVRGITRRKFLSGGTGAGAALICGRTFAEGEAAGLAELIRALG
                     WDPNAPGSFFFLQATDLHASEITSGALKMPDKFGGRNFADDMNALRPAPAFVALTGDL
                     IAATTRNPATWPQAEAGFRRVRDLIVSRLTVPCHMIIGNNDCSPEAYHAVWPDRPLYW
                     SFDRQGVHFVGLHGYNTWKPENTNHAGILLDDSQLVWLKRDLEAAANAKTLVLFTHEA
                     LKDPDCHRIRKQLAPLLAAFPGEIWNIAGHNHGNAECEIRLGGKPVRVVETITPVGAW
                     TPDKGAYRLVFVSNGRVVGSGLRWLTKEGVPLRFEAVASDRNIPTRTLIETALAEGAH
                     RVFMVGEEDLPLRLDTTGVTDRISNFQFRKGAALTYRLPLTLRGKDATRLRLACGAKV
                     SVTFSADGTVWHPASKQAHAAGVVTVDVPEALRATANLTVRIQAPPDQGARLYGIAVL
                     R"
     gene            complement(2118..4220)
                     /locus_tag="PLZ74_02100"
     CDS             complement(2118..4220)
                     /locus_tag="PLZ74_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR97144.1"
                     /translation="MLFRTILFCCAFALLAVCRGQTNTPVDLGTFVVEGTPISKYRAE
                     TVSTATFADAKPEELPQTVEVLTEDFISEMNPVDLHDMLRHQAGVYTGGKSMLDRTSG
                     QYTLRGMSGSDVMLNGTLGLAGPMGTFMDPSAFERIEVVKGPVGSTVGGASSTLGPYG
                     AGGSVNLVMKQPRDDQDFLHLKPRASLGEHSQRYRLPFDINERVIEDRLTVRLPGNIE
                     YGRPFWLSSGYDWRESYYLAPSVFMAVRDDLRIGANLSIQYTDQPAYQGIPVYKGKPY
                     AGYDWDSNPALSHMRDTYIGHTLDAYVEWDASRVWTLRSGAGVAQADIEFEHLGPAAF
                     ANASTDALLPLPLAKPYDHQEGDMLYRRYNAYQRATAVFDTGPVGHQAVFQGDFSRKT
                     SKGRSYFESVAKPDAVHRWVAADYQNTELDKYGFLAQDYLSWWKLRVLVGGRFDDHES
                     DQGNSANAFCPRGGISFLPTDWLVFFANVSYTEAPNFGYNGKDNRELTSTWEAIQYET
                     GFRVAPVKTLWLSAALYRIDQDNTPIALPEDRTRYTEEGANESQGVEVSLTGNISDRW
                     SVYASYAYNEYENKQQKKRFDRYPPHAATLSTSYRLAGGPLDDIVLGFGYRYRHRYAA
                     TMRGEYVGDNFYIDDSHVFDCSADIPLYKFGGPKNVTLSLACKNIFNRHYIESTRHYY
                     QCFPGDPRTFELALQAEF"
     gene            complement(4310..5035)
                     /gene="rph"
                     /locus_tag="PLZ74_02105"
     CDS             complement(4310..5035)
                     /gene="rph"
                     /locus_tag="PLZ74_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOR97145.1"
                     /translation="MKKTPARLRPVSFERRFTRHAAGSVLIRQGDTWVLCTASVEEKV
                     PPFLRGSGKGWVTAEYSMLPGSTIERKEREIRRGKPDARGMEISRLIGRALRAAVDLE
                     RLGERTITIDCDVLQADGGTRCASVTGGMVALADALTALVRQGKLSSPPLRHFVAAVS
                     VGICGGKPVLDLDYAHDSVAEVDLNVVMTDDGRYVEVQGTAEGEPFDEAVLDTLRSRA
                     RKGIRDLIRMQKAALREEPPHHP"
     gene            complement(5032..6546)
                     /locus_tag="PLZ74_02110"
     CDS             complement(5032..6546)
                     /locus_tag="PLZ74_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOR97146.1"
                     /translation="MNVWIRTAGVGVFWAGLLWAGDPVPYGAIPSERQVRWHEIEVFS
                     LIHYGLNTYTDREWGYGEEDPALFNPANLDANQIVSALRSGGMRGVILVAKHHDGFCL
                     WPTQTTAHNISKSPWRGGKGDLVREVADAAKKHGLHFGVYCSPWDRNHAAYGTPAYLA
                     VYQAQLRELLSQYGTLFEVWFDGANGGDGYYGGAREKRSIDRFTYYDWPNTWKIVREL
                     QPSACIFGDVGPDCRWVGNEKGYAAEESWATFTPKGKTDPARPANGDSMNHIEGPAGH
                     RNGTAWMPAECDVPLRKGWFWHPDQDATVRSPATLLDIYFRSVGKGGCMNLGVAPDRN
                     GRVCEADTRSLEAFGNLLSVLFAVNFAEQATVSASHVRAWEPAPGKTHPYEPARVVDR
                     DRYSYWATDDGVTQATLTLTLPAAVEFDVVRLRENIRLGHRVDGFVVEVWQGQAWKEY
                     AKGQSIGACRLIRGSKVQTDRVRLRITSAAACPCISDFGLFALPATLAPSLPKP"
     gene            6764..7273
                     /locus_tag="PLZ74_02115"
     CDS             6764..7273
                     /locus_tag="PLZ74_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97147.1"
                     /translation="MKRMWSGLVLACLIAVGAQAAEKVRWPVWCAFNDTEDIDVVGLR
                     LNLPSGQCEQVTGLDIGLIGRSQYFNGVQINLLRNSVLDALAGWQIGCYNSTGLGDML
                     GMQTGLWNESRTLYGLQVGLINLADYAEGFQVGLVNRAEDMHGYQIGLINIIRGGDVP
                     FCPLINIGF"
     gene            7358..8578
                     /locus_tag="PLZ74_02120"
     CDS             7358..8578
                     /locus_tag="PLZ74_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOR97148.1"
                     /translation="MLIKPFAALRPAPDRAASLASVPYDVVDTAEARALAAGNPDSFL
                     HVSRPEIDLPDTVDIYDDAVYAQGARAFAELQARGVLLREAEERIYVYRQIMGTHSQT
                     GVVACCHIDDYARDVIRKHEKTRQDKEDDRTRHCLELNANSGPVFLTYRTREDLDARV
                     AQAQQATPLYDFTAADGVRHTVWRAEDDPAAWVEAFHHVPLAYVADGHHRAAAAFRAG
                     KQRREAHPGHTGHEEYNWFLAVLFPADQLRILPYNRCVADLNGLTPDAFLERVRTLFR
                     TEPTDGAEPAGPREIRMFLNGAWHRLTWEAVDADPVGRLDVSVLQNRLLGPVLGIDDP
                     RTNTRIAFVGGIRGTGELVQRVTSGQDAVAFSMFPVTVQQMMDIADAGQIMPPKSTWF
                     EPKLRSGLLVHTLD"
     gene            complement(8606..9115)
                     /locus_tag="PLZ74_02125"
     CDS             complement(8606..9115)
                     /locus_tag="PLZ74_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97149.1"
                     /translation="MWVVLAPLLLQARVGENLKQSETRYGTGEELQDAETFILKNARN
                     VVYFHGDWIITAGYLNNVTARIQYEKAGLRQPRAQLTREDVAEILEAEDAAGWSAYNE
                     ETRQAGKAYTGKAHPPPVLRSVSGLTARYKVFSVTVEREEASHHEKGTTLRQPLRPTL
                     TPRGKRVAL"
     gene            complement(9195..10652)
                     /locus_tag="PLZ74_02130"
     CDS             complement(9195..10652)
                     /locus_tag="PLZ74_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97150.1"
                     /translation="MKSMLRWFIGIGLAGVLFAEEKSPAGQGAGAAAPADVAQMKARE
                     TGGLDWDAGADLRVRQEASDNLPKRINAQGEGVTAKNDNYLRIRPRVWGQVKNEDFKL
                     YLRVADEFREYFQPRESRNARPPDEVLVDSLYLDLYNLADGWLDLRIGRQDFFGPDGP
                     VYGAGRVICDGTPFDGSRTIFMDAIKATVKFDEKNSLDLLAIYNSAETEMSWGHPYRT
                     DGSEYKERPLTSIHPASKDMDEYGGGLYFRSRSVPEVPFELYAFYKRETKARLAGGAR
                     LPGRNTYTWGLRVMPQLTETLSAEVEGAVQAGEKDGGASTSGYMGYAGLTYRPLIDGS
                     SKPFFTGSLYYLSGDKNRDAEGDNDTAWNPLWARWPQFSEMYVYSFMYGVGYWSNLLY
                     PSLEAGVGFAPGHKVRASVGPMYVAVEDDLGGGNGDLLGWLGVVRYDFPLFKNIFGKR
                     GDLTGHVTAEVLDPGDYYVSDTVAYFLRWEVIARF"
     gene            10835..11494
                     /gene="plsY"
                     /locus_tag="PLZ74_02135"
     CDS             10835..11494
                     /gene="plsY"
                     /locus_tag="PLZ74_02135"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOR97151.1"
                     /translation="MTTVTVWIVGGVAAYLLGSIPFGFLLARSRGKDIRTLGSGNIGA
                     TNVFRSVSKPLGVLTFALDFLKGLGGVTLIPALALTICPPETGAAGGKGLAMFCGALT
                     VVGHNWTCFLRFKGGKGVATSTGLVLGLAPQAVGIAFVVWLVVFLMTRMVSAASVAAA
                     VTLAAAVWWPLRLHAAYGFWFPAVFTALAAVAVWKHRSNLARIRNGTEARFTFRTRRQ
                     V"
     gene            11521..12510
                     /locus_tag="PLZ74_02140"
     CDS             11521..12510
                     /locus_tag="PLZ74_02140"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOR97152.1"
                     /translation="MHITIIGDGGWGTAVGLLLNGYGHAVTLWGPFPEVVDEIRERRE
                     NTRFLPGIPLPETLRWTADAAEAVAGADVVILASPSKFLAQVCERFKGLIPADCLTVS
                     LTKGLCETTHCRMSQLARTILGLRSIVVLSGPSHAEEVARRLPTAVVAASEEEELTRR
                     VQQLFSGPAFRVYTSPDPLGVEIGGAVKNVIAIAVGVSDGLGFGDNSRAALITRGLAE
                     ITRFGTALGACPATFSGLSGAGDLIVTCTSRHSRNHMVGERLGRGERIQEILDSMKMV
                     AEGVWNAKIIHALSRTHGVSMPITEVVHALCYEGLSARDAVEALMTREVKPEQ"
     gene            12619..13701
                     /locus_tag="PLZ74_02145"
     CDS             12619..13701
                     /locus_tag="PLZ74_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97153.1"
                     /translation="MKRKRVLHIALIYNTYRDAVPEAPDDRGSTNDLRRFIRSLARAL
                     RSLGHRVTVLPLANDMLGFQRKLARLQPDVVFNQYDDVVHSALYEMRVAALVRMMGFP
                     ITGSHALALGLCRYKHMAASLLQGAGLPVPPDARLLERVKDVSGVKWRFPLIVQAAKE
                     HAGVGLTRHSVVHSVTALRRQVGIILRDYHQPATVQRFLPGREFNIGIIGGAKQRVLP
                     FAEVNYAGLPPGIPPIMSYDAKWTESSIEYKCTSVICPADVAPPLARKLTNLTVKAFR
                     ALGGWGYGRVDIRLDEAGEPHVLEVNCNPCLEEDVALARSAKAAGIAYPQLLQQILDA
                     AFEKPPFDTDVPMLPPPRPQNPLRPT"
     gene            complement(13748..15262)
                     /locus_tag="PLZ74_02150"
     CDS             complement(13748..15262)
                     /locus_tag="PLZ74_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97154.1"
                     /translation="MKTVWKTSRGWGAIALSAWVLCVRGAIVDPADYGFSPDAPAATN
                     AVALQRALDGGKRTVQVTRPGVYGLDRTVWLDSDTRLIFTPGAVLQKQAAYVHVLANR
                     GLESWAWDTNIVVEGATIDVNDFSAGHGEDSPQYGNRGQISFCRIRDLTVRKFTCLNL
                     HGGQYGFQIATFENVILDEFEIRGAKDGVHVNAGRKFVIRNGILETGDDGIALTAMDW
                     VSSAPVVGDIEDGLIENITDDGRGRCNFSRILTGAWTDWHKGIRLQRGDTVRCGKKLY
                     RVAMPRGTNEYVSLEAPTHTRGIWTDATGLKFWFMQDSDALAANIRNVTFRNLYLRSP
                     RNGFLADWETRSSWNRGIHPEVKPENYPVCEVRLEDVYSESPGALVSGYESVRLWLRN
                     ITKTRGPLVNLARPNDNRDCAIDVWLHLSGVVLKADDGKGADISVGGAGSSLNLTVEG
                     LMQERDLRVSAGAGVRARVNGIGSIHSLEGLTPEAGDSIKVGGVLKTFDGTQWR"
     gene            complement(15318..16559)
                     /locus_tag="PLZ74_02155"
     CDS             complement(15318..16559)
                     /locus_tag="PLZ74_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97155.1"
                     /translation="MKKECFSLCLTVVAAVVVGLDARAANIEFYRRSKTGGSWNDVNH
                     WVTNAGGSVLMGTYPKDPATDRVWFKDGFTNDTQELYLESDINVLNMMQLNASAGNQT
                     NYLNLGGHTLYVVNFLNQDYGCVGTFAITNGNISSMMIRTIGSGYCLWFKGPNTRFDL
                     HGDWYRICIGYNSARSTLRVSDGAVCNWPCTMFYLGGEQLSGKHDGNRLVIDSGGLVT
                     NCNTDLVINRGGGTNGLYVSDGGKFHSNAGIQMNSSDGGWHTNPVMKSVTAILDVRDG
                     TVDIRRLTGSVGSNSVLRLAGPQSDLRIRNEMVITNTFDMTFDMTDGVFAAETAPIRL
                     TSTSHTHILGDGTIRVRYPQRFAKEHKGLIPLIHAASGNLTYTGDIRFDFTEARWAQA
                     VTNSTMVAIKIVPPGLVFSIQ"
     gene            complement(16599..16940)
                     /locus_tag="PLZ74_02160"
     CDS             complement(16599..16940)
                     /locus_tag="PLZ74_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97156.1"
                     /translation="MNRCAVACGCLALVQATFAGSATWNYREGAGGSPANPTLWTDTA
                     NWTGGAVRLTGDTSKFTTGSYKLIASSGLIPGSEWTVDGYTGRKVLKLRVKADGLYLE
                     VIEQGLSVVIR"
     gene            complement(17563..18549)
                     /locus_tag="PLZ74_02165"
     CDS             complement(17563..18549)
                     /locus_tag="PLZ74_02165"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOR97157.1"
                     /translation="MITNATRRGFIKTAGAAAGTAFLPRLAAGQASRTTPKLVLIAGE
                     RSHGYMAHEHYAGFVLIKKWLEASGIGLQAVVCKNGWPQDESVFDGATALAVNSDGEG
                     RHPLNGHLEKLDELRKRGVGLALLHFALIINKGPLADRALAWCGGYDEAHWSVVKWWK
                     AEFKTLPVHPVTRGVTPFAFYDEWYFHMRFRPETAGITPLLSAVPPDEVREGKDGPHS
                     GNPVVRSRKGAIEHVAWASECPDGTRGFGFTGLHNHWYLGHPQFRKVLLNGLAWCAGA
                     EIPKNGIESPQPTLDELKRNQDKPVPPNFDFGKIQKELNRWKEPTQTSETRG"
     gene            complement(18596..19966)
                     /locus_tag="PLZ74_02170"
     CDS             complement(18596..19966)
                     /locus_tag="PLZ74_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97158.1"
                     /translation="MTTVSRRRFVAASSLAAFGFRIVPRHVLGAPGQPPPSERLNLGL
                     IGIGGMGKSHAGIFSTIPSVRIAALCDVDMTRATDVLKQYPDLPFHKDYRVMLEKEKG
                     LDAVAIATPDHTHAPISLLAMKLGKHVYCQKPMAHTIEEARLMANVAAETKTVTQMGN
                     QGHAGEGLRLTKEWLDAGVIGAVKEIHVWTDRPIMNMGAKAVRVWYSNGQRRPTATPP
                     VPASLDWNLWLGPAPEHAYHPDYVPQVWRPWFDYGNNAIGDMMVHNADPAWYALDLGA
                     PDAVEALTSEKNPDSFPVWNVVTWEFGANAKRGPIKLTWYDGGKTPPPPPGMEPDRKL
                     DHNGIYFVGEKGVILCGGWAGAPRLVPESAMKTFERPPKTIPRNRGGHYGEWVAASIA
                     GRPQDAQAGFWWSGPYMESLLVGTLAIRLNKRIEWDGAAMRAKNAPEADPLIRKAYRK
                     GFELPV"
     gene            complement(20069..21304)
                     /locus_tag="PLZ74_02175"
     CDS             complement(20069..21304)
                     /locus_tag="PLZ74_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOR97159.1"
                     /translation="MMTRLYVRSLRALALLLACWGLGVATAVAQNAPAAVDAKRHQAA
                     KPFVGFERGMGIGGWLTNYKRFNVLPDKWKMTLSPGDYEHFETYITKNDVQNIASFGV
                     DHIRLGFDQIVLEEKPGVYRERTFRCIDNFIGWCREYKLNVVLNLHKAVGNYCDVPEP
                     VALLDDAGLQNRFVALWLEFERRYHADNRIVFELLNEVKNVDPQAWNTLAARTISEIR
                     KLNPTRKIVIGSTCWNNCHKLKDLQVFDDENVIYTFHFYDPFGFTHQRGVLQAGPLYY
                     NRAMPYPGDIERYRDYKRFVEGAKNPYPRYQRMDIKVLREWLQPAFDFAKKHPDKILW
                     CGEFGTIRHCDMTSRENWMNDVILLLKEHGISYCVWNYLSTPNDGNRFSLVDDEKRHI
                     LSPRMLAIIQGRVEPLREE"
     gene            complement(21383..21613)
                     /locus_tag="PLZ74_02180"
     CDS             complement(21383..21613)
                     /locus_tag="PLZ74_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97160.1"
                     /translation="MLGHALDQQGPWAGRRTNVTWVWMIETLRVVIGQIDWSTARQDV
                     QRFLPLREQESLGLWGSEFFLYYADMMETMAP"
     gene            complement(21594..21983)
                     /locus_tag="PLZ74_02185"
     CDS             complement(21594..21983)
                     /locus_tag="PLZ74_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97161.1"
                     /translation="MAREAVYRVSSVTVARSREFVTPLGVFSFQRVPALVPRAGVEAV
                     EVARNAWAFIASPLRAIADLIYLNKPITWKRNGMSYLTESLRIEEEDLQTLSFEALDE
                     ILESLRSRRVYAYLQGLKGACLAGACA"
     gene            22448..>22794
                     /locus_tag="PLZ74_02190"
     CDS             22448..>22794
                     /locus_tag="PLZ74_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HOR97162.1"
                     /translation="MPRMKTKTADSAASLGFEAKLWLAADKLRNNMDAAEYKHVVLGL
                     IFLKYISDAFEAMRQKLTEGKGEYKGANPEDPDEYRAENVFWVPPAARWSYLQGSAKQ
                     PTIGRIVDDAMTA"
BASE COUNT         4394 a         7310 c         6688 g         4402 t
ORIGIN      
        1 tctccgcgcc gaacacaccc gttccaacgc ggtccgcgcc cagagtcagg cggagttgcc
       61 gcaccaattc gcggtacagc ggctcggcct gctccggccg cgcggcctcc gtgaaactcg
      121 ggcgattgcc tttgcgcgtg tcggcataca acgtgaactg cgagaccacg atcaccgcac
      181 cgcccgtctc cgagacagac cggttcatca ggccctgctc atcctcaaag atgcgcagcg
      241 ccgcaatctt gcgtgccagc cacgcgacct ccttgagcgt gtcgccatgc gttaccccaa
      301 gcagcaccag caggccgcgg ccgattgcgg ccacacgccg tccctcgact tccaccgaag
      361 cctcagcgac tctttggatc aacgctttca tccgaatcgt tacccgtaag cataacaccg
      421 ttcatgcaca cgcttcaacg taagaacgga gacgcgaaaa aaatgaaaca catgaacctt
      481 catgcaagca agacgggagg gcgtcggaaa cgcgggtgcc cgcgcccgca acttggtttc
      541 atccgcgcat ggacacatcg gatcggaaca tggtatgctc cgaatcaaga ccatcctgtc
      601 tgacaaatct gagcggggtt agggagaaga gcgatgcgag tcagaggaat cacgcgccgg
      661 aagtttcttt ccggcgggac gggcgcgggt gccgcactga tctgcggacg tacgtttgca
      721 gagggagagg cggcaggact ggcggaactg atccgggccc tagggtggga tccgaacgcg
      781 ccgggaagtt tcttttttct ccaagccaca gatctgcatg cgagtgagat cacatccggt
      841 gctttgaaaa tgccggataa attcggaggc cgcaacttcg cggatgacat gaacgcgctg
      901 cggcccgcgc ctgcgttcgt tgcgctgacc ggagatctga tcgccgcgac cacacgtaac
      961 cctgccacct ggccgcaggc cgaagccggt ttccggcgcg tgcgggatct gatcgtctca
     1021 cgtctcaccg tgccctgcca catgatcatc ggcaacaacg actgctcgcc cgaagcctat
     1081 cacgcggtct ggccggatcg tccgctctac tggtcgtttg acaggcaagg cgtacatttc
     1141 gtcggcctgc acggatacaa cacatggaaa ccggagaaca ccaaccatgc cggcatcctg
     1201 ctggatgatt cgcaactcgt ctggttgaag cgcgatctgg aggccgccgc caatgcgaag
     1261 actctggtgc tcttcacgca cgaagcgctc aaggatccgg attgccaccg tatccgcaaa
     1321 cagcttgccc cgcttctggc cgcgtttccc ggcgagatct ggaatatcgc gggacacaac
     1381 cacggtaatg ccgagtgcga aatccgcctc ggcggcaaac ccgttcgcgt agtcgagacc
     1441 attacacccg tcggggcctg gaccccggac aagggtgcgt accgacttgt cttcgtgtcg
     1501 aacggacgtg tcgtgggttc agggttgcgt tggctaacca aggagggcgt gccgctgcgg
     1561 ttcgaggccg tggcatcaga ccgcaacatc cccacccgga ccctgattga aaccgcattg
     1621 gctgaaggcg cgcaccgcgt ctttatggtc ggcgaggaag atctgccttt acgtctcgac
     1681 acgacggggg tgacggaccg catctcgaat ttccaattcc ggaaaggggc ggccctgacc
     1741 tatcgcctgc cgttgacgtt gcgggggaag gatgccacgc gcctgcgcct cgcctgcgga
     1801 gccaaggtga gcgtaacgtt ttcggctgac ggcacagtct ggcacccggc ttcgaaacaa
     1861 gcgcacgccg caggagtcgt caccgttgac gtgccggagg cgttacgcgc cacggcgaat
     1921 ctgactgtgc gcattcaagc gccgccggat cagggcgcgc gactctatgg gatcgctgtg
     1981 ctccgctaga gtccgggatc tgccgagcgg gaaaaccgac aggtattttg ggccttcttt
     2041 ggttccgggt ttcacgcatc acccgaatca tgcgcgagcc tgatcgcgca aaacgttcgg
     2101 ttgaccgaaa tgcaggctca gaattccgct tgaagcgcca actcgaatgt gcgggggtct
     2161 ccgggaaaac attggtaata gtgtcgggtg gattcaatgt agtgcctgtt gaagatgttt
     2221 ttacaggcga gggatagcgt cacatttttc ggcccgccga atttgtacag ggggatatcc
     2281 gccgagcagt cgaagacgtg cgagtcgtct atatagaaat tgtcgccgac atactcgccg
     2341 cgcatggtgg cggcgtagcg gtggcggtaa cggtaaccga agcccagcac gatgtcatct
     2401 agcggcccgc cagccagacg gtaggacgtg gacagcgtcg ccgcgtgcgg cgggtagcgg
     2461 tcgaagcgtt ttttctgctg tttgttctcg tattcgttgt aggcgtatga ggcataaacc
     2521 gaccacctgt cgcttatgtt accggtgagc gagacctcca cgccctgcga ttcgttggcc
     2581 ccctcttcgg tgtagcgggt gcggtcttca ggcagggcga tgggggtgtt atcctggtcg
     2641 atgcggtaga gcgcggcaga gagccaaagg gtcttaaccg gcgcgacgcg gaaaccggtc
     2701 tcgtactgga tagcctccca tgtggaggtc agctcgcggt tgtctttacc gttgtaaccg
     2761 aagttcggcg cttccgtgta cgagacgttt gcgaagaaaa ccagccagtc ggtcggcagg
     2821 aacgagatgc cgccacgcgg gcagaaagcg ttggcggagt tgccttggtc gctttcgtga
     2881 tcatcaaaac ggccgccgac cagcacacgt aatttccacc aggagagata atcctgcgcg
     2941 aggaaaccgt atttgtccag ctcggtattc tgatagtcag ccgccaccca gcggtgtacg
     3001 gcatcgggct tcgcgacaga ttcgaaataa ctgcggcctt tagatgtttt gcgcgagaaa
     3061 tcaccctgaa aaaccgcctg gtgcccgacc gggccggtat cgaaaaccgc cgtggcgcgc
     3121 tggtaggcgt tgtagcggcg gtacagcatg tcaccctcct gatgatcata gggcttggcg
     3181 agcggcagcg gcagcaacgc gtcggtggag gcattcgcga acgcggcggg gccgagatgc
     3241 tcaaactcaa tatcggcctg cgcgacgcca gcgccactgc gcaaggtcca gacgcgcgaa
     3301 gcatcccact ccacataggc atcaagggta tggccgatgt aggtgtcgcg catgtgcgac
     3361 agcgcgggat tggaatccca gtcatatccg gcgtaaggct tgcccttgta gaccggtatg
     3421 ccctgataag cgggctggtc ggtgtactgg atcgagagat tggcgcctat gcgcagatca
     3481 tcgcgcaccg ccatgaacac agagggcgcg agatagtacg attcgcgcca gtcatagccg
     3541 ctggagagcc agaacggacg gccgtactcg atattgccgg gcaggcgcac ggtcaggcgg
     3601 tcttcaatca cgcgctcgtt gatatcgaac gggaggcggt aacgctgact gtgctcgccg
     3661 agggaggcgc ggggtttcag atggagaaag tcttggtcgt cacgcggttg tttcatgacc
     3721 aaattcaccg agccgcccgc gccatagggc ccgagtgtgg agctggcgcc gccgaccgtc
     3781 gaaccgaccg gccccttgac gacctcgata cgttcgaagg ccgaagggtc catgaatgtg
     3841 cccatgggcc cggccaggcc gagcgtgccg ttgagcatga cgtcgctgcc ggacatgccg
     3901 cgcagcgtgt attgtccgga agtgcggtcc agcatggact tgccgccagt atagacaccg
     3961 gcctgatggc gcagcatatc gtgcagatcc accggattca tctcgctgat gaagtcttcc
     4021 gtcagcacct ccaccgtctg cggcagctct tccggtttcg cgtcagcgaa ggtggcggtc
     4081 gaaacggttt ccgcccggta cttcgatatc ggggtgccct ccacaacaaa ggttcccaga
     4141 tcgactggcg tgttggtttg gccgcggcag acggccaaca gggcgaacgc gcagcaaaac
     4201 aggatggttc ggaacagcaa gatcggatct cctcgtttga tgggtgacgt ttccacctct
     4261 ttcaaacgag agcgtcttta ccacggttca tcggaaagaa agaaaaacgt tacggatggt
     4321 gcgggggctc ctcacgcaac gcggccttct gcatgcggat caaatcgcgg atccctttgc
     4381 gcgcccgaga gcggagcgtg tccagcacgg cctcgtcaaa cggctcgccc tcggcagtac
     4441 cctgcacctc cacataccgc ccgtcgtcgg tcatgaccac attgaggtcg acctccgcaa
     4501 cgctgtcgtg cgcataatcg aggtcaagca cgggcttgcc gccgcagatg ccgacgctga
     4561 ccgctgccac gaaatgccgg agcggcgggg aggagagctt gccctgccgc accagcgccg
     4621 tcaatgcatc ggccaacgcc accatcccgc cggtgacgga agcgcagcgc gtgccgccgt
     4681 ccgcctgaag cacgtcgcaa tcgatcgtga tggtgcgttc gccgaggcgc tccaaatcca
     4741 cggccgcgcg gagtgcgcga ccgatgagcc gactgatctc catgccgcgc gcatcgggtt
     4801 tgccgcgccg gatttcgcgc tccttgcgct cgatcgtgct gccgggcagc atgctgtact
     4861 ctgcggtgac ccagcccttg ccactgccgc gcagaaacgg gggcaccttc tcctccacac
     4921 tcgcggtaca gagcacccaa gtgtcgccct gacggatcag gaccgaccct gcggcatggc
     4981 gggtgaagcg gcgttcaaac gaaacggggc gtaaccgggc aggcgttttt ttcatggctt
     5041 cggaagtgaa ggcgcaaggg tcgcgggcag ggcgaagagg ccaaagtcac tgatacaggg
     5101 gcaggccgcc gccgaggtga tacgcaaacg aacgcggtcc gtctgcacct tggagccgcg
     5161 gatcagacgg caggcaccga tactctgccc tttggcgtat tccttccatg cctgaccctg
     5221 ccacacctcc acgacgaacc cgtctacgcg gtgccccagc cgaatgttct cgcgcaggcg
     5281 caccacgtca aactccaccg ccgctggcaa ggtcagggtg agcgtcgcct gagtgacgcc
     5341 gtcatccgtg gcccaatagg aataacgatc acgatccacg acacgggccg gctcataggg
     5401 atgcgtcttg cccggcgcag gttcccaggc gcgcacatgg ctggcgctga ccgtggcctg
     5461 ttcggcgaaa ttcacggcaa aaagtacgct cagcaggttc ccgaaggctt cgagcgaacg
     5521 ggtatccgct tcgcagacgc gtccgttgcg gtcaggcgcc acaccgaggt tcatgcaacc
     5581 gcctttgccg acactgcgaa agtagatgtc cagtaacgtg gcgggcgaac gcactgtggc
     5641 gtcctgatcc ggatgccaga accatccctt gcgcaagggc acatcacact cggccggcat
     5701 ccaagccgtc ccgttacggt ggccggccgg accctcgata tggttcatgg agtcgccgtt
     5761 ggcggggcgg gccggatccg tcttgccttt aggggtaaag gtcgcccacg actcttcggc
     5821 ggcgtatccc ttctcgttac cgacccagcg gcaatccgga ccgacatcgc cgaagatgca
     5881 ggccgagggt tgcagttcgc gaacgatctt ccaggtgttg ggccaatcat agtaggtgaa
     5941 acggtcgatg cttcgttttt cacgcgctcc gccatagtac ccgtcgccgc cgttggcacc
     6001 gtcgaaccag acctcgaaga gcgtgccgta ctgggaaagc agttcgcgga gctgagcctg
     6061 atagaccgcc agataggcgg gcgtgccgta ggccgcgtga ttgcgatccc aaggcgaaca
     6121 gtagactccg aaatgcaacc cgtgtttttt ggcggcgtcg gcaacctccc gcaccagatc
     6181 gcccttgccg ccgcgccaag gggatttgga gatgttgtgc gctgtggttt gggtaggcca
     6241 gagacagaaa ccgtcatggt gtttcgccac cagaatcacg ccccgcatcc caccgctgcg
     6301 gagcgcggac acgatctggt tcgcatcgag attcgcggga ttgaacagag cggggtcctc
     6361 ctccccgtat ccccactcgc ggtccgtgta ggtgttgagc ccgtaatgaa tcagagaaaa
     6421 aacttcgatc tcgtgccacc gtacctgtcg ttccgaagga atcgcgccat acgggaccgg
     6481 gtctcccgcc catagcaacc ctgcccagaa cacgcctacg cctgctgtcc gtatccacac
     6541 attcatgtcg cctctcccgc attttgaatt gtcattatct gtcatcttaa cgagtatgag
     6601 gcattccggc aagccgcaaa ccgcataaaa aaatcgcgcc catcgcattc tggcctttgc
     6661 gccgcaccct aaaatctgta tagttaataa ccatacgcgt tcgggtgtcc gttgatccag
     6721 cggacaagac gatgtcgggt tcattagcga ggcgaggtaa ttcatgaaaa gaatgtggtc
     6781 tggtttggtt ctggcttgtc tgattgccgt gggcgcgcag gctgcggaaa aagtgcgttg
     6841 gccggtttgg tgtgcgttta acgacacgga ggacattgac gttgtgggat tgcgcctcaa
     6901 tctgccttcc gggcagtgcg agcaggtcac cggtttggat atcggtctga tcggacggtc
     6961 gcagtatttc aacggtgtcc agatcaacct gctgcgcaac agcgtgctgg acgcgctggc
     7021 cggctggcag atcgggtgtt acaactccac ggggctcggg gacatgctcg gaatgcagac
     7081 cggcctctgg aacgaatccc gcaccctgta cggactgcaa gtcggactga tcaacctcgc
     7141 ggattatgcc gagggtttcc aggtcggatt ggtcaaccgg gcggaggaca tgcacgggta
     7201 ccagatcggc ctgatcaaca tcatccgcgg cggcgacgtt ccgttctgtc cgctgatcaa
     7261 catcggtttc tgaacgggcc cgctgcccat cctgtgaagc gcaaaggacg gcccggagcc
     7321 acggtccttt gcgtttcgcg tatccggaga gtcttccatg ctgattaaac cctttgccgc
     7381 gttgcggccc gccccggacc gggcggcgtc gctcgcctcc gttccgtacg atgtggtcga
     7441 caccgccgaa gcgcgcgcgc tggccgccgg caatccggac agttttctgc atgtgtcgcg
     7501 gcccgagatc gacctgcccg acacggtgga catctatgat gacgcggtct atgcgcaggg
     7561 cgcgcgggcc tttgcggagt tgcaggcacg cggggtgctg ttgcgcgagg ccgaagagcg
     7621 gatctatgtg taccgccaga tcatgggcac ccacagccag accggcgtgg tggcctgctg
     7681 ccacatcgac gattacgcgc gcgacgtgat ccgcaagcat gagaaaaccc gtcaggacaa
     7741 agaggatgac cgcacacgcc actgccttga actgaacgcc aactcgggac cggttttcct
     7801 gacctaccgg acgcgggagg atctcgacgc ccgcgtggcg caggcacagc aggccacacc
     7861 actctatgac ttcaccgcag ccgacggggt acgccatacc gtctggcgcg ccgaggacga
     7921 tcccgccgcg tgggtcgaag ccttccacca cgtgccgctg gcctatgtgg cggacggcca
     7981 ccatcgcgcc gcagcggcct tccgcgccgg gaaacagcgc cgcgaggccc atcccggcca
     8041 taccgggcac gaggagtaca actggtttct cgccgtgctc ttcccggccg atcagttgcg
     8101 catcctcccc tataaccgtt gcgtggcgga cttgaacggt ctgaccccgg acgcgtttct
     8161 ggagcgggta cggacgctct tccgtaccga accgaccgac ggtgcggagc ccgccggacc
     8221 gcgtgaaata cggatgttcc tgaacggcgc gtggcaccgg ctcacctggg aggccgtgga
     8281 cgccgatccg gtggggcgac tcgatgtgag cgtactgcag aaccgcctgc tcggccctgt
     8341 gctcgggatc gatgacccgc gcaccaacac gcgcatcgcc tttgtcggcg gcatccgcgg
     8401 caccggcgaa ctggtccaac gcgtgaccag cggacaggac gccgtggcgt tctcgatgtt
     8461 ccccgtgacg gtgcagcaga tgatggacat cgccgacgcc ggccagatca tgccgcccaa
     8521 aagcacctgg ttcgagccca agctgcgctc gggcctgctg gtacacacgc tggactgacg
     8581 cggcacggcg accgccgcgg cacagtcaca gggccacgcg tttgccgcgc ggggtcagcg
     8641 tgggccggag cggctgccgc agcgtcgtcc ccttctcgtg gtgcgacgcc tcttcgcgct
     8701 ccaccgtcac gctgaacacc ttgtagcgtg cggtcaaccc cgagacggat cgcaacaccg
     8761 gaggcggatg ggccttgccc gtgtaggctt tgccggcctg acgggtctcc tcgttgtagg
     8821 cggaccaccc cgccgcatcc tcggcctcga ggatctcggc gacatcctcg cgggtgagct
     8881 gcgcccgtgg ctgccgcaag cccgctttct cgtattggat ccgtgcggtc acgttgttga
     8941 gataccctgc ggtgatgatc cagtcgccgt ggaagtagac gacattgcgc gcatttttaa
     9001 ggatgaacgt ttccgcgtct tgaagttcct ccccggtgcc gtagcgcgtc tcgctctgtt
     9061 tcagattctc gcccacccgc gcctgaagca acaggggcgc aagaaccacc cacatcaaac
     9121 aaggtatctt ggtttgcata acggagattc tagcatccct gcgggaaaaa cgcatctgca
     9181 aggtcgcggt tttttcagaa acgggcgatc acttcccaac gcaggaaata ggcgaccgtg
     9241 tcggatacat agtagtcgcc cgggtcgagc acttccgccg tgacgtgacc ggtcagatcg
     9301 ccgcgtttgc cgaagatatt cttgaacagc gggaagtcgt aacggaccac ccccagccat
     9361 cccagcagat cgccgttgcc gccgcccaga tcgtcctcca ccgccacata catcgggccg
     9421 acgctcgcgc gcaccttgtg acccggcgcg aagccgaccc ctgcctccag gctgggatag
     9481 agcaggttgg accagtaccc gacaccgtac atgaaactgt agacgtacat ctcgctgaat
     9541 tgcggccacc gcgcccaaag cggattccag gcggtgtcgt tatccccctc cgcatcgcgg
     9601 ttcttgtcac cggaaagata gtagagcgag ccggtgaaga acggtttgga cgaaccatcg
     9661 atcaatggcc gataggtcaa ccccgcatac cccatgtaac ccgaagtcga cgccccgccg
     9721 tctttctcgc ccgcctgaac ggcgccctcg acctcggccg aaagcgtttc ggtcagttgg
     9781 ggcatcacgc gcagacccca ggtgtacgtg ttgcggcccg gcaaacgcgc cccgcccgcc
     9841 aggcgggcct tggtctcgcg cttgtagaaa gcgtacaact cgaacggcac ttccggcacc
     9901 gaccgagagc ggaaataaag cccgccgccg tattcgtcca tatccttgct ggcgggatgg
     9961 atcgaggtca acggacgctc cttgtattca ctgccatccg tgcgatacgg atggccccag
    10021 ctcatctcgg tttccgcgct gttgtagatg gccagcagat ccagactgtt cttctcgtcg
    10081 aacttcaccg tggccttgat cgcatccatg aagatcgtgc gcgacccgtc aaagggtgtg
    10141 ccgtcgcaga tcacacggcc cgcgccgtaa accggaccgt ccggaccgaa gaaatcctgt
    10201 cgcccgatgc gcagatcgag ccacccgtca gccagattgt agagatccag atagaggctg
    10261 tccaccagca cctcgtccgg cgggcgggca ttgcggctct ctctcggctg gaaatactcg
    10321 cggaactcat cggcgacgcg cagatagagt ttgaaatcct cgttcttcac ctgaccccac
    10381 acgcgcggcc ggatccggag gtagttgtcg tttttggccg tcacgccctc gccctgcgca
    10441 ttgatccgct tcggcaggtt gtcactggcc tcctgccgca cacgcagatc cgcacccgcg
    10501 tcccaatcga gtccgccggt ctcgcgcgcc ttcatctgtg ccacgtccgc aggcgccgcc
    10561 gcgcccgcac cctgtcccgc aggactcttc tcctccgcaa acaggacacc cgccaatccg
    10621 ataccgatga accatcgcaa catgcttttc atatggatcc cttgtttggg gctgttgtgg
    10681 ttttgaaccg cccttccggg cgttacttca agtagtaacg ctttataccg gaaaccgcgt
    10741 atccgtgtcc agcgggattt gcccgtttta ttcagaggtc tgtttttttt aaccgtgtct
    10801 gcccgcttcg cccagatttt gctacactgc cgccatgaca accgtaaccg tatggatcgt
    10861 cggcggcgtg gccgcttacc tgttgggatc gatccccttc ggattcctgc tggcccggag
    10921 ccgcggaaag gatatccgca cgctgggcag cggcaacatc ggggccacca atgtgttccg
    10981 ttcagtcagc aagccgctgg gcgttctgac cttcgcgctc gattttttga aaggcctcgg
    11041 cggcgtcacg ctgattccgg ccctggcctt gacaatctgt ccgccggaaa cgggcgcggc
    11101 agggggcaag gggctggcga tgttctgcgg cgcgctgacg gtcgtcggcc acaattggac
    11161 ctgtttcctc cgctttaagg ggggcaaggg cgtggccacg agcacgggtc tggtgctcgg
    11221 cctcgcgccc caggcggtgg gcatcgcttt cgttgtctgg ctggttgtct tcctcatgac
    11281 gcggatggtc tcggcagcct ctgtggcagc ggccgtgacg ctggccgcgg ccgtgtggtg
    11341 gccactgcgc ctccacgcgg cgtacgggtt ctggttcccg gccgtgttca ccgcccttgc
    11401 ggcggtggcg gtctggaaac accggtccaa cctcgcacgg atacgcaacg gaacagaagc
    11461 gcgtttcacc tttcgtacac ggcggcaggt ttgagcgcag actctgggag agatacggat
    11521 atgcacatca ccatcatcgg cgacggcggc tggggtacgg ccgtcggcct tctactcaac
    11581 ggctatggcc acgccgtcac cctctggggc ccctttccgg aagttgtgga tgaaatccgt
    11641 gaacgccggg agaacacgcg ctttctgccc gggattccgc tccccgaaac cctgcgctgg
    11701 accgccgacg cggccgaagc cgtggcaggt gcggacgtgg tgatcctggc ctcgccctcc
    11761 aaattcctcg cccaggtttg tgaacggttc aaaggcctga tccccgccga ctgtctgacc
    11821 gtcagtctga ccaaaggctt gtgcgagacc acccactgcc ggatgagcca gctcgcccgc
    11881 acgattctcg ggctccgctc gatcgtggtg ctctcgggac cgagtcacgc cgaggaagtg
    11941 gcgcgccggc tccccactgc ggtggtcgcg gcctctgagg aagaggaact cacccgccgc
    12001 gtgcagcagc tcttcagcgg cccggctttc cgcgtctaca cgtccccgga ccccttgggc
    12061 gtggaaatcg gcggcgcggt gaagaacgtc atcgccatcg cggtcggcgt gtccgacgga
    12121 ctcggattcg gcgacaactc ccgcgccgcg ttgatcacac ggggcctggc ggagatcacc
    12181 cgtttcggca ccgcgctcgg cgcgtgtccc gccaccttct cgggactgag cggcgcgggc
    12241 gacctgatcg tcacctgcac cagccgccac agccgcaatc acatggtggg cgaacgactc
    12301 ggccgcggcg agcggatcca agagattctg gacagcatga agatggtggc cgagggcgtc
    12361 tggaacgcca aaatcatcca cgcgctcagc cgtacccacg gcgtttccat gccgatcacc
    12421 gaggttgtgc acgcactctg ttatgagggg ctttcggcgc gggatgccgt cgaggcgctc
    12481 atgacccgcg aggtgaagcc cgagcagtaa agcgcgagat aagccgactg tccggggtgc
    12541 cgggcgaaac cggggggata acgggaacgt tggacgtcgt cctattgcac tttttgtttt
    12601 cattgttaaa atgcgcccat gaaacggaaa cgcgtgctgc acatcgccct gatctacaac
    12661 acctaccggg acgccgttcc cgaggcgccg gatgatcgcg gcagcacgaa tgatttgagg
    12721 cgtttcatcc gcagtctggc gcgcgcgctg cgtagcctcg ggcaccgcgt gaccgtgttg
    12781 ccgctggcca acgacatgct ggggttccag cgtaagctgg cgcgcctgca accggacgtg
    12841 gtcttcaacc aatacgacga tgttgtgcac agcgcgcttt atgagatgcg cgtggccgcg
    12901 ctggtgcgta tgatgggttt cccgatcacc ggctcgcacg ccttggcgct gggcctctgc
    12961 cgctacaagc acatggcggc cagtctgctg caaggcgccg gtctgccggt gccgcctgac
    13021 gcgcggctgc tggagcgcgt gaaagacgtg tccggtgtga agtggcgttt ccccctgatc
    13081 gtccaggccg ccaaagagca tgcgggcgtc ggactgacgc gccactccgt ggtgcactcg
    13141 gtcaccgccc tgcgccgcca ggtcggcatc attctgcgcg actaccatca accagcgacc
    13201 gtgcaacgct tcctgccggg gcgcgagttc aacatcggca tcatcggcgg agccaaacag
    13261 cgtgtgctgc cctttgccga agtcaactac gccgggttgc cccccggcat tcccccgatc
    13321 atgtcctatg acgccaagtg gacggagagc agtatcgaat acaagtgcac gtcggtgatc
    13381 tgcccggcgg acgtcgcgcc gccgctggca cgcaaactca ccaacctgac ggtcaaagcg
    13441 ttccgcgcct tgggcggctg gggctatggc cgcgtggaca tccgcctcga cgaggcgggc
    13501 gagccgcatg tgctggaggt caactgcaac ccctgtctgg aggaggacgt cgcgctggcc
    13561 cgttcggcca aagctgcggg gatcgcctac ccgcagctcc tgcagcagat cctcgacgcc
    13621 gcgttcgaaa agccgccctt cgacaccgat gtcccgatgc tgccgccgcc acgcccgcag
    13681 aatccgctga ggccgacctg actctgtacg tcaggctgac gaaagaaaac gccgaaacgt
    13741 tatcgcgtca acgccactgt gtgccgtcga acgttttcaa tacgccgccc actttgatgg
    13801 agtcgcctgc ctccggcgtc aggccctcca gcgagtggat cgacccgatt ccgttgacgc
    13861 gcgcacggac gcccgcaccc gccgacaccc gcagatcacg ctcctgcatc aggccctcga
    13921 ccgtcaggtt tagtgaagag cccgcccctc ccacagagat gtccgcccct ttcccgtcat
    13981 cggctttcaa caccacgccg gagagatgca gccagacgtc gatcgcgcag tcccggttgt
    14041 cgttcggacg cgcaaggttc acgagcggtc cccgtgtttt ggtgatgttc cgtagccaca
    14101 agcgcacgga ctcgtaaccg ctgaccagcg cacccggcga ttcggagtaa acatcctcca
    14161 gccggacttc acagaccgga tagttctccg gcttcacctc cggatgaatc ccgcggttcc
    14221 atgacgagcg ggtttcccag tccgcgagga aaccgttcct cggcgagcgc aggtaaaggt
    14281 tgcggaacgt gacattgcgg atgttcgcgg cgagcgcgtc gctatcctgc atgaaccaga
    14341 atttcagccc ggtcgcgtcc gtccagatgc cgcgcgtgtg cgtcggcgcc tccagcgaga
    14401 cgtactcgtt cgtgccccgc ggcatcgcca cgcggtagag cttctttccg caccgcacgg
    14461 tgtcgccgcg ctgtagccgg atccccttgt gccagtccgt ccacgcgccg gtcagaatgc
    14521 ggctaaagtt gcaacggccg cggccgtcat ccgtgatgtt ctcgatcaag ccgtcctcga
    14581 tgtcccccac caccggcgcg gaggaaaccc agtccatggc ggtgagcgcg atcccgtcat
    14641 cgccggtctc cagaatccca ttgcggatca cgaattttcg ccccgcattg acatggacgc
    14701 cgtctttcgc gccgcggatc tcgaattcat ccaagatcac attctcgaag gtggcgatct
    14761 gaaacccgta ctggccgcca tgcaggttga ggcaagtgaa cttgcgcacc gtgaggtcgc
    14821 ggatgcggca gaaggagatc tgtccgcggt tgccgtattg cggactgtcc tcgccatgac
    14881 cggcgctgaa gtcattgacg tcgatcgtcg cgccctccac gacgatgttc gtgtcccagg
    14941 cccacgactc gagcccacgg ttggccagca catggacata tgccgcctgc ttctgcagca
    15001 ccgcgccggg cgtgaaaatc agccgcgtat cgctgtccag ccataccgtc cgatccaagc
    15061 cgtagacacc gggccgcgtc acctgcacgg tccgcttgcc gccatccagc gcccgttgca
    15121 gcgccacggc gttcgtcgcc gcaggcgcat ccggcgagaa cccgtagtcc gccggatcca
    15181 caatcgcacc gcgaacacat aacacccatg cgctcaacgc gatggccccc catcctcggc
    15241 tggtcttcca tactgttttc attcaacatt cgcccccgtt acccggacaa agcaatccct
    15301 tacctcatgg ccgccgctca ctggatgctg aacaccaacc cgggcggcac gatcttgatg
    15361 gccaccatag tcgagttcgt gactgcctgg gcccagcggg cttcggtgaa gtcgaaccgg
    15421 atatcccccg tgtacgtgag gttgccggag gccgcgtgaa tgagcggaat gaggcccttg
    15481 tgctctttcg cgaaacgctg cgggtaacgc acccggatcg tgccgtcacc cagaatgtgc
    15541 gtatggctgg tactggtgag gcggatcggc gctgtttcgg cggcgaacac accgtccgtc
    15601 atgtcgaacg tcatgtcgaa tgtattggtg atgaccattt cgtttcggat gcggagatcg
    15661 gactgcggcc ctgccaaccg caacacgctg ttcgagccga cgctacctgt cagtcttctg
    15721 atgtcgacgg tgccgtcacg cacatcgagg attgcggtaa cgcttttcat caccggattc
    15781 gtatgccagc cgccatcgct ggagttcatc tgaatacccg cgttggagtg gaatttcccc
    15841 ccgtctgaca cataaagccc gttggtgccg ccgcctcgat tgattaccag atctgtattg
    15901 caattcgtca ccaaaccacc gctgtcaatc accagccggt taccgtcgtg cttgccgctg
    15961 agctgctctc ctcccagata aaacatggtg cacggccagt tgcagaccgc cccgtcggaa
    16021 acccgcaggg tgcttctagc actgttatat ccgatgcaga tccggtacca gtcgccatgg
    16081 agatcgaacc gggtattcgg ccctttgaac caaaggcaat atccgctgcc gatggtgcgg
    16141 atcatcatag aggagatgtt cccgttggta atggcgaacg tgccgacgca tccgtagtcc
    16201 tgattgagga agttgaccac atacagcgtg tgcccgccga ggttgaggta gttggtttga
    16261 ttcccggcgc tggcgttgag ctgcatcatg ttcaacacat tgatatccga ctccagataa
    16321 agctcttggg tgtcgttggt gaacccgtct ttgaaccaga cccggtcggt ggcgggatcc
    16381 ttcggatacg ttcccatgag cacactgccg ccagcattcg tcacccagtg gttgacgtca
    16441 ttccaactgc caccggtttt agaacgccgg taaaactcga tgttggccgc gcgcgcgtcc
    16501 aatcccacca ccactgccgc gacaaccgtg aggcagagag aaaaacactc ttttttcata
    16561 acaggttcct ttttttgttt gaactccttt gaccgacctt agcggatcac gacactcaga
    16621 ccctgctcga tcacctccag ataaagaccg tctgccttaa ccctcagctt gaggaccttg
    16681 cggcccgtat agccatcgac ggtccattca ctgcccggga tcaaccccga cgacgcaatc
    16741 aacttgtacg accccgtcgt aaacttggac gtgtcacccg tcaggcgcac cgcaccgcct
    16801 gtccagttcg cggtatcggt ccacagtgtc gggttcgccg gcgagccgcc ggccccctcc
    16861 cgatagttcc acgtcgcgct acccgcaaag gtggcctgca ccagagcaag acagccgcac
    16921 gcaacagcac agcgattcat caaccagttt cctttcatgt gtttcattct gacccctttc
    16981 cacactcacg cgaaacattc gcgacccgcc agccagtagc aagagcaacg ctcttgctac
    17041 tggctgacgg gaggttaaat gatcgatccc gatagcgata gcgaccccga ccccgatttg
    17101 gatgaacccc agaacccctg aaaccctgaa accctgaaac catgaaaccc aagaaagtgt
    17161 ctcgtggtta aacagtcgat cccgatagcg atagcgaccc cgaccccgat ttggatgaaa
    17221 ccctgaaacc atgaaaccat gaaaccagaa accatgaaac catgaaacca tgaaacctga
    17281 acccttgaaa cccaagaaag tgtctcgtgg ttaaacagtc gatcccgatc ccgatagcga
    17341 tcccgatccc gatttggatg aaaccctgaa accagaaacc ttgaaaccag aaaccctgaa
    17401 accttgaaac ccagaaacca tgaaacctga acccttgaaa ccaaagagag ttgaaggttc
    17461 aactgctctt tttaggttta ttctaccgcc atcccaaagg gtagaccgat gacacctgtt
    17521 cttatgactg acatttatta tgcagggagg gggcgcaaag agtcaacccc ttgtctctga
    17581 agtttgcgtg ggctctttcc accggttcag ctctttttgg attttgccga agtcgaaatt
    17641 cggtggaacc ggtttatcct gattccgttt gagttcatca agcgtcggct ggggcgactc
    17701 gatgccgttt ttaggaatct ccgcgcccgc gcaccaggcg agaccgttca acagcacctt
    17761 gcggaactgg gggtgaccca gataccagtg gttatgcagg ccggtgaacc cgaacccgcg
    17821 cgtgccgtcc ggacattccg atgcccaggc aacgtgttcg atcgcgcctt tccgcgaacg
    17881 cacaacgggg ttgccgctgt gcggcccgtc cttaccctcg cgcacctcgt caggcggcac
    17941 cgcgctcaga agcggcgtga tgcccgccgt ttccggacgg aaccgcatgt ggaagtacca
    18001 ctcgtcatag aacgcgaagg gggttacgcc gcgtgtgacc ggatgcacgg gcaaggtctt
    18061 gaattccgcc ttccaccatt tgacgaccga ccagtgggcc tcatcgtagc cgccgcacca
    18121 tgccagcgcc cggtcagcca acggcccctt gttgatgatc agggcaaagt ggagcagcgc
    18181 cagtcccact ccgcgcttcc gtaactcatc cagcttctcg agatgcccgt tcaacggatg
    18241 acgcccctcg ccatcactgt tgaccgcgag cgcggtcgcg ccgtcgaaca ccgactcatc
    18301 ctgcggccag ccgtttttgc acacgaccgc ctgaagtccg attccggaag cttccagcca
    18361 cttcttgatc aggacaaaac ccgcgtagtg ttcgtgcgcc atgtatccgt ggctccgttc
    18421 gccggcaatc agcacgagct tcggtgtcgt gcgcgacgcc tgaccagccg caaggcgcgg
    18481 caggaaggcc gtccctgccg ccgcaccggc cgtttttatg aaacccctgc gtgtcgcgtt
    18541 tgtgatcacc tcattcccct ccccctatcc gtcttacagc ctcaccgcct gttcttcaca
    18601 ccggcaactc aaaccccttg cggtaggcct tgcggatcag cggatcggct tcgggcgcat
    18661 ttttcgcccg catcgccgcg ccgtcccact cgatccgctt gttcaggcga atcgccaacg
    18721 tgcccaccag cagcgactcc atgtaagggc ctgaccacca gaacccggcc tgcgcgtctt
    18781 gcggccggcc cgcgatgctg gccgccaccc attcgccgta atgaccgcct ctgttacgcg
    18841 ggatcgtctt cggcggacgc tcgaacgttt tcatggcgct ttcgggcacc agccgtggtg
    18901 cgcccgccca tcctccacac aggatgacgc ccttctcacc cacgaaatag atgccgttgt
    18961 ggtccagctt ccggtccggc tccatgccgg gcggcggagg cggcgtcttg ccgccgtcat
    19021 accacgtcaa tttgatcggc ccgcgcttgg cgtttgcccc aaactcccaa gtgaccacat
    19081 tccacactgg aaacgaatcg gggttctttt cagaggtaag cgcctcgacc gcgtccggcg
    19141 cgccgaggtc cagtgcgtac caggctggat ccgcgttgtg gaccatcatg tcgccgatcg
    19201 cgttgttgcc gtagtcgaac cacggccgcc agacctgggg cacgtaatcg gggtgatacg
    19261 cgtgttcggg cgcgggcccc agccacaggt tccagtcaag cgatgccggc acgggaggag
    19321 tcgccgtggg acggcgttgg ccgttcgagt accagaccct caccgctttg gcacccatat
    19381 tcatgatcgg ccgatcggtc caaacatgga tctccttcac ggcaccgatc acgcccgcgt
    19441 cgagccactc cttggtcaag cgcagcccct cgccggcatg gccttggttt cccatctgtg
    19501 tgacggtctt ggtttcggcc gcgacgtttg ccatcagccg cgcctcctcg atcgtgtgcg
    19561 ccatcggctt ttggcagtac acgtgcttgc caagcttcat ggccaacagc gagatcggcg
    19621 cgtgcgtgtg gtcaggcgtg gcgatggcca ccgcgtccaa ccctttttcc ttctccagca
    19681 tcacgcggta atccttgtgg aacggcaggt cgggatattg tttaaggacg tcggtcgcac
    19741 gcgtcatgtc aacgtcgcat agcgccgcga tgcggacact cgggattgtc gaaaaaatac
    19801 cggcgtggct tttacccatg ccgccgatgc cgatgagacc gagattcagc cgttccgacg
    19861 gtggcggctg acccggcgcg cccagcacat ggcgaggcac aatcctgaac ccgaacgcgg
    19921 cgagcgaact cgccgcaaca aacctccgac gcgacaccgt tgtcatggct ctttaccctt
    19981 tccttattca acacccatta tgcggtgagt tgcaaaagcg tcaacctctg attcggggcc
    20041 tttcgattcg aacagccgtg cgcacggctc actcctcccg gaggggctcg actctcccct
    20101 ggatgatcgc cagcatccgc ggggaaagaa tgtgccgttt ttcatcatcg accagactga
    20161 agcggttgcc gtcgttcggt gtgctgaggt agttccagac acaataggaa ataccgtgtt
    20221 ccttgagcaa caggatcacg tcattcatcc agttctcgcg tgacgtcatg tcgcagtgcc
    20281 ggatggtgcc gaactcgccg caccaaagga tcttgtccgg gtgcttcttc gcgaaatcga
    20341 acgccggctg caaccactcc ctcagaacct tgatgtccat ccgctgatac cggggataag
    20401 gattctttgc gccttcaacg aaccgcttgt aatcgcggta gcgctcgatg tcgccgggat
    20461 agggcattgc ccggttgtag tagagcggcc ccgcctgcaa gaccccgcgc tggtgcgtga
    20521 acccgaacgg atcatagaag tggaacgtgt agatgacatt ctcgtcgtca aagacttgca
    20581 gatccttgag cttgtgacaa ttattccagc atgtgctgcc gatgacaatc ttacgggtcg
    20641 ggttgagttt gcggatctcg ctgatcgtcc gcgccgccag tgtattccac gcctgggggt
    20701 caacgttttt cacttcgttg agcaactcga acacgatgcg gttgtccgcg tgatatcggc
    20761 gctcgaactc cagccagagc gcgacgaagc ggttctgcag tccggcatca tccagcaggg
    20821 ccaccggctc cggcacgtcg cagtagttgc cgaccgcctt gtgcagattc agcaccacgt
    20881 tcagtttgta ctcgcggcac cagccaatga agttatcgat gcaccggaag gtccgttcgc
    20941 ggtagacacc gggcttctcc tccagaacga tctggtcgaa gccgagccgg atatggtcga
    21001 cgccgaacga cgcgatgttc tgcacgtcgt tcttggtgat gtaggtttcg aaatgctcgt
    21061 agtcccccgg cgacagcgtc atcttccact tgtccggcag cacattgaaa cgcttgtagt
    21121 tggtcagcca cccgccgatg cccatgccgc gctcgaagcc gacgaacggc ttggccgcct
    21181 gatgccgctt cgcgtcaacc gcggcaggcg cgttctgcgc aacggcggtg gccaccccca
    21241 acccccagca cgccaacagc agcgcgagag cccgcaagga acgaacataa agacgtgtca
    21301 tcatgaaact cccttgtaat agagtaaagt ccctgtattg aaacacaacg gcacgcgctt
    21361 tgcactctgt ttctgtctcc cctcatggag ccatcgtttc catcatgtcc gcatagtaca
    21421 ggaagaactc cgaaccccac aagccgaggg attcctgctc gcgaagcggg aggaagcgtt
    21481 ggacatcctg tcgggccgtg gaccagtcga tctggccgat gaccacccgc agggtttcga
    21541 tcatccacac ccacgtgaca ttcgtccggc gaccggccca gggtccctgt tggtcaagcg
    21601 catgccccag caaggcaagc ccccttcaat ccctgcaggt aggcgtaaac gcgccgactg
    21661 cggagactct ccagaatctc atccaaggct tcaaacgaga gcgtctgcag atcctcctcc
    21721 tcaatgcgca gcgattcggt caggtagctc atgccgttcc ttttccaagt gatgggcttg
    21781 ttgagataga tcaggtcggc gatggcacgc aagggtgacg caatgaacgc ccaggcgttt
    21841 cgggcaacct ccacggcttc gacgcccgcg cgcggcacca gcgccggaac acgctggaaa
    21901 ctgaacaccc ccagcggggt tacgaactcc cggctacgcg ctaccgtcac gctgctgacc
    21961 cgatagaccg cttcacgcgc cattgccaac atcaataccg gtttgtcagg cggcgcacag
    22021 ttcaactctc tttggtttca tggtttcaag gttttatcca aatcgggatc gggatcgcta
    22081 tcgggatcga ttgtttaacg caaaggcgca gagacgcaaa ggttttgagg gggcgacaag
    22141 catcgcttgc acatactcga ccgcaaccga gcgctaccga tcaataacgg aaaaccatgc
    22201 gtgtctcagg cggcgcttgc aaaacgcctc gtggcatggg cgtcccgccc atgaaacacg
    22261 ggcaagatgc ccgtgccaca cctgaacgca gaacgtcgaa cgcccaacgc cgaagcaaaa
    22321 attcctaaat cctcaatcct aactgctaac tccgcgcata gcgcggaaat tacgctggca
    22381 tccgcccccc tgcggtggta cgctgtaaac ataaggttac aataaattcg cgaaccacgg
    22441 atacgaaatg ccccggatga aaacaaaaac agccgattcg gccgccagcc tcggctttga
    22501 ggccaagctc tggctcgccg ccgacaagct gcgcaacaac atggacgccg ctgagtacaa
    22561 gcacgtcgtc ctgggcctga ttttcctgaa atacatcagc gatgccttcg aggcgatgcg
    22621 gcagaagctg accgaaggca agggcgaata caagggcgcc aaccccgaag atcccgacga
    22681 gtaccgcgcc gagaatgtgt tctgggtgcc gcccgccgcc cgctggtcgt acctccaggg
    22741 cagcgccaag cagccgacca tcggtaggat cgtggacgac gccatgaccg ccat
//
LOCUS       DAOSRB010000020        22325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2512_length_22325_cov_7.401886, whole genome shotgun sequence.
ACCESSION   DAOSRB010000020 DAOSRB010000000
VERSION     DAOSRB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22325
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2512_length_22325_cov_7.401886"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLZ74_02195"
     CDS             <1..371
                     /locus_tag="PLZ74_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOR97163.1"
                     /translation="EGKAERWRRVAAEAARQCGAVWMPTIVSPLPFREALPLVATTLP
                     VFTAALTPAARPLREVFASHSEPPVHAGWFVGPEGDFSEAELAELTATGATPVSLGPL
                     VLRSETACLYGLCALNAAWL"
     gene            431..1786
                     /locus_tag="PLZ74_02200"
     CDS             431..1786
                     /locus_tag="PLZ74_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOR97164.1"
                     /translation="MNQYVRFDSVGGASGDMILAALAALGADLDSIGQTLRSFFPQPL
                     FLHTEPAAAAGLHGLRVAVRCDDSEDHDPHVWPDAHTPAHGPDSGHSHHHRPTPQPGH
                     PHGHTHGHTHGHTHNAGHGAHSHTHRGLREIEQLLASAPISDAARTLTRDVFRALAEA
                     EAAIHGKTPETVHFHEVGAWDSVADIVGACLALEQLGIRGVACGPLPAGTGTIHCAHG
                     EMPNPAPATLELLAGMTVTQTEEPFELVTPTGAALLRVWTRTLEPVPREATVLRSAFG
                     FGSRTLQSRPNVLRATLLEAAAAPAPEPEERLVLETNLDDCNPEWLGALTTELLECGA
                     LDVWQIPATMKKGRAGVILGVLARPADADVLRERIFRGTTTFGIRSHAVRRETLSRRF
                     VTAHTPWGEVPVKLGALRGETLTATPEHDACERLARTHGVTPRQVCEAARQSAGEPKR
                     E"
     gene            1783..2922
                     /locus_tag="PLZ74_02205"
     CDS             1783..2922
                     /locus_tag="PLZ74_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR97165.1"
                     /translation="MKNPESVFKQPPHVVQLLPALDEGGVERGTVELNREFVRHGLRS
                     TVVSNGGRLVERIERDGGRHCRLDLKSKNVLSAPARAGTLRRLLSELRPDLVHIRSRV
                     PGWLFAWANRTLGLPHVSTVHGLNSVNAYSRIMTAGVRVICPSSAVANHIRAHYATPE
                     ERIRVIPRGLDPQQFDPQRLDTAFMAAFRDQFGLAGRFVVLGVGRLTPLKGFDVLLHA
                     TDLARKRMPQIKTVIVGGAETAREGYARELYRLAESLGLDDHVAFAGSQSRMAEIYAC
                     GRVLVSGNHAKPEAFGRTMTEALAMECPVIATRFGGALDIVREGVNGWLVEPGDPAAL
                     ADRLVTASQTELTGLREDALERFSLEAMVRATLAVYAEVLGAESP"
     gene            2956..4230
                     /gene="purD"
                     /locus_tag="PLZ74_02210"
     CDS             2956..4230
                     /gene="purD"
                     /locus_tag="PLZ74_02210"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOR97166.1"
                     /translation="MNILVIGGGGREHALAWRLAQDSCRPKLFCAPGNAGTAAFCTNL
                     PLAAEDLDGLVAWSRENRPDLTVVGPEIALCLGITDALEAEGLRVFGPCRAAARMEGS
                     KCFAKEVMRAAGVPTARSASFRSSAAARAALDDFALPVVIKADGLAAGKGVIIAQTRQ
                     EAEAALVSMLEEAVFGKAGAEVLIEEFLEGEEASILALVDGEHAVLLPSSQDHKRVSD
                     GDQGPNTGGMGAYSPAPVVTPELLPVIRDTVILPVVRELKRRGIVYKGVLYAGLMIGP
                     QGIRVLEFNARFGDPETQAVLPRLGGDLIPALEACINGTLSDSLLTVRPEAAATVILA
                     SGGYPGAYRKGLPVTGLDQAAALDDVLLFHAGTALRDGQIVTAGGRVLAVTALGTGLR
                     AAVGRAYEAVGKIHFEGLHFRTDIAHRALDRE"
     gene            4292..4792
                     /gene="purE"
                     /locus_tag="PLZ74_02215"
     CDS             4292..4792
                     /gene="purE"
                     /locus_tag="PLZ74_02215"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOR97167.1"
                     /translation="MPTPLIGIVMGSDSDWPLVQKAADTLNAFGVAFEARVISAHRTP
                     DLAFEYASSAETRGLKVLIAAAGGAAHLGGVLAAHTALPVVGIPIPGGALNGLDALYA
                     TLQMPAGVPVATVTLGNAGPVNAALLAVQILGTADPELRAKFRAYKEELKRKVLEGDA
                     KINAAL"
     gene            complement(4829..5938)
                     /locus_tag="PLZ74_02220"
     CDS             complement(4829..5938)
                     /locus_tag="PLZ74_02220"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOR97168.1"
                     /translation="MELSLLMQAFDVSGRLVTINPTGNGNVNDTFLAIFRNTFAEEQV
                     ILQRVNPLVFPEPQIIMQNLHRLTEHAHRKLETEGDTTDRVWQMPRIVKTRDGQDYYV
                     DDAGEVWRVITKIASATAFDEAQNAEHAAECGAVLGHFHGLVSDLDPRSMTDPLPGFH
                     ITPGYLAKYDAVLESEPLAKKRLGASTEARRMAMFIEERRGVAATLQRALEAGELTLR
                     IMHGDPKVNNIMIDDFTGKGTAIIDLDTVGPGLIHYDFGDALRSICNHAGEDEQNLSK
                     VVFDLDLCEAFCKGYMAHAGEFLTDTDRYYLYDAIRLITFELGLRFFEDYLAGSVYFK
                     IRHPEQNLNRARVQFRLCESIEARERAIRNLLAAL"
     gene            complement(6002..7021)
                     /locus_tag="PLZ74_02225"
     CDS             complement(6002..7021)
                     /locus_tag="PLZ74_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOR97169.1"
                     /translation="MRTGLWAWTAIVLGIGLSSPLFGDEVLFKSGDRLTGKVEKVADG
                     KMTFKSNVAGPLVLNMEDIKTFSTDEPIELALADGSLQKQKVAASDEGYVTIAGTAQP
                     ASLPIANIAKINPDKPRWKGVVGAGATLVRGNTKSDTASVTAEAARRGENDRISFGAG
                     YYFAKQRDNSTRDDSTIADNWFLKGQYDYFFSKQFYGYGNLRYEKDRIANLDIRLTPG
                     LGVGYQWIEREDFNFSTEAGANWVYEKYTEPDETRTYMAGRLAYHVDKSFNDRVKGFH
                     NLEYIPSFERADTFLVNADVGLRAAITARMAVEAKTQLAYNSKPSEGRDKKDLRHILG
                     VAWTF"
     gene            7272..7868
                     /locus_tag="PLZ74_02230"
     CDS             7272..7868
                     /locus_tag="PLZ74_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97170.1"
                     /translation="MSGGTSHPDAGKRCGAVLMETVLAIPLFMIFLGGVMWVGDLLVT
                     RQQLVIADRFVAWNHGLRYPDKEKTDPGTIHRYFFTEANGAPSPYHQPSSSDARIDET
                     FDWSHAASGQVRLQMRMPQWVRSMFNAGQVFYDSGVPLEQASNLFGRDKEDQRHVVVM
                     RTRREAQADYIRNRYGRDKSAEVAEQWEAISDEAWPYE"
     gene            7846..8223
                     /locus_tag="PLZ74_02235"
     CDS             7846..8223
                     /locus_tag="PLZ74_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97171.1"
                     /translation="MRRGPTNSLACAALGLLGLALAAAGAPQAPLPRLPLPPLATVLR
                     EDLNSRTWRQEGELSGTLENASREFRQALAGGGWRLEKRIVAGRSHLLLCSRKRTRFL
                     VMVWEKGVGACGFAWGEDAQQGP"
     gene            8238..9041
                     /gene="cpaB"
                     /locus_tag="PLZ74_02240"
     CDS             8238..9041
                     /gene="cpaB"
                     /locus_tag="PLZ74_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOR97172.1"
                     /translation="MKNFIPLILAVLLGLAAVLAVGRLLQARKQAAEKMTSVVAVARD
                     IAAGDVLTADSIMEKKIPVSARPAQAIFWSRSEMVKGQKALRSIAQGDYVLLSDVGLS
                     RSMADIVGEGEWAVALTLPPGGIARLVQPGDEVAVIGTFRIQSKVVSADLSAAPAEVA
                     KEATLVLFPRVRVLDVATDRAGGDEAAGELILALPPQQAQVLVAAQREAQLTLALRRP
                     GDTAAINRMDIGMVDAETFDKLLKGLKPVEMPTIPGQIDAAGSGKPAGQ"
     gene            9060..10634
                     /locus_tag="PLZ74_02245"
     CDS             9060..10634
                     /locus_tag="PLZ74_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOR97173.1"
                     /translation="MSTFRNRMRVITSAMASAVFALVMSPALARAADAEPVTVTVGKM
                     EVINVAFPIVGFRMADASVAKVEQQGQQQLRVMGLRAGTTDLQVAGEGNVSAIFTLTV
                     TDNINAVLAAVKRDLDTVPEVDVAINLGRVLIKGEVSNIEHWNYLQKVVALYKDNVVN
                     LASFRPAPEVMLGLKAAFEKAGVKVLGTEAPAESAEPGSVKLAFSGNNIFVSGQVYSQ
                     RDKDRILQIIEAQNWITFKRGGDEKQDATKPVNEGAVRALVDLTIVPTMIEMEAVFVG
                     VTDAEEKQIGVNLAKAGLLVLDSTSAGFQGDLATRSSGWAGTYTINSGLQGALKFFAG
                     NGPGRFQNAGHMTFKNDSPEWRTYHSGGTLKVRVATRDATSLEDVDYGLLMKVKGGLL
                     NAKAAALDVNLELSYPVPVGNDYDLKRNRIETTVNCPIGQTIVLAGMKSLVEQSNMEG
                     VPFLRSVPAISWFFSEKNTRKEDSKVLILMSPRIAGMPIETPAVSEQTAPALQESDKP
                     VQQRQLEKQKRRFFFF"
     gene            10641..12320
                     /locus_tag="PLZ74_02250"
     CDS             10641..12320
                     /locus_tag="PLZ74_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOR97174.1"
                     /translation="MATLTLIPSGGSGEAEVYTLEPAGVTTLGRSPENRIVLHDNRVS
                     RAHLTVTGKGGVFRVRDLDSTYGTLLNGKRLTTEATLREGDSLQIGGFSLRFGQGTKE
                     TPSETPAADAQHAAEPDIEMDPALAAMAALYTEEMMTMKRRIHEQVLFKLNLPEIATK
                     QIQDEEMRAKLENALDQVLREVRHELPRDLINNDLFRQALLDELIGYGPITPMLRDPT
                     VDEVMVNGPARIFVERKGVLYETGMRFFDDRHLITIIQRIVEPLGRHVDEASPMVDAR
                     LPDGSRVNAIIPPLALDGSSVTVRKFAKRKLTTDDLIAFGSMTPEIALFLEEAVRARQ
                     NIVVSGGTGSGKTTLLNVLSQFIPRNERLVTIEDSAELKLSHRNIVRLEARPANIEGR
                     GRVAIRDLVINALRMRPDRIVVGECRGAEALDMLQAMNTGHDGSLTTAHANTPRDCLA
                     RLENMVMMAGFELPSAAIREQIASAINLIIQQNRLADGSRKIVTISEVTGREGNTILL
                     QDIFVFEQKGFSPEGKVLGQMVATGNIPQFVEALKQRGDLRLDMGVFVPKA"
     gene            12352..13233
                     /locus_tag="PLZ74_02255"
     CDS             12352..13233
                     /locus_tag="PLZ74_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOR97175.1"
                     /translation="MTDILVLVLVFAATTWGVWVVTPLLAQIGLERSATQHIRSDGVK
                     QLGPIYRFTTPERLAQACWSAALLGAGLAAAALIAFNVLSPMLIGGACLLAGVIAFHI
                     PAKWVQHRIRTRQRLFDARLTDLTLGLANGLRAGAALPQSLEIVSRDMGGPMTEELML
                     VLQEYRLGMDLPEALSRLCERMPGEDLSLLVTAIRLTMQSGGSLAEVLDRITDTIRQR
                     TEFKDRLLTMTAQGRFEAIAMAAAPLVAFLILFFLDRELMQPLLQTKIGWCAIGAVVI
                     LETVGFLVINKIVTIDV"
     gene            13245..14144
                     /locus_tag="PLZ74_02260"
     CDS             13245..14144
                     /locus_tag="PLZ74_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOR97176.1"
                     /translation="MDVIIIQMLTFLSVLFLVNGIQAVIRKENETLSNAKPLVFRVFH
                     HEIGTIGRATGAWIDQTFPVQTRQIRNDLIAGAMPIESVEVRGLQGFAGAVLFAVAGL
                     TTFILTLNWAYGLLAGIGFGLMGWYYPVAWVSRCARTRKDLMSKSMPYAIDLITVSME
                     AGQDFGAAVRNLVHDGPKGPLRDEFGVTLRQIELGKSRVEGLKAMADRVQLDEFRSLV
                     TSVVQSSEMGASISATLKIQAEEIRRRRYQRAERQAARAPSLMLIPTALFILPAVFII
                     IATPIIIRAIESGVGEYFQSGGG"
     gene            14150..15823
                     /locus_tag="PLZ74_02265"
     CDS             14150..15823
                     /locus_tag="PLZ74_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOR97177.1"
                     /translation="MFCLKVEKGEPAGEVHILKEGENTLGRSRSATFRLGASDVSGQH
                     ARIVVRGGVVTVENLSQFGTKVDGTPITGSVQLTEGQRLSMGESTVLCLLRTPAGEPS
                     AGADMSAAEPDVTQTKGTLGVEAILQARADEIPTGEGVPPSPETGGATRATPPPPPPP
                     AQVAAAAPTGAEFGAPVPAYPDSDLTSGLPLEALTVDSETIEGATRAMQTRAATPEEI
                     EHLRIVEQKRHKKRITRALAVLIPLIALVVIFRPRTPPPEPKIEWAKDEEGNYLDLFE
                     AAPSGGLAEGGYDLCYPGPVRFDKTVTEDGVILSGFVGRERNVPMRLITQEQTDPKFV
                     TMDRLAFVEEWIQQQKADNKRWSFDKPSPMPGFIGIRNGIPYLRVTYYRDDDEGSWFG
                     VASVFRYGYRRIVIRAEAPASERVRLEDLLSSKFMRASEAMEYAYWEAPEELPKQSEA
                     VLLKQAEADLNRMAPATWFELQRNLSGLLARTVLSGNKTTEVEALRLLVRLRERLTLW
                     FNSQQLAFDAARMQNNRGQARETARLTRAVFKDMEDQRYYTVRKWNTEF"
     gene            15828..18125
                     /locus_tag="PLZ74_02270"
     CDS             15828..18125
                     /locus_tag="PLZ74_02270"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOR97178.1"
                     /translation="MLIRLEHNGRIIHEIGSDALTAELTIGRSHACEWPIPKEDGVAS
                     SRHAALFKKGRAVWVKDLKSTNGTFHNGKKIEKRKLAIGDKISIGNCVLSAEADRSGT
                     GRNLSEVVVLSGKTRGQKKRLVPPVFTIGSDPSSSLVFLDMLVSRRHAEILIREDGSC
                     WIRDLGSKNGTSVNGMPLRDDKERLLKDGDCIACSHFELEFRDGAVKHSNKQTWLRLG
                     ILIVTLLAGLGAYHTYQRLKPSAEAFVKAARRLAAKEEFDQAEREVEQAVNARHASRH
                     QVAIEEMRRLIGVWKRTRALWQSAKEDLERGEWVGASRTLGVLQASKREVWEWNAQAA
                     EAKDAAAYAKTLLDAFLQTDALLKREDLEFAELENNQPVLDRVLDEKPATAEPAAYLK
                     RLLDELRRVQTRQTALLTESRQMETALTKLGEAQPSYDEVVSLLEQLATSQEPALRRR
                     AQVLVVPVKALANSYIKLGEAATTLRSLAFQSALKLDLALPTVNACAIEPRVSLARQN
                     IEKIHANLKLKGYQLDSLFTEVEKRIGRNDDSLEGFRVLRNSAMMEKVMACDSMNLPL
                     PKRSRSQPAGEYDRLLGIEAFYAYLSAQPEPLDPAMLADLPFVPLINQTREALQKIDT
                     FLQFMAQADNQWLLDDALAVQVKRMKAIMQQRDELVKLLITRAGSATDRTALIAGGLA
                     ARLITVSGKTKIKDLPPEEWLAAELKRQRTALLRMNEEYTLAAPTRQIEIRAAVLKAG
                     LPGDPVVRRMWAMRDAAAAAMSSTP"
     gene            complement(18170..18784)
                     /locus_tag="PLZ74_02275"
     CDS             complement(18170..18784)
                     /locus_tag="PLZ74_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOR97179.1"
                     /translation="MDVSAKRMIVTAITFGRVPFVLLFMVLAVTHAYMAPRGVWLAVA
                     ATASLSIAALTDLCDGKLARKWQVVSRFGAMADPLMDKVFYLVVFPTLLWLLGRQPNE
                     AEHALVMLIFTVLYILRDQWVTFLRSVGAMFQADCRANWMGKFRTAVSFPAACVIYVY
                     ASLHPWFLPQALIYLIEALLIILNLWSIVVYTRQYLPYIKQALQ"
     gene            complement(18805..20244)
                     /gene="uxaC"
                     /locus_tag="PLZ74_02280"
     CDS             complement(18805..20244)
                     /gene="uxaC"
                     /locus_tag="PLZ74_02280"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOR97180.1"
                     /translation="MKPFIHDDFLLDNAAARELYHGYAATMPILDYHCHLPPAEIAED
                     RRWENMTRLWLGGDHYKWRALRSNGVDERFITGDAPDREKFQKYAETLPYLLRNPLFD
                     WSQLELARYFGITDLLTPATAEAIWTETGRQLAEPAFSARGLMVRSNVRLVCTTDDPA
                     DSLEHHAALRAENFAVRVLPTWRPDKALALDRPAFWNAWLDRLSAAADMPVKTWDDLL
                     CALQRRHEVFAAHGCRLSDYGLDTVYADPYLDTEIREIFHRVRQGGKATPEEVSAFRS
                     AFLYESLTLDAKSDWSAQIHYGAMRNNNTIMFKRLGPDTGFDSIGDWHVASPMARLFD
                     RLEQAGALPRTVIYTLNPRDNEMVATLLGNFQRGPEPGRMQFGSGWWFNDQRDGMLRQ
                     MEALSQLGLLSRFVGMLTDSRSFLSYTRHEYFRRVLCNLLGTDITQGRIPNDLAWTGE
                     IVKDICTRNAERYFGFDLPDAPVLSFQER"
     gene            20554..21108
                     /locus_tag="PLZ74_02285"
     CDS             20554..21108
                     /locus_tag="PLZ74_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR97181.1"
                     /translation="MAQLSITFCRKLARARRERGMTQSALAQAVSCAQSAISMLESGQ
                     PGKLSQETVGKIASLLGVPLEDSAPDGVPLPTRPSAGYCPNAACFSNIPYVVADEVLF
                     WPRPQPTAARGPYCVYCGEVLEPQCPHCGAGVTPGACCPQCGRARITGGAPDGVDPED
                     WAARRRRELADWRALSAWTGFASE"
     gene            21152..22093
                     /locus_tag="PLZ74_02290"
     CDS             21152..22093
                     /locus_tag="PLZ74_02290"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOR97182.1"
                     /translation="MTKKLLIVTDGKAGHENQSKAFCAALGYGYECVRAVYPSRFHKG
                     LSYLIDWLGLLLSSPFTIEPTDGYFAAVVCTGSTAFYPGKIEARRRGIPVAAILYPSG
                     YRKANFDCILAPSFDRPRPAPNVIPIPVNLTSTDAAFYETGVAAFRARHTQRKPASAL
                     IVGGPNAAAELSVETLRRELDRFFAVTEGNERWVTTSRRTPPAIEEFIASRPFDYSLL
                     YSRDTFNPIPAFVSLCEHLFVTADSTGMISEAVTRGRARVEILMTLRKPDSKFGRFIR
                     DLEQAGAVHVFDGTPGCADRKIDLAPAFEQARKLLGL"
BASE COUNT         4201 a         6854 c         7227 g         4043 t
ORIGIN      
        1 gggaagggaa ggcggaacgg tggcgacggg tggcagcgga ggcggctcgc cagtgcgggg
       61 cggtctggat gccgaccatt gtgtcgcccc tccccttccg cgaggcccta ccccttgtcg
      121 caaccaccct cccagtcttt acggcggcgc tcacgcccgc ggcccgcccg ctgcgcgagg
      181 ttttcgcgtc ccatagcgaa ccgcctgtcc acgccggatg gtttgtcgga cccgagggag
      241 atttttcgga ggccgaactg gcggagttga ccgcaacagg cgcgacgcca gtgagcctcg
      301 gtccgttggt gctgcggagt gaaaccgcct gtctctatgg attgtgcgca ctcaacgccg
      361 cctggctgta aaaaaacgcc ggggcgcgtt ttgcgttgag ccgtatcgca ataccttgta
      421 ttctgcacac atgaaccaat acgtgcgctt cgacagtgtg ggcggggcca gcggcgacat
      481 gatcctcgcc gcgcttgccg cgttgggggc ggatctggat tctatcggcc agacgctccg
      541 ttcgtttttt ccgcaacccc tgtttctgca taccgaaccc gcggctgccg cgggactaca
      601 cggactgcgg gtagcggtgc ggtgcgacga ttccgaagat cacgacccgc acgtctggcc
      661 ggatgcgcac acccccgcgc acggtcctga ctccgggcac agccaccacc acaggccgac
      721 tccccagccg ggacatccac acggacacac gcatggacac acgcacggac acacacacaa
      781 cgcggggcac ggggcgcaca gccacacgca ccgcggcctg cgcgaaatcg aacagcttct
      841 ggcctccgcg ccgatctccg acgccgcgcg gaccctaacc cgcgacgtgt tccgcgctct
      901 ggccgaggcg gaagccgcga tccacggcaa aacgccggaa accgtccatt tccacgaggt
      961 cggcgcctgg gattcggtcg ccgacatcgt cggggcctgt ctcgcgctcg aacagctcgg
     1021 aatccgcggt gtggcctgcg gccctctgcc cgcaggaacc ggcacgatcc attgcgcgca
     1081 cggtgaaatg cccaatcccg ccccggccac gctggagctg ttggccggca tgaccgtgac
     1141 gcagaccgaa gagccgttcg agttggtgac ccccaccggc gcggcgctgt tgcgcgtctg
     1201 gacgcgtacg cttgaaccgg tgccgcgtga ggcgaccgtc ctgcgcagtg cgttcggctt
     1261 cggcagccgt acgctgcaga gccgcccgaa tgtgctgcgc gccacgctgt tggaggctgc
     1321 cgccgcgcct gcccccgaac cggaggagcg gctggtgctc gaaaccaatc tggacgactg
     1381 caaccccgaa tggctgggcg cactgacgac cgaactgctg gagtgcgggg cactggatgt
     1441 ctggcagatc cccgcgacca tgaagaaagg gcgagcgggc gtgattctcg gcgtactcgc
     1501 gcgaccggcc gacgcggacg tgttgcggga acgcatcttc cgcggcacga ccacctttgg
     1561 catccgctca catgcggtgc ggcgcgaaac cttgtcgcgg cgcttcgtga ccgcgcatac
     1621 gccgtggggc gaggtaccgg tcaagttggg tgcgttgcgt ggcgagaccc tcacggccac
     1681 accggagcat gacgcttgcg aacggttggc ccgtacccac ggcgtgacgc cgcgccaagt
     1741 atgcgaggcc gcgcgccaat cagccggaga accgaaacgc gaatgaagaa tccggaaagc
     1801 gtgttcaaac agccgccgca tgtggtccaa ctgctgccgg cgcttgacga gggcggcgtg
     1861 gaacgcggca ccgtggagtt gaaccgcgaa tttgtgcggc atggcctgcg cagcaccgtg
     1921 gtttcaaatg gcggacggct tgtcgaacgc atcgaacgcg acggcggtcg gcactgccgc
     1981 ttggacctca aatccaaaaa cgtgctcagt gccccggcgc gtgcgggcac gttgcgccgc
     2041 ctgttgtccg aactccgccc cgatctggtg catatccgca gccgcgtgcc cggatggctc
     2101 ttcgcctggg cgaaccggac actcggcctg ccgcacgtct caaccgtcca cgggctcaac
     2161 agcgtcaatg cctacagtcg gatcatgacc gccggggtac gggtaatctg tcccagttcg
     2221 gctgtggcca accacatccg tgcgcactat gccacgccgg aggagcgcat ccgcgtgatc
     2281 ccgcgcggtc tggatccgca acagtttgat ccgcagcggc tggacaccgc gttcatggcg
     2341 gcgtttcgtg accaattcgg tctggcgggg cgtttcgtgg tgttgggggt gggacggctt
     2401 acgccgctca aggggtttga cgtgttgctg catgcgaccg atctggcccg caagcggatg
     2461 ccccaaatca aaaccgtgat cgtcggcggg gccgaaacag cgcgcgaagg gtacgcgcgg
     2521 gagctgtacc gccttgcgga gtcgctgggt ttagacgacc atgtggcctt tgcaggcagc
     2581 cagagccgca tggcggaaat ctacgcctgc ggccgggtat tggtttccgg caatcatgcc
     2641 aaacccgagg ccttcggccg caccatgacc gaggcgctgg ctatggagtg tccggtgatc
     2701 gcgacacgct tcggcggcgc gctcgatatc gtgcgcgaag gggtgaacgg ctggctggtg
     2761 gagcccggag atccggcggc gctggccgac cggctcgtga ccgcttcgca aaccgagttg
     2821 accgggctgc gcgaggacgc gttggagcgt ttctctctgg aggcgatggt ccgcgccacc
     2881 ctcgccgtgt atgcagaggt gctgggcgct gaatctcctt gaatccggcg gggggcttcc
     2941 cgtatcttat cgcgtatgaa catccttgtg atcggcggcg gcggccggga gcacgccctc
     3001 gcttggcgtt tggcccaaga ctcctgtcgt cccaagttgt tttgcgcgcc cggtaatgcc
     3061 ggaaccgccg cgttctgcac caacctgccg ttggctgcgg aggatctgga cggactggtc
     3121 gcctggtccc gcgagaaccg tccggatctc acagtcgtgg gcccggagat cgcgctctgc
     3181 ctcggtatca ccgacgcgct ggaagccgaa gggttgcgcg ttttcggccc ctgccgggcc
     3241 gcagcccgca tggagggaag caaatgtttc gcgaaagagg tgatgcgcgc ggcaggtgtc
     3301 cccacggccc gttccgcgtc tttccgttcg agtgcggcgg cacgggccgc gctggatgat
     3361 ttcgcgttgc cggtggtgat caaggcggat ggacttgcgg cgggcaaagg ggtgatcatc
     3421 gcgcagacgc ggcaagaggc ggaagcggct ctggtttcga tgctggagga ggcggtcttt
     3481 ggcaaagccg gcgccgaagt gttgatcgag gagtttctcg aaggggaaga ggcctcgatc
     3541 ctggcgttgg tcgatggcga acatgcggtg ctgcttccct cctcgcagga tcacaaacgg
     3601 gtgtcggatg gcgatcaggg accgaacacc gggggcatgg gggcctactc gcccgcgccg
     3661 gtggtcacgc cggagttgtt gccggtgatc cgcgacacgg tgatcctgcc tgtggtgcgt
     3721 gaattgaagc ggcgcggcat tgtttacaaa ggtgtgttgt acgcggggct gatgatcggt
     3781 ccccagggca tccgtgtgct ggagtttaac gcgcgattcg gcgatccgga aacccaggcg
     3841 gttttgccgc gtctcggcgg cgacttgatc ccggcgctgg aggcctgcat caacggcaca
     3901 ctgagcgatt cgctgctgac ggtccgtccc gaagccgccg ccacggtgat tctggcctcg
     3961 ggcggatatc ccggcgccta tcgcaaaggg ctccccgtga cggggctgga ccaagccgcc
     4021 gcactggatg acgtgctgct ctttcatgcc gggactgctt tgcgggacgg ccagatcgtc
     4081 acggcgggcg gccgcgtgct ggctgtcacc gcgctgggta ccgggttgcg tgccgccgtg
     4141 ggccgcgcct acgaggcggt cgggaaaatc cattttgagg gtttgcattt tcgcaccgac
     4201 atcgcccacc gcgcgttgga ccgggagtga cggacaacac acgatgcttg gaacagattt
     4261 ccgaatgctc taaaccaagg agacctcaga catgcccaca cctctcattg gaatcgtgat
     4321 gggaagcgat tcagactggc ctctcgtgca gaaggccgcg gacaccttga acgccttcgg
     4381 cgttgccttc gaggcgcgcg tgatctccgc gcaccgtacg ccggatctcg cgttcgagta
     4441 tgcgagcagc gcggagacgc gcgggctgaa ggtcctcatc gcggcggcgg gtggcgcggc
     4501 ccacctcggc ggcgtgctgg cggcgcacac ggcgctgccg gtagtcggca ttccgatccc
     4561 cggcggcgcg ctgaacggac tggacgcact ctacgccacg ctccagatgc cagcaggggt
     4621 tccggtggcc acggtgacgc tcggcaacgc gggtccggtc aacgcggccc tgctcgccgt
     4681 gcagatcctc ggcaccgccg acccggaact ccgtgcgaaa ttccgtgcct acaaagaaga
     4741 actgaagcga aaagttctgg aaggcgacgc caagatcaac gcggcgctgt gacgcgtaag
     4801 atttcccggc ctgttgacgg cgcactcctc acaatgcggc cagcagattg cggatcgcgc
     4861 gttcgcgcgc ctcgattgat tcgcagaggc ggaactggac acgggcgcgg ttgaggttct
     4921 gttccgggtg gcggatcttg aaatagacgc tacccgcgag ataatcctcg aagaaacgca
     4981 gacccaactc gaaggtgatc aggcggatcg cgtcgtagag gtaatagcgg tccgtgtcgg
     5041 tcaggaactc tccggcgtgc gccatgtagc ccttacagaa ggcctcgcac aggtcgagat
     5101 cgaagaccac cttgctcaga ttctgctcat cctcgccggc atggttgcag atcgaacgca
     5161 gggcgtcacc gaaatcgtaa tggatcagac cgggacccac ggtgtcgaga tcgattatag
     5221 ccgtgccttt gccggtgaag tcatcgatca tgatgttgtt gaccttgggg tcgccatgca
     5281 tgatgcgcag ggtgagttcg cccgcctcca gcgcccgctg cagcgtcgcg gcgacccccc
     5341 gccgctcctc gatgaacatc gccatgcgcc gcgcctccgt ggaggcgccg agtcgcttct
     5401 tcgccagcgg ctctgactcc agtacggcgt cgtatttggc cagatagccg ggcgtgatat
     5461 ggaaaccggg cagcggatcg gtcatgctgc gcggatcgag atccgagacc agcccgtgga
     5521 agtggcccag caccgcgccg cactcggcgg cgtgttccgc attctgggcc tcatcgaagg
     5581 ccgtggcgga ggcgatcttg gtgatcacgc gccagacctc acccgcgtca tccacataat
     5641 agtcctgccc gtcgcgcgtt ttgacgatgc gcggcatctg ccacacgcgg tcggtggtat
     5701 ccccttccgt ctccagcttc cgatgcgcgt gctcggtcag ccggtggagg ttctgcatga
     5761 tgatctgcgg ttcggggaag accagcgggt tgacgcgctg caggatcacc tgctcttccg
     5821 caaaggtatt gcggaagatg gccagaaagg tgtcgttgac gtttccgttg ccggtgggat
     5881 tgatggtcac cagccttccg gaaacatcaa acgcctgcat cagcaacgac aattccataa
     5941 catgcccccg atttccgtaa cccctgcctg cgcacgcggc gcggcagggg gcccgtcccg
     6001 ttcagaaggt ccacgccaca ccgaggatgt ggcgcaggtc tttcttgtca cgcccttcgg
     6061 agggcttgga gttatacgcc agttgcgtct tggcctcgac cgccatgcgc gcggtgatgg
     6121 cggcgcgcag ccccacatcg gcgttgacca gaaaagtgtc ggcccgctcg aagctgggaa
     6181 tgtattcgag attgtggaac cccttcacac gatcattaaa ggatttgtcc acatggtagg
     6241 ccagacggcc ggccatatag gtgcgggttt cgtccggttc ggtgtacttc tcatacaccc
     6301 agttggcgcc ggcctcggtc gagaaattga aatcctcccg ctcgatccat tgatatccga
     6361 caccgaggcc cggcgtgagc cgtatatcca gattggcgat gcggtccttc tcataccgca
     6421 ggttgccgta gccatagaac tgcttcgaga agaagtagtc gtattggccc ttgaggaacc
     6481 agttatccgc gatggtactg tcgtcgcggg tgctgttgtc ccgctgtttg gcaaaatagt
     6541 acccggcacc gaatgatatg cgatcgttct cgccccggcg cgcagcctcg gccgtcacac
     6601 tggcggtgtc gcttttggtg ttgccgcgca caagggtggc gccggcaccc accacgccct
     6661 tccagcgggg cttgtcgggg ttgatcttgg cgatgttggc gatcggcagc gatgcgggct
     6721 gcgccgttcc ggcaatcgtc acataccctt cgtcggaagc ggccactttc tgtttctgca
     6781 gagagccatc agccaaggcg agttcgatcg gctcgtcggt cgagaaggtt ttgatgtcct
     6841 ccatattcag gacgagcggt cccgccacat tagatttgaa ggtcattttc ccgtccgcca
     6901 ccttctcaac cttgcccgtc aggcggtcgc ccgacttgaa cagcacctcg tcgccaaaaa
     6961 gcggggatga caacccgata cccagcacga tagctgtcca agcccacaaa cctgttctca
     7021 tgacttctcc gtttcctttc ttctgctctc cgcaccccta tgcgcaccaa acaccgggga
     7081 cctgcaagaa tcatttgttt gcaacacctc gcatcgtatc ccaccgtccc agacaaaaca
     7141 agcggtttta tacacgctga aagagtttct ttttatgctt gttcaataaa gcggactttc
     7201 ggtataatgc tcaccgcgac ggtggttggt aatcttgtat catccaaaga cgttagggaa
     7261 acgaggaacg aatgtccggt ggaacctcac atccggatgc cgggaagcgg tgcggtgcgg
     7321 tgctcatgga gacggtgctg gcgataccgt tgttcatgat ttttctgggc ggcgtgatgt
     7381 gggtcggcga tctgctggtc acccggcagc aactggtgat cgccgaccgg tttgtggcgt
     7441 ggaaccacgg gttgcgctat ccggacaagg agaagaccga tccgggcacg atccaccggt
     7501 attttttcac cgaggcgaac ggcgcgccga gtccatatca ccagccgagt tccagcgacg
     7561 cgcggattga tgagacgttt gattggagtc atgcggccag cggccaggtc cggttgcaga
     7621 tgcggatgcc gcagtgggtg cggtcgatgt tcaatgcggg tcaggttttt tacgatagcg
     7681 gcgtgccgct ggaacaggcc agcaacctgt tcggacgcga caaggaggac cagcgccacg
     7741 tggtggtgat gcggacgcgc cgggaggcgc aggcagatta catccggaac cggtacggcc
     7801 gcgacaagtc cgcggaagtc gccgagcaat gggaggcaat cagcgatgag gcgtggccct
     7861 acgaatagtc tcgcgtgcgc ggcgctcggg ttgctcgggt tggcgctggc tgcggcgggt
     7921 gcgccgcagg cacccttgcc gcggctaccg ctcccgccgc tggccacggt cctgcgcgag
     7981 gatctgaaca gccggacttg gcgtcaggag ggcgagctga gcggtacgct cgaaaacgcc
     8041 agccgggagt tccggcaggc gttggcgggc ggcggatgga ggctggagaa gcggattgtg
     8101 gcgggccggt cgcatttgtt attgtgtagc cggaaacgga cgcgtttttt ggtcatggtg
     8161 tgggaaaaag gagtgggcgc ctgcggcttc gcctggggtg aggacgcgca gcaaggcccg
     8221 tgaaaggaaa cggtcggatg aaaaacttca ttccgttgat tctggctgtc ttgttggggt
     8281 tggcggcggt gttggcagtc ggaaggctgc tacaggcgcg caagcaagcg gcggagaaga
     8341 tgacctcggt ggttgcggtc gcgcgggaca tcgcggcagg cgacgtcctg accgccgact
     8401 cgatcatgga gaagaagatc ccggtctcgg ctcggccggc acaggcgatc ttctggtcgc
     8461 ggtcagaaat ggtaaagggc cagaaggcgc tgcggtcgat cgcccaggga gattatgtgc
     8521 tgctttcgga cgtgggcctc tcgcgcagca tggccgatat tgtgggcgaa ggcgaatggg
     8581 cggtggcgct caccctgccg cccggcggga tcgcgcgcct ggtgcaaccc ggcgacgagg
     8641 tggcggtgat cggcactttc cgaatccaga gcaaagtggt ctcggccgac ctttcggccg
     8701 cgcccgctga ggtggccaaa gaggccacgc tggtcctttt cccgcgcgtc cgggtgctgg
     8761 atgtggctac ggaccgcgcg ggcggcgatg aggcggcagg cgagttgatt ctggcgctgc
     8821 cgccgcaaca ggcccaggtg cttgtggccg cgcaacgcga ggcgcaactg accctcgccc
     8881 tgcgccgccc cggcgatacg gcggccatca accgaatgga tatcggaatg gtggatgcgg
     8941 agacattcga caagttgttg aaagggctga aacccgttga gatgccgaca atcccggggc
     9001 aaatcgatgc cgcgggatca ggtaaaccgg cgggacaata atccgaacgg aggaaacaca
     9061 tgagcacatt cagaaacaga atgagagtca taacgtccgc gatggcgtcc gcggtgttcg
     9121 cgctggtgat gtcgcccgct ctggcacggg ccgcggatgc ggaaccggtg acggtgacgg
     9181 tcggcaaaat ggaggtcatt aacgtcgcct tcccgatcgt gggattccgg atggccgatg
     9241 catcggtggc caaagtggag cagcaggggc agcagcagct gcgcgtcatg ggactgcggg
     9301 ccggaacaac cgacctgcag gtggccggcg aagggaacgt ctcggcgatc ttcacgctga
     9361 ccgtgaccga caacatcaac gctgtgctcg ctgcggtcaa acgggacctc gacaccgtgc
     9421 cggaggtgga tgtggcgatc aacctcggcc gcgtgctcat caagggcgag gtcagcaaca
     9481 tcgaacactg gaactacctg cagaaggtgg tggccctgta taaggataac gtggtcaatc
     9541 tggcctcgtt ccggcccgcg cccgaggtga tgctgggact caaggcggcg ttcgaaaaag
     9601 caggcgtgaa ggtgctgggc accgaggccc ctgcggaatc cgccgaaccg ggcagcgtga
     9661 agctggcctt ctccgggaac aacatcttcg tgtccggaca ggtctacagc cagcgcgaca
     9721 aagaccgtat cctgcagatc atcgaggcgc agaactggat cacgttcaaa cggggcggcg
     9781 acgagaaaca agacgcgacc aaaccggtga acgagggggc agtgcgggct ttggtggatc
     9841 tgacgatcgt gccgaccatg atcgaaatgg aggccgtgtt cgtgggggtc acggatgcgg
     9901 aggagaagca gatcggcgtg aatctggcca aggcgggcct gctggtgctg gattcgacct
     9961 cggcgggctt ccagggtgac ctcgccacgc gttcttcagg gtgggccggc acctacacga
    10021 tcaacagcgg gttgcagggc gcgctgaagt tcttcgccgg caacggaccg ggacgcttcc
    10081 agaacgctgg acacatgacc ttcaagaacg actcgcccga atggcgcacc taccacagtg
    10141 gcggcaccct gaaagtgcgg gtcgccacac gtgacgccac ttcgctggag gatgtggatt
    10201 acggactgct tatgaaggtc aaaggcggcc tgctaaacgc caaggccgcg gctctggatg
    10261 tgaacctcga actcagctat cccgtgccgg tcggcaacga ctacgacctc aagcgcaacc
    10321 gtatcgaaac caccgtcaac tgcccgatcg gccagaccat cgtgctggcg ggcatgaaga
    10381 gcctggtcga gcagtccaac atggaagggg tgccgttcct gcgcagcgtg ccggccatct
    10441 cgtggttttt ctcggagaag aatacgcgca aggaagacag caaagtgctg atcctgatga
    10501 gcccgcgcat cgccgggatg ccgatcgaga cccccgcggt ttcggaacag accgcgccgg
    10561 cgctgcagga gtccgacaaa ccggtgcaac agcgccaact cgaaaaacag aaacgccggt
    10621 tcttcttctt ctgatccgtc atggcgacac tcaccctcat cccgtccggc gggtccggcg
    10681 aagccgaagt gtacacgctg gaacccgccg gcgtgacaac gctcgggcgt tcgcccgaga
    10741 accgcattgt ccttcatgac aaccgggtgt cgcgtgcgca tctcaccgtg acgggaaagg
    10801 gaggggtgtt ccgggtccgc gatctggaca gtacctacgg cacgttgctc aacggcaagc
    10861 gcttgacgac cgaggcgacg ttgcgcgagg gcgacagcct gcagatcggc gggttctccc
    10921 tgcgcttcgg ccagggcaca aaagagactc catccgaaac acctgcggcc gacgcgcagc
    10981 atgccgcaga gccggacatc gagatggatc ccgctctggc tgccatggcc gcgctctaca
    11041 ccgaagagat gatgacgatg aaaaggcgca tccacgagca ggtgctgttc aaacttaacc
    11101 tgccggagat cgcgaccaaa caaattcagg atgaggagat gcgcgccaag ctggagaacg
    11161 cgttggatca ggtcctgcgt gaggtacgcc atgaattgcc gcgcgacctc ataaacaacg
    11221 acctgttccg gcaagcgctg ctcgacgagc tgatcgggta cggcccgatt accccaatgc
    11281 tgcgcgaccc gactgtcgat gaggtgatgg tcaacgggcc cgcacgcatt ttcgtggagc
    11341 gcaaaggggt tttatacgag accggcatgc gttttttcga cgaccgccac ctgatcacca
    11401 tcatccagcg gatcgtggag cccctcggtc ggcatgtgga tgaggccagc cccatggtgg
    11461 acgcgcgtct gccggacggt tcgcgcgtca acgcgatcat cccgccgctg gcgctcgacg
    11521 gttcgtccgt gacggtccgc aaattcgcca agcgcaaact cacgacggat gacctgatcg
    11581 ccttcggcag catgaccccg gagatcgcgc tctttctgga ggaggcggtg cgcgcgcgtc
    11641 agaacatcgt ggtgtccggc ggcacgggat ccggcaaaac cacactgctg aacgtgctct
    11701 cccaattcat cccgcgcaac gaacggttgg ttaccattga ggattcggct gagttaaaac
    11761 tctcccaccg caatatcgtg cggctggagg cgcggcccgc caacatcgag ggacgcggac
    11821 gtgtcgccat ccgggacctc gtgatcaatg cactgcgtat gcggccggac cggattgttg
    11881 tgggcgagtg ccgcggcgcc gaggcgctcg atatgctgca agccatgaac accggtcacg
    11941 acggttccct gacgaccgcc cacgccaaca cgccgcgcga ttgcctcgcc cgtctcgaga
    12001 acatggtgat gatggcgggg ttcgagttgc cttccgccgc catccgcgag cagatcgcct
    12061 ccgccatcaa cctcatcatt cagcaaaacc gtttggcgga tggcagccgc aagatcgtga
    12121 ccatctcgga ggtgaccgga cgcgagggca acaccattct cctgcaagac attttcgtgt
    12181 tcgaacaaaa agggttcagt ccggaaggca aggtgctagg ccagatggtg gccaccggca
    12241 atattccgca gtttgtcgag gcgctcaagc aacgcggcga cctgcggctg gatatgggcg
    12301 tgttcgtgcc caaagcgtaa acggaaaacg cgcgtaagcg aggagtttcc catgaccgat
    12361 attttggtgc tggtattggt attcgcggcc acgacgtggg gcgtgtgggt agtgaccccg
    12421 ctgctggcac agatcgggct ggagcggtcg gccacccaac acatccggtc cgacggcgtg
    12481 aagcagttgg ggccgatcta ccggttcacg accccggagc gtctggcgca ggcctgctgg
    12541 tcggccgcgc tgttgggggc gggtcttgcg gcagccgcgc tgatcgcctt taatgtgctg
    12601 agtcccatgc tgatcggcgg cgcctgtctg ctggcgggtg tgatcgcctt tcacatcccg
    12661 gccaaatggg tacagcaccg tatccggacc cgccaacgcc tgtttgacgc ccgcctgacg
    12721 gatctcacgc tcgggttggc gaacggcttg cgcgccggcg cggcgctgcc gcagagtctg
    12781 gagattgttt cgcgcgatat gggggggccg atgacggaag aactgatgct tgttctgcaa
    12841 gaataccgtc tgggcatgga cctgcccgag gccctcagcc gcctgtgcga gcggatgccc
    12901 ggcgaagacc tcagtctgct ggttacggcg atccggttga ccatgcagtc gggcggcagt
    12961 ctggcggagg tgctggaccg gatcaccgac accatccgcc agcgcacgga gttcaaggac
    13021 cgtctgctga ccatgaccgc gcagggacgg ttcgaggcca tcgccatggc ggcggcgccg
    13081 ctggtggctt tcttgatcct cttttttctg gatcgcgaac tgatgcagcc actgctccag
    13141 accaagatcg gctggtgcgc gatcggtgcg gtggtgatcc tcgaaaccgt ggggtttctc
    13201 gtgatcaaca aaatcgtcac catcgatgtc tgaggagcaa ccgcatggac gtaatcatca
    13261 tccaaatgct gacgttttta tcggtcctgt ttctggtcaa cggaatccag gccgttatcc
    13321 gcaaggagaa cgagacgctg agcaacgcca agccgctggt gttccgcgtg ttccatcatg
    13381 agatcggcac gatcggccgg gcgaccggcg cttggattga ccagacgttt ccggtgcaga
    13441 cgcgccagat ccgcaacgac ctgattgcgg gcgcgatgcc gatcgaatcc gtcgaggtgc
    13501 gcgggttgca aggattcgcc ggcgccgtgc tcttcgccgt ggcgggtttg accaccttca
    13561 tcctgacgct gaactgggcg tacggtctgt tggccgggat cggcttcggt ctgatgggtt
    13621 ggtactatcc cgtggcatgg gtcagccgat gcgcacgcac gcgtaaggac ctcatgtcga
    13681 agagcatgcc gtacgcgatc gacctgatca cggtctcgat ggaggcgggt caggatttcg
    13741 gcgcggccgt gcgcaatctg gtgcatgacg gaccgaaggg gcctttgcgg gacgagttcg
    13801 gtgtgacgct ccgccagatc gaactgggca aatcgcgcgt cgaaggcctc aaagccatgg
    13861 cggaccgggt ccagcttgat gagttccggt cgctggtgac ctcggtggtt cagagcagtg
    13921 aaatgggcgc gagtatttcc gcgacgctca agattcaggc ggaggagatt cgccggcggc
    13981 gttaccagcg tgccgaacgg caggccgccc gcgcgccctc gctgatgctg atcccgaccg
    14041 ccctgttcat tttgcccgca gtcttcatca tcatcgccac gccgatcatc atccgggcga
    14101 tcgaatccgg cgtgggcgag tatttccaaa gcgggggagg ctgacagtca tgttctgtct
    14161 gaaagtcgaa aaaggggagc cggcgggcga ggtccacatc ctgaaggaag gagagaacac
    14221 gctcggacgg tcgcgctctg ccacctttcg tctcggcgcg tcggatgtgt ccgggcagca
    14281 cgcgcggatc gtggtgcggg gcggcgtggt tacggtggag aatctgagcc agttcgggac
    14341 gaaagtagac ggcaccccca taacgggaag cgtgcagcta acggaaggcc agcgcctttc
    14401 catgggggaa agcacggttt tgtgtctgtt gcggacgccg gcaggcgaac cttccgccgg
    14461 tgccgatatg tcggccgccg agccggacgt aacgcagacc aaagggaccc tcggcgtgga
    14521 agcgatactc caggcgcgcg ccgatgagat ccccacgggc gaaggcgtgc cgccgtcccc
    14581 tgaaacaggc ggtgcgaccc gcgccacccc ccctccgcct ccgccgcctg cccaagtcgc
    14641 tgccgcagcc cctaccggcg cggagttcgg tgcgcccgtg cctgcgtacc cagactccga
    14701 tctcacctcc ggcctgccgc tcgaagcctt gacggtcgat agcgagacca tcgagggcgc
    14761 cacccgcgcc atgcagacac gggcggccac accggaggag attgaacacc tgcggatcgt
    14821 ggagcagaaa cggcacaaga agcggatcac gcgcgcgctg gccgtcctga tcccgctgat
    14881 cgccctcgtg gtgatcttcc ggccccgcac accgccgccc gagccgaaga tcgagtgggc
    14941 gaaagacgag gagggcaact acctcgacct ctttgaggcc gcgcccagcg gcggacttgc
    15001 ggaaggcggg tatgacctct gttaccccgg ccccgtccga ttcgataaaa ccgtcaccga
    15061 agacggtgtc atcctttccg ggtttgtcgg gcgcgaacgg aacgtgccca tgcggctgat
    15121 cacgcaagag cagaccgatc cgaaattcgt cacaatggac cgcctcgcct ttgtcgagga
    15181 atggatccag cagcagaaag cagacaacaa gcgctggagt tttgacaagc cttcgccgat
    15241 gcccggcttc atcggaatcc gcaacggaat cccctacctc cgcgtcacgt attaccgcga
    15301 cgacgacgag ggttcctggt tcggtgtggc cagtgtgttc cgttacgggt accggcggat
    15361 cgtgatccgc gccgaagccc ctgcgtcgga acgcgtgcgg ctggaggatc tgctctcctc
    15421 caagttcatg cgcgcctcag aggccatgga gtatgcgtat tgggaagcgc ccgaggagct
    15481 gcccaaacag agcgaggccg tgttgctcaa acaggcggag gcggatctca accgcatggc
    15541 gccggcaacc tggttcgagt tgcagcgcaa cctttcggga ttgttggccc gcaccgtatt
    15601 gtccggcaac aagaccacgg aggtagaggc gttgcgtctg ctggtccggc tgcgtgagcg
    15661 actcaccctc tggttcaaca gccagcagct tgcctttgac gcggcgcgta tgcaaaataa
    15721 ccggggtcaa gcgcgggaga ccgcccggct gacccgggcg gtgttcaagg acatggagga
    15781 ccagcggtat tacacggtcc gcaaatggaa cacggagttt taacgccatg ctgatccgac
    15841 tggaacacaa cggacgcatc atacacgaga tcggttcgga cgcgctgacc gctgaactga
    15901 cgatcggccg gagtcatgcg tgcgaatggc cgatcccgaa agaagatggg gtcgccagtt
    15961 cgcgccatgc agccctcttc aaaaaaggcc gcgcggtctg ggttaaagac ctcaaaagca
    16021 ccaacggcac ctttcacaac ggcaagaaga tcgaaaaacg caaactggcg atcggcgaca
    16081 aaatcagtat cggcaattgc gtgctcagcg cggaggccga ccggagcggg accggccgca
    16141 atctctcgga ggtggtcgtg ctctccggca aaacgcgcgg ccagaaaaaa cggctcgtgc
    16201 cgccggtctt cacgatcgga tcggacccct cctcttcgct ggtctttctg gacatgttgg
    16261 tctcgcggcg ccacgccgag attctgatcc gggaggacgg cagttgctgg atccgcgatc
    16321 tgggcagcaa aaacggcacg tcggtcaacg gcatgccgtt gcgcgatgac aaagagcggc
    16381 tgctcaaaga cggggactgc atcgcctgtt cccatttcga actcgaattc cgcgacgggg
    16441 cggttaaaca ctccaacaaa cagacctggt tgcggttagg catcctgatc gtcaccctgc
    16501 tggcgggtct gggtgcctac catacgtatc aacggctcaa accatcggcc gaggcctttg
    16561 tcaaagcagc gcgccgactg gccgccaaag aagagttcga tcaggcggaa cgcgaagtgg
    16621 aacaggcggt caacgcgcgc cacgcatcgc gtcatcaggt ggcaatcgaa gagatgcgcc
    16681 ggttgatcgg tgtctggaag cggacgcgcg cgctctggca gagcgcgaag gaggatctgg
    16741 agcgcggtga gtgggtcgga gcctcgcgca ccctcggcgt gctgcaagcc agcaaacgcg
    16801 aggtctggga gtggaacgcg caggccgccg aagccaagga cgccgcggca tacgccaaaa
    16861 ccctgctgga cgctttcctg cagaccgacg cgctgctgaa gcgggaggat ctggagttcg
    16921 ctgagttgga aaacaatcag cccgtacttg accgcgtact cgacgagaaa ccggcgacag
    16981 ccgaaccggc cgcctatctg aagcgtctcc tcgatgaact gcgccgtgtg caaacccggc
    17041 aaaccgccct gctaaccgag agtcgtcaga tggaaacggc cctcaccaaa ctgggcgagg
    17101 cccaaccgtc gtatgatgaa gtggtgagcc tgctcgaaca actggcgacg agtcaggaac
    17161 ccgccttgag acgccgggcc caagtcttgg tcgtgccggt gaaggccctc gcaaacagtt
    17221 acatcaaact cggcgaggcc gcgaccaccc tgcggagcct cgcgttccag tcggcactga
    17281 agctcgacct ggctctgccg acagtgaatg cctgtgccat cgagccgcgt gtctccctgg
    17341 cgcgccagaa catcgagaag atccacgcga acctcaaact caaggggtat cagctcgact
    17401 ccctgttcac ggaggtggaa aaacggatcg gccgtaacga cgattcgctg gaggggttcc
    17461 gggtgctgcg taattcggcg atgatggaga aggtgatggc ctgcgattcc atgaacctgc
    17521 ctctgcccaa gcgcagccgt tcccagccag ccggcgagta tgaccgcctg ctgggcatcg
    17581 aggcgttcta cgcctatctc agcgcacagc ccgaaccgct tgaccccgcc atgctggcgg
    17641 acctgccctt tgtgccgctg atcaaccaga cacgcgaggc cctgcagaag atcgacacct
    17701 ttctgcagtt catggcgcag gcggacaacc agtggctgct ggacgatgcg ctggccgtgc
    17761 aagtcaagcg gatgaaagcg atcatgcaac agcgcgacga gttggtgaaa ctcctgatta
    17821 cgcgtgccgg gtccgccacc gaccgcaccg ctctgatcgc gggcggcctt gccgcgcgcc
    17881 tgatcaccgt ttccgggaag accaagatca aagacctgcc tccggaagag tggctggcgg
    17941 ccgagctgaa gcggcaacgc accgccctgc tgcgcatgaa cgaggagtac acgctggctg
    18001 ctccgacgcg gcagatcgag atccgtgccg cagtcctgaa agccggtctg cccggtgatc
    18061 cggtggtccg gcgcatgtgg gccatgcgtg atgctgcggc agccgccatg tcgtccacac
    18121 cgtaaccgct ccggctgaaa acggcgagac cacactccca caactccccc tactggagcg
    18181 cctgtttgat atagggcagg tattgccgcg tatagacgac gatggaccag aggttcagaa
    18241 taatcagcag ggcttcaatc aggtagatca gcgcctgcgg caggaaccac gggtggaggg
    18301 aggcgtagac gtagatcaca cacgcggccg ggaagcttac ggccgtgcgg aacttcccca
    18361 tccaattggc gcggcagtcg gcctggaaca tcgcgcccac ggagcggaga aaggtcaccc
    18421 actggtcccg cagaatgtag agcaccgtga agatcagcat caccagcgcg tgttcggcct
    18481 cgttcggctg gcgccccagc agccagagca gggtggggaa aaccaccaga taaaaaacct
    18541 tgtccatcag cgggtccgcc atcgcgccga accgggaaac cacctgccac ttgcgggcca
    18601 gtttgccgtc gcacagatcg gtcagggcgg caatggacag cgatgcggtt gccgccaccg
    18661 ccaaccaaac gccgcggggg gccatatacg catgcgtcac ggccaaaacc atgaagagca
    18721 ggacgaacgg cacgcggccg aaagtgatcg cggtcacgat catccgtttg gcggaaacat
    18781 ccatgggaag tatcaaacca taactcaccg ctcttgaaaa ctcaaaaccg gcgcgtccgg
    18841 cagatcgaac ccgaagtacc gttccgcgtt tcgggtacag atgtccttca cgatttcgcc
    18901 ggtccaggcg agatcgttgg ggatccggcc ctgcgtgatg tcggtaccca acagattgca
    18961 gagcacacgc cggaaatatt catgtcgcgt gtaactgagg aaagagcggc tgtccgtcag
    19021 catgcccacg aaacggctca gcaatccgag ctgggagagc gcctccatct gacgcagcat
    19081 gccgtcgcgc tggtcgttga accaccaacc gctgccgaac tgcatgcggc cgggttcggg
    19141 accgcgctgg aagttgccga gcagcgtggc gaccatctcg ttgtcgcgcg ggttgagggt
    19201 atagatcacg gtccgcggca gggcccccgc ctgttcgagc cggtcgaaca accgtgccat
    19261 cggtgacgcc acatgccagt ccccgatgct gtcgaatccc gtgtccggcc cgagccgttt
    19321 gaacatgatc gtattgttgt tgcgcatcgc gccatagtgg atctgcgcgc tccagtcgct
    19381 tttcgcatcc agcgtcaggc tctcgtagag gaaggcggag cggaaagcgg agacctcttc
    19441 gggcgtcgcc ttgccgccct ggcgcacgcg atggaaaatc tcgcggattt ccgtatcaag
    19501 gtacgggtcg gcatataccg tgtcgagccc gtaatccgag aggcggcagc cgtgcgcggc
    19561 aaagacctca tgccgccgct gcagggcaca gagcagatcg tcccaggtct tcaccggcat
    19621 atccgcggcc gcagagagcc ggtcaagcca ggcgttccag aacgcgggcc ggtccaaagc
    19681 cagcgctttg tcgggccgcc aggtcggcag tacccgcacc gcgaaatttt cggcgcggag
    19741 agccgcatgg tgctccagcg aatcggcggg atcgtcggtc gtgcagacca gccgcacgtt
    19801 acttcgcacc atcaggccgc gcgcgctgaa cgcgggttcg gccagttgcc gtccggtttc
    19861 ggtccagatc gcctcggccg tggcaggggt cagcagatcg gtgataccga aataccttgc
    19921 gagttcgagt tgcgaccagt cgaagagcgg gttgcgcaac agatacggca acgtctccgc
    19981 gtacttctgg aacttctcgc gatccggggc atcccccgtg atgaaccgct cgtccacccc
    20041 gttggatcgc agcgcgcgcc acttgtaatg gtctcccccc aaccacaagc gggtcatgtt
    20101 ttcccaacgc cggtcctccg caatctccgc ggggggaagg tggcagtggt agtcaaggat
    20161 cggcatggtc gcggcatagc cgtgatacag ctctcgcgcc gccgcattgt caagcaggaa
    20221 gtcgtcgtga atgaacggtt tcataacggc acgcatttaa gcacactcgg cggtgcaaga
    20281 gcgattcctt tttatttacg gcttttgtct tccggcctcc gctcccgcct gcggagtccg
    20341 gcctgcggat ccggcctgcg gttatcaaca cggcgcccaa aaatcaccca aagggttata
    20401 aacaggtgat aataagcctg ttgataagat ataaacctct attgttaagc aagttggctt
    20461 tatcaaattg tgtagatatc ttgtgcttgc cgatccagcc gggtttggcg catactagcg
    20521 gtacgttgtt atcaacaggt tttgtttagg cacatggcac agctttcgat aactttttgc
    20581 cggaaattgg cgcgggcgcg gcgcgaacgg ggcatgacgc agagcgcgtt ggcgcaggcg
    20641 gtctcctgtg cgcaatccgc gatcagcatg ctcgaatcgg gccagcccgg aaaactctcg
    20701 caggagacgg tcggcaagat cgcctctctg ctgggcgtgc cgctggagga ctccgcaccg
    20761 gacggcgtgc cgctgccgac acgcccgagt gcaggttact gtcccaacgc ggcctgcttc
    20821 tcgaacatcc cgtatgtggt ggcggatgag gtgctcttct ggccgcgtcc gcagccgacg
    20881 gcggcacggg ggccttactg cgtttactgc ggggaggtgc tggaaccgca gtgtccgcat
    20941 tgcggcgcgg gtgtgacgcc aggggcctgt tgcccgcaat gcggccgcgc gcgcatcacc
    21001 ggtggcgcgc cggacggcgt tgaccctgaa gattgggcgg cgcggcggcg gcgtgaactg
    21061 gccgactggc gcgcgttaag cgcttggacc ggattcgctt cggaatgacg gggataggac
    21121 ggaacggaag aaagagagga tcagagaccg catgactaag aagctcttga tcgtcacgga
    21181 cgggaaggcc ggccacgaga accagtccaa agccttttgt gcggcattag gatacggcta
    21241 cgagtgcgtc cgcgccgttt acccctcccg tttccacaag ggactctcct atctcatcga
    21301 ctggctgggc ctgctgctca gttccccttt cacgatcgaa ccgaccgacg gttatttcgc
    21361 agccgtggtt tgcaccggtt cgacggcctt ctatcccggc aaaatcgagg cgcggcggcg
    21421 cggcatcccg gtggccgcca tcctctatcc gtccggatac cggaaggcga acttcgactg
    21481 catcctggcc ccctccttcg accggccccg tccggccccg aacgtcatcc cgatcccggt
    21541 caacctgacc tcgacggacg ccgcgtttta cgagacgggc gtggcggcgt tccgcgcgcg
    21601 gcacacgcag cgcaaaccgg cctccgccct gatcgtgggt ggccccaacg ccgcggccga
    21661 actttcggtc gagaccctgc gccgcgagct ggaccggttc ttcgccgtca cggaagggaa
    21721 cgaacgctgg gtcaccactt cgcgccgcac cccgcccgcg attgaggagt tcatcgcctc
    21781 ccgcccgttt gactactcgc tgctctacag ccgcgacacc ttcaacccca tccccgcgtt
    21841 cgtttccctc tgtgaacacc ttttcgtgac cgccgactcc accgggatga tcagcgaggc
    21901 ggtcacccgg ggccgcgccc gcgttgaaat cctgatgaca ctgcgcaaac cggattccaa
    21961 gttcggccgc ttcatccgcg atctggaaca ggccggagcg gttcatgtgt ttgacggcac
    22021 gcccggttgc gcggaccgca agatcgatct cgcaccggct tttgaacagg cgcgcaagct
    22081 gctcgggctc tgaccccgtg ccgattacga atccggccac aaacagcatt gcactttttg
    22141 cccctgcttt tcctgaagat tccgttttag ggtttcaggg tttcatccaa atcgggatcg
    22201 gggtcgggat cgctatcggg cttgggtttc agggtttctg gtttctggtt tcatgtgttt
    22261 catggcttca tccaaatcgg gatcgctatc gggatcggga tcgggatcga ttgtttacca
    22321 cgaac
//
LOCUS       DAOSRB010000021        21833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2588_length_21833_cov_6.245890, whole genome shotgun sequence.
ACCESSION   DAOSRB010000021 DAOSRB010000000
VERSION     DAOSRB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21833
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_21833_cov_6.245890"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLZ74_02295"
     CDS             <1..402
                     /locus_tag="PLZ74_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97183.1"
                     /translation="VGLAQTNFYEKVSSMWYSGNKTGVLSIAEQRLQQNTNDIAGLIL
                     KMEYELEFLNFEAMTNTMQRVVAVGGGVSTTNFVVEFPSLQGSINHLLNMIPLYPVNE
                     IAVDREKGKISGKPLDFGDAIKALQDDGYFQ"
     gene            1000..4032
                     /locus_tag="PLZ74_02300"
     CDS             1000..4032
                     /locus_tag="PLZ74_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOR97184.1"
                     /translation="MRTSVIGTVMVVCAICVQGATKFWTGEGADDKFSTDGNWEGMAA
                     PVNGDTLVFQGENAVAENDFDSTVQFAAIYMSNSVPFTIRGNTLYLSGNRIFAKSLQS
                     EITDRFEADVVFSGGASLGSDSVTKHHITFGGTVASASGGTLATATQTGNIITFEGMV
                     TNFAYVYRPNGNGTLRFFAPTNHFTNPSSFQINQGTITVSNLTAFGTDTRTFSGGQGR
                     YNTPAIIQMYPTADETAPIHFKVYSPNQQQSNLQLYNNTPGVTLTFTGDVSLGWTVGN
                     GGRIAVGGAGDGMITGDFTSPNLALYKSDTGIWELAGAYASTGVVTVAAGTLVVNTVL
                     PTNTLCSVTAGLLCGTGTLHRAILSSTSQITGGVPGMPGTLHFANGGEVALTVASGTL
                     VARPKADGTPSLVAVDGTVALTGLLTVNLDGDFSALPAGTYPLLTWTEKTGTGTYAAG
                     SGFPSGSSLVETAAGLALYVPGAVLSWTGAVNGDWDTATANWNSGQVFADGTDVVFGD
                     DVVNGQTNVTLTTAAAPGSVTISSDVNGYTFSGAKMTGTMTLLKTGNASLTLENDNDY
                     TGLTTINGGTFRLNGSLDGSPLLVQPGAELMQGTNASIRGDVNVNLLGQATLSGSNTF
                     TGDLVFGSIAEGKATGTVCNVSAIGGDGGNLVVMHNARVMLDAEGVYPNRTLRFAARQ
                     SGAGTVPYLKQMSGKTATWPGGIAFDAPAYDYSFEISGTLNLGTDPDAVITDAGSASA
                     STLMVRGTGRLNQYARAQLKGGFGKTDSVSWHVYASNNVLSQFNISVGSAFLHAPNAL
                     STNCTIKLGQVYGNNKFNSLFDFGGFDLAIAGLAVDQGISIEGNTQVFTTSEPATITL
                     LGPGDTILNRTGTELKGALAIRKRGAATTLFLGQPNSNTGDFRLEEGYTVASTNRAFG
                     YSTNLFVGATGTLTVSASDALPPEDDVWLRVADGGTVVLSNDVAATIFGLEINGRPRG
                     AGTYGGEGSGARIILPAVFSGTGTLSVRNGAGTILRLH"
     gene            complement(4024..4146)
                     /locus_tag="PLZ74_02305"
     CDS             complement(4024..4146)
                     /locus_tag="PLZ74_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97185.1"
                     /translation="MIRITAKMSTEDVGKVIDELMDGAVAKAQRALIGAPAALV"
     gene            complement(4209..4724)
                     /locus_tag="PLZ74_02310"
     CDS             complement(4209..4724)
                     /locus_tag="PLZ74_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOR97186.1"
                     /translation="MSGLWQKIKDGFVRTLEILLMGMVAVMTLTVLWGVFTRFCLGHQ
                     AHYTDELARVLLVWISMIGSALAFGAKAHLGVDYFVSKLHPEARKTMSVVVQLVILAL
                     ALIVFVIGGWGLAMGQMGQQLPALPIKRGMVYVSMPIAGVLIILFALENMVEIIRTPA
                     GKLGAQTKSEG"
     gene            complement(4780..5019)
                     /locus_tag="PLZ74_02315"
     CDS             complement(4780..5019)
                     /locus_tag="PLZ74_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOR97187.1"
                     /translation="MLLQGLILMVVGVGTVSGFLGLLVVILTLSSKIIPRFNHILPDE
                     QAKVKAQKAQKRDASPDAEIAVAIAAAMLRQRAHE"
     gene            complement(5039..5290)
                     /locus_tag="PLZ74_02320"
     CDS             complement(5039..5290)
                     /locus_tag="PLZ74_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97188.1"
                     /translation="MILLAYLSQNRPRFPSLPDVAYLLVLVSLVAARYIDIRRFNGAN
                     GENSAPATMADWRRYTLFLVTGCAVVWLIIRVLVPALAK"
     gene            complement(5708..7198)
                     /locus_tag="PLZ74_02325"
     CDS             complement(5708..7198)
                     /locus_tag="PLZ74_02325"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOR97189.1"
                     /translation="MKKNWGMTLLRMLIGWHFLYEGVWKLIQPGGWSCEGYLRMSQWF
                     AAPLFRHIANTPWLLRLCDLANMWGLTLIGLALILGVLVRPAAVAGMLLLAFYYIAQP
                     PFLAYTSEGHFLWIDRNVVEAAALLAVCLVPSHQIGPILWNRLCLFLRRRDIAADGGS
                     GLGVSSAGRRELLLSLSSLPVIGAFGYAFSRKHGLAWERENLKTTDAVTSATVKSFSF
                     DDIKDLKKPVDKFGQIGNLKLSRMLLGGNLIGGWAHARDLLYTDKLVKAYHTDERVFR
                     TFRMAEACGVNTISTNPALMRVINDYWRKEGGKIQFISDSGHKDGLVRGAELSVENGA
                     SAVYCHGGRSDNLALKGQFDKIGEAVEGMRKLGVPVGIGAHRLETVKGCVENGIIPDF
                     WVKTLHRTEYWSARPEAGYYSRDNVWCLDPDDVIAYMEKLEQPWIAFKILAAGALHPK
                     DAFPYAFQSGADFICVGMYDFQLVEDVNLVNTVLDNPIKRSRPWRA"
     gene            complement(7323..9587)
                     /locus_tag="PLZ74_02330"
     CDS             complement(7323..9587)
                     /locus_tag="PLZ74_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97190.1"
                     /translation="MKDRLYLTLGAIVAFSACAEEMPKTPFDGTTPVTVETKTFRLII
                     NPDATAKSLIVKATGEEMLEPREGIPVFASTQIRPFNNEIRLVQQAKRTTYPANRVRR
                     EGDLIFIGFETAPYQIAVRVTETAAGYATFRPERLISNTTDEHQYYHWNLDVPPIDAF
                     RLLQLPVKNRANFGDWLNVMWDDQAFVGVMGGTPYMDVDNERRHGFRKLTVESLKDYG
                     VTNGVAVLAVESQKERFLDQIDAVERDLGLPRGVQSRRDSRLNASLYWTAGDFRPENV
                     DALLSYLKKGGFRMLLVYFPGIVKAKSYNTLPDYSFDNGWDAEKLTAALGRFHKEGVS
                     VGLHTLQTFIGFKSSYVTPEADHRLALVKRFTLAKPLPLSEEPCEVFVEENPVAAPMH
                     PKIRILKFGTELMTYTGYTTTRPYTFTGVKRRHLGTYAKAHPLGEIGGVLGVSECAAI
                     SAYVDQDSSLQDEVAEKIAGVYACGFDFLYFDGSEDANPPCTVNISLSQYRCVNACTA
                     RAGRAPLFTQGCAKSHFGWHFQSGANAFDVFGPEIFKKKIIEYPYAAAQRLAKDFTRV
                     DFGWWGCWPARLDGPPPAKEGLFRSGNRTVGTQPDIWEYGTSKAAAFDCPATMQLSLD
                     TLKKHRRTDDLLETIRRWEDVRARKWLTPGQKELLKDTTKEFHLYRNSRNEYELVEWR
                     QLDVAGDKWSRVRAFVFERQGRRVVAYWDIADRSELVLANGVGTLKAENLIYWETQMN
                     EADVHAAFAGATIR"
     gene            complement(9608..11557)
                     /locus_tag="PLZ74_02335"
     CDS             complement(9608..11557)
                     /locus_tag="PLZ74_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97191.1"
                     /translation="MLERRGGCHWFWDIDVVPHKEDIDLTGLDVNEEARFEYRGLRYF
                     AHRGLTRFQAEHWGLDDWKREIDWCLKRRLNVFMLRIGQDDLFQRTFPDIVSYPDASK
                     NLPGTGKGYDNRTLFWSLEYRGKLRHDLQQYAFARGLMVPEDFGTMTHWYSRTPEEFL
                     EKMNPPFLPQTTAGYSEKNGLVWDVRDDKWVDAYWKLTQTAVEAYGQGKDRQQLLHTI
                     GLGERRCYTNRQENLELKIASLKKFLARAHRDYPNAKTLLAGWDFYFTWRPEEVQELV
                     RSLDPKRDIIWDYEGDSTRDYRPEMLDLGGNNFTKWGVVGKFPYTFSVFLAFENALDV
                     RANYPLIEERQKIVQDDSACVGYIFWPESSHTDTLLLRFFTANAWSSTSVSHRQVLDE
                     FCNSRYGALAPRMKSLWEKVIPVSYMSDWGSNYSYLFTRKSFGTKQYGPAGKTDAETW
                     GRRLAEAKGVFAELAKIEWQGEAVRRDTIDLARTVLDRMMVMEAGTLAADFEKWQGGA
                     ENGAKMIAAANRLAGLADRMADLLELHTDYSLWESYLRLNEIEPVRNPNFPSVLFENA
                     ANTYCQSHQYELARYWYAPRAHEWAAKIETIIRSKDRKSPLQLESGEVLRNELQKRPL
                     ETLRPRSDRSPKVFAALMEVLSESP"
     gene            complement(11881..15618)
                     /locus_tag="PLZ74_02340"
     CDS             complement(11881..15618)
                     /locus_tag="PLZ74_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97192.1"
                     /translation="MKLSQTRGKGRSAGAVRGGMAMFAVAFAQMAVAATDYVWTGSSA
                     SGPVSMPDWADEDNWNPKSIPNSDDALVSVSGGWVSNTMPVTVARVENAGSTTRYISD
                     HQFSIVSNLFSKNMAFFAPLHIKGSTPYLRNCSLAGDMTFGNGAKILVHGGTFSINDN
                     QYAPSSETTWRPSPLGTITEYHGSLSFNGPRGSSAAITQKFSTQAGSPFVKAVTAGAA
                     DTLPIGALVSGAGVSGGTFLRRIFGKDDFVELSQPVSETAAAAELSFAGRKGDCRRYV
                     NELAHNGSTDHNFTINLGKYRMEDIYEVEVGLLKLLTLSGGNRWTRIHCNNTFPGRLI
                     LHDTPKVLNTALEVGTCDILFSDAPNTENGPGLPYATVRQGDAGQNSRFSVTGNMEAV
                     IHEFQAIKGSLTKGGAGLLRVGFGEGPHTGALVIEEGTFVPTAEDESCPIEIGSFSIT
                     SNAVLQLPVQGLRVTNRVGSLAGGVVKGPGKLFFSNVASIDGLTLEDGAEIVFECDGS
                     IIRSEGGETVPGNPAFWVDCSKNGSAMTLVDVTNGVKGVTRIEDVRQKNDPDGDYIYS
                     EVCSAPGSGYFPELRTDTTRNIQYVHFEGNFVSGSSSPADSDTHRWSKPVKNIRHVFQ
                     VMAQDRWRSGGNFLGIIDTQQTGTEAEAWAHYNVYTGKIFDWGTDAIKQGKLWINGVL
                     SNPDQGYPYPAYENASVKYFVPIVLAFECNANYELPGADSYEYNTVRAKEGRIGHKCL
                     GELIVYTNALTVVECKQIMAYLTRKWQTTEICYAPIQTGSEASLGTLNLGEGSGGIGV
                     KDGAELMVDRVTGAGTLAKTGNGTLLVPALPEDGSVSFLVKEGTLEIQSVAATENMLP
                     AGAWARYDASNLNNLEYTVENGVTNILKWLDSASGEHPASHPDYCTNYPTLRTSTALG
                     GMPYVDFGEAKRWEKGGLPTNQYAPSAPVLSYASTPQLHTVIAVWGADHGGGNLVGTS
                     APDSQYWSSVYTIVRAGETSGPLGGDRDQPVVRSDMLMPKTAMDERGVRFHKNGVACD
                     PRQTGLSAGFNIVSWASYENFGGSALTGVASSTRYAGGEQFAEYMLYEKQLSTREVAL
                     VEAYLRQKWFGQPTPGWNGTKASKVAVSNGATLKVVGNAPLSVETLSLAGGTISGGVA
                     LQSAADLEAAVNADGTVTPVTVDGTIDFSKGGTVTFTGATNMLTYGSYVLLTGIGECD
                     LSNWSVDTAGLPGRYQNIVLSAAGGSLTVKIVPGGTSIRMK"
     gene            complement(15699..16277)
                     /locus_tag="PLZ74_02345"
     CDS             complement(15699..16277)
                     /locus_tag="PLZ74_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97193.1"
                     /translation="MYDVIAPDAKSQKPEELLAVMKSYKALVVAGDYPDTSWEKTLAR
                     YEADGGKVIHVGVDMLADPVKIGKGRGIGNGETRYPKLEKALNDLEAEYFPFEVEGDC
                     MYGLTVADRHSWLYVFNNDGVTKFADAPQELDATKTSDIRITFKSDKKRLCAVRELLS
                     EAVVAVSAGVSFDCRLGPGEFAIFEITSAGLK"
     gene            complement(16339..17748)
                     /locus_tag="PLZ74_02350"
     CDS             complement(16339..17748)
                     /locus_tag="PLZ74_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97194.1"
                     /translation="MKHAQVRYVFPRGLLAAGLGLVVLTGCDERDVCGVPLVEVPARH
                     KPLEIMGRDCWRVLTRYTPYGWSGKLLAADNPEFNNSLAISDVVPKYVAAAEPPAAAT
                     GYAVWPTLDLHFRPDAPDREILTSNPHPDRPFFLSKRGERNRGTWGSPVQLDKAAYAA
                     WRAAHPNLVADGACSEWCNDLNNAWSSVAGEKGTYVDHRPDSSNRVAILRAFLGEKPK
                     NRYEHVALLKRYYEMRKERNYGGTMSVLDAHLNSLHIAADFGAVLLRMETTSSGQYRY
                     QPSAMFTRGAARQFGIPWEWYIAGYANGPSAKGRGFLGDAMCRYPATPEATGEPRYAK
                     EREASWCPGGKIRIGNSGPDFGISRSLFKRTHYLAYLSGANYIELEEWNCGILNMWDK
                     EKGRTVFSPRGEIYAAFAEFIKDHPDRGTHYSPVAICVPLAQGYPTWGGGAVRRSLVR
                     LHRRRQGGRCRLLYARTRL"
     gene            complement(18170..19972)
                     /locus_tag="PLZ74_02355"
     CDS             complement(18170..19972)
                     /locus_tag="PLZ74_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97195.1"
                     /translation="MCTCGADKYLWTELIAFDNTEPDYGVQAYLDKMSVKPKGVSFLI
                     FDPELFHAHTDLSSDFQIGDLHCSYFARPWNEERLRQKWTAWQFRGLIAELKKYGIAS
                     YPSFFDMAPGKGNSWLRQFKATRKDVTWFDRHPEVGYRMKDGRRANNVNPIERLADGR
                     DYADFFVAQAVQFLTDYGFAGLHACDGFGHPRYPLNQLDYSHLFPEAKDRASLCRAHA
                     ARHAAFIAKLAAALHARGLKLYANTSWTRDPLEALYRHGIDYRLMEKAGLDGFMAEAS
                     AIVLELEGWRFSPVPLLDSCRVAFLLTGATVDTPIIHLACVKDGMEQYNSLRDAPQRV
                     TAEVVLLGNLLRGDRLMVPDVLWCLTDGFTREEWRRLDTAWNLAKCPKRADGVRVVVS
                     SHAMDAEFDAYCSGGYPSAYTLLAHLVNAGAAVNSAVTVEEAKADADLPLLILNSGRF
                     PADELASLTNRSAAVVTFGYGSPACPFGPRPSVLDAASWRHPLPFRAFDPQALAAAAE
                     KVNRASPVFPGADMADLRLSSYVAEDGSRIVFAVNDRPTYLNANILVRGPVTTAEALT
                     ESPSLPIVISAKAEDLTSLVAKIPPAGIVVLRLK"
     gene            complement(20025..21422)
                     /locus_tag="PLZ74_02360"
     CDS             complement(20025..21422)
                     /locus_tag="PLZ74_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOR97196.1"
                     /translation="MKILVGSFQCESNTFSPPKAGKENFYILHGEEAKNKLFASHLFE
                     EAGFEVVPLVYAVSLPSGMVTRECFDEVLADFRKEAAKHSDAAGIYLYLHGAMCVEGL
                     GSGEEFTVKELRKTIGDQIPISLAQDFHGNLADGLIPNVQAISGYRTAPHTDYDETET
                     RAAVALMRILNEKAVTVPHFFRIKVQFADAAQTSVEPYVSIMKMVRELDTDPDIISAS
                     VFNGQPWVDAPCMSASVIVYSKANHEAVHAKAKAIADYFDAHKTDLKFGVPALAPDRT
                     VDAVKTMAKPVFVSDSGDNTTAGAGGESVFLLKRFLGSDVKTLFTSLYCPTAWAKLTE
                     MKPGDRATLTIPKSDEYNDDLMIEGEYIHGAKILGFVGEESGEGILFRAGHVDIVFAS
                     VRTSFTMKEHFAAMDVAAADYDAVVVKQGYLWPGAAELAASQIFCMTPGTATNDFATL
                     PFKNLEGKYYYVRPQ"
BASE COUNT         4673 a         6706 c         6039 g         4415 t
ORIGIN      
        1 gtcggccttg cgcagaccaa cttctacgag aaggtctcgt ccatgtggta ctcgggcaac
       61 aagaccggag tcttgagcat tgccgagcaa cggcttcagc agaacaccaa cgacatcgcg
      121 ggtctcatac tcaagatgga atatgaactt gaatttttga actttgaagc tatgacaaac
      181 acaatgcagc gagttgttgc agttggcggc ggagtttcaa caacaaattt tgtggttgag
      241 tttccatcac ttcaaggtag tatcaaccac ctcttaaata tgattccgct ttatccggtc
      301 aacgagatcg ccgtagacag ggagaaaggg aaaatatcgg gaaagcccct cgactttggg
      361 gatgccataa aggctcttca agatgacggc tatttccaat gatcgtcggt gcctcctgtt
      421 tattgatgga ctgcgcacca atcggcagcg caagggtttt gtctgtaaat tcgtcaggcg
      481 gggtcatacc ggcatgccct ccatgctgag atagattctc cctgtctccc attcctcgct
      541 gatctctgac agcacggttg acacgagcct gagcagcgag gcctcgttgg ggaagagccc
      601 cgcgaccccg ccccaccctc cgcaccgccc ccttctccct ctccgtccat cctcttcaca
      661 ccttactagc gcgggcggag tccgcaatct caaaggctgt gagacggtga ggctgtaaga
      721 cggtgaggct gtaagacggt gaggctgtaa gacggtgagg aattgagcat atgagaatga
      781 ataaccaaaa aagttaggat gtttgatgcg acccgtagca tattccctca cagccttaac
      841 accttgccgc ctcacccccc cattacggat tgacttctct ccgcgaaacg cacataattt
      901 ttccaatctg gatgataacc tgtcgcgcgg cgataggtaa agatgtgtgg tagggtgggt
      961 tcaccgacag gcgggtcctg tttgataaag gagaaaacga tgcgaacaag tgtgatcgga
     1021 acggtcatgg tggtctgtgc gatttgcgtg cagggggcga cgaagttctg gacgggagag
     1081 ggtgccgacg acaaattctc gacggatggc aactgggagg gcatggccgc gcccgtgaac
     1141 ggcgacacgc tggtcttcca aggagagaac gccgtcgcag agaacgattt cgactcgacc
     1201 gtccagttcg ccgcgattta tatgtccaac tcggtaccgt tcaccatccg cggcaacacg
     1261 ctttatctga gcggcaaccg aatttttgcg aaaagccttc agtcggagat tactgaccgt
     1321 ttcgaagcgg acgtggtttt ctccggtggc gcgtctctcg gaagtgatag cgtcacaaaa
     1381 caccacatca cttttggggg aacggtcgcg agcgcgtcag gggggacctt agcgaccgct
     1441 acccaaaccg gaaacatcat cacctttgag ggcatggtga ccaatttcgc atacgtctac
     1501 cgcccgaatg gtaacggcac gcttaggttt ttcgcgccaa caaaccactt caccaacccc
     1561 agttcgtttc agatcaatca ggggacgatc acggtctcga atctcaccgc attcggcaca
     1621 gatacccgga cattctccgg cgggcaaggt agatacaaca cccccgcaat catccagatg
     1681 tatccaacgg cggacgagac ggcaccgatc catttcaaag tctacagccc gaatcagcaa
     1741 cagtccaacc tccaacttta caacaacacg cccggcgtga cgctgacctt caccggcgat
     1801 gtgagcctgg gctggacagt gggcaatggc gggcgcatcg ccgtcggcgg agcgggcgac
     1861 gggatgatca cgggggactt cacctcgccg aatctcgcgc tctataagag cgatacgggc
     1921 atctgggaac tcgccggagc atacgcgtcg accggcgtcg tgaccgtcgc ggcgggaacg
     1981 cttgtggtca acaccgtgct cccgacaaac acgctctgta gtgtcaccgc cggcctcttg
     2041 tgcggcacag ggacgctcca ccgggcgata ctaagctcca cctcccaaat caccggcggc
     2101 gttcccggca tgccaggaac gctccatttc gcaaacggag gcgaggtcgc gctcacggtc
     2161 gcgagcggca cgctcgtcgc ccggccgaaa gcagacggca cgccgtcgct cgtcgccgtc
     2221 gacggcacgg ttgcgttgac cggcttattg acggtgaacc ttgacggcga tttctccgcc
     2281 ctcccggccg gaacgtatcc gctcctgact tggacggaaa aaaccgggac gggaacatac
     2341 gccgcaggca gcggcttccc ctcggggtca agtctcgtgg aaacggccgc cggactggcg
     2401 ctttacgtgc cgggcgcggt tctgagttgg acaggggccg tgaacggcga ctgggacacc
     2461 gccacggcga actggaacag cggtcaggtg ttcgctgacg gcacggacgt tgtgttcggc
     2521 gatgatgtcg tcaacggaca gacgaatgtc acccttacca ccgcagccgc accgggttcg
     2581 gtgacgattt cgagtgatgt caacggctat accttttccg gtgccaagat gacgggaacg
     2641 atgacactct tgaaaacagg gaacgcctcg ctgacgttgg agaacgacaa cgactacacc
     2701 ggcctcacca caatcaacgg gggcacgttc cggctaaacg gatcgcttga cggctcgccc
     2761 ctgctcgtgc agccgggcgc ggaactcatg caggggacga acgcttccat ccgcggggat
     2821 gtgaacgtga atctcctcgg acaggcaacg ctctccggat cgaacacctt caccggcgat
     2881 ctggtcttcg gttcgatcgc cgaagggaaa gccacgggaa cggtttgcaa cgtctcggcg
     2941 atcggcggag acggcggtaa tctcgttgtg atgcataacg cgcgagtcat gctggatgcg
     3001 gagggcgtct accctaaccg cacgctccgt ttcgcggccc gtcaaagcgg cgccggaacc
     3061 gtgccctacc tcaagcagat gtccggcaaa accgcgacct ggcccggcgg catcgcattt
     3121 gacgcgccgg cgtatgatta ttccttcgag atttccggaa ccctgaatct cggcaccgat
     3181 ccggacgcgg tgatcacgga cgcggggagc gcttcggctt cgacgctcat ggtccgcggc
     3241 accggacgcc tcaatcagta cgcccgggcg caactcaagg gaggtttcgg aaagacagac
     3301 agtgtgtcct ggcacgtcta cgcctccaac aacgtgcttt cgcagttcaa tatctcagtc
     3361 ggctctgcct ttctgcacgc gccgaacgcg ctttcgacga actgcaccat caagctggga
     3421 caggtatacg ggaacaataa attcaactcc ttgttcgact tcggcggttt cgatctggcg
     3481 atcgccgggc tcgcggtcga tcagggaatc tcgattgagg ggaacaccca ggtgttcaca
     3541 acgtccgaac ctgccacgat cacactgctc ggccccggcg acacgatcct gaaccggacc
     3601 ggcaccgaac tgaagggcgc actcgcgatc cgcaagcgcg gcgcagccac aacgctcttc
     3661 ctcggtcagc cgaactcgaa caccggcgat ttccggctgg aggaaggcta caccgttgcc
     3721 agcaccaacc gcgcgttcgg gtattccacg aacctcttcg tgggagcaac gggcaccctc
     3781 acggtttccg cctccgacgc cctgccgccg gaggatgacg tttggctgcg tgtggccgac
     3841 ggcggcaccg tcgtgctctc caacgacgtc gccgcaacga tctttggcct cgaaatcaac
     3901 gggcggcccc gaggtgcagg cacctacggc ggtgagggaa gcggtgcgcg gattatcctc
     3961 cccgccgtat tcagcggcac gggaacactt tccgtaagaa acggcgcagg cacgatcttg
     4021 cggttacact aacgcggccg gagccccgat caaggcccgc tgcgctttgg cgaccgcgcc
     4081 atccatcagt tcgtcgatca ctttgcccac gtcctcggtg gacattttcg ccgtaatacg
     4141 gatcatttgt ccacatcggt tttgccgaaa atcccaagcg gcaggaatgc ggacgcgcct
     4201 gccgcgtttc acccctctga ttttgtttgc gcgccgagtt tcccggctgg cgtccggatg
     4261 atttcaacca tgttttccaa cgcgaacagg atgatgagca cgcccgcgat cggcatcgag
     4321 acgtaaacca tgccccgctt gatcggcagg gcaggcaact gctgccccat ctgccccatc
     4381 gccaatcccc aaccgccgat tacgaacacg atcagagcca gggcaaggat caccagttgc
     4441 acgacgacgc tcatcgtttt gcgcgcttcg gggtgcagct tggacacgaa gtagtcaaca
     4501 ccgagatgag ccttcgcacc gaacgccagc gcagatccga tcatggagat ccaaaccagc
     4561 agcacacggg ccagttcgtc cgtatagtgc gcctgatgtc ccaagcagaa acgggtgaaa
     4621 actccccaca gcaccgtcag cgtcatgacg gcaaccatcc ccatcagcag gatttccaac
     4681 gtcctgacaa agccatcttt tatcttctgc cacagcccgc tcattccaga atcctccttc
     4741 tttgcaatcc ggcttttcag cggcggcctc acgccccgct tactcgtgcg cccgctgacg
     4801 cagcatcgct gctgcaatcg cgacggcgat ctcggcgtca ggcgaagcgt cacgcttctg
     4861 cgctttttgc gctttcactt tcgcctgctc gtccggcagg atgtgattga accggggaat
     4921 gatcttggaa ctaagcgtca aaatcacaac aagtagtccg agaaaacccg aaaccgtacc
     4981 cacccccaca accatcagaa taagtccctg taacagcatg ctcactcctt tctgccggtt
     5041 atttcgcaag cgcaggcacg agaacgcgga tgatcagcca gacgacagcg caccctgtca
     5101 cgaggaacag cgtgtacctc cgccagtcag ccatggtcgc gggagcgctg ttttctccgt
     5161 tcgcgccatt aaaccggcgg atgtcgatgt agcgggcagc gaccagagac accaacacca
     5221 ataggtaggc gacgtccggg agggagggga atcgtggacg gttctgagat agataggcaa
     5281 gcaagatcaa caatatcgca ttgcctagaa acatccagta aagacgaatg agacaaccgg
     5341 cctcattgct atgctcggga attggctggt tctgcatgac gcaccatcca tttcgtttgc
     5401 gctaaaacgc gcagtccaat ggatggcggc ccgaccatct cgttaacctg cgggggctcg
     5461 ttatcccccc gcacccggat aagacacaac atgaagttgc gaatacatgc actataacag
     5521 attcgtaacc cgaactgcaa gcgcgtattc atcagcgatg cgaaacgcaa ctggaaatcc
     5581 tcacgactcc ccagacgttt gcgacaggcg aggcggtaca gaccgacagc gcacatgaaa
     5641 ggggtgggaa gtgcgggttg ttgacgcaga accccggatc gttagcgata cagggtgcgg
     5701 ttgcgtatca ggcgcgccat ggccgggaac gtttgatcgg gttgtcgagc accgtgttaa
     5761 cgaggttgac gtcctcgacc agttggaaat cgtacatgcc cacgcagatg aagtccgcac
     5821 cgctctggaa ggcgtaagga aaggcatctt tggggtgcag ggcgcctgcg gcgagaatct
     5881 tgaaggcgat ccacggctgt tcgagcttct ccatataggc gatgacatcg tcaggatcca
     5941 gacaccagac gttatccctt gagtaatagc ccgcttccgg gcgggcggac cagtactcgg
     6001 tacggtgcag ggtcttgacc cagaagtcgg ggatgatgcc gttctcgacg cagcctttga
     6061 ccgtttccag acggtgggct ccaataccga ccggcacacc cagtttgcgc atgccctcga
     6121 ccgcctcgcc gatcttgtcg aactggcctt tcaaagcgag attgtctgac cttccgccgt
     6181 ggcagtaaac cgctgacgcg ccgttctcga ccgaaagctc cgcaccgcgg accagaccgt
     6241 ctttatgtcc gctgtcggag atgaactgga ttttgccgcc ctcttttctc cagtagtcgt
     6301 tgatgacacg catcagggcc gggttggtgc tgatggtgtt gactccacag gcctcggcca
     6361 tgcggaatgt gcggaagaca cgctcgtcgg tatggtaggc tttgaccagc ttgtcggtgt
     6421 agagcaggtc gcgggcgtgt gcccagcccc cgatcaggtt gccgccaagt aacatgcgac
     6481 tcagtttcag gttgccgatc tggccgaatt tgtcgaccgg ctttttcagg tccttgatat
     6541 catcgaagct gaaggatttg acggtggcgc tggtgaccgc atcggtggtc ttgaggttct
     6601 cgcgttccca ggctaggccg tgcttgcggc tgaaggcgta gccgaaggca ccgatcaccg
     6661 gcaacgagct gaggctgagc aacagctcgc ggcgtcccgc cgaggacacc cctaaaccgc
     6721 taccaccgtc tgcggcgatg tcgcggcggc gcaggaacaa acaaagacgg ttccataaga
     6781 tgggaccgat ctgatgggaa ggcacgagac agaccgcgag taaggcggcc gcctcgacca
     6841 cattgcggtc gatccataag aaatgccctt ccgaggtata cgccaggaaa ggcggttgtg
     6901 cgatgtaata gaaggccaac agcagcatac ccgcgacagc ggccggccgg accagtacgc
     6961 ccaagatcaa cgcaaggccg atcagggtga gcccccacat gttggctaaa tcgcagagcc
     7021 gcagcaacca cggagtgttg gcgatatgcc ggaagagcgg cgcagcgaac cattggctca
     7081 tgcgcagata gccttcgcag gaccagccgc cgggctggat caacttccag accccctcgt
     7141 acaggaagtg ccatccgatc agcatccgca acagagtcat tccccagttc ttcttcattt
     7201 taccccgttt ggtttgtccg gaccggctac ccaagttcaa aagtgaattc acattgtatc
     7261 cgaacacgca aaacggacaa gcacttatta cgccccgtcc cttggtcgca ataatcagga
     7321 attcagcgaa ttgtcgcgcc cgcgaaagcg gcatgtacat ccgcttcgtt catttgcgtc
     7381 tcccagtaaa tgaggttctc cgccttcagc gtgccgactc cgttagccag cacaagttca
     7441 gatcgatcgg cgatgtccca ataggccacg acacgacggc cctgtcgctc gaagacgaac
     7501 gcacgcacgc gtgaccattt gtctccggcg acgtcaagct gacgccattc gaccagctcg
     7561 tattcgttcc gactgttccg gtacagatga aactccttcg tcgtgtcctt gagaagttcc
     7621 ttctgcccgg gcgtcagcca tttgcgtgcg cgcacgtcct cccaccggcg tatcgtctcg
     7681 agaagatcat ccgttcgccg atgcttcttc aaggtgtcga gcgacaactg catcgtggcc
     7741 ggacaatcga aagccgcggc cttgctcgtc ccatattccc agatatccgg ttgcgtaccc
     7801 accgtgcggt ttccggaccg gaaaagaccc tctttcgcag gaggcggccc gtcgagccgt
     7861 gcgggccagc acccccacca tccgaagtcg acgcgcgtaa agtccttcgc cagccgctgt
     7921 gccgcggcat acgggtactc gatgatcttc ttcttgaaga tctccggacc aaacacgtcg
     7981 aaggcgttcg caccgctctg gaagtgccag ccgaaatggc tcttggcaca cccttgggtg
     8041 aagagcggcg ccctgcctgc gcgcgccgtg caggcgttca cacagcggta ctgcgacagc
     8101 gagatgttga ccgtgcaggg cgggttcgcg tcttccgaac cgtcgaaata gaggaaatcg
     8161 aaaccgcagg cgtagacgcc ggcgattttc tcggccactt cgtcctgcaa ggaagaatcc
     8221 tgatccacgt aggcgctgat cgccgcgcat tcgctcacgc ccagcacgcc gccgatctcc
     8281 ccgagcggat gggccttcgc gtaggtgccg aggtggcggc gtttcacgcc ggtgaatgtg
     8341 taggggcgcg tcgtggtata gcccgtgtac gtcatgagtt ccgtgccgaa cttcaaaatg
     8401 cggattttcg gatgcatcgg cgcggcgacg gggttctcct cgacgaagac ctcgcatggc
     8461 tcttccgaga gcggcagcgg cttcgcaagc gtgaagcgct tgacgagcgc cagccggtgg
     8521 tcggcctcgg gcgtcacgta ggaactcttg aagccgatga acgtctgcaa cgtatgcagc
     8581 cccaccgaaa cgccttcttt gtggaaacga ccgagtgccg ctgtgagctt ttccgcatcc
     8641 cagccattgt cgaaggaata atcgggcagc gtgttgtatg atttggcctt gacgatgccg
     8701 ggaaagtaga cgagcaacat gcggaagccg cctttcttca gataggagag gagcgcgtcg
     8761 acgttctctg gccggaagtc gccggcggtc caatagagcg aggcgttgag gcggctgtcg
     8821 cggcggctct gcacgccccg cggcaggccg agatcgcgct ccacggcgtc gatctggtcc
     8881 aggaagcgtt ccttctgcga ttcgacggcg aggacggcga cgccgttcgt gacgccgtag
     8941 tctttgagcg actcgacggt cagcttgcgg aagccgtgcc ggcgctcgtt gtccacatcc
     9001 atgtacggcg tgccgcccat gacgccgacg aaggcctggt cgtcccacat cacgttcagc
     9061 cagtcaccga agttcgcgcg gttcttcacc ggcaactgca ggagccggaa cgcatcgatc
     9121 ggcggcacgt ccaggttcca gtgatagtac tggtgctcgt ccgtcgtgtt ggagatgagc
     9181 cgctcgggcc tgaatgtcgc atatccggcc gctgtctcgg tcacgcgcac ggcgatctgg
     9241 tagggcgccg tctcgaagcc gatgaagatg agatcaccct cgcgcctgac ccggttcgcc
     9301 gggtacgtcg tgcgcttcgc ctgctgcacg aggcggatct cgttgttgaa gggccggatc
     9361 tgcgtcgacg cgaagaccgg aatcccctcg cgcggctcga gcatctcctc gcccgtcgcc
     9421 ttcacgatca gtgacttggc ggtggcatcc ggattgatga tgagcctgaa cgtcttcgtc
     9481 tcgaccgtga cgggcgtcgt cccgtcaaaa ggcgtcttcg gcatttcctc agcgcacgcg
     9541 gaaaatgcca caatcgctcc aagcgtcaaa tataatctgt ctttcattgt catttcgtcc
     9601 tttacggtta aggtgattcc gacaaaacct ccatcagggc tgcaaacacc ttcggcgacc
     9661 gatccgaacg ggggcggagc gtctcgagcg gccgcttctg aagctcattt cgcaacactt
     9721 cgccagattc gagctggagc gggctcttcc tgtctttgga gcggatgatt gtctcgatct
     9781 tcgccgccca ttcatgcgca cggggcgcat accagtagcg cgccagctcg tactggtggc
     9841 tctgacaata ggtgttggcg gcgttctcga ataaaacgga cggaaaatta ggattgcgca
     9901 ccggctcgat ttcattcaag cgcaaatagg attcccagag cgaataatcc gtatgcaatt
     9961 caagaagatc cgccatacgg tcggcaagac cggcaaggcg gttcgccgcg gcaatcatct
    10021 ttgcgccgtt ttccgcgccg ccttgccatt tctcaaaatc agcggccagc gtaccggcct
    10081 ccatcaccat catgcggtcc agtaccgtac gcgccaaatc aatcgtatcg cgacgaacgg
    10141 cctctccctg ccactcaatc ttggccaact cggcgaacac gcccttcgcc tcagcaagcc
    10201 tcctgcccca cgtttcggcg tccgtttttc cggcgggacc atattgcttc gtcccgaacg
    10261 acttgcgcgt gaacaggtaa ctgtagttgc ttccccagtc actcatgtag gacacaggga
    10321 tgaccttctc ccagagcgat ttcattcggg gcgccaacgc gccataacga ctgttgcaga
    10381 attcgtcaag gacctgccgg tgcgaaacgg acgtggacga ccaggcgttg gccgtaaaga
    10441 atcgcaataa gagcgtgtcc gtatggctgg attccggcca gaagatatac cccacgcaag
    10501 cggagtcatc ctgcacgatt ttctgccgtt cctcgatgag cggatagttc gcgcgtacgt
    10561 cgagcgcatt ctcaaaggcg aggaacaccg agaacgtgta gggaaacttg ccgacgacac
    10621 cccacttcgt gaagttgttg ccgcccagat ccagcatctc cggacgatag tcgcgcgtcg
    10681 aatctccttc atagtcccag atgatatccc gtttcgggtc cagcgaccga accagttcct
    10741 gaacttcctc cggacgccac gtaaagtaga aatcccatcc tgccaaaagg gtcttcgcgt
    10801 tcggatagtc gcgatgcgcc cgtgcgagga acttcttcag cgaagcgatc ttgagttcaa
    10861 gattctcctg acgattggtg tagcaacggc gctcgccaag gccgatcgtg tgtagaagct
    10921 gctgccggtc tttcccttga ccatacgcct caacagcagt ctgggtcagc ttccagtacg
    10981 cgtccaccca cttgtcatca cggacatccc agacgaggcc gtttttctcc gaataaccgg
    11041 ccgttgtctg cggcaggaac ggcggattca tcttctcgag gaactcttcg ggcgtgcgcg
    11101 agtaccaatg cgtcatcgta ccgaagtcct ccggaaccat cagtccgcgt gcgaacgcgt
    11161 attgctgaag gtcgtgccga agcttgcccc ggtactccag cgaccagaag agcgtccggt
    11221 tgtcgtagcc cttaccggtt cccggcaggt tcttggacgc atccggatag gaaacgatgt
    11281 caggaaacgt gcgctggaag agatcgtcct gtccgatgcg gagcatgaag acgttgagtc
    11341 ggcgcttgag acaccagtcg atctcccgct tccagtcatc aagcccccag tgctcggctt
    11401 ggaaacgcgt gagtccccgg tgcgcgaaat aacggagacc gcggtattca aagcgagcct
    11461 cctcatttac gtcgagcccc gtgaggtcga tgtcctcctt gtgcggcacc acatcaatat
    11521 cccagaacca atgacagccg ccgcgacgct ccagcaaatc atacagtcca tagaagaggg
    11581 accggacatt ggatccggta atgacagccc cgcctgtttc ggatctgatc gtataggcgt
    11641 catttcccct tttcgatatg gaacgatcaa tcgcgaaccg aacggactcc ttcgttggtg
    11701 tcctgcctgt tatctgccgg taatatttac cgtactccgc cctcgccaag tcaattccgt
    11761 cggcaaacga tacggaaacg gcgatcatcc ccgacaagat caaaacgccg gccttactca
    11821 aactaaacat aacctatacc cctttccgcc tcgtaaccat ccgccgacat cggcaggcaa
    11881 ctacttcatt ctgattgacg tgccgccggg aacgatcttc acggtcagac tacctccggc
    11941 tgccgacagc acgatgtttt gatagcggcc cggcagtccc gctgtgtcaa ccgaccagtt
    12001 cgagaggtca cattctccaa tacccgtcaa aaggacatac gacccataag tcaacatgtt
    12061 cgtcgccccg gtgaacgtta ccgtaccccc ctttgaaaag tcaatcgtcc cgtcgaccgt
    12121 cacaggcgtc accgtaccat cggcattgac ggcagcctca agatctgcgg ccgattgaag
    12181 ggcgacccca ccgctgatcg tgccacctgc cagcgacagc gtttcgaccg aaagcggcgc
    12241 gttgccaacg accttcagcg tcgccccgtt cgacacggcg accttcgacg cctttgtgcc
    12301 attccaccct ggcgtcggct gaccgaacca tttctgccga agatatgcct caacaagggc
    12361 cacctcacgc gtcgagagct gtttctcata aagcatgtac tctgcaaatt gctcgccgcc
    12421 agcatagcgg gtgctgctcg caacacccgt cagcgccgat ccgccgaagt tctcgtaaga
    12481 cgcccacgag acgatgttga agcccgcaga gagccccgtc tgtcgcggat cgcaggcaac
    12541 tccattcttg tggaaccgca cgccacgctc atccattgcc gtcttaggca taagcatgtc
    12601 gcttcggaca acgggctgat ccctgtcccc ccccagcggc cccgatgtct caccagcacg
    12661 gacgattgtg tataccgatg accagtactg ggagtcaggc gccgacgttc ccaccagatt
    12721 cccgccgcca tgatccgctc cccaaacggc aatgaccgta tgaagttgtg gagtggacgc
    12781 ataagaaagc acgggggccg acggggcgta ctggttggtc ggcaggccac ccttttccca
    12841 tcgctttgcc tcaccaaagt cgacataggg cattccgcca agcgccgtac tcgtccgcag
    12901 ggttggataa ttcgtgcaat agtccggatg cgaggccgga tgttcaccgc ttgcagaatc
    12961 cagccatttc aaaatgtttg tgacgccgtt ctctaccgta tactcgaggt tgttgagatt
    13021 ggacgcatca taacgcgccc acgctccggc gggcaacatg ttctccgttg ccgcaacgga
    13081 ttggatttcg agtgtgcctt ccttcaccaa aaaggaaacc gaaccgtcct cgggcaaggc
    13141 cggcacgagc aaggtgccgt ttcctgtctt ggcaagcgta cccgcaccgg tcacccgatc
    13201 aaccatcagt tctgcgccat ccttgacacc gatgccgccg ctgccctcgc ccaagttcaa
    13261 cgtgcccagc gaagcttcag aaccggtttg aatcggcgca taacagattt ccgtcgtctg
    13321 ccacttgcgc gtcagatagg ccataatctg cttgcactcg acgaccgtca acgcatttgt
    13381 gtaaacgatc aactctccca ggcacttgtg cccgattcga ccttccttgg cccggacagt
    13441 gttgtattcg tacgagtccg ctccaggcaa ttcataattg gcattgcact cgaacgccaa
    13501 aacaatcggg acaaaatact tcaccgaggc attttcatag gcgggatagg gatatccctg
    13561 atccggattg ctgagaacgc cattgatcca gagtttcccc tgcttgatgg catctgttcc
    13621 ccagtcgaaa atcttgccgg tatagacgtt gtaatgagcc caggcttccg cctccgtgcc
    13681 ggtctgttgc gtgtcaatga tgcccaagaa attacccccc gaccgccaac gatcctgcgc
    13741 catcacctga aagacatggc ggatgttctt tacaggttta ctccagcggt gcgtgtcgct
    13801 atccgcagga gatgaactgc cactgacgaa attaccttcg aaatgaacat actggatgtt
    13861 gcgggtcgta tccgtcctca gttcaggaaa atagcccgac cccggtgcgg aacacacttc
    13921 cgaataaatg taatcaccat caggatcgtt tttctggcga acatcctcga tgcgtgtaac
    13981 ccccttcaca ccatttgtga catcaacgag agtcattgcc gaaccgttct tcgagcagtc
    14041 aacccaaaac gccgggttgc cggggaccgt ctcgccgcct tcagaacgga tgatcgatcc
    14101 gtcacactcg aaaacgattt ccgcgccatc ctcaagcgtg agtccgtcta tcgaggccac
    14161 attggaaaag aaaagtttac cagggccctt cacaacacct ccggccagac tgccgacacg
    14221 gttcgtgaca cgaaggcctt gcacaggcag ttgcagaacg gcattcgacg tgatcgagaa
    14281 cgagccgatt tcaatcggac acgactcatc ttcagccgtt ggcacaaacg ttccttcttc
    14341 gataaccagg gcacccgtat gcggcccctc gccgaatccg actcgtaaca aaccggcccc
    14401 ccccttggtc agggatccct tgatcgcctg gaattcatgg atgacggcct ccatgttgcc
    14461 cgtcaccgag aagcgggaat tctgtcccgc atccccttga cgcaccgttg catagggaag
    14521 gccaggacca ttctccgtgt taggagcatc agagaagagg atatcacatg tgccgacctc
    14581 aagcgccgta ttcaggactt tcggtgtgtc atggagaatc aggcggccgg gaaatgtatt
    14641 gttgcaatga atgcgcgtcc agcggttgcc accgcttaaa gtcaacaatt tgagcaatcc
    14701 aacctcaacc tcatatatat cctccatgcg atacttgcca aggtttatgg tgaagttgtg
    14761 gtctgtcgaa ccattatgcg ccagctcatt tacataccgg cggcagtcgc ctttccgtcc
    14821 ggcaaaggaa agctcagcag cggccgccgt ttcggaaacc ggctgcgaaa gttcaacaaa
    14881 gtcatccttg ccgaagatcc gcctgagaaa agtgccgccc gaaactcctg cacccgacac
    14941 cagagcgccg atcggcaggg tatcggccgc accggccgtt acggccttaa caaacggcga
    15001 accggcttgc gtcgagaact tttgagtgat ggccgcggaa ctcccgcgcg gcccgttaaa
    15061 tgacagcgag ccgtgatact cagtgatcgt ccccaaggga gaagggcgcc aggtggtctc
    15121 cgatgacggc gcatactgat tatcgttgat ggaaaacgta ccgccatgga caaggatttt
    15181 cgccccgttg ccaaaggtca tgtcgccggc aagtgaacag ttcctaagat aaggcgtact
    15241 ccccttgatg tgaagcgggg caaagaaggc catgttcttc gagaacaaat tggatacgat
    15301 tgaaaattgg tgatcgctga tgtaacgagt cgttgaaccg gcattctcta cccgtgcgac
    15361 ggtgacgggc atcgtattcg aaacccatcc gccagaaaca gaaaccagag cgtcatcgct
    15421 gttggggatc gacttggggt tccagttgtc ctcatccgcc caatcgggca tggacaccgg
    15481 gcccgacgcg ctcgatccgg tccaaacata gtccgtcgca gcaacagcca tttgcgcaaa
    15541 agcgaccgcg aacatcgcca tgcccccgcg caccgcgccc gcgcttcttc ccttaccacg
    15601 cgtttgagac agtttcatgt tgttgttcct ttgttttggt ttgtcctgac ccgcactcca
    15661 cacaacgaaa cgaccgaact cagcctttac cgacattctt acttcagacc tgctgaagtg
    15721 atctcaaaaa tcgcaaattc acccggtccg agacggcaat cgaacgaaac gccagccgac
    15781 acggcgacaa ctgcttcgct caaaagctca cgcacggcgc agaggcgttt cttatcggac
    15841 ttaaacgtga tccggatatc cgatgtcttt gttgcgtcaa gttcttgcgg agcatcagca
    15901 aacttcgtca cgccgtcgtt gttgaagaca taaagccagg agtgcctgtc agcaactgtc
    15961 aatccgtaca tgcagtcccc ctcgacctca aacgggaaat actcggcctc aaggtcgtta
    16021 agcgccttct cgagtttcgg ataccgtgtc tcgccgtttc cgatcccccg accctttccg
    16081 atcttgaccg gatcggccag catatccaca ccgacatgaa tcaccttgcc gccatccgcc
    16141 tcatatctgg caagcgtttt ttcccaagac gtgtcgggat agtcaccggc gacgacgagc
    16201 gccttgtagc tcttcatgac ggccagcaac tcctctggct tctggctctt tgcatccggc
    16261 gcaatcacat catacatgtt cgcataggga gaattgcgta gacatttctc ttcgccgcga
    16321 tcgcgacaag cggtatagtc acagccgggt acgagcgtaa agaagacggc atcgaccgcc
    16381 ttgtcgcctt cggtgtaacc gaacgaggga tcggcgaacg gcgccccccc cccaggtcgg
    16441 atagccctgc gcgagcggca cacaaatggc cactggagaa taatgggtac cgcgatcggg
    16501 atgatccttt atgaattcgg caaatgcggc atagatctca ccacgcggcg agaagaccgt
    16561 cctgcctttc tctttgtccc acatgttgag gataccgcag ttccactcct ccagttcaat
    16621 atagtttgca ccgctcaggt aggcgagata atgcgtgcgc ttgaagaggg aacgcgaaat
    16681 gccgaagtcc ggcccgctgt ttccgatgcg gatcttgccg cccggacacc acgatgcctc
    16741 ccgctccttc gcataacgcg gctcgcctgt tgcttcgggc gtcgccggat agcggcacat
    16801 cgcatccccg agaaaccccc tgcccttggc cgacgggccg ttggcatagc cggcgatata
    16861 ccattcccac ggtataccga actgccgcgc cgcgccgcgc gtgaacatcg cggagggctg
    16921 gtagcggtac tggcccgaac tcgtcgtctc cattcgcagg agcaccgccc cgaaatcggc
    16981 ggcgatgtgc agcgaattga ggtgcgcgtc aagtacggac atcgttccgc cgtagttcct
    17041 ttccttgcgc atttcataat agcgcttgag caacgcgaca tgctcatagc ggtttttcgg
    17101 tttttcgccg aggaacgccc gcaggatcgc cacgcggttt gacgaatcgg gccgatggtc
    17161 aacgtatgtg cccttctccc ccgccacgga actccatgcg ttattgaggt cgttacacca
    17221 ttcgctgcag gcgccgtccg ccacaagatt cggatgcgct gcccgccagg ccgcatacgc
    17281 cgccttgtcg agctgcacgg gacttcccca cgtcccgcgg ttgcgttcac cgcgtttgga
    17341 gagaaaaaac ggacgatccg gatgcgggtt cgaagtgagg atttcacggt ctggcgcatc
    17401 cggtctgaaa tggagatcca gtgtcggcca gacggcgtat ccggtcgcgg ccgcaggcgg
    17461 ctccgccgct gccacgtact tcggcacgac atcgctgatg gctaagctgt tgttgaactc
    17521 aggattgtcc gcagccagca gtttgccgga ccacccgtag ggagtatatc tcgtcaatac
    17581 gcgccagcag tcgcggccca tgatctcaag cggcttgtgc cgcgccggca cctcgacaag
    17641 cggcacgcca cacacatccc gctcatcgca acctgtcaat acaaccaacc ctaaccccgc
    17701 cgcgagcaaa ccgcgcggaa acacatagcg gacctgtgca tgtttcatgc tgttttcctc
    17761 cattgattaa gaatcggacg gcagaatttc ttcttgcctt tttcacaact tgagtttgct
    17821 tactcatcgg attatgacag cgagccccgt ctggctggac accttcgcga tcaacgcacc
    17881 gacgtccatc atgtctccag cgctctttgc gtactcaaac gacccgtccc cgtccggcac
    17941 accgagaggc tatccttggc cgtagcgaca gccgcctgaa tcgttctaaa cgccgtctgc
    18001 tcgctcgttc ccgatccgga atcgtccggt cgcctccagt cgacaaagta ttccgtcgcg
    18061 caggcagcgg tcgccagaag tgacaaaaca caggcaaaca gtggagattg cctgcaaacg
    18121 gcacattggt catgtctgtt tgtcatgttg actccttttt catttgcgtt catttcagcc
    18181 gcaggacgac gataccggca ggcgggatct tcgcgacgag tgacgtaaga tcttccgcct
    18241 tcgccgagat gacgatgggc agtgatggcg attcggtcag ggcctcggcc gtcgtaacgg
    18301 gaccgcgaac gagaatattg gcgttcaggt aggtcggccg gtcattgacc gcgaagacga
    18361 tgcgcgaacc atcttccgcg acataggatg aaagacgcaa atcagccatg tcagctccgg
    18421 ggaagaccgg agaagctcgg ttgacctttt cggcggccgc ggcaagggcc tgagggtcga
    18481 acgcccgaaa cggaagcgga tgacgccagg acgccgcatc gaggacggac ggccgcggac
    18541 cgaacggaca ggcgggagaa ccgtaaccga aggtcacgac tgccgcggag cggtttgtga
    18601 gagacgcgag ctcgtcagcc ggaaagcgac cggaattcag gatgaggagc gggaggtccg
    18661 catcggcctt cgcctcttca accgtaacag ccgaattgac cgccgcgcca gcatttacga
    18721 gatgggcgag aagggtgtac gcgctcggat agcccccgct gcaataggca tcgaattctg
    18781 cgtccatcgc atgtgacgag acgaccacac gtactccgtc cgcccgtttc ggacacttcg
    18841 caaggttcca agccgtgtcg aggcgacgcc actcctcacg cgtaaagccg tccgtcaggc
    18901 accacaggac atccggcacc atcaaacggt ctccccgcag gagatttccc agcaaaacaa
    18961 cttcagccgt cacgcgctgc ggcgcatcgc ggagcgaatt gtactgttcc atgccatcct
    19021 tcacgcacgc aagatggatg atgggcgtat cgacggtggc acccgtcagc aagaaggcta
    19081 cacgacagct atcgagcaac gggacaggcg aaaaccgcca gccctcgagt tcgagaacga
    19141 tcgccgaagc ctcagccatg aaaccgtcca accccgcctt ctccatgagc cggtagtcga
    19201 taccatgccg atacagcgcc tcaagcggat cgcgcgtcca agacgtgttc gcatagagct
    19261 tgagccccct cgcatgcaga gccgcggcga gtttagcaat gaatgcagca tgtcttgcgg
    19321 catgcgcccg gcagagggac gcacggtctt tggcctcggg gaagaggtgc gaatagtcaa
    19381 gctggttcag cggataacgc ggatggccaa acccgtcgca ggcatgcaag ccggcgaatc
    19441 cgtaatctgt caagaactgc acggcctgtg ccacaaagaa gtcagcatag tcccttccgt
    19501 ccgccagccg ctcgatcggg tttacattgt tcgcccgcct cccgtccttc atccgatagc
    19561 cgacctctgg atgacggtcg aaccaggtca cgtccttacg cgtcgccttg aactgacgga
    19621 gccaggagtt ccccttccct ggcgccatgt cgaaaaatga aggatacgag gcgataccgt
    19681 acttcttgag ttcggcgata agtccgcgga attgccaagc cgtccacttc tgccgcagac
    19741 gctcctcgtt ccacggacgc gcaaaatacg aacagtggag gtcgccgatc tggaaatctg
    19801 acgagagatc cgtatgcgcg tgaaaaagct caggatcgaa gataagaaac gaaaccccct
    19861 tgggcttgac ggacatcttg tcaagatatg cctgaacccc atagtcgggc tccgtattat
    19921 caaaagcgat gagttccgtc caaagatact tgtccgcccc gcatgtacac atggccattc
    19981 cgacaagcac taccagtgcc agcagtgctg atcttcggac actcctactg tggtctaaca
    20041 taataatatt ttccttccag gttcttgaac ggcagggttg caaagtcgtt ggtcgccgtc
    20101 cccggcgtca tgcagaagat ctggctcgca gcgagttccg ccgcgcccgg ccagagatag
    20161 ccctgcttga cgacgaccgc gtcatagtct gcggcggcaa cgtccatcgc cgcgaaatgc
    20221 tctttcatcg tgaaagacgt cctgacagag gcgaagacga tgtcgacatg acccgctcgg
    20281 aagagaatgc cttctccgga ctcttcgcct acgaagccga ggatcttggc cccgtgaatg
    20341 tattcgccct cgatcatgag gtcgtcgtta tactcatccg acttcgggat cgtcaacgtc
    20401 gcgcgatcgc ccggcttcat ctctgtgagc ttggcccaag ctgtcggaca atagagcgag
    20461 gtaaagagtg tctttacatc gctgccgagg aaacgcttca gaagaaagac actctcgccg
    20521 cccgcgccgg ccgtcgtgtt gtcgcccgag tcgctgacga ataccggctt tgccatcgtc
    20581 ttgacggcat cgaccgtcct gtcgggcgcc agtgccggca cgccgaactt gagatccgtc
    20641 ttgtgtgcat cgaaatagtc cgcgatcgct ttcgcctttg cgtgcacggc ctcgtgattc
    20701 gcctttgagt aaacgatgac cgaggccgac atgcacggcg cgtccaccca cggctggccg
    20761 ttgaagaccg aagccgagat gatatccgga tcggtatcga gctcgcgcac catcttcatg
    20821 atcgagacat acggctcgac cgaggtctgc gcggcatccg cgaactgaac cttgatgcgg
    20881 aagaaatggg ggaccgtcac cgccttctcg ttcaggatgc gcatgagcgc aaccgccgcg
    20941 cgcgtctccg tctcgtcgta gtccgtgtgc ggtgccgtgc ggtagccgct gatcgcctgc
    21001 acgttcggaa tgagcccgtc cgcgaggttc ccgtgaaaat cctgcgcaag cgagatcggg
    21061 atctggtcgc cgatcgtctt gcggagttcc ttgaccgtga actcctcgcc gctccccagc
    21121 ccctcgacgc acatcgcacc gtggagataa aggtagatcc ctgcggcatc ggaatgcttc
    21181 gccgcttcct tgcggaagtc ggcgagcacc tcgtcgaagc actcccgcgt caccatgccc
    21241 gacggcaggg aaacggcgta gacgagcgga acgacctcga acccggcctc ttcgaagaga
    21301 tgggacgcga agagtttgtt cttcgcctcc tctccgtgga ggatgtagaa gttctccttc
    21361 cccgccttcg ggggcgagaa ggtgtttgat tcgcactgga atgatccgac gaggattttc
    21421 attggctttt cacaattagt aagttgagcg tcggaagttt aacgcagaga cgcagagatt
    21481 tttacgggat ggcggcgtga cctccgccgg gggagggacg agcgtctgct cgtccgcggg
    21541 caagcagacg cttgcccctc ccgatgctct gcaatgaacc ctgaaaccaa gagaagtgta
    21601 cgcatcagcc tttcgcaaga tacgctgttg cgggcgggga cgcccgcgct cccgactctc
    21661 tgctctcctt ttctccagaa acgcatgatc ttcgcacttt ttcgaaatcg ctcaaagaga
    21721 acgtgaaaag tacgcgtcca tcttcgggga catcggcttg gagccgtttt ccgtgatgat
    21781 gtagccggtc tcgatgcgcg cgccgttgaa cttcggatgc ccgaagaagc tga
//
LOCUS       DAOSRB010000022        21804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2594_length_21804_cov_7.949193, whole genome shotgun sequence.
ACCESSION   DAOSRB010000022 DAOSRB010000000
VERSION     DAOSRB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21804
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2594_length_21804_cov_7.949193"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PLZ74_02365"
     CDS             <1..928
                     /locus_tag="PLZ74_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97197.1"
                     /translation="GHNRRVVYADGTAVMYGKADRPDFRGLEGWEDHAVDVLCFTDSE
                     KRLIAAALAVPCPSQTVEGLHEVSADYWHHVRERLKAKYGEGLVVLGFCGPAGDQVPR
                     PMLRGAAEARMERLRKLSRKQELGRRIAAAFDDTWEVIRSDLRTDVPFAHRVERFQVP
                     GRKLTDQEYEEAKKGYESYAKRDEKEPAVYSRKRWYKRTLDRYEAQQKSDPVNTLEIH
                     VLRVGDLALATNPFELFADYAMRMHGRSPAGQTMLVQLASPADVTHSYLPSERAVKGG
                     GYSAVPQSTPVGPEGGQILVERTLDTIRALFP"
     gene            1138..1482
                     /locus_tag="PLZ74_02370"
     CDS             1138..1482
                     /locus_tag="PLZ74_02370"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOR97198.1"
                     /translation="MAGTTYEMTGSIKTILEPMTFASGFTKREFILTMEDDYPQDVKF
                     ACVKDRTALLEHVAVGERVKVGFRIRSREYKERYYTDLEAFRIDKLEMDGSSVSYEDA
                     EPLTPFDDPTPF"
     gene            1571..2440
                     /gene="prmC"
                     /locus_tag="PLZ74_02375"
     CDS             1571..2440
                     /gene="prmC"
                     /locus_tag="PLZ74_02375"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOR97199.1"
                     /translation="MAEEQKKLRELIALGDAYLAKRNVPDSRVACELLAARLFRCARL
                     DLYRHLDTIPETRLVDALRRGLQRVAGGEPVQYVLGQWAFRNLTLKVDRRALIPRPET
                     EQLVGLVLRSPQVRDTVKPLVVDVGTGTGCIILSLAQELGRGVFVGLDICADALALAQ
                     ENAELTGLAGRVLFAQSDGCGEFDPASVDVLVSNPPYIPSHIVDGLEPHIRDHEPRLA
                     LDGGPDGLTFFRALLLDAVMVLKPGGAVFFEIGDEQGPALRALLDEYGFTEVAIMQDD
                     SGRDRYAMGVQSV"
     gene            3449..3991
                     /locus_tag="PLZ74_02380"
     CDS             3449..3991
                     /locus_tag="PLZ74_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR97200.1"
                     /translation="MNERRLACLSVVLANPGLSVGEIARMAEIPENQASINLRALQAR
                     GLLAARRDGRWIRYDAEADPLVEHAAAVLQALRLEWRRCGDTGQVMETLRAFTHSRRL
                     TLLGFLVRKGGATHEEIAAGTRISRQAVGRHLETLRRTGLVVPTKGGVWKVMARRRLS
                     GLKRDLLDVVENRCPHAHFS"
     gene            4225..5067
                     /gene="nfo"
                     /locus_tag="PLZ74_02385"
     CDS             4225..5067
                     /gene="nfo"
                     /locus_tag="PLZ74_02385"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOR97201.1"
                     /translation="MYFIGPHVSIGGGVANAPKNAKALGATGFGMFVKNQRQWTAAPY
                     AAADIEAFRRQMAADGYTAAQVMPHAGYLINLANPDETAHAKSMTALLDELRRCMALG
                     LDKLNLHPGSHLRLITPQAACERVAQSINRALNQTEGVTLVIENTAGSGGNLGAVFEE
                     IRTIIEGVDDKSRVGVCLDTMHAFAAGFDIRTRDGFLKTMEHFDQTVGMAYLRGLHLN
                     DSKVALGSRVDRHASLGAGLLGLDVFSCILTDARFENMPLVLETPDETIWEQEIRQLM
                     DMAG"
     gene            complement(5118..5882)
                     /gene="radC"
                     /locus_tag="PLZ74_02390"
     CDS             complement(5118..5882)
                     /gene="radC"
                     /locus_tag="PLZ74_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOR97202.1"
                     /translation="MEQPPCPSDQRTVYPDAAPPMRDLPVEMRPREELQRRGAENLPD
                     ELLLAILLRSGLPGKNVTELAREILLRYGGLAALSKTTFEELLHNRIKGLGKVKALEL
                     SAALELGRRAAQQGPRHDTPVIRDPEAAYRVLLPLTRTLQQEIFWTLLLDTKSRLIGQ
                     PIETSRGLLDSSPVHPREVFNKAVRYSAASMILAHNHPSGDPIPSKEDIEVTRRLVEA
                     ARILGIRVVDHLIVGKPSAVSPGYVSLRERNLVAFE"
     gene            complement(5857..6804)
                     /gene="miaA"
                     /locus_tag="PLZ74_02395"
     CDS             complement(5857..6804)
                     /gene="miaA"
                     /locus_tag="PLZ74_02395"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOR97203.1"
                     /translation="MTLAWTLAGATATGKSAAAQLLAERLEIAILSADAMLVYRGMDI
                     GTAKPSAAERARVTYFGVDLVTPDQPFSTGAWLREAARAVAHTNAADSDGKAVPSLLV
                     VGGTGLYFKALTRGLDSAMADPARRAHWQARLETEGVGALREALAALDPQALASLSDP
                     SNPRRLIRAIEQLESLRSLPQNWKQPAPDAPLTVLRMPREQLHRRIRTRVEAMFAQGL
                     VEEVRSLRRHYPVWSASAAQAIGYAESCALLDGTLSQAEAFERIVIRTRQLAKRQETW
                     FRHQTQAVWIDTDERDPPERIAERILKAWRQHGATPLPF"
     gene            complement(6812..7300)
                     /gene="lspA"
                     /locus_tag="PLZ74_02400"
     CDS             complement(6812..7300)
                     /gene="lspA"
                     /locus_tag="PLZ74_02400"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOR97204.1"
                     /translation="MRVLLLAALLVAADQISKYWAVVRLQAVRSVSVIPGLFSFSYVE
                     NQGAAWGMLAGRQAFLIGFSIATLIFMIWRRRALFGALRGRTVIMSLLFGGVVGNLID
                     RLRLGHVIDFLDFHWGDAHFPAFNVADSAICCGVFLYLIMQWRHDRHMARDQMTEPET
                     GD"
     gene            complement(7310..8056)
                     /locus_tag="PLZ74_02405"
     CDS             complement(7310..8056)
                     /locus_tag="PLZ74_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOR97205.1"
                     /translation="MATTRKTKTGSTKKSPPKPVQKATAKRAAPAKRAAPAKRATAAK
                     RAAPAKRAAPAKRATAAIAKKPAARKPAAQAKPARTAKTPPITQSRTVVRKSAVKRGT
                     STEAPVKKGPPSYKLVAKKPVRGSPHFSKRDLNAFKRDLLSMRERITGQSGAMRHAAL
                     QRTDETNPEEDGTNAFMRLQTLEQVSSQLQTITNIDEALRSIEKGNYGVCDTCGELIS
                     KLRLAVLPFAKNCIRCQSEMEKQFRFRGRR"
     gene            complement(8195..10336)
                     /locus_tag="PLZ74_02410"
     CDS             complement(8195..10336)
                     /locus_tag="PLZ74_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOR97206.1"
                     /translation="MMDAAMTFEELETRFFSLLEIRPLPVTDLLPLLQAITTTTDAGK
                     AEEWTMLLIEELVDATDFRGLFLTVMDQVEPLSCALAPDAILRLFKQTCPDRLTDVML
                     DVAQFGEKPLYEALKRLGTLLALEPGTQVIDPAWGFGVIQEADSFYKRFIVDFTRKPR
                     HEIPFATACDSLVPAPQDHLLTVRHNDPGGFAQIVREQPGELLRLTLRSYGDLTAVRI
                     EDLLVQEKILAPDEWKGFWDAARKALKQDPLVVFPAKKSEPLRLLTEAESYGDAWFAT
                     FSATKNPERLLTQAIELETAGRIENLDAAQRAILEERLHFALGAAHQSDAALYARLAV
                     MLSRFGFLTPPTDQLRAHLWEEHRFLRASEALTVKDTADLATFLLAEGSAAAQRLLDA
                     LPQMPFSLLNEVLNRLNTSAEAEEACRRLLTQPKAPPTLVYWAFRNRDTPGWRLPPLH
                     ELLDHAIQIVESRLSGEALRMQNQLKRLFGQTSWLESVFAELDMPQRQRIFERIQASP
                     AWDPATHRSLLGRMLQLDPSLTERKKAAPAPTRESVRWTSWRSLAERQFQYKKLIEVD
                     LLKNSRDIAIARSYGDLRENFEYQAAKDFQRQLLQRQAEIQQELQLVKGTDFADAPHE
                     TAGPGTRVALCLADNTHIVYTILGEWDRDEALNIISSRSRLALCLTGRTAGDSVRIPG
                     PSGEETARIEAVLPLDEPIREWIRTPPPAMS"
     gene            10858..14583
                     /locus_tag="PLZ74_02415"
     CDS             10858..14583
                     /locus_tag="PLZ74_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HOR97207.1"
                     /translation="MRIIAGAVGLMVATGHLCARVESPVVSLDKPLPSEALALTCPAS
                     GTSEPETSAPGAFRTANLSAADSQSLWAALSEARREIGAVGDAADGALLRAHNPGQRL
                     AVRFMPNAVSVAPSGTNAAWQAFFALDGTAGQPAAVVCRGTRMEYDRGSLIEWYDNQA
                     GGVEHGFILKTRPAGAGPDVRLRVAVGGLQPRLSSDGDAAELADGQGNVLLRYAKLKA
                     WDADGRVLASRMEVGDGAVVLVVADAGARYPVTIDPFITAQQAKLGPEVTGDGAAEDC
                     FGYSVAISGDTALVGAYQDDDKGANSGSAYVFTRDGTTWSRQAKLTADDGTALDYFGY
                     SVALAGDTALVGAYQDDDKGDNSGSAYVFTRSGTTWTRQAKLTANDGSDSDWFGYSVA
                     ISGDTALVGAYGDDDKVSNSGSAYVFTRSGSVWTQQAKLTAADGAAGDCFGYSVALSG
                     DTALVGAYQDDDKGSNSGSAYVFTRDGTTWSQQAKLIADDGEVGDWFGTSVALHGDTA
                     LVGAYRDDDDGNDSGSAYVFTRSDTNWIQQAKLTANDGAEMDYFGYSVALAGDTALVG
                     AYADDDKGSISGSAYVFTRSGSTWTQQAKLTAADGAAGDCFGWSVALSGDTALVGAYA
                     DDDNGVNSGSAFVFTRDGTTWNQQAKLTAGESAAGDNFGTSVALSGDTVLIGAYKDDD
                     KGTDSGSAYVFTRDGTNWIQQAKLTADDGATYDYFGYSVALSGDTALVGAYKDDDKGS
                     DSGSAYVFARDGTTWTQQAKLIANDGTASDWFGTSVALHGDTALVGAYGDDDKGDNSG
                     SAYVFTRNGTTWIRQAKLTAADGAAGDCFGCSVALSGDTALVGAYGDDDKGDNSGSAY
                     VFTRDGTNWIQQAKLTASDGANYSYFGSAVALSDDTALVGAYMAVNAAGSAYVFTRDG
                     TNWPQQAKLTAADGAAYDYFGRSVALSGGTALVGADGDDDKGYDSGSAYVFTRDGTTW
                     TQRAKLIADDGAKNDCFGWSVALSDDTALVGAYQDDGLDALGGAAADQGSAYVFTLVD
                     DITVTLDYQYGGITNTLILQTSGTYASLPSATRPLHTFLGWYRTPTDTLEDIQNGVDN
                     HIYPTNTVKRALATLYARWSPFTATRTTPISIRVTALAPFYPGDISQAFPDEVEAYAS
                     GNAAAAAYKVWELILLGLNPTDPDVVPTAILAYITFDGSGNAVVHKIPAVGIPSATIY
                     KQWGKQNLADPVWTDLGTQGTPCPPSQPYRFFKITGE"
     gene            15323..21682
                     /locus_tag="PLZ74_02420"
     CDS             15323..21682
                     /locus_tag="PLZ74_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97208.1"
                     /translation="MKHHVMVVCGLAAAVCGQAREIGFIETFSLAANREQALQELVPG
                     TDEYYYYHALHAQNTGATERFREVMARWQRERDGAVTDSGRELLNRQALLDYDKEPQR
                     SLDYLKRELDLHFTHARKTGERTSGAPSVFDNARIAPEALLKRALAAKPRSLDRVEDA
                     GLPFAAAAKLNDEQRRSLLARLRRPDYPGLTALIAADLKARDSRGFGSLPIHRLLTIE
                     QLDELVRKENGLRNATAFVNAYLAKLAPEDEVDLDTDTAAREAYYERVWNVVRQLDPV
                     HNSLKANVLYNRLRHDQRKGVYDRDRFLEYVKLPRDVHYLRPEIRQQLPRGDYLARLG
                     QDFRLVSLPPIVNEEPLVRRYLLRFFREDADIEAYRPFLRDDFLKPLFAEAKIVHGIG
                     DPQQWVKLLTPDAYKRLKERVDIDFAEENPNEIGADEAVKLTAFVKNVPSLIVKVYEI
                     NTFNYYRETGRPLDLALNLDGLVASFSRRVEVPEPAERRVARVFDLPELKGRGAYVVE
                     LIGNGKSSRALVQKGRLRVLQTVTAAGHAFTVLDEAGRRVANACGWLGGREFKAGPEG
                     RILVPFSTAPKSENLVIRQGDFATLVRFEHLAEQYDLAAGIYVDRESLLRREKAQIAI
                     RPVLRVNGRPASLKLLEAPVLTVRSTDLQGIVTEKEFPDVTLREDAETVQEILVPENT
                     VELRMTLKAQIRNLSLNQKQTLSDDAVFTLNGIERQQTVQDLHAGRTSAGYFVELRGK
                     NGEPLAGEPLACFFKHRWFRDELPVELQTDAQGRALLGALDGIASFRIHTASRTKHTW
                     KPERGACAYPEALHGKAGETLRVPIIQGAADAVSEASLLEMRNGQFVKDWRGALAAAN
                     GFLELRGLPAGDYSLMLTAGGREIAVRLTQGDERDDFVISPRRALERPRLQPLHVTGV
                     ASGAENVEITLANVTPFTRIHLFASRYLPAYDVFARLGFPGGAGLLRQSWQSVRTFYE
                     SGRDIGDEYRYIIERQQAEKFPGNMLERPGLLLNPWAVRETEAQAEKLEAGRAYAGQS
                     AAIMQADGTARHSQAAVVEPPEGYAALDFLKQPAVTLLNLVPDSTGRVTVPRAALKDL
                     PFLRVMAVDPLSTVLVCAALEDTPVALRELRLVAGLDPQKSYAEQKRVTAVAAKESVT
                     IEDVTTARFERYDTVAKVYRLLATLNPDATFGEFAFVANWPKLESGEQRRLYSKYACH
                     ELNFFLYHKDPRFFEAVVAPYLKNKKDKTFLDRWLLGDALEAFLEPWRFARLNAVERI
                     LLGQRLRGQRASVARDTRERAALLTPDMEAFNRRFDTAVQSGALEAEKDGASGTVEAI
                     RKEQADKQAVGFGKQVGAPAAAALVSSPVPSAPEPMLEAADAFRKAEPDTRRAEAKKK
                     EASGMPRQRALPEASPQGRAVDDPFAPAEKEEAESLFFDAGEQDRAASRRFFRKLDKT
                     WELAENNYYRLPIERQVADLVKVNAFWNDYAAHDGRTPFLSGALPEATSNFTEMMLAL
                     AVINVPFQAAAHNEKVEGLQYVLQAGSPLLLFHREIRETPRTGAPGNVLVAQHFFRAD
                     DRGRFENNDRFDKWVTDEFLPGVIYGAQVVMTNPTGNRQKLQVLLQIPVGALPVANGF
                     YTRGAYVVLEPYSTQMKEYFFSFPSTGTFPHYPVTFASNDQVVGGTEPFRFNVVSRLS
                     KADTTSWAWISQNGTGAEVTAFLNAANLHRVELEEIAWRMKEHAFFKSVTGLLRARHI
                     YHDTLWSYGILHNEPEAIREYLRHTPFANRCGLWLASPLLTLDPVERFDYRHLEYAPL
                     VNPRAHRVGPKRTIRNAAFREQYQRLLKVLSFKPELDASDTLAVTAYLVLQDRVAEAL
                     DWFGRVDRKAVPEQLQCDYLEAYLAFYKGDIELARNLATKHAGEGADRWRDRFALILK
                     QIDEIAGGTADMADTESRDPVQDALAATEPMLELHVEAGRIRLDTRNLTACTLNFYPM
                     DIELLFSRNPFLQEGTAQFSHIRPVLSRTVALPAGQNGLTVDLPPEFAGKNVLVEALA
                     GGARRTQAYYANTLKVRKIESYGQLVVTQTATGKPVPGAYVKVYARQPGGEVKFFKDG
                     YTDLRGRFDYVSLNTREAETAERFAVLVLSDAFGAIVFEAPPPKQ"
BASE COUNT         4138 a         6324 c         7147 g         4195 t
ORIGIN      
        1 tgggcacaac cggcgcgtgg tgtatgccga cggcacggcg gtgatgtacg gcaaggccga
       61 caggccggac ttccgcgggc ttgaaggctg ggaggaccac gctgtggatg tgctgtgctt
      121 cacggattcc gagaagcggt tgatcgcggc ggcgctggcg gtgccgtgtc cctcccaaac
      181 ggtggagggc ttgcacgagg tgagcgccga ctattggcac cacgttcgtg aacgcctgaa
      241 ggcgaagtac ggcgaggggc tggtggtctt gggcttttgc gggcctgcgg gtgaccaagt
      301 gccgcgtccc atgctgcgcg gcgcggccga ggcgcgcatg gagcgtttgc gcaaactgtc
      361 gcgcaagcaa gaactgggac gccggatcgc ggcggcgttc gacgacacat gggaggtgat
      421 ccgctccgat ctgcgtacgg atgtaccgtt tgcccaccgc gtggagcgtt tccaagtgcc
      481 cggacgcaag ctgaccgacc aagagtacga agaggcaaaa aagggatatg agtcctacgc
      541 caagcgtgac gagaaagagc cagcggtgta tagccgcaag cgttggtaca agcggacgtt
      601 ggatcggtat gaggcgcagc agaagagcga tccggtcaac acacttgaaa tccacgttct
      661 gcgcgtgggt gatctggcgc tggcgaccaa tccgttcgaa ctgttcgcgg attatgcgat
      721 gcgcatgcac gggcgcagtc ctgcgggcca aacgatgctg gtccaactgg ccagtccggc
      781 ggatgtgact cactcctatc tgccttccga gcgggcggtc aaaggcggag gatacagcgc
      841 tgtgccgcag tccacgccgg tcggccccga aggcgggcag attctggtgg aacggactct
      901 ggatacgatc cgtgcgctgt ttccctgaaa ccggagcccc gaaatgatgg cgtgagccgc
      961 ttccggtgtt gcccgcaacg ctccggtttg gtttaatggc cgcaccgtaa gaaaacggca
     1021 ggccaaccat ccggggtggg cgaaaacgag agggcaacgg gaacgttcaa tgttaagaaa
     1081 agtcaggaat caacaaggtt tctgattgaa ggtatagaca agcgaggact ttgggatatg
     1141 gctggaacga cctatgagat gacggggagc atcaagacga ttttggagcc gatgaccttt
     1201 gcgagtggtt tcacgaaacg cgaattcatt ctcaccatgg aagatgacta tccgcaggat
     1261 gtcaaattcg cctgtgtgaa agatcggacg gccctgttgg aacatgtggc ggtgggagag
     1321 cgtgtcaagg tggggttccg gattcggagt cgggagtaca aggagagata ctatacggat
     1381 ctggaggcct tccgcatcga caaattggag atggacgggt cttccgtgtc ctatgaggac
     1441 gcggagccgc tgacgccgtt cgatgacccg acgccgttct gattgccggt cggtgatacg
     1501 gaccttcctt ttcaagacgg gcaaggcctg gaggattgat tcctgccccg accatgcgga
     1561 tgcggaacga atggccgaag aacaaaaaaa actccgtgag ttaattgcgt tgggtgacgc
     1621 ctatctcgct aagcggaacg ttcctgattc ccgcgtggcc tgcgaactgt tggcggcacg
     1681 gcttttccgg tgtgcccgtc tggatttgta ccgccatctc gacacgattc cggaaacgcg
     1741 tctagtggat gcgttacggc gcggtctgca acgtgtggct ggaggggaac cggtacagta
     1801 tgtgctgggc caatgggcct tccgtaacct gacgctcaaa gtggatcggc gggcactgat
     1861 cccgcggccc gaaacggagc aactggtcgg tctggtttta cgttccccgc aggtgcggga
     1921 cacggtgaag ccgttggtgg tggatgtcgg caccggcacg gggtgcatca ttctgagttt
     1981 ggcgcaggag ctggggcgtg gggtttttgt ggggctggac atctgcgcgg atgcgctggc
     2041 cttggcgcag gagaatgcag agttgaccgg gttggccgga cgggtgttgt tcgcgcagag
     2101 tgacggttgc ggcgaattcg atcccgcaag cgtggatgtg ctggtctcga atccccccta
     2161 cattccttcg cacatcgtgg acggtctcga accgcacatc cgcgatcatg agcctcgtct
     2221 ggcgcttgat ggcgggccgg atgggctgac atttttccgc gcgctgctgc ttgatgcagt
     2281 gatggtgctg aaacccgggg gcgcggtttt ttttgaaatc ggggatgagc agggccctgc
     2341 gttacgtgcg ttgctcgacg agtatggttt tacggaagtg gccattatgc aagatgattc
     2401 gggtcgggat cggtatgcca tgggcgtgca gagcgtgtga gcggtatcgt gtccggggct
     2461 gaccggttgg gtttgccttg gagcggcaga gaacaagagt gcccggaacg ctgaacgtga
     2521 cctgtgacgt tcagatttcg ggcttcaagt ttcatccgca gattacgtag atggacgcag
     2581 gtttttgggg ggcgagagtc tccacgagtt acctagtcca aacacttcgc ggatcacgac
     2641 gaaagccgat gtttgcgagg ctttgttcga attctgcccg ttcaactgcc ggttataggg
     2701 taaccggagt aagggaaaaa gaggtggggt ctgtccacat ctcatgataa gtgggcagac
     2761 cggcgccctg cggaacggaa gaaacgaacg ggccgggcaa accgtgaagg gcggccgttg
     2821 aagtgagtgt tgcttatccg tgtgaagaca gtgatcccgg tcgcgatggg gacggcggac
     2881 ggacatcggg tttgacgccc tagggaacgg gtggtgtgtt gtccacatct catgatacga
     2941 atccggccac aaacagcatt gcgctttgaa caccaagatg tccctgcttt cttgggtttc
     3001 atggtttcaa ggtttcatct aaatcgggat cgggatcgct atcgggttcg gctgtttcac
     3061 cacgggacac tttcttgggt ttcagggttt ctggtttcag ggtttcaagg tttctgggtt
     3121 tcaggtttca aggtttcatc caaatcggta tcgctatcgg gttcggtgtc gctatcgggt
     3181 tcgaaatcga ctgttgcgcc cacgcagtgg cttgcagcga caagtgtaat gacggcggcg
     3241 attccgatcc cgatggcgat accgatggcg ccgcaatgga accctgaaac gcatgagacc
     3301 ttcgatcgca cccctgtcac gaaccgatta cacggcgggc ggacatcggg tttgacgtcc
     3361 tgaagagcgg gtggtatgtt gtccacatct catgagaagt ggacaggcag tttatcatcc
     3421 gacgttgtgg cgtacctgcc gtgcgctgat gaatgaacgg cgtttggcat gtttatcggt
     3481 cgtgttggcg aatccgggtt tgtctgtcgg ggaaattgcc cggatggcgg agattcccga
     3541 gaatcaggcc agcattaatc tgcgtgcgct tcaggcccgg ggattacttg cggcccgacg
     3601 cgacgggaga tggatccggt atgatgccga ggcagacccg ctggtcgaac atgcggccgc
     3661 agttttgcaa gccctccgcc tcgaatggag aagatgcggc gatacgggac aagtgatgga
     3721 gacattgcgg gcgttcaccc attcccggcg cttgaccctg ctcggttttc ttgtcaggaa
     3781 aggtggcgcg acacacgaag agatcgcagc cgggacccgc atcagccgac aggcggtcgg
     3841 tcgccatttg gaaacattgc gccgtacggg acttgtcgtc ccgacgaagg gcggtgtgtg
     3901 gaaggtgatg gcccggcgaa ggctgtccgg cctgaaacga gatcttcttg acgttgtgga
     3961 gaacagatgc ccccatgccc acttctcatg agatgtgggc aagggggggg tacgcgttag
     4021 ccattagctg ttgcaacacc tttaacccgc tgaacgtcaa caagtaagct ttttcgattc
     4081 aacgaaaacc cccacaggca aacgttttcg gtggatcggg aaagcgtaac atcctctgaa
     4141 ctaaaacgtt gaaagtgttg caatagccgg tagcctgtgc gataaaaaag ccgtaagatg
     4201 attgtcgagc cgaagggagg gatgatgtat ttcatcgggc cgcatgtgtc gatcgggggc
     4261 ggggtggcga atgcgccgaa aaacgcgaaa gcgctgggcg cgaccggttt cggcatgttc
     4321 gtcaaaaacc agcggcagtg gacggccgcg ccgtatgccg cagcggatat cgaggcgttt
     4381 cggcggcaga tggccgcaga cggctacacg gcggcgcagg tgatgccgca cgccggatat
     4441 ctcatcaacc ttgcgaatcc ggatgagacg gcccatgcga aatccatgac tgcgctattg
     4501 gatgagttgc gccgttgcat ggcgctcgga cttgataaac tcaaccttca cccgggcagc
     4561 catctgcgtc tgatcacgcc tcaggcggcc tgcgaacgcg tggcacagtc aatcaaccgg
     4621 gcgctgaatc agacggaggg cgtgaccctc gtgatcgaga atacggcggg gagcggcggg
     4681 aatctcgggg cggtgttcga ggagatccgc acgataatcg agggggttga cgacaaatcc
     4741 cgcgtgggcg tctgccttga caccatgcat gcgttcgcgg ccggattcga tatccgcacg
     4801 cgcgacgggt tcctcaagac catggagcat ttcgatcaga cggtgggcat ggcgtatctg
     4861 cggggcctgc acctgaacga ttccaaggtc gcgctcggtt cgcgtgtgga ccggcatgcg
     4921 tcgctcggcg cggggctgct cggtctcgat gtcttctcct gcatcttgac ggatgcgcgc
     4981 tttgagaaca tgcccctcgt gctggagacg ccggacgaga cgatctggga acaggagatc
     5041 cggcagttga tggatatggc gggctgacgc atccggaaag gcgaaaggcc gcataaacag
     5101 gtggcgagac cccgcgccta ttcgaacgcc accaaatttc tttcgcgcag gctgacgtat
     5161 ccgggcgaga cggcggaggg tttgcccaca atcaggtgat cgaccacccg gatgcctaaa
     5221 atgcgcgccg cttcaaccag ccgccgcgtg acttcaatgt cctctttgga ggggatcgga
     5281 tcgcccgacg gatggttgtg ggccagaatc atggaggccg cgctgtagcg tacggctttg
     5341 ttgaagacct cacgcgggtg gaccggactg gagtccaaca ggccgcggga cgtttcgatc
     5401 ggctggccga tcaaccgact cttggtgtcc aataacagag tccaaaagat ctcctgctgc
     5461 agggtgcgcg tgagcgggag cagtacccgg taggccgcct ccgggtcacg gatcacgggc
     5521 gtgtcatggc gcggaccctg ttgggcggca cggcgtccga gttcgagcgc ggccgagagt
     5581 tcaagagcct tgaccttccc gaggcccttg atccggttgt gcaacagctc ttcgaaggtg
     5641 gttttcgaaa gcgcggcgag gccaccgtac cggagcagaa tctcgcgggc gagttcggtc
     5701 acgttcttgc cgggaagacc ggagcgtaac agaatcgcca aaagcagttc gtccggcagg
     5761 ttttccgcgc cacgccgctg gagttcctcg cgtgggcgca tctctaccgg caggtcgcgc
     5821 atcggcggcg ccgcatcggg atagacggta cgttggtcag aagggcaggg gggttgctcc
     5881 atgttgcctc catgctttga gaatccgttc cgcgatccgc tcgggcgggt cgcgctcgtc
     5941 ggtatcgatc caaaccgctt gtgtctggtg gcggaaccag gtttcctgtc gtttagcgag
     6001 ctgccgcgta cggatcacga tccgttcaaa ggcctcggcc tgtgagagcg tgccgtccag
     6061 aagggcgcaa ctttcggcgt atccgatcgc ctgcgccgcg ctggcggacc agactgggta
     6121 atggcggcgg agcgagcgga cttcctccac gagaccctgc gcgaacatgg cctcaacccg
     6181 cgttcggata cgccggtgga gctgctcgcg cggcatgcgc agaaccgtca acggcgcgtc
     6241 gggtgcgggc tgtttccagt tttgcggcag gctgcggagg ctctcgagtt gttcgatcgc
     6301 gcggatcagg cggcgcggat tggaggggtc ggagagggag gccagcgcct gggggtccag
     6361 agcggcgaga gcctcgcgca gggctccgac tccttccgtt tccaagcgcg cctgccaatg
     6421 agcgcggcgc gcggggtcgg ccatcgcgct gtccaatccg cgggtgaggg ctttgaagta
     6481 gaggcctgtg cctcccacca ccagcaggga ggggacagcc ttcccgtcgg agtcagcggc
     6541 attcgtatgc gcgacggcgc gggccgcctc tcgcaaccag gcgccggtac tgaagggttg
     6601 atccggcgtc acaaggtcca cgccgaaata ggtcacccgc gcacgttctg ccgcggaggg
     6661 tttggcggtg ccgatatcca tgccgcgata aaccagcatg gcgtcggcgg agaggatcgc
     6721 gatttccaat cgttccgcca agagttgtgc ggcggcgctc ttgcctgtgg ccgtcgcgcc
     6781 ggccagtgtc caagcgaggg tcatcgggtt gtcagtcccc tgtttccggt tccgtcatct
     6841 ggtcccgcgc catatgtcgg tcatggcgcc actgcatgat gaggtagagg aagacgccgc
     6901 agcagattgc cgaatccgcc acattgaagg cggggaaatg cgcgtcgccc caatggaaat
     6961 cgagaaaatc gatgacatgc cccagacgca accggtcaat gaggttaccg accacaccgc
     7021 cgaagagcag ggacatgatc accgtccggc cgcgtaatgc gccgaagagc gcgcggcgac
     7081 gccagatcat gaagatcagg gtggcgatcg aaaaaccgat caggaaggct tggcgtccgg
     7141 cgagcattcc ccacgccgcg ccctgattct ccacgtagct gaagctgaac aggccgggga
     7201 tcaccgacac cgatctcacc gcctgaagcc ggacgaccgc ccaatatttt gaaatctggt
     7261 cggccgccac cagcagggcc gcgaggagga gaacgcgcat ctcaaaacct tatctgcgtc
     7321 cgcggaaacg gaactgtttt tccatttcag attggcagcg gatgcagttc ttggcgaacg
     7381 gcaacacggc cagacggagt ttactgatca actcgccgca ggtatcgcac acgccgtaat
     7441 tccccttctc gatcgaccgc aacgcctcat cgatattcgt gatggtttgc agttggctgc
     7501 tgacctgttc cagggtttgc aggcgcatga aggcgttggt cccgtcctct tccgggttgg
     7561 tctcgtcggt gcgctgtagc gcggcgtggc gcatggcacc cgactgtccg gtgatgcgtt
     7621 cgcgcatgga gagcaggtcg cgtttgaatg cgttcaggtc gcgtttgctg aagtgggggc
     7681 taccgcgcac gggcttcttg gctacgagct tataagacgg cggtcctttt ttcacgggcg
     7741 cttcggttga ggtgccccgt tttacagcgg acttgcggac aacggttcgg gattgggtta
     7801 tggggggggt cttcgcggtc cgcgcaggtt tcgcctgcgc agccggtttc cgtgccgcgg
     7861 gctttttcgc tatggccgca gtggcgcgtt tggccggggc ggcgcgtttg gccggggcgg
     7921 cgcgtttggc cgcagtggcg cgtttggccg gggcggcgcg tttggccggg gcggcacgtt
     7981 tggccgtggc tttctgcacg ggcttggggg ggctcttctt ggtggagccg gtcttggttt
     8041 tacgggtagt tgccatcgga cacctcgctc tctcgataat ccgttttgca gtgaagaatg
     8101 gcgcattata cagggcaaga cgcgggatca caagccccga atccgaccgg gcggattctc
     8161 ttgatcccaa aggctccccg ggtcgaatcg cgtttcaaga catcgcgggg ggaggtgtgc
     8221 ggatccattc gcggatgggt tcgtccaagg gcagaaccgc ctcgatccgg gcagtttcct
     8281 cgccggacgg tccgggtata cgcaccgagt cgcctgcggt tcgccccgtg aggcagaggg
     8341 cgagacgcga tcggctggaa atgatgttca atgcctcatc acggtcccac tctccgagga
     8401 tcgtgtacac gatatgcgta ttgtcggcca gacatagcgc cacgcgtgtt ccgggacctg
     8461 ccgtttcgtg cggcgcgtcg gcgaagtcgg tccctttgac gagttggagt tcctgctgta
     8521 tttcagcctg acgctgtaac agttgccgct gaaagtcctt ggcggcctga tactcgaaat
     8581 tctcacgcaa gtcgccgtaa ctccgggcga tggcgatgtc ccggctgttc ttcaggaggt
     8641 cgacctcgat cagttttttg tactggaact gccgttcagc cagagagcgc caggaggtcc
     8701 agcgcacact ctcccgcgtt ggggccgggg cggctttttt gcgttcggtc agggacggat
     8761 caagttgcag catgcgtccc aacagggaac ggtgtgttgc gggatcccag gcgggcgagg
     8821 cctgaatgcg ctcaaagatc cgttggcgtt ggggcatgtc cagttccgcg aacaccgatt
     8881 ccagccatga ggtttggccg aaaaggcgtt tgagttggtt ctgcatgcgc agtgcctcgc
     8941 cggagaggcg gctttcgaca atctggatcg catgatccag cagttcgtgc agcggcggca
     9001 gccgccagcc gggcgtgtcg cggttgcgga aggcccaata aacgagcgtg ggcggcgcct
     9061 tgggctgagt cagcagtctg cggcacgcct cctccgcctc ggccgaagtg ttcagccggt
     9121 tcaacacctc gttgagcagg gagaacggca tctgcggcag ggcgtccagc aatcgttgcg
     9181 cggcggcgga gccttccgcg agcaggaagg tcgcgaggtc tgcggtgtct ttgacggtca
     9241 gggcttcgga tgcgcggagg aaacggtgct cttcccagag gtgcgcgcgc aactgatcgg
     9301 tcggcggggt cagaaatccg aagcgcgaga gcatgacggc caggcgggcg tacagtgccg
     9361 catcggactg atgcgcggcg ccgagggcga aatggagccg ctcttccaga atggcgcgct
     9421 gggccgcatc tagattttcg atccggccgg cagtctcaag ctcgatcgcc tgcgtaagga
     9481 ggcgctccgg gtttttggtc gcgctgaagg tggcgaacca ggcatcgccg tacgactcgg
     9541 cttcggtcag cagacggagc ggttcgcttt ttttagcggg gaagacgacc agcggatcct
     9601 gtttcagcgc cttgcgcgcc gcgtcccaaa accctttcca ctcgtccggg gcgagaatct
     9661 tttcctggac gagcaggtct tcgatcctga ccgcggtcag gtcgccgtaa ctccgcaagg
     9721 tcagccggag tagttcgccg ggctgctctc ttacgatctg cgcgaaacct ccgggatcgt
     9781 tatggcgcac ggtgagcaga tgatcttgcg gggcgggcac gaggctatcg caggctgtgg
     9841 caaaggggat ttcatggcgc ggtttccggg taaagtccac gatgaaacgt ttatagaagc
     9901 tgtccgcctc ctgaatgacc ccgaaccccc acgctggatc gatgacctgt gtgcccggtt
     9961 ccaacgcgag cagggtgccg aggcgtttca acgcttcgta aagcggcttc tctccgaact
    10021 gggctacatc cagcatcaca tcggtgaggc ggtccgggca ggtctgcttg aacaggcgca
    10081 ggatcgcatc gggtgcgagg gcgcaggaaa gcggctccac ctgatccatg acagtcagga
    10141 agagaccgcg gaaatccgtt gcgtcgacca gttcctcgat gagcagcatg gtccattctt
    10201 cagctttacc cgcgtctgtg gtggtcgtga tcgcctgaag caggggcaac agatccgtaa
    10261 ccgggagagg gcggatttca agcagggaaa agaatcgtgt ctcgagttct tcgaaagtca
    10321 ttgccgcatc catcataacg gaaccttctc ttccgtgcgt ttacagtttg gtctccggaa
    10381 tcttcggctt ggatgtttcc gcccctttcg cgccgctcgc cgcgagaaac gcgacacccg
    10441 cgtccatgaa aacccctcgt gaaaaattca tctcattctc ctctctttat acaccagagt
    10501 gataacaaat tcggaacggg agaggcaagc gtcggcttgt cagatgcggc caaagcccgc
    10561 agtagaagac aggagaggag aggtggggga ttggaacggg aaggacgcgc aattctcagt
    10621 tcgtaccctg aaacacatgt gaaagcaaaa agggaggggt ggaaagtcgg gagcaaggaa
    10681 aaactgcagc ggcaggggct tggtgcttga cttcgtggac tgcgtcaggc atcataaaat
    10741 tagaaacgtg gcacatggcg tttgcagagg ttctgcaagc acctttataa ctcgaaagcc
    10801 gttccaatgg aatggcatgg atggaagtcg ccatctgcaa agggaaaggc ggggcgtatg
    10861 aggatcatcg ctggcgcagt cgggttgatg gtggccacgg gtcatctgtg tgcgcgggtc
    10921 gagtcgcccg tcgtgtctct ggacaaaccc ctgccaagcg aggcgctggc gctgacgtgt
    10981 cccgcttcag ggacaagtga accggaaacg tccgcccccg gcgcgttccg gaccgcgaac
    11041 ctatcggcgg cggacagcca gtccctctgg gccgcgctct ccgaagcccg ccgcgaaatc
    11101 ggcgccgtcg gcgacgctgc ggacggcgcg ctgctgcgcg cgcacaaccc ggggcagagg
    11161 ctcgcggtcc gcttcatgcc aaacgctgtg agcgtcgccc cgtccggcac gaacgcggcg
    11221 tggcaggcct ttttcgcgct ggacggcacc gcagggcagc ccgccgctgt cgtctgccgc
    11281 ggcacgcgca tggaatacga ccgcggcagc ctcatcgaat ggtacgacaa tcaggccgga
    11341 ggcgtcgagc acggtttcat cctgaagacg cgccccgcag gggcgggccc cgacgtgcgg
    11401 ctgcgggtgg cggtcggcgg ccttcagccc aggctctcga gcgatggcga cgcggccgaa
    11461 ctggctgacg ggcaggggaa cgttctcctg cgatatgcca agctcaaggc gtgggacgcc
    11521 gacggccggg tactcgcgtc gcgcatggag gtcggggacg gcgccgtcgt cctcgtcgtg
    11581 gccgacgccg gggcgcgcta tcccgtcacg atcgatccgt ttatcaccgc gcagcaggcc
    11641 aagctggggc cggaggtgac cggcgacggc gcggctgaag attgttttgg ctactcggtc
    11701 gcgatctccg gcgacaccgc cctcgtcggc gcgtatcagg acgacgacaa gggcgccaat
    11761 tccggcagcg cctacgtctt cacccgcgac ggcaccacct ggtcccggca ggccaagctc
    11821 acagccgacg acggcacggc tttggactat ttcggctatt cggtggcact cgcgggcgac
    11881 accgccctcg tcggcgcgta ccaggacgac gacaagggcg acaattccgg cagcgcctac
    11941 gtcttcaccc gctccggcac aacgtggacc cggcaggcca agctcacagc caacgacggc
    12001 tcggacagcg actggttcgg ttattcggtc gcaatctccg gcgacaccgc cctcgtcggc
    12061 gcgtacgggg acgacgacaa ggtctccaat tccggcagcg cctacgtctt cacccgctcc
    12121 ggcagcgtct ggacgcagca ggccaagctc accgccgccg acggtgcagc cggcgactgt
    12181 ttcggctact cggtcgcgct ctccggcgac accgccctcg tcggcgcgta tcaggacgac
    12241 gacaagggct ccaattccgg cagcgcctac gtcttcaccc gcgacggcac cacctggtcc
    12301 cagcaggcca aactcattgc agacgacggt gaggtcggcg actggttcgg cacttcggtc
    12361 gcgctccacg gcgacaccgc cctcgtcggc gcgtaccgtg atgacgacga cggcaacgat
    12421 tccggcagtg cctacgtctt cacccgttca gacacgaact ggatccagca ggccaagctc
    12481 acagccaacg acggcgcgga aatggactat ttcggctatt cggtggcact cgcgggcgac
    12541 accgccctcg tcggcgcgta tgcggacgac gacaagggct ctatttccgg cagcgcctac
    12601 gtcttcaccc gctccggcag cacctggacg cagcaggcca agctcaccgc cgccgacggt
    12661 gcagccggcg actgtttcgg ctggtcggtc gcgctctccg gcgacaccgc cctcgtcggc
    12721 gcgtacgcgg acgacgacaa cggcgttaat tccggcagtg ccttcgtctt cacccgcgac
    12781 ggcacaacat ggaaccaaca ggccaagctc acagcgggtg aaagtgcggc cggcgacaat
    12841 ttcggcactt cggtcgcgct ctccggcgac accgtcctca tcggcgcgta caaggacgac
    12901 gacaagggca ccgattccgg cagcgcctac gtttttaccc gtgatggcac gaactggatc
    12961 cagcaggcca agctcaccgc agacgacggc gcaacctacg actatttcgg ctattcggtg
    13021 gcgctctccg gcgacaccgc cctcgtcggc gcgtacaagg acgacgacaa ggggtccgat
    13081 tccggcagcg cctacgtctt cgcccgcgat ggcacaacgt ggacgcaaca ggccaagctc
    13141 attgcaaacg acggcacggc cagcgactgg ttcggcactt cggtcgcgct ccacggcgac
    13201 accgccctcg tcggcgcgta cggggacgac gacaagggcg acaattccgg cagcgcctac
    13261 gtctttaccc gaaacggtac aacgtggatc cggcaggcca agctcaccgc cgccgacggt
    13321 gcagccggcg actgtttcgg ctgttcggtc gcgctctcgg gcgacaccgc cctcgtcggc
    13381 gcgtacgggg acgacgacaa gggcgacaat tccggcagcg cctacgtctt cacccgtgat
    13441 ggcacgaact ggatccagca ggccaagctt accgcctcgg atggcgcgaa ttacagctat
    13501 tttggtagcg cggtcgcgct ttcagatgat accgccctcg tcggcgcgta catggctgtc
    13561 aacgccgccg gcagtgccta cgttttcacc cgtgatggca cgaactggcc ccagcaggcc
    13621 aagctcaccg ccgccgacgg cgcggcctac gactatttcg gccgttcggt cgcgctctcc
    13681 ggcggcaccg ccctcgtcgg cgcggacggg gacgacgaca aggggtacga ttccggcagc
    13741 gcctacgtct tcacccgcga tggcacaacg tggacgcaac gggccaagct cattgcggac
    13801 gacggcgcga aaaacgactg tttcggctgg tcggttgcgc tctcggacga caccgccctc
    13861 gtcggcgcgt accaggacga cggtctggac gctttaggcg gcgcggctgc ggatcagggc
    13921 agcgcgtatg ttttcacgct ggtggatgac ataaccgtca cgctcgacta tcaatacggc
    13981 gggatcacga acaccctcat cttgcagacc agcggcacct atgcctcgct tccctcggcc
    14041 acgcgcccgc tgcatacttt tctcggctgg taccggacgc ccaccgacac actggaggac
    14101 atccagaacg gggtggacaa ccacatctat cccaccaaca cggtgaagcg tgccctcgcc
    14161 acgctctatg cccgctggag tccgttcacc gctacgcgca caacgcccat cagcatcaga
    14221 gtcaccgcgc tcgcgccctt ttaccccggc gacatcagtc aggcgttccc cgacgaggtc
    14281 gaggcctacg ccagcggcaa cgccgccgca gccgcctaca aggtctggga gctgatcctg
    14341 ctcggactca accccaccga tccggacgtc gtgcccacag ccatcctcgc ctacatcacc
    14401 ttcgacggga gcggaaacgc ggtcgtgcac aagatccccg cagtaggcat cccctccgcg
    14461 accatctaca agcaatgggg caagcagaat ctggccgacc ccgtctggac cgacctcggc
    14521 acccagggga cgccttgccc gccaagccaa ccctaccgct tcttcaagat caccggcgaa
    14581 tgagagagca ttgggagggg cagaaagtcg gaagcgcggg cgtccccgcc cgcaacagga
    14641 tgctcattag tgtatgacgg cttacacaaa cgcaataaaa acaagttgct tctcatctta
    14701 cctttcatga aatttccgtt ttatggtttc atccgaatcg ggatcgggat cgctatcggg
    14761 atcggctgtt tcaccacggg acacattctt gggtttcacg gtttctggtt tcagggtttc
    14821 aaggtttctg ggtttcaggt ttcaaggttt catccaaatc ggggtcggta tcgctatcgg
    14881 tatcgggatc gatcatttaa ccacgaacac acgggatggc actgctcggg ttttcatcca
    14941 cagatttaca cagattacgc agattttttg ggcatcgctc gggttttttg ggaacgccag
    15001 agggaattcc tgattttcat gaagtttcat gtgtttcagg gtttcatcca aatcggggtc
    15061 ggggtcggga tcgctatcgg gatcgattgt ttaaccagag gacacaagaa cacacggaaa
    15121 gtcatggctt tgtttcatac caagcgtgcg gcataaattg tggccggggc agtaaggggg
    15181 ccgggcgcgt ataggggttc gctgaaaaat atccattgcg caggaagcgg aaaagggtga
    15241 cactgaataa cagcgtttcg tccggtcttt cccgattggc cgggcgcggc aggattccgg
    15301 acagagaaag cgaggaggct tcatgaaaca tcatgtgatg gtggtgtgcg ggttggcggc
    15361 ggcggtgtgc ggtcaggcgc gggagatcgg atttatcgag acctttagcc ttgcggcgaa
    15421 ccgtgaacag gcgcttcagg aattggttcc gggtacggat gagtattatt actatcacgc
    15481 gctgcacgcg cagaataccg gtgcaaccga gcgctttcgg gaggtgatgg cccgctggca
    15541 gcgggagcgt gacggtgcgg tgaccgacag cggacgcgag ttgctgaacc gtcaggcgct
    15601 gcttgattac gacaaggaac cacagcggag cctcgactat ctgaagcgcg agctggatct
    15661 gcattttacg catgcccgga aaaccggcga gcgcaccagc ggtgcgccct cggttttcga
    15721 caacgcccga atcgcgcccg aggctttgct gaagcgtgcc ctagcagcga agccgcgtag
    15781 cctcgaccgg gtcgaggacg ccggattgcc gttcgctgct gctgccaagc tcaacgatga
    15841 acagcgacgc agtctgctgg cccgcctgcg ccgtccggat tatcccggtc tgacggcact
    15901 gatcgcggct gatctgaaag cccgcgacag ccgcggattc ggctctctcc cgattcatag
    15961 acttctgacg atcgagcaac tggatgagct ggtgaggaaa gagaacggtt tacgcaatgc
    16021 gaccgctttc gtgaacgcct acctcgctaa actcgcgcca gaggacgaag tggatctcga
    16081 caccgacacg gccgcgcgcg aagcctatta cgagcgggtt tggaatgttg tgaggcaact
    16141 cgaccccgtc cacaattcgc tcaaggcgaa tgtgctgtac aaccggttgc gccacgacca
    16201 gcgtaaaggt gtgtatgacc gcgaccgttt tctcgagtac gttaaattgc cgcgtgatgt
    16261 gcactacctg cgccccgaaa tccgccagca gttgccgcgc ggtgattatc tggcgcgtct
    16321 gggacaggac ttccgcctgg tctcgctgcc gccgatcgtc aacgaagaac cgctggtgcg
    16381 gaggtacctg ctgcgcttct tccgcgaaga cgctgatatt gaggcgtacc ggccctttct
    16441 ccgcgacgat tttcttaaac cgctgtttgc cgaggccaag atcgtccatg gcatcggcga
    16501 tccgcagcaa tgggtgaaac tgcttacacc ggacgcctac aaacggctca aggaacgggt
    16561 cgacatcgac tttgccgaag agaatcccaa cgaaatcggc gcggacgagg cggtgaagct
    16621 cacggctttt gtgaaaaacg tgccgtcgct gatcgtgaag gtctacgaga tcaacacctt
    16681 caactactac cgcgagacag gccggccttt ggatctggct ctcaatctgg atggcctcgt
    16741 cgcctccttc tcgcgacgtg tggaggttcc ggaacccgcc gagcgtcgtg tggcgcgcgt
    16801 gttcgacctg ccggaactca agggccgcgg cgcgtatgtg gtcgagttga tcggcaacgg
    16861 caagagtagc cgggccctgg tgcagaaggg acgcttgcgc gtgctgcaga ccgtgactgc
    16921 cgccggacat gcgtttacgg tgctggatga ggccggccgc cgcgtggcca atgcctgcgg
    16981 gtggctggga ggacgcgaat tcaaggccgg accggaggga cgcattctcg ttcccttctc
    17041 caccgcgccg aaaagcgaaa atcttgtgat ccgtcaaggc gacttcgcca ctctggtacg
    17101 cttcgaacac cttgcggaac aatatgacct cgccgccgga atttacgtgg accgcgaatc
    17161 attgctccgc cgcgagaagg cgcagatcgc catacgtccc gtgctgcgcg tgaacggcag
    17221 acccgcaagc ctcaaactgt tggaggcgcc cgtgctgaca gtccgctcaa ccgacttgca
    17281 ggggattgtg accgaaaagg agttccccga cgtgacgttg cgcgaagacg ccgagaccgt
    17341 gcaggaaatc ctggttccgg aaaacacggt cgaactccgt atgaccctga aggcgcagat
    17401 ccgcaacctc agcctcaacc aaaagcagac attgtcggat gacgcggtgt tcacgctgaa
    17461 cgggatcgag cgtcagcaga cggtacagga cctgcatgcg ggccgcacat cggccgggta
    17521 cttcgttgaa ctgcgcggta aaaacggcga gccgctggcg ggtgagccgc tcgcctgttt
    17581 tttcaagcac cgttggttcc gtgacgaact ccctgtcgaa cttcagaccg acgcgcaggg
    17641 gcgcgcgctt ttgggtgcgt tggacggcat cgcgtcgttc cgtattcaca ccgcttcgag
    17701 aacaaagcac acgtggaagc ccgagcgggg cgcgtgcgcc tatcccgagg cccttcacgg
    17761 caaggcaggc gagacgttgc gcgtaccgat cattcagggc gcggcggatg cggtcagcga
    17821 ggcctcgtta ctggagatgc gtaacgggca atttgtgaaa gattggcggg gcgcgctggc
    17881 ggcggcaaac ggttttctgg aattgcgcgg tctgccggca ggtgactatt cgctcatgct
    17941 cacggcgggg ggacgcgaaa tcgctgttcg cttgacgcaa ggtgacgagc gcgacgactt
    18001 cgtgatctca ccgcgccggg cgctggagcg tccgcgtctt cagccgctgc acgtgaccgg
    18061 ggtcgcgtct ggcgcggaga acgtggagat cacgctcgcg aacgtgacgc cgttcacgcg
    18121 catccatctc tttgcctcgc gttatctgcc ggcgtacgac gtttttgcgc ggctcggttt
    18181 tcccgggggg gcggggctgc tgcggcagtc atggcagtcg gtgcgcacct tctacgagtc
    18241 ggggcgcgac atcggcgacg agtaccggta tatcatcgag cgtcagcagg cggaaaagtt
    18301 tccgggcaac atgctggagc ggcccggatt gctgctcaac ccgtgggctg tacgcgagac
    18361 cgaggcgcag gctgaaaaac tggaggcagg ccgcgcgtac gcgggacaat cggccgccat
    18421 catgcaagct gacgggactg cgcggcactc tcaggcggcg gtcgtcgagc cgcccgaggg
    18481 atacgccgcg ctcgatttcc tgaagcagcc ggccgtgacg ctgttgaatc tggttccgga
    18541 ttcgacgggc agggtcacgg tcccgcgcgc cgcactcaag gacttgcctt tcctgcgcgt
    18601 catggcggtt gatccgctct ccacggtgtt ggtgtgtgcg gcgttggagg acacgccggt
    18661 cgccttacgc gagttgcgcc ttgttgcggg gctggatccc caaaaaagct atgccgaaca
    18721 aaagcgggtt acggcagtgg ctgcaaaaga gagcgtgacg atcgaggatg tgaccacggc
    18781 gcggttcgag cggtacgata cggtggcgaa ggtgtaccgg ctcttggcca cactcaatcc
    18841 ggacgcgacc ttcggcgagt tcgctttcgt ggccaactgg ccgaagttgg agtccggaga
    18901 acagcggcgg ctgtactcca agtatgcctg ccacgaactc aatttctttc tgtaccacaa
    18961 agatccgagg ttcttcgagg cggtcgtggc accgtatcta aagaacaaga aagacaagac
    19021 gtttttggac cgctggttgc tgggagacgc gctcgaagca tttttggagc cgtggcggtt
    19081 cgcacggttg aatgcggtcg aacggatttt gctggggcaa cgtttgcgag ggcagagggc
    19141 gtcggtggcg cgcgacacgc gcgaacgggc agcgctgctt acgccggaca tggaggcgtt
    19201 caaccgacgt ttcgatacgg ctgtgcagag cggggcgctg gaggcggaaa aagacggcgc
    19261 gtctggtacg gtggaggcga ttcgtaaaga acaggcggac aagcaagcgg tcggcttcgg
    19321 gaagcaggtc ggcgcacctg ccgctgccgc gctggtctcg tctccggtgc cctccgcgcc
    19381 ggaaccgatg ctggaagcgg ccgacgcgtt taggaaggct gagccggata cgcgccgggc
    19441 cgaggcgaag aaaaaggagg cttccggcat gccgcgtcag cgggcgttgc ctgaggcgtc
    19501 gccccaagga cgggctgtcg atgatccctt cgcaccggct gagaaggagg aggctgagtc
    19561 gttgttcttt gatgcgggtg agcaggaccg tgccgcttcg cgccgtttct tccgaaaact
    19621 cgacaagacg tgggagttgg ctgagaacaa ctactaccgc ctgccgatcg agcgtcaggt
    19681 cgccgacctt gtgaaggtca acgctttctg gaacgactat gcggcgcacg acggcagaac
    19741 accctttctt tcgggagcgc tgccagaggc cacctcgaat ttcaccgaga tgatgttggc
    19801 gctggccgtg atcaacgtgc cgtttcaggc agccgcgcat aacgaaaagg tggaggggct
    19861 ccagtatgtg ttgcaggccg ggtcgccgct gctgctgttc catcgcgaga tccgcgagac
    19921 gccccgtacc ggggcgccgg gcaacgtgct ggtggcccag cactttttcc gggcggatga
    19981 ccgcgggcgc ttcgagaaca acgatcggtt cgacaagtgg gttacggacg agttcctgcc
    20041 cggggtcatc tacggggcac aggtggtcat gaccaacccc accggtaacc gccagaagct
    20101 tcaggttctg ttgcagattc cggtgggtgc gcttccggtg gccaacggat tctacacacg
    20161 cggtgcgtat gtggtgctgg agccctacag tacgcagatg aaagagtatt ttttctcctt
    20221 cccgtccacg ggtacgtttc cgcattatcc ggtgacgttc gccagcaacg atcaggtggt
    20281 cggcggcacc gaacccttcc gcttcaacgt cgtgtcccgg ctcagcaagg ccgacacgac
    20341 gtcgtgggcg tggatttcgc agaacggcac cggggccgag gtgaccgcgt ttttgaatgc
    20401 ggccaatctg caccgtgttg agttggaaga gatcgcctgg cggatgaaag agcacgcctt
    20461 cttcaagtcg gtcaccgggc tgctgcgggc gcgccacatt taccacgaca cgctttggtc
    20521 ttatgggatc ctgcacaacg agccggaggc gatccgcgaa tatctgcggc atacgccttt
    20581 tgccaaccgg tgcggactct ggctggcttc accgctgttg acgctcgatc cggtcgagcg
    20641 cttcgactac cggcatctgg agtatgcgcc gctggtgaac ccgcgcgcgc accgggtcgg
    20701 tccgaaacgc accatccgga acgccgcctt ccgcgagcag tatcagcgcc tgctgaaggt
    20761 gttgagcttc aagccggagt tggacgcgtc cgacacgctg gccgtaaccg cgtatctggt
    20821 gttgcaggac cgcgtggccg aggcgctcga ctggttcggc cgggtggacc gcaaggctgt
    20881 gccggaacag cttcagtgcg actatctcga agcctacctc gccttctaca agggcgacat
    20941 cgagcttgcg cgcaacctcg ccacgaaaca tgcgggcgag ggggcggatc gttggcgcga
    21001 ccgtttcgca ctgatcctga aacagatcga tgagatcgcg ggcggcacgg ccgatatggc
    21061 ggacacggag agccgcgacc cggttcagga cgcgctggcg gcgaccgagc ccatgctgga
    21121 gctgcatgtc gaggcgggcc gcatccggct cgacacgcgc aaccttacgg cttgcacgct
    21181 gaacttctat cccatggaca tcgaattgct cttttcgcgt aacccgttcc ttcaggaggg
    21241 cacggcgcag ttctcccaca tccggccggt actcagccgc acggtcgcgt tgcctgccgg
    21301 acagaacggc ctgaccgtgg atttgccccc ggagttcgcg ggcaaaaatg tgctggtcga
    21361 ggcattggcc ggcggggcgc gcagaactca ggcctattac gccaacacgc tgaaggtgcg
    21421 gaagatcgag agctacgggc agctcgtggt gacgcagacc gcgaccggca agccggttcc
    21481 gggagcgtac gtgaaagtct acgcgcggca accgggcggc gaagtgaagt ttttcaagga
    21541 cgggtacacg gacctgcgcg gccgttttga ttacgtttcg ctgaacaccc gggaggcgga
    21601 gacggccgag cggttcgcgg tgctggtgct cagtgacgct ttcggcgcga tcgtgttcga
    21661 ggcgccgccg ccgaagcagt gaacgttgaa ggaaaaacat ctgttttgcg ttttctatgc
    21721 cttgacagaa ccggatcaag ttcttacgtt aagaggctct tgcaaaaccc tcccaaacgc
    21781 aggccgtcag gcttgcatac ggga
//
LOCUS       DAOSRB010000023        21615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2626_length_21615_cov_8.384694, whole genome shotgun sequence.
ACCESSION   DAOSRB010000023 DAOSRB010000000
VERSION     DAOSRB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21615
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2626_length_21615_cov_8.384694"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..351
                     /gene="secE"
                     /locus_tag="PLZ74_02425"
     CDS             148..351
                     /gene="secE"
                     /locus_tag="PLZ74_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOR97209.1"
                     /translation="MSKVKESIRSFVAYLGEVGVEFKKITWPDRQELVDSTVVVLVFI
                     VILAVVVLCCDKTILFFLQLIHA"
     gene            365..928
                     /gene="nusG"
                     /locus_tag="PLZ74_02430"
     CDS             365..928
                     /gene="nusG"
                     /locus_tag="PLZ74_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOR97210.1"
                     /translation="MTKQWFVLHTLTGQEQKVKRSIEARVRIEEMGEYIGEVKIPTEK
                     VSEVKNGVKKTVTRKFFPGYVLINLALYDEARNVIEPTWRFLKETGGVIGFIGGSRPL
                     PLSPDEVDSIVNQAAEKKEKVVPKVQFEPGEEVKITDGPFMSFSGTVDEVDPDRGKLK
                     VSVAIFGRTAPVELEYWQVERAVSDRA"
     gene            1040..1465
                     /gene="rplK"
                     /locus_tag="PLZ74_02435"
     CDS             1040..1465
                     /gene="rplK"
                     /locus_tag="PLZ74_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOR97211.1"
                     /translation="MAKKITGTVKLQIPAGAANPAPPVGPALGSQGINIMAFCKEFNA
                     KTQDKAGLIIPVVITVYADRSFSLVFKSPPASVLLKKEAGLAKGSGVPNRDKVGKVTR
                     EQVRKIAALKKADLNASSEEAAMRMIEGTARNMGIEVVD"
     gene            1484..2176
                     /gene="rplA"
                     /locus_tag="PLZ74_02440"
     CDS             1484..2176
                     /gene="rplA"
                     /locus_tag="PLZ74_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOR97212.1"
                     /translation="MAKHGKRYVDALKKAPQGPVSLEEAVAFVKANPGAKFDETVEVA
                     MRLGVDIRKSDQTVRGTVTLPHGSGKKVRVVVFAAGNHADEAKAAGADEVGYEDLIEK
                     VKNGWTDFDVAIATTDAMKEVRKIARVLGPRGLMPNPKTGTVTDDTAAAVKAAKGGKV
                     DFRMDRTGNVCVLCGKRSFDAEKLVENIKAVVDAVRAERPAGAKGTFIRSLTVSATMG
                     VGVRVLVKEAAE"
     gene            2189..2713
                     /gene="rplJ"
                     /locus_tag="PLZ74_02445"
     CDS             2189..2713
                     /gene="rplJ"
                     /locus_tag="PLZ74_02445"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOR97213.1"
                     /translation="MRTEKVSILNEVIERVKDSDFCFVLNYGGLSVAQLTALRKELAK
                     HDARAMVVKNTYLGRVAKEQGWADVSGMLTGPTAIITGRGDVAEVAKALVEFVKKNDK
                     AAIKGANLDAAMLSAADVEALSKLPSKDAMRGILLGTLQAPATSLVRVLNAPLLNVLY
                     ALKAYEEKKNGSAA"
     gene            2866..3234
                     /gene="rplL"
                     /locus_tag="PLZ74_02450"
     CDS             2866..3234
                     /gene="rplL"
                     /locus_tag="PLZ74_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOR97214.1"
                     /translation="MTNEEVIDFLSNLSVLEISKLVKALEEKWGVTAAAPVAVAAAPA
                     AAAAPVEEKTEFDVVLVEAGANKISVIKEVRAITGLGLKDAKDLVEAAPKPVKEGVAK
                     EEAAKLKEQLEKAGAKVEVK"
     gene            3577..7428
                     /gene="rpoB"
                     /locus_tag="PLZ74_02455"
     CDS             3577..7428
                     /gene="rpoB"
                     /locus_tag="PLZ74_02455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOR97215.1"
                     /translation="MVDRKVFGKLKPVMQPPDLIAIQTGPYQDFLQRDVAPLKRENKG
                     LQAIFHEVFPIDSFDGRYTLDFVKYDLGEPKMDALTALVEGNTYAAPLHATFRLKDGD
                     EIREEDVYMGEIPLMTRDGAFVINGAERVIVSQLHRSPGICSERTQHPNGQTLYSVRI
                     IPDRGSWIEIQFDSSDLMWVYMDRRRRRRKFFVTTFLRTLGYGTDEDILKLFYTFTSL
                     PVNKEFTDEELEVLVMKDDLIDVESQAVLARRYDPITPALLEQIGKAGFSKIEVVDVS
                     FDGGVLIKTLREDAKANIYSEEDALKDIYKRLRPGDPATASNAKALLKRLFFEPRRYD
                     LGKVGRHKINQRLGLSGKVPDTLRTLDESGVDVVEAVRLLLKIYTGEETVDDIDHLGS
                     RRVRTAGELLENQCRVGLARTERLIRERMTLFDPASGVLAPARLVNSKSLSAVVQDFF
                     GRSQLSQFMDQTNPLSGLAHKRRLSALGPGGLSRERAGFDVRDVHASHYGRICPIETP
                     EGPNIGLISSLCIYSKINEYGFIESPYRVVRNGKVTKEVQYLSADKEEEFVIAQANSE
                     LKSDGSFVSQRVTCRYKTEFVVVDRSSVQYMDVSPMQVISIAAGLIPFLEHDDANRAL
                     MGANMMRQAVPLLQSESPFVATGLEARAASDSCVTVLATEDGVVAYASATEIIVTPDG
                     KMPPKTKKGEAIKEAGRYSLQKFRRCNAGTCFNQKPVVEVGQKIRKGDVLADGPATYG
                     GELALGKNLLVAFMPWTGYNFEDAILVSERIVREDIFTSIHIQEFEVGARDTKLGPEE
                     ITRDIPNVGEEALKNLGPDGVVRIGAEVRPGDILVGKITPKSETELAPEERLLRAIFG
                     EKAADVRDTSLTVPSGVYGIVMDVKVTSQQDAHRPFLSLTASSEEKREVRDIEEERKK
                     KQAALEEELTTALSNILLGEKIPLDVLDVENGEIIIPANRKITKTLLARLAKAHDHIQ
                     IENSPVQQKIEEIINGFRPKFADLAEQSSNDFGAELDGIIDEGGVIKQVRVYIAEKKN
                     LSVGDKLAGRHGNKGVVAKILPDCDMPFLPDGTPVDIVLNPLGVPSRMNLGQLFETYL
                     GVACKVLGFHASSPVFDGVSEEEIDALLRQARKVQESEIGGSAWICEDGKTVLYDGRT
                     GEPFDQRVVVGHIYMLKLHHLVTDKIHARATGPYSLVTQQPLGGKAQYGGQRMGEMEV
                     WALEAYGVAYALQELLTVKSDDVSGRTRIYEAIVKGQNTLDAGMPESFSVLMHELRGL
                     CLDMKLLGSDTAGPTPELSRI"
     gene            7450..11640
                     /gene="rpoC"
                     /locus_tag="PLZ74_02460"
     CDS             7450..11640
                     /gene="rpoC"
                     /locus_tag="PLZ74_02460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOR97216.1"
                     /translation="MNPYASREMIGGTYDANAHYDAVSITLASPETIRSWSHGEVRNP
                     ETINYRTYRPERDGLFCERIFGPTRDWECNCGKYKRIKHKGVVCDRCGVEVTVSRVRR
                     QRMGHIELAVPVSHIWFFKCSPSRIGLILDKTANELERVLYYEDYMVIDPGEKSGLEK
                     AQLITESEYHDYVEKYGESFTAMMGAEAVRRVLNDVDLDLLMQTLEEQIENTRSKQTR
                     KKITKRMKIVEGFRTSGARPEWMILDCLPVIPPELRPLVPLEGGRFATSDLNDLYRRV
                     INRNNRLKNLLSLKTPDVIIRNEKRMLQEAVDAVFDNGRHGRAVAGAGNRPLKSLSDM
                     LKGKTGRFRQNLLGKRVDYSGRSVIVVGPELKITQCGLPKKMALTLFEPFIIRRLKEK
                     GICHTVRTARKMIERHDEQVWDILEEVTKGKTVMLNRAPTLHRLSIQSFEPVLIEGEA
                     IRIHPMVCTAFNADFDGDQMAVHVPLSVEAQLESRLLMLTSNSIFSPASGKSIMTPTQ
                     DIALGIYYLTIPSQSDKLANKIAGKVPAGAERLPLFNTTAEVDSALAEGAITYHSRIR
                     LRNPDFGTQDRRFGNPEPSVIETTAGRVIFNEVWPDELGFVNDKVDKNKLGDLILHCH
                     QVAGHARTVKALDELKTLGFKHACLAGVSMGLKDMIRPKNKDEIIAQAHSEVEKVEAQ
                     FQTGVITDGERHNKIIDIWTAATEKISDELYKAIDRNVTESVSEPNETNPVFMMVDSK
                     ARGSKLQIRQLAGMRGLMANPSGEIIERPITASFREGLSVLEYFISTHGARKGLADTA
                     LKTADAGYLTRKLVDVSQDVIITIQDCNTVNGIEVEAVEEGDEMLIPLRTRVLGRTAL
                     YDIRHPATMELLVAANAEIDEAACKALEDAGIERVWIRSGLTCDAPHGMCAKCYGRDL
                     SSGKLVEIGTAVGIIAAQSIGEPGTQLTMRTFHIGGTASTVSKIPEIVLKNAGFIKYV
                     DLRIVQNDEGNTVVLNKNGTIEIVDANGNEIDTYNLQMGTVLLAADGAAVKAGQRVAV
                     WDPHSVPILADKHGVVSFQDFVDDVSVRSDQDAETGATTLLVLDTKEDLHPQIIITDA
                     KTGEILGFYHIPVGAVVMVKEGTKVSPGMLLAKTPRKEAKTRDITGGLPRVAELFEAR
                     QPKDAAEISRIEGVVDFGEHQRGKRCLIVRDEGGHDQEHLIPMGKQVVVFKGDRVKKG
                     QQLTEGPVVPQEILDVCGPQELQRYLVNEVQQVYRLQGVEINDKHIEIIVRQMLRKVR
                     ITNPGDTDFLWGEQVSRQKFLEVNEQVLASGKRAAEAQPALLGITKASLETDSFISAA
                     SFQDTTRVLTDAATMGRVDELRGFKENVILGHLIPGGTGFPLHRHIKLVPLADPISEE
                     EMAALQADAQAQFDEVFN"
     gene            complement(11707..14655)
                     /locus_tag="PLZ74_02465"
     CDS             complement(11707..14655)
                     /locus_tag="PLZ74_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOR97217.1"
                     /translation="MIRYLRAVIALSAVVFTAALVRAEGLALESPGQKLQAVFALSPE
                     GTLTYRVTFGGVPVILPSKLGFTTVDRTDLTLGYALVSAKRATIHQTWKNPFGERAQV
                     TDAYNALTLILRNRAANDLTLHLECRAYDAGLAFRYVFPQQSLTQLLFAKENTEFIFT
                     GDHRAWPVYSAQAAYKPARLSEIRNGCERPLTVETAEGPVAAIGEAGLLTFARMKFAP
                     LAGTPHALTARLDGQASLPLPGATPWRFIMVAENAARLLEQNDLLLNLNEPSRIADLS
                     WIRPGKVIRETSLTTAGGKACVDFCKTMNLQFVEFDAGWYGHEYDDAADARGVNLDPK
                     RSKGPLDLPEVIRYAKARDVGVILYVNRRELERRLDELLPLYASWGVVGMKYGFVQVG
                     SQTWTTWLHEAIRKAGEARIMIDIHDEYRLTGNQRTWPNVMTVEGIRGNEEMPDAAHN
                     CALPFTRYLCGPGDYTPCWYNNRVKNTRAHQLALAAVTFSPWQFLFWYDRPSMYQAEP
                     ELDFWRQIPTVWDDTRVLHARIGAYASIARRSGETWFIGSINALERRSLDLPLSFLSP
                     NVRYTAHVYSDAAPDGSDRTRVACATRIVTSADTLTADMAENGGHAVRLTPVAEQPVC
                     PAIREALRGSLDSHEVAGAVSLVLTADRTLHFDACGHADLAAKKPMTPDAIFWIASMT
                     KPVIASAILMLQDEGKLSIDAPAATHLPELANLKTADGRGFSPTVKHLLTHTSGLSEP
                     TQAEAFAARTLAELMPHIVSKPLRFEPGTKWQYSQSGINALGRIVEVVSQQSLPAFLK
                     ARLFDPLGMRDTTFYPDAAQCARIAKAYRQVDGRLEEADMRALYDFRRGPDRYPAANG
                     GLYSTAADYGRFCRMLLKRGTAGDRRILSTEAVAAFSSLQSGELKTGFTDGMGWSLGC
                     GLVRQPQGVTAKLSPGTFGHGGAYGTQAWIDPVRGAAYILMVQRADFPNADASEIRRA
                     FQDAASAIVP"
     gene            14985..15650
                     /locus_tag="PLZ74_02470"
     CDS             14985..15650
                     /locus_tag="PLZ74_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97218.1"
                     /translation="MARNGVRAFSLPGNRGRKRKMKRKRAAYAYATIASAAALAASAA
                     TLDVPRLPEPAFADREASAAHALPAGRKDGLRTFRLELSFGATPSNNVQAAFGRDGVP
                     ADGALDAGETDLIIGWDRGEWFIAPRGLRGRHAAPAATSNGLQRLTALIRVTGAGVCL
                     PPSFADRDGAVVFAGLPADPFPEWLRPDAWDTLRVTARGADAPLESVRAVFAADGALL
                     KLR"
     gene            complement(15643..16053)
                     /locus_tag="PLZ74_02475"
     CDS             complement(15643..16053)
                     /locus_tag="PLZ74_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97219.1"
                     /translation="MDDRVGGGRQPRGGVGGHARQREARGVLGGGEGAGGGAAGGGRR
                     RGRRAPRRGPPALGGGRGRAGGRDGFRDGAEAEGRGENQENRPVDAGQGAEETAAAVA
                     LAGLVRSGREPERGSGAEENEKCFGCHCRASLFI"
     gene            complement(16404..16610)
                     /locus_tag="PLZ74_02480"
     CDS             complement(16404..16610)
                     /locus_tag="PLZ74_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97220.1"
                     /translation="MKSSSYVGMPSPSWSLTDGSSMPKARQRSSRPPAGAEGSPGAKV
                     SPPWLCRAPMKKLVSAGASVKAYR"
     gene            complement(16983..17219)
                     /locus_tag="PLZ74_02485"
     CDS             complement(16983..17219)
                     /locus_tag="PLZ74_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97221.1"
                     /translation="MRPGGASSAKGSAIFQTVRFGREGCRKETDAARGPAPSRCAVTV
                     SVWTAEEKSPEAERALALVAHDQTAAPPRETQTA"
     gene            18001..>21615
                     /locus_tag="PLZ74_02490"
     CDS             18001..>21615
                     /locus_tag="PLZ74_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97222.1"
                     /translation="MWRSGGGCGEYLSETGGFTVTAGSDEVDALVTFLVNGEECPSRS
                     RAWQGGETSAEVSVTYGGATLWSASAAREPASFDGCGEGTGWEGEDAGGGECGCGCAD
                     GGCDSLEGDEIGSVRFRVPLGETGRGRLAGFVWFEITEPRAVTPALFEVAGGASVLCA
                     SNNPGGALSDVSCPEPGGRRVAVAPAPGGAALAVYAGHGDADPSHSWEITNPSGAGTV
                     RVVRRDGAGNITRDALYTHADHGWGASWTREDLLTGLTESVLYHDPPAAGGHVCEMRT
                     ATDSGGARVLSATEVYRAEVGSGPAAVTRETRRWEWDAALGSGIEVSMTWWEDSLNPL
                     RNGKPKFRQGGGGEWEYRAFDARGREVLRAGPLDGSGPPPFASDPAPFALSGVPARPP
                     FDALVTETSYEPLPGDSGAPGDSREPRRISAYAVRGGVPAPVSREWRVFTRGASGGLP
                     AVTRRVIRAASPSSPPEDPANAVSVTVSAAGDDPSVPEPLRGRPLLEVPEDGATNSTS
                     YAFGGYDPGSRTFTEHPDGAFLRAVSSAGSRPLASAEVSDAAFGRVLLRETRLAAGGA
                     LLSWEARTHDALGRHLMSAFSDGTAETSLWGCCGLDGETGRDGARRDFLAVPGLPLWS
                     AEAGASAGALPGADGRHPVTETFRDALGRVTNTVRRVWLNGMRDPASAPLETRVEYPH
                     GSGRLRVTTGPSGVRTVTRRYFGGSGETEETVSPGVTSRVTRIRGGATVAETFRDGKR
                     TRETRSASHGADGLRVETVVTESSDGPAVTNSVTAYDFLGRVVSVATPSGVASNFYDG
                     PSGRVSRRTETGKPDTLYEYDAGGAGVPARTALDVDGDGRISYAGPDRITATHETYEQ
                     DASNVWWRVTSQAVWAETNSAACVTSSVIRVRLTGLGTPAPDALELSGGAILTAQTET
                     LDRRGNVTRVSTYTDAGAAAVWTVTETPGSVQPAIQKTVAGRTVSMVSSTAVTNAYTY
                     DGFGRQVSATDGRGNTTVTAYNELGQVAYTVDAAGNRTSYAYDGLGRKIAVTDPLGNT
                     THTAYDSAGNVTAQWGAAYPVAYEYDTAGRRIAMATTRDDTFDFSSVTNSSILNPNSS
                     LDVTRWLYDNATGLLTNKVYADGSRVRYTYTEGGLLATRVWSRGVTTAYSYDALGQLI
                     NINYSDATPDVAFAYDRMGNMVSVTDASGIRVFTHDPDGKMLSDTLSASGQEFALLES
                     H"
BASE COUNT         3889 a         6953 c         7253 g         3520 t
ORIGIN      
        1 gagtaccggt ctccaaaacc gggtgttggg ggttcgagtc cctcctggcc tgccatgtac
       61 cgctgcggca gaggacgcgg tccagctgaa aaaagctgcg gggttttgtg tcctgaagga
      121 aggcggattg aacgaggttc ctgaaacatg agtaaggtga aagagagcat ccggagtttt
      181 gtcgcgtatc tcggcgaagt cggggtcgag ttcaaaaaga tcacgtggcc tgatcgtcag
      241 gagctggtgg actcaaccgt cgtggtgctc gtgttcatcg tgattctggc cgtggtcgtg
      301 ctgtgctgtg acaagacgat cctgtttttc ctgcagctca tccacgcata atctggaggg
      361 ggccatgacg aagcaatggt ttgtgctgca cacgctgacc ggccaggagc agaaggtcaa
      421 acggtcgatc gaggcacgtg tgcggatcga agagatgggg gagtatatcg gcgaggtgaa
      481 gatccccacc gagaaagttt ccgaggtgaa gaacggggtg aagaagaccg tgacgcgcaa
      541 gttcttcccc ggatatgtgc tgattaatct ggcgctgtat gatgaggcgc gcaatgtgat
      601 cgagcccacc tggcggtttc ttaaggagac ggggggggtg atcgggttca tcggcggttc
      661 gcgtccgttg ccgctctccc cggatgaggt ggactccatt gtcaatcagg cggcggagaa
      721 gaaggaaaag gttgtgccca aggttcagtt cgagccgggc gaggaggtta agatcaccga
      781 cgggccgttc atgagtttta gcggcacggt cgatgaggtg gatcctgacc ggggcaagct
      841 gaaggtctct gtggcgatct tcgggcgtac ggctccggtc gagctggaat actggcaggt
      901 cgagcgcgcg gtcagcgacc gcgcgtaagg cggcttgcgg gaaccggcgc gcgcggcgcg
      961 ccggcggctc gtcaactgcg gcccgttcgc accggtgggg atgccgggag ggccgcgaca
     1021 gacacgaaac agggtaagta tggcaaagaa aatcacgggt accgtgaaac tgcagattcc
     1081 cgcaggcgcg gcgaatcccg caccgccggt gggccccgcg ttgggttccc aaggcatcaa
     1141 catcatggcg ttctgcaaag agttcaacgc caaaacccag gacaaggccg gcctgatcat
     1201 cccggtggtg atcacggtgt atgccgaccg cagtttttct ctggtgttca agagtccccc
     1261 ggcatccgtg ctgctcaaga aggaggcggg tctggccaaa ggttcgggcg tgcccaaccg
     1321 cgacaaggtc gggaaggtga cgcgcgagca ggtccgcaag atcgcggcct tgaaaaaggc
     1381 ggatctgaac gcctccagcg aggaggcggc gatgcggatg atcgaaggca ccgcgcggaa
     1441 catgggcatt gaggtagtgg actgagaagg ggggacgcga aacatggcca aacatggtaa
     1501 acggtatgtc gatgccctga aaaaagcccc ccaggggccg gtgagtctgg aggaggcggt
     1561 ggccttcgtc aaggcgaacc cgggtgcgaa atttgatgag acggtggaag tcgcgatgcg
     1621 cttgggcgtg gatatccgga agtcggatca gacggtccgc ggcacggtga cgctgccgca
     1681 cggttcaggc aaaaaggtcc gcgtggtcgt tttcgccgcg ggcaatcacg ctgatgaggc
     1741 gaaggccgca ggtgcggacg aggtcggcta cgaggatctg atcgagaagg tcaagaacgg
     1801 ctggacggac ttcgatgtgg cgatcgccac gacggacgcc atgaaagagg tgcgtaagat
     1861 cgcccgcgtg ctgggcccgc gcggcctgat gcccaacccg aagaccggca cggtgacgga
     1921 cgataccgcg gcggccgtga aggcggccaa aggcggcaag gtcgatttcc gcatggaccg
     1981 caccgggaac gtttgcgtgc tttgcggcaa gcgcagtttt gacgctgaga agctggttga
     2041 gaacatcaag gcggtggtgg atgcggtccg ggccgaacgt ccggcaggcg ccaaaggcac
     2101 cttcatccgg tcactgacgg taagcgccac catgggcgtg ggcgtgcgtg tgctggtcaa
     2161 ggaagccgcg gaataaggag gcggaagcat gagaaccgaa aaagtttcca ttctgaacga
     2221 ggtgatcgag cgtgtcaaag actcggattt ctgcttcgtt ctgaattatg gcgggctgag
     2281 cgtggcgcag ttgactgcgt tgcgcaagga gctggcgaaa cacgacgcgc gcgcgatggt
     2341 ggtgaaaaac acctatctgg gccgcgtcgc gaaagagcag gggtgggcgg atgtttccgg
     2401 catgttgacc ggcccgaccg cgatcatcac gggacggggc gatgtggccg aggtggccaa
     2461 ggcgctcgtc gagttcgtga agaaaaacga caaggcggcg atcaaaggtg cgaacctcga
     2521 tgccgccatg ctgtcggccg cggatgtgga ggcgctttcc aaactgccgt cgaaggatgc
     2581 gatgcgcggc atattgctgg gcaccctcca ggctccggcc accagccttg tgcgcgtgct
     2641 caacgcgccg ctgcttaacg tcctgtatgc cctcaaggca tatgaggaga agaagaacgg
     2701 atctgcggca taaaccgtgt cgtaaaaacc gcgggatggg agggcggcgg gtgccggctc
     2761 tcccgggtgc ggatacgggc cctgccgctt gtgaagcggt gatgcccgcg ggaaccccgc
     2821 acgcggcaga acgcgtgcgg ttgcacaaaa aaaaggaata caaccatgac gaacgaagaa
     2881 gtgattgatt ttctgagtaa cctctcggtg ctcgaaatct cgaaactggt caaggcgctg
     2941 gaagagaagt ggggcgtgac cgctgccgcg ccggtggccg ttgctgcggc acctgccgcg
     3001 gccgccgctc cggtggaaga gaagaccgag tttgatgtgg tgctggttga ggccggcgcg
     3061 aacaagatca gcgtgatcaa agaggtgcgc gccatcacgg gccttgggtt gaaggacgcc
     3121 aaggacttgg tcgaggccgc cccgaaaccg gtcaaggagg gtgtggccaa ggaagaggcc
     3181 gccaagctga aggaacagct tgagaaggcc ggcgccaagg tcgaggtgaa gtaaccgctt
     3241 cgtcgccacc gcttaacgaa cggggccgtc cgcatgatga cccggacggt cccgtttccc
     3301 ggcgaccggc ctctgcgccg cgaaccgggg aatgcccgta ccgtcatcaa accgcaacgt
     3361 tgcttgaatt gagaagaaac gcacctgaat ctgcctgaat cgcgtgcagc gcaaccgaag
     3421 tgtgaatcag ttaatcaagt ggctgcttac cacacttcca ttcataaccg gttgccatcc
     3481 gtcaccggca ggcatctcag cgggcgggcg gattttccgc cgtgcggatc tcgatccggc
     3541 gacgcgctta ttcatatcca accttcgagg taggcaatgg ttgaccgtaa agtctttggc
     3601 aagctgaaac ccgtcatgca gccgccggat ctgatcgcaa ttcagaccgg gccgtatcag
     3661 gacttcctgc aacgggatgt cgcgcctctc aaacgcgaga acaaggggct tcaggccatt
     3721 ttccatgagg tgttcccgat cgacagtttc gacgggcggt acacgcttga tttcgtcaaa
     3781 tacgacttgg gtgagccgaa gatggatgcg ctcacggctt tggtcgaggg caacacctac
     3841 gccgcgccgc tgcacgcgac cttccgtctc aaggacggcg acgagattcg cgaagaggat
     3901 gtctacatgg gcgagatccc gctcatgacg cgcgatggcg cattcgtcat caatggtgcc
     3961 gagcgtgtga tcgtctcgca gttgcaccgt tctcccggga tctgctccga gcgtacgcag
     4021 catcccaatg ggcagaccct ctattccgtg cggatcatcc ccgaccgcgg ctcctggatc
     4081 gagatccagt tcgattcgag tgacctgatg tgggtctata tggaccgccg ccgccgccgc
     4141 cgcaagtttt tcgtgaccac cttcctccgt accctcggct acgggacgga cgaggatatc
     4201 ctgaaacttt tctacacctt tacgagcctg ccggtgaaca aggagttcac ggatgaggag
     4261 ctcgaagtgc tggtcatgaa ggatgacctg atcgatgtcg aatcccaggc cgtgctggca
     4321 aggcgttacg acccgatcac cccggcgctg ctcgaacaga tcgggaaggc gggtttcagc
     4381 aaaatcgagg tggtcgatgt ctcgttcgac ggcggcgtgc tgatcaagac gttgcgcgag
     4441 gatgccaagg cgaacatcta ttccgaagag gacgccctca aggacatcta caagcggctg
     4501 cgccccggcg atccggccac ggcgtccaac gccaaagccc tgctcaagcg cctctttttc
     4561 gagccccgcc ggtatgacct cggcaaggtc gggcggcaca agatcaacca gcgcctcggg
     4621 ctgtccggca aggttcccga caccttgcgc acactggatg agagcggcgt ggacgtggtt
     4681 gaggcggtcc ggctcctgct gaagatctac accggggaag aaacggtgga tgatatcgac
     4741 catctgggca gccgccgcgt ccgtacggcc ggcgagttgc tggagaacca gtgccgcgtg
     4801 ggtctggccc gcacagagcg gctgatccgc gagcgcatga cgctcttcga tcccgccagc
     4861 ggggtgctgg cgccggcccg tctggtgaac agcaaatcgc tctcggccgt ggttcaggat
     4921 ttcttcggac gcagccagtt gagccagttc atggaccaga ccaatccgtt gagcggcctg
     4981 gcccacaagc gccgcctctc ggccctcggc ccggggggtc tcagccgcga gcgcgccggg
     5041 ttcgatgtcc gcgacgtgca cgcgtcccac tatgggcgca tctgcccgat cgagacgccg
     5101 gaagggccga acatcggtct gatctcttcg ctgtgcatct actcgaagat caacgagtac
     5161 gggtttatcg agagccctta tcgcgtggtc cgtaacggca aggtcacgaa ggaggtgcag
     5221 tacctctccg cggacaagga agaggagttc gttatcgcgc aggccaattc ggagttgaag
     5281 tccgatggct cgtttgtgtc gcagcgtgtg acgtgccgct acaagacgga gtttgtggtg
     5341 gtggaccgct ccagcgtgca gtacatggat gtgtcgccca tgcaggtgat ttcgatcgcc
     5401 gcgggcctga ttccctttct ggagcatgat gacgcgaacc gtgcgctgat gggtgccaac
     5461 atgatgcgtc aggccgtgcc gctgttgcag tcggaaagcc cctttgtggc caccggcctc
     5521 gaggcgcgcg cggcttcgga ttcgtgcgtg acggtgttgg caacggaaga cggtgtggtg
     5581 gcatacgcct cggccacgga gatcatcgtg acgccggacg gcaagatgcc gcccaaaacc
     5641 aagaagggcg aagccatcaa agaggcgggc cgttattcgc tgcaaaagtt ccgccgctgc
     5701 aacgccggaa cctgcttcaa ccagaagccg gtcgtcgagg tcgggcagaa aatccggaaa
     5761 ggcgatgtct tggccgatgg tcccgcgacg tatggcggcg aactggcctt gggcaagaat
     5821 cttctggtgg ccttcatgcc gtggaccggt tacaactttg aagacgccat cttggtgagc
     5881 gagcggatcg tccgcgagga tatcttcacc tcgatccata tccaggagtt cgaggtgggc
     5941 gcgcgcgata ccaaactcgg ccctgaggag atcacccgcg acatcccgaa tgtgggggag
     6001 gaggcgctca agaacctcgg tcccgatggg gtggtgcgaa tcggtgcgga ggtgcggccc
     6061 ggggatatcc tcgtcggcaa aatcacgccc aagagcgaaa ccgagctggc gccggaagag
     6121 cgcctgctgc gcgcgatttt cggcgaaaag gcggcggatg tgcgtgacac gtccctgacc
     6181 gtcccgagcg gggtctacgg catcgtgatg gatgtcaagg tcacctcgca gcaggatgcg
     6241 caccgcccct tcctctcgtt gacggcgtcg tcggaagaga agagggaggt ccgcgatatc
     6301 gaggaagagc gcaaaaagaa gcaggccgcg ctggaggagg agctgaccac cgcgctgagc
     6361 aacatcctgt tgggcgagaa aatcccgctg gacgtgctgg atgtcgagaa cggcgagatc
     6421 atcattcccg ccaaccgcaa aatcaccaaa accctgctcg cgcggctcgc caaggcgcat
     6481 gaccatatcc agatcgagaa cagcccggtc cagcagaaga tcgaagagat catcaacggg
     6541 ttccgcccga aattcgcgga tctggccgaa cagagcagca acgatttcgg ggctgaactg
     6601 gacggcatca tcgatgaggg cggggtgatc aagcaggtgc gcgtgtacat cgccgagaaa
     6661 aagaacctct cggtgggtga caagctcgcc ggccgccacg ggaacaaagg cgttgtcgcc
     6721 aagatcttgc cggattgcga catgcccttc ctgccggacg gcacaccggt cgatatcgtg
     6781 ctcaatccgc tcggcgtgcc ttcgcgcatg aacctcggcc agttgttcga gacctatctc
     6841 ggtgtggcct gcaaagttct gggcttccat gcatcgagcc cggtttttga cggcgtgagc
     6901 gaagaggaga tcgatgcgtt gctgcgccag gcccgcaagg tgcaggagag cgagatcggc
     6961 ggatcggcct ggatctgcga ggatggcaag acggtcctgt atgacggccg cacgggcgag
     7021 ccgttcgatc agcgcgtggt ggtggggcat atctacatgc tgaaactgca ccatctggtt
     7081 accgacaaga tccacgcgcg cgccaccggg ccgtactcgc tggtgacgca gcagccgttg
     7141 ggcggcaaag cccagtacgg cggtcagcgt atgggcgaaa tggaggtctg ggcgctcgag
     7201 gcctacggcg tggcgtacgc gttgcaggaa ctgttgaccg tcaaatccga cgacgtcagc
     7261 gggcgcacac gcatttacga ggcgatcgtg aaaggacaga acacgctgga tgccggaatg
     7321 ccggagtcgt tctcggtgct gatgcacgag ttgcgtggac tgtgcctcga catgaaactg
     7381 ttgggcagtg ataccgctgg cccgaccccc gaactttcga gaatctaagg cgagacgagg
     7441 agacgcacaa tgaatcctta tgcatcacga gaaatgattg gcgggaccta cgatgcgaat
     7501 gcgcactatg atgccgtaag catcacgctg gcgtcaccgg aaacgatccg gtcgtggagt
     7561 cacggcgaag tccgcaaccc ggaaaccatc aactaccgga cctaccggcc ggagcgtgac
     7621 ggtctgttct gcgagcggat tttcggaccc acccgcgact gggagtgcaa ttgcggcaaa
     7681 tacaagcgca tcaagcacaa gggtgtggtt tgcgaccgat gcggtgtgga agtcacggtg
     7741 tcgcgcgtgc gccgtcagcg catgggccac atcgagctgg ccgtgccggt gtcgcacatc
     7801 tggttcttca aatgcagccc cagccggatc ggcctgattc tggacaaaac cgccaacgag
     7861 ttggaacggg tcctgtatta cgaagattac atggtcatcg atccgggcga gaagagcggt
     7921 ctggagaagg cgcagttaat cacggaatcc gagtatcacg actacgtgga aaaatacggc
     7981 gagagcttca ccgcgatgat gggcgcggag gcggtgcggc gtgtgctcaa cgatgtggac
     8041 ctcgacctgc tgatgcagac gttggaggag cagatcgaga acacccgcag caagcagacg
     8101 cgcaaaaaga tcaccaagcg catgaagatt gtggaaggct tccgcaccag cggcgcgcgg
     8161 ccggaatgga tgatcctcga ctgtctgccg gtcatcccgc cggagttgcg tccgctcgtg
     8221 ccgctggagg gtggacgctt cgcgacatcg gacctcaatg atctgtaccg ccgtgtgatc
     8281 aaccggaata accgcctgaa aaacctgctg tcgctcaaga cgccggatgt gatcatccgt
     8341 aacgagaagc gcatgctgca ggaggcggtg gacgcggtgt tcgataacgg ccgtcacggc
     8401 cgcgcggttg cgggcgcggg gaaccgtccg ctcaaatccc tcagcgacat gctgaagggc
     8461 aagaccggcc ggttccgtca gaacctgctc ggcaagcgcg tggactattc cggccgctcg
     8521 gtgatcgtcg tcggccccga actgaagatc actcagtgcg ggttgcccaa aaagatggcg
     8581 ttgaccctgt tcgagccgtt catcatccgc cgcctcaagg agaagggcat ctgccacacc
     8641 gtgcgcacgg cgcgcaagat gatcgagcgc catgacgagc aggtctggga tatcctggag
     8701 gaggtgacca aaggcaagac ggtgatgctc aaccgtgcgc ccacactgca ccgcctctcc
     8761 attcagagtt tcgagcccgt gctcatcgaa ggcgaggcga tccgcatcca tcccatggtc
     8821 tgcacggcgt tcaacgcgga ctttgacggc gaccagatgg ccgttcacgt gccgctgtcg
     8881 gtcgaggcgc agctggagag ccgcctgctg atgctgacgt caaactcgat cttctcgccc
     8941 gcttccggca agtcgatcat gacgcccaca caggacatcg ccctggggat ctactacttg
     9001 acgattccca gccagagcga caagctcgcg aacaaaatcg ccggcaaagt gccggccggg
     9061 gcagagcggt tgccgctctt caacactacg gcagaagtcg attccgcatt ggcggagggg
     9121 gcgatcacgt atcattcccg catccggttg cgcaatcccg atttcggaac gcaggatcgt
     9181 cgcttcggta atcccgagcc gagcgtgatc gaaaccacgg ccggccgcgt gattttcaac
     9241 gaggtctggc cggacgaact cggtttcgtg aacgataagg tcgataagaa caaactgggg
     9301 gacctgattc tccactgtca tcaggttgcg gggcacgccc gcacggtcaa agcgctggat
     9361 gaactcaaga ccctcgggtt caagcacgcc tgtctggccg gtgtctccat ggggctgaaa
     9421 gatatgatcc gtccgaagaa taaggatgag atcatcgccc aagcccacag cgaggtcgaa
     9481 aaggtggagg cgcaattcca gaccggtgtc atcaccgacg gggaacgcca caacaagatc
     9541 atcgacatct ggaccgccgc caccgaaaag atttcggacg agctgtacaa ggcgatcgac
     9601 cgcaacgtga ccgaaagcgt gtccgagccg aacgagacca accccgtgtt catgatggtg
     9661 gactccaagg cccgcggctc caaactgcaa atccgccagc tcgcgggcat gcgcggcttg
     9721 atggccaacc cgtccggcga gatcatcgaa cgccccatca cggcttcgtt ccgtgaaggg
     9781 ctgagtgtgt tggagtactt catctccacg cacggcgcgc ggaaagggtt ggccgacacc
     9841 gcgctcaaga cggctgacgc cgggtatctg acccgcaagc tggtcgatgt gtcgcaggat
     9901 gtgatcatca cgattcagga ttgcaacacg gtgaacggca tcgaggtcga agcggtggag
     9961 gagggggacg agatgctgat tccgctgcgg acccgcgtgc tcgggcgcac ggcgttgtac
    10021 gatatccgcc atccggccac catggaactg ctggttgcgg cgaacgccga aatcgacgag
    10081 gccgcctgca aggctctgga ggatgccggc atcgaacgcg tgtggatccg cagcgggctg
    10141 acgtgcgacg cgccccacgg gatgtgcgcg aaatgctacg gccgcgatct ctcctccggt
    10201 aaactcgtgg agatcggcac tgcggtgggc atcatcgcgg cccagtcgat cggtgagccc
    10261 ggaacgcagc ttacgatgcg gaccttccac atcggcggca cggcctcgac ggtttccaag
    10321 attccggaaa tcgtgctgaa gaacgccggg ttcatcaagt atgtggatct gcgcatcgta
    10381 cagaacgacg agggcaacac cgtcgtgctg aacaaaaacg gaacgatcga gatcgtggac
    10441 gcgaacggca acgagatcga cacctacaat ctgcagatgg gcacggtgtt gttggccgcc
    10501 gacggcgcgg cggtcaaggc cggccagcgt gtggccgtgt gggatcccca ttcggtgccg
    10561 atcctggcgg ataagcacgg cgtggtgtcg ttccaggatt tcgtggacga tgtgtcggtc
    10621 cgcagcgatc aggatgccga aaccggcgcc acgacactgt tggtgctcga caccaaggaa
    10681 gatttgcacc cgcagatcat tatcaccgat gccaaaaccg gtgagattct gggtttctac
    10741 cacattccgg tcggtgctgt cgtgatggtg aaagagggca cgaaagtctc gcccggcatg
    10801 ctgctcgcga aaacgccgcg taaggaggcg aaaacgcgcg acatcaccgg cggtctgccg
    10861 cgtgtggccg aactgttcga ggcccgccag cccaaggatg cggccgagat ctcccggatc
    10921 gagggcgtgg tcgatttcgg cgagcaccag cgcggcaaac gctgcctgat cgtgcgcgac
    10981 gagggcggcc acgaccagga acacctgatc ccgatgggca aacaggttgt cgtgttcaag
    11041 ggcgaccggg tcaagaaggg ccagcagctt accgagggtc cggtggtgcc gcaggagatt
    11101 ctggatgtct gcggcccgca ggaactgcaa cggtatctgg tcaacgaggt gcagcaggtc
    11161 taccgcctgc agggcgtgga aatcaacgac aagcacatcg agatcatcgt gcggcagatg
    11221 ctgcgtaagg tccgcatcac caatccgggc gacacggact tcctctgggg cgagcaggtt
    11281 tcgcgtcaga agttccttga ggtcaacgag caggtgttgg cttccggtaa gcgcgcggca
    11341 gaggcgcagc ccgccctgct gggcatcacc aaggcttcgc tcgagacgga cagcttcatt
    11401 tcggcggcct cgttccagga tacgacgcgt gtgctgaccg atgcggccac catgggccgt
    11461 gtcgatgagt tgcgcgggtt caaggaaaat gtgatccttg ggcacctgat tccgggaggg
    11521 accggcttcc cgctgcaccg ccacatcaag ctcgtgccgc tggccgatcc gatcagcgag
    11581 gaagagatgg ccgcgctgca ggcggacgcg caggcgcagt tcgacgaagt gttcaactga
    11641 ccgctgcggt cagaccggat ggcgggcggg acgcggcgcg tcccgcccgt cgtttgtttt
    11701 tccggtttac gggacgattg cggacgccgc gtcctgaaag gcgcgacgga tttcggaggc
    11761 gtcggcgttg gggaagtcgg cgcgttgcac catcaggatg taggcggccc cgcggaccgg
    11821 atcaatccat gcctgggtgc cgtacgcgcc gccgtgtccg aaggtgccgg gggagagttt
    11881 cgccgtcacg ccctgcggct ggcgcacaag accgcagccg aggctccacc ccatgccgtc
    11941 ggtgaaaccg gttttcaact cgccgctttg cagcgaggag aaggcggcaa ccgcttcggt
    12001 tgacaggatc cgccggtctc cggcggtgcc gcgcttgagc agcatccggc agaaacgccc
    12061 gtaatcggcg gccgtcgagt agaggccgcc attggccgca gggtagcggt ccgggccgcg
    12121 ccggaagtca tacagtgccc gcatatcggc ttcctccagc cgcccgtcga cctgccggta
    12181 cgctttcgca atgcgtgcgc actgggcggc atccggataa aaggtggtgt cgcgcatgcc
    12241 gagcgggtcg aagagacggg ccttcagaaa agcgggcagg ctttgttgcg agacgacctc
    12301 cacaatgcgg ccgagcgcgt tgatgcccga ctggctgtac tgccatttcg tgcccggctc
    12361 gaagcgcaga ggtttggaga cgatatgcgg catcagttcc gccagcgtgc gcgccgcaaa
    12421 cgcttccgct tgggtgggtt cggacaggcc cgaagtgtgc gtgagcaggt gtttcacggt
    12481 cggcgagaaa cccctgccgt ccgcggtttt caggttggcc agttcaggca ggtgggtcgc
    12541 ggccggcgcg tcgatcgaaa gcttgccctc gtcctgcaac atcaggatcg cgctggcgat
    12601 caccggtttg gtcatggagg cgatccagaa aatcgcatcc ggggtcatcg gttttttcgc
    12661 ggccagatca gcgtggccgc aggcatcgaa gtgaagagtc cggtctgccg tcaaaacgag
    12721 cgacaccgcg cccgccacct catgactgtc gagactgccg cgtagcgcct ccctaatggc
    12781 ggggcacacg ggttgctctg ccaccggcgt cagccgcacc gcgtgacctc cgttttcggc
    12841 catatccgcc gtgagcgtgt cggcggaggt cacgatccgt gtggcgcagg ccacgcgtgt
    12901 acggtccgaa ccgtccggtg cggcgtcgct gtatacgtgc gcggtgtagc gcacgttggg
    12961 ggagaggaac gaaagcggga ggtccaggct gcgccgctca agcgcattga tcgagccgat
    13021 gaaccaagtc tcgccgctgc ggcgggcgat ggaggcgtag gcgccgatcc gggcatgaag
    13081 cacgcgggtg tcgtcccaca cggtggggat ctgacgccaa aaatcgagtt cgggttcggc
    13141 ctgatacatc gaaggcctgt cataccagaa gaggaactgc caggggctga aggtgaccgc
    13201 cgcgagcgcg agttggtggg cgcgggtgtt tttcacgcgg ttgttgtacc agcagggcgt
    13261 gtagtcgccg ggaccgcaca ggtagcgcgt gaagggcagg gcacagttgt gcgccgcgtc
    13321 cggcatctcc tcgttgcccc ggatgccttc gaccgtcatg acgttcggcc aggtgcgctg
    13381 gttgccggtg aggcggtatt cgtcgtggat gtcgatcatg atgcgggcct cgcccgcctt
    13441 gcggatggcc tcgtgcagcc aggtcgtcca ggtctggctg ccaacctgga cgaacccgta
    13501 tttcatcccg acgacccccc aagaggcgta aagcggcagc agctcgtcga ggcggcgctc
    13561 gagttcgcgg cggttgacat ataggatgac acccacgtcg cgggccttgg cataacggat
    13621 gacttcgggc agatccagcg ggcccttgga acgtttgggg tcgaggttga cgccgcgcgc
    13681 gtccgcagcg tcatcgtact cgtgaccgta ccagcccgcg tcgaactcaa cgaactggag
    13741 gttcatcgtt ttgcagaaat cgacgcaggc tttcccgcct gcggtggtga gcgaggtctc
    13801 gcggatcacc ttgcccggcc ggatccagga aaggtcggcg atgcgggagg gttcgttcag
    13861 gttcagcagc aggtcgtttt gttccagcag gcgcgcggca ttctccgcca ccatgatgaa
    13921 gcgccagggc gtcgcgccgg gcagcggcaa cgacgcctga ccgtcaagcc gcgcggtgag
    13981 cgcatgcggc gtgccggcca gcggcgcgaa cttcatgcgc gcgaaggtga ggaggcccgc
    14041 ctcaccgatc gcggccacgg gaccttcagc cgtctccacg gtcagcggcc gctcgcaccc
    14101 gttccggatt tcgctcaggc gcgcgggctt gtacgccgcc tgggcagagt aaaccggcca
    14161 agcgcgatga tccccggtaa aaatgaattc ggtgttttcc tttgcgaaaa ggagttgtgt
    14221 gagtgactgt tgcggaaaca catagcggaa ggccagcccg gcgtcatagg cgcggcactc
    14281 gagatgcaga gtcaggtcgt tcgcagcgcg gtttcggaga atgagcgtca gcgcgttata
    14341 ggcgtcggtt acctgtgcgc gctcgccgaa ggggtttttc caagtctggt gtatggtggc
    14401 gcgcttcgcc gaaaccaagg catacccgag agtgaggtcc gtgcggtcaa cagtggtgaa
    14461 gcccagcttg gaaggcagaa tcaccgggac gccgccgaag gtcacgcgat acgtgagagt
    14521 tccctccggg gagagcgcga acacggcttg cagcttttgc ccgggcgact ccagagcgag
    14581 tccctcggcc cgcacaagcg cggcagtgaa aacaacggcg gagagcgcga tgacggcgcg
    14641 aagatatcgg atcatttcct tcctctcttt ccaaacggtt tggcagcaga ataatggatt
    14701 caggagccaa agggaataag caataaaaaa cggttgcaaa gttcccgcaa aaaggagtat
    14761 gtttatcccg atgattggct ggattgattg attgaaccga caaggacaac gcatgaggaa
    14821 aagaccggac cccacagact gatttgattt ttgcgctttc gcgcaaaacg ctccctacga
    14881 aaagccgtca aatctttggg aggagcggga tgcacgggga cgcgcccgat cgggcgcggc
    14941 ctcgtcgccg ggcttctccc aaggtgcttg acggtacctc gtaggtggca cggaacgggg
    15001 tccgcgcctt ttccctgccg ggaaaccggg ggaggaaacg gaaaatgaaa cggaaacgcg
    15061 ccgcatacgc atacgcgacg atcgcctcgg cggcagcgct ggcggcatcc gccgccacgc
    15121 tggacgtgcc gcgcctgccg gagcccgcgt tcgccgaccg cgaggcgtcg gcggcgcacg
    15181 ccctgcccgc gggccggaag gacggtctcc gcacgttccg gctcgagctg tccttcgggg
    15241 ccacgccctc caacaacgtg caggcggcgt tcgggcggga cggcgtcccg gccgacggcg
    15301 cgctcgacgc gggggagacg gacctcatca tcgggtggga ccggggggag tggttcatcg
    15361 cgccgcgcgg gctgaggggg cggcacgccg cgcccgccgc cacgtccaac ggcctccaga
    15421 ggctcaccgc cctcatccgc gtcaccggcg cgggcgtctg cctgcccccg tccttcgccg
    15481 accgcgacgg cgcggtcgtc ttcgcgggcc tccccgccga cccctttccg gaatggctgc
    15541 gtcccgacgc gtgggacacc ctgcgcgtga ccgcgcgcgg cgcggacgcg ccgctcgaat
    15601 ccgtaagggc cgtgttcgcc gcggacggcg cgctcctgaa actcagatga agagggaggc
    15661 gcggcaatgg cacccgaaac acttttcatt ttcctcggca ccgcttccgc gctctggctc
    15721 gcggccagag cgcacgaggc ccgcaagggc aacggccgcg gcagtctctt ccgcgccctg
    15781 cccggcgtcg accgggcggt tttcctgatt ctcgccgcgg ccctctgcct ccgcgccgtc
    15841 ccgaaacccg tcccgccctc cggcgcgccc gcgcccgccg cccaacgccg ggggcccccg
    15901 gcggggagcc cgccgccccc ggcggcgtcc ccctccggcg gcgccgcccc cggcgccctc
    15961 acccccgccc agtacgccgc gggcttcgcg ctgacgcgcg tgtccaccaa ccccgccgcg
    16021 tggctggcgg ccccctccaa cgcggtcgtc cacgcgccgt gggccgtgta cggcgtcgcc
    16081 gaggacatct tctggctgca gacaaacggc tggtccttca ccctcggcac caaccgggtc
    16141 gagggcctgt acgtctccag ctcggggctg gtgtcgttcc gggacccgtt gtccacgccg
    16201 aaaccctcct ccgtgcccga gaacgccagc ctgctcgcgc cgctgcacgg cagcttcggc
    16261 accgtgccgc cgcaggggcg cttctggcac gccgtgaccg cctcgaacag cctgctgctg
    16321 acctgggaga acctctacgc cgggcgcgag accaactcgc ccgtctcctt ccagtccgaa
    16381 ctcttcaaaa acggcgactt cgtttaccgg tacgccttca ccgacgcgcc ggcgctgacc
    16441 aacttcttca tcggcgcgcg gcacaaccac ggcggcgaaa ccttcgcccc cggcgacccc
    16501 tccgcgcccg ccggcggcct cgagctgcgc tggcgggcct tcggcatgct cgacccgtcc
    16561 gtcagcgacc atgacgggga cggcatcccg acatacgacg agcttttcat acacggcacc
    16621 gacccgtcca tggcggacac ggacctcgac gggctctccg acggcgccga gatcgcgctc
    16681 ggctcctgcc cgctcacgcg cgacacggac ggcgacggca tccccgacgg cgccgacccg
    16741 gacccgctca acgcgacccc cgccgacgac ctcgacggcg acggcctgcc cgacgcctgg
    16801 gagaaccact ggttcggctc gaccaacgcg acggacggcg cgggcgcgga caccgacggc
    16861 gacggcttca gcgaccgcgc gggcctgctc acgggcacgg accccgcctt cgccgcgtgc
    16921 gcggcctcct cctcgcacgg cgcgcacgcc ttcgcgtgga cggccctccc gggcgcgacc
    16981 aattacgcgg tctgcgtctc ccggggcggg gcggcggtct ggtcgtgcgc gaccaacgcc
    17041 aacgccctct ccgcctccgg cgacttctcc tccgccgtcc acacgctcac ggtcacggcg
    17101 caccgggacg gcgcggggcc ccgcgccgcg tccgtctcct tccggcagcc ctcgcgcccg
    17161 aacctgacgg tctggaagat cgccgacccc ttcgcgctcg aggcccctcc gggcctcacg
    17221 aacgtcctct gccggacctt cccggcggcc cgcgacggcg gctggcagca gtacttcgtt
    17281 tcgtccgggc acgactcggc gggcgcgtgg tccctcgagg ggctgcggct cgagtggtcc
    17341 gacagctgcg gcgcgtccgg gtcggcgtcg gcctcgccgc ccggcgacag cctgcgcctc
    17401 gcgctctccc cggacggccc cgggaccctg accgttcgga tcgtcccgga cggctcgggc
    17461 ggggccgcgc gctccccgaa acccctctac ctgctccgct ggtcgcccgc ggtcgccttc
    17521 tccccgacgg ggaccgtgtc cgccgtgacg ggggacgggg ccgtggcggc ggcggtgtcc
    17581 gaccggggcg gggggccggg cccggcgtcg ttcacggtcg accggtccgg gcgtccgcac
    17641 aacgcgcccc ccggcccgga cgagctcgcc cggcagtcga gccccttcgc gccgggctcc
    17701 ggcgcgtcgt tcgagggcgc ccacggcgcg gacggcgccc tgtccggcgg cacggtcctg
    17761 tccggcgcgc ccggcctctt cggccttccc ggcggcgggc tcccccccgg ccggcccttc
    17821 cccggctccg gcggcgccct ccagcccccg cccccggccg cgccctccgc ccccgccgcg
    17881 ctgctcctcc tctcccccgt cgtggcggtc gacgccgaca ggggccgctg ctcctgctcc
    17941 ggggcggggg gggagcgggc cctccgggaa tggccgctcg gctccgggtg cctgcgggag
    18001 atgtggcgga gcggcggcgg gtgcggggag tacctctccg agaccggcgg cttcacggtg
    18061 acggcggggt cggacgaggt cgacgccctc gtcacgttcc tcgtcaacgg cgaggagtgc
    18121 ccgtcccgct cgcgcgcgtg gcaggggggc gagacctccg ccgaggtctc cgtcacctac
    18181 ggcggcgcga ccctctggtc ggcctccgcc gcgagggagc ccgcctcgtt cgacggctgc
    18241 ggggagggga cggggtggga gggggaggac gccggcggcg gggagtgcgg ctgcggctgc
    18301 gcggacggcg gctgcgactc cctcgagggc gacgagatcg ggtcggtgcg cttcagggtg
    18361 ccgctcgggg agacggggcg cgggcggctc gcgggcttcg tctggttcga gatcacggag
    18421 ccgcgggccg tgacgcccgc gctcttcgag gtcgcgggcg gcgcctccgt gctctgcgcc
    18481 agcaacaacc ccggcggcgc gctcagcgac gtctcctgcc cggagccggg cggacgccgg
    18541 gtcgcggtcg cccccgcccc cggcggggcg gccctcgcgg tctacgccgg acacggcgac
    18601 gcggacccct cgcactcctg ggagatcacc aacccgtccg gcgccggcac cgtccgggtc
    18661 gtccggaggg acggggccgg gaacatcacg cgcgacgcgc tctacacgca cgccgaccac
    18721 gggtgggggg cctcctggac gcgggaggac ctcctcaccg ggctgacgga aagcgtcctt
    18781 taccatgacc cgcccgcggc cggcgggcac gtctgtgaga tgcgcacggc cacggactcg
    18841 ggcggcgcgc gcgtcctgtc ggcgaccgag gtttaccggg ccgaggtcgg ctccgggccc
    18901 gccgccgtca cgcgcgagac caggcggtgg gagtgggacg cggcgctcgg cagcgggatc
    18961 gaggtctcca tgacgtggtg ggaggacagc ctgaacccgc tccgcaacgg gaagcccaag
    19021 ttccggcagg gcggcggcgg ggagtgggag taccgcgcct tcgacgcccg gggccgcgag
    19081 gtcctgcggg cggggccgct ggacggctcc ggccccccgc ccttcgcctc cgaccccgcg
    19141 cccttcgccc tttccggcgt ccccgcgcgc cccccgttcg acgccctcgt caccgaaacc
    19201 tcgtacgagc ccctcccggg cgactccggc gcccccgggg actcgcgcga gccgcgccgg
    19261 atatccgcct acgccgtgag gggcggggtc ccggcccccg tctcccgcga gtggcgcgtc
    19321 ttcacgcgcg gcgcgtccgg cgggctcccc gccgtcaccc ggcgcgtcat ccgcgccgcc
    19381 tcgccctcct cccccccgga ggaccccgcc aacgccgtct ccgtgacggt ctccgccgcg
    19441 ggggacgacc cctccgtccc cgagcccctg cgcggcaggc cgctcctcga ggtcccggaa
    19501 gacggcgcga cgaactccac ctcctacgcc ttcggcggct acgaccccgg gagccgcacc
    19561 ttcaccgaac accccgacgg cgccttcctc cgcgccgtct cgtccgcggg ctcccggccc
    19621 ctcgcctcgg cggaggtctc cgacgccgcg ttcggccgcg tcctgctcag ggaaacccgc
    19681 ctcgccgccg ggggcgcgct cctctcgtgg gaggcgcgga cgcacgacgc cctggggcgg
    19741 catctcatgt ccgccttctc cgacggcacg gccgaaacca gcctctgggg ctgctgcggc
    19801 ctcgacggcg agaccggccg cgacggcgcg cgccgcgact tcctcgcggt gcccggcctg
    19861 ccgctctggt ccgccgaggc cggggcctcg gccggcgccc tgcccggcgc ggacggacgc
    19921 catcccgtca ccgagacctt ccgcgacgcg ctcgggcgcg tgaccaacac cgtccgccgc
    19981 gtctggctca acgggatgcg cgaccccgcc tccgcccccc tcgagacccg cgtcgaatac
    20041 ccgcacggct ccggccgcct ccgcgtcacc accggcccct ccggcgtccg gaccgtcacg
    20101 cgcaggtatt tcggcggcag cggcgagacc gaagaaaccg tctcgccggg cgtcaccagc
    20161 cgcgtcaccc gcatacgcgg cggcgcgacg gtcgccgaga cgttccggga cggcaaacgg
    20221 acccgcgaga cccgctcggc ctcccacggc gcggacggcc tccgcgtcga gaccgtggtc
    20281 acggagtcat cggacggccc cgccgtgacc aactccgtca cggcctacga cttcctcggc
    20341 cgcgtcgtgt ccgtcgccac gccctccggc gtcgcctcca acttctacga cggcccgtcg
    20401 ggccgcgtgt cccgcagaac cgagaccggg aaacccgaca cgctctacga atacgacgcc
    20461 gggggcgcgg gcgtccccgc ccgcaccgcc ctggacgtcg acggcgacgg ccggatatcc
    20521 tatgcgggcc ccgaccgcat caccgccacg cacgaaacct atgaacagga cgcctcgaac
    20581 gtctggtggc gcgtgacctc gcaagccgta tgggcggaaa cgaactccgc cgcctgcgtc
    20641 acatcctccg tcatccgcgt ccgcctgacc gggctcggca cgccagcgcc ggacgcgctt
    20701 gaactgtccg gcggcgcgat cctcaccgcg cagaccgaga cgctcgaccg gcgcggcaac
    20761 gtcacccgcg tctcgaccta caccgacgcg ggggcggcgg ccgtctggac ggtcacggag
    20821 acgcccggct cggtccagcc cgcgatccag aagacggtcg ccgggcgcac cgtctcgatg
    20881 gtgtcatcca cggctgtgac gaacgcctac acatacgacg gcttcgggcg gcaggtctcc
    20941 gccaccgacg gcaggggcaa cacgaccgtg accgcctaca atgaactcgg gcaggtggcc
    21001 tacaccgtgg acgcggcggg caaccgcacg tcgtacgcgt atgacgggct cgggcggaag
    21061 atcgccgtca ccgacccgct cggcaacaca acgcacaccg cgtacgacag cgcgggcaac
    21121 gtcaccgcgc aatggggcgc ggcctacccc gtggcctacg aatacgacac cgccggacgc
    21181 agaatcgcca tggccaccac ccgcgacgac acgttcgatt tttcttcggt caccaactcc
    21241 tcaatcctaa atcctaactc ctccctcgac gtgacgcgct ggctgtacga caacgccaca
    21301 ggattattaa ctaacaaggt ctacgccgac ggctcgcgtg tccgttacac ttacacggaa
    21361 ggcggccttc tcgcaacccg cgtgtggtcg cgcggcgtga cgaccgctta ttcctacgac
    21421 gcgcttggtc agttgatcaa catcaattac agcgacgcca cgccggacgt cgcgttcgct
    21481 tatgaccgca tgggcaacat ggtttccgtc acggacgcga gcggaatccg cgtgttcacc
    21541 catgatcctg acgggaaaat gctttccgac accttgtcag cctcggggca agaatttgca
    21601 ttgttggaat cgcac
//
LOCUS       DAOSRB010000024        20764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2754_length_20764_cov_5.891545, whole genome shotgun sequence.
ACCESSION   DAOSRB010000024 DAOSRB010000000
VERSION     DAOSRB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20764
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2754_length_20764_cov_5.891545"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..2241
                     /locus_tag="PLZ74_02495"
     CDS             118..2241
                     /locus_tag="PLZ74_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97223.1"
                     /translation="MRYSKAVWAITAVVAVARSVFGVNWKSEVTSGAWDNAANWEGGV
                     VPDAVTPASIENSGVDYSITLNAAGTTCATNTVIGNAEGSTVFTIHSPLSFFDFTSSS
                     PKYYYKQNFGAKTIIGAGGAFNFLASTSDGLSSDQGLFITKSEFAVDGGKVDLRMNGH
                     VALSGTAQQPATLTITNGGSFAWHSSDGYGQFKVPENAKVRVHDGSLAFSQNRNHYHT
                     APFSMTGGEMVFSGTSVFSNSYVSAEGYTSVALEIRSGKMEFHDEAVCVVQSKAGVHS
                     DFLLIPSDAGKRVELTVTDQACFGVGVEPYLMTVGEWTPGGIAVAEFSSTEQCNLGRT
                     TAVGAYAGYGELNLRSGLIVLGKTYGLMVGTTDNQALNRARGDNAAHQPADAATWAPT
                     GLVRVAGANLYLSTPFVDTGYGCKGLAGFRIGDGVYARYNNKAVPAPAVGTLELSSGS
                     ITNAQGYWSIGAGDGYGRFVQTGGTLLKCDPDYPTYGGNQTMVVGFVGGRGEWVMSNG
                     TARVIAPMMIGGAAPDDFLFADINTGWCPFSSHDAVGTLTIACADKEKPCSLEVIKAN
                     TGNTDVQGVWLGRDGTGTLEMIGSGGSLKTPYLILTNGYENASGELLTHGKAKLRFVL
                     DEDGIASLTVSTKMAIADNAEIEVDGRAYTGETKKVKLVNVTGSRSGSFRPENIRVKG
                     GVLIQDEDRHLYFRHMKTGLSVMLR"
     gene            2257..5280
                     /locus_tag="PLZ74_02500"
     CDS             2257..5280
                     /locus_tag="PLZ74_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOR97224.1"
                     /translation="MKYCAMMAVMAAVGSALAGGNLLTNGDFEGGRLTGWGNCASPGG
                     STMSVTRDAHAGGYALEYTHNGIGNQAFCRNFVKVVPGAVYTIRGYLKGDAEKINIRL
                     FNYAEGMSYVGETDIYTATKLEDWTYFEKAFSAPEDIKSVGVGLLLVKGRAYLDDVEV
                     LEGNQIAEEPNVLWNSSFKLSYHDNGMPEFWNTGGGHSFQTFQQGTHEGKFFRMADDV
                     ASPVEGARVLYVNKVGRTYLRPGPLAGGTDYTFSVYARAVTGEAKMNLILRGMRPGKK
                     AFTVGSEWQRLIFTAHLDRAVAEATATLEYNRNELYLAAPMLHAGKTALPWKPSPVDA
                     TFPDLFAGNQVPPPPAKIETIALPRGGDWSAAVKIDRFYVHGDYGKHVDGRYQAAAYR
                     DGDVLKVAFEMASGKKPPVKSKPHGPLVTGNEAVELFVSPQANGAGALHFMGGRNGAY
                     ACQDNGNGQAEINEGWAFAASETAQGWRGIFEIPLSLFTQPGGEVWRMNFAVTEFDGV
                     KPLYFSAIRAGFHSAPDFFRVTGLPRGSKVEPPAVFVEYDFYTTEKTARFRLRNAKAP
                     VEMEVTGASVPLAQDGHVDHVTLTLSGNEGEIEIGAFADGYYEVVVKAANGEGRSHFR
                     KFADEKHYTRINRFNETINHDGQPFFPIIFTAWMGGGRNDKIPTDWHIGELKKRNFNT
                     VMIPNELYVLWDTRNSSEMRRTIAKKFTDAGFKLLIWSACGNLKEEGMMKAVVDYRKR
                     HIDELAEFKKDILGWYYLDEIGANWEKSFGVKLEDYPKGYRAIKDYDPARLHFINWNH
                     TGCTVGTKYFAEDGATDIYSLDCYTYSCKPNFGSLSAFEIGTSTLRSRMDDTHLPGIE
                     WLQTYAYQEGIREPHQLEYRNNVYVALVNNVTGYMHFIDVPEHKELWEGMGEAYGTAL
                     KWLGMTVEPGSRLVRRGRQGQISYALWERGSGGHAFIAANTLYAPQTEAIVFTVELGS
                     SVETVGGEGALESNGRSLSVALPTAGSAAWFITGR"
     gene            5432..8146
                     /locus_tag="PLZ74_02505"
     CDS             5432..8146
                     /locus_tag="PLZ74_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97225.1"
                     /translation="MGRQNVIRNVRGITLVSCGLALMEMAFGAPVLTGDTSKPAASIF
                     YPKDRVSLYFHVSGLAAFQKDITLKLTVENEDGKVFWGRDCPVKPDANGQDEVIVTDA
                     PGGRLGFYRLRARLSDGTLLTTPWATRQPGQLTWAVVPAPEEREPVDVSYLRFFNSGD
                     PDLVGRMAVDRVGPTNPKIGPFGITSQWRHLEAHRPGEYADDPEADRIPLPYAVGNLY
                     THSTAWGNDLLATHFKMQGHADGHPPALTPAGEKAWEAFVKTFARNFRRQYPTVPRVY
                     EFTAEYYAPDNGSRTLKDIVRMYEVGYKALKSEDPEGLVAGPGFGYAAKDYHQLEFLK
                     AGLARWLDVITEHSYYTYPAEPNGIVDAIRTSRQLVRQYAGRDLAFYSTEFGYATEDF
                     EPRERDQLRHILRVSLILFGEGFKALSAFVPSDYRLEPGFGYTYNLALIKDVDRKDDW
                     FHNKTSPKPIYPAYAALTHLLVNSETLGDVPFLGETAWGYVFRKANTGRIILALWDWS
                     GTPRAVKLPAGVPVVDVVDCMGNSRTVKTVDGELSLTLTDVPQYVTGVSPDVWTNPMG
                     ERAKLAAAFQARQKAARSARGVEITGVSSACMDDGRAAVDVTLANLRAETNEGVVRVR
                     VKGNPDGRASAPYRLSPEQTVSVKVPMAADFVGAALMNEEVEVAVEQKAGPRVECGEN
                     VNFLFAPYRPKVAVDGIFDDWKDVSISRFDPARQLTNGKRYYKGPDDCTATLSVCWDD
                     RFLKFLFDVVDDQYVPPPASKTVWSRDSVQMAFSGVYHLIKTGNMLQDKLNEAATECT
                     WGLTEKGPYAYRHVSFDNAICPRGPLDLSTHPFHASIALCTNGKSRIRYEIAVPWRDM
                     RVAEPKVGVRVGWACTVNDWDPGKPAYSAFGAFNLKIPDQFGAIVLTP"
     gene            8153..9475
                     /locus_tag="PLZ74_02510"
     CDS             8153..9475
                     /locus_tag="PLZ74_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97226.1"
                     /translation="MMFATGLFVLAATLQFNGIMGQSAVPGDPPLTFTGVRALTEGPG
                     GRLLLTGDDGVLYALVDGEVVSLGVRVPAAHLAWDGRTLWVLGSDAVWTVDEECPGCF
                     AAHKAFDVPGLHFDQAAVRPELADHPFAGKGKFFRWDRGRNVLVAHDAAGRECGTVFA
                     FPRRKGDSALVALGFLPGTEDLVASSYYPDVRLYRFRVDGSQVTGNGWPANRGYGLLR
                     RFKGRLYHMGTDVLPLGENLLGARAMRVGHEAHHRGWATDGVRDYLATSQGLYVREPG
                     ETRFRTRRGGIRPLTALAVNAPYVFLSMGGTVRWLYLDEDATGVFASSDDDNLRVANA
                     WIYPPLDMVPEGANLLVAGGEAGLYCFHPFLPPGASPRDHWEKRAPGPCPRVPGCRET
                     FPGVEAAIVAAGAALPEGIEPGLVARQGKWLVVEDRKNFRLVRFKVLN"
     gene            9506..11281
                     /locus_tag="PLZ74_02515"
     CDS             9506..11281
                     /locus_tag="PLZ74_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97227.1"
                     /translation="MMGNKTLIAALLAGCGTVVFATSFPNADGSGDIASPTAWGGVLP
                     TDQAQFQTSDVTNTASANVTFAGVEVGAKNVVFDFSQTPARTITLSDHFRPLNYTGAS
                     YTLRGGTWNLSGKNFHLYHNWGQGSECTVTLENGCNVTNVGWTLLNFSTLKCHLIIRD
                     QASLTVNTYDSNSGHGNDEPCLEVLSGGRLSVPATGSTLRIGDGTTLVSGAGSRIESY
                     GGAGDFTLGNTYHNTLIRITDGGAFDAASRRMNLNLNDATNTTIVVDDDGTFQCGEVY
                     PNGRAFNIQIGERGQMTTGRWYIKGNDHSVRVLNGTLKGSRWIGEAASGASVTNFSVA
                     VIGSDAQVDIAGGDALFMGLSDSSILLEGASWSCPVTYWKSRESSNCTFRLTQQAAFT
                     TTGHFWGTAYDGGSASQTNCNGNVISVESGSTFQSAGFTFVGKGNRLIVTNATFRCPT
                     GSFNFGRSDRGSDANQLVLQGTTPRVEIASDAMHLRYGSTLRVEMPAEGYAADFVPIT
                     TAWSLSLDDSCHLVADVEAIRRHQRVNKTYVLARANGGVSIPESVLAEANAVAPDEWQ
                     FGVSEDNKSLTLRVMLLRGMAVMLK"
     gene            complement(11374..12354)
                     /locus_tag="PLZ74_02520"
     CDS             complement(11374..12354)
                     /locus_tag="PLZ74_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOR97228.1"
                     /translation="MIPELQIFLILCCFAAVAFFSATEAGVLSISHARLIHLVRAGMK
                     SAVLLNGYLADMQRFMGAVLVGTNLTNVILSTLSASLAQTCLPNSRTLQSVWAVTMAC
                     ILLFFCEYLPKLFFTTRPLRRTLMIIRLFYFVEKAISPLTALVLWLTKWLTPQKAGAQ
                     GQRFLMTREYIQHVVSDARNGARISAFERLMINRVLTLQSMKAEQVMTPLARVTKTVE
                     DAPLEECYRLVRESGHVRLPVFSADGSRCVGVLNVLSLLAQAPDPKRTLARDGMHPPY
                     FVRADECADNVLPLMRAYRQPMVLVRATQDNAVLGIITEENVLSALTGSL"
     gene            complement(12351..13670)
                     /locus_tag="PLZ74_02525"
     CDS             complement(12351..13670)
                     /locus_tag="PLZ74_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOR97229.1"
                     /translation="MNPIFFMLNGIVLLTLFALSAFFSGSETVLFSLSPIHVQRIRNR
                     NAEAAARLERLLANPSLVLSTLLVGNTLVNFAIATIGYLMFSRLTPQWGEVIAIPVVT
                     IALLLFGEVTPKRIAVLHAERLAPLCSRLLLFWLWLLRPFNLVLTAASRVFKHALRRE
                     RRALSNNELLTVVEVGEEQGVIDAEEASMVDGILRLSELMASDEMTPRVDMVGLDLDT
                     PVAQQVETVRKAQFRYLPVYVRTPDAIEGFLDATMFLLDPTHNARKATTPALFVPENV
                     TLDDLLVTFQRSGKHIACVLDEYGGTAGLITRGDILELITVPVARNGEERTPAIRNLQ
                     GDIWLVDGTVSLDELNHRLDLQLEADDADRIAGWITFHAKRLPQPGQVVEAQGCRVTV
                     RRLRKRRIDVVQLEVLERPEETPEDLQEDLQEVDDLPYDHPEEGELR"
     gene            complement(13667..14440)
                     /locus_tag="PLZ74_02530"
     CDS             complement(13667..14440)
                     /locus_tag="PLZ74_02530"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOR97230.1"
                     /translation="MKSTPGCVCLLCVILIGAVCRADDESRPATAAAALEENLTAAQI
                     LSACTQLIPTDRLLLQGTLTVRKLRGIKLAEEPFKLMLDWGARPPSAEVLLLDATGTS
                     LVERAVLTRPAGRPAQISLFRGPEQQPVDSPSYAGRVRGTDMTWMDLTLDFLWWTDVR
                     FDDVPRGQSRNGRDCDILVTVPPAPIPGCSAVRVWVDRQLRCLMQAEQLDPQGTPVRK
                     MWVQRVKKMDDRWMVRDMEIETLNSGHRTRLSVIDVSSP"
     gene            14632..15423
                     /gene="lgt"
                     /locus_tag="PLZ74_02535"
     CDS             14632..15423
                     /gene="lgt"
                     /locus_tag="PLZ74_02535"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOR97231.1"
                     /translation="MALGFLFGWRTAVLLCKRTGQDADQLTTLLTWLMISALLGARAA
                     YVLEHWHAEFADAPLMILRLDQGGLMFYGGFLAAALVLLLYARIHRQHVLSITDLVLT
                     VVPLGHAFGRVGCFLHGCCYGRVTTSRFGVAFPQGSPAWWEHVSAKPPLISQHATASL
                     PVIPTQLIEAGANALLFVVLFTLYPRCRTLRGLLTGCYLMAYATLRFGIEMLRGDPRL
                     AVGPFSISQAISLVLFALGLALAVYSPRMRLDVRIAQNTLPHMEQ"
     gene            complement(15384..16640)
                     /gene="thiC"
                     /locus_tag="PLZ74_02540"
                     /pseudo
     CDS             complement(15384..16640)
                     /gene="thiC"
                     /locus_tag="PLZ74_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010902492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            complement(16657..17526)
                     /gene="thiD"
                     /locus_tag="PLZ74_02545"
     CDS             complement(16657..17526)
                     /gene="thiD"
                     /locus_tag="PLZ74_02545"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOR97232.1"
                     /translation="MSDERNDECACLEPVRLPVALTIAGSDSGGNAGIQADLRAFHVF
                     GVHGCTVIAALTAQNPFGVRAVLTADAAFVAEQLDTVLDVYGITALKTGMLACPEVIE
                     AVADCLVCHNRIAKVIDPVMVATSGARLLAEDALEMLTSRLLPLATLLTPNLAETEVL
                     LGSDRTVLTLEAMQEAARELADRFGCAVLVKGGHADSPCAEDVLYDGAALHRVTTPRL
                     VSPASTHGTGCSLSAAIAASLALGRPLVEAVTEGKAYVYESIRTGAQVGEVATVLGTP
                     TRLPCDRVTVETV"
     gene            complement(17523..18482)
                     /locus_tag="PLZ74_02550"
     CDS             complement(17523..18482)
                     /locus_tag="PLZ74_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOR97233.1"
                     /translation="MGFAKQVGMWLAGISLASAVTGAEPTSATSQRFVRYRSGFNRDV
                     LSLQVLLDRRNLSCNCVDGMWGKRTQIALMTWQTLNGLPATGVPDAAVLEALGGDADV
                     LTRHTVTEADRAAIGPVPESWEERARLPSMTYETIQEKVAEQGHTSQRALARLNPEVA
                     WPNPPVGTEIVLPDCTTRVPSVKAGSVRIALSRMEVTVFDAAGKLIALFPCSIAREKR
                     HRPDGELTVKNIAPNPNYLYDPHLFDPKGPKRSKLVIPPGPNNPVGLAWVGLSLQGYG
                     IHGTPFPEQIGRAESKGCFRLANWNAVRFLNLVEPGLPVVVEE"
     gene            complement(18740..19519)
                     /locus_tag="PLZ74_02555"
     CDS             complement(18740..19519)
                     /locus_tag="PLZ74_02555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOR97234.1"
                     /translation="MITETYYDTHAHFTLTAAETVAVLSRAREAGVSRVVAVGGNAKL
                     NAGALAAAQAFPDAIRLALGFDRDQATEAEPEVFVETLRQLAETYPFAAVGETGLDFH
                     FHPETAEAQCALFAAQLRLADVWKRPVIVHTREADDATLRVLDETPWHGQGLRGVIHC
                     FTGDTRFAAQLLDRQMALSFSGIVTFRQAESVRASAAFVPDDRLLIETDSPYLAPVPM
                     RGQRNEPAYVTHVAACLAAVRKTRPERVAEITTRNARALFG"
     gene            complement(19564..20025)
                     /locus_tag="PLZ74_02560"
     CDS             complement(19564..20025)
                     /locus_tag="PLZ74_02560"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOR97235.1"
                     /translation="MSALSKLLQRTLNLRRKETERKELPRSARVGAWGEEQAAAYLSA
                     RGYTVLGRNVRPNRRDELDLVVRQGDTLVFVEVKTRHRETFARPARAVNPAKRHALNR
                     AAVAYLRKARFPDLYYRFDVIEVLGQPEEGEPLIRHLENAFSFERRFVFPV"
     gene            complement(20030..20617)
                     /locus_tag="PLZ74_02565"
     CDS             complement(20030..20617)
                     /locus_tag="PLZ74_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOR97236.1"
                     /translation="MSENVTNVSLVGVGGQGILLTSDILAKTAVLAGLDVKKSEIHGM
                     AQRGGSVISQVRFGKKVFSPIIPDGASNLLVSFERLEALRWRHLLAPDGKVLINSLDM
                     TPITVSSGQQVAVTDLDDRLRAEYPSALYVDALTIAKEMGNMRTMNMVIAGALSALLP
                     FEPEIWKQAMRERIPGALIELNLRAFDTGRQAISG"
BASE COUNT         3912 a         6137 c         6630 g         4085 t
ORIGIN      
        1 gtgacctttt gccttcccta caaaatgtgt gccaatcaat gggagtcgat gggttgccgg
       61 tctgccctat gggatggtag tgacccctgt aagacggtga tgagaggaag cagtgaaatg
      121 agatacagta aggcagtctg ggcgatcacc gcagtcgtag cggtggcgcg ttccgttttc
      181 ggcgtgaatt ggaagtcgga ggtgacaagc ggcgcgtggg ataacgcggc gaactgggag
      241 ggcggggtgg tgccggatgc ggtgacgcct gcgtcgatcg agaatagcgg cgtcgattat
      301 tcgatcacgt tgaacgcggc ggggacgacc tgcgcgacga atacggtgat cggcaatgcc
      361 gagggatcga cagttttcac gattcattcc ccgctcagtt tcttcgattt tacgagttcc
      421 tcgccgaagt attactacaa gcagaacttt ggcgcgaaga cgatcatcgg cgcgggcgga
      481 gcgttcaact tcctcgcctc cacgagtgac gggttgtcct ccgatcaagg actttttatc
      541 acgaagtccg agttcgccgt cgatggcggc aaggtggatc tccggatgaa cggccatgtc
      601 gcgctgagcg gcacggcgca gcaacctgcg acgttgacga tcacgaacgg cggctccttc
      661 gcgtggcact cgtcagacgg ctacggacag ttcaaggtgc cggagaatgc gaaggtgcgc
      721 gtccatgacg gttcgctggc gttctcccag aatcgaaatc actaccacac ggcaccgttt
      781 tcgatgaccg gcggcgagat ggtcttcagc ggcacgagtg tcttctcgaa ttcgtatgtg
      841 tccgccgagg gttatacgtc tgtcgcgctc gagatacgat ccggcaagat ggaattccac
      901 gacgaagcgg tctgcgtcgt gcaaagcaaa gcgggcgttc acagcgattt cctgctgatt
      961 ccgtccgatg cggggaagcg tgtcgaactg acggttacgg atcaggcgtg tttcggggtg
     1021 ggagtcgagc cgtatctgat gacggttggt gaatggacac cgggagggat cgccgtcgcc
     1081 gagttctctt cgacagagca gtgcaatctc ggacggacta cagccgtggg tgcctatgcg
     1141 ggctacggcg agctcaatct gcgctcgggt cttatcgtgc tggggaaaac ctacggtctt
     1201 atggtcggta cgacggataa tcaggcgctg aatcgtgccc gcggggacaa tgcggcacat
     1261 cagccggccg atgcggcgac ttgggcaccg acggggctgg tgcgggtcgc gggtgcaaat
     1321 ctttatctca gtacgccgtt tgtcgacacc ggttatggtt gcaaggggct tgcgggcttc
     1381 cgcatcggtg acggcgtcta cgcccggtac aacaacaagg ccgtgccggc cccggcggta
     1441 ggcacgctcg agctttcgtc cggctcaatc acgaatgccc agggttactg gtcgatcggc
     1501 gcgggcgacg gttacgggcg cttcgtgcag accggcggta cgcttctcaa gtgcgatccg
     1561 gattatccga cgtatggcgg gaaccagacg atggtcgtcg gctttgtggg cgggcgcggc
     1621 gaatgggtga tgtcgaacgg aacggctcgg gtcatcgcgc cgatgatgat cggcggcgcc
     1681 gcgccggacg atttcttgtt tgctgatatc aacacgggat ggtgtccgtt ctccagccac
     1741 gatgccgtcg gtacgctcac gatcgcgtgc gcggacaagg agaagccatg ctctctcgag
     1801 gtcattaagg ccaacaccgg caatacggat gttcagggtg tctggctcgg gcgcgacggt
     1861 accggcacgc tcgagatgat cggttccggc ggttcgctca agacgcccta tctcattctc
     1921 actaatggtt acgagaacgc gagcggcgaa cttttgaccc acggcaaggc gaaactgcgg
     1981 ttcgtgctcg acgaggacgg tatcgcttcg ctcaccgttt cgactaagat ggcgattgcg
     2041 gataacgcgg agatcgaagt cgacggccgc gcctataccg gcgagacgaa aaaggtgaag
     2101 ctcgtcaacg taaccggttc gcgcagcggt tccttccgtc ccgagaacat ccgggtcaag
     2161 ggcggcgtgc tcatccagga tgaggacagg catctctatt tccggcacat gaaaaccggc
     2221 ttgagcgtca tgcttcgtta aggatgcgtt cacaacatga aatactgcgc gatgatggcc
     2281 gtgatggcgg ctgtaggaag tgctctcgcg ggcggcaatc tgctgacgaa cggagatttc
     2341 gagggcggca ggctcacggg ctggggaaat tgcgcgagcc cgggcggcag cacgatgagc
     2401 gtcaccagag atgcccatgc cggggggtac gcgctcgagt acacgcataa tggcatcggc
     2461 aatcaggcgt tctgccgtaa tttcgtgaag gtcgtgccgg gcgcggtcta cacgatccgc
     2521 ggctatctga agggggatgc cgaaaagatc aatatccggc tcttcaatta tgccgagggg
     2581 atgagctacg tcggcgagac cgatatctac acagcgacga aactcgagga ctggacatat
     2641 ttcgagaagg cattttctgc gccggaggac atcaagtcgg ttggtgtcgg gcttctcctc
     2701 gtcaaagggc gcgcgtacct tgacgatgtc gaggtgcttg agggcaatca gatcgcggaa
     2761 gagccaaacg tcctctggaa ttcgtccttc aaactctcct atcatgacaa cggtatgccc
     2821 gagttttgga acaccggcgg tggccacagt ttccagacct tccagcaggg cacacatgaa
     2881 ggaaagttct tccgcatggc cgatgacgtc gcctcgccgg tggagggcgc gcgtgttctc
     2941 tacgtgaaca aggtcggccg cacatatctg aggccgggcc ccctcgcggg cggcacggac
     3001 tatacgtttt cggtctatgc gcgggccgtg acgggtgagg cgaagatgaa tctcatcctt
     3061 aggggcatga ggcccggcaa gaaggcgttc acggtcggtt ccgagtggca acgactcatt
     3121 ttcaccgcgc atctcgaccg tgcggtcgcg gaggcgacgg cgacgcttga atacaaccgg
     3181 aacgaactct atctcgccgc gcccatgttg cacgcgggaa agaccgcgct tccctggaag
     3241 ccttcgccgg tcgatgcgac ctttcccgat cttttcgcag gcaatcaggt tccgccgccg
     3301 ccggcgaaga tcgagacgat cgccctgccg cgcggtggcg actggtccgc ggccgtgaag
     3361 atcgaccgct tttacgtcca cggcgattat ggaaaacacg tagatgggcg ctatcaagcc
     3421 gcagcgtacc gggatggaga tgtcctgaag gttgccttcg agatggcgag cgggaaaaag
     3481 cctccggtaa agagcaagcc gcacgggccg ctcgtcaccg gcaacgaggc cgtcgagctt
     3541 ttcgtttcgc cgcaggccaa cggcgccgga gcacttcatt tcatgggtgg gcgcaacggt
     3601 gcgtatgcgt gtcaggacaa cggtaacggg caggctgaga tcaacgaggg atgggcgttt
     3661 gccgcgagtg agaccgcgca gggctggcgc ggtatcttcg agatacccct ttcgctcttc
     3721 acgcaacctg gcggcgaggt ctggcgcatg aatttcgcgg tgaccgagtt tgacggcgta
     3781 aagccccttt atttcagtgc catcagggcc ggcttccatt ccgcgcccga tttcttccgc
     3841 gtgaccgggc tgccgcgagg cagcaaggta gagccgcctg cggtattcgt ggaatacgat
     3901 ttctacacga ccgagaagac agcgcggttt cggctgagga atgcgaaggc tccggtggag
     3961 atggaggtga caggggcaag cgtcccgctt gctcaagacg gtcacgtgga tcacgtgacg
     4021 ctgacccttt cgggaaatga gggggaaatc gaaatcggtg cattcgctga cggttattat
     4081 gaagtggtcg tcaaggcggc gaacggcgag ggaagaagcc acttccgcaa attcgccgat
     4141 gagaagcact atactcgcat taatcgcttc aacgaaacga tcaaccacga cggtcagccg
     4201 ttttttccga tcattttcac ggcatggatg ggcggcggac gcaacgacaa gatcccgact
     4261 gactggcata tcggggaatt aaagaaacgc aatttcaaca cggtcatgat cccgaacgag
     4321 ctctacgtct tgtgggacac gcgcaactcg tcggagatgc gcagaaccat cgcgaagaag
     4381 ttcaccgatg cgggcttcaa gcttctcatc tggtccgcgt gcggcaatct caaggaagaa
     4441 ggtatgatga aggccgtggt tgattaccgc aaacgtcata tcgacgaact tgccgagttc
     4501 aagaaggaca tcctcggctg gtattatctc gacgagatcg gcgccaactg ggaaaagagc
     4561 ttcggtgtaa agctcgagga ctacccgaag ggctaccgtg cgatcaagga ctatgatccc
     4621 gcgcggctcc atttcatcaa ctggaaccac accggctgca cggtcggcac gaagtatttc
     4681 gcggaagacg gagcgaccga catctattcg ctcgactgct acacctattc gtgcaagccg
     4741 aactttgggt cgctctccgc cttcgagatc ggcacctcga cgttgcggtc gcgtatggac
     4801 gacacgcatc tgccgggtat cgagtggttg cagacctatg cctatcagga gggcatccgc
     4861 gagccgcatc agcttgaata ccgcaacaac gtctatgtcg cgctcgtcaa caatgtgacc
     4921 ggctacatgc acttcatcga cgtgcccgag cacaaggaac tctgggaggg tatgggcgag
     4981 gcgtacggaa cggctctcaa atggcttggt atgacggtcg agccggggtc acggctcgtc
     5041 cgccgcggac gccagggcca gatttcctac gcgctctggg aacgcgggag cggggggcat
     5101 gctttcatag ccgccaacac gctctacgca ccgcagactg aggcgatcgt tttcacggtg
     5161 gaactcggtt cttccgtcga gacggtcggc ggcgagggtg ctttggaatc gaacggccgc
     5221 tcgctttccg ttgcgcttcc caccgccggc agcgcggctt ggttcatcac ggggaggtga
     5281 cgacaaggca cggcatggga tcttggaaat atgccgattt atgggattga cggttttcac
     5341 cttccctgca taataacggg tatcggtaac gggaatcgaa gtgtcccggc ctgtccgtct
     5401 actcaaggag atcgtcgtta aggaatatga attggggagg caaaacgtca tccgaaatgt
     5461 ccggggcatc acgctggtgt cgtgcggact ggccttgatg gaaatggctt tcggcgctcc
     5521 ggtcctgacc ggcgacacgt ccaagcccgc agcctcgatc ttttatccca aagaccgggt
     5581 gtcgctttat tttcacgttt cggggcttgc cgcctttcag aaggacatca cgctgaagtt
     5641 gacggtcgaa aacgaggacg gcaaggtctt ctgggggcgc gattgccccg tgaagcctga
     5701 tgccaacgga caggacgaag tgatcgtgac cgatgcgccc ggcggccgtc tcggcttcta
     5761 ccgtctgcgt gccagactct cggacgggac gttgctgacg acgccgtggg cgacgcgtca
     5821 gccgggtcaa ctgacctggg ccgtggttcc tgcgccggaa gagcgcgagc ccgtggacgt
     5881 ttcgtatctg cgtttcttca attcgggtga tcctgacctc gtgggccgca tggctgtgga
     5941 tcgggtcggc ccgacgaatc ctaagatcgg gccgttcggc ataacgtccc agtggcggca
     6001 tctggaggcg catcgtccgg gagaatatgc ggacgatccc gaagcggacc gcattccctt
     6061 gccgtatgcc gtcgggaacc tttacacgca ctcgacggcc tggggcaatg atttgttggc
     6121 gacgcatttc aagatgcagg ggcatgcgga cggccacccg ccggcgctga cgcctgcggg
     6181 cgaaaaggct tgggaggcct ttgtgaagac cttcgcacgt aatttccgcc gtcagtatcc
     6241 gaccgtgccg cgcgtctatg agttcacggc ggagtactat gcgccggaca acggcagccg
     6301 cacgctcaag gatatcgtgc gcatgtacga agtcggctac aaagccctca agagcgaaga
     6361 tccggagggg cttgtggcag ggccgggatt cggctatgcc gccaaggact accatcaact
     6421 ggaattcctc aaggcaggcc tggctcgctg gctggatgtg atcacggaac actcttacta
     6481 cacctatccg gctgagccga acggcatcgt cgatgccatt cgcacatcgc gtcagctggt
     6541 gaggcaatac gccgggcgcg accttgcttt ctacagcacg gagttcgggt atgccaccga
     6601 ggacttcgag ccgcgtgaac gggatcagtt gcggcacatt ctgcgcgtgt cgctgattct
     6661 cttcggcgag gggttcaagg cgctctcggc tttcgtgccg agcgactatc gcctcgagcc
     6721 ggggtttggc tacacgtata acctggcgct catcaaggat gtcgaccgga aagacgactg
     6781 gttccacaac aagacatcgc ctaagcccat ttatccggcc tatgcggctc tgacgcatct
     6841 gctggtcaac tccgagacgc tgggcgacgt gccgttcctt ggcgaaacgg cctggggata
     6901 cgtcttccgg aaggcgaaca cgggacgcat catccttgcg ctgtgggact ggagcggtac
     6961 gccgcgcgcg gtgaaactgc ccgcgggggt tccggtggtc gatgtggtgg actgcatggg
     7021 aaattcgcgt acggtgaaga ctgtggacgg cgaactgtcg ctcacgctga cggatgtgcc
     7081 gcagtatgtg acgggcgtgt cgccggatgt ctggacgaat ccgatgggtg aacgggcgaa
     7141 gcttgcagcg gccttccagg cgcgccagaa agcggcccgc agtgccaggg gcgtggaaat
     7201 caccggcgtc tcgtcggcct gcatggacga cggacgggcg gcggtggatg tgacgctcgc
     7261 caatctgcgg gcggagacga acgaaggcgt ggtgcgcgtg cgcgtgaaag ggaatccgga
     7321 cggtcgcgcg tcggcacctt accgtttgtc gcccgaacag acggtgtccg tgaaggttcc
     7381 tatggccgcc gatttcgtag gcgcggcgct gatgaacgag gaggtcgagg tggccgttga
     7441 acagaaggcg ggtccgcgcg tggaatgcgg ggagaacgtg aacttcctgt tcgcgccgta
     7501 tcgcccgaag gtggccgtgg acgggatttt cgacgactgg aaggacgtgt cgatcagccg
     7561 ttttgacccc gcgcggcaat tgacgaacgg caaacggtat tacaagggac cggatgattg
     7621 cacagcgacc ctgtccgtct gctgggatga ccgtttctta aagttcctgt tcgatgtggt
     7681 ggacgaccag tatgtgccgc cgccagccag caaaaccgtg tggagccgtg actccgtgca
     7741 gatggctttt tcgggcgtct accatctgat aaagacgggc aacatgctac aggacaagtt
     7801 gaacgaggcc gcgacggagt gcacctgggg actgacggaa aaaggcccgt atgcgtaccg
     7861 ccacgtatcc tttgacaacg cgatctgtcc gcgcggcccg ctggatctct cgacgcatcc
     7921 gttccatgcg tcgattgcgc tgtgtacgaa cgggaaatcc cgcatccgct acgagatcgc
     7981 cgtgccgtgg cgcgatatgc gggttgcgga gcctaaggtc ggtgtgcgtg tcggatgggc
     8041 ctgtacggtg aacgactggg atcccggcaa accggcctat tccgcgttcg gcgcgttcaa
     8101 cctgaaaatc cccgaccagt tcggtgccat cgtcttgact ccgtgaggaa aaatgatgtt
     8161 cgccaccggt ctattcgtct tagcggccac gctccagttc aacggcatca tgggccagtc
     8221 tgcggttccg ggagaccctc cgttgacgtt cacgggcgtg cgggcgctga cagaagggcc
     8281 gggcggccgc cttctgttga cgggcgacga cggggtgctc tatgcgctcg tcgacgggga
     8341 ggtcgtgtcc ttgggcgtga gggtgccggc tgcgcatctg gcgtgggacg gcaggacgct
     8401 gtgggtgctc ggctccgatg cggtgtggac ggtggatgag gagtgtcccg gctgctttgc
     8461 ggcgcacaag gcctttgacg ttcccggcct ccatttcgat caggcggccg ttcgtccgga
     8521 actggcggac catccgttcg ccgggaaggg gaagttcttc cgctgggatc gagggcgcaa
     8581 tgtgctggtg gctcacgacg cggccggccg tgaatgcgga accgtgttcg cttttccgag
     8641 gcggaagggc gacagcgcac tggtggcgct cgggtttctg ccgggcacgg aagacctggt
     8701 ggcgtccagt tactatcccg acgtgcgtct gtaccgtttc cgcgtggatg gctcgcaggt
     8761 gacggggaac gggtggcccg cgaatcgcgg ttatgggttg ttgcgccgtt ttaagggacg
     8821 gctctaccac atgggaaccg acgtgttgcc gttgggcgaa aacctgctgg gtgcacgcgc
     8881 gatgcgtgtg ggtcatgaag cgcatcatcg cggatgggcg acagatggtg tgcgtgatta
     8941 tctggcgaca tcgcagggac tctatgtgcg agagccgggg gaaacgcggt ttcgaacgcg
     9001 gcggggcggc atccgcccgc tcacggcttt ggctgtcaac gcgccctacg tttttctgtc
     9061 aatgggcggc acggtgcgct ggctgtatct ggatgaagac gcgacgggcg tctttgcttc
     9121 gtcggacgat gacaacctgc gtgttgccaa cgcttggatt tatccgccgc ttgacatggt
     9181 gccggaagga gcgaatctgc tggtcgctgg cggggaggcc gggctctact gcttccatcc
     9241 gtttctgccg ccgggggcca gtccccgcga ccattgggaa aagagggcgc cgggaccgtg
     9301 tccgcgtgtt ccggggtgtc gggaaacgtt tcccggggtc gaggcggcca tcgtcgccgc
     9361 aggtgccgcg ctcccggagg gtatcgaacc gggtctggtg gcccgccagg gcaagtggct
     9421 tgtcgtggag gatcggaaga atttccgtct tgtccgtttt aaggttttga actaaacgaa
     9481 gaatggtaat gagaggagta aagctatgat gggtaataag acgttgatcg cggccctgtt
     9541 ggccggttgc ggcactgtgg tgtttgcgac ctccttcccg aatgcggacg gttccggcga
     9601 catcgcatca ccaacggctt ggggaggcgt gctccctacg gatcaagctc agttccagac
     9661 gtccgatgtc acgaacacgg catcggcaaa cgtcacgttc gccggtgtcg aggtcggtgc
     9721 gaagaatgtg gtttttgatt tttcccagac gcccgcccgt acgattacac tgagcgacca
     9781 ttttagaccg ctcaactaca cgggcgcgtc ctatactttg cgcggcggga catggaattt
     9841 gagcggaaag aatttccatc tttaccataa ttgggggcag ggaagtgagt gcacggtgac
     9901 actggaaaac gggtgcaacg tcacgaatgt cggttggacc ctgcttaact tttcgacgct
     9961 caaatgccat ttgatcatcc gggatcaggc cagcctgacg gtgaatacct acgattcgaa
    10021 cagtggtcac ggcaatgatg agccttgtct ggaggttctg tcgggtggac gactttccgt
    10081 gccggcgaca ggttcgactt tgcgtatcgg cgatggcacg acattggtct cgggtgcggg
    10141 atcgcgcatt gaaagctacg gcggggcggg tgacttcacg ttgggcaata cctaccacaa
    10201 taccttgatc cggattacgg atggcggcgc ctttgatgcc gcttcacggc ggatgaatct
    10261 gaatttgaat gatgctacga acacgacaat cgtggtggat gatgacggga cgttccaatg
    10321 cggcgaggtc tatcctaacg gtcgtgcctt caatatccaa atcggtgaaa gggggcagat
    10381 gacgacgggg cgctggtata taaaggggaa cgaccattcc gtgagggttt tgaacgggac
    10441 attgaaaggg tcgcgctgga tcggcgaggc ggcttctggg gcttcggtca cgaatttctc
    10501 ggtcgccgtc atcggcagcg atgcccaggt ggatattgcg gggggcgatg ccttgttcat
    10561 gggcctgtcg gattcgtcga ttctgctgga aggggcttcc tggtcctgtc ccgtaaccta
    10621 ctggaaaagc cgtgagtcgt cgaattgcac gttccgtctg acgcagcagg cggcgtttac
    10681 cactacgggg catttctggg gtactgccta tgacggggga tcggcgtcgc agacgaattg
    10741 caatggcaat gtgatcagcg tcgaatccgg ctcgacgttc cagtccgccg ggttcacttt
    10801 cgtcgggaag gggaaccgcc tgatcgttac caatgccacg ttccgctgcc cgacagggtc
    10861 gttcaatttc gggcggtctg acagggggtc ggacgcgaat caacttgttc tgcaaggcac
    10921 gacgccgcgt gtggaaatag caagcgacgc gatgcatttg cgttacggtt caacgttgcg
    10981 cgtggaaatg ccggcggagg ggtatgcggc ggattttgtg ccgatcacga cggcgtggag
    11041 cttgtcgctt gacgactcgt gccatctggt ggcggatgtc gaggcgatca ggcggcacca
    11101 gcgcgtgaac aagacgtatg tcctggccag agccaatggt ggcgtgagca ttccggaatc
    11161 cgtgctggcc gaggccaatg ccgtcgcacc ggacgagtgg cagttcggtg tgagcgagga
    11221 taacaaatct cttaccctgc gtgtgatgct gcttcgggga atggctgtca tgcttaaatg
    11281 aaagttacga tattgccgga gcgggacgcc catgtcccca aaaactttcg tgcccttaga
    11341 taaacgggga taggcctggg ttcatccggc ctcctacaga ctgcctgtca gcgcggagag
    11401 cacgttttct tcggtgatga tccccagcac cgcattgtcc tgagtggcac gtaccagcac
    11461 catcggttgc cggtatgcgc gcatcagggg caagacattg tcggcgcact cgtcggcgcg
    11521 gacaaaatag ggggggtgca tcccgtcgcg tgccagggta cgcttcggat ccggcgcctg
    11581 cgcaaggagg ctcagcacat tcagcacccc aacgcaacgc gagccgtctg cggagaacac
    11641 cggcaaccgc acatgaccgg attcgcgcac cagccggtaa cactcttcca gcggggcgtc
    11701 ctccacggtt ttggtcaccc gcgccaacgg ggtcatcacc tgctccgctt tcatggactg
    11761 caacgttagc acgcggttga tcatcaggcg ctcgaacgcg ctgatgcgcg ctccgttgcg
    11821 tgcgtcactg accacatgct ggatgtactc gcgcgtcatc aaaaaacgct gcccttgggc
    11881 accggctttc tgtggcgtca accacttcgt caaccaaagg accagtgcgg tgagtggcga
    11941 gatggccttc tccacgaaat agaagaggcg gatgatcatt agggtgcggc gcaggggacg
    12001 tgtcgtgaag aacagcttgg gcaggtattc gcagaaaaaa agcagtatgc aggccatggt
    12061 cacggcccat acgctttgga gcgtgcggct gttcggcaga caggtctgcg cgaggctggc
    12121 cgagagggtt gaaaggatga cgttggtcag gttggtgccg accaggacgg cccccatgaa
    12181 acgttgcatg tcggcaaggt atccgttgag caacacggcg gacttcatcc ccgcgcgcac
    12241 cagatgtatc agccgtgcgt ggctgatcga cagcaccccc gcttcggtag cggaaaaaaa
    12301 ggctacggcc gcgaaacagc acaagatcag gaaaatctgg agttcgggga tcatcgcagt
    12361 tccccctcct ccggatggtc atacggcagg tcatcgacct cctgcaggtc ttcctgcagg
    12421 tcctccggtg tctcctccgg gcgttccaac acctccaact gcaccacgtc gatccggcgt
    12481 ttgcgcagtc ggcgcacggt cacgcggcat ccctgcgcct ccacgacctg gcctggctgc
    12541 ggcagacgtt tggcatggaa ggtgatccat ccggcgatgc ggtcggcatc gtctgcctcc
    12601 agttgcaggt cgagacggtg attgagttcg tcgagactga cggtgccatc gaccaaccag
    12661 atgtcgccct gcagattacg gatcgcgggt gtgcgttcct ccccgttgcg ggctaccggt
    12721 acggtgatga gttctaaaat atcgccgcgc gtgatcaggc ccgccgtgcc gccgtactcg
    12781 tccaacacgc aggcgatgtg cttgccgctg cgctgaaaag tcaccagcag gtcgtccagc
    12841 gtcacattct ccggcacgaa cagggcgggg gttgtggctt tacgggcatt gtgcgtggga
    12901 tcgagcagga acatggtggc atccagaaac ccttcgattg cgtccggtgt ccgcacatat
    12961 acgggcagat agcggaactg cgctttgcgg acagtctcga cctgttgtgc gacgggcgtg
    13021 tccaagtcca gtcccaccat gtcaacgcgc ggggtcattt cgtcgctggc catcagttcc
    13081 gacagtctga ggatgccgtc caccatcgac gcctcctcgg catcgatcac gccctgttcc
    13141 tctcccacct cgaccacggt tagcagttcg ttattagaaa gagcgcggcg ttcacggcgc
    13201 aacgcgtgct taaacacgcg cgatgcggcg gtcagcacca gattgaacgg ccgcagcagc
    13261 cagagccaga agagcaaaag tcgactgcat aggggcgcaa gccgctcggc atggagcacg
    13321 gcgatgcgct tgggggtcac ttcgccaaag agcagcagcg cgatagtgac cacggggatg
    13381 gcgatcacct cgccccattg cggagtcagg cgggagaaca tgaggtagcc gatcgtcgca
    13441 atcgcgaagt taaccagagt attgccgacc agcagcgtgg agagaaccag cgacgggttg
    13501 gcaagcagtc gttccaaccg ggcggcggcc tcggcgttgc ggttgcggat ccgctgcaca
    13561 tggatggggg agagggagaa gagcaccgtc tcactacccg aaaaaaaagc cgaaagcgcg
    13621 aagagcgtca gcagcacaat cccgttcagc atgaagaaaa tcggattcat ggtgaggaaa
    13681 cgtcgatcac cgacaggcgg gtccgatgcc cgctgttcaa cgtctcgatc tccatgtcgc
    13741 ggaccatcca gcggtcgtcc atctttttga cgcgctgcac ccacattttg cgcaccggcg
    13801 ttccttgcgg gtcgagttgt tcggcttgca tcaggcagcg caactgccgg tcgacccaga
    13861 cccgcaccgc tgaacacccg gggatcggcg cgggcggcac ggtcaccagg atgtcacagt
    13921 cccggccgtt gcggctttgg ccgcgcggga catcgtcgaa gcgcacatct gtccaccaga
    13981 ggaaatcaag ggtcaggtcc atccaggtca tgtccgtgcc gcggactcgt ccggcatagg
    14041 agggtgagtc caccggctgc tgttcggggc cgcggaacag gctgatctgc gcggggcggc
    14101 ctgcgggtcg cgtgagcacc gcgcgttcga ccagcgaggt gcctgtggca tccagcagca
    14161 gcacctcggc ggaaggcggg cgggcacccc agtcaagcat cagcttgaac ggttcttccg
    14221 cgagtttgat cccgcgcagt ttgcgtaccg taagcgtgcc ttggagcagc agccgatccg
    14281 tggggatcag ttgggtgcag gccgatagaa tctgggccgc ggtcaggttc tcctccaggg
    14341 ccgccgcggc tgtggccgga cgcgattcgt catcagcgcg gcagaccgcg ccgatcagga
    14401 tgacacacaa caaacagacg caaccgggtg tgctcttcaa acggacctct cataacacga
    14461 cggggttcga gaccgcgtgt atgtctcatg tatagcatat ccccggcctc tccgcaacaa
    14521 gcggaatatc attcgcattg cggtacggcc acagaaaacc tatgataccg aatcatgcat
    14581 tcgattctca tggagttcgg gccggtcacc atccgggcgt tcggcctttg tatggcgctc
    14641 ggatttctgt ttggctggcg gacggcggtg ctgctctgca aacggacggg gcaggacgcg
    14701 gatcagctga ccacgctgct gacttggctg atgatctcgg cgttgctggg agcccgcgcc
    14761 gcttatgtgc tcgagcactg gcacgcggag ttcgcggacg cgccgctgat gatcctgcgt
    14821 ttggatcagg gcggtttgat gttctacggc ggctttctcg cggcagcgct ggtcctgttg
    14881 ctctacgcgc gcatccaccg gcaacatgtg ctgtcgatca ccgatctggt gctgacggtc
    14941 gtgccgcttg gccacgcttt cggtcgggtc ggctgttttc tgcacggctg ctgctacggc
    15001 agggtaacca cctcgcgctt cggagtggct tttccgcaag ggtcgcccgc ttggtgggag
    15061 catgtttccg cgaagccgcc gctgatttcg cagcacgcga cggcgagctt gccggtgatc
    15121 cccacgcagt tgatcgaggc cggggccaac gcgttgctct tcgttgtgtt gttcaccctt
    15181 tatccacggt gccgcacgct gcgcggactc ctcaccggtt gttatctgat ggcctacgcc
    15241 acgttgcgtt tcgggatcga aatgctgcgg ggtgacccgc gtctcgctgt ggggccgttc
    15301 tccattagtc aggcgatcag cctcgtactg ttcgccctgg gactggcgct cgccgtctac
    15361 tcgccgcgga tgcgtttgga tgtgcggatc gcacagaaca ctttgccgca catggagcag
    15421 tgatcgtccg cggccccgtc ctgcggggaa cgcgttttga gccagcgggc gcgggcgccg
    15481 gccggatcga gcgccaacgt gaactgcccc tcccagtcga aggccgcgcg cgcgcgggac
    15541 atcgcatcgt cacggttccg tgcgccgggt ctgccccgcg ccacatcgcc ggcatgcgcc
    15601 gcgatgcggt gtgcgatcac cccttgacgg acctcatctg cctccggaag tccgagatgt
    15661 tccgcgggtg tcacataaca gagcagcgag gcgccgtaac aggcggcggc cgtagcgccg
    15721 atgctggagg tgatatggtc atatcccggc gcgatatccg tcaccaccgg tcccagcaca
    15781 tagaacggcg caccgtcaca gaccgcctgc tggcgctcca tattcatttg aatctggtcg
    15841 aatggcacat gcccgggacc ttcgaccatc acctgcacac cccgtgcgcg gcagcgggtc
    15901 acgagtgcgc cgagcacgtc caattcgccg aactgcgccg catcactcgc atccgccaga
    15961 caaccgggcc gcaacccgtc gcccagcgac actgtcacgt catgccgcac cagaatgtcg
    16021 agcacttcat cccagtgcgt gtacagcggg ttctcggcct cgttgcgccg catccactcc
    16081 gccatgatcg cgccgccgcg gctcacgata cccatgatgc gccgcatggc cagatccaca
    16141 tggcgcagta acaggcccgc gtgcagcgtc atgaagtcca caccctgttc ggcctgttcg
    16201 cggatgaccc ccagcagcat ttcgggccgc atgtccggca cgtcaccttc gagacgggag
    16261 agggtctcgt agaccggtac ggtcccgagc ggcgcgggcg aagcctccag catgccctgc
    16321 cggatcagac tcacgtccgg tcccacgctc agatccatca caaagtcggc gccggcatca
    16381 agtgcgatgc gaagtttctc cagttcatcg tgctttcccg agcgggtcgg cgaacgccct
    16441 aggttcgcgt tgactttggt ggagaagaga cgtccgaccc ctacgggacg gactcccgca
    16501 tgcgcgggat tgagagggat caccgctttg ccgcaggcca cggcctcccg cacctgttcg
    16561 ggcgtgacac cctctgtctc ggcaatccgg gccatcgcct cggtgactgt ccccgccttt
    16621 gcggattgga gttgcgtcat gtttctctgt ttccgttcac accgtttcga ccgtcacccg
    16681 atcacacggc agacgcgtgg gggtgcccag caccgttgcc acctcgccga cctgcgcacc
    16741 ggtgcggatc gattcataca catacgcctt gccctcggtc accgcctcga ccaacggacg
    16801 cccgagcgcc agcgaagccg caatggccgc gctgagcgag cacccggttc cgtgcgtcga
    16861 cgctggcgac accagtcgcg gtgttgtcac acgatgcaat gccgcaccgt catacagcac
    16921 atcttcggcg cagggcgagt cggcatgtcc tcctttgacc aacaccgcac agccgaagcg
    16981 atccgccagt tcgcgggccg cctcctgcat cgcctccaac gtcagcaccg tccgatccga
    17041 acccagcagt acttcggtct cggccaaatt gggcgtcagc agggtggcca aaggcaacag
    17101 ccgcgacgtc aacatctcca acgcatcctc cgcgagcaga cgcgcaccgc tcgtcgcgac
    17161 catcacggga tcgatcacct tcgcgatccg gttgtggcac accagacagt cggccacggc
    17221 ctcgatcacc tcggggcagg cgagcatgcc ggttttgagc gccgtgatgc cgtacacgtc
    17281 gagcacggta tccaactgct ctgccacaaa tgcggcatcg gcggtcagta cggcacgcac
    17341 gccgaaggga ttctgtgcgg tcagtgccgc gattaccgtg cagccgtgca cgccgaaaac
    17401 atggaaggcg cgcagatccg cctgaatacc cgcattgccg ccgctatcgc tacccgcgat
    17461 cgttaaggcc acgggcaacc gcaccggctc cagacacgcg cattcgtcgt ttcgctcgtc
    17521 gctcactctt ccaccaccac cggcaggccg ggctcgacca aattcaggaa acggaccgca
    17581 ttccagttgg ccaaccggaa gcagcctttg gattcggccc ggccgatctg ctcggggaac
    17641 ggcgtgccgt gaatgccgta gccctgcagc gagaggccca cccaggcgag gcctaccggg
    17701 ttgttgggcc ccggcgggat caccaacttc gatcgcttcg gacctttcgg atcaaacagg
    17761 tgcgggtcat acagatagtt cggattggga gcgatatttt tcaccgtcag ctcaccgtca
    17821 gggcggtgcc gcttctcccg cgcgatcgag caggggaaga gtgcgatcag tttgcccgcc
    17881 gcgtcgaaca ccgtgacctc catgcggcta agcgcgatgc gcacggaacc ggctttgacg
    17941 gacgggacgc gcgtagtgca atcgggcagc acgatctccg tgccgacggg cggattgggc
    18001 cacgcgacct ccgggttcag acgcgccagt gcccgctgag aggtgtggcc ctgctccgcc
    18061 accttctcct gaatcgtctc gtacgtcatg ctgggaagtc gcgcccgttc ctcccaggat
    18121 tccggaaccg gcccgatcgc ggcccggtcc gcttctgtca ccgtgtgccg ggtcaggaca
    18181 tccgcatccc cgcccagagc ctccagcact gcggcgtccg gcacaccggt ggccggcagt
    18241 ccgttcaagg tctgccacgt catcagggcg atctgcgttc gtttgcccca cataccgtcc
    18301 acgcagttgc acgacaagtt gcgacggtcc agcaacactt gcagactcag cacatcccgg
    18361 ttaaagccgg aacggtatcg gacgaagcgc tggcttgtgg ccgaagtcgg ctcggcgccc
    18421 gtcacggccg aagccagact gatgcccgcc agccacatgc caacctgttt tgcgaacccc
    18481 accgtgccga atcctttccg tttagcaatg gcccgcatta taccacaagc ggtagttcgg
    18541 tcgatatcat tcctgcctct ctgtccggcg cgacgatctg ccatacaatc ccaatgcggg
    18601 cggagcccgt aacccgattt agtcacacac aaaggttttt tcatgacatc ccatttccct
    18661 tcgtcttctt ctattggcct gatcagccga tacggcacgc atgtccagac gatgatcggt
    18721 tgcatgtgga aggtgcgtgt cagccaaaca gggcgcgtgc attacgggtc gtgatttccg
    18781 ccacccgttc gggtcgggtc ttgcgcacag ccgccagaca ggcggccaca tgggtcacat
    18841 acgcgggctc gttgcgttgc ccgcgcatcg gcaccggtgc cagataaggc gagtccgttt
    18901 cgatcaggag ccggtcatcc ggcacgaagg ccgcgctggc gcgtacgctt tcggcctgcc
    18961 ggaaggtgac gatcccgctg aagctgaggg ccatctggcg gtcgagcaac tgcgcggcaa
    19021 accgtgtgtc gccggtgaaa cagtggatca ccccgcgcag accctggccg tgccaaggcg
    19081 tctcgtccag cacgcgcagg gtcgcgtcgt cggcttcgcg cgtgtggacg atgaccggtc
    19141 gtttccagac gtcggccagt cgcaattgcg ccgcgaagag tgcgcactgg gcctctgcgg
    19201 tttcgggatg aaagtggaag tccagtcctg tttcgcccac tgccgcgaac ggatacgttt
    19261 cggcgagttg ccgcagtgtc tccacaaaga cctccggttc ggcttcggtg gcctggtcgc
    19321 ggtcgaaccc cagtgccaga cggatcgcat ccggaaacgc ttgggctgcg gcaagggcgc
    19381 cggcgttcag tttcgcgttg ccgcccaccg ccaccacacg gctgaccccg gcttcgcgcg
    19441 cccggctgag cacggctacg gtctctgcgg cggtcagggt gaaatgcgcg tgcgtgtcgt
    19501 aataggtttc agtaatcacg atggctccgg attgcggggc gtttccctgc gcggcgtcac
    19561 gcgtcacacg ggaaaaacga aacgccgctc gaacgaaaag gcgttttcca gatggcggat
    19621 cagcggctcg ccctcttcag gctgccccag aacctcaatc acatcgaaac ggtaatacag
    19681 atccggaaaa cgcgccttcc ggagataggc cacggccgcg cggttcagcg cgtgacgttt
    19741 cgccggattc accgcccgcg ccggacgggc gaacgtttcg cggtggcgcg tcttgacctc
    19801 cacgaacacg agggtatcgc cttggcggac gacgagatcc agttcgtcgc ggcggttggg
    19861 ccggacattg cggcccagca ccgtataccc gcgcgcggaa aggtacgcgg cggcttgctc
    19921 ctcgccccat gcgccgacac gggccgaacg cggaagttct ttccgttccg tctctttgcg
    19981 acggaggttg agggtgcgct gcagcagttt ggagagcgcg gacaccgcat caccccgata
    20041 tagcctgacg gccggtatca aaggcgcgga ggttcagttc gatcagcgca cccgggatcc
    20101 gttcgcgcat cgcctgtttc cagatttcgg gttcgaacgg caacaaggcc gagagcgcac
    20161 ccgcgatcac catgttcatc gtgcgcatgt tgcccatctc cttggcgatc gtcagcgcgt
    20221 caacatacag cgcggacgga tattcggcgc gcagacggtc atccagatcc gtcaccgcaa
    20281 cctgctggcc gctcgaaacc gtgatcggcg tcatgtccag actgttgatc agcactttgc
    20341 cgtccggcgc gagcaggtgc cgccagcgca acgcctccag ccgctcgaac gagaccagca
    20401 ggttactcgc gccgtccggt atgatcggcg agaaaacctt cttaccgaaa cgcacctggc
    20461 tgatcacgct gccgccgcgc tgcgccatgc cgtggatttc gctctttttg acgtccagac
    20521 ccgcgaggac cgcggttttg gcgaggatgt cgctggtgag caggatgccc tgaccgccca
    20581 cgccaactaa ggaaacgttt gtgacgttct cactcatgct cttctccttc agggggggta
    20641 caagagcttc tttcgattcg cccgcgcgcg acccccacgt aatcaacttg aacctttttg
    20701 aacagatgaa catcctgatt gcgctcaata ccaacataac tccgcccttc caacgcagca
    20761 gcaa
//
LOCUS       DAOSRB010000025        19832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2908_length_19832_cov_7.700258, whole genome shotgun sequence.
ACCESSION   DAOSRB010000025 DAOSRB010000000
VERSION     DAOSRB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19832
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_19832_cov_7.700258"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..396)
                     /locus_tag="PLZ74_02570"
     tRNA            complement(309..396)
                     /locus_tag="PLZ74_02570"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Ser,seq:tga)
     gene            complement(574..2238)
                     /gene="ilvD"
                     /locus_tag="PLZ74_02575"
     CDS             complement(574..2238)
                     /gene="ilvD"
                     /locus_tag="PLZ74_02575"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOR97237.1"
                     /translation="MTSDQVKTGFQRAPHRSLLMATGVPRQDFKKPFIAVCNAYTDII
                     PGHCHLNQVGEWVKECIREAGGVPFEFNTIGICDGIAMGHTGMKYSLPSRELIADGVE
                     SMVRGHCFDAMICLPNCDKITPGMILGALRCNIPTLFASGGPMRAGRLDGKAIDLISV
                     FEGVAQFNNGKIDEKQLEAIESSACPGCGSCSGMFTANSMNCLCEAIGLALPGNGTIL
                     ADDPARRELWKRAAQRVVEMALAGGPRPRELITLEAVDNAFALDMAMGGSSNTVLHTL
                     AIAREAGLDYDLDRINAISQRCPNVCKVAPSCAYHMEDVDAAGGIPAILKRIADIPDL
                     LDTTCPTVSGLTIGEIIAAAEIKDAEVIRTLDNAYSKDGGLTILRGNLAEQGAVVKKA
                     GVAASMLTFEGRAICFDSQEEACEGILDGKVKAGHVVVIRYEGPRGGPGMQEMLAPTS
                     YIMGAGLGESVALITDGRFSGGTRGACIGHISPEAAEGGLIGLLRDGDPIRIDIPGRR
                     LEALLDEAEIARRRAAAPAWKPRDLGGWLRRYALSVGNASSGASLL"
     gene            complement(2288..2363)
                     /locus_tag="PLZ74_02580"
     tRNA            complement(2288..2363)
                     /locus_tag="PLZ74_02580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2328..2330),aa:Thr,seq:tgt)
     gene            complement(2492..4045)
                     /locus_tag="PLZ74_02585"
     CDS             complement(2492..4045)
                     /locus_tag="PLZ74_02585"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOR97238.1"
                     /translation="MSEQTQTEMRRIYMFDTTLRDGEQSPGCTMSLRDKLLVAEHLEA
                     LRVDIIEGGFAIASPGDFEAVKSIAGVVKNAAVASLCRALPKDIDRTWEAIRSARHPR
                     IHTFIATSPIHMEFKLNMTPDQVYDQAVAMVRYARNLCGDVEFSLEDCSRSERPFIYR
                     IIEGVIAAGAGTINIPDTVGYAVPEQFGQLIADIRVNVPNVDKARLSVHCHNDLGLAV
                     ANSLAAIRAGADQVEGTINGIGERAGNTALEEVVMALHTRREFFQAQTGIDTTKIYAT
                     SRCVATVTGSRVQPNKAIVGENAFAHEAGIHQHGVMANPLTYEIMTPESIGLPRNKMV
                     LGKHSGRHAFESRLRELGFPLDAERMQELFVRFKDLADRKKNISDADLEALAQDVQSM
                     VTEHVKLDRFNVTASNAITPVSTIRLLRDGKLVEQVAASDGPINASFKAINQILGLKV
                     TLESFQLTAITGGTDAQCEATVRVRDGDHIYHGRGVSTDVIEGSILAYLQALNSLLDE
                     AAETSKRPA"
     gene            complement(4370..4681)
                     /locus_tag="PLZ74_02590"
     CDS             complement(4370..4681)
                     /locus_tag="PLZ74_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97239.1"
                     /translation="MKETRHSKTQSAHARAVLFLCERLTNRGHTVISANTDEKADPQI
                     FAQSETGELAFYFVRTGTSTPPEDDLARFRALAAHHRVAAYFAPVTFSPAPHCAEVRP
                     L"
     gene            complement(4805..5368)
                     /locus_tag="PLZ74_02595"
     CDS             complement(4805..5368)
                     /locus_tag="PLZ74_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOR97240.1"
                     /translation="MNDTIRLILVLTVICSISSALLAAVYRQTQAPIEATLRQRTLRT
                     AATVLPCGSDTPTPVELDGTTFYVARQGGAFAGAALEGHSANGYGGDMVLMVGLSADG
                     KLVNFKKLVGSETPGLGTKIDTDLFRKPLLGRSLKANWTVRKDGGEVDAVTAATISSR
                     AALECIRDAIARFEKALPSLTAAPSDR"
     gene            complement(5365..6429)
                     /locus_tag="PLZ74_02600"
     CDS             complement(5365..6429)
                     /locus_tag="PLZ74_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOR97241.1"
                     /translation="MKPEASPDLFIISPSPHIHAGSSVRRIMLDVLIALLPALAASLW
                     FFRWHALRLTVTCVATCLLTEWLCRKAMRRASTLSDLSAAVTGLLLAFNLPPALPTWM
                     AVAGSVFAITIAKQVFGGLGYNPFNPALAARAFLLISFTGAMTSWSGSPWIRKVTYAA
                     SEATTKATSALAADALTSATPLGLAKEALKSGQGMPLFSDPTLLRDLFLGNINGCIGE
                     TSALALLIGAAYLLFRRVITWHIPGAFLASVLVYAGILHAVSPAASLPPHVHLLSGGL
                     LLGAFFMATDMVTSPLTRRGMLIFGAGCGLLTMLIRTVKTGAYPEGVSFAILIMNAFT
                     PLINRATRNRVFGTQPHPKP"
     gene            complement(6461..7804)
                     /gene="rsxC"
                     /locus_tag="PLZ74_02605"
     CDS             complement(6461..7804)
                     /gene="rsxC"
                     /locus_tag="PLZ74_02605"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOR97242.1"
                     /translation="MPTIPSSFTFHGGIHPDYHKELAAGSPIRELPLPARLVVPLSQH
                     LGASAKPVVQTGSTVIGGQLLAEADGLISAPVHAPAAGKVVALEPTLTATGRACDAIV
                     IETASEQTWESLPPLPEWRTADPKSLIERIGVAGVVGMGGAGFPTRVKLSPPRDKPID
                     TVILNGAECEPYLTADHRMMLEHAADIRLGAEIICHILGAKTLRVAIEDNKPDAIEVL
                     EKIFQDMDGDVAVTVLRTRYPQGSEKQQIFSVTGREVPRGGLPMDVGCVVENVSTAFA
                     IAEAVARGRPLVRRVITVTGDAIAQPANLLVPCGALYSDLVAACGGITGKVSKVISGG
                     PMMGFTVSNLAVPVGKTASGLLLLSPKNTTCFTSQACIACGRCVDACPMRLSPAELSQ
                     CIEAEDIEAAEPLAVMDCIECGSCAFVCPAHRPLVHHMRRAKAHIMARRAAAKTS"
     gene            complement(7836..8633)
                     /locus_tag="PLZ74_02610"
     CDS             complement(7836..8633)
                     /locus_tag="PLZ74_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOR97243.1"
                     /translation="MDLITTSTLCVGGIGLASAAALAAAEKYFTVKQDPRIDQVTELL
                     PGANCGGCGYAGCADYARAIVDGTAECNLCAPGGPDCAQAIAALLGRTVAVLEKRVAI
                     VLCCGDNTEATRRATYNGIADCASAQTIAGGDKACTYGCLGYGSCARICPVNAIHMLN
                     GIAKIDKKRCIACGKCVAACPRRLIKLVPAAAEIHVLCSSKDKGPAVKKVCGTGCLGC
                     SICAKLSDGAISMDGFLAMVDYSQPLTHEEVVAKCPGHCIRKDPPNS"
     gene            complement(8653..9231)
                     /gene="rsxA"
                     /locus_tag="PLZ74_02615"
     CDS             complement(8653..9231)
                     /gene="rsxA"
                     /locus_tag="PLZ74_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOR97244.1"
                     /translation="MEYLLLLLSAILVNNFVLSRFLGICPFLGVSKRISTALGMSGAV
                     IFVITIASAVTWCLQTYVLAPLGLDKFLQTLAFILVIAALVQLIDIVMKRFTPSLHAA
                     LGIFLPLITTNCAVLGVAVLNMKLGYGLFKSVLFGFAAATGFAFALLIFTGLREKIDR
                     ADPPRCFRGVAISLVTAGLLSLAFMGFNGLVK"
     gene            complement(9659..10249)
                     /locus_tag="PLZ74_02620"
     CDS             complement(9659..10249)
                     /locus_tag="PLZ74_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase ThyX"
                     /protein_id="HOR97245.1"
                     /translation="MSQPPILKYFKTKSVWRDVADAARTTIRMEEGDREPSNAWKHRI
                     LLSEHSPIRQMLFKWKWVNLKYWVSVHFVRHKIGIEHFVSTQRSDRTGVNRDTMPQAS
                     LVDHECFANAQALIFISRKRLCKQASPETTEAWRLVLDAMREHEPELYRVCVPECVYR
                     GFCPEFKSCGYAQTEAFRQALDAYRSGNDANAARKD"
     gene            complement(10288..11025)
                     /gene="fabG"
                     /locus_tag="PLZ74_02625"
     CDS             complement(10288..11025)
                     /gene="fabG"
                     /locus_tag="PLZ74_02625"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOR97246.1"
                     /translation="MKTALITGAAKGIGRAIALGLAHDGFRIALHYHTSVEAAEETVA
                     AIRKAGGKAEPFAADLTVPAEVDELVKRVIGTCGGIHTLVSNAGILRSQLLAFTGLDT
                     WREVHAANLDAAFLLTKALTRPMARQQAGRIIYIASAAGLMGDLMRAAYSSSKAALFG
                     LAKTAARELAASGVTVNVVAPGIITTDMTAEIPETRRVKQLAAIPLGRFGTPDEVAGV
                     VRFLASEEAAWITGQILRVDGGLCPSP"
     gene            complement(11022..11480)
                     /locus_tag="PLZ74_02630"
     CDS             complement(11022..11480)
                     /locus_tag="PLZ74_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOR97247.1"
                     /translation="MTQILIPQLDANVVEVTLSRWRKQVGDTVEAGECVAELATDKAA
                     YELEAPASGTLLAVYATAKSVVPTGFIAGLIGAPGEEDPSIPEKNAALTAAYRGTASA
                     GSGTDRGTRIRATPRARRLAREHHIDLEKVRAETGADLIDETVLAPYLPA"
     gene            complement(11518..17277)
                     /locus_tag="PLZ74_02635"
     CDS             complement(11518..17277)
                     /locus_tag="PLZ74_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97248.1"
                     /translation="MNWTNVLIWAGLTWGCCAATAGLAAEEAWSTRLDLTERSEWGAL
                     NLTPERGALRLRLSHPDHTCQAEFTLSEGDTRFRLLPRGFFDDATERRVTTYPCPSLA
                     GPLHWITLKRHADVWILTVDDRPVARLPELWQGAVNVEHPPALQPPEAEADEYTQRLG
                     SFTFEDNFLVPAGSAFPPTWDIQSGIWRLHSVTGSISGSSGGYQLARQPKPEKSPNFY
                     TLEGGGTNAIVLAGEPFYSRYVFRAAVQHNTGTNGLVFLAGEHGGYFAFTARTDPVSD
                     RLILDLWQQPAAPNAPRLYLAGVQTELPAGQWLLLEVQTFDDRILCRADNIEVIRHKI
                     GLPPGGRFGLFANMPAGETTRFDDVTAASHQDRLFDSPEDVTFATRRQSPDVRSLQRH
                     DTAWLYFPPSATTTQMWAYGSSDDGPLRQEIRVVATTDSFLCGLTAGGSGDGAAPYRF
                     TCEQDGGGRTFRLEHVTTNGTVLLDSFQMPVSSNRVILALDALRPHELRAYADDLLVC
                     FHRPEDQPGGELGVLAVTPGDLFFTAPTVTSRETGLSERFEKNPLYVNDPFMRHWASP
                     EGQWITFKNGMTWFKGDITGAVRVRLPVVEEMELHLGVPEGLSNGLCRVSVKKGVIRV
                     FTPESGTNTAFEVSADQVPEVEFNNTKLRLFTLGLEGSVLWLGGDTLLLAQTHLHAPL
                     HGRRMRISGMTLDNLSRTLVKRDNVFDTLFTESLYNWTINGGRWEVINRFYCEPTWSH
                     MNGESADSLAALWSKYVFRGDFSIEFYAGMRMGWYARPGDLNLTMMSRSLSTGDGYTA
                     IATGWDPDHSQLYSRLIRNGSLLDLSTKYLVPRARTGLARQGYQPLVAGGRDIHGAWY
                     GMQLRRVGKTLHYIYDNEDVFRIEDPDPLQEGALGIWTYRNSMMVARIKIAAESIRPR
                     PFAFRALPPAAPPPTEPAAAPADTGIRINGRVAQPLTPDFWQAYDTVSHPNVRFRHLQ
                     TARPEMYVTSLLGAGSFLVRCMLPPAQPDKLLGWRFEIARHPQARVNFEFATVKEDGK
                     GGWTPIQHWTYVLCGSNESRGPRKICGALESLPASDPAGTNRVWTPVEIWVPSEIIRA
                     NQAVQIEGFGNLQPSDVQQGLEGNPPHAWYAIRHFREIHRGIPTVSGPPEKRAEIASL
                     TQLINGLSPGELQMVELPAALDSRRPVIEWAVPERAKFGLLAQSDPAVLGSIVVTPTH
                     PWPSPMLPPKQVQVDAQPAPFYTEGNTIRILVPLNILQPGRMTLALELYDGRFFRQVV
                     PMRKEDSVNSPPVLLSLTMPEGAVETFEARPFDTTPHKMAARVAVEVTDPRRGGVLAF
                     SNAGVYGRRLTGQLFPRFDIAATPLLQFRYKGDPMAIVSLAFGEFAFTFSEAFNTSVR
                     FTDNARAEMDDTWRVWTGIPSDSVGRFPLAKRISLPPAPIRLASRQTRDQTGLHSTLR
                     IDDLTCGPAAGPNRPFAFKADYADPDGVAEIAYAILPGPAVWDNRSADEQQKTAWLPG
                     KNAAVIEPDLTALADGIHHLVVRARDGQGLWSQPTDVPFLFDRKPPEIHHALTDVDHY
                     NGSCLTITLTDPVAPPVLNTLRLTHLGTPINLKQDNGFCTVGQGKIAFELDWIWLLRE
                     QLAAVKHGATLPLSLGGITDAAGNALPPVKIEIPVNLEKDTRPPTILPTAFPTNMLWC
                     EPALTTLDPFFTESQNVQATTGVTPDGRALEITANGERASYIRHTFGPTWDPDIYPWL
                     AISFRTVGEPREGRPFTLAFNTGARRPRGIKEAHTLNLKDTNHLAYVTGALNLQTGVW
                     NDVLIDVRSFLLQETDEHKNTPDITYLSLFFRHKAKGSAVQIRSLSILSPWHSALQIP
                     IRAYDLSTVAGLQWKGGRSQATGIRPANLQLPPDDPLWFRFRLQDGRGNLTDTWMIPV
                     PPGSQNSNPALSGLEPVVF"
     gene            complement(17298..18863)
                     /locus_tag="PLZ74_02640"
     CDS             complement(17298..18863)
                     /locus_tag="PLZ74_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOR97249.1"
                     /translation="MIGVMSYTPRLTKPVPAEYLRDESRKTGVAEAIAFPRDRSELTA
                     LLRAADTAGLPLTPQGARTGIAAGAVPDGGLVINLSRMDRILACCRETPDRTLLRVEP
                     GLPLVLLQRHLSDRRLFPDPLFFPPDPTEPSATLGGMAACNASGARSFAYGATRRHIE
                     AVTAVLADGDTLTLRRGRERAAGRRFAVTTESGRRLEGDLPLLPVPEVKNAAGYAARP
                     DMDLIDLLLGSEGTLAILAELELRLSPAPAGILGIVAFFNEEPQALTLVEQIRQQAAA
                     APSPLLTAIEYFDAEALLLAHEGAAQTGVPLPSLPSRGRNAVYLEWALNDGPDAALDR
                     TARLVAAAGGNAVDTWVAHETVALGRLKTFRHAVPEQINALIAERARAHPGLTKLGTD
                     FSVPDAKLRPLLRRYREDLTAANLQHVIFGHIGNNHLHVNILPRNMDEYVMGKRLYHS
                     WAAQVVAWGGSISAEHGVGRLKRELLREMVGAEGLKAMRRIKRLFDPAGRLNPGCLFD
                     PNVPSAFSLPPPV"
     gene            complement(19164..>19832)
                     /locus_tag="PLZ74_02645"
     CDS             complement(19164..>19832)
                     /locus_tag="PLZ74_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97250.1"
                     /translation="LLINGAQGDTNHLRPKTAPGEIVPRRYEMSRHMARTIAGAALAT
                     WGMCAPAPSGKVHAAMPKVKVLTNKGKPEELPLAEKYVRLHKSGRANEIPGIGMERTA
                     NTAGAYRILELKDAPDEVDLRVSVVTVGTTLAFGGFPGEPFTWMGTELKAKSPFAMTI
                     PACAVNGSRGYFPVKSAYGPGGYENATSRFKAGTAERLVDGALLKLAELYKAAAPCET
                     KSAK"
BASE COUNT         3525 a         5741 c         6828 g         3738 t
ORIGIN      
        1 gcaggtcgcc cgtcgcgggg cgacataagg tctgacggga gaagcgggcg tccctgcccg
       61 ctggtaggga cggttccccg aaccgtccgc ggacgcttgc ccctcgtgcg ccccgcgagc
      121 ccgggtgccg tacacgttca cagcgcggcg gttcgcgttc acgcacacgg cgacgatatc
      181 ccttggacgc caaaccggtc ggcttcgcct ccctgccgca cgctgcggtg cggcccctgc
      241 ctgctgcgca ggcggtcgaa ccgggggttc tcatccctcc ctgttcagac gaaggctgga
      301 aagtagaatg gcggaggggg agggattcga acccccggta ccttgcggca cgccggtttt
      361 caagaccggt tctttaaacc actcagacac ccctccggaa cagatgacgt gcagtatagg
      421 tgaaagtccg ggccacttca agccgatgcg cggaaagctt tgatggccaa gcgctgaacc
      481 ctttggcgaa acgggatagc gggcaagacc ccgactccgt cacgcacgca tcggggagac
      541 gctcttgccg tcggcatgct atgcaacccc ggattacagc agtgaggcac cggaactggc
      601 gttgccgacc gacagcgcgt aacgccgcag ccatccgccg aggtcacgcg gtttccaggc
      661 cggtgcggcg gcgcggcggc gcgcgatttc cgcttcatcc agcaacgcct ccagacgccg
      721 tccgggaatg tcgatgcgga tcgggtcgcc atcgcgcaac agaccgatca gaccgccttc
      781 ggctgcttcg ggggagatgt gcccgatgca ggcgccgcgc gtaccgccgg agaagcggcc
      841 gtccgtgatc agcgccacgg actcgccgag gcccgcaccc atgatgtagg aggtcggcgc
      901 gagcatctcc tgcatgccgg ggccgccgcg cggaccctcg tagcggatga ccaccacatg
      961 gccggccttg accttgccgt cgaggatgcc ttcgcacgcc tcttcctgcg agtcgaagca
     1021 gatggcgcgg ccttcaaagg tgagcattga ggcagcgaca ccggcctttt tgaccactgc
     1081 accctgctcg gcgagattgc cgcgcaggat cgtgagaccg ccgtccttcg agtaggcgtt
     1141 atcgagcgtg cggatgacct ctgcgtcttt gatctcggcg gcggcgatga tctcgccgat
     1201 ggtcaggccg ctgacggtcg ggcaggtggt gtcaagcaga tccggaatgt cagcgatacg
     1261 cttgagaatc gccgggatgc cgcccgcggc gtcgacatcc tccatgtggt aggcacagga
     1321 aggcgcgact ttacagacgt tggggcaacg ctgggagatg gcgttgatgc ggtcgaggtc
     1381 atagtcgagc cccgcctcgc gcgcaatggc gagtgtgtgc agcaccgtgt tggaactgcc
     1441 gcccatcgcc atgtccagcg cgaaggcgtt gtcaacggct tcgagggtga tcaactcgcg
     1501 tggacgcggc ccccccgcga gggccatctc gacgacgcgt tgcgccgccc gtttccagag
     1561 ttcgcgtcgt gcggggtcgt ctgccagaat cgtgccgttt ccgggaagcg ccagtccgat
     1621 ggcttcacaa aggcagttca tggagttggc ggtgaacatg ccggaacagg atccgcagcc
     1681 gggacaggcc gacgattcaa tggcttcaag ctgcttttcg tcgatcttgc cgttgttgaa
     1741 ctgggcgacg ccctcgaaca ccgagatcag atcgatggct ttgccgtcaa gacggcccgc
     1801 gcgcatgggg ccgccgctgg cgaaaagggt ggggatgttg cagcggaggg caccgaggat
     1861 catgccgggg gtgatcttgt cacagttggg caaacagatc atggcgtcga agcaatggcc
     1921 ccggaccatg gactcgacgc cgtcggcaat cagttcgcgg ctgggcaggg aatacttcat
     1981 gcctgtgtgc cccatggcga taccgtcgca gatgccgatg gtgttaaact cgaacgggac
     2041 gccacccgct tcgcggatac attctttgac ccattcgccc acctgattca gatggcagtg
     2101 cccgggaatg atgtccgtat aggcgttgca cacagcgatg aaaggctttt tgaaatcctg
     2161 acgcggcaca cccgtggcca tgaggaggct acggtggggg gcacgctgaa agccggtttt
     2221 gacttggtcg ctggtcatgg tgttcttcct taattattat tcggcttcgc tatggtcgtt
     2281 ttttgaatgg agccagcaga cgggttcgaa ccgacgacct catgattaca aatcaagtgc
     2341 tctaccaact gagctatgct ggctaaaagg gtggcctgcg caacggacgg gaaagaacct
     2401 gccgcgcgcg ttgtacgcct tgaaacgtaa ccggtatgat cttacgataa caccggttca
     2461 cgtttcaagt cgaaaggggc gcgcgcgccg gttacgcggg ccgtttggac gtttccgcag
     2521 cctcatccag caggctgttc agggcttgca gatacgccag gatgctcccc tcgatgacgt
     2581 cggtggaaac gccgcgtccg tgatagatat ggtcgccgtc acgaacccgg acagtggcct
     2641 cgcactgcgc gtctgtgccg ccggtgatgg cggtgagctg gaagctctcc agcgtgactt
     2701 tgagccccag aatctggttg attgccttga aagaggcgtt gatcggtccg tcacttgcgg
     2761 cgacctgctc gaccagtttc ccgtcacgca gcaaccggat cgtgctgacc ggcgtgatcg
     2821 cgttgctggc cgtgacattg aaccggtcga gtttgacgtg ctcggtgacc atactctgga
     2881 catcctgcgc caaggcctcg aggtcggcgt cgctgatgtt ttttttccgg tccgccagat
     2941 ccttgaaccg tacgaacagt tcctgcatac gctccgcatc gagcgggaag cccagttctc
     3001 gcaggcggct ctcaaaggcg tggcgccccg agtgtttgcc gagcaccatt ttgttgcgcg
     3061 gcagaccgat cgactcgggg gtcatgatct cataggtcag cgggttggcc atcaccccat
     3121 gctggtggat gcctgcctcg tgcgcgaagg cgttctcgcc cacgatcgcc ttgttcggct
     3181 gcacgcggct gccggtgacg gtcgcgacgc aacggctggt cgcatagatt ttcgtggtgt
     3241 cgatcccggt ctgcgcctga aaaaactcgc gccgcgtgtg caacgccatc accacctctt
     3301 ccagagcggt gttgcccgcc cgctcgccaa taccgttgat cgtgccctcc acttgatcgg
     3361 cgccggcccg aatcgcggcc agcgagttgg cgaccgccag gcccagatcg ttatggcagt
     3421 gcacagacag ccgtgccttg tcgacgttgg gcacgttcac gcggatgtcc gcgatcagtt
     3481 ggccgaactg ctccggcacc gcgtacccca cggtgtcggg gatattgatt gtccctgcgc
     3541 cggcggcgat taccccttcg atgatccggt agatgaaggg ccgctcgctg cgggaacagt
     3601 cttccagcga aaactccaca tcgccgcaca ggttccgcgc gtaccggacc atggcgaccg
     3661 cttgatcata gacctggtcc ggcgtcatgt tcagcttgaa ttccatgtgg atcggcgagg
     3721 tcgcgatgaa ggtgtggatc cgcgggtgcc gggcgctgcg gatcgcctcc caggtccggt
     3781 cgatgtcctt gggaagcgcg cggcacaggc tggcgaccgc ggcgttcttc accacgcccg
     3841 caatggattt gacggcctca aaatcaccgg gcgaggcgat cgcgaagccg ccttcgatga
     3901 tatccactcg cagcgcttcg aggtgttccg ccaccagtag tttgtcgcgc aggctcatgg
     3961 tgcaacccgg ggattgttcg ccgtcgcgca gggtggtgtc gaacatgtag atgcgtcgca
     4021 tctctgtttg cgtttgctcg ctcatggttt ttcctcgttg gtttatgctg tgcggtgtgc
     4081 cgctggcccg tacgttgtga caaaaacaaa aactcccgcg gaatcgtttg agattacctg
     4141 cgggagtcct taccgttcac ctgtatgccg atccgattac acggtaaccc tcctgcaggc
     4201 ccgcaagagc cccagtccgt taagtcggaa caagcaaaca gtggatttgt cattcatcag
     4261 cgcacactat gtcataagac cgggggggcg tgcaaggcga aaaaggggcg ccgcgcattt
     4321 tttttggggg atcccgtgtg ccgtccgctg cggagcggtt tacgggacgt cacagcgggc
     4381 gcacttccgc acagtgcggc gcgggggaga acgtgaccgg cgcgaaatag gcggcgaccc
     4441 ggtggtgcgc cgccagcgcg cggaaccgcg cgagatcatc ctccgggggc gtcgacgtcc
     4501 ccgtgcgcac aaaatagaag gccagttcac cggtctccga ttgcgcgaag atctgcgggt
     4561 ccgccttctc gtccgtattg gcggaaatca cggtatggcc acggttcgtc aaccgttcgc
     4621 agaggaagag gacggcgcgc gcatgagcgc tttgcgtttt gctgtgccgc gtttccttca
     4681 tgttgttctc tcctgccgct ccggcggatt tgtccgggca gaattcgagc aaagcctcgt
     4741 aaacattggc ttccgtcgcg atccgcgccg cacctgtttg gggagggacg agcgtctgcc
     4801 cgtctcaccg gtcggacggc gcggcggtta gactcggcaa ggccttttcg aagcgggcga
     4861 tcgcgtcgcg gatacattcg agcgccgcac gggaggagat cgtcgcggca gtgacggcat
     4921 cgacctcgcc gccgtctttg cggacggtcc agttcgcttt gagggaccgg cccagcagtg
     4981 gtttccggaa aaggtccgtg tcgattttgg tgccgagccc cggagtctcg gaaccgacaa
     5041 gtttcttaaa attcaccagt ttgccgtccg cactcagacc gaccatcagc accatgtcgc
     5101 cgccgtaacc attggccgag tgcccttcca gcgcggcacc cgcgaaggcc ccgccctgac
     5161 gggccacgta aaaagtggtg ccgtccaact ccaccggggt aggcgtgtcg cttccgcagg
     5221 gcaggacggt ggccgcggtg cggagcgtgc gttgccgaag agtggcttcg atgggggctt
     5281 gggtttgacg gtaaaccgcg gccagcagag ccgagctgat tgagcagatg acggtcaaca
     5341 ccagaatgag tcggatcgtg tcgttcatgg tttcggatgg ggttgggtgc cgaacacgcg
     5401 gttacgcgtg gcgcggttga tgagcggcgt gaaggcgttc ataatgagga tggcgaagct
     5461 caccccttcg ggataggcgc cggttttcac ggtgcggatc agcatggtga ggaggccgca
     5521 gcctgcgccg aagatcagca tgccgcgccg ggtgagcggc gaggtgacca tatcggtggc
     5581 catgaaaaac gcgcccagca gcaggccgcc ggagagcagg tgcacgtgcg gcgggagcga
     5641 ggcggcgggc gagaccgcat gcaggatgcc ggcgtagacc aggaccgaag ccagaaaggc
     5701 accggggata tgccaggtga tgacccggcg gaacagcagg taggccgcgc cgatcagcag
     5761 ggccaaggcc gaagtttcgc cgatgcagcc attgatgttg cccagaaaga gatcccggag
     5821 cagtgtcggg tcgctgaaca gcggcatgcc ctggccggat ttgagcgcct ccttcgctaa
     5881 ccccagcggc gtggcggagg tcagggcgtc ggctgccagt gcgcttgttg ctttcgtggt
     5941 ggcctcgctc gcggcatagg tcactttgcg gatccaaggg gagccgctcc aggaggtcat
     6001 cgcgccggtg aacgagatga gcaggaaggc gcgggccgcg agggcggggt tgaacgggtt
     6061 gtacccgagt ccgccgaata cctgcttggc gatggtgatg gcgaagaccg agcccgcgac
     6121 cgccatccag gtcggcagag caggcggcaa gttgaacgct aacaacaggc cggtgaccgc
     6181 ggcactgaga tcggagaggg ttgaggcgcg gcgcatggct ttccggcaga gccattcggt
     6241 gagcagacag gtggcgacac aggttacggt gagtcgcagc gcatgccatc ggaaaaacca
     6301 gagcgatgcc gccagagccg gtaaaagcgc gatcagcacg tccagcatga tgcgcctcac
     6361 ggaggaaccc gcatggatat gcggcgaagg gctgatgatg aaaaggtctg gagaagcttc
     6421 gggtttcatt gggaaggggt ttcgggttgc gggtttcagg ttaggaggtt ttggcggcag
     6481 cgcggcgtgc catgatatgg gctttggcgc gtcgcatgtg atggaccagc ggacgatggg
     6541 cgggacagac aaaggcgcac gagccgcact cgatgcaatc catcacggcg aggggttctg
     6601 ccgcttcgat atcctccgcc tcgatgcact ggctgagttc cgccgggctc aggcgcatgg
     6661 ggcaggcgtc cacgcagcgt ccgcacgcga tgcaggcttg ggaggtgaag caggtggtgt
     6721 ttttggggga gagcagcagc aggcccgagg cggttttgcc gaccggtacc gcgaggttgc
     6781 tgaccgtgaa gcccatcatc ggcccgcctg agatcacctt ggacactttg cccgtgatcc
     6841 cgccgcaggc ggccaccaag tcactgtaca gtgcgccgca gggcacgagc agattcgcgg
     6901 gttgcgcgat ggcgtccccg gtgaccgtga tcacgcgccg gaccagcggc cgcccgcggg
     6961 ccacggcttc ggcgatcgcg aaggcggtgc tgacattctc caccacacac ccgacatcca
     7021 tcggcagacc gccgcgcggc acctcgcgcc cggtgaccga aaagatctgt tgcttttccg
     7081 agccctgcgg gtaacgggtt cggagcacgg tcacggccac atcgccatcc atgtcctgaa
     7141 agattttttc caaaacttcg atggcgtcgg gtttgttgtc ctcgattgcg acgcgcaggg
     7201 ttttcgcgcc gaggatgtgg cagatgatct ccgcgccgag gcggatgtcc gcggcgtgtt
     7261 ccagcatcat acggtgatcc gcagtcagat agggctcgca ctcggcgccg ttgaggatca
     7321 cggtgtcgat gggtttgtcg cgcggcgggg agagtttcac gcgtgtgggg aagcctgcgc
     7381 cacccatgcc gacgactccg gcgacaccga tgcgctcaat cagcgatttg gggtcggccg
     7441 tccgccactc cgggaggggc ggcaacgact cccacgtctg ctcggacgcg gtttcgatca
     7501 cgatggcatc acaggcgcgg cctgtcgcgg tgagcgttgg ttcgagcgcg accaccttgc
     7561 cggcggccgg cgcgtggacc ggcgcggaga tgagaccgtc ggcttcggcg aggagttgtc
     7621 cgcctataac cgtgctgccg gtctggacaa caggcttggc cgacgcgccg aggtgctgcg
     7681 acagaggcac gaccagccgc gcgggaagcg gcaattcgcg gatgggcgaa ccggcggcga
     7741 gttccttgtg gtaatcggga tggatgccgc cgtggaaggt gaaggatgaa gggatcgtgg
     7801 gcatggaaag aagggaggag tgtgggggtt gggagttagg agttgggagg gtctttgcgg
     7861 atgcagtggc ccggacattt agcgacaacc tcctcatggg tgaggggctg ggagtagtcc
     7921 accatcgcga ggaagccgtc catgctgatc gcgccgtcgg agagtttggc gcagatgctg
     7981 caaccgaggc aacctgtgcc gcagaccttc ttcacggcgg ggcctttgtc cttcgaactg
     8041 cagagcacat ggatctctgc ggcggcgggc acaagcttga tcagtcggcg cggacaggcc
     8101 gcgacgcact tgccgcaggc gatgcagcgc ttcttgtcga tcttggcgat gccgttgagc
     8161 atatggatcg cgtttaccgg gcagattcgg gcgcaggaac cgtatccgag gcatccgtac
     8221 gtgcaagcct tgtctccgcc cgcgatcgtc tgggcggagg cacaatccgc gatcccgttg
     8281 taggttgcgc ggcgtgtcgc ttccgtgttg tcgccgcaac agagcacgat cgcgacccgc
     8341 ttctccagga cggccacggt gcgaccgagc aaagccgcga tcgcttgtgc gcagtccggg
     8401 ccgccgggtg cgcagaggtt gcattccgcg gtgccgtcca caatcgcgcg cgcgtagtcg
     8461 gcacagcccg cgtaaccgca gccgccgcaa ttggcgcccg gcagcagttc ggtcacctga
     8521 tcgatgcgcg ggtcctgttt gacggtgaag tatttttcag cggcagccag tgctgcggca
     8581 ctcgcgagcc cgatgccgcc gacacacaag gttgaagtgg tgatgagatc catgaaaggg
     8641 ttttcagaag ggttatttga cgaggccgtt gaagcccatg aatgccaggc tcaggagtcc
     8701 ggcggtgacg agggagatgg ccaccccgcg gaaacagcgg ggcgggtcgg cgcggtcgat
     8761 tttctcgcgc agaccggtga aaatcagcag ggcaaaggcg aacccggttg cggcggcaaa
     8821 gccgaacagc acggatttga agaggccgta tcccagtttc atgttcagca cggccacgcc
     8881 gagcaccgcg cagttggtgg tgataagcgg caggaagatg ccgagcgcgg catgcagcga
     8941 cggggtgaaa cgtttcatca cgatgtcgat caactgcacc agcgcggcga tcaccaggat
     9001 gaaagcgagg gtctgtaaga atttgtccag tcccagcggg gccagcacat aggtttgcag
     9061 gcaccaggtg acggccgacg cgatggtgat cacgaagatc accgcgcccg acatgccgag
     9121 cgcggtgctg atccgcttgg aaacgcccag aaagggacag atgccgagaa aacggctcag
     9181 cacaaagttg ttgaccagaa tcgcgctgag aagtaacaga aggtattcca tgtccacatc
     9241 ctcaggtgag gaacaaagcc caaaaatatc aaatcccggt atggggttca atctctgcgt
     9301 gcgactcggc aactacaaaa ttacaagggt ttctggtttc agggtttcaa ggtttcaagg
     9361 tttctggttt cagggtttca gggtttcagg gtttctgggt ttcagggttt caaggtttca
     9421 gggtttcaag gtttctgggt ttcatggttt cagggtttca tccaaatcgg gatcggtatc
     9481 gggatcggta tcgggatcgg tatcgggatc gtcgacccgc gacattcgac ccgcgacgat
     9541 cgacagggac gcccgcgttt ccgacaccgc aggtcgcccg tcgcggggcg acataaggtc
     9601 tgacgggaga agcgggcgtc cctaccgttg gagcagggaa tacagttggg cgacgggctc
     9661 aatccttccg cgccgcattc gcgtcgtttc cggaacggta cgcgtcgagt gcttgccgga
     9721 acgcttcggt ctgcgcgtaa ccgcaactct tgaactcggg gcagaagccg cggtagacgc
     9781 attccggcac acagacgcgg tagagttcgg gttcgtgttc gcgcatcgcg tccagcacca
     9841 gacgccaggc ctcggtggtt tcgggggagg cttgcttaca gagccgtttg cgtgaaatga
     9901 agatcagggc ctgggcgttc gcgaagcact cgtggtcaac aaggcttgcc tgcggcatgg
     9961 tgtcgcggtt gacgccggta cggtccgagc gttgggtgct cacgaagtgt tcgattccga
    10021 ttttgtgccg cacgaagtgc acgctgaccc agtatttcag attgacccat ttccatttga
    10081 agagcatctg gcggatgggt gagtgttccg aaagcaggat gcggtgtttc caggcgttgc
    10141 tgggttcgcg gtcgccctcc tccatgcgga tggtggtgcg ggcggcgtcg gcgacatcgc
    10201 gccatacgga cttggttttg aagtatttca agatgggggg ctgactcatg cgacgttcct
    10261 ttcagtatgc gggtgaacga aaacgggtta ggggctcggg cacaggccac catccacgcg
    10321 caggatctgg cctgtgatcc aagcggcctc ttcggatgcg agaaaccgca cgactcccgc
    10381 aacttcgtcg ggtgtgccga aacggcctag cgggatggcg gcgagttgtt tgacgcgccg
    10441 ggtctcgggg atctcggcgg tcatgtccgt ggtgatgatg ccgggggcca cgacattcac
    10501 cgtgactccg gaggcggcca gttcgcgggc tgcggtcttg gcgagtccga acagggcggc
    10561 cttggaggaa ctgtaggccg cgcgcatgag atcgcccatc aggcctgcgg ccgaagcgat
    10621 gtagatgatc cggccggcct gttggcgcgc catggggcgc gtgagggctt tggttagcag
    10681 gaacgcggcg tcgaggttcg cggcgtgaac ctctcgccag gtgtcgagcc cggtgaaggc
    10741 gaggagctgg cttcgcagga tgccggcgtt gctgaccagg gtgtggatcc cgccgcaggt
    10801 tccgatgacc cgcttgacca gttcatccac ttctgcgggg acagtcaggt ctgcggcgaa
    10861 gggttcggct ttgccgccgg ctttgcggat cgccgcgaca gtctcttccg ccgcctcaac
    10921 cgaggtgtgg tagtggaggg cgatccggaa accgtcgtgc gcgagaccga gcgcgatcgc
    10981 gcgtccgatc ccttttgcag ctccggtgat gagggcggtt ttcatgcggg aagatatggc
    11041 gcgaggacgg tttcgtcaat cagatccgcg ccggtttcgg cccggacctt ttccagatcg
    11101 atgtgatgtt cgcgggctaa gcgccgggcg cgcggggtcg cgcggatacg cgtgccgcgg
    11161 tcggttcccg aaccggcgga agcggtgccg cggtacgctg cggtgagcgc ggcattcttt
    11221 tccggaatcg aggggtcctc ctcgccgggg gcaccgatga ggcctgcgat gaaaccggtc
    11281 ggcacgacgc tcttcgcggt ggcatagacc gcgagcagtg tgccggaggc gggggcttcc
    11341 agttcgtacg ctgccttgtc cgtggctagc tcggccacac attcgccggc ctcgaccgtg
    11401 tcgccgactt gtttgcgcca gcgcgagagg gtcacctcga ccacattcgc atcaagttgg
    11461 ggaattagaa tttgtgtcat aacggttttg aggttggggg ttaacggtta ggttggatca
    11521 gaagacaacg ggttccagtc ctgacagggc tggattgctg ttctgcgagc cgggcggcac
    11581 ggggatcatc cacgtgtcgg tcaggttccc gcggccgtct tgcaatcgga agcggaacca
    11641 gaggggatcg tcgggcggga gttgcaagtt cgcgggccgg ataccggtgg cctgcgagcg
    11701 tccgcctttc cattggaggc ccgcgacggt gctcaggtcg tacgcccgga tcggaatctg
    11761 caacgcggag tgccaaggcg agaggatgga gagggatcgg atttggacgg cgctcccttt
    11821 tgccttgtga cggaaaaaca acgagaggta cgtgatgtcc ggcgtgttct tgtgctcatc
    11881 ggtctcttgg agcagaaacg agcggacatc gatcagcacg tcattccaca cgccggtttg
    11941 caggttgagc gcgccggtga catacgccag atggttggtg tccttcagat tgagcgtgtg
    12001 cgcttccttg atgccgcggg gccgccgtgc gccggtgttg aacgcgaggg tgaaggggcg
    12061 gccctcccgc ggttcgccga ccgtgcggaa cgaaatcgcg agccaggggt agatatcggg
    12121 atcccaagtc ggcccgaagg tgtggcggat gtacgaggcg cgctcgccgt tcgccgtgat
    12181 ttcgagtgcc cgtccgtccg gtgtgacgcc ggtggtcgcc tgcacgtttt gcgattcggt
    12241 gaaaaagggg tcgagcgttg tcagggcggg ttcgcaccag agcatgtttg tcggaaaagc
    12301 ggtcggcagg atggtgggcg ggcgtgtgtc tttttcgagg ttgaccggga tctcgatttt
    12361 gacggggggc aaggcgttgc cggccgcatc ggtgatgccg ccgagcgaga gcggcaacgt
    12421 cgcgccgtgc ttgactgcgg cgagttgttc gcgcagcagc cagatccagt ccagctcgaa
    12481 tgcaatcttg ccttggccca cagtgcagaa cccgttgtct tgcttgaggt tgatcggggt
    12541 gccgaggtgg gtgagccgca gagtgttgag cactggcggt gccaccgggt cggtgagggt
    12601 gatcgtcagg cagctgccgt tatagtggtc cacgtcggtc agggcgtggt ggatttcggg
    12661 aggtttgcgg tcgaacagaa aggggacatc cgtaggttgg gaccaaaggc cttggccgtc
    12721 gcgcgcccgc accacaaggt ggtggatgcc gtcagccagc gcggtgaggt cgggttcaat
    12781 cactgcggcg ttcttgcccg gtagccaagc ggtcttttgc tgctcgtcgg ccgaccggtt
    12841 gtcccagacg gcaggccccg gaaggatggc gtaggcgatc tccgcgacac catccggatc
    12901 ggcgtagtcc gctttgaagg caaaggggcg gttcggtcct gcggcgggtc cgcaggtcag
    12961 gtcatcgatc cgcagtgtgc tgtgcagacc ggtctggtct ctcgtctgcc gcgaggccag
    13021 gcggatcggc gcgggcggca ggctgatgcg cttcgcgagg gggaagcggc ccacgctgtc
    13081 ggacgggatg ccggtccaga cacgccaggt gtcgtccatt tccgcgcgcg cgttatcggt
    13141 gaaccgcacg gaggtgttga acgcctctga gaacgtgaac gcgaactcgc caaaggcgag
    13201 cgaaacgatc gccatggggt cgcccttgta ccggaactgg agcagcggcg tggcggcgat
    13261 gtcgaaccgc gggaagagtt gtccggtgag gcgccgtccg tatacgcccg cgttgctgaa
    13321 ggcgagcaca ccgccgcgac ggggatcggt gacctccacc gcgacgcggg ccgccatctt
    13381 atggggggtc gtgtcgaatg ggcgggcctc aaaggtttca accgcgcctt ccggcatggt
    13441 gagcgaaagg aggaccggcg gggagttcac gctgtcctct ttccgcatgg ggacgacctg
    13501 acggaagaaa cgtccgtcat acagttccag cgcaagggtc atgcggcccg gctgcaaaat
    13561 attcagcggc acgagaatcc ggatcgtgtt gccttccgta taaaatggcg cgggctgcgc
    13621 atcgacttgc acttgcttgg gcgggagcat cggcgagggc caggggtggg tgggggttac
    13681 cacaatcgag ccgaggacag cggggtcgga ctgtgcgagc aggccgaatt tcgcccgttc
    13741 cggcacggcc cattcgatca caggtcggcg cgaatcgagg gctgcaggca gttcgaccat
    13801 ttgcagttcg ccggggctga ggccgtttat gagttgagtg agggaggcga tctcggcccg
    13861 tttttcgggc gggccgctca cggtcggaat cccgcggtgg atctcacgga aatgacggat
    13921 cgcataccag gcgtggggcg ggttgccttc cagcccttgt tgcacgtcgc tgggctggag
    13981 gttgccgaag ccttcgatct gcacggcctg attggcgcgg atgatttcgg agggcaccca
    14041 gatctcgacc ggggtccaga cccggtttgt gcctgcggga tcggatgcgg gcagggattc
    14101 aagggcaccg cagattttgc gcgggccgcg gctttcgttc gagccgcaga gaacgtaggt
    14161 ccagtgctgg atcggcgtcc agccgccttt gccgtcctcc ttgacggtgg cgaactcaaa
    14221 gttgacgcgg gcctgcggat gtcgcgcaat ctcgaagcgc catcccagca gcttgtcggg
    14281 ttgcgcgggc ggcagcatgc agcgcaccag gaaggagcct gcgccgagca gggaggtcac
    14341 atacatttcg ggacgggcgg tttggagatg gcggaaacgc acgttggggt ggctcacggt
    14401 gtcgtaagcc tgccagaaat ccggagtgag cggttgggcg acacggccat tgatacggat
    14461 gccggtgtcg gcgggagctg ctgcaggttc ggtggggggc ggtgccgcgg gaggaagcgc
    14521 ccggaaggcg aagggccggg gccggatgga ctccgcggcg atcttgatgc gtgcgaccat
    14581 catcgaattc cggtaggtcc agatgccaag cgccccttcc tgaagcggat cgggatcctc
    14641 gatgcggaag acatcctcgt tatcatagat gtagtgcagg gttttgccca cgcgccgcaa
    14701 ctgcatgccg taccaggcac cgtggatgtc gcggcccccg gccaccagcg gctggtagcc
    14761 ttggcgggcg aggcccgtgc gggcgcgcgg cacaaggtac ttggtggaga ggtcgagaag
    14821 gcttccgttc cggatcaggc gggagtagag ttgcgaatgg tccggatccc atccggtggc
    14881 aatcgcggtg tacccgtcgc cggttgagag agaacggctc atcatggtga ggttaaggtc
    14941 gcctggccgc gcataccagc ccatgcgcat ccccgcgtaa aattcgatgc tgaaatcgcc
    15001 gcggaagaca tatttggacc agagcgcagc gagcgagtca gcgctttccc cgttcatatg
    15061 cgaccaagta ggctcgcaat agaaccggtt gatgacctcc caccggccgc cgttgatcgt
    15121 ccagttgtag agggattccg tgaagagcgt gtcaaacacg ttgtcgcgtt tcaccagcgt
    15181 ccggctcaga ttgtccagcg tcatgccgct gatccgcatg cggcggccat gcaacggcgc
    15241 gtgcagatgg gtttgggcaa gcagaagggt gtcgccgccg agccagagga cggagccttc
    15301 cagtccgagg gtgaaaagac gcagcttggt gttgttgaac tcgacttcgg gcacctggtc
    15361 ggccgacacc tcgaaggccg tattcgttcc gctttccggt gtgaagacgc ggatgacccc
    15421 ttttttcaca gaaacgcggc acaggccgtt ggagagcccc tccggaacgc cgagatgcag
    15481 ttccatttcc tccacgaccg gcagccggac ccgcaccgcg ccggtgatgt cgcctttgaa
    15541 ccacgtcatg ccgtttttga aagtgatcca ttgaccttcc ggagaggccc agtgccgcat
    15601 gaacggatca ttgacataca aggggttttt ctcgaagcgc tcggagagtc ccgtctcacg
    15661 cgaggtgacg gtcggcgcgg tgaaaaaaag atcgccgggg gttacggcca gaacgccgag
    15721 ttcaccgccg ggctgatctt ccgggcgatg gaagcagacc agaaggtcgt cggcataggc
    15781 acgaagttcg tgcggccgca gggcgtccag agcgaggatc acccggttgc tggagaccgg
    15841 catttggaag gagtccagaa gaacggtgcc gttggttgtc acatgttcga gccggaaggt
    15901 gcggccgccc ccatcctgtt cacaggtgaa acggtatggg gccgcaccgt ctccggaacc
    15961 gcccgcggtc agaccgcaca ggaaggagtc ggtagtggca accacccgaa tctcctgccg
    16021 cagcgggccg tcgtcggaac tgccgtacgc ccacatttgc gtggttgtgg cggagggcgg
    16081 aaaatagagc catgccgtgt cgtgacgctg cagggagcgc acgtccgggc tttggcggcg
    16141 cgtggcaaag gtgacatcct cgggcgagtc gaaaaggcga tcctgatgcg atgccgcggt
    16201 cacatcatcg aaacgggtgg tttcccctgc gggcatgttt gcgaaaagcc cgaaacgtcc
    16261 gcctggcggc aggccgatct tatggcggat cacctcgatg ttgtcggcgc ggcaaaggat
    16321 acggtcgtca aacgtctgca cctcaagcag gagccattgg cctgcgggca gttcggtttg
    16381 cacgcccgcg agatacaggc ggggtgcgtt gggcgcggcc ggctgctgcc acaagtcgag
    16441 gatcagacgg tccgacaccg gatccgtgcg ggccgtgaag gcaaagtagc cgccgtgctc
    16501 gcccgccaag aaaaccagcc cgttggtgcc ggtgttgtgc tggactgcgg cacggaagac
    16561 ataacggctg tagaaaggtt cgcccgccag gacgatggcg ttggtcccgc cgccctccag
    16621 cgtgtagaag ttcgggctct tttccggttt cggttggcgc gccagttgat agccgccgga
    16681 cgagccgctg atcgaaccgg tgaccgaatg caggcgccag atgccgcttt ggatatccca
    16741 agtggggggg aaggccgaac cggccggaac caaaaagttg tcctcaaacg tgaaggagcc
    16801 gaggcgttgc gtgtactcat cggcctccgc ctctggcggc tggagcgccg gggggtgttc
    16861 cacgttcacc gctccctgcc agagttccgg cagacgcgcc accggccggt cgtccaccgt
    16921 gaggatccag acgtccgcat ggcgtttcag ggtgatccag tggagcggac cggcgagcga
    16981 cgggcacgga taagtggtga cgcggcgttc ggtcgcgtca tcgaagaacc cgcggggcaa
    17041 caggcggaag cgggtatccc cttcgcttaa cgtgaactcc gcctggcagg tgtgatccgg
    17101 atggctgagg cggagacgca acgcgccgcg ttccggcgtc agattcaggg caccccattc
    17161 cgaccgttcg gtcagatcca gacgggtgga ccacgcctct tcagccgcca accctgcggt
    17221 ggcggcgcaa cacccccatg tcagccccgc ccatatcaaa acgttcgtcc agttcacgcg
    17281 atttagttta acaggtttta caccggaggg gggagggaaa atgcggacgg aacgttcgga
    17341 tcgaagaggc agccggggtt gagccgtccc gcggggtcga acaaccgctt gatccgccgc
    17401 atcgccttca ggccttcggc tccgaccatc tcgcgcagca gttcgcgctt gagacggccg
    17461 accccgtgtt cggcggagat gctgccgccc caggcgacga cctgcgcagc ccatgagtgg
    17521 tagagacgtt tccccattac gtattcgtcc atattgcggg gcagaatgtt gacgtggaga
    17581 tggttgttgc cgatatgacc gaaaatgaca tgttgcagat tggctgccgt gagatcttcg
    17641 cggtaacgac gcagcagcgg acgcaacttg gcgtccggca cggagaaatc ggttccgagt
    17701 ttggtgaggc cgggatgcgc gcgcgcgcgt tccgcaatca aggcgttgat ctgttccggg
    17761 acggcatggc ggaaggtttt gagtcggccc agcgcaaccg tttcgtgcgc gacccaagtg
    17821 tcgacggcgt tgccgcccgc agcggcgacc agacgggcgg ttcggtccag agcagcatcc
    17881 ggtccgtcat tcaaagccca ctcgagatag accgcgtttc gtccgcggga cggcagggac
    17941 ggcaggggta cgccggtctg cgccgcacct tcgtgcgcca gaagcagagc ctccgcatcg
    18001 aaatactcga tggcggtgag gagaggcgag ggggccgccg cagcctgctg ccggatttgt
    18061 tccaccagcg tcagggcctg cggctcctca ttaaaaaacg cgacgatgcc gaggatgccc
    18121 gcaggcgcgg gtgagagacg cagttcgagt tccgcgagaa tggcgagcgt cccctcgctg
    18181 ccgagcagca ggtcgatcag atccatgtcg ggacgggccg cgtatccggc cgcatttttg
    18241 acctccggca caggtagaag aggcagatcc ccctccagac gacgcccgct ctccgtggtg
    18301 accgcgaatc ggcgccctgc ggcccgttcg cggccgcggc gcagcgtcag ggtgtcgccg
    18361 tccgccagga cggccgtgac cgcctcgatg tggcgccgcg tcgcgccgta ggcgaaggag
    18421 cgggcgccgg aggcgttgca agccgccatg ccgccgagag tggccgacgg ctcggtgggg
    18481 tcgggcggaa agaagagcgg gtcgggaaag agccgtcgat cggaaagatg gcgttggagg
    18541 agcacgagcg gcagccccgg ttcgacgcgc aggagggtcc ggtccggcgt ctcccggcaa
    18601 caggccagga tccggtccat cctcgaaaga ttgatcacca ggccgccatc aggcacggca
    18661 ccggccgcaa tcccggtgcg cgccccctgc ggggtgagcg gcaggcctgc cgtatccgcc
    18721 gcgcgcagga gggcggtcag ttcggagcgg tcgcgcggga aggcgatcgc ttcggccacg
    18781 ccggtcttgc gcgattcgtc ccggaggtat tcggcgggga ccggcttcgt caaccgtggc
    18841 gtataagaca tgacgccgat cataccggga cgcctcccga agccgcaagc gcaaagcgcg
    18901 cgcctttacg ggcaagcaga cggtccctgc cattttgatt tcatgaagtt tcaaggtttc
    18961 atccaaatcg ctatcgggat cgctatcggg atcgggatcg ctatcgggat cgggatcgat
    19021 tgtttaacca cgggacacac agaacacacg gaaaggcatg gctctgtttt cgtttgtttg
    19081 acgccgttcg tggtttcaat cagaagttgc gttttgcacc aaatcggtgc agcgcggatc
    19141 tcaccgaatt ttagggttaa tccttatttc gcgctctttg tctcacatgg ggcggcggcc
    19201 ttatagagtt cggccagctt gagcagggct ccgtccacga gacgttcggc ggtgcccgcc
    19261 ttgaagcggc tggtggcgtt ttcgtagcct ccggggccgt aggcggattt caccgggaaa
    19321 tagccgcgcg aaccgttgac ggcgcaagcg gggattgtca tcgcgaatgg cgactttgct
    19381 ttaagctccg tgcccatcca tgtgaagggt tctcccggga aaccgccaaa ggcgagcgtc
    19441 gtgccgaccg tcaccacgga aacccgcagg tcgacttcat cgggggcgtc cttgagttcg
    19501 aggatgcggt aagctccggc ggtattggct gtgcgttcca tgccgatgcc gggaatctcg
    19561 ttggcgcgtc ccgacttgtg caaacggacg tatttctcgg cgaggggcaa ctcttccggt
    19621 ttgcccttgt tcgtgaggac tttcactttg ggcattgccg catgcacctt gccggatggc
    19681 gcgggcgcgc acatccccca cgttgcaagc gcggcgccgg cgatcgtgcg ggccatgtgc
    19741 cttgacatct cgtagcgtct cggaacgatt tctccgggag ctgttttcgg acggaggtgg
    19801 ttggtgtcgc cctgtgcgcc gttgatcagc ag
//
LOCUS       DAOSRB010000026        19600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2946_length_19600_cov_6.686979, whole genome shotgun sequence.
ACCESSION   DAOSRB010000026 DAOSRB010000000
VERSION     DAOSRB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19600
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2946_length_19600_cov_6.686979"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..1789)
                     /locus_tag="PLZ74_02650"
     CDS             complement(374..1789)
                     /locus_tag="PLZ74_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97251.1"
                     /translation="MSFTRQRFLKTVLGAGAAFTGGCLGTRNAPPMRPARSAGRLRVA
                     LIGVGGQGLVNLRAVTDAADVAEPVALCDVDETVLLAARDRVTPQTSKVRLYKDFRVL
                     FDAERSLDAVFLSTPDHGHALQAAWALAAGCDLYIEPPAARTLGEVARMRMLARQQGA
                     LVQIGLRTATGEAFCRALEILDTGILGAVTVLHAWTNRPVWPQGNPRPEGSDIVPVGF
                     DWDLWLGGAPTRPYISRVYHPFNWRGWHDFGTGTLGDAGSDLLHLPFRALALQAPVTI
                     ESETAVPQTGESFPRASRLRFTFAARRRQPPVTLHWYDGGERPDAARLEPVTAAFGRL
                     PDAGCLLIGSRGLWFTSGDPTPRHFLALTGETSVREMTRHPACRDVPQCLPRTASPQR
                     EFLDALRNGTPTLSDLEHTSALTACILTGCVAQRIPGRLTWQARRNRFVNHVAANSLV
                     APVYREPWGEPENQIEERRTR"
     gene            complement(1786..3183)
                     /locus_tag="PLZ74_02655"
     CDS             complement(1786..3183)
                     /locus_tag="PLZ74_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97252.1"
                     /translation="MRNGLIPFLRQTGGTLRLAAHSMLRTRTIAAVLVLLAACVGLLP
                     RAVQGDGTPSGDIQILLQYTLGFAFFILCLATLWSACALLAAEIDSRRIHLTVVKPVR
                     PISLWLGRWLALLVIQAALLAVIYLGTYAQLHWRMRQDGWCPADRPASRRVTRPLLPS
                     PSEEARAILASMHANGTRPQGVSDAAILRTLETRAREKFDVVNPDQDVRWRFRLGRPL
                     QPGEPLTVRLRFDSEYSTREHVRGVCRIWPEAYPERVVEHRLNDFTLNEIALPVDTRA
                     FSDAAGHDPEGLRTFVLVFRHVGETRRVSALMLRFRQDVALLTAGGSFEANLARAALL
                     QWAVLALLAAFGLTLSACFSLPVAAFVATLTLVLTLLGHAVVRTVSVEEEQAWRNRVG
                     IWVSRGISSITSRAVQAEPLTSLTRGERIESDRIGETLIWNALLLPLGLAGMGVLMLR
                     HREWADTESCGKDHT"
     gene            complement(3168..4106)
                     /locus_tag="PLZ74_02660"
     CDS             complement(3168..4106)
                     /locus_tag="PLZ74_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR97253.1"
                     /translation="METRQVIETRGVRKTFRDFWRRPVVEAVKDLNLSVARGEVFGLL
                     GPNGSGKSTTLKMLLGLLRPTAGTIRVLDAPPQALQARARIGYLPEISHLHPFLTPRE
                     TLRYYASLFGLGRRETERRTEELLEMVDLTQAADRAVGRFSKGMARRVGLAQALMNAP
                     ELLILDEPTSGLDPLGARAVKDLVRTLAGAGVTVLMTSHLLADVEDVCDRVAILDCGA
                     LCAEGRTDDLLRQPDAVRFEIEGMDDAAAREARSALETRFGRPVRQSYPSLTLERYFL
                     QVVADGRTSDAFRLAPFLLGPQPDPEPSGGAPCATA"
     gene            complement(4155..5240)
                     /locus_tag="PLZ74_02665"
     CDS             complement(4155..5240)
                     /locus_tag="PLZ74_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97254.1"
                     /translation="MTVRSSSQHSLRRGGRQLLALAGTTVIESIQQPAALLMLLTAVV
                     TTLLVPVFQFHRFGEDGRLARDSGLSCMLIFGMALAVSSAGHAVAGELTRGTAAAVLG
                     KPVARPLFLLAKWVGVLGVVTLFWCAQLAATLLAERISAHFLIRGDTAGYASDPITLL
                     LALTGLAVALFIAAARHAVLRRRFGVSAFLGIPISQGIVVLISGFYNRFGQLYPLYGE
                     AACEGCGSAHTHETAQVWLYHAELNLRVIPAALLLLFVLAVFAALAVALATRLPTGSV
                     LTVCAMTLLLGLAGDALFANAARFSARGLLAGVLPDLQHFWLCDRIARGGSVPGRYVA
                     EAGLYALTCCALFLTAGSVAFRNRDLG"
     gene            5427..6128
                     /locus_tag="PLZ74_02670"
     CDS             5427..6128
                     /locus_tag="PLZ74_02670"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOR97255.1"
                     /translation="MRRRLTRAVFFISCVAALTGMRVAGQDTAPACDSSGVCTLPDTA
                     VTVARPAGGRQFWAEKRLFTEAPPLEVARWLTDPPETKGKFLVIEFWRTWCGACKRGT
                     PFMNGLQKKYGKELAVIGITGEPEEVVRAYKGPKKEYFLALDRPKTETAESGGQNAEN
                     PPVEGTIPREKGQGAYEALFGVWGWPHVVILEPRFRTVVWEGYPGQKGYELTEALIEK
                     LLAIERAARAESGNP"
     gene            6218..10141
                     /locus_tag="PLZ74_02675"
     CDS             6218..10141
                     /locus_tag="PLZ74_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOR97256.1"
                     /translation="MSHKGERIGVAFSVLLLAFAVRAEMTYYAHQGKISAVALVGEGK
                     SLGTGKVSKISSLGERKFGDSWNVEFSGELTVPAAGTYAFTLTSDDGSALFVAGKQVI
                     MNDGLHGPSKRTGKADLAAGKHQVSLVYFNFTGGHELKATVQPPNGPETDLAAACTPS
                     SVRHDFGELREQARQRLAEEARKAHERAEAAAQAALFEISSPEALVRSIRHLLKTKPR
                     DYKNGRAHLKRAEEFVRDLPALRERLKQGDEQAKNALLEFGKLKRQALVEDNPYLDFD
                     ETLVVVSDKVAVKANWVGTHTMAAKGYKNKLVRFNLRTGALTDVYAPTDGAYVGEPDL
                     HYDAQKLLFTSTDTNNMFQVMEVGLDGSNPRQVSTIVGEYVHNYGGIYLPDDKIVFSS
                     TAPMVGVPCIGGSTTVPNLYLMGPNGERPRQLTFEQDADWYPTVREDGQIMFLRWEYT
                     DIMHYYSRIMMTMNPDGSNQRAIYGTQSLWPNSMFYARPIPGKAGQFVAVVSGHHGVA
                     ASGKLTIFDTNKGHTHADGVVQHIPGYGKTVTHVTVDQLYPRVAPKLLKSFPELQKNV
                     DDLIAKHMPAESQQGKDYHELNNDFFNKCYPLLRGIYPEMALDLDTLVNGVYPQFLQP
                     YPLSANYFLTVAKLTGSAPWRLYLVDTFDNFVPLQPEKLDGYAYMVEPYPIRKRVRPP
                     VVPDRVNLQDKEAVCYVQNVYRGLGLKDVPVGTIDSLRIFTYAYGYYKIGSHDHIGVE
                     SGWDIKRLLGTVKVEPDGSAMFRIPANTTISLQPLDREGRAVQLFRSWLVAMPGEKLS
                     CIGCHEPPTEPPVTSKTIASGKPPQTIKPWRPRVDGFAFETEVQPVLDAYCVRCHDGS
                     NAKLPNFKGRAPDVPPRGYSEAYHAFHKYFRRPGPESASTMFIPYEYHMSTSEGAQLL
                     EKGHHGVKLDQESWLRLNAWVDLNVPYFGNWTSVYASNEKRHTWTTNISAYAASLRAQ
                     YANVHDDWESIPDQPYPVNVTRGKGEQRAAPVQVSAPQWPLTAEAAKALQQAQAGGNL
                     KRTIDLGNGLSITLVRIPAGEFVMGSDEETPWEQPRHRVRIARPFWISETEINNAAFF
                     TFKPDHNAGVFDQQWKDHVRLGYYSNYSEQPAVRMSWNDADAFCAWLGKKTGLKIALP
                     TEAQWEWACRAGSATPMSFGARDADFSPFANLADKSIEKFAVSGVNPVFRPNLVNNRV
                     FDFVPRVREIDDKQFLVSGTRQYQPNAWGVYDMHGNVAEWTRSDYVRYPYDAARSNSR
                     KPEVKKVVRGGSFYDRPYRATSSYRLGYVPWQGVFNVGFRVIIEE"
     gene            10188..12005
                     /locus_tag="PLZ74_02680"
     CDS             10188..12005
                     /locus_tag="PLZ74_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOR97257.1"
                     /translation="MARIAARWIGVAVALVYVQATAAETPLTPVDVVADRAGKTLYVA
                     CETAGKVLVFDVESEKVTARYAVEGVARLAVADEQDRIYATAGGARGLLHELDRKSGR
                     ILRTLPAGHTPEALCLSADGKTLYYCNRFARADQPNVHAVDLASWKVRNTAKAVREPC
                     ASCLSDDGSTLWVGNYLPLMPANAQHVYAVVEVYDAATLKKRATLNLPAGAFAVRDMV
                     MSRDGKTCFVSHSVGRFTVPTTHLDRGWINTSAVSIFDVAACRYVNSVLLDDTVKGAA
                     NPWGLGVVGQDAWLCVALSGTHEMMVVDLKEMWKRLAAAANPAEIVNDLSFLYGAKTR
                     VSLEGQGAQGLAVCGERVFAAMRYADSLNLVEIWEDGPGAAVPLPLGPEPKPDILRLG
                     EMAFNDATLCYQHWLSCASCHPETRSDGTNWDLMNDGIGNPKQSRSLLYSHRASPVMI
                     TGIRASAEVAVAKGFELIQFHELPQDRLDSVDAYIKAQEPVPSPYLNPDGSMTAAALR
                     GKKIFEGKAACVKCHTPPYHSDKKKYTFGLGSDSERDRPFVTPILIEVWRTAPYMYDG
                     RAVTIQDVITTDNVNNKHGNTRDLTPDEVEDLAEYVNSL"
     gene            complement(12047..13138)
                     /locus_tag="PLZ74_02685"
     CDS             complement(12047..13138)
                     /locus_tag="PLZ74_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOR97258.1"
                     /translation="MTTSFPFPFRPVDAPYFRIAVLCAVLAGGCSRQPAAPLRIEWLC
                     MGTVAAVQCADRSRATDARDQAQAVFAELDREFSAWHGESVLSAVNRAAGSGQATAVS
                     ACFATVLQTALDASEASGGAFNPMIGPVMQAWGFNGAMQPPEYPDDRILNAAFAMADW
                     RAVICNKTTDGAEVRLPVAGMSLDLGAIAKGYAVDLAWERLKAAGHTNLLIDLGGNLR
                     ALGEAAPGRGGWRTGVRNPFQSNTMVGKFLMRHGEAVATSGNYERFVEIQGLRYAHIL
                     DGRTGLPVTGMASVTVIAPDAMTADLLSTTLFILGPEAGVRLLSRYPGCEALWIPDTP
                     DQLTLLSTPGFADRLTPHGKTPYALSVIR"
     gene            complement(13135..18087)
                     /locus_tag="PLZ74_02690"
     CDS             complement(13135..18087)
                     /locus_tag="PLZ74_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOR97259.1"
                     /translation="MTVARNIVWHVAACVFALGARVQAADPGEACATQTLTFALPRVC
                     ITADGRTRVGLAGCESDAVTGLPVLPVAGVTFSVPPGMAVDRVQVLHDAVTEIPLDAP
                     VEWGQPPPRIGEMPFPEAPPDPAVYGGTHPYPDPEQTVWRVDPNGADAARLSVRVCPV
                     RYDPQRSLLLAAASVTVTVEFRALRPTGIAVRSAATATSELNEMPGPFTYVIVSTSNL
                     IHNTPAPWNFAALCAARARTGFTTTCVSAEWIDSRYAGENRADRIRAFVQDAHRNWGT
                     RFLLIGGTFDLIPVRKLRVAVNEIIVTRIADIPADALYYGCLDGPFDGNGNGLFGELQ
                     DGANGGDIDLLAEVYVGRFPVANPEELSRMIRKTLRYEAASAEELAPCAFLAEKMDLG
                     LMTYTGGFMEELRFGSTYDGLTTIGFETSAFAPALDTGHTLYDSDAGLWSTEHAFAFL
                     NRNFGILNHIGHAVAKQCMKINLYTSAHQAALQACTNTLPYFVYSQSCLAGAFDTPDC
                     FAEQLVTVSNAAFAAVMNAREGWVYNHVVGGFSHRFHRSFWDAAMRGNGTRLGEIGEC
                     ARRMNLHLVSPSYGTYWRWVFYELTLFGDPATPFAARVNPTPPVFDHPELINTYETQT
                     AHRVACSLEPIGLYDPASVRLLWRTGSDPETVHTQFLANVSGNRYETTIPAHPAGTAI
                     RYALFAETLAGVTGRWPLEEPEHLFHITDRLDLEIRGTPAAIGTPDPGYGTTHYASGL
                     VAVASAPEHVAVTEETRHTCIGFTGGGSTPPAGDQPDVAFRIDTSSHLTWIWQREHLF
                     SVTSALSEFPHTAFWVPENEASPVPPAPATLFVDGVLYAFAEWWCNDQRLPPAPAHSP
                     HDLGAIIADQPRRFTARYLPIEQDTDGNGIPDWWETHYYGHSGRLPDSDDDQDGYTLA
                     EEYADRTDPLRATSVPAPPVITHEPLAARQTHPAPFIVQARITDTHCVTSAVLYWCHN
                     TNLWQTVPMTAGSNDLFFAEIHAECAPGDTFEYRIAATDPTGRTAESGPYPFALFYPL
                     IDTVQLHDLMIVAQPTQTPFTVRMDLANRGNAELYWRVQPARIERILSERLPSWDFTS
                     LGQPWSVSTNRAFSPPYALHSRLLSNAQPRTPVRASLTFPAVTLEPGATLSFRYWIFS
                     EVHQNTTRAFDGGLVEISTDGGETFMLLRGPYTHTIYGWNASPWPEGTPCFAGKGTEG
                     WQTAVFDLAAHHPEANGFAGSNVVFRFHYGGDNNTDNEGWYLDDIAVSPIRWPAGFNF
                     YGAAPETRTLAPGAHYRLLWSTDPSAMTMRDNAVDILFDSNEPVHQDFSVRWQVILRE
                     FPNLPGFNAVQTSSGDGRVLLETGLADRDGEAVSLEVAWSPDNGGHWQPAALHDVTVS
                     EGDAADDAPDGCLAALPTSRAGQPVTNLFRAFWSSREAVPPVGVNTQTRFRIVADNGF
                     FQVTALTQRVTVDNLPPRFLPGMLSAAPTSPQGGYAVTENLLTLHWPAAEDEPADSLL
                     RYRLTSQCGTSILEQTSHTLALSNALDQSHAFQVVAFDPAGNTSVPLDATLLVLNAFS
                     DYDGDGVTTADEELAGTRADDPSDRFTVRLRESADALTLSWPGVVGRLYTIETTPSLA
                     PAVWQPLAGHIDIPGTGAMHDAVLPRTTPTSFYRIRITLP"
     gene            18222..19445
                     /locus_tag="PLZ74_02695"
     CDS             18222..19445
                     /locus_tag="PLZ74_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR97260.1"
                     /translation="MTRQLAVMLAVACLAGSGRSGDWSQWRGSNRDLIVTEERLLPEW
                     PVGGPQRLWHVELPGEGYSEPIVVGGTLYITGSSGEKKSRVGHLFALDPKSGAIRWQV
                     EYGPEWGASYEFARTSPTFHEGRLYLVGGLGHVVCIDAKKGDIVWRVDSHAEFSGRNI
                     TWGIADNPLIYDGKVICQPGGPDAAIVALDIKTGRAVWKSKGLSERSAYCSPALLTIN
                     GRRQVVTMLEDHVVGVDAATGQPLWRYPHRNKYAVHPNTPVLCGKDRVFISSGYVLGS
                     AILEIAGGTVKQIWADKSSDNHFQGVAFYKGRIFSSGGGRLTCFDPADGRAVYHVEGA
                     KKTSFCITPVGMITYDERGGTVMLVEVGADSHRVISSFKIEYGNGPHWSSPVVSDGVL
                     YLRRGKGLAAFAVGD"
BASE COUNT         3665 a         5847 c         6652 g         3436 t
ORIGIN      
        1 cggcagcgaa tcaaacagcc gccgctggtt cggcgtcttt cggacgtggt gatgctgttc
       61 atggaaaacg aaggtgaagg agatggttat gatttcaaaa catgattttc tgagaagcat
      121 ggggatcgga gtcgccgggc ttgcaactgg aaaggttgtg gggggatgaa cctgtcgctt
      181 cgaagtccga gctgtcggat tgtagacggc gaggttttcg aaccagccca tcggcttcct
      241 gtcttccacg agacggatgt cgttgttgta ggcggtggaa cggccggttt tgcaggcagc
      301 ttcggcgtcg cgagcgtacg ctcctgttcc gccagaagat ccttttcccg ccccatggtg
      361 tagcgatcaa ccgtcatctt gtccttctct cttcaatctg gttttccggt tcgccccacg
      421 gttcccggta gactggggcg accaggctgt ttgccgccac atgattcacg aaacggttgc
      481 gtcgtgcctg ccacgtcaag cgccccggga tccgttgcgc cacgcacccg gtcaggatgc
      541 aggcggtcag cgcggaggtg tgctccagat ccgagagcgt gggcgtcccg ttccgcagcg
      601 cgtcgaggaa ctcgcgctgc ggactcgcgg tgcgcggcag gcattgcggc acgtcccggc
      661 aggccggatg gcgggtcatc tcccgcacgc tcgtttcgcc ggtcagcgcg agaaaatgtc
      721 ggggcgtggg gtcaccggag gtgaaccaga gcccgcgcga tccgatcagc aggcagccgg
      781 cgtccggcag tcgaccgaat gccgccgtga ccggttccaa ccgtgccgca tcgggccgtt
      841 cgccgccgtc ataccagtgc agcgtcaccg gcggttggcg tcggcgcgcc gcaaaggtga
      901 accggagccg actggcgcgc gggaaacttt ctccggtctg cggcaccgca gtttccgatt
      961 cgatggtcac gggcgcctgc aacgcgagcg cgcggaacgg caggtgcagc agatcactgc
     1021 ctgcgtcgcc caacgtgccg gttccgaaat cgtgccagcc gcgccagttg aagggatggt
     1081 agacccgtga tatgtagggc cgcgtcggcg cgccgcccag ccacagatcc caatcgaaac
     1141 caacaggcac gatgtcactg ccctccgggc gcggattacc ctgcggccag accggccggt
     1201 ttgtccaggc atggagaacg gtgaccgcgc cgaggatgcc ggtgtccaga atttcgaggg
     1261 cgcggcagaa cgcctccccc gtggcggtcc gcaaaccgat ctgcacgagc gcgccttgtt
     1321 gccgtgccaa catccgcatg cgcgccacct cgccaagggt ccgtgcggcc gggggttcga
     1381 tgtacaggtc gcaacctgcg gccaaagccc aggcggcctg cagggcgtgt ccgtgatccg
     1441 gcgtgctgag gaagaccgcg tccagcgagc gttcggcatc gaacaatacc cggaaatctt
     1501 tgtagagacg cactttggac gtctgtggcg tcacacggtc acgtgcggcc agcagaacgg
     1561 tctcgtccac atcgcagagc gccacgggtt cggccacatc ggccgcatcg gttaccgccc
     1621 gcagattgac cagtccctgt ccgccgactc cgatcaaggc gacgcggagc cgaccggcgg
     1681 atcgcgcagg ccgcatgggc ggtgcgttcc gcgttccgag acagccgccc gtgaatgcgg
     1741 cgcctgcgcc caaaaccgtt ttcagaaacc tctgacgggt gaaactcatg tgtggtcctt
     1801 tccgcatgac tccgtgtctg cccattcccg gtggcgcaac atcagcactc ccattcccgc
     1861 cagacccaag ggcaacagaa gtgcattcca gatgagggtt tcaccgatcc ggtccgattc
     1921 gatacgctcg ccgcgggtca gtgaggtcag gggttcggcc tgcaccgccc ggctcgtgat
     1981 ggaactgatg ccgcgcgaga cccagatgcc gacccggttc cgccatgcct gttcctcttc
     2041 aacggaaacc gtacgcacaa ccgcgtggcc cagcagggtc agcaccagcg tcagggtcgc
     2101 gacaaatgcg gctaccggca gcgagaaaca ggcgctcagg gtcaggccga aggctgccag
     2161 caaagccagc acagcccact ggagcagggc ggcgcgcgcc agattggcct caaacgatcc
     2221 gcctgccgtc agcaatgcca catcctgccg gaaacgcagc atcagggccg agacgcggcg
     2281 cgtctcgccg acgtgccgga aaacgaggac gaaggtgcgc aggccttcgg gatcatgacc
     2341 ggccgcatcg gaaaaggcgc gggtgtccac ggggagggcg atttcattca gggtgaaatc
     2401 gttgaggcga tgctccacga cccgttccgg atacgcctcc ggccagatgc ggcacacgcc
     2461 gcggacatgc tcgcgtgtgc tgtactccga atcgaaacgc aaccggacgg tcaggggctc
     2521 gcccggctgg agcggacggc cgagccggaa acgccaccgc acatcttgat cggggttcac
     2581 cacatcgaat ttttcgcggg cccgggtttc aagcgtgcgc aggattgcgg catcgctcac
     2641 accttggggt cgggttccgt tggcgtgcat ggaggccagt atcgcgcgcg cctcctcgct
     2701 cggcgacggc agcaagggtc gcgtcaccct tcggctggcg ggccggtcgg cagggcacca
     2761 gccgtcctgt cgcatgcgcc agtggagttg ggcgtaggtt ccgagataga tcacggccag
     2821 caacgctgct tggatcacga gcagggccag ccatctgccg agccagaggc tgatgggccg
     2881 caccggtttg acgactgtga ggtggatgcg gcggctgtcg atttcggcgg caagaagcgc
     2941 gcaggccgac catagtgtgg cgaggcagag gatgaaaaag gcgaacccca gcgtgtattg
     3001 gagcagaatc tgaatatcgc cggagggcgt gccgtcacct tgaacggcac ggggcaacaa
     3061 tcccacgcag gccgccagca acaccagcac cgcggcgatc gtgcgggtac ggagcatcga
     3121 gtgcgcggcc agccgcaggg tgcctccggt ctgccggaga aacgggatca ggccgttgcg
     3181 catggcgcgc ctccggacgg ttcggggtca ggttgcggac cgagcagaaa aggggccaga
     3241 cggaaggcat ccgacgttct tccatccgcc accacctgca ggaagtagcg ctccagcgtg
     3301 agggaggggt aactttgccg aacggggcgt ccgaaacgcg tctccaacgc actgcgcgct
     3361 tcgcgggccg cggcgtcatc catcccttcg atctcgaacc gcaccgcatc cggttggcgc
     3421 agcaggtcgt cggtacggcc ctccgcgcac agcgcgccgc agtcgagaat cgccacacgg
     3481 tcgcagacgt cctccacatc cgccagcaga tgggaggtca tcaagaccgt cacgcccgcc
     3541 ccggccagag ttcgcacgag atccttgacc gcgcgtgcgc cgagcggatc gagtccggag
     3601 gtcggttcgt ccagaatcag caattccggc gcgttcatca gcgcctgggc caaccccacg
     3661 cggcgtgcca ttcccttgga gaagcggccc acggcccggt cggccgcttg cgtcagatcc
     3721 accatctcca gcaactcttc cgtgcgccgc tcggtctcgc ggcgtccgag tccgaacagg
     3781 ctggcgtaat agcgcagcgt ttcgcgtggg gtcagaaacg gatgcaggtg cgaaatttcc
     3841 ggcaggtatc cgatccgcgc ccgggcctgc agcgcctggg gcggcgcgtc caaaacacgg
     3901 atcgtgccgg cggtggggcg caacagaccc agcagcattt taagggttgt gcttttgccg
     3961 gatccgttcg ggcccagtag cccgaagacc tcgccccgcg caaccgagag gttcaggtct
     4021 ttcaccgcct cgaccaccgg ccgccgccag aagtcgcgga acgttttgcg cacgccgcgc
     4081 gtttcgatga cttgtcgcgt ctccatgtgc ctcactccgt ttcgcagacc cccgcgcgcc
     4141 gcgcgtcctg cggttcatcc caaatcccgg ttgcggaacg ccacactgcc cgccgtcaga
     4201 aacagggcac agcaggtcag cgcgtagagg ccggcttcgg ccacataacg tcccggcacg
     4261 ctgccaccgc gcgcgatccg gtcacaaagc cagaaatgct gaagatcagg cagcacgcct
     4321 gccagcaggc cgcgtgcgga gaaccgcgcc gcgttcgcga acagcgcgtc acccgccagg
     4381 ccgagcagca gggtcatggc gcagaccgtc aatacggaac cggtcggcag gcgtgtcgcc
     4441 agagccacgg ccagcgcggc gaatacggcg agaacgaaga gcaggagcag ggctgcgggg
     4501 atcacgcgca gattgagttc cgcgtggtag agccaaacct gcgcggtttc gtgggtgtgc
     4561 gcgctgccgc agccttcaca ggccgcttcg ccgtataacg gatagagttg accgaaccgg
     4621 ttgtagaaac cggagatcag cacgacgatc ccctgcgaaa tcggtatgcc gagaaaggct
     4681 gacacgccga accggcggcg cagcacggcg tggcgcgccg cggcgatgaa gagcgccacc
     4741 gcgagcccgg tgagcgccag tagcagcgtg atcgggtccg aggcgtatcc tgcggtgtcg
     4801 ccccgaatca gaaaatgggc ggaaatgcgc tcggcgagca gcgtggccgc cagttgggcg
     4861 caccagaaga gcgtcaccac gccgagcacg cccacccatt tggccaagag gaaaagggga
     4921 cgcgccaccg gcttacccag cacggccgcc gcagtgccgc gtgtcagctc gcccgcgacc
     4981 gcgtgcccgg cgctgcttac ggcgagtgcc atcccgaaga tcagcatgca ggagaggccg
     5041 ctgtcccgcg ccagccggcc gtcctcgccg aaccggtgga actggaagac cggcacgagc
     5101 agtgtggtca ccactgcggt caggagcatc agcaacgcgg ccggctgctg gatcgactcg
     5161 atcacagtgg tccctgccag cgccaacagt tgtcggccgc cgcgccgcaa gctgtgctga
     5221 gaggaggaac gtacggtcat aaaaatcagt ttacccgaag ctgtcccgcg cgtcaaagtc
     5281 ggataagggg tgtacggcgc aatccgaaat gtgttatcct gtcctttatc tacaccccgc
     5341 attgttttgg gaaaccagcc atgcttgggc aggcgtccgt gtcgttgggg gtacgggcgg
     5401 agtagtcagg gaaaggaaga ccggagatga gacgccgttt gactcgtgcc gtatttttta
     5461 tcagttgcgt cgctgcgctg acgggtatgc gcgtcgcggg gcaggatacc gcgccagcgt
     5521 gcgacagctc gggggtctgc acgcttccgg acacggcggt tacggtcgcc cggccggccg
     5581 ggggtcggca gttctgggcg gagaaacggt tgttcaccga ggcgccgccg ctggaggtgg
     5641 cgcgctggct tacggacccg cccgagacca aaggcaagtt tctggtgatc gagttctggc
     5701 ggacctggtg cggcgcgtgc aaacgcggca cgccgttcat gaacgggctg cagaagaaat
     5761 acggtaagga gctggccgtg atcggcatca ccggcgagcc tgaagaagtt gtccgcgcct
     5821 acaaagggcc gaagaaagag tatttcttgg cgctggaccg tccgaagacc gagactgcgg
     5881 agagcggggg ccagaatgcc gaaaacccgc ctgtcgaggg gacgattccg cgcgagaagg
     5941 ggcagggcgc gtatgaggcc ctcttcggtg tgtgggggtg gccgcatgtc gtgatcctcg
     6001 aaccccgttt tcgtacggtg gtgtgggagg ggtatcccgg gcagaaaggt tacgagctga
     6061 ccgaggccct gattgagaag ttgctcgcga tcgagcgtgc ggcgcgggcg gaatccggca
     6121 atccgtgagt cctgtttcga agcttgccca aacaggccgg atggattaag atcaatttat
     6181 tttcaagccc tgcggggcgg aattcagtgg aggggatatg tcgcataagg gggaacggat
     6241 cggagtcgcg ttttcggtct tgttgttggc gtttgccgtc cgggccgaaa tgacgtatta
     6301 cgcacatcag gggaaaatct ctgccgtcgc gctggtgggg gagggaaaat cgctcggcac
     6361 cggcaaggtt tcgaagatca gttctttggg cgagcgtaag ttcggcgact cgtggaacgt
     6421 cgagttctcc ggcgaattga ctgtgcctgc agcggggacc tatgccttta ccctgacctc
     6481 ggatgacggc agcgcgctgt ttgtggccgg taagcaggtg atcatgaacg acgggttgca
     6541 cggaccctcg aagcggaccg gcaaagcgga tctcgcggcc ggcaagcacc aggtctcgct
     6601 ggtctatttc aacttcaccg gcggacatga gttgaaagcc acggtgcagc cccccaacgg
     6661 cccggagacg gatctggcgg cagcctgcac gccgagttcc gtccgccacg atttcggcga
     6721 gttgcgggag caggcccgtc agcgcttggc ggaagaggcg cgcaaggccc atgagcgcgc
     6781 ggaggccgcg gcacaggcgg cgctgttcga gatctcatcg cccgaggcgc tggtgcgttc
     6841 catccgccat ctcctgaaaa caaaaccgcg cgactacaag aacggccgtg cccatctcaa
     6901 gcgggccgag gagttcgtgc gggacctgcc cgcgttgcgg gagcgtctca agcagggcga
     6961 tgaacaggcg aaaaacgccc tgctggagtt cggcaagctc aagcgtcagg cactggtgga
     7021 ggacaacccg tatcttgatt tcgacgagac gctggtggtg gtgtccgaca aggtggcggt
     7081 caaggccaac tgggtgggga cgcacaccat ggcggccaag ggctacaaga acaagctggt
     7141 ccggttcaac ctgcgcaccg gcgccctgac cgatgtgtac gcgcccacgg acggcgcgta
     7201 tgtcggcgag ccggacctgc actatgacgc gcaaaaactg cttttcacct caacggacac
     7261 caacaacatg tttcaggtga tggaggtggg gctggacgga agcaaccccc gccaggtctc
     7321 gaccatcgtc ggggagtatg tgcacaacta cggcgggatc tatctgccgg acgacaagat
     7381 cgttttcagt tcgaccgcgc cgatggtcgg cgtgccctgt atcggcggtt caacgaccgt
     7441 gccgaacctg tacctcatgg gccccaacgg cgagcggccg cgccaactga cctttgaaca
     7501 ggatgcggac tggtatccga ccgtgcgcga ggacgggcag atcatgtttt tgcgctggga
     7561 gtatacggac atcatgcact actactcccg catcatgatg accatgaatc cggacggcag
     7621 caaccagcgg gcgatttacg ggacgcagag cctgtggccc aactccatgt tctatgcgcg
     7681 tccgatcccc ggcaaggcgg gccagttcgt ggccgtggtc tccgggcacc acggcgtcgc
     7741 cgcgtcgggc aagctgacga tcttcgacac caacaagggg cacacccacg ccgacggtgt
     7801 ggtgcagcac atccccggat acggcaagac cgtgacccat gtcactgtgg accagcttta
     7861 tccccgcgtc gcgccgaagc tgctgaagag cttcccggaa ttgcagaaga acgtggatga
     7921 cctgatcgcg aaacacatgc ctgcggaatc gcagcaaggc aaggattatc acgagttgaa
     7981 caatgacttc ttcaacaagt gctatccgct gttgcgcggc atctatcccg agatggcgct
     8041 ggatctcgac acgctggtga acggggtgta tccgcagttc ctccagccgt atccgctcag
     8101 cgcgaactac ttcctgaccg tcgccaaact caccggttcg gcaccgtggc ggctctatct
     8161 ggtggacacc ttcgacaact tcgtgccgct acagcccgag aaactggatg gctacgcata
     8221 tatggtcgag ccctatccga tccgcaagcg cgttcgtccg ccggtggtcc ctgaccgcgt
     8281 gaatctgcaa gacaaggagg cggtctgtta tgtccagaac gtctaccgcg gcctcggact
     8341 caaggatgtg ccggtcggaa cgattgactc gctgcgcatc ttcacatatg cctacggata
     8401 ttacaagatc ggcagccacg accacatcgg tgtggagagc ggctgggata tcaagcgcct
     8461 gctggggacc gtcaaggtcg agccggacgg gtcggccatg ttccgcattc cggccaacac
     8521 gacgatctcg ctccagcctc tggatcggga gggccgggcg gttcagctct tccgcagttg
     8581 gctggtggcc atgcccggtg aaaagctttc gtgcatcggg tgtcatgagc cgccgaccga
     8641 gccgccggtg accagcaaga cgatcgcctc cggcaagccg ccgcagacga tcaaaccctg
     8701 gcgtccgcgc gtggatggct tcgcgttcga gaccgaggtc cagccggtgc tggatgccta
     8761 ttgcgtgcgt tgccatgatg gcagcaacgc caaactcccg aatttcaagg gacgtgctcc
     8821 ggatgtacct ccccgcggtt atagtgaagc atatcatgcg ttccacaagt atttccgccg
     8881 tcccggtccc gagagtgcca gcacgatgtt catcccgtat gagtatcaca tgtccacctc
     8941 cgagggcgcg caactgcttg aaaaggggca tcacggtgtg aaactcgatc aggagtcctg
     9001 gttgcggctc aacgcatggg tggatctgaa tgtgccgtat ttcggaaact ggacctcggt
     9061 ctacgcctcc aatgagaaac gccatacttg gaccaccaac atctccgcgt atgcggcgtc
     9121 gctgcgggcg cagtacgcga acgtgcacga cgattgggag tccattcccg accagccgta
     9181 tccggtcaac gtgacccgcg ggaaggggga gcagcgtgcc gcgccggttc aggtttcggc
     9241 gccgcagtgg cccttgaccg ccgaggccgc gaaagcgctt caacaggcgc aggcgggcgg
     9301 taacctgaaa cggacgatcg acctcgggaa cgggctcagc atcacgctgg tccgcatccc
     9361 ggctggcgag ttcgtgatgg gcagcgatga ggaaacgcct tgggagcagc cgcgtcaccg
     9421 cgtccggatc gcgcggcctt tctggatttc cgaaacggaa atcaacaacg cggctttttt
     9481 caccttcaag ccggaccaca acgccggtgt gttcgaccag cagtggaagg accatgtgcg
     9541 actcggctac tactcgaatt attcggaaca gcccgcagtg cgtatgagtt ggaacgacgc
     9601 cgatgcgttc tgcgcgtggc tcggcaaaaa gaccggcctg aagatcgccc tgccgaccga
     9661 ggcgcaatgg gagtgggcct gccgcgcggg cagcgcaacc ccgatgtcct tcggcgcgcg
     9721 ggatgcggac ttcagcccgt tcgccaacct cgcggacaag tcgatcgaaa agttcgcggt
     9781 gagtggtgtg aacccggtgt ttcgtcccaa tctggtcaac aaccgcgtgt tcgactttgt
     9841 gccgcgtgtg agggagatcg acgacaagca gtttctggtc tccggcaccc ggcagtacca
     9901 gccgaacgcg tggggcgtgt atgacatgca cggcaacgtg gcagagtgga cgcgcagcga
     9961 ctatgtgcgg tatccgtacg acgcggcccg gtccaactcc cggaaaccgg aggtgaagaa
    10021 agtcgtgcgc ggtggctcgt tctatgaccg cccctatcgt gcgacatcca gctaccggtt
    10081 gggatatgtg ccgtggcagg gggtgttcaa tgtcgggttc cgcgtcatca tcgaggagtg
    10141 atgcggcagg gggagagatc atgcggggga tggggataga tggaaagatg gcgcgcattg
    10201 ccgcgcggtg gatcggggtg gcggttgcgc tggtatacgt gcaggcaacg gctgcggaaa
    10261 cgcctttgac gcctgtggat gtggtggcgg accgggccgg taaaaccctg tatgtggcat
    10321 gtgagaccgc cggcaaggtg ctggtgttcg atgtcgaatc ggaaaaggtc accgcccgtt
    10381 atgcggtcga aggggtcgcc cggctggctg tcgcggacga gcaggaccgg atctatgcca
    10441 ccgcgggtgg tgcccggggt ctgctgcatg agttggaccg gaaaagcgga cggatcctcc
    10501 ggacgcttcc tgcaggccac acgcccgagg cgctgtgcct cagtgcggat ggcaaaacgc
    10561 tctattactg caaccggttc gcgcgggccg accaaccgaa tgtgcatgcc gtggatctcg
    10621 cttcgtggaa ggtccggaac accgccaaag ctgtgcgcga gccatgcgca agctgcctgt
    10681 cggatgacgg ttccacgctg tgggtgggca actatttgcc gctgatgcct gccaacgcgc
    10741 agcacgttta cgccgtggtg gaagtgtatg atgccgcgac cctgaaaaag cgtgcgacgt
    10801 tgaacctgcc cgccggcgcc ttcgccgtcc gggatatggt gatgagccgg gacggcaaaa
    10861 cctgcttcgt ctcgcattcg gtcggacgct tcacggtgcc caccacgcat ctcgaccgcg
    10921 gatggatcaa taccagcgcc gtgtcgatct tcgatgtcgc ggcatgccgg tatgtgaatt
    10981 ccgtgttgct ggacgatacg gtgaagggcg ccgcgaaccc gtgggggctt ggtgtggtcg
    11041 gtcaggacgc ttggctgtgc gtggcccttt ccggcacgca tgagatgatg gttgtcgatc
    11101 tcaaagagat gtggaagcgt ttggccgctg ccgcgaaccc ggccgagatc gtcaatgacc
    11161 tcagttttct ctacggcgct aaaacgcgcg tgtctctgga gggtcagggt gcgcagggat
    11221 tggccgtctg cggcgagcgg gtctttgccg ccatgcggta tgccgacagc ctgaatctgg
    11281 tggagatttg ggaggacggt ccaggggccg ctgttccgtt gccgctcggt ccggaaccca
    11341 agccggacat cttgcggctc ggtgagatgg cgtttaacga cgcgacgctc tgctaccagc
    11401 attggctgag ttgcgccagt tgtcatccgg aaacacgttc ggatggcacc aactgggatt
    11461 tgatgaatga cgggatcggc aacccgaagc agtcgcgcag tctgctctat tcgcaccggg
    11521 cttcgccggt gatgattacg ggcatacgcg cctccgccga ggtggcggtg gccaaggggt
    11581 tcgagctcat ccagttccac gagttgccgc aagacaggct ggactcggtg gacgcctaca
    11641 tcaaagcgca ggagccggtg ccgagtccgt atctcaatcc cgatggatcc atgaccgccg
    11701 cggcgttgcg cggcaagaaa atctttgagg gtaaggccgc ctgcgtgaag tgccacacgc
    11761 cgccttatca cagcgacaag aagaagtata cgttcggact cggctccgac agtgagcgtg
    11821 accgtccctt tgtgacgccg atcctcatcg aggtgtggcg gaccgcgccc tacatgtacg
    11881 acggccgcgc ggtgacgatt caggacgtga tcacaaccga taacgtcaac aacaaacacg
    11941 ggaacacccg ggacctgacg ccggatgaag tcgaggacct ggcggagtat gtgaactcgc
    12001 tctgacaccg ggtcggatcc gcgcgccgcg tcaaacgacg gtcgccttac cggattacgg
    12061 agagcgcgta cggtgtcttg ccgtgcggcg tgagccggtc ggcaaatccg ggggtgctca
    12121 acagggtgag ctggtctggc gtgtcgggaa tccagagtgc ctcacagccg ggataccggc
    12181 tcagcagccg tacccccgct tccggaccga ggataaagag ggtggtggag agcaggtctg
    12241 cggtcatggc gtccggcgcg atcaccgtga cgctcgccat gccggtgacc ggcaggccgg
    12301 tgcggccatc cagaatgtgg gcgtagcgga ggccttggat ttcgacaaac cgctcgtaat
    12361 tgcctgaggt ggcgaccgcc tcgccgtggc gcatgagaaa cttgccgacc atcgtgttgg
    12421 actgaaaggg attgcgcacg ccggtgcgcc agccgccgcg ccccggcgca gcctcgccaa
    12481 gtgcgcgcag attgcccccg agatcgatca gcagattggt gtgccctgcg gctttgagac
    12541 gctcccatgc caaatccacc gcgtagccct tggcgatggc cccgaggtcc agcgacatgc
    12601 ccgccacggg caaacgaacc tctgccccgt cggtcgtttt gttgcagatc acggcgcgcc
    12661 agtccgccat cgcaaaggcc gcgttcagta tgcggtcgtc cggatactcc gggggctgca
    12721 tcgcgccgtt gaatccccag gcctgcatca ccggtccgat catcgggttg aacgcgccgc
    12781 cactggcttc cgaggcgtcc agcgcggttt gcagcacagt cgcgaaacaa gcggagacgg
    12841 cggtcgcttg gccgctgccg gctgcgcggt tgaccgccga gagcacgctt tccccgtgcc
    12901 aggcggagaa ttcacgatca agttcggcga acaccgcctg cgcctgatcg cgcgcgtccg
    12961 ttgcgcggct ccggtcggca cactgcactg cggcgaccgt ccccatgcag agccattcga
    13021 tgcgaagggg cgcggcaggc tgcctgctgc agccgccagc cagcacggcg cacaggacgg
    13081 caatccggaa atacggggca tcaacgggac ggaaagggaa gggaaaactc gtcgtcatgg
    13141 caatgtgatc cggattcggt aaaacgaagt tggggtggtg cgcggcaaaa cggcgtcgtg
    13201 catcgcgccg gtgccgggga tgtcaatgtg cccggccagc ggttgccaaa cggcgggggc
    13261 tagcgaggga gtggtttcga ttgtgtagag ccgcccgaca acgccgggcc aggagagcgt
    13321 gagtgcatcg gcggactcgc gcaaccggac ggtgaaacgg tcggacgggt cgtcggcacg
    13381 ggttcccgcc agttcctcgt ccgccgtggt tacaccgtcg ccgtcataat cgctgaacgc
    13441 attcagcacg aggagggtgg cgtcaagagg aacgctggtg ttgcctgccg gatcgaacgc
    13501 gaccacttgg aacgcatgcg actggtcgag cgcgttcgag agcgcgagcg tgtgggaggt
    13561 ctgttccaga atcgaggtgc cgcactggga ggtgagacgg taccggagga gggaatccgc
    13621 aggctcgtct tcggctgcgg gccagtgcag cgtcaggaga ttctcggtca ccgcgtagcc
    13681 cccctgcgga cttgtcggcg cggcactgag catgcccggc aggaaccggg gcggcaggtt
    13741 gtccaccgtc acacgctggg tcagggcggt cacttgaaag aacccgttgt cagccacgat
    13801 gcggaaccgg gtttgggtgt tgacgccgac aggcggcacc gcttcccgcg aagaccagaa
    13861 ggcgcggaag aggttggtca ccggttgtcc cgcgcgggag gtcggcaacg cggcaagaca
    13921 gccgtccggc gcgtcatcgg cggcatcgcc ttcagatacg gtcacatcat gcagcgcggc
    13981 gggttgccag tgccccccat tatccgggga ccacgcgacc tcgagactga ccgcctcgcc
    14041 gtcacggtcc gcgagaccgg tttccaaaag tacgcggccg tcgccggacg aagtctgcac
    14101 ggcgttgaat ccgggcaggt tcggaaattc gcggaggatc acctgccagc gtacgctgaa
    14161 atcctgatga accggctcgt tggaatcaaa aaggatatca acggcgttgt cgcgcatggt
    14221 catggccgat gggtcggtgg accaaagcag gcggtagtgt gcgccgggcg ccagagtgcg
    14281 ggtctccggg gcggcaccgt aaaagttgaa gcccgctggc cagcggatcg gggacacggc
    14341 gatatcgtca agataccagc cctcgttgtc ggtgttgttg tcgccgccgt agtggaaccg
    14401 gaacaccacg ttgctgcccg cgaaaccgtt cgcctcggga tggtgcgcag ccaggtcaaa
    14461 aactgcggtc tgccatcctt ccgtcccttt gcccgcgaaa cagggcgtgc cctcgggcca
    14521 gggagaagcg ttccagccgt aaatcgtgtg ggtatagggc ccgcgcagca gcatgaaggt
    14581 ctcgccgccg tccgtggaga tttcgaccag accgccgtcg aacgcccggg tggtgttttg
    14641 gtgaacctcg gagaagatcc agtaccggaa cgagagcgtg gcgccgggct ccagagtcac
    14701 tgcggggaag gtgaggctcg cgcgtaccgg ggtccggggt tgtgcgttgg aaagcagacg
    14761 gctgtggagc gcgtaggggg gcgagaacgc gcggttggtg gagacgctcc agggttggcc
    14821 gagtgaggtg aaatcccagg agggcaaacg ctcggagagg atgcgttcaa tccgtgcggg
    14881 ctgcacgcgc cagtagagtt cggcgttgcc ccggtttgcg agatccatgc gcacggtgaa
    14941 gggcgtctgg gtcggttggg ccacgatcat gagatcgtgg agttgcacgg tgtcgatcag
    15001 cggatagaac agggcgaagg ggtacggtcc cgattcggcg gtgcgtccgg tcggatcggt
    15061 ggcggcaatc cggtattcga acgtgtcacc gggcgcgcat tccgcgtgga tctcggcgaa
    15121 gaacagatcg ttggaaccgg cggtcatggg gacggtttgc cacaggttcg tgttgtggca
    15181 ccagtagagg actgcggagg ttacgcaatg ggtgtcggtg atgcgcgcct gcacgatgaa
    15241 gggcgcggga tgcgtctgcc gggccgcaag cggctcatgc gtgatgaccg gcggtgcggg
    15301 cacgctcgtc gcgcgcaggg ggtcggtacg gtccgcgtat tcctcggcca gcgtgtagcc
    15361 atcctgatcg tcgtccgaat ccggcagacg accggagtgg ccgtaatagt gcgtctccca
    15421 ccagtccggg atcccgttgc cgtccgtatc ctgctctatc ggcagataac gtgcggtgaa
    15481 ccggcggggt tggtccgcga tgatcgcgcc gaggtcatgg ggactgtggg ccggggccgg
    15541 gggcaggcgt tgatcgttgc accaccattc ggcaaaggcg taaagcacgc cgtcgacgaa
    15601 aagcgtggcc ggcgcgggtg gtacgggcga cgcctcgttc tccggcaccc agaaggccgt
    15661 gtgcggaaac tcggataacg cgctggtcac ggaaaacagg tgttcgcgct gccagatcca
    15721 ggtcaggtgc gaggaggtgt cgatacggaa tgcgacatcg ggttgatcgc cagcaggcgg
    15781 cgtgctgccg ccgccggtga acccgatgca ggtgtgacgc gtctcttcgg tcaccgccac
    15841 atgttcgggc gcggaggcga cagccaccag ccccgacgca tagtgagtgg tgccgtatcc
    15901 gggatccggc gtgccgatcg ccgcaggcgt gccgcggatc tcgaggtcaa ggcggtcggt
    15961 gatgtggaag aggtgctccg gctcctccaa aggccagcgg ccggtgacgc cggcgagcgt
    16021 ttccgcgaag agcgcgtaac ggatggccgt gcccgcaggg tgggccggga tcgtggtttc
    16081 gtaccggttg ccggaaacgt ttgcgagaaa ttgtgtgtgg accgtctccg gatcggaacc
    16141 ggtacgccag agtaaacgca cggaagcggg atcgtagagg cctatcggtt cgagggaaca
    16201 ggcgacacgg tgggcggtct gggtctcata ggtgttgatc agctccggat gatcgaaaac
    16261 cggcggcgtg ggattgaccc gggccgcgaa gggcgtggcc ggatcgccga acagggtgag
    16321 ttcataaaaa acccaacgcc agtaggtgcc gtaactcggg gagaccaagt gcaggttcat
    16381 gcggcgcgcg cattcgccga tctcgccgag gcgcgtaccg ttgccacgca tcgccgcatc
    16441 ccagaaggag cggtggaagc ggtgcgaaaa cccgccgaca acatggttgt agacccaccc
    16501 ttcccgtgcg ttcatgactg cggcgaacgc ggcattgctg acggtgacca gttgttccgc
    16561 gaagcaatcc ggcgtgtcga acgcgccggc cagacagctc tgcgaataca cgaaatacgg
    16621 cagagtgttg gtgcaggcct gcagtgcggc ctgatgcgcg gaggtgtaca ggttgatctt
    16681 catgcattgt ttcgcgacgg cgtgtccgat atggttgaga atcccgaaat tccggttcag
    16741 gaaagcgaac gcatgctccg tggaccagag tccggcatcc gagtcgtaga gcgtgtgccc
    16801 ggtgtccagc gccggcgcga atgccgaggt ctcgaacccg attgtggtaa gcccgtcgta
    16861 cgtggagccg aaacgcaatt cttccatgaa gccgccggtg taagtcatca gcccgagatc
    16921 catcttttcg gcgaggaatg cgcagggtgc gagttcttcc gctgaagcgg cctcataccg
    16981 cagcgttttg cggatcatgc gggagagttc ttcgggattg gccacgggaa agcggccgac
    17041 atatacctca gccagcaaat cgatatcccc gccgttggca ccgtcctgaa gttcgccgaa
    17101 caggccgttg ccgttcccgt caaacggccc gtcgagacaa ccgtagtata aggcatcggc
    17161 cgggatgtcg gcgatgcgcg tcacgatgat ctcgttgacc gcgacccgga gtttacggac
    17221 cgggatgagg tcgaacgtgc cgccgatcag cagaaaccgc gtgccccagt tccggtgcgc
    17281 gtcttggaca aaggcgcgga tgcggtcggc ccggttttca cccgcatacc gggagtcgat
    17341 ccattccgcg ctgacgcagg ttgtggtgaa tccggtgcgg gcgcgcgccg cgcacagtgc
    17401 cgcgaaattc cagggcgccg gggtgttgtg gatcaggttg gaggtggaga cgatcacata
    17461 cgtgaacggt ccgggcatct cattcagttc ggaggtggca gtggccgcgc ttcgcaccgc
    17521 gataccggtc gggcggaggg cgcggaactc tactgtgacg gtgacggagg ccgcggcgag
    17581 cagcagactg cgttgcggat cgtagcgcac ggggcagact cggacggaaa gccgcgcggc
    17641 gtcggcaccg ttcgggtcca cgcgccagac ggtttgctcg gggtcgggat acgggtgcgt
    17701 gccgccgtag acggccggat cgggcggggc ctccggaaac ggcatctctc cgatgcgggg
    17761 cggcggctgc ccccattcca cgggcgcgtc gagaggaatt tccgtgaccg cgtcgtggag
    17821 gacctgaacc cgatcaactg ccatgcccgg cggaaccgaa aacgtcactc ccgccaccgg
    17881 cagcacgggc aggccggtga cggcatccga ttcacacccc gcgagcccga cgcgcgtgcg
    17941 gccgtccgcc gtgatgcaga ctcgggggag ggcgaacgtc agcgtctgcg tggcgcacgc
    18001 ctccccggga tccgcggcct ggacccgggc accgagcgcg aacacgcatg ccgcaacatg
    18061 ccagacgata ttccgggcta ctgtcatatt cgcagtgtaa gcgaaccgca gaagggcgac
    18121 aacaacgaaa ttcggcttgg tattgatggg cggattcaat acacttacgc gtaattccgg
    18181 taaagttcgg agagtgcggc ggaatggcag aaaggatgca tatgacacga cagcttgcgg
    18241 tgatgttggc tgtggcatgc ttggccggtt cgggccgttc gggggattgg tcccaatggc
    18301 gcggcagcaa tcgcgacttg atcgtcacgg aggagcggct gttgcccgag tggcctgtcg
    18361 gcggtccgca gcggttgtgg catgtggagt tgcccggaga agggtactcc gaaccgattg
    18421 tggtcggcgg cacgctctat atcaccggta gctccggcga gaagaaaagc cgcgtggggc
    18481 acctgtttgc gcttgacccg aaaagcggcg cgatccgctg gcaggtggag tacggaccgg
    18541 agtggggtgc ttcgtatgag ttcgcgcgta cctcgccgac gtttcacgaa ggacgtctct
    18601 acttggttgg cggcctcggg catgtggtgt gcatcgatgc gaaaaaaggc gatatcgtgt
    18661 ggcgtgtcga cagccatgcg gagttcagcg gacgcaacat cacgtggggc attgccgaca
    18721 acccgttgat ttatgacggt aaggtgatct gtcagccggg tggcccggat gccgcgatcg
    18781 tggcgctgga catcaaaacc ggtcgggcgg tatggaagag caaggggctg agcgagcgta
    18841 gcgcctactg ttcgcctgcc ctgctcacga tcaacgggcg gcgtcaggtg gtcaccatgc
    18901 tcgaagacca tgtggtgggc gtggatgctg cgaccggtca gccgctgtgg cgttacccgc
    18961 accgtaacaa atatgcggtt caccccaata cccctgtgtt gtgtggcaag gatcgcgttt
    19021 tcatctcttc gggatacgtc ctcggctcgg cgatccttga gatcgcgggg ggtacggtca
    19081 agcagatttg ggccgacaaa tcctctgaca accactttca gggcgtggcg ttctacaagg
    19141 ggcgcatctt ctcctcgggc ggagggcggc tgacctgctt cgatccggcg gatggccgcg
    19201 cggtgtacca tgtggagggc gcgaaaaaaa catctttttg catcacgccc gtcggcatga
    19261 tcacgtatga cgagcgcggc ggaaccgtga tgttggtgga ggtcggggct gacagccatc
    19321 gggtgatctc ctcgttcaag atcgagtatg gcaacgggcc gcattggtcc tcgccggtgg
    19381 tgtcggatgg cgtcctgtac ctgcggcgcg gtaaggggct tgccgcgttc gcggtgggcg
    19441 actaagagac gccatagcct tagcgtgatc tattttaaca ccgacacccc ctaaatttaa
    19501 taccgacgct tttttgcgtc caaattacct gtcccttagt tgtccgacat cgtgtagcac
    19561 gattggcagg cactttgcaa agtgaaggaa atctttgtcc
//
LOCUS       DAOSRB010000027        19447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2975_length_19447_cov_7.754950, whole genome shotgun sequence.
ACCESSION   DAOSRB010000027 DAOSRB010000000
VERSION     DAOSRB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19447
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2975_length_19447_cov_7.754950"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..2343
                     /locus_tag="PLZ74_02700"
     CDS             133..2343
                     /locus_tag="PLZ74_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOR97261.1"
                     /translation="MDNRKRSSGTKQGYSFGTFGGVFTPSVLTIIGVIMFLRFNVVVG
                     YAGLWNALLILAASKAITVITALSLSSISTNMRVKGGGAYYLISRSLGPEFGGVIAVF
                     FYIAQTVAVTMYIIGFTEAVFSAFPNIPLSFRDVATLSNFIVFLVVYIGANWTIRVQY
                     WILALMMLSILSFFIGGALGFSPEILQSNLAPEWKDGHSLFTIFALFFPAVTGIMAGV
                     NMSGDLKDPSRSIPRGTFAAIGATGLIYALVTFLLAGSVPRAELISDGFVMKDRALFS
                     PLIYAGVFCATLSSALGSMMGAPRILQAFARDDVFRGIKWLGRGSGKNNEPRFAIALT
                     FVIAQAGIMVGDLDTVAPVITMFFLMTYGTVNLACFYEFISRNPSFRPTFRINHWIIA
                     LAGAVGCLVVMFLINAFWAFIAILLALLLYSVIRRIEIMVKWGDMQSGLAYQRARNAL
                     LRLEQERYHPKNWRPTILALSGGAYNRIHLAEYACWLSASHGIVSIGQVIRGNLDNLV
                     ARQREAEFLLRKFIREEGLNAFPAVVVDKNIHAGVQGMLQCHGLGGLRPNTLLLGWSQ
                     DAEKSEVFFGLLTLAKRMERSIVIVANKALDKEAHTVIADGAINIWWNNERDASLLLL
                     LAYLLKKNSEWRTRPIRILRPVPYKADVMSISAAMREMLEGARIEAEIVILPCANPTF
                     AVRTAMQPSAVLFASFDPPDEDNEAATAARMKKIVALPGDVLLVYNAGYNSLQA"
     gene            2412..3893
                     /locus_tag="PLZ74_02705"
     CDS             2412..3893
                     /locus_tag="PLZ74_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOR97262.1"
                     /translation="MSEHNHRDILLVAVNARYSHCSFAARSLLANLGTLERQAAILEC
                     DLEITPVQLAERITAHNPRIVSFSVYLWNVRLIESTARILRLVAPDLKLVAGGSEITS
                     EYPHVNLFDAVIIGEGEAAFRAFCETSIPSPAPSLSTSPSQRDPNSQLLTPNSQFLIP
                     NSLTLPYHLYTDEDLSRRTIYVETSRGCPYNCGYCASAGSGLRLLPLDRILPAFDRLW
                     QRGLRRFKFLDRNFNAPVAHACAVLDFFQERVTPKDTCLHLEINPERLHPDVADRLAR
                     FPAGTLHLEAGIQTLNPQVAAAAGRSPDTATTLANLRFLTRETGADVHADLIFGLPGE
                     DEASFANGFNTLVTTCAPPEVQVNLLKGLPGTRFAREADSLGLVFSPEPPYGLLRSDT
                     MDFETLCRIQRLARCWERVHNRGRFPHAVRAFHAACGPDLFGAYTRLAARLYAGEGRL
                     FAIGMPRLAGYLREELTETCGLGAAEADRLLTADLNAKTLHEG"
     gene            3883..4614
                     /gene="ubiE"
                     /locus_tag="PLZ74_02710"
     CDS             3883..4614
                     /gene="ubiE"
                     /locus_tag="PLZ74_02710"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOR97263.1"
                     /translation="MKVEHITPYGAGEGKREQITRAFDAIAPGYDRLNRLLSLGLDRF
                     WRWRALRLLRAAPSARVLDVATGTADFAIAVARRLPQTRVTGIDLSEAMLDLGREKVR
                     RAGLGARVSLHRGDTLALDFPDASFDTVTAAFGVRNFASLADGLREMRRVLKPGGQVL
                     LLELSRPERFPLRLLHDVYLRVAVPVMGRCLGGHAREYAYLPASIRQVPQGGAMLDLL
                     REAGFRETRCERYTGGICSAYCGVR"
     gene            4812..7295
                     /gene="gyrB"
                     /locus_tag="PLZ74_02715"
     CDS             4812..7295
                     /gene="gyrB"
                     /locus_tag="PLZ74_02715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOR97264.1"
                     /translation="MSDDTRSSSQLDTAQAETHTEKYTAANITVLEGMKAVRKRPAMY
                     VGDTGERGYHHLVYEVVDNSIDEALAGYCTHIDVTINPDGSLSVVDNGRGIPVDLHPV
                     EKRPAVEVVLTTLHAGGKFDGSNYKVSGGLHGVGVSCVNALSEWMEVEVRRNNRIYRQ
                     RFERGHPVTKLEVVGETHGTGTKVTFYPDHTIFTCHEFKWDILANRLRELAFLNRGVS
                     IRFRDMEHEQERDESFLFNGGIVEYVQFLNRNKTPVHDEVIYFSGTRDRVECEIAMQY
                     TDSYNEIEYSYCNNINTIEGGTHLSGFRSALTRTVNKYIADNNLQKSNEEALTGDDIR
                     EGITVVVSVKVPQPQFEGQTKTKLGNGEVEGIVAQIVNDQLGTYFGEKPAVARKVVEK
                     AVLAARARIAARKARDLTRRKGVLDSLALPGKLADCSERDPAKCELYIVEGDSAGGSA
                     KQGRNRETQAILPLRGKVLNVEKARIDKILNNNEIRTLITAIGAGFGADEFDISKVRY
                     HKIIIMTDADVDGAHIRTLLLTFFFRQMPKLIENGYIYLAQPPLYKLTRKQREEYIES
                     DDQLTSKLLLLGADDMTVRTPEGRVLDAKELRRLLELLAQIENTLRAVARRGIDPDTF
                     LSLRKPETGEFPRFATILGSGEDAVFRFVYSDADLANARQEAESKLGAAIDLTETQVQ
                     AYHPTQPMPFRWLEMYRAHLLKKQIDSLVGAGFTVEQVAGGDAPAVFIEEEDESIPVR
                     ALPELLNTVRERGRKGLSIQRYKGLGEMKPEQLYDTTMDPAKRRLLRVRLEDAVKADA
                     IFTLLMGDEVEPRRKFIEDNALNVRNLDV"
     gene            7342..9600
                     /locus_tag="PLZ74_02720"
     CDS             7342..9600
                     /locus_tag="PLZ74_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOR97265.1"
                     /translation="MRLNFTFIGIVALCAAFPAMLTARITDQWRYTLQEPEGAWQAPD
                     FDDTAWKKGSGGFGTAGTPGARVGTQWRTRTIWLRKRFTFARDVAEPALLVHHDEDAQ
                     IFLNGQPVLSLNGFLKEYKVFPLDAAQRALLRPGENLLAVHCRQTTGGQFIDAHVIDA
                     AAVPELPIPERELFSSALITPWGEKVTDQNAWAGYPRPQLRRKAWQNLNGSWEMAVTP
                     VARQEPPLQWQGRIRVPFCLESKLSGVQKMLRQDQALWYRRTFTVAAAERTLLNFEAV
                     DYRCRVFVNGRDVGGHQGGFTPFTLDITDAVRVGENELLVRVEDEQEGFQLRGKQTLM
                     PHGIWYTQVSGIWQTVWLEQVGRTYLADFALRTDAATGTITVTPKVTGTAETLRVEVL
                     ENAKRVAEVAGKAGAPISVRVEKAKCWSPASPHLYDIKLTLLDKDGKVLDQVESYAGI
                     RTVGKTRDAQGHLRFTLNGETIFHWGPLDQGWWPDGLLTPPSEEAMAFEIDWLKRAGF
                     NMIRKHIKVEPRLYYYHCDRIGMMVWQDQVSGGKGAKWTFLKPEPRDAEWPEAHHAQF
                     MLELGRMIDTLENHPCIVTWVPFNEAWGQHRTLDVGAWVARRDPTRLVNIASGGNFWP
                     VGDVVDAHSYPHPAFPFEMGKDGRFDSFIKVVGEFGGHGYLAKGHLWDDERENWGYGG
                     LPKNKAEYKERVAKSITLLNELRQKGIAGGVYTQTTDVEGEVNGLMTYDRKVMKIAAE
                     ELAGLTSVLFAP"
     gene            9661..9745
                     /locus_tag="PLZ74_02725"
     tRNA            9661..9745
                     /locus_tag="PLZ74_02725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9695..9697,aa:Leu,seq:tag)
     gene            9857..10726
                     /gene="hisG"
                     /locus_tag="PLZ74_02730"
     CDS             9857..10726
                     /gene="hisG"
                     /locus_tag="PLZ74_02730"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOR97266.1"
                     /translation="MAVILGLPKGSLQEATFKMMRKAGFVVQAGSRSYSPSVNDPGLQ
                     CRLIRPQEISRYVELGLLDAGITGYDWIYENNSDVHEVSEMCYSKATSQSVRWVLAVP
                     NDSPIQSVKDLQGKRIATEAVGLTRRYLAQHQVEAEVEFSWGATEVKAPELVDAIVEL
                     TETGSSLRANNLRIVDTILTSTTRLIANHKAWKNPAKREKIEQLAVLLQGALAAESRV
                     LIKLNVPADRLDTVTELLPSLHAPTVNRLNKRDWYAVESVVEEQVVRDIVPPLRNAGA
                     TGIIELPLNKVIF"
     gene            10781..10870
                     /locus_tag="PLZ74_02735"
     CDS             10781..10870
                     /locus_tag="PLZ74_02735"
                     /inference="COORDINATES: protein motif:HMM:NF019820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOR97267.1"
                     /translation="MGSIKKKRRAKMSKHKHRKRLKSNRHKNK"
     gene            10987..12495
                     /locus_tag="PLZ74_02740"
     CDS             10987..12495
                     /locus_tag="PLZ74_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97268.1"
                     /translation="MKPIELIALRPLIGLGCAVLGAALLPLLAGCLISGAPHASQTDP
                     STSEEADARRAGALALMAQGLLVESGEGAETNRAKAQAAALTAFRQAVRLDPDGREPL
                     DVLLSALAEREQFAEGFAAAENFLTRHPDDTAIRIEAVRLAEAAARPLEAARHCSLLM
                     EIEPDNRELALARIRLYFMADCENEALEAMRACHARFHDRGSAVLPAHWAVHFLREQE
                     SNPARALRCVDLALAQADEETPVWRGGLHAVAAECHFSLAQTNDALASLHAACREDPG
                     ATGPVLRLGAIWAADPDATNRLAARALREPDTAPTRLILAAVHQEQNNRPAAIATLRE
                     HYTRRLCAGYFPDERFYLWLAGLTEAEKDAAGTERILREALTVHPTSDALQNFLAYLW
                     AEQDIHLDEADRLVTEALQAAPDNAAYLDTKGWILFKKSRPYDALQFLLKAADLDRSE
                     PVILDHTGDVLQAVGRSAEAAAFWTRSHEIDPTPAVTEKLRRHGVAPKPTTP"
     gene            12537..14636
                     /locus_tag="PLZ74_02745"
     CDS             12537..14636
                     /locus_tag="PLZ74_02745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOR97269.1"
                     /translation="MSAFLHIPYLPPFDRLTPEAAASDFPHLLADAHAAVTKVEQALT
                     PSWEGLMRPLYDACRPLFDAWGLLSHMLSVMNSDAWRQVQERLQPEIIAFSQRVAQSP
                     LLYRGYLALREADRASPCLTPVRRRILVKTIQAVEHAGVGLDAERQTRFNAIQNELAQ
                     LATAFRNHVLDATRAFALTLRTPEEAAGLPPALREVTAQAARAAGEPDATAATGPWRI
                     TLDTAVYLPFMMHSRNRTAREQLCRAYATRAASGPTDNTLKIERLLALRRELAGLLGF
                     ESYAELNLADKTARSVAAVDTLIARLAEAAGEAARTESEELLTFARSHGFDEGALQPW
                     DIAFWSERQREHLYDYSDEELSRYFLFPRVLEGLFSLANRLFGIVITPADGEAPVWHP
                     DVRFFRVADADGTPLACFFLDPYSRPETKSGGAWMNDFRTRDRAPDGTVTLPMALLVC
                     NQSVPVGNQPPVMRFQEVGTLFHEFGHALQHMLTTVEEPGASGLNGIEWDAIEIVSQF
                     MENWCYDRATLKGLSRHAETGDPLPDALFDRLIAAKNHRAASAMMRQLFFAATDMDLY
                     ARYPRPEWPDADTVKRLNAARYSPTPLLPEDRFLCSFSHIFAGGYAAGYYSYKWSEVF
                     SADAFAAFEEAGLAQGDAVRAMGRRFRDSLLALGGGTDPAEVFRLFRGRDPSIEPLLR
                     HNGLLRAELRNPAPPAS"
     gene            14633..15595
                     /locus_tag="PLZ74_02750"
     CDS             14633..15595
                     /locus_tag="PLZ74_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOR97270.1"
                     /translation="MNIPPIIRRLLGLVEAAAFLGLLFTLAGLFGRFGWLFEIFTHFK
                     PQFALCFLGYAAFECLARRYRRAAAGLLLAGVNALPVLLLLLPAAPVSLPRSPQPAVR
                     LRLLQANILTHNPDASALLRLVEREQPDVVILQEPNTRWLRDLASLTNRYPVYASMPR
                     EDHFGAAIYCRTNALEAGIFQLDDPEGAPSTCARIRIGDQTLTVIGTHALAPYNAAMW
                     AGRNRFMRMLAQHLRSLGTPLVVTGDFNNTPWSAHFRTFLRESGLIDSAQGRGPLPTW
                     PASLPFLRIPLDHGLHSSDVRIINRRRGPDIGSDHLPLIFDLAF"
     gene            16228..16434
                     /locus_tag="PLZ74_02755"
     CDS             16228..16434
                     /locus_tag="PLZ74_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97271.1"
                     /translation="MGTPNLCVQEIAREVRFDEAQVSLALRALQARGLIAALPFGRQR
                     VSDEITLDLRQCQPYPFHTLKGVK"
     assembly_gap    16514..16613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16614..17040
                     /locus_tag="PLZ74_02760"
     CDS             <16614..17040
                     /locus_tag="PLZ74_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR97272.1"
                     /translation="AQVSLALRALQARGLIAAVRISRWVRYVPEPDPSVPSAKPLLTA
                     LRRALRSGGMSDAEIIRVVTAFTHPRRLAMLRCLHLRAPLSLETLSTACGISMPAANR
                     HFHKLLARGMLVVRSDRISLVSRHPSLTRALLRLAVCGE"
     gene            17237..17872
                     /locus_tag="PLZ74_02765"
     CDS             17237..17872
                     /locus_tag="PLZ74_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97273.1"
                     /translation="MPVKIPPSGQVATPSAGSAAQPARARPRPRPPTVRRNPSRAARK
                     DVAAPSAAGTAAVAEAAAQSSVPAVSSSTVARSAPREQAVKKWEAFVDQITERKTPPE
                     AGQMEQFKNEFMRLHADDQVDAIQNALNLLPDEQFVSLFAILFDKTQNPDVLDEIFND
                     ALNRPEEIKVPMMKALLRDKGHPLHDEAARILDATGEGDADDGNGAEAATP"
     gene            complement(17923..18639)
                     /gene="pyrF"
                     /locus_tag="PLZ74_02770"
     CDS             complement(17923..18639)
                     /gene="pyrF"
                     /locus_tag="PLZ74_02770"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOR97274.1"
                     /translation="MAELIVALDVASTAEVARTVDLLGDAVSFYKIGLELFSAEGPEV
                     VRAVRKRGKRVFLDLKLHDIPRTVERAVRAGAALGVELMTLHAAGGRAMIRAAKEGAR
                     SAGADAPRLLAVTMLTSLDQADLADFGVARSMAEQVEALGRLACESGADGIVCSPQEV
                     ARMRTVLGADALVVTPGVRPAGAEIGDQKRVATPAAAVRNGSTHLVVGRPILEASDPC
                     AAARAIVAEMSAAAYECGGV"
     gene            18816..19244
                     /locus_tag="PLZ74_02775"
     CDS             18816..19244
                     /locus_tag="PLZ74_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97275.1"
                     /translation="MNNTQRIWVWGTVLMAGMLATVPARADRVSVGVGLSTGNGFLTL
                     GINQGGHGHFHRPRAFHPRHPLPRPRPIGVLPPPVVVVPPPVVVAPAPVMVRSGYWQE
                     REERYWVEGCWIETLDTFGRRCKQWQPGHWEVRRIREWVY"
BASE COUNT         3594 a         6088 c         5819 g         3846 t
ORIGIN      
        1 gttttttctc gcacgaaaaa tttaatgaac cgcaacgcct ctccttgacg agaccgtatc
       61 aggtttggca ttgtttacac atgtggacaa tgcaacacga atacgttgcg ttgtaaagac
      121 agagttttgt aaatggataa ccgcaaacgc tcatccggca ccaagcaggg atattcgttc
      181 ggcactttcg gcggtgtctt cacgccgagc gtgctgacca ttatcggcgt gatcatgttc
      241 ctgcgtttca acgtggtggt cggctatgcc ggcctgtgga acgccttgct gatattggcc
      301 gcctcaaagg ccatcacggt catcaccgcg ctttcgcttt cgtccatctc caccaacatg
      361 cgggtcaagg ggggcggcgc ctattacctg atcagccgca gcctcgggcc agagttcggc
      421 ggcgtcatcg cggtcttttt ctatatcgcg cagactgtgg ctgtcaccat gtacatcatc
      481 ggtttcaccg aggccgtctt ttccgctttt cccaatatac cgctgtcttt ccgtgacgtc
      541 gccactttaa gcaacttcat tgtcttcttg gtggtatata tcggtgccaa ttggactatc
      601 cgcgtacagt actggatcct ggccctcatg atgctttcaa tcctttcatt ctttatcggc
      661 ggcgcgctgg ggttttctcc agagattctg cagtccaatc ttgcgcctga atggaaggac
      721 ggacactctt tgttcacgat cttcgccctc tttttccccg ccgtgaccgg catcatggcg
      781 ggtgtcaaca tgtccggcga cctaaaagac cccagccgtt caatcccgcg cggcacattc
      841 gcggccatcg gtgccaccgg tttgatttac gcactggtca ccttcttgct ggccggcagt
      901 gtgcctaggg ctgaattgat cagcgacggc tttgtgatga aagatcgcgc gcttttcagc
      961 ccgctcattt atgcgggtgt cttctgtgcc actctctcat ccgcgctcgg cagtatgatg
     1021 ggtgcgccgc gcatcctgca ggctttcgcc cgcgatgatg ttttccgcgg catcaagtgg
     1081 ctcggccgcg gcagcggtaa aaacaacgaa ccgcgctttg ccatcgcact gactttcgtg
     1141 atcgcgcaag ctggcatcat ggtcggtgac ctcgacaccg tcgcaccggt catcaccatg
     1201 ttcttcctga tgacctacgg tactgtcaac ttggcctgct tttatgagtt catctcgcgc
     1261 aaccccagtt tccggccgac cttccgcatc aaccattgga tcatcgcgct tgccggtgcg
     1321 gtcggatgcc ttgtcgtaat gttcctgatc aacgctttct gggccttcat cgccatcttg
     1381 ttggcgctct tgctgtacag cgtgatccga cgcatcgaga tcatggtcaa atggggcgac
     1441 atgcaaagcg gcctggccta tcagcgtgcc cgcaatgcct tgttgcgcct cgaacaagaa
     1501 cgttatcacc ccaaaaactg gcgccccacc attctcgcgc tttccggcgg ggcctacaac
     1561 cgcatccacc tcgccgagta cgcctgttgg ctcagcgcca gccacggcat tgtttcgatc
     1621 ggccaagtca tacgcggcaa tctggacaat ctcgtcgccc gccagcgcga agccgagttt
     1681 ttgctgcgca aattcatacg cgaagaaggg ctgaacgctt ttcccgctgt ggttgtcgat
     1741 aagaacattc acgccggtgt gcaggggatg ctgcaatgcc acggactggg cggcttgcgc
     1801 cccaacaccc ttttgctggg ctggagccag gacgcggaaa agtcagaggt gttcttcggg
     1861 ctgttaacgc tggccaagcg catggagcgc agtatcgtca ttgtcgccaa caaagcgctc
     1921 gataaggagg ctcacaccgt catcgccgac ggcgcgatta acatctggtg gaacaatgag
     1981 cgcgacgcct ccctcttgct gctgctggcc tatctgctca aaaagaacag cgagtggcgc
     2041 acccgtccca tccgcatcct gcgcccggtc ccgtacaaag ccgatgtcat gagcatctcc
     2101 gcagcgatgc gtgaaatgct cgagggcgcc cgtatcgaag ccgagatcgt cattctgcct
     2161 tgcgcgaacc cgacttttgc ggtgcgtaca gccatgcagc cttccgccgt attgttcgca
     2221 agcttcgacc ctccggatga agacaatgaa gccgcaaccg ccgcgcgcat gaaaaaaatc
     2281 gtcgcgctgc cgggggatgt cctgctggtt tacaacgccg gctataattc ccttcaggcc
     2341 tgaacgggca cacctttcct ttatgtttaa aaacttcgac atgtcgaacg tttgctcaag
     2401 gactgaacct tttgtctgaa cacaaccatc gtgacatcct gctggtggcc gtcaacgcgc
     2461 gttacagcca ctgttctttt gcagcgcgct cgctgctcgc caatctcgga acgctggagc
     2521 gccaagcagc aatactggag tgcgatctgg agatcacgcc ggtgcagctt gccgaacgca
     2581 tcaccgcgca caacccgcgc atcgtcagtt tctccgtcta cctctggaat gtgcgcttaa
     2641 tcgagtcgac cgcgcgcatt ctgcggctgg tagcgccgga tctcaaactc gtggcaggcg
     2701 gctctgagat cacctctgaa tacccccacg tcaatttatt cgacgcggta atcattggcg
     2761 aaggcgaagc cgccttccgc gcattctgcg aaacatccat cccgtccccc gccccctcac
     2821 tctcaacttc cccctctcaa cgagatccca attctcaact cctaactcct aactcccaat
     2881 tcctaattcc caactcctta acccttccct atcacctcta cactgatgaa gatctgagcc
     2941 gccgcacgat ctatgtcgag accagccgcg gttgtccata caactgcgga tattgcgcat
     3001 cggctggcag cggcctgcgg cttctccctc tcgaccgcat cttgcccgcc tttgaccgcc
     3061 tttggcaacg cgggttgcgg cgcttcaaat ttctggaccg caatttcaac gcgcctgtag
     3121 cccacgcctg cgcggtactg gacttttttc aggagcgcgt cacgcctaaa gacacctgtc
     3181 tacacctcga gatcaacccc gaacgcctgc atccggatgt ggccgaccgc ctcgcgcgct
     3241 ttccagcagg cacccttcac ctcgaggcag gcatccagac cctcaatccg caagtcgccg
     3301 ccgccgccgg acgcagcccc gacaccgcca ccacactcgc caacctgcgt ttcctcaccc
     3361 gcgaaaccgg cgctgacgtg cacgccgatc tcattttcgg cctgcccggc gaggacgaag
     3421 cctccttcgc caacggtttc aacacgctgg tgacaacctg cgcaccaccc gaggtgcagg
     3481 tcaacctgct caaaggactc cccggcacac gcttcgcccg cgaagccgac agcctcggcc
     3541 ttgtcttcag tccggagccg ccttacgggc tgttgcgctc ggacaccatg gattttgaga
     3601 ccctatgccg cattcagcgc cttgcccgct gctgggaacg ggtccacaac cgcggccgtt
     3661 tcccgcacgc cgtccgagcg ttccatgcgg cctgcggacc tgatctgttc ggcgcgtaca
     3721 cgcgtctcgc cgcgcgcctc tatgctggcg aaggcaggct tttcgcgatc ggtatgccgc
     3781 gtctggccgg atatctgcgt gaagagctga cggaaacctg cggattgggc gcggcggaag
     3841 ccgatcggct cctcaccgcc gacctcaacg ccaagacact gcatgaaggt tgaacacatc
     3901 accccttatg gcgccggcga gggtaaacgc gagcagatca cgcgcgcgtt tgacgcgatc
     3961 gcgcccggat atgaccggct caaccgtctg ctgtcattgg gcctcgaccg gttttggcgg
     4021 tggcgcgcgc tgcgcctgtt gcgcgccgcg ccgtcggccc gcgtactgga cgtggccacc
     4081 ggcaccgccg acttcgcgat cgcggtggcg cgccgtctgc cgcagacgcg tgtcacgggt
     4141 atcgatttgt ccgaagcgat gctggacctc gggcgggaaa aggtgcggcg cgccggactg
     4201 ggggcgcgtg tgagcctcca ccggggtgac acgctggcac tggatttccc cgacgcctcg
     4261 ttcgatacgg tcacggccgc ctttggcgtg cgcaacttcg cctcgcttgc ggacgggctt
     4321 cgcgagatgc ggcgtgtgct gaagcccggc ggacaggttc tcctcctcga gctctcgcgg
     4381 ccggaacggt tcccgttgcg tcttctgcac gacgtctatc tgcgggtggc ggttccggtc
     4441 atgggacgct gcttgggtgg ccacgcgcgc gaatacgcct acctgcccgc ctccatccgg
     4501 caggtgccgc aaggcggggc gatgctcgat ctgctgcgcg aggccgggtt ccgggagacc
     4561 cgttgcgagc gttacacggg cggcatctgt tccgcatact gcggcgtgcg gtaaaccccg
     4621 gatgacgcac tccacagcga cagccagccg tcttgacgcg ggcggagccc gcaaccctca
     4681 ccccggcctt ctccctgggg gcggggggtg aaaacagctt gtttttaaag aaaaaaaacg
     4741 gggtcatttg cttgtgtcgg acgccttttt aaggtatcct ttattcattc tcaccaacca
     4801 cggaaaaagg tatgtcagac gatacgcgaa gcagttccca actcgacacc gcacaggcag
     4861 aaacgcacac agaaaagtac accgcagcca atatcacggt gctggaagga atgaaggcgg
     4921 tacgcaagcg tcccgccatg tatgtcgggg acaccgggga acgcggctat caccatttgg
     4981 tgtatgaggt ggtggacaat tcgatcgatg aggcgctggc agggtactgc acgcatatcg
     5041 atgtcaccat caacccggac ggttcccttt ccgttgtgga caacgggcgc ggcatcccgg
     5101 tggatttgca cccggttgaa aaacgtccgg ccgtggaggt ggtgctgacc actttgcatg
     5161 cgggcggcaa gttcgacggc tcgaactaca aggtctccgg agggttgcac ggggtgggcg
     5221 tctcttgcgt gaatgccctc agcgagtgga tggaggtcga ggtccgccgc aataaccgga
     5281 tctaccgcca gcgctttgag cgggggcatc cggtcacgaa gcttgaggtg gtgggcgaga
     5341 cgcacggcac cggcaccaag gtgacgttct atccggacca cacgatcttc acctgccacg
     5401 aattcaaatg ggacatcctc gccaaccgcc tgcgggagct ggcctttctc aaccgcgggg
     5461 tctccatccg gttccgcgac atggagcacg agcaggagcg ggacgagtcg ttcctgttca
     5521 acggcgggat cgtggagtac gtccagttcc tgaaccggaa caagacgccg gttcatgacg
     5581 aggtgatcta cttttccggc acgcgcgacc gggtcgaatg cgaaatcgcg atgcagtaca
     5641 ccgattcgta caacgaaatc gaatacagtt actgcaacaa catcaacacc atcgagggcg
     5701 gcacccacct ctccggcttc cgttcggccc tgacccgtac cgtcaacaag tacattgccg
     5761 acaacaacct gcagaagagc aatgaggagg cgctcaccgg cgatgacatc cgcgagggca
     5821 tcacggttgt ggtttccgtc aaggttccgc aaccccagtt cgagggccag accaagacca
     5881 aactcggcaa cggcgaggtc gagggcatcg tggcgcagat cgtcaacgat caactcggca
     5941 cctatttcgg ggagaagccg gctgtggccc gcaaagtggt cgagaaggcc gtgctcgccg
     6001 cacgcgcccg gattgcggcg cgcaaggcgc gggatctcac ccgccgcaag ggcgtgctcg
     6061 acagcctggc cctgcccggc aagctggcag actgttccga gcgcgaccct gccaagtgcg
     6121 agctgtatat cgtggaagga gattcggccg gcggttctgc caaacagggc cgtaaccgcg
     6181 agactcaggc gatcctgccg ttgcgcggca aggttctgaa tgtcgagaag gcccggatcg
     6241 ataagattct caacaacaac gagatccgga ccctgatcac cgccatcggc gcggggttcg
     6301 gtgccgacga gttcgacatc tccaaagtcc gctatcacaa gatcatcatc atgaccgatg
     6361 cggacgtgga cggcgcgcat atccggaccc tgctcctgac cttcttcttc cggcagatgc
     6421 cgaagctgat tgagaacggg tatatctacc tggcccagcc gcctctttac aaactcacgc
     6481 gcaagcagcg cgaggagtac atcgagagcg acgaccaact caccagcaaa ctgctgcttt
     6541 tgggtgcgga cgatatgacg gtccgcactc cggaaggacg ggtccttgac gcgaaggagt
     6601 tgcgccgcct gctcgaactg ctggcccaga tcgaaaacac tctccgggct gtggcccgcc
     6661 gcgggatcga tccggacacg tttctgagcc tgcgcaaacc cgagaccggc gagtttccgc
     6721 ggtttgcgac catcctcggc agtggggagg atgccgtgtt ccgcttcgtg tacagcgatg
     6781 cggatctggc gaatgcgcgg caggaggccg aaagcaaact gggcgcggcg atcgatttga
     6841 cggagacgca ggtgcaggcg taccacccga cacagccgat gccgttccgc tggctcgaga
     6901 tgtaccgggc gcatcttctc aagaagcaga tcgactcgct ggtcggcgcg ggcttcaccg
     6961 tcgaacaggt cgcggggggc gacgctccgg ctgtgtttat tgaggaagag gacgagtcta
     7021 ttccggttcg cgcgcttccc gaactactga acacggtgcg cgagcgcgga cgtaaagggc
     7081 tttcgatcca gcggtacaaa ggtctcggcg aaatgaagcc ggaacaactt tacgatacga
     7141 ccatggaccc ggcgaaacgc cgtctgttgc gcgtccggct ggaggatgcg gtgaaggccg
     7201 atgcgatctt caccctgctg atgggcgacg aggtcgagcc ccgccgcaag ttcatcgagg
     7261 acaacgcgct caacgtgcgc aaccttgatg tctgaccggt ggcgcgggtc ggtctgtacg
     7321 aaaaaggaaa gggagggaaa catgcgtttg aatttcacgt tcattgggat cgttgcgctc
     7381 tgcgcggcgt ttcccgcgat gctgacggcc cggatcacgg atcagtggcg gtatacgctg
     7441 caggagccgg aaggggcatg gcaggcgccg gattttgatg acacggcttg gaaaaaaggc
     7501 tcgggcgggt ttggcacggc cgggacgccg ggtgcgcgtg tggggaccca gtggcggacc
     7561 cggacgatct ggctgcgcaa gcgtttcacg ttcgcgcgcg atgtggcgga gcccgcgctc
     7621 ctggtccatc atgacgagga tgcgcagatt tttctcaacg gacagcccgt gctgtcgctc
     7681 aacggattcc tcaaggagta caaggtcttt cctcttgatg cggcgcagcg tgccctgctc
     7741 aggccggggg agaacctgct ggccgtgcat tgccgccaaa cgaccggcgg acagttcatt
     7801 gacgcgcatg tgatcgatgc cgccgccgtg cctgaactgc cgattccgga acgcgaactg
     7861 ttctcgagtg cgctgatcac gccttggggc gagaaggtga cggatcaaaa cgcgtgggcg
     7921 ggttatcccc ggccgcaact gcggcggaaa gcgtggcaga acctgaacgg ttcctgggaa
     7981 atggccgtga cgccggtggc gcggcaagag ccgccgctcc agtggcaggg ccgcatccgg
     8041 gtgccgttct gtctggaatc gaaactcagt ggcgtgcaga agatgttgcg gcaggatcag
     8101 gcgctctggt accggcgcac gtttaccgtg gcggcagccg aacgcacgct gctgaacttc
     8161 gaagccgtgg attaccgctg tcgggtcttt gtgaatggcc gggacgtggg cggacatcag
     8221 ggcggattca cgccgttcac gctggacatc acggatgcgg tgcgcgttgg ggagaacgaa
     8281 ttgctggtgc gcgtggagga cgagcaagag gggttccaac tgcgcggcaa acaaaccctg
     8341 atgccgcatg gcatctggta tacgcaagtc tccggcatct ggcagaccgt gtggctggag
     8401 caggtcggcc gcacgtatct cgccgacttc gcgctcagaa ccgatgccgc gacaggcacg
     8461 atcactgtga cgcccaaggt taccggcacg gcagagacac tgcgtgtcga ggtgctggag
     8521 aacgcgaagc gggtggccga ggttgccggt aaggcgggcg cgccgatctc ggtccgggtt
     8581 gaaaaggcca aatgctggtc gcctgcctcg ccgcatcttt atgacatcaa gctgaccctg
     8641 ctggacaagg acgggaaggt tttggatcag gtcgagtcgt acgccgggat ccgcacggtg
     8701 ggcaagaccc gcgacgccca agggcacctg cgcttcactc tgaacggtga gacgattttc
     8761 cactgggggc cgctggatca ggggtggtgg ccggacgggt tgctgacgcc gccttccgaa
     8821 gaggcgatgg ccttcgagat cgactggctg aagcgtgccg gattcaacat gatccgcaaa
     8881 cacatcaagg tcgagccgcg cctctattat taccattgcg accgcatcgg catgatggtg
     8941 tggcaggatc aggtgagcgg cggaaaaggc gcaaagtgga cttttctcaa acccgaaccc
     9001 cgggacgcag agtggccgga agcacatcat gcgcagttca tgctggagtt agggcggatg
     9061 atcgacacgt tggaaaatca cccctgcatc gtcacttggg tccccttcaa cgaggcgtgg
     9121 ggtcagcacc gaacgcttga tgtcggggcg tgggtcgcgc ggcgcgatcc gacgcgtctc
     9181 gtgaatatcg ccagcggcgg gaacttctgg ccggtggggg acgttgtgga cgcccacagc
     9241 tatccgcacc ccgcttttcc gttcgagatg ggaaaagacg gtcgttttga ttccttcatt
     9301 aaggtggtcg gggagttcgg cggacatgga tatctggcga aaggacattt gtgggatgac
     9361 gaacgcgaga actggggcta cggcggtttg ccgaagaaca aagccgagta caaagagcgc
     9421 gtcgcgaaat ccatcacact gctgaacgag ctgcgacaaa aaggcatcgc gggcggcgtc
     9481 tacacgcaga ccacggatgt cgaaggggag gtcaacggtt tgatgaccta tgaccgcaag
     9541 gtcatgaaga tcgcggcgga agagttggcc gggctgacca gtgtgttgtt cgcaccgtga
     9601 cgcggtttcc ggcaatgcgg catctggact gctgacgggg tgcgttgcgg tcctctgtgc
     9661 gggcgagtgg tggaattggc agacacgcaa gacttaggat cttgtgccgt aaggtatgcg
     9721 ggttcgagtc ccgcctcgcc caccaccgaa cgatcggcgg ttcaaaaatc gatttcgcgg
     9781 tacttgccta ctttaggtta atccgataag atgtttaacc ttttatcaaa ccaatcggaa
     9841 agagaccaaa ggcgttatgg ctgttatttt gggattgccc aagggaagtt tgcaggaagc
     9901 aactttcaaa atgatgcgaa aggccggatt tgtcgttcag gccggatcgc ggtcttattc
     9961 acccagcgtg aacgaccccg ggttgcagtg tcggctgatc cgccctcagg agatttcgcg
    10021 ctacgtggaa ctcgggcttc tggatgcggg catcaccgga tatgattgga tttacgaaaa
    10081 caactccgat gtgcacgagg tttcggagat gtgctactcc aaggccacct cgcagtccgt
    10141 gcgctgggtt ctggccgtgc ccaacgactc gccgatccag agtgtcaaag acttgcaggg
    10201 caaacgcatc gccaccgagg cggtcggtct gacgcggcgg tacctcgccc agcatcaggt
    10261 cgaggccgag gttgagttct catggggtgc caccgaggtc aaggcgccgg aactggtgga
    10321 cgccatcgtc gagctgacgg agacaggctc ctcactgcgt gccaacaacc tgcggattgt
    10381 ggacaccatt ctcacctcga ccacccggct gatcgccaac cacaaggcgt ggaaaaatcc
    10441 ggccaagcgc gaaaagatcg aacaactcgc cgtgctgttg cagggtgcgc tggcggctga
    10501 gtcgcgtgtg ctgatcaagt tgaatgtgcc ggccgaccgg cttgacaccg tgaccgaact
    10561 gctgccgtcg ctgcatgccc ctacggtcaa ccggctcaac aagcgtgatt ggtatgcggt
    10621 cgagtcggtg gtcgaggaac aggtcgtgcg cgacatcgtc ccgccgttgc ggaacgctgg
    10681 cgcgaccggg atcattgagc tgccgctgaa caaagtcatt ttttgaacaa aacaacagga
    10741 caccccatcg ggtacgccct gcacttccag gagaaagaac gtgggttcga tcaagaaaaa
    10801 gcgccgtgcg aagatgtcga agcataagca ccgtaaacgc ctgaagagca atcgccacaa
    10861 gaacaagtaa ggcgccgagg cgcacaccgg cgcgtgaccg cctgcggatg ggatagccgt
    10921 gccctttctc cccgtttccc ggaaaggaaa ggggtgtggc gtgtgatgtg tattggagac
    10981 cccgacatga aaccgattga gcttattgcc ctgcgtccgc tgatcggttt ggggtgtgcg
    11041 gttctcggtg cggcgttgtt gccgttgctg gccggttgtc tgataagcgg cgcgccgcac
    11101 gcctcgcaaa cagacccctc cacttctgaa gaggccgacg cccgtagggc tggcgctttg
    11161 gcgttgatgg cccaagggct gcttgtcgag agcggcgaag gtgccgaaac caatcgcgcc
    11221 aaggctcagg ctgcggccct gaccgctttc cgtcaggcgg tccggctcga ccccgacggc
    11281 cgcgaaccgc tggacgtcct gctgagcgcc ctcgccgaac gcgaacaatt tgccgaggga
    11341 ttcgctgcgg ctgagaattt cctcacgcgt catccggacg acacggctat ccgtattgag
    11401 gcggtgcgtc tggctgaggc ggccgcccgc ccgttggagg cggcccgcca ctgctcgctt
    11461 ctgatggaga ttgagccgga caaccgggag ttggctctgg cgcgcatccg actctatttc
    11521 atggccgatt gcgagaatga agcgctcgaa gccatgcgcg cctgtcacgc gcgttttcac
    11581 gatcgcggca gtgcggtgct tccggcacac tgggcggttc actttctgcg ggaacaggag
    11641 agcaaccccg cacgcgcgct ccgttgcgtc gatctcgccc tcgcgcaagc ggacgaggag
    11701 acccccgtgt ggcgcggcgg cctgcacgct gtcgcggcag aatgccattt cagcctcgct
    11761 caaaccaatg acgccctcgc cagcctgcac gccgcctgcc gggaggatcc cggcgccacc
    11821 ggtccggtgc tgcgcctcgg cgcgatctgg gctgcggatc cggatgcgac caatcgtctg
    11881 gccgcacgcg cgctccggga acccgacacc gctcccacac gcctgatcct cgccgcagtt
    11941 catcaggaac agaacaaccg gccggcggcc atcgccaccc tgcgcgaaca ctacacccgt
    12001 cgtttgtgcg ccggatactt tccggacgag cgtttttatc tctggctcgc cggattgacc
    12061 gaggcggaaa aggatgcggc ggggacggaa cggatcctgc gtgaggcgct caccgtgcac
    12121 cccacttcgg acgcgctcca gaattttctg gcctacctgt gggccgagca ggacatccat
    12181 ctggatgaag ccgaccggct ggtgaccgaa gccctgcagg ccgcacccga caacgccgcc
    12241 tatctggaca ccaaaggctg gattcttttt aagaaaagcc gcccctatga cgcgctccag
    12301 tttctgctga aggccgcaga cctcgaccgc agcgaaccgg tcattctcga ccacaccggc
    12361 gatgtccttc aggcggtcgg acgttcggcg gaagccgctg ctttctggac gcgcagtcat
    12421 gagatcgatc cgacgcccgc cgtgaccgag aaactgcggc ggcacggggt cgcgccgaaa
    12481 ccgaccactc cttaacccct gcggaaccgg gcccctttcg cacgcacacg cttgttatgt
    12541 ccgctttcct ccatattccc tatctgccac ccttcgaccg cctgacccca gaggccgccg
    12601 cttctgattt cccgcatctg ttggccgacg ctcatgccgc agtcacaaag gtggagcagg
    12661 cgctcacccc gtcgtgggaa ggactgatgc ggcctttata tgacgcctgc cgcccgcttt
    12721 tcgacgcatg ggggttgctt agccacatgc tttctgtgat gaacagcgac gcctggcgtc
    12781 aggttcaaga acgccttcag cccgagatca tcgccttttc gcagcgcgtc gcacagagtc
    12841 cgctgctgta tcggggatat cttgccctcc gcgaggccga ccgcgcctct ccctgcctca
    12901 cgccagtccg gcggcggatt cttgtcaaaa ccattcaggc cgtcgaacac gctggcgtgg
    12961 ggcttgacgc ggaacggcag acacggttca acgccatcca gaacgagttg gcgcaactcg
    13021 ccaccgcctt tcgcaaccat gttcttgacg ccacccgcgc gttcgctctc acgctgcgca
    13081 caccggagga ggccgctggc ctccctcccg cactccggga ggtcacggcc caggccgcgc
    13141 gcgcggcggg tgaaccagac gccaccgccg ccaccggccc gtggcgcatc accctcgaca
    13201 ccgctgtata cctgccgttc atgatgcata gccgtaaccg cacggcacgc gaacaactct
    13261 gccgcgccta tgccacccgc gccgcttccg gccccacgga taacacgctg aagatcgagc
    13321 gtcttcttgc cctgcggcgc gaattggccg gtctgctcgg attcgaatct tacgccgagt
    13381 tgaacctcgc cgataaaacc gcgcgctcgg tcgctgcggt cgatacgctc atcgcccgcc
    13441 tcgcggaagc cgccggtgaa gcggcccgca ccgagtcgga agaactgcta accttcgccc
    13501 gcagccacgg gttcgacgag ggcgcgctgc aaccgtggga catcgccttc tggtccgagc
    13561 gccagcgcga acacctctat gattacagcg acgaggaact gagccgttac tttctcttcc
    13621 cgcgtgttct ggaagggctc ttcagccttg cgaaccggct cttcggcatc gtcatcacgc
    13681 ccgccgacgg cgaggcgccg gtgtggcatc ctgacgtgcg ttttttccgg gtcgccgatg
    13741 cggacgggac tccgctcgcc tgtttctttc tggaccccta cagccgtccg gaaaccaaaa
    13801 gcggcggcgc gtggatgaac gatttccgga cgcgggaccg cgcgccggat ggtaccgtca
    13861 ccctgcccat ggccctgctg gtctgcaacc agagtgtgcc ggtggggaat cagccgccgg
    13921 tcatgcgttt tcaggaggtc ggcacgctct tccacgaatt cgggcatgcg ttgcagcaca
    13981 tgctcaccac cgtggaagaa ccgggtgcct cgggactgaa cggcatcgag tgggacgcga
    14041 tcgagatcgt cagccagttc atggagaact ggtgttacga ccgtgccacc ctgaaggggt
    14101 tgagtcgcca tgccgagacc ggggaccctt tgcccgacgc gctgtttgac cggctgatcg
    14161 cggcgaaaaa ccaccgcgct gccagcgcga tgatgcgcca gctctttttc gctgccacag
    14221 acatggatct gtacgcgcga tatccgcgtc cggagtggcc tgacgctgac accgtcaaac
    14281 gcctgaatgc cgcgcgctac tctccgaccc cgctgctgcc ggaagaccgg ttcctctgtt
    14341 ccttcagcca tattttcgcg ggcgggtatg cggccggata ctacagttac aaatggtccg
    14401 aggtcttttc cgccgacgcc ttcgccgctt ttgaggaggc gggactcgcg cagggcgatg
    14461 cggtccgcgc catggggcgg cgcttccgcg actctctgct ggcgctgggc ggcgggaccg
    14521 atccggccga agttttccgc ctcttccgcg gccgggatcc gtcgatcgag ccccttctgc
    14581 gccacaacgg cctgctccgg gctgaattgc gaaaccccgc tccgcccgct tcatgaacat
    14641 cccgccgatc atccgacgcc tgctcggcct tgtcgaagcc gccgcctttc tcggtctgct
    14701 tttcactctg gcgggactct ttggccgctt cggatggctt ttcgagatct tcactcactt
    14761 caaaccgcag tttgctctct gctttctcgg atatgcggca ttcgagtgcc tcgcgcgccg
    14821 ctaccggcgt gcggcggctg gtcttctgct agctggcgtc aacgccctgc ctgtgctact
    14881 gcttctgttg cctgcggcac ccgtttccct accccgctcc ccccaaccgg ccgtgaggct
    14941 ccggttgctt caggccaata tcctcactca caaccccgac gcctcggctc tgttgcgcct
    15001 cgttgaacgg gaacagcccg atgtggtgat tctgcaggaa cccaacaccc gctggctccg
    15061 cgatctcgcg tcgctcacca accgttatcc ggtttacgcg tccatgccgc gcgaggatca
    15121 tttcggcgcg gcgatctact gtcggacgaa cgcgcttgaa gccggaatct tccagctgga
    15181 tgacccggag ggcgcaccct ccacctgcgc acgtatccgt atcggtgacc agaccctcac
    15241 cgtaatcggc acgcacgcgc tcgccccgta caacgccgcc atgtgggcgg gacgtaaccg
    15301 tttcatgcgg atgcttgcgc aacacctccg gagtttggga actccgctgg tcgtgaccgg
    15361 tgacttcaac aacactccct ggtccgcaca cttccgaacc ttcctgcgtg agagcggtct
    15421 gattgacagc gcccaaggcc gcggtccgct ccccacttgg cccgcctctc tgcctttcct
    15481 ccgcattccg ctggatcacg gcctgcattc atccgatgtg cggatcataa atcggcgccg
    15541 cggcccggac atcgggtcgg accatctgcc cctgatcttt gatctggcct tttgaggctg
    15601 cggaaagctt gtcagcagga ggcggtcgag aaaatcattg cgggtttcgg ttttgcagcc
    15661 gtgccgctgg cgcacaccag ttggagttac ggacgccatt tggtgccttg tcggccgata
    15721 aggccaataa aaaacaagtt gtttctcaca cgaagcgaag gatgcgaaga gcacgaaggg
    15781 gaatggggtg ttatggaaac ccctttgtgt ctgttgagcc tttgcgtgag gctaaattgg
    15841 gttgcccgta ttaggagttt ggcctttcta gtcgttcgga ttttgcgggc tctgcccgta
    15901 ttaggagttt ggcctttcca gtcgcgcgga ttttgcgggc tctgtccgta ttaggagttt
    15961 ggcctttcca gtcgcgcgga ttttgcgggc tctgcccgca tcaggattgg gggcgcccgt
    16021 tgaaagtgga catgaaaaac cattttacca cttggacgac aaagggttct cagacgagaa
    16081 cacacttgat ttgcgtcaat gccagccgta tccttttcac acctttcaag gtgtgaaatg
    16141 acgccggaca gaacctatca tccagcgttg tggcggactt gccgggtttt ggcgaacgca
    16201 aggcggttgg cctgccttcg ggtggtcatg ggcacgccga atttgtgcgt gcaggaaatc
    16261 gcgcgcgaag tgcggttcga cgaagcgcag gtcagtctgg cgcttcgggc gttgcaggcg
    16321 cgtggtctga tcgccgcttt accatttgga cgacaaaggg tctcagacga gatcacactt
    16381 gatttgcgtc aatgccagcc gtatcctttt cacaccttga aaggtgtgaa atgacgctgg
    16441 acagaaccta tcatcctacg ttgtggcgga cttgccgggt tttggcgaac gcaaggcggt
    16501 tggcctgcct tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagcgcag
    16621 gtcagtctgg cgcttcgggc gttgcaggcg cgtggtctga tcgccgctgt gcgcatatcg
    16681 cgttgggtgc gttatgtgcc ggagcccgac ccgtccgtcc cttccgcgaa accgctgctg
    16741 accgctctcc gccgtgcgtt gcgttctggc ggaatgtccg atgcggagat tatccgggtg
    16801 gtcaccgcgt tcacccatcc gcgacgtctg gccatgttgc gttgcctgca tcttcgtgcg
    16861 ccgctttccc ttgaaacgct gtcgacggcg tgcgggatca gtatgcctgc cgccaaccgt
    16921 cattttcata agctgctggc tcgcgggatg ctcgtcgtcc gcagtgatcg catttcgctt
    16981 gtgtcgcgcc acccctcgtt aacccgcgct ctgctccgtc tcgccgtatg cggtgaataa
    17041 cggacgttgc ggaaacgcgg cggtcttttg aaactgcgga taaacccgct tgtcaccaca
    17101 caccggatca gagtaccata cggatatgac tcgatggacg caaacgaggc tcgtctttct
    17161 agcggttagc acgattttgg tcgcctctct ttgttggcgc aaccgcacgg tgcgtcaaga
    17221 cggtggtgtt tcggacatgc ctgtcaaaat tccgccatcc ggtcaggttg cgactccctc
    17281 cgccggttcc gccgcgcaac cggcacgcgc tcgcccgcgc ccgcgcccgc ccactgtccg
    17341 ccgcaatccg tcgcgtgccg caaggaaaga tgttgcggca ccgtccgccg caggcaccgc
    17401 tgcggttgcg gaggccgcgg cgcagtcgag cgtgcctgcg gtctcaagtt cgaccgtcgc
    17461 gcgctctgct ccccgcgaac aggcagtcaa gaagtgggag gcgttcgtgg accaaatcac
    17521 ggagcgcaag accccgccgg aggccggcca gatggagcaa ttcaaaaacg agttcatgcg
    17581 cctgcatgcg gacgatcagg tggacgccat ccaaaatgcc ctcaacctgc tgccggatga
    17641 acagttcgtc tccctgttcg ccattctttt cgataaaacc caaaaccccg atgtgctgga
    17701 cgagatcttt aacgacgccc tgaatcggcc cgaagagatc aaggtgccga tgatgaaggc
    17761 tctgcttcgg gacaaggggc atccgctcca tgacgaggcc gcgcgcattc tcgatgcgac
    17821 aggcgaaggg gatgccgacg acggaaacgg cgcagaagcc gcgacgcctt aatctgagga
    17881 aggcttcgcg gctcctgttc gggcgcgctg tccagccacc cgctacacgc ccccgcactc
    17941 atacgcggcc gcgctcatct ccgcgacgat cgcccgcgcc gccgcgcacg ggtcggacgc
    18001 ttcaaggatc ggacggccca ccaccagatg cgtcgaaccg tttcgcactg ccgcggcagg
    18061 tgtggccacg cgtttttggt cgccgatctc cgctccggcc gggcgcacac cgggcgtgac
    18121 cacgagcgcg tccgcgccca gcaccgtgcg catgcgcgcc acctcttgag gcgagcagac
    18181 aatgccgtcg gcaccgctct cacaggcgag acgccccagc gcctcgacct gttcggccat
    18241 gctccgcgcc acaccgaaat ccgcgaggtc cgcctgatca agactggtca gcatggtgac
    18301 cgccaacagc cgcggcgcat ccgcgcccgc actccgggcg ccctctttcg cggcgcgaat
    18361 catcgcccgc ccgcccgccg catgcagggt catcagttct acgcccaacg ccgcgccggc
    18421 ccgcaccgca cgttcgacgg tgcgcgggat gtcgtgcagt ttgaggtcga gaaaaacccg
    18481 cttgccgcgt ttcctgaccg cccggaccac ctccggcccc tcggcactga acagttccag
    18541 cccgatcttg taaaacgaga cggcatcacc cagcaggtca accgtccggg ccacctccgc
    18601 tgtagaggcg acatccaacg ccacaatcag ttccgccatg tttccgtatc ctttgctttg
    18661 gtcgtattaa gccacaaaag gaccgcgcgg gcaatcccgg agggatctga ctttttttcg
    18721 ctgtgacggc aatacggaag ttaaaccgtc gtttaataca actgtttgag acattcggcg
    18781 gggggaaccc ccgcaggcgt cagaaaggtg gggacatgaa caacacacag cgcatatggg
    18841 tttgggggac ggttctgatg gcgggcatgc tggcgactgt tccggcgcgg gctgaccgcg
    18901 tgagtgtcgg agtgggattg agcaccggca acggcttcct gaccttgggt atcaatcagg
    18961 gcgggcatgg gcatttccac cggccgcgcg ctttccatcc tcgtcaccct ctcccgcgac
    19021 cgcggcccat cggcgttctg ccgccgcccg tggttgtcgt gccgccgccg gtcgtggtgg
    19081 cgcccgctcc ggtgatggtc cgcagcggct attggcagga gcgtgaagaa cgctattggg
    19141 tcgaagggtg ctggatcgag acgctggaca cgttcggacg ccgctgcaaa cagtggcaac
    19201 ccggacactg ggaagtccgg cgcatccgcg aatgggtgta ctgaccggcc aggccgagtt
    19261 cccgatcagg gagttccaag ccttaaaaga acgcctctta cgtcaaacga aacgacaccg
    19321 gattccggtg tcgtcttcgt ttgacttggg aacagatacg tgtatacttt ttaaaattca
    19381 gccgttggat ttttgcgcgc gcgcgaccgg ccagcgcaac acctaaagtt ttgaaagcgg
    19441 ccacgcg
//
LOCUS       DAOSRB010000028        19439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_2979_length_19439_cov_7.925299, whole genome shotgun sequence.
ACCESSION   DAOSRB010000028 DAOSRB010000000
VERSION     DAOSRB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19439
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2979_length_19439_cov_7.925299"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2230)
                     /locus_tag="PLZ74_02780"
     CDS             complement(<1..2230)
                     /locus_tag="PLZ74_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97276.1"
                     /translation="MASRKHRFFTTPWQLIRFFLPVVTLLTAVVYAVTLFRHLYPGTP
                     AFLTAMAAGLCQQNDLAHPLFSLIIRPIAQLPYATLPVRLNLFCAACGTLAATLFYLL
                     TARLIFIWAAENPGGSMAALPPGTEGIREDEGDLPNRNRMSFPLPASEMPILPASVQA
                     HNWRSARAAALGGMGAALAFAFCGPFWIAATRLYPFAFDLALFFLLLNLLIAYDQKAH
                     PFFLFASSFLLAACCVESPLFLLLLPVGSGFLLRSLLLNEHATFYRTLAVVLTGLIGF
                     LTGGMLLWRASALCAAVPIPAPRPILNVLTATMLDELARWVPSFGWSYIFMQLLFPCA
                     IALFIFAFSFRKRTPLLFLMQLTLAATLIPSLLNLRMSPWAIARMTSRIPVFSSTIVA
                     FLTGLLIAVWYLMREMFDEQLSEDLDYYEYRDHPLVCRIGRWLCWPLLALACLVPFRS
                     FTDIDPREGAFVDVVTDRIYATLDTCDWIVNSSTLTHHLMLRAFQDGRRLRLIETEES
                     PDGANAKHLGTAIREDPSLTRIQARLLNAADLSPVSFLREWLTQETNAYSRIALFRTP
                     VFWRENGFTPLPNSIFFNGLPQDVSVDLAQLRDRHQAFVEFLQPHLFPATPDSIRLFA
                     KIRAALAGQLACAGNELAVLFAGQKRFEEAAELLAQTEALMPDNLSVLLNRYHLALGH
                     GLRADTLPALQTRLREIPRRYARTHLLTPASVQAQTGTLISPDILEQVRKSYWTKSGL
                     LQQ"
     gene            complement(2240..4393)
                     /locus_tag="PLZ74_02785"
     CDS             complement(2240..4393)
                     /locus_tag="PLZ74_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97277.1"
                     /translation="MKTLLLFCCLLASGLGAADLYVAPAAKTSEPPDGSRRRPFTSLT
                     QARDAIRAARKAGCSEAWTVHVDTGFYPVNEPIVFSPEDSGTADAPVTYRGRGADRSR
                     LCGGRVITGWQVQPDGRWLADIPCDASGTPAYFETLFVNGRRAPRARHPNRGFFNPAA
                     VQQTILTNASPRGVYARATLTGRAGELDPLARSPQPEWRFAQVVVHHKWDTTRRMIAG
                     FDVGKATLITQGAPWKHWNKWQTNSLYYVENVRSALDAPGEWFYDGVNRKLHYLPLPD
                     ERPERAEVVAPLSGVKTLVVFQGDPVAGVFVRHLTFENLGFLYTDSPRRLNQIACAFL
                     PDEICGPLNAPGPTQFEPHQAAAHTEAAIMADGAHGVTFRRCDIAHTGEYGLWFRAGC
                     VSNRVEQCALTDLGAGGIRIGDPGAKGVSASTNAAVQALTSFSTAFNTVDNCIITHGG
                     RFHASGTAVWIGHSPDNSVTHCEISDHFYTGVSVGWVWGYRGSAAQRNTIAFNRIHTI
                     GQRMLGDMGGVYTLGTSFGTCISNNVIFNVDSYTYGGWGLYPDEGSEGIVLENNLVYD
                     TKDGSFHQHYGRDNVLRNNILAFSRECQVAVTRVEPHRSLTVERNIIIWENAPNFSDT
                     RYTRSIQAKVDWQSNLWWRTDGPADFKGMTFAEWQAMGRDTDGLVADPLFENAAERDF
                     RLRRGSPAEQIGFRPFDFSRAGVYGDRAWRRRAHQ"
     gene            complement(4423..5253)
                     /gene="kduI"
                     /locus_tag="PLZ74_02790"
     CDS             complement(4423..5253)
                     /gene="kduI"
                     /locus_tag="PLZ74_02790"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOR97278.1"
                     /translation="MRNIETPDRQSYRRMTTDELRNRFVIEDLFQPGQVNLVYTNTDR
                     AVVGGIVPETEHLELTGGREMACSYFCERREVGIINLGEPGTVVVDGTEHALARRECL
                     YVRRGSRGLLFSSANPQKPACFYLVSYPAHTDYPTVKAGLKDANRIELGTLAESNRRT
                     IYQYIRPGFIRSCQLVMGFTMLHEGSVWNSFPPHTHDRRTEVYCYFDLPPNGAVLHLL
                     GAPDETRHVMLREKQVVLSPAWSVHCGAGTGGYAFVWAMGGENQEFDDMDKCDLASFR
                     "
     gene            complement(5375..6580)
                     /gene="hydF"
                     /locus_tag="PLZ74_02795"
     CDS             complement(5375..6580)
                     /gene="hydF"
                     /locus_tag="PLZ74_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOR97279.1"
                     /translation="MSLNDIPQGERVHIALFGRRNVGKSSLINALTGQEVSIVSAVKG
                     TTTDPVRKAMELLPLGPILIFDTPGLDDESELGALRIERTRDVLNKTDVAILVLEADS
                     GIGDCEREVLDLVRAKGIPLLTVFNKVDLRPPSPSALRQAAEECKTPLLTVSSVTRAG
                     IEDLKLALAKVAPEDDAERLVGDLLKPNDLVVLVTPIDSAAPKGRLILPQQQTVRDIL
                     EAGALALVTREFELRAALATLARPPRLVITDSQVFESVAAEIPRNVPLTSFSILFSRY
                     KGDLAEQVRGTRAIASLKDGDRVLVAEGCTHHRQCDDIGTVKIPRWLLRHTGRKLVYE
                     HTSGMNYPSDLSPYALIVHCGACMLNRREMRYRIHQAKAAGVPIVNYGVLIAHLHGIL
                     ERVIEPFTQ"
     gene            6797..9046
                     /locus_tag="PLZ74_02800"
     CDS             6797..9046
                     /locus_tag="PLZ74_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOR97280.1"
                     /translation="MRKIRFLVWVMGASLAFGRGESWMPKPPAPPSVATLTNEARYVE
                     WKFRGDVTDPSTGLAAHALRTNALALASGLEPKEDWRVVKAKVYAMLCDQMAIDVSPL
                     DWYPAISIWNRYDRPMSAVVWRRNGEIARKCYPDITGRINKGNRDGIWRVWKDFDHSV
                     PVWEDMFKLGFAGMRDRVDAHDDGTPFYRSLRITADASIRLIERFAAQARKRLGTADG
                     KSSRLVKQIAALDQLAKGPPQTAYEVLLFIDLYFFMCEHLDAMQCRSLSIIDRNLWPY
                     YQADLAAGRTTEAEFREQFRHFLWQWGSVDNYWGQPVTMGGTKADGTTEYNPLSRIIL
                     DVMDECALPTPKFHVKIGKSTPREILDKLLSMARRNRSLTFIGEESARRILEHYGATK
                     EEARTFYTTGCYEIVLANGANKTGVGYINLLKQVEFVLRDAKGGKFSAADYAAFQAEC
                     IRRMVNVCTNCLAITSAFEQHLDDVNPANITTLGVAYAVKTGRDAFANGSERGNNTAI
                     LIAAMGTTVDALVAVKDLVYEKKEMSLADLGKLMAANWEGRDDLRLRMKRSKRKWGNN
                     DPDANATAAAVSKALAAAVNGKPNARGGIYVLSGHSARAFIYEGAATGATPDGRKAGE
                     EFSKNMSATMGADTEGVTALIQTLGALDSRDWPGDFPLDVMLHPATVEGDRGLALMRT
                     LLDVYYANGGLQIQFVVVSADELRDAQKHPEKYENLQIRVCGWNVRWNDIPKAEQDAY
                     ILRAESIMQ"
     gene            9043..9870
                     /locus_tag="PLZ74_02805"
     CDS             9043..9870
                     /locus_tag="PLZ74_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR97281.1"
                     /translation="MNRREFIGSSAAFAVAPLFAAGGGKVRTPLMLDTKRMAIWDGPG
                     VRTTFFVKGCPLKCIWCHNPESISAQRQTARFQHLCRRCEKCTMDEATCPTRALKFYG
                     KPMKIDEIVAKALEDKPFYDSSGGGVTLSGGEPLLFWEWLAELFTAFKKAGLHTCLDT
                     SLYAPPVAIEAMLPLVDMWLPDYKADDDARHRKYTGVPNKIIRKNLERLVAAKAKLEV
                     RLLVVPRCTDDDDLAARHAYLDSLDIPRAAQVELEYRDLARSKYLALGMPDTMPPST"
     gene            10084..12213
                     /locus_tag="PLZ74_02810"
     CDS             10084..12213
                     /locus_tag="PLZ74_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOR97282.1"
                     /translation="MRLHENCRMNNFKLGVVALGGVMAGTILARPVLQPEVKEYVSSK
                     GVADYAAAPVVYQDQRQCRIGADEIPSTGEKTAYAGHALPEAGVFIAIADSALGKKLV
                     ADFRLDVPAKKQGYAIRVDGKCAVIVGHDAIGALYGAVTFRQMAAKGGRVECAMVRDW
                     PDVLYRGGASLGRGLWPYGKSDGVDGIKAALDVMMRHKLNIISDFFGLDELAPREKLA
                     TWRELVRYARDRGIYSYYFISTALFRRTNKPEGMTLEKWPCVKDLRTWNEWYYCWSDD
                     EGTEKAVNHCADILQNIGAEDGIVQIHPVDGGGWKDPEFWSKRCAKCRSLWNDHERWK
                     ATVHQLEIWNRVLRRRCPNVIVGSCIYPYTYSALLTPEKDRTDLWRESHTEYWSKVSE
                     GIGNKEFFFSSWMSPPWLAEQIRRYVPSRPIHFAQYPQFSGIFQTTSRKACSVYEENA
                     DNTYLPQGTDFDAVQWESLLLGVESTWNRNMPGAEAFDGGTYYDAAVDHTGPRIVMEQ
                     VLPRLCAAFWGTELAPYMTRILSSGVLPSYIRDPGKALAYWNSVRKDPLFDPTVEQVS
                     TLERKRIEKIVDTSERMREQVKAAETVIRDLTEARGHLDGLDSVRRRYFMKLAKYAPF
                     WLATARARYALRAANDLIAAGRHEEAKRALPEGLRQLREEWRQAEENLKALSSEVDCS
                     PRRYVWPVKLETLVAEFERAETSAGRP"
     gene            12478..14679
                     /locus_tag="PLZ74_02815"
     CDS             12478..14679
                     /locus_tag="PLZ74_02815"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOR97283.1"
                     /translation="MASRRMFFALIILLSAIPDRTARAVSAEWTLETVDRNARGVADW
                     ARPNPKRSLVWENGTLYAGITALGLADPSLPYLDQIRQLGAHHAWGHRERTYHADDHC
                     IGHAWLELAAFDNNPVYAARLRRNFEYILANRHRGSLNWSEKDCAKRWCWSDALFMAP
                     PVWVKMYGLTGDRRYLDFLDQEYKLTTDLLYDKEASLFYRDSRYLTERTKNGKKVFWG
                     RGNAWVYGGLALMLRDLPADWPARADYTKLFREMSAALKKTQGTDGAWRPSLLDPDDP
                     DLPEMSCTTFFVYGMLWGVNNGVLDKETYLPGIKKAWAVISASINGEGRLGWTQPPGW
                     APEKAFNADTHAVYAIGSYLLAAMELRKQIVADAHPERKTVTATNPSERFRSAETLSL
                     NWGELKLDAAKTRVFDVRNAAFLPHQLIDSDLDGVPDELIFRSYFAARQTREFWVFED
                     SGIPPPVSPEICFSRHVPERMDDFAWENDRMAYRIYGPAVSLPPPQGEGLHSSGIDVW
                     CKKVRHPVINTWYKHRRYHDDHGEGMDFYKVGPGRGCGGLGVFHAGAFHVSGNWATWK
                     HIANGPVRTVFEVTYAPWACGNGAQVAETRRVSLDAGSHFTRFESRLTLTGATGLAAG
                     PGMDISVKNGHNGLLTCRPDQGWLALYGPEQKGAGAIATAILLPAAGNLMGDGGKNVY
                     LVDAVSPDKPLVWYAGAAWSGAGDFVRPEDWTEQVSAFAAALAEPLVVTVR"
     gene            14781..16433
                     /locus_tag="PLZ74_02820"
     CDS             14781..16433
                     /locus_tag="PLZ74_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOR97284.1"
                     /translation="MRTRRWVAVGLMLACACRAESLLRPNDRIIFVGDSITAQAVGEM
                     QGYYHQFTNALKTAYPASRNEVIGLGFSGHTVFSWADSLEVKSRTESVPANTGGFDVH
                     TAFARPADLVLVLLGMNDILRPTVTDEEASLRMWQDKYRELIRAIRKRVSPREFVLCE
                     ITPLTEDPLAPKNRVRDRMTQLVRELAREENCRVAETGGALFDVIARARKARQDYLVI
                     PDTVHPRRPLGHTAIARGMAAALRDAKLTALFDARLDAAITGLRPAQATVTWRFKPQP
                     GIEAKGGEQVYRIECYWDDGTGASVARVPTFSLEVPEGWLVAEAPVTRTEAVFTVRGT
                     PERLQTPLTILAKAGEEVIRRTVQIPVPWRVVCGPENGGAWMSGRRYQPDNRVPFMET
                     DLVAGKHFLEPFTDGGKSYGWQINTPCMDYTAGDDPNAVVPYSLTFGRENDVLYAVRW
                     VYSAKARPVQIRLSHRTFSATLGFVVWINGERVMTADLNRSGKNQAVGQATLKAGWNR
                     LLVRNDHLQWQRQFACALLPVEGDALDDLRYAITPQSVSPSP"
     gene            16741..17703
                     /gene="trpS"
                     /locus_tag="PLZ74_02825"
     CDS             16741..17703
                     /gene="trpS"
                     /locus_tag="PLZ74_02825"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOR97285.1"
                     /translation="MRILSGIQPSGKLHLGNYFGMMRPALRLQEQGEAYLFIANYHAL
                     TSVHDPERLRQDTLDVALDFLACGLDPERTVFFRQSDVPEVHDLAWLLSIVTPMGLLE
                     RCHSYKDKLTKGIAPTHGLFAYPVLMAADILLYQSDVVPVGRDQKQHVEVTRDIAIKF
                     NNEFGEVFRIPEPAISEEVAVVPGLDGQKMSKSYGNTIELFGDPKEARARIMRIVTDS
                     KGLEEPKDPAACNIFALYRLFADPARRAEMEANYRGGNYGYGHAKKALAAIFEETFAP
                     FRARREELQRNLDYVEEVLRKGAARARAEAAKTMDRARRAVGIA"
     gene            17742..18560
                     /locus_tag="PLZ74_02830"
     CDS             17742..18560
                     /locus_tag="PLZ74_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOR97286.1"
                     /translation="MSIEALLPDEYKVDLEVFEGPLDLLLYLIRREEIDIYDIPIERI
                     TAQYLTYLDVMRMLDLNIAGEFLVMAATLMMIKSRMLLPVETRAVEEEESDETWVDPR
                     LDLVRQLIEYKKFKDAANRLLEWETLQGEVFAFGGAQPILERDPPDAGQALGDIGLFD
                     LLTAFQEVLARAPLEPLGHIEPIRWSVPDKMETILTLTRGGNRVAFSALFNPESHRGE
                     VIVTFLALLELLRLRQIRLWQNAAFHEILILPAEEMDGGPLPVPVIEGDEHVQA"
     gene            18547..19341
                     /gene="scpB"
                     /locus_tag="PLZ74_02835"
     CDS             18547..19341
                     /gene="scpB"
                     /locus_tag="PLZ74_02835"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOR97287.1"
                     /translation="MSKPDEVKVPLPASLQQVVGALLFGADHPLTVEEIRTCLKNVAE
                     DASGEEKAALSIFAEASPREIEDAVGGITKELSRLGLGIEVTAVGNAFRFQTQACCGR
                     WVRSLLKAERPSRLSRPALETLAIIAYRQPIAKSEIESVRGVTVDHIVKALMELHLIR
                     IVGRSELPGRPFLYGTTASFLSHFGLGSLKELNDLDPTLQRSKPSERKAIHQKKKKKD
                     ESKDASQDAALPSDPPASEKGATEAVNEILGDGSPPGENAEPLYEA"
BASE COUNT         3840 a         5516 c         6449 g         3634 t
ORIGIN      
        1 actgctgcag cagtccggac ttcgtccaat agctcttgcg gacctgctcc agaatatccg
       61 gagagatcag cgttccggtt tgggcctgga ccgaggcggg ggtgaggagg tgggtacggg
      121 cgtaccgccg cgggatctcg cgcagacgtg tctgcagggc ggggagcgta tccgcgcgca
      181 ggccatggcc gagggccaga tggtagcggt tcagcagtac gctgaggttg tcgggcatca
      241 gcgcttcggt ctgcgcgagc aactccgcag cctcctcgaa gcgcttctgc ccggcgaaca
      301 gaaccgccag ctcattgcca gcgcaggcca gttgtccggc gagcgccgcg cggatcttgg
      361 cgaacaggcg gatgctgtcc ggggttgcgg ggaacaggtg cggttgcagg aactctacga
      421 aagcttggtg ccggtcgcgc agttgggcga ggtctacgga cacgtcttgc ggcagaccgt
      481 tgaagaaaat gctgttgggc aggggcgtga aaccgttctc gcgccagaag accggagtgc
      541 ggaacagggc aatgcgggag taggcgttgg tttcctgggt aagccattcg cgcaggaaag
      601 aaacgggcga aaggtcggcc gcgttcagaa gccgggcttg gattctggta agggagggat
      661 cctcccggat ggcggtgccg aggtgcttcg cgttggcgcc gtccgggctc tcttccgttt
      721 cgatgagccg tagccgacgc ccgtcctgaa aggcgcggag catcaggtga tgcgtgagag
      781 tgctgctgtt gacgatccag tcacaggtgt ccagcgttgc gtagatgcgg tccgtgacga
      841 catcgacgaa ggcgccttca cgcggatcga tgtccgtgaa actgcggaac ggcaccagac
      901 acgcgagtgc caacaggggc cagcagagcc atcggccgat acggcaaacg agaggatggt
      961 cgcggtattc gtaataatcg aggtcctcgg acaactgttc gtcgaacatc tcgcgcatca
     1021 gataccagac ggcaatcaac aggccggtca ggaaagcgac gattgtggaa ctgaagaccg
     1081 gaatgcggga ggtcatacgc gcaatcgccc agggcgacat gcgcaggttg agcaggctgg
     1141 ggatgagtgt ggcggccagc gtgagctgca tgaggaacag cagcggtgtg cgcttgcgga
     1201 aggaaaaggc gaaaatgaac agcgcgatcg cgcaggggaa gagcagttgc atgaagatgt
     1261 agctccagcc gaagctgggc acccagcggg caagttcgtc cagcatggtc gcggtcagga
     1321 cgttcaggat cgggcggggt gccgggatgg ggaccgcggc gcataaggcg ctggcacgcc
     1381 ataggagcat gccgcccgtc agaaacccaa tcagaccggt caacaccacg gctagtgtgc
     1441 ggtaaaaagt ggcgtgttca ttcagcagga gcgatcgcag cagaaatccg ctccctaccg
     1501 gaaggagcaa cagaaagaga ggggactcca cgcagcaggc cgccagcaga aaagaggagg
     1561 cgaacaaaaa gaaagggtgg gctttctggt cataggcgat cagtagattc agaagcagga
     1621 agaacagggc gagatcaaag gcgaagggat agagacgggt cgcggcgatc cagaacggtc
     1681 cgcagaaggc gaaggcgagg gcggcaccca tgccgccgag ggccgcggcg cgcgcactgc
     1741 gccagttatg ggcctgaacc gaggcgggaa ggatcggcat ctcgctggcg ggtagcggga
     1801 aggacatgcg attgcggttc gggagatcgc cctcgtcttc ccgtatgcct tccgtgccgg
     1861 gaggcagggc cgccatactg ccgccgggat tctcagcggc ccaaatgaaa atcagccgtg
     1921 cggtcagcag ataaaagagg gtcgcggcca acgtgccgca ggcggcgcag aagagattga
     1981 gccgcacggg cagggtggcg tacggcaact gcgcgatcgg tcggatgatc agactgaaca
     2041 acggatgcgc cagatcgttt tgctggcaga gaccggcagc cattgcagtc aggaaggccg
     2101 gggttccggg atagaggtga cggaaaagcg tcaccgcata cactacggcc gtcagcagcg
     2161 tcacgaccgg caagaaaaag cggatgagct gccaaggggt tgtgaaaaac ctgtgtttgc
     2221 gggacgccat ggccttgcct cactggtgcg ctctacgacg ccacgcgcgg tcaccgtaca
     2281 cgccagcgcg ggagaaatca aacggacgga agccgatctg ttccgcaggc gaaccgcgcc
     2341 gcagacggaa gtcgcgttcc gcagcgtttt caaacagcgg atccgccacg agcccgtccg
     2401 tgtcgcgccc catggcctgc cattcggcga aggtcatgcc tttaaaatca gctggcccgt
     2461 ccgtccgcca ccacaggttg ctctgccagt ccactttggc ctggatggag cgggtataac
     2521 gggtgtcgct gaagtttggg gcgttttccc agatgatgat gttgcgctcg acggtcagcg
     2581 agcggtgcgg ttctacccgg gtgaccgcca cctggcactc gcggctgaag gcgagaatgt
     2641 tgttgcgcaa cacgttgtca cgaccgtaat gctggtggaa cgatccgtcc ttggtgtcgt
     2701 acacgagatt gttttctaag acgatgccct ccgaaccctc gtccggatag agtccccatc
     2761 cgccgtacgt gtaggagtcg acgttaaaga tcacgttgtt gctgatgcag gtgccgaaac
     2821 tggtccccag cgtgtagacg ccgcccatat cgcccagcat gcgctgcccg atcgtgtgga
     2881 tacggttaaa ggcgatggtg ttgcgttgtg ccgcgctgcc gcggtacccc cagacccagc
     2941 ccacagagac gcccgtgtag aaatggtcgc tgatctcgca gtgggtgacg ctgttgtcgg
     3001 ggctgtggcc gatccagacg gcggtgccgc tcgcgtggaa ccgtccgccg tgagtgatga
     3061 tgcagttgtc gaccgtgttg aacgcggtgc tgaaggatgt cagcgcctga actgcggcat
     3121 tggtgctggc gctcactccc ttggctccgg gatcgccgat gcggatgccg cccgcgccca
     3181 gatcggtgag tgcgcactgc tccacccggt tgctgacaca gcccgcgcgg aaccagaggc
     3241 cgtattcacc ggtgtgcgcg atgtcgcaac gacggaaggt caccccatgc gcgccgtccg
     3301 ccatgatcgc cgcttcggtg tgcgcggccg cttggtgggg ttcgaactgg gtagggccgg
     3361 gcgcgttcag gggaccgcag atttcgtcgg gtagaaacgc gcaggcgatc tggttgaggc
     3421 ggcgcggcga atcggtatag aggaagccga ggttttcaaa ggtgaggtgc cgtacgaaga
     3481 caccggcgac cggatcgccc tgaaagacaa ccagcgtctt gacccccgat aagggagcca
     3541 caacctctgc gcgttcgggc cgctcgtccg gcaatggcag gtagtggagt ttgcggttga
     3601 cgccgtcata gaaccactcg cccggcgcat cgagtgcgga acggacgttc tcgacatagt
     3661 aaagggagtt ggtctgccac ttgttccaat gcttccaagg cgcgccctgg gtgatgagcg
     3721 tcgcctttcc gacatcgaac ccggcgatca tccggcgcgt ggtgtcccat ttgtggtgga
     3781 ccaccacttg ggcgaaacgc cattcaggct gcggactgcg cgccaaggga tcgagttcac
     3841 cggcacggcc ggtcagggtg gcccgggcgt agaccccgcg cggagaggcg ttggtcagaa
     3901 tcgtctgttg cacagcggcg ggattgaaga acccgcggtt gggatgccgc gcgcgcgggg
     3961 cacgccgacc gttgacgaag agggtctcaa aataggcggg tgtgccgctg gcgtcgcacg
     4021 ggatatccgc cagccaccgg ccgtccggct gcacctgcca gccggtgatc acacggccgc
     4081 cgcacaggcg tgaccggtcc gcaccccggc cgcggtaggt gaccggcgcg tcggcggtcc
     4141 cggagtcctc gggactgaac acgatcggtt cgttcacagg gtaaaaaccc gtatcgacat
     4201 gcacggtcca cgcctctgaa caaccagcct tccgcgccgc gcgaatcgcg tcgcgcgcct
     4261 gcgtcagcga agtgaacggg cggcgtcgcg agccgtccgg cggttcagag gttttcgcgg
     4321 cgggagcgac gtacaggtca gcggcgccga gtccggaggc gagcaggcaa cagaacagca
     4381 gcagtgtttt catagggtgt cacgcatcct ggggttaaag gattaacgga aagaggcgag
     4441 gtcgcatttg tccatgtcgt cgaactcctg attctcaccg cccatggccc agacaaaagc
     4501 gtacccgccg gtgcccgcgc cgcaatggac cgaccacgcg ggtgagagta cgacttgctt
     4561 ttcacgcagc atcacatgcc gcgtctcatc cggcgcgccg agcagatgca ggaccgcacc
     4621 gttgggcgga agatcgaagt aacagtacac ctcggtgcgg cggtcgtgcg tgtggggcgg
     4681 gaaggagttc cacacgcttc cctcgtgcaa catggtgaac cccatgacga gctgacagct
     4741 ccggataaat cccggccgga tgtactgata gatcgtgcgg cggttcgact ccgcaagtgt
     4801 gccgagttcg atgcggttgg catccttgag ccccgctttc acggtcggat aatcggtgtg
     4861 cgcgggatag gaaaccagat agaaacacgc cggtttctgg gggttggcgc tagagaagag
     4921 gaggccgcgg ctgccgcgcc ggacgtagag gcattcgcgc cgcgccaagg cgtgctcggt
     4981 accgtccacc accacggttc cgggctcgcc gagattgatg atcccgacct cgcgccgctc
     5041 gcagaaatag gagcaggcca tttcgcgtcc ccccgtcagt tccagatgct cggtttcggg
     5101 aacgatcccg ccgaccaccg cgcggtcggt gttcgtgtag acgaggttga cttggccggg
     5161 ctggaacaga tcttcgatta cgaagcggtt gcgcaactcg tccgtggtca tacggcgata
     5221 ggattgacgg tcaggtgttt cgatgtttct catgatgtct cccaatctac gtttctgaat
     5281 gtcggaacca ctatagcaga ggtcagcccc ccggtccaat acccgatttg ccgggatgca
     5341 ccgcgaaaaa gcggggtcgc accgtgcacg gacctcattg cgtgaagggt tcaatgacgc
     5401 gttcaagaat cccgtgcagg tgtgcgatca gcacaccgta attgacgatc ggcacgcctg
     5461 cggccttggc ctgatggatg cggtagcgca tttcacggcg gttgagcata caggcgccgc
     5521 aatggacgat gagcgcgtaa ggggaaaggt cgctcggata gttcatgccg ctcgtgtgtt
     5581 cgtaaaccag tttgcggccg gtgtgccgga gcagccaacg cgggatcttg actgtgccga
     5641 tatcgtcgca ctgccggtgg tgtgtgcatc cttccgccac aagcacgcgg tcgccgtcct
     5701 tcaacgacgc gatcgcgcgc gtaccgcgca cctgctccgc caaatcaccc ttatagcgcg
     5761 agaaaagaat cgagaaagag gtcagcggca cgttgcgcgg gatttcggcc gccacgctct
     5821 caaaaacctg tgaatcggta atgaccagtc gcggaggccg ggcgagcgtg gcgagcgcgg
     5881 cgcgcagctc gaattcgcgc gtgaccagcg ccagcgcgcc agcctcaagg atgtcgcgca
     5941 cggtttgctg ttgcggcagg atcaggcggc ctttgggcgc ggcggagtcg attggtgtga
     6001 ccagcacaac gaggtcgttt ggtttcagca ggtcaccgac cagccgttcc gcatcgtctt
     6061 ccggcgcgac tttggccagt gccagcttga gatcctctat gcccgcgcgt gtaacggagg
     6121 agaccgtgag tagcggcgtc ttgcactcct cggcggcctg acgcagtgcc gagggcgaag
     6181 gcggccggag gtcgactttg ttgaagaccg tcaacagcgg aatacccttg gcgcgtacca
     6241 gatcgaggac ctcgcgctcg cagtcgccga taccagagtc tgcctccagc accagaatcg
     6301 ccacgtcggt cttgttcagc acgtcacgcg tacgctcgat ccgtagtgcg cccagctcgc
     6361 tctcgtcgtc caaaccgggc gtatcgaaga tcaggatcgg gccgagcggc aggagttcca
     6421 tcgccttgcg gaccggatcg gtggtggtgc ctttgaccgc tgaaacgatg gagacctcct
     6481 gaccggtgag cgcgttgatc agactggatt tgccgacatt gcgccgaccg aagagcgcaa
     6541 tgtgcacacg ctcgccctga gggatatcgt tgaggctcat tgttgtatat ctgccagcgt
     6601 tacagcacgc ggcggtccct tcttgaatac ggtcatttta caggggcacg atccggattg
     6661 tcaacgagag gctgcctttc cctctggcct cgcgcgtttt cctgattgac tcttttgccc
     6721 tccttgtata ataggtgccg actataggaa ccggtgtgtt cccggtctgc cctatgggat
     6781 ggcagggatt cgtgatatga gaaaaatacg ctttcttgtt tgggtcatgg gggcgtcctt
     6841 ggccttcggg cgtggcgaaa gctggatgcc caagccgccc gcaccgcctt cggtagcgac
     6901 gctgacaaat gaggcgcggt acgtcgagtg gaagtttcgc ggggacgtga cagatccgtc
     6961 gacgggcctt gccgcgcacg cgcttcgcac gaacgcgctc gcgctggcgt ccgggctcga
     7021 gccgaaggag gattggcgcg tcgtgaaggc gaaggtctac gcgatgctct gcgaccagat
     7081 ggcgatcgac gtttcgccgc tcgactggta tccggcgatt tcaatctgga atcgatacga
     7141 ccgcccgatg tccgctgtgg tctggcggcg caacggggag atcgcgcgca agtgctaccc
     7201 cgacatcacc gggcggatca acaaaggcaa ccgtgacggg atttggcgtg tctggaagga
     7261 ctttgaccac tctgttcccg tctgggagga tatgttcaag ctcggcttcg ccggaatgcg
     7321 cgaccgcgtg gacgcgcacg atgacgggac gcccttttac cgctcgctcc gcatcacggc
     7381 ggacgcatcg atcagactga tcgaacgttt tgccgcgcag gcgcgcaaac ggctcggtac
     7441 ggcggacgga aaaagctccc gcctcgtaaa acagatcgcc gccttggatc agcttgctaa
     7501 aggtccgccg cagacggctt atgaggtgct cctgttcatt gatctctatt ttttcatgtg
     7561 tgagcatctc gacgccatgc agtgtcgttc gctttcgatt atcgacagga acctgtggcc
     7621 gtactatcag gctgatcttg cggcgggacg gacgaccgaa gctgaatttc gcgagcaatt
     7681 caggcacttc ctctggcaat gggggtcggt tgacaactac tggggccagc ccgtgacgat
     7741 gggcggcaca aaagcggacg gcacgacgga gtacaatccg ctttcgcgga tcattctcga
     7801 tgtgatggac gagtgcgccc tcccgacgcc gaagttccat gtgaagatcg gcaagtcgac
     7861 gccgcgagag atcctcgaca agctcctcag catggcgcga cgcaaccgtt cgctcacgtt
     7921 catcggcgaa gagtcggcgc ggcgcattct cgaacattat ggtgccacca aagaagaggc
     7981 gcgcacgttc tatacgaccg gatgctacga gatcgtgctc gcgaacggcg cgaataagac
     8041 tggtgttggc tacatcaacc tgctcaaaca ggtcgagttc gtcctgcgcg acgcgaaagg
     8101 cggaaagttc tccgcagccg attatgcggc attccaggcg gaatgtatcc gccgtatggt
     8161 gaacgtctgt acgaactgtc tcgcgatcac gagcgcgttc gagcagcatc tggatgacgt
     8221 caaccccgcg aacatcacga cgctcggtgt cgcgtacgcg gtcaaaacgg gacgtgatgc
     8281 ttttgcgaat ggctcggaac gcggaaacaa cactgcgatc ctgatcgctg cgatgggcac
     8341 gaccgtcgac gcgcttgttg cggtaaaaga tcttgtctac gaaaagaaag agatgtccct
     8401 cgcggacctc ggcaaactca tggctgcgaa ctgggaggga cgtgacgacc tgcgtcttcg
     8461 gatgaaacgc tcgaaaagga agtggggcaa caacgatccg gacgcgaacg cgacggcggc
     8521 tgcggtctca aaggcactcg ccgcggcggt caacggcaag ccgaacgcgc gcggcggcat
     8581 ttacgttctc tccggacaca gcgcccgcgc gtttatctac gagggtgcgg caacaggcgc
     8641 gacgccggac ggacgcaagg cgggcgaaga gttctccaag aacatgtccg cgacaatggg
     8701 cgcggatacc gaaggcgtga cggcgctcat ccagaccctc ggcgcactcg attcccgcga
     8761 ttggccgggc gactttccgc tcgacgtgat gctccatccc gcgaccgtgg aaggcgaccg
     8821 tggtctcgcg ctgatgcgga cgctgcttga tgtctactat gcgaacggcg ggcttcagat
     8881 tcaatttgtc gttgtctcgg cggatgaact ccgcgatgcc cagaagcatc cggagaaata
     8941 tgagaacctg cagattcgcg tgtgcggctg gaacgtacga tggaatgata tccccaaagc
     9001 cgaacaggat gcctacatcc tgcgggcgga aagcatcatg caatgaacag aagggaattc
     9061 atcggatctt ccgcggcttt tgctgtggcg ccgcttttcg ccgcaggcgg agggaaagtc
     9121 agaacgccgc tcatgctcga tacgaagcgg atggcgatct gggacggccc cggcgtgcgg
     9181 accacgttct tcgtgaaggg gtgtccgctc aagtgcatct ggtgtcataa ccccgaatcg
     9241 atttcggccc aacgccagac cgcgcgattc cagcatctct gccgacgttg tgaaaagtgt
     9301 acaatggacg aagcgacctg tccgacccgc gcgctcaagt tctacggcaa gccgatgaag
     9361 atcgacgaga tcgtcgcaaa ggcgcttgag gataagccgt tttacgattc gtcgggcggt
     9421 ggcgtgaccc tttctggcgg cgagccgctc cttttttggg aatggctggc cgagcttttc
     9481 acggcattca agaaggcggg gcttcacacc tgcctcgaca cctcgcttta tgcgccgccc
     9541 gtggcgatcg aggcaatgct cccgcttgtc gacatgtggc ttcccgacta caaagccgat
     9601 gacgatgcgc gccatcggaa gtacacaggc gttccgaaca aaatcatccg gaaaaacctt
     9661 gagcgactcg tggcggcgaa ggcgaaactc gaagtgcggc ttcttgttgt gcctaggtgt
     9721 acggatgatg atgatcttgc ggcgcgtcac gcgtatcttg attcgctcga cattccgcgc
     9781 gcggcgcagg tcgaactcga atatcgtgat ctcgcccgct cgaaatatct tgcgctcggc
     9841 atgccggaca cgatgccccc ttcaacgtaa ggggcgggga ggcggatcaa acgccgcaag
     9901 tcgaatgtcg cgggtcgcaa gtcgacgacg ggtacgagta cgaggacggg aaaacgatcc
     9961 cgatagtggt aaacccggaa caaaccgagt taagaaattc attggacggg ttgccgcaac
    10021 acgatgtatt ctgttttcgt cggcttcggg ggtgtgccct gaagcaacag ggatatccat
    10081 tttatgcgtc tacatgaaaa ctgccgtatg aacaacttca aattgggggt cgtggctctc
    10141 gggggagtga tggcgggaac gatactggcg cgtcccgtgc tccagcccga ggtgaaggaa
    10201 tacgtttcgt caaaaggcgt tgcggactat gcggcggcgc cggttgtcta tcaggaccag
    10261 agacagtgcc ggattggtgc ggacgagatt ccgtcgacag gtgaaaagac ggcctatgcc
    10321 gggcatgcgc taccggaggc aggcgtattc atcgctattg ccgattcggc gctcggcaag
    10381 aagttggtcg cggatttcag gctggacgtg ccggcgaaga aacagggcta cgcgatccgc
    10441 gtcgacggga aatgcgcggt catcgtcggg cacgacgcca tcggtgcgct ctatggtgcg
    10501 gtgacgtttc ggcagatggc ggcgaaggga ggcagggttg agtgcgcgat ggtgcgcgac
    10561 tggccagacg tgctctatcg cggtggagct tcccttgggc gtgggctatg gccgtacggg
    10621 aagtccgacg gtgttgacgg catcaaggcg gcgctagacg tgatgatgcg ccacaagctg
    10681 aatatcatca gcgacttttt cggactcgat gagttggcgc ctcgcgagaa actcgcgact
    10741 tggcgcgaac ttgtgcgata cgcgcgggac cgcggcatct attcctatta cttcatctcc
    10801 acggcgcttt ttcgccgcac caacaagcct gagggcatga ccctcgagaa gtggccgtgc
    10861 gtcaaggacc tccgtacctg gaacgaatgg tattactgct ggtccgacga cgagggaacc
    10921 gagaaggccg tcaaccactg cgcagacatc ctgcagaaca tcggcgcgga ggacgggatt
    10981 gttcaaatcc atcccgtcga cggaggaggt tggaaggatc ccgagttctg gagcaagcgt
    11041 tgcgcgaaat gccgcagtct ctggaatgac cacgagcggt ggaaggcgac ggtgcaccag
    11101 ctagagattt ggaaccgtgt gctgcggcgg agatgtccga atgtgatcgt cggttcctgc
    11161 atctatccgt acacatacag tgcgctgctg acaccggaga aggaccgcac cgacctctgg
    11221 agagagtcac atacggaata ctggtcaaag gtcagcgaag ggatcggaaa caaggagttc
    11281 tttttcagca gttggatgtc tccgccatgg ctggccgaac agattcggcg atatgtgcca
    11341 tcgcgtccta ttcatttcgc ccagtatccg cagttctcag gcatcttcca gacaacttcg
    11401 cgcaaggcat gctccgtgta cgaggagaat gccgataaca cctatcttcc tcagggaacc
    11461 gacttcgacg cggtccaatg ggaatctcta ctgttgggtg tcgaatccac atggaaccga
    11521 aacatgcccg gggcggaggc ctttgacggc ggaacatatt atgacgcggc ggttgaccat
    11581 acggggccga ggatcgtgat ggaacaggtg ctgccgcgcc tctgtgcggc tttttgggga
    11641 acggaactcg cgccgtacat gacgcgtatt ctgtcgtcgg gcgtgttgcc gtcctacatc
    11701 cgggatccgg gcaaggcgct tgcctactgg aattccgttc ggaaagatcc gctcttcgat
    11761 ccgacggtag aacaggtctc gactctggaa cggaagcgca tcgagaaaat cgtggacacg
    11821 tccgagcgga tgcgtgagca ggtgaaggct gcggaaaccg tcattcgaga cctgacggag
    11881 gcccgcggtc atctggacgg gttggactcg gtccgtcgcc ggtatttcat gaagctggcc
    11941 aagtatgccc cgttctggct ggccaccgct cgcgcccgat atgccttgcg cgcggcgaat
    12001 gacctgattg ccgccggtcg gcatgaggag gcgaagaggg ccctgccgga ggggctgcgg
    12061 caactgcggg aggagtggcg tcaggcggag gagaacctca aggctctatc cagtgaggtt
    12121 gactgctctc cccgaaggta cgtatggccg gtcaagctgg agacgttggt tgctgaattc
    12181 gagcgtgcgg aaacctcagc ggggcgtcca tagttgcgtg ccatcagcgt ggagccggac
    12241 cgccgaaacc gtgccgggcc ttcgcggcag ggatgtctct ccgaggagac gcccgagtac
    12301 ggggacgaaa aaacgatagc gatcctgatc gtgattacga tggggcgcgc gtcccactca
    12361 aaaattttga tgcgcatggc tttcgcgcga ggctgtacga agggagtcaa aaacgctact
    12421 atccggttac tgaatcagta cggcgcaatg cagccgttgc acaaaccgga gggtacgatg
    12481 gcttcacgac ggatgttttt tgctctgatc attctgttga gcgcgatccc ggaccgcact
    12541 gcgcgggcgg tttcagcgga gtggacattg gaaacggttg accggaatgc gcgcggggtc
    12601 gccgattggg cgcgccccaa tcccaagcgg agtctcgtct gggaaaacgg gacgctgtac
    12661 gcggggatca cggcgcttgg actcgctgat ccgtccttgc cataccttga tcagatccgg
    12721 cagcttggtg cgcatcatgc gtggggtcat cgggagcgca cctaccacgc ggacgaccac
    12781 tgcatcgggc acgcctggct ggaactggcg gcattcgaca ataacccggt ctatgccgcg
    12841 cggctgcgcc ggaatttcga atacatcctc gcgaaccgcc accggggttc ccttaactgg
    12901 tcggagaagg attgcgctaa acgctggtgc tggagcgacg cgcttttcat ggcgcccccc
    12961 gtctgggtca agatgtacgg actcaccggt gaccgccgtt acctcgactt tttggatcag
    13021 gagtacaaac tgacgaccga tttgctctat gacaaagagg cgtcgctctt ttaccgcgat
    13081 tcacgctatc tgaccgagcg gacgaaaaac gggaagaaag ttttctgggg tcgcggcaat
    13141 gcctgggtct atggggggct ggcactcatg ctccgtgacc tgcctgccga ttggcctgcc
    13201 cgggcagact acacaaagct cttccgggaa atgtccgcag ccctgaagaa aacgcagggg
    13261 acggatggcg cgtggcggcc cagtctgttg gacccggatg accccgacct gcccgagatg
    13321 agctgcacca cgtttttcgt gtatgggatg ctctggggtg tcaacaacgg cgtgctcgat
    13381 aaggagacgt acctgcccgg catcaagaag gcctgggccg tgatcagtgc cagtatcaac
    13441 ggggagggtc ggctgggctg gactcagccg ccgggttggg cgccggaaaa ggccttcaac
    13501 gcggacacgc atgcggttta tgcgatcggg tcttatctgc tcgcggccat ggagttgcgc
    13561 aagcagattg tggcagatgc gcaccccgaa cggaaaaccg tgacggcgac caatccgtcg
    13621 gagcgcttcc ggagtgccga aaccctctcc ctcaactggg gggagctgaa actcgacgcg
    13681 gccaagacgc gcgtcttcga tgtccgtaat gccgcctttc tgccgcatca gctcatcgac
    13741 agcgatctcg acggcgtgcc tgacgagctg atcttccgga gttatttcgc ggcgcgccag
    13801 acccgggagt tttgggtttt cgaggacagc ggcattccgc cgcctgtttc cccggagatc
    13861 tgcttcagtc ggcatgtccc ggagcggatg gatgatttcg catgggagaa cgaccggatg
    13921 gcctaccgga tctacggacc cgccgtgtcg ctgccgccgc cgcagggcga ggggttgcat
    13981 tccagcggga tcgatgtctg gtgcaaaaaa gtccggcacc cggtgatcaa cacttggtac
    14041 aaacacaggc ggtatcatga cgatcacggc gaaggcatgg atttctacaa ggtcggtccg
    14101 ggccgcgggt gcggcggatt gggtgtgttc cacgcgggcg cgttccatgt cagcggaaac
    14161 tgggccacat ggaagcacat cgcgaacggt ccggtgcgga ccgtgttcga agtgacctac
    14221 gcgccctggg cgtgcggaaa cggggcgcag gttgcggaga cgcggcgagt gagtctggac
    14281 gcgggttcgc acttcacccg gttcgagagt cgccttaccc tcaccggagc gacgggtctg
    14341 gcggcgggac ccggaatgga catctcggtg aaaaacgggc ataacgggct tctgacgtgc
    14401 cgtccggatc aggggtggtt ggcgctttac gggcccgagc agaagggggc cggggccatc
    14461 gccaccgcga tccttctgcc tgcggcgggg aacctgatgg gcgacggcgg taagaatgtc
    14521 tatctggttg acgcggtgtc ccctgataag cctctcgtct ggtacgcggg agcggcttgg
    14581 agcggcgcgg gggatttcgt caggccggag gattggacgg aacaggtgtc cgctttcgcc
    14641 gcggcgctcg cggaaccgct tgtcgtgacg gtccggtaaa cgcgcacatg ttctgtaaag
    14701 aagaggaagg atgaggcggt tgccgacagg gaccgggtgc ggctcggacg ggtgccggag
    14761 agtaaaagga ggagaagcgg atgagaacga ggagatgggt tgcggtaggg ttgatgctgg
    14821 cctgcgcgtg tcgcgcggaa tcgctgctga ggccgaatga ccggattatt ttcgtaggcg
    14881 acagcatcac ggcgcaggca gtcggggaaa tgcagggcta ttaccatcag ttcaccaacg
    14941 cgctgaagac ggcttatccc gcttcccgta atgaggtgat cgggctggga ttctccgggc
    15001 atacggtctt cagttgggcc gatagtctgg aggtcaagag ccggaccgaa tccgtaccag
    15061 ccaacaccgg cggcttcgat gtccacaccg cgttcgcccg tccggccgat ctggtgctgg
    15121 tcctgctcgg tatgaacgac atcctgcggc ccacggtgac agatgaggaa gcgtcgctgc
    15181 ggatgtggca ggacaagtat cgcgaactga tccgggcgat ccgcaagcgt gttagcccgc
    15241 gcgagtttgt gctgtgcgag atcacgccac tcaccgagga tccgctcgcc cccaagaacc
    15301 gtgtgcgtga ccggatgacg caacttgtgc gtgagttggc gcgcgaagag aactgccgcg
    15361 tggcagaaac gggcggcgcg ctcttcgatg tcatcgcgcg tgcccgaaag gcgcgacagg
    15421 actatctggt gattccggat acggtccatc cgcggcgtcc gctcggacac accgccatcg
    15481 cgcgcggtat ggctgccgcg ctacgggatg caaaactgac cgccctgttc gacgcgcgcc
    15541 tcgatgccgc gatcaccgga ctccggcccg ctcaggcgac cgtgacctgg cgtttcaaac
    15601 cccagcccgg catcgaagcc aagggcgggg agcaagtcta ccggatcgag tgctattggg
    15661 atgacggcac cggggcgtcc gttgcgcgcg tgccgacctt ttcgctggag gtgcccgagg
    15721 ggtggctcgt ggccgaggcg ccggtgaccc ggaccgaggc ggtgttcacc gtgcgtggca
    15781 cgcccgaacg gttgcagacg ccgctgacga ttttggctaa ggccggtgaa gaggtgatcc
    15841 gccggacggt gcagatcccg gtaccatggc gcgtggtctg cgggccggag aatggcggcg
    15901 cgtggatgag cgggcggcgt tatcagccgg ataaccgtgt gccgttcatg gagacggatc
    15961 tggtggcggg caagcatttt ctggaaccct tcacagacgg cggaaaaagc tatggctggc
    16021 agatcaacac tccgtgcatg gactacacgg cgggcgatga tcccaatgcg gtcgtgccct
    16081 acagcctgac cttcggcagg gagaatgacg tgctctacgc tgtgcgttgg gtgtacagcg
    16141 ccaaggcgcg cccggtgcaa atccgactgt cacaccgcac cttctcggcc acgctggggt
    16201 tcgtggtctg gatcaacggt gagcgcgtga tgacagcgga tctcaaccgc agtggcaaga
    16261 atcaggcggt cggccaagcc acgctcaaag cgggctggaa ccgtctgctg gtgcgcaacg
    16321 accacctgca gtggcagcgc cagttcgcct gcgccctgct gccggtcgag ggcgacgcgc
    16381 ttgacgatct gcgttacgcg atcacgcctc agagcgtatc cccgagcccc tgaccgttgc
    16441 gggttcgggg ggcgggcttt cagtcgggaa cacgggcgtc cccgcccgta atggcggatc
    16501 agtcgccctg ccaaacaacc caaatgccgc aagtcgacgg gtacgagtac gaggacggga
    16561 aaacgatccc gaatccgatt tcgatagcga taaacccgga acccgaaacc accttcccgc
    16621 cttcgctcgg gaaaggtccg cggcgttttc ttccaagtcc gttcgggact tgtctaatcc
    16681 gttgtggacc ggtatgatat tcattttccc tttcggcaaa tgatgaaagt tcggcacgat
    16741 atgagaattt tatctggaat ccaaccttcg ggcaaactcc acctcggcaa ctatttcggg
    16801 atgatgcggc ctgccctgcg gctgcaagag cagggcgaag cgtatctgtt catcgcgaat
    16861 taccatgcgc tgacctcggt tcatgacccg gaacgtctgc ggcaggacac gctggatgtg
    16921 gcactcgatt ttctggcgtg cggtctggat ccggagcgaa cggtcttttt ccgccagagc
    16981 gacgtgccgg aggtgcatga tctggcgtgg ctgctctcga tcgtcacgcc catgggactg
    17041 ctggagcgtt gccactccta caaggacaaa ctcaccaagg ggattgcgcc aacgcatggg
    17101 cttttcgcct atccggtcct gatggccgcc gatatcctgc tctaccaatc cgacgtcgtg
    17161 ccggtcggac gggatcagaa gcaacatgtg gaggtgacgc gtgatatcgc gatcaagttt
    17221 aacaacgagt tcggcgaggt cttccgcatc cctgagcccg cgatctccga agaggtggcc
    17281 gtggtgccgg gtctggacgg ccagaagatg tccaagagtt acggtaacac catcgagctg
    17341 ttcggcgacc cgaaggaggc gcgcgcgcgc atcatgcgga tcgtgacgga cagcaagggg
    17401 ctggaggagc ccaaagatcc agccgcctgt aacatctttg ccctctaccg gctcttcgcc
    17461 gatccggcgc gacgcgcgga gatggaggcg aactaccgcg gcgggaatta cggatacgga
    17521 cacgccaaaa aggcgctggc cgcgatcttc gaggaaacct tcgcgccgtt ccgcgcacgg
    17581 cgtgaggaat tgcagcgcaa cctcgattat gtggaggagg tcttgcgcaa gggggccgcg
    17641 cgggcgcgtg ccgaagccgc gaaaaccatg gaccgggcgc gccgggcggt gggcatcgcg
    17701 tgaccgatgg cgcggggccc gcgccgcgga gaggggtaga catgtcgatc gaggcgctgt
    17761 tgccggatga gtacaaggtg gatctggagg tctttgaggg gcctctggac ttgctgttgt
    17821 atctaatccg gcgcgaggag atcgatatct atgacattcc gatcgaacgg atcacggcgc
    17881 agtatctgac gtaccttgat gtgatgcgca tgctcgacct gaacatcgcg ggcgaattcc
    17941 tggtgatggc cgctacgctg atgatgatca agagccggat gttgctgccc gtggaaacgc
    18001 gcgccgtcga ggaagaggag agcgacgaga cttgggtgga tccgcggctg gatttggtgc
    18061 ggcaactgat cgagtacaag aagttcaagg atgcggcgaa ccggctgttg gaatgggaga
    18121 ccctgcaggg cgaggttttc gcgttcggcg gcgcgcagcc gattctcgag cgcgatccgc
    18181 cggacgcggg tcaggctttg ggtgacatcg gtctgttcga tctgctgacc gcctttcagg
    18241 aggttctggc gcgtgcgccg ctggagccgc tgggccatat cgagccgatc cgctggtcgg
    18301 tgccggacaa gatggagacc atcctgaccc tcacgcgcgg cgggaatcgc gtggcgttct
    18361 ccgcgctgtt caaccccgaa tcgcatcggg gtgaggtgat cgtgacgttt ttagctctgt
    18421 tggagctgtt gcggctccgg cagatcaggt tgtggcagaa cgcggcgttc cacgaaatcc
    18481 tgatccttcc ggcggaggag atggatggcg gaccgttacc cgttcccgtg atcgaaggag
    18541 acgaacatgt ccaagcctga tgaggtgaaa gtcccgttgc ccgcaagcct gcagcaggtg
    18601 gtcggggcgt tactgttcgg cgccgaccat ccgctgacgg tggaggagat ccgtacttgc
    18661 ctgaagaatg tggcggaaga cgcgtccgga gaagagaagg cggccctgtc gatctttgcc
    18721 gaagcctcgc cgcgtgagat tgaggatgcg gttgggggaa tcaccaagga gttgtctcgc
    18781 ctcggtcttg gcatcgaagt cacggccgtc ggcaacgcct tccggttcca aactcaggcg
    18841 tgttgcggcc gctgggtgcg tagcctgctc aaggccgaac gccccagccg actctcgcgg
    18901 ccggcgttgg agacgctggc gatcatcgcc taccgccagc cgatcgccaa gtccgagatc
    18961 gaatcggtcc gcggcgtgac ggtggatcat atcgtcaaag ccctgatgga actgcacctg
    19021 atccgtattg tgggtcgcag cgaactgccg ggacgcccct tcctgtacgg tacgaccgcc
    19081 tcattcctca gtcatttcgg gctcggcagt ctgaaagaac tgaacgatct cgaccccacg
    19141 ctgcaacgtt ccaaaccctc tgagcgcaag gcgatccatc agaaaaagaa gaagaaggat
    19201 gagtcaaagg acgcttcgca ggacgcggcg ttgccgtccg acccgcccgc ctcggaaaag
    19261 ggggcgaccg aggcggtcaa cgagatcctc ggcgatggct cgccgcccgg agagaacgcc
    19321 gaaccgctct acgaggcgta aacctaaaaa gagcagttga accttcaact ctctttggtt
    19381 tcaaggtttc tggtttcatg gtttcagggt ttcatccaaa tcggggtcgg gatcgctat
//
LOCUS       DAOSRB010000029        19111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3035_length_19111_cov_7.390271, whole genome shotgun sequence.
ACCESSION   DAOSRB010000029 DAOSRB010000000
VERSION     DAOSRB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19111
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3035_length_19111_cov_7.390271"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1457)
                     /locus_tag="PLZ74_02840"
     CDS             complement(138..1457)
                     /locus_tag="PLZ74_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97288.1"
                     /translation="MSISARTTKAVLCFSRVLAFGCGVALQQIAAFGEPASLTGSARD
                     EVVSRFRLGPFFEYRATREGGVFWAVRPFYADVRDPVSDTHVTDVAWPLGTFHRDHQQ
                     SWWRLLLAYGSDDDVTRDDAAWKTVILPVWFEGRTRAGEDYWALFPLYGHLPHMALMD
                     DIDFTLFPLYFNYEVNGVERVYTPWPFFSRLTENPHLREEGVFPFWCRQINTKKKTDR
                     RYVLWPFWNSARYEDERNPGSSFLLFPLYGEVNRARESQRLVLPPFFSYARTDSAERW
                     RLPWPFYETREIWETRTTEASFQRSYWPFYGEFEGSDERRRYAAWPLLKYTRTESPKR
                     LTERTRFFPFYTCETIWQPSPGAPGLRETERYTRVWPFFVRETTSRGSRFRALEFNLI
                     RHSGGIERNWAPFWTLYERVELPSGMARHDLLWGLVRWTTGRRGGSE"
     gene            complement(1432..2514)
                     /locus_tag="PLZ74_02845"
     CDS             complement(1432..2514)
                     /locus_tag="PLZ74_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOR97289.1"
                     /translation="MIRNMSDITWEAFEPLPFFEKATYLMQENGMPYHTLFAQQFGYE
                     LLDKLATLANEIRSIAKTREGTGFLREQASHKRAMLYFSQPSSRTFLSFVSACQILGV
                     PTGEVRDTSISSEFKGESREDSVRTFSSYFDLIIMRTPEKGLAERMAWALSNSERPIP
                     IINAGSGQDQHPTQALLDIYTLLRSFERSGGLRDRTVVFCGDLLRGRTVRSLSYLLTN
                     YPNIRQIFVAPPSLQVCPDVLAMLKGKDVTFELTDDFRAAIRQADAVYMTRIQDEWDA
                     AKGDSDKIDTRQFKFGLDELKILPQHAILMHPLPRRDEISKSVDSDPRAMYWRQMRNG
                     MWIRAALIAVTFGCEKHIHEYFRQND"
     gene            complement(2563..3567)
                     /gene="argF"
                     /locus_tag="PLZ74_02850"
     CDS             complement(2563..3567)
                     /gene="argF"
                     /locus_tag="PLZ74_02850"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOR97290.1"
                     /translation="MLNWMPELYGRSLLRMVDLSDEEMLNVIDLAVALKARRRAGIRG
                     DLLSRRHVALIFEKSSTRTRNSASIAIRDEGGTAEYLTKNDLHFGAKESVKDTARVLG
                     RLFDGILFRGYAQQTVETLAAYAGVPVWNGLTDVFHPTQILADLMTIQENFGRLKGLK
                     VAYVGDGRNNVANSLMFGCAKAGIHYVNCTPSDLLPDPDLVREAGEIARRHGAGVEVV
                     TDPVRGVAGANVLYTDVWVSMGEEAQKEKRLRLLRPYQVNRALVRATGNEDGGLIFLH
                     CLPAFHDHETTVTAKSGALEVTDEVFEAPYSKVFDEAENRMHTIKALYVSALTDLSAL
                     "
     gene            complement(3638..6802)
                     /locus_tag="PLZ74_02855"
     CDS             complement(3638..6802)
                     /locus_tag="PLZ74_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97291.1"
                     /translation="MSGTARLIGITMLAACVAAGEGTLAYWPLDTFRSEADLRDIAGL
                     HSLAATGSCAVTPSFRMPLAVLPAYESLPAASRGEGRNRGSVLFSGVPAAELRAAGLG
                     RALVWTNAFTVEGWVRCADDPAEGVSWPLFGAGRPGTGWRVALCRDAQGPAFVLHADA
                     AADRLTLRHRFRDVTKDASYVWRHMCLTYDGTGAGCGVWEFFVNRVSLGVATNPVRAA
                     CADLPGGFFLGGAGEADDAFYGQMDLWRVSAGVLPPAAWLCAAVPRTLAYWPMDRLPN
                     GTPDLRDLTGGPYALAPGRDGGISVAPEQVSGRLPTEPGLWRRSRPARANTGSVRLDG
                     ALGQRSLLVAPDLGLRCDLTNRFTVEGWFRKSANPTERFWQVVGARDNSNGWMLSLRP
                     DAAAVRFHLHVSDVAQGGRLQFERSFVNADVTACTNWCHLALTYDPLRNGVGVWELFL
                     DGVSQGSVANPAPPDRSHGWRDLLLGGRPSLSNSFSGWLDCWRVSDSVLAPEQFLCHA
                     ADTVAAPRPERYTDVRRLDGSMMLPFQRARGRPFCMVLRDGSWLAVQGVEPVPPRSGF
                     CDVAVSVSRDRGKTWSAPVMPVTVKGGEEVSAIGYQTPFGRIYLFWTDEAVRGYGFLF
                     SDDGGVAWSTERLRLTSAAAGQTGGGMPVGIGVPVSLADGAVCLPLVMRREPDGAIEG
                     RVAFSRNLYSERNAARVRFEVTESTGPGFRAMTDPGEGAFYCLAAPDGKTVCGIAGSA
                     QGPVRCANPDGKGNGMPLEKLRFESGQTLAAVTGHVSAAVLSGDRMLLLWRGRSGERC
                     GRGGSVWGSGARQIGDPKTRWSEPELLLYGDGAGGEVCLGSVWEQDNQCWLAVRQGRV
                     VRAYPLDRLRTDGLWPDPMARSVSTNGLVFAYGGTGPTVVPAAFGAHGRPGWSVALWC
                     ALADAEPGAVLFSTWNGRRGLRVSMARSRGAPSLRLEAYDGNRRAVCTTAPGALTAEK
                     THQVAFVCDAACGRMWALAEGRFPEGGTDGGEGTAELPEGFGAVDVPGPATIAGRVSG
                     LQLYDRPLRTAEVVGLARTGPIAP"
     gene            complement(6873..7496)
                     /locus_tag="PLZ74_02860"
     CDS             complement(6873..7496)
                     /locus_tag="PLZ74_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOR97292.1"
                     /translation="MSIEPIREALALGKAAEVTQSVTLALEQGVDASVILNDGLCQAM
                     GALGERFRKGEVFVPQVLLAARAMQKGLEVLKPHLVAAGVEPVGTAVIGTIRGDLHDI
                     GKNLVGMMLEGAGFRVVDAGVGVTAATFVELARTHGARIIGVSALLTTTMVHMREVVE
                     AARSSGLDVRVIIGGAPVTQAFCDEIGADGYSPDAASAAQLALRLIR"
     gene            complement(7533..9197)
                     /locus_tag="PLZ74_02865"
     CDS             complement(7533..9197)
                     /locus_tag="PLZ74_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97293.1"
                     /translation="MADTQNGKEDTRQWFLRINGETVFGPVSTEGLIVWAEQGRILPG
                     HEISTDRKKWVQAVSEPLLDMCWFVDDGMGELRGPLNRCAAEALLKSGKVPEGSVLVS
                     TEELEADASADLSSAPRPGPLRSAAPDAEVLERNRELEAQVAELRERLARLSESDALE
                     TLRRECGTLAGQVQEVTEQRDSLLRNAEKDARANERKIEGLKQQIRKLEQRCEEADGR
                     LLLSDAAAKQAQALQRAEAETQAKCAAAEKELVTVREELAALSVTCERLRGATEECET
                     LRTRLAEAEAQASEAKERLTVLKGERETLEAALREQEVAFAELLTDANNRDNAYQEQI
                     ATLTQACALSPEETTRFYEDQAALFELMKSEADALAERLESERTHLEQLKTWSTQRQQ
                     FLSELRQTLLQRLGVTPADMTRRALRGQPSDPNANRLRTEFDNMKVAYEREVRLATER
                     ERDLQRKIRVMEAEAGKWRERAAVGDKLGRRLQEMEEVLQNREQELADERKSREAERE
                     QYQGNQQALLLRIEALEKASRPVTPDDVQAAEARAVKLASWMRLKR"
     gene            complement(9265..10461)
                     /locus_tag="PLZ74_02870"
     CDS             complement(9265..10461)
                     /locus_tag="PLZ74_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97294.1"
                     /translation="MGKRKGGEKAVWRVRNEQGEQFGPVDFQTLQTWAADGRLAPTSE
                     ISENGTVWQRVTTRAELGMDWVAEVTPGAFYGPIHGQAMRDLVQEGSLSGETAFFTRQ
                     PLDAADRARLEEEARAALTRERDAERERARGLEEQAARLRAERKAWEIEGESLRQRLT
                     QAGEEVKALRDREAAAVAAAADQAQRFEEERGQLREAVKCAERSLAEQATRIASLEGE
                     LADARREADQNENVHRRVQAMEEEAAGLRREVTEAAAQLSERQSRLDALQSETDALRS
                     EAADTRRRVEAARALARQAAEALDSVPSEGDAVRLEAEPVDEPAVRKTEAGTRNSREV
                     PRETQVIEPEVLAPEQKRPSHGKPPVIRVASQAKPGLSLAELERQARRELERLGERGK
                     SFFKKK"
     gene            10642..11403
                     /locus_tag="PLZ74_02875"
     CDS             10642..11403
                     /locus_tag="PLZ74_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97295.1"
                     /translation="MNACSSTPFLKRPTAWMAAPLLWIAGVVVLSVIGLTATPDWIAN
                     HFDQDGASPVEAATIALFWLQIAFFWLVPPMRPGRWRAFWLTNFSLLTFFAICRQLDW
                     HRLLVTPSGLPGTTRGTPFKMKFLLNARNPLPDRILVALCFVLVIALCGGTLLYFLPR
                     LLRGLFRMHPVCWTIGFFGGTLILIQIFDRMSSVLRKDFGIHLTDRQHALTTALEEGL
                     ELLLPLFIILAVLQAHFIYLDSDAESGPLARHRAL"
     gene            11441..12130
                     /locus_tag="PLZ74_02880"
     CDS             11441..12130
                     /locus_tag="PLZ74_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR97296.1"
                     /translation="MRILVIEDDDKIQSFIGRGLSQEGHTVDMASTGTEGLRLWQCTQ
                     YDAVVLDIMLPERNGIDLLRQMRHKGDPTPVLVLSAKSAVEDRVTGLEIGADDYMTKP
                     FSFSELIARLHAITRRMQSPAQSGKDATSLSLGGVTLDLLRRTVVRENRKIELQPREF
                     ALLELLMRNANRPLNKTVILERIWDYSFDPQTNVVDVLVCRLRTKLDAGFSVKRIQTM
                     RGIGYVFRTTE"
     gene            12108..13538
                     /locus_tag="PLZ74_02885"
     CDS             12108..13538
                     /locus_tag="PLZ74_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOR97297.1"
                     /translation="MFSARRNNRLRSFAMYLGMGYLIVYALCTGVIFFLITRVITVSA
                     RGFDREDVLAESEELVELLTQNTDGNLLAEQVTMGRYPPSTIFIVRVINRRGLVEYTI
                     TWPKKIDLPDWRAFAEGDENLPREGLSEYYIRPLKRHVQIQTTHLPDGRVLQVGKGSF
                     LEVDQKSMLVRILLIYAALSTFFSIASGIFMMLITLRPIHRITKSMSDIIRSQAFETG
                     APPVRSLIAELDTLGQLFTIMTGKYANLIRAMRETMENVAHDFRTPLARIRGASELAL
                     HREHLPAELTETLADIIEDCDRLQLQLQNLMDAREMESGFVRLNRQPFRLNQLLGEIV
                     DLYSLLAEEKRLTLSYVNACDRDVLFDGDRNRLARAFANLMDNALKYTPNGGRVDLTL
                     ETGPDAVRVCVRDTGIGISEAEQTLVWQRLFRGKQARETEKGLGLGLNIVQIIISAHG
                     GTVTLESRPGAGSVFRVTLPFRDAPS"
     gene            complement(13601..14335)
                     /locus_tag="PLZ74_02890"
     CDS             complement(13601..14335)
                     /locus_tag="PLZ74_02890"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOR97298.1"
                     /translation="MTGKGRQLAGLLAEWLYPRVCPNSSCGKPSDRPGRSLCWECFRT
                     VELYRESLCERCGAHAEGRVGHAFECGVCREVRPHFDRARSAGRFRGTLREMIHGFKY
                     GKALWLCQDLTDFLAGCLTAHFDPVAVDVVVPVPLHPVRERERSYNQAALLARALAER
                     IGRRYDDRALRRMRHTETQTHLDAVRRRLNMRGAFEVVRPAWVTQRCVLLVDDVMTTG
                     ATLDACAHALKRAGARTVWALTVARG"
     gene            complement(14349..15254)
                     /locus_tag="PLZ74_02895"
     CDS             complement(14349..15254)
                     /locus_tag="PLZ74_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOR97299.1"
                     /translation="MKPTLVILAAGMGSRYGGLKQVEPVGPSGETIMDYSVFDAVRAG
                     FNRVVFVIRRDFEKAFRETIGAKYAGAVEVDYAFQALDDLPAGFTVPAGRTKPWGTAH
                     AIRSARQVVEAPFAAINADDFYGQDAFARLGAFLSASRPAPKPRFAMVGYRLDQTLSE
                     HGGVARGICRVTPEGMLAGVTEMTRLTRVADGVENQEDPARPVRLNGTERVSMNLWGF
                     TPALFAALEERFPAWLAAHAADPKAEWYIPFVVDGMIREGAAEVEVLPTESAWFGVTY
                     REDRPAVMAAVQARVRDGVYPDKLW"
     gene            complement(15412..16170)
                     /locus_tag="PLZ74_02900"
     CDS             complement(15412..16170)
                     /locus_tag="PLZ74_02900"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOR97300.1"
                     /translation="MRLLVMDVGNTSTGIGLYDRGVISRVTHVRGGIVKRPAACAAAV
                     RKAIRGGIHGAVLGSVVPRANAAWRRLVRNETGCAPLVVRAGIPMEVTIDYPAPETIG
                     ADRLAGACGGVRRYGAPLIVADFGTALTFDVVTPDRRYVGGVITPGLPLMTDYLYERT
                     ALLPRVNLEGPCPGIGRSTLQAMRVGAQIGYRGIVREIVGHLRQTMACDFKLVATGGY
                     AGWALQESGMEFVLDPQLTLFGLGCIGESNGPGG"
     gene            complement(16174..17040)
                     /gene="nadC"
                     /locus_tag="PLZ74_02905"
     CDS             complement(16174..17040)
                     /gene="nadC"
                     /locus_tag="PLZ74_02905"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOR97301.1"
                     /translation="MEGDGVMGDPRAVAVVRAALEEDIDTGDATTRALVDPQIEATAV
                     LLTREPCRVAGVGVGQTAFLCVDASLRIETLRPDGHAAAAGDVLMTLRGRAASILTAE
                     RTALNFMQRLCGIATLTARFVEAVKPYGTLILDTRKTTPNLRLFEKYAVTCGGGVNHR
                     FGLYDRVLIKDNHRRLWLGGDPDRLDLAVAAARKAYPALAVEVEVESEAECVSALRGK
                     PEWIMLDNMDCGLMRTCVALCRGKAKTEASGGITLDRAREVAATGVDAISLGCLTHSA
                     PAVDLSLEWSWD"
     gene            complement(17030..17809)
                     /locus_tag="PLZ74_02910"
     CDS             complement(17030..17809)
                     /locus_tag="PLZ74_02910"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOR97302.1"
                     /translation="MRRGVWRLRGFARSAPAAIGLVAVCLGVFLIQQTAARVDFVAVY
                     GYRYGYDQALLHCFGLSWPLLREGFVWQLVTYMFLHASWQHLAMNMLTVLLLGSGLEA
                     EIGGRRFKWIFLTGGLLGGLGWLMVTALTPLLPSAEALTQWLPRTAAAWLQAGASSGQ
                     TLDVALCIGASGGVFALIGAYSALFPRREVYVLLLFFPLRLKARTLAWFLGAVTVAEA
                     VFVQSRVAYAAHLAGGIAGYLCGCALRTEAGDWRGGVPYGG"
     gene            complement(17841..18065)
                     /locus_tag="PLZ74_02915"
     CDS             complement(17841..18065)
                     /locus_tag="PLZ74_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97303.1"
                     /translation="MKLKKSSIEAVEETKPATGGAFIAARHRNPVAEVAARQAAGQEM
                     FGSVCAILATVVLLIITALLYVNWNAIKSA"
     gene            complement(18174..19001)
                     /locus_tag="PLZ74_02920"
     CDS             complement(18174..19001)
                     /locus_tag="PLZ74_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97304.1"
                     /translation="MYLTTFRVGEVARVIWSLRHAGVEIGRGCLRFLREPVAVLPDVV
                     SGGLLKVGPDFVVLVASRTARDTEPLSVPQIGTNGVAVLDGFLYGGTQNVECRTLNAE
                     PGNEKPEIRFRVVDMQAVEQSETATGMTLLQPFTVLRSVLPADTVVVAPSLLAISREG
                     GTEGFERRLAAMTGLLSGPACGSPRVLLTVPPAFDVLPGCGCVPGGKPCEHAAAARSY
                     AESVMRVADAYGVETVDLFTAAKTAEGAVPLVCKGELTPEGRALFEMLLEKKGVSLR"
BASE COUNT         3620 a         6969 c         5252 g         3270 t
ORIGIN      
        1 gatagcgatc ccgattccga ccccgatttg gatgaaaccc tgaaaccttg aaaccctgaa
       61 acccaaaaaa gcgtcccgtg gttaaatgat cgatcccgat agcgatcccg atagcgatcc
      121 cgatttggat gggcttttta ttcggaaccg ccgcgcctcc cggttgtcca gcgcaccagt
      181 ccccagagca gatcatggcg cgccataccg gacggcagtt cgacacgctc gtacagggtc
      241 cagaagggcg cccagttccg ttcgataccc ccgctgtggc ggatgaggtt gaactccaga
      301 gcacggaacc gcgaaccacg ggacgtcgtc tcccgcacga agaagggcca gacccgcgtg
      361 taccgctccg tttcgcgcaa gcccggggct ccgggcgagg gttgccagat tgtctcgcag
      421 gtgtagaacg gaaagaaacg cgtacgctcc gtaaggcgct tcggactctc ggtgcgggta
      481 tacttcagca acggccatgc cgcataacga cgccgctcat ccgacccctc gaactccccg
      541 tagaacggcc aataggaacg ttgaaacgag gcttcggtgg tacgggtttc ccagatttca
      601 cgcgtctcat aaaagggcca aggcaggcgc cagcgctcag ccgaatcggt ccgcgcatag
      661 gaaaagaacg gcggcagcac caatcgttgc gattcgcgcg cgcggttgac ctcgccgtag
      721 agaggaaaaa gcaggaacga ggagccgggg ttgcgttcgt cctcgtatcg cgcggaattc
      781 cagaacggcc acaaaacata ccggcggtcg gtcttctttt tggtgttgat ttgacggcac
      841 cagaacggaa agacgccctc ttcacgcaga tgcgggtttt cggtaaggcg ggagaagaag
      901 ggccaggggg tatagacgcg ttcgaccccg ttgacctcgt aattgaaata gagcggaaac
      961 agggtgaagt cgatgtcatc catcagcgcc atatgcggca gatggccgta cagggggaag
     1021 agtgcccaat agtcttcgcc cgcccgtgtg cgtccctcaa accacacggg cagaatcacg
     1081 gtcttccagg ccgcgtcgtc ccgggtgacg tcgtcatccg acccgtacgc cagcaacaac
     1141 cgccaccagg actgctggtg gtcgcgatga aaggtgccga gcggccacgc cacatccgtc
     1201 acgtgcgtgt ccgatacggg atcacgcacg tccgcgtaga aggggcgcac agcccagaag
     1261 acgccgcctt cacgcgtggc gcggtactcg aaaaacggcc ccagacgaaa acggctgacc
     1321 acctcgtccc tcgccgaccc ggtcagggat gcgggctccc cgaatgccgc aatctgttgg
     1381 agggcgacac cgcaaccgaa cgcgagcacg cgcgagaaac acaatacggc tttagtcgtt
     1441 ctggcggaaa tactcatgaa tgtgcttctc gcaaccaaag gtgacagcga tcagcgcggc
     1501 ccggatccac atcccgttcc gcatctggcg ccagtacatc gcgcggggat cgctgtccac
     1561 cgatttggaa atctcatcgc gccgcggcaa cgggtgcatc agaatggcgt gctgcggcag
     1621 tattttcaac tcgtcgagac cgaacttgaa ctgccgcgtg tcaatcttgt cactgtcccc
     1681 cttggccgca tcccactcgt cctgaatgcg ggtcatgtac accgcatccg cctgccggat
     1741 cgcggcgcga aaatcgtcag tcaactcgaa cgtcacatcc ttccctttga gcatggccaa
     1801 cacatcggga cagacctgca acgatggcgg tgccacaaaa atctgacgga tgttgggata
     1861 attcgtcagc agataggaga gcgaacgcac cgtgcgcccg cgcagcaggt cgccgcagaa
     1921 aaccacggtg cggtcgcgca acccgcccga ccgctcgaaa ctccgcaaca gcgtatagat
     1981 atccaacagc gcctgggtag ggtgctgatc ctgtcctgat ccggcattga tgatcgggat
     2041 cggccgctcg gagttggaga gcgcccacgc catccgttcg gccaacccct tctccggggt
     2101 gcgcatgatg atcaggtcga aatacgaact gaacgtccgc accgaatcct cgcgcgactc
     2161 gcctttgaac tccgaagaga tcgaggtgtc gcgcacctcg ccggtcggca cgccaagaat
     2221 ctgacacgcc gaaacaaagg agaggaaagt acgcgaactc ggctgcgaaa agtaaagcat
     2281 cgcgcgcttg tgcgaggcct gctcgcgtaa aaacccggtc ccttcacgcg ttttggcgat
     2341 actccggatc tcgttcgcca gcgtggcgag tttgtccaac agttcatacc cgaactgctg
     2401 ggcgaacagc gtgtggtacg gcatgccgtt ctcctgcatc aagtaggttg ccttctcaaa
     2461 gaacggtaac ggctcgaacg cttcccatgt gatgtcggac atgtttcgga tcatacaacc
     2521 ccctttcacc ggaccgccgc aaggcggcgc cctcccggaa cctcacaatg ccgaaagatc
     2581 agtcagcgcg gaaacataca gggccttgat ggtgtgcatc cggttctcgg cctcgtcgaa
     2641 caccttggag tacggggcct cgaacacctc gtcggtcacc tccaacgccc cgctttttgc
     2701 ggtcaccgta gtctcgtgat cgtggaaagc gggcagacag tgaaggaaga tcaacccgcc
     2761 gtcctcattt ccggtggccc gcacgagagc ccggttcacc tgatagggcc gcaacaggcg
     2821 cagacgtttt tccttctgcg cctcttcacc catggagacc catacgtcgg tatagagcac
     2881 attagcgccg gccacacccc gaacgggatc cgtgaccacc tcgacgccgg ccccgtgccg
     2941 acgggcgatc tctccggcct cccgtaccag atccggatcg ggcagcaggt cggagggtgt
     3001 gcagttgacg tagtggatgc ccgccttggc gcaaccgaac atcagcgaat ttgccacgtt
     3061 gttacggcca tcgcccacat acgcgacctt cagccccttc agccgcccga agttttcctg
     3121 aatcgtcatg agatccgcca gaatctgcgt cggatggaag acatctgtca gaccgttcca
     3181 gaccgggacg cccgcatagg ccgccagggt ctcgaccgtt tgctgggcgt acccgcggaa
     3241 caggatgccg tcaaagaggc gccccagcac acgtgcggta tccttgaccg actccttggc
     3301 accgaagtga agatcgtttt tcgtcaggta ttcggcggtc ccgccctcgt cacggatcgc
     3361 gatcgaggcg gagttgcggg ttcgggtcga actcttttcg aagatcaggg ctacatgacg
     3421 ccgactcagc aagtcgccgc gtatccccgc ccggcggcgc gccttgagcg ccaccgcgag
     3481 atcgatcaca ttcagcattt cctcatcact caaatccacc atacgcagca gcgagcgccc
     3541 gtacagttcc ggcatccagt tcaacatatt cgttctccaa tgggccgctc agtatacggc
     3601 tccatgccaa cgggtacaac ccgattgccg cgcccagtca aggggctatc ggcccggtcc
     3661 gcgccagtcc gaccacctcg gcggtccgca acggccggtc atagagttgc aacccactca
     3721 ctcgcccggc gatcgtggcc ggtccgggca catcaacagc cccaaaacct tccgggagtt
     3781 cggccgtccc ttccccgcca tctgtgccac cctccgggaa gcgcccctcg gccagcgccc
     3841 acatccgccc gcatgccgca tcacacacga aggcgacctg atgcgtcttc tctgcggtca
     3901 gcgcgccggg ggcggtcgtg cagacggccc tgcggttccc gtcgtatgcc tccagcctca
     3961 gcgagggcgc accccgcgat ctggccattg aaacccgcag accgcgacgt ccgttccacg
     4021 tgctgaacaa caccgcgccg ggctccgcgt ctgccaacgc gcaccacagc gcgacactcc
     4081 accccggccg gccgtgcgca ccgaatgcgg ctggcacgac cgtcggcccc gtaccgccgt
     4141 acgcaaaaac cagaccgttt gtgctgacgg aacgcgccat gggatcaggc cataacccgt
     4201 ctgttctcag gcggtccagc ggatatgcac gcacgaccct gccttgacgc accgcgagcc
     4261 aacactggtt atcctgttcc cagacggaac cgaggcacac ctccccgccc gccccgtcac
     4321 catacagcaa cagttccggc tcggaccagc gcgtcttggg atctccgatc tgccgcgcgc
     4381 ccgagcccca cacactcccg ccccgtccgc accgttcacc ggatctcccc cgccacaaca
     4441 acagcatgcg gtccccggaa aggaccgccg cgctgacatg cccggtaacg gccgcgaggg
     4501 tctgtccgga ttcgaaacgc agtttttcca gcggcatccc gttccctttt ccatccggat
     4561 tggcgcaccg caccggaccc tgcgcgctcc ccgcgatgcc gcacacggtt ttcccgtccg
     4621 gtgccgcaag acagtagaac gcgccctccc ccggatccgt catggcgcga aaccccggac
     4681 ccgtactttc cgtaacctca aagcgcaccc gtgcggcgtt acgttccgaa tacaggttcc
     4741 gcgaaaaggc gacccgtccc tcgatcgccc cgtccggctc gcggcgcatg acgagcggca
     4801 ggcagaccgc accgtccgcc agagaaacgg gcaccccgat tcccaccggc ataccccctc
     4861 cggtctgtcc tgcggcagcg gacgtcaagc gcagacgctc ggtcgaccaa gcaaccccgc
     4921 cgtcatccga aaagagaaag ccgtatcccc gcaccgcctc gtccgtccaa aagagataga
     4981 tacggccaaa cggcgtctgg tatccgatcg cggaaacctc ctctccgcct ttgaccgtca
     5041 caggcataac cggcgcagac catgtctttc cgcgatcacg actcacactg accgcaacat
     5101 cgcagaatcc gctgcgcggc ggcacaggct ccaccccctg caccgccagc caggatccat
     5161 cccgcaagac catgcagaaa ggcctgcccc gcgcccgctg gaacggcaac atcatactcc
     5221 cgtcgagtcg ccgcacgtcc gtgtaccgtt ccggccgcgg cgcggccacg gtatccgccg
     5281 catgacagag aaactgctcc ggggccaaaa cactgtcgga cacgcgccag cagtccagcc
     5341 agcccgagaa cgagttggag agggagggac gcccccccag caacagatca cgccacccgt
     5401 gggaacgatc aggcggggca gggttggcca cgcttccctg cgacacgccg tccagaaaca
     5461 actcccacac tcccaccccg ttccgaaggg gatcgtaggt cagagccaga tggcaccagt
     5521 tcgtgcacgc cgtcacatcg gcattcacaa aagaccgttc gaactgcaac cgtccgccct
     5581 gcgccacgtc actcacatgc aggtgaaacc gcactgcggc ggcgtcaggc cgcaacgaga
     5641 gcatccatcc gttggaatta tcgcgcgcgc cgaccacctg ccagaagcgt tcggttggat
     5701 tcgcggattt gcggaaccat ccctcgaccg tgaagcggtt ggtgagatca caccgcaggc
     5761 ccaaatccgg cgcgaccagc agactgcgct gtccgagcgc gccatccagc cgaacgcttc
     5821 cggtgttcgc gcgcgccggg cggctcctgc gccacaaacc gggctcagtc ggcagacgcc
     5881 cggacacctg ctccggcgca accgatatgc cgccgtcccg acccggcgcg agcgcatacg
     5941 gcccgccggt cagatcccgc aaatccggcg tcccgttcgg caaccggtcc ataggccagt
     6001 aggcgagcgt gcggggcact gcggcgcaca accatgcggc gggcggcagc acacccgcac
     6061 tcaccctcca gagatccatc tgtccgtaaa aggcgtcgtc cgcctcaccc gccccgccaa
     6121 ggaaaaaccc gcccggaaga tccgcgcacg ccgcccgtac gggattggtg gcaaccccca
     6181 gagacacacg gttcacgaaa aactcccaga ccccgcaccc cgcacccgtg ccgtcatagg
     6241 tcaggcacat atgccgccac acatacgaag cgtctttcgt tacgtcgcgg aaacggtgtc
     6301 ggagcgtcag gcgatccgcc gcggcatcgg cgtgcaagac gaaggccggc ccctgcgcat
     6361 cgcgacacag cgccacgcgc cacccggtgc cggggcggcc cgcgccgaaa agcggccacg
     6421 agacgccttc cgcaggatcg tccgcgcaac gcacccaccc ctcaaccgta aacgcattcg
     6481 tccacacgag ggcacggccg agtcccgcgg cacgcaactc cgccgcaggc acaccggaaa
     6541 acaacaccga accgcggttg cggccttcac cacgcgaagc ggcgggcaac gattcgtacg
     6601 ccggcagcac ggcgagcggc atgcggaacg aaggggttac cgcacacgat cccgtagcgg
     6661 ccagggaatg aagcccggca atatcgcgta aatccgcctc gctgcggaag gtatccaacg
     6721 gccagtacgc cagcgtccct tctccggccg caacacatgc ggccagcatc gtgatcccga
     6781 tcagccttgc cgtcccgctc attgcgtttt tcccaccgtt cacacctgta agctccaaaa
     6841 gaagagggtg cgggtacaga gtccccgcgt tcctaccgga tcagccgcaa cgccaactgc
     6901 gccgctgacg ccgcatccgg cgaataaccg tccgccccga tctcatcgca gaacgcctga
     6961 gtcaccggcg cgccaccgat gatcacccga acgtccaacc ccgatgaccg ggccgcctcc
     7021 accacttcgc gcatgtggac catcgtggtg gtcagtaagg ccgacacacc gatgatccgc
     7081 gccccatggg tccgcgcgag ttccacaaac gtcgccgcgg tgacgccgac cccggcatcc
     7141 accacacgga atcccgcgcc ctccagcatc atccccacca ggtttttccc gatgtcgtgc
     7201 agatcacccc ggatcgtgcc gatgaccgcc gtgcccaccg gctcgacgcc cgccgccaca
     7261 agatgcggtt tcagcacctc caaccccttc tgcatggcgc gggcggccaa cagcacctgc
     7321 ggcacaaaga cctccccctt gcggaaccgc tcccccaacg cgcccatcgc ctgacataag
     7381 ccgtcgttca gaatcaccga agcgtccaca ccctgttcca acgccagcgt cacactttgc
     7441 gtcacctcgg ccgctttgcc caaagccaac gcctctcgaa tcggttcgat gctcatccgc
     7501 tcacgcactc cggcggaaaa ccctccgccc gctcatcgtt tcaatcgcat ccaagaggcc
     7561 agcttcacgg ctcgcgcctc cgctgcctgc acgtcatcag gcgttacggg ccgggatgcc
     7621 ttttccagcg cttcgatgcg caacagcagc gcctgctggt tcccctggta ctgctcgcgc
     7681 tccgcctccc ggctcttcct ctcgtccgcc aattcctgct cccggttctg caacacctcc
     7741 tccatctcct gaaggcgtcg cccgagtttg tctcctactg ccgcccgttc ccgccatttc
     7801 cccgcttcgg cctccatcac gcgtatcttg cgctgcaaat cccgctcgcg ttccgtcgcc
     7861 aaccgcacct cacgctcata cgcgaccttc atgttgtcga actccgtccg caaccggttc
     7921 gcattcggat cggaaggttg cccgcgcaac gcgcggcgcg tcatgtcggc gggcgtcaca
     7981 cccaaccgtt gcagtagcgt ctgcctgagt tcggacagaa actgctgccg ctgtgtgctc
     8041 catgttttca gttgttcgag atgggtacgc tccgactcca gtctctccgc cagcgcgtcg
     8101 gcctcgctct tcatgagttc aaagagcgcg gcctgatcct cgtaaaaccg cgtcgtctct
     8161 tccggcgaca acgcgcacgc ttgggtcagg gtggcaatct gctcctgata agcgttatcc
     8221 cggttgttgg cgtccgtgag caactcggca aaggcgacct cctgttcgcg cagcgcagcc
     8281 tcgagcgtct cccgctcccc tttcagcacg gtcagccgtt cctttgcctc cgaggcttgc
     8341 gcctccgcct ccgcgagccg cgttcgcagg gtttcgcact cctcagtcgc cccgcgcaga
     8401 cgttcgcatg tgacggagag cgccgccaat tcttcccgca ccgtcaccaa ctccttttcc
     8461 gccgccgcgc acttcgcctg cgtctccgct tcggcgcgct gcaaagcctg cgcctgcttg
     8521 gctgcggcgt cggagagcag caagcgtccg tctgcttctt cgcagcgttg ctccagcttg
     8581 cggatctgct gtttcagccc ctcgattttc cgctcgttcg cacgcgcgtc cttctcggca
     8641 ttccgcaaca gcgagtcgcg ctgctcggtc acctcctgaa cctgtccggc cagtgtgccg
     8701 cactcgcgcc gcaacgtttc cagcgcatcg ctttccgaaa gtcgggccag acgttcgcgc
     8761 aattcggcga cctgcgcctc cagttcccgg ttgcgctcca gcacctccgc atccggcgcg
     8821 gcagaccgca acggcccggg acgaggcgca gacgatagat ccgcagacgc gtcggcctcg
     8881 agctcctccg tggagaccag caccgaccct tccggcacct tcccgctctt cagcagcgcc
     8941 tctgccgcgc accggttcag cggcccccgc agttcgccca tgccgtcatc cacaaaccag
     9001 cacatatcaa gcagcggctc cgaaaccgcc tgaacccact tcttccggtc cgtggagatc
     9061 tcatgccccg gtaaaatccg tccctgttcc gcccagacaa tcaggccttc cgtggaaacc
     9121 ggtccgaaaa ccgtctcgcc gttgatccgc aggaaccact gacgcgtatc ctctttgccg
     9181 ttttgcgtgt ctgccataaa aactctgccg taccgtatcc gaaccccgat tcccgccgcc
     9241 cctcaggcgc acccgcgccc cgggtcactt ttttttgaaa aaggatttac cccgctcgcc
     9301 cagccgctcc agttcccgac gcgcctgccg ttccagttcg gccagcgaca agccgggttt
     9361 cgcctgcgag gccacccgga tcacaggggg cttgccgtgc gaaggacgtt tttgctccgg
     9421 cgcgagcacc tccggctcaa tcacctgtgt ttcgcgcggc acctctcggg agttgcgcgt
     9481 tcccgcctcc gttttgcgaa cggcaggctc atcaaccggc tccgcctcca gccgcacagc
     9541 gtccccttcc gaaggaacgg aatcgagcgc ctccgccgcc tgccgcgcca gcgcgcgcgc
     9601 ggcctcgaca cgccgacgcg tatcggccgc ctcgctccgc aatgcgtccg tttcactctg
     9661 cagcgcgtcc agacggcttt gcctttcaga gagttgcgcc gctgcttccg tcacctcccg
     9721 ccgcaggcca gccgcctctt cttccatcgc ctgcacgcgc cggtgcacgt tctcgttctg
     9781 gtccgcctcg cgccgcgcgt cagccaactc cccttccagc gaggcgatcc gcgtcgcctg
     9841 ttccgccaga ctgcgttccg cgcacttcac cgcttcccgc agttgaccgc gttcttcctc
     9901 aaaacgttgc gcctgatccg cagccgccgc aaccgccgca gcctcccggt cccgcagtgc
     9961 cttgacctcc tccccggctt gggtgaggcg ctgacgcaac gactctcctt cgatttccca
    10021 cgcctttctt tccgcccgca accgtgcggc ctgctcctcc agaccgcgcg cgcgttcccg
    10081 ctccgcatcc cgctcccggg tcaacgcggc gcgcgcctcc tcctcaagac gggcccggtc
    10141 cgccgcatcg agaggctgac gcgtgaaaaa agccgtctca cccgacaacg acccctcttg
    10201 gaccagatcc cgcatggcct gtccgtgaat gggtccgtaa aaggctcccg gcgtaacctc
    10261 cgccacccag tccatcccca gttccgcccg ggtcgtcacg cgctgccaca ccgtcccgtt
    10321 ttcggagatc tcgctggtgg gtgccaaacg gccgtccgca gcccacgtct gaagcgtctg
    10381 gaaatccaca gggccgaact gttcgccctg ctcgttgcga acacgccaga ccgctttttc
    10441 accgcccttc cttttcccca cgtttgccac acgctgcctt tccgctgaaa accgctgaaa
    10501 aaaccgttcc ttatgataaa ggaagccggt cggcttcaca atcccttttc cgcacggttc
    10561 cgcccctcga accccatgcg gcccggtcgc cgcaatggtc cgggtcttgt ccgaaaccgc
    10621 ggcatggtgt atagttctgt tatgaacgct tgctcttcga caccttttct gaaacggccc
    10681 accgcttgga tggccgcgcc gttgctctgg atcgcgggtg ttgtcgtgct ttcggtgatc
    10741 ggtttgaccg ccacgcccga ctggatcgcg aatcacttcg atcaggatgg ggcctcgccg
    10801 gtggaggccg cgacgatcgc gctgttctgg ctccagatcg cctttttctg gctggtgccg
    10861 ccaatgcggc ccggacgctg gcgcgccttc tggctgacga atttttcgct gttgactttc
    10921 ttcgccatct gccgccagct cgactggcac aggctcctcg tcacaccttc agggctcccc
    10981 ggcaccacgc gcggcacacc gtttaagatg aagtttttgc tgaacgcccg caacccgctc
    11041 ccggaccgga ttctggtcgc gctctgcttc gtgctggtca tcgcgctgtg cggcggcacg
    11101 ctgctctatt ttctgccgcg gctgctccga ggcctcttcc ggatgcaccc ggtgtgctgg
    11161 acgatcggat tcttcggcgg cacgctgatc ctgatccaga ttttcgaccg gatgagttcg
    11221 gtgctgcgca aggatttcgg catccacctt acggatcgcc agcatgcgct gaccaccgct
    11281 ctggaggagg ggctggagct gctgctgccg ctcttcatta tcctcgcggt gctccaagcg
    11341 cacttcatct atctcgattc tgacgcggaa agcgggccgc tggcccgcca ccgtgcactg
    11401 taaccccctt tcgcgccgca cggcgcggaa agtctgaccc atgcgaatac tggtgatcga
    11461 agacgacgac aagatccagt ccttcatcgg ccggggattg agtcaggaag gccataccgt
    11521 ggacatggcc tcgaccggta ccgaggggct gcgtctctgg cagtgtacgc aatatgacgc
    11581 ggtcgtgctc gacatcatgc tgccggaacg gaacggaatc gatttgctcc ggcaaatgcg
    11641 ccacaaaggc gacccgaccc cggtattggt gctcagcgcc aaaagcgccg tggaggaccg
    11701 cgtgaccggt ctggaaatcg gtgccgacga ttatatgacc aaaccctttt cgttttcgga
    11761 gctgatcgcg cgcctgcacg cgatcacccg gcgcatgcag agcccggcgc aatccggtaa
    11821 agacgccact tcgctcagcc tcggcggggt gactctcgat ctgttacggc gcaccgtcgt
    11881 gcgcgagaat cgcaagatcg aactgcagcc gcgcgagttc gccctccttg aactgctgat
    11941 gcgtaacgcc aaccgccccc tcaacaagac ggtgatcctc gaacggatct gggattacag
    12001 ctttgatccg caaaccaacg ttgtggatgt tctggtctgc cggctgcgca cgaaactgga
    12061 tgccgggttc agcgtgaagc ggatccagac catgaggggg atcggctatg ttttccgcac
    12121 gacggaataa ccgcctccgt tccttcgcga tgtatctcgg catgggctac ctgatcgtct
    12181 atgccctctg taccggcgtg atctttttcc tgatcacccg ggtcatcacc gtctccgccc
    12241 gcggctttga ccgcgaggat gtcctggccg aaagcgagga actcgtcgaa ctcctcaccc
    12301 agaacaccga cggcaacctg ctggccgaac aggtgaccat gggacggtat ccgccctcca
    12361 ccatcttcat cgtgcgcgtg atcaaccgcc gcgggctggt tgaatacacc atcacctggc
    12421 cgaagaagat cgaccttccg gattggcgtg ccttcgcgga aggcgacgaa aatctgcccc
    12481 gcgagggatt gagcgaatac tacatccggc ccttgaaacg gcatgtccag atccagacca
    12541 cccatctccc ggacggccgt gtgcttcagg tgggcaaggg ttcgtttctg gaggtggacc
    12601 agaaaagcat gctggtgcgc atcctcctga tctatgcggc cctctccacc ttcttctcga
    12661 tcgcctcggg catcttcatg atgctgatca ccctgcgccc catccaccgc atcacaaaat
    12721 cgatgtccga catcatccgt tcgcaggcct ttgagaccgg tgcgccgccg gtccgaagcc
    12781 tgatcgcgga actcgacacg ctagggcagc ttttcacgat catgaccggg aaatacgcca
    12841 acctcatccg ggccatgcgc gagaccatgg agaacgtggc gcatgacttc cgcacgccgc
    12901 tcgcgcgtat ccgcggcgca tcggagctgg cgctgcaccg cgaacacctt cccgccgaac
    12961 tgaccgagac gctcgcggac atcatcgagg attgtgaccg cctccagctt cagctccaga
    13021 acctgatgga cgcgcgcgaa atggagagcg ggttcgtccg gctcaaccgc cagcccttcc
    13081 gcctcaacca actcctcggc gaaatcgtcg acctctactc gttgcttgcg gaggagaaac
    13141 gcctgaccct ctcgtacgtc aatgcctgcg accgcgatgt gctgttcgac ggggaccgga
    13201 accgtctcgc ccgcgccttc gccaacctca tggacaacgc cttgaaatat accccgaacg
    13261 gcggccgcgt ggacctcacg ctggaaacag gccccgacgc cgtgcgcgtg tgtgtccgcg
    13321 acacgggcat cggcatctcc gaagccgaac agaccctcgt ctggcaacgc ctgttccgcg
    13381 gcaaacaggc gcgtgaaacc gaaaaggggc tggggctcgg cctgaacatc gtccagatca
    13441 tcatttcggc gcatggcggc acggtcaccc tggaaagccg acccggcgcg ggcagcgttt
    13501 tccgcgtgac gttgccgttc cgcgatgcgc caagctgaac ttctgcgttg agataagcca
    13561 gggtttccga cagcttcccg taaccggcaa cccaaaattc tcatccccgt gccacggtca
    13621 gagcccacac cgtgcgcgca ccggcccttt ttagtgcatg cgcacaggcg tccaacgtgg
    13681 caccggtggt catgacatca tcgaccagca gcacacagcg ctgcgtcacc catgccggcc
    13741 ggaccacctc aaacgcgccc cgcatgttga ggcgccgacg gaccgcgtcc agatgcgtct
    13801 gcgtctccgt atggcgcatg cgtcgtaacg cgcggtcatc gtagcggcgt ccgatccgct
    13861 ccgccaaggc ccgcgccagc agcgccgcct gattgtagga gcgctcgcgc tcgcgaaccg
    13921 gatgcagcgg caccggcacc accacatcca cggcaacggg atcgaaatgc gctgtcaggc
    13981 atccggccaa aaaatccgtg aggtcctggc acagccaaag ggcctttccg tatttgaagc
    14041 cgtggatcat ctcccgtaag gtgccgcgga agcgtccggc cgagcgcgcg cggtcgaaat
    14101 gcggccgcac ctcccggcat acgccgcatt cgaacgcatg gcccacacgt ccttccgcat
    14161 gggcgccgca cctttcgcac aggctctccc ggtaaagctc gacggtgcgg aaacactccc
    14221 agcacaacga ccggccgggt cggtccgacg gcttgccgca ggacgagttg gggcagaccc
    14281 gcggatagag ccactccgcg agcagaccgg cgagttggcg ccctttcccg gtcatgtgcg
    14341 cctccgtgtt accagagttt atcgggatac acgccatcgc ggacccgtgc ctgcaccgca
    14401 gccatcaccg caggcctatc ctcccgatag gtcaccccga accaggcgct ttccgtcggc
    14461 agcacctcga cctccgccgc accctcgcgg atcatcccgt ccaccacaaa cggaatgtac
    14521 cattcggctt tcggatccgc cgcatgcgcc gccagccaag cggggaaacg ttcttccaac
    14581 gccgcgaaca aagcgggcgt gaagccccac aggttcatcg acacccgctc cgtaccgttc
    14641 agccggaccg gacgcgcggg atcctcctga ttttcgacgc cgtccgccac acgcgtcaaa
    14701 cgcgtcatct ccgtgactcc cgccagcatt ccctccggcg tcacgcggca gatgccgcgc
    14761 gccacgccgc catgttccga gagcgtctga tccaaacgat aaccgaccat agcgaagcgt
    14821 ggttttgggg cgggccgcga tgcggagaga aacgccccca gccgcgcgaa cgcatcctga
    14881 ccatagaagt catccgcatt gatcgcggca aacggtgcct cgaccacctg ccgcgccgaa
    14941 cggatcgcgt gcgccgtgcc ccacggcttg gtccgcccgg ccggcaccgt gaaccctgcg
    15001 ggcagatcgt ccaaagcctg aaacgcataa tccacctcga ccgccccggc gtatttcgcg
    15061 ccgatggtct cgcggaaggc cttctcgaaa tcccgccgga tcacgaacac cacgcggttg
    15121 aatccggcgc gcacggcatc gaacaccgaa tagtccatga tcgtttcgcc ggacggcccc
    15181 accggctcga cctgcttcaa cccgccgtac cggctgccca tacccgccgc cagaatcacc
    15241 aatgtcggct tcatgtccgc tcctgtttcc caacgtctcc agtaaaaccc gcgataccct
    15301 tcccgtgcgg gcggagcccg cgccccaaat cagcctcaca aaaagaacac aagcgcgttc
    15361 ttttcggcgg tccgagacgt cccgccatac ctatgcgatg ccattggact ttcaaccacc
    15421 cggaccgttg ctctcgccga tgcagcccag accgaacagc gtcagttgcg gatcaaggac
    15481 gaactccatc ccgctctcct gcaacgccca tccggcatac ccgcccgtgg cgaccagttt
    15541 gaagtcacac gccatcgttt gccgcaagtg acccacgatc tcgcgcacga tgccacggta
    15601 gccgatctgc gcaccgactc gcatcgcctg cagcgtgctg cgcccgatcc ccggacaggg
    15661 cccctccaaa ttgacgcgcg gcaacaacgc cgtgcgctcg tacagatagt cggtcatcag
    15721 cggcagcccc ggggtgatca cgcctcccac ataacgccgg tccggggtca ccacatcaaa
    15781 ggtcagcgcc gtgccgaaat ccgcgacgat cagcggcgcg ccgtaacgcc gcacgccgcc
    15841 gcacgcaccc gccagccggt ccgcgccgat cgtttccgga gcgggataat cgatcgtcac
    15901 ttccatcggt atcccggccc ggaccaccag cggcgcgcaa cccgtttcgt tgcgtacgag
    15961 tcgacgccac gccgcattgg cccgcggcac aacggatccg agaaccgcac cgtggatccc
    16021 gccgcggatc gccttgcgca ctgccgcggc acacgccgca gggcgcttca caatcccacc
    16081 ccgcacatgc gtgacacgcg agatcacacc ccggtcatac agaccgatgc ctgtcgaggt
    16141 gttcccgaca tccatgacga gcaatcgcac gactcagtcc caactccatt ccagtgacag
    16201 atccacagca ggcgccgaat gcgtcaggca cccgagcgag atcgcgtcga cccccgtcgc
    16261 cgccacctcg cgcgcacgat ccagcgtgat cccgcccgag gcctcggtct tcgccttgcc
    16321 gcggcacagg gccacgcatg tgcgcatcag gccgcaatcc atgttatcga gcataatcca
    16381 ttcgggtttc ccccgcaacg cgctgacaca ctcggcctca ctctcgactt ccacctccac
    16441 tgcgagtgcg ggataagctt tccgcgcagc cgccaccgcc aaatccaacc ggtccggatc
    16501 gccccccagc catagccgcc ggtggttgtc tttgatcagc acccggtcgt acaggccgaa
    16561 gcggtggttg acgccgccgc cgcaggtaac cgcgtacttt tcgaacaacc gcaggttggg
    16621 tgtggtttta cgcgtatcaa ggatcagcgt gccgtacggt tttaccgcct cgacgaatcg
    16681 ggcggtcagc gtggcgatgc cgcacagacg ctgcatgaaa ttcagcgcgg tccgctctgc
    16741 ggtcagaatg gaggcggccc ggccgcgtag ggtcatcagc acatcccctg cggccgcggc
    16801 atgcccgtcg ggtcgaagcg tctcgatccg cagcgacgca tccacgcaaa gaaaggccgt
    16861 ctgtcccaca ccaaccccgg ccacgcggca tggctcgcgc gtgagcagca cggcggtcgc
    16921 ctcgatctgc gggtcgacca gcgcccgggt cgtggcatca ccggtgtcaa tatcctcctc
    16981 cagagccgcg cgcaccactg cgacagccct cgggtcaccc atcacaccgt caccctccat
    17041 acggcacccc tcctctccaa tcccccgcct ccgtgcgcag cgcgcatccg cagagatacc
    17101 cggcgatccc gcccgccagg tgtgcggcgt aggccacgcg ggactgcacg aaaaccgcct
    17161 ccgccacagt gacggcacct aaaaaccacg ccagggtccg cgccttcagc cgcaacggga
    17221 aaaagagcaa cagcacatat acttcacgcc gtgggaacag ggcgctgtac gcgccgatga
    17281 gcgcaaacac tccgcccgag gcaccgatgc acagggctac gtccagcgtt tgcccggatg
    17341 acgcgcccgc ctgaagccac gcggccgcgg ttcgcggcag ccattgcgtc agcgcctcag
    17401 cagacggcaa caggggggtc agcgccgtca ccatcagcca cccgagccca cccaacaatc
    17461 cgccggtcag gaaaatccat ttgaaacgcc gcccgccgat ctcggcttca agcccggagc
    17521 cgagcagcaa caccgtcagc atattcattg caaggtgttg ccatgaggcg tgcaggaaca
    17581 tgtaggtcac aagctgccag acaaacccct cgcgcaacag gggccagctc aatccgaaac
    17641 agtgcagcaa cgcctgatcg tacccgtacc ggtatccgta taccgcgaca aaatcaaccc
    17701 gcgccgctgt ctgctggatg agaaagacac caaggcacac ggccaccagg ccgattgccg
    17761 caggcgctga tcgcgcgaaa cccctcagac gccagactcc tctgcgcatc cgattgatca
    17821 acccggcctc cgatccggcc tcaggcggat ttgatcgcgt tccagttgac gtacagcagc
    17881 gcggtgatga tcagcagcac aacggtcgcg aggatcgcgc acacgctgcc gaacatctcc
    17941 tgtccggctg cctgacgcgc ggcaacctcc gccaccggat tgcggtgacg cgcggcaatg
    18001 aacgcacccc ccgtcgcggg cttggtctct tcaaccgcct cgatagacga tttcttcaat
    18061 ttcatggtta cgattctatc agaacgggcc cccgttgagt agcgcatttt taaagaaccc
    18121 cacaaccctg acttgcatgg gggaatgcac acagacgcga aaaccggaga gcctcacctc
    18181 agcgacaccc cttttttctc cagcagcatc tcaaacagcg cgcgcccctc cggggtcaat
    18241 tcccccttgc aaaccaacgg caccgcgccc tcagccgttt ttgcggctgt aaacagatcc
    18301 acggtctcca ccccatacgc atcggcaacc ctcatcacac tctcagcata gctccgcgcc
    18361 gcggccgcat gctcgcaggg tttcccgccc ggcacgcaac cgcacccggg caacacgtca
    18421 aacgccggcg gcacggtcag cagcacgcgg ggcgaaccgc atgccggccc cgaaagcaga
    18481 ccggtcatcg ccgcgaggcg tcgctcgaac ccttccgttc caccctcccg gctgatggcc
    18541 agcagcgaag gcgcgaccac caccgtgtcc gcaggcaaaa ccgagcggag cacggtgaag
    18601 ggctgtagca acgtcatccc cgtagccgtc tcactttgct ccacggcctg catatccacc
    18661 acccggaagc ggatttctgg tttctcgttc cccggttcgg cgttcagcgt tcggcattcg
    18721 acgttctgcg ttcccccata gaggaacccg tccagcacag ccaccccgtt ggtcccgatc
    18781 tgcggcacgg acagcggttc cgtgtcacgg gccgtgcggg aggccaccag caccacgaaa
    18841 tccggtccga ctttcaacaa cccacccgaa accacatccg gcaacaccgc aaccggttcg
    18901 cgaagaaaac gcaggcaccc ccgcccgatc tcaaccccgg catggcgcaa ggaccagatg
    18961 acgcgcgcca cctcccccac ccggaaagtc gtgaggtaca cgcgtgccca accgcgttcg
    19021 gtgaccagcg tgtacgtggt ctcctcgcgc tcccccccgc gtccgcccga tcatccggat
    19081 ccgttcctcg gcgttggacg gcatggccta c
//
LOCUS       DAOSRB010000030        19039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3047_length_19039_cov_7.018911, whole genome shotgun sequence.
ACCESSION   DAOSRB010000030 DAOSRB010000000
VERSION     DAOSRB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19039
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3047_length_19039_cov_7.018911"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLZ74_02925"
     CDS             <1..638
                     /locus_tag="PLZ74_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOR97305.1"
                     /translation="VIREYGADSMRLYEMFMGPLEAVKPWNTKGVEGVFRFLKRAYRM
                     VTEQPITEDPLQPEQLRMLHATVKKVTDDLEDMGFNTAISQLMIFLNAFSGADKPLPR
                     LAAETFVLLLSPFAPHLGEELWQHLGHEKSLAYAPWPVYDESLLQLDEVEILVQITGK
                     PKARVMMPADADEETMREMALSQSVIREALTGKTVRKVICVPGRLVNIVAT"
     gene            830..1528
                     /locus_tag="PLZ74_02930"
     CDS             830..1528
                     /locus_tag="PLZ74_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOR97306.1"
                     /translation="MIPYFYDPRLILVMLPALILAGIASVLVKTTFNRYARVRAYSGM
                     TGAQAAERMLRANGVFDVTVEETGGFLSDHYDPTRKVLRLSPDVYRGNSLSAVGVACH
                     EAGHALQHANSYGPLMLRTALVPVTSISSSLAFYVVAAGFLFRPLLLVGCAMFAVAFL
                     FSLVTLPVEWNASARAKQHLVTSGIVSPGQEAEAGRVLNAAFLTYLAAAVSSLITLLY
                     YLARAGVIGGGRRD"
     gene            1551..3431
                     /gene="htpG"
                     /locus_tag="PLZ74_02935"
     CDS             1551..3431
                     /gene="htpG"
                     /locus_tag="PLZ74_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOR97307.1"
                     /translation="MSKKTHVFKAEIQQVLDLVIHSLYSKKEIFLRELISNASDAIDR
                     AQYLGLTDPTLIADAPAWRIDILANREERSLTIADNGIGMSEEELDKNLGVIASSGTK
                     AFIKALREKESVDIPELIGQFGVGFYASFMVAEQVTVITLKRGADQKAVKWSSTGDGG
                     YTLEDAERDQPGTTVVLKLREDMKEYLDEWRIRELVKTYSNFIAYPIRFSDKGEMPAE
                     DSEPLNTIKALWRRPKSEIKEEEYNEFYTHLSHDHRPPLKTIHFAVEGVTEFRALLFL
                     PKEAGMDLLLPHKQRGLSLYVRNVFIGSDFDLLLPDYLRFVKGVVDSSDLPLNVSREM
                     LQDDALIRKIKSNVTGKILATLTEMMRDEPEAYSTFYTSFGRILKEGLHWDWENSEKL
                     KSLLMFRSAKQENNGFLSLKAYVESMPESQKDIYYLLSDDLATARQSPHIEALLQHGY
                     DVLFFTDAIDQWVVERLREFDGKKLVAVDKGTLELGTEAEKAETKKRIETADAEFREL
                     SAFLKEHLKEKVGEVKLSTRLTDSACCLVADEHALNASMERLMRAMNQEVPKQLRTLE
                     INPDHPVLKRMRQMLETDKSDPKLADYADLLLGQALLSEGSALPDTQRFTRLVSELMS
                     AS"
     gene            complement(3415..5229)
                     /locus_tag="PLZ74_02940"
     CDS             complement(3415..5229)
                     /locus_tag="PLZ74_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOR97308.1"
                     /translation="MKNRCDAADGEEGQQVFCHLWRLRDPGGGSWSGELAESALATAL
                     AAAALADAGEADDRVQADSGLNWLACHVNPDGGWGDTPHSRSNLSTTLLVHAALQRFA
                     GERHAKTVARAGEWIDRQLGEARSPASVARALRRTYGEDRTFAVPIQAFLAMCQDTRQ
                     AWQWVMPLPFALACLPQRLYRLLRLQVVSYALPALIAVGLCRHLRVARNRRIAAWGRV
                     LAPALLRKLAALQPDHGGYLDAIPLTAFVVLALHHAGFGGHAVVGKGLAFLRHSARAG
                     GGWAIDSNLRTWVTSLAARAITAYREGAGAGRVEAEALTPIARWLIQVQKTERHPFTG
                     AAPGGWAWTDLPGGVPDADDTAGALVALKRLRDAGCAVDFRAAVRDGVRWLLDLQNRD
                     GGLPTFCRGWGRLPFDRSCPDISAHALAAWAAWRNDPALPSYAERKVDSAIRRVLGYL
                     KQEQQPDGSWIPLWFGHQDAEHGQNPVVGTARVTDALRAAHYAADAVGWAGEAEAMRT
                     AGERWLIRSQKPDGGWSAGKTATVEETALAVIALLGGDAPCRAAAERGRNRLKEAWRS
                     GNVPQATPIGLYFSVLWYHEQLYPIVSSLEAFAVRTPT"
     gene            5472..>6910
                     /gene="rpoN"
                     /locus_tag="PLZ74_02945"
     CDS             5472..>6910
                     /gene="rpoN"
                     /locus_tag="PLZ74_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOR97309.1"
                     /translation="MAVPSLSLSQQQRQVMTLAPQLRQSLEMLQMPVMELRAAILNEM
                     SVNPTIEEVEDPSEVSLATVTAPEPQQQSAADEELDFNPDIDAILRQDDEWRDYFMQG
                     MENAPSYEEENEKRQYLLDSIRQKASLQDYLLEQLTLTDLQGEDYDLAVLLIGNISDD
                     GYFTGSLPDIIMVSGRSEREVLDVLKVIQTFDPPGVGARTLRECLLLQLALVEDSPWE
                     DEARLVIDKHLERLGAHDEKYLCKVLELTREELAHVTALIRSLNPRPGRAYVQEKTEY
                     VEPEVFLVREKGRLVARVDNQRLPHIHISKHYRSLLEDRNTPAETKTYIRERIRAGAF
                     LIRSLYQRQETIRKIAQEIVDAQADFWEKGVMALRPLTMAEVARKVGVHETTVSRTVS
                     NKYMRTPRGVFEMKYFFTHGLKSEDGVSVSNKSVQDQIRRLVEAEDPTAPLSDQALEE
                     KLSEQGIKVARRTIAKYRGILKIPPSHKRR"
     assembly_gap    6911..7010
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7095..8105)
                     /locus_tag="PLZ74_02950"
     CDS             complement(7095..8105)
                     /locus_tag="PLZ74_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR97310.1"
                     /translation="MAFGEILKNARLQRGLTPSDVAESTHLLVQVVEDLEREQFSRVA
                     APIYGRGFIKLYAELLELDPEPLIREFMDLYTGARVPAIRTRDVPHEAEQDAAETASA
                     VTPRQAPEAGPQRQRIEPRPAVMVRPVKEAPESAAPPAEDADRVAVKSRTEETGAERE
                     VRSARHGAHDLFGWVVRPEEEEKPERDVPQEPDLFHPAPSVTPRQPDAADAEKRKSAD
                     RFAVAQRRVLPIFKIGGRANDRAPGGFADEDSDAHARRNARIRAFKEGFMKLRTDVGR
                     RLPVTLPHKSVLMLAGAGVVVLICLLAGISLLFRLTGSGVSEGPAPVFETVSPPPDLY
                     ID"
     gene            complement(8124..10619)
                     /locus_tag="PLZ74_02955"
     CDS             complement(8124..10619)
                     /locus_tag="PLZ74_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOR97311.1"
                     /translation="MSERLIEQQDRMELERRIQRRWTGVLLFPIALFPLLSLLTYNWR
                     DISWLQAPPLTPPSNLIGIVGAWSVFIGYSLVGLALWLVPVMVLLFSVMLIYGRAVRL
                     VRRAFWMLIFLIALCCLLQLGSATVFDPVLERLNLKPNAGGAVGYVLMTCLLARWFSP
                     VGGGILMLSIMLCSLFLAVGLRNIVAGLGWVTGWWTTRPRAADKTEPPGTAEKEDSEA
                     DDGLTRRERERQQRQAIREAARRAKEEARQAREQERQREPAPAAPAPAPRVEPQQVVP
                     EKAAGTRTAPAVVAQPEPAATLPAAYELPSVRLLNPIPKGAADHGNITETSDILVETL
                     SQFNVPVEVVGTTPGPVVTQYELRPAPNIKVERIANLTANLQMALEATSLRVQAPIPG
                     KNAVGIEIPNRRARAITFREIIEGEAWTRNTCEVPLLLGKDVAGDDVVFDLTKAPHLL
                     VAGATGSGKSVCLNAILTGFLMSRTPDQLRLILVDPKRVEFTCYNELPHLLVPVINDP
                     KKVAFGLRWAIMEMEKRYRLLQKEGCRNIVAYNSRVIARQDDLFGEEDALSPQASAVD
                     ETPDRLPYIVIVVDEVADIMASVGKEVEPAIARLTSLSRAVGIHLILATQRPSVDVIT
                     GTIKSNIPGRIAFKVSQSNDSRTILDSPGAEELIGKGDMLFLREGGQLTRLQGAWVSD
                     EEIARVVAYVKEQSAPCYDAALSGRLEKIRESEPGAALSDEEDGEGGSEQGMEDGESD
                     DVLLPNAIEVIRMTRRASTTMLQRRLRIGYTRAARLIDILEERGVVGPHAGSNSREIL
                     IDLDKEIPVGGGTDRAGAPLPDEEAEADESPRV"
     gene            complement(10625..11638)
                     /locus_tag="PLZ74_02960"
     CDS             complement(10625..11638)
                     /locus_tag="PLZ74_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97312.1"
                     /translation="MQRDKVLSILRIVFLNNGYWKLLSLVIAVLVYFSIRAEISHVRV
                     ISIPVEAEFDAAGAGAAIESVEPRSVQVTLRGSYTDVNQLSLSVARCVVRPRQKKGNL
                     VDSVPVKIRRGNLRGVRGIRVVKVDPNVAVVKFDVPMSLPLMIARPAEIGTARGRVQL
                     VYDLTNAVVRGSRRQLSTLNPEVTQIQTEPINVDGRSQTFSTRVRLLPPGDVVNATVE
                     PPEMVVQVQILSEKATAKLERVPVVVYPHDMVQRWHVEPECVEVELSGRGEIVKAVTP
                     EQVTAVVSGNLPLSRETNGVPVMIHLQQGLEVSEVRALPPTVRLIPVTASVVGADTPA
                     GQK"
     gene            complement(11625..12488)
                     /gene="cdaA"
                     /locus_tag="PLZ74_02965"
     CDS             complement(11625..12488)
                     /gene="cdaA"
                     /locus_tag="PLZ74_02965"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOR97313.1"
                     /translation="MFDGVQIGFNDVMQILILYLGIYALIRFVKGTRSAQVLLGFCVL
                     VVSLLLFTYLFRFDVLAWVVYFFLIYLALSMVVVFQQEIRRVLALVGGQRLFSRQYTR
                     QQSSVPELICQSVMNLSRARIGALIAVERGISLCGYEETGVKLNALFSHELMVSLFTP
                     PLPLHDGGIILRDGRVASAHCLFPVSNQGELAASGMRHRAAVGLSEETDAVVIVVSEE
                     TGRISAAYNGRLHQYEDDKIGKMILRWLRVAMPQQTGDASTVTEWLTLKLEKWLKPLA
                     MSVGGDDHAKG"
     gene            complement(12481..13350)
                     /gene="folP"
                     /locus_tag="PLZ74_02970"
     CDS             complement(12481..13350)
                     /gene="folP"
                     /locus_tag="PLZ74_02970"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOR97314.1"
                     /translation="MERDSGRTWVCRGCVVPLRHTLVMGIVNVTPDSFSDGGKWFDEE
                     RAVAHALDLVEEGADILDIGGESTRPGAEEVSTEEETRRVAPVIRMLAERSGVPVTVD
                     TRHAAVARAALEAGACAVNAVQPFTEDAEMVRLVRDSGAGVVLMHMRGTPRTMSDLTE
                     YRDVVGEVEAMLRDALACAVESGIGRERILLDPGIGFAKDTAHSVALLAATARLSRLG
                     PLLVGASRKRFVGEVCGEPDARERVGGSVGAAVWCALHGAAVVRVHDVKATRQALAMV
                     RRLAEATVGETHV"
     gene            complement(13380..15413)
                     /gene="ftsH"
                     /locus_tag="PLZ74_02975"
     CDS             complement(13380..15413)
                     /gene="ftsH"
                     /locus_tag="PLZ74_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOR97315.1"
                     /translation="MSKESDHKRELPNQPPALPNRGGKPEGGATSGALPPKRVFGAWM
                     VVGLLLLFMLGMFYYKPEGAMRPMDQLEFRGLVEEGLIEKVELVREANGMTYLRGVRK
                     GAAKERFRVNLIPSENLEQFLLEKKIPCPLVYNTNVFGALLRDILPVILILVILYFVF
                     IRQIKSANMGAMSFGKSRAKMLSQDKKKLTFANVAGIREAKEEVQEIVEFLKDPRKFQ
                     VLGGRIPKGVLLMGPPGTGKTLLAKAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDM
                     FEQGKKHAPCLIFIDEIDAVGRSRFSGIGGGHDEREQTLNALLVEMDGFETSEGVIII
                     AATNRADVLDPALLRPGRFDRQIIIDLPTLKGREEILRMHAEKIKLAHDADLSRIARG
                     TPGFSGADLANLLNEAALLAARANKRAVGQSDLEEARDKVLWGRERRSRAMDDRERRI
                     TAWHESGHALLQVLCEHAEPLHKVTIIPRGQALGATMSLPERDVLNRTRSELLDQLVV
                     LMGGRIAEKFFTADLSTGARMDIKMASEIARKMICSYGMSDRFGFQSFGDNQETLFLG
                     REVARNQAYSEETAKEIDAEVSRLVTESYARAERMLQDNRAKLERLVECLLEAETLDG
                     RDVESLIRQGRLPDEVSSLEDGQADEPASTDPAVPVDKETPQDKPDEPPEFLL"
     gene            complement(15489..17081)
                     /locus_tag="PLZ74_02980"
     CDS             complement(15489..17081)
                     /locus_tag="PLZ74_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOR97316.1"
                     /translation="MLFGWFKKGAPKREIVVNVEKLETRVAVVENGRVEEFQVEHPTE
                     ERLVGSIYKGRIQNLEHDLQAAFVDIGMKKNAFLHYWDMLPDDDSRLDDDEENVRPSR
                     RRRISNDEIAKRFPPGSELVVQVTKGPIGTKGPRVTANLSLPGRYLVMMPGTRLRGVS
                     RKIGDAKERQRLKKILDRLPLPDDAGLIVRTVGAGASKRAFVRDLRNLWESWNELQDN
                     IKNLRAPACVYQEPDLVERVVRDWLTEEINRVTIDDGEAYARIREVAGRISRRAKHVI
                     AQYEGQLSIFEHHGIERQLEEAFRRKVNLQSGGYLIFDETEALIAIDVNTGRHRGKGS
                     QEDAILEVNLEAVEEVARQLRLRNIGGLVVVDLIDMKSRKHQNTVYKAMKQALKRDRA
                     RTNVLPISELGIMEMTRQRQEESLLSQMYSDCPYCRGHGLIKSPLALSVDIQRQIAVL
                     MRKARHEHEGQGLVELQIVVAPSVLDRLRTEDEAFLVELQRRYTGRLTFKSELHRHPE
                     SFCIADGQSGKILYAVGEQGAR"
     gene            complement(17129..18301)
                     /locus_tag="PLZ74_02985"
     CDS             complement(17129..18301)
                     /locus_tag="PLZ74_02985"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOR97317.1"
                     /translation="MLKQILLRFQRLNPLLLTCMIALIVIGVAFVYSACSIREDPELR
                     MLYLRHAKIGCVGLVVYLALAACPFATLLHWRWVAYGACLVMLILVPFIGETTMGGRR
                     WLFGIQPSEPAKVAIILVLAQLYGHPDAKRGVGAFLVTLLVVGIPAVLILLQPDLGTA
                     MVLVPTVLAMLFAANVAPRWVWSCVLIGGVAVGLLLGMIYAAEKADLTPEKREALIAA
                     THLKPHQVRRLQVFLFPDKDVLSSGYNRRQSEMAVGSGGMRGKGYLKGEQYMLGYLPP
                     SVSSNDFIFAVLAEEAGFVGSLTVLLLFLGLFWSGLWVAGRSQDNAGRLFCVGVISLL
                     FSHMFINIAMTIGMMPITGLPLPFISYGRSFMLSMMAALGGVQSVSIHGRESGIRF"
     gene            complement(18303..>19039)
                     /locus_tag="PLZ74_02990"
     CDS             complement(18303..>19039)
                     /locus_tag="PLZ74_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOR97318.1"
                     /translation="ANLSLVPVGADGAIALRKAIEQSCNSYFCHIGYTVGYEAIRTEA
                     AKLGLGQPTGIDLPSENRGLLPTDEWKRKQLKDRWRPGDTCHIAIGQGLLITTPLQMA
                     LLTATFANYGKLCRPHVARHAPESVRDMGWPADALKWVRTGMRDVAELGTGRRVRVRG
                     LEIAAKTGTAEFDAGGVRRKNTWVTAFAPFEQPTVAMAVMVEDGESGGLTVAPIVREV
                     LVSIFGEAPPEDAAAEPAAMPDVRGD"
BASE COUNT         3714 a         6436 c         5414 g         3375 t
ORIGIN      
        1 atgtcatccg cgagtacggc gcggacagca tgcgcctgta tgagatgttc atgggtccgc
       61 tggaggcggt caagccctgg aacaccaagg gcgtggaggg cgtgttccgt ttcctgaaac
      121 gcgcctaccg gatggtcacc gaacagccga tcaccgaaga tcccctgcag ccggagcaac
      181 tccggatgct gcacgccacc gtgaaaaagg ttaccgatga tctggaggac atggggttca
      241 acaccgccat cagccagctc atgattttcc tgaacgcgtt ttccggtgcc gacaagccct
      301 tgccgcgttt ggccgccgaa accttcgtgc tgctgctgtc cccctttgcc ccgcaccttg
      361 gcgaggaact ctggcagcat ctcgggcacg aaaaatccct ggcctatgca ccctggcccg
      421 tctatgacga atccctgctt cagctcgatg aggtggaaat cctcgtgcag atcaccggaa
      481 aaccgaaagc ccgtgtcatg atgccggccg atgcggatga ggaaaccatg cgtgagatgg
      541 ccctctccca atccgtgatc cgcgaggcgc tcaccggaaa aaccgtgcgc aaagtgatct
      601 gtgttcccgg acggctggtc aacatcgtgg caacttgaaa ccgcaccctg cgcgcgggtg
      661 acacagacgc ccgtcacacc gggacaaaaa gttacgcccg ttgcgttgct tttcgcgtaa
      721 gctcataggt gtcgcattgc cgcgagcggt tctcaagaca tgaacaactc ccgtggcatg
      781 ttttttgcta tgatcaatat ggcccccagg gaccagaaag ggtgtactga tgattcccta
      841 tttttatgat ccacggctga ttctcgtgat gttgcctgcg ctgattttgg cgggcatcgc
      901 ttccgtgctg gtcaaaacga ccttcaaccg ttacgcccgg gtacgggctt attcgggtat
      961 gaccggcgcg caggccgcag agcgcatgct gcgcgcgaac ggtgtgtttg acgtgacggt
     1021 cgaggaaacc ggcggattcc tttccgacca ttacgatccg acccggaagg tgctgcggct
     1081 ctcgccggac gtttaccgcg gcaactcgct ctctgcggtc ggagtcgcct gtcatgaggc
     1141 gggccacgcg ctacagcacg ccaacagcta cggcccgctg atgctccgca ccgctctggt
     1201 tcccgtgacg agtatcagca gttcactcgc cttttatgtg gtcgcggccg ggtttctttt
     1261 ccgtccgctt ctgcttgtcg ggtgcgcgat gttcgccgtg gcctttctct tctcgctggt
     1321 gaccctgccg gtcgagtgga atgccagtgc gcgggccaaa cagcatttgg tgacgtcggg
     1381 gatcgtctcg ccaggtcaag aggcggaggc cgggcgcgtg ctgaacgcgg cgttcctcac
     1441 ctatctggcc gcggcggtca gctcgctgat caccctactc tattatctgg cccgcgccgg
     1501 cgtgatcggc ggcggaagac gggattaaac aaacggagcg ttctgttttt atgagtaaga
     1561 aaacccacgt gttcaaggcg gagatccagc aggtgctgga tctcgtgatc cattccctgt
     1621 attcgaaaaa agagatcttc ctgcgcgaac tgatctcgaa cgcatcggat gcgattgacc
     1681 gtgcgcagta tctcggcctc acagacccga cgctgatcgc agatgccccg gcatggagga
     1741 tcgacattct ggccaaccgg gaggagcgga gtctgacaat cgccgacaac ggcatcggga
     1801 tgagcgaaga ggaactggac aagaacctcg gcgtgatcgc cagttcgggc accaaagcct
     1861 ttatcaaagc gttgcgggaa aaggagtcgg tggacatccc ggaactgatc ggtcagttcg
     1921 gcgtcggctt ctacgcctcg ttcatggtcg cggagcaggt cacggtcatc acgctcaagc
     1981 gcggcgccga tcaaaaggcg gtgaagtgga gttcgaccgg cgacggcgga tacacgctgg
     2041 aggatgcgga gcgggatcag cccggcacga cggtcgtgct gaaactccgg gaggatatga
     2101 aggaatatct ggacgagtgg cgcatccgcg agctggtgaa aacctactcc aatttcatcg
     2161 cctaccccat ccggttcagc gacaaaggcg agatgcccgc cgaggacagc gagccgctca
     2221 acaccatcaa ggccctttgg cgcaggccga aatcggagat caaggaagag gagtacaacg
     2281 agttttacac gcacctcagc catgatcacc ggccgccgct aaagaccatc cacttcgctg
     2341 tcgagggcgt gaccgaattc cgcgcgctgc tctttctgcc gaaagaagcg ggcatggatt
     2401 tgctcctgcc ccacaagcag cgcggtctta gtctctacgt gcggaacgtg ttcatcggtt
     2461 ccgatttcga cctgctgctc cctgattacc tccggttcgt caagggcgtg gtggattcga
     2521 gcgacctgcc gctgaacgtc tcccgcgaga tgctgcagga cgacgcgctg atccggaaga
     2581 tcaagagcaa cgtcaccggc aaaatcctcg caaccctgac cgagatgatg cgggatgaac
     2641 cggaagcgta ctccactttc tacacctcgt tcggccggat tctcaaagag ggactgcatt
     2701 gggattggga gaacagcgaa aaactgaagt cgttgctcat gttccgttcc gcgaaacagg
     2761 agaacaacgg gttcctgtct ctgaaagcgt atgtcgagtc catgcccgaa agccagaagg
     2821 atatctacta cctgctctcg gatgatctcg cgaccgcacg ccaatccccg cacatcgaag
     2881 cattgttgca gcacggttac gatgtgttgt tctttaccga tgccattgac cagtgggttg
     2941 tggagcgcct gagggaattt gacggcaaaa aactggttgc cgtggacaaa ggcactctgg
     3001 aactcggcac cgaagcggag aaggctgaaa cgaaaaaacg gatcgagacc gctgacgccg
     3061 aattccggga actgagcgcg ttcctcaaag aacacctcaa ggaaaaggtc ggcgaggtga
     3121 aactctcgac ccgtctgacc gattccgcct gctgtctcgt ggcggacgaa catgcactga
     3181 acgccagcat ggaacgcctg atgcgcgcca tgaaccaaga ggtgcccaaa caactccgca
     3241 cactggagat caacccggat catccggtgc tcaagcggat gaggcagatg ctggaaacgg
     3301 acaagtccga tccgaaactg gccgattacg cggatctgct tttgggtcaa gcgctgctca
     3361 gcgagggatc ggccctgccg gacactcaac gtttcacgcg gctggtctcc gaactcatgt
     3421 cggcgtcctg accgcaaacg cttccagcga cgagacaatc ggatagagtt gttcgtggta
     3481 ccagagcacc gagaagtaga ggccgatcgg ggttgcctgc ggtacgtttc ctgaccgcca
     3541 cgcctccttg agtctgttgc gtccgcgttc ggctgccgca cggcagggag catccccgcc
     3601 aagcagcgcg atcacagcca gggccgtctc ctcgacggtc gccgtctttc ccgcgctcca
     3661 tccaccgtcc ggtttctgac tccggatcag ccaacgctcg cccgctgtgc gcatcgcctc
     3721 cgcctctccg gcccatccca cggcatcagc ggcatagtgt gcggcacgca gcgcatccgt
     3781 cacccgcgcg gttcccacca ctggattttg tccgtgctcc gcatcctgat ggccgaacca
     3841 aagcggaatc cagctcccat caggctgctg ctcttgtttg agataaccca gaacgcgccg
     3901 gatggcggaa tccaccttac gctccgcgta cgacggaaga gccgggtcgt tgcgccaggc
     3961 cgcccatgcc gccaaggcgt gcgcactgat atcgggacag ctccggtcga acggcaaccg
     4021 gccccatccc cggcagaacg tgggaagccc cccatcccga ttctgcaagt ccagtaacca
     4081 ccgtacgcca tcgcggaccg cagcgcgaaa atcgaccgcg catccggcgt cccgcaggcg
     4141 tttgagtgcc accagcgcac ctgccgtatc gtccgcgtcc ggcacaccgc ccggcagatc
     4201 cgtccatgcc cagccgccgg gcgccgcacc agtgaagggg tgacgttctg tcttctgcac
     4261 ctgaatcaac cacctcgcga tcggcgtcag cgcctcggcc tccacccttc ccgcacccgc
     4321 cccctcccga tacgcggtta tcgcgcgcgc cgccagcgat gtcacccaag tgcgcagatt
     4381 ggagtcgatc gcccagcctc cgcccgcgcg cgccgaatgc cgcaggaacg caagtccctt
     4441 gcccaccacg gcgtgcccgc cgaaaccggc gtgatgcaac gccaagacca cgaaagcggt
     4501 cagcggaatc gcatccagat atccgccgtg gtccggttgc agcgcggcca gttttcgcag
     4561 cagcgcggga gcgagaacac gcccccatgc cgcaatccgc cggttccgcg ccacacgcag
     4621 atggcggcac agccccacag cgatcaaggc gggcagcgcg taactcacca cctgcaaccg
     4681 gagaagccga tagagtcgct gcggcagaca ggccagcgca aaaggcaggg gcatgaccca
     4741 ttgccaagcc tgccgcgtgt cctgacacat ggcgagaaag gcctggatcg gcaccgcaaa
     4801 ggtccgatcc tccccgtagg tgcgacgcaa cgcgcgcgcg accgaagcgg gtgaccgcgc
     4861 ttcgccaagc tgccggtcga tccactctcc ggcgcgagcc accgttttcg catgacgctc
     4921 acccgcaaac cgttgcagtg ccgcatgaac caacagggtc gtgctcagat tcgaccggct
     4981 gtgcggggta tccccccacc cgccgtccgg attcacgtga cacgccagcc aattcaatcc
     5041 ggaatccgcc tgcacccggt cgtcggcctc ccccgcatcc gccaaggctg cggcagccag
     5101 cgccgtggcc aaagcgctct ccgccagctc cccgctccag cttccgccgc cggggtcgcg
     5161 caagcgccag agatgacaaa agacctgctg accttcctcc ccgtccgcag catcgcatcg
     5221 gttcttcatg attggaagtg tagcatgtcg tgactgcggc gacgagcttt ttcaggcctt
     5281 gccgaatgtc gacgtacgcg aaaacgagga cggggacgag gacgaacccg aacccgatag
     5341 cgataaaccc ggaatcagga gcccgaacgg acttgccccg ttgaaacgtt tcaactatga
     5401 tgacttcaaa acccggatgg tatgaatgtt gcttacggcc cgcaggccca agagtccggg
     5461 agaggatcat tatggctgtc ccgtccctga gtttaagtca gcagcagcgt caggtcatga
     5521 cgctggcgcc gcaacttcgg caatcgttgg aaatgctcca gatgccggtg atggagttgc
     5581 gcgcggcgat cctcaacgaa atgtccgtca accccacgat cgaggaggtg gaggatccca
     5641 gcgaagtgtc gctggccacc gtgaccgcgc cggaaccgca gcagcagagt gcggctgatg
     5701 aggaactgga tttcaatccc gatatcgacg cgatcctgcg gcaagatgac gagtggcgcg
     5761 actacttcat gcagggcatg gaaaatgccc cctcgtacga ggaagagaat gaaaagcggc
     5821 aatacctgct ggactcgatc cgccagaagg cttcgctgca ggattatctg ttggagcagc
     5881 ttacgctcac ggatctccag ggcgaggact atgatctcgc agtgctgctg atcggcaaca
     5941 tttcggatga cgggtatttc accggaagcc tgccggacat catcatggtt tcgggccgca
     6001 gcgagcgcga agtgctcgat gtgctcaagg tcatccagac gtttgatccg ccgggcgtgg
     6061 gagcccgcac gctgcgcgaa tgtctgcttc tccaactcgc gctggttgaa gactccccct
     6121 gggaggatga ggcccggctg gtcatcgaca agcatctcga gcggctcggc gcgcatgacg
     6181 agaagtacct ctgcaaggtg cttgaactta cccgggagga gttggcgcat gtgaccgccc
     6241 tgatccgcag cctgaacccg cgtccgggac gggcgtatgt tcaggaaaag acggagtatg
     6301 tggagccaga ggtgtttctg gtccgcgaga aggggcgtct ggtcgcccgg gtcgacaacc
     6361 agaggctgcc gcacatccac atcagcaagc actatcggag tctgctggag gaccggaaca
     6421 ccccggcgga gaccaaaacc tacatccgtg agcgcatccg tgcgggcgcc tttcttatcc
     6481 ggagcctgta tcagcgccag gagactatcc ggaaaatcgc ccaggagatc gtggatgcgc
     6541 aggctgattt ctgggaaaag ggtgtgatgg cgctgcggcc gttgaccatg gccgaggtgg
     6601 cccgcaaggt cggggttcac gagaccacgg taagccggac ggtctccaac aaatacatgc
     6661 gcacaccacg cggcgtcttt gagatgaagt atttcttcac gcacgggttg aagtccgaag
     6721 acggggtgtc cgtctcgaac aaatccgtgc aggatcagat ccgccggctg gtggaggcgg
     6781 aagaccctac ggccccgctc tccgatcagg ctctggaaga gaagctttca gagcagggca
     6841 tcaaggtcgc acgccggacg atcgccaaat accgcggcat cctgaagatt ccgccctcgc
     6901 acaagcggcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgcggacgcc
     7021 tcggggatgc gtccctaccg gcaacctcaa cgttcggcgc tcccgccttc tgggggcttc
     7081 gggctccgcc ccggttagtc gatgtagagg tcaggcggcg gagagacggt ctcgaaaacc
     7141 ggcgcgggac cctcgctcac tcccgatccg gtcagccgga acagcaggct gatcccggcc
     7201 agaaggcaga tcaacaccac cacaccggca cccgccagca tcagcacgct tttatgcggc
     7261 agggtcacgg gcagacggcg ccccacatct gtccgcagct tcatgaaccc ctctttaaac
     7321 gcccggatgc gcgcattccg ccgggcgtgg gcgtcagagt cctcatccgc gaacccgcct
     7381 ggagcgcggt cattggctct tccgccgatt ttgaagatcg gcaacacgcg ccgttgggca
     7441 acggcgaatc ggtcggcact cttgcgtttt tccgcgtctg cggcatccgg ctgacgcggg
     7501 gtaacggacg gcgcgggatg gaagagatcc ggttcctgcg gcacatcccg ctctggcttc
     7561 tcctcctcct ctggccgcac cacccacccg aacagatcgt gcgcaccgtg gcgggctgac
     7621 cggacttccc gttccgcgcc cgtttcctcc gtcctggact tgaccgcaac cctgtccgca
     7681 tcttcggcag gcggtgctgc cgattccggc gcttccttca ccggccgcac catcacggcg
     7741 ggacggggct caatccgctg gcgctgcggc ccggcctccg gtgcctgacg cggcgtaacc
     7801 gccgacgcgg tttccgccgc atcctgctcg gcctcatggg gcacgtcgcg ggttcggatg
     7861 gcaggcacac gcgcaccagt gtacaagtcc atgaattcgc ggatcagcgg ctcgggatcc
     7921 aattccagca gttccgcata aagcttgata aagccgcgac cgtaaatcgg cgccgccacc
     7981 cggctgaact gctcacgttc cagatcctca accacctgca ccagcagatg cgtgctctcc
     8041 gccacatccg atggcgtcag cccgcgttgc aggcgcgcgt tcttcagaat ctctccgaat
     8101 gccatggtga cctcgctttc ctgttacacg cggggcgatt cgtccgcctc cgcctcctcg
     8161 tccggcaacg gagcgcccgc acgatccgtg ccaccgccca ccggaatttc cttgtccaaa
     8221 tcaataagaa tctcacgcga gttggagccc gcgtgcggac ccaccacacc gcgctcttcc
     8281 agaatgtcga tcaggcgggc ggcccgcgtg tacccgatgc gcaaccggcg ttgcagcatg
     8341 gtggtcgacg cgcgccgcgt catgcggatc acctcgatcg cgttcggcag cagcacgtca
     8401 tccgactcgc cgtcctccat cccctgctca cttccgccct cgccgtcctc ctcatccgac
     8461 aacgccgctc caggctcgct ctccctgatt ttttccagcc ggcccgagag cgcggcatcg
     8521 taacagggcg cggattgctc tttgacgtag gccacgacac gcgcgatctc ctcatccgag
     8581 acccacgcgc cctgcaggcg cgtcagttgc ccgccctccc gcaaaaacag catgtcgcct
     8641 ttgccgatca gctcttccgc acccgggctg tccagaatcg tgcggctgtc gttggactgc
     8701 gacaccttga aggcaatgcg tccgggaatg ttcgacttga tcgtcccggt gatcacatcc
     8761 acggacgggc gttgcgtggc gaggatcagg tgaatgccca ccgcacgcga aagcgaggtg
     8821 aggcgcgcga tggccggctc cacttctttg ccgaccgacg ccatgatgtc cgccacctca
     8881 tccacgacaa tcacgatgta cggcaaccgg tccggcgtct catcgactgc ggacgcctgc
     8941 ggagaaagcg cgtcctcctc cccgaacaga tcatcctgcc gagcgatcac ccggctgttg
     9001 tacgccacga tattacggca cccttccttc tgaagcaacc ggtaccgctt ctccatctcc
     9061 atgatggccc accgcagacc gaacgccact ttcttggggt cgttgatcac cggcaccagc
     9121 agatggggca actcgttgta acaggtgaac tccacgcgct tcggatccac cagaatcagc
     9181 cggagttgat ccggcgtgcg gctcatcaga aacccggtga gtatggcatt gaggcacacg
     9241 ctcttgccgg aaccggtagc cccggcgacc agcagatgag gcgctttcgt gaggtcgaac
     9301 accacatcgt cccccgcaac gtccttgccc aaaagcagcg gcacctcgca ggtgttgcgg
     9361 gtccaggcct cgccctcgat gatctcgcgg aaggtgatcg cgcgggcgcg acggttggga
     9421 atttcgatgc ccaccgcatt ctttcccgga atcggggcct gaacccgcag gctggtcgct
     9481 tccagcgcca tctgcaagtt ggcggtcagg tttgcgatgc gttcaacctt gatgttcggc
     9541 gcggggcgca gttcatattg cgtcaccaca gggcccggag tggtaccgac cacctccacc
     9601 ggcacgttga attgactcag ggtttcgacc agaatatcgg aggtttcggt gatgttgcca
     9661 tgatccgccg cccctttggg gatcgggttg agtaacctga ccgacggcag ctcgtaggcg
     9721 gccgggagcg ttgcggcagg ttccggttgc gcgacaacgg caggcgcggt ccgggtcccc
     9781 gccgcctttt ccggcacgac ctgttggggc tccacgcgcg gggcgggagc gggggcggcc
     9841 ggagcgggct cgcgctggcg ctcttgctca cgcgcctgac gcgcctcctc tttggcccgg
     9901 cgcgcggcct cccggattgc ctgacgctgc tgacgctccc gctcgcgacg cgtaagcccg
     9961 tcatccgcct ccgaatcttc cttttccgcc gtaccggggg gttccgtctt gtctgcggca
    10021 cgcggccgcg tcgtccacca accggtgacc catcccaacc cggccacgat attccgcaac
    10081 cccaccgcca gaaacaggga gcacagcata atggagagca tcaggatccc gccacccacc
    10141 ggactgaacc agcgagccaa caggcaggtc atcagcacat atccgaccgc cccgccggca
    10201 ttcggcttca ggttgaggcg ttccagaaca ggatcaaaca ctgtcgcgct accaagttgc
    10261 agcaaacagc aaagcgcgat caggaaaatc agcatccaga aggcccggcg gacaagacgc
    10321 acggcacgcc cgtagatcag catgacgctg aacagcagca ccatcaccgg aaccagccaa
    10381 agcgcgagac cgaccagact gtagccgatg aacaccgacc aagcgccgac gatgccgatc
    10441 agattcgagg ggggcgtcag gggcggcgcc tgcaaccagg agatgtcgcg ccaattgtat
    10501 gtcagaagac tcagcagcgg aaagagcgca atcggaaaga gcaacacacc ggtccaacgc
    10561 cgctgaatgc gtcgctccaa ctccatcctg tcctgttgtt ctatcaaccg ttccgacatg
    10621 gctctcactt ttgccccgcg ggcgtatcgg cgccgacaac cgacgctgtc acgggaatca
    10681 ggcggaccgt cggcggcaac gcccggacct cgctgacctc taatccctgt tgaaggtgta
    10741 tcatgaccgg caccccgttg gtctcccgcg acagcgggag attaccgctg accacggcgg
    10801 tcacctgctc cggcgtgacc gccttgacga tctcgccgcg tccgctcaac tcgacctcca
    10861 cacactccgg ctccacgtgc cagcgctgca ccatgtcatg cgggtatacc accaccggca
    10921 cgcgctccag tttcgcggtg gccttctcgc tgaggatctg cacctgcacc accatttcgg
    10981 gcggttccac ggtcgcgttc accacatcgc cgggcggcaa caggcgcaca cgcgtcgaaa
    11041 aggtttgcga gcggccgtcc acattgatgg gttcggtttg gatctgggtg acctcgggat
    11101 tcagcgtgct gagctggcgg cgcgaacccc gcacaaccgc attggtgagg tcgtacacca
    11161 actgcacgcg gccacgcgcc gtgccgatct cggcgggtcg cgcgatcata agcggaaggg
    11221 acatgggcac gtcaaacttg acgaccgcca cgttcggatc aaccttcacc acccggatcc
    11281 cgcgcacccc gcgcagattg ccgcgccgga ttttgaccgg caccgagtcc accagattcc
    11341 ccttcttctg ccgcggccgc accacgcaac gcgccacaga gagcgacaac tggttcacat
    11401 ccgtatagga gccgcgcagg gtcacctgca cggaacgcgg ctccacggat tcaatcgcgg
    11461 caccggcacc cgccgcatcg aactcggcct ccaccggaat cgagatgacc cgcacatgac
    11521 tgatttcggc ccggatcgag aaatagacca gaacggcgat caccaacgag agcaacttcc
    11581 aatacccgtt gttgagaaac acgatccgca ggatcgaaag caccttatcc ctttgcatga
    11641 tcgtcccccc cgaccgacat cgccaacggc ttcaaccact tttcgagttt cagggtcagc
    11701 cattcggtca cggtcgacgc gtcccctgtc tgctgcggca tggccacccg caaccagcgc
    11761 agaatcatct taccgatctt gtcatcctca tactgatgca gtctgccgtt gtaggccgcg
    11821 gaaatacgtc cggtctcttc ggacaccacg atcaccacgg cgtcggtctc ctcgctcagc
    11881 cccactgcgg cccgatggcg cattccggaa gccgcgagtt caccctgatt ggataccgga
    11941 aaaagacagt gcgcagaggc cacccggcca tcacgcagaa tgatgccgcc gtcgtgcaga
    12001 ggcagcggcg gggtgaaaag ggagaccatc agttcatgac tgaacaaggc gttcagctta
    12061 acccctgtct cctcataccc gcacagcgaa atgccgcgtt ccaccgcgat cagggcaccg
    12121 atccgcgccc gcgacagatt catcacgctc tggcagatca actcgggcac agaggactgc
    12181 tgccgcgtgt actggcgtga gaacaaccgt tgtccgccga ccagcgccag cacacggcgg
    12241 atctcctgct gaaacaccac caccatactc agcgccagat agatcaggaa aaaatacacc
    12301 acccacgcca gcacatcgaa gcggaagaga taggtgaaca gcagcaaaga gaccaccaga
    12361 acgcagaacc ccaacaacac ctgcgcactg cgcgtgcctt tcacgaaacg gatcagcgca
    12421 tagatcccca gatagaggat caggatctgc atcacgtcgt tgaagccgat ctggacacca
    12481 tcaaacatgc gtctcaccca ctgttgcctc cgccagacgg cggaccatcg ccaacgcctg
    12541 gcgggtagcc ttcacgtcat gcacccgcac caccgcggcc ccgtgcaacg cgcaccagac
    12601 cgccgcaccc acactgccgc ccacacgttc acgcgcgtca ggctcgccgc acacctcgcc
    12661 gacgaaacgc ttgcgcgagg cgcccaccag caacggtccc aatcgcgaca ggcgcgcggt
    12721 cgcggccaac aacgcgacag aatgcgccgt gtccttcgca aacccgatac cgggatccag
    12781 aagaatccgc tcgcgcccga tcccgctttc caccgcacac gccaacgcat cacgcagcat
    12841 tgcctccact tcgcccacca cgtcgcggta ttcggtcagg tcagacatgg tgcggggcgt
    12901 cccgcgcatg tgcatcagca ccacacccgc cccgctgtca cgcaccagac gaaccatctc
    12961 cgcgtcttcc gtgaacggct gcacggcatt gaccgcacag gctcccgcct ccagcgccgc
    13021 gcgggccacc gcagcatggc gcgtatccac cgtaaccggc acgccgctcc gctccgccag
    13081 catccggatc accggcgcca cccggcgcgt ctcctcttcc gtgctgacct cttccgcgcc
    13141 cggccgcgtc gattcgccac cgatgtcaag gatatccgcc ccttcctcca ccaaatccag
    13201 tgcgtgggcc acggcgcgtt cctcgtcgaa ccacttgccg ccgtcggaaa aggagtccgg
    13261 cgtcacattg acgatgccca tcacgagcgt atggcgcaac ggcaccacgc acccgcggca
    13321 cacccatgtg cgcccgctgt ctctctccac actctgcact cccatcctct cgcgtacgac
    13381 tacagtagaa actccggcgg ctcatccggc ttatcctgcg gcgtttcctt gtcgacagga
    13441 acagcgggat ccgttgatgc cggttcgtct gcctgcccgt cctccagcga cgacacctcg
    13501 tcgggcaggc ggccttgacg gatcaggctc tccacatcgc ggccgtccag cgtctcggcc
    13561 tccaacaggc actccaccaa gcgctccagt ttcgcgcggt tgtcttgcag catccgttcc
    13621 gctcgcgcgt acgactcggt aaccagccgc gagacctccg cgtcgatttc cttggcggtc
    13681 tcctcgctgt aggcttggtt gcgcgccact tcgcgcccca gaaagagcgt ctcctgattg
    13741 tcaccgaatg actggaaacc aaaccggtcg ctcataccgt acgaacagat cattttccgc
    13801 gcgatctcgc ttgccatttt gatgtccatg cgcgcgccgg tggagaggtc ggcggtgaag
    13861 aatttttccg cgatgcgtcc acccatcaga accaccaact ggtcgagcaa ttcgctgcgc
    13921 gtacggttca gcacatcccg ctcgggaagg gacatcgtgg cccccaaggc ctggccgcgc
    13981 ggaatgatcg tgactttgtg cagcggctcg gcatgttcgc acagcacttg cagcaacgcg
    14041 tggcccgact cgtgccaggc ggtgatgcgg cgctcgcggt catccatggc ccggctgcga
    14101 cgctcgcggc cccacagcac cttgtcgcgc gcctcttcca gatcggactg cccgaccgcc
    14161 cgcttgttgg cgcgcgccgc cagcagcgcc gcctcgttca gcaggttggc caaatccgca
    14221 ccggagaagc cgggcgtccc gcgggcgatt cggctcaggt cggcgtcatg cgccaacttg
    14281 atcttctcgg cgtgcatccg caggatctcc tcgcggccct tcagggtggg caggtcgatg
    14341 atgatctggc ggtcgaagcg gcccggccgc agtaacgccg gatccagcac atcggcgcgg
    14401 ttcgtggccg cgatgatgat cacgccttcc gaggtctcga aaccgtccat ctccaccagc
    14461 agcgcgttga gcgtctgctc gcgctcatca tgcccgccgc cgataccgga gaagcggctg
    14521 cggcccaccg catcgatctc atcgatgaaa atcaggcagg gcgcatgctt cttgccttgc
    14581 tcgaacatgt cgcgcacccg cgacgcgccc acccccacga acatctccac gaaatccgaa
    14641 ccgctgatac tgaagaaggg cacgcccgcc tccccggcga tcgccttggc caacaaggtt
    14701 ttgcccgttc ccggcggccc catgagcagc acccccttgg gaatccgtcc gccgaggact
    14761 tggaatttcc gggggtcttt cagaaactcg acgatctcct gcacctcttc cttggcctcc
    14821 cggatgcctg ccacattcgc gaacgtcagc ttcttcttgt cctggctcaa cattttcgcc
    14881 cggctcttgc cgaaactcat cgcgcccatg ttggccgatt tgatttggcg gatgaaaacg
    14941 aaatagagga tcaccaggat caggatcacc ggcaggatgt cgcggagcag cgcgccgaag
    15001 acattggtgt tgtaaaccaa cggacaggga atcttctttt cgagcagaaa ctgctcgaga
    15061 ttctcggacg ggatcaggtt gacacggaac cgctctttgg cggcaccctt gcggactccc
    15121 cggaggtagg tcatgccgtt cgcttcgcgc accaactcga ccttttcgat cagtccctct
    15181 tccaccagtc cccggaactc gagctggtcc atgggccgca tcgcgccttc gggtttgtaa
    15241 tagaacatcc ccagcatgaa cagcagcaac aggccgacga ccatccaggc gccgaaaacc
    15301 cgtttgggag gcagggcgcc gcttgtcgcg cccccctccg gcttgccccc ccggttcggg
    15361 agcgcgggag gttgattcgg cagctcgcgc ttgtgatcac tctctttcga catagggttc
    15421 atatcatacg ttcccaacgc gcgtggggca atgcgaaaac agtggaacgt tcaccgcgaa
    15481 cgcccccatc acctcgcgcc ctgctcgcca acagcgtata ggattttccc gctctgcccg
    15541 tccgcgatac agaaagattc gggatggcgg tgcaattccg acttgaaggt cagacggccg
    15601 gtgtagcggc gctgcaactc cacgagaaac gcctcgtctt ccgtgcgcag acgatccagc
    15661 acactcggcg ccaccacgat ctgcagctcc accagaccct gtccctcgtg ctcgtgacgg
    15721 gccttccgca tgagcaccgc gatctggcgc tggatatcca cactcagggc cagcggtgat
    15781 ttgatcaggc cgtggccgcg gcaatacgga cagtccgaat acatctggga cagcagactc
    15841 tcttcctgac gttgacgcgt catctccatg atccccaact ccgagatggg caacacgttc
    15901 gtgcgggcgc ggtcgcgctt gagtgcctgt ttcatggcct tgtagaccgt gttctggtgt
    15961 ttccgggatt tcatgtcgat caggtccacc accaccagac cgccgatgtt gcgcaaccgc
    16021 aactggcgcg ccacctcttc caccgcctca aggttcactt cgaggatggc gtcctcctgc
    16081 gagcccttgc cgcggtggcg tccggtattc acgtcgatgg cgatcagcgc ctcggtctca
    16141 tcgaagatca gatacccgcc ggactggagg ttgaccttgc gccggaacgc ctcctcgagt
    16201 tgacgctcga tgccgtggtg ctcgaaaatc gacaactgtc cctcatactg ggcgatcacg
    16261 tgtttggccc ggcgcgagat ccggccggcc acctcccgga tacgggcgta agcctcgccg
    16321 tcatcaatcg tcacgcggtt gatttcctcg gtcagccagt cgcgcaccac gcgctcgacc
    16381 aaatccggct cctgatacac gcaggccggc gcccgcaggt tcttgatgtt gtcttgcagt
    16441 tcattccatg actcccaaag gttccggagg tcgcgcacga aagcgcgctt gctggccccg
    16501 gccccgaccg tgcgcacgat caggccggcg tcatccggca acggcagccg atccagaatc
    16561 ttcttcaggc gctggcgctc tttcgcatcg ccgattttgc gtgacacccc gcgtaaccgc
    16621 gtacccggca tcatcaccag ataccgtccc ggcagactca ggttggcggt cacgcgcggc
    16681 cccttggtgc cgatcggccc cttggtcacc tgcaccacca gctccgaccc cgggggaaac
    16741 cgtttcgcga tctcatcatt cgagatgcgc cggcggcgcg agggacgcac gttctcctcg
    16801 tcgtcatcca gacggctgtc gtcatccggc agcatgtccc agtagtgcag gaacgcattt
    16861 ttcttcatgc cgatatcgac gaacgcggcc tgcagatcat gctccagatt ctggatgcgg
    16921 cccttgtaaa tgcttccgac cagacgttcc tcggtgggat gctccacctg aaactcctcc
    16981 acccggccgt tctccacaac cgccacgcgc gtttcgagct tctccacatt caccacgatc
    17041 tcacgcttcg gcgcgccttt tttaaaccaa ccgaataaca tacccaacct cctgactgga
    17101 cggggattcc gcgcacagcg cgccccgctc aaaaccttat ccctgattca cgtccatgta
    17161 tggacacact ctggactccc cccagcgccg ccatcatcga cagcataaag gaacgcccgt
    17221 aactgataaa cggcaacggc aacccggtaa tgggcatcat cccgatcgtc atcgcaatat
    17281 tgatgaacat gtgactgaaa agcagggaga tgaccccgac gcagaacaag cgccccgcat
    17341 tgtcttgcga acggcccgca acccacagcc cggaccagaa caggcccaga aacaggagca
    17401 gaaccgtcaa cgaacccacg aaaccggcct cttcggccaa taccgcgaaa atgaaatcat
    17461 tggacgaaac cgacggcggc agataaccca gcatatactg ctcgcctttc agataccctt
    17521 tgccgcgcat gccgccggac ccgaccgcca tctcggactg ccgccggtta tagccgctgc
    17581 tcagcacgtc cttatccgga aacaggaaga cttgcagacg ccggacctga tgcggcttta
    17641 gatgcgtcgc cgcgattagg gcctcgcgct tctccggcgt gaggtcggcc ttttccgccg
    17701 cgtaaatcat gccgagcaac agtccgactg cgaccccccc gatcaacacg caggaccaga
    17761 cccaacgcgg tgccacattt gccgcgaaca gcatcgcgag cacggtcggc accagaacca
    17821 tcgcggttcc gagatcgggc tgcagcagga tgagcaccgc cgggatgccc accacgagca
    17881 gcgtcaccag aaaagcgccc accccccgct tcgcgtccgg atgtccatag agttgggcga
    17941 gcaccagaat gatcgccacc ttggcgggct cggacggctg gataccgaac agccagcgcc
    18001 gaccgcccat cgtggtctcg ccgataaacg gcaccaggat cagcatgacc agacaggcgc
    18061 catacgccac ccaacgccaa tgcagaagcg tggcgaacgg gcaggcggcg agcgccagat
    18121 acacgaccag ccccacacag ccgatcttcg cgtgccgcag gtagagcatc cggagttccg
    18181 gatcctcccg gatcgaacaa gcgctgtaca cgaacgccac gccgatgacg atcagcgcga
    18241 tcatgcaggt gaggagcaga gggttcaggc gttggaaccg cagcaggatc tgtttaagca
    18301 tgtcagtccc ccctcacatc cggcattgcc gccggttccg ccgccgcatc ctccggcggc
    18361 gcctccccga agatggacac cagaacctca cgcacgatcg gtgccacggt caggccgccg
    18421 gattcaccgt cctccaccat gaccgccatc gccacagtgg gctgctcgaa gggcgcgaag
    18481 gccgtgaccc aggtgttctt gcgccgcacg cctcccgcgt caaactcggc cgtgccggtc
    18541 ttggcggcga tttcgagccc ccgcacccgg acacgccgtc ccgtaccgag ttccgccaca
    18601 tcccgcatcc ccgtgcgcac ccacttcagc gcgtcagcag gccatcccat gtcccgcacc
    18661 gattccggcg cgtgccgcgc gacatgcggt cggcaaagct tgccgtagtt cgcgaaagtc
    18721 gcggtgagga gcgccatctg caacggcgtg gtgatcagca aaccctgccc gatggcgata
    18781 tggcaggtgt cgccgggccg ccaccggtcc ttgagttgct tccgcttcca ctcgtcggtc
    18841 ggcagcagtc cgcggttctc ggagggcaga tcgattccgg tcggctgtcc cagccccagt
    18901 ttggcggctt cggtgcggat ggcctcatag ccgaccgtat atccgatgtg gcagaaatag
    18961 ctgttgcacg attgttcgat cgccttgcgc agcgcgatcg cgccgtcggc cccgaccggt
    19021 accaacgaga ggttcgcca
//
LOCUS       DAOSRB010000031        19003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3052_length_19003_cov_7.119221, whole genome shotgun sequence.
ACCESSION   DAOSRB010000031 DAOSRB010000000
VERSION     DAOSRB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19003
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3052_length_19003_cov_7.119221"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLZ74_02995"
     CDS             complement(<1..206)
                     /locus_tag="PLZ74_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR97319.1"
                     /translation="MTDAPARSAANRVVPRQYLFPFVLTTSLFALWGFVNDITNPMVA
                     AFKAVFIEISNTQSSLVQLAFYGG"
     gene            complement(377..1231)
                     /locus_tag="PLZ74_03000"
     CDS             complement(377..1231)
                     /locus_tag="PLZ74_03000"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOR97320.1"
                     /translation="MSLPGLTIIGESINDSIPSTHALFEARDLDAIARLAQTQAGLGA
                     AYIDVNVGPRAPDYMAEVVRAIQSATTAPLSLDSPDPLLARAALEAYDPAHGVPILNS
                     ISPLRMEMFELIALKPFRPILLASETVRDGMEIPCRTAEETVAAAALLLDAAHRHGIA
                     NHDCILDPGIAPLGTDTENNIHRLMGALRIIRDDPAFTGVHLSVGLSNFTVMLPPRRA
                     DGSPIRSALASAFLTRAMPLGLDMIIGSVKTPYTLLPEGHPALQCFDECLAADGFDVL
                     ERVQAFCE"
     gene            complement(1255..2166)
                     /locus_tag="PLZ74_03005"
     CDS             complement(1255..2166)
                     /locus_tag="PLZ74_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97321.1"
                     /translation="MNNLIPRVPQNPCRSRRPRGARRCLALLLLSAGLSAGAADRLPA
                     HLYPQNDAFRKEMAELRARGITLTDYHIHIRGGMTPQKAAERQEQSGIRSAILENHGR
                     EWPICDNARLAAFLDDCSRVQAHGKRLPVGIQVNDRDWHRTLDPALFARLDFVLADTM
                     IMGVTADGKPQRLWLPDVTIENPEAWMKEYVAHNLRILDEPISILANPTYLPPCISNQ
                     YDRLWTEARMRAVIAKAVSRGVALEIQAESPYPSPVFITLAKSMGARFSFGSNNKTDQ
                     TKNPARWFETLRKHDLRPDQLWTPQRP"
     gene            complement(2204..2635)
                     /locus_tag="PLZ74_03010"
     CDS             complement(2204..2635)
                     /locus_tag="PLZ74_03010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOR97322.1"
                     /translation="MTEPRRITLPYRVPYADTDQMRVVYYANYLTLFERARNELMRDC
                     GYTYRNLEAEGFALPVLEAHVTYHAPATYDDLLEITAWCERFQGVRLTIRCEVRRDGV
                     LLADGYTVHAHVNAATLRPTRPGKNLLTALGLVTAMPEEKG"
     gene            complement(2632..3438)
                     /locus_tag="PLZ74_03015"
     CDS             complement(2632..3438)
                     /locus_tag="PLZ74_03015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOR97323.1"
                     /translation="MTPNHLLAIASHATRRAGEYVLANLHRRGDVNTLLRTDVKHKLD
                     IEAQEIATETILAAFPDHAILGEETCDTPLPESDYVWVVDPIDGTINFFHGLPLWCCS
                     VAVRHRGQTVAGSVFLPELRQLYEATCDGPAYCNGVPLKVSSTDRLEHAMLATGADKW
                     DLGTYAFTFMRRVAEIAQRPRILGAAAVDLCLVAAGKLDGYFESGIYHWDMAAAGLIV
                     ERAGGTCEILREFPGYRIAFLATNGVLHAACRDALLPLLASSQPKTGAQP"
     gene            complement(3591..4787)
                     /locus_tag="PLZ74_03020"
     CDS             complement(3591..4787)
                     /locus_tag="PLZ74_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOR97324.1"
                     /translation="MIRLLPQLVLAVLCAGFPGAAETAPNTFGNHVVARGAFQNARIR
                     FEREQRGTVAFLGGSITEMNGYRPMVCSILTNRFPRTAFTFIQAGISSTCSTTGAFRF
                     GTDVLSQGRVDLLFIEFAVNDDQDAHHDRNACIRGMEGIVRQARRANPEMDIVITFFV
                     NEFILKTLQAGRTPLTIEAHEAVAAHYAIPTIHLAGEVAKRITAGSLTWKQFGGVHPA
                     PLGNALCASMIRELMDRAWSRPLTPESATVPAPMPPTPLDPLNYANGRFLDPAAARFG
                     QGWTLGIPDWQSLPGTKRPRFSAIPMLCATEPGSELTLAFEGTAIGAYLVAGPDAGTV
                     AVRIDGKPFPDVNLDHAYSKGLHYPRTVMFGTGLDAGPHTLSLRIAPTTSSTGHAIRI
                     MRFAAN"
     gene            complement(4798..5310)
                     /locus_tag="PLZ74_03025"
     CDS             complement(4798..5310)
                     /locus_tag="PLZ74_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOR97325.1"
                     /translation="MTKRKKTIADPRPDWDTYFMEIARVTAMRANCSRRKVAAVVVSD
                     NRIISTGYNGTPRGVTNCFAGGCARCAGTAPSGSSLEECICVHAEQNAICQAAYYGTR
                     LAGATIYVTISPCLTCAKLIINAGIKEVVYDGDYAFTEQTERLLQEAGVTCRRYVSGA
                     GVCLPASQGE"
     gene            complement(5310..6632)
                     /locus_tag="PLZ74_03030"
     CDS             complement(5310..6632)
                     /locus_tag="PLZ74_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97326.1"
                     /translation="MKLTRRTMLKRTALAGMPFLLPSSIRAATTSPNSRPTLGVIGMG
                     IRSRQLLDSFCHDMQVLAICDADTTRREDGCGRVNAYYARFPEKGTGGCVALRDFRDL
                     LARRDIDAVYIGTPDHWHAFQAIAALRAGKDVYCEKPLTHNIHESLALMRVVRETGRV
                     LQTGAQQRSDKTFRVACELVRNGCIGKIERIDCSFGGPARPYNLTAEPLEPGLDWDLW
                     CGPGPLVPYNSVLSPRGVHSHFPRWRGFREFGGGSTADLGAHHLDIIQWALDRDASGP
                     VEARPPAEAKAGFGAELVYADGVTVRHISGGFSIRFYGTEGVIDVGRGTFRLERQGQE
                     IARFLKREDGSSCEAQVLKAERAFLANAKVKLYNSRSHLSDFLACMANRKAPISNVEV
                     GARSAICCHLLNQAYYNREPIRWDPEGLRLTGGNPAWLTREYREPWSV"
     gene            6762..7523
                     /locus_tag="PLZ74_03035"
     CDS             6762..7523
                     /locus_tag="PLZ74_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOR97327.1"
                     /translation="MMGEVLKNIRVVLVGTLYHGNVGAVCRAMANMGIGDLVLAAPQL
                     CDDPAEAERMAVHATDVLKRRRVTASLAEAVADCAFTVGTTARGGLYRQHVKTPREWA
                     PELLRLAEQGRVALVFGREDKGLLNDEIGLCTHLIRIPVALAYTSINLAQAVMICCYE
                     LFAAVGIYEPPVEKSAPAVAARKTKMLELWRAMLLQIGFMKEDKADHMMQGVQRIFSR
                     GVYSDDDVAIMMGVARQADWASRNAPVSRSGKTET"
     gene            complement(7563..8885)
                     /gene="rmuC"
                     /locus_tag="PLZ74_03040"
     CDS             complement(7563..8885)
                     /gene="rmuC"
                     /locus_tag="PLZ74_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOR97328.1"
                     /translation="MNLFILTFLLLALVLVLLVVLIVRTGRDAPDRAALARLQTRDAD
                     YQEATRLLREQSERLESLQVENARLTAELEHERRNAAEKLRLLQDAETRLKTEFENLA
                     NRIFEDKGKAFTEQNRERLTGLLQPLKEQLDGFRKRVDEVHKDGTEQSARLLEQVRQL
                     QELSNRVSDEANQLARAIKGDAKRQGDWGELIVERLFEASGLEDGREYEAQPLLRTED
                     GAARRPDFVVHLPQGKSVIVDSKVSLTAYERYCNAETEEDRRGALEAHVQSIRRHLEE
                     LRGKNYTALLGNRTLDFVLMCIPIESAYQTALQADPKLLYDLAQTRVVITGPSTLMIT
                     LKLIAQIWRREKENRHAEQIADRAGRLYDQVTLIVEAMTAAKKNLAETTNCFDLALAR
                     LQTGRGNLVGRIEVLRKLGAKVSKALPPQVIEAALATEPAENDEPAQP"
     gene            complement(8975..10825)
                     /gene="recD"
                     /locus_tag="PLZ74_03045"
     CDS             complement(8975..10825)
                     /gene="recD"
                     /locus_tag="PLZ74_03045"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOR97329.1"
                     /translation="MKQNMPSTTPSRDLGNLCAALQACDRLEPYARYFADAVCRLAPV
                     GLGGAPAAWLAAALTAQNTVVNKHVCLALDPGLRLTDLLPPESIATDAPDAEDLFSAQ
                     LDDLAETLRGKECAFAVTVDPPSDVVPHTPLVLEQNRLYLQRYWLAENTLASQLNARA
                     TETPQSAPDRVEPQTLTRLTLDDAQRAAIRQALTHRLCVITGGPGTGKTTLVSVVLAA
                     LLRETPRRIIRLCAPTGKAQARLKEALDGEINRHLRLEHDEDLRQRLLALETSTLHRL
                     LRARPPLGRFAFHASNPLAADTLIVDEVSMIDLPLMVSLLTAIPSACQVILLGDSDQL
                     AAVEIGAAMGEMCAAWEGTRAVARLTRSHRFDPERGIGRLKDAVCAGDADRAWEILKT
                     GDDALGHAASPAGYANLETALASHLRDHPVRAYLAAPTPAESFARFDAFRILCATRHG
                     PCGVDAANRYVQTLLGVAPYAHGYPVMVTVNDASRNLFNGDIGLCLRDPQHNGTRVWF
                     PDPERAAAEPHSPPGRALRSFATADLPEHEPVFAMTVHKAQGSGFDDVLLLLPPYPTP
                     VLTRELLYTGITRARTRCTVWANEPIFKDAVRNRTRRMSGLQNKLARLDG"
     gene            11140..12360
                     /locus_tag="PLZ74_03050"
     CDS             11140..12360
                     /locus_tag="PLZ74_03050"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97330.1"
                     /translation="MGIIGCGNQSEHDLKQWFAMDDVQVVSVCDVNRGSHGYRSPQQF
                     LGREPVRDLVNQAYAQRAASGTFKGCGMVADFRELLARRDIDTVAIITPDHWHAIQTI
                     QAAAAGKDIYCQKPLGLTVQDGQEMIRAVRKHNRVLQTGSQWRSTPSLRRFYDLVRGG
                     AVGTPRKAVTYVARNNFTGPGPGWQPMPVPQGFDYDMWLGPAPDAPYHKDRCFYRFRF
                     ISAYSGGQTTNFGHHAIGVAMWALGLDEIGPETVWDMGAEWPPAGDLFDTATKVHFGC
                     RYPGGFELECVSSPKSFGVRVEGDKGWIETNGFKIQSSSPDLVWNPTNVRAEAEFFNH
                     YRNFIDCVKSRGTPVESVVIGHRISTICHIGNIAMKLSRKIRWDAQAEQFVGDDEANA
                     MLRSRHRAPWSFSV"
     gene            12552..14534
                     /locus_tag="PLZ74_03055"
     CDS             12552..14534
                     /locus_tag="PLZ74_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOR97331.1"
                     /translation="MKKIMLAAATVVSIVCGAETCAADHPITENERPGREATEWSMSN
                     NYNCRDIGKLPRVMLIGDSITAQYQGAVGRELKGKAYYSYWASSYCVASAMHRTLLKA
                     YLTDLKYDVIHFNNGLHSLSTDPKVWARALKAALLLCRELQPQAKLIWCNSTTVEGEG
                     MNEKVIRLNHAALEVCKELGIDLIDDQFSLVAAQPDAHSDAYHLKGPVKELQAKQVAE
                     ICLRALKPAVSVVDSGAKGDGVHDDTPAFQRAINAAAADKGTVEVPRGVYFLKALEMK
                     SGVTLHLAAGAHLKGSRRIEDYGEGKDLYGKRLGMIRFLGIRDAAVIGELGSLIDGSN
                     SSSPTEFEARGVHLFHVTGSTNLLFRGYTVVDAGNYAHWFRHCADVRFDHVRIEGGHD
                     GVHFWNSSRVTVEDCEFNTGDDCVAGLDYRDLVVRRCKLNTPCQCLRLGGRRILVEDC
                     ELTGPGKHPHRCTLPRQAVLDGLEPAGEGRRYNLAAFLYYCEKDGDVKEDSGEIVVRN
                     CRVRNVQRFFHYNFDGSEIWAQGRPLRDIRFENVTAENVDFPLVAISTLECPTRLTLE
                     NCSIAFTKPVPEFIRTAAFGKLTLKNVRLSGVEGPLVRAWGRPPDVVAENVTGASGIK
                     EGAASEKIDAHPILGFLMDYPEHEMSPLNPKNLKRK"
     gene            complement(14578..14943)
                     /locus_tag="PLZ74_03060"
     CDS             complement(14578..14943)
                     /locus_tag="PLZ74_03060"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOR97332.1"
                     /translation="MDWYALADEEVRTVYRDLPDDLRTCLARLTVTLDSRPAPSERSE
                     PGDRDGLLGLFTGPGFAESEDSDMPMPAAIRLFVENLRDEARNDPLLFRQEVRTTLLH
                     EFGHYLGWDESDLAARGLA"
     gene            complement(14951..15571)
                     /locus_tag="PLZ74_03065"
     CDS             complement(14951..15571)
                     /locus_tag="PLZ74_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97333.1"
                     /translation="MTRIIPLSLCLLLAASGCAVFGKRANDTGSIPCHYRFKMLSRSE
                     DQMRVEAAIQSVAVGTVTRAGTAAYPEYRFRVARLADLDTLHPKLMFVESASIWSRDR
                     KQALNLKAAEVGAVFDSTDVSASAATTVTLSVKPGSRLYYKMPGGTETEITSRVDRNG
                     QVRFPVTLREGQKFVYVRAVKDNVTRYIRVNLFTNELRDLTKREYR"
     gene            complement(15568..16347)
                     /locus_tag="PLZ74_03070"
     CDS             complement(15568..16347)
                     /locus_tag="PLZ74_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR97334.1"
                     /translation="MNRTVLITGAARRIGEALARELARAGWNVVLHAHHSIRAADALR
                     DELRAQGVQAWRVRGDLQPPGGADAVFDAALAEAGGIDAVVNNAACFERQSLADATPE
                     AFDALWRLNTLAPIRLTQRLAAHTSARQTAGCVVNLLDQRIALPHAGCLPYLLSKQAL
                     ATFTTCAALEWAPSGLRINAVAPGAVLTPVTEADREPAGGFPLGRRPTVRHIAEAVRF
                     LLEAESVTGQILYTDGGQHLMGQAHLATPPDFHKPESGVHA"
     gene            16435..17331
                     /locus_tag="PLZ74_03075"
     CDS             16435..17331
                     /locus_tag="PLZ74_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97335.1"
                     /translation="MNLRVAERDFKYYIFDWDNNILHMPTRIHLEKRMPDGSWQPHSV
                     STAAFAVLRKDPERYRPPGGVWPRAFVEFQDAHGEGESRFLRDTRDALRRVLSGEIKP
                     GPSFETLRETLREGRLFAIVTARGHEPDSIRKAVQLFIDMVLTHEERQEMMANLRGYR
                     ACFDGLTTFGTDEEELEYYLSLNRYHAVTHPGFLQWMSAEEGDAGEADTASAKQFAIR
                     DFVEHVFRLLSRTGAESLRRPISVGFSDDDPENVAAVERYIQEELSRRFPAIKFCVYD
                     TSDPALKDGRKVTVAGQLDFGF"
     gene            17696..18892
                     /locus_tag="PLZ74_03080"
     CDS             17696..18892
                     /locus_tag="PLZ74_03080"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOR97336.1"
                     /translation="MKPQQTVLFLGDGMADEPIGELNGRTPLQVARIPNMDTIARNGR
                     SGTMLTLPAGFPTSSDVANMSVLGCDLATELCGRGPLEAASQGIALGPDDIAFRMNLV
                     TVDAEGILRDFSGGHIAQADAVESIRLLNEQLGGEAVRFYPGVSYRNLLILSGPQFSA
                     NISYDKPDDQHGQPVAKHLPRTKSEEGRFTAALLRDLIARAAEVLQGRTANGIWPWSG
                     GKAGAIHNFCERYGVRAAVISAVDVINGLGRCLGMDVLPVPGATGYIDTNYEGKADAA
                     LKALADGYDFVYVHVEAIDEVSHARDLQLKIKTIEDFDRRLVGRVMAGAGDRVAYCVL
                     PDHPVPVATGKHTRDPVPVAVWQPGVKPDAVAVFDEVAARRGALKHLKGADLMNLLLR
                     ATDPQS"
BASE COUNT         3406 a         5546 c         6443 g         3608 t
ORIGIN      
        1 tagccgccgt agaaggcaag ctgcaccagc gagctttgcg tgttgctgat ctcgatgaag
       61 acggctttga aggcagccac catcgggttg gtgatgtcat ttacgaatcc ccagagggcg
      121 aacagcgagg tggttagcac aaaggggaag aggtattgcc gtggaaccac acggtttgcc
      181 gccgaccggg ccggtgcatc tgtcatactg aactcctgat tgaatcggat tagaatgaaa
      241 acaccgcata ctgtactgga ttcgctacgc taaagaaatc caattttgtt caaagtccgc
      301 aattgcccgg acaggcggat tcccaacggt tccggatcgt gggtctggcg cgcgccgcgg
      361 atttccggta atggcctcat tcgcaaaacg cctgcacgcg ttccagcaca tcaaaaccgt
      421 cggccgcgag gcattcgtcg aagcactgca gggcgggatg gccttcggga agcagcgtgt
      481 agggcgtttt gaccgaaccg atgatcatgt ccagcccgag cggcatggcc cgcgtcagga
      541 acgcgctggc cagcgcggac cggatcggcg aaccgtccgc acggcgcggg ggcagcatga
      601 ccgtgaagtt actgagaccc accgagaggt gcacgcccgt gaacgcggga tcgtcccgga
      661 tgatccgcag tgcgcccatg aggcggtgaa tgttgttttc ggtgtcggtg ccgagcggcg
      721 cgatgccggg gtcgaggatg cagtcgtggt tggcgatccc gtgccggtgc gcggcgtcca
      781 gcaggagggc ggctgcggcg acagtctctt cggcggtacg gcagggaatc tccatgccgt
      841 ccctcacggt ttccgaggcg agcaggatcg ggcggaaggg cttgagcgcg atcaactcga
      901 acatctccat ccgcaggggg gagatggagt tcaggatcgg caccccgtgc gcggggtcat
      961 aggcctcgag cgcggcgcgc gcgaggagcg gatccgggga gtcgagcgat aacggcgcgg
     1021 ttgtggcgga ctggatggcg cgcaccactt cggccatgta gtccggcgcg cgcggaccga
     1081 cattgacatc aatgtaggcg gcgcccagac cggcttgcgt ttgcgcgagc cgcgcgatgg
     1141 catcgaggtc gcgggcttca aacagcgcgt gggtggaggg gatcgagtcg ttgatgcttt
     1201 cgccgatgat cgtaagtccg ggcagggaca tgggaactcc tttcgatacg ggggttacgg
     1261 gcgttggggc gtccagagct ggtcgggccg caggtcatgc ttgcgcaggg tctcgaacca
     1321 gcgtgcggga ttcttggttt ggtcagtctt gttgttcgag ccgaatgaga agcgggcacc
     1381 catggatttc gccagcgtga tgaaaacggg gctgggatag ggcgattcgg cttggatctc
     1441 caacgccacg ccgcggctga ccgctttggc gattaccgcg cgcatgcgcg cttccgtcca
     1501 gagtcggtca tattggttcg aaatgcaggg cggcaggtag gtggggttgg caaggatcga
     1561 gatcggttcg tcgagaatgc ggaggttgtg cgcgacatat tctttcatcc acgcctcggg
     1621 gttctcgatc gtcacatccg gcagccagag gcgctgcggt ttgccgtcgg cggtcacgcc
     1681 catgatcatg gtgtcggcga gcacgaaatc cagccgggcg aacagggccg gatccagggt
     1741 ccggtgccag tcgcggtcat tcacttgaat cccgacaggc agccgctttc catgggcctg
     1801 cacacgggaa cagtcatcca gaaacgcggc gaggcgggca ttgtcgcaga tcggccattc
     1861 ccggccgtgg ttctccagaa tcgcgctgcg gatgccgctt tgctcctggc gttccgccgc
     1921 cttctgcggc gtcatgccgc cacggatgtg aatatgatag tcggtcagcg tgatgccgcg
     1981 cgcgcggagt tccgccatct ctttgcgaaa cgcatcgttt tgcgggtaga ggtgtgcggg
     2041 aagccggtct gcggcacctg cggacaggcc ggcggacagc aacagaagcg caaggcaacg
     2101 gcgcgcgcca cgcgggcggc ggctgcggca aggattttgc ggaacacggg gtataaggtt
     2161 gttcatgtcg ggactataac acggggaccg cacgggatga aagtcatcct ttctcctctg
     2221 gcatggcggt cacgagtccg agtgcggtca gcagattctt gcccggccgg gtgggccgca
     2281 gggtggcggc gttgacatgg gcgtgcaccg tgtacccgtc cgccaaaagc acgccgtcgc
     2341 gccgcacttc gcaccggatg gtcaggcgca ccccttggaa ccgctcgcac caggcggtga
     2401 tctccagcag gtcgtcatag gtggcgggcg catgatatgt gacatgggct tcgagaaccg
     2461 gcagggcgaa cccctcggct tccaggttgc ggtaggtata gccgcagtcc cgcatcagct
     2521 cgttgcgcgc gcgctcgaac aaggtcagat aattcgcgta atagacgacc cgcatctggt
     2581 cggtgtcggc atacggtaca cggtacggga gggtgatgcg acggggttct gtcacggttg
     2641 cgctccggtt ttgggttgag aggaggcgag taacggcagg agcgcgtcgc ggcatgcggc
     2701 gtgcagcacg ccgttggttg cgagaaacgc gatgcggtat ccggggaact cgcgcaggat
     2761 ttcacaggtg cctccggcac gttcgacgat cagtccggcc gccgccatat cccagtgata
     2821 aatgccggat tcgaaatacc cgtccaattt accggcagcc accagacaga gatccactgc
     2881 ggcggcaccc agaatccgcg gccgctgcgc gatttcagcg acgcgccgca taaatgtgaa
     2941 ggcataggtc ccgagatccc atttgtccgc accggtggcg agcatggcgt gttccaaacg
     3001 gtcagtggac gaaaccttca gcggcacgcc gttgcagtat gcgggcccgt cgcaggtggc
     3061 ctcgtacagt tgccgcagtt ccgggaggaa gaccgagccc gcgacggttt gtccccggtg
     3121 gcgtaccgcg accgagcaac accacaaggg caggccgtgg aaaaagttga tggtgccgtc
     3181 gatggggtcg acgacccaga cataatcgct ctcgggcagc ggggtgtcgc acgtttcctc
     3241 tccgaggatg gcatggtcgg gaaaggcggc aagaatcgtt tctgtggcga tctcctgagc
     3301 ctcgatatcc agtttgtgtt tcacatcggt gcgcagaagc gtgttgacat caccgcgccg
     3361 gtggaggttg gcgagcacat attcaccggc gcggcgcgtg gcgtgggaag cgatggcgag
     3421 cagatggttg ggggtcatgg caaaaccttt tgacggggat ccgtcagggc ggatccggaa
     3481 ggccggttgc ttttccaatg ggtcgtgttc attgtgatca gtctaccaca aaaccggacc
     3541 ggcaaaccaa aaaaaccaaa cgtcgcaggt cgaatgtcgc gggtcgtgcg tcagttcgca
     3601 gcgaaacgca tgatgcgtat cgcgtggccg gtactggacg tggtcggcgc gatacgcagg
     3661 ctcagcgtat gcggtcccgc gtcgagaccg gttccgaaca tcaccgtgcg ggggtaatgc
     3721 aaccctttgc tgtaggcgtg atcaagattc acgtcaggga acggcttgcc gtcgatgcgg
     3781 accgcgacgg tgccggcgtc cggacccgcc acaaggtacg cgccgatcgc ggtaccctca
     3841 aacgccaggg tcaattccga gcccggttcg gtcgcgcaca gcatggggat ggcggaaaaa
     3901 cgcggccgtt tggtgccggg caggctctgc caatccggaa tgccgagagt ccagccttgg
     3961 ccgaagcgtg ctgccgcagg atcgaggaaa cggccgtttg cgtagttcag cggatccagc
     4021 ggcgttggcg gcattggggc gggtacggtt gcggattccg gagtgagcgg acgggaccag
     4081 gcgcggtcca taagttcgcg gatcatgctg gcgcagaggg cgttgccgag cggtgcggga
     4141 tgcacgccgc cgaactgttt ccaggtgagg cttcccgccg tgatccgctt cgccacctcg
     4201 ccggccagat ggatcgtggg gatcgcgtaa tgggccgcca ccgcttcatg cgcctcgatc
     4261 gtgagcggcg tccgtccggc ttgcagggtt ttgaggatga actcgttcac gaaaaaggtg
     4321 atcacgatgt ccatttcggg attggcccgc cgcgcttgcc gcacgatccc ctccatgccg
     4381 cggatgcagg cgtttcggtc atgatgggcg tcctgatcgt cattcaccgc gaattcgata
     4441 aagagcagat cgacccgtcc ctgcgacagc acatcggttc cgaaacggaa ggcgccggtg
     4501 gtcgaacagg tcgaggagat gcccgcctga ataaaggtga acgcggtccg ggggaaacgg
     4561 ttggtcagga tgctgcacac catcgggcgg tagccgttca tctcggtgat ggatccgccg
     4621 agaaacgcca ccgttccccg ctgttcgcgt tcgaaacgga tgcgggcgtt ttgaaacgcg
     4681 ccccgcgcca cgacgtggtt gccgaatgtg ttcggagcgg tctcggcagc accgggaaat
     4741 cccgcgcaca gaacggccag gacgagttgc ggaagcagtc ggatcatgag gtctccttta
     4801 ttcgccttgg ctcgcgggca agcagacgcc tgctccgctc acgtatcgac ggcatgtcac
     4861 gcccgcttcc tgtagcaaac gctcggtttg ttcggtgaaa gcgtaatcgc cgtcatagac
     4921 gacctctttg attccggcgt tgatgatcaa ttttgcgcag gtcaggcacg ggctgatggt
     4981 cacatagatc gtcgcgcctg ccagccgggt gccgtaatag gccgcctgac agatcgcgtt
     5041 ctgttcggcg tggacgcaga tgcactcttc cagactggag ccggagggtg ccgttcccgc
     5101 gcatcgcgcg catccccccg cgaaacagtt ggtgactccc cgcggggtgc cgttgtaacc
     5161 ggtcgagatg atccggttgt ccgacaccac cacggccgcc accttgcggc gtgaacagtt
     5221 ggcgcgcatg gctgtcaccc gcgcgatctc catgaaatag gtgtcccagt cgggtcgcgg
     5281 atccgcgatc gtcttttttc tttttgtcat cacacgctcc acggttcgcg gtactcccgc
     5341 gtcagccagg ccggattccc gcccgtcagc cggagcccct ccgggtccca gcggatcggt
     5401 tcgcggttgt agtacgcctg attgagcagg tgacagcaga tggccgaacg cgcgccgacc
     5461 tccacattcg agatcggggc cttgcggttc gccatgcagg cgagaaagtc cgagaggtgc
     5521 gagcggctgt tgtagagctt caccttggcg ttcgcgagaa acgcgcgttc ggctttcagc
     5581 acttgggcct cacaggatga gccgtcctca cgcttcagga agcgggcgat ctcctgtccc
     5641 tgccgttcga gccggaaggt gccgcgaccg acgtcgatga ctccttcggt cccgtagaac
     5701 cggatgctga acccgcccga aatgtggcgg accgtcacgc cgtcagcgta aaccagttcg
     5761 gcgccgaagc cggctttggc ctctgcgggc ggtcgggcct ccaccggtcc cgaggcatcc
     5821 cgatccaatg cccattggat gatgtcgagg tggtgcgccc cgagatcagc cgtgctgccg
     5881 ccgccgaatt cgcggaatcc acgccagcgc gggaagtgcg aatgcacgcc gcgcggcgag
     5941 aggacggagt tgtagggaac aagcggtccc gggccgcacc acagatccca atccagtccc
     6001 ggttccagcg gctcggcggt gagattgtag ggccgcgcgg gtccgccgaa cgagcagtcg
     6061 atacgttcga ttttcccgat gcacccgttg cgcaccagtt cgcacgccac ccggaaggtc
     6121 ttgtcagaac gctgctgggc accggtttgc agcacgcggc ccgtttcccg gacaacccgc
     6181 atgagggcca gcgactcatg gatgttgtgc gtcagcggct tctcacaata gacatccttg
     6241 cccgcccgca acgcggcgat cgcctgaaag gcgtgccagt ggtcgggtgt gccgatatat
     6301 accgcatcga tgtcccgccg cgcgagcagg tcgcggaagt cgcgtagcgc cacacagccg
     6361 cccgttcctt tttcggggaa acgggcataa tacgcattca cccgtccgca cccgtcctcg
     6421 cggcgcgtgg tgtccgcatc gcagatcgcg agaacctgca tatcgtggca gaacgagtcg
     6481 agcagttggc ggctccggat gcccatgccg atcaccccga gagtggggcg gctgttgggc
     6541 gaggtcgtcg ccgcccggat ggaagaggga agaaggaacg gcattccggc aagcgcggtc
     6601 cgcttcaaca ttgtccgacg agtcaatttc ataagtcccc tttcttttgg tgcatatacg
     6661 atacacgacc cgtttcccgt ttgtcgagtc gcgacacggc gcgtttttcg ttcgcgaccg
     6721 ggccgctgat acggtaaggt acctgaatcg acgggaggcg gatgatgggt gaggtcttga
     6781 aaaacattcg ggtcgttctg gtgggcacgt tgtaccacgg caacgtgggg gcggtctgcc
     6841 gcgcaatggc gaacatgggg atcggtgacc tcgttcttgc cgcgccgcag ttgtgcgacg
     6901 atccggcgga ggcggaacgc atggcggtcc acgcgacgga tgtcctgaag aggcggcgcg
     6961 tgacggcctc gctggccgag gcggttgcgg actgcgcgtt cacggtgggc accacggcac
     7021 gtggcggact ctaccgtcag cacgtgaaga ctcctcggga gtgggcgccc gaactcttgc
     7081 gcctggcgga gcagggccgg gtcgcgctcg tgttcgggcg ggaggacaag ggactgctga
     7141 atgacgagat cgggttgtgc acgcatttga tccggattcc ggtcgcactc gcctacacct
     7201 cgatcaatct ggcgcaggcg gtgatgatct gttgttacga gctgtttgcg gcggtcggca
     7261 tctacgagcc gccggtcgag aaatcggccc ctgcggtggc tgcccgcaaa accaagatgc
     7321 tggagttgtg gcgcgcgatg ctgctgcaga tcggcttcat gaaggaggat aaggctgatc
     7381 acatgatgca gggcgtgcag cgcatcttct cccgcggggt ttattccgac gatgacgtcg
     7441 cgattatgat gggcgtggcg cgtcaggctg actgggcctc ccggaacgct cccgtgtctc
     7501 gaagcggcaa gacggaaacc tgatccgccg agcggcggcg ggcccatccc tctgtgcgcg
     7561 cctcacggtt gcgcgggttc gtcgttttct gcgggttcgg ttgccagagc ggcttcgatg
     7621 acttgcggcg gcaaggcctt gctgaccttg gcgccgagtt tccgcagcac ttcgatgcgt
     7681 ccgaccagat tcccccggcc ggtttgaagc cgggccagcg cgaggtcgaa acagttcgtg
     7741 gtctcggcca gatttttctt cgcggcggtc atggcctcga caatcagggt gacctggtcg
     7801 tagaggcgtc ccgcgcggtc ggcgatctgc tcggcgtggc gattctcctt ttcgcgccgc
     7861 cagatctgcg cgatcagttt gagcgtgatc atgagcgtgg acggtccggt gatcacgaca
     7921 cgggtttggg ccagatcgta cagcagcttg gggtctgcct gcagggcggt ctggtacgcg
     7981 gactcgatcg ggatgcacat cagcacaaag tccagcgtcc ggtttccaag cagcgcggtg
     8041 tagttcttgc cgcgcaactc ctccaaatga cgccgtattg actgcacatg cgcctccagc
     8101 gcaccgcgcc gatcctcttc ggtctcggca ttgcagtacc gctcataggc ggtcagcgaa
     8161 actttggagt ccacgatgac cgattttccc tgcggcaggt ggaccacgaa atcgggacgc
     8221 cgtgccgcgc cgtcctcggt ccgcaacagg ggttgggcct catattcgcg tccgtcctcc
     8281 agtccggagg cctcgaacag ccgttcgacg atcaattcac cccaatcccc ctgccgtttg
     8341 gcgtcgccct tgatggcacg cgccagttga ttggcctcgt cgctgaccct gttgctgagt
     8401 tcctgaagct ggcgcacctg ctccagcagg cgggcggatt gctcggttcc gtccttgtga
     8461 acctcgtcca cacgtttgcg gaacccgtca agctgctcct tcagcggctg cagcagaccg
     8521 gtgaggcgtt cgcggttctg ttcggtgaaa gccttgccct tgtcctcgaa aatgcggttg
     8581 gcgagattct cgaactcggt tttgagccgc gtttcggcat cttgcagcaa ccggagtttc
     8641 tccgctgcgt tgcgacgctc gtgctccaac tcggcggtca gccgggcatt ctccacttgc
     8701 agcgactcca gccgctccga ttgttcccgc aggagccggg tcgcctcctg atagtccgcg
     8761 tcgcgcgtct gcaaccgggc cagcgccgcg cggtcaggcg cgtcgcgtcc tgtgcgcacg
     8821 atcagcacga cgagcaacac caaaacgagg gccaacagga ggaaggtaag gataaacagg
     8881 ttcatggtgt cagaaaaaga tgatgatcgg gtttcggggt tactcaacgc atgagcgatc
     8941 tccaaatgct gtgttgacga tacgctgtaa acggttatcc gtcaagtcgc gccagcttgt
     9001 tttggagacc ggacatgcgc cgggtgcggt tccgcaccgc gtctttgaaa atcggttcgt
     9061 tagcccagac ggtgcagcgt gtgcgggcgc gggtgatgcc cgtatagagc agttcgcggg
     9121 tcagtaccgg cgtcggatag gggggcagga gcagcagcac gtcgtcaaac cccgatccct
     9181 gtgctttgtg cacggtcatc gcgaacaccg gctcgtgctc gggcagatcg gccgtggcga
     9241 aggaacggag cgcgcgtccg ggcggggaat gcggttcggc tgcggcccgt tccgggtcgg
     9301 ggaaccagac gcgggtcccg ttatgctgcg gatcccgcag acagaggccg atgtcgccat
     9361 taaagaggtt acgggatgcg tcgttgactg tcaccatgac cggatatccg tgggcgtagg
     9421 gggcgacgcc gagaagcgtt tgcacgtagc ggttggcggc gtccactccg cacggaccgt
     9481 ggcgcgtggc gcacaggatg cggaaggcgt cgaaccgcgc gaaagactcg gcgggtgtgg
     9541 gggcggcgag ataggcgcgg acgggatggt cgcggaggtg ggaggccaaa gcggtttcga
     9601 ggttcgcata tccggcgggc gaggcggcgt gcccgagcgc gtcgtcaccc gtcttgagaa
     9661 tctcccaggc gcgatcggcg tcgcccgcgc acaccgcatc cttgagacgg ccgatgccgc
     9721 gttcagggtc gaaacggtgc gaccgggtca ggcgcgcgac cgcgcgtgtg ccttcccagg
     9781 ccgcgcacat ctcacccatc gccgcaccga tctccactgc ggcgagctgg tcgctgtcgc
     9841 ccagcagaat gacttggcac gcggacggaa tggcggtcag caggctgacc atcagcggca
     9901 ggtcgatcat cgagacctca tccacgatca gcgtgtcagc ggccagcggg ttggacgcat
     9961 ggaacgcgaa ccggccgagc ggggggcgcg cgcgcagcag gcggtgaagg gtggaggtct
    10021 ccagtgcgag cagccgttgc cggagatcct cgtcatgctc aagccgcaga tggcggttga
    10081 tctccccgtc caaggcctcc ttgagacgcg cctgggcttt gccggtcggc gcacagagcc
    10141 ggatgatccg gcggggggtt tcccgtagca gggctgcgag caccacactg acgagggtgg
    10201 tcttgccggt tccgggaccg ccggtgatca cgcaaaggcg atgggtcaga gcctgccgga
    10261 tggctgcgcg ctgggcatcg tccagagtga ggcgcgtgag ggtttgcggc tccacgcggt
    10321 cgggagcgga ctgcggggtt tcggtcgcgc gcgcgttgag ttgggatgcc agagtgtttt
    10381 cggccagcca gtagcgttgg agatagaggc ggttctgctc aagtacgagc ggggtatggg
    10441 gcacgacgtc ggacggcgga tcgaccgtga ccgcgaaggc acactccttg ccgcggagcg
    10501 tctcggcgag gtcgtccagt tgcgcggaga agaggtcctc ggcgtcgggc gcatccgttg
    10561 cgatggattc cgggggcaaa agatcggtca gccgcagacc gggatcgagg gccaggcaga
    10621 catgtttgtt caccacggtg ttctgtgcgg tcagcgcggc ggccagccag gcggccgggg
    10681 caccgccgag accgaccggc gcaagccgac agaccgcgtc ggcgaagtac cgtgcatacg
    10741 gttcaagccg gtcgcaggct tgcagcgctg cgcacaggtt tccgagatcg cgggatgggg
    10801 tggtggaggg catgttttgt ttcatggggc cggggttctg gcccgcgttc ggaaccatag
    10861 tcgtgtttct gccggtgccg gtcaagtgcg ttttgccggc ttgacaggtt tggggtaatt
    10921 ggaatatgtt gcttttgtag gttatggtat tcctattgaa acaggtcata aaacgggaaa
    10981 cgggaggagg gctgaagaat gtcgaagagg ctttccgatg cgccgggtgt caggtccaga
    11041 cgggggtttt tacgcacagc cgcttcaggg gccgtggggt ttccattgtt gatccgatcc
    11101 gagacctttg ggaagaacgc gccgtcgaac cggatcacga tggggatcat cggttgtggg
    11161 aaccagtccg agcatgatct caagcagtgg ttcgccatgg atgacgtgca ggtggtgtcg
    11221 gtctgcgatg tcaaccgcgg aagtcacggc taccggtcgc cgcagcagtt cctcgggcgg
    11281 gagccggtgc gggacctcgt caatcaggcc tacgcgcaac gggcggcatc cggcacgttc
    11341 aaggggtgcg gcatggtcgc ggatttccgc gagcttctcg cgcgtcggga catcgacacc
    11401 gtggcgatca tcacacccga ccactggcac gcgatccaga cgattcaggc cgcggccgcg
    11461 ggcaaggata tctattgcca gaaaccgctc gggttgacgg ttcaggatgg acaggagatg
    11521 atccgggccg tgcgcaagca caaccgcgtt ttacagaccg ggagccagtg gcgttccaca
    11581 ccctccctcc ggcggtttta cgatctggtg cgcggcggcg cggtcggcac gccccggaag
    11641 gcggtgacct acgttgcccg caacaatttc acagggccgg ggccggggtg gcaaccgatg
    11701 cctgttccgc agggatttga ttatgacatg tggctggggc ccgcgcctga tgccccatac
    11761 cacaaggacc gctgtttcta ccgtttccgt tttatcagcg cctactccgg cgggcagacc
    11821 acgaatttcg gccatcacgc gatcggtgtg gcgatgtggg cgctgggact ggacgagatc
    11881 gggccggaaa cggtgtggga tatgggtgcc gaatggccgc ccgcagggga tctgttcgac
    11941 accgccacca aagttcattt cggatgccgg tatcccggcg gattcgaact tgaatgcgtt
    12001 tcgtcgccga aatcgttcgg tgtgcgcgtc gagggcgaca agggctggat cgagaccaac
    12061 gggttcaaga tccagtcgtc atccccggat ctcgtctgga atccgacgaa cgtgcgggca
    12121 gaggccgagt ttttcaatca ctaccggaac ttcatcgatt gcgtgaagtc gcgcggcacg
    12181 ccggtggagt ccgtcgtgat cgggcaccgc atctcgacga tatgtcacat cggcaatatc
    12241 gcgatgaagc tcagccgcaa gatccgttgg gatgcgcagg ccgaacagtt cgtgggggac
    12301 gacgaggcga acgcgatgct gcggagccgc catcgcgcgc cgtggtcgtt ttcggtatga
    12361 ggcacggccc tcagacgggc gcccactcag gtttgctcca taatgtcaac cggttcggtc
    12421 cgcatggcgg tccatcataa ctgggaggtt cgccgcgcag ggccttgccg caaaggggcg
    12481 tggcccgctt tcgcgggcag gcggcgcctt gcggccgcgt tgatgtcggt ttttgataag
    12541 gaaacaggaa catgaaaaaa attatgcttg ccgcggcgac cgtggtgtcg atcgtttgcg
    12601 gagcagaaac ctgcgctgcc gatcatccga tcacggagaa cgagcggccc ggccgcgagg
    12661 cgaccgaatg gtcgatgagc aacaattaca actgccgcga tatcggcaag ctgccgcgag
    12721 tgatgctgat cggcgactcg atcactgcgc aatatcaggg ggcggtgggc cgggaactga
    12781 agggcaaggc gtattactcc tactgggcgt cgagctactg cgtcgcctcg gcgatgcacc
    12841 gcacgctcct caaggcgtat cttaccgatc tgaagtacga tgtcatccat ttcaataacg
    12901 ggcttcactc gctttcgacc gatccgaagg tctgggcgcg ggcgctcaag gcggcgctcc
    12961 tcctgtgtcg tgagctccag ccgcaggcga agctcatctg gtgcaattcg accacggtgg
    13021 agggcgaggg tatgaacgag aaggtgatcc ggctcaacca tgccgcgctt gaggtctgca
    13081 aagagttggg cattgacctg atcgacgacc agttctcgct tgtcgcggcc cagccggatg
    13141 cgcattccga tgcttatcat ctcaaggggc cggtcaagga acttcaggcg aagcaagtcg
    13201 cagaaatctg tctccgggcg ctgaaaccgg ccgtgtccgt ggttgattcc ggagcgaagg
    13261 gggacggtgt ccatgacgat acccccgctt tccagcgggc gatcaacgcc gcggccgctg
    13321 ataaggggac ggtcgaggtt ccgcgaggcg tgtatttcct caaggcgctg gagatgaagt
    13381 ccggtgtgac cctgcatctc gccgccggtg cgcatctcaa gggatcgcgc cggatcgagg
    13441 actacggcga agggaaagat ctgtacggga agaggctcgg catgatccgt tttctgggga
    13501 ttcgcgacgc tgcggtgatc ggcgagctgg gctcattgat cgacggttcc aattcgagtt
    13561 cgccgacgga attcgaggcg cggggtgtcc atctcttcca tgtcacgggg agtacgaacc
    13621 tcctcttccg cggttatact gtcgtcgatg ccggtaacta tgcccactgg ttccgccatt
    13681 gcgcggacgt ccggttcgac catgtccgca tcgagggcgg tcatgacggc gtccatttct
    13741 ggaattcaag ccgtgtgacg gtcgaggact gtgaattcaa caccggggat gactgcgtcg
    13801 cggggcttga ttaccgcgat ctcgttgtcc ggcgctgtaa attgaacacg ccctgccaat
    13861 gtctgcggct cggcggacgc cgcatcctcg tcgaggactg cgagctgacc gggccgggaa
    13921 agcatccgca ccgttgcacg ctgccgcgtc aggctgtgct ggatgggctt gaacccgcag
    13981 gcgagggtcg gcgttacaat ctcgccgcgt tcctctacta ctgcgagaag gatggggatg
    14041 taaaggagga ttccggagag atcgttgtgc gtaattgccg cgtgcgcaat gtccagcggt
    14101 tcttccatta caatttcgac ggcagcgaga tctgggcgca ggggcgtccc ttgcgcgaca
    14161 tccgcttcga aaacgtgact gccgagaacg tcgacttccc gctggtcgcc atctcgactc
    14221 tggagtgccc gacccgcttg acgctcgaga actgttcgat cgcctttacg aaaccggtgc
    14281 cggagttcat ccggacggcc gcgttcggga agctgacgct gaagaacgtg aggctctccg
    14341 gagtcgaggg cccgctggtg cgcgcctggg gaaggccgcc ggatgttgtc gctgagaacg
    14401 tgaccggcgc atcggggatc aaggaggggg cggcgtccga gaaaatcgac gcgcatccga
    14461 tcctcggttt cctgatggac tatcccgaac acgagatgag tccgctcaac ccgaagaatc
    14521 tgaaaaggaa atgagcggct gcggcaagga aggcgtccat cgcctctgtc ttctgtctca
    14581 ggcgagtccg cgtgccgcga gatcggattc gtcccacccg agataatggc cgaactcgtg
    14641 cagcagggtg gtgcggacct cctgccggaa gagcagggga tcgttccggg cctcgtcacg
    14701 cagattctcg acaaagagac ggatcgcggc tggcatcggc atgtcgctgt cctcgctctc
    14761 tgcaaagccc ggtccggtga agaggccgag caatccgtcg cggtcgcccg gctcactcct
    14821 ctccgacggt gcgggccggg aatccagcgt gacggtcagt cgggcgagac acgtgcgcag
    14881 atcgtccggc aagtcgcggt ataccgtgcg cacctcctca tccgcgagcg cgtaccagtc
    14941 catgtgcgcc tcagcggtat tcccgcttgg tgagatcgcg gagttcattg gtgaacaagt
    15001 tgacgcggat gtatcgggtc acgttatcct tgacggcgcg gacgtaaacg aatttctggc
    15061 cctcgcggag tgtgaccggg aaccggactt gtccgttacg gtccacccgc gacgtgattt
    15121 cggtctcggt cccgccgggc attttgtagt agaggcgcga acccggcttc accgacagcg
    15181 tgacggttgt ggccgcgctg gcggaaacgt cggttgaatc gaaaaccgcc ccgacctccg
    15241 cggccttcag gttcagggcc tgcttgcggt cgcgggacca tatcgaagcg gactcgacga
    15301 acatcagttt cgggtggagc gtgtcgagat ccgcaaggcg tgcgacacgg aaacggtact
    15361 cgggataggc tgcggttcct gcgcgggtca cggtgccgac cgccacggac tggatcgcgg
    15421 cctccacgcg catctgatcc tcggagcggg agagcatctt gaaccggtag tggcagggga
    15481 tgctccccgt gtcgttcgcg cgtttgccga acacggcgca gccggacgcg gcgagcagaa
    15541 ggcaaagaga gagcgggatg atacgtgtca tgcgtggact cctgattcgg gtttgtggaa
    15601 atcgggcggg gtagcgagat gggcttggcc catcaggtgc tggccaccgt ccgtatacag
    15661 gatctggccg gtcacggatt cggcctccag cagaaaccgc acggcttctg cgatgtgccg
    15721 cacggttggc cgccgtccga gcgggaaccc gcctgcgggt tcccggtccg cttccgtgac
    15781 gggcgtcagc actgcgccgg gcgccacggc attgatccgc agaccggatg gtgcccattc
    15841 gagcgccgcg caggttgtga aggtggcgag ggcctgtttg gagagcaggt aggggaggca
    15901 gcccgcatgg gggagggcga tccgctggtc cagcaggttg accacgcatc cggcggtttg
    15961 ccgtgcggat gtgtgggcgg ccagccgttg ggtcagccgg atcggggcca gggtgttgag
    16021 ccgccacagc gcgtcaaacg cctcgggggt ggcgtccgcg agggattggc gctcgaaaca
    16081 cgcggcgttg ttcaccacgg catcgatgcc gccggcctcg gcgagggccg cgtcaaacac
    16141 ggcatccgcc ccgccggggg gctgaaggtc gccccgcaca cgccacgctt ggacaccttg
    16201 cgcacggagt tcgtcgcgca gggcgtctgc cgcacggatc gagtggtggg cgtgaagcac
    16261 cacgttccag cccgcgcggg cgagttcacg cgcgagggct tctccgatcc ggcgtgcggc
    16321 gccggtgatg agcacggtgc ggttcatggt aatcgttcgg aacgaaaggt tgcttattga
    16381 caagcgataa gccgcatcat accttaaccc gcaatgcgac gtcatgcaca aaacgtgaat
    16441 ctgagggtcg ccgagcgcga tttcaagtat tacattttcg attgggacaa caacatcctg
    16501 cacatgccca cgcgcatcca tctggagaag cgcatgccgg acggctcatg gcagccgcac
    16561 agcgtttcga cagcggcgtt tgccgtgctg cggaaggatc cggagcgcta ccgtccgccc
    16621 ggaggggtgt ggccgcgcgc gtttgtggag tttcaggatg cgcacggcga aggcgaaagc
    16681 cggttcttgc gcgacacgcg ggatgcgttg cggcgcgttc tttccggaga gatcaagccc
    16741 gggccgagtt tcgagacgtt gcgggagacg ttgcgggagg ggcgcctttt cgcgatcgtg
    16801 accgcacgcg gccatgagcc tgattcgatc cgcaaagcgg tacagttgtt catcgacatg
    16861 gtgctgacac acgaagagcg gcaagagatg atggcgaacc tgcgcgggta ccgcgcctgt
    16921 ttcgatgggc tgaccacttt cggtacggat gaggaggaat tggaatacta cctttccctg
    16981 aaccggtatc atgcggtcac ccatcccggg ttcctgcaat ggatgtctgc tgaagagggg
    17041 gacgccgggg aagccgacac cgcgagcgcc aagcagttcg cgatccgcga ttttgtcgaa
    17101 catgtgttcc ggttgttgtc gcgtacgggc gccgaatccc tgcgtcgtcc gatcagcgtg
    17161 gggttttccg atgacgaccc ggagaacgtg gcggcggttg aacggtacat tcaggaggag
    17221 ttgtcgcgcc gctttcccgc gatcaaattc tgcgtgtacg acacttcgga tccggcactg
    17281 aaagacgggc ggaaggtgac ggtcgccgga cagttggatt tcgggttctg accgcgctcc
    17341 cgactgggct tcatgcaaaa cacgctcccc gcccgcaacg acggatcagt cagatcagtc
    17401 gctccgccaa acaatccaaa cgtcgcaggt tgaagtacgc gaaaacggga acgaggacgg
    17461 cgaagcgatc ccgataggga taaaccagaa acctgaagtc cggaaccaga aaccgagttg
    17521 cgggcgaggg cgcccgcgct cccgactttt ccccatcttg atttcatgaa gggtcatatg
    17581 tttcatggta cggatgttta caggccttta cccccaaaag gatgcaactc cgctcgtgtc
    17641 acgggtgacc gctgattgac acagccgccg cagtctgcga cactgtacac ccatcatgaa
    17701 accccaacag accgttcttt ttcttggcga cggcatggcc gacgaaccga tcggcgaact
    17761 gaatgggcgt accccgcttc aagtcgcccg catcccgaac atggacacta ttgcccggaa
    17821 cgggcgcagc ggcacgatgc tcaccctgcc cgcaggattc ccgacctcca gcgatgtggc
    17881 caacatgtcg gtgctcgggt gcgacctcgc cacggagttg tgcggccgcg gaccgctcga
    17941 ggccgccagc cagggtattg cgctcggacc cgatgacatc gcctttcgca tgaaccttgt
    18001 caccgtggat gcggagggca tcctgcgcga tttctccggc ggacacatcg ctcaggcgga
    18061 tgcggtcgag tccatccgat tgctcaacga gcagttgggc ggtgaggcgg tccgttttta
    18121 tcccggtgtc agctatcgta atctgttgat cctttccggt ccgcagttca gcgccaacat
    18181 ctcgtatgac aaacctgatg accagcacgg gcaaccggtc gccaagcacc tgccgcgcac
    18241 caagtccgaa gaagggcgat tcacggccgc gctgttgcgc gacctgatcg cccgcgcggc
    18301 cgaagtgctt cagggccgta cggcgaatgg catctggccg tggagcggcg gcaaggccgg
    18361 tgcgatccat aatttctgcg aacggtacgg ggtccgcgcc gcggtgatct ctgccgtgga
    18421 tgtgatcaac gggctcggac gctgtcttgg aatggatgtg ctccccgttc ccggtgcgac
    18481 ggggtatatc gacacgaact atgagggcaa ggccgatgcc gcgctcaagg cgctcgcgga
    18541 cgggtatgat ttcgtctatg tgcatgtcga ggccatcgac gaggtttcgc acgcgcgcga
    18601 cctccaactg aaaatcaaga cgatcgagga tttcgaccgg cggctcgtgg ggcgggtcat
    18661 ggccggcgcg ggtgaccggg tcgcgtactg cgtgttgccg gatcatccgg tgccggtcgc
    18721 gaccggaaaa catacgcgcg acccggtgcc ggtcgcggtg tggcagcccg gcgtgaagcc
    18781 ggacgcggtt gcggttttcg acgaggttgc cgcgcggcgt ggcgcgttga agcatctgaa
    18841 gggtgcggac ctcatgaacc tgctgttgcg cgcgacggac ccgcagtcct gaggggaaag
    18901 ttgggcaaag ctggcggaaa tcctgacgcg ttttatatct gtgtaatcct aaaaagagca
    18961 gttgaacctt caactctctt tggtttcaag gtttctgggt ttc
//
LOCUS       DAOSRB010000032        18796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3096_length_18796_cov_6.265461, whole genome shotgun sequence.
ACCESSION   DAOSRB010000032 DAOSRB010000000
VERSION     DAOSRB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18796
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3096_length_18796_cov_6.265461"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..794)
                     /locus_tag="PLZ74_03085"
     CDS             complement(327..794)
                     /locus_tag="PLZ74_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOR97337.1"
                     /translation="MSSTSARIDRSLELFHAGCACSQAVFAAYSDLFGIDEACAMRLS
                     CGLGAGVGRLRNVCGAVSAMALLAGLKYGSETPGDAEAKRKTYEVVRQMASAFQARHG
                     SIVCRELLGTIRSETSAVPSERTTAYYATRPCARLVADAASIIEATLLVPDAP"
     gene            1063..2322
                     /gene="nusA"
                     /locus_tag="PLZ74_03090"
     CDS             1063..2322
                     /gene="nusA"
                     /locus_tag="PLZ74_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOR97338.1"
                     /translation="MNNELLSIINYLERDRGVNREIIIQAIESAIQQAARKTSDSTYE
                     LRVEVDRKTLAIKAYDTLTVSNDETGPGFISLRRAQRLRPGAKVGETVEVAVPPAQLG
                     RIAAQTARQMILQKIREAERKNMYDEYKDRIGDIVNGMVRQISHRDLIVELGKTEAIL
                     PAKERLPTEDYNIGDRIRAYVYRVQTGVNGPSVVLSRACPDFVKTLFRLEVAEIADGI
                     VEVMGVARDPGYRSKIAVKTLDEKVDPVGACVGLRGVRVRNIVRELNGEKIDIVRWSD
                     DIKQYVAQALAPARLESVSIDANELRTVNVMVAPDQLSLAIGKRGQNVRLTSKLTGWR
                     VNIIKSGEEVTFEEQRETAIASLAEVLDITTEIATTLVANGFLNTDGIIEAEIPYLQE
                     VTGLDEETVQKIWAAAQAVSGGEDREE"
     gene            2332..4899
                     /gene="infB"
                     /locus_tag="PLZ74_03095"
     CDS             2332..4899
                     /gene="infB"
                     /locus_tag="PLZ74_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOR97339.1"
                     /translation="MRVFELAKSMQTSSVEVLKQADALGIEAYSPLSALEDQEVSRLQ
                     AYFAGLGAEVKERASAERAERARAKQERARSRRMAQSQADRAVLEANRARALEMDVRA
                     RGEVSPEPAAPAPAAEAETTPPSTAAPEKPAPAPQKKTTPATAATARPTSAVKPTAAK
                     PAAAKPTAQKTKPARYASAEDELEPEEQLAASFMQRIAKEPEREMSKPPRMTPEGKTR
                     EKEKEIGRIPQRPASAASAAARPGSRPSRFTPVSQQRMESGTPPDRIILLRGAVVVKE
                     LAEKLGIRANRLIADLMQLNILASINQRVEVDVAQKVADKYGFKVEIEKAKRSTERRP
                     VLRSEDADDTIPDDKPEDLLPRPPVVTFLGHVDHGKTSLMDRIRNTRVTSGEAGGITQ
                     HIGAYTVEINGRKITFLDTPGHAAFSAMRARGASLTDIAVIIIAADDGIMPQTREAIR
                     HAQQAGVQIMIAINKCDLPTAKPDRVRQMLQSEGLTPEEWGGDIISVEVSAMTGKGVD
                     HLLEMILLQADMLELSANPARRADGFVVEAQLEQGLGPTATLLIAGGTLNVGDAILCG
                     EHFGKIRGLIDDRGRRVKSAGPATAVKCMGLSGVPEAGAAFRVMLNEKRARELAEKLA
                     QERKEIELSSAKATSLDALMSQMQENQRRELALIVKADTQGSSEAVCEALREIKSEKV
                     TLNIIHEAIGNVSATDVNKAAAGRALIVGFSVGCDAGVQQLARHAGVRINTFRIIYEL
                     LEFVKQRMLDLLPPEYKEVIRGHAEIRAIFDIGKTGRVAGCQMLDGVIRADARCRIFR
                     KKEQIWDGRFAALKHFQDDVSEVTGSQECGIFFDGFEAFQEGDTVECYVLEELPRSL"
     gene            4927..5322
                     /gene="rbfA"
                     /locus_tag="PLZ74_03100"
     CDS             4927..5322
                     /gene="rbfA"
                     /locus_tag="PLZ74_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOR97340.1"
                     /translation="MAVDRVERLNALLRREIGEALYKVFASDPLDLAAITVTKVRVAR
                     NLRNATVQVSIFGHESERGEFIRKLAGKAKELQALINRNLNLKYTPRLRFELDGSLEK
                     GDRVLDVLSRLEGLETHGETNPAPDVPPG"
     gene            5345..6085
                     /gene="truB"
                     /locus_tag="PLZ74_03105"
     CDS             5345..6085
                     /gene="truB"
                     /locus_tag="PLZ74_03105"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOR97341.1"
                     /translation="MTRYSNAPSSLTPFDGLLLVDKPSGYTSHDLVRLIRKTFRVEKV
                     GHGGTLDPGATGLLLILLGKGTKCSDQVMAGDKTYSAVIRLGRTTSTQDREGETLEEK
                     PWDTVTREAFEAKLTEFTGDLFQTPPMVSAIKVNGVPLYKLARKGQEVERKPRFVHVY
                     RFNLTEWTPPLAHVEVVCTKGTYVRTLAHDVGQALGCGACLDALRRTASGPFRVEDAL
                     PLDAVLRLTPALLAERVIPLYRLAGRRA"
     gene            6082..7083
                     /gene="ribF"
                     /locus_tag="PLZ74_03110"
     CDS             6082..7083
                     /gene="ribF"
                     /locus_tag="PLZ74_03110"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOR97342.1"
                     /translation="MRILRQAVSFRRIPGPVFLAVGFFDGVHKGHQLVLGGTVERARE
                     VGGQAWVLTFDRHPMAVLAPSKCPPLLCTLEERLHRLELSGVDGVLLLPFTRKLAVRK
                     PETFIRWLCGAPPQTGTGQNAPSPGTRLREIRCGDNWRFGRRAAGTPEELARYGGIYG
                     YRVVIVPYARYQGVEISSTRIRYAVREGRLKDAAAMLGRPFSIRDVVVRGRGLGRDRC
                     VATANLHPRSDVLPPNGVYAVRVLTGDAAYNGVANLGMHPTFTDVRPERPVLETHLLG
                     FEGDLYGQTIEVVFLERLRDECVFDSAEALASQIAEDIRNATLIFQQHGTPGPQRSP"
     gene            complement(7075..7857)
                     /locus_tag="PLZ74_03115"
     CDS             complement(7075..7857)
                     /locus_tag="PLZ74_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOR97343.1"
                     /translation="MSTQKPLIRVGLFGFGRTGAVVAAEMVDSPRCTLSWVVRKSTSR
                     MGEYVSRLHGVDSDEGMIHWIGNVNDEFFVNNPVDVIVDFSTSETVHLYRSAAQQGTR
                     ILSAISNYNREDLTELKKLSHKTAVLYSPNISIGVNLLLLTAMVVSNVIPQADVAILE
                     EHFSEKPDVSGTALRIAKKLKLDPATCVKSIRAGGIVGKHEVLFGLPNQTLRICHESI
                     TRSAFGRGALTGAEWLMECPKGFYTMEQLVASRFRDALSELR"
     gene            7957..8424
                     /locus_tag="PLZ74_03120"
     CDS             7957..8424
                     /locus_tag="PLZ74_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97344.1"
                     /translation="MKEVSRRQTLAALGAGTCACGWNAGCATLSKKGNAPEIPAEAYT
                     LEGRAVTIRLNRVPSLAAVGGSVKILDARLPQPMLIGRTGENAYAAVSLLCPHRGVEV
                     EYRHADALFRCASLGHSKFHIDGKLKKGFAKTGLTRYEAALDPADPSYLRITF"
     gene            8438..10135
                     /locus_tag="PLZ74_03125"
     CDS             8438..10135
                     /locus_tag="PLZ74_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97345.1"
                     /translation="MRKQALFAVVGMMPVLASGWGAGHDTVARALALRMPEPWCTHLQ
                     GEVLRTFCADSHYPDSFLKLSEDPRITPEEKAFLEGCNPKMTVRYHFHSDTGRGVAFT
                     LMVRALRENRRESALLWFAILGHSTADMVACNHDPIAHLATYAWGDKGWGLRFPDGTP
                     MAAMQSGLDLGWVEKEPAAKAVWDARVAKMSATDSGKSADEALLDVMRAGLDGVGVCS
                     AHGLPIVEAAAEWTMTRKAESCQELAKRLSDLGCWAVERLLRDFLTAQRLAAGDSDPS
                     MTEAVRQRFNVWFDEFVANRNYNEDSFVKGLTTAVRPDAPLLGVLSEPTWRMNEGMLG
                     PNDRVLAAQAATSLRKRGRNAVLLDVRRVMAEGISAERVPLLLVFAQKTSPYHGMDPK
                     VLVRRLIAYRAAGGKLIWIGGSVPNPALCGFPQELVAQADVGKGYAYALTRLPVGTNA
                     YAHLSLKIGDRAAYPLSRSPNFSAGWHVPSNKTFFKSGADEHVLPIAHLLDRDRVLLV
                     GAAWPRNQPAVAYLPVYVLFPYLWTDESPVFTPFEIGLDSVGNEALEAAFAALQVAP"
     gene            10237..10758
                     /gene="ispF"
                     /locus_tag="PLZ74_03130"
     CDS             10237..10758
                     /gene="ispF"
                     /locus_tag="PLZ74_03130"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOR97346.1"
                     /translation="MLRTGIGYDSHRLTDHRRLVLGGVEIPHSRGLIGHSDADVLIHA
                     LCDALLGAAAEGDIGQMFPDTDPRWENADSAVFLTAVIERLDHKGWTVVNTDATVITE
                     APKLQPHLPAMRARLAALMGIEADRVSVKAKTNERMGAVGRHEGISVLALATISRRAT
                     TGSSRRNERGDES"
     gene            10755..12200
                     /gene="cysS"
                     /locus_tag="PLZ74_03135"
     CDS             10755..12200
                     /gene="cysS"
                     /locus_tag="PLZ74_03135"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013182431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOR97347.1"
                     /translation="MMQVYNSLSRSKEPFTPLNPETVGLYTCGPTVYNFAHIGNFRAY
                     TFEDLLRRTLIFNGYRVMQVMNLTDVDDKTIRGARQAGVPLKTFTAPFIQAFFEDLAT
                     LNIQRAEVYPAATDHLPEMLDLIGKLMERGLAYRSDDGSVYFSVSAWPAYGKLARLDR
                     SALRAGARVSHDEYEKESYGDFALWKGWDEQDGEVAWDSPWGRGRPGWHIECSAMAMR
                     YLGETFDLHTGGIDNLFPHHENEIAQAEGATGKPFVAYWMHCAHLRVNGEKMSKSLGN
                     FFTLRDLLSRGWAGREIRYVLLNGHYRQPLNFTFEALDAARVSLARIDECVDALTAAA
                     GGSAPADALPDWAASARVAFTEAVNDDLNLPEALAALFALVRESNTAMRDAALTPQGA
                     AAVLDLFDRLDTVLGVIRFGRADRDSEMPPEVAERVRARAEARAAKDWSAADRLRDEL
                     ASLGWEVRDSKEGQKVKRNETMTRSTAVTAS"
     gene            12197..12886
                     /gene="tmk"
                     /locus_tag="PLZ74_03140"
     CDS             12197..12886
                     /gene="tmk"
                     /locus_tag="PLZ74_03140"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOR97348.1"
                     /translation="MTQNGNTRLQGKFITFEGPEGGGKSTHIRLLETFLRARGLDVTV
                     TREPGGTPTGETIRALLQHDQAGESPVPRTEVLLFLASRAQHVDRLIRPALARGEWVL
                     CDRFEVSTFAYQGYARGFDLDMLRRLNTFATGGLTPDLTLLLDVPPGVGGARLRDRHA
                     RNAAAGLDRFEREAKPFHEQVRRGFLDLARLAPDRIAVVNTDEETDRVAERIRETVTR
                     CLLAGESPDGC"
     gene            12876..13847
                     /locus_tag="PLZ74_03145"
     CDS             12876..13847
                     /locus_tag="PLZ74_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOR97349.1"
                     /translation="MDADAKFAMVHRAIEIGHPAHGYLIVGPVRGAALTFAERILQTL
                     FCRETVRPCGACEGCRRVTTRLEPDIHWIFPEKKSRIISVEQIRENLIREVTQTALSG
                     GWKAGVLVGADRMNDASANTFLKTLEEPPEKTIFLLLCENPQSLLPTVVSRCQRIDLD
                     LFRDLEEPWKSRVLEILASPLWGQTLENLALSSALAAILGEIKEEAERRVREETKQSA
                     AMVEEDDDVLEARVSSRYREMRADFQLTLLRWYRDLMVLRAGGDPGVVFHKPWLEVLR
                     ERAARLPLDRTFANLGFIEDSVRQMERSIPDDTVLAYTLDRIDHGVV"
     gene            13858..14766
                     /gene="ricT"
                     /locus_tag="PLZ74_03150"
     CDS             13858..14766
                     /gene="ricT"
                     /locus_tag="PLZ74_03150"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOR97350.1"
                     /translation="MLTLARIRLPENSVYQCRVPASVTVVPGEVCLAELEYGLDYGTV
                     LDLTGHTELGEGEGRGPAFRIVRKQGAEDVSRLNENAQLAEKATQAFQLSVSREKGHV
                     KLLHTRFSYGRDRFFIRYTAQIPVDLRRFVGQIQRDFKTHVDLWQVGIRDETALLGCL
                     GSCGRAACCCTWQKQFEPVNVLMAKSQEMSLNPVTLNGTCGRLKCCLRFEYEQYCEYG
                     ECLPPLRSVVTFPAADETRTDVEEEGQVIGRDILRGRLTVRTRDGRHMTVSAKAVTAK
                     RNREREYGTGKEDDNDANSRGEWTES"
     gene            14732..15190
                     /gene="aroQ"
                     /locus_tag="PLZ74_03155"
     CDS             14732..15190
                     /gene="aroQ"
                     /locus_tag="PLZ74_03155"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOR97351.1"
                     /translation="MRILVVNGPNLSRLGMREPAWYGRETLEQIMDRVRALAAERHVE
                     VEDMQSDSEGELVAVIGRARERFDGVVINPAAYTHTSVALRDAIAACGLPVVEVHLTN
                     THRREPFRHASLTVPVCLGQVMGFGGTGYLLALEGLLRHLQTEKEKESNT"
     gene            15190..16113
                     /locus_tag="PLZ74_03160"
     CDS             15190..16113
                     /locus_tag="PLZ74_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOR97352.1"
                     /translation="MARKQDAVRLCVVGSIGLDDIRTRTADRKNLLGGSCPYSTVAAS
                     FYTRAGAVGVVGSDFPKSFDRRWSAFGVDLTGVQRQNGTTFRWACEYDADMIERTTLK
                     TELGVFAAFQPVLPAAYQRAPFLLLGNIQPELQEQVLDQAKGARFVALDTMNLWIEIA
                     RPALIRVIRRANLLTVNDGEARQLMGKWNLIECAAALLKMGPSYIVIKKGEHGAMLFS
                     KRGIFLVPAYPVRRLADPTGAGDSYAGAFMGYLARAGRVSERLLREALFHASVMASFG
                     VEGFSFTRFERLDHKTIDARVRELRNMTATS"
     gene            complement(16237..17301)
                     /locus_tag="PLZ74_03165"
     CDS             complement(16237..17301)
                     /locus_tag="PLZ74_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97353.1"
                     /translation="MDRKENGHVWGVGIVGTGLIAHFHARAVAETPGARLVAVCGRNA
                     ARTAAFASTYGCEAVSSLEDLVCRSELDVLMIATPSGAHLDAAIAAARAGKHVLCEKP
                     LEISRARIDTMIHAHREAGTRLGCIFQLRYMSALEPIRAALREGRFGTLTYAGAYVPW
                     WRDAAYYHDSSWRGTWKGDGGGALMNQAIHMIDLLCDLLPPVISVSAHVSAVGHPGIE
                     TEDSAVASLGFEGGAVGVVYGTTAAWPGQAKRLEISGTDGTAVLTDDRLTRFSFREAR
                     DSDSSWVSHAPCETAAGAAQPGAMSHALHAACLGAFLESLQNGTPFAICGESARRPVA
                     LIEAIYEAARTGQTVRPNPD"
     gene            complement(17415..>18796)
                     /locus_tag="PLZ74_03170"
     CDS             complement(17415..>18796)
                     /locus_tag="PLZ74_03170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOR97354.1"
                     /translation="ADGTRNAGRCAMNARQAGRRSVLGRLGWLVVLGLGLVSPTGCVT
                     NPITGKQQISLVGKSELISLSQQAVPSQFSSDYGVVSDAQLNAYVAQVGRRLVATLKP
                     SDVVFPDMPFSFQVVNAVYVNAYAFPDGTIAITRGMLAELENEAQLAAVLGHEIAHVN
                     CGHTASAMSKGTVYDALVSGTQGYLASRGSAWTDLAGTAGQLGSAVVLASYSREQERQ
                     ADQGGMLYLVRAGYDPQGMVDLMRLLIRISGSNPSMIEQMFSSHPMSTERLETAETRL
                     REEYAGQNVGVVNQAAFLAATASIRKNRAAFQHFAAAEALLAQKQAAAAKTRAEQGLR
                     IAPNDYVGLMLVAQAERQSGQIGAAQQTALRAVRLYPAGARAHGMLAQCALQKKEYAA
                     AVAHLQVFERQVPGEPHTSFYKGLAYEGMGRKPQAAQAYRAFVRRSGTQSRAAQYATQ
                     RLQHLTGMP"
BASE COUNT         3755 a         5352 c         6044 g         3645 t
ORIGIN      
        1 ggtttcaagg tttcagggtt tcatcggggt cgggatcgct atcgggatcg ggatcgattg
       61 tttcaccaca gaacactttc ttgggtttca gggtttcatg gtttcagggt ttcatccgaa
      121 tcggggtcgg gatcgctatc gggatcggga tcgatcattt aaccacagga cacaaccgcc
      181 tcatagccta accgcctcac agtctcacag cctttgagat tgcgggctcc gcccgcatct
      241 caatacggtg cggcggggac cgcgacaaaa cccaatgttt acggggcttt gccagaattc
      301 tacccgttca acagcttctt ttgcgtttag ggagcgtccg gcacaagcaa tgtggcctcg
      361 atgatcgacg ccgcatcggc caccagacgt gcgcatgggc gcgtggcgta ataggcggtg
      421 gtgcgttcgg aagggactgc cgaggtttcg gagcgtatcg tccccagcag ttcgcggcag
      481 acaatggaac cgtggcgcgc ctgaaacgcg gacgccatct gccgcaccac ttcgtaagtc
      541 ttgcgtttgg cctccgcatc gccgggtgtt tcgcttccgt atttcagtcc ggccagaagt
      601 gccatagcag aaaccgcccc gcagacatta cgcaggcgtc ccacaccggc ccccaatccg
      661 caagacaggc gcatggcgca tgcttcgtcg ataccgaaca ggtcgctgta agcggcgaaa
      721 acagcctgcg agcaggcgca tccggcatga aacagttcga gcgaccggtc aattcgtgca
      781 gaggttgatg acatgttcac actccaattc gcaaacgggt ttaccgctat ggaccgcacc
      841 tcgtccgcat aatctggaca gcgcattgga aacatataaa ctatagtgaa acaaagaggg
      901 gttgaccatc ttgaaaagaa gccgatgcgg cggggtattg tgcattgaca tcttttgccc
      961 gaatacggta agattactta actttttgag ataaaaaggg tgggggggct taccccgctc
     1021 ttttttgttg gttgaacctt gaaagcggaa ggcggtgcgg cgatgaacaa tgagttgctt
     1081 tcgattatca attatctgga gcgagatcgc ggggtcaacc gggaaattat tatccaagcc
     1141 atcgaatccg cgatccagca ggccgcccgc aaaacatcgg actcaacgta tgaattacgt
     1201 gtggaggtcg accggaaaac cttggccatc aaagcctatg acacgctcac tgtttcgaat
     1261 gacgagaccg ggcctgggtt catctcgctg cggcgggcgc agaggctccg tccgggagcc
     1321 aaggttggcg aaaccgtgga agtcgccgtg ccgccggccc agttgggacg gatcgccgcg
     1381 caaaccgcgc gccagatgat cctgcagaag atccgcgaag ccgaacggaa gaacatgtac
     1441 gacgaataca aagaccggat cggcgacatt gtcaacggga tggtccggca gatttcccat
     1501 cgcgacttga ttgttgaact gggcaagacg gaggcgattc tgccggccaa ggagcgtctt
     1561 cccacggaag actacaacat cggtgaccgg atccgcgcgt acgtgtaccg tgtgcagacc
     1621 ggggtcaacg ggccgtcggt agtgctgtcg cgtgcctgcc cggatttcgt aaaaaccctt
     1681 ttccgactcg aagtcgcaga gatcgccgac gggattgtcg aggtgatggg cgtagcacgc
     1741 gaccccggat accgctctaa gatcgccgtg aaaacgctgg acgaaaaggt ggatcccgtt
     1801 ggcgcatgtg tcggcttgcg gggtgtgcgc gtacggaaca tcgtgcggga actgaacggg
     1861 gaaaagatcg atatcgtccg atggagcgat gacatcaagc agtatgtcgc gcaggcgttg
     1921 gcgccggccc gtctggagag tgtctcaatc gacgccaacg agttgcgcac tgtgaatgtg
     1981 atggtggcgc cggaccagct ttcactggcg atcggcaagc gcggacaaaa cgtgcggctg
     2041 acttctaagc tcaccgggtg gcgggtcaat atcataaaga gcggggaaga ggtcacgttt
     2101 gaagagcagc gtgaaaccgc gatcgcgtcg ctggcagagg tgctggacat cacgacggaa
     2161 atcgcgacca cgctggtggc gaacggattc cttaacaccg acgggatcat cgaggccgag
     2221 attccctatc tgcaggaagt gactgggctt gatgaggaaa cggtgcaaaa aatctgggcg
     2281 gcggcccaag cggtcagcgg cggcgaggac agggaagagt aaggggtgac gatgagagta
     2341 tttgaattgg caaagagcat gcagacttcg agtgtcgaag tgctgaagca ggcggatgcg
     2401 ttaggcatcg aggcctattc gcccctgtcc gcgctggaag accaggaggt gtcgcgcctt
     2461 caggcttatt ttgcgggtct gggtgccgag gtgaaggagc gtgcgagtgc cgaacgcgcc
     2521 gaacgcgcac gcgccaaaca ggagcgggca cggtcacgac gcatggcgca gagtcaggcg
     2581 gaccgggcgg tgttggaggc gaaccgtgcg cgcgccctgg aaatggacgt gcgggcccgg
     2641 ggcgaagtgt cgcccgaacc ggcggctccg gctccggctg cggaggccga gacaaccccg
     2701 ccgtccacag ccgctccgga gaaaccggcg ccggcaccgc agaagaagac cactcccgca
     2761 accgcggcaa ccgctcgccc gacatccgcc gtcaagccga ccgccgcaaa gccagccgcc
     2821 gccaagccga ccgctcagaa aacgaagccc gcgcgatatg cgtcagcgga agatgagttg
     2881 gagcctgagg agcagttggc cgcatccttc atgcaacgga tcgcgaagga gccggaacgc
     2941 gagatgtcga agcctccccg catgacgccc gagggaaaaa cgcgtgaaaa agaaaaagag
     3001 atcggacgga tcccgcagcg tccggcttcc gccgcatcgg ctgccgcgcg tcccggtagc
     3061 cgtccgtcgc gtttcacgcc ggtgtcacaa caacgtatgg aatccggtac cccgcccgac
     3121 cgcatcattc tgttgcgcgg cgcggtggtg gtgaaggaac tggcggaaaa actcgggatc
     3181 cgggctaacc ggctgatcgc ggatctgatg caactcaata ttctcgcgtc catcaatcaa
     3241 cgcgtggagg ttgatgtggc gcagaaggtc gctgataaat acggcttcaa agtcgagatt
     3301 gaaaaagcca agcgctcaac ggagcgcagg cccgtactgc gcagcgagga tgcggatgac
     3361 accatcccgg acgacaaacc cgaggatttg ttgccgcggc cgccggtggt caccttcctc
     3421 ggccatgtcg accatggcaa aacatcgctg atggaccgga tccgtaacac gcgcgtcacc
     3481 agcggcgagg cgggggggat cacccaacac atcggtgcct atacggtgga aatcaatggt
     3541 cggaaaatca cctttctgga tacgcccggc catgccgcct tttccgcaat gcgcgctcgc
     3601 ggcgccagtc tgacggatat cgcggtgatc atcattgcgg cggatgacgg catcatgccg
     3661 cagacccgtg aggcgatcag gcatgcccag caggcggggg tacagatcat gatcgcgatc
     3721 aacaaatgcg acctgcccac cgccaagccc gaccgggtgc gccagatgct gcaaagcgag
     3781 ggcctgactc ctgaagagtg gggcggcgat atcatcagcg tggaagtctc ggccatgaca
     3841 ggcaagggcg tggaccatct gcttgaaatg atcctgctgc aggcggacat gctcgaattg
     3901 agcgccaatc cggcgcgccg cgcggacggg ttcgtggtgg aggcccagct cgaacagggg
     3961 ctcggaccaa ccgccaccct gctgattgcg ggaggcacgc tgaatgtggg agacgcgatt
     4021 ttgtgcgggg agcatttcgg caagatccgc gggttgatcg acgaccgcgg acgccgcgtg
     4081 aagtcggcgg gccccgccac ggcggtcaag tgcatgggac tgtcgggtgt gccggaggcc
     4141 ggggcggcgt tccgcgtgat gctcaatgag aaacgtgccc gcgaactggc ggagaaactc
     4201 gcccaagagc ggaaggagat cgagctgtcc agcgccaaag ccacctcgct ggatgcgctg
     4261 atgagtcaga tgcaagagaa ccagcgccgc gagttggcgc tgatcgtgaa ggcggatacc
     4321 cagggttcct ctgaggccgt ttgcgaggcg cttcgcgaaa tcaagagcga aaaggtcacg
     4381 ttgaacatca tccacgaggc catcgggaat gtgtctgcca cggatgtgaa caaagccgcg
     4441 gccggtcgcg cgctgattgt gggcttcagt gtcgggtgcg atgccggtgt gcagcaactc
     4501 gcccgtcatg ccggagtgcg catcaatacc ttccggatca tctatgaact gcttgaattc
     4561 gtgaagcagc gcatgcttga tctgctgccg cccgaatata aggaagttat tcggggccat
     4621 gccgagattc gcgctatttt cgatatcggc aagaccggga gggtggcggg ttgccaaatg
     4681 ctcgacggtg tgattcgcgc ggatgcgcgg tgccggattt tccgcaagaa agagcaaatc
     4741 tgggatggcc gtttcgccgc cctcaaacac ttccaggacg acgtttccga agtcaccggt
     4801 tcgcaggagt gcggtatttt cttcgacggg ttcgaggcgt ttcaggaggg cgacacggtc
     4861 gagtgttacg ttctggaaga gttgccgcgc agcctatgag gtgcgctgcg gcgcaaggag
     4921 gaggcgatgg ccgttgatcg ggtagaaagg ctgaacgccc tgttgcggcg tgagatcggc
     4981 gaagcgctgt acaaagtgtt tgccagcgac ccgctggatt tggccgcgat cacggtgacg
     5041 aaggtgcgcg tcgcgcgtaa tctccgcaac gcgaccgtgc aggtctcgat tttcggccat
     5101 gaaagtgaac gcggggagtt catccggaaa ctcgccggga aagccaagga actgcaagcc
     5161 ctgatcaacc gcaatctgaa cctgaagtac acaccccgtc tccgctttga attggacggt
     5221 tcgctggaaa agggggatcg cgttctggat gtgctgagtc ggctggaggg gttagaaacg
     5281 cacggcgaaa cgaaccccgc tcccgacgtc ccgcccggct gagccgagag gaaaacccgt
     5341 tgagatgaca cgttattcga acgcgccctc ctccctgacc ccttttgacg gtcttctgct
     5401 ggtggataaa ccgtcggggt acacttcgca tgatctggta cgcctcatcc ggaaaacgtt
     5461 tcgtgtggag aaggtggggc atggaggcac gttggacccc ggcgccacgg gcttgctctt
     5521 gattctgctg ggcaagggta cgaaatgctc ggatcaggtc atggctgggg acaagaccta
     5581 ttccgccgtg atacggttgg gccggaccac ctccacgcag gacagggaag gcgaaacgct
     5641 tgaggaaaaa ccttgggata cggtgacgcg tgaagcgttc gaagcgaagt taaccgaatt
     5701 cacgggggat ttgttccaga cgccgcccat ggtttcagcg atcaaagtga acggtgtgcc
     5761 gctctacaaa ctggcgcgta aagggcagga ggttgagcgt aaaccccgct ttgtgcacgt
     5821 ctaccgtttc aacctgaccg aatggacccc gccgctggcg catgtggagg tggtctgcac
     5881 caaaggcacc tatgtgcgca cattggccca tgatgtgggc caggccttgg gctgcggcgc
     5941 gtgtctcgac gcgctgcgac gcaccgcttc cggtcccttc cgggttgagg atgcgctgcc
     6001 gttggatgcg gttctgcggc tcacgcctgc gcttctcgcc gaacgggtga ttcccctgta
     6061 ccgcctcgca gggagacgcg catgagaatc ctacgccaag ccgtttcttt ccggcggatt
     6121 ccgggaccgg tttttctggc cgtcggattt tttgacgggg tgcacaaggg acatcagctt
     6181 gtgttgggag gcacggtgga acgggcccgc gaagtcggcg ggcaggcatg ggtcctgacc
     6241 ttcgaccgtc atccgatggc ggtgctcgcg ccctcgaagt gccccccgct gctgtgcaca
     6301 ctggaggagc gtctgcaccg gctggaactg tccggagtgg acggggtcct gctgttgccg
     6361 ttcacccgga aacttgcggt ccggaagccg gaaaccttta tccgctggtt gtgcggggcg
     6421 cctccgcaaa cgggcacagg tcagaacgct ccctcgccag gcacacgact gcgtgagatc
     6481 cgctgcggcg ataactggcg tttcggccga cgcgctgcag gcacgccgga ggagctcgcc
     6541 cggtacggcg ggatctacgg gtaccgtgtg gtgatcgtac cgtatgcccg ctatcaggga
     6601 gtggagattt ccagcacgcg gattcggtat gccgtgcggg aagggcgttt gaaggacgcg
     6661 gcggcaatgc ttggacgccc cttttcgatt cgggatgtgg tggtgcgcgg acgcgggctc
     6721 ggccgggacc ggtgtgtggc aacggccaac ctgcatcctc gctcggatgt gctgccgccc
     6781 aacggagtct atgccgtgcg cgtactgacg ggcgatgccg cctataacgg tgtggcgaat
     6841 ctgggaatgc acccgacctt cacggatgtg cgccccgaac gccctgtgtt ggaaacgcac
     6901 ctgcttgggt tcgaagggga tttgtacggc caaacgatcg aggtcgtgtt tctggagcgg
     6961 ttgcgcgatg agtgcgtgtt cgattcggct gaagccctcg cgagtcagat cgcggaagat
     7021 atccggaacg ccaccctaat ttttcaacaa cacggaacgc ccggtccgca gcggtcaccg
     7081 tagttccgac agcgcgtccc ggaagcggct ggccaccaac tgctccatcg tgtaaaaccc
     7141 cttcgggcac tccatcagcc attcggcacc ggtcagcgcg ccgcgcccga aagcagagcg
     7201 ggtgatgctt tcatgacaga tgcgcaacgt ctggttgggc agcccgaata aaacctcgtg
     7261 cttcccgaca atcccccccg cgcggatgga tttgacgcaa gtggcgggat cgagcttcag
     7321 ttttttcgcg atccgcaagg cggtccccga cacgtccggt ttttcgctga agtgttcttc
     7381 aagaatcgcg acatcagcct gtgggatcac gttgctgacc accatggctg tcaacagcaa
     7441 cagattcacg ccgatggaga tgttcggcga gtagagcact gcggttttat gcgatagttt
     7501 tttgagttcc gtcaggtctt ctcgattgta gttggaaatc gcggaaagaa tacgtgtgcc
     7561 ttgctgggcc gcgcttcggt agaggtggac ggtttcggaa gtggaaaaat ccacaatcac
     7621 gtccaccggg ttgttcacga aaaactcgtc gttcacattg ccaatccagt ggatcatccc
     7681 ttcatccgaa tccactccgt gcaaccggct cacgtattcc cccatgcggc tggtgctttt
     7741 gcgcaccacc caggaaagcg tgcagcgggg ggagtcgacc atttctgcgg cgaccacggc
     7801 gcccgtgcgc ccgaaaccga acaacccgac tcgaattaag ggtttttgtg tgctcatcaa
     7861 gagggtttta taatcgaata tgcgtgttcc gtcaaaggaa atcgggtaag atgaagatgt
     7921 gaaatgacgt gtgctgactt gaaaaagggg ggagagatga aagaggtgtc tcgccgtcag
     7981 actttggcgg ctctgggtgc gggaacgtgc gcttgcggct ggaatgccgg atgtgccacc
     8041 ctgtcgaaaa aaggaaatgc gccggagatc ccggcggaag cctacaccct tgaaggccgt
     8101 gcggtcacga tacggttgaa ccgggtgcca agcctcgccg ctgtgggcgg atcggtcaaa
     8161 atcctcgacg cgcgcctgcc gcaaccgatg ctgatcggac ggaccggcga aaacgcgtat
     8221 gcggcggttt cgctgctctg cccgcatcgc ggcgtggagg tggaataccg acatgcggac
     8281 gcgcttttcc gttgcgcgag tctgggtcat tcgaaattcc acatcgacgg caagctcaag
     8341 aaaggctttg cgaaaaccgg attgacccga tatgaggcgg ccttggatcc ggcggatccg
     8401 tcttacctcc ggatcacgtt ctaaaatggg gagagagatg aggaaacaag cgctgttcgc
     8461 ggtggtgggg atgatgccgg tgctggcctc cgggtggggt gcggggcacg acacggtggc
     8521 gcgcgcgctt gccctgcgta tgccggagcc gtggtgtacg cacttgcaag gtgaggtgct
     8581 gcggacattc tgtgccgaca gtcattatcc ggattcgttt ctcaagttga gcgaggatcc
     8641 gcggatcaca ccggaggaga aagcgtttct ggagggttgc aacccaaaga tgacggtacg
     8701 gtatcatttt cattcggaca ccggacgtgg tgtggccttt acgctgatgg tgcgcgcgtt
     8761 gcgcgaaaac cgccgggaga gcgcgttgtt gtggttcgcc attctgggac attcgactgc
     8821 ggatatggtg gcctgcaacc atgaccccat tgcgcatctg gcgacgtacg catggggaga
     8881 caaggggtgg gggctccgtt ttccggacgg gacgcctatg gccgcgatgc agtcgggcct
     8941 tgatctcggc tgggtggaga aagaacccgc cgcgaaagcg gtgtgggacg cgcgcgtggc
     9001 gaaaatgtcc gcgaccgatt ccggcaagag tgcggatgag gcgcttctgg atgtgatgcg
     9061 ggccggactc gatggggtag gggtctgttc cgcacacggg ctgccgattg tcgaggcggc
     9121 ggccgagtgg acgatgacgc gaaaagccga gtcctgtcag gagcttgcga aacgtctttc
     9181 agatctgggt tgttgggcgg tggagcggct gttgcgtgac tttctcacgg cacagcggct
     9241 ggctgcgggc gatagcgatc cctcgatgac ggaggcggtg cggcagcggt tcaacgtctg
     9301 gtttgacgag tttgtggcta accggaacta taatgaggac tccttcgtga aaggattgac
     9361 cacagcggtc aggcccgacg cgccgctgtt gggcgtgctg tcggaaccga cttggcgcat
     9421 gaacgagggg atgctggggc cgaacgaccg cgtgctggcc gcgcaagccg ccacgtcgct
     9481 gcgcaagcgc ggcaggaacg ccgtcctgct ggatgtgcga cgggtgatgg cggaggggat
     9541 atccgcagaa cgggtgccgc tgttattggt tttcgctcaa aagacttctc cttaccatgg
     9601 gatggatccc aaggttctcg tccgccgcct gattgcgtat cgcgcggcgg gaggcaaact
     9661 gatatggatc ggcggcagcg tgccaaatcc agcactctgc ggtttcccgc aagagcttgt
     9721 tgcgcaggct gatgtcggca aagggtatgc ctacgccttg acgcggctgc cggtcggaac
     9781 gaacgcctac gcgcacctct ccctgaagat cggtgatcgg gcggcgtacc ctctgtcacg
     9841 atcgccgaat ttcagcgcgg ggtggcatgt gcctagcaac aaaacctttt tcaaatccgg
     9901 ggctgatgag catgtcctgc cgattgccca tttgttagac cgcgaccgcg tattgctggt
     9961 gggtgccgca tggccccgaa atcaacctgc tgtcgcgtat cttccggtgt acgtgctctt
    10021 cccgtacttg tggacggacg agtctcccgt gttcacgccg tttgagatcg ggctcgactc
    10081 ggtggggaac gaagcgttgg aagccgcttt cgcggcgctg caagtcgcgc cttaagcttt
    10141 ataatctgcg taatctgtgg accaaatgat tccatggttt attctcgctc cgcctttcgg
    10201 tttgtcccac agcagggttt ccggtatagt gtcgccatgc tacgtaccgg aataggctat
    10261 gactcacacc ggctgacgga ccaccgccgt ctggtgctgg gcggagtcga gatcccgcat
    10321 tctcgggggc tgattggaca ctcggatgcc gatgtgctga tccacgcgtt gtgcgacgca
    10381 ctgttgggtg cggccgcgga gggtgacatc ggccagatgt ttccggacac ggatccccgt
    10441 tgggaaaatg ccgacagcgc ggtgtttcta acagccgtga tcgaacgtct tgaccacaag
    10501 ggatggactg tcgtgaacac ggatgccacc gtcatcacgg aagccccgaa actgcaaccg
    10561 catcttcccg ccatgcgcgc gcggctcgcc gcgctcatgg ggattgaagc agaccgggtg
    10621 tcagtcaaag ccaaaacgaa tgagcgcatg ggggcggtgg ggcgtcacga ggggatcagt
    10681 gtgctggccc tcgccacgat ttcccggcgc gcgaccaccg gttcaagcag gcggaacgaa
    10741 cgcggagatg aatcatgatg caggtgtaca acagtctgtc acggagcaag gagcctttta
    10801 ccccgctgaa ccccgagacc gtagggctct acacatgcgg tcccacggtc tacaactttg
    10861 cccatatcgg caatttccgc gcgtatacgt ttgaagatct gttgcgccgc acactgattt
    10921 tcaacgggta tcgggtcatg caggtcatga acctcacgga tgtcgatgac aaaaccatcc
    10981 gcggcgcacg gcaggccggc gtgccgctga agacattcac ggcaccgttt atccaggcct
    11041 tttttgagga tctcgcgacc ctgaacatcc agcgcgccga agtctatcct gcggcgaccg
    11101 atcatctgcc cgaaatgctg gacctcatcg ggaaactgat ggagcgtggc ctggcgtacc
    11161 gatccgacga cggctcggtt tatttcagtg tcagcgcctg gcctgcatac gggaagctcg
    11221 cgcgtctgga ccgttccgcc ttgcgtgccg gcgcgcgcgt ttcgcacgat gagtacgaaa
    11281 aggagagcta cggcgatttc gccctttgga aaggatggga cgaacaggac ggggaggtgg
    11341 catgggattc gccgtggggc aggggacgtc cggggtggca tatcgaatgt tcggccatgg
    11401 ccatgcgcta cctcggtgag acgttcgatc tccataccgg cggcatcgac aatctctttc
    11461 cgcatcacga gaacgaaatc gcgcaagccg agggtgcgac cggcaagccg ttcgtcgcgt
    11521 attggatgca ctgcgcgcac ttgcgcgtca acggggagaa gatgtcgaaa tccctcggca
    11581 attttttcac attgcgcgat ctgttgtccc gaggttgggc cggacgggaa atccggtatg
    11641 tgctgctgaa cggacactac cggcagccgc tgaatttcac gtttgaggcg ttggacgcgg
    11701 cccgcgtatc tctggcgcgc atcgacgaat gtgtcgatgc gctgaccgcc gcagcgggcg
    11761 gctcggcacc agcggatgcg ctgccggatt gggccgcctc ggcgcgtgtg gcctttacgg
    11821 aggcagtcaa cgacgacctg aatctgcccg aggcgcttgc ggcgctgttc gcgctcgtgc
    11881 gggagagcaa caccgcgatg cgggatgcgg cgttgacgcc ccagggcgcc gccgctgtgc
    11941 tggacctgtt cgaccggctg gacacggttc ttggggtgat ccgattcgga cgcgcagacc
    12001 gggacagcga gatgccgccg gaagtcgcgg aacgggttcg cgcccgggcc gaggcgcgcg
    12061 cggcaaagga ctggagtgcg gcggaccggt tgcgtgacga acttgcgtcc ttggggtggg
    12121 aagtgcgtga ctcaaaagag ggccagaagg tgaagcggaa cgagacgatg acgcggagta
    12181 cggcggtgac ggcatcatga cgcagaacgg caatacccgg ttgcaaggga agttcatcac
    12241 gttcgagggg ccggaaggcg gcgggaaatc cacacacatc cgcctgctgg aaacatttct
    12301 gcgcgctcgc ggcctcgacg tgaccgtcac acgcgaaccg ggcggcacgc ctacgggtga
    12361 gacgatccgc gcgctgctgc aacatgatca ggcaggggag tcgccggtgc cgcgtacaga
    12421 ggtgctgctc tttctggcta gccgcgcaca gcatgtcgac cggctgatcc gtcccgccct
    12481 ggcccgtggc gaatgggtgc tctgcgaccg gtttgaggtt tccacattcg cctatcaagg
    12541 gtacgcgcgg gggttcgacc tcgacatgtt gcgtaggctg aacacgttcg ctaccggcgg
    12601 gctgacgccc gatctgaccc ttctgttgga tgtgccgccc ggagtcgggg gggctcgcct
    12661 gcgggatcgc catgcgcgaa acgccgctgc cgggcttgac cggttcgaac gcgaggcgaa
    12721 accgtttcat gaacaggtgc gtcgcggttt tctggattta gcccgcttgg cccccgaccg
    12781 gattgcggtc gtgaacacgg atgaggagac ggatcgagtg gcggagcgga tccgcgagac
    12841 cgttacgaga tgcttattgg caggggaatc gcctgatgga tgctgacgca aaattcgcga
    12901 tggtgcaccg agccatcgaa atcgggcatc cggcacacgg atacctgatc gtcgggccgg
    12961 tacgcggcgc cgccctgacg tttgctgaac gcattctgca aaccctcttc tgccgtgaaa
    13021 ccgtgcggcc gtgcggagcc tgcgaagggt gccgccgcgt gacaacgcgg ttggaacccg
    13081 atattcactg gatattccct gaaaaaaaat cccgcatcat cagcgtcgag cagatacggg
    13141 agaacttgat ccgcgaagtg acccagaccg cgctgtccgg aggatggaag gccggggtct
    13201 tggtcggggc cgaccggatg aatgatgcgt ccgccaacac gtttctcaaa acgttggagg
    13261 agcctcccga aaagacgatc ttcctcctgt tgtgcgagaa cccccagtct ctgctgccga
    13321 ctgtggtctc gcgttgccaa cggatcgatc tagacctttt ccgcgatctg gaggaaccgt
    13381 ggaaaagccg cgtgctagag atcctggcca gtccgctttg ggggcagacg ctggagaatc
    13441 tggccttgtc cagcgcactc gcggccatac ttggcgaaat aaaagaggag gcggagcggc
    13501 gggtacgcga ggaaacgaaa cagagcgcgg caatggtgga ggaagacgac gatgtcctcg
    13561 aggcccgtgt gagttcgcgt taccgcgaaa tgcgggcgga ttttcaactc acgctgcttc
    13621 gctggtatcg cgatctcatg gttttgcgtg ccggaggaga tcccggggtg gtttttcaca
    13681 agccttggct ggaggtgctg cgtgagcggg cggcccgtct gcccctggac cggacctttg
    13741 ccaatctcgg gttcattgag gattcggtgc gccagatgga gcgcagcatc ccggatgata
    13801 cggttctggc gtatacgttg gaccgcatcg atcatggcgt agtctaggaa atgccctatg
    13861 ttgactctcg cacgcatacg gttgcctgaa aacagcgttt atcaatgtcg tgtgcccgca
    13921 tcggtcacgg tggtgccggg ggaagtatgt ctggctgagt tggaatacgg actggactac
    13981 ggtacggtgc tggatctgac cggacacacc gagctaggcg agggggaggg gaggggcccc
    14041 gcctttcgca ttgtgcgcaa acagggtgcg gaagacgtgt cgcggctaaa cgagaacgcg
    14101 cagttggccg aaaaggcgac acaggcattc cagctttcgg tgagccgcga gaaagggcat
    14161 gtcaaactgt tgcacacgcg gttttcctac ggtcgggacc gttttttcat ccgctatacg
    14221 gcacaaatcc cggtggacct gcgtcggttt gtgggacaga tccagcggga tttcaaaacg
    14281 catgtggatc tgtggcaggt cggaatccgc gatgagaccg cgcttctggg ctgtttggga
    14341 tcgtgcggtc gcgccgcctg ttgctgcacg tggcagaaac agtttgagcc ggtcaacgtg
    14401 ctgatggcga aaagtcagga gatgtcgctc aatccggtca ccctcaacgg cacgtgcggc
    14461 cgtctgaaat gctgcctgcg tttcgagtac gaacagtatt gcgaatacgg ggaatgtctc
    14521 ccgccgttac gaagtgtggt gacattcccg gccgccgatg aaacgcggac ggacgttgaa
    14581 gaggaggggc aggtgatcgg ccgggatatc ctgcgcgggc ggttgacggt caggacgcgc
    14641 gacggacgac atatgaccgt gtccgcaaaa gcggtgaccg ccaagcggaa tcgagagcgg
    14701 gaatacggaa caggcaagga ggatgataac gatgcgaatt ctcgtggtga atggaccgaa
    14761 tcttagtcgg ttgggcatgc gtgaaccggc atggtacggg cgggaaaccc tcgagcaaat
    14821 catggaccgg gtgcgtgcct tggcagcgga acgtcatgtg gaagtcgaag acatgcaaag
    14881 tgattcggag ggagaactgg tggccgtgat cggtcgggca cgggaacgct tcgacggggt
    14941 ggtgatcaat cctgcggcgt acacgcatac cagcgtcgcg ttacgcgacg caatcgcggc
    15001 ctgtgggctc cccgtggttg aggttcacct gaccaacacc catcggcgcg aaccctttcg
    15061 gcatgccagt ctgacggtac cggtctgcct cggtcaggtg atggggttcg gcggcaccgg
    15121 atatctgttg gcgctggaag ggctgctgcg tcatctgcaa acggaaaagg aaaaggagtc
    15181 gaatacataa tggcacggaa acaggatgcg gttcggctgt gcgttgtcgg atcgattggc
    15241 ttggatgata tccggacacg gacggccgac cggaaaaact tgctgggcgg atcatgccct
    15301 tacagcacgg tcgccgcttc gttttatacc cgcgccggtg cggtaggggt ggtgggcagt
    15361 gattttccaa agtccttcga ccgccgctgg tccgcgttcg gcgtggatct gaccggggtc
    15421 cagcgtcaaa atggcacaac tttccggtgg gcgtgcgagt atgacgcgga tatgatcgag
    15481 cggaccacgc tgaagacgga actgggggta ttcgcggcat ttcagcccgt tttgcccgca
    15541 gcctatcaac gcgctccctt tctgctgctc ggtaacattc aacctgaact tcaagagcaa
    15601 gttttggatc aggcgaaggg tgcccggttt gtggcactgg ataccatgaa cctctggatc
    15661 gagatcgcgc gtccggcgct gatccgggtg atccggcgcg cgaacttgct gaccgtgaat
    15721 gacggggagg cgcgtcagtt gatgggaaaa tggaatctga tcgagtgtgc ggcggcattg
    15781 ctcaaaatgg gtccgtcgta tatcgtcatc aaaaaaggcg aacatggcgc gatgctcttc
    15841 tcgaagcgcg ggatatttct ggtcccggcg tatccggtgc gccgtctggc ggacccgacc
    15901 ggtgccggag attcatatgc cggcgcattt atgggttatc tggcgcgcgc cggacgtgtc
    15961 agcgaacggc ttttgcgcga ggcgcttttc cacgcgagcg tcatggcctc gttcggggtc
    16021 gagggattca gtttcacgcg gttcgaacgt ttggatcaca agacgattga cgcgcgggtc
    16081 cgtgaactcc gtaacatgac cgcaacatca taaaaattgc ggtgctgtct accggtcttg
    16141 cttcctctgt ctctgcctgc cgccaaactt gacataatat atattatgcg acgttattgt
    16201 ttgggcggtc atttacccca aaacgggctg aaatcgctag tccggattcg gccggaccgt
    16261 ctgtcctgta cgtgccgcct catagatcgc ctcaatcaga gccacaggac gacgggctga
    16321 ttcgccgcag atcgcaaagg gtgttccgtt ctggagggat tcaaggaacg cgcccaagca
    16381 tgcagcgtgc aaagcgtgcg acatggcacc gggttgggcc gcgcctgcgg ctgtctcaca
    16441 aggagcgtgt gaaacccacg aggagtccga atcgcgcgct tcgcggaagg aaaaccgtgt
    16501 caggcgatca tcggtcagga ccgccgtgcc gtcggtgccg ctgatttcaa gccgctttgc
    16561 ctgtcctggc caggctgcgg tcgtgccgta caccaccccc acggccccgc cttcaaaacc
    16621 gagcgaggcc accgcactat cttcggtttc aattccggga tggccgacag cggaaacatg
    16681 cgcgcttacc gagatgaccg ggggaagcag gtcgcacaac agatcaatca tgtggatcgc
    16741 ctgattcatc aaagcccctc ccccatcccc tttccacgtc ccgcgccaag acgaatcatg
    16801 gtaatatgcc gcatcccgcc accatggcac atatgccccg gcataggtga gcgtcccgaa
    16861 gcgcccttcg cgaagagcgg cgcgaatcgg ttccagcgca gacatgtacc ggagttggaa
    16921 aatgcatccc aggcgtgtgc cggcctcgcg atgcgcatgg atcatggtgt cgatccgagc
    16981 cctcgagatt tccagcggtt tttcgcacaa aacatgtttc ccggcgcggg ccgcggcgat
    17041 tgcggcatcc agatgcgcgc cggaaggcgt cgcgatcatc agcacatcca actcgctccg
    17101 gcaaaccaga tcctccagcg aggaaaccgc ttcacacccg tatgttgacg caaacgccgc
    17161 cgtacgcgca gcgttccgtc cgcagaccgc gaccagacgg gcaccgggcg tttccgccac
    17221 ggcccgcgca tgaaaatgcg cgatcagtcc cgttccgaca atccccaccc cccatacgtg
    17281 tccgttctct ttcctgtcca tcagacctcc acgactaacg caaaccacaa catcacccaa
    17341 cgctgctcgt ctccttccga tttcgtcgcc atcaatgcgt atcgaggatc atggggcacg
    17401 cggccggtcg ctggttacgg cattcctgtg agatgctgca accgttgcgt cgcgtattgg
    17461 gctgctctag actgcgtgcc gctgcgtctc acaaaagccc gatatgcctg cgcggcttgc
    17521 ggtttacgtc ccatgccctc ataggccaag cctttgtaaa aagaggtatg gggttcaccc
    17581 ggaacctgcc gttcgaagac ttgcagatgg gcgacagctg cggcgtactc ttttttctgc
    17641 aacgcacact gcgccagcat accgtgcgcg cgcgcgccgg caggatacag gcgaaccgcg
    17701 cggagagccg tctgctgcgc ggccccgatc tgcccgctct gtcgttcggc ctgcgcgacc
    17761 agcatcagcc ccacataatc attcggcgcg atccgcaaac cctgctcggc ccgggttttg
    17821 gctgccgcgg cctgcttttg cgcgagcaac gcctccgccg ccgcgaaatg ctggaacgcg
    17881 gcccgattct tccggatgga cgcagtggcc gcgaggaatg cggcctggtt cacgacaccg
    17941 acgttctgtc ccgcatattc ctcccgcaac cgcgtttcgg cggtttccaa ccgttccgtg
    18001 ctcatcggat gcgatgagaa catctgttcg atcatcgaag ggttggaacc gctgatgcgg
    18061 atgagcagac gcatcaggtc caccatcccc tgaggatcat aacccgcccg caccagatat
    18121 agcatgccgc cctgatccgc ctgccgctcc tgttcccggc tgtagctggc aagaaccacc
    18181 gcgctgccaa gttggcctgc ggtccccgcc aaatccgtcc acgcgctgcc gcggctcgcc
    18241 agatacccct gcgtgccgga aaccagcgcg tcatacaccg tacccttgct catggcggat
    18301 gcggtgtgcc cgcaattcac atgagcgatc tcgtgtccaa gcaccgccgc aagctgtgcc
    18361 tcgttctcca gttccgccaa catcccgcgt gtgatggcga tggtcccgtc cggaaatgcg
    18421 taagcattga catagaccgc gttcaccacc tgaaaggaga aaggcatatc cgggaaaacc
    18481 acatccgaag gcttcagcgt ggcgaccagc ctgcgtccga cctgagcgac ataagcattg
    18541 agttgagcgt cggacaccac cccgtaatcc gatgagaatt gcgacggcac ggcctgctgg
    18601 gacagagaga tcaactcact ctttcccaca aggctgatct gctgttttcc agtgatggga
    18661 ttggtcacgc atccggtcgg cgagaccaac cccaatccca gcacaaccaa ccatcccagg
    18721 cggcccagca cactgcgtct cccggcttgt ctcgcattca tcgcacacct cccggcattc
    18781 cgggttccgt ccgcat
//
LOCUS       DAOSRB010000033        18687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3122_length_18687_cov_6.079058, whole genome shotgun sequence.
ACCESSION   DAOSRB010000033 DAOSRB010000000
VERSION     DAOSRB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18687
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3122_length_18687_cov_6.079058"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..960
                     /locus_tag="PLZ74_03175"
     CDS             139..960
                     /locus_tag="PLZ74_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR97355.1"
                     /translation="MEVTFLGTGTSVGVPMIGCRCAVCTSADPRNTRRRTSLYVRTPG
                     AALVIDTPPDFRQQALDFGVDRVDAVVFTHAHADHIFGFDDIRRFNTLQQKVIPAYAD
                     TETLADVRRVFSYIGDRPSPLGLYRPLVEFIEVHGPFTVGDVQLTPLDVCHGNKMTGY
                     LLEREGCRIGYVPDCHTMPDATVRRLRGVDVMILDALRYKPHPSHICVEESLRLLSEI
                     DAGRSYLIHLCHDVDHAELEARLPEGVQVSFDGLRITVDETAAARPLPVRERSAG"
     gene            993..1802
                     /locus_tag="PLZ74_03180"
     CDS             993..1802
                     /locus_tag="PLZ74_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97356.1"
                     /translation="MAGGVMYAAAVAAQQVEVRLTLAYNGFVIGEPVLVEVELLNATR
                     NPVVVGEKGAQSALFMDVSFGDAYNLLAPVNPEPLAGAFTLNPGQTFRRKLELDKWYP
                     LVREGRYMTQLVLAHEGMRYESVKKSFDVVPGIPVCEGVQMFVNRQKLKRHFKLVYWH
                     RNQTDRLFLRTEDEPGALIWDAIDLGILLRGTTPKLDISPQGEVTVVHRSTQDAFLRT
                     VLWSLPDSLEIVERNSLMDPEISASQRVKSLYGEMAEGQTGEEKKAWWKFW"
     gene            1834..2931
                     /gene="tsaD"
                     /locus_tag="PLZ74_03185"
     CDS             1834..2931
                     /gene="tsaD"
                     /locus_tag="PLZ74_03185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOR97357.1"
                     /translation="MVVLGIETSCDETAAAVVADGRRVLSNVVYSQVPLHQPYGGVVP
                     EIASRSHVEKLPGVVAEAVRAAFAPESPAGSAPPPAAWREVDAVAVTYGPGLASSLIV
                     GLSAAKGLALRLERPLIGMNHIEAHIHSVFLNPEAPDPREAAPLLALVVSGGHSCWIE
                     TDRPGNYRMIGQTLDDAAGEAFDKAAKLLGLGYPGGPEIDRLARQAARVDAVAFPKGS
                     PRPGNPALAGLRAELCVSFSGLKTALLYHLRSHPPQDQNEVAEVAAAYQEAIVTALAE
                     RSERALRGGRYAAFAVGGGVSLNRRLRERLTEVARAAGVRLLLAEPRFCGDNAAMIAG
                     LAGAGGGVRGPAAQELDASPALAAGEEGTAW"
     gene            2925..3971
                     /locus_tag="PLZ74_03190"
     CDS             2925..3971
                     /locus_tag="PLZ74_03190"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOR97358.1"
                     /translation="MVRVTRRVLLSGVAVAWLLCSGVLAADPDPLVVASWNVENLFDT
                     EDDPANKGDDGFTPRGWMRWTAARYRLKLEHLAEIIAGMRPDVLCLMEVENRRVLEDL
                     VQTLERKHAFSLPVIVHRDAEEKRGIDVALLARAAPVATQWLRTAPGQREVVACDFSF
                     GGRRLTVLVNHWKSQFGKKGESDEIRQREARAVRAFLDERLRAEPAAAILVAGDFNDA
                     PDSPILTQDAGFVMDEARVRGDASGRLLFNLAGSLSESARVTYYYAASKKWYTMDAIS
                     VTRGMLADAVPAAPWRVRKSGYTVFKTAAQQDSQGAPIPFRRVRNKKEGDSFRTGYSD
                     HFPVRVELEPSAAP"
     gene            complement(3999..4529)
                     /locus_tag="PLZ74_03195"
     CDS             complement(3999..4529)
                     /locus_tag="PLZ74_03195"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOR97359.1"
                     /translation="MRKGKHPFAEGEPIPGESESEGYAVPDESAFLSAASRARCTPTP
                     REAARLAAVRTLFQRVKPGLPIDSDPPELRQEPTPLGHAIETILQRLELRATPWLDDL
                     AAAWHEILPPQVSRFTRPGKYDAGILFVFVTSSTHLFEIRRNHLQRIEQAVRAFAGDE
                     RQIRQVRLMVDSVPIP"
     gene            4580..5818
                     /locus_tag="PLZ74_03200"
     CDS             4580..5818
                     /locus_tag="PLZ74_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOR97360.1"
                     /translation="MKKGNMLIAQSGGPSMVINQSLVGAVLAARRSGSGVGKIYGALH
                     GIRGILDGAFIDLRKETTATLEAVAATPSSALGSVRHKPSAEDCLAVFRRLRQRDIRY
                     FFYIGGNDSAETVHILHEEAVKNECDIRCFHIPKTIDNDLCGNDHTPGYGSAARFVAC
                     AVKGDDLDNRALGGVKIDVIMGRNAGFLTAAAALARTEEDDGPHLIYLPERPFSVTRF
                     VRDIKAAMRDYGRCVVAVSEGITDRRGTPLVAAFTREKDSHGNVQLSGTGALGDLLAN
                     VVKTRTGITRVRADTFGYLQRSFPGVVSPTDALEARKVGEEAVLAAVGKNMASGSIAI
                     RRKPGKRYGVRFEVVPLHCVAKVTRRMPDAFISPEGNYVTQAFLEYARPLVGALPPCA
                     RFSQLRRCGGGPTPGKRKKA"
     gene            5823..6287
                     /locus_tag="PLZ74_03205"
     CDS             5823..6287
                     /locus_tag="PLZ74_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97361.1"
                     /translation="MMAETGSTTETGGTQETLSRTELQIEQEKLKLERERILLERERL
                     EAVRDRMRAEQSLRTDGKGRLAVPLSTLAMLSIICLLVGGILGAFSTSLHVDRRNTAR
                     LQEVMQTLATASPESIGVVTNADHAASAELPAWLKAMKPKGAHAGISLVVIQ"
     gene            6303..7688
                     /locus_tag="PLZ74_03210"
     CDS             6303..7688
                     /locus_tag="PLZ74_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOR97362.1"
                     /translation="MAFFKAYDMRGVFGRDFDLDMVWRIGRWLPSVLGARRMLVGRDA
                     RVSSEPIAEALCRGLTEAGCAVEDIGLATTPMVYFFTAQDGYDASVQITASHNPAHHN
                     GMKVSRAGAVPVGYDTGLAEIKARVASGTLPPPAPVPGRRSDIDVRDRFIAWLRERCP
                     DLTGLKLAVDCSDGMAGLVIREVLGEGPLYLNATPDGRFPNHSPNPLEAESRVQVAEA
                     VRKHGLDVGVIFDGDADRVMFVDETGGFVQPDYLIPVIARTFLQREPGATVLHDVRTS
                     RGVIEALREDGARPVMGRVGHAFAKVRMRETGAVCGGELAGHYYFRDFFCCDSGELAA
                     LMVLGVLAEQRRRGVTFSAHIAPIRRYANSGECNYRVRDPDAAIAAALRAAEAFGTVV
                     GRSEIDGVRLEWTDGWLSLRPSHTEPYLRLIVEAEDGERLARRLDVLETALAPFAEEG
                     SVAVRGADGHA"
     gene            7681..8418
                     /locus_tag="PLZ74_03215"
     CDS             7681..8418
                     /locus_tag="PLZ74_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR97363.1"
                     /translation="MLEIDHISFSYGRRRVIDDLSFDVAPGEIVALTGPTGVGKTTLL
                     QILANLLIQDTGNIRLDGVDPLERPIRYRRGIGYLSEMCPLYDEMTVEAYLTYRVRLK
                     GERTLRIRRRVSEALELCGLDKVGREPVRLLSQGYRKRLSLADALALRPKVLLLDDPL
                     AGLDAQLRRRLGAMLSAISSRSAVVVAGHEIQEMLGWCTRVVLLRTGGSALFYRTGEH
                     SREALTEQVGRQVAADTGEEEACVPST"
     gene            8400..9143
                     /locus_tag="PLZ74_03220"
     CDS             8400..9143
                     /locus_tag="PLZ74_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR97364.1"
                     /translation="MRTVHMIWSREVRAFVRAFSCGVSVAAFLAVAGWTFVMLMRRYE
                     GSVLQVQTLWGLSVAPWLPLLSAVVTMRLFAEERASGMIELLLAAPLRERDLVIGKFL
                     SALTIIAVALSVAVLVPALVLPSLCPAVQGLVRASALAATSFLLLAQASAWCAVGTLI
                     SVLFRNPAAAAVCAMVVCGGLPVASYAVVLAWMPSVRSGVAWMPLLAQVYDFSTGQFS
                     TGVLILYGSITLFCLFACSKALACLRLRG"
     gene            9167..10678
                     /locus_tag="PLZ74_03225"
     CDS             9167..10678
                     /locus_tag="PLZ74_03225"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOR97365.1"
                     /translation="MARVRINQKRVRNAGRRGLRATSMLAVGLGAVLALLVVILGYRL
                     DLTWEVPVGRVRVVSERTRDVLADTQGTIRILCFMDRRHLLFRPVSRLLQGFRRASRS
                     VAGAEIRIEYVDPRWDLTRAGQLAAWGVPENALVFERQRRRIIVTLDEMMAEPSPLQD
                     GTLPDRRRWQGAGPGVFRGERVCAAAVARLALPYERPVIYWLQGHGEGRFDEYDPLHG
                     FSDIARELKRDGFDVRELALPGLDRIPEDGQVLIIAGARRALAAEEVALMDSFLQRGG
                     RLLYLAAPDANTALEPLLEKWGIRVLPLVAVSPRTLSGTEVVVTDFADHGITRNLRNA
                     SVVFGQARCLEMVADPAFTAGADRPQVTPLAQTGPEGWGESRPDVFPRVFDAQSELRG
                     PVTLAAVSERGGTVARDVAFKPTRLCVIGEANFVMNGTLAAQANANRDLFMNAVTWLA
                     GIDVGTAPSLGGDATLHTGFGRRQWVVFLVWSALAVPAAVLALSFLIWARGRW"
     gene            10686..12536
                     /locus_tag="PLZ74_03230"
     CDS             10686..12536
                     /locus_tag="PLZ74_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOR97366.1"
                     /translation="MGNRRVLYFLASCILFCLALLWAIQQRRVRSFSESDKSRARLIE
                     QALDGVERITVEHGDARIGLLRRGAEWELVAPFPARVDQGAVARMLDGFETARVTDAL
                     SFQEVRRRELTLRDFGLAVPRTRVELAGREGVNRVLFGAFTPHGTDVYLLMNGLEQVL
                     AVPSPLYRVLPRTADEVRSRSLVCGNRARIRSLEMRMSGHPFIRLSKEQGTWRLTQPA
                     AAPASDARVEALLDVLYREPVERFVWPTVSNVLDVAGSEADFKLRLELYGLGAESGLQ
                     VQVEEVGGTAAERVVFGRTVEGAAALTYVLLPGGNAVGAVSNAVAQAFRLLPADLRDP
                     RVFAGVRGVVRRLDIYVGDNLFVLSRTNALWRIEAPVSDAADQEVADEAVSRLLRLEA
                     EGVEEAASPEAQAGTAAPSRYAPVSHVVLFGDQGETRFTVTRAEPDAPFVRLAFTNSA
                     ALYRVAASNLPSVFMSQDGLMSLRDKTVLALPETTVRRVTLKRADGSVETAQRDSAAD
                     VWRLADGQTGVLSAERLGRLLKSLTLLRAERIEKIGLTPEEGHVYGLTRPWLEMSVDV
                     DAKDSVRKTLLVGDEADLGSRYAVVRGLDVLFVLGGDAVAELAAHWIEPL"
     gene            12557..14128
                     /locus_tag="PLZ74_03235"
     CDS             12557..14128
                     /locus_tag="PLZ74_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOR97367.1"
                     /translation="MLETVMEFLNGPLGRECFRLNRVLFLVFLALATLAVWRAPAPRP
                     GKVGFGLLRGLTTAALLAILIYQATWQISGFLKPEFVHFLRRYNKRPGAAAMQVMRGP
                     LLDRRGHVLAAPVPGDVWGRRYPLGEAGVHPLGYYHSQFGITAVERVCDPELSGYSVE
                     RREGFPGATLFAVRAEEGRAVTLTLDARLQARAYDLLAGRKGAVIVMRPRSGDLLALV
                     SSPGFNPGDPGPASRDEENAPVFNRATQGRYPPGSVFKILVAAAALDKGLAPVYACPA
                     MGYIAGPNTPPIRDSEYYAYARRGAVWPGWGRLNLEKALVHSSNVYFAQLGVACGAEV
                     FNAMMARAQINAPLVYLQAASGELRSAAGSAPTVTQPRKLAMLAIGQGDLLVTPLHVA
                     CFTAAVAADGVMPRPRLRTDEAPGRMAALCSPEAAGRLTGMLRAAVRNGTGRAAEIPG
                     LEVCGKTGTAQAAGGDDHAWFTCFAPRRKPRLVVTVLVERGGFGARSALPAARALLEE
                     AVRLGLARPEGEGES"
     gene            14125..15405
                     /locus_tag="PLZ74_03240"
     CDS             14125..15405
                     /locus_tag="PLZ74_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOR97368.1"
                     /translation="MSARRTRVVSPVGAVAGLFLLSFLAVLSASLSVLGIKLGNAPVM
                     PWEQLIPLGIYAVGVPAVLVWMRVNGWKGDAGLAGAVFLLCGLGVTVQFRMGSFSRGL
                     ADPMALAPLPLGLLAFAAGVALTAKRRGAWLARTGWAAYLAALGVLAALLLLGRKYRG
                     GLYLAGNINPTELVKPLLVLFLASFLSRRQKAFSETQVGIPVPSLDALLVLIFLWAGP
                     VLLTLAVKDLGLLLLLNAILVVMLFAVARSTGYLALGLAAVTAAGYGVRFLSPHVRAR
                     FDVWLNPFEDPTGKGWQILQALAAMYSGGWWGAGLGSGVPQTVPIVTSDFVYAALAEE
                     IGLAGCALLLAVYAVLFSRGFRCAGAARTPFERLLGVGMTGALAMQTILNVAGVTKAL
                     PMTGITLPLISHGGSSLITTLLMAGLVTGLSDRR"
     gene            complement(15424..15777)
                     /gene="secG"
                     /locus_tag="PLZ74_03245"
     CDS             complement(15424..15777)
                     /gene="secG"
                     /locus_tag="PLZ74_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOR97369.1"
                     /translation="MQILIGFLYVIEVFVCFLLAAVILLQKPKEGGLGVSFGGGMGEA
                     LFGAQMGNVLTQATIILGAIFLINTLALSRLTAHTGSSLMEGVKPSAPAAEQALPFTQ
                     QPAAPTAPAAPAPMP"
     gene            complement(16431..16781)
                     /locus_tag="PLZ74_03250"
     CDS             complement(16431..16781)
                     /locus_tag="PLZ74_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97370.1"
                     /translation="MVDGVTVSVFGYGHGITGRRVWQEDCGLCHSHNAFLYNAPGEVT
                     NAVLRADNYDYSYDAIGNRVWSAVNTLTNSYTANALNQYTLISNPVNPVNPVQIPPPI
                     GACGPPFGNRWLET"
     gene            complement(16878..17090)
                     /locus_tag="PLZ74_03255"
     CDS             complement(16878..17090)
                     /locus_tag="PLZ74_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97371.1"
                     /translation="MKRAAYAMIASAATLTASAATLDAPRLPVPPFADREVSAAHALP
                     AGRADGPRRVPDRRARQHLIYSGQNS"
     gene            17479..17895
                     /locus_tag="PLZ74_03260"
     CDS             17479..17895
                     /locus_tag="PLZ74_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HOR97372.1"
                     /translation="MKGKTQTVSPERRAQLQAELRDAVATRQTGYREKALRILPHVCA
                     SCGRSFEGKRLKELTVHHKDHNHRNNPPDGSNWELLCIYCHDHEHEKTLLGGYYEDAP
                     ARKDVLKPSIFSPFSVLDDLSPVTPDTDAATPDATP"
     gene            18127..>18687
                     /locus_tag="PLZ74_03265"
     CDS             18127..>18687
                     /locus_tag="PLZ74_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97373.1"
                     /translation="MRGAGRRIRGVGWLGLAFMAGSLGAQSIIDGVLQGVPPPPAQPG
                     DETWTFVNDLKQPLWAENHAWPQRAAEAGEADLSRGARVIQGFPDPEGVLETAVDDLR
                     AFLAAGRVLPDGPYVIETGRIETPVFESYRIEVEPNRCRILAADTEGVRRGIVYVEDR
                     MLAAEGPFLPLGSVTRKPFIASRISRC"
BASE COUNT         2963 a         5186 c         6748 g         3790 t
ORIGIN      
        1 gggtttcagg gtttctggtt tcatggtttc atgccacgca tgcggcgtaa attgtggccg
       61 ggggaataag tcttccgtca gaaaccaaca tctgaagtgg cggaggcccg ggcttgtgct
      121 atactgaccg aaatgtccat ggaagtgact tttttgggta ccggaacgtc cgtcggggtg
      181 ccgatgatcg gatgtcgttg cgcggtctgc acttcggcag acccccgcaa cacgcgtcgg
      241 cggactagcc tgtacgtgcg gacgcccgga gccgccctgg tgatcgacac gccgccggat
      301 ttccgtcagc aggctctgga cttcggtgtg gatcgggtgg atgcggtggt gtttacccac
      361 gcccacgcgg atcacatttt cggtttcgat gacatccggc gtttcaatac gctgcagcag
      421 aaggtgattc cggcctatgc ggatacggag acgcttgcgg atgtgcggcg cgtcttcagc
      481 tatatcggcg accgcccgtc gccgttagga ctctaccggc cgctggtcga gttcatcgag
      541 gtccatggac cgttcacggt gggcgatgtg cagttgacgc cgttggatgt gtgccacggc
      601 aacaagatga cggggtatct gctggaaagg gaggggtgcc ggatcgggta tgtgcccgac
      661 tgtcacacga tgcccgatgc gaccgtccgg aggttgcggg gcgtggatgt gatgattctg
      721 gatgcgctgc gctataaacc gcatccgtcg catatctgcg tggaggagag tttacggctc
      781 ctgtccgaga tcgatgcggg ccgctcgtat ctgatccacc tctgccatga tgtggatcat
      841 gcggagttgg aggcgcgtct gccggagggc gtgcaggtga gttttgacgg attgcggatc
      901 acggtggacg agactgcggc ggcgcggccg cttcccgtcc gcgaaaggag tgcgggatga
      961 cggggttctc aagaatacgg aggtgcgcgt gtttggcggg gggcgtaatg tatgcggcgg
     1021 cggtcgcggc gcagcaggtg gaggtcagac tgacgctcgc ctacaacggg tttgtcatcg
     1081 gcgaaccggt gctggtggag gtggaactgc tgaacgccac ccgcaatccg gtcgtggtgg
     1141 gggaaaaggg tgcccagagc gcgttgttca tggacgtgtc tttcggtgac gcctacaatc
     1201 tgctcgcccc ggtgaatccg gaaccgctgg ccggggcatt cacgctcaat cccggtcaga
     1261 cgttccgccg gaagctcgaa ctggacaagt ggtatccgct cgttcgcgag ggacgctaca
     1321 tgacgcagct cgtgctggcg catgagggca tgcggtatga gtccgttaaa aagagcttcg
     1381 atgtggtgcc cggcattcca gtgtgcgagg gggtgcagat gtttgtcaac cggcagaagt
     1441 tgaaacgcca cttcaaactg gtctactggc atcgtaatca gaccgaccgg ttgtttttgc
     1501 ggaccgagga tgagcctggg gcgctaatct gggacgcgat cgatctcggt atcttgctgc
     1561 gcggcactac gcccaaactc gatatctcgc cccagggcga ggtgaccgtt gtccatcgct
     1621 cgacgcagga tgcgtttctc cgcacggtgt tgtggtcgct gcccgacagt cttgagatcg
     1681 tggagcgcaa ctcgctgatg gatccggaaa tttcggcgtc gcagcgggtc aagtcgctgt
     1741 acggggagat ggccgaaggt cagaccgggg aagagaagaa ggcgtggtgg aagttctggt
     1801 gagccgcggg agggcggtgg gggtgcccgg cgcatggtgg ttttagggat cgagacgtcg
     1861 tgcgacgaaa ccgcggccgc agtggttgct gacggtcggc gcgtgctgtc gaacgtggtg
     1921 tacagccaag tgccgctgca tcaaccctat ggcggtgtgg tgccggagat cgcttcgcgc
     1981 agtcatgtcg agaagctgcc cggtgtggtt gccgaggcgg tacgcgcggc cttcgcgccg
     2041 gagtctcctg cgggatccgc gccgccacct gcggcgtggc gggaggtgga tgcggttgcg
     2101 gtgacctacg ggccgggatt ggcgagttcg ctgatcgtgg gtctgtcggc tgccaaaggg
     2161 ctcgccttgc ggctggaacg gccgttgatc gggatgaacc atatcgaggc gcacatccac
     2221 tcggtctttc tgaacccgga ggcacccgat ccgcgcgaag ccgcgccgct gctggcgttg
     2281 gtcgtatccg gcgggcactc atgctggatc gagacggatc ggcccgggaa ctaccggatg
     2341 atcggacaga cactggatga tgcggcgggt gaggcgtttg acaaggcggc caaactgttg
     2401 gggttggggt atcccggcgg tccggagatc gaccgtctgg cgcgtcaggc ggcgcgtgtg
     2461 gacgcggtgg cctttccgaa agggagtccg cggccgggca atcccgcgct ggcgggattg
     2521 cgcgccgaac tgtgcgtgag tttcagcgga ctcaaaacgg cgctcttgta tcatctgcgc
     2581 tcccatccgc cgcaggatca gaacgaagtg gccgaggtgg cggccgccta tcaggaggcg
     2641 atcgtcaccg cacttgccga acggtcggaa cgggcgttgc ggggcgggcg atacgccgcg
     2701 ttcgcggtgg gtggcggcgt gtcgctcaac cggaggttac gcgagcgtct gacagaggtg
     2761 gcccgcgcgg cgggcgtgcg tttgctgttg gctgagccgc gtttctgtgg ggataacgcc
     2821 gcgatgatcg cgggactcgc gggggcgggc ggtggtgtgc gcggacctgc cgcgcaggaa
     2881 ctggatgcgt cgccggcact tgcggccggg gaggagggaa cggcatggtg agggtgacgc
     2941 ggcgcgtttt gttgtccgga gtcgctgtgg cgtggttgct gtgctccggt gtccttgcgg
     3001 cagatcccga tccgctggtg gtggccagtt ggaatgtcga gaacctcttc gacacggagg
     3061 atgacccggc caacaaggga gacgacggct tcacgccgcg gggctggatg cgctggactg
     3121 cggcgcgtta ccggttgaag ctcgagcatc tcgcggagat catcgcaggc atgcggccgg
     3181 atgtgctctg cctgatggaa gtcgagaacc gccgtgtgct cgaggatctg gtgcagacgc
     3241 tggagcgcaa acacgcattc agccttccgg tgatcgtgca tcgggacgca gaggagaaac
     3301 gcggcatcga tgtggcgctg ctggcacgtg ccgcgcctgt tgccacgcag tggctgcgta
     3361 ccgcgccggg acagcgcgag gtggtggcct gcgatttttc ctttggcggg cggcggctga
     3421 cggtgttggt gaaccattgg aaatcgcagt tcggaaaaaa gggggagagt gacgagatcc
     3481 gacagcggga agcgcgtgcg gtacgggcct ttctggacga gcggttgcgc gcggaaccgg
     3541 ccgccgcgat tctggtggcg ggcgacttta atgacgcgcc ggactcgccg atcctgaccc
     3601 aggatgccgg attcgtcatg gatgaggcgc gggtgcgcgg cgacgcatcc ggtcggctgc
     3661 tgttcaatct ggcgggttcg ctttccgagt cggcgcgcgt gacctactat tatgcggcca
     3721 gcaagaagtg gtacacgatg gatgccatca gcgtgacgcg cggcatgctc gccgatgccg
     3781 tgcctgcggc gccctggcgc gtgcggaaaa gcgggtatac ggttttcaaa actgcggcgc
     3841 agcaggactc gcagggcgcg ccgatcccct tccggcgtgt gcgaaacaaa aaggagggcg
     3901 attcctttcg caccggctac tcggatcact tcccggtgcg cgttgaattg gagccgtccg
     3961 cagcgccgta agggtgcggc cggggcgcgg tccgggtgtc acggtatggg cacggagtcg
     4021 accatcaggc gcacctggcg gatttggcgc tcgtccccgg cgaatgcgcg caccgcctgt
     4081 tcgatccgtt gaaggtggtt ccggcggatc tcaaacaggt gggtggaaga ggtcacgaac
     4141 acgaacagga tgcccgcgtc gtatttgccg ggacgggtga aacgcgacac ctgcggcggc
     4201 agaatctcat gccaggccgc agccaggtca tcgagccaag gggtggcgcg gagttcgaga
     4261 cgctgcagga tggtttcaat ggcgtggccg agcggcgtcg gttcctgacg cagttccggc
     4321 gggtcgctgt cgatcggcag gcccggtttg acgcgctgga acagcgtgcg gaccgcggcg
     4381 aggcgggctg cttcgcgtgg cgtgggggtg caccgcgcac ggctggcggc ggagaggaaa
     4441 gcggactcgt ccggaacggc gtagccctcc gactcgctct cccccgggat cggttcccct
     4501 tccgcaaacg gatgtttgcc tttacgcatg cggacagggt atcatatcgg aaccaatcag
     4561 gtggaaagaa aatgacgaca tgaaaaaagg aaacatgctg atcgcccaaa gcggcggtcc
     4621 ctcgatggtc atcaaccaaa gtctggtcgg cgccgtgctg gccgctcggc gcagcggctc
     4681 cggggtcggt aagatctatg gggcgctgca cgggatccgc gggatcctcg acggggcgtt
     4741 catcgatctg cgcaaggaga cgacggctac gcttgaggcg gtcgccgcta cgccttcgtc
     4801 ggctctgggt tcggtgcgtc acaagccttc ggcagaggat tgcctggcgg tgttccgccg
     4861 gttgcggcag cgcgacatcc gttatttttt ctacatcggc ggcaacgact ccgccgaaac
     4921 ggtgcacatt cttcatgagg aggcggtgaa gaatgagtgt gatatccgtt gtttccacat
     4981 tcccaagacg atcgacaacg atttgtgcgg gaatgaccat acgccggggt acggcagcgc
     5041 ggcccgtttc gtggcgtgtg ccgtgaaggg agacgatctc gacaaccgcg cgctgggggg
     5101 ggtcaagatc gatgtgatca tggggcgcaa cgcgggtttt ttgaccgctg ccgcggcgtt
     5161 ggcccgtacg gaagaggatg acggcccgca cctgatctat ctgcccgagc ggccgttcag
     5221 cgtcacgcgt ttcgtgcgtg acatcaaggc ggcgatgcgc gactacggac ggtgtgtggt
     5281 ggcggtctcg gaagggatca cggaccggcg cggcacgccg ctggtggcgg cgttcacgcg
     5341 cgagaaggat tcgcacggca acgtgcagtt gagcggcacc ggtgcgctgg gcgatctgct
     5401 ggccaatgtg gtcaaaacgc gtacgggcat cacgcgcgtg cgggccgaca cgtttggata
     5461 tctccagcgc tcctttccgg gggtggtgtc gccgacggat gcgctggagg cgcggaaggt
     5521 gggtgaggag gcggtccttg cggcggtcgg gaagaatatg gcgagcggat ccatcgcgat
     5581 ccggcgaaaa ccgggcaagc ggtacggggt gcgattcgag gtggtgccgc tgcattgcgt
     5641 ggccaaagtg acgcgccgca tgcccgatgc gtttatcagt ccggaaggca attacgtgac
     5701 gcaggcgttt ctcgaatatg cccggcctct ggtgggcgcg ctgccgcctt gcgcgagatt
     5761 ctctcagttg cggcggtgcg gcggcggtcc gaccccgggc aaacggaaga aggcgtgatg
     5821 atatgatggc agagacgggc tcaacaacag agacgggcgg gacgcaagag acgctctcca
     5881 gaaccgagtt gcagattgaa caggagaaac tgaaactcga gcgggagcgg atcctgttgg
     5941 aacgcgagcg tcttgaggcg gttcgcgacc ggatgcgggc cgagcagagt ttgcgcacgg
     6001 atggcaaggg gcgcctcgca gtgccgctct ccacgctggc gatgctctcg atcatctgtc
     6061 tgttggtcgg cggcattctg ggggcgttct cgacctccct gcatgtggac cggcgcaata
     6121 cagcccggct gcaagaggtg atgcagacgt tggccaccgc ttcgccggaa tcgatcggcg
     6181 tggtgaccaa tgcagatcac gcggcttcag ccgaattgcc cgcatggctt aaggcgatga
     6241 agcccaaagg cgcgcatgcg ggcatttcgc tggtggtgat ccaatagacg ggaaagggca
     6301 ggatggcatt tttcaaggct tatgatatgc ggggcgtgtt cggtcgcgat ttcgatctcg
     6361 acatggtctg gcgcatcggg cgctggctac cgtcggtgct cggtgcgcga cgcatgctcg
     6421 tggggcgcga cgcccgtgtc tcctctgagc cgatcgccga ggcgttgtgc cgcggactca
     6481 cggaagcggg gtgtgcggtg gaggacatcg gactggcaac cacgccgatg gtctattttt
     6541 tcacggcgca agacggatat gacgcttcgg tgcagatcac ggcctcccat aacccggcgc
     6601 accacaacgg catgaaggtg tcgcgtgcgg gtgccgtgcc ggtggggtac gataccggac
     6661 ttgcggagat caaggcgcgc gtggcctcgg gcacgctgcc accgcccgcg ccggttccgg
     6721 gacgccgcag cgatattgac gtgcgcgacc gtttcatcgc ttggctgcgc gaacggtgtc
     6781 ccgacctcac gggactcaag ctggcggtcg actgttccga cgggatggcg ggactcgtga
     6841 tccgcgaggt gctgggagag gggccgctgt atctgaacgc gacgcccgac gggcggttcc
     6901 cgaatcattc gcccaacccg ctggaggcgg aaagccgcgt acaagtggcg gaggctgtgc
     6961 ggaagcacgg gttggatgtc ggcgtgattt tcgacggcga tgccgaccgg gtgatgtttg
     7021 tggacgagac cggcgggttc gtgcaaccgg attatctgat tccggtgatt gcgcgcacct
     7081 ttctccagcg tgagccgggg gccacggtgc ttcatgacgt gcgcacgtca cgcggggtga
     7141 tcgaggccct gcgcgaggac ggcgcgcggc ccgtgatggg ccgtgttggc catgcgttcg
     7201 ccaaagtccg gatgcgcgag accggggctg tctgcggcgg cgaattggcg gggcactact
     7261 atttccgcga ttttttctgt tgcgattccg gcgaactggc ggccttgatg gtgctgggcg
     7321 tgttggcgga gcagcggcgt cggggcgtga ccttcagcgc gcacatcgcg ccgatccggc
     7381 ggtatgcgaa ttcgggcgag tgcaactatc gtgtccgcga tccggatgcc gcgatcgcgg
     7441 cggcgttacg cgcggccgag gcgttcggga cggtggtggg gcgtagcgag atcgacgggg
     7501 tgcggctgga atggacggac ggatggctga gccttcgccc gtcccatacc gagccctatc
     7561 tgcggctgat tgtcgaggcg gaggacggtg aacggctggc gcggcgcttg gacgtgctgg
     7621 aaaccgcgtt ggccccgttt gcggaggaag ggagcgtcgc ggtgcgcggc gcggacggac
     7681 atgcttgaga ttgaccacat ctcgttttct tatggacggc ggcgggtgat cgacgacctt
     7741 tcgttcgatg tcgcgccagg ggagatcgtg gccctcaccg gtcccaccgg cgtgggcaag
     7801 acgacgctgc tccagattct ggcgaacctg ctgatacagg ataccggaaa catacggttg
     7861 gacggggtgg atccgttgga gcgccccatc cggtaccgcc gcgggatcgg ttacctgtcc
     7921 gaaatgtgtc cgctttatga tgaaatgacg gtggaggcct atctgacgta ccgcgtgcgg
     7981 ctcaaaggcg agcgcaccct gcgcatacgc aggcgggtta gcgaggcgct ggagttgtgc
     8041 ggactcgata aggttggccg cgagcctgtc cggctccttt cgcaggggta tcgcaaacgg
     8101 ctgagtctgg cggacgcgct ggcgctccgc ccgaaagtgc tgttgctgga tgatccgctc
     8161 gccggactcg acgcccagtt gcgcaggcga ctcggcgcga tgctgagcgc gatctcatcg
     8221 cgttccgccg tggtggtggc gggacatgag attcaagaga tgctcggctg gtgtacgcgg
     8281 gtcgtgttgc tgcggacggg cggttcggcg ctgttctacc gcacgggtga gcattcgcgt
     8341 gaagcattga cggagcaggt cgggcggcag gtggcggccg acaccgggga ggaggaggca
     8401 tgcgtaccgt ccacatgatc tggtcgcggg aggtccgggc ctttgtccgc gcgttttcgt
     8461 gcggggtgtc ggtggcggcg tttctggctg tcgcgggctg gacctttgtt atgctgatgc
     8521 ggcgttacga gggcagcgtg ttgcaggtgc agacgctctg gggtctctcg gtggcgccgt
     8581 ggctgccgct tctgagcgct gtggtgacga tgcgtctctt tgccgaagag cgcgccagcg
     8641 gcatgatcga gctgttgctg gccgcgccgt tgcgcgaacg cgatctggtg atcggcaaat
     8701 tcctgtcggc cctgacgatc atcgccgtgg cactgtctgt ggccgtgctg gttccggcct
     8761 tggtgttgcc gtcgctctgt ccggcggttc aggggttggt gcgagcgagc gcgctggccg
     8821 caacgtcctt cctgctcttg gcgcaggcat ccgcctggtg tgccgttggt acgctgattt
     8881 cggtgctttt ccgcaatccg gccgcagccg cggtgtgcgc gatggtcgtg tgcggcggcc
     8941 tgccggtggc gagctacgcg gtggttttgg cctggatgcc ttcggtgcgg tccggggtgg
     9001 cttggatgcc gctgctggcg caggtctatg atttttcgac cgggcagttc tccaccggcg
     9061 tgctgatcct ttacggatcg atcacgctat tctgcctgtt cgcgtgttca aaggcgttgg
     9121 catgcctgag gttgcggggt tgattccggg aagacggagg aaaaacgtgg cgagggtgcg
     9181 catcaatcag aaacgggtcc ggaacgccgg acggcgtggg ctgcgggcca cgtcgatgct
     9241 ggcggtcggt ctgggcgcgg ttttggccct gctggtcgtg atcctggggt accgtcttga
     9301 cttgacctgg gaggtgccgg ttgggcgggt gcgtgtggtt tcggagcgta cgcgcgatgt
     9361 gctggccgac acgcagggaa ccatccgcat cctctgtttc atggaccggc ggcatctgtt
     9421 gttccggccg gtgtcgcgtc tgctgcaagg cttccgccgc gcttcgcgca gtgtcgccgg
     9481 agcggaaatc cgcatcgaat atgtggatcc gcgatgggat ctgacgcggg ccggacagtt
     9541 ggcggcctgg ggcgtgccgg agaatgcgct ggtgttcgaa cgccagcggc gccggatcat
     9601 tgtgaccttg gacgagatga tggccgagcc ctcgccattg caggacggaa ctcttcccga
     9661 ccggcggcgt tggcaggggg cggggccggg cgtgttccgc ggagaacgcg tgtgcgcggc
     9721 cgctgtggcg cggcttgcgt tgccgtacga aaggccggtg atctattggc tgcagggcca
     9781 cggcgaggga cgtttcgacg agtacgatcc gctgcacgga ttttcggata tcgcgcgtga
     9841 gttgaagcgt gacggctttg acgtgcgcga actcgcgttg cccggacttg accggatccc
     9901 cgaggacgga caggtgctga tcattgccgg tgcccgccgt gcgctggctg cggaggaggt
     9961 cgcgttgatg gactcctttc tgcagcgggg cggccggctg ctctatctgg cggcaccgga
    10021 cgccaacacg gccttggagc cgttgctcga gaaatggggc atccgggtct tgccgctggt
    10081 ggcggtcagt ccgcggaccc tctcgggcac cgaggtggtc gtcacggatt ttgcggatca
    10141 cgggatcacg cgtaacctgc ggaacgcgtc ggtggttttc ggacaggcac gttgcctcga
    10201 gatggtcgcc gatccggcgt tcacggcagg cgcggaccgt ccgcaggtga cgccgctggc
    10261 gcagaccgga ccggaaggat ggggggagtc gcgtccggat gtgtttccgc gcgtgtttga
    10321 cgcccagagc gaattgcgcg ggccggtcac gctggctgcg gtgtcggagc ggggcggcac
    10381 ggtcgcgcgc gatgtggcgt tcaagcccac gcgtctgtgt gtgatcggcg aggcgaattt
    10441 tgtgatgaac gggacgctgg cggcgcaggc caacgccaac cgtgacctgt tcatgaacgc
    10501 tgtgacctgg ctggcgggta ttgatgtcgg taccgcgcct tcgctcggcg gcgatgcgac
    10561 actccacacc ggattcggtc ggcggcagtg ggtggtgttt ctggtgtggt cggctttggc
    10621 ggtgccggct gcggtgctgg cgctctcgtt tctgatctgg gcgcgcggac ggtggtgagg
    10681 gagttatggg gaatcgtcgg gttttgtatt ttttggcctc ctgcattctg ttctgcctgg
    10741 cattactgtg ggctatccag cagcgccggg tgcgctcttt ttccgagtcg gacaagagcc
    10801 gcgcccgatt gatcgaacag gcgctggacg gggtggaacg gatcacggtg gagcacggcg
    10861 atgcccgcat cggtctgttg cggcggggtg ccgaatggga gttggtcgca ccgtttccgg
    10921 cgcgggtcga tcagggtgcg gtggcgcgga tgctggacgg gtttgaaacg gcccgcgtga
    10981 ccgacgcgct cagttttcag gaggtccggc gccgcgagct tacgttgcgc gatttcggtc
    11041 tcgctgtgcc gcgtacgcgg gttgaactgg ctgggcgcga aggggtcaac cgggttttat
    11101 tcggcgcgtt cacccctcac ggaacggatg tctacctgtt gatgaacgga ctggagcagg
    11161 tgctggccgt gccgtcgccg ctctaccggg tgctgccccg cacagcggat gaggtccgct
    11221 cgcgtagctt ggtgtgcggc aaccgcgcgc ggatccgttc gctcgaaatg cggatgtccg
    11281 gccacccgtt catccggttg tcgaaagagc aggggacctg gcgtctgacg caacctgcgg
    11341 ccgcgccggc ctcggatgcg cgggtcgagg ctctgctgga tgtgctgtac cgcgaacccg
    11401 tggagcggtt cgtctggccc acggtttcga atgtgctgga tgtcgcgggc tcggaggctg
    11461 attttaagct gcgtctcgaa ctgtacggac tcggtgcgga aagcggtttg caggtgcagg
    11521 tggaggaagt cggtggcacg gcggccgaac gtgtggtgtt cggccggacg gtggagggtg
    11581 cggcggcgct gacgtatgtg cttctgccgg gcggtaatgc ggtcggtgcc gtatccaacg
    11641 cggttgcgca ggcattccgg ttgctgcccg cagacctgcg tgacccgcgg gtcttcgcgg
    11701 gcgtgcgtgg agtcgtcagg cggcttgaca tctatgtggg cgataacctg ttcgtgctgt
    11761 cgcggaccaa tgcgttgtgg cggattgagg cgccggtctc ggatgccgcg gatcaggaag
    11821 tggctgacga agccgtgtca cggcttttgc ggcttgaggc cgagggagtc gaagaggcgg
    11881 cgtctcccga ggcgcaggct ggcacggcgg ccccctccag atatgcgccg gtcagtcatg
    11941 tggtgctctt cggcgatcag ggcgagacgc gtttcaccgt cacgcgcgcg gagccggacg
    12001 ccccattcgt gcgcctcgca ttcaccaact cggctgcgct gtatcgcgtg gccgccagca
    12061 atctgccgtc ggttttcatg agtcaggacg ggctgatgtc gttgcgggat aagaccgtgc
    12121 tggccctgcc ggaaacaacg gtgcggcgcg tcacgctcaa acgtgcggac gggagcgttg
    12181 aaaccgcgca gcgcgactct gcggccgatg tctggcgtct ggctgatgga cagacgggcg
    12241 tgttgtccgc ggaacggctc ggacggttgc tcaagagcct gaccttgttg cgtgccgagc
    12301 gtatcgaaaa gatcggcctg acgccggaag aaggccacgt gtatgggctg acgcgtcctt
    12361 ggctggagat gagtgtcgat gtggacgcga aggattcggt ccgcaagacg ctcttggtgg
    12421 gggatgaggc ggatctgggc agccggtatg ccgtggttcg cgggcttgat gtgttgttcg
    12481 tgctgggagg cgatgcggtg gcggagcttg cggcgcattg gatcgagccc ctgtgacgcg
    12541 tggggcaggg ggtggaatgc tcgaaaccgt gatggagttt ctgaacggac cgctgggccg
    12601 ggaatgcttt cgcctgaacc gcgtgctgtt tctcgtgttc cttgcccttg ccacgctcgc
    12661 cgtgtggcgc gcgcctgcgc cgcgtcccgg caaagtgggg ttcggtctgt tgcggggatt
    12721 gactacggcc gcattgctcg cgattctgat ctatcaggcg acttggcaga tctcgggctt
    12781 tctgaaaccg gagttcgttc atttcctgcg gcggtacaac aagcggcccg gggcggccgc
    12841 gatgcaggtg atgcgcggtc cgttgctcga ccgccgcggc catgttctgg cggcgccggt
    12901 tcccggtgat gtgtggggcc gtcggtatcc cttgggggag gcgggggttc atccgctggg
    12961 ttattaccat tctcaattcg gaatcacggc ggtcgagcgc gtgtgcgatc cggaattgag
    13021 tggctactcc gttgagcgcc gggagggttt tcccggtgcg acgttgtttg cggtgcgggc
    13081 cgaagaaggc cgtgcggtga cgctcacgct ggacgcgcgg ctgcaagcgc gcgcctatga
    13141 cctgttggcg ggacgcaaag gcgcggtgat cgtgatgcgc ccgcgttccg gcgatttgct
    13201 ggcgctggtc agctcaccgg gattcaatcc cggcgatccc ggtcccgcct cgcgggatga
    13261 ggagaacgca ccggtcttca atcgcgcgac acagggccgt tacccgcctg gttcggtctt
    13321 caagatcctg gtggcggctg cggcgttgga caaagggttg gcaccggtct acgcttgtcc
    13381 ggccatgggg tatatcgccg gccccaatac gccgccgatc cgcgacagcg aatattacgc
    13441 ctacgcccgc aggggcgcgg tctggccggg atggggacgg ctcaatctgg agaaggcgct
    13501 ggttcactct tcgaatgtct actttgccca gttgggcgtg gcgtgcggtg ccgaagtctt
    13561 taacgcgatg atggcgcgtg cccaaatcaa cgcgccgctt gtctatcttc aagccgcctc
    13621 cggcgaactc cggagcgccg cgggttcggc accgacggtg acgcagccac gcaaactcgc
    13681 gatgctcgcg atcgggcagg gcgatctgtt ggtcaccccg ctgcatgtgg cctgtttcac
    13741 tgcggccgtg gcagcggacg gtgtgatgcc gcggccgcgg ttgcgtaccg atgaggcgcc
    13801 gggacggatg gccgcgctct gctcgcctga agcggcgggg cgtcttacgg ggatgctgcg
    13861 tgcggcggtg cgtaacggca ccggacgggc tgcggagatc ccgggactcg aagtgtgcgg
    13921 caaaaccggc accgcgcagg ccgctggcgg agatgatcac gcttggttca cttgtttcgc
    13981 cccgcgccgc aagccgcgtc tagttgtcac ggtgctggtc gagcgtggcg gtttcggtgc
    14041 ccggagcgca ctgccggccg cacgcgcgtt acttgaggaa gcggttcgcc tgggccttgc
    14101 tcgacccgaa ggggaggggg agtcatgagc gcgcgccgta ctcgtgtggt gtcgccggtc
    14161 ggagcggtcg caggcctgtt cctgctttcg tttctggcgg tactcagtgc cagcctctcc
    14221 gtgctcggta tcaaattggg caacgcgccc gtgatgccgt gggaacaact gatcccgctg
    14281 ggaatctacg cggtcggcgt gcccgccgta ctggtgtgga tgcgcgtcaa cgggtggaaa
    14341 ggcgacgcag gtttggcggg ggcggtgttt ctgctctgcg ggctcggggt gaccgttcaa
    14401 tttcggatgg gcagtttctc tcgcgggctg gcagatccga tggcgctcgc gccgctcccg
    14461 ttgggtctgc tggcctttgc ggcgggcgtc gcgctgaccg ccaagcggcg cggcgcgtgg
    14521 ctggcgcgga ccggttgggc ggcatatctc gcggcgctcg gtgtgttggc cgccttactg
    14581 ctgctgggcc gaaaatatcg cggcggactc tatctggcag gcaacatcaa ccctacggaa
    14641 ctcgtcaaac cgcttctggt tctgtttctt gcctccttcc tgagccgcag acagaaagcg
    14701 ttttcggaaa cgcaagtcgg cattccggtt ccttctctgg acgccttgct ggtcctgatc
    14761 ttcctctggg cgggaccggt gctgctcact ctggcggtca aggatctcgg gctgttgctc
    14821 ctgctcaacg cgattctggt cgtgatgctg tttgctgtgg cgcgcagcac cggatatctt
    14881 gccctcggac tggcggcggt aactgccgca gggtatggcg tgaggttcct ttcgccgcac
    14941 gtccgcgcgc gttttgacgt ctggctaaat ccgtttgaag atccgaccgg caaggggtgg
    15001 cagattctgc aagcgcttgc ggcgatgtac tcgggtggct ggtggggtgc gggtttgggt
    15061 tcgggcgtgc cgcagaccgt gccgatcgtg acttccgatt ttgtgtatgc ggcgctggcg
    15121 gaggagatcg gactggcggg gtgcgcgctg ctgctggcgg tttatgccgt gctcttttcc
    15181 cgcgggttcc gttgcgcggg cgcggcgcgt acgccgtttg aacggctgct gggtgtcggc
    15241 atgaccggtg cgttggcgat gcagacgatc ttgaacgttg cgggcgtgac caaggcgttg
    15301 cccatgacgg gaatcacgct gccgctgatc agccacggcg gcagcagttt gatcaccacg
    15361 cttctgatgg cggggctggt gacgggcctg tccgaccgcc gatgatgccg cgtcgtgttc
    15421 gcgttacggc atgggcgcgg gcgcggccgg ggctgtaggg gccgccggtt gctgggtgaa
    15481 gggcaacgcc tgctcggctg cgggtgccga gggtttcacc ccctccatga gggatgagcc
    15541 ggtgtgcgcg gtgaggcgcg agagggccag tgtgttgatc aggaagatcg cgccgagaat
    15601 aatggtcgct tgagtcagca cattccccat ctgcgcgccg aacagggcct cacccatacc
    15661 gccgccgaaa ctgacgccca acccgccctc tttcggcttc tgcagcagga tgacggccgc
    15721 cagcaggaaa cagacaaaca cttcgatcac atacaaaaag ccaatcagaa tctgcatgat
    15781 gcgcactccc cttcgttttc cggtagcctt tgccgtacgg aaaatgtctt tttataacaa
    15841 atagtgtgca tctcccgcaa tggcaaaaaa cgcgtcccga gcttttcggg tcaacaaaat
    15901 gtgggcttga tcagatccgt ccggatgact aacataacgg taccaactgg ggtctgcgca
    15961 tctttgtttg gaatcagatg atgagattca gaatggttac agacttggcc tcatggacga
    16021 ccgagcgctt gatgttgagc cttcggcgtc aatttgcata ttcggtcggc gtgttctttc
    16081 tcatgctccc attcttggta cggggcgaga ttcgtggcat catcacgttc tcggttctgg
    16141 aaatctgcgt cgggttcaca ttcctttggc actggcggga actacggcga agacagagcc
    16201 gaatggagac ggataccaag tagcaatcgg ttgcaaaacc gcttccatag cggtaccttt
    16261 cttttcgtgg gtcacggctt gtcattcctt tctttgtttt tgtggcacaa aaagaagaag
    16321 gatagccgtg gcccgccttt ttcaagccag cggttcccga agggggggcc acaggcccct
    16381 ataggtgggg ggaatttgga caggattaac aggatttaca agattggaga ttaggtttca
    16441 agccagcggt tcccgaaggg ggggccacag gcccctatag gtggggggat ttggacagga
    16501 ttaacaggat ttacaggatt ggagatcagg gtatactggt tgagggcgtt ggctgtataa
    16561 ctattggtca gggtgtttac cgccgaccag acgcggttgc cgatggcatc gtagctgtaa
    16621 tcgtaattgt cagccctcag aaccgcgttc gtaacctcgc caggcgcgtt gtagaggaat
    16681 gcgttgtgac tgtggcataa cccgcagtct tcctgccaaa cgcgtctgcc cgtaatgcca
    16741 tggccgtaac cgaacaccga aacagtcacg ccgtccacca tgttgctgac ccccgtgaca
    16801 agatcggggc ggaaaccgtc tcgccaagcc tttcaccccc gacagtcaca cgcgtattct
    16861 caaccctgtt gcgcccgtca agagttctgt ccagagtaaa taaggtgttg ccgagcgcgt
    16921 cggtccggga cacggcgcgg cccgtccgcc cggcctgcgg gcagggcgtg cgccgccgac
    16981 acctcgcggt cggcgaaagg cggcaccggc aggcgcggcg cgtccagcgt ggcggcggat
    17041 gccgtcagcg ttgccgccga ggcgatcatt gcgtatgcgg cgcgtttcat ttttcgtttc
    17101 cttccctggt tttccggcag ggaaaaggcg cggaccccgt tccgtgcctt atctgaggtg
    17161 ccaccaagca cccgccagga agcccggcaa cgaggccgcg cccgatcggg cgcgtccccg
    17221 tgcatcccgc tcctcccaaa gatttgacgg cttttcgtag ggaacgtttt gcgcgaaaac
    17281 gcaaaaatca gtttttaggt tccggtctct tccgcatgcg ttgtccttgc cgattcaatc
    17341 aatccagcca accatcagaa tgaacatgct cctttttgcg gggactttgc acccgttttt
    17401 tagggtaagg gtttctgccg tcgacagtgc gattgaaacg cattgacaag aacggcacgg
    17461 gacgcctatc gtggaggcat gaaaggaaaa acacaaaccg tttcaccgga acgccgtgct
    17521 cagctgcagg ccgaactccg cgatgccgtt gcgacgcgcc agacgggtta ccgcgaaaag
    17581 gcgttgcgta tcctgcctca cgtttgcgcg agttgcggac gtagttttga aggaaagcgg
    17641 ctcaaggaac tgacggttca tcacaaggat cacaaccatc gcaacaatcc gccggacggc
    17701 agcaactggg agttgctttg catctattgt cacgaccatg agcatgagaa gaccctgctc
    17761 ggcggttact acgaagacgc gcccgcccgg aaggatgttc tcaaaccctc gatcttctcg
    17821 cctttttccg tgctcgacga cctgtcgccc gtcacgccgg acacggatgc cgccacgccg
    17881 gatgcgacgc cctgaacccc gtgcgcgcat gtcgggccgt aacagaaaaa agggttgttt
    17941 ttgacgggtt cgaggcttgt tggattcggg ttcctgtgcg aatatgactg cggtggattg
    18001 tgcggtccga gttggatgtg accaaacggt tgccgccaga ttctggagat gctcggggag
    18061 cgcgctcgaa aggtggaacg ggaggacgag atgggatacg ccgcaaggtg gaaagggggg
    18121 gacgggatgc gtggtgcggg aagacggata cggggcgtcg gatggttagg gctcgcgttt
    18181 atggcgggca gtctgggggc gcagtccata atcgatggtg tgctgcaggg tgttcccccg
    18241 cctcccgcgc agcccggcga tgagacctgg acttttgtca acgatctgaa gcagccgtta
    18301 tgggcagaga accacgcttg gccgcagcgc gcggcggagg cgggggaggc ggatctgtcg
    18361 cgcggggcgc gcgtcataca gggattcccg gatcctgaag gggtgctcga aacagcggtt
    18421 gacgatctgc gcgcgtttct ggctgcgggc cgtgttctgc cggacgggcc ttacgtgatc
    18481 gagaccggcc ggatcgagac accggtgttt gagtcttatc ggatcgaggt ggaaccgaat
    18541 cgttgcagga ttcttgccgc cgacaccgag ggggttcggc gcggaatcgt ttatgtggag
    18601 gatcgtatgc ttgcggccga agggccgttt ctgccgctgg gttcggtcac gcgcaaaccg
    18661 ttcatcgctt cccgtatctc ccgttgc
//
LOCUS       DAOSRB010000034        18491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3154_length_18491_cov_6.991864, whole genome shotgun sequence.
ACCESSION   DAOSRB010000034 DAOSRB010000000
VERSION     DAOSRB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18491
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3154_length_18491_cov_6.991864"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLZ74_03270"
     CDS             complement(<1..202)
                     /locus_tag="PLZ74_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97374.1"
                     /translation="MLIMPHPYGRMANRLILASTFIAQAEEYGDSFLHLAFADYCRFF
                     EGTRHRPFLYHRSTHPQPRVTKR"
     gene            complement(196..924)
                     /locus_tag="PLZ74_03275"
     CDS             complement(196..924)
                     /locus_tag="PLZ74_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR97375.1"
                     /translation="METQPELTIVIPVYNEGENILRSLREIQRHVTVPATVSIVYDFE
                     EDTTLAALKQLPAGSSLKVRAMRNAYGRGVLNAIKTGLEAADTELIIVTMADLSDPPE
                     VMNEMVRVARETGAAIVCASRYMRGGQQIGGPKLKGFLSRTAGMILYWVARLPTHDPT
                     NSFKLYRKSFLSTTTIESPGGFELGIELVVKAWKSGHLIAEVPTTWRDRVAGKSNFKL
                     WKWLPHYLRWFFYAFRRNKKHPPC"
     gene            complement(945..1967)
                     /locus_tag="PLZ74_03280"
     CDS             complement(945..1967)
                     /locus_tag="PLZ74_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR97376.1"
                     /translation="MKILVTGSAGFICGYLVEELLNNGHEVVGIDNYSKYGKVEKSYD
                     NHPRYHFTEGDVKDLALMKNLIAGCDQVLGAAAMIGGISYFHELAYDLLAENERITAA
                     TFDAAIWAFKHSSLKKINILSSSMVFESATTFPSREEDVRKCPPPLSTYGFQKLACEY
                     FAQGAFEQYKLPYTIIRPFNCVGIGEKRALCDSEIVSGNIKLAMSHVVPDLVQKVVKG
                     QDPLHILGSGDQIRHYTYGGDLAHGIRLCIEKPEAINEDFNLSTPVSTSVLELAELIW
                     NKIHKGAKPFRYTSDTPFKYDVQKRVPSVEKAERLLGYKAETTLDQILDEVVPWIVEQ
                     VRIGGI"
     gene            complement(1994..3169)
                     /locus_tag="PLZ74_03285"
     CDS             complement(1994..3169)
                     /locus_tag="PLZ74_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOR97377.1"
                     /translation="MTTRICIIGGCGHVGIPLGLAFASRGFPVTLIDCNQTAVEQVSR
                     GSLPFKEEGAEELLHAHINKNLFATTDVSLAAQQDVIVFVTGTPVDEHLNPRVNDVLK
                     VVTSYLRFMNKSQLVILRSTIFPGVTQIVDHLLVQALGSSKLAFCPERIVQGKGIEEI
                     FNLPQIVSATSAQAEQEASELFSAIAKKIVPLSTAEAELAKLMTNAWRYLEFAIANQF
                     YMIVEKKGFDFYKIFNAMKDDYPRAKNFASAGLAAGPCLFKDTMQLSAFHSNEFFLGH
                     SAMLVNEGLPNFLVQQLEEKMGNLKGRKIGILGLAFKADNDDTRESLSFKVKKLLELQ
                     MAEVLLSDIYVAGTLPLQQFMQEAEGIILGVPHTEYRKLAPTCPYIDCWGVWPRPNS"
     gene            3331..4167
                     /locus_tag="PLZ74_03290"
     CDS             3331..4167
                     /locus_tag="PLZ74_03290"
                     /inference="COORDINATES: protein motif:HMM:NF039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HOR97378.1"
                     /translation="MQTWQRTRLAAGQDERYVPSACTEVTALAYHFWDSERFDEQFAF
                     LECAIRETWRWCGLLKTVLVVNRVTPRIEAFAEAFSGWVRFDVLETLIPGNLHSMSAD
                     CNAHLHRRFDTPYVLVVQNDGFPIRSGLDAFVGVWDFIGAPYVRNTWCNRIVCGMLNC
                     WVSNGGFSLRSKRICELAAQYWRKKYCALPASRMVSEDVFFTETLPLRERAYRRAVRI
                     AAYPEAVRFSYDAAFPYTEPVPPFGFHGLKAFEFLRQTGWISDAGDTSAGPEYAPNRP
                     AR"
     gene            complement(4092..5102)
                     /locus_tag="PLZ74_03295"
     CDS             complement(4092..5102)
                     /locus_tag="PLZ74_03295"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR97379.1"
                     /translation="MRPDPNKAMEVTGTRRLTTDERTGLCVVPPMRPIHAILITYHPS
                     DRVLDTVRDAAEQADHVIVVDNSATESARNLLTQAFAPHTDRFTLIFNPVNLGVAAAL
                     NIGMRTAQVGKAHWVLTLDQDSRLTSGMVAALIAAWEAMPREQRSQIGVLAPQTVSAD
                     KTPSENTSAPIANWREVETAITSGCMICLEAWQAVGGFNEALFIDYVDHEFCFRLRWN
                     GWRVVQCPDAWLIHRIGNAEHRRLFGREITVNQHPPVRSYYIMRNGLYFWRAFPEPSA
                     FIRADKRNTLTLLLKALTVEPQRRERLSLFWRGYRDYRAGRFGAYSGPADVSPASDIH
                     PV"
     gene            5233..6081
                     /locus_tag="PLZ74_03300"
     CDS             5233..6081
                     /locus_tag="PLZ74_03300"
                     /inference="COORDINATES: protein motif:HMM:NF039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HOR97380.1"
                     /translation="MVGEETANLFEWQRNRLEVQRSAVFGAPQSSQVTVVTYHFWDVE
                     QADLRFDAVECALYETWRHCGKLKACVVVNRETPRLRAFAEAQGDAVTIRVNPALIPG
                     DLPSMSVDCIVNLHRYFDTDSVLVIQNDGFPLRGGIKAFEGRYDYIGAPFVRDTALNR
                     IMGLWPRFAVGNGGFSLRTKRICEMASFYWSKRWYHHMPRDWRFVREDAFYGVLLPWL
                     ETSYRRTIRFAPVDVALRFSYDDLYGACPAELPFGFHGSAAFREFVEKGLLKQGEQGA
                     AAVSPF"
     gene            complement(6068..6892)
                     /locus_tag="PLZ74_03305"
     CDS             complement(6068..6892)
                     /locus_tag="PLZ74_03305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR97381.1"
                     /translation="MGTFLLSVIIPTRNESRNLTACVESFSDAVAAGWCEVLVVDNNS
                     DDGTPQLAATLGARVFSQGPERSTQRNRGWREAVAPFVLFMDADMSLPPETLAEIHTC
                     LTSPQPPDALYIRERRTGGGWWTRVRNFERGFYNATCIDGLRVFRRALLETTGGYDET
                     LFACEDWDLDRRILALTENIALTNGFLLHHEERLTFRRHLRKKRYYSGSFSAYIKKWG
                     WDPVTRRQFGFWYRFVTVFLENGKWRQALRHPFLLLAVWAERVCVGLLCLRAVKKG"
     gene            complement(6996..7622)
                     /locus_tag="PLZ74_03310"
     CDS             complement(6996..7622)
                     /locus_tag="PLZ74_03310"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOR97382.1"
                     /translation="MMHPPPSSGNPLPPVLRATPDCLSEAARLLCAGGIAVIPTDTVY
                     GIAAHPDRPEAVARLSAIKRRAEDKPIALLAADVGAVRAFGAHFPEGARRLADACWPG
                     ALTLVLACGERWEGFRVPDHAFTRALLAACGGTLRVTSANLSGAMPATSAAQALQDVG
                     LVADRVLDDGLSPGGTPSTVVKAEADGTLTVLREGAIPATAIRRLSRA"
     gene            7777..8820
                     /locus_tag="PLZ74_03315"
     CDS             7777..8820
                     /locus_tag="PLZ74_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOR97383.1"
                     /translation="MEEKVLVTGGSGFLGINLIRYLKGKGVTGIRSIDLEPFDYPEKA
                     EVDAVVGDIRDVEALRKVMEGVTWVVHTAAALPLYTEQDIMTTDVDGTRNVMTLAREA
                     GVRRAVMISSTAVYGIPDHHPLVETDRLIGVGPYGRAKILAEEVCLEARQQGLCVPII
                     RPKSFVGPERLGVFALLYDWAYTGHGFPMIGSGKNRYQLLDVEDLCEAIWLTLTRDEE
                     VVNDTFNIGAKIFGTMREDYQAVLDEAGYGKRIRGFPAAPMIWALRFLECLRLSPLYK
                     WVYETACEDSFVSIEKAERVLGYAPKFSNRDALVRNYRWYVANRETFKNASGVSHRVP
                     WKQGILRFAKIFF"
     gene            9016..11217
                     /locus_tag="PLZ74_03320"
     CDS             9016..11217
                     /locus_tag="PLZ74_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97384.1"
                     /translation="MKRMFCALVTGVLCAAGGVNAANTFTLWTGADGTDPTDLARPAN
                     WEGGAVPVIDTGTMDGPFGKFYSLGTVTVPLTLSEDLFLNGLVFDSVGTAFDLDLTGR
                     TLRLYGYSQDSHSLTFRTFRNVPETIRVRGGTLDMTDGKQKTVYVDGASDVSFSGTEL
                     RGGIQFRTLYGANVCLTNGVTGSAYFTTFAGTKTVIAGTGTEIIGAGTCSIGNTAGET
                     GDVFHVLDGAYWTNANACVIGYAGYGNYGLISNATVYVTGGNLGAGSNGQYASNNVFE
                     VKGGARVHVRGSNGKSLINHGRWGYNNTLRIAGEGTFVDWWTNDHNDGSVVVGEMAMN
                     NLLHVTDGARLGTNGCLGVGFVGNIYYGNWRGDSIGNTLKVDGGAQCYMANLCVGGLC
                     TGRMDPYDGTNGRNTYKRGPNGEWWQNTYFDLSISSNAFIVSGSATRAQLAGSLQVAG
                     KTNEGPCLAYVHENDFNRVRVEDGAELTVGSVSLGKWGVSNHIEVVGGALLSVTTGNT
                     GYYDGFSFGGFPTTGTWVRVSGANSRLSIPNSRLALMGCPDGRENKIEISDGALVTAK
                     HVNSYATNNVFHLNNGTLTVTDSLICPNGDANGAKPRFIFEGDAPRIRCTSTDDGCQF
                     KTEPIFEFRIPEKGFADVPVKSSGHLRIYGEPTLVLDGRCLRSYSAAGGGIQVLFEAA
                     EGKTLTITSERIAKMSGEAAAVLDGCAVTLVDKRKLVLSVPDRSGTLIRLH"
     gene            complement(11254..12420)
                     /locus_tag="PLZ74_03325"
     CDS             complement(11254..12420)
                     /locus_tag="PLZ74_03325"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOR97385.1"
                     /translation="MKKLNDAILAIKPSQTLAISSLAKQMAEQGCHVCNFSAGEPDFD
                     TPDLIKEAAITALRQGKTKYTPVAGVPQLCKALSAKFARDNGLDYKPSQIVVSCGGKH
                     SLALVFQTLLNPGDEVLIPTPFWLSYPEMVRVAGGIPVFIHTTADQSFKITPQHIERL
                     IGPATKALVINSPSNPTGVMYTPEELRALGETALLHDITIISDEIYEKMVYDGQRQVS
                     MASFSKALYDNTITVNGFSKPYSMTGWRLGYTAGPEPFMKAMSALQSHCASAPNTFAQ
                     WGAITALEQGAASVAEMVAAFDARRRRICERMRAIPGVKCVIPTGAFYVFPDISSFGL
                     DSLTFSTRLLETEHMAVVPGAAFGADTCVRLSYACGMDEIEEGMTRFARFCASL"
     gene            complement(12417..13586)
                     /locus_tag="PLZ74_03330"
     CDS             complement(12417..13586)
                     /locus_tag="PLZ74_03330"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOR97386.1"
                     /translation="MAKELAFVLINPYSIRKSRTGGIIARYLVRTDLKLIGARVIGAS
                     AELVNAFATYLETYDPDDPEKCALLANYVRRFHMPDAATGRPHRSILLLFEGDDAIRK
                     IFDVTGSIRQQWCSGQSVRDTYGDLVRNPATGEVEYFEPAVLVGPSQKVVGDILRMWL
                     PHLASQAGIVRGALDVPGDADAQETLVMLKPDNWRQPSLRAGNIMDLLSNSGLRIVGV
                     KKFSMSVAQAEEFYGPVREGLKKVFKRFAADRAAQALSREFGFPVDAAPLDGLCDMLA
                     PSFAEREFESIVEFMTGRRPSNCLPEERTLPGTEECLAIVYEGIDAVSKIRDILGATD
                     PLKARPGSIRREFGTNIMVNAAHASDSVENAHREMRIIDVARDPHFEELIQNYYP"
     gene            complement(13589..15319)
                     /locus_tag="PLZ74_03335"
     CDS             complement(13589..15319)
                     /locus_tag="PLZ74_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOR97387.1"
                     /translation="MNGALLLSLCGVLFLLAYTMYGRLIERILGVDPERPTPAHTLRD
                     GIDYVPTHPAVLFGHHFASIAGAGPIVGPVLAAQFGWVAVALWVVLGCIFIGAVHDLA
                     AIFLSIRHQGRSIGTVIESLMGYWGRMLFLFFCWFTLVLVVAEFLRIVAETFVEIPAV
                     GTASMLFIALAVGFGRLVYRGGMSVLTGSLIFVPLTFACVYFGTLIPLDLVKLLGCDA
                     AVARQIWVVVLLVYCFAASVLPVWLLLQPRDYLNSYLLYAMMALGFVGILVARPALNL
                     DAFTGWHAVSPLSGRSDPLIPLLFVTVACGACSGFHALVASGTTAKQIDNERHLRPVA
                     YGGMLIEGVLAIIALVAVGVMTQGEYTVALKEQGAVKLFAGGVASFTDKLGLPHEAGI
                     IFISLALAAFLMTTLDTATRLARFTWQELFLPRGQSGDVPPATVAPTLPASHAFFSNR
                     YIATLIAVVAAGWLLLGGGAKSIWPVFASSNQLLAALTLLGCSLWLLRHKRPLALTLL
                     PMLFMMATSGAAIVTLFLRNAQAWQADGFKAGGVMTLTTGILIVMSVALIVMGAVSIR
                     QTMRQSERKV"
     gene            complement(15316..15837)
                     /gene="ybeY"
                     /locus_tag="PLZ74_03340"
     CDS             complement(15316..15837)
                     /gene="ybeY"
                     /locus_tag="PLZ74_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOR97388.1"
                     /translation="MSSPNIYIRIGTRRFRLDTAALKRLAAALAKRAQTRLRNGPVWR
                     EITIHLLDDAGITPVNAAILKHAGPTDVITQRYEPIPGEPDGLIGELFVNVERAARAA
                     PRRAGWNADRELALYLAHGFDHLAGAEDRTPAERARMRRRELAWLRHLSLTPLFRSGT
                     IPIQNRSTREFSI"
     gene            complement(15885..17324)
                     /locus_tag="PLZ74_03345"
     CDS             complement(15885..17324)
                     /locus_tag="PLZ74_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97389.1"
                     /translation="MMSRTKTALPHRKAATRRGFLKRTLAASTLTAFPAIRATGRPVT
                     GDKVRLACCGIGNQGASDINNLYATGLCDIVALCDTDMGAPHTRKILDTFPDAPRFQD
                     FRKMLDKLGGQIDALCIGVPDHTHFPIAMMAMGLGKHVYVEKPMAHTFREIELMMKAE
                     KKNRVAAQMGNQGHSGANYFQFKAWTEAGIIRNVTRIVAHMNGARRWHKWNGVVTSLR
                     PAQPVPETLDWDTWLGQVPFHGYHRDYVHGDWRCWYSFGNGALGDWGAHILDTAHQFL
                     ELGMPTEVSALNLEGHNPYVFPMASTLSFKFAARGAMPPVEVLWYDGVKNFPELPPGF
                     GGSAKTADDIPTPGGGSTAVSKLSPGKEIYAEDLTFKGGSHSSTLEIIPAEKARDMAN
                     RLPEYPKNQSNHYKNFLLACMGRETCRSSFEVAGPLSQVLALGCIAQRLNTTFTLDPV
                     TRRITGNKLANDLLDGPPPRKGWEQYYRV"
     gene            complement(17447..18136)
                     /locus_tag="PLZ74_03350"
     CDS             complement(17447..18136)
                     /locus_tag="PLZ74_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97390.1"
                     /translation="MKKLLYIVYVFVAVLTLTVALSMLNAKHEKMNTVATVGGAAISE
                     TNDALSIIVKTLLEQFDKDENIAVSPQEIEKQREFFVKLAKMIYDQRITYLNKELEML
                     NVSGDGTVDVSNKTEKIRKEMAWLEPSFFVPADDVLRTNVLLWKRNKALYKRYGGNVY
                     FSHVGPFPADAYKAFFKEKEAERAFEIFDEKLRSDFWGEQFNYTKQSDLLLDDGKKAI
                     ETPPWEATIDG"
BASE COUNT         3605 a         5229 c         5862 g         3795 t
ORIGIN      
        1 ggcgtttcgt gacgcggggc tgggggtgtg tcgaacggtg gtagaggaag gggcggtgcc
       61 gcgtgccttc aaagaaacgg cagtagtcgg cgaacgccag atgcagaaat gaatccccgt
      121 actcctcggc ctgcgcgatg aatgtggagg ccaggatcag gcggttggcc atgcggccgt
      181 agggatgggg catgatcagc atggcggatg ttttttgttt ctccggaatg cgtaaaaaaa
      241 ccaccgcagg tagtgaggca gccacttcca gagcttgaag ttggatttgc ctgccacccg
      301 gtcacgccaa gtggtcggga cctccgcgat gaggtgaccg ctcttccacg ctttcacgac
      361 caattcgatg cccagttcga atccgcccgg gctttcgatg gtggtggtgg aaagaaacga
      421 cttgcggtac agcttgaagc tgttggtcgg gtcgtgcgtc ggaagccgtg cgacccagta
      481 gaggatcatc cctgcggttc gggacagaaa gcctttgagc ttggggccgc cgatctgttg
      541 tccgccgcgc atgtagcgcg aggcacagac gatggcggcg ccagtttccc gcgccacacg
      601 aaccatctcg ttcatcactt cgggcgggtc agaaaggtcg gccatggtga cgatgatcag
      661 ttcggtgtcc gcagcttcaa gtccggtctt gatggcgttg agaacgccgc gtccgtaggc
      721 gtttcgcatg gcccgaacct ttaaggacga tcctgctggc aactgcttga gcgccgccag
      781 tgtggtgtct tcctcgaagt cgtaaacgat actgaccgtc gctggcacgg tgacatgccg
      841 ttgaatctct cgcagcgacc gcagaatgtt ctccccctcg ttgtagacgg ggatgacgat
      901 tgtcaactcg ggctgcgttt ccataaaaac ttgcgatgtg ctcctcagat accaccgatg
      961 cggacttgct cgacgatcca ggggacgacc tcgtcaagga tctggtccag cgtagtttca
     1021 gccttgtaac ctaataggcg ctccgccttc tcaacggatg gcactcgctt ctgcacatcg
     1081 tacttgaacg gcgtgtccga ggtgtagcgg aacggctttg cacccttgtg gatcttgttc
     1141 cagatcagtt ccgcaagttc gagcacggag gtggagacgg gagtggagag gttgaagtct
     1201 tcgttgatcg cctccggttt ttcgatgcag aggcggatgc cgtgggcgag gtcaccgccg
     1261 taggtgtagt gacggatttg atcgccagat ccgagaatgt gcaacggatc ttggcctttc
     1321 accactttct ggacgagatc aggcacgacg tggctcatgg ccagtttgat gttgccagag
     1381 acaatctcgc tgtcacacag tgcgcgtttc tcgccgatgc cgacgcaatt gaaggggcgg
     1441 atgatggtgt aaggcagctt gtactgctca aaggcacctt gcgcgaaata ctcgcaggcc
     1501 agtttctgga acccgtaagt gcttaagggg ggcgggcact tacgcacgtc ctcctcgcgg
     1561 ctcgggaaag tcgtagccga ctcgaacacc atgcttgaac tgagaatatt gatcttcttg
     1621 agcgagctgt gcttgaaggc ccaaattgcg gcgtcgaacg tggcagccgt gatgcgctcg
     1681 ttctcggcga ggaggtcata ggccagctcg tggaaatagg agatgccgcc gatcattgcc
     1741 gctgctccca aaacttgatc acagcccgcg atcaggtttt tcatcagcgc caagtcttta
     1801 acatcccctt ccgtaaaatg ataacgggga tgattgtcat aggacttctc aactttgcca
     1861 tattttgagt aattgtcgat cccgacaacc tcatgcccgt tgttgagcaa ttcttcaaca
     1921 aggtacccgc aaataaaacc cgctgagcca gtaacaagaa ttttcataac gtgtctcgtc
     1981 cttaggaagt ggtttaactg tttggtcttg gccacacccc ccaacagtct atataggggc
     2041 atgtgggggc aagcttccga tattccgtgt ggggaacgcc aaggatgatt ccttcggctt
     2101 cctgcataaa ttgttgaagt ggcaatgttc cggcgacgta gatatccgac aggagaacct
     2161 ccgccatttg cagttccaac agctttttca ctttgaatga cagactctcg cgagtgtcgt
     2221 cattatcagc tttgaaggcg agtccgagga taccgatttt gcgccccttc aggttgccca
     2281 ttttctcctc caactgttgc acaaggaagt tcggcagccc ttcgttgacc agcatggcgg
     2341 agtgtccgag aaaaaactca ttgctgtgaa aggccgagag ttgcatcgta tccttgaata
     2401 agcaggggcc tgcagcgagc ccggcggacg caaaattttt tgcgcgggga tagtcatcct
     2461 tcatggcgtt aaagatcttg tagaagtcga accccttctt ttcaacgatc atgtagaatt
     2521 ggttggcgat ggcgaattcc agataacgcc aggcgttcgt catgagtttc gccagttccg
     2581 cctcggccgt ggagagcgga acaatttttt tagcgatggc tgaaaagagt tcagaggctt
     2641 cttgctcagc ctgtgctgag gtggccgaaa cgatttgagg gaggttgaag atttcctcaa
     2701 tccctttgcc ttggacgatg cgttccggac aaaaagctaa cttagaactg ccgagtgcct
     2761 gcacaagcag atgatcgacg atttgggtaa cgccagggaa gatggtgctt cgcaagatga
     2821 caagctggct tttgttcatg aaacgaaggt aggatgtgac aactttgagc acgtcattga
     2881 cacgcggatt caagtgttcg tccaccggag ttccggtgac aaaaacgatg acatcttgtt
     2941 gggctgcaag cgaaacgtcg gtagtggcaa aaagattttt gttgatatgg gcatgcagca
     3001 actcttcggc cccttcctct ttgaaaggca ggcttcctct tgacacctgt tcgaccgctg
     3061 tttgattgca gtcaattaac gtgaccggaa aaccccggct tgcaaatgcc agcccgagcg
     3121 gaataccgac atggccacat ccgccaatga tacatatacg cgttgtcata tggggtatat
     3181 tacttcaacc gggttaggtt gtctatccgg gttatttggt gtattgtgaa tccacgcctt
     3241 tccttttcat gagggtaggc ggactgtggc cttcaggggc ggagaggccc ggaaggcggg
     3301 tgcggacgtt tttcgaagaa tatgaatggg atgcaaacat ggcagcggac gcgtttggcc
     3361 gctgggcagg acgagcgata tgtgccttcg gcgtgcacgg aggtcacggc gctggcctat
     3421 catttctggg acagcgaacg gttcgacgag cagttcgcgt ttctcgaatg cgcgatccgg
     3481 gagacgtggc ggtggtgcgg cttgctgaag acggtgctgg tggtgaaccg ggtgaccccc
     3541 cggattgagg cgttcgcgga ggcgttctcc ggatgggtcc ggtttgacgt gttggaaacg
     3601 ctgatccccg gcaatctcca ttccatgagt gcggattgta atgcgcatct gcaccggcgg
     3661 ttcgacacgc cgtatgttct ggtggtacaa aatgacgggt tcccgatccg gagcggactg
     3721 gatgcgtttg tcggggtatg ggattttatc ggcgcaccct atgtgcgcaa cacctggtgt
     3781 aatcgcatcg tctgcgggat gctgaactgc tgggttagta atggtgggtt ctccttgcgt
     3841 tcgaagcgga tctgcgagtt ggccgcccag tactggcgga aaaagtactg tgcgctgccg
     3901 gcctcccgaa tggtttcgga ggacgttttt ttcacggaaa cgctaccgtt gcgggaacgc
     3961 gcctaccggc gtgccgtacg catcgcagcg tatcccgagg cggtccgctt ctcctatgat
     4021 gccgccttcc cgtacactga acccgtgccg cccttcgggt ttcacggtct gaaagccttt
     4081 gaatttctgc gtcagaccgg gtggatatcc gatgcggggg acacgtctgc cggcccggaa
     4141 tatgcgccga accgtccagc gcggtaatcc cggtatccgc gccagaacag gctcagccgt
     4201 tcccgccttt ggggttcgac cgtcagcgct ttcaggagta gggtcagggt gttgcgcttg
     4261 tccgcacgga tgaacgcgga cggttcggga aaggcgcgcc agaaatacaa cccgttacgc
     4321 atgatgtagt atgagcggac cggggggtgc tggttcacgg tgatctccct cccgaaaagg
     4381 cggcggtgtt cggcgttgcc gatcctgtga atcagccacg cgtccggaca ctggacgacc
     4441 cgccacccgt tccaccgcag tcggaaacag aattcgtgat ccacgtaatc gatgaacagt
     4501 gcttcgttaa accctccgac cgcctgccag gcctccagac aaatcatgca tcccgaggtg
     4561 atggcggtct ccacctcacg ccagtttgcg atcggtgcgg acgtgttttc ggagggtgtt
     4621 ttgtccgcag acacggtctg cggcgcgaga acaccgattt gcgaccgctg ttctcgcggc
     4681 attgcctccc atgcggcaat cagcgcggca accattccgg atgtcaggcg gctatcctga
     4741 tcgagcgtca ggacccaatg tgccttgccg acttgggcgg tgcgcatgcc gatattcaag
     4801 gctgcggcga cacccagatt aacggggttg aaaatcaggg tgaaccggtc ggtatgcggg
     4861 gcgaaggctt gagtcagaag gttacgcgca gactcagttg cactgttatc gaccacaatt
     4921 acatgatctg cctgctctgc cgcatcccgc acagtgtcga gcacacggtc ggaagggtga
     4981 taggtgatga ggatggcgtg gattgggcgc ataggcggaa cgacgcagag gccggttcgt
     5041 tcgtcagtgg tgagacggcg ggttcccgtc acctccatcg ctttgttggg gtctggtctc
     5101 atccgaattg aattcaaacc taacatcaat atcaatatac cataacatgt ctcttacggg
     5161 gatgacagcg tttttcggct atggtatagt ctaacgtgat cgggaggagg gggtttcaat
     5221 tggagcggtg ctgtggtggg cgaggaaacg gcaaacctgt tcgaatggca gcgtaatcgg
     5281 ctcgaagtgc agcggtcggc cgtctttgga gcgccgcagt catctcaggt tacggtggtg
     5341 acatatcact tttgggatgt ggaacaggct gacctgcgtt ttgatgctgt ggagtgcgcg
     5401 ctgtacgaaa cgtggcggca ttgcgggaaa ctaaaggcgt gtgtggtcgt caaccgggag
     5461 accccgcgcc tgcgggcctt tgcggaggca cagggagatg cggtgacgat ccgggtcaat
     5521 cccgcgctga ttccgggcga tcttccttcg atgagtgtgg attgcattgt caacctgcac
     5581 cggtattttg atacggattc cgttctggtc atccagaatg acggttttcc cctgcggggc
     5641 gggatcaagg cattcgaagg ccgttacgac tacatcggcg cgccctttgt acgcgacacg
     5701 gcccttaatc ggataatggg gctgtggccg cgatttgccg tgggcaatgg ggggttctca
     5761 ctacgaacga aacgaatctg cgagatggcc tccttctact ggtcgaagcg gtggtatcac
     5821 cacatgccgc gggattggcg gtttgtgagg gaggacgcct tttacggtgt cctgctgcca
     5881 tggttggaga cgtcatatcg gcggacgatc cggttcgcac cggtggacgt tgcgctgcgg
     5941 ttctcttacg acgacctgta tggcgcatgc ccggctgaac tgccgttcgg atttcacggt
     6001 tcggctgcct ttcgggagtt tgtggagaag gggctgctga agcagggcga acagggagcg
     6061 gcggcggtca gccctttttg acggcgcgca ggcagagcag ccccacgcat acgcgctcgg
     6121 cccagaccgc caacaggagg aacgggtgcc gcagggcctg ccgccatttg ccgttctcca
     6181 aaaacacggt gacaaagcga taccagaatc caaactgccg ccgggtcacc gggtcccaac
     6241 cccacttttt gatgtaggcg gagaaggatc cggaatagta ccgtttcttg cggagatggc
     6301 gtcggaaggt cagccgttcc tcgtggtgaa gcagaaatcc gtttgtcagg gcgatgtttt
     6361 cggtgagggc cagaatccgg cggtccagat cccaatcctc gcaggcgaac agggtttcgt
     6421 catacccgcc tgttgtttcc agcagggcac gccggaacac gcgcaggccg tcaatgcagg
     6481 tcgcgttgta aaacccgcgt tcgaaattcc gcacgcgagt ccaccagcct ccgccggtgc
     6541 ggcgttcccg gatgtagagg gcgtcgggcg gttgcggaga ggtgaggcac gtgtggattt
     6601 ccgccagggt ctccggcgga agggacatgt cggcatccat gaacaacaca aacggggcca
     6661 ctgcctcgcg ccagcctcga ttccgctgcg tgctgcgctc ggggccttgg ctgaacaccc
     6721 gggcaccgag tgtggcggcg agttgcggcg ttccgtcatc ggagttatta tcgaccacaa
     6781 ggacttcgca ccaacctgcg gcaacagcgt cggaaaacga ttcgacgcac gcggtgagat
     6841 tccgcgattc gttgcgggtc gggatgatga cacttagcag aaatgtaccc ataacgcgta
     6901 accaagccct ctcttttttc gtaatacgta tgatggcaga atcataccgg aaaacaagat
     6961 tgatttacgg ggcgtgcggg tccggttggc cgggcttagg cgcgtgagag acgccggatg
     7021 gcggttgccg ggatcgcgcc ctcacgcagc acggtgagtg tgccgtccgc ctctgctttc
     7081 accaccgtgc tgggagtgcc gcccggcgag aggccgtcat ccagtacccg atccgccaca
     7141 agccccacat cctgaagcgc ctgggcggcg ctcgtcgcgg gcattgcgcc gctgaggttg
     7201 gcactggtga cgcgcagggt gccgccgcac gcagccagca gcgcgcgtgt gaaggcgtgg
     7261 tccggaaccc ggaatccctc ccagcgttcg ccgcacgcca gaaccagcgt cagcgcgccg
     7321 ggccagcagg cgtccgccag acgccgggcc ccttccggga aatgggcgcc gaacgcgcgc
     7381 accgcgccga catccgcggc gagcaaggcg ataggcttgt cttccgcccg ccttttaatg
     7441 gccgaaagcc gtgccaccgc ttccgggcga tccggatggg ccgcaatccc gtatacggtg
     7501 tcggtcggga tcacggcgat cccgccggcg cacaggaggc gcgccgcctc ggacaggcaa
     7561 tcgggcgtgg cgcgcaggac aggagggagg gggtttccgg aagagggggg tgggtgcatc
     7621 atgttgttca gacacggttc aggcgcagga gcggatcggg atgccggatt tgatttatca
     7681 tacttgaacc ggaagagcaa ggggggtatc atgtgttttg cggtcggctg atcatcggag
     7741 acatgcggga gaagcgcgtt tcagaggaag gttgatatgg aagagaaagt gttggtgaca
     7801 gggggcagcg gctttctggg aatcaatctg atccgttatt tgaaaggtaa gggggtcacc
     7861 gggatccgtt cgatcgatct cgagccgttc gattacccgg agaaggcgga agtggatgcc
     7921 gtggtgggcg acatccggga tgtcgaggcg ttgcggaagg tgatggaggg ggtcacgtgg
     7981 gtcgtgcata cggctgcggc gttgccgctg tacaccgagc aggacatcat gaccacggat
     8041 gtggacggga cacggaatgt catgactctg gcccgggagg ccggggtccg gcgcgccgtc
     8101 atgatctcct cgaccgccgt gtacgggata cccgatcacc atccgcttgt ggaaaccgac
     8161 cggctgatcg gcgttggccc ctatggccgt gccaagattc tggctgagga ggtatgcctc
     8221 gaagcccgtc agcagggcct ttgcgtgccg atcatccggc ccaaatcctt tgtcggtccg
     8281 gaacggctcg gcgtgttcgc gctgctctat gactgggcct ataccggaca cgggttcccg
     8341 atgatcggca gcggcaagaa ccgctatcag ttgctcgatg tggaggatct gtgcgaggcg
     8401 atctggctga cgctgacccg ggacgaggag gtggtgaacg acacgttcaa catcggcgcc
     8461 aaaatcttcg gaaccatgcg cgaggattac caggccgtgc tggatgaggc gggttacggt
     8521 aaacggatcc gcgggttccc tgccgcgccg atgatctggg ccctgcgctt tttggaatgc
     8581 ctgcgcctgt ctcccctgta caagtgggtg tacgagacgg catgcgaaga ttcgttcgtg
     8641 agcattgaaa aggccgaacg tgttctggga tacgcgccga aattttcgaa ccgggacgcg
     8701 ctggtgcgga attaccgctg gtatgtggcg aaccgggaga ctttcaaaaa tgcgtcaggg
     8761 gtttcgcacc gcgtgccgtg gaagcagggg attctgcgtt tcgcgaagat ttttttctga
     8821 tgggagcggc aagtgtctgc ttgcccgtga gttgagtaag gactcccgag ccggcggcta
     8881 aacggacggt tcccgcacgg ctcctgttcg ccaaaaacgt gctgcggttt tccggggttg
     8941 actcttttcg cctgcccccg cataatgtca ttcaatcgta agctctggta tttaacagga
     9001 caggaaggtc gtgatatgaa acgaatgttt tgtgcgcttg ttacgggtgt tttgtgcgcg
     9061 gcgggcggcg tgaatgcggc gaacacgttc accttgtgga ccggggccga cgggacggat
     9121 ccgacggacc tcgcccgacc ggcgaactgg gagggcggcg ccgtgccggt gatcgacacg
     9181 ggaacgatgg acggcccgtt cggaaagttc tattcgctgg gcacagtgac cgtcccgctc
     9241 acgttgagcg aggatctctt cttgaacggt ctcgtcttcg attccgtcgg gaccgccttc
     9301 gatctcgatc tgacggggcg gacactccgg ctctacggct actcgcagga ctcgcattcc
     9361 ctcacgtttc gcacatttcg caacgttccg gagacgatcc gcgtgcgggg cggcacgctc
     9421 gatatgacgg acggcaaaca gaagaccgtg tacgtcgacg gtgcgagcga cgtttcgttt
     9481 tccggtacgg aactgcgggg gggcatccag ttccgtacgc tttacggcgc gaacgtctgc
     9541 ctcacgaacg gcgtcacggg ttccgcgtat ttcacgacgt tcgcgggcac gaaaaccgtg
     9601 atcgcgggca cgggcacgga aatcatcggc gcgggcacat gctcgatcgg caacacggcg
     9661 ggcgagacgg gcgacgtgtt ccacgttctc gacggcgcgt attggacgaa cgccaacgcc
     9721 tgcgtgatcg gatatgccgg gtacggcaat tacgggctta tctccaatgc gacggtgtac
     9781 gtcacgggcg gcaatctggg cgcgggcagt aacggtcagt acgcctcgaa taacgttttt
     9841 gaggtgaagg gcggggcgcg tgtgcacgta aggggctcca acggtaagtc ccttatcaac
     9901 cacggccggt gggggtacaa caacacgctc cgcatcgcgg gtgaagggac cttcgtcgac
     9961 tggtggacaa acgatcacaa tgatggttcg gttgtcgtgg gtgaaatggc catgaacaac
    10021 cttctccacg tcacggacgg cgcgcgtctc ggaacgaacg ggtgcctcgg cgtcggtttc
    10081 gtggggaaca tctattacgg caattggcgc ggcgattcga tcgggaacac gctcaaggtg
    10141 gacggcgggg ctcaatgtta catggctaac ctctgcgtgg gcggactctg caccggacgg
    10201 atggatccct acgatggtac aaacggccgt aacacctaca aacgcgggcc gaacggcgag
    10261 tggtggcaga acacctattt cgacctttcg atctcctcga acgcgttcat cgtttcgggc
    10321 agcgcgaccc gtgcgcaact ggcgggctcg cttcaggtgg cggggaaaac gaacgaggga
    10381 ccttgcctcg cctatgtgca cgagaacgat ttcaaccgtg tacgcgtcga ggacggcgcc
    10441 gagctgaccg tcggatccgt gagcctgggc aagtgggggg tctcgaacca catcgaggtg
    10501 gtcggtggtg cgctcctcag cgtcaccacg gggaatacgg gttattatga cggcttctcg
    10561 ttcggcggtt ttcccaccac cggaacgtgg gtgcgcgttt ccggcgcgaa ttcgcggctt
    10621 tccattccga attccagact tgccctgatg ggctgtccgg acggccggga gaacaagatc
    10681 gagatttcgg acggcgcgct cgtgacggcg aaacacgtca actcttacgc gacgaacaac
    10741 gttttccatc tgaacaacgg aacgcttacg gtgacggact cgctgatctg tccgaacggc
    10801 gatgcgaacg gcgcgaagcc ccgtttcatc tttgaaggcg acgcgccgcg gatacgctgc
    10861 acctccacag atgacggctg ccagttcaag acggagccga tctttgagtt caggatcccg
    10921 gagaaggggt ttgcggatgt tccggtcaag tcctcgggac atctgcgcat ctacggcgag
    10981 cccacgcttg tgctcgacgg ccggtgcctc cgctcctatt cggctgcggg tggcggcatc
    11041 caggttctgt ttgaggccgc tgaaggaaag acgctcacca tcacgtccga gcggatcgcg
    11101 aagatgagcg gggaggccgc ggccgtgctc gatggctgtg ccgtgacgct cgtggacaaa
    11161 cgcaagctcg tgctaagcgt cccggataga agcggcacgt taatccggct tcactagccc
    11221 gacgatcctt tcacgtcacg accgtcgtga ctgtcacagc gaggcacaga agcgggcgaa
    11281 gcgggtcatc ccctcctcga tttcgtccat gccgcaggcg tagctaaggc gcacgcaggt
    11341 gtcggcacca aaggccgcgc ccggaacgac cgccatgtgt tccgtctcga gcaggcgcgt
    11401 cgagaaggtc aaagagtcga ggccgaacga cgagatgtcc gggaagacgt agaaggcgcc
    11461 cgtggggatg acgcacttga cgcccgggat ggcgcgcatg cgttcgcaga tgcggcggcg
    11521 gcgggcgtca aaggcggcga ccatttcggc aacgctcgcc gcgccctgtt cgagggctgt
    11581 gatcgcgccc cactgcgcga aggtgttcgg ggcggaggcg caatggctct gcagtgcgct
    11641 catggctttc atgaacggtt caggaccggc cgtatacccc agccgccacc cggtcatgga
    11701 gtagggcttg ctgaagccgt tgaccgtgat ggtgttgtcg tagagcgctt tggagaagga
    11761 tgccatgctg acttggcgct ggccgtcgta gaccatcttc tcgtaaatct cgtccgagat
    11821 gatcgtgatg tcgtgcagga gagcggtctc gcccagagcc cgcaactctt cgggcgtgta
    11881 catcacgccg gtcgggttac tggggctgtt gatcaccaag gctttggtgg cggggccgat
    11941 caagcgctcg atgtgctggg gcgtgatttt aaaagactgg tcggccgtgg tgtggatgaa
    12001 aaccggtatg cctcccgcca cgcggaccat ctccgggtag ctgagccaga acggtgtggg
    12061 gatcagcacc tcgtcaccgg ggttgagcag cgtctgaaaa accagcgcga gcgagtgctt
    12121 gccgccgcag ctcaccacga tctgcgaggg tttgtagtca aggccgttgt cgcgcgcgaa
    12181 tttggcggaa agcgccttgc agagttgggg cacgcccgcg accggcgtgt acttcgtctt
    12241 gccctgccgg agggccgtga tggcggcttc tttgatcaga tccggcgtat cgaagtccgg
    12301 ttcgccggcc gagaagttgc agacgtggca tccctgttcg gccatctgtt tggccagact
    12361 ggagatggcg agggtttggg agggtttgat ggcgaggatc gcgtcgttca gttttttcat
    12421 gggtagtagt tttgaatcag ctcttcaaag tgcgggtcgc gggccacatc gatgatccgc
    12481 atctcgcgat gggcgttctc gacggaatct gacgcgtgcg cggcattgac catgatgttg
    12541 gtgccgaact cgcggcggat cgagccggga cgcgccttga gggggtctgt cgcaccgaga
    12601 atatcgcgga ttttgctgac ggcgtcaata ccctcgtaca cgatcgcgag gcactcctcg
    12661 gtgccgggca gggtgcgttc ctcgggcaga cagttcgaag ggcgacgtcc ggtcatgaac
    12721 tccacgatac tctcgaactc gcgctcggca aaggagggcg ccagcatgtc gcacagacca
    12781 tcgagcggag cggcatccac cgggaagccg aattcgcgcg acaacgcctg cgcggcacga
    12841 tccgccgcaa accgcttgaa aaccttcttg agcccctcac gcaccggccc gtaaaactcc
    12901 tcggcctgcg ccaccgacat ggagaacttc ttcacgccca cgatgcgcaa cccggagttc
    12961 gagagcaggt ccatgatgtt gcccgcgcgc agcgaaggct gacgccagtt gtccggcttg
    13021 agcatcacca gcgtctcctg cgcgtcagcg tcgcccggca cgtccagcgc accacgcacg
    13081 atgccggcct gtgacgcgag atgcggcagc cacatgcgca ggatgtcgcc cacgaccttc
    13141 tgggaggggc cgaccaacac cgccggctca aaatactcga cctcgccggt cgccgggttg
    13201 cgcacaaggt cgccgtaggt gtcgcgcacg ctctgcccgc tgcaccattg ttggcggatc
    13261 gagccggtga catcgaagat cttgcggatc gcgtcgtctc cctcgaagag cagcaggatt
    13321 gaacggtgcg gccgtccggt cgcggcgtcc ggcatgtgga agcgccgtac gtagttcgca
    13381 aggagcgcgc acttttccgg gtcgtcggga tcgtaggtct ccagataggt ggcgaacgca
    13441 ttgaccagtt cggccgacgc accgatgacg cgcgcgccga tcagcttgag atcggtgcgg
    13501 acgagataac gcgcgatgat gccgccggta cgcgatttcc ggatcgaata cggattgatg
    13561 aggacgaacg ccagttcctt cgccatgtct acactttcct ttcactctgc ctcatggtct
    13621 gccggatgct gacagccccc atcacgatca acgcgaccga catcacgatc aggatgccgg
    13681 tcgtcaacgt catgacgcct ccggccttga agccgtccgc ctgccaagcc tgcgcattgc
    13741 gcaggaagag ggtaacgatc gcagcgccgc tggtggccat cataaagagc atcggcaaca
    13801 gcgtgagggc gagcgggcgc ttgtggcgca acagccacag actgcaaccg agcagcgtca
    13861 gcgcggcgag caactgattg gacgaggcaa agaccggcca gatgctcttc gccccgccgc
    13921 cgagcagcaa ccagcctgcg gcgaccaccg caatcagcgt ggcgatatag cggttagaga
    13981 agaacgcatg ggacgcgggt agcgtcggcg cgacagtagc cggaggcacg tctcccgatt
    14041 gcccgcgcgg caggaaaagc tcttgccaag tgaagcgggc caggcgcgtg gcggtatcga
    14101 gcgtcgtcat gagaaaagcc gccagcgcca gcgagataaa gatgatgccc gcctcgtgcg
    14161 gcaaccccag tttgtcggtg aagctcgcga cgccgccagc gaacagctta accgcgccct
    14221 gctccttcag cgccaccgtg tactcgcctt gcgtcatcac gccaaccgcg accagcgcaa
    14281 tgatcgccag caccccttcg atcagcatgc cgccgtaagc aaccggccgg aggtggcgtt
    14341 cgttgtcgat ctgcttggcg gtcgtgcccg aggccaccag cgcgtggaaa ccggagcagg
    14401 cgccgcacgc cacagtcacg aacagcagcg ggatcagagg atcggagcgg ccgctcaacg
    14461 gactcaccgc atgccagccg gtgaaggcgt caagattcaa cgccggtcgc gccaccagaa
    14521 taccaacgaa accgagtgcc atcatggcat aaagcagata ggaattgagg taatcgcgcg
    14581 gttgcaacag cagccacacc ggcagcaccg aggccgcgaa gcagtagacc agcagcacca
    14641 ccacccagat ctgccgggcg accgccgcat cacaacccag cagcttcacc agatcaagcg
    14701 ggatcagcgt gccgaaatag acgcaggcaa aggtcagcgg cacaaagatc agcgatccgg
    14761 tcaggacact catgccgccc cggtagacca gacggccaaa gccgaccgcc aacgcaatga
    14821 acagcatcga ggccgtgccg accgccggaa tctcgacaaa cgtttcggcc acgatacgca
    14881 gaaactccgc cacaaccaga accagcgtga accagcagaa aaagaggaag agcatccgcc
    14941 cccaatatcc catcagcgac tcgatcaccg taccgatcga acggccttgg tgacggatcg
    15001 aaaggaatat cgcggccaga tcatgcaccg cgccgatgaa gatgcagccc agcacgaccc
    15061 agagcgcgac cgccacccag ccgaactgcg ccgccaacac cggcccgacg atcggccctg
    15121 cgcccgcgat gctcgcaaag tggtgcccga agaggaccgc cggatgggtc ggcacgtaat
    15181 cgatgccgtc tctcaatgtg tgcgcggggg tgggtcgttc aggatccacc cccagaatac
    15241 gttcgatgag gcgcccgtac atggtgtacg cgagcagaaa aagaaccccg cagagactca
    15301 gtaataacgc gccattcata tggaaaactc ccgtgtggag cggttttgaa tcggtatcgt
    15361 accgcttcgg aacaggggtg tcaacgaaag gtgtcgcagc cacgccagct cgcggcgccg
    15421 catgcgcgcc cgttctgcgg gcgtgcggtc ctccgcgccg gccaggtgat cgaagccgtg
    15481 cgcgaggtag agcgccagtt cgcggtcggc attccagcct gcgcggcgcg gcgcggcgcg
    15541 cgcggcgcgt tcgacgttca cgaacagttc gccgatcagg ccatcgggtt cgccggggat
    15601 cggctcgtag cgctgcgtga tgacatcggt ggggcccgca tgcttcagga tcgcggcgtt
    15661 caccggtgtg atgcccgcgt cgtccagcag gtggatcgtg atctcccgcc agacggggcc
    15721 gtttcgcaaa cgcgtctgcg cccgcttcgc aagtgcggcg gccaacctct tgagggctgc
    15781 ggtgtcgagc cggaaacggc gcgtaccgat tcggatgtaa atgttggggg aggacatgat
    15841 gggtcgggac cgtccgcgtt gccggggcac ggtcccgaaa aggctcagac tcgatagtac
    15901 tgttcccacc ctttgcgcgg gggcggcccg tccagcaggt cgttggcgag tttgttgccg
    15961 gtgatccgcc gcgtgaccgg atccagggtg aacgtggtgt tgagacgctg ggcgatgcac
    16021 cccagcgcca gcacctgact gagggggccc gcgacctcaa aggaggaacg gcacgtttcc
    16081 cggcccatac acgccagcag gaagttcttg tagtggttcg actggttttt cggatattcc
    16141 ggcaggcggt tggccatgtc gcgcgccttt tccgcgggga tgatctcaag cgtgctgctg
    16201 tgcgagcctc ccttgaaggt caggtcttcg gcgtagatct ccttgcccgg actcaacttg
    16261 ctgaccgccg tcgagccgcc gcccggggtg gggatgtcat ccgcggtttt ggccgagccg
    16321 ccgaaccccg gcggcaattc cgggaagttc ttcacaccgt cgtaccacag gacttcgacc
    16381 ggcggcattg cgccgcgcgc cgcgaacttg aaggagagcg tggaagccat cgggaatacg
    16441 taggggttgt ggccttccag attgagggcg ctcacttcgg tcggcatccc cagttccaga
    16501 aactggtggg ccgtgtccag aatgtgcgcg ccccagtcgc cgagcgcgcc gttgccgaag
    16561 ctgtaccagc agcgccagtc gccatgcacg taatcgcggt gatagccgtg gaagggcacc
    16621 tgtcccagcc aggtgtccca gtccagcgtc tcgggcacgg gctgggccgg ccgcaggctt
    16681 gtgacaacgc cgttccattt gtgccagcgc cgcgcgccgt tcatgtgggc aacgatccgc
    16741 gtcacgttcc ggatgatgcc ggcctcggtc cacgccttga actggaagta gttcgcgccg
    16801 gagtggcctt ggttgcccat ctgcgcggcg acccggttct ttttttcggc cttcatcatc
    16861 aactcgatct cacggaaggt gtgggccatc ggcttctcga cgtacacgtg cttgcccagc
    16921 cccatcgcca tcatggcgat cgggaaatgc gtgtgatccg gcacgccgat gcacagcgcg
    16981 tcgatctgcc cgcccagttt gtccagcatc ttccggaaat cctggaaccg cggcgcgtcc
    17041 ggaaacgtat cgaggatttt ccgcgtgtgg ggcgcgccca tatcggtgtc gcacaaggcc
    17101 acgatgtcgc acaggccggt cgcgtacagg ttgttgatgt cgctggcccc ctgattgccg
    17161 atgccgcagc aggcgagtcg gaccttgtcg ccggtcacgg gccggcctgt ggcgcggatc
    17221 gcgggaaagg cggtcaaggt cgaggcggcc agggtgcgtt tcaggaagcc gcggcgcgtg
    17281 gcggccttgc ggtgaggaag tgcggtcttc gttcggctca tcatagtctc tccttgtgcg
    17341 cgtcgtgttc acagacggtg tagaacagag aatataacct tccgttatgt aaagtaaagc
    17401 ccgggaatac gatcgcgggt ggcgcggggg ttgccgcgtc cgtcgctcac ccgtcgatgg
    17461 tcgcttccca gggaggcgtc tcaatggcct ttttgccatc atctagcaac aggtcggatt
    17521 gtttcgtgta gttgaattgc tcgccccaga aatcgctcct tagcttttca tcgaatatct
    17581 caaaagcacg ttcggcctct ttttctttga aaaacgcctt gtaagcgtct gcggggaaag
    17641 ggccaacatg cgaaaaatag acattcccgc cataacgttt ataaagtgct ttgttccgtt
    17701 tccatagaag tacgttcgtt cttaatacat catctgcagg cacgaaaaaa ctaggctcca
    17761 gccatgccat ttctttcctg atcttttcgg ttttgttcga gacgtctaca gttccgtcgc
    17821 cagaaacgtt gagcatttcc aactctttgt taaggtatgt tatccgttgg tcataaatca
    17881 tcttggccag cttcacgaag aattcgcgtt gtttttcaat ctcttgcggc gagaccgcaa
    17941 tgttttcatc tttgtcgaac tgctccagca gtgttttcac gatgatggac agggcgtcgt
    18001 tcgtttcgct tatcgcagcc ccgcccaccg ttgcgaccgt gttcattttt tcatgcttag
    18061 cgtttaacat gcttaacgcg actgtaagcg tgagaacggc cacaaaaacg taaacaatat
    18121 ataacagttt tttcatgacg ggggagcttt ccttaatgat tatcggggtc gtagtttgac
    18181 attggacatt tcatttttcc agcaccctca atgcgtgcgc cgtcgaccga cacaagtctt
    18241 gttggttttc agccgagact ccacccttcc ggtgatgcct cagttgcctt gccacggcgg
    18301 tacattagca caccgcgaaa acgctccgca acccttggaa tcggttagtg accgacgcag
    18361 ggatgtgcga agatcatgcg tttctagtga aaaggggagc ggagagtcgg gcgcgcgggc
    18421 gtccccgccc gcaacgacgt gtcttgtgct ttctggtttt atccgcagat ttacacagat
    18481 ttcgcagatt t
//
LOCUS       DAOSRB010000035        18312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3194_length_18312_cov_7.559128, whole genome shotgun sequence.
ACCESSION   DAOSRB010000035 DAOSRB010000000
VERSION     DAOSRB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18312
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3194_length_18312_cov_7.559128"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..2099)
                     /locus_tag="PLZ74_03355"
     CDS             complement(237..2099)
                     /locus_tag="PLZ74_03355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOR97391.1"
                     /translation="MSGLWTEDRLKQELSDRFGYAAFRAGQGEAVRTVLDGRDLVLVM
                     PTGSGKSLCYQLAALLLPGVTVVVSPLIALMKDQVDALDRKGIPATFLNSSVDSAEMA
                     YRLDGLRQGRYKLLYVAPERFRNRRFTEAFAAVQVALFTVDEAHCISQWGHDFRPDYL
                     NLKHVVERLGAVRVLAVTATATPDVREDIVRQLGLGVAPRQAPEVRVTGFARPNLHLA
                     VTRCATHRHKFTRLVQMVEAHRCGIVYCSTRKMAERVAARLESEGLAPITYHGAMADG
                     ERTAAQNRFISEPSPVVVATNAFGMGVDRSDLRFVTHWDIPGSLEAYYQEVGRAGRDG
                     ADAWCELLFNYADVGTQRFFLDGANPPFDDVLAVWQTVRKACESAPVTWSVERWAGEA
                     GLGNEMAARTILGLIERARLIEREILPGQRTYTTRLLPNPDPALKELKRLTAGLEEKR
                     RRDERKLETLLRYVDHPGCRHAFMLNYFGDQTSSSSCSACDRCQYRAPHPPTELTEEQ
                     WIEVQKVLSCVTRMKGRFGAQRIAQVLRGDSNEVLTRHALDQLSTYGLLPDKSVAQIR
                     AILDALHGAECVAVTPDTYRLVSITPKGHEVMMRRLKGFRLAWPQKGQRSRRYY"
     gene            complement(2124..3137)
                     /locus_tag="PLZ74_03360"
     CDS             complement(2124..3137)
                     /locus_tag="PLZ74_03360"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOR97392.1"
                     /translation="MQNIDWSNLGFKYMDTCCHIRYVWRQGAWDEGELVSDPWLKMHI
                     AASCLHYGQAAFEGLKAFHCQDGRVRIFRPEANAERMANTARRTCMAQVPVAMFTEAV
                     RRVVKANEDYVPPFGTGGSLYIRPLLIGSGAQIGVAPADEYTFLVMVMPVGPYYKGGL
                     KPVRALILDDYDRAAPQGMGDVKVAGNYAASLYAHESAKHAGYPVELYLDAKTHTLVE
                     EFATSNFIGIKPDGTYVTPDSCSILPSVTNNTLQQIAKDLGMSVEARAVPYDELPEFS
                     EIAACGTAVVITPVYEITRGDRVIRVGAPDGCGPVLQKLYDTVQGIQYGVLPDRHGWC
                     VEV"
     gene            3332..4387
                     /gene="argC"
                     /locus_tag="PLZ74_03365"
     CDS             3332..4387
                     /gene="argC"
                     /locus_tag="PLZ74_03365"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOR97393.1"
                     /translation="MIRVKVVGATGYGGVGITELVLQHPKAKLVSLIAREAVGQPLSA
                     VYPHLAGFCDLPVLAAEDPRAQEPADVVFFATPDRVGMCGAPEELARGAKVIDYSGDF
                     RFTSAEAYADYATRIGKDPVHAAPELLGRTVYGLAELHRDAIRTSTLVGNPGCFACSC
                     ILGMAPAVRERLLMPDTIICDCKTGVSGAGKKVNAAHHYPARYEQMNAYKLAGHQHVC
                     EVERELGLLAGQEVRITFTAQVVPVCRGILSTLYGTLTHAMPEAELVALYRDFFRESP
                     FVRVFDSQTAIGTLNVRGTNFCNLVVSVDARVNRLRVVAHIDNLMKGQAGSALQNMNL
                     MFGLPETTGLDRPGIYP"
     gene            4384..5424
                     /locus_tag="PLZ74_03370"
     CDS             4384..5424
                     /locus_tag="PLZ74_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOR97394.1"
                     /translation="MSNKKSAPKKVVAAAGRKTAPAASRKHPKPGLGRGLGALISRPS
                     GNAVQTPNVAARMDAPERTGYDTAAPDPRRSVLDVPVAEISRSPWQPRQTFDAESLSE
                     LADSIRANGVIQPLICRKTGDARYELVAGERRLRAAIEAKLATVPVILVEAEDRRAAE
                     LAIIENIQRQDLNAIEEAEGYHTLAETFNLTQQEVADRVGKARASVANALRLLDLPDE
                     VKQLISSGLLSTGHAKVLLSLTDVTEQTLLGRKAVTEGLTVRALEKVVQRRNKRDAGA
                     TLRRPGQPDLPESYVRDLTDRLHKHFGTAIRLSSSVTHANGKHTKGILEIDFYDNDDL
                     DRLLTLFGITLD"
     gene            5453..6391
                     /locus_tag="PLZ74_03375"
     CDS             5453..6391
                     /locus_tag="PLZ74_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOR97395.1"
                     /translation="MRRMMLGWVLLGLTGCDLGTTNPEPVSAPPPQVWTAARASNAFA
                     YVEGLVRDCTPRDAGTPGGEKAACWIRDRLAEQGLPARIDRFTEPTPRGPREFRNVLA
                     TLPGTGDTWIILLSHFDTMSGIGEGFQGANDSGSSTGLLLELAGALRDAGPREPSILF
                     GFMDGEECMIAYGAQDGFHGSKRLARQMKEEKRKIRAVILTDMVGDRDLKLTLPRNST
                     AALRVLALEAAEAVGLRDHIGLFDGNIYDDHQAFLDLGYPAVNLIDFSYGSRPGLNDY
                     WHTRQDTLDKLSVESLRITGAIVLEMVNRLSSPARR"
     gene            complement(6413..7459)
                     /locus_tag="PLZ74_03380"
     CDS             complement(6413..7459)
                     /locus_tag="PLZ74_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOR97396.1"
                     /translation="MDGQFDANADTTMEVRFGDTPPEPMPNRIDVARDPAVGQFVRYL
                     TAERNASAHTVAGYLQDIGQYAAFVWGTEKRGGPPFAWDEPDRARARAFLSAFHRKGH
                     APATTRRKLSSLRTFYRYLEREGRTTAQPFAGLRGPRLGKRLPVVLSLREVEALLAAP
                     LAALRKRSDAGRAPSLWAEYAALRDTAIFEVLYSTGCRIGEVAALVWRDIRFESGTTI
                     VEGKGRKQRLCVLGRPACDALIRLRDAATRLWPATAGPQAPLFLNREGGALTSRSIER
                     QMKVWLRAAGLPQELTPHKLRHSFATHLLNAGADMRSVQEMLGHASLSTTQIYTHVSI
                     DRLKDAYAKAHPRA"
     gene            complement(7506..10073)
                     /gene="topA"
                     /locus_tag="PLZ74_03385"
     CDS             complement(7506..10073)
                     /gene="topA"
                     /locus_tag="PLZ74_03385"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOR97397.1"
                     /translation="MSKKLVIVESPAKAKTIGKMLGSGYVVKSSVGHIRDLPERTLGV
                     DIEQGFVPKYVLSKGKTKVIAELKKAVKGCDEIYLAPDPDREGEAIAWHLRETLAEVA
                     DGKPFHRVMYNEITPRAVRAAFEKPGHLDMRRVDAQQARRVLDRIVGYMVSPLLWRRL
                     KRGLSAGRVQSVALRLLSERERQIQAFRPEEYWVMGARVRRALAPLDPFTVKLAKVDG
                     EKPVIGSETEARALLSDLEGCTLRVREVRVRETTRRPLPPFITSTLQQAASTVCGFSP
                     SRTMSLAQKLYEGVALDAGGPVGLITYMRTDSVNVSRDAQAAAREHIITAYGKEYYPA
                     SPNVYRSRASAQEAHEAIRPTEVTRTPESLKGILDASSLKLYDLIWRRFVASQMAAAK
                     IAQKTVEIEPVPPASTGVAPRRACLFTASASEVRFDGFLKVMALDIRRKKPEEEDPEE
                     EVEEVQRLPPLAKGDGLVPLEWLSERKETKPPARYSEASLIRALEANGVGRPSTYASI
                     LETLSSRDYTTREKRQLMPTPLGLEVNDLLVGKLGSLFDVGFTARMEERLDEIEAGGI
                     GWTAMLDDFYKRFEQWMEQAKEPPADAGKVETMLSLLERVTVWAPAVQRGNSKRVYSD
                     ERFVKSVREQIEKSARPVSEKQLTALAKIAVRYREQLPEVEAVLKSCGFGEIVETDNA
                     APSGEEVMRRFAVLKEVELSERQMKFVESLRHQAESGRKLSEKQMAALDRIVVQNAAQ
                     IRDFETVKASLGLTAEESEPSTDHESPVLLEMLGHVTEWQAPVQRGKMSFDDQAFFVS
                     LSEQFNHKRSLSPRQRYAMRRMVFRYKAQIPGFERYAAKLGLKSKSDKTETSGTKEA"
     gene            complement(10153..11289)
                     /gene="dprA"
                     /locus_tag="PLZ74_03390"
     CDS             complement(10153..11289)
                     /gene="dprA"
                     /locus_tag="PLZ74_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOR97398.1"
                     /translation="MTEREALIALNQISGLGAVTTRRMAERFGDLSAVFGASESELLS
                     VPGIGAERARQFHAELRRVAAGPELARAEKKGVRLVTWADPEYPALLKQIADPPLVLY
                     VAGAAEALNQPAVAVIGTRRPTVYGRETARFFGYRLAAAGYVVVSGLALGIDTEAHTG
                     AVKAKGRTVAVLGGALDRLFPKENTGLAREIVAQGGAVISEYPFGRQPDRQTFPMRNR
                     IVSGLCKGVLVIEAPLNSGTLITVGQALDQNRTVMAVPGRIDSPVSQGCHRLLREGAR
                     LVTTADEVIEEVEDLLAGAKTRETPAAVRSGTEEARPPACVLSEDERKVVAQIGMEEV
                     SVDAVVRGSGLDAGTVNAMLVGLQIKRVVRLLPGGFVTRVSRAS"
     gene            11582..13225
                     /locus_tag="PLZ74_03395"
     CDS             11582..13225
                     /locus_tag="PLZ74_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97399.1"
                     /translation="MRSCLKARKPVAWFAMAALSVYGGPTSIPIRQGGVEGRPFWNKV
                     SPWFEYAPAFDFPEVNGAVKYRFIAKHQSGAEFSFEAESPQAALSPIWEKLPVGAIEL
                     RVAALDANGGFIETAPGNATVTGSAVAMKPPTAVRKFWKKATFHEGMYESAACSYAQS
                     ARKAFEFVLNEPSIRYLHDHGTPDPSYSLNGYPSKMLSAEIGILLDIAKTRPEHRAFA
                     LETAKKAADHLIRNRCAAGTPLEYFTKTYEGEGEYGRSKQNAGLNMLIYPAKAGEALV
                     KLHVALARDEGYLDAAEKIAATYLKLQGTDGTWPLKVREDTGEPVAPNRLIPLPVIDF
                     LESLYAVTKKPEYRAAANRAFAYVEKGPLKTWNWEGQFEDTKPRPPYLNPTMHEAAST
                     AIYLLKRFPGDAKRLELAMELVRWAEDQFVAWEKPEVGDRRAHHFNPANWYVPGTIEQ
                     LAMGEPIDASSAKMIEVYLALHKATGGHEYWAKARVLGDTITRAQWEDGCYKTFWVKA
                     DRDDQRYHVWMNCHIYTARVMNLLATVEHWLNGKPKRSS"
     gene            13621..14850
                     /locus_tag="PLZ74_03400"
     CDS             13621..14850
                     /locus_tag="PLZ74_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR97400.1"
                     /translation="MSDHMPMATRVKLSAMMFLQFMMLPVWFIPMFPYITEMAPKGSA
                     LPLLCGMIMGFGALASPIFGMFADRLMNSEKVLALCNFVTAVLLAYAYTIKTPGMLFV
                     TLLLVMVFYMPTWSLTATIAMANSTTEAFPQIRVFGTLGWVAAAIFSIIGTKVFGIAQ
                     FDSTPYIFLGGAIAAALGGVLAFFLPPTQPPAKGQKMSLVDTLGLRALVLLKRPDFLV
                     FSVLILLAMVPFQWYNVYNSTYLKERGYEYLTGVMNLGQVLELVFMLLIPVILKKAGY
                     KWAMVLGLLALVFRYGMFYLGAAHGLQICDYAGILIHGLIFGMLVVGSQMYVDAAAPA
                     ELRGQAQGFIGLIMFSLGTFLSNFVFDGILKKNVAADGTHVWTTPYLIAMVSALVLAV
                     LMAVAFKPAVQEKRQGA"
     gene            complement(14892..15320)
                     /gene="dut"
                     /locus_tag="PLZ74_03405"
     CDS             complement(14892..15320)
                     /gene="dut"
                     /locus_tag="PLZ74_03405"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOR97401.1"
                     /translation="MTVEFMRIHPEAVVPSYAHPGDAGMDVSACESAVLQPGERKRIR
                     TGLVVALPPGTEAQMRPRSGLALRHGVTLLNTPGTVDEGYRGEIGVILINHGDTPFEI
                     TAGMRIAQMVIAPVARAEVREVAELGGTVRGTAGFGSTGV"
     gene            complement(15510..15887)
                     /locus_tag="PLZ74_03410"
     CDS             complement(15510..15887)
                     /locus_tag="PLZ74_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOR97402.1"
                     /translation="MSLMKRDRVEAEKANRLELTPVSRITESEEAFTLTMELPGVSEK
                     QVELTLEDRTLTVTAEKEVASFKDYTLILNEIQEARYRAAFELPERVDTAKVQASCKN
                     GMLVLTLPKREEVKPRRIAITAG"
     gene            complement(15916..16317)
                     /locus_tag="PLZ74_03415"
     CDS             complement(15916..16317)
                     /locus_tag="PLZ74_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOR97403.1"
                     /translation="MRAYLSPWGNPWRVFDELMGDFPYDLFNSAWRARVGANPRVNVW
                     ESEKGLVVEAEVPGMEPDTLDVAVDANVLTLKGKRELPDGTENEFQRSFNLPFELDEK
                     GIKATVKNGVLTVTIPRKAAAEKRKIAIEKL"
     gene            16722..>18312
                     /locus_tag="PLZ74_03420"
     CDS             16722..>18312
                     /locus_tag="PLZ74_03420"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOR97404.1"
                     /translation="MSRYEQPDLILRIRKRDGRSEVFDSNKIVGAILRAGEATGEFAG
                     DEARKLTLKVMSIIHSLTDTLDLSVENVQDIVEEVLLASPYRRTAKAYIIYRDQHARI
                     REMLRKADVDLIDRYLGKADWRVRENSNMAYSLQGLNNYISSEVSKAYWLNKIYPAEI
                     REAHDNGALHLHDLNLLSVYCVGWDLQDLLRSGFKGVAGKAESLPAKHFRTALGQIVN
                     FFYTLQGEAAGAQAFSSFDTLLAPFVRYDRLNAAEVRQALQEFIFNVNVPTRVGFQTP
                     FTNLTMDLIAPSTLANEHAVIGGKEMPETYADFQHEMDMINTAFLEVMTEGDAKGRVF
                     TFPIPTYNITEDFDWERPGLEKLWEVTAKYGIPYFANFVNSDMSPDDARSMCCRLRLD
                     TRELNRRGGGLFGANPQTGSIGVVTINMPRLGYLATDEDDFLARLDNLMGIARNSLEI
                     KRKVLEQFTAQNLYPYTRFYLRQVYQRFGTYWQNHFSTIGLVGLNEACLNLFGEDIGS
                     GRGRAFALTVMDFMRARLADYQ"
BASE COUNT         3437 a         5994 c         5394 g         3487 t
ORIGIN      
        1 ggcagcaggc attccggcac gccgatatcc gagaggattg catcaatcaa cgcgcctcgg
       61 tccaccggtt cgttatctgt ccctgcaaca gtgacagtgt acggatgggg gcaccttatg
      121 ttctcgcccg ttttcataac acacttccaa tgtcgaaagt ggcacaaggt tacacgtttt
      181 caggcctttt tgccagtgct ttaactccta actcctaaat cctaaatctc ctaacttcag
      241 tagtaccgtc gcgaacgctg ccccttctgc ggccaggcga gacggaaccc tttaagacgg
      301 cgcatcatca cttcgtgtcc cttgggcgtg atggaaacca gccggtaggt gtcgggcgtg
      361 accgccacgc actctgcacc atgcagggcg tcgagtattg cgcggatttg tgccacgctc
      421 ttatcgggca acagaccgta agtcgagagc tggtctagcg cgtgccgcgt caacacctcg
      481 ttgctgtcgc cgcgcagcac ctgcgcgatg cgctgtgcgc cgaagcgtcc cttcatgcgg
      541 gtcacgcaac tcagaacctt ctggacctcg atccattgct cttcggtcaa ctcggtcggc
      601 ggatgcggcg cacggtattg gcagcggtcg caggcggaac aggatgaaga tgaagtctgg
      661 tccccgaaat agttgagcat gaaggcatgg cggcaaccgg gatgatccac ataccgaagc
      721 agggtctcaa gtttgcgctc gtcgcgccgc cgtttctcct ccaatcccgc agtaagacgt
      781 ttgagttcct tgagcgcggg atcgggattc ggcagcagac gcgtcgtata tgtgcgctgg
      841 cccggcagga tttcccgttc gatcaggcgc gcgcgttcga tcagccccag aatcgtgcgc
      901 gccgccatct cgttgccaag gccggcctca ccggcccagc gttcaaccga ccaggtcacc
      961 ggagcggatt cacaggcctt gcgcaccgtc tgccagaccg caagcacatc gtcgaacgga
     1021 gggttggccc catcaaggaa gaagcgctgc gtgcccacat cggcgtagtt gaaaagcaat
     1081 tcgcaccacg cgtccgcgcc gtcgcgtccg gcacgcccca cctcttggta atacgcttcc
     1141 agactgccgg gaatgtccca atgcgtcaca aagcggaggt cactgcggtc cacgcccatg
     1201 ccgaacgcgt tggtggccac caccaccggc gacggctccg agatgaaccg gttctgcgcg
     1261 gcggtgcgct cgccgtccgc catcgcgccg tggtaggtga tcggcgcaag gccctcgctt
     1321 tcaagacgtg cggccacgcg ttcggccatc ttgcgggtgg aacagtacac aatcccgcag
     1381 cggtgggctt ccaccatttg aacaagccgg gtaaacttgt gccggtgcgt cgcgcagcgc
     1441 gtcaccgcca gatgcaggtt ggggcgcgcg aacccggtca cccgcacttc cggggcctgc
     1501 cgcggcgcca cgcccaagcc gagttggcgc acgatgtcct cacgcacatc gggcgtggcc
     1561 gtggcggtca cggccagcac gcgcaccgcg cccaaccttt ccaccacatg tttgaggttg
     1621 aggtagtcgg gccggaaatc atgtccccac tggctgatgc agtgcgcctc gtccacagta
     1681 aacagcgcaa cctgcacagc ggcgaaggcc tcggtgaagc ggcggttgcg aaaccgttcc
     1741 ggcgccacat acagaagctt atagcgcccc tgacgcaacc cgtccagccg gtaggccatc
     1801 tctgcgctgt cgaccgatga gttgaggaag gtcgcgggga tccccttgcg atcgagcgca
     1861 tcgacctgat ccttcatcag cgcaatcagc ggcgagacga cgaccgtcac ccccggcaac
     1921 agcagggcgg ccaactgata gcagagcgat ttgcccgagc ccgtgggcat gaccagcacg
     1981 agatcgcggc cgtcgagaac ggtgcgcacc gcctcgccct gtcccgcgcg gaaggccgcg
     2041 tacccaaacc ggtcggacag ttcctgcttc agccgatcct ctgtccagag accgctcatg
     2101 ggtgtccacc ctctgcccgc aggtcaaacc tcgacacacc agccgtgccg atcgggcagc
     2161 accccgtact ggatcccctg caccgtgtcg tacagtttct gcagcacggg tccgcacccg
     2221 tcgggcgcgc ccacccggat cacccggtcg ccgcgtgtga tctcgtacac cggcgtgatc
     2281 accactgcgg tgccgcaggc ggcgatctcg gaaaactccg gcaactcatc atacggcacg
     2341 gcacgcgcct ccacactcat gccaaggtcc tttgcgatct gctgcaacgt gttgttggtg
     2401 acactcggca ggatcgaaca cgaatccggc gtcacatacg tgccgtccgg tttgatgccg
     2461 atgaaattgg aggtggcgaa ctcctcgacc agcgtgtggg tcttcgcatc cagatacagt
     2521 tccacggggt agccagcgtg tttcgccgac tcgtgggcgt agaggctggc ggcgtaattc
     2581 cccgcgacct tgacatcgcc catgccctgc ggcgcggcgc ggtcgtagtc atcgaggatc
     2641 agcgcgcgca ccggtttcag cccccccttg taataggggc cgaccggcat gaccatcacc
     2701 agaaacgtgt actcgtccgc cggggccacg ccgatctgcg cgcccgagcc gatcagcaga
     2761 ggccggatgt agagcgagcc gcccgtgccg aaaggaggca cgtaatcctc gttggccttc
     2821 accacgcgcc ggaccgcctc ggtgaacatc gccaccggca cctgcgccat acatgtgcgc
     2881 cgggcggtgt tggccatccg ctccgcattg gcctcgggcc ggaagatgcg cacgcggccg
     2941 tcctgacaat ggaaggcctt caacccttca aacgccgcct gaccgtagtg caggcaggag
     3001 gcagcgatgt gcatcttcag ccaggggtcg ctgaccagtt ccccttcatc ccacgctccc
     3061 tgccgccaga catagcggat gtggcaacat gtatccatgt atttgaaacc cagattgctc
     3121 caatcgatgt tctgcatgct tcgaaatcct tgttggggac gcgaccgccc tttgtgacgg
     3181 atgatttgag attgtcatct ttttcggcgc acgatgcaag cccctgtttt tcgtctttcg
     3241 caaagcgcgc ccgtcgggcg cgaattcccc ttttgaagtt tcggcggatt tcgtgtatgt
     3301 tatctcgttc tgtttttttg agaggtcgat tatgattcga gtcaaagtgg tcggcgcgac
     3361 cggatacggc ggcgtaggca tcacggaact ggtcttgcag catccgaagg cgaagctggt
     3421 ttccctgatc gcgcgggagg ctgtcgggca accgctctcc gccgtgtatc cgcatctggc
     3481 gggcttctgc gatctgccgg ttctggcggc agaggatccg cgtgcgcagg aacccgccga
     3541 cgtggtgttt ttcgctaccc cggacagagt gggcatgtgc ggtgctccgg aagagctggc
     3601 ccgcggtgcc aaggtcatcg attacagcgg ggacttccgg ttcacttccg cagaggcgta
     3661 cgccgattac gccacgcgga tcggcaaaga tccggtgcac gccgccccgg aactgctcgg
     3721 acgcaccgtt tacggtctgg ccgaactgca ccgcgacgcg atccgcacga gcacccttgt
     3781 ggggaacccc ggctgtttcg catgcagttg catcctcggc atggccccgg cggtccggga
     3841 gcgtctgctc atgccggaca ccatcatctg cgactgcaaa accggggtgt cgggcgcggg
     3901 caaaaaagtg aacgcggccc accattatcc cgcgcggtac gagcagatga atgcctacaa
     3961 actcgcgggg caccagcacg tgtgcgaagt cgagcgtgaa ctgggcctgc tcgcgggtca
     4021 ggaggtccgc atcaccttca ccgcgcaggt ggtgccggtc tgccgcggca tcctctccac
     4081 gctctacggg accctgacgc acgccatgcc ggaggccgaa ctcgtggcgt tgtaccggga
     4141 tttcttccgc gagagccctt ttgtccgcgt gttcgacagc cagaccgcaa tcggcacact
     4201 gaatgtgcgc ggcacgaact tctgcaacct tgttgtcagc gtcgatgcgc gtgtgaaccg
     4261 tctgcgtgtg gtcgcacaca tcgacaatct catgaaaggg caggccggtt ccgccctaca
     4321 gaacatgaac ctgatgttcg ggttacctga aaccacagga ctcgaccgac cggggatcta
     4381 tccatgagca ataaaaagtc cgccccaaaa aaggttgtgg ccgccgcagg ccggaaaacg
     4441 gcccctgccg cgtcccgcaa acaccccaaa ccgggtcttg gccgcggtct gggcgctctc
     4501 atcagccgcc cttccggcaa tgcggtccag accccgaacg tcgcggcgcg catggacgcg
     4561 ccggagcgga ccgggtatga cacagccgcg ccagacccgc gccggagcgt gcttgacgtg
     4621 ccggtcgcgg agatttcccg ctcgccgtgg cagccgcggc agacctttga cgccgagtcg
     4681 ctgagtgaac tggcggactc catccgggcg aacggcgtga tccagccgct catctgccgc
     4741 aaaaccggcg acgcccggta cgaactggtt gcgggcgagc gccgcctgcg cgccgccatc
     4801 gaggcgaaac tcgcaaccgt acccgtgatc ctcgtggagg ccgaagaccg ccgggccgcc
     4861 gagttggcca tcatcgagaa tatccagcgt caggatctga acgccatcga ggaggccgag
     4921 ggttaccaca cgctggccga aacgttcaac ctgacccagc aggaggtggc ggaccgcgtc
     4981 ggcaaggcgc gcgccagtgt ggccaatgcc ctccgcctgc tcgatctgcc cgacgaggtc
     5041 aagcagctca tcagcagcgg actgctctcc accggccacg ccaaggtgct gctcagcctg
     5101 acggacgtga ccgagcagac gctcctcggg cgcaaggcgg tgaccgaagg gctgaccgtg
     5161 cgcgcgctgg agaaggtggt tcagcgccgc aacaaaaggg atgcgggcgc gacactccgc
     5221 cgccccggcc agccggatct gcccgagagt tatgtgcgcg acctcaccga ccgcctccac
     5281 aaacacttcg gcaccgccat tcgtctctct tccagcgtga cgcacgcgaa cggcaagcac
     5341 accaagggca ttttggagat cgatttttac gacaacgatg atctggaccg gctgctgacc
     5401 ctgttcggca tcacgctgga ttgaaccgcg aggacgacgc gagggaaccg gaatgcggag
     5461 gatgatgctg ggatgggttt tgctggggct cacggggtgc gacctcggga caaccaaccc
     5521 ggagcccgta tccgcaccgc cgccgcaggt gtggacggcc gcgcgggcca gcaatgcctt
     5581 cgcgtatgtc gaagggctgg tgcgggactg caccccgcgc gatgcaggca cgccgggcgg
     5641 ggagaaagca gcctgctgga tccgcgaccg tctggccgaa caaggccttc cagcgcggat
     5701 cgaccgtttc accgagccca ccccgcgcgg accgcgcgaa ttccgtaacg tgctggccac
     5761 cctgcccggc accggggaca catggattat cctgctctcg cacttcgaca ccatgagcgg
     5821 catcggggaa gggtttcagg gtgccaatga cagcggctcc tccaccgggt tgctgctgga
     5881 actggcaggc gcgttgcgcg acgccggacc gcgcgaaccc tccatcctgt tcgggttcat
     5941 ggacggcgag gagtgtatga tcgcgtatgg cgcgcaggac ggctttcacg gcagcaaacg
     6001 gcttgcgcgg cagatgaaag aggaaaaacg gaagatccgg gccgtgatcc tcaccgacat
     6061 ggtcggcgac cgcgatctta aactcaccct gccgcgcaac agcaccgcag cgctccgtgt
     6121 gctggctttg gaggccgcgg aagccgtcgg cctccgcgat catatcggcc tgtttgacgg
     6181 caacatttac gatgaccacc aggcgtttct ggatttgggc tatccggcag tgaacttgat
     6241 cgacttctcc tacggaagcc gtccgggact caacgattat tggcatacgc ggcaggacac
     6301 gctggacaaa ctttcggtcg agagtctgcg gatcacgggc gcgatcgtgc tggaaatggt
     6361 caaccggctc tcgtctcccg cccgccgctg aagcgtccgc cggacagcgc gatcaggctc
     6421 gcggatgggc cttggcgtac gcatccttca gccggtcaat ggaaacgtgc gtgtagatct
     6481 gcgtggtgga gagactcgcg tggccgagca tctcctgcac actgcgcatg tcggcgccgg
     6541 cgttgagcag gtgggtggcg aaactgtggc gcaacttgtg cggcgtgagt tcctgcggca
     6601 ggcccgcagc ccggagccac accttcatct ggcgctcgat cgaacgcgaa gtcaacgcgc
     6661 ccccctcgcg gttgaggaag agcggcgcct gcggtcccgc tgtcgcgggc cagaggcgcg
     6721 tggccgcgtc gcgcagccgg atcagcgcat cacaggcggg ccgccccaag acgcacaacc
     6781 gttgtttgcg gcctttgccc tcgacaatcg tggtgcccga ctcgaaccgg atgtcgcgcc
     6841 agaccagcgc ggcaacctcg ccgatccggc accccgtgct gtagagcacc tcgaatatcg
     6901 cggtatcgcg taacgccgca tattcggccc acagcgaggg cgcccgcccc gcgtccgacc
     6961 gtttccgcag cgcggcaagc ggagccgcca gaagagcctc aacttcgcgc agactcagaa
     7021 caacgggcag gcgtttcccg agacgcggcc cgcgcagccc cgcgaacggc tgcgcggtgg
     7081 ttcgcccctc gcgctccaga taccggtaga aggtacgcaa cgaagagagc ttgcggcggg
     7141 tcgtggcggg cgcgtgcccc ttacggtgga aggcggagag aaaggcgcgg gcccgcgcac
     7201 ggtcgggttc gtcccatgcg aacggcggcc ccccgcgctt ctccgtcccc cacacaaatg
     7261 cggcatactg cccgatatcc tgaaggtacc ccgcgacggt atgcgccgag gcgttgcgtt
     7321 cggccgtcag ataccggaca aactgtccga ccgcagggtc acgcgccaca tcgatccggt
     7381 tcggcatcgg ctccggcggg gtgtcgccga accgcacctc cattgttgta tcggcattgg
     7441 cgtcgaattg cccatccatg acattctccc ggcgaatccg ggcccctccc ggaaacccgt
     7501 tccgcctacg cttcctttgt cccggaggtt tccgtcttgt cggatttgct cttcaacccc
     7561 agtttggccg cataacgctc aaaccccgga atctgcgcct tgtaacggaa caccatccgc
     7621 cgcatggcat accgctgccg cggcgaaagc gaacgcttgt ggttgaactg ttccgaaaga
     7681 ctgacgaaaa aggcctggtc atcgaaggac atcttccccc gctgcaccgg agcctgccac
     7741 tctgtgacgt gacccagcat ctccagcagc accggactct cgtggtcggt cgacggctcg
     7801 ctctcctccg ccgtcagccc gagcgaggcc ttcaccgtct cgaaatcgcg gatctgcgcc
     7861 gcgttctgca caacgatgcg gtccagcgcg gccatctgct tttcgctcag ctttcggccg
     7921 ctttccgcct gatgccgcag cgattccacg aatttcatct ggcgctcgga gagttccacc
     7981 tccttcagca ccgcaaaacg ccgcatcacc tcttccccgc tcggcgcggc gttgtcggtc
     8041 tccacgattt cgccgaaacc gcacgacttc aacaccgcct cgacctccgg gagttgctcg
     8101 cgatagcgca cggcgatttt ggcaagtgcc gtaagctgtt tttcggaaac cggtctcgcg
     8161 gatttctcaa tctgttcgcg caccgatttc acaaaccgtt cgtcgctgta cactcgtttg
     8221 ctgttccccc gctgcaccgc aggcgcccag accgtgaccc gctccagcaa cgacagcatt
     8281 gtctccacct tgccggcatc cgcgggcggt tctttggcct gttccatcca ctgctcgaac
     8341 cgcttgtaaa aatcatccag catcgcggtc cagccgatcc cgcccgcctc gatctcatca
     8401 aggcgttcct ccatgcgcgc ggtgaacccg acatcaaaca gcgaaccgag tttcccgacg
     8461 agcaggtcgt tgacctccag acccagcggc gtgggcatca actgccgctt ttcacgtgtc
     8521 gtatagtccc gcgagctcaa tgtctccaga atggaggcgt acgtcgaggg ccgtcccacg
     8581 ccgtttgcct ccagcgcccg gatcagcgac gcctcgctgt accgggcggg cggcttggtt
     8641 tccttgcgct cgctcagcca ttccagcggc accagcccgt cgcctttagc cagcggcggc
     8701 aaccgttgga cctcttcgac ctcctcttcg ggatcctcct cctcaggctt tttgcgccgg
     8761 atgtccagcg ccatcacctt caggaatcca tcgaaccgca cctccgaggc cgacgcggta
     8821 aagaggcagg cgcggcgcgg cgcgaccccg gttgacgcgg gcgggaccgg ttcgatctcc
     8881 acggttttct gcgcgatctt cgccgccgcc atctggctgg cgacgaaccg gcgccagatc
     8941 aggtcgtaca gcttgagaga ggaggcgtcc aagattcctt tgagactttc aggcgtccgg
     9001 gtgacctccg tgggccggat tgcctcatgc gcctcctggg cactcgcacg gctgcggtac
     9061 acattcggcg acgccggata atactccttg ccgtacgcgg ttatgatgtg ttcccgcgca
     9121 gccgcctgcg cgtcccggga gacgttgacc gaatccgtac gcatgtaggt gatcagaccg
     9181 acaggcccgc ccgcatcgag cgcgacgccc tcgtacagtt tctgcgccag gctcatggtg
     9241 cggctcggcg agaatccgca cacggtagag gccgcctgct ggagcgtgga ggtgatgaac
     9301 ggcggcaggg gcctgcgcgt cgtttcgcgg acccgcacct cccgcacccg cagcgtgcag
     9361 ccctcgagat cgctcaacag cgcgcgcgcc tcggtttccg aaccaatgac cggcttctcg
     9421 ccgtccactt tggcaagttt gaccgtgaac ggatccaacg gcgcaagggc gcgccggaca
     9481 cgtgcgccca tcacccagta ctcttccgga cggaaagcct gaatctgacg ctcgcgctct
     9541 gacaacagcc gcagggccac ggactgcacg cgcccggcgc tcagcccgcg cttgaggcga
     9601 cgccagagca gggggctgac catatacccg acgatccggt ccaacacgcg ccgcgcctgc
     9661 tgcgcgtcca cgcggcgcat atcgaggtgc cccggcttct cgaacgcggc gcggaccgca
     9721 cgcggcgtga tctcgttata catgacccgg tggaacggct tcccatcggc cacctccgcc
     9781 agcgtctcgc gcaagtgcca ggcgatcgcc tccccctcgc ggtccggatc aggcgcgagg
     9841 taaatctcgt cgcacccttt gaccgccttc tttaactccg cgatcacctt ggttttgcct
     9901 ttcgacaaaa catacttcgg cacgaagccc tgctcgatat ccacgcccag cgtacgctcc
     9961 ggcaaatccc ggatatgccc caccgaagac ttgaccacat agccggaacc cagcattttc
    10021 ccaatcgttt tcgccttcgc gggcgattcg acaatcacca gtttcttact catgtgcacc
    10081 tttcctcaca tcggttacgt cccaacccgt acggcccaaa tccgccgcac ccaaacatcc
    10141 cccccgggcc ggtcaacttg cccgggacac gcgggtcacg aagcctcccg gcaacagccg
    10201 gaccacccgc ttgatctgca accccaccag catcgcgttc acggtgcccg catccaatcc
    10261 gctgccccgc accaccgcat ccaccgaaac ctcctccatc ccgatctgcg ccacgacctt
    10321 ccgctcgtcc tcggagagga cgcacgccgg agggcgcgcc tcttccgtcc cgctccgcac
    10381 tgctgccggc gtctcgcgcg tcttcgcgcc ggccagcaga tcctccacct cctcgatcac
    10441 ctcgtccgcg gtcgtgacca gacgcgcacc ctcgcgcaac aggcggtggc aaccctgcga
    10501 caccggcgag tcgatccgcc cgggcaccgc catcaccgtg cggttctgat ccagcgcctg
    10561 ccccacggtg atcagcgtac cgctgttgag cggcgcctcg atcaccaaca cccctttgca
    10621 gagaccgctg acgatccggt tgcgcatcgg gaaggtttgg cggtccggct gcctgccgaa
    10681 cgggtattcg gagatcaccg caccgccctg cgccacaatc tcgcgcgcca acccggtatt
    10741 ctctttggga aagagacggt ccaacgcccc gcccagcacc gccactgtcc gccccttggc
    10801 cttcaccgcg cccgtatggg cctcggtgtc gatgccgaga gccaatccgc tcaccaccac
    10861 atatcctgcg gccgccagac ggtacccgaa gaaacgggcg gtttcgcgcc cgtataccgt
    10921 gggccggcgc gtcccgatca ccgccacggc aggctgattc aacgcctccg ccgcccccgc
    10981 gacatacagg accagcggcg ggtcggcgat ctgcttcagc agggccgggt attccggatc
    11041 cgcccatgtg accagccgaa cccccttttt ctccgcccgc gccagttccg gccccgccgc
    11101 cacccgccgc aattccgcgt gaaactgccg cgcccgctcg gcgccgatcc ccggcacact
    11161 caacaattcg gactcggacg ccccgaacac ggctgacaag tcaccgaacc gttccgccat
    11221 acgccgggtc gtcaccgcac ccaatcccga aatctgattc aaggcgatca acgcctcacg
    11281 ctctgtcacg tccgcctctg ttcattgctt atcaggatcc gccactgttg caagaaagtg
    11341 cggtaatagc atattccacc ctccctgaca agacaggttg cgtttggact tttccccgac
    11401 cggttccgaa acggcaattg acaaaaaaga aaacagcttt attttatggg tatccacgca
    11461 aaaagcagga atctgtacga ccatttcaag atcaatatcg tcggcagtaa gccgaacggg
    11521 acctgctgga ggtgatcggc acggcacccg tgccacgcat ctaaaaaagg agactggata
    11581 aatgaggagt tgtcttaaag cacggaagcc ggtggcgtgg tttgcaatgg ccgccttgtc
    11641 ggtgtatggc gggccgacgt cgattccgat acgccagggc ggcgtggaag ggcggccgtt
    11701 ctggaacaag gtgtcgccgt ggttcgagta tgcgccggcg ttcgatttcc ccgaggtcaa
    11761 cggggcggtg aagtaccgtt tcatcgcgaa acaccagagc ggtgcggaat tttcgttcga
    11821 ggcggagagc ccgcaggcgg cgttgtcgcc gatttgggag aagttgcccg ttggcgcaat
    11881 cgaactgcgg gtggcggctc ttgacgcaaa cggcggtttc atcgaaaccg ccccggggaa
    11941 tgcgaccgtt acaggcagtg cggttgcgat gaaaccgccg acagccgtgc gcaagttctg
    12001 gaaaaaggcg acgttccacg aggggatgta cgagtcggct gcgtgttcgt atgcacagtc
    12061 ggcccgcaag gccttcgagt tcgtgttgaa tgagccgtcg atccgctatc tccatgacca
    12121 cggaacgccc gatccgtcct attcgctcaa cggctatcca tcgaagatgc tcagtgcgga
    12181 gatcggcatt ctcctcgata ttgcaaagac gcgtccggaa caccgcgcgt tcgcgctcga
    12241 aaccgcgaag aaggcggcag accacctgat ccgcaaccgc tgcgccgctg gcacgccgct
    12301 tgaatacttc acgaagacct atgaaggcga aggggagtac ggccggtcga agcagaatgc
    12361 gggactcaac atgctcatct atcccgccaa agccggcgag gcgcttgtga agttgcatgt
    12421 ggcgttggca agggacgaag ggtatctgga tgcggcggag aagatcgcgg cgacgtactt
    12481 gaaacttcag ggaacggacg gcacgtggcc gctgaaggtg cgcgaggata ccggcgagcc
    12541 ggtggcgccg aaccgtctta tcccgttgcc ggtgatcgac ttccttgaat cgctctatgc
    12601 cgttacgaaa aagcccgaat accgtgcagc ggcgaaccgt gcgttcgcgt atgtcgagaa
    12661 gggtcccttg aagacctgga attgggaggg gcagttcgag gacacgaaac cgcgtccgcc
    12721 ttatctcaat cccacgatgc acgaggcggc ttcgacggcg atctatctgc tgaagcgctt
    12781 tcccggggac gcgaagcggc tcgaactggc gatggaactg gtccgctggg ccgaagacca
    12841 gtttgtcgcg tgggagaaac ccgaggtcgg cgacagaaga gcgcatcact tcaatcccgc
    12901 gaactggtac gtgccgggca cgatcgagca gctggcaatg ggcgagccga tcgacgcttc
    12961 gtccgcaaag atgatcgaag tctatctcgc gctccacaag gcgaccggcg ggcacgaata
    13021 ctgggccaag gcgcgggtgc tcggcgacac gatcacgcgt gcgcagtggg aagacggatg
    13081 ctacaagacc ttctgggtga aggcggaccg cgacgaccag cggtaccatg tctggatgaa
    13141 ctgccatatc tacacggcga gggtgatgaa cctgctggcg accgtggaac attggctgaa
    13201 cggtaagcct aaaaggagca gttgaacggg tagaattcta gcaaagcccc gtaaacatcg
    13261 ggttttgacg tattgagatg cgggcggagc ccgcaatctc aaaggctgta agactgtgag
    13321 gcggtaagac tgtgaggcgg ttgtgtcccg tggtgaagca atcgatcccg atagcgatag
    13381 cgatcccgat accgatagcg atcccgatac cgatttggat gaaaccctga aaccacgaaa
    13441 ccctgaaacc acgaaaccct gaaaccacga aaccaaagag agttgaaggt tcaactgctc
    13501 tttttagggt aagtgaaaag ggtcggtatt gacaagaccg cccggggttt gtgataattc
    13561 tcaccgtaaa ccttgttttg aactcaatgc cgcacacgcg gcgtatctag ggggtcgttt
    13621 atgtccgatc atatgccgat ggccacgcgc gtcaaactca gcgccatgat gttcctgcag
    13681 ttcatgatgt tgccggtctg gttcatccca atgttcccat atatcaccga aatggcaccc
    13741 aaaggcagtg ccctgccgtt gctctgcggc atgatcatgg gcttcggtgc cctggcctcg
    13801 ccgatcttcg gcatgttcgc agaccgactg atgaacagcg agaaggtgct ggcgctctgt
    13861 aacttcgtga cggctgtgct gctggcctac gcctacacga tcaagactcc ggggatgttg
    13921 ttcgtgacgt tgctgctggt gatggtgttt tacatgccga cctggtcgct gaccgccacg
    13981 atcgcaatgg caaacagcac aaccgaagcc ttcccgcaga tccgcgtgtt cggtacgctg
    14041 ggctgggtcg cggcagcgat tttcagcatc atcggcacca aggttttcgg catcgcccag
    14101 tttgacagca ccccgtacat ctttttgggc ggggcgatcg ctgcggcact aggcggcgtg
    14161 ctggcctttt tcctgccgcc gacccaaccg ccggccaagg ggcagaagat gtcgctggtc
    14221 gatacgctgg ggctgcgcgc gctggtattg ctcaagcgcc ccgacttttt ggtgttctcc
    14281 gtgctcatcc ttctggcgat ggtgccgttc caatggtaca acgtctacaa cagcacctat
    14341 ctgaaggagc gcgggtacga atatctgacg ggggtcatga accttggaca ggtgctggaa
    14401 ctggtcttca tgctcctgat cccggttatt ctgaagaagg cgggctataa atgggctatg
    14461 gttttgggat tgctggcgct ggtcttccgt tacggcatgt tctatctggg cgcggcgcac
    14521 ggactgcaga tctgtgacta tgccggcatt ctgatccacg ggctgatctt cggcatgctc
    14581 gtcgtcggca gccagatgta tgtggatgcg gcggcacctg ccgagttgcg cggtcaagcg
    14641 cagggcttca tcgggctgat catgttcagc ttgggaacct tcctttcaaa cttcgtgttt
    14701 gacgggattc tcaagaagaa cgtggccgca gacggcaccc atgtgtggac gaccccgtat
    14761 ctgatcgcaa tggtctccgc gctggtgttg gcggtgctga tggccgtggc cttcaagcct
    14821 gccgttcagg agaagcggca gggcgcgtga gccccctgac cgcctaggcg ggaaccctgc
    14881 ggaacgcgcc gtcacacgcc tgtcgaaccg aagccggccg ttccgcgcac ggtcccgccg
    14941 agttccgcga cttcccgcac ctcggcgcgc gccaccggcg cgatcaccat ctgcgcgatc
    15001 cgcatccctg cggtaatttc aaacggcgtg tcgccgtggt tgatcaggat cacgccgatc
    15061 tccccgcggt acccctcgtc caccgtgccg ggcgtgttca gcagcgtcac cccgtgccgg
    15121 agcgccagac cgctgcgcgg gcgcatctgt gcctcggttc ccggcggcag cgccaccaca
    15181 agaccggtgc ggatccgttt gcgctccccg ggttgaagca cggccgactc gcaggcactg
    15241 acatccatgc ccgcatcacc gggatgcgca tacgacggca ccacggcttc gggatggatt
    15301 ctcatgaatt ccactgtcat gcccacagca taacagagcc gacgccggta tgacaacccg
    15361 tggtcgtcag tgctgtgctg aaacgtcccg tgaacggggc gcacagaacc gccctcggcc
    15421 ctgtgcgcgg tgtcggttct gtgcgcggtg tttacaccgc cagccagcca gcggccagcg
    15481 ttgcgctggc cgccatatgt cttgtgccct tatcccgccg tgatcgcgat ccgtcgcggt
    15541 ttgacctcct cgcgtttggg cagcgtcagc accagcatgc cgtttttgca ggatgcctgc
    15601 accttcgccg tgtccacgcg ctcgggaagc tcaaacgccg cgcgataccg cgcctcctga
    15661 atctcattca ggatcaaggt gtagtccttg aaggaagcca cctctttttc ggccgtgacc
    15721 gtcagcgtac ggtcctccaa agtcaactca acctgttttt ccgacacgcc gggcaactcc
    15781 atcgtgagcg tgaacgcctc ttcgctttcg gtgatccggc tcacaggggt cagctccaaa
    15841 cggttggcct tctcggcttc aactctatca cgtttcatca agctcatgct ctcctccttt
    15901 cctttcgcga cccggtcaca gtttctcaat ggcgatcttg cgcttctccg ccgccgcctt
    15961 ccggggaatg gtgacggtca gcacaccgtt tttcacggtc gccttgatgc ctttctcatc
    16021 cagttcgaag ggcaggttga aactgcgctg gaactcattc tccgtgccgt ccggcaactc
    16081 gcgcttgcct ttaagcgtca gcacgttcgc gtcaaccgcc acgtccagcg tatccggctc
    16141 catgccggga acctccgctt cgaccaccag acctttttcg ctctcccaca cgttcacacg
    16201 cgggttggcg ccaacacggg cacgccaagc cgaattgaag aggtcataag ggaaatcacc
    16261 catcaactcg tcaaagacac gccagggatt gccccaagga ctcagatatg ctctcatggt
    16321 atttctcctt tcctttaaca ctctattttt cggaaccaac ggaaccgcct atctcttcgc
    16381 attgtccatg ccaacttttt cataccgatc tttgtaatac gtaagacatt atcacactat
    16441 gagttatata aaaaaacaat gccgtgacac acggccctgt ttgggtcaac atgactcttt
    16501 ttgccgaccg catgacatta tgtcacagtt ttaaggccca aaaatgcggg ccgcttcttc
    16561 cgggcctttt gaccaaagtc ttttctacca cattttgtgg ttgaattcgt tgccggatca
    16621 atatatagtg cggcctgtta ttcgtttgat tcttttaagt cctcttttta tagaggaatc
    16681 cgcacgtccg atagagacgg gtgcaggggg gaagggtttt tatgagcagg tatgagcagc
    16741 cagacttgat tctccggatt cgtaaacgtg acggtcgttc ggaggtcttc gattcgaata
    16801 aaattgtggg cgccattctt cgggccggag aagcgacagg cgagttcgcc ggggacgagg
    16861 cgcgcaaact gaccctcaag gtcatgtcga tcatccactc cctcaccgac acgcttgacc
    16921 tctctgtcga aaacgtgcag gacatcgttg aagaggtgtt gcttgcctcg ccctaccgcc
    16981 gcacggccaa ggcctacatc atttaccgcg accagcacgc ccggatccgc gaaatgctcc
    17041 gcaaagcgga cgtggacctg atcgaccggt atctgggcaa agcggactgg cgtgtgcgcg
    17101 agaactccaa catggcctac tcgctgcagg ggctcaacaa ctacatctca agcgaggtca
    17161 gcaaagccta ctggctcaac aaaatctatc cggcggagat ccgtgaggcg cacgacaacg
    17221 gcgcgttgca tctccacgat ctgaatctgc tgagcgtcta ctgcgtggga tgggatttgc
    17281 aggatttgct gcgcagcggc ttcaagggcg tggccggcaa agccgaaagc ctgcccgcca
    17341 aacatttccg taccgcgttg gggcagatcg tcaacttctt ctacacgctg cagggcgagg
    17401 ccgccggcgc ccaggccttc tccagcttcg ataccctgct cgcccccttc gtgcggtatg
    17461 accggctcaa cgccgccgag gtccggcaag ccttgcagga gttcatcttc aatgtcaacg
    17521 tgccgacgcg cgtcggcttc cagacgccct tcaccaactt gacgatggac ctgatcgcgc
    17581 cgtcgacgct ggcgaacgag catgcggtca tcggcggcaa ggagatgccg gagacctatg
    17641 ccgatttcca gcacgagatg gacatgatca acaccgcgtt tctggaggtc atgaccgagg
    17701 gcgacgccaa ggggcgcgtc ttcactttcc cgatcccgac ctataacatc accgaggatt
    17761 tcgactggga acgtcccggt ctggagaagc tctgggaagt cacggccaag tacggcatcc
    17821 cgtacttcgc gaacttcgtc aactccgaca tgagcccgga cgacgcccgc tcgatgtgtt
    17881 gccgtctgcg cctcgacacg cgcgagttga accggcgtgg cggcggcctg ttcggtgcca
    17941 atccgcagac cgggtcaatc ggcgtggtta cgatcaatat gccgcggctc ggttacctcg
    18001 ccaccgatga ggacgatttc ctcgcccgcc tggacaacct tatggggatc gcgcgcaaca
    18061 gtctggaaat caagcgcaag gtgctggaac agttcaccgc gcagaacctc tacccgtaca
    18121 cgcgtttcta tctgcgtcag gtttaccagc gtttcggcac ctattggcag aaccacttct
    18181 ccaccatcgg actcgtgggc ctgaacgagg cgtgcctcaa cctcttcggc gaggatatcg
    18241 gtagcgggcg cggtcgcgcc tttgccttga ccgtgatgga tttcatgcgc gcgcggctgg
    18301 ccgactatca gg
//
LOCUS       DAOSRB010000036        18302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3197_length_18302_cov_7.754261, whole genome shotgun sequence.
ACCESSION   DAOSRB010000036 DAOSRB010000000
VERSION     DAOSRB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18302
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3197_length_18302_cov_7.754261"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1109)
                     /locus_tag="PLZ74_03425"
     CDS             complement(273..1109)
                     /locus_tag="PLZ74_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOR97405.1"
                     /translation="MTIIEPHIHMLSRTTDEYQAMYRAGIRACVEPSFWLGTNRRYAG
                     SFFDYFQLILEFETIRAARFGIDHYCAIAMNPKEADDRGLADEVIDGLVPYIEHPRCV
                     AMGEIGLNLNTPDEEYAMIRQLRMAEERKMPVILHTPHVDKARGTRRILEIIRAEGFT
                     VERIDVDHNTEETIRMTLDSGCYAGMTVYPYSKLTPQRVSDMVKQYGSDRVIVNGSAD
                     WGVSDPLSLIKVVEHMRGDGHSEETIRDLVFNTAMRFYSASPNWKPDFGIEPQDPRTY
                     QR"
     gene            complement(1168..2115)
                     /locus_tag="PLZ74_03430"
     CDS             complement(1168..2115)
                     /locus_tag="PLZ74_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOR97406.1"
                     /translation="MSKKKKRYAARLPQQVRGGIRAQSAGPQGGSTRVWWSRRWLAFL
                     ENYRLGARLGRGRSYAVAGQVSALRVEAGEVTAAVQGADKAPYRAEIRFAVLSEAAKA
                     RVTAALRQQPMLLARLLIGDLPERIEGLLQEEGCPLFPQREQDLSSRCTCPDYVNPCK
                     HLAAVYCLLGEAVTTDPLLLLALRGLDRVTLFGPETPGVRTPAEKAPHMESVPETGCG
                     AAEFYGTPQAAFEDFGPAPRAVLPAPLISRLGPLPFWRGQERFVDTLEPLYARAASRG
                     WTVWSGEPLDLRREEEKVILRGATLHLKQRRMRADPLGF"
     gene            complement(2112..5153)
                     /locus_tag="PLZ74_03435"
     CDS             complement(2112..5153)
                     /locus_tag="PLZ74_03435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR97407.1"
                     /translation="MTILHSCWHASHLHIWGEVEACASGLPDGPRSAVAVSPFDAGAE
                     RLCHLLRPLMPGTGETPQPATLELFLPSRAWGSGFRPVPSQPFFNNAVFDGAGEEDAP
                     DFPHPWRVTAVVLPWRATLVLLGLVREPRLADELFIGESLLGFATLFRFAGALVARGR
                     FLPALHRTSEKVYEARWIPALDAADRKRLERLAPRLPPAAFAAGNGSPREALEELVDR
                     LVRASVVTTLSRAHAERGPWYSPHDAWFAALRGETRQVRWKTQAELEALAGALRQWRQ
                     PVESGEPGKVPRFTLEVPDGPEGAWFLRVLAEEGPEGEVPPPAWDAAFLLSLGQASLL
                     FPPLARAETRGGKFGCALSEEEAHAFLTVAAAVLEAAGYAVNLPPQWRPGHASALALE
                     ADLTPLTGEDPTTGTLSEARVAVTWSVTFNGTPLTKEEAVQLERAETPLVSLRGQWQT
                     LDLKRVREALRAQQRTDTVPVRQAVRLALGTEPGAGGLEIARARGRGWLDPLLDGLAG
                     GMSGMESLPVPTGFRGVLRPYQLRGFRWLAFLRAWGFGACLADDMGLGKTVQALAFLL
                     HEKDRGERRPVLLAGPMTVLGSWLNEARRFAPELRLLLHHGPGRAKGEDLARAAMDAD
                     VVVTSYALLHRDYAALRRIGWSGIVLDEAQNIKNPDTRQARAARALQADYRIALTGTP
                     MENHVGELWSVMDFLNPGLLGRRAVFRETFFRPIQAGTDPGARARLRRVTAPFILRRL
                     KTDRRVIADLPAKIEAKVFCPLTREQAELYEEVLESFRRDAEASEGIERRGLILAVLT
                     RLKQVCNHPAHFLGQSENLARRSGKLERLVERLEEVFARGESALVFTQYAVMGKLLAH
                     HLCRTFARETPFLHGGLTRPERDRMIRAFQNGTRPQAFVLSLKAGGTGLNLTRANHVF
                     HFDRWWNPAVENQATDRVFRIGQAREVMVHTLLCNGTLEERIDALIADKTGLANEVVV
                     SGEEFLTGLPDDALLEVLKLDAEVVDEEDAAPEQGGWGGWA"
     gene            complement(5245..8367)
                     /gene="priA"
                     /locus_tag="PLZ74_03440"
     CDS             complement(5245..8367)
                     /gene="priA"
                     /locus_tag="PLZ74_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOR97408.1"
                     /translation="MATEISADRLFDYAVPDALAPRLRPGQRVRVPFGKRTLDAYVVE
                     IAEDTALEPGPPELFSLSGGSDKTDGIRTRRLRPILAIEDDVPFLSPTLIALARWMAD
                     YTCSPFEAALRCMVPAAVRNRDIKVKERFYVTPCPGEFALTSRQNELLENLRRVDGGW
                     LHSLTREFACAPQTLRTLAAKGAAVIERRAVRRDPLANRKVLPTRPLALMPEQASALK
                     LIIAAVDAGVRPESGARASPPAHAGASQTARRPADVSPAGGDARAPDLTPDLTKDGTN
                     ATSLKFPGMIHSEPGTEAHWHSRGYVPHFDYPGLVQFITFRLVDSLPSSVIDAWKSEL
                     GIVNIVPTPPSANEELIRRIARYEDAGHGACYLKQPEIAREVSEVLRHGDGTKYRLLA
                     WSIMPNHVHALIEVEQADSVAQIVHDWKSVSAHRINRCLGRKGALWQPEYFDRYVRDP
                     NHFHSVVAYIQANRGIESGERTDLCAMQGEGAESGARASPPAHAGASQTAGRPADVSP
                     AGGDARAPDSAHAPKPVLLFGVTGSGKTEVYLQAIAHALQRGLGAIVLVPEIALTPQT
                     VQRFASRFGKRIAVLHSALGDGERFDEWHRIRNGEATVVVGPRSAIFAPVRNLGLIVV
                     DEEHEPSYKQDEVPRYNARDVAVMRGWMEHCAVVLGSATPAMESWLNVEKGKYVAAHL
                     TQRVAGRPMPRVQVVDMRLETARSGHAQLFSEPLLEAIRLRLERGEQTILFLNRRGYA
                     NALLCPRCGYAAACDACSVTFTYHRADACLRCHICGGWKHVPDVCPLCGDPAFRYAGF
                     GTQRAETALAACFPQAKILRMDADVTSRRRSHDEILAQFKSGRADILIGTQMIAKGLD
                     FPNVTLVGVLAADMSLHMPDFRAAERTYQLLAQVSGRAGRAELPGEVLIQTYSPGHPA
                     ICAAASDAGFEPFAARELAEREAGGYPPYARLICLTFRGASEEKVRFSAEAFARALSK
                     TTGNAAAVRISGVCAAPLAKAKGLYRYQLLLRAPTTRQITRPLREVMRRPLLASGVTL
                     AVDVDAINLM"
     gene            complement(8461..9222)
                     /locus_tag="PLZ74_03445"
     CDS             complement(8461..9222)
                     /locus_tag="PLZ74_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOR97409.1"
                     /translation="MTRHQKDGFTLVELLVVIGILGILSAALFPAIGNAVMAANMAAV
                     GTRGKDIFVAITSANMEREPLGLGNVWPDEMSKATGTLSQDDQDRQTASTDSTKYFAY
                     LYDEENAGTTDWSPVAEGFDYSKLAGAGIPSCGKRNLSEEYNMWTIACNLRDEMEDVI
                     PILVTRNIDASSLAAKYNDNKANTKLNFSSSDPKPFSNKGFVMVRKGGAILKGRGKYR
                     EMRVIYNNQQFDTTLQQDAQQLKYLTPKTSVTPGG"
     gene            complement(9386..9901)
                     /locus_tag="PLZ74_03450"
     CDS             complement(9386..9901)
                     /locus_tag="PLZ74_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97410.1"
                     /translation="MKRSIRVGLIALVWVAGWVQAQPPPRAGRPRPPRTINRSNWVGK
                     HVLTPEFMEQVGLRGAQAEQLKAELEEIQAQAQRLEETINREALRQGELARNVLSEPG
                     ASVDEIMAIIERIGRHRTEQAKLATRQLVVIRDRLTAEQRKKARELIAAEARRRMAQR
                     AAERAARERAP"
     gene            complement(10088..12589)
                     /gene="mutS"
                     /locus_tag="PLZ74_03455"
     CDS             complement(10088..12589)
                     /gene="mutS"
                     /locus_tag="PLZ74_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOR97411.1"
                     /translation="MAGDKLTPMMRQYRRIKAELPQDVLLLFRLGDFYEMFFEDAREA
                     APILGVALTRRTGIPMCGVPHHALDAYLAKLIRAGRKAAICDQMEDPAQAKGIVRREV
                     TRVVTPGTVTEDEILDAARNNYLAAVYAGKGLGLALLDLSTGALTVEEAPDAAVLAEQ
                     LNRAAPSECLVGRDQRNDPRLAALLAPDAPGMVTEIDDWLLEYDCAYDRLLRHFGVHS
                     LEGYGCDGRAAQVCPVGALLHYVHEELRHPVGHIRTLRVRRAHEFLTLDENTCLNLDL
                     IPPRGQSPQATLLGVLDHTRTPMGARMLRAWLTRPLRQAEAIRCRLDAVDRLTKQRTL
                     LNELRESLGTVRDLERLIARLGAGHGNARDLNALALSLHPVPGLRERVAAAGDPLLAE
                     LAERLHALPELVGLITAAIAETPAAGLKDGGIIRAGYHAELDELRALATEGHAWLAAY
                     QASEQERTGIKTLKVRHNKVFGFYIEVSKGQAGAVPPEYQRRQTLVNAERFITPELKT
                     YEQKIFGAQDRATALEFELFCGIREQAVEQTAAIQETADALAHLDVLAVFADRALALG
                     YVRPEITEDDTLEIRDGRHPVVEQLPDAGPFVPNDTRLDGHRNQIMLITGPNMAGKST
                     YIRQVALIAILAHTGSFVPAREARIGLLDRVFTRVGAGDDLARGRSTFMVEMQEAANI
                     LNNATKRSLIVLDEIGRGTSTFDGISIAWAVAEYLHNTPRVKAKTLFATHYHELTDLA
                     LTLSGVKNYTVQVREHGDTVVFLRRIAPGTADKSYGIHVARLAGMPDEVIDRSREILA
                     NLEEGELESGLPKLARRSRRRNDDDIPGQLTLFDL"
     gene            12763..14115
                     /locus_tag="PLZ74_03460"
     CDS             12763..14115
                     /locus_tag="PLZ74_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HOR97412.1"
                     /translation="MIASPVYLASVVALAVAVLLILIIRLKQQPFVSLLLVSMGTALA
                     AGMPPEKVIACIEKGMGGTLGFIAVVVGLGAMFGQLLEVSGGAERLTLTLLRVFGKER
                     ATWAMALAGFLVAIPVFFDVGFIILVPLVYTLSRETKKSLLYYGIPLLAGLAVTHAFV
                     PPTPGPIAVAQLVGADLGRVIFYGVLIGLPCAIVAGPVFGTWIDRRIHAAVPAYMETE
                     AAKKQWDEKALPGFGRVFGILLLPLFLILLNTVTQAVLAEGEYATVRHVFAFIGHPFF
                     ALLAATLLAFTFLGTCRGLTREKVNEVATSALAPAGLIILVTGAGGVFKQVLVDSGAG
                     KALAGTLAHTGLHPVWTAFLIALLVRAVAGSATVAMLTAGGIMAPLLAGQRVEPALIV
                     IAIASGATALSHVNDSGFWLVNRYFGLSVKETLRSWTVMETLIGLIGATLAWLFSYVA
                     "
     gene            complement(14132..15148)
                     /locus_tag="PLZ74_03465"
     CDS             complement(14132..15148)
                     /locus_tag="PLZ74_03465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOR97413.1"
                     /translation="MKQYSVGLVGIGAVGTEMIKVLRQRKFPAREIRIMATRERDEEV
                     AGETFHVIEARPEAFDGLDIVLFAGTEGSKGASKQFGWEAVRRGCVVIDNGDDFRMDA
                     RVPLVIPEINGEALTAHQGFIANPNCSTIITLMGIAPLHRRVPLRHMTAVTYQSVSGT
                     GRAAVEELEQQVRAYAAGNEPQVCAYPHPIAFNVLPQIGGAKPEMPGFTSEEAKLTFE
                     ARKILGCPDLRIAATCARVPVFYAHSVAVHAEFSAPLAPEEARAILANAAGIRVIDDL
                     AAAQYPMPLNTGGGDTVNVGRIRADPSVENGLALWCCGDNIRKGAAQNAVQIAEELIK
                     RGLL"
     gene            complement(15170..16447)
                     /locus_tag="PLZ74_03470"
     CDS             complement(15170..16447)
                     /locus_tag="PLZ74_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR97414.1"
                     /translation="MKRQWIGWVMAGILAAGIAADAPAGEEGFVTLFDGKTLNGWIQR
                     GGKSPYRVEDGAIVGCFGDAGRNSFLCTEKEYGDFILRLEFKFEGGNSGVQFRSAARK
                     HKDGSDSVYGYQAEIAGRSPVTARIYDEGRRGFKFKRTWLDNTPAEVTAKSVAAYKQG
                     DWNRMEIQCVGPSIRTWLNDVPVVNYFDDAALAGFIGLQVHAIKNPDGKPCARWRNIR
                     IRDLGTSRWTPFFVKGEDGRYALRGARFVLPEDWSFNHEEGILIGQHKEGEKRDGLVV
                     SDRNYSDFIVRVTYQLFGGNSALYFRAEEVNTPWLLKGFQNEIAGNAKDSALWHTAGD
                     KTKGRGWVAQNDEFIAQVRNPKGWNTTCTVAYGDRILSILNGQRTVDILDPLCEKSGK
                     VALQLHGGANGKMWFKDFEILEITPEMRALIDR"
     gene            complement(16555..18222)
                     /locus_tag="PLZ74_03475"
     CDS             complement(16555..18222)
                     /locus_tag="PLZ74_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR97415.1"
                     /translation="MTLRKIFEDTASRRPAAIALRYHRDQAWQSRTYESLMLGVRQMA
                     EAFGRLGLKPITERVAMIMENGPEWIETYLATAGAGVAVVPIDPKLRAAEVSYILNDS
                     EAVAVLTDTRHLDMLEVILTDLPQVRFIVVTDGAGERLAPIAGRPCYDYETLRAGVAN
                     EPLLWYADHRPDPQDVASIIYTSGTTGTPKGAMLTHLNFCSDAFGCFDAIGDVITAED
                     DFLVVLPLFHAFSFTANFVVPLVRGCGMSFIASLRSIGEDIRTLRPSILIAVPLLVEK
                     MFNKIDDKLRRNRIARLLFRLGLGCWVGKKVLKGLGGRLRFLIVGGAPCPLYVLNGFR
                     RLGVPIVEGYGLTECSPVVSITNSKNSRVGTIGKKLPNIEVRIADPDPQGVGELQIRG
                     PIVMKGYFKNLEATREAFDGEWLRTGDLAVQDAEGYLTICGRKKALIVNREGKNIYPE
                     EVENVLSRDPLIADVVVIGYTVGGIPGERVGTIVVPDLEAVAAAHEGVEPPWETVERL
                     IRERVRTQCECLTEYKHPRKIKVRREPLERTSIQKVRRCVYQGQLNE"
BASE COUNT         3207 a         6074 c         5633 g         3388 t
ORIGIN      
        1 gatcgtcgcg ggtcgacgat cccgatcccg atagcgatag cgatcccgat cccgaccccg
       61 atttggatga aacccagaaa cccagaaacc caagaaagtg tcccgtggtg aaacaatcga
      121 tcccgatccc gataccgacc ccgaccccga tttggatgaa accctgaaac ccagaaacct
      181 tgaaaccaga aaccctgaaa cccagaaacc agaaaccaga aaccctgaaa ccctgaaacc
      241 aaagagcgtt gctttcaacg ctctttttag gtttaccgct gataagtacg gggatcctgc
      301 ggttcaatgc cgaaatcggg cttccagttc ggcgaggcgc tgtaaaagcg catggcggtg
      361 ttgaaaacca gatcccggat ggtctcctcg ctatggccgt ccccgcgcat gtgttccacc
      421 accttgatga gcgaaagcgg gtcggagacc ccccagtcgg ccgagccgtt gacgatcacg
      481 cggtcggagc cgtactgttt caccatatcg gagacgcgtt gcggcgtgag tttagagtag
      541 ggatagacgg tcatgccggc gtaacatccg gaatccagcg tcatccggat ggtctcctcg
      601 gtgttgtggt ccacatcaat gcgttcgacc gtaaagccct cggcccggat gatctccaga
      661 atgcgccgcg tgccgcgcgc tttgtccaca tgcggcgtgt gcaggatgac cggcatctta
      721 cgctcctcgg ccatacggag ctgacggatc atcgcatact cctcgtcggg ggtgttgagg
      781 ttgaggccga tctcgcccat ggccacgcag cgcggatgtt cgatatacgg caccagcccg
      841 tcgatcacct cgtcggccag cccgcggtcg tccgcctcct tggggttcat cgcaatcgcg
      901 cagtaatggt cgatgccgaa acgcgccgcc cgtatggtct cgaactccag aatcagctgg
      961 aaatagtcga agaacgatcc ggcgtacctc cggttggtcc cgagccagaa agagggctcg
     1021 acacaggcgc ggatccccgc gcggtacatg gcctgatact catccgtcgt gcgcgacaac
     1081 atgtggatat gcggttcaat gatcgtcatc ttgttcccct tgttcatcct atgaaacgtt
     1141 ccgtcctccg ttcgggttca ccggcggtca gaatcccagt ggatcggccc gcattctgcg
     1201 ctgtttgagg tgcagggtcg cgccgcggag gatgaccttc tcttcctcgc gccgcagatc
     1261 gagcggctcg ccggaccaga cggtccaccc gcgtgaggcc gcgcgtgcgt acagaggctc
     1321 cagcgtgtcg acgaagcgct cctgcccgcg ccagaagggc agcggcccca accggctgat
     1381 caggggtgcg ggcaacacgg cgcgcggtgc gggcccgaaa tcctcgaacg ccgcctgtgg
     1441 cgtgccgtaa aactccgccg cgccgcatcc cgtctccggc acactctcca tatgcggcgc
     1501 cttctccgcc ggagtccgga cgccgggcgt ctccggtccg aacagggtca cccggtcgag
     1561 gccgcgcaat gccaacagca gtagcggatc cgtagtgacg gcctcgccca gcagacagta
     1621 gaccgcagcg agatgtttgc acgggttgac gtaatccggg caggtgcagc ggctcgacag
     1681 atcctgttcg cgttgcggga agagcgggca cccctcttcc tgcaacaggc cctcgatccg
     1741 ctcgggcagg tcgccgatca gcaaccgcgc cagaagcatc ggctgttgcc gcaacgccgc
     1801 cgtgacccgc gccttggctg cttccgacag gacagcgaaa cggatctccg cgcgataggg
     1861 cgccttgtcc gccccctgta ctgcggcggt cacctcgccc gcctcgacac gcagcgcgga
     1921 cacctgtccc gcaaccgcat aactgcggcc gcgaccgagc cgtgcgccaa gccgatagtt
     1981 ctctaaaaag gccagccagc ggcgactcca ccagacgcgt gtcgagccgc cctgcggtcc
     2041 ggcgctctgc gcacggatcc cgccccggac ctgctgcgga agccgcgcag cataccgttt
     2101 cttctttttg ctcatgccca gcctccccat ccgccttgct ccggcgcggc atcctcctcg
     2161 tccaccacct ccgcatcgag tttgagcacc tcgagcaggg cgtcgtccgg cagcccggtg
     2221 agaaactcct cgccgctgac caccacctcg ttggcgagcc ccgtcttgtc tgcgatcagc
     2281 gcgtcgatgc gttcctccag tgtgccgttg cagagcaggg tgtgcaccat gacttcgcgg
     2341 gcctgcccga tccggaacac gcggtctgtg gcctggttct ctacggccgg attccaccaa
     2401 cggtcgaaat ggaacacatg gttggcgcgc gtcagattca accctgtgcc gcccgccttg
     2461 agcgagagca cgaaagcctg cggccgcgtc ccgttctgga acgcccgtat catccggtcg
     2521 cgttccggcc gtgtcaagcc gccatgcagg aacggcgttt cgcgcgcgaa ggtccggcat
     2581 aggtggtgcg ccagcaattt acccatcacg gcgtactggg tgaagaccag cgcgctctcg
     2641 ccacgcgcga aaacctcctc gagccgttcg accaaccgtt ccagtttgcc ggaacgccgc
     2701 gccagattct ccgattgacc gagaaagtgc gcgggatggt tgcacacctg tttgagccgt
     2761 gtcagcaccg caaggattag accacgccgc tcgatcccct cgctcgcctc ggcatcgcgc
     2821 cgaaaggatt ccagtacctc ttcgtacagc tcggcctgtt cgcgtgtgag cggacagaag
     2881 actttggctt cgatcttggc gggcaggtcg gcgatcaccc ggcggtcggt cttcaggcgg
     2941 cgcaggatga agggggctgt gacgcgtcgc agccgagcgc gcgccccggg atcggtcccg
     3001 gcttggatcg gccggaaaaa cgtttcgcga aacacggcgc gccgacccag taggccgggg
     3061 ttcagaaaat ccatcacgga ccacagttcg ccgacatggt tctccatggg cgtacccgtg
     3121 agcgcgatcc ggtaatcggc ctgcagcgcg cgcgccgcgc gcgcctgccg tgtgtcgggg
     3181 ttcttgatgt tctgcgcctc gtccagcacg atcccggacc agccgatccg ccgcagtgcg
     3241 gcgtaatcgc ggtgcagcag cgcgtaactg gtcaccacca catccgcgtc catcgccgcg
     3301 cgcgccagat cctcgccttt ggcccggccg ggcccgtggt gcagcagcag gcgcagctcc
     3361 ggcgcaaacc gccgcgcttc gttcagccag ctgccgagca ctgtcatcgg accggccaac
     3421 agaaccggac ggcgttcgcc gcggtccttt tcgtgcagca aaaaggccaa tgcctgcacc
     3481 gtcttgccga gccccatgtc atccgcgagg caggctccga acccccaggc acgcagaaaa
     3541 gccagccagc ggaatccgcg gagttgatag ggccgcaaca caccgcgaaa acccgtaggc
     3601 accggcaggg actccatccc gctcatcccg cccgccagac cgtccagtaa cggatcgagc
     3661 catccccggc ctcgtgcccg cgcgatctcc agaccgcccg ctcccggttc cgtgcccaat
     3721 gccaacctca ccgcttgacg caccggcacc gtgtcggtcc gttgttgtgc gcggagcgct
     3781 tcgcgcacac gcttcagatc cagagtctgc cattgtcccc gcaatgaaac cagcggtgtt
     3841 tcggcgcgct ccagttgcac ggcttcctct ttcgtcagcg gggtgccgtt gaacgtcact
     3901 gaccatgtga cggccacacg cgcttccgaa agcgttccgg tcgtggggtc ttcgcctgta
     3961 agcggggtca gatccgcttc gagcgcgagc gccgatgcgt ggcccggacg ccactgcggc
     4021 ggcaggttca ccgcataccc ggcagcctcc agcactgccg cggccactgt caggaaagcg
     4081 tgggcctcct cctcactcaa cgcgcaaccg aattttccgc cccgcgtttc agcccgcgcg
     4141 agcggcggga agagcagaga ggcctgaccc agcgacagca gaaacgccgc gtcccacgca
     4201 ggggggggga cttctccttc cggcccctct tctgcaagca cacgcaaaaa ccaggcccct
     4261 tctgggccgt caggcacctc cagcgtgaaa cgcggcacct ttccgggctc gccgctctcc
     4321 accggttgcc gccactgccg tagcgctccg gccagagcct ccaactcggc ttgcgtcttc
     4381 cagcgcacct gccgcgtctc gccgcgtaag gccgcgaacc aggcatcatg cggactgtac
     4441 cacggaccgc gttcggcatg tgcgcgggaa agagtggtca ccacactcgc gcgcaccagc
     4501 cggtcgacca actcctccag cgcctccctc ggcgagccgt ttcctgcggc aaaggccgcc
     4561 gggggcaggc gcggcgccag ccgctccagc cgcttgcggt ccgccgcatc cagtgccgga
     4621 atccaacgcg cctcgtacac cttctctgac gtgcggtgca acgcgggcag aaagcgtccg
     4681 cgcgccacca gcgcgcccgc aaaccgaaac agggtggcaa acccgagcag gctctcgccg
     4741 ataaaaagtt cgtcggccag acgcggctcg cgcacgagcc ccaacaacac caaagttgcg
     4801 cgccacggca gaaccacagc cgtcacgcgc cagggatgcg ggaaatctgg cgcgtcctct
     4861 tcccccgctc cgtcgaacac cgcattgttg aaaaagggtt gcgagggcac gggccggaac
     4921 ccgctgcccc aagcgcggct cggcagaaac agttcgagtg tggcaggctg cggcgtttcc
     4981 ccggtccccg gcatgagggg ccgcagcaga tggcacagac gctccgctcc ggcatcgaag
     5041 ggggaaaccg ccaccgcaga acgcggtccg tccggcaaac cggacgcgca ggcctcgacc
     5101 tccccccaaa tatgcagatg ggacgcatgc cagcaactgt gcaggatggt catcaccggt
     5161 caaacagtat acggctcatc ctcctccgcc gcaagatccg gccatgctga aaagcggttc
     5221 aaaatcagcc gcagaaaaat cgccctacat caggttgatc gcatccacat ccacggcaag
     5281 ggttacgccg gatgccaaaa gcggacggcg catgacttcg cgcagcggcc gtgtgatctg
     5341 ccgcgtcgtg ggtgcccgca gcaaaagctg ataccggtac agccctttgg ctttggcgag
     5401 cggtgcggca catactccgg agatgcgcac ggcggccgca ttccctgttg ttttggacag
     5461 cgcgcgcgcg aaggcctctg cgctaaagcg caccttttcc tcggacgcgc cccggaaggt
     5521 caggcatatc aggcgggcat acggtgggta gccgcccgcc tcgcgttcgg ccagctcacg
     5581 agcggcgaac ggttcgaaac cggcatccga ggcggcggcg cagatagcgg gatggccggg
     5641 cgaataggtc tggattaaca cctcgccggg cagctcggcg cggcccgcgc ggcccgatac
     5701 ctgcgccagc agttggtagg ttcgttcagc ggcacggaaa tccggcatgt gcaggctcat
     5761 gtccgccgcg agtacgccga ccagcgtgac gttggggaaa tccagcccct tggcgatcat
     5821 ttgcgtgccg atcaggatgt cggcacggcc acttttgaat tgcgcgagaa tctcgtcatg
     5881 actgcggcgg cgcgaggtga cgtccgcatc catgcgcaag atcttcgcct gcgggaaaca
     5941 ggctgccagc gcggtttccg cgcgctgcgt gccgaaaccg gcgtagcgga atgccggatc
     6001 accgcacagg gggcagacgt cggggacgtg cttccatccg ccgcagatat gacagcgcag
     6061 gcacgcgtcc gcccggtggt aagtgaaggt cacgctgcat gcgtcgcagg ctgcggcata
     6121 cccgcagcgg gggcagagca gcgcattggc gtagccgcgc cggttgagga agagaatggt
     6181 ctgttcgcca cgttcgagac gaagccggat cgcttccagc aggggctcgg agaagagttg
     6241 cgcatgaccg gaacgggcgg tctccagacg catgtccaca acctgtacgc gcggcatagg
     6301 acgccccgcc acacgctgcg tgagatgtgc cgcaacgtat ttgccctttt caacgttgag
     6361 ccaactctcc atcgcgggcg tggcgctgcc gagcactacg gcgcaatgct ccatccaccc
     6421 gcgcatcacc gccacgtcgc gcgcgttgta gcgcggcacc tcatcctgtt tatacgaggg
     6481 ctcatgctcc tcgtccacca cgatcaggcc gaggttacgc accggcgcga aaatagccga
     6541 gcgcggcccc accaccactg tggcctcgcc attgcggatg cggtgccact cgtcgaaccg
     6601 ttcaccatcg cccagtgcgc tatgcagcac cgcaatgcgc ttaccaaacc gtgaggcgaa
     6661 acgctggacg gtctggggcg tgagggcgat ttccggcacc agcacgatcg cgcccagccc
     6721 gcgctgcaac gcatgggcga tcgcctgcag atagacttcg gtcttgccag aaccggtcac
     6781 gccgaagagc aacacgggct tgggcgcatg cgccgagtcg ggagcgcggg cgtccccgcc
     6841 cgccggcgaa acgtctgctg gtctcccggc ggtctgactc gcgccagcat gtgcgggcgg
     6901 ggacgcccgc gctcccgact cggcaccctc cccctgcatc gcgcaaaggt ctgtccgctc
     6961 acccgattcg ataccgcggt tggcttggat atatgcgacc acagaatgaa aatgatttgg
     7021 atcgcgcaca taccggtcga aatactcggg ttgccagagc gcccctttcc gccctaggca
     7081 ccgattgatc cgatgagcag aaaccgattt ccaatcatga acaatctggg cgacggaatc
     7141 cgcttgctcg acctcaatca acgcatgaac atggttcggc atgatggacc acgcaagcaa
     7201 tcgatatttc gtgccgtcgc catgccgaag cacctccgaa acctctcggg caatctctgg
     7261 ctgtttcaaa tagcaagctc cgtgtccagc atcttcataa cgggcaatac gccggatcaa
     7321 ctcttcgttc gcggacggag gtgtcggcac gatgttgacg atgcccaatt cgctcttcca
     7381 cgcatcaatg acagaggagg gcaaactgtc aacaaggcga aacgtaatga attgaacaag
     7441 ccccggataa tcaaaatggg gcacatatcc gcgtgagtgc caatgcgcct ccgtccccgg
     7501 ctcactatga atcatgccgg ggaatttcaa cgacgtcgcg tttgtgccgt cctttgtcaa
     7561 gtcgggcgtc aagtcgggag cgcgggcgtc cccgcccgcc ggtgaaacgt ctgctggtct
     7621 cctggcggtc tgactcgcgc cagcatgtgc gggcggggac gcccgcgctc ccgactcggg
     7681 gcggaccccc gcatcaaccg ccgcgatgat cagcttaagg gcggaggctt gttcgggcat
     7741 gagtgccaag gggcgggtcg gcagcacctt gcggttggcg agaggatcgc gacgcacggc
     7801 gcggcgctcg atcaccgccg cgcccttcgc ggccagagtg cggagcgtct gcggggcgca
     7861 ggcgaactcg cgggtcagac tgtggagcca gccgccatcc acacggcgca ggttttccag
     7921 cagttcgttt tggcgtgaag tcagcgcgaa ctcccccggg cagggcgtga catagaagcg
     7981 ctccttgacc ttgatatcgc ggttgcgcac cgcagcgggc accatgcagc gcagagccgc
     8041 ctcgaacggc gaacaggtat aatccgccat ccagcgcgcg agcgcgatca gcgttgggga
     8101 gaggaacggc acgtcatcct cgatagcgag gatcgggcgc agccgccggg tgcggattcc
     8161 atcggtcttg tcagacccgc cggacaaact gaagagttcg ggaggtccgg gttccaacgc
     8221 ggtgtcctcc gcgatctcca cgacataggc gtccagcgta cgcttgccga agggcacacg
     8281 cacacgctgt cccggccgga ggcgcggcgc gagcgcgtcc ggaacggcgt aatcgaagag
     8341 ccggtccgcc gaaatctctg tggccacacg cgcgagcatc cgtggtcttc ccgtgatcaa
     8401 aaaagagggg agcggcttgc cgctccccct ttttcctttc catgacaaaa cgcgtaaacc
     8461 tcagcctccg ggggtcacag aggttttcgg cgtcagatac ttgagctgct gggcgtcctg
     8521 ctgcagcgtg gtgtcgaact gctggttatt gtagatcacg cgcatctcgc ggtacttgcc
     8581 gcggcccttc aagatcgcgc cacccttgcg gaccatgacg aagcctttgt tggagaaggg
     8641 tttcggatct gaggaagaga agttcaactt cgtgttggcc ttgttgtcgt tgtacttggc
     8701 ggccagtgag ctggcgtcga tgttccgcgt gacgagaatg gggatgacgt cctccatctc
     8761 gtcgcgcaga ttgcaggcga tggtccacat gttgtactcc tcggagagat tacgcttgcc
     8821 gcaggagggg atgccggcgc cagccagttt cgagtaatcg aacccctcgg ccacgggact
     8881 ccagtccgtc gtgccggcat tctcttcgtc gtacaggtag gcgaaatact tggtgctgtc
     8941 cgtggaagcc gtttggcggt cctgatcgtc ctgggagagc gtgccggtgg ccttggacat
     9001 ctcgtccggc catacattgc caagaccgag cggctcgcgc tccatgttgg ccgaggtgat
     9061 cgccacgaag atatccttgc cgcgtgtgcc gaccgccgcc atgttggcgg ccatcaccgc
     9121 gttgccgatc gccgggaaaa gcgccgcgga gaggatgccg aggatgccga tcacgaccag
     9181 caactcgacg agggtgaaac cgtctttttg atgtcttgtc atttcatgtc tccttcatgc
     9241 aggccggtcc gcggactgcc ttgtctctgt tgtataacgc cattgtgcca ccagaagggg
     9301 ggacacgtca agcgcgtgtt tgggggatgg tgtttttatt ttccgccaac acgccctcgt
     9361 cccttgtcat gatgccggcg ccgggtcagg gggcgcgctc gcgcgccgcg cgttcggccg
     9421 cgcgctgggc catccgccga cgcgcctcgg ccgcgatcag ctcgcgagcc tttttccgtt
     9481 gttcggcggt caggcggtcg cggatcacca ccaactgccg ggtggcgagt ttggcctgtt
     9541 cggtacggtg ccgcccgatg cgctcgatga tggccatgat ctcgtccacg cttgcgccgg
     9601 gttccgacag cacgttccgg gccagctccc cctggcggag cgcctcgcgg ttgatcgtct
     9661 cctccagccg ttgggcctgg gcttggatct cctccagttc ggctttgagc tgttcagcct
     9721 gagcgccccg taaccccact tgttccatga actcaggggt cagaacgtgt tttccgaccc
     9781 aattgctgcg gttgatggtc cggggcgggc gcgggcgccc ggctctcggg ggcggttgcg
     9841 cctgaaccca acccgcaacc cacaccaagg cgatcaatcc gacccgtata ctccgtttca
     9901 tgcctaccct ttcctgtgag aaacaggcaa cctgtttctc gtcgcaacgt taccacgaga
     9961 atgaagggac taacgtcccg ccggatgatt tattgccgca tccgttttca cccggagcgg
    10021 tgctccggat agtcggcttt cttccttctc cgtcaagccc ggtctttggc gtgtcgcgtt
    10081 ctctccctca gaggtcgaac agcgtgagct ggcccgggat gtcgtcatcg ttccggcgtc
    10141 gactccggcg ggcgagtttg gggaggccag actccagttc gccttcttcc agattcgcga
    10201 ggatctcacg tgaacggtcg atcacctcat ccggcatgcc cgccagccgc gccacatgga
    10261 tcccgtaact tttgtcagcc gtgccgggag cgatacgccg cagaaaaacc acggtgtcgc
    10321 cgtgctcccg cacttgcacg gtgtaatttt tcacgccgct cagggtcagc gccagatcgg
    10381 tcagctcgtg gtagtgcgtg gcgaacaggg ttttggcctt cacacgcggc gtgttgtgca
    10441 ggtactccgc cacggcccaa gcgatgctga tgccgtcgaa ggtgctggtg ccgcggccga
    10501 tctcgtccag aacgatcagg ctgcgcttcg tggcattgtt aaggatgttg gcggcttcct
    10561 gcatctccac catgaaggtg ctccggccgc gcgccagatc gtctccggcg cccacgcgcg
    10621 tgaagacgcg gtcgagaaga ccgatacgcg cttcgcgcgc ggggacgaag gaccccgtgt
    10681 gcgccaggat cgcgatcagc gccacctgcc ggatgtaggt ggatttgccc gccatgttcg
    10741 gcccggtaat caacatgatc tggttgcggt ggccgtcgag ccgcgtgtcg ttgggcacga
    10801 acggcccggc gtcgggaagt tgttccacca ccggatggcg tccgtcgcgg atttccagcg
    10861 tgtcgtcctc tgtaatttcg ggccggacat atccgagcgc aagggcgcgg tcggcgaaaa
    10921 cggccagcac atccagatgc gccagggcgt cggcggtctc ctgaatcgcg gcggtctgct
    10981 ccaccgcttg ttcgcggatg ccgcagaaca actcgaactc cagcgctgtg gcgcggtcct
    11041 gcgcgccgaa gatcttctgc tcgtaggtct tgagttcggg tgtgatgaac cgctccgcgt
    11101 tgaccagcgt ctggcgtcgc tgatattcgg gcggcaccgc gccggcctgt cctttggaca
    11161 cctcgatgta gaagccaaac accttgttgt gccgcacttt gagggttttg attccggtcc
    11221 gctcctgctc cgaggcctgg tatgcggcaa gccaggcgtg cccctctgtg gcgagggcgc
    11281 gcagctcgtc gagttccgcg tgataccccg ctcggatgat gccgccatcc ttcagccccg
    11341 cggcgggtgt ctctgcgatc gccgctgtga tgaggccgac cagttccggc agggcgtgga
    11401 ggcgctcagc cagctctgcc agcagcggat cgcctgcggc cgccacccgt tcgcgcagac
    11461 cgggcacggg atgcagggag agggccagcg cgttgagatc ccgcgcgttg ccgtgcccgg
    11521 caccgagacg agcgatcagc cgttcgagat cccgcaccgt gccgaggctt tcgcgcagct
    11581 cgttcagcag cgtacgctgt tttgtgaggc ggtccactgc atccagccga cagcggatcg
    11641 cctctgcctg acgcagcggc cgcgtgagcc aggcgcgtaa catccgtgcg cccatcgggg
    11701 tccgggtgtg gtccagcacg cccagcaggg tcgcctgcgg actctggccg cgcggcggaa
    11761 tcaagtcgag attcaggcag gtgttctcgt ccaaggtcaa aaactcgtgt gcgcggcgta
    11821 cgcgcaacgt gcggatgtgc cccacgggat ggcgcagctc ctcatgcacg tagtgcagca
    11881 gcgcgccgac ggggcagacc tgcgctgcac ggccatcgca tccgtacccc tcgagcgagt
    11941 gaaccccgaa gtggcgaagc agccggtcgt acgcacagtc gtattcgagc agccaatcgt
    12001 caatctcggt gaccataccc ggcgcatccg gcgcaagaag agcggccaga cgcggatcgt
    12061 tgcgctgatc gcggccgacc aggcactcgc tcggcgcggc gcggttgagt tgctcggcca
    12121 gcacggcggc gtccggcgct tcctcgaccg tgagcgcgcc ggtcgagaga tccagcaacg
    12181 cgaggccgag ccctttgccc gcgtagaccg cggccaggta attgttgcgc gcggcatcca
    12241 gaatctcgtc ctcggtcacc gtgccggggg tgaccacgcg cgtgacctcg cgccgcacga
    12301 tacccttggc ttgggcggga tcctccatct gatcacagat cgcggccttg cgcccagcgc
    12361 ggatcagctt ggcgagatat gcgtcgagcg catggtgggg caccccgcac atgggtatgc
    12421 cggtgcgccg ggtgagtgcc acaccgagaa tcggcgcggc ctcgcgcgcg tcctcaaaaa
    12481 acatctcgta gaaatccccg aggcggaaca agagcagcac atcctgcggc agttccgctt
    12541 tgattctgcg gtactgccgc atcatcgggg tcaatttgtc accagccatg tgaatcccac
    12601 tcctcacctt ctgcatcaca catgtgaaaa acaggcttga gggaagtttc gcaagttaca
    12661 ttctgcccgt caacaccgga agggcgggac cggcttggcg ggattgacag gcagggcggg
    12721 ataggcgatg atccttgaag acgataaagg aaacacgttc agatgattgc ttctcccgtt
    12781 tatctggcct ccgtggtggc gctggcggtt gccgttttgc tcattctgat catcagactg
    12841 aagcagcaac cgtttgtttc actgctcctc gtgagcatgg gcaccgctct ggcggcggga
    12901 atgccgccgg agaaggtgat tgcgtgcatc gagaagggga tgggcggaac gctcgggttc
    12961 atcgcggtgg tggtcgggct gggcgcgatg ttcgggcagt tgctggaagt ctcgggcggt
    13021 gccgagcggc tgacgctcac gctgttgcgc gttttcggca aggagcgcgc aacgtgggcc
    13081 atggcgctgg ccggatttct ggtggcgatt ccggttttct tcgacgtggg gttcatcatt
    13141 ctcgtgccgc tggtctacac cctttcgcgc gagaccaaga aatcgctgct ctattacggt
    13201 atcccgctgc tggcgggtct ggcggtgacg catgcgtttg tgccgcccac gccggggccg
    13261 atcgcggtgg cgcaactggt gggggcggat ttgggacgtg ttattttcta cggcgttttg
    13321 atcgggctgc cctgcgcgat cgtggcgggg ccggttttcg ggacttggat cgaccgccgc
    13381 attcacgcgg cggttccggc ctatatggag acggaggccg cgaagaaaca atgggacgag
    13441 aaggctctcc ccggcttcgg ccgcgtgttc ggtatcctgc ttctgccact gttcctgatt
    13501 ctgctgaaca cggtgaccca agccgtgctg gcggaagggg aatatgcaac ggttcgacac
    13561 gtcttcgcgt ttatcgggca cccttttttc gcgcttctgg cggccacgct gctcgctttc
    13621 acctttctgg gcacctgtcg cgggctgacg cgcgagaagg tgaacgaggt ggcgacgtcg
    13681 gctttggctc cggcgggcct gatcattttg gtgaccggcg cgggcggtgt gttcaaacag
    13741 gtgctggtcg acagcggcgc gggcaaagcc ttggccggga cgctagccca tacgggtctg
    13801 catccggtct ggaccgcctt tctgatcgcc ttgctcgtgc gggccgtggc cggctcggcg
    13861 acggtcgcga tgctgacggc gggcgggatc atggcgccac tgctggctgg acagcgggtg
    13921 gagcccgcgc tgatcgtgat cgcaatcgcc tcgggcgcca cggcgctctc gcacgtcaac
    13981 gacagcggct tctggctggt gaaccggtat ttcggtctga gcgtgaaaga aacgctccgc
    14041 tcgtggaccg tgatggagac cctcatcggc ctgatcggcg cgaccttggc gtggctgttc
    14101 agctatgtgg cctgaccgcc gcagcgttcc gttacagcaa cccgcgcttg atcagctctt
    14161 ccgcgatctg caccgcattc tgcgcggcac ccttgcggat gttgtccccg cagcaccaga
    14221 gcgccagccc gttttccaca ctcggatcgg cgcggatgcg cccgacgttc accgtatcgc
    14281 cgccgcccgt gttcagcggc atgggatact gcgccgccgc cagatcgtcg atcacccgta
    14341 tgccggcggc gttcgccaga atggcgcgcg cctcctccgg tgccagcggc gcactgaact
    14401 cggcgtgcac ggccaccgaa tgcgcgtaga aaaccggcac gcgcgcgcaa gtcgccgcaa
    14461 tgcgcaggtc ggggcagccc agaatcttgc gcgcctcgaa agtcagcttg gcttcttcgg
    14521 aggtgaaccc cggcatctcc ggcttggcac cgccgatctg gggcagcacg ttgaaagcga
    14581 tcggatgagg ataggcgcac acctgcggct cgttacccgc ggcataggcg cggacctgct
    14641 gttccaactc ctcgacagcg gcgcggcccg tgccgctcac ggactggtag gtgactgcgg
    14701 tcatgtgccg cagcggcaca cggcggtgca gtggcgcaat gcccatcagc gtaataatcg
    14761 tactgcagtt ggggttggca atgaacccct gatgcgcggt cagggcctcg ccgttgatct
    14821 cggggatcac cagcggcacg cgcgcgtcca tgcggaaatc gtcgccgttg tcgatcacca
    14881 cgcagccgcg ccgaaccgcc tcccaaccga actgcttcga cgcgcctttg gagccttccg
    14941 tcccggcgaa cagcacgatg tccaacccgt cgaaggcctc aggccgggcc tcgatcacat
    15001 ggaaggtttc gcccgccacc tcctcatccc gctcacgggt ggccatgatg cggatctccc
    15061 gtgcgggaaa cttccgctgc cggagcacct tgatcatctc cgtgcccact gcgccgatgc
    15121 ccacgagacc tacgctgtac tgcttcattc gcaatccctt cgcttgcttt caccggtcaa
    15181 tcagcgcgcg catctccggc gtgatctcga ggatctcgaa atccttgaac cacatcttgc
    15241 cgtttgcgcc gccgtggagt tgcagggcca ccttaccgct cttctcgcaa agcgggtcga
    15301 ggatgtccac cgtgcgttgg ccgttgagga tcgagaggat ccggtcgccg tacgccaccg
    15361 tgcaggtcgt gttccatccc ttggggttcc ggacctgcgc gatgaactcg tcattctgcg
    15421 ccacccaacc gcgtcccttg gtcttgtcgc ccgcagtatg ccacagggcg gaatccttgg
    15481 cgtttccggc gatctcgttc tggaacccct tgagcagcca cggcgtgtta acctcttcgg
    15541 cgcggaaata gagcgcgctg ttgccgccga agagttggta agtcacgcgc acgatgaaat
    15601 cgctgtagtt ccggtccgag accacgagcc cgtcccgctt ctctccctcc ttatgctggc
    15661 cgatgagaat gccctcttca tgattgaacg accaatcctc cggcagcacg aaacgggcgc
    15721 cgcggagcgc gtaccggcca tcctcaccct tgacaaagaa gggcgtccac cggctcgtgc
    15781 ccagatcccg gatacggatg ttgcgccagc gggcgcaggg tttgccgtcc ggatttttga
    15841 tcgcgtgcac ttgcaggccg atgaacccgg cgagcgcggc gtcgtcaaaa tagttcacga
    15901 ccggcacgtc gttgagccaa gtgcggatcg agggcccgac gcactggatc tccatccggt
    15961 tccaatcccc ctgcttatag gcggcaaccg atttcgcggt cacctccgca ggcgtgttat
    16021 ccagccaagt ccgcttgaat ttgaacccgc gccgcccctc gtcgtaaata cgggccgtga
    16081 ccggcgagcg ccccgcgatt tccgcctgat acccgtaaac cgaatcggat ccgtctttgt
    16141 gcttgcgcgc cgcggaacgg aactgcaccc cggagttgcc gccctcgaac ttgaactcca
    16201 gacgcaggat gaaatccccg tactcttttt cggtgcacaa gaacgaattg cggcccgcgt
    16261 cgccgaaaca gccgacgatc gcgccatcct caacccggta aggcgacttg ccgccccgct
    16321 ggatccagcc gttcagcgtc ttgccgtcga acagcgtcac gaacccctct tcccccgccg
    16381 gcgcgtccgc tgcgatcccc gccgccagaa taccggccat tacccacccg atccattgac
    16441 gtttcatctt gtcctccctt ggccgcaagt atagtacacc ccctgtgcgc ttttccagcg
    16501 caattgcctg acggaaagcg gcttattccg tcagcgcgta cgacttaggc ctgcttactc
    16561 gttgagctga ccctgataca cgcagcgccg gactttctga atcgaggtgc gttccagcgg
    16621 ttcgcggcgc accttgattt ttcgcggatg cttgtactcg gtcagacatt cgcattgtgt
    16681 gcgcacccgt tcgcggatca ggcgttcgac cgtctcccag gggggctcca ctccttcgtg
    16741 ggccgccgcc actgcctcga ggtcgggcac cacgatcgtg cccacacgct cgccgggtat
    16801 tccgcccacc gtgtagccga tcaccaccac gtcggcgatc agcggatccc gcgacagcac
    16861 gttttcgacc tcttcgggat agatgttttt gccttcgcgg ttgacgatca gcgccttttt
    16921 gcgcccgcag atcgtcaggt acccttcggc gtcctgcacg gccaggtcgc cggtgcgcag
    16981 ccactcgccg tcgaacgcct cgcgggtggc ctcgaggttt ttgaaatacc ccttcatcac
    17041 gatcgggccg cggatctgca gttcacccac gccctgcgga tcgggatccg cgatccgcac
    17101 ctcgatgttg ggcagttttt tcccgatcgt ccccacacgt gagtttttgc tgttggtgat
    17161 ggaaaccacc ggcgaacact ccgtgaggcc gtacccctcc acaatcggca cacccagacg
    17221 ccggaacccg ttgagcacgt acaaagggca cggcgccccg ccgacaatca ggaaacgcaa
    17281 cctgccgccg agtcctttca gcaccttttt gcctacccag caccccagcc ccagccggaa
    17341 tagaagtcgg gcgatccggt tccgccgcag cttgtcgtcg attttgttga acatcttttc
    17401 gaccagcagg gggaccgcga tcaggatgga gggccgcagt gtccggatgt cctcccctat
    17461 gcttcggaga ctcgcgataa aagacatgcc gcagccccgg accagcggca ccacaaaatt
    17521 cgccgtgaac gaaaaagcgt ggaacagcgg caacaccacg aggaagtcgt cctccgcagt
    17581 gatgacgtcg ccgatcgcgt cgaaacagcc gaaagcgtca ctgcaaaaat tgaggtgcgt
    17641 cagcatcgcg cctttgggcg tgcccgtggt gcccgaagta tagatgatgg acgcgacgtc
    17701 ctgggggtcg ggccggtggt ccgcatacca gaggaggggt tcattcgcca cgcccgcccg
    17761 cagcgtctcg taatcgtaac agggccgtcc cgcgatcggc gcgaggcgct cccccgcgcc
    17821 atcggtcacc acaatgaacc ggacctgcgg caggtcggtc agaatcactt cgagcatgtc
    17881 gagatggcgg gtgtcggtca agaccgccac cgcctcggag tcgttcagga tgtacgacac
    17941 ctccgcagcc cgcaacttgg gatcgatcgg caccaccgcc acgcccgcgc ctgcagtcgc
    18001 cagataggtc tcgatccact ccggcccgtt ctccattatc atcgcgacgc gctcggtgat
    18061 cggcttcaac cccagacgcc cgaacgcctc ggccatctgc cggacgccca gcatcaggga
    18121 ttcatatgta cggctctgcc aagcctgatc ccggtgatag cgcaatgcga tcgccgccgg
    18181 ccttctggat gccgtatcct caaatatttt tcgaagagtc atgtccgccc agaatccgtc
    18241 caagctggtt gtgaaatgac acaatagccg gaagtgtcat aaaagccaat cccaaaaagg
    18301 at
//
LOCUS       DAOSRB010000037        18296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3199_length_18296_cov_6.176525, whole genome shotgun sequence.
ACCESSION   DAOSRB010000037 DAOSRB010000000
VERSION     DAOSRB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18296
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_18296_cov_6.176525"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLZ74_03480"
     CDS             <1..309
                     /locus_tag="PLZ74_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97416.1"
                     /translation="VSRFQGFIQIGVGIGIAIAIGIDHLTTGRFFGFQGFKVSGFQGF
                     WFQGFIQIGVGIGIAIGIDHLTTGHFLGFQGFKVSGFRGFHLSRPTGVSCPVSRRLVI
                     "
     gene            357..1175
                     /locus_tag="PLZ74_03485"
     CDS             357..1175
                     /locus_tag="PLZ74_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR97417.1"
                     /translation="MITREPVYTDAPPNAIAGLGRAIVLGLGDIGRAGSMTAQALAGM
                     PLLLFCARRRRECVRQMFHMGIKTLPVISVVGMFTGMILGLQVGLELRRFNQEIYIGS
                     AVMVSLLREMGPFMTGLILAACVGSSMAAQIGTMTVNDEIAALEIMSIDPVLFLMTPR
                     IAAMVFMTPVLSFYTCVLGVIGGGIVGLTQLNVPWDQYIVMAMDIARLRDLYVGLFKA
                     LLFGIVITGVSCYEGFTTTQGAVGVGQATRRSVISSFLLILILGYIVTRMFYDL"
     gene            1172..1924
                     /locus_tag="PLZ74_03490"
     CDS             1172..1924
                     /locus_tag="PLZ74_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR97418.1"
                     /translation="MIRFDHVTKQLGGRLVLDDLSFEIPKGEIFAIVGPSGTGKSVTL
                     KHMVRLMTPTRGAVWIDGTEISAVDGADLARARERFGYLFQGGALLGWMTVEENIALP
                     LREKTRLSEAEIAERVAKALCAVGLEEDGDKFPSEISGGMQKRAGLARAIVREPEIVL
                     YDEPTSGLDPVTARTIDKLIRRLNKELGITSVVVTHDLQGALLSADRIAMLTGGRFVE
                     VSSPHAFVAADQPDVRRFLDAQFISKKGIEGM"
     gene            1973..3085
                     /locus_tag="PLZ74_03495"
     CDS             1973..3085
                     /locus_tag="PLZ74_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOR97419.1"
                     /translation="MALKRNREVFSEVIVGLFMVAVLGLLAYFTIIISGIDILNGKNK
                     GPVTVLFTDVGGLKVRDSVILRGMTVGSVSEMHLENSGVRVTLLIDGGVRLKEGYRIS
                     VNTGSLLGGNYLLIEEGEGAEVPRETVLRGETPSQWMRDLGVIVANLREATEGSHLRN
                     TLTNLDVAVASIRAAVTRVEEGRGTLGKLFSDDMTLYNDLSNTVASLRSVAVKIDAGE
                     NSLGRLINDDGNVYTNLSASIANLKSISDRLEKGEGTLGKLLSSDAGIYDDLRETVEN
                     LKTVSARLEKGDGTLGKLLKDDNKVYNDLDATVANLREVTDRLVRGEGTLGKLSKEDE
                     LYSDTQGLIRDVRQTVDNFRDTTPISAFASLIMGGL"
     gene            3101..3628
                     /locus_tag="PLZ74_03500"
     CDS             3101..3628
                     /locus_tag="PLZ74_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97420.1"
                     /translation="MLYTLGEMAEKLNRSPVVLRGLQRSFKLPVYRGADYSESYLAFL
                     DTVLALRTLNVAEKTLLKLWRIERKLMRMLHADTTGSPTWFLDSCGSTRNRKRRLLLS
                     HFDIGTTLPSGAIQLGFAFSEPQRERFASRGMGEDVLAVLNLYTGLHNEISDTVRAEM
                     PRLFSALSLSKWFGR"
     assembly_gap    3640..3739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3991..5453
                     /gene="guaB"
                     /locus_tag="PLZ74_03505"
                     /pseudo
     CDS             3991..5453
                     /gene="guaB"
                     /locus_tag="PLZ74_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
     gene            complement(5489..5734)
                     /locus_tag="PLZ74_03510"
     CDS             complement(5489..5734)
                     /locus_tag="PLZ74_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOR97421.1"
                     /translation="MKSETGTPATAGKTRNLLDLQDGETGRIRSNRDRKSAEMGLFAG
                     VRVTMFRNRKSEHSVVVGAGDARFLVSRPIAEHIELA"
     gene            complement(5724..7976)
                     /gene="feoB"
                     /locus_tag="PLZ74_03515"
     CDS             complement(5724..7976)
                     /gene="feoB"
                     /locus_tag="PLZ74_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOR97422.1"
                     /translation="MRDDLTIALAGNPNTGKTTLFNLLTGAHERTGNWTGVTVDTAHA
                     AFTLNGKTARVTDLPGIYSFSPHTQDERVARDFLIQQKPAVIVNLVDAVNLERSLYLT
                     AQLLDMRLPLVVALNQTDVAERYGIHVDADHLASHLGCPVIPMVASKGVGLEALKQAI
                     CHVAETRSLPTTRVQYGDAEPSVRRLQVCLIVAADDAGVDSRWLAIKLLERDPFAREL
                     TAGLFDELAEEECARIARHTGQKPEEAVMDARLGFIRGLARDVVQRKRVSRRRVSDVA
                     DRLLLSRPLGIPFFLGVMYVVFWLTVRLTQPLVDFTDLALNALLVNAPRIWLARLGMP
                     EILGSLLCDGVGAGLTTVATFMPPIFMIFLCLSLLEESGYMARAAFIMDRFLNRIGLP
                     GKAFIPLLVGFGCTVPALLATRTLEQRRDRLLTMLIAPFMSCGARMPVYAIFAMAFFP
                     NQGNIVIFSLYLTGVVLAILSGLMLHRTLFRGGAASAFVMELPPYHLPALRGCLEHVW
                     ANLQSFLVRAGQVILTIAILLSLANALFEKAYRNPETAFAHRSAAGRVLTVPLRPMGV
                     TGDNWPAAAALLTGLMAKEAIVGTLEVLYFQTEKTAPDSRKPFDLRAELRAAGSALFA
                     GFSGSAATADPDRSADPAARQGLLSTLRRNFASPTAAYAYLLFVLIYSPCVAALIVLA
                     REAGWRWMGFAVTYQSLLAWLTATVFYQCASFPAAPLRSVGWLAFVTALTAAGIALLA
                     AYGKRIAHEV"
     gene            8156..8929
                     /locus_tag="PLZ74_03520"
     CDS             8156..8929
                     /locus_tag="PLZ74_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HOR97423.1"
                     /translation="MSQPVRIMPCLDMRDGRVVKGVRFVDIRDAGDPVACARAYCEKG
                     ADELAMLDITATVEGRATLLQVVRNVAEAVTVPFTVGGGISDVKTARAVMEAGANKVS
                     VSSAAFRKPALIPELVAELGAERVTVAIDVDRNAALPSGYEVYIDGGRTATGHDAVEW
                     AKRVDGLGVRVILPTSKAGDGARTGYDLPVIRAIRQAVSAEVVASGGAGTLEHFHEAV
                     EAGATILLAASIFHFGIIGIPELKAYLRERGVKVCGGEA"
     gene            8961..9542
                     /locus_tag="PLZ74_03525"
     CDS             8961..9542
                     /locus_tag="PLZ74_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOR97424.1"
                     /translation="MKVVAFNGSPRQDGNTALLLRKVLAPVARSGIDTELVQVGGHAV
                     HGCTACMRCMTARNRQCAITTDRVNTWIAKMIEADGIVMGSPSYFSGMTPELKALLDR
                     AGLVAVANGRMFARKIGAAVAAHRRGGAVNVVDAINHLFLMSRMIVPGSTYWNFGVGL
                     EREDVADDAEAMENMHDLGETLVWLLKRLHADT"
     gene            complement(9585..10256)
                     /locus_tag="PLZ74_03530"
     CDS             complement(9585..10256)
                     /locus_tag="PLZ74_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97425.1"
                     /translation="MRKTVAFLALGLALLAAVMAFQRAIREEGRRIRSVFHQALAEPD
                     GLRERQADLLRKAETLNVQLATLNRRLSHLESRAPKETRAAPTGSAAPHSPAALRTDE
                     FRAAIGHLNALPDLLTELTAYLDQAFEHLEETVTVTAVPEDFAERLAAMEKRLADIDS
                     YFTPLYAFLGLVYDPANEAVLAAYPTLDKRISALDEKLVQLQRDLAALRESLEPRTKE
                     PRPVR"
     gene            complement(10322..11776)
                     /locus_tag="PLZ74_03535"
     CDS             complement(10322..11776)
                     /locus_tag="PLZ74_03535"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOR97426.1"
                     /translation="MKKSYWVTLLRFLVGWHFLYEGLWKLLSGSWSAEPYLSSSEWIA
                     AKLFHTIAETPALLKVVDLLNIWGLTLIGLALILGVAIRFASISGIVLLALYYLAHPP
                     FTAPAGGESHLLWIDRNVIEAFALAVICVIPSYGLGALFQQYRNRRESPIAFDRREFL
                     KNLSSLPVLGAFAGAFIEKHGPVWEKEHLKSIADAMTGPTLRTFESKDISKLKKPIEA
                     FGQIGSTRLSRMFMGCNLIGGWAHARDLIYVSQLVKAYHEDWRVFRTLHMAEAAGMNT
                     IILNPGLFRVMGDYLKKEGGKMQVLSDCAAGGNPIKGAEISIKFGAQMLYIQGEMTEK
                     YIIREKNYKIMEDALAFMRKEGYQAGIGAHSLEAVKECVDRGLIPDYWVKTIHPINYW
                     SANINGGTRHDNIWCTNPKETIAYMKERPEPWIGFKTLAAGAIRPKDGFPFALKAGAD
                     FLCVGMYDFQLVDNVNLFNEIFPSCQERERPWRA"
     gene            complement(11807..12673)
                     /locus_tag="PLZ74_03540"
     CDS             complement(11807..12673)
                     /locus_tag="PLZ74_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOR97427.1"
                     /translation="MRIRTLYLCLLAGFMTCHSAVAEPLRLATFNIRLPADKGVNAWS
                     NRAPRVIALVKRHGFDLVGLQEATAMQFDEMLAALPGWAMVGVCRDDGKRKGEATCIL
                     YRADRFTVQKSGTFWLSETPEVPGSKSWKTACTRVCTWALMTDTKNNKPFAFFNTHLD
                     HISVEARANGMKLILERMQTLVPQGWPIFLTGDMNCNETSEPVQLAGKVLRDAVRHTQ
                     TPHQGPWRTFTGYRYRPDAEEPKAPAKDAPRIDYIFVSEKVTVRDVTTHNDAENGNYP
                     SDHFPVSATVVF"
     gene            complement(12757..15654)
                     /locus_tag="PLZ74_03545"
     CDS             complement(12757..15654)
                     /locus_tag="PLZ74_03545"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOR97428.1"
                     /translation="MNVQDKRRGAISQPARLTATTLFLLTGFTGVAFPCFSHGAARVK
                     EITLEMPTYGFSAPNPIPEPETRVDRQIWPYFKFEGYTAVATTQSWTCVRLENDAVEV
                     DVFPQIGGKVWSARDKRTGRDFLYRNRVVKFRNISTRGPWTSGGIEFNFGMLGHAPTA
                     STPVDYACRTNSDGSASCFVGVLELITRTRWCVEIRLGASDRFFTTQTTWHNASGLPQ
                     PCYQWMNAAYPCGGRYVFPGTARLEHDGTASPWPRDASGLDLSVYTNNAFGLSKSYHV
                     VNGDTGFFGVWWDAHQFGSMHEAARFDRLGRKIWIWSLSRSGAIWEDLLTDEDGQYVE
                     LQSGRYFNQPSHGSHRTPFKYPSFPAGSTITFSERWSPVTEARILEERAAESGTVSRP
                     LATPADFDWGTVYGQTLQAEQDVNLKDYTAAEKTLRAVLAKEPHYLPALVCMADLLLH
                     CGRPAEALAFSERALAVDAYHDKANHLSGIVFAERGDHANALERFGAAALSPLFRSAA
                     ITRIAALECAAGRPDRMLEAAERALEANPLEFSALHLRLVALRLLGREDEHRAACAAV
                     LDRFPLFHPARCEQDRAAFAAGIRCELPHEEILETAHLYAAAGLFGEAAEIAALASGR
                     FLAAVARAYWLARTGDETAARNALADAERLDTAFAFPFRPETLPILEWAHAHSPSWKP
                     RYALALLLRHFGNDDRARALLNETDADDVPFLLFRARLRDGPSALADIRKAQTFGENR
                     YAVKAEWRAHAEANDWRQALGAAQRLLAAHPEDNDAGILLAQSHLRLGDPARCLETLD
                     TVRFLPSEFTSITRRVYREACLAQAVAAFRKSDSAVTRDAVRRAGIWDEKLGVGKPYA
                     SEIDMRLELWLFAQLEQGVTRADLLDRVAAGFAPSRPARTANALDLLTALACRERGQS
                     AKADAVAAAFGTDAVGVWCRAVFEGDRATASERLPAASSDENVRLLAALLL"
     gene            15816..16025
                     /locus_tag="PLZ74_03550"
     CDS             15816..16025
                     /locus_tag="PLZ74_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97429.1"
                     /translation="MRNAGLAGWIMLCGMVSGYPALAAGRVILRPVAAPPPVEVAARG
                     FQKDHEAVTGVRLEIMTAPDAATDV"
     gene            16045..>18296
                     /locus_tag="PLZ74_03555"
     CDS             16045..>18296
                     /locus_tag="PLZ74_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97430.1"
                     /translation="MPDQVDVTLTERIKQFALEGLNADLVGVANIGRFHGAPTMMSPQ
                     GILPSARSVIVMAVHHPDAAIELGGEEHPQQIGPYRIQYAMNMRLDEMSYRMGRFIES
                     LGHTAVPIVSSNIWRYKGYKELREHFAPDMSHLHAAVAAGLSEFGYSGLSITPEFGAR
                     QRYVSVITDAALTPSPLVEPGSVCDMCMLCKKVCPTAALTRELDGWNEVEIEGKVYRY
                     VRKNLWRCAWGEHFDLDLDLEIPEKVDEAVILDHVKRFGRRGGEMGCCLKVCVPKMRR
                     VLDRTYCTGPRRRRDVMPPPGPVPREVTERLHALTRAHGVSCILTVPVDTLRAAGVTV
                     EDSKAYLPGVKSAVIACLAADSALVRETTGTDKELALEITLVSGGLRETAEKLVLQTG
                     YDLARELERAGYTATGGTELSESDVARAVSGVPEGQTLFTYTVLTDVELPSTGLAFRA
                     PPVESAEDLTEALKAEAGRLGADVCGVTSIGRLQGVAQQVRRVFEGEEVLDAQDTSQL
                     FTPYRPVITSRRRQIPDPATLLPGSRSVFVFGIALPHGSVDITARTPAEAVGPYAFAL
                     YENLNYLRRVALRLALWLSRQGVKAFVSSDLLGTGSMTASPRGAVPDLFSNRFEAWAA
                     GLGRFAKAGFLVNPRFGTNVRYMAVVVDRELSEDALQNGAAPETCAACGERCVAACAS
                     RAFGETMTVEAEGVRDTFRTIQTVRCDWAKRYSLIADEGCGFVGWSLNLPPPETITPE
                     ALAEGLRQQPP"
BASE COUNT         3394 a         5228 c         6101 g         3473 t
ORIGIN      
        1 gtttcaaggt ttcagggttt catccaaatc ggggtcggaa tcgggatcgc tatcgctatc
       61 gggatcgatc atttaaccac gggacgcttt tttgggtttc aaggtttcaa ggtttcgggg
      121 tttcagggtt tctggtttca gggtttcatc caaatcgggg tcgggatcgg gatcgctatc
      181 gggatcgatc atttaaccac aggacacttt cttgggtttc agggtttcaa ggtttcaggg
      241 tttcgtgggt ttcatctgtc gcggccgaca ggagtgtctt gcccagtatc ccggcgtttg
      301 gtaatctgat gcaacaaaag gtttaacgca ccggcaaagg ggataggagg gttttcgtga
      361 tcacgcgcga accggtttat accgacgcgc caccgaatgc gatcgcagga ttgggacgcg
      421 cgatcgtgct ggggttgggg gacattggcc gggctggcag catgacggct caggctttgg
      481 ccggtatgcc cttgttgctg ttctgcgccc ggcgccgccg cgaatgcgtc cgccagatgt
      541 tccacatggg tatcaagacc ctgcccgtaa tctcggtcgt cggaatgttc accggtatga
      601 ttctgggact tcaggtcggc ctcgagctgc ggcgctttaa tcaggagatc tacatcggtt
      661 cggcagtgat ggtctcgttg ttgcgtgaga tggggccgtt catgaccggt ctgattctgg
      721 cggcgtgcgt tggatcctcc atggcggcgc agatcggcac catgacggtg aatgacgaga
      781 tcgcggcgct ggagatcatg tccatcgacc ctgtgttgtt tctgatgacg ccgcgtatcg
      841 cggcgatggt gttcatgacc ccggtgctct cgttttacac ttgcgtgttg ggcgtgatcg
      901 gcggcggcat tgtgggcctg acgcagttga atgtgccgtg ggatcagtat atcgtcatgg
      961 ccatggatat cgcccgcttg cgcgatctgt atgtggggct gttcaaagca ttgcttttcg
     1021 ggatcgtcat cacgggtgtc tcctgctatg agggcttcac caccacacag ggcgcggtcg
     1081 gtgtgggaca ggccacgcgg cgttcggtga tctcctcctt tctgctgatc ctaatactcg
     1141 ggtacatcgt cacccgcatg ttttacgacc tatgatccgc tttgaccatg tcaccaaaca
     1201 gttggggggc cgtctggtgc tcgacgacct ttctttcgaa atcccgaagg gggagatctt
     1261 cgcgatcgtc ggtccctcgg gcaccgggaa gagcgtcaca ctcaaacaca tggtgcgcct
     1321 gatgaccccc acacgcggtg cggtgtggat cgacgggacg gaaatcagtg ccgtggacgg
     1381 ggcggatttg gcgcgcgcgc gagaacgctt cggctacctg ttccaaggcg gcgcgctgtt
     1441 gggctggatg accgtcgagg agaacatcgc cctgccgttg cgtgaaaaga cgcgcctttc
     1501 cgaagcggag atcgcggagc gggtggccaa ggccttgtgc gcggtcgggt tggaggagga
     1561 cggcgacaaa tttcccagtg agatttccgg cgggatgcag aagcgtgccg ggttggcgcg
     1621 ggccatcgtg cgtgaaccgg agatcgtgct ctatgatgag cccacttccg gtctcgatcc
     1681 ggtgaccgcg cgcacgatcg acaaactgat ccggcggctc aacaaggagc tgggcatcac
     1741 cagcgtggtg gtgacgcatg atttgcaggg tgccttgttg agtgcggacc gcatcgccat
     1801 gctgacgggc gggcggtttg tggaggtcag ttcgccgcac gcgtttgtgg ccgcggacca
     1861 gccggatgtc cggcgttttc tggacgccca gtttatttca aaaaaaggga ttgaggggat
     1921 gtgaacgcgc gggtccgtca cgcacggtcc gtaaccgcaa aaggaggaac gtatggcttt
     1981 gaaacgcaac cgcgaggtct tttccgaggt gattgtcggc ctgtttatgg tcgcggtcct
     2041 cggactgctg gcctatttca ccatcatcat ctccggtatc gatatcctga acggcaagaa
     2101 caaggggccg gtcacggtgc tgtttaccga tgtcggcgga ttgaaggtgc gcgacagcgt
     2161 gattctgcgc gggatgacgg tggggtccgt gagcgagatg cacttggaga atagcggggt
     2221 gcgggtcacg ttgctgatcg atggcggggt gcgcttgaag gaggggtacc gcatctcggt
     2281 caacaccgga tctttgctgg gcggcaacta cctgctgatc gaagaagggg agggtgccga
     2341 agtgccgcgg gaaaccgtgt tgcggggtga gacgccttcc cagtggatgc gcgatctggg
     2401 ggtaattgtg gccaatctgc gcgaggccac cgaaggcagc cacctccgca acaccctcac
     2461 caatctggac gttgccgtgg cctcgatccg cgcggcggtc acgcgtgtcg aagagggccg
     2521 gggtacgctc ggcaagctgt tttccgatga catgacgctc tacaacgacc tctccaacac
     2581 tgttgcgagc ctgcgttcgg tggcggtcaa aatcgatgcc ggcgagaact cgctaggccg
     2641 gctgatcaac gacgacggca acgtctacac caacctcagc gcctcgatcg cgaacctcaa
     2701 gtcgatttcc gaccggctgg aaaaggggga gggcacactc ggcaaactgc tctcttcgga
     2761 tgccggaata tatgacgacc tgcgcgagac ggttgagaac ttgaagacgg tttcggcgcg
     2821 tttggagaag ggggacggca cgctcggcaa gttgctgaaa gacgacaaca aagtctacaa
     2881 cgacctcgac gcgacagtgg cgaatctgcg cgaggtgacc gatcggctcg tccggggcga
     2941 aggcacgctc ggcaaacttt ccaaagagga tgaactgtac tccgacacgc agggactcat
     3001 ccgggatgtg cgacaaaccg ttgataactt tcgcgacacg accccgatca gcgcattcgc
     3061 cagtctgatc atgggcggat tataaaccgg agaaccccgt atgctgtaca cacttggaga
     3121 aatggcggag aagttgaacc gatcccccgt ggtattgcga ggtcttcagc gtagtttcaa
     3181 gctgcctgtg taccggggcg cggattattc cgaatcctat ctggccttct tggatacggt
     3241 gcttgccttg cgcaccctga acgttgcgga gaagacactg ctgaaactct ggcgcatcga
     3301 gcgaaaactc atgcgcatgc tgcatgccga caccaccgga tcgcccactt ggtttctcga
     3361 ttcctgcggg tcgacgcgca accgcaagcg gcggttgctg ctcagtcatt ttgatatcgg
     3421 aaccaccctg ccctccggtg ccatccaact cggcttcgcc ttttcggaac cgcagcgcga
     3481 acggttcgct agtcggggaa tgggggagga tgttctcgcc gtgcttaacc tctacaccgg
     3541 gttgcacaat gagatcagcg ataccgtccg agcggaaatg ccccgcctgt tcagcgcgct
     3601 ttccttgtcc aaatggttcg gcaggtaggg tggccccatn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnng tccgcggacg cctcggggag gcgtccctgc caacgggcgt
     3781 gggcctcgtc tgttctgcgc tgtcgtgctc catcaaagtt atcctcattt acattcccgt
     3841 gcgaagcgcg ggcggcgggc taacttctgt ccacttctcc cttcgtgagt cttgctttaa
     3901 cgcagcattt gagttatata atagcgggaa agcgaaacga agttagttcg gaggttccca
     3961 atgcatgtca atacccaagt ctccgctttc atggatcagt tcccgtgtga agggctgacc
     4021 tttgacgatg tcacgctcgt gacccggtac gccgattttc tcccggacga gaccaacctc
     4081 gcctcgcccc tcaccagtcg catcacgctg aacatcccgt tcgtgagtgc ggccatggac
     4141 acggtcacgg agttcggcat ggccacggcg atggctatgc tcggcggcat cggtgtgatc
     4201 cacaaaaatc ttccgcccga acttcaggcg cagcatgtcc ggcgagtgaa gcagtatctc
     4261 aacggtctca tcatgcggcc ggtcgtgttc cacactcagg atacccttgc gcacatccag
     4321 gcctatcgga atgagcaccg cctgtctttc agcggtttcc cgattctgga cgatcaagac
     4381 cgtctcgcgg gaatcatcac ttccagcgat atccggttcg ccaaaaaaag cgcgtcgtgc
     4441 gtgaccgagg tgatgacgac acgggtcatc accgccgctc ccggaacttc gctggaagag
     4501 gcgtatgcca tcatgatgga gaacaagatc ggcaaactgc cgctggtgga tggcggcggc
     4561 cgcctggtcg ggctctatgc attcaccgat gtgcaggagt tggttgagaa cgcccagcct
     4621 ctttataccc gcgacagccg ttaccgtctg cgtgtggcgg cggccgtcag cgcgggggat
     4681 catgagcgca tgggcttgct cgcggaggag gatgtcgatg tcgtggtggt ggacagcgcc
     4741 cacggccatt cgaaggggat tctcgatatg gtgacgtgga tcacgaagca ctatccggac
     4801 atcgacgtga tcggcgggaa cgtggccacg ggcgaaggcg ccctcgcgtt gttgaaggcc
     4861 ggtgcccacg gcgtgaaggt cgggatcgga cccggttcat ttgcaccacc cgcgtggtgt
     4921 gcggcgtggg catcccgcag atcaccgccg tgtaccacgc ggcacgggct ctcggtggcg
     4981 aaattccggt catcgccgac ggcggtgtcc gccattcggg ggacgtcccc aaggcattgg
     5041 tcgcgggggc ggacaccgtg atgctcggtt cgatcctcgc gggcacggag gagagccccg
     5101 gagagaaaat catccacgag ggacgccatt atgtggtgta ccgcggcatg gggagcctgg
     5161 ccgcgatgaa agagggcatg ggcagtcgcc agcgttacgg gcaggacaat gtccagaccg
     5221 atgatctggt gccgcagggc atcgagggta ttgtgccgct ggtcggctcg gtcaagaaaa
     5281 ccatgaccca gttctgcggt ggcttgcgct cctccctcgg gtactgcggt tcgcggaatt
     5341 tggcggaact caaggccaac ggctgtttct tccgcgtctc caatgccggt gtgcgcgagg
     5401 cccatcccca cgacgtcaag gtcatcaaag aagcgcccaa ctaccgctcc tgatccggat
     5461 ctcggatcgt acggcaatgc gggaaaaatc acgcgagttc gatgtgttcg gcgatcgggc
     5521 gcgagacgag gaagcgggcg tcgcccgcgc cgaccaccac ggaatgttcg gatttgcggt
     5581 tgcggaacat ggtgacgcgc acgcccgcaa aaagtcccat ctccgcggat ttacggtcgc
     5641 gattactgcg gatgcgcccg gtttccccgt cctggaggtc cagcaggttc cgcgtttttc
     5701 ccgctgtggc gggggttccg gtttcagact tcatgtgcga tccgtttccc ataagcggcc
     5761 agtagggcga ttccggctgc ggtgagggcc gtcacaaacg ccaaccagcc tacggaccgc
     5821 aagggtgcgg cggggaagga ggcgcattga taaaaaacgg tggcggtcaa ccaagccagt
     5881 aaggattggt aggtgacggc aaaccccatc caacgccagc ccgcctcgcg tgcgagcacg
     5941 atcagggccg cgacacaggg cgaatagatc aggacaaaga gcaagtaggc gtatgccgcg
     6001 gtcggggagg cgaagttccg gcgtaacgtg gaaaggagtc cctgccgggc ggccgggtcg
     6061 gcggaacggt ccgggtctgc ggtggccgca gacccggaga atccggcgaa cagcgcgctt
     6121 cccgcagcgc ggagttccgc gcgcagatca aagggcttcc gcgaatccgg agccgtcttt
     6181 tccgtttgaa aataaagcac ctcaagcgtc cccacaatcg cctctttggc catcaggcct
     6241 gtgagcaggg ccgctgccgc gggccagttg tcccccgtca cacccatagg acggagcggg
     6301 acggttagaa cccggcccgc cgcactccgg tgcgcaaaag ccgtttccgg gttacggtac
     6361 gctttttcaa aaagggcatt ggccagactg agcagaatcg cgatcgtcaa aatgacctga
     6421 cccgcccgga ccagaaagct ttggagattg gcccagacat gctccagaca tccgcgcaag
     6481 gccggcaggt ggtagggcgg cagctccatg acgaacgctg aggcggctcc gccgcggaag
     6541 agggtgcggt gcagcatcag accggagaga atggccaata ctacgccggt gaggtagagc
     6601 gaaaaaatga cgatgttgcc ctgattcggg aaaaaggcca tggcgaaaat cgcatagacc
     6661 ggcatgcgcg cgccgcagga catgaatggg gcgatcagca tggtcaggag cctgtcgcga
     6721 cgttgttcca gcgtgcgggt ggccagcagg gccggcacgg tgcagccgaa gcccacgagc
     6781 agggggatga aggccttgcc gggcagtccg atacggttca ggaagcggtc catgatgaac
     6841 gcggcacgcg ccatatagcc ggactcctcc agcaggctca ggcagagaaa gatcatgaag
     6901 atcgggggca tgaacgtggc aaccgtcgta aggcccgcgc cgaccccgtc acagagcaac
     6961 gagcccagga tctcgggcat gcccagacgg gccagccaga tgcgcggagc gttgacgagc
     7021 aaggcgttca acgccagatc cgtgaaatcg accagcggtt gggtcagccg cacggtcaac
     7081 cagaaaacca cgtacatcac gccgaggaaa aacgggatgc ccagcggacg gctgagcaac
     7141 agccggtccg ccacatccga gacgcggcgt cgcgagactc gcttacgctg aaccacgtcc
     7201 cgcgccaagc cgcggatgaa tccgaggcgc gcgtccatca ccgcctcttc gggtttctga
     7261 ccggtgtggc gcgcgatacg ggcgcactct tcttcggcga gttcgtcgaa cagccctgcg
     7321 gtcagttcgc gggcaaaggg atcgcgttcc agcagtttga tggccagcca gcgcgagtcc
     7381 acacccgcat catcggctgc cacgatcagg cagacctgca aacggcgcac cgacggttcc
     7441 gcgtcgccgt attgaacgcg ggtggtgggc aggctgcgcg tctccgccac atggcagatc
     7501 gcctgcttga gggcttcgag ccccacgcct ttagaggcga ccatcggaat caccgggcac
     7561 ccgagatggc tggcgagatg atcggcgtct acgtggatcc cgtagcgttc ggccacgtcg
     7621 gtctggttga gggcaaccac cagcggcagg cgcatatcga gcaactgggc ggtcaagtag
     7681 agactacgct caaggttgac ggcatccacc agattgacga tcacggcagg cttctgctgg
     7741 atgaggaaat cccgggccac acgctcgtcc tgcgtgtgcg gagaaaaaga gtaaatgccc
     7801 ggcaggtcgg tgacgcgggc cgttttgcca ttcagggtaa aggcggcgtg cgcggtatcc
     7861 accgtcactc cggtccaatt gccggtgcgt tcgtgcgctc cggtcagcaa gttgaacagg
     7921 gtggtcttgc cggtattggg gttccccgcc aacgcaatgg tcagatcgtc acgcatgcgg
     7981 cacgttccgg ttccaattgt tagccaggga aaacatgggc cgaattatat ggggcggaac
     8041 ccccgagcac aagcgtaatc gctggatttt tgttgttgac ggagggaagg tgcggtgcga
     8101 tggtataaac aaacgcccta cagggtgtgt gccctcccaa aggagtcaga atctcatgtc
     8161 ccaaccggtt cggatcatgc cgtgtctcga tatgcgggac ggtcgcgttg ttaaaggcgt
     8221 gcgttttgtg gatatccggg atgccggcga tccggtcgcg tgcgcgcggg cctattgcga
     8281 aaagggggcc gacgagctgg cgatgctgga catcacggca accgtggagg ggcgggccac
     8341 gctgctgcag gtggtccgta acgtggcgga ggccgtgacg gtgccgttta cggtgggggg
     8401 cgggatttcg gatgtaaaga ccgcgcgggc ggtgatggaa gcgggggcaa acaaggtttc
     8461 cgtcagtagc gcggcgttcc gcaagccggc gcttatcccg gagttggtcg ccgaactggg
     8521 tgcggagcgg gtgaccgtgg ccattgatgt ggaccggaat gcggcgctgc cctcgggata
     8581 tgaggtgtac atcgacggcg ggcgtaccgc gaccggtcac gatgcggtcg aatgggccaa
     8641 gcgggtggac gggttagggg tgcgggtgat tctgcccacc agcaaagcgg gggacggggc
     8701 gcgcacaggg tatgatctgc cggtgatccg cgcaatccgg caggcggttt cggcggaggt
     8761 ggtggcctcg ggcggcgccg gaacgttgga gcatttccac gaggcggttg aggccggcgc
     8821 cacgattctg ctggccgctt cgatcttcca tttcgggatc atcggtattc cggaactcaa
     8881 agcgtatctg cgggaacgcg gggtcaaggt ctgcggcggc gaagcttgac gcgcgtgcca
     8941 cgcggtcggg gaggtgtatg atgaaagtgg tcgcatttaa cggaagcccc cgtcaggacg
     9001 ggaacacggc gctgctgctg cgcaaggtgc tggcaccggt cgcccgctcg ggaatcgaca
     9061 ccgagttggt gcaggtcggg gggcacgcgg tccacggctg taccgcctgc atgcgctgca
     9121 tgaccgcccg caaccggcag tgcgcgatca cgaccgacag ggtgaacacc tggatcgcaa
     9181 agatgattga ggcggacggg atcgtcatgg ggtcgccttc ctacttctct ggcatgacgc
     9241 ctgaactcaa agcgctgctg gaccgggccg ggctggtggc ggtggccaac gggcggatgt
     9301 tcgcgcgcaa aatcggcgcg gccgtggcgg cacaccgccg cggcggcgcg gtgaatgtgg
     9361 tggacgccat caaccatctg ttcctgatgt cacgcatgat cgttccggga tccacgtact
     9421 ggaatttcgg cgtgggcttg gagcgtgagg atgtggcgga cgacgcggaa gcgatggaga
     9481 acatgcacga tctcggggag accctcgtct ggttgctcaa gcggcttcac gcggacacct
     9541 gaagcggggc gcgtgctcag aaaccaaccc caaataaagc cagatcaacg gacgggacgc
     9601 ggctcttttg tgcgaggttc gagactttcg cggagtgcgg cgagatcgcg ttgcagttgg
     9661 acaagttttt cgtccagggc ggagattcgc ttgtcgagcg tggggtaggc ggccagaacc
     9721 gcttcgttcg ccggatcata gaccagcccc agaaaggcgt agagcggggt gaaatagctg
     9781 tcgatgtccg caagccgttt ttccattgcc gccagacgtt cggcgaaatc ctcgggcacg
     9841 gccgtgacgg tgaccgtctc ctccagatgc tcgaacgctt gatcgagata ggcggtgagt
     9901 tccgtcagca gatcgggcag ggcgttcagg tgtccaatcg cggcgcggaa ttcgtccgtg
     9961 cggagtgcgg cggggctgtg tggtgccgcg gaaccggtgg gggcggcgcg cgtctccttc
    10021 ggtgcccggg attcgaggtg tgacaggcgg cggttcagtg tggcgagctg gacattcagg
    10081 gtttccgcct tccgcaacag gtcagcctgg cgctcccgta acccatcggg ttccgcaaga
    10141 gcctgatgaa acacactgcg gatccggcgg ccttcttcgc ggattgcgcg ttggaacgcc
    10201 atgactgcgg ccagcagcgc cagaccgagg gccagaaagg caaccgtctt tctcatgcgc
    10261 acctccggga gtcagggcgt tccgtctcgg ttctttacgg tgccgaccgc gcgacggagt
    10321 ctcaagcccg ccagggacgc tcgcgttcct gacaggaggg gaagatttcg ttgaagagat
    10381 tcacgttgtc gaccaactgg aaatcgtaca tgccgacgca caggaaatcg gcaccagcct
    10441 tgagcgcgaa cgggaacccg tctttaggcc ggatcgcgcc ggccgccagg gtcttgaacc
    10501 cgatccacgg ttccggccgc tctttcatgt aggcgatggt ctctttcggg tttgtgcacc
    10561 agatgttgtc gtggcgcgtg ccgccgttga tgttcgctga ccagtagttg atcggatgga
    10621 tggttttgac ccagtagtcg ggaatcagac cgcggtcgac gcactctttg accgcctcca
    10681 gactgtgggc gccgataccc gcctgatagc cttctttgcg catgaaggcg agggcgtctt
    10741 ccatgatctt gtagttcttt tcccgaatga tgtatttttc ggtcatctcc ccttggatgt
    10801 acaacatctg cgcgccgaat ttgatcgaaa tctccgctcc tttgatggga ttgccgccgg
    10861 ccgcgcaatc ggagagcacc tgcattttgc cgccctcttt cttcagataa tcccccatca
    10921 cgcggaagag tccgggattc aggatgatgg tgttcatgcc ggctgcttcc gccatgtgga
    10981 gcgtgcggaa gacgcgccag tcctcgtggt aggccttgac gagttggctg acgtagatca
    11041 ggtcgcgcgc atgcgcccag ccgccgatca ggttacaccc catgaacatg cggctgagcc
    11101 gggtcgagcc gatctgcccg aaggcttcga tcggcttctt gagtttgctg atgtctttgg
    11161 attcaaaggt gcggagcgtg ggaccggtca tggcgtccgc gatgcttttc agatgctctt
    11221 tctcccatac cgggccgtgc ttctctatga aggcgcctgc aaaggctccc agaacgggca
    11281 gggaactgag gttcttgagg aattcgcgcc ggtcgaatgc gatcgggctt tcgcgtcgat
    11341 tccggtactg ctggaacagg gcgcccaacc cgtaagaggg gatcacgcaa atcaccgcca
    11401 gcgcgaaggc ttcgatgaca ttccggtcga tccacagaag atgggattcg ccgcctgcgg
    11461 gcgcggtgaa gggaggatgg gccagatagt acaacgccag gaggacgatg ccgctaatgg
    11521 atgcgaaccg gatggcgact cccaaaatga gggcgaggcc gatcagggtc aatccccaga
    11581 tgttcaggag gtcgacgacc ttcagcagcg ccggcgtttc cgcgatcgta tgaaacagtt
    11641 tcgcggcgat ccattcgctg gaactcaggt aaggttcggc gctccagctt ccggagagca
    11701 acttccagag gccctcatat aagaaatgcc agccgaccag aaaccgtagc aaggtgaccc
    11761 agtaggattt tttcatgttt tccctttctg tctgtctttc cgttcgttaa aaaaccaccg
    11821 ttgcgctgac cgggaaatga tcggaggggt agttcccgtt ttccgcgtcg ttgtgcgtgg
    11881 tgacgtcccg caccgtcacc ttttcagaaa cgaagatgta atcgatgcgc ggcgcatctt
    11941 tggcgggggc cttcggctct tcggcgtcag gccggtaccg gtacccggta aaagtgcgcc
    12001 acggaccttg atgcggggtt tgcgtgtggc ggacggcatc ccgcaacacc tttccggcca
    12061 actggaccgg ttcggaggtt tcgttgcagt tcatgtcgcc ggtcaggaag atcggccatc
    12121 cttgcggcac gagcgtctgc atacgctcca gaatcagttt cattccgttg gcgcgggctt
    12181 ccacgctgat atgatccaga tgggtgttga agaacgcgaa cggtttgttg tttttcgtgt
    12241 cggtcatcag ggcccaggtg cagacacgcg tgcaggcggt tttccaggac ttcgaccccg
    12301 gcacctcggg cgtctcggag agccagaagg ttccgctctt ctgaaccgtg aaccggtcag
    12361 cccggtaaag gatgcaagtg gcttcgcctt tacgcttccc gtcatcacgg cagacgccga
    12421 ccatggccca gccgggaagc gcggcaagca tttcgtcgaa ctgcatagcg gttgcctctt
    12481 gcaaacccac cagatcgaat ccgtgccgct tgaccagcgc gatgacgcgg ggggcgcggt
    12541 tgctccaagc gttgacaccc ttatccgccg gaagccggat gttgaaggtc gcgagacgca
    12601 acggctccgc aaccgcggaa tggcatgtca tgaacccggc caacaggcac aagtataaag
    12661 tgcggatcct catcgcaagt tccccctgat cgtgatcgcg taatgtcaca tcatgccaca
    12721 aacgctaccg acctgtcaaa aaagcaatcg agcgaatcac aacaacagcg cggcaagaag
    12781 gcggacattc tcgtctgagg atgcggcggg cagccgctcc gacgctgtgg cgcggtctcc
    12841 ctcaaaaacg gcgcggcacc agacgccgac ggcatcggtg ccaaaggccg cggccacggc
    12901 gtccgccttc gcgctctgcc cgcgttcgcg gcacgccagc gcggtcagca ggtccagcgc
    12961 atttgcggtg cgcgccggac gactgggggc gaatcccgcc gcgacccggt cgagaaggtc
    13021 tgcgcgggtg acgccctgtt caagttgcgc gaagagccag agttcgaggc gcatgtcgat
    13081 ctcgctcgca tagggcttgc cgacgccgag tttctcgtcc cagatgccgg cgcgccggac
    13141 cgcatcccgc gtaacggcgc tgtcgctctt gcggaacgcg gcgaccgcct gcgcgaggca
    13201 ggcttcgcga tacaccctgc gggtgatcga ggtgaactct gacggcagga accgtacggt
    13261 gtcgagggtt tcgagacagc gggcgggatc acccagccgc agatgggact gggccagcag
    13321 aatgcctgca tcgttgtctt cggggtgggc ggccaacaac cgttgcgcgg cacccagagc
    13381 ctgccgccag tcattggctt cggcatgggc gcgccattcc gcttttacag cgtaccggtt
    13441 ctcgccgaac gtctgggcct tccggatgtc ggccagcgcg gacggcccgt cacgcaggcg
    13501 cgcgcggaaa agcaggaacg gcacgtcatc ggcatcggtc tcgttcaaga gggcccgtgc
    13561 ccggtcgtcg ttaccgaaat ggcgcaggag cagggcgagc gcgtaacgcg gtttccaaga
    13621 gggggaatgg gcgtgcgccc attcaaggat cgggagggtc tccggccgga agggaaaggc
    13681 aaaggcggta tccagccgct ccgcgtcggc cagcgcgttc cgggcggcgg tttcatcgcc
    13741 ggtccgcgcc agccaatacg cgcgcgccac ggcagcgagg aagcgtccgg acgcgagcgc
    13801 ggcgatctcc gcggcctcgc cgaacagacc ggcggcggcg tacaggtgcg cggtttcgag
    13861 gatctcctcg tgcggcaatt cgcaacggat ccccgcagca aaggcggcac ggtcctgttc
    13921 gcaacgcgcc ggatggaaga gcggaaaacg gtcgagcacc gcagcgcagg ctgcccggtg
    13981 ttcatcctcc cgccccaaca ggcggagggc tacgaggcgc aggtgcagcg cggaaaactc
    14041 caaggggttc gcttccagtg cgcgctcggc cgcctccagc atccggtcgg gcctcccggc
    14101 ggcacactcc agcgcggcga tccgcgtgat ggcggcgctg cggaaaagcg gcgacagcgc
    14161 ggccgcgccg aaccgttcga gggcgttcgc gtgatccccg cgctccgcga agacgattcc
    14221 gctgaggtgg ttcgccttgt cgtgataggc gtcaacggcc agcgcacgct cgctgaaggc
    14281 gagggcttcg gcgggacggc cgcagtggag cagcaggtcg gccatgcaga ccagcgcggg
    14341 gaggtaatgc ggttctttcg ccagcacggc gcgcaacgtt ttctccgccg cagtgtagtc
    14401 cttgaggttc acatcctgtt ccgcctgaag cgtctggccg taaaccgtgc cccagtcgaa
    14461 gtcggcgggc gtcgcgagcg ggcggctgac cgtaccggat tccgcggcgc gctcctccag
    14521 aatccgggcc tcggttaccg gtgaccaacg ctcgctgaag gtgatcgtcg aaccggcggg
    14581 gaaggagggg tatttgaacg gggtgcggtg actgccatgg gacggctggt tgaaatagcg
    14641 gcccgactgc agctcgacat attggccgtc ctcatcggtg aggagatcct cccagatggc
    14701 accggaacgc gacaggctcc aaatccagat tttgcgtcca agccggtcga agcgggcagc
    14761 ctcatgcatg gaaccgaact ggtgcgcgtc ccaccagacg ccgaagaacc ccgtgtcgcc
    14821 gttgacgaca tgatagctct tcgacaggcc gaaggcgttg ttcgtataaa cggaaagatc
    14881 cagtccggaa gcgtcccgcg gccacggtga ggctgtcccg tcatgttcga gccgggccgt
    14941 acccggaaag acatatcggc cgccacacgg ataggcggcg ttcatccact ggtaacaggg
    15001 ttgcggcagg ccggaggcgt tgtgccaggt ggtctgcgtg gtgaaaaagc ggtcggaggc
    15061 gccgagacgg atctcgacgc accagcgggt gcgcgtgatg agttccagca caccgacaaa
    15121 acaacttgcc gaaccgtcgg agttcgtgcg gcaggcgtaa tccacggggg tcgaggcggt
    15181 cggcgcatgt ccgagcatgc cgaagttgaa ctcgatgccc ccgctggtcc agggtccccg
    15241 cgtggagatg ttgcggaact tgacgacgcg gttacgatag aggaagtccc gtccggtccg
    15301 cttgtcccgc gcgctccaga ccttcccgcc gatttgcgga aacacatcca cctcgaccgc
    15361 gtcgttttcc agccggacgc aggtccagga ttgcgttgtg gcaaccgccg tgtacccctc
    15421 gaatttgaag tacggccaga tctggcggtc cactctggtt tcgggttccg gaatcgggtt
    15481 cggtgccgag aacccgtaag tgggcatctc cagcgtgatt tccttgacgc gtgcggcacc
    15541 atgcgaaaag caagggaagg ccacgcccgt gaaaccggtc agcagaaaaa gcgttgttgc
    15601 cgtcagccgg gcgggttgtg atatcgcgcc ccttcgttta tcctgtacat tcatggcgaa
    15661 tcctttgtta tatgaacagt ctatcagagc gacggccggt ttttcaatta tgggatgttt
    15721 gaaaaaagac aggctacgca attaaacgcg ggattccacc gtctgcggta tggtatgatc
    15781 cgaaatacgg gagaagaaat acgggagaag aggccatgcg taatgccggg ctggcaggat
    15841 ggatcatgtt gtgcggcatg gtgtcgggat atccggctct cgctgcgggc cgggtgattc
    15901 tcagaccggt tgccgcaccg ccgccggttg aagtggccgc gcgcgggttc cagaaggatc
    15961 acgaggccgt aaccggggtg cggcttgaga ttatgacggc gccggatgcc gcgaccgatg
    16021 tttaaacaag gggaaggaac agagatgccg gatcaggttg atgttacgct gacggaacgg
    16081 attaaacaat tcgcgctgga gggactgaac gcggatctgg tgggggtggc gaacatcggg
    16141 cgcttccacg gcgcgccgac gatgatgagc cctcagggta tcctgccttc ggcgcgctcg
    16201 gtgatcgtga tggctgtgca tcatcccgat gcggcgatcg agttgggggg ggaggagcat
    16261 ccgcagcaga ttgggccgta ccgcatccag tacgcgatga acatgcgcct cgacgagatg
    16321 agctaccgca tggggcggtt catcgagtcg ctgggccaca cggcggtgcc gatcgtctcc
    16381 tctaacatct ggcgctacaa agggtacaag gagttgcggg agcacttcgc gccggacatg
    16441 tcgcacctgc atgcggccgt ggcggccggg ctttcggagt tcggctattc ggggttgtcg
    16501 atcacgccgg agttcggtgc gcgtcagcgg tatgtctcgg tcatcaccga cgccgcgctc
    16561 acgccttcgc cgctggtcga gccgggctcg gtctgcgaca tgtgcatgct gtgcaaaaag
    16621 gtatgcccga ccgccgcgct gacccgggaa ctcgacggct ggaatgaggt ggagatagag
    16681 ggcaaggtct accggtatgt gaggaaaaac ctgtggcggt gcgcgtgggg cgaacatttt
    16741 gacctcgatc tggatttgga gataccggaa aaggtggacg aggcggtcat cctcgaccac
    16801 gtcaagcgtt tcgggcggcg cggcggcgag atggggtgct gcctcaaggt gtgcgtgccc
    16861 aagatgcgcc gcgtgctcga ccggacgtac tgcacaggac cgcgtcgcag acgcgacgtg
    16921 atgccgccgc cgggacctgt gccgcgcgag gtgaccgagc ggcttcatgc gctgacccgt
    16981 gcgcatggcg tgtcgtgtat cctgactgtg ccggttgaca cgttgcgtgc cgcaggggtt
    17041 acggtcgagg attccaaggc atatctgccg ggcgtgaaaa gcgcggtgat cgcctgcctc
    17101 gccgcagaca gtgcactggt acgggaaacg accggcacgg acaaagaact ggcgctcgag
    17161 atcacgctgg tttcgggagg cctgcgcgaa accgccgaga agctggtcct gcagaccggt
    17221 tacgatctcg cccgagaact ggaacgggct ggttacacgg cgaccggtgg caccgaactc
    17281 agcgagagtg atgtggcccg agccgtctcg ggcgtgccgg aaggacagac gcttttcacc
    17341 tataccgtgc tgaccgatgt ggaattgccc tctacgggct tggcattccg cgcgccgccc
    17401 gtggagagtg cggaggatct cacggaggcg ctcaaagccg aggccgggcg tctgggggcg
    17461 gacgtgtgcg gtgtcacgtc cattggccgg ctgcagggcg tggcccaaca ggtgcggcgc
    17521 gtgttcgaag gggaggaggt gttggacgcg caggatacct cgcagctctt cacgccgtac
    17581 cggccggtca tcacgtcgcg ccgacggcag atccccgatc cggcgacgct gttgcccggc
    17641 agtcggtcgg tgttcgtctt cggtatcgcg ttgcctcacg gctcggtgga catcacggca
    17701 cggacaccgg ccgaggctgt ggggccgtac gcgttcgcgt tgtacgagaa cctgaactac
    17761 ctgcgccgcg tcgctcttcg gctcgccttg tggttgtcgc ggcagggcgt caaagcgttc
    17821 gtcagctcgg atctgctggg cacgggttcg atgaccgcca gtccgcgcgg tgcggttccc
    17881 gacctgttct caaaccggtt tgaggcttgg gccgcggggc tgggacgctt cgcgaaagcg
    17941 gggttcctcg tgaatccgcg gtttggcacc aacgtccgtt acatggccgt ggtggtggat
    18001 cgcgaactga gcgaggatgc cctgcaaaac ggcgctgctc ccgaaacctg tgccgcgtgc
    18061 ggcgaacgct gcgtggcggc gtgcgcgtcc cgggcgttcg gagaaaccat gaccgtggag
    18121 gcggagggcg tgcgcgacac gttccgcacg attcagacgg tgcggtgtga ctgggcgaag
    18181 cggtactctc tgatcgcgga tgaagggtgc gggttcgtgg ggtggtccct gaatctgccg
    18241 ccgccggaga cgatcacgcc cgaggcgctg gcggaggggt tgcggcaaca gccgcc
//
LOCUS       DAOSRB010000038        17894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3281_length_17894_cov_7.376086, whole genome shotgun sequence.
ACCESSION   DAOSRB010000038 DAOSRB010000000
VERSION     DAOSRB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17894
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3281_length_17894_cov_7.376086"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..2439
                     /locus_tag="PLZ74_03560"
     CDS             217..2439
                     /locus_tag="PLZ74_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97431.1"
                     /translation="MREPGRFGWMVGLMAAMAVAGASAQTVFHVDAQRGQDTQDGSAA
                     RPFASLKRAQTAIRELKTNQTFPEKGVTVEVSGTFVMPEETVSFEESDSGIGPNAPVI
                     VRAAKAGALFLGGRRLPAEGFKPVSDAATLARLPEAVRGKVVAFDLKTIGVTRLAPLP
                     DKFHGWREMELFSNGKAMRLARWPNTGWAQIAKVIDRGVKPIDRATGEWEFGVRGGIF
                     EYAEDAPARWDVSKGIWMNGFWCHDWANETLRIGAIDKEKKQITSAAIHTYGIGNSSK
                     WHTAKRRYYVFNLLEELDMPGEWYVDREACLLYLYPTDSGLGDVVLTDQKKPLFRLNK
                     VRYVRLDGLTFRYNTGLAVSVNGCEEVRLENLRVSDITLDGIHITGGRACGLNRCEVY
                     GVGGTCVSVSGGDRKSLTACDHFVTNCRLHHSGRLQRTQGKCLNFNGVGIRIAQNLIY
                     DAPYIAVTYGGNENLFEYNEVHSAMMESGDGGGLYTGRNWCSQGNVIRHNYLHHFGQP
                     GVDWQKARGLNPDYEPLKESVMVMGVYLDDCDSGDTICDNIFYRAGWAAFVGGGRYNT
                     VRNNLFIECTSALHLDNRGLKRARPGEGTKDGWDLLAKLQAVKWQESPWKDKYPHLVN
                     IMEDEPKLPLHNVFRENVAVNCKLFLNANNVVRTGVVHRLTFENNLSFGPIDKRDVMA
                     FPQTDEGKRRVTFLETPLPDSVDPDTNGFRIQDSGEFKRLAPWFKRISLERIGPEKKN
                     "
     gene            2521..4053
                     /locus_tag="PLZ74_03565"
     CDS             2521..4053
                     /locus_tag="PLZ74_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOR97432.1"
                     /translation="MKKMRKLGWVGAILLAGSVQAAVRMPKIFGDGMVLQQQVAVPVW
                     GWAAPGERVTVTFAGQSKETAAKADGTWSLKLDALQASSEPARLEVRGANALTFSDVV
                     VGEVWFCSGQSNMEWTMRSVDHAEKEIAAANLPLIRHFKAPKRVSGLPLDDVNAVWEK
                     TSPAGIGDESAVAFLFGRRLHQVLNVPIGLINCSWGGTRIEPWTPLCGFEGIPALEGI
                     LRNAQKRVEQVSPGSTSYKATHQTYFTRLQEWIAESEKRLAANQAVTAMPEYPSVFSF
                     RNQDPTVLFNGMVAGLVPYAIRGAIWYQGCSNNGEGMLYLEKTKALVKGWRQVWGQGD
                     FPYYLVQLAPYTYGGNPENLPGIWEAQAAVPAAIPNTGYTVINDIGNIRDIHPRNKQD
                     VGLRLANQALNRTYGRSEIAWAGPVYRSFAIEGQTVRVTFDHAEGLKTRDGQPPTWFE
                     ICGEDGRFVKADAKIDGTSVILSASSVSAPKAMRFAWNQIAEPNLVNGIGLPAGAFRC
                     GK"
     gene            complement(4103..4678)
                     /gene="pyrE"
                     /locus_tag="PLZ74_03570"
     CDS             complement(4103..4678)
                     /gene="pyrE"
                     /locus_tag="PLZ74_03570"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOR97433.1"
                     /translation="MTDKEILKTLEETGALLSGHFELRSGLHSNRFFQCANVLRYPRV
                     AGRLCEELTARARAAKALDGGVDGVISPALGGILVGHEVARALDVKCVFAEKQDGALV
                     MRRFTLKPGERYVVAEDVITRGGRVQETIDLVKAADAEVVAVLVMVDRSAGQAVFPYP
                     TFSLLHMAPEVWEPAACPLCARNEPFVHPGS"
     gene            complement(4675..5364)
                     /locus_tag="PLZ74_03575"
     CDS             complement(4675..5364)
                     /locus_tag="PLZ74_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR97434.1"
                     /translation="MSNGADVLVTGVVRTFRPPGGVPVEVLRGADLTVTAGGSVAVTG
                     PSGSGKTTLLHLIGALDTPDAGEIRVNGQRLDALEEAARLRYRNREVGFVFQNHHLLP
                     QLTALENVLVPAWSGPDATARERAEHLLERVGLGDRMRHFPGHLSGGERQRVAVARAL
                     VMDPGLVLADEPTGALDQKNAAALIDLLLELNAERRATLIVVTHSELCARRMARRVKL
                     ENGTVREETGP"
     gene            complement(5357..6514)
                     /locus_tag="PLZ74_03580"
     CDS             complement(5357..6514)
                     /locus_tag="PLZ74_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOR97435.1"
                     /translation="MMSRRPIPSRRPATAYVAVAAVIAAVAVIGWLARSMCAPQPVPS
                     AAQPAVRNAWRVVWRAALTAHANAPVPVDAEGWIVTDDAGGVTRLSDTGKPLWRVSFS
                     NQPFEASAAIHDRRVFAASTSGGVLALDAETGRALWSVTLDARFQHPPLLDAQGGGDA
                     AVMRLVSQSDGRLFCLRVRDGLTLWQSEPTNRCDGEPAAGPYGIAYGNCDGAIHVFDA
                     ADGRRKGSIPVGTEDQMAGGLLSLPDSRLVAGTRQGNLVVANARTFVCEARTRVSENE
                     AFLKPVRTQNGLIATGSEEGLVSLWFLRDTTLSQAGEYRFEQAIRDLAACGNRLFILT
                     GGALHIADTPDAAVCRVALGDEVYGLALRDCVTAACVADGMLVCVKGEAHE"
     gene            complement(6511..7230)
                     /locus_tag="PLZ74_03585"
     CDS             complement(6511..7230)
                     /locus_tag="PLZ74_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97436.1"
                     /translation="MPDGFQRVPRFRARITHRPVLFWWQTCLVLAVFSLLWFELPPEA
                     VFYAPRAFAPLPAPHAAYVSLDPEYATEMFRKTVMGWMLGGVIGKDEGGMEFGLSVLS
                     DALPPPVLLEQGTRYPGVWQPLEVDLPAVQPSVLPAPGGAASCAPRVLAPPPEGVRVV
                     PDAALRAAGWEVAVPDGVLTVNEGHGRFYVETDADGHVAHVLVFPPHTPSTAALERVL
                     ARGRARGAARGIVEVHWRRTP"
     gene            complement(7235..7750)
                     /locus_tag="PLZ74_03590"
     CDS             complement(7235..7750)
                     /locus_tag="PLZ74_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97437.1"
                     /translation="MVSEKRESWSRQVRRHRTVYRFGGAWAQALLSAIPWVTTLLLAL
                     LLMLLHGRLAITPGVAFDLPTAPLREGSRARLTALMLQVTRGDLPSGEETLIFFDDDR
                     YVAQDPEQQLMLAGRLQDRMTLSVQRELVLLADKRVPHGEIMRFVQTARKAGAKRVLV
                     AEKPERGLERE"
     gene            complement(7761..8381)
                     /locus_tag="PLZ74_03595"
     CDS             complement(7761..8381)
                     /locus_tag="PLZ74_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOR97438.1"
                     /translation="MTILMQGGAVFWLIVLLGVAAVIVFFERMLNLWRAQIDYGDFLK
                     GVCNVLDNGNREEAMMICDETPGPVAAVVLTAIRHSQGTPEAIREAVDNTGRAEISRL
                     ERRLASLAVTCQIAPLLGLFAAILGGIRLLLTLTEQAPVIQSLDLSQSLMQALIAAAA
                     GVLVAATCHVMYALLMVRIERIVLDMEASASEIVAYLTHQNRPPVS"
     gene            complement(8378..10840)
                     /locus_tag="PLZ74_03600"
     CDS             complement(8378..10840)
                     /locus_tag="PLZ74_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR97439.1"
                     /translation="MGWLVAVLPLCAVTAAEWETARAALHDKLYPIAQAHAEKALAEG
                     EAPEIALLILAEALAAQDLPQELLRRLDAHPEWIQRAPQPEAFAYWRAWALMRLGQPL
                     EAARIAELAAAEPSSYADALRRLAARARLAAGDQAGALTLFATVDRTSTNRATRVENA
                     LDWALALEQAGRTEAALEVLKAQAEHAESGTAMSEGALLRGRLLFNLGQTSEATRIFN
                     QLAMNERATGSARVQALVEMSVYTLQEGKTNEALAYARSAYDRARQPESRLLAGFRLG
                     DLLSASPDTIDEAEKIIKALVREFPEHPCAMRAHLKLADALLQANRPERAADEYRIFL
                     ETYPPSSLHAEVLQGRGWALMRLGRYAEAGGMFQRASETATNLAEKAECIFKQGDALL
                     ADGRFAEASAAYAKMAGQFPQSPLAGRALYQSAECLERAGRRAEAGQQYRHVAQTFSD
                     REEAPRALLRYAAMQAEDNALEEAVKTYTDVLEAYPQPAVRADALMGRGKALYRTYRF
                     DAAMQDFASVAESTPARRDEARFLLTQCLYGLGRDREAHAAGTAFLLAFPESDFLPDM
                     MLWLGKTTFNSGAYEEASRFFLDYVTRWPAGRWADAALLWAARAAAAKMDFTGAVELV
                     TRLVRAYPQSARLTEALRVQGDALMELARFDEAILLFDQILLKAPAGDEAKQALLRKA
                     DCLFALGAGNSLRYQEALEAYQTLLAENDLAPALTLQLHFKAGRCLEKLKRVDEAIDR
                     YYADVMLRYQDARSRGVWFDATGDSLFVRAAFNVADLYEQKGQPEQAVPVLRRVRDSG
                     MPGSEEAQRRIERLHQTKMGGI"
     gene            complement(11139..11606)
                     /locus_tag="PLZ74_03605"
     CDS             complement(11139..11606)
                     /locus_tag="PLZ74_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97440.1"
                     /translation="MRKRLCALMILMAGGAARGWEEPSVDLFAGWWVSPFRYGAYPYG
                     VFPWRCPDPVVGLGVPLYFRDGRAHDPYFADWGCYPYGWGYGQRVRLRLVEPRYAPAL
                     SDELSLPLPGSAPTELRDPERERAWEREFLRILGDRDEAAWRPDATNGCARSP"
     gene            11773..14187
                     /locus_tag="PLZ74_03610"
     CDS             11773..14187
                     /locus_tag="PLZ74_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97441.1"
                     /translation="MSLTSVLKTARLHARRWTAGWLLCGAATTGLAVTMTVAPNGNDT
                     NPGTAERPLASLTAARDAARQAGAGPHRIVVLPGTYYLEQPLTLDARDNGLTIEAAET
                     NRVTLYGGRRVTGWKREGDTLWVADLPGVKEGTWDFRALVVADRLAERARFPATNTLE
                     NLGTWNLRLLPAVAGHWERKPTKEEATTMPYKPEDLPPTLDIRNAEIRLYHMWSESLV
                     GVVSNDLQRHALILSPAPAWPPGALKRRKYLVFNTREGMTRPGQWYLDRTAGRLVYWP
                     LPGEDMTRLHVVAPTVERVIDLAGTARAPVRNITLRGLSVQATTAPLKLASFGAAAFD
                     GAVHLTRAHECVLEALQIRNVGGVGLKASDLNASRITYCDISDIGACGAVISAKDSLI
                     GHNHIHHLGRYYPAAAATSLRGDRIRIFRNEIHDAPYSGIISGGKENRIEENLIYRVM
                     RELHDGAAIYGNMNACVIRGNVVRDVVEVGKGFGASAYYLDEGARDCIVENNVAIGVP
                     MPTHNHITRNITVRNNVFIAERDMTVSFQRSVGCAFENNTLCFPGKLRLSHPNGIRSW
                     KGNLLFYEGLDQQGNPRRHTICDAMPHFDPPGRKTHAAVAVRVAHPPKLDGVITPDEW
                     PGDLQALNREPSRFTTGGAPTLAKFAYDDTHLYVAANVTMFGPAVVSTNAVWAKDDGV
                     EICLAGQTPDGKPATFVIRGFAGGALLSVTEAGAPAEAAARVGRESRFVVQSAPGPGN
                     RLFGRGWRGEWAIPLAALGIRPTPGLKVPFNMAAYYSEFDEWRCWEGTQAENWRLDQA
                     GMLEFK"
     gene            complement(14665..16647)
                     /locus_tag="PLZ74_03615"
     CDS             complement(14665..16647)
                     /locus_tag="PLZ74_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOR97442.1"
                     /translation="MAVLLPVERKTMKARVFLALIYAVLSLGGITMVWPFLVMLASSL
                     TGPYDYYRHAPVVRAMWDRNDRFMRYLATCFPRFPSTVYPDAPTHWGSWTTVAREGVA
                     CRAWAAEQLKGLADPVTAERWRRMAEDYAAFNRTYEIANTVCHFDPRDVAGFVRRHYE
                     ERVRDEDPQAYNRMSPKARRQAALARLNAEWPVRYTSFFGIRMMAQQRAPLHHAGWDE
                     PIGDAKMAVYQTFKAMYREQAAARGVPMSRTRPFESLPLWSAYLRRALPEAADKWDGF
                     FPLPADAPDTVRRVWERFIREAYPRRLLRVRVTPERERAYRAYVRSVCRTPEAYAQLT
                     GRSLPPPDPSDTSEAAAFSGLPLPAYENSTLWRNFIPSVPLAELSVRSAEQAWQAFLL
                     ERYGSTEGVREAYGLPHARIEAARLPLREALAVTFARRGGRDFLDSMLANYRLVGEYL
                     FLRGQAFGNTLLLVVLSILATLTVNPLAAYALSRFGLRRAENILLFLLATMAFPAAVT
                     AIPGFLLIRDLGLLNTFAALVLPTLASGMSIFILKGFFDGLPRELYEAAAIDGAKEWQ
                     ILLRITLPMTTPILAVNALTAFVHAYNSWEWALLVCQKPSRWTLAVWMYQMSQQLSDQ
                     PWAVMAGFVLVSLPTALVFFACQKVILRGIVLPSMK"
     gene            complement(16682..16837)
                     /locus_tag="PLZ74_03620"
     CDS             complement(16682..16837)
                     /locus_tag="PLZ74_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97443.1"
                     /translation="MPFCGDYTEAAVWSGAQWTKHKRDQAKRDRADAVDRATLEALAR
                     ETRRTGL"
     gene            complement(16874..16996)
                     /locus_tag="PLZ74_03625"
     CDS             complement(16874..16996)
                     /locus_tag="PLZ74_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97444.1"
                     /translation="MLNWISAGSTVKALPLSVRGSRVSKLFTMLDQGHAKGITP"
BASE COUNT         3448 a         5905 c         5502 g         3039 t
ORIGIN      
        1 cagcacggct gacacgagcc tgagcagcga ggcctcgttg gggaagagcc ccgcgacccc
       61 gtccccccgg acaggaccga gtcccgcttt gacagctaca aaccgtccag ttttgttttg
      121 acctcgacaa gacgcttttt gtcctgtctg cgtccccggc gttttgttat agttcggcat
      181 tctgttgtat taacattcgt taaaggggga gcggatatgc gtgagccggg aagattcgga
      241 tggatggtcg gactgatggc ggcaatggcg gttgcggggg caagcgcgca aacggttttc
      301 catgtggacg cgcagcgcgg gcaggacacg caggatggta gcgccgcccg tccgtttgcg
      361 agcctgaaac gggcccagac ggcgatccgc gagctcaaaa cgaatcagac gtttccggag
      421 aagggcgtga cggtcgaggt cagcggcacg ttcgtcatgc ccgaggagac ggtgtctttc
      481 gaagaatcgg acagcgggat cgggccgaac gcgcccgtaa tcgtgcgggc cgcgaaagcg
      541 ggcgcacttt tcctgggcgg acggcgattg ccggccgaag gcttcaagcc ggtttccgat
      601 gcggccacgc tggcgcgcct gccggaggcg gtgcgcggca aggtggtggc gttcgacctc
      661 aagaccatcg gcgtcacccg gctcgcgccc ttgccggaca agttccacgg ctggcgcgaa
      721 atggagcttt tcagcaatgg caaggcgatg cggctcgcgc gctggcccaa cacgggctgg
      781 gcacaaatcg ccaaggtgat cgaccgggga gtgaagccaa tagaccgggc caccggtgaa
      841 tgggaattcg gtgtgcgcgg cggcatattc gagtacgccg aggacgcgcc cgcacgctgg
      901 gatgtctcca aaggaatctg gatgaacggg ttctggtgcc acgactgggc caacgagacc
      961 ctccgcatcg gcgcgatcga caaagagaaa aaacagatca cctcggccgc gatccatact
     1021 tacggcatcg gcaactcctc caagtggcat acggccaaac ggcgttacta tgttttcaac
     1081 ctgctggaag aactcgatat gcccggcgag tggtatgtgg accgtgaggc gtgcctcctc
     1141 tacctctatc caaccgacag cggccttggc gatgtcgtgc taaccgatca aaagaagccc
     1201 ctgttccgtc tgaacaaagt gcggtatgtt cgactggacg gactgacctt ccgttacaac
     1261 accggtctgg ctgtttccgt gaacggatgc gaagaggtgc ggcttgaaaa tctgcgcgtc
     1321 tcggacatca ccctggacgg gatccacatc acgggcggac gggcctgcgg cctgaaccgg
     1381 tgcgaggtgt acggcgtggg aggcacctgc gtttcggttt cgggtggcga ccgcaaatcg
     1441 ctgaccgcct gtgaccattt cgtcaccaac tgccgcctgc atcacagcgg gcggctacag
     1501 cgtacgcagg gcaagtgcct caacttcaac ggcgtgggga tccgtatcgc gcaaaacctg
     1561 atctatgacg cgccgtacat cgcggtgacc tatggcggca acgagaatct cttcgaatac
     1621 aacgaggtgc attcggccat gatggagagc ggagacggcg gcgggctcta taccgggcgc
     1681 aactggtgct cgcaggggaa tgtgatccgc cacaactatc tgcatcactt cggccagcct
     1741 ggcgtggatt ggcagaaggc gcgggggctg aatcccgatt acgagccgct caaggagtcc
     1801 gtgatggtca tgggcgtcta tttggacgac tgcgacagcg gcgatacgat ctgcgacaac
     1861 atcttctacc gcgcgggctg ggccgccttc gtgggcggcg gacggtataa cacggtccgg
     1921 aacaatctgt ttatcgagtg cacctccgcg ttgcacctgg ataatcgcgg cctcaagcgc
     1981 gcgcgtccgg gcgagggcac gaaagacggg tgggacctgc tcgccaaact tcaggcggtc
     2041 aagtggcagg agtccccttg gaaggataaa tatccgcatc tggtgaacat catggaggat
     2101 gagcccaagc tgccgctgca caacgtcttc cgcgagaacg tggcggtcaa ctgcaaactc
     2161 ttcctgaacg cgaacaacgt ggtgcgcacc ggcgtcgtgc accgcctgac gttcgagaac
     2221 aacctctcgt tcgggccgat cgacaagcgc gatgtgatgg ctttccccca gaccgatgag
     2281 ggcaagcgcc gggtgacgtt cctggagacc ccgctgccgg acagtgtcga cccggatacg
     2341 aacggattcc ggattcagga cagcggggag ttcaagcgtc tggcaccctg gttcaagcgc
     2401 atctcgctgg agcggatcgg accggaaaag aaaaattaag gctgttcgcc aaacatgccg
     2461 aagggatttc atgggacgcg ggggtgtccc gcaaacaaca cgcgaaaaaa aggagacgga
     2521 atgaagaaaa tgcggaagtt ggggtgggta ggcgcgatac tgcttgccgg ttcggttcag
     2581 gctgcggttc ggatgccgaa aattttcggg gacggcatgg tcttgcagca acaggttgcg
     2641 gtgccggttt ggggatgggc cgcgccgggt gagcgggtaa ccgtcacctt tgcgggacag
     2701 agcaaggaga ccgcggccaa ggcagacggc acatggtcgc tgaaacttga tgcgctccaa
     2761 gcctcgtccg agccggcccg gctggaggtg cggggcgcga acgcgctgac cttttcggat
     2821 gtggtggtgg gcgaggtgtg gttctgttcc ggccagtcaa acatggagtg gaccatgcgc
     2881 tccgtagacc atgcggagaa ggagatcgcg gcggccaacc tgccgctgat ccgccatttc
     2941 aaagcgccca aacgggtctc gggactgccg ctggatgacg tgaacgccgt ctgggaaaag
     3001 acctcccccg ccgggatcgg cgacgagtcc gcggtagcct ttctgttcgg acgccgcctg
     3061 catcaggtgc tcaacgtgcc catcgggctg atcaactgtt cttggggcgg cacgcggatt
     3121 gagccctgga ccccgctctg tggattcgag gggatcccgg cgctggaggg gattctccgc
     3181 aatgcgcaga aacgggtcga gcaagtcagt cccggctcga cgtcgtacaa agccacgcac
     3241 cagacctatt tcacccggct gcaggagtgg atcgcggaga gcgaaaagcg gctcgccgcg
     3301 aatcaggcgg tgaccgcgat gccggaatac ccctcggtgt tctccttccg gaatcaggat
     3361 cccacagtcc tgtttaacgg tatggtcgcc ggactggtgc cctatgcgat ccgcggggca
     3421 atctggtatc aaggctgttc gaacaacggt gaaggcatgc tttatctgga gaaaaccaaa
     3481 gcgctcgtca agggctggcg ccaagtgtgg ggacaaggcg atttccccta ttacctcgtg
     3541 cagctcgcgc catacacgta tggaggcaac cctgagaatc tgcccggcat ctgggaggcc
     3601 caggcggccg tgcccgccgc gatccccaac accggctaca ccgtgatcaa cgatatcggg
     3661 aacatccgcg atatccatcc gcgcaacaag caggatgtcg gcctgcgtct cgccaatcag
     3721 gcgctcaacc gcacttacgg gcgcagcgaa atcgcctggg ccggtccggt ttaccgctct
     3781 ttcgccatcg agggccagac ggtgcgcgtc accttcgatc acgccgaggg gctgaaaacc
     3841 cgcgacggcc agccgccgac ctggtttgag atatgcgggg aggacggccg gttcgtcaag
     3901 gctgacgcca agatcgacgg aacgagcgtg atcctttccg cgtcgtccgt cagcgctccg
     3961 aaagccatgc gcttcgcgtg gaaccagatc gccgagccga atctggtgaa cggtatcgga
     4021 ctgcccgctg gcgcgttccg ctgcggcaaa tgatcgacat tccggcaccc gggccgcggc
     4081 gtaccgcggc ccgggtccgc catcacgaac ccggatgcac gaacggttcg ttccgcgcgc
     4141 agagcggaca ggccgcgggc tcccaaacct caggcgccat gtgcaacagc gagaaggtcg
     4201 gatacggaaa cacggcctgg cccgcgctgc ggtccaccat caccagcacg gccaccactt
     4261 ccgcgtccgc cgccttcacc agatcgatgg tttcctgcac gcgcccgccg cgggtgatca
     4321 cgtcttccgc aaccacataa cgctctcccg gtttgagcgt aaaacgccgc atcaccagcg
     4381 cgccgtcctg tttctccgcg aacacgcact tcacatccag cgcgcgcgcc acctcatggc
     4441 ccaccagaat gccgcccagc gcgggtgaga tcacgccgtc cacccccccg tccagggctt
     4501 tcgcggcgcg ggcgcgcgcc gtcagctctt cgcaaagccg accggccacc ctcggataac
     4561 gcagcacatt ggcgcattgg aaaaagcggt tgctgtgcaa ccccgaccgc aattcaaagt
     4621 gcccgctcag cagcgcgccc gtctcttcca acgttttcag aatctccttg tccgtcatgg
     4681 tccggtttcc tctctcacgg ttccgttttc aagtttcacg cgccgtgcca tacgccgtgc
     4741 gcacaactcc gagtgcgtga ccacaatcag cgtggcacgc cgttcggcat tcagttccag
     4801 cagcaggtcg atgagcgcgg cggcgttttt ctgatccagc gcaccggtcg gttcgtccgc
     4861 caataccagt cccgggtcca tgaccagcgc ccgcgccacc gccacccgct ggcgttcgcc
     4921 gcccgagaga tgccctggaa aatggcgcat ccggtcgccc aggccgaccc gttccagcag
     4981 atgttccgcg cgctcccgcg cagtcgcatc cggtccggac catgcgggga cgagcacgtt
     5041 ctccagcgcg gtcagttgcg gtagcagatg gtgattttgg aacacaaacc ccacctcccg
     5101 gttacggtat cgcaaccgcg ccgcttcctc aagcgcgtcg agccgctgac cgttcacccg
     5161 gatttcaccc gcatccggcg tgtccagcgc accgatcaga tggagcagcg tggtcttgcc
     5221 ggaaccggaa gggccggtca cggccacgct tccgcccgcc gtaaccgtca gatccgcacc
     5281 gcgcaacact tcgaccggca ccccccccgg cggacggaag gtccgcacca cacccgtcac
     5341 cagcacatcc gcgccgttac tcatgagcct cccctttcac gcagacgagc atgccgtccg
     5401 ccacacatgc cgccgtcacg caatcgcgca gggccaaacc gtacacctcg tcgccgagcg
     5461 ccacacggca aaccgcagca tccggcgtat ccgcgatgtg cagcgcaccg ccggtcagga
     5521 tgaagagccg gttaccgcaa gcggccagat cacggatcgc ctgttcaaag cggtattccc
     5581 cggcctgcga aagcgttgta tcccgcagga accacaagga aaccaagccc tcttcgctac
     5641 ccgtcgcgat cagcccgttt tgggtccgga ccggtttgag aaacgcctca ttctccgaaa
     5701 cccgcgtccg cgcctcgcac acgaaagtcc gcgcgttcgc caccaccaga tttccctgcc
     5761 gcgtacccgc caccagtcga ctatccggca acgagagcag accgccggcc atctgatcct
     5821 ccgtgcccac cgggatcgat cctttacgcc gaccgtccgc ggcatcgaac acatggatcg
     5881 cgccatcgca attcccgtaa gcgatcccgt acggtccggc ggctggctcg ccgtcgcacc
     5941 ggttggtcgg ttcactctgc cagagggtca ggccgtcacg aacgcgcaga cagaagagcc
     6001 gcccgtccga ctgcgacacc agacgcatca ccgccgcatc cccgccgccc tgcgcgtcga
     6061 gcaacggcgg atgctggaaa cgcgcgtcga gcgtcacaga ccagagcgca cggccggttt
     6121 cggcatccag cgccagaacc ccgccactgg tggaggcagc gaacacccgc cgatcgtgta
     6181 tcgcggccga ggcttcgaac ggctggttgg aaaaagagac acgccacaat ggctttcccg
     6241 tatcggagag gcgggtcaca ccgcccgcat cgtccgtcac gatccagcct tccgcgtcca
     6301 ccggaaccgg cgcgttggcg tgcgccgtga gtgccgcgcg ccaaaccaca cgccatgcgt
     6361 tccgaaccgc gggttgcgcc gcagaaggca cgggctgcgg cgcgcacatc gaacgcgcga
     6421 gccagccgat gacggcgacc gccgctatga ccgcagcgac ggcaacgtac gccgtggccg
     6481 ggcgccgact cgggataggg cggcgtgaca tcatggcgtt cgtctccaat gaacctccac
     6541 gatcccgcgt gcggcaccgc gggcacggcc acgcgcgagc acccgctcca gcgcggcggt
     6601 ggagggtgtg tgcggcggaa aaaccagcac atgcgcgaca tgcccgtccg catcggtttc
     6661 cacatagaag cggccgtggc cctcgttcac ggtcagaacc ccgtctggca cggccacctc
     6721 ccatcccgcc gcgcgcaagg ctgcatccgg cacaacccgc acgccctccg gcggcggcgc
     6781 gagcacgcgg ggcgcgcagg atgcggctcc ccccggcgca ggcagcaccg acggctggac
     6841 tgcgggcagg tcgacctcga ggggttgcca cacgcccgga taccgtgtgc cctgttccaa
     6901 caggacgggc ggcggcagcg cgtcgctcag caccgagagc ccgaactcca tgccgccctc
     6961 atctttgccg atcacaccgc caagcatcca ccccatgacg gttttgcgga acatctcagt
     7021 ggcatactcg ggatccagag agacatacgc ggcgtgcggg gcagggagag gcgcaaacgc
     7081 gcgcggcgca tagaaaaccg cttccggggg aagttcgaac cagagcaggg aaaagaccgc
     7141 cagcaccaga cacgtctgcc accagaacag gaccggccgg tgcgtgatgc gggcccggaa
     7201 acggggaacc ctctggaaac catccggcat gccgttactc ccgctccaag ccgcgttccg
     7261 gcttctccgc caccagcacg cgcttcgcgc cggccttgcg ggccgtctgg acgaaacgca
     7321 tgatttcgcc gtgcggcacc cgtttatccg ccagcagcac cagttctcgt tgcacgctca
     7381 gggtcatgcg gtcctgcaag cgtccggcca gcataagttg ttgttccgga tcctgcgcca
     7441 cgtagcggtc atcgtcgaag aaaatgaggg tttcctcgcc cgagggcaga tccccgcgcg
     7501 tcacctgcaa catcagggcg gtcaaccggg cacgcgatcc ttcgcgcaac ggcgcggtcg
     7561 gcagatcaaa ggcgacaccc ggcgtgatcg ccaaacgtcc gtgcagaagc atcagcaaaa
     7621 gcgccaacag aagcgtcgtc acccacggta tggctgacag cagcgcctgc gcccaagcgc
     7681 cgccgaaacg gtagaccgtc cggtggcgtc ggacttgccg gctccaactc tctcgtttct
     7741 cgctaaccat acacagcctt ttatgatacc ggagggcggt tctgatgagt gaggtacgcc
     7801 acgatctccg aggccgaagc ctccatatcc agcacgatgc gttcgatacg caccatcagc
     7861 aaagcgtaca tcacgtgaca ggtcgcggcc acaagcacgc ccgcggcggc ggcgatcagc
     7921 gcctgcatca agctctgcga aagatccaga ctctggatca ccggggcctg ctccgtcagc
     7981 gtcagcaaca gccggatccc gcccagaatc gcggcgaaca atcctagcag cggggcgatt
     8041 tggcaggtga ccgccagcga agccagccgc cgctccaaac gcgagatctc ggcacgcccc
     8101 gtgttgtcca ccgcctcgcg gatggcctcg ggtgtgccct gactgtgacg gatcgcggtc
     8161 aagaccaccg ccgcgaccgg gccgggcgtc tcgtcacaga tcatcatcgc ttcttcgcgg
     8221 ttgccgttat caagcacatt acagacccct ttcaggaagt cgccgtaatc gatctgcgca
     8281 cgccacaggt tcaacatccg ttcaaaaaac acgatcacgg cagccacgcc caagaggacg
     8341 atcagccaaa acaccgctcc gccttgcatc aggatggtca tatgcccccc atcttggttt
     8401 gatgcagccg ttcgatccgc cgctgcgcct cctcgcttcc gggcatgccc gaatcgcgca
     8461 cccgccgcag caccggaacc gcctgttccg gctgcccctt ctgctcataa agatccgcca
     8521 cgttaaacgc cgcgcgaacg aacaggctgt cgccggtcgc gtcgaaccaa accccgcggg
     8581 agcgcgcgtc ctgatagcgc agcatgacat cggcgtaata acggtcgatc gcctcatcga
     8641 cgcgtttcag tttctccaga caacgcccgg ccttgaaatg gagttgcaac gtcagcgcag
     8701 gggcgaggtc gttctccgca agcagggtct gatacgcctc cagcgcctcc tgatacctca
     8761 ggctgttgcc cgcaccgagc gcgaacaggc aatccgcctt gcgcagcagc gcctgtttgg
     8821 cctcatcccc cgcaggcgcc ttcagcagga tctggtcgaa gagcaggatc gcttcgtcga
     8881 accgggccag ttccataagg gcgtcgccct gcaccctcag tgcttcggtc aggcgcgcgc
     8941 tttgggggta ggcgcgcacc aaacgcgtga ccagttcgac cgcccccgtg aaatccattt
     9001 tagcggctgc ggcgcgcgcg gcccaaagca gcgcggcatc cgcccaccgt ccggcgggcc
     9061 agcgggtgac gtaatcaagg aagaagcgcg acgcctcttc gtatgcgcca ctgttgaagg
     9121 tggtcttgcc cagccacaac atcatgtcgg gcaggaaatc ggattcggga aaggccagca
     9181 ggaaggcggt gcccgcggca tgggcctcgc gatcgcggcc gagcccgtag aggcattgcg
     9241 tcaacaggaa gcgggcctca tcgcgccgcg cgggcgtgct ttccgccaca ctggcgaaat
     9301 cctgcatcgc cgcgtcgaac cggtaggtgc ggtacagggc tttgccgcga cccatgagtg
     9361 cgtcggcgcg taccgccggt tgcggatacg cctccaacac gtcggtgtag gttttcacgg
     9421 cctcctccag cgcattgtct tcggcctgca tcgcggcgta acgcaacagg gcgcggggcg
     9481 cttcttcgcg atcagagaag gtctgcgcca catgacggta ctgctgaccg gcctcggcgc
     9541 gccgtccagc gcgttcgaga cactcggcgc tctggtagag cgcgcgcccg gccaaggggc
     9601 tctggggaaa ctgaccggcc atcttggcgt aggcggcgga agcctcagcg aaccggccgt
     9661 ccgcgaggag cgcgtcaccc tgcttgaaga tgcactcggc cttttccgcg aggttcgtgg
     9721 ccgtctcgga agcgcgctgg aacatgccgc ccgcctccgc atagcgaccc agccgcatca
     9781 gggcccagcc gcgcccttgc agcacttcgg catgcagcga agagggcgga tacgtttcga
     9841 ggaagatgcg gtattcgtcc gccgcccgtt ccgggcgatt ggcctgcaac aacgcgtcag
     9901 ccaacttgag gtgggcgcgc atggcgcacg gatgttcggg aaattcgcgc accagggcct
     9961 tgatgatctt ttcggcctca tcgatggtat ccggtgaagc ggacaacaga tcacccaacc
    10021 ggaacccggc cagcagacgg gactcgggct gccgcgcccg gtcgtaagcg ctgcgcgcgt
    10081 aggccagcgc ctcgttggtt ttgccctctt gcagcgtgta cacgctcatc tccaccagcg
    10141 cctggacccg cgccgaaccg gtggcacgct cattcatggc gagttggttg aaaatccggg
    10201 tcgcctccga agtctggccg agattgaaca gcaaccgacc gcgcagcaac gcgccttcgc
    10261 tcatggccgt acccgattcg gcatgctccg cctgcgcttt gagcacctcc agagcggcct
    10321 ccgtgcgacc ggcctgttcg agcgcgagag cccagtccag cgcattctcc acacgggtgg
    10381 cgcggttggt cgaagtgcgg tctacggtgg cgaagagcgt cagcgcacca gcctggtccc
    10441 cggccgcaag gcgcgcgcgc gcggcgagac gccgcaacgc atcggcatag gacgacggtt
    10501 cggcggcggc aagctccgcg atgcgggccg cttccagcgg ctggcctagg cgcatcaggg
    10561 cccaggcgcg ccagtaggcg aaggcctccg gctgcggagc gcgttgaatc cattccggat
    10621 gcgcatcgag ccgccgcagc agttcctgcg gcagatcctg ggccgcgagg gcttcggcca
    10681 gaatcagcag cgcgatttcc ggcgcctccc cttcggctag ggccttttca gcatgcgcct
    10741 gagcgatcgg gtacagcttg tcatgcagcg cggcacgggc cgtttcccat tcggccgcag
    10801 tcacggcaca caacggcaac accgccacaa gccaacccat cactctgttc catccgctca
    10861 agggcagaca cctctaaaga aagctttccg ttttccggag gaaatccgca cggcggaagc
    10921 cgcccggcat ccgtccggcg ggcttttccc cacacaccat agtttgccat gtttggtttt
    10981 cgcggtcgat acgaaagaca ccgcaccgcg ctctttggtt tcaggttgcg ggttccgccc
    11041 gcgttcatgc cttcgtgtgg gagtcgggaa cgcgggcgtc cccacccaca acggcagatc
    11101 cgtcgccctg ccaactttcc gtctctccac ggcagggatc acgggctccg ggcgcaaccg
    11161 ttggtggcat ccggacgcca cgccgcctcg tcccggtcac cgagaatgcg tagaaactcc
    11221 cgttcccacg cgcgttcgcg ttccggatcg cggagttccg tgggcgcgga accgggcaac
    11281 ggtagcgaca actcatcgga taacgcgggc gcgtaacggg gctcgaccag ccgcaaccgg
    11341 acacgctgcc cgtaccccca tccgtacgga taacagcccc aatccgcgaa gtacgggtcg
    11401 tgggcccgtc catcccgaaa atacagaggc actcccaacc cgacaacggg atcaggacac
    11461 ctccagggaa acaccccgta cggatacgcg ccgtaccgga acggcgacac ccaccagcct
    11521 gcaaagagat cgacggacgg ctcttcccaa ccccgcgcgg cccctcccgc catcagaatc
    11581 atcaatgcgc acagcctttt tctcatggac acagtataca cgacctcccg gccacaaaaa
    11641 agggtgtttg cgcattgact agccgagtga accgcaacat aatggttgca ttcgggggaa
    11701 agggcgagag cctgtccggt cgaggccggc gcgcccctcc tccgtcatta acccgttctg
    11761 gaggaaacga tcatgagtct gacatccgtt ctcaagaccg cccggctgca cgcgcggagg
    11821 tggaccgcag gctggctcct gtgcggtgcg gcaaccaccg gattggccgt tacgatgacc
    11881 gtggccccga acgggaacga caccaacccc ggcacagcag agcggccgct ggcctcgctt
    11941 accgccgcac gggacgcggc ccgccaagcc ggcgctggtc cccaccggat cgtggtgttg
    12001 cccggcacgt actatctcga acagccgttg accttggacg cgcgcgacaa cggactcacg
    12061 atcgaggcgg cggagaccaa ccgcgtgacg ttgtacggtg gacgccgggt aaccggctgg
    12121 aagcgggagg gcgacacgct ctgggttgcc gatctgccgg gggtgaaaga gggcacatgg
    12181 gatttccgcg cgctggtcgt ggcagaccgc ctggctgaac gcgcgcgctt tcccgccacg
    12241 aacaccctgg agaacctcgg cacctggaat cttcgcctgc tgcccgccgt ggccggccac
    12301 tgggaacgta aacccacgaa ggaagaggcg acgaccatgc cttacaagcc cgaggacctg
    12361 ccgcccacgc tcgacatccg caatgcggaa atccgcctct accacatgtg gtccgaatcg
    12421 ctggtcgggg tggtcagcaa tgatctgcag cgccatgcgc tgatcctttc gcctgcgccc
    12481 gcttggccgc cgggtgcgct caaacgcaga aagtatctgg ttttcaacac ccgcgaaggg
    12541 atgacccgtc cggggcaatg gtatctcgac cgcactgccg gacggctcgt ctactggccg
    12601 ctgcccggcg aggacatgac ccgcctccat gtggtggcgc cgaccgtcga acgggtgatc
    12661 gacctcgctg gcacggcccg ggccccggtc cggaacatca cgttgcgcgg actctccgtg
    12721 caggccacca ccgccccgct taaactcgcc agtttcggcg cggcggcgtt cgacggcgcc
    12781 gtgcatctca cccgcgccca cgagtgcgtg ttggaagcgc tgcaaatccg caacgtcggg
    12841 ggcgtcggcc tcaaggcttc cgatctgaac gccagccgca tcacctactg cgacatctcg
    12901 gatatcggtg cttgcggcgc ggtcatcagc gccaaggact ccctcatcgg acacaaccac
    12961 atccaccatc tcgggcgtta ctatcccgct gcggcggcca cctcgttacg cggtgaccgg
    13021 atccgtattt tccgtaacga aatccacgac gccccctact ccgggatcat cagcggcggt
    13081 aaggaaaacc ggatcgagga gaatctgatt taccgggtga tgcgcgaact gcatgacggt
    13141 gccgccattt acggcaacat gaacgcatgc gtcatccgcg gtaacgtggt acgcgatgtg
    13201 gtggaggtcg gcaagggatt cggcgcctcg gcctactatc tggacgaggg cgcgcgcgac
    13261 tgcatcgtgg aaaacaatgt cgccatcggt gtcccgatgc cgacccacaa ccacatcacc
    13321 cgcaacatca cggtgcgcaa caatgtcttt atcgcggaac gggacatgac cgtctcgttc
    13381 cagcgttcgg tggggtgcgc ctttgagaac aacaccctct gtttcccggg aaagctccgt
    13441 ctatcgcatc cgaacggcat ccgaagctgg aaaggcaacc tgctctttta tgagggcttg
    13501 gatcagcagg gtaacccccg gcgccacacg atctgcgacg cgatgccgca tttcgacccg
    13561 ccgggccgca aaactcatgc cgcggtcgcc gtgcgcgtcg cgcatccgcc gaagctggac
    13621 ggggtgatca cgccggatga atggcccggg gacttgcagg ccctgaaccg cgagccgtca
    13681 cggttcacca ccggcggagc gcccacgctc gcaaagttcg cctatgacga cacccacctc
    13741 tacgtggccg cgaacgtgac catgttcggc ccggcggtcg tgagcacaaa tgcggtctgg
    13801 gccaaggatg acggtgtgga gatctgcctt gcgggacaga cccctgacgg aaagcccgcc
    13861 actttcgtga ttcgcggatt cgcgggaggc gcgctgttga gcgtcacgga agcgggcgcg
    13921 cctgccgaag ctgcggcgcg ggtaggacgc gagtcgcgtt tcgtggtgca gtccgcgccg
    13981 ggaccgggca accggctctt cggacgcggc tggcgcggcg aatgggcgat acccctcgcc
    14041 gcgctcggca tccggcccac acccggcctg aaggtgccct tcaacatggc cgcgtattat
    14101 agcgagttcg atgaatggcg ctgctgggag ggtacgcagg ccgagaactg gcgtctcgat
    14161 caggcaggga tgctggagtt caaataaccc tgtcgggatc ggattgtttc ccccgtccgt
    14221 gttccgtgtc ccagtggacg tcgacctgcg gcccgcgaca ttcgacccgc ggcgtttttt
    14281 gatgaggcgg cggtttcggt gaaaccgcct tgtcagcagg cgggggacgc ctgtttcgtc
    14341 ccgatccttt tcggcaaaaa agaacggcgg ataaaagttt tttactttta tccgccgcca
    14401 gggaggacat atataattga gatgcgccct acagggggga acctgtattc gcgatcgcgg
    14461 gggaagcgtc gcgctaggcc gcatcgttca tcaacctggg ggaggttgtc tgaacggggt
    14521 gtatagtata tgacgcaccc caaagtgtca aggggggggc ggtgtttttt tcggaccccc
    14581 gcgatccccg cttcgggaca ggtcgaaacg cttctcgcgc cggtcgcgcc acggatttta
    14641 cggctgcata ctcccgaccg catttcactt catgctcggc agcacaattc cgcgcaggat
    14701 caccttctgg caggcgaaga acaccaacgc cgtagggaga gagaccagaa cgaaccctgc
    14761 catcacggcc cagggctggt cgctgagttg ctggctcatc tggtacatcc acaccgccag
    14821 ggtccaacgg ctcggtttct gacagaccag taacgcccac tcccaactgt tgtaggcatg
    14881 aacaaacgct gtcagcgcgt taaccgccag gatcggtgtg gtcatcggca gggtgatgcg
    14941 taaaaggatc tgccactctt tggcaccgtc aatggctgcg gcctcataca gttcgcgcgg
    15001 caggccgtca aaaaaacctt tcaggatgaa gatcgacatg ccgctggcca gagtgggcag
    15061 caccagcgcc gcaaaggtgt tgagcaggcc cagatcgcgg atcagcagaa agccggggat
    15121 ggccgtgact gcggcgggga aggccatggt ggccagcagg aagagcagga tgttctcggc
    15181 ccggcgcaga ccgaaacgcg agagcgcgta cgccgccaac ggattcaccg tcagcgtggc
    15241 gagtatggaa agcacgacca gcagcagcgt gttgccgaag gcctgcccgc gcaaaaaaag
    15301 atactccccc accagacggt aattggccag catgctgtcc agaaaatcac gcccgccgcg
    15361 gcgcgcgaag gtgacggcca gcgcctcgcg caacggcaac cgcgccgcct cgatccgcgc
    15421 gtgggggaga ccgtaggcct cccggacgcc ttccgttgaa ccgtaccgct ccagcagaaa
    15481 cgcctgccac gcctgttccg cactccgcac actcagctcc gcgagcggca ccgaggggat
    15541 gaaattacgc cacagcgtac tgttctcata agcaggcagc ggcaggcccg agaacgccgc
    15601 agcctccgaa gtgtcggacg gatcgggcgg cggcagcgac cggcccgtca gttgcgcgta
    15661 agcttcgggc gtgcggcaca cggagcgtac gtaagcccgg taggcgcgtt cgcgttccgg
    15721 ggtcacccgc acgcgcaaca gtcgtcgcgg gtaggcctcc cggatgaacc gctcccacac
    15781 ccggcgcacc gtgtccggcg cgtcggcagg cagggggaaa aaaccgtccc acttatccgc
    15841 agcctccggc agggcgcgcc gcaggtaggc ggaccacagc ggcagcgact caaagggccg
    15901 tgtccggctc atgggcaccc cgcgcgctgc ggcctgttcg cggtacatcg ccttgaatgt
    15961 ctggtaaacg gccattttgg catccccgat cggctcgtcc cagcccgcat ggtgcagggg
    16021 ggcgcgctgc tgcgccatca tgcggatgcc gaagaaagag gtgtagcgca ccggccactc
    16081 ggcattcagc cgcgcgagcg cggcctgccg ccgggccttg ggcgacatcc ggttgtaggc
    16141 ttggggatct tcgtcgcgca cccgctcctc gtaatgacgc cgcacaaacc cggccacatc
    16201 gcgcggatcg aagtggcaca cggtgttggc gatttcatag gtacggttga aggcggcgta
    16261 atcctccgcc atgcgccgcc accgttcggc cgtaaccgga tccgccagtc ccttgagctg
    16321 ttccgcagcc caggcgcggc acgccacgcc ttcgcgcgcc accgtggtcc aacttcccca
    16381 atgcgtcggg gcgtcgggat aaaccgtgga ggggaaacgc gggaaacaag tggcgagata
    16441 acgcatgaag cggtcattgc ggtcccacat cgcgcgtact acaggggcat ggcggtaata
    16501 gtcgtacggg ccggtaaggg aagaggccaa cataaccagg aaaggccaga ccatggtgat
    16561 tccgccgagc gacagcacgg cgtagatcag ggccagaaag acccgcgcct tcatcgtttt
    16621 ccgttctacg ggcaacagaa ccgccatgcg attccccctc ttttctgagc cgatccgccg
    16681 cttacagtcc cgtgcggcgc gtctctctgg ccagcgcttc caacgtggcg cgatccacgg
    16741 catcagcccg gtcgcgctta gcttgatccc gtttgtgctt cgtccactgc gccccactcc
    16801 aaaccgcagc ctcggtgtag tcaccgcaaa agggcacacc gagatcggtc cagagcgcaa
    16861 ggagcgcctt ctcctaaggc gtgatcccct tggcgtggcc ctgatccagc attgtgaaga
    16921 gcttgctgac gcgcgatccc cggaccgata ggggcaacgc cttaacggtc gatccggccg
    16981 agatccagtt cagcaccgcg tgatccgcat ctcccctgta tgctattcac tataacaaac
    17041 accgtccgtc ttggcgaagc cgaaaaaccc gtaattccaa cacgggtgag gtctggcgaa
    17101 gccgaatagg ttatgggtgt tcggcacgtc attgttgtcg aaaacaggtt cccccttttt
    17161 ttccaatcag gcaagggatt tctggcagtc cagacgttct ctctccgtct ttgcgctgac
    17221 ctcagacctc gcccctcctc tgcccacata tcatgagaag tggacagccc gtcgcatgct
    17281 ccaacgagat ctaaaaaacc atgaaaccca taaacttcat gaaatcaagg acgggcagaa
    17341 aggcgagagc gcggacgtcc tcgcccgcaa cccggtttcc ggttttatcg ctatcggaag
    17401 cgcttccccg taatcatccc cgttcgcgtt ttcgtgaacg tcgccctacg acccgcgaca
    17461 gaccacatgc gacaggaacg cctccaatcc gactgatccg ctgttgcgga cgggggacgc
    17521 ccacgttccc gactcccgct agtcgctatg aatccggcca caaaccgcat tgccctttga
    17581 acaccaagat gcccctgctt ttcatgaagg ttccgtttta gggtttcagg gtttcaaggt
    17641 ttcatctaaa tcgggatcgg ggtcgggatc gctatcggga tcgggatcga ctgtttcacc
    17701 acgggacact ttcttgggtt tcagggtttt agggtttcaa ggttttaggg tttcaaggtt
    17761 tcagggtttc aaggtttcag ggttcatcca aatcggtatc ggggtcggga tcgctatcgg
    17821 gatcgattat ttaaccacaa actcacggga tggcactgct cgggttttta tccacagatt
    17881 tacacagatt acgc
//
LOCUS       DAOSRB010000039        17773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3308_length_17773_cov_6.489784, whole genome shotgun sequence.
ACCESSION   DAOSRB010000039 DAOSRB010000000
VERSION     DAOSRB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17773
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3308_length_17773_cov_6.489784"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLZ74_03630"
     CDS             complement(<1..361)
                     /locus_tag="PLZ74_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97445.1"
                     /translation="MKMRLREWMFGLVLSMFVLTLHATTTNIYVWCGSAGDGDWFNAA
                     NWSLTVDGTPIDPPEVPGSRYLTDGTANVGVYIVAASNVTGTINYTPPPGAFLTNTFL
                     SIEKSASDLTPLTFNVNG"
     gene            complement(413..2083)
                     /locus_tag="PLZ74_03635"
     CDS             complement(413..2083)
                     /locus_tag="PLZ74_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97446.1"
                     /translation="MKPVVVMTGVAVGWIVFSAAAETNYWIATSGTGNWNEPANWSLD
                     RVPGAEDTVIITNAATTGNGYTVEIDQAPVNVFRVTCSAPGGQMLTLNVRTNLTVNNN
                     SSSDADWSFGPKGNVAINIHSNAELDAYCQILGAGESNNYTVLPGGTLRYGRTPSKRF
                     RVYGSLTVQGSVIAGNRAGELIYANPLILDGGYLFAKALSSPPELVTNNASLSYSLMR
                     IAGMTVTMRDGTVTNRADDALHIGNAASGNASAFLNLSGGLWWQEGPAYICERPNGYL
                     DLSGTAQFVSTNNVWVGSNNTGTQPFLPGYSGFVTVHSGNFCVTNSDGSARLYLGNIT
                     PGYLTCAGGTSSVDRLVLSNRGNVLGADAVGRITMAGGRLDILQSLSATNGSYSVLAC
                     STGTLNVANADVANDSALVIGDGKGEATLGLLSGTHRFADGLVITNNATLSAGGVGNR
                     ASAEVTGNLALAEGATLHCDFQDNAGDSLSLAGKLTLPTQAAVTLTSLDGTLPQHVVL
                     VAAEALDGAVDLSGWTVASIGEIGYRVAVEGASLVLLKKPKGMVISVL"
     gene            2406..5792
                     /gene="recC"
                     /locus_tag="PLZ74_03640"
     CDS             2406..5792
                     /gene="recC"
                     /locus_tag="PLZ74_03640"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOR97447.1"
                     /translation="MSFTLYHGNRLDQLAAALADLILQPPAGGAVRDPLRQETIVVPN
                     QGLARWLALEIAARRKICPPLDLPYPGAFLYRDLFDPMMGRTSRAVQPDLPFDPATME
                     WRLFALLEQLEPETAFARVHAFTRGDPLRRYQLAGRLASLYDRYMTYRPDLLAAWEQG
                     DEPLTGEDAAWQARLWQELIALDRGSVRHFSGLYRRFIELTRHPDRQPDFLAPLLARR
                     RIAYFGVSSLPPAHLDILIRLARFDDFEIHFFTPNPCCEEWSDARSLKTQIRDRLTLV
                     QTIGPQLAPAYEIRANPLLGSLGRSGREFFSLLLAYDEIHDERLFVEPDAAEGTVLGT
                     LQRGILRNTPLEPRQPVAPSDRSLVIHACHAPLREVEALHGHLLALFRDEPGLAPREI
                     SVYTPDIETFAPYIEAVFGKVPPGTPGHIPYTIADKSLLHTVDECRAWLALLTAVTGR
                     FKASEVLGLLQHAALRQRFNFVETDMPCLGSLLRAAHLSWGLDAAFRARQGASPAYAN
                     TWRFALDRLLFGAAMQDPTDEAPPLNLTEGKCIPLTEAEGHASLIGRLASFLDALAEL
                     HEFCLSRPTRPCGAWHAYLNAALERFFPTDERASYGVLLLRQTLDQFARYIEHAGCER
                     LAVPFSVIRAWLTAQLDGTPGSERFVTGRVTFSRFQPMRNVPARVVCLLGLSDKAFPR
                     NAPQLSFDLMDRRENRCIGDRRPRDEDRYAFLETLLAARERLIITYTGQSNKDNKPLP
                     PSVLVSELLDAIDAAFTFPSRDGVPQTASTALIVRHPLHPFSPAYFTRDKPDPRLRAF
                     DATAFRIACRLAGATGDASPQPEAQVVFPSAESAATDAPLQLEELIAFFLSPCKAFYT
                     RRLNAALTERDDELPDDLEALEPDTLVQYQIRQELTAAMLHGDGELAINRLRDRWQTD
                     GRLPVDADRHMAEARDRIDVVIQKVNALDLGEPLAPQPFDLDLPNGFRLQGTLNALRA
                     GNAQLFTRPAKEKPKDIVTAWITHLAACAVKPGVTTWGVFEGTKTIVTRYPPLESDAA
                     RRILAALAAHHAEGMTRPLCFDPAIGHKLVEGKPLTETDWTGSSFPSDPGVDIYLQHA
                     FGATLPEAGSEPLRTLERVARTLFGEMRKTPVKIQRGGRS"
     gene            5789..9013
                     /locus_tag="PLZ74_03645"
     CDS             5789..9013
                     /locus_tag="PLZ74_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOR97448.1"
                     /translation="MIPFDLLTTTLAPGTRFLIEANAGTGKTFQIQHLYLRLILEQNL
                     EPSRILVVTFTEAATAELRDRIRQCLATALAKLGEVEPENPPSLIDAILKQAAAQRTP
                     DALRVSLQLALAAFDEAAIFTIHGFCKRTIDHHAFESQVMFDCELIPSASELLGDLVA
                     DFFRRERHAKAEPFVSLHDLLNHAHLVMSHLGEARIEADGVTDPRLDVFARLRDHLHD
                     PEDGLTARMRRRQQMGYDDLLRILRDALAAGPDCPLAHVLRATYAAALIDEFQDTDPV
                     QYEIFKTVFAHPDSRLFLIGDPKQAIYGFRGGDLYTYLQARDEVAPSARFTLTRNFRS
                     ARELIEAVNSIFAPPGAFGEQQLGHPPSESGREPERTLLRDGHRDPTPFELVRFTGDH
                     TGGAVASSSLETPITRDCAARIATLLTDGRTMFQKPDQTLIPVTPGDIAVLTVTNSQA
                     EALQQRLRLHGVPAVIYKAGNIYASADAGNLCRLLGAMAEPNRVPRIKAALLTPHGGM
                     TVRDLLAAEKDPDAAADLERRIGDFAECGRLWREKGVVPALTLLLARLNSLERLANDP
                     ACERRLTNLRHLTELLHQTERAARLAPDALLRELKRQIADPDTDDETCEQRMESDRRA
                     VTLMTIHKSKGLQFPIVFVPYLITHDIRKGMEKGWAVHAPDAQGVMRLVLPVGDAARR
                     RFEEQRLHETLAEHLRLLYVAVTRAENRCVLMTGTAITYKKTPIRSAVAHLTRLAEPM
                     STGGTPFSLEEVTTETLDRPHAEPRHTPPDTHGDLVPPASPPLPPDDWAVLSYSGMTL
                     HDAPPPHLKPDAGTDEVADPLLSAPDRDRAGDVLPGGKTTGLCLHAILDQLDYTHVTP
                     AWQPAPDDLALIDRHAAFHGLYTSDSPLAAARRARIVDLLAQTLTRALPTAPADPGAN
                     APMSGGIPLSAVPFADTRREWEFFFRVPRAIDLTRFQELGLSFKPDSGTRRGFMTGSI
                     DLVFSCEGRTGFVDWKTDMLPDYTPATLAAAMTERNYLFQAILYAVALHTHLAQCLGA
                     AYDYERHFGGGHYVFIRGVDARNGLFPFRPPLAELEAWSRLLTH"
     gene            complement(9003..10664)
                     /locus_tag="PLZ74_03650"
     CDS             complement(9003..10664)
                     /locus_tag="PLZ74_03650"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOR97449.1"
                     /translation="MKRKTYVIGHRNPDTDSIVSAIAYAELKRQKGWTECFAARAGKL
                     TPQTEYILGRFNIPAPEFVPDLVPKVGYFLTGGAVTVGESEAFWDALETMEREKVSIL
                     PVVDAQGRYRAVLHHNAFASNITRKINPHRKAIIPTSMELLQKTLQAQPIVTFAAEEV
                     VRSRILVAGSSFESFREHLENEIPANALVLTGDREDVQRFSIESGARAVIITNGMPLA
                     KGLRELAEARRVTVMISPYDTSSTAFLILYSTPVMTVADEDAMPIAANSYVRTVREQL
                     AAAPSHCLPVVDDAGIVVGIFSESDLIAEPNIDIIMVDHNEMGQAVEGLENYRILEII
                     DHHRLGNFSTRYPITFINRVVGATSTIVAGMYIEQKAVLTPGVAALLLAGILTDTLML
                     RSATTTETDREMAEYLAGLVNLDIESFGHEIFSNARKVADLPVVDILGMDSKAYAACG
                     QRFAVSQVEVNAADDLLVRKAELLAALRQRRTGEGLFFTALMITDVTTLSSILLVEGD
                     ETFIKKIAFPRLEPGVFRCRDILSRKKQLIPLLIEIMETVLGVNA"
     gene            10800..12275
                     /locus_tag="PLZ74_03655"
     CDS             10800..12275
                     /locus_tag="PLZ74_03655"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOR97450.1"
                     /translation="MHLPPALREKLAALPDKPGCYLMRDRTGTIIYVGKAVSLRRRVQ
                     SYFRPSTLRAAPPKLRGMLRSVADLDILTVRNEAEALLTESQLIKQYRPRFNVVLRDD
                     KRYLALRADPREPVPRLATCRIVRADSAMYFGPFPSATVVRTVLDFTEKRYGLRKCTP
                     LEPDAETYRHCINDIVRFCSAPCIGRISREAYRERFDEACAFLRGERPAVIEEVRTRM
                     DEASQKRDFEKAASLRDTWLALREMVRRRAITVAAPERHAEDARDGILDLQRRLGLPH
                     VPARIEGFDISNLFGTHSVASMVVAVDGLPERRQYRRFRIRTVEGADDPRAIAEVIRR
                     HYARMRGEHRPLPGLVLIDGGIPQLRAAREVLDTLGLGRVPTAGLAKRMEEIVLDNGA
                     PPLRLPYDSPALRVLIRLRDEAHRFALDYHRRLRNRLIRESALDEIPGIGPAKKAALL
                     RRFGSVYRLARASAAEVADVPGISPGLAEAILRAVDTRKTP"
     gene            complement(12459..13307)
                     /locus_tag="PLZ74_03660"
     CDS             complement(12459..13307)
                     /locus_tag="PLZ74_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOR97451.1"
                     /translation="MRKGPLNSYSLGWSLALHGVLLVAAIVLPLLSFLRPKPLQIPID
                     FTVVLEENLVEPNVPDTPPAPPTEPEAAPEPDPAPPKPAPLPDPPKDAVVVEKKKEPE
                     KKKEPEKKKEPEKKKEPEQKKPAPFKKGKRIEAPKQPQQPRFEDLYKPYDPSKPQSRK
                     PKTDKMLTRAEIEKALRDGARPGSRNVIPQDEIARCVTLVRRAMYEAWEQPGSGDAGS
                     RPALLDIRLDQAGRVVSYRIRQSSGSPYFDQTVLKAAANAAPIRGLTPAFLKRYETLT
                     VEFKLE"
     gene            complement(13315..13725)
                     /locus_tag="PLZ74_03665"
     CDS             complement(13315..13725)
                     /locus_tag="PLZ74_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOR97452.1"
                     /translation="MALRRLGRRKPSKVINNIDMTSLIDLTFLLLITFIITMPAIEQG
                     ISIRLPQGKADELPVKKSNVVTLDVEGKAYLNNRVISLEDLERTLGNMAAEDPEVAVL
                     VRGDERLEYGKIMRVMKILYKVKITRMALVTLSE"
     gene            complement(13725..14471)
                     /locus_tag="PLZ74_03670"
     CDS             complement(13725..14471)
                     /locus_tag="PLZ74_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOR97453.1"
                     /translation="MFGTGMISISLPLASLIGGFMESNLMGKGIVLVQLASSIVAAAI
                     IIGKNRELSSVMRNSKRFMREFLLGHDVLDYYLLRKKGSFSALELIYERTCERMVKLL
                     DPDMRRSVIGRQRDAAGAALSAREIELVRSTCEHTVQDEELRIEYGMGLLATIVTASP
                     MLGLLGTVWGVLDAFADMGSKGTVLLSVIAPSISSALVTTVVGLLVAIPSAICYNNLA
                     AKVRQITTDMVGFADELMGRIACEFQGRTD"
     gene            complement(14571..15380)
                     /locus_tag="PLZ74_03675"
     CDS             complement(14571..15380)
                     /locus_tag="PLZ74_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOR97454.1"
                     /translation="MLATFHNHSVWSDGADTLEALYEQAGRLGVSLLGMSEHFCAYPD
                     GAAVAGYLPADRIDAYLSTLREVGQTGPVEVVAGLEFDWTEAGAEWVRPYAEGLPLDY
                     RIGSVHYVAGEGIDIDASFWMSKTQEEIDAVYAGYWRLIRGMAESGLFDIVGHLDLPK
                     KFGFVSGPSLRGLEDEALDAIAASGMTVEFNTAGFDKPCAEGYPSADLLKRCRQRGIP
                     VTLSSDAHRAAHVIRGFERGAAVLAAAGYDRVARFRGREQWFEGLDDAVVR"
     gene            complement(15420..>17773)
                     /locus_tag="PLZ74_03680"
     CDS             complement(15420..>17773)
                     /locus_tag="PLZ74_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97455.1"
                     /translation="RCFFGPIKRPPRYRDELMDDVDYYPEAYLNRLAHEGINGLWLTV
                     TFRDLCRTEITPGFGEQADRRFAKLRRTVERCRRYGIRIYLFAIEPAAWDADDPVLKN
                     HPELGGAKAGGKLMFCPSSETAQRYLYEAAKGIFSAVPHLGGLINITHGERYTSCQSV
                     VVATSNGRTTCPVCAKKEPWEIFQATLEPLSRGMREANPHAELIAWFYMPQDADPGKW
                     VYAIPQHGLSNVVYQFNFESGVTKTDFGRVRLGGDYWISTPGPSARFERLADLARESG
                     VAFSAKIQTGCSHEVATVPFVPVPGLLYRKFRAMRELGVSRVMLCWYFGNYPGIMNKA
                     AGELAFEPFPETEQAFVSELARAEWGRARAGTVAQAWERFAEGYGHYPLVTPFQYWGP
                     MHDGVVWPLLLLPQDAPLAPTWLIAANRGERVGDPFPPSGDRIAEALGGAYTLQEAIE
                     ACRAMSVMWDQGVGLLRTLERDSGLPKARRQDIGVSRALGLQFRSGLNILRFYDLRER
                     LARETVKARQLALLSEMRGIVEKEAAGGVELKELCRADSRIGFHSEAEGYKYFPAKIA
                     WRGEQLRRVLDTEMPALERSFAEGKDVFAAYAGRQPEGPCARSVFRADIARQIEEGSA
                     WLKEQDWQMDSTTNTFRWTCRHDRDALYLLFTCDNTPSAKRADGTVSLILEPRRLWPS
                     MTCRVSADGKAHSSPDVMGAHYAFQSGVRREEGGWSGWMRIPFVSIGIPSERPAPVRI
                     NVVRVAAGSRDEQAWITRRPWPYRLRVSGGNPADLGWLLFDGP"
BASE COUNT         3318 a         6260 c         4908 g         3287 t
ORIGIN      
        1 caccgttgac gttgaacgtc aggggggtta agtccgatgc cgatttctcg atgctcagga
       61 acgtgtttgt cagaaacgcg cccggcggcg gcgtgtagtt gatcgtaccc gtgacgtttg
      121 atgcggcgac aatgtacacg cccacattcg ctgtgccgtc agtcagataa cggctgcccg
      181 gaacctccgg gggatcgatc ggcgtcccgt caaccgtcag gctccagttg gccgcgttga
      241 accaatcccc gtctccggca ctcccgcacc acacatagat gttcgtcgtc gtggcgtgaa
      301 gcgtgagaac gaacatcgac aggaccaacc cgaacatcca ttctctcaat ctcattttca
      361 tgatttgatc ctcctgtcac gcatcgcgac cctaaccatc cgcagtgcca cgttacaaca
      421 cgctgatcac catacccttc ggtttcttga gcagcaccag cgaggcccct tccaccgcca
      481 cgcggtaccc gatctcgccg attgacgcga ccgtccaccc cgaaaggtcc accgctccgt
      541 caagcgcctc ggcggccacc aaaacaacgt gttgcggcag agtgccgtcc aacgaggtca
      601 gcgtgactgc ggcttgcgtc ggcaaggtca gcttgccggc caaggaaagg ctgtcacccg
      661 cgttgtcctg aaaatcgcag tgcagcgtcg cgccttcggc cagcgcgaga ttccccgtga
      721 cctctgcgga agcccggttc ccgaccccgc cggcactgag cgtggcgttg ttcgtgatca
      781 ccagaccgtc ggcaaagcgg tgcgtgccgg agagcaagcc gagcgtagcc tcgcctttcc
      841 cgtcaccgat gaccagcgcg ctgtcgttgg cgacgtccgc gttggccaca ttgagcgtgc
      901 ccgtcgaaca ggccagcacc gagtaactgc cgtttgtcgc ggacagcgac tgcaggatgt
      961 ccagccgtcc gcccgccatc gtgatccgcc cgaccgcgtc ggcccctaaa acgtttcccc
     1021 gattagacaa cacgagccga tccaccgacg aagtgccgcc cgcgcacgtc aaatagccgg
     1081 gcgtgatgtt gccgagatac agccttgcac ttccgtcgga attggtcacg cagaagttgc
     1141 ctgaatgaac cgtcacgaac ccgctgtagc cgggcaggaa cggttgggtg cccgtgttgt
     1201 tggatcccac ccacacgttg ttggtggaga cgaattgggc cgtacctgac agatccagat
     1261 acccgttcgg ccgctcgcag atgtaggcgg gcccctcctg ccaccagagc ccccccgaca
     1321 aattcagaaa cgccgacgca tttccggagg cggcgtttcc gatgtgcagc gcgtcgtccg
     1381 cgcggttcgt gaccgtcccg tcccgcatcg ttaccgtcat tcccgcaatc ctcattaacg
     1441 aataactcaa cgacgcattg ttcgtcacga gctccggagg tgaactgagg gcttttgcaa
     1501 acagataccc cccatccaat atgagcggat tcgcgtaaat cagttcgcct gcgcgattac
     1561 cggcgatcac gcttccctga accgtcaggc tgccatacac ccggaagcgc ttactgggag
     1621 tcctgccgta tctcaatgtc ccgccgggaa gaacggtata gttgttgctt tcgcccgcgc
     1681 ccagaatctg gcagtacgca tccaactcgg cgtttgaatg aatgttgatc gccacattcc
     1741 ctttgggccc gaacgaccag tcggcatcag aagaactgtt attgttcaca gtcagattgg
     1801 tccggacatt cagcgtgagc atctggcccc cgggcgcgga gcatgtcacc cggaacacgt
     1861 tgaccggggc ctggtcgatt tcaacggtat atccgtttcc cgttgtggcg gcatttgtga
     1921 tgattaccgt gtcctccgct ccgggaacgc ggtccaagct ccaattcgct ggctcgttcc
     1981 agttgcccgt gcccgaggtc gcgatccaat aattcgtctc cgccgccgca gaaaaaacga
     2041 tccaaccgac agccacgcct gtcatcacaa caactggctt catactcatc cctcccctac
     2101 ttcagagcaa cgcacctcag cgaaacagcc catagtcgat aaatgatatg gaattacata
     2161 tgattgtact tgtgtttgac acgttccgtc aacgatgatc atgcgaaatc gaacgatctt
     2221 tttcggaacc gcatcgttgc gtttgtgatg atgccgcaat ataatcgata ttcttgatta
     2281 aggttctcgt aacagacgat gaaacggaat cctcagaaaa aatgcctcat gacaaggctg
     2341 cgcgcgcaga accgcacgcc ggaggcgacg catgtcacgg atgggaggat ggggagggcc
     2401 gccctatgag tttcacgctg tatcacggca accggctgga tcaactcgcg gcggcactcg
     2461 cggacctcat cctccaaccg cccgcaggcg gtgcggtgcg ggatccgttg cggcaggaaa
     2521 caatcgtggt ccccaatcag ggcctggccc gctggctcgc cctcgaaatc gccgcccggc
     2581 ggaaaatctg tccgccgctc gacctcccct atcccggtgc attcctctat cgcgacctgt
     2641 ttgatccaat gatgggacga acctcccgcg ctgttcaacc ggacctgcct ttcgatcccg
     2701 ccacaatgga gtggcgcctg ttcgcactgc tcgaacaact cgaaccggaa accgcgttcg
     2761 cgcgcgtcca cgccttcacc cgcggcgacc cgctgcgccg ttaccaactc gccggtcggc
     2821 tggcatcgct ctacgaccgc tacatgacct accggcccga tttgttggcc gcctgggaac
     2881 aaggtgacga accgctgacc ggtgaagacg ccgcatggca ggcccgcctc tggcaggaac
     2941 tcatcgcgct cgaccgcgga agcgtgcgtc acttctccgg actctaccgc cgcttcatcg
     3001 agctcacccg gcaccccgac cggcagcccg acttcctcgc cccgctgctg gcccggcgcc
     3061 gcatcgcgta tttcggcgtc tcctccctgc cgcccgctca tctggatatc ctgatccgtt
     3121 tggcgcgttt cgacgacttc gagatccact tcttcacccc caacccctgc tgcgaggagt
     3181 ggagtgacgc acgcagtttg aaaactcaga tccgcgaccg gctcactctg gttcagacaa
     3241 tcggtcccca actcgccccc gcctatgaga tccgcgccaa cccgctgctt ggctcgctcg
     3301 gacgcagcgg acgcgaattc ttctcgttgc tcttggccta tgacgaaatc cacgacgaac
     3361 gcctgtttgt ggagccggat gccgctgaag gaacggtgct cggaaccctc cagcgcggga
     3421 tcctccggaa cacgcccctc gaaccgcggc agcctgttgc cccctctgac cgctccctgg
     3481 tgatccatgc ctgtcacgcg ccgctgcgcg aggtcgaggc cctgcacggc cacctgctcg
     3541 ccttgttccg cgatgaaccc ggcctcgcgc cgcgcgaaat cagtgtctac acgccggaca
     3601 tcgaaacctt cgccccatac atcgaagccg tcttcggaaa agttccgccc ggcacacccg
     3661 gccatatccc ctacaccatc gcggacaagt cgctgctgca cacggtggac gagtgtcggg
     3721 catggctcgc cctgctcacc gccgttaccg gacgcttcaa agcctcggag gtgctcggac
     3781 tgctccagca tgccgcactc cgccaacggt tcaacttcgt cgagaccgac atgccgtgcc
     3841 tcggcagctt gctcagagcc gcgcaccttt cctggggcct cgacgcggct tttcgtgcac
     3901 ggcaaggggc cagtccggcc tatgccaaca cgtggcgttt tgccctcgac cgccttcttt
     3961 tcggtgccgc catgcaggac ccgactgatg aagccccgcc tctgaacctg acggaaggaa
     4021 agtgtatccc attgaccgaa gccgaaggtc acgcctcgct gatcggccgt ctcgcatcgt
     4081 tcctcgatgc cctcgcggaa ctgcatgaat tctgcctctc ccgcccgaca cgtccctgcg
     4141 gtgcgtggca cgcctacctg aacgcggcac ttgagcggtt cttccccacg gacgagcgcg
     4201 cgtcttacgg cgtgctgctg ctgcggcaaa ccctcgatca gttcgcccgc tacatcgaac
     4261 acgcgggctg tgagcggctt gcggtcccct tctctgtgat acgcgcctgg ctcaccgctc
     4321 agctcgacgg cacaccgggc agcgaacgct ttgtcaccgg ccgcgtgacc ttctcgcgat
     4381 tccaaccgat gcgcaacgtc cctgcccgcg tggtctgcct gctcggactc agtgacaagg
     4441 ccttcccccg caacgcgccg cagttgagtt tcgatctgat ggaccgacgc gaaaaccgtt
     4501 gtatcggcga ccgtcggcca cgcgacgagg accggtacgc ctttctggaa accctgctcg
     4561 ccgcgcgcga acgtctgatc atcacctata ccggacagag caacaaagac aacaagccgt
     4621 tgccgccctc ggtgctggtc agcgaactgc tcgatgccat cgacgccgcc ttcacctttc
     4681 cgtctcgcga cggtgtcccc caaaccgcct ccacagccct catcgtgcgc cacccgctcc
     4741 acccgttcag cccggcctac ttcacacgcg acaagcccga cccgcgcctc cgcgcgttcg
     4801 atgccaccgc tttccgcatc gcctgtcgcc tggccggcgc gaccggagac gcatcaccac
     4861 agcccgaggc acaggttgtt tttccgtcag cggaatccgc cgcgaccgac gcgccgctcc
     4921 aacttgaaga actgatcgct tttttcctct cgccatgcaa agccttctac acgcggcgtc
     4981 tcaacgccgc cctgacggaa cgcgacgacg aattgccgga tgacctcgaa gcgcttgagc
     5041 ctgacacgct cgtccaatac cagatccggc aggaattgac cgcagcgatg ttgcacggcg
     5101 acggtgagct tgccataaac cgtctgcgtg accgttggca gactgacggc aggctaccgg
     5161 ttgacgccga ccgacacatg gcagaggccc gtgaccggat agacgtggtg atccaaaaag
     5221 tcaacgcgct tgatctcggc gagccgctcg ccccccagcc gttcgatctc gatctgccca
     5281 acgggttccg gctgcagggc accctgaacg cgttgcgggc cggcaacgcc cagcttttta
     5341 cgcggcctgc caaagaaaaa ccaaaggata tcgtaacggc ctggatcacc cacctcgccg
     5401 cctgcgccgt caaaccgggc gtcaccacct ggggcgtctt tgaaggaacc aaaacgatcg
     5461 taacccgtta tccacccctg gagtctgacg cggcgcgccg tatcctcgcc gccctagccg
     5521 cacaccacgc agaaggcatg acgcggccgc tgtgtttcga tcccgcgatc ggccacaaac
     5581 ttgtcgaagg gaagccgctc accgaaaccg actggacggg atcgtcgttc ccatccgatc
     5641 ccggtgtcga catctacctg caacacgcct tcggcgcgac gctccccgaa gccggttcgg
     5701 aacccctgcg cacactggaa cgggtggccc gcacactctt tggtgaaatg cggaaaacac
     5761 ccgtaaaaat ccagcgggga gggcgctcat gatcccgttc gacctcttga ccaccacgct
     5821 cgcccccggt acgcgcttcc tgatcgaagc caacgctggc accggaaaaa ccttccaaat
     5881 ccaacacctt tacctgcgcc tgatcctcga gcaaaatctc gaaccgtcgc gcatcctggt
     5941 cgtcaccttt accgaagccg cgaccgctga actgcgcgac cgcatccgcc aatgcctcgc
     6001 aacggcgctg gcgaagctcg gagaggtaga gccggaaaac ccgccatcgc tgattgacgc
     6061 gattcttaag caggccgcgg cgcagcgcac acccgatgcg ctgcgcgtga gcctgcaact
     6121 cgctctggcc gcgtttgacg aggccgcgat cttcaccatc catgggtttt gcaaacgtac
     6181 gatcgaccac catgcctttg aaagtcaggt gatgttcgat tgcgaactca tcccctccgc
     6241 ttcggagctg ctcggcgatc tggtcgccga cttcttccgc cgcgaacggc acgccaaagc
     6301 cgagccattc gtttcactgc acgatcttct caaccacgcg caccttgtca tgagccacct
     6361 cggggaagca cggatcgagg ccgacggcgt gaccgatccc cgactcgatg ttttcgcccg
     6421 cttgcgcgat cacctgcacg accctgagga cggtctgacc gcgcgcatgc gccgccgaca
     6481 gcagatggga tacgacgatc tgctgcgcat cctgcgcgac gcgctggccg ctggcccgga
     6541 ttgtccgctg gcgcacgtgc tacgcgccac ctacgcggcc gcgctgatcg atgagtttca
     6601 agataccgac cccgtgcagt acgaaatctt caagacggtt ttcgcccacc ccgactcccg
     6661 actctttctg attggcgacc ccaaacaggc gatctacggt ttccgcggcg gagacctcta
     6721 cacctatctt caggctcgcg acgaggtcgc gccgtcggcg cgcttcaccc tgacgcgcaa
     6781 cttccgttcc gcccgcgaac tgatcgaggc cgtcaacagt atctttgcgc cgccgggcgc
     6841 ctttggcgaa caacagctcg gccacccgcc ttcggagagc ggtcgcgaac ccgagcgaac
     6901 cctgctgcgc gacggccacc gcgatccgac gccgttcgag ctcgtccgct tcaccgggga
     6961 ccacacgggc ggcgcggttg cctcctcctc gctggaaacg cccatcaccc gcgactgcgc
     7021 cgcacgcatc gccacgttgc tgacggacgg tcgcaccatg ttccaaaagc cggatcagac
     7081 gttgatcccg gtcacgccag gcgacatcgc cgttctgact gtcaccaaca gccaagccga
     7141 agcgcttcag cagcggctgc gccttcacgg cgtcccggct gtgatctaca aagccggcaa
     7201 catttacgcc tccgcagacg cgggcaacct ctgccgtctg ctcggcgcca tggctgaacc
     7261 caaccgcgtc ccgcgcatca aggccgccct gctgacgcca cacggcggca tgaccgtccg
     7321 cgatctgctc gcggcggaga aggacccgga cgccgccgcc gacttggagc gtcgcatcgg
     7381 tgattttgcc gaatgcggcc gcctctggag agaaaaaggt gtcgttcccg cgctcacgct
     7441 tttgctcgcc cgtctcaaca gcctcgaacg actcgccaat gatcccgctt gcgagcgccg
     7501 cctcaccaac ctgcgacacc tcaccgaatt gctgcatcag accgaacgcg ccgcccgtct
     7561 cgcgccggat gcgctgttgc gcgagctgaa acgccagatc gcggatcccg acaccgacga
     7621 cgaaacctgc gagcagcgca tggagagcga ccgccgcgcc gtgacgctca tgaccatcca
     7681 caagagcaag gggttacaat tcccgatcgt cttcgtaccc tacctgatca cgcacgacat
     7741 ccgcaaaggc atggagaagg ggtgggccgt ccacgcaccg gacgcgcagg gtgtcatgcg
     7801 cctcgtcctg ccggtcggcg acgcggcccg ccgccggttc gaggagcagc ggctgcacga
     7861 gaccctcgcc gaacacctgc gcctgctcta cgtcgccgtg acccgcgccg aaaaccgctg
     7921 tgttctgatg accggcaccg cgatcaccta caagaaaacc ccgatccgaa gcgcggtggc
     7981 gcatctgacc cgcctagcgg aacccatgtc aaccggcggc acgccgtttt cgttggaaga
     8041 ggtcacaacc gaaaccctcg accgtccgca cgccgagccg cgccacacgc cgccggacac
     8101 gcacggcgac cttgtcccgc ccgcaagccc gccgctgccg cccgatgact gggccgtact
     8161 cagttactcc ggcatgaccc tccacgacgc cccgccgccg cacctcaaac cggacgctgg
     8221 caccgatgag gtcgctgacc ccctcttgtc cgcaccggac cgggaccgtg ccggggatgt
     8281 tttgcccggc ggcaaaacca ccggcctctg tctgcacgcg atcctggatc aactcgacta
     8341 cacgcacgtc accccggcgt ggcaacccgc accggacgac ctcgcgctga tcgaccgcca
     8401 cgccgccttc cacggactct acacatccga ctccccgctc gcggccgcac gccgcgcccg
     8461 tatcgtggac ctcctcgccc aaaccctgac ccgcgccctg ccgaccgctc ccgccgatcc
     8521 cggcgcaaat gccccaatga gcggcggcat cccgctctcc gcggttccct ttgccgacac
     8581 gcggcgcgaa tgggagttct ttttccgcgt gccgcgcgcg atcgacctca cgcgatttca
     8641 ggagttgggc ctgtccttca aaccggacag cggcacgcgg cggggcttca tgaccggatc
     8701 gatcgatctg gttttctcct gcgaaggccg cactggtttc gtggactgga agaccgacat
     8761 gctccccgac tacacgcccg cgacgctagc cgccgccatg accgagcgca actatctctt
     8821 tcaggcgatc ctctacgccg tggccctcca cacgcatctc gcgcagtgtc tgggtgcggc
     8881 ctacgactac gagcgccatt tcggcggcgg ccactacgtc tttatacgcg gggtggatgc
     8941 ccgcaacgga ctcttcccct tccgtccccc gctcgctgaa ctcgaagcgt ggtcccgcct
     9001 gcttacgcat tgacgccgag gaccgtctcc atgatttcaa tcaggagcgg aataagctgt
     9061 tttttacgcg agaggatatc gcggcaacgg aagaccccgg gttccagacg cggaaaggcg
     9121 atctttttga taaaggtttc gtcgccctcg accagcagga tcgagctgag cgtcgtgaca
     9181 tcggtgatca tcagcgccgt aaagaaaagc ccctcccccg tgcggcgctg ccgcaacgcc
     9241 gccagcagtt cggctttgcg caccagcaga tcgtcggccg cgttgacctc cacctgactg
     9301 acggcaaaac gctgtccgca cgccgcgtac gctttggagt ccatgccgag gatgtccaca
     9361 accggcagat ccgccacttt acgggcgttg ctgaaaatct catgtccgaa agactcgatg
     9421 tccagattca ccaatccagc cagatattca gccatctcgc gatccgtctc ggtggtggtc
     9481 gccgaccgca gcatcagggt gtcggtcagg atccccgcca acagcaacgc cgccacgccg
     9541 ggggtcaaca ccgccttctg ctctatgtac atccccgcga cgatggtcga ggtcgcgcca
     9601 accacgcggt tgatgaacgt gatcggatag cgggtggaga agttgcccag gcggtgatgg
     9661 tcgatgatct cgaggatgcg gtagttttcg agaccctcga ccgcctgccc catctcattg
     9721 tggtcgacca taatgatgtc gatgttcggt tcggcgatca agtcgctctc ggagaagatg
     9781 ccgaccacga tgcccgcatc gtcgaccacc ggcaggcaat gcgagggcgc ggcggcgagc
     9841 tgttcgcgca cggtgcgcac atagctgttg gccgcgatcg gcatggcgtc ctcgtcagcc
     9901 acggtcatca ccggcgtgga atagaggatc agaaaggcgg tcgaggaagt gtcgtacggc
     9961 gagatcatga ccgtaacgcg gcgggcttca gccaactcgc gcaacccctt ggccagcggc
    10021 atgccgttgg tgatgatcac ggcacgcgca ccgctctcga tactgaagcg ctgcacgtct
    10081 tcgcggtcac cggtgagcac cagcgcgttg gccgggattt cattttccag atgctcccga
    10141 aacgattcga atgacgatcc cgccaccaga atacgcgacc gcaccacctc ctctgcggcg
    10201 aaagtcacga tcggctgtgc ctgaagcgtc ttctgcagca gttccatgct ggtggggatg
    10261 atcgccttgc ggtggggatt gatctttctg gtgatgttgc tggcaaaggc gttgtggtga
    10321 agcacagccc ggtaacggcc ttgcgcatcc acaaccggca gaatgctcac cttctcccgc
    10381 tccatcgtct ccaaggcatc ccagaaggcc tccgattcac cgaccgtcac cgcgccgccc
    10441 gtgagaaaat accccacctt gggcaccaga tccggcacga actccggagc cgggatgttg
    10501 aaccggccga ggatgtactc cgtttgcggc gtcagtttgc ccgcgcgtgc ggcaaagcac
    10561 tcggtccagc ccttctggcg tttcaactcc gcgtaggcga tcgccgagac gattgagtcg
    10621 gtgtcaggat tacggtgtcc gatcacatag gtttttctct tcatgcgagg cagaatcata
    10681 ggctatcgct tccagaaaag ccagaggcaa tccgaattcc ccttgccgga ggtttctccg
    10741 catgccaaac taacgccatc gacatcgtga acccggtttt tgtgtcacct tggagggtta
    10801 tgcatctccc ccccgcactt cgagaaaaac tcgcggccct gccggacaaa cccggctgct
    10861 atttgatgcg cgaccgcacg ggcaccatca tctatgtggg caaagcggtc tcgctgcgcc
    10921 gccgcgtgca gagttatttc cgcccctcca ccctacgcgc cgcgcccccc aaactgcgcg
    10981 gcatgctccg cagtgtcgcg gatctggaca tcctcaccgt acgcaacgag gccgaagcgc
    11041 tcctcaccga aagccagctc atcaaacagt accgtccccg tttcaatgtc gtgctgcgtg
    11101 acgacaaacg gtatctcgcg ttgcgcgcag acccgcgtga gccggtcccc cggctcgcca
    11161 cctgccggat cgtgcgggct gactccgcaa tgtacttcgg cccctttcct tctgccaccg
    11221 tcgtccgcac ggtgctcgat ttcacggaaa aacgttacgg tctccgcaaa tgtaccccgt
    11281 tggaaccgga tgccgaaacg taccgccact gcatcaacga tatcgtgcgt ttctgctccg
    11341 ccccctgtat cgggcgcatc tcccgcgaag cgtaccggga gcgtttcgat gaggcctgcg
    11401 cattcctgcg cggcgaacgg cccgccgtga ttgaggaggt ccggaccaga atggacgaag
    11461 cctcgcaaaa acgcgatttt gaaaaggccg cttcgctgcg cgacacatgg ttggcactgc
    11521 gcgagatggt ccgtcggcgc gcgatcacgg tcgccgcgcc cgaacggcac gccgaggatg
    11581 cccgtgacgg cattctggac ttgcagcgcc gactcggcct gccgcacgtg cccgcacgca
    11641 tcgaaggctt cgacatctct aacctgttcg gcacgcattc ggtcgccagc atggtggtgg
    11701 cagtggacgg cctgcccgag cggcgtcagt accggcgctt ccgcatccgc accgtggagg
    11761 gtgcggacga tccgcgagcc atagccgaag tcatccgccg ccactatgcc cgcatgcgcg
    11821 gggaacaccg ccccctgccc gggctggtcc tcatcgacgg cggcatcccc caactccgcg
    11881 ccgcgcggga ggttctcgat actctcgggc tcggtcgcgt gcccacggcc ggactggcta
    11941 aaagaatgga agagatcgtc ctcgacaacg gtgcgccccc gctccggcta ccgtatgatt
    12001 ccccggccct gcgcgtgctc atccgtttgc gtgacgaggc ccaccgtttc gccctcgact
    12061 atcaccgccg cctgcgcaac cggctcatcc gcgaatccgc gctggatgag attcccggca
    12121 tcggccccgc caaaaaagct gccctgctgc gccggttcgg ctcggtttac cgtctcgccc
    12181 gcgcttcggc tgccgaggtg gccgacgttc ccggcatctc gccgggcttg gcggaagcca
    12241 tcctccgtgc cgtggacacg cggaaaacgc cgtgaacgtt accgcgcagg cacgtcggtt
    12301 acgaatgtga aggacgtccc ctgcgcgtga acgcgtcgca gcccaaaccc cgcgaagaac
    12361 ggggattccg cagcggcggc aagcgtcgcg aacgcgggta ggcgcgggaa ccgaagcggc
    12421 acatgccaca gcggcacacg caatgatgcc gccgtttttt actccagttt gaattccacc
    12481 gtcagcgtct cgtaccgctt gagaaaagcc ggcgtcagcc cccggatcgg cgcggcgttg
    12541 gcggcggcct tgagtaccgt ctgatcgaaa tacgggctgc ctgaagattg ccggatgcgg
    12601 tagctcacca cacgccctgc ctgatccaga cgaatgtcca gcagcgcggg acgtgagcca
    12661 gcgtcgcccg agccgggctg ctcccacgct tcgtacatcg cgcgccgtac cagcgtgacg
    12721 caacgcgcga tctcgtcttg cggaatgaca ttccgactgc ccggccgcgc gccgtcacgt
    12781 agcgccttct cgatctctgc gcgggtcaac attttgtcgg tcttcggttt cctactctgc
    12841 ggcttactcg ggtcatacgg cttgtaaaga tcttcaaacc tgggttgctg aggttgctta
    12901 ggcgcttcga tgcgtttgcc ttttttgaac ggcgcgggtt tcttctgctc cggctctttc
    12961 tttttttccg gctctttctt cttctcgggc tctttctttt tttccggctc tttcttcttc
    13021 tcgaccacca ccgcatcttt cggcggatcg ggcagcggcg cgggtttcgg cggcgcggga
    13081 tccggttccg gcgcggcctc cggctccgtc ggaggagcgg gcggcgtgtc cggcacattc
    13141 ggttccacga gattctcctc cagcaccacc gtgaaatcga tcggaatctg tagcggtttc
    13201 ggccgcaaaa acgacagcaa gggcaacacg atggctgcca cgagcaacac cccgtgcagc
    13261 gccagcgacc agccgagcga atagctgttt aacggaccct tcctcacgct tgcctcactc
    13321 cgacagggtc accaatgcca tgcgcgtgat cttgacctta tacaggatct tcatcacccg
    13381 catgatcttt ccgtattcca gacgttcatc ccccctcacc aacaccgcca cttccggatc
    13441 ctctgccgcc atgttcccca atgtccgttc gagatcctcc agcgagatca cgcggttgtt
    13501 gaggtacgcc ttgccttcca cgtccagagt cacgacattg gatttcttga ccggcaactc
    13561 gtccgctttc ccctgaggca ggcggatgga aatcccctgt tcaatggccg gcatggtgat
    13621 gatgaacgtg atcagcagca ggaaggtcag gtcgatcagc gacgtcatgt cgatgttgtt
    13681 gatgaccttt gacggtttcc tgcgcccaag tcgacgcagg gccattagtc ggtcctccct
    13741 tggaactcgc aggcgatgcg gcccatcagt tcatccgcga acccgaccat atcggtcgtg
    13801 atttgccgca ccttcgctgc cagattgttg tagcaaatcg cactcgggat cgcgaccagc
    13861 agacccacca ccgtggtgac cagtgccgag gagatcgacg gcgcgatcac ggacagcagc
    13921 accgtcccct tggaccccat gtccgcgaac gcatccagta cgccccacac cgtgccgagc
    13981 agccccagca tcggcgaagc cgtcacaatc gtcgccaaca gccccatccc gtactcgatc
    14041 cgcaactcct catcctgcac ggtatgttcg caggtgctgc gcaccagttc gatctcgcgc
    14101 gccgaaaggg ccgcgccagc cgcgtcacgc tgccgtccga tcactgaacg gcgcatgtca
    14161 gggtccaaca gcttcaccat ccgttcacat gtgcgctcat agatcagttc cagcgccgag
    14221 aacgaacctt tttttcgcag caggtaataa tccagcacat cgtgtcccag cagaaactcg
    14281 cgcatgaagc gcttgctgtt gcgcatcaca ctgctcagct cccggttttt cccgatgatg
    14341 atcgccgccg ccacaatcga agaggccagc tggaccagca cgatcccctt gcccatcaga
    14401 ttggactcca tgaacccgcc gatcaacgaa gccagcggta agctgattga tatcattcct
    14461 gtcccgaaca tgcgtgtcgc ctcctgaaaa cactgaaagc agttaagact tagcatgcgt
    14521 cacccccgcc cggatgtcaa ccatccgtta tgccggatat tcgcaccccc tcaacggacc
    14581 accgcatcgt ccagaccctc gaaccactgt tcgcgcccgc ggaaccgggc gacacgatca
    14641 taccccgccg cagccagaac cgcggcgccc cgttcaaaac cccggatcac atgcgcggcc
    14701 cggtgcgcgt ccgacgagag cgtgaccggt atgccgcgct gccgacagcg tttcagcaga
    14761 tctgccgacg gatacccttc cgcgcacggc ttgtcgaacc ccgccgtatt gaactccact
    14821 gtcattccgg acgcggcaat cgcgtccaac gcctcgtctt ccaaaccgcg cagactcgga
    14881 ccggatacaa aaccgaattt cttgggcagg tccagatgcc cgacgatatc gaacagaccg
    14941 ctctccgcca tcccgcggat cagacgccaa tacccggcat acaccgcgtc gatctcctct
    15001 tgcgtcttgc tcatccagaa ggatgcgtcg atgtcgatcc cttcccccgc gacgtaatgc
    15061 acggacccga tgcggtaatc cagcggcagc ccctccgcat acggccggac ccactctgct
    15121 cccgcctccg tccagtcgaa ctccagtccc gcgacaacct ccaccggtcc ggtctgtccg
    15181 acttcgcgca gcgtgctcag atacgcgtcg atccggtcgg ccggcaggta tccggcaacc
    15241 gccgcgccat ccggataggc acaaaaatgt tcggacatgc ccagcaggga cacgccaagt
    15301 cgtccggcct gttcgtaaag tgcctccaag gtgtccgccc cgtcgctcca cacggaatgg
    15361 ttatgaaatg ttgccaacac gtctgcatcc ttttcttacg ctgccgtcag tttgtgtgat
    15421 caggggccgt caaagagcag ccacccgaga tccgccggat tgcctccgct caccctcagc
    15481 cgatagggcc agggacgccg cgtgatccag gcctgttcgt cgcggctgcc agccgcgacg
    15541 cgcaccacat tgatccgcac cggcgcaggt ctttccgacg ggatgccgat cgaaacgaaa
    15601 ggaatccgca tccatccgct ccagccgccc tcttcacgtc gaaccccgga ctggaacgcg
    15661 tagtgcgccc ccatgacatc gggagaggag tgcgccttcc cgtcggcgga tacccggcag
    15721 gtcatactcg gccagaggcg acgcggttcg aggatcaggc tcaccgttcc gtcagcacgc
    15781 ttcgcggaag gggtgttgtc gcatgtgaaa agcagataga gcgcatcgcg atcgtggcgg
    15841 caggtccagc ggaaggtgtt cgtcgtggaa tccatctgcc aatcctgctc cttcaaccac
    15901 gcggaaccct cttcgatttg ccgcgcaatg tcggcgcgaa acacactgcg cgcgcaaggc
    15961 ccctcgggct gcctgcccgc atacgcggcg aacacatcct ttccctcggc gaacgaacgc
    16021 tccagagccg gcatttcggt atcgagcacg cgccgcaact gctccccgcg ccacgcgatc
    16081 ttggccggaa agtacttgta cccctccgct tcggagtgga agccgatacg cgagtcggcc
    16141 cggcacaact ccttcagttc gacacctccg gccgcttcct tttcaacgat tccgcgcatc
    16201 tcgctgagca gggcgagctg ccgcgccttc acggtttccc gcgccagccg ctcacgcaga
    16261 tcatagaacc gcaagatgtt cagaccgctg cggaactgca accccagcgc gcgcgacacg
    16321 ccgatatcct gccgccgcgc cttcggcagg ccggaatcgc gctccagcgt ccgcaacagc
    16381 cccacgccct gatcccacat gacgctcatc gcgcgacacg cctcgatcgc ttcctgcaat
    16441 gtgtaagccc cgcccaaagc ctccgcgatg cggtctccgc tgggcgggaa gggatcccca
    16501 acccgctccc cccggttggc ggcgatcagc caggtcggtg ccaacggcgc gtcctgcggc
    16561 agcagcagca acggccagac caccccgtca tgcatgggtc cccagtactg aaacggtgtg
    16621 accagcggat agtgcccata cccctccgcg aaccgctccc aggcttgtgc caccgttccg
    16681 gctcgcgcgc ggccccattc ggcgcgcgcc agctccgata caaaagcctg ttccgtttcg
    16741 gggaacggtt cgaaagcgag ttcacccgcg gctttgttca tgatccccgg atagttgccg
    16801 aaataccagc agagcatcac ccggctcacc cccaactcgc gcatcgcccg gaatttgcgg
    16861 tacagcaatc ccggtaccgg cacaaacggc acggtcgcca cctcatgcga gcacccggtc
    16921 tggatcttgg ccgaaaaggc cacgcctgac tcccgtgcca aatccgccag ccgctcaaac
    16981 cgcgcactcg gtcccggcgt ggagatccag taatccccgc ccagccggac tcgtccgaaa
    17041 tccgttttcg tgaccccgga ctcgaagttg aactggtaga caacgttcga aagaccatgc
    17101 tgcgggatcg cgtacaccca cttccctgga tccgcgtcct gcggcatgta aaaccacgcg
    17161 atcagctcag catgcggatt ggcctcgcgc ataccccgtg agaggggttc cagcgtcgcc
    17221 tggaaaattt cccacggctc ttttttcgca cagacggggc aggtcgtgcg gccgttactg
    17281 gttgccacga ccacgctctg gcaactggtg taccgttcgc cgtgcgtgat gttgatcaga
    17341 ccgcccaagt gcgggaccgc cgaaaagatc cccttcgcgg cctcatacag ataacgttgc
    17401 gcggtctccg aactcgggca gaacatgagt ttgccgcctg ccttcgcgcc gcccagttcc
    17461 gggtggtttt tgagtaccgg gtcatccgca tcccacgccg caggctcaat cgcgaaaagg
    17521 tagatgcgaa tgccgtaccg acggcaccgt tcgaccgtgc gccgcagttt ggcgaaccgc
    17581 cgatcagcct gctcgccgaa tccgggcgtg atctccgtgc ggcacaggtc gcggaacgtc
    17641 acagtcagcc agaggccgtt gatgccctcg tgcgccagcc ggttgaggta cgcttcggga
    17701 taataatcca catcatccat caactcgtcg cggtatcgtg gcggacgttt gatcggcccg
    17761 aaaaagcaac ggg
//
LOCUS       DAOSRB010000040        17647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3337_length_17647_cov_6.940371, whole genome shotgun sequence.
ACCESSION   DAOSRB010000040 DAOSRB010000000
VERSION     DAOSRB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17647
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3337_length_17647_cov_6.940371"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..985)
                     /locus_tag="PLZ74_03685"
     CDS             complement(107..985)
                     /locus_tag="PLZ74_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOR97456.1"
                     /translation="MMKAGRILSVAFWVATGAVLADEPVCPLGSDWVLDATVSDEFNG
                     TGIDKAKWWDYAPRWRGRREFLYSHKNVEVRDGFLSLYARNVNPEEMAYEDREDGILP
                     YTCAIIKSRKKVTYGYFECRARGNAAEVRNAFWLYDPLSDRLSKKVRPGSHSEEIDIY
                     EFIGKYQDPSCLPYEICAHVHRFETPYVEGLVNHVKTKLANPGGAVAVDWAPCDGYHL
                     YGFLWTEKEMVWYVDGKEYFRRDNDYFHTPLHVMFDCEIATWRRASVKRLDPATLPAS
                     HSVDWFRRWVKRVPGR"
     gene            complement(1130..1450)
                     /locus_tag="PLZ74_03690"
     CDS             complement(1130..1450)
                     /locus_tag="PLZ74_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97457.1"
                     /translation="MRRFPLVPAMICVLLGIGDGMARVRVEETTVTLPTYPPGPYDKN
                     PIFYTGRVYQGAQGRVYPYPLQDTLHDEKVDQTYASLRLENECLEMTLLPNGSPTVRP
                     WRFI"
     gene            1763..2647
                     /locus_tag="PLZ74_03695"
     CDS             1763..2647
                     /locus_tag="PLZ74_03695"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOR97458.1"
                     /translation="MFFFDKIRRGEPTISFEFFPPKTPDGWARLYATIAETARLNVDF
                     VSVTYGAGGSTRESTVELVASLQNNLAIHAMAHLTCLGHSQAELARILDRLAEKRVPA
                     IMALRGDPPQGETAFRPHPYGFAHADDLIGFIRDGWPDFKIGCAAYPEGHPESFAPGE
                     ARTPDIDIGILRRKQENGADFAVTQLFFDNAVYFHFVDQARAAGVTIPLLPGIMPMSS
                     AKQIERFIALAGCSIPETLKQAARAPDVAEAGFLFALDQCRDLLTRGAPGIHLYSLNQ
                     AELSSRLLTALRDERLLR"
     gene            2689..4509
                     /gene="mutL"
                     /locus_tag="PLZ74_03700"
     CDS             2689..4509
                     /gene="mutL"
                     /locus_tag="PLZ74_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOR97459.1"
                     /translation="MVKDGHIRLLPIHIANKIAAGEVVERPASVVKELIENAIDAGAT
                     RLDITVTAGGRKLIAVSDNGCGMNRDDALLSIERQATSKITDVDDIERIDTLGFRGEA
                     LPSIASVSRFLLKTRRAEKDAGTELTVVGGSLQDVREAGLPPGTTIEVRDLFFNVPAR
                     RKFLRAYQTEQAHIRSVFTLHALAHPEIGFSLAADGRELARLPCDATHEERIRDLFGA
                     AFCAGLRPVDRSVRGVRVTGFAGLPNLTRTDRSEQYVFINRRPATAPVIAYALREAYP
                     PLEGNRKPIVLLFIELPPDEVDVNVHPTKREVRFRRPADVREAIILAVRAALGRKRET
                     RAAAPAGHQDEAAVTLRALVPEAAAQTRGEPFPYPGPVALPPTDAPAAAPPSPAKSLP
                     TEPPPAPTGSGLPLFHTPEDGSPWRWCRVMGQVGGRYLLLETDGGYVTLDPCAARERV
                     LYERLMTVHRSGGVVSQALLLPETVRLPPADAARLRKFLTVVQEMGFGIADFGSDHFM
                     VESLPDALAGVSCRELLGNIAQDLENVGTRRGADKWRQELVAKAACRAAAGGMAPLSD
                     DAAEKLVADLAETQMPYTCPRGRPTMIFTSYRELERKFGR"
     gene            4667..6355
                     /locus_tag="PLZ74_03705"
     CDS             4667..6355
                     /locus_tag="PLZ74_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97460.1"
                     /translation="MACRVLCAADADAQPAARVKAVVRILPQTVDVRNDAFLIGLTAG
                     CDGGAARAVRAERLPHEIPEAELRSSCPTGGAVRISVSHVPGETAPFYTVVNNSDTEQ
                     GVTLLAQALRQGVPHTLSVICRSERDKTRLTVGFQSAADPAAPETFRRARIPSGSKWN
                     RHTLTFTPDRDGTFRLRFLLEPGGIVTFARFSLMPADADENGWDPVALEALRVTAAPF
                     LFWPVCSGSGFYNWYDGVGPQAAREAKGQAFGTVEAVRLSRLSGAEPVFRISLFAPED
                     ASEATGIPRPAAAAELAADWVAYCNATGSHPLAMLRTRHGVTEPLGVRRWELCPAGGL
                     LRDPSAFARESRRVADAMRAEDATLTLQIRPALTNAYPVSDPYTGRLLKRLESADAAE
                     REYYRAWYEALGPLSARLDLRAAESDRDNTAPLAFTLPDVLCRNVSPPACLTGPGLLM
                     TLLNRFPLAGILATEGAPPTVRILAGWGEDDSVLLLFVCNTGTEAQTLHFDLRALRRR
                     FMFYTLDQAAAEITLPRLTPVLPIRRHQKAGAALTQFVVCEAAPSSFTRILVTE"
     gene            complement(6383..7249)
                     /gene="lsrF"
                     /locus_tag="PLZ74_03710"
     CDS             complement(6383..7249)
                     /gene="lsrF"
                     /locus_tag="PLZ74_03710"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HOR97461.1"
                     /translation="MADLDDIRDGKEYGVGTAQKTEGFFLKGSSHLDWGMKDRLSRIF
                     NPATGRTVMLAFDHGFIMGPTSGVERIDLSIVPLIEYADCLMCARGVLRSLVPPTCRK
                     PICLRSDAGTSILTELNHNVVIDVQDAVRLNASAIAVMVAVGDAATEAETVANLYQAV
                     DAGQACGIPVMGVTAVGKDMARDARYFGLASRLCAENGANIVKTYYTEGFEKVVACCP
                     VPIVIAGGKKLPELEALELAYRAIDSGAAGVDMGRNVFQSEKPAAMIQAVSAVVQQGL
                     KPKDAFQMFNDL"
     gene            complement(7277..7924)
                     /locus_tag="PLZ74_03715"
     CDS             complement(7277..7924)
                     /locus_tag="PLZ74_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOR97462.1"
                     /translation="MTTSGETNSMLTLARFKMMIAAAGDAIRAQEKRFSELDAAAGGD
                     GDHGTAIVTAFNAMSQADGADFRSYLKALSDALQNEACGSTSTLYGAWLQGMGEAAPE
                     GAAALDEAALAAVFQGGLEEIGFTTRARVGDKTLMDVLIPATGALVAAQGGGVPAMLA
                     KAAEAADRGAESTCALRAKFGRAKNLGDRSIGPCDAGAASMACIFAAFAKAAETK"
     gene            complement(7921..8913)
                     /locus_tag="PLZ74_03720"
     CDS             complement(7921..8913)
                     /locus_tag="PLZ74_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOR97463.1"
                     /translation="MKKFINDPANLTKELLEGLALCYKGKVKLVSERIVMRAVPKDES
                     KVAIVTLGGSGHEPALSGFVGEGMLDASVVGDVFAAPGAPKLLEALRLLKRDAGIVLV
                     TLNHDGDRMSATKALRDAEKEGIKVREIVTHDDIGPGVDADPNEKRGLAGCIPLYKVI
                     GAAAEEGKSLEEIVDIGERFNRHIATLAVAMTGCTHPQNGEPITVLPDDEMEIGMGQH
                     GEAGGGRTKILTADETALRMIEPLVKASGVKSGDTALLIINGVGATTLMEMLIVYRKA
                     AQILADKGINLTSGACGEYLTVQEMAGFQMILCKLDPDHVTYLKAKARTPYWTA"
     gene            complement(8950..9261)
                     /locus_tag="PLZ74_03725"
     CDS             complement(8950..9261)
                     /locus_tag="PLZ74_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97464.1"
                     /translation="MTYHHTGIPVFEKMEGMSFVEPLKVWVTDAGASPYKVEWLYFEP
                     DSPMAAAIQDETHVAYVVDNIEEAVKGKSVLWPVCEVAPGLKIAFIYDQGLPIELMQV
                     G"
     gene            complement(9286..9885)
                     /locus_tag="PLZ74_03730"
     CDS             complement(9286..9885)
                     /locus_tag="PLZ74_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97465.1"
                     /translation="MNNADYYKDGKFDSEKGKQAYFDLMKKFNVPVYAELARPDGPFW
                     AVDFAKGDFAAFGMGGVIWCNELAEGYFGHDIYLLPCQSIAEHRHLPTTDKDGKPIRC
                     KIESWQVRHGYVYGFSEVGEPNLDKFPEAKAMLSKVQIPHLKSVHVEKWEADGKVHKL
                     AKPETWHFMMGGKDGAIVTEYATYHDGAGLRFSVPGVGF"
     gene            10512..11609
                     /locus_tag="PLZ74_03735"
     CDS             10512..11609
                     /locus_tag="PLZ74_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOR97466.1"
                     /translation="MRILSRYVLREFLVPLFYCLAGFLGIYVLFELFDSFNRLMEARP
                     PFLEVVAFFAGYVSPYLEWLFPAALLLGTLYTMWNFCRHSEITAMRANGIGFTAIVRP
                     LLVVAALMALAVAAINEFYAPHAAEQARAMRENRFKPLTNTEILNIPYYNQQARRVWR
                     INHMRSDQPNRIEGVRISFDRPDGSRQLDITCRSAEYLDGLWWLFYPQYQYFDELNNP
                     IENPKPQLADLVIRAMPELNESPRDFLLMNKAWEFYSVRDMLHYLKNHPHLEKHEVAS
                     KTFDVHARLAAPFSCLIITLFAIPAGMASGRQSVFKGVLLAVALFFGFYVVTNGCMVL
                     AKNGLMPVILGAWCANVIFLAAGLALFYRQR"
     gene            11624..12328
                     /gene="trmB"
                     /locus_tag="PLZ74_03740"
     CDS             11624..12328
                     /gene="trmB"
                     /locus_tag="PLZ74_03740"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOR97467.1"
                     /translation="MPMNAVPDTPTPAPAALRTHVIPISDPTRPFTPDILSNDPARPL
                     EVEIGCGNGRFLATRASRNPDTAYLGIERLLGRVRKLDKKAIRLNLGNLRILRLEACY
                     TFYYLLPRHSVRTVYVFFPDPWPKRRHHERRLFSPLFLDALWARLEYGGAIQIATDHR
                     DYFAEIRTRFAADPRFAEIPAMTRTDEEQTEFEQLFRNQGLPIYACAYRALPAEEPAL
                     PPLTLAPDMEPQAERE"
     gene            12583..15822
                     /gene="mfd"
                     /locus_tag="PLZ74_03745"
     CDS             12583..15822
                     /gene="mfd"
                     /locus_tag="PLZ74_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOR97468.1"
                     /translation="MSHRFTELLTPRLPDQPACRAVSALPGASAALTAVLLARTAPAR
                     FALTVTPGAVELERVYGDLCALGQDRGVAPLLFPPLQEDDPEAAGSRMRVIRILQTEC
                     KPHAERDAAPTVIVTSLAALLHPVPDPQALAAASVHLRVGESFPFESLVAQRVESGYT
                     RVTEVEAPGQLAVRGGLLDIWPPAAPAPWRAEFFGDELEALRTFDPATQCSVAPGQEV
                     WLPPCSETGLATLHLETLLPPHASVLWLDHDLIRAGADAPPPSTTGHARPASRAPAFW
                     DTLAARINTREPWLQLFAGDPPPARTPTLDFAIAALPGLAEWSAENAHHPELLTGVRQ
                     RLLAELARAADAGQTVLLYADTPGTCELLSRELGPDTRVHVCRAPLSGGFTLPGLTVA
                     AQPDLYAAYKPVRSRRPPAAAVRGGRVENAADLEPGDLVVHVDHGIGRFLGSTEVEID
                     DSRSEVFTIEYAEGAKLYVPVSHAHLLSRYVGVAGHKTTLHRLGGRRWTREKADAERA
                     VADLAASLLDTQARRQVAEGLVFNPEPQWMREFEAAFPYQETPDQERVIAEVKRDMAA
                     PRPMDRLICGDAGYGKTEIAMRAAFIAVMNGRQVAVLVPTTVLAEQHYETFSDRMAAY
                     PVKIEVVSRFHSYAKRRRILTETRHGRVDILIGTHALLSEHVSFQNLGLLIIDEEQRF
                     GVSHKERLKQIRQIVDVLTLSATPIPRTLYLSMTGARDMSLLQTPPHERMAVETKVAR
                     DSDTVIRNAIRLELNRRGQVYLLYNRVLTIELMRQRLEKLVPDARIAVAHGQMPSAEL
                     AHIMRAFEAGGIDVLLCTTLVESGLDIPRANTILIHRADRFGIADLYQLRGRVGRSSQ
                     KGFAWLLLPEHGHVDEDARQRIAAIQKYSGFGAGFNLALRDLEIRGAGNLLGTAQSGH
                     IAAIGFNLYCQLLRRTIARLKGEALPLLVDVELALDFLDLSPGTAHDETSACLPYAYV
                     EDEAHRMILYRRLAEAVSVKEVRALRDELTDRYGRPPAAVTRLLRLTELRILAAQKRI
                     GRIETHNGKIHLFRSHERSPLLIRNRLPRLKGTRTDTRLDALFQTVRSL"
     gene            complement(17389..17647)
                     /locus_tag="PLZ74_03750"
                     /pseudo
     CDS             complement(17389..17647)
                     /locus_tag="PLZ74_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653701.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
BASE COUNT         3132 a         6240 c         5048 g         3227 t
ORIGIN      
        1 acacccgatc catcgtctca tccggaatcc cccccccctc gcctttcaca tcacatgtcc
       61 gtttccttat cgccacccgc gcccgcgctc ctgccaccca tcctgatcac cttccgggca
      121 cccgcttcac ccagcgccgg aaccagtcca cgctgtggga cgcaggcagg gtggcaggat
      181 ccaaccgctt aaccgaagcc cgccgccatg tggcgatctc gcagtcgaac atcacatgca
      241 gcggcgtgtg gaaataatcg ttgtcgcggc ggaagtactc tttgccgtcc acataccaga
      301 ccatctcctt ctccgtccag agaaagccgt agaggtggta cccgtcacac ggtgcccagt
      361 ccaccgccac cgcgccgccc ggattcgcca atttggtctt cacatgattg acaagccctt
      421 caacatacgg cgtctcgaac cggtgcacat gcgcgcagat ctcgtagggc aaacaggagg
      481 gatcctgata cttgccgata aactcgtaaa tgtcgatctc ctcggagtga ctgcccggac
      541 gaaccttttt ggagagccgg tcgctcaaag gatcgtagag ccagaaggcg ttgcgcacct
      601 cggccgcgtt tccgcgcgcc cggcactcga aatagccgta cgtcaccttc ttacggctct
      661 tgatgatcgc gcaggtgtac gggagaatcc cgtcctcgcg atcctcgtac gccatctcct
      721 cggggttcac gttacgcgcg tagagcgaca gaaaaccgtc gcgcacctcg acatttttgt
      781 ggctgtagag aaactcccga cgcccccgcc aacgcggcgc ataatcccac cacttcgcct
      841 tgtcgatccc cgtgccgttg aattcgtcgc tgacggtcgc atccagcacc cagtccgaac
      901 ccaacggaca gaccggctcg tccgccagaa ccgccccggt tgccacccaa aacgccacgg
      961 atagaatccg tcctgctttc atcatccacc tctccttttt ttctgtccca tcccctttat
     1021 cggctcagga aataccgggc ccaaagcaga tccgggcgtt ccttcaaagc ctccccgaaa
     1081 agtttcttgg cctcctccga acgattctcc gccagagcca gtagcccttt cagataaagc
     1141 gccacggccg tacggtgggt gacccgttcg gaagcagcgt catctcgaga cactcgtttt
     1201 ccagccggag cgaggcgtac gtctgatcca ccttctcatc gtgcagcgtg tcctgcaacg
     1261 gataagggta gacgcgaccc tgcgcaccct gataaacgcg gccggtatag aagatcggat
     1321 tcttgtcgta cggccccggc ggataggtcg gcaacgtcac cgtagtctcc tccacccgaa
     1381 cccgcgccat tccgtccccg atacccagca gcacgcaaat catcgccgga accaacggaa
     1441 aacgtctcat cccaataccc tcttcttggt tgtgaagcca gcatacaccc tctgatcaaa
     1501 cacaacaagt cacgtttgca cagaaaaccc cgtgcaggaa cggaaagcat cgtccccgcc
     1561 ctcgttctcg tgcacatcgg cccgcgaccc gcgacattcg gcctgcgacg ttgcggcacg
     1621 ggcatcctgc ccgtgtttca tgggcgggac cccatgttgc cactgatcca ctcctcgccc
     1681 cgcgttgccg accggaacca gccgaaaccg cttggcaagc agtgggcgat ggccgtatac
     1741 taaagggtct tgagaggttc tgatgttttt tttcgacaaa atacgccgtg gtgaaccgac
     1801 gatctcgttc gagtttttcc ccccaaaaac cccggacggc tgggcccggc tttacgctac
     1861 gatcgccgaa accgcccggc tgaatgtgga tttcgtttcg gtgacctacg gcgcgggcgg
     1921 cagcacgcgc gaaagcacgg tggagctggt cgcctcgctg cagaacaacc tcgcgatcca
     1981 tgccatggcc cacctcacct gcctcgggca ctcgcaggct gagttggccc ggattctgga
     2041 tcgcttggca gagaagcgtg tgcccgccat catggcgctg cgcggcgacc cgccgcaggg
     2101 cgagaccgct ttccggccgc acccttacgg tttcgcccat gcggatgacc tgatcggttt
     2161 tatccgcgac ggctggccgg acttcaagat cgggtgcgcc gcctatcccg aagggcaccc
     2221 cgagtccttc gcccccggcg aggcgcgtac cccggacatc gacatcggca tcctccgacg
     2281 gaaacaggag aacggcgccg atttcgcggt cacgcaactt ttcttcgata atgccgtcta
     2341 cttccacttc gtcgaccaag cgcgtgccgc cggggtgacg atccccctgt tgccggggat
     2401 catgccgatg tcatccgcaa agcagatcga gcgtttcatt gccctagccg ggtgcagcat
     2461 cccggaaacg ctgaaacagg cggcccgggc accggatgtg gccgaagcgg gttttctttt
     2521 cgcgttggat cagtgccgcg acctgttgac gcgcggcgcg ccggggatcc atctctattc
     2581 actcaatcag gcggaactct ccagccgcct gctcaccgcg ctccgcgatg agcgcctcct
     2641 tcggtaaagt ggcttgacgc tccgccgaca acccgtcatg ctctcgtcat ggtcaaagac
     2701 ggacacatcc gcctgctccc cattcacatc gccaacaaga tcgcggcggg tgaggtggtc
     2761 gagcggcccg cttcggtagt caaggaactg atcgaaaacg ccatcgatgc cggggccacg
     2821 cgtctggaca tcacggtcac ggccggcgga cgcaagctga tcgcggtctc tgacaatggc
     2881 tgcggcatga accgcgacga cgcgttgctc agtatcgagc ggcaggccac cagcaaaatc
     2941 accgatgtgg atgacatcga acgcatcgac acgctcggct tccgcggtga ggcgctgccg
     3001 tcgatcgcct cggtgtcgcg tttcctgctc aagacccgcc gcgccgaaaa ggacgcgggc
     3061 accgaactca ccgtggtcgg aggcagtctc caggacgtgc gcgaagccgg cctgccgccc
     3121 ggaaccacga tcgaagtccg ggacctgttc ttcaatgtcc cggcccgccg caagttcctg
     3181 cgcgcatatc aaaccgaaca ggcgcacatc cggtcggttt tcaccctgca cgcgctggcc
     3241 catcccgaaa tcggcttcag cctcgccgca gacggtcgcg aactggcgcg cctgccgtgc
     3301 gacgccacac acgaggagcg gatccgcgat cttttcggcg cggcgttctg tgccggactg
     3361 cgcccggtgg accggagcgt gcgcggcgtg cgcgtcaccg gattcgcggg actgccgaac
     3421 ctcacccgca cggaccgcag cgaacagtac gtcttcatca accgccgccc cgcgaccgcg
     3481 ccggtcatcg cgtacgcatt gcgcgaggcc tacccgcctc tggaaggcaa ccgcaaaccg
     3541 atcgtgctgt tgttcatcga gcttccgccg gacgaggtgg acgtgaacgt ccatcccacc
     3601 aaacgcgaag tccggttccg cagacccgcc gacgtgcgcg aggccatcat cctcgctgtc
     3661 cgtgccgcgc tcgggcggaa acgcgagacg cgcgccgccg ctccagcggg acatcaggac
     3721 gaggccgccg tgaccctgcg cgcgctggtg ccggaggctg ccgcgcagac acgaggcgaa
     3781 cccttcccct accccggacc ggtggcgctg ccgcccacgg acgcaccggc ggcggcaccc
     3841 ccttccccgg ccaagtccct gccgaccgaa ccgccgcccg ccccgaccgg ttcgggactg
     3901 ccgctattcc acacgcccga agacggctcg ccgtggcgtt ggtgccgtgt gatgggacaa
     3961 gtcggcggac ggtatctgtt gctggagacc gatggcggat acgtgacgct ggacccctgt
     4021 gccgcgcgcg agcgggtgct gtatgaacgg ctgatgaccg tacatcgatc cggcggcgtg
     4081 gtctcgcaag cgttgctgtt gcccgaaacc gtgcgcctgc cgcccgccga tgccgcgcga
     4141 ctacgcaagt tcctcaccgt cgtgcaggaa atgggtttcg gcatcgcgga tttcggcagc
     4201 gaccatttta tggttgagag cctgccggat gcgctggccg gtgtctcctg ccgtgaactg
     4261 ctcggcaaca tcgcgcagga tttggaaaat gtcggaacgc gccggggcgc ggacaagtgg
     4321 cgccaagaac tggtggccaa ggccgcatgc cgggccgccg caggtggcat ggcgcccctc
     4381 tccgacgatg cggccgagaa gctggtggcg gatctggccg aaacgcagat gccctacacc
     4441 tgtcctcgcg ggcgccccac catgatcttc acctcgtatc gtgagttgga acgcaaattc
     4501 ggacggtaat acggaggtgt ttgtgcctta cgaaccaaca agcacaatac aaaacaagtc
     4561 gtttctcaca cgaaggcacg aaaaagagtg gtccccgagc cgggagcgcg ggcgcccccg
     4621 cccgcttcgg aggagagcgc cacgttttgt tgttgcgtcg ttcgcgttgg cctgccgcgt
     4681 cctgtgtgcc gcagacgcgg atgcgcaacc cgcggcgcgt gtcaaggcgg tggtgcggat
     4741 tctgccccaa acggttgacg tccgtaacga cgcgttcctg atcgggctga ccgccggatg
     4801 cgacggcggg gccgcgcgag ccgtgcgggc cgaacgtttg ccccatgaaa taccggaagc
     4861 cgaactgcgg agttcatgcc cgactggcgg agcggtcaga atctcggttt cacacgtccc
     4921 gggcgagacc gccccctttt acacggtggt gaacaacagc gacacggagc agggcgtgac
     4981 gctgcttgcc caagccctgc gccaaggtgt gccccacacc ctgtccgtaa tctgccgcag
     5041 cgagcgggat aagacccgcc tgaccgtggg attccaatct gcggctgacc cggccgcgcc
     5101 ggagacgttc cggcgggcgc gtatcccgtc gggcagcaaa tggaaccgcc acacgctgac
     5161 cttcacgccc gaccgcgacg gcaccttccg tttgcgtttt ctgctggagc cgggcgggat
     5221 cgtcaccttc gcccgctttt cgctgatgcc ggccgatgcc gacgagaacg gctgggaccc
     5281 tgtggcgctg gaagcgttac gcgtgaccgc cgcgccattc ctgttctggc cggtatgctc
     5341 tgggagcggg ttctacaact ggtacgacgg cgtgggcccg caggcagcgc gcgaagcgaa
     5401 aggccaagcc ttcggcacgg tcgaggcggt ccgtctcagc cgcctctccg gtgcagagcc
     5461 ggttttccga atttcccttt tcgctccgga agacgcctcg gaagcaacgg ggatcccccg
     5521 tcccgcggcc gcagcggaac tcgccgcaga ttgggtggcc tactgcaacg cgaccggcag
     5581 ccatccgctc gcgatgctgc ggacccgaca cggcgtgacc gaacccctgg gtgtacggcg
     5641 gtgggaactc tgccccgcag ggggtttgct gcgggatcct tccgctttcg cccgcgaaag
     5701 ccgccgcgtg gcagacgcca tgcgcgcgga ggatgcaacc ctcaccctgc aaatcaggcc
     5761 ggctttgaca aatgcgtatc ccgtgtcaga cccgtatacc ggccgtttgc tgaaacgcct
     5821 tgaatcagcc gatgcggcgg aacgcgaata ctaccgcgca tggtacgaag cgctcgggcc
     5881 gctcagcgcg cggctggacc ttcgtgccgc ggaatccgat cgcgacaaca ccgctccact
     5941 cgctttcacg ctgccggacg tgctgtgccg caacgtatcc cctcccgcct gcctgacggg
     6001 accgggcctg ctgatgaccc tgctcaaccg cttccccctc gcgggaatcc tcgcgacgga
     6061 gggcgccccg ccgaccgtgc gcattctggc gggatggggc gaggacgact cggtcctgct
     6121 tttgttcgtc tgcaacaccg gcaccgaagc gcagacccta catttcgatc tgcgggccct
     6181 gcgacgccgc ttcatgtttt acacgctcga tcaggccgcc gcggaaatca ccttgccgcg
     6241 tctgactccc gtgctgccga tccgccgaca tcagaaagcc ggtgccgcgc tgacacagtt
     6301 cgtggtctgc gaagccgcgc cctcctcctt cacgcgcatt ctggtcaccg agtgagaaac
     6361 ggaaaccgtc ttgcgcgtcg gctcacaaat cgttgaacat ctggaaggcg tccttcggct
     6421 tcagcccctg ctgaaccacc gccgaaacgg cttgaatcat cgccgcaggt ttttcggact
     6481 ggaacacgtt acgtcccatg tccacccctg ccgcaccgct atcgatcgcc cggtacgcca
     6541 gttccagagc ctcgagttcg ggcagcttct tgccgccagc gatgacgatc ggaacgggac
     6601 agcacgccac gaccttctca aacccctcgg tgtagtaggt tttgacgatg ttcgcgccgt
     6661 tctccgcgca cagccgcgag gcgagcccga agtaccgcgc gtcgcgtgcc atatccttgc
     6721 cgaccgccgt cacccccatc acggggatcc cgcaagcctg acccgcatcc acagcctgat
     6781 acaggttggc cacggtctcc gcctcggtgg ccgcgtcacc cactgcaacc atcaccgcga
     6841 ttgccgaagc gttgagacgc accgcgtcct gaacatcgat caccacgttg tggttgagtt
     6901 ccgtcaggat cgaggtgccc gcgtcggaac gcaggcagat cggcttgcga caggtaggcg
     6961 gcacaagcga acgcaacacg ccgcgggcgc acatcagaca gtcggcatac tcgatcagcg
     7021 gcacgatgct caggtcgata cgctcgacgc cggaggtcgg gcccatgatg aagccgtggt
     7081 cgaacgccag catcacggtc cggccggtag cggggttgaa gatccggcta aggcggtcct
     7141 tcatgcccca gtcgaggtgg ctcgatccct tgagaaagaa gccctcggtt ttctgcgccg
     7201 taccgacgcc atactcttta ccatcacgga tatcatccaa atcagccatt tcatactctc
     7261 ccgccgggtc tcccggtcac ttcgtttccg ccgccttcgc gaacgccgcg aaaatgcagg
     7321 ccatggacgc cgcgcccgcg tcgcagggac cgatcgagcg atccccgaga ttcttggcgc
     7381 gtccgaattt cgcgcgcaac gcgcaggtgg actccgctcc ccggtccgca gcttcggcgg
     7441 ccttcgccag catggccggc acgcctccgc cttgcgcggc aaccaaagcc ccggtggcag
     7501 ggatcaacac gtccatcaac gtcttgtcgc caacgcgtgc gcgcgtcgta aagccgatct
     7561 cctccagccc cccctggaac accgcggcca acgccgcctc atccagtgcg gcggctcctt
     7621 cgggcgcggc ctcgcccata ccctgaagcc acgcgccgta gagtgtgctg gtcgaaccgc
     7681 acgcctcgtt ttgcagcgcg tcgctcagtg ccttgaggta gcttcggaaa tccgccccgt
     7741 cggcttggga catggcgttg aacgccgtga cgatcgcggt gccgtgatcg ccgtccccgc
     7801 cagcggcggc atccaactcg gagaaacgct tctcctgcgc acggatggcg tcgccagcgg
     7861 cggcgatcat cattttgaac ctcgctaacg tcagcatgga gttcgtctct cctgatgtcg
     7921 tcatgccgtc cagtagggcg tacgggcctt ggccttcaga tacgtcacgt ggtcgggatc
     7981 gagtttgcac aggatcatct ggaagccagc catctcctgc acggtcagat actcgccgca
     8041 cgcgccggac gtgaggttga tccccttatc ggcaagaatc tgcgcggcct tgcggtagac
     8101 gatcagcatc tccatcaggg tggtcgcgcc gacgccgttg atgatcagca gcgcggtgtc
     8161 accggacttg acgccgctcg ccttcacaag cggctcaatc atccgcaagg cggtctcgtc
     8221 cgccgtgagg atcttggtgc ggccaccgcc ggcctcgccg tgctggccca tgccgatctc
     8281 catctcgtca tccggcagca ccgtgatggg ctcgccgttc tgcggatgcg tgcagccggt
     8341 catagccacc gccagcgtcg cgatatgacg attgaagcgc tcgccgatgt ccacgatctc
     8401 ttcaaggctc ttgccttcct cagccgcggc accgatgacc ttatagagcg ggatgcagcc
     8461 ggccaagccg cgtttctcat tcgggtcagc atccacaccg ggtccgatgt cgtcgtgcgt
     8521 cacgatctcg cggaccttaa tgccctcctt ctccgcgtcg cgcaacgctt tggtggcgct
     8581 catgcggtcg ccgtcatgat ttagcgtgac cagcacgatg cctgcatcgc gcttgagcag
     8641 gcgcagcgct tcgagcagct tgggcgcgcc cggcgcggca aaaacgtcgc ccaccacact
     8701 cgcgtccagc atgccctcgc ccacaaaccc gctcagggcc ggctcatgtc cggagccgcc
     8761 cagcgtgacg atcgcaacct tgctctcgtc cttgggcacg gcgcgcatca caattctctc
     8821 actcacgagc ttgaccttgc ccttgtagca caaggccaac ccttccagca gttccttggt
     8881 aaggtttgcc ggatcattaa tgaatttttt catcgccatc gcaatttctc ccggttacac
     8941 gttttacaat cagcccacct gcatcagttc gatcggcaga ccctggtcgt agatgaaagc
     9001 gattttcagc cccggcgcca cctcgcacac cggccacagg acgcttttgc ccttaaccgc
     9061 ctcttcgatg ttgtcgacca cgtacgcgac gtgggtctca tcctgaatgg ccgcagccat
     9121 gggactgtcc ggctcgaaat agagccactc caccttgtac ggactcgcgc cagcatcggt
     9181 gacccacacc ttcagcggct ccacgaacga catcccctcc atcttttcga aaacggggat
     9241 tccagtgtga tgatacgtca tgttttgcct ccttgttgaa ctgatttaga aacccacccc
     9301 cggcaccgag aaacgcaggc ccgctccgtc gtgatacgtc gcgtattcgg tcacaatcgc
     9361 cccgtccttg ccgcccatca taaagtgcca cgtctcgggt ttggccagtt tgtgcacttt
     9421 gccgtccgcc tcccatttct cgacatgcac gcttttcaga tgcgggatct gaactttgga
     9481 cagcatcgcc ttggcttccg ggaacttgtc caaattgggc tcgccaacct cgctgaagcc
     9541 gtagacataa ccatggcgca cctgccagct ctcgatcttg cagcggatcg gcttgccgtc
     9601 cttgtccgtc gtgggcaggt ggcgatgttc ggcaatgctc tgacacggca gcaggtagat
     9661 atcatgtccg aaataccctt ccgccagctc attgcaccaa atcacgccgc ccataccgaa
     9721 cgcggcgaaa tctcccttgg cgaaatccac ggcccagaag ggtccgtctg gccgcgccag
     9781 ttcagcgtac accggcacat tgaacttctt catcagatcg aaataggcct gcttgccctt
     9841 ctcgctatcg aatttcccgt ccttgtaata atccgcatta ttcactcgct tacatccttt
     9901 ctgcgccgca cctccgtggc actttgcaac tcctcccgac gtctgacagc ccgctgaaaa
     9961 acccaacagg cccatcccca tcccggcgac cattacgccg gtcaaaacat tcgacaaaac
    10021 agctttctcc attgtaatcc tcctttgcgg gtcatcaccg gctccatacc ggattccaga
    10081 ccccgcatac gcacgaatcg tagttaaggc agtaagttta ccgttccatc catcgcctgt
    10141 aaaggcgaaa gaggaatacc cttcggttcc gggtttctgg tttatcgaaa tcgctatcgg
    10201 gatcgcttcc ccgtccccat tctcgtcccc gttttcgtgt acagcgactt gcgacccgcg
    10261 acatccgacc tgcgacaggg aggcctccgg tccgccctgt ccaccctgtc agacgaaaaa
    10321 caagccgttg cgggcaggga cgtccggctc cagactcccg cgcgaatgca ccccccgccc
    10381 cgcgttcccg actgaaaccc ccaacccgga acctgaaacc caagaaccgt tgagcgggca
    10441 gatttcggtt ccggtccgta ataaaacgga ttgccgaacc ggcaaacgct accgtatact
    10501 gaaccacgtc tatgcgaatc ctgtcccgat atgtcctgcg cgagttcctg gtgccgcttt
    10561 tctactgcct ggccgggttc ctcggcatct acgtcctgtt cgaactcttc gactccttca
    10621 atcggctgat ggaggcccgc ccgccttttc ttgaggtcgt cgcgttcttc gcgggctacg
    10681 tctcgcccta cctcgaatgg ctcttccccg ccgcgctgct cctcggcaca ctctacacga
    10741 tgtggaactt ctgccgccac agcgagatca ccgccatgcg cgccaacggg atcgggttta
    10801 cggccatcgt gcggcccctg ctcgtggtcg cggccctcat ggcgctcgcc gtcgccgcaa
    10861 tcaacgaatt ctacgcgccg cacgccgcag aacaggcgcg cgccatgcgg gagaaccggt
    10921 tcaagcccct gaccaacacc gagatcctga acattcccta ctacaaccaa caggcccgcc
    10981 gcgtctggcg catcaaccat atgcggtccg accagccgaa ccggatcgag ggggtccgca
    11041 tctcgttcga ccggccggac ggcagtcggc aactcgacat cacctgccgc tccgccgagt
    11101 atcttgacgg actctggtgg ctgttctatc cccagtacca gtattttgac gagctgaaca
    11161 acccgatcga aaaccccaag ccccaactgg ctgatctggt gatccgtgcc atgccggaac
    11221 tcaacgagtc gccccgcgac ttcctgttga tgaacaaggc ctgggagttc tactccgtgc
    11281 gcgacatgct gcactacctc aaaaaccacc cgcatctcga aaagcatgag gtcgcctcaa
    11341 aaaccttcga tgtccacgcc cgcctcgcgg cgccgttctc gtgcctcatc atcacgctgt
    11401 tcgcgatccc cgcaggcatg gcctcgggac gccagagcgt cttcaaaggc gtgctcctcg
    11461 cggtcgccct cttcttcggt ttctatgtcg tgaccaacgg ttgcatggtg cttgccaaaa
    11521 acgggctcat gcccgtgatc ctgggtgcct ggtgcgccaa cgtgatattc ctcgccgcag
    11581 gcctggccct cttctaccgc cagcgctgag aaaccgaaac cgcatgccga tgaacgccgt
    11641 ccccgacacg ccgacacccg ccccagccgc actgcgcaca catgtgatcc cgatatccga
    11701 ccccacccgg ccgtttacgc cggacatcct gtccaatgac cccgcgcgtc cgctggaagt
    11761 cgagatcggc tgtggcaacg gccgttttct tgcgacccgt gcgtcacgca accccgatac
    11821 cgcctacctt gggatcgaac gcttgctggg ccgcgtccgg aaactcgaca agaaggcgat
    11881 ccgccttaac ctcgggaacc tccgcatcct gcggctcgag gcctgctaca ccttctacta
    11941 tctgttaccg cggcacagcg tccgcaccgt ctatgtcttc ttccccgatc cctggcccaa
    12001 acgccgccac cacgagcgtc gcctcttttc cccgttgttc ctcgacgcgc tctgggcacg
    12061 tctggaatat ggcggcgcca tccagatcgc cacggaccat cgcgattatt tcgctgagat
    12121 ccgcacgcgc ttcgcagccg acccccgttt cgccgaaatt ccggccatga cgcgcactga
    12181 cgaggaacaa accgaattcg agcagctctt ccgcaatcaa ggcctgccga tctacgcgtg
    12241 tgcctaccgt gcgctgcccg ccgaagaacc cgccctcccg cctctcacac tcgccccgga
    12301 tatggagccg caagccgaac gggagtgagg gttggtctct ggttccaggg cggggggttc
    12361 agtcggaagc gcgggacggg gagtgcgttt tttagtcgga cgagtcgaat cgcaggtctc
    12421 cctgtcgcag gtcgaatgtc gcgggtcgca agtcgacgca cttgcccttt ccgttcctgg
    12481 tttcttgtgt ttcatggtac gaacgccgcg tttccctcat gcgttcgttg cactctcgcc
    12541 gcaccccgcg tttgtgctat actcacggct actgtgatct cgttgtcaca ccgttttacc
    12601 gaattgctga caccgcgcct gcccgaccaa ccggcgtgcc gggccgtgtc cgccctgccc
    12661 ggcgcaagcg ccgcgctcac cgccgttctc ctcgcgcgca ccgcgcccgc gcggttcgcc
    12721 ctgaccgtga ccccgggagc ggttgaactc gagcgcgtgt atggcgacct ctgtgccctc
    12781 ggccaagaca ggggtgtcgc tcccctactc ttccccccct tgcaggagga cgaccccgag
    12841 gccgcgggca gccggatgcg ggtgatccgg attctccaaa cagaatgtaa accgcacgcg
    12901 gagcgcgatg cggcgccgac cgtgatcgtg acctcgctgg ccgcgctcct gcaccctgtg
    12961 ccggatccgc aagccctcgc tgcggcctct gttcacctgc gcgtcgggga gtccttcccc
    13021 ttcgagagcc tggtcgcgca acgagtggag agcggatata cgcgcgttac ggaagtggag
    13081 gcgccgggcc aactcgccgt gcgcggcgga ctgttggaca tctggccacc cgccgcaccc
    13141 gcaccgtggc gcgcggagtt cttcggcgat gaactggaag ccctccgcac gttcgacccc
    13201 gcgacgcagt gttcggtcgc cccgggacaa gaggtctggc tgccgccctg ttccgaaacc
    13261 ggtctggcga cgctacacct cgaaacgctg cttccgcccc acgcctccgt gttgtggctg
    13321 gatcacgatc ttatccgtgc gggcgcggac gcaccgcccc ccagcaccac cggacatgcc
    13381 cgccccgcga gccgcgcacc cgctttctgg gatacgctcg ccgcgcggat caacacgcgt
    13441 gaaccgtggt tgcaactctt tgcgggcgat ccgccgcccg cgcgcacacc aacgctggac
    13501 ttcgcaatcg ccgcgttacc cggactcgcc gaatggagcg cggaaaacgc ccatcacccg
    13561 gagttgctca cgggtgtgcg gcagcggctg ctcgccgaac tcgcgcgtgc cgcggatgcg
    13621 ggacagaccg tgctcctcta tgctgacacg cccggcacct gcgaattgct gtctcgggaa
    13681 ctcggccccg acacacgcgt ccacgtgtgc cgcgctcccc tctctggcgg attcaccctt
    13741 cccggcctca ccgtcgccgc gcaacccgac ctgtatgccg cgtacaaacc cgtccgcagc
    13801 cgtcgcccgc ccgcagccgc cgtacgcggc ggccgtgtgg aaaacgcggc ggatctcgaa
    13861 cccggcgatc tggtggtgca tgtcgatcac ggcatcggac gttttctcgg ctccaccgag
    13921 gtggagatcg acgactcccg ctccgaggtg ttcacgatcg aatacgcgga gggcgccaaa
    13981 ctgtacgtgc cggtctcgca tgcgcacctg ctcagccgct acgtgggcgt ggcaggccac
    14041 aaaaccacgc tccaccggtt gggcggacgc cgctggacgc gcgaaaaggc cgatgccgaa
    14101 cgcgcggtcg cggatctggc cgcgtccctg ctggacaccc aagcacggcg gcaggtcgcc
    14161 gaaggcctcg tctttaatcc tgaaccgcag tggatgcgcg aattcgaagc cgccttcccg
    14221 tatcaggaga cccccgacca ggagcgcgtg atcgccgagg tgaagcgcga tatggccgca
    14281 ccgcgcccaa tggaccgcct catctgcggc gatgccggtt acggcaaaac cgaaatcgca
    14341 atgcgggccg ccttcatcgc ggtgatgaac ggccgtcagg tggccgtgct ggtccccacc
    14401 accgtcctcg ccgaacagca ctacgaaacc ttttccgacc gcatggccgc ctatcccgtg
    14461 aagatcgagg tggtaagccg cttccattcg tacgccaaac ggcggcgcat ccttaccgag
    14521 acccgccacg gccgcgtaga cattctgatc ggcacgcacg ccctgctgtc ggaacacgtt
    14581 tctttccaga acctcggcct gctcatcatc gacgaggaac aacgcttcgg cgtgtcccat
    14641 aaggaacggc tgaagcaaat ccggcagatc gtggatgtgc tcacgctctc ggccacgccg
    14701 atcccgcgca cgctctacct gagcatgacc ggcgcgcgcg atatgagtct gctgcagacc
    14761 ccgccgcatg aacgcatggc cgtggaaacc aaggtggcgc gcgattccga caccgtgatc
    14821 cgcaatgcga tccgtctcga gctgaaccgc cggggccagg tctatctcct ttacaaccgc
    14881 gtgctcacca tcgagctgat gcggcaacgg ctggagaagc tggttcccga tgcgcggatt
    14941 gccgtggcgc acggccagat gccgtccgct gaactggccc acatcatgcg cgccttcgag
    15001 gcggggggga tcgacgtgct gctgtgcacc acgctggtgg agagcggcct cgacatcccg
    15061 cgcgccaaca cgatcctgat acaccgcgcc gaccgtttcg gtatcgccga cctctaccag
    15121 ctccgcggcc gggtcggccg ctcttcgcaa aaggggttcg cctggctgct gctgcccgaa
    15181 cacggtcacg tggatgagga cgcgcgccag cgcatcgccg cgattcagaa gtacagcggg
    15241 ttcggcgcgg gcttcaacct cgcgctgcgc gacctcgaga tccgcggcgc gggaaatctg
    15301 ctgggcaccg cacagagcgg ccatatcgcg gccatcggct ttaacctcta ctgccagtta
    15361 ctgcgacgca cgatcgcgcg cctgaagggc gaagccctgc ccctattggt ggatgtggaa
    15421 ctcgcgctcg acttcctcga cctctcgccc ggcacagcgc acgacgagac ctcggcctgt
    15481 ctgccgtacg cgtatgtgga ggatgaggcg caccgcatga tcctttaccg acgcctcgca
    15541 gaggcggtca gcgtgaaaga ggtccgcgcg ttacgcgacg aactgaccga ccgctacggc
    15601 aggccgcccg ccgcagtcac ccgcctgctg cggctgacgg aactgcgcat tctggcggcg
    15661 caaaaacgga tcggccgcat cgagacgcat aatggcaaaa tccacctttt ccgctcgcac
    15721 gagcgctcgc cgttgctgat ccggaaccga ctcccccgcc tcaagggaac ccggaccgac
    15781 acgcgtctcg acgccctgtt ccaaaccgtg cgctcgctct gacccgcggg acgacactct
    15841 ccccgcctgt cactttccct tttccgcctc cccgtcccgt accacctcgc gcacgttgtt
    15901 atccttcaca cccgaacggc cctccaccga gttcgcctcc agcgcacaat gcgtagcgga
    15961 acggtcgatc cggaacgcca ccggcggttc cggcgcatgg agggcgggat gccaggcgta
    16021 agcgtgattg cggcgcagca ccacgacgtc actgcccccg gccgcgcggt aggccaccgg
    16081 cgcgtcgcgc accacgttga actccaccac cgtgccgacc accgaagccg agtcgtcccc
    16141 gcccccccgt ttgcgactgg tcaaaatgac cccgttatgc cgcgagcgga tgaacgagtt
    16201 gtggcgcagc acattaccca gcgcccgcgg aaaggcgatc gggagattcg gcgcgtcacc
    16261 gctggtgacc aacacgccat cgctgcactc ttccgtgcgg ttcccctcgg ccagattcca
    16321 gaaaagcggg gagatgccgc ggttccaggt gcgcggcatg gacgaggcca acgtgctccc
    16381 gttggcgtag aaaaacagtc cctgcttgcg ttgacgcgcg atcgtgttcc ccgcgaccac
    16441 attctccacg cacgagatcc agagttggat gccggacatg ccgtccatcg tacggttccc
    16501 gacaatgagg ttacggtaga acatggtgcc gaccgccacg accgaaccgg cctgcggcgt
    16561 gacccgccac ggttggttaa gctgtagccg ccccccttca cgtgacacca cgcgccgcgt
    16621 ctgcccctga cccatgcccg cgaacaccgt gacgtaatac tcgctgatcg gcggttccgg
    16681 cgtgccgtcg tccacgtccg gcggccagaa cggcgtttcg cgccccgccg catcgtacgc
    16741 cagggcctca cgcttgacac tgcccagcag ggcgtccggt gtgggcggca gggttagcgg
    16801 cagcgtcaca ctttccgcat ccgcgccagc cagcggacca aagtacatcg tgcggtggtt
    16861 tgcctcgaat aaaatcatct cgcccacatt ctgatccgtg cccgccacgc caccgaaacg
    16921 cgcctgacct gcggcaccct ccgcctcggc gccgttccag gccagccagt tgtgcgtgac
    16981 cgagccgtgt cctgtcgaga gccagatcag ccgccgcgcg gtagggccgc ccgcctccgc
    17041 cccggccggt gaggcgaccg tattacgctc gatcacgcac cgctcgatcg gctccgtgcg
    17101 cccgaggatt gcaccctcgg aattgccgcc gtaacgggta accgaaacca gccggttcga
    17161 gacaaacacc gtatcgcgca cccccgagag aaagaggggg gtacgtcccc aaagctcatt
    17221 gccgcaaatc aggctgtgcc gcacctgccg aaggtagatg ccgcagttct ccgtctgctt
    17281 gccctcgcaa tccgccacgc gcacatcccg cacgcggcag ccgttcaccc attcggtctg
    17341 gcccgttgcc cgaatcgcga tcccctgatt gacttgaggc tgcccaagca ccgtcaggct
    17401 ctcgatggcc aggccgatct tggcaaagtg cggggcgagc tccttggcca gcgcctcggc
    17461 aaacatcacc tggttggcgg ccaggtcgag aaacggcagg ccgctgcccg caatggcgtt
    17521 gctgatgttc tgcagcacca tgccgcgcaa ctgcccttcg aggtcggcca cggggtagct
    17581 gtcgcgtgtg cccgagattt cggtgtggaa cagcttgggg tcggcgatac ggtaggcgta
    17641 gttgcca
//
LOCUS       DAOSRB010000041        17316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3418_length_17316_cov_8.210069, whole genome shotgun sequence.
ACCESSION   DAOSRB010000041 DAOSRB010000000
VERSION     DAOSRB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17316
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3418_length_17316_cov_8.210069"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..692
                     /locus_tag="PLZ74_03755"
     CDS             360..692
                     /locus_tag="PLZ74_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97469.1"
                     /translation="MTKKGSGVVWGRVCMKGWPMRVNKRGLFGLACLSLLFVAEVSAH
                     DIQVSRVRLTDWDEARQCVMVTFDLAWNNAWRDAGNWDAAWVFVKVKPAGSNDWRHAT
                     LSPEGRVR"
     gene            997..1323
                     /locus_tag="PLZ74_03760"
     CDS             997..1323
                     /locus_tag="PLZ74_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97470.1"
                     /translation="MSFPVFEAMMLICFGAAWPVSILKSWRSRTNRGKSLFFMVIVFA
                     GYIFGVCHKLFWQERVDGAVWLYLFNLVMVGTDLVLYYRNHRIDKAQADAPLYTAQRG
                     DLTQGR"
     gene            1335..2735
                     /locus_tag="PLZ74_03765"
     CDS             1335..2735
                     /locus_tag="PLZ74_03765"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97471.1"
                     /translation="MSLARVSRRRFAAAAGAVCAFPNLIPSSAFGQKAGRPAPSGRVN
                     VGLIGCGVQGITGNLNNFLHDDNTQVVSVCDPVSEAPLYGYRGQLQGGREPARWQVND
                     FYAKKNNKPGYDGCKVFADFRAMLEDKDLDAVCVATPDHWHALIGIMAARKGKHIFGQ
                     KPLANSVAEGRALCEAVKKHGVVFQTGAQGRSSDPNQRRAAELIRNRRLGKLQRIQLG
                     LPGGMPAWGKTAEQLSERPLTRPPAYVDFDLWLGPAPQSPYIPKLHLPMQWRFNFQYG
                     GGMVTDHGAHFIDILQWSLGMDESGPVAIENIRGEMDKPDSLYNVASRFSFEIVYEDG
                     TRVHTSTDFGENPSRFLRYFGENGRELYICGGKLETKPFDLIREKPRDGEVKLYVSSN
                     HERNFIEGILTGRPVISPCETGHRTITIAHLANIGLRLGRKDLKWNPRAERFVNDPEA
                     DAMLGVAYRAPWTLEG"
     gene            2832..3866
                     /locus_tag="PLZ74_03770"
     CDS             2832..3866
                     /locus_tag="PLZ74_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97472.1"
                     /translation="MIKVAIAGFGFMGRMHYGCWKKVPGVQVVALCDQDRRQFEQSAA
                     AAGNVQGADMSCDYGDAVLYDDFATMLDQARPDAVDITLPTFLHVSTAALALSKGIHV
                     LCEKPMALDAAACDELLAAWRAAPQGTELMIGQCLRFRGEYVYAKRLIESGEYGRVIA
                     ARFGRYSQAPGWTRDGGKSWFFDESRSGGVALDLHIHDTDMVHFLFGMPAAVTSSAYY
                     DSDGTMIHISTLYDVGGPSVVADGGWGMAKSFGFERGFQIAFERASLVMATNRQPEFC
                     LYPMDGGPFVPELPVSTDSYQNEINWFVDKLKGLPVEAVTTPEACRDSVKIVDAEKLS
                     ARGKKPVQIV"
     gene            3895..4716
                     /locus_tag="PLZ74_03775"
     CDS             3895..4716
                     /locus_tag="PLZ74_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOR97473.1"
                     /translation="MRQCKKWPIGICSWSLKTGVEEIGLVMTALGLEHMNLAIAPALA
                     PDGEAYLDAVRRQGWTITAGMMNYTYEDYSTLDTIRRTGGIAPDEHWPASGEAFGKAA
                     KITAELGSPYIMMHVGFLDHTDTAYAKKFYDRVRYLGDRAGEAGVSLLMETGQETAED
                     LQRFVETLNHPNVLLNFDPANLILYDKDEPIGAFRRLAPWVRHIHIKDGIRTATPGTW
                     GRKTFWGDGEVNAFAFLKALEDEGYDGALAIEYEANSLQERVAAITTAVRRLEAY"
     gene            4747..>5525
                     /locus_tag="PLZ74_03780"
     CDS             4747..>5525
                     /locus_tag="PLZ74_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOR97474.1"
                     /translation="MDKVKPQDLRVMIIGAHPDDPDITGGGLALNFVKVGATVKLVSM
                     CNGDKGHMTMSSEALAARRYQEAQRSKEILGVDEYRVLNHHDCELEVTLEMRKEVTRF
                     IREFAPHLVITHRTCDYHADHRAVGTLVMDSAYLLGVPLWCPETPIPAVKPAIYFLRD
                     AFTQPSEIRPDIVVDIEDEMDQLLTALCSHESQFFEWLVFDKGIAEPVPTSLEGRKEF
                     LKTYWMLPRKAADAVRFRARLTEVYGKERAKEIGFIEVYEL"
     assembly_gap    5526..5625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5787..7028
                     /locus_tag="PLZ74_03785"
     CDS             5787..7028
                     /locus_tag="PLZ74_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR97475.1"
                     /translation="MSEQQQASLRTRVVLSALYFLQYMALPVWFITLVPYAKSMGAQG
                     SMLAWMVSAMAIGTLASPFVGMVTDRFFAAQKVMAFLNVVSAVGLVCAGLQTKPLPLL
                     IILTVTMVFYMPTWSTINYIIFSHCSPPVFLQIRVFGVIGWIAAAAFSVVGDWIGVKV
                     NGTPLMFYCGAATVLVAGLLALLVPNTPPKARGQPMSLVDVLGLRAISMLRDPSFALF
                     IAIMFFSKVPFSLYFGYGGEFLADSGFKNPTLSMSFGQYSEIFFTLALASTIKKIGAK
                     NAMMIGLAALFLRYVFFWLGGPVGIYSGILIHGLIFGFFYVGGQIYVDSRADGAMRAQ
                     AQGFINFAAFGLGTFFANFVNRALIDGLRVTTTGADGVVHSNWSRIWLVSMILSAVLL
                     LIFALFCRSEDKPVSPDTQAA"
     gene            7166..11230
                     /locus_tag="PLZ74_03790"
     CDS             7166..11230
                     /locus_tag="PLZ74_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOR97476.1"
                     /translation="MRRRWGIGIAVVLLLGAGAGADEVYLTDLDISKTRQGWKEATRD
                     FNLFGARLQVAGRSYTNGMATCSPSELYVELNGARRFLAEVAVDDTSDQPDKRLVFQV
                     IGDGRLLWQSTPMRRGDRPVPVEVEVSAFTTLLLRVDPWRDGTAGDHADWLNARWVTD
                     GSRKPRAREPLNWPKAWLSTLDASFVTPDGAAVTSLPLRVAGVMYAEGLGMRVPSELC
                     VLRGSAARFAGKVGVDDAGADGSAVCMLYAGDRVLWSSGTMRCGEPARAFDIPIEEAG
                     QVRLVTTGDAGAQVDLVETLFTLTGRLRPCAAYNPAVYAKRPDWENPLVFRVGTEKPA
                     ATMWVFDSIRAARDATAREESPFFLSLDGVWKGHWVPHPDQRPQGFQQPDYPDGGWRD
                     LTVPGCVELAGLGTPLYKNIGYYFKVDPPFVLGEPDSRYTTHRERNAVTSYRRTFTLP
                     EDWEGRTVYLRFDGFASAMAVWLNGAKLGYAEDGRQGATFNVTAALRAGVNTLAVEVY
                     RLCDGSYMEDQDFWRLSGLCRPVYLWSVPKTHVRDVFVRTLPAVAGEFAGTWNLTVEA
                     EIAEGGDTDARLEAALFAYRFRNGFWNRTVAHGVAEAAGAGFRLNLPVVTPRLWSAEQ
                     PNLYTLVVTLRDARGRILQTIPQRVGFRQVEWDRGQLRVNGQPVLFKGVNRHEMDPDH
                     GYAVPRERMIQDITLMKRHNINAVRTSHYPNDPRWYDLCDAYGLYVMDEANLETHGLA
                     NTPRNPVVDPSYRAAALDRQMGMVERDKNHPSVIIWSLGNENEVDSDFFAEAYALIRA
                     RDPGRPIQNQRNGPRDFENTMYTRVRDLEAYGQRTDTERPFILCEYSHAMGNSSGNLA
                     EYWRVIESYPSLQGGFIWDFADQALRKPLPPERVRHGEETYFWAYGGDYGDFPNDDNF
                     NCNGLVQADRRPTPQLAEVRHVYQNVSVTARDVTRGLFTVHNKAFFTSLREVACRWTY
                     EENGEVIDSGSLGRLDVPPRGSLDFALPLSMVRRPVYAARVSTWNFRFLTLSDTPWAE
                     KGFEVARDQVVVPAEVQPTLMTATAGRAPVRLEETDREVTATGQDFDVRISKATGSIL
                     SWRVCGEEQILSPLEPDFWRAPTDNDRGNQMAARHAIWRDAAARREVRGVSLRQEVDG
                     NWRILVRLAFPSAGATSGTLHYSFTPAGQIRVAFKVTPAGVNLPSLPRVGMTLQIPLA
                     YDRVTWLGRGPHECYADRQASAFFGLYTCSLADLFFPYVEPQESGNRTDTFWVTFTDA
                     AGKGIRVTGDPKINFSARLYTTRELETRKHPWELTASGNGVVNLDYGQMGLAGENSWG
                     ARPWPEHQLSPGREYSYGFVLEPVSVIAAQRRLR"
     gene            11303..12460
                     /locus_tag="PLZ74_03795"
     CDS             11303..12460
                     /locus_tag="PLZ74_03795"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR97477.1"
                     /translation="MIRTVWLLVLVWFGAGLGCGTDSGQKALKQGLAAYKEKNYAAAV
                     THLNRAGKRLTGSVPLYYHLGLAHLHQGEIEPAQAAFAAALELAPGHAASKVCLGQIA
                     YLQNDLAKAQTCFDEALAVTEAPEERARILTALGLTEMGRNRNDLARLYLIRAQQAHR
                     AYAPAYYNLASLYRDAFNLREEALDQFELYVRLADRDDRHVEKAENNIKRLRLNLTRI
                     REEASEGVRRDPATAARLLQEGARLHADRQSAKAVKAFRDALAADPLTFSAAYGLAMA
                     YKAQGRRAEAMEAFRRAAQINPDHQDSYHQAAELAFQLKRYVEAGKLLDRAIARSPFQ
                     VASAELMARIRHAEGRLSEARAYGEYTLSLMHPNAKGRRAYEQWVRSLPAR"
     gene            12495..12992
                     /locus_tag="PLZ74_03800"
     CDS             12495..12992
                     /locus_tag="PLZ74_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOR97478.1"
                     /translation="MISILKGAGVWDYLAFAGVAFLVIRGFVRGCSGEMGRLVGTVTA
                     AAVGYFGFRPIANLVLASPVFGANPYAGRLVTFILMAVLCIAIWFGLSYLLTELIRLA
                     IAQPFDGILGGIIGGIKALVVVAAFVAWGLLNPKEEERVRLTEQSLTARWLAPLLKNI
                     TGPDG"
     gene            12999..13559
                     /locus_tag="PLZ74_03805"
     CDS             12999..13559
                     /locus_tag="PLZ74_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOR97479.1"
                     /translation="MSAYGQREEMEDAALMLQAGAGDNEAFAVLVRRHQKKLLNYFTR
                     TGVQYDCEDLVQQTFLRLYRYRNRYRPTAKFTTFLFLLARQVWVDELRRRKRQERLAK
                     GLAAEPHPEYAAPVAADAGASGGLDLAQALAGLPEGLRQVVELGVYQDLPYAEIAAIL
                     KIPVGTVKSRMFTALARLRAHWEEKP"
     gene            13556..14668
                     /locus_tag="PLZ74_03810"
     CDS             13556..14668
                     /locus_tag="PLZ74_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOR97480.1"
                     /translation="MNLAAHLAALPPAEASGLRAVAERLRQLPEREPSADLTERILRA
                     VNTEHSRPPFRAPKLWRVAAVLVATLALVALYLRFGVPAVRDAQADDGSCWIAAAQEA
                     DGTWQPARHGGAEAYRPALTALSALALDRADGRKYADPIGRACKALAALQNEDGAFGG
                     NGRERYYNHAMTTFALAALASDRPGLKPVLERALAFSRASQTVEGGWDYEPGSDGNAA
                     LTSWQVRALAEMRRIGFAEAEIPLRKGLRWLRGTLREDGHVAYHRGTRNPSECLDALT
                     AHTLITAGGAYAGLADLGRQVAGRLNPAEPEDVADCYRDYAKTLAFAAAGAAEQAARV
                     SRQMIAHHQAGARDQWERVGGRLYTAALTRLAVGAL"
     gene            14724..16985
                     /gene="rnr"
                     /locus_tag="PLZ74_03815"
     CDS             14724..16985
                     /gene="rnr"
                     /locus_tag="PLZ74_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOR97481.1"
                     /translation="MNKFDKEKAAIQDLFESGPERAWTAKEISRTLGLRGKQINRLHD
                     RLGEMVRDGVIVQIRRGNAYTLGKQADLVTGPLRTVRNGAGYVTERESGKSIWVESED
                     LGTALPGDLVTIRLYRNGVERKGKVIRIVERSKRDIVGTLMTTGRFLHVVPLNPVYRK
                     NFYVPAANGAREGDRVVVRFTDWKNRYVAPEGEIVDVIGPADKPSLDTLVVMKQFDLP
                     EEFPPEVIDEAEKVARRLSRPGKREDLRSRYIITVDPATARDFDDAVSLESDEHGNRV
                     LGVHIADVSHFVRPGAPLDREARERGTSVYLVDKVIPMLPEQLSNGVCSLRPDEDRLT
                     FSAFMTFDASGRMVARRFAKTRIRSKLRLSYEQAMDILSDRKPKGLASVPKPARALLK
                     GARRLAEQLRARRFSMAALDLEVPEVEVVLDEQARMTGLVTRPYDASHQLIEECMIAA
                     NEAVATELWTRGIKILARLHEPPDGEKLEELQASLAILGFRPGDLSNPKHLARFLTDT
                     REHPLRYHAHMMVLRSMKRAVYSAEKIGHFGLAKTYYAHFTSPIRRYPDLLLHRQLAD
                     FLDGKGGRMPQHELVQTAFLATEREQVADDASRQLIEIKKFRFLQQQLDERKPIPYRA
                     VVVKCTNYGVFIDIPGLALGGLVHISNLSRQFVRFNPANESLSAGQTVYRVGTVLQVQ
                     VASVDFNQRHADFMLVGGEASGERGDGTEQPRKGGARRSAGEKRGRQSDTAGRRPRGE
                     PRPAKSGRRRKGR"
BASE COUNT         3140 a         4837 c         5820 g         3419 t
ORIGIN      
        1 cccctcgtgg catgggcgtc ccgcccatga aacacgggca agatgcccgt gccatctgtc
       61 tgtgtccacc gccgcgacga ctccgaaaaa caccaagctc ctcatgatct ctccggcctt
      121 gaggagtacc cccctcctgt cggcagttgc ggcgcatacg gaacagcgtc aaaataatag
      181 cgaacggccg tcttgtcaat cgcaacagcc acatgatgcc attcgccatc gtccaagttc
      241 gcggtttcgt gaacggtgac ttgcgatccg caacattcga cctgcggcgt ttggattctt
      301 tggcaggtct gacaattatt cataaccttc tgcgatcggt tttgtggtac agttatgtca
      361 tgaccaaaaa aggttccggt gtggtctggg gccgggtctg catgaaaggg tggccgatgc
      421 gagtcaacaa acgagggtta ttcgggttgg cgtgtttatc ccttttgttc gtggcggagg
      481 tttccgcgca tgacattcag gtctcccgcg tgcggctgac cgactgggac gaggcgcgtc
      541 agtgcgtgat ggtgacgttc gatcttgcct ggaacaacgc ctggcgggac gcggggaact
      601 gggacgcggc ttgggtcttc gtcaaagtga aacccgcagg atcgaatgac tggcggcatg
      661 cgacgctctc gccggagggt cgggtacgct gacaaccgcc ggcgaggccg caaatgccac
      721 atggccgaac gggatgggac gccgcggcgg cggatgcaat tcgaataaca cggtatcgga
      781 ccgctatctg gcgtcgcatg tggataatac acggtatttt ccgataggct tccgatgtgt
      841 ccgtacggcg ccgtgaggcc tccaagtcaa aacaggcgga agagatgtat gctggaggta
      901 gggacggttc cccgaaccct gtcagttgca aagggcaaaa cggatttata aaatgaccag
      961 atttaagtga acaagtacga gtactgaaag gagcgtatgt cttttccggt ttttgaagcg
     1021 atgatgttga tttgtttcgg cgcggcttgg ccggtttcca tcctgaagtc gtggcggtcg
     1081 cggaccaaca gggggaaaag cctttttttc atggtgattg ttttcgccgg ttacattttc
     1141 ggggtgtgcc acaagctgtt ttggcaggag cgggttgacg gcgcggtctg gctctatctg
     1201 ttcaatctgg tcatggtcgg caccgacctc gtcctctatt accggaatca tcggattgac
     1261 aaagcgcagg cggatgcgcc attgtacacg gcacagcgtg gggatctgac tcaagggagg
     1321 taagggttga gggtatgagc ttggcacggg tttcacgacg ccgtttcgcg gcggccgcag
     1381 gcgcggtctg cgcgtttccg aatcttattc cgtcttcggc gttcgggcaa aaagcggggc
     1441 gtcccgcacc ctccggccgc gtcaatgtgg gcttgatcgg gtgcggggtt caggggatca
     1501 ccggaaacct caacaacttc ctgcatgacg ataacacgca ggtcgtttcc gtctgcgatc
     1561 cggtttcgga ggcgccgctc tacggatacc gtggccagct tcagggcggc cgggagccgg
     1621 cgcggtggca ggtgaacgac ttctacgcca aaaagaacaa caagcccgga tacgacgggt
     1681 gcaaggtgtt cgccgatttc cgcgcgatgc tggaggacaa ggatctggac gcggtgtgcg
     1741 tggccacgcc ggaccattgg cacgcgttga tcggcatcat ggccgcgcgc aagggcaagc
     1801 acatcttcgg tcagaagccg ctggcgaaca gcgtggcgga gggccgcgcg ttgtgcgagg
     1861 cggtgaaaaa acacggcgtg gtctttcaga ccggggcgca gggacgctcc agtgacccga
     1921 accaacgcag agcggccgag ttgatccgca accggcggtt gggcaagctg cagcggatcc
     1981 agctcgggct tcccggcggc atgccggcct ggggcaagac cgcggagcag ctcagcgagc
     2041 ggccgctgac acggccgcct gcgtacgtcg acttcgacct gtggctgggg cccgcgccgc
     2101 aaagccccta catccccaag ctgcacctgc ccatgcagtg gcgcttcaac ttccagtacg
     2161 gcggcggcat ggtgaccgac cacggcgcgc acttcatcga catcctgcag tggtcgctgg
     2221 gcatggacga gtcgggcccg gtcgcgatcg agaacatccg gggcgagatg gacaagccgg
     2281 acagcctcta caacgtggcc tccaggttca gcttcgagat cgtgtacgag gacggcacgc
     2341 gcgtgcacac ctccacggac ttcggcgaga acccgtcacg gttcctgcgc tatttcggcg
     2401 agaacggccg tgagttgtac atttgcggcg gcaagctgga gaccaagccg ttcgatctga
     2461 tccgcgagaa gccgcgtgac ggcgaggtga agctctatgt gagcagcaac cacgagcgca
     2521 acttcatcga gggcatcctg accggcaggc cggtgatctc cccctgcgag acggggcacc
     2581 ggaccatcac catcgcgcat ctggccaaca tcggcttgcg tctgggcagg aaggacctga
     2641 agtggaatcc ccgcgccgaa cgcttcgtga acgatcccga ggcggacgcg atgctgggtg
     2701 tggcgtaccg cgcgccgtgg acgcttgagg gctgatgcgg atgcgtgtcg tgtccaaaca
     2761 ggcagaagtc ttctgttcaa cggttgacgc gaagcgtttg agcgataagg gataacgaaa
     2821 gagcaggcag tatgatcaaa gtagcgattg cgggtttcgg tttcatgggt cggatgcact
     2881 acggatgctg gaaaaaagtg cccggggtgc aggtggtggc gttgtgcgat caggatcgcc
     2941 gacagttcga gcagtccgcc gccgcggccg gaaacgttca gggtgccgat atgtcgtgcg
     3001 attatggcga cgctgtcttg tatgacgact tcgcgaccat gctggatcag gcccggccgg
     3061 atgcggtgga catcaccctg ccgacgtttc tgcatgtctc gaccgccgcg ttggcgttgt
     3121 ccaaggggat ccatgtgctt tgcgagaagc cgatggcgct tgatgccgcg gcgtgcgacg
     3181 agttgcttgc ggcctggcgc gccgcgccgc aaggcacgga gttgatgatc ggccagtgtc
     3241 tgcgcttccg cggcgaatat gtgtatgcca agcgcttgat cgaaagcggt gagtatggcc
     3301 gcgtgatcgc cgcgcgcttc gggcggtaca gccaagcgcc cggctggacg cgtgacggcg
     3361 gcaagagctg gttctttgac gagtcgcgca gtggcggggt ggcattggat ctgcacattc
     3421 acgataccga tatggttcac ttcctgttcg gcatgccggc ggcggtgacc agctccgcgt
     3481 attatgacag tgatggcacc atgatccaca tttccacgtt gtatgatgtc ggcgggccat
     3541 ccgttgtggc ggatggcgga tggggtatgg cgaagagctt tgggtttgag cgcggtttcc
     3601 aaatcgcctt tgagcgggcc tcactggtca tggccaccaa ccgtcagccg gagttctgcc
     3661 tgtatccgat ggatggcggg ccgtttgtgc cggagttgcc ggtttctacg gattcgtatc
     3721 agaacgagat caactggttc gtggacaaat tgaagggcct tccggtcgag gcggtgacca
     3781 ccccggaggc atgccgcgat tcggtaaaga ttgtcgatgc cgaaaagctt tccgcgaggg
     3841 gcaagaagcc ggtgcagatc gtttagtgtc ttgaacgatg gaagggggta tgcgatgaga
     3901 cagtgcaaaa aatggccgat cgggatttgc agttggtcct tgaagaccgg tgttgaggag
     3961 atcggtttgg tgatgaccgc tttagggcta gagcacatga atctggccat tgccccagcg
     4021 ctcgcgcccg atggagaagc ttatctggat gctgtgcgtc ggcaaggctg gacaatcaca
     4081 gccggcatga tgaattacac atacgaggat tattcaacgc tcgatacgat ccggcggaca
     4141 ggcggcatcg cgccggatga gcactggccc gccagtggcg aggccttcgg aaaagccgcg
     4201 aagatcacgg cggagctggg ttcaccgtat atcatgatgc acgtcggttt tcttgatcac
     4261 acggacaccg cctacgccaa gaaattttat gaccgggtgc ggtatctggg tgatcgcgcg
     4321 ggcgaggccg gcgtgtcgct gctgatggag accgggcagg aaaccgccga ggatttgcag
     4381 cgctttgtgg aaacgctgaa ccatcctaac gtgttgctga actttgatcc cgccaatctc
     4441 attctatacg acaaggatga gccgatcggg gctttccggc gactggcgcc gtgggtccgc
     4501 catatccaca tcaaagatgg gataagaacc gccactcccg ggacatgggg acgtaagacg
     4561 ttctgggggg atggagaggt caatgccttc gcttttttaa aggcattgga agatgagggg
     4621 tatgacggag cgttggcgat tgaatacgaa gcgaacagtc tgcaagagcg ggtggcggcc
     4681 ataacgaccg cagtgcgtcg gcttgaagca tattgaaaaa gcgaaacgcg gtaatcgagg
     4741 tgggacatgg ataaagtcaa accgcaagat ttgcgggtga tgatcatcgg ggcgcatccg
     4801 gatgaccccg acattacggg cggaggcttg gccctgaact ttgtgaaggt cggggcaacg
     4861 gtgaagctcg tctcaatgtg caacggagac aaggggcaca tgacgatgtc gtcggaggca
     4921 ctcgccgcgc ggcggtatca ggaggcacag cggtcgaagg agatcttggg tgtcgacgag
     4981 taccgggtcc tcaatcacca cgactgcgaa ctggaagtga cgctcgagat gcgtaaagag
     5041 gtgacgcgtt ttatccgaga gttcgcgccg cacttggtta ttacgcaccg cacgtgcgac
     5101 taccatgccg accacagggc ggtcggaacc ttggtgatgg actcggccta tctgctgggg
     5161 gtgccgcttt ggtgcccgga aacgcccatt ccggcagtca agcccgcgat ctatttcctg
     5221 cgcgacgcat tcactcagcc gtcggagatc cggccggaca tcgttgtcga tatcgaggac
     5281 gagatggatc agttgctcac ggccctctgt tcacacgagt cgcagttttt cgagtggctg
     5341 gttttcgata aggggattgc cgagccggtt cccacctctt tggaggggcg caaggagttc
     5401 ctcaagacgt actggatgct cccgcgcaaa gcggccgatg ccgttcggtt ccgtgcccgc
     5461 ctgacggagg tttatggcaa ggaaagggcg aaggagatcg gctttatcga ggtctatgag
     5521 cttagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttat ggcaaggaaa
     5641 gggcgaagga gatcggcttt atcgaggtct atgagcttag tgagtatggg tatcagccga
     5701 cagctgatga ggtgggctgg ctgttcccgt ttgttccggg gtgtgcttcg gcaacgggtg
     5761 tgccgaaacg ataaaaggag ggaaggatgt ctgaacaaca gcaggcttcg ttaaggacgc
     5821 gggtggtttt gagcgcattg tattttttgc agtacatggc tctgccggtc tggttcatca
     5881 cccttgtccc atacgccaaa agcatgggcg ctcaaggctc catgctggct tggatggtca
     5941 gcgccatggc gatcgggacg ctggcctccc cgtttgtcgg catggtcacc gaccggtttt
     6001 tcgccgcgca gaaggtcatg gcgtttctga atgtggtgtc ggccgtcgga ttggtttgcg
     6061 ccggactaca gacgaagccg ctgccgctgc tgatcatcct gaccgtgacc atggtgtttt
     6121 acatgcccac ctggagcacg atcaactaca tcatcttctc gcactgctcc cccccggtgt
     6181 ttctgcagat ccgcgttttc ggcgtgatcg gctggatcgc cgctgccgcc ttcagcgtgg
     6241 tgggcgactg gatcggggtc aaggtcaacg gcacgccgct gatgttctat tgcggcgcgg
     6301 cgacggtgct ggtggccggt ctcttggcct tgttggtgcc gaacacgccg ccaaaggcgc
     6361 gtggtcagcc tatgagtctg gtggatgtcc tcgggctccg cgccatctcc atgctgcggg
     6421 atcccagttt cgcgctgttc atcgcgatca tgttcttctc aaaggtcccc ttctctctct
     6481 atttcgggta cgggggcgaa ttcctggcgg actcggggtt caagaacccc accctgtcca
     6541 tgagtttcgg acagtacagc gagatctttt tcacgctggc gctcgcttcg accatcaaga
     6601 aaatcggcgc gaaaaatgcg atgatgatcg ggttggccgc gctgtttctg cgttatgtct
     6661 ttttctggtt gggtggcccc gtggggatat acagcgggat cctgatccac gggctcatct
     6721 tcggattctt ttacgtgggg gggcagatct atgttgattc ccgggcggac ggggcgatgc
     6781 gggcgcaggc gcagggattc atcaacttcg ccgcgtttgg tctcggcacg ttctttgcca
     6841 acttcgtcaa ccgtgcgttg atcgacgggc tgcgcgtgac cacaacggga gcggacgggg
     6901 ttgtccacag caactggagc cggatttggc tcgtctccat gatcctctcc gcagtgttgc
     6961 tgcttatttt cgctctcttc tgccgctcgg aggataaacc tgtgtctccg gacactcagg
     7021 ccgcatgagt tccgcgcact tgcgccacca accggttgcc aaaaggtccc ttgccgcaca
     7081 agagggggtt gatctacagt acggtaatgg atagcggacg cggtgcgacg cggaaggaaa
     7141 cggtatcgcg gaaagggagc aggggatgag acgacgttgg gggatcggga ttgcggtcgt
     7201 gttgttgctg ggcgcggggg caggtgcgga tgaggtgtac ctgacggatc tcgacatctc
     7261 gaagacccgt cagggctgga aagaggcgac gcgggatttc aacctcttcg gcgcgcggtt
     7321 gcaggtggcg ggacggagct acaccaacgg gatggcgacc tgttcgcctt ccgagctcta
     7381 tgtcgagttg aacggggcgc gccggtttct cgccgaggtg gcggtcgacg ataccagtga
     7441 tcaaccggac aagcgcctcg ttttccaagt gatcggcgat gggcgcctgc tgtggcagag
     7501 cacgccgatg cggcgcggcg accggccggt accggtggag gtggaggtgt cagcgttcac
     7561 cacgctcctg ctgcgtgtgg atccgtggcg ggacggtacg gccggggatc atgccgattg
     7621 gctgaacgcg cggtgggtga cggacggttc gcgtaagccg cgcgcgcggg aaccgctgaa
     7681 ctggccgaaa gcctggctct ccacgctgga cgcatcgttt gtgacacccg atggcgcggc
     7741 ggtgacctct ctgccgttgc gggtggcggg tgtcatgtat gcggaaggcc tcgggatgcg
     7801 ggtgccttcg gaactgtgtg tgctccgggg ttcggccgcg cgctttgccg ggaaagtcgg
     7861 tgtggacgac gctggcgcgg acggaagcgc ggtctgcatg ctctatgcgg gggatcgggt
     7921 attgtggagc agcggcacca tgcggtgcgg tgagccggcc cgggccttcg acatccccat
     7981 cgaagaggcg ggacaggtac ggctggtgac cacaggcgac gcgggcgcac aggtcgattt
     8041 ggtggagacg ctcttcacgc tgacgggtcg cctacggccc tgcgcggcct acaatccggc
     8101 cgtttatgcg aagcgtcccg attgggagaa tccgctcgtg ttccgggtcg gcacggaaaa
     8161 accggcggcc acgatgtggg tcttcgactc catccgtgcg gcgcgtgacg ctacggcacg
     8221 cgaggagtcc cctttctttc tctcgctcga cggcgtgtgg aaaggccatt gggtaccgca
     8281 tccggaccag cggccccagg ggtttcagca gccggactat ccggatggcg gttggcgcga
     8341 tctgacggtg ccgggatgcg tggaactcgc gggactggga acgccgctct ataaaaacat
     8401 cggctactac ttcaaggttg accccccgtt cgtgttgggc gagccggatt cgcggtacac
     8461 cacccaccgc gagcgcaatg cggtgacctc ctaccgccgc acgttcaccc tgccggagga
     8521 ttgggaggga cgcaccgtgt atctgcggtt tgacggcttc gcctcggcca tggccgtatg
     8581 gctcaacggc gcgaaactgg ggtatgcgga ggatgggcgt cagggcgcga cgttcaacgt
     8641 gacggccgcg ctccgggcgg gggtgaacac gctggcggtg gaagtatacc gcctgtgcga
     8701 cggctcctac atggaggatc aggatttctg gcgtttgtcg ggtctctgcc gcccggtcta
     8761 cctctggtcc gttcccaaaa cccatgtgcg ggatgtgttt gtgcggactc tgccggcagt
     8821 ggcgggggag tttgcaggga cttggaatct gacggttgag gcggagatcg cggagggcgg
     8881 cgacacggac gcgcgtctgg aggcggcgtt gttcgcctac cggttccgga acggtttctg
     8941 gaaccggacc gtggcgcatg gcgtggcgga ggcagccggt gcgggattcc gcctcaacct
     9001 gccggtggtg acaccgcgtt tgtggtcggc ggaacaaccc aacctctata ctctggtagt
     9061 gactttgcgc gacgcgcgcg gacggattct gcagacgatc ccgcagcgcg tcgggttccg
     9121 gcaggttgaa tgggatcgcg ggcagctccg ggtgaacggc cagcccgtgc tgttcaaagg
     9181 cgtgaaccgc catgagatgg atccggacca cgggtacgcg gtaccgcggg agcgcatgat
     9241 tcaggacatc acactgatga agcggcataa catcaacgcg gtccggacca gccactatcc
     9301 caatgacccg cgttggtacg acctgtgcga tgcgtacggg ctgtatgtga tggacgaggc
     9361 caatttggaa acgcacgggt tggccaacac accgcgtaat cccgtggttg acccctccta
     9421 ccgcgcggca gcgctggatc ggcagatggg catggtcgaa cgcgacaaga atcatccctc
     9481 agtcatcatc tggtccctcg gcaacgagaa cgaggtggat tccgatttct ttgcggaggc
     9541 ctacgccctg atccgggcgc gcgatccggg acggccgatc cagaatcagc gcaacgggcc
     9601 gcgcgatttt gaaaacacca tgtatacgcg tgtgcgggat ctggaggcct acggtcagcg
     9661 aaccgatacg gagaggccct ttatcctgtg cgagtattcg catgctatgg gcaattccag
     9721 cgggaatctc gccgagtact ggcgggtgat cgagtcctac ccctcgctgc aaggcgggtt
     9781 catctgggat ttcgcggatc aggccctgcg caagccgttg ccccctgagc gtgtgcggca
     9841 tggcgaggag acgtattttt gggcatacgg cggcgattac ggggattttc cgaacgacga
     9901 caacttcaac tgtaacggat tggtgcaggc ggaccggcgg cccacgccgc aactggcgga
     9961 ggtgcggcac gtttatcaga acgtctcggt gacggcgcgc gatgtgacgc gcgggctttt
    10021 tacggtccac aacaaggcgt tcttcacgtc gctgcgggag gtggcgtgcc ggtggacgta
    10081 cgaggagaac ggcgaggtga tcgacagcgg gagtctcggg cgactcgatg tgccgcctcg
    10141 gggatcgctg gatttcgctc tgccgctctc tatggtgcgc cgcccggttt acgccgcgcg
    10201 ggtcagcact tggaatttcc ggttcctgac gctcagcgac accccttggg cggagaaggg
    10261 cttcgaggtg gcgcgcgatc aggtggtggt gcccgccgag gtgcagccga ccctgatgac
    10321 cgcgaccgcg ggacgtgcgc cggtgcggct ggaggagacg gaccgggagg tcaccgcgac
    10381 gggacaggac tttgacgtgc ggatcagcaa ggccacgggg tcgattctct cgtggcgggt
    10441 gtgcggtgag gagcagatcc tgtcgccgct ggagccggat ttctggaggg cgccgaccga
    10501 caacgaccgc ggcaatcaga tggcggcgcg ccatgcgatc tggcgcgatg cggcggcgcg
    10561 gcgcgaggtt cgcggcgtca gtctccggca ggaggtggac ggcaactggc gcattctggt
    10621 tcgtctggcc tttccctcag cgggcgcgac ttccggcaca ctccattact cgttcacccc
    10681 ggcggggcag atccgcgtgg cgttcaaggt gacgcctgcg ggcgtgaacc tgccatcgtt
    10741 gccgcgtgtg gggatgacgc tgcagatccc gctcgcgtat gaccgggtga cgtggctcgg
    10801 gcgcggaccc cacgaatgtt atgctgatcg ccaagcttcg gcctttttcg gcttgtacac
    10861 ctgttctctg gccgatctct tctttccgta tgtcgagccc caggagagcg gcaaccgcac
    10921 ggataccttt tgggtgacct tcacggatgc ggcaggcaag gggatccggg tcacaggcga
    10981 tcccaaaatc aatttcagcg cgcggctcta cacgacgcgt gaactcgaaa cgcgcaaaca
    11041 cccctgggaa ctcacggcca gcggtaacgg ggtggtgaat ctggattacg gacagatggg
    11101 tttggccggc gagaacagtt ggggggcgcg tccttggccc gaacaccaac tctcgcccgg
    11161 acgcgaatac agctacggtt ttgttctgga acccgtttcc gtgattgcgg cgcaacggcg
    11221 tcttcggtaa tatacaaacg gttttactgt tcccggtccg gtcgtcgggt ggcggctgcg
    11281 gggcgtggtg aggtggtccg ttatgattcg cacagtatgg ctgctcgttt tggtttggtt
    11341 cggcgcagga ctcgggtgcg gcaccgattc cgggcagaaa gctttgaagc aggggctggc
    11401 cgcgtacaaa gagaagaatt acgcggcagc ggtcacgcat ctgaaccggg ctggcaagcg
    11461 gctcaccgga tccgttccgt tgtactatca tctggggctc gcgcatctgc atcaggggga
    11521 aatcgaaccg gcgcaggcgg ccttcgcggc ggcgctggag ttggcaccgg gtcatgccgc
    11581 gtccaaagtc tgtctggggc agatcgctta tctgcagaat gatctggcca aggcgcagac
    11641 ctgtttcgac gaggctctcg ctgtgaccga ggcaccggag gagcgggccc ggatcctgac
    11701 cgcgctcggt ttgaccgaga tggggcgtaa ccggaatgat ctcgcgcgtc tttatctgat
    11761 ccgcgcgcag caggcccacc gcgcctacgc tccggcgtac tacaatctcg ccagtctcta
    11821 ccgcgatgcc ttcaacctgc gggaagaggc gctggatcag ttcgaactgt atgtgaggct
    11881 ggctgaccgg gatgaccgcc atgtcgaaaa agcggagaac aacatcaaac ggctgcgcct
    11941 caacctcacc cggatccggg aggaggcgag cgaaggtgtg cggcgcgatc cggctactgc
    12001 ggcacgcctg cttcaggagg gcgcccggct gcatgccgac cggcagtcgg ccaaggcggt
    12061 caaggccttc cgtgacgcgc tggccgccga tcctctgacg ttcagcgcgg cgtacgggct
    12121 ggccatggca tacaaggctc aaggtcggcg cgccgaggcc atggaggcct ttcggcgtgc
    12181 cgcgcagatc aaccccgatc atcaggacag ctatcatcag gccgctgaac tggctttcca
    12241 attgaaacgc tacgtcgagg cggggaagct gttggaccgc gcgatcgcgc gcagcccctt
    12301 tcaagtcgcc agcgctgaat tgatggcgcg gatccggcat gcggagggaa ggttgtccga
    12361 ggcgcgcgcg tacggtgagt ataccctctc gctgatgcat ccgaacgcga aggggcggcg
    12421 cgcgtacgaa cagtgggtgc ggtcgctgcc ggcccgatga gtacacgggt acggcaggcg
    12481 gacgggacgg tgttatgatc tcaatcctca aaggggcggg cgtttgggac tatttggcgt
    12541 ttgccggtgt ggcctttctg gtgatccgcg ggtttgtgcg gggctgttcc ggtgagatgg
    12601 gacgtctggt gggaaccgtg accgccgccg cggtcgggta tttcggattc cgcccgatcg
    12661 ccaacctggt gctcgcctcg ccggtgttcg gcgccaaccc ctatgccgga cgtctggtca
    12721 cctttatttt gatggcggtg ctctgcatcg cgatctggtt cggattgagt tatctgctca
    12781 ccgaactcat ccgcctggcc attgcccagc cgtttgacgg cattctgggc ggcatcatcg
    12841 gcggcatcaa ggcgctggtg gtggtcgcgg ccttcgtggc ttggggactg ctcaatccta
    12901 aagaggagga gcgcgtccgg ttgacggaac aatcgctgac cgcacggtgg ctcgccccgc
    12961 tgctgaaaaa tatcaccggt cccgacggat aggggcgtat gagcgcatac gggcaacggg
    13021 aagagatgga agatgccgcc ctcatgctgc aggcgggcgc cggagacaac gaagcctttg
    13081 cggtgctggt ccgtcggcat cagaaaaagc tcctgaacta tttcacgcgt accggcgttc
    13141 aatatgattg tgaggatttg gttcagcaga ctttcctgag gctataccgt taccgtaacc
    13201 gctatcggcc gaccgcgaag ttcacaacct ttctctttct gctcgcgcgg caggtgtggg
    13261 ttgatgagtt gcgtcggcgc aagcgtcaag aacggctggc caaaggtctg gcggcggagc
    13321 cccacccgga atacgctgcg ccggttgcgg ccgacgcggg cgcgtcgggt ggtctggatt
    13381 tggcgcaggc gctggccgga ttgcccgagg gtctccggca ggtggtcgaa ctcggtgtct
    13441 atcaggatct gccgtacgcg gaaatcgcgg cgattctgaa aatcccggtg gggaccgtca
    13501 agtcgcgcat gtttaccgcg ctcgcgcgat tgcgggcgca ttgggaggag aaaccatgaa
    13561 tctcgccgca catttggctg cgttgccgcc tgcggaggcg agcgggctcc gcgctgtcgc
    13621 ggaacgtctg cgtcaactgc cggaacgcga accgtcggcc gacctgacgg aacggatcct
    13681 gcgtgctgtg aacacggaac actcccgtcc gcctttccgt gcgcccaagc tgtggcgggt
    13741 cgcggccgtg ctggtcgcga cgctcgcgct ggtcgcgctc taccttcgtt tcggcgtccc
    13801 ggctgtgcgc gacgcgcagg cggacgacgg atcgtgctgg atcgccgcag cgcaggaagc
    13861 ggacggcaca tggcagcccg cccgacatgg cggcgccgag gcttaccgtc ccgcgctgac
    13921 cgccctctcc gccctggcgc tcgaccgtgc ggatggacga aagtacgccg acccaatcgg
    13981 gcgcgcgtgc aaggcgctcg ccgccctcca gaatgaggac ggtgcctttg gcggcaacgg
    14041 acgcgagcgg tattacaacc atgccatgac gaccttcgcg ctggccgcgc tggcttccga
    14101 caggcccggc ctgaaaccgg ttttggagcg tgcgctggcc ttcagccgcg ccagccaaac
    14161 ggtcgagggc ggttgggatt acgaacccgg ctccgacggg aacgccgctc tgacctcgtg
    14221 gcaggtgcgc gcgttggcgg agatgcggcg gatcggattc gcggaggcgg aaatcccgct
    14281 gcgtaaaggg ttgcgctggc tgcgcggcac actgcgcgag gacggacatg tggcgtatca
    14341 ccgggggacg cgcaacccgt cggagtgtct ggacgcgcta accgcccaca ctctgatcac
    14401 agcgggcggt gcctatgccg gcttggctga cttgggcaga caggttgcgg gtcggctgaa
    14461 tccggccgaa ccggaggatg ttgcggattg ttatcgggat tatgccaaaa cgttggcgtt
    14521 cgccgcagcc ggtgcggcgg aacaggccgc acgggtttcc cgtcagatga ttgcgcacca
    14581 tcaggccggc gcgcgcgatc aatgggaacg tgtcggcgga cggctctata ccgccgccct
    14641 gacgcgcctc gcggtcggcg ccctgtagtg acgcatgttt tttctacaga cgcgggtgcg
    14701 gcggatcagg tacactgact ccgatgaaca aattcgacaa agagaaagcc gccattcaag
    14761 acttgttcga atccgggccg gaacgggctt ggacagccaa ggagatcagc cggactctgg
    14821 ggttgcgagg caagcagatc aatcgactcc acgaccggct gggcgagatg gttcgtgatg
    14881 gcgtgatcgt gcagatccgc cgcggtaacg cctacacgct cggtaaacag gcggatctgg
    14941 tcacaggccc gttgcgcaca gtgcggaacg gcgcggggta cgtcacggaa cgcgagtcgg
    15001 gaaaaagcat ctgggtcgaa tccgaagacc ttggcactgc gctacccggt gatctcgtga
    15061 cgatccgtct ctaccgcaac ggcgttgaac ggaagggcaa ggtcatccgg atcgtcgagc
    15121 ggtcgaagcg ggatatcgtg ggcacgctga tgacgaccgg acgctttctc catgtggtgc
    15181 cgctgaaccc ggtgtaccgt aagaattttt atgttcccgc tgccaacggt gcccgggagg
    15241 gcgatcgtgt ggtggtgcgc ttcacggact ggaaaaaccg gtacgtcgca cccgaggggg
    15301 agattgtgga cgtgatcggg cccgcggata agccgtcgct cgacacgttg gtggtgatga
    15361 agcagtttga cctgcccgaa gagtttccgc ccgaggtgat cgacgaagcg gaaaaagtgg
    15421 cgcggcgtct gagccgcccc ggaaaacgcg aggatctgcg ctcgcgctac atcatcacgg
    15481 tggatcccgc cacggcgcgt gacttcgacg atgccgtctc gttggagagc gacgaacacg
    15541 gcaaccgggt gctgggggtc cacatcgcgg atgtcagcca tttcgtccgg ccgggggcac
    15601 cgctggaccg ggaggcgcgt gaacgcggaa ccagcgtgta tctggtggac aaggtgatcc
    15661 ccatgctgcc ggaacagctt tccaacggcg tgtgcagtct gcggccggat gaggaccggc
    15721 ttaccttttc ggcgttcatg accttcgacg cctcgggccg catggtcgcc cgccgtttcg
    15781 ccaaaacgcg gatccgctcc aaactgcggt tgagttatga gcaggccatg gatatccttt
    15841 cggaccggaa accgaaaggg ctggcctctg tgcccaaacc ggcccgtgcg ctcctgaaag
    15901 gcgcgcgacg gctggctgag caattacgcg cgcggcggtt ctccatggcg gcgttggatt
    15961 tggaggtgcc cgaggtcgag gtggtgctgg acgaacaggc gcgcatgacc ggtctggtga
    16021 cgcgccccta cgatgcgtcc catcagctta tcgaggagtg catgatcgcg gccaatgagg
    16081 cggtcgcgac cgaattgtgg acacgcggca tcaaaattct ggcgcggctg catgagccgc
    16141 cggacggaga gaaactcgaa gagttgcagg ccagtctggc gatcctcggt ttccgccccg
    16201 gggatctttc aaaccccaag catcttgccc ggtttctgac ggatacgcgg gaacacccgc
    16261 tacgctatca cgcccacatg atggtgctgc gcagtatgaa gcgcgccgtc tactccgccg
    16321 aaaagatcgg ccatttcgga ttggccaaaa cgtactatgc gcactttacc tcgccgatcc
    16381 ggcggtatcc cgatctactc ctgcaccggc aactcgccga ttttctggac ggcaagggcg
    16441 gacgaatgcc gcagcacgaa ttggtgcaga ccgcgtttct ggcgacagag cgcgaacagg
    16501 tcgcggatga cgcctcccgc caactgatcg agatcaaaaa gttccgtttt ctccagcagc
    16561 aactggacga gcggaagccg atcccgtacc gcgccgtcgt ggtgaagtgc accaactacg
    16621 gggtgttcat cgatatcccc gggttagctc tgggcggctt ggtccacatc tccaacctct
    16681 cccggcagtt tgtccgcttt aatcctgcga acgaaagcct gagcgcgggt cagacggttt
    16741 accgtgtcgg aaccgtgcta caggtgcagg tggcgtctgt cgacttcaat cagcgccacg
    16801 ctgatttcat gctggtcggc ggggaggcgt ccggggagcg cggcgatgga acggagcagc
    16861 cgcgcaaagg cggtgcgcgg cgatctgccg gggagaaacg cggacgccag tccgatacgg
    16921 cagggcggcg tccgcgcggg gaaccgcgcc cggcgaaatc cggacgccgg cgcaagggca
    16981 gatagcgcgg tttgggcaca ctcatcgtgc cggatcggcc atccgtattc atcatttaaa
    17041 ccaaaaagag cagttgaacc ttcaactctc tttggtttca ggtttcaagg tttcatggtt
    17101 tcatccaaat cgggatcggg atcgggatcg ctatcgggat cgactgtttt accacgggac
    17161 actttcgtgg gtttcagggt ttcggggttt caaggtttca aggtttcaag gtttcatcca
    17221 aatcggggtc ggggtcgcta tcgggatcga ctgtttacca tgaactcacg ggatggcact
    17281 gctcgggttt ttatccacag atttacacag attacg
//
LOCUS       DAOSRB010000042        16955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3490_length_16955_cov_6.521243, whole genome shotgun sequence.
ACCESSION   DAOSRB010000042 DAOSRB010000000
VERSION     DAOSRB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16955
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3490_length_16955_cov_6.521243"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            525..4631
                     /locus_tag="PLZ74_03820"
     CDS             525..4631
                     /locus_tag="PLZ74_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97482.1"
                     /translation="MMAVIAAGMVALGAVKVPAPDNFPGGEASIADPALWGGVLPTIS
                     DSPYFYVGDIFRYYMDGADTFTHRVDFGGTSFSDRNWVSPYTPQILTIDIFNHAVFHT
                     TAFTSTRGIDGSRHTIKVHDGGVFDCGNFKVEKGDSTIRITDGGYLVTTNASTYIGSS
                     TAGDVLHVSFLINNATWMANGFWLGRENNFENIDIAFAATNSIIESLSGGTLFNDDSD
                     FVLGSGTRLRVRDTVTIDSGAHLTLDDAEVLSDDTYTNGAWYVGVGRAKGRDSVLTVK
                     GASVLEPTGFTVGHDSARTGRVVVAENAVVDMVLPSFSYDNTRFGLSGVGYLEIRDKG
                     WFGSTSTKNGGIPIRFGINAGGYGQCDIYGGTLEYYGYGTHIGVAGTGVMNVHGGLLR
                     ILRMELGTSATETEKAVFRQTGGTVRIMSAAGKIGSEDGRVQVACVAHPAEVYLEGGV
                     LECGRLFSVNPSYATVVANGGTIRRVGSWDGAVPDKSLITGIGSFTIGVDGFTIDTTG
                     GDVDVKQNMTNIAGEEGALFKVGKEKLTFTDTAYDVANTIVAEGTFLLGDAASVLNTA
                     LTVTNNATFSLAGAATSVTLDAFTVTNGVLSIDPGDQFIVNGPVALQNICVTFSSLPT
                     VDQEYTFLVADGELDASTASALSNVLISNLLPEDTHIALVPVYDSGTGKTTVTIAVKS
                     DVPLENATVWNGSDQAWGTGGNWSAGLPTAATVAQFTNDTASASVTVAADAVAGALLF
                     AGQNYTLVGNSALTFPAEEGAAVMTVSSGVQTLDVPLVADATVKVALNEDTSLTIAKP
                     IDGGGLEKTGRGKLTLAADNMFLRPVSVRAGMTAATTAGALGSSLVSPNIVNLGDATL
                     EFPEEDGSALSLGQHFVINTTGANRTTIVKNERDVIFENGLTPSVGVFLKRGKGALTI
                     KVRDGDVLSNSDGYASFGSDSASIILDEDGTPTNRLCAAFTAVEGKLIFKPAVAGTHP
                     SISMNNTLVSGIKVPVPSQVQPEFVFEDVTVNAANKTFWLAHGCNADGEAKFPALRLV
                     NATLNLSTLSVGTWAYATDGGPTLALTNATIKAATLHLTRSNSTRSPVRLTGSHARLE
                     CRTTDAIAINGAMHCDVSDTFIGWSDGVSRTMRGNDQYTGDQIWTFRDGSELAVGAFT
                     NLLGNNNSRAFIFENALWNVGSNDLTLGSAITLPGKFSVTAAGTTGLVMRIDAGKTMT
                     FAEVPLTGTGGLVKQGAGTVAFDAGMLKCTGKLVAEAGVLDLSAAGGVTNAVVGGGDG
                     TILGGVFQDAVLLLDANPETWEVASTPLFADCSFAGRTTVSFTLDDLAGMPSPSPKHI
                     LVARYSGPKPDVSGWKLSGPANISGLFVAANNEIRMDVQHTGFMLYLR"
     gene            5276..6853
                     /locus_tag="PLZ74_03825"
     CDS             5276..6853
                     /locus_tag="PLZ74_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR97483.1"
                     /translation="MSVKQTKTMRCASGIKGSGRTIKAVCYAMACGVILSVCVLMSAA
                     YALEKSARAGGYAGEAVVFCSARWALLVNGGIAVLGLVFLIWCLVLHRQVVKKTIALE
                     DERRRAIDAQQDSEFHFQQLMDGMPDAVFIRTDGRLAYANPVACALLGASTPVELSDQ
                     PVLGFFPQGKQAYITERIAQLDAGNGYVLAVEHTLLRLDGAPVVVEITTAPLRYKGRS
                     STLVFVRDVSARKRQEQLYESLVEDTPAIICRYKPEGELTFINSAISVFLERDKDEIV
                     GANAFDFLLPEERVLAKNILRGTSPEDPRAEYTVINLRRADGQLRQMRCLSRAIIGPD
                     GSISEFQTIGFDITDQRNLEERLSQTNKLETIGLLAGGIAHDFNNVLQTILGFSELAL
                     LETPLPDPRASDIQEIITAANRAKRLTGQLLAFSRNSPMKITRVQLNQEIERDQRMLA
                     RLLGEDITIELVLDETLPEILADTGHIEQVLLNLAVNARDAMPDGGTLILQTACVTLA
                     GNDKRLQPEAAPGDYVC"
     gene            6857..7501
                     /locus_tag="PLZ74_03830"
     CDS             6857..7501
                     /locus_tag="PLZ74_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR97484.1"
                     /translation="MSDTGCGIPPHIQAKVFDPFFTTKPKDRGTGLGLSTAYGIVRQL
                     NGWILLDSEPGRGTTFSVYFPVAQKVSTEQTEESGTVPRHVRRNARILVVEDQEKVAH
                     VAERVLASHGFRTRVARGVAEAHAALAEPGAAYDLIFCDVVLRDGNGVDLAETISAQN
                     PDMRFLFTSGYVDEKSHWKAIKQRGWKCLIKPCPASDLLAAVDEALAYDTDADG"
     gene            7614..10031
                     /locus_tag="PLZ74_03835"
     CDS             7614..10031
                     /locus_tag="PLZ74_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR97485.1"
                     /translation="MRSTDESRLLNDLCRLLLEQGGCTAVWVGIVQDDITGTVRTAAH
                     AGFQENIPAAFGLSERESDAAFEAWVAQVIRTGQMQCCDDPFRAFAGMRRTFASQQGD
                     VLSLLCFPLVSPSATIGAATLIVPEPGPHPDEILGSLQKLACDMAFGIERLRIEGARK
                     RATDAQRSSELRFWRLLEGMPDSVVIQTGGRLTYANPTACTLLGASTPDELLGRPVLD
                     FFKQDKHAYIKERIAQLDTRGDYAPAVEHTLLRLDGAPVTVEMTTAPLRYRDQVRHED
                     RDGMLVFMRNITARKLQEHLYESLVEDTPAHICRFRPDGELTFASGVVSAFLGLDKGE
                     IVGANAFDFLLPEERMLARNALMNTSAEYPRAEYTVSGRRADGQLRQVRCLSRAIFGP
                     DGSASEFQMVAFDITDQLNLEERLSQTNKLETIGMLAGGIAHDFNNMLQTILGFGELA
                     LLETPPPDPRASDVQEVIAAANRAKRLTGQLLAFSRNSPMEITRVQLNRELENDQRML
                     ARLLGENIKIVLELDETLPDILADAGHVEQVLLNLAVNARDAMPDGGTLTLQTTCVTL
                     TDDDDRLHPGAVPGDYVCWSISDTGCGIPPHIQRKIFDPFFTTKPKDRGTGLGLSTVY
                     GIVRQLNGWILVDSEPGQGTTFAVYFPVAKGVPTKQTEEQGTVSRSARRDARILVVED
                     QEKVAHVVERIFASRGFDTRVVGGVAEALAALEEPGSAYDLVFCDVALEDGNGIELAE
                     KISERNPDMRFLFTSGYVDEKSGWTAIKQRGWKCLVKPCPANELLAAVDEVLARDMAV
                     PHGGSYG"
     gene            10064..13180
                     /locus_tag="PLZ74_03840"
     CDS             10064..13180
                     /locus_tag="PLZ74_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97486.1"
                     /translation="MRRIVLLAVAALCAAHGAEPWEEAVRRSAVVFDRPNGPGCVREK
                     KIHNGKVEVQAFLTGRPEYVMPVGAGNLSAMVNFGDDTFEIHLSQADWLFPEVNPGKT
                     SASDPQLMSPGHVAVKFADLSTNRITRFEQKMDMSRGRVTLSIGTTKGEIAAEIFGDR
                     ATGALVLYVNDAREGLAAPVVTTERKMESLAVVRDRAESRKFKLAIGADETAAKLSLG
                     TDDAMLAGARDSWWREYWRRGWIRLEGDEKAKNLEKWWYVNVYAYANVGYARLPPKFN
                     GGPGIVTDDIRCWGRSMWWQNTREMIWPMPAANRLEFAKRYLEFYSDCLGNLQNEIET
                     RYPEFKPFPNVIVLPEIMDILRSPLFAQGDRPLPEVTAPYVLPADAERKSSREARLAR
                     NPSHVSHIYSSGTELLQQMIEYVRFSGDRTFLPRIAKWLRANAELYLVLLEKGEDGKY
                     HTYATHVNESWWLVDDSIVDLAAARFTLAMTVAHGERFGFPKNLIAVAKDRLDHLADF
                     PHGGVYKLEKMTVAAIAPEDTVYHPCRIALGLSKANFENNELYLVFPFAMAGVDEEGP
                     RRTRALETWKHGLGAHGGFGWLPVSVAVARMRDPKAADVVYNHGHNTCGWPYGGGRSP
                     GSPMYIGAPVEDTVYLDGSCVVQTGVQELLLQSHAEEPDSRFFEGGPIRFVPCVPKSW
                     SGAFKLRARGGFIVEGTFKDGAIKKTRVTSERGNRFVWIAPATGEKKSRETKIGEIFD
                     PFARNVLPWGQDHRIGGTLLRGKDCLYAVGGGNCRGMGQIVRVELGTDGLPTGKASNV
                     GQIIEGNPGATCAAISGKWLFVAGGEIRKRTVRFEIAPDGSLTNRTEIPDENMPQFSQ
                     AGMVASGTYLIVLGGWQSRQVYAAEVSSDGGLGPWIKQRPLPSISFCGNRVFRYGNRI
                     YVSGNSVFRSTTDRVYSAEVDENGLCTRWRRFAELPEEAVAYDFTLMPDGKSVFYLSM
                     DSLAMYTAPLDQGGEIGEWRKYSETFPEGPYTAMLGIPLNASHYLRYAILLDDPRQFL
                     SADIVELE"
     gene            13281..14894
                     /locus_tag="PLZ74_03845"
     CDS             13281..14894
                     /locus_tag="PLZ74_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97487.1"
                     /translation="MKRKTVAGMTALLAGFALASMPELEPAFSATQVEKGAAKLVWRK
                     TGEGVETTVLSEQDAGLDLAGVTLTRKTSEAPGRREVVYTVENGTKELRYASFGWRTV
                     FSIADGNDRNWFPTTDNVIDLTSQSSLWGYYTKPGNWQFALVEPWFAAYNPGHKKGFA
                     FLFDFNTLCAAFGANDMMTRGVLFDGGMLPPGKKVTVTTVVRELSGLPSLATVNDRFA
                     AGFSGPETSPSLSVLAFEDLSLDGTATQTDVNGKILGSAVVKADVKAGRVANVCVIKS
                     DKPETQSVLSADLNGIKFEFFRENGFRMASLPMVPAVWTHHRPMPVKKLEVPIAVKQT
                     AKNKALLLFGFYANFFRFPEMFPEFEFTILSATPQGIMNSPPYSTIGEYKYIFMGDVN
                     EESVRPIMTRLAAYVNSGGTLVLGGGPFAYGCGGYTGTFLEEMLPVKTRPFDCLPACV
                     SDKDGRTAVAFDTGDAKVFWIHRDEVKPGAEVLLKTVAGDPLLVKGRYGEGTVYAFLG
                     APIGDAARDAKAFWNDNPSYLETLKGLLK"
     gene            14891..16774
                     /locus_tag="PLZ74_03850"
     CDS             14891..16774
                     /locus_tag="PLZ74_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97488.1"
                     /translation="MIPLVALLAAITPLRIIPNKIVYPVNESGLMTVVLTNKASDQAT
                     STVKVEEIWGIEGASRCIHEAGISLAGHEGRTLKIPWPGSRERYGREIRVTTIVDGNP
                     TGSRSEYFNVIDEWWRVNQGCGLSTARGVVTPGLKHMLDHYGYGTNDWHDCAGQMTHV
                     AWERNMPGMGPFMGYHTMETRWQMQKSSVGGNFITTTYPDDMKWITPNGSAPRWTGKI
                     RKDTRISHDWGFHHTRFTIYFMEGPYGFELARQNPEYMLRNARGQYEGRYMDVNTDPV
                     KISVQDNPQHYPWTYVEPNFFREDAFDWAIDDLVTCVTALEEDGVYFDGRYMKKQGYD
                     AFGNNLQKVYDRDLCVARNMEKTKAKIFDANPNAYIWSNGGSAADPELVIASHPQSGL
                     LNEIQWPFLLNPSRSDHSYRGFLESLLKTRNATWFGGKYVKTPSKIMHCGYLATRWDA
                     KFILEHRESFVMARHVMSIIASVCAHPFASSVVFRPFKQMMTRYSEFFWHEDMNIMKD
                     GYKKFAADSLREIWYDDLIYIRETPNFTQYLIHLVNAPEQEFCDDTVIEEPPEADDVE
                     VSTKLFAPGGVKAWTIKPHGFLSDVYEPSCIAVTPKEVKGETVFVVPPFRYYTLLVIR
                     KYK"
BASE COUNT         3608 a         4560 c         5193 g         3594 t
ORIGIN      
        1 acaccggctg cggcaaaccc ggccgtcagc aacaccaaag cggcaatcct gattcgcata
       61 atccctttta tcatggtcac gtgctccttc ctgatgcttt tgtacaatta aaggctgtga
      121 atatctgata atacaacatg ccattcatca tggcaaatgc gagatttgcg gttcataaca
      181 tcggggggga tactcatccc tttcggtctg gatcaggctt gcggaacgtc gcgaggggca
      241 agcgtctgct tgaccggcgg cagtcatgcc gccccctcca aacttcgggt cttcgcatct
      301 tcgcttgacc cctcccccct ttttgtcgcg tagaattcca tattgactgt tttgccctcc
      361 cccacataat gtgtgccaat caatgggaat cggtggggtg ccggtctgcc ttgccttatg
      421 ggatagtcgt aatcatttta gcagtcatac tgtgattcac cacaaaaggg gaaccctcaa
      481 tgagtacaaa tctatgtgaa ggtctaaaaa ggagactggt tttcgtgatg gccgtcatcg
      541 cggcaggcat ggttgcgttg ggtgcggtca aggttcctgc gccagataat tttccgggcg
      601 gtgaggcgag catcgcggat ccggcgttgt ggggtggggt gttgccgacg atttcggact
      661 cgccctattt ctatgtcggc gacattttcc gctattacat ggacggggcg gatacgttca
      721 cccatcgtgt ggattttggc gggacctcgt tcagtgaccg gaactgggtg tctccctata
      781 caccacaaat cctgacgatc gacatcttca accatgcggt tttccacacg acggcattta
      841 catcgacgcg tgggattgac ggctcgcgcc acacgatcaa ggtacacgat ggcggtgtgt
      901 ttgattgtgg caacttcaag gtcgaaaagg gtgattccac gatcaggatc acggatggcg
      961 gctatctggt aaccaccaac gcctcgacgt acatcggttc cagcacggcc ggtgatgtgc
     1021 ttcatgtgtc ctttctcatc aataacgcga cgtggatggc aaatgggttc tggctggggc
     1081 gcgaaaacaa tttcgagaac atcgacatcg cgtttgccgc cacgaacagt atcatcgagt
     1141 cattgagtgg cgggacgctt ttcaatgacg attcggattt tgtgctcggc tccggtacgc
     1201 gtctccgggt tcgggatacg gtcaccatcg attccggcgc gcatctgaca cttgacgatg
     1261 ccgaggttct cagtgatgac acgtatacga acggcgcatg gtatgtcggg gtaggtcgtg
     1321 ccaaagggcg cgactctgtc ctgactgtaa agggggcaag cgtactcgaa cccaccgggt
     1381 tcacagttgg acacgattcc gcacgcaccg ggcgggtcgt tgtggctgaa aacgccgttg
     1441 tcgacatggt tctgccctcc ttttcctacg acaatacgcg attcggtctt tccggcgtcg
     1501 gctatctcga gatccgagac aagggctggt ttggttccac ctccacaaaa aacggaggca
     1561 ttccgatccg tttcggtatc aacgccggtg gatacggaca gtgcgatatc tacggcggca
     1621 cgctggaata ctacggctac ggcacacata tcggtgtggc cggaacggga gtcatgaacg
     1681 ttcatggcgg gttgctccgg attctccgga tggagcttgg aacatcggcg acggaaaccg
     1741 aaaaggctgt tttccggcaa accggcggca cggttcggat catgagtgct gcgggcaaga
     1801 tcggatcaga ggacgggcgc gttcaggttg cttgtgtcgc gcatccggca gaggtgtatc
     1861 ttgagggcgg tgtgctcgaa tgcgggcggc tgttttccgt caacccgtcc tatgcaacgg
     1921 tggtcgcgaa cggcggaacc atccggcgtg tgggaagttg ggacggggcc gttccggata
     1981 agtccctgat tacgggtatc gggtcgttca cgatcggtgt agacggattc accatcgaca
     2041 ccactggcgg ggatgtggat gtcaaacaga acatgaccaa tatcgccggt gaggaaggcg
     2101 cgctctttaa ggtggggaaa gagaagttga cgttcaccga caccgcttac gatgtcgcga
     2161 acacgattgt cgctgaagga accttcctgt tgggcgacgc ggcttccgtc ctcaacaccg
     2221 cgcttacggt gaccaacaac gcgacgttct cattggccgg cgcggcgacg tctgtcacgc
     2281 tcgatgcgtt tacggtcacg aacggcgtac tctcgattga tccgggtgac cagttcatcg
     2341 tcaatggccc ggttgcgctt caaaacatct gcgtgacctt ctcctcgtta cccacggtgg
     2401 atcaggagta cacctttctg gtggcggatg gggaactcga cgcatcaacc gcgagcgcgc
     2461 tcagcaatgt gctgatctca aaccttctcc ctgaagatac gcatatcgcg ttggtgcctg
     2521 tttatgactc tggaaccggg aagacgacgg tcaccatcgc tgtgaaaagt gatgttccgc
     2581 tcgagaacgc tacggtgtgg aacggttccg accaagcgtg gggaaccggt ggaaactggt
     2641 ctgcgggctt accgactgcc gcgacggtcg cgcagttcac caatgacacc gcttccgctt
     2701 ctgttaccgt cgctgccgac gcggttgccg gtgcacttct cttcgccggg cagaactata
     2761 cgcttgtcgg caactcggca ttgaccttcc ccgcagaaga gggggctgct gtgatgaccg
     2821 tgagttcggg tgtccagaca ctcgatgtgc cgcttgtggc ggacgcaacg gtgaaagtcg
     2881 ctctcaacga ggatacgtcc ctgaccatcg cgaagccgat tgacgggggg gggctcgaaa
     2941 aaacgggcag gggcaagctg acgcttgcgg cggacaacat gtttctccgt cccgtatccg
     3001 tgagggccgg gatgaccgcc gcaacgacag ccggcgcgtt agggtcttca ttggtttcgc
     3061 cgaacatcgt caatctgggc gatgcgacgc tcgaattccc cgaagaggac ggttcggcgt
     3121 tgtcgctcgg gcagcatttt gtgatcaaca cgacgggtgc taacaggacg acgatcgtca
     3181 agaatgagcg cgatgtgata ttcgagaatg ggctgacgcc ttcggtgggt gtgttcctta
     3241 aacgcggtaa gggcgcgctc acaatcaaag tcagggatgg cgatgtgctc tcgaattcag
     3301 atggatacgc cagcttcgga tcggattcgg cttcgatcat tcttgatgag gacggcactc
     3361 ccacgaaccg tctttgcgca gcgttcacgg cagtcgaagg gaagctgatt ttcaagccag
     3421 cagtcgcagg cacgcatcct tcaatttcga tgaacaatac gctcgtgagc ggcatcaagg
     3481 tgccggttcc gtcacaggtg cagccggagt tcgttttcga ggatgtgacg gtgaatgccg
     3541 caaacaagac gttctggctt gcccacggct gtaatgcgga tggcgaagcc aagtttccgg
     3601 cgcttcggct tgtgaatgcg acactgaacc tctcgacgct ctctgtcggg acttgggcgt
     3661 acgctacgga tggggggcct acgctggcct tgacgaatgc gacgatcaag gccgcgacac
     3721 tccacctcac ccgttcgaac tcgacgagat cccccgttcg gttgacggga agtcatgcac
     3781 ggctggaatg ccgcaccacg gatgcgattg cgatcaacgg cgcgatgcat tgcgatgttt
     3841 ccgacacctt catcggctgg tcggacggtg tcagccgcac gatgaggggt aacgatcaat
     3901 acaccggcga ccagatctgg acattccgcg acggatccga attggctgtc ggcgcgttca
     3961 cgaatctgct cgggaataac aacagccggg cgttcatctt tgaaaatgct ctttggaatg
     4021 tggggagcaa cgatctgacg ctcggttcgg cgataacgtt accgggcaag ttctctgtga
     4081 cggccgctgg gacgacaggg cttgtgatgc gtatcgacgc tggcaagacg atgactttcg
     4141 ccgaggtgcc gctcaccgga acgggcggct tagtgaagca gggtgccgga acggttgctt
     4201 ttgatgccgg catgttgaaa tgcaccggca aactcgtggc cgaagcgggc gtcctcgacc
     4261 tttctgcggc ggggggtgtc accaatgccg tggtaggcgg gggtgacgga acgattctgg
     4321 gaggggtatt ccaagatgcg gtcctgttgc ttgatgcaaa cccggaaacg tgggaagtgg
     4381 cttcaacacc gctgtttgcc gattgttcgt tcgcgggacg gacgacagtc tcattcacac
     4441 tcgacgactt ggcggggatg ccgtcgccga gtccgaagca tattctggtc gcgaggtatt
     4501 cgggaccgaa accggatgtt tcggggtgga aactgtctgg tcctgcgaat atcagcggtt
     4561 tgtttgtagc ggcaaacaat gagatccgca tggacgtgca acacacgggc ttcatgctgt
     4621 atttacgata ggacgatgcg cgagggcgga acattcggag gatgcgtatg aggcggataa
     4681 gtgttgttgg ccatcgccgc tttgtgcgcg gcgcacggcg cggaacccgg cctccctcgg
     4741 tgtccagtat ttgacggact gcgggcattt agactcaaaa caatcgatcc cgatagcgac
     4801 cccgatttgg atgaaaccgt gaaacacatg aaaccctgaa accagaaacc ctgaaaccct
     4861 gaaacccgat cccgatagcg atcccgaccc cgatttggat gaaatcctga aaccagaaac
     4921 cctgaaaccc gatcccgata gcgatcccga ccccgatttg gatgaaaccg tgaaacctga
     4981 aaccctgaaa ccctgaaacc agaaaccctg aaacccgata gcgatagcga ttccgattcc
     5041 gatttggatg aaaccctgaa accttgaaac cctgaaacct tgaaaccaga aaccctgaaa
     5101 cccgatcccg atagcgatcc cgattccgat ttggatgaaa ccgtgaaccc tgaaaccaaa
     5161 gaaaattgaa ggttcaactt gcttactctt ttaggttgtg tcatcaaccg attccccgtt
     5221 gcttttgttg tgcctcctgc ggtatactcg cgccgtatca cgaaaaggga cgggtatgag
     5281 cgtgaagcag acaaaaacga tgcgatgtgc cagcggcatc aagggtagcg gtcggaccat
     5341 aaaagccgtt tgctatgcga tggcatgtgg cgtaattttg tctgtttgcg tgctgatgag
     5401 cgcagcgtac gcactcgaaa agtctgcgcg tgccggaggt tacgccggtg aagccgtggt
     5461 cttctgctcg gcgcggtggg cgctgctggt aaacggcggt attgcagtct tggggctcgt
     5521 ttttctgata tggtgccttg tcttgcaccg tcaggtagta aagaaaacga tcgcactgga
     5581 ggacgaacgg cgacgcgcga ttgatgcgca gcaggacagc gaattccact tccagcagct
     5641 gatggacggg atgccggatg cggtatttat ccggacagac gggcgacttg cctatgccaa
     5701 tccggtcgcc tgtgcgttgc tgggcgcatc aacacccgtt gaactgtcgg accagccggt
     5761 gttaggtttt tttccgcagg gcaaacaagc atacattaca gaacgtatcg cgcagttaga
     5821 cgccgggaat ggctatgtgc tagcagtcga acacacgctg ctacgtctgg atggcgcgcc
     5881 ggttgtggtc gaaatcacca ctgcgccgct tcggtataag gggcgtagca gcacgcttgt
     5941 tttcgtgcgt gatgtctccg ctagaaaacg tcaggaacaa ctttacgaaa gcttggtgga
     6001 agacaccccg gcgattattt gtcggtacaa gccggaaggc gagctgactt tcatcaacag
     6061 tgccatcagt gtgtttttgg agcgggataa agatgagatc gtgggagcca acgcttttga
     6121 tttcctgttg ccggaagagc gcgtgctcgc gaaaaacatc ctgaggggca catcgccgga
     6181 agacccccgc gcggagtaca cggtgataaa tcttcgcagg gcggacgggc aactgcgcca
     6241 gatgcgctgt ctgagtcgcg cgattattgg gcccgatggc tcgatcagcg aattccaaac
     6301 gatcgggttc gacatcaccg atcaacgcaa tcttgaggaa cgcctgtcgc aaaccaataa
     6361 gctggagacg atcggcctgt tggcaggcgg tatcgcgcac gactttaaca acgtgcttca
     6421 gacgatcctg ggattcagcg agctggccct gctagaaacg ccgctgcctg atccgcgcgc
     6481 ctcggatatt caagagatca tcaccgccgc caaccgtgcc aaacggttga ccgggcaact
     6541 gttggccttc agccgcaaca gtccgatgaa aatcacgagg gttcaactca atcaagagat
     6601 tgaacgcgac cagcgcatgc tggcgcgatt gcttggagaa gacatcacga tcgaactcgt
     6661 gttggatgaa acgcttcccg agattctcgc cgatacgggg catattgagc aggtgttgtt
     6721 gaatctggcc gtcaatgcac gtgacgcgat gcccgatggc ggcactctca ttttgcagac
     6781 cgcatgtgtg acgctcgccg gcaacgataa acggctgcaa cccgaggctg cgcccggcga
     6841 ttacgtctgc tgatcgattt ctgacacggg gtgcggcatc ccgccgcata tccaggcgaa
     6901 ggtttttgat ccattcttca ccaccaagcc gaaggaccgg ggaaccggcc tggggctctc
     6961 gacggcctat ggaatcgtcc gccagttgaa cgggtggatt ctgctcgact ccgaaccggg
     7021 acgcggcacc accttttcgg tgtactttcc ggtcgcccag aaggtttcaa cggagcagac
     7081 ggaggagtcc gggacggttc cacgccatgt gcggaggaac gcgaggattc tggtggtcga
     7141 agatcaggaa aaagtggcgc acgttgcgga acgcgtcctc gcctcacatg ggttcaggac
     7201 gcgcgtggcg cgcggcgtcg cagaggcgca cgcagcgttg gcggagccgg gggcggcgta
     7261 tgacctgatc ttttgcgatg tcgtgctgag ggacggcaac ggcgtcgacc tggccgaaac
     7321 gatcagcgcg cagaaccccg atatgcgctt tctgtttacc agtggctacg tggatgagaa
     7381 gtcccattgg aaggcgatca agcagcgtgg ctggaaatgt ctcatcaagc cttgtcccgc
     7441 cagcgacctg ttggctgccg tggacgaggc gctggcatac gataccgatg ccgatgggta
     7501 atcaggttat gaacgggaag gcaacaacga tggtgcaaga tgcgaaagga acggtcgcgc
     7561 tggaacggag ctaccgtatg ctccgcatgc tctccgactg caatcggatc ttggtgcgca
     7621 gcacggacga aagcagattg ctcaatgatc tgtgccgcct cttgttggag cagggagggt
     7681 gtacggcggt ctgggtcggc attgtgcagg acgacataac gggaacggtc aggacggccg
     7741 cacatgcagg ctttcaggaa aacataccgg ctgccttcgg gctgtcggag agggaaagtg
     7801 atgcagcgtt tgaagcttgg gttgcccagg tcatccgtac ggggcagatg cagtgttgcg
     7861 acgatccgtt ccgcgcgttc gccggaatgc gccggacgtt cgcctcccag caaggcgacg
     7921 tgctttcgct gctctgcttc ccgctcgtct cgcccagtgc cacgatcgga gccgcgaccc
     7981 tcatcgtgcc ggaaccagga ccgcacccag acgagatact ggggtcgctt cagaaacttg
     8041 cctgcgacat ggcgttcggc attgaacgcc tccgtataga aggcgcacga aaacgcgcga
     8101 ctgatgcgca gcggagcagc gaactccgtt tctggcggtt gctcgaagga atgccggatt
     8161 cagtggtcat ccagacaggc gggcggctca cttacgccaa tccgaccgcc tgcacgttgc
     8221 tgggcgcatc aacacctgat gaactgttgg gccggcctgt actcgatttc tttaaacagg
     8281 acaaacatgc atacatcaag gagcgtatcg cgcagttaga caccagaggt gactatgcgc
     8341 cggcggtcga gcacacgttg ctgcgtttgg atggcgcgcc ggtgacggtc gaaatgacca
     8401 cggctccgct tcgatatcgg gatcaagttc gacatgagga tcgagacggc atgctcgttt
     8461 ttatgcgtaa catcaccgcc agaaaacttc aggaacacct ttacgaaagt ttggtagagg
     8521 acacgccagc gcatatttgc cggttcaggc cggatggcga gctgactttc gccagtggtg
     8581 ttgtcagtgc gtttttgggg ctggacaaag gggagattgt gggcgccaat gcctttgatt
     8641 ttctgctgcc ggaagagcgc atgctcgcgc ggaatgccct gatgaacaca tcggcggagt
     8701 atccccgtgc ggagtacacg gtaagtggcc gcagggcgga cggacaactg cgccaggtgc
     8761 gctgtctgag ccgcgcgatc tttgggcccg atggctcggc cagcgaattc cagatggtcg
     8821 cgtttgacat caccgaccag ctcaatcttg aggagcgttt gtcacagacc aacaagctgg
     8881 agacaatcgg tatgctggcg ggcggcatcg cgcacgattt taacaacatg ctccagacga
     8941 tcctgggctt cggcgaattg gcgctgttgg aaacgccgcc gcctgatccg cgcgcctcgg
     9001 acgttcaaga ggtcatcgcc gctgccaacc gtgccaaacg gttgaccgga caactgctgg
     9061 ccttcagccg taacagtccg atggaaatca cgagggttca actcaatcga gagcttgaaa
     9121 acgatcagcg tatgttggcg cgactgcttg gagaaaacat taaaatcgtg ctcgaactcg
     9181 atgagacgct ccccgacatc ctcgctgatg cggggcatgt cgagcaggta ctgttgaatc
     9241 tggccgtcaa tgcacgtgac gcgatgcctg atggcggtac cctcactttg cagaccacat
     9301 gcgtgacgct cacggacgat gatgacaggc tacaccccgg agctgtaccc ggagattatg
     9361 tctgttggtc gatctccgat acgggttgtg gcatcccgcc gcatatccag agaaagattt
     9421 ttgacccgtt ctttaccact aaaccgaaag accggggaac cggtctgggg ctctcgaccg
     9481 tctatgggat tgtccgccag ttgaacgggt ggattctggt tgattccgag ccgggacaag
     9541 gcaccacctt tgccgtgtac tttccggttg ccaagggagt tccaacgaag cagacggaag
     9601 agcaggggac ggtttcacgc tctgcacgga gagacgcgag gatcctggtg gtcgaagatc
     9661 aggaaaaagt ggcgcacgtt gtggaacgca tctttgcttc acgtgggttc gatacgcgcg
     9721 tggtgggtgg cgtggcggag gcgcttgcag cgctggagga gccgggatct gcgtatgatc
     9781 tggttttttg cgatgttgcg ctggaggacg gtaatgggat tgaactggcc gaaaagatca
     9841 gtgagcggaa ccccgacatg cgcttcctgt ttaccagcgg ctatgtggat gagaaatctg
     9901 gttggacggc gatcaagcag cgtggctgga agtgtctcgt taaaccttgt cccgccaacg
     9961 agctgctggc cgccgtggac gaggtgctcg cccgcgatat ggcggtgcca catggcggta
    10021 gctacggatg acgtgttggg ggcggaacat ccggaggatg cgtatgaggc ggatcgtgtt
    10081 gttggccgtc gccgctttgt gcgcggcgca cggcgcggaa ccgtgggaag aggctgtgcg
    10141 gcgcagtgcg gtcgtgtttg atcgaccgaa cggtccggga tgcgtccggg agaaaaaaat
    10201 ccacaacggc aaggtggagg tgcaagcgtt cctcactgga cgacccgaat atgtgatgcc
    10261 tgtcggtgcg ggaaatcttt ccgccatggt gaacttcggg gacgacacgt ttgaaatcca
    10321 tctctcgcag gcggactggc tttttccaga ggtgaaccct ggcaaaacgt cggcgtccga
    10381 tccgcaactc atgtcgcccg ggcacgtcgc ggtaaaattc gcggaccttt ccacgaaccg
    10441 tatcacgcgg tttgaacaga aaatggatat gagcagggga cgtgtgacgc tgtcgatcgg
    10501 gacaacgaag ggggagatcg ccgccgagat tttcggcgac cgtgcgacgg gtgcgctggt
    10561 tctctacgtc aacgacgcgc gggagggtct cgcggcaccg gttgtgacaa ccgaaaggaa
    10621 aatggagtcc cttgccgttg tgcgggacag agcggagtcc aggaagttca agctcgcgat
    10681 cggtgccgac gaaacggcgg ccaagctttc gctcgggacg gatgacgcga tgctcgccgg
    10741 tgcgcgggac tcgtggtggc gcgagtactg gcgccgcggc tggatccggc tggaaggcga
    10801 cgaaaaggcg aaaaacctgg aaaagtggtg gtatgtgaac gtatacgcct atgcgaatgt
    10861 cgggtacgcg agactgccac ccaagttcaa tggtggcccc ggcatcgtca cggacgacat
    10921 ccgctgctgg ggacggagca tgtggtggca gaacacacgc gagatgatct ggccgatgcc
    10981 tgcggcgaac cgtctcgaat tcgcgaagcg ttatctggag ttctattcgg actgtctcgg
    11041 taatctccaa aacgagatcg aaacgcgcta tccagagttc aagccgtttc cgaacgtgat
    11101 tgtcctgcct gaaataatgg acatcctccg ttcgccgctc ttcgcgcagg gggatcgtcc
    11161 gcttcctgag gtgacggcgc cgtacgtgct ccccgctgac gcggagcgca aatcctcccg
    11221 cgaggcacgt cttgcgcgta acccgtcgca tgtctcccac atctattctt ccggcacgga
    11281 actcctccag cagatgatcg agtacgtgcg attttcaggg gataggacct tcctgccgcg
    11341 catcgccaag tggcttcgcg ccaatgcgga actctatctc gttctgctcg aaaaaggaga
    11401 ggatggcaaa taccacacgt atgcgacaca cgtgaacgag tcttggtggc tcgtggatga
    11461 cagtatcgtg gaccttgccg ccgcacgttt cacgcttgcg atgacggtcg cgcatggcga
    11521 gaggttcggc ttccctaaga acctgatcgc ggtggcaaag gaccgtctcg accatttggc
    11581 cgattttccg cacggtggtg tctacaagct cgaaaagatg actgtggcgg cgatcgcgcc
    11641 cgaggatacg gtttatcatc cctgccgtat cgcactgggc ttgtccaagg cgaacttcga
    11701 gaacaacgaa ctatatcttg tttttccctt cgcgatggcg ggcgtggacg aggagggacc
    11761 ccgccgcacg cgtgcgctcg aaacgtggaa acacggttta ggcgcacacg gtgggtttgg
    11821 ctggttgccg gtctccgtgg cagtggcgcg catgcgtgat cccaaggcag ccgacgtcgt
    11881 ctacaatcac ggccacaaca cctgcggctg gccttatggc ggcggtcggt cgccgggatc
    11941 tccgatgtac atcggcgctc cggtggaaga cactgtctac ctagacggtt cctgcgtggt
    12001 gcagaccggc gtgcaggagt tgttactgca gagccacgcg gaagaacccg attcgcgttt
    12061 cttcgaagga ggaccgattc gttttgtccc atgcgtgccg aaaagctgga gcggcgcatt
    12121 caagttgcgt gcacgcggcg ggtttatcgt ggaaggcacg ttcaaggacg gggccatcaa
    12181 gaagacgcgc gtcacgagcg aacgcggtaa ccgtttcgtt tggatcgccc ctgcgacggg
    12241 agagaagaaa tcgcgagaaa cgaaaatcgg cgagatcttc gacccgttcg cacgtaacgt
    12301 gttgccgtgg ggccaggacc accggatcgg cggcacgctc ctgcgcggca aagattgcct
    12361 ctatgctgtc ggcggcggca actgtagagg catggggcag atcgtgcgtg tcgaactggg
    12421 cacagacggg ctgccgaccg gaaaagcgtc caacgtcggc cagattatcg agggcaatcc
    12481 gggtgcgacc tgtgcggcga tttccggcaa gtggcttttc gtagccggtg gcgaaatccg
    12541 gaaacgaacc gtccgttttg aaatcgctcc ggacggctca ctcacgaacc ggacggagat
    12601 tcccgatgag aacatgccgc agttcagtca ggccggtatg gtggcgagcg gaacgtatct
    12661 gatcgttcta ggcggctggc agagccgcca agtctatgcg gcggaagtga gttcggacgg
    12721 cggactcggc ccttggatca aacagcgccc cttgccctcg atttccttct gcggcaaccg
    12781 cgtgttccgc tacggcaacc gcatctatgt ttccggtaat tccgtctttc ggagcacgac
    12841 cgaccgtgtc tattccgctg aagtcgatga gaacggcctt tgcacccggt ggcgccgatt
    12901 tgccgagctg cccgaagagg ctgttgctta cgacttcacg ctgatgcctg acggcaaatc
    12961 ggtgttctat ctaagcatgg actccctcgc catgtataca gcgcccctcg atcagggagg
    13021 ggagattggc gagtggagaa aatactccga aacttttcct gaaggcccct acaccgcaat
    13081 gctcggcatt cctcttaacg cctctcatta cctgcgttat gcgatcctct tggacgatcc
    13141 gcgccagttt ctctcagccg acatcgtcga actggaatga acgaaaacaa gagagtcggg
    13201 atgtctatgg cgtctttcgg tatatcgttg gggtttgcgt ccggttgtca cggagcgacc
    13261 atgtttcgag gaggggcgat atgaagagaa aaacggttgc cggcatgacc gccctgctgg
    13321 ccggatttgc gttggcatcg atgccagaac tcgaaccggc tttttccgcc acgcaggtcg
    13381 agaagggtgc ggcgaagctc gtctggagga aaaccggaga gggcgtcgag acgaccgtgc
    13441 tttcggaaca ggacgcgggg ctcgatctcg ctggcgtgac gcttacgcgg aaaacgtccg
    13501 aagcgcccgg acgccgtgag gtcgtgtaca cggtcgaaaa cggaacaaag gaactccgct
    13561 acgcttcgtt cggttggcgc acggtttttt cgattgcgga cggcaacgac cggaactggt
    13621 tccccaccac ggataacgtg atcgatctca catcgcaaag ttccctgtgg gggtattaca
    13681 cgaagcccgg caactggcaa ttcgcgcttg tcgagccgtg gttcgccgca tacaaccccg
    13741 gccacaagaa ggggttcgca tttcttttcg acttcaacac gctttgcgcc gcgttcggcg
    13801 cgaacgacat gatgacgcgg ggcgtgctct ttgatggcgg catgctgccg cccggcaaga
    13861 aggtcaccgt aacaaccgtc gtgcgcgaac tctcgggact accgtccctt gccacggtga
    13921 atgaccggtt cgccgccgga ttctcggggc cggaaacctc tccgtcgctg agtgtcctcg
    13981 ctttcgagga tctgtctctc gacggcacgg ccacacagac ggacgtaaac ggaaaaatcc
    14041 tcggttccgc agttgtgaag gcggacgtga aagccggacg ggtcgcgaac gtctgcgtga
    14101 tcaagagcga caagcccgaa acgcagtccg tcctctccgc agacctgaac ggcataaagt
    14161 tcgagttttt ccgtgagaac ggtttcagga tggcgtctct cccgatggtg ccagccgttt
    14221 ggacgcatca ccggccgatg ccggttaaga aactcgaagt tcccatcgca gtgaaacaaa
    14281 ccgcgaagaa caaggcgctc ctcctgttcg gcttctatgc gaacttcttt cgttttccgg
    14341 agatgtttcc cgaattcgaa tttacgatcc tttccgcaac cccgcaaggc attatgaaca
    14401 gtcctccgta ttcgacgatt ggcgagtaca aatatatttt catgggcgat gtgaatgagg
    14461 aaagcgtgcg tccgatcatg acgcgtcttg cagcgtatgt caatagcggc gggacactcg
    14521 ttctgggcgg cggtcccttc gcgtacggct gcggtggata cacgggaacg tttctcgaag
    14581 aaatgctgcc cgtgaagacg cggcccttcg actgtcttcc ggcgtgtgtc tcggacaagg
    14641 atggccgtac cgcagtcgct ttcgacacgg gagatgcgaa ggtcttctgg atccatcggg
    14701 atgaggtgaa acccggagcg gaggtcctcc tcaaaaccgt tgccggcgat ccgcttcttg
    14761 tgaagggcag gtacggggaa gggacggtct acgcgttcct cggtgcgccg attggtgacg
    14821 cggcccgcga cgcgaaagcc ttttggaatg acaacccaag ctatcttgaa acgttgaagg
    14881 gactcctcaa atgatacctc tcgttgctct gctcgcggcg atcacgccgc tcaggattat
    14941 cccgaacaag attgtctatc cggttaacga gtccggattg atgactgtcg tgttgacaaa
    15001 caaggcgtcg gatcaagcca cctcgaccgt gaaagttgaa gaaatctggg ggattgaagg
    15061 tgcttcccgc tgcattcatg aggccggaat atccttggcc ggccatgagg gaaggacgct
    15121 gaaaattccc tggccgggga gccgggaaag gtacgggcgc gaaatacgcg tgacgaccat
    15181 tgtggatggg aacccgaccg ggtcgcgctc ggaatatttc aatgtcatcg acgagtggtg
    15241 gcgcgtgaac caggggtgcg ggctcagtac ggcgcgcggg gtggtcacgc cgggcctgaa
    15301 gcacatgctc gaccactacg gttacgggac aaacgattgg catgactgcg cagggcagat
    15361 gacgcatgtg gcgtgggaac gcaacatgcc cggcatgggg ccgttcatgg ggtatcacac
    15421 gatggagaca cgctggcaga tgcagaaatc gtctgtcggc ggaaacttca tcacgacaac
    15481 gtatcccgac gatatgaaat ggatcacgcc gaacggtagc gccccgcgat ggaccggaaa
    15541 gatccggaag gacacgcgca tctcgcatga ctggggattc catcataccc gtttcacaat
    15601 ctactttatg gagggtccat acggcttcga actcgcgcgt cagaacccag agtatatgtt
    15661 gcgtaacgcg cgcggacagt acgaggggcg gtatatggat gtgaacaccg atccggtgaa
    15721 gatttccgtg caggacaatc cgcagcacta tccctggacc tacgtcgagc cgaatttttt
    15781 ccgcgaagac gccttcgact gggcgattga cgacctcgtg acatgcgtca cggcacttga
    15841 agaggatggc gtctatttcg acgggcgtta catgaagaaa cagggatacg acgctttcgg
    15901 gaacaatctg cagaaagtct atgaccggga cctgtgtgtc gcgcgcaaca tggaaaagac
    15961 gaaggcgaag atcttcgacg cgaatccaaa cgcctacatc tggtccaacg gaggcagtgc
    16021 ggcggatccc gaacttgtga tcgcgagtca tcctcagagc gggcttctaa atgaaattca
    16081 gtggccgttt ctcctcaatc cgtcgcgttc ggatcattcg taccgcggtt ttctggagtc
    16141 gctgctgaaa acccgtaatg cgacatggtt cggcggcaag tacgtgaaga cgccatcgaa
    16201 aatcatgcat tgcggctatc tcgcgacccg gtgggatgcg aagttcatct tggagcaccg
    16261 agagagtttc gtgatggcgc ggcatgtgat gtccataatc gcatccgtct gcgcgcatcc
    16321 gtttgccagc agtgtcgttt tccgtccttt caagcagatg atgacgcgtt actccgagtt
    16381 cttctggcac gaggatatga acatcatgaa agacggttac aagaagtttg cagccgattc
    16441 tctgcgcgaa atttggtacg atgatctgat ctacatcaga gaaacaccaa atttcaccca
    16501 gtatctgatt catctcgtga atgctcccga acaggagttt tgcgatgata cggtcatcga
    16561 agaaccgccc gaagccgatg atgtggaagt ctcgacgaaa cttttcgccc ccggcggagt
    16621 caaagcctgg acgattaagc ctcacggttt tctgtccgac gtctacgaac cctcatgcat
    16681 cgccgtgaca ccgaaggagg tgaagggcga gaccgttttc gtggtgccgc cattcaggta
    16741 ttacacgctt ctcgtaatca ggaagtacaa gtaaacctaa aaagagcagt tgaaccttca
    16801 actctcttgg gtttcatggt ttctgggttt catggtttca aggtttcatg gtttcatggt
    16861 ttctggtttc atcaaaatcg gggtcgggat cgggatcggg atcgggatcg ctatcgggat
    16921 cgggaacgtc gacccgcgac ccgcgacatt cgacc
//
LOCUS       DAOSRB010000043        16886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3504_length_16886_cov_6.612620, whole genome shotgun sequence.
ACCESSION   DAOSRB010000043 DAOSRB010000000
VERSION     DAOSRB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16886
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3504_length_16886_cov_6.612620"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..195)
                     /locus_tag="PLZ74_03855"
     tRNA            complement(119..195)
                     /locus_tag="PLZ74_03855"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Met,seq:cat)
     gene            complement(212..288)
                     /locus_tag="PLZ74_03860"
     tRNA            complement(212..288)
                     /locus_tag="PLZ74_03860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(252..254),aa:Arg,seq:acg)
     gene            664..2394
                     /locus_tag="PLZ74_03865"
     CDS             664..2394
                     /locus_tag="PLZ74_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase N-terminal
                     domain-containing protein"
                     /protein_id="HOR97489.1"
                     /translation="MVRRGRRGIEFIGICVAIVFAWGMAQAAEGGVGGRVVLDANRNG
                     RAEPGEPGVAGVAVSDGVQVVVTDASGAYRLARRPEATTPIWICVPRDHAVSGSFWKA
                     AGTEGDVDFGLTPQAQDEAFTFIHFTDTHIGRGDLLKRFAEQTGTYPMRIAFAVNTGD
                     LVGGVDFVPPEKAPAQYERYMEAARFPMPLFNLPGNHEHVGINYKGADREHPFYGKGL
                     YPRLLGPMHYSWDWAGVHCVALDGTSLPYQERLGSNQLAWLEADLALQPAEKPVLLFC
                     HQSIPSLRDATLLAECLKGRNVLGAFCGHLHRTFTATFAGFPVYHSGALSGAWWGGPN
                     IDGTPQGFRLVQFRDGKVKTVYTNWEGAYPVSIVEPLADTVQTGAVKVTVSVTDFGRP
                     AEIAANYCGQPVALRLDSREELWSFWSGTLDTRTAFDGDCTLTVTAICEQVSNTFAIR
                     YLVENGRSGTYAAAEPARLCLQVRGGIDAPGEVQIHGEPLGSIPQGTTNETILAFAIP
                     PERLRKLNRVTVLAARQGQGWDRFNIGPVWIEYQGKRIYDLRYASFERHGVGGTHPAD
                     REKVLYYCLP"
     gene            complement(2410..8280)
                     /locus_tag="PLZ74_03870"
     CDS             complement(2410..8280)
                     /locus_tag="PLZ74_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOR97490.1"
                     /translation="MKRNAQIVLILSACACLQVMVTSAQDIRAAGELFVHLDATNVTG
                     LSDGAYVATWPNHGTLKDFVPAFPGQGATYVSQLAGAAALQFDGTVTSTMVQNGHTNN
                     ASAGGVPLSILGTNVWSAELWFNNAAGGAIETLLTWTSRRSAGNRQLMEIRHYNTTDA
                     VEHYARNMTWSSQAPCFGQWQHLAVTRDALGMQRLYVNARLVSTVDMGGSNMLNLKPN
                     HALFAIGSVDAWGGWEKPLSAAVAVVRVHDGTLSPEDVLHNFMVERSRFGGGWNNPGT
                     GDWNEPANWENGAIPYQDAPLYINNGGTATYDGTPYENATQTAMLYGIEGGLTVADGI
                     FHALPLIMNDGLLRLGIAPGKTFTLGAVSGGSFTTLGKSLMLGDIAGATGSVSLASAG
                     RLTVARIQKGAGTALLTVDGGVLEATTSANNFLQGLTRAKVKANGICFDVPDQTAITV
                     PQNLLTDPDSPGGGLTKTGPGTLSLTGVNTLTGALQVTGGELVIQNNAFATNYASPIT
                     LANDGVIGWNQDGGAASIASNLTAGSVGTLKLYVPNAAENIDFSALPGMQLVTSGSFN
                     YTGVFTPYANRFVFRPDKGVMVFPQTIANLPDLPGCVEVRGTASGYVDLTADNSYTGG
                     TEIVSGGVIVSHANALGLGNDGVRDILCASGTVMRVQTLLSDPSFFSRVQAEPYAALQ
                     LSGAGLNQDIDLSDCPNLFTGSENITSRSYFNGVLTPYGNTYLLGNSGVDAATYYMGN
                     GFVITNLTDGPGGEPRAVLVSGIGAVDTRNQAAYSGGTRIENGARLIVTGDGGLGAVP
                     ATDDPDNIVIDGGILRTANPFISFAPTRGFTIGPKGAVFHAPGPLPAQLIIPGNLTGS
                     GTMRMTDMGWVTFTGTNNSYQGRVQLEGMVGAMMIGDGTNFSWTSTGGIVGTGTYGWL
                     YLNNSGENTFGDAFSGKGLLTKKGTGTITLTAAQSNRKLPVNTVIESGALRYGLANAL
                     SFGTADAGAVQINTGATLDLNGFSSALNGLAGTGCVTNRAGTAVTLQVGATDAASQFD
                     GQFAPGVTLEKIGTNTLLLTHAEPSRQNVSVAAGTLALYPGVALTNGITLTSDAAVRA
                     ASHRGLRAEYYDAVMPQGVPAIWPALGTTVEAVEELLSGRTPSLVTDCASFGDFFDSG
                     ELGTAFPGKYNQHSENYFVARYTGVFTAEEAGTYEFSLFADDGCMLFLDNTLVVNNRT
                     GKGTATGSITLEQRDYAILLFFYESAYSATLQLQMRGPSDAALSMLPQRLLKPASPAV
                     AVIGGILAAEGGRFEADSFGHLVLTDEGDYSAAGFGGPGITNAVIEKQGAGALTLTGT
                     SSFRGTALLSEGDIILANGTSHSNAFDVLNGTLTLSGGTSIDDAPVWIGSLNVAASGE
                     LALASKTFLRIYQDEAGIFAGTLSGGTADSVIAKDGPAELVLASDCSAYPGFWQVLAG
                     DLVITDNDALAADTVVTLERDGCLVFRCDGDVSFAGTVTGPGTIRNDGPGTLTLTGLT
                     TSGIEVPAGQTVILDGGEGDTSRELESDIVNNGTLRIAYPGGVLLGNPVSGTGTVSVA
                     SNTVVTVLRAGLLADDASLRLDGGTLTLHSGGTLGFNETTWQTNGVSRFVTNETGDVI
                     LEANPNVKSTVGSAFHKLRIPATNAWELNAVWRKGAGGGCGGGDGFAIMLQNDARGAS
                     ALGSSGSIGISTITPSFGYRFYLLSQKFAWVENGSAVGEFVTNLFSWGGGVFTADMAF
                     DGNQLTVTMSQGTKTLTVTNTNARAKLAAVGADAYVGLGSATGGCWGQQFIDSFSFSS
                     LWEPGYLFTNDLTLAANSASTIQAAARAVEGLPLEIGDLIFENGSALTAVPSADSDPD
                     LVFARFGNLTVRGASACHIDDAGWAAFRGTTWTFVPGAVLTVTGTVTLPETVLVAIEG
                     EIPQGRTDLANFRGADIMNAESVNFVLENDDGKTFLIFRDGILYVSRIQGSVLFLR"
     gene            8940..9995
                     /locus_tag="PLZ74_03875"
     CDS             8940..9995
                     /locus_tag="PLZ74_03875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR97491.1"
                     /translation="MSSGKQMLWWGRFDPAYSRNGVVRKHLTALGWTLRDFHPAVCAW
                     GDAEAALRRIPAPDCVWVPCFRQRDLAAARRWCDRRGVPLVFDPLISAYDKQVWERFK
                     YKDGSPAARKLLLWERGLFARADLVIADTVCHAAFFTEVLGVREEKIRVVCVGADETL
                     FRPAPERASGSPLDVLFYGSFIPLHGAQTIIEAARLDPGLPVRWSLLGDGPARPACQK
                     AAEGLTNVFFEDRIPYAQLPARIHRADILLGVFGTTRKAGRVIPNKVFQALASGRPVI
                     TRTSEAYPAAVRESEALSFVAPGDPQALADAVAAWAAQREHLHVRGQAAYKVYQSAFS
                     ADVVARQLRNVFASLSL"
     gene            complement(9987..11072)
                     /locus_tag="PLZ74_03880"
     CDS             complement(9987..11072)
                     /locus_tag="PLZ74_03880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOR97492.1"
                     /translation="MKIGIVTLCEANNYGGVLQAYALQTYLRRMGHESAVLPVDYAKR
                     RNSPLKVLERPVKTVMEAVKAKKFASFRAKHFRSPGVTACRVDAFLRQPPRFDAYVCG
                     SDQVWSRRACADSELRKYFFLDFGEVTARRISYAASWGAQTLEPAVRDDIRACLQKFT
                     ALSVREKSGQGIVADLGFTAQWLPDPTFLLEDEEWRRLAEDEPCTLGDNVLFQCEYRW
                     TPCVRLSEINRVLVRQFGWDTVIPFSDHPFRDAVCTRCLTPSQWVNGIRRASLVLTNS
                     FHGMVFSIIFRRPFIAVPLAGKYAGMNERFLSLAERLGLQDRLLTDPDPSRIIALAQA
                     PIDWESVYRHLNDWRREASTFLASALQ"
     gene            complement(11101..12285)
                     /locus_tag="PLZ74_03885"
     CDS             complement(11101..12285)
                     /locus_tag="PLZ74_03885"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOR97493.1"
                     /translation="MNLNPVHGDENTQPLLLCATEDCTGCSACSAICPESCVALLPDR
                     EGFLRPHVDSARCLRCGLCTNICPGLHPGLPAREPVAVYAAQNTCAAVREESSSGGVF
                     SLLAERVLERGGVVFGAGCDATFRVVHQEVEDAADLARLRGSKYVQSDMNDIFRRVRQ
                     HLGAGRQVLFSGSPCQVAGLRAFLGKAFDRLLCVDFVCHGVPSPRVFELWKRRLEKRS
                     RSAITGISFRNKACGWRQFSFAATFADGRTYRETVKEELYLRGFLSNLFLRPACHACR
                     FKEMKSGSDLTLADYWGVEKRFPCFRDNRGTSLVLANSAKGVEAFRSCASGVEFRDSD
                     YAHAVACNPSIVRSSRPHPDRARFFARLETHELGGLLLRMAKLSFRRRVKLFVKHALG
                     MI"
     gene            complement(12533..16366)
                     /locus_tag="PLZ74_03890"
     CDS             complement(12533..16366)
                     /locus_tag="PLZ74_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97494.1"
                     /translation="MIMCLAVAICGAASITFASTSEVGTYNWINTTAKTPGWGTDANW
                     ENGDWPLIKDTPAMTNSTFVFNGDLAIRQEIKDLEASYDVPAWLGTVLGSASHVLNCV
                     NSFSHRYTTIRDASSFEGSLGVAAGIGGTWLLLSDNAWTTTVANARMISNPAFLVRTA
                     GAKAVIERAWGRGRYLVNFANKTTHGTGAAEIEVIDSQGGPGADPHVVDGTFTVNGRS
                     YEAPALAGDPWMHLDASSSASLTLTLQDGTNYVTSWADVRANGLAAIQDGAAQCPRLE
                     TDAVSGLPIIDFGGFSPLTESVKAYGPAGTLIFNAGERSDIREVFVVFRDAPTKKPVP
                     SNTGIVCLQNFLCRAVGEGQYFTRRFSKRVNQTYYYGGLFDISVTTSGTKLTQPLASA
                     DVRLNGVRIEPEDVDDYSRHLNVLSVGLAHPVAADRFASTVDGHTGGIAFGEVLIYTN
                     ALTSAERRRVNAYLRAKWQPAEVAADLDFGRVTLNSGAQLKVNSGSVLVDEVNLNDTT
                     FVKQGAGGLRVKKLTGQTLPKVNVTGGRLDFADTCVTASFTAPDEPPVAPETWFDANC
                     GVSVFTTNVFDTEMEYVSQWVDRRDGTNLYGHTVVARRINTAGNYVNVGDSCFPTIRK
                     AYAAANGRDMMDFGPQRTNLTLNKTNFWVWAEPKDASAMYIRIEDAAPWYYNHEGYLV
                     ISPNTNRVITVGTVDFMPGDKGRLLGYDAYGHVQGAVWTVNGERVDPLSFVLEPGQVY
                     VIGYSLPVFGPAYGAGGSGVQNFAFLPPGYGSGTWGGIAIGEALTYPHKLTPLERRAT
                     EAYLLKKWLNREHPDAVHAQEGRALASIDYAEGATPELDVEGSLRVGVINGTGISFVK
                     SGSGSLDVGTLPHDVASIRVEGGSLSTGIDGMSDAFLHVDASDASSFEFRTEPGMEDQ
                     IVKWKDTRGNGMYAALPSEDRGLTNAVYRTATYPGLVEGLPCVDFLDSVEGAKPAGTT
                     LYVATVTNTSSGMCWYNAAGSQVTCPNLRECYIVFCDHEGESTGSFIGAKNSTFGARA
                     PNYTTIFPWYSQILTWIDQNTVTTNKYNFSENPGFHVLTIVTTNAADMVEGAYFCIDR
                     NGLRYGGCRIAEAVIFDKLLDDGRRQGIHNYLLKKWRAIGEGAVMTADLESVSVADGA
                     SLALESESRIRTAVLPGGDVTVSAPMEVTATAQSPWNYKVAFSAADSWNRLTVNGDLS
                     LPDYAAVTLTAPASLKLPTGLYPFITATQLTADISRWTLDNSDAPGLVTYKLVKTGNA
                     IAIHVSPKGVVFSIR"
BASE COUNT         3231 a         4988 c         5343 g         3324 t
ORIGIN      
        1 gtaaatgacc cctataaatc agcttgtttc agattgtagc agggcaaagg gcaactatgc
       61 cgcacaaaca aaaaaccccg cttttattgg gctttttcaa taaaaccgag gctgaaattg
      121 gtagcggggg taggatttga acctacggcc ttcaggttat gagcctgacg agctaccagg
      181 ctgctccacc ccgcaatcag gttgtttttc ctggcgcgcc tcgcaggagt tgaacctgca
      241 gccttctgat ccgtagtcag atgctctatc caattgagct agaggcgctc ttgaaacggt
      301 atacactgta acaaaatccg ggcacgggcc gcaaggtgta ttttggccag atgcgatttt
      361 cctcacttgt caaagtaaat gcccccgcct tggccccgct ctgggggcat ttgctttgac
      421 aagccacatt tgaaagagaa tgggtttttt tcacggtaaa gcctttgtgt ccgttgtgtg
      481 agacgaaatt gggttgcggg ctccgtccgc attggtatag gagagcggtg cgggtggatc
      541 agggtcaatt ccgggctggc cggcactgtg gggaatttgg tatgagtgga aagaagaccg
      601 gttgatccgt tacggcgcgc ggagaccggg gggcaatcag gtagcgcagc taaggagggg
      661 tatatggtac ggagaggcag gcgggggata gagtttatcg ggatatgtgt cgcgattgtt
      721 ttcgcatggg gtatggcaca ggccgcagag ggcggcgtgg ggggcagggt ggttctcgat
      781 gccaaccgta acgggcgcgc cgaacccggc gagcccgggg tggcgggggt ggcggtgagc
      841 gacggtgtgc aggtagtggt gacggatgcc tcaggcgcgt accggctggc gcggcggccg
      901 gaggcgacga ccccgatctg gatctgcgtg ccgcgggatc acgcggtgag cggttcgttt
      961 tggaaggcgg ccggcacgga aggagacgtg gatttcggtc tcactccgca ggctcaggac
     1021 gaggcgttca cgttcatcca ctttacggat acccatatcg gacggggtga cctgttgaag
     1081 cgtttcgcgg aacagaccgg tacctatccg atgcggatcg cgtttgcggt caacaccggg
     1141 gatctggtgg ggggtgtgga ttttgtgccg ccggaaaagg ccccggcgca gtatgaacgg
     1201 tacatggagg cggcccgctt cccaatgccg cttttcaacc tgcccggcaa ccacgagcat
     1261 gtcgggatca attataaggg cgctgaccgg gaacatccct tttacggtaa ggggctctat
     1321 ccgcgtctgc tcgggccgat gcactattcg tgggattggg ccggcgtgca ctgtgtggcg
     1381 ctggacggca cctcgctgcc gtatcaggag cggttgggaa gcaatcaact ggcttggctg
     1441 gaggcggatt tggcgcttca gcccgcggaa aaaccggtat tgctgttctg ccaccagtcg
     1501 atcccttccc tgcgggatgc aacgctgctg gcggaatgcc tcaaggggcg caatgtgctc
     1561 ggtgcgtttt gcggccatct ccaccggacc ttcaccgcga cgtttgcggg ttttccggtg
     1621 tatcacagcg gagcgttgag cggcgcatgg tggggaggtc cgaacatcga cgggacgccg
     1681 caggggtttc ggctggtaca gttccgggac ggcaaggtga agacggtcta cacgaattgg
     1741 gagggcgcgt atccggtttc cattgtggag ccgctggccg acacggtgca gaccggagcg
     1801 gttaaggtga cggtgtcggt gacggatttc gggcggccgg ccgagatcgc tgcgaattac
     1861 tgtggtcagc cggtagcgct ccggctcgac tcgcgcgagg aactctggtc tttctggagt
     1921 ggcacgctgg acacgcgcac ggcattcgac ggcgactgca cgctgaccgt cactgccatc
     1981 tgcgagcagg tttcgaacac gtttgcgatc cggtatctgg tagagaacgg gcgttccggg
     2041 acctatgcgg ccgcagaacc ggcccggctc tgtctacagg tacggggcgg gatcgatgcg
     2101 ccgggcgagg tccagattca cggcgaaccg ctgggaagca ttccacaggg tacgacgaac
     2161 gagacgatcc tcgcgttcgc gatccccccg gaacgcctgc ggaagctcaa ccgggtgacc
     2221 gtgctcgcgg cgcggcaggg gcaggggtgg gaccggttca acatcggccc ggtgtggatc
     2281 gaatatcagg gcaagcggat ttacgatctg cgctatgcct ctttcgaacg gcacggggtg
     2341 ggcggaaccc accccgcgga ccgggaaaag gtcttgtact actgtttgcc gtaaaaccgc
     2401 agggcaggct caacgcagga acaacacgga accctgaatc cggctgacgt agaggatgcc
     2461 gtcgcggaaa atcaggaagg tcttgccgtc gtcgttctcc aggacaaagt tgacggactc
     2521 cgcattcatg atgtccgctc cgcggaagtt tgccagatcg gttctcccct gcgggatctc
     2581 gccttcgatg gcgaccagca cggtctcggg caatgtgacg gtgccggtca ccgtcagcac
     2641 ggcgccggga acaaaggtcc aggttgtgcc gcggaaagcg gcccatcccg catcgtcgat
     2701 gtggcaggcg gaggcgcccc gtaccgtgag gttgccaaaa cgggcgaaaa ccaaatccgg
     2761 gtccgagtcc gcggatggga ccgcagtcag ggcggagccg ttttcgaaga tcagatcgcc
     2821 gatttccagc ggcaggcctt cgacggcccg agcggccgct tggatcgtcg aggcgctgtt
     2881 cgccgccagg gtgaggtcgt tggtaaacag gtagccaggc tcccagagcg acgagaacga
     2941 gaaggagtcg atgaactgct gtccccagca accgccggtt gcactgccga gcccgacgta
     3001 ggcgtctgcg cccaccgcag ccagtttcgc acgcgcgttg gtgtttgtca ccgtgagtgt
     3061 ttttgtgcct tggctcatag tcacggtgag ctggttaccg tcgaacgcca tgtcggcggt
     3121 aaagacgccg cccccccaag agaacaggtt ggtcacaaac tcgcccaccg cagaaccgtt
     3181 ctcaacccag gcaaattttt ggctgagcaa atagaatcga tagccgaagc tgggcgtgat
     3241 ggtcgagatg ccgatagagc cggaagaacc cagtgcgctt gctccgcgcg catcattttg
     3301 cagcatgatc gcgaacccgt cgcctccgcc acaacctcct cctgcgcctt ttcgccaaac
     3361 ggcgttgagt tcccaggcat tggtggcggg gatgcggagt ttgtggaacg ccgatccgac
     3421 agtgcttttg acattggggt tggcttccag aatcacgtcc ccggtctcgt tggtcacgaa
     3481 ccgtgaaacg ccgttggtct gccatgtggt ctcgttgaaa ccgagcgtcc cgccgctgtg
     3541 gagcgtcaac gtgccgccgt ccagccgcag ggaggcgtcg tcggcgagga gcccggcgcg
     3601 gagaacggtc accacggtgt tgctggccac cgacacggtc ccggttcccg agaccgggtt
     3661 tccgagcagg acgcctccgg gatacgcgat acgcagcgta ccattattga cgatgtcgct
     3721 ttccagttcg cgggaggtgt cgccctcccc tccgtccaag atcacggtct ggcctgcggg
     3781 aacttcgatg ccgctggtgg tcaggccggt cagcgtgagc gtgccgggtc cgtcattgcg
     3841 gattgtgccg ggaccggtca cggttcccgc gaaggacacg tcgccgtcgc aacggaagac
     3901 cagacacccg tcacgttcca gagtcaccac ggtatcggcg gcgagcgcgt cgttgtcagt
     3961 aatcaccaga tcgcctgcca agacctgcca gaaaccggga taggcgctgc aatcactcgc
     4021 gagcaccagt tcagccgggc cgtccttggc gatcacactg tcggccgttc cgccgctcag
     4081 ggtccccgcg aagattccgg cctcgtcctg atagatgcgc aggaaggttt tcgatgccaa
     4141 cgcgagttca ccggaggcgg cgacattgag cgagccgatc caaactggcg cgtcgtcaat
     4201 cgacgtgccg ccgctgaggg tcagtgtgcc gttcagcacg tcaaacgcat tggagtgcga
     4261 cgtcccgttc gccagaatga tgtcgccttc tgaaagaagc gcggtgccgc ggaaagagct
     4321 ggtgccggtg agcgtgagcg cacccgcgcc ctgtttttcg atcaccgcgt tggtgattcc
     4381 cggtccgccg aaacccgcgg ccgaatagtc tccttcatcg gtcagcacca gatgaccgaa
     4441 ggagtcggcc tcgaaacgcc ctccctctgc ggcgagaatg cctccgatca ctgcgactgc
     4501 ggggctggcc ggtttcagca accgttgcgg caacatcgag agcgccgcat ccgagggccc
     4561 ccgcatctgg agttggagcg tagcggagta ggcgctttca taaaagaaaa gcaggatggc
     4621 gtaatcgcgc tgctccaagg tgatgctgcc ggttgcggtt cccttgccgg tgcggttgtt
     4681 gaccaccagt gtgttgtcga ggaacagcat gcagccatcg tccgcgaaga gactgaactc
     4741 ataggtcccc gcctcttcgg ctgtgaacac tccggtgtag cgggcgacga agtaattctc
     4801 actgtgctgg ttatattttc cggggaaggc ggtgcctagt tcgccggagt cgaaaaaatc
     4861 gccgaagctg gcgcaatccg ttaccaggct cggagtccgg ccgctgagaa gctcctcgac
     4921 cgcctcgacc gtggttccca gtgcgggcca aatggccggg acgccttgcg gcatgacggc
     4981 gtcgtagtat tctgcgcgga gaccccggtg cgaggcggcc cggaccgcgg cgtccgaagt
     5041 caacgtgatg ccattggtga gcgcgacgcc ggggtagagc gccagggtgc ccgccgcgac
     5101 cgacacgttc tggcgtgagg gttcggcgtg ggtcagcagc agcgtgttgg tgccgatctt
     5161 ttccagcgtg acgccgggcg cgaactggcc gtcgaactgt gaggccgcat ccgtagcccc
     5221 gacctgtagg gttactgccg taccggcgcg gttggtcaca cagcccgtgc cagcgagacc
     5281 gttgagtgcg gaggagaagc cgttgaggtc gagtgtcgcg ccggtgttga tctgcacggc
     5341 acctgcgtcg gcggttccaa aggagagcgc gttggccaaa ccgtaacgca gtgcgccgga
     5401 ttcgatcacg gtgttgaccg gcagtttccg gttgctctgc gcggcggtca gcgtgatggt
     5461 tcccgtgcct tttttcgtca ggaggccttt acccgaaaag gcgtcgccga aggtattctc
     5521 tccgctgttg ttcagataga gccagccgta cgttcccgtg cccacgatgc cgccggtgga
     5581 ggtccacgag aagttcgtgc catcaccgat catcatcgcg cccaccattc cttcaagctg
     5641 cacacgccct tggtaactgt tgttggtgcc ggtgaacgtg acccagccca tgtccgtcat
     5701 gcgcattgtg ccggagccgg tgagattccc cgggattatg agctgcgcgg gcagcggacc
     5761 gggggcgtgg aagaccgcac cttttgggcc gatcgtgaac ccgcgtgtcg gggcgaacga
     5821 gatgaacggg ttcgcggtac ggagaatccc gccatcgatc acgatgttgt cgggatcgtc
     5881 ggttgcggga acggcaccga gtccgccatc accggtgacg atcaggcggg cgccgttctc
     5941 aatgcgtgtg ccgccggaat atgcggcctg gttgcgggta tccacggccc cgattccgct
     6001 gacgaggacg gcgcggggct cgccacccgg cccgtcggtc aggttggtga tgacgaatcc
     6061 gttgcccatg taatacgtgg ctgcgtctac gccgctgttt ccaagaagat aggtgttccc
     6121 gtatggagtg agcacgccgt tgaaataact tctggacgtg atgttctccg aaccggtgaa
     6181 caggttcgga cagtcagaca ggtcgatatc ctgattcagt ccggcaccgc tgagttgaag
     6241 cgcggcatag ggttcagcct gcacgcggct gaagaaggac gggtcggaca gcagggtttg
     6301 gacccgcatc acggtgccgc tggcgcagag gatgtcgcgc acgccgtcgt ttccgaggcc
     6361 cagcgcattc gcgtgcgaga cgatgacccc gcccgaaacg atttccgtgc cgccggtgta
     6421 actgttgtcg gccgtgaggt ccacatatcc gctggcagtg ccgcggactt cgacacaccc
     6481 cggcagatcc ggcaggttgg cgatggtctg cgggaaaacc atgacaccct tgtcgggtct
     6541 gaaaacgaac cggttcgcat agggggtgaa gacgcctgtg taattgaagg agccggaagt
     6601 caccaactgc atgccgggca gggcggagaa atcgatgttt tccgccgcgt tcggcacata
     6661 gagtttcagg gtgccgacgg acccggccgt gaggttcgag gcgatcgagg ctgcgcctcc
     6721 gtcctggttc cagccgatga ccccgtcgtt ggcgagggtg atcggcgagg cgtaattggt
     6781 ggcgaacgca ttattttgga taacgagttc cccgccggtc acctgaaggg cgccggtaag
     6841 cgtgttgacg ccggttagcg agagggtgcc ggggccggtt ttggtcagac cgccgcccgg
     6901 gctgtcgggg tcggtcaaca ggttttgagg gacggtgatc gcggtctggt cgggcacatc
     6961 gaaacagatc ccgttcgctt tcactttggc acgcgtgagc ccctgcaaga aattattggc
     7021 agaggtggtg gcctccagca caccgccgtc cacggtcagc agggcggttc ccgcgccttt
     7081 ctggatacgc gcaacggtca ggcgccctgc cgaagcgagc gagacggagc cggtcgctcc
     7141 agcgatgtcg ccgagcatca gcgatttgcc gagggtggtg aacgatccgc ccgagacggc
     7201 cccgagggtg aaggttttgc ccggggcgat gcccagacgc agcaagccgt cattcatgat
     7261 cagcggtagg gcgtggaaga tcccgtccgc gacggtcagg ccgccttcga ttccgtacaa
     7321 cattgcggtc tgggtggcat tctcataggg agtgccgtca taggtggcgg tgcccccgtt
     7381 gttgatatag agcggcgcgt cctgataagg gatggccccg ttttcccaat tcgcaggctc
     7441 attccagtcg cccgtgcccg gattgttcca gccgccgccg aagcggctgc gttcaaccat
     7501 gaagttgtgg aggacatcct cgggactcaa tgtgccgtcg tgcacacgca ccacggcgac
     7561 ggcggcactc agcggctttt cccatcctcc ccatgcatcg accgaaccga tggcgaaaag
     7621 ggcgtggttg ggcttgaggt tcagcatatt gctgccgccc atatccaccg tgctgaccag
     7681 acgggcattg acgtacagcc gttgcatgcc gagcgcatcg cgcgtgacgg cgagatgctg
     7741 ccactggccg aaacagggtg cctggctgct ccatgtcatg ttacgcgcat agtgctcgac
     7801 cgcatctgtc gtgttgtaat ggcggatctc catcagttga cgattgccgg cgctgcgacg
     7861 cgaggtccag gtcaggagcg tttcaatcgc acctcccgcg gcattgttga accacagttc
     7921 agccgaccag acattcgtgc ccagaatact cagcggcaca ccgcctgccg aggcgttgtt
     7981 ggtgtgaccg ttctgcacca tggtgctggt gaccgtgcca tcgaactgca aggccgctgc
     8041 cccggccaac tgggacacgt acgtggcacc ctggccgggg aaggcgggca cgaaatcctt
     8101 cagggtcccg tggttgggcc aggtcgcgac ataggcgccg tcggaaaggc cagtcacgtt
     8161 ggtcgcatca agatggacga agagttcgcc cgcggcacgg atatcctgtg ctgaggttac
     8221 catgacctgc aagcaggcgc aggccgacag aatcagaacg atttgcgcgt ttcgtttcat
     8281 accactcctt tgcccttttt ggaaatgtag gattattatg aactcaacaa ttcgatccgt
     8341 caatcacgca ctctgggaga gagaggtaat cggttcgtga cgggggggaa gagtcatcgt
     8401 gtacgtgtac gtgaacgagt acgtgtacgg ggtatagcca acgttcacgt tcttcaaatc
     8461 gctatcgggt tcggtatcgc tatcgggttc gctatcgggt tcgaaatcga ctgttgcgcc
     8521 cgcgcagtgg cgcgcagtga caagtgtaat gatggcggcg attccgatcc cgatggcgat
     8581 agcgatggcg ccgcaatgga accctggaac gcatgacctt cgatcgtacc cctgtcacga
     8641 accgattacg ggagagagag gacgggcgca tcctctttag gccgaattgc gttgagggga
     8701 gcgggcttcg atgatatgct tttgacattc gctgtttcgt gcgcggtaag gcggaaaagg
     8761 agattcatgc gtcacgcaaa gcacgttctt tcaatcgcct tcggcagacg gtgtttcgag
     8821 gcatatcaga agatccgaaa ggagtgtggc gaacgctgaa tgccgaacgc ggaacgcgga
     8881 gggttgtccc cggatgactt gcgcgttagg cgatcggacg ggaagcgttg ggcgttctca
     8941 tgtcttccgg aaaacagatg ctctggtggg gccgttttga tccggcctat tcgcgcaacg
     9001 gtgtggtgcg caagcacctg accgcgctgg gctggaccct gcgcgacttt catccggctg
     9061 tctgcgcgtg gggcgatgcc gaagccgcgc tacgccggat tcccgcgccg gattgcgtgt
     9121 gggtgccctg tttcaggcag cgcgatctgg ctgcggctcg ccgttggtgt gaccgccgcg
     9181 gtgtgccgct cgtgttcgac cccctcatca gtgcctacga caaacaggtg tgggaacgct
     9241 tcaagtacaa agacgggagc cctgcggcgc ggaaactctt gctttgggag cgcggacttt
     9301 tcgcccgcgc cgatctggtg attgccgaca ccgtctgcca cgccgctttc tttacggagg
     9361 ttctgggtgt gcgggaagaa aagatccgcg tcgtctgcgt cggcgcggac gagacgcttt
     9421 tccggcccgc gccggagcgt gcgtccggct caccgctcga cgtgcttttc tacggaagct
     9481 tcattccgtt gcacggtgcg caaacgatca tcgaagccgc gcggctcgat ccggggcttc
     9541 ccgtgcggtg gagcctgctc ggcgatggcc ccgcgcggcc ggcctgccaa aaggcggcag
     9601 aggggctgac gaacgttttc tttgaagacc gcatccctta tgctcaactg ccggctcgta
     9661 ttcaccgcgc cgatatcctg ctcggtgtgt tcggcacgac gcgcaaagcc ggacgggtca
     9721 tcccgaacaa agtgtttcag gcgctggcct cgggacgtcc ggtcatcacg cgtacgtcgg
     9781 aagcgtaccc ggcagcggtc cgcgaatcgg aggcgctgtc ctttgtcgcg cctggcgacc
     9841 cgcaggcact ggctgacgcg gtggccgcct gggccgcaca gcgtgaacac ctgcatgtcc
     9901 gaggccaagc ggcttacaag gtgtaccagt cggcgttttc cgctgatgtc gttgcccggc
     9961 aactgcggaa cgtgttcgcc tcactctcac tgtaaggcgg aggcgagaaa ggtggaggct
    10021 tccctccgcc aatcattcag atgccggtac accgactccc agtcgatcgg cgcttgggca
    10081 agtgcgataa tccggcttgg gtccggatcg gtcaacagcc ggtcttgcag tcccagccgc
    10141 tctgccagcg agagaaaccg ttcattcatg cccgcgtatt ttccggcgag cggcactgcg
    10201 ataaatgggc gccggaaaat aatgctgaac accatcccat gaaacgagtt ggtcaggacg
    10261 agggaggccc gccggatgcc gttgacccac tgcgaaggcg tcaggcagcg tgtgcacacc
    10321 gcatcccgga acgggtgatc ggaaaacggg atcaccgtat cccatccgaa ctgcctcacc
    10381 agcacccggt taatctcgga aagccgtacg cagggcgtcc agcggtattc acattggaac
    10441 agcacgttgt cccctagcgt acacggttcg tcctctgcga ggcggcgcca ctcctcgtcc
    10501 tccagcagga acgtgggatc aggcagccac tgggctgtaa acccgaggtc cgcaacaatc
    10561 ccctgtccgc tcttctcacg cacgctgagc gcggtgaact tttgcaggca cgcccgtatg
    10621 tcatcgcgca cggcaggctc caatgtctgc gcgccccagc tcgccgcgta cgaaatgcgc
    10681 cgcgccgtaa cctctccgaa atcgagaaaa aagtactttc gcagctcgct gtccgcgcag
    10741 gcccgtcggc tccagacctg gtcgctcccg cacacgtaag cgtcgaagcg cggaggctgc
    10801 cggagaaacg cgtcaacccg gcaagccgtt acgcccggcg acctgaaatg cttcgcacgg
    10861 aaagaggcga actttttcgc tttgacggcc tccatcacgg ttttgaccgg acgctccaac
    10921 acttttaatg gagagttccg ccgcttcgcg taatccacgg gcaagacggc gctctcgtgc
    10981 cccattctcc gcaaatatgt ttggagagca taggcttgca gaacgccgcc ataattgttg
    11041 gcctcgcaga gcgttacaat tccgatcttc acatcccccc cctctaggtt cagcttgcgg
    11101 tcatatcatc ccgagcgcat gcttcacaaa aagcttcacg cgccgccgga aagaaagttt
    11161 ggccatgcgc agcagaagcc ctcccagttc gtgcgtctcc agccgtgcga aaaaacgcgc
    11221 ccggtccgga tgcggccggg aagaccgcac aatcgaaggg ttacaagcga ccgcatgcgc
    11281 gtaatcggag tcgcgaaatt ccacgccgga ggcgcatgac cgaaaagcct ccacgccttt
    11341 cgcgctgttc gccagtacga gcgaggtgcc ccggttatcc cgaaaacagg gaaagcgctt
    11401 ttcaactccc cagtaatccg ccagcgtcag gtcgctgccg cttttcatct ccttgaaccg
    11461 gcaggcgtgg caggcggggc gcagaaagag gttggaaagg aagccgcgca gatacagctc
    11521 ctcctttacg gtctcccgat acgtgcggcc gtccgcaaaa gtggccgcaa aggagaactg
    11581 acgccagccg caggccttgt ttctgaaaga aatcccggtg attgcggaac ggctgcgctt
    11641 ttccaaacgc cgcttccaga gttcgaacac ccggggtgag gggacgccgt ggcagacgaa
    11701 atcgacgcac agcagtcggt caaacgcctt gcctaagaaa gcgcgcaggc ccgccacctg
    11761 acagggcgag ccggagaaga gcacctgccg cccggcccca agatgctgcc gtacgcgccg
    11821 aaatatatcg ttcatgtcgc tttgcacata cttcgatccc cgcaggcgcg cgaggtcggc
    11881 cgcatcctca acctcctgat ggacaacgcg aaacgttgca tcgcagcctg cgccgaaaac
    11941 gacacccccc cgctctagga cacgctccgc caaaagcgag aagaccccgc ccgacgaact
    12001 ctcctcacgc acggcagcgc aggtgttttg cgcagcataa acggcgacgg gttcgcgggc
    12061 cggcaggccg ggatggagcc ccgggcagat attcgtacag agcccgcagc gtaggcaccg
    12121 cgcagaatcc acgtgcggcc gtaagaaccc ttcgcggtcg ggtagcaggg cgacgcagga
    12181 ttccggacag atggcgctgc acgccgaaca acccgtgcag tcctctgtcg cacacagcag
    12241 taacggttgt gtgttttcat cgccgtgcac cggattcaga ttcataagta aatttacctt
    12301 agcatatccg taacggtgtc ttcaagcggc caaccttgtc gggatggcca gcaaggactg
    12361 ggctgagatc atcgcgaaag ttgaacagga cgcgaacgga ctgggggcga tagggcgcaa
    12421 gcgccctctc catctcgttc ccgtcggcgt tcagataaac ggtgcagagt gcgagcgcta
    12481 atcacgcacg tttgtttttc aaaccagacc cctttgaaga tcgtcaactc cgtcagcgaa
    12541 tactgaacac cactcccttg ggggacacat gaattgcaat cgcgttgcct gtcttgacaa
    12601 gcttgtaggt cacgaggccc ggcgcatcgg agttgtcgag cgtccaccgc gagatgtccg
    12661 cagtcagctg cgtcgccgtg atgaacgggt agagtccggt cggcagttta agcgaagcag
    12721 gcgcggtgag cgtgaccgcc gcatagtcgg gcagagagag atccccgttc accgtgagcc
    12781 ggttccacga gtcggcagcc gagaacgcca ccttgtagtt ccacggtgac tgcgccgttg
    12841 cggtcacctc cattggtgcg gacaccgtca cgtcgccgcc ggggagcact gccgtgcgga
    12901 tgcggctttc ggattccaac gcgagtgatg caccgtccgc cacggaaacc gactcaagat
    12961 ccgcagtcat gacggctccc tcgccgatcg cccgccactt tttcagcaga tagttgtgga
    13021 tgccctggcg gcgtccgtca tccagaagct tatcaaagat caccgcctcc gcgatccggc
    13081 agccaccgta gcgcagtccg ttgcggtcaa tacagaagta tgcgccctcc accatgtccg
    13141 ccgcgttcgt cgtcacaatc gtcagcacat ggaacccggg gttttcagag aagttatact
    13201 tgttggtggt caccgtgttt tggtcaatcc aagtcaggat ctgcgaatac caagggaaga
    13261 tggtggtata attcggcgca cgggcgccga aggtggagtt cttcgcgccg atgaaggatc
    13321 cggtggactc gccctcatga tcgcagaaca caatgtagca ttcgcgcaga ttcgggcagg
    13381 tcacctgtga tccggcggcg ttgtaccagc acatccccga actggtgttt gtgacggtcg
    13441 ccacatacag cgtagtgccg gcaggtttcg caccctcaac ggaatccaga aaatccacgc
    13501 acgggagccc ctcaacaaga ccgggatatg tcgcggtgcg gtagactgcg tttgtgaggc
    13561 cgcggtcttc actgggcagc gccgcgtaca tcccgttccc gcgcgtgtcc ttccacttga
    13621 cgatctgatc ctccattccg ggctctgtgc ggaactcgaa gctggaggca tcggaggcat
    13681 cgacatgcag aaaggcgtct gacataccgt caattcctgt ggagagcgag ccgccttcca
    13741 cgcggatcga tgcaacgtca tgcgggagcg tgcccacgtc gaggctgccg gagccgctct
    13801 tcacaaacga aatccctgtg ccgttgatga cccccacgcg caaggagcct tccacgtcca
    13861 actccggcgt ggcgccctcg gcgtagtcga tcgacgccag tgcccgcccc tcttgtgcgt
    13921 gcacggcgtc cggatgctcc cggttcagcc acttcttcag cagataggct tccgtggcgc
    13981 ggcgctcaag aggcgtgagt ttgtgcggat aggtgagcgc ctcgccgatg gcgatgccgc
    14041 cccatgtgcc gctgccatag ccgggcggca gaaacgcgaa attctgcacg ccgctgccgc
    14101 ccgcaccgta ggccgggccg aaaacgggga gactgtagcc gatcacataa acctgaccgg
    14161 gttccagcac gaacgagagg gggtccacgc gctcgccgtt gacggtccac acggcgcctt
    14221 gaacatggcc gtacgcgtcg tagcccaaaa gccggccctt atcgccgggc atgaaatcga
    14281 cggttccgac cgtaatgact cggtttgtgt tcggggagat gaccagatat ccttcgtggt
    14341 tgtaatacca cggtgcagca tcttctattc tgatatacat tgccgaggca tcctttggct
    14401 ccgcccacac ccagaagttt gtcttgttca acgtgagatt cgtacgttgg ggcccgaagt
    14461 ccatcatgtc gcgcccgttc gctgccgcgt aagccttacg gatcgtgggg aaacagctat
    14521 cgccgacatt gacgtagtta ccggcggtgt tgatgcgccg ggcgacaacg gtgtggccat
    14581 agaggttggt gccatcgcgg cggtccaccc attggctcac atattccatt tccgtgtcga
    14641 agacgttcgt tgtaaagaca ctcacgccgc agttggcgtc aaaccacgtt tccggcgcaa
    14701 ccggcggctc gtcaggcgcc gtgaatgagg cggttacaca ggtgtccgcg aaatcgagcc
    14761 tgccgcctgt cacgttgact ttggggagcg tctgcccggt caacttcttc acgcggagtc
    14821 cgcctgcccc ctgtttcacg aaggtggtgt cattaagatt cacctcgtcc accagcaccg
    14881 agccgctatt gacctttaat tgcgcaccgg aattgagcgt cacgcggccg aagtcgagat
    14941 ccgcggccac ctcggcgggt tgccatttcg cgcgcaggta tgcgttcacc cggcggcgtt
    15001 ccgccgacgt gagggcattc gtgtagatca gcacctcgcc gaacgcaatc ccgcccgtat
    15061 ggccatctac ggtcgaggcg aaacgatcgg cggccacggg atgcgccaac cccaccgaga
    15121 gcacgttcag atggcgggag taatcatcaa catcctcggg ctcgatccgc acgccgttca
    15181 ggcgcacatc agccgaggcg agaggctgcg tgagcttcgt gccggaagtg gtgacgctga
    15241 tgtcgaagag gccgccgtaa tagtaggtct ggtttacgcg cttggaaaag cggcgcgtaa
    15301 aatattgacc ctcaccgact gcgcggcaga gaaaattctg caggcagacg atccccgtat
    15361 tggaggggac gggctttttt gtgggagcat cgcggaacac cacgaacacc tcgcggatat
    15421 ccgaacgctc gccggcgttg aaaatcagtg tgccggccgg gccgtaggcc ttgaccgact
    15481 cggtgagcgg actgaacccg ccgaaatcga tgatcggcag gccgctcacg gcgtccgtct
    15541 caagacgcgg gcactgcgcg gcaccgtcct gtatcgcggc gagtccgttg gcgcggacat
    15601 cggcccacga cgtgacgtag ttggtgccgt cctggagggt caacgtgagc gaggcggagg
    15661 acgaggcgtc gagatgcatc cacggatcgc ccgccaaggc gggggcctcg taggaacggc
    15721 cgtttacggt aaacgtgccg tcaaccacat gcggatcggc gccaggtccg ccttgggaat
    15781 cgataacctc gatctcggcc gccccggttc cgtgcgtggt cttgttcgca aagttcacga
    15841 gatagcggcc tcgcccccaa gcacgctcga tcacggcttt cgcgccagcc gtgcgcacca
    15901 gaaaggcggg gttgctgatc atgcgcgcgt tcgccacggt cgttgtccaa gcgttgtcgg
    15961 agagaagcag ccaggttccg ccgataccgg ccgcaacccc cagcgaaccc tcgaaagacg
    16021 aggcgtcccg gatcgttgtg taacggtggg aaaacgagtt aacgcagttc agcacatggc
    16081 tggcggaacc taaaaccgtc cccagccagg cgggaacgtc gtagctggcc tccaaatctt
    16141 tgatctcttg ccggatagcg aggtcgccgt tgaaaacaaa tgtggaattg gtcatggcag
    16201 gcgtatcttt gatcagcggc caatcgccgt tttcccagtt ggcatccgtg ccccaaccgg
    16261 gtgttttggc tgtcgtgtta atccaattgt aggtgcccac ctcggacgtg ctggcgaacg
    16321 tgatggaagc ggcaccgcat atcgccaccg cgaggcacat gatcatcggt ctgccgagcg
    16381 cgtaacggca cctcctgatg tttatccgaa accgacttgc caaccccgtg cattcgagcc
    16441 gaactgtttt catcgtttta tcctcttttt cagacaatgt cttttgtcag ttgcttgtat
    16501 tgtactgtag ccccagtgta tttggcaacc ctcgcacttt tggttttgta atcagttcgt
    16561 gacaggaggc atgcgagtcg agcgcgcgtt ttgtgcgtac gcaaagagag cgcgggcgtc
    16621 ttccgcgcta cgcgcggatg taggatttat ttaggagtta ggatttagga tttaggattt
    16681 agcctgccgc gcggctcagc cgcgaaaaaa ccttggaata agtttaggac atgattaaca
    16741 tgatgttgtt caatcctgga aatcctgtca tcctgtcaat ttatagggca tgattttccg
    16801 ctgcgcgggc gcatatatcc atgattaaca tgatgttgtt caatcctgaa aatcctggat
    16861 acatgcgccc gcgcagcggg aaatcc
//
LOCUS       DAOSRB010000044        16436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3629_length_16436_cov_6.473659, whole genome shotgun sequence.
ACCESSION   DAOSRB010000044 DAOSRB010000000
VERSION     DAOSRB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16436
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_16436_cov_6.473659"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLZ74_03895"
     CDS             <1..587
                     /locus_tag="PLZ74_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97495.1"
                     /translation="RDNLAGDSLAGTTIEAAYEGLSVNVWYATSPDTDYREVNIGTAY
                     TLEWNDWEAYVSYTFLRFLSDKENDNEVGAGLSYSGLPAGFAAGVDGYYSFDAEGAFF
                     ETYINRECEVADWLTLTPAAVMGWNAGYMADGHDGANHLALVLEAAVPLKAGLELAGS
                     IAYSFAINDNADKYEEDDVLRSMFYAGVALNASF"
     gene            complement(848..4207)
                     /locus_tag="PLZ74_03900"
     CDS             complement(848..4207)
                     /locus_tag="PLZ74_03900"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOR97496.1"
                     /translation="MFFVACFCGSAAGAGTLDEDFARPPDTARCRTWWHWQDGCINPA
                     MMEKELDALARAGFGGVQMFVGGNCRIPKEEPRTPFLNEDWMKAVRRALVRSAAHGFD
                     FGFQNGAGWTGVGGPWMTTTNAMKHVFCVEKRVKGGEEAVLPKLADWPIERDPYWNYL
                     HNPWYGDIRAFAFPTPPAMLTPDLPLPALAASCDAPNLTNLHLRATYNIYGREKYPAA
                     LKYEKRENERDKADPWITFTFPEPVTVRTIAIKSSTMGDEIPSDAPYVWASDDGRDFR
                     FVAHLETGTAMAEDWFTSQEHPIEPTRAKVFKFTWETPCHLELREVHFSSRPSLTALQ
                     SQTGAATWNIATRRFIAEEAGTVVDPKDLIDVTSHIGVDGTLRYTPPADGRTWTVLRV
                     GATSTRRCNQPSPRDATGPACDLLDADAIRFHLSQYAQRILDEEKAAGVKTVRSLLLD
                     SWEQGTQNWSANLPAEFERRRGYALWNYLPIYAGYLVGSRDTSERYLWDARKTVNEVV
                     CENFFGVVRDWCRERGLQHYVEGFACGVGTFAGDAMTAYFHCDVPMTEASCTMREAPS
                     AAHLRGLPIVAVEAHTSAADWSVTPRDLKRREDAFFRAGMTRLIYHSYGHNPMPERRF
                     PGPTFAGYGVALQLGQTWWEMARPWQEYLARCQALLQRGAFVADVLAFSGETYGGRIT
                     GLYGPGCDGGMYSSDKLRGLPPGFDFDVVNADFLVKHIEVFPDGTLGAKDCPGGTRYR
                     VLVLRPEDKYMSIEVARKVRDLVKAGAAVVGPKPVCSLGLSRAVENDAEVRAIAAEVW
                     NGGRYGKGRAIETCSPDTGNSKNQHSAAVAVKSLGLKPDFVAKGAELVTQLSYLHRRE
                     GDVDIYFVCRWRKVPAGAEMGFRVTGKRPEIFDPLTGRTYVAKNFREEDGYTWLPAIE
                     GSASEASRFVVFRNSSSTAPAGVQRPNEGVRTEFPVTCGDWKIHFKDAFGVDRTVVTN
                     GLFDWTDSADEAIRTFSGIATYRTTLTLPSAALGEKPGARFLLALGTVDELAEVFLNG
                     QSCGIAWCEPYEVDITAMLKRSAGSASGRPAAILNIEVRVANAWLNRMLADMQKPEAE
                     RKTWTQRMPLPDAKPEPSGLKGPLTLVRVEE"
     gene            complement(4337..6037)
                     /locus_tag="PLZ74_03905"
     CDS             complement(4337..6037)
                     /locus_tag="PLZ74_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97497.1"
                     /translation="MKNLSVVVVAIVAAVVASAKDYNWAAAQDGTFGTSANWSPNTGV
                     PGEDDNAVFDVPGTYCVSEVEASRTIGSVSVTNANVIMALGGNTWTIKGPWATYNQAS
                     DAAITSTTITNGTLDVQHGSSSGASGIIISAKDSSVTKGNLRITGDNTHVTTKALWMG
                     GTRGSFILDGGATCDVAGEVRWGAEYGGGSDNLVVITGEGTRFRQMAAGATTGNNIGD
                     RCKYYISDGASAYFTSMYHLAGGYGGRWNELWITNGASVVYGGGFAVGSAANGRPIHQ
                     NVCTIAGMGTRVTVTGRLDVATAAISNLLHITDHAVVTQTVATGTTSSYVGYDGANAG
                     QGCGHEFRVDGGAEYRCVPSPSASYHYLYVGGGVYSTGNRIFVGEGSLFEIARVNNNG
                     MIGYNGAYNNGIVVSNGTFNISTSWPISFGTSKNESTPCGSGNYVEFYGDHPLAYMGQ
                     LTFNTGSKLIYHIGANGYAAAPMNFRYKAQNTSLTPARLVLDVSEWRPDRKTTIPLVQ
                     TMRDGDVTANKVLLDELKDNVSVTGTKHPEWYTVSLSADNKTLQLTCKPIRELILMIL
                     "
     gene            complement(6188..7576)
                     /gene="secY"
                     /locus_tag="PLZ74_03910"
     CDS             complement(6188..7576)
                     /gene="secY"
                     /locus_tag="PLZ74_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOR97498.1"
                     /translation="MAMLATFANTFKIPELRKRILLTAALVFISRLISMIPTPGVDWQ
                     TLQAELARNREIAGAGGGLMDWFNVFSGGALNRCAVGFLSIWPYISASIIMQLLTAVL
                     PMLEKLRQEGESGRMKLAQYTRYLTIGICVFQSFALATALQNPASFGLGAVQIVTRPG
                     LGFKLMTVISMTTASLFVMWLADQITQRGIGNGTSLIIMINITSRLPMAIIEAWTRYF
                     GLGGITATKSPTELILLLLFGFLITMGTVMLTQGLRKVPIHSTRRSAGGGRNVYGMQQ
                     TYMPLRVNYTGVMPIIFAGPLIQFPAVLLSKIPAEITWLSGLKWLGSQLVDMTSPIHL
                     AAYALLIMFFSFFWVATQFNAMQISDNLKRDGSYVPGIRPGRATAEYLDALMTRVTLI
                     GGVGLLIIALVPQLLRGLMDLPWEMASFFGGTSLLIIVGVALDTLRQMESHLLMRNYD
                     GFLKHGRLRGRH"
     gene            complement(7594..8034)
                     /gene="rplO"
                     /locus_tag="PLZ74_03915"
     CDS             complement(7594..8034)
                     /gene="rplO"
                     /locus_tag="PLZ74_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOR97499.1"
                     /translation="MDLSSLKNTPGARKSRKRVGRGMGSGMGKTSTRGHKGQQARKGH
                     KYRLGFEGGQMPLIRRLPKRGFKNPNRVEYFALNVRELERFEDGTEVTLELLHANGLF
                     ANKYDGVKLLGSGELTKKLTVKVNGASASAKAKVEAAGGSVEIV"
     gene            complement(8114..8587)
                     /gene="rpsE"
                     /locus_tag="PLZ74_03920"
     CDS             complement(8114..8587)
                     /gene="rpsE"
                     /locus_tag="PLZ74_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOR97500.1"
                     /translation="MSRNEGSSEFDERVVFVNRCSKVVKGGRRFSFSAVVVVGDREGR
                     VGFGFGKANEVAEAIRKGGDSAKKDMIRVKLDGKTIPHAVTGVCDGGRVLLKPAPKGT
                     GLIVGGGMRPVLEVAGVYDAIGKSLGSKNRLNVVKATVDALNQLRSAEEIAAARA"
     gene            complement(8602..8982)
                     /gene="rplR"
                     /locus_tag="PLZ74_03925"
     CDS             complement(8602..8982)
                     /gene="rplR"
                     /locus_tag="PLZ74_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOR97501.1"
                     /translation="MSFNRKKQRARRHMRLRQRIQGTAERPRMAICISNKHMYVQFID
                     DTAMNTLASTSTLKEAKPCNLETARQVGEQAATVAKEKGISIVVVDRGGFKFHGRVKQ
                     IVDAATAAGLRISDRVTEVAEEAK"
     gene            complement(9006..9545)
                     /gene="rplF"
                     /locus_tag="PLZ74_03930"
     CDS             complement(9006..9545)
                     /gene="rplF"
                     /locus_tag="PLZ74_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOR97502.1"
                     /translation="MSRIGNMPIAIPEGVTVTAQGNAVTVKGKLGELSRVFQSGIDVR
                     VEDGQVVVTRADDSRMQKSCHGLSRTLVRNMIEGVTTGYKKVLTIEGTGFKAALQGNR
                     LSLSLGFASPKDYLIPDGIRITVDAAGLNVTVEGISKELVGQAAARIRNYYPAEPYKG
                     KGIKYADEQIRRKQGKTVA"
     gene            complement(9559..9951)
                     /gene="rpsH"
                     /locus_tag="PLZ74_03935"
     CDS             complement(9559..9951)
                     /gene="rpsH"
                     /locus_tag="PLZ74_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOR97503.1"
                     /translation="MTSDPISDMLLRIRNGLKAKLPSITLPGSRIKGEIARVMKEEGY
                     IADFTLTAAVPRALRIELKYNDDAEPAIRGLRRESKPGLRKFCGVQEIPQVLGGMGVA
                     ILSTSGGILSGKEARKRKLGGELICTIW"
     gene            complement(9963..10148)
                     /locus_tag="PLZ74_03940"
     CDS             complement(9963..10148)
                     /locus_tag="PLZ74_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOR97504.1"
                     /translation="MAKTCLMEKAKRKPKFSTRKYYRCSRCGRPRAYIRKFQLCRICF
                     REMALSGMIPGVTKASW"
     gene            complement(10163..10702)
                     /gene="rplE"
                     /locus_tag="PLZ74_03945"
     CDS             complement(10163..10702)
                     /gene="rplE"
                     /locus_tag="PLZ74_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOR97505.1"
                     /translation="MARLKETFAKEMVPKLQEKLGIKNKMLVPRLQKVVLNMAFGIVE
                     KDVQKTIVDDLAKLSGQKPVLCKARKSISNFKLREGMVIGAKVTLRGDRMFDFMERLV
                     NSALPRIRDFRGVPSRGFDGRGNYTLGLKEHTIFPEMIDSGTPGADVGMDITFVTSAK
                     DNQGARELLGILGMPFTGK"
     gene            complement(10717..11034)
                     /gene="rplX"
                     /locus_tag="PLZ74_03950"
     CDS             complement(10717..11034)
                     /gene="rplX"
                     /locus_tag="PLZ74_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOR97506.1"
                     /translation="MSVARIKKEDTVIAIAGDYAGKTGKVLRVMPKKGKAVVQGLNMV
                     KKAIRRSQTAPEGGFIEREAPIDLSNLMPYDPDAKKGVRIRRVKEGDKVVRQSKATGK
                     NLD"
     gene            complement(11119..11484)
                     /gene="rplN"
                     /locus_tag="PLZ74_03955"
     CDS             complement(11119..11484)
                     /gene="rplN"
                     /locus_tag="PLZ74_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOR97507.1"
                     /translation="MIQEQTVLTVADNTGAKRAMCFRVLGQRKRYARLGDVIRVAIKE
                     ATPTGAIKKGQVCTAVIVRTGQPIRRSDGSYVRFDQNACVIVDNKMNPIGSRIFGPVA
                     RELRDRNYMKIISLAPEVV"
     gene            complement(11486..11758)
                     /gene="rpsQ"
                     /locus_tag="PLZ74_03960"
     CDS             complement(11486..11758)
                     /gene="rpsQ"
                     /locus_tag="PLZ74_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOR97508.1"
                     /translation="MAGTTEAVQTRGSRKVRKGKVISKSGRKSVIVQSERRERHPVYG
                     KVIRRFKKYHAHDESDTVQVGDAVTIMETRPLSATKRWRVVKPEGK"
     gene            complement(11759..11953)
                     /gene="rpmC"
                     /locus_tag="PLZ74_03965"
     CDS             complement(11759..11953)
                     /gene="rpmC"
                     /locus_tag="PLZ74_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOR97509.1"
                     /translation="MKARDLRELGTAELDQKIRELSQELNDLRIKHKSGAGIEKPVRM
                     RLMRRDIARMKTIRTQREAK"
     gene            complement(11962..12384)
                     /gene="rplP"
                     /locus_tag="PLZ74_03970"
     CDS             complement(11962..12384)
                     /gene="rplP"
                     /locus_tag="PLZ74_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOR97510.1"
                     /translation="MPLMPKRVKHRKQFKGNRAGHATSGAQLAYGEFGLKALERGWLT
                     NTQIEACRVVINREMKRKGKLWIRVFPDKPVTRKPIEVRMGGGKGNPELWVAVILPGK
                     MLFEIGGVPDSVARECLRLADTKIGIRTTLVTRHVTGV"
     gene            complement(12368..13045)
                     /gene="rpsC"
                     /locus_tag="PLZ74_03975"
     CDS             complement(12368..13045)
                     /gene="rpsC"
                     /locus_tag="PLZ74_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOR97511.1"
                     /translation="MGQKVNPVGFRLAVDKEWRSRWFANKKQFGELLAEDLMIRELVK
                     KRLESAAITKILIERYANRVRINLFTARPGVVIGRKGQDVDRIRQDLAKKTGKEIYLE
                     IHEVRDPDANAQLVAESIAQQVERRVALKRAMKRAEKVAMDMGVDGIKIRCSGRINGA
                     EISRVEWSKQGKVPLHTLRANIDYGFAEAYTTAGLIGVKVWICKKDDFQPVRAGGQRR
                     RRDASHA"
     gene            complement(13077..13412)
                     /gene="rplV"
                     /locus_tag="PLZ74_03980"
     CDS             complement(13077..13412)
                     /gene="rplV"
                     /locus_tag="PLZ74_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOR97512.1"
                     /translation="MEVTAITRNVRMSAKKGRPLARKCQGLPVAAALNIVEFSPRKAA
                     TILSKTLKSAVANARNNANLDVDTLKVSLCVFDEGSRLRRFWPRARGSASPIAKRLCH
                     CKVVLTDGQ"
     gene            complement(13427..13708)
                     /gene="rpsS"
                     /locus_tag="PLZ74_03985"
     CDS             complement(13427..13708)
                     /gene="rpsS"
                     /locus_tag="PLZ74_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOR97513.1"
                     /translation="MARSIKKGPYVEKSLLDKVEKMNASGRKQPIRTWSRRSMILPEF
                     VGHTFAIHNGKQHVPIFITENMVGHRLGEFAPTRTFRQHGVHTDKAVAK"
     gene            complement(13712..14536)
                     /gene="rplB"
                     /locus_tag="PLZ74_03990"
     CDS             complement(13712..14536)
                     /gene="rplB"
                     /locus_tag="PLZ74_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOR97514.1"
                     /translation="MGLKTFKPRSAGLRHRVSPTFDEITEVKPLRRLTEARKRKAGRN
                     NQGRITTRHRGGGAKRRYRIVDFRRNKIGIPARVEAIAYDPNRSANLALLKYVDGEKR
                     YILAPAGLKVNDAVMAGPEAEPNVGNAMPLGQIPLGMTVHNIEMIPGQGGKLARSAGN
                     GCQVMARDGDFVTLRMPSGEIRLINARCMATIGQVGNAEWGSIKLGKAGRKRRMGIRP
                     TVRGVAMNPVDHPMGGGEGRTSGGSHPRSPWGKLAKGGKTRSRRKTSSKFIVKRRK"
     gene            complement(14560..14838)
                     /gene="rplW"
                     /locus_tag="PLZ74_03995"
     CDS             complement(14560..14838)
                     /gene="rplW"
                     /locus_tag="PLZ74_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOR97515.1"
                     /translation="MKHAGIIRKVQITEKGTRLSCENRYLIEVAPKANKIEIKEAVEK
                     QFGVHVLAVNTQNYKGALRTLKNRRVVRDPSWKRAIVTVKAGERIETV"
     gene            complement(14835..15485)
                     /gene="rplD"
                     /locus_tag="PLZ74_04000"
     CDS             complement(14835..15485)
                     /gene="rplD"
                     /locus_tag="PLZ74_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOR97516.1"
                     /translation="MSTIKVFNPAGEPAGEMTFAKERLELEKGAQAVKDVVVAIRNSA
                     RAGTASTLSKGEVAGSNRKPWRQKGTGRARAGYRQSPVWRGGGVAFGPKPRDYSQKIN
                     RKVQQLAFTRALSDVIAEGRVAVIEKFDLTGPKTKQMTAMLKKLGADRSVLIVLDAYD
                     ENVVLSARNLQKVEVVTAAEVTVYSLLLYRKLIATQAGFEALTARMRPVKKKEVDE"
     gene            complement(15500..16126)
                     /gene="rplC"
                     /locus_tag="PLZ74_04005"
     CDS             complement(15500..16126)
                     /gene="rplC"
                     /locus_tag="PLZ74_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOR97517.1"
                     /translation="MEGLLGRKIGMTQVFDDQGRRIAVTVLEAGPCPVVQVKTPETDG
                     YRAVQLGFEEQKPSRLSKAQVKHFEKAGVKPCRLTREFALDDGEEVKPGEMVTVTMFD
                     GVKYVDVSGVTKGRGFAGVIRRYRIGGGAHTHGGHSKHRIGSIGCREFPGTVHKGKRM
                     PGQMGNVNRKARNLTVVQVRGEDNLLLVAGSVPGPKGGVVMIRKALKK"
     gene            complement(16140..>16436)
                     /gene="rpsJ"
                     /locus_tag="PLZ74_04010"
     CDS             complement(16140..>16436)
                     /gene="rpsJ"
                     /locus_tag="PLZ74_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOR97518.1"
                     /translation="RIRIRLQAYDHRVLDQAVSDIIDTARGTGAQVVGPIPLPTRIER
                     FTVNRSPHVDKKSMDQFEMRTHKRLLDIVGPTAKTVDELKKLNLPAGVDITIRI"
BASE COUNT         3054 a         5428 c         4480 g         3474 t
ORIGIN      
        1 gccgcgacaa ccttgccggc gacagtctgg cgggcacgac gatcgaggcc gcctatgagg
       61 gtctctcggt caatgtgtgg tatgcgacaa gccccgacac ggactaccgc gaggtgaaca
      121 tcggcacagc ctacaccctc gaatggaacg actgggaggc ctacgtctcg tacacgttcc
      181 tgcgtttcct gagcgacaaa gagaacgaca atgaagtcgg cgctggcctc tcgtattcgg
      241 gactgccagc cgggttcgcc gccggagttg acggctacta ctccttcgat gccgaaggcg
      301 cattttttga aacatacatc aacagggagt gcgaggttgc ggattggctc accctcacgc
      361 ccgcagcggt gatgggttgg aacgcgggct acatggccga cggccacgac ggcgcgaacc
      421 acctcgcgct ggtgctggag gcggccgtgc cgctcaaggc ggggctcgaa ctggcgggga
      481 gcatcgccta ctcgtttgcg atcaatgata acgccgacaa atacgaggaa gacgatgtcc
      541 tgcgcagcat gttctacgct ggagttgctt tgaacgcttc attctaatgg acataaccgc
      601 acgcgagcca tagcgagtgg ttgttggctt caactttaca agctgaacca tttacggcca
      661 tctgttgcca tcagtttatc ggcggcggac ggcccggtcg aaccggcctc atacgtgtgt
      721 agcggcatcc ggtccggaca gtcgcgccat cggacacgac ggtcgccaac cccaatgcca
      781 acaccagccc ccgctttttt aaaacaaaca cgatcacgcc cctgtttctt ttattcttcc
      841 agaaccttca ttcctccaca cgaacaagcg tgagcgggcc tttgagccct gacggttcgg
      901 gcttcgcatc cggcagcggc atccgctgag tccacgtttt gcgttccgcc tcgggctttt
      961 gcatatccgc gagcatccgg ttcagccaag cgttcgccac ccggacttcg atgttgagga
     1021 tagcagccgg acgtccggac gctgagccgg cactcctctt cagcattgcc gtgatgtcca
     1081 cctcgtatgg ctcgcaccaa gcgattccac aggattggcc gttcaagaaa acctccgcga
     1141 gttcgtccac cgtgccgagg gcgaggagaa accgggctcc cggcttctca cccaaagcag
     1201 cggaaggcaa cgtaagcgtc gtccggtaag tggcgatgcc ggagaaagtg cggatggcct
     1261 cgtccgccga atcggtccag tcgaacagac cgttcgtaac cacggtgcgg tccacaccga
     1321 acgcatcctt gaagtgaatc ttccagtccc cgcacgtaac ggggaactcc gtgcgaacgc
     1381 cttcgttggg tcgttgaacc cccgccggtg ccgtactgga ggagttgcga aaaacaacaa
     1441 accgggacgc ctcagaggct gaaccctcaa tggcgggaag ccaagtgtaa ccatcttcct
     1501 cgcggaagtt ttttgccaca tatgtgcgtc ctgtaagcgg atcaaagatt tccgggcgct
     1561 tcccagtcac gcggaaaccc atttcggctc ctgcgggaac ctttcgccaa cggcagacga
     1621 agtagatgtc gacgtcaccc tcacgacgat gcaggtagga gagctgcgtc acaagttcgg
     1681 caccctttgc aacgaaatcg ggtttcaggc caagggactt cacggcaacg gccgcactgt
     1741 gctggttttt agaattcccc gtgtcagggg aacaggtctc gatcgcgcgt cctttaccat
     1801 aacgtccgcc gttccacact tcggcggcga tcgcgcgcac ttcggcgtca ttctcaaccg
     1861 cccgggaaag tccgagcgag cagacgggct tcggaccaac aaccgcggca ccggccttca
     1921 cgaggtcgcg gaccttccgc gcgacttcga tgctcatgta cttatcctcg ggacgcagca
     1981 caagcacgcg gtagcgcgtg ccgcccggac agtctttggc tccgagcgtg ccgtccggaa
     2041 acacctcgat atgtttcacc aagaagtcgg cgttcaccac atcgaagtcg aagccgggcg
     2101 gcagtcctcg caacttgtcg cttgagtaca tcccgccgtc acaaccgggg ccgtagagcc
     2161 ccgtaatccg cccgccgtag gtctctcccg aaaaagcgag cacatcggcc acgaaagcgc
     2221 cgcgctgcaa gagtgcctga caccgcgcca gatattcctg ccacggccgg gccatctccc
     2281 accacgtttg ccccaactgg agcgccacgc cgtaaccggc aaaggtcggc cccggaaaac
     2341 gacgctccgg catcgggttg tgcccataag aatggtagat gagacgcgtc atgcccgcac
     2401 ggaagaatgc gtcttcgcgc cgcttgaggt cgcgcggtgt aacgctccag tcggcggcgg
     2461 aggtgtgcgc ctcgacagcc acaatgggta gcccgcgcag atgggcggca ctcggcgcct
     2521 cacgcatcgt gcaggatgcc tcggtcatcg gaacatcgca atggaagtaa gcggtcatcg
     2581 cgtcccccgc gaacgttccc accccacatg caaaaccctc cacgtaatgt tggagcccgc
     2641 gttcccggca ccaatcgcgc accacgccga agaagttctc gcacacaacc tcgtttacgg
     2701 tcttgcgcgc atcccacaga taacgttcgc tcgtgtcccg gctccctaca agatagccgg
     2761 cgtagatggg cagataattc cagagcgcat acccacgacg ccgctcgaat tcggcgggca
     2821 gattcgccga ccagttctgt gttccctgct cccaactgtc taacaggagc gaccgaaccg
     2881 ttttcacgcc tgcggccttc tcttcgtcga ggatgcgttg cgcatactgc gagagatgga
     2941 agcggatggc gtccgcatca agaaggtcgc acgctggccc tgtcgcgtcg cgcggcgagg
     3001 gctggttgca gcggcgcgtg gaggtggccc ccacccggag cacagtccat gtgcgtccgt
     3061 ctgcgggcgg tgtgtagcga agcgtgccgt cgacaccgat atggctcgtc acgtctatca
     3121 gatctttcgg atcgacgacc gtgcccgctt cctcggcgat gaaccggcgg gtcgcgatgt
     3181 tccacgtagc cgcgcccgtc tgactctgaa gggctgtcag agaaggcctc gacgaaaagt
     3241 ggacttcgcg gagctcaagg tggcaaggtg tttcccatgt gaacttaaac accttggccc
     3301 gcgtcggctc gatcggatgc tcctgactgg tgaaccagtc ctcggccatc gccgtgcccg
     3361 tttccagatg cgcaacgaat cggaaatccc gtccgtcatc ggaggcccac acgtagggcg
     3421 cgtctgatgg aatctcgtcg cccatcgtcg aggacttgat ggcgattgtg cgcaccgtga
     3481 cgggctccgg gaacgtaaac gtgatccagg ggtctgcctt gtcgcgttca ttttcccgct
     3541 tctcgtattt caaagcggcg ggatattttt cccgcccgta gatattatac gtggcacgca
     3601 gatgcaggtt cgttagattc ggcgcgtcgc acgaggctgc cagcgccgga agcggcaggt
     3661 ctggggtgag catcgccggc ggcgtgggga acgcaaaggc gcggatgtcc ccataccacg
     3721 gattatgcag ataattccaa taagggtccc tttcgatcgg ccagtccgcg agcttcggca
     3781 gcacggcctc ctcgccccct tttacgcgct tctccacgca gaagacgtgt ttcatcgcgt
     3841 tcgtggtggt catccagggg ccgcccacgc cggtccagcc cgccccgttc tgaaacccga
     3901 agtcgaaacc gtgcgccgcg ctccgcacaa gggcgcggcg aaccgccttc atccaatctt
     3961 cattcagaaa cggggttctc ggctcctctt tggggatgcg gcagttccct cccacgaaca
     4021 tctgcacacc gccgaaaccg gcgcgcgcca gcgcatcgag ctctttctcc atcatggcgg
     4081 ggttgatgca gccgtcctgc cagtgccacc acgtacggca tcgcgccgtg tccggcgggc
     4141 gggcaaagtc ctcgtcaagc gtgcccgctc ctgcggcaga cccgcaaaaa catgcgacaa
     4201 aaaacagtga agaaacgagc gtgcctgttt tgatacgtcc cccccttcgt tcaacttttc
     4261 ctgccgttcc tcaaacggca ggaaaacatc caattttccg tatcaaggca tccgtcaggg
     4321 atgcgttgat tgcaaatcac aggatcatca aaatcagctc gcggatcggt ttgcacgtca
     4381 gttgaagcgt cttgttgtca gccgaaagcg ataccgtgta ccattcggga tgtttggttc
     4441 ccgtcacgct cacattgtcc ttcaactcgt ccagcaatac cttgttcgcc gtgacatccc
     4501 catcccgcat ggtctgcacc agcggaatcg ttgtcttgcg atccgggcgc cactcggaga
     4561 cgtccagtac gagccgcgct ggtgtcaagg acgtgttctg cgccttataa cggaagttca
     4621 tcggcgcggc cgcatacccg ttcgcgccga tatggtagat cagtttcgaa cccgtgttga
     4681 acgtcagttg acccatgtac gccaacggat ggtcgccgta gaactcgacg tagtttccgc
     4741 tgccgcaggg tgtcgattcg tttttggacg ttccgaagct gatcggccaa cttgtggaaa
     4801 tattgaatgt gccgttggaa acgacaatgc cgttgttgta cgccccattg taaccgatca
     4861 tgccgttgtt attcacgcgg gcaatctcga ataacgagcc ttcgccgacg aaaatacggt
     4921 tccccgtgga atacacgccg ccgccgacgt aaagatagtg ataactggca ctcggcgaag
     4981 gaacgcaccg gtattccgca ccgccgtcga cacggaactc atggccacat ccctggccgg
     5041 cattcgcacc gtcatatccg acataggacg acgtcgttcc tgtcgcgacc gtctgtgtaa
     5101 ccacggcatg atccgtgatg tgcagcaagt tggagatggc cgctgtcgca acatccaatc
     5161 tgcctgtgac cgtcacgcgc gtacccatgc ctgcaatcgt gcagacgttc tgatggatcg
     5221 gccgaccatt cgccgcactt cccaccgcaa agccgccgcc ataaacgaca gacgcgccgt
     5281 tcgtgatcca caactcgttc caccgtccgc catagccgcc cgccagatgg tacatggatg
     5341 tgaaatacgc cgacgcaccg tcggagatgt aatatttgca gcggtcgccg atattgttcc
     5401 ctgttgtcgc cccagccgcc atctgacgaa agcgcgtacc ttcgcccgta atgacaacga
     5461 ggttgtcaga accgccaccg tattcggcac cccagcgaac ctcgcccgcc acatcgcacg
     5521 tcgcaccgcc gtcaagaatg aatgagccgc gcgtgcctcc catccacagg gctttggtcg
     5581 tgacgtgcgt gttatcgccc gtaatccgca agttgccctt tgtaacggaa ctatccttgg
     5641 cggaaatgat gatcccgctt gcacccgaag acgaaccgtg ctgcacgtcg agcgtgccgt
     5701 ttgtgatcgt cgtagaggtt atagcggcgt ctgacgcctg attatacgtc gcccacgggc
     5761 ctttgatggt ccacgtgtta cctccgaggg ccatgatcac gttcgcattc gtcacgctca
     5821 cggaaccgat cgtacggctc gcttccacct cgctgacaca atatgtaccc ggcacatcga
     5881 atacggcatt gtcatcctcg cccggcacgc cggtgttggg cgaccagttc gccgacgttc
     5941 caaacgtgcc gtcctgagcg gcggcccagt tatagtcctt cgccgaagcg acaaccgcgg
     6001 ccacaatcgc aacaactacg acactcaagt ttttcataaa caaacccttt ctaaacatca
     6061 tccattttta ttataagttg ttttttctca accgcaacct ctaaacaccg tctgtcgtgt
     6121 aattttcaaa agcaaaaggc ccgcagaccg gcaagtccac gggccttcat aatcaaccgc
     6181 gctcaactca gtgacggcct cttaagcgtc cgtgcttcaa gaagccgtca tagttgcgca
     6241 tcaggagatg cgactccatc tgacgcaggg tgtccagcgc cacacccacg atgatcagca
     6301 gactggttcc accgaaaaag gaggccattt cccaagggag gtccatcagc ccccgcagca
     6361 attgggggac caaggcgatg atcagaagtc cgaccccgcc gatcagcgtc acgcgggtca
     6421 tcaatgcgtc cagatattcg gcggtcgcac ggccgggacg gatgcccggg acataagacc
     6481 cgtcgcgctt caggttatcc gaaatctgca tcgcgttaaa ctgcgtggcg acccagaaga
     6541 acgagaagaa catgatcaac agcgcataag cagcgagatg gatcggactg gtcatatcga
     6601 ccaactgact gcccaaccat ttgaggcccg agagccaggt gatctcggcg ggtatcttgc
     6661 tgagcagcac cgccgggaac tggatcagcg gaccggcgaa gatgatcggc atgacgcccg
     6721 tatagttgac gcgaagcggc atataggttt gctgcatgcc gtagacattc ctcccgcctc
     6781 ccgcagaacg gcgggtggag tggatcggca ccttgcgcag accctgcgtc agcatgacgg
     6841 tccccatggt gatcaggaac ccgaagagca ggaggagtat caactcggtc gggctcttgg
     6901 tggcagtgat gccgcccagc ccgaaatagc gtgtccacgc ctcaatgatc gccatcggca
     6961 ggcgcgaggt gatgttgatc atgatgatca gcgaagttcc gttaccgatg ccgcgctggg
     7021 tgatctggtc cgccagccac atgacaaaca gggaggcggt ggtcatggag atcacggtca
     7081 tgagtttgaa acccagtccc gggcgcgtca cgatctgcac cgcaccgagt ccgaacgagg
     7141 ccggattctg cagggcggtc gcgagagcaa aggactgaaa cacgcagata ccgatggtca
     7201 ggtaccgcgt atactgggcc aacttcatcc tgcccgactc cccctcctgg cggagtttct
     7261 cgagcatcgg gagcacggca gtcagcaact gcatgataat cgaggcactg atgtaaggcc
     7321 agatgctcag aaaaccgacg gcgcaccggt tgagggcgcc gccgctgaac acgttaaacc
     7381 agtccataag accgccgccc gctcccgcga tctcgcggtt gcgcgcgagt tccgcctgca
     7441 acgtctgcca gtccacgccc ggcgtcggga tcatggagat cagacggctg atgaaaacca
     7501 gcgcggccgt cagcaggatg cgcttccgga gttccggaat tttaaaggtg ttcgcaaacg
     7561 tggcaagcat ggccataatc gtgatcgccc ttcctaaacg atttcaacgc tgccgcccgc
     7621 cgcctcgacc ttggctttgg ccgacgcgct ggcgccgttg actttgaccg tcagtttctt
     7681 ggtcagttca ccagagccga gcagtttgac cccgtcatac ttgttggcaa agagcccatt
     7741 cgcgtgcaac agttctagcg tgacttccgt tccgtcctcg aagcgttcga gttcgcggac
     7801 attcagcgcg aaatactcca cgcggtttgg attcttaaag ccgcgcttgg gcaggcggcg
     7861 gatcagcggc atctgtccgc cttcgaaccc gagcctgtac ttgtgaccct tgcgggcctg
     7921 ctggccctta tggccgcggg tggaggtttt ccccattccc gaacccatgc cgcgtcccac
     7981 gcgtttgcgc gatttccgcg ctccgggggt gtttttcagt gatgacaaat ccattacgta
     8041 aactccttta cggtaatcac gaatcgtgga tgcgggaaac ggggcatgct tcactcctcc
     8101 cgcttcaccc gtttcatgct cttgcggccg cgatctcctc cgccgaacgc aactggttca
     8161 gcgcgtccac cgtagccttg actacattca gccggttctt gctgcccagc gatttgccga
     8221 tggcgtcata cacgcccgcc acctccagca cagggcgcat accgccgcca acgatcagac
     8281 ccgttccctt gggcgcgggc ttcagcagca cacggccgcc gtcacaaact ccggtcaccg
     8341 catgcgggat cgtcttgcca tcgagcttga cgcggatcat gtccttcttg gccgaatccc
     8401 cccccttgcg gatggcctcg gccacctcgt tggccttgcc gaaaccgaaa cccacccggc
     8461 cctcgcggtc gcccacgacc accaccgcgc tgaaactgaa gcggcggccg cccttcacga
     8521 ccttcgaaca ccggttgacg aacacgaccc gttcgtcgaa ctcactcgat ccctcattac
     8581 gggacaattt cgcctctgcg ctcacttcgc ctcctctgcc acttccgtga cgcggtcaga
     8641 aattctcagt ccggcggcgg tcgcggcatc cacgatctgc ttgacgcgtc catggaactt
     8701 gaacccgccg cggtccacga ccacgatcga gatgcccttt tccttcgcca cggtggcggc
     8761 ctgctcgccg acctgccgcg cggtttccag attacacggc ttggcctcct tcagggtgga
     8821 ggtcgaagcc aacgtattca tggccgtatc atcaatgaac tgcacataca tgtgcttgtt
     8881 cgagatgcag atggccatgc gcggacgctc ggccgtgcct tggatccgct gacgcaggcg
     8941 catgtgacgc cgggcgcgtt gttttttacg gttaaaactc atctttcccc agctccgttc
     9001 aggcgttacg cgacagtctt gccctgttta cggcggatct gctcgtccgc gtacttgatc
     9061 cccttgccct tgtagggctc ggccggataa taattgcgga tgcgcgcggc cgcctgaccc
     9121 acgagttctt tgctgatccc ctccaccgtc acgttcaggc cggccgcatc aaccgtgatg
     9181 cggatcccgt ccggaatcag gtaatccttg ggcgaggcga accccagcga aaggctgagt
     9241 cggttgccct gcagggccgc tttaaacccc gtcccctcga tggtcagcac cttcttgtat
     9301 ccggtcgtca cgccctcgat catattccgg accagagtcc ggctcagacc gtgacaactt
     9361 ttctgcatgc gggaatcatc cgcacgcgtc acgaccacct ggccgtcctc gacgcggaca
     9421 tcaatcccgc tctggaagac gcggctcaat tcgcccagct tccctttgac cgtcaccgca
     9481 ttaccttgtg cggtcaccgt gaccccctcg gggatcgcaa tcggcatgtt accaattctc
     9541 gacatcgttg aagccctctt accagattgt gcagatcagt tcgccgccaa gcttgcgctt
     9601 ccgggcttcc ttgccgctca gaatgccgcc cgacgtgctc aggatggcaa cgcccatccc
     9661 gcccagcacc tgggggatct cctgcacacc acagaatttg cggagtcccg gcttgctctc
     9721 gcggcgcaga ccgcggatcg ccggttcggc gtcgtcattg tacttcagtt cgatgcgcaa
     9781 cgcgcgcggc acggcggccg tcagcgtgaa gtcagcgatg tagccctctt ccttcatcac
     9841 ccgggcgatc tcgcccttga tccgggaacc cggaagcgtg atggaaggca gtttggcttt
     9901 aagtccgtta cggattcgca acagcatgtc agaaatcgga tccgacgtca tggttctctc
     9961 ctctaccagc ttgccttagt cacacccgga atcattccac tcaacgccat ctcgcggaaa
    10021 cagatacggc agagctggaa cttgcggata tacgcgcgcg gacgaccgca acgggaacag
    10081 cggtaatact tgcgcgtcga aaacttcggc ttgcgcttgg ctttctccat cagacaggtt
    10141 ttggccattg tggggaatct ccttatttgc cggtgaacgg catccctaat atcccgagca
    10201 actcacgggc accctgattg tccttggccg acgtgacgaa cgtgatatcc atcccgacat
    10261 cggcgcccgg cgttccggaa tcgatcattt cggggaagat cgtatgctcc ttcagcccca
    10321 aagtgtagtt tccacggccg tcgaacccgc ggctcggcac accgcggaag tcgcggatac
    10381 ggggcagcgc gctgttcacc agacgctcca tgaagtcaaa catacggtcg ccccgcaagg
    10441 tgactttggc gccgatgacc atgccctcgc gcaacttgaa gttggaaatg cttttgcgcg
    10501 ctttgcacaa caccggcttc tggccggaaa gcttggccag atcgtcgacg atggtcttct
    10561 gcacgtcctt ctccacaatc ccgaacgcca tgttgagcac gactttctgg aggcggggca
    10621 ccagcatttt gtttttgatc cccaacttct cctgaagttt gggcaccatt tcttttgcga
    10681 atgtctcttt aagccttgcc atgttcttgg gcccccttag tccaggttct tccctgtcgc
    10741 cttcgattgg cgcaccacct tgtcgccctc tttcaccctg cggatgcgga cacctttctt
    10801 cgcgtccgga tcatagggca tcagattcga cagatcgatc ggggcctcgc gctcgataaa
    10861 cccgccttcc ggcgcggtct gggaacggcg gatggccttt ttcaccatgt tcaggccctg
    10921 caccaccgct ttgcccttct tgggcatgac gcgcagcacc ttgcctgtct tgcctgcgta
    10981 atcgcctgct atggcgatca ccgtgtcctc tttcttgatt ctcgcaacac tcatgctgtt
    11041 tcctcacccg taaccgtttt ggccctgaac gggaaccgcc ccgcaggcgg accatcggcg
    11101 ttacggccgc tcgcaggctc agaccacttc cggcgccagc gaaatgattt tcatatagtt
    11161 cctgtcgcgc aactcgcgcg ccacaggccc aaaaatgcgc gatccgatcg ggttcatctt
    11221 gttgtccacg atcacgcacg cattctggtc gaaacggaca tacgagccgt cactgcggcg
    11281 gatcggctga ccggttcgca caatcacagc cgtgcagacc tgcccctttt tgatcgcgcc
    11341 cgtaggcgtc gcttccttga tcgccacgcg gatcacatcg cccaaacggg cgtagcgttt
    11401 ccgctgaccc aggacacgga aacacatggc gcgtttcgcg ccggtgttgt ccgccaccgt
    11461 caaaaccgtc tgttcctgaa tcatgtcact ttccctccgg cttcacgacc cgccagcgct
    11521 tggtcgcgct cagcggacgc gtctccatga tggtgacggc atcgccgacc tgaaccgtat
    11581 ccgactcatc gtgggcatga tactttttga accggcggat gaccttgccg tagaccggat
    11641 gccgttcgcg gcgctcgctc tgcacgatca ccgacttccg gccgctcttg ctgatcacct
    11701 tgcccttgcg gaccttgcgg cttccgcgcg tctggactgc ttcggttgtc ccggccattt
    11761 acttagcctc ccgttgggtt cgtatcgtct tcatgcgcgc gatgtcgcgc cgcatcagac
    11821 gcatccggac cggtttttcg atgccggcac ccgacttgtg tttgatccgc agatcgttca
    11881 gttcctgcga cagttcacgg atcttctggt cgagttccgc tgttccaagt tctctcagat
    11941 ctcttgcttt catgattccg ttcagactcc cgttacgtgg cgtgtgacga gcgtcgtccg
    12001 gatgccgatc ttggtgtccg ccaaacgcag gcattcccgc gccacggagt ccggcacgcc
    12061 gccgatctcg aacagcatct tgccgggcag gatcacggcg acccacagct ccggattgcc
    12121 tttgccgccg cccatgcgca cttcaatcgg cttgcgcgtc accggcttgt cgggaaacac
    12181 gcgaatccac agcttgccct tgcgtttcat ttcgcggttg atgaccacac ggcacgcctc
    12241 aatctgcgtg ttggtgagcc aaccgcgctc cagcgcctta aggccgaact cgccgtaagc
    12301 gagctgtgcg cccgaggtcg cgtgaccggc gcgatttccc ttgaattgct tgcggtgctt
    12361 cacccgctta ggcatgagag gcatctcttc tcctcctctg tccgccggcg cggaccggct
    12421 ggaaatcgtc cttcttgcaa atccagacct tgacgccgat caaccctgcg gtcgtgtacg
    12481 cctcggcgaa cccgtaatcg atgttggcgc gcagcgtgtg caacgggacc ttgccctgct
    12541 tgctccactc cacacgggag atctccgcgc cgttgatccg gccgctgcac cggattttga
    12601 tcccatcgac acccatgtcc atggcgacct tctcggcgcg cttcatcgcg cgcttgagcg
    12661 caacgcggcg ttcaacctgc tgcgcgatgc tctccgccac caattgggcg ttggcatcgg
    12721 gatcacgcac ctcatggatc tcgagataga tctctttacc cgtctttttc gcgaggtcct
    12781 ggcggattcg atcgacatcc tgccccttac gcccgatcac cacgcccgga cgggcggtga
    12841 acaggttgat ccgcacacgg ttcgcgtaac gctcgatcag gatcttcgtg atcgccgcgc
    12901 tctcgaggcg tttcttcacc aattcacgga tcatcaggtc ttcggccaac agttcgccga
    12961 actgcttttt gttggcgaac cagcggctac gccactcttt gtcaaccgcg agcctgaaac
    13021 caaccggatt tactttctgt cccaacgtct tgctcctttt gaaaagccgc cggctctcac
    13081 tgaccgtcgg tcaggaccac cttgcaatga cacagcctct tggcaatcgg gctcgcgctc
    13141 ccgcgggcgc gcggccagaa ccggcgcaga cgcgaaccct cgtcaaacac gcacaagctc
    13201 accttgagcg tgtcgacatc cagattcgcg ttgttccgcg cgttcgccac cgccgacttg
    13261 agcgtcttgc tcagaatcgt cgcggccttg cgcggactaa actcgacaat gttcagggcg
    13321 gcggccaccg gcagcccctg gcacttgcgg gccagcggcc ggcctttctt ggccgacatc
    13381 ctgacgttac gggtaatggc agtcacttcc atgcttctcg actccgttat ttggcgaccg
    13441 ctttatccgt atggaccccg tgctgcctga acgtgcgcgt cggcgcgaat tcacccagac
    13501 gatgccccac catgttttcg gtgataaaga tcggtacatg ctgtttgccg ttgtgaatgg
    13561 caaacgtgtg cccaacgaat tccggcagaa tcatcgagcg gcgcgaccag gttctgatcg
    13621 gctgcttcct gccggacgcg ttcattttct cgactttatc gagcaggctc ttctctacgt
    13681 aagggccttt cttgatagac cgtgccatgc gttactttct ccgcttcaca ataaacttgc
    13741 tgctggtctt ccggcggctg cgcgtcttgc cgcccttcgc cagcttgccc caaggcgacc
    13801 gcgggtgact gccgccggac gtacggcctt cgccgccgcc catcggatgg tcaaccgggt
    13861 tcatcgccac accgcgcacc gtcggacgga tgcccatccg acgtttacgg ccggccttcc
    13921 ccagcttgat gctgccccac tccgcattgc cgacctgtcc gatcgtcgcc atgcaacgcg
    13981 cgttgatcag acggatctcg ccggagggca tgcgcaacgt cacgaaatcg ccgtcgcggg
    14041 ccatcacctg gcacccgttc cccgcggaac gcgccagttt gccgccctgc ccgggaatca
    14101 tctcgatgtt gtgcaccgtc atccccagcg ggatttgccc caacggcatg gcgttaccca
    14161 cgttcggctc ggcttccggc cccgccatca ccgcatcgtt caccttgagc cccgccggag
    14221 ccagaatgta acgcttctcg ccgtccacat acttcagcaa ggcgagattc gcggaacggt
    14281 tcgggtcgta ggcgatcgcc tccacgcgcg ccggaatgcc gattttatta cgccggaaat
    14341 ccacaatccg gtaccggcgt ttcgcaccgc cgccgcgatg gcgcgtggtg atccgcccct
    14401 gattgttgcg cccggcctta cgcttgcgcg cctcggtcag tctccggaga ggcttgacct
    14461 cggtaatctc gtcgaacgtc ggcgacacgc ggtgccgcag tccggcgctt ctcggtttga
    14521 atgtcttgag ccccatggcc tgttatcctc tccgctacct taaacagtct cgatgcgttc
    14581 gcccgccttg accgtgacga tcgcgcgttt ccaacttgga tcacggacca cgcggcgatt
    14641 cttcagggta cgcagcgcgc ctttgtagtt ctgggtgttg accgccagaa catgtacgcc
    14701 gaactgcttc tcgaccgcct ccttgatctc gattttgttc gccttcgggg cgacctcaat
    14761 caaataccgg ttttcgcagg aaagccgtgt acccttttcc gtgatctgaa ccttgcggat
    14821 gatccctgcg tgcttcattc gtccacctct ttcttcttca ccggccgcat acgcgccgtc
    14881 agcgcctcga accccgcctg cgtggcgatc agcttgcggt ataaaagaag ggaatatacc
    14941 gtaacttccg ctgcggtgac aacctcaacc ttctgcaagt tgcgcgccga caggaccacg
    15001 ttctcatcat acgcatccag cacgatcagc accgaacggt ccgcgccgag tttcttcagc
    15061 atcgcggtca tctgcttggt cttgggtccg gtcagatcga acttttcgat taccgcgacc
    15121 cgcccctccg caatcacgtc gctgagcgcg cgcgtaaacg cgagctgctg caccttgcgg
    15181 ttgattttct gcgagtaatc acgcggcttg ggcccgaagg ccacaccacc gccgcgccac
    15241 accgggctct ggcggtatcc cgcgcgggct cgcccggtgc ccttctgcct ccacggcttc
    15301 cggttgctgc cggcgacctc gcccttgctc agcgtagagg ccgtgccggc acgggcgctg
    15361 ttgcgtatcg ccaccaccac gtccttgacc gcctgcgcgc ccttttccag ctccagccgc
    15421 tctttagcga acgtcatttc gcccgctggc tctccggccg gattaaaaac cttgatcgta
    15481 ctcatcggtt ctccaacccc tattttttca gggctttcct gatcatgacc acgccgcctt
    15541 tcggccccgg caccgaaccg gccaccagca gcaggttgtc ctcaccacgc acctgaacca
    15601 ccgtcaggtt ccgcgccttg cggttcacat tgcccatctg cccgggcatg cgcttgcctt
    15661 tgtgcaccgt tcccggaaac tcgcggcacc cgatggaacc gatgcggtgt ttcgaatgcc
    15721 cgccgtgcgt gtgcgcgccg ccgccgatgc ggtaccggcg gatcacgccc gcgaacccgc
    15781 gacccttggt cacgccgctc acgtcgacgt atttcacacc gtcaaacatc gtgaccgtga
    15841 ccatctcgcc cggcttgacc tcctcgccgt cgtccagcgc gaactcgcgg gtcagacggc
    15901 agggcttcac gcccgccttc tcgaagtgct tcacctgcgc cttggacagg cgcgagggtt
    15961 tttgctcctc gaagccgagc tgcaccgcgc ggtaaccgtc ggtctcaggc gtcttcacct
    16021 gaacgaccgg gcacgggccc gcttccaaca ccgtcaccgc aatccgacgc ccctggtcat
    16081 caaagacctg cgtcattccg atttttctgc caagtaagcc ttccatggtg tcaccgtcct
    16141 tagattcgaa ttgtgatgtc cacgccagcc ggcaggttca acttcttgag ttcatcgacg
    16201 gttttcgccg tcggccccac gatgtccagc agccgtttgt gtgtgcgcat ctcaaactgg
    16261 tccatggact tcttgtccac atgcgggctc cggttgaccg tgaaacgctc aatcctcgtc
    16321 ggcagaggaa tgggtcccac gacttgagcc ccggtgccgc gggccgtgtc gatgatgtcg
    16381 ctcaccgcct gatccagcac cctgtgatcg tacgcttgca aacgaatgcg tatacg
//
LOCUS       DAOSRB010000045        16259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3682_length_16259_cov_8.223216, whole genome shotgun sequence.
ACCESSION   DAOSRB010000045 DAOSRB010000000
VERSION     DAOSRB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16259
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3682_length_16259_cov_8.223216"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(361..2214)
                     /locus_tag="PLZ74_04015"
     CDS             complement(361..2214)
                     /locus_tag="PLZ74_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOR97519.1"
                     /translation="MTAERQSMEADIVCVGFGPATAGFLTTLTRAMMKPDGTPAFESK
                     VMPGMPLQVMCYERADDIGFGVSGIVTKGRHISASFPDLDLAKEIPNAAEISREEVAY
                     LFDTVGASRRSAGTKFMDKVFRYGQFAMRKAPSATELPWIPPFLRKEPGLVMSMGSFL
                     SWAGNQLMGSGLVQIWPGTPVSAPIMEGDCVAGVRLTDQGVDKKGNPEAAYMPGMDIR
                     ARLTVVGDGPVGAVGRKLDRHFGLPEGHHQHDWAVGMKAVVELPASCDLKPGTVLHTL
                     GFPEPEIFGFLYVYPDRTASLGIFVPSWMDNPIRTTYRYLQHWMQHPYLWRYLQGGTL
                     RSWGAKSLQESGVRGEPYLVGDGFARIGEGSGTTNVLTNSGVDEAWRSGVLLAEGVID
                     LLARDQPFTKATLTGAYVRRRRQDPLNAELQAAKKARDGFGKGFMLGMMGTGMTGMTG
                     GMLNLSVDSVPPSQRIPTLESVYGDRIAPEELSEAVKQCAKAKQPLHDTIMTRVGWPE
                     IPFDGTLLMSHQDALFKGGKVQAAGGFADHVTFADPALCVSCNERTCIEICSAQALMP
                     GEEGGTPEFDREKCVHCGACIWSCSKALPQDPERANIAFGAGSGGLHSNEN"
     gene            complement(2625..4166)
                     /locus_tag="PLZ74_04020"
     CDS             complement(2625..4166)
                     /locus_tag="PLZ74_04020"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR97520.1"
                     /translation="MKRIWTSGARKVASLLALTLALTARAGVPVDPNLVALFADTHAT
                     ADAKRTNQRAGVERCVREILALNPRPANLLILGDLSFDHGVTNDYQTLRDLLSPLAKT
                     GVRWHACFGNHDRRASFFAVFPEQRAAHPPVPERHVSRVETPYADFILLDSCLEGPVN
                     GGLDDAQRAWLEKTVAANRKPLFLCAHHGIGETGVAGLMVSNALCKAYLFGHHHNAAH
                     REEKGVRTFCLPSTGHWGDIGFMLMRLTPEEARLTLRQHDYYTRPPTRPEDVNPEWRE
                     RTIRKNGLEWRVPLATAGGFDTLFNGTDFQGWRRPQGDNNHWRILDGVIDYDARSEAK
                     GDKNLWSERSLRDFDLHLEWRIKETPYVNPHARRILPDGSEAAGPDGKPLGLAIPDSD
                     SGVYLRGSGKHQVNIWCWPVGSGEMYGVRRDSAMPPEVRAGVTPRVCADRPVGEWNRF
                     EITVRGNTVRVLLNGQAVLPGVTIPGLPAEGPIALQHHGSLRDGKWVSPPSLVQFRNI
                     FVREL"
     gene            complement(4371..4553)
                     /locus_tag="PLZ74_04025"
     CDS             complement(4371..4553)
                     /locus_tag="PLZ74_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97521.1"
                     /translation="MKIRNSPWRSKKTRAVPSRVFCGETIDPDSDPDPDPDLDETMKP
                     ETLKPKRVEGSTALFG"
     gene            complement(4697..5662)
                     /locus_tag="PLZ74_04030"
     CDS             complement(4697..5662)
                     /locus_tag="PLZ74_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOR97522.1"
                     /translation="MTQPISNLRFEYMNTSERRVLNLEPLGLPNVPVLGYCHYRSPRP
                     DVPEHWHPGCVEIHLGVRNVVEFGFDGRTYRLGPGDVFVNLPGERHTVSAHPNGLILY
                     WLILRVGRSRIPFLGQPETEAEALRKSLLQLPHRHFRGTETLKRLFRSLHLLCDEPAT
                     HLRALRLRTTLLALLLEVVNAAEVHRDSPGETRLEALLREMRTAPEKAYRLDDLARRA
                     GMAPSHFIARFRSLTGLPPHHFLLHTRIEAARRLLRESDVPITELALRLGFCSSQHFA
                     HIFKRHTGITPRACRKGACGPLRAGDDNDGQSGEKRKEQANALMS"
     gene            5841..7211
                     /locus_tag="PLZ74_04035"
     CDS             5841..7211
                     /locus_tag="PLZ74_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOR97523.1"
                     /translation="MFTRGEPKKATLKRDFKPPITLLRELQRAARPGGKTLAIAWERE
                     NGYVSRYDLPLPPRAEDSPEVAQLAERCVKFLLWAAGGWRLMLSGPQKLCQSIKKQYS
                     ARGARAFDVAFMEKVYDRPMVVELRKPSEMPQAHERALVMDKRTNGCRIGFDLGASDF
                     KISAVKNGKAIYSDEFPWDPRNQTDPDYHYNKLNEGLKIAAAKLPRVDAIGGSSAGVI
                     VDNRIRVASLLRAIPEARYAEAQNMFVRLAEEWKVPIEVANDGDVTALAGLMSLGKKG
                     ILGVAMGSSEAVGFIDRNGCLTGRLSELAFAPVDLHPEAPADEWSGDIGVGAMYFSQQ
                     AVNHLAVKKGMRFPAKMALPQRLERVQERMAEGDVTALKIYQKIGLYLGYTVPWYREF
                     YGFDSMMILGRVTSGAGGVMILETARLILADQFPELAEEVEVFMPDEKARRVGQSVAA
                     ATLPLI"
     gene            7301..8143
                     /locus_tag="PLZ74_04040"
     CDS             7301..8143
                     /locus_tag="PLZ74_04040"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOR97524.1"
                     /translation="MKLTHPTADLYVPDGLAIGPALARTTHLGIGAHQDDLEFMTIHG
                     ILSCFGRSDRSFCGVTCTDGAGSSRSGVYGDYTDEQMKAVRRQEQRAAAMVGRYGAML
                     QLDFTSKEVKARDNSALVNDLAVILDAARPGTVYTHAPSDKHDTHVGVTRAVIAAIRS
                     LPADARPATVYGCEIWRDLDWLPDARKVVLDVSERGNIAAALSGVFDSQISGGKRYDD
                     AVLGRRRAHATFFESHAVDEATMLTFAMDLTPLVRDDTLSLADYTAGLIDEMKADVVR
                     RIGG"
     gene            8164..9288
                     /locus_tag="PLZ74_04045"
     CDS             8164..9288
                     /locus_tag="PLZ74_04045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOR97525.1"
                     /translation="MAQAKHTREQVREVMESFPVWGDFRDAASYGSGHINDTYRVSVC
                     LAGTPVHYLLQRINHDIFRRPDQVMENIVRVTEHLYGRLTAAGESDVTRRTLTVVPAR
                     DGQPFTRDAAGNWWRLYLFIEQARTYDVIETVDQAREAARAFARFQNRLADLPAPRLH
                     ETIPAFHNTLVRLEALDHAVTEDVRGRRKDVADELAFIDARREACGRLLERHARGEIP
                     ERITHNDTKINNVMLDDATGEGVCVIDLDTVMPGLALYDFGDMVRSATAAAREDERDL
                     SQVGSRADMFEALARGYLAEAAFLNEAELEELVFSGPLITLTIGIRFLTDYLSGDVYF
                     KTHRPGQNLDRCRTQLHMVRCMEDQTAERERLVREITRAK"
     gene            9494..10009
                     /locus_tag="PLZ74_04050"
     CDS             9494..10009
                     /locus_tag="PLZ74_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97526.1"
                     /translation="MDLSEQQRQAVKEWLAEGASLSDVQKRLKSEFDLMLTYMDVRLL
                     ILDIGATVQDKPEPKPAEPAAESRVPPPEGEDPYDDVFDDEPGGGLPSGEDGAAGGKV
                     SLTLDRLVVPGAMFSGDVTFSDGVKARWMIDQIGRFGLDPEKPGYRPSDADLQAFQLQ
                     LRSELQRHGYA"
     gene            10074..10877
                     /gene="proC"
                     /locus_tag="PLZ74_04055"
     CDS             10074..10877
                     /gene="proC"
                     /locus_tag="PLZ74_04055"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOR97527.1"
                     /translation="MKIGFLGAGAMAEAILASVLRNGLCKPGDVLACDKDEGRRVLMK
                     ERYGVRVTDDPAEVVRRYAVLLLAVKPQDLDALLSTLKPLLKARHLLISIAAGKTLAA
                     LKKAAGPHPRRIRVMPNLPLSVQEGMSVYCAAAPAKLADRRLVARLFGSAGAVLELPE
                     RQFDAVTALSGSGPAFVAFVAQALIAGAQALGLPPDAARLLTEQTLIGTGVYLQNTGR
                     DLDEFIRAVASPRGTTAAGLAVLRKSAVPATLARTLAAAAARSAELSRA"
     gene            10910..11161
                     /locus_tag="PLZ74_04060"
     CDS             10910..11161
                     /locus_tag="PLZ74_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOR97528.1"
                     /translation="MEARIGFVGILLEHRDSVPEVNRILSDHADRILARVGLPCRDRR
                     VSVITLVVEATTDQLGDLTGRLGQVAGVSVKSALSKERA"
     gene            11158..12054
                     /locus_tag="PLZ74_04065"
     CDS             11158..12054
                     /locus_tag="PLZ74_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOR97529.1"
                     /translation="MSRWLSVSQHGRCLLAVLLSLRFLGGARGADRPLVLVSIPPQAW
                     LVKRLADGAVAVESLLADAANPHLFEPDARQARLLAEASIYFTLGLPFERAVLSRAAR
                     MPTRPAFHAMDAGIAKHGARHGHAAPGHACSGTEDGDPHIWLSPVLMCAMASNTVAAL
                     SERFPAHGDAFGAALGRTVAEICALDAEMRERLRPLATRTFAVYHPSWDYFAETYDLV
                     SLVLEREGKAPSARHLADLIRQARAAGVRVVFADPQYDPRPVHALASQLGARVVSLNP
                     LQETWPVLLRRAAAALADEETP"
     gene            12051..12836
                     /locus_tag="PLZ74_04070"
     CDS             12051..12836
                     /locus_tag="PLZ74_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOR97530.1"
                     /translation="MTAPVIDVTDVCFGYGSEEVLHNVSFAVLANTLVAVVGPNGGGK
                     TTLLKLLLGELVPRYGSIRVLGGAPAETRTRVGYVPQSLAFDPLVPVSAREVVLMGRV
                     GRARFGLYGGSDRRRADDALARVGLEDFGRRAFAELSGGERQRVLIAQALAGGSELLL
                     LDEPVANVDPEQTERLYALFQELTATVTVLMVSHNLSVVTDRATHVLCVNGTAELHAI
                     NEVASSTFAAAYGGRLTTIRHDASCHVLDASGVMHRPHHGEEG"
     gene            12874..13707
                     /locus_tag="PLZ74_04075"
     CDS             12874..13707
                     /locus_tag="PLZ74_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOR97531.1"
                     /translation="MMLFFEDFLRFPFLRHAVLAVVLASVAAGLVGSLVVVRRATYLA
                     GAVSHSLLAGLGLARLLQRRFGIGFLTPMGGAVLTALAVVLLVTHLTARGRHRTDTVL
                     SAVWTGGMALGVSCVAATPGYQDDLMSYLFGSLLLVTGTDLFWMAVLDAVLVAVILLC
                     HNRFLAIAFNEELARLRGVNTALYELLFHIMTALTVVLLVRVVGIVLAIALLTLPAAA
                     AGRLTRSLDRMMLLAAGLCLLVSLIGLVVSYAPGWPPGATIVELAVLVYLGTSLTRAR
                     R"
     gene            13812..15083
                     /locus_tag="PLZ74_04080"
     CDS             13812..15083
                     /locus_tag="PLZ74_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOR97532.1"
                     /translation="MATDLKAAYKTIMDDHFTPQMEITFLDGGKRQTLCYEKVSWVID
                     GEEKGLRYGENPGQEAALYKLVNGNLTLGEVTALTPGNVLASEPELLQSGKHPGKTNL
                     TDADNALNILRYLDATPCAVIVKHNNPCGVAKGPTLAEAYFRANKADRLAAFGGCIAL
                     NREVDIETARLVAENYAEVVVAPEFAPGVMDLFATKKNLRVMRIRNMARLTSFVARRV
                     VDFKSLIDGGLVAQWSFVPEARTPEQLLPAECTTKDGTVHRIRRLPTPQEYEDLIFGW
                     LVEAGVTSNSVLYVKDGATVGIGTGEQDRVGVAEIARDKAYRKLADWIAWEKYATPYN
                     ELVLKFGDLGKSRMGEIDEEVKARKGGLPGCSMVSDAFFPFRDGAEVGIREGITSIVQ
                     PGGALRDWDVIDCCNEAGVAMVFTGQRSFKH"
     gene            complement(15098..15793)
                     /locus_tag="PLZ74_04085"
     CDS             complement(15098..15793)
                     /locus_tag="PLZ74_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97533.1"
                     /translation="MQTNPWIIGLKAARANLVPGLILQAAAFALLASYTFLPPVRHAL
                     TEVGRWQARFGVPFSLASYLFFCGVVPYLFCLACPALRPAQRGRALAFALGFWGLMGL
                     LIPQFYRLQSALYGNGTDVQTLVCKILTDQFVFSAFLTVPLVSLAHFWKDCGYRWEAV
                     APHMGRGWYSRLVLPNLVMNWTVWIPSLCVIYSLPTALQPHVAGLIGGFWALMCLQIA
                     AHTPKTTRQPDCG"
BASE COUNT         2912 a         4872 c         5421 g         3054 t
ORIGIN      
        1 atcgattccg atagcgatcc cgaccccgac cccgatttgg atgaaaccat gaaacacata
       61 aaacttcatg aaaatcggga attccccctg gcgttccaaa aaaacccgag cgatgccatc
      121 ccgtgtgttc tgtggtgaaa caatcgattc cgatagcgat cccgatcccg accccgaccc
      181 cgatttggat gaaaccctga aaccctgaac ccttgaaacc agaaaccaga aaccctgaaa
      241 ccaaagagag ttgaagggtc aactgttctt tttagattca acatccctct tccggtcatc
      301 cgtctgatcc aaaaggtacg caagcggatt tcttttccgg cgggtgtctc cgcgctacgg
      361 tcagttctcg ttcgaatgca gtcctccgga gccggcgccg aacgcgatgt tggcacgctc
      421 ggggtcttgg ggcagagctt tggagcaact ccagatgcag gcgccgcagt ggacgcactt
      481 ctcgcggtcg aactcgggtg tgccgccttc ctcgccgggc atcagggcct gagccgaaca
      541 gatctcgatg caggtgcgct cattgcagga aacgcaaagc gcggggtcgg caaaggtcac
      601 atgatcggcg aacccgcctg cagcctgcac ttttccaccc ttgaagagcg cgtcctgatg
      661 cgacatcagc aacgtgccgt caaaggggat ctcgggccag ccgacccgtg tcatgatcgt
      721 gtcgtgcagg ggttgcttgg ctttggcgca ctgtttgacc gcttcgctga gttcttcagg
      781 cgcgatccgg tcgccataga cactctccag cgtggggatg cgttgcgagg gggggacgga
      841 atcgaccgaa aggttgagca tgccaccggt catgccggtc atgccggtac ccatcatccc
      901 gagcatgaag cccttgccga agccgtcgcg cgcctttttg gcggcctgca attcggcatt
      961 gagcggatcc tgacggcggc ggcggacgta cgcgccggtc agcgtggcct tggtgaaggg
     1021 ttggtcgcgc gcgagcaggt cgatcacgcc ttcggccaac aagacaccgc tacgccacgc
     1081 ctcgtccaca ccggagttgg tcagcacgtt cgtcgtgccg gaaccctcgc cgatgcgggc
     1141 gaacccgtcg ccaaccagat agggttcacc gcgcacgccg gactcttgca gcgactttgc
     1201 gccccatgag cgcagtgtgc cgccctgaag gtaacgccac aggtaagggt gttgcatcca
     1261 atgctgcagg taccggtatg tggtgcggat cggattgtcc atccacgagg gcacgaaaat
     1321 tccgagtgag gcggtgcggt cgggatagac gtacaggaaa ccgaaaatct cgggttccgg
     1381 gaagcccagc gtgtggagaa ccgtgccggg cttgagatcg cacgaggcgg gcaactcgac
     1441 cacggctttc atgcccacgg cccagtcgtg ctggtggtgg ccttcgggga gcccgaagtg
     1501 gcgatccagc ttgcggccga ccgcgccgac cggtccgtcc ccaaccacgg tcaggcgcgc
     1561 gcggatgtcc atgcccggca tgtaggcggc ttcggggtta cccttcttgt ccacaccctg
     1621 atccgtcagg cgcacacccg ccacgcaatc gccctccatg attggcgcgc tcacgggggt
     1681 gcccggccag atctggacaa ggccggagcc catgagctgg ttgccggccc atgacagaaa
     1741 cgagcccatc gacatcacga gacccggctc tttgcgcagg aagggcggga tccacggcag
     1801 ttcggtagct gaaggcgctt tgcgcatggc gaactggccg tatcggaaga ccttgtccat
     1861 gaacttcgtg cccgcactgc ggcggctggc acccacggta tcgaagagat aggcgacctc
     1921 ctcgcggctg atctctgccg cgttgggaat ctccttcgcc agatcgagat cggggaacga
     1981 ggcgctgatg tggcggcctt tggtgacgat gccggagaca ccgaacccga tatcgtcggc
     2041 gcgctcgtag cacatcacct gcaacggcat gcccggcatc accttgctct caaaagcggg
     2101 tgtgccgtca ggcttcatca tggcgcgggt cagagtggtc agaaatccgg cggtggccgg
     2161 cccgaaaccc acacacacga tatcggcttc catcgattgt ctctctgctg tcatgatctg
     2221 tccttgtctg tagcttggtc gttttgtcac gccctcgccc catgtttttt ctgcccgtca
     2281 tgttacaaag cgtgcagttt cctgtcaacg agacgcatgg gatcgatatc gctatctgaa
     2341 tcgttgtcgg gttcgaccgc cttgtctata acttagccat tttgccacac cttcgggcgt
     2401 ccgagacgtc cgtccgcact ttacagtgct cctccggtcc gtgcccgtac ggcgggacgt
     2461 ccacagctac gtgccattgg cgcggagccg gaccgccgaa cccgctgcct cgtcaaacaa
     2521 ccaaaacgtc gcaggtcgaa tgccgtgggt cgcaagtcaa cgtacacgcg aacgagaacg
     2581 gggaagcaca ccgatcccga tgcctctggc cgaagcgggc ttgtctacag ctcgcggacg
     2641 aagatgtttc ggaactggac gagggagggc gggctgaccc acttgccgtc gcggagggaa
     2701 ccgtggtgct gcagggcgat cgggccttca gcgggcagac cgggtatcgt cacgccgggt
     2761 agcacggcct ggccgttgag gagcacgcgc acggtattgc cgcgcacggt gatttcgaag
     2821 cggttccact cgccgaccgg gcggtcggcg caaacccggg gcgtgacccc ggcgcgcact
     2881 tcgggcggca tggcggagtc gcgccgcaca ccgtacatct cgcccgagcc cacgggccag
     2941 caccagatgt tgacctgatg cttgccggag ccgcgcagat agacgccgga atcagaatcc
     3001 gggatggcga ggcccaacgg tttgccgtcc ggacccgcgg cctcgctgcc gtcgggcagg
     3061 atccggcggg cgtgggggtt gacgtagggg gtctctttga tgcgccattc gaggtgcaaa
     3121 tcgaaatcgc gcagagacct ttcgctccag agattcttgt cgcctttggc ctcactgcgg
     3181 gcatcgtaat caatcacgcc gtcgaggata cgccagtggt tgttatcgcc ttgggggcgg
     3241 cgccagcctt ggaagtcggt accgttgaag agtgtgtcga aaccgccggc ggtggcgagc
     3301 ggcacgcgcc attcgaggcc gttcttgcgg atcgtgcgtt cgcgccattc ggggttgacg
     3361 tcctcaggcc gagttggggg ccgggtgtag tagtcgtgtt ggcggagggt gaggcgcgcc
     3421 tcctccgggg tgagacgcat cagcatgaaa ccgatgtcac cccagtgtcc ggtggaagga
     3481 aggcagaagg tgcggacccc tttttcctcg cggtgtgcgg cgttgtggtg gtggccgaag
     3541 aggtaggctt tgcagagggc gttggaaacc atgagaccgg ccacgccggt ttcaccgatg
     3601 ccgtggtgcg cgcagaggaa gagcggcttg cggtttgcgg ccacggtttt ttcgagccag
     3661 gcgcgttggg cgtcgtccag accgccgttg accggaccct cgagacagga atcgagcagg
     3721 ataaagtccg cgtacggggt ctcaacccgt gaaacgtggc gttccggcac gggggggtgt
     3781 gcggcgcgct gttccggaaa gaccgcgaaa aatgacgcgc ggcggtcatg attcccgaag
     3841 caggcgtgcc agcgcacacc ggtcttggcg agggggctca gcaggtcacg cagggtttga
     3901 taatcattcg tgacgccgtg gtcaaacgag aggtcaccga ggatcagcag gttcgcgggc
     3961 cgggggttga gggccagaat ctcgcggacg cagcgctcca caccagcacg ctggttcgtc
     4021 cgcttggcgt ccgccgtggc gtgcgtgtcc gcgaagagcg ccacaaggtt cgggtcaacg
     4081 ggcacgcccg cccgggcggt gagggccaga gtcagagcga gaagcgacgc aaccttccgt
     4141 gcgccggatg tccaaatgcg tttcatgtca tttccccttg tgctgatgag aatgccgcgc
     4201 acacccttgc gcgacaaccg ggaaaagcat gacatagatc gcgcagaaag ggaatcggaa
     4261 tcgaatggcg ttggttcatt agttcattag tgcgttagtg cgttagtgca ttagtgcgtt
     4321 agtgcattat tagtcgggat cgctatcgct atcggaatcg ggatcgatgt ttaaccaaaa
     4381 agagcagttg aaccttcaac tctctttggt ttcaaggttt ctggtttcat ggtttcatcc
     4441 aaatcggggt cgggatcggg atcgctatcg ggatcgattg tttcaccaca gaacacacgg
     4501 gatggcactg ctcgggtttt tttggaacgc cagggggaat tcctgatttt catgaagttt
     4561 catgtatttc atggtttcat ccaaatcggg gtcgggatcg ggatcgctat cgggatcgct
     4621 atcgggatcg attgtttaac cgtagcccca cagtcccaca gcctttggga ttgcgggctc
     4681 cgcccgcatt agttcattag ctcattagtg cattcgcttg ctccttccgt ttttctccgc
     4741 tttggccgtc gttgtcgtca cccgcgcgaa gcgggccgca ggcaccttta cggcacgcgc
     4801 ggggcgtgat gccggtgtgg cgcttgaaga tgtgggcgaa gtgctgggac gagcagaacc
     4861 cgaggcgcag ggcgagttcg gtgatcggca cgtcgctctc gcgcagcagg cggcgggccg
     4921 cctcgatgcg ggtgtgaagc aggaagtggt gtggcggcag gccggtcagg ctgcggaagc
     4981 gcgcgatgaa gtgcgagggg gccatgcccg cgcggcgggc gaggtcatcc agccggtatg
     5041 ccttctcggg cgcggtccgc atttcgcgca gcagggcctc cagccgcgtc tcgcccgggg
     5101 agtcgcggtg gacctctgcc gcattcacca cctccagcag cagcgccagc agggtggtgc
     5161 gcagccgcag ggcgcgcaag tgcgtggccg gttcatcgca gagcaggtgt agcgaacgga
     5221 aaagccgctt cagggtctcg gtgccgcgga agtgacgatg ggggagttgg agcagcgatt
     5281 tccgcaacgc ttcggcttca gtctccggct ggcccagaaa cggaatgcgg gatcgtccca
     5341 cgcgcaggat cagccagtac aggatcaggc cgttgggatg cgcgctgacc gtgtggcgct
     5401 cgccgggcag gttcacgaac acgtcgccgg gaccgaggcg gtaggtacgg ccgtcaaagc
     5461 cgaactccac cacgttgcgc actccgagat ggatctcgac gcaaccggga tgccagtgct
     5521 ccggcacgtc agggcgcggg ctgcggtagt ggcagtaccc cagcaccggc acgttcggca
     5581 gacccagggg ctccagattc aacaccctcc gctcggaggt gttcatgtac tcgaaacgca
     5641 gattggagat gggttgcgtc atgtgcgtaa tcttatgaaa cgaaaaagat gaacgcaagc
     5701 ggaagagaac atcgcctatt ctgcggccgg aaagaacgtg tgggcgggtt cgcgcatgcg
     5761 tgcggcggca ggcggaagtg tccgcaaagg ctgacgcact tttcaacgcg cggcccccgt
     5821 aacccagaag gaggctgacg atgttcaccc gcggcgaacc gaagaaagcg acgttgaaac
     5881 gcgatttcaa accgcccatc acactactgc gagagttgca gcgcgcggcg cggccgggcg
     5941 gaaagacgct ggccatcgct tgggagcgcg agaacggcta cgtttcgcgt tacgatctgc
     6001 cgttgccgcc gcgcgccgag gattcgccgg aggtggcgca gctcgcggaa cggtgcgtga
     6061 agtttctgct ctgggccgcc ggcggctggc gtctgatgct ctccggtccg cagaagctgt
     6121 gccagtcgat caaaaagcag tactcggcgc gcggcgcgcg ggcgtttgat gtggccttca
     6181 tggaaaaggt ctatgaccgt ccgatggtgg tggaactgcg caagccgagc gagatgccgc
     6241 aggcgcatga gcgtgcgctc gtgatggaca agcgcaccaa cggctgccga atcggcttcg
     6301 atctgggggc gagcgatttc aagatcagcg cggtcaagaa tggcaaggcg atctacagcg
     6361 atgagttccc gtgggatccg cgcaaccaga ccgatccgga ttaccactat aacaagttga
     6421 acgagggatt gaagattgcc gcagccaagc tgccgcgggt ggacgcgatc ggcggtagca
     6481 gcgccggcgt gatcgtggac aatcggattc gggtggcttc gctcctgcgt gcgattccgg
     6541 aagcgcgtta tgctgaggcg cagaacatgt tcgtccgtct ggcggaggag tggaaggtgc
     6601 cgatcgaagt cgccaacgat ggcgacgtga cggcgctggc cggtctgatg tcgctcggca
     6661 agaaagggat tctgggcgtg gccatgggat caagcgaggc cgtcggtttt atcgaccgca
     6721 acgggtgcct gaccgggcgt ctctcggaac tcgctttcgc gccggttgat ctgcacccgg
     6781 aggctccggc cgacgagtgg tcgggggata tcggtgtcgg cgcgatgtac ttctcgcagc
     6841 aggcggtgaa ccatctggcg gtgaagaagg gcatgcgttt cccggccaag atggcgctgc
     6901 cccaacgctt ggagcgcgtg caggagcgta tggcggaagg ggacgtgacg gctctgaaga
     6961 tctatcagaa gatcggcctc taccttggct acaccgtgcc gtggtaccgt gagttttacg
     7021 gctttgacag catgatgatc cttgggcgcg tgacgtccgg cgcgggcggg gtcatgattc
     7081 tggaaacggc acggctgatt ctggccgacc agtttcccga acttgcggag gaggtcgagg
     7141 tgttcatgcc cgacgagaag gcgcggcgcg tgggccagtc tgtggcggcc gcgacccttc
     7201 cgctgattta gcgggagcgt gcggcgcgga tcgtttttta ctctcgtctc cgtgtccccc
     7261 cccccgtgat cacggcaacc gaaaatagga gtaacgcaat atgaaactga ctcatccaac
     7321 agcggatctg tatgtgccgg acggtctggc gatcggtccg gctctggccc gcaccacgca
     7381 tctcgggatc ggcgcgcatc aggacgacct cgagttcatg accatccacg gcatcctgag
     7441 ttgcttcggc cggagcgacc gctccttctg cggcgtgacc tgcacggacg gcgcgggcag
     7501 ctcgcgctcc ggtgtttacg gcgactacac ggatgagcag atgaaggccg tgcgccgcca
     7561 ggaacagcgt gccgcggcga tggtgggccg gtacggcgcg atgctgcaac tggacttcac
     7621 cagcaaagag gtgaaggcgc gcgacaattc cgcgctggtc aacgatctgg ccgtgattct
     7681 cgatgcggcc cggcccggga cggtgtacac gcatgcgccc tccgacaagc acgataccca
     7741 tgtgggcgtg acgcgcgcgg tgatcgcggc gatccgcagc ctgcccgctg acgcgcggcc
     7801 cgccaccgtg tacgggtgcg aaatctggcg tgacctcgac tggttgccgg acgcccggaa
     7861 ggtggtgctg gacgtgagtg agcggggcaa catcgctgcc gccctctccg gtgtgttcga
     7921 ttcgcaaatc tccggcggca aacggtatga cgacgccgtg cttggccggc gccgggcaca
     7981 cgccaccttt ttcgagtcgc acgcggtgga cgaggctacc atgctgacct tcgcgatgga
     8041 cctcacaccg ttggtccggg acgacaccct ttcgctcgcg gattacacgg ccggactgat
     8101 cgatgagatg aaggcggatg tggtgcggcg catcggcggc tgatcgtcgg ccggggaggg
     8161 ggtatggcac aggcgaaaca tacgcgtgaa caggtacggg aggtgatgga gtcctttccg
     8221 gtgtggggcg atttccgcga tgccgcgtcc tacggctcgg gccacatcaa cgacacctat
     8281 cgggtttctg tctgcctggc cggcacgccg gtgcattacc tgctgcaacg aatcaaccac
     8341 gatatcttcc gacggcccga tcaggtgatg gagaatatcg tgcgcgtgac cgagcacctg
     8401 tacggtcggc tgaccgccgc cggggagagc gacgtgacgc gccggaccct caccgtggtc
     8461 ccggcacgcg atggccagcc cttcacgcgc gacgcggcgg gcaactggtg gcggctctac
     8521 ctcttcatcg aacaggcgcg cacctatgat gtgatcgaga ctgtggatca ggcgcgtgag
     8581 gcggcgcgcg cgttcgcacg tttccaaaac cggcttgccg acctgcccgc gccgcgtctg
     8641 cacgagacca tccccgcgtt ccacaatacc ctcgtgcggc tcgaagcgct ggatcacgcc
     8701 gtgacggagg atgtgcgcgg ccgcaggaaa gacgtggctg acgaactcgc cttcattgac
     8761 gcgcgccgcg aggcgtgcgg tcggctgctg gaacgccacg cccgtggcga gattccggag
     8821 cggatcacgc acaacgacac caagatcaac aacgtcatgc tggatgacgc cacgggcgag
     8881 ggggtctgcg tgatcgacct cgataccgtg atgccgggtc tcgcgctcta tgatttcggc
     8941 gatatggtgc gctctgccac tgctgcggcg cgcgaggatg agcgtgatct ctcgcaggtc
     9001 ggcagccgtg ccgacatgtt cgaggccctg gcgcgcggct atctggcgga ggccgcgttc
     9061 ctcaacgagg ccgaacttga ggagcttgtc ttctccggcc cgttgatcac cttgacgatc
     9121 ggcatccggt tcctgaccga ctacctcagc ggtgacgtgt atttcaaaac ccaccgcccc
     9181 ggacagaatc tggaccggtg ccgcacacaa ctccacatgg tgcgctgcat ggaggatcag
     9241 acggcggagc gcgagaggct ggtacgggag atcacgcgcg cgaagtgatc gccgttcggg
     9301 tttgtcgcta tcgggatcgt ttccccctct cgttcgcgtg tacgccgacc tgcgaccggg
     9361 acacctccga tccgacggat cagtcggatc cgagagatcc gcatgcgccc cgaaatcaaa
     9421 tgcgtctttt ccgatgcaac cttgacgttt gcacacaggc ttgggacaat cactggaaaa
     9481 ggagatggca atcatggatt tatccgagca acagcgtcag gccgtcaaag agtggctggc
     9541 tgaaggggcc tcgctctccg atgtgcagaa acggttgaag agcgagttcg acctcatgct
     9601 gacctatatg gacgtccggc ttctcatcct tgacatcggc gcgacggttc aagacaaacc
     9661 cgaacccaaa ccggccgaac cggctgcgga gtcacgtgtg ccgccgcccg aaggcgagga
     9721 tccgtacgac gacgtattcg acgacgaacc cggcggcggg cttccgtccg gcgaggacgg
     9781 cgcggcgggc ggcaaggtga gcctgacgct cgaccgtctg gtggttcccg gtgccatgtt
     9841 cagcggcgat gtgacctttt cggacggcgt gaaggcccgc tggatgatcg atcagatagg
     9901 ccgtttcggt ctggatccgg aaaaaccggg ctaccggcct tcggatgcgg atctgcaagc
     9961 gttccagctt cagttgcgat cggaactgca gcgtcacggt tacgcgtagt gcctgacgaa
    10021 ccgatcacgg caaagaagca tgcgttgttg agcttgattt ttgggagaca gggatgaaaa
    10081 tcggttttct tggtgcgggc gctatggcgg aggcgattct cgcctcggtg ttgcgcaacg
    10141 gtctgtgcaa acccggcgac gtgttggcgt gcgacaaaga tgagggccgg cgcgttttga
    10201 tgaaagagcg ttacggtgtg cgtgtcaccg atgacccggc tgaggtcgtg cggcgttacg
    10261 cggtgttgtt gctggcggtg aagccgcagg atctcgatgc cctgttgagc acactcaaac
    10321 cgttgctgaa ggcgcggcac ctgctcattt cgatcgcggc gggcaagact ctggcggcac
    10381 tgaaaaaagc ggccggaccc cacccgcggc ggatccgcgt gatgcctaac ctgccgctga
    10441 gcgtgcagga gggcatgagc gtctattgtg ccgcggcccc cgccaaacta gcggaccgac
    10501 gcctcgtggc ccggctcttc ggcagcgcgg gcgcggtgct tgaacttccg gagcggcagt
    10561 tcgacgccgt cacagccctg agcggttcgg gaccggcgtt cgtggccttt gtggctcagg
    10621 ccctgatcgc gggcgcacag gctttgggtc tgccccctga cgccgcgcgc ctgctgaccg
    10681 agcagacgct gatcggcacc ggagtctatc tgcagaatac cggccgcgac ctcgatgagt
    10741 tcatccgcgc agtcgcctcg ccccgcggca ccaccgccgc aggtctggcg gtgctgcgaa
    10801 aatccgcggt gcccgccacc ttggcccgga ctctggccgc ggctgcggcg cgtagcgcgg
    10861 aactcagccg ggcctgagac ggcacggatc ggaagggaaa ggggaggaaa tggaagcgcg
    10921 catcggtttt gtcggcatcc tgctcgaaca ccgcgacagc gtgccggaag tcaaccgcat
    10981 cttgtcggat catgcggacc ggattctcgc acgtgtgggc ttaccttgcc gcgacaggcg
    11041 ggtttccgtg atcacgctgg tggtcgaggc caccacggac cagttgggcg acctcacggg
    11101 acgactgggg caagtcgccg gggtttcggt caagtccgcc ctctccaagg agcgtgcatg
    11161 agccgttggc tctccgtttc ccaacatggc cggtgcctcc tcgcggtgtt gctgtcgcta
    11221 cggtttctgg gcggcgcacg cggcgcggac cggccattgg tgctggtctc cattccgccg
    11281 caggcgtggc tggtgaaacg gctggccgac ggggctgtcg cggtcgagtc gttgctggcc
    11341 gatgcggcga atccgcatct cttcgaaccc gatgcgcggc aggcgcgtct ccttgcggag
    11401 gcttcgattt atttcacgct tggcctgccg ttcgagcggg cggtgctctc ccgcgcggcc
    11461 cgcatgccca cgcggcccgc gttccacgcc atggacgcgg gaatcgcgaa gcacggcgcg
    11521 cgtcacggcc acgccgcgcc aggtcatgcc tgctccggca cggaggacgg ggatccgcac
    11581 atctggctca gtccggtcct gatgtgcgcg atggcctcca atacggttgc cgccctcagc
    11641 gaacgctttc cggcgcacgg tgacgcgttc ggagcggccc tcgggcgcac cgtggcggag
    11701 atttgtgcat tggacgcgga gatgcgcgag cgcctgcgtc cgctcgcgac gcgcacattc
    11761 gcagtgtatc acccctcctg ggactatttc gccgaaacct acgacctcgt ttcgctggtg
    11821 ctggagcggg aaggcaaggc tccgtcggcc cggcaccttg cggatctgat ccggcaggcg
    11881 cgcgccgccg gggtccgggt cgtcttcgcc gatccgcagt acgatccgcg gcccgtgcac
    11941 gcgctggcca gccaactggg cgcgcgcgtg gtttcgctga atccgctgca ggagacgtgg
    12001 cccgtgctgt tgcgtcgtgc ggccgcggct ctggccgatg aggagacccc atgaccgcgc
    12061 cggtgatcga cgtgacggat gtttgtttcg gctatggcag cgaggaggtg ctgcacaatg
    12121 tctccttcgc ggtcttggcg aacaccctgg tcgcggtggt cggccccaac ggcggcggga
    12181 aaaccacgct gctgaaattg ctgctgggcg aactcgttcc gcgttatggc tcgatccgcg
    12241 tgttgggcgg cgcacccgct gaaacgcgca cgcgcgtcgg ctacgtgccg cagtctttag
    12301 cctttgatcc attggtgccg gtcagcgcgc gcgaagtcgt gcttatgggc cgcgtgggac
    12361 gcgcccggtt cggcttgtac ggcgggtcgg accgcaggcg ggcggacgat gcgttggcgc
    12421 gcgtggggtt ggaggacttc ggccgccggg cctttgccga actctcggga ggcgaacgcc
    12481 agcgggtgtt gatcgcgcag gccctggcgg gcggctcgga gttgctgctg cttgatgagc
    12541 cggtcgcgaa cgtcgatccc gaacagaccg aacgcctcta tgcgctcttt caggagctga
    12601 ccgccacggt gacggtgctg atggtctcgc acaatctgag tgtggtcacg gatcgcgcga
    12661 cgcatgtgct ttgcgtcaac ggcaccgccg aactgcatgc catcaacgag gtcgcctcct
    12721 ccacctttgc cgctgcctac ggcggacgcc tgacgacgat ccgccacgac gcctcctgtc
    12781 atgtgcttga cgcttccgga gtcatgcacc ggccccacca tggggaagaa ggatgaaggg
    12841 ggagggaatg ggcgaaaaga acaaaacggg ggcatgatgc tttttttcga ggatttcctg
    12901 cgtttcccct ttctccggca cgccgtgctg gccgtggttc tggcgtcggt cgcggccggg
    12961 ctggtcggca gcctggtggt ggtgcgccgc gccacgtatc tggccggggc cgtttcgcac
    13021 agtctgctgg cggggctcgg tttggcgcgc ctgctgcagc gccgtttcgg gatcgggttc
    13081 ctgaccccca tgggtggcgc ggtgctgaca gcgctggccg tggttctgct ggtgacgcat
    13141 ctgacggcgc gcggccgaca ccgcaccgac accgtcctca gcgcggtgtg gaccggcggc
    13201 atggcgctgg gtgtttcttg cgtggcggcc acacccgggt atcaggatga cctgatgagt
    13261 tatctgttcg gctcgctgct gctggtgacc ggcacggacc tcttctggat ggcggtgctg
    13321 gacgcagtgc tggtggcggt gatcctcctc tgccacaatc gttttctggc gatcgcgttc
    13381 aacgaggagc tggcgcgtct gcgcggggtt aacaccgcgc tctacgaact gctttttcac
    13441 atcatgaccg cgctgaccgt ggtgttgctg gtgcgggtgg tcggcatcgt tctggcgatc
    13501 gccctgctta cgctccctgc cgcggccgca gggcgtctga cgcggagtct ggaccgcatg
    13561 atgctgctcg ccgcgggact ctgcctgttg gttagcctga tcgggcttgt ggtgagctac
    13621 gcgcccggct ggccgccggg cgcaacgatt gtggaactcg cggtccttgt ctatctcggc
    13681 acttcgctga cgcgtgcgcg gcggtgaacg ttctgcggag gaccgacggg ttttgacaac
    13741 cgaaagggga tgccgtacac ttcggattta cgaatgcgag ccttctcacc cctatatcgt
    13801 aaaggaacgt catggctact gatttgaaag ccgcttacaa gaccatcatg gatgaccact
    13861 tcactccgca gatggaaatc actttcctcg acggcgggaa acgacagacc ctgtgttacg
    13921 aaaaggtgtc ctgggttatt gacggagagg agaaagggct gcggtacggc gagaatccgg
    13981 gacaggaggc cgcgctgtac aagcttgtga acggcaacct cacgctcggc gaggtcacgg
    14041 ccctgacgcc cgggaacgtt ctcgcctcgg agcccgagct gctccagtcc gggaaacatc
    14101 cgggcaaaac caaccttacg gacgccgaca acgcgctcaa catcctgcgc tatctggatg
    14161 ccacgccctg cgccgtgatc gtcaagcaca acaacccctg cggggtggcc aaaggcccga
    14221 ccctcgccga ggcgtacttc cgcgccaaca aggctgaccg gctcgccgcg ttcggcggct
    14281 gtatcgcact caaccgcgag gtggacattg agaccgcccg gctcgtggcg gaaaactatg
    14341 ccgaggtggt cgtggccccc gagttcgccc ccggtgtgat ggatctgttt gccaccaaga
    14401 aaaacctgcg tgtgatgcgc atccgcaaca tggcgcgcct cacctcgttc gttgcgcggc
    14461 gtgtggtgga tttcaaatcg ctgatcgacg gcggcctggt ggcgcagtgg tcgtttgtac
    14521 cggaagcgcg cacgccggag caactcctgc ccgccgaatg cacgaccaag gacggcacag
    14581 tccaccgcat ccggcgtctg cccactccgc aggagtacga ggatctgatc ttcggctggt
    14641 tggtcgaggc aggggtcacc agcaattccg tgctctatgt caaggacggc gcgaccgtgg
    14701 ggatcggcac tggcgagcaa gaccgtgtgg gcgtggccga gatcgcgcgc gacaaggctt
    14761 accgcaaact ggccgactgg atcgcttggg agaagtatgc caccccgtac aacgaactcg
    14821 tcctgaagtt cggcgatctg ggcaaaagcc ggatggggga aatcgacgaa gaggtcaagg
    14881 cccgcaaagg cggtctgccc ggatgcagca tggtctcgga cgccttcttc cccttccgtg
    14941 acggtgcgga ggtcggcatc cgggagggga tcacttcgat tgtccagccg ggcggcgctc
    15001 tgcgcgactg ggatgtgatc gactgctgta acgaggctgg cgtcgcgatg gtcttcaccg
    15061 gtcagcgcag cttcaagcat tgaggcgaaa gcggcgttca gccgcaatcc ggctggcgtg
    15121 tcgttttcgg tgtgtgtgcc gcgatctgaa ggcacatcag cgcccagaaa ccgccgatca
    15181 gtccggcgac atgcggctgg agcgcggtcg gcaggctgta gatcacgcac aggctgggga
    15241 tccagaccgt ccagttcatc accagatttg gcaggaccag tctggagtac catccccggc
    15301 ccatgtgcgg tgcgaccgct tcccaacggt acccgcagtc tttccagaaa tgcgccagcg
    15361 aaacgagcgg caccgtgaga aacgcggaaa agacgaactg atccgtgaga atcttgcaga
    15421 ccagggtctg cacgtcggtg ccgttcccgt acaacgcgct ctgcagacgg tagaattgcg
    15481 ggatgaggag ccccatcagg ccccagaacc ccagcgcaaa agcgagcgcg cggccacgtt
    15541 gggccggacg cagcgcggga caggccagac agaacaggta aggcaccacg ccgcagaaga
    15601 agagatagct ggccagtgaa aaaggtaccc cgaaccgggc ctgccagcgg ccgacctccg
    15661 tcagcgcgtg gcggaccggc ggcagaaacg tatatgaggc cagcaaggcg aacgccgccg
    15721 cctgcaaaat cagtcccggc accagattgg cgcgcgcggc tttcaagccg atgatccacg
    15781 gatttgtttg catccgtata agtataaacg atctgttccc gtgtggtcaa agcccgtgcg
    15841 gacgccattt gcaatcggtc agagacaggg ggtgtgcgaa gacaatacct aaaaagagca
    15901 gttgaacctt caacgctctt tggtttcaag gtttcagggt ttctggtttc agggtttcag
    15961 ggtttcatcc aaatcgggat cgggatcgct atcggtatcg ggatcgggat cgggaacgtc
    16021 gacccgcgac attcgacccg cgacgatcga cagggacgcc cgcgtttccg acaccgcagg
    16081 tcgctcgtcg cgggtcgcgg gtcgacataa ggtctgacgg gagaagtgaa aaactccttg
    16141 cctataaatt gccaggatta cttgacagga ttacaggatt ttcaggattg aacaacatca
    16201 tgttaatcat gtcctaaact tacttccaag gttttttcgc ggcagagccg cgcggcggg
//
LOCUS       DAOSRB010000046        16100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3730_length_16100_cov_7.504581, whole genome shotgun sequence.
ACCESSION   DAOSRB010000046 DAOSRB010000000
VERSION     DAOSRB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16100
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3730_length_16100_cov_7.504581"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..553
                     /locus_tag="PLZ74_04090"
     CDS             59..553
                     /locus_tag="PLZ74_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOR97534.1"
                     /translation="MLKLIRTSLSREAHPLVQFIKYGVVGGMATGVQILTFFLCGWFL
                     FPCLTQDDIVVRLLGLTAPAMSASVRAWNAGFCNAIGFLFSNTFCYVLNRLFVFKPGR
                     HHWVMEFLLFFAVSGISMVLGTAIQSFLITYQGIQTTFAFGANILCALLINYAMRKFV
                     IFKG"
     gene            complement(587..2617)
                     /gene="metG"
                     /locus_tag="PLZ74_04095"
     CDS             complement(587..2617)
                     /gene="metG"
                     /locus_tag="PLZ74_04095"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019570578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOR97535.1"
                     /translation="MGNTKRKMVVTSALPYANGDIHIGHLVEYLQTDFWVRFQKMRGH
                     ACVYVCADDTHGTPIMVRARAEGISPEELIARSYERHTRDFADFEIQFDQYHSTHSEE
                     NRALCNTIFESMRQSGAVTVRKLPQLYCEQDAMFLPDRFVQGVCPKCGAAGQYGDACD
                     VCSQTYPAEELKEAHCTLCGNVPVTRDSEHLFFELEPFRDFLKAWIPDHTAPDVANKL
                     LEWFNEPLRGWCISRDAPYFGFEIPGYPGKFFYVWVDAPIGYMASLQKWCALHGESFD
                     AWWQQPGGELYHFIGKDIVRFHCLFWPAMLHTADYKTPDQVFVHGFLTVNGEKMSKSK
                     GTFIKARTWLEHLKPQDLRFYYACKLNGTTDDMDLNLGDFISRVNSDLVGKITNLASR
                     GAQMLHKSLGGRMGSMDVEGRALYEKAVGKAERIAAHYENRDFSKVMVEVRDLADQAN
                     QYFDGQAPWALVKTDPEAARAVMTSILNLFRVLTIYLQPVLPGYAARVAALFNEQPYV
                     WPDIARPVENLPIGPYVYLATRVEQASVERLIEASREPERSAVPAQSAKPAAQPAEPV
                     RETIDYDTFSQMDLRVAEVLSAEPVEGADKLLKLQLDIGEERPRQVFAGIRRHCDAAS
                     LVGCRVVMVANLAPRKMRFGVSEGMILAAGNPDGTLFVIQADPGALPGATVK"
     gene            complement(2827..4122)
                     /locus_tag="PLZ74_04100"
     CDS             complement(2827..4122)
                     /locus_tag="PLZ74_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOR97536.1"
                     /translation="MTITEKILARESGASVVSPGDNIWVNVGILLTHDVCGPGTIGIF
                     HREFGKDAKVFDREKIVILPDHYIFTADEKAHRNVDTLRVFVKEQGLPYFYDPGTENY
                     CGVCHVAMPELGHVRPGETIFGTDSHTCTHGALGAFSTGIGNTDAGFIMGTGKLLVKV
                     PPTIRFVLDGELPPYLSAKDLILHIIGDISVDGATYHAMEFTGSAIEALSIDERCTIC
                     NMAVEAGAKNGIIAPDRKTIDYVKARTKKPFEPLFSDPDAKVADERRYDVSRLEPVVA
                     KPHLPDNKALARACSDVKIDQVYIGSCTGGKLEDFIMAAKVMQGKRVAVRTLLVPATR
                     QVEKGLDETKIGGQSLRAIFEEAGVEPIAPPSCAACLGGPVDTFGRTHGHEVVVSTTN
                     RNFIGRMGSKDARIYLASPYTAAASSLTGHITDPRTVRL"
     gene            complement(4119..4826)
                     /locus_tag="PLZ74_04105"
     CDS             complement(4119..4826)
                     /locus_tag="PLZ74_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97537.1"
                     /translation="MSKKPTQFDFWFAVNNTRIVVPPKRHLETFGNTIIRYHLVSELM
                     DTVGKVRVREGRMQALRPQIITPSSYSTMILEGFGEQAQRYVEWLKEHEDTVRILRYG
                     YTLKQEAFSEQVVSDATEAVLERVKAEAEAHNDPFAAVIHGVDDPWDVCLVRLFWTVI
                     HNSAQENIRELHERRLFEMQDGIPAGLREEIERGFQAAAQDAKLIKPLGRLLQEHGLF
                     ERYQDRFFSLVQGKGGT"
     gene            complement(5119..8889)
                     /locus_tag="PLZ74_04110"
     CDS             complement(5119..8889)
                     /locus_tag="PLZ74_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97538.1"
                     /translation="MKNRKFHMFSAVGLTGCLLFGAAGAALADTIYKADTATLNTSAD
                     WVGGTNASIEHIGRFDSLLSAANAANLSLGADLALGGLYFANNLKGPVSIAAGNTLTL
                     GASGISASSANQTVTFNHDIQLAADQTWRAANKALTLNGDLAGDAGITLIKTGKNDYN
                     NLWSLTASNTFAGTVDIREGHLRLYNTTGSLIWADVIVSTGAEFRVNGTAGIAPAEGL
                     RGKRVTLNGGLLRLTGPGTMPIHETFSGPLRIGPGFSEVLAIPDTNPLKPVRLTFQRL
                     ERESGGVVFLHGSNLGDQPFGVSDSRYANIEFIEPPTLVGGGGAYNTPNASIIPYAVC
                     NPYSSDPPYYFATYESGYGVRQLKSYASGILDGSVTTTNARLTTTATLNQSTTINALI
                     LEGEGSIEGPGTLTLTSGALMSAGNRSVNSIGINTPGTLAFGTAEAILNFRYRTNTLV
                     IGSAVTGSAGLTKGSAGNLSLLGPVDMGGVIDFANGRLTVGDTVNGHIRLSRDGTLEM
                     LSGSSISGAVSVSGGNVYLTNTCAISGALDIGESRTVTMSNGGSVTGRITVFSGGTLT
                     LNSGSVISGNLTNFGTFNYNSTDTEPITISLDGLGTVYQRNVNMPLTLFQSPGEHWVG
                     NIVPTEGATVILDGEPDATTVVNNMLPEPARTKWTIENGVWIFNPLHPSKNLSADVTL
                     NGGVLTVTPETRRFTFYTAGGTNTLTITGGVLMDQPETDWSLRFGNHHGSWEQTGCNF
                     IGVQTGGIVLVQSKEYLHLGSLADHTTATYDLSGGTVYAFGQSDNAVSIGSGTNNNSL
                     AAFTLRGSGKLVSRKRIYGNRAEEGANQVFAFQGGTLATASYEAVNLRSEPGGARGTL
                     FNEGGTLAPGDFGVPGRTLVNGSYTNAPNAVIALDIGGASQANSFTNGAGYHDHLMIT
                     GNAVLDGALRISLINGFLPDPASLFYAISCDNPMTFAAGGFSNAEDNQVWAEDGFARF
                     DVTLSNDSKHLLLGNAALNQWAGASGGDWDASGAWSLTNPATAEFGAYFGPNLTAAGT
                     VTAEAGRTLRGLAFNNAVSYTVAGPGALTLASDGAYGMPRILSLLGSHTLTAALVLQD
                     ALTVDVANAAGTLTLAGGVTGGKALTKLGAGTLALAGRNVLGAVTVAAGAAAQTGGVS
                     AFDALEVSPGGVFRLVSGTLTLAQGLTVAPGGAFDFGSYGGGTLRLAVGAPGGAVDTA
                     TEFWEAVADGRVTVKGQAAADPSLFILKEEQSGGVTYVTVTLKSFGTLLQFR"
     gene            9527..10900
                     /gene="dnaA"
                     /locus_tag="PLZ74_04115"
     CDS             9527..10900
                     /gene="dnaA"
                     /locus_tag="PLZ74_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOR97539.1"
                     /translation="MNAAAELWANACNHLKSVLNADVFSRWIEIIRPATLEENCLSLE
                     VENDFSQAWLEDNYKGFILDALHLAGAPSELSVTFVVNAPEAQEGLPDDPPQETKPAP
                     RRRPKTPNRTSSNQLNPIFTFENFVTGPSNSFAHAAALGVSQSVGRAYNPLFIYGQTG
                     IGKTHLMQAVGHRVLSSPGMSVFYVTTETLLNEYVEAIQNRTTMEFRKKYRKTDVLLV
                     DDIQFLAGKDSLQAEFFHTFNVLYDAHKQIIMTSDLPPRELSGLEPRLVSRFEWGLVT
                     EIESPDFETRLAILRYKHSMSKVQLPDEMLVFIADNIRSNVRSLEGALTRAITFASLN
                     GTITLDSLRNLLKDQLDDERQKDLTFDDIQRMVADYYDLRMTDMSSKRRPRSVAAPRQ
                     IAMFLCRKLTRSSLPEIAKSFDKTHATVVHACKTIQDRIQTEEDLRDSVREITRKLGR
                     DPVSFQI"
     gene            11211..12305
                     /gene="dnaN"
                     /locus_tag="PLZ74_04120"
     CDS             11211..12305
                     /gene="dnaN"
                     /locus_tag="PLZ74_04120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOR97540.1"
                     /translation="MKLTIMRSAFLDGLQTVQNVVSSKGVLQILSNALLQAKDGKLSI
                     TTTDLDIAERCEVECEVEREGSTTLPVRRLVGMIRELPEGKVLVEVNDQDEATVQSGS
                     SFYKIIGLPTRDYPPIPATEGKFCYRIDQGAFREMLRKTSYAVSQDETRRVLNGVLMA
                     FKENKLTLVATDGRRLALVEHEIEFPPEAETEMILPIKAVSELMRILSNDGDLKIYAQ
                     KNQAVFELGTTMLSSKLIDGVYPNYRQVIPAACDERVVIERDALLAALRRVSVVTTDK
                     CNATRLTFSANQLIVSINTPEVGEGRDVVPVKYGGKEISIIFNPEFVMDPLKNMDDDE
                     VFIEMNDGHSPALIKCGIPFLYVLMPLRIS"
     gene            complement(12273..12902)
                     /locus_tag="PLZ74_04125"
     CDS             complement(12273..12902)
                     /locus_tag="PLZ74_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97541.1"
                     /translation="MTYRYRRDAVRLPDAQLHHITLRLAFYESHVDGAALLFLTAREP
                     LTALRLDVRDLEIFEVAVSLAETLSPDDRERFAEPFPPTDGFSACAHTLDTVARKLVI
                     PLPRPLAPGASLRVYVRCRCVPSDALLEGLYRDVTPPGAPQQYISQCQHARAYRPAPP
                     PFRGRASGGLSGRGSVTWAEPSMKHRQDVRATAETKQKLLTDPQGHQHV"
     gene            complement(12942..13259)
                     /locus_tag="PLZ74_04130"
     CDS             complement(12942..13259)
                     /locus_tag="PLZ74_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97542.1"
                     /translation="MRRNTRLRIFGWLACAAVAHAQAPAKRPPPEQRGERSPPAVVTN
                     AAPHAAQTNAAAKARTPAKPTFTRKQVERRLRMLQELRDRGLILQPFYERKVRECEEG
                     LVE"
     gene            complement(13282..15609)
                     /locus_tag="PLZ74_04135"
     CDS             complement(13282..15609)
                     /locus_tag="PLZ74_04135"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOR97543.1"
                     /translation="MMRRGILWIVGLTGCAAASVLGAPGEVTLAERGKADGMAIVRSE
                     EASASQRHAAEELQRFTEQMTGVRLPIVTDREPLPARAVLLGVTRHTAAILGTAADAA
                     AFGDDGFRIVTRPPHLLILGGPVRGTLYGVYELLERVGGCRWYASWHSVIPRLERFTV
                     PNTDETQKPAFALREPFWFDMFDGDLAARNKANGNAMRLTERHGGKIRFGGGLFCHTF
                     NRLCPPEEFFETHPEYFSEIDGRRIKEPSQLCLTNPDVLKIVTTRVLERIRKDPTAKL
                     FSVSQNDWRYPCTCPACKAIDDREESHAGTLITFVNQVAEAVEREFPEVWIETLAYQY
                     TRKPPKTVRPRRNVVPRLCTIECDFSYPLDQSAYEQNRKFVEDIRGWSAMTDKLYIWD
                     YTTNFRHYTAPFPNVLALRDNVRFFRDHRVVGLFEQGAYHGRHGDFAELKAWLLAKWL
                     WNPDLPTEPLLADFFAGYYGAAAPLVRQYFDELHTFYNDPAKKPLRIFDDVRSPVLPD
                     AFLDRAADLWSRAEAAVRDSPAHAYNVRMGAFSVLYARLSRLPASEQKTVWVTSNPQQ
                     YAVPAEYRALATELLKRFDEARNIRLAESLERHTKTIADWRALISPPPLPPPGDRATI
                     EDEQLSLGRPGVWGEKKVADPQAGDGFALKLFNTHYEWCTSLPFGKVAFDPGARYRIR
                     IRLRVERVPGQEGTAFWAGVYDPVNKKGHGKTVEPKTSAIGDGYAWYDVAEWVPESAH
                     YVWIGPGRFDKKTQNANPAIRAVYIDCLELQRLEK"
     gene            complement(15921..>16100)
                     /locus_tag="PLZ74_04140"
     CDS             complement(15921..>16100)
                     /locus_tag="PLZ74_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97544.1"
                     /translation="SCQFIGKELGVFSLRRSEFGVGRWAFKGGGVKRRPDGQTLVPPS
                     YGQKHKNKQSVQICG"
BASE COUNT         3045 a         5003 c         4806 g         3246 t
ORIGIN      
        1 gaaaccccga aaccaaagag agtggctccg ttgcgacggt tatggtatga tcccgcccat
       61 gttgaaactg attcgaacga gtctgagccg cgaagcccat ccgctggtgc agttcatcaa
      121 gtacggcgtg gtgggcggta tggccacggg ggtgcagatc ctcaccttct ttctgtgcgg
      181 atggtttctc ttcccctgtc tgacccaaga cgacatcgtg gtgcggctgc tcggcctcac
      241 ggcgccggcg atgtccgcgt ccgtccgcgc ctggaacgcg gggttctgca acgccatcgg
      301 ctttctcttc tcgaacacct tctgctatgt gctcaaccgg ttgttcgtct tcaagccggg
      361 ccgacaccat tgggtgatgg agttcctgct tttcttcgcg gtctccggca tcagcatggt
      421 gcttggtacg gcgatccagt cctttttgat cacgtatcag gggatccaaa ccaccttcgc
      481 gttcggcgcg aacattctct gtgccctgct gatcaactac gcgatgcgca aattcgtgat
      541 tttcaaaggg tgattgcgca aaggggcgtg cgttgtgaag ttctccttac ttcactgtcg
      601 cgcccggcaa cgcgcccgga tcggcctgga tcacaaacag cgtgccatcg ggattgcccg
      661 ccgcgagaat catgccctcc gacacaccga agcgcatctt gcgcggcgcg agattcgcca
      721 ccatcaccac acggcagccc accagcgagg cggcgtcgca gtgccgacgg atgcccgcga
      781 aaacctgacg cggacgctcc tccccgatgt cgagctgcag cttgagcagc ttgtccgcgc
      841 cttccaccgg ttccgcgctg agaacctcgg ccacacgcag atccatctgg gaaaaggtgt
      901 cgtagtcgat cgtctcccgc accggttcgg cgggttgcgc ggccggtttt gcggactgtg
      961 ccggaacagc ggaacgctcc ggctcgcggc tggcttcgat cagccgctcc acagaggcct
     1021 gctccacccg cgtggccaga tacacgtaag ggccgatcgg cagattctca accgggcgcg
     1081 cgatgtccgg ccagacgtac ggctgctcat tgaaaagtgc cgccacccgg gccgcatacc
     1141 ccggcagcac cggctggaga tagatggtca gcacgcgaaa caggttcagg atcgatgtca
     1201 tcaccgcgcg cgcggcctcg ggatcggttt tgaccagagc ccagggtgcc tgaccgtcga
     1261 aatactggtt ggcctgatcg gcaaggtcgc gcacctcgac catcaccttg ctgaagtcgc
     1321 ggttctcata gtgcgcggcg atacgctcgg ccttgcccac ggctttctcg tacagcgcgc
     1381 ggccctccac atccatgctt cccatacggc cgcccagact cttgtgcagc atctgcgcac
     1441 cgcgcgaggc gaggttggtg atcttgccca ccagatcgga gttcacgcgg ctgatgaaat
     1501 caccgaggtt cagatccatg tcatccgtgg tgccgttcag tttgcaggcg tagtaaaaac
     1561 gcagatcctg cggcttgagg tgctcgagcc aagtgcgcgc cttgatgaag gtgcccttgg
     1621 atttgctcat cttctcgccg ttgaccgtga ggaaaccgtg cacaaaaacc tggtccggcg
     1681 tcttgtagtc ggccgtgtgc aacatcgcgg gccagaagag acagtggaag cgcacgatgt
     1741 ccttcccgat gaaatggtac agctcgcccc ccggttgctg ccaccaggcg tcgaacgact
     1801 cgccatgcag ggcgcaccat ttctggaggg aggccatgta cccgatgggc gcgtccaccc
     1861 acacgtagaa gaacttgccc ggatacccgg gaatctcgaa gccgaagtag ggggcatcgc
     1921 gcgagatgca ccaaccgcgc agcggctcgt tgaaccactc cagcagcttg ttggccacat
     1981 ccggcgcggt gtggtcaggg atccacgctt tcagaaaatc gcggaacggc tcgagttcga
     2041 agaaaaggtg ctcgctgtcg cgcgtcaccg gcacgttgcc gcacaaggtg caatgagcct
     2101 ccttcagctc ttcggcggga taggtctgac tacagacatc gcaggcgtcg ccgtactgtc
     2161 cggccgcgcc gcacttcgga cagacgccct gcacaaaacg gtccggcaga aacatcgcgt
     2221 cctgttcgca gtagagctgc ggcagtttgc gtaccgtcac ggcaccgctc tggcgcatcg
     2281 actcgaaaat cgtattgcag agagcgcggt tttcctcgct gtgggtggag tggtactggt
     2341 cgaactggat ctcgaaatcc gcgaaatcgc gggtgtgccg ctcgtagctg cgtgcgatca
     2401 gctcctccgg ggagatcccc tccgcacgcg cgcgcaccat gatcggtgtg ccatgcgtat
     2461 cgtccgcgca gacataaacg caagcgtggc cgcgcatctt ttgaaaccgg acccagaaat
     2521 ccgtctgcag atactcgacc aaatgcccaa tgtgaatgtc cccgttggcg tacggaagag
     2581 ccgacgtcac caccatcttg cgttttgtgt tgcccataaa gtgctcccac ggtaaaaaaa
     2641 cgaagtgtta tcatagcgtg tccgccccat tcccgccact tcttagttca tcagtgcctt
     2701 acgaaccaac gagcacaata aaaacaagtt gtttcaccct cgttctttga gattgcgggc
     2761 tccgcccgcg ttgggggttg aggctgcaag atttgtacag cctcacagtc tcacagtctc
     2821 acaacctcac agcctcacag ttctcgggtc agtgatgtgg cctgtgaggg atgaggccgc
     2881 cgcggtgtag ggcgaggcga gataaatgcg cgcgtccttt gagcccatgc gtccgatgaa
     2941 gttgcggttg gtcgtgctga ccaccacctc gtgtccatgg gtacgcccaa aggtgtcgac
     3001 cggcccgccc aggcaggccg cgcaggaggg cggcgcgatc ggctcgacgc ccgcctcttc
     3061 aaagatcgcc cgcagactct gtccgccgat cttggtttca tcaagcccct tctccacctg
     3121 ccgtgtggca ggcacaagca gcgtgcggac ggcgacccgc ttgccctgca tgactttggc
     3181 cgccatgatg aaatcctcca gcttgccgcc ggtgcaggaa ccgatgtaga cctgatcgat
     3241 tttcacgtcg ctacaggcgc gagccagcgc tttgttgtcg ggaagatgcg gcttggccac
     3301 aaccggctca agccgggaga cgtcgtagcg gcgctcgtcc gcaactttgg cgtccggatc
     3361 gctgaacaaa ggctcgaacg gcttcttcgt gcgggcctta acgtaatcga tggtcttccg
     3421 gtcgggcgcg atgatgccgt tcttcgcgcc ggcctcgacc gccatgttgc aaatcgtgca
     3481 gcgctcatcg atgctgaggg cttcaatcgc ggaacctgtg aattccatcg cgtggtaggt
     3541 cgcgccgtcc acactgatgt caccgatgat gtggaggatc agatccttgg ctgagaggta
     3601 cgggggcaat tcgccgtcca acacgaaacg gatggtcggc ggcaccttga ccaacagctt
     3661 acccgtgccc atgataaacc ccgcatcggt gttgccgatg cccgtcgaaa aggctcccag
     3721 cgccccgtgc gtgcaggtat gggaatccgt gccgaaaatg gtctcgcccg gacgcacatg
     3781 gcccagttcc ggcatcgcca catgacaaac tccgcagtaa ttttcggtgc cggggtcata
     3841 gaaataaggc agcccttgct ctttgacgaa cacgcgcagc gtgtccacgt tgcggtgggc
     3901 tttttcgtcc gccgtgaaga tgtagtgatc cggcagaatc acgatcttct cgcgatcaaa
     3961 aaccttggcg tctttaccga attcgcggtg gaaaatgccg atcgtgccgg gtccgcacac
     4021 gtcatgcgtc aggaggatcc cgacgttcac ccaaatgttg tcgccgggcg acaccacgct
     4081 cgcgcccgac tcgcgggcca gaattttttc cgtgatggtc atgttccgcc tttcccctga
     4141 accagactga agaagcggtc ctgataccgc tcaaagagcc cgtgctcttg cagcagtcgg
     4201 cccagcggtt tgatcagctt cgcgtcctgc gccgcggctt ggaacccgcg ttcgatctct
     4261 tcgcgcagac cggccgggat gccatcctgc atttcgaaca gacgccgctc gtgcaattcc
     4321 cgaatgttct cctgagccga attgtggatc acggtccaga aaagccgcac cagacagaca
     4381 tcccacggat cgtccacccc gtggatcacc gccgcaaacg ggtcgttgtg cgcttcggcc
     4441 tcggccttca cacgctccaa caccgcctcc gtcgcatccg aaaccacctg ttcgctgaag
     4501 gcctcctgct tgagcgtgta accgtaccgc agaatgcgca ccgtgtcctc atgctccttc
     4561 agccactcga cataacgctg cgcctgttcc ccgaacccct ccagaatcat ggtggagtaa
     4621 gaggaaggcg tgatgatctg cggccgcaac gcctgcatgc ggccctcccg gacccgcacc
     4681 ttgccgaccg tgtccatcag ctcgctcacc aaatgataac ggatgatcgt gttgccgaac
     4741 gtttccaaat gacgcttggg cggcaccacg atccgcgtgt tgttcaccgc gaaccagaaa
     4801 tcgaattgtg tgggtttctt actcataaca tgagaaggga gtataacagg aataccggat
     4861 tctgaaaagc gcgagaaaag acccggttcc cgcgcgttga gtgaccggcc ccgcccgtaa
     4921 cggcggatca aacggaccgg tcggacacac gcgttcttgc cgatcgtcgc gggtcaacgt
     4981 tcagcattgc cgtcacttcg ggcgtccgag acgtccgccc gcactttacc gccggagcgg
     5041 acggttcgcg aaaccatcct cacgaggacg agatggcgat cccgatcccg acagcgattt
     5101 cgataaaccc ggaacccatt acctgaactg gagcagcgtg ccgaagctct tgagggtgac
     5161 cgtgacatag gtcaccccgc cggactgctc ctccttgagg atgaagagcg aggggtcggc
     5221 cgcggcctgg cccttgaccg tgacccggcc gtccgccacc gcctcccaga actccgttgc
     5281 cgtgtcgacc gcgccgcccg gagcgccgac agcgaggcgc agcgtgccgc cgccgtagct
     5341 gccgaagtcg aacgcgccgc ccggagcgac cgtcaggccc tgcgcgaggg tcagggtccc
     5401 ggagacgagg cggaagaccc cgcccggcga gacctccagc gcgtcgaagg ccgagacgcc
     5461 gccggtctgc gccgccgcgc ccgccgcgac cgtcaccgcg cccaagacgt tccgccccgc
     5521 gagcgccagc gtgcccgcgc cgagcttcgt cagagccttg ccgcccgtga cgccgcccgc
     5581 gagggtcagg gtcccggccg cgttcgccac gtccacggtc agcgcgtcct gcaggaccag
     5641 cgccgcggtc agggtatggc tgcccagcag cgagaggatg cgcggcatgc cgtacgcgcc
     5701 gtccgaggcc agcgtcagcg cgcccggccc cgccaccgtg taggagaccg cgttgttgaa
     5761 ggccagcccg cgcagcgtgc ggccggcctc ggccgtcacg gtcccggcgg cggtcaggtt
     5821 cgggccgaag tacgcgccga actccgccgt ggcggggttg gtcaggctcc aggccccgga
     5881 cgcgtcccag tcgccgcccg aagccccggc ccactggttc agcgcggcat tgcccagcag
     5941 gaggtgcttg ctgtcgttgg agagggtcac gtcaaagcgc gcgaaaccgt cctcagccca
     6001 gacctgattg tcctccgcgt tggagaagcc tcccgccgcg aatgtcatcg gattatcgca
     6061 actgatcgcg tagaagagcg acgccggatc gggaaggaag ccgttgatca gcgaaatccg
     6121 cagcgcgccg tccaatacgg cgttcccggt gatcatcagg tgatcgtggt accccgcacc
     6181 attcgtgaag gaattggcct gggacgcgcc gccgatatcg agggcgatca ccgcgttcgg
     6241 cgcgttggtg tagctgccgt tcacgagggt ccggcccgga acgccgaagt cgcccggcgc
     6301 gagcgtgccg ccctcgttga acagcgtccc gcgcgcgccg cccggctccg agcgcaggtt
     6361 gaccgcctca tacgaggccg tcgccagcgt gccgccctgg aaggcgaaga cctgattggc
     6421 cccctcttca gcacggttcc cataaatgcg cttacggctc acgagtttgc cagaacccct
     6481 cagcgtgaag gcggcgaggc tgttgttgtt cgttcccgag ccgatcgaga ccgcgttatc
     6541 gctctgcccg aacgcataca ccgtgccgcc agataggtcg taggtggccg tcgtgtgatc
     6601 cgccagactt ccgaggtgca gatactcttt gctctgcaca agcacgatgc cgcccgtctg
     6661 cacgccgatg aaattgcacc ccgtctgttc ccaggaacca tggtgattgc cgaagcgcaa
     6721 gctccaatcc gtctccggct gatccatcag cacccccccc gtaatggtca gcgtgttggt
     6781 gccgcccgca gtgtaaaagg tgaacctgcg cgtctccggc gtgacggtca ggacgccacc
     6841 gttgagcgta acgtcggcgc tcaggttctt agacggatgc aacgggttga agatccagac
     6901 tccgttctcg atcgtccact ttgtccgcgc cggttcggga agcatgttgt tcaccacggt
     6961 cgtcgcgtca ggctcgccgt ccaaaatgac ggtcgcgccc tctgtcggga cgatgttgcc
     7021 gacccagtgc tcaccggggc tttggaaaag cgtgagcggc atattgacgt tacgctggta
     7081 gacggtgccg agcccgtcga gcgagatcgt gatgggctcg gtatccgtgc tgttgtagtt
     7141 gaacgtgccg aaattggtca gattccccga gatgaccgag ccgctgttga gcgtcagggt
     7201 gccgccggaa aaaacggtaa tcctgcccgt cacgctgccg ccattcgaca tcgtcaccgt
     7261 cctgctctcg ccgatgtcca atgcgccgga gatggcgcag gtgttggtca gatagacgtt
     7321 accgccggac acggagaccg ccccggatat cgagctgccg gagagcatct cgagagtccc
     7381 gtcgcgggaa aggcggatgt ggccgttcac ggtgtcaccg accgtcagcc gaccgttggc
     7441 gaaatcaatg actcctccca tatccacggg gccgaggagc gaaaggtttc ctgcagaacc
     7501 cttggtcagc cccgccgaac cggtcacggc actgccgatg accagcgtgt tggtacggta
     7561 gcggaaattg agaatcgcct cggcagtgcc gaacgcgagg gttcccggcg tgttgatgcc
     7621 gatgctgttt acgcttcggt tcccggctga catcagggca ccgctggtca gggtcagggt
     7681 accggggcct tcgatgctgc cctccccttc taagatcagg gcgttgatcg tggtagactg
     7741 gttgagcgtc gccgtggtgg tgagacgggc gttcgtggtg gtgacgctgc cgtccagaat
     7801 gccggaggcg taagatttga gctgacgaac gccgtagccc gactcgtacg tcgcgaagta
     7861 atacggcgga tcggagctgt aggggttgca caccgcatac gggatgatgc tcgcgttggg
     7921 agtgttgtag gcgccgccgc cgccgaccag cgtgggcggt tcgatgaatt cgatgttggc
     7981 gtaacggctg tcagacacgc cgaacggctg atcgccgaga ttactgccgt gcaggaacac
     8041 gacacccccg gattcacgct ccagccgctg aaacgtcaac ctgaccggct tgagcggatt
     8101 cgtatccgga atcgcgagca cttccgaaaa gccggggccg atgcgcagcg gtccgctgaa
     8161 ggtctcgtgg atgggcatcg tgccgggtcc ggtcaggcgc agcaagcccc cgttgagcgt
     8221 cacacgtttc ccccgcagcc cctcggccgg ggcgatgccg gccgtcccgt tgacgcggaa
     8281 ctccgcgccg gtgctgacga tgacatcggc ccaaatcagc gaccccgtgg tgttgtagag
     8341 ccggagatgc ccttcgcgga tatccactgt tccggcaaac gtgttcgagg cggtcaggct
     8401 ccagaggttg ttataatcgt tcttcccggt cttgatcaat gtgatccccg catcgcccgc
     8461 aaggtcgccg ttgagcgtca acgctttgtt cgctgcacgc caagtctgat cagccgcgag
     8521 ttggatgtcg tggttgaagg tgacggtctg gttggcggag gaggcgctga tacccgaggc
     8581 accgagggtc agggtattcc cggcggcaat cgacaccggc cctttcaggt tgttcgcgaa
     8641 atacagcccc cctaacgcaa ggtccgcgcc caacgaaagg ttcgcggcgt tggccgcgct
     8701 gagcaaacta tcgaaccggc cgatgtgctc gatcgaggcg ttcgtgccgc cgacccagtc
     8761 ggcggaggtg ttcagggtcg cggtgtcggc cttataaatc gtatcggcca gcgcggcacc
     8821 ggcagccccg aagaggagac aacccgtcaa cccgacagcg gaaaacatat ggaatttcct
     8881 gtttttcatc gtctttcacg ctccttcttt tcgccactct ttgtcagcaa gccctgtccc
     8941 caacgatggt aatcgtaacc ccaaccaagg caggaagtca accccgctga cgtttaacct
     9001 aaaaagagca gttgaacctc caactctctt tggtttcaag gtttcgtggt ttcagggttt
     9061 cagggtttca aggtttcagg gtttcatcca aatcggggtc gggatcggta tcgggatcgg
     9121 tatcgtcgac ccgcgacccg cgacattcga cccgcgacga tcgacaggga cgcccgctgg
     9181 tagggacggt tccccgaacc gtccgcggac gcctcagtcc cgcatacgca ttcggttaaa
     9241 aaaatcatat taatcctgtc atcctgatgc cctgaacggg gggcttgttg ataattttaa
     9301 cccggggtct gatcatattg gggatatcgg gggttttttt gcctttaact gttcttttca
     9361 gaagaagtta cattatctac agcaccctgt tgataacctg ttggccgatt gatcatcttt
     9421 tccccttgtc ccctctcttc aattgtggtt caacagtaca cgttgcgggg gactgttcca
     9481 atgcctcctt ttacctcttt ttccgacctg ttttgtgagt gccgttatga atgctgccgc
     9541 agaattgtgg gctaacgcct gcaatcacct gaaatccgta ctcaacgccg atgtcttctc
     9601 ccgctggatc gagatcatcc gtcccgcgac gctggaggaa aactgtctgt ccctggaagt
     9661 tgagaatgat ttttcccaag cttggctgga ggataattac aaagggttca tcttggacgc
     9721 cctccatttg gcgggcgcgc cgtccgaact ttccgtgacc tttgtggtga atgccccgga
     9781 agcgcaggaa gggttgccgg atgatccgcc gcaggaaacc aaacccgcgc cgcgccgacg
     9841 tccaaaaacc ccgaaccgga cctcttccaa ccagctcaac ccgattttca cctttgaaaa
     9901 tttcgtgacc ggcccgtcga acagtttcgc gcacgccgcc gcattgggtg tgtcgcaatc
     9961 ggtcggccgc gcttacaatc ccctcttcat ctacggccaa accggtatcg gcaaaacgca
    10021 tctgatgcag gcggtgggac accgagtcct ctcctcaccc ggtatgagcg ttttttacgt
    10081 cacgaccgaa acgcttctca atgaatatgt ggaggccatc caaaaccgga ccaccatgga
    10141 gttccggaaa aaataccgca aaaccgatgt gctgctggtg gatgatatcc agtttctggc
    10201 tggtaaggac agcctgcagg cggagttttt ccacaccttc aatgtcctgt atgacgccca
    10261 taagcagatc atcatgacca gcgatctgcc tccgcgtgaa ctctcaggcc ttgagccgcg
    10321 gctggtctcg cgctttgaat ggggtttagt cacggaaatc gagtcgcctg atttcgaaac
    10381 ccgtctggcg attctccgct ataagcactc aatgtccaag gttcaactac cggatgagat
    10441 gctggtgttt atcgccgata acatccgttc aaatgtccgc agccttgagg gtgcgctcac
    10501 ccgagcgatc actttcgcct ccctaaacgg caccataacg cttgattcgc tccgcaacct
    10561 gctgaaagac caactcgatg acgaacgcca gaaggatctc acctttgacg acatccagcg
    10621 catggtagcc gattactatg acctgcgcat gaccgacatg tcgagcaagc ggcgtccgcg
    10681 ttcggtggcc gcgccgcgtc aaatcgccat gttcctgtgc cgcaaactga cccgctcttc
    10741 ccttcccgag atcgccaaat ctttcgacaa aacccacgcc accgtagtcc atgcctgcaa
    10801 gacgattcag gaccgcatcc agactgaaga ggacttacgc gattcggtgc gcgagatcac
    10861 acgcaaactg ggccgcgatc ccgtttcttt ccaaatatga ccgcctcttt ataacctgtt
    10921 gatttcctta tgatttgctt ctcacatctc ttcataaccc ggcagttctt aacagccccc
    10981 tccccctgta caccttttct tctaccttta tcgaagggtt atcaacatga tttcactcct
    11041 gttttgtttt tcatagctta ttcatataaa gcaggttgta gagttatcca caaggttctt
    11101 gcttctctac tattaggact gttctttctt tactttaata cccgtaatag actacaatcg
    11161 caccttttat gggtcattaa cgcaatgacc tggccatggg agagaaaacg atgaaattga
    11221 cgattatgcg gtccgctttt cttgacggtt tgcagacagt gcagaatgtg gtgagttcca
    11281 aaggggtact gcagattttg tcgaatgcgc tcttgcaggc gaaagatgga aaactgagca
    11341 tcaccacaac cgatctggat attgcggagc ggtgcgaggt ggagtgcgag gtggagcgcg
    11401 agggaagcac cacgcttccg gttcggcgtc tggtcggcat gatccgcgaa ttgccggagg
    11461 gcaaggtgct ggtcgaagtg aatgatcagg atgaggccac agtgcagagc ggatcctctt
    11521 tctataagat catcgggctg cccacacggg actatccgcc gattccggcg acggaaggga
    11581 agttctgcta ccgaatcgat cagggcgcgt tccgcgagat gctgcgcaaa accagttacg
    11641 ccgtgtcgca ggatgagacc cgccgtgtgc tcaacggcgt gctgatggcc ttcaaagaga
    11701 ataaattgac gctggtcgcc acggatggac gccggttggc tctggtcgaa catgagattg
    11761 aattcccgcc cgaggccgag acggaaatga tcctgccgat caaggcggtc agcgaattga
    11821 tgcgcattct ctccaacgac ggggatttga aaatctacgc gcagaagaat caagcggttt
    11881 tcgagttggg gacgacgatg ctcagcagca aactcatcga cggcgtgtat cccaactacc
    11941 ggcaggtgat tcctgcggcg tgcgatgagc gcgtcgtgat tgagcgcgat gcgttgctgg
    12001 cagcgctgcg ccgcgtttcg gtcgtgacca cggataaatg taacgccacg cgattgacgt
    12061 tctccgcgaa ccagttgatc gtcagcatca acacgccgga agtcggcgaa gggcgcgatg
    12121 tggttccggt caaatacggc ggcaaggaga tctcgatcat ctttaatccc gaattcgtca
    12181 tggatcccct caaaaatatg gacgatgacg aagtctttat cgagatgaat gacggtcaca
    12241 gccccgcgtt gatcaagtgc ggcatcccgt tcctatacgt gttgatgccc ctgcggatca
    12301 gttaggagtt tttgcttcgt ttcggctgtg gcacggacat cttgcctgtg tttcatggac
    12361 ggttccgccc atgtcacgga accccgtcca gataagccgc cagacgccct gccacggaag
    12421 ggtggcggag cgggtcgata cgctctcgca tgttgacact gcgaaatgta ctgttgcggc
    12481 gcgccgggcg gcgtgacgtc ccggtagagt ccctcgagca gcgcgtcaga gggaacacag
    12541 cggcagcgta cgtaaacccg cagcgacgca ccgggggcca gcggtcgtgg cagcgggatc
    12601 accagcttgc gggccaccgt gtcaagagtg tgggcacacg cggaaaagcc gtcggttggc
    12661 ggaaacggct ccgcaaaccg ttcgcggtca tcgggggaaa gggtctctgc caacgaaacc
    12721 gcaacctcga aaatctccag gtcacgcaca tcgagtcgca acgccgtcaa cggctcgcgc
    12781 gcggtcagaa acaggagggc cgcgccatcc acatggctct cgtaaaacgc gagccggagg
    12841 gtgatatgat ggagttgcgc gtcaggcagg cgcaccgcgt cgcgtcggta tcggtaggtc
    12901 atgttttgtt tcgctcagaa aagccggatg ttacctttgc gctattccac cagcccctct
    12961 tcgcactcgc gcactttgcg ctcatagaag ggttgcagga tcaggccgcg gtcgcgcagc
    13021 tcttgcagca tacggagacg ccgctccacc tgtttgcggg tgaaggtggg tttggccggg
    13081 gtccgcgcct ttgccgcggc gtttgtctgc gcggcgtgcg gcgccgcgtt ggtgaccacc
    13141 gccgggggag accgttcccc gcgctgttcg ggagggggcc tcttggccgg ggcttgggcg
    13201 tgcgccaccg ctgcgcacgc cagccatccg aaaattcgga gccgtgtgtt gcgtctcatt
    13261 gccggatcct cccgcgtccg gtcacttttc caagcgctga agctcaaggc agtcgatata
    13321 gaccgcacgg atcgcggggt tcgcgttttg ggtcttcttg tcgaaccgtc ccggcccgat
    13381 ccaaacgtag tgggcgctct ccggcaccca ctcggccaca tcgtaccagg cgtatccgtc
    13441 gcctatggcg gaggtcttgg gctcgaccgt cttcccgtgg cctttcttgt tcacggggtc
    13501 atacacgccg gcccagaagg ccgtcccctc ctgccccggc acccgctcga cacgcaagcg
    13561 tatccggatg cggtaccgcg cgccggggtc gaaggccacc ttgccgaacg gcagcgaggt
    13621 gcaccattcg tagtgggtgt tgaacaactt gagcgcgaag ccgtccccgg cctgcggatc
    13681 agccactttt ttctcgcccc acacgcccgg ccggccgagc gacagctgtt cgtcttcgat
    13741 ggtcgcccgg tctcccggag gcggcagggg tggcggcgaa atgagcgccc gccagtccgc
    13801 gatggttttc gtatgccgct ccaaactctc cgcgagacgg atgttccggg cctcgtcgaa
    13861 acgtttcagc agttcggtcg ccaaggcgcg gtattccgcc gggaccgcat attgctgcgg
    13921 attcgaggtg acccacacgg ttttctgttc gcttgcggga agtcgggaga gccgtgcgta
    13981 caacaccgaa aaggccccca tgcgcacatt gtaggcgtgg gccggcgaat cccgcaccgc
    14041 tgcctctgcc cgggaccaca agtccgcggc gcggtcgaga aacgcgtccg gcaggacggg
    14101 gctccgcacg tcgtcaaaga tccgcagggg ctttttcgcc ggatcgttgt aaaaggtgtg
    14161 cagttcgtcg aagtactgcc gcacgagcgg cgcggccgcg ccgtaatatc ccgcgaagaa
    14221 atccgcgagc agcggctctg tcggcagatc gggattccac agccatttgg cgaggagcca
    14281 ggccttgagt tccgcgaaat cgccgtgccg tccatgatag gccccctgct cgaacagtcc
    14341 gaccacgcgg tggtcgcgga agaaccgcac gttgtcccgc aacgccagca cattggggaa
    14401 cggggcggtg tagtggcgga aattggtggt gtagtcccag atgtaaagtt tgtcggtcat
    14461 ggcgctccag ccgcggatat cctcgacaaa cttccggttc tgttcgtagg cgctctgatc
    14521 cagcggatac gagaagtcgc actcgatggt gcagaggcga ggcacgacat tgcggcgcgg
    14581 ccgcacggtc ttgggcggtt tgcgcgtgta ctggtacgcg agcgtttcga tccacacctc
    14641 cgggaactcc cgctccaccg cctcggccac ctgattgacg aaggtgatca gcgttccggc
    14701 gtgcgactct tcacggtcgt cgatcgcctt gcacgcggga cacgtgcacg ggtagcgcca
    14761 gtcgttctgg ctcacgctga aaagcttggc cgtggggtct ttgcggatgc gctccagcac
    14821 gcgggtggtg acgatcttca gcacgtcggg gttcgtcagg cagagctggg agggctcttt
    14881 gatgcgccga ccgtcgatct cgctgaaata ttccgggtgg gtttcgaaaa actcctcggg
    14941 cggacagagc cggttgaagg tatggcagaa gagcccgccg ccgaagcgga tcttgccgcc
    15001 gtgccgttcg gtgaggcgca tggcgttgcc gttggccttg ttccgggccg cgaggtcgcc
    15061 gtcaaacatg tcgaaccaga agggctcgcg caacgcgaag gcgggtttct gcgtctcatc
    15121 cgtattcggc acggtgaaac gttcgagccg ggggatcacg ctgtgccagg aggcgtacca
    15181 gcggcagccg ccaacccgct ccagcagttc atacacgccg tacagcgtgc cgcggaccgg
    15241 accgccgaga atcagcagat gcggcggacg ggtcacgatg cggaacccgt cgtcgccgaa
    15301 cgcggccgca tcggcggcag tgccgagaat cgcggcggtg tggcgcgtga cgcccagcag
    15361 caccgcgcgc gccggcaacg gttcccggtc cgtcacgatc ggcagacgca caccggtcat
    15421 ctgttcggtg aaccgctgaa gctcttccgc ggcgtggcgt tgcgacgccg acgcctcctc
    15481 ggaccggacg atcgccatgc cgtccgcctt tccgcgctcg gcgagcgtca cctcgcccgg
    15541 cgcccccagt acgcttgccg ccgcacatcc cgtcaacccg acgatccaaa ggattcctct
    15601 gcgcatcatg tcacccccct ttcgttcttt acgccccctg ttttacccat gtgcccgatc
    15661 aacaaggcac cgcctctcct ccgcccgcgt tccctgatct cctgaaaagt cgtggcaggg
    15721 cgttcgggcg cggaacagtt gtcctttcac gcaggagtat accggttcgg ccgcttccgg
    15781 acaatacccc ctgccgcttc agcgtaatcg gttagcgaca ggagtgtgcg aagacaatgc
    15841 gtttctggtg aaaagggggg gcagtgagtc gggagcgcgg gcgtccccgc ccgcaacagc
    15901 gtgtcttgcg ttttctggtt ttatccacag atttgcacag attgtttgtt tttatgtttt
    15961 tgaccgtaag agggagggac gagcgtctgc ccgtccgggc ggcgtttcac gccgccgcct
    16021 ttgaacgccc aacgcccaac gccgaactca gaacgtcgaa gtgaaaaaac tcctaactcc
    16081 ttgcctataa attgacagga
//
LOCUS       DAOSRB010000047        15411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3942_length_15411_cov_6.716332, whole genome shotgun sequence.
ACCESSION   DAOSRB010000047 DAOSRB010000000
VERSION     DAOSRB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15411
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_15411_cov_6.716332"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..984)
                     /locus_tag="PLZ74_04145"
     CDS             complement(334..984)
                     /locus_tag="PLZ74_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR97545.1"
                     /translation="MKTNDPVSETASAAIRPTIFLVDDDPSVRKALPRLLQAAGYEVI
                     TFASAQEFLDRSRFDGDGCLVLDVRMTGMDGLALQEELAKDDSPLPIVFITGHGDVPM
                     SVRAMKRGATDFLTKPIDQTDLLQAVQAAIQKNGVRRKQAEERQQACDCIATLTPRER
                     DVMKLVVAGLLNKQIAQKLGICEATVKVHRRRVMKKMGVASVAGLVKICFLCDHAG"
     gene            complement(981..2510)
                     /locus_tag="PLZ74_04150"
     CDS             complement(981..2510)
                     /locus_tag="PLZ74_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR97546.1"
                     /translation="MGDDVRSGNGDGFVFATLRGAVVSRASASLTTRLGYAVEEVENR
                     SLKNFVHADDWPEMKRFLKRGAGTGGAQTEIGYRIRHKDGSWRYFVSTLVPSEDGIGE
                     PGTVLFVSRDVTKRKRLEDQIIQDQTLLKTVLDVVPVIICVRNKEGRYLLVNKQGENY
                     FGKPASSIIGKLAGEISPNTEKVAAYLVWEGEVIASGKPKYISEEILKNHVGEIHVFD
                     GLMVPIFVNNEQAVLFMARDNTERYNMERERRELRENLYRADRLGRGGEIAMALTHEI
                     SQPLTGILSNAQATQLMLAQKEPDLTEIANILADIVADVKHAGNVIKRLSSYLKKKKP
                     VLDRIDVNEAILEIIKLLRSDSELKNVAVSTELAANLPRIMADRIQIQQVVLNLIMNA
                     GYAMAEQPTDQRRITVKTFLDGMKAVMVSVCDTGPGIAQKNLPRLFDAHFTTKANGMG
                     IGLAICHSILEAHDGRIWAENNPHGGATFMFSLPSTKKNRRYVKNGNVAVRGSFGGGV
                     P"
     gene            3061..3456
                     /locus_tag="PLZ74_04155"
     CDS             3061..3456
                     /locus_tag="PLZ74_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR97547.1"
                     /translation="MAKILIVDDERTILLTIQHLLNEQGFETCEASNGLAALQLFQTQ
                     FFDLVISDLRMPRMDGVSFLHEVKNLDPTVPIIVLTAYATSEILSAANERHAFTYLPK
                     PFQSETLLRIVGELLSAAPRGTDQAAVRA"
     gene            3530..4168
                     /locus_tag="PLZ74_04160"
     CDS             3530..4168
                     /locus_tag="PLZ74_04160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR97548.1"
                     /translation="MHVRHIIWDWNGTLLDDTRAGLQAVNAMLEHRGLPALDLTTYRE
                     IFGFPVRTFYRSAGFKLEEEDWDALAREFHDYFQADTTLRLHAHAVEVLEHIRRLGIG
                     QSVLSASEQTLLRTMLHDFGVTRFFEGVFGVDNLYGHSKLELGRELLARLALHPSAVL
                     LVGDSLHDHEVAADLGVACVLISQGHQSAARLARSGAPILHSLAELPVWLED"
     gene            4245..5186
                     /gene="cysK"
                     /locus_tag="PLZ74_04165"
     CDS             4245..5186
                     /gene="cysK"
                     /locus_tag="PLZ74_04165"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOR97549.1"
                     /translation="MPYFTDNSLSIGNTPLVRLHTLTQGLRATVLAKVEGRNPSYSVK
                     CRTASSMIWEAERDGLLKPGMGIVEPTSGNTGIALAMVGAARRYPVTLTMPENMSTER
                     RRILAAFGARLDLTPATAGMAGALQRAQELFNDSSGKLFMPNQFANPANPLIHERTTG
                     PEIWSQTEGQVAAVIAGVGTGGTLIGTGRYFRNIGAEVSMVAVEPDESPLLTQRAAGA
                     PLNPSGHGIQGIGANFIPQIVDFSLIGRIETVTTQEAMETARRLACEEGLFSGISSGA
                     AVAAALRLAADDAFAGKQLVVILPDTGERYLSTSLFT"
     gene            5204..6460
                     /locus_tag="PLZ74_04170"
     CDS             5204..6460
                     /locus_tag="PLZ74_04170"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOR97550.1"
                     /translation="MTVAFKTFGCRLNQAETAQYAAAFAAAGIANVPFGMPADIVVVH
                     TCTVTRTAEDTCLKQIRQLRRNAPEALLVVSGCAVETVAAQTFDALTVDLVIPREHKD
                     ELVARVLRHLGIDTPRPPVPCRPLAHRATLKIQDGCDFACAYCIIPHTRGKPQSRPFD
                     ACVQEARERIAAGFAEIVVTGCNIACYADAGRRLPDLLTALATLPGLGRLRIGSIEPG
                     TVEREVIAQMAECPTICRFLHLPIQSGDNAVLARMRRRYTIEEVAATVSDAAQRMPDL
                     GLGADLIAGFPGETPERFERTKHWVSQLPFNNLHVFPFSERPGTAAATFDGTVPPAER
                     KRRVRELNRIRTRLREAFAEQWVGRTVRVLIERIDSAGRGSGWTGEYLACRVEGLPAS
                     AEGRLVSCVPYARDADLLLARSLQQS"
     gene            6591..7526
                     /locus_tag="PLZ74_04175"
     CDS             6591..7526
                     /locus_tag="PLZ74_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOR97551.1"
                     /translation="MSKPDPSPKPGLSLTSFFSADDILTHLEQLTRNDCIRRILTHLS
                     DVHGFNDTDAYYDAILEREDATHTVVANGIAIPHARIMDLQRPYVGIATSEQGIPFAE
                     DEPPVHLILLVLIPRDQPGLYLQILRALASILRDRTAAKAVSRLRTPDEVMRFFERGG
                     MVLPDFVCAADIMEDTFISLRDNDNLKTCIDCFISQQINEIPVLDRDGDMVGVVRAGA
                     LLKVCLPEYLLWMSDLSPIVNFEPFTTVLRNEQHSCLSDILLQNFASVQMKAPAISVA
                     GEMIRHNASRCYVLNGKKLIGVITLSRFLSKIFRE"
     gene            7537..9045
                     /locus_tag="PLZ74_04180"
     CDS             7537..9045
                     /locus_tag="PLZ74_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOR97552.1"
                     /translation="MYSEPVVTLPAPRILIKIGFMAAITALAGWGAHLIGLAPQPLAV
                     VAVCTFVSVICTTLFFWTHRVAVAFLGVAVLIGSRAMTLHGMLRATELDIIFFLVGMM
                     VIVGALKDLGFLTWIIQSILNRKRMTGTSFTVILCFLSALLSSVVDEVSSIVVVLALV
                     FQVCDTLKIKPTPFVLIAVLCTNIGSAATMLGNPVGIFIGNKAGFNFSQFLAGASPIA
                     FVSLCATLCVVLLWFRKPIHEMTEKMDEHRTAHHGLAPMIRIPYRRGLFVLLATVAII
                     AFHHQIENILGLTGTENKNAFLIITPLIIAGLLMIYRPQQARHYIEHDVEWWTLLFFM
                     LLFSIAGSLEEQGITRNLALRLSEQVNGGPRAMTPLVLLISSLGSAFVDNIVFVAAFT
                     PVIQTLVERSAEFSILWWALLFGACFGGNITAIGSTANIVALGLLEKRGHQHVAFFEW
                     LKIGVLVGLITGLIAWGMLSVMTMPQPVRQPARESVVEGPLHEPHLSPVPTS"
     gene            9079..12243
                     /locus_tag="PLZ74_04185"
     CDS             9079..12243
                     /locus_tag="PLZ74_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOR97553.1"
                     /translation="MIAWGGPEVYAQAERLAKRGAVLQAELCGDSVSGVIAREGASEI
                     RTRFRLLANGLIESQCPCPTHVRSGLVCPHVVALGITLMLRKTDPQREAKYREDLRRA
                     RRIAQIPPENYLQRGADGLPARIVLTLSPDWVSEFRNAEVGVRLQLDLPSGLTDPAAV
                     PRGSKLALPAADDILLTVIEDIVGGPPPERFTVDAADFLNLLDVASQAILRTAGSETL
                     TVEQIPMKTLLKVDLDPDSGDLIVYPFAELPFLQTGSLPVFLVYGRRGRILANGHLWP
                     MENVLPIPYHSIYCRDEVITRDRVLTFLHRDLPALEAHTPVMMEVPPDLFVTEPGEPV
                     FRLTLQGSQASLRATLSACYGPHTLPAGSPETQGELALPDPADILKYRTRNSAAEAAA
                     CRLLTAYGFEGPPPEYTLVGTRPVLNFLGTGYRSLGRAGWKITFTGKLSSLIDTLPVI
                     TPVVRINETASGWFDVGFDFDRQNGPLLPPAEIQRALNRGDAFLEHHGTTLLIDREAV
                     DAMRAVFEDCPSRESPVPGHFLLPPVYAPFVHASLNALDGIDVEDPPDWRDRASARNR
                     DAHTRLTPIPLGMLENTLRPYQKEGVYWLRFLEEAGFNGILADEMGLGKTVQTLAWLS
                     LQRSDAETRGKTALIVCPTSLVENWNREAKTFVPHLRRVILSGPDRATLFPRVAEYDL
                     AITSYALLRRDLEAYRALRFAVVVLDEAQHIKNRSTQNAVAAKQIHAVGKLVLTGTPV
                     ENSVADLWSIMDFLMPGYLGEYEGFRLGYELPIAAGGREGELAQAKLRRKLHPFLLRR
                     LKRDVAHDLPDKIVRVSYCRLTSDQQKVYSDLLAVSRRSIGDLVRTKGFDRSRMEILA
                     MLMRLRQACCHLDLLKDHHPPGSYAAPSSKFEAFFELLDEAMDGGHRILVFSQFVTLL
                     KLLRAELDQRGIPCCYLDGATQDRLAQCQRFNLTPSIPVFLISLKAGGTGLNLTGADM
                     VIHLDPWWNPAVEDQATDRAHRIGQKRTVYSIKLIAEHTVEEKVLALQRKKQAVIQAT
                     LGTTDAALMRTLSFEDIRDLVGLT"
     gene            12694..13953
                     /locus_tag="PLZ74_04190"
     CDS             12694..13953
                     /locus_tag="PLZ74_04190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOR97554.1"
                     /translation="MSIASPCRAQAESPYPIVWRDLTQIQNALGTGDPHWDGESLLFS
                     NEQHQIRFFPGRRKADVNGTTVWLNAPATGSIAARDWRLAGTDLDLLQISLLPTPEAD
                     SVPEPLRVMLDPGHGGDDTGASCGNPAVREKDLTLAIAKRVGELLKRSGMRVAYTRTR
                     DVTLSLDERVRLARKHRPDLFVSLHANHAANTNATGVETYVLPPSGFPGTAEGSPARG
                     WQIGNRNDYHNTLLGFAIHRRLAALTNTVDRGLKRQAFFVLRENGCPATLLEFGFLSN
                     RDETLLMKTEPWQAACAEAIVEGILGYARRVTALDRAVAEKRARDAELNERWRRHLAE
                     RDRASQPAPTEAAPPPAARETVPEVPPSASLSAPPPRRNTDTGLSDARIARVSGTAPA
                     VPILRGTNVAPAELNTLIDFYGAGTVE"
     gene            13959..>15411
                     /locus_tag="PLZ74_04195"
     CDS             13959..>15411
                     /locus_tag="PLZ74_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97555.1"
                     /translation="MQNPPDPVPTETGSNHTPPTHLAHTLSLAAALVLIPALLGVLAM
                     LAFQKFNPRPRPPAAGVAEEMPFTGGTDTVERLTLWRRDLRHEGLPIEEANLRIWAAR
                     ATEPEILFRIAPPAPNQSWLWFVTQDGLHALAVSRQIDVLERHTVGLCDLITTQWVWT
                     VSMRWPECYAQPAVIGGHTLVRYAANGQRFAMELSPKGQILNLDALGQGMVEVWQPPP
                     PQTRFPGTPVAVTRNVFFAADEESGSLLGYAIESLPGLRYAGKGDAHTVCSGNGLLIF
                     RAREGRITVTDALTQTVLAEYNAWRHTPDTAVTATLASPDGSQFTVFLLTRFPGEPPV
                     EREWCVILDLPEGRARPFFHADALSSRPQPPADPLQTISPDGRWCLSLTEGNVLRIAA
                     GAERREWAHCALAPLTGVRQPFDALTFLDEGRHVLLRQRDNLWLLDFATARQYADLLA
                     RIHASDAAVASGAVAEPVTNAYGTFPMLPNRDAT"
BASE COUNT         2921 a         5161 c         4274 g         3055 t
ORIGIN      
        1 cgcatcgtcc gccacctgcg cgatcagagc cttttcgagc ttcgttttgt atgcggagcc
       61 cgccgccgca tgtgatgccg caggcagtcc cgccacgacc cccgtcacgg cggcggcctt
      121 cagaaagtca cgtcggttca tttgctttcc tttaccttcg tcgttttcac tctcgacaca
      181 tggtctacgc ccatttagca aggccagtat aacacagaca gaccccgcga acccctcaaa
      241 atatgcaaaa agattgtcgc gtctaagatg tcggggcttg cagccgaaag ctcggcgata
      301 gtccgggaca ttgcgtcgga tgagaacccc ggttcaaccg gcgtgatcgc atagaaaaca
      361 gattttaacc agtcctgcca ctgaggcgac acccattttc ttcatcactc ggcggcgatg
      421 caccttgacg gtcgcctcgc agatgcccag tttttgggca atctgcttgt tgagcaggcc
      481 agccacaacc agtttcatca cgtcgcgctc gcgcggggtc agagtggcga tacagtcgca
      541 tgcctgttgc cgttcttccg cctgcttgcg ccggacacca ttcttttgaa tggccgcttg
      601 aaccgcttgc agcaaatccg tctggtcgat cggttttgtc agaaaatcag tggcaccccg
      661 cttcatcgcc cgcacactca tgggaacatc gccatgcccc gtgataaaaa cgatgggcag
      721 aggcgaatca tctttggcga gttcctcctg aagcgccaag ccgtccattc cagtcatccg
      781 aacatcgagc accaggcacc cgtccccatc aaaccgcgac cggtctaaga actcttgggc
      841 cgatgcgaag gttattacct catagcccgc ggcttgcaac aggcgcggca gcgccttgcg
      901 caccgagggg tcgtcgtcaa ccagaaatat cgtcggccgg atcgccgcgg atgcggtttc
      961 ggatacagga tcgttcgttt tcatgggaca cctcctccaa aagagcccct tactgcaacg
     1021 ttaccgtttt tcacataccg tcggtttttt ttcgtgctcg gcagcgaaaa catgaacgtg
     1081 gcgccgccgt gcggattgtt ctccgcccat atccgtccgt cgtgcgcctc cagaatagag
     1141 tggcaaatcg caagcccgat gcccattccg ttcgccttcg tcgtgaagtg cgcatcgaac
     1201 aaccggggca ggttcttctg agcaatcccc ggtcccgtat cgcacaccga caccatcacc
     1261 gctttcatgc catccagaaa cgtcttcacc gtgatgcgcc gctgatccgt cggttgctcc
     1321 gccatggcgt acccggcatt catgatcagg tttagcacga cttgctgaat ctggatccga
     1381 tccgccatga ttcttgggag attcgcggcc aactccgttg acaccgcgac atttttcaat
     1441 tcggaatcgg accgaagcaa tttgataatt tcaaggatcg cctcattgac atcgatccga
     1501 tccagtacgg gcttcttctt tttcaaatac gaactcaacc ttttaatcac atttccggcg
     1561 tgtttaacgt ctgccacaat gtcagccaga atgttcgcaa tttccgtcaa atccggctct
     1621 ttctgcgcca gcataagctg ggttgcctgc gcgttactca agatacccgt caacggctgg
     1681 ctgatctcgt gtgtcagggc catagcgatc tcgccgcccc gccccaaacg gtccgcacga
     1741 tacagattct cgcgaagctc gcgcctttcc cgttccatat tgtagcgctc ggtgttgtca
     1801 cgtgccatga acaaaacagc ctgctcgtta ttgacgaaga taggcaccat caaaccatca
     1861 aacacgtgaa tttcgccgac gtgatttttc aagatttctt cagagatata tttcggtttt
     1921 ccggacgcga tgacctcgcc ttcccagaca agataggcag ccactttttc agtgttggga
     1981 ctaatttccc cggccaactt cccgataatt gaagatgcgg gtttcccaaa gtaattttct
     2041 ccctgcttgt tcaccaacaa gtaacggcct tccttgttcc taacgcaaat gatgacaggc
     2101 acgacatcca gaacagtctt cagtaatgtc tgatcctgaa tgatttggtc ttccagcctc
     2161 tttcgtttcg tgacgtctct ggaaacgaac aacacggttc caggttcacc tattccatcc
     2221 tctgaaggca ccagcgtcga cacaaaatat cgccagcttc cgtccttgtg gcggatccga
     2281 tacccgattt cggtttgggc tccccccgtt ccagcgcccc gttttaaaaa ccgcttcatt
     2341 tccggccagt cgtcggcgtg tacaaaattc ttgagtgatc ggttttccac ctcctcgacc
     2401 gcatagccta aacgggtggt caaggacgcc gaagcccggg acacaacagc gccccgcagc
     2461 gtggcaaaca cgaatccgtc gccgttacct gatcgaacgt cgtcccccac aaaaacctcc
     2521 ctagcgattc actccgaccg aaaccgatga ccacccggca caagccgggc ggtctcgttc
     2581 tcgacctcgc tcacggtcgg cggaaaggtt ccttgaaagt attaccatcc cccaagacag
     2641 gccagaaaca ttccggttcc aaagattggc atctatcatg caagggaagg gtgaaaaccg
     2701 tcaaccccta aaatttctca agacgtgtca tccgaaaagg gacgggcgat cggcacctta
     2761 ccctgagacc cggttcgctg aattctcaag cgtgtgcctc gcacaccccc gttcttttct
     2821 aaaaaatgac aaaaccgggc ccgccccgca tcccccggca tccgctcccg aaacttttgt
     2881 gcttcacgag gcccgaactc gaaaacgccg ttatttcgtt cattttgcat ccctcctccg
     2941 catgcgtcgc gccgccggtc ctccgggaag ggatcgaaat ggcacacctt ttgctagaat
     3001 catttgcgta tttgcgtatc gaccctgaca aaccgatgat gtcttgggga accgcttgca
     3061 atggctaaaa tcctgatagt cgacgatgag cgaacgattc tgctgacgat tcagcatctt
     3121 ttgaatgaac agggatttga aacgtgcgag gcatccaacg ggttggccgc actccagctc
     3181 ttccagaccc aattcttcga tctggtgatc tcggacctga gaatgccgcg catggacggc
     3241 gtctcctttc tgcatgaagt caaaaacctc gacccgaccg tacccatcat tgttttgacg
     3301 gcatacgcca cgtccgaaat cctttccgca gccaacgagc ggcacgcttt cacctatctg
     3361 cccaagccct ttcagtccga aaccctgctc cggatcgtgg gcgaattgct gtccgccgca
     3421 ccccggggca cggatcaggc ggcggtgcgg gcctgaatcg gacccgcggt tcacaggacg
     3481 ggtgacccgc gacgccaccg ggaaggcgat aaaggaaccg tctgcaagta tgcatgttcg
     3541 ccatattatt tgggattgga acggaaccct gctcgatgac acccgggcgg gtctccaagc
     3601 cgtgaacgcc atgctcgaac accgcgggct gcctgcgctg gatctcacga cctaccggga
     3661 gattttcggc tttccggtcc ggaccttcta ccggtctgcg ggatttaaat tggaggagga
     3721 ggactgggac gccctggccc gtgaattcca cgactatttt caggccgaca ccacgctgcg
     3781 gctgcatgct cacgcagttg aggtcctgga gcacatccgg cgcctcggaa tcggccagtc
     3841 cgtgctctcg gcttcagaac agaccctcct ccgcaccatg ctgcacgatt tcggcgtgac
     3901 ccgttttttt gaaggcgtct tcggggtaga caatctgtac gggcactcca aactggaact
     3961 ggggcgcgaa ctgctggcac ggctcgcgct gcacccgtcg gcggtgctgc tggtcggcga
     4021 ctccctgcac gatcacgagg tagcggcgga tctgggcgtg gcctgcgtcc tgatctccca
     4081 gggccaccag tccgccgcgc gcttggcccg cagcggcgct ccaattctgc acagtctggc
     4141 ggaactcccc gtctggttgg aagactgaac cgatgcgtaa aatgctttgt cctttgtcgc
     4201 cgcaggggaa cctgttaaag tgattggatt cagaagaggg tgccatgcca tatttcactg
     4261 acaattcgct gtcgatcggg aacacaccgc tcgtgcggct ccatacgctc acacagggat
     4321 tgcgggccac tgtgctcgcc aaagtggagg ggcgcaaccc ctcctattcc gtcaaatgcc
     4381 ggaccgcctc atccatgatt tgggaggccg aacgggacgg cctgctcaag cccggcatgg
     4441 ggatcgtcga acccaccagc ggcaacacag gcatcgccct cgccatggtg ggcgccgcgc
     4501 ggcgttatcc ggtcacgctg accatgccgg aaaacatgag tacggaacgc cggcgcatcc
     4561 tcgccgcgtt cggcgcgcgc ctggatctca cgcctgcaac cgcaggcatg gccggagcgc
     4621 tccaacgggc acaggaactg ttcaacgact cctccggaaa actcttcatg cccaaccagt
     4681 tcgccaaccc cgccaacccg ctcatccatg aacggaccac cggaccggaa atctggagtc
     4741 agaccgaggg ccaggtcgcc gctgtgattg cgggggtggg taccggcgga accctgatcg
     4801 gcaccggaag gtatttccgg aacatcggcg cggaggtgag catggtggcg gtcgagccgg
     4861 atgaaagtcc cctgctgacg caacgtgccg caggggcacc cctcaatccg tcgggccacg
     4921 gcattcaggg gatcggcgcc aatttcatcc cgcagatcgt ggacttctcg ctgatcggcc
     4981 ggatcgaaac cgtgaccacg caggaagcaa tggaaaccgc acggcgtctg gcatgcgaag
     5041 aaggcctctt ctcgggcatt tcaagcggcg cggcggtcgc cgcggccctg cgcctggccg
     5101 cagacgatgc gtttgcggga aaacagttgg tggtcatcct gccggacacg ggcgagcgtt
     5161 acctctccac gtccctcttc acctgaccgc attgccgaag ctcatgacgg ttgccttcaa
     5221 gaccttcgga tgccggctga atcaggccga aaccgcacag tatgcggccg cattcgcggc
     5281 agcgggcatc gcgaatgtgc cgttcggcat gcccgccgac attgtggttg tccatacctg
     5341 cacggtcacg cgcacagcgg aagacacctg cctgaagcag atccggcaac tgcggcggaa
     5401 tgcgcccgaa gcattgctcg tggtctcggg atgcgcagtg gaaaccgttg cggcgcaaac
     5461 gttcgacgcg ctgaccgtcg acctcgtgat accgcgtgaa cacaaagatg aactggtggc
     5521 gcgtgtgctg cggcaccttg gcatcgacac cccccgtccg ccggtaccct gccgacctct
     5581 cgcccaccgc gccaccctga aaatccagga cgggtgcgat ttcgcctgcg cctactgcat
     5641 cattccccac acgcggggta aaccgcaaag ccgccctttt gacgcatgcg tgcaagaggc
     5701 gcgggaacgg atcgcggcgg gtttcgccga aatcgtggtg accggatgca acatcgcctg
     5761 ctatgcggac gccggacgcc gtctaccgga cctgctgacc gcactcgcca ccctgccggg
     5821 cctcggccgc cttcgtatcg gctcgatcga acccggcacc gtcgaacgcg aggtgatcgc
     5881 gcagatggcg gaatgtccca ccatctgccg cttcctgcac ctgcccatcc agagcggcga
     5941 taacgccgtc ctcgcgcgca tgcggcgacg gtatacgatc gaggaggttg ccgcaaccgt
     6001 ttccgatgcg gctcaacgca tgcccgatct cggtctcggc gcggacctga tcgccgggtt
     6061 tccgggcgag accccagaga ggttcgagcg cacgaaacac tgggtttccc aacttccgtt
     6121 caacaacctg cacgtctttc cgttcagtga gcgtcccggc acggcggccg ccacattcga
     6181 cggcaccgtc ccgccggccg aacgcaaacg gcgcgtacgg gaactgaacc gtatccggac
     6241 ccggttgcgc gaggccttcg ctgaacaatg ggtcggacga actgtccgcg tgctgatcga
     6301 acgcatcgac agcgcgggcc gcggaagcgg atggaccggc gaataccttg cctgccgcgt
     6361 ggagggcctg cccgcatcgg cggaaggccg gctggtatcc tgcgtcccgt acgcgcggga
     6421 cgcagatctt ctgctggccc ggtccctgca acaaagttga atcgtatcca aaggaaaggt
     6481 tctgcgttgt ttttccgcaa ccgaaacggt atagtcatcg ctgttcggtc cggaatcgcg
     6541 gcagacgcaa aagaaggacc cacccgacca gcggtgaacg gaacgtagac atgagcaaac
     6601 ctgacccatc acccaaaccc ggcctgtccc tcacgtcgtt cttcagtgcg gatgacattc
     6661 tgacgcatct ggagcagctc acccgcaatg actgcatccg ccgcattctc acgcatctct
     6721 ccgatgtgca cgggtttaac gatacggatg cctattacga tgcaatcctc gagcgcgagg
     6781 atgccaccca cacggtggtg gcgaacggaa tcgccattcc gcatgcgcgc atcatggatc
     6841 tgcagcggcc ctatgtcggc atcgcgacct ctgaacaggg catccccttt gcggaagatg
     6901 agccgcccgt gcatctgatc ctgctggtgc tgatcccgcg tgaccagccc ggactctatc
     6961 tccagatcct gcgcgcgctc gccagcatcc tgcgcgaccg caccgcggcg aaagcggttt
     7021 cacgcctgcg cacgccggat gaggtcatgc ggttcttcga gcggggcggc atggtgctgc
     7081 cggattttgt gtgcgccgcg gacatcatgg aggacacctt catctccctg cgcgacaatg
     7141 acaacctgaa aacctgtatc gactgtttca tatcgcagca gatcaacgag attccggtgc
     7201 tcgaccggga cggggacatg gtcggtgtgg tgcgggccgg cgccctgctc aaggtctgcc
     7261 tgcccgaata ccttctttgg atgtcggatc tctcccccat cgtcaatttc gaaccgttca
     7321 ccaccgtgct gcgcaacgaa cagcactctt gtctctccga catcctgctg cagaatttcg
     7381 cctcggtgca gatgaaggcc cccgccattt cggtcgccgg cgagatgatc cgccacaatg
     7441 cctcccgctg ttacgtgctg aacggcaaga agctgatcgg cgtcatcacc ctgtcgcgtt
     7501 ttctgagtaa aattttccgg gagtgagttt tccgatatgt acagcgaacc cgtcgtcacc
     7561 ttacccgcac cccgcattct gatcaagatc ggtttcatgg ccgccattac cgcgttagcc
     7621 ggttggggcg cccatctgat cggactggcg ccccaaccgc tggccgtggt cgcggtctgc
     7681 acgttcgtca gcgtcatctg caccacactg ttcttctgga cccaccgtgt cgccgtggcc
     7741 tttctcggcg tcgccgtgct gatcggatcg cgcgccatga cgctgcacgg catgctgcgc
     7801 gccaccgaac tcgacatcat ttttttcctc gtcggcatga tggtgatcgt cggcgccctc
     7861 aaagacctcg ggttcctcac ctggatcatc caatccatcc tcaaccggaa acgcatgacc
     7921 ggcacctcct tcaccgtgat cctctgcttt ctctcggccc tgctctccag tgtggtggac
     7981 gaggtctcct cgatcgtggt ggtcctcgcg ctcgtttttc aggtttgcga caccctcaaa
     8041 atcaagccga cgccgtttgt cctgatcgcg gtcctctgca ccaatatcgg ctcggccgcc
     8101 accatgctcg ggaaccccgt cggcattttc atcggcaaca aggccggatt caacttcagc
     8161 caattcttgg cgggcgcctc cccgatcgcg ttcgtctcct tgtgcgccac cctgtgcgtg
     8221 gtgctgctgt ggttccgtaa accgatccac gagatgacgg aaaagatgga cgagcatcgc
     8281 accgcccatc acgggctggc ccccatgatc cgtatcccgt accgccgggg gctgtttgtc
     8341 ctgctcgcca cggtcgccat catcgcgttc caccatcaga tcgaaaacat tctcggcctg
     8401 accggcacgg aaaacaaaaa cgccttcctg atcatcacgc cgctcatcat cgccggcctg
     8461 ctcatgatct accggccgca acaggcgcgc cactacattg aacacgatgt ggagtggtgg
     8521 accctgctct tcttcatgct gctcttttcc atcgcgggct cgctggaaga gcaaggcatc
     8581 acccgcaacc tggccctccg cctcagtgaa caggtcaacg gcggaccacg cgccatgacg
     8641 ccgctcgtgc tgttgatctc ctccctcggt tcggcatttg tcgacaacat cgttttcgtg
     8701 gccgccttca ctcctgtgat ccaaaccctg gtcgaacgtt ccgccgagtt ctcgattctc
     8761 tggtgggcgc tgcttttcgg cgcctgcttc ggcggcaaca tcaccgccat cggctccaca
     8821 gcgaacattg tcgcgctcgg cctgctggaa aaacgcggac accagcacgt cgcctttttc
     8881 gaatggctca aaatcggtgt gctggtcgga ttgatcacgg ggctgatcgc ctggggcatg
     8941 ctgagtgtca tgaccatgcc gcaacccgta cgccagccgg cccgggagtc cgtggtggaa
     9001 ggtccgttgc atgagccgca cctctccccc gtcccgacat cctgacgtat gccgctttcc
     9061 gccataaccc ggaaacaaat gatcgcgtgg ggcggtccgg aggtgtatgc gcaagcggaa
     9121 aggctggcaa aacgcggcgc ggtgctccaa gccgaacttt gcggcgactc tgtttccggt
     9181 gtgatcgcgc gcgaaggcgc gtccgagatc cgtacccgct tccgcctctt ggcgaacggg
     9241 ctgatcgaaa gccagtgccc ctgccccacc catgtccgca gcggcttggt ttgcccgcac
     9301 gtcgtggccc tcggcatcac cctgatgctg cgcaagaccg atccgcaacg cgaggcgaaa
     9361 taccgggagg acctgcggcg cgcccggcgc atcgcccaaa tccccccgga gaattacctg
     9421 caacggggcg cggacggtct tcccgcccgg atcgtgctca ccctctctcc agactgggtc
     9481 tccgaattcc gtaacgcgga ggtgggggtc cggctgcaac tcgatctgcc ttccggcctc
     9541 accgacccgg ccgctgtccc gcgcggttca aagctcgcgc tcccggccgc ggatgacatc
     9601 ctgcttaccg tgatcgagga catcgtcggc ggtcccccgc ccgagcgctt cacagtggat
     9661 gccgccgatt ttctgaatct tctggatgtc gcctcgcagg cgattctccg cacagccgga
     9721 tccgaaaccc tgacggtcga gcagatcccc atgaagaccc tgctcaaagt ggatctggat
     9781 ccggattcgg gcgacttgat cgtctacccc ttcgcggaac ttccgttcct ccaaaccggc
     9841 agcctgcccg tcttcctcgt gtacggacgg cgcggccgca ttctcgccaa cggtcacctg
     9901 tggcccatgg aaaacgtcct gcccatcccg taccacagca tctattgccg ggacgaggtc
     9961 atcacgcggg atcgcgtcct gacctttctg caccgtgatc tgcccgctct cgaagcgcac
    10021 acgccggtga tgatggaggt gccgcccgac ctgttcgtga ccgagcccgg cgaaccggtc
    10081 ttccggctca cgctgcaagg ctcccaagcc tcgctccggg ccacgctctc cgcctgctac
    10141 ggccctcaca ccctccctgc cggatccccg gaaacgcaag gggaactcgc attgccggat
    10201 cctgcggaca tccttaagta ccgtacacgc aactccgccg ccgaagccgc tgcctgccgc
    10261 cttctgacag cgtacggttt tgagggcccc ccccccgaat acacgctggt cggcacacgg
    10321 ccggtactca atttcctcgg caccggttac cggtctctgg gccgcgccgg atggaaaatc
    10381 accttcaccg gaaaactgtc ctcgctcatc gacaccctgc cggtcatcac tccggtggtc
    10441 cggatcaacg agaccgcctc cggctggttt gacgtggggt tcgattttga ccggcagaac
    10501 ggcccgctac tgccacccgc cgaaattcag cgcgccctca accgcggcga cgcgtttctc
    10561 gaacatcacg gcaccaccct gctgatcgac cgggaggccg tagacgccat gcgcgccgtg
    10621 ttcgaggact gcccgagccg tgaaagcccc gttcccggcc actttctgtt gcctccggtc
    10681 tacgcccctt tcgtgcatgc ctcgctcaac gcgctcgacg ggattgacgt agaagacccg
    10741 ccggactggc gtgatcgcgc cagcgcacgc aaccgcgacg cgcacacacg gctcaccccg
    10801 atcccgctcg gcatgctgga aaacactctc cgcccctacc agaaggaggg cgtgtactgg
    10861 ttgcggtttc tggaggaagc cggatttaac ggcattctgg ccgacgagat ggggctcggc
    10921 aaaacggtgc agaccctcgc ctggctgagc ttgcagcgaa gcgacgccga aaccagaggg
    10981 aaaaccgcgc tgatcgtctg ccccaccagc ctggtcgaaa actggaaccg tgaggcgaag
    11041 acctttgtcc cccatctgcg ccgtgtgatc ctctccggtc ctgaccgggc cacgctcttt
    11101 ccgcgtgtgg ccgaatacga cctggcgatc acctcgtacg cgctcctgcg ccgcgatctc
    11161 gaagcctacc gcgccttgcg gttcgctgtt gtcgtgctgg atgaagcgca gcacattaaa
    11221 aaccgttcga cacaaaacgc cgtggcagcc aaacagattc atgccgtcgg caagctggtg
    11281 ctcaccggca caccggtgga aaacagcgtg gccgacctct ggtcgatcat ggattttctg
    11341 atgccagggt acttgggtga gtacgaggga ttccgcctcg gctatgaact gcccatcgcg
    11401 gccggcgggc gcgagggtga actcgcgcaa gccaaattgc gccgcaagct ccatcccttt
    11461 ttgctgcgcc gcctcaaacg ggatgtggcc cacgacctgc ccgacaaaat cgtccgggtt
    11521 tcgtattgtc ggctcacctc cgaccagcag aaggtgtatt cggatctgct cgccgtatca
    11581 cgccgctcca tcggcgatct ggtccggact aaagggttcg accgctcgcg catggagatc
    11641 cttgccatgc tcatgcgcct gcgtcaagcc tgctgccatc tggatctgct gaaagaccac
    11701 cacccgcccg gctcctacgc agcgccctca tccaaattcg aggctttttt cgaactgctc
    11761 gacgaagcga tggacggtgg ccaccgcatt ctggttttca gccagttcgt caccctgctt
    11821 aaactcctgc gggccgaatt ggatcagcgc ggcatcccct gctgttatct ggacggggcc
    11881 acacaagacc ggctggccca atgccaacgg ttcaacctga ctccctcgat tccggttttc
    11941 ctgatcagtc tgaaggccgg cggcaccgga ctcaacctca ccggcgcgga tatggtgatt
    12001 catcttgacc cctggtggaa tccggctgtg gaggatcagg ccacggaccg cgcacaccgc
    12061 atcggccaga agcgcaccgt ctacagcatc aaactcatcg cggaacacac ggtcgaagag
    12121 aaggtcctag ccttacaacg gaaaaaacag gccgtcatcc aagccaccct cggaaccacg
    12181 gacgcagcgc tgatgcggac gctctccttc gaggatatcc gcgatctggt cggcctgacc
    12241 tgaaccggtt cgccacaggg acaagtctgg agacacagat ttccccgccc gcagcagcgt
    12301 gtcttgcgct ttctggtttt atccgcagat ttttcttttt tgtctttgac cgcaggcggg
    12361 gagggacgag cctctgttcg tccgggcggc gtttcacgcc gccgcctttg aacgccgaac
    12421 gcccaacgcc gaactcagaa cgtcgaagta aaaaaaactc ctaactccta aatcctaaat
    12481 cctaactcct aaataaatcc tacatccgcg cgtagcgcgg aagacgcccg cgctcccttt
    12541 gcgcacacac aaaacacgcg ttcgactcgc atgcccctgt cacgaaccga ttgcttcaca
    12601 ccctcatttc ttccttcaac ttcggcgctc tctcccgtat actgtgtccg catcatgatt
    12661 ggttggcgtt tgtttctttt tctgttttgc tgtttgtcga ttgcaagccc gtgccgcgca
    12721 caggctgaat cgccatatcc gatcgtctgg cgcgatctga cgcagatcca gaacgcactc
    12781 gggacggggg acccgcactg ggacggcgaa tccctgctct tctcgaacga acagcaccaa
    12841 atccggttct ttccggggcg ccgcaaagcg gacgtcaacg gcacgaccgt ctggctcaat
    12901 gctccggcca ccggctcgat cgcggccaga gactggcggt tggcgggcac ggaccttgat
    12961 ctgctgcaga tctccctgtt gccaacgccc gaagccgaca gcgtgccgga acccctgcgt
    13021 gtgatgttgg atccgggaca cggcggggac gacaccggcg catcgtgcgg gaacccggcg
    13081 gtacgggaaa aggatctgac gctggcgatc gccaaacgcg tcggcgaact cctcaagcgt
    13141 tccggcatgc gggtggcgta cacgcgcacg cgggatgtca cgttgtcgtt ggatgagcgg
    13201 gtacgccttg cccgaaagca tcggcctgac ctgttcgtca gcctccatgc caaccatgcg
    13261 gccaacacca acgccacggg ggtcgagacg tatgtgctgc cgcccagcgg gtttccgggt
    13321 acggcagagg ggagcccggc gcgcggctgg cagatcggca accgtaatga ttatcacaac
    13381 accctgctgg ggtttgcaat ccaccgccgc ctcgccgctt tgaccaacac ggtggaccgc
    13441 ggcctgaaac gtcaagcctt cttcgtgctg cgcgaaaacg gttgccccgc cacgcttctg
    13501 gagttcggtt tcctttctaa ccgcgatgaa accctcctga tgaagacgga gccttggcag
    13561 gcggcgtgcg ccgaagccat cgtcgagggg attctgggtt atgcgcgacg cgtcacagcg
    13621 ctcgaccgcg ccgtggcgga aaagcgcgcg agagacgcgg aattgaacga acgctggcgc
    13681 cgccacctcg ccgaacggga cagggcatcc caaccggctc cgacagaggc ggccccgccg
    13741 cctgccgcgc gcgaaaccgt gccggaagtc ccgcccagcg cctccctgtc cgcacccccc
    13801 ccgcgccgaa acaccgatac cggtctttcc gacgcgcgca tcgcgcgcgt gagcggaacc
    13861 gccccggccg taccgatcct gcgcggcacc aatgtcgcgc ccgcggaact caacaccctg
    13921 attgattttt acggcgcggg aacagtagaa tgaatgccat gcaaaaccca cctgacccag
    13981 taccgaccga aaccggatcc aaccataccc cccccaccca cttggcacac acgctgtcgc
    14041 tcgccgcagc tctggtgctg atccccgccc ttctgggcgt tctcgccatg ctggcctttc
    14101 aaaagttcaa cccccggccc cgacctcctg ccgccggagt ggcggaggaa atgcctttca
    14161 ccggtggaac cgatacggtc gaacggctga cgctctggcg ccgcgacctg cggcatgagg
    14221 ggctgcccat cgaagaagcc aacctgcgca tctgggccgc ccgcgccacg gagcctgaga
    14281 tcctgttccg tatcgcaccg cccgcgccga atcagtcttg gctctggttc gtgacgcagg
    14341 acggtctgca cgcattggct gtttcgcgcc aaatcgacgt gctcgaacgc cataccgtgg
    14401 gcctctgtga cctgatcacc acgcaatggg tctggaccgt ctctatgcgc tggccggaat
    14461 gttacgccca gcctgcggtc atcggcggtc acacgctcgt acggtatgcg gcgaacgggc
    14521 agcggttcgc catggaactc agccccaaag gccagatcct caatctcgac gcgctcggac
    14581 aaggaatggt cgaggtctgg caaccgccgc cgccgcagac gcgttttccc ggaacgccgg
    14641 tggccgtgac ccgcaacgtc ttcttcgcgg cggacgaaga gagcggatcg ctactggggt
    14701 acgcgatcga aagtctgccg ggcctgcgtt acgcaggaaa gggcgacgcg cacaccgtct
    14761 gttccggcaa cggtctgttg attttccgtg cccgcgaggg gcgcatcacc gtgacggacg
    14821 ccctgacgca aaccgtgctg gcggagtaca acgcctggcg ccacacaccc gataccgcgg
    14881 tgaccgccac gttggcttcg ccggacggct cgcagttcac cgtgtttctc ttgacccgct
    14941 tcccgggcga gccgccggtc gagcgcgaat ggtgcgtcat cctagacctg cccgagggcc
    15001 gcgcgcgtcc tttcttccat gcggacgcgc tctcttcccg accgcaaccg cccgccgatc
    15061 cgctccagac catctcaccg gatgggcgct ggtgcctgag cctgacggag ggaaacgtgt
    15121 tgcgcatcgc cgcaggcgcg gaacgccgcg aatgggcgca ctgcgcactc gctcccctca
    15181 ccggagtccg gcagccgttt gacgcgctca cgtttctcga tgaagggcgg cacgtcctgt
    15241 tgcgccaacg cgataacctg tggctgctgg atttcgccac cgcacgtcag tatgcggatc
    15301 tgctggcgcg gatccatgcc agtgatgcgg cggttgcgtc cggcgcggtc gccgaaccgg
    15361 tcacgaacgc atacggcacg ttcccgatgt tgccgaaccg tgacgccacc c
//
LOCUS       DAOSRB010000048        15410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3943_length_15410_cov_6.968414, whole genome shotgun sequence.
ACCESSION   DAOSRB010000048 DAOSRB010000000
VERSION     DAOSRB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3943_length_15410_cov_6.968414"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLZ74_04200"
     CDS             complement(<1..340)
                     /locus_tag="PLZ74_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4964 domain-containing protein"
                     /protein_id="HOR97556.1"
                     /translation="MKAIERALFCGLVAAISIDAMAAFRPPAVPLVSVEPHFSLWSAA
                     DRLYERDTTHWAGATQSLVILLEADGITYRLCGHGPRDLVELPVLPQTRCRVGATTTY
                     YRFADGRGLSA"
     gene            431..1939
                     /gene="der"
                     /locus_tag="PLZ74_04205"
     CDS             431..1939
                     /gene="der"
                     /locus_tag="PLZ74_04205"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOR97557.1"
                     /translation="MGSVKRERGVDGPRAMLALVWARGYGYTQRMISQDQGKARVVVI
                     VGRPNVGKSAVFNRIAGRRIAIVHDESGVTRDRLMREVEWDDQRFTLIDTGGITLLTG
                     QTGKNAIEAGIRAQVDAAIGDAAVALLVVDVKQGMHPLDEEVALIVRKAGIPCVVAVN
                     KCDEPQHEAGVAEFARLGFATFPVAAQHNRGMGELMEAVLPYLPEAVNPTETQPLRVA
                     VVGRPNAGKSSYINRLLRSDRVIVSDVAGTTRDSIDVPFTIGSGAQARHYILVDTAGM
                     RNRHRIDNAVERFSLFRAEESIRGADVVVLMLDAEIGPTVQDKHIAAMIQKYNKGCVL
                     LMNKWDRAMENGMTQTRSEPVLRQIMPFMGYSPVLFISAVTGFNVRKSVETIDAVAAQ
                     TRVTLPTGILNRTLVEATERVLAPARNGKRLRVYYAVQIGVAPVSIRLFVNDPKLATK
                     PYTDFLIRSLRSRFGLEGAPVRLFYRARTRPESVERPARSRSRARGKKRKAR"
     gene            1992..3356
                     /locus_tag="PLZ74_04210"
     CDS             1992..3356
                     /locus_tag="PLZ74_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOR97558.1"
                     /translation="MAGEGLSVKTGRALVLCALTGLVAGLAASGFYWLLEAGRYLLLE
                     RLGHYSPPLAAGEFPLFPPVAHEAPVRWVLLLLPAVGGLLSALLVKGFAPSAGGHGTD
                     AAIYAYHRNEGRIPFAVIPVKALASALVIGSGGSAGCEGPVTQIGAGCGSAVAGLLKL
                     DATERRILMAAGLAAGVGAIFRAPLAGAIFAAEIFYSGLDIEYEVMMPSLVASTIAYT
                     VFALFFGWQPLFVMTPYNFDAPLKLGPYLLLALVVAFGAKLYIHVFRRTEAWCRNLSR
                     VPTWLKPALGGMLTGLIGFFLPEVLGSGYGLIQQALAVGSPLAERFGSPSFTMLWLIF
                     FGKILATSFTVGSGGSGGVFGPALVAGSTLGAATGLTLMRLLPALDLHPGAFALVGMA
                     GFLASCVRVPIAAIIMVSEITGNHELLLPSMWVCGIAYWIGNGWTLYRSQVRTRDCSP
                     AHSR"
     gene            3409..4095
                     /gene="tsaB"
                     /locus_tag="PLZ74_04215"
     CDS             3409..4095
                     /gene="tsaB"
                     /locus_tag="PLZ74_04215"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOR97559.1"
                     /translation="MRILVIDRSTSRAGMALMDAGRAICSRVWEDDAARSPGWIAEAV
                     RELAACGVGLQEVGRFVCGLGPGSFSGIRSSLAALQGMALPTGAPVCGVASAAALALA
                     YGMDVPRVTVVGDARRERLWIVTYRVEPHTGRVMLADGTRPTHTAADFTLVPASELAS
                     AIPGDAFVVSSDWARLAPRLTAACPSPRLLRETVCPDAADLGRLACADLDACVTEPLP
                     IYLHPAVVVR"
     gene            4171..4344
                     /locus_tag="PLZ74_04220"
     CDS             4171..4344
                     /locus_tag="PLZ74_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97560.1"
                     /translation="MARPTCSQRQRYTFTYTSAKHEQGADSDSDPDFDAVALQVRQMD
                     FASASGVPHAAAF"
     gene            complement(4405..5613)
                     /gene="dinB"
                     /locus_tag="PLZ74_04225"
     CDS             complement(4405..5613)
                     /gene="dinB"
                     /locus_tag="PLZ74_04225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOR97561.1"
                     /translation="MQRCILHVDMDAFFAAVEQRDHPEWRGKPVIVGAPPDRRGVVST
                     CSYEARVFGVRSAMPSREAYRRCPHAIFVPCDMGRYEEASQQVFAIFERFTPLIEPLS
                     IDEAFLDVTGGRTLFGSGEKIARAIREAIRNEVGLTASVGIAHNKFLAKLASEQAKPD
                     GLAVIPETTDAILAFLAPLPVRALWGVGRVTAQTLEKGGLRRIADIRQADPAFLASLV
                     GEHAATHLMALAHGEDARPVQTDFEEKSISREHTFPEDCRDRSVVCEVLKTLADDVGR
                     RIRLQNRYATLGKLKLRWADFRTLTRQAPFPSPVCDDFAFREQALRLFQAQPLTQPVR
                     LIGFGVSGLCNECGAEQLALFDSVPDAREKRERLCRTLDQVRERLGDHALRRVSVPEN
                     EVEKGRAGSP"
     gene            complement(5696..6262)
                     /gene="infC"
                     /locus_tag="PLZ74_04230"
     CDS             complement(5696..6262)
                     /gene="infC"
                     /locus_tag="PLZ74_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006723876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOR97562.1"
                     /translation="MNFKIRVPNVRVINANGEMLGIMATRDAQRLADQQGLDLVEVSP
                     NAEPPVCKIMDYGKFRYEESIKRKMARKNQKTVQIKEVKFHASVDSNDLAHKLRQIKE
                     FLNDGHKVKVTLQYRGRENAHKELGMEVVQKVIQECAACSVVEQNPRLIGRTLGCLLA
                     AKPAKGAASGQSAASAPQNPPPRPPAAP"
     gene            complement(6389..8134)
                     /gene="thrS"
                     /locus_tag="PLZ74_04235"
     CDS             complement(6389..8134)
                     /gene="thrS"
                     /locus_tag="PLZ74_04235"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOR97563.1"
                     /translation="MEVGVEAIDVMRHSAAHVMAHAVCNLFDNVKLDIGPSTEEGFYY
                     DFDLEHRLSPEDFPRIEAEIEALIQADLPFERLETTCGEAARILADQPYKLERLADIP
                     EGEAITLYRCGAFVDLCRGPHVNRTSEIGAVKLTSVAGSYYRGDEKNAMLQRLYGMAD
                     VSREALDAVLARMEEAKKRDHRKLGKELDLFSISEEVGPGLAHWHPKGARIRVAIEDY
                     WRREHFRAGYEMVFTPHVGRAKLWETSGHLGFYRDSMFPSMNLSEGEGSRASVESYFV
                     KPMNCPFHIQVYKTRRYSYRDLPLRWAELGTVYRYEKEGVRHGLLRVRGFTQDDAHIF
                     CTPEQIEDEVRSTVTFALRVLRRFGFREITAFLSTRPEKAVGLPERWEQATQSLENAL
                     RAEAIEYQVDAGGGAFYGPKIDLKIKDALGRPWQLGTIQFDFNLPERFALTYIGEDGK
                     EHQPYMVHRALLGSLERFFGILIEHYAGAFPVWLAPEQVRVLPITADQIAYARSLETT
                     LREADVRVTVDTDNEKLGAKIRRAQLEKVPFMLVVGKRDAEAQVVSVRSRETGDQGAM
                     TIEAFRSVLADAMNA"
     gene            8478..11888
                     /locus_tag="PLZ74_04240"
     CDS             8478..11888
                     /locus_tag="PLZ74_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR97564.1"
                     /translation="MRGRLGLAWVLAGSLALAEGVVESAKTLPLAHETDVVIVGGSSG
                     AVAAACRAAAEGVRVFVVAPRPYLGEDMAGKLCLRTGASEDPRCVLRRRMFTTTQLPP
                     SLPFSYRSDRAAASNHPDPHHRVLSDGVWQDAVTESAQFGGDVTLTLDLGALTDLGSL
                     VACVFRRDGANGFRTGALHVWGSTDAETWADLGKGIDQETEAGSERARFRVPLSGRYR
                     YVKVLALVAPGVKRQLLGELLVYPVADPSAGAGLSDRTTPLQVKKVLDEALLQAGVPF
                     LTGALATETLTDETGALAGVVIANRSGRQAIRAKVVIDATERGILARAAGGQAEPFPA
                     GHYTFTRRIVAGEAPRAEGVRVRELFGVYPVRVTGIRPPEGHPGTITGRLFECEIDLP
                     MRDGSARAFAEAEQRARDLTFVPTQLESADTLSLLPPDFIRGKAAVRAPWAGVDALDL
                     DAFQPAGTSFLFVLGPMADVARPTVEHLMRPGNLMALGERIGAAAARLAKQRPAPQGV
                     RAGGSPDAQSGGVTVGEHTRNLAAYLRNASGTVRVEPRELPVLAECDVLVAGAGTGGA
                     PAGIAAARHGARTLVCEYIHQMGGVQTDGLIGRYYFGNRVGFTLDIDRGVKETGAVFS
                     QCKSEWYRSQNRKGGAEIWYGTMVSGVVVENNRLTGVVVVMPDGTRGVVRCRVAIDST
                     GNAELAAMAGEKTEFITAAEVALQGVGQTPRRMGASYTNTDIGFLDDTDASDLCFFAL
                     RSRYSMREGIWDQAQIINSRERRRMIGAFYMSPLDVVNGRTYPDVVVQPYSNFDSHGH
                     TTHEQFFIEDPGHHGMKVNLPFRCLLPKTVDGLLVTGLGISAHRDAMPILRMQPDVQN
                     QGYAAGTAAAMAIKAGVPVRRVDIRALQRHLIEMKILPETVLDMEDSFPLPDACFTEA
                     VAAIPNGYQGLSVVLTDPARSVPLLKAAYTAARVPEARLAYAHVLGMMGHPDGETELI
                     ARVQGMAWDKGWHFRGMGQFNRSVSWLDSYLIALGRCRSARAVPAILDKAKALTAADA
                     FSHFRAVALALEAIGDPAGAPVLGRLLATEGIGGYAIRMGPELPVIPNYANHEGDRER
                     SACLRELALARALFRLGDFEGRGVRTLRAYAEDPRGAYATHAALVLGR"
     gene            12028..15123
                     /gene="secA"
                     /locus_tag="PLZ74_04245"
     CDS             12028..15123
                     /gene="secA"
                     /locus_tag="PLZ74_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOR97565.1"
                     /translation="MNLIRTLFGTKNERDIKRLQPMVTRINELEKEYQALSEQQLQAK
                     TQEFKERVAAGESLDDLLCEAFAVVKNACRRLCGTTREVCGHMLAWDMVPFDVQLIGG
                     IVLHQGKIAEMQTGEGKTLVATLPLYLNALSGNNVRLVTVNDYLARRDAQWMGHVYEY
                     LGLTVGCIQNHMGPAERREQYACDITYGTNSEFGFDYLRDNGMAVEPSQVVQRGHHFA
                     IIDEVDSILIDEARTPLIISGPVAHVSTAQYTELRPRVERLVRDQMEICNRLISEVRE
                     AIEAADSETAMFKLYQVYHGMPKHKQFLHLLEDPATRKLHEQVESMMLSEMRKELARD
                     LREQLLFTIDERTREVALTDRGSEKLSPTDPEMFVMPDLVSAMSALDGDTALGAEERA
                     KRRQQIQSDFMVRSERVHNVDQLLRAYCLYERDVDYVVQDNHVYIVDEFTGRVLPGRR
                     WSDGLHQAVEAKEGVEIERETQTLATITIQNYFRLYKKLAGMTGTAETEASEFHDIYK
                     LDVVVIPTNRPIRRVDGNDQIYKSQREKYKAILDEVEKRHRNGQPVLLGTVTVDTSEI
                     LSRMLRMAKIPHNVLNAKNHAREAEIVSLAGQPGAVTIATNMAGRGTDIKLGEGVVWV
                     PETTIRSQITLENRYDNGNKTLRKLLEEKPCGLHVIGSERHESRRIDRQLRGRCARQG
                     DPGSSQFYISLEDNLMRLFGSDRISGIMTRLGMQEGEALEHKWLNRSVETAQRRVEQQ
                     NFAIRKRTLEYDDVMNKQRSVVYDLRGDVLTAETAHNHILDVFNDLILTQSERYLSSA
                     KDAMPQELVRWVTDTFPVAVREEEIAAFRGEPEKAGELLYTRVAEAYELKCSMEDPAV
                     LPIMERSVFLSCIDQQWQSYLRAMDELRHGVNLRAYGQRDPLVEYKREAFTMFEELMT
                     NIKTDVATSVFRATTMQNFQRMMAGPGGRVQMVHNDINILAGGETPQPAAGQAGRGLT
                     AADVPQAAVAGFDAILDAMDRTGQSAPHATTGPLIAKPSVGRNDPCPCGSGKKYKKCC
                     G"
BASE COUNT         2735 a         4421 c         5238 g         3016 t
ORIGIN      
        1 ccgccgagag gcctcgcccg tcggcgaaac gatagtacgt cgttgttgcg ccgacgcggc
       61 aacgggtctg cggcagcacc ggcagttcga cgagatcccg gggaccgtgc ccacagaggc
      121 ggtacgtgat gccgtccgcc tccaacagaa tgacaagcga ttgcgttgcg cccgcccaat
      181 gtgtcgtgtc gcgttcgtaa agccgatctg ccgcactcca gagactaaaa tgaggctcga
      241 ccgagacgag cggcaccgct ggtggacgaa acgccgccat tgcgtcaatt gaaatcgcgg
      301 cgacaaggcc gcagaacaat gctctttcaa tcgctttcat ctttctgtcc cttctttttc
      361 ggtttggacg atgtatagag ggtttggggg gttgttgcaa gggtattgtt gagaccgtca
      421 agaggcttca atggggagtg taaaacggga acgcggggtg gacggccccc gcgccatgct
      481 tgcgcttgtt tgggcaaggg gatacgggta tactcaacgc atgatttcac aggatcaggg
      541 caaggcaagg gttgtcgtga ttgtcggacg ccccaatgtc ggcaagtcgg ccgtattcaa
      601 ccgcattgcg ggcaggcgta tcgcgatcgt gcatgacgag agcggggtga cccgcgaccg
      661 gttgatgcgg gaggtggagt gggacgacca gcgtttcacc ctgattgaca cgggcgggat
      721 caccctcctg accggccaga cgggaaagaa cgcgatcgag gcggggatcc gcgctcaggt
      781 cgatgcagcc atcggggatg ccgcggtggc cctgctggtc gtggatgtga aacaggggat
      841 gcatccgttg gacgaggagg tcgcgctcat cgtgcgcaag gccgggattc cctgtgtcgt
      901 ggccgtcaac aagtgcgatg agccgcagca cgaggcggga gtcgctgaat tcgcgcgttt
      961 gggttttgcg acctttccgg ttgcggcgca gcataaccgg gggatggggg aactcatgga
     1021 ggcggtgctg ccgtacctgc cggaggcggt caacccgacg gaaacccagc cgctgcgcgt
     1081 ggcggttgta gggcggccga acgcgggcaa gtcctcgtac atcaaccggt tgttgcgcag
     1141 tgaccgcgtg atcgtttcgg atgtcgcggg cacgacgcgt gactccatcg atgtgccgtt
     1201 cacgatcggc tccggcgcac aggcgcgaca ctatattctc gtggacacgg cgggcatgcg
     1261 caaccggcac cgcattgaca atgcggtgga gcgcttcagc ttattccgtg cggaagagag
     1321 tatccgtgga gccgatgtgg tggtgctgat gctggatgcc gagatcggcc ccaccgttca
     1381 ggataagcat atcgcggcga tgattcagaa gtataacaag gggtgtgtgc tgctgatgaa
     1441 caagtgggat cgcgcgatgg agaacgggat gacccagacc cggagcgaac cggtgttgcg
     1501 tcagatcatg cccttcatgg ggtatagccc ggtgttgttc atctcggcgg ttaccgggtt
     1561 caacgtgcgt aaaagcgtgg agaccatcga tgccgtggcg gctcaaacac gggtgaccct
     1621 gccgaccggc atcctcaacc gcacactggt cgaggcgacc gagcgtgtgt tggcgcctgc
     1681 ccgcaacggc aagcgcctgc gggtctacta tgccgtgcag atcggagtcg cgccggtttc
     1741 catccgtctg tttgtcaatg accccaaact cgccaccaaa ccgtacacgg atttcctgat
     1801 tcgctccctg cgttcgcgtt tcggtttgga gggggcgccc gtgcggttgt tctaccgcgc
     1861 gcggacgcgg cccgagtcgg tggaacgccc cgcgagaagt cgcagccgcg cgcgcgggaa
     1921 aaaacggaaa gcccgatgag ggggcggtag gtttgtttcg taaaatttct atacagtggt
     1981 ggcggttttg gatggcgggc gagggattgt ccgtcaagac cggtcgtgcg ctggtgcttt
     2041 gtgcattgac gggactggtc gccggtttgg cggcatccgg attctactgg ctgctggagg
     2101 cggggcggta tctgctgttg gaacggctgg ggcactactc gccgccgttg gccgctggcg
     2161 agttcccgct cttcccgccg gtggcgcacg aggcaccggt gcgctgggtt ttgctgttat
     2221 tgccggcggt cggtggtctg ctcagtgcgc tgttggtgaa ggggttcgcg ccctcggcgg
     2281 gcgggcacgg cacggatgcg gcgatctatg cgtaccaccg caatgagggg cggatcccgt
     2341 ttgcggtgat cccggtgaag gcgctcgctt cggcactggt catcgggtct ggcggctcgg
     2401 ccggttgcga gggcccggtg actcagatcg gcgcggggtg cggttccgcg gtggcggggc
     2461 ttctgaagtt ggatgcgacc gagcggcgta tcctgatggc ggcgggattg gcggcgggtg
     2521 tgggcgcgat tttccgcgcg ccgctggcgg gcgcgatctt tgcggccgaa attttttaca
     2581 gcgggctcga catcgagtac gaagtgatga tgccctccct ggtcgcctcc acgatcgcct
     2641 acaccgtttt cgcgctgttt ttcggctggc agccgctgtt tgtgatgacg ccctataatt
     2701 ttgacgcccc gctcaaactg gggccctatc tgctcttggc gttggtggtg gccttcggcg
     2761 cgaaactgta catccatgtg ttccgcagaa ccgaggcgtg gtgccgtaat ctgtcgcggg
     2821 tgccaacgtg gctcaagccg gccctcggcg gtatgctgac cggcctgatc gggttcttcc
     2881 ttccggaggt gctgggctcg gggtacggtc tgatccaaca ggcgctggcg gtcggcagtc
     2941 cgctggccga acggttcggc tctccctcgt ttacgatgtt atggctgatc tttttcggga
     3001 aaattctggc aacctcgttc acggtcggca gcggcgggag cggcggcgtg tttggtcccg
     3061 cactggttgc cggatcaacc ttgggtgcgg caacagggct gaccctgatg cggttgctgc
     3121 ctgcgttgga tctgcatccc ggcgcgttcg ctctggtggg catggccggt tttctggcat
     3181 cctgcgtgcg cgtgccgatc gcggcgatca tcatggtcag cgagattacg ggaaaccatg
     3241 aactgctgtt gccgagcatg tgggtgtgcg gcatcgcgta ttggatcggg aacgggtgga
     3301 cgctgtaccg cagtcaggtg aggacacgcg actgttcgcc cgcgcacagc cggtgaggga
     3361 cgcggtgttc gggtcccggt gcggaacgtg gatagggagg ggtggaggat gcgtattctg
     3421 gtgatcgacc gttccacatc cagagcaggc atggcgctga tggatgcggg cagagcgatt
     3481 tgttcgcggg tctgggagga tgacgccgcg cggtcgccgg gttggattgc ggaggcggtc
     3541 cgggagttgg ccgcatgcgg ggtgggactg caagaggtgg gccggtttgt gtgcgggttg
     3601 ggaccgggct cgttttccgg gatccgttcc agcctcgcgg cgctgcaggg gatggcgctg
     3661 ccgacaggtg cgcccgtctg cggtgtggcc agtgccgcgg cgctggcgct cgcgtacggg
     3721 atggacgtgc cgcgggtaac ggtggtgggc gacgcgcggc gggaacggtt gtggattgtg
     3781 acatatcggg tggaaccgca cacggggcgg gtgatgctgg cggacgggac gcgtccgacc
     3841 cacacggctg cggatttcac gctggtgccg gcgtcggaat tggcttcggc gatacccggt
     3901 gacgcgtttg tggtaagttc ggattgggcg cgtctcgcgc cgcgtctcac ggccgcatgt
     3961 ccctccccca gattacttcg tgagaccgtc tgtcccgatg ccgcggatct cggccggctc
     4021 gcctgcgccg atctggacgc atgcgtcacc gagccgttgc cgatctatct gcatcccgca
     4081 gtcgtggtga gataatgtga tccgcaggtt acattgaacc taaaaaaggg cagttgaact
     4141 ttcaacgctc ttcggtttca ggtttcatgc atggctcgcc ctacctgttc acagcgtcag
     4201 cggtacacgt tcacgtacac gtccgcgaaa cacgaacagg gtgccgattc cgacagcgat
     4261 cccgatttcg acgccgttgc tctgcaagtc cggcaaatgg attttgcgtc agcaagcggt
     4321 gttccgcacg ccgccgcttt ctgatcagga gtgcggtggc agggcaatgc aatgcgcgac
     4381 gcgacggcat ggctcggcgg cggtttacgg ggaacccgcc ctgccttttt caacttcgtt
     4441 ctccggcaca ctcacgcgtc ggagcgcgtg gtcccccaac cgttcgcgca cctgatcgag
     4501 tgtacggcag agccgttcgc gtttttcgcg cgcgtccgga acggaatcga agagcgcgag
     4561 ttgttcggcg ccgcactcgt tacagaggcc gctgaccccg aatccgatca ggcggacggg
     4621 ttgcgtgagc ggttgagcct gaaaaaggcg gagcgcctgt tcgcggaagg cgaagtcatc
     4681 gcataccggg ctcgggaacg gggcctgacg tgtgagggta cggaagtccg cccaacggag
     4741 tttcagtttg ccgagggtgg cgtagcggtt ttggaggcgg atccgccgcc ccacatcatc
     4801 ggccagtgtt ttcagaacct cgcaaacaac ggaacggtcg cggcagtcct ctggaaaggt
     4861 gtgttcgcgg gagatgcttt tttcctcgaa atcggtttgc acggggcgcg cgtcctcccc
     4921 gtgggccagg gccatcaggt gcgttgccgc gtgttcgccc acaaggctcg cgagaaacgc
     4981 cgggtctgcc tgccggatgt cggcgatacg gcgcaagcca cccttttcga gcgtttgggc
     5041 agtgacgcgt ccgacccccc acagcgcacg gacgggtagg ggggcgagga aggcgagaat
     5101 cgcatcggtc gtctcgggaa tgacggcgag gccgtccggc ttggcctgtt cactggccag
     5161 tttcgcgagg aacttgttgt gcgcgatccc gacggacgcg gtgagtccca cttcgttgcg
     5221 gatcgcctca cggatggccc gcgcgatctt ctccccgctg ccgaagaggg tgcggccgcc
     5281 ggtgacgtcg agaaaggcct cgtcaatcga caacggttcg atcagcgggg tgaagcgctc
     5341 gaagatggcg aacacctgct gtgaggcctc ctcgtaccgg cccatatcgc aggggacaaa
     5401 gatcgcatgc gggcagcgcc ggtaggcctc ccgcgaaggc atggccgaac gcacaccgaa
     5461 cacgcgcgcc tcatacgaac aggtggacac caccccgcgg cggtccggag gcgcgccgac
     5521 aatcaccggc ttgccgcgcc attcggggtg gtcacgctgt tcgaccgccg cgaaaaaggc
     5581 gtccatgtca acgtgtagga tgcaccgttg catagccgac tccggtcagg gcacaagaat
     5641 acggccggga accgcgccga tccgcctcac ggtctccggc aacaacaggg ttcagtcaag
     5701 gcgcggcggg cggacggggt ggcgggttct gcggcgcact ggcagcggac tgtccggatg
     5761 cagcgccctt cgccggtttg gctgccagca gacatcccaa cgtgcggccg atcagacgcg
     5821 ggttttgttc gaccaccgag cacgccgcac actcttggat caccttctgc acgacttcca
     5881 tgcccagttc tttgtgcgcg ttttcgcggc cgcggtactg tagggtcacc ttgaccttgt
     5941 ggccatcgtt gaggaactct ttgatttgac gcagcttgtg ggcgaggtca ttcgaatcca
     6001 cgctggcgtg gaatttcacc tctttgatct gcacggtctt ctggtttttg cgcgccatct
     6061 tccgcttaat gctttcctcg tagcggaact tgccgtaatc catgattttg cagaccggcg
     6121 gttcggcatt cggggacacc tcgaccaagt cgagtccctg ctggtcagcc agacgttgcg
     6181 catcccgcgt cgccatgatg ccgagcatct cgccgttggc gttgatcacc cgcacgttgg
     6241 ggacccggat tttgaagttc actcgggtaa acgaagcgat aactcacctc cgatgttgag
     6301 ccgacttacg gctcgtttct gttttgtttc cgctccggat gaaccggagc ccgcatcctg
     6361 tctttatccg cctccgggta cgcgaaggtc acgcgttcat cgcgtcagcg agcacgctcc
     6421 ggaaggcctc gatcgtcatc gcgccttgat ctccggtctc acggctgcgc accgaaacca
     6481 cttgggcttc ggcatcgcgt ttgccgacca ccagcataaa aggaactttt tcgagttggg
     6541 cgcggcggat cttcgcgccc agcttctcgt tgtcggtatc gaccgtcacg cgcacatccg
     6601 cttcgcgcag agtcgtttcc aacgagcggg cgtacgcgat ctgatccgcg gtgatgggta
     6661 gcacgcgcac ctgctccggc gccagccaca cgggaaacgc gcccgcatag tgttcgatca
     6721 gaatgccgaa aaagcgttcc aggctgccga gcagggcgcg gtgcaccata tagggctggt
     6781 gctccttgcc gtcctcgccg atgtatgtca gcgcgaaccg ttccggcagg ttgaagtcga
     6841 actggatggt gccgagttgc cacggccgtc cgagcgcgtc tttgatcttg aggtcgatct
     6901 tcgggccgta gaacgcgccg ccgcccgcat ccacctgata ttcgatggcc tcggcccgga
     6961 gcgcgttttc caaggattgc gtggcctgtt cccaccgttc aggcaacccg accgccttct
     7021 cgggccgggt ggagaggaag gccgtgatct cccggaaccc gaaacggcgc agaacgcgca
     7081 gcgcaaaggt cacggtggac cggacctcat cctcgatctg ctccggcgtg cagaaaatgt
     7141 gcgcatcgtc ctgcgtgaac ccgcggacgc gcaacaaccc gtggcgcacg ccctctttct
     7201 catagcggta cacggtgccg agttcggccc atcgcaacgg caggtcacgg taggaatagc
     7261 gccgggtttt gtacacctgg atatggaacg ggcagttcat gggcttgacg aaatacgatt
     7321 cgaccgaagc ccgcgagcct tcgccctccg acagattcat gctcgggaac atgctgtcac
     7381 ggtagaaccc cagatgcccc gaggtttccc acagtttggc gcgccccaca tgcggggtga
     7441 acaccatctc atatccggcg cggaagtgtt cgcgccgcca atagtcctcg atcgccaccc
     7501 ggatgcgcgc gcctttggga tgccagtgcg ccaatcccgg cccgacctcc tcgctgatac
     7561 taaacaggtc aagctctttg ccgagtttgc ggtggtcgcg ttttttggcc tcctccatcc
     7621 gggcgagcac cgcgtccagg gcctcgcgcg acacatccgc catgccgtac agacgctgca
     7681 gcatggcgtt tttctcatcg ccgcggtagt aggatcctgc gacggacgtc aacttgaccg
     7741 cgccgatttc cgaggtgcgg ttcacatgcg gtccgcggca caaatccacg aacgcgccgc
     7801 agcggtaaag ggtgatcgcc tcgccctcgg ggatgtccgc gaggcgttcc aacttgtaag
     7861 gttggtccgc caagatccgc gccgcttcgc cgcatgtcgt ttccaagcgc tcaaacggca
     7921 aatcggcctg gataagggcc tcgatctccg cctcgatacg cgggaaatcc tccggcgaga
     7981 gccggtgctc cagatcgaaa tcgtaataga acccctcttc cgtcgagggg ccaatatcca
     8041 acttcacgtt gtcaaacaga ttgcataccg catgcgccat cacatgggcc gcactatggc
     8101 gcatcacatc gatcgcttcc actccaacct ccatgccgga tgcccgtaac agccaccgga
     8161 accagtcata tccgaacagg atgaagtgtc ccgtatccgt ccgcggttgt caagtgggcg
     8221 cttcggtgaa cgtagatgaa aaacaaggtt ggttttatga aggttcatgt ttttcacggt
     8281 gcggaccccg agacttggaa ccccaagcca gaaacccgac aaagcgtcac ggttcggcgt
     8341 tcaccaaagt tgaatcgctc ccgggtggga aatcggttga ctccgtcagg actccgcacc
     8401 cataatgaga cgttgcggtg agaattctgg tccggacagg gtggagtcgg cgggaggcag
     8461 tgaaagggag gtcggaaatg cgaggtcgtc tcgggttggc gtgggtgttg gcgggaagtc
     8521 tggctttggc ggagggggtg gtggagagcg cgaaaaccct gccgttggcg cacgagacgg
     8581 atgtggtgat cgtgggcggc tcttccggtg cggtggcggc cgcctgccgc gcggctgccg
     8641 aaggggtgcg ggtttttgtg gtcgcgcctc gcccgtatct gggcgaggat atggcgggga
     8701 agctgtgtct gcgcacgggg gcctcggaag atccgcgctg cgttttgcgg cgccggatgt
     8761 tcacgacgac gcaacttccc ccgtcgctgc ccttctccta ccggagtgac cgtgccgccg
     8821 cgtcgaatca tccggacccg catcaccgcg tgttgtcgga cggagtgtgg caggatgccg
     8881 tcacggagag tgcccagttc ggcggtgatg tgacgctgac gcttgatctc ggggcgctga
     8941 ccgaccttgg ttcgttggtc gcctgcgtgt ttcggcgcga cggggccaac gggttccgta
     9001 ccggcgcgct acacgtttgg gggagcacgg atgcggaaac gtgggcggat ctgggtaagg
     9061 ggatcgatca ggaaaccgag gccggatccg aacgcgccag attcagagtc ccgctttccg
     9121 gtcggtatcg gtatgtgaag gttctggcct tggtggcgcc cggtgtgaag cggcagttgc
     9181 ttggcgaact gctggtgtac cccgttgccg atccgtcggc tggcgcggga ctgtcggacc
     9241 gtacgacgcc gcttcaggtg aagaaagttt tggatgaggc gctgttgcag gcgggagtcc
     9301 cgtttctcac cggcgcgctg gcgaccgaga cgctgacgga cgaaaccggt gcgctggcag
     9361 gggttgtgat cgccaaccgc agcgggcgtc aggcgatccg cgccaaagtg gtgatcgacg
     9421 cgacggaacg cggcatcctt gcccgtgccg cgggcggtca ggcggaaccg ttcccggccg
     9481 gacactacac gttcacccgt cgcattgtgg cgggcgaggc accgcgcgcg gagggggtgc
     9541 gggtgcggga gttgttcggg gtctacccgg tgcgtgtcac gggcatccgt ccaccggagg
     9601 ggcatcccgg cacgatcacg ggacgtctgt tcgaatgcga gatcgacctg ccgatgcggg
     9661 acggttcagc acgcgccttt gccgaggcgg agcaacgcgc gcgcgatctg acctttgttc
     9721 ccacgcagtt ggaatcagcg gatacgcttt ctctgcttcc gcccgacttc attcggggca
     9781 aggccgctgt acgggcgccc tgggccgggg ttgacgcgct tgatctggat gcgtttcaac
     9841 cggctggcac gtcgtttctg tttgttctgg ggccgatggc ggatgtcgcg cggccaacgg
     9901 tcgagcactt gatgcggccc ggtaatctga tggccttggg tgagcggatc ggcgcggcgg
     9961 ccgcacggct ggcgaaacag cgcccggcac cgcagggcgt gcgtgcaggg gggagtccgg
    10021 atgcgcaaag cggcggagtc acggtcggcg aacatacccg caatctggcg gcctatctgc
    10081 ggaacgcctc cggcacagtt cgggtcgagc cgcgtgaatt gccggttctg gctgagtgcg
    10141 atgtgctggt cgcgggggcg ggcaccggcg gggcgcccgc cggaatcgcg gccgcgcgtc
    10201 atggtgcgcg gaccctggtc tgcgaatata ttcatcagat gggcggcgta cagaccgacg
    10261 ggcttatcgg ccgctactat ttcgggaacc gcgtgggctt cacgctggat attgaccggg
    10321 gcgtgaaaga gaccggggcg gtcttttccc agtgcaagtc cgagtggtac cgcagccaga
    10381 accggaaggg cggggccgag atctggtatg gaacgatggt cagcggggtg gtggtggaga
    10441 acaaccggct caccggcgtg gtggtggtca tgcccgacgg cacacgcggc gtggtgcgtt
    10501 gccgcgttgc gatcgacagc accggtaatg cggaactcgc ggccatggcg ggcgaaaaga
    10561 ccgagttcat caccgccgct gaagtggcgt tgcagggtgt gggccagacc ccgcgcagga
    10621 tgggcgcgag ttacaccaac accgatatcg gctttctgga tgatacggac gcgtccgacc
    10681 tctgcttctt cgcgttgcgt tcccggtaca gtatgcggga ggggatctgg gatcaggcgc
    10741 agatcatcaa cagccgcgaa cgccgccgga tgatcggtgc cttttacatg tcgccgctgg
    10801 atgtggtcaa cggacggacc tatccggatg tggtggtcca gccctacagc aacttcgact
    10861 cgcacggcca tacgacccac gagcagtttt tcattgagga tcccggccat cacggaatga
    10921 aggtcaactt gccgttccgg tgtctgctgc cgaaaacggt ggacgggctg ctggtgaccg
    10981 gactcgggat cagcgcacac cgcgacgcca tgccgatttt acgcatgcag ccggatgtgc
    11041 agaatcaggg ctatgcggcg ggcacggcag cggccatggc gatcaaggcg ggtgtgccgg
    11101 tgcgtcgggt ggacatccgt gcgctccagc gtcatctgat cgaaatgaag atcctgccgg
    11161 agacggtgct ggacatggag gattcctttc cgcttccgga cgcctgcttc accgaagcgg
    11221 tggccgcgat ccccaatggc tatcagggac tgtcggtggt gctgacggac cccgcgcgca
    11281 gcgtgccgct cctcaaagcg gcctacaccg cggcccgcgt acccgaggcc cgtttggctt
    11341 acgcgcatgt gcttggcatg atggggcatc ccgatggtga gacggaactg attgcgcggg
    11401 ttcagggcat ggcctgggac aaggggtggc attttcgcgg gatggggcag ttcaaccgca
    11461 gtgtgagctg gctggacagt tacctgatcg cgttgggacg ctgccgctcc gcacgcgcgg
    11521 tgcccgcgat tttggacaag gcgaaggccc tgaccgcggc cgacgccttc tcgcatttcc
    11581 gtgcggtggc cttggcgctg gaggcgatcg gcgatccggc gggcgccccg gttctgggac
    11641 ggctgctcgc tacggagggc atcggtggtt acgccatccg tatgggaccc gaactcccgg
    11701 tgattcccaa ctacgcgaat cacgaaggcg accgggagcg ttccgcatgt ctgcgtgaat
    11761 tggcgttggc gcgggcgctt ttccggctcg gcgatttcga ggggcgtggc gtgcgcacgt
    11821 tgcgggccta tgccgaggat ccgcgcggcg cgtatgcgac gcatgctgcg ctggtgctgg
    11881 gcaggtgatg cggagcagcg ggttacatgt tacggcttat tcgcgtgcgt gaacgggtaa
    11941 ggttaatctg gcgtattcgg tgtgatgtga tacacttttt tctttcattc ggatggatcg
    12001 gaaatatccg caggaacagg gaaacgcatg aatctgatca gaacactttt tggaaccaaa
    12061 aacgaacgcg atataaaacg cctccagccg atggtcactc ggatcaacga gttggaaaaa
    12121 gagtatcagg cactgtcgga gcagcagctt caggccaaaa cacaggaatt caaggagcgc
    12181 gtggctgcgg gagagtcgct ggatgacttg ctgtgcgagg cgttcgccgt ggtgaaaaac
    12241 gcgtgccgtc ggctttgcgg caccacccgc gaggtgtgcg ggcacatgct ggcttgggat
    12301 atggttccgt ttgatgtcca gctcatcggc gggatcgtgc tgcatcaggg caagatcgcg
    12361 gaaatgcaga ccggtgaggg caagaccttg gtcgccaccc tgccgctgta cctcaacgcg
    12421 ctctccggga acaatgtccg gctggtgacg gtcaacgact atctcgctcg gcgcgacgcc
    12481 caatggatgg ggcatgtcta tgagtatctg gggttgacgg tgggatgcat ccagaaccac
    12541 atggggccgg ctgagcgtcg ggaacaatac gcatgcgaca tcacctacgg caccaacagc
    12601 gagttcgggt tcgactacct tcgggacaac ggcatggcgg tggagccgtc gcaggtggtg
    12661 cagcgcggcc accatttcgc gatcatcgac gaggtggaca gcattctgat tgacgaggcg
    12721 cgcacgccgc tgatcatctc cggtccggtc gcgcatgtgt ccacggcgca gtacacggaa
    12781 ctcaggccgc gtgtggagcg gctggtccgc gaccagatgg agatctgtaa ccgcctgatc
    12841 agcgaggtcc gcgaggccat tgaagctgcg gacagcgaaa cggcgatgtt caagctgtat
    12901 caggtgtacc acggcatgcc caagcacaag cagttcctgc atctgttgga ggaccccgcg
    12961 acgcgcaagc tgcatgagca ggtcgagagc atgatgctct ccgaaatgcg caaagaactg
    13021 gcccgcgacc tgcgcgagca actgcttttc acgatcgatg agcgtacgcg ggaggtcgcg
    13081 ctgaccgacc ggggcagtga gaagctctcg ccgaccgatc cggaaatgtt cgtcatgccc
    13141 gatctggtct ccgccatgtc ggcgttggat ggcgacaccg cactgggcgc ggaggagcgc
    13201 gccaaacggc ggcagcagat tcaatccgat ttcatggtgc gcagcgagcg cgtccacaat
    13261 gtggaccaat tgctgcgggc ctactgcctc tacgagcggg acgtcgatta tgtggtgcag
    13321 gataaccacg tctatattgt ggacgagttc accggccgcg tgctgcccgg aaggcgttgg
    13381 agcgatgggc tgcatcaggc ggtggaggcc aaagagggcg ttgaaatcga gcgcgagacg
    13441 cagaccctcg ccacgatcac catccagaac tatttccggc tgtacaaaaa gttggcgggc
    13501 atgaccggta cggcggagac cgaggcgtcg gagttccacg acatttataa gttggatgtg
    13561 gtggtgatcc cgaccaaccg gccgatacgg cgcgtggacg gtaacgatca gatctacaaa
    13621 tcgcagcgcg aaaaatacaa ggccattctc gacgaggtgg agaagcgcca ccggaacggg
    13681 cagccggtgc tgttgggcac ggtgaccgtg gacacctccg agatcctcag ccgcatgctg
    13741 cgcatggcca agatccccca caacgtgctg aatgccaaga atcacgcgcg cgaggccgag
    13801 atcgtgtcgc tggccggaca gcccggcgcg gtcaccatcg cgaccaacat ggcgggccgc
    13861 ggtacggaca tcaagctggg tgaaggcgtg gtgtgggtgc cggagacgac gatccggtcg
    13921 cagatcacgc tggaaaaccg ttatgacaac gggaacaaga ccttgcgcaa gctgctggag
    13981 gagaagccgt gcggactgca tgtgatcgga tccgaacgtc acgagtcgcg ccgtatcgac
    14041 cggcagttgc gtggccggtg tgcgcgtcag ggtgacccgg gctcctccca gttttacatt
    14101 tcgctggagg acaacttgat gcgcctgttc gggtccgacc gcatttcggg tatcatgacc
    14161 cgcctcggca tgcaggaggg cgaggcgctg gagcacaaat ggcttaaccg ttccgtggaa
    14221 accgcgcagc gccgcgtgga gcagcagaac ttcgccatcc gcaagcgcac gctcgaatac
    14281 gatgatgtga tgaacaagca gcgttcggtc gtgtacgacc tgcgcgggga cgtgctgacc
    14341 gctgaaacgg cgcacaacca tattttggat gtgttcaacg acctgatcct gacgcagagc
    14401 gagaggtacc tttcctcggc caaagacgcg atgccgcagg aactggtgag gtgggtgacg
    14461 gacaccttcc cggtcgcggt gcgggaggaa gagatcgcgg cgttccgggg cgagcccgaa
    14521 aaagccggag aattgctgta cacgcgggtc gcggaggcgt atgaactgaa atgttccatg
    14581 gaggatcccg ctgtgctgcc gatcatggaa cgctcggttt ttctgagttg tatcgaccag
    14641 cagtggcaga gttacctgcg cgccatggac gagttgcggc atggcgtcaa cctgcgcgcg
    14701 tacggtcagc gcgatccgct ggtggagtat aagcgcgaag cgttcacgat gtttgaggaa
    14761 ctgatgacca acatcaaaac cgatgtggcc acctcggtgt tccgcgcgac gacgatgcag
    14821 aatttccagc gcatgatggc gggtccgggc ggccgggtcc agatggtgca caacgatatc
    14881 aacatactgg cgggcggcga gaccccgcag cctgcggccg gacaggcagg aaggggtctg
    14941 acggcggcgg atgtgccgca ggccgctgtg gccggattcg acgcgatcct ggacgccatg
    15001 gatcgcacgg ggcagagtgc gccgcatgcg acgacgggtc ccctgatcgc gaagccctcg
    15061 gtggggcgta acgatccctg tccgtgcggc agtggcaaaa aatataagaa atgctgcggc
    15121 taggggttgt aatctgactg ttgcgcagtc aggttacagg ttagcccgcc gcgcgccgga
    15181 gccgcgagaa aactctggga tacattttta tgacaggatg acaggatttt caggattaaa
    15241 aaaatcatgc taatcctgtc atcctgtcaa tttaccctaa aaagagcagt tgaaccttca
    15301 acatctttgg tttcaaggtt tcaaggtttc aaggtttctg gtttcaaggt ttcaaggttt
    15361 catcggggtc gggatcgcta tcgggatcgg gatcgattgt ttcaccacag
//
LOCUS       DAOSRB010000049        15305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_3978_length_15305_cov_7.482754, whole genome shotgun sequence.
ACCESSION   DAOSRB010000049 DAOSRB010000000
VERSION     DAOSRB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15305
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3978_length_15305_cov_7.482754"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..436)
                     /locus_tag="PLZ74_04250"
     CDS             complement(266..436)
                     /locus_tag="PLZ74_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR97566.1"
                     /translation="MAYQITDKCVACGLCKDACPVEAISEGTPIYTIDADKCIDCGAC
                     SGECPNEAIIAG"
     gene            complement(563..2164)
                     /locus_tag="PLZ74_04255"
     CDS             complement(563..2164)
                     /locus_tag="PLZ74_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOR97567.1"
                     /translation="MLRVFLSGAALLLTCLSAFGAAARFTAPRNYPNIGLKLPGLDSA
                     RADPVPMPQAQAFLSVRGTVLSREDRFQPFELWYGNQCCAQWRDAAGNRLVLGRATHR
                     LPVFEEETVPRERFEQAMQDGSGSEGFRDRAALNEWVATFADTPVYDPEPLRLNAFSL
                     DEVLFYPCGEADKLVYAILPRRQGSAERPDWFCVTFHAPGASDPDALREHFEEAFLSQ
                     ISQPSRTEVDQGPQAEELDVRSAKGARPPDLPDHPVRREARKSVENYGDWWFAETEGT
                     IILSDADTDLGKRLIQTLQETLPPLWKAFAQLVPPLTRDRDVALLRLFQRRADYARYT
                     GPAYAWTGGMWMPGRRELVLPLEADPQVVLRTVRHESFHQYLSFAYGMVPAAPWFNEG
                     HACLFENARMDAEGRVILDEDTDRVFVLLDHLDTVLTLLPHLLHASYEEFYAGFSAGR
                     RLKYAMAWGLVYYLQKGAPTERNTPFKQILPTYADALALELDGAKATEAAFADVDMAV
                     FLDTFREFWLKRRGTAMRYDPLQQR"
     gene            3267..3824
                     /locus_tag="PLZ74_04260"
     CDS             3267..3824
                     /locus_tag="PLZ74_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOR97568.1"
                     /translation="MSKKIYISANAYLLDSFRLARRILDSGWLPDDLIVLWRGGAPVG
                     MAIHEFLSYHGLRPRHRTLKCYSYTGIRTHREEVAFENADAVFSSVTPGSRVLVVDDV
                     FDTGNTARAVLDRLAPSGAETRLATVYWKPNRNQTSGKPDFYLHETDEWIVFPHELEG
                     LTPAEVHRKDPALHALLKSPASQPR"
     gene            complement(3812..4996)
                     /locus_tag="PLZ74_04265"
     CDS             complement(3812..4996)
                     /locus_tag="PLZ74_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOR97569.1"
                     /translation="MAKMFTAAPRVVVILETCVQPDREKLNGILRYVRFYGPWHLHII
                     QNRVGEQRLAHFGAWHADGVIVGQSMLDMERTLARAKMPIVLIDPLPRHLQPDSPFRH
                     CCRIQNDAVSVGRAAAEFFLERGFERFAYVGEVMGRLWSKGRGEGFAGRLREVGRVCQ
                     VYAPPSRAEIRDWAVEQLRLAEWLASLPKPVALFAAMDIRARQVLDTCLITGIKVPDE
                     VAILGVDNDEQLCLGSFPTLSSIECDMENCGFMAARLLNEQMRGRHRKRVELTYGVKR
                     IEERHSTRQVPHMEDRLVARALEFIRLNACEGIGVRDVVAYLKVSRRLAELRFRTTCG
                     HSILDAIRAVRLDRVRRLVAETDLPVSEITSRSGYQTDDHLRRLFKREFGLTMREYRQ
                     RG"
     gene            5180..10075
                     /locus_tag="PLZ74_04270"
     CDS             5180..10075
                     /locus_tag="PLZ74_04270"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOR97570.1"
                     /translation="MTRYRQSVFLFLLPFCVVAANTPTPDLVLARKGRPADRAIVCPV
                     QASPSQRYAAEELQTFVEQMTGVRLPITDDRAPLPAKAILLGDTRHTAAVLGATPALD
                     GLGTDGFLLKTVPPHLVIAGSPVRGTLYGVYELLERYGGCRWYTADYSVIPKRDTFAI
                     PPLNDRQTPAFAIREDRWTDMIPGDVAARNRCNGHDMLLEERHGGKVRFGAGLVTHTF
                     YSLMPPREFFAEHPEYYSLINGKRRAENAQLCLTNPDVLKITIARLIERIRKDPNAQI
                     YSVSQEDNRAYCRCAACEAINQREGTPAGSLIAFLNQVAEAVEKVYPDVMIDTLAYQY
                     TRTPPKTLKLRKNILIRLCTIEFDFSLPVNQSTKASNKRFIDEIRGWQALTDNLYIWD
                     YVTNFGSYVGVYPNFGALQGNLRFYRDHGAVAIKQQGARPGKHADFAELKAWVIAKLL
                     WNPDQPLEPLVTDFLEGYYGAAAPHVRAYLDATTRLVADPDIHIGCFNRPETYPWLTD
                     DLLAKFADTFRQAEQAVRNDPPRLHHVRMLGLGVLYTQLRRIPLPAAPQIIWAADGAR
                     LNNIPSATEALAREVVARLDEEPGILLLQNSLTHHVIQAQYRALAGRVPHHTFSNDAI
                     TASLTELENAKLGWLAKTGDPAFNYLSPNRDWGVQIQPDQQERTAAELFGMVYRKAKT
                     SQDAPRFALSRLGAISSERHLKTEGSRLLFSARFRNTRQTLQQETEPMIRAALNLGQH
                     GRFCWRTAPDAPWRTQSELREPVVALSAAERGTGQIEIASPVTRRMLRLTFDPARAVS
                     VTLRLHQAEAESVRVQITEKPETLAPGAERVCPLTLEIADPPPDLPGSLATAFVTPPV
                     GQPPHIVWQGQPAARIVVPVRATPVERSAARELTLHLEAICGAAFETVTEDEPKTGPR
                     LLVGNTDAARRLIPGFDPASLAYDGILLKTAGDDLILAGHPQRGPIYAVHTFLEDSVG
                     VRWWTSDETFLPQHTSLPLPTLDLRYEPKLKFRETYYLDSFNARFKVRMKGNFSSRTR
                     FMLDPLEMIPEAYGGNHRLIHFAGRNSAYHSFYELLPPAKYFKAHPEWYSEINGKRTH
                     TRAQLCLTNEEMRRELTKNALALLRTDPGADFIQISQNDHAGRCTCARCLAVEKEEGG
                     VGTASGPLLRFVNQVAEAIEKEFPNVWIDTFAYQYTRKAPTKTRPRRNVLVRLCDIEC
                     SFLHPIEGTPQNAKFLTDLVEWSRIAPGNLFLWDYVTSFSSYILPHPNLRVLAPNVRT
                     FVKHGAIGLFEQGDALCRAGDFVRLRQWVLAHLLWNPDLDADALTDTFLAGYYGPRTG
                     AALRRYLDFLHDRAARADVYLGCYQNNVTHWLDAEGILEALRLMDAALAEAKAEEQAD
                     PVGRKGLADKIRREKLPMDHVVILNRPLLQRHAKATGKPFPVTADPLAAVEAWIAACE
                     RFGVKAHRETTTADTFIDYKKSLLERARMASQKVSPPPCCATLPPASWHDVQEFEFDQ
                     RPAGQVAFVEDKAASNKMAAQMPGNHKIWAVQFPLTSFIPLSGTASGTPRCTLHVYAR
                     ADATATEGNGLALGVYDFTARKTVAHQWIPLSELTGPGYKMFKFGPLPITEGCKFWAN
                     PPERTGEVQHVYVDRVVVVVE"
     gene            10128..11540
                     /locus_tag="PLZ74_04275"
     CDS             10128..11540
                     /locus_tag="PLZ74_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97571.1"
                     /translation="MKKMVFIVTAGLSACLLHAASWVPTQPSPDEAAIACINTSVTNG
                     TDVDITATLASNTAEVVIGTALSDRHTFLRAVDLSGRTGTGLGSADNSAFHAAGVDDA
                     ATGAAILLKRLSGDVHGAGVTHAFGLLAWSDGALGNTPATLQIDHLTGTVSANVHPDG
                     TAAALYARTALHLNLMPGALLFGGSYGWDLGDQTLTNAQAELVGLLAKVREGAATASE
                     SNTLKTAASGYAILGGEEDDFLRFGGLATLFGQVDLGDGMDVVSLCDLPSNTVTRLPA
                     IANAELLLITNSPIFSASLAGAPKETVIAGDSFCTFVDELTTLDSLSGTGTLASEGTI
                     WVTNLLSVSGFLAAPTGSALIIEGNLGLADGLACSLRACGTADGWTNDRITVTGTVTV
                     DGGGTVDLGCTPSDPIPEKERRILMTVAGGVINSANLASWTLTGTGWDEDPSLIRRIG
                     TDGVTIYASVLKGGTLLLMR"
     gene            11564..13867
                     /locus_tag="PLZ74_04280"
     CDS             11564..13867
                     /locus_tag="PLZ74_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOR97572.1"
                     /translation="MKNLSKPLAIFSMTACLAGTVHAQQILTTVQNITAAPAPAITWA
                     TQPDGVYSLLASDTEGGPFDTVLIASTQTVENSITYVDWRQATEGTRRYYRVVENTPG
                     YATYMIVDLSGGPDATVYPVSYTNAVPDLLTDSAYKTNKMVFRLIPGGSNITGFPEDV
                     PRGQYTPYIPLGTMTLTRPHYMGVFEVTQDQYALVMNTNPSPSNRLGATRPVQDVTYN
                     DIRGSAAQGGGGWPSNSEVYTNSFMGRLRRKTGYEEFDLPTAGQFEFATRAGTTALFY
                     NGFNDPTNTTDFSGITNIARCCGNQLSTWGGYTYAASVGSYLPNAWGLYDMTGNLSEH
                     CLDWGGRNSGTDPVGPQSGTSRTVRGGAHSTSALSTLYAGARDSMNPGKVTQSGVFGF
                     RTALRLPDTPSEEMRSSVSAGTILSVTGTPRPASRITWQTVPGKTYIVLCATNETGPF
                     ETQIGDTLTASGNTLSVTDTGYTPDGPQRHYRVEELPGGYARYRIVDISGGTNAASWP
                     VTHTNVLPDINSALYRTTRIAFRLIPPGSFTMGAPLSTVGATTKTTPHKVTLTRPFYM
                     AVFETTEAQHSLIYGDGSGTSQYPVGNVTYDMLRGSTSDWPLSSNVAPDSIIGMLRAK
                     TGGALDFDLPTEAQWEYACRAGTTSAFNNGDDLKTETDFGTLKATLGLNNRHAGGAYQ
                     PNSWGLYDMHGNLLEWVLDRWQEDLGTADVVDPNGPSTGTKFSTRGSAYMAKDSFKYA
                     SFSRENGGMNRYGSTGLVSYRLACRLD"
     gene            13895..>15305
                     /locus_tag="PLZ74_04285"
     CDS             13895..>15305
                     /locus_tag="PLZ74_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase"
                     /protein_id="HOR97573.1"
                     /translation="MNAHMKILTVSLTLALLWGFSGCRPPETARTPRDQALDFIRNET
                     QFHLGYLPTEQSHPKTRGLSQALQADTAKGIRMLLSVDEDIPPVARRVAASPEFAELR
                     TAIKTALDANKKVYFSGCGATGRLSILLDAANRRFWRETYERHPDLKAVCGDMAGRTH
                     AVMTGGDYALVRSVEMFEDYITFGYHQMEQAGAAEGDVIVAISEGGETSSVIGTILRG
                     LDVKAKVFFLFNNPADLLADKLERCRRVIRNDAVTKLVLCTGPMGVAGSTRMQATTIE
                     MLVAGMAYEAGLKEHLQTKLNSTQIASLGLDARTPEKTLEQFDTLLGQIRSERNVTAL
                     ARLADYEESLYTRGGRITYFADGYLLDIFTDTTERAPTFKIPPFRRCDDTLSPAPWAF
                     VKDPLRTTPEAWLRVLEHEPRCLDWSPETYIRLGASPDIRKNPPRIDRASLHTFLIGN
                     EPDASRTEIRPNAAMAVLIG"
BASE COUNT         3033 a         5242 c         4388 g         2642 t
ORIGIN      
        1 gtccaagacc aatagaggtt cccgctttcg tccgtggcga tgccttggag atgcccggga
       61 tagtcgcccg gacaagcgac atccgcccaa cccggaccga tgatggcgag cagtccggca
      121 accagtacga caacctgttt cacatttacc tcccgtcgtt ttcaagacac gtttttgccg
      181 aaggccgtac ccgtcggcag gcgttaaagg aaaaggcggc aggttaaccc cgccgccttg
      241 ttcatccgga taaaaccgcc gggggttaac ccgcgatgat cgcctcgttc gggcactcac
      301 ccgagcaggc gccgcaatca atgcacttgt cggcgtcgat cgtgtagatc ggggtgcctt
      361 cgctgatcgc ctccaccggg catgcgtctt tgcagagacc gcacgccaca cacttgtccg
      421 tgatctgata agccatgatg tcctccttgg atcgccgtgc cggactccac gctgaagcgg
      481 gccaccgtca cgtcaaaacc gttgctgaat cggaatgggg aacataatag cgaatggccg
      541 gtccgttgcg caaaccaaaa cctcagcgct gttgcagcgg gtcataccgc atggccgtgc
      601 cgcggcgttt cagccagaac tcgcggaagg tgtcgaggaa aacggccata tccacatcgg
      661 cgaaggccgc ctcggtggct ttcgcgccat ccagttcgag cgccagcgcg tcggcatacg
      721 tcggcaggat ctgcttgaag ggcgtattgc gctcggtcgg cgcacccttc tgaaggtagt
      781 agaccagtcc ccacgccatc gcgtacttca accggcggcc agcgctgaaa ccggcgtaga
      841 actcttcgta ggaggcgtgc agcaggtgtg gcaaaagggt gaggaccgta tcgagatggt
      901 ccagcagcac gaagacacgg tccgtgtcct cgtcgaggat cacgcgcccc tccgcgtcca
      961 tgcgggcatt ctcaaagaga caggcgtgac cctcattgaa ccaaggcgcg gccggcacca
     1021 tgccataggc gaaggagagg tactggtgaa aggactcgtg ccgcaccgtg cgcagaacca
     1081 cctggggatc ggcctccagc ggcagcacca gctcacgccg tccgggcatc cacatgccgc
     1141 cggtccacgc gtatgccgga ccggtgtaac gcgcgtagtc ggcacgccgc tggaaaaggc
     1201 gcagcagggc gacgtcgcgg tcacgcgtga ggggcggcac cagttgcgcg aacgccttcc
     1261 acagcggggg gagcgtctcc tgaagcgtct ggatcagacg tttaccgaga tcggtgtccg
     1321 cgtcggacag gatgatggtg ccctcggttt ccgcgaacca ccagtcgccg taattctcca
     1381 cacttttacg cgcctcgcgg cgcacgggat ggtcgggcag atcagggggc cgcgcacctt
     1441 tggcggagcg gacatccagt tcttcagcct gcggcccctg atcgacttcc gtacgcgagg
     1501 gctggctgat ttgactgagg aacgcctcct cgaagtgctc gcgcagcgcg tccgggtctg
     1561 acgcgccggg cgcgtggaac gtgacacaga accagtcggg ccgctcggca ctgccctgac
     1621 ggcggggaag gatggcgtag acgagtttgt ccgcttctcc acagggataa aacaggacct
     1681 cgtccaacga gaaggcgttc aaccgcaagg gctcgggatc ataaaccgga gtatccgcaa
     1741 aggtcgcgac ccattcgttg agcgccgcgc ggtcgcggaa cccttcgctg ccggacccgt
     1801 cctgcatcgc ttgttcgaaa cgctcgcgcg gaacagtctc ttcctcgaaa accggcagcc
     1861 ggtgggtggc gcgtccgagg acaaggcggt tgcccgccgc atcgcgccat tgcgcgcaac
     1921 actggttgcc gtaccaaagt tcaaacggtt ggaaacgatc ctcgcgcgac agcacggtgc
     1981 cgcggacaga caaaaaggcc tgcgcctgcg gcatcggcac gggatcggcc cgcgccgaat
     2041 cgagccccgg cagtttcagc ccaatgttcg gatagttccg gggggccgta aagcgcgccg
     2101 ccgcgccgaa agcggaaagg cacgtcaaca agagcgcggc accggacaga aagacacgta
     2161 acataatcgc atcatagcac atacgtggcg tccggataac cgtaaaaaga gggagctcac
     2221 ttgtcaaagt aaatacaacc accccagtcg catttacctt gacaagtgag tcccgtaatc
     2281 ggttcgtgat cgggggcatg cgagtcgagc gctgttttat gtgtgcgctg tcggaaacgc
     2341 gagcgtcttc cgcgctacgc gcggatttag gatttattta ggatttagga gttaggagtt
     2401 aggagttagc ccgccgcgcg gctctgccgc gaaaaaacct tggaagtacg tttagggcat
     2461 gattaacacg acgttgttca atcctgaaaa tcctgtaatc ctggcaattt ataggacatg
     2521 attttccgct gcccgggcgc atatatccat gattaacatg atgttgttca atcctgaaaa
     2581 tcctggcatc ctggcaattt ataggcaagg agtttttcac ttcgacgttc tgagttcggc
     2641 gttgggcgtt cggcgttcaa aggcggcggc gtgaaacgcc gcccggacga gcagacgctc
     2701 gtccctcccg ttgacccgcg actggcgacc cgcgacctgc ggtgtcggga atgcgggcat
     2761 ccccgcccgt cggtagggcg gtttccccga aaccgccgcc tcgcaagcaa acaaacgtcg
     2821 cgggtcgaat gtcgcgggtc gcgggtcaac gtacacgaaa acgtgtacga ttccgatagc
     2881 gatcccgata gcgatagcga tttggatgaa accctgaaac catgaaacca tgaaaccaaa
     2941 gaaagtgtcc cgtggttaaa tgatcgatcc cgatagcgat cccgaccccg atcccgattt
     3001 ggatgaaacc atgaaaccag aaaccttgaa accctgaaac caaagagagt tgaaggttca
     3061 actgctcttt ttagggtaaa aaaagcgttc ccgtcttgcg cggggagaaa cacggcttcg
     3121 gcacgccggg ctccgcgccg atggcgcgta gctgcggacg cccgggggga tggttttgat
     3181 ctccccaaac catgccgatt accgtaacct tattcatcgc tgtccccctt ttgtgtttat
     3241 gcctttgagc gcgtaaagga aaagccatga gcaaaaaaat ctatatctct gccaacgcct
     3301 acctgttgga cagtttccgc ctcgcacggc gtatcctcga ctccggctgg ctaccggatg
     3361 acctgatcgt gctctggcgg ggcggggctc ccgtgggtat ggcaatccac gaattcctct
     3421 cctaccacgg tctgcggcca cgtcaccgga cactgaaatg ctattcctac accggcatcc
     3481 gaacccaccg cgaagaggtc gctttcgaaa acgccgacgc ggtcttcagc tccgtcaccc
     3541 cgggttcgcg ggtgctggtt gtcgatgacg tgttcgatac cggcaacacc gcgcgcgccg
     3601 tgctggatcg gctggcgccc tctggcgcgg agacgcggct ggcaacggtt tattggaaac
     3661 ccaaccggaa ccagacctcg ggtaaacccg acttctatct gcatgagacg gatgagtgga
     3721 ttgttttccc gcacgagctt gagggactca cccccgcaga ggtgcaccgc aaagaccccg
     3781 cgctccacgc gctgctgaaa tcgcccgcgt ctcagccgcg ctgacggtac tcccgcatgg
     3841 tgaggccgaa ctcgcgcttg aacaggcgcc gcaggtgatc gtcggtctga tagccgctcc
     3901 ggctggtgat ctcgctgacc ggcaggtcgg tctccgcgac aagacgccgg acccggtcga
     3961 gccgcaccgc gcggatcgcg tcgagaatcg agtggccgca ggttgtccgg aaccgtagct
     4021 ccgccaaccg tcgggagacc ttgagatacg cgaccacgtc ccgcacaccg atcccctcgc
     4081 aggcgttgag gcggatgaac tcgagcgcgc gggccaccag ccggtcctcc atgtgcggca
     4141 cctgccgcgt cgagtgccgc tcttcgatcc gcttgacccc gtaggtcagt tccacccgct
     4201 tccggtgccg accgcgcatc tgctcattca gcaaccgggc cgccatgaaa ccacagtttt
     4261 ccatgtcgca ttcgatgctg gagagggtcg gaaaactgcc gaggcaaagc tgctcatcgt
     4321 tgtctacccc caagatcgcc acctcatcgg gaactttgat gcctgtgatc agacaggtgt
     4381 cgagcacctg ccgcgcgcgg atgtccatgg cggcgaagag cgcgaccggc ttcggcagcg
     4441 acgccagcca ttccgccagc cggagttgtt ccacagccca gtcgcgaatc tcggctcgcg
     4501 acggcggcgc gtagacctga cacacacggc cgacctcgcg caaccgtcct gcgaaacctt
     4561 cgccgcgccc cttggaccat aaccgcccca tcacttcgcc aacatacgcg aaccgttcaa
     4621 atccgcgttc gaggaaaaac tcggccgcag ccctgcccac gctgaccgcg tcgttctgaa
     4681 ttcggcaaca gtgccggaac ggcgagtccg gctgcagatg acgcggcaag ggatcgatca
     4741 gcacgatcgg cattttggcc cgggccaggg ttcgctccat atccagcatc gactgcccca
     4801 cgatcacgcc gtccgcatgc cacgccccga aatgcgccaa ccgctgttcg cccacacggt
     4861 tctggatgat gtgcagatgc cacggcccgt aaaaacggac ataccggaga atcccgttca
     4921 acttctcccg gtccggctgc acgcaggtct cgagaatcac gaccacccgc ggggccgcag
     4981 tgaacatttt cgccatgtcg tccccttcct tccgtaccat gaagtatgcg acgacaacgc
     5041 gcatcgtgca agcgcgtttt gcgcaaaatg ccggatcagg ttatgcgcaa accgtggtgt
     5101 tcatttcgcg tccggacctg tatactgttt tttgagtaga gaaatgccaa tccacgaccc
     5161 ctgaccggaa aggaacctca tgacccgcta ccgccagtcc gttttcctgt ttctcctgcc
     5221 gttctgcgtt gtcgccgcga atacgccgac tccggacctc gtgctggccc gcaagggacg
     5281 gcccgccgac cgcgccatcg tgtgcccggt tcaggcatca ccctcccaac ggtatgcggc
     5341 cgaagaacta cagacctttg tggagcagat gaccggcgtc cggctgccga tcacggatga
     5401 tcgcgcaccg ctgcccgcca aggcgatcct gctcggcgac acgcgccaca ccgccgccgt
     5461 gctgggcgca acgcccgcgc tggacggcct cggcacagac gggtttctct tgaagaccgt
     5521 ccccccgcat ctcgtcattg cggggagtcc ggtgcgaggc acgctctacg gggtctatga
     5581 gctgctcgaa cgttacggcg gatgccgctg gtacaccgca gactacagtg tcattccgaa
     5641 acgcgacacc tttgccatcc ccccgctgaa tgaccgccag acacccgcct tcgcgatccg
     5701 cgaggaccgc tggaccgaca tgatccccgg cgacgtcgcg gcccgcaatc gctgcaacgg
     5761 ccacgacatg ctgcttgagg aacgccacgg cggcaaggtg cgcttcggcg cgggtctggt
     5821 cacccacacc ttttactcgc tcatgccgcc ccgcgaattc tttgcggagc acccggaata
     5881 ctacagtctg atcaacggca aacgccgcgc agagaacgcc cagctctgcc tgacgaatcc
     5941 ggatgtcctg aagatcacca tcgcacgcct gatcgagcgc atccgcaaag atcccaacgc
     6001 gcagatctac agcgtcagcc aggaggacaa tcgggcgtat tgccgctgcg ccgcctgcga
     6061 ggcgatcaac cagcgcgagg gcaccccggc gggctcgctc atcgcgtttc tcaatcaagt
     6121 cgccgaagcg gtggagaagg tctatccgga tgtgatgatc gataccctcg cctaccagta
     6181 tacccgcacc ccgcctaaaa cgctcaaact gcgtaaaaac attctgatcc gtctctgcac
     6241 catcgagttc gacttctcct tgccggttaa ccagagcacc aaagcatcga acaagcggtt
     6301 catcgacgag atccgcggct ggcaggcgct caccgacaat ctctacatct gggactatgt
     6361 caccaatttc ggcagttatg tgggggtcta ccccaacttc ggcgccctgc agggcaacct
     6421 ccgcttctac cgcgaccacg gcgcggtggc catcaaacag cagggtgcgc gacccggcaa
     6481 gcacgcggac ttcgccgagt tgaaggcatg ggtcatcgcc aaactgctct ggaaccctga
     6541 ccagccgctc gaaccgctgg tcaccgattt tctggaggga tactacgggg cggccgcgcc
     6601 ccatgtccgc gcctacctcg acgcgacgac ccgtctggtt gccgatccgg acattcacat
     6661 tggctgtttc aacagacccg agacatatcc ttggctcacc gatgacctgc tggcaaaatt
     6721 cgcggacacc ttccgccagg cggagcaggc cgtacgcaat gatccgccgc gcctgcacca
     6781 cgtccggatg ctgggtctcg gggtcctcta tacgcagctc cgccgcattc ccctgcccgc
     6841 cgcaccgcaa atcatctggg ccgccgacgg cgcgcgcctg aacaacatcc cgtccgccac
     6901 cgaagcgctt gcccgcgagg tggtcgcccg tctcgacgag gagccgggca tcctgctcct
     6961 tcaaaacagc ctgacgcatc atgtgatcca ggcgcaatac cgggctctgg ccggccgcgt
     7021 gccgcaccac acgtttagca acgacgccat caccgcctcg ctgaccgagc tggagaacgc
     7081 caagctgggc tggctcgcaa aaaccggcga ccccgcgttc aactacctct ccccgaaccg
     7141 ggactggggc gtgcagatcc agcccgacca gcaggaacga acagccgccg agcttttcgg
     7201 gatggtttac aggaaggcga aaacctctca ggatgccccg aggttcgccc tctcccgact
     7261 cggcgcgatc tccagcgagc gccatctcaa aacggaaggc tcccgcctgc tattctccgc
     7321 acgcttccgc aacacgcggc agaccctgca acaggaaacc gagccgatga tccgtgccgc
     7381 gctcaatctc ggccaacacg gccgtttctg ctggcgcacc gctcccgacg ccccctggcg
     7441 cacacagagt gaattgcgcg aacccgttgt cgcgctctcc gcggccgaac gcgggaccgg
     7501 ccaaatcgag attgcctcgc ccgttacgcg ccgcatgctg cgcctgacct ttgaccccgc
     7561 ccgcgcggtg tccgtcaccc tgcgtctgca tcaggccgaa gcggaaagcg tccgtgtcca
     7621 gatcacggaa aaaccggaaa cgctcgcccc cggtgcggaa cgggtctgtc ccctcacgct
     7681 ggagatcgcg gatccgcccc cggacctgcc gggcagcctc gcaaccgctt ttgtcacgcc
     7741 gcccgttggc cagccgccgc atatcgtctg gcagggccag cccgcggcgc ggatcgtagt
     7801 gccggtccgc gccacgccgg tcgagcgcag cgccgcgcgc gaactgacac tgcacctcga
     7861 agccatctgc ggtgccgctt ttgaaaccgt caccgaggac gagcccaaaa ccgggccccg
     7921 cctgctggtc ggcaataccg atgcggcacg gcgtctgatt cccggtttcg atccggcttc
     7981 cctcgcctat gacggcatcc tgctcaagac cgcgggcgat gacctgatcc tcgccggcca
     8041 cccgcaacgc ggcccgatct atgccgtcca cacctttctg gaagattccg tcggcgtgcg
     8101 ctggtggacc tcggacgaaa ccttcctccc gcaacacacc agcctgccgc tgcccacgct
     8161 cgatctccgt tacgaaccca aactgaaatt ccgcgaaacc tactatctgg attcgttcaa
     8221 cgcccgcttc aaggtacgca tgaagggcaa tttcagctcg cgcacacgct ttatgctcga
     8281 cccgctcgag atgatccccg aggcctacgg cggcaaccac cggctgatcc acttcgcagg
     8341 gcgcaactcg gcctaccact cgttctacga actgctgccg cccgccaagt atttcaaggc
     8401 ccacccggag tggtacagcg agatcaacgg caagcggacg cacacgcgcg cccagctctg
     8461 cctgacgaac gaggagatgc gccgcgaact gacgaagaac gcgctcgccc tcctgcgcac
     8521 cgaccccggc gcggacttta ttcagatcag ccagaatgac cacgctggcc gctgcacctg
     8581 cgcgcgctgt ctcgcggtcg aaaaggagga gggcggtgtc ggcactgcct ccgggccgct
     8641 gctccggttc gtcaaccaag tggccgaagc gatcgaaaaa gagttcccga acgtctggat
     8701 cgacaccttt gcctaccagt atacccgcaa agccccgaca aaaacccggc cgcgccggaa
     8761 tgtgctggtg cgcctctgcg acattgaatg ctcgttcctc catcccatcg agggtacgcc
     8821 gcagaacgcc aaatttttga ccgaccttgt ggagtggagc cgcatcgcgc cggggaacct
     8881 cttcctctgg gattacgtca ccagcttcag ctcctacatc ctcccccacc ccaatctgcg
     8941 cgtcttggct ccgaatgtcc ggacgttcgt gaagcacggc gcgatcggtc tcttcgaaca
     9001 gggcgacgcg ctctgccgcg cgggtgattt cgtgcgcctg cgccaatggg tgctcgcgca
     9061 cctcctctgg aaccccgacc tcgacgcgga cgcgctgacc gacaccttcc tcgccggata
     9121 ctacggaccg cgcaccggcg cggccctgcg ccgctacctc gattttctcc atgatcgtgc
     9181 ggcgcgcgcc gatgtctacc tcggttgtta tcagaacaac gtcacccact ggctcgacgc
     9241 cgaaggcatc ctcgaagcgc tccgactcat ggatgcggcc ctggccgaag ccaaggccga
     9301 agagcaggcc gatccggtcg gacggaaagg tctggcggat aaaatccgtc gcgaaaaact
     9361 ccccatggat cacgtcgtga tcctcaaccg tcccctgctg caacgccatg ccaaggccac
     9421 cggcaaaccc ttccccgtaa ccgcggaccc gctggccgcg gttgaagcct ggatcgccgc
     9481 ctgcgaacgt tttggcgtca aggcgcatcg cgaaaccacc acggccgaca ccttcatcga
     9541 ctacaagaag agcctgctgg aacgcgcccg catggcttca caaaaggtca gcccgccccc
     9601 ctgttgcgca accctgcctc ccgcctcctg gcacgatgtg caggagttcg agttcgatca
     9661 gcggcccgca ggccaagttg ccttcgtgga ggacaaggcg gcgtccaaca agatggccgc
     9721 gcaaatgccc ggcaaccaca agatctgggc cgtgcagttc ccgctgacgt ccttcatacc
     9781 cctgtccgga acggcgtccg gcacgccgcg ttgcacactg cacgtctacg cgcgcgccga
     9841 tgcgaccgca accgagggta acggactcgc ccttggcgtc tatgatttca cggcgcgaaa
     9901 aaccgtggcg caccagtgga ttccgctgtc cgaactcacc ggccccggct acaagatgtt
     9961 taagttcggt cctctgccga tcacggaggg ctgtaaattc tgggccaatc cgccggaacg
    10021 gacgggcgag gttcagcacg tatacgtcga ccgcgtcgtg gtcgtggtcg aataaacgcg
    10081 cataaagttg acgcggataa acactccgca tgaggaaagg agtcatcatg aaaaaaatgg
    10141 tgttcatcgt aaccgcagga ctcagcgcct gcctgcttca cgccgcctca tgggttccta
    10201 cccagccgtc tccggacgaa gcggccatcg cctgtatcaa cacgtccgtc acaaacggaa
    10261 ccgatgtgga catcacggca accctcgcca gcaacacggc cgaggtggtg atcggcacgg
    10321 ccctcagtga ccgccacacc ttcctccgcg ccgtggacct cagcggacgc accggcacgg
    10381 gcctcggatc cgccgacaac tcggccttcc atgcggctgg cgtggacgat gccgcgaccg
    10441 gtgccgccat cctgctcaaa cggttgagcg gcgatgtaca cggtgcgggc gtcacccacg
    10501 ccttcggtct gctcgcctgg tcggacggcg ccctcggcaa caccccggcc accctccaga
    10561 tcgatcacct cacgggaacg gtgagcgcca acgtccaccc cgacggtacg gctgcggccc
    10621 tgtacgcgcg caccgccctg cacctcaacc tcatgcccgg cgcactgctc ttcggcggca
    10681 gctacggatg ggacctcggg gaccagaccc tgaccaatgc ccaagccgaa ctggtcggcc
    10741 tgctcgccaa agtcagggag ggcgccgcaa ccgcgtcgga aagcaacacg ctgaaaacag
    10801 ccgcttccgg atatgccatc cttggcggcg aagaggacga cttcctccgg ttcggtggtc
    10861 tggccaccct cttcggtcag gtggacctgg gtgacggcat ggatgtggtc tcgctctgcg
    10921 acctgccttc caacacggtg acgcgcctgc ccgcgatcgc gaacgcggag ctgctgctga
    10981 tcaccaactc cccgatcttc tcggcctccc ttgccggcgc tcccaaagaa accgtgatcg
    11041 cgggcgactc cttctgtaca ttcgttgacg agctcaccac gctggattcg ctctcgggaa
    11101 ccggcacgct ggcgtcggaa gggacgatct gggtcaccaa cctgctaagt gtcagcgggt
    11161 ttctcgccgc accgaccggc tcggcgctca tcatcgaagg aaacctgggg ctcgccgacg
    11221 ggcttgcctg ttccctgcgc gcctgcggca ccgcagacgg atggaccaac gaccgcatca
    11281 ccgtgacggg aaccgtgacc gtcgacggcg gcggcaccgt ggatctcggc tgcacgccct
    11341 ccgacccgat tcccgaaaag gagcgccgaa tcctcatgac cgtcgcgggc ggggtcatca
    11401 actccgccaa cctcgcctcg tggactctga ccggcacggg atgggatgaa gatcccagcc
    11461 tgatccgccg gatcggtaca gacggagtta ccatttatgc gtccgtctta aaaggcggca
    11521 cactcctact gatgcgttaa cgacctttct ggaggaacaa cccatgaaaa atctttccaa
    11581 gcctctcgca atcttctcga tgaccgcctg tctggccggc accgtgcatg cgcagcagat
    11641 tctcactacc gtgcagaaca tcaccgccgc tcccgcgccg gccatcactt gggccaccca
    11701 acccgacggc gtgtattcgc ttctcgcttc ggacaccgag ggcggaccgt tcgacacggt
    11761 cctgatcgcc tccacgcaga ccgtcgaaaa cagcatcact tacgtggact ggcgtcaggc
    11821 aacggaaggc acgcggcgtt actaccgggt ggtggaaaac acaccgggat acgcgaccta
    11881 catgatcgtc gacctctccg gggggccgga tgcgacggtt taccccgtgt cttataccaa
    11941 cgccgtgccg gacctgctca cggacagcgc gtacaaaacc aacaaaatgg tcttccgcct
    12001 gattcccggc ggcagtaaca tcaccggctt ccccgaggat gtgccgcgcg ggcagtatac
    12061 cccctacatc cccttaggca ccatgaccct gacccggccc cattacatgg gcgtgttcga
    12121 ggtcactcag gaccagtatg ccctggtgat gaacaccaac ccctcgccct ccaaccgctt
    12181 gggagcgacc cgtccggtgc aggatgtgac ctataacgac atccgcggtt cggccgcgca
    12241 gggcggcggc ggctggccga gcaacagcga ggtctacacg aactcgttca tggggcgcct
    12301 gcgccggaaa accggatacg aagagttcga cctgcccact gcgggccagt tcgaattcgc
    12361 cacccgcgcc ggaacgacgg ccctgtttta caacggtttc aacgacccga cgaacacaac
    12421 cgacttctcc ggcatcacca atatcgcccg ctgctgtggc aaccagttgt cgacatgggg
    12481 cggatacact tacgcggcct cggtgggcag ttacctgccc aatgcctggg ggctctacga
    12541 catgaccggc aacctcagcg aacactgtct ggattggggc ggtaggaact caggcaccga
    12601 tcccgtcggc ccgcagagcg gcacttcccg taccgtgcgc ggcggggcgc acagcacgag
    12661 cgccctctcc acactctacg ccggagcgcg ggactccatg aaccccggca aggtcacgca
    12721 gagcggcgtg ttcggattcc gcaccgccct gcgcctgccc gacacgccgt ccgaggagat
    12781 gcgctcttct gtctcggcag gcaccatcct ctccgtaacc ggaacccccc gtcccgcttc
    12841 ccggatcacc tggcaaaccg tgcccggtaa aacctatatc gtgctctgcg cgacaaacga
    12901 gacggggccg ttcgaaaccc agatcggcga cacgctgacc gcatccggca acacgctcag
    12961 cgtgacggat accggctaca cgcccgatgg accgcagcgg cactaccgcg ttgaagagtt
    13021 gcccggcggc tatgcacggt accggatcgt ggacatctca ggcggcacca acgccgcatc
    13081 gtggccggtc acccacacca acgtgctgcc ggacatcaac tccgccctct accggaccac
    13141 ccgcatcgcc ttccggctga tcccgcccgg aagcttcacc atgggcgcgc cgctctccac
    13201 cgtgggcgcg accacaaaaa ccacgcccca caaggtcacc ctcacccggc ccttctacat
    13261 ggccgtgttc gaaaccacag aggcgcaaca ctcgctgatc tatggtgacg gctcgggcac
    13321 ctcgcaatat ccggtcggga acgtgaccta cgacatgctc cgcggcagta ccagtgactg
    13381 gccgctgagc agtaatgtcg cgccggattc cattatcgga atgctgcgcg ccaaaaccgg
    13441 cggcgcactg gatttcgatc tccccaccga ggcacagtgg gagtacgcct gcagggccgg
    13501 taccacctcg gcgttcaaca acggcgacga cttgaagacc gaaacggatt tcggtacgct
    13561 aaaagcaacg ctcggtttga ataacaggca tgcgggaggt gcctatcaac ccaactcatg
    13621 gggtctgtac gacatgcacg gcaaccttct ggagtgggtg ctggaccgtt ggcaggagga
    13681 cctcggcaca gcggacgtgg tggatccgaa cggcccctcc accggcacaa agttctctac
    13741 ccggggaagt gcctatatgg cgaaggatag ctttaagtac gcctcctttt cgcgggagaa
    13801 cgggggaatg aaccgctatg gctcaaccgg cttggtctcc taccggctcg cctgccgcct
    13861 tgactgaccg caacgcataa acaaaggaaa caacatgaac gcacatatga aaatcctcac
    13921 cgtcagcctc acgctcgccc ttttatgggg cttctcaggc tgccgtccgc cggagaccgc
    13981 gcgaacgccg cgcgatcagg cgcttgactt catccgcaac gaaacccagt tccacctcgg
    14041 ctacctgccg accgaacagt cacatcccaa aacgcgcggc ctctcgcagg cgttgcaggc
    14101 cgacaccgcc aaaggcatcc gcatgttgtt gagcgtggac gaggacatcc cgcccgtggc
    14161 ccgccgcgtg gccgcctcgc cggagttcgc cgaactgcgc accgcgatca agaccgcgct
    14221 ggacgcgaac aaaaaagtct acttcagcgg ttgcggcgcg accggacggc tctcaatcct
    14281 gcttgatgcc gccaaccgcc gtttctggcg cgagacctac gaacgccacc cggacctcaa
    14341 agccgtgtgc ggcgacatgg ccgggcgcac ccacgcggtc atgaccggag gcgattacgc
    14401 gctcgtgcgc tcggtcgaga tgttcgagga ctacatcacc ttcggctacc accaaatgga
    14461 gcaggcgggc gccgcagagg gcgatgtgat cgtggcgatc tccgaaggcg gcgaaacctc
    14521 gtcggtcatc ggcacgatcc tgcgcggttt ggacgtcaag gccaaagtct tcttcctgtt
    14581 caacaatccg gccgacctgc tggccgataa gctggagcgc tgccgccggg tgatccgcaa
    14641 cgacgcggtc accaagctcg tgctctgcac gggccccatg ggcgtggccg gctccacgcg
    14701 catgcaggcc acgaccatcg aaatgcttgt cgcgggcatg gcctatgagg ctggcctgaa
    14761 agaacatctg cagaccaagc tcaactccac ccagatcgcc tcgctcgggc tcgatgcgcg
    14821 tacgcccgaa aagacgctcg aacagtttga cacgctgctg gggcagatcc gcagcgagcg
    14881 gaacgtgacc gccctcgcgc gactggcgga ctatgaagaa agtctctaca cgcgcggcgg
    14941 acgcatcacc tatttcgcgg acggttatct gctcgatatc ttcaccgata ccaccgagcg
    15001 tgcgccgacc tttaagattc cgcctttccg acgttgcgac gacaccctct cccccgcgcc
    15061 ctgggccttt gtcaaagacc ccctgcgcac cacaccggag gcgtggctgc gcgtgttgga
    15121 gcatgagccg cgctgtctcg actggtcgcc cgaaacctac atccggctcg gtgcctcacc
    15181 cgatatccgc aagaacccgc cgagaatcga ccgcgcctcg ctccacacgt tcctcatcgg
    15241 gaatgagccg gacgcctcgc gtaccgagat ccggcccaac gccgccatgg ccgtgctgat
    15301 cggcg
//
LOCUS       DAOSRB010000050        15174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4009_length_15174_cov_5.901978, whole genome shotgun sequence.
ACCESSION   DAOSRB010000050 DAOSRB010000000
VERSION     DAOSRB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15174
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4009_length_15174_cov_5.901978"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLZ74_04290"
     CDS             complement(<1..547)
                     /locus_tag="PLZ74_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR97574.1"
                     /translation="MMKFVAVFALMASVVIGADFQHTLSGVKPWTSEEFLGGPQEFHF
                     AVLPDRTGGERKGVFGKAMDTLNLLRPAFVMSVGDLIECGGNPDAREQWAELETHISK
                     LDMPFFYVAGNHDIWTGLKGMTPKRQKSIDLWHELHGTNTYYSFTYKGCLFLCFNTME
                     KHDYYPPREPLPENQIAWALEE"
     gene            840..1814
                     /locus_tag="PLZ74_04295"
     CDS             840..1814
                     /locus_tag="PLZ74_04295"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOR97575.1"
                     /translation="MKAKIKLIVVATLAAFNLFSNADAIKVATETKPLKVLMIGNSFS
                     DCVLRQAPQIAQNLDCPLNITSLYIGGCPLSRHAANLDTTNAPYMVSWSYCGNSKREA
                     VPFAKVLKTRKNKDGKQRPNRFGNLRQMIAADKWDIVTIQQASHDSWRPESFQPHADK
                     LIAAVRELAPQAQFRIQQTWSYCTADKRIHNPETNGPGTWGFDQTGMYERLTANYTKL
                     AKDTGFAIIPMGLAVQKFRKAKGIKSHEGDVVGNIRMENGKATGDTIHLNGAGHYLQG
                     LVWVGTLFDVDVTKCTYAPKNMDPELAKVLRACAAAAVAETRGVQTKK"
     gene            complement(1820..3322)
                     /gene="glpK"
                     /locus_tag="PLZ74_04300"
     CDS             complement(1820..3322)
                     /gene="glpK"
                     /locus_tag="PLZ74_04300"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOR97576.1"
                     /translation="MKKTSLILALDQGTTSSRAILFDRELKIVATAQQEFGQFYPQPG
                     WVEHDPEEIWRSQRKVMADVVKQTPDGPAAVAAIGVTNQRETVVVWNRRTGEPIYPAI
                     VWQCRRTADFCSELKRRDYGPRIRDKTGLVIDAYFSASKLRWILAHVDGAREAAQRGE
                     LLCGTIDSWLLWKLSNGTAHVTDYTNASRTMLFNIRTLQWDAELLDLFDIPSSMLPTP
                     VPSSGEAARCVLPEFEGREVPVLGVSGDQQAALFGQGCFAPGQIKNTYGTGCFLLMHT
                     GGEAVSSANGLVTTLTAQTLAGAPSYALEGSVFVGGAVVQWLRDELCILTQASDVGPI
                     AASIPDTGGVFLVPAFAGLGAPHWDMYARGALVGMTCGTGRAQIIRAAVESIAYQCRD
                     VVDAMRLESGQPIASLRADGGASACDFLMQFQADLLECRVERPTCIETTALGAASLAA
                     LALGWQTPETLAGRTAASVFTPSMPETQRNALLAGWRKAVSRAARWETES"
     gene            3575..4867
                     /gene="murA"
                     /locus_tag="PLZ74_04305"
     CDS             3575..4867
                     /gene="murA"
                     /locus_tag="PLZ74_04305"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOR97577.1"
                     /translation="MSRFIIKGGRPVSGVHPVPGNKNAALPMLAASLLTAEPVVLSNL
                     PLIADVRTMIDLLVELGAQVELDRARRTVRICAQRIKDTHLNKTLCGKVRSSILFAGP
                     LLARCGSAKLYPPGGDVIGRRRVDTHLDGFRQLGASVRAGRTYLFKAAKRLEGQRILL
                     DEASVTATENLIMAAALARGTTLLYNAACEPHVQDLCRMINAMGGRISGIGTNLLTIE
                     GVESLHGTKQRVGPDYIDAGSYMVAALVTGGELTVEGVEPSDFEALERPFKRLGAKWS
                     FDREARSLRLPARQRLKTAYDLGDAIPKIEDGPWPMFPSDLMSVLIVLATQTRGTTLF
                     FEKMFESRMYFVDHLIGMGARIVQCDPHRVVVTGPTILHGTTVASPDIRAGMALLVAA
                     LCAKRETVIYNAGSIDRGYESVEVELSRLGAEIERVPT"
     gene            complement(4901..5782)
                     /locus_tag="PLZ74_04310"
     CDS             complement(4901..5782)
                     /locus_tag="PLZ74_04310"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOR97578.1"
                     /translation="MRACVLVAPTVGAGPRERLNAALARVSDAHGMAVEVLDLPAADH
                     LADEIRAILDRGVECVVAAGGDGTVAAVAQVLVGGGVPLGIVPMGTGNLVARELGIPL
                     EVERAAERLACPSSFRRLDVMRINGRIYLLNAGVGVNAEVIDRTSRRGKTLFGVSAYV
                     GTAVWKVLQARPHRLEIVIDGVSMVYDATDVLISNCGILARGLYPNGPDIRADDGRLD
                     VCVVCMKRPIEYPWSYVMRLLRMRNVNRVLHEHAAACAVTVRSARPLVVQADGDIIGS
                     TPVTVEILPHALSVIVP"
     gene            complement(5779..6579)
                     /locus_tag="PLZ74_04315"
     CDS             complement(5779..6579)
                     /locus_tag="PLZ74_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR97579.1"
                     /translation="MTRAFLKTLIRRYRAYVDSFRDARGGLSELHRLKIEHTERVAEN
                     ACRIMSGENWPEAWTAAGEACAWLHDIGRHVQLRDYGTFRDADSLDHAACGVAVIREE
                     RLLDGLAAASRARILAAVALHNRKTVPDGLADDAARLTHLVRDADKLDIFRVLEEVVD
                     DGSLRRNPAIAWGLRLDGAPSPDVVAAVCAGQPVSYDAVRNLSDFVLIQVGWLINGLH
                     YTASLRLAAERRVLEYREAFLKTLSTDAGVTRCCDAARRHFAAKAVGA"
     gene            complement(6576..7493)
                     /locus_tag="PLZ74_04320"
     CDS             complement(6576..7493)
                     /locus_tag="PLZ74_04320"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOR97580.1"
                     /translation="MIPTRAAIWFRALRPAQWLKNGVLPAAYFFARWDPTQCEGVRGV
                     LPLVYVALAVACFCCVSSGIYLINDVRDIEADRAHPLKRLRPLAAGQIRASAAVAVAI
                     VLLAAGLAAGALTLPRAFTEVLLAYGLMQLAYTFGLKRIPYVDVFVIAVGFVLRAIAG
                     AAALSVRISPWLLLCTFLLALFLALCKRRHEKVLLDEDGERHRKALAGYDRYLLDLQI
                     AVTASATLVCYAMYTLSEETVRRFGTNRLGFTIPFVVFGLFRYLELVYRHDGGGRPEQ
                     TLLTDRVLIGTMILFLLTALAVFLTGQTV"
     gene            complement(7490..8239)
                     /locus_tag="PLZ74_04325"
     CDS             complement(7490..8239)
                     /locus_tag="PLZ74_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOR97581.1"
                     /translation="MSEKTLIIIPTYDERENVEPMSEAVLREVPTANILFVDDHSPDG
                     TGAVLDAIAAREPRVFVMHRAGKEGLGRAYIAGFKWALERGYELICEMDCDFSHDPAA
                     LPVLLQTAREGADLVLGSRYIGGIRVMNWPMSRLLLSTGAAQYVKLITGMPVNDPTGG
                     FKCFRRAVLEAIDLDRITSNGYSFQIEMTHTAWMLGFTVREVPITFEDRRSGYSKMRA
                     NIAYEAVAMVLRLWGRCGFRRSPRPRPREET"
     gene            complement(8288..9367)
                     /gene="queA"
                     /locus_tag="PLZ74_04330"
     CDS             complement(8288..9367)
                     /gene="queA"
                     /locus_tag="PLZ74_04330"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOR97582.1"
                     /translation="MLTSAFDYDLPPDLIAQEPPAVRGTSRMMVLHGSDGRIEHRHVM
                     DLPAYLGADDLLVLNDTRVFPARLQGFWTDTHGALELLLLEPCPAPEGTACAEGTVCW
                     ECITGSGRPIRPGQRAVFADGQIEAEMLARQPNNAWRIAFRSPVPLMTLLERYGVTPV
                     PPYIHRCGDARQARLDRERYQTIYARETGAVAAPTAGLHFTEALFAALAARGVARAAV
                     TLHVGPGTFKPVKAEQVEEHRMDPERYAVPAATAAAVATCRARGGRVVAVGSTSVRAL
                     ETVAAAHGGEVTAAVGSSSIFIYPPYRFRVIDAMLTNFHLPKSTLIMMVAALAGRERI
                     LEAYREAVRERYRFFSYGDCMLILP"
     gene            complement(9383..10981)
                     /gene="nadB"
                     /locus_tag="PLZ74_04335"
     CDS             complement(9383..10981)
                     /gene="nadB"
                     /locus_tag="PLZ74_04335"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOR97583.1"
                     /translation="MMRIESCDFFVVGSGIAGLMSALYLAPHGRVLLVTKKRIDDCNT
                     NRAQGGIACVMAAGDSFEQHVSDTLTAGAGLCDEEVVRKIVSTGPARIADLEALGVCF
                     NPRTDGVPGYDLGREGGHSQRRVLHAGDITGQHVEAVLVEQILKHPHIQVLEHTMVID
                     LITTRWLKRPEENRCVGAYVLDQLAGEIYAVRAADTILATGGCGKVYLYTCNPDIATG
                     DGVALAWRAGATIENMEFIQFHPTCLYHPQAKRFLISEAVRGEGAELVDAEGRPFMQK
                     YDPRGALAPRDVVARAIDSEIKRTGAPYCCLDIRHKSRAFLQKRFPTLYKTCASFGID
                     MAHDLIPIVPSAHYCCGGIRASVDGVTSLPGLYAVGETACTGLHGANRLASNSLLEAL
                     VCAYETAGRIAAQPGRLPDAIRIPDWQYGTAVASDEAVMVAHNWMEVRTCMWDYVGIV
                     RTNKRLERARRRLRNLRREIREYYFDYLVTADTLELRNLALVGELIVRSAVRRRESRG
                     LHYTLDYPETKRFARNTSLPGRNA"
     gene            11297..12454
                     /locus_tag="PLZ74_04340"
     CDS             11297..12454
                     /locus_tag="PLZ74_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97584.1"
                     /translation="MRILKMGQVGGGIGAFIGEVHRKAARMDGGIELTAGAFDVVPSK
                     SLRQGKNLGLDPKRVYSTYEEMIAKESALPPGERVDFVSVCTPNHTHFPIAKACLKAG
                     FHVMCEKPMTLTVAEAEELAALVKSSGKVFGLMHNYTAYPMVKLARDMVKQGDLGTLR
                     KVVVQYPQGWLYQLAEKSGNMQASWRTDPKTSGVAGAMGDIGTHAANLAEYITGVKIT
                     RVLADLTAFVKGRKLDDDGNVLLRFQKGVKGVLHASQISVGEENNLAIWVYGDKKGLW
                     WRQENPNELHVLCQTAPEEIWRRGNGYVAEKSKAAARGTRIPSGHPEGFLEAFANNYC
                     NFADTIRAKAEKRKPSPEELDFPSVEAGVRGMKFIHAVVTSSKKGSVWVKI"
     gene            12542..13402
                     /locus_tag="PLZ74_04345"
     CDS             12542..13402
                     /locus_tag="PLZ74_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR97585.1"
                     /translation="MWSFSSAFSLPAIGLVLTLPFATLQAQQPGVTNNIAYYDDDAPQ
                     TDDAYRQSQCRLDLRYPVSKTGFATVVWFHGGGLTSGRRHFIDLKDPSIAVAAVGYRL
                     SPQVEHPGYLEDAAAATAWVLRNIGRFGGDTHKVFVSGHSAGGYLSAMIGMDPRWLAR
                     HGLSHRQLAGIVPISGQVTTHFHVKKLRGDTQSQYRPIIDEFAPLYYSSAELSPICLI
                     VGDRRIEYKNRVEENDLLATSLRNLGHQHVEFYEMGGLNHSTVGQGAMILLREFVGKL
                     SRPSDAAKTK"
     gene            13440..14165
                     /gene="pflA"
                     /locus_tag="PLZ74_04350"
     CDS             13440..14165
                     /gene="pflA"
                     /locus_tag="PLZ74_04350"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOR97586.1"
                     /translation="MNPQVTGRIHSIESFGTLDGPGIRYVLFLQGCPLRCLYCHNPDA
                     GDCEAGRVITAGEALTDILTYRAFIARGGVTLSGGEPLLQPEFVCALLEGCREQCLHT
                     ALDTSGAIPLSISRAALDRADLILLDVKAIDDDLCRTLTGRGNRETLATLEYCEAAGR
                     EVWVRHVLVPGYTLDPAHLTRLADHLLRYACIRKVELLPFHKMGEYKWEALQRPYRLS
                     DTPPPTEEEVQAARAIFEQRGLL"
     gene            complement(14229..15062)
                     /locus_tag="PLZ74_04355"
     CDS             complement(14229..15062)
                     /locus_tag="PLZ74_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOR97587.1"
                     /translation="MQSLLNHLNQLQELVLIRDEHRTTGDGSHMERLNDSIDQLTDKL
                     PPEIKSLYQRLYKKDHIVMAPMYNGCCAICGMRLPISQVQSVRLCKQIQNCPSCARIL
                     FEESGAPRWVGDVPSRTEPRKSGISRFSAESLMIPDLKATNGREAIVELATVMEQQKF
                     VSGAEKLVASALEREAVLSTAMDNGLAFPHVRGVEGGGLTLALGVAKKGIRYDEAGSI
                     VNLVFFITIPTAVSAFYLRLMSGLTETFLKEQNREAMLAAETPELLWKALIKATRYTI
                     K"
BASE COUNT         3066 a         4913 c         4468 g         2727 t
ORIGIN      
        1 tctcctccag cgcccacgcg atctgattct ccgggagcgg ttcgcgcggc gggtagtagt
       61 cgtgcttctc catcgtgttg aagcatagaa acagacagcc tttgtaggtg aaactgtagt
      121 aggtgttggt gccgtgcagt tcatgccata agtcaatgga tttctgccgt ttgggcgtca
      181 tccccttgag gccggtccag atatcgtggt tgcccgcgac ataaaagaac ggcatgtcga
      241 gtttgctgat gtgcgtttca agctcagccc actgttcgcg agcgtccgga ttaccgccgc
      301 actcgatgag atcacccacc gacatcacga acgcgggccg aaggaggttg agggtgtcca
      361 tcgctttgcc gaacacgccc ttgcgctcac cccccgtacg gtccgggagc accgcgaaat
      421 ggaactcctg cggaccgccg aggaattctt cgctcgtcca cggcttcacc ccagagagcg
      481 tatgctggaa atcggccccg atcaccacgg acgccatcaa tgcaaaaact gccacaaact
      541 tcatcatgtc ttcatctccc tcgtttataa cgtgagcgtt ttgtcacacc tttcgggcgt
      601 ccgagacatc cgcccgtact gttcattgct caaccggtcc atgccagtac ggcgggacgt
      661 ccaaagctac gtgccgcagg cgcgggaccg gaccgcatcc gttcagacgc gcaaaaaaac
      721 gttcttccgg cattttatta tgcaacaggc tgaaaaaagt caaccccaaa ccccgtagcg
      781 ggatggatga gggtgtttat tgttgcggtg gatgtattta atcatgtacc atataaatca
      841 tgaaagcaaa aatcaagttg attgtcgttg caaccctggc tgcgtttaac cttttttcca
      901 atgccgatgc gataaaagtc gcaaccgaga cgaagccgct caaagtactg atgatcggga
      961 actcgttctc ggactgcgtc ttgcgacaag caccgcagat cgcccaaaac cttgactgtc
     1021 cgctcaacat caccagcctt tacatcggcg ggtgcccgct atctcggcat gctgccaacc
     1081 ttgacacgac caacgctccg tatatggtgt cctggagtta ttgcggaaat tcgaagagag
     1141 aagccgtgcc cttcgccaag gtgctgaaaa cccggaagaa taaggatggt aagcaacggc
     1201 cgaatcgttt cggcaatctc cggcaaatga tcgcagcgga caagtgggac attgtcacca
     1261 tccagcaggc gagtcatgat agttggaggc cggagtcctt ccagccgcat gcggacaagc
     1321 tgattgccgc agtccgcgag cttgccccgc aggcgcaatt ccgcatccag cagacatgga
     1381 gctactgtac cgctgacaag cgtatccaca atccggaaac gaatggccca ggcacgtggg
     1441 gctttgatca gaccggaatg tacgagcggc tcaccgccaa ctacacgaag ctcgcaaagg
     1501 acacgggctt cgcgatcatc ccgatggggc tcgcggtgca gaagttccgt aaggcgaaag
     1561 ggatcaagag tcacgaaggc gatgtcgttg gtaacattcg catggagaac ggcaaggcaa
     1621 ccggagacac catccatcta aacggggctg gccattacct acaggggctc gtgtgggtcg
     1681 ggacgctctt cgatgtggat gtcacgaagt gtacgtatgc ccccaagaac atggatccgg
     1741 aactagcgaa agttctccgg gcatgcgctg ctgctgcggt tgcagaaaca cgcggggttc
     1801 agacaaaaaa atagtttcgt taactctcgg tttcccagcg cgccgcacgt gacacggctt
     1861 tgcgccatcc ggcgagcaaa gcgttgcgct gcgtctcagg catgcttggc gtgaacacgc
     1921 tggcggccgt ccggccggca agcgtttcag gcgtttgcca gccgagggcg agggcggcca
     1981 atgatgcggc accgagcgcg gttgtttcaa tgcaggtcgg ccgttcgacg cggcattcga
     2041 gcagatcggc ctgaaactgc atcaggaaat cacaggctga ggcgccacca tccgcacgca
     2101 gagaagcgat cggttgtccc gactccaagc gcatcgcatc gaccacgtcc cgacactggt
     2161 aggcgatcga ttccacggcc gcacggataa tttgagcacg ccccgtaccg caggtcatgc
     2221 cgaccagtgc cccccgggcg tacatgtccc aatgcggtgc gccaagccct gcgaaagccg
     2281 gcacgagaaa gaccccgccg gtatcgggga ttgatgccgc gatcgggccg acatcggaag
     2341 cttgcgtcag gatacacagt tcgtcacgca accactggac aacggcaccg cccacaaaga
     2401 cgctgccttc aagcgcgtaa gagggcgctc cggcaagggt ctgcgcggtc agcgttgtca
     2461 ccagtccgtt cgcagatgaa accgcttcgc cacccgtgtg catgagcagg aaacatcccg
     2521 tgccgtatgt gtttttgatc tgtccggggg caaagcagcc ttgaccaaag agcgcggcct
     2581 gctggtcacc tgacacgccg agtacaggca cttcgcgtcc ctcaaactcc ggcaagacgc
     2641 accgggcggc ctcgcccgat gagggaaccg gggtcggcag catggatgac ggtatgtcga
     2701 aaaggtcaag gagttccgca tcccattgaa gcgtgcggat attgaagagc atggttcggg
     2761 aggcgttcgt ataatcggtg acgtgcgcgg tgccgttcga gagcttccag agcagccaag
     2821 aatcgatggt gccgcaaagc agttcaccgc gctgcgccgc ctcccgcgcg ccgtcgacat
     2881 gagcaaggat ccagcgcagc ttgctggccg agaagtaggc gtcaatcacc aaacccgttt
     2941 tatcacgaat gcgcgggccg taatcacggc gtttcaactc ggaacagaaa tcagcagtcc
     3001 ggcggcattg ccatacgatg gcgggataga tgggctctcc tgtccggcgg ttccagacga
     3061 caacggtctc ccgctggttg gtcacgccga tcgccgcgac ggcagccggg ccgtcgggcg
     3121 tctgtttcac gacatcggcc atgactttgc gctgtgaccg ccagatctct tcgggatcgt
     3181 gttcgaccca tccgggctgc ggataaaatt ggccaaactc ttgctgggcc gtcgcgacga
     3241 tcttgagttc ccggtcgaag agtatggccc ttgaactggt tgttccctgg tcaagcgcca
     3301 agatgagaga cgtttttttc atgacaggcc tcaacgttcc gctcacaccc gtttgcggat
     3361 tgattcgcac gcactgcggt ggcgccttgt cagcaccccg ccggtgtcca cattttccaa
     3421 tgtggggaag ttagcatgcg gtatcatgcc aagtcaacac gttaccgcat tcaaacaaaa
     3481 ccgaactgga cgggcatgag aaactggacg cgcaccgccc ttctatgtca caatgcgttt
     3541 gtttttaaca acccccaaga acggttgatt tcccatgtca cgattcatca ttaaaggcgg
     3601 tcgaccggtc agcggtgtcc atccggtacc gggcaacaag aacgccgcat tgccgatgtt
     3661 ggccgcaagt ctgctgaccg cagagccggt ggtgctcagc aacctgccgt tgatcgcaga
     3721 tgtgcgcacc atgattgatc tgctcgtcga actgggtgcg caggttgaac tggaccgcgc
     3781 gcggcggacg gtgcgtattt gcgcgcaacg tatcaaggac acgcacttga acaaaacgtt
     3841 gtgcggcaag gtgcgttcct cgatcctctt cgccgggccg ttgctggcgc gctgcggctc
     3901 cgccaagctc tatccgccgg gcggcgatgt gatcggacgg cgtcgcgtcg atacccatct
     3961 tgacggcttc cggcagctcg gggcttcggt ccgcgctggc cgcacctatc tctttaaagc
     4021 agcaaagcgc cttgagggac agcgtatcct gctggacgag gcgagtgtga ccgcgaccga
     4081 aaacctgatc atggcggcgg cattggccag ggggacgaca cttctttaca acgcggcctg
     4141 cgagccgcat gtgcaggatc tctgtcgcat gatcaatgcg atgggcggtc gcatcagcgg
     4201 catcggcacg aacctcctga caatcgaggg ggtcgagtcg ttacacggta cgaaacagcg
     4261 ggtggggccg gactatatcg atgcgggcag ctacatggtg gcagcgttgg taaccggcgg
     4321 cgaactgacg gtcgagggtg tcgagccgtc cgatttcgag gcgctggaac ggccgttcaa
     4381 gcgtctcggg gccaagtgga gcttcgaccg cgaggcacgc agcctgcggc tgcccgcgcg
     4441 ccagcggctt aagactgctt atgaccttgg tgacgcgatc cccaagatcg aggacggccc
     4501 gtggcccatg ttcccttcgg acctgatgag tgtgttgatt gtgctggcca cgcagacgcg
     4561 cggcaccacg ctcttttttg aaaagatgtt cgagagccgc atgtatttcg tggaccacct
     4621 gatcggcatg ggcgcgcgca ttgtgcagtg cgatccgcat cgggtggtcg tgaccggtcc
     4681 gacgatcctg cacggcacaa cggtggccag tcccgatatc cgcgcgggca tggcgctgct
     4741 ggtggccgcg ctgtgcgcca aacgtgagac cgtgatctac aacgcgggca gcatcgaccg
     4801 cgggtatgag tcggtggagg tggagctcag ccgtctgggc gcggagatcg aacgagttcc
     4861 gacctgaacc ccgcgtacgg gctttccgcg ccaccggccc tcacgggacg atcacggaaa
     4921 gcgcgtgcgg caggatctcc accgtcacgg gcgtggagcc gatgatatcg ccgtccgcct
     4981 gaaccaccag cggccgcgcg ctgcgcaccg tcaccgcaca ggcggccgcg tgctcatgca
     5041 ggacccggtt cacattgcgc atccgcagca ggcgcatcac atacgaccac gggtattcga
     5101 tcggcctctt catgcagacc acgcaaacgt ccagccgtcc gtcgtccgca cggatgtccg
     5161 gcccgttggg atacaaccct ctcgctagaa tcccgcagtt tgagatcaac acatccgtgg
     5221 catcatacac catagacacc ccgtcgatca cgatttccaa ccggtgcggc cgggcctgca
     5281 gtactttcca caccgcggtc cccacatatg ccgacacccc aaacagagtc ttgccgcgac
     5341 gcgaggtgcg gtcgatcact tcggcgttga cccccacgcc cgcgttcaac agatagatcc
     5401 gcccgttgat ccgcatcacg tccagccgcc gaaaagacga cggacaggct aaccgttccg
     5461 cggcccgttc cacctcaagc ggaataccca attcccgcgc caccagatta ccggtaccca
     5521 tcggcacaat acccagcggc acaccgccgc caacgagcac ctgcgccacc gctgccaccg
     5581 taccgtcccc gccggccgcc accacacact ccaccccccg gtccagaatc gcacggatct
     5641 catccgccag atgatcggca gcaggcaggt ccagcacctc caccgccatt ccgtgcgcat
     5701 cggagacccg cgccaaggcc gcgttcaacc gctcacgcgg ccccgcgcca acggtcggcg
     5761 ccaccagtac gcaggccctc acgcccccac cgcctttgcc gcaaaatgac gccgcgccgc
     5821 atcacaacaa cgcgtcacgc ccgcatctgt gctgagtgtc ttcaaaaaag cctcgcggta
     5881 ctccagcacc cgccgctccg ccgccaaccg caacgatgcg gtataatgca gtccgttgat
     5941 gagccagccg acctggatga gcacaaaatc cgacaggttc cggacggcgt cgtaggagac
     6001 cggctgaccg gcgcagaccg ctgcaacgac atccggcgag ggtgcgccat ccaaccgtaa
     6061 tccccaggcg atcgcaggat tgcgccgcag tgaaccgtcg tcaaccacct cttcgagcac
     6121 acggaaaata tcgagtttgt cggcatcccg aaccagatgc gtcagccgcg ccgcgtcatc
     6181 ggccagtccg tccggcacgg tcttgcggtt gtgcaacgcc accgcagcga gaatccgcgc
     6241 gcgggacgcg gccgcgagac cgtcgagcag gcgttcctcg cgaatgaccg ccaccccgca
     6301 cgccgcatga tcaagggagt ccgcatcccg gaaggtgcca taatcccgca actgcacgtg
     6361 ccggccgatg tcatgcagcc atgcgcacgc ctcgcctgcg gcggtccagg cctcgggcca
     6421 gttctctccg gacatgatcc ggcaagcgtt ctccgccacc cgctcggtgt gttcgatctt
     6481 caaccggtgc agttcggaca acccgccgcg cgcatcgcgg aaactgtcca catacgcccg
     6541 gtaccgccgg atcagggttt tcagaaacgc gcgcgtcata cggtctgccc ggtgaggaaa
     6601 accgctaatg cggtcagcag aaacaggatc atcgttccga tcagaacgcg gtcggtcaac
     6661 aacgtttgct cgggacggcc gccaccgtca tgacggtaga ccagttcgag gtagcggaac
     6721 agaccgaaca ccacaaacgg gatggtgaac cctaaccggt tggtgccgaa gcgccgcacc
     6781 gtctcttccg aaagcgtgta catcgcgtaa cagaccagcg tggcggaagc cgtcaccgcg
     6841 atctgcagat cgagcagata ccggtcatac ccggccaggg ccttacggtg gcgctccccg
     6901 tcctcatcca gcagcacctt ctcgtgacga cgtttgcaga gggccagaaa cagcgcgagc
     6961 agaaaggtgc aaagcagcag ccaaggcgag atgcggaccg acagggccgc ggccccggcg
     7021 atcgcgcgga gcacgaaccc aacggcgatc acgaacacgt ccacatacgg gatgcgcttg
     7081 agtccaaacg tataggccaa ctgcataagt ccgtacgcga gcaacacctc cgtgaacgcg
     7141 cgcggcagag tcagcgcgcc agccgccaaa ccggccgcaa gcaggacgat cgcgaccgcg
     7201 accgcggcgg acgcgcggat ctgtccggcg gcaagcgggc gcagacgttt caacgggtgc
     7261 gcccgatccg cctcgatatc ccgcacgtcg ttgatcagat agatgccgct ggagacacag
     7321 cagaaacaag ccaccgccag agccacgtac acgagaggaa gcactccccg caccccctcg
     7381 cactgagtcg gatcccatcg cgcgaaaaag taggctgcgg gcagcacacc gtttttcagc
     7441 cattgggccg gacgcagagc ccggaaccag atggccgcac gcgttgggat cacgtctctt
     7501 ccctcgggcg cggccgcggc gaccggcgga agccgcagcg cccccacagg cgcagcacca
     7561 tggccaccgc ctcatatgcg atgttggcgc gcatcttgga gtatccggaa cgacggtcct
     7621 caaacgtgat gggcacttcc cgcacggtga agcccaacat ccacgcggta tgggtcatct
     7681 cgatctggaa cgaatagccg ttggaggtga tgcggtcaag atcgatggcc tccagcacgg
     7741 cacgccggaa acacttgaac ccgcccgtgg gatcgttgac cggcataccg gtgatcaatt
     7801 tgacgtactg tgccgccccg gtcgagagca gcaaccggct catcggccag ttcatcacgc
     7861 gaatgccgcc gatatagcgc gacccgagca ccaggtccgc gccttcacgc gcggtttgca
     7921 gcaagacggg cagggctgcg ggatcgtgcg agaagtcgca atccatttcg cagatcagtt
     7981 cgtaaccgcg ctccagcgcc cacttgaacc cggcgatgta ggcgcggcca aggccctcct
     8041 tgcccgcacg atgcatcacg aacacccgcg gctcccgcgc ggcgatcgca tccagcaccg
     8101 cgccggtccc gtcgggcgaa tggtcatcga caaacagtat gtttgccgtg ggcacttcac
     8161 gaaggaccgc ctcggacatg ggttccacgt tctcgcgctc gtcatacgtc ggaatgatga
     8221 tcagtgtttt ttcgctcatt tggcttacag tataccgatt caggggacga gtcttccaca
     8281 caaaaattca gggcaggatc agcatgcagt caccgtaact gaaaaaccgg tagcgttccc
     8341 ggaccgcctc gcggtaggcc tccaggatgc gttcgcggcc cgccaaggcg gcgaccatca
     8401 tgatgagcgt cgatttcggc aggtgaaaat tggtcagcat cgcatcgatg acgcggaacc
     8461 ggtaaggcgg ataaatgaag atcgaactgg agccgaccgc ggccgtcacc tcgccgccgt
     8521 gggcagcggc cacggtctcc agcgcgcgca cactggtgct gccgaccgcc accacacggc
     8581 cgccgcgcgc acggcaggtc gccacagccg cagccgtggc agcaggcacg gcataccgct
     8641 cgggatccat gcggtgctcc tcgacctgct ccgccttgac cggcttgaaa gtgccgggcc
     8701 ccacatgcaa ggtgaccgcg gcgcgcgcca caccccgggc cgcgagggcc gcgaagaggg
     8761 cttccgtaaa gtgcagtccc gcagtcggcg cggccaccgc gccggtttcg cgcgcataga
     8821 tcgtttggta ccgttcacga tccagacggg cctgccgcgc gtccccgcag cgatggatgt
     8881 acggcggtac gggcgtcacc ccgtaacgtt ccaacagcgt catcagcggc accggagaac
     8941 ggaaggcaat ccgccaagcg ttgttcggct ggcgtgccag catctcggct tcaatctggc
     9001 catcggcaaa gaccgcccgc tgccctggcc ggatgggccg tcccgatcca gtgatgcact
     9061 cccaacagac cgtcccctcc gcgcaggccg taccttccgg cgcggggcag ggctccagca
     9121 gcagcaactc caacgcgccg tgcgtgtccg tccagaaacc ttgcagtcgc gccgggaaaa
     9181 cccgtgtgtc gttgagcacg agcaggtcgt ccgcaccgag atatgcgggc agatccatga
     9241 cgtgccggtg ctcgatgcgg ccgtccgaac catgcagcac catcatgcgc gacgtaccgc
     9301 gcacggcggg tggttcctgg gcaatcaggt cgggcggcag atcgtagtca aacgcgcttg
     9361 tcagcatggc gcctgccgct ccctatgcgt tccgtccggg caaggacgtg ttccgggcga
     9421 accgtttggt ctcgggataa tccagcgtgt agtgcaggcc ccggctctct ctgcgtcgga
     9481 cagcactgcg cacgatcaac tcaccgacca gcgccagatt gcgcagttcc agcgtatcgg
     9541 ccgtgaccag ataatcgaag tagtactcgc ggatttcgcg ccgcaggtta cgcagacgcc
     9601 gacgggcgcg ctccaaccgc ttgttcgtgc gcacgatccc gacatagtcc cacatgcagg
     9661 tgcgaacctc catccagttg tgggccacca tcaccgcctc atcactcgcg accgccgtac
     9721 cgtactgcca gtccggaatg cgtatcgcgt ccggcagccg ccccggctgc gccgcaatgc
     9781 ggccagcggt ctcatacgca cagaccaagg cctccagcag cgaattgctg gccaaccggt
     9841 tcgcgccgtg cagaccggtg caggcggtct cgcccacagc gtacaggccc ggcaatgagg
     9901 tcacgccatc cacggaagct cggatgccgc cgcagcagta atgagctgaa ggcacgatgg
     9961 gaatcaggtc atgcgccatg tcgataccga atgaggcgca ggttttgtac agggtcggaa
    10021 aacgcttctg caggaaggcc cggctcttat gccggatgtc gagacagcag tagggcgctc
    10081 cggtgcgttt aatctcggag tcgatcgcgc gggcgaccac atcgcgcggt gccagcgcgc
    10141 cccgcgggtc gtatttctgc atgaaaggac ggccctcggc atccaccagt tcggcaccct
    10201 cgccgcgaac ggcctcgctg atgagaaagc gtttggcttg cggatggtag aggcatgtgg
    10261 gatggaactg gatgaactcc atgttctcga tcgtcgcacc cgcccgccac gccagcgcca
    10321 caccgtcacc cgtggcgatg tcggggttgc aggtgtaaag gtacaccttg ccgcacccgc
    10381 cggtcgccag aatcgtgtcg gccgcgcgga ccgcataaat ctcgccggcg agttgatcga
    10441 gcacatacgc cccgacgcag cggttctcct ccggtcgttt gagccagcgg gtggtgatca
    10501 aatcgatgac catggtgtgt tccaacacct ggatatgggg atgcttcagg atctgctcca
    10561 ccagcaccgc ctcgacgtgc tgtccggtga tgtcccccgc gtgcagcaca cggcgctgcg
    10621 aatggccgcc ctcgcgtccc agatcatagc ccggcacgcc atccgtgcgc gggttgaagc
    10681 acacaccgag tgcctcgaga tccgcgatcc gcgccggccc cgtcgaaacg attttccgca
    10741 ccacctcttc gtcgcagagt ccggctccgg cagtcagcgt gtccgacaca tgctgctcga
    10801 aactgtcccc cgccgccatc acgcatgcga tgccgccctg cgcccggttg gtgttacagt
    10861 catcgatccg ttttttcgtc accagcagca ccctgccgtg cggtgccaga tacagggcgc
    10921 tcatgagacc ggcgataccg ctccccacca caaaaaaatc acaggactcg atgcgcatca
    10981 tcctcccccg cccgccgaac ccggcaggtc atccgttcga aatgccgtga gtatagcata
    11041 cggatggacg tccttccgag cgaaaacggg atcagcatcc cgtcctcgtt ttcgtgtaca
    11101 tcgacgactt gcgacccgcg acgtttaggc tctttggcag ggcgactgcc ccaccgttgc
    11161 ctgcgggaac gcccataagg cactgacgcg ttccgctctt tgaagcgatt ttgcgcttcc
    11221 ctttgtcgga acaacacatt atgctttagc cggaacctat ttttacgggt gattatccaa
    11281 ctgaaaggag caggatatgc gaattctgaa aatggggcag gtcggcggcg ggatcggcgc
    11341 gtttatcggc gaggttcacc ggaaggcggc ccggatggat ggcggaatcg agttgacggc
    11401 gggagccttt gacgtcgttc cgagtaagag tctgcggcag gggaagaacc tcggcctgga
    11461 tcccaagcgc gtgtattcca cctacgagga gatgatcgcc aaggaaagcg cgctgccccc
    11521 gggcgagcgc gtcgatttcg tgtcggtctg caccccgaac cacactcatt tcccgatcgc
    11581 caaagcctgc ctcaaagcgg gtttccacgt catgtgtgaa aagcccatga ccctcacggt
    11641 cgccgaggct gaggagttgg cggcgctcgt caagtccagc ggcaaggtct tcggcctgat
    11701 gcacaactac acggcctacc cgatggtcaa actggcccgt gacatggtga agcagggaga
    11761 cctcggcacg ttgcgcaagg tggtggtcca atacccccag ggttggctgt atcagctcgc
    11821 cgaaaagagc ggcaacatgc aggcctcgtg gcgcaccgac cccaagacct cgggtgtcgc
    11881 cggcgccatg ggcgacatcg gcacgcacgc cgccaacctg gccgagtaca tcaccggcgt
    11941 gaagatcacg cgcgtgctcg cggacctcac cgcctttgtt aaaggacgga aactggatga
    12001 cgacggcaat gtgctgctcc gcttccaaaa gggcgtcaag ggtgtcctgc atgccagcca
    12061 gatctcggtt ggcgaggaga acaatctggc catctgggtc tatggcgata aaaaaggcct
    12121 ctggtggcgg caggagaacc ccaacgaact ccatgtcctc tgccagaccg cgcccgagga
    12181 gatctggcgc cgtggcaacg ggtacgttgc ggagaagagc aaggcggccg cgcgcggcac
    12241 ccgcatcccg agcgggcacc cggaagggtt tctggaggct tttgccaaca attactgcaa
    12301 cttcgcagac accatccggg cgaaggccga aaagcggaaa ccctcacccg aagagttgga
    12361 cttcccgagc gtcgaagccg gcgtccgcgg catgaagttc atccacgcgg tggtgacctc
    12421 ctcgaaaaaa ggcagtgtct gggtcaagat ctaacctgcc tgtttccatc tgtgacacgt
    12481 ggggtgggca taccgatgcc tgcccccgct ttttaccctc cacgcttgaa ggagtctttc
    12541 catgtggtcc ttttcttctg cgttttccct gcccgcaatc ggtctggtgc tgaccctgcc
    12601 gttcgcgacg ctacaagcgc agcaaccggg cgtcacgaac aacatcgcct attacgatga
    12661 cgacgcgccg caaacagacg acgcttaccg gcaatcgcag tgccgtctgg atctgcgcta
    12721 tcccgtatcc aagaccggct tcgccacagt ggtctggttt cacggcggcg gcctcacgtc
    12781 cggacgccgc cacttcattg acctcaaaga tccgtcgatc gctgtagcgg cggtcggata
    12841 ccgtctgtca ccccaagtcg agcaccccgg ttaccttgag gatgccgcgg cagccacggc
    12901 ctgggtgttg cgcaacatcg gacgctttgg cggcgatacc cacaaggtgt ttgtctccgg
    12961 acactccgcg ggcggctatc tcagcgccat gatcggcatg gatccccgtt ggttggcccg
    13021 tcatggactc tcccaccgcc agcttgcggg aatcgtcccg atcagcggac aggtcaccac
    13081 gcactttcat gtgaagaaac tccggggcga cacccagtcg cagtaccgcc ccattattga
    13141 cgagttcgcg ccgctctatt acagttccgc cgagctttcg ccgatctgcc tgattgtcgg
    13201 agaccgccgt atcgaataca aaaaccgtgt ggaggagaac gacctgttgg caaccagcct
    13261 gcggaacttg ggacaccagc atgttgagtt ctatgaaatg ggcggactca atcacagcac
    13321 cgtgggacag ggtgccatga tcctcctgcg tgaattcgtc ggcaagctct cgcgcccttc
    13381 cgacgcagcc aaaacgaaat gacgcgcgcg gcacgcaccc tctaaaaagg acctttgcga
    13441 tgaacccgca ggtcacggga cggattcact ccatcgagtc gttcggcaca ctggacggcc
    13501 ccggcatccg ctatgtgctg tttctgcagg ggtgtccgct ccgctgcctg tactgtcaca
    13561 acccggacgc gggggattgc gaggcgggac gggtcatcac cgctggcgaa gccctgaccg
    13621 acatcctgac ttaccgcgcc ttcatcgcgc gcggcggtgt cacgctctcc ggcggcgaac
    13681 cgctcctcca gcccgaattc gtctgtgcct tgctggaagg gtgccgcgaa cagtgtctgc
    13741 acaccgcgct ggacacgtcc ggcgcaattc cgctctcgat ctcgcgcgcg gccctcgacc
    13801 gtgcggacct gatcctgctg gatgtcaagg cgatcgacga cgacctctgc cgcaccctga
    13861 ccggccgcgg caaccgggaa acgctggcga ccctcgaata ctgcgaagcc gcagggcggg
    13921 aagtctgggt tcgccatgtg ttggttccgg ggtatacgct cgatcccgca cacctcaccc
    13981 gcctggccga ccacctgttg cgctacgcct gcatccgcaa agtcgaattg ctgcccttcc
    14041 acaagatggg cgaatataaa tgggaggcgc tgcagcggcc ttaccggctc agcgacaccc
    14101 ctcccccgac ggaggaggag gttcaggcag cccgcgcgat tttcgagcag cgcgggttgc
    14161 tgtagcggcg tgttgccggg cgcatcgtaa cagccgcgaa agccgttact cccgtgcagg
    14221 aaccgcagct atttgatggt gtagcgggtg gccttgatca gcgccttcca gagcagctcc
    14281 ggcgtctccg ccgcaagcat cgcctcgcga ttttgctctt tcaggaaggt ctcggtcaaa
    14341 cctgacatca ggcgcaggta aaacgcgctg accgccgtgg ggatggtgat gaaaaagacc
    14401 agattcacga tgctgcccgc ttcatcatac cggattccct ttttcgcgac acccaacgca
    14461 agcgtcaagc cccccccctc cacgccgcgc acgtgcggaa aggccagccc gttatccatc
    14521 gccgtactca gaaccgcctc ccgctccaaa gcgctcgcca ccagtttttc cgcgccggaa
    14581 acgaatttct gctgctccat gaccgtcgcc agctccacga tcgcctcacg accgttcgtg
    14641 gccttcaaat ccggaatcat cagcgattca gccgaaaaac gcgaaatgcc cgacttgcgc
    14701 ggctcggtac ggctcggcac gtctccgacc cagcgtggcg cgccgctctc ctcgaacagg
    14761 atgcgtgcgc agctcggaca gttctggatc tgcttgcaga gacgcacgga ctgaacctga
    14821 ctgatcggca accgcatccc gcagatcgcg caacacccgt tgtacatcgg cgccatcacg
    14881 atatggtctt ttttgtacag ccgttgatag agcgatttga tttccggcgg cagcttgtcc
    14941 gtcaactgat cgatcgagtc gttcagacgc tccatgtggg agccgtcccc tgtcgtacgg
    15001 tgctcatccc ggatcaacac gagttcctga agctggttta aatgattgag caggctttgc
    15061 atacgttcct ttcatgcgtt tcaaacggat tccagttcac gcccgcaaga aacgctatac
    15121 cagacaaatt gtgaggatat tgtgaaggaa tgccgtcccg gtgtcaatcc ggga
//
LOCUS       DAOSRB010000051        15138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4023_length_15138_cov_7.766359, whole genome shotgun sequence.
ACCESSION   DAOSRB010000051 DAOSRB010000000
VERSION     DAOSRB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4023_length_15138_cov_7.766359"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLZ74_04360"
     CDS             <1..527
                     /locus_tag="PLZ74_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOR97588.1"
                     /translation="QENHLAVVRNVLDRVLTAGVFDYVLMDCPPSLGILMTSSLAAAD
                     AVVVPMQCEYYAMEGLSVIISLIEQLRGNGVNPGLGLEGILMTMFDGRTRLAADVVAE
                     VRAHFGDKVYETVIPRNVRISEAPSFGQPVVFYDPSSKGAEAYRAFAAEFARRIAGRI
                     EMRAEPAISKAVRV"
     gene            complement(863..2080)
                     /locus_tag="PLZ74_04365"
     CDS             complement(863..2080)
                     /locus_tag="PLZ74_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR97589.1"
                     /translation="MRDIRVLAQKDHISSLCTASPIQALSELVWNALDADAFDVKIDV
                     IQNPLGGIDAIRVSDDGLGINALEADRHFGNLGGSWKRDTARTPLSGRVLHGSKGRGR
                     FKAFSLGNRVEWRTTFQTADGLRSFVLGGAAADPSLFTLVEAATPGPATGTEVMITEV
                     RNAISSLLDTAWAVQQIASNFALYLKAYPNVRIYFQGLLVNPVIVQHTCRTYHLKSSE
                     GNKAELQVIEWKTKQSRGKVVFCNTDGFALHETDAAVRPGSGFNYTAYLVSQRFADLH
                     EANLLLLEEMHPEVRAFLDAARDILRRHFRERREALTAELLSQWQADGVYPYSGAPAN
                     KAERAARARFEACALAIRAYSDNFDILSSTEKRLLFRLLRETLDARPDSALETVADAL
                     GMPPRQRRALLKS"
     gene            complement(2113..3375)
                     /locus_tag="PLZ74_04370"
     CDS             complement(2113..3375)
                     /locus_tag="PLZ74_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR97590.1"
                     /translation="MPRALLSALCCWVAAAFAAFAGPDAADWPQFRGPAGQGIASARN
                     LPRFWSETQNVRWMTRLPGLGHSSPVVAENRVWLTSATDKGRRRHVVCVDLATGAVLR
                     DRVLYTCDEPEPCHNLNSFATPTPVLEQGRVYVTFGKVGTACLSAETGETLWERRDLP
                     VRYFDVGVASSPILHDDRLILTCDGQADDVRYVIALDKHTGRTLWRTDRTFPDGKIPA
                     YTHSSCIPLVIRVAGREQLISPGPYGVRAYDPASGNELWNVRYGGWSVIPRPVFHNGL
                     LVICNGTVKPLMLCIRPEGAAGDATASAVVWQTSRNVPNMPSPLLLGNRLYTLTASRL
                     SCLEADTGAERWSHTVPGQHMASPVAADGHLYLFNTTGIGSVAALGDTFNLVATNRLT
                     EGCMASPAVVGDSLVIRTRTRLYRIGKP"
     gene            3491..3846
                     /gene="ssrA"
                     /locus_tag="PLZ74_04375"
     tmRNA           3491..3846
                     /gene="ssrA"
                     /locus_tag="PLZ74_04375"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3919..4125
                     /gene="rpmE"
                     /locus_tag="PLZ74_04380"
     CDS             3919..4125
                     /gene="rpmE"
                     /locus_tag="PLZ74_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOR97591.1"
                     /translation="MKKDTHPNYDDATITCACGNVIKTRSTRKEMNVNTCSACHPYFT
                     GQQTMLDTEGRVDQFMKRYRIKKA"
     gene            4204..5298
                     /gene="prfA"
                     /locus_tag="PLZ74_04385"
     CDS             4204..5298
                     /gene="prfA"
                     /locus_tag="PLZ74_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOR97592.1"
                     /translation="MLDKAHILQALGRVQAAEAALSDPAVLGNPRVYREKLREHAALK
                     RLESAANAYFKLLDELEGNRALESEPGGDPELAELARSEIARISERVPEAEREVLACL
                     LPSDPRDARNAVFEIRAGTGGEEAALFAAVLFRMYSKFCDAKAWKIAVLDAHATELGG
                     YKEIVFTVSGEGAYGAFKFESGGHRVQRVPETEAQGRIHTSAATVIVFPEADEADEIP
                     LPESELRVDIFCAGGHGGQGVNTTYSAIRITHLPTGLVAQCQDERSQHRNKEKALAVL
                     KARILDQRRQAEEAALGQTRLGLRGSGDRSQRIRTYNFPQNRMTDHRVNLTLYSLDRI
                     VEGELGPLLDALHAYDLDQRLAQESGLRPE"
     gene            complement(5308..5976)
                     /locus_tag="PLZ74_04390"
     CDS             complement(5308..5976)
                     /locus_tag="PLZ74_04390"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOR97593.1"
                     /translation="MTRRFLRGGPCRIARAEADVTETVFVELCAPLAGHLWLQALLVT
                     AGTCFLEDAARCGVSLLVATGHLGWGFAFACMTAGGMSGDIGLYLIGRYATSLLFRLR
                     WVSPSRLEWMEDYFRHHAIKTVVVSRFLPGARTVSNAAAGAVRYPFPRFLLLVFLSTV
                     VQSLLFLQLGVFIGETILPYLRDPRLRIAVFFLVLLMLLAAHRAFVRRRRLAPPDAEG
                     AAGK"
     gene            6262..7713
                     /locus_tag="PLZ74_04395"
     CDS             6262..7713
                     /locus_tag="PLZ74_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97594.1"
                     /translation="MNLTSKRMMMAAACAGLCAFASMTFAGDPTKWWVSDSFEVADGG
                     AADLPISQYKQTVTEIVGGGGATTTNFLWVADGLDASKIIATNSYIGQPFITNSGLRE
                     LVLELATEGSTLTRHVIGDNGVSTGKAFSASAPVYVDTMIQFRPSEDTPTIGDDVKIA
                     VYVNVNSNLVVYHGMDGADPTNSVFETEFINPDTWYRLTIEMTGYDDWTPACKVYLNK
                     IQLSHANALEGGLFRTAGPGGTLNAVAFQGTGLVDDLAVADDSDAVSPAAVMLTVNIV
                     GSGSVLTNGAAVANGGEVQSGTAIVPTPDDWHRLHGVTGTEASYDGDTGIVTANPNAN
                     DPSVTVTFTNVTGTLAMGGKDVSAEKLAAWATGKNTPALTEAQVSANGAAWYDEYLLN
                     IAKNDLASTIEITSISVDAAEATIELAASSTAVDFTASALNGTLNVYTTDNLGTAFTL
                     VQTEYVTVTPGADGTATVKVPVAQGNFIKAVVE"
     gene            8102..9577
                     /locus_tag="PLZ74_04400"
     CDS             8102..9577
                     /locus_tag="PLZ74_04400"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOR97595.1"
                     /translation="MKRSKRRFGVVALLVLALVLSFPHGAARAAGVLDFRQGFSGVAS
                     KATAAVVFIQVEKQVPAGNMQYYFNNPYDLFGEEFFERFFGRRQPRQQMPRRRQPQQM
                     FKQTGQGSGFLISKDGYILTNTHVVGDVDKITVRLSDGREFAAKRIGADVKTEVALIK
                     IEAKEDLPYLEIGKPEELKIGEWVIAIGNPFGLKETLTVGVVSAKGRSNIGITDYEDF
                     IQTDAAINPGNSGGPLLNIDGKVVGINTAIYSRSGGYMGIGFAVPIDMAVRIKDQLIA
                     TGKVTRGYVGVFLNPGEVTREMAASFGRSEAGGVLIAEVQKDGPADKAGIKSGDILIE
                     LNGKKIVGNTDFRNDVARIMPNEKAEVLLFRDGKTRKVTVTVAAFPEDEQGTDAGGES
                     GGAALLEKLGLQVQDLTQEIAQQLGYENLRGVVISDVMQGSVADEAGLRPGMLILEVN
                     RAEVKNARQFEAAVGKAKGSNVLVRVKTKEGTIFLNLPTQE"
     gene            complement(9715..10647)
                     /locus_tag="PLZ74_04405"
     CDS             complement(9715..10647)
                     /locus_tag="PLZ74_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOR97596.1"
                     /translation="MPTPVLHSVLPVCLLVLLGAALRHLRFLPDLFFVQLNRLGFWLL
                     LPSLLFSTLANAPSQTSSEGFRISMLLCVSAAGMILAGVVVSCLLRLPEPSARALIQA
                     AMRGNLAYTALPILLYAYGENSAAAVTATLAWAPTIPFYNFCAVLILTPKGAGSPHSR
                     FLRVASDILRNPLILGCGAGFIVLWSGLRPPTACMRAIESLGRAALPCALLSLGAGLN
                     FSALRTHGSAALAATALKLLASPALGRALAPAFGVHGDARMIALLYLATPCAVTSYVM
                     ADQMGADRELAASTIALSTLLCIPVMIFLMLCLR"
     gene            complement(10700..11566)
                     /locus_tag="PLZ74_04410"
     CDS             complement(10700..11566)
                     /locus_tag="PLZ74_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOR97597.1"
                     /translation="MPDPHISSFANPRVKHAVRLRQRSFRDETGQMLIEGYRECKRAL
                     DHGYRPQILFCCEELYLKNLNEPEIVRRCREAGAEILSCSAPVFGKMAYRERPEGLLM
                     IGPHLTRRLEDLRLPDPALIVVAEAVEKPGNLGTILRSADAAGVQAVIVCDRCTDIHN
                     PNVVRASTGTLFSLPVIEADSGDTLAFLHARGFRILATTPHAEPLHTEVPLTGNVAIA
                     VGSEQYGLTEKWMGAADLRVRIPMLGIADSLNVSAAATILLFEALRQRIAAGQARVPP
                     HEAWHGEAMFDA"
     gene            complement(11578..14664)
                     /locus_tag="PLZ74_04415"
     CDS             complement(11578..14664)
                     /locus_tag="PLZ74_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucokinase"
                     /protein_id="HOR97598.1"
                     /translation="MSFDLLVLTAAHEAQAAGYRAQLAWRRAVGLLAPETRTLVVADP
                     GGRRVGSLGATLHVLALLAEEAGRVKSVADAFAGRRILICHSGGDSRRTPAYAAQGKV
                     FTPVPTCDDHGTPLALFDLILRNVSALPAPAAGQVLITSGDVLLTFDASAVDLSRPGI
                     TGIAAFGPTERGSRHGVYVPASFCPNPQRAECVPVLDFLQKPTEAEAEARGAVDPFGH
                     VAVDTGLLSLDPAGCARLLAAAGWSRKETGHGKSKTRHVTKGLLAEILAGHSPALDLY
                     EELTMAVSPRYDEARYLTRLVTERGRDAAHSRRMRVFYRAVRPLAFHVNLVPYCDFFH
                     IGSSRELLVGFSELSRTARTYRFENGCGAKIRGGVSERAFLFNARIDCPLQTGRALLE
                     AVHAPSTCPGLFLDGDNLVTGLPPDATVAIRLPPRTGLVCLPVGRSRWAAVVYGLEDD
                     FKTPFGGAKPCLFLNTPMDTWMARTGLTPRRLWRGEREKRDGLWQARLWRIGALNDVL
                     SEAVALAASGTAPQPAPALTKAGTRFSLAELLPRVNHTRLLGGRQELRRQVCLLRITE
                     YLLAHDTLPSAELCGWIRTADEARQTEKALSDLLSNEARDNPLLRARILRLLTLIRER
                     WAGAEAAQESRTRTFAAVAEAVAFHFKLPAAPRPAAILHDQVVWATTPVRIDFAGGWS
                     DTPPICTEQGGTVLNAAVTLNGLYPIQVMAKLNTAGRIRLASIDLGERREFRSAAELL
                     DHHDPTDWAALAKAALVLTGIAPPDARASLSRWLDTLGGGLDLTLFSALPKGSGMGTS
                     SILGAAIIACLEHVLGLPLQSDRLIRMTSILEQRLCTGGGWQDQVGGILPGVKLIRTQ
                     PGPDQTVSLRWTPFDMSEGSELRRRCLLYYTGQKRMARNILHNVVSGYLARDPALLRT
                     VGEIKNLALEVKEALDAQDLDAFAHGLRRYWELKKRIDPGSTNPQIESILNRVARDTQ
                     AALLPGAGGGGFILLLANSAAAAARIRRTLEAAPPNGQARFFDFAVDTQGLKITVL"
BASE COUNT         3028 a         4343 c         5143 g         2624 t
ORIGIN      
        1 ggcaggagaa ccacctcgcc gttgtgcgga acgtgctcga ccgcgtgctc accgcgggcg
       61 tgttcgacta cgtgctcatg gactgccctc cctccctcgg cattctcatg acctcgtcgc
      121 tcgcagcggc cgatgccgtc gtggtcccca tgcagtgcga atactacgcc atggaagggc
      181 tgagcgtcat catctccctg atcgagcagt tgcgcggcaa cggggtgaac ccggggctgg
      241 ggctggaggg gatcctgatg accatgttcg acggccgcac gcggctggcg gcggatgtgg
      301 tggcagaggt gcgggcgcat ttcggcgaca aggtgtatga aaccgtgatc ccgcgcaacg
      361 tgcgcatcag cgaggccccg agcttcggtc agccggtggt cttctacgat ccctccagca
      421 agggcgcgga ggcgtaccgc gcctttgccg cggagttcgc gcgccgcatt gcggggcgta
      481 ttgagatgcg ggcggagccc gcaatctcaa aggctgtgag ggtgtgaggg tgttaggctg
      541 tgagggtgtt aggctgtgag gctgttagac tgtgagggtg tgagggtgtt agggtgtgag
      601 gcaatcgatc ccgatagcga taccgatagc gatcccgatt tggatgaaac cttgaaacca
      661 gaaacatcga tagcgattcc gatagcgata ccgataccga tttggatgaa accctgaaac
      721 catgaaaccg tgaaaccatg aaaccgtgaa accagaaacc cagaaacctg aaacatcgat
      781 agcgatcccg actaataacg cactaacgca ctaatgcact aatgcactaa tgaactaatg
      841 aactaacgca cggttttcag gttcacgatt tgagcagggc gcggcgttgg cggggcggca
      901 tcccgagcgc gtcggccacg gtttccagtg cggagtcggg gcgggcgtcg agggtttcgc
      961 gcagcagacg gaagagcagg cgtttctctg tggaggagag gatgtcgaaa ttgtccgagt
     1021 aggcgcggat cgcgagcgcg caggcctcga aacgcgcgcg ggccgcgcgt tcggctttgt
     1081 tggccggcgc gccggagtag ggatagacgc cgtccgcctg ccattggctc agcagttcgg
     1141 cggtcagggc ttcgcggcgt tcgcggaaat gccgacgcag gatgtcgcgc gccgcgtcca
     1201 gaaaagcgcg cacctccggg tgcatctctt cgaggagcag caggttcgcc tcatgcaggt
     1261 cggcgaagcg ttgcgagacg aggtaggcgg tgtagttgaa tccgctgccg ggccgcaccg
     1321 ccgcgtcggt ctcgtgcagc gcgaagccgt cggtgttgca gaagaccacc ttgccgcggc
     1381 tctgcttggt tttccactcg atcacttgca gttcggcctt gttgccttcc gaggacttca
     1441 ggtggtaggt gcggcaggtg tgctggacga tgaccggatt gaccagcagg ccttggaaat
     1501 agatgcggac attggggtag gccttgagat agagcgcgaa gttcgaggcg atctgctgga
     1561 cggcccaggc ggtatcgagc agcgagctga tcgcgttgcg gacctcggtg atcatgacct
     1621 ccgtgccggt cgccgggccc ggcgttgcgg cttcgacaag cgtgaacagg ctggggtctg
     1681 ccgcggcgcc gcccaacacg aacgagcgca ggccgtcggc ggtctggaag gtcgtgcgcc
     1741 attcgacgcg gttgccgagg ctgaaggcct taaagcggcc cctccccttg ctgccgtgca
     1801 gcacgcgccc ggagagcggc gtgcgcgcgg tgtcgcgttt ccacgagccg ccgaggtttc
     1861 cgaagtgccg atccgcctcc agggcgttga tgccgaggcc gtcatccgac acgcggatgg
     1921 cgtcgatccc gccgaggggg ttctggatca catcgatctt cacatcgaac gcgtccgcat
     1981 ccagcgcgtt ccagacgagt tcgctcagcg cctggatggg cgaggcggtg cacagcgagg
     2041 agatgtggtc cttttgggcc agaacgcgaa tgtctctcac aacaggatcc ctttcttttc
     2101 cggtcgcgcg cgttacggtt tgccgatgcg gtaaagccgg gtacgcgtgc ggatgaccaa
     2161 tgaatcgccg accacggcgg gcgaggccat gcacccttcg gtcaggcggt tggtggccac
     2221 cagattgaac gtgtcgccaa gcgcggccac cgaaccgatg ccggtggtgt tgaaaaggta
     2281 aagatggccg tccgcggcaa cgggcgaggc catgtgctgt ccgggtacgg tgtgggacca
     2341 gcgctccgcg ccggtgtcgg cctcaaggca gctcagccgc gaggcggtga gggtatagag
     2401 gcggttgccg aggagcaggg gcgaaggcat gttcggcacg ttgcgggagg tctgccagac
     2461 caccgccgag gcggtggcgt cgcctgcggc cccttcggga cggatgcaga gcatcagcgg
     2521 cttgaccgtg ccgttgcaga tgacgagcag gccgttgtgg aagaccggcc gggggatgac
     2581 cgaccagccg ccgtagcgca cgttccagag ctcgttgccg gacgcggggt catacgcgcg
     2641 cacgccgtag gggccggggc tgatgagctg ttcccgtccg gccacgcgga tcaccagcgg
     2701 tatgcaggac gagtgcgtgt aggccgggat ctttccgtcg gggaaggtgc ggtccgtgcg
     2761 ccagagcgtc cggccggtgt gtttgtcgag cgcgatcacg taacgcacgt catcggcctg
     2821 cccgtcgcag gtgaggatca ggcggtcgtc gtgaaggatc ggcgagctgg cgacgccgac
     2881 atcgaaatag cgcaccggca ggtcgcgccg ctcccagagg gtctccccgg tctcggccga
     2941 gaggcaggcg gtgccgactt ttccgaaggt gacatagacg cggccctgct ccaaaaccgg
     3001 ggtgggggtt gcgaagctgt tgaggttgtg gcagggctcc ggttcgtcgc aggtgtacag
     3061 cacgcggtcg cgcagcacgg cgccggtggc gaggtcgacg cacaccacat ggcgccgccg
     3121 ccctttgtcg gtggcgctgg tcagccagac gcggttctcc gcgaccaccg gcgacgagtg
     3181 gccgaggccc ggcaggcggg tcatccagcg cacattttgc gtttcgctcc agaagcgcgg
     3241 caggttgcgg gcagaggcga ttccctgtcc ggcgggtccg cggaactggg gccagtcagc
     3301 cgcgtccggt ccggcaaagg ctgcgaaggc cgccgcgacc cagcagcaga gtgcggaaag
     3361 aagggcgcgc ggcatctggc aaggcgtaca gcacatacgc gaactatacg aaaaaaggag
     3421 gggtgctgtc cacgcgcgaa aatccgggtt tactttttgc gaatctgtgc gatactggtc
     3481 tgcgttttga gggggcgact ggtttcgacg gggcacgtga aaatccggtt gcgcgccgag
     3541 ggctcctgtt ggcctcgtaa aaaaacagga aaaaacctaa acgcgaacga taacttcgct
     3601 ctcgcggcct aattattagg cccgacgtgg aaccgccgat gtctgctgag cggggaagcg
     3661 acgacgagca gaccctgccg ggggccgagt agccgggcgc ccgtgtcttg acgaatcgtg
     3721 gttgctggtc ttcgggtaag cctgtccgcc ggccttcccg atgatgagac tgaaagacgg
     3781 gacacgcgcg tagagacggg atggaagctg tttcggacgg gagttcgatt ctccccgcct
     3841 ccaccatttt tcgcgcttgc gccgtgacgg gggtttgcgc taaccttatc catccgtgtt
     3901 acggaaaggc gtttttacat gaagaaggat acccacccca actatgatga cgcgacgatc
     3961 acgtgtgcct gcggcaatgt gatcaagacg cgttccaccc gcaaagagat gaacgtcaac
     4021 acctgctcgg cctgtcatcc gtatttcacc gggcagcaga ccatgctcga caccgagggt
     4081 cgcgttgacc agttcatgaa gcggtacaga atcaagaagg cttagtgctt cggggtcggg
     4141 gcgggccggg ggcgttgccg cggggcggca gtcgcagttc ctggcccgcc ttgtcttttg
     4201 cctatgctgg acaaagccca catccttcag gctctcggcc gggttcaggc tgcggaagcg
     4261 gccctctccg atcccgctgt tctggggaat cccagggtgt accgggaaaa gcttcgtgag
     4321 catgcggccc tgaagcgttt ggagagcgcg gccaacgcct atttcaagct gttggacgag
     4381 ctggagggca accgtgcgct ggagtccgag ccggggggag atccggagtt ggccgaactg
     4441 gcgcggtcgg aaatcgcgcg gatcagcgaa cgggtgcccg aagccgagcg cgaagtgctg
     4501 gcctgtctgc tgccgtccga tccgcgcgac gcccgcaacg cggttttcga gatccgcgcg
     4561 ggaaccggcg gtgaggaggc ggcgctgttc gctgcggtgc tgttccgcat gtattccaaa
     4621 ttctgcgacg cgaaggcgtg gaagatcgcg gttctggacg cgcacgccac ggagttgggc
     4681 ggctacaaag agatcgtgtt cacggtgagc ggcgaggggg cctacggcgc gttcaagttc
     4741 gagagcgggg gccaccgggt gcagcgcgtg ccggagaccg aggcgcaggg ccgcatccac
     4801 acctccgccg ccacggtcat tgtcttcccc gaagcggacg aggctgacga gattccgctt
     4861 ccggagagcg agttgagggt ggacatcttc tgcgcgggcg ggcatggcgg tcagggcgtg
     4921 aacacaacct attcggcgat tcgcatcacc cacctgccga ccggactggt cgcgcagtgt
     4981 caggacgagc ggtcgcagca ccgcaacaaa gaaaaggcgc tcgcggtgct caaggcgcgg
     5041 attctggatc agcggcgtca ggccgaggag gccgcgctcg ggcagacccg gttgggtttg
     5101 cggggatcgg gggaccgcag ccagcgcatc cggacctaca acttcccgca gaaccggatg
     5161 accgatcacc gcgtcaatct gacgctctac agtctggacc ggatcgtgga gggagaactc
     5221 gggcccctgt tggacgcact gcatgcgtat gatctggatc agcgtctggc ccaagaatcc
     5281 gggctccggc cagagtagcg gagcggccta ttttccggcc gcgccttccg catccggcgg
     5341 cgcgagcctg cgccgtcgca caaacgcgcg gtgggccgcc aacaacatca gcagtaccag
     5401 aaaaaagacc gcgatacgca gacggggatc acgcaggtag ggcaggatcg tctcgccgat
     5461 gaacaccccc agttgcagaa acagaaggga ctgcaccacg gtggagagaa aaaccagcag
     5521 gagaaaacgc gggaacggat accgcacggc tccggccgcg gcattgctca cggtgcgggc
     5581 tcccggtaga aaacgcgaga ccactacggt tttgatggcg tggtggcgga agtaatcctc
     5641 catccactcc agacgcgacg gggagaccca gcgcaggcgg aacaggagcg aggtcgcgta
     5701 cctcccgatc agatagagac cgatgtcacc ggacatcccg cccgccgtca tgcaggcgaa
     5761 agcaaacccc catcccaagt gacccgtagc caccagaagg ctcacgccgc atcgggcggc
     5821 atcctcgaga aagcaggtcc cggccgtcac cagcaaggct tggagccaga ggtggccggc
     5881 aaggggggcg cataattcga cgaatacggt ttcggtcaca tcagcctcgg cgcgcgcaat
     5941 ccgacacggg cctccccgga ggaatcggcg cgtcacgttt ccccggcccc tgtgccggac
     6001 gggacgggca ttccggagtt ttcaggaaaa cccggggcca gtatgacaca tcccgcttcg
     6061 ggctggcaag gaggggcaga aaaaaaaacg caaaaaagtg gttttcgggc ttgctttgcg
     6121 aggccttttc ggcgtatcct ttgactacaa cgaaagaatg gtcttgtctg gaaacagaag
     6181 aacgggtgtt tttctgtggc cggataggat tttgaaggtt tagagtcaat aacgaaggaa
     6241 cacgatagga gggtttctta aatgaacctg acttcaaagc ggatgatgat ggcggcggca
     6301 tgcgcggggc tttgcgcctt tgcgtctatg acgttcgcgg gcgacccgac aaaatggtgg
     6361 gtctccgatt cgttcgaggt cgccgatggc ggggccgcgg atctcccgat ctcacagtac
     6421 aagcagacgg ttacggaaat cgtgggaggc gggggtgcaa cgacaaccaa cttcctctgg
     6481 gttgcggatg gattggacgc atccaagatc atcgcgacca actcctacat tgggcagcct
     6541 tttatcacca actctgggct cagggagttg gttctggaac tcgcgaccga gggcagcacc
     6601 ctcacgcggc acgtgatcgg ggataacggt gtttcgacgg gcaaggcgtt ctctgcatca
     6661 gcgccggtct atgtcgacac gatgatccag ttcaggccga gcgaggacac gcccacgatc
     6721 ggagatgacg tcaagattgc ggtctacgtg aacgtcaact cgaatctggt ggtttaccat
     6781 gggatggatg gggctgatcc gaccaacagt gtgtttgaga cagaattcat caatcctgat
     6841 acatggtacc gtctgactat tgaaatgacg gggtatgatg actggacccc ggcgtgtaag
     6901 gtttatctta ataagataca actgtctcat gcgaacgcgc ttgagggtgg cctcttccgg
     6961 actgcgggtc ccggcggcac gctcaacgcc gtcgctttcc agggaaccgg tctggtggat
     7021 gatctggcgg tggcggacga ctccgacgcg gtctctcctg ctgcggttat gctgacggtg
     7081 aatatcgtgg gttcgggatc cgtcttgacc aacggtgcgg cggtcgccaa cggcggcgag
     7141 gtgcagagcg gaacggccat cgtgccaacc cccgatgact ggcatcggct ccatggcgtc
     7201 acgggtactg aagcgagcta tgacggcgat accggcatag tgaccgccaa cccgaatgcg
     7261 aatgacccga gtgtcacggt gaccttcacg aatgtcaccg gcaccctcgc catgggcgga
     7321 aaagatgtta gcgccgagaa gctggcggca tgggccactg gaaagaatac tccggcgctc
     7381 accgaagcgc aggtttcggc gaatggcgca gcgtggtacg acgagtacct gctgaacatt
     7441 gcaaagaatg atttggccag caccatcgag atcacctcaa tctccgtgga tgcagcagaa
     7501 gcgaccattg aattggcagc ctcttcgact gcggtcgact tcactgccag cgctctcaac
     7561 ggaacgctga acgtctacac cacggacaat ctgggaacgg ccttcaccct ggttcagacg
     7621 gaatacgtca ccgtcacccc cggagcggat ggaacagcga ctgtcaaggt tcctgtggct
     7681 caggggaact tcatcaaggc agtggttgaa taaccggttc tgaccgagag ttgatacaag
     7741 acatgcaggg gtccgcggaa acgcgggccc ctgttgtttt atgcgacaac ctgacgcggt
     7801 gaaaaggaca aggtgtcccg tgtgaaaagc aacgatgatt cataaatgat agaaaaggct
     7861 aatttaaggc gtaaaatgtt atatattcac tcgaatggat cgaaaaacaa gttatattga
     7921 atatggcatg gttggtgctt taagcaaatc ggcttagcaa gaggcggcaa gaggtaacac
     7981 aaaacaggtc cgtagaggcc ataatctcat acgcttgcga agtcgggatg gaaaaagagc
     8041 ggcttgccgc caccgcatga ggttatggcc ttttccatcc gcgggatgag gaggaaagag
     8101 tatgaaacgg agtaaacgta ggttcggcgt cgtggctttg ctggttctgg ccctggtttt
     8161 gtctttcccg cacggggcgg cgagggcggc aggggtgttg gatttcaggc aggggttttc
     8221 aggtgtcgca tccaaggcca cggcggcggt ggtctttatc caggtggaga aacaggttcc
     8281 ggccggcaat atgcagtatt actttaacaa cccctatgac cttttcgggg aggagttctt
     8341 cgagaggttc ttcgggcgac ggcagccccg gcagcagatg ccgcggcggc gccagccgca
     8401 acagatgttc aagcagaccg gacagggttc cgggttcctg atcagcaagg acggctatat
     8461 cctgaccaac acccacgttg tcggagatgt cgataagatc acggtgcgcc tgtctgacgg
     8521 ccgggaattc gccgcaaaac ggattggcgc cgacgtgaaa accgaggtcg cgctgatcaa
     8581 aatcgaggcg aaggaggatc tgccctatct ggagatcggc aagccggaag agttgaaaat
     8641 cggcgaatgg gtgatcgcca tcggcaaccc gttcggactg aaggagacat tgaccgtggg
     8701 ggtggtgagt gctaaaggac gcagtaacat cgggattacg gattacgagg acttcattca
     8761 gacggacgcc gcgatcaatc cgggaaactc gggcggcccc ctgctgaata tcgacggcaa
     8821 ggtcgtcggg atcaacaccg caatctacag ccgcagcggc ggatacatgg ggatcggttt
     8881 cgcggtgccc atcgacatgg cggtcagaat caaggaccaa ctcatcgcaa ctggcaaagt
     8941 gacaaggggt tatgtcggcg tgttcctcaa tcccggcgag gtcacccggg agatggcggc
     9001 atccttcggg agatcggaag cgggcggagt cctgatcgcc gaggttcaga aggacggtcc
     9061 tgcggataag gcggggatca agagcgggga tatcctgatc gaactgaacg gcaagaaaat
     9121 tgtggggaac accgacttca gaaacgatgt ggcacgaatc atgcctaatg aaaaagcgga
     9181 agtgctgctg ttccgtgacg gcaagacgcg taaggtcacg gtgacggttg cggctttccc
     9241 cgaggatgag cagggaacgg atgccggagg cgaatccggt ggggcggccc tgctggagaa
     9301 actcggactg caggttcagg atctgacgca agagatcgcg cagcagctgg gttacgaaaa
     9361 tctgcggggc gtcgtgattt cggatgtgat gcagggctcg gttgcggatg aggcggggtt
     9421 gaggcccgga atgctgattc tggaggtcaa ccgggcggag gtgaagaacg cgagacaatt
     9481 tgaggcggcg gtcggaaaag cgaaaggaag taacgtgctg gtgcgcgtga agaccaagga
     9541 gggcacgatc ttcctgaatt tgccgaccca agagtaacga tcaaccacac aacccaagta
     9601 tatatagacc actcctgtca tgcagcaccg gggccgggta aaaccggccc cggttttttt
     9661 gcgaacaaat gccccggtta tagggttgcg ggctttaccc gcgtggaacc cgcgtcagcg
     9721 gagacaaagc atcaggaaaa tcatcaccgg tatgcagagc agcgtgctca acgcgatggt
     9781 ggaagccgcc agttcgcggt cagcccccat ctggtcagcc atgacgtagg aggttacggc
     9841 acaaggggtg gccagataca ggagcgcgat catccgcgca tcgccatgca cgccgaacgc
     9901 gggcgccaag gcccggccga gggcgggaga ggccagcaat ttgagtgcgg tcgccgcgag
     9961 tgcggcgcta ccgtgggtac gcagcgcgga gaagttcagg cccgcaccca gagagagcag
    10021 tgcgcagggc aacgcggcgc ggccgagaga ttcgatggcc cgcatgcagg cggtcggcgg
    10081 gcggagaccg ctccacagca cgataaagcc cgcgccgcag ccgaggatca gcgggttgcg
    10141 caggatgtcg gaggcgacgc gcaagaaacg ggagtgcggc gagcccgcgc ctttgggggt
    10201 gagaatcagc acggcacaga aattgtaaaa cgggatggtc ggcgcccagg ccagggttgc
    10261 ggttaccgca gccgcactgt tttcaccata cgcgtagagc agaatgggta gcgcggtata
    10321 ggcgaggttg ccccgcatgg cggcctggat cagggcgcgg gcagagggtt ccggcaggcg
    10381 cagcagacac gacaccacca cgccggccag aatcatgccc gccgcgctga cgcagagcag
    10441 catggagatg cggaagcctt cgctgctggt ttgcgaaggc gcgttcgcga gggtgctgaa
    10501 caacagtgaa ggcaagagca gccagaaccc cagacggttc agctgcacga aaaaaagatc
    10561 gggcagaaac cgcagatggc gaagcgccgc cccgagcagg accagcaggc agacgggcaa
    10621 cacggaatgg aggaccgggg taggcatatt tcgcgcacag tagtatagcg gcctgtcacg
    10681 cggcggcgca aggcaggatt caggcatcga acatcgcttc gccatgccat gcctcgtgcg
    10741 gcggcacgcg cgcctgtcct gcggcgattc gttgccgcaa cgcttcgaac agaagaatgg
    10801 tggcggcggc ggaaacgttc agcgaatcgg cgatgcccag catcgggatg cgcacgcgca
    10861 gatcggccgc gcccatccat ttttcggtca gcccgtactg ttccgacccc acggcaatcg
    10921 ccacgttgcc ggtcagcggg acctcggtat gcaggggttc cgcgtggggc gtggttgcca
    10981 gaatgcggaa cccgcgggcg tgcaggaaag ccagcgtgtc cccgctgtcg gcttcgatca
    11041 ccggcaggga gaaaagcgta ccggtcgagg cgcgcaccac gttggggtta tggatatcgg
    11101 tgcaacggtc gcacacgatc accgcctgca cacctgcggc atcggcggaa cgcaggatgg
    11161 tgcccagatt gccgggtttc tcaacggctt ccgccaccac gatcagcgcg gggtcgggaa
    11221 gtcggagatc ctcgagtcgg cgggtcaagt gggggccgat catcaaaaga ccttcgggac
    11281 gttcgcggta ggccatcttc ccgaagaccg gtgcggagca ggaaaggatc tcagccccgg
    11341 cttcccggca gcggcggacg atttcaggct cgttgagatt tttcaggtaa agttcctcgc
    11401 agcaaaagag gatctggggc cggtacccgt gatccagggc gcgtttgcat tcgcggtagc
    11461 cttcaatcag catctggccg gtttcatcgc ggaacgagcg ctggcggagc cggaccgcat
    11521 gcttgacgcg cggattcgcg aacgaggaga tatgcggatc aggcatgggg ttccctgtta
    11581 caataccgtg atcttgagcc cctgggtgtc cacggcgaag tcgaagaaac gcgcctgccc
    11641 gttcggcggg gcggcttcca acgtgcggcg gatgcgcgcc gcggccgcgg cactgttggc
    11701 gagcaggagg atgaacccgc cgccgcccgc gccgggcaac agcgcggcct gcgtgtcccg
    11761 ggccacgcgg ttcagaatcg attcgatctg cggattggtc gagcccgggt cgatgcgttt
    11821 cttcagttcc cagtagcgcc gcaacccgtg cgcgaacgca tcgagatcct gggcgtcgag
    11881 ggcctctttg acctccagcg cgaggttttt gatctcgccc acggtccgca acagcgccgg
    11941 atcgcgcgcg agataaccgg acaccacgtt gtgcagaatg ttacgcgcca tgcgtttctg
    12001 gccggtgtaa taaagcaggc agcggcgtct gagttcactg ccttccgaca tatcgaaggg
    12061 ggtccagcgt aaggacacgg tctgatccgg tccgggttgc gtacggatca gtttcacgcc
    12121 gggcaggatt ccgccgacct ggtcctgcca gccgccaccg gtgcacaggc gctgttccag
    12181 aatcgaggtc atccggatca ggcggtcgga ttgcaacggc agtcccagca catgttcgag
    12241 acaggcgata atcgcagccc cgagaatcga gctcgtgccc atgcccgacc ctttgggcag
    12301 ggcggagaag agggtcaggt ccagtccccc gcccagcgtg tcgagccacc gggacagcga
    12361 cgcccgcgcg tcaggcgggg cgatgccggt caggacgagg gcagctttgg ccagtgccgc
    12421 ccaatcggtc ggatcgtgat gatcgagcag ttcggcggcg ctgcggaact cccgccgctc
    12481 gcccagatcg atactggcga gccggatccg gcccgcggtg ttcagtttgg ccatgacctg
    12541 aatcgggtag aggccgttga gcgtgacagc ggcattgagc accgtgccgc cctgttccgt
    12601 gcagatcggc ggcgtatcgg accagccgcc cgcgaaatcg atacgcaccg gtgtggtcgc
    12661 ccagaccacc tgatcgtgga gaatcgcggc gggacgcggt gcggcgggaa gcttgaaatg
    12721 gaacgccacc gcctcggcca ctgcggcaaa ggtgcgggtc cgggattcct gcgccgcttc
    12781 cgctccggcc cagcgttccc ggatcagggt gagcagacgc agaatgcgcg cgcgcagaag
    12841 cgggttgtcg cgggcttcgt ttgagagcag atcggacagt gccttttcag tttgccgggc
    12901 ctcatccgca gtgcggatcc atccgcacag ttccgcagac ggcagcgtgt catgggccag
    12961 caggtattcg gtgatgcgca ggaggcacac ctgccgccgc aattcctgcc gcccccccag
    13021 cagacgggtg tggttcacgc gcggcagcaa ctccgcgagg ctgaagcgcg ttccggcttt
    13081 tgtgagcgcg ggagcgggtt gcggtgcggt gccggatgcg gcgagggcca cggcttcgga
    13141 caagacatcg ttcaacgcgc cgatacgcca caaccgcgcc tgccagagcc cgtcgcgttt
    13201 ttcgcgctcc ccgcgccata gccgccgcgg cgtcaggccg gtgcgcgcca tccacgtatc
    13261 catgggcgtg ttgaggaaca ggcagggctt cgcgccgccg aacggggttt tgaaatcgtc
    13321 ctcaagtccg tagaccacgg cggcccaccg gctacgtccg accggcaggc agacgagacc
    13381 tgttcgcggc ggcagccgga tggccacggt tgcgtcgggc ggcagtccgg tcacaagatt
    13441 gtcgccatcg aggaaaaggc cggggcatgt gctcggggcg tggaccgctt cgagcagcgc
    13501 gcggccggtc tgaagggggc agtcgatgcg cgcattgaac aggaaggcgc gttcggacac
    13561 tccgccgcgg attttggcgc cgcagccgtt ttcaaaccgg tatgtccggg cggtacgcga
    13621 gagttccgag aagcccacga gcaactcccg gctgctgccg atgtggaaaa aatcgcagta
    13681 gggaacgaga ttcacgtgga acgccagcgg ccgtacggct cggtaaaaga cgcgcatacg
    13741 gcggctgtgg gccgcatccc ggccgcgttc cgtcaccagg cgggtgagat aacgcgcttc
    13801 atcgtaacgc ggcgagaccg ccatggtcag ctcttcatac agatcaagcg ccggactgtg
    13861 tcccgcgagg atctccgcca gcaggccttt cgtcacgtga cgcgtcttgg actttccgtg
    13921 tcccgtttct ttcctgctcc atcctgcggc ggccagcagc cgcgcgcagc ctgcggggtc
    13981 aaggctcagc agaccggtat ccactgccac atgcccgaag gggtccaccg cgccacgggc
    14041 ttccgcctcc gcttctgtgg gtttctgcag gaaatcgagg accggcacgc attcggcgcg
    14101 ctgcgggttg ggacagaagg aggccggcac gtacacgccg tgacgcgaac cgcgttctgt
    14161 cgggccgaac gcggcgatgc ctgtgatgcc cggccgcgag agatccacgg ccgaggcgtc
    14221 gaaagtcagc agcacatcgc ctgaggtgat cagcacctgg cccgccgcgg gggcgggcag
    14281 ggccgagacg ttgcgcagga tcagatcaaa gagcgccagc ggcgtaccgt gatcgtcaca
    14341 cgtcggaacc ggcgtgaaga ccttgccttg cgccgcatag gccggagtgc gtcggctgtc
    14401 gccccccgag tggcagatca gaatgcgtcg tccggcaaag gcgtcggcaa ccgatttgac
    14461 gcgccccgcc tcttccgcaa gcagcgccag cacatggagc gttgcgccga gcgagcccac
    14521 ccgcctgccg ccgggatcag caaccacgag cgtgcgggtc tcgggcgcga gcagaccgac
    14581 agcgcgtcgc cacgccaact gtgcgcggta gccggcggct tgcgcctcat gggccgcggt
    14641 caaaacaagc agatcaaaag acatgcatca acggtatcat gtcccgctaa aacccgtcaa
    14701 ccggatgcga gggcaaaacg agcatttctg caatccgagc ctgatacgaa aaagaaccgc
    14761 gattttttta tgcgcggtgt tgacttgcgg ggccgggggt gatactgtac gccgctcttt
    14821 cacgccacac ggggttctgc ggaatcgtcg cgtggcgtgg ggggggcggc gaaaaaagtg
    14881 atttttttcg ccgcaggggg ttgacagcat gcggttgatt ctgctaatgt ctttccccgc
    14941 gccgcaaatc ggcgggaaac cccgaaggcg gcatgagaag ctctttgaaa accggaagct
    15001 caagcgcaga cccgacggat accgatccgg aggggatgcg gaaaaacaga agcttgtgtt
    15061 tgtccggaat caattaagaa cagaacgcgc cgcggccttt cgtggctgtc ggcgcgcgac
    15121 ggatttttct tgagagtt
//
LOCUS       DAOSRB010000052        15000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4070_length_15000_cov_8.754968, whole genome shotgun sequence.
ACCESSION   DAOSRB010000052 DAOSRB010000000
VERSION     DAOSRB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15000
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_15000_cov_8.754968"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLZ74_04420"
     CDS             <1..822
                     /locus_tag="PLZ74_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR97599.1"
                     /translation="RAAVESARAAVATAKVVLKDRNRERQRMEKLFAEGSTTEQQRDL
                     AVTAYEQSVTGLAQAEAHLTHAQTAVGVLDVTLSEAFIHAPMDGVISAKSVDAGAMVN
                     ASTRIVQVMPLCELKFLIAIPGPYLHLLTTGKTVVSVRSDAVPDRVFEGLIARIHPAV
                     DPITRTATVEVRLANELGASGEWLLRPGLYAEGQIILEVKRDVVTLPADVALRRGPRF
                     LAFVVKDGHAETRELKIGARDGNVLEILAGLAVGEQVVIMGQHRLTDKIAVRITE"
     gene            921..4037
                     /locus_tag="PLZ74_04425"
     CDS             921..4037
                     /locus_tag="PLZ74_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOR97600.1"
                     /translation="MRLPELGVKRPIFTLMVFIAILVLGGVSLVYLPVDLFPEIETPS
                     VTVITQWPGASVEDVEGRVTRVIENQLSIVNNLNEVTSRTREGVSAITCMFEWGTDLE
                     EAANDIRDRVSISRRFLPDDIEEPIIYKFNSSSMPVLTFAATAQESWEKLFDIIEDDL
                     GDALKRLPGVGAVNVYGGLHRQINIDIDPMKLAGYNLTLAEIEETIARENLTLPAGSI
                     KSGVIEYTVRVPGEFEDPEEARNIVVKRNASGGAIYLRDVADVYDGFEEPTRLSGADG
                     KQSVIFAVQKRSGANTLKVCDSVLQAVEEYARFLPQDIRIVNVMNSSDFIRQSLSNVT
                     GTLLWGGLFVTLTTFVFLRTFRASLVIVLTIPFSLIIAFFFMFIRGWTLNIISLSSLS
                     VAIGMVVDNAVVVLENIDSYTRRGVKLREASMYATGEVGLAISASTLTTVAVFVPLIF
                     VTGMAGIFFGQLGGIITTTLMASLFCSLMLTPMLATYLLKEDRDAIGGGWLGRTLYRL
                     SEAGLQAIEEAYTRLLGRALRWRGVICALAFAVFCFSLALFPLIGSEFSPDTDSGDLT
                     VNFELGLGTRVEETARLCEEVRQLGLDLIGAEHVVNSRWRCGDSKFGSGRQGSHIGRV
                     EFKLTPMDRRPFNVRDAGRLLLDTLRARPEFVKTSMSTVNRQMGRFSGSGQPIVIEIL
                     GHDLAQTRRVAEEIKKLCDRTPGARDASVSMDDGKPELLVKVDRLKVSTLGLNVSDVV
                     ETIRTLFYGKEASDFREGENEYWFFLRLKESHRRELANILDSEIALPNGRRVRVDAIA
                     DVVQDLGPLDIERKDQERMVKVEVDYHERSIGEVVASIREGIAKEIVLPEATRITYGG
                     MVKEQTEAFADLIGLTILGLVLVYMVMAAQFESLVDPLVIFLALPFAFSGVLFGLYGF
                     GMTFNMFSFIGIILLVGTGVNNGIVLVDHINILRARGEGLTDAICHSGRQRLRPVLIT
                     TLTTVFGMLPLALAGGEGSETWKPMGVTVIGGLSFSTIVTLVLVPVLYSLFNRDARNP
                     KTQRKDPR"
     gene            4034..4909
                     /locus_tag="PLZ74_04430"
     CDS             4034..4909
                     /locus_tag="PLZ74_04430"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOR97601.1"
                     /translation="MKKCVWMALATLLLTSAVEAQILKPVPPADVQTLREAIGTRMTA
                     QPSKPRTVLVFWRCEGFIHTEAIEYGNKAFEIAAQQTRAFRVEFSSDYRALTDTNKLQ
                     RYDAVVLNNTTHLKTKDHPALVPNLTAFVKSGRGLCVIHAGADNFYNSPEAAEMVGGL
                     FDGHPWGGGGIWAFKLDEPGHPLNRAFGGQGFKIGDEIYQQKSPPYDRSKLRVLVSLD
                     LSDAKTASAKGQKRADKDFAVSWIRPYGAGRVFYTSFAHDRRAYLNKATLWHILDGLQ
                     YTLGDLKAEDTPVPK"
     gene            5182..6945
                     /gene="ilvB"
                     /locus_tag="PLZ74_04435"
     CDS             5182..6945
                     /gene="ilvB"
                     /locus_tag="PLZ74_04435"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOR97602.1"
                     /translation="MSKTPPTGTGHDSAPTMKSGAQCVIDGLVHAGCEVLFGYPGGAV
                     LDIFNCLYDAPFRFILARHEQGAVHMADGYARATGKPGCCLVTSGPGATNTVTGLATA
                     HMDGIPLICLTGQVALNMIGNDAFQEADVIGITRPVTKHNFLVRDADDLPDILAKAFY
                     IATTGKPGPVVIDLPKNVQKQKTAAVPPETVSMRAYHPDVPINPAEIEQFAEMINQAE
                     RPLLYVGGGAIAGDAPQRIAELAHRANIPVCTTLMGLGAFPEDDPLSLRMVGMHGSVA
                     ANYAVDHCDLLISAGARFDDRVTGKISAFASRAKIVHIDIDRSSIGKSVQTDLGVQGY
                     LDPVLTAVLPLVKPAEHAAWLEQVNRWKSRFPFAYPTPEGVLMPQYVIEQIYEVSQGR
                     ALITTDVGQNQMWATQFFRYTRPRSFISSGGLGTMGFGLPASIGVQIGRPDEQVICIT
                     GDGGAQMNFQELVVAVEHNLPLVVVILNNGYLGMVRQWQELFYGKRYAGVMLSQHGRL
                     PNEGIDENPVYLPDFVKLAEAHGAKTCRITRPDEVAPALRQALEERTTWVIECIVAPE
                     ANVFPIIPPGQHVDEMINRTV"
     gene            7012..7512
                     /gene="ilvN"
                     /locus_tag="PLZ74_04440"
     CDS             7012..7512
                     /gene="ilvN"
                     /locus_tag="PLZ74_04440"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOR97603.1"
                     /translation="MKHIINCLVENQPGVLARIVGLISGRGYNIETLNVGPTADPTVS
                     KMTIVVPGDAHVIAQVTHQLSKQVDVIEVVNMTNRRHIDRELLLVRVATQNHIMRAEL
                     VDLAALFMARVVAVQEDSVTIQMIGNQETVSDFLRLLKPYTVIDISRTGTIAVGRESS
                     DPTVLK"
     gene            7523..8383
                     /gene="aroE"
                     /locus_tag="PLZ74_04445"
     CDS             7523..8383
                     /gene="aroE"
                     /locus_tag="PLZ74_04445"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOR97604.1"
                     /translation="MRTAYRFAIIGHPVKHSLSPLMHEASFRALGIEAEYRCIDVPPD
                     TLGDRLAACRAEGFNGLNVTVPLKELALPLMDHLDASARLYGAVNTIRLDARGMTGFN
                     TDAEGFLTDLKLGYRLSPLSRTVLILGCGGAGRALATACVREGASDVLLADLDASRVN
                     RLAEEIARLLPDAAARVRPLPADPAAWREPSLSADLVVHCTPLGLHEDDPPALPPDAF
                     RPGQFLYDIVYTSRVTPTMRAAQAAGADTANGTGMLVRQGAAAFTVWTGLTADVAAMH
                     AALESRLYGT"
     gene            8795..10369
                     /locus_tag="PLZ74_04450"
     CDS             8795..10369
                     /locus_tag="PLZ74_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97605.1"
                     /translation="MRKKRIAVVGLVGLLFATSWCAGNDVVAFKDADKIKSWDAWDDH
                     KAKAAVTNILQKEKRNVTWQKLNAVNPGLKEIGRLATRTSEQVKSSKWSVGCETMDRD
                     YADWDSFKALIPMLGVKHARFFSGWAKTEQEKGKYDFTWLDPQIRECAAMSVKPWVCI
                     SYGNPVWGSDFRLGMRLKPVTDNPDAFDAWIRYTKALVERYKDVVDEWEIWNEPFGQA
                     REYAELFYRTAKAIREIQPAAKCFCTAISWNARDPMKSDYAMVLEKLKKENALDLGSY
                     FLYHPYSPVPEASYGHLAEPLRKFVKGYSANFDIMQGEVGCPSQLEFGHAMNGIEWTE
                     YSQAKWNLRRAIGDSARSIPSSVFTMIDLKYTFMLQSFGLVRSNLLKEFVYRRPSWYA
                     MSNIYSLFDDETLPQKNLVLKNFAIQKRADPRDQKPHDLACTLFTRHGKTLRFYWFCE
                     ARPSDRLDFDQIDLMIPERLDQPVWVDMITGRVFAIPETQIDRQEESLVLKNIPMWDS
                     PVLITARTTVMLKDEG"
     gene            complement(10479..11480)
                     /gene="gap"
                     /locus_tag="PLZ74_04455"
     CDS             complement(10479..11480)
                     /gene="gap"
                     /locus_tag="PLZ74_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOR97606.1"
                     /translation="MAIKVGINGFGRIGRMVFRAAVKNFSKEIEIVGINDLLDPDYLA
                     YMLKYDSVHGRFQGEVSVEGNNLIVNGKKIRLTSEKDPAALKWNEVGADIVVESTGLF
                     LTKETAEAHIKAGAKKVIMSAPSKDDTPMFVYGVNHKSYAGQAIISNASCTTNCLAPV
                     AKVLNDTFGIVKGLMTTVHATTATQKTVDGPSKKDWRGGRGILENIIPSSTGAAKAVG
                     KVIPELNKKLTGMAFRVPTSDVSVVDLTAVLAKETTYDEIKAAMKAAADGELKGVLGY
                     TEDKVVSTDFRGEAKTSVFDAEAGIMLDSTFVKIVAWYDNEWGYSNKVLEMAKVIAK"
     gene            complement(12126..14291)
                     /locus_tag="PLZ74_04460"
     CDS             complement(12126..14291)
                     /locus_tag="PLZ74_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97607.1"
                     /translation="MFRYAGTAAIASIILCVGVNAGMRPITDGHAEEWLCAGTWAGPA
                     GLTLLGIDADNEVRPCAGDVVPVKRFDETCWTNWTGIVRTDAGYVIAEKGGDFTWAKG
                     CSYAALYVKSDEAKTVDLEVTQSGYETRAFVNGVLVRETNRLWRKRKEKTVGITDQGN
                     ELTVDTEVGRLTSTLPIPLKAGVNFVMLKLYTRQQAGERVFFDAEFKDAAGLASDVND
                     PETDPVKHNALGQMYASVNVSAVANLPHPGEKLSVTTAIHYPWRKHKRPKSTKKDTRQ
                     EIVTPACPETFDAVVEQVVRDFDGRETARITYPITVPGTNMAALCTAGETGYYSVHTT
                     IKDTEGRRLFVFPADGFSVIGGTVSMRKRYRAGKMKTASCFYWVNGANSDGIFTWMDR
                     MGILHNVGGSASDTNIWSKAAAKGITMTADFLDPWGSENFIGKLAAVSNAAPYTRIFK
                     AWNEIDISRPQFKKTPELWTARTKMEYEAVKAIRPDAVYTGGSFVRVGNSDWFKGCLT
                     NGMERYVDVWDVHAYPKYPPKLDDRHVANSGNESGYGVETAYRSALGRENDKLFYLGE
                     CGARCSHGDDARRWQADTVAKLTAWAGSATNYLQIAHLVPWVKTSDGGDIPIAHFPAE
                     AALYTAVALTDGLPYERITTESKHIQAARFGSAVMAWTTHPGGEWFTFTPPEGELVTV
                     DVVGRVRPTTRTTDGCVSLHLSPSPVYLLSRTDYDRLTR"
     gene            complement(14305..>15000)
                     /locus_tag="PLZ74_04465"
     CDS             complement(14305..>15000)
                     /locus_tag="PLZ74_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97608.1"
                     /translation="GGGGGGGVLIVTNLVLSPGTYPVTVGAGGPGGGAGSANPIGGTT
                     SGESGGASIFANYYVPGGGYGGCCNVNGGIGASGGGGGRSNQSEASVPNAGSGREEWG
                     GFGGCKGTYDSSNVNAYGGSGGGAGGIGTREVGGDGMACDFSGELKWYGGGGGGGHGS
                     GGIHPKGGLGGGGDGGIALSVAAVEGTPGTGGGGGGGGHSVHTAAAGAMGGSGVVIIR
                     YEVLLNGFLISIR"
BASE COUNT         2980 a         4672 c         4353 g         2995 t
ORIGIN      
        1 cgggccgcgg tcgaaagcgc gcgcgccgcc gtggccacgg ccaaagtggt gctcaaagac
       61 cgcaatcgcg aacgccagcg catggagaaa ctgttcgccg aaggctcgac cacagaacag
      121 cagcgcgacc ttgccgtgac ggcctatgaa cagtcggtca ccgggctcgc ccaggccgag
      181 gcccacctca cgcacgcgca gacggcggtc ggcgtgctgg acgtgacgct cagcgaagcc
      241 tttatccatg cgccgatgga tggcgtgatc agcgccaagt ccgtcgatgc cggcgcaatg
      301 gtcaacgcct cgacccgtat cgtccaagtc atgcccttgt gcgaactgaa gttcctgatc
      361 gcgattccgg gcccctacct gcacctcctc accaccggta aaactgtggt cagcgtgcgc
      421 tccgatgcgg tgcccgaccg cgtgtttgag ggcctgatcg cccgcatcca cccggccgtg
      481 gatccaatca cccgcaccgc cacggtggag gtgcgcctcg ccaacgaact cggagcctcc
      541 ggggaatggc tgttgcggcc cggcctctat gccgagggcc aaatcatttt ggaggtcaaa
      601 cgcgatgtcg tcacgctgcc ggctgacgtg gccctgcggc gcggcccgcg tttcctcgcc
      661 ttcgtggtca aagacggtca tgccgaaaca cgcgagttga agatcggcgc gcgcgacggt
      721 aatgtgctgg agattcttgc gggccttgcg gtcggcgaac aggtggtcat catgggccag
      781 caccgcctaa ccgacaagat cgccgtgcgt ataacggagt gagccggtga tgatccctgc
      841 cgcaaacacg aaaacaggat acgtcccgaa cccgccgact cggcaccggt ccgggatccc
      901 gcaggaggag cgtcaacggc atgcgcttac ctgaactcgg agtcaaacgg ccgatcttca
      961 cgttgatggt cttcatcgcc atcctcgtgc tggggggggt ctcgctggtg tatctgccgg
     1021 tcgacctctt tcccgaaatt gaaaccccgt ccgtgaccgt catcacgcag tggccgggcg
     1081 cgagtgtcga ggatgtcgag ggccgcgtca cgcgggtcat cgaaaaccag ctcagtatcg
     1141 tcaacaacct caatgaggtg accagccgca cccgcgaggg ggtttccgcc atcacctgca
     1201 tgttcgagtg gggcaccgat ctggaagagg ccgcgaacga catccgcgac cgggtcagta
     1261 tctcgcggcg cttcctgccg gacgatatcg aggagccgat catctataag ttcaacagtt
     1321 cctcgatgcc ggtgctgacg ttcgccgcca cggcccagga gtcgtgggaa aagctgttcg
     1381 acatcatcga ggatgatctg ggcgacgcgc tcaagcgcct gcccggcgtg ggcgcggtca
     1441 atgtgtacgg cggcctccac cgtcagatca acatcgacat cgatcccatg aagctcgcgg
     1501 gctacaacct gacgttggcc gagatcgaag agaccatcgc ccgcgaaaat ctgacactgc
     1561 ccgcaggcag catcaagagc ggggtgatcg aatatacggt ccgcgtgccg ggcgaattcg
     1621 aggatccgga agaggcgcgg aatatcgtgg tcaagcgcaa cgcgtccggt ggcgcgatct
     1681 atctacggga tgtcgccgac gtctatgacg gctttgagga gcccacgcgc ctctcgggcg
     1741 cggacggcaa gcagagcgtc atcttcgcgg tgcagaaacg ttccggagcc aacaccctca
     1801 aggtctgcga ctccgtgctt caggcggtcg aagagtacgc gcgtttcctg ccgcaggata
     1861 tccgtatcgt caacgtgatg aactccagtg acttcatccg ccagtccttg agcaatgtga
     1921 ccggaacatt actgtggggc ggtctgttcg tcaccctcac gaccttcgtg tttttgcgca
     1981 cgttccgcgc ctcgctggtg atcgtgctga cgatcccgtt ctcgctgatc atcgcgttct
     2041 tcttcatgtt catccgcggc tggaccctca acatcatctc gctctcctcg ctttccgtgg
     2101 cgatcggcat ggtggtggac aacgccgtgg tcgtgctcga aaacatcgac agctacaccc
     2161 ggcgcggcgt caaactccgc gaggcctcga tgtacgccac cggcgaggtc ggcctcgcga
     2221 tctccgccag cacgctgacg accgtcgcgg ttttcgtccc gctgattttc gttacgggca
     2281 tggccggcat ctttttcgga cagctgggcg gcatcatcac caccacgctg atggccagcc
     2341 tgttctgctc cctgatgctc acgccgatgc tcgccaccta tttactcaaa gaggaccgcg
     2401 acgcgatcgg tggcggctgg ttgggccgca cgttgtaccg actcagcgag gcggggttac
     2461 aggcgatcga agaggcctat acccgtctgc tcggccgggc actgcgctgg cgtggcgtga
     2521 tctgcgcgtt ggcgttcgcg gtgttctgct tctccctcgc gcttttcccg ctgatcggct
     2581 cggagttctc gcccgatacg gattccggcg acctgaccgt caatttcgaa ctcggcctgg
     2641 gcacgcgggt cgaagagacc gcgcgcctct gcgaggaggt gcgccaactc gggctcgacc
     2701 tcatcggtgc cgagcatgtg gtcaactccc gctggcgctg cggcgactcc aagttcggtt
     2761 cgggccggca gggttcgcac atcggacggg tagagttcaa gctgaccccc atggaccgcc
     2821 gtcccttcaa cgtgcgggac gcggggcgtc ttctgctcga caccctgcgc gcccgacccg
     2881 aattcgtcaa aaccagcatg agcacggtca accgccagat gggacgcttc agcggcagcg
     2941 gccagcctat cgtgatcgaa atcctcggcc atgacctcgc ccaaacgcga cgcgtggcgg
     3001 aggagatcaa gaaactctgc gaccgcaccc ccggcgcgcg cgacgcgagt gtcagtatgg
     3061 acgacggcaa acccgagctg ctggtcaagg tggaccgcct gaaggtctcc accctcggcc
     3121 tgaacgtctc ggacgtggtg gagacgatcc gcacgctctt ctacggcaag gaggcgtccg
     3181 acttccgcga gggggagaac gagtattggt ttttcctgcg cctgaaagag tcgcaccgcc
     3241 gcgagttggc gaacatcctc gattccgaaa tcgcactccc caacggccgc cgcgtgcgcg
     3301 tggatgccat cgccgatgtg gtccaggatc tcgggcctct cgatatcgaa cgcaaggatc
     3361 aggaacggat ggtgaaggtc gaggtcgact atcacgaacg ttcgatcggt gaggtcgtgg
     3421 ccagcatccg ggaagggatc gccaaagaga tcgtgctgcc cgaggcaacg cgcatcacgt
     3481 acggcggcat ggtcaaggaa caaaccgagg ccttcgcgga tctgatcggc ctgacgattc
     3541 tgggtcttgt gctggtctac atggtgatgg cggcgcaatt cgaatcgctg gtcgaccccc
     3601 tcgtgatttt cctcgcgctg ccctttgcct tttctggagt ccttttcggc ctgtatggct
     3661 tcggcatgac cttcaacatg ttcagcttta tcggcatcat cctgctggtg ggcaccgggg
     3721 tcaacaacgg cattgtgctc gtggaccaca tcaacatcct gcgcgcgcgc ggcgaaggcc
     3781 tgacggacgc gatctgccac agcggccgcc agcggctgcg gcctgttttg attaccaccc
     3841 tcaccacggt ttttggtatg ctccctctcg ctttggcagg aggcgagggc tcggaaacct
     3901 ggaaacccat gggcgtgacc gtgatcggcg gactctcctt ctccaccatc gtcaccctgg
     3961 tgctggtccc ggtgctctac agccttttca accgcgatgc gcgaaacccc aaaacccaaa
     4021 gaaaggaccc aagatgaaaa aatgcgtctg gatggctctg gccaccctgc tcctcacctc
     4081 ggccgttgag gcccagatcc tcaaaccggt tccgcccgct gacgtgcaaa ccctccgcga
     4141 ggcgatcggg acccgcatga cggcgcaacc gtccaaaccg cgcaccgtac tcgtgttctg
     4201 gcggtgcgag ggcttcatcc acaccgaagc catcgaatac ggcaacaaag cgttcgagat
     4261 cgccgcgcaa cagacgcggg ccttccgtgt cgagttcagc agcgactacc gcgcgttgac
     4321 cgataccaac aagctgcaac gttacgacgc ggtcgtgctg aacaacacca cgcatctgaa
     4381 aaccaaggac catcccgcgc tcgtgccgaa tctgaccgca ttcgtcaaga gcggacgcgg
     4441 cctgtgcgtg atccacgccg gcgcggacaa cttctacaac agcccggaag ccgccgagat
     4501 ggtgggcggg ctctttgacg gccatccttg gggcggcggc ggcatctggg cgttcaaact
     4561 ggacgagccc ggccacccgc tcaaccgtgc cttcggcggc caggggttca aaatcggcga
     4621 cgagatctat cagcagaaat ccccgcctta cgaccgctcc aaactgcgcg tgctggtgtc
     4681 gctcgacctg tcggacgcga aaaccgcctc agccaagggc cagaaacgtg cggataagga
     4741 cttcgcggtg agctggatcc ggccctacgg cgcaggccgc gtcttctaca cctcgttcgc
     4801 gcacgaccgc cgtgcgtatc tcaacaaagc caccctgtgg catattctgg acggcctgca
     4861 gtacacgctg ggcgacctca aggcggagga caccccggtc ccaaagtgaa gacaaaaaaa
     4921 gacccgcccc ctcttgacag catccgctct tcaggataaa gttgcgcaaa tgagttcatg
     4981 gaagtccaaa gcgtttgggg tgaaccgcct ctctgttgcc gggcgggtta cgcccgttta
     5041 taccgtatca tggccgttcg cggcgggctt ctaaaggaat caggcaacac gcgatttctg
     5101 tttcaggaca agacctgccg ttggagagac ggcaggtttt ttcatttgag tgagacgtaa
     5161 gcaaggacaa gagaggtgaa catgagcaag acgcccccca ccgggacagg acacgattcc
     5221 gcgccgacga tgaagagcgg cgcacaatgc gtcatcgacg ggctggtgca tgcgggttgc
     5281 gaggtgctgt tcggctatcc gggcggcgcg gtgctggata tcttcaactg cctctacgat
     5341 gcgccgttcc gtttcatcct cgcgcgccac gagcagggtg ccgtgcacat ggcggacggg
     5401 tatgcccgcg caaccggcaa accgggttgc tgcctggtga cctcagggcc gggcgcgacc
     5461 aacaccgtga ccggcctggc gaccgcgcac atggacggca tcccgttgat atgcctcacc
     5521 ggccaggtgg cgctcaacat gatcgggaac gacgcgttcc aggaagcgga cgtgatcggc
     5581 atcacgcggc cggtgacgaa gcacaacttc ctcgttcggg acgcggatga cctgccggat
     5641 attctcgcca aggcgtttta catcgcgacc accggcaaac cgggacccgt ggtgatcgac
     5701 ctgcccaaga atgtgcagaa gcagaaaacc gcggccgtgc cgcctgaaac cgtgtccatg
     5761 cgcgcctatc atccggatgt gccgatcaac ccggcggaga tcgagcagtt cgccgagatg
     5821 atcaatcagg ccgagcgccc gttgctctat gtgggcggcg gcgcgatcgc gggcgacgcc
     5881 ccgcaacgga tcgccgaact ggcgcaccgc gcgaacattc cggtgtgcac caccctgatg
     5941 gggctgggcg cgtttccgga agatgacccg ctctcgttgc gcatggtggg catgcacggc
     6001 agcgtggccg cgaactacgc ggtggatcac tgcgatctgt tgatttcggc gggagcgcgg
     6061 tttgatgacc gcgtgaccgg caagatctcg gctttcgcca gccgggcgaa aatcgtgcat
     6121 atcgatattg accgctcctc catcggaaaa agcgtacaga ccgacctcgg cgtgcagggg
     6181 tatctggatc cggtgctgac cgcggtgctg ccgctcgtca aacccgcgga acacgccgcg
     6241 tggctcgaac aggttaaccg gtggaaatcg cgtttcccgt tcgcctatcc gacacccgaa
     6301 ggcgtgctca tgccgcaata cgtgatcgag cagatttacg aggtgagcca ggggcgcgcg
     6361 ctgatcacca cggatgtggg acagaaccag atgtgggcca cgcagttctt ccgctacacg
     6421 cggccgcgca gcttcatctc ctcgggaggg ctcggcacta tggggtttgg cctgccggcc
     6481 tccatcggcg tccagatcgg acgtccggac gaacaggtga tctgcatcac cggggacggc
     6541 ggggcgcaga tgaatttcca ggagttggtc gtggcggtcg agcacaacct gccgctcgtg
     6601 gtggtcatcc tgaacaacgg gtacctcggc atggtgcggc agtggcaaga gctgttttac
     6661 ggcaagcggt atgccggggt catgctgagt cagcatgggc ggctgcccaa tgaggggatc
     6721 gacgagaatc ccgtctatct tcccgatttt gtgaagctgg ccgaagcgca cggcgcgaaa
     6781 acctgccgca tcacccggcc cgacgaggtg gcgcctgccc tgcgtcaggc gctggaggaa
     6841 cggacgacgt gggtgattga atgcatcgtg gcgccggaag ccaatgtgtt ccccatcatt
     6901 ccgcccggcc agcatgtgga cgaaatgatc aaccgaacgg tgtaagcgga gggtgcctgc
     6961 acagccgctt gagacttgaa tccgatctgc ccagatgaag gaaaagacag tatgaagcat
     7021 atcatcaact gcctcgtgga aaaccagccc ggcgtgctcg cgcgcatcgt gggcctgatt
     7081 tccgggcggg gttacaatat cgaaaccctg aacgtggggc cgactgcgga ccccacggtg
     7141 tcgaaaatga cgatcgtggt gcccggagac gcgcatgtga tcgcccaagt gacccaccag
     7201 ctctcgaagc aggtggatgt gatcgaagtg gtcaacatga ccaaccggcg tcacatcgac
     7261 cgcgaactcc tgctggtgcg cgtggcgacc caaaaccaca tcatgcgggc cgagctcgtg
     7321 gacctcgcgg ccctgttcat ggcgcgcgtg gtggcagtgc aggaagattc ggtgaccatc
     7381 caaatgatcg gaaaccagga aaccgtgtcc gatttcctgc gcctgctcaa accgtacacg
     7441 gtcatcgaca tctcgcgcac cggcacgatc gccgtgggcc gggagagttc cgatcccacc
     7501 gtcctcaaat aaaggagact gcatgcgcac cgcatatcga ttcgcgatta tcgggcaccc
     7561 ggtgaagcac tcgctctcgc cgctgatgca cgaggcgtcg ttccgcgcat tgggcatcga
     7621 ggcggagtac cggtgcatcg acgtcccgcc ggacacactc ggcgaccgcc tcgccgcctg
     7681 tcgtgcggag ggctttaacg gcctcaacgt aacggttccg ctcaaagagt tggccctgcc
     7741 cctgatggat cacctcgacg cctccgcccg cctttacggc gcggtcaaca cgattcgact
     7801 ggacgcgcgc ggcatgaccg gattcaacac cgacgcggag ggcttcttga cggatctcaa
     7861 gttgggatac cgtctctcgc ccctgtcgcg caccgtcctg atcctcggat gcgggggtgc
     7921 gggccgggct ctggcgaccg cctgcgtgcg cgagggcgct tcggacgtgc tgctggcgga
     7981 tctggacgcg tcgcgtgtaa accgcctggc tgaagagatc gcccgcctgt tgccggatgc
     8041 ggcggcgcgt gtcaggcccc tgcccgcgga tcccgccgca tggcgggaac cgagtctcag
     8101 cgccgatctg gtggttcact gcacgccgct cggtctgcat gaggatgacc ccccggcact
     8161 gccgcccgat gcattccgtc ccggccagtt cctgtatgac atcgtctaca cgtcccgcgt
     8221 cacccccact atgcgggcag cgcaggcagc cggagcggat acggcaaacg gcacaggcat
     8281 gctcgtccgg caaggcgccg ccgcgttcac ggtctggacc ggactaaccg ctgacgtcgc
     8341 ggccatgcac gcggcgctcg aatcccgtct gtacggaacg tgagggcata aggagtgaat
     8401 tcctgaattc ccgaacgcaa tccgtcaaac agaaagacac cgtagttttt ttggtgcgtt
     8461 gacgatccgt caaatactgg acaccgttgg cctttttgct ctttagtgca gaataggcgc
     8521 ggcgcttcgc gccgacgtat tccattgtgg ctttccgtga catctcgttt ctcatgcgcg
     8581 ccaattctag cacggtgtcc agtatttgac ggatacgctt gatttctggg caccgcgatt
     8641 ttggctacgg tgtccatgac gatggcacac cgcaggccta ccgcgcacca accgattacc
     8701 ttgacgtttc ggatggagaa tgacaccatg gcgtcacgat tttggaacag cgggtttctt
     8761 acgtcggatc agttttggaa aggaaatgag agacatgaga aaaaagcgga tcgccgtagt
     8821 tggtttagtt ggtttactgt ttgcaacttc atggtgcgca ggcaatgatg ttgtcgcatt
     8881 taaagatgcg gacaagatta agagttggga cgcatgggac gaccacaagg cgaaagccgc
     8941 agtgacgaat atcctgcaga aggagaagcg gaacgtaaca tggcaaaagc tcaacgccgt
     9001 caatccgggt ctgaaggaaa tcggtcgcct cgccacacgc acgtcggaac aggttaaatc
     9061 gtctaaatgg tcggttggat gtgagacgat ggaccgcgac tatgccgact gggacagctt
     9121 caaggccctc atcccgatgt tgggcgtcaa gcatgcgcgg tttttcagtg gctgggcaaa
     9181 gaccgaacag gagaagggaa aatacgactt cacatggctc gatccgcaga tccgcgaatg
     9241 tgcggcgatg agcgtgaagc cttgggtttg catctcctac ggcaatcccg tgtggggtag
     9301 cgacttccgc ctcggtatgc gcttgaaacc agtgacggac aatccggatg cttttgacgc
     9361 ctggattcgc tacacgaagg cgctggttga acgctacaag gacgtggtcg acgagtggga
     9421 aatctggaat gagccctttg gacaggccag ggaatacgcc gaactcttct atcgcacggc
     9481 gaaggccatc cgtgaaatcc aacctgcggc caagtgtttc tgcacggcga tctcctggaa
     9541 cgcacgcgat ccgatgaaga gcgattacgc gatggtgttg gaaaagctaa agaaagagaa
     9601 cgcgcttgat ctcggatcct atttcctcta ccacccgtat agtcctgttc ccgaggcatc
     9661 ctacggtcat cttgccgaac cgctccgcaa gtttgtgaag gggtattccg ccaacttcga
     9721 catcatgcag ggagaggtgg gatgcccgtc ccaacttgag ttcgggcatg cgatgaatgg
     9781 gatcgaatgg acggagtatt cgcaggcgaa atggaatctg cgccgcgcga tcggcgattc
     9841 agcgcgatcg attccctcga gcgtcttcac gatgattgat ctcaagtaca cgttcatgct
     9901 tcagagtttc ggcctcgttc gctcgaacct gctcaaggag ttcgtctacc gccgcccttc
     9961 ctggtatgcg atgagcaaca tctattcgct cttcgacgac gagacactgc cccagaagaa
    10021 cctcgtgttg aagaacttcg ccattcagaa gcgcgccgat cctcgcgacc agaagccgca
    10081 tgatctcgcc tgcacactct ttacgcgcca cggcaagacc ttgcggttct actggttctg
    10141 cgaagcgcgt ccaagcgaca ggcttgattt cgaccagatt gatttgatga ttcccgagcg
    10201 actggatcaa cccgtatggg tggatatgat taccggacgc gtctttgcga ttccagagac
    10261 gcagattgat cggcaggaag agagtctggt gctcaagaac atccccatgt gggattctcc
    10321 ggtcctgatt acggcacgca cgacagtaat gttgaaggat gaaggatgaa agatgaagga
    10381 tgaaggatga aggatgaagg gcttgaacgt tggacgttca aaaacgccgc ccccgcatca
    10441 gcgggaacgg cgtcgtttac atatccggcg gagatcgctt acttcgcgat aactttggcc
    10501 atctccaaca ccttgttgga ataaccccac tcgttgtcgt accaggccac gatcttgacg
    10561 aaggtcgaat cgagcatgat gccggcctca gcgtcgaaga ccgaggtttt ggcctcgccg
    10621 cggaaatcgg tcgaaaccac cttgtcctcg gtatagccga gaacaccctt caactcgccg
    10681 tcagcagccg ctttcatggc cgccttgatc tcgtcgtacg tggtctcttt cgcgagcacc
    10741 gccgtcagat ccacgaccga gacgtcggag gtcggcacgc ggaaggccat gccggtaagc
    10801 ttcttgttca gttcgggaat caccttgccg accgccttgg ccgcgccggt gctcgacgga
    10861 atgatgttct cgaggatacc gcggccaccg cgccaatcct ttttggaggg accgtccacc
    10921 gtcttctgcg tcgccgtcgt ggcgtgcacc gtggtcatca ggcccttgac gatcccgaag
    10981 gtgtcgttca gaaccttggc gaccggcgcc aggcagttgg tcgtgcagga ggcgttcgag
    11041 atgatggcct gaccggcata gctcttatgg ttgacgccgt agacgaacat cggcgtatcg
    11101 tccttcgacg gtgccgacat gatcaccttt ttcgcgcccg ccttgatgtg cgcttccgcc
    11161 gtttccttcg tcaggaagag gccggtcgac tcaaccacga tgtcagcgcc gacttcgttc
    11221 cacttcagag cggcaggatc tttctccgac gtcaggcgga tcttcttgcc gttcacgatc
    11281 agattattgc cttcaaccga cacttcgccc tggaaccggc cgtgaaccga atcatacttc
    11341 agcatgtaag ccagataatc gggatccagc agatcattga tgccgacaat ctcgatctcc
    11401 ttcgagaaat tcttgaccgc cgcgcgaaac accatccggc caatacggcc gaaaccattg
    11461 ataccaacct tgatcgccat gttttgtact ccttcgttta ccttgagaat gccgtaacgg
    11521 gtattcctct ttgcaggcat gtcaacccga cgccggttac cggcccatac acgggaaaac
    11581 ttgcctgcgc cccttgcatg gatgggataa aaggataccc gttcgcatgg cgcgcgtcaa
    11641 ttgtgaatgc gcgttgattc gctctttttc catcgattgt acaagagcgg ggatccaacg
    11701 caagcgcagc ccgtaactca atttcgtctc acataaagga cacaagggtt tttcatgaca
    11761 tcccattccc cttcatgtcc tcgtgtggga aacaacttgc tttttttggc cttgtcggcc
    11821 gataaggcac gaatggggtt ccgggcaggc acggtttcaa tagccggact ccgcgtcacg
    11881 ggtggatcac gtttgattta cgcccatacc cgatcaggtt catttcacac cttgaaaggt
    11941 gtgaaattga cccgccagaa accgggcctt ccccgcataa tccgtgcgac taaaaaagcc
    12001 aaactccagc atttcacacc ttgaaaggtg tgaaattggg aaccggcgtg ttaccgggtc
    12061 gcaccttatg gagtgggagt gttaaggaac acccttctat cgcggtcaga aaggggcgtg
    12121 cccgtttatc gcgtcaaccg atcatagtcg gtgcgtgaga gcagatagac aggtgaaggc
    12181 gagagatgca gcgatacgca tccgtctgtc gtccgcgtag ttggacggac acgtccgaca
    12241 acgtccacgg taacgagttc gccctccggc ggcgtgaacg tgaaccactc tcctcccggg
    12301 tgtgtcgtcc acgccataac ggctgagccg aaccgagcgg cttgtatgtg cttcgattcg
    12361 gttgtgatcc gttcatacgg caggccatcc gtgagcgcca cagccgtgta aagggcggct
    12421 tcggccggga aatgggcgat cgggatgtca ccgccatccg acgtcttcac ccacgggaca
    12481 agatgcgcga tctgtaagta gtttgtggcc gatccagccc aggccgttaa ctttgcgacc
    12541 gtatccgcct gccaccgtct ggcatcgtca ccgtggctac accgtgcccc gcattcaccc
    12601 agataaaaaa gcttgtcgtt ctctcgtcct aacgcagagc gataagcggt ttccacgcca
    12661 taccccgatt cgtttccgga attcgcgaca tggcggtcat ctaattttgg cggatatttg
    12721 ggatatgcat gcacatccca aacgtccaca taccgttcca ttccattcgt gagacaccct
    12781 ttgaaccagt cgctgttgcc aacgcgaacg aacgaccctc cagtgtagac cgcatccggc
    12841 ctgattgcct tcaccgcctc gtattccatc ttcgtgcgcg ccgtccaaag ctccggagtc
    12901 ttcttaaact gcggcctcga tatgtcgatc tcattccaag ccttgaaaat ccgtgtgtac
    12961 ggcgcggcgt tgctcaccgc cgcaagtttg ccgatgaaat tttccgaccc ccaaggatcg
    13021 aggaaatcgg ccgtcatggt gatccccttc gccgccgcct tcgaccagat gtttgtgtcg
    13081 gacgccgagc cgccaacgtt gtggagaatt cccatgcgat ccatccacgt gaagatgccg
    13141 tcggagttcg cgccgttcac ccagtagaaa cagctcgcgg tcttcatctt ccctgcgcgg
    13201 taacgtttcc gcatggacac cgtaccgcca atcaccgaga agccgtccgc agggaagacg
    13261 aagagcctcc gtccttccgt gtctttgatt gttgtgtgga ccgaataata ccccgtctca
    13321 ccagccgtgc aaagggctgc catgttcgtg ccgggaaccg tgatcggata cgtgatgcgc
    13381 gctgtctccc ggccgtcgaa atcacgcacg acctgttcga caaccgcgtc aaacgtttcc
    13441 ggacatgcag gagttacgat ctcctgccgt gtgtctttct ttgtggattt tggccttttg
    13501 tgcttccgcc acggataatg gatcgctgtc gtcaccgaca gtttctcgcc cggatgcggc
    13561 aggtttgcca ccgcggaaac atttacgctc gcgtacatct gcccgagcgc attgtgctta
    13621 acgggatccg tttccggatc gttcacatct gaagcgagcc cggccgcatc tttgaattct
    13681 gcatcgaaga atacgcgctc gcctgcctgc tgccgggtgt agagcttgag catcacgaaa
    13741 ttgactcccg ccttgagggg gatcgggagc gtgctcgtaa gacgtccgac ctcggtatcg
    13801 accgttagtt cattgccttg gtcggtgatg ccgacggttt tttcctttcg tttccgccag
    13861 aggcgattcg tctctctcac gagcacaccg ttcacgaacg cgcgtgtttc gtatccgctt
    13921 tgagtcactt ccagatcaac tgttttcgcc tcgtccgatt tcacatacaa tgccgcatag
    13981 gaacaaccct ttgcccatgt gaagtcgccg cctttctctg cgatgacgta tcccgcatca
    14041 gttcgcacaa tccccgtcca gttggtccag catgtctcat caaaccgttt caccggcacg
    14101 acatcacccg cacacggccg cacttcgttg tcggcatcga tacccagaag cgtcagtccc
    14161 gccggtcccg cccacgtccc ggcgcacaac cactcctccg catgcccatc cgtaatcggc
    14221 ctcatccccg cattaacgcc aacacacagg atgatggaag cgatcgcggc ggtccctgca
    14281 tatcgaaaca taagaagcct tccattatcg gatgctgatc aaaaatccat ttaggagaac
    14341 ttcgtaacgg atgatcacga caccgcttcc acccatagcc ccggctgcag ctgtgtgcac
    14401 cgaatgcccg cctccgcctc ctccgccgcc tgtgcccggc gtaccctcta ccgctgcgac
    14461 agaaagcgcg atcccgccgt cgccgccgcc gccgagacct cctttgggat ggatccctcc
    14521 ggaaccgtgt ccgccgccac cgccgccgcc gtaccatttc agctcgccgg agaaatcgca
    14581 cgccatgccg tcaccaccga cttccctggt tccgatgccg cccgcgccgc cgccagaccc
    14641 gccgtatgcg ttcacattac tactgtcgta tgttcccttg caaccgccga acccgcccca
    14701 ctcttcacgt cctgatcccg cattgggcac actcgcttct gactggttgc tccgtccacc
    14761 gccaccaccg gaagcgccga tgccgccatt gacgttacaa caaccgccgt atccgcctcc
    14821 cggaacataa tagttcgcaa aaatcgaggc tccaccagac tcgccgctgg tagtgccgcc
    14881 aataggattt gcagatcccg cgccgcctcc cggaccaccg gcgccgaccg taacgggata
    14941 cgttcccggc gagagaacga gattcgtgac gatcaaaacg ccgccgccgc cgccaccgcc
//
LOCUS       DAOSRB010000053        14745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4158_length_14745_cov_7.126685, whole genome shotgun sequence.
ACCESSION   DAOSRB010000053 DAOSRB010000000
VERSION     DAOSRB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14745
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4158_length_14745_cov_7.126685"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..358)
                     /gene="infA"
                     /locus_tag="PLZ74_04470"
     CDS             complement(137..358)
                     /gene="infA"
                     /locus_tag="PLZ74_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOR97609.1"
                     /translation="MSSEDAIEVIGVVVKILPATMYRVRLENGHEVLAHISGKMRKHF
                     IKIAAGDRVTLEISPYDLTKGRIIYRHKA"
     gene            440..1381
                     /locus_tag="PLZ74_04475"
     CDS             440..1381
                     /locus_tag="PLZ74_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97610.1"
                     /translation="MTCEAGSDRVVKWADADSKDIIFSNVNYQALSTPNAATYKTNHI
                     NGLSTVYFDHSHIRYLAGGVPCSLRTYFGVVAHGHDATPFGILDGVFGQGGVDAGIRI
                     HNGTSYSDPIALTDRVNGDAGSAFAKGTPHLFSRVYPGNMKWDSPALSDYSPHKRPMS
                     GDICEIVVYGRVLGETERSLVEDYLMAKWGLKNLPDPAKYADVLPTGTDLTVASAATL
                     DLNGNDQTLASLDLSGTLTNSVERVATLTLSGVSFVRAGASFGAPNLDLVLSDDAVLD
                     LGGGTFTIRRLTGRRVQVINGVLNETKPLLDFLLIIR"
     gene            1485..2816
                     /gene="dnaX"
                     /locus_tag="PLZ74_04480"
     CDS             1485..2816
                     /gene="dnaX"
                     /locus_tag="PLZ74_04480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOR97611.1"
                     /translation="MAYEVLARKWRPQQFADVVGQEHVTRTLKNAIESGRVAHAYLFV
                     GPRGIGKTSLARIFAKALDCEKGPTATPCCECPLCREIAAGNALDVLEIDGASNNGVD
                     QVRDLRDQIQFAPTHGRFKIIIIDEVHMLSTAAFNALLKTLEEPPPHVKFIFATTEGD
                     KVLPTIISRCQRFDLRRIQTPLIVERLRHICNAERIRIDEDALLAIARGAEGGMRDAL
                     SALDQLISFKGGALTEEDVLAVFGLVSRASLEGLATAVLTGDMAEILRLVDQFDSAGK
                     DMRRLVVELMGHFRNLLVYQQVGEQMSTLDATPEQIRSLAAQAAHADSSRVLQIADQL
                     GEMEGRLRFALSMRTLIELSLIRCARIARAVSLEEVLKRLNALRGSDPTPAAAPSAAS
                     PVQSGAAAAGPLPGVAHDDPALKQVMQLFGGEVVDVDDGKGRSAQQKSSGA"
     gene            2856..5561
                     /locus_tag="PLZ74_04485"
     CDS             2856..5561
                     /locus_tag="PLZ74_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOR97612.1"
                     /translation="MLAVVAGCAAAGAIPHRVFVLENGLRLVVHEDRKVPIVAVQVAY
                     HVGSKNERPGRTGFAHLFEHLMFNGSEHFDDDYFKALERVGATGINGTTNEDRTHYFQ
                     TVPREALDTALWMESDRMGHFLGALTQERLEEQRGVVLNEKRQGDNQPYRIAWRLITR
                     HAWPLGHPYSWEVIGSEVDLAAASLQDVRAWFRDFYGAANAVLVVAGDVSAEEALQKV
                     RHAFGDIPPGPPVARPVVDVVRRTGARRMRVQDRVPQARLYKVWTIPQYGTTEGNRLT
                     LAAEALAAGKSSRLYKRLVYDAQLATEVHAWADLREIAGQFGVVITVRPGVSLAEAER
                     AADEEIARFLREGPTRRELERIQARIEADFIRQSEQVGGKADMLIRDLLFTGDAAYHE
                     KRMREWREATPRQVVETAREWLSDGAFILEVEPFPAYRVATAGADRSALPLPSELPEA
                     AFPAVRRATLSNGLRVVLVERHETPLVLCTLSVGAGFAADACAQRGTATLMADLLDEG
                     TRHLSALELGRELERLGAALSTGCAPDATLVSLNTLSRHLDRALDLYAEVILHPAFPE
                     EDFQRLRHQRLAQIRREKANPRGVAMRVMPRLLYGERHAYGQGFSGSGEEESVAGLTR
                     DDLERFYRQEFKPNRATLVVAGDVTLEALLPRLERRFGSWKPGEVPDRSVAEARPALR
                     PSVWLIDRPAAPQTLIVAGGIAPPKRHPLDPAIEALNTLLGGAFTSRVNMNLREEKHW
                     SYGARTYLTEVCGPRAFLCIAPIQTDRVADAMRELDREFRGITGTNPPTAEELRKAVA
                     HHTLQLAGRWETLGAVVGALEQVVRFDLPEEYYSVYAGTMRALTLPEVTRAAAEVIRP
                     ESLVWLVVGDRTQVEEPLRRLDWGPVNVLDADGKPVNGTVGSVSR"
     gene            5748..6623
                     /locus_tag="PLZ74_04490"
     CDS             5748..6623
                     /locus_tag="PLZ74_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOR97613.1"
                     /translation="MKNVIVLSACICFSCVGPRCVWGSDLVRPGETQESAKRGQSKGE
                     PTNAKEAADKKSATAAAIDAKYAEIVAKLPPEEQAWERTLQANLGGFYLPRYKEGRVR
                     GREQAWDYVKDVPGLPRVLLIGDSVSRGYTLATRKALVGKANVHRAPENCGPSANGLK
                     KLDIWLAGEKWDVIHFNFGIHDRATPPDVYRQRLETLVTRMKATGAKVIWASTTPIPP
                     DTQDGPKASEAIVEKNRIAAELMTRHGVAINDLFTFITPHLSKVQNPKDVHFKGEGYD
                     LLGKRVAEAITAALK"
     gene            6758..8290
                     /locus_tag="PLZ74_04495"
     CDS             6758..8290
                     /locus_tag="PLZ74_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOR97614.1"
                     /translation="MMKANRKITMSGTFIGVLGLSGWVACGAELDGVMSEAYWKLWNP
                     EVQVRIDRDIEKYRKADATLVLEGVAPGVDVKVEQLSHDFIFGAHIFNFNQLGSFERN
                     RKYKELYGTLFNSATIAFYWKKFEMEPGKLRFAEEERDTEAYWNSVKEPKKEPHWRRP
                     ATDPVVAFCESKGIRLHGHPITWGNKTWHYPEWLMERNCPAEEKAEIQKLGKDGLQKL
                     SPAEIAALLPVYTREINRRFTTRVEELARHYQGRIHSWDVVNESATDFSKGMMIPGDV
                     ICKSRYGLMPGDYTFASFQVAQREFPGTVKLNINDYLNTEAYVRQVKDLMARGCRIDI
                     MGSQMHLFNPRQCLDIAAGKPIESPDQVWSKMALLSQAKLPIHLSEITITSPGGDEKG
                     CKIQAVIARNLYRLWFSIERMMGITWWNVVDDCGAPGEPSVSGLFTRDMQPKPAFHAL
                     DKLIQSEWKTKLTCKTGPDGRVAFRGFKGRYRVSWRDAAGQTHSRGFHLSKDGDGILD
                     PL"
     gene            complement(8292..8678)
                     /locus_tag="PLZ74_04500"
     CDS             complement(8292..8678)
                     /locus_tag="PLZ74_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOR97615.1"
                     /translation="MTTFDVQTSLHSQVVNITDSIREAIKSLGITDGVVTAFVPHTTC
                     GLTVTEGDDPTVTADLLRQMDELVPWDRPYYTHTGGNAAAHIKSALIGTSLHLIVKGG
                     TLQLGMWQGIFLCEFDGPRIRHVWVR"
     gene            complement(8852..10225)
                     /locus_tag="PLZ74_04505"
     CDS             complement(8852..10225)
                     /locus_tag="PLZ74_04505"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR97616.1"
                     /translation="MIRPSLPVEYFERNRETILKEWFALLRLPTVGTDPKRLGECARA
                     AAWLKVFLRKLDFSVDIITPESGHPVPVVLAERPGNGTSTVLFYGHYDVQPEDPLEAW
                     LTPPFEPTLINGRVYARGAQDNKGQVFGFLQGVKALIEAGVPLPTLRIVIEGQEESGS
                     EGLTALAPSLRQRLQADVMMVCDTSCGPENQPAIIAGLRGVQHFTLTLNGPAYDLHSG
                     IHGGVAPNPAQGMARLLASLHTPDGAIAVAGFRDRVRPPEAEELEHARRAAAPAENYE
                     AEIGCPPVGGDRRLDPVARGSFEPTIEINGIHGGYGGPGSKTVIPSTVLAKLSMRLVP
                     DQRPDEVFDAVRSHLEAHCPRGLKLSLTEICPGAPGFRLELHSPLFRLAADVLERLDN
                     RGALFRWEGASIPVVALLRQVSGAAPLLVGFGREEDKIHCPNESFGLDQFERVMTWSA
                     MMLSELA"
     gene            10460..12544
                     /gene="fusA"
                     /locus_tag="PLZ74_04510"
     CDS             10460..12544
                     /gene="fusA"
                     /locus_tag="PLZ74_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR97617.1"
                     /translation="MSRTIPLDQIRNIGIMAHIDAGKTTTSERILFYSGKNYKLGEVH
                     EGAATMDYMVQEQERGITITSAATAVSWRDHQISLIDTPGHVDFTAEVERSLRVLDGA
                     IALFCAVGGVQPQTEQVWRQSEKYEVPKLCMVNKMDRIGANFFNVVNEIRYTLGGNPV
                     VMVYPMGVADSFRGVVDLLTMKALVFDEEDRGVRVLVEEVPEEFREECERLRVEMMEQ
                     AAEQDDALMAKYFETGELSIEEIKSGLRKGTINRKLAPTYCGTAFKNKGVQPLLDGVI
                     DYLPSPADIGSVRSADNPENLRSPDENEPFAGLAFKILSDKHMGKITFVRLYSGTLRV
                     GDEVLNSTMEQPGRIGRLFRMHAARQEALEAAYAGDIVAVVGLSKTRTGDTICDPKHP
                     ILLESIEFPAPVISIAIRPASRGDNEKLGNALYSLAQEDPTFVVRYDQETGETIISGM
                     GELHLEVIVDRLRREYGVACEVGKPEVAYRETIRHMVDGEYKHVKQSGGRGQYAHVCL
                     RLEPQEPGRGFSFVNEVKGGVIPNEYIPAVEKGVIKAMENGPCGGFPVVDVKVVVHFG
                     SYHAVDSSEFAFVECARQCFKNLFLKGNPQLLEPIMSVEVAVPEDYMGAATGSICQRR
                     GRVEGMDEKAGSKLIRGLVPLGEMFGYSNSIRTLTQGRGTFSMTFERYEGVPFELAET
                     IIEKRKKEGKVR"
     gene            complement(12672..13439)
                     /locus_tag="PLZ74_04515"
     CDS             complement(12672..13439)
                     /locus_tag="PLZ74_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR97618.1"
                     /translation="MSGHSKWSTIKHKKGAADAKRGKIFSKIAKELTVVAKAGGGDPN
                     TNPTLRTLITKAKAVNMPNDNIDRAIKKGTGELAADVLEEITYEGYAQGGVGLVVKVL
                     TDNKNRAAAEVRNIFKKNGSDFATLGSVSRGFERKGTIMIPAAEGLTEDKVMEVVLIA
                     GADDMESEEDGFTVTCSPAAFSEVCAALEAAEIAFDQENSLVGLVAQTLVPVKELAVA
                     KAVNKFVDALEENDDVQDVFTNMDIDDALVEQLEAEA"
     gene            complement(13494..14486)
                     /locus_tag="PLZ74_04520"
     CDS             complement(13494..14486)
                     /locus_tag="PLZ74_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOR97619.1"
                     /translation="MGNGERGTQDEKNFGVLSSAFGVLSSCRLCPRACGVDRLAGQRG
                     YCRAGVLPHVFRYGAHFGEEPPITGTRGSGTIFFSHCTMRCIYCQNHPWSQAGHGDDF
                     TVEALRDILEQLARQGCHNWNLVSPTPWLPQIREAAEPLLRTGIALPFVYNTSGYESL
                     ETLEAFADLADIALIDLRYARAESAAEGSDARTYVEAARGALRWFWERLGPLETDASG
                     IAVRGVICRILALPGRIAEAVENLEWLARHTGSGIHVSVMSQYTPLHRALQTPGWDRK
                     VTADEYAVLVEAAEDLGLGNGWIQEFEGEAPADLLGQNMPAGEGAVGAGSNTPV"
BASE COUNT         2859 a         4268 c         4757 g         2861 t
ORIGIN      
        1 cagcgtgatc gggatcgaac gtgagggcag gacccttgtc aatccgacgc ccgacgaaag
       61 gttggagccc ggcgatgtgc tgctgctgct gggggatgac aggcaaattg ccgaggcccg
      121 gcggctgatc ggctgatcag gctttgtggc ggtagatgat gcgccccttg gtcagatcat
      181 aaggggagat ttccagtgtc accctgtcac cggcggcgat cttgatgaag tgtttccgca
      241 tcttgccgga gatgtgggcc aacacctcgt ggccgttctc cagacgcacc ctatacatgg
      301 tggccggtaa gattttgacc acaacaccga ttacttcaat ggcatcttca ctggacataa
      361 cactccgtct gattggtctg aaacaattac gtgcaccacc ataacacgcg gccgcaggat
      421 tgacaagccg tggcgttacg tgacatgcga agcgggttcg gaccgcgtgg tgaaatgggc
      481 cgacgcggac agcaaggaca tcatcttctc gaatgtgaac taccaggccc tgtcgacgcc
      541 gaacgccgcg acctacaaga cgaaccacat caacggcctg tcaacggtct atttcgatca
      601 ttcccacatc cgatatctgg ccggcggcgt gccctgcagc ttacgcacgt atttcggtgt
      661 cgtcgcgcac ggacatgatg cgacgccctt tgggatactt gacggcgtct tcggccaggg
      721 cggcgtggac gcgggcatcc gtatccacaa cggcacgtcc tacagcgatc cgatcgccct
      781 gaccgaccgt gtcaacggcg acgcgggcag tgcgttcgcc aaggggacgc cacatctgtt
      841 ttcgcgcgtt taccccggca atatgaaatg ggactcgccg gctctcagtg actattcgcc
      901 gcataagcgt ccgatgtccg gcgacatctg cgagatcgtc gtctatggcc gggtgctcgg
      961 ggagacggaa cgcagtctgg tcgaagatta cttgatggcg aagtggggac tcaaaaacct
     1021 gccggatccg gcgaagtatg cggacgtcct gccgacgggg acggacctga ccgtggcttc
     1081 tgcagcgacg cttgacctga acggcaacga ccagacgctg gcgtcgctcg atctctccgg
     1141 cacgttgacg aacagcgttg aacgcgtggc gaccctgacg ctctcgggcg tttcttttgt
     1201 gcgcgcaggc gcgtccttcg gtgccccgaa ccttgatctg gtgctgagcg atgatgccgt
     1261 ccttgatctg ggcggcggca cgttcacgat ccgccgcctg accgggcgtc gggttcaggt
     1321 gatcaacggt gtcctgaacg agacgaagcc gcttctggat ttcctgctga tcatccgctg
     1381 acaaaaaaag acgcaaaggg acgatgtgtc cttccaagac tcggtgccga aggcgcgaga
     1441 ttccctttgc ttccctcggg gtaaacggta aactgttttc cgttatggct tatgaagtac
     1501 tcgcacgcaa atggcgtccg caacagttcg cggatgtggt cggtcaggag cacgtcaccc
     1561 gtactctgaa gaacgcgatc gaaagcggcc gcgtggccca cgcgtatctg tttgtcggcc
     1621 cgcgcgggat cgggaagacc tccctcgcgc ggatttttgc caaggcgctc gattgtgaga
     1681 aaggccccac cgccacaccc tgttgcgaat gtccgctctg ccgcgagatc gcggcgggga
     1741 atgcgctgga tgtgctggaa atcgacggtg cctccaacaa cggggtcgat caggtgcgcg
     1801 atttgcgcga ccagatccag tttgcgccga cgcacgggcg cttcaaaatc atcatcattg
     1861 acgaagtcca tatgctgagc acggccgcgt tcaacgcatt gctgaagacg ctcgaagagc
     1921 cgccgccgca tgtgaaattc atttttgcga cgaccgaagg cgacaaggtg ctgccgacca
     1981 tcatctcacg ttgccagcgt ttcgatctgc gccgtatcca gacgccgctg attgtcgagc
     2041 ggttgcgcca tatctgtaac gccgagcgca tccgtattga cgaggacgcc ctgctggcga
     2101 tcgcgcgtgg ggccgaaggc ggcatgcggg atgcgctgtc cgcgctcgat cagctgatct
     2161 cgtttaaagg cggggcgctt actgaagagg atgtgctcgc ggtgttcggg ctggtctccc
     2221 gcgcctcgct ggaagggctc gcaactgccg tgttgaccgg tgacatggcc gaaattttgc
     2281 ggttggtgga tcagttcgac agtgcgggca aagacatgcg gcggttggtg gtggagctca
     2341 tgggacactt ccgcaacctg ctggtgtatc agcaggtggg cgagcagatg tccacactcg
     2401 atgccacgcc ggaacagatc cgcagtctgg ctgcgcaggc cgcacacgcg gattcgtcac
     2461 gggtgttgca gatcgctgac caactcgggg agatggaggg acgcctgcgc tttgcgctct
     2521 ccatgcgcac cttgatcgag ttgtcgctga tccgttgcgc gcgtatcgcg cgggccgtct
     2581 cgctggagga ggtgctcaaa cggctcaacg cgttgcgggg gtcggacccg acccccgctg
     2641 cggcgccgtc cgcggcgagt cccgtccagt ccggtgccgc cgccgctggg ccgttgccgg
     2701 gtgtggcgca cgatgaccct gcgctgaaac aggtcatgca actcttcggg ggtgaggtcg
     2761 tggacgtgga cgacggcaag ggacggtccg cgcagcaaaa gagttcggga gcgtaaggta
     2821 tggggagtcg gcaacgcagc atcgggattg catgcatgct ggccgttgtc gcggggtgtg
     2881 ctgcggcggg tgcgataccc catcgggtct tcgtgttgga gaacggtctg cgactggtgg
     2941 tgcatgaaga ccgcaaggtg ccgatcgtgg ctgtgcaggt tgcctaccat gtcggatcca
     3001 agaacgaacg tcccggccgc accgggttcg cgcacctgtt cgaacacctg atgttcaacg
     3061 gcagcgaaca tttcgacgac gattatttca aggcgcttga acgggtcggc gcgaccggga
     3121 tcaacgggac gaccaatgaa gaccgcaccc attattttca gacggtgccg cgcgaagcat
     3181 tggataccgc gctctggatg gagtccgacc gcatggggca ctttcttggc gcgttgactc
     3241 aagagcggct cgaggagcag cggggggtgg tgctgaacga aaaacggcag ggggataacc
     3301 agccgtaccg catcgcctgg cgactcatta cgcggcacgc ttggcctttg gggcatccgt
     3361 attcatggga ggtgatcggt tcggaggtgg atctcgctgc cgcatcactt caggatgtcc
     3421 gcgcgtggtt ccgtgatttt tacggtgcgg ccaacgcggt gttggttgtg gcgggcgatg
     3481 tctccgcaga ggaggcgttg cagaaggtcc ggcacgcctt tggcgacatc cccccgggac
     3541 cgccggtcgc gcgccccgtc gtggatgtgg tgcgacgcac cggggcccgg cgcatgcggg
     3601 tgcaggaccg tgtgccgcag gcacgcctct acaaagtctg gactatcccg caatacggca
     3661 cgacggaagg caaccgtctc accttggctg ccgaggcgct cgcggcaggc aaatcctcac
     3721 ggctttacaa gcgtctggtg tatgacgccc aacttgccac cgaggttcac gcttgggcgg
     3781 atctgcgcga gatcgcgggg cagttcgggg tggtcatcac cgttcggccg ggcgtttctc
     3841 tggcagaggc cgagcgggcg gctgatgaag agatcgcccg gtttctccgc gaggggccga
     3901 ctcgcaggga attggaacgt atccaagctc gtattgaggc ggatttcatt cggcaaagcg
     3961 aacaggttgg cggtaaagcg gacatgctga ttcgtgatct gctgttcacc ggagatgcgg
     4021 cctatcacga aaagcggatg cgggaatggc gcgaggccac accccggcag gtggttgaga
     4081 cggcacgcga gtggctgtcc gatggtgcct tcattctgga ggtcgagccg ttccccgcgt
     4141 atcgcgtggc cacggccgga gcagaccgca gcgcgctgcc gctgccatcg gaactgcccg
     4201 aggccgcctt tcctgcggtt cgtcgggcca cgctctccaa cgggttgcgg gtggtgctgg
     4261 tcgagcggca tgaaactccg ctggtacttt gtacgctgag tgtgggggct gggtttgcgg
     4321 cggatgcgtg cgctcagcgc ggtacggcca cactgatggc ggatctgctg gatgaaggca
     4381 cgcggcacct ctcggcactt gaactcggac gggaattgga acggttgggc gcggcgttga
     4441 gcacggggtg cgcgcctgac gcgacgctgg tctcgctgaa cacgctctcg cgccatttag
     4501 accgcgcctt ggatctctat gctgaagtga tcctccaccc cgcctttccc gaggaggatt
     4561 tccagcggct gcggcaccag cggttggcgc agatccggcg ggaaaaggct aatccccgcg
     4621 gggtggcgat gcgggtgatg ccgcgcctgc tttacggtga gcggcacgcg tacggacagg
     4681 ggttcagcgg tagtggcgaa gaggagtcgg tcgccgggtt gacccgcgat gatctggagc
     4741 gcttctaccg gcaggaattc aagccgaatc gcgcgacact ggtggttgcc ggggatgtca
     4801 cgctcgaagc gctgctgccg cgtttggaac gccgcttcgg ctcatggaag cccggagagg
     4861 ttccggacag gtccgtggcg gaggcgcgtc ccgcactccg accctcagtc tggctgatcg
     4921 accgccctgc cgcgccgcag acgctgattg tcgccggcgg gatcgcgccg cccaagcgcc
     4981 atccgctgga ccccgcgatc gaagcgctga acacgctgtt gggtggtgcc ttcaccagcc
     5041 gggtgaatat gaatctccgg gaggagaaac actggagcta tggcgcgcgg acctacctca
     5101 ccgaagtgtg cggcccgcgc gcgttcctct gtatcgcgcc gatccagact gaccgcgtgg
     5161 cggacgcgat gcgcgaattg gaccgcgaat tccgcggaat cacggggacg aaccctccga
     5221 ctgcggagga attgcgcaag gcggtggcgc atcatacgct gcaactcgcg gggcgttggg
     5281 aaacgttggg cgcggtcgtc ggtgcgctcg aacaggtggt ccggttcgac ctgccggagg
     5341 agtattactc cgtgtatgcc ggaaccatgc gcgcgctcac gttgccggag gtgacgcggg
     5401 cggcggcaga ggtcatccgg cccgagagtc tggtctggtt ggttgtcggg gaccgtactc
     5461 aggtcgaaga gccgctgcgc cggctcgatt gggggccggt caatgtattg gatgcggatg
     5521 ggaaaccagt gaatggaacg gtcgggtcgg tttcgcgatg aattccgtca accggttcgg
     5581 gaggggataa catgagtttg aaacgtcgcg ctatccgtat cacccgcacg actgttgcga
     5641 aggcgtgcgg gcttactctc cagacaagag gcagccatcc atgcgtaccc gctgttctcg
     5701 gatggtgatg gatttccgca aactcaaacc aagctatggg agacagaatg aaaaacgtaa
     5761 tcgtgctatc cgcttgcatc tgcttttcgt gcgtcgggcc acgctgcgta tggggctccg
     5821 acctggtccg gcccggcgaa acgcaagagt ccgcgaaacg cgggcagtcg aaaggtgagc
     5881 ccacgaacgc caaggaggcg gcagacaaga agagcgccac ggctgcggcg atcgacgcga
     5941 aatacgcgga gattgtggcc aagctgccgc cggaggaaca ggcatgggag cgcacgctgc
     6001 aggcgaactt gggaggcttc tacctgccgc gctacaagga gggccgcgtc cgtggcagag
     6061 aacaggcgtg ggactacgtg aaggacgttc cgggtttgcc gcgtgtgctg ctcatcggcg
     6121 attccgtgtc acgaggctac acactggcaa cccgcaaggc gctggttggc aaagccaacg
     6181 tccatcgtgc gccggaaaac tgcggtccat cggccaacgg gttaaagaaa ctcgatattt
     6241 ggctcgctgg cgaaaaatgg gacgtgattc acttcaattt cgggatccat gaccgcgcca
     6301 cgcctccgga cgtttaccgc cagcggctgg agacgctcgt cacccgcatg aaagcgaccg
     6361 gtgccaaggt catctgggcc agcaccacgc cgatcccgcc ggatacccaa gacgggccga
     6421 aggcctccga ggccatcgta gagaagaacc gcattgcggc cgagttgatg acgcggcatg
     6481 gtgtcgccat caacgacctg ttcaccttca tcacaccgca cctctcaaag gtgcagaacc
     6541 cgaaggatgt ccacttcaaa ggcgaagggt atgacctgct cggcaaacgg gttgccgagg
     6601 cgatcaccgc cgccctgaag tgaaggtgcc cgtcaaaaat caagaacacg gacttgtgcg
     6661 caaggacagg aaggctgtag aatgagacat gaaggacgga ctttgacgcg tcgtgcgtct
     6721 ggctgcatcc tttgattgtg ggcagaaagg cggatggatg atgaaggcga accggaagat
     6781 cacgatgagc gggacgttca tcggcgtgct gggcttgtcg ggctgggtgg cctgcggcgc
     6841 cgaactggac ggagtaatga gcgaggcgta ctggaaactt tggaatccgg aggttcaggt
     6901 ccgcattgac cgcgatatcg agaagtaccg caaggcggat gcgacgctcg ttctggaggg
     6961 ggtggcgccc ggagtggatg tgaaggtcga gcaactctcg catgacttca tcttcggtgc
     7021 gcatatcttt aatttcaatc agctcgggtc tttcgagcgt aaccggaagt acaaggagtt
     7081 gtatgggacg ctgttcaatt cggcgacgat tgcgttctac tggaaaaaat tcgagatgga
     7141 gcccggaaaa ctccgttttg cggaagagga gcgcgacacg gaagcctatt ggaacagcgt
     7201 gaaggaaccg aaaaaggagc cgcactggcg tcgcccggct acggatccgg tggtggcctt
     7261 ctgtgaaagc aaaggaatcc gtctgcacgg tcatccgatc acgtggggga acaagacctg
     7321 gcactatccg gagtggctga tggagcgcaa ctgtcccgcg gaggaaaagg cggagattca
     7381 gaaattgggc aaggacgggt tacagaagct gtcccctgcg gagattgcgg cgctacttcc
     7441 cgtttacacg cgtgagatca accgccgttt cacaacgcgc gtggaagagc tcgcccgaca
     7501 ctatcagggg cgcatccaca gttgggatgt ggttaacgag agcgcgaccg atttcagcaa
     7561 gggcatgatg atccccgggg acgtgatttg caagagccgc tatggactga tgcccggtga
     7621 ctacaccttt gcctcgtttc aggttgcgca acgtgagttt cccggaacgg tgaaactcaa
     7681 catcaacgat tatctcaaca ccgaagctta cgtgagacag gtcaaagacc tgatggcgcg
     7741 cggctgccgc atcgacatta tgggttcgca gatgcacctc ttcaatcccc ggcagtgcct
     7801 cgacatcgcg gcgggcaagc cgattgagtc accggatcag gtttggtcca agatggcgct
     7861 gctctcccag gccaagctgc cgattcacct gagcgagatc accatcacct cgccgggagg
     7921 cgatgaaaag ggatgtaaga tccaggcggt gattgcgcgt aacctctacc gcctctggtt
     7981 cagcatcgaa cgtatgatgg gcatcacctg gtggaatgtg gtggacgatt gcggcgcgcc
     8041 cggcgagccg tcggtgtcag gactctttac ccgggacatg cagccgaaac ccgccttcca
     8101 tgcgctggac aagctgattc aaagcgagtg gaagacgaag ctgacctgca aaaccgggcc
     8161 ggacggccgg gtcgccttcc gcggcttcaa ggggcggtac cgcgtatcct ggcgtgacgc
     8221 ggccgggcag acgcacagcc gcgggttcca tctgtcgaaa gacggggacg gaatcctcga
     8281 tccgctttag cttaccgcac ccacacatgc cggatgcggg ggccgtcgaa ttcgcacagg
     8341 aagattccct gccacatacc gagttgcagc gtgccgcctt tcacgatcag gtgaagggag
     8401 gtgccgatca gtgcggactt gatgtgggcc gcagcgttgc ctccggtatg cgtgtaatac
     8461 ggacggtccc acggcacgag ttcatccatc tgccgcaaca gatccgcggt cacggtcggg
     8521 tcatcgccct ctgtgaccgt caggccgcag gtcgtgtgcg ggacaaacgc ggtgaccacg
     8581 ccgtccgtga tgccgagaga cttgatggcc tcccggatgg aatccgtgat gttcacgact
     8641 tggctgtgca ggcttgtctg gacatcgaag gttgtcatgt cgcccccctt tccgtagcgc
     8701 cgttatgtgt gacgaaatcg gaataacaga tcactgtctt cctgccattc aacgttagtt
     8761 tatggcgtgg ctgccgggac ggcaaggaaa ttcgtcattt tcgatgcgcg ggcgcggttt
     8821 gggtctgttt tgcgcgtacc ggttcggaca gtcaggccag ttcgctgagc atcatcgcgg
     8881 accaggtcat gacgcgttcg aattgatcta gaccgaagga ttcgttgggg cagtggatct
     8941 tgtcttcctc acgaccgaag ccgaccagaa gcggggccgc gccggaaacc tgccgcagta
     9001 gggcgacaac cgggatggag gcgccctccc agcggaaaag cgcgccgcgg ttgtccagac
     9061 gttccaagac atccgcggcc agccggaaca ggggggagtg gagctccagc cggaagccgg
     9121 gggcaccggg acagatttcg gtgaggctga gcttgaggcc gcgggggcag tgcgcttcga
     9181 gatgactccg cacggcatcg aagacttcgt ccggccgctg gtctggcact aggcgcatgc
     9241 tcaacttggc gaggacggtt gatgggatca cggttttgga acccgggccg ccatagccgc
     9301 cgtggatgcc attgatctcg atcgtgggct cgaaactgcc gcgcgcgacc ggatcgagcc
     9361 gccgatcccc gccgaccggc ggacagccga tctccgcctc gtaattctcg gcgggcgcgg
     9421 cggctcggcg ggcgtgctcc aattcttcgg cttccggcgg tcggacccgg tcccggaatc
     9481 cggcaacggc aatggcaccg tccggagtgt ggagagaggc cagaagccgg gccatgcctt
     9541 gggccggatt gggcgcgacc ccgccatgga tgccggaatg cagatcgtat gccggtccgt
     9601 tcagggtcag ggtgaagtgc tgcacaccgc gcaggcccgc gatgatggcc ggttggttct
     9661 ccggaccgca ggaggtgtcg cacaccatca tcacgtccgc ctgcaggcgc tggcgcaggg
     9721 agggagcgag agcggtgagc ccttcgctcc cgctctcctc ttggccttcg atcacgatgc
     9781 ggagcgttgg taagggcact ccggcttcga tcaatgcttt gaccccctga agaaaaccga
     9841 acacctggcc cttattgtct tgcgcgccac gcgcatacac gcgtccgttg atcagcgtgg
     9901 gctcgaacgg cggggtcagc cacgcttcga gcggatcctc cggctgcaca tcgtaatggc
     9961 cgtagaacag caccgtggac gtgccgttgc cgggccgttc cgccagtacc accggtacgg
    10021 gatgtccgct ttctggagtg atgatgtcca cgctgaagtc cagtttacgg agaaagactt
    10081 tcagccaggc ggccgcccgc gcacactcgc cgagacgctt cgggtcggta cccacagtgg
    10141 gtagacgcaa gagggcgaac cactccttta gaatcgtttc tcggttgcgt tcaaaatact
    10201 caacgggtaa cgagggtcgg atcatcgtgt ctccttctgg tatgaaaaga gaatacccga
    10261 ttggcgcgga ttaggaaacg ccaaaaacaa ttttcacttt tgcacttgat gcgaatcgga
    10321 aaaaagtaaa cactacccat ttctatttgc ccccatcagg ggtagggttg cctgtgaaaa
    10381 aaacctattg cacatccggg ttgtcaggtg tgaccgatcc gcaggcaata gcgagcaagg
    10441 cgacaaggag tgtaagacta tgtccaggac cataccgctg gaccagatcc gcaacatcgg
    10501 gatcatggcg cacatcgacg cagggaagac cacgaccagc gagcgtatcc ttttctattc
    10561 cgggaagaat tacaaactcg gcgaggtgca cgagggtgcc gccaccatgg attatatggt
    10621 gcaggagcag gagcgcggca tcaccatcac ctctgcggcg accgcggttt catggcgcga
    10681 tcaccagatc agcctgattg acacgcccgg acatgtggat ttcactgcgg aagtcgaacg
    10741 gtcgctacgc gtgttggacg gcgcgatcgc gctcttctgc gcagtgggcg gcgtgcagcc
    10801 ccaaaccgaa caggtgtggc ggcagagcga gaaatacgag gtgcccaagc tctgcatggt
    10861 caacaaaatg gaccggatcg gcgccaactt cttcaatgtg gtgaacgaaa tccggtacac
    10921 gctgggcggc aaccccgtgg tgatggttta tccgatgggt gtggcggact cttttcgcgg
    10981 cgtggtcgac ctgctgacga tgaaggctct tgtttttgat gaggaggacc gcggtgtcag
    11041 ggttctggtg gaggaggtgc cggaggagtt ccgcgaggag tgtgagcggt tgcgcgtcga
    11101 aatgatggag caggccgccg aacaggacga tgcgttgatg gccaagtatt tcgagaccgg
    11161 cgagttgagt attgaggaaa tcaaatccgg tttgcggaaa ggcaccatca atcgtaaact
    11221 ggcgcccacg tattgcggca cggcattcaa gaacaagggg gtgcagcccc ttctggacgg
    11281 tgtgatcgac tatctcccgt ctccggctga catcggctct gtgcgttcgg cggacaaccc
    11341 cgaaaatctg cgcagtccgg atgagaacga gccgtttgcc gggttggctt tcaagattct
    11401 gtcggacaag cacatgggca agatcacctt tgtgcgcctc tattcgggca cgttgcgtgt
    11461 gggcgatgag gtcttgaaca gcacgatgga gcagcccggc cgcatcgggc ggctcttccg
    11521 tatgcatgcg gcccgccagg aggccctgga ggcggcgtat gccggcgaca tcgtggctgt
    11581 ggtcggcctc tctaagaccc gcaccggcga cacgatctgc gatcccaagc atccgattct
    11641 cctcgagtcg atcgaattcc cggcgccggt gatctcaatc gcgatccggc ccgcctcgcg
    11701 cggggacaac gagaaactcg gtaacgccct gtactcgctc gcgcaagagg atccgacctt
    11761 tgtggtccgg tatgatcagg aaacgggcga gacgatcatt tccggcatgg gtgagttgca
    11821 ccttgaagtc attgtggacc gcctgcgtcg cgagtacggt gtggcgtgcg aggtcggcaa
    11881 gcccgaggtg gcgtaccgcg aaacgattcg tcatatggtg gacggcgaat ataagcacgt
    11941 caaacagtcc ggtggtcgcg gccagtacgc gcacgtctgc ctccgtctgg aacctcagga
    12001 gccgggacgc ggcttctcct tcgtcaatga ggtcaaaggc ggcgtgatcc cgaacgagta
    12061 tattccggcg gtcgagaagg gcgtcatcaa agcgatggaa aacggtccgt gcggcgggtt
    12121 cccggtggtg gatgtcaagg tcgtggtgca tttcggttcg taccacgcgg tcgactccag
    12181 cgaattcgct tttgtggagt gcgcgcgcca gtgcttcaag aacctcttcc tcaagggcaa
    12241 tccgcaactg ctcgagccga tcatgagtgt cgaggtggcg gtccccgaag attatatggg
    12301 tgccgcgacc gggtcgatct gccagcgccg cggtcgggtc gagggaatgg acgaaaaggc
    12361 cgggagcaag ctgatccgcg ggttggttcc gttgggcgag atgttcggct attcgaattc
    12421 gatccgtacg ctcactcagg ggcgcggtac gttttcgatg acgttcgagc ggtatgaggg
    12481 cgtgcccttc gagctcgccg agaccattat cgaaaagcgc aaaaaagagg gcaaggtgcg
    12541 ttaagcacgg atgcgtattt ttatgcgatc ctgccgccct gcgatgggcg gcttcttttt
    12601 gtaatgaaaa cgggcgcccc gtaaacaaac agggcgccct tggggcaaac aaagcgcaaa
    12661 gaaatcgggg cctacgcttc ggcctcgagc tgctcgacaa gcgcgtcgtc gatatccata
    12721 ttggtgaaga cgtcctgtac gtcgtcattt tcttccagcg cgtcgacaaa cttgttgacc
    12781 gccttggcga ccgccaactc tttgaccggc accaaggttt gcgccacgag cccgaccagt
    12841 gagttttcct gatcgaacgc gatttcggcc gcctccagcg ccgcgcacac ttccgagaac
    12901 gccgcaggcg agcaggtgac ggtgaaaccg tcctcttcgg attccatgtc gtcggccccc
    12961 gcaatgagaa cgacctccat caccttgtcc tcggtcaggc cctctgccgc cggaatcatg
    13021 atcgtaccct tgcgctcgaa accgcgcgaa actgagccca aagttgcaaa atcagaaccg
    13081 ttctttttga agatgttccg gacttcagcc gccgcgcggt tcttgttgtc ggtcagcacc
    13141 ttgactacca gccccacgcc gccctgggcg tagccctcat aggtgatctc ttcaaggacg
    13201 tcggccgcca gctcgcccgt gcccttttta atggcgcggt cgatgttgtc gtttggcatg
    13261 ttgaccgctt tggcctttgt gatcagggta cgcagcgtgg ggttggtgtt ggggtcgccg
    13321 ccgccggctt tggcaacgac cgttagttcc ttggcgattt ttgagaagat cttaccgcgc
    13381 ttggcatccg ccgcgccctt cttgtgctta atggttgacc atttactatg accgctcatc
    13441 gttacccttt cagccgcgca ccatgcggct cgttgactct ctacaacact gtcctacacg
    13501 ggcgtgttcg agcccgcacc cacagcacct tcgcctgcgg gcatattctg ccccagcagg
    13561 tcggcgggcg cttcgccttc aaactcctgt atccagccgt tccccaatcc gaggtcttcg
    13621 gctgcctcca ccagtaccgc gtactcgtca gccgtgacct tgcggtccca gcccggcgtc
    13681 tgcaacgcgc ggtgcaacgg cgtgtattgc gacatcacac tgacgtggat accgctgccc
    13741 gtatgccgcg ccagccattc gaggttctcc accgcttcgg cgatacgccc cggcaacgcc
    13801 aagatccggc agatcacgcc gcgcacagcg attcccgagg cgtcggtctc cagcggcccc
    13861 agacgctccc agaaccaccg caacgcaccg cgcgccgcct cgacgtacgt ccgcgcatcg
    13921 gaaccttccg ccgcgctctc ggcccgggca tagcgcagat caatcaacgc aatgtccgcc
    13981 aagtcagcga acgcctccaa cgtctccagt gactcgtaac ccgacgtatt atacacaaac
    14041 ggaagggcga ttccagtgcg aagtagcggc tccgccgctt cgcggatctg cggcagccac
    14101 ggggtggggg agaccagatt ccagttgtgg cagccctgcc gcgcaagctg ttcgagaata
    14161 tcgcgcagtg cctctaccgt gaagtcgtcg ccatgtcccg cttggctcca cggatggttc
    14221 tggcagtaga tgcagcgcat cgtgcagtgc gagaagaaga tggtgcccga accgcgcgtc
    14281 ccggtgatcg gcggctcctc gccgaaatgt gcgccatagc ggaagacgtg cggcagcacc
    14341 cccgcgcggc agtagccgcg ctggcccgcg aggcgatcca cgccgcatgc acgcgggcag
    14401 agacggcagg aactcagaac gccgaacgcc gaactcagaa cgccgaagtt tttttcgtcc
    14461 tgcgttcccc gttccccatt ccccattctc catccttcgt tctgcgttcg catgcggtca
    14521 actgtagacg gaaggttcgg cgttttcaag agaaagtcga ttcaacctaa aaaaggtgaa
    14581 aacggatttc agggctctgc tcttttttct cacacgaagg taagaagggc acgaaggttt
    14641 ttggggaaca ctgctcgggt ttgtttaatg cagagacgcg gagcgcaaag agtcgggagc
    14701 gcgggcgtct tccgcgctac gcgtggattt aggagttagg agtta
//
LOCUS       DAOSRB010000054        14359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4290_length_14359_cov_7.230914, whole genome shotgun sequence.
ACCESSION   DAOSRB010000054 DAOSRB010000000
VERSION     DAOSRB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14359
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4290_length_14359_cov_7.230914"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLZ74_04525"
     CDS             complement(<1..776)
                     /locus_tag="PLZ74_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR97620.1"
                     /translation="MTSHTAFQEALRTTGRYATVSERHQQNPIRIRALSSFLYAFTII
                     GIAVRCTVRHLFRALDYEEWGRICMASVRFAERLGGIVTFEGFEQRAVYDGPVVYVSN
                     HMSTLETMAFPTTLLSFGKIAIVLKKSLTEIPLVGKAALHIGCIPITRKNAREDLKTV
                     LEEGAKRLAEGHSVLLFPQGTRQAVFDGKKFNSLGSKLAERAGVPVVPLAVKTDFLQT
                     GKWFKDFGTVDPNKPIRFACGPVLPAMLGARATHDQSVAF"
     gene            924..2225
                     /locus_tag="PLZ74_04530"
     CDS             924..2225
                     /locus_tag="PLZ74_04530"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOR97621.1"
                     /translation="MIPQWVIEKKRDGGTLEEAEIRDFIEGFTRGSVPDYQMSALAMA
                     ILFRGMNAAETAVLTDAMMRSGDLVSFEGWPRPTADKHSTGGIGDKLSLMIAPLAASA
                     GLAVPMIAGRGLGITGGTLDKLESIPGYNTCLSVEAFKRVIAEVGCAIIGQTDRLAPA
                     DKKLYALRDVTGTVPSIPLITASIMSKKLAAGAETLVFDVKCGPAAFMRTREDARALA
                     EGLLAVGRALGRRTAALITDMAQPLGRTVGNAVEVAEALETLKGGGPPDTRELTIELT
                     AQMTLLAGVYADPTEARRELAARLDDGRALECFCRMVRAQGGDARVADDPRRLPQPGA
                     VREIPAPQDGIVATVDAEKLGRVVLRLGGGRTVTTDEIDPAAGIDRLVQTGERVEKGQ
                     PLMRLLAKNAACFDPLAAEAAAAVRITGEPVAERKLILENL"
     gene            2236..2697
                     /locus_tag="PLZ74_04535"
     CDS             2236..2697
                     /locus_tag="PLZ74_04535"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOR97622.1"
                     /translation="MSDPSDRNVAMKKEKLPEVPKSLRDSLVKAAREAAVKAYVPYSD
                     YPVGAALLTPDGQIYTGCNVENASYGLSVCAERVALFKAVSEGCRSFTALAVAGGQRR
                     AAVPCGACRQVLAEFCAPGMPVFSARLKGGTVTSATLAELLPLAFTGSDLG"
     gene            2919..6878
                     /locus_tag="PLZ74_04540"
     CDS             2919..6878
                     /locus_tag="PLZ74_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97623.1"
                     /translation="MKTKAVKRSSAGNVVSVRSVMRAAAMLCAIAVSLCADDTMITTG
                     DYYGTLWGDGRLWTSGSAPTGAGITLDTGSGSPQIRMDETNRMAGRITSTFGTMPAEG
                     GQFLFSVVNNGFRHTDTLAPQGIFGNNFHLTTNTFTFDNNGNDAQIRIEDGTGGNGGT
                     ARKPYRYLFDVPVVLADNLAIRHLFTGTNQYGSYTTSQGDGPAAGVIFSRKISGGGKG
                     ITIENTAATLGVIFMGDSEFTGDLTVNRGLARAKDTSNLPRVGTPFGQANSVIATNDE
                     AGVDLGGFITGAGQTLRLKGKRTCLGGTNGVLFSTWARPGDLSEWQGNVILEGETWIG
                     GTLTGNNWPANFYPIDTDGGLAISGPISEAGGSATLHVLNGRDTVFSGANTFSGGLYL
                     DKGFFTARGAEAFGTGGIYFNGGIYRVTSAEDADIFTADVHGAAGGFRVFLDAGVTCT
                     MASNAVSGLVKPAFYKYGPGTLVLTNRVSTGTSLCIMGGTVVLDTTREGNVKFGPNNN
                     NWEMDHVYLYNDATFVVRGDPATVRTGAWYGTPQAGLSGTFRAEDGQRFNLAFNADNN
                     LLGCVNLETDNSGAYFTGAGSAGSHTYNSDYAFLVYEGSSWVYTSGGRAVPLPDSAYQ
                     TTWGGKTDIVDVTPALAAVPVPASTEADVIRFNTPNGGAEIVLTLAGDLTLDGRTILV
                     TPAMGNTPVRITGGAIKSSGLIRILNFNTNATLTIESDLAKNVANTQLITGGPGKTFL
                     KGAKTFNGLVYVVGGELEVDCADALGTANAAWNSGYVFLSNGGVLSFNGVINPVLSAT
                     TRTEFSLRVSSTGGGLKVPDADDVLTLSENGINVCGGGLFRKTGAGTLELKNSAKMST
                     QDEGGYKPNFARTDFAEGTVIAATGFQEGAETMTVRNGVSLKGPRWIAPSQAGANGGG
                     NRMELQAKRQLVAGAGGATVDLQGQNVSLADSRSFYGLDKSTEFFGGTGEIRVVNTSA
                     TEAKIQCYGYHDAAFRGKFTSHVPVTGEQGLGFRNAEWCVPEGVTNTFTQIAEDRFML
                     WFGRLSGKGTLTAQAIQTYISGRFFLGQDDAPAADFEGTLGVYCGGNSIGALQLVKVG
                     NNAQRISGAANTFEGHTIIRAGSLLVGNNSPATAGASGALGQAMVYVGDAETPAGASP
                     ALLADGAYTIANEIRVHDFSPASATPVLGGTANADGALFSGAVRLYRDVIFRAETGTT
                     VTFTGEIEGLKGAGMSGGGRVVLADDVTLGGGFVWTGGTLQVNGAFTIPEGAALTVDA
                     SVCTKENRGTIFTLIRANGGIFGTFMFAQELPAGWRVATRAGAVCLLFDAGTTLFLK"
     gene            6907..8790
                     /locus_tag="PLZ74_04545"
     CDS             6907..8790
                     /locus_tag="PLZ74_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97624.1"
                     /translation="MKRHTHRCWGSVLAAGFTAFAVQAVDITFPNTDGKGDLASTASW
                     NQTPIPDPTNRPEFKATLMGATLTASEDISFAGFKINVNNTAYPVILDMRNEATGANP
                     GPRKITLSDNIRNVSGNNITYIKGGEWTGTSISTDWGKSTDIYYVQGAVMTLSGGLAG
                     TYGGNGALIELSGGSVVTALHARAQYNYGDNSSIQVLDGSKLVVTGVADESFMSDKSS
                     GFNNGYVVRGENSLIKKTSSAKAALLGAGGHSAYLRVEDRGVADFTGTLRVGNAANST
                     NNVVSVTGGGTLKADGVQLGYSSSAGNNLFAITNGGVAEVPNGFFVGGNAYEGCFGNT
                     LLVSNGVFRGKVPTIGFKAGSSNNLVRIVGRQSVYEAVANVSSDTACIFAYERNNRFE
                     LDDAVWTNNVAKITYLNCSQFTEGHCVMELRNNAEFYTTNQFYMTGIHYRSLSNLLSI
                     VSGSRLYSKGSVGVYSIGNVFSISNGTLETEGSFTVTAQSGSPMMYGYSNNWVRVAGE
                     RPRISVTNGTFAVQYASYLQFDIPASGYEAGAAPVKAKAFTVSSDSSLIVNGLDARIK
                     AIKEPEVVALVESLNGVTVPAGVLATANAGLPKGCALFVSADNKALLLRVYKETGTLL
                     SIH"
     gene            8803..11208
                     /locus_tag="PLZ74_04550"
     CDS             8803..11208
                     /locus_tag="PLZ74_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97625.1"
                     /translation="MMSLFALPVLAAALSAVTSNPSNNFFVAGHEAKATLTAKGLAAG
                     EKRPVRLEVYDFTYTNRVATVPGEIVADEKGEWRGEFTLPSDRYGVYLVRPVAAGLEL
                     PKRGTVPKGAFTYAVLADPSEMPEIDLWDTFLGLHGGSYSWMYQRGGLGDARKPSEDR
                     FIVANLDLFNREKDFWKSFLTNEAVQAKYRADVTGYVKAAVAAGGGKQKRRFYEPTWE
                     ANLKTPNAETLVKVHQIAWEVIHELDPDAYVGAPTSAGINDLAFLRSLFDLGLAKYMN
                     AFAVHPYIAAPPEANGLVDTVRSLKRMLREYMGRDVPMIGTESGLNEPNSPLGEKTKL
                     TGQLRQNLILFGEGFLWNLPFYGYDFGADANNQDEGDYGVCYNPLYPKHRWGPGEVQP
                     RPVFGALAAFGRLTEGHRPTCPIEWLGETVLGYAFADKSDSHCVIALWDWGERGTEVE
                     LPVGRDAIDVADVMGNVTRKKTTGGTLKLTLSEYPQYVLDANSAIWGKAAQAKLKWGE
                     RKYMSDSDRAPVGIADLAPSFASGGPGVAVTLENRTDEPHRVALETRIPGEPDARVKR
                     SVSLAARQTQRIEIAFDGFTPDPTVFFDVEVRVVPEKGMVATAVDSFNFLPVPSSFAF
                     DGGKTVSLGYDARFLTFDIFIPDSTPTNGWTGWDSWKGDSVQVGLAKSALKKRTQNDL
                     ADNAAEAKSEITFALTPNGPEMYRTITWDVRRFPCDTHLAGQIDLKEAPLTVEKSAKG
                     FRYRASVPWAFLNLSAPKPGDTFRFAFQFNDREPGNRFLKESKGFELKQAAPKKFGWG
                     TIR"
     gene            11205..12518
                     /locus_tag="PLZ74_04555"
     CDS             11205..12518
                     /locus_tag="PLZ74_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97626.1"
                     /translation="MTALLLAASLQFHGVMGQSAAKGEAPLEFTGVSFLSEGPCGRVF
                     FGGDDGKLYELKDGEIIRTGVRMQGRFLDWDGKTVRALGQHDVWEVDPATLAFKHVIR
                     TRDFRFDLAAVVPDDPVHPFTAKGKYVTYDKKRDALLVLDEKGAERGGLFPLPERLSK
                     SEIISLGFLPDSGDLVAVSYYPDLRIYRFTAEGTQVTGHGWPARRGFGFFRKSGGELY
                     HCGTPTLVRMEENLAGAQGNVFRVGKEHFTCGYAKQGDREYIGTSQGLYVKEPGETEF
                     RKRLGGIRPLTALAVNGKYVYMSMGGTIRWMYLDEDENGPFASSDDHIFRVNGGWTEK
                     PYDLIPDGDNLLVAAGKLGTWRFKPFLPPAYAFNRKHWEQVSTNVCTELSVGKPSCEL
                     LGFLSSAEVPDGLEPGKIASQGKWLVVEDRKNFRLVRFRIDADKK"
     gene            12631..>14359
                     /locus_tag="PLZ74_04560"
     CDS             12631..>14359
                     /locus_tag="PLZ74_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HOR97627.1"
                     /translation="MRTQTVAVGILAGILWSAGLRAQEVLGPMGGVGGEARRVEQQAL
                     TPVVPSVTRKSEAAPIPAPQAPAVPGGKDDGPAWVIRAITLKGDLSFAEKRGVKGALE
                     AALAGETARSRQEVQEALRKVFNDFIRDGYYLARVSLPRDPYDAKEKTLTVLVEEGLF
                     GQVRIGFADDREDGRWFSREQIARRFRDIRPGDTFNYKTLYDCLYDANAHPDLTLDTK
                     IAVRKPLEGEGDARRVVRYADIDLTVKESLPFHAVFDINNYAIEAIDEWQASLTLQYL
                     NLTKADDVLTVSPSMSLDSSLMSLAGSYMRPHDIWKGGATTVYAGWSELDCDDVVPFI
                     DLEGTGWFVGLVHSHTLVEDEDRLISLSGGIVYRYIEDQFSAFNTSLQQRDVSVLPLS
                     LALSYSARRADAFRGRNFATLQGVYNLAAGGGNELEDMWVDAEDDYLIGRLQLARLQP
                     LFGTLDNMQRQVHQWILFFKAEGQYASCPLIPAEKLALGGFNTVRGYQTKGYLGDNGL
                     YGTVELRTPILLDMVSHTLGRKPGSTPLDRLQFVAFTDFGFIEFIDPLPGVDDNETLL
                     SVGAGVRLAV"
BASE COUNT         2764 a         4134 c         4815 g         2646 t
ORIGIN      
        1 atgaacgcga cgctctgatc atgcgtggcg cgggcaccga gcatggcggg gaggaccggg
       61 ccgcaggcga agcggatcgg cttgttgggg tcgaccgtgc cgaagtcctt gaaccacttg
      121 ccggtttgga ggaaatcggt cttcaccgcc agcggcacga cgggcacacc cgcgcgttcg
      181 gccagctttg aacccagcga gttgaacttc ttgccgtcga aaaccgcctg gcgcgtgcct
      241 tgcgggaaga gcagcaccga atggccttcg gccaggcgtt tcgcgccctc ctccagtacg
      301 gttttcaggt cttcgcgcgc gtttttgcgc gttatcggaa tgcagccgat gtgcagagcg
      361 gccttgccga ccagcggaat ctcggtcagg ctctttttaa gcacgatcgc gattttgccg
      421 aacgagagca gcgtggtcgg gaaggccatg gtttccagcg tgctcatgtg gttggacacg
      481 tacacgaccg gcccgtcata tacggcgcgc tgttcgaagc cctcgaaggt aacaatcccg
      541 ccgagccgtt cggcgaagcg taccgaagcc atgcagatcc gcccccactc ctcgtagtcc
      601 aaggcacgga agaggtgtct caccgtgcaa cggaccgcaa tgccgataat ggtgaaggcg
      661 tagaggaaag aagagagcgc acggatccgg atcgggttct gctgatgccg ctcggagacg
      721 gtggcgtaac gtccggtagt gcgcagggct tcctgaaacg cggtatgtga ggtcatggtg
      781 cgggacacta taccaaaaac ttcggggcgc ggggacggaa aaaccgcagt tgtgcgggcc
      841 cggcttttgg ggtatacttt cccccgaata atggggattg aaagagcggt ttggaaagac
      901 cggtggacgg tgaaggaggg gacatgattc ctcaatgggt gattgagaaa aaacgggatg
      961 gcggaacgct tgaagaggcg gagatacgcg atttcataga ggggttcacg cgcgggtcgg
     1021 tgccggacta ccagatgtcg gctctggcta tggccatcct attccggggg atgaacgctg
     1081 cggaaacggc ggtgttgacc gacgcgatga tgcgttcggg cgacctcgtg tcctttgagg
     1141 gatggccccg tcccacggcg gacaagcact cgaccggcgg tatcggagac aagctctccc
     1201 tcatgatcgc gccgctggcg gcttcggcgg ggctggcggt tccgatgatc gccgggcgcg
     1261 gcctggggat caccggcggc acactggaca agttggagag cattccggga tataacacat
     1321 gcctttcggt ggaggccttc aagcgggtga tcgcggaggt cgggtgcgcg atcatcgggc
     1381 agaccgaccg tttggccccc gccgacaaaa agctctacgc gctgcgcgac gtcaccggca
     1441 cggtgcccag catcccgctg atcaccgcca gcatcatgtc caaaaaattg gccgcgggtg
     1501 ccgagacgct ggtcttcgac gtcaaatgcg gtcccgcggc ctttatgcgc acacgggagg
     1561 acgcgcgtgc gctggccgaa ggcctgctcg ccgtgggccg ggcgttgggt cggcggaccg
     1621 cagcgctcat tacggatatg gcgcagccgc tcgggcgcac cgtgggtaac gctgtggagg
     1681 tggccgaggc gctggagacg ttgaagggcg gcgggccgcc ggatacgcgc gagctgacga
     1741 tcgaactcac cgcgcagatg actctgctgg ccggggtgta cgcggacccg acggaggcgc
     1801 ggcgcgaact tgcggcgcgg ctcgacgacg gccgtgccct ggagtgtttc tgccgcatgg
     1861 tgcgcgcgca gggcggcgac gcgcgtgtgg cggacgatcc gcgtcgcctg ccgcagcccg
     1921 gcgcggtacg ggagattccc gcgccgcagg acgggatcgt ggccaccgtg gatgccgaaa
     1981 agctgggccg ggtggtgttg cggctcggcg gcggacgcac ggtcacgacg gacgagatcg
     2041 atcccgcagc cgggatcgac cgtttggtcc agaccggcga acgggtggaa aaaggccagc
     2101 cgctcatgcg gttacttgca aaaaatgccg catgctttga tccgttggcg gcagaggccg
     2161 cggctgccgt gcgcatcacg ggcgaaccgg tggcggagag gaagctgatt ttagaaaatc
     2221 tctgacgggt ctgacttgtc cgacccgtcc gacaggaacg ttgcgatgaa aaaagaaaaa
     2281 ctccctgagg tccccaagtc attgcgcgac agcttggtga aggccgcgcg cgaggccgcg
     2341 gtcaaggcct acgtgcccta ttccgattat ccggtgggag cggcgcttct cacgccggac
     2401 ggccagatct atacgggctg caacgtcgag aacgcctcgt atggcctgag cgtgtgcgcg
     2461 gagcgggtcg cgctgttcaa ggcggtcagc gagggatgcc gcagtttcac ggccttggcg
     2521 gtggcgggcg gacagcggcg cgcggcggtt ccctgcggtg catgccgtca ggtgctggca
     2581 gagttttgtg cgcccgggat gccggttttc agtgcgcggc tcaagggcgg cacggtaacg
     2641 tcggccacgc tggcggaact gttgccgctg gccttcacgg ggtcggatct gggctaatag
     2701 aataacgcaa accgatttga tcccgtgcga aagcggtgcc gtctgtcggt tgttcccgca
     2761 ctccacattt catgcggtat tgcgccctgc gcgtagtccg gtgcgttttc gatattgacc
     2821 ccttctgtcc cccccctgca taataaagag gtaactgtgg aaaatgaaaa ggtaccgggg
     2881 atgctcgtcg tgttgtttcg tgatcaggag acggtgaaat gaaaacaaaa gcagtaaagc
     2941 gtagttcggc tgggaatgtc gtgtctgtga gatcggtgat gcgagcggcg gcgatgctct
     3001 gcgcaattgc ggtcagcctg tgcgcggacg acacgatgat cacgacaggt gattactacg
     3061 gtacgctatg gggtgacgga cggctctgga cgagcggcag cgcgccgacc ggcgcgggca
     3121 tcacgcttga tacgggcagc ggcagtccgc agatccgcat ggacgaaacg aaccgcatgg
     3181 caggaaggat cacctcaacg ttcggcacca tgcctgcgga aggcgggcag ttcctcttct
     3241 cggtcgtcaa caacggattc cggcatacgg acacgcttgc gccgcagggc atattcggaa
     3301 acaacttcca cctcaccacc aacaccttca cgttcgacaa caacgggaac gacgcgcaga
     3361 tccgcatcga ggacggcaca ggcggaaacg gcggcacggc gcgaaaaccg taccgttacc
     3421 tctttgacgt tcccgttgtc cttgccgaca atctcgcgat ccgtcatctt ttcacgggca
     3481 cgaaccaata tgggagttac acaaccagtc agggcgacgg tccggcggcc ggggtgatct
     3541 tctcccggaa aatctccggc gggggaaagg gcatcaccat cgagaatact gcggccacgt
     3601 tgggggtgat cttcatgggg gacagcgagt tcacggggga tctcacggta aaccgcggtc
     3661 tcgcccgcgc caaagacacc tcgaatctgc cgcgggtggg cacgcctttc ggccaggcga
     3721 acagcgtcat cgcgacaaac gatgaggcgg gtgtggatct tggcggcttc atcacgggtg
     3781 cggggcagac cctgcgcctg aaaggcaagc gcacctgttt gggggggaca aacggggtgc
     3841 tcttctccac ttgggcgcgc ccgggagacc tctccgaatg gcagggcaac gtgatcctcg
     3901 aaggggagac ctggatcggc ggcacgttga ccgggaacaa ctggcccgcc aatttctatc
     3961 cgatcgacac ggacggcggt ctcgcgatct cgggcccgat ttcagaagcg ggcgggtcgg
     4021 cgacgctcca tgtgttgaac ggacgggaca cggtctttag cggcgccaat acattcagcg
     4081 gtggacttta ccttgataag gggttcttca cggcacgcgg cgcggaggcg ttcggcaccg
     4141 gcggcatcta cttcaacggc ggcatctacc gcgtcacctc tgctgaggac gccgacattt
     4201 tcacggccga cgtgcatggc gcggcgggcg ggttccgcgt ttttctggac gcgggcgtga
     4261 catgcaccat ggcctccaac gcggtcagcg gccttgtcaa accggctttc tataaatacg
     4321 gtcccggcac gcttgtgctc accaaccggg tcagcacggg gacttcgctt tgtatcatgg
     4381 gcggcacggt tgtgctcgac acgacccgtg aagggaacgt gaagttcggt ccaaacaata
     4441 acaactggga gatggatcac gtctatcttt acaacgacgc aacctttgtt gtgcggggtg
     4501 accctgcgac cgtgcggacc ggcgcttggt acgggacgcc gcaggcgggc ctctccggaa
     4561 cgttccgcgc ggaagacgga cagcgtttca acctcgcttt caacgccgac aataacctgc
     4621 ttggctgcgt caacctcgaa accgacaaca gcggggccta ctttacgggt gcgggttcgg
     4681 ctggcagcca cacgtacaac agcgactacg cattcctcgt gtacgagggc tcatcatggg
     4741 tctacacgag cggcggacgc gccgtgccgc tgccggacag cgcgtatcag acgacgtggg
     4801 gcggcaagac cgacatcgtt gacgtgacgc cggcgctcgc cgcagtgccc gtgccggcca
     4861 gtacggaggc ggacgtgatc cgcttcaaca cgccgaacgg cggagccgag atcgtgctca
     4921 cgcttgcggg cgatctcacg ctcgatggcc gcacgatcct tgtgacgccc gcgatgggga
     4981 acacgcccgt gcggatcacg ggcggcgcga tcaagagttc cggcctcatc cgcatcctca
     5041 acttcaacac gaacgcgacc ctgacgatcg aaagcgacct cgcgaagaac gttgccaaca
     5101 cacaactcat cacgggcggt cccggcaaga ccttcctcaa gggcgcgaag accttcaacg
     5161 gtctggtcta cgtggtgggc ggcgagttgg aggtggactg tgcggacgcg ctcggcacgg
     5221 cgaacgcggc gtggaacagc gggtacgtgt ttctttcaaa cggcggcgtc ctttcattca
     5281 acggcgtaat caatccggtc ttgagcgcga ccacgcgcac agagttcagc ctccgggttt
     5341 cgtcgaccgg cggcggcctc aaggttccgg acgcggacga cgtgctcacc ctttcagaaa
     5401 acggaatcaa cgtctgcggc ggcggcctct tccgaaaaac gggcgcaggc accctcgagt
     5461 tgaagaatag cgctaaaatg tcgactcaag acgaaggagg ttacaaaccc aacttcgcgc
     5521 ggaccgattt cgcggaggga acggttatcg ccgctacggg cttccaagag ggagcggaga
     5581 cgatgaccgt caggaacggg gtcagtctga agggcccgcg gtggatcgcg ccgagtcagg
     5641 ctggagcgaa cggcggcggc aaccgcatgg aactgcaagc caagcgccag ctcgtggcgg
     5701 gtgctggcgg ggcgaccgtg gatctgcagg ggcaaaacgt gtcgctcgcc gacagcagaa
     5761 gtttttacgg tctcgacaag agcaccgagt tctttggcgg cacgggcgag atccgggtgg
     5821 tcaacacgag cgcgacggaa gccaagattc agtgctatgg ctaccacgac gccgccttcc
     5881 gcggcaagtt cacgtcccat gtgccggtca cgggtgagca gggtctgggg tttcgcaacg
     5941 ccgaatggtg tgtgccggaa ggcgtgacca acacgttcac ccagattgcc gaggaccgtt
     6001 tcatgttgtg gttcggacgc ctttccggaa agggaacgct caccgcgcag gcgattcaga
     6061 cttacatttc ggggcggttt ttcctgggtc aggatgatgc gccggcagcc gattttgagg
     6121 gtacgctggg cgtgtactgt ggagggaatt cgatcggtgc gctccagctg gtgaaggtgg
     6181 ggaataacgc gcagcgcatt tcgggcgcgg cgaatacgtt cgagggccat acaatcatcc
     6241 gcgcgggatc gcttcttgtg gggaacaact cgcccgcgac ggccggcgct tccggcgcac
     6301 tgggtcaggc gatggtgtat gtgggcgatg cggagacgcc ggcaggggct tcgccagcgc
     6361 ttctggcgga cggcgcgtac acgatcgcga acgaaatccg cgtgcacgat ttttcgcccg
     6421 cgtcggcgac gccggtgctc ggcggcacgg cgaacgcgga cggcgccctg ttctcggggg
     6481 cggtgaggct ctatcgcgat gtcatcttcc gagccgagac agggacgacc gtgacgttta
     6541 cgggagagat cgaggggctg aagggcgccg ggatgtcggg cgggggccgt gtggtcttgg
     6601 cggatgacgt cacgctcggc ggcggattcg tctggacggg cggcacgctt caggtaaacg
     6661 gtgccttcac gattcccgag ggcgcggcgc tcacggtgga cgcttccgtc tgcacaaagg
     6721 agaaccgcgg cacgatcttc acgctgatcc gggcgaatgg cggtatcttc ggaacgttca
     6781 tgttcgcgca ggaacttccc gccggttggc gcgtcgcaac ccgcgcgggt gcggtgtgcc
     6841 tgctttttga cgccggaaca acactctttt tgaagtaacc cgaaaaagaa caagggaggg
     6901 atgaccatga agagacacac gcatcgctgc tggggatccg tgctggccgc gggatttacg
     6961 gcatttgccg ttcaggctgt tgacatcacg tttcccaata ccgacggcaa gggcgacctc
     7021 gcgtcgaccg cgtcgtggaa ccagaccccg attcccgacc cgaccaatcg gccggaattc
     7081 aaggccacgc tcatgggggc cacgctcacg gctagcgaag acatcagctt cgcagggttc
     7141 aaaatcaatg tgaacaatac cgcgtatccg gtgattttgg acatgcgcaa cgaggcgacg
     7201 ggcgcgaatc ccggtcccag aaagatcacg ctgtcggaca acattcggaa cgtgagcggc
     7261 aacaacatca cttacatcaa gggcggcgaa tggaccggca cgtcgatttc cacggattgg
     7321 gggaaatcca ctgacatcta ttatgtccaa ggtgcggtca tgacactttc cgggggcctt
     7381 gccggcacgt acggcgggaa tggtgccctg atcgaactct ccggcggttc ggtggtgacc
     7441 gcgctgcacg cgcgtgcgca atacaattac ggggacaact ccagcattca ggtgctcgac
     7501 ggttcgaagc ttgttgtcac cggggtggcg gatgagtcgt tcatgtccga caagtcttcg
     7561 ggcttcaaca acggctacgt cgtgcgcggc gagaattcac tgatcaaaaa aacctcttca
     7621 gcgaaagccg cattgcttgg tgccggcgga cactcggcct atctgcgggt ggaggatcgc
     7681 ggcgtggcgg actttaccgg gacgctgaga gtgggcaatg cggcaaattc gacgaacaac
     7741 gtcgtgtcgg tcacgggcgg gggcacactc aaggcggatg gggtccaatt gggatattcg
     7801 agcagcgcag ggaataacct tttcgcaatc acgaacggcg gtgtggcgga ggttccgaac
     7861 ggtttctttg tcgggggtaa cgcctatgaa gggtgtttcg ggaacaccct tctggtgtca
     7921 aacggggttt tccgcggaaa agtgccgacg atcggtttca aggcgggctc ctccaataac
     7981 cttgtgcgca ttgtgggacg gcagagcgtg tacgaggcgg ttgccaacgt atcttcggat
     8041 acggcgtgca ttttcgcata cgaaaggaac aaccgcttcg aactcgatga tgcggtctgg
     8101 acgaacaatg tcgcgaagat cacgtatctg aattgctccc agttcacgga aggccactgc
     8161 gtaatggaac tcaggaacaa cgccgaattc tacacgacca accagttcta catgaccggc
     8221 atccactacc gcagcctgtc gaacctcctg tcgatcgtgt ccggttcgcg gctctactca
     8281 aaggggagcg tcggcgtgta ttcgatcggg aacgtgtttt cgatttcaaa cggcacgctg
     8341 gagaccgaag ggagcttcac cgttacggcg cagagcggtt cccccatgat gtacggatat
     8401 tcgaacaact gggtgagggt cgcgggcgaa cggccgcgga tttccgtcac gaacggcacg
     8461 ttcgctgttc agtacgcttc ctatctacag tttgacattc cggcgtccgg ctacgaggcg
     8521 ggtgccgcgc ccgtgaaggc aaaggctttc acggtgtcgt ccgattcgtc gctgatcgtg
     8581 aacggcctcg acgcgcgcat caaagccata aaagagccgg aggttgtggc gctcgtcgag
     8641 tccctcaatg gcgtgacggt tcctgcggga gttctggcga cagcgaatgc gggcttgccg
     8701 aagggatgcg cgcttttcgt ctcggccgac aacaaggcac tcttgttgag ggtctataaa
     8761 gagacgggga ctttgttgag cattcactaa tcggagtacc gcatgatgtc attattcgca
     8821 cttccggttc ttgccgcggc gttgagcgcc gtgacatcga acccctccaa caattttttt
     8881 gtcgccgggc atgaggcgaa ggcgacgctg acggcgaagg ggcttgccgc cggcgagaag
     8941 cggccggtcc ggttggaggt ttacgatttc acctacacga accgtgtggc gacggtgccg
     9001 ggggaaatcg tggcggacga gaaaggcgaa tggaggggcg agttcaccct tccgtccgac
     9061 cggtatggcg tgtatctcgt ccggcctgtc gccgccgggc tcgaactccc gaaacggggg
     9121 accgtgccga aaggcgcgtt cacatacgcg gttctcgcgg atccgtccga gatgccggag
     9181 atcgacctgt gggacacgtt cttaggactg cacggcggca gttattcgtg gatgtaccag
     9241 cgcggcgggt tgggcgacgc gcggaaaccg tctgaggatc gcttcatcgt ggcgaacctc
     9301 gacctgttta acagagagaa agatttctgg aagagcttcc tcacgaacga agctgtgcag
     9361 gcaaaatacc gtgcggacgt gaccggatac gtgaaggcgg ctgtggccgc gggcggcgga
     9421 aagcagaagc gccgtttcta cgagccgaca tgggaggcga atctcaagac tccgaacgcg
     9481 gagacgctgg tgaaagtgca ccagatcgcg tgggaggtga ttcacgaact cgacccggac
     9541 gcttacgtgg gcgcgccgac ttcggcgggg atcaacgacc ttgcgttctt gcggtcgctc
     9601 ttcgatctgg ggctcgccaa gtacatgaac gcgttcgcgg tgcatcctta tatcgcggcg
     9661 ccgccggagg cgaacgggct cgtggatacg gtccgttcgc tcaagcgcat gctccgcgag
     9721 tacatggggc gggacgttcc gatgatcggc acggagagcg ggctcaatga gcccaactcg
     9781 ccgctcggcg aaaagacgaa actcacgggg cagctccgtc agaacctcat ccttttcggc
     9841 gagggttttc tctggaacct gccgttctac ggttacgatt tcggcgcgga tgcgaacaat
     9901 caggatgagg gcgactacgg tgtatgctac aacccgctct atccgaagca caggtggggg
     9961 ccgggggaag tgcagccgcg gcctgtcttc ggcgcgcttg cggcgttcgg gcgtctgacg
    10021 gaagggcatc gtccgacatg tccgatcgaa tggctgggcg agaccgtgct cgggtacgcc
    10081 tttgcggaca aaagcgattc gcactgcgtg atcgcgctct gggactgggg cgaacgcgga
    10141 acagaggtcg agcttcctgt cgggcgcgac gcaatcgatg tggcggacgt gatgggcaac
    10201 gtgacgcgga agaagacgac cggcggtacc ctgaaactca ccctttccga atatccgcaa
    10261 tatgttctgg atgcgaattc cgcaatctgg ggcaaggcgg cgcaggcgaa actcaaatgg
    10321 ggcgaacgga aatacatgtc ggattcggac cgcgcccccg tggggattgc cgacctcgcg
    10381 ccgtcattcg cgtccggcgg ccccggcgtg gccgtcacgc tcgagaaccg cacggacgaa
    10441 ccgcataggg tcgcgctgga aacgcggatt ccgggcgaac ccgacgcgcg ggtgaagcgg
    10501 agcgtttcac tcgcggcgcg gcagacgcaa cgcatcgaaa tcgcattcga cggtttcacg
    10561 cctgatccga cagtgttctt tgacgtcgag gtgcgcgtcg tgccggagaa ggggatggtg
    10621 gcgacggcgg tggactcgtt caacttcctt cccgtcccct cttcgttcgc gtttgacggc
    10681 ggcaagacgg tgtcgctcgg ctatgacgcg cggttcctga cgttcgacat cttcatcccc
    10741 gactcgacgc ccacaaacgg ctggacgggg tgggacagtt ggaagggcga cagcgtgcag
    10801 gtggggctcg cgaaaagcgc gctcaaaaaa cgcacgcaga acgatctcgc ggacaatgcg
    10861 gccgaggcga aaagcgaaat cacgttcgcc ctgacaccca acggccccga aatgtatcgc
    10921 accatcacgt gggatgtgag gcgcttcccg tgcgacaccc atctcgcggg ccagatcgac
    10981 ttgaaggaag ccccgttgac ggtggagaag tccgcgaagg ggttccggta ccgcgcgtcc
    11041 gtcccgtggg cattcctcaa tctgtccgca ccgaagccgg gcgacacgtt ccggttcgcg
    11101 ttccaattca acgatcggga acccggcaat cgttttctga aggaatcgaa ggggttcgaa
    11161 ctcaaacagg ccgcgccgaa aaagttcggc tgggggacga tccgatgacg gctctgctgt
    11221 tggcggcgtc gcttcagttc cacggggtca tggggcagag tgcggcgaaa ggggaagcgc
    11281 cgcttgaatt caccggtgtc tcctttctgt cggagggccc ctgcggacgg gtgtttttcg
    11341 gaggcgacga cgggaaactg tacgagttga aggacggaga gatcatccgg acaggcgtgc
    11401 ggatgcaggg ccgctttctc gactgggacg gcaagactgt tcgtgcgctc ggtcagcacg
    11461 atgtctggga ggtcgacccg gcgacactcg cgttcaaaca cgtgatcagg acgcgggact
    11521 tccgtttcga ccttgcggcc gtggtgccgg acgatcccgt gcacccgttc accgcgaagg
    11581 gcaaatacgt cacgtacgac aaaaaacgcg acgcgcttct cgtgttggac gagaaaggtg
    11641 ccgaacgcgg tgggctcttc ccgctccccg aacgactttc gaagagtgaa atcatcagcc
    11701 tcgggttctt gcccgattcc ggcgatctcg ttgccgtgtc gtattatccc gatttgcgta
    11761 tctatcggtt cacggcggaa ggcacgcagg tgacgggaca cggttggccg gcgcggcgcg
    11821 ggttcgggtt cttccggaaa tccggcggcg aactctatca ctgcggcacg ccgacgctcg
    11881 tgcggatgga ggaaaacctc gcgggtgcac aagggaacgt cttccgtgtc gggaaggaac
    11941 atttcacgtg cggttatgcg aaacaagggg accgggagta catcgggacg agccagggac
    12001 tctatgtgaa agagccggga gagacggaat tccggaaacg gttgggcggc atacgcccgt
    12061 tgacggctct cgcggtgaat ggcaagtacg tgtacatgtc gatgggcgga acgatccggt
    12121 ggatgtactt agacgaggac gaaaacgggc cgttcgcctc ttccgatgac cacatcttcc
    12181 gggtgaacgg cggctggacg gaaaagccgt acgatttgat cccggacggg gacaacctgc
    12241 tcgttgccgc cggcaaactc ggaacatggc gcttcaagcc ttttctgccg ccggcgtatg
    12301 cctttaaccg gaagcattgg gaacaggtct ctacgaatgt ctgcacggaa ctttccgtcg
    12361 ggaaaccttc gtgcgagcta ctggggttcc tttcttctgc agaggttccg gacggcctgg
    12421 aacccggcaa gatcgcaagt cagggaaagt ggcttgtcgt tgaggatcgg aagaacttcc
    12481 gtctggtgag gttcagaatt gacgctgaca agaagtaacg aagcgacttt gatgttgtcg
    12541 atcacttcaa gtgatcctgt ttttatcgca ttctgccggt gttttcttta ttttcttctt
    12601 tcaacgggtt caggaagggg acggcagacc atgcggacac agacggttgc ggtcgggatt
    12661 ctggcgggaa ttctgtggtc agcgggattg cgtgcccaag aggtgctggg gccaatgggg
    12721 ggtgtgggcg gcgaggcccg cagggttgag cagcaggccc tgacgccggt tgtgccgtcc
    12781 gtgacccgta agtcggaggc cgccccgatt cccgcgccgc aggcgccggc cgtcccgggc
    12841 ggaaaggacg acggcccggc ttgggtgatc cgggcgatca cgctgaaagg cgacctgtcc
    12901 tttgccgaga aaagaggcgt gaagggcgcg ctcgaggcgg cgttggcggg cgagaccgcg
    12961 cgcagccggc aggaggtgca ggaggcgctg cgcaaggtgt tcaacgactt catccgcgac
    13021 ggctattatc tggcgcgcgt ctcgctgccg cgcgatccgt atgacgcgaa agagaagacg
    13081 ctgaccgtgc tggtcgagga gggcctgttc ggtcaggtgc ggatcgggtt cgccgatgac
    13141 cgcgaggacg gccgctggtt cagccgcgag cagatcgccc gccgtttcag ggacatccgg
    13201 cccggcgaca ccttcaacta caagacgctc tacgactgcc tgtacgacgc caatgcgcac
    13261 cccgacctga cgctggacac caagatcgcc gtgcgcaagc cgttggaggg cgagggggac
    13321 gcgcgccgcg tggtgcgcta cgcggacatc gacctgacgg ttaaggagag cctgccgttc
    13381 cacgcggtct ttgacatcaa caactacgcg atcgaggcga tcgacgagtg gcaggcctcg
    13441 ctgacactgc agtacctgaa cctgaccaag gcggacgatg tgctgaccgt cagcccttcc
    13501 atgtcgctgg actcctcgct catgtcgctg gcgggcagct acatgcggcc gcacgacatc
    13561 tggaagggcg gggccacgac ggtctacgcg ggctggtccg agctcgactg cgacgacgtc
    13621 gtgccgttca tcgacctcga gggcaccggc tggttcgtgg gtctggtgca ctcccacacg
    13681 ctggtggagg atgaggaccg gttgatttcg ctttcgggtg gcatcgtcta ccgctacatc
    13741 gaggaccagt tctccgcgtt caacacctcg ctgcagcagc gcgacgtctc ggtgctgccg
    13801 ctgtcgctcg cgctcagtta ctccgcgcgg cgggccgatg cgttccgggg ccggaatttc
    13861 gccacgctcc agggcgtgta caatctcgcg gccggcggcg ggaacgagtt ggaggacatg
    13921 tgggtcgatg cggaagacga ctacctgatc ggccgcctgc aactggcccg cctgcagccg
    13981 cttttcggca cgctcgacaa catgcagcgc caggtccacc agtggatcct ctttttcaag
    14041 gccgagggcc aatacgcctc ctgcccgctg atccccgccg agaaactcgc cttgggcggg
    14101 ttcaacaccg tgcgcggata ccagaccaaa gggtacttgg gcgacaacgg cctctacggg
    14161 acggtcgaac tccgcacgcc gatcctgctg gatatggtct cccacaccct cggccgcaag
    14221 ccgggctcca ccccgctcga ccggttgcag ttcgtggcct tcaccgattt cggcttcatc
    14281 gagttcatcg acccgctgcc cggcgtcgat gacaacgaaa ccctcctgag tgtgggcgcg
    14341 ggcgtgcgtc tcgccgtga
//
LOCUS       DAOSRB010000055        14144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4378_length_14144_cov_7.025836, whole genome shotgun sequence.
ACCESSION   DAOSRB010000055 DAOSRB010000000
VERSION     DAOSRB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14144
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4378_length_14144_cov_7.025836"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLZ74_04565"
     CDS             complement(<1..214)
                     /locus_tag="PLZ74_04565"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOR97628.1"
                     /translation="MNRIDQTFRTLSAARRKALVGYFTAGDPDFDTSLQILLAACAAG
                     VDVLEIGVPFSDPTCDGPTIQSASARA"
     gene            complement(242..1447)
                     /gene="trpB"
                     /locus_tag="PLZ74_04570"
     CDS             complement(242..1447)
                     /gene="trpB"
                     /locus_tag="PLZ74_04570"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOR97629.1"
                     /translation="MNFSTKPYPDATGHFGPYGGRYVGETLIAALTELESAWNDARND
                     PGFLDAFRAMLTHYAGRPTPLDFAGRLSDHIGGARIYLKREDCAHTGAHKINNVIGQG
                     LLARRMGKRRVIAETGAGQHGVATATIAARFGMGCTVFMGAEDVRRQAPNVLRMRLLG
                     AEVVPITSGTSTLKDAMNEALRYWVAEVRDTFYIIGTVSGPHPYPVMVRDLQRVIGDE
                     ARRQMLEQVGRLPDLLVACVGGGSNAMGLFYPFLDDPCEMLGAEAAGEGLDTPHHAAS
                     ISGGSPGALHGAMTYLLQNDEGQIQEAWSISAGLDYPGVGPEHALLHDLGRVRYEGVT
                     DREAVEAFKLLCQFEGIIPALESSHALAAALREAAARPRDQAVLVCLSGRGDKDLDHV
                     TAYMNEHNL"
     gene            complement(1458..2618)
                     /locus_tag="PLZ74_04575"
     CDS             complement(1458..2618)
                     /locus_tag="PLZ74_04575"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOR97630.1"
                     /translation="MSHPKLENVNIESVVLLATPREMKRAVPQTSASIATVTRGRETI
                     ERVLEDEDHRLLAIVGPCSIHHLESAETYARKLKQLSDEIADTICVVMRVYFEKPRSV
                     LGWKGLINDPYMNDTFHIEEGIRMARHFLMRVAEIGLPAATEVLDTLMPQYIGDLISY
                     SVIGARTAEAQTHREIASGMSTPVGFKNSTDGSLVGAINGIHAAMGYHHFLGVTEDGL
                     PAVFSTTGNPYPHIVLRGGKRPNYDAASIAFCEAALRTARLPVQIIVDCSHGNSEKQP
                     DRQGMVLRDLLTQIEQGNRSLRGFMLESQLEWGSQPMPKDPGQLNPGVSVTDACIDWE
                     TTEVLLREARDRYKVVIEERYTSYAGHARTPPSFPVRRALRRFRLTNPPLFL"
     gene            complement(2646..4121)
                     /gene="trpC"
                     /locus_tag="PLZ74_04580"
     CDS             complement(2646..4121)
                     /gene="trpC"
                     /locus_tag="PLZ74_04580"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOR97631.1"
                     /translation="MTYLEKILASKRDEIARAKAHTTRAQVERLAAARRDTPRGFAAA
                     LNASGVRVIAELKRASPSAGDIRPDLDPADLARRYAEGGAAALSVLTEPAFFKSSADD
                     LRQAREAVALPVLRKDFILDPYQVYESVALNADAILLIVRIVDDDTLHALYTLACSLG
                     LDVLTEVFDEEDAARARALGATLVGINNRDLARFSTDTTRTARLAALFPPDVSIVALS
                     GIRTSDDIRQTLACGIRRFLIGESLVRQPDPVATLRAWTTLHPSFPLSPMLPNLKICG
                     ITEHATARFCAEMGVGALGAVFYAKSPRYVTPSQARALFEGLPGHVARVGVFANCLPD
                     AMLAAAREARLTTLQLHGQESLADIRSLLRSGYRVIRTLSATGDSLLKIAQEIPTEAG
                     ILVECGQGPLPGGNAAVWNWAEAAPLAAVRPFALAGGLTPDNITDALRGSRAAACDVS
                     SGVETRPGVKNHDAIQKLVNALRKPAVTSGIQPFWLPSPNS"
     assembly_gap    4316..4415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4510..4953)
                     /locus_tag="PLZ74_04585"
     CDS             complement(4510..4953)
                     /locus_tag="PLZ74_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015127340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOR97632.1"
                     /translation="MKSEIDTLTGLIIGCAMEVHKTLGPGFLESVYEKALAIELGLQG
                     LAYERQLPIHAQYKGHSVGDFVADLLVEKKVLVELKASQALHDRHGIQLVNYLTATGI
                     DDGLLLNFGADSLQFKRKRRTYTPPHSPSPQSRQSCQSCQSCNPG"
     gene            complement(5396..6418)
                     /gene="trpD"
                     /locus_tag="PLZ74_04590"
     CDS             complement(5396..6418)
                     /gene="trpD"
                     /locus_tag="PLZ74_04590"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOR97633.1"
                     /translation="MNYSTLLKKLIARRDLTENEAFEAVTAILAGEMTEGQIGAFLAA
                     LAAKGETVDEVVAGARAMRTAAIRVQSLNPNTLDTVGTGGDGGMTFNISTTVAFVAAG
                     AGATVAKHGNRASSGKSGSADCLEQLGLNLDADPEIMEQALNEIGITFLFAPSFHKAM
                     RHAGPVRKQLGVRTLFNLLGPLTNPAGAPCQLIGVFAPDLTEIFAQALQKLGSRRVLV
                     VHGHDGMDEITLTAPTRCSELRDGRIKTYDIDPLALFDRFCTPSDLEGGTPADNAAIT
                     RALLSGGLHGPKRDIVLINAAASLLAGNRATDLADGIRKSAESIDSGAALDKLEQLIA
                     FSRQAM"
     gene            complement(6441..7028)
                     /locus_tag="PLZ74_04595"
     CDS             complement(6441..7028)
                     /locus_tag="PLZ74_04595"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOR97634.1"
                     /translation="MILMIDNYDSFTYNLVQYLRELAADVTVYRNDALTVQDITTLAP
                     DAIVISPGPGRPEDAGVSCDVVRAFAGQIPILGVCLGHQTIGLVFGAAITYAKQVMHG
                     KTSEITCDGQGLFKGLGDRPFKAMRYHSLVIDPDTLPACLQITARSEDGEIMGIRHTT
                     HLLEGIQFHPESIMTPVGKRILRNFLKLATAPCPS"
     gene            complement(7040..8560)
                     /locus_tag="PLZ74_04600"
     CDS             complement(7040..8560)
                     /locus_tag="PLZ74_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOR97635.1"
                     /translation="MRLDMFPAREAFTETLQTATVIPVGMKILADAETPVSVLSRFAN
                     ENANVFLFESAEGGERWGRYSFIGISARATIRIYREEVVCRQGITETRIPHRGDPLAV
                     LRDRMRPYTLAPMPGLPRFCGGLVGYFTYEMIHFFEPRVPNTLPQERPMAELFIPDTL
                     LIFDNAEHTLTVVALAFREDDADPDALYTAAVRRVHEVLDVLALPADVTMRLHKTPIR
                     MPQPAHPPERFQEQVEIVKRHIREGDIIQCVISQRFVGPAPDDVVSLYRAQRYVNPSP
                     YLFFLKSAECTLIGSSPETMIRLDNRTACLRPIAGTRPRGATEQEDRKYADALLQDEK
                     ERAEHLMLVDLGRHELGRVARTGTVQVTDLMVIERYSHVMHLVSNITADLEPACDAFD
                     LLRSSFPAGTLCGAPKIRAMEIITDLEEEPRGPYGGAVGYISFDGNMDFAICIRTAVV
                     EHGRLTVRAGAGIVADSDPETEHRETVNKAGAMARAIELLKRVQENELSPARPVKP"
     gene            complement(8648..9232)
                     /locus_tag="PLZ74_04605"
     CDS             complement(8648..9232)
                     /locus_tag="PLZ74_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97636.1"
                     /translation="MNKLHQLRTIIASLDETLVNAFCGRSRFRMNLDLYNEPKRTLSI
                     SETATLFGATATIAGRVRILRPLYVNTLLPHLCAPGTDEDRRKGIAADANCMNALVQR
                     LNLSVHVAALKREEIPEPLRAPLMMRDPVALEEAITNHAVEAEVIARILDMSRGQHAC
                     APDVPERIAHLYAGWIIPLSRKIQVHDLLASHHR"
     gene            complement(9240..10091)
                     /locus_tag="PLZ74_04610"
     CDS             complement(9240..10091)
                     /locus_tag="PLZ74_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOR97637.1"
                     /translation="MTPHPLLYLGPEGTHSHEAALRYADAGARPTACLSHFDLFDRLC
                     RSRARVRPVAVVPVENSSEGPVTQTLDQLASHPGVTIAASFSLAVRHHLLTGPDTASL
                     KAVQRVYSHPQALGQCHAALRRLVPHAEPMPESSTAAAARRAASEPGSAALASADAAR
                     IYGLRVLRRNLQDSRTNTTRFFAVTAHRRTRPAARPAGEVRSLIHLVIGNRPGALLHA
                     LSPFDAAGVNLTFIQSRPLPNRPWEYGFFIEAVTDWRSPAAEAAWRLILALSESGRII
                     GTYPILP"
     gene            complement(10099..11139)
                     /gene="aroF"
                     /locus_tag="PLZ74_04615"
     CDS             complement(10099..11139)
                     /gene="aroF"
                     /locus_tag="PLZ74_04615"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOR97638.1"
                     /translation="MIIVLKRHATETDIASVSDLVCSLRYQPRVIRGVEQTVIACIGD
                     ELSNRSLEVLNNLPAVESVFPVQKKFKLVSREYHPADSIVNVRNNLIGGGNIQVIAGP
                     CAIESFDQFRTAVADLSRCGIKIIRAMPFKPRTSPYDFQGLKLDGVKIMRDIKAEFDA
                     ALVSEVPGPAQIEILRDVADMFQIGARNAQNYDLLEHLAHAGKPVLLKRGMASTIEEW
                     LTAAEYLVVNGCPQVVLCERGLRSFDKTTRNLLDIGAVAVARNLSHLPVLVDPSHAAG
                     TRSLVMPLSRAGLAAGADGLIVEVHPDPVNAFSDATQQLESATFDRFLAELEPWIALA
                     RAQRALPNHQPA"
     gene            complement(11451..12734)
                     /locus_tag="PLZ74_04620"
     CDS             complement(11451..12734)
                     /locus_tag="PLZ74_04620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR97639.1"
                     /translation="MRILFILPGSGGSFYCQNCLRDTVLAAALQRRGHPVTLMPLYLP
                     ATAAMPAPEEVPVFYGAVTLYLRHRFAGLRRLPRSWFKPLDRWPVLRLAARFAGSTSA
                     SGLEDLTLSMLRGPDGHQADELNRVAEWVAGLPASERPSVILLSNALLAGLARTLSEA
                     AGCPVACWLQDEHVWLDAMAPQMRAAALRILRDDARAIDRFLAVSSSYAERMTDLLAL
                     DPARVRVVIPGVDPEAYAPADTTRRPATLGFLSRLSAEEGFDRFVDAFLLLRCDPRFT
                     GTRLAATGGPSADKRFLTRQRRKLADAGLLTDTEISPGQFSTDRFGFLSRLTLLSVPG
                     GAVPEALGYYALEALAAGVPVVLPAQGAFPELVSRAGCGTLLPDTRPESLAQAWAGLL
                     SDPERLRRDSEQGRRAAASIFSHDAAALALEQALA"
     gene            12921..13463
                     /locus_tag="PLZ74_04625"
     CDS             12921..13463
                     /locus_tag="PLZ74_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97640.1"
                     /translation="MKVSKGMVGLLVLLGVGLVLPVRGEGQERAYTPFMFSLVTPLQV
                     PFRDFNVGGLRINAIYGECHDFDGLDISTVGRATGHGNGFQAALLANIVDGDGFGMQV
                     APVDYVTGEYVGLQIGVANFAEKAQALQIGFYNGANHIDGCQIGVINTTRTMMGFQLG
                     LINVIQDNDVPFMPILNCYF"
     gene            13499..>14144
                     /locus_tag="PLZ74_04630"
     CDS             13499..>14144
                     /locus_tag="PLZ74_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97641.1"
                     /translation="MKNRAWLTFACAAAMAACAVRAQVVTEAEKLDPNASAELQAFKA
                     RVKAANPGMEVVSVEADVVKRAVLAPQPQPQNPKIAIFVKNQTRVPGMDDAVDGVRDR
                     ISAELAGAGLIVLDKAEIADGFNRFKITTAEERAGLVNGIFTGGSTVRVAQMLGCEYV
                     MLVSIIGASHTARMAGDKAVTVYTLRMTTKVNEAANGSSVYGSNWTNKLPVPGQY"
BASE COUNT         2534 a         3993 c         4912 g         2605 t
ORIGIN      
        1 atgcgcgggc cgaggccgat tggatggtgg gcccgtcgca ggtcggatcg gagaacggta
       61 cgccgatctc cagcacgtcc acccctgcgg cgcaggccgc gagcagaatc tggaggctgg
      121 tgtcgaaatc cgggtcgcca gccgtgaaat agccgaccag cgctttgcgc cgtgcggcgc
      181 tgagggttcg gaaggtttga tcaatgcggt tcataatcaa ggaggagagg gaagtcagga
      241 attagaggtt gtgctcgttc atgtaggcgg tgacgtgatc gaggtctttg tcgccgcgtc
      301 ccgagaggca gaccaacacg gcttggtctc tcgggcgtgc cgcagcctcg cggagtgcgg
      361 cggccagtgc gtgcgaactt tcgagcgcag ggatgatgcc ctcgaattga cagagcagtt
      421 tgaaggcctc gaccgcctcg cggtcggtga cgccctcgta gcgcacgcgt ccaaggtcgt
      481 gcagcagggc gtgctccggg ccgacgcccg gatagtccag tccggcggag atcgaccagg
      541 cctcttggat ttggccttcg tcgttctgca gcagataggt catggcgccg tgcaacgcgc
      601 cgggcgagcc gccgctgatc gaggccgcgt ggtgcggcgt gtcgagccct tcgcccgcgg
      661 cttccgcgcc gagcatttcg cagggatcat cgaggaaggg gtagaaaagc cccatggcgt
      721 tactgccccc tcccacgcag gcgaccagca ggtcgggcag gcggccgacc tgttcgagca
      781 tctggcggcg ggcttcgtcg ccgatcacgc gctggaggtc gcggaccatc acggggtagg
      841 ggtgcggacc cgacacggtg ccgatgatgt agaaggtgtc gcgcacttcg gcgacccagt
      901 agcgcaatgc ctcgttcatg gcgtccttca gcgtactggt gccggacgtg atcgggacca
      961 cctcggcgcc gagcaaccgc atgcgcagca cgttgggcgc ctggcggcgg acatcctcgg
     1021 cccccatgaa cacggtgcag cccatgccga aacgtgccgc gatggtggca gtggccacgc
     1081 cgtgctggcc agcgccggtc tccgcgatca cgcggcgttt gcccatgcgg cgggcgagga
     1141 gaccctggcc gatcacgttg ttgatcttgt gcgcgccggt gtgggcgcag tcctcgcgtt
     1201 tgaggtaaat gcgcgcgcca ccgatgtggt ccgagagacg cccggcgaaa tcaagcggcg
     1261 tgggacgacc cgcgtagtgg gtcagcatgg cgcggaacgc gtccaggaaa ccggggtcgt
     1321 tccgggcgtc gttccaggcg ctttccaatt ccgtgagcgc ggctatcagc gtttcgccga
     1381 cataccgccc gccgtacggt ccgaaatggc ccgtggcgtc gggatagggt tttgtggaga
     1441 aattcatgcg tttcttttta tagaaacaac ggtgggtttg tcaagcggaa tcggcgcaat
     1501 gcccgcctca cggggaagga ggggggggtg cgggcgtggc cggcgtagga ggtgtaccgt
     1561 tcctcgatca cgactttgta gcggtcgcgg gcttcgcgca gcaggacttc cgtggtttcc
     1621 caatcgatac aggcatcggt gacggagacg cccgggttga gttgcccggg atccttgggc
     1681 atgggttggc tgccccactc cagctggctc tcgagcatga accctcggag ggagcggttg
     1741 ccctgttcga tctgcgtgag cagatcccgc agcaccatgc cttggcgatc gggttgcttt
     1801 tcggaattcc cgtggctgca atccacgatg atctgcacgg gcaggcgcgc cgtgcgcaag
     1861 gccgcttcgc aaaacgcgat actggcggca tcgtaattgg ggcgtttccc gccgcgcaac
     1921 acgatatgcg ggtaggggtt gccggtcgtg ctgaagaccg ctggcagccc gtcttcggtg
     1981 acgccgagga agtggtggta ccccatggcg gcatggatgc cgttgatggc gccgaccaga
     2041 gaaccgtcgg tcgagttctt gaagccgacc ggtgtggaca tgccgctggc gatttcgcga
     2101 tgggtctggg cttcggcggt ccgcgcgccg atcacggagt atgagatcag gtcgccgatg
     2161 tattgcggca tcagggtgtc gagcacctcg gtggccgcgg gcaggccgat ctcggcgacg
     2221 cgcatcagga aatggcgcgc catgcggatg ccctcctcga tatggaaagt gtcattcatg
     2281 taggggtcgt tgatgagccc cttccatccg agcaccgagc gcggtttttc gaagtagacg
     2341 cgcatcacca cacagatggt gtcggcgatc tcgtcgctca actgtttgag tttgcgcgcg
     2401 tacgtctcgg cgctttcgag atggtggatc gaacaggggc cgacaatcgc gagcaggcgg
     2461 tggtcctcgt cttccagaac ccgttcaatg gtttcgcggc cgcgggtcac tgtggcgatc
     2521 gacgccgagg tctggggtac cgcgcgtttc atttcgcgcg gcgtggccag cagcacgacc
     2581 gattcgatat tgacgttctc gagtttgggg tggctcatgg gaaagaagga aggatgaagg
     2641 aggagttagg agtttgggga gggtagccag aaaggttgga tgccgctcgt aacggcgggt
     2701 tttcgtaatg cgttgacaag tttctgaatg gcgtcatggt tcttgactcc ggggcgggtc
     2761 tcgactccgg aggagacatc acatgcggct gcgcgcgaac cgcgcagcgc gtcggtgatg
     2821 ttgtccggcg tcaaaccgcc cgccagcgcg aaaggcctga cggccgccag cggcgcggct
     2881 tccgcccagt tccagacggc agcgttgccg ccgggaaggg ggccttggcc gcattctacc
     2941 aaaattccgg cttctgtggg gatctcctgc gcgattttca gcaaagaatc gccggtggcg
     3001 gaaagcgtcc ggattacacg gtagccggat cgcagcaggg aacggatgtc ggcaagagac
     3061 tcttggccgt gcagttggag cgtggtgagc cgcgcttcac gcgcggcagc gagcatcgcg
     3121 tccggcaggc aattggcgaa cacgcctacg cgcgcaacgt gcccgggcag tccctcgaaa
     3181 agcgcccggg cctgggaggg ggtcacgtag cgcggacttt tggcgtaaaa aaccgcaccc
     3241 agcgcaccca cgcccatctc ggcgcagaat cgggctgtcg catgttcggt aatgccgcag
     3301 attttgagat tggggagcat gggggaaagg gggaaggagg ggtggagggt ggtccaggca
     3361 cggagcgtgg caacgggatc aggttggcgg acgagactct cgccgatcag gaagcggcga
     3421 atgccgcagg cgagggtctg ccggatgtca tcggatgtgc ggattccgct cagggccacg
     3481 atggagacat cgggcgggaa cagcgcggca agacgcgcgg tgcgggtggt gtcggtgctg
     3541 aagcgcgcga ggtcgcggtt gttgatccca accagcgtcg cgcccaaggc gcgggcgcgg
     3601 gcagcgtcct cttcgtcaaa cacctcggtc aagacatcca agccgagtga gcaggccaac
     3661 gtataaagag catggagggt gtcgtcatcc acgatccgaa cgatcaggag gatggcgtcc
     3721 gcgttcaggg cgacgctctc gtacacttga taggggtcga ggatgaaatc cttgcgcagc
     3781 accgggagcg cgacggcttc acgggcctgc cgaaggtcat cggccgaact tttaaaaaac
     3841 gcgggttcgg tcagaaccga gagcgcggcc gcgccgcctt cggcataacg ccgcgccagg
     3901 tcagcggggt cgagatcggg acggatgtcg ccggccgagg gcgaggcgcg tttcagttcc
     3961 gcgatcacgc gcacgccgga cgcattgagc gcggcggcaa agccgcgcgg ggtatcacgg
     4021 cgcgcggcgg cgagccgttc gacctgagcg cgcgtggtgt gcgctttggc gcgggcgatt
     4081 tcgtcacgtt ttgaggccag gattttttcg aggtaggtca ttgtttagcc aggatttccg
     4141 acagcttcac ttcggcgggg agaatttcag gattgacagg attgacggga ttgtgtgggg
     4201 aggggtttag ccaggattac aggattgaca ggattgacgg gattgtgtgg ggtggggttt
     4261 agccaggatt acaggattga cgggattgtg tggggtgggg tttagccagg attacnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggggt ggggtttagc caggattacg
     4441 ggattgacgg gattgagggg gtggggttta gccaggatta cgggattgac gggattgagg
     4501 gggtggggtt tagccaggat tacaggattg acaggattga caggattgac gggattgagg
     4561 ggacggggag tgcggaggtg tgtaggttct gcgcttacgt ttgaactgca ggctgtcggc
     4621 accgaaattt aacagcaatc catcgtcaat gcccgtggcg gttaagtaat taacaagttg
     4681 gatgccatgc cgatcatgta gcgcttgact ggctttcaat tcaacaagga ctttcttttc
     4741 gacaaggagg tccgcaacga aatcacccac agaatggcct ttatactgcg cgtgaatggg
     4801 taattggcgc tcatatgcaa gcccctgtaa ccccagttca atcgcgagtg ccttttcgta
     4861 aactgactca agaaagcctg ggcctagagt cttgtggact tccattgcac atccgataat
     4921 taaaccagtt agagtatcaa tttcgctttt catgccttct ccttgtgatg cgtgatgcac
     4981 agttttatcc gattcttgtg ccatcccgtc aatcctgaaa tcccggctaa gtcccgtcct
     5041 gtcaatcctg taatcctggc taaaccccgt cctgtcaatc ctgtaatcct ggctaaaccc
     5101 cgtcctgtca atcctgtcaa tcctgaaatc ccggctaagt cctgtcccgt caatcccgtc
     5161 aatcctgtaa tcctggctaa accccgtccc gtcaatcccg tcaatcctgt aatcccggct
     5221 aagtcctgtc ccgtcaatcc cgtcaatcct gtaatcctgg ctaaaccccg tcctgccaat
     5281 cctgtcaatc ctgtaatcct ggctaaaccc catcctgcca atcccgtaat cctttccgtt
     5341 cccgccgaag tgaagctgcc ggaaatcctg acaaaagcca tctcatgtat ccattttaca
     5401 tcgcctgccg cgagaacgcg atcagctgtt cgagtttgtc gagtgccgcg ccggaatcga
     5461 tggactccgc agacttcctg atcccgtcgg cgaggtcggt ggcacggtta ccggcgagca
     5521 gcgaggcggc ggcattgatc agcacgatgt cgcgcttggg tccgtgcaac ccgccgctga
     5581 gaagggcgcg ggtgatggcg gcattgtcgg cgggggttcc gccttcaagg tcggaagggg
     5641 tacagaaccg gtcgaagagg gcgagcggat cgatgtcgta cgttttgatg cggccgtccc
     5701 ggagttccga gcagcgggtt ggcgcggtga gcgtgatttc gtccatgccg tcatgcccgt
     5761 ggaccaccag tacgcggcgc gagccgagtt tctgcagcgc ctgcgcgaaa atctcggtaa
     5821 gatccggcgc gaacacgccg atgagttggc agggtgcgcc cgccgggttg gtcaacggtc
     5881 cgagcaggtt gaaaagggta cggacgccga gctgtttgcg gaccggtccg gcgtggcgca
     5941 tcgccttgtg aaacgaagga gcgaagagaa aggtgattcc gatttcattc agggcctgct
     6001 ccatgatttc cgggtcggcg tcgagattca agccgagctg ttcgaggcag tcggcgctgc
     6061 cgctcttgcc ggaactggcc cggttgccgt gtttggccac ggtcgcgccc gcgccggcgg
     6121 ccacaaaggc gaccgtggtg gagatgttga aagtcatgcc gccgtcgccg ccggtcccga
     6181 ccgtgtcgag cgtgttcggg ttgagggatt gcacacggat cgcggcggtg cgcattgcgc
     6241 gggcgccggc aacaacttca tcgacggtct cgcctttggc ggcaagggcg gcgaggaaag
     6301 cgccgatctg gccctccgtc atttcgccag ccagaatggc ggtgacggcc tcgaacgctt
     6361 cgttttcggt caggtcgcgg cgcgcgatca gctttttgag cagggttgag tagttcatga
     6421 tggaaaggat gagggaaacg tcaggaagga cagggggcgg tggcgagttt gaggaagtta
     6481 cgcaggatac gcttgccgac cggcgtcatg atggattcgg ggtggaactg gatcccctcc
     6541 agcaagtggg tggtgtgacg gatccccatg atctcgccgt cctctgaacg cgcggtgatt
     6601 tggagacagg cgggcagggt gtcggggtcg atcaccagcg agtggtagcg catggctttg
     6661 aagggacggt cgcccagtcc tttgaacagc ccttggccgt cgcaggtgat ttctgaggtt
     6721 ttgccgtgca tcacctgttt cgcgtaggtg atcgcggcac cgaagaccag tccgatcgtc
     6781 tgatgtccga ggcagacccc cagaatcggg atctggcccg cgaaggcgcg gaccacgtca
     6841 caggagacgc ccgcatcctc ggggcggccc ggtccgggcg agatcacgat ggcgtcgggc
     6901 gcgagcgttg tgatgtcctg gacggtcagg gcgtcgttgc ggtacacggt cacgtcggcg
     6961 gcgagttcgc gcagatattg gaccagattg taggtgaacg agtcgtaatt atcgatcatc
     7021 agaatcatgg cggggttcct cagggtttga cggggcgggc cggggagagt tcattctcct
     7081 gaacgcgttt tagcaattcg atcgcgcggg ccatcgcgcc cgctttgttc acggtctcgc
     7141 ggtgttcggt ctcgggatcg gagtcggcca cgattccggc gcccgcgcgg accgtgagcc
     7201 gtccgtgctc caccacggcg gtccggatgc agatcgcgaa gtccatgttg ccgtcgaacg
     7261 agatgtatcc cacggcgccc ccatagggtc cgcgcggctc ctcctcgaga tccgtgatga
     7321 tctccatggc gcggattttg ggtgcgccgc agagtgtgcc cgcagggaac gaggagcgca
     7381 gcagatcaaa cgcatcgcag gccggctcga ggtcggccgt gatgttggag accaggtgca
     7441 tgacgtgcga gtagcgctcg atgaccatga ggtccgtgac ctgcaccgtg ccggtccgcg
     7501 ccacgcggcc gagttcatgg cgaccgagat cgaccagcat caggtgttcg gcacgctcct
     7561 tctcgtcctg caggagagcg tcggcatatt tacggtcctc ctgttcggtt gcgccgcgcg
     7621 gccgtgtgcc ggcgatcggc cgcaggcagg cggtgcggtt gtcgagccgg atcatggttt
     7681 cgggcgagga gccgatcagc gtacactccg ccgacttcag gaaaaagagg taaggcgagg
     7741 ggttgacata ccgttgggcg cggtagaggc tgaccacatc atccggggcc ggtcccacga
     7801 aacgctggga gatgacgcac tggatgatgt cgccctcgcg gatgtggcgc ttcacgatct
     7861 ccacctgttc ctgaaaccgt tctggcggat gtgccggttg cggcatgcgg atcggggttt
     7921 tatgcaggcg catcgtgaca tcagcgggaa gcgccaacac atcgagcacc tcgtggacgc
     7981 gccggacagc ggccgtgtaa agggcgtccg gatccgcatc atcttcccgg aaagcgagcg
     8041 ccaccactgt cagcgtgtgc tcggcgttgt cgaagatgag cagggtgtcg gggatgaaca
     8101 gttcggccat cggacgttcc tgcggcaggg tgttggggac gcgcggctcg aagaaatgaa
     8161 tcatctcata cgtgaagtat ccgaccagtc cgccgcagaa ccgcggcagt cccggcatcg
     8221 gggcgagcgt gtagggccgc atacggtcac gcagcacggc gagcgggtca cccctgtggg
     8281 ggatgcgcgt ttcggtgata ccctggcggc agaccacctc ctcccggtag atgcggatgg
     8341 tggcgcgggc ggagatgccg atgaaactgt agcggcccca ccgttcgccg ccctccgcgc
     8401 tttcaaacaa aaaaacattc gcgttctcgt tcgcgaaacg agagagcacc gaaaccggcg
     8461 tttcggcatc tgccaaaatc ttcatgccca ccggaatcac cgtggcggtt tggagcgttt
     8521 ccgtaaacgc ctcacgtgcg ggaaacatgt cgagtctcat catccactcc tgcctgattt
     8581 gttcaggtgt tactttatat agaaacgccg ctccggcgtc aacgggaaaa aatccgcgat
     8641 ctccgggtca ccggtggtgt gaggcgagca ggtcatgaac ctggatttta cgcgacaacg
     8701 ggatgatcca tccggcatag aggtgggcga tccgctccgg cacgtcgggt gcgcaggcgt
     8761 gctggccgcg gctcatgtcg aggatccgcg cgatgacctc ggcctcgacc gcgtggttgg
     8821 tgatcgcctc ctccagcgcg acggggtcgc gcatcatcag cggtgcgcga agcggttcgg
     8881 ggatttcctc gcgtttcagc gcggcgacgt gcacggagag attgagccgc tgcaccagag
     8941 cgttcatgca gttggcgtcc gccgcgatcc ccttgcgccg gtcctcgtcc gtacccggtg
     9001 cgcacaggtg cggcaggagg gtgttgacat agagcgggcg caggatgcgc acacggccgg
     9061 cgatggtggc ggtggcgccg aagagcgtgg cggtctcgct gatgctgagc gtccgtttcg
     9121 gctcgttgta caggtcaagg ttcatgcgga agcgcgagcg tccgcaaaag gcgttgacca
     9181 gagtttcgtc gagggaggcg atgatggtgc ggagttggtg cagtttattc atggtgtcct
     9241 caaggcagga tcggataggt gccgatgatc cggcccgatt cggagagggc caggatcagg
     9301 cgccaggcgg cctccgcagc gggagagcgc cagtccgtga cggcttcaat aaaaaatccg
     9361 tattcccagg ggcggttcgg cagtgggcgg ctctggatga aggtcaggtt caccccggcg
     9421 gcgtcgaacg gcgagagggc gtgcaggagc gcgcccggcc ggttgccgat caccagatgg
     9481 atcagggacc ggacctcgcc tgcgggacgt gccgctggtc gcgtccgcct gtgcgcggtg
     9541 accgcgaaaa agcgtgtggt gttcgtgcgg ctgtcttgca ggttgcggcg cagcacccgc
     9601 aggccgtaga tccgcgcggc gtccgccgag gccagcgcgg cgctgcccgg ttcgctggcc
     9661 gcacggcgcg cggccgcggc ggtgctcgac tcgggcatcg gttccgcgtg cggcacaagt
     9721 cggcggagcg cagcgtggca ctgccccaaa gcctgcggat ggctgtacac gcgttgaacc
     9781 gctttcagcg aggccgtgtc ggggccggtc agcaggtggt gccggaccgc gagggagaag
     9841 cttgccgcga tggtcacccc gggatgcgac gccagttggt cgagcgtctg ggtgaccgga
     9901 ccttcgctgg agttttccac tggcacgacg gccacgggac ggacgcgcgc gcgcgaacgg
     9961 cacaaccggt cgaacaggtc gaagtgcgac aggcaggcag tggggcgggc tcccgcgtcc
    10021 gcgtaccgca gggcggcttc gtgggagtgg gtcccttcgg gaccgagata aaggaggggg
    10081 tgtggagtca tcggaccctc aggcgggttg gtggttgggg agggcacgct gcgcacgggc
    10141 caacgcgatc cacggttcga gttcggcgag gaagcggtcg aacgtggcgg attcgagttg
    10201 ctgtgtcgca tcgctgaatg cgttgaccgg gtcggggtgg acctcgacga tcaggccgtc
    10261 cgcgcccgcc gccagaccgg cacgggacag gggcatcacc agactgcggg tgcctgcggc
    10321 atggctgggg tcgaccagca cggggaggtg cgagaggttg cgggccacgg ccacggctcc
    10381 gatgtcgagc aggttgcgcg tggtcttgtc gaatgagcgc aacccgcgtt cgcagagcac
    10441 gacctgcggg cagccgttga ccaccagata ctcggcggcg gtgagccact cctcgatggt
    10501 gctcgccatg ccgcgcttaa gcagcaccgg tttgcccgcg tgggccagat gctccagcag
    10561 atcgtagttc tgggcgttac gcgcgccgat ctggaacatg tcggcgacat cgcgcagaat
    10621 ctcgatctgc gccggccccg ggacttcgga aaccagggct gcgtcgaatt cggccttgat
    10681 gtcgcgcatg atcttcacgc cgtcgagctt gagcccctga aaatcatagg gcgaagtgcg
    10741 cggtttgaac ggcatggcgc ggatgatctt gatcccgcag cggctaaggt ctgccacggc
    10801 ggtgcggaac tggtcgaagc tctcgatggc gcaggggcct gcaatcactt gaatgtttcc
    10861 gccgccgatg aggttgttcc ggacgttcac aatcgagtcg gcgggatggt attcgcgcga
    10921 cacgagtttg aattttttct gcaccgggaa aaccgattcc acggccggca ggttgttcag
    10981 gacctccagc gagcggttgc tcagttcatc cccgatacag gcgatgaccg tttgttcaac
    11041 gccgcggatg acgcgcggct ggtagcggag ggaacagacc agatcgctga ctgaggcgat
    11101 gtcggtttcc gtcgcgtgac gtttgagtac gatgatcatg agcgtttccg ttttccgtga
    11161 ggggggtgaa aaaacaacaa ccgcgtgtcc gaagttgccg gacgcgcggt ttgtttcaag
    11221 tctgttcccg tattacccaa gcctaccggg tcgacccgaa ccgtgtgtcc ggcaacctaa
    11281 agtagctaaa gtaaaaatag gaacgtgtgc tgttcatgag ttgtgcgcgt atttaaccgc
    11341 ttcccccggg atgtgtcaag gggctgctat gcgaaaaaca aaatccttaa tcgacaggcg
    11401 cgaccggaga cgacgggtga acacctgtta ccggccactt gccgcgcggt tcaggcgagg
    11461 gcttgttcga gcgcaagggc tgcggcgtcg tgcgagaaga tggaagcggc cgcgcgcctg
    11521 ccttgttcgg agtcgcggcg cagccgttcc gggtcggaca gcaacccggc ccaggcttgc
    11581 gcgagcgatt cggggcgcgt gtcggggagc agtgtgccgc atccggcgcg ggagaccagt
    11641 tccggaaagg cgccctgcgc gggcagcacc accggcacac cggcggctag ggcctccagc
    11701 gcgtaatatc ccagtgcttc ggggacagcg ccgcccggca cggagaggag ggtcaaccgt
    11761 gacaggaatc cgaaccggtc ggtggaaaac tggccgggcg agatctcggt gtccgtcagc
    11821 aggccagcgt ccgcgagctt ccgccgctgg cgggtgagga accgtttgtc ggccgagggt
    11881 ccgcccgttg cggcgagacg cgttccggta aaacgcgggt cgcaccgcag cagcaggaag
    11941 gcatcgacga accgatcgaa gccctcttcg gccgagaggc gcgagaggaa gccgagggtg
    12001 gccgggcggc gggtggtgtc ggctggcgcg taagcctcgg gatcgacgcc cggtatgacc
    12061 acgcgcaccc gcgcgggatc cagggcgagc aggtcggtca tgcgctccgc gtaggacgag
    12121 ctgaccgcga ggaaacggtc gatcgcgcgc gcgtcgtcgc gcaggatccg taatgctgcg
    12181 gcacgcatct gcggcgccat ggcgtcgagc cacacgtgct catcttgcag ccagcaggcc
    12241 accggacagc ccgccgcctc actcaaggtc cgggccagcc cggcgagcaa ggcgttggag
    12301 agcaggatca cggagggccg ttccgacgcg ggtagtcccg cgacccactc cgccacccgg
    12361 ttcagctcgt ccgcctggtg cccgtccggt ccacggagca tcgagagggt gagatcctcc
    12421 agtccggagg cggaggtcga acccgcgaag cgggcggcga ggcgcaggac cggccaacga
    12481 tccaacggtt tgaaccagga gcgcggcaga cggcgcagac cggcgaagcg gtggcgcagg
    12541 tagagcgtca cggcaccgta gaagacgggc acctcctccg gcgcgggcat cgccgcggtg
    12601 gcgggcaggt agaggggcat aagggtcaca ggatggccgc ggcgttgcaa cgctgcggcc
    12661 aagaccgtgt cgcgtaggca gttctggcaa tagaacgaac cgcccgatcc cggaagaatg
    12721 aaaaggatgc gcatccggag gccagcgtag caaaacgccc ggcggcgttc acgttttaaa
    12781 cgcccgcagg caagaatctg attgaacgga cgtttatttt ggggcatact ctaaaccatc
    12841 tgatacctct ttgaccgcgc acgggacgca aaggcggagg cgggtgcggc ggggggtgtc
    12901 ttcaagccaa ggaggccgag atgaaggtat cgaaagggat ggttggttta ctggtgctgc
    12961 tgggcgtggg tctggtgttg ccggtaaggg gcgaggggca ggagcgcgcg tacacgccgt
    13021 tcatgttcag tctggtcacg ccgttgcagg tgccgttccg ggatttcaat gtgggtggcc
    13081 tccggatcaa cgccatttac ggcgagtgcc atgacttcga cggtctggac atcagcacgg
    13141 tcggccgcgc taccgggcac ggcaacgggt ttcaggccgc gctgctggcc aacatcgtgg
    13201 acggcgacgg gttcgggatg caggtcgcgc cggttgatta cgtgaccggc gagtacgtcg
    13261 ggctgcagat cggcgtggcc aacttcgccg agaaggctca ggcgctgcag atcggatttt
    13321 acaacggcgc gaatcacatc gacggatgtc agatcggcgt gatcaacacc acgcgcacga
    13381 tgatgggctt ccaactcggg ctcatcaacg tgatccagga caacgatgtg ccgttcatgc
    13441 ccatccttaa ctgctatttc tgaatccgaa ccccgtacgg cgggaagaga ggttgcatat
    13501 gaaaaacaga gcatggctga ctttcgcgtg tgccgcggca atggccgcgt gcgccgtccg
    13561 cgcgcaggtg gtgaccgagg cggagaaact ggaccccaac gcctcggctg aattgcaggc
    13621 cttcaaagca cgggtcaagg ccgccaaccc cggcatggag gtggtctcgg tcgaggcgga
    13681 tgtggtgaag cgcgcggtac tggcgccgca gccgcagccg cagaacccta agatcgcgat
    13741 ctttgtgaag aatcaaacgc gtgtgcccgg tatggatgac gcggtcgacg gggtgcggga
    13801 ccgtatctcg gccgagctgg ccggcgcggg cttgatcgtc ttggacaagg ccgagatcgc
    13861 ggacggcttc aaccgtttca agatcacgac tgccgaggag cgcgcgggat tggtcaacgg
    13921 cattttcacc ggcggctcca ctgtgcgcgt ggcccagatg ctgggatgcg agtacgtcat
    13981 gctggtgtcg atcatcgggg ccagccacac tgcccgcatg gcgggcgaca aagcggtcac
    14041 ggtctacacg ctgcgcatga ccaccaaggt gaacgaggct gccaacgggt catccgttta
    14101 cggttccaac tggaccaata agttgccggt gcccggacag taca
//
LOCUS       DAOSRB010000056        14134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4379_length_14134_cov_6.848995, whole genome shotgun sequence.
ACCESSION   DAOSRB010000056 DAOSRB010000000
VERSION     DAOSRB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14134
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4379_length_14134_cov_6.848995"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLZ74_04635"
     CDS             <1..428
                     /locus_tag="PLZ74_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR97642.1"
                     /translation="EEVAHAREEGVVFHMLQNAVRILGDVQDRVTGMECLRYELGEPD
                     ASGRRRPVPISGSEFQMEVDTVIVAIGNDSNPLISQTTEGLAVNKWGNIIVDENGKSS
                     IDGIYAGGDIVLGAATVILAMGEGRRAAAAINAFLADKT"
     gene            465..1127
                     /locus_tag="PLZ74_04640"
     CDS             465..1127
                     /locus_tag="PLZ74_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010283908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOR97643.1"
                     /translation="MTHSTPRRYTISEEVAHSAIHALGSHLGAAMLALMVVFGVQSGE
                     QVAWKVVSAALFGFSIILLYTVSSAYHAITHPRTKQVLKVCDHMAIYILIAGSYTPFT
                     LVTLRPEYPVIAWTIFGIVWGLTIMGIVFKVFFTGRLRLVSTLAYVGMGWIIVFAIKP
                     LMATLPTAGLLWLLIGGLCYTLGVIFYLWRLMPFHHAVWHLCVLAGTLCHFFAVLFCV
                     MM"
     gene            complement(1153..4062)
                     /locus_tag="PLZ74_04645"
     CDS             complement(1153..4062)
                     /locus_tag="PLZ74_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOR97644.1"
                     /translation="MKPRIRLSLLLLSAIIVATASAVNSDGVLGRKSTWCPDTVADLP
                     SKIICEGRYKWHLQGVDSDGQFLYWSFSDRVVKSDKLGRRVAVSAIDKLHYGDLCVVD
                     GVVYVAFNGGEFNKETGALNRVLAFKASDLSRCGEWDIPEVVHGCGGITFADGCFYVV
                     GGLPPNHVKNYVYRYTKDFKFDRRIDLDTGYTMLGIQTINFMHDEFLVGIYPDRHDPE
                     IGLLRFSKDFSDFERLSIGAWVGAVEIDGKFYQARDEANGPEGTNRETGVLYLASFPK
                     KPSEPFTGGLWKTFTYPEPDTKPILFSGWSRSENAKAADYGIFLDVGFADGTWYWDAA
                     ADFPGGTHTWRRAAGVFVPEKPVKEVKFYALLRHGVGKAEFRNLKLERRESAPGGEII
                     VENVRSDFPYSDGEAVHEIRLDGRKVTERDYVRPASIGAPRVARDFPAGKAVVWTASS
                     MRKVTPLTFPSTAEMTEKNRRIDLELAGNERESAQILVSAGKGGAWKSGTLKIGTLTD
                     KSGREFRGEIKWERVGYVTRGHEGLIHPGSPDPAERWIPDPLLPAAPFKVRENATQGL
                     WLTVYAAPDATPGLYCGIVEVRENGDTMASIPLSIKVCPFALPRTFGTLNSFTVMDSF
                     TRAQYPDCFEKMKRQSWDVMLDHRLNPDNISRTTPPDVEHLKYAVKRGLNLFNILNVV
                     SEPKDPNVKIVYEETAETYADPKFYEAFAQRLKPYVEEYRKAGLLKYAYVYGFDERES
                     DCFPWIADIYRKYKRDFPDVPLLSVARAYEFAVNGKADEKYAYDADWCCPNIRSYQPE
                     FSARMREKGRKVWWYSTVAPFYPGPNFDSLESVPLEGRMLVGFMTDWRGADGFLYWHV
                     NYWPNNAKCLDESDTFFPDWPTYTPRSRYSSGGGVLLYPGAEHVLPSIRFAMVRDGVE
                     DREWTKLAMARSGEDRTRAVLTEAVRSVEDYERDPEKLLKIRSKIGALISEGKPK"
     gene            complement(4121..6565)
                     /locus_tag="PLZ74_04650"
     CDS             complement(4121..6565)
                     /locus_tag="PLZ74_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="botulinum neurotoxin N-terminal receptor binding
                     domain-containing protein"
                     /protein_id="HOR97645.1"
                     /translation="MAKKNVMVTLMLSLTMVVGLQATAKTVAWYRFEEAPSGTRTTAD
                     SVIVNSVDPTKLPGHAKVQTPILWSGAQQLTTPESLMPLYTGTFNSDLKVVDPVSGEI
                     YENNGALFFASADTSNHESAGSTVLIDNDAALHLKSMTIEFFVKIPAGTHSVDRYLLA
                     QRSSNNSVFTWNVMYKNELVYAEIDANGTRYTVYDAYSHVNDGKWHHIAITIDDDSKT
                     FSLYVDYQIQTWCMQKTYTGDICYDNAQPLALGPHIQCYYGIIEHTLDELRISDRVLG
                     VGEFLRARPIAAPSAASTPETLFLLDFDGGTTYTVYTDFAKEITNAPPAYRNKSWPSS
                     VWAEYFATTYTKSSVTSSVPVQLVGDVATALVRSSLAGTNFVTNAVSCLTSADATTND
                     WADSVVLPKAITRDMVFGDDVTIEVSFKLPSAGDIASSSGGPHLFCMLGGFQILIPGN
                     AATWEKGMLSAFVGSTQLTGNWVNHPQFIPRNVRDDQWHHLALSYDKTANRADLYLDG
                     VWRLGADNIVFPDVGSMWYLYRRELMLGGSYYDERYVLNMHYDNLRITRGVLKPYQFL
                     TSRAVECGAVLGWASYDDTFALTPYADFFATPAAAAFTTGGVAPVLDSDRPARTIFDG
                     KHGEPVRTANAGSIRFNGSMLTYPGHDLVADQADVTVEFFLKSAAPVAGAGIVRLNRA
                     SENEAGDISWALSFADASGNLAVAVDTDTQTGQSHTFGGAFGDGAWHHVAVTFAADGD
                     DTLVTVYRDYQSIGSYTFTGNMMCRFKEMNLMLGAGAVDVGFDGWIDELRITPGTLAV
                     EDFLWLKRKGFTLLFK"
     gene            complement(6621..7268)
                     /locus_tag="PLZ74_04655"
     CDS             complement(6621..7268)
                     /locus_tag="PLZ74_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97646.1"
                     /translation="MKTLVLIAVSVAAFGLFAAEDICFAAGKWDQKAWWNVANPRWNY
                     RHGFEQAEDCILNECPGTKIADWYSAMVMTNRFSEAVSISARMSFDDRFAPGILIAPE
                     LVDGPTGKELRDHFEIILFDGGINVWRYKWTETGFVITKPAFVVTPFAPRTPLTVTVT
                     IKERNVVGGGKTRQMTVTCGSCQFGFRSDDLPASFYAGINGNEGRCRFYSFGVGD"
     gene            complement(7273..11589)
                     /locus_tag="PLZ74_04660"
     CDS             complement(7273..11589)
                     /locus_tag="PLZ74_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97647.1"
                     /translation="MSKIIQGLVGVLTLGFAFAVQAGGTLADPAASADWNADSTWSGG
                     AKPVAGEAVMVLPTGDITLTMSADSTNDYASLYFRSSVAKTTAASLTFDGRGYKLLMP
                     YSANGYYVGAPFKILDADEKEAFKLDDSKLDAADKIKAAPILFDDARFKISQDTAGAV
                     TFRMDSGTLNACNPAGTAYALREILMSNGSQPFRFELVNGAKAVFPKLYAGYTKPGSM
                     LFDNAELTVHGALQFGRDNYYYSESALVLAATNTTFVCGGAVGFYHTATNVFKDCTID
                     IAGEMVNSWNSSVTFENCTLTATNTLIISAAGNNGEPAEMTFDGGTYSFGYPQIGFQS
                     NSDGKMTVKGGAVVDYVKSGSSQGQHIGWRGTGVIDVVSGTFRTPRSTYCQLYVGKYA
                     DTYGTGWGRLNVHDSGKVEIRMGTNSWAAGATDGLAVGGGGHGEVHVYGGEIIAPRIN
                     VGFETTALTTTSFIHQVGGRVSVNTLNDAKYSRVALMAYSGAAGGHWNDRDIMNRAAY
                     VLDGGVFEAARILGGKGSQSQGGSGHSRFSANGGTFRQNMNPSTYASMEGLDVAEFGS
                     RGLVFDSNGHDVKIAQNLINKPGESGLFVKTGRGTLTYAGECSVSNLVVSNGTWQLDA
                     GATVSSALRVVTGGTFSLVGDPTSATLASLAVTNATIALDPGDTITVQGNASFKGLRL
                     VFSSAPAMYTAQTVFVVQGTLDDTSVSELQRAFCDVALADGTHAQFQTATEGGVTSVT
                     VTIEDNAEPIGADATLTWTGSGLWANSGNWTPNGMPDADKKAAFTSTAAGKTVTVDSA
                     AVAGALSFGADGYSLTGSGSVEIAGAQGAAQIVGAAGVNTVSVPMTFNTLVEAPIAAG
                     AAVNLDGDISHGGILKTGDGMLALRGDNTFDLDVRLAAGTTLVGSDGALGPAESAWAA
                     RVQGGTLAFDADGNINLARPVTFDTSAMVVIDARTNATATLDVTAGGKLVKRGAGTLT
                     VPLTQNIVWDTSNLTGGQDYTEEVSFNADGTATVTKAHPIMIVEGGLVIRSADGTPKT
                     LTTRGNLIIGEKCTTATTSPELTLDGVQLITPLETTFGIGYCNGSEWNANTLYPKLTL
                     VNGASVKTHNFTLGNRCRSGAVPTLAMTNATITTTYALYLNGGANGSEKTVVRAKDST
                     LLISTSSGKLHYYGAADHDFDNCMIDTTPSGGFELVFSYGYNSIYPSGEVRFRNGSVF
                     RAKSVNMNGVYTTGDGLPFLRKPFTLVFDNATWDYGASDITITSTVGAETNLFIRTTG
                     GGIKLNTPSGTMFATDSKVIGDGGIAKTGAGTLKFGNGSYAFTGTCDIEEGTVNLSVA
                     GMVTNAVFGGSGTVSGGTLRDTTIIATIAENGTVAATPLFENCAFVGRTDVDLGRSEN
                     PLVRPYPQDVVVAHYTGTVPDVSRWRVTGTGVTNLRGTFTAEGGVIRMSLNHSGFTIT
                     IK"
     gene            complement(11626..13632)
                     /locus_tag="PLZ74_04665"
     CDS             complement(11626..13632)
                     /locus_tag="PLZ74_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97648.1"
                     /translation="MKRLVFGVTMWVVFGISAGGGEFEGYSKREFGAPTELRAFDNAE
                     FDDLTKFGRENANVEYGKFGFNGNGGLRIRPLANGRTHRFQQRVALAKGKRYIFSADV
                     HTHGKASAQIAVDTFWKDSRKYASGYGAWGASSKPLADGWKTFWVTFVAKEGSDKIDY
                     HFIAHCPGKNTYTEDDWIDIDNISLREDVPVWYFCNTWPVHNKIYCEEGRIRCHSWFF
                     GDFLEKDGKDVYELKLLRGDGASAAASPLARCVLAPDETGVMTAAFGKLDYRGPATLT
                     ATIYDTIHRQELGTKTLEIEICPAPDRSKHLFVQENGVVLRNGEPFMPFGFYTDLADP
                     KKYDAAKLEAELKKLHEAGFNAMIDYQSYSLRTGPRCETYYRLCEKYGIGVLTDAFGH
                     GSATTNAIDKSRALAAYLEKFPSLIGFYTMDEGAESVVPGLAATRRMLNEVCPGRMVN
                     ICNIMRAAPYLPAADIQGGDAYPIRLGGGGMMDTHNRVTRMQECGPAAIWWAPQCYNW
                     VNQNQEARTNAVLYRKLGREPTENELLAVALLNAADGVTGFFFYSLFDLYRGPIPEWV
                     DRRWTAMCNIAKVFKDLEPFILSGEKIIDVPHKDAKDPVRVAALSDGKGRWRVIVVGL
                     GKNHDTTFTLPASYGQLRGMCGNAVFANGIYTYKAEEYVCDLLR"
BASE COUNT         2875 a         4291 c         3998 g         2970 t
ORIGIN      
        1 tcgaggaggt cgcccacgcc cgcgaggagg gcgtggtctt ccacatgctg cagaacgccg
       61 tgcgcattct cggcgacgtg caggatcgcg tcaccggcat ggagtgcctg cgctacgagc
      121 tgggcgagcc cgacgcctcc ggccgccgtc gccccgtgcc gatctccggc agcgaattcc
      181 agatggaggt ggacactgtg atcgtggcta tcggcaacga ctccaacccg ctgatcagcc
      241 agaccaccga agggctggcc gtcaacaaat ggggcaacat catcgtggac gagaacggca
      301 aaagctcgat cgacggcatt tatgccggcg gtgatatcgt gctcggcgcg gccaccgtga
      361 tcctcgccat gggcgaaggc cgccgcgccg ccgccgcgat caatgcgttt ctggctgata
      421 aaacctgatc cgtccgatcc gtccgatctg tctgattctt cttcatgacc cactcgaccc
      481 ccagacgcta cacgatatcc gaagaggtgg cgcacagcgc gattcatgcg ctgggcagcc
      541 acttgggcgc ggccatgctg gcactgatgg tggtgttcgg ggttcagagc ggcgagcagg
      601 tggcgtggaa agtggtcagc gctgcgctgt tcggcttctc catcatcctc ctctacaccg
      661 tctcgtcggc ctaccatgcg atcacgcacc cgcgcaccaa acaggtgctg aaggtgtgcg
      721 accacatggc gatctacatc ctgatcgcgg gctcctacac gcccttcaca ctggtcacgt
      781 tacggcctga atatccggtt atcgcctgga cgatcttcgg cattgtctgg ggactgacga
      841 tcatgggcat cgttttcaag gtcttcttca ccgggcggtt gcggctggtc tcgaccctcg
      901 cctatgtcgg catgggctgg atcatcgtct ttgcgatcaa gcccctgatg gccacgttgc
      961 cgaccgccgg tttactctgg ctgctgatcg gcggcctgtg ttatacccta ggcgtgatct
     1021 tctatctatg gcgcctgatg ccttttcatc atgcggtgtg gcacctctgc gtgctcgccg
     1081 gcacgctctg ccattttttt gcggtgctgt tttgtgtgat gatgtgacgc gactcacggg
     1141 cgcacaacac cttcacttcg gcttcccttc cgaaatgagc gctcctatct tcgagcggat
     1201 ttttaagagc ttctccggat cgcgttcata atcttccacc gagcgcaccg cttcggtaag
     1261 gacagcccgg gttctgtctt caccgctacg cgccatcgcg agcttcgtcc attcgcggtc
     1321 ttcgactccg tcgcgaacca tcgcgaagcg gatggagggc agtacatgct ccgcgccggg
     1381 atagagcaga acgccaccgc cgctcgaata tcggctgcgc ggcgtgtacg tcggccaatc
     1441 ggggaagaaa gtgtcgctct catcgagaca cttcgcattg ttcggccagt agttgacgtg
     1501 ccagtagagg aagccgtctg ccccgcgcca atccgtcatg aatccgacga gcatgcggcc
     1561 ctcaagcggg actgactcga gcgaatcgaa gttcggtccg gggtaaaatg gcgcgactgt
     1621 cgaataccac cacaccttcc ttcccttttc gcgcatccgg gcggagaatt cgggctggta
     1681 ggacctgatg ttcgggcaac accaatcagc atcataggcg tatttctcgt ccgccttccc
     1741 gttgaccgca aactcatagg cgcgtgcaac agaaaggagc ggcacatctg gaaaatcgcg
     1801 tttgtacttg cggtagatat cggcgatcca tgggaagcaa tcactttcgc gctcgtcaaa
     1861 accatagacg taggcgtact tgagaagtcc ggcttttctg tactcctcga cgtatggttt
     1921 caggcgctgc gcgaaggcct cgtaaaattt aggatccgca tacgtttcag cggtctcctc
     1981 gtagacaatc ttcacgttcg gatctttcgg ttccgaaaca acgttgagga tgttgaagag
     2041 attgagtccg cgcttgacgg cgtacttgag gtgctcgaca tccggcggcg ttgtgcgcga
     2101 gatattgtcc ggattaagcc tgtgatccag catcacgtcc caactctgtc gcttcatctt
     2161 ttcaaaacaa tccggatact gcgcccgcgt gaaggaatcc atcaccgtga acgagttgag
     2221 cgttccgaaa gtacgcggca gggcgaacgg acagactttg atcgaaagcg gaatgctggc
     2281 catcgtatcg ccattttccc tgacctcgac gattccgcag taaaggccgg gtgtcgcgtc
     2341 cggcgcggca tagactgtaa gccacagccc ctgagtcgcg ttctctcgga ccttgaacgg
     2401 tgccgctggc aggagcggat ccggaatcca gcgctctgcg ggatcaggcg agccggggtg
     2461 tatgagcccc tcgtgtccgc gcgttacgta accgacgcgt tcccacttga tttcgccgcg
     2521 gaactccctt cccgatttgt ccgtgagcgt gccgattttc aatgtgccac tcttccaagc
     2581 accgccttta cctgcggaaa cgagtatctg tgccgattcc cgttcgttac cggcgagctc
     2641 cagatcaatt cggcggttct tctccgtcat ttctgctgta gacggaaacg tgagcggtgt
     2701 caccttacgc atcgaacttg cggtccacac gaccgctttc ccggcgggga aatctctcgc
     2761 cacacgcggg gcacctatgg acgcgggacg tacataatcg cgttccgtca ccttgcgtcc
     2821 gtccagccgg atttcgtgta ctgcctcgcc atcggaatac ggaaagtccg agcgcacatt
     2881 ctccacgatg atttcgccac cgggggcgct ttcgcggcgt tcgagtttga gattgcggaa
     2941 ttccgccttg ccaacgccgt ggcggagtaa tgcgtagaac ttcacttcct tgaccggctt
     3001 ctccggaacg aacacgccgg ccgcgcgccg ccaggtatgg gtgccgcctg gaaaatcggc
     3061 ggcggcgtcc caataccagg tgccgtcggc gaatccgaca tcaaggaata tcccgtaatc
     3121 tgccgccttc gcgttttcgg acctgctcca gccgctgaaa aggatcggct tcgtatccgg
     3181 ctcgggatag gtgaaggtct tccaaagtcc gcctgtgaac ggctccgacg gctttttcgg
     3241 aaaagacgcc agataaagga cgccggtttc gcggttggtt ccctcggggc cgttcgcctc
     3301 gtcgcgcgcc tgataaaact ttccgtcgat ttccaccgcg ccaacccatg caccgatgct
     3361 gagccgctcg aagtcggaaa aatctttgga aaaacgcagg agcccgatct ctgggtcgtg
     3421 ccgatcggga tagatgccca cgagaaattc gtcatgcata aaattgatcg tctggattcc
     3481 gagcatcgtg tagccggtgt ccagatcaat gcgccggtcg aatttaaagt ccttcgtgta
     3541 gcggtagacg tagtttttga cgtggttcgg cggcagcccc ccgaccacgt agaaacatcc
     3601 gtccgcgaac gtgatacctc cgcagccgtg tacgacctcg ggaatgtccc attcgccgca
     3661 acggctgaga tcggacgctt tgaacgccag caccctgttg agggcgccgg tctccttgtt
     3721 gaactcaccg ccgttgaaag caacatagac gacgccatcc accacacaaa ggtcgccgta
     3781 atggagcttg tcgattgcgg acaccgcgac acgcctgccc aacttgtctg atttcaccac
     3841 ccggtcgctg aatgaccagt agaggaactg tccatcggaa tcgaccccct gcagatgcca
     3901 cttgtaacgg ccttcgcaga tgatcttgga cggcagatcc gccaccgtgt ccggacacca
     3961 tgtggacttg cggccgagaa cgccatcgga attaactgcg gatgccgtcg cgacaataat
     4021 cgccgacaat aacaacagag acaaacgaat gcgtggtttc atgttacaat tcgcaatttc
     4081 ctcgaacggt aaagccgtcc agagcactct ctgtcaccgt ttatttgaac aagagtgtaa
     4141 accccttgcg cttgagccag aggaaatctt cgacagcaag cgttcccggc gtgatacgca
     4201 gttcgtcgat ccagccatcg aagccgacat caacggcacc tgcgccgagc atcaggttca
     4261 tttctttgaa gcggcacatc atgttaccgg tgaaggtgta cgaaccgata ctctggtaat
     4321 cgcgataaac cgtaaccagc gtatcatcgc cgtctgcggc gaaggtcacc gcgacatggt
     4381 gccaagcacc atccccgaac gcgccgccga acgtgtgcga ttgtccggtt tgggtgtcgg
     4441 tgtcgacggc aactgccaga ttgccggagg catccgcaaa ggaaagcgcc catgagatat
     4501 cgcccgcctc gttctcggac gcgcggttga gccgcacgat tcccgccccc gcaaccgggg
     4561 ccgccgactt gaggaagaac tcgaccgtca cgtccgcttg gtcggcaacg aggtcatgtc
     4621 ccggataggt cagcatgcta ccattgaagc ggatcgaccc cgcattcgca gtgcgaacgg
     4681 gttcgccatg tttcccgtcg aaaatcgtcc gcgccggacg gtctgagtcc aaaacaggcg
     4741 ccacccctcc cgttgtgaac gccgccgccg cgggtgtcgc gaagaagtca gcgtaaggcg
     4801 taagcgcgaa ggtgtcgtca tagctcgccc agccgagcac ggcaccacac tcgaccgccc
     4861 gggatgtcag gaactggtag ggtttcaaga cgccgcgcgt gatgcgcagg ttgtcgtagt
     4921 gcatgttgag cacgtaacgt tcatcatagt aagatccgcc gagcatcaac tctcggcggt
     4981 agagatacca catactcccg acatcgggga acacgatgtt atccgccccg agacgccaga
     5041 caccgtcgag atagagatcg gcgcggttcg ccgtcttatc gtaggagagt gccagatggt
     5101 gccactggtc atcgcgcacg ttccggggga tgaactgcgg atgattcacc cagttacccg
     5161 tgagttgggt ggatccgaca aaggcggaga gcataccctt ttcccaagtg gcggcgttgc
     5221 cggggatcag aatctggaat cctcccagca tgcagaaaag atgcgggccg ccagaagacg
     5281 aagcgatgtc gccagcagag ggcagcttga aggagacctc gatcgtcacg tcgtcgccaa
     5341 aaaccatgtc gcgcgtgatc gccttaggca ggacgacgct gtcagcccag tcgttggtcg
     5401 tcgcatcagc cgaggtgagg cacgagacag cgttggtgac gaagttcgtg cccgcaaggc
     5461 ttgacctgac caacgccgtg gcgacatcgc ccacgagctg caccggcaca gaagaggtca
     5521 cggacgattt cgtgtaggtc gtcgcgaagt attccgccca gacggatgac ggccatgact
     5581 tgttcctata ggctgggggc gcattggtga tctccttcgc aaagtccgtg tagacggtat
     5641 atgttgtgcc accatcgaaa tcgagcagga aaagagtttc cggcgtcgac gcggctgaag
     5701 gtgccgcaat cggccttgcg cggagaaatt caccgactcc cagcaccctg tcggaaatgc
     5761 ggagttcgtc tagcgtgtgt tcgataatgc cataataaca ctggatgtga ggccccaggg
     5821 ccagcggctg ggcgttatcg tagcaaatat cgcccgtgta ggttttctgc atgcaccaag
     5881 tctggatctg atagtccaca taaaggctga acgtcttgga atcgtcatcg atcgtaatcg
     5941 cgatatgatg ccacttgccg tcattcacat gcgaataggc atcgtaaacc gtgtagcgcg
     6001 taccattcgc gtcgatttca gcgtagacaa gctcgttctt atacatgacg ttccaggtga
     6061 aaacactatt gttcgaactg cgctgggcga ggagataccg gtcgacgctg tgcgtcccgg
     6121 cgggaatctt cacgaagaac tcaatcgtca tcgacttgag gtgcaacgcc gcatcgttgt
     6181 cgatcagcac cgtcgagccg gccgactcgt gattgcttgt atcggcagac gcgaagaaaa
     6241 gcgcaccgtt gttctcgtaa atctcgccac tcacaggatc aacgaccttc aggtcactgt
     6301 tgaaggtccc cgtataaagc ggcatcagcg attccggcgt cgtaagctgc tgcgcgccgc
     6361 tccacagaat cggcgtctgc actttggcgt gacccggcaa tttcgtcgga tcgacgctgt
     6421 tgacgatcac cgagtcggcg gtcgtgcgcg ttccggacgg ggcttcttca aaccggtacc
     6481 aggcaaccgt tttcgcggtt gcctgcaaac cgacaaccat cgtcaaagat aacattaacg
     6541 tcaccatcac gtttttctta gccatgtcgt gtcctccttt tattcgagcg tttcccaaag
     6601 tcctcacaca tccatcagta tcagtcaccc acaccgaacg agtagaacct gcagcgcccc
     6661 tcgttgccgt tgataccggc gtagaacgac gccggcagat catctgaacg gaacccgaac
     6721 tggcaactgc cgcaggtgac cgtcatctgg cgagtcttgc caccgccgac gacgttacgc
     6781 tccttgatcg tcaccgtgac cgtcaacggc gtgcgcggcg cgaacggagt cacgacaaac
     6841 gcgggtttgg tgatgacaaa ccccgtctcg gtccacttgt atcgccagac attgatgccg
     6901 ccgtcgaaca ggatgatttc gaaatgatcg cgcagttcct tcccggtcgg tccatcaaca
     6961 agttctggag caattagaat acccggggcg aacctgtcgt caaagctcat ccgcgccgaa
     7021 atactgacgg cttcggagaa acggttcgtc atcaccatgg cggaatacca gtccgctatc
     7081 ttcgtccccg gacattcgtt caggatacag tcctccgcct gttcgaagcc atgacgataa
     7141 ttccagcgtg ggttcgcgac attccaccac gccttctgat cccatttgcc ggctgcgaaa
     7201 cagatgtcct ccgcagcgaa caatccaaac gctgctaccg aaaccgctat taaaacaagt
     7261 gtcttcatgt tgttacttaa tggtgattgt gaaacctgaa tggttcaggc tcatacgaat
     7321 cacgccgcct tcggccgtga aggtgccgcg caggttagtt acgcctgtac ccgtcacacg
     7381 ccagcgtgaa acgtccggca ctgtccccgt gtagtgcgcg acgacgacat cctgcggata
     7441 gggacggaca agcggattct cgctccggcc aagatcgaca tccgttctgc cgacgaacgc
     7501 acaattttca aagagcggtg tcgcggcaac cgtgccgttc tcggcaatcg tcgcgatgat
     7561 cgtcgtgtcc cggagcgtgc cgccggaaac ggtgccggaa ccgccgaaga cggcattcgt
     7621 gaccataccc gccacggaaa gattgaccgt gccctcctcg atgtcacacg ttcccgtgaa
     7681 ggcatacgaa ccgttgccga acttgagcgt acccgcgccg gtctttgcga tgccaccgtc
     7741 gccaatcacc ttcgaatctg tcgcaaacat cgtgccggac ggcgtgttaa gcttgatacc
     7801 gccgccggtc gtccggataa agagattcgt ctcggcgccg accgtcgacg tgatcgtgat
     7861 atcggaggca ccgtaatccc acgtcgcgtt gtcgaagacg agcgtaaacg gcttgcgcaa
     7921 gaaaggaagt ccgtcgccag tagtgtaaac gccattcatg tttacagact ttgccctgaa
     7981 cacggatccg ttacggaaac ggacctcacc gctcggatag atgctgttgt agccatagct
     8041 gaatacgagt tcgaagccgc cagacggggt ggtgtcgatc atgcagttgt cgaagtcgtg
     8101 gtcagccgcg ccgtaatagt gcagcttccc gctcgaagtc gaaatgagca gggtcgagtc
     8161 cttagcccgc acaaccgtct tttcggatcc gtttgctccg ccgttaaggt agagcgcata
     8221 cgttgtcgtt atcgtcgcgt tcgtcatcgc caacgtcggt acggctcccg agcggcagcg
     8281 gttaccgagc gtgaagttgt gcgtcttgac cgaagcgccg ttcacgagcg tgagcttcgg
     8341 ataaagcgtg tttgcattcc actccgaacc gttgcagtaa ccgatgccga acgtcgtttc
     8401 caacggcgtt atcagttgca cgccgtcaag cgtcaactcg ggagatgtcg ttgctgtcgt
     8461 gcatttctcg ccgatgatca ggttgcccct tgtggtgagg gtcttcggcg ttccgtccgc
     8521 cgatctgata acgaggccgc cctcgacgat catgatcgga tgggccttcg tgactgtcgc
     8581 ggtcccgtcc gcattgaacg aaacctcttc cgtgtagtcc tgtccgccgg tcagattcga
     8641 tgtgtcccac acgatgttct gcgtaagcgg aaccgtaagc gtgcctgcgc cgcgtttcac
     8701 gagcttgccg ccagccgtga cgtcaagcgt agccgtcgca ttcgtccgtg cgtcgatgac
     8761 caccatggcc gaggtatcaa acgtgacggg acgcgcaagg ttaatgttgc cgtcggcatc
     8821 aaaagccaaa gtgccacctt ggacacgggc ggcccacgcc gattccgccg gaccgagagc
     8881 cccgtccgaa ccgacaaggg tcgtgccggc cgccagtctg acgtccagat cgaaggtatt
     8941 gtcaccgcgc agagccagca tgccgtcacc cgtcttcagg atgccgccat gtgaaatgtc
     9001 gccgtccaga ttgactgcgg cacccgccgc aatcggggct tcgacaagcg tattgaacgt
     9061 catgggaacc gagacggtat tcacgcccgc cgcccccacg atctgcgctg cgccctgcgc
     9121 accggcgatt tcgaccgagc cggagccggt gagcgaataa ccatccgctc cgaacgaaag
     9181 cgccccggcg actgcggcgg aatcgaccgt cacggtctta cccgcggccg tggacgtaaa
     9241 cgcggccttc ttgtccgcat cgggcatccc gtttggcgtc cagtttccgg aatttgccca
     9301 caacccgctg cccgtccaag tcaacgtcgc atccgcgccg atcggctcgg cgttgtcctc
     9361 aatggtcacg gtgacactcg tcacgccgcc ttccgtcgcc gtttgaaact gcgcgtgcgt
     9421 gccgtccgca agcgccacgt cacaaaacgc acgctggagc tcggacacag atgtgtcatc
     9481 gagcgtccct tggacaacga agactgtctg cgccgtgtac atagccggtg cggacgagaa
     9541 gacgagccgg agtcccttga acgacgcatt cccctgaaca gtgatcgtat cgcccggatc
     9601 aagcgcgatc gtcgcgttcg ttaccgcgag cgaggcgagc gttgctgagg tcgggtcgcc
     9661 gacaagcgag aacgtgccgc ccgtcacgac gcgcagggcg gacgagaccg tggcgcccgc
     9721 gtcaagttgc caagtaccgt tcgagacgac gaggttactc acggaacact cgcctgcgta
     9781 agtcagcgtg cctcggcctg tcttcacgaa aagacccgat tcgccgggct tgttgatgag
     9841 attctgtgcg atcttgacat cgtggccgtt cgagtcgaag acaagcccgc gcgacccgaa
     9901 ctccgccaca tcgagtccct ccatcgacgc gtaggtcgag ggattcatgt tctggcggaa
     9961 cgtgccgcca ttcgcagaaa agcgtgagtg gccgcttccg ccttggctct gcgatccctt
    10021 gccgccaagg atacgcgcgg cctcgaaaac gccgccatca agcacgtatg ccgcacggtt
    10081 catgatgtcg cggtcattcc aatgaccgcc agccgcgccg gaataggcca tcagcgcgac
    10141 acgcgaatat ttggcgtcgt tcaacgtatt gacagacact cttccgccaa cctgatggat
    10201 aaacgaggtt gtcgtcaacg ccgtggtttc aaagccgaca ttgatccgcg gggcaatgat
    10261 ctcgcctccg taaacatgca cctcgccgtg accgccgccg ccgacggcaa ggccgtcggt
    10321 cgcgccggcg gcccaactgt tcgtacccat gcgaatctca accttgccgc tgtcatgcac
    10381 gttgagcctt ccccagcccg taccatacgt gtccgcatat tttccgacat acaactggca
    10441 ataggttgac cgcggcgtac ggaacgtgcc cgacacgaca tcgatcacgc ccgtaccgcg
    10501 ccagccgatg tgttgtcctt gactgctccc gctcttaaca tagtcgacga ccgcgccgcc
    10561 cttgacggtc atcttgccgt ccgagttgct ctgaaagccg atttggggat aaccgaatga
    10621 ataggttccg ccgtcaaagg tcatttcggc aggttcgccg ttgttccccg cagcggagat
    10681 gatgagtgtg ttggtggcgg taagggtgca gttttcaaac gtgacggacg aattccaaga
    10741 gttcaccatt tccccggcaa tgtcgatcgt gcagtccttg aagacgttcg tcgccgtatg
    10801 gtagaaaccg acggccccgc cgcatacgaa tgtcgtattc gtcgcggcga ggacaagcgc
    10861 actttctgaa tagtaatagt tgtcgcggcc gaactgcaac gcgccatgga cggtcagttc
    10921 ggcgttatcg aagagcattg aacccggctt ggtataaccg gcataaagtt tcgggaacac
    10981 ggctttcgcg ccgttgacga gttcgaaacg gaacggctga ctgccgttgc tcatcaggat
    11041 ctcacgcagg gcatacgccg ttcctgcggg gttgcaggca ttgagcgtgc cggagtccat
    11101 acggaacgtg acggcacctg cggtgtcctg agagattttg aagcgcgcgt cgtcgaaaag
    11161 gatcggcgcg gccttgatct tgtccgcagc gtccaacttc gagtcatcga gtttgaacgc
    11221 ttccttctca tccgcgtcaa gaatcttgaa cggtgcgcca acgtagtagc cgttggccga
    11281 atacggcatc aatagcttgt acccccggcc atcgaacgtc agcgacgctg cggttgtctt
    11341 cgctacggac gagcggaagt agagcgaggc gtagtcgttc gtgctgtcgg cggacatcgt
    11401 gagcgtgatg tcgcccgtcg gcaacaccat tacggcctcg cccgccacgg gcttcgcgcc
    11461 gccggaccac gtcgaatccg cattccagtc cgcgctcgca gccggatctg caagcgtgcc
    11521 accggcctga actgcgaacg caaaccctaa agtcaaaacc ccaaccaatc cttgaatgat
    11581 tttactcatg gccgtttcct ttgcttagta atcgtttctg ccgaatcacc gcagcaaatc
    11641 gcacacatac tcttcggcct tgtaggtgta gattccgttc gcaaaaacag cgttcccgca
    11701 cataccgcgg agctggccgt aggaagccgg cagggtgaag gtcgtgtcat ggtttttgcc
    11761 aaggccgacg acgatcacgc gccaacgtcc cttcccgtcg gaaagcgccg cgacgcggac
    11821 ggggtctttc gcgtccttgt gcggcacgtc aatgatcttc tcgccgctca ggataaacgg
    11881 ctccagatcc ttgaacacct ttgcgatgtt gcacatcgct gtccaccggc ggtcgaccca
    11941 ctccgggatc ggtccgcgat agagatcaaa gagcgagtag aagaagaacc cggtcacacc
    12001 gtccgcggcg ttcaggagcg cgacagcgag cagctcgttc tctgtcggct cgcggcccaa
    12061 ctttcggtag agcaccgcgt tcgtgcgtgc ctcttggttc tgatttaccc agttgtagca
    12121 ctggggggcc caccagatcg ccgcagggcc gcattcctgc atccgcgtga cgcgattgtg
    12181 cgtgtccatc attccgccgc cgccgaggcg gatcggatac gcatcgccgc cttggatgtc
    12241 cgccgcaggc agatacgggg ccgctcgcat gatgttacag atgttgacca ttcgtccggg
    12301 gcagacctcg ttgagcatcc gccgtgttgc ggcgagtccg ggcacgacgc tttccgcgcc
    12361 ctcgtccatc gtatagaacc cgatgagcga agggaacttt tccagatacg cggcgagcgc
    12421 acggcttttg tcaatggcgt tcgttgttgc gctgccgtgg ccgaatgcgt ccgtcagaac
    12481 gccgattccg tacttttcgc agagccggta atacgtctcg caacgcggtc ccgtcctgag
    12541 cgaatagctc tggtagtcga tcatcgcatt gaagccggcc tcgtgcagct tcttgagctc
    12601 ggcctcgagt ttcgccgcgt cgtatttctt cggatctgcg aggtcggtgt agaagccgaa
    12661 cggcatgaac ggctctccgt tccggaggac gacgccgttc tcttggacga acaggtgctt
    12721 cgacctgtcg ggtgccgggc aaatctcgat ctccagcgtc tttgtgccga gctcctgccg
    12781 gtgaatggta tcgtagatcg tcgccgtcag tgttgccggc cctctgtagt cgagctttcc
    12841 gaacgccgcc gtcatcacgc ccgtctcgtc gggagcaagc acacatcgtg caagtggaga
    12901 cgcggcggca gaggcgccat cgccacgcaa gagcttcagc tcgtaaacgt ccttgccgtc
    12961 cttttcaagg aaatcaccga agaaccagct atggcaacgg atgcgtccct cttcacagta
    13021 gatcttgttg tggacgggcc atgtgttaca aaaataccag accgggacat cttcccgcag
    13081 cgagatgttg tcgatgtcga tccaatcgtc ctctgtatag gtgtttttcc cggggcaatg
    13141 cgcgatgaag tgatagtcga ttttatccga cccctctttt gcgacaaacg tcacccaaaa
    13201 cgtcttccat ccgtcggcca gcggcttcga agatgcgccc cacgcgccat agccgctcgc
    13261 atacttacgc gaatctttcc agaatgtgtc gacggcgatc tgcgccgagg ccttgccgtg
    13321 cgtatggacg tccgccgaaa agatgtagcg cttccccttc gcaagcgcca ctcgctgctg
    13381 gaaccggtgc gtacgtccgt tcgccagcgg acggatgcgg agtccgccgt tgccgttgaa
    13441 gccaaacttg ccgtactcga cattggcgtt ttcccggccg aacttcgtca ggtcatcgaa
    13501 ctccgcgttg tcaaacgccc tgagctcggt cggcgcgccg aactcgcgct tcgaataacc
    13561 ctcaaactca cccccaccgg cagagatgcc aaagacgacc cacatcgtca caccaaacac
    13621 gaggcgcttc atgcccgaaa tgataccaga ttcttccctc aagataagcg gattttagtg
    13681 gttacctgat ttcatcccct tcgtataatg ggcggacatc acaggaagca gtgtgtttcc
    13741 ggtctaccct agaggacaat gacggagtta ataaggtctg ggagaaactc cgccggatgg
    13801 gcgcgtcgag tgatcttcat atgctcgcga cccgtgggca ctgttttcag tttaaagcgg
    13861 cacccggcac cggcagcggg acctgacttg atcaggtgtg ggacttcctt gtccgcaagg
    13921 ggttcgtgaa gtgaaatctg aaaacaggag agtggacatg ataaaggcgg caagaagtaa
    13981 gacgtcaacc cccaacgctc aaccctcaac ttgaaagttg agcgttgaga gctgaacgtt
    14041 tattacttcg tcttgatgcc cgttccagcg gcctggtcaa tatacagacc aatgaagaag
    14101 aacgggaagc ccagatacat ctctttgggc ggct
//
LOCUS       DAOSRB010000057        14085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4395_length_14085_cov_6.393086, whole genome shotgun sequence.
ACCESSION   DAOSRB010000057 DAOSRB010000000
VERSION     DAOSRB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14085
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4395_length_14085_cov_6.393086"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /gene="xseA"
                     /locus_tag="PLZ74_04670"
     CDS             complement(<1..1096)
                     /gene="xseA"
                     /locus_tag="PLZ74_04670"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOR97649.1"
                     /translation="MALPEKRALTVSELTRRIRQTLEDEIGSVWVEGEISNCKRYSSG
                     HTYFVLKDEKAQLNAVLFAGTQALLDPALRLEDGLRVRVFGEITVFESRGQYQLKVRK
                     VEEAGQGDLMRRFEELKRRLQAEGLFEDARKRPLPRLPQRIGVVTSPTGAVIHDILSV
                     LGRRFPNVQVRLAPVKVQGEGAAEEIAEAVAQFNRMCGSGSDWPADLLIVGRGGGSLE
                     DLWAFNEECVVRAVAESVIPVISAVGHNVDYTLCDFAADLRAPTPSVAAELAVAPKVE
                     LEAEVRRQARALRQALESRLAEYAARLERLRRASCLRQPRRMLENPAQRLDSLSLRLD
                     HGIRRQAQTLRQRIAAAVGGLRMRPERMLGQ"
     gene            complement(1107..1892)
                     /locus_tag="PLZ74_04675"
     CDS             complement(1107..1892)
                     /locus_tag="PLZ74_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOR97650.1"
                     /translation="MKILLVGDIVGGPGRRIFREVVRKLRGEHAVQAVVVNGENAAAG
                     NGITRALAEELFAAGADVITLGDHVWGQKDLEPTIAGEKRLLRPLNFPAGTPGQGSVT
                     VQSTIGSFTVVNLLGRVFMNPMDCPFRAMDAVLKTLPSQIPVLVDFHAEATSEKIAMG
                     HYLDGRVAAVFGTHTHVQTNDAVILPKGTAYITDLGMTGPAVSVIGREIAPVLKKFTT
                     GIPAKFEIARGPCVLEGAILELDRVSGRAVSLTPYRYREPAAG"
     gene            complement(1937..3514)
                     /gene="rny"
                     /locus_tag="PLZ74_04680"
     CDS             complement(1937..3514)
                     /gene="rny"
                     /locus_tag="PLZ74_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOR97651.1"
                     /translation="MTQFTWYDVSDATVGLLFGLAGVLGGYALRGLIGRWQADAVEKQ
                     AYLKLDEADKEVRNRIKEADIQARTEVVRAREEFEKSTKSRRKELQDIEDRLTFREEN
                     MDKKAALLEKKDQTLAQKQEELQQRDEALRQRKQEADRRYAEAEQRLQKLAGMTHDEA
                     RRDIQQRAEQEVRSEAGGMIRRVQEEARETADREAARIVTMAIQRYALSHASESMTST
                     VPLPSDDIKGRIIGREGRNIRTLEAVTGVNMLIDDTPEAVVISGFDPVRREIARQALE
                     QLVADGRIHPARIEEVVQSVTENMEKTIYEAGEAAAYEAQVQGVPAEILRCIGRLKFR
                     TSFSQNVLRHAIEVAHLMGMMAGELGLDPLIARRIGLFHDIGKALDHDAQGAHALIGA
                     EFLKRNGEDPVVVNGVAAHHEDVPSEGLYGVLCSAADAISSSRPGARVETVGVYVQRL
                     EKLEAIANRFEGVKKTFAVQAGREVRVMVDPGQISDQDAMVMARDISQQISATMQFPG
                     QIKVVVVRESRCVEYAK"
     gene            3784..3860
                     /locus_tag="PLZ74_04685"
     tRNA            3784..3860
                     /locus_tag="PLZ74_04685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3818..3820,aa:Arg,seq:tct)
     gene            4097..7726
                     /locus_tag="PLZ74_04690"
     CDS             4097..7726
                     /locus_tag="PLZ74_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97652.1"
                     /translation="MTMTRKMVAAVVAAVAALALRAEYTDGLLWSFSAKQGELDGTFD
                     ATKFVDAVTEGAATPFTLTFNYDDIAARPYFTNMTVRSGLQGKSFQQTCLYMPQPTNG
                     TPGVDFVTKCACATVPHAKLAQTNVTFFIRYSWDGKYYNQKDFGGQYAYNYTITIYDN
                     ARDYGSSKGWTFYFSGVDSYTTTHPYVLRSLWVPYTANDCWLGWNQAAGSDWIDLAVT
                     LRNTDEGETEFKYYVCRAGETSITTYSHITSGKGGIPLPGSGDGKLFGAHGTTLAGGT
                     TPDAFRGAISKVALWDRALSAEEINFVFRDAGMPLILNNENLVLNEDRRVSYVIINGD
                     CTINGPGKLILSTPQGVTVSNGTATLSCPVEFDSGAEAVRMTVDATSKLVQTGVWSGA
                     TPIKLIGPGSDYATHWFELRGTNTFTGNLIFDRAKVYLYGGASVGTADGSTSWTVSKG
                     YNGSLVYFMGGIFAENFSAAHNNNYTGWWQSTRFAEDTTNIFTGALNLGNAYYYVAEA
                     GSRTFFDGTVAYNDYTPSVNAGAEITFTTNCSYQGSSFYPGGGGITHIYSQRKGGYGT
                     TFEYRVQSGMTWKMYGTNLINGTVGRGGEIHGTLDLNGFDQFMGACRSAQATGVVTSG
                     TPATIIFDQSNYADVVNANVFAGAVNLKKQGANNFGLSGASTSTGTLQIAEGTVTMAP
                     TGSWAGLIDVADGTTLAFASGRTLTAGTMIAVADGGTIALDAQLVADAGQISVDGQPL
                     AVGKYTSANGFISGDGSIIVSAAMGDPIVVEADMTIDEDVLVPSVTFKGQYRISGTGS
                     ISVRGGGIVIDDGAENKKGYAIDVPLMLDGNIAGSGYLTLMKGGSFLSATEITNVNLI
                     VSGTLDAANGMTFVQTPPANDRAELILSNAVINAGTAQITLATEDRYYALRSLANTTN
                     AVNCGLLHGSSSNGRFLTENGAKTDFNCPVNNLSYFCPSGGGEMWFNGKVSAWKLSAS
                     EGAIHLAAAGNVFSFDGTAVPQYATAVAIGKNHTLFCNIAGALNDRRTAIQPNGTLVL
                     TGDQFVGATIGSGTVTSPTGATYDFEQRMFTSSGEIQVTGPTTNTIVFADSVNVTKRG
                     TLMVYFGGVSTSTGELQVVEGAVGFSDAGCWRTCPTVRVGANGRLVLGGMRRFDWTTD
                     LYLETGAKLEIPGGTGVNFANLYIDGVKQPPGYYKKTNLPAFIEGDGTLCTTGEGFGT
                     VLRLR"
     gene            7731..9743
                     /locus_tag="PLZ74_04695"
     CDS             7731..9743
                     /locus_tag="PLZ74_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97653.1"
                     /translation="MKRIFAVVPLAAVMCAGAKTITVDYAGTGEYRTIGEAMKVVTKG
                     DIVLVKPGVYREEVRIPVGGASKDRPTILRSAVRGKAVVKGSEIWKNPWTKHGDGTKN
                     VWTSPFDKSFFPVWTNSPYMRTISIADRDRSRVARPVTNAVVDAETYLPRTIGQIFVD
                     GALLTEVTTIRELEGVPDTWMVSMDGEAIWLHPAENRAEPNACEIEWSVRNRCIGANR
                     RGLADVVVDGFTVEHCANQGPFPQIGAVDVRSGVRWIVENNTVRYAKSAGITCGSETW
                     GDWKNGEPWKDQRFPDMPDADQRLMFTRDCEIRYNAVTDCGVHGIAAWNPGGAKIYCN
                     VIERNNFGDYPEFGWEETAGIKIHGAPAVVANNVIRDNEAYGVWFDTGFTDVRCTGNF
                     IASNRRGGIMFETCVGHALVDNNVIIGNRPDENQYYNGDGVYSHNGSGVTVAHNFIGF
                     NGGAGVRFRTTYGKNGGMPDGVHTSSNRYVNNIFCDNAKGDLMIASENKFCRGTVSDY
                     NLFLRTGGQTSHIGTLSPFCYTYYNQGENSWSNLWQQCCSAGLDMGYGAWMEFGHPME
                     LSVWQKVRGLDLHSKVVRLTQGKAAQIAYRELSALFKFPMELEDVRVPAIDGPLTDYL
                     GAEYPAPGTLVRPGPFQKFKCAGKPGYYQALVPQVLKAAPRNLKGE"
     gene            9743..11125
                     /locus_tag="PLZ74_04700"
     CDS             9743..11125
                     /locus_tag="PLZ74_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97654.1"
                     /translation="MRILRTCGTLVAVCFAVMMTEAANRVARFENGMGFVADVEKALK
                     SGADYDAFVFRVKSGDDKDEFSRAVEQIRQARPATSVIAVYPREDAAWRAIIDRLIIA
                     PYCDGPEKENAYVDYIVSVNWGVDRKHPKYLALSERANRRTQRLHDSKWGVFNHFLGH
                     GCRTAEEWNAKVNGFDVKALGERLSACGARFYFITIMQGRRWMLAPNATYDRICGTKP
                     GEACAKRDLVLELSEELERRGIDLYLYYTGDGPYLDPELGPKMGLVSPRIGGVDRRFV
                     ENWAAVLEEYAVRYGKRVKGWWVDGCYEELLKYNDDLLDVYTAAIRKGSGEAIVAYNN
                     GVREYFTAYGRSADFTAGEFNDFYAVPKGRFVNGQQAFALIPLGDWTQGGDGAGWCSP
                     GMKISSEKLVNYVRTVNERGGVVAIDVRINPDSSWDADQFEALKALKPISAKASPSMP
                     GACSDHGGKR"
     gene            11197..13680
                     /locus_tag="PLZ74_04705"
     CDS             11197..13680
                     /locus_tag="PLZ74_04705"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97655.1"
                     /translation="MKEVARTFLAAGIVLFGSSVLLGDSAEEALALAQRTFAYVAKSV
                     PAAQLETCRRELESNAAWLASTTNDPPAYRAGVERAIRSLRRRILFLHPDLQFAKLLA
                     VQRGLPYTWDLHMVDQYVGRFSRPGPGLVVLENWKDAPKKTVLLADKLPAGTVLNPDL
                     NWDADRILFAFCDHTAAPPAGFDPSQYGFHADATPNDWVSQADPTHPIYGGKFRPGEE
                     VKLAHRRYFIWECAADGSWLRQITGGPGDKMETRDGRQTVLIEDIDPCYLPDGGFAFS
                     STRTQNFGRCHWGRYVPSFLLYRGDLTPLGAPAGSHNIRPLSLGEANEWEPAVLNDGL
                     IAFTRWDYINRNAVWHQSLWTIKPDGTGVAHLYGNYSERVCVGTEIKPLIGSPVCVAT
                     ASAHHSFTLGSLFLLDPRRGEDGDAPITRLTPECPFPESEGWKLPMTYCNPQPVNDTL
                     FFCARSDEPLAYPKDHPRHHRSSCGATWQAPNSFGIWLVDTLGGRELIYQDPAISTFN
                     PIPLVKRPKPPALASLLPPADKAPPYGVCYVENVYDSRVDLPKGSVKALRVNRLINMP
                     ACRRDTLHQGRDLEIYREYLGEVPVTEDGSAAFRIPAGEPIQLQALDKDGMAILTMRS
                     FIHAQKGEVQGCLGCHEQKSQTGRIASAPPNRTVLDPVKSVDLGYPGAFSYLRSVAPI
                     FEKHCLKCHTGGEGKPPSLAGLEGQRLMVAKKHVSFASSYFETHYSKPYDYFAAVSPL
                     TKRLKAGHGNVKLSPEEWRKLILWMDYNVPEFSHGGGYSWNRLETAGPNPPAHDVQGT
                     CGRGTNCMCRSCWVREGGYNKPRNLTTLR"
BASE COUNT         2690 a         4284 c         4290 g         2821 t
ORIGIN      
        1 cttgtcccag catgcgttcc ggccgcatcc gcaggcctcc gaccgctgcg gcgatacgtt
       61 gccgcagtgt ctgcgcctgc cgccggatgc cgtggtccag acgcaacgac agcgaatcca
      121 gccgctgcgc cggattttcg agcatgcgcc ggggctgccg caaacaggaa gcgcgccgga
      181 gccgctccaa tcgcgccgcg tactccgcga ggcggctctc cagcgcctgt cgtagcgcgc
      241 gtgcctgtcg ccgcacctcg gcctccaact ccacttttgg cgccaccgcc agttccgctg
      301 caaccgaagg cgtgggcgcg cgcaagtccg ccgcaaaatc gcacagcgtg taatccacat
      361 tgtggccgac tgccgagatc accgggatca ccgattccgc cacggcgcgc accacacact
      421 cttcgttgaa ggcccacagg tcctccagac tgcctccacc ccgcccgacg atcagcaggt
      481 ccgccggcca gtcgcttccg gaaccgcaca tccggttgaa ttgtgccacg gcttccgcga
      541 tctcttctgc ggcgccctcc ccctgaacct tgaccggcgc gagccgcacc tgcacgttcg
      601 ggaaacgccg ccccaagaca ctcaggatat catggatcac cgcgccggtc ggcgaggtca
      661 ccacgccgat ccgctgcggc agacgcggta gcggccgttt ccgcgcgtcc tcgaaaagcc
      721 cttcggcctg taaccgtcgt ttgagttcct cgaaacgccg catcaggtcg ccctgtcccg
      781 cctcctcgac cttacggact ttgagctggt attggccgcg gctctcaaac acggtgatct
      841 cgccgaacac gcgcacgcgc agtccgtctt ccagacgcaa cgcgggatcg agcagcgcct
      901 gggtgcccgc gaagagcacg gcattcaact gcgccttctc atccttcagc acgaagtacg
      961 tgtgaccaga gctgtagcgc ttacagttcg agatttcacc ctcgacccac acgctgccaa
     1021 tctcgtcctc cagcgtctgc cggattcggc gcgtgagttc ggagaccgtc aacgctcttt
     1081 tttcaggcaa cgccatgtcc ggttcctcat cccgccgctg gctcccgata ccggtacggc
     1141 gtcagggaga ccgccctgcc gcttacccgg tcgagttcca gaatcgcgcc ctccagtacg
     1201 cagggtccgc gggcgatctc aaacttcgcc gggatgccgg tcgtgaattt tttcagcacc
     1261 ggcgcaatct cgcggccgat cacggaaacc gcggggccgg tcatccccag atccgtgatg
     1321 taggccgtgc ccttgggcag gatcaccgcg tcattggtct gcacatgcgt gtgtgttccg
     1381 aagaccgctg ccacacggcc atcgagatag tggcccatgg cgattttctc ggaggtggct
     1441 tcggcatgga aatccacaag caccggtatc tgggacggca gtgtcttcag caccgcgtcc
     1501 atggcgcgga acgggcagtc catgggattc atgaacacgc gcccgaggag gttcaccacg
     1561 gtgaacgagc cgatggtgga ctgcaccgtc acgcttccct gacctggcgt gccggctggg
     1621 aaattcaggg gccgcagcag ccgcttttca cccgcgatgg tcggttccag atccttttgg
     1681 ccccacacgt ggtccccgag cgtgatcacg tccgcgccgg ccgcgaacaa ctcctcggcc
     1741 agcgcgcgcg tgatgccgtt tcctgccgcc gcgttctctc cgttcaccac caccgcctga
     1801 accgcatgct ctccgcgcag ttttcgcacc acctcgcgga atattcgccg tcccggcccg
     1861 cccacgatat cacccaccag cagaatcttc atcgcccgcc cctttccgat attcccgcgc
     1921 tgtccgccgc ctgcggtcac ttcgcatatt ccacacagcg cgattcgcgc accactacca
     1981 ctttgatctg tccggggaac tgcatcgtgg cggaaatctg ctggctgatg tcgcgcgcca
     2041 tcaccatcgc gtcctgatcg ctgatctgcc ccggatcgac catcacgcgc acttcgcgcc
     2101 cggcttgcac ggcgaaggtc tttttgaccc cctcgaaccg gttggcgatg gcctccagct
     2161 tttcgagacg ctgcacatac acgcccacgg tttcgacccg tgcgccgggc cgcgaactgg
     2221 agattgcgtc cgccgccgaa cagagcacgc cgtagagtcc ctcggagggc acgtcctcgt
     2281 ggtgcgcggc cacaccgttg accaccacgg gatcctcgcc gttgcgtttc aagaactccg
     2341 cgccgatcaa ggcgtgcgcg ccctgcgcgt cgtgatccag cgccttgccg atgtcgtgga
     2401 agaggccgat gcgccgtgcg atcagggggt ccagccccaa ctcgcccgcc atcatgccca
     2461 tcaggtgggc aacctcgatg gcgtgccgca acacgttctg cgagaaactc gttcggaact
     2521 tgaggcgtcc gatacagcgc aggatctccg ccggaacgcc ttgaacctga gcctcgtacg
     2581 ctgcggcctc gccagcctcg taaatcgtct tctccatatt ttcggtcacg ctctgaacca
     2641 cctcttcgat gcgcgccgga tggatgcgcc cgtcggcgac caactgctcc agcgcttggc
     2701 gcgcgatctc gcgccgcacg ggatcgaacc cggagatcac caccgcctcg ggtgtgtcgt
     2761 cgatcagcat gttgacgccg gtcacggctt ccagcgtgcg gatattgcgg ccctcgcgcc
     2821 cgatgatgcg tcccttgatg tcgtcgctcg gcagcggcac cgtgctggtc atggactcgc
     2881 tggcgtggga gagcgcatag cgttgaatcg ccatggtgac gatgcgtgcc gcctcgcggt
     2941 ctgcggtttc ccgggcttct tcctgcaccc gccggatcat cccgcccgct tcgctgcgca
     3001 cctcctgttc ggcccgttgc tggatgtcgc gccgggcctc atcatgcgtc atgcccgcca
     3061 atttctgcag ccgttgctcc gcctcggcat accgccgatc cgcttcctgc ttacgttgcc
     3121 gcaacgcctc gtcccgctgt tgcaactcct cctgcttctg ggcgagcgtc tgatcctttt
     3181 tttcgagcag ggccgccttc ttgtccatgt tctcttcgcg gaaggtcagg cgatcctcga
     3241 tatcctgcaa ctccttacgt cgggacttgg tgcttttctc gaactcctcg cgcgccctca
     3301 ccacctccgt gcgggcctgg atatccgctt ctttgatccg gttccggacc tccttgtccg
     3361 cctcgtccaa cttgagatac gcctgttttt cgaccgcatc cgcctgccag cgtccgatca
     3421 ggccgcgcaa ggcatacccg cccagcacac cggccagacc gaaaagcaga ccgaccgtgg
     3481 cgtcactgac gtcgtaccac gtgaattgcg tcatgaaacc tcccaacgcg ttaccgcata
     3541 tgattgtgtc acatccgtgt ctggacgtcc cggccgaccg gacacaggtt caaatgggag
     3601 atatcttatc cgattcccgt aagcggggca acctttgagc aagcgaagtc ggaagcccgt
     3661 ccgttttctc cgcttgatcc ccgtggaagc cttgtgtacc attccgttcc gtaacgcgta
     3721 tccctatcgg ttacccgtgc gggacccgat tttccacgcg ttttccggcc tgctttttca
     3781 gatgcccctg tagctcagtt ggatagagca acggatttct aatccgtggg tcgcaggttc
     3841 aagtcctgcc aggggcgcca tttcaaaacc cccgtaaaaa cggtcagggc cacgcgatga
     3901 tgcactccgg ttggccggga aggtgcgtga cgatgcgcac ggccacgtcc ggcgtgccca
     3961 ccgtactgca tcgtgccata tcgcgcgccc tcgcacaccc cctgtcacga accgattgct
     4021 gcggttgacg tggtttcgtt tgtgttcacc tagggagaag tgatagaata tgcgcatgat
     4081 ttgcgaaagg acgattatga caatgacgag aaagatggtt gccgccgttg ttgcggcggt
     4141 tgcggctttg gcacttcgtg ccgagtatac cgacggcctt ctgtggtcgt tttcggcgaa
     4201 acagggcgag ctggacggta ccttcgacgc gacgaagttt gttgacgcgg tgacagaggg
     4261 agcggccact ccctttacgc taaccttcaa ctacgacgac atcgcggccc gtccgtactt
     4321 caccaacatg actgtgcgga gcggccttca gggcaagagt ttccagcaga cctgcctcta
     4381 catgccgcag ccgacgaatg gcacgcccgg tgtcgacttc gtcaccaagt gcgcgtgcgc
     4441 aactgtgccg cacgcgaagc tcgcgcagac caacgtcacg ttcttcatcc gttacagttg
     4501 ggacggcaaa tactacaacc aaaaggactt cggcgggcaa tacgcctaca actacaccat
     4561 tacgatttac gacaacgcgc gcgattacgg ttcgagtaag gggtggacgt tctatttcag
     4621 cggcgtggac tcctatacca ccacccaccc gtatgttttg cgttcgctgt gggttccgta
     4681 tacagcgaac gactgctggc ttggttggaa ccaggctgcc ggttcggact ggattgatct
     4741 tgcggtgacg ctgcgcaata ctgatgaagg tgagaccgag ttcaaatact atgtctgccg
     4801 ggctggcgaa acctcgatta cgacctactc ccacatcacc agcgggaagg gcggaatccc
     4861 gcttccgggc agcggagacg gcaaactctt cggcgcgcac ggaacgacgc ttgccggcgg
     4921 caccacgccc gacgccttcc gcggtgcgat ctcaaaggtc gctctctggg accgtgcgct
     4981 ttcggcggaa gagatcaact ttgtgttccg tgatgcgggc atgccgctca tcctgaacaa
     5041 cgagaatctc gtgctgaacg aagaccgtcg ggtgtcgtac gtgatcatca atggcgactg
     5101 tacgatcaac gggcctggga agctcatcct cagcacgccc cagggcgtca ccgtgagcaa
     5161 cggtacggcg acgttgagct gtccggtgga attcgacagc ggcgcggagg cggtgaggat
     5221 gacggtggat gccacctcga agcttgttca gaccggagtg tggagcggtg cgacgccgat
     5281 caagctcatt ggccctggca gcgattacgc cacgcactgg tttgagcttc gcggcacgaa
     5341 caccttcacc ggcaacctca tcttcgatcg cgcgaaggtc tatctttacg gcggggcatc
     5401 ggtgggtacg gcggatggca gcacgagctg gaccgtcagc aagggataca atggcagtct
     5461 cgtctatttc atgggcggca tatttgccga aaacttctct gcggcacaca acaacaacta
     5521 taccggttgg tggcagtcga cgcggtttgc ggaagatacg acgaacattt tcacgggtgc
     5581 gctgaatctc ggcaatgcct attactacgt ggccgaagcc ggttcgcgga cgttcttcga
     5641 cgggacggtc gcctacaacg actatacgcc gtcggtcaac gctggcgcgg agatcacgtt
     5701 caccacgaac tgttcctatc agggctcgtc attctatccg gggggcggcg gcatcaccca
     5761 tatctattct cagcgtaagg gaggttacgg aacgaccttc gagtacaggg tccagagcgg
     5821 gatgacttgg aagatgtacg gcacgaatct tatcaacggc acggtgggac gcggcggcga
     5881 gatccatggt acgctcgacc tcaacggctt cgatcagttc atgggggcat gccgttccgc
     5941 ccaggccacc ggtgttgtca cttcgggcac gccggccacg atcattttcg accagtccaa
     6001 ttacgcggac gtcgtcaacg ccaatgtctt cgctggcgcg gtgaacctga agaagcaagg
     6061 cgcgaacaac ttcggtttga gcggggcctc gacgtccacc ggtacgttgc agattgccga
     6121 gggaacggtg accatggcgc cgacaggcag ttgggcgggg ttgatcgatg tcgccgatgg
     6181 gacgacgctc gcttttgcca gcggccgcac gcttacggct ggcacgatga tcgccgttgc
     6241 ggacggtggc accattgcgc tggatgcgca gcttgtcgcc gatgcgggcc agatctctgt
     6301 ggatggccag ccgcttgccg tgggcaagta cacgtccgca aacggtttta tttccggcga
     6361 cggctcgatc attgtgagcg cagcgatggg cgatccgatc gtggtcgagg cggacatgac
     6421 gattgacgaa gacgtgctcg tgccgtccgt caccttcaaa ggacagtaca ggatttccgg
     6481 tacgggttcc attagcgttc ggggcggcgg cattgtcatc gacgatggag cggagaacaa
     6541 aaaggggtac gcgatcgacg tgccgctgat gctcgacggc aacatcgccg gcagcggata
     6601 tcttacgttg atgaagggcg gttccttcct ttcggcgacg gagatcacca acgtgaactt
     6661 gatcgtctcg ggaacgcttg acgccgcgaa tgggatgacg ttcgtgcaga cgccgccggc
     6721 gaacgaccgc gccgaactca tcctttcgaa cgcggtgatc aatgccggaa cggcgcagat
     6781 tactctggcg acggaggacc gctactatgc gctccgttcg ttggcgaata caaccaacgc
     6841 cgtcaattgc ggcttgcttc atgggagttc ctctaatgga cggtttctca ccgagaatgg
     6901 agcaaaaaca gatttcaact gtccggtcaa caacctttcg tatttctgtc cgagcggtgg
     6961 cggcgagatg tggttcaacg gcaaggtctc ggcctggaag ttgtctgctt ccgaaggcgc
     7021 cattcatcta gcggctgcag ggaatgtctt ctctttcgac ggtacggcgg tgccgcagta
     7081 tgcgaccgcg gttgccatcg gcaagaatca cacgcttttc tgcaacatcg cgggggcact
     7141 caacgaccgt cgcacggcga ttcagccgaa tggcacgctc gtgttgacgg gggatcagtt
     7201 cgttggggcg acgatcggct cgggcacggt cacttcgccg acgggtgcga cctacgactt
     7261 cgagcagcgc atgtttacga gttcaggcga aattcaggtt acggggccga ccacgaacac
     7321 gatcgtcttc gccgattcgg tgaacgtgac gaagcggggc acgctcatgg tctatttcgg
     7381 cggtgtttcg acctccactg gcgaactgca ggtggtcgag ggcgcggtcg ggttcagtga
     7441 cgcgggctgc tggaggacgt gtccgaccgt gcgcgtcggc gccaatggcc gtctcgtttt
     7501 gggagggatg cgtcggttcg actggactac cgacctctat ctcgaaacag gggccaagct
     7561 cgagatcccc ggaggaacgg gcgtcaattt cgcgaatctc tacatcgatg gcgtcaagca
     7621 gccgccgggt tactacaaga agaccaacct tccggcgttc atcgaaggcg acggcacgct
     7681 ctgcacgacg ggcgagggtt tcggtacggt attgaggctc aggtaaaaca atgaaaagaa
     7741 tttttgcggt ggtaccgctg gcggccgtga tgtgcgccgg ggcgaagacg attacggtcg
     7801 attacgctgg caccggggaa taccgcacga tcggcgaagc gatgaaggtg gtcacgaagg
     7861 gcgacatcgt gctggtgaag cccggggtct accgcgagga ggtgcgcatt cccgtgggcg
     7921 gcgcgtcgaa ggaccgtccg accatcctgc gcagcgccgt gcgcggcaag gccgtcgtga
     7981 agggaagcga aatctggaag aacccatgga cgaagcacgg tgacgggacg aagaacgttt
     8041 ggacctcgcc gttcgacaag tcgttctttc ccgtgtggac caactcgcct tacatgcgca
     8101 cgatctcgat tgccgatcgt gaccgcagcc gcgtggcgcg tcccgtgacg aacgcggtgg
     8161 tggatgcgga gacctatctg ccgcggacga tcgggcagat cttcgtcgac ggcgcgctcc
     8221 tgacggaagt gacgacgatc cgggaactcg agggcgttcc cgacacctgg atggtgtcga
     8281 tggacggcga ggcgatctgg ttgcatccgg ccgagaaccg cgcggagccg aatgcctgtg
     8341 agatcgaatg gtctgtgcgt aaccgttgca tcggcgcgaa ccggcgcgga ctcgccgacg
     8401 tcgtggtgga cggtttcacg gtggagcatt gcgcgaacca ggggccgttc ccgcagatcg
     8461 gggcagtcga cgtccggtca ggcgtccgct ggattgtcga gaacaacacg gtgcgttacg
     8521 cgaagtccgc cggcatcacc tgtggttccg agacctgggg cgattggaag aacggtgagc
     8581 cctggaagga tcagcgtttc cctgacatgc cggacgcgga ccagcgactc atgttcacta
     8641 gggactgtga gattcgctac aacgctgtga cggactgcgg cgtgcacggg atcgccgctt
     8701 ggaatccggg cggtgcgaag atttactgca acgtgatcga gcgcaacaac ttcggggact
     8761 atcccgagtt cggctgggag gagacagcgg gcatcaagat ccacggggcg ccggccgtgg
     8821 tcgcgaacaa cgtcatccgc gacaacgagg cgtacggcgt ctggttcgac accggattca
     8881 ccgatgtccg ctgcacgggg aacttcatcg cgtccaaccg ccgcggcggc atcatgttcg
     8941 agacgtgtgt cggccacgcg cttgtcgaca acaacgtcat catcggcaac cgtccggatg
     9001 aaaaccaata ctacaatggg gatggcgttt actcgcacaa cggttccggc gttacggtcg
     9061 cccataattt tatcggcttc aatggtggcg cgggcgtcag gttccggacg acctacggca
     9121 agaacggcgg catgccggac ggggttcaca cgtcgtccaa tcgctacgtg aacaacatct
     9181 tctgcgacaa tgccaagggc gatctgatga tcgcgagcga gaacaagttc tgtcgtggca
     9241 cggtttcgga ttacaacctt ttcctgcgta ccggtggaca gaccagtcac atcggcaccc
     9301 tctcgccgtt ctgctacacc tactacaacc agggcgagaa cagttggagc aatctctggc
     9361 agcagtgctg ttcggcgggt ctcgacatgg ggtacggcgc atggatggaa ttcggccatc
     9421 cgatggaact ttcggtgtgg cagaaggtcc gcggactaga ccttcattcg aaagtggttc
     9481 gcctgaccca gggcaaggcc gcgcagatcg cctatcgtga gctttcggcg ctctttaagt
     9541 ttccgatgga acttgaagac gttcgcgtgc cggcgattga cggcccgctt accgactatc
     9601 tcggggcgga atatcccgca ccggggacgt tggtgcgtcc gggaccgttc cagaaattca
     9661 agtgcgctgg caagccgggt tattatcagg cgctggttcc gcaggtgttg aaagccgctc
     9721 cgagaaatct gaaaggagaa taatgagaat tctaaggaca tgtgggacgc tcgttgctgt
     9781 ctgttttgcg gtgatgatga cggaggccgc gaatcgggtg gcgcggttcg agaacggaat
     9841 ggggtttgtc gccgatgtcg aaaaggcgct taagagcgga gccgactacg atgcgttcgt
     9901 ctttcgggtg aagtccgggg atgacaagga tgagttttca agggcggtgg aacagattcg
     9961 ccaggcaagg ccagcgactt cggtgattgc cgtttatcct cgcgaggacg cggcgtggcg
    10021 tgcgatcatc gatcggttga tcatcgcgcc gtactgcgat ggaccggaga aggagaacgc
    10081 ctatgtcgac tacatcgtgt cggtgaactg gggcgttgac cggaaacatc cgaaatatct
    10141 ggcactgtca gaacgcgcca accgccgtac ccaacggctt cacgattcca aatggggagt
    10201 cttcaatcat tttctcggtc atgggtgcag gacggcggag gaatggaacg caaaggtgaa
    10261 tgggttcgac gtcaaggcgc tcggtgagcg gctcagtgcg tgtggcgcaa gattctattt
    10321 catcacgatc atgcagggcc gacgttggat gctcgcgccg aatgcgacgt atgaccgcat
    10381 ttgcggaacg aagcctggcg aggcatgtgc gaaacgcgat cttgtgttgg aactttccga
    10441 agaactcgaa aggcgtggca tcgaccttta cctttactat accggggatg gaccttatct
    10501 tgacccggaa ctcggcccga agatgggact cgtttcgccg cggatcggtg gtgtcgatcg
    10561 gcgatttgtc gagaactggg cagcggtgct tgaggagtat gccgttcgct acggcaagag
    10621 ggtgaaggga tggtgggtgg atggctgcta tgaggaactg ctcaagtaca atgacgacct
    10681 gcttgacgtg tacacggcgg cgatccggaa gggcagcggg gaggcgattg tggcgtacaa
    10741 caacggcgtg cgcgagtatt tcactgccta tgggcgttcg gcggatttca cggcagggga
    10801 attcaatgat ttttacgcag tgccgaaagg gcggttcgtc aatggccagc aggcgttcgc
    10861 cctcattccg ctcggggact ggactcaagg cggagacggg gccggttggt gcagtccggg
    10921 gatgaagata tcgtctgaaa agctggtgaa ctacgtgcgt accgtcaacg aacgcggcgg
    10981 tgtggtcgcg atcgacgtga ggatcaatcc cgacagcagc tgggatgctg atcagtttga
    11041 ggcgcttaag gcattgaaac ccattagcgc caaagccagc ccctccatgc ccggggcgtg
    11101 ttctgatcat ggcggcaagc gataggggag ggggagggtt gtcagagggt gccgaggggg
    11161 cggaacgcga tggtcttgaa aggagaaaca agaaccatga aagaggttgc acgcacattt
    11221 ctcgccgctg gcatcgtcct gttcggctct tcagtcctct tgggcgattc ggcggaagaa
    11281 gcactcgcgc ttgcgcaacg tacgttcgct tatgtggcaa agtccgtccc ggctgcgcaa
    11341 ctggagacct gccgacgcga actggagagt aatgccgcgt ggctcgcctc caccacgaac
    11401 gacccacccg cgtatcgcgc gggtgtggag cgggcgattc ggtcgcttcg ccgccggatt
    11461 ctctttctcc atcccgactt gcagttcgcg aagctcctcg ccgtccagcg cggtctgccc
    11521 tacacgtggg acctccacat ggtggatcag tacgtgggac gcttctcgcg tccaggccct
    11581 ggtcttgtgg tgctggagaa ttggaaggac gcaccgaaga aaaccgtgtt gctcgcagac
    11641 aagctccccg caggcacagt gctcaacccc gatctgaact gggacgccga ccgcatcctt
    11701 tttgcgttct gtgaccacac agcggcgccg cccgccggat tcgatccgag tcagtacggc
    11761 ttccatgcgg acgccacacc gaacgattgg gtgtcgcagg ccgatcccac gcacccgatc
    11821 tacggcggaa agttccgccc tggcgaggag gtgaagctcg ctcaccgccg ttacttcatc
    11881 tgggaatgcg ctgcggacgg ttcttggttg cgacagatca ctggcggccc tggggataag
    11941 atggaaacac gcgacggacg gcagactgtg ctgatcgagg acatcgaccc ttgctatctg
    12001 cccgatggcg gcttcgcctt ctcttccacg cgcacccaga actttggccg ctgtcactgg
    12061 gggcgctacg tgccgtcctt cctcctttac cgtggtgatc tcacacctct gggcgcaccg
    12121 gctggctcgc acaacatccg tccactctct ctcggcgagg cgaacgaatg ggaaccagcc
    12181 gtactgaatg atggcctcat cgcttttacg cgctgggact acatcaaccg taacgccgtg
    12241 tggcaccagt cgctctggac gatcaagcct gacggcacag gcgtggcgca tctttacggt
    12301 aactattccg agcgcgtctg cgtggggacg gagatcaaac ccctcatagg ttcgcccgtg
    12361 tgtgtggcca cggcctctgc ccaccactcc ttcaccttgg gatcgctctt cctgctcgat
    12421 ccccggcggg gcgaagacgg cgatgcgccg atcacgcgcc tcacgccgga gtgcccgttc
    12481 ccggaatcgg agggatggaa actgccgatg acatactgca atccgcagcc ggtgaacgac
    12541 acgctcttct tctgtgcgcg ttcggacgag ccgctcgctt atcccaaaga ccacccgcgc
    12601 caccaccgca gttcctgcgg tgctacttgg caggccccca actcgtttgg catctggctt
    12661 gtcgatacgc tcggcggacg cgaactcatt tatcaggatc ccgctatctc cacgttcaac
    12721 ccgattccgc tcgtgaaacg tccgaagccc cctgcgctcg cctcgctatt gcccccggcc
    12781 gacaaggcgc ctccgtacgg cgtctgctac gtggagaacg tgtacgattc gcgcgtggat
    12841 ctccccaaag gcagtgtgaa ggcgttgcgt gtgaatcgtc tcatcaacat gccggcctgc
    12901 cgtcgcgata cgcttcacca agggcgcgac cttgaaatct acagagagta cctcggtgaa
    12961 gtgcccgtga cagaggacgg ttcagcggca ttccgtattc ctgcgggaga gccgattcaa
    13021 ctgcaagcac ttgacaagga cggcatggcg atcctcacga tgcgcagttt tattcacgcg
    13081 caaaaaggcg aagtgcaggg gtgccttggc tgtcacgaac agaagtcgca aacggggcgc
    13141 attgcatctg cgccgccgaa ccgcaccgtc cttgatcccg tgaagagcgt ggatctgggt
    13201 tatcccggcg cattcagtta cctgcgtagt gtggcaccga tcttcgagaa acactgcctc
    13261 aaatgccaca cgggcggcga aggcaagccg ccatccctcg cagggcttga aggccagcgc
    13321 ctgatggtgg caaagaagca tgtgtcgttt gcaagttcgt acttcgagac gcactattcg
    13381 aagccctacg actatttcgc ggcagtgagt ccgctcacga agcgtctcaa ggctgggcat
    13441 ggcaacgtaa agctttcgcc tgaagaatgg cgcaagctca tcctctggat ggactacaac
    13501 gtgccggagt tctcacatgg cggcggctac agttggaacc ggcttgaaac cgctggcccc
    13561 aatccgcccg cgcacgacgt gcagggcacg tgcggacgcg gcaccaactg catgtgccga
    13621 agctgctggg ttcgcgaagg tggttacaac aagccacgta atttgacaac tctgcggtaa
    13681 gcgtgtacag cctcccgtac acatctgttc gccggacttg cgggcatcgg gaggggcaaa
    13741 cgtttgcttg cccgaggacg agcagacgct cgtccctccc ggtttgcgac atcgctatcg
    13801 ggatcgatag agagtgaaga gcggaaaacg ctcaacgtca aagttatagg taagaaataa
    13861 aaggtaagag gtaaaaagtc gggaacgcgg gcattcgccc gctggcaggg cggtttcccc
    13921 gaaaccgccg cggacgcctc agggaggcgt ccctaccggt tgaagaactt tcaccgcact
    13981 ccaaaatttt cgcagccctt gtttgtctca tgtcaaacca cgacccccga tactccgccg
    14041 ttgtattcct cgaagaatta tgctacgctc cgtttatagg aagag
//
LOCUS       DAOSRB010000058        14063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4406_length_14063_cov_6.255140, whole genome shotgun sequence.
ACCESSION   DAOSRB010000058 DAOSRB010000000
VERSION     DAOSRB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14063
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4406_length_14063_cov_6.255140"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1275
                     /locus_tag="PLZ74_04710"
     CDS             64..1275
                     /locus_tag="PLZ74_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97656.1"
                     /translation="MRKMIWFLFLLCAAALPWESACRAAEAPPVITVVKKGAEKNTVS
                     LSRLNASGPYGKLFVRTLTRDLELSGWFKVASSGAVQVQGTVADAGSGVQSACRVTWP
                     GKAFNWNRVSMGQAEVRRQAHQLADEMVRLITGEIGIAQSRIVFVNRRGRNNADLYLC
                     DADGQNLMQITHDNVAAVGPRWAPNSQDIYYTSFLKGYPAVYRIGVGRGERKTLAAFK
                     GLNTGAAISPDGSRAAIILSYQGNPELYVLHLTSGQLTRLTQTVHAAEASPCWSPDGR
                     QIVYVSDPAKTPQLYLVDPLSRQSRRLTYKGSENVNPDWSAKGKIVYATRRGGGGYQI
                     AVLDPRQGEASCELLTQPGGYEHPSWAPNSRHVVCSNRSTLYILDTLGDPAVRLINLA
                     GNWMSPDWSSR"
     gene            1297..1860
                     /locus_tag="PLZ74_04715"
     CDS             1297..1860
                     /locus_tag="PLZ74_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR97657.1"
                     /translation="MHIRSIVAVNVVLMGLLLFTDTGCRLPWQKGKGKGGSAGLGDPT
                     LLENADLAQVTDEFGHTLSGARFEDTLQPVQGVTFAPVYFAFDSYALPPTEISKIDQV
                     GQHLHQNAGHVLVVEGHCDERGSNEYNLSLGENRAQALRAHLVNQGISADRIQTRSYG
                     EEKPAVAGSGEEVWRLNRRGEFALFQK"
     gene            1888..2343
                     /locus_tag="PLZ74_04720"
     CDS             1888..2343
                     /locus_tag="PLZ74_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOR97658.1"
                     /translation="MSFFPSVAFLGGSVGTGEWIVLFVVVLIVVGPKRLPEIARKLGR
                     TMEMFRRAADEFKDQLLNMDREPAEATPPPETDAAYDPDGYGVPSDPAEPYDGTPYPD
                     TGEYADATDVPGEATPVSDGIPETDGSAGDAAAEPAASSKPEQPTEDVS"
     gene            2340..3152
                     /gene="tatC"
                     /locus_tag="PLZ74_04725"
     CDS             2340..3152
                     /gene="tatC"
                     /locus_tag="PLZ74_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOR97659.1"
                     /translation="MKVGRFSLPKFGQGDEYNDPPRPFLEHLIDLRDCLIHCAIAWVL
                     CEILIVPFAPRITNWLIAPAGVSKDMVQGLGWTAGFSILLKIMLWGGTALSLPFLLFF
                     IMRFVFPGLKRSERSIIIFCLFTSSLLFAIGVWMAYAATLEIAIGVLQKINAWVGIKV
                     DIVRLDEHVDIVIKTIIAFGLAFQLPLLLLVLGWIGLVPSEALRSRRRIAIVVIFVIA
                     MILTPPDPVSQILMAVPMCALYEACIWIIRLRELARGDEKDDEPSAPDNDAD"
     gene            3171..3761
                     /gene="hisB"
                     /locus_tag="PLZ74_04730"
     CDS             3171..3761
                     /gene="hisB"
                     /locus_tag="PLZ74_04730"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOR97660.1"
                     /translation="MARTAARTRTTRETDIAVTLNLDGSGVSRIETGVGFFDHMLELL
                     ARHALVDLEVRAKGDVHVDYHHTVEDVGLVLGACLNEALGERRGIRRYGFALLPMDEA
                     LCEVVIDLGGRPFLVFASPMKHMRVRDFEMQLLEEFFRAVTVEGRLNLHVRQVYGDET
                     HHVCEAIFKGFARALRMAVEPDPRETGIPSSKGTIA"
     gene            3758..4480
                     /gene="hisA"
                     /locus_tag="PLZ74_04735"
     CDS             3758..4480
                     /gene="hisA"
                     /locus_tag="PLZ74_04735"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011127638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOR97661.1"
                     /translation="MIILPAIDLKDGKCVRLRQGRAEEVTVYSDDPVAQAREWLAQGA
                     EQLHVVDLDGAFQGEPQHMEQIAQIVRAVGIPVEVGGGLRTDAQIEQMLGVGVARAIL
                     GTRAVESLDTLRALARRFGAAIAVGIDARDGFVQVRGWVETTKVRAVDLARRVEDAGV
                     RTIIYTDTATDGMLGGPNLTALREMCATVACRVIASGGVSAPEHVAALRDLGFPNLYG
                     AIVGKALYDGKTTLAAMRAAAC"
     gene            4489..5778
                     /locus_tag="PLZ74_04740"
     CDS             4489..5778
                     /locus_tag="PLZ74_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOR97662.1"
                     /translation="MLGKTKITRLKNGVSVVTSAIENAESIAMGIWIGVGGRHERRPL
                     AGVSHFLEHMLFKGTPTRSALAVSQAIEGRGGYLNAFTQEEATCYYARLPYEYLPQAF
                     DVLSDMYLHAAIREEDFRRERDVILEEIKMYQDLPQHVVQENLLEAMFRDHALGAPLS
                     GNPKSLGAMSRETLLRYKARAYTPGSTVFAFAGRLDHEACVARVERAVGGQPRRRGLD
                     FMRVDGRVPQRRLSLTRKEIAQTHAVIGFRIFGRHDDRRYALRVLNGVLGENMSSRLF
                     QSVRERRGLCYSINSGFQLFEETGLFTVSGGFDARRTIAALKRTAQEMRRLIDAPVGA
                     RELRRVKEYLQGTFRLGLEGVGNQMMFIGESLLNYGRVVCPEETLAGIAAVTADDVQR
                     VAEEVFEPSRLTLSLVVPNAQAESEDEWMSSLGALFA"
     gene            5806..8493
                     /locus_tag="PLZ74_04745"
     CDS             5806..8493
                     /locus_tag="PLZ74_04745"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR97663.1"
                     /translation="MRKAGQGSETPLRVPPDENWRTTDQDEINRRKRRAASEPMTILN
                     RTPAHPVFSNFNVTSRSSGQTYQVEVREISPRVGSCTCPDFRVNDLGTCKHLERVIRE
                     LACGEPLSDRIDVVPDRVMGRLLIERNLNRLPAALRPYFDITGLALPEFSPTEILEAC
                     ERVGPDVVRVSQDVEPWMARLARQTERIRLRRAYEHAVCSGHYPPQETLEPLLPYQRE
                     GMLHLAFTGRALLADEMGLGKAVQAVAAVALLYRLGEVRHAVAVVPEAHQREWEGHIR
                     RYTALSCETVAGDRAARRRAYEQKAAFTLVSYEQVLRDSRLFLDVLNPECVILDEAQR
                     IRNWNTRTAQTLKQIESRYLFVLTGAPLEQRLDELYTLMSLIDPEVLGPLFRFNREFY
                     TLDEDGRPTGYQNLHRLRERIRPYVLQRRRTEVETELPDRTERVVYVPMAPKQRDRYA
                     THETQATLLAAQSQQRALTRSETERLRREIAMMRMLCDTPYITDETSRVCPKLEELRN
                     AMESVLAMSGVKVLVCSEWERMLQLVRALCEQQGWGYAWHVGSVPQGQRKREIRRFRN
                     EPACRVFLATDAGCPAGSLSQASVVIQCDVPWTAARFEQRIARVCHSHQVRSVTVLNL
                     VAADSIEARLLEQLKQGRANGAAVGARVSLTQRLATLLPLTEATERTPVEHPSFEALR
                     ALDPELGFAAALAERMGEKLVACDVWHTEEGRPVFLLAAEGPLRVANLIADRLAADWF
                     AHVAAGPVLEVVAPGVSDVLRRFMDKGWLRMPHPVGARTLYQRGQGLSRPRMMPDDEQ
                     ARILELREQAGQAFRQARRLMRQLAFDAARAPLELSMVLLARAFARQHRLAEPRHARQ
                     TLTEPYAELWGDLLPVLREGLQENSPVALSRALMAKFGR"
     gene            complement(8536..9861)
                     /gene="aroA"
                     /locus_tag="PLZ74_04750"
     CDS             complement(8536..9861)
                     /gene="aroA"
                     /locus_tag="PLZ74_04750"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOR97664.1"
                     /translation="MQHSLTLSFSADRPLTGEAVLPGDKSLSHRAALFAALAEGVSVV
                     DRFLVSGVTRAMLNALTALGVAWRLEENRLTVPGVGLHGFTPPTAPLDCGNSATTIRM
                     LTGALAAAGTPCVLDGSEGLRKRPMDRITEPLRAMGVRISTPPNHCAPITLAARSPDV
                     PLRAQAYTLPVASAQVKSCLILAALAADGETVLREPGPSRDHTERLLASMGADIRVCG
                     PEVRVTPLARPLAPLALTLPGDISSAAFLLVAAALTPGSTILLRDVGLNPTRTGILDA
                     LREMGADLTVENPQTAAGEPVGDLRLTAAPLQAVRVEGERVVRMIDEFPVFAIAACFA
                     RGVTEVRDAEELRYKETDRIAVLCAELRALGARVEERRDGFMIHGGSLRGGVCEAHGD
                     HRLAMSLALAGLAAPEPVSVRHAEILNESFPDFVTQLRALGATVAEIRG"
     gene            complement(9878..13678)
                     /locus_tag="PLZ74_04755"
     CDS             complement(9878..13678)
                     /locus_tag="PLZ74_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOR97665.1"
                     /translation="MNRSFFLFALFPFFPYVSGAEVLPEAFPLDRQSVMTVAASVTAE
                     RFPNADRVLVDNRVLERYERDGTSVVWDDEYNKILTEKGRRDASSVRIPFNAHYGTAL
                     VYRAEVIKPDGRTVALDIASYARVVVESEQMGANIYDPNNKILNLSLPGLEIGDVCHL
                     ITCRKTVKVRMPDTWADYAVFEYDSPIVRLEYAISAPPELPFRHALLRAPVPGTVMKT
                     ERREAEGRTLHLWTVTNVPQMYPEPDMPPLHTQVQRLLLSTIPDWRTVSRWYWELCAP
                     ALAATNTAMRTRVDALTAGSASRDETIRRIFKFVSQEIRYMGITTEDTAPGYEPHPVS
                     LTFDNRYGVCRDKAALLVALLRMAGIDAYPVLIHAGAKMDPDVPMPFFNHAVTAVDNP
                     DGGYLLMDPTDENTRDLFPAYLCNRSYLVARPEGETLRVSDVYPVEKNLTRINTEGTM
                     DRNGSLLLKTRIRFEGINDNAYRSYLVRQKPEQRRNFFEAALKNRLPGAEVIECMIAP
                     EDLRDTETPLAVTLTTRVPEFPVRGEGLDLLAMPWLGTGLGYANFVIGQTGLDKRRYP
                     LETGITCGTEERIALHAAAGLGAPHTLPGAVHIDRAGVFFAMSQSVSNSVLSGSLTYH
                     LKTPEFSPSDYLELKSILKEIEFAGRKRPLFEAGDPNPPDHETLHDLTEAHLVSPHAW
                     TTTHTWSRRILTYAGKKSCSELKIPFNPVWQQIDLLAAVVSNANGIVHTVTPKEINQM
                     DAAWTGSAPRYPAGKLLVVNLPGVETGSVITVSTRFTQTNAPFYCHTHTFGGTAPVAE
                     RVYRLTYPDTLSPAIRLVNPGSVTMDSETRDGLVTHTWHAPQQPVTRTEELLPPWHFF
                     RPVLFVSFGDWSEYMRQLRRAAETAAETDREARRRAKERVRGLRDPRERLLAIRDEVL
                     RTIRPAGPAFFDLPAECLSPPDRTLADQYGHPADRALLLRAMLRAVGFDAELLFAGQD
                     TTCYPAFSQPMREIPQIEFYRHPLVAVKLRDGICYLNEGDQYSELGSSELDGAPALTL
                     KGRPVTIELDEPLRNRSHHAWTIDLDVSGAARITVSNRFFGTQAGTFRKQYREMLPEE
                     RRRHHLELVSKIARAAEPASALITETEGYPCLRTYTVTAARYATLEGRTLTLLIPEVA
                     GVLFPLRADSRVNPLFLGMRDRSDLTCEILLPPGYTRLPVLPESKHWILPNGLGTLDY
                     SVKHITRADGRVQISITRALVRQSAELPAELYPALLEYNRRLAHPATRTLVAERPTNL
                     YPTP"
BASE COUNT         2538 a         3960 c         4849 g         2716 t
ORIGIN      
        1 gcgatgtctg cgcttgctgt tggcgagggg acggggtata ctcgtaacac attgggagtt
       61 tttatgcgca agatgatttg gtttttgttt ctgttgtgcg ccgcggcgtt gccgtgggaa
      121 agcgcttgtc gggcggcgga ggcaccgccg gtgatcacgg ttgtgaagaa aggcgccgag
      181 aaaaacacgg tgtcgctgag ccgcctgaat gcgtccggac cttacggaaa actgttcgtc
      241 cggaccttga cgcgcgatct ggaattgtcc ggatggttca aagtcgcgtc gtccggtgcg
      301 gtgcaggtgc aggggacggt agcggacgcg ggcagcggtg tgcagagcgc gtgccgggtg
      361 acatggccgg ggaaggcgtt caactggaac cgggtgtcga tggggcaggc cgaggtgcgg
      421 cgtcaggccc accagttggc ggacgagatg gtacggctga tcacgggaga gatcggcatc
      481 gcgcaatcgc ggatcgtgtt cgtcaaccgg cggggacgca acaatgccga cctgtacctg
      541 tgcgatgcgg acgggcagaa tctgatgcag atcacgcacg ataatgttgc ggcggtcggc
      601 ccgcgctggg cacccaacag ccaggacatt tattacacga gctttctgaa ggggtatccg
      661 gcggtttacc gtatcggggt ggggcgcggg gaacgcaaaa cattggcggc gttcaaggga
      721 ctgaacaccg gggcggcaat ttcgccggac ggttcgcgtg ccgcgatcat cctttcgtat
      781 cagggcaacc ctgaactgta cgttctgcat cttacttcgg gccagctcac ccggttgacc
      841 caaacggtgc acgctgcgga ggccagcccc tgctggtcac cggacggccg ccagatcgtt
      901 tacgtgagcg acccggccaa gaccccgcaa ctgtatttgg ttgatccgct ttcacggcag
      961 tcgcgccgtc tgacctacaa agggtctgaa aacgtcaacc ccgactggag cgccaagggg
     1021 aagatcgttt acgcgacccg ccgcggtggg ggggggtatc agatcgcggt gctcgatccc
     1081 cggcagggcg aggcctcctg tgagttgctg acgcagcccg gcggatatga gcatccctct
     1141 tgggcaccga acagtcgcca tgtggtctgc tccaatcgtt ccactctcta cattcttgac
     1201 actctggggg atcctgcggt acgtttaatc aaccttgccg gaaactggat gtccccggat
     1261 tggtcgagtc gttaaagggt tcagaggagt acacgtatgc atattcgttc aatcgtcgcc
     1321 gtcaacgtcg tgctgatggg tctgttgttg ttcacggata ccggttgccg tttgccctgg
     1381 caaaagggca aaggcaaggg aggctccgca gggttgggtg accccactct gttggagaat
     1441 gccgatctcg cgcaggtgac cgacgaattc ggtcatacgc tgagcggagc gcgttttgag
     1501 gacacgctgc aaccggtgca gggcgtgacg ttcgcgccgg tctatttcgc gttcgatagt
     1561 tatgcgctgc cgcctacgga gatctccaag atcgatcagg tcggccagca tctgcatcag
     1621 aacgccggtc atgtgctggt ggttgagggg cactgcgacg agcgtggcag caacgagtac
     1681 aacctgtcgc tcggtgagaa ccgcgcacag gccctccgcg cgcatctcgt caatcagggc
     1741 atttcagccg accggatcca gactcgcagc tatggcgagg aaaagcctgc ggtcgccgga
     1801 tctggagaag aggtgtggcg cctgaatcgt cggggcgagt tcgcgctgtt ccagaaatag
     1861 ggcgttgcgg gtcgggggct tccgggcatg tcgttttttc ccagtgtggc gttcctcggc
     1921 ggctccgtgg gtacgggtga gtggattgtc ctgtttgtgg tcgtcctgat tgtggtcggc
     1981 cccaaacggc ttcccgagat cgcccgcaaa ttgggccgta cgatggagat gttccgtcgt
     2041 gcggccgacg aatttaaaga tcagttgctg aacatggacc gtgaaccggc ggaggcgacg
     2101 ccaccgcctg aaacggacgc tgcctatgac ccggacgggt acggggtgcc gtccgacccg
     2161 gcggaaccgt atgacgggac accgtatccg gacacgggcg agtatgccga tgcaacggat
     2221 gtgccggggg aggcgacacc ggtttcggac gggattccgg aaacggacgg gagcgccggc
     2281 gacgcggcgg cggaaccggc ggcttcgtct aaaccggaac agcccacgga ggacgtctca
     2341 tgaaggtcgg tcgtttctcg ctgcccaaat tcggacaggg ggatgagtat aatgacccgc
     2401 cgcgtccctt tctggagcat ctgattgatc tgcgcgattg tctgatccat tgcgccatcg
     2461 cttgggttct ctgtgagatc ctgattgtcc ctttcgcgcc ccggatcacg aactggctga
     2521 tcgcgccggc cggagtttcg aaagacatgg tgcaggggct aggctggacc gccggtttca
     2581 gcatcctgct gaaaatcatg ttgtggggcg gcacggccct tagcctgccc ttcctgctgt
     2641 tcttcatcat gcggttcgtc ttccccggac tcaaacgcag cgaacgttct atcatcatct
     2701 tttgcctttt cacctcctcg ctgctgttcg cgataggcgt gtggatggcc tatgccgcca
     2761 cgctggagat cgccatcggt gtgttgcaga agatcaatgc gtgggtcgga atcaaagtcg
     2821 atatcgtgcg cctcgacgag catgtggata tcgtgatcaa gaccatcatc gcgttcgggc
     2881 tggcttttca gttgcctttg ctgttgttgg tgctcggctg gatcggactg gtcccgtccg
     2941 aagcactccg ttcgcgacgc cgcatcgcca tcgtggtgat tttcgtgatc gcgatgatct
     3001 tgaccccgcc cgatccggtc tcgcagatct tgatggcggt gccgatgtgt gcgctgtacg
     3061 aggcctgcat ctggatcatc cgcctgcgtg aattggcgcg gggggacgag aaagacgatg
     3121 aaccctccgc gccggacaac gacgcggact gagaattcgg gaggcacgcc atggcacgta
     3181 cggcggcacg cacacgcacc acacgcgaaa ccgatatcgc ggtgacgctc aatctggacg
     3241 gcagcggcgt cagccgcatt gaaaccggtg tgggattttt cgaccacatg ctggaactcc
     3301 tcgcgcggca cgcgctcgtg gatcttgagg tccgcgccaa gggtgatgtg catgtggatt
     3361 accaccatac cgtggaggat gtggggctgg tgctcggcgc ctgtctcaat gaggcgctcg
     3421 gcgagcgtcg cggcatccgt cgttacgggt tcgcgctgtt gccgatggac gaggcgcttt
     3481 gcgaggtggt gatcgatctg ggcggacgac cgtttctggt ctttgccagc ccgatgaagc
     3541 acatgcgggt gcgtgatttc gagatgcaac tgctggagga gttcttccgc gccgtgacgg
     3601 tggagggacg cctgaacctg catgtgcggc aggtgtacgg cgatgagacg caccatgtgt
     3661 gcgaggcgat cttcaaaggg ttcgcgcgcg cgttgcgtat ggcggtcgag ccggatccgc
     3721 gcgaaaccgg tattccgtcc agcaagggga cgatcgcgtg atcattctgc cggccatcga
     3781 tttgaaagac gggaagtgcg tgcggttgcg ccaggggcgg gctgaggagg tgacggttta
     3841 ctccgacgat ccggtggcgc aggcgcggga gtggcttgcg caaggcgccg aacagttgca
     3901 tgtggtggat ctggacggcg cgtttcaggg cgagccccag cacatggaac agatcgcgca
     3961 gattgtccgc gccgtcggca ttccggttga ggtgggcggt ggtctgcgca cggatgcgca
     4021 gattgaacaa atgttggggg tcggggtcgc gcgcgcgatt ctcggcacgc gcgccgtgga
     4081 gagcctggac acactgcgcg cgctggcccg gcggttcggc gcggccatcg cggtggggat
     4141 tgacgcccgt gacggatttg tgcaggtccg gggatgggtg gagaccacga aggtccgtgc
     4201 ggtcgatctg gcgcggcggg tggaggatgc aggggtccgt acgatcatct ataccgacac
     4261 cgcgacggac ggcatgttgg gcggacccaa tctcacggcc ctgcgcgaaa tgtgtgcaac
     4321 ggtcgcgtgc cgtgtcatcg catcaggcgg tgtgagcgcg ccggaacacg ttgccgcact
     4381 ccgcgatctg ggattcccga acctttacgg ggcaatcgtg ggcaaggcgt tatatgacgg
     4441 caaaaccaca ctcgcagcaa tgcgggcagc ggcgtgctga aggggattat gctcggaaaa
     4501 acaaagatca cccgactgaa gaacggggtg tcggtggtca cgtccgccat tgaaaatgcg
     4561 gaaagtatcg caatgggcat ttggatcggc gtcggcgggc gccacgagcg ccgtcccttg
     4621 gccggcgtga gccattttct ggagcacatg ctgttcaaag gcacgccgac gcgttcggcg
     4681 ctggccgttt cgcaggcgat cgagggccgc ggcggctacc tgaacgcgtt cacgcaagag
     4741 gaggccacct gttactacgc gcgcctgccc tatgaatatc tgccgcaggc gttcgatgtg
     4801 ctgtcggaca tgtacctcca cgccgcgatc cgcgaggagg atttccggcg cgagagggat
     4861 gtgattttgg aggagatcaa aatgtatcag gatctcccgc agcatgtggt gcaagagaac
     4921 ttgctggagg cgatgtttcg cgatcatgcg ctcggagcgc cgctttccgg caatccgaaa
     4981 tcgttgggtg cgatgagccg cgagaccctg ctgcgttaca aggcacgggc ctacacgccg
     5041 ggttcgaccg tgttcgcctt tgcgggcagg ctcgaccatg aggcgtgtgt ggcacgggtc
     5101 gaacgggctg tcggcgggca gccgcgtcgg cgcgggttgg atttcatgcg tgtggacggc
     5161 agggtgcccc aaaggcggct ttcgctgacg cgcaaggaga tcgcccagac gcacgccgtg
     5221 atcggattcc gtattttcgg acgtcatgat gaccggcgtt acgccttgcg ggtgttgaac
     5281 ggtgtgctgg gcgaaaacat gagttcccgg ctgttccaga gtgtgcggga gcggcgcggc
     5341 ctctgttact ccatcaactc aggattccaa ctgtttgaag agaccggtct gtttaccgtc
     5401 agcggcggtt ttgacgcgcg gcgcacgatt gcggcgctga aacggacggc gcaagagatg
     5461 cggcgcctga tcgatgcgcc ggtcggcgcg cgcgaattgc gccgcgtgaa ggagtatctg
     5521 cagggcacct tccggctcgg attggagggt gtcgggaacc agatgatgtt tatcggcgaa
     5581 tcgttgttga attacggccg ggtcgtctgt ccggaggaaa cgctcgcggg gatcgcggcg
     5641 gttaccgcag acgatgtgca acgcgttgcg gaagaggtct ttgagccgtc gcgcctcacg
     5701 ttgtcgctgg tggtgccgaa cgcacaggcc gaaagcgaag atgagtggat gtcgagtctg
     5761 ggcgcgttgt ttgcctgacg gacggactgc ggaaaggggg cgcgtatgcg caaagcgggt
     5821 cagggttcgg aaacgcctct gcgcgtaccg ccggacgaga actggcgtac cacggatcag
     5881 gatgagatca accgccggaa acggcgtgcc gccagcgagc cgatgaccat cctgaatcgt
     5941 acgccggctc atcccgtgtt ctcgaatttc aacgtcacct cccggagcag cggtcagacc
     6001 tatcaggtcg aagtgcggga gatttcgccg cgagtcggtt cctgcacctg tccggatttc
     6061 cgggtcaacg acctcggcac ctgtaaacat ctggaaaggg taatacgcga gctggcgtgc
     6121 ggcgagcccc tgtccgaccg catcgatgtg gtgccggacc gggtcatggg gcgtctgctg
     6181 atcgaacgga acttgaaccg gctgccagcc gccctgcgcc cttatttcga tatcaccggc
     6241 ctggcgttgc ccgagttctc cccaacggag attctggagg catgcgaacg ggtcggtccg
     6301 gacgtggtgc gcgtttcgca ggatgtcgag ccctggatgg cacgtctggc acggcagacc
     6361 gaacgcatcc ggttgcgccg ggcttacgag catgcggtgt gttccggaca ctacccgccg
     6421 caagagactc tcgaaccgct cttgccctat cagcgcgagg gtatgctgca tctggccttc
     6481 acgggccgcg ccctgctggc ggatgagatg gggttgggta aggcggttca ggcggtcgca
     6541 gccgtggcgt tgttgtaccg gctgggcgag gtgcgccatg cggtggcggt cgtgccggag
     6601 gctcatcagc gggagtggga ggggcatatc cggcggtaca cggcgctttc gtgcgaaacg
     6661 gttgcggggg atcgtgcggc gcggcggcgt gcgtatgagc aaaaggccgc gttcacgctg
     6721 gtcagctatg agcaggtgtt gcgtgacagc cgtctctttc tggacgtgct gaaccccgag
     6781 tgcgtgattc tcgatgaggc gcagcggatc cggaattgga acacgcggac agcgcagacc
     6841 ctgaagcaga tcgagagccg gtaccttttc gtgttgaccg gtgcgccgct ggaacagcgt
     6901 ctcgacgaac tctacacact gatgtcgctc atcgatccgg aggtgctcgg gccgctcttc
     6961 cggttcaacc gcgagtttta cacgctggat gaggacgggc gtcccacggg ttaccagaat
     7021 ctgcaccggt tgcgggaacg tatccggccg tacgttttgc agcgtcgccg caccgaggtg
     7081 gagactgaac ttcccgaccg tacggaacgg gttgtgtacg ttccgatggc gccgaaacaa
     7141 cgcgaccggt acgcgacgca tgaaacccag gccacgcttc tggccgcgca gtcgcaacag
     7201 cgtgcgctga cccggtcgga gaccgagcgg ttacggcgtg agatcgccat gatgcgcatg
     7261 ctttgcgata cgccgtacat tacggatgag acgagccgtg tctgtcccaa actggaggaa
     7321 ctccgcaacg cgatggagag tgtcctggcc atgtccggag tcaaggtttt ggtctgttcc
     7381 gagtgggagc gcatgttgca actggtgcgc gcgttgtgcg aacagcaggg gtggggctat
     7441 gcgtggcatg tcggttccgt gcctcagggg cagcggaaac gcgaaatccg gcgcttccgc
     7501 aacgagcccg cctgccgtgt ctttctggcc acggatgcgg gctgtccggc gggaagccta
     7561 tcgcaggcga gtgtggtgat ccaatgtgat gtgccgtgga ccgcggcgcg gtttgaacaa
     7621 cgaatcgcgc gggtgtgtca ttcgcatcag gtgcgcagtg tcacggttct gaatctggtg
     7681 gcagccgaca gcatcgaagc gcgtttgctg gagcagctga agcagggacg cgccaacggt
     7741 gctgcggtcg gcgcgcgtgt ctccttgacg cagcgtctgg cgacgttact gcctttgacg
     7801 gaggcgacgg agcggacacc tgtggagcat ccgtcctttg aggcgttgcg ggcgctcgat
     7861 ccggaattgg ggtttgcggc cgcactcgcg gaacggatgg gtgagaaact ggtggcctgt
     7921 gacgtgtggc acacggaaga gggacgcccg gttttcctgt tggccgccga aggaccgctc
     7981 cgtgtggcca atctgatcgc tgatcggttg gctgccgact ggttcgcgca tgtggcggca
     8041 ggaccggttc ttgaggtggt ggcgcccggt gtcagcgacg tcctgcggcg gttcatggat
     8101 aaaggatggt tgcggatgcc gcatcccgtc ggcgcgcgca ctctctatca acgcgggcag
     8161 gggctctccc gacccagaat gatgccggat gacgagcagg cccggattct ggagttgcgc
     8221 gagcaggcgg gacaggcgtt tcgtcaagca cggcggctga tgaggcagct ggcgttcgat
     8281 gcggcacggg cgccgctgga gttgtccatg gtactgctgg cacgagcctt tgcccggcag
     8341 caccgtcttg ccgaaccgcg gcatgcgcgg cagacgctca ccgaaccgta cgcggagttg
     8401 tggggcgacc tgctgccagt tctgcgcgag gggcttcagg aaaactcgcc ggtggcattg
     8461 tcccgcgcgt tgatggcgaa attcggcagg taacaaaccg aaggcgccga ccggagcgcg
     8521 tggccacgga tcggatcagc cgcgtatttc cgccaccgta gcgcctaagg cacggagttg
     8581 cgtcacgaaa tccgggaacg attcgttcag aatctcggca tgccgcacgc ttaccggttc
     8641 aggcgcggcg aggcccgcca gcgccagaga catggccaga cggtgatcgc cgtgggcttc
     8701 acagacgcca ccccgcaggg atccgccatg gatcatgaac ccgtcgcggc gctcctcgac
     8761 ccgtgcgccg agcgcgcgca gttccgcgca cagcaccgca atccggtccg tctctttgta
     8821 gcgcagttct tcggcatcac gcacctcggt gacgccgcgc gcgaaacagg cggcgatcgc
     8881 gaagaccgga aactcgtcga tcatccgcac gacacgctca ccctccaccc gcacggcttg
     8941 gagcggcgcg gcggtcaggc gcaaatcgcc cacgggttcg cctgccgcag tctgcgggtt
     9001 ctccacggtc aggtcggctc ccatttcgcg taacgcatcc agaatgccgg tgcgcgtggg
     9061 gttgaggccc acgtcgcgga gcaggatggt cgagccgggg gtcagggcgg ccgcgaccag
     9121 caagaacgcg gcggaagaga tgtcgcccgg cagggtgagg gcgagcgggg ccaggggccg
     9181 ggcgaggggc gtgacccgca cttcaggacc gcaaacgcgg atgtccgcgc ccatggaggc
     9241 aaggagacgt tccgtgtggt cgcgggaggg accgggttcg cggagaaccg tttcgccgtc
     9301 cgcagcgagg gccgccagaa tcagacagga cttgacctgt gcgctcgcca cgggcagggt
     9361 atacgcttgg gcgcggagcg gcacgtcggg gctacgcgcg gcaagggtga tgggtgcgca
     9421 gtggttggga ggagtcgaga tgcgaacgcc catggcgcgg agcggttccg tgatgcggtc
     9481 catggggcgc ttgcgtaacc cctccgatcc gtcgagcaca cagggcgtgc ctgccgccgc
     9541 gagcgcgcca gtcagcatac ggatcgtggt ggccgagttg ccgcagtcaa gcggggcggt
     9601 tggcggtgtg aagccgtgga gacctacgcc gggaacggtc agccggtttt cctccagacg
     9661 ccacgccacg cccaaggcgg tgagtgcatt gagcatcgca cgcgtgacgc cggagacgag
     9721 gaatcgatcc accacgctga ccccttcggc cagggcagcg aagagtgctg cgcggtgcga
     9781 gagggatttg tcgccgggaa gaaccgcttc accggtcaag ggccggtctg cggaaaagga
     9841 aagtgtgagt gagtgttgca tcgcggatga taatccttta gggtgtgggg taaaggttgg
     9901 tgggccgttc ggccacgagg gtgcgcgttg cggggtgcgc gaggcgccgg ttgtactcca
     9961 gcaacgccgg gtaaagttcc gcagggagtt ccgcgctctg gcgcaccagc gcacgtgtga
    10021 tcgaaatctg tacacgtccg tcggcgcggg ttatgtgctt cacgctgtag tccagtgtgc
    10081 cgagaccgtt cggcaggatc cagtgcttag attcgggaag cacgggcagg cgcgtgtagc
    10141 ccggcggtaa aaggatttcg caagtcaggt cagatctgtc acgcatcccg agaaacagcg
    10201 ggttcacacg tgaatcggcg cgcaggggga agagaacccc cgccacctcg ggaatgagaa
    10261 gcgtcagtgt gcggccttcc agcgtggcgt accgcgcagc agtgaccgta taggtccgca
    10321 aacaggggta gccttcggtt tcggtgatca gcgcggaggc gggttccgct gcccgggcga
    10381 tcttgctgac gagttcgagg tgatggcggc gccgctcttc tggcagcatc tcgcgatact
    10441 gcttgcggaa ggtgccggcc tgagttccga aaaaccggtt gctgaccgtg atccgtgcgg
    10501 caccggatac atcgagatcg atcgtccacg catggtgcga acggttgcgt aacggttcgt
    10561 cgagttcgat cgtcacgggg cgtcctttga gcgtgagtgc cggcgcacca tcaagttctg
    10621 aactgccaag ttcgctgtac tgatcccctt cattgagata acagatcccg tcccggagtt
    10681 tcactgccac cagtggatgg cggtaaaact cgatttgggg gatctcccgc attggctgcg
    10741 aaaaggccgg atagcacgtt gtgtcttggc cggcgaagag caattcggcg tcgaatccca
    10801 cggcgcgcag catggcccgc agcagcaagg cgcggtcggc aggatggccg tattggtcgg
    10861 cgagcgtgcg gtcgggcggg gagaggcact ccgctggcag gtcgaaaaac gcggggcctg
    10921 cgggtcggat ggtacgcaac acctcgtcgc ggatcgcgag cagacgctcc cgcggatcgc
    10981 ggaggccccg gacccgctct ttcgcacggc ggcgcgcctc gcgatccgtt tcggcagccg
    11041 tctctgcagc gcgtcgcaac tgccgcatat actcggacca gtcgccgaag gagacaaaga
    11101 ggacgggacg gaaaaaatgc cagggcggga gcagttcctc cgtgcgggtg accggttgct
    11161 gcggggcgtg ccaggtatgg gtgaccagac cgtcccgggt ttccgaatcc atggtgacgg
    11221 aaccggggtt gaccaaccgg atggcggggc tgagcgtgtc gggataggtg agccggtaga
    11281 ccctctctgc aactggggcc gttccgccga aagtgtgcgt atgacagtaa aagggggcgt
    11341 tggtctgtgt gaaacgagtg ctcaccgtga tcacgctgcc ggtttccaca cccggcagat
    11401 tcaccacgag cagtttgcct gcggggtatc gtggtgccga tccggtccag gccgcgtcca
    11461 tctgattgat ctctttgggc gtcacggtgt gcacgatccc gttagcgttg gaaaccacgg
    11521 cggcaagcag gtcaatctgt tgccagacgg gattgaaggg gattttcagt tccgaacagc
    11581 tcttcttgcc cgcataggtg aggattcgtc gggaccaagt gtgggtggtg gtccaagcgt
    11641 gcggcgaaac cagatgcgct tcggtgagat cgtggagtgt ctcgtggtcg ggcgggttcg
    11701 ggtcacccgc ctcgaacagc ggtcgtttcc gtcccgcgaa ctcgatttcc ttgaggatgc
    11761 ttttcaattc gagataatcg gatggagaga attccggcgt tttcaggtga taggtgagtg
    11821 acccggaaag cacggagttg ctgacggatt ggctcatggc aaagaaaacc cccgcgcggt
    11881 cgatatgcac cgcgccgggc agggtgtggg gcgcacccaa ccctgcggcg gcatgcagcg
    11941 cgatccgttc ttccgtgccg caggtgatgc cggtttccag cggataccgg cgtttgtcca
    12001 gtccggtctg tccgatgacg aagttcgcat aacctaatcc ggtgccgagc cagggcatgg
    12061 cgagcaggtc caacccttca ccgcgcacag ggaactccgg aacgcgggtg gtcaaggtca
    12121 ccgcgagcgg ggtttccgtg tcgcgcaaat cctccggggc gatcatacat tcgatcacct
    12181 cggcaccggg aagccggttc ttgagagccg cttcgaaaaa gttgcgccgc tgttccggct
    12241 tctgccggac gaggtaactg cggtaggcgt tgtcattgat gccctcaaac cggatgcgcg
    12301 tcttcagtaa caacgagccg ttacgatcca tcgtgccttc cgtgttaatg cgggtcagat
    12361 tcttctctac ggggtacacg tctgagactc gtagggtttc accctcaggc cgcgccacca
    12421 gatagctgcg gttacagaga taggccggaa aaaggtcgcg ggtgttctcg tccgtggggt
    12481 ccatcaacag gtagccgccg tccgggttat ccacagcggt gacggcgtga ttgaagaacg
    12541 gcatgggcac gtcggggtcc atcttggcgc ccgcgtggat cagcacgggg taggcatcga
    12601 tcccggccat gcgcaacagg gcaaccagta aagcggcctt gtcacggcag accccgtagc
    12661 ggttgtcgaa ggtcagactg acgggatgcg gttcatagcc gggggcggtg tcttcagtcg
    12721 tgatgcccat gtagcggatc tcctgtgaaa cgaatttgaa gatccgccgg atggtctcgt
    12781 cgcgcgaggc ggaacctgcg gtcagggcat cgacccgggt tcgcatggcc gtgtttgtgg
    12841 cggcgagggc gggtgcgcat aactcccaat accagcgcga aacggtccgc caatccggga
    12901 tcgtgctcag caacaaacgc tgcacctggg tgtgcaaggg cggcatatca ggttcgggat
    12961 acatctgcgg cacgttcgtc acggtccaga ggtgaagggt ccggccttca gcttcgcggc
    13021 gctcggtttt catgaccgtg cccggtactg gggcgcgtaa cagcgcatga cggaacggca
    13081 gttccggcgg ggccgagatg gcgtactcca gccggacgat cggcgagtcg tattcaaaca
    13141 ccgcgtagtc ggcccaggtg tcgggcatgc gcaccttgac ggttttgcgg caggtgatca
    13201 gatggcatac gtcgccgatt tcgagacccg gcagtgagag gttgaggatc ttattgttcg
    13261 gatcgtaaat gttggcaccc atctgttcgg attcgaccac cacgcgcgcg tacgatgcga
    13321 tgtcgagtgc aacggttcgg ccgtccggtt tgatgacttc agcgcgatac accagcgcgg
    13381 taccgtaatg cgcattgaac gggatgcgga cggacgaggc gtcacgccga cccttttcag
    13441 tcagaatctt gttgtactcg tcatcccaga ccaccgaggt gccgtcgcgc tcgtaccgtt
    13501 cgagcacgcg gttatccacg agtacgcggt cagcgttcgg aaaacgttcg gcggtgaccg
    13561 aggcggctac ggtcatcacg ctttggcggt caagcgggaa cgcctccggc aggacttcgg
    13621 cacccgatac gtacgggaaa aacggaaaca gggcaaacag aaaaaaagaa cggttcatgg
    13681 gcactccttg tccacccctt tgacgggtgg gacacctttt cgtggcgtcg gtgtcggttt
    13741 cgggatcctc ccgatttgac aggcgtatcc gaaacaggac tagtaaacca aaaacagcgg
    13801 ttccgctcca gtctgctcat gcgtcaggcg gcccccgatc tggttgatcc cgaaagcgat
    13861 cccgaccccg accccgattt ggatgaaacc ctgaaacctt gaatccccga aaccaaagag
    13921 agttgaaggt tcaactgctc tttttaggct aaaaaacagc cgtaccagac ctgttgagtc
    13981 cctcaaggca tacggaggcg gcccccagca gggctgcctc gtatcccaag cttgtcggta
    14041 tgacgcgcag tgactgacat tgc
//
LOCUS       DAOSRB010000059        13933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4466_length_13933_cov_5.955109, whole genome shotgun sequence.
ACCESSION   DAOSRB010000059 DAOSRB010000000
VERSION     DAOSRB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13933
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4466_length_13933_cov_5.955109"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLZ74_04760"
     CDS             complement(<1..199)
                     /locus_tag="PLZ74_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97666.1"
                     /translation="MKNNRFVGWICVTAVFAALVAQAAVTPAPVPAGALKMKRMMKLS
                     NTGSVYNPRICNGEVFAVDISA"
     gene            complement(224..1789)
                     /locus_tag="PLZ74_04765"
     CDS             complement(224..1789)
                     /locus_tag="PLZ74_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR97667.1"
                     /translation="MSCATGVVLAAFCWARADGPFRVLPYLQNPATDAITVMWFSETS
                     TTGQVAFCQTGVWSAPLEVPAVLASALDYHSSETNSPPPLPHPPLPYLHRVRLTGLLA
                     GTAYEYVVTQDEVSITNSFRTAPPRDSAVRFVVYSDSETEPESTGSREKWPEPNGNAD
                     RLYPVDQTRGYQENLQIMAARNPDFIVIAGDLVEAGGEQRDWDEFWRHNAGVLGDLAS
                     TVPIMPALGNHEYYGGTRGGYSQPTSEVSVAKYLTYFELPANGAANPAHNGRYYRLDY
                     GPVTLVVLDLCNGDDTDPAKDTSTYLKTADGCQAPDFNPGSPQYAWLEEQLASAQTNS
                     PFTFVAFHQTPYSVGPHGKADESQSGVPVRVLTPLFLKYGVDAVLAGHDEMYERSVVT
                     GIEQLPGGGTRPVTLSFYDIGIGGDRLREPFSGLVNDKQVFLAHDDAPEMWSGARLVS
                     GGKHYGHLEVNVRVNGQGKWEAVLTPVYAFPLMDSGGTVTGFERRTYADEVTIISASA
                     PLLGQKGAIVTEW"
     gene            2252..2665
                     /locus_tag="PLZ74_04770"
     CDS             2252..2665
                     /locus_tag="PLZ74_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR97668.1"
                     /translation="MARILIVDDEPGLLMFFQRLLNTNGYDVVVAEDGVTALDVIRKT
                     EIDLIISDLRMPRMDGMTLLREVKAIKPDIPVIILTAYASDETAIESKRIGVFEYRSK
                     PFNAEKLLDTIENALGAAKNKEAGPLDSRGGDPVK"
     gene            2732..3046
                     /gene="rplU"
                     /locus_tag="PLZ74_04775"
     CDS             2732..3046
                     /gene="rplU"
                     /locus_tag="PLZ74_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOR97669.1"
                     /translation="MDAYAVLETGGKQYRVAVGNTIEIERLDAEAGQDVVLETVLALS
                     DGQELKVGMPYVPDAKVTLSVIGHKRGKKLINFKKKRRKGYSRRMGHRQELTVATVKA
                     IA"
     gene            3064..3309
                     /gene="rpmA"
                     /locus_tag="PLZ74_04780"
     CDS             3064..3309
                     /gene="rpmA"
                     /locus_tag="PLZ74_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOR97670.1"
                     /translation="MAGAASRNGRDSNAKRLGVKRYDGQVVRAGSILVRQRGTRFHPG
                     KHVGCGRDYTLFALKDGTVKFQKGGAVVSIIPAVAAE"
     gene            3339..4496
                     /gene="obgE"
                     /locus_tag="PLZ74_04785"
     CDS             3339..4496
                     /gene="obgE"
                     /locus_tag="PLZ74_04785"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOR97671.1"
                     /translation="MKTRKIVDRVVVHVRAGKGGDGSASFRREACVPRGGPDGGDGGR
                     GGHVLFRGSRDVDSLIGLSFAPQLFAENGVAGRGQKMYGRRGRDRVVRVPCGTSVFDA
                     ETGELIADVVGHDEEVVIARGGKGGLGNVHFKTATHQVPTEHTPGEPGEEFRLRLELK
                     TIADAGLLGFPNAGKSSLLACLSGARPKIANYPFTTLNPIVGTLMYPDFSQLRVADVP
                     GIIEGAAAGVGLGTSFLKHIARSRVLIFVVDMAGTDNRLPWKDYLALRNEIAQHNAEM
                     LDRPSLVLANKMDVAAAAEYLPRFVRETGVTPIRVSAVTPDDPGVAEVKQSLWDLLRP
                     APRGVWTAVGTAEPETGTPSDGDGEAGIPEVSLRRAPFLDLSKKTPKKKRH"
     gene            4515..6116
                     /locus_tag="PLZ74_04790"
     CDS             4515..6116
                     /locus_tag="PLZ74_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97672.1"
                     /translation="MRIRCAVTIRMILWGAAMTVGIAAAATRPVGADCVPDDLLDDAE
                     AINKALAAAQPGDTVTLPAGVLRLFSPLTPPSGVTLQGAGQERTILAFGGEKPHEFVR
                     MRDVSGVQVCHLTLDGGSNPLATQGVVAMKCDTLCLHHLTIRDFVDTGTFGPHGILLS
                     QTSDSVIRDNTICNIAPEDPWGAGIRVSDRSLRAVIERNVIDRTGRGGIFTNNGASDA
                     VIRENVVTRSGGIGFAIEVHSESVRTLVEDNVVDHGLSIVSSHCAVRRNLVADAGGTW
                     KAYGIEGGGGPYGIVTDNVIHYGQIEGISVSGASHHMLWAHNRFVCCSMWGMQLQGPS
                     ASNHIHSLYFYDNLFTRTQKGHPSVVYKGADGHAIRINNHTTHVTFDGNQITDNGGMA
                     FQILGKNVNHLTFIRNVIANNAGASIRTYLGDAVVWEDNLVSGNKTNVTLTTCGEMGA
                     APVAAFDAPETARAGEPVAFRDTSVDPEGIAQRLWDFGDGLPCLEAQPKHVYRKPGAY
                     RVTLVVWNASKRAALAERIVRVYGE"
     gene            complement(6164..7021)
                     /locus_tag="PLZ74_04795"
     CDS             complement(6164..7021)
                     /locus_tag="PLZ74_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOR97673.1"
                     /translation="MEADLEVYLTERRRLVEEHLSACLPEATPRNKRLIEAMCHAVLS
                     GGKRLRPILCLAAAEASGGSLRDALCPASAVELLHTYTLVHDDLPCMDNDPMRRGQPT
                     VHAKFGEALGVLTGDALQTLAFEILARTPEKQAGTLQRLVTELASAAGQAGVIAGQAE
                     DIRCAAAPTRADIEYVFRHKTADLFRTACRLGAIAANAAAPDLERLTEYGDGLGFAFQ
                     ILDDLLDAHEAKTRAEPELSCLAVMTVAEAREWAENLTQQAVAALAGLPGETAPLEAL
                     ARHLLGRLA"
     gene            complement(7143..9515)
                     /locus_tag="PLZ74_04800"
     CDS             complement(7143..9515)
                     /locus_tag="PLZ74_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97674.1"
                     /translation="MARLTVGLHLTPHPTGGISCTWEDDSGASHTLETCSTETGQVSF
                     PIPIQAKLFRFHFFPSHEPYRINGLSLFHLRLLSAQWLNNMLTPEHPVYTQQYMGKRD
                     CLEIIATEGTSLTYPRFFSTLLHFARRLRTVTPVLLFGLFCLPLGLKLRFCGVHRPFS
                     LPRGLCLPHEEAPLRKGDRSVFIFFVAALLLCLPNPVAPTTPGCDASWSWLLNHFALN
                     PSGIGTSFFFTYGPLGFTITPEAIGNNAFWALAFSLLYILLFSATIHIILTQHETPSA
                     CACPLFLCILLLPPNTEWRWAFLINLMLAACLFFPPEQTRARAVYSAVTGAVILYASF
                     IKFSLAITAGLNVLMVLLLLPPCQRRDLWFFGVPLAGGFLVGLLFARWFLFSSFSSIL
                     TWARLSWEIASGYNTAMVTQVTWLELIIPAILVASFLVLLFPLHSFSLQRFKRMALVS
                     TTLFFAFKYSMTRAGIGTLRAVTYVVPCMTGILMLFADSLWRPNLARLFRFQLAVCVG
                     LSVLTGVFLQLPIIGFNYKNLLDSTRLNQCIAHARTRSDQALLDVRLPPHWIRKIGNN
                     TFSSYPTELTYAPANSLRFIPFPITQGYSAYTQDLDVHGASLFSGPQAPEWLLCAFSK
                     PDQRNGVVDTPAVWNAVRTHYRPEDVNEHGVLLQHRPHTAIPQWRCVRQQTAAVGEWI
                     DLTGKDMTNTCLSIKWSPTLLGKGLALLFRNTQCSFTLIRVDGTTHRWRLVPDTASTP
                     FPIGAIPFTDREFPRIFDNPDSAPFPVSRIKFECEHPWYYSRQITLSFFQ"
     gene            complement(9645..10385)
                     /locus_tag="PLZ74_04805"
     CDS             complement(9645..10385)
                     /locus_tag="PLZ74_04805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR97675.1"
                     /translation="MLISLVLLTWNRYRFLDLCLASLFANLSGAFDYQILLLDNGSTD
                     LTPQVLNRYRNKPGVTLLANAENRGLSAYKTLFNKAKGKIIIEVDDDILDFPKDFDQI
                     MVDYLAAYPDYGFLALNVIQDERTNGAKPDPSHYAEDTRGNKTVEQGPAGGWCAGFRR
                     KDYRAIWLPFNLIPLNMKRCEDGTLTALFRRLRGLKAGVIREAVCLHACGPYYAQQYG
                     QTEREIQKYMAAGLTELADSYIQTQKGV"
     gene            10503..11012
                     /locus_tag="PLZ74_04810"
     CDS             10503..11012
                     /locus_tag="PLZ74_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOR97676.1"
                     /translation="MGNEFKLNLGCGEKVKSGYVNVDACGQPDFLYDLTVFPWPWEDA
                     TVDEVCMEHFLEHVPDYDRTVREIHRILKTGGVLRFTVPHFRNPMAAWHLHCNQFSVY
                     TPELLCEQRAYQWGGRVLFEKISLRIRFVWITPWLGRILSFFANRAPLKWDWLGLPID
                     EIEFVGRKK"
     gene            complement(11060..11953)
                     /locus_tag="PLZ74_04815"
     CDS             complement(11060..11953)
                     /locus_tag="PLZ74_04815"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOR97677.1"
                     /translation="MKKHHILSFIDKTGYGLEIGPSYNPLAPKRLGYNVRTLDYLSQS
                     DLIEKYSRLSVPVAEIEPVDFVWSGEPYSELMGGHSLFDWVIASHVIEHMPDLIGFLK
                     NCTSVLKEDGILAIVVPDKRYCFDHFRPPSSLAQIIDAHQAKLTHPSAGMIAESYLNK
                     AYRNGEGIWNLLTRGPLRFQHTVSETKNAMALREYTDVHAWSFTPHSFRLAIHDLVSL
                     GHLDLKEVSFRITSGFEFLAVFRKGKSPPSSSRLCLAKRAHSELSPSLLLAILERLGV
                     LFYMPLFFVKRWLSPKDRRQG"
     gene            complement(11971..13002)
                     /locus_tag="PLZ74_04820"
     CDS             complement(11971..13002)
                     /locus_tag="PLZ74_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97678.1"
                     /translation="MLALTATLFPLEYVPRLYFLFAVSAVLLVCCFLSFIPWLPPQLK
                     IAFPLTVVLTPHYGEVFFTLTNIHWIVALLFPFLALGVPIRSTSHLTRLSILIVVLAL
                     SDPFILFFYPLFFIRRLAHRGSERGEGVFFLVATFCACLQAGVLLAAPRGAAKTFDTD
                     LWHWLEALLLRPVAGLFLGKEVALLGYTSTPVFILIGLALGLFLCTVLYSIITTTPQN
                     RRILLFFFSVSAIAFTAAFLRSYDRPLVFAPFYGADRYYYIPYAFMIFGVLWFTHTVS
                     GWRKRLGFAACFLILMSSVSVFQLPRRLDSHWTDHIKQLREQGHVRIPINPPGWSVDL
                     YETPTSPDD"
     gene            complement(13275..>13933)
                     /locus_tag="PLZ74_04825"
     CDS             complement(13275..>13933)
                     /locus_tag="PLZ74_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97679.1"
                     /translation="LYHRSTHPQPRVTKRLFGCVNIWNTYDKRGETYFLEQEAFRAAQ
                     AATRFLFVIGWSFRAPDVVLKHKRLIRTIFTPVTRHRDAVARLSGALRAHADHVVGVH
                     IRQTDYKKFSGGRYFYSLDTYKGFMRQMEEQLPGRTRFLLCSDIRLDPTEFRDFDILT
                     GTGHPVEDNYALATCDYLIGPPSTYTAWASFYGGAPKCFLRSTDMRITPECFVIQHSV
                     "
BASE COUNT         3166 a         3604 c         4427 g         2736 t
ORIGIN      
        1 gggcggagat atccacagcg aacacttctc cgttacagat cctcggatta tagacgcttc
       61 ccgtgttgga cagtttcatc atccgtttca tttttaacgc cccggccggc acaggggccg
      121 gcgtgacagc cgcctgtgcg actagggccg cgaacacggc tgtcacacaa atccagccaa
      181 cgaaccgatt gtttttcatg ctgatctcct ctctttccgt ttttcaccat tccgtcacaa
      241 ttgccccctt ctgcccgaga aggggggccg aagcgcttat gatcgtcacc tcgtcggcgt
      301 acgtccgccg ctcgaagccg gtgaccgtgc ccccgctgtc catgagcgga aacgcgtaga
      361 ccggggtcag aaccgcctcc cattttccct gtccgttcac ccggacgttg acctccaggt
      421 gcccgtaatg tttgccgccg ctcacaagcc tcgcgcctga ccacatctca ggcgcgtcgt
      481 cgtgcgcgag aaaaacctgt ttgtcgttaa ccaagccaga aaacggctcc cgcaacctgt
      541 cgccgccgat gccgatatca tagaagctga gcgtgacggg gcgcgtgccg ccgccgggca
      601 actgctctat ccctgtcacg acggagcgct cgtacatctc atcatgtccg gccagtacgg
      661 catccacgcc gtatttcagg aaaagcggcg tgaggacacg cacgggcaca cccgactgtg
      721 attcgtcagc ctttccgtgc ggcccgacag aatacggggt ctggtgaaag gccacaaagg
      781 tgaacggact gttggtctgg gccgaggcga gctgctcctc cagccaagcg tactgcggcg
      841 agccggggtt gaagtccggc gcctgacagc cgtcggccgt cttcaggtac gtgctggtgt
      901 ctttggcggg gtccgtgtca tcgccattac acaggtccag caccaccagc gtcaccggtc
      961 cgtagtccaa gcggtaatac cgtccgttat gggcggggtt tgccgcgccg ttggcgggca
     1021 gctcgaaata ggtcaggtat ttggcgacgc tcacctcaga ggtcggctgg ctgtaaccgc
     1081 cgcgcgtacc gccgtagtat tcgtggttgc ccagcgcggg catgatgggc acggtgctgg
     1141 ccagatcacc cagaacaccg gcgttgtgac gccagaactc gtcccagtcc cgttgctcgc
     1201 cgcccgcctc aacaagatcg ccggcgatca cgataaagtc cgggttgcgg gcagccataa
     1261 tctggaggtt ttcctgatac ccgcgtgtct ggtcgactgg atacaggcgg tcggcgttac
     1321 cgttcggctc gggccacttc tctcgggagc cggtcgattc cggttctgtc tcggagtcgg
     1381 aatagacaac gaagcgcacg gcgctgtctc gcggcggtgc cgtgcggaac gagttggtga
     1441 tcgacacctc gtcctgcgtg accacgtact cgtaggcggt tccggccagc agccctgtca
     1501 gcctgacccg gtgtaaatag gggaggggcg gatgggggag gggcgggggc gaattcgtct
     1561 cgctggagtg gtagtccagt gcgctggcca gcactgcggg aacttcaagc ggcgccgacc
     1621 ataccccggt ctggcagaag gcaacctgac cggtggtgga ggtctccgag aaccacatca
     1681 ccgtgatcgc gtcagtcgcc gggttctgca ggtacggcaa gacgcggaac ggaccgtcgg
     1741 cccgagccca gcaaaaagca gccagtacca cgccggtcgc gcatgacacg cacgcccccg
     1801 cgggacacgg aattttcaga gcatccgttc ttgtgcggtc atcgcgtgat gttccagtaa
     1861 ggtccattgg gcaaacgctc acgatatttc gcacttttgt tgtgaagcca caagtgcatc
     1921 cagccacgga taacaagctt cacacggata atagtttcgc acttccagca tatcgtgtca
     1981 atcggcttta ttcacggagc cgaacggacc atgaatcttc gggcgcacgg taatcagttc
     2041 gtgacaggga gtgtgcgaag tcgcgaacct gtcacgggcg cacggctttt cgttgttgac
     2101 gggaaaacgg atgcccctta cagtgatgtg taacggtaga tgacaggttt cagtcccaaa
     2161 ccttcgtgca gtggagagcg cgatgccctg agtgagggac gggaacgtgt gtgatgccta
     2221 caggcgccta tcaggagaaa gagaattggt tatggccaga atcctcatcg ttgacgacga
     2281 gccggggctg ctgatgttct ttcagcggct tctcaacaca aacggatatg atgtggtcgt
     2341 ggcggaagac ggggtgacgg ctctggacgt gatccgcaaa acggagatcg atctgatcat
     2401 ttccgacctc cgcatgccgc gcatggacgg catgaccctt ttgcgcgagg ttaaggcgat
     2461 caagccggat atccccgtga tcatcctgac ggcctacgcg tcggacgaga ccgcgatcga
     2521 gtccaagcgg atcggggtct tcgagtaccg ttccaaaccc ttcaatgcgg agaaacttct
     2581 cgacaccatc gagaacgcgc tcggtgccgc caaaaataaa gaggctggcc cgcttgacag
     2641 tcgcgggggc gatccggtaa agtaaaatcc ttttgttccg atttggaaag gtgcggaacc
     2701 gctcggttcc ccagtgatga agggtcaaaa catggatgcg tatgcggttt tagagaccgg
     2761 cggcaagcag tatcgtgtgg ccgtgggcaa cacgatcgag attgagcgtc tggatgccga
     2821 ggccggtcag gacgtggtgt tggagacggt gttggcgctc tcggacgggc aggaactgaa
     2881 ggtcgggatg ccgtatgtcc cggacgcgaa ggtgacgctc tccgtgatcg gccacaaacg
     2941 cggcaagaaa ctgattaact ttaagaaaaa acggcgcaag gggtactcgc gccggatggg
     3001 gcaccgccag gaactgacgg tggccacagt caaggcgatc gcctaaccgg aggagtgtga
     3061 cacatggcag gcgcagcatc aagaaacggg cgggacagca atgcgaagcg gttgggcgtg
     3121 aagcggtatg acgggcaggt cgtgcgcgcg ggatcgattc tggtccggca gcgcggaacc
     3181 cggttccacc ccggaaagca tgtcggatgt ggacgcgatt acaccctgtt cgctttaaag
     3241 gacggcaccg tcaaattcca aaaaggcggc gcggtggtca gcatcatccc cgcagttgcg
     3301 gcggagtgat ctgatccggg tctgcggcgg actgcggcat gaagacaaga aagattgtgg
     3361 atcgtgtcgt ggtgcatgtc cgcgccggta agggagggga cggaagcgcg agtttcagac
     3421 gggaggcgtg tgttcccaga gggggccccg acggcggcga tggcggccgt gggggccatg
     3481 tgcttttccg gggcagccgt gatgtcgatt cgctgatcgg tctctccttc gcgccgcaac
     3541 tgttcgccga aaacggggtg gcgggacgcg gccagaagat gtacggacgg cgggggcgcg
     3601 accgggtggt ccgggtgccg tgcggcacaa gcgtgtttga tgccgagacc ggggaactga
     3661 tcgcggatgt cgtgggccac gacgaagagg ttgtgatcgc gcggggcggc aaaggcggcc
     3721 tcggcaacgt gcatttcaaa accgcgacgc atcaggtgcc aacggaacac acgcccgggg
     3781 agccgggtga ggagttccgg ctccggttgg aactgaaaac cattgcggat gcgggcttgc
     3841 tcgggttccc gaacgccgga aagtcctcgt tgctcgcctg tctgagcggc gcgcgtccga
     3901 agattgccaa ttacccgttc accacgctca atccgatcgt gggaacgctg atgtatccgg
     3961 atttctccca gcttcgcgtg gccgatgtgc cggggattat cgaaggggcc gctgcagggg
     4021 tcggtctcgg caccagtttt ctgaaacaca tcgcgcggtc gcgtgtgctg attttcgtgg
     4081 tcgatatggc ggggacggac aatcgtctgc cgtggaagga ctatctggcg ctgcggaacg
     4141 agatcgcgca gcacaacgcc gaaatgctcg accgcccctc cctcgtgctg gccaacaaga
     4201 tggatgtggc ggccgctgcg gaatacttgc cgcgctttgt gcgcgagacc ggcgttacgc
     4261 cgatccgggt ttctgcggtg acgccggacg atccgggcgt ggcggaggtg aagcaatccc
     4321 tttgggattt gctgcgccct gcgccccggg gcgtgtggac tgcggtcggc actgcggagc
     4381 cggaaaccgg caccccttcg gacggggatg gggaggcggg tatccccgag gtctcgctcc
     4441 ggcgggcgcc gtttctggat ctttccaaaa agacgccgaa gaaaaaacgg cactgagtgt
     4501 aaaaaggagg agagatgcgg attcgttgtg cggtaacgat acggatgatc ctgtggggtg
     4561 cggccatgac ggtcggcatc gcggccgccg caacccgtcc ggtgggtgcc gactgcgtgc
     4621 cggatgattt gctggatgac gccgaggcca tcaacaaggc gttggccgcg gcgcaaccgg
     4681 gggacacggt aacgctcccc gcaggcgtgt tgcgcctgtt cagcccgctc accccgccct
     4741 ccggcgtcac actgcaagga gcggggcagg agcggaccat tctggctttc gggggggaaa
     4801 aaccgcatga atttgtgcgg atgcgtgatg tctccggggt gcaggtgtgc cacctgacac
     4861 tcgatggcgg cagcaacccg ttggcgaccc agggggtggt tgcgatgaag tgcgacaccc
     4921 tttgcctcca tcacctcacg atccgggatt tcgtggacac ggggacgttc gggccgcacg
     4981 ggattctctt atcgcaaacc tctgattcgg tgatccgcga caacacgatc tgcaacatcg
     5041 cgccggaaga cccttggggc gcggggatcc gcgtgtcgga ccgatcgctg cgggccgtga
     5101 tcgagcggaa cgtgatcgac cggaccggtc gcggcggcat tttcacgaac aacggggctt
     5161 ccgacgcggt gatccgcgag aatgtggtca cgcgctcggg tggtatcgga ttcgcgatcg
     5221 aagtgcacag cgaaagcgtg cggaccttgg ttgaggacaa cgtggtcgat cacgggttga
     5281 gcatcgtcag ttcgcactgt gccgtgcgcc gtaatctcgt ggcggatgcg ggcggaacgt
     5341 ggaaggctta cggcatcgag ggcggtgggg gaccctacgg gatcgtcaca gacaacgtca
     5401 tccattacgg ccagatcgag ggaatttccg tctccggcgc atcccaccac atgctttggg
     5461 cacataaccg gttcgtctgc tgctcgatgt ggggcatgca gcttcagggg ccttcggcga
     5521 gcaaccatat ccacagcctc tatttctatg acaatctttt cacccgcacc cagaaaggcc
     5581 atccttcggt ggtctacaag ggggcggacg gacacgccat ccggatcaac aaccacacca
     5641 cccacgtcac gttcgacggc aaccagatca cggataacgg cggaatggct ttccagattc
     5701 tggggaagaa tgtcaaccat ctcaccttca tccgcaatgt gatcgccaac aacgcggggg
     5761 cgtccatccg gacgtatctg ggtgacgcgg tggtgtggga ggacaatctg gtttcgggca
     5821 acaagaccaa cgtgacgctc acgacctgcg gggagatggg tgccgcaccg gttgcggcgt
     5881 ttgacgcgcc ggaaacggcg cgcgcgggcg agccggtcgc tttccgcgac acatcggtgg
     5941 atccagaggg aatcgcgcaa cggctctggg atttcgggga cgggctgccc tgtcttgagg
     6001 cccagccgaa acacgtgtac cggaagcccg gcgcgtatcg cgtcaccctt gtggtctgga
     6061 acgccagcaa gcgtgcggca ctcgccgaac gcatcgtgcg cgtgtacggc gaatagaccc
     6121 gtcttcgcgt tagggtggcg gcgcgtcggc cgggccgcgg ttctcaggcg aggcggccga
     6181 gcaggtgacg cgcgagggct tcgagcggcg cggtctcgcc gggcaggcct gccagtgccg
     6241 cgacagcctg ttgcgtgaga ttttccgccc actcccgcgc ctccgcgacc gtcatgaccg
     6301 cgagacagga gagttccggt tcggcccgcg tcttggcctc gtgggcatcg agcaggtcgt
     6361 cgaggatctg gaaggcgaat ccgaggccgt ctccatattc ggtcaggcgt tcaaggtcgg
     6421 gtgccgcggc gttggcggcg atcgcgccga gacggcaagc cgtgcggaag aggtcggcgg
     6481 ttttatggcg gaagacatac tcgatgtctg cgcgcgtggg cgcggccgcg caacggatgt
     6541 cttcggcctg gcccgcgatc acgccggcct gtcctgcggc ggaggcgagt tcggtgacga
     6601 gccgctgcag ggtgccggcc tgtttttccg gtgtgcgcgc gagaatctcg aaggccaggg
     6661 tttgcagggc gtcgccggtc agcaccccga gcgcctcgcc gaatttcgcg tgcacggtgg
     6721 gctgtccgcg ccgcatcggg tcgttgtcca tacaaggcag gtcatcatgc acaagcgtgt
     6781 acgtgtggag caactccacg gcggaggcgg ggcagagggc gtcacgcaag ctgccgcccg
     6841 aggcttcggc cgcggcgagg cagaggatcg ggcgcagccg cttgccgccg gagagcaccg
     6901 catggcacat ggcttcaatc agtcgtttgt tgcgtggcgt ggcttcgggc aggcaggccg
     6961 agaggtgttc ttcgacaagc ctgcggcgtt cggtgagata gacttccaga tcggcttcca
     7021 tgtggggcag tatacgccaa ccgtccgaac cgggcaagcc gcaggcaggc agatttttgt
     7081 tgctagccta tcttaaaagg atcgtgcgtt tgtatggagt aaacagactt tatcggaaaa
     7141 ggttactgaa aaaaggagag cgttatttgc cgcgaataat accaagggtg ctcgcattcg
     7201 aatttgatgc gtgacacggg gaaaggcgct gaatccgggt tgtcgaaaat ccgggggaat
     7261 tcgcgatccg tgaaggggat ggccccgatc ggaaagggag tgcttgcggt gtcgggcacc
     7321 agacgccacc gatgtgttgt accgtccacc cggattagag taaaggagca ttgtgtattc
     7381 cggaagagaa gggctaaccc ttttcccagc aaggttggcg accacttgat tgacagacag
     7441 gtgttggtca tgtcctttcc ggtcagatcg atccactctc ctactgcggc agtttgttgt
     7501 cgcacacatc tccattgtgg gatcgcggtg tggggccggt gttgaagaag aacaccgtgt
     7561 tcgttcacgt cttcgggacg gtagtgggtt cgaacagcgt tccagaccgc cggggtgtca
     7621 acgactccgt tccgctggtc aggcttgctg aatgcgcaaa gcaaccattc gggtgcttgg
     7681 gggccggaaa acaacgaggc gccgtggaca tcaagatcct gcgtgtatgc ggaataccct
     7741 tgcgtgatcg ggaaaggaat gaaccgcagt gaatttgcgg gagcgtaggt caattctgtc
     7801 ggatacgaac tgaacgtgtt gttgccgatt ttccggatcc agtgaggcgg aagtcgcaca
     7861 tcgagcaatg cttggtccga ccgggttcgc gcatgggcga tacattgatt cagacgggtt
     7921 gaatcaagga ggtttttgta attgaagccg atgatcggca actgtaggaa aacaccggtt
     7981 aagaccgaaa gtcctacgca tacggcaagt tggaaacgga agagtcgggc aaggttcggg
     8041 cgccaaagag aatccgcaaa cagcatgagg atgccagtca tacagggaac cacataggtt
     8101 acggcacgca gggtaccgat gccggcgcgg gtcatactgt atttgaaagc gaaaaacagg
     8161 gtcgttgaaa ccagtgccat tcgtttgaaa cgctgaaggg agaatgaatg aagcggaaag
     8221 agaaggacaa gaaaactggc cacaagaatt gcagggatga tgagttcaag ccatgtgact
     8281 tgtgtgacca tagccgtgtt atacccggaa gcgatttccc aggagagacg tgcccaagtc
     8341 aagatggatg aaaaagacga gaataaaaac caacgtgcga agaggaggcc caccaagaat
     8401 ccgcctgcca acggaacccc gaaaaaccat aagtcgcggc gctgacatgg ggggagaaga
     8461 agaaggacca tgagcacatt gagccccgcg gtgatggcta aggagaattt gatgaacgac
     8521 gcatagagga tcaccgctcc ggttacggcg gaataaactg cgcgtgcgcg tgtctgttcc
     8581 ggcgggaaaa agagacaggc cgccagcatc aggtttatca ggaaggccca gcgccattca
     8641 gtgttgggcg gcaggagaag gatgcagagg aatagagggc aggcacaggc ggagggcgtt
     8701 tcgtgttgcg tcagaatgat atggatcgtt gcgctgaaca ggaggatata cagcagagag
     8761 aaggccagag cccaaaaggc attgtttccg atcgcttcag gtgtgatcgt gaatcccagt
     8821 ggaccgtaag tgaagaaaaa agaggtcccg atccctgagg ggttcagggc gaaatggtta
     8881 agcaaccacg accaagatgc atcacatccc ggggtggttg gtgcgacagg gttcggcagg
     8941 cacagaagga gggccgcgac gaaaaaaata aaaaccgaac gatctccttt tctcaacggc
     9001 gcctcctcat gtgggaggca aagacctcgc gggagagaaa aggggcggtg tacgccgcaa
     9061 aatcggagtt ttaggccgag cggtagacaa aacaggccga atagcaaaac aggggtgact
     9121 gtgcggagtc ttctggcgaa gtggagaagg gtggaaaaga accggggata ggttaacgat
     9181 gttccctcgg tggcgatgat ttcgagacag tcccgtttgc ccatatattg ttgagtatag
     9241 acgggatgtt ccggggtgag catattatta agccattgcg cgctcagcaa ccgaagatga
     9301 aagagggata gcccgtttat tcgatacggc tcatggctag ggaaaaaatg aaaccggaag
     9361 agtttggcct ggatggggat cgggaacgag acctgtccgg tttcggtcga acaggtctcc
     9421 agagtatggg atgctcccga atcgtcttcc catgtacagg aaatgccgcc ggtcggatgg
     9481 ggagtcaggt gcaaaccaac ggtcagccgc gccatccgga cgtaaagacc tgcacacagg
     9541 acacccgaac aaaccgttaa catcagaaga gcgcgtacgg atactgatcg gagggttgtt
     9601 gggatcgaca taaggataaa cctcacaaga acagagagaa gaaattaaac gcctttttgt
     9661 gtctgaatat acgaatcggc aagttccgtt aatcctgcgg ccatgtattt ttgaatctcc
     9721 cgttccgtct gcccatattg ctgcgcgtaa taggggccgc aggcatgaag acagaccgcc
     9781 tcgcggatta cacccgcttt taaaccccgc agcctacgaa acagggcggt taaggtgccg
     9841 tcctcacatc gcttcatgtt taacgggatc aaattgaaag gtagccaaat cgcacggtag
     9901 tctttgcgac ggaaccccgc gcaccaaccg ccggcaggcc cctgttcaac cgttttgttg
     9961 ccccgggtgt cttccgcata atgagaagga tcaggtttcg cgccattcgt ccgttcgtcc
    10021 tgaatgacat tgagggccaa aaacccataa tccgggtaag ccgccaaata gtccaccatg
    10081 atttggtcaa aatcttttgg aaaatcgagg atatcgtcat cgacttcgat gatgattttc
    10141 cccttggctt tattgaagag cgtcttatat gcgctcagtc cccgattttc cgcattggcg
    10201 agaagggtca ctccgggttt atttcgataa cggttcaaaa cttgaggcgt aagatccgtc
    10261 gagccattat ccaaaagaag aatttggtag tcgaatgcac cggaaaggtt tgcgaacagc
    10321 gacgcaaggc ataaatcgag aaaccggtaa cggttccacg tcaacaggac aagcgaaatc
    10381 aacatacgac tcccgggcaa aagtccatcc gtgtcaacga aacatctttt ccagacttca
    10441 aaacccagat atactagcac acgttactga ataaataaac cggcaaaggg aaggcgttca
    10501 gaatgggaaa cgaattcaaa ctcaatctgg gatgcggcga gaaggtgaag tctggatatg
    10561 tgaatgtgga tgcttgtggc cagcctgatt ttttgtatga ccttacagtt ttcccctggc
    10621 catgggagga cgccacggtg gatgaggttt gtatggagca ttttctggag catgtgccgg
    10681 attatgaccg gacggttcgg gagattcacc ggatcttgaa gacgggaggg gtactgcgat
    10741 ttacggtgcc acacttccgg aatccaatgg cagcgtggca tttacactgt aatcaattca
    10801 gcgtatacac accggaatta ctgtgcgagc aacgggcata tcagtggggc ggaagggttc
    10861 tttttgaaaa aatctccctc cggatccgtt ttgtctggat taccccttgg ctcggccgaa
    10921 tcttgtcatt tttcgccaac cgggctccgc tcaaatggga ttggttgggg ctgccgattg
    10981 atgaaatcga attcgttggg cgtaaaaaat gatcggcggg ttatgttttg tttgcgtatt
    11041 tcagaaaacc gtgaattttt tacccttggc gtcggtcttt tggggacagc catcttttga
    11101 cgaagaaaag aggcatataa aaaagtacac ccagccgttc aagtatagcg agaagaagag
    11161 aaggcgagag ttcggaatgc gcccgcttcg ccaagcacaa gcgagaactt gacgggggcg
    11221 atttgccttt ccggaagaca gcgaggaatt caaagccgga cgtgatacga aacgaaacct
    11281 ccttcaaatc aaggtgtccg agagaaacga ggtcatgtat ggctagacgg aacgagtggg
    11341 gtgtgaaact ccatgcgtgt acatcagtat actctcggag cgccatcgcg ttttttgttt
    11401 cgctgaccgt gtgttggaaa cgcaacggtc cgcgagtgag caggttccaa atgccctctc
    11461 catttcgata cgccttattc aaatagctct ccgcgatcat ccccgctgag gggtgcgtca
    11521 gcttcgcttg gtgtgcgtcg atgatttgcg caagcgaaga gggggggcgg aagtgatcga
    11581 agcaataacg cttgtccgga acaacaatcg cgagaattcc atcttccttt aagacggagg
    11641 tgcagttctt cagaaatccg atcaggtccg gcatgtgctc gatgacatgt gaggcgatga
    11701 cccagtcaaa aagtgaatga cctcccatta attccgagta gggttctccg gaccagacga
    11761 aatcaacggg ttcaatctcc gcgaccggga cagaaagacg cgaatatttt tcgattagat
    11821 cggactgtga taaataatca agcgtacgga cattataacc gagtctcttg ggggcaaggg
    11881 gattgtaact gggaccgatt tcaaggccat atcctgtctt gtcgatgaag gagaggatgt
    11941 gatgtttttt cataagaaaa cggcggacgg ttaatcatcg ggtgaagtgg gtgtttcata
    12001 caaatccacg ctccatccgg gcggattaat ggggatacgg acatggccct gttcgcgcaa
    12061 ctgtttgatg tgatctgtcc aatgtgagtc gagtctgcgg ggaagttgga aaacggaaac
    12121 agaactcatt aaaatcagaa aacacgccgc gaaccccaac cgttttctcc atccggatac
    12181 tgtatgggtg aaccagagta ccccaaaaat catgaaagca taaggaatat aataataacg
    12241 gtctgcccca taaaagggag cgaagaccag aggacgatca tacgatctca aaaatgctgc
    12301 ggtgaatgcg atggccgata ctgaaaagaa aaacaaaagg atccggcggt tttgtggtgt
    12361 ggtcgttatg atggagtata gtacggtgca gaggaagagt cccagcgcga ggccgatgag
    12421 gataaacacc ggggtggagg tgtatcccag tagcgcgacc tccttcccaa gaaaaagccc
    12481 cgcaaccgga cgaagcaaaa gggcttcgag ccaatgccag agatcggtgt cgaatgtttt
    12541 cgcggctccg cgcggggcag caaggagaac accggcctgc aggcaagcgc agaaggtggc
    12601 gacaagaaag aaaactcctt ctccgcgttc ggagcctcgg tgtgctagcc tgcggataaa
    12661 aaaaaggggg tagaaaaaaa gaataaaagg atcacttaga gcaagcacaa cgatcaggat
    12721 ggacaaccgt gtcaaatgag acgttgaacg gatgggtact ccaagggcaa gaaaggggaa
    12781 caacagtgcg acgatccaat ggatgtttgt tagcgtgaag aaaacctccc cgtaatgcgg
    12841 tgtgagcacc accgtgaggg ggaacgcgat tttcaattgc ggcgggagcc atggaatgaa
    12901 ggagagaaaa caacaaacca atagcacggc ggagaccgca aaaaggaaat ataagcgcgg
    12961 cacatactca agggggaaaa gggttgctgt cagagccaac atgcgcggca acacatgtaa
    13021 ataccccgcg tgcggtatgg caatcgaagc gatcccgtgt gttcgcgctt gggcgaggaa
    13081 aatcggtccg tcctctcccc aaagttgagg cgagacgaac tcatggggtg cccggaaaaa
    13141 aaggagggct gtgattccga gcaggaggat gacacgcgac gaccatgtga tatccgcctg
    13201 gatgggcaga ttgagaggta gccaaggact aagacgagac gcattactgg atgtttgcat
    13261 agcgaaaaga gaggttagac cgaatgttga atgacaaagc attccggtgt gatacgcata
    13321 tccgttgatc ggaggaaaca tttcggtgcc ccgccgtaga acgaggccca ggcggtgtag
    13381 gtgctggggg ggccgattag ataatcgcag gtcgcgaggg cgtagttgtc ctcaaccggg
    13441 tgcccggtgc cggtcaggat gtcgaaatcc cggaactcgg tcgggtccag ccggatgtcg
    13501 gaacagagga ggaagcgggt tcggcccggc agttgttcct ccatctgccg catgaaccct
    13561 ttgtaggtgt ccagagagta gaagtatctg ccgccggaga actttttgta gtccgtctgg
    13621 cggatatgca cacccacgac atgatcggca tgggctcgca acgctccgga caggcgcgcg
    13681 accgcgtcgc ggtggcgggt gacgggggtg aatattgtcc ggatcaggcg cttgtgcttc
    13741 agcacgacat ccggagcccg gaaagaccac ccgataacga acaagaagcg cgttgcggct
    13801 tgggcggcgc ggaacgcttc ctgttcaaga aaataggttt cgccccgttt atcgtaggtg
    13861 ttccaaatgt tgacgcagcc gaacaggcgt ttcgtgacgc ggggctgggg gtgtgtcgaa
    13921 cggtggtaga gga
//
LOCUS       DAOSRB010000060        13891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4487_length_13891_cov_7.670642, whole genome shotgun sequence.
ACCESSION   DAOSRB010000060 DAOSRB010000000
VERSION     DAOSRB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13891
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4487_length_13891_cov_7.670642"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..1571)
                     /locus_tag="PLZ74_04830"
     CDS             complement(285..1571)
                     /locus_tag="PLZ74_04830"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOR97680.1"
                     /translation="MESSSYNPFQVAQSQFDKVAAVLELDGGVRDLLRQPMREYQFSI
                     PVRMDDGTVRVFKGFRVQHNDARGPAKGGIRFHPMETVDTVRALSMWMTWKCSVVDIP
                     LGGGKGGVVCDPHHLSLREQEQICRGWIRQVVRNLGPVSDVPAPDVMTNAQHMLWMLD
                     EFETIRGGRYPGFITGKPVGMGGSLGRTEATGFGVVFTLREALRELGIELRKTTASVQ
                     GFGNVAQHAVQLYTELGGTVVAISCWDQTDKTSYTFRKADGVCLKELRGISDKFGGID
                     KNRARELGYEMLPGGAWLEQEVDILIPAALENQVNRDTVQKISKSVRVIAEGANGPTT
                     PEADEVLQQRGIFVLPDFLTNAGGVTCSYFEQVQCNTNYFWEKDEVLAKLDTKMTSAF
                     AAVSDLARRKKLYLRDAAYMIAINRVAEAVRLRGWA"
     gene            complement(1686..3371)
                     /locus_tag="PLZ74_04835"
     CDS             complement(1686..3371)
                     /locus_tag="PLZ74_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97681.1"
                     /translation="MARGILLAALWAAAGGCRALREPESGPLPIRCYGEEAARLRGLP
                     LTREIPVTSEAHDVWMAVLEAELDKPENRRFLEETERLARHFRVLPADVSLRALYRAL
                     MREQVAAYYDPEKKRIAVVEVPGSGYPEGAANRALVDRFVYTHEYCHAVEDVHFDLER
                     LTRAALSDLDANLALTSLVEGNAVLVGADNLCAAWPVNTATPLGAFLVSLVSRLELTD
                     TVGAGLDAYPAFLAGALVRPYLDGAVFSNRLRREAGWRALDRVYRERLPVTTAEILYP
                     ERRYLRPFQPAEISLASDVMGQGPDVMQPVSTNRLGVLGTALWLGGERLASVRDFGFL
                     QGWLGDRVFLAEAPDGTTCTIWLSYWERPGHARAFRKQVLRRLESDAFTGVLHAVRRE
                     GRLVAAVWMSGPGAEEAVCGEKASRALASRVEAETPSWFASWWEDLPSPVSVGPSGCG
                     VLGGWAAELLGGTGFFHVRLACGGLLRVEHTPDRHYWGTAFGLLRHVGDRRSDFTFWQ
                     IPLVASWHAQGQGERRRFRWSVGAGLVATGEPDRIRVLRIPVWRSATAGKNIP"
     gene            complement(3445..4638)
                     /locus_tag="PLZ74_04840"
     CDS             complement(3445..4638)
                     /locus_tag="PLZ74_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOR97682.1"
                     /translation="MRSTLTVLSLTVVALLGLGIVVLATASAVRAQGLYADPHFFVRR
                     QLIWLGLAAVTVFLINHFDYHWWRNTPLLTVGFYLLVVLALLLVFVPGIGGKVKGSYR
                     WLNLGPFRMQPSEFAKILTVIGISVWMDRIGWRVRQFTQGALLPGCGLALLVVLLVME
                     PDFGSTLVVCVVGGALMYVAGTRFVYLLLFGGLGLAGVGAFLVTNANRMRRIAAWLPE
                     QFRLIIERWLPPGHTEEGASAAKYQLEQAILAFKNGGPSGVGFNQSIQKYNYLPEAHT
                     DFIFAIGGEELGLAFSLFVVAAYTVILFCGIRIALRAADRLGRMLAFGMTLLLVFQAA
                     FNIGVVTGCLPTKGLALPFMSYGGTNLITALFAVGTLLNVGRHVDVFDERMHTQVVRN
                     AAQEV"
     gene            complement(4658..5650)
                     /locus_tag="PLZ74_04845"
     CDS             complement(4658..5650)
                     /locus_tag="PLZ74_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOR97683.1"
                     /translation="MNRQVMRVVAGVNLIVGCFLMQGCGMFNRKSDTTSTTLPDGELP
                     PVEVQPLDTTPPPVQPVETSLVELPSVQTSTPYTIQKGDTLSAIAYKYNVRWQDIVAI
                     NPGIVPNRLRIGQVIQLPGQVDLSRARSVPVATPGVRAPKTVAPKAAAAVPGPTITYV
                     VKPGDSLSVIAHKHGIKTAALREANGLKSDKIIVGQKLKVPGAVKTPSAAPVTKKSAA
                     SVQAPKTVAPKPSAAVKTEPAPAVTTTPPPAAEKTTAPAAVESAPVKIETAPPPAAPA
                     AESSTQTYTVKEGEDLYAVAIRWGVSPSDLKALNNLTSSELKAGTVLKIPNAGQ"
     gene            complement(5694..7037)
                     /gene="murD"
                     /locus_tag="PLZ74_04850"
     CDS             complement(5694..7037)
                     /gene="murD"
                     /locus_tag="PLZ74_04850"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOR97684.1"
                     /translation="MSKYDGVQALVLGAGISGEAAARLLLARGGRVRVIDEGKADASA
                     AVAQRITAAGGQVTFGAAGLPSEPFDVCVVSPAFALNHPWITACRARGLPMVSELELG
                     SEYWSGRVIAITGSKGKSSLVKWCSDTLTGAGIPASPAGNYGIPLCQLALDRPDLLWA
                     VTEVSSFQMEHTRTFHPEIAILLNLQADHQDRHVDMTEYKSAKMRLFASMCPGDVVLL
                     PAGFEDDGCVPPGVTLWRFGTDVGCDWRYADHAVTGQAEGTRVRVETGGSWFDNEVFG
                     LSLSAGVAALHAAGLDAEQIAAGVRAFEPLPHRMQLVWESRHGVRYVDDSKATSLAAT
                     AAALRMARKPVRLIAGGLLKEPDLEGIKELLTKTTKKGYLIGHCSRQMFQAWSDVIPC
                     EECGTLDQAVAAAAREARAGDTVLLSPGTASFDQFSSYRERGERFTAQVRAVADL"
     gene            complement(7024..8418)
                     /gene="murF"
                     /locus_tag="PLZ74_04855"
     CDS             complement(7024..8418)
                     /gene="murF"
                     /locus_tag="PLZ74_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOR97685.1"
                     /translation="MSNKQPQRLDAAAFAAWTGGDAQALPSTFCGVTQDSRRITPGCL
                     YVALRGERFDGHDFVAQALRDGAAAALVETAWSAPSGSSGWPLIRVKDTRQALADAAR
                     AWRRLNRAFILGITGSSGKTTTKEMAAALFAAGGRVCATRGNLNNEVGLPLSLLAMPP
                     ETEYGIFELGSNHPGEIGRLSDTLQPCAAVITSIGCAHIEHFGTQEAIVREKGALLRA
                     LPHGGFAVLNREITGFAQLAEQSGAPVVTVTLTGGGADFSAEATDLQTGALRITEQAT
                     GEETRLCSGLPGAHNASNLLLAFAAARRAGLPACALAQALSGLTLPKMRWEVMVRDGV
                     TWVNDAYNANPQSMAAALKTFAELPCRGRRIVVLGDMLELGEHTEALHREVGRCVAQT
                     APDRLLTVGHDAKLYLAEEAVRAGFPQTKVVCADDARAAGIVLCEWIRSGDSVLLKAS
                     RGMRLEGIVGHEQV"
     gene            complement(8445..9935)
                     /locus_tag="PLZ74_04860"
     CDS             complement(8445..9935)
                     /locus_tag="PLZ74_04860"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOR97686.1"
                     /translation="MKISEIPELAPLLVSEAPTVAFTGIQCDSRRIRPGDLFAAITGT
                     RDAGGRYAGAALAKGAVAILSETPLPEYHRQVILVRDVRLALACIASALKAWPSRNMR
                     VYGITGTNGKTTTAWLLREMLVAGGAVPGLLTTVQVEYAGRAIPATRTTPDACELQAI
                     LAAMRTSGCDTAVMEVSSHALDQQRVAWTRFAGAAFTNLSRDHLDYHGTMEAYFVAKC
                     KLFDQLAAETPGALAVCVADAGYGDRMTAYLATLPLRRLTCGFTQAADLRAEALAVTP
                     DASTFTLAAPDGRRLALRVCLAGRYNVANMLCAAGLALEAGVPLEKVGETLEAARPCW
                     GRLERVPVDLPATVFVDYAHTDDALTNVLETLREVTRGRLRVVFGCGGERDRAKRPLM
                     GRACAALADDLVITSDNPRSEDPRAIIEEILQGIPEGTSYEVEADRRLAIRRALSQAR
                     EGDVVLIAGKGHESFQELAGRTLPFDDRQVVAEEARILSEARQQDR"
     gene            complement(9971..11689)
                     /locus_tag="PLZ74_04865"
     CDS             complement(9971..11689)
                     /locus_tag="PLZ74_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOR97687.1"
                     /translation="MHDRGIQMRILLVTAALFFALLGLASRLAYLHLANHSKLTREYR
                     RTLLGLRGRIFDCNGDRFPMAVSLPARQFFLDPKSVKPQHDKKQIAETVAACLGLEAD
                     EVYAQFARTDSRYIKLGVSLNDKVFDLLADPETISGIGSEDLVCRSYPQGRRMSHVLG
                     FVNAMGVGGAGIEQQYNRYLTGTPGLIEGEKDAGRREIWSRRTVHVPAIAGSDLYLTL
                     DHNIQYEVELALREVVEKFEAAGGWVIVQRVRTGEILAMASFPDFAPEQYTEESVDVW
                     RNSALAIVYEPGSIMKAITVAAALNERIVTPETVIDAGEGCWMYAGKPLRDHVHGRVT
                     VSTALKKSSNIACAKIGLMLGNKRLETYLRAFNFGEKLGIELPGEERGILARARDWDK
                     LKPTRIPIGQGVAVTGLQMISAYSTLANDGVMMKPYVVRRIVSPSGETIFEAKPEVLG
                     RPVRPEVARAVREMLRGVTEEGGTGKRASVPGYSIGGKTGTAQKAVPGGYSSTDYYAS
                     FVGFVPAGDPVFSVLVSVERPRPQHTGGFVSAPAFAKIAAATARYLEVPPDQPAEETE
                     QVANHP"
     gene            complement(11721..12116)
                     /locus_tag="PLZ74_04870"
     CDS             complement(11721..12116)
                     /locus_tag="PLZ74_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97688.1"
                     /translation="MRKNRKRHARAKVLPPSVVGFFVLLVSMILCYWFMDSKCAQLGQ
                     EIRKHEQKFSALENERVREEARWNAKKTPEKLERAMLQHGLDMNYPTATQVVRMDAAT
                     GAPVPGQLSVIAFRRSRNAGERVAGTVRK"
     gene            complement(12131..13087)
                     /gene="rsmH"
                     /locus_tag="PLZ74_04875"
     CDS             complement(12131..13087)
                     /gene="rsmH"
                     /locus_tag="PLZ74_04875"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOR97689.1"
                     /translation="MHLSVLPREVLETLEVIPGGSYIDGTLGAAGHACAILKRAGPGG
                     RLLGIDRDPEALERSAARLSALSGETVLAHGTHGEVRRLAEANGFAAVNGMLLDLGVS
                     SEQLDTPGRGFSFRLDGPLDMRMDPTRGESAAEMLARLDVQSLADVLRRLGEEPQARR
                     VARAICRAQAEAPIVTTGRLAEVVGKALGGRKGPRHPATKTFQALRMAVNREVEELAQ
                     ALEDGLQLLAPGGRLVVITFESVTDRLVKQRFAAHIGRWVSLQQGGERWEGELPPARA
                     VTRRAVTPGAEEIAANPRARSAKLRAIERCERPERVRGKGFC"
     gene            complement(13233..13712)
                     /locus_tag="PLZ74_04880"
     CDS             complement(13233..13712)
                     /locus_tag="PLZ74_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97690.1"
                     /translation="MAFVGGQSGGGASGPGLLVGRFDHALDPKRRLTVPAGWRDLMGS
                     PAYVYVFPDPNETCLNLIPPAEMEGRLEKLRQRALFDKSASSALRVFGENAEQAVLDV
                     QGRIRIRDRLLAFAGLKDTVVMIGAMNRIQLWSPALRPDTETVDQAALAQACETLAF"
BASE COUNT         2685 a         4997 c         3785 g         2424 t
ORIGIN      
        1 aactctcaag aaaaatccgt cgcgcgccga cagccacgaa aggccgcggc gcgtttcttt
       61 cgcgttcgca ttccgacaca agcttcctca tcttcttccc gcatcccctc cggatcggca
      121 tccgtcgggt ctgcgcctga gcttccggat atcatagatc aatacgccga ccggatgtcc
      181 ttccggttgt tggcgcggat gggaatagta gcagaatgaa ccgcacgatg tcaacccgtg
      241 cggcgaagaa aaatcacttt ttttcgctgc ctatccggtc cgcctcacgc ccagccgcgc
      301 aggcgaaccg cctcggccac ccggttgatc gcgatcatgt acgcggcgtc gcgcaggtag
      361 agtttcttac ggcgggccag atcactgacc gccgcgaacg cggaagtcat tttcgtgtcc
      421 agtttagcaa gcacctcatc cttctcccag aagtagttgg tgttgcactg cacctgctca
      481 aaataactgc aagtgacccc gcccgcgttg gtcagaaagt ccggcaacac aaagatgccg
      541 cgttgttgga gcacctcatc cgcctccggt gtggtcggcc cgttcgcgcc ttcggcgatg
      601 acccggacgc tcttcgagat cttctggacc gtgtcgcggt tgacctggtt ctccagcgcc
      661 gcgggaatca gaatatccac ttcctgttcc agccaggctc cacccggcag catctcatac
      721 ccgagttcac gcgcgcggtt cttgtcgatc ccgccaaact tgtcagaaat gccgcgcaac
      781 tccttgaggc agaccccgtc cgccttgcgg aaggtgtagg aggtcttgtc cgtctggtcc
      841 caacaggaga tcgcgacaac cgtaccgccg agttcggtgt acaactgcac ggcgtgttgc
      901 gccacgttcc cgaatccctg cacgctggca gtggtcttcc gcaattcgat gccgagttcc
      961 cgtaacgcct cgcgcaacgt gaacaccaca ccgaagcccg tggcctccgt gcgcccgagc
     1021 gacccgccca tgccgaccgg cttgcccgtg atgaagccgg ggtaacggcc gccgcgaatc
     1081 gtctcgaact cgtcgagcat ccagagcatg tgctgggcgt tggtcatcac atccggagcg
     1141 ggcacgtcgc tcaccggtcc gagattccta acgacctgac ggatccaccc gcggcagatc
     1201 tgctcctgct ctcgcagact caggtggtgc ggatcgcaaa ccaccccgcc cttgccgcct
     1261 cccagcggaa tatccaccac cgaacacttc cacgtcatcc acatcgacag cgcgcgcacc
     1321 gtatcgaccg tctccattgg atgaaaccgg atgccgcctt tcgcaggccc ccgcgcatca
     1381 ttgtgctgaa cccggaaacc cttaaacacg cgcacggtcc cgtcatccat ccgaaccgga
     1441 atggagaact ggtattcccg catcggctga cgcaacagat cacgcacacc cccgtctaat
     1501 tccagtaccg cagccacctt atcaaactgc gattgcgcca cctgaaacgg gttatacgaa
     1561 ctactctcca tactctatct cctcttcttc acacatcgga caccacgagt cgaactgacc
     1621 gtgaacggtt cggatgcgtg aggctgacgt tatactaaaa cgccgccctt acgacaatga
     1681 cgacttcacg gtatattctt cccggccgtc gccgaacgcc acaccgggat cctcagcacg
     1741 cgtatgcggt ccggttctcc cgtggccaca agaccggccc cgactgacca acggaaacgc
     1801 cgcctttccc cttgcccctg agcgtgccac gaggccacca acggaatctg ccagaatgtg
     1861 aagtctgaac ggcggtcccc cacatggcgc agcaacccga aggccgtgcc ccaatagtgc
     1921 cggtccggcg tgtgttcaac gcgtaacagc cccccgcagg cgaggcggac atgaaagaac
     1981 cccgtgccgc cgagcaactc cgcggcccac ccgcccaaca ctccgcaccc ggaggggccg
     2041 acgctcaccg gactaggcag atcctcccac cacgaggcaa accatgacgg cgtttccgct
     2101 tccacccgcg acgccagggc acgcgatgcc ttctccccgc acaccgcctc ctccgcgccg
     2161 ggaccgctca tccaaaccgc cgccaccaaa cgcccctcgc gccgcacggc atgaagcacg
     2221 cctgtaaacg cgtcggattc cagccgtcgt aacacctgtt tccggaacgc gcgcgcatgc
     2281 cccggtcgct cccaatagct cagccagatc gtgcatgttg tcccgtccgg cgcttccgcg
     2341 agaaagacac ggtcgcccaa ccacccctgc agaaagccga aatcccggac cgacgcgagc
     2401 cgttccccgc ctaaccacaa cgccgtcccc aaaacaccga gccggttggt cgaaaccggt
     2461 tgcatcacat ccgggccctg tcccatcaca tcagacgcga gcgagatctc cgcaggctgg
     2521 aacggccgca ggtaacgccg ttccggatag aggatctcgg cggtcgtgac cggcaggcgt
     2581 tcgcggtaaa cccggtccaa cgcccgccat cccgcctcgc gccgcaaacg attcgaaaaa
     2641 acggcaccgt ccagatacgg ccgcaccagt gccccggcca gaaacgccgg gtaggcgtcc
     2701 agaccggcac caaccgtatc cgtgagttcc agccggctga caagcgaaac cagaaaagcc
     2761 cccaagggtg ttgcggtgtt gaccggccat gccgcgcaca gattgtccgc accgaccagc
     2821 accgcgttac cctcgaccag tgaagtcaag gcgaggttgg cgtccaagtc gctgagtgcc
     2881 gcccgggtca accgctcaag gtcgaaatgc acatcctcga ccgcgtggca atactcatgc
     2941 gtgtacacga agcggtcgac cagcgcccgg tttgccgcgc cctccggata gccggacccc
     3001 ggcacctcaa cgactgcgat acgttttttc tcgggatcgt aataggcggc cacctgctcg
     3061 cgcatcagcg cgcggtagag cgcgcgcagc gacacatcgg ccgggaggac acggaaatgc
     3121 cgtgcgagcc gctcggtctc ttccagaaac cgccggttct ccggtttatc gagttcggcc
     3181 tccagcaccg ccatccagac gtcgtgcgcc tcactcgtca ccgggatctc gcgtgtcaac
     3241 ggcagaccgc gcaaccgcgc ggcctcctcc ccgtagcaac gtatcggcag cggacccgat
     3301 tccggctcgc gcaacgcgcg gcaccctcct gctgcggccc acagtgccgc cagcaggatt
     3361 ccccgcgcca tgacgcgccg cacattccgt cccggcactc ttcccccctt tgaattcccc
     3421 cgaccggaac cccgcagtcc cgccttacac ctcttgcgcg gcgttacgca ccacctgcgt
     3481 atgcatgcgc tcgtcaaaca catccacatg gcgccccacg ttcaacagcg tgccgaccgc
     3541 aaacaacgcc gtgatgagat tggtgccccc atagctcatg aaaggcaggg ccagtccttt
     3601 ggtgggcagg caccctgtga ccacgccgat gttgaacgcc gcctgaaaca ccagcaggag
     3661 cgtcatgccg aacgccagca tccggccgag ccggtccgcc gcacgtaacg cgatccggat
     3721 gccgcagaac aggatcacgg tatacgccgc caccacgaac agggaaaacg ccagccccaa
     3781 ctcctcgccg ccgatcgcga agatgaagtc ggtgtgggcc tccggcagat agttatattt
     3841 ctgaatactc tgattgaacc ccacgccgga cggaccaccg ttcttaaagg ccagaatcgc
     3901 ctgctccaac tgatatttgg cggcggaggc accctcctcc gtgtgtccgg gcggcaacca
     3961 tctctcgatt atcaaacgga actgttcggg gagccaggcc gcgatgcggc gcatgcggtt
     4021 agcgtttgtg accagaaaag cgcccacccc cgccagaccg agtccgccga acagcagcag
     4081 gtacacgaat cgcgtaccgg ccacgtacat cagcgccccc cccaccacgc acaccaccag
     4141 cgtggagccg aagtccggct ccatcaccag caaaaccaca agcaatgcca atccgcatcc
     4201 gggcaagagc gcgccttggg tgaattgacg cacccgccac ccgatgcggt ccatccagac
     4261 cgatatccca atgaccgtca ggattttggc gaactcgcta ggctgcatgc ggaacgggcc
     4321 gaggttgagc caacggtagc tgcccttgac cttgccgccg ataccgggga cgaagaccag
     4381 caacaacgcc agcaccacca gcagataaaa gccgacggtc agcaacggcg tattccgcca
     4441 ccaatgataa tcgaagtggt tgatcaaaaa caccgtcacc gccgcgagac cgagccagat
     4501 cagttggcgc cggacgaaaa agtgcgggtc agcgtataaa ccctgagccc gcaccgcact
     4561 agccgtagcc aacacgacaa ttcccaaccc cagcaacgcg accactgtca acgacaagac
     4621 cgtcagagtg ctgcgcatgg gatgccgtcc gtctccgtta ctgtcccgcg ttcgggatct
     4681 tcaacacggt cccggccttc aattcgcttg aggtgaggtt gttgagcgcc ttgagatcgc
     4741 tcgggctcac accccagcgg atcgccacag cgtacagatc ctcaccctct ttcacggtgt
     4801 aggtctgcgt ggaagactcc gcagccggag cggccggcgg cggcgcggtc tcgattttca
     4861 ccggagcgga ttcaaccgcg gcgggcgccg ttgttttctc ggcagcgggc ggcggcgtgg
     4921 tcgtcacggc cggggccggt tctgtcttga cggcggccga cggtttcggc gcgacggttt
     4981 tgggagcctg caccgaagcg gcgctcttct tcgtcaccgg tgcggcggac ggtgtcttga
     5041 cggcaccggg caccttcagc ttctgaccga cgatgatttt gtcgctcttc aacccgttgg
     5101 cctcgcgcaa ggccgcagtc ttgattccgt gtttgtgcgc gatgaccgag agagagtcgc
     5161 cgggcttcac cacatacgtg atcgtcggac ccggcacggc tgcggccgct ttcggcgcca
     5221 ccgtcttggg agccctgacg ccgggtgtcg ccaccggcac ggagcgcgcc ctggagaggt
     5281 ccacctgacc ggggagctgg atcacctgtc cgatgcgcag ccggttaggc acgatgccgg
     5341 ggttgatggc cacgatgtcc tgccagcgca cattgtactt gtacgcgatg gcggaaaggg
     5401 tgtcgccctt ctgaatcgtg tagggggtcg aagtctgcac cgacggcaac tcgaccaacg
     5461 aggtttcgac aggttgaaca ggcgggggag ttgtatccaa cggctgcacc tccaccggtg
     5521 gcagttcccc gtcaggcaga gtggtcgaag tggtgtcact cttccggtta aacataccgc
     5581 agccctgcat caggaagcaa cccacaatca ggttgacgcc tgcaacaaca cgcatgactt
     5641 gacgattcat tgtgtctcct tatccttctt gcttgccctc agaaaagaaa cgctcagaga
     5701 tccgcaaccg cgcgaacctg tgccgtgaaa cgttctcccc tttcccgata gcttgaaaac
     5761 tggtcgaaac tcgctgtccc cggcgaaagc agcacggtgt cacccgcccg ggcttcacgc
     5821 gccgccgccg ccacagcctg atccagggtc ccgcactctt cgcaagggat cacatccgac
     5881 caggcctgaa acatctgcct tgaacagtgc ccaataagat aacctttttt tgtggttttt
     5941 gtcagcaact cttttatccc ttcgagatcg ggctctttta aaagcccgcc cgcgatcagg
     6001 cgtaccggtt tgcgcgccat tctcagtgcc gcggccgtgg ccgcaagact ggtcgctttg
     6061 gagtcatcca catagcggac gccgtgtcgg ctctcccaca cgagttgcat acggtgcggc
     6121 agcggctcga acgcccgcac tcccgccgcg atctgctccg catccagccc tgcggcatgc
     6181 aatgccgcca cgcccgccga cagcgaaagt ccgaacactt cgttgtcgaa ccacgacccc
     6241 ccggtctcca cccggacacg cgtaccctcc gcctgccccg tcaccgcatg atccgcataa
     6301 cgccaatcgc atcccacatc cgtaccgaac cgccacagcg tgacccccgg aggcacgcac
     6361 ccgtcgtcct cgaacccggc cggcagcagc acgacatccc ccggacacat cgacgcaaag
     6421 agccgcattt tggcgctctt gtattccgtc atgtccacat gccgatcctg atggtcggcc
     6481 tgaagattca gcaggatcgc gatctccgga tgaaaggtcc gggtatgctc catctgaaat
     6541 gaactgacct cggtcaccgc ccagagcagg tcgggccggt cgagcgcgag ttggcacaac
     6601 gggatcccgt aattgcccgc aggcgaggcg gggatccccg cgcccgtcag cgtgtccgaa
     6661 caccacttga ccaacgaact tttgcctttg cttccggtta tggcgatcac gcgcccggac
     6721 cagtattcgc tcccgagttc cagttcggac accatcggca atccgcgcgc acggcatgcg
     6781 gtaatccacg gatggttcag ggcgaatgcg ggactcacga cacacacgtc gaacggttca
     6841 gacggcagac cggcggcccc gaacgtcacc tgacctccgg ctgcggtgat ccgttgcgcc
     6901 actgcggcgc ttgcgtccgc tttcccctcg tcaatcaccc gcacacgccc gccgcgggcg
     6961 agcaacagtc tcgcggccgc ctcaccgctg attcccgcgc ccagcaccag agcctgtaca
     7021 ccgtcatact tgctcatggc ccacaatccc ttccagccgc atgccgcgcg acgctttgag
     7081 caaaacactg tcaccgctcc gtatccactc gcacaacacg atacccgctg cccgcgcatc
     7141 gtccgcacag accaccttcg tctgcggaaa acccgcccgc accgcctcct ccgccaaata
     7201 cagcttcgcg tcgtgaccca ctgtcaacag tctgtccgga gccgtctgtg ccacacaacg
     7261 gccgacctcg cgatgtaacg cctcggtgtg ttcacccagt tccagcatat cgcccaacac
     7321 cacgatgcgc cgtccccggc agggcagttc cgcaaacgtt ttgagtgccg cggccatgct
     7381 ctggggattg gcgttataag catcgttgac ccacgtcacg ccatcgcgca ccatcacctc
     7441 ccagcgcatc ttgggcagcg tcagacccga cagggcctgc gccaacgcgc aggcgggcag
     7501 tccggcacgc cgcgccgccg cgaaagccag cagcagattc gacgcattgt gcgcacccgg
     7561 cagtccgctg catagtcgcg tctcttcgcc ggtcgcctgt tccgtgattc gcagcgcgcc
     7621 ggtctggaga tccgtcgcct cggccgagaa atccgccccc cctcccgtca gcgtaaccgt
     7681 caccaccggc gcgccggact gttccgccag ttgcgcaaat cccgtaatct cccggttcag
     7741 gacggcgaac cccccgtgcg gcaacgcgcg caacagcgcc cccttctccc gcacgatcgc
     7801 ctcctgcgtg ccgaagtgtt caatgtgcgc gcatccgatg ctggtgatca ccgccgcgca
     7861 tggttgaagt gtgtcactca gccgcccgat ctccccggga tggttggagc ccaactcaaa
     7921 gatgccgtac tcggtttccg gcggcatggc gagcagactc agcggcaacc ccacctcgtt
     7981 gttcagattc ccacgtgtgg cgcagacgcg cccgcctgct gcaaacagcg cggccgccat
     8041 ctctttggtc gtggttttgc cagaactgcc ggtgatgccc aaaatgaacg cgcggttcaa
     8101 acgccgccac gcgcgcgcgg catcagccaa cgcttggcgg gtgtccttca cgcgaatcag
     8161 gggccagccg gacgatccgg acggcgcgga ccaggccgtc tcgaccaacg cggccgcggc
     8221 accgtcgcgc aaggcctgtg ccacaaaatc atgcccgtca aaccgttccc cgcgcaacgc
     8281 cacatacagg cagcctggcg ttatcctgcg cgaatcctgc gtcacgccgc agaacgttga
     8341 cggcaacgcc tgcgcgtcac cgcccgtcca tgcggcaaac gcggccgcat ccaacctctg
     8401 tggctgcttg ttcgacatct tcgtgcccgt cccgattgtc caccctaccg atcctgctgt
     8461 cgcgcctcgc tgagtattcg cgcctcctcc gccaccacct gccggtcatc aaagggcagc
     8521 gtgcggcccg cgagttcctg aaatgactca tgccctttgc cggcgataag caccacatcc
     8581 ccctcgcgcg cctgactcaa cgcgcgtcgg atggcaagcc ggcggtctgc ctcaacctcg
     8641 tacgaggtcc cttccggtat gccctgcaga atttcctcaa tgatcgcccg cggatcctcc
     8701 gaacgcggat tgtcggaggt gatcaccaga tcatccgcca gagcggcgca cgcgcgcccc
     8761 atcagcggac gcttggcacg gtcgcgttcg ccgccgcatc cgaagaccac cctcaggcgt
     8821 ccgcgcgtca cctcgcgcaa ggtctccaga acattcgtga gcgcgtcatc ggtatgggca
     8881 tagtccacaa acaccgtggc gggcagatcg accggcacac gttccagccg gccccaacac
     8941 ggccgggcgg cctccaacgt ctctcccacc ttctccaacg gcactccggc ttccaacgcc
     9001 aaaccggccg cgcacagcat gttggctacg ttgtaccgac ccgcgaggca cacgcgtaat
     9061 gcaaggcggc gtccatccgg tgccgccaat gtgaacgtac tggcgtcggg ggtaacggcc
     9121 agggcttcgg cccgcagatc ggccgcttgc gtgaacccgc aggtgaggcg ccgtaacggt
     9181 agcgtggcga gataggcggt catgcgatcc ccatatccgg catctgccac gcacacggcg
     9241 agcgcacccg gcgtttcagc ggccaattgg tcgaaaagct tgcacttggc gacgaaatac
     9301 gcctccatcg tgccgtggta atcgagatga tcccgcgaca gattggtgaa ggccgcgccc
     9361 gcgaaccgtg tccaggccac acgctgctga tccagcgcgt gcgacgacac ctccatcact
     9421 gcggtgtcac aaccactcgt gcgcatggcg gccaaaatgg cctgcaactc acaagcgtcc
     9481 ggtgtggtgc gcgtggcagg gatcgcgcga ccggcatact cgacctgaac ggtggtcagc
     9541 aatccgggca cggcccctcc ggccaccaac atttcgcgca acagccacgc ggtggtggtt
     9601 ttgccgttcg tcccggtaat cccgtacacc cgcatgttac gggacggcca cgctttcagc
     9661 gccgacgcga tacaggccaa cgcgaggcgc acgtcccgga ccagaatgac ctggcggtga
     9721 tactccggca gcggcgtttc cgaaaggatc gccaccgcgc ctttcgccag cgcggcaccg
     9781 gcgtaacgtc cgcccgcatc acgcgtgccg gtgatcgcag caaacaaatc accgggccgg
     9841 atccggcgcg aatcgcactg gattccggtg aacgccacgg tcggtgcctc ggaaacgagt
     9901 aggggcgcga gttctggaat ttccgaaatt ttcatgaaaa cttctccacg gcgcaagttc
     9961 gtgccgtctg ctacgggtga ttcgccactt gctccgtttc ctcggcaggc tggtcgggag
    10021 ggacttcgag ataccgggcg gtcgccgccg cgatcttggc gaaagcgggc gccgaaacaa
    10081 agcccccggt gtgctggggc cgcggccgct ccacagaaac cagcacgctg aagaccggat
    10141 cccctgcggg tacgaacccc acgaacgaag cgtaataatc cgtgctggag tatccgcccg
    10201 gcaccgcctt ctgcgccgtg ccggttttac ccccgatcga atatcccggc acggaggccc
    10261 gtttccccgt gcccccctcc tcggtaaccc cgcgcaacat ctcgcgtaca gcgcgcgcga
    10321 cctcgggacg caccggccgc cccaacactt cgggcttggc ctcaaaaatc gtctcgccgc
    10381 tgggagagac gatccgccgc accacatacg gcttcatcat cacgccgtca ttggccagcg
    10441 tcgaataggc tgagatcatc tgcaatccgg taacggccac accctgaccg atcgggatac
    10501 gcgtgggttt gagtttgtcc caatcacggg cccgggcgag aataccccgt tcctcacccg
    10561 gcagctcgat tcccagtttt tcaccgaaat tgaaggcccg cagataggtc tcgagccgtt
    10621 tgttgcccag catcaaaccg attttcgcgc acgcgatgtt gctcgatttt ttcagggcgg
    10681 tgctgacggt gacccgtccg tgaacatgat cacgcaacgg tttgcccgcg tacatccaac
    10741 acccctcccc cgcatcaatc actgtttccg gcgtcacgat gcgttcgttg agcgccgccg
    10801 ccaccgtgat cgccttcatg atcgaacccg gctcgtagac gattgccaga gccgagttgc
    10861 gccagacatc gacactctcc tcggtatact gttcgggcgc gaaatcgggg aaggaggcca
    10921 tggccaaaat ctcgccggtg cggacccgtt gcacaatgac ccacccgccc gcggcctcaa
    10981 acttctccac cacctcgcgg agcgccaact cgacctcata ttggatgtta tgatccagcg
    11041 tcagatagag gtccgagccc gcaatagccg ggacatgcac cgtgcgccga ctccagattt
    11101 cgcggcggcc ggcatccttc tcgccctcga tcaagccggg tgtgccggtc aggtaacggt
    11161 tgtactgctg ctcgatcccg gcaccgccca cccccatagc attgacgaac cccagaacgt
    11221 gcgacatccg gcgaccctgc ggataggagc ggcagaccag atcttcggaa ccgatcccgc
    11281 tgatcgtctc cggatccgcg agcaggtcga acaccttgtc gttgagcgaa acccccagct
    11341 tgatgtaccg cgagtcggtg cgggcaaatt gcgcgtatac ctcatccgct tccagaccca
    11401 gacacgccgc gaccgtctcc gcgatctgtt ttttgtcgtg ctgcggtttg accgacttcg
    11461 gatccaaaaa gaactggcgc gccggcagac tgaccgccat gggaaagcgg tcgccgttgc
    11521 agtcaaagat acggccgcgt aaccccagca gtgtccgccg gtactcgcgc gtcagtttgg
    11581 aatggttcgc gagatggaga taggccagcc gtgaagccag tcccaaaagc gcaaaaaaca
    11641 acgcggcggt caccagcaga atacgcatct gaatgccgcg gtcatgcatg accaaccctc
    11701 cgtcccggaa tcaagtccgc ctacttccgg actgttcccg ccacgcgctc gcccgcattt
    11761 cggcttcggc ggaacgcgat aaccgagagt tgcccgggaa ccggcgcacc ggtcgccgca
    11821 tccatgcgca ccacttgcgt ggcagtcggg taattcatgt cgaggccatg ctgcagcatg
    11881 gcccgctcca gtttttccgg cgtcttcttg gcattccaac gcgcttcttc gcgaaccctt
    11941 tcattctcca aggcggaaaa cttctgctcg tgttttcgga tctcctgacc gagctgggcg
    12001 cacttggagt ccatgaacca gtaacaaaga atcatgctga ccagcagcac gaaaaaccct
    12061 accaccgacg gtggcagcac tttggcccgc gcatgccgtt tccgattttt tctcatcttc
    12121 caatccctcc tcaacaaaaa cctttgcctc gcacccgttc cggccgttcg cagcgctcaa
    12181 tcgcccgcag tttggccgac cgggcgcgcg gattcgccgc gatctcttcc gcgccgggcg
    12241 tgaccgcacg ccgcgtcacg gcccgtgcgg gcggcagttc gccttcccag cgctcaccgc
    12301 cctgctgcag cgaaacccac cggccgatgt gcgcggcgaa acgctgcttc accagacggt
    12361 cggtcacgct ctcaaacgtg atcaccacca gccgcccgcc cggcgcgagc aactgcaagc
    12421 cgtcttccaa tgcctgcgcc aactcctcca cctcgcggtt cacggccatc cgcaaggcct
    12481 gaaacgtctt ggtcgcggga tgccgcggcc ccttacgccc gcccagcgcc ttgccgacca
    12541 cctcagccag ccgaccggtc gtcacgatcg gcgcctccgc ttgcgcgcgg cagattgctc
    12601 gcgcgacccg ccgcgcctgc ggctcctcac cgagccgccg caacacgtct gcgagagact
    12661 gcacatccag ccttgcgagc atttcggccg ccgactcccc ccgagtcgga tccatgcgca
    12721 tatccagggg cccatccaac cggaagctga acccccgtcc gggcgtgtcg agctgttcgg
    12781 atgacacgcc cagatccagc agcatgccgt tcactgccgc aaacccattc gcctctgcca
    12841 ggcgccggac ctcaccatgc gtcccgtgcg ccagcaccgt ttcgccggac aacgccgaca
    12901 gccgcgccgc agatcgttcc aaagcctcag ggtcccggtc gatccccagc agtcggccgc
    12961 ccggccccgc acgcttcagt atcgcgcatg cgtgacccgc cgcaccgagc gttccgtcga
    13021 tgtagctgcc gccgggaatg acctccaggg tctccaggac ttcgcgcggc aacaccgaca
    13081 ggtgcatagt ccctccagac acatccttgt caataatgat cgcgcggcaa cacacgacgc
    13141 tcccgaatcc gcgtccgtct tccggacccc gtatcccgcc cgcgatccaa atcccttgtc
    13201 aggccgcgcc tcgctgccac gcggcgaact ttctaaaaag ccagggtttc acaggcctgc
    13261 gccaacgccg cctgatcaac cgtctcggta tccggccgca acgcaggcga ccagagttga
    13321 atccggttca tggcaccgat catcactacc gtatccttca accccgcaaa cgccagcagc
    13381 cgatcccgaa tgcggatccg cccctgcacg tccagtaccg cctgttccgc attctcgccg
    13441 aaaacccgca acgccgaact cgcgctcttg tcgaacaacg cccgctgccg cagtttctcc
    13501 aaccgcccct ccatctccgc aggcgggatc aggttcaggc acgtctcgtt cggatccgga
    13561 aaaacataaa cgtaggccgg cgaccccatc agatccctcc agcccgccgg tacggtcaga
    13621 cggcgcttcg ggtccaaagc atggtcaaac cgcccgacca gcaaacccgg cccggaagct
    13681 ccgcccccac tctgtccgcc aacaaacgcc ataccttgac cttttgtgac atcattcaac
    13741 actttccccc accagaagtc aacccgaaat ccctgaaaaa atattcgggg taaatagcct
    13801 taaaagggtt tcagggtttc tggtttcaag gtttcagggt ttcagggttt ctgggtttca
    13861 tccaaatcgg ggtcgggatc gctatcggga t
//
LOCUS       DAOSRB010000061        13745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4553_length_13745_cov_7.758510, whole genome shotgun sequence.
ACCESSION   DAOSRB010000061 DAOSRB010000000
VERSION     DAOSRB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13745
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4553_length_13745_cov_7.758510"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            519..608
                     /locus_tag="PLZ74_04885"
     tRNA            519..608
                     /locus_tag="PLZ74_04885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:552..554,aa:Leu,seq:taa)
     gene            complement(649..1530)
                     /gene="rfbA"
                     /locus_tag="PLZ74_04890"
     CDS             complement(649..1530)
                     /gene="rfbA"
                     /locus_tag="PLZ74_04890"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOR97691.1"
                     /translation="MARKGIILAGGAGTRLFPVTQVICKQLLPVYDKPMIYYPLSTLM
                     LAGIREVLIISTPEATPVFRHLLGDGSQLGMRLCYQVQETPNGLAQAFVIGREFIGDD
                     PVCLVLGDNLFYGDGMRRMLAEADSGAGATVFGYYVTDPERYGVVSFDAAGRAETIEE
                     KPAQPKSNYAVVGLYFYDQDVVQIATELKPSARGEYEITDVNRVYLQQGRLNVKVMGR
                     GVAWLDTGTHDSLADATAFVKAIQDRQGLKIACIEEIAWRMGFIDRAQLERLAASLSK
                     STYGEYLAHLLKQEIRS"
     gene            complement(1586..2662)
                     /gene="rfbB"
                     /locus_tag="PLZ74_04895"
     CDS             complement(1586..2662)
                     /gene="rfbB"
                     /locus_tag="PLZ74_04895"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOR97692.1"
                     /translation="MKRVIVTGGAGFIGSAVIRHLLDATDWQILNLDKLTYAGNLASL
                     PGAEDNPRYAFLRADICDRAAMEQAFRKFRPDAVMHLAAESHVDRSIDGPAAFVQTNI
                     VGTATLLEVALTYWRGLSDAEREAFRFQHISTDEVFGSLEPVGLFTETTPYDPRSPYS
                     ASKASSDHLVMAWHHTYGLPVMITNCSNNYGPFHFPEKLIPLVILNALEGKPLPIYGK
                     GDNVRDWLYVEDHARALRRVLERGVPGETYNVGGNTERTNLQVVETICATLDETCPER
                     LSGFGIQAFRELITFVPDRPGHDHRYAIDASKIRDRLGWAPQENFETGIRKTVAWYLE
                     NAWWWQPIRDKNYAGERLGRGTGS"
     gene            complement(2672..5116)
                     /locus_tag="PLZ74_04900"
     CDS             complement(2672..5116)
                     /locus_tag="PLZ74_04900"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOR97693.1"
                     /translation="MNEREDTTAGDVSTGTRRPIYYGGFNKRAIYYGGHAPSAYPGYP
                     GGGYGGGAYYYGMGGGVGDDTDSLMGTVTIGRMLRVCSQRWVTLVVFVIIGFIAAFAV
                     FKISPMIYEAESVFEMSIRGSTYTGMRKAIIETDVGSSLDEVFNTRLARLRSRAVIDQ
                     IVSQYRSDYPSSTVTDEQLIETLFESKMTLQRRSRLIRVTVRSTDPQLATDLANAYAK
                     AAETFTSDSNRNESEVAVSWLATTTEQQKRNLERADKEMLDFRVANQLDFMASESETT
                     QQARGKINADILVLEGEITKATELRKTLEQIQNEPEKFGTLPEAVPRSGEIGTAYQQL
                     QNVLAEKNSLLARYTANHPEVKVKEKEAEVFKQQFADVVFRALETCKANLDLLQRQLA
                     QLTPKRDELVKKLSDLELKMVETKMKLEQLEREREVADISYKSLLQRTREAQVASDEN
                     TAIIKQVEAAYRPTKPVLPNPMIIFPAGPLIGLLLGVVFVLVLDHLEDKIVGISDIEQ
                     RLRLKTLAVLPHVRRKKREQLARLVADDKFSQFAEAVAGLRNLLDSPRYQEMTKVLLC
                     MSTQPGEGKTIASCSLALSYAQSGQKTLLIDFDMRRPRLARIFEKRHSDFNSLPHTLA
                     KGDTSLFETLPVASGMENLDVVLSRASSELSPSSLMGSGVIVDFFSWAREHYDHVIID
                     SPPFGIVGDVMVLASLVDAVMIMCCPDRTRFQPIKHAARHLTEAGARVIGVIVNDVDF
                     GRRNHFSHYDYHYRYAYRYTSRYGAYGQGKKPGDRTRAAAAGGAGTVRLDEDDLIPSG
                     LAARRDVVDVSMTDDE"
     gene            complement(5177..6583)
                     /locus_tag="PLZ74_04905"
     CDS             complement(5177..6583)
                     /locus_tag="PLZ74_04905"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOR97694.1"
                     /translation="MMQKILTKYGLAIHVALVCFFPFAGFGSSRWLPFIPFLWLSLAV
                     VELMILLPSVYRGETLADARQRMVRGLYWDPLLYIGAALVGMLMIQWLNGGCPLVYLP
                     DADVWRISTPPIAWAPFCVEAVAGFSQFCVLVACVVVGLAVRNALSRTPKRMLLQGMA
                     VASGCVALISVARVWPERCLADAANLARGTFFGFWMLLSWGLLADALTRFQRGGRTLY
                     LFGTLANLIGLLFYSPPLSVCLYGILGLALFLYALVYLDAQVSKKTQVLLFLVTVFAL
                     AALVVTLGFLMPDNPVAAKMKEAWPVAEYWEKLMATRRVRMDAALALWKEHLWFGCGA
                     GGFFHGVGLLLSDKEWALLQTDQAYAYHEYLQFLCEYGVVGLALFLTAALALIAPLCY
                     RARIAWQAGGEVGMEGRFFLLRISPIVVSGVLATGAVFVESWFANPFRSPAVLLSWSV
                     ALAALPGFLPKHANKKAR"
     gene            complement(6682..7884)
                     /locus_tag="PLZ74_04910"
     CDS             complement(6682..7884)
                     /locus_tag="PLZ74_04910"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOR97695.1"
                     /translation="MVTRTVFVARARIILMFALILVCGVTRAQERGFKFNNLTVSPYV
                     NLEYMYDSNVDSDRTEVSDEIFSVNPGVDLTYTGNDWGLSAGLWFGRDYYRDYDELDK
                     NRYGERVSFYRESAKGWRFVLGQSFIRSRENDSILDGGRGLWRDRDLHEINSALSYQI
                     SEKTGVTLSGMFSDLNYRNDQGRYAPLYGWREWSTGLEFARKLSEKSNVLLAGTYQQY
                     KSNGAVGGVDSSSSGYSLMAGFGSRATERISYRALTGVEWFDYAEGDLLTGWKYSLDA
                     SWLISKKWAATVAGSSYYQPSETQVNQAMQVHAISAGVTYRPMRKLTTRLDVAYRREE
                     DEYQNAVARNMGAATDDIFSIRARADYQLMRYVSVYGGLEFEDRSSDNRAYDYDQYRA
                     LLGLQFRY"
     gene            complement(7935..8933)
                     /locus_tag="PLZ74_04915"
     CDS             complement(7935..8933)
                     /locus_tag="PLZ74_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97696.1"
                     /translation="MKKSKRVLGCALGVGLAWAAVVGLAQQSEFLSRSQWLKKIGASV
                     TDAAVLRDTLEQVAPSDRVEFTQRLLKAVSRLPANPDEKGAAFVRSAVACIAGADAET
                     KRDVIAAVFAGVPVEYLPLVTDELAKRFNREYNQLTCEQYGMIATNVLGAVTVRNAET
                     DEASVRNTFTVLAFLKGADCPNLQRDLLAVLPDDRMRGLAQTWIPQVLNEGKYDAMLA
                     AADAEALPLRHEDILRLIGHASLDRLLADMLASLPADGKGASLSLEKARLGGASASSS
                     TAVSGSNLDYGINRVPRNPFYPSGYQNQKLTIIGDDFLQERGKRRVYRVKTFCGCP"
     gene            complement(8965..9615)
                     /locus_tag="PLZ74_04920"
     CDS             complement(8965..9615)
                     /locus_tag="PLZ74_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOR97697.1"
                     /translation="MLKCLGFGLLCWAVLSVNGCRTAAEDAMGFDEQAQYVEEGEPKI
                     KPGLTLRVAVTASGSEAVQEGLKEVNANGEILMPLVGAIRCEGLTIMELQEKIKTAYK
                     DYYIEPQVTVGFVYTENTGMKSPWGSVLLMGEIVRPGPVNMPSTRDLTVTRALMLAGG
                     ATALADKRHVRVTRREKDGSLKRFMVDIDKIGKEGRSDLDIALKPGDVVWVPESWY"
     gene            complement(9817..11316)
                     /locus_tag="PLZ74_04925"
     CDS             complement(9817..11316)
                     /locus_tag="PLZ74_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR97698.1"
                     /translation="MTNQRENPPMRLAIAYPPLPSEKGVPLLSQNRQFQWFTRPTYIY
                     PVVPAQAATQARAAGHDVAWLDGIASEWTPEEFDARLDAFAPEVVMLETKTPVVRRHW
                     AYIRELKARHPGVRVVLVGDHVTALPRESFEACPVDYVLTGGDYDFLLVNLLEVLRAA
                     GGGLPDTARLEPGVYWRDADGMVRDSGPFRLDHDLKTLPWIDRELTCWPLYAERNGNF
                     RKTPGTYIMSGRDCWHGKCTFCSWTTLYPTYRVRDPVEVVDEIGDLISRYGIREIMDD
                     TGSLPAGAWLKRFCGEMIARGYNRKIRIDCNMRFGCLDEETYRLMRKAGFRLVLFGLE
                     SANQATLDRLQKGLTVAQIEEGVKWAARAGLDVHVTVMFGYPWEGEGEIANTVRLARS
                     LLRRGHASTLQVTMVVPYPGTPLFRELDQAGLLLTREWDDYDMRRQVMRSGVPEARIK
                     AAVREVYQAFLHPETVVRRLLSTRDPVGDVKFYWRGLLSLVGHLRDFTK"
     gene            11449..12465
                     /gene="ruvB"
                     /locus_tag="PLZ74_04930"
     CDS             11449..12465
                     /gene="ruvB"
                     /locus_tag="PLZ74_04930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOR97699.1"
                     /translation="MQTRPSEELNRRNASFDNRLRPTGFAEFVGQQKVRERLMLAVEA
                     ARQRGEALDHVLLSGPPGLGKTTLAFILGDAMGVHVKTTSGPVIDKPGDLAGLLTSLE
                     PGDIVFIDEIHRMQRTVEEYLYSAMEDFVIDILIDQGPNARSVRLELPRFTLVGATTR
                     SGLISAPLRSRFGITNRLDYYCIEELSDILTRSAVRLEVSIDEGGTREIAARARGTPR
                     IANNLLRRVRDYAQVRAGNHITQEVAGEALALLEIDPQGLDEMDIRILETLAVKFNGG
                     PVGLNTIAVSVGEEPDTIEEVYEPFLIQEGYLERTPKGRVATPACYRRLGLTPAGRQC
                     ELPL"
     gene            complement(12809..13681)
                     /locus_tag="PLZ74_04935"
     CDS             complement(12809..13681)
                     /locus_tag="PLZ74_04935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOR97700.1"
                     /translation="MTDSAASVPCITATLPPEAADALFEIIGDDGFTPTSWYDVESGV
                     CRVSLFPDEPDGVAHTQAALLAAAALLGVTVEPTVTAVAQTDWAESWKRFFHVEKISP
                     RVVVRPSWEPYTAQPGECVITLDPGLSFGTGKHATTQACLRFLDQLAAEDIQRSVLDM
                     GCGSGILAIGAKLLGFTDVRGFDNDPDCMRVSEENAALNGVSIPFTLDDLSQAHPPAD
                     IVVANILAPVLIRFAPQVAGSMSADPRARLVLSGILDTQYDAVCAAYEAQGLVEIDSL
                     LFGEWRSGLFGRGS"
BASE COUNT         2926 a         4569 c         3651 g         2599 t
ORIGIN      
        1 tgtttccgcg ccatcattgg cgcggctcca ttgaagccct tcagttagga gttagaattt
       61 aggagttagg agttaggagt tagcccgccg cgcggctcag ccgcgaaaaa aaccttggaa
      121 taagtttagg acatgatttc cgacagattc gcttcggcga ggagaaatcc atgattaaca
      181 tgatgttgtt caatcctgaa aatcctgtaa tcctgtcaat ttatcggaca tgattttccg
      241 ctgcccgggc gcatatatcc atgattaaca tgatgttgtt caatcctgaa aatcctgtca
      301 tcctgtcaat ttatcggaca tgattttccg ctgcccgggc gcatatatcc atgattaaca
      361 tgatgttgtt caatcctgaa aatcctgtca tcctgtcaat ttataggcaa ggagttagga
      421 gtgtttcgcg cgcctttact ggcgcgaaaa gaatccaccc ggctgaggct tgacgcttct
      481 gtcatgcttc tgtatcgtgt cccgaacgat tgcgccacgc cagagtggtg gaatggcaga
      541 cacaccggac ttaaaatccg gagccgggtt aaaccggcgt gagggttcga tcccctcctc
      601 tggcaccaat attgcctccc ctgaacgtcc cggctcgggg cgcaatgctc aggatcggat
      661 ttcctgcttc agcaggtgcg ccagatattc gccgtacgtg gacttgctca gcgacgccgc
      721 caggcgctcc agttgagcgc ggtcgatgaa ccccatacgc caggcgatct cttcgataca
      781 ggcgattttc agtccctgac ggtcctgaat cgccttgacg aacgcagtgg cgtccgcaag
      841 cgagtcatgg gttccggtgt ccagccaggc gacaccgcgt cccatgactt tgacattcag
      901 ccggccctgc tgcagataga cacggttgac atccgtgatc tcgtattcgc cgcgggccga
      961 aggcttcagc tcggtcgcga tctgcaccac atcctggtca tagaaataga gtcccaccac
     1021 cgcgtaattc gatttcggct gcgcaggttt ctcttcaatg gtttccgccc ggccagccgc
     1081 gtcaaaactg acgacaccat accgctcggg atcggtcacg taatacccga acactgtggc
     1141 gcccgcgcca ctatccgctt cggcgagcat ccggcgcatc ccgtcgccgt aaaaaaggtt
     1201 gtcgcccaac accaggcaga ccggatcatc accgatgaac tcgcgtccga tcacaaacgc
     1261 ctgcgccagc ccgttcggcg tttcctgaac ctgatagcac aaccgcatgc ccagttggga
     1321 accgtcgccg agaagatgcc tgaacacggg cgtcgcttca ggcgtggaaa tgatcagcac
     1381 ctcccggatc cccgccagca tcagggtcga gaggggataa taaatcatcg gcttgtcgta
     1441 taccggaagc agttgtttgc aaatcacctg cgtgaccgga aacaaccggg ttcccgcacc
     1501 gcccgccaga ataatccctt tccgcgccat cctcccaccc tccttcccaa catgaatccg
     1561 ccgcttctcc tgaaccgaac agcggtcagg acccagtccc ccggcccaac cgttccccgg
     1621 catagttctt gtcgcggatc ggctgccacc accaggcgtt ctccaaatac caggccaccg
     1681 ttttccgtat gccggtctcg aagttctcct gaggcgccca accgagccgg tcgcggattt
     1741 tggaggcgtc gatcgcatac cgatgatcat ggccgggacg gtccgggaca aacgtgatga
     1801 gttcgcggaa agcctgaatc ccgaaaccgg acaaccgctc cgggcacgtt tcgtcgagcg
     1861 tcgcgcaaat cgtctccaca acttgcaggt tggtgcgctc ggtgttgccg cccacgttat
     1921 acgtctcgcc cggaacgccc cgctccagaa ccctacgcaa cgcgcgcgca tgatcttcca
     1981 catacagcca atcccgcacg ttatcgcctt ttccgtagat cggcaggggc tttccttcca
     2041 acgcgttcag gatcaccagc ggaatcagct tttcagggaa gtggaacggg ccgtaattat
     2101 tcgaacagtt ggtgatcatc accggcaacc cgtaggtgtg gtgccaggcc atgaccaaat
     2161 gatcggagct cgctttactg gccgaatacg gcgaacgggg gtcatacggc gtggtttccg
     2221 taaacaaccc gaccggctcc aacgatccga acacctcatc cgtggagatg tgctggaaac
     2281 gaaacgcctc ccgttccgca tcggacaggc cgcgccaata cgtcagggca acctccaaca
     2341 gggtggcggt tcccacgata ttggtttgca caaatgcggc cgggccatcg atcgagcggt
     2401 cgacatgcga ttccgcggcc agatgcatca cggcgtcggg gcggaacttt ctgaacgcct
     2461 gttccatcgc cgcgcggtcg cagatgtccg cccgcagaaa ggcataccgc ggattgtcct
     2521 ccgcccccgg cagggaagcc agattccccg cataggtcag cttgtccaga ttcaggattt
     2581 gccagtccgt cgcgtccagc aggtgccgga tcacagccga gccgataaac ccggcaccgc
     2641 cggttacaat tacccgtttc atccgttctc ctcactcatc atctgtcatg gaaacatcaa
     2701 ccacatcccg gcgcgcggcc aagccggacg ggatcagatc atcctcgtcc agccggacgg
     2761 tccccgcgcc gcctgccgcg gccgcacggg ttctgtcgcc gggtttcttg ccctgtccgt
     2821 atgcgccgta gcgcgatgtg tagcggtagg cataccggta gtgataatca taatggctga
     2881 aatggttgcg ccggccaaaa tccacgtcat tcacaatgac gccgatcacc cgcgcgccgg
     2941 cctccgtcag atgacgggcc gcatgtttga tcggctggaa ccgtgtgcgg tcgggacagc
     3001 acatgatcat caccgcatcg accagcgagg ccaagaccat gacatccccc acgatcccga
     3061 acggcggcga gtcgatgatg acgtgatcat aatgctcacg cgcccaagag aagaaatcga
     3121 cgatcacgcc gctccccatc aggctggagg gactcagctc cgaagaggcg cgagagagca
     3181 ccacatcgag attttccatc cccgacgcca cggggagggt ctcaaacaac gaggtgtctc
     3241 ccttggccag cgtatgcggc aggctgttga aatccgaatg ccgtttctcg aaaatccggg
     3301 cgagccgggg acggcgcata tcaaaatcga tcagcagggt cttctgcccg ctctgggcat
     3361 acgacaacgc cagcgagcag gaggcgatgg ttttgccctc ccccggctgc gtgctcatgc
     3421 acagcagcac cttggtcatc tcctgatagc ggggcgaatc cagcagattc cgcaatccgg
     3481 cgaccgcctc ggcgaattgc gaaaacttat catccgcaac cagccgcgcc aactgctcgc
     3541 gttttttccg gcgcacatgc ggcagcaccg ccaaggtctt caggcgcaga cgctgctcga
     3601 tatccgaaat accgacgatc ttgtcctcga ggtggtccag caccagcacg aacacaaccc
     3661 ccaacaacag gccgatcagc ggtcccgcgg ggaagatgat catcggattg ggcaacaccg
     3721 gcttagtcgg acggtaggcg gcctccacct gcttgatgat cgccgtattt tcatccgacg
     3781 ccacctgcgc ctcgcgtgta cgctgcaaca gcgatttgta actgatgtcc gcgacttccc
     3841 gctcgcgctc caactgctcg agtttcattt tggtctccac catcttcaac tcgagatctg
     3901 aaagtttctt caccaactcg tcccgtttcg gcgtcaactg ggccagttgg cgctgcagca
     3961 gatccagatt cgccttacag gtctcgagcg cacggaagac cacgtcggcg aactgctgct
     4021 tgaagacctc ggcctctttc tctttgactt tcacttccgg gtggttggcg gtatagcggg
     4081 ccaacagcga atttttttca gccagcacgt tctgcaactg ctgataagcc gtgccgatct
     4141 cgcccgaccg cggcaccgct tccggcaggg taccgaactt ttccggctcg ttctgaatct
     4201 gttccagagt tttccgcagt tctgtggcct tggtgatttc cccttccagc accagaatgt
     4261 ccgcgttgat tttgccgcgc gcctgctgcg tggtctcact ctcgctggcc atgaaatcaa
     4321 gctggttggc cacccggaaa tccagcatct ccttatcggc ccgctccaag ttccgcttct
     4381 gctgctccgt cgttgtcgcc agccacgaga cggccacctc cgactcgttg cggttcgaat
     4441 ccgacgtgaa ggtttccgca gccttggcat aggcgttggc gagatccgtc gccaactgcg
     4501 gatccgttga ccgcaccgtg acccggatca gacgcgaacg gcgctgcagc gtcattttcg
     4561 actcgaacaa cgtttcgatc aattgctcat ccgtgaccgt cgaactgggg taatcactcc
     4621 ggtactgact gacgatctga tcaatcaccg cgcggctgcg caaccgggcc aaccgcgtat
     4681 tgaacacctc atccagcgac gaaccgacat cggtttcaat gatcgccttg cgcatgccgg
     4741 tatacgtcga cccgcggatg ctcatttcga acacactttc cgcctcataa atcatcggag
     4801 agatcttaaa taccgcaaac gcggcgatga agccgatgat cacaaacacc accagcgtaa
     4861 cccaccgctg cgagcacacg cgaagcatgc gcccgatcgt gaccgtgccc atcagggaat
     4921 cggtgtcatc cccgacgcct ccgcccatac cataataata tgcgccgccc ccgtatcccc
     4981 ctccgggata accgggatac gccgaaggcg cgtgtccgcc gtaatagatg gcccgtttgt
     5041 tgaacccacc gtaatagatc ggtcggcgtg tacccgtgct cacatccccg gcagtggtgt
     5101 cttccctctc gttcatctat cctctcccaa aaaatgattt cgcggctgtt ccgccaaccg
     5161 atcacagatg gcacgcttag cgcgcctttt tgttcgcgtg cttgggcaga aaaccaggca
     5221 acgcggccaa ggccaccgac cacgacaaca acacagcggg cgaccgaaag gggttggcga
     5281 accaactctc gacaaagacg gcacccgtgg cgagcacacc cgacaccacg atcggcgaaa
     5341 tgcgcaacag gaagaatcgg ccttccattc ccacctcgcc gccagcctgc caggcgatac
     5401 gggcccgata acaaagcggc gcgatcaacg caagcgccgc agtcagaaag agggccaacc
     5461 ccaccacccc atattcgcac aggaactgga ggtactcatg ataggcatac gcctgatcgg
     5521 tttgcagcag cgcccactct ttatccgaga gcaacaatcc gacaccgtga aaaaaccctc
     5581 cggccccgca accgaaccag agatgctcct tccacaacgc aagcgcggca tccatacgaa
     5641 cccgtcgtgt ggccatgagc ttctcccaat actccgcaac gggccaggcc tccttcatct
     5701 tcgcggccac cggattgtcc ggcatcagga aacccagcgt caccaccaat gccgccaacg
     5761 cgaacaccgt gaccaaaaac agcagcacct gcgttttttt ggaaacctgc gcatccagat
     5821 acactaacgc atacagaaag agcgccagac ccaagatgcc gtacaggcag accgacaagg
     5881 ggggggaata aaaaagcagg ccgatcagat tggcgagggt cccgaacaag tacaaggtcc
     5941 gccctccccg ctggaaacgc gtcagcgcat cagcgagaag cccccaggac aggagcatcc
     6001 agaacccgaa aaacgtgcct cgcgccagat tcgccgcatc cgccagacac ctctccggcc
     6061 agacacgggc caccgagatc agcgccacac agccgcttgc cacggccatg ccctgcagca
     6121 acatgcgctt cggtgtccgg ctgagcgcgt tccgcacggc gagccccacc accacacagg
     6181 cgaccaggac acagaattgg gagaaccccg ccacagcctc cacgcaaaac ggcgcccagg
     6241 caatcggcgg agtgctgatc cgccacacat cggcatccgg aaggtacacg agcgggcatc
     6301 cgccgttcag ccactggatc atcagcatgc cgaccagcgc ggcaccgata tacagcagcg
     6361 gatcccagta cagaccgcgc accatgcgct gacgggcatc cgccagcgtc tcaccccgat
     6421 acacagaggg caacaggatc atcagttcca cgaccgccag ggaaagccac agaaacggga
     6481 tgaacggaag ccaacgcgaa ctcccaaatc cggcaaaagg gaaaaaacaa acgagggcca
     6541 catggatcgc gagcccgtat tttgtcagga tcttctgcat catacacgga acatcacagg
     6601 caccgcataa gaaatccgcc tacgcatcgc atgcggtgaa ggcggattcg gcgtgcgcat
     6661 gattctcgtt cagcccatcg atcagtaccg gaactgcaat cccagcagcg cccggtactg
     6721 gtcataatca tacgcacggt tgtccgacga acggtcttca aattccagcc caccgtacac
     6781 cgacacatac cgcatcaact ggtaatcggc gcgcgcgcgg atcgagaaga tatcatcggt
     6841 ggcggccccc atattcctgg ccaccgcgtt ttggtactca tcctcctcac gccggtaggc
     6901 cacgtcgaga cgcgtcgtga gcttacgcat cggccggtag gtgacgcccg ccgaaatcgc
     6961 gtgcacctgc atcgcctgat ttacctgcgt ctcgctgggc tggtaataac tcgaaccggc
     7021 caccgtggcc gcccacttct tgctgatcag ccaactggcg tccaaactgt acttccatcc
     7081 agtcaagagg tcgccttccg cataatcaaa ccactccaca ccggtcagag cccggtacga
     7141 gatccgctcc gtggcccgcg aaccgaaacc agccatcagg gagtatcccg aactgctgct
     7201 gtcaacgccg cctacggccc cgttactctt atactgctga tacgtgccgg ccagcagcac
     7261 gttgctcttt tccgaaagct tgcgggcgaa ttccagcccg gtgctccact cgcgccagcc
     7321 gtagagcgga gcgtatctac cctggtcgtt ccggtaattc agatcggaaa acatgccgct
     7381 gagcgtcacc ccggtcttct cgctgatctg gtaggacaac gcgctgttga tctcatgcag
     7441 gtcccggtcc cgccacaacc ccctgccgcc atcgaggatc gaatcattct cccggctccg
     7501 gataaaactc tgccccagca caaaacgcca tcctttggcg gattcgcgat agaacgacac
     7561 ccgttccccg taccggttct tatccagttc atcatagtcg cggtaataat cacgaccgaa
     7621 ccagagcccg gccgacaacc cccagtcatt accggtatac gtcagatcca ccccggggtt
     7681 caccgaaaaa atctcatcgc tgacctcggt tcggtccgag tccacattgg aatcgtacat
     7741 gtactccaag ttcacgtacg gcgagaccgt taggttgttg aacttgaatc cgcgttcctg
     7801 ggctcgggtc accccgcaca ccagaatcag cgcgaacatc aggatgatac gcgcccgtgc
     7861 tacaaaaacc gttcgtgtta ccataacccg tactccttgc attaccgcga tctcaaaggg
     7921 ggacatcgtg ttcgtcaggg acatccgcag aacgttttaa ccctatacac ccttcttttc
     7981 ccacgctcct gtaagaaatc gtccccgatg atcgtcaact tctggttttg gtatccgctc
     8041 ggataaaacg ggttccgcgg cacacggttg atcccgtaat ccagattgct gcccgaaacg
     8101 gcggtcgatg aagatgcgct ggccccgccc aaacgagctt tttccaacga aagggacgcg
     8161 cctttgccgt ccgcgggaag gctggcgagc atatcggcca acaggcggtc cagcgaggca
     8221 tgtccgatca gccgcaggat gtcctcgtgc cgcaacggca aagcctcggc atccgccgcg
     8281 gccagcatcg catcgtattt gccctcgttc aacacctgcg ggatccacgt ctgcgccaga
     8341 ccgcgcatgc ggtcatccgg caacacggca agcagatcac gctgcagatt cgggcaatcg
     8401 gcacctttca agaacgccag gaccgtaaac gtgttgcgga ccgaggcctc atccgtctcg
     8461 gcattcctta cggtcacggc tcccagcacg ttggtcgcga tcatgccgta ctgctcgcag
     8521 gtgagctggt tgtattcgcg gttaaagcgc ttggccaact cgtccgtcac cagcggcaga
     8581 tattccacgg gcacccccgc gaaaaccgcc gcgatcacat cccgcttggt ctccgcgtcc
     8641 gccccggcga tacaagccac cgccgaacgc acaaacgccg cccccttctc atccgggttg
     8701 gcgggcagac gcgagacggc cttgagcagg cgctgcgtga actccacccg gtccgaggga
     8761 gcgacctgct ccagcgtgtc gcgcaacacc gccgcatccg tcaccgacgc gccgatcttc
     8821 ttgagccact gcgaacgcga aaggaattcg ctctgctgcg ccaaccccac aacagcggcc
     8881 caggccaagc ccacgcccag cgcgcatcct aaaactcttt ttgacttttt catctctctc
     8941 agcctccctc cctctcttcg cggctcaata ccacgactcc ggaacccaaa ccacatcacc
     9001 gggtttgagg gcgatatcca aatccgagcg gccctctttc ccgatcttgt caatgtccac
     9061 cataaaccgt ttcaacgagc cgtccttctc ccgccgcgtc acccgcacat gccgcttgtc
     9121 cgccagagcc gtggccccgc cagccaacat cagggcccgc gtcaccgtca gatcccgcgt
     9181 cgagggcatg ttgacgggac ccggccggac gatctcaccc atcaacagca ccgaacccca
     9241 cggcgatttc atgcccgtgt tctctgtata cacgaaaccc accgtaacct gaggctcgat
     9301 gtaataatcc ttgtaggctg ttttgatctt ctcctgaagc tccataatcg taaggccttc
     9361 gcagcggatc gcgcccacca gcggcatgag gatttcgccg ttcgcgttca cttccttcag
     9421 cccctcctgc acggcctccg aaccggaggc cgtcaccgcg acacgcaacg tcagcccggg
     9481 tttgatcttc ggctctccct cctccacata ctgcgcctgc tcatcaaacc ccatcgcgtc
     9541 ctcggcagcc gtccggcacc cgttcacgga aaggacagcc cagcacagta acccgaagcc
     9601 gaggcacttc aacatcacgc tcttcaagcc aaactctctc atctataagg gcgtttcaca
     9661 cgacaccctt ggttagccat tgcccacaga gtaccatttg ccgcagaagg cgacaagggc
     9721 cgaaaagcta aaatcggttt tattttccct tccattttat agcccttcca acctccgtaa
     9781 cgcgcaaatt gtctatgttc cgccataacg ggccggttat ttcgtaaaat cacgcaggtg
     9841 gccgacgagc gaaagcagcc cgcgccagta gaatttcacg tcccccacgg gatcccgggt
     9901 ggacaacaga cggcgcacca ccgtctccgg gtgcaggaac gcctgataca cctcgcggac
     9961 cgcggctttg atccgcgctt ccggaacccc cgagcgcatc acctgccgcc gcatgtcata
    10021 atcatcccat tcgcgcgtca acagaagccc ggcctgatcc aactcccgga agagcggcgt
    10081 gcccggatac ggcaccacca tcgtcacttg cagcgtgctg gcatgccccc tgcgcaacag
    10141 ggaccgcgcc aaccggacgg tgttggcgat ttccccctcg ccctcccacg ggtagccgaa
    10201 catcaccgtg acgtgcacat ccagaccggc gcgcgcggcc cacttcacgc cctcttcgat
    10261 ctgcgccacc gtgagacctt tttgaagacg gtcgagcgtg gcctggttgg cggactccaa
    10321 tccgaaaagc accagccgga atccggcctt gcgcatgagc cggtaggtct cttcgtccag
    10381 acatccgaac cgcatattgc agtcgatgcg gattttccgg ttataaccgc gcgcgatcat
    10441 ctccccgcag aagcgcttca accacgcccc ggcaggcagg ctgccggtgt catccatgat
    10501 ctcccggatc ccgtaccggc tgatcagatc tccgatctcg tccaccacct caaccggatc
    10561 acgcacacgg taggtcggat aaagcgtggt ccacgagcag aaggtacact tgccgtgcca
    10621 acagtcacgg cccgacatga tgtaggttcc gggcgttttg cggaaattgc cgttgcgctc
    10681 ggcatagaga ggccaacagg tcagctcgcg gtcgatccag ggcagggtct tgagatcgtg
    10741 atcgagccgg aaggggccgc tgtcccgcac cataccgtcc gcatcacgcc agtagacgcc
    10801 gggctccaga cgcgccgtgt ccggcaggcc gcccccagcc gcgcgcagga cctccagcag
    10861 attgaccagc agaaaatcgt agtcgccccc ggtcagcacg taatccaccg gacaggcttc
    10921 gaaactttcg cgcggcaagg ccgtgacatg atctccgacc agcaccaccc gcaccccggg
    10981 gtgccgcgcc ttgagttccc ggatataggc ccaatgacgc cgcaccaccg gcgtcttggt
    11041 ctccagcatc accacctccg gggcgaatgc gtccagccgc gcgtcaaact cctcgggcgt
    11101 ccactccgaa gcgatgccgt ccagccaagc cacgtcatgt ccggcggccc gcgcctgcgt
    11161 cgcggcctgc gcgggcacca ccggatagat gtacgtggga cgcgtgaacc actggaactg
    11221 gcggttctgc gaaagcaacg gcaccccttt ttccgagggt agcggcgggt aagcgatggc
    11281 gagacgcatg ggcggattct ctctctggtt cgtcacggaa cacgcgttcc ggattcccgg
    11341 tcggtcttcc ggcgggtaaa gaacatatta aaacacagtg gccggaaagg ggacactcaa
    11401 aaatggcggc attgttttcc gccccgattt ttgtcatgat accaatccat gcagacacgc
    11461 ccctcagagg aactcaaccg ccggaacgcc tcgttcgaca atcgcctgcg ccccactggc
    11521 ttcgccgagt ttgtgggcca acagaaggtg cgggagcgat tgatgctggc ggtggaggct
    11581 gcccgccagc gcggggaggc gcttgaccat gtgctgcttt cgggtccgcc cggactcgga
    11641 aaaaccaccc tcgccttcat tctcggcgac gccatgggcg tgcatgtcaa aaccacatcg
    11701 ggcccggtca tcgacaagcc cggcgatctg gccggactgc tcaccagtct ggaacccggc
    11761 gacatcgttt tcatcgacga aatccaccgc atgcagcgga ccgtggagga gtatctgtac
    11821 tccgcgatgg aggatttcgt catcgacatc ctgatcgacc aagggcccaa cgcccgatca
    11881 gtccggctgg aacttccccg tttcacgctg gtgggcgcga ccacccgcag cggcctgatc
    11941 tccgccccgc tccgctcgcg cttcggcatc accaaccgcc ttgattacta ttgcattgag
    12001 gaactcagcg acatcctcac ccgttccgcc gtccggctcg aggtcagcat cgatgagggc
    12061 ggcacgcggg aaatcgccgc gcgggcgcgc ggaaccccgc gtatcgccaa caacctgttg
    12121 cggcgggtac gcgattatgc ccaagtgcgc gccgggaacc acatcaccca agaggtggcg
    12181 ggcgaggcgc tcgcgttgct ggagatcgac ccgcaagggc tggatgagat ggatatccgg
    12241 atcctcgaaa cgctggccgt gaaattcaat ggcggcccgg tggggctcaa caccatcgcc
    12301 gtttccgtgg gcgaggagcc cgacaccatt gaggaggtct acgagccgtt cctgattcag
    12361 gagggctatc tggaacggac cccaaagggc cgtgtcgcca cccctgcctg ttaccgccgc
    12421 ctgggtctca cccccgccgg ccgtcagtgc gagctgccgc tataggacta atgcgggcaa
    12481 agcccgcaac ctcaaaggct gtgagactgt gaggctgtgg ttaaatgatc gatcccgatc
    12541 ccgatagcga tcccgatccc gatgaaaccc tgaaaccaaa gagagttgaa ggttcaactg
    12601 ctctttttag gataaattga caggattaca ggattaacat gattttttag gataaattga
    12661 caggattaca ggattaacat gattttttag gataaattga caggattaca ggattaacat
    12721 gattttttaa ccctgaaaat cctgtcatcc tgtcaattta taggcaagtt aattcgtgcc
    12781 ttagtttttc catatgtgaa tcttcctgtt acgaacctct cccgaacagc ccgctgcgcc
    12841 actcgccgaa caacaggctg tcaatctcaa ccagcccctg cgcctcgtaa gcggcgcata
    12901 ccgcgtcata ctgcgtgtca agaatgcctg acaacaccag ccgtgcgcgc gggtccgcgc
    12961 tcatggaccc cgccacctgc ggcgcgaacc ggatcagaac cggcgcaaga atgttcgcca
    13021 ccacgatatc ggctggcggg tgcgcttgcg aaaggtcgtc cagcgtgaag gggatcgaca
    13081 cgccgttcag cgccgcgttc tcctctgaca cgcgcatgca gtcgggatca ttgtcgaagc
    13141 cgcgcacatc cgtgaaaccc agcagcttcg cgccgatcgc caaaatgccg gagccgcacc
    13201 ccatatcgag tacggagcgc tgaatgtcct ccgccgccaa ctggtccaaa aagcggaggc
    13261 aggcctgcgt ggtggcgtgt ttgccggtgc cgaagctcaa gccgggatcg agcgtgatca
    13321 cgcactcgcc gggttgcgcc gtgtacggtt cccacgaggg gcgcaccacc acgcggggag
    13381 aaatcttctc aacgtggaaa aagcgtttcc agctttcggc ccagtcggtc tgcgcgacgg
    13441 ccgtgaccgt cggctcgacc gtcacgccga gcagtgcggc ggcggcaagc agcgcggcct
    13501 gcgtgtgcgc cacaccgtcc ggttcatccg gaaagagcga gacacggcag acgccgcttt
    13561 ccacatcgta ccacgaggtc ggcgtaaagc catcgtcgcc gatgatctca aacagcgcgt
    13621 ccgccgcctc gggcggcagc gtggcggtga tgcagggaac ggaagcggca gagtcggtca
    13681 tggcgtcaca tcacaccacc gtgcagaggg tgtagtgggc gttgacttcg gtcttgatcg
    13741 cttcg
//
LOCUS       DAOSRB010000062        13661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4580_length_13661_cov_7.643613, whole genome shotgun sequence.
ACCESSION   DAOSRB010000062 DAOSRB010000000
VERSION     DAOSRB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13661
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4580_length_13661_cov_7.643613"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLZ74_04940"
     CDS             complement(<1..592)
                     /locus_tag="PLZ74_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOR97701.1"
                     /translation="MDDNFLQFASYTICNRAIPTVEDGLKPVQRRIMHSLWEKDDGRF
                     IKVANVVGHTMQYHPHGDASIADAIVNLTNKRYLIEGQGNYGNIFTGDPAAASRYIEC
                     RLTELARNELFNPKLTSFIPSYDGRNKEPVLLPCKLPLLLMLGAEGIAVGLSTSILPH
                     NFIELLEAQVAIIQKKPFQVFPDFQTGGIMDVSEYAD"
     gene            complement(796..2622)
                     /locus_tag="PLZ74_04945"
     CDS             complement(796..2622)
                     /locus_tag="PLZ74_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011174900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOR97702.1"
                     /translation="MSASTYDDKHILTLSAIEHIRQRTGMYIGRTGDGSQYEDGIYIL
                     LKEVIDNAVDEFIMGYGKRIEISLEYDTGEVVVRDYGRGIPLGKVVECVSEINTGAKF
                     NDDVFQLSVGMNGVGTKAVNALSTQFEVRAFRDGEYSEAFFERGDLVSQKRGKCASPQ
                     RNGTYVRFTPDADIFKKFQFREEHVLRRLRMYAYLNAGLTLELNGTKIVSENGLLDLI
                     HDEVQFEKLYQPFHYRSKMLELVFTHTNRFSEDYYSFVNGQFTNDGGTHLSAFKEGLL
                     KAVNEFSKKRFDGDDVREGIVGAIAIRLKEPIFESQTKNKLGNQEIRSELVAEVKRVV
                     EEMLHRDPDEAAKLISKVEETGKLRAELNAVKKLVRERSRAVTVRVPQLKDCKRHLHK
                     KKGTGLDTMIFITEGQSAAGSIVSCRDVNTQAIFTLKGKPLNVCDSKRDALYRNVEIY
                     NLMQSLDIEETVDRLRYQKVILATDADVDGLHIRNLMITLFLRFFDRVIKEGHLYILE
                     TPLFRVRNKKKTLYCYSEEERVAAIEACGRNPEITRFKGLGEISPDEFKAFIGKSMRL
                     TPVDSSRDRDLKDTVDFYMGRNTQERRQYIMDNLVITEESLS"
     gene            complement(2733..3248)
                     /locus_tag="PLZ74_04950"
     CDS             complement(2733..3248)
                     /locus_tag="PLZ74_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR97703.1"
                     /translation="METSLGAITAELYPDKAPVTVSNMLAYIDAKHYDATIFHRVIDG
                     FMIQGGGFTRDMVQKKTRAPIRNEADNGLRNERGTLAMARTRIVDSATSQFFINLVDN
                     GAHLDFKEKSDPGWGYCVFGRVIEGMEVVDRIAKVRVGSSGHHQNVPLDPVVMISVRR
                     KDASPASATPK"
     gene            complement(3467..4195)
                     /locus_tag="PLZ74_04955"
     CDS             complement(3467..4195)
                     /locus_tag="PLZ74_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOR97704.1"
                     /translation="MDRKYAILGDIHANLEALTAVVGDARQKGVTDFVCVGDVVGYNA
                     SPSECIRMIRELGCVTVRGNHDHYCSYDESLDDFQPLAASVIAWTRRQLSEEDGKWLR
                     ELPYQKACGGFTLVHSTLDMPERWGYVFDTLAAEANFSYQMTALCFHGHTHVPMIYEK
                     KREMMRVEPCKVKVAFGQKFFVNVGSVGQPRDADPRSSYVLYCAKTKEFEFVRVAYDI
                     ETAMEKNLKAGLPERLALRLAQGR"
     gene            complement(4209..4889)
                     /gene="ispD"
                     /locus_tag="PLZ74_04960"
     CDS             complement(4209..4889)
                     /gene="ispD"
                     /locus_tag="PLZ74_04960"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOR97705.1"
                     /translation="MNTAIIVAAGKSTRMGSNTDKAFLSLGAKPVLAWSLLAFEQCTD
                     IDQIVLVVRKDQVLAAKSVAQMFGISKIRTIVAGGSRRQDSVQNGMREMDPDTRMVVV
                     HDGARPCVTPQLISDTIKCAKRNGCGVAAARIWDTIKYVERGSTVDHTVDRAKLWAVQ
                     TPQAFTTALLQRAYQAVEEQKVTVTDEASAVELLGEPVRLVEWRYPNIKITTAEDLPL
                     AAAAMKIM"
     gene            complement(5144..6289)
                     /locus_tag="PLZ74_04965"
     CDS             complement(5144..6289)
                     /locus_tag="PLZ74_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOR97706.1"
                     /translation="MKVKNIKWRNVFETIRSEILLGKFDVRERFPSEVQLCRRFGVSR
                     PTVRLALDELKRTGILEARPGSGTYLSDMARNLTGHLGLIVPGIASGEIFPPICAEIA
                     RYAREAGYTLIFGDASSKDPKVRARQAIDLARQYADERVAGVFLEPIELVPGAAETTE
                     KILTILSDHNMPVVLLDRDVVPPPDRSGYDLVGIDNVQAGYRLARHLIDCGARRICFF
                     MRVGSAPTVSLRIQGARGAVLDAGLPWASASVCVAEIGDAEPIKKLVGGRCLPDAFIC
                     GNDATAAALLQTLNSLGKQVPRDFLLAGFDDVKIATLTSPPLTTIRQPCDLIARAAVD
                     TLLTRLRDPSLPPREVLLDVSLVVRHSTVSRVCADQRFRAGRNPIPL"
     gene            6402..7361
                     /locus_tag="PLZ74_04970"
     CDS             6402..7361
                     /locus_tag="PLZ74_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOR97707.1"
                     /translation="MFIGVDMGTTSFAAVLVNEKGEVLRTLTHPNDCTNEGLPPDVHE
                     QDPDAMADMAEELVAELEAAAGGSVRRIGWTGQMHGVVGVDENLKAVTPFVTWRDARR
                     YGGHVMDGWARAGRKLFRCLPLPGYVIARRTGRCVIDETFLHSWHLEKAGEVPQEWLP
                     DLVPGSMIGDNQAGVFAVQHILPGYAVVNLGTSGQLSIVGEGHGGERRPFPGGRVLRC
                     RASLVGGRAFAELKKELGLSWDEMNARAQSDPRIAACVRSIADDLAGDLDLTGVTGLA
                     GIGNALVRNPCLKTAVAERFKMPCVVPPVAEIAAWGAALEVMT"
     gene            7358..8575
                     /locus_tag="PLZ74_04975"
     CDS             7358..8575
                     /locus_tag="PLZ74_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR97708.1"
                     /translation="MKSPAKLWFLAITAALSGFLFGFDTIVISGAEQQIQDLWNLSPA
                     LHGWVLSAALWGTVAGSLFGGSVIDLMGRKKSLFCVGVLYFVSALWSAFPSGPYTLMI
                     ARFIGGLGVGVSTIAAPVYIAEISPAESRGRFTALFQFNIVFGVLVAYASNYFFKDYG
                     ESAWRWMLGVEAIPAAVFTVLCLFLVESPRWLKMRESHQAPIRPDFRSFFTRENRGPI
                     LLAFFVAFFNQLSGCNAVNYFAPRIFQMAGLGRDSAFLSSFGLGTLNFIATFTGLWLI
                     DRLGRKTLLLIGGIGYVVSLSAASLFFGIGNGPLAAASIFLFMASHAVGQGTVIWVLI
                     SESFPDRFRALGQSFGSFTHWMFAALITLFFPMAAAAFAPWKIFAFFGVMMVFHLFWV
                     IFVVRETKGKALA"
     gene            8844..10151
                     /locus_tag="PLZ74_04980"
     CDS             8844..10151
                     /locus_tag="PLZ74_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOR97709.1"
                     /translation="MRGITKTVAAVAAVATLGAGAARAETGFNAEVSGVTLTQRENTR
                     IVDIGYTLTGEPAIITLAIETNGVAIPDSAVTRLSGAVSTVIQPGTYTIIWNAGADWP
                     EHEVTNARARVTAWSTNAPPLYCAVDVAGGPAADNYPVYYYTSAEAVPGGVTNDLYKT
                     FRILMRKIPPTGGEGFMMGSPSNETGRDAMREDCHNVKLTKAFYAGVYEITQSQWYQV
                     MNSWPSKWSNNDYKLTRPVDQVSWNDIRGGVWPGVPRGSGLPGAESFVAKLRARAAMP
                     GLDLPTDAQWEYACRAGTSGALNDGTVNITNTDSDARMDLVGRYQYNGGKVLNNGTWS
                     DPPRDCTTANATATVGSYAPNTWGLYDMHGNLWEWCLDWLVDHLGTDSVEDPIGPDSG
                     SVRVIRGGSWGSTATGCRSAYRRWNDPSTEFSGYGFRLVRTLP"
     gene            10301..10540
                     /locus_tag="PLZ74_04985"
     CDS             10301..10540
                     /locus_tag="PLZ74_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97710.1"
                     /translation="MRQMTRMAAFVAMVLLAVRIGAMDPVSEVSAAAPLDTRSIFVER
                     AAEDQTLNSRSFDVDWSEMRKLNTKKIVGTVMLMR"
     gene            10648..13335
                     /locus_tag="PLZ74_04990"
     CDS             10648..13335
                     /locus_tag="PLZ74_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOR97711.1"
                     /translation="MNRVFAVALVGMAVGTTGAGTLQLAPPFTKGMVLQRDRTVPVWG
                     RTAPGAAVSVEFGGETVRTVGGKDGAWRVDLPPQAASKTPRKLTVKADGETRVVEDVL
                     AGEVWLCSGQSNMHMPIALRQPRYGDGHGAMVAQVTRKPFIRYAEAPCGGGWHALTPD
                     FLRSGWKCALPVHYALELYAQIDVPIGIVVAASGGSNIDSWNPANGPKAGLYHAAIAR
                     LAPFGLRGVIWYQGESNVHESWLYTRKLHELYDGWSKAFGNPNLRFDLVQIAPYAYGA
                     DMNGLKDREFARFQEAQMKFADEEPNASVTVINDLGDLRDIHPNNKILVAKRLALHAL
                     KRDYGFSDIEADSPTPAEATVVSNRVRIVFDHAKDLYVYNAERTLEAAFELCGADGVW
                     TKAQIVNFAKTGWAQFGNIDGNAVELEAPGVLQPIKVRHAFTSPWKGCLYNQVNLPCS
                     TFLRSLPFTRMNETYRPQFHFTTPSGWINDPNGLVWKDGVWHLCYQHNREKADWGPMM
                     FWGHAVSTDLVRWLDRGDVISPVNGQACYSGSSAVDHENTAGFGAGAIVSMFTRTGSG
                     SDLAWSTDGETWNLYEKNPALPRLACTHRDPMIVFHKPSGKWVLVIYTEKENVYGVTF
                     FTSPDLKSWKEVADIRGDPRGKKPVGVWGKDRDWLFECPNFVEVPVAGTSERRWVLFD
                     ASCNYAVVDFDGEQFKIAEGPYESLPTREKLYYAGQTFSNAPDGRCVWMAWYHLNIPH
                     PTLFTQLMSVPCDLALMQTPGGLRLRRTPSKELETLRMGSAKPLEEIEGDVLEVSLDC
                     VVVSGAKAAISLRGVDLVYDGASKTLACAGETIDWPLQDGRFRARVFIDRAGFEIFSE
                     DGLITFPRRVVCNPKDRKLVASPATGVTDVRHRAWCLVR"
     gene            13415..>13661
                     /locus_tag="PLZ74_04995"
     CDS             13415..>13661
                     /locus_tag="PLZ74_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97712.1"
                     /translation="MTVIVAGLGFVGAVCAAETPAVHLIPNALETRPITTTADVILAK
                     GFTVTLEVDFDGVGDDETLYEAGAVKLAFRLAGKTKGL"
BASE COUNT         2724 a         3975 c         4126 g         2836 t
ORIGIN      
        1 cgtccgcata ttccgagaca tccatgatgc cgccggtttg gaaatccggg aagacttgga
       61 acggtttttt ctggatgatc gcgacttggg cctcgagcag ctcaatgaag ttgtgcggca
      121 ggatcgaggt ggagagcccc actgcaatgc cctccgcgcc gagcatcagc agcaacggca
      181 gtttgcaggg cagcagcacc ggctccttgt tccggccgtc gtagctggga ataaaggagg
      241 tcagtttggg gttgaagagt tcgttgcgcg caagctctgt gagacggcac tcaatgtagc
      301 gcgaggcagc ggcaggatca ccggtgaaga tgttgccgta gtttccttgg ccttcgatga
      361 ggtagcgctt gttcgtcagg ttgacgattg cgtccgcgat cgacgcatcc ccgtgcggat
      421 ggtactgcat cgtatggccg accacgttcg cgaccttgat gaagcgtccg tcgtccttct
      481 cccacaggct gtgcatgatg cggcgctgca cgggcttgag tccgtcttcg accgtaggga
      541 tggcgcggtt gcagatggtg tacgaggcga actgcaggaa gttgtcgtcc atcaaatcgc
      601 gcagcgggcc ttcgccaccg tgcgtgccag tcgcagacga cgaggagccg ccatcttgtt
      661 cggagtcgtc cgcctcctct tcggcggatg cggtcggcat ggcgtcgaaa agatccatgt
      721 cgtcaaacag gctgggcggc tggatggcgt ccgcgtccgc cttttttttg cgtttcggct
      781 ttttgggaga cttgctcacg acagcgattc ctcggtgatc accagattgt ccatgatgta
      841 ctggcgccgc tcctgagtgt tgcgccccat gtaaaaatcg accgtatctt taaggtcgcg
      901 gtcacgtgaa ctgtcaaccg gtgtcagccg catgctcttg ccgatgaacg ccttgaactc
      961 gtccggcgag atttcgccca ggcctttgaa acgggtgatc tctggattcc gcccgcaggc
     1021 ctcgatcgct gcaacgcgct cctcttcgct gtaacagtaa agggtcttct tcttgttacg
     1081 cacgcggaag aggggggttt ccagaatgta gaggtgacct tctttgatga cccggtcaaa
     1141 gaagcgcaaa aagagggtga tcatcagatt acggatgtgc aaaccatcaa catcggcgtc
     1201 ggtggccagg atcactttct ggtagcgcag acggtcgacg gtctcttcga tgtcgagact
     1261 ctgcatcaga ttatagattt cgacgtttct gtacagggcg tcgcgctttg agtcgcacac
     1321 attgagcggc ttccccttga gcgtgaagat cgcctgtgtg ttgacgtcgc ggcaactcac
     1381 gatcgatccc gcagccgact ggccttcggt gatgaagatc atggtgtcga gcccagtgcc
     1441 ctttttcttg tgcagatggc gcttgcagtc tttgagctgc ggcacacgga ccgtgaccgc
     1501 gcgcgagcgc tcacgcacca gcttcttgac cgcgttgagt tcggcgcgca gcttgccggt
     1561 ctcttcgacc ttgctgatga gcttggcggc ctcatccggg tcgcggtgca gcatctcctc
     1621 gaccacacgc ttaacctcgg ccaccagctc ggagcggatc tcctgattgc ccagcttgtt
     1681 cttggtctgc gactcgaaga tcggctcttt gagccggatc gcgatcgcac cgacgatgcc
     1741 ttcgcgcacg tcgtcgccgt cgaaccgctt tttgctgaac tcgttgaccg ccttgagcag
     1801 gccctcttta aaggccgaca agtgcgtgcc gccatcattg gtgaattggc cgttgacgaa
     1861 cgagtagtag tcttcactga atcggttcgt atgtgtgaag accaactcca gcatcttaga
     1921 acggtagtgg aagggctgat acagcttctc aaactgcact tcatcgtgga tcagatccag
     1981 cagaccgttt tcggaaacga tcttggtgcc gttcagttcc agagtcagcc cggcattgag
     2041 ataggcgtac atgcgcaggc ggcgcagcac atgttcctcg cggaactgga actttttgaa
     2101 gatgtcggca tcgggggtga agcgcacata ggtaccgttg cgctgtggcg aggcacactt
     2161 gccgcgcttc tgcgaaacaa gatcgccgcg ctcgaaaaag gcctccgagt attcgccgtc
     2221 gcggaaggca cgcacctcga actgtgtgga gagggcgttc accgccttgg tgccgacacc
     2281 gttcatgccc acagaaagct ggaagacatc atcgttgaac ttggcgccgg tgttgatctc
     2341 cgagacgcac tccacaacct tgcccagcgg aatgccgcga ccgtagtcgc gcacaacgac
     2401 ctcgccggtg tcgtattcca gagagatttc gatccgcttg ccgtagccca tgatgaattc
     2461 gtctacagcg ttgtcgatga cctcctttag aaggatgtag atcccgtctt cgtattggct
     2521 gccgtcgccg gtccggccga tatacatgcc ggtgcgctgg cggatgtgct caatagccga
     2581 aagcgtcagg atgtgcttat cgtcgtacgt gctggctgac atggaaaact gaaaaaacgg
     2641 tgcgtgaaaa aacaacccgg acatccgcgc cgaaccggtc cgccatcgtc cacatggaat
     2701 gtttacggcg ccgccgcgat cccgaaccgc tcttatttcg gagttgcgga cgcgggtgac
     2761 gcatctttgc gacggacgga gatcatcaca accggatcga gcggcacgtt ctggtggtgg
     2821 ccggacgacc cgacccgcac cttggcgatc cggtcgacca cctccatccc ttctatcacc
     2881 cgcccgaaga cgcaataccc ccaccccgga tcactcttct ccttaaaatc gagatgggcg
     2941 ccgttgtcaa caaggttgat gaaaaactga ctggtcgcgc tgtcgacgat ccgggtccgg
     3001 gccatggcca gcgtaccgcg ctcgttccgc agtccgttat ccgcctcgtt ccggatcggt
     3061 gcacgggtct tcttctgcac catatcacgc gtgaacccgc caccctgaat catgaaaccg
     3121 tcaatcaccc ggtgaaagat ggttgcgtcg taatgcttgg catcgatgta cgcgagcatg
     3181 ttgctgaccg tcaccggcgc cttgtcggga tacaactcgg ccgtgatcgc gcctaaactg
     3241 gtttccatca caatttgcac gttcttggac tccttagata ccgttgcggg agtggccgta
     3301 tttccatagg ccagatccgc aaacccgatc cctgcgacaa caagcaagac aatagaaaaa
     3361 cccttcatac cctacatccc ccaaaaaaaa ttcgaggaga aagcgtaacg cgacacgcgg
     3421 gaaaaagcca gcctcttttc aagtccgcgc agtccggaac cgacggctac cggccctggg
     3481 ccagacgcag cgccaaccgt tcaggcagac ccgccttcag attcttttcc atcgcggttt
     3541 cgatgtcata cgccacccgg acgaactcaa attccttcgt tttggcgcaa tacaacacat
     3601 acgaactgcg cggatccgca tcccgcggct gccccacgct acccacgttg acaaaaaatt
     3661 tctggccgaa cgccaccttc accttacagg gctcgacccg catcatctcg cgttttttct
     3721 cgtaaatcat cggcacatgg gtgtgtccgt ggaaacagag cgcggtcatc tgataactaa
     3781 agttcgcctc tgccgccagc gtatcgaaaa cgtagcccca ccgttccggc atgtccaagg
     3841 tgctgtgtac caacgtgaac ccgccgcacg ccttctgata gggcagttcc cgcagccact
     3901 tcccgtcctc ttccgagagc tgccggcgtg tccaggcgat cacgctcgcg gccagaggtt
     3961 gaaaatcatc aaggctctca tcgtacgaac aatagtggtc gtggttgccc cggactgtaa
     4021 cgcaaccgag ttcgcggatc atccggatac actccgacgg gctggcgttg taccccacca
     4081 cgtctccgac acacacgaag tcggtgaccc ctttctgcct ggcatccccc accaccgcag
     4141 tcagtgcctc cagattcgcg tggatgtcgc ccaatatagc gtattttctg tccatgcgct
     4201 cgcttcgctc acataatctt catcgcagcc gcggccagcg gcaaatcttc cgccgtggtg
     4261 atcttgatgt tcggatagcg ccattcaacc agccggacgg gctcgcccag caactccacg
     4321 gcactggcct cgtccgtcac cgtcaccttc tgttcctcta ccgcctgata ggcccgctgc
     4381 aacagggctg tcgtgaacgc ctgcggcgtc tgtaccgccc acaacttggc gcgatcgacc
     4441 gtgtgatcca ccgttgaacc acgctcgaca tacttgatcg tgtcccagat ccgcgcggcc
     4501 gcgaccccgc aaccgttgcg cttggcgcac ttgatggtat cggagatcaa ctgaggcgtg
     4561 acacagggcc gcgcgccgtc atgcacgacc accatgcggg tatcaggatc catctcccgc
     4621 atgccgttct gcacggaatc ctgacgccga ctacccccgg ccacaattgt ccggatcttg
     4681 gaaatgccga acatctgcgc cacgctcttc gcggccagca cctgatcctt ccgcacaacc
     4741 aacacaatct ggtcgatatc ggtacattgc tcaaaggcca gcaacgacca ggccaacacc
     4801 ggcttcgccc ccagactcag gaatgcctta tcggtattgg agcccatccg cgtacttttc
     4861 cccgcagcca ctatgatcgc cgtattcata ttctccttaa acgcatccca tcctcattcg
     4921 atccgtccat gcgacatcgc tcgtccacct gacgcgtctc gacacccaaa caccatgccg
     4981 caatccatgc gatgatgata tcagcagggg caacagacgt caagcggacc agctcaatcg
     5041 ctagagcgta aatccccgtt tcggcttccg acgcgacgga gcggtcatgg tgatgaccgc
     5101 agccgtggca gccatgcagc cgggatacgg caacaccgca cctttacaac gggatcgggt
     5161 tgcgtcctgc ccgaaaacgt tgatccgcgc acactctgct tacggttgag tggcgaacga
     5221 cgagggaaac gtcgagaagg acttcccgcg gcggaagcga ggggtctctc aggcgcgtaa
     5281 gcagggtatc caccgccgcg cgcgcgatga gatcgcaagg ctgccggatc gtggtaagcg
     5341 gcggtgacgt gagcgtggcg attttgacat catcgaaacc agccagcaga aaatcacgag
     5401 gaacctgttt accgagggag ttgagcgtct gaagaagggc agccgctgtc gcgtcgttcc
     5461 cgcagatgaa ggcatccggc aggcaacgcc cgccaacgag cttcttgatg ggctccgcat
     5521 ccccgatctc cgctacgcac acactggcgg aagcccaagg gagtccggca tcgaggacgg
     5581 caccacgcgc accctgaata cggagcgata ccgttggggc ggaaccgacg cgcatgaaga
     5641 aacagatacg ccgcgcacca cagtcgatga ggtggcgagc aaggcggtaa ccggcttgaa
     5701 cgttgtctat ccccacaagg tcataaccgc tgcgatccgg cggaggcaca acatcgcgat
     5761 ccagcagaac caccggcatg ttgtgatcag aaagaatcgt gagaatcttt tctgtcgttt
     5821 ccgcagcacc cggcacgagt tcaatcggtt caagaaaaac acccgccact cgctcatccg
     5881 catattggcg tgcaaggtcg atcgcttgac gcgcgcgcac tttgggatct tttgaagaag
     5941 cgtcgccgaa aataagcgta tagcccgcct cacgggcata gcgcgcaatt tccgcgcaga
     6001 tcggcgggaa aatttctccg gaagcgattc ccggaacaat caggccaagg tgcccggtta
     6061 agttacgcgc catatcgctc aggtaagtac ccgaaccggg gcgcgcttcg agaatgcccg
     6121 tgcgcttcag ttcatcaagc gcaagccgga ccgtcggccg tgaaacgcca aaacggcggc
     6181 agagctgcac ttcactcgga aaacgttccc ggacatcgaa tttaccgagc agaatctcag
     6241 agcgtatcgt ctcgaaaacg tttcgccact ttatgttttt gactttcacc gcgcgtattg
     6301 taccacgttc cctaatgttt gttaattgac attacagctt tttgaaagct gtaatgactt
     6361 ggcccttgca tcagtaaacg aactttgcgg ataatggggt catgttcatc ggggtagaca
     6421 tggggaccac atcgttcgcc gctgtgctcg tgaatgagaa aggtgaggtg ttaaggacgc
     6481 tcacgcaccc gaacgactgc accaacgaag gcctgccgcc ggatgttcac gaacaggatc
     6541 cggacgcgat ggcggacatg gcggaggagt tggtggccga actcgaggcg gcagcgggcg
     6601 gctctgtccg gcgcattggc tggaccggtc agatgcatgg cgtcgtgggc gtggacgaga
     6661 acctcaaggc ggtcacgccg ttcgtcacgt ggcgggatgc ccggcgctac ggcggccatg
     6721 tgatggatgg ctgggcgcgt gcggggcgca agcttttccg atgcctgccg ctgcccggct
     6781 acgtgatcgc gcgtcggacg ggacgttgcg ttatcgacga aacctttctg cattcatggc
     6841 atctggagaa ggcgggagag gtgccgcaag aatggttgcc cgatctggtt cccggttcga
     6901 tgatcggcga caatcaggcc ggcgtgtttg ccgtgcaaca catactgccc ggctacgctg
     6961 tggtgaatct gggaacgagc ggacagcttt ctatcgtggg cgagggacac ggtggcgagc
     7021 ggcgtccttt cccgggcggg cgggtgctcc ggtgccgcgc ctccctcgtg gggggccgcg
     7081 ccttcgccga attgaagaaa gaactcggcc tctcatggga cgaaatgaat gcgcgtgccc
     7141 aatccgatcc gcgcattgcc gcctgtgtgc ggagcatcgc ggatgacctg gcaggcgacc
     7201 ttgatcttac cggcgtgacg ggtctcgcgg ggattggaaa tgcccttgtg cgcaatccct
     7261 gtctgaagac ggccgtggca gaacgtttca aaatgccgtg cgttgtgcct cctgtggccg
     7321 agatcgctgc atggggtgct gcactggagg ttatgacatg aaaagtccgg ccaaactctg
     7381 gtttctggcg attacagcgg ccttaagcgg ctttctcttc ggtttcgaca cgatcgtcat
     7441 ttccggggcg gaacagcaga tccaagacct gtggaacctt tcccccgcgc ttcacggatg
     7501 ggtgctttcg gcggcacttt ggggcaccgt cgcgggttca ctcttcggcg ggagcgtgat
     7561 tgaccttatg ggccgtaaaa agtctctctt ttgcgtgggc gttctctatt tcgtctcggc
     7621 gctctggagc gcgttcccct ccggccccta tacgcttatg atcgcacgtt ttatcggcgg
     7681 tctcggtgtc ggcgtctcca caatcgcagc ccctgtttac atcgccgaaa tctcaccggc
     7741 cgaatcccgc ggacggttca cggccctgtt ccaattcaac atcgttttcg gtgtattggt
     7801 cgcctacgca tccaactatt tcttcaagga ttacggcgag tcggcttggc gctggatgct
     7861 cggtgtggag gcgatccccg cggccgtgtt cacggtgctt tgcctgtttc ttgtggagtc
     7921 gccgcgatgg ctcaagatgc gagagagcca ccaggcgccg atccgtcctg acttccggtc
     7981 ttttttcacc cgggagaacc ggggccccat tttgcttgcg ttcttcgtcg ccttcttcaa
     8041 ccagttgtcc ggctgcaacg cggtcaacta cttcgcgccg cggatcttcc agatggcggg
     8101 acttggccgc gactcggcgt tcctctcctc gttcggtctc ggcacactca acttcatcgc
     8161 cacgttcacg ggactttggc tcatcgaccg tctcggacgg aaaacactcc tgcttatcgg
     8221 cggcattggc tatgtggttt cgctttctgc cgcaagcctg tttttcggca tcgggaacgg
     8281 cccgctcgcc gccgcctcca tcttcctttt catggcctct cacgccgttg gtcagggcac
     8341 ggtcatctgg gtgctcatca gcgaatcctt cccggatcgt ttccgtgcgc ttggacagtc
     8401 tttcggctct ttcacacact ggatgtttgc ggcgctgatc acgctctttt tcccgatggc
     8461 tgcagcggct ttcgcgccgt ggaaaatatt tgcgtttttc ggtgtcatga tggtgttcca
     8521 cctgttctgg gtgatattcg tggtccgcga gacaaaagga aaggcactcg catgatccgc
     8581 cccacagttt gtacttttct tcttgccgtt tttgcgacag cgggttcccg cgccgacgag
     8641 acacgcgcga aggtgttcgc cgacaccgtt ctcgtgaagc cggtgctggc gggagaaatg
     8701 cgtttcagcg gtcaggcgct agatgtacgt acgtcagatc aatctgcgga cttttcgcta
     8761 tacgtcgcac gtcgagtttc ggaaaaatct cgaaaccaac ccaatcggcg gccgaccgtc
     8821 cgccgctata agaggaggaa accatgagag gcataacgaa aaccgttgcg gccgttgcgg
     8881 ccgtggcgac tctgggcgcg ggcgcggccc gtgcggaaac cggattcaac gccgaggtca
     8941 gcggtgtcac cctgacccag cgcgagaata cgcgcatcgt ggacatcggg tacacgctca
     9001 cgggcgagcc cgcgatcatc accctcgcga tcgagaccaa cggcgtggcg atcccggact
     9061 cggcggtgac gcgcctctcc ggcgcggtct cgaccgtgat ccagccaggc acgtacacca
     9121 ttatctggaa cgcgggtgcg gactggcccg agcacgaggt gaccaacgcc cgcgcacggg
     9181 tgaccgcctg gtccacgaac gcgccgccgc tgtattgcgc ggtggatgtg gccggaggtc
     9241 ctgccgcgga caactatccg gtttactact acactagcgc ggaggcggtg ccagggggcg
     9301 tgaccaacga cctgtataag acgttccgta tcctgatgcg caagattccg ccaacaggtg
     9361 gcgaagggtt catgatgggc tcaccctcca acgagaccgg tcgcgacgcc atgcgcgagg
     9421 attgccataa cgtgaagttg accaaggcgt tctatgcggg ggtctacgag atcacgcaga
     9481 gccagtggta tcaggtgatg aattcgtggc cgtccaaatg gagtaacaac gactacaagc
     9541 tcacccgccc ggtggatcag gtgtcgtgga atgatatccg cggcggcgta tggccgggag
     9601 tcccgagggg tagcggattg cccggcgcag aatcgtttgt tgccaagctg agagcgaggg
     9661 ctgcaatgcc cgggttggat ctgccaacgg acgcacagtg ggaatacgcc tgccgggcgg
     9721 gcacatccgg ggcgctaaac gacggcacgg tgaacatcac caacacggac tccgacgctc
     9781 gaatggatct ggtgggccgt taccagtaca atggcggcaa ggtattgaat aacggtacgt
     9841 ggagcgatcc cccgagggac tgcacaacag cgaatgctac ggcaacggtc ggctcgtacg
     9901 cgccgaacac atggggcttg tatgacatgc acggcaactt gtgggagtgg tgtctggact
     9961 ggctcgttga tcaccttggt acggattcag ttgaagatcc gatcgggccg gattcgggtt
    10021 cagtacgcgt gatacggggc ggaagttggg gtagcaccgc gacgggttgc cgttcggcgt
    10081 atcgccggtg gaacgatccg tcgaccgagt tcagtggcta cggcttccgc ctcgttagaa
    10141 ccttgccata gcgcggtggc gctaccctta ccccggaaac ctctcccagt gagaaaagaa
    10201 gagaatcaga ccgggggtgg ggggcgggcg gtgttttgga atcaactcgg tcgggcaggt
    10261 cacgcctgtc cgacggctcc gacaaggaaa ggaaaggaca atgagacaga tgacaaggat
    10321 ggcggctttt gtcgcgatgg tactactggc ggtaaggatt ggtgccatgg atccggtgag
    10381 cgaggtgtcg gcggcagcgc cgctggacac gcggtcgatt ttcgtggaaa gggccgcaga
    10441 ggatcagaca ctgaactcgc gcagttttga cgtggactgg tccgaaatgc gcaagctcaa
    10501 cacgaagaag atcgtaggca cggtgatgct gatgcggtga tcgggcgggc cgaccccgag
    10561 gaaacgccca actttcaaga aagaaagaga acgtttttgt cccgctgaaa aagaacatcg
    10621 gaacagcaat ctacgtcagg tgatgaaatg aacagagttt ttgcagtcgc gttggtcggt
    10681 atggcggtgg gaacaacggg ggcgggaacg cttcaactcg caccgccgtt cacgaagggc
    10741 atggtgttgc agcgtgaccg tacagttccg gtatggggac gtacggcacc cggagcggcc
    10801 gtgagcgttg agtttggcgg tgagaccgtg cggacggtcg gcgggaagga tggcgcgtgg
    10861 cgcgtggacc ttccgccgca ggcagcctca aagacaccgc ggaagctgac ggtgaaggcg
    10921 gacggtgaga cacgcgttgt cgaggatgtt cttgcgggtg aggtgtggct ctgttccggg
    10981 cagagcaaca tgcacatgcc gatcgcgctg cgtcagccac gttacgggga cggacacggc
    11041 gcgatggtcg cgcaggttac gcgcaagccg ttcatccgct atgcggaggc gccttgtggc
    11101 ggaggttggc acgcactgac acctgatttt ctgcgaagcg gctggaaatg tgcgctgcca
    11161 gtccactacg cgctcgaact gtacgcgcag atcgatgtcc cgatcggtat cgtggtggcg
    11221 gcgagcggcg gcagcaacat cgacagttgg aacccggcga acggaccgaa ggccgggctc
    11281 taccatgcag ccatcgcgcg tctcgcgccg ttcggcctgc gtggtgtgat ctggtatcag
    11341 ggcgagagca atgtccacga gagttggctc tatacgcgca agttgcacga gctttacgac
    11401 ggctggtcaa aggctttcgg taatccgaac ctgcgcttcg acctcgtgca gatcgcgccc
    11461 tatgcatatg gcgcggacat gaatggatta aaagaccgcg agtttgcgcg ttttcaggag
    11521 gcgcaaatga agtttgcgga cgaagagccg aacgcgtccg tcacggtcat caatgacctc
    11581 ggcgacctgc gcgacatcca tccgaacaac aagattctcg tggcgaaacg cctcgcgcta
    11641 cacgcactca agcgcgacta cggtttctcg gacatagagg ccgattcccc gacccccgcc
    11701 gaggcgaccg ttgtttccaa ccgtgtgcgg atcgtcttcg accacgcgaa ggacctctac
    11761 gtctacaacg ccgaacgcac gctggaggcg gcgttcgagc tatgcggtgc ggacggcgtg
    11821 tggacgaagg cgcagatcgt caactttgcg aaaaccggct gggcacagtt cggaaacatc
    11881 gatgggaacg cagtcgagct tgaggcaccc ggcgtgttac agcccatcaa ggtgcggcac
    11941 gccttcacga gtccgtggaa ggggtgcctc tacaatcagg tgaacctgcc gtgctcgacc
    12001 ttcctgcggt cgctgccgtt cacccggatg aacgagacgt atcgtccaca gtttcatttc
    12061 actacgccgt cagggtggat caacgatccg aacggactcg tctggaagga cggcgtgtgg
    12121 catctctgct atcagcacaa ccgagagaag gcggactggg gaccgatgat gttctgggga
    12181 catgcggtct cgacggatct cgtgcgctgg ctcgaccgtg gtgacgtgat ctcgccggtg
    12241 aacgggcagg cctgttacag cggatcctct gcggtcgacc acgaaaacac ggcggggttc
    12301 ggcgcggggg cgatcgtatc gatgttcacg cgcacggggt cggggtcgga tctcgcctgg
    12361 tctacggacg gcgagacatg gaacctttat gaaaagaatc cggcgttgcc acggctcgcc
    12421 tgcacacacc gcgatccgat gatcgtcttt cacaagccgt ccggcaaatg ggtgctggtg
    12481 atctacacgg aaaaggaaaa tgtgtacggc gtaacgtttt tcacgtcgcc ggatctgaaa
    12541 tcctggaagg aagtcgccga catcaggggc gatccgcgcg ggaaaaagcc ggtcggtgtg
    12601 tggggaaagg accgcgactg gcttttcgag tgtccaaact tcgtcgaggt gccggtggcg
    12661 ggaacgtctg aaaggcgctg ggtgctgttc gatgcgtcgt gcaactatgc ggttgtcgat
    12721 ttcgacggcg aacaattcaa aatcgcggag ggtccgtacg aatccctgcc cacgcgcgag
    12781 aaactctact atgcggggca gacattttcg aacgcgccag acggacgctg tgtctggatg
    12841 gcttggtatc atctgaatat cccgcacccg acgttgttca cgcaactgat gagcgtaccg
    12901 tgcgacctcg cgctcatgca gacaccggga ggactgcgtc tgcggcggac gccgtcaaag
    12961 gaactcgaga ccttaaggat gggttcggca aagccgctgg aggagatcga aggggatgtg
    13021 ctcgaagtgt cgctcgactg tgtggtggtg tccggcgcga aggcggcgat cagcctgcgc
    13081 ggcgtggatc tcgtgtacga cggtgcctcg aagacgcttg cctgcgcggg ggagacaatc
    13141 gactggccgc tgcaggacgg acgttttcgt gcgcgggttt tcatcgaccg agcaggcttc
    13201 gagatcttct cggaggatgg tctcatcacg ttcccgcgcc gtgtggtgtg caacccgaag
    13261 gaccggaaac ttgtggcatc gcccgcaaca ggcgtaacag atgtgcgtca tcgtgcctgg
    13321 tgtctcgtgc gttgattggc tatgtggttt cgctttctgc cgcaaacctg tttttcggta
    13381 tgtatgatgg aaagacactc gaatgaaaaa aagtatgact gttatcgtcg ccggactcgg
    13441 tttcgttggc gcggtttgcg cggcggaaac gcctgcggtt catttgatcc ctaacgcgct
    13501 tgaaacgcgt ccgatcacaa caacagccga cgtgattctc gccaaaggat tcacggtgac
    13561 acttgaggtt gacttcgacg gtgtgggcga tgacgaaacg ctttatgagg caggcgccgt
    13621 gaaactcgcg ttccgtctgg cgggaaaaac aaaagggctg g
//
LOCUS       DAOSRB010000063        13636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4591_length_13636_cov_6.586039, whole genome shotgun sequence.
ACCESSION   DAOSRB010000063 DAOSRB010000000
VERSION     DAOSRB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4591_length_13636_cov_6.586039"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(478..1989)
                     /gene="cls"
                     /locus_tag="PLZ74_05000"
     CDS             complement(478..1989)
                     /gene="cls"
                     /locus_tag="PLZ74_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOR97713.1"
                     /translation="MMICLAAQSIDLNTSLGYGTILHYTLSLLVFFHLMLKPRDARTS
                     LLWIFFTTAFPFFGPLAYVLFGINTVPNKGWQKQYSDSLFEKRQRLNLRQSHPLASMA
                     AQRNTLQITSPDLEALPFNRVLDHLSNNHPLLGGNTIELLEPAGDALEEMFSAIQQAT
                     DHIHLATYILNDDAVGRRLMALLAERAAAGIKVRVLYDAFGSAGASLRLFFWRYRRVP
                     NLHLIGFSQANVFKRKFQLNLRNHRKILVIDGTVAFTGGINFHDVYLPRHDKPGTLDY
                     HFRIRGPAVLELQYTFLRDWYYMTDIPPEKLLAARFFPTPEPTGNCAVRLQNSGPTRD
                     ETAAALDTYFAAINLARSQILLVTPYFVPPEALIRALRQAAFRGVEVKVLVPSVNNHP
                     TVQLASQALYTTLIKAGVRIFERSPPFLHAKAFLADDTIAIIGSANLDSRSLYLNYET
                     NLVVFDTEFAARLKRVILHDFAKSREILYSNWRHRSRLRRLGENFFNLFHPIA"
     gene            complement(1986..2426)
                     /locus_tag="PLZ74_05005"
     CDS             complement(1986..2426)
                     /locus_tag="PLZ74_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97714.1"
                     /translation="MRHHRVEQWEARLNALLKRVDHALEERFGDLLTPHPARPPHGTT
                     ANPQYDGLFRVTAAFTAGFGSSLGKGYVLSIEPVSLEPLPKKARAKIEATAVQMIQEG
                     LEEALPGRGLRVERDGTVWKIVGDLSLDNPQQRAAAHPPQKENT"
     gene            2532..5600
                     /locus_tag="PLZ74_05010"
     CDS             2532..5600
                     /locus_tag="PLZ74_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOR97715.1"
                     /translation="MRDYGLRVVVLVVAGLVAGAGDAAGLLKNGGFETGGTTPDGWRL
                     QDAQGGWLRQGGYAEGACVRIVNETVGNGAWVSDPVGFKPGHCYRLTLRARGEDASGG
                     TALYGTPFAHVDIGVPSGTWERYGHVFAVPFGKEWRDVPVRLGMWQMQGQVLFDDARL
                     VPVEPVYALSEGLALGEGERLEAGRYHFVAPFRSEGRNHSRPLLGFSARFNTDRWCFD
                     AGTVVTYRHTVTGYVMRTASVSVNSGYYASGRLVVEFSADGKTWQQGGALTNAGSLTV
                     AVPEAMLPAAALSVRLRGAKPPCAVQVHGYRFDAEVEGPPRSFIGSTRYVETVSREPN
                     PRVRVIGLGDALPGGENVAVLQVRHSGAGPLTNEARVVFAGKGQPVRPHPVPIILPGP
                     GAYDVRIPYEAPSAGEWEMVVGLGEAFEARCAVTVPMLYDDSYGERLPVNVPLLSLWR
                     AASGWKIPRHRPLPRAFAKSLALRLARNEGEAVQLVVTPHVTLTNLTVTVTDLVYGKN
                     RIPASRVRVSRVGYVPVEQTSDAAGVRGLWPDPLLPQQGSLIAAAGQHHPFWIRVHAP
                     DDIPAGIYRGTVTIQADGVKATTVLNAEVYDFTLPDTMTCETAFGFSASTVWRYHKAT
                     GPEQRRAVLDLYLRCLGEYRLSPYDPAPMDRWSVTWRGFDTRTGKPEPGKKIAEIEPV
                     FHWEEWDAAMTRAFESNHFNTFRIKVEGLGCGTFHARTEPVFLGFAGGTPEYELLMGK
                     YLRGIEAHLREKGWLDKAFVYWFDEPDPKDYDFVMNGMNTLKRHAPGLRRMLTEQIEP
                     KLFGGPHIWCPLTPSLKKTGLAERREAGDHFWWYICCVPKAPYVTEFIDHAGPALRVW
                     LWQTWAERVAGVLIWETTYWTSDTAYPESGKPQNPYTDPMSWTTGYGVPKGTKRPWGN
                     GDGRLLYPPLAAADGNPAGPVMDAPVPSLRLEMLCDGIEDYEYFVILQRLLQQKGGSL
                     DPRTRQNLEALLTVPAEVSAGLTDFTRDPAPIETHRDKLARAIVFLLRLR"
     gene            complement(5663..6430)
                     /gene="kduD"
                     /locus_tag="PLZ74_05015"
     CDS             complement(5663..6430)
                     /gene="kduD"
                     /locus_tag="PLZ74_05015"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HOR97716.1"
                     /translation="MILDQFKLDGKVALVTGSARGIGQACAIALAQAGADIALVYNRT
                     PADETAKAIRSTGRRAESFQADLSAGVDVAQELVTRVAEAFDGVDILVNNAGIIRRAP
                     FAEHSVKDWEDVLAVNLSAPFYLSQAVARRMIAQGRGGKIIHIASMLSFQGGVLVPGY
                     AASKGGIRSLTMLMANELAPHNICTNAIAPGYIATENTRPLRENPARNQAILSRIPAG
                     RWGTPEDLMAAVVFLAAPASDYMNGHTLAVDGGWLAR"
     gene            6617..8989
                     /locus_tag="PLZ74_05020"
     CDS             6617..8989
                     /locus_tag="PLZ74_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97717.1"
                     /translation="MRRWVSPRLTQAAGYPSLTRRCCAWVTLFTALSATVPTARGEED
                     RNFEALPLQVQYRVLLEPWIRAAMTEIRFLQDDMAVYGLGQSGHWHMQAHDTAFCAFA
                     VLGTDPQTDEARVGMTKAELRRIALAMLRYTLRSHHSGGGVCTDGKSWGHTWISSLGL
                     ERMSVGIDALEACLDETLRALYAKVCASEADYLLTRPVTAGLIRNNHPESNQWQGNVT
                     HRAAVLNPGHPHAADWREQASVLLVNSLSTPSDETRSALVDGKPVSARFVGANMFDSR
                     ACNHHGYLNVGYMYITQSNLGIYHFACKRRGVRPPEALYHNALEQWRVNKACTFDDGR
                     LARLGGDSRVRYAYGQDYAIPVWLLARDVFGDADAERFERGWLRQVRAEQKANSDGWF
                     MRERLSGLQALSPLYFQRLEGDKACTLAYGAYWRRVFDFDAMPRRSVDAPTRWQDAHH
                     GAWLQRGTRRFASWVWHGAMKACGLCVPSDRSDMAEWQHNLAGQVCGMGAQNVAVPVT
                     WTGYAYEGGFATCGRAELISKNGIAEGSEVPGLGQIDLAFCALPDDRTTVVIQRAVTR
                     NPCYLLRVRGFSLSVPNDALNGFTRTLQSAAGRRVLRALPDRFEIVSPEGRWLNLDGR
                     LGVVELYGGAPHVARLPERQIPLNIWNSAGPKRGGGYLYCEEIGQNIREGAAYFGARA
                     TIFDCAAAVVVADAADTAAIAADRETGAVPLADADLRAVRVRDARGTLWGLLANFSTE
                     AKSLDSEPVSGWLGSGAVPLHAATHQIAPHGIAVWRLAGQGDVTTAGVRP"
     gene            complement(9090..10226)
                     /locus_tag="PLZ74_05025"
     CDS             complement(9090..10226)
                     /locus_tag="PLZ74_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOR97718.1"
                     /translation="MGWVTACVTPKFSGLDMSLGTLPRLSEAETRSISPENFTGAKGA
                     GAQSTNGPAWRAARDLGQGWKVSPFVNVPAKSTVTLADIAGSGAIQQIWMTPAPLDKT
                     RLNILRFYWDGETSPSVEVPLGDFFACGWGKYCQINSTPVCVNPGSAFNCYWVMPFRK
                     GAKVTLENLDENPMRLYYQINYTLTDVPADAATFHAQFRRVPRVGERPEVTILDGVRG
                     HGHYVGTYLAWEVRDPGWWGEGEIKFFLDGDETFPTICGTGTEDYFCGSYNFENRETK
                     RYQTFSTPHSGLAQVLPPDVIYRTGQRFGLYRWHVVDPIRFAKDLKVTIQTLGWMRDK
                     SGRYLPLHDDVASVAYWYQTEPHAPFPALPPPEVLKVGPLQVAP"
     gene            complement(10271..11722)
                     /locus_tag="PLZ74_05030"
     CDS             complement(10271..11722)
                     /locus_tag="PLZ74_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOR97719.1"
                     /translation="MTSSPTPAYAPVTPAIVDAVRAIVGPKQVVTDEEKLQAYSHDET
                     NAEEYGRMPELVVFPTSTEQVAAVVRLANRERIPITPRGAGSGLSGGAIPQFGGIVIS
                     LEKMNRLIELDTANMTVTVEAGMVTNDLAALVQEKGLFFAGYPMSLQTCMIGGNIAEN
                     AGGGKAVKYGVTGRYVTGLELVTPTGECVWLGGKLAKDVTGYDLKQLVIGSEGTLGIV
                     TQATIRLIGYPTAKSDLLVLFRTPREAIDMVPIIMSKGLVPTSIEFMDRLSILTSCRY
                     LNESLPYEAAGAMLLIETDGSHPGQVEQDLIAIGDLCEEHGAIEVFVAEDRNTIERIW
                     NVRRNIAEAFKVYSPIQSLEDIVVPPSRIPEIIPELEKLSAKYGMRIPCYGHAGDGNL
                     HATLVKDPDMPMEAWHTAEKACLNDLYRITRRLGGKISGEHGIGIKRKAYLSDLIDPV
                     ELNLMRAIKRAWDPNHIMNPGKIFDLTSQIDES"
     gene            complement(11724..12725)
                     /locus_tag="PLZ74_05035"
     CDS             complement(11724..12725)
                     /locus_tag="PLZ74_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOR97720.1"
                     /translation="MSGVWTLAEQAHGELKGVSFELLARGRKLADQLGVPLVSVLIGD
                     GVEDSRLHELIARGADEVYAVQDPRLAHFVCETYARILTHLINERKPDIILAAATSSG
                     RTLMPYVAVRVRAGLTADCTELDIEEGTGNLLQTRPAIGGNIMATIKTPHHRPQMATV
                     RPKSTRPLPPDPTRRGTIMSIPLDASLVDPRVTVLGYRRDVSDFVNLEESDIVVAGGK
                     GLKKGEHFAMLRRLAETLGGVVGASREAVDRGWIGYPHQIGLSGKTISPKLYLGAGIS
                     GSIQHLAGIKTSEIIVSVNPDPDAPLHQVADYAIVGDAFQILPALQRRLEARKGKKA"
     gene            complement(12722..13534)
                     /locus_tag="PLZ74_05040"
     CDS             complement(12722..13534)
                     /locus_tag="PLZ74_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOR97721.1"
                     /translation="MKIIVPIKQVPETSNVKMDPETGTVIRAGIETVVNPLDLYALEA
                     ALRLKEQYGGTVTAITMGPPPAVKALKEAVAMGCDNAALVSDRAFGGADTWATSYTLA
                     QAILHMGAFDLILTGERATDGDTAQVGPGIAAWLDLPVATYVARIEGIAKGRLTLERL
                     VEEGYQQLSVPLPCLLTVVKEIAAPRLPTLRGKKAAMRMEIPVFSTANLPLDSGCIGL
                     KGSPTKVVRINTPKVTRGGRKVLATDDGAVEAAVDGLIAFLDEKGLIEGERQ"
BASE COUNT         2441 a         3918 c         4596 g         2681 t
ORIGIN      
        1 cgagcgacct gcggtgccgg aaacgcgggc gtccctgtcg atcgtcgcgg gtcgacgatc
       61 ccgatcccga taccgatagc gatcccgatc ccgatttgga tgaaaccatg aaacggaacc
      121 ttcatgaaaa gcagggttgg tgttcaaagc gcaatgctgt ttgtggccgg attcgtaacg
      181 cgacaaggtc tgtatggttc aggtgccagc ggacggcgcc atgtcctttt gcggctgaac
      241 gtcggcgtga accgtagacg atccctgcca gtacggcggg acgtccacag ctgcgcgccg
      301 taggcgcgga gccggaccgc tgaaaccgtg cgggctccgg gtaacgatgt ttcaggccgg
      361 gaaggacaag catctgcttg cccggtcgag caggcacccg tcccgagcat tttcatttca
      421 tgaggtttca tgtgtttcat ggtacggaac ctaaaatcca gaaaccaatt tacctcgtca
      481 agcgatggga tggaaaagat tgaaaaagtt ttcgcccaac cggcggagcc gcgaacggtg
      541 ccgccagttc gaatagagga tctcgcggga tttggcgaaa tcgtgcagga tgacgcgctt
      601 gagacgggcg gcgaattccg tgtcgaagac gaccaggttg gtctcgtagt tgaggtaaag
      661 actgcgcgag tcgagattcg cgctgccgat aatcgcgatc gtgtcgtcag ccagaaacgc
      721 tttggcgtgg aggaaggggg gggaacgctc aaagatgcgt acgcctgctt tgatgagagt
      781 ggtatacagg gcttgcgagg caagttgcac ggtggggtgg ttgttgaccg acggcaccag
      841 tactttgacc tccacgccgc ggaaagccgc ctgacgcaat gcgcgaatca gcgcttcggg
      901 cggcacgaaa tagggggtga ccagcaggat ctggcttcgg gcgagattga ttgccgcgaa
      961 gtacgtatcg agcgcggcag cggtttcgtc gcgggtgggg ccgctgtttt gcaggcgcac
     1021 ggcgcaattc cccgtgggtt ccggcgtggg gaaaaagcgg gccgcgagaa gcttttccgg
     1081 cgggatgtcg gtcatgtaat accagtcgcg caaaaatgtg tactggagtt ccagcacggc
     1141 cgggccgcgg atgcggaagt gataatccag cgtgcccggt ttgtcgtgcc ggggcagata
     1201 cacatcatga aagttaatgc cgcccgtgaa ggccaccgtg ccgtcgatca ccagaatttt
     1261 ccggtggttg cgcaggttga gttggaactt gcgtttgaac acgttcgctt gggaaaaccc
     1321 gatcagatgc aggttgggca cccgccggta acgccagaag aagaggcgca ggctcgcgcc
     1381 tgccgaaccg aacgcgtcat acaaaacccg gaccttgatg ccggcggcgg cgcgctccgc
     1441 gagcaaagcc atgaggcgcc gtcccacggc gtcgtcattc agaatgtagg tggcgagatg
     1501 aatgtggtcg gtcgcctgct ggatcgcgga aaacatctct tccagggcgt cccccgctgg
     1561 ttcgagtaat tcgatcgtgt ttccccccaa cagggggtga ttgttgctca ggtggtcgag
     1621 aacgcggttg aacgggagtg cttcgagatc gggcgatgtg atctgcaagg tgttcctctg
     1681 ggcggccatc gaggccagcg gatgggattg gcgcaggttg aggcgctggc gtttttcgaa
     1741 gaggctgtcg gaatactgtt tctgccaccc tttgttgggc acggtgttga tgccgaagag
     1801 cacgtacgcg agcggtccga aaaagggaaa cgcggtggtg aaaaaaatcc agagcaggga
     1861 ggtgcgcgca tcccgcggct tgagcatcag gtggaaaaaa accagcagcg acagcgtata
     1921 atgcagaatc gtgccgtatc cgagcgacgt gttgaggtct atgctttgcg cggccaggca
     1981 aatcatcatg tgttctcctt ctgcggggga tgcgcggcgg cgcgctgctg ggggttgtca
     2041 agcgagaggt ctcccacgat cttccaaacg gtcccgtcgc gttcgacccg caagccgcgt
     2101 cccggcaacg cctcctccaa accctcctga atcatttgca ccgcggtcgc ctcgattttg
     2161 gcgcgcgcct tctttggcag gggttccagc gagaccggtt ctatggagag gacatagccc
     2221 ttgcctaaac tggaaccgaa ccctgcggtg aatgcggcgg tgacacggaa gagtccatca
     2281 tactgcggat tggccgtggt gccgtgcggc gggcgtgcgg ggtggggcgt cagcaggtca
     2341 ccgaaccgct cttccagcgc atggtccacc cgcttcagca atgcgttcaa ccgtgcttcc
     2401 cactgctcga ctcggtgatg tctcatccga ttatcatatc ataatccgta tgtcgcttta
     2461 aatgtgattc ttgtttccgg aggcatttct gacgtatact gcggaaagga atgcaacgtt
     2521 gggaggggac gatgagggat tacggtttgc gagtcgtggt gttggtcgtg gccggtctgg
     2581 ttgcgggggc aggggatgcg gccgggctgc tgaaaaacgg ggggttcgag acgggtggaa
     2641 cgacgcccga cggctggcgt ctgcaggatg cgcaaggcgg gtggttgcgg cagggcggat
     2701 acgccgaggg tgcctgtgtc cggatcgtaa acgaaaccgt cggcaacggg gcgtgggtgt
     2761 ccgatccggt cggattcaaa cccggccact gttaccggtt gacgttacgt gcgcgggggg
     2821 aagatgcgtc cggcggaacg gcgctctacg gtacgccctt cgcgcatgtg gatatcggcg
     2881 ttccaagcgg cacatgggag cgctacggcc atgtcttcgc cgtgccgttc gggaaagagt
     2941 ggcgcgacgt gccagtgcgt ctgggaatgt ggcagatgca ggggcaggtg ctgttcgatg
     3001 acgcgcgtct ggttccggtc gagccggttt atgctttgtc ggaggggctt gcgctgggtg
     3061 agggcgaacg gcttgaggcg gggcgttacc attttgtcgc gccgtttcgg agcgagggac
     3121 ggaaccacag tcgcccgctg ttaggttttt ccgcacgttt caacaccgac cggtggtgct
     3181 tcgatgcggg aacggtcgtg acgtaccggc acacggtgac ggggtacgtg atgcggacgg
     3241 cgagcgtgtc ggtcaacagc ggatattatg cgtcagggcg gctcgtggtc gagttttcgg
     3301 ctgacggcaa gacgtggcag caggggggcg cgctcacgaa tgcggggagc ctgacggtgg
     3361 ctgtgccgga agccatgttg cctgcggcgg cgctttcggt ccggttgcgc ggggcgaagc
     3421 ccccgtgtgc ggtgcaggtc cacggctatc gttttgatgc ggaggtggag gggccgccgc
     3481 gttcgttcat cggttccacg cgatatgtgg agactgtatc gcgtgagccg aacccgcgcg
     3541 tgcgggtgat cgggttgggt gacgcgctgc cgggcggcga gaacgtcgcg gtgttgcagg
     3601 tgcgtcattc gggtgcgggt cccctgacga acgaagcgcg ggtcgtgttc gcgggtaagg
     3661 gccaacccgt gcgcccccat cccgtgccca taatattgcc ggggcccggg gcatatgacg
     3721 tgcggatccc ctatgaggcg ccttcggccg gcgagtggga gatggttgtc ggattggggg
     3781 aggcgtttga ggcgcgttgc gccgtgacgg tgcccatgct ctatgacgac tcctatgggg
     3841 aacggttgcc ggtcaacgtt ccgttgctca gcctatggcg tgccgcatcc gggtggaaga
     3901 tcccccgcca ccggccgttg ccgcgcgcct tcgccaaatc gctcgcgttg cgtctggcgc
     3961 gtaacgaagg cgaggcggtg caactggtgg tcacgcccca tgtgacgctc acgaatctga
     4021 cggtcacggt gacggatctg gtttacggga agaaccgcat tccggcttcg cgggtgcggg
     4081 tgtcgcgcgt gggatatgtg ccggttgaac agacgagcga cgcggccggg gtgagggggt
     4141 tatggcctga tccgctcctg ccgcaacagg gatcgctgat tgccgcggcg ggacaacatc
     4201 acccgttctg gatccgggtt cacgcgcccg atgacatccc ggcgggcatc taccgcggga
     4261 cggtgacgat ccaagccgac ggggtcaagg ccactacggt tttgaatgcc gaggtgtatg
     4321 atttcacgct gccggacacg atgacttgcg aaaccgcttt cgggttcagc gcgagcacgg
     4381 tctggcggta tcacaaggca accggtcccg agcagcgtcg cgcggtcctc gatttgtatc
     4441 tgcgttgcct gggcgaatac cgcctctccc cctacgatcc ggccccgatg gaccggtgga
     4501 gtgtgacatg gcgggggttc gacacccgga caggaaaacc ggagccgggc aagaagatcg
     4561 cggagatcga accggtcttc cactgggagg aatgggatgc ggccatgacc cgtgcgtttg
     4621 aatccaacca cttcaacacc ttccggatca aggtcgaggg gttggggtgc gggaccttcc
     4681 atgcgcgcac ggaacctgtg tttctcggct tcgctggggg gacgcccgaa tatgagttgc
     4741 tgatgggtaa atatctgcgg ggcatcgagg cgcatctccg ggaaaaagga tggttggaca
     4801 aagcctttgt ttactggttc gacgagccgg atcccaagga ttacgacttt gtgatgaacg
     4861 gcatgaacac gctcaagcgc cacgcacccg gactgcgccg gatgctgacg gagcagatcg
     4921 agccgaaact gttcggcgga ccgcacatct ggtgcccgct cacaccgtcg ctcaagaaaa
     4981 cgggtctggc ggagcggcgc gaggcgggcg accacttctg gtggtacatc tgttgcgtac
     5041 ccaaagcgcc gtatgtgacg gaattcattg atcatgcggg gccggcattg cgcgtttggc
     5101 tctggcagac ctgggctgag cgggttgcgg gtgtcctgat ctgggagacg acctattgga
     5161 ccagcgacac cgcgtacccg gagtccggca aaccgcagaa cccctacacg gatccgatga
     5221 gctggaccac cggatacggt gtgcccaagg ggaccaagcg gccctggggc aatggggacg
     5281 gccggcttct ctatccgccg cttgcggcgg cggacggcaa tcccgccgga ccggtgatgg
     5341 acgctccggt gccgagtctg cgtctggaga tgttgtgcga cggaatcgag gactacgaat
     5401 atttcgtgat cctgcagcgg ctgttgcaac agaagggcgg gagtctggat ccccgaaccc
     5461 gccagaatct ggaagcgttg ctgaccgtgc ccgccgaggt gtcggcaggc ctgaccgact
     5521 ttacacgcga tcccgcgccg attgaaacgc accgtgacaa actcgcccgc gcaatcgtct
     5581 tcctgctcag gctccggtaa gcctaaaccg aaccgttgaa cgttcaacgc tcgttggttt
     5641 caggttcctc gatccggagc ggctatcggg ccagccagcc gccgtccacc gcgagtgtgt
     5701 gaccgttcat atagtctgag gcgggggccg ccagaaaaac caccgccgcc atgaggtctt
     5761 cgggcgtgcc ccaacggcca gccggaatcc gcgacaggat cgcctgattg cgggccggat
     5821 tctcgcggag ggggcgcgtg ttctccgtcg cgatataacc gggcgcgatg gcgttggtgc
     5881 agatgttgtg gggggcgagt tcattggcca tcagcatggt gaggctgcgg atgccgcctt
     5941 tggaggcggc ataccccggc accaggacgc ctccctgaaa cgagagcatc gaggcgatgt
     6001 ggatgatctt gcctccccgg ccctgcgcga tcatgcggcg cgcgacggcc tggctgaggt
     6061 agaacggagc actcaggttc accgcaagca catcttccca gtctttcacg ctatgctcgg
     6121 caaagggggc gcgccggata atgcccgcgt tgttcacgag gatgtccaca ccatcaaacg
     6181 cctccgcaac gcgggttacc agttcttgcg ccacgtccac gccggcactc aggtcggcct
     6241 gaaacgactc ggcccgtctt ccggttgaac ggatcgcttt tgcggtctcg tccgcaggtg
     6301 tgcggttgta gaccagtgcg atatccgcgc cggcttgggc gagcgcgatg gcgcaggcct
     6361 gtccaattcc gcgggcgctt ccggtgacga gagcgacttt gccgtcaagt ttgaattggt
     6421 cgagtatcat ttcggaaccc ctttcgattc agtacgggtg gagaaaatta cggaaacagt
     6481 gtgagtgtac ccgcttttca cgcggtttca agtcgaaacc ggttgccaaa acggcgtcaa
     6541 accgcaacac tatgctttgg actgcaggtg aaggagaaca cagggcaagg cccgcatgca
     6601 gtctgagggg gggagcatga ggagatgggt ttcgccgcgg ttgacacaag cggccgggta
     6661 tccaagcctg accagacgct gttgcgcgtg ggtcactctg ttcactgcgc tgagtgcaac
     6721 cgtgccgacg gcacggggag aagaggaccg gaacttcgag gcgttgccgt tgcaggtgca
     6781 gtaccgcgtt ctgcttgagc cgtggatccg tgcggccatg acggagatcc gttttctgca
     6841 ggacgatatg gccgtgtacg gcttgggaca gtccggacac tggcacatgc aggcgcacga
     6901 taccgcgttc tgcgcatttg ccgtgctcgg aaccgatccg caaaccgatg aggcccgggt
     6961 cggaatgacg aaagccgagt tgcggcggat cgcgttggcc atgttgcgtt acacgctccg
     7021 ctcgcatcat tccggaggcg gcgtgtgtac ggacggcaag tcgtggggac acacttggat
     7081 atccagcttg ggactcgaac ggatgagcgt gggaatcgac gcgctggagg cgtgtctcga
     7141 cgagacgttg cgcgcgttgt acgccaaagt ctgtgcctct gaagcggact atctgttgac
     7201 gcgtccggtc acggcgggcc tgatccggaa caatcacccc gaatcgaatc agtggcaggg
     7261 taatgtcaca caccgggcgg cggtgttgaa tcccggtcat ccccatgcgg ccgattggcg
     7321 cgaacaggcc agcgtgctgc tggtcaactc gctttccact ccctcggatg aaacccggtc
     7381 ggcgcttgtg gacggcaaac ccgtctctgc gcggtttgtc ggggccaaca tgttcgactc
     7441 gcgcgcctgc aaccaccacg ggtatctcaa tgtgggctac atgtacatca cgcagtccaa
     7501 tctcgggatc taccattttg cctgcaaacg gcgcggcgtg cgtccgcccg aggcgctcta
     7561 tcacaatgct ctggagcagt ggcgtgtgaa caaggcgtgt acctttgatg acgggaggtt
     7621 ggcgcggttg ggcggcgaca gccgggtgcg ttatgcctat ggacaggact atgcgatccc
     7681 ggtctggctg ctggcgcggg acgtgtttgg tgacgcggat gcggaacggt ttgagcgcgg
     7741 atggctcagg caggtccggg cggaacagaa agccaattcg gacggctggt tcatgcggga
     7801 gcgtctgagt gggttgcagg cactctcgcc gctctatttc caacgcttgg agggggacaa
     7861 ggcctgtact ctggcgtacg gtgcgtactg gcggcgggtc ttcgatttcg acgccatgcc
     7921 gcgtcggtcg gtcgacgcgc cgacccgctg gcaggacgcg caccacggcg cgtggctgca
     7981 acgggggacg cgccggtttg cctcgtgggt gtggcacggc gcgatgaaag cttgcggtct
     8041 ctgcgtgcct tcggatcgct cggatatggc cgagtggcaa cacaatctgg cgggacaggt
     8101 ctgtggaatg ggggcgcaga acgttgcggt gccggtgacg tggaccgggt atgcgtatga
     8161 gggcgggttc gcgacgtgcg ggcgtgcgga gctgatctcc aaaaacggga tcgcggaggg
     8221 cagtgaggtg ccgggtttgg ggcaaatcga tctggctttt tgtgcgcttc ccgatgaccg
     8281 gacaacggtg gtgatccagc gcgcggtcac ccgaaacccc tgttatctgc tgcgggtgcg
     8341 ggggttttcg ctctcggtcc ccaacgatgc gcttaatgga ttcacgcgga cactccagag
     8401 cgcggctggc cgccgcgtgt tgcgggcgtt gccggaccgg tttgaaatcg tttcgccgga
     8461 agggcgctgg ttgaatctgg atggacggct gggtgtggtc gaactgtacg gcggcgcgcc
     8521 gcatgtggcg cgtctgcccg agcgccagat tccgctcaac atttggaatt ccgcaggacc
     8581 caagcgcggc ggggggtatc tgtattgtga ggagatcgga cagaacatcc gcgaaggcgc
     8641 ggcctatttc ggcgcgcggg cgacgatctt cgactgcgcg gcggcggtgg tggtcgcgga
     8701 tgcggctgac acggcggcaa tcgcggcgga ccgggagacg ggcgccgttc ccttagccga
     8761 cgctgatctg cgcgcggtcc gggttcggga tgctcggggc acgctctggg gcctcctcgc
     8821 gaatttttcc acggaggcta agtcgttgga ttcggaaccg gtgagcggtt ggttgggaag
     8881 cggcgcggtc ccgctgcatg ccgcaaccca tcaaatcgcg ccgcacggca tcgccgtttg
     8941 gcggctcgcc ggacagggtg acgtgaccac cgctggcgtg cggccctagc tgcggagcgg
     9001 gtggggagtg cacaggtcga atgtcgtggg gcgcaaggcg acgttcacga ggacgagaac
     9061 gggaaaacgt gagaaacagt tccgtttcgt tacggtgcca cttgaagcgg cccgaccttc
     9121 agcacctcgg gcgggggcaa tgcgggaaaa ggcgcgtgcg gttcggtctg ataccagtaa
     9181 gccaccgagg ccacatcgtc atgcagcggc agatagcggc cggacttgtc ccgcatccat
     9241 cccagcgtct ggatcgtcac cttcaggtct ttcgcgaaac ggatcgggtc gaccacatgc
     9301 cagcggtaca gtccgaagcg ttgtcccgtc cgatagatca cgtccggcgg cagcacttgc
     9361 gccaggcccg agtgcggcgt gctgaaggtc tggtagcgtt tcgtttcccg gttttcgaaa
     9421 ttgtaggagc cgcagaaata gtcctccgtg ccggtgccgc agatcgtcgg gaacgtctcg
     9481 tcgccgtcta ggaagaactt gatctcgcct tcgccccacc agccggggtc ccggacttcc
     9541 catgcgagat aggtgcccac atagtgcccg tggccgcgca cgccgtccag gatcgtcact
     9601 tcgggacgct ctcctacgcg cggcacgcgc cggaactgcg catggaaggt cgccgcgtcg
     9661 gcgggaacat cggtcagcgt atagttgatc tgatagtaga ggcgcatcgg gttctcatcc
     9721 agattttcga gtgtaacctt ggcgcctttg cggaaaggca tgacccagta gcagttaaag
     9781 gcgctgccgg ggttgacgca caccggcgtc gagttgatct ggcaatactt accccatccg
     9841 cacgcgaaga aatcaccgag cggcacctcc accgagggcg aggtctcgcc gtcccagtag
     9901 aagcgcagga tgttgaggcg cgttttgtcg agcggcgcgg gcgtcatcca aatctgctgg
     9961 atcgcgccgg aaccggcgat gtccgccagc gtgaccgtgc ttttggctgg cacgttcacg
    10021 aacggcgaca ccttccagcc ctgtcccaga tcgcgtgccg cgcgccaagc cgggccgttg
    10081 gtcgactgcg ctcccgcgcc cttcgctccc gtgaaattct cggggctgat cgacctcgtc
    10141 tcggcttccg acaagcgggg cagcgtgccg agactcatat ccagcccgct gaacttcggt
    10201 gtcacgcacg ccgtgaccca tcccatcgcg atgaacgcca atatacggac tcgatgcatt
    10261 tttcactcct tcatgattcg tcaatctggg acgtcaggtc aaagattttt ccggggttca
    10321 tgatgtggtt cggatcccac gcgcgcttga tggcgcgcat cagattcagt tcgacgggat
    10381 cgatgaggtc gctcagatac gctttccgct tgatgccgat accgtgctcg cccgaaatct
    10441 tgccgcccaa ccgccgggta atccggtaca ggtcattcag gcaggccttt tccgcagtgt
    10501 gccacgcctc catgggcatg tccggatcct tgaccagcgt ggcatggagg ttgccgtcgc
    10561 cggcgtggcc gtaacagggg atgcgcatac cgtatttcgc gctcaatttc tccagttcgg
    10621 ggatgatctc cggaatgcgg gagggaggga ccacgatgtc ttccagactc tggatcggac
    10681 tgtagacttt gaaggcctcg gcgatgttac gccgcacgtt ccagatgcgc tctatggtat
    10741 tgcggtcctc ggccacgaac acctcgatcg cgccgtgctc ctcgcagagg tcgccgatcg
    10801 cgatcagatc ctgctccacc tgccccggat gggagccgtc cgtctcgatc agcagcatcg
    10861 cgccggccgc ttcatagggc aggctctcgt tcagataacg gcaactggtg aggatcgaga
    10921 ggcggtccat gaattcgatg ctggtgggca cgagaccctt cgacatgatg atcggcacca
    10981 tgtcgatggc ctcgcgcggg gtccggaaaa gcaccagcag atcggatttc gccgtagggt
    11041 aaccgatgag ccggatggtg gcttgggtga caatccccaa ggtgccttca gaaccgatga
    11101 cgagttgttt gaggtcgtac ccggtgacgt ccttcgcaag cttgccgcca agccatacgc
    11161 actcgccggt cggcgtgacc agttccagtc cggtgacgta acggccggtc acgccgtatt
    11221 tgaccgcttt gccgccgccc gcgttctccg cgatattgcc gccgatcatg caggtttgca
    11281 gactcatcgg gtatccggcg aaaaacaggc ctttttcctg caccaacgcg gcgaggtcat
    11341 tggtgaccat cccggcctcc acggtgacgg tcatgttggc ggtgtccaat tcgatgaggc
    11401 ggttcatctt ctccagcgag atcacaatcc cgccgaactg cgggatggcg ccacccgaca
    11461 ggcctgagcc cgcaccacgg ggggtgatcg ggatccgctc gcggttggca aggcggacca
    11521 ctgcggccac ctgttcggtc gaggtcggaa acaccacgag ttcaggcatg cggccatatt
    11581 cttcggcgtt ggtctcatcg tgcgaatacg cctgaagctt ttcttcatcg gtcacaacct
    11641 gtttggggcc gacgatcgcg cgcacggcat cgacgatcgc gggcgtgacc ggggcataag
    11701 cgggggtggg ggaggaggtc atgttacgcc ttctttcctt tacgggcttc cagccgccgc
    11761 tgcaacgcgg gcaaaatctg aaacgcgtcg cccacgatcg cgtagtcggc gacctgatgc
    11821 agcggggcat cggggtcggg gttcacggat acgatgatct cactggtctt gatccccgcg
    11881 aggtgctgga tggaacccga gatgcccgcg ccgagataga gtttggggga gatggtttta
    11941 ccgcttaacc cgatctggtg gggatagccg atccagccgc ggtccaccgc ctcgcggctt
    12001 gcgccgacca cgccgcccaa cgtttcggcg agacgccgca gcatcgcgaa atgctcgccc
    12061 tttttcagtc ctttgccgcc cgcgaccacg atgtcggact cttccaagtt cacgaagtcc
    12121 gagacgtccc ggcggtaccc cagcacggtg acgcgcggat cgaccaggct ggcgtccagc
    12181 gggatcgaca tgatcgtgcc gcgccgggtc gggtcggggg gcagcggccg ggtggatttg
    12241 ggccggaccg tggccatctg cgggcggtga tggggggtct tgatggtggc catgatgttg
    12301 ccgccgatcg cgggccgcgt ctgaagcagg ttgccggtcc cctcctcgat gtcgagttcg
    12361 gtgcagtcgg cagtgagacc ggcgcggacg cgcacggcca catacggcat cagggtgcgc
    12421 cccgaagagg tggcggccgc cagaatgatg tcgggcttgc gctcgttaat caaatgggtc
    12481 aggatccgtg cgtaggtctc gcaaacgaaa tgggcgagcc ggggatcctg cacggcgtag
    12541 acctcatccg cgccgcgcgc gatcaactcg tgcagacgtg aatcctcgac gccgtccccg
    12601 atgaggaccg aaaccagcgg tactccgagc tggtccgcga gtttacgtcc gcgtgccagc
    12661 aattcgaaag aaaccccttt gagttcgccg tgagcctgtt cggccagcgt ccacactcca
    12721 ctcattgccg ttctccttca atcaatcctt tttcgtcaag gaacgcgatc aacccgtcca
    12781 cggccgcttc cacggcaccg tcgtccgttg ccagcacctt tcgcccgccc cgggtcactt
    12841 tgggcgtatt gatccggacc accttggtgg gggaaccctt gaggccgatg cacccggagt
    12901 cgagcggcag gttggcggtg ctgaaaaccg gaatctccat acgcatggcc gcctttttac
    12961 cgcgtagggt cggcagccgc ggcgcggcaa tctccttcac cacggtcaac aggcagggga
    13021 ggggcacgga caactgttgg tatccctcct ccaccagacg ttcgagcgtc agcctgccct
    13081 tcgcgattcc ctcgattcgg gcgacgtacg tggcaacggg gagatcgagc caagccgcga
    13141 ttcccggtcc gacttgggcg gtgtcgccgt ccgtggcgcg ttcgcctgtg agaatcaggt
    13201 cgaaggcccc catatgcagg atggcctggg cgagcgtgta cgaagtggcc caagtgtcgg
    13261 caccgccgaa cgcgcggtcg gagacaagcg cggcattgtc gcaacccatg gccaccgctt
    13321 ctttcagcgc tttgactgcg ggcggcggcc ccatggtgat ggcggtgacg gttcccccgt
    13381 actgctcctt gaggcgcagg gctgcctcca gcgcgtacaa gtccagcgga ttcaccacgg
    13441 tttcgatccc ggcccgaatc acggttccgg tttccggatc cattttgacg ttactggttt
    13501 cggggacctg tttgatcggc acgataattt tcacaagact ctccagaaaa cgggttaaac
    13561 ggagcataac aaaaaccaga ttgaagacaa tccggattat cggcttgtac cattgcgcgg
    13621 ggaggattac tattag
//
LOCUS       DAOSRB010000064        13635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4592_length_13635_cov_6.230633, whole genome shotgun sequence.
ACCESSION   DAOSRB010000064 DAOSRB010000000
VERSION     DAOSRB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13635
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4592_length_13635_cov_6.230633"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLZ74_05045"
     CDS             complement(<1..514)
                     /locus_tag="PLZ74_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97722.1"
                     /translation="MKKACPRTTVGRRFVVALAAALGIVGAAFAVPTAPTLKMTGFFN
                     GKLTFGVKYSPVTDHTLEVQMKDQGAADDDYVTIYTKAVNSLAKFLANKYDAAYFTYA
                     TNFAGTATFRMRTTLNGEASDWVAAGDFTAYVNVTGATNSNATTFGNAVDGNMYTNVD
                     EPSNPWVGYDY"
     gene            complement(773..3415)
                     /locus_tag="PLZ74_05050"
     CDS             complement(773..3415)
                     /locus_tag="PLZ74_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOR97723.1"
                     /translation="MLFGRSGFIFASLCVGFRVWAGEPVTVTFLQGFDPDRPENPGTR
                     QILRFAQREPRLNPQKWGGLELPGAGGRAPFMLSLAGGTAPDIYFCWFHIIRHDIEQG
                     FLHPLNEWLGEDLDGDGQLGDHEARWPGWKRVPPLWRQAATRDGKVYGLPMAGIWYYG
                     IVYRKDLVQQAGVDPESVPATWDEFFRWCQRLTFPGKRIAGARVQRGQRAFGAENRPW
                     GWLPWVQAAGGSPIVNLRVSPATGRTYRFPMEETRFIAPDTGEDLSGVPGVWQAAFDS
                     PEAIEAAEFFHRLAWAPWLRDPQSGEPVDLSPEALAAGCVTLPDGREHRFQPGEVIRG
                     VVRALPRLTQDLPQLFAQGEVVALFSGAELVEKLTRDLNLPPDMVGIMPFPAARPGLK
                     PVFQAHKHFYSMTEGVGRRPKAERDMVWACVEQLASEEVNDEAVRQKVLEGHARWCTP
                     TELTRLGFDEYLAEVPAGIRRNYERLEAGEILARTEPFAGFWQAVSDLLDRRFLGLLL
                     ADTGERFDVAAALREINAEANRGLMFRASETMLARRRPLARVLFGVGIITLLGCAALI
                     VRRDARDADASAVSARRIGAMRVRHGLSPWLMLAPAVLSVALWSYYPLLRGAVMAFQE
                     YKIVGETRWAGLDNFILVATDAGFWAAWGRTLRYVGLTLLLGFLTPVLLALMLAEIPR
                     GKVFFRTVYFLPHLTSAMVIALLWKLMYDPTENGMLNRILLGLGASRQTWLQDPGLAM
                     VCCILPGVWAGAGMASLIYVAALHSLPPDYYEAAALDGAGILSRFRHVTLPQILPLMV
                     INFVGAFIAAFQGMGSIFLLTFGGPGDATNVLSLAIWKEAYNNLRFSTATTMAWFLGV
                     ALIGFTYLQIRILRKVEFRRAAAN"
     gene            3800..5014
                     /gene="trpB"
                     /locus_tag="PLZ74_05055"
     CDS             3800..5014
                     /gene="trpB"
                     /locus_tag="PLZ74_05055"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOR97724.1"
                     /translation="MNVPRPLSGNPDAKGFFGKYGGQFLPPQLEKPFAEITEAYHRIA
                     NDAAFISELRLIRKHFQGRPTPVYHARKLSLQNGGAHLYLKREDLNHTGAHKINHCMG
                     EGLLARFMGKKKLIAETGAGQHGVALATAAAYFGLQCEIHMGEVDIAKQAPNVSRMKL
                     LGAKVVPVTHGLKTLKEAVDSAFEAYLADYENSIYCIGSVVGPHPFPLMVRDFQKVVG
                     IEAREQFIGMTGNLPDAVCACVGGGSNAMGIFHGFLDDPCEIYGVEPLGRGPKTGDHA
                     ATMSYGREGIIHGFKCYLLQNEDGSAAPVYSCASGLDYPGVGPEHCHLKDTGRVRYVT
                     CSDEACRDAFFKLCRLEGIIPALESAHAVAFALTQAREMESGAILVNLSGRGDKDLDY
                     VIENWGVGETEA"
     gene            complement(5178..6629)
                     /locus_tag="PLZ74_05060"
     CDS             complement(5178..6629)
                     /locus_tag="PLZ74_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOR97725.1"
                     /translation="MNKRAVFHLVSYMTLFIGLAVTACAAVSHVCGDALEVRCAFLLA
                     GGITLAVSLVAGMLTRGDLNLGRRDGFGVVTLGWLSAALFGALPYLLTGVITHPVSAL
                     FETVSGFTTTGASVLDNLENLPRGILFWRALTQWLGGMGVLVLCVAILPFLGVGGMQL
                     YRAEMPGPFKDRLTPHIATTAKLLWAVYLLLTVVQTLLLKGVGGMGWFDSVCHTFATV
                     ASGGFSTRTASVAAYDSATVEGIITLFMFLAGINFSLHLSALTGRPGHYVRDPECRAY
                     FLFLTGCCLFMTANIWATGHGSFARCLRDAFFTGTSVLTSTGFCTVDFDRWPGASRLL
                     LVFMMLMGACAGSTGGGIKVVRIMIVFKKILREIRLFMRPTAAIQVKLGPKPVEPDLV
                     LHVSAFVIIYFAVFALGSFLMTFFTPDLETATTSVITALSNVGPGLSAVGPVRNYASI
                     PLAGQGVLATCMLLGRLELYTVLVLLFPSCWRR"
     gene            complement(6640..7989)
                     /gene="trkA"
                     /locus_tag="PLZ74_05065"
     CDS             complement(6640..7989)
                     /gene="trkA"
                     /locus_tag="PLZ74_05065"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOR97726.1"
                     /translation="MNSVIIGAGHAGRLLASHLCRERHSVVMVDESAEALEQAEASLD
                     VLTVCGSGADPAILEKAGAGRADLFVAVTDRDEINIIACLLAHSEGVPRKIARVGSAQ
                     YDPGAVKYNLTKLGIDLLINQKFACAKEICNALLLPGAVESFDLFGGRVLVAGFGIVG
                     GSPILGHTPATLPDQDLVRKLRIIAIRRQGRLVIPRGDTAFLPEDVLYLVGAPDDIRV
                     FSTWMDPVREAFEKVIVAGGGDIGLAIAKNLERIGLHVVLLEQDEERALFCSGELDHT
                     LVLRADALSGSALDETGIVPGTAFVAVTGDDEDNAMNCLMARKKGADFVVTQIARTDY
                     IPIMENLGLVDRIISPYESITNAILHYLRSRDVRAAALLHDLPGELLEIELSPTSRYA
                     GKRVSDIRFPSGSIVAVVQRGDGVLPATGGLCFEAGDRLLIFADPAAVKKIQAMFRK"
     gene            8294..9472
                     /locus_tag="PLZ74_05070"
     CDS             8294..9472
                     /locus_tag="PLZ74_05070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOR97727.1"
                     /translation="MNKTSGTGWRAVLCALCTVWGAGVADAQHVTGQTKPVLPVVDLS
                     GETARHVVIAAGTPEEYQGHPTTVLLPDGKTVFCVWCINHGGAAGPMARSADGGKTWV
                     RLDTTLPPGFRTHQNCPSIYRLIDPQGKARLWVWSAAKDTRQGTPMPSIMSEDDGEMW
                     REMPPLGEAFRCVMTFSSMVRLKDGSYLGLYHRGEGKGEGRKFLEVVQSVTRDGGFTW
                     SVPRVVANVAGKMACEPFVFRSPDGSELCCLMRENTHKGHSLQMFSRDEGATWSVPED
                     TPWGLTGDRHMGVYAPDGRLVIAFRDQAPESPTKGHFVAWVGNYGDIRQRRDGQYRIK
                     LLHGYKRVDLGYPGVERLADGTLLATTYLKYWEDTRQHSVVCTRFTLAETDAKAGLNP
                     "
     gene            complement(9516..13517)
                     /locus_tag="PLZ74_05075"
     CDS             complement(9516..13517)
                     /locus_tag="PLZ74_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97728.1"
                     /translation="MRYAIPNLIHVLCVIGFFAGGLIRAEDGTVQQTGNFSTPATWVD
                     GVIPGGAGATLYVKGNYTLNNFFTNAVLGHIVTPADTPVAGLTSVFTVSLPWENRTSG
                     NPDPVYGTYRFGVFTNYLTLDSGVEGEPATIRCLPLLTSNFRYAINIPLLLKSDLEVQ
                     HNGRASGNWSNPLSAVWLGRDIREEGGRRGIRVRFDKEDMQLMLKGNNTFSGDLIVEK
                     GIVRAGDPFRYNVPGNQFGVDNTLIATSGVGTVDVNGLHFGPEKTILIGGRGANGMGA
                     LTSGCQSPGVTSVWSGPVTLVSDAAVAGTVSRYPRSEGGNLRIDGAVTDGGRGCRLEN
                     VSLRNLVLAGNNTYSGGTLLTSGFLAALTPSSLGSGPLIFNGGAFRIDSSTGVDPSAF
                     TLVSTNSKPVQLLTDGADFTLGHALTGFTSGLTKSGLGALILKQDNAYTGTTAVVLGD
                     LILDYSEAGGQKTPADGTITLGNNARLILRNGQTATPDQAVRTLSTYKGASARVIHDS
                     AMPLAFNFLTPNTDSVLNLEGEGPYKLIQTSNGSENSAYPNILPANILFKGRDWAVRT
                     ASGNAISNFTDYASDWSGSNKHVDVTAGLAQSVPANLEVATLRFNTPNNGNPIILNLS
                     GTNQIKSGAILVTEAMGNTPVHITGGAIARMGSGNLIIYNFNTQAVVRIDSQLLGFPV
                     FNTNDLGQVTGITLSDVGVMKAGPGKVILGNADNRHDDYTSVLEGELEISGPECLGVG
                     SWHGSWKSVQNFVMIYNGGTLTTTGSFDFAREVYPGYTNITTLDINSAGGVLNVSGAG
                     DRVANLDANPTPVLRNGGRLVKRGAGTWFVGRGFGSGADRAEDTVTAREYTMFFDVEE
                     GSIEHRAGTTSANSFLGEGSMSMTVRNNAVLKGGGFLYGNNGGGNGGGNPEDVAIRKV
                     LHIDAGGAIVDLNGITVNMGWSLFDGLTRTSDWLEGPGTLTITNSSATGASFRFYNLL
                     NHAFTGRFDASGLMAADATYGGGLPNGELVVPEHRVYSFTSCYPSHYPLHFGRVTGSG
                     AFGGRGISNSDSDNVALIGCDDNGVFEFEGYLWGTWGSSNRGAFRFVKAGNNTWRISG
                     TTNAMTTSVTLRKGTILVGADSPGNGTVGALGTERVILSDAGTAPGDALSLLTDGPYT
                     VGNGIILMDYAPGALMTLGGNQTTGASAFTGDLALTRDVCLHAANTDDSGVTFSGAIT
                     GPGGIIKTGPGKVILTGSVQNTAPHTVLDGTLMIMGDTTLTNALVVAAGASGSGTLAV
                     TGNLTIGEGVTLTLQPDSLVRGQTYTLATWTGTLSGSFASVTGLPDGWHVGRFPDHLE
                     LYYAPPGTQIRIR"
BASE COUNT         2749 a         4322 c         4181 g         2383 t
ORIGIN      
        1 tataatcata gccaacccac ggatttgacg gttcgtccac attcgtatac atgttgccgt
       61 cgacggcgtt accgaaggtc gtggcattgc tgttcgtggc gcccgtcaca ttcacgtagg
      121 ccgtgaaatc accggccgcc acccagtcac tcgcctctcc gttcaaagtg gtgcgcatac
      181 gaaacgtggc cgtacccgcg aaattcgttg cataggtaaa ataggcagcg tcatacttgt
      241 tcgccaggaa cttggcgagc gaattgacag cctttgtgta aatggtgacg tagtcgtcgt
      301 cggccgcgcc ctgatccttc atctgcactt ccagcgtatg atcggtaaca ggcgagtatt
      361 tcacaccaaa cgtgagtttg ccgttgaaaa aacccgtcat cttgagcgtg ggggccgtgg
      421 gcacggcaaa ggccgcgcct acaattccaa gagccgccgc caacgcgaca acgaacctgc
      481 gacctacagt tgtacgaggg catgcttttt tcatggtttc gactcctacg attttcacgt
      541 tatcttaagg tcaacgaaga tagtataccg caccctgtga cagatcgtcc agcggattcc
      601 cgcaaaactg gtaatcgcaa tcggttggtg acagaaggtg cgaagatcat gcgtttccgg
      661 ttccgggttt atcggaatcg ggaccgcttg cccgtattcg tcctcgttct cgccgacttg
      721 cggcgtttgg gttatccggc agagcgacgg atccgccgtt gcggccacag gattagttcg
      781 cggcggcgcg gcggaattcg actttacgca ggatgcggat ctggagatag gtgaacccga
      841 tcagcgcgac acccagaaac cacgccatgg tggtggccgt gctgaagcgc aggttattgt
      901 acgcctcttt ccaaatggcg aggctgagca cgttggtggc gtcgcccggc ccgccgaagg
      961 taagcaggaa gatgctgccc atgccctgaa acgcggcaat gaacgcgccc acgaaattga
     1021 tcaccatcaa cggcagaatc tgtggcaggg tgacgtggcg gaagcggctg agaatccccg
     1081 cgccgtcaag agccgcagcc tcgtaatagt cgggcggcag ggagtgcagg gccgccacat
     1141 agatcaggct cgccatgccc gcgcccgccc acacacccgg caggatgcag cacaccatcg
     1201 ccagtccggg gtcttgcagc cacgtctggc gcgaggcccc caaccccagc aggatgcggt
     1261 tgagcatgcc gttctccgtg ggatcgtaca tgagtttcca cagcagcgcg atcaccatgg
     1321 cgctggtgag gtggggcagg aaatagaccg tacggaaaaa cactttgccg cgcgggatct
     1381 ccgcgagcat cagcgccagc agcacgggcg tcagaaaccc gagcagcagg gtgaggccga
     1441 cataccgcaa ggtccggccc cacgcggccc aaaaccccgc gtccgtggcc accagaatga
     1501 agttgtccaa cccggcccaa cgggtctcgc ccacgatctt gtactcctga aacgccatca
     1561 ccgcgccgcg cagcaacgga taataactcc acagcgcgac agacagcaca gccggcgcca
     1621 gcatgagcca aggcgaaagc ccatgccgca cccgcatcgc gccgatacgg cgcgcggata
     1681 cggcggaggc gtccgcgtcc cgggcgtccc gccgcacgat cagcgccgcg cagcccagca
     1741 gcgtgatgat tcccaccccg aacagaacgc gcgcgagagg acgccgacgc gccaacatcg
     1801 tttcggaggc ccggaacatc agcccgcgat tcgcctccgc gttgatctcc cgcagggccg
     1861 ccgcaacatc gaaccgctcg ccggtgtccg ccagcagcaa ccccagaaaa cgccggtcca
     1921 gaaggtcgga caccgcctgc cagaatcccg cgaagggctc ggtacgcgcc agaatctcac
     1981 ccgcctccag ccgttcgtag ttccgacgga tgcccgcggg cacctccgca aggtactcat
     2041 cgaatcccag ccgcgtcaac tccgtaggcg tgcaccaacg cgcatgccct tccagcacct
     2101 tctgcctgac cgcctcgtcg ttcacctcct cgctggccaa ctgctccacg caggcccaca
     2161 ccatatcgcg ttcggctttg ggacggcggc ccacgccttc ggtcatgctg tagaaatgct
     2221 tgtgcgcctg aaaaacaggc ttgagtcccg gccgcgccgc cggaaagggc atgatgccca
     2281 ccatgtccgg cggcagattc agatcgcgcg tcaatttttc gaccagctcg gcaccggaga
     2341 aaagcgccac gacctcgcct tgggcgaaga gctgcggcag atcctgagtg agccgcggca
     2401 gcgcgcgcac cacgccccgg atgacctccc caggctgaaa gcggtgttcc cgcccgtccg
     2461 gcaacgtcac gcaacccgct gcgagcgctt cgggcgacag atcaaccggc tcgccgcttt
     2521 gcggatcacg cagccaggga gcccaggcca accgatggaa gaactcggcc gcctcaatcg
     2581 cctcgggcga gtcgaacgcc gcctgccaca cgcccggcac accggagaga tcctcgcctg
     2641 tgtcgggcgc gatgaaccgc gtctcttcca tggggaaacg gtaggtacgg cccgtcgcgg
     2701 gcgagacgcg gaggttgacg atcggcgagc cgcctgcggc ctgcacccaa ggcaaccagc
     2761 cccacggacg gttctccgcg ccgaacgcac gttgcccgcg ctgcactcgg gcgcccgcaa
     2821 tacgtttgcc ggggaaggtc agacgctggc accagcggaa aaactcgtcc cacgtggcgg
     2881 gcacgctttc cggatccaca ccggcctgct gcaccagatc cttgcggtac acgatgccgt
     2941 agtaccagat gccggccatc ggcagaccgt ataccttgcc gtcgcgcgtg gcggcctgac
     3001 gccacagcgg cggcacgcgc ttccagcccg gccagcgcgc ctcgtggtcg ccaagctgcc
     3061 catcgccgtc caagtcctcc ccgagccact cgttcagtgg atggaggaaa ccctgctcga
     3121 tatcatgccg gatgatatgg aaccagcaaa agtagatgtc cggcgccgtg ccgcccgcca
     3181 gcgagagcat gaacggcgcg cgcccgcccg cgccgggcag ttcaagacca ccccactttt
     3241 gcgggttgag ccgcggctcg cgctgcgcga aacgcagaat ctgccgggtc cccggattct
     3301 cgggacggtc cggatcaaag ccctgcagga acgtcaccgt caccggttcg ccggcccaca
     3361 cacggaaacc cacacaaagg ctcgcgaaaa tgaatcctga acgcccaaag agcatacgcg
     3421 aaccttaccg cacccttggc gcgaaagcaa gcgggcatgt aaacccaaca atcgcgttcg
     3481 tgaccatgaa acagatgagc cttcatgaaa acccggttcc ctgtcctcgt actcgccgac
     3541 ttgcgacccg cggcattcga cctgcggcgc ttgggttgtc cggcagggcg acggatccgc
     3601 cgttgcgggt ggagacaccc gtgttcccaa ctcccgcacg aatacacgaa tgaactctca
     3661 tcggtcagaa aacgggggtg caacgcgggt tcataaaaca cgaacggttt tcgcttgcgg
     3721 cacgcgtggg cttcaggtat attgtttctt tcaacagaaa cgattggccc ggacaccggg
     3781 ccttatcagg gagacacgca tgaacgttcc gcgccctttg tcaggcaatc ccgacgccaa
     3841 aggctttttt ggcaaatatg gcggccaatt ccttccgccg cagctcgaaa aaccctttgc
     3901 ggagatcacc gaggcctacc accgcatcgc caacgacgcg gcgttcatct ccgagctgcg
     3961 cttgatccgc aaacacttcc aaggccgtcc cacaccggtg taccatgcgc gcaagctctc
     4021 actccagaac ggcggggcgc atctttacct caagcgcgag gatctcaacc acaccggcgc
     4081 gcataagatc aaccactgta tgggcgaggg gttgctggcg cgcttcatgg gcaagaaaaa
     4141 actcatcgcg gaaaccggcg cgggccagca cggcgtggcg ctggccacgg ccgcggctta
     4201 tttcggtctg caatgcgaga tccacatggg cgaggtggac atcgccaaac aggcgcccaa
     4261 tgtcagccgc atgaaattgc tgggcgcgaa agtcgtgccg gtcacccatg gcctcaaaac
     4321 cctcaaagag gcggtggatt cggcttttga ggcctatctg gccgattacg aaaactccat
     4381 ctactgcatc ggttcggttg tcggcccgca ccccttcccg ttgatggtgc gtgatttcca
     4441 gaaagtggtc ggcatcgagg cccgtgagca gttcatcggg atgaccggca acctgcctga
     4501 cgcggtctgc gcctgtgtgg gcggcggttc taacgcgatg gggattttcc acggctttct
     4561 ggatgacccc tgcgaaatct acggggtcga gccgttggga cgcggcccga agacgggtga
     4621 ccacgcggcg acgatgtcgt acggtcgcga agggatcatc cacgggttca agtgctacct
     4681 gctgcagaac gaggacggtt cggccgcccc ggtctattcc tgcgcgagcg gactggacta
     4741 tccgggcgtg gggccggagc attgtcattt gaaggatacc ggacgcgtgc gatacgtgac
     4801 ctgttccgac gaggcgtgcc gcgatgcctt tttcaagttg tgccgactcg aggggatcat
     4861 tccggcgctc gaaagcgcgc acgccgtggc gttcgcgctg acccaggcgc gcgagatgga
     4921 aagcggggcg attctggtca atctcagcgg acgcggcgac aaggacctcg attacgtgat
     4981 cgagaattgg ggcgtcggcg aaacagaggc gtaagcttcc ggcacgggcg gcgatccggc
     5041 gtgaagaatc cctagggcgt tttcgcaaaa ggtgtagccg ttccgggtaa ggacgcctcc
     5101 ccgaggcgtc cgcggacggt tcggggaacc gcccctgcca gcaatcgaac ctcggccaca
     5161 aaacagcgta acctgcgcta ccgccgccag caactgggga acagcagaac cagcaccgtg
     5221 tacagctcga ggcgcccgag cagcatgcag gtggccagta ccccctgtcc tgccagcgga
     5281 atcgatgcgt agttgcgcac cggaccgacc gcgctgagac ccggaccgac attgctgagc
     5341 gcggtgatca cggatgttgt ggctgtctcg aggtccgggg tgaagaaggt catcaaaaac
     5401 gagcccagcg caaacaccgc aaaatagata atcacgaagg ctgacacatg cagcaccaga
     5461 tccggctcca ccggtttcgg gccgagtttc acttggatcg ctgctgtcgg gcgcatgaaa
     5521 agccggatct cgcgcaggat tttcttgaag acgatcatga tccggaccac cttgatcccc
     5581 ccgcccgtgg agcctgcgca ggcacccatg agcatcatga acaccagcaa cagacgcgat
     5641 gcgcccggcc agcggtcgaa gtcaaccgtg caaaacccgg tcgaggtcag aaccgaagtg
     5701 ccggtaaaaa aggcgtcgcg caaacaccgt gcgaaggagc cgtgtcccgt ggcccagatg
     5761 ttggcggtca taaaaaggca gcaccccgtc agaaacagaa aataggcacg gcactccgga
     5821 tcgcggacat agtggcccgg ccgcccggtg agcgcggaaa gatgcagcga aaaattgatg
     5881 cccgccagaa acatgaagag cgtgataatc ccttccaccg tcgcgctgtc ataggccgcc
     5941 acactggcgg tacgcgtcga aaacccgccg ctcgcgacag tcgcgaacgt gtggcagacg
     6001 gaatcgaacc atcccatgcc cccgaccccc ttcagcaaca gcgtctgcac gaccgtcagc
     6061 aggagataca cggcccacag cagtttggcc gtggtggcga tgtgcggtgt cagccggtct
     6121 ttgaagggcc cgggcatctc ggctcggtac agttgcatgc cgcccacgcc gagaaagggc
     6181 aggatcgcca cacagagcac cagcaccccc ataccgccaa gccattgcgt cagcgcgcgc
     6241 cagaacagga tcccgcgcgg caggttctca agattgtcca gcactgacgc gccggtggtg
     6301 gtaaagcccg agaccgtctc gaacagcgcc gagaccggat gcgtgatcac gcccgtaagc
     6361 agatacggca acgcgccgaa caaggccgcc gacaaccagc cgagggtcac cacaccgaac
     6421 ccgtcccggc gtcccagatt gagatcgccg cgcgtcaaca tccctgccac cagcgaaacg
     6481 gctagggtga tccctccagc cagaagaaac gcgcaccgca cctccagcgc gtcaccacat
     6541 acgtgggaga ccgcggcaca ggcggtgacc gccaacccga tgaacagggt catgtaggaa
     6601 accagatgga ataccgctcg tttgttcata cgcgcgcgat catttgcgga acatcgcctg
     6661 aatcttcttc accgccgccg ggtcggcgaa aatcaacagg cggtctccag cctcgaaaca
     6721 cagcccaccg gtcgcgggga gcacgccgtc gccgcgctgg accaccgcca cgatcgaccc
     6781 cgacgggaac cggatgtcac tgaccctctt gcccgcatac cgactggtgg gggacaactc
     6841 gatttccagc agttccccgg ggagatcatg cagcagtgcc gccgcgcgca catcccggct
     6901 tctcaggtaa tgcagaatgg cattcgtgat cgactcgtag gggctgatga tccggtccac
     6961 cagcccgagg ttttccatga tcggaatgta gtcggtgcgg gcgatctgag tgaccacgaa
     7021 atccgctccc tttttgcggg ccatcagaca gttcatcgcg ttgtcctcat catcaccggt
     7081 cacggccacg aaagcggtcc cgggaacgat gcccgtctca tccagtgccg agccggacaa
     7141 cgcatcggcc cgcaacacga gcgtatggtc caactcgccg gaacagaaga gcgcccgctc
     7201 ctcatcctgc tccagcagca cgacatgaag accaatgcgc tccaaattct tggcgatggc
     7261 caggccgata tcccccccac ccgccacaat cactttctca aacgcctccc gaaccggatc
     7321 catccaggtt gagaataccc gaatgtcatc cggtgcgccg accagataga gcacatcctc
     7381 cggcagaaat gcggtatccc cccggggaat gaccagacgg ccctgacgcc ggatagcgat
     7441 gatacgcagc ttgcgcacca agtcctgatc cggcaacgta gcgggcgtgt gcccgagaat
     7501 cgggcttccg ccgacgatgc cgaaaccggc caccaggaca cggccgccga acaggtcaaa
     7561 ggactccacc gccccaggca gcaacagggc attacagatt tcctttgcgc aggcgaattt
     7621 ctggttgatc agcagatcga tccccagttt cgtcaggttg tacttgactg caccgggatc
     7681 atactgcgcg ctccccaccc gcgcgatctt gcgcggcacc ccttccgaat gggctaacaa
     7741 acacgcgatg atattgatct catcccgatc cgtcaccgcc acaaacaggt cggccctccc
     7801 ggccccggcc ttttcgagaa tcgcgggatc cgccccggac ccgcagacgg tgagcacatc
     7861 cagcgaggct tcggcctgct ccagcgcctc agccgactcg tcgaccatca ccacactatg
     7921 acgctcccga cagagatgag acgccaacaa ccgccccgca tgcccggcac cgatgattac
     7981 gctattcatg ttgccccttt acacaccctg tatattacga gatgcggcgc gttacacaaa
     8041 cccaaaaaca caatcccccc tttaccgctc accgactggc caccgcgccc ggcatgctgc
     8101 cacacgctgc ccggtaatgg gcggcgacct cttcggctgt caatgactat atcagatcgc
     8161 ggaccttttt ccaaggagac tcgccggatg acgaaggcaa tgcatttccc gtctcccatc
     8221 tcccgtcccc attgttttga ctggcgcgac atgtcataat catgggcacc atagcccaaa
     8281 gaaaggaaca gtcatgaaca agacctccgg aacgggatgg cgtgcggttc tgtgtgcgtt
     8341 gtgtacggtg tggggtgcgg gtgtcgcgga tgcgcagcac gtgaccgggc agaccaaacc
     8401 ggttttgcct gtggtggatc tgtccggaga gacggcgcgg catgtggtga tcgcggcagg
     8461 aacgccggag gagtatcagg gacatccgac cacggtcctc ttaccggacg gcaagacggt
     8521 gttctgcgtg tggtgcatca atcacggcgg cgcggcaggc cccatggcgc gcagtgccga
     8581 tggcggaaaa acatgggtgc ggctggacac cacgctgcca ccgggtttcc ggacgcatca
     8641 gaactgtccc agcatctacc ggctgatcga cccgcagggc aaggcgcggc tctgggtgtg
     8701 gtcagcggcc aaagacacgc ggcagggcac gccgatgccc agcatcatga gcgaggatga
     8761 cggggagatg tggcgggaga tgccgccgct gggcgaagct ttccgctgtg tgatgacctt
     8821 cagcagtatg gttagattga aggacggcag ttatctgggg ctataccaca ggggtgaggg
     8881 caagggcgaa gggcgcaagt tccttgaggt cgtgcaaagc gtgacccgag acggcggatt
     8941 cacttggagc gtgccgcgcg ttgtggccaa tgttgcaggg aagatggcgt gcgagccctt
     9001 cgtgtttcgc tcgccggacg ggagcgaact gtgctgtctg atgcgggaaa acacgcataa
     9061 ggggcacagt ttgcagatgt tcagccgcga tgagggcgcg acttggagcg tgccggaaga
     9121 tacgccgtgg ggattgaccg gtgaccggca catgggcgtg tacgctccgg acgggcgact
     9181 ggtgatcgcc ttccgcgacc aggcacccga atcaccgaca aaaggccatt ttgtggcttg
     9241 ggtcggaaac tacggggaca tccggcagag gcgggacggc cagtacagga tcaagctctt
     9301 gcacgggtat aaacgggttg acctcggtta tcccggcgtg gaacggctgg ctgacggcac
     9361 gcttctggcg accacctatc tcaagtactg ggaggatacg cgccaacact ccgtggtctg
     9421 cacgcgtttc acgctggcgg agacggacgc gaaagcgggc ttgaacccct gagtacggca
     9481 gggatcgcgt tgtgctgtgc ggatgccgga gcggatcacc tgatccgaat ctgcgtgccg
     9541 ggcggcgcgt agtaaagctc cagatggtcg gggaaccttc ccacatgcca cccgtccgga
     9601 agccctgtga cggatgcgaa tgaaccggag agcgttccgg tccatgtcgc cagcgtgtag
     9661 gtctggccgc ggaccagcga atccggctgg agggtgaggg tcaccccctc gccgatcgtc
     9721 agattaccgg tcacggccaa cgtgccgctg ccggaagcgc ctgccgccac caccaatgcg
     9781 ttggtcagcg tggtgtcgcc catgatcatc agtgtaccgt ccaacaccgt atggggagcc
     9841 gtgttctgca cggatccggt gaggatcacc ttcccggggc cggtcttgat gattccgccg
     9901 ggtccggtga tcgcacccga gaaggtcacg ccgctgtcat ccgtgttggc cgcgtgcagg
     9961 cacacgtcgc gcgtcagggc gaggtcgccg gtgaaggctg acgcgccggt tgtctgattg
    10021 ccacccagcg tcatgagcgc gcccggcgcg tagtccatca ggatgatgcc gttccccacg
    10081 gtgtacgggc cgtccgtgag cagggacagc gcgtcgcccg gtgcggttcc ggcgtcgctc
    10141 aggatcaccc gttccgtgcc cagcgcgccc accgtgccat tgccgggcga gtccgcgccg
    10201 accaggattg tgcctttgcg gagggtgacg ctggtggtca tggcgtttgt ggtaccggag
    10261 atacgccagg tgttgttccc ggccttgaca aagcggaagg ccccgcggtt ggaagagccc
    10321 caggttcccc agagataccc ttcgaattcg aagaccccgt tgtcatcgca accgatcagg
    10381 gccacattgt cggaatcgct gttggaaatg ccgcggccgc caaaggcgcc gctcccggtc
    10441 acccgtccga aatggagcgg ataatgcgaa gggtagcaag aggtgaaact gtacacgcga
    10501 tgctccggca caaccagttc gccgttcggc aggccgccgc cgtacgtggc gtcggccgcc
    10561 atcaggcccg acgcgtcgaa gcgcccggtg aacgcatggt tgagcaggtt gtagaaacgg
    10621 aaggaagcgc cggtggccga agagttggtg atcgtcagcg tgcccggccc ctccagccaa
    10681 tccgaggtgc gggttaaccc gtcaaagagg ctccagccca tgttgacggt gatgccgttg
    10741 agatcaacga tcgcaccgcc cgcatcgata tggaggactt tgcggatggc gacatcctcg
    10801 gggttgccgc ctccgttacc cccgccattg ttgccgtaca ggaacccgcc gcctttgagc
    10861 accgcgttgt tgcggacggt catggacatc gacccctcac ccaagaacga gttggcggag
    10921 gtcgttcccg cacggtgctc gatcgagccc tcctccacat caaaaaacat cgtgtactcc
    10981 cgcgcggtga cggtatcttc cgcccggtcc gcgccagagc cgaagccccg gccgacaaac
    11041 catgttcccg cgccgcgctt gaccaggcgt cctccgttcc gcaaaacagg agtggggttc
    11101 gcgtcaagat tggcgacccg gtcccccgcg ccgctgacgt tgagcacgcc gcccgcgctg
    11161 ttgatgtcaa gcgtggtgat attcgtatag ccggggtaca cctcacgggc gaaatcgaaa
    11221 ctgccggtcg tggtcagcgt gccgccatta taaatcatga cgaagttctg cacactcttc
    11281 caggagccgt gccaagagcc gacacccaga cactcggggc cgctgatctc cagttcgcct
    11341 tcaagaaccg aggtgtaatc atcatgccgg ttgtcggcgt tgccgaggat gactttgccg
    11401 gggccggcct tcatcacgcc gacatccgaa agggtgatgc cggtgacctg tcccagatcg
    11461 ttggtgttga agaccggaaa acctaggagt tggctgtcga tccggaccac agcctgggtg
    11521 ttgaagttgt aaatgatgag gttaccggaa cccatgcggg cgatcgcgcc gcccgtgata
    11581 tggaccgggg tgttccccat cgcttcggtc accaggatcg cgccgctctt gatctgattg
    11641 gtgccggaca gattgaggat aatcggattc ccgttgttgg gggtgttgaa gcgcagggtg
    11701 gcgacttcca gattcgcggg aacgctttgc gccagaccgg cggtcacgtc cacatgcttg
    11761 ttcgagccgc tccagtcgga cgcgtagtcc gtgaaattgc tgatggcgtt gccgctcgcg
    11821 gtccgcacgg cccaatccct gcccttaaaa aggatgttgg caggcaggat gttgggatag
    11881 gccgagtttt cggacccgtt ggaggtctgg atgagcttgt agggcccctc cccctccaga
    11941 ttcaggacag agtcggtatt gggtgtcaag aagttgaagg ccagcggcat ggcggaatca
    12001 tggataaccc gcgcgctggc ccctttgtag gtggagagcg tccggaccgc ctgatccggc
    12061 gtggcagtct ggccgttccg gaggatcagg cgcgcgttgt tcccgagcgt gatggtcccg
    12121 tccgcgggcg ttttctggcc gcccgcttcg ctgtagtcca gaatcaaatc gccaagcacc
    12181 acggcggtcg ttccggtata tgcgttgtcc tgcttgagga tcagcgcgcc gaggccgctt
    12241 ttggtcaaac cggaggtaaa accggtcagc gcatggccca gggtgaaatc ggcgccgtcc
    12301 gtgaggagct gtaccggttt gctgttggtg gataccagcg tgaaggcgga ggggtcgacg
    12361 ccggtggagg agtcgatgcg gaacgcgccg ccgttgaaga tcagggggcc gctgcccagg
    12421 cttgaggggg tgagcgcggc caggaacccg ctggtcagca gcgtgccgcc gctgtaggtg
    12481 ttattgcccg cgagcaccag attgcgcaaa gagacgtttt ccagtcggca gccgcgtccg
    12541 ccgtccgtga cggctccgtc gatgcgcagg ttcccgcctt cagaccgggg ataccggctg
    12601 acggtgcccg ccacggcggc atcggaaacg agcgtcacgg ggccggacca gaccgaggtc
    12661 acgcccggcg actggcaacc gctggtcagc gcgcccatgc cgttcgcgcc ccgcccgccg
    12721 atcaggatgg ttttctcagg cccgaaatgg agtccgttca catcaacggt gcccaccccg
    12781 ctggtggcga tcagggtgtt gtcgacgccg aactgattcc ccggcacgtt gtagcggaag
    12841 ggatccccgg cccggacaat ccccttttcc acgatcaggt cgccggaaaa ggtgttgttg
    12901 cccttgagca tcagttgcat gtcctctttg tcgaagcgga cccggatgcc gcggcgtccg
    12961 ccctcctcgc ggatatccct gccgagccag accgcgctga gcgggttact ccagtttccg
    13021 gatgcgcggc cgttgtgctg cacctccaga tcggatttga gcaagagagg aatgttgatg
    13081 gcataccgga aattactggt caggaggggc aggcagcgga ttgtggcggg ctcgccttcc
    13141 accccgctgt cgagggtaag atagttagtg aagacaccga accggtaggt gccgtaaacc
    13201 gggtccgggt taccggatgt ccggttttcc cacggaaggg aaacggtgaa cacggaggtc
    13261 aatccggcga cgggcgtgtc ggcgggcgtg acgatatgcc cgagcacggc attggtgaag
    13321 aagttgttaa gcgtgtaatt gcctttgacg tacagcgtgg cgcccgcgcc tccggggatc
    13381 acgccgtcaa cccaggttgc gggagtggaa aaatttccgg tctgctgcac cgtcccgtct
    13441 tccgcccgga tcaagcctcc tgcgaaaaac ccgatcacac acaagacgtg gattaaattg
    13501 gggattgcat atctcatcac atctcctttt ttacctgttc cgcgtaaaat ccaaaaatcc
    13561 aacggctgaa ttttaaaaag tgtggccatt gcgttgtcct ttcgtttgtg tgactcagtt
    13621 cgacaacatg ctgtc
//
LOCUS       DAOSRB010000065        13562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4626_length_13562_cov_6.319168, whole genome shotgun sequence.
ACCESSION   DAOSRB010000065 DAOSRB010000000
VERSION     DAOSRB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13562
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4626_length_13562_cov_6.319168"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1261)
                     /locus_tag="PLZ74_05080"
     CDS             complement(215..1261)
                     /locus_tag="PLZ74_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOR97729.1"
                     /translation="MKRTFTHLALLATLLSSAFVVADTPVDMTRRAEELKSLRWGLFI
                     CWSFSTFSGKEWTPGIKDLAFFRATEVDTDQWARTAKEAEMGYILFLTKHHDGFCLWD
                     TRTTDRKVTKAPLGRDVLAELKKSCDKYGIKLAFYFSEGEWRHPGGLDDPATPGPKKN
                     NQGGRDPEMKKAQLKELLTQYGPIEYIWFDHAVGDGGLSHTETLAFCKSLQPGCFIGF
                     NHGDQTGADIRLGEMGKPGSLDDHSAAAPYMRNAPSKNYRLAEFTYPILPPHKGGARW
                     FYSLPEHDGLCHTPEKLYRDYLGAARYGNLFSINVGPDYSGKLREIDVRTLRQIGAWI
                     RNPPPPAQVVTRID"
     gene            1468..2649
                     /locus_tag="PLZ74_05085"
     CDS             1468..2649
                     /locus_tag="PLZ74_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOR97730.1"
                     /translation="MFNAYSPELRQDLRFRWNRLQENLRETGADALLAATNVNLLYLT
                     GRIFMGYVHLPAEGEPLFFVRRPCGLSGANVFELRKPEQIPDLLREAGIALPRHLLLE
                     GDDLTFNEHARLTACFPDARIGNGSTDLRQCRAVKTPYEIRIMRLTGSRHAAAVDRFA
                     SVYEPGMTDQAWSIEMFRLMLQAGGLGLFRIAGNSMEGFMGTVLAGDNGGAASPYDFA
                     LGGAGLDPSLPVGQSGVTLTEGMSVMVDIAGNFYGYLTDCTRTFAIGRLAPRAVEAHC
                     RSIEIQQAVAAAGVPGARCEDLYALALKMAEDAGFADCFMGLAQKAKFIGHGTGLVIN
                     EQPVLGARSKETLQEGMCIALEPKFVIPGTGAVGVEDTFLVTPAGMENLTPCDPSMRV
                     L"
     gene            2724..4154
                     /locus_tag="PLZ74_05090"
     CDS             2724..4154
                     /locus_tag="PLZ74_05090"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97731.1"
                     /translation="MGRNGFSRRGFLAAGVAWGFPAIIPSRVLGSQAPSKKIQVGVIG
                     CGRIAGGMDIPGLWHNQDLAVPVALADCDIKRLNTTRAKTARLYDGDLPDLKLYQDYR
                     ALLADKTVDAVMICTPDFWHAQQCVEAAFAGKDVYVQKPLAMSVFESRTIVDVMRRTG
                     RVFHIGTQQRSEGKSTFGPQFRKAAEYVRNGRLGRVRRVEIGLPQEPEEPADWPLVQP
                     VPDTLDYDQWLGYTPAELYSELRTHPQGKGAEADFGRPGWMTLRAYSMGMIANWGAHH
                     IDIAHWGLDLEETGPIRIRGRAEFPKRRLWDVHGSLDMRLTYANGTELHIADNSVYPN
                     GVRFIGEKGWIFCGRGSVKTLSNDPGAGGKHGRWRPLEASTKALIEGEVERPLPRNPG
                     NHHRVWLECIRTRQPAPILPESAHRTTTACILAFTAMNLKRGLKWDPVAERFVGDDEA
                     NATLARPERAPYGVRNALRRAGWASG"
     gene            complement(4194..5261)
                     /locus_tag="PLZ74_05095"
     CDS             complement(4194..5261)
                     /locus_tag="PLZ74_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97732.1"
                     /translation="MAEQDQTPQGAIPPKVTPFIKPNPEKPAVTPAATPAVTPAVTPA
                     ATPAITPAGAPRPITVRLKPVVVPGSPQAAVPAAGVPAPSPTPANLRATSRVLITEAM
                     LPDDAATPSNKDEEAPTSATTATVRPLATAASATVRLRPVTPPSLQPQPPPTPGSDPL
                     PSGPKPPSPAQVQASKSKTSRISLDSAIGVAPMTPMGAGKTEPKTIRLKRPVDLVPPA
                     ASGPKSSTTPIRQTSRIPDSALPSVADSAETASPTQKKTLKIKRPSVKPEGGETAAAA
                     GEAFPDGVQLTPISPLDFPPQEESTVLTTVAVVAGFAAALVLLLLVCCLGAHAVGPDA
                     GKNSVASLSGPELPWGGKLGK"
     gene            complement(5298..5852)
                     /gene="frr"
                     /locus_tag="PLZ74_05100"
     CDS             complement(5298..5852)
                     /gene="frr"
                     /locus_tag="PLZ74_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOR97733.1"
                     /translation="MNDVLLDTVDKMDNTVNMLKDQFSGLRTGKASPALVDGVQVQYY
                     GTPTRLRDLASISTPEPRLIVINAYDPTALPEIEKAILAANLGVTPRNDGRVIRVPIP
                     ELNEERRKEICKVAKRQAEEARIAVRNVRREANDLVKALQKDGKITEDERDRGLEDVQ
                     KTTDAHIAKIDGELKNKEAEVMAV"
     gene            complement(5904..6611)
                     /gene="pyrH"
                     /locus_tag="PLZ74_05105"
     CDS             complement(5904..6611)
                     /gene="pyrH"
                     /locus_tag="PLZ74_05105"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOR97734.1"
                     /translation="MLKLSGEVLGNKQTGECIDPDALASMARRVARIVELGVQIGIVL
                     GGGNIFRGLRGEAKGVNRTTGDNMGMLATIINGLAMQNALEQVGLDTRLQTAIQMHQV
                     AEPFIQRKAMRHLEKGRVVIFAGGTGNPYFSTDSAAALRASEIGASLLLKATKVDGIY
                     TADPNLDPNATRYDKVTYEDALLKRLKVMDSAAFALCMDNRIPIVVFDFYDEDALERI
                     VCGETIGTLVGDAAQIG"
     gene            complement(6818..8485)
                     /locus_tag="PLZ74_05110"
     CDS             complement(6818..8485)
                     /locus_tag="PLZ74_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOR97735.1"
                     /translation="MNNNTLSCFTRSPRFSRLHVCVLTHFVLLPLLLSATEYEYGPYT
                     YIVTDNNEAAIIYYDGDNANVSIPSTLGGYPVAVIGDDVFEMCDSLKTVTIPNSVKSI
                     GNGAFCGCDALKSVTIGNSVTNIGDGVFSDCIALPSVIIPNSVKSIGNGAFCGCEILK
                     SVTIGSSVVTIGDGAFSACIALPSVIIPNSVTSIGDEAFSDCTALTSITIGSSVASIG
                     DSAFYGCESLTSVTIGNSVKSIGEYAFEDCIALKTIVMGNSVTNIGDEAFSNCSSLTN
                     ITVSAGNPKYADRGGVLFDRSLTTLIQYPCGRSGSYTIPGDVTSIGAYAFSWCAALTS
                     VMISSSVTNIGDGAFSNCSSLTNITVSAGNPKYADRGGVLFDRSLTTLIQYPCGRSGS
                     YTIPGDVTSIGAYAFSWCAALTSVMISNGVANIGNRAFTQCGSLAVVLFTGNAPTAGN
                     LVFDGTPATLYYFPDTEGWSSSFANRPAVCWNPAFSSTSSASGMFAFTLTGDADANIP
                     VRIEASDSLTAPFWSAVTDTTIPASGTLNFTDADTRPSRFYRLTFPR"
     gene            complement(8531..11815)
                     /locus_tag="PLZ74_05115"
     CDS             complement(8531..11815)
                     /locus_tag="PLZ74_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR97736.1"
                     /translation="MLGIWVKRSLRYYGLRQVLFAATAALTTAILSAALLTGESLRRT
                     LARGVTARLGTIRSVILRDAGPFPATLADRLPDTQAVLLLNGELLTSEGIVCDARAQI
                     LGAGGESADRVDTDRVWMAALNARGRAQMPLSGEAAIRFAKPRVLPAELPLGAARETR
                     MVRRAVRAGPVADEPTRPPEDFALWPGSVPPVNVRVPLEPLAHAAGVPGMANLLLSTL
                     APRDLERALTTVLTPEDAGLTLTPIGDGGLPTTLLKSRAVYLPRALPAVLARAGLRAS
                     EATFHLVDAFEAGERSTPYGFVAGLTPGTGPVPQDLREDEVVLNAWLAEMLGVETNGT
                     LTLRWRRFEAGGRLVEDQRTFRVRAVTGMTEAASVKTRMPVFPGLEGVDRCAAWDVGM
                     PMDEEKLNDAANEAYWDAWRETPKAFLTYETAKRGFGTLFGEAMSVCVSADTDTVSQA
                     LRGLSPVQAGFTIRPVWDEGLRAAQGTTDFRLLFTGMACVLMIAALTLSGLSLALALE
                     TRKHEAALFRAVGWSRSRVAAMLAAEWGVPLVAGTVAGAGLGAGLSRALVWGLGRFWK
                     DAFAGAETVFLFSWPTALSAVGIGLLLTAAVMFRTVTRMTACPPVALWQSADAPPAPA
                     TSVTGRALRENALGAAMAVAALMLMVAARNSGSAAGAFFGAGFLLMFSLLLFFRTACA
                     RLRREEAPVITSPVAAGVVRTLTTPHRSTAVVLLLACGIFLTLGVLSMRQDPAAGCEH
                     TWSGSGGFASIVSSATPLDRERGLALARRVSGSQGVVPLRVREGDEAGCLNMSLPQSP
                     RLIGLDTRAMARARAFEPRDAGGVWSLLDRPLPDGSIPALAADQAMLRYSLKARADPV
                     DGTVYTYTGAGGETWRVRLVGALPVRSGILQGALLVDEHAFMRMFPAEGYRLWLCDYA
                     PYLLREAADRHTASSPKKNVVGEQAVARLRHPEPGVTVETVAERLRLLGSMEHAYLDL
                     FLVLGGLGLILGVAGMALTFARGMAERRGELALLNAAGLSRRVVLRLLLAEHGVTVAA
                     GLIAGILPALVAIRPAAQTLHSDFPWRTLTLLLFLLATTALLSIYFSIWVASRRIGPD
                     ALKDEA"
     gene            complement(11845..12750)
                     /locus_tag="PLZ74_05120"
     CDS             complement(11845..12750)
                     /locus_tag="PLZ74_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOR97737.1"
                     /translation="MMDWRMRAGVVWIAACACAVAAGLDEVVAPGTVAEPVATGFVFT
                     EGATVNAAGEIFFTDQPNNRILRWSREQGVRVFLSPAGRANGMCFRADGTLLVCADEK
                     NELWSVTPDGAVTVLTSTFEGKKLNGPNDVWARPQGGCYFTDPFYKRRWWTHAEAPQA
                     TQQVYFLPPNGAPIRVTEDLVTPNGLVGTADGTTLYVADLGAERTYAYTILPDGRLDR
                     KRLFCQMGSDGMTLDADGRLYLTHKTGVSVFSPAGTLLGVIPIPESWTANVCIGGPKK
                     DTLFVTAMKTLYAVPLRVRGTPQGK"
     gene            complement(12910..>13562)
                     /locus_tag="PLZ74_05125"
     CDS             complement(12910..>13562)
                     /locus_tag="PLZ74_05125"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOR97738.1"
                     /translation="IADEWEDPNGVPVSAFLFGGRRPTVIPLVNQAKDWTHAVFMGSA
                     AGSETTAANLAKVGVVRRDPMAMLPFFGYNAADYLLHWLTMPLRTDVEKLPKVFFTNW
                     FRKDAKGFMWNGFGDNIRVLKWVCERIDGKGKARETPIGFLPTPDAIDMTGYVDANRD
                     QACMKATMEALLSVDVEGWKKEIAAVAKSYEPFGNRMPFALKAKLAEIQNKLNAAK"
BASE COUNT         2592 a         4144 c         4348 g         2478 t
ORIGIN      
        1 cccgccgagg gtgttgcggc cgtgccgggc aaagcctgag accgcacgtg attggatcac
       61 acatccgacc gcccggaccg gaaacggaaa cgcaaccggt tcgggcggcg ggcatagacc
      121 gtggttcccg gcagtccgtt gccgagaaaa gcgctgtggt cgcatttggc ggacagcacc
      181 ttcccagccc gatcttgtga cccggcaagg cgggtcagtc gatccgcgtg acgacctggg
      241 ccggaggcgg cgggttgcgg atccatgctc cgatctggcg cagggtccgc acatcgatct
      301 cgcgcagctt tccggagtaa tccggcccga cgttgatgga aaagagatta ccgtatcgtg
      361 ccgcgccgag atagtcccgg tagagcttct cgggcgtgtg gcaaaggccg tcgtgttcgg
      421 gcagcgagta gaaccacctt gcgccgcctt tgtgtggagg cagaatcggg taggtgaact
      481 ccgcgagccg gtagtttttg gaaggggcgt tacgcatgta cggggcggct gcagagtgat
      541 cgtccagcga ccctggcttg cccatttctc caagacggat atctgctccc gtctggtcgc
      601 catggttaaa cccgataaag caaccgggct gaagcgactt acagaaggcg agggtctccg
      661 tgtgactcag gccaccatcg ccgacggcgt ggtcgaacca gatgtactcg atgggaccgt
      721 actgcgtcag cagttccttg agttgcgctt ttttcatctc cggatcgcga ccgccctgat
      781 tgtttttctt gggaccgggc gtggcgggat catccagacc gccgggatgg cgccactcgc
      841 cttcggagaa atagaacgcg agcttgatgc cgtatttgtc gcaggatttt ttgagttccg
      901 ccagcacatc gcgtccgagc ggcgccttcg tgactttgcg atccgtggtc cgcgtgtccc
      961 acaggcagaa gccgtcgtgg tgcttggtga gaaagaggat gtagcccatc tccgcctctt
     1021 tcgccgtgcg cgcccactgg tcggtgtcca cctcggtcgc gcggaaaaac gcgaggtcct
     1081 taatacccgg cgtccactcc ttgccggaga aggtgctgaa tgaccagcaa atgaacaggc
     1141 cccagcggag gcttttgagt tcttcggcac gcctggtcat atcgaccggc gtatcggcca
     1201 cgacaaatgc tgaagacaga agtgtggcca gtagtgcgag atgtgtgaat gttcttttca
     1261 ccgttctccc ctcttatccc aaaacggccc taggctgctg cgcgcctcga ccgccacggg
     1321 tgtttacttg cactactgaa aggatacacc cttctgctga aaaccgtcca gcagaaacga
     1381 cgcgggtttc ggggctctct cctgtcttct ctcccctccc tgtgtttgcg ttttactcac
     1441 cgggtctgat ccgctatatt ttttcgtatg tttaacgctt actctcccga actcaggcag
     1501 gacctccgtt tccgttggaa ccggttgcag gaaaatctgc gcgaaaccgg ggcggatgcc
     1561 ctgctggccg ctacaaacgt caacctgctc tacctcacgg gccggatatt catggggtat
     1621 gtccacctcc ccgccgaagg cgagccgttg ttcttcgtgc gtcgcccctg cggactttcc
     1681 ggcgcgaacg tttttgaact ccgtaagccg gaacaaatcc cggacctgtt gcgcgaggcg
     1741 gggatcgccc tgccgcggca cctgctgctg gagggcgacg acctgacatt caacgaacac
     1801 gcgcgcctga ccgcctgctt cccggacgcg cgcatcggca acggttcaac ggatttacgc
     1861 caatgccgcg ccgtcaaaac gccgtacgag atccgaatca tgaggttgac cggcagccgc
     1921 cacgccgcgg ccgtggaccg gttcgcttcg gtatacgaac ccggcatgac cgatcaggcg
     1981 tggtcgatcg agatgttccg gctgatgctt caggccggag gcctgggact gttccgcatc
     2041 gcgggcaaca gcatggaagg gttcatggga accgtgttgg caggcgacaa cggcggtgcg
     2101 gcctcgccct atgatttcgc gctggggggc gcggggctgg acccctcgct tcccgtcgga
     2161 cagagcggag tgacgttgac cgaggggatg tccgtgatgg tggacatcgc cggcaatttc
     2221 tacggatacc tcacggattg cacgcgcacc ttcgcgatcg gccgtctcgc gccgcgcgcg
     2281 gtggaggcgc attgccgttc gatcgagatc cagcaggcgg tggctgcggc gggggtgccg
     2341 ggtgcgcggt gcgaagacct ctacgccctc gcgctcaaaa tggccgaaga cgccgggttc
     2401 gccgactgtt tcatggggct tgcgcagaaa gcgaagttca tcggacatgg cacggggctt
     2461 gtgatcaacg agcagccggt tctcggcgcg cgttcgaaag agacgcttca ggagggcatg
     2521 tgcatcgcgc tggaacctaa attcgtgatc cccggcaccg gcgcggtcgg tgtggaggac
     2581 acgtttctcg tcacgcccgc cggtatggag aacctcaccc cgtgcgaccc ctcgatgcgg
     2641 gttttatgag gttgccgtag gcggcggtgc agtgatatca tgccacgtaa aagtaagtgc
     2701 cggaattcgt gggggaaaga ggcatggggc gaaacggatt ttcgagaaga ggttttcttg
     2761 cagcgggggt ggcatggggg tttcctgcca ttattccgtc gcgcgtgctg gggagtcagg
     2821 cgccgtcgaa aaagatacag gtgggggtga tcggctgcgg ccgcatcgcg ggcgggatgg
     2881 acattcccgg actgtggcac aatcaagacc tcgccgttcc ggtcgcgctg gcggactgcg
     2941 atatcaaacg tctgaacacc acccgcgcca agactgcgcg actctatgac ggagacctgc
     3001 cggatctcaa gctttatcag gattaccggg cgttgctggc ggacaagacg gtcgatgcgg
     3061 tgatgatctg cacgccggat ttctggcacg cgcaacagtg cgtggaggcg gcctttgcgg
     3121 gcaaagacgt ctacgtgcag aaaccgctgg ccatgagcgt gttcgagagc cgcaccattg
     3181 ttgatgtgat gcggcgcacg ggacgcgtct tccacatcgg tacgcagcaa cgctccgaag
     3241 ggaagagcac cttcgggccg cagttccgta aagccgcgga atatgtgcgc aacggccgtc
     3301 tgggacgtgt caggcgggtc gagatcggtt tgccgcagga accggaggaa ccggccgact
     3361 ggccgctcgt gcagccggtg ccggatacgc tggattacga tcaatggctg gggtacacgc
     3421 ccgctgaact ctattccgaa ctgcgcaccc atccccaggg caaaggcgcg gaggcggact
     3481 tcggcaggcc gggctggatg actctccgcg cgtacagcat ggggatgatc gccaactggg
     3541 gcgcgcacca tatcgatatc gcccattggg ggctggatct ggaggaaacc ggaccgatcc
     3601 gcatccgagg ccgggcagaa ttcccgaaac gtcggctctg ggatgtccac ggctcactcg
     3661 acatgcgtct cacgtatgcg aacggaacag agctgcacat cgcggacaac agcgtctatc
     3721 ccaacggtgt gcgcttcatc ggcgagaagg gttggatttt ctgcggccgc ggctcagtca
     3781 agactctgtc gaacgatccg ggtgcgggcg ggaagcacgg acggtggcgt ccgcttgaag
     3841 cgagcacgaa ggccctgatc gagggcgagg tggaacgtcc actgccgcgc aatccgggca
     3901 accaccaccg tgtgtggctg gagtgcatcc ggacgcggca accggccccg atcctgcctg
     3961 aatcggcgca ccgcaccacc acggcctgca ttctggcctt cacggccatg aacctcaaac
     4021 gcggattgaa gtgggatccg gttgccgaac gttttgtcgg cgacgatgag gccaatgcga
     4081 ccctcgcgcg tccggagcgc gcgccctacg gagtacgcaa cgccctgcgt cgtgctggat
     4141 gggccagcgg ttgaatgcgc gaagcagggg aaaagacgtt ttccccgttt cgcttacttg
     4201 cccaattttc cgccccacgg cagctcgggg ccgctgaggg aggccaccga gttcttgccg
     4261 gcatcggggc ctaccgcgtg cgcgccgagg cagcagacca gaagcagcag caccagggcc
     4321 gcggcaaacc ccgccaccac agcgacggtc gtgagtaccg tggactcctc ttggggcggg
     4381 aagtccagcg ggctgatcgg cgtcaactgc acgccatccg ggaaagcctc gccagccgca
     4441 gcagcggttt ccccaccctc cggcttgacg ctgggacgct tgatcttaag cgtcttcttc
     4501 tgcgtcgggg aggccgtttc cgcagaatcc gccacactcg gcagcgcgga atcagggatg
     4561 cgggacgtct gacggatcgg cgtggtagaa ctcttcggtc cggaggccgc aggcggcaca
     4621 agatccacgg gccgtttcaa ccgaatggtt ttcggctcgg tcttgccagc ccccatgggg
     4681 gtcatcggcg caaccccgat ggcggaatcc aaggaaatac gcgaagtttt tgatttggag
     4741 gcctgcacct gcgctgggga cggcggcttg gggccgctcg gcagggggtc cgaccccgga
     4801 gtcggcggcg gttgcggctg caacgacggg ggcgtcaccg ggcgcagacg cacggtggcg
     4861 gaggccgccg tggcaagcgg tcggaccgtg gctgttgtcg cgcttgtggg cgcctcctca
     4921 tccttgttgg agggcgttgc ggcgtcatcg ggcagcatgg cctcggtaat cagcacccgc
     4981 gaggtcgcgc gcagattggc aggtgtaggt gacggggcgg gaacaccggc tgcgggcacg
     5041 gccgcctgcg gggaacctgg gacaaccacc ggcttgaggc gcacggtaat cggacgcggc
     5101 gcacccgcag gggtaatcgc gggcgtggcc gcgggggtaa ccgcgggggt aaccgcgggc
     5161 gtggccgcgg gggtaaccgc aggtttttcc ggattcggct tgataaacgg ggtcactttc
     5221 gggggtatcg caccttgggg tgtttgatcc tgctcagcca taactcctgc ctccgtttgg
     5281 gttcagcgac agccgtatca cacggccatc acctccgctt ccttgttttt caattcaccg
     5341 tcgattttcg cgatatgagc gtcggtggtt ttctgcacat cctccagacc acgatcacgc
     5401 tcgtcctctg taatcttgcc gtctttctgg agcgccttga ccaggtcatt ggcctcgcgc
     5461 cgcacgttgc gcacggcgat ccgcgcctct tccgcctgac gtttggcgac tttgcagatc
     5521 tctttgcggc gctcctcgtt gagttccgga atcggcacgc ggatcacccg gccgtcattg
     5581 cgcggcgtca cgccgaggtt cgccgccaag atggccttct cgatttcagg cagggccgtg
     5641 ggatcgtacg cgttgatcac gatcagacgc ggttcgggcg tggagatgct cgccagatcg
     5701 cgcaaccgcg tcggcgtacc gtaatactgc acctgcacgc cgtccaccaa cgcgggagag
     5761 gctttgcccg tgcgcagtcc cgagaactgg tccttgagca tgttgaccgt gttatccatc
     5821 ttgtcgaccg tgtcgagcaa cacatcattc agcgtatcca tgacattctc ctgtccggcc
     5881 cgccgttaac gggccgcttc cgttcagccg atctgcgccg catcgccgac cagtgtgccg
     5941 atggtctcgc cgcacacgat ccgctccaac gcatcctcat cgtaaaaatc aaacaccaca
     6001 atcggaatcc ggttgtccat gcacaacgcg aaggcggccg aatccatcac cttcaaccgc
     6061 ttgagcagcg cgtcctcata agtgactttg tcgtaccgtg tcgcgttcgg gtccagattc
     6121 ggatccgccg tgtaaatccc atccaccttc gtggctttca gcagcagcga cgcgccgatc
     6181 tcactcgcgc gcaacgcggc cgccgaatcg gtgctgaaat acgggtttcc ggtgccaccc
     6241 gcgaaaatca ccacccggcc cttttccaaa tgacgcatcg ccttgcgctg gatgaacggc
     6301 tcggctacct ggtgcatctg aatcgccgtc tgcagccgcg tatccaaccc cacctgttcc
     6361 aaagcgttct gcatggccag gccgttgatg atcgtggcga gcatgcccat gttgtcgccg
     6421 gtggtccggt tcacaccttt ggcttcgccc cgcaaccctc ggaaaatgtt gccgccgccc
     6481 agaacgatcc cgatctgcac gcccaactca acgatccgcg ccacccggcg ggccatggac
     6541 gccaacgcat cgggatcgat gcattcgccc gtttgcttgt tgcccagcac ctcgccgctc
     6601 agcttcagca aaatccgctt gtactggatt ttaccgctcc gtcgcgtttc cagaaacgcc
     6661 ccctttcagc accgtgatct aaacacagac agattaccga aaccctcctg aaagaacaac
     6721 cgcttttttc gcgtttcgca acccgcaacc caagaagaca gaagagtgga ggagaggagg
     6781 cgggaggcgg gaacggaagg gcaagcattt gactttgtta tctgggaaag gtgaggcggt
     6841 agaaacgcga gggccgggta tcggcgtcag tgaagttgag ggtgccggag gcggggatag
     6901 tcgtgtcggt gacagcggac cagaatgggg cggtgaggct gtcactggcc tcgatgcgga
     6961 cagggatgtt ggcgtcggcg tcgccggtga gagtgaaggc gaacatgccg gaggcggatg
     7021 acgtcgaact gaaggcggga ttccagcaga cggcgggacg gttggcgaag gaggatgacc
     7081 agccttcggt atcgggaaaa tagtagagcg tggcgggagt cccgtcgaaa acgaggttgc
     7141 cggctgtcgg agcgttgccg gtaaagagga cgacagcaag ggaaccgcac tgggtaaagg
     7201 ccctgttacc aatgttggcg acaccgttgc tgatcatgac ggaggtgagg gcggcgcacc
     7261 aggagaaggc gtatgcaccg atgctggtga cgtcaccggg aatggtgtag gaaccggagc
     7321 ggccacaggg atattgaatc agcgtggtta aactacgatc gaacaacacg ccgcctctgt
     7381 cggcatattt cgggttccct gcggatacgg ttatgtttgt aagggaggag caattggaaa
     7441 aggctccgtc gccgatgttg gtgacactgc tgctgatcat gacggaggtg agggcggcgc
     7501 accaggagaa ggcgtatgca ccgatgctgg tgacgtcacc gggaatggtg taggaaccgg
     7561 agcggccaca gggatattga atcagcgtgg ttaaactacg atcgaacaac acgccgcctc
     7621 tgtcggcata tttcgggttc cctgcggata cggttatgtt tgtaagggag gagcaattgg
     7681 aaaaggcttc gtcaccgata ttggtgacgc tattgcccat gacgatggtt ttgagggcga
     7741 tgcagtcttc gaaggcgtat tcgccgatgg atttgacgct gttgccgatc gtgacggatg
     7801 tgagggattc acagccgtag aaggcactgt caccgataga cgcgacactg ctgccaatgg
     7861 tgatggatgt aagagcggta cagtcggaga aggcttcatc accgatggac gtaacgctgt
     7921 tggggatgat aacggagggg agtgcgatac aggcggagaa ggccccgtcg ccgatggtga
     7981 cgacactgct gccgatggtg acggatttga ggatctcgca cccgcaaaag gccccgttgc
     8041 caatggattt gacgctgttg gggatgataa cggaggggag tgcaatgcag tcggagaaga
     8101 ctccatcacc gatgttggtg acgctattac cgatggtcac ggatttgagg gcatcgcacc
     8161 cgcaaaaggc cccgttgcca atggatttga cgctgttggg aatcgtgacg gtcttgaggg
     8221 agtcgcacat ttcgaagaca tcgtcaccga taacggcgac gggataaccg ccgagggtgg
     8281 aaggaatgga gacgttggca ttgtcgccgt cgtaatagat gatggcggct tcgttattgt
     8341 cggtgacgat gtaggtgtag gggccatatt cgtattcggt ggcggataag aggaggggaa
     8401 ggagaacaaa gtgcgtgagc acgcagacat gaaggcgcga aaaacggggc gagcgtgtaa
     8461 agcaggataa ggtgttatta ttcatgggta acctttcata aaacataatt tgaagacttt
     8521 ccgatttcgg tcacgcctcg tccttgagtg cgtcagggcc gatgcgtcgt gacgcaaccc
     8581 aaatggaaaa gtatatactc aacagggcgg tcgtcgcgag cagaaacagc agaagcgtga
     8641 gtgtccgcca gggaaaatcg ctgtgcagcg tctgggccgc gggccggatc gcgaccagcg
     8701 cgggaaggat tcccgctatc agacccgcgg caaccgttac accgtgttcg gccagcagca
     8761 gacgcaacac cacacgacgg gagagcccgg ccgcgttcaa taaagccagt tcaccgcgcc
     8821 gttccgccat gccccgcgcg aaagtcagcg ccatgcccgc gacgccgaga atgagtccga
     8881 gaccgccgag taccagaaaa agatccagat acgcgtgttc catcgaaccc agtagccgca
     8941 aacgttccgc gacggtctcc accgtgactc cgggttccgg atggcgcagc cgtgccacgg
     9001 cttgctctcc gacaacgttt tttttcggag acgatgcggt atgccggtcg gccgcttcac
     9061 gcagcagata gggagcgtag tcacataacc agagacggta tccttcggcg gggaacatcc
     9121 gcatgaaggc gtgttcgtcc accagcagcg caccctgcag gataccgctg cggaccggca
     9181 atgcgcccac aaggcgcacg cgccaagttt cgcctcctgc ccccgtgtag gtatacaccg
     9241 tgccgtccac cggatcggcc cgggccttca ggctgtaccg gagcatggcc tgatccgccg
     9301 ccagcgcggg gatcgatccg tccggcagcg gacggtcgag gagcgaccag acgccgcccg
     9361 cgtcacgggg ttcgaatgcc cgtgcgcgcg ccatggcccg cgtgtcgaga ccgatcagac
     9421 gcggcgattg gggaagactc atgttcaggc agcccgcctc gtcaccttcc cgcacacgga
     9481 ggggcacaac cccctgactg ccgctgacac gccgcgccaa ggcgaggccg cgttcgcggt
     9541 cgagcggcgt ggcggaggag acgattgacg cgaatccccc gcttccagac caggtgtgtt
     9601 cgcatccggc cgcaggatcc tgacgcatcg agagtacacc gagggtcagg aagatgccgc
     9661 acgccagcag aaggaccacc gccgtgctac ggtgcggcgt cgtcagcgtg cgaaccacgc
     9721 cagccgcaac gggagaggtg atcaccggcg cctcttcccg ccgcaggcgc gcgcaggcgg
     9781 ttcggaagaa cagcaagagc gaaaacatca ggagaaaacc ggctccgaaa aacgcgcctg
     9841 cggcggagcc gctattacgt gcggcgacca tcaacataag cgccgccacg gccatggccg
     9901 cacccaacgc gttctcgcgc agagcgcggc cggttacgga cgtcgcgggt gcgggcggcg
     9961 cgtcagcact ctgccacagc gcgaccggtg ggcaggccgt catccgggtg acggtccgga
    10021 acattactgc ggccgtgagg agcagtccga tcccaaccgc gctcagtgcc gtaggccaag
    10081 agaagagaaa gaccgtctcc gccccggcaa aggcatcctt ccagaaccgg ccaaggcccc
    10141 acaccaaagc gcgggaaagt ccggcaccaa gcccggcgcc cgcaaccgtt cccgcaacaa
    10201 gcggaacgcc ccattcggcg gccagcattg cggccacccg cgaacgactc cagccgaccg
    10261 cgcggaacag ggctgcctcg tgtttgcggg tttcgagcgc caaggccaga gagagtcccg
    10321 agagggtcag agccgcgatc atcaatacgc atgccatgcc ggtaaaaagc aggcggaaat
    10381 cggttgtccc ttgtgccgcc cgcaacccct catcccaaac cggccggatc gtgaatcccg
    10441 cctgaaccgg cgagagcccc cggagcgctt gcgacaccgt atccgtgtcg gcagagacgc
    10501 acacgctcat cgcctcgccg aacagcgtgc cgaagccgcg cttcgccgtt tcgtaggtga
    10561 gaaacgcctt gggggtttca cgccacgcgt cccagtaagc ctcattggcg gcgtcattca
    10621 gtttttcctc atccatgggc atgcccacat cccacgccgc gcaccgatct acgccttcga
    10681 gccccggaaa aaccggcatc cgcgttttga cggacgcggc ttcggtcatg ccggtcaccg
    10741 cacgcacacg gaatgtccgt tgatcctcca caagccgtcc gccggcctca aaacgacgcc
    10801 agcgcagggt cagggtgccg ttcgtctcca cacccagcat ttcggccaac caagcattaa
    10861 gaaccacctc gtcttcgcgc aaatcctgcg gcaccggtcc ggttcccgga gtcaggcccg
    10921 cgacaaaacc gtagggcgtt gaccgttcgc cggcctcgaa agcgtcgaca aggtggaagg
    10981 tcgcttcgct cgcacgcagg cccgcacgcg ccagcacggc aggcagggca cgcggcagat
    11041 acacggcgcg gcttttcagc agtgtggtcg gcaagccccc atcccctatc ggggtgaggg
    11101 tgaggcccgc gtcctctggg gtcaacaccg tggtcaacgc gcgttcgaga tcgcggggcg
    11161 cgagcgttga aagcagaagg ttggccatgc cgggcacgcc tgcggcgtgc gcgagcggtt
    11221 cgagcggcac acgtacgttc acagggggta cggaaccggg ccagagcgca aaatcctcag
    11281 gggggcgcgt gggctcgtcc gccacaggtc ccgcacggac ggcgcgccga accatccggg
    11341 tttcccgtgc cgcgccgagc ggcaattcgg cgggcaagac ccggggtttc gcgaaccgga
    11401 tggccgcttc gcccgaaagc ggcatctgcg cgcggccccg ggcgttgagc gcggccatcc
    11461 agactcggtc cgtgtcgacg cggtccgcag attcgccgcc cgcgccgagg atctgcgcgc
    11521 gtgcgtcgca cacgatcccc tcggaagtca gcagctcgcc attcagtaat aggacggctt
    11581 gcgtatccgg cagacggtca gcgagtgtgg cgggaaaggg tccggcatca cgaagaatga
    11641 ccgaacggat cgttccgaga cgcgcggtca cgccgcgcgc cagtgtgcgc cgcaacgatt
    11701 cgccggtgag cagcgcggcg gacaagatgg cggtggtcag ggccgcggtc gcggcgaaaa
    11761 gcacctgccg caagccgtaa taacgcaggc ttcttttgac ccagatgccc agcatgggag
    11821 tctccgaacc gcgcccctac tttctcactt gccctgcggc gtgccccgaa cccgcagcgg
    11881 gaccgcgtac agggttttca tcgccgtcac aaaaagcgtg tcttttttcg ggccgccgat
    11941 gcagacgttg gcggtccagg attccggaat cggaatcacg cccagcaggg ttccggcggg
    12001 actgaagacg ctgacgcccg ttttatgggt cagataaagc cgaccgtcgg catccagggt
    12061 catgccgtct gaacccatct ggcaaaagag gcgtttgcgg tcgagacgcc cgtccggcag
    12121 gatggtatag gcatacgtcc gttcagcgcc gaggtccgcc acataaagcg tggtgccgtc
    12181 ggccgtgccg accagtccgt tgggggtgac caggtcttcc gtcacacgga tgggcgcgcc
    12241 gttcggcggc agaaagtaaa cctgctgtgt ggcttggggc gcctcggcat gggtccacca
    12301 gcggcgtttg tagaaggggt ccgtgaaata acagccgccc tgcggccgcg cccagacgtc
    12361 attcgggccg ttcagttttt ttccctcaaa ggtgctggtc aggacggtga ccgcaccgtc
    12421 cggcgtgacg gaccacagtt cgttcttctc gtcggcgcag accagcagcg tgccgtccgc
    12481 acggaagcac atgccgttgg cgcggccggc cggactcagg aaaacccgta ccccctgttc
    12541 gcgcgaccag cgcagaatcc ggttgttcgg ctggtccgtg aagaaaatct ctcctgcggc
    12601 attgacggtc gccccctcgg tgaagacgaa gccggttgcc accggttcgg ctaccgttcc
    12661 gggcgccacc acctcatcca gtccggccgc caccgcgcac gcgcaagccg cgatccaaac
    12721 cactcccgcc cgcattctcc aatccatcat cggttacccc cttcccggtt tcccggtttt
    12781 tctgttttcc tgtctgacgg gacgcagtgt acagttttcg gaccggtaac acaaacagcg
    12841 attgttttcg gaaagaatgc aaaacggggc ccgggacatt gccgtcccga gccccgttgg
    12901 atgaaacggt tatttcgccg cgttcagttt gttctggatc tcggccagct tcgccttgag
    12961 cgcgaacggc atccggttgc cgaacggctc atacgatttc gccactgcgg cgatctcttt
    13021 tttccaccct tcgacatcca cagagagcag ggcttccatc gttgctttca tgcaggcctg
    13081 atcccggttc gcgtcaacgt aaccggtcat gtcgatggcg tcgggcgtgg gcaggaaccc
    13141 gatgggggtc tcgcgcgcct tccccttgcc gtcgatccgc tcacaaaccc acttcagcac
    13201 gcggatgttg tccccgaagc cgttccacat gaaccctttc gcatccttgc ggaaccagtt
    13261 cgtgaaaaac accttgggta gcttttccac atcggtgcgc aaaggcatgg tgagccagtg
    13321 cagcagataa tccgccgcat tgtagccgaa gaacggcagc atcgccatgg ggtcacgacg
    13381 aactacgccg accttggcga ggttggcggc ggtggtttcg gaaccggcgg cggaacccat
    13441 gaacaccgca tgcgtccagt ccttggcctg gttcaccagc gggatcacgg tcgggcggcg
    13501 gccgccgaac aggaaggcgg aaaccggcac gccgttggga tcttcccact cgtcggcgat
    13561 ca
//
LOCUS       DAOSRB010000066        13440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4670_length_13440_cov_7.162122, whole genome shotgun sequence.
ACCESSION   DAOSRB010000066 DAOSRB010000000
VERSION     DAOSRB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13440
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4670_length_13440_cov_7.162122"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /gene="rho"
                     /locus_tag="PLZ74_05130"
     CDS             complement(<1..1185)
                     /gene="rho"
                     /locus_tag="PLZ74_05130"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOR97739.1"
                     /translation="MSTEHEASEQNVSQNSGGQSDGAAQAAPSQQAGGGGGKWTPRFT
                     PRPPSSARPSSSGRRDGRSSRGGRGRARPQKHLSRGGSARHSSKSQHLPKGLPADYDQ
                     PDMPPSPAPQNGEGAPDTAHPTETLPPVYLTDLQKQEVAELLELLPNANSEDLAALRK
                     HEIIFEVIRSYLRRGGTVITSGVLEILPDGYGFLRSPKSNYLQCPEDVYVSPSQVRRF
                     GLRTGDVVEGPVRDPREKERFFALGRVDMMNGKPPVEARRTVHFENLTPLFPDRRIQL
                     ETSPDEISMRVMDIFTPIGFGQRGLIVAPPRTGKTVLLQKVANAISANHPDAMLIILL
                     IDERPEEVTDMQRNTKAQVLSSTFDEAPERHVQVAEMVIEVAKRQVENGRDVVILLDS
                     ITR"
     gene            complement(1269..1880)
                     /gene="coaE"
                     /locus_tag="PLZ74_05135"
     CDS             complement(1269..1880)
                     /gene="coaE"
                     /locus_tag="PLZ74_05135"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOR97740.1"
                     /translation="MFKRFAITGGIACGKSLFSRELERLGVEVRDADEVVHALEAPGG
                     EAVAALRKKLGPGIVGADGGIDRRALGERVFRDDETRAVVNAVLHPLVRERLLAWADT
                     PGPRLKVAVIPLLFEAGWERDWDVVICLVCCEAVQRERLMRSRGLTEEEARLRIAAQW
                     PVAEKAARSHYVVYNESDVAALAREADRIVRLLAASRMDHGGT"
     gene            complement(2188..>2535)
                     /locus_tag="PLZ74_05140"
     CDS             complement(2188..>2535)
                     /locus_tag="PLZ74_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97741.1"
                     /translation="SVQTQLSVYNGATVVMGGLITESRKTMEDKIPFLGDLPYVGRLF
                     RSRSEESDKRNLLVFVTARLVDPAGRAVRMSGESALLSGGGGAASPVSQQGAEAAAQP
                     AAAAPQPPQAEQN"
     assembly_gap    2536..2635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2636..>5077)
                     /locus_tag="PLZ74_05145"
     CDS             complement(<2636..>5077)
                     /locus_tag="PLZ74_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97742.1"
                     /translation="PKAEEKPAEPAPAAVAAPKAEEKPAEPAPAAVAAPAAEEKPAAP
                     APAAVAAPKAEEKPAEPAPAAVAAPAADDHASLVSELLTLESIRRKSLDDHGYASLET
                     ARKALKEGDYPLAREQYKQALDFIVSRPATAPALEEARQGMAEAFYQEAKQATRKGDY
                     ERAMPLAVQAQEHGHPQAPRLIETLKKEPQKEAMDVSAISHRVNEDSYKGQRDEIRRR
                     LRLSRQFYTTGEYAKALEECELILRDYPFDTEAMELRDRISDRMKTVADVELEATRSL
                     MIRDVRKSWTPERYAIESAQMPRGKSDMTAKRAPGEISSTKTSEQIIQQKLRDIVIPE
                     VTFRPPATIIDAVDFFKQASRDYDKPEIPVEQRGVNLVLKLAPGATAAQAAPAVEDPF
                     APAAPAGAASGVPAIPALSARFINLFDALKLVCDVTGMKFRIRGNIVMIVPVNDPDDE
                     LVTRSYNVLATLQERVGAAQSELTQSRGGGDATFMQQADMGGKQDWKTFFQQMGVKWP
                     EGSSISYMATIGKLRVTNTAEELAVFEQVLEDLNVTPRLIEIEARFVEVSQKDLNSLG
                     FEWLLNSDFSFDAGGFLNKALSLKEVSNKQVPLLDSVGMPVFDAAGNQLYAYQPYQAI
                     NQTIRTDNIPGSIIGYDGTGQPIPAQNLAAGQYTMMNAGNHNMGINAIDGNTYGIGNR
                     YLSTSGNPIAGEGKSINDQFMRVNAFLGNADLSMILHMLSQRSDTDLLSAPKVVTKSG
                     QEAVMKVVTEYIYPTEYEVQISQQSSSGSITAGGSSDPLAIVEPQNFEMREVGIILQV
                     VPEVSAEGQMINLML"
     assembly_gap    5078..5177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5461..6531)
                     /locus_tag="PLZ74_05150"
     CDS             complement(5461..6531)
                     /locus_tag="PLZ74_05150"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOR97743.1"
                     /translation="MAIKLSSFPLLRHYDKLIAIGVLIFLLISLFYLTHVGAARKQAE
                     SQYTRQLESLKPTAAPLAAIEMAEFDDAVRLVRSPLQLDTAETQKAGFLTPERRVVCV
                     IQACKKPIPYAAEVCPFCGGKQPIPPDMDVGLDTDRDGIPDKEEIRLGLNPLDPADAK
                     GDLDGDGFSNLEEFLAGTDPKDPKSHPALVNLLRVKELRGKRLPLIFSGVNKMPDGKL
                     QLVFNQTAPIPRTFWIREGERIGDSDYVAEQVEIKFEDRDNPRMPGLKMRVDVSRVTL
                     LRQSDKKKIVLQINERDKNTDIEAVIVLPLDQTEYTVIENSTLKVREETYRVLTIDAD
                     TTTVIIESQATGQQKVVRKLDS"
     gene            complement(6535..7512)
                     /locus_tag="PLZ74_05155"
     CDS             complement(6535..7512)
                     /locus_tag="PLZ74_05155"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HOR97744.1"
                     /translation="MKRQQLILIILAVLTVGVCLGAGWFLVSAWMANKEAASVRNADY
                     AAVQKIYRGKVFPDPDTIARVKEDQKALESWLATASNLLHQGDLHIESKSPTGFKQTL
                     QATVRALSARPGAANGRIVAPGFLFGFDQYLGESDSLPAPEHVDRLTGQLTIIERLCN
                     ELCEAGVLEIKAVQRETFDAQTEAGGPDRSQEASSSRRRRRRDEPQAQEAAAPAARKA
                     ASGEFFSKQRFSFEFLARPPAFIGALNRLAAMDLFVVVAEAEFRKTDDPIGRHDTRKK
                     EGAASAADAATKEVDLATIPHAERIVTDPDLDPPVNVRLNIDVYSFEGV"
     gene            complement(7509..9200)
                     /gene="pilM"
                     /locus_tag="PLZ74_05160"
     CDS             complement(7509..9200)
                     /gene="pilM"
                     /locus_tag="PLZ74_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOR97745.1"
                     /translation="MFSTDRILALNVGASKLVLAEFSVKSGRPPELLNYGTHVFGVEP
                     ENESETGAQLVPAIREIMRTHGIRNAPLMLSLSGQMVFPRFVRLPAVGEDKLLQMVQY
                     EVEQNVPFPMEEIVWNHQFIGDASTGEQCAMIVAAKIENVRDITDRVVEAGLDPETVD
                     VAPMTLYNCLRYNYAELDGCSMLLDIGARSTNLIFLEDEKIYNRCIPVAGNAITQELA
                     KSFQISFAEAEALKCERAFVSLGGVSASEDEVSERVSKVVRNVVTRLHAEISRSINFY
                     RSQQGGNAPVRIFLTGGSSVMPHLDTFFREKLQVEVLYLNPFTNVAVSNRIDREKAGV
                     DAFVLAETVGLALRRSLACPVEINLMPPEITKRKTLKRRIPFFGLAAAGVFLSLGIWT
                     LYLKQMGKLYAFQGEQVAAKLSVCKAKQASLTQAEKSREAVREKAEAIRDLMWCRTAW
                     LKRVDAVRKSLFEGMWLTELAPVRDAAGTVTGLRIVGRGWADKHKEIEEKAKSAGRTA
                     TAVEELRDRLKTQSVFSGEPQGVRIVGVKDIGAYLIEFTIEATLAASGPKDEAAR"
     gene            complement(9200..9880)
                     /locus_tag="PLZ74_05165"
     CDS             complement(9200..9880)
                     /locus_tag="PLZ74_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97746.1"
                     /translation="MNKTVVGRTGMSVMGVAVAMLICTEAAMAQMSRGQTRMGQATAV
                     RTEKREEVNITRMPPPNKTAMVRTPEFNVNVQNTMPKVTRRPREWALFEVKYNSSAKW
                     TDELVFTYHVMTKGKDEEGKEGFSYYTLTVRYIDISKGEHMSCVALPPSQVERHGEPV
                     AVALEITNKEGKVMDSKSESTIPLPKEWWKDSKVLDNAALTRRTGLLDRSKTPFALIN
                     ADDYEVVQ"
     gene            10399..10662
                     /gene="rpsO"
                     /locus_tag="PLZ74_05170"
     CDS             10399..10662
                     /gene="rpsO"
                     /locus_tag="PLZ74_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOR97747.1"
                     /translation="MSQIDKTAIRKQFQRHDRDTGSSEVQIAVLTKEIEMLTEHLKAN
                     KKDHSSRYGLIRKVNTRRSLLDYLKREDEAVYQKIIKELGLRR"
     gene            10821..12938
                     /gene="pnp"
                     /locus_tag="PLZ74_05175"
     CDS             10821..12938
                     /gene="pnp"
                     /locus_tag="PLZ74_05175"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOR97748.1"
                     /translation="MKDTTSVSVQVGGATMTIETGLLARQAAGATVCRLGDSLMFSAV
                     TCTDKPREGIDFFPLQVEYREKYYAAGRFPGGFFKRESRPSEKEILTARITDRPIRPL
                     FPEGYRNDVQINNMVLSADTENDTDILSVNASSAALHISDIPFMGPIGCVRVGRIHGE
                     FVINPTHSQRKESDLDLVYAGTRERFLMMEGGAAEITEEDFLAAMKVGHAEVVKIIDA
                     QHELRRLLGKPEKKIEEVAPNAEKMAFLYAEGETALRQALLIVDKLERQNAVNAIKED
                     LQAKALAKWPEEVTAASFVTLFDTLEIDLVRRNILEDGKRIDGRAADEIRKLYAQVGV
                     MPRAHGSAIFERGETSALGSVTLGTKKDAQELDAITGGETSKSFFLHYNFPPYSVGEV
                     GRLGATGRREIGHGALAERSLAQVMPQDYPYSIRVVSDIMGSNGSSSMASICVGSLAL
                     MDAGIPIKAPVAGVSVGLFTNKDESQKILVTDILGAEDHCGDMDFKVAGTRKGITGFQ
                     VDLKLRGLTWDVVEAALARAKQGRLQILDFMESVMPAPRAELSQFAPRITVMTIPVDK
                     IGALIGPGGSNIRRVCEISGTQIDIQDDGSVSIFANNAESLAIAQNEVLALTAEAEEG
                     KIYEGTVTGIKEFGCFVEIMPGKDGLCHISELADRRIGSVEDVCKVGDKMTVKCIGVD
                     DRGRIKLSRREAMRDLDQQKQNG"
     gene            complement(13169..>13440)
                     /gene="fucI"
                     /locus_tag="PLZ74_05180"
     CDS             complement(13169..>13440)
                     /gene="fucI"
                     /locus_tag="PLZ74_05180"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635899.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOR97749.1"
                     /translation="WFVPRLTGKGAFRDVYSVMNNWSANHGAISYGHIGADLTTLAAM
                     LRIPVAMSNVPEEQIFRPHAWGLFGIDDPIGADYRACKNFGPIYG"
BASE COUNT         2488 a         4346 c         3866 g         2540 t
ORIGIN      
        1 acgcgtgatg ctgtccagca ggatcaccac gtcccggccg ttctcaacct ggcgtttggc
       61 cacctcgatg accatttcag cgacctgcac gtgccgttcc ggagcctcgt caaaagtcga
      121 actcaacact tgcgccttgg tgttgcgctg catgtcggtg acctcttcgg gccgctcgtc
      181 gatcagcagg atgatcagca tcgcgtccgg gtggttagcg gagatcgcgt tggccacctt
      241 ttgcagcaac acggtcttgc cggtgcgcgg cggcgccacg atcagcccgc gctgaccgaa
      301 tccgatcggc gtgaaaatat ccatgacgcg catcgaaatc tcgtccggcg aggtttccaa
      361 ctggatgcgc cggtccggaa acagcggcgt cagattctcg aaatgcaccg tacgccgcgc
      421 ctccaccggc ggtttcccgt tcatcatgtc cacgcggccc agcgcaaaga aacgctcctt
      481 ttcccgcggg tcgcgcaccg gcccctccac cacgtcgccg gtccgcaatc cgaagcgcct
      541 cacctgcgaa ggcgagacat agacatcctc cggacactgc aagtaattgc tcttgggcga
      601 acggagaaag ccgtatccat ccggcaggat ttccaacaca ccgctggtga tcaccgtgcc
      661 gccacggcgg agatagctgc ggatgacttc gaaaatgatt tcgtgcttgc gcagggccgc
      721 cagatcctcg gagttggcat tcggcaacag ctccagcaac tcggcaacct cttgcttctg
      781 gaggtcggtg aggtacacgg gcggcagcgt ctccgtcgga tgcgcggtgt caggcgcacc
      841 ttcgccgttc tgcggcgcgg gactaggagg catgtccggc tgatcgtaat ccgcaggcaa
      901 ccccttgggc aagtgttgcg atttcgacga gtggcgggcc gaaccaccgc gcgacaagtg
      961 cttctgcggc cgtgcccggc cccggccccc gcggctacta cggccatccc ggcgcccgct
     1021 cgaagaaggc cgtgcgctag aaggcggacg cggagtgaat cgcggcgtcc atttcccccc
     1081 ccctcctccc gcttgttggg acggcgcggc ctgcgcggcg ccatcggatt ggccacccga
     1141 attctgcgaa acgttctgct cggaagcctc gtgctctgta ctcattggtt ctctcctcat
     1201 gcgggcaaag cccgcaatcc tcacctctgc cctccctata ctaaaggggg aagggcgtca
     1261 acaactcgtt acgtgccgcc atgatccata cgtgaggcag cgagcaggcg gacgatccgg
     1321 tccgcttccc gcgccaacgc tgcaacatcg gactcattgt aaaccacgta gtgcgaccgg
     1381 gcggctttct cggctaccgg ccactgcgca gcgatgcgca ggcgggcctc ctcttccgtc
     1441 agtccccgag agcgcatcaa ccgttctctc tgcaccgcct cacagcagac gaggcagatc
     1501 acgacatccc aatccctctc ccaccccgcc tcgaaaagga gcgggatcac cgcgaccttc
     1561 aggcgtggcc ccggcgtgtc ggcccaagcg agcaaccgtt cccggaccag cggatgcaaa
     1621 accgcattca cgaccgcacg cgtctcatca tcccgaaaca cgcgttcacc cagcgcacgg
     1681 cggtcgatcc cgccatccgc tcccacaatt cccggtccca gcttttttcg taacgccgca
     1741 accgcctccc cgcccggagc ctccagtgcg tgaaccacct cgtcggcgtc acggacctcc
     1801 acccccaaac gttccagttc ccgggaaaag aggcttttac cgcaggcgat cccgccggtt
     1861 atggcaaacc gcttgaacat acgatgacac acgcgaagcg cgtcagaaac cggacgggac
     1921 ggagcccccc ccggaacagg acaaaaaccg ctgaacgtca tccgcccgtt cccgttcccg
     1981 ttccgcgggc ggcactctct cccgtgacgc ctacggtggg gacgcctcac cgaagtccgt
     2041 tgtccacttc gggttcgggg gaaccgcctt accctgctgc gcggttcttt catctgacgc
     2101 cgacagagag tgacgcctcc cctgtccgca cgcgcactcg cgcaggcagg ccggggagag
     2161 tggcggcacg cgccgcggcg ggattactta attctgctcc gcctgcggcg gctggggcgc
     2221 cgcggcggcc ggctgcgccg ccgcctccgc accctgctgc gacaccggag acgccgcgcc
     2281 gccaccgccc gaaagcaggg ccgactcgcc gctcatgcgc accgcgcgac ccgcgggatc
     2341 cacaagccgg gccgtcacaa acaccagcag attccgcttg tccgactctt ccgaacggct
     2401 gcggaaaagg cggccgacat acggcaggtc gcccaggaac ggaatcttgt cctccatcgt
     2461 cttgcgggac tcggtgatca gcccgcccat caccaccgtg gcgccgttgt aaaccgaaag
     2521 ctgcgtctgc accgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgag
     2641 catcaggttg atcatctggc cctcggccga gacctccggc accacctgca ggatgatgcc
     2701 cacctcgcgc atctcgaagt tctgcggctc cacgatcgcc agcgggtccg acgaaccgcc
     2761 cgccgtgatg ctgcccgaag agctctgctg cgaaatctga acctcgtatt cggtcggata
     2821 gatgtactcg gtcaccacct tcatcaccgc ctcctggccg gacttggtga ccaccttggg
     2881 ggccgagagc agatccgtgt ccgaacgctg gctcagcata tggagaatca tcgagaggtc
     2941 cgcgttgccc aggaaggcgt tgacccgcat gaactggtcg ttgatgctct tgccctcgcc
     3001 cgcgatcggg ttgccgctcg tgctcagata acggttgccg atcccgtacg tgttgccgtc
     3061 gatcgcgttg atccccatgt tgtggttgcc ggcattcatc atcgtgtact gtccggcggc
     3121 aagattctgc gccgggatcg gctgcccggt tccatcatac ccgataatcg atcccggaat
     3181 gttgtcggtg cggatcgtct ggttgatcgc ctgatacggc tgatacgcgt aaagctggtt
     3241 gcccgcggcg tcaaacacgg gcatccccac tgaatccagc agcggcacct gcttgttgct
     3301 gacctctttg aggctgaggg ctttgttgag gaatccgccc gcgtcgaagc tgaagtcgct
     3361 gttgagcagc cactcgaacc cgagggagtt gaggtccttc tgggagactt cgacaaagcg
     3421 cgcttcaatc tcaatcaggc gcggcgtgac gttgagatcc tccagcacct gctcgaacac
     3481 cgcgagttcc tcggcggtat tggtgacgcg cagcttacca atggtagcca tgtaggagat
     3541 tgaggagccc tcgggccatt tgacgcccat ctgctggaag aaggtcttcc agtcctgctt
     3601 gccccccata tcggcctgct gcatgaaggt ggcgtctccg ccgccgcggc tctgggtcaa
     3661 ctcgctctgg gccgcgccga cgcgttcctg cagcgtggcc agcacgttgt aggagcgggt
     3721 gaccaactcg tcatccggat cgttgaccgg cacgatcatc acgatgttgc cgcgaatacg
     3781 gaatttcatg cccgtgacat cgcagaccag tttcagcgcg tcaaaaagat tgatgaaccg
     3841 cgcactcaac gcggggatcg caggcacccc actcgccgcg cctgcgggag cggcgggcgc
     3901 gaagggatcc tccaccgcag gggcagcctg cgctgccgtg gcgcccgggg ccagcttaag
     3961 caccaagttg accccgcgct gctcgaccgg aatctccggc ttatcgtagt cgcgactcgc
     4021 ctgcttgaag aaatcgaccg catcaatgat cgtcgcggga ggacggaagg tgacttcggg
     4081 gatcacgata tcacgcagtt tctgctggat gatctgctcc gacgttttgg tgctgctgat
     4141 ctcgcccggc gcacgtttgg cggtcatgtc gctcttgccg cgaggcatct gggcgctttc
     4201 gatcgcgtag cgctcgggcg tccaactctt gcgcacatcc cggatcatca gactgcgggt
     4261 cgcctccagt tcgacatcgg cgaccgtctt catacggtca gagatccggt cgcgcagctc
     4321 catcgcctca gtatcaaaag gataatcgcg cagaatcagc tcgcactcct ctaaagcctt
     4381 ggcatactcg ccggtggtgt aaaactgacg tgacaaccgg agacggcggc gaatctcatc
     4441 acgctgaccc ttataggaat cctcattcac gcggtgcgag atagccgaga cgtccatggc
     4501 ctctttctgc ggctccttct tcagcgtttc gatcagccga ggcgcctggg ggtggccatg
     4561 ttcctgcgcc tgaacagcca gcggcatagc ccgctcgtaa tcgcccttcc gggtcgcctg
     4621 cttcgcttct tggtagaatg cctcggccat cccctgccgc gcctcctcca gcgccggagc
     4681 ggtcgccggg cgactgacga taaaatccaa cgcctgctta tactgttcgc gggccaacgg
     4741 gtaatccccc tctttcagtg ccttacgcgc ggtttccaac gaggcgtacc catgatcatc
     4801 caatgacttg cggcggatcg actccagagt gagcagctcg gacaccagac tcgcatgatc
     4861 atccgccgcc ggcgcggcca cagcggcggg cgcgggctcg gcgggcttct cctccgcctt
     4921 aggcgcggcc acggcagccg gtgcaggcgc ggcgggcttc tcctccgccg caggcgcggc
     4981 cacggcggcg ggcgcgggct cggcgggctt ctcctccgcc ttaggcgcgg ccacggcggc
     5041 gggcgcgggc tcggcgggct tctcctccgc cttaggcnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnncgg ccacggcggc gggcgcgggc tcggcgggct tctcctccgc
     5221 cttaggcgcg gctacagcgg cggcaggcgc ttttttcttc tgtccgccta aatcctgcag
     5281 gagcgcttcg agatcgtcct gggcatgcag aaccgtcatc gcgcaacagg ccatgactcc
     5341 gatacccgac accaacaacc accttgaact atgcgtcatc tggggggact cctctttgtg
     5401 tggcagctat ccaatcctat ctggtaataa aaatcaggct tactataccg tagacggcgt
     5461 tcacgaatca agcttgcgaa caactttttg ttgtccggtc gcctggctct cgataatgac
     5521 cgtcgttgtg tccgcgtcga tcgtgagcac acggtaagtt tcctcacgga ccttcaacgt
     5581 gctgttctca atcaccgtgt actcggtctg atcaaggggc agaacaatca ccgcctcgat
     5641 atccgtgttt ttgtcacgtt cattgatttg cagcacgatc tttttcttgt cgctctggcg
     5701 gagcaatgtg acacgtgaca catcgacccg cattttgaga cccggcattc gcggattatc
     5761 ccgatcctcg aacttgattt caacctgttc ggccacatag tcactgtcgc cgatacgttc
     5821 gccttcacgg atccagaagg tgcgcgggat cggtgcggtc tggttgaaca ccagctgcag
     5881 ttttccgtcc ggcattttgt tcacgccact gaagatcagc ggcagacgct taccgcgcag
     5941 ttctttgaca cgcaacagat tgaccagcgc cgggtgggat ttcggatctt tgggatccgt
     6001 gccggccaga aactcctcga gattcgaaaa cccgtcgccg tccagatcgc ctttggcgtc
     6061 agcgggatcc agcgggttga gcccgaggcg gatctcctcc ttgtcgggaa tgccgtcgcg
     6121 gtccgtatcc agccccacgt ccatgtccgg cgggatcggc tgtttccctc cgcagaaggg
     6181 gcagacctcc gccgcatagg ggatgggttt cttgcaagcc tgaatcacac agaccacccg
     6241 gcgttccgga gtcagaaatc cggctttctg ggtttccgcg gtatccaact ggagcggact
     6301 gcgcaccagc cgtaccgcgt catcaaactc cgccatctca atagcggcca acggcgcggc
     6361 ggtgggtttg aggctttcga gctggcgcgt gtactgggac tcggcctgtt tgcgggccgc
     6421 accgacatgg gtgaggtaga aaagagagat cagcaggaaa atgagcacac cgatggcaat
     6481 cagcttgtca tagtgccgca acagggggaa gctggaaagt tttatggcca caggttacac
     6541 tccctcaaac gaatacacat cgatattcag cctcacgttg accggcgggt cgagatccgg
     6601 gtcggttacg atccgctcag catgcgggat cgtagcgagg tccacctcct tggtcgcggc
     6661 atcggcggca gacgcggccc cctctttttt cctggtgtcg tgccgtccga tggggtcatc
     6721 cgtcttgcga aattcggcct ccgcaaccac cacgaacagg tccatggccg ccaaacggtt
     6781 gagcgcccca atgaatgcgg ggggacgggc gagaaactcg aacgagaagc gttgtttgga
     6841 gaagaactcc ccggacgccg ctttccgggc tgcgggcgcc gcggcctcct gcgcctgagg
     6901 ctcgtcccga cgccgacgac gacgcgacga ggaggcctcc tgcgaccggt ccggaccacc
     6961 ggcctcggtc tgcgcgtcaa aggtttcgcg ctgcaccgcc ttgatctcca gaacaccagc
     7021 ctcgcacagc tcattgcaca accgctcgat gatcgtcaac tgaccggtca gacggtccac
     7081 atgctctggg gcgggcaggc tgtccgactc gccgaggtat tgatcgaagc cgaacaggaa
     7141 cccgggcgcg acgatgcgcc cgttggcggc gccgggccgt gccgacagcg cgcgcaccgt
     7201 ggcctgcagc gtctgcttga acccggtggg actcttcgac tcgatgtgca ggtcaccctg
     7261 gtgcagcagg ttggaagccg tggccagcca agactccagc gccttctgat cctcctttac
     7321 ccgtgcgatg gtatccggat cggggaagac cttgcctcgg tagattttct gcacggcggc
     7381 atagtcggca ttgcgcaccg aggccgcctc cttattggcc atccatgcgg aaaccaagaa
     7441 ccaccccgcg ccgaggcata cccccaccgt cagcacagcc agaatgatca aaatgagttg
     7501 ttgccgcttc atcgtgccgc ctcatccttc gggccggacg ccgccagcgt cgcctcgatt
     7561 gtgaattcga tcagatacgc cccgatgtcc ttgacaccca cgatacgcac cccctggggt
     7621 tcaccgctga aaaccgattg ggttttaagc cggtcacgca actcctcgac cgccgtggcc
     7681 gtgcggccgg cggatttggc cttctcttcg atctccttgt gcttgtccgc ccagccgcgg
     7741 ccgacgatgc gcagtccggt gacggtgccc gccgcatcgc gcaccggcgc gagttcggtc
     7801 agccacatac cctcgaaaag actcttgcgc acagcgtcca cacgcttcag ccaagcggtc
     7861 cgacaccaca tcagatcccg aatcgcctct gccttctccc gcacggcctc gcggcttttt
     7921 tcggcctgcg tcagcgaggc ctgctttgcc ttgcacacgc tcagcttggc cgcgacctgc
     7981 tctccttgga aggcatagag tttgcccatc tgcttgaggt agagcgtcca gatgccgaga
     8041 ctcagaaaca cgcccgccgc agccaaaccg aaaaacggga tgcgccgctt cagtgtcttg
     8101 cgctttgtga tttccggcgg catcaagtta atctcaaccg ggcacgccag cgaacggcgc
     8161 aacgccagcc ccacggtctc ggccagcaca aatgcgtcga cgccagcctt ttcacggtcg
     8221 atccggttgc tcaccgccac attcgtgaag gggttgaggt agagcacttc aacctgaagc
     8281 ttttcccgga agaacgtgtc gagatgcggc atcaccgacg accctccggt caggaagatc
     8341 cggacgggcg cattgccgcc ctgctggcta cggtagaaat tgatcgaccg gctgatctcc
     8401 gcgtgcaggc gggtgaccac attgcgcacg actttggaga cgcgttcgga aacctcgtcc
     8461 tcggaagccg aaacaccccc cagcgagaca aaggctcgtt cgcacttcag ggcctcagcc
     8521 tcggcaaagg agatctgaaa acttttagcc agttcttgcg tgatggcgtt acccgccacg
     8581 gggatgcagc ggttgtagat cttctcgtcc tccaagaaga tcaggttggt ggaacgcgca
     8641 ccgatgtcca gcagcatgga gcatccgtcc aactcggcat agttgtagcg cagacagttg
     8701 tacagggtca tgggcgcgac atcgacggtc tccggatcga gcccggcctc caccacgcgg
     8761 tcggtgatgt cacgcacgtt ttcgatcttg gccgccacga tcatcgcgca ctgctcaccg
     8821 gtggaggcgt cgccgatgaa ctggtggttc caaacgattt cctccatggg gaagggtacg
     8881 ttctgctcca cctcgtactg caccatctgc agcagcttat cctcgccgac agcggggagg
     8941 cgcacgaaac gggggaacac catctgtccg gagagcgaca acatcagcgg cgcgttccgg
     9001 ataccatgcg tccgcatgat ctcgcggatc gccggaacca gttgcgcacc ggtttcggac
     9061 tcgttctccg gctcgacgcc gaagacatgg gttccgtaat tgagcagttc cggcggcctc
     9121 ccgcttttga cggagaattc ggccagaacc aatttgctcg caccgacatt cagcgcgagg
     9181 atgcgatccg tcgagaacac tattgcacca cctcgtaatc gtccgcgttg atcagggcga
     9241 acggggtctt tgaacggtca agcaggccag tgcggcgggt tagcgcggca ttgtccagca
     9301 ccttgctgtc cttccaccac tctttcggga gggggatcgt ggattcggac ttgctgtcca
     9361 tcaccttgcc ctccttgttc gtgatctcca gcgccacggc cacgggctcg ccatgccgtt
     9421 cgacctgact cgggggaagc gcgacacaac tcatgtgctc gcctttggag atgtcaatat
     9481 accggaccgt aagggtgtag tagctgaacc cctctttgcc ttcttcgtcc ttgcccttgg
     9541 tcatgacatg ataggtgaag accaactcgt cggtccactt cgcgctgctg ttgtacttga
     9601 cttcgaacaa cgcccattcg cgcggccgac gggtgacttt cggcatcgtg ttctggacat
     9661 tcacgttgaa ttcgggggtg cgcaccatcg ccgtcttgtt gggaggaggc atgcgtgtga
     9721 tgttcacctc ctcgcgcttc tccgtccgca cagccgttgc ctgacccata cgcgtctgcc
     9781 cacggctcat ctgggccata gccgcttccg tacaaatcag catggccacc gccacgccca
     9841 tcacgctcat acccgtccgt ccgacaaccg tcttgttcat gatcgttctc ccaatcgtga
     9901 atccaacctc tccacgcaag ccgctgttac cacatgtagt gcgtacaatt taatgcggag
     9961 tgagacggaa tgcaaccctg aaacagaaaa agttcttcat cgggacatat cccttgttgc
    10021 cgcaacgggt gcggacaagc gaaaacggga atgaaaacag gggggggaac gcgcctgttt
    10081 gcccgtcgtc cccgtcctct cgtgtacgtg cacgggcacc gcttcgctgt gtacgggcag
    10141 ggtccgaagg cacaacggtc aattcgggaa tgcgcgatga ccagattctc tccgccccgc
    10201 cgcttgacat cccgggtgcg atggattaca gtgcgcccct tcctgcggtg cgtctcagga
    10261 gacactcccc gctgcgggtc atctggcgct tgcgtccgga tttcgcttta cacccggcgg
    10321 ggggggactc tatagtgtgc gccggaaagt aacgacaaaa aagaaagaaa tcgcggaaca
    10381 aggaccgtaa agcagactat gagccagatt gacaagaccg ctatccgtaa gcaattccaa
    10441 cgccatgacc gggacaccgg atcgagcgaa gtccagatcg ccgttctcac caaagagatc
    10501 gagatgctca ccgagcacct caaggcgaac aagaaggatc acagctcgcg ttatgggctg
    10561 atccgtaagg ttaacacccg ccgtagcttg cttgactatc tcaagcgcga agacgaggcg
    10621 gtttaccaga agatcatcaa agagctcggc ctccggcgtt aatcgtgcgc gaaggcggcc
    10681 gtgcgcctgt cgcgcgcggc cggaagcgcc cggaccgaca tcccgggcgc gctttcagac
    10741 ggacctctgc acttgttcga ataggccatc tttcctggcc aaacgctttc cggtccttgt
    10801 ccgctgaaag gtgacacacg atgaaggata caacctctgt ctccgtccaa gtgggcgggg
    10861 ccacgatgac cattgagacc ggactgctcg cgcgtcaggc tgccggagcc accgtctgcc
    10921 gtctgggtga ttccctgatg ttctccgctg tgacctgcac ggacaagccg cgcgagggga
    10981 tcgacttctt cccgctgcaa gtcgaatacc gcgagaagta ttacgcggcc ggccggttcc
    11041 ccggcggatt tttcaaacgc gagtcgcggc cgagcgagaa ggaaatcctc acggcacgca
    11101 tcaccgaccg ccccatccga ccgctcttcc cggagggcta tcggaatgat gtccagatca
    11161 acaacatggt gctctcggca gacaccgaga acgacaccga catcctttcc gtcaacgcct
    11221 cctcggctgc cctccatatc tctgatatcc ccttcatggg gccgatcggc tgtgtgcgtg
    11281 tgggccgtat ccatggcgaa ttcgtgatca accccacgca ctcccagcgc aaggagagtg
    11341 accttgactt ggtctacgcg ggcacgcgcg agcgttttct catgatggaa ggtggcgccg
    11401 cggagattac cgaagaggat ttcctcgccg cgatgaaggt cggccatgca gaggtcgtca
    11461 aaatcatcga cgcccagcac gagttgcgcc ggttgctcgg caagccggaa aagaagatcg
    11521 aagaggtcgc gccgaacgcg gagaaaatgg cgttcctcta tgccgagggc gagaccgctc
    11581 tgcgtcaggc cctgctcatc gtcgacaagc tggaacgcca gaatgcggtc aatgcgatca
    11641 aggaagactt gcaggccaag gcccttgcaa agtggccgga agaggtcacc gccgcatcct
    11701 tcgtcacgct cttcgacacg ctggagatcg acctcgtccg caggaatatc cttgaggacg
    11761 gcaagcgcat cgacggacgc gccgccgatg aaatccgcaa gctctacgcg caggttggcg
    11821 tcatgccccg ggctcacggc tcggcgattt tcgaacgcgg cgagacctcc gccttggggt
    11881 cggtcacgct cggcaccaaa aaagacgctc aggaactcga tgccatcacc ggcggcgaaa
    11941 ccagcaagtc tttcttcctt cactacaact tcccgcccta cagcgtcggc gaggtcggca
    12001 ggctcggcgc cacgggacgc cgcgagatcg gccacggcgc cttggccgag cgttcgctcg
    12061 cccaagtcat gccgcaggat tacccgtaca gtatccgcgt ggtctccgac atcatgggct
    12121 cgaacggttc ctcctccatg gcctccatct gcgttggatc gctggccttg atggatgcgg
    12181 gcattccgat caaggctccc gtcgcgggtg tctcggtcgg cttgttcaca aataaagacg
    12241 aaagccagaa gattctggtg accgacatct tgggagcgga agaccactgc ggcgacatgg
    12301 acttcaaggt ggccggaacc cgcaagggga tcacgggctt ccaagtcgac ctgaaactgc
    12361 gcggcctgac ctgggatgtg gtagaagccg ctctcgcgcg cgccaaacag ggacggttgc
    12421 agatcctcga cttcatggag tccgtcatgc cggctccgcg cgccgaactc tcgcagttcg
    12481 cgccgcgcat cacggtcatg acgatcccgg tggataaaat cggggcgctc atcggcccgg
    12541 gcggctcgaa catccgccgc gtgtgcgaga tctccggcac ccagatcgac attcaggacg
    12601 acgggtcggt cagcatcttc gccaacaacg ccgagtcgct ggccatcgcg cagaacgagg
    12661 tcttggcgct caccgccgaa gcggaagagg gcaagatcta cgaaggcacc gtcaccggca
    12721 tcaaggagtt cggctgcttc gtcgagatca tgcccggcaa ggacggcctc tgccacatca
    12781 gcgagctcgc ggaccgccgt atcggctccg tcgaggatgt ctgcaaggtc ggcgacaaga
    12841 tgacggtcaa gtgcatcggc gtggacgacc gcggccgcat caaactcagc cgccgcgaag
    12901 ccatgcgcga cctcgaccag cagaaacaga acggctgaac gccgcgctca tcgtaatctg
    12961 aaagcccgcg ataataccca tcgcgggctt tttgttggtt tctgacccgc gacactcgac
    13021 ccgcgacgtg gcacgggcat cttgcccgtg ttgcatgggc gaaacgccca tgccacgaat
    13081 acactccccg cccgcgttcc cgacaccgca agtcgcccgt cgcgggtcaa tccctaactc
    13141 tcggctatct ccgcgctacg cacggagttc aaccgtagat cggaccgaag ttcttgcagg
    13201 cgcggtagtc cgcgccgatc ggatcgtcga tgccgaacag accccaggcg tgcggccgga
    13261 agatctgctc ttcgggcacg ttggacatcg caaccggaat gcgcagcatg gccgcaagcg
    13321 tggtcagatc cgcgccgatg tggccgtagc tgatcgcgcc gtggttggcg ctccagttgt
    13381 tcatgacgct gtagacgtcg cggaaagcgc ccttgcccgt caggcgcggc acgaaccacg
//
LOCUS       DAOSRB010000067        12929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4850_length_12929_cov_6.508467, whole genome shotgun sequence.
ACCESSION   DAOSRB010000067 DAOSRB010000000
VERSION     DAOSRB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4850_length_12929_cov_6.508467"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLZ74_05185"
     CDS             complement(<1..217)
                     /locus_tag="PLZ74_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOR97750.1"
                     /translation="MRRLWVTVLCVAAMTYAEPVKLIFDTDMGNDTDDLMALCMIHTL
                     QSRGAVDLLAVTITKDHPQAAAFVDAVN"
     gene            complement(264..1145)
                     /locus_tag="PLZ74_05190"
     CDS             complement(264..1145)
                     /locus_tag="PLZ74_05190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR97751.1"
                     /translation="MKTVKVNGQRISEEAIQFELERLVRFHAEHGMAEDQVRAQLPAL
                     VDKAIDQAIGAKLLLDEASRLDIPVREEDVEAQIARIIEQVGGEDAFRKALTGQNTTE
                     QVFRDQIRRGCRVEKLVEQAVAGVAEPTEEDIRRHFDEHKEEYAPGERVLAQHILISP
                     DGDTQTSKNEARAKIEAIRERIQAGKSFSDEAAAHSMCPSGKDGGSLGWFSRGMMVPE
                     FDSAAFSMNVGEVSDVIETQFGYHIIFKTDHEAAGEADFDQVREKIRDFLRHARRGEA
                     MAAYVNELKSKAKIEVE"
     gene            1434..1685
                     /locus_tag="PLZ74_05195"
     CDS             1434..1685
                     /locus_tag="PLZ74_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR97752.1"
                     /translation="MPVYVYEAVNPDAGCPHCVGSFDVTQSIHDARLTACPACGAAIR
                     RVITAPAIGRSKSKLDDRAKSAGFTKLKRLGKGEYEKIY"
     gene            1696..2607
                     /gene="epmA"
                     /locus_tag="PLZ74_05200"
     CDS             1696..2607
                     /gene="epmA"
                     /locus_tag="PLZ74_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOR97753.1"
                     /translation="MNPERLALRSRILHAVRAFFHAHDFVEAETPVRIPAPALETHID
                     VPPAGRAWLRASPELHLKRLLAAGSPRLYQIGPCFREGECGRRHNPEFTLLEWYRAHA
                     DYIAILRDTEALLTQVFQSVFGRTTLVYQGVKIDLEPPWTCLSVREAYQRFAGWDPVR
                     DWDPERFDLDMVHAVEPALPRDRPTVLTDYPAEAASLARLKPDDPQVAERWELYIGGL
                     ELANAYSELCDAETQRARFLAAAEARRQLGKVIYPLDEPFLDALRSGMPPCGGIALGI
                     DRLIMLACDASDITEVRPFCQRPGELW"
     gene            2751..5885
                     /locus_tag="PLZ74_05205"
     CDS             2751..5885
                     /locus_tag="PLZ74_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97754.1"
                     /translation="MLILLRMILLFLGPVFLFVALLPTLLSTDAGRRFVLDRLNAAGD
                     IRLTVEKWSWGWFRAPVLEQVSLIDPERGMTVTAERLTSDRGLWRLLPLGTWNAGNLT
                     LEKPEVTLDLMPRPRADAAKPREPSRSRDGGFFLPILDIAAHAVIREGSLRVNGQSET
                     PFEAQQIEGQITLTSFRKPIEINGQMRIGGGTLGIGGRVQSLRALTAARGAPPAEAEA
                     LTVKLLGVDLTAVRPLLREWAGQEWVSGGKAEGAFTVTMTNLDHLTLKGGVLVTRLTV
                     AAAGHRPSPPGDLALMTDLTYERPSLTITRFDLSAPWVRAEASGALQTAGQGKTVPIG
                     TLRAKIAVDLAAVARDFGPALNLAKGLRVRQGVLQGTVAVQGDETALGLDAALAIADL
                     NLTVDGQPLILKPEPSLHLKASFPYGHGPTVETLRLKAPFAELYGSGRFDSAVVKGQV
                     NLTRFSRDFKRILHSVPPMVGSIYLDLATRREKGHAAVTGFLKVSDTSVEVRPGQRLT
                     VPLGTLKLNSRVPMKGDQPERELQDTTFDVILDKTKLAGGWKRWRPAQSGQPTVLRGF
                     TLAADTDLHSLRKTLGGLLPPPTLRRLASWQGALVANATAEAASGVVKARMNAALRQG
                     AARTAAGIWRVPDLRMEGTLSQSVQQPGLRCETTATGSAAFERDGETVYAEPAARLVT
                     DFTLAADGQSVRIDTFDIRSSLIEARARGSLSDLAVRRLLQAQGDMTVDFAAVTRLLD
                     ARGIDEFTLTGKAARPFRFSAPTAGGLTTLLAEGEFSGAAFLASAKGLGLDAGPSDVN
                     LRLAKGVLDTRYAPTLNGGRMVFVPQLRYTPDGVSLTVPEKSRLLENVKITQQMVDNL
                     LVNLNPMFHGSKIRDGTVTLDVRHFTYRPGTDLKTGLSADLSLRFDRLSLSLGPALRE
                     VLTFLKVKEQTYTVEHLPVHLVIRDGKTHVDPIRMVVGNQPIVFSGWVAFDGSIRYLL
                     QVPITERLTGGAGKMLKGTTISIPITGTVTQPRVDLNVLHNALGGLIKQTVGEENLQK
                     VQSFLEQLREEFRK"
     gene            complement(5895..7262)
                     /locus_tag="PLZ74_05210"
     CDS             complement(5895..7262)
                     /locus_tag="PLZ74_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97755.1"
                     /translation="MRTKFGIMRVTVAVLWACGVRAAEYTGPAEWRMVNTNGVRAWQA
                     AQQAAASGGTVAVWPGVAADPVRREVRLLAEAVGHTEGVTTEFILIGPMSDRAYEAVA
                     VTVAAPSDIVRAVEHLGLKRGGGVGSRPFRFWPVGERVTATVRRLDGADAAVLPLQTL
                     IRDEGEGEPLVGEGGLVFTGGRWVGPSCLTDTNMPCSVISLYNAGETVFDVPFRVGQS
                     EVYGRLKLARSYPCGALIEVVLRPLAADGRPQVLSLRVTAEPGEGGVSLVTRGADGTV
                     LHRSALAEGLVWLRKQADTGRELFVTLEMDETLTLQQATEVARIFDMLDGKGLKLDGK
                     GEDGVYAKAFLPLERWREREGRTPQPFELHVKRNAEGGWTRSLVFIEEDWQVEGLDPR
                     LTPRTYPFEEWEALPGLTARAGGAENKVRVLFVFAPREASLRTFMPGVRALSGRLPIV
                     YVFGE"
     gene            complement(7294..8478)
                     /locus_tag="PLZ74_05215"
     CDS             complement(7294..8478)
                     /locus_tag="PLZ74_05215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOR97756.1"
                     /translation="MQVIAKAVQEQMGSASWIRKMFEKGAELKKAFGDEQVCDFSLGN
                     PDVPPPAKARDALCALADQAVRPYGLGYCPNAGLPAFREALADFLARQQDVPLRAEQV
                     VVTCGAAGALTSFFRAVLEPGDEVICPAPYFVEYGAYGGHFGGVLRPVPALPPDFQPD
                     VAAIEAAVTERTRVLLINSPNNPTGCVYTRETLERLGALVDRVNAARERPLFLVSDEP
                     YRFFAYDGVTVSPVLPFSPFSLVVGSFSKSLSLAGERIGYLAANPAMPQVKTLMDAVT
                     MTSRTLGFVNAPVLGQRLAMALLEEGVNLDVYDRRRQAMAAVLTQAGIRFAMPRGAFY
                     FFPEAPGGDDLAFVQRLLERNVLVVPGRGFGFPGYFRLSFCVEEAVIARSAEGFQAAV
                     AG"
     gene            complement(8469..8834)
                     /locus_tag="PLZ74_05220"
     CDS             complement(8469..8834)
                     /locus_tag="PLZ74_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOR97757.1"
                     /translation="MARVKQKRDGKSFRAVARVLAEINSTEEMAAFLDELLTPAEVYD
                     ISLRWKLLGLLAQGMSQRRIAEELRISLCKITRGSRILKQKGAVSARLLSDGKTDGTA
                     YGKTGGLKRNIRKGVGGCK"
     gene            complement(8850..9695)
                     /locus_tag="PLZ74_05225"
     CDS             complement(8850..9695)
                     /locus_tag="PLZ74_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97758.1"
                     /translation="MRRVLTGVAAVWLGCAVVSGQSWDATDQAEIDLGVTTGVPQASG
                     FVFIEGRYLAPPYTVTRKGNGLFINRTLVERPVPWPPAVTPQVPGTEKRVDGDGAAQP
                     QAEPQNEAPQIGERKKVLSVDDLFDDEDSDEETVPLAEDEVKRPVAEATAAAGNGLSA
                     EEIARRKAETLAVLERLRKGYEVALSQGEVFFFSQRHNRVNGTYGTARLLMGVLPAAL
                     RYAKSPQDLMSRLYQGGITFIDLTMCAALYGHRNTFPLLEARLARIEEAEREEERKRN
                     AVRRR"
     gene            complement(9711..9888)
                     /gene="ssrS"
                     /locus_tag="PLZ74_05230"
     ncRNA           complement(9711..9888)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLZ74_05230"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(9949..12363)
                     /gene="pheT"
                     /locus_tag="PLZ74_05235"
     CDS             complement(9949..12363)
                     /gene="pheT"
                     /locus_tag="PLZ74_05235"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOR97759.1"
                     /translation="MKVPVSWLNEFVDVSDLSVRELADKLTFSGVEVEGVEEVGVILD
                     DAFVVGEVLTCAAHANSDHLHVCTVADGEQEWQVVCGAPNVRAGLKVAFARIGAVIPE
                     GGFTIKKAKLRGVESFGMLCSARELKLSDDHAGILELDAALKPGTPLRDVLPQPETVL
                     DLEITWNRPDCLSIIGIAREFAALLRRPLRLPPVDFAETEEPVESFAKVRVEDPVKCP
                     RYTARVMTGVRDGASPAWMQHRLELCGVRAISLTVDVTNYVMLECGQPLHAFDYRTLA
                     DHTVVVRCATPGETITTLDGVKRTLDPSMLVIADAKAPSAVAGVMGGAGSEIVTDSEH
                     VLLESALFDPAATKFTATRLGIGTESSRRFERGVDPDLADWASRRAVALLAEHGQARV
                     ARGVIDVDNRGWKAGEVTLRFARAREVIGVAMADDEMVGLLTSLGLEEIARDAQGATF
                     RIPSWRLDLTLEADLIEEVARLHGLDAIPDRLPDATAVSTLDEGAFRARSRCRQILLG
                     MGFSEAMHYSFLSAQELDAFDTRHAASRVVLPNPVSADYGVLRDTLLPQLAGSLGRNA
                     SRQVECAALFEMGRVFFKEANGVPGEEERVALGLMGPFGRSSVERRRPVGNEEALLWL
                     KGAVENLAAALHVGDLTFVRGEHPAMETGWAVGIELNGEQVGCMGLLSAALRHAWRMT
                     APMPLAELRLAPLLAGAGAAAAIQPVPQYPAVRRDIAFVADAAVTHREVVETIRRAGP
                     KELTDVGLFDIFQSKEIGKGKRSLAYALEFRSPDRTLTDQEVNAAFAEIARALKQNLH
                     VEVREG"
     gene            complement(12565..12774)
                     /locus_tag="PLZ74_05240"
     CDS             complement(12565..12774)
                     /locus_tag="PLZ74_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97760.1"
                     /translation="MIRACRIVAGPGRWRASTPQEKGSYEGDAVTFVADTGERVSFRV
                     TAGKSAPRITLKTMWIEKQKDRPAD"
BASE COUNT         2434 a         4613 c         3559 g         2323 t
ORIGIN      
        1 tgttgaccgc atccacgaac gcggcggctt gcggatgatc tttcgtaatg gtcacggcca
       61 gcagatcgac cgcgccacgc gactgcaacg tgtggatcat gcacagtgcc atcaggtcgt
      121 cggtgtcatt ccccatgtcg gtgtcgaaaa tcaacttcac cggttcagcg tacgtcattg
      181 cggccacgca gaggaccgtc acccacaggc gtctcatgtt cttcatctcg ttctccttat
      241 acccttttgg ccatcacact cgctcactcc acctcgatct tggccttgga cttcagctcg
      301 ttgacatagg cggccatggc ctcgccgcgt cgggcatgcc gcagaaaatc gcggatcttt
      361 tcacgcactt ggtcgaaatc cgcctcgccc gcggcctcat gatcggtttt gaagatgatg
      421 tgatacccga actgcgtctc gatgacatcg ctgacttcgc cgacattcat tgaaaaggcg
      481 gctgaatcga actccggcac catcatgccg cggctgaacc agccgaggct accgccgtcc
      541 ttgccgctcg ggcacatcga gtgcgctgcc gcctcgtccg aaaaagattt gccggcctga
      601 atgcgctcac gaatcgcctc aatcttggcg cgggcctcgt tcttcgaggt ctgcgtgtca
      661 ccgtccggcg agatcagaat gtgctgagcc agaacccgct cgcctggagc atattcctcc
      721 ttgtgctcgt cgaaatgccg ccggatatcc tcttcggtcg gttccgcgac tcccgccacg
      781 gcctgctcca ccaacttctc cacccggcac ccgcgacgga tctggtcgcg gaacacctgt
      841 tccgttgtat tttgtccggt cagggccttg cggaatgcat cctcccctcc gacctgctcg
      901 atgatccggg cgatctgcgc ctccacatcc tcctcgcgaa ccggtatgtc gagacggctg
      961 gcctcatcaa gcagcagctt cgcaccgatg gcctgatcga tcgccttgtc aaccagagcc
     1021 ggcagttgcg cacgcacctg atcctccgcc atcccgtgtt cggcatggaa acggacgagc
     1081 cgctccaact caaactgaat cgcctcttcc gaaatccgct gtccattgac cttgactgtt
     1141 ttcatcccgc tctttctgtc ttatcggcac atttggcttt ggggcccttt cgtccttctt
     1201 tccgctccgc atcttagcaa gcgcccccct tcgattcaag cgcgggcacc ctctttcgag
     1261 ccgcgtaccc gtctcgatcg ggtttcccgt ggtcagggct ttcttcgcat catcgctagc
     1321 gccccccaaa aaaccttcgt gtgagaaatc tggaatccga aacccgaaac ctcaactttt
     1381 cctgccggat tatgctatag tgtgacgcat cggaacccat ccggagaccc aacatgcctg
     1441 tctatgtcta cgaggccgta aatcctgacg cgggttgtcc ccattgcgtc ggcagcttcg
     1501 acgtcacgca atcgatccac gacgcgcgcc tgacggcctg tcccgcctgt ggcgcggcca
     1561 tccgccgggt gatcacagcc cctgccatcg gccgttccaa atccaaactc gatgaccgcg
     1621 ctaagtccgc gggcttcacc aaactgaagc gcctcggcaa aggcgaatac gagaagatct
     1681 attgaacggt tgatcatgaa tcccgaacgc ctcgccctgc gcagccgcat tctgcatgct
     1741 gtgcgcgctt ttttccacgc ccatgatttt gtggaggcgg agacgccggt acgcattccg
     1801 gcgcccgcgc tcgaaacgca catcgatgtg cctcctgcag gacgcgcatg gctgcgggcc
     1861 tcccccgaac tgcatctgaa gcgtctgctc gcagccggtt caccacggct ctatcagatc
     1921 ggtccctgct tccgcgaagg cgaatgcgga cgacgccata acccggagtt caccctgttg
     1981 gagtggtacc gcgcacatgc ggattacatt gcgatcctcc gggacacgga agcgttgctg
     2041 acgcaggtgt tccaatccgt gttcggccgg acaactctgg tttaccaagg ggttaaaatc
     2101 gatctcgaac ccccgtggac atgcctgagt gtccgcgagg cctaccaacg tttcgctggt
     2161 tgggatccgg tacgtgactg ggatccggaa cgattcgacc tcgatatggt gcatgccgtc
     2221 gaacccgccc tgccgcgcga ccggcccacc gtgctgaccg actatcctgc tgaggccgcc
     2281 tcgctcgccc gcctcaagcc ggatgacccg caggtggccg aacgctggga actctatatc
     2341 ggcggtctcg aactcgccaa cgcgtacagc gaactctgcg atgccgaaac ccagcgcgca
     2401 cggtttctcg ccgcagccga agcccgccgt cagctcggca aagtgatcta tccgcttgac
     2461 gagccgtttc tcgacgccct gcgttcgggc atgcccccct gcggagggat cgcgctcggc
     2521 atcgaccggc tgatcatgct ggcctgcgac gcatccgaca tcaccgaagt ccgccccttc
     2581 tgccaacgtc ccggcgaact ctggtgacgg catcgacgag accgcgtgaa catgatatgc
     2641 ttctcccgat cttatgagcg ccccgcgcga accggaaagg caacgaaatg gatttcacgg
     2701 agatccgaaa cgacgacacg gaagagaccc tagcgcatcg gccagggtgt atgctaattc
     2761 tcctccgtat gatcctgctc tttctcggcc ccgttttttt gtttgtggcg ctgctgccca
     2821 cgctgctctc aaccgatgcg ggacgacgtt tcgtgcttga tcgcctgaac gcggcaggcg
     2881 acatccgcct cacggttgaa aagtggtcgt gggggtggtt ccgtgccccg gttcttgaac
     2941 aggtcagcct gattgatccc gagcgcggca tgaccgtcac cgcagaacgc ctgacttccg
     3001 accgcggtct ctggcgcttg ctgccgctgg gcacatggaa cgcgggaaat ctgacgctag
     3061 agaaaccaga ggtcactctg gacctcatgc cacgcccccg tgcggacgcg gcgaagccgc
     3121 gggagccctc ccgctcccgc gacggcggtt tcttcctgcc gattctggat atcgcggcgc
     3181 atgccgtgat ccgggaggga tcgctgcggg tgaacggcca gagcgaaaca ccgttcgagg
     3241 cgcaacagat cgaaggccag atcaccctga cctcgttccg caaacccatt gagatcaacg
     3301 ggcagatgcg gatcggcggc ggcacgctcg gcatcggggg acgcgtgcaa agtctgcggg
     3361 cgttgaccgc tgcccgcggc gcgccacctg ccgaagcgga agcgctgacc gtcaaactgc
     3421 tcggcgtgga tctcaccgcc gtgcgcccac tgctgcgtga gtgggccggt caggaatggg
     3481 tcagcggcgg aaaggcggaa ggcgccttca cggtaacgat gacgaacctc gaccacctga
     3541 ccctgaaagg cggcgtgttg gtcacgcggc tcacagtcgc cgctgcggga caccgcccct
     3601 cgcccccggg cgatctggcg ctgatgacgg atctcacgta tgagcgtccc tccctcacca
     3661 tcacccgttt cgacctctcc gcgccgtggg tccgggccga ggcatcgggc gcgttgcaga
     3721 ccgctggaca gggcaaaacc gtccctatcg gaaccctccg cgccaaaatc gcggtcgatc
     3781 tcgcggctgt ggcgcgggac tttggtccgg cgctcaacct cgccaaggga ttgcgtgtgc
     3841 gtcagggcgt gctccagggt accgtggcgg ttcagggcga cgagaccgcg ctgggcctgg
     3901 atgcggcgct cgctatcgcg gacttgaacc tgactgtgga cgggcaaccg ctgatcctga
     3961 agcccgaacc ttcgctgcac ctgaaggcat ccttccccta cgggcacggg cctacggtgg
     4021 agaccctgcg gctcaaagcc ccgttcgcgg aactttacgg cagcggccgc ttcgactccg
     4081 ccgtggtgaa aggccaagtg aacctaaccc ggttttcacg cgatttcaaa cgtatcctgc
     4141 actcggtccc gcccatggtc ggttccattt atctggatct ggccacccgg cgcgagaagg
     4201 gtcacgcggc ggtcaccgga ttcctgaaag tttccgatac atcagtggaa gtccgtcccg
     4261 gccagcgcct gaccgtgccg ctcggcacac tcaagctcaa cagccgcgtc cccatgaaag
     4321 gcgaccagcc cgaacgggag ttacaagaca cgacgtttga cgtgattctg gacaagacga
     4381 aactcgcggg cggatggaaa cgctggcgtc ccgcgcagag cggtcagccg accgtgctgc
     4441 gtggcttcac gcttgccgca gacacggacc tgcactccct gcgtaaaaca ctggggggtc
     4501 tccttccgcc tccgaccctg cgccgtctgg cgtcctggca gggcgcactg gtggcgaacg
     4561 ccaccgctga agccgcctcc ggtgtggtca aagcgcggat gaacgcggca ttgcgccaag
     4621 gtgccgcccg gaccgccgcc ggcatctggc gcgtgccgga tctccgcatg gaaggcaccc
     4681 tgtcgcaaag cgtgcagcag cccggcctgc gttgcgaaac cacggccacc ggcagcgcgg
     4741 cattcgaacg cgacggcgag actgtctatg ccgaaccggc cgcgcggctc gtgacagatt
     4801 tcacactggc ggccgacggc caaagcgtgc ggatcgacac cttcgacatc cgttcgagcc
     4861 tgatcgaagc ccgcgcgcgc ggatcgctct ctgatctcgc ggtgcggcgt ctgcttcagg
     4921 cccaaggcga catgaccgtc gatttcgccg ccgtgacgcg ccttttggac gcccgcggaa
     4981 tcgacgagtt caccttgacg ggcaaagcag cccgtccgtt ccgtttttcc gctccgaccg
     5041 caggagggtt gacaaccctc ctcgccgaag gagagttttc cggcgccgcg ttcctcgctt
     5101 ccgcaaaagg cctggggttg gatgccggac cgtcggacgt gaatctacgc ctcgccaaag
     5161 gggtgctcga cacgcgatac gcgcccacgc tgaacggcgg tcggatggtt ttcgtgccgc
     5221 aactgcggta caccccggac ggcgtatcgc tgaccgttcc ggaaaagagc cgtctgctgg
     5281 agaacgtgaa aatcacccaa cagatggtgg acaacctgct tgtgaatctg aacccgatgt
     5341 tccacgggag caagatccgg gacggcacgg tgaccttgga tgtacgccat ttcacgtacc
     5401 gacccggcac ggacctgaag accggactct ccgccgatct ctccctgcgt ttcgaccgcc
     5461 tgagcctgag cctcggccct gcgctacgtg aagtgctgac gttccttaaa gtcaaggagc
     5521 agacctacac cgtcgagcat ctgccggtgc atctggtcat ccgcgatggc aagacgcatg
     5581 tggatcccat ccgcatggtg gtgggcaacc agcctatcgt gttcagcggc tgggtcgcct
     5641 tcgacggctc gatccgctat ctgctgcaag tgcccatcac cgagcgtctg accggaggcg
     5701 cgggtaaaat gctgaagggc acaaccatca gcatcccgat caccgggacg gtcacccaac
     5761 cgcgggtcga cctgaatgtg ctgcacaatg cgttgggcgg cctgatcaaa cagactgtcg
     5821 gcgaggagaa cctgcagaag gtccagtcgt ttctggaaca gcttcgggag gaattccgca
     5881 aataggacgg cggttcattc gccgaacacg tacacgatcg gcagccgccc cgacagcgcg
     5941 cgtacgccgg gcatgaacgt ccgaagcgac gcctcccggg gtgcgaaaac aaaaagcacc
     6001 cgcaccttat tctcggcccc gcccgcacgg gccgtcagac ccggcagcgc ttcccactcc
     6061 tcgaacggat acgtgcgcgg ggtcagccgc ggatcgagac cctccacctg ccagtcctct
     6121 tcgatgaaaa ccagacttct ggtccagccg ccctccgcgt ttcgtttcac atgcaattca
     6181 aagggctgag gcgtgcgccc ctcacgctcg cgccacctct ccagcggcag aaaggccttg
     6241 gcgtaaacac cgtcttcccc tttgccgtcg agtttcaacc ccttgccatc cagcatatcg
     6301 aaaatccgtg cgacctccgt agcctgttgc aacgtgagcg tctcatccat ctccagcgta
     6361 acgaaaagtt cacggcccgt atccgcctgt ttccgtagcc agaccagccc ctcggccaaa
     6421 gcggaacgat gcagaaccgt cccgtccgcc ccgcgcgtaa ccaatgacac cccgccctca
     6481 ccgggctccg cggtcaccct cagcgacaga acctgcggcc gaccgtccgc cgccaacgga
     6541 cgcagcacga cctcgatcaa cgcaccgcac ggataagagc gcgccagttt cagacggccg
     6601 tacacctcac tctgcccgac ccggaacggc acgtcgaaaa ccgtctcccc cgcattgtag
     6661 agcgatatga ccgaacaagg catgttggtg tccgtgaggc aggacggacc gacccagcgc
     6721 cctcccgtga aaacgaggcc gccctccccg accagcggtt cgccctcccc ctcgtcgcgg
     6781 atcagggttt gcagcggcag cactgccgca tccgcgccat cgagccgccg caccgtcgcg
     6841 gtcacgcgtt cccccacggg ccagaagcgg aagggacgac tgcccacccc tccgccacgt
     6901 ttcagaccga gatgctcgac agcccgcacg atatcgctgg gcgcggccac ggtcacggcc
     6961 accgcctcat aggcacggtc actcattggc ccgatcagga tgaattccgt tgtgacgccc
     7021 tccgtgtggc ccacggcttc agccagcaac cgcacctcgc gccggacagg atccgccgcg
     7081 acacccggcc agaccgcgac ggtgccgccg gaagcggccg cctgctgtgc ggcctgccac
     7141 gcccgcaccc cgttggtgtt gaccatccgc cactccgcag gcccggtata ttctgcggcc
     7201 cgcacaccgc atgcccagag caccgccacc gtcacccgca tgattccgaa ctttgtccgc
     7261 atgctccctc ctttcggcaa cgtcctcgta gcctcagccc gcgaccgcgg cttggaaccc
     7321 ttcggccgaa cgcgcaatca ccgcctcctc cacacagaaa ctcaacctga aataacccgg
     7381 aaagccaaag ccgcgacccg gtacgaccag cacattacgc tccagcaacc gctgcacaaa
     7441 cgccagatcg tccccgccgg gcgcttccgg aaagaaatag aacgccccgc gcggcatggc
     7501 gaaacggatc cccgcctgcg tgagcaccgc ggccatcgcc tgacgccgcc ggtcatacac
     7561 atcgaggttc acgccctcct ccagcaacgc catcgccagc cgttgaccca ataccggcgc
     7621 attcacaaac cccagcgtac ggctggtcat ggtcaccgcg tccatcagcg tcttgacctg
     7681 cggcatggcg gggttggccg cgagataccc gatacgctcg ccagccaacg agaggctctt
     7741 ggaaaaggat ccgaccacaa gggaaaaggg cgagaacggc agcaccggag acacggtcac
     7801 cccgtcgtac gcgaaaaacc ggtacggctc gtcactcacc aaaaagagcg gccgctcgcg
     7861 cgcggcgttg acccgatcca ccaaagcgcc gagacgttcg agcgtctccc gcgtatacac
     7921 gcaacccgtg gggttgttcg gcgagttgat cagcaacaca cgcgtcctct ccgtcaccgc
     7981 agcctcgatc gccgcgacat ccggctggaa atccggcggc agggcgggca ccggcctgag
     8041 cacgccgccg aaatggccac cgtacgcgcc gtactccacg aaatagggtg cggggcatat
     8101 cacttcgtcg cccggctcca acaccgcacg gaaaaaggag gtcagtgcgc cggccgcacc
     8161 gcaggtgacc accacctgct ccgcccgcaa cggcacatct tgctgccgcg cgaggaagtc
     8221 tgccaaagcc tcacgaaacg cgggcagccc cgcgttcggg caatatccca gcccgtacgg
     8281 ccgcaccgcc tgatccgcca gcgcacacag cgcgtcacgc gccttggccg gcggcggcac
     8341 gtccgggttg ccgagactga aatcgcacac ctgttcgtcc ccgaaggcct ttttgagttc
     8401 cgcgcctttt tcgaacatct tccgaatcca agacgcgcta cccatctgct cctgcaccgc
     8461 ctttgcgatc acttgcatcc gcccacccct ttccggatat tccgtttcag cccgcccgtc
     8521 ttgccgtatg ccgttccatc ggttttcccg tcggacagca gacgggcgct cacggcccct
     8581 ttttgtttga ggattctcga tccacgcgtg attttacaca agctgattct cagttcctcg
     8641 gcaatccgac gctgcgacat gccctgcgca agcaacccga gcaacttcca tcgcagagag
     8701 atgtcgtaca cctctgcggg agtcaacagc tcatcaagaa aagcggccat ctcctcggta
     8761 gagttgatct ccgccaatac gcgcgccacc gcccgaaagg attttccgtc ccgcttctgt
     8821 ttcactctgg ccattttcca ctcctcccgt taccggcgcc gcaccgcgtt acgcttccgc
     8881 tcctcctcac gttctgcctc ctcaatccgg gccagccgcg cctccagcag cggaaacgtg
     8941 ttacggtggc cgtataacgc ggcgcacatg gtgagatcaa taaacgtgat gccaccctga
     9001 tagagccgac tcattaaatc ctgcggcgac ttcgcatacc gcagggccgc tggcagcacg
     9061 cccatcaaca gacgggccgt cccgtacgtg ccgttcaccc ggttgtgccg ctgactgaaa
     9121 aaaaacacct cgccctgcga caatgcgacc tcatacccct tgcgcagtcg ttccagcacc
     9181 gccagagtct cggccttgcg ccgcgcgatc tcttcagcag acaagccatt cccggccgcg
     9241 gcggttgcct cggcaaccgg gcgcttcacc tcatcttccg caagcggcac cgtctcttca
     9301 tccgaatcct catcgtcgaa caggtcgtcg acgctcaaga ctttttttcg ctccccgatc
     9361 tgaggtgcct cgttctgcgg ttcggcttgt ggctgtgccg ccccatcgcc gtccacacgc
     9421 ttttccgtcc ccggcacctg cggcgtgacc gcaggcggcc aaggcaccgg ccgctccacc
     9481 agcgtgcggt taatgaaaag accgttccct ttccgcgtca ccgtataagg cggagccaga
     9541 taacgcccct cgatgaacac gaaaccactc gcttgcggca cccctgttgt gacccccaga
     9601 tcgatctccg cctgatccgt cgcatcccac gattgaccgg acaccaccgc gcatccgagc
     9661 cacaccgccg cgacccctgt taaaaccctt ctcacacgtc ccccatccga aaaaaccccg
     9721 ctttgccgta tgacagcacc tctggaacct cacgaatgag gtgggcgctt tcgccgacgt
     9781 tttccggttc ccgcgcaagg caggcatccg tcccgcgccg gacgcaaact cccgtaacaa
     9841 attgaaggtc agagaaatgc tgcttatccg cgcacaaagc agggtccact tcggtttttt
     9901 gtccgggtcc ggaaacggga cccggagctt tcaaagatcc atagtcggtc agccctcccg
     9961 cacttccaca tgcaggttct gtttcaacgc ccgcgcaatt tcggcgaacg ccgcgttcac
    10021 ctcctgatcc gtcagggtcc ggtccggcga acggaactcc agcgcatagg ctaaactgcg
    10081 cttaccttta ccgatttctt ttgattgaaa gatatcaaaa agcccgacat cggtcaattc
    10141 cttggggccc gcacggcgta tggtctcgac cacctcgcgg tgcgtcaccg cagcgtccgc
    10201 cacaaacgcg atgtcgcgtc gcaccgccgg atactgcggc accggctgaa tcgctgccgc
    10261 cgcaccagcg ccggccagca acggcgccaa ccgcagctca gccagcggca tcggcgcggt
    10321 catgcgccac gcgtgccgca acgccgccga cagcaacccc atgcatccga cttgttcgcc
    10381 gttgagttca attccaaccg cccaccccgt ttccatcgcg ggatgctcgc cgcgcacaaa
    10441 ggtcaggtcg ccaacatgca gggccgccgc gagattctcc accgcccctt tcagccacaa
    10501 cagcgcctcc tcgttaccga ccggacgccg tcgttccacc gacgaccgcc cgaacggtcc
    10561 catcagcccc aacgccacgc gctcttcctc gccgggtacg ccgtttgcct ctttgaaaaa
    10621 gacacgcccc atctcaaaca gcgccgcgca ctcgacttga cgcgaagcgt tgcgcccgag
    10681 cgagcccgcg agctgcggca gcaacgtatc gcgcaacaca ccgtaatcgg cgctcaccgg
    10741 gttcggcagc accacccgcg acgccgcgtg gcgcgtgtca aacgcgtcca actcctgtgc
    10801 cgaaagaaaa ctgtagtgca tcgcctcgct aaaccccatg cccaacagga tctgacggca
    10861 acgcgaccgt gcacggaacg ccccctcatc cagcgtggag acagccgtcg cgtccggcag
    10921 gcgatccgga atcgcgtcca gcccgtgcaa gcgcgcgacc tcttcgatca gatccgcctc
    10981 cagcgtcaag tccagccgcc acgacggaat acggaaagtc gcgccctgcg catcacgcgc
    11041 gatttcttcg agccccaagg acgtcagcag acccaccatc tcgtcgtccg ccatcgccac
    11101 gccgatcact tcacgcgccc gcgcgaagcg caacgtaacc tcgcccgcct tccagccgcg
    11161 gttgtccacg tcgatcacgc ctcgggccac acgcgcctgt ccgtgttcgg ccaacagggc
    11221 caccgccctg cggctggccc aatccgcaag atccggatcc acgccgcgct caaagcggcg
    11281 cgacgactcc gtgccgatcc ccaaccgtgt ggcggtgaat tttgttgccg ccggatcaaa
    11341 caacgcgctt tcgagcaaca catgctcgga gtcggttacg atctcgctgc ccgcgccgcc
    11401 catgacaccc gccaccgcgc tcggcgcctt ggcgtccgca atcaccagca tgctgggatc
    11461 aagcgtgcgc ttcacaccgt ccagcgtcgt gatcgtctcg cccggcgtgg cgcaccgcac
    11521 caccaccgtg tgatccgcca gcgtacggta gtcgaacgca tgcagcggct gaccgcactc
    11581 cagcatcacg tagttggtca catccaccgt caggctgatc gcccgcacgc cgcacaactc
    11641 cagccgatgc tgcatccagg ctggcgaggc cccgtcacgc acgccagtca tcacacgcgc
    11701 cgtgtagcgc ggacacttga ccggatcctc cacacgcacc ttcgcgaaac tctccaccgg
    11761 ctcttctgtc tcggcaaaat ccaccggggg tagacgcaac ggccgccgca acagcgcagc
    11821 gaactcacgc gcgataccga taatcgagag acagtcggga cggttccagg tgatctccag
    11881 atccagcacc gtctcgggtt gcggcaggac atcccggagc ggggttcccg gtttcagcgc
    11941 cgcgtccaac tccaagatgc cagcgtgatc atccgagagc ttcagttcgc gcgccgagca
    12001 gagcatgccg aacgactcca caccgcgcag cttggccttc ttgatcgtga acccgccttc
    12061 gggaatcacc gcgccgatgc gcgcgaaggc cactttcaga ccggcacgca cgttcggcgc
    12121 cccgcacacc acctgccatt cctgttcacc gtccgcaacc gtgcagacat gcagatgatc
    12181 cgaattcgcg tgcgccgcgc aagtcagcac ctcgcccacc acaaaggcgt catcaagtat
    12241 cacgccgacc tcctcgacgc cctccacctc cacgcccgag aacgtcagct tatcggccaa
    12301 ctccctgacc gacaaatccg acacgtccac aaattcattc aaccaactga ccggcacttt
    12361 catacccgca aacccctctt tcgctcaaaa tgaggaaaaa agaataacat aatccgcaac
    12421 agagaccagc cagagaatct cacactgtct cacaaggccg attacgcctg atccgtcgag
    12481 gttgacttgg atgtccagca tcgcctatac tctctcctga ccgcagcagc tgaggcccgt
    12541 gtggaaacgg gagcgttccg gacgttagtc agcggggcgg tctttttgtt tttcgatcca
    12601 catggttttg agcgtgatgc ggggggcgct cttgccagcg gtgacgcgga agctgacgcg
    12661 ctcgcccgtg tcggccacga aggtcacggc gtcgccctca tacgatcctt tttcctgcgg
    12721 cgtactggcg cgccagcggc cggggccggc cacgatccgg cacgcccgga tcatccccac
    12781 gcgtgtgggg agaagatatg ggcgtcttcg attcccgtga ctgtcacctt catgttgcgt
    12841 tcctttcgtc cttttgctac gttgtctttg cgtggccgcc cggtggcctc cgtcggcgat
    12901 gaccgggcga gttgatcggg aggggcggg
//
LOCUS       DAOSRB010000068        12869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4886_length_12869_cov_6.293975, whole genome shotgun sequence.
ACCESSION   DAOSRB010000068 DAOSRB010000000
VERSION     DAOSRB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12869
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4886_length_12869_cov_6.293975"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PLZ74_05245"
     CDS             <1..1007
                     /locus_tag="PLZ74_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97761.1"
                     /translation="VPIAGVEYSGTLNVDGGTMTVCNPEAYVLQIATLAGKGSSSDLY
                     FGQTGWDRTYRGQRVVFDALKSDGTGLESNRVYFVADINTASTIQKTCFSVETNLTLT
                     KTGVTGTPYNSGTGNNIYHTFPAQLRVGNWMTFKTGTLLNTGTLNLASGSLTADELIA
                     TNGTASILSFAGGTLTVKQFTDINNGFATVFGDGESSALLELVAGTHHFADGLVITNN
                     ATLAIGGTNAIGSVGLMGGLAFARGAALDVDFSAATNDWLEVGGAVALADEVTLTLRM
                     SDDTARSPVTILKASEGIEGSAGAWPSATVNNIKYRAVVVGDELRLEKVAQGTMVNIL
                     "
     gene            1036..1971
                     /locus_tag="PLZ74_05250"
     CDS             1036..1971
                     /locus_tag="PLZ74_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOR97762.1"
                     /translation="MKTAALTAAGGVVSAARAHEGGKGAKVCLHLFSKPLQWLGVDRL
                     VEVVAEAGYGGIDLTVRPGGHVKPEKAGTELPRVVEAARRQGLKIRMMTTSLTDPEDP
                     AAERLLKTAAQCGIRIYRMGYLRYAGGRPMGEELERLRRVLAAFEQLNRSCGISGGYQ
                     NHRAWEERLFGGVLWDLHAALKGLDPQWIGSQYDVHHAVAETGGSWVAGMRLIAPHIT
                     STCLKDYIWPAADKQRHQGVFAGEGLVPWKRYYELVKELRIGGPASVHCEWELFTKAE
                     QALTEPERCAIAVRKLKRDSEFFTAQYANCGLTVE"
     gene            2205..3500
                     /locus_tag="PLZ74_05255"
     CDS             2205..3500
                     /locus_tag="PLZ74_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine-DL-glutamate epimerase"
                     /protein_id="HOR97763.1"
                     /translation="MKKTITVADTGCEFEREPLLRPFGFKGGYLTNIWQSAAMVQSTS
                     GQRGIGLCTHSTLWSDAAVFAANSEAGGNALMFAMTAHALRVLKGRSFTDPVALNDEL
                     FKEVYAYGKAITGNPRLRQTFALNALVGVDNAIWLLYAKENGITTFDGLIPAAYRPAL
                     AHRHPKAASIPLMAYAIPISEIKAAVADGSFFMKIKIGQPGTQEEMLAKDMARISEIH
                     KALDGCRTPYTKSGKLPYYFDANGRYEKKETLLRLIDHLKKIGAYDQVAVVEEPFDEQ
                     YECDVHDIPLRLVADESAHTERDAVTRIEMGYRAIALKAIAKTLSMTMKIAQAAHERN
                     VPCFCADLTVNPILVEWNKNVAARLGSFPGLEGLGLVETNGHQNYKNWTVMRSYHPYP
                     DAPWSKTDRGVFHLDADYYAKSGGIFAPLPHYEAMFPKT"
     gene            3517..5235
                     /locus_tag="PLZ74_05260"
     CDS             3517..5235
                     /locus_tag="PLZ74_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97764.1"
                     /translation="MTGGVRCGGGIGIALALSVTMGWGAPQTSLTAGRMRVAATADRI
                     SVSLEGVERFAFPARVAVDTEEGGADRDLFEPAFRQEAGRCVWTSRTTNWEKKEVTLS
                     VEGGAAVLRVTVQGKGKLGKLRFFPQTDRTGPLAYEVSRYLLPVALGGSRCVPQWRNT
                     MESAGIELGYMTPPLLAFPFVGTFRGSCAVGLAPKPGAYHVDHFRCEFAAFREGLFST
                     DFLGYTEVQGEYELPALIMTAGEDAFDALSAYSEWLYREGGCRRIERTQVPRWWLGPF
                     FCGWGEQGYHSPTNVYAGANQKAYAAMSERLDAFGLKPSAIIVDDKWQRRYGELLPDP
                     VKWPDMRAFVEAEHAKGRRVLLWLKAWDNEGLSSEECVKCLCTPYGADPTSPTYRRRI
                     QETMRRLLSAEPGCFNCDGFKIDFANCMPLGRNLATHERNVYGIELLKRLMTLFYESA
                     KAVKPDCLINNSCCHPYFAEVTDQCRLHDYNGQLRSLWEVREFRARLFKTAFPGISVD
                     TDDPHSTSHAEMMNYLRRAPDLGVPDLYQLHRRIRDEDLREIAGIWEAYSARLEKSVD
                     SKGGKR"
     gene            5280..9002
                     /locus_tag="PLZ74_05265"
     CDS             5280..9002
                     /locus_tag="PLZ74_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOR97765.1"
                     /translation="MFRVLLAGFCTAASLRAAEPAVVWVSQPVAPGEAVVVYGGPWTN
                     VTGVMLKGPSELTVTPVRATDDCVTFVYPKTWPLAAFDARVVGGNGTAELRVNAPEVW
                     WVQGDAGMRATPGGWLRVFGRCIGYDGKASLTLRDHTGKATTLAAGESGPYALRVDLP
                     QSLQAGTFEITLRNGLDSRAVPVGAVEILAPREPWPERVFDITAYGAVANDFNDDTAA
                     IRAALAALEQAGGGVLYVPRGRFGIQGELSLPPKTLLRGAGMALSQLYWLDEDNPVGA
                     LVSGTRDFGIEEIFLAAGNIDQGILVVRPKEGETWINENILLRRVRTRFLHTDTVPHE
                     ESFRRLSGGQSFSINGTYVRVIGCDFYSSKGSSGLGGRYLEVRDNRFDGESCGYTGGR
                     FAIFENNNHERQSMSFGNGSRCVYFKGNQLGGIYGNGDRETFTFDGGDMGYIDALQGV
                     GPASVTLKPKPLRRAAERWIDEPLYIAGGRGAGQLRYIRSIAGQEVKLDRPWDILPAT
                     NVLAVIAPVRHRLLILENQVRDGNPFAMYGSGAEMVLGGNKMMRNSGLHAHGMWKGVS
                     EPCWFVEFVENELLEGNAVRGPFSFQVPAADGWIGFFDRGIARPYSYPQNRVGIMRRN
                     VLHNNAYLASHGRVKNLLVENNLIRDADRGIVIAESVEDAVLLRNRFERVLRPYAVGG
                     QTRIGAADSLNAGLSAVEAELGSAVPEAWPVYRKEAEALAAMGLPQEEEAKWVSVMLL
                     RAVRALSESLRGRALSPATVASLMGIDLAQKSIWQFTRVAPGNPRKFAVGGPLLPQWG
                     LPAALTGSAGTFEGWTVRVDTPKPLEPGRPVVCDLYLASTAGPLPPFTLPVRYTLVGQ
                     GWTLAFEERYGWDQLPIQSFLAAGPFANVSGKAIDTTVHPPEIELDMKASYMTRDGNR
                     PWVPVTPNAKGEVDLSQVFKSNELATAHLVAVVRAARGIRVNVPPGGHNALLFVNGER
                     IGSSARRDAPRCVDLRQGDNLFHLISSHTSGAWPIRLTLTMIDPVRPGDLTVVPTDQI
                     ASSPALTASANAVPEGKGLPDSMGVDWKLVVRDDFNRMRLGDQWRGLAVSWMPQGVNI
                     LEGAMRPEFGFGSVLFHREVALPVRIEWKVRRDGPDSKWTGVSLAPKGLSYRTFWYTL
                     QGRGYFLSLGWHDAKNNRVLRNTETVVLDEKGPMLEPGVWHHVVAQFVPPHCRMYLDG
                     KQVLAFEDPDFLPGLNEIGLYSNGHAFDDIRIYTGR"
     gene            9086..12772
                     /locus_tag="PLZ74_05270"
     CDS             9086..12772
                     /locus_tag="PLZ74_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOR97766.1"
                     /translation="MRWMRLGHAAAGFCAAISVGAGEPAVVWLSQPVAPGEAVMVYGG
                     PWTNVTAVTLDGPKQESVVPLKVTDDCVTFEYPHAWPLAAFDVRIRGGNGEAVQRVNA
                     PDVWWVQGDAGKNVTAGGWLRVFGRCIGYDGKAVLEWRDSAGTSRSLPTKSGDRYAVR
                     VDIAPAFPAGVYEVSLLNGLDSRPFRIGKVEVVARRETWPERVFDITAYGAVANDFND
                     DTAAIRAALAALEQAGGGVLYVPRGRFGVRGELSLPPKTLLRGAGMALSQLYWLDEDN
                     PVGALVSGTRDFGIEEIFLVAGNIDGGIVAKRPQAGESWTNENILLRRVRTRFLHTDT
                     TPIAVSYHRSRSSAKPLFVNGTFVRIQECDFYFSKGNSALHGQYLDIRDNRFVGPECG
                     YLSGKQVIFEGNDHEYRGLSFGNGSHNVFFKSNRLGGAYGEGDRETFTFDGGDPAYSD
                     KAVACDGRYVTLKPGSWRHGEAHWIGVPLYIIGGRGAGQMRWVTGIRERTVELDRPWD
                     IQPDDQSHFVLAQMRSQLLFLDNTDRDGNPFALYGTASDVVIAGNSLERTGGLHAHGM
                     FKSVPEPSWFVQFFGNRIVEGNSVRGPFSYVVPASDSWLGFFDRGIRRPLTYPQNRVG
                     VMRGNTLENNAFLAAHGRVKNMLMENNLVREADCGIVIESRVQDAIVRGNRFENVLRP
                     LVLHDQVLCRASERLVAGLSAVDALASDVVPSAAWRELADAAVSLSRRRLPDAEEVTE
                     TRTLLARAASLLAARVGDRKLSAAVVDALFGLDLTQKSDALFSRLQSGKEVKVPLGGT
                     YPVWAVPATLSAAVEGFEGWGVRVASPVKLVPGGQCGFALHVTRPDDRPQTFALPVRF
                     TLDGEGWSVTFTETYRQDTLNVTDFLVAGPFRNASGKPIDSAVHPPESRLDVTESYDT
                     LDGRKPWRAVRAAKPDGTVDLNTCFRNTELATAHAVAIVYAARPLRVRMQYGSNQNTL
                     ARVNGEAVGSSARRDAYNGRPVDLREGENVIHLISTHATGAWPLKVRLTAVDATRPGE
                     LRVVSSDRLFQSDRLKAEGRRGVVGEPFTDGEGRVWRLSHEDTFNRLRVGLQYEGRDP
                     IEWRDHSVRILDGRLVGAGRITLNLRLSAPLRVEYDVPVVNGRANTGLTLAARGASAV
                     VRDWGEAMKQKQVVTLNNVLGGLTGETARVVAVFDTKGSTLFISGKKMREVPDTEWVR
                     GLDELTLSGTLDNLRIHTGERGKP"
BASE COUNT         2342 a         3457 c         4600 g         2470 t
ORIGIN      
        1 atgtgccgat cgccggagtg gaatattcgg gaacgctaaa cgtggacggc gggaccatga
       61 ccgtgtgcaa tccggaggcg tatgtgctcc agatcgcgac acttgcaggc aaggggtcaa
      121 gctccgacct ctatttcggt cagacgggat gggaccgtac ctatcgcggt cagcgggtgg
      181 tgtttgacgc gctgaaatcc gacggcaccg gtttggagtc aaaccgcgtg tattttgtgg
      241 cggacatcaa taccgcctct acgattcaga agacctgctt cagcgtcgag accaatctga
      301 cgctcactaa aaccggtgtc acgggaacac cgtacaattc aggcacgggc aacaatatct
      361 atcatacatt cccggcgcag ttgcgtgtcg gcaactggat gacgttcaag accggaaccc
      421 tgcttaatac cggcacgctg aatctggcga gcggttcgct gacggcggac gagctgatcg
      481 ccaccaacgg cacggcgagc atcctgagtt ttgcgggcgg tacgctgacg gtcaagcagt
      541 ttaccgacat caacaacggt ttcgcgacgg tcttcggcga cggcgagtcg tcggcgctgc
      601 ttgagctggt tgccggcacg caccactttg ccgacggtct ggtgatcacg aacaacgcca
      661 cgctggcgat cggcgggacc aatgcgatcg gctctgtagg cttgatgggt ggcctcgcct
      721 ttgcgcgcgg ggcggcactc gatgtggact tcagcgcggc gaccaacgac tggctggagg
      781 tggggggcgc tgtggctctg gcggatgagg tgaccctgac cttgcgcatg tcggatgata
      841 cggcgcgttc gccggtgacg atcctgaagg cgtccgaagg gatcgagggt tcggctggcg
      901 catggccgtc tgccacggtc aacaacatta agtaccgggc ggttgtggtt ggcgatgagt
      961 tgaggcttga gaaggtcgcg cagggcacga tggtgaatat cctgtagggg aggcggaatg
     1021 cggcgcaggg attttatgaa gacagcggcg ctgacagcgg ccgggggggt ggtttctgct
     1081 gcgcgtgcgc acgagggcgg gaagggcgcg aaggtgtgtt tgcatctctt ctcaaagccg
     1141 ctccaatggc tgggggtgga ccggctggtt gaagtggtgg ctgaggccgg ttatggcggg
     1201 atcgacctga cagtgaggcc gggtggtcat gtgaaacccg aaaaggccgg gacggaactg
     1261 ccccgcgtag tggaggccgc gcgcaggcag gggctcaaga tcaggatgat gaccacctcc
     1321 ctgaccgacc cggaagatcc ggcggccgag cgtttgctga agacggcggc acaatgcggg
     1381 atccggattt accgcatggg gtatttgcgt tatgccggcg ggcggccgat gggggaggag
     1441 ttggagcggt tgcgtcgcgt gttggcggct ttcgagcaac tgaaccggtc atgcgggatc
     1501 agcggtggtt accagaatca ccgcgcgtgg gaggaacgct tgttcggcgg cgtattgtgg
     1561 gacctgcacg cggcgctcaa ggggcttgac ccgcaatgga tcggtagcca atatgatgtg
     1621 catcatgcgg tggcggagac gggcgggtcg tgggtggcgg ggatgcggct catcgcgccg
     1681 cacatcacga gtacctgtct caaggactat atctggccag cggccgacaa gcaacgtcat
     1741 caaggcgtgt tcgcaggcga ggggctggtg ccgtggaagc ggtattatga actggtgaag
     1801 gaactccgga ttggggggcc ggcatcggtt cactgtgagt gggagctgtt cacaaaagcg
     1861 gagcaggcgt tgacggaacc ggaacggtgc gcgatcgcgg tgcgcaagct gaagcgcgac
     1921 agcgagttct ttacggcgca gtatgcgaat tgcgggctga cggtggaatg agccacggat
     1981 ggggtacgga tggaacgcgg atttggagtg ccggatcaac gtgttgttca aaggcgttct
     2041 cgtgggcaag ggttatgcgg aacaatccgc gttccatccg tgtccataac aacagaaagg
     2101 atgtcatgaa acgcagggat tttatgaagg cggcggcact gacggcggga gtaacactgg
     2161 caaacggatg tgtctcggcg ggcgcgtcaa acggagaaaa gacgatgaaa aaaacgatca
     2221 ccgtggcgga cacggggtgc gagttcgagc gcgagccgct actgcggcct ttcggattca
     2281 agggggggta cctgaccaac atctggcaaa gcgccgcgat ggtgcaaagc acatccggtc
     2341 agcgtgggat cgggctctgc acacacagca ccttgtggag cgatgccgcg gtgtttgcgg
     2401 cgaattccga ggcgggcggg aacgcgctga tgttcgccat gaccgcgcac gcgctacgcg
     2461 tgttgaaggg acggtcgttc accgatcccg tggcgctcaa cgacgagctt ttcaaagagg
     2521 tgtacgcata cggcaaggcc atcaccggca acccccggtt gcgtcagacc tttgcgttga
     2581 acgcgctggt cggcgtggac aacgcgattt ggctgctcta cgcgaaagaa aacgggatca
     2641 cgacgtttga cggactgatc ccggcggcat accggcccgc gctggcccac cgccatccca
     2701 aggcggcctc gattccgctg atggcctatg cgattccgat cagcgagatc aaggcggcgg
     2761 tggcggacgg ttccttcttc atgaaaatca agatcggtca gcccggcaca caggaggaga
     2821 tgctggccaa ggacatggcg cggatcagtg agatccacaa ggcgctggac ggttgccgca
     2881 caccttacac gaagagcggc aaactgccgt actatttcga cgccaacggt cggtacgaga
     2941 aaaaggaaac gctgctgcgc ctgatcgatc atctcaagaa gatcggggcg tacgatcaag
     3001 ttgcggtggt ggaagagccg tttgacgagc aatacgagtg cgatgtccac gatattccgc
     3061 tgcggctggt cgcggacgag agcgcgcata cggaacggga cgcggtcacg cgcatcgaga
     3121 tgggctatcg ggccatcgcg ctgaaggcga tcgccaagac gctcagcatg acgatgaaaa
     3181 tcgcgcaggc ggcacacgag cggaatgtgc cgtgcttctg cgcggacctg acggtgaacc
     3241 cgatcctcgt ggagtggaat aaaaacgtgg cggcgcggct cggttcgttc ccgggactgg
     3301 aaggcctcgg actggtggaa accaacggcc atcagaacta taagaactgg acggtcatgc
     3361 gcagctatca cccgtatccg gacgcgcctt ggtccaaaac cgaccgcggc gtgtttcatc
     3421 ttgatgcgga ctattacgcg aagagcggcg ggattttcgc gcccctgccg cactacgagg
     3481 cgatgttccc caaaacgtga cggaaggaag tgtggaatga cgggtggggt tagatgtggg
     3541 ggagggatcg ggatagcgct cgcgctgtct gtgacaatgg ggtggggagc gccgcaaacc
     3601 tcgctgacgg cgggacggat gcgggtggct gcgacggccg accggatttc cgtttccctg
     3661 gagggggtgg aacggttcgc gtttcccgcg cgcgtggcgg tggatacgga agagggcggg
     3721 gcggaccggg atctgtttga gcccgccttc cggcaggagg cggggcgctg cgtgtggacg
     3781 tcgcgcacaa ccaactggga aaagaaagag gtgacgcttt cggttgaggg aggcgcggca
     3841 gtgctacgcg tcaccgtgca gggcaagggg aaactgggaa agcttcgctt tttcccccag
     3901 accgatcgga ccggtccgct cgcctacgag gtgtcgcgct atctgttgcc cgtcgccttg
     3961 ggcggttccc ggtgcgtgcc gcagtggcgc aacacgatgg agagcgccgg gatcgagtta
     4021 gggtacatga cgccgcccct gttggcgttc ccgttcgtcg gaacgttccg gggtagttgc
     4081 gcggtcgggc tggctcccaa gcccggcgcg tatcacgtcg accatttccg ctgcgagttt
     4141 gccgcattcc gggaggggtt gttctccacg gactttctcg gttatacgga ggtgcagggg
     4201 gagtatgagc ttcccgcatt gatcatgacg gctggcgagg atgcgttcga tgcgttgtca
     4261 gcctactccg agtggcttta ccgtgaaggc gggtgccgca ggatagagcg gacgcaggtg
     4321 ccgcgctggt ggctggggcc gttcttctgc ggttggggag agcaggggta ccattcgcca
     4381 acgaatgtgt acgcgggtgc caatcaaaag gcgtatgccg caatgtccga acggctcgac
     4441 gcctttggtc tgaaaccgag cgcgatcatt gtggatgaca agtggcagag gcggtatggc
     4501 gagctgttgc ccgatccggt gaagtggccg gacatgcggg ctttcgttga ggcagagcac
     4561 gccaagggcc gcagggtgct gctttggctc aaggcatggg acaacgaagg gctctcttcc
     4621 gaagagtgcg tgaaatgcct gtgcacgccg tacggagcgg atccgacttc gccgacctac
     4681 cgcagacgca ttcaggaaac catgcgccgc ctgctctcgg cggaacccgg ttgctttaac
     4741 tgcgacgggt ttaaaatcga cttcgccaac tgcatgccgc tgggacggaa tctggccacg
     4801 cacgaacgaa acgtctacgg catcgaactg ctgaagcggc tgatgacgct gttctacgag
     4861 tctgccaagg cggtcaagcc cgactgcctg atcaacaaca gttgttgcca tccctatttc
     4921 gccgaggtca ccgaccagtg ccgcctgcac gactacaacg ggcaactgcg gagtttgtgg
     4981 gaagtccgcg aattccgggc gcggcttttt aaaaccgctt ttccggggat cagcgtggac
     5041 acggatgatc cgcactccac gtcacacgcc gagatgatga attacctgcg gcgcgcgccg
     5101 gatctgggtg tgccggatct ctatcaattg caccggcgca tccgggacga ggatctgcgg
     5161 gagatcgccg ggatttggga agcgtattcc gcgcgtttgg agaagtccgt cgattcgaag
     5221 ggcgggaagc ggtgaaaaaa ccgtaaaaat caaaaggggg ggcgggaatg ggtaagggca
     5281 tgtttcgtgt actcttggcg gggttctgta cggcggcgtc gcttcgggcc gccgagccgg
     5341 ccgtggtgtg ggtttcccag ccggtggcgc cgggagaggc ggtggtggtt tacggcgggc
     5401 cgtggacgaa cgtgacgggt gtgatgttga aggggccctc ggaactgacg gtcacgcccg
     5461 tgcgggccac ggacgattgt gtgacgttcg tgtatcctaa aacgtggccg ctggcggcat
     5521 tcgatgcgcg ggtagtgggc gggaacggaa cggcggaatt gcgggtgaac gcgcctgagg
     5581 tgtggtgggt gcagggagat gccggcatgc gggcgacacc cggcggatgg ttgcgtgtgt
     5641 tcggccgctg tatcggctac gatggcaagg cctctctgac gcttcgcgac catacgggaa
     5701 aagcaaccac attggctgcg ggagaaagcg gcccctatgc cctgcgtgtc gaccttcccc
     5761 agagtcttca ggccggtacg ttcgagatca ccctccggaa cggactggac agtcgtgcgg
     5821 tgccggtcgg ggcggtcgag attctcgcgc cgcgcgagcc ttggccggaa cgggtgtttg
     5881 acatcaccgc gtacggcgcg gtggcgaatg atttcaatga cgataccgcc gcgatccggg
     5941 ccgcgctggc tgcgctggag caggccggcg gcggtgtgct gtacgtgccg cgcggccgtt
     6001 tcgggataca gggcgagttg tcgctgccgc ccaagaccct gttgcgcggg gcgggcatgg
     6061 cgctcagcca actctactgg ctggacgagg acaatccggt tggcgcactg gtgagcggca
     6121 cgcgggattt cgggatcgag gagatttttc tggccgcagg caacatcgac caaggcattc
     6181 tggtggtccg cccgaaagag ggtgagacct ggatcaacga gaatatcctg ctgcgccgcg
     6241 tgcgcacacg gtttttgcac accgacaccg tgccgcacga ggagtccttc cgtcgcttga
     6301 gcggcgggca gtcttttagc attaacggga cgtatgtgcg ggtcatcggg tgtgacttct
     6361 actcgtccaa aggatcgtca ggactgggag gccgttatct cgaggtgcgg gataaccggt
     6421 ttgacggcga gagttgcggt tacactggcg gacggttcgc gatctttgag aacaacaacc
     6481 atgagcgcca gagcatgagt ttcggcaacg gcagccgttg cgtctatttc aaaggcaacc
     6541 aactcggcgg gatctacggc aacggggatc gcgagacctt taccttcgat ggcggtgaca
     6601 tggggtatat cgacgcgctt cagggggtcg gcccggcgag tgtcacgctc aagcccaagc
     6661 cgctgcgccg cgctgcggaa cggtggattg acgagcccct gtacattgcc ggcgggcggg
     6721 gtgcgggcca gttgcggtac atccgctcca tcgcggggca ggaggtcaag cttgaccggc
     6781 cgtgggatat actgccggcc acgaacgtgc tcgcggttat cgcgccggtg cgccaccgtc
     6841 tgctgattct cgaaaaccag gtgcgtgacg gcaatccctt cgccatgtac ggcagcggtg
     6901 cggagatggt gttgggcggt aacaagatga tgcgtaacag cgggctgcat gcgcacggca
     6961 tgtggaaagg cgtttccgag ccctgttggt tcgttgagtt tgttgagaac gaattgttgg
     7021 agggcaatgc ggtgcggggt cccttctcgt ttcaggtgcc ggccgctgat ggctggatcg
     7081 ggtttttcga ccggggcatc gcgcggccgt attcctatcc gcaaaaccgg gtcggcatca
     7141 tgcgccgcaa cgtgctccac aataacgcgt atctggccag ccacggccgc gttaaaaacc
     7201 tgctggtgga gaacaacctg atccgggacg cggaccgggg gatcgtgatc gccgaaagtg
     7261 tggaggatgc ggttttgctg cgcaaccggt tcgagcgggt cctgcgcccg tatgcggttg
     7321 gcggccagac gcggatcgga gcggctgaca gcctgaacgc cggattgtcg gcggtggagg
     7381 cggagttggg cagcgcggtg ccggaggcgt ggcccgtcta ccgtaaggag gccgaggccc
     7441 ttgcggcgat gggtctgccg caagaagagg aggcgaaatg ggtctccgtg atgttgttac
     7501 gcgcggtgcg ggcgttgtcg gaaagcctgc ggggcagggc gctctccccc gcgacggtgg
     7561 cgtcgctgat ggggatcgat ctcgcccaaa aatcgatttg gcagttcacc cgggtggccc
     7621 cgggcaaccc gaggaaattc gctgtgggcg gtccgctcct gccgcaatgg ggtttgcctg
     7681 ccgcactgac cggaagtgcc gggacgtttg aaggttggac ggtccgggtc gatacgccga
     7741 agccgctgga acccggcagg ccggtggtct gcgatctgta cctggcgagc accgccgggc
     7801 cgttgccgcc cttcacgctg ccggtccgat acacgcttgt cggccaagga tggacgctcg
     7861 cgttcgagga gcgttacggt tgggatcagc ttccgatcca gtcttttttg gctgcgggac
     7921 cgtttgccaa cgtgagcggc aaggcgatcg acacgacggt gcatccgcca gaaatcgagt
     7981 tggacatgaa ggcgtcgtac atgacgcgtg acgggaatcg cccgtgggtg ccggtcacgc
     8041 ccaatgccaa gggcgaggtg gatctctcgc aggtgttcaa gtccaacgaa ctcgccacgg
     8101 cgcatctggt ggcagtggtg cgcgcggcgc gcgggatacg cgtgaatgtg ccgccgggag
     8161 ggcacaacgc attgctcttc gtcaacggcg aacgcattgg gtccagcgca cggcgtgacg
     8221 cgccgcgctg tgtggatctg cgtcaagggg acaatctttt ccacctcatc tcgtcgcaca
     8281 cctcgggtgc gtggccgatc cgcctcaccc tcaccatgat cgatccggtg cgtcccggtg
     8341 atctgacggt tgtgccgacc gatcagatcg cgtcgtcgcc cgcgctgacc gcaagcgcga
     8401 acgccgtgcc ggagggcaag ggattgccgg attcaatggg cgtcgattgg aaactcgtgg
     8461 tccgggacga ttttaaccgg atgcggctag gggatcagtg gcgcgggctc gccgtcagtt
     8521 ggatgccgca gggcgtgaac attctggaag gcgcgatgcg tccggagttc gggttcggtt
     8581 cggtgctttt ccatcgcgag gtcgcgttgc cggtgcggat cgagtggaag gtccgccggg
     8641 acggcccgga ttccaagtgg accggtgtgt cgctggcacc gaaggggctc tcgtaccgga
     8701 cgttctggta cacccttcag ggccgcgggt attttctgtc gcttggatgg cacgacgcga
     8761 agaacaatcg ggttctgcgg aataccgaga cggtggtgct ggatgagaaa ggcccgatgc
     8821 tcgagcccgg ggtctggcat catgtggtgg cgcagttcgt gccgccgcac tgccgcatgt
     8881 atctggacgg caaacaggtg ttggcgttcg aggatccgga cttcctgccg gggttgaatg
     8941 aaatcggcct gtacagtaac gggcacgcct ttgacgacat tcgtatctac accggaagat
     9001 gattggggcg gaagaacgaa gtgcggggtg cgaaggaaga tcgctctccg tggcgtgaat
     9061 ggcatgtgat gaaaggggat cggatatgcg ctggatgcgt ttgggtcatg cggcggcggg
     9121 gttctgtgcg gcaatttcgg ttggggccgg agaaccggcg gtggtctggc tgtcgcagcc
     9181 ggtggcgccc ggtgaggcgg tgatggttta cggcggtccg tggaccaacg tgaccgcggt
     9241 gacgctggac ggacccaagc aggaaagcgt ggttccgttg aaggtgacag atgattgcgt
     9301 caccttcgag tatccgcacg cgtggccgct ggcggcgttc gacgtgcgga tccgcggcgg
     9361 taacggcgag gcggtgcagc gggtgaacgc accggacgtt tggtgggtac agggtgatgc
     9421 cgggaaaaac gtgaccgcag gcggttggct gcgtgtgttc ggacgttgta tcggatatga
     9481 tggaaaggcc gtactggaat ggcgggacag tgcggggacg tcgcggagcc tgccgacgaa
     9541 aagcggggac cggtacgcgg tgcgggtcga catcgccccg gcttttccgg ccggcgttta
     9601 tgaggtttcg ctgttgaacg ggctggacag ccgtcctttt cggatcggca aagtcgaagt
     9661 ggtggcgcgg cgcgaaacgt ggccggaacg ggtgtttgac atcaccgcgt acggtgcggt
     9721 ggcgaatgac ttcaatgacg acactgccgc gatccgggcc gcgttggcgg cgttggaaca
     9781 ggcgggcggc ggtgtgctgt acgtgccgcg cggccgtttc ggggtacggg gcgagttgtc
     9841 tctgccgccc aagaccctgc tgcgcggggc gggcatggcg ctcagccaac tctactggct
     9901 ggacgaggac aatccggttg gcgcactggt gagcggcacg cgggatttcg ggatcgagga
     9961 gatttttctg gtcgcaggca atatcgatgg cggcattgtg gcgaaacggc cccaggcagg
    10021 ggagtcatgg accaacgaaa acattttgct gcgccgtgtc cgcacgcggt ttctgcacac
    10081 ggacaccacg ccgattgcgg tcagttacca tcgttcccgt tcgagtgcga aaccgctgtt
    10141 tgtcaacggg acgttcgtcc ggattcagga gtgtgacttt tatttttcaa aagggaactc
    10201 tgctttgcat ggccaatatt tggacatccg cgacaatcgg tttgtggggc cggagtgcgg
    10261 atatctgagc ggtaaacagg tcatttttga ggggaatgac catgagtacc gtggcctgag
    10321 ctttgggaat ggcagtcaca acgtgttttt caagagcaac cggttgggcg gcgcgtacgg
    10381 ggagggcgac cgcgagacgt tcacctttga cggaggggat cccgcctaca gtgacaaggc
    10441 cgtggcgtgt gacggaaggt atgtgacgct gaagcctggt tcctggcgtc acggcgaagc
    10501 gcattggatc ggcgtaccgc tctacatcat cggtggacgc ggtgcgggac agatgcgctg
    10561 ggtcaccggt atccgggaac gtacggtgga gctggaccgg ccgtgggata tccagcccga
    10621 tgaccagtcc catttcgtgt tggcccagat gcgtagtcag ttgctctttc tcgacaatac
    10681 cgaccgtgat ggtaatccgt tcgccctgta cgggaccgcc agcgacgtgg tcatcgcagg
    10741 taacagcttg gagcggaccg gcggtctgca cgcgcacggc atgttcaaat ctgtgcccga
    10801 gccttcctgg tttgtccagt ttttcgggaa ccgcattgtg gaggggaatt cggtgcgcgg
    10861 ccccttctcg tacgtggtcc cggcgagtga ttcctggctc ggctttttcg accggggtat
    10921 ccgcaggcct ttgacctatc cccagaaccg ggtcggcgtt atgcggggga acacgctgga
    10981 gaacaatgcg tttctggctg cgcacgggcg cgtgaaaaac atgctgatgg aaaacaatct
    11041 ggttcgggaa gcggactgcg gcatcgtgat cgagtcgcgt gtgcaggacg cgatcgtgcg
    11101 gggtaaccgt ttcgaaaacg tcttgcggcc gctggtcttg cacgatcagg ttttatgccg
    11161 tgcctcggaa cggctggtcg ccggactctc cgctgtggac gcgctggcgt ccgacgtggt
    11221 gccgtcggcg gcgtggcggg aacttgccga tgcagcggtt tcgctctcgc gccgcaggct
    11281 gccggatgcg gaagaggtca cggagacgcg gactctgctc gcgcgcgccg cgtcacttct
    11341 tgctgcgcgc gttggcgacc ggaagctctc cgcggccgtc gtcgacgcat tgttcggttt
    11401 ggatctgacg cagaaaagcg acgctctctt ttcgcgtctc cagagtggca aggaggtgaa
    11461 ggttcctttg ggcggcacat atcccgtctg ggcggtgccg gccacgctct ctgcggcggt
    11521 cgagggtttt gaggggtggg gggtacgcgt ggcgtcgccc gtgaaactcg tgcctggggg
    11581 acagtgcggc ttcgccctgc atgtcacccg tccagatgac cggccgcaaa catttgcgtt
    11641 gcccgtccgg ttcacgctcg atggggaggg gtggagcgta acgttcacgg aaacctaccg
    11701 gcaggacacg ctgaatgtga cggatttcct agtcgccggt cccttccgga acgccagcgg
    11761 caagccgatt gacagtgcgg ttcatccgcc ggagagccgg ctggatgtga ccgagtccta
    11821 cgacacgctg gacggccgca agccgtggcg ggccgtgcgt gccgccaagc cggacggaac
    11881 ggttgacctg aacacatgtt tccggaacac ggaactggcc acggcgcatg cggtggccat
    11941 cgtatacgcg gcgcgtcctc tgcgggttcg aatgcagtac ggctccaacc agaacacgct
    12001 ggcgcgggtc aatggcgaag ccgtgggctc cagtgcgcgg cgtgatgcgt acaatggacg
    12061 ccccgtggac ttgcgggagg gggagaatgt gatccacctg atcagcacgc atgccacggg
    12121 tgcctggccc ctgaaagtcc ggctgacggc ggttgacgcg actcgtcccg gcgaactgcg
    12181 ggtggtttcg tccgaccgac ttttccagtc tgaccggctc aaggccgaag ggcggcgcgg
    12241 ggttgtgggc gagcccttca cggatgggga gggacgcgtc tggcggttgt cgcatgagga
    12301 caccttcaac cggctgcgtg tggggttgca gtatgagggg cgggacccga tcgaatggcg
    12361 tgaccattcg gtacgcattc tcgacggtcg attggtgggt gcgggacgga tcacactgaa
    12421 tctgcgcctc tcggctcctc tccgcgtgga gtatgatgtt ccggtcgtga atggccgcgc
    12481 gaacaccggg ctgacattgg ccgcgcgcgg agcatcagct gtggtgcggg attggggaga
    12541 ggccatgaag caaaaacagg tcgtcacgtt gaacaacgtg ctgggtggcc tgaccgggga
    12601 gaccgcgcgg gtggtggccg tgtttgacac aaaagggagc acgctcttta tcagcggaaa
    12661 aaaaatgcgg gaggttcccg acacggagtg ggttcgcggg ttggatgaac tcaccctgag
    12721 cggaacgctg gacaatctgc gcatccatac cggggagagg ggcaagccct gaaagacacg
    12781 gttccgccgg tcggtctgta cgatgcctga ttctgctccc gacagtagcg tttaccgctc
    12841 ccgacagtag cgtttaccgc tcccgacag
//
LOCUS       DAOSRB010000069        12856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4900_length_12856_cov_6.873994, whole genome shotgun sequence.
ACCESSION   DAOSRB010000069 DAOSRB010000000
VERSION     DAOSRB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12856
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4900_length_12856_cov_6.873994"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLZ74_05275"
     CDS             complement(<1..472)
                     /locus_tag="PLZ74_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97767.1"
                     /translation="MIRNKQILSLMLLTAFLAVSARGVYMVHEFYLPLPEAQVLKAFN
                     TLDGLGQVKAPIDSVTSIVVTGPDTIIHYDHWEDGYEVDINNPAQPTTRIWGDGNDAN
                     GIPPGYASDPMGLPQGAVISLRNNVNLPRNPSQILYDGRDRITASKALAVSRALW"
     gene            922..1341
                     /gene="rnk"
                     /locus_tag="PLZ74_05280"
     CDS             922..1341
                     /gene="rnk"
                     /locus_tag="PLZ74_05280"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOR97768.1"
                     /translation="MTKRTLYISEMDRHKLEELVNVAHLSHDRDQGDLKSLQNELQRA
                     LVVEPEQVPPNVVTLHSRVRLREVKTQTVMEVTLVMPSEANVDAGKLSVLSPVGTAIL
                     GYAEGDTIEWMVPAGHRRFVIEKLLYQPEASSRSSRK"
     gene            1352..2392
                     /locus_tag="PLZ74_05285"
     CDS             1352..2392
                     /locus_tag="PLZ74_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOR97769.1"
                     /translation="MTIDGRPVTLKPGMTVYEAAQGIGIRIPVLCHADGVVPAGGCGL
                     CTVEDTAAGGKLLPACATRADAAMSIVTDSEAVRNFRRTALELLLSNHPADCEAPCRM
                     ACPSGLPVQTLMARIRSGDWQSARDLARAHPFACGGAAPCEKACRRALLDGAVAICAL
                     HRWLAGETAGEACSRVPGGTQARPRFRSRMPDLNVAALAALTPETGPRRLPPETHEPT
                     PEAAAFEAGRCMRCGCLKPTKCRLRELCAEYGANPRAFDHAPRPVIRETGRNGFRFDA
                     SRCILCGLCVRITRQCGAGELGPAFHGRGFEARIGPPLGRVWDDLPDSVLQACAAACP
                     TGAMAFETNAVV"
     gene            complement(2399..3382)
                     /locus_tag="PLZ74_05290"
     CDS             complement(2399..3382)
                     /locus_tag="PLZ74_05290"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOR97770.1"
                     /translation="MSARKLTAKITGVGKALPEGRLTNADLEKRVDTNDAWITQRVGI
                     RERRIAAEGEHTSDFAIRAARDALALSGLKPEQIDLILVGTSTPDMIYPSVACQVQQA
                     LGASNAGVLDISAACTGFIYGASLAWGMIQSGALRHVLVIGAEKNSAIVDWSDRDTCI
                     LFGDGAGAAIFSEHRSNTDGVLSAKLGGDGSFGGLLHLPNSGCRSFPEENGRFIRMQG
                     NEVFKQAVRNMYECAVCVLDQAGLSSQEVSLLIPHQANVRIIDAVAKRLGLPKEKVFR
                     NIAEYGNTSAATIPIALCEALEQGRLKKGDVLVIDAFGAGLTWGAMAIRWA"
     gene            complement(3483..5549)
                     /locus_tag="PLZ74_05295"
     CDS             complement(3483..5549)
                     /locus_tag="PLZ74_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97771.1"
                     /translation="MLRSWAFRALLAVLPGFLCAQQPAYIGFVFPAGGRQGTVFRCTL
                     GGQALDGVNGVIFTGSGVTGRVIEYNRHMNSQEVQLLREQLDEIRAMPKARRDAQVTN
                     LASRLEMLIGDYVQQPQCASIANLVTVEVAVERGTPPGPREIRLVTPRGVSNPLAFHV
                     GALPEVTSEPLPTSPKQILGKEAQSLRSTSGRKRGAAGKGRGRRGMVEREAEMIQMVS
                     GMAGRGALTDVDDREVRIMPPCTVNGQIASGSVDRYRFDGRSGQRLVVTVQARALVPY
                     LADAVPGWFQPVLVLCDAQGREKAYNDDYRFHPDPVLYCELSANGEYVLAIHDAIFRG
                     REDFVYRMTIGELPFVTSVFPLGGQAGVDAVIEVKGFHLAETRLTPDLRTRLPGLHTL
                     TVAGSGGLRSNPFVFEISTLPDGMEHEPNNTLKRAQPVTLPIVMNGTIGMPGDLDVFT
                     FRGRAGQEVVAEVRARRLGSPLDAWLKIMDDQGRTLAWNDDQEDPGSGLDTHHADAYV
                     RVTLPADGAYAVQLRDTQHQGGEAFGYRLRLSEPQPDFDLRVVPSRVVVRRGGETPVT
                     AHALRKDGFAGPITFRIREPAAGFEVRGATLTGTQTVARLMIRARQPSDSGVPVPIVI
                     QGVSGEGEGAIIRDAVAAEDCMQAFLWRHLVPAQEFLVLMIPGPPRPSGKPGFPAASR
                     RREKPE"
     gene            complement(5668..6999)
                     /locus_tag="PLZ74_05300"
     CDS             complement(5668..6999)
                     /locus_tag="PLZ74_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOR97772.1"
                     /translation="MRERKSVTRRDLLRTGLCGAAVAVWPGRTVRGQAAPAARKATAK
                     SVIQVFLWGGMSHTDTWDPKPDAGYDYTGAFKDVIPTNVDGIRLGALFPKLAQQADKF
                     SILRSMTHRNNGHETAAYLMQTGHMPGQRLAYPAIGAVFALFKGEAAGYTGLIPPYVV
                     LTQPQGRFSEAGFLGSRCKPFATGGDPNAPRFEVEGVIARGITDQRQEARRGLLSSLN
                     TLGNTLSGHPELAAAREAEKRAYDLILGQGRDVFDLSKEEATIRDRYGRHTFGQDCLA
                     ARRLVEAGVPYITINYPGGWDTHSNHFQTMNRQCPQLDQGLAALLQDLAERGLLESTL
                     VWCCGEFGRTPKVDWQPPWNGGRNHHGNVFSVLVAGGGFRGGHLVGASTAKGEEVEER
                     PIYPIDLLGSIYELAGIDTEAALPHPEGADARVLDMREAGAKSAGRLKEIM"
     gene            complement(7013..8569)
                     /locus_tag="PLZ74_05305"
     CDS             complement(7013..8569)
                     /locus_tag="PLZ74_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOR97773.1"
                     /translation="MWRIVCLIVAVRAGFGAPAPEAALFLQTGTPTPENPVDAPIFRK
                     LKRHGITPVYCTDAVFLRRAYLDILGTLPTADEARAFLADTSMHKRTALIDRLLERDE
                     FADYWAMRWSDLLRVKAEFPVNLWPNAAQAYHRWIRTAMRDNKPYDCFARELLLSNGS
                     NFRVGPVNFYRATPDRSPEGLASAVALTLMGARTEHWPSNTLADLAVFFSQVRYKPTR
                     EWKEEIVFWDPEQPLTTTAGRLPDGTSVRLEPGRDPRTAFAEWLITPQNPWFTRALVN
                     RVWYWLLGRGIIHEPDDIRSDNPPSHPDLLKRLQREFVRSGYDLRKLYRLILTSRTYQ
                     FSPVFPSGRVESAAPLFAYYPLRQLDAEVLIDVINKVTGTSDLYTSAIPEPFTFIPAN
                     KPAVALPDGSITSPFLELFGRPARATGLASERINRALPPQRMHLLNSSHIQRKLETSP
                     VLRRMFRGKRGSRAMIEEAYLTTLSRFPSATEIGTVERYLRSGVVKEDAAGVDLIWAL
                     INSDEFLYRH"
     gene            complement(8677..9945)
                     /gene="tyrS"
                     /locus_tag="PLZ74_05310"
     CDS             complement(8677..9945)
                     /gene="tyrS"
                     /locus_tag="PLZ74_05310"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOR97774.1"
                     /translation="MNILDTFKARHLFEDMTSPGLAKALDKPLTVYAGFDPTSDSLQA
                     GNFVTIMALAHFQRAGHKVIALVGGATGLIGDPSGKSKERNLLSAEQVEHNLVGIREN
                     LSRFLDFNPDSANPAILVNNYDWFKSFTFIDLLRDVGRYFRMGVMLSKDSVKKRMESD
                     DGMSFTEFCYQIMQGYDFYHLHRTFGCTLQIGGSDQWGNITAGTELVRRICGEEVFGM
                     TFPLVCDSTGKKFGKSEGNAIFLDARKTSYYEFYQFFLRTLDADVIRYLKIFTFLPMG
                     RIAELEQAVAAAPEKREAQQVLAEEVTRTVHGENGLAVAQKATGVLFGGAIEGLAAAE
                     LEAIFVNVPSATLPADHVLGKPAFEVIAAAGMVASKGEARRLVQQGGLYINNARVTGI
                     ERVFDASDLCEGRLTVLRSGKKSFFLLKVR"
     gene            complement(10080..11765)
                     /gene="rpsA"
                     /locus_tag="PLZ74_05315"
     CDS             complement(10080..11765)
                     /gene="rpsA"
                     /locus_tag="PLZ74_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOR97775.1"
                     /translation="MMAAALDQQMNQFVAGSIIKGTISGIKGNEVLVDIGYKSEGIVP
                     ANEFEDVTQLKIGDEIDVLLMELESESGMVSLSKARADEKLRWDAVLAKYSEGGVVTG
                     TIRTKVRGGLIVDVDGVDAFLPGSQVDVTPVHDPDPYLNQSFEFKVIKISNERRNIIV
                     SRRELIEERMADKKRELLGTIEKGQMRHGHVKNITDFGAFVDLNGLDGLLHITDMSWG
                     RIKHPSEMLKVGQELDVLILDVDMERERVSLGLKQATPNPWENIEGTFPVGSRLRGKV
                     VNLVPYGAFVEIQPGIEGLVHVSEFSWTKRVARASDVLNVGDEVDVVVLSIDAANQKI
                     ALGIRQTEANPWDTVQDRYPVGSRVTGKVRNFTTYGAFVELEEGIDGMIHVSDMSWTR
                     KINHPSEVLQKGQQVEAVVLEVNPADQRISLGLKQASDDPWNTITSRYAIGQIVKGKV
                     SKIASFGAFVELEDGVDGLVHISQISDQHVEKVKDALKVGQDVEARIVKIDRDERRIG
                     LSIKAVSMDESEIAELTRDLSTVDLKPGENLVGLAAAFDDAFAQSEEWQPGAE"
     gene            12213..12289
                     /locus_tag="PLZ74_05320"
     tRNA            12213..12289
                     /locus_tag="PLZ74_05320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12247..12249,aa:Pro,seq:tgg)
     gene            complement(12529..>12856)
                     /locus_tag="PLZ74_05325"
     CDS             complement(12529..>12856)
                     /locus_tag="PLZ74_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97776.1"
                     /translation="GFRRVWREYGRLAGVLRRTCLPASRCYGCGNLNFSVNFRFNLIH
                     PPYRAPDHLRNLGLREFWKLFDAYLNRTLLVSRRPPQPVSRVRSERVCPEIGKGWGWG
                     AVFAFV"
BASE COUNT         2347 a         4190 c         3885 g         2434 t
ORIGIN      
        1 gccagagggc gcgcgaaacg gcgagcgcct tgctggccgt gatgcggtca cgaccatcgt
       61 aaaggatctg cgaagggttg cgcggcaggt tgacgttgtt gcgcaacgag atgacggcac
      121 cctgcggcag ccccatggga tcggaggcgt agccgggcgg gatgccgttg gcgtcgttgc
      181 cgtcgcccca gatgcgagtc gtcggctggg ccgggttgtt gatatcaacc tcatagccat
      241 cctcccagtg gtcataatgg atgatggtat ccgggccggt gacgacgatc gaggttacgc
      301 tgtcgatggg cgccttgact tgcccaaggc cgtcgagagt gttgaaggcc ttcagcacct
      361 gcgcctcggg cagcggcagg tagaactcat gcaccatgta gacgccgcgg gcggataccg
      421 ccagaaacgc cgtcaacaac attaggctaa gaatttgttt atttcgaatc atcgcttccc
      481 tttccttgtc tatagcctga tctttttgtc acaccaaacc cgtgttacaa ccggtagggc
      541 ggtttccccg aaaccgcctc ggacgcctcg gggaggcgtc cctcccgata acctgaacgt
      601 tccggttacc ccttcgtttt cgcccggaac cccgggcggg acgcgtaacc tgttctgtaa
      661 atggattggg acggttggca acccctcgaa tctcgcaacc cgctgcgacc caaccggacg
      721 catcaataac ttgcgctcaa cactttcaac acggaacacc tctcgcgcca aattatcgca
      781 tatttcggca cgcctgtcta tcaccgcagg cggggcaaac gggcccgccg ttttatcccc
      841 tcttttgctt gtgtttccag cctatccgat gctatgctga ccgcatctta gccctctttg
      901 tttttcagga ggtgacccga tatgacgaag cgaactcttt acattagcga gatggatcgc
      961 cataaacttg aagaattggt gaatgtggcc catctttccc atgaccgcga tcagggggat
     1021 ctgaaatcgc tgcagaacga gttgcagcgc gcgctggttg tcgagcccga gcaggtgccg
     1081 ccgaatgtgg tgaccctcca ttcgcgggtc cgtctgcgcg aggtgaagac gcagaccgtg
     1141 atggaagtga ctttggtgat gccgtccgag gcgaatgtcg acgcgggaaa actctctgtg
     1201 ctgtcgccgg tcggtaccgc cattttgggt tacgccgagg gcgataccat cgaatggatg
     1261 gtgcctgcgg gccaccgccg tttcgtgatc gaaaagctgc tctatcaacc cgaagcgtca
     1321 agccgcagtt cacgcaaatg aggccgcggg catgaccatc gacggccgcc ccgtgacgct
     1381 gaaaccgggg atgacggtgt atgaggcggc acaggggatc ggtatccgca tcccggtgtt
     1441 gtgtcatgcc gacggagtcg tgcccgcggg cgggtgcggc ctctgcacag tggaggatac
     1501 cgctgcgggc ggaaagctgt tacccgcctg tgcgacgcgc gcggacgccg ccatgtccat
     1561 cgttaccgat tccgaagcgg tccgcaactt ccggcgcacc gcgctggaac tcctgctgag
     1621 caaccatccg gcggattgtg aggcgccctg ccggatggcc tgcccctcgg ggctacccgt
     1681 ccaaacgctg atggcccgga tccggtcggg tgattggcag agcgcgcggg atctggcccg
     1741 cgcccatccc tttgcgtgcg gcggagccgc accatgcgaa aaagcgtgcc gccgtgccct
     1801 gctggacggc gcggtcgcga tctgcgcact ccaccgctgg ctggccggcg aaaccgccgg
     1861 ggaggcgtgc agccgcgtgc cgggcggcac tcaggcgcgc ccccgcttcc gatcacggat
     1921 gcccgatttg aacgtggcag ccctagccgc gctgactccg gagaccggac cgcgccgcct
     1981 gccgccggag acgcacgaac ccacacccga agctgccgcg ttcgaggccg ggcgctgcat
     2041 gcggtgcggg tgcctgaagc cgacgaagtg ccggttgcgc gagttgtgcg ccgaatacgg
     2101 cgcgaacccg cgggcgttcg accacgcgcc gcgaccggtg atccgcgaga cgggccgcaa
     2161 cggattccgc ttcgatgcgt cgcgctgcat actgtgcgga ctctgcgtgc ggattacgcg
     2221 gcagtgtggc gcaggggagt tggggcccgc ttttcacgga cgaggcttcg aggcgcggat
     2281 cggcccacca ctcggccgcg tgtgggacga tcttccggac agcgtgctgc aggcctgtgc
     2341 cgccgcgtgt cccacgggtg ccatggcttt tgaaacaaac gccgtcgtgt gacgcgcctc
     2401 acgcccaccg gatcgccatg gcgccccacg tcaggcctgc gccgaaagcg tcgatcacca
     2461 gcacgtcgcc cttcttaagg cgcccctgtt caagggcctc gcaaagcgcg atcgggatcg
     2521 tggcggccga ggtgttgccg tactccgcga tattgcggaa caccttctcc ttgggcagcc
     2581 ccaaccgttt ggccaccgcg tcgatgatcc gcacattcgc ctgatgcggg atcagcagcg
     2641 acacctcctg actcgaaagt cctgcctgat caagcacaca gaccgcgcac tcgtacatat
     2701 tgcgcaccgc ctgcttgaaa acctcgttgc cctgcatccg gatgaagcgt ccgttctctt
     2761 cgggaaaaga ccggcacccg ctgttcggca aatggagcag tccgccgaac gaaccgtcac
     2821 cgcccagttt ggcggagagc acgccgtccg tgttcgagcg gtgctcgctg aagattgccg
     2881 cgcccgcgcc gtccccgaag aggatgcagg tgtcgcggtc ggaccaatcc acgatcgcgc
     2941 tgtttttctc cgcaccgatc accagcacat ggcgcagggc cccggactgg atcatacccc
     3001 aggccagact ggcgccatag atgaatcccg tgcaggccgc gctgatatcc agcaccccgg
     3061 cgttggacgc gccgagcgcc tgttgcacct ggcaggccac ggaggggtag atcatatcgg
     3121 gcgtcgaggt ccctacgagg atcaggtcga tctgttcagg tttcaggccg gagagggcca
     3181 acgcgtcacg cgccgcccgg atcgcgaagt cagaggtgtg ctccccctcg gccgcgatcc
     3241 gccgctcgcg gataccgaca cgctgggtga tccaggcgtc attggtgtcc acccgtttct
     3301 cgagatctgc attggtcagc cgcccttcgg gaagcgcctt gcccacgccg gttattttcg
     3361 ccgtcagttt tcgcgcactc atttcgtccc cgtgtttcag tccgacagta tagggtattc
     3421 tcaaacaggg aacaagtccg gcgcatggcg tgattcgcat tcccgagtca cggacataaa
     3481 tttcattctg gtttttcccg ccgacgtgaa gctgccggaa accccggctt gcctgacggt
     3541 ctcggcgggc ccggaatcat caggaccaga aattcctgcg caggcaccag atggcgccag
     3601 agaaaggcct gcatgcaatc ttcggctgcc acggcgtcgc ggatgatcgc cccttccccc
     3661 tcacctgaca cgccttggat cacgatcggc accggcaccc cggaatccga tggttgacgc
     3721 gcccggatca tgaggcgcgc cacggtttgc gtgcccgtca gggtcgcgcc ccgcacctca
     3781 aatcccgcgg caggctcccg gatgcggaac gtgatgggac cggcgaatcc gtctttgcgg
     3841 agggcgtgag ccgtgaccgg ggtctccccg ccgcgccgca caaccacccg cgacggcacc
     3901 acgcgcaggt caaaatccgg ttgcggctca ctgagccgca agcgatagcc gaaggcctcg
     3961 ccgccctgat gctgcgtatc ccggagctgc accgcatagg cgccgtccgc gggaagggtg
     4021 acgcgcacat acgcgtcggc atggtgcgtg tccagtccgg aacccggatc ttcctgatcg
     4081 tcattccacg ccaaagtccg cccctgatcg tccatgatct tcagccaggc gtcaaggggc
     4141 gaaccgagtc gccgcgccct gacctccgcc acgacctcct gtccggcccg accgcggaac
     4201 gtgaagacat ccaaatcgcc cggcataccg atcgtcccat tcatcacgat cggcagggtc
     4261 accggttgcg cccgtttaag cgtattgttg ggttcgtgct ccattccgtc gggcaacgtg
     4321 ctgatctcaa aaacaaaggg attggaacgc aatccgcccg atcccgcaac cgtgagcgtg
     4381 tgcagacccg gaagccgcgt ccgcaggtcg ggggtcagac gtgtctccgc gagatggaaa
     4441 cccttgacct cgatcaccgc atcgacaccg gcctgcccgc cgagcgggaa cacgctcgtc
     4501 acaaacggca actcgccgat ggtcatccgg tagacgaaat cctcgcgtcc gcggaaaatc
     4561 gcgtcatgga tcgccaacac gtactcgccg ttcgcggaaa gctcgcagta caggacaggg
     4621 tccggatgga agcggtaatc gtcgttgtat gccttctcgc gtccctgggc gtcgcacagc
     4681 accagcacag gctgaaacca gccgggaacc gcatccgcca gataggggac gagcgcgcgc
     4741 gcctgtacgg tcacgaccag ccgttggccg ctccgcccgt caaaccggta gcggtcgacc
     4801 gatcccgagg cgatctggcc gttgaccgtg cagggcggca tgatccgcac ctcgcggtca
     4861 tccacatccg tcagcgcgcc ccggcccgcc atgcccgaga ccatctggat catttccgct
     4921 tcccgctcca ccatcccgcg ccgtccccga ccctttccgg ctgcccccct tttcctgccg
     4981 gaagtgctgc gcaggctctg ggcctccttc ccgagaatct gcttcgggct ggtgggaagc
     5041 ggctcggacg tgacctcggg caacgcgccc acatgaaacg cgagcgggtt ggacactccg
     5101 cgcggtgtca ccagccggat ttcgcggggg ccgggcggcg tgccgcgctc gaccgcgacc
     5161 tcgaccgtca cgagattggc gatggaggcg cactgcggct gctgcacgta atcgccgatc
     5221 agcatctcca ggcgcgacgc cagattggtg acctgcgcgt cgcgccgcgc tttgggcatc
     5281 gcccgaatct cgtccaattg ttcccgcagc agttgcacct cctgcgagtt catgtgcctg
     5341 ttgtactcga tgacgcgccc cgtcacccct gatcccgtaa agatgacgcc gttgaccccg
     5401 tcgagcgcct ggccccctag tgtgcagcgg aaaaccgtgc cctgccgtcc tcccgccggg
     5461 aaaacgaaac cgatgtaggc gggttgctgg gcgcacagga atcccggcag gacggccagc
     5521 agcgcacgga atgcccatga ccgcagcatc ggccgcaggc ggctttccct tcccgcaacg
     5581 tgagcgttgg ccgttcccgt tatcccatcg cttgttcccg ttccgtgggc ggcgggttgg
     5641 ttccatgccg tcatacccgg ccccctttca catgatctct ttcaggcgtc ccgcgctctt
     5701 cgcgcccgcc tcgcgcatat ccagcacacg cgcgtccgcc ccttcgggat gcggcaacgc
     5761 ggcctccgtg tcgatcccgg cgagttcata aatggaaccc agcagatcga tcggatagat
     5821 ggggcgctct tccacctctt cgcctttggc ggtcgaggca ccaaccagat gcccgccccg
     5881 gaaacccccg cccgcgacca atacggaaaa aacgttgcca tggtgattcc gcccgccgtt
     5941 ccaaggcggc tgccagtcca ccttgggcgt gcggccgaat tcgccgcagc accagacgag
     6001 cgtgctttcc agcaacccgc gttccgccag atcctgcaac agcgcggcga gtccctgatc
     6061 cagctgcggg cattgccggt tcatggtttg aaaatggttg gagtgggtgt cccagccacc
     6121 cggatagttg atcgtgatgt acggcacgcc cgcctccacc agacggcgtg ccgcgagaca
     6181 atcctggccg aacgtgtggc ggccgtaccg gtcgcggatc gttgcctcct ctttggacaa
     6241 gtcgaaaacg tcgcggccct gaccgaggat caggtcgtat gcgcgtttct ccgcctcgcg
     6301 cgccgccgcc aattcggggt ggcccgacag ggtgttgccc agcgtgttga gcgaggagag
     6361 cagcccgcgc cgcgcttcct gccgctgatc ggtgatgccg cgcgcgatca cgccttcgac
     6421 ttcgaagcgc ggagcattgg ggtcgccgcc ggtcgcgaac ggcttgcagc gcgaaccgag
     6481 gaatccggct tcggaaaagc ggccttgggg ctgcgtcagc accacatagg gggggatcag
     6541 tcctgtgtag cctgccgcct cgcctttgaa cagcgcgaag accgcgccga ttgcggggta
     6601 agccaaccgt tgccccggca tatgcccggt ctgcatcaga tacgcggcgg tctcgtgccc
     6661 gttgtttcgg tgcgtcatgc tgcggaggat cgaaaacttg tcggcctgtt gcgccagctt
     6721 cggaaaaagc gcgccgagac ggatgccgtc cacgttggtg ggaatgacat ccttgaacgc
     6781 gccggtgtaa tcgtaacccg cgtccggttt ggggtcccaa gtgtcggtgt gcgacatgcc
     6841 gccccagaga aaaacctgga tcaccgattt cgcggtcgcc ttgcgcgccg cgggcgcggc
     6901 ctgcccgcga accgtccgtc cgggccacac cgcgacggcc gcaccgcata agccggtccg
     6961 cagcagatcg cgacgcgtga cggatttccg ttctcgcata cacacctcct tctcagtgcc
     7021 ggtacaggaa ctcgtcgctg ttgatcaatg cccagataag gtcgacaccc gccgcgtctt
     7081 ctttcaccac cccggagcgc aggtagcgtt cgacggttcc gatctccgtg gcggacggga
     7141 accgcgaaag ggttgtgaga taggcttctt cgatcatggc gcgcgaaccc cgtttgccgc
     7201 ggaacatccg gcgtaacacc gggctggtct cgagtttccg ctggatatgg ctcgaattga
     7261 gcagatgcat ccgttgcgga ggcagcgcgc ggttgatccg ctcgctcgca aggccggtgg
     7321 cgcgtgccgg acgaccgaat aactccagaa acgggctggt gatcgacccg tccggcagcg
     7381 cgaccgcagg tttgttggcg ggaataaagg tgaagggttc gggaatcgcg ctggtgtaga
     7441 ggtcggacgt gccggtgact ttgttgatca cgtcgatcag cacctccgcg tcgagttggc
     7501 gcagcggata atacgcgaac agcggcgcgg cggactcgac ccgcccggac gggaacacgg
     7561 gcgagaattg gtaggtgcgg gaggtgagga tcaggcggta gagcttgcgc aggtcgtagc
     7621 cgctccgcac gaactcacgt tgcagccgct tcagcaagtc gggatggctt ggcggattgt
     7681 cggaccggat gtcgtccggc tcgtggatga tcccacggcc gagcagccag taccagacgc
     7741 ggttgaccag cgcgcgcgtg aaccacgggt tctgcggcgt gatcagccat tcggcgaacg
     7801 ccgtccgcgg gtcgcgtcca ggctccagtc ggaccgaggt gccgtccggc aggcgaccgg
     7861 ccgtcgtggt caaaggctgt tcgggatccc agaagacgat ttcctctttc cactcccggg
     7921 tgggtttgta ccgaacctgc gagaagaaca ccgcgagatc ggccagcgtg ttggagggcc
     7981 agtgctcggt ccgtgcgccc atcagcgtga gcgcgacggc ggaggcgagc ccttccggcg
     8041 aacggtccgg cgtggcgcgg tagaaattca ccggcccgac gcggaaattg ctgccgttgg
     8101 acagcagcag ctcgcgcgcg aaacagtcgt acggcttgtt gtcgcgcatc gcggtgcgga
     8161 tccagcgatg ataggcctgg gcggcattcg gccagaggtt gacgggaaat tccgctttga
     8221 cgcgcagcag atcactccag cgcatggccc aatagtcggc gaactcatca cgctcaagca
     8281 ggcggtcgat caacgccgtg cgcttgtgca tcgaggtgtc ggcgagaaag gcacgggcct
     8341 cgtccgcggt cggcagtgtg ccgagaatgt cgagataggc gcggcgtaaa aaaaccgcat
     8401 cggtgcaata gacgggcgta atcccgtggc gcttcagttt ccggaagatc ggcgcgtcga
     8461 ccgggttctc gggtgtgggt gtgccggtct ggaggaaaag cgcggcctcc ggcgctggcg
     8521 cgccgaaccc tgcccgcacc gcaacgatca gacatacaat ccgccacatg cgaagccccc
     8581 tatccgcaca ctccaccaca gatatccgaa tcgtatcctt aaacggcggg acagccaatc
     8641 tatttcccgg tgccggtacg caaccgcatg cccgccctac cgcaccttga gcaggaagaa
     8701 gctctttttc ccgctgcgca ggaccgtgag gcgaccttca cacaggtcag aagcgtcgaa
     8761 cacgcgttcg attcccgtga cgcgcgcgtt gttgatatac agaccgccct gctggaccag
     8821 acggcgcgcc tcgcctttgc tggcaaccat gccggccgcg gcaatcacct caaacgcggg
     8881 tttgccgagc acgtgatccg ccggcaacgt ggcggagggc acattaacga aaatcgcctc
     8941 cagctccgcc gcagccagcc cctcgatcgc gccaccgaac agcactcccg tggctttttg
     9001 ggcgaccgca agcccgttct caccgtgcac ggtgcgcgtc acttcctcgg ccagcacctg
     9061 ctgcgcctcg cgtttctccg gcgcggcggc aaccgcctgc tccagctccg cgatacgccc
     9121 catgggcaga aaggtgaaga tcttgaggta tcggatcaca tccgcgtcca gtgtgcgcag
     9181 gaagaactgg tagaactcgt aataggaggt cttgcgcgcg tcaaggaaga tcgcgttccc
     9241 ctcgcttttg ccgaatttct tgccggtgct gtcgcacacc agcggaaacg tcatgccgaa
     9301 aacctcttcg ccgcagatcc gccgcaccag ctcggtgccg gccgtgatat tgccccactg
     9361 gtccgaaccg ccgatctgca gcgtgcaccc gaaagtccgg tgcagatgat agaagtcata
     9421 cccctgcatg atctggtaac agaactccgt gaaactcatg ccgtcatcgg actccatgcg
     9481 ctttttcacg ctgtccttgc tgagcatcac gcccatgcgg aaatagcggc ccacatcgcg
     9541 caacagatca atgaaggtga aagacttgaa ccagtcgtaa ttgttcacca gaatcgccgg
     9601 gttggcggag tccgggttga aatcgaggaa gcgcgacaag ttttcccgaa tgccgacgag
     9661 gttgtgctcg acctgctccg cgctcaacag attgcgttcc ttggatttgc ccgacgggtc
     9721 gccgatcaag cccgtggcgc cgcccaccaa agcgattact ttatgcccgg cgcgctgaaa
     9781 atgcgccaag gccatgatcg tcacgaaatt gccggcctgc aagctgtcgc tcgtggggtc
     9841 gaaccccgcg tacacggtca ggggcttgtc cagcgccttc gccagcccgg ggctggtcat
     9901 gtcttcgaac agatggcggg ctttaaaggt gtcgaggatg ttcatggggg agaggggggg
     9961 atagcttgtt gtcgagagaa ccgatggatt cgaaagaatc gatttaatcg agtgtgtcga
    10021 ttctctcgat tccatcgaaa aaaaggacgg ggcgcctgtc gccaggcgcc cttttttgct
    10081 tactccgcgc ccggctgcca ctcttcgctt tgggcgaagg cgtcatcaaa ggccgccgca
    10141 aggcccacca gattctcgcc cggcttgagg tccacggtgc tcagatcacg cgtcagctcg
    10201 gcgatctcac tctcgtccat gctcacggcc ttgatgctca ggccgatgcg gcgctcgtcg
    10261 cggtcgatct tcacgatgcg ggcctccaca tcctggccga ccttgagggc gtccttaacc
    10321 ttctcgacgt gctgatcgct gatctgggag atgtggacca gtccgtccac gccatcttcc
    10381 agctccacaa aggcgccgaa ggaggcaatc ttggaaacct tgcccttgac gatctgtccg
    10441 atcgcgtacc ggctggtgat ggtgttccac ggatcgtcgc tggcctgctt gaggcccagg
    10501 ctgatgcgct gatcggccgg attgacctcg agcaccaccg cctcgacctg ctgacccttc
    10561 tgcagaacct cgctggggtg gttgatcttg cgggtccacg acatgtcgga gacgtggatc
    10621 atgccgtcga tgccctcttc cagctccacg aacgcgccgt acgtggtgaa gttgcgcacc
    10681 ttgccggtca cgcggctgcc gaccggataa cggtcctgca ccgtgtccca cggattcgcc
    10741 tcggtctggc ggatccccaa cgcgatcttc tggttggccg cgtcgatgct cagcaccacc
    10801 acgtccacct catccccgac gttcagcacg tccgaggcgc gcgccacgcg cttggtccaa
    10861 gagaactccg agacatgcac cagcccctcg atacccggct ggatctcgac gaaggcgccg
    10921 tacggcacca gattcacgac cttgccgcgc aaacggctgc cgaccgggaa ggtcccttcg
    10981 atattctccc acggattggg cgtggcctgc ttcagcccca gcgaaacccg ttcgcgctcc
    11041 atatccacat cgaggatgag cacatccagt tcctgcccca ccttgagcat ctcgctcggg
    11101 tgcttgatgc gcccccagct catgtccgtg atgtggagca gcccgtccag accgttcaga
    11161 tccacgaacg cgccgaaatc ggtgatgttc ttgacgtgac cgtgacgcat ctgccccttc
    11221 tcgatcgttc cgagcaattc gcgtttcttg tcggccatgc gctcttcgat cagctcacgg
    11281 cgcgacacga tgatgttgcg gcgctcattg ctgattttga taaccttgaa ctcgaacgac
    11341 tgattcagat agggatccgg atcatgcacc ggggtgacat ccacctgcga tccgggcagg
    11401 aaggcgtcca cgccgtccac atccacgatc agaccgccgc gcaccttggt gcggatggtg
    11461 ccggtgacca cgccgccttc gctgtatttg gcgagcaccg catcccagcg caacttctca
    11521 tcggcgcggg ccttgctcag gctcaccatg ccgctttcgc tttccagctc catgagcaac
    11581 acatcgatct catcgccgat cttcagctgt gtcacatctt cgaactcatt tgccggaacg
    11641 atgccttccg atttgtaacc gatatcaacg agaacctcgt tgcctttgat gccgctgatt
    11701 gtgcccttga tgatcgaccc ggccacaaac tgattcatct gctggtccag cgctgccgcc
    11761 atcatgtcgt acatgggacc ggttttggga gtttcggccg tgggtttacg aattgccatg
    11821 tgtaaggaat tccagtctgc cgccgcggca cgcgtggttc cgcgcacata tgggattcgg
    11881 gaacgagtcc cggcgtcaga cgttgggtta ggtgttgcac ttccggggcc gtcgcttctc
    11941 cggccacccg gccggctgca cgtgcccctt ttttgtcctg ttggggacaa aagatgttct
    12001 ttatagcaaa aaaccccttc gcgggacaaa ccaaaaaacg ggtatccgga aatccgaacg
    12061 ttcagcgttc aacccaaaag ctcgctcgta tcgaatctca cccgcgagat caccctcacg
    12121 ggcagaaatc atccaactga aaacccgttg ccgccaactc gactattgca cgttgcccat
    12181 gaatgtccta cactccccca cgcatatgct ttcggggcgt tgcacagcct ggtagtgcgc
    12241 cagctttggg agctggatgt cgggggttca aatccctccg ccccgaccat ttgcaaccct
    12301 gtttttgttg gaaaagccca ataaaagcgg ggtttttcat ttataccgcg tgaacgcccc
    12361 tttttcgtga aacaccctga aaaagcctga aaaagagggg gcatttacac acacaaacac
    12421 acacacaaaa caggggtgaa aacgcgccga tacgcttgcc cgcttggggt tttcagtgtt
    12481 ttgggggttt cgcgccgccc cttgcgcccg cccgtgtgtg ttttggaatt acacaaacgc
    12541 gaaaacagcc ccccagcccc agcccttgcc gatctcggga cacacgcgct cggagcgaac
    12601 gcggctaacg ggttgcggcg gtctccggct gacaagcaac gtgcgattga ggtatgcgtc
    12661 aaagagtttc cagaactcac gcaggccaag attgcggaga tggtcgggtg ctctataggg
    12721 tgggtgaata aggttaaatc tgaaattcac actgaaattg agattcccgc aaccataaca
    12781 acggctcgcg ggcagacacg tccgacgcag tacgcccgca agccgtccat attcgcgcca
    12841 gacgcgccgg aagccg
//
LOCUS       DAOSRB010000070        12678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4981_length_12678_cov_6.825477, whole genome shotgun sequence.
ACCESSION   DAOSRB010000070 DAOSRB010000000
VERSION     DAOSRB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12678
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4981_length_12678_cov_6.825477"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLZ74_05330"
     CDS             complement(<1..280)
                     /locus_tag="PLZ74_05330"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOR97777.1"
                     /translation="MSEKMVALAMAAHPDDIEFMMSGTLLLLKQAGAEIHICNLANGC
                     YGSQVYGKEEAARVRALEAQEAARVAGAVWHPSLFDDVGLFYNADSLAK"
     gene            complement(320..1378)
                     /locus_tag="PLZ74_05335"
     CDS             complement(320..1378)
                     /locus_tag="PLZ74_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR97778.1"
                     /translation="MKTFGAVRALDGVSVSIRPGELFFLLGPSGCGKTTLLRCLAGFH
                     TPDEGRIFFGTQDVTDLPPHARNTGMMFQSYALWPHMTLAENVAFGLEMRKRPRAEIR
                     ARVAAALELVHLADRAKARPNLLSGGQQQRVALARALVVEPQCLLLDEPLSNLDAKLR
                     LEMREEIRRICKAAGTTAVYVTHDQKEALSIADRIAVMRDGRIEQIGTPEEIYRHPAT
                     RFVAGFIGEGHFLTGRIESVSETEGVQVATRIGLFQARRAPGGIRTGEEVTVCLRPET
                     LRFGVPPHAGMNRMRGRYRASVYLGEMARHEIDLGPEDGPCEVLTAFQLNPSGGTDRT
                     GDPAEVWVAPDEVIVTRG"
     gene            1611..1895
                     /locus_tag="PLZ74_05340"
     CDS             1611..1895
                     /locus_tag="PLZ74_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR97779.1"
                     /translation="MILKVALRAIFRNKTRSLLTALGIIVGIAALIAVVASGQGAQAM
                     MVREISSIGNNIIMVFPGSRGALHGWRSDRCIGWHLRFLSRPPRLPSQSH"
     gene            1919..2608
                     /locus_tag="PLZ74_05345"
     CDS             1919..2608
                     /locus_tag="PLZ74_05345"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOR97780.1"
                     /translation="MTMTLSSSLLDFGDVRAALGRGGRVALFIRHSERPPIRSDDKDF
                     GRHLGLTPRGVAVAREAGAMLAGAPDVRLLASPMQRCRLTARHIAEGMGVPEPQVEDA
                     DPLGVRGFFYRDPYAVQEIMRQQGYMAYMLEYLKKGNALHSAPLMTAMEQTLEWMITQ
                     MTVPFVLFVSHDIFVAAILTALRLRSYTAEEWVGFLHGFALIQHGQDPWACHPCLPSH
                     TEVDASHPFIH"
     gene            2623..3249
                     /gene="thiE"
                     /locus_tag="PLZ74_05350"
     CDS             2623..3249
                     /gene="thiE"
                     /locus_tag="PLZ74_05350"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOR97781.1"
                     /translation="MLIERFGLYLVITDPVTSYERCAEAAVAAELRYIQLRRKKCDRA
                     AILATAHNLRTITRGTSTRLIVNDDPELAAEAEADGVHLGQDDMPLPEARRRYPVLRH
                     FGLSTHTPEQARSAIKVAPDYCGVGPVFATPTKEVPDATLGPEQAGAIIRAAPFTTVA
                     IGGIDADRLPEVLRAGAVNFAVVRYVCQAPAPFDAIRRLQDIWQAAQP"
     gene            3287..5731
                     /locus_tag="PLZ74_05355"
     CDS             3287..5731
                     /locus_tag="PLZ74_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOR97782.1"
                     /translation="MMRCLLIPLLFYILCRTTASALPEINLITEPACNSEVAVIVGHG
                     FVPGRTRVKALALGGYDMRKPQDPLRFAKAIGRPPDLPDTPPPEAQECEIVGQGEVFL
                     QVLFRCLPQSWVCAPLTSAVWVGDESGWGRPILINRPQAQWLYPQRQAPGEPIRIFGR
                     TFAWGWLLPSAHVLLRPVGSTQMVHLARAAQHHEDGHTERWSLCVWLPESLAEGEYEV
                     FVHGRHGAEYGWSSPLRLTVAQKPSSRPVVSVREHGAVGDGLADDTAALEAALRQAAP
                     GGTLFLPAGTYAISRTLNIPEDVVIQGAGMRQSVIANRQEPCVDPFALTQTRPVFDGK
                     DLLWGMRRFALRDLTLRFMPALGSALRVGRDPLWSEDVSLYRVRLETQQDFGLSPTHP
                     YCARPLNIVKARRFSMVRCETYGPGACSCERKVEDSLFSQNRFITDRRWRGHGFKFWG
                     AEQVIFEDNLLSGDTRGLVMQTHFGVNYRNFIAGNTVERTVLGENAGETYLVEGAGYL
                     GESAVEEATPTHVRIARWPTARGAPATAEMCVGRFVVIAGGRGIGQWRRVRAADPAQR
                     VLYPETPWRVPPDASSVVVMMNGLIDTVFVNNQETDCGKGLYIYGAGAINCIIDRHLC
                     ARTLGITFKTHDDRQNADPMHHQTAPDFFNLIRDCRVHDGGGLFCAVGGRLPLEERNG
                     VPWANFANRFIANEIQNVTPFSGAQYGANWTWGGGWDNVQAGISVIPSDLGTAAGSGS
                     PGPERIRAATFQKNWISGSRFGVGVSRRAADTLLMQTTVYDVDVPLTDKGRDTREKAT
                     VVRTPETYSPERGPIR"
     gene            5816..7396
                     /locus_tag="PLZ74_05360"
     CDS             5816..7396
                     /locus_tag="PLZ74_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97783.1"
                     /translation="MRNQWQTKQLARRCVIALPFLLFLVSSGCKPSAKRAAEREAYLK
                     HEVEQTSLRIQDYSYERQDEALVLVDEALANPRFAGHKPIFFAHKINLLLSLERVKEA
                     SDLVVEAWKKAPDQAQAVFGKICDFHQQRNAYREMNDWCTRLLDLGAAFPKELRGRLM
                     LRRLTAALGIKDMEQARSDLDTLLTLVRPEESLPDLQRSLEWFIDMGNHREALVLLNH
                     FEKAAAGQPSYQPLIVSLKLRCIVLTGEWGRVVEALAACVPQLPDAMLHQTLKNVFGA
                     LNKNNQTDLLQQAAKQILFNAVGKEASVHYAARVWVDTGMTVDKKVLAERLNVLLNVK
                     VSPILVGDLYERYFYETIEDHNVVRNLCAVGERLLTVCTNEATVNMAKVKVLDGAFIV
                     NDYDKAIHMLEQGIPGKDKAWHDMSLPKVKAHRAMAQNKPREAVAYFRAFMNAWLATD
                     QDEESDPTTGISHSREWILGRNAIRIARLLESIPDKEEADKAREEAKDYFRKALIKAE
                     NDPPALKLLKEEIRPLGL"
     gene            complement(7428..8510)
                     /gene="ispG"
                     /locus_tag="PLZ74_05365"
                     /gene_synonym="gcpE"
     CDS             complement(7428..8510)
                     /gene="ispG"
                     /locus_tag="PLZ74_05365"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOR97784.1"
                     /translation="MRCPRTATRQVHVGSVAIGGGAPVSVQTMANADPHDAEALLRQV
                     CEAAELGCDLVRLTVPDHAAAETFKQVRARSPLPLVADIHFDHRLALAALAAGADGLR
                     INPGNIGGPERVRAVVAAARPNRIPIRIGVNGGSLEKDLLARYGSATPEALVESALRH
                     VAWLEAEDYREMKISVKASDVPRTLAAYRLLSERTDYPLHLGVTEAGTFLPGTVRSCV
                     ALGTLLLEGIGDTIRVSLTDQPAQEVRVGVELLRSVGLRAPSASVTSCPTCGRTRVDV
                     AGLALRIENRLERFYREHPDAPRPHVAVMGCVVNGPGEAKGADIAVAGGDGVFVLFVR
                     GERIRTLPEAEALEAIVRLVTEWPRP"
     gene            complement(8536..9981)
                     /gene="rseP"
                     /locus_tag="PLZ74_05370"
     CDS             complement(8536..9981)
                     /gene="rseP"
                     /locus_tag="PLZ74_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOR97785.1"
                     /translation="MEWVSLIGSGVCVALFFSLAIFIHEFGHFIAARRLGLRVDAFSI
                     GFGPALWKRTVGGVVYKVGCVPFGGYVALPQLDPSGMEKVQGSERDGEGDSCSELEDI
                     APWKRIVVALAGPVGNFVLAIFLAYFLFLAPGVKTGVLDTRIGSVAENSEAWKAGVRP
                     GDRILSVNNRKVGTWSDLQVECQLAGDSGKAVFGIQRGDTVREVELPFRTSEIVELKM
                     LEGVFPATHCEVSALMPDMPAIQAGLLPNDVVVEINGEPVREAQQFAEAIQHNRAQPV
                     TLTVKRGRELQQIVLTPRFDEASGRYLVGIVWKDATERVRAWMMYRNPWRQLKWDSLS
                     VVRVLQALLAPESKGERKAVARNVGGPVAIVIGLFHTVRGDILDAIGFLRMICVNLAI
                     LNLLPFPVLDGGHILFALYEVTTRRKPHPKVVSALVNAFAFLLIGLMVLLFYRDIARQ
                     VKISRAVRAAVREARQEAETASETNASVVPR"
     gene            complement(10047..11255)
                     /locus_tag="PLZ74_05375"
     CDS             complement(10047..11255)
                     /locus_tag="PLZ74_05375"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOR97786.1"
                     /translation="MRSVVILGSTGSIGENALRVIEAHPEALRVAGLSTRTHIMRVLD
                     QALRFDVPVVAVADENAAREAETLARPHGIEVWPGEAGVAALAALPKADMVLCALVGL
                     AGLEPVLEAIRVGHDIALATKEVLVAAGELVMRRRAAAGVRMLPVDSEHSALFQCLQT
                     PGCPPACVRDAADTSRIEARVARFALTASGGPFGASKETDFDRVTPEEALTHPRWDMG
                     RKVTIDSATMMNKGLEILEAHWLFNLPVAKIDVLIHPESVVHSMVTFTDGATLAQLSM
                     PDMRFPIQYALTWPERLTADLPRLDLARMGCLTFAEPDTTRFPCLRLAREAAQAGGTA
                     PAVLNAADEVAVEAFLAGAIPFSGIARAVEAVMAQHDVKTCVELETILEADAWARRQA
                     GAQVKKSGTT"
     gene            complement(11252..12160)
                     /locus_tag="PLZ74_05380"
     CDS             complement(11252..12160)
                     /locus_tag="PLZ74_05380"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOR97787.1"
                     /translation="MLMRRILAGVLVGVAWLLSLCAMPGWMLFGVLLVMSSACQNEFY
                     QMMKRNGYPVSRNGGMVMGVVWLTACYAFPPYAAGRLPTGHQFETLMLAMLVAALLLR
                     VLFDSRIAKPVEHVGVTLLGFFYLPFMLSFFIRLAQWGADEMFEIPSARAGVFLASYL
                     AAVVKFSDVGAFAVGVTMGRHKLFPRVSPKKTWEGLVGGIVVSMMVSAGLVAAAKRFD
                     CLPGGPLAELSMTHALVLGLVLSVVGALGDLIESMFKRSVNAKDSAGLLPGGGGGILD
                     MFDSLTFTPAVLYFYLVWFVGQSVAL"
     gene            complement(12154..>12678)
                     /gene="uppS"
                     /locus_tag="PLZ74_05385"
     CDS             complement(12154..>12678)
                     /gene="uppS"
                     /locus_tag="PLZ74_05385"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOR97788.1"
                     /translation="LMALLGTFIHKHERELIARQVRLRVIGQREDLSAPLREALTRLE
                     RETAHFERQLLIALSYSGRNELARAARRIAEEVRAGTLTPEQVDEAAVAARLDAPDVP
                     DPDLIIRTSGEWRISNFLLWQSAYSEFFVTPVLWPDFREAEFQAALAAYAKRERRFGG
                     VTAQPAGAGGGAPC"
BASE COUNT         2485 a         4220 c         3647 g         2326 t
ORIGIN      
        1 ccttggccag cgaatcggca ttatagaaaa gtcccacatc gtcgaagagt gacggatgcc
       61 agaccgcgcc ggccacgcgt gccgcttcct gcgcctccaa tgcccgcacg cgcgccgctt
      121 cctctttgcc gtaaacctgt gaaccgtagc agccgttggc cagattgcag atatggatct
      181 ccgcgccggc ctgtttcagc aaaagcagcg ttccgctcat catgaactcg atatcatccg
      241 gatgtgccgc catcgccaac gcgaccattt tctcactcat tttcgttctg acctttcccc
      301 tctttctgcc cgctgcgttt cagccgcgtg tcacaatcac ctcgtccggc gccacccaga
      361 cctctgccgg gtcacctgtc cggtccgttc cgccggaagg gttcaactga aacgccgtca
      421 gcacctcaca cggcccgtcc tccggaccca aatcaatctc atgccgggcc atctcgccta
      481 gataaacact cgcacgatag cggccgcgca tccggttcat tccggcgtgc ggcggcacac
      541 cgaaccgcag cgtttcggga cgcaaacaga ccgtcacctc ctcgcctgtc cgaatccctc
      601 ccggtgcgcg gcgggcttgg aacagcccga tccgcgtcgc gacctgcacc ccctcggttt
      661 ccgaaacgga ctcaatgcgg cccgtgagga aatggccttc accgatgaac cccgccacga
      721 accgcgtggc gggatgacgg taaatctcct cgggcgttcc gatctgctcg atgcgaccgt
      781 cccgcatcac cgcaatccgg tcggcgatgg aaagcgcctc tttctggtca tgcgtcacat
      841 acacggcggt ggtcccagcc gctttgcaga tgcgccggat ctcttcgcgc atctccaggc
      901 gcaatttcgc gtcgagattc gagagcggct cgtccagcaa caggcactgc ggctccacca
      961 ccagcgcgcg tgccagcgcg acgcgctgct gctgaccgcc ggaaagcagg ttgggacgcg
     1021 ctttggcgcg gtctgccaga tgcacgagtt cgagcgccgc tgccacgcgc gcgcggattt
     1081 ccgcgcgggg ccgcttccgc atctccagac cgaaggcgac gttctccgcc aaggtcatgt
     1141 gcggccacag cgcgtaactc tgaaacatca ttccggtatt gcgcgcatgc ggcggcaggt
     1201 cggtcacatc ctgcgtcccg aaaaaaatcc gcccctcatc cggagtgtga aatccggcga
     1261 gacagcggag cagcgtagtc ttgccgcatc cgctggggcc caacagaaag aacagctcgc
     1321 ccggacggat cgacacgctg accccgtcca gtgcgcgaac tgccccgaac gttttcatga
     1381 tcccttgcgt ttcgaccccg actgccatgt cccgctccga cccttcccgt agccccatag
     1441 ccaacctagc ttttgtggtt taagtatatc gattgtcacg gcacagggca agcatccagc
     1501 agttttctgc ggaaatcgat cccgttccgt tcttccatga ggctttcaag tggtgtatga
     1561 taattgcttt aagacacaaa ccggactcac gccatcccat gacctttttc atgatcctga
     1621 aagtggcgct gcgggccatc ttccgcaaca aaacgcgttc gctgctgacc gcgctgggca
     1681 tcattgtcgg cattgcggcg ctcattgctg tcgtggccag cggccaaggc gcgcaggcca
     1741 tgatggtccg cgaaatcagc agtatcggga acaacatcat catggtcttt cccggaagcc
     1801 gtggtgctct ccacggttgg cggtctgatc ggtgtattgg ctggcacctt cggtttttat
     1861 cccgccctcc gcgcctcccg tctcaatccc attgattgcc tccgttatga ataaacgtat
     1921 gacgatgacg ttgtcttcct ccctcctcga cttcggtgac gttcgggccg cgctggggcg
     1981 cggtggccgt gtcgccctgt tcatccgcca ttccgaacgc ccgcccatcc gttcggacga
     2041 taaggatttc ggccgacatc tcgggctgac cccccgtggg gtcgccgtgg cgcgtgaggc
     2101 gggcgcgatg ttggcgggtg ccccggacgt gcggcttttg gcaagtccga tgcaacgctg
     2161 tcgtctgacc gcacggcata tcgccgaggg catgggggtt cccgaaccgc aggtggaaga
     2221 tgcggacccg ctgggtgtgc gcggtttttt ttaccgtgac ccctacgcgg tgcaggagat
     2281 catgcgtcag cagggataca tggcttacat gttggagtat ctgaagaagg ggaacgccct
     2341 ccactcggca ccgctcatga ccgccatgga acagacgctg gagtggatga ttacgcaaat
     2401 gaccgtaccc ttcgttcttt ttgtcagtca cgatatcttc gtggccgcta ttctcaccgc
     2461 cctgcggctc cgctcttaca cggcggagga gtgggtcggg tttctgcacg ggttcgcatt
     2521 gatccagcac ggtcaagatc cttgggcgtg ccacccatgc cttccgtcgc acaccgaggt
     2581 cgatgcgtcc cacccgttta tccattgagt tccggagact gtatgctgat cgaacgtttt
     2641 ggcctgtatc ttgtgatcac cgaccccgtg acctcatacg agcgctgtgc tgaagccgct
     2701 gtggcggccg aactgcgcta catccaatta cgccggaaaa agtgtgaccg ggccgcaatt
     2761 ttggccacgg cgcacaatct gcgcaccatc acccgcggca cctccacgcg cctgattgta
     2821 aacgacgatc ccgaactggc ggcagaggcc gaggcggatg gtgtgcacct cggccaagat
     2881 gacatgcccc tgcccgaggc gcgccgccgt tatcccgtcc tgcgtcactt tggcctctcc
     2941 acacataccc cggaacaggc gcgctccgcc atcaaggtcg cccccgatta ctgcggcgtg
     3001 ggaccggttt tcgcgacgcc gaccaaagag gtccccgacg ccacattggg gccggaacag
     3061 gctggcgcga tcatccgtgc ggcgcctttc acaaccgttg cgatcggcgg catcgatgcc
     3121 gacaggttac cggaagtgct gcgggctggc gcggtgaatt tcgccgtggt gcggtatgtg
     3181 tgccaggccc cggccccctt tgacgccatc cgccgtctgc aagacatttg gcaggcggcc
     3241 caaccttgat ctctccatgt cccgtgaata cgaaaggagt ttcctgatga tgcgttgcct
     3301 cctgatcccg ctcttgtttt acatcctgtg tcggacaacc gcatccgccc ttccggagat
     3361 caacctcatc accgagccgg cgtgcaacag tgaagtggcc gtgatcgtcg gacacgggtt
     3421 cgttcccggc cggacgcgcg tcaaggcttt ggcgctcggc ggatacgata tgcggaagcc
     3481 gcaggatccg cttaggttcg cgaaggcgat cggccgtccg cccgatcttc ctgacacacc
     3541 accgccggag gcgcaggagt gtgagatcgt gggacaggga gaggtcttcc tgcaagtgct
     3601 gttccgctgt ctgccgcaat cgtgggtgtg cgctccactc acctccgccg tctgggtcgg
     3661 ggacgaatca gggtggggcc gtccgattct catcaaccgt ccacaggcgc aatggttgta
     3721 tccgcagcgc caggcgccag gagagccgat ccgaattttc ggcagaacct tcgcctgggg
     3781 ttggctcctg ccctctgccc atgtcttgtt acggccggtg ggttccacgc aaatggtgca
     3841 tctcgcccgc gctgcccagc atcatgagga cggacacacg gaacgttggt cactctgcgt
     3901 ctggctaccg gagtcgctgg ccgaaggcga atacgaggtg tttgtccatg gtcgtcacgg
     3961 tgcggagtac ggatggtcca gcccgttgcg tctgaccgtc gcccaaaaac cgtcgtcacg
     4021 gccggtcgtt tcggtgcgcg agcacggggc ggtcggcgat gggctcgcgg atgacacggc
     4081 ggccttggag gctgcgctcc gtcaagccgc acccggcggc acgctttttt tgccggccgg
     4141 aacgtacgca atctcccgaa ccctcaacat cccggaagac gtggtgatcc agggcgcggg
     4201 catgcgccag agtgtgatcg ccaatcgaca agaaccgtgc gtggatccat ttgcgttgac
     4261 acaaacccga cccgtttttg atggaaagga tctgttatgg gggatgcgcc ggtttgcttt
     4321 gcgtgatctg accttacggt tcatgcccgc gctcggttcg gcactgcgtg tcggccgtga
     4381 tccgctgtgg tccgaagacg tctcgctcta ccgggtccgg cttgaaaccc aacaggattt
     4441 cggactgtcc cccacgcacc cctactgtgc ccggccgctc aacatcgtca aagcgcgccg
     4501 cttcagcatg gtgcgttgtg agacctacgg accgggagcg tgcagttgcg aacgcaaggt
     4561 cgaggacagc ctgttttcgc aaaaccgttt catcaccgat cggcgctggc gcggccacgg
     4621 tttcaaattc tggggtgcgg aacaggtgat cttcgaagac aacctcctct ccggtgacac
     4681 ccgcggtctg gtgatgcaga cccatttcgg cgtcaactac cgtaatttca ttgccggcaa
     4741 cacggtggaa cgaactgttc tgggtgagaa tgcgggcgag acgtatctgg ttgagggggc
     4801 gggttatttg ggtgagagtg cggtggagga ggccactccc acgcatgtcc ggattgcgcg
     4861 ctggccaacg gcgcgggggg ctcccgccac ggccgagatg tgcgtgggcc gtttcgttgt
     4921 gatcgcgggc gggcgcggga tcggccaatg gcggcgagtc cgtgcagccg atccggccca
     4981 acgtgtcctg tacccggaaa cgccgtggcg tgtcccgcca gacgcctcca gcgtggtcgt
     5041 gatgatgaac ggcctgatcg acaccgtttt cgtcaacaac caagagaccg attgcggcaa
     5101 gggcttgtac atttacggtg cgggcgcgat caactgcatc atcgaccgtc acctctgcgc
     5161 ccgcactctg ggcattacgt tcaaaacaca tgacgatcgg cagaacgccg acccgatgca
     5221 tcaccaaacg gcacccgact tttttaacct catccgtgat tgccgtgtgc atgacggcgg
     5281 cggactcttc tgcgctgtgg gcggacgtct gccactggaa gaacgcaacg gagtgccttg
     5341 ggccaatttc gccaaccggt tcatcgccaa tgagattcag aacgtgaccc ctttcagcgg
     5401 tgcccagtac ggggcgaact ggacttgggg cggcggatgg gacaacgttc aggcgggcat
     5461 cagtgtgatc cccagcgacc tgggcactgc ggcagggagc ggaagtccgg gccccgaacg
     5521 catccgcgcc gccacgttcc aaaaaaactg gatttcggga tcccgattcg gtgtcggggt
     5581 gagccgtcgc gcggcggaca ccctcctgat gcagaccacg gtttacgatg tggacgtgcc
     5641 gctgacggac aagggccgtg acacccgcga aaaagcaacg gttgtgcgga cacccgaaac
     5701 ctattcgccg gaacggggtc cgatccgttg aggaatccaa acaaacggtt gcccaataaa
     5761 aggtattccg tttatactgt gcccgtttct gggttatctg ataaggagat tcgacatgcg
     5821 aaaccaatgg caaaccaagc aactcgcccg acgttgcgtc atcgccctcc ccttccttct
     5881 gtttctcgtt tcctccggct gcaaaccttc cgcgaaaaga gccgccgaac gcgaggcgta
     5941 cctcaagcat gaagtggagc agacgtccct gcgtattcag gattattcct acgaacggca
     6001 ggatgaggcg ctggttctgg ttgacgaggc gttggccaac cctcgttttg ctggacacaa
     6061 gccgattttc ttcgcgcaca aaatcaacct gctgctctca ctggagcgcg tgaaggaggc
     6121 ctctgatctg gtggttgaag cttggaaaaa agcaccggat caggcgcaag ccgtattcgg
     6181 aaaaatttgc gatttccatc agcagcgcaa cgcataccgc gagatgaacg actggtgcac
     6241 gcgactgctg gatctcggcg cggctttccc gaaagagctg agagggcggt tgatgctgcg
     6301 gcggttgacg gccgcgctgg gcatcaagga catggagcag gcccggagcg atctggacac
     6361 ccttctgacg ctcgtccggc ccgaagagtc tctgccggat ctgcaacgtt ccttggagtg
     6421 gttcattgat atggggaatc accgcgaggc gcttgtcctg ctgaaccatt ttgagaaggc
     6481 tgccgccggt caaccctcgt accaaccgct gatcgtgtcg ctgaaactgc gctgtatcgt
     6541 gctgaccggc gaatggggac gcgtggtcga ggccctcgcg gcctgtgtgc cgcaactgcc
     6601 ggacgcgatg ctgcaccaga ccctcaagaa tgttttcggc gccctgaata aaaacaatca
     6661 gacggatctg ttgcaacagg ccgctaaaca gattttgttc aacgccgtgg gcaaggaggc
     6721 atcggttcac tatgccgccc gcgtgtgggt ggacacgggt atgaccgtcg ataagaaagt
     6781 cctggcggag cggctcaatg tgttgctcaa cgtcaaagtt tccccgattc tggtcggtga
     6841 cttgtacgag cggtattttt acgagacgat cgaggaccac aacgtggtcc ggaacctgtg
     6901 cgcggtgggc gaacgcctgc tgacggtctg cacgaacgag gcgacggtca acatggcgaa
     6961 agtgaaggtg ctcgacggcg cgttcattgt caacgattac gacaaggcga tccacatgct
     7021 cgaacaaggc atccccggca aggataaagc ctggcacgac atgtccctgc ccaaggtcaa
     7081 agcgcaccgt gccatggcgc agaacaagcc gcgcgaagca gtggcgtatt tccgcgcgtt
     7141 catgaatgcc tggctggcga cggatcaaga cgaggaatcc gatccgacta cgggcatctc
     7201 ccacagccgg gaatggattc tcggacgcaa cgccattcgt atcgccaggc tgttggagtc
     7261 cattccagat aaggaggagg cggacaaagc gcgcgaagag gccaaagact atttcaggaa
     7321 ggccctgatc aaagccgaga atgacccgcc ggccctgaaa ctgctgaaag aagagatccg
     7381 gccgctcggt ctataagcgg cgatgatcag cgcggggcgc acggccttca cgggcggggc
     7441 cattcggtca ccagccggac gatcgcctcc agcgcctccg cttcggggag ggttcggatc
     7501 cgttcgcccc gcacaaacag cacgaacacg ccgtcgcccc cggccacagc gatatcggca
     7561 cccttcgcct cgccggggcc gttgaccaca caccccatga ccgccacatg cggccgcggc
     7621 gcgtcagggt gttcgcggta aaaacgttcg aggcggtttt cgattcgcaa cgccagaccg
     7681 gccacgtcca cccgcgtccg gccgcatgtt ggacacgagg tcaccgaagc actcggcgcg
     7741 cgcaacccga cgctgcgtaa gagttccacc cccacgcgca cctcttgggc cggctgatcc
     7801 gtcagcgaaa cgcggatcgt atcgccgata ccctccagca agagtgtgcc gagcgccacg
     7861 caggagcgca ctgttccggg cagaaacgtg ccagcctccg tcacacccag atgaagcgga
     7921 taatcggttc gttccgacaa caggcggtag gcggcaaggg tacgcggcac gtcagaggcc
     7981 ttcaccgaga ttttcatctc gcggtagtcc tcggcctcga gccacgccac atgccgcaac
     8041 gcgctttcca ccagcgcctc cggtgtcgcg gagccgtatc gcgcgagcag atccttttcc
     8101 agcgaaccgc cgttcacacc gatacggatg ggaatgcggt tcggacgggc cgccgccacg
     8161 acggcccgaa cccgttcagg accgccgatg ttaccggggt tgatacgcaa cccgtccgcg
     8221 ccagccgcca gcgccgccag cgccaaccgg tgatcgaaat gaatatccgc caccagcggc
     8281 agcggggagc gcgcacgcac ctgtttgaag gtttcggccg ccgcgtgatc cggcacggtc
     8341 agacgcacca agtcgcaccc gagttctgcg gcttcacaca cctggcgcaa cagggcctcc
     8401 gcatcatgcg gatcggcgtt cgccatggtt tgcacactca ccggggcacc gccgccgatg
     8461 gcgacggacc ccacatgaac ctgacgcgtc gcagtacgcg gacaccgcat acatcgtcct
     8521 cctcatcaac actgcctacc gcggcacgac cgatgcgttg gtctcggacg ccgtctctgc
     8581 ctcctgacgc gcctcgcgaa ccgcggcacg gaccgcccgc gagattttga cctgacgcgc
     8641 gatatcgcgg taaaataaga gcaccatcag tccgatcagt aaaaacgcga acgcgttcac
     8701 cagcgccgaa acgactttcg ggtgcggttt acgacgggtg gtcacttcat acaacgcgaa
     8761 caggatgtgc ccgccatcca gaacgggaaa cggcagcaga ttcaggatcg ccagattgac
     8821 gcaaatcatc cgcaagaatc cgatggcgtc aaggatatcg ccccgtaccg tgtgaaagag
     8881 cccgatcacg atggccaccg gtccgcccac gttacgtgcc accgctttac gctcgccctt
     8941 cgattccggc gccagcaggg cctggagcac gcgcaccacg gacagggagt cccacttcaa
     9001 ctggcgccaa ggattacggt acatcatcca ggcacgaacc cgttccgtgg cgtctttcca
     9061 gacaatgccg acgagatacc ttcccgaggc ctcgtcaaaa cggggtgtca gcacgatctg
     9121 ctggagttcg cggccccgtt tcaccgtaag cgtcacgggt tgcgcccggt tgtgctggat
     9181 cgcttcagcg aactgctggg cctcccgcac cggctctccg ttaatctcca ccaccacatc
     9241 gttcgggagc agtccggctt gaattgccgg catgtcgggc atgagcgccg agacctcgca
     9301 gtgcgttgcg gggaagaccc cttccagcat cttcagttcc acaatctcgc tggtccggaa
     9361 tggcagttcc acctcccgca ccgtgtcccc gcgctggatg ccgaacacgg ctttgccgga
     9421 atcgcccgca agttggcact ccacctgcaa atcggaccag gttcccacct tccggttgtt
     9481 aacgctgagg atccggtccc ccggacgcac cccggctttc caggcttccg agttttccgc
     9541 cacagaaccg atccgcgtat ccagcacgcc tgttttcacg cccggagcca gaaagaggaa
     9601 ataggccaaa aagatggcga gcacgaagtt cccgaccggt ccggccaacg cgaccacaat
     9661 ccgtttccac ggggcgatat cctccaactc cgaacacgaa tccccctccc cgtcccgctc
     9721 gcttccctgc accttctcca tccctgaagg atccaattgc ggcagggcca catatccgcc
     9781 gaacggcacg catcccacct tatacaccac cccgcccacg gtgcgcttcc agagcgcagg
     9841 tccaaatccg attgaaaagg catccacacg caaccccagt cgccgcgcag cgataaaatg
     9901 cccgaactca tgaataaaga tggcgaggct gaaaaacaac gccacgcaaa cacccgatcc
     9961 aatcagactg acccattcca tgcttttcca cctatctcac gtcggcccga tacgccgcaa
    10021 ccggtatgcc gcggcgcggc gcacactcac gttgtccctg atttcttcac ctgtgcgccc
    10081 gcctggcggc gcgcccacgc atccgcttcg agaatggttt ccaactcgac acaggttttc
    10141 acatcatgtt gcgccatcac agcctccacc gcccgggcga tgccggaaaa cggaatcgcc
    10201 ccggccaaaa aggcttccac cgccacctcg tccgcggcat tgagcaccgc aggtgcagtc
    10261 ccccccgcct gtgccgcctc gcgtgccagc cgcagacagg gaaaccgcgt cgtgtccggt
    10321 tcggcaaagg tcaggcatcc catccgggcc aagtccagac ggggcagatc ggcagtcagc
    10381 cgttccggcc aagtcagggc atactggatc ggaaagcgca tatcgggcat ggaaagttgc
    10441 gccagcgtgg caccgtccgt aaacgtgacc atcgagtgca ccacgctctc tggatggatc
    10501 agcacatcga tcttggcgac cggaagattg aacagccagt gcgcctccag aatctccaga
    10561 cctttattca tcatcgtggc cgaatcgatc gtgaccttcc gccccatgtc ccaccgcgga
    10621 tgggtcaggg cctcttcagg cgtgacgcgg tcgaaatccg tttctttgct cgcgccgaac
    10681 ggcccccccg acgcggtcag cgcaaagcgc gcgacacgcg cttcaatgcg ggaggtgtct
    10741 gcggcatccc gtacacaggc cggcggacac cccggggttt gcaggcattg gaacagcgca
    10801 ctgtgttcac tgtccaccgg gagcatccgc acaccagcgg ctgcccgccg ccgcatcacc
    10861 aattcgcctg cggcgaccag cacttctttg gtggccagcg cgatatcgtg ccccactcgg
    10921 atcgcctcca gtaccggttc gaggcccgcc agaccgacca aggcgcacag gaccatatcc
    10981 gccttcggca gcgccgccag cgccgccact cccgcttcac cgggccagac ctcaatcccg
    11041 tgcggacggg ccagcgtctc cgcctcccgc gccgcgtttt catccgcgac cgccaccacc
    11101 ggcacatcaa agcggagggc ttgatccaaa acacgcatga tgtgcgttcg agtcgagagc
    11161 cccgccacac gcaacgcttc gggatgcgcc tcgatcacgc gcaaagcgtt ctcgccgatc
    11221 gatcccgtgc tcccgagtat gaccaccgac ctcataacgc caccgactgt cccacaaacc
    11281 aaaccagata aaaatagagg actgccggcg tgaaggtcag gctgtcgaac atatccagaa
    11341 tcccgccgcc gccacccggc aacaggccag cggaatcctt ggcgttcacc gaccgtttga
    11401 acatggattc aatcagatcc cccagtgcgc cgaccacact gagcaccaaa cccaaaacca
    11461 gcgcgtgggt catactcaac tccgccagcg gaccgcccgg cagacagtcg aaccgcttgg
    11521 cagccgccac cagccccgca ctgaccatca tggagacgac gatcccgccg accaatcctt
    11581 cccaggtttt tttcggcgag acccgcggaa acagtttgtg gcgccccatc gtcaccccaa
    11641 ccgcaaacgc gccaacgtca ctgaacttca ccactgccgc cagataggag gccagaaaga
    11701 cacctgcgcg ggcagaggga atctcgaaca tctcgtccgc tccccactgg gccagccgga
    11761 tgaagaaact caacatgaac ggcaaataaa agaaccccaa cagcgtgact cccacatgct
    11821 cgaccggctt ggcgatccgc gagtcaaaaa gcacgcgcaa caacagggcg gcgaccagca
    11881 tggccagcat cagggtttcg aactggtggc cggtcggcag tcgtcccgcc gcatacggcg
    11941 ggaaggcgta gcaggccgtc aaccacacca ctcccatgac catgccgccg ttccggctca
    12001 ccggatagcc gttgcgcttc atcatctggt aaaactcgtt ctggcaggcg ctcgacatca
    12061 ccagcagcac cccgaacagc atccatcccg gcatggcgca gaggctcaac agccaagcaa
    12121 cgcccaccag cacaccggcg aggattcggc gcatcaacat ggcgcacctc cccctgcccc
    12181 tgccggttgc gccgtcactc cgccaaagcg ccgctcgcgt ttggcatagg cggccagcgc
    12241 cgcttgaaac tccgcctcgc ggaaatcggg ccacagcacc ggtgtgacga aaaactcgct
    12301 gtaggcactc tgccacagca gaaaattgct gatgcgccac tctccgctgg tacggatgat
    12361 cagatcgggg tccggcacat ccggcgcgtc gagccgggcc gccacagcgg cctcatcgac
    12421 ctgttccggc gtaagcgttc ctgcccggac ctcctccgcg atccgccgcg ccgcgcgcgc
    12481 cagctcgttg cggccgctgt aactcaaggc gatgagaagc tggcgttcaa agtgcgccgt
    12541 ttcgcgttcc agacgtgtca gggcctcccg caacggagcc gagagatctt cccgctgtcc
    12601 gatcacccgc agccgcacct gacgcgcgat cagttcccgc tcgtgcttgt ggatgaaggt
    12661 tcccaagagc gccatcag
//
LOCUS       DAOSRB010000071        12677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_4984_length_12677_cov_7.175408, whole genome shotgun sequence.
ACCESSION   DAOSRB010000071 DAOSRB010000000
VERSION     DAOSRB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12677
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4984_length_12677_cov_7.175408"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..824)
                     /locus_tag="PLZ74_05390"
     CDS             complement(60..824)
                     /locus_tag="PLZ74_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97789.1"
                     /translation="MRTSRRLSGGRRAASLCLAFALAGLFCIVAGEAVHHDRTTSGAV
                     GLNIAAGFAIALSLALGLIAVAVQQRQWSRRARNRLVLGFPIALLSLLMLVVDAASRG
                     SARGGLGKAGPSPSALSSPEPVRLAPLFQSGWYGEAHQDGLLLVVTSFHTNSVESRAF
                     NRALRKPVSYATLSVINLGSAAPVVLRRAEVFFLLDSGEKVFIKADRFSQVSITPELV
                     QKLIDLPGEDSVYVGALQKPLLREPTRRRIFGGRRG"
     gene            complement(995..1699)
                     /locus_tag="PLZ74_05395"
     CDS             complement(995..1699)
                     /locus_tag="PLZ74_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOR97790.1"
                     /translation="MATVMLIFLGARDRLLADGVATLAAHAGVPPETVVQLTDLAEQA
                     LPRGREQIRARFRGEEPLVIGCSRPRAVRALLDFAGVDTTARPIEWVALPFDREALRR
                     ESGVPWYPVIDRTLCTGCGTCREYCLFSVYGRTPGEQGVGPVRVVEPLYCKTGCPACA
                     RLCPVGALIFPFCPEAELNGEIETPAPQSNADLKRQLGEDPLRLLTERRKRRGLLDLS
                     KFEQAEEDRIRHSGVL"
     gene            complement(1710..2831)
                     /locus_tag="PLZ74_05400"
     CDS             complement(1710..2831)
                     /locus_tag="PLZ74_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOR97791.1"
                     /translation="MLYWIAPYLTASWGPFRLFSSHLMLLAAGTLAGGIVVWLLLPRL
                     WSRLPTDRGKSLAADGGLKSAGKPTGAGLLLALLLLPVLAVFVPMGRWEAGVVACLYA
                     SMVFGFLDDRSMCPWGEWKKGILDLVVALAAALCLYQGQGSLLWMPFFKSTLTLPPAL
                     YVTGAAALLWFTMNATNCSDGVDGLAGSLTLLSLFALAGFLYGVIGYRPIAEYLLIPH
                     NPDGARWAILVATVAGSLAGYLWHNAEPSRVLMGDAGSRLLGMLVGVAVLVAGNPFLI
                     VVVSPVVLVNGGTGLFKLILLRTLRWAGFDVRPPQTLPQEAAARQLAPVKMLHRIRFP
                     LHDHCRRNLHWSNAQVLMRFVLIQAFLTPLLFVILIKIR"
     gene            complement(2861..3745)
                     /locus_tag="PLZ74_05405"
     CDS             complement(2861..3745)
                     /locus_tag="PLZ74_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOR97792.1"
                     /translation="MQRLEERVQEGVLVCDGAMGTELHRLGLAPGECPEQWCLDHPEK
                     VRAVHQAYREAGSAIVETNTFGGNRYKLAHYGLESAVEAINRAGAALAREVAGDTQWV
                     MASLGPTGVFLEPYGEESEQAFYDAFAAQARAFEAGGADAVVVETMMAVEECCVAVRA
                     VRENTRLTCIASFTFDPQPDGGYASMMGVTPEQFAAQAQAAGAQILGANCGTGTDHMI
                     AIIRRLRAVAGVPLIAMPNAGMPMLVRGETVFPETPDEMAAQVGCLVEAGASIVGGCC
                     GTTPAHIAAIAAALNARR"
     gene            complement(3998..4297)
                     /locus_tag="PLZ74_05410"
     CDS             complement(3998..4297)
                     /locus_tag="PLZ74_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR97793.1"
                     /translation="MAKKATPKTKSEIMAALAAAGEISKKQASTVYEALLKIAYTGAK
                     DPKGIMLPGLGKLIKVKRAARKGRNPATGAEIKIPAKTVVKFRLAKAAKDAVLGK"
     gene            complement(4436..5326)
                     /locus_tag="PLZ74_05415"
     CDS             complement(4436..5326)
                     /locus_tag="PLZ74_05415"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOR97794.1"
                     /translation="MRDTVALYLDHLACERGLAANTRAAYARDLAAFGAFLEDRGITA
                     AGAVTREQVLAFLDSQARLKLSPATRGRRLVALKGFFAFLRAEGIVSSEVTEGMSGPA
                     KGLRLPHTLSEEEIVRLVESVRGTSVYDLRDRAMLELFYACGLRVSEGISLRVEDLRF
                     DESVVWCIGKGDKQRAVPLGSSARQALERYLDGSRPWFARNAAAERHLFLTRLGAPFT
                     RQGVFDMLVKRARAAGVGEAFSPHVLRHCFASHLLAHGAPLRAIQEMLGHADIATTQI
                     YTHIDEGQVVRTHARFHPRH"
     gene            complement(5337..5648)
                     /locus_tag="PLZ74_05420"
     CDS             complement(5337..5648)
                     /locus_tag="PLZ74_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97795.1"
                     /translation="MNRWITLCMLCVMMVTGCRVSDVRTHVVRVPEMAKQEDAEQIRK
                     ALLPLNGLQQDKTVFDLSQRKVTVTYDSMMIAHKNIEIAIAEAGYTANTIPAIRPASS
                     N"
     gene            5736..6506
                     /locus_tag="PLZ74_05425"
     CDS             5736..6506
                     /locus_tag="PLZ74_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97796.1"
                     /translation="MKAFLFLPAVLLLGLLIGAWGPKSDLLDARRKIADLEKRLAAQE
                     KNTRVDTLTRWVQIPDRARAERPRTTDPNRSSLSTNKTANASPVTNAPDTTPPDATTP
                     PDATPQRTAPAPEDLRARIDEAKELWRTRVDIARSQWIARLNLTEAQTPLFDETIDLM
                     NENLYDALQRFADRLESGDPLTPEAGTRVMNEMTAVMVETYDTLASFVPQDRQDETSH
                     MELTDFIDPGVGEPLIAVQDKLRDMPRGTRRHPRGMPR"
     gene            6503..7408
                     /locus_tag="PLZ74_05430"
     CDS             6503..7408
                     /locus_tag="PLZ74_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR97797.1"
                     /translation="MKALRDIGLVAVHEGLDALRSRRALVVLLLYMAAALCNMHWSIN
                     LLHRLENELATALQLPASERAGVISTALWKSKPFQRMMRQMTRNDLVLQDITGKHPAE
                     LIYAFFAFFYTPLLVVLVAGNRIAEEIGSGSVRYTIVRISRAGWSVGKFLGQTMMIGL
                     ALLLSGLGAYAVARFRLAGSGAPGLLLNIVQWSARAWLYSFPFLGLAMGLSHLTRSAS
                     RATVLGILAITLCFVLSILIRHFSGESGWRAMLPAIGMLLPDGYKMCLWRRSVPPLVS
                     GGVTLVTLGFVYLLAGHACFRKRDV"
     gene            7411..8325
                     /locus_tag="PLZ74_05435"
     CDS             7411..8325
                     /locus_tag="PLZ74_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR97798.1"
                     /translation="MDNAIETTGLVKRYGARRALDGFTLSVPRFAVFGLVGPNGAGKT
                     TWMMSVAGLTHLTGGSVNLLGSGAFDPQVHSGRLAILPQDSELPPESRPLELLTHFAR
                     IQGLKRQEAARSATHVLEAVHLGDRLHAPIRSLSHGMRKRVMIAQCFLGYPEVVLLDE
                     PLSGLDPREVENMRGFFTRQRGRQTIVISSHNLHDIERLCTHVAFIDKGRAVRVATLD
                     EVTGRAGKLIYRVTAMPTDRAALEAAVPDTTIAYDAERGEVVCHYNPTLLTAEAVNAR
                     LLPALLAQCGLLAVSRGDSLETEYLRSL"
     gene            8334..8801
                     /locus_tag="PLZ74_05440"
     CDS             8334..8801
                     /locus_tag="PLZ74_05440"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOR97799.1"
                     /translation="MNTPLQIVLLQPQIPHNTGAIGRVCVGLGCALHLIRPLGFHLSD
                     TYVHRAGLDYWNHVRLTVHDSWEAFLDTVQPPRLFFLSTRGARSLYACAFRPDDALIF
                     GNENRGLPKAFYARYGDALFQIPMPGDQSRSLNLANAVSIASYEAYRQLTLPR"
     gene            complement(8785..9216)
                     /gene="tsaE"
                     /locus_tag="PLZ74_05445"
     CDS             complement(8785..9216)
                     /gene="tsaE"
                     /locus_tag="PLZ74_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOR97800.1"
                     /translation="MTPRTHTVRSVEETWRLAAEVAVGLEPGMVIALHGDLGAGKTTF
                     VQGLGQALGIRRPMTSPTFTVSTEYRTPAFTLVHMDLYRLNGPDDLLTIGYPEYLENG
                     AVVAIEWPERAGDLIPADALHVRFTLTGEAETRVIEISAAG"
     gene            complement(9213..10226)
                     /locus_tag="PLZ74_05450"
     CDS             complement(9213..10226)
                     /locus_tag="PLZ74_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR97801.1"
                     /translation="MRTRNVREDIGPIVVYQESAVPYFCLRPRQVARLRAAFPGTRVV
                     WCRTERAFVRNLPTVRVAVTWAFRQAWFEAAPQLRVVISSAAGRDLSPLTPPPRVTVW
                     HGTFHGALMAETVLGWMLAFNRGILSAYRYQIQGALWPREALYGQVRLLHGTHALIVG
                     FGHIGTAIGRLLKPFGVRVTGVRRTPQTRLPAGFGPEDAVVPIGQLDALLPEADHLIL
                     VLPSDTGTDRLINAARLARLPRHAVLYNVGRGNCLDEAALARALTTGRLRGACLDVFA
                     EEPLQPGSPLVADLPGLVRLPHASAFADEYMERFTDEVIARLAARSRRRTRSARAARE
                     GAE"
     gene            complement(10307..12172)
                     /locus_tag="PLZ74_05455"
     CDS             complement(10307..12172)
                     /locus_tag="PLZ74_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR97802.1"
                     /translation="MNGGRRIGILAMTIGCAGMLCGATLMIEAEAFAEKGGWVVDQQF
                     MDQMGSPFLMAHGLGKPVADAVTSCEIPEAGTYRVLVRTRDWAAPWERKDAPGKFRVR
                     INDTLLPQVLGTAGAEWGWHTAGKVTLKKGSARIALCDLTGFNGRCDALILTTEEDFV
                     PESEVARLEAFRRRIGTIRSAQTCETVDLIIAGGGIPGICMAISAARLGLAVALIQDR
                     PVLGGNNSSEVRVHLAGRMNLDPYPRLGDVVSEIGPRRGGNAQPGDYYEDERKIAAVR
                     AEKNIRLYLSTRALGVEKTGARITAVTARHIETGQEIRLAAPLVADCTGDGVIGVLAG
                     AEYRYGRESREETGEPTAVEKADTQTMGSSVQWYSREAEEPVAFPEIAWGLEFNDENC
                     ERVKMGEWTWETGMRLHQVHDFERIRDYGLLVVFSNWSFLKNRSEHKAEYAKRRLDWV
                     AYVAGKRESRRLIGDLVLTEQDIVNQVIYPDATACTTWTIDLHYPDPKNSRHFPGAEF
                     KSIAKHQAIYPYPLPYRCLYSRNVENLFMAGRNISVTHVALGTVRVMRTGGMMGEVLG
                     MAASLCKKHGCTPREVYTDHLGELQELMRRGVGSGREQPPQRYNLGGTLMKVKKE"
BASE COUNT         2469 a         4420 c         3618 g         2170 t
ORIGIN      
        1 accctgctca aaaattgaac ggggtttatt tttgcggtaa tcttacaagc catactttac
       61 tatccacgcc gaccaccgaa gatgcggcgg cgcgttggtt cacgcagtaa cggtttttgg
      121 agcgcgccga cgtaaacgga gtcttcgccg ggcaggtcga ttaatttctg aacgagttcc
      181 ggcgtgatgg agacctgcga gaagcggtcg gctttgatga atactttttc gccggagtcg
      241 agcaggaaaa aaacctccgc acgtctcagc accaccggtg ccgcgctccc cagattgatc
      301 accgagagcg tggcgtagga gaccggcttg cgcaatgcgc ggttgaacgc ccgactctcc
      361 actgaattcg tgtgaaagga ggtgacgacc aacagcaatc cgtcctgatg cgcctccccg
      421 taccaaccag actggaagag cggcgcgagg cggaccggct ccggcgagga caatgccgaa
      481 ggggacggcc ccgctttccc cagtccgccg cgggccgagc cccggctcgc ggcatccacc
      541 accagcatca gcagactcag caacgcgatc ggaaaaccga ggaccagccg gttacgcgca
      601 cgacggctcc attgccgctg ctgcaccgcc accgcgatca gtcccagcgc aagagataag
      661 gcgatggcga accccgccgc gatgttcaga cctaccgccc cggaggtcgt ccgatcgtga
      721 tgcaccgctt ccccggccac aatgcagaag agtcccgcca gcgcaaaagc cagacaaagc
      781 gaggccgccc ttctgccgcc ggaaagacga cgtgacgtcc gcacccctgc gaccgctcgt
      841 ttagaacgct ccgcgcgcac agaattacgg tgatgatgat ggtgatgacc ggatcgcgac
      901 atgtcactcc gtctggtttt gtgataccgt accccaccat gacagagagg tcaattccgt
      961 attcatcccc gaacgcgcgg aacggaacga ccgctcacag cacaccggaa tgacggatgc
     1021 ggtcttcctc agcctgttcg aacttggaca ggtcaagcaa ccctctgcgt ttacggcgct
     1081 cggtcaaaag ccgcaggggg tcttccccca gttgacgttt caaatcagcg ttggactgcg
     1141 gcgcgggcgt ctcgatttcg ccattcagtt ccgcttcagg acagaacggg aagatcagtg
     1201 cgccgaccgg acagagccgc gcgcaggcgg gacatccggt tttgcagtac aacggttcga
     1261 ccacacggac cggacccacg ccctgctccc cgggcgtccg gccatacacg gaaaagaggc
     1321 aatactcgcg gcaggttccg caccccgtac agagcgtgcg gtcgatcacc ggataccacg
     1381 gcacgccaga ttcccgccgg agcgcctcgc ggtcaaacgg caacgcaacc cattcaatgg
     1441 gtcgcgccgt ggtgtcgacc ccggcgaaat cgagcagcgc ccgcacggcg cgcggccgcg
     1501 aacatccgat caccagcggc tcctcgccgc ggaaccgcgc ccggatttgc tcccgcccgc
     1561 gcggcaacgc ctgttccgcc agatccgtaa gctgcacaac cgtttccggc ggcacacccg
     1621 catgggcggc aagcgttgcg accccgtccg ccaacagccg gtcccgcgca cccagaaaga
     1681 tcagcatgac agtcgccaca gagtcctcct taccggatct tgatcagaat cacaaacagc
     1741 agcggcgtaa ggaacgcctg aatcagaacg aaacgcatca gcacctgggc attactccag
     1801 tgcagattcc tgcggcagtg gtcatgcaac gggaagcgga tccggtgcag cattttcacc
     1861 ggcgcgagct gccgcgcagc cgcctcctgc ggaagcgtct gcggcggccg cacgtcaaaa
     1921 ccagcccacc ggagcgtgcg cagcagaatc agcttgaaca gacccgtgcc gccgttgacc
     1981 aacaccactg gcgagaccac cacgatcaga aagggattgc ccgccaccaa cacggccacc
     2041 cctaccagca tacccagcag gcgcgatccg gcgtcgccca tcagcacccg gctcggctcg
     2101 gcattgtgcc acagatatcc ggccagcgaa ccggccaccg tcgccaccaa aatcgcccag
     2161 cgcgcgccat caggattgtg cggaatcagc agatattcgg cgatcggccg atacccgatc
     2221 accccgtaga gaaacccggc gagcgcgaac agcgacagca gggtcagcga accggcaagg
     2281 ccgtccaccc catccgaaca gttcgttgcg ttcatcgtga accacaagag cgctgcggcc
     2341 cccgtcacat aaagcgccgg aggcagggtc agcgtggatt tgaagaacgg catccacaac
     2401 agggaaccct gcccctgata cagacagagc gccgcagcca acgccaccac cagatccagt
     2461 ataccctttt tccactcgcc ccacgggcac atgctgcggt catcgaggaa cccgaaaacc
     2521 atgcttgcgt aaaggcacgc caccaccccc gcctcccaac gccccatcgg cacgaaaacc
     2581 gcaagcaccg gcaacagcaa cagggcgagc aacagccccg ccccggtcgg ttttccagcc
     2641 gatttcaacc cgccgtccgc agccaggctt ttgccccggt cggtcggcag gcgcgaccac
     2701 aggcgcggca acaggagcca cacgactatt ccgcctgcca atgtgccagc ggccaacaac
     2761 atcagatgcg acgagaacag ccggaacggc ccccagctcg ccgtcagata cggtgcgatc
     2821 caatacaaca tcggactttt ctccccctcc cgtttgcgat tcagcgccgt gcgttcagcg
     2881 cggccgcgat ggccgcgata tgcgcgggcg tggtgccgca acagccaccg acaatcgagg
     2941 ctcccgcctc caccaggcac ccgacctgag ccgccatctc atccggcgtt tcgggaaaaa
     3001 ccgtttcgcc cctgaccagc atcggcatcc ccgcgttcgg catggcgatc agcggaaccc
     3061 cggcgaccgc gcgcagacgc cggatgatcg cgatcatgtg atccgtcccc gtcccgcagt
     3121 tcgcgccaag gatctgcgcg ccagccgcct gcgcctgcgc cgcgaactgc tccggcgtga
     3181 cccccatcat cgaggcatag ccgccatccg gctgcggatc gaaagtgaaa ctcgcgatgc
     3241 aggtgagccg cgtgttttcc cgcacggcgc gcacagccac gcaacactct tcgacagcca
     3301 tcatggtttc cacgaccacc gcatccgcgc ctccggcttc gaacgcgcgg gcctgcgcgg
     3361 caaaggcgtc atagaaggcc tgttcgctct cctcgccata cggctccaga aaaacaccgg
     3421 tgggacccag actcgccatc acccattgcg tgtctcccgc cacctcccgc gccagagccg
     3481 cgcccgcgcg gttgatcgcc tcaaccgccg actccaaacc gtaatgcgcg agtttatagc
     3541 ggttcccgcc aaaggtattg gtttccacaa tcgcgctgcc cgcctcgcgg tacgcctgat
     3601 gcactgcccg caccttttcg ggatgatcga ggcaccactg ttcggggcat tcgcccggcg
     3661 caagtcccaa ccggtggagt tcggtcccca tcgccccgtc acataccagc acaccctctt
     3721 gaacccgctc ctccaaccgt tgcatccgca cgcctccccc ttctgccgga cccgaatccc
     3781 ggcggtcgca caataccccg tgtcgcccgc tccgggaaaa ccaccgcctt ccgcccgcat
     3841 cgccatcagc ttagcaaaaa cccgcacaca acataaagcg taatggcaat gggttcgtga
     3901 tgggagagaa cgtgaagatc atgcgtttct gatgaaaaaa gaaagacacc gcatcgaaca
     3961 atgcggtgtc acgtaaagcc atcgcttgtg cgaggcgtta tttgccaagc actgcgtcct
     4021 tggcggcttt cgccagacgg aatttcacaa ccgtcttcgc ggggatcttg atctccgcgc
     4081 cggtggcggg attgcggccc ttacgggcgg cgcgcttgac cttgatcagt ttgccgagcc
     4141 ccgggagcat aatgcccttg ggatctttag cgccagtgta ggcgatcttc aacagagctt
     4201 catacaccgt gcttgcctgc tttttgctga tctcaccagc cgcggccaga gcggccatga
     4261 tttcgctttt cgtcttgggc gttgcctttt tagccatacg ttaactcctt ttcgttaatg
     4321 ggaaaaaccc cacaacagtc cacaacatag ccttttttgt taaagaagca accacaaaaa
     4381 cgaaaaaccc gcgaaaaacc gcaggcaccc cttgatcctt atcgcgcgca accggtcagt
     4441 gtcgcgggtg aaaccgggca tgggtgcgga ccacttggcc ttcgtcgatg tgcgtgtaaa
     4501 tctgggtggt cgcgatatcc gcgtgaccca acatctcctg aatcgcccgc agcggcgcac
     4561 cgtgcgccag caggtgactc gcgaagcagt gtcgcagcac atgcggcgag aacgcctccc
     4621 cgacacccgc agcccgcgcg cgtttgacca gcatgtcaaa cacaccctgc cgcgtgaacg
     4681 gcgcgccaag ccgggtgagg aacagatgcc gctccgccgc cgcgtttcgc gcgaaccacg
     4741 ggcgcgaacc gtccagatag cgttccaacg cctgccgcgc cgaactgccc aagggaaccg
     4801 cgcgctgctt gtcgccttta ccgatgcacc acaccaccga ctcgtcaaac cggagatcct
     4861 cgacacgcag tgaaatccct tccgacacgc gcagtccgca ggcgtaaaac aactccagca
     4921 tggcccggtc gcgcaaatcg tacaccgacg tcccccgaac ggactcgacc aaccggacaa
     4981 tctcctcctc cgaaagcgtg tgcggaagac gcaatccctt ggccggaccg gacatgccct
     5041 ccgtcacctc tgaagacacg atcccttccg cgcggagaaa ggcgaagaac cctttcaagg
     5101 cgacgagccg tcgtccccgc gtcgcggggc tcagcttcag acgggcctgc gagtcgagaa
     5161 aagcgagcac ctgctcgcgc gtgaccgccc cggccgccgt gatcccgcgg tcttccagaa
     5221 acgcgccgaa cgcggcaagg tcgcgcgcgt acgcagcgcg ggtgttggcg gcaagcccgc
     5281 gctcgcaggc gagatgatcg agatacagcg ccacggtgtc gcgcatagcc gcgctcctaa
     5341 ttggaggaag cgggacggat tgccggaatg gtgttcgcgg tatatcccgc ttccgcaatc
     5401 gcgatctcga tgttcttgtg cgcgatcatc atggaatcgt acgtcacggt aaccttgcgc
     5461 tggctcagat cgaagaccgt tttgtcctgc tgcaaaccgt tcagcgggag cagcgctttc
     5521 cggatttgct ccgcatcttc ctgcttcgcc atctccggca cccggaccac atgagtccgg
     5581 acatccgaca cgcggcatcc cgtgaccatc atgacgcaca acatacagag ggtgatccat
     5641 cggttcatgc atgtatcata gccgaaccgg acgcgccccc gcaaacgcaa acttttcgct
     5701 tatcgcccgg tatccgtctg gtgtatcatc cacgtatgaa ggctttcctc tttttgccgg
     5761 ccgtgttgct gctgggactg ctcatcgggg cctggggacc gaaatcggat ctgctcgacg
     5821 cgcgccgtaa aatcgcggac ttggagaaac gcctcgccgc acaggaaaaa aacacgcgcg
     5881 tggacaccct cacgcggtgg gtgcaaatcc cggaccgcgc ccgtgccgag cgcccccgga
     5941 caaccgaccc caaccggtcc tccctttcca ccaataagac ggctaacgcg tctcccgtca
     6001 ccaatgcgcc ggacacaacg ccgccggacg caacgacgcc gccggacgca acgccccaac
     6061 gcacggcacc ggcgcccgag gacctgcgcg cccggatcga cgaggccaaa gagttgtggc
     6121 ggacgcgcgt cgatatcgcg cgctcgcagt ggatcgcgcg ccttaacctg acggaggcgc
     6181 aaaccccgct gttcgacgag acgatcgatc tgatgaacga gaacctctat gacgcactgc
     6241 aacgctttgc cgaccggctg gagtccggcg atccgctcac acccgaagcc ggcacacggg
     6301 tcatgaacga gatgaccgcc gtcatggtcg agacctatga cacgttggcc tccttcgtcc
     6361 cgcaagaccg gcaggacgaa acgagccaca tggagttgac cgatttcatc gatcccggcg
     6421 tgggcgagcc gttgatcgcc gtgcaggaca aactccgcga catgccgcgc ggcacgcggc
     6481 gccacccccg ggggatgccg cggtgaaagc gttgcgcgac atcggactgg tggccgtcca
     6541 cgaggggtta gacgccctgc gcagtcgccg cgcgctggtg gtgctgttgc tttacatggc
     6601 cgccgccctc tgcaacatgc actggtcgat caacctgctg caccggctgg aaaacgaact
     6661 cgccacggca ctgcagttgc cagcgtccga gcgtgcgggc gtgatctcca ccgccctctg
     6721 gaaatccaag ccctttcagc gcatgatgcg tcagatgacc cggaacgatc tggttctgca
     6781 agacatcacc ggcaaacatc ctgcagaact gatctacgcc tttttcgcct ttttttacac
     6841 cccgctgctg gtggtgctgg tggccggcaa ccgcatcgcc gaggaaatcg gctctggatc
     6901 ggttcgatac accatcgtcc ggatctcgcg tgccggatgg tcggtcggta aatttctagg
     6961 gcagacgatg atgatcggac tcgcgctgct gttgagcggg ctcggcgcct atgcggtagc
     7021 ccgcttccgg ttagcgggat cgggcgcgcc cggcctgctg ctcaacatcg tgcagtggtc
     7081 cgcgcgcgcc tggctctatt ccttcccgtt cctcggcttg gcgatgggcc tctcccatct
     7141 gacccgttcg gccagccgcg caacggtgct gggcatcctc gcgatcaccc tctgcttcgt
     7201 gctctcgatc ctgatccggc atttcagcgg cgagagcggc tggcgcgcga tgctgcccgc
     7261 gatcggcatg ctgctgcccg acgggtataa aatgtgcctt tggcgccggt cggtgccgcc
     7321 gctggtgtcc ggcggcgtca cgctggtgac cctcggcttc gtttatctgc tggcagggca
     7381 tgcgtgcttc cgcaaacgcg atgtgtgatc atggacaacg cgattgaaac caccggactg
     7441 gtcaaacgct acggcgcgcg gcgcgcgctg gacgggttca cgctttccgt gccccggttc
     7501 gccgtgttcg gcctggtcgg ccccaacggc gcagggaaaa ccacctggat gatgtccgtg
     7561 gcggggctca cgcacctgac cggcggcagc gtgaacctgt tgggcagcgg cgcgttcgac
     7621 ccgcaggttc acagcggacg cctcgcgatc ctgccgcagg attcggagct gccgccggaa
     7681 agccggccgc tggaactcct gacccacttc gcgcggatcc aaggactgaa gcggcaggag
     7741 gcggcacgat ccgcgacgca cgtcctcgag gcggtgcatc tcggcgaccg cctgcacgca
     7801 ccaatccgtt cgctctcgca cgggatgcgt aaacgcgtga tgatcgccca gtgttttctc
     7861 ggttatccgg aagtggtgtt gctggatgag ccgcttagcg gcctcgatcc ccgcgaggtg
     7921 gaaaacatgc gcggcttttt cacgcggcag cgcggtcgcc aaacaatcgt gatcagttcg
     7981 cacaatctcc atgacatcga acgcctctgc acgcatgtcg catttatcga caagggccgt
     8041 gcggtacgtg tcgccacgct ggacgaggtg accggcaggg cggggaagct gatttaccgc
     8101 gtcaccgcca tgccaaccga tcgcgccgcg ctggaggcgg cggtgccgga tacaacaatc
     8161 gcgtacgatg cggagcgcgg tgaagtcgtc tgtcactaca acccgacact gcttaccgcc
     8221 gaagccgtca acgcgcgcct gctgccagcg ttgctggcgc agtgcgggct gctcgccgta
     8281 tcccggggcg acagtctgga aacggaatat ctccggagcc tgtgacgcaa accatgaaca
     8341 ccccgctaca gatcgtgttg cttcagccgc agatcccgca caacacgggc gccatcggcc
     8401 gtgtatgcgt gggattgggt tgcgccctcc atctgatccg gccgctcggg ttccatcttt
     8461 cggataccta cgtgcaccgg gcggggctgg attactggaa ccacgtccgg ctcacggttc
     8521 acgactcttg ggaggcgttt ctcgataccg tccaaccgcc gcggctcttc ttcctcagca
     8581 cccgtggcgc acgcagcctg tacgcctgcg cattccgccc ggacgacgcg ctgatcttcg
     8641 gcaatgagaa ccgcggcctg ccaaaagcgt tctatgcccg ttacggcgac gcactgttcc
     8701 agatccccat gcccggcgat cagtcccgaa gtctcaacct cgcgaacgcg gtctccatcg
     8761 cctcttacga agcctaccgc caactcaccc tgccgcgctg atttcaatga cgcgcgtttc
     8821 cgcctctccg gtcagcgtga accgcacatg cagcgcgtct gccgggatca ggtcgccggc
     8881 ccgttccggc cactcgattg ccacaaccgc gccgttctcg agatactcgg gatagccgat
     8941 cgtcagcaga tcatccgggc cattgagccg gtagagatcc atatggacca aggtgaacgc
     9001 cggtgtccgg tattcagtgg aaaccgtgaa ggtcggactc gtcatcggcc gccggatccc
     9061 cagcgcctgt cccagcccct gcacaaaggt ggttttcccc gcgcccaaat cgccgtgcag
     9121 cgcgatcacc ataccgggct ccaacccaac cgcgacctcc gcagccagtc gccaggtttc
     9181 ctcaaccgat cggaccgtat gcgtgcgcgg cgtcattccg ccccctccct cgcggcgcgg
     9241 gccgatcgcg tgcgcctgcg gctccgcgcg gccagccggg cgatcacctc atccgtgaaa
     9301 cgctccatat actcgtcggc aaaagcggaa gcgtgcggca ggcgcaccag tccggggaga
     9361 tccgcaacca acggcgaccc cggctgcagc ggctcctcag cgaagacatc gaggcacgcg
     9421 ccgcgcagtc tgcccgtggt cagcgcgcgc gccaacgcgg cctcgtccag acagttgccg
     9481 cggcccacgt tgtagagcac cgcgtgacgc ggcaaacgcg ccaaccgcgc agcattgatc
     9541 aaccggtccg tgccggtgtc cgagggcagc accagaatca gatggtccgc ctcaggcagg
     9601 agcgcatcaa gctgtccgat cgggaccacg gcatcctcgg ggccgaaccc cgcaggcagg
     9661 cgggtttggg gcgtccggcg cacacccgtc acccgcacac cgaacggttt gagcaatctg
     9721 ccgatcgcgg tgccgatgtg cccgaacccc acgatcagcg catgtgtgcc gtgcaacagg
     9781 cgcacctgcc cgtagagcgc ctcacgcggc cagagtgcgc cttgaatctg ataacgatag
     9841 gcagacagaa tcccgcggtt gaaggccagc atccacccca gaaccgtttc cgccatgagc
     9901 gccccgtgaa aggtgccgtg ccataccgtg acgcgcggcg gcggcgtgag cggggaaaga
     9961 tcgcgccccg cagccgacga gattaccacg cgcagttgcg gcgcggcctc aaaccacgct
    10021 tggcggaacg cccacgtcac cgccacccgg acagtcggca ggttccgcac gaacgcgcgt
    10081 tcggtccggc accaaaccac ccgcgttccc ggaaacgccg cgcgcaaccg cgcgacctgc
    10141 cgcggccgca gacagaagta cgggacagca ctctcctgat agaccacgat cggcccgatg
    10201 tcttctcgaa cgttgcgcgt tcgcattttc tctcccgctc cacaggcaat gcctatgggt
    10261 ctccgcgtgc cgtaccgcac gctgttgcgg acggcctgat gcgtaactat tccttcttca
    10321 ccttcatcag cgtaccgccc agattgtacc gttgcggcgg ctgctcccgg cccgacccga
    10381 caccccggcg catcaactct tggagttcgc ccagatgatc ggtatagacc tcgcgcggcg
    10441 tgcatccgtg ctttttgcag agtgaggcgg ccatgccgag cacttcgccc atcatgccgc
    10501 cagtacgcat cacccgcacg gttccgagtg ccacatgcgt cacgctgatg ttccggccgg
    10561 ccatgaagag attctccaca ttgcgggaat agaggcagcg gtacggaagc ggatacggat
    10621 agatcgcctg atgtttggcg atcgacttaa actccgcccc cgggaagtgc cgactgtttt
    10681 tcggatcggg atagtgcaga tcgatggtcc aggtcgtgca ggcggtggca tcggggtaga
    10741 tcacctgatt gacgatatcc tgctcggtca gcacgagatc accgatcaac cggcgcgact
    10801 cccgttttcc ggccacatac gccacccagt cgagacgccg tttcgcgtac tccgctttgt
    10861 gttccgaacg atttttaagg aaagaccagt ttgagaacac caccaacaaa ccgtagtccc
    10921 gaatgcgctc gaagtcgtgg acctgatgca ggcgcatgcc ggtctcccaa gtccactccc
    10981 ccatcttgac ccgctcacag ttctcgtcat tgaactccaa gccccatgcg atctccggaa
    11041 aggcgaccgg ctcctcggcc tcgcgcgaat accactggac cgaggagccc atggtctgcg
    11101 tgtccgcctt ctccaccgcc gtgggttccc ccgtctcctc gcggctttcg cgaccgtaac
    11161 ggtactcggc gcccgccagc acgccgatca cgccgtcacc cgtgcaatcc gccaccagcg
    11221 gcgcggccaa ccggatctcc tgaccggttt cgatatgacg ggcggtcacg gcggtgatcc
    11281 gcgctccggt tttctcaacc cccaacgcac gcgtgctgag atagagccgg atgttcttct
    11341 ctgcccggac cgccgcgatt ttgcgttcat cctcgtaata gtcgcccggc tgggcattgc
    11401 cgccgcggcg ggggccgatc tcgctgacca catcaccgag ccgcgggtaa gggtccagat
    11461 tcatacgccc tgccaaatgg acgcggactt ccgagctgtt gttgccgccc aaaaccggac
    11521 ggtcctgaat cagcgccaca gccaatccca aacgcgccgc cgagattgcc atgcagatgc
    11581 cgggaatacc gccgcccgcg ataatcagat cgaccgtctc acaggtctgc gcactgcgga
    11641 tcgtaccgat ccgccgccgg aacgcctcca gtctcgcgac ctcgctctcc ggcacgaaat
    11701 cttcctcggt ggtcaggatc agcgcgtcac agcggccgtt gaaaccggtc aggtcgcata
    11761 gcgctatccg cgcgctcccc ttcttcagcg tcactttgcc tgcggtgtgc cagccccact
    11821 ccgcacccgc ggtgccgaga acctgcggca acagcgtgtc gttgatccgc acccggaatt
    11881 taccaggggc gtccttgcgt tcccacggcg cggcccaatc acgcgtccgc accagtacgc
    11941 ggtaagttcc ggcctccgga atctcacagg aggtgaccgc atccgcaacc ggcttaccca
    12001 gaccgtgcgc catgagaaaa ggcgacccca tctgatccat aaactgctgg tccacgaccc
    12061 aaccgccctt ttcagcgaac gcttccgcct cgatcatcag cgtggcgcca cacagcatcc
    12121 ccgcacagcc gatggtcatg gccagtatcc cgattcgtct gcctccgttc atctgtcctc
    12181 ccctgctttt ttcgcgccgt acgccaaaaa ccggctcacg gcctttccat acccgcgcac
    12241 attcaaatcc ttgtccgccg tgacacgcgc cacggcaaaa gaccccccgt ctcgaacatc
    12301 ggaatccgtt ccgcatcctg tatccccgca taggggatac aacacaaaca aaaaacgcgg
    12361 ttagtttata ccacctccgc atgcccgtcc agacagcaat gaacgcacgc acaggagacc
    12421 tcacttggca aagcaaatgc gactggggtg gtctcatcca ctacttccga acggggttga
    12481 tgcggccagt ggcatttagt tgctccaagg ggggcggggt cggcggagag ggacgggcgg
    12541 gacagggggg tccgaaggtc atgcgtttca gggtcccatt gcggcgctat cgccatcggg
    12601 atcgggatcg gaatcgccgc cgtcattaca cttgtcgccg cgggccactg cgtgggcgca
    12661 acagtcgatt tcgaacc
//
LOCUS       DAOSRB010000072        12470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5073_length_12470_cov_7.152557, whole genome shotgun sequence.
ACCESSION   DAOSRB010000072 DAOSRB010000000
VERSION     DAOSRB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5073_length_12470_cov_7.152557"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PLZ74_05460"
     CDS             complement(<1..1057)
                     /locus_tag="PLZ74_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOR97803.1"
                     /translation="MAGIILILTISFFVLLLMNAPVAVAIAISSLLAILANGGDAAYT
                     VAQRMANGVDSFPLLAIPFFVFSGYLMGRGGLARRLIDFAALLVGRLPGGLGYVNTLT
                     CMLFGSISGSAAAAVSSIGGFMIPEMNRKGYDRDFNVALTATAATTGLLIPPSNSMIV
                     YSVSAGSVSIAAMFMAGILPGILVGVCLMIAAAVIAIRHGYGKAVPDAEAEPRQNPLI
                     VCWRAIPSLLMMILIIGGILLGIFTATEAAAVSVAYAFVLCVVIYREIPWKDLPGICL
                     QTGITTAVVMLLIGSSSAMSWIMTVANIPQSVSSALMGLSENPVVILLTINALLLVVG
                     MFMDMTPAILIFTPILLP"
     gene            complement(1062..1577)
                     /locus_tag="PLZ74_05465"
     CDS             complement(1062..1577)
                     /locus_tag="PLZ74_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOR97804.1"
                     /translation="MDTLQKIRHGLVRALEVLIIVMVSLLTLTVLWGVFTRFVMGRQA
                     QYTDELARLLLVWVSMIGAALAFGEKAHLGVDFFVNYLHAEARKTLSVVVQFVIIALA
                     LAVFISGGWNLAMAQMGQELPTLRPLTRGMVYLSLPVAGCFISLFALENLVAILRTPA
                     DQIGAQTQSEG"
     gene            complement(1577..2605)
                     /locus_tag="PLZ74_05470"
     CDS             complement(1577..2605)
                     /locus_tag="PLZ74_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOR97805.1"
                     /translation="MNNRSGSMFFAGILTGILIACGLGAFVIRAPRTETGGGGGARTV
                     VLKLAHGLDESHPVHLAMTRMKQRLEELTDGKATMDLYSGGVLGGETDCLEQVQKGEL
                     AMTKVSTAALEAFIPEMKVFSIPFAFRDETHFWNTLHSDIGKKLLAKGAARNFRGLCY
                     YDSGDRNFYSTKKPIRTIDDVKGMKIRVMNSRTAMDMINAMGGSPCPITWGELYTALA
                     QGTVDAAENNPPSFDTSRHFEVCKYFTFSAHQRIPDMVIISTKVWDALPQDVRVALQQ
                     AADESETYQRKLWKEKTGQSLALAKEKGVELITPELDGFRKACAGIIRHKDYAAIVPI
                     YNAIQEVK"
     gene            complement(2623..3936)
                     /gene="gltX"
                     /locus_tag="PLZ74_05475"
     CDS             complement(2623..3936)
                     /gene="gltX"
                     /locus_tag="PLZ74_05475"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOR97806.1"
                     /translation="MSVRVRFAPSPTGNVHIGNIRAAIFNWLFARHEGGRFLLRVEDT
                     DRERSTPEAIRTLLDCMAWLGLDYDEPETYQSQQTERHLALAETLLERGLAYREAKGG
                     GGECVVFRMPKEGRLEFTDLVKGRMRKQAVDTQDFVIVRSNGTPVFHLANVVDDIDMK
                     ITHVIRGDDHVENTFKHIELFRVLGAPVPQYAHLPMIVNQQGKPYSKRDGAAFVGEFR
                     DAGYLPDALFNFLLLLGWAPGDDREVLTREEMVERFTLDRCKSSAARFDLKKLIWMNG
                     EYIRRLPREQFTAEFVRRVEAAGLTTDGTDLPGIIGQMQVRTRFYREIPDSCAYFFTE
                     AYAFDPKAVEKRLRPAGVPELLEEVAAAYAALPEFTPDATHAVLNSLAEARGSGLGAL
                     VHPVRVAVSGLTEGPGLFEMLALLGRDRVCARLRTVATRLRDHQL"
     gene            complement(4017..5771)
                     /locus_tag="PLZ74_05480"
     CDS             complement(4017..5771)
                     /locus_tag="PLZ74_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOR97807.1"
                     /translation="MSNLVQLEINGIPVEVPAGTSILKAAQSVQVKIPTLCYHPDLAP
                     WAACGICVVRSSGSPKLLRACCTDVAPGMKITTHDADIVRVRRTVLELILANHPNDCL
                     QCPRNGNCELQRLAADFGIRDIPFPTDTPSIPVDASTPSIVLNPEKCIKCGRCTQVCQ
                     QMQNVWALEFIGRGEKTRMAPAAEVTLNESPCIKCGQCSAHCPVGAIYERDHTRQVWD
                     ALRNPEKICVVQIAPAVRVAIGEAFGLPPGTLMTGETYASLRRLGFKAVFDTNYSADV
                     TIMEEGSEFIKRFKKGKDLPLITSCCPAWTDWMEKYAQDFMSNFSTAKSPQQMMGVLA
                     KTYYAEKMGIDPAKLFMVSVMPCTAKKFEISRSITMSASGYQDIDVVLTTRELARMIK
                     SAGIDFLNLEKEEADSILGEYTGAATIFGVTGGVMEAALRTAYCLITGEAHPPSIEFE
                     AVRGMEGVKEAVIDIKGTPVRIAVAHQMGNVEQVLDAVREDRKAGRKPRYDFIEVMAC
                     RGGCIGGGGQPTGATDSVRKARTAGIYTDDEKCVIRMSHLNPQVQKLYADYLGAPLTE
                     KAHALLHNHYHPKKPYAK"
     gene            complement(5792..7579)
                     /locus_tag="PLZ74_05485"
     CDS             complement(5792..7579)
                     /locus_tag="PLZ74_05485"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOR97808.1"
                     /translation="MGYKQYILVCGGTACESNQGNALFNALKKAVEKSGLVNEVQVVR
                     TGCLSFCGKGPVVKILPQETFYVEVKPEDAQAIVNEHLVKGRVVSRLLYDKKQSKRNV
                     GIKDIDFYQKQFRVVLRNCGVIDPDKIDEYIARDGYKALEKVLFEMKPQDVVDEVSKS
                     GLRGRGGAGFPTGKKWSFAAMQPAGQKYMVCNADEGDPGAYMDRSTLEGDPHSVLEAM
                     TIAGYAIGADKGFIYIRAEYPLAIQRLENAIAQSREYGLLGSNILGSGFSFDIEIRLG
                     AGAFVCGEETALLASIEGRRGMPTPRPPFPAVKGLWGRPTVINNVETLANIPVILLKG
                     SQWFSKIGTATSKGTKVFALTGKINNSGLIEVPMGTTLREIIFDIGGGIRDGKAFKAA
                     QTGGPSGGIIPPQFLDTPIDYENLAKIGSIMGSGGLIVMDEEDCIPDIAKFYLDFTVD
                     ESCGKCAPCRIGGRKLLNYLDKICKGEGSHEDVQNMQEISEAMTRASLCGLGQTASNP
                     VQSSLRYFADEYREHIEEKKCRAGKCRNLLRYSIDTAKCVGCTACARVCPVGAIAGER
                     KQPHVINSETCIKCGHCFEVCKFHAVARA"
     gene            complement(7595..7984)
                     /locus_tag="PLZ74_05490"
     CDS             complement(7595..7984)
                     /locus_tag="PLZ74_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOR97809.1"
                     /translation="MAKLTLDGLRKLREQKQAAIAKRDPSNKDGQIIVGMGTCGIAAG
                     AKETFDAIIEELKARGITRVQVRQTGCMGLCHAEPMVEVVAPDMPTVIYGQVDAATGR
                     QIVNEHIINKHLIDNHICDKPAMDIVR"
     gene            complement(8142..8585)
                     /locus_tag="PLZ74_05495"
     CDS             complement(8142..8585)
                     /locus_tag="PLZ74_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOR97810.1"
                     /translation="MKTFIAEWKDKPGNLIMVLHRVQQDFGYIPREIAFEVAEALEVP
                     LAKIYGVITFYNFFKLKKPGRHPIQVCMGTACYLKGGEDILLELEGILGVGLNTVTPD
                     GEFSVEAVRCLGCCGLAPVMVIDGEVYGKVKTEDLQGILSRIRKG"
     gene            8888..10162
                     /locus_tag="PLZ74_05500"
     CDS             8888..10162
                     /locus_tag="PLZ74_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97811.1"
                     /translation="MQASLRRRDFLKRGGALAAFNLVPAHVLRSETAPSNQLTRALLG
                     FGGIARSGNHLGYKATRLVALCDPDAQRVNEGIALAEKNGYGKVFACRDFREILARPD
                     VDIVHICTPPHWHGLMSVMAAQAGKDIWCEKPMTRTIGEGRRVMEAVKANNRIFRLNT
                     WFRFQGGFYGFGTEVKPLKKIMENRLLGWPVKAVVGSVTGFSLKFGWSGMVNVTPQPV
                     PETFDYDLWLGPAPFKPYHRHRTHATFRGYWDYDGGGLGDMGQHYLDPVQYLMEKDDT
                     SPIRIEVDCPPQHPDAVGSFRRITFTYADGCQIILDGDDSLKGAPFLEGPKGKVWPKL
                     KSDIPNLDAKLTELPEPLPQQTDFVEAVKLRRPFALNERNGFRSCTIVNLGLVAMRLG
                     RGFRFDPEKLCAVKDPAADKFIYQQMRAPWVM"
     gene            10192..12198
                     /locus_tag="PLZ74_05505"
     CDS             10192..12198
                     /locus_tag="PLZ74_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOR97812.1"
                     /translation="MKTTVLIPLAFVLFAAADLTAQPSAAVWSGQEKEALAGICDDAL
                     ADILRQGAPALEKLFAEVKTGGVSDPLAVTRIAALTQFVMRREGAQSRTAYADALLAA
                     AKRAPEGDVACFFLDQLRWCGLPQQANALREMERSDKPGVAALAAMTVQAVTDDRASK
                     AKPVTPTRCAQLNAEIAALKGKDRTARLFQLFADPDPAIAGVACDWARKDGGRRETER
                     WAAKLDTTTDPLRKTMLLDLLANRNDRRAVGAVAERLCDADNGVALSAHRTLFRLSPS
                     DYAEHLPGVLKTLQPERFVMVRDSARVVPTALLKPALLKAYDGFSDTGKKVAMELFKD
                     RRVSEASRYAIATLNAADAEAAIASYRFLRETAGPDAADTLLQHLLASTGRITPEAQS
                     AYAAAARRDTTGRYLEALTQILKSGTEAQKPLALETAARIGGDTLRQTVENAVADANA
                     DISTAAIRALAEWPEPAALPTLLRLAVNAPDAKRQTLAARGVAKLLNRDGLDKKPYAD
                     LWRTLRGQASEARRKEIDNLFAEEKNVARGKPVTTNVGTQGNNLPAHLVDGTLEKAWH
                     GAKWPAQAQIDLGALHTLRSAHLTFFHDGRRTYTFTLELSEDGKTWKQVADNQAAPKP
                     ARPEGLSLSFAPTPARYVRLNVLKNSVNEAVHVLELKVFEAVFQ"
BASE COUNT         2426 a         4069 c         3629 g         2346 t
ORIGIN      
        1 ccggcagcag gatcggcgtg aagatcagga tggcgggcgt catgtccatg aacatcccga
       61 ccaccagcag cagcgcgttg atcgtgagca ggatgacgac cgggttttcc gagaggccca
      121 tcagcgcgga cgagacggac tgcgggatgt tggccacggt catgatccac gacatcgcgc
      181 tggatgatcc gatcagcaac atcacaacgg ctgtggtgat gccggtttgc aggcagatgc
      241 ccggcagatc cttccagggg atctcgcgat agatcaccac gcacaacacg aaggcgtagg
      301 ccaccgagac ggccgcggct tccgtggccg tgaagatgcc cagcaggata ccgccgatga
      361 tcagaatcat catcagcagg ctggggatgg cacgccagca gacaattagc ggattctgcc
      421 gcggctcggc ttccgcgtcg ggaaccgcct tgccgtatcc gtgacggatc gcgatcaccg
      481 ccgccgcgat cataagacag actcccacga gaataccggg caggatgccg gccatgaaca
      541 tcgccgcgat cgacaccgag cctgcggata ccgagtagac gatcatggag ttcgatggcg
      601 ggatcagcag tccggtggtc gccgcagtgg cggtcagggc cacgttgaag tcgcggtcgt
      661 atcccttgcg gttcatttca gggatcatga agccgccgat ggaggagacc gccgccgccg
      721 ccgagccgga gatcgagccg aacagcatgc aggtgagggt gttgacgtag ccgagtccgc
      781 cgggcagacg tccgacgagc agcgccgcga agtcgatcag gcggcgcgcc aagccgccgc
      841 gccccatcag ataaccggag aagacaaaga agggaatcgc cagcagcggg aagctgtcca
      901 caccgttagc catgcgctgc gccaccgtat aggcggcatc gccaccgttg gcaaggatcg
      961 ccagcagaga cgaaatcgca atcgcgaccg cgaccggcgc gttcatcaac agcaggacga
     1021 aaaaggatat tgtcaggatc aggatgatac cggccatgtc ttcagccctc cgattgggtt
     1081 tgcgcgccga tctgatcggc aggggtcctc aggatggcga cgaggttctc cagtgcgaac
     1141 aaggagatga agcaacccgc gaccggcagc gacaggtaaa ccataccgcg cgtcagcggc
     1201 cgcagcgtcg gcaactcctg gcccatctgc gccatagcca agttccagcc gcccgagatg
     1261 aagaccgcga gcgccaacgc gatgatgaca aactgcacga caacactgag cgtcttgcgc
     1321 gcctccgcat gcaggtagtt gacgaagaaa tcgacaccga gatgcgcctt ttcaccgaag
     1381 gccagcgccg cgccgatcat tgagacccaa accagcagca gacgcgccag ctcatcggtg
     1441 tactgcgcct ggcggcccat cacgaagcgg gtgaagaccc cccaaagcac ggtgagcgtc
     1501 agcagggaaa ccataacgat gatgagcact tccagcgcgc ggaccaggcc gtgccggatt
     1561 ttctgcaggg tgtccatcac ttcacctcct gaatcgcgtt ataaatcgga acgattgccg
     1621 cataatcctt atgccggatg atgccggcgc aggccttgcg gaatccgtca agctcgggcg
     1681 tgatgagttc cacgcctttc tctttggcga gcgccagaga ttgtccggtc ttctctttcc
     1741 agagcttacg ctggtaggtc tcagactcgt ccgcagcctg ttgcaatgcc acgcgcacat
     1801 cctgcggaag cgcgtcccag accttggtgc tgatgatcac catatcgggg atgcgctggt
     1861 gcgccgagaa ggtgaagtac ttgcagacct cgaagtggcg cgaggtatcg aaggaaggcg
     1921 ggttgttctc ggcggcgtcg acggtcccct gcgccagcgc ggtgtaaagc tcgccccagg
     1981 tgatcgggca ggggctgccg cccatcgcgt tgatcatgtc catggcggtg cggctgttca
     2041 tcacgcgtat cttcatgcct ttgacgtcgt caatcgtgcg aatgggcttc ttggtggaat
     2101 agaagttgcg atcacccgaa tcataatagc aaagcccccg gaagttccgg gcagccccct
     2161 ttgcgagcaa tttcttaccg atgtcactgt gcagggtgtt ccagaagtgg gtctcgtcgc
     2221 ggaacgcgaa ggggatgctg aaaaccttca tctccggaat aaaggcttcc agcgcagcgg
     2281 tggagacttt ggtcatggcc agttcaccct tctgcacctg ctccagacag tcggtctcgc
     2341 cgcccagtac gccaccggaa tagagatcca tggtggcctt gccgtcggtc aactcttcca
     2401 gacgctgttt catccgggtc atcgccagat gaaccggatg cgactcatcc aacccgtgcg
     2461 cgagtttgag caccaccgta cgggcgcctc ccccccctcc ggtttccgtg cgcggcgcac
     2521 gaataacaaa ggctcccaat ccgcaggcta tcagaattcc ggtcagtatc ccggcgaaaa
     2581 acattgaacc agaccgattg ttcatctctc atccctttct tttcagagtt gatggtcacg
     2641 caaacgggtc gccacggtcc gcaaccgcgc acagactcga tcacggccca gcaacgccag
     2701 catttcaaaa agacccggcc cctccgtcag accggaaacc gccacgcgca ccggatgcac
     2761 gagcgctccc aatccggacc cgcgggcttc cgccagcgag ttcagcaccg catgcgtggc
     2821 gtccggggtg aactccggca gcgccgcgta ggcggccgcc acctcttcca aaagctccgg
     2881 cacacccgca ggcctgagac gtttctccac cgctttggga tcgaatgcgt aagcctctgt
     2941 gaagaaatac gcgcaggaat ccggaatctc ccgataaaac cgggtgcgca cctgcatctg
     3001 cccgatgatt cccggcagat ccgtgccgtc tgtcgtgagt cccgctgctt cgacccgccg
     3061 gacgaattca gccgtgaact gctcccgcgg caggcggcgg atgtactcgc cgttcatcca
     3121 aatcaatttc ttgagatcga agcgcgcggc gctcgatttg caacggtcca gcgtgaagcg
     3181 ttccaccatc tcctcgcgcg tcagcacttc gcggtcatcc cccggtgccc agcccagcag
     3241 cagcagaaag ttgaagagcg catccggcaa atacccggcg tcacggaatt cgcccacaaa
     3301 cgccgcgccg tcccgcttgg aatacggttt gccctgctgg ttgacgatca tcggcagatg
     3361 cgcgtactgc ggcaccggtg cgccgaggac ccggaacagc tcgatgtgct tgaaggtgtt
     3421 ttcgacatgg tcgtcgccgc ggatcacatg cgtgatcttc atgtcgatgt catccaccac
     3481 attcgccaga tggaagaccg gcgtgccgtt cgaacgcacg atcacgaaat cctgagtgtc
     3541 gacggcctgt ttacgcatcc gccctttcac cagatcggtg aactcaagac gcccctcctt
     3601 cggcatgcgg aaaaccacgc actcgccccc gccgcctttg gcctcacggt aggccaaccc
     3661 gcgttccagc agggtttccg ccaacgcgag atgccgctcc gtctgctgac tctggtaggt
     3721 ttccggctcg tcgtagtcca accctagcca ggccatgcag tcgagcagtg tgcggatcgc
     3781 ctccggcgtg gagcgctcac gatcggtatc ctcgacacgc agcaaaaaac ggccgccctc
     3841 atgccgtgcg aacagccagt tgaaaatcgc cgcccggata tttccgatat ggacattccc
     3901 ggtcggactg ggtgcgaaac ggactcggac agacatgtga acctccttgc tcgggaccct
     3961 ccccctccgc tacggaaagg ccggggttct ccggcctttc cgcatacgcc cgccgcctat
     4021 ttggcgtagg gcttcttcgg atggtagtgg ttgtgcagca gcgcatgcgc cttttcggtg
     4081 agcggcgcgc ccagatagtc ggcatagagc ttctgcacct gcggattgag atgggacatg
     4141 cggatcacgc acttctcgtc atccgtgtag atccccgccg tgcgcgcttt ccgcaccgag
     4201 tccgtcgcgc cggtgggctg gccgccgcca ccgatgcacc cgccacggca ggccatgacc
     4261 tcgatgaagt cataccgcgg cttgcgtccg gccttccgat cctcacgcac cgcatcgaga
     4321 acctgctcca cgttccccat ctgatgggcc accgcgatcc gaacaggggt tcccttgatg
     4381 tcgatcaccg cctctttgac gccctccatc ccgcggaccg cctcgaactc gattgacggc
     4441 ggatgggctt caccggtgat caggcagtac gccgtacgca gcgccgcctc catcacgccg
     4501 cccgtgacgc cgaagatcgt ggccgctccg gtgtactcgc cgagaatcga atcggcctcc
     4561 tctttttcga ggttgaggaa gtcgattccc gcgctcttga tcatgcgggc cagttcacgt
     4621 gtggtgagca ccacgtcaat gtcctgatat ccgctggcgg acatggttat actgcggctg
     4681 atctcgaatt tcttggccgt gcagggcatg accgagacca tgaaaagctt cgcgggatcg
     4741 atacccatct tttccgcata ataggtttta gcgagaacgc ccatcatctg ctgcggcgat
     4801 ttcgcggtgg agaagttgct catgaagtcc tgcgcgtatt tctccatcca gtcggtccag
     4861 gcagggcagc acgatgtgat cagcggcagg tccttcccct tcttgaaccg cttgatgaat
     4921 tcggagccct cctccatgat cgtcacgtcg gcactgtagt tggtgtcgaa aaccgccttg
     4981 aagccgagac ggcgcaggct cgcgtaggtc tcgcccgtca tcagcgtacc cggcggcagg
     5041 ccgaacgcct caccgatggc cacccgcacg gccggcgcga tctgcaccac acagattttt
     5101 tccggattgc ggagcgcgtc ccaaacctga cgcgtatgat ccctctcgta aatcgcgccc
     5161 accgggcaat gcgccgaaca ctggccgcac ttgatgcagg gcgactcatt gagcgtgact
     5221 tccgccgcag gcgccatacg ggtcttctcg ccgcgcccga tgaactcgag cgcccagacg
     5281 ttctgcatct gctggcacac ctgagtacag cgcccgcact tgatgcactt ctccgggttc
     5341 agcacgatcg acggcgtcga ggcgtcgacc ggaatcgacg gcgtgtcggt cgggaacggg
     5401 atgtcccgga tgccgaagtc cgccgccagt ctctgcaatt cgcagttgcc gttgcgggga
     5461 cattgcagac agtcgttggg atggttggcg agaatcagct ccagcaccgt ccgccggacc
     5521 cgcacgatgt ccgcgtcgtg agtggtgatc ttcatgcccg gcgccacgtc cgtacagcag
     5581 gcgcgaagca atttgggcga accggaggat cgcaccacgc agataccgca cgcggcccag
     5641 ggcgcgaggt ccggatggta acagagcgtg gggatcttga cctgcacgga ttgtgcggcc
     5701 ttcagaatcg aggttccggc cggaacctcg accgggatgc cgttgatttc aagttggaca
     5761 aggttagaca tggatgaaac cctttcagaa attaagcgcg cgcgaccgca tggaatttac
     5821 agacctcaaa gcaatggccg cacttgatgc acgtttccga attgatcaca tgcggctgtt
     5881 tgcgttcgcc agcgatggcg ccaaccggac agacccgggc gcaggcggta cacccgacgc
     5941 atttcgcggt gtcgatgctg taacgcagca ggtttctgca tttgccggcc cggcatttct
     6001 tttcctcaat gtgctcccgg tattcgtcag caaagtaacg caggctggac tgcaccggat
     6061 tggaggccgt ctgcccgaga ccgcataacg aggcccgcgt catcgcctcg ctgatctcct
     6121 gcatgttttg gacatcctcg tgcgagccct cgcccttgca gatcttgtcg agatagttca
     6181 gcagtttgcg tccgccgatc cggcagggcg cgcatttgcc gcacgactca tcaaccgtga
     6241 agtccagata gaacttggcg atgtcgggga tacagtcttc ctcgtccatc acgatcaggc
     6301 cgccggaacc catgatcgat ccgattttgg ccagattctc atagtcgatc ggggtgtcca
     6361 gaaactgcgg cgggataatc ccgcccgacg ggccgccggt ctgagccgcc ttgaaggcct
     6421 tgccgtcacg gatgccgccg ccgatgtcga agatgatctc acgcagcgtc gtgcccatcg
     6481 gcacttcgat cagcccggaa ttgttgattt ttccggtcag ggcgaagacc ttggtcccct
     6541 tggatgtggc ggttccgatc ttgctgaacc actggctacc cttcagcagg atgaccggga
     6601 tgttagccag ggtctccacg ttgttgatga cggtgggccg accccacagg cctttgaccg
     6661 ccgggaaggg cgggcgcggg gtgggcatgc cgcgccgtcc ttcgatcgac gcgagcagcg
     6721 ccgtctcttc accgcacacg aacgcgccgg caccgagacg gatctcgatg tcgaacgaga
     6781 acccgctgcc gaggatgttc gatccgagca aaccgtattc ccgggattgc gcgatggcgt
     6841 tttcgaggcg ctgaatcgcc agcggatact cggcccggat gtagatgaac cctttgtcgg
     6901 caccgatcgc ataaccggcg atcgtcatgg cctccaatac cgaatggggg tcgccctcca
     6961 gcgtcgaacg gtccatgtac gcgcccggat cgccctcgtc ggcattgcag accatgtatt
     7021 tctggcctgc gggctgcatc gccgcaaacg accacttttt gccggtcggg aaaccggcgc
     7081 cgccgcgtcc gcgaagtccg gatttggaga cctcgtccac gacatcctgc ggcttcattt
     7141 cgaacaggac cttttcgagc gccttgtacc cgtcgcgggc gatgtactcg tcgatcttgt
     7201 ccgggtcgat cacgccgcag ttgcgcaaca cgacgcgaaa ctgcttctga tagaaatcga
     7261 tgtccttgat tccgacattc cgtttgctct gtttcttgtc ataaagcaga cgcgagacca
     7321 cccgcccctt gacaaggtgc tcgttgacga tcgcctgcgc gtcctcaggc ttgacctcca
     7381 cgtagaaggt ttcctgcggc agaatcttca cgaccggccc ttttccacag aagctcaggc
     7441 aaccggtgcg caccacctga acctcgttga cgagaccgct cttttcgacc gccttcttca
     7501 gcgcgttgaa cagcgcgttg ccctggttgg actcgcacgc tgttccgccg caaaccaaga
     7561 tgtactgttt gtagcccatg atgtgtttcc ccgctcagcg aacaatgtcc attgccggct
     7621 tatcgcaaat gtggttgtcg atgagatgct tgttgatgat gtgctcgttc acgatttggc
     7681 gtcccgtcgc ggcgtccacc tgaccgtaga tcaccgtcgg catgtccggc gcgaccacct
     7741 cgaccatcgg ctccgcgtgg cagaggccca tgcagcccgt ctgccggacc tgcacacggg
     7801 tgatgccgcg ggccttcaac tcctcgataa tcgcgtcgaa ggtttctttc gccccggcgg
     7861 cgatcccgca cgtgcccatc ccgacgatga tctgcccatc cttattcgaa ggatcacgct
     7921 tcgcgatcgc cgcctgtttc tgctcacgca atttgcgtaa accatccaat gtcaacttcg
     7981 ccatgttcca ctcccttgtt tcactgaatc aacgtctgtt cctaaattga cgagaaaagt
     8041 ccgcgccagc gcgaggggtt ccgcgcgttc gaggtcaccg gggcctcccg caagccgctt
     8101 ttggaatcgg cggggagacc gtacactgac gcgcgcagga ctcacccctt ccggatccgg
     8161 gagaggatcc cctgcaagtc ctcggtcttc accttgccgt acacctcgcc gtcaatgacc
     8221 atcaccgggg ccagtccgca acagcccaga caccggaccg cctccaccga aaactccccg
     8281 tccggcgtca cggtgttcag ccccacgccg agaatcccct ccagctccaa gaggatatcc
     8341 tcgccccctt tcagataaca ggcggtaccc atgcacacct ggatcggatg ccgtccgggt
     8401 ttcttgagct tgaagaaatt gtaaaaggtg atgacgccgt agattttggc cagcggcact
     8461 tccagcgcct cggccacttc aaacgcgatc tcacggggaa tgtatccgaa atcctgctgc
     8521 acacgatgca acaccataat caggttgccg ggcttgtctt tccactcggc gatgaaagtc
     8581 ttcatctccg gcgtcagctt ctgaacggct gccgccggtt tcgatgcggt actcacggtc
     8641 tccacctcca cgttcacatt gtcgggatcc cgtaccgtgc cgggtcccgg gggattggtt
     8701 aaaaaaggaa tccggggagt atcgcaaatt tccggatatt ggccaagcaa aaccggtcgt
     8761 tcgaaaaaag cccgccgcac aaaccggtgc ttgccaaccc ttatcccttc cgatatcctg
     8821 attcaattaa gtcaatcaag tagtcggcat gccggctgcg ttgttttttc acatcaggga
     8881 ggggattatg caggcttcat tacgccgccg tgattttctg aaacgggggg gggcgctcgc
     8941 ggcgttcaat cttgtgccag cccatgtttt gcggtcggaa accgcaccca gcaaccaact
     9001 gactcgcgcg ctgctggggt tcggcggcat cgcccgctcg ggcaaccacc tcggatataa
     9061 ggcaacccgt ctggtggccc tctgcgatcc cgatgcccaa cgggtcaacg aggggatcgc
     9121 cctggccgag aaaaacgggt acggaaaggt cttcgcctgc cgtgacttcc gcgagattct
     9181 ggcccgtccg gatgtggata tcgtccacat ctgcaccccg ccccactggc acggactgat
     9241 gtcggtcatg gccgcccagg ccggcaagga catctggtgc gaaaaaccga tgacgcgcac
     9301 catcggtgag ggacgccgcg tgatggaggc cgtcaaagcc aacaaccgta tcttccgcct
     9361 gaatacgtgg ttccgtttcc aaggcggctt ctacggattc ggcaccgaag tcaagcccct
     9421 caagaaaatc atggagaacc gcctgctcgg atggccggtc aaggctgtcg tgggcagcgt
     9481 gaccggattc tccctcaagt tcggatggag cggcatggtg aacgtcaccc cgcaaccggt
     9541 tccggagacc tttgactacg atctctggct ggggcccgca ccgttcaaac cctatcaccg
     9601 ccaccgcacg cacgccacat tccgcgggta ctgggattac gacggcggcg gcctgggcga
     9661 catgggccag cactacctcg acccggtgca gtatctgatg gaaaaggacg acaccagtcc
     9721 gatccgcatt gaagtcgact gcccgcccca gcatccggac gcggtgggtt cgttccgccg
     9781 catcaccttc acttacgcgg acggctgcca gatcatcctg gacggggacg attcactcaa
     9841 gggcgcccct ttcctggagg gcccgaaagg caaagtctgg cccaagctca aatccgacat
     9901 cccgaatctg gacgcgaagc taaccgagtt gccggaaccg ctgccgcagc agaccgactt
     9961 tgtcgaggct gtcaaactcc gccgcccctt cgcgctgaac gagcgcaatg ggttccgctc
    10021 ctgcacgatc gtgaacctcg gcctggtggc catgcgtctc ggccgcggat tccgtttcga
    10081 tccggaaaaa ctctgcgcgg tcaaggatcc cgccgcggac aaattcatct accagcagat
    10141 gcgcgccccc tgggtcatgt gagaccgcat cccatgaaag gattgaaccg tatgaaaacc
    10201 actgtgctga ttccccttgc tttcgtcctg ttcgcggccg ccgatctgac ggcccaaccc
    10261 tccgcggccg tctggtccgg tcaggagaag gaggcccttg ccggcatctg cgacgacgcg
    10321 ctcgccgaca tactccgtca gggcgccccc gccctcgaga agttgttcgc cgaagtgaaa
    10381 accggcgggg tttccgatcc cttggccgtg acccgcatcg ccgccctgac gcagttcgtg
    10441 atgcgccggg agggtgcgca gtcgcgtacc gcatacgcgg atgcgctgct ggccgctgcg
    10501 aaacgcgccc ccgaagggga tgtggcctgc ttcttcctcg accagctccg gtggtgcggc
    10561 ctgccgcaac aggcaaatgc cctccgcgaa atggaacgct ccgacaagcc gggtgtggcc
    10621 gcgctcgccg ccatgaccgt tcaggcggtc acggatgacc gcgccagcaa agcgaagccc
    10681 gtcacgccga cgcgttgtgc gcaactcaac gccgaaatcg cggcgctgaa agggaaagac
    10741 cgcactgccc gcctgtttca gcttttcgcc gatccggatc ccgctatcgc cggtgtcgcg
    10801 tgcgattggg cccggaagga cggcggtcgg cgtgaaaccg aacgctgggc ggcgaaactc
    10861 gacaccacca ccgacccgct ccgcaagacc atgctgctcg acctgcttgc aaaccgcaac
    10921 gaccgccggg ccgtcggagc cgtggccgaa cgactgtgcg atgcggataa cggcgtggcg
    10981 ctctcggcgc accgcacgct tttccgcctc agcccttccg attacgctga acacctgccc
    11041 ggcgtgctca agaccctgca acccgaacgg ttcgtcatgg tgcgcgacag cgcccgggta
    11101 gtgccgaccg cgctgttgaa gcccgcactg ctgaaggcct atgacggctt cagcgatacc
    11161 ggcaaaaagg tcgccatgga attgttcaag gaccggcgcg tgagcgaagc gtcccggtac
    11221 gcgattgcca cgctgaatgc cgcagatgcg gaggccgcca tcgccagtta ccgtttcctg
    11281 cgtgaaaccg ctggcccgga tgccgccgac accctgctcc agcatctgct cgcctcgacc
    11341 ggccggatca ccccggaagc ccaatcggct tacgccgcag ccgcgcggcg cgatacgacc
    11401 ggacgctatc tcgaagccct cacccagatc ctgaaaagcg gcacggaggc acagaagccg
    11461 ctggcgctgg aaacggccgc gcgcattggc ggcgacacgc tccggcagac ggttgaaaac
    11521 gcggtcgccg acgccaacgc ggacatctcg accgccgcga tccgtgcgtt ggccgagtgg
    11581 cccgaacccg ccgccttgcc gacgctgctg cgtctcgcgg tgaacgcgcc ggatgccaaa
    11641 cgccagactc tggcggcgcg cggcgtggcc aaactcctca accgggacgg gctggacaaa
    11701 aaaccctatg cggatctctg gcgtaccctg cgcggccaag cctcggaagc gcggcggaag
    11761 gagatcgaca acctgttcgc cgaagaaaaa aatgtggctc gcggaaaacc ggtgaccacc
    11821 aacgtgggga cccaaggcaa caatctcccg gcccatctcg tggacggcac gctggaaaag
    11881 gcgtggcacg gcgcgaaatg gcccgcgcag gcgcaaatcg atctgggggc cctccatacg
    11941 ctccgatccg cccacctcac tttcttccat gacgggcggc gcacctacac gttcacgctg
    12001 gagctttccg aagacggcaa gacgtggaag caggttgccg acaatcaggc cgcccccaaa
    12061 ccggcccgcc ccgaaggcct cagcctctcc tttgccccga ccccggcccg gtacgtgcgt
    12121 ctcaacgtgc tgaagaacag cgtgaatgag gcggtccacg ttcttgaact caaggttttt
    12181 gaggcggtgt tccaataacc ctaaaaggag cagttgaacg ggtagaattc tagcaaagcc
    12241 ccgtaaacat tgggttttgc cgcagtcccc gccgcatctg tttggcgcag gacgcggcgg
    12301 tgcgcccgat gctctttttc cttagtccca tcccttcgtc gctccacttc gtcgcaagcg
    12361 caatcgacgg agttttggag accaatgcgt atgcgggact aaggtgtccg ccaaccggta
    12421 gggacgcccg cttctcccgt cagaccttat gtcgccccgc gacgggcgac
//
LOCUS       DAOSRB010000073        12237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5194_length_12237_cov_6.628140, whole genome shotgun sequence.
ACCESSION   DAOSRB010000073 DAOSRB010000000
VERSION     DAOSRB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5194_length_12237_cov_6.628140"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="PLZ74_05510"
     CDS             <1..1650
                     /locus_tag="PLZ74_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOR97813.1"
                     /translation="VLEGISDIRDESSSDGIRVVVELKRGAMAQVVLNNLYKHTQLQT
                     TFGANMLAIDQGRPKVMTLKDLFRSYTDHRFEVITRRTQFDLAKAEARKHILDGLLVA
                     LDHLDEVVALIRGSSNREEAKVRLVERFAFSDRQVDAILEMRLYQLTGLERDKVEAES
                     RELCDRIAYLQELLRDPAKIYGLIKQDLAELKAQYVLHARRNDKRRTEIVPLEGEVNI
                     EDLIADEPCVITLSLRGYVKRVPLTVYREQRRGGRGITGVAIKEEDLITTVFVAETHD
                     TLLFFTNFGRVFAERAFEIPEAPRTSFGKPIVNLLQFQEGEQVAALLPIRAFAEDVDV
                     MFATEGGTVKKTALVDYRNINRNGIRALNIGEHDRLIQVRLTRSGDDVLLITANGQAM
                     RFNEKQVRRMGRAATGVRGIRLGAGDAVRSFDVVDDQATLLIATENGYGKRTMFSEFT
                     PHNRGGKGMIAIKDVAGRNGKVVAAHAVREEQSFISITSAGMMIRSPVNDVRLCGRTA
                     QGVRLVRLDDGDTLVSVSIADADTDADIDTDTDADTEETQG"
     gene            1663..2145
                     /locus_tag="PLZ74_05515"
     CDS             1663..2145
                     /locus_tag="PLZ74_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOR97814.1"
                     /translation="MKNWKVLFVKPRTEKKVTEYCRLYGIPFYLPLRERQRIVQRRKI
                     QVRVPIFPGYVFACFPDAQRLALLQTNLLVRILVPINARRLLRELIMVRRALRANPAL
                     KAGPPLTEGRMVRILSGPFMGIEGRVKRLAGTVRVVLNIELIGQAVTVTTDCEQIEPL
                     "
     gene            2200..2679
                     /locus_tag="PLZ74_05520"
     CDS             2200..2679
                     /locus_tag="PLZ74_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOR97815.1"
                     /translation="MDTLLELLKKNARESDATLAKKLALTETEVRERIAAYERDGVIR
                     GYQAVLNQDRIEQTDVRAVIELRIRPEREGGFDRIANRIGKFPQVESMCLMSGAYDLL
                     LFVKGQSLQQVAQFVSAHLASLDGVLSTTTHFILKTYKDQGVLMEGVSHDDRLQVCP"
     gene            2651..3829
                     /locus_tag="PLZ74_05525"
     CDS             2651..3829
                     /locus_tag="PLZ74_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR97816.1"
                     /translation="MTTVSKSAHDFVSPLVRDLPKSGIRDFFDIVASRKDVISLGIGE
                     PDFQTPWHIREKAITSLERGMTRYTSNLGMPELRQELAGYMRDTFRAEYDWQTEILVT
                     VGVSEGFDIAMRAVLSPGDEVLYHEPCFVAYAPLIRMAQGVPVAVETFRRDEFRLTVE
                     ELEKKVTPRTRVLLVSFPNNPTGATLRREDTEALADFVIRHDLLLVSDEVYSELTYEG
                     GRHSLAAIPRLRDRLIFLNGFSKAWSMTGFRLGYVCAPAPLTDAMMKIHQYCMMCASS
                     TAQMAGIEALQHGAADVAAMRESYHLRRNYIVASLNEMGLDCFMPRGAFYVFPSIQST
                     GLDSTTFALRLLNECNVACIPGTAFGACGAGFLRCAYATGMEQLKEAMDRMAAFVQRL
                     "
     gene            complement(3847..5964)
                     /gene="uvrB"
                     /locus_tag="PLZ74_05530"
     CDS             complement(3847..5964)
                     /gene="uvrB"
                     /locus_tag="PLZ74_05530"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOR97817.1"
                     /translation="MEQDITRFNLVSAYQPAGDQPEAIAALSQGLRAGARRLTLEGVT
                     GSGKTFTMANLIREWGRPTLVLSHNKTLAAQLFAELKGFFPDHAVEYFVSYYDYYQPE
                     AYIPQTDTYIEKDASINEEIERYRLGATNALLGRRDVVIVASVSCIYGLGSPEDYREM
                     LVDLTSGCSVARDAVLEQLVGIQYVRNDYEPQPGTFRVRGDSVDIFPSYETDGLRVTF
                     FGDEVESLHTLDPVTGRTGSPLSRVVISPAKHFVMPRAKIDAATSRIACELDARLAEL
                     ERQGKLLEAQRLRQRTDFDLEMLRELGYCNGIENYSRHLAGRVAGEPPACLLDYFEGE
                     FLTILDESHVTVPQLRAMYNGDQARKQTLVEHGFRLPSAQDNRPLRFEEFLDKVGPIV
                     FASATPGPYERQVSEITVEQVIRPTGLLDPPVEIRPLDNQIDNLIEEIRRAAEQGDRV
                     LVTTLTKRSAEDLTAYLTEIGIRVEYLHSDIGAIDRVAILGRLRTGAFDCLIGINLLR
                     EGLDLPEVALVAVLDADKEGFLRSATSLIQTAGRTARHVNGRVILYADRETEAIRKMV
                     DITRTRRQRQEAFNATHGITPQSVRKALNESLDLYAQARHIEERMFAAESSESYDLCR
                     AIADTEAEMIAAADALEFERAALLRDELRELKHLLATPAGPETDGAKSTKPKNRRTRR
                     PQGKGKAAAPPQPAGKRSVRRRP"
     gene            6099..6353
                     /locus_tag="PLZ74_05535"
     CDS             6099..6353
                     /locus_tag="PLZ74_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97818.1"
                     /translation="MKIVRIVFLLVAGIGGARAETIPAPPKGEAVFPAWQAVRDTKTL
                     RALCVPMSGTPQASWEEAGFARPPVRVWRRERRPQDRKRA"
     gene            6418..6570
                     /locus_tag="PLZ74_05540"
     CDS             6418..6570
                     /locus_tag="PLZ74_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97819.1"
                     /translation="MQREWVGNARLITRKLGLSGFTVFNYDTHAEAVLPYLRLGVSAP
                     TGWWPW"
     gene            complement(6592..7842)
                     /locus_tag="PLZ74_05545"
     CDS             complement(6592..7842)
                     /locus_tag="PLZ74_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97820.1"
                     /translation="MTTPRPFGRRAFLRLAHAGMAVLPVMTAKRVSGQSAAVQPATRT
                     YARQVKLGLVGCGGRGSWISNLFKANGGFVMTAVADYFADRAMRVGAQLGVPPSRCFS
                     GLDGYRRLMASGIEAVVLQTPPYFFPAHAEAALDAGLHLYMAKPVAIDIPGTLTIQRL
                     AARATQERRVFLADYQLPRDPVNQEVAKRIRAGALGRLQTVFSSGWAGGNGYQDPPLE
                     TPIANRLKDLVWCNDLALGGDYLVHYDVHILDAVVWALGKKPVAAMGASAALRPSVHG
                     DARDSSTVVFLFDDGTIWCHQGMLGSYHDWLKVGRLTASLQGTQGAACLSYAGKAYVR
                     GGPRHFSGGIPNPEASVKTNIAEFRQAIETSACDNAQVLRAVDSNLTAILGREAAARR
                     DRVTLDTLMREARVLQPDLSGLIR"
     gene            complement(7858..8526)
                     /locus_tag="PLZ74_05550"
     CDS             complement(7858..8526)
                     /locus_tag="PLZ74_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR97821.1"
                     /translation="MPIQVIIVEDDPRLRSGLREIINSDPACACVGAYGSGEEALQQA
                     PALNPQVAVMDINLPGMDGIACVRHLAEILPELQIVMLTVYQDTDNLFRALAAGAHGY
                     LVKPARARDLLDAIRDIHRGGAPMTSALARKVVDAFRDRASPAQPPPAATPPTTESDE
                     LAPREKEVLDLLAQGFSYKEIADKLNVAFATVHTYVLRIYKKLHVRSKNAAVARWLGY
                     QPGE"
     gene            8712..>12237
                     /locus_tag="PLZ74_05555"
     CDS             8712..>12237
                     /locus_tag="PLZ74_05555"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOR97822.1"
                     /translation="MMKNRFSGSGPNLAFFRRIDWTAFWTATIISFLVYFFTLGPSVT
                     LEDSGELAVAGDHLGVPHPPGYPIWTMCAYLFARVFSWVTFRGQPTPAWSISLMSAVF
                     AALAAGITAMLITRSASDMMRDAHEGEEDFRQQESDLLCWAGGVGGSLVFAFSPVMWS
                     QATIVEVYTLNAFFLMWIFLLTYRWMRCPAEKTLWLTAFVFGLGLTNYQVLLLAALPL
                     VVMIFLRDIALFRDFLLVGIPMLLTSHLLQIGAMVPASPGMGGPAFAKAEPLSKVSVP
                     SPGLLVAGCVCVLLGLAVGHGLSWRRNAVSKREPGTGKGGRLDALALQGAWITAGVTI
                     LGALLIFFSTATTRMKEVAATDAPLFDPSVYTSVAALAVLVLAFSLGGAYVFKSRWGE
                     RETLPWLIAAGVSLLLLFFRLSAIRGAAMPAGYMGQPYNWSLPTLVLAGGLVVLFLLA
                     VTLPRGIFYALPVAAVQLAVFVLLRKGGMNGLTHPTSWWFWWPVIWNFVVIGLAWQLL
                     PNGRAVAVTTLFAELGVSFYIYMPIVSDLRNPPMNWGYPRTWEGFKHAITRGQYEKLA
                     PSDVFTKKFLFQLGTYFTDLRVQFTLIAATLGFLPFTLWSFRVGKRRIRAGYVASGLY
                     LLTCVLVLISEFAGGEPLWRLDKLLIGLILFLLVVGGATVVLTQVEAFVKRTWQARNL
                     SEGLTVGISLCGMLAVIALFLAKGLTHALSGTGDPGIGVRLASLALALVILAACGAGW
                     FFFRSFAEKRFDFRSDMDQVTQQWMIATAAGFLVMSVLLVVLANIKGDLQDAFIQKVK
                     FISSHGLFAIWIGYGLAFGLTFGNRILKWLVRLGWLNARLLKPLRIGLVCAGVLVAAI
                     PIYENYTNAYLVFSMSGAEQNGHDFGWQFGNYQLRGAEAITEELEADEEPLPNPLFPP
                     AMEPNAIFFGGTDPGRFVPTYMIYSAQVRPDVFLITQNALADNTYMSTMRDLYGNQIW
                     IPTPDDSARAFQVYVDEVQAGKRPKNAALTIENGRVQVSGALGVMEINGILCDMIFAK
                     NKARHAFYVEESYVINWMFPFMTPHGLILKINPERQAIPAETVRADLDFWDWYVRRLL
                     RNPLFRRDLPGQKSFSKLRSAIAGLYAAHGLRAEAERAFQEARTLYPVSPEANFRLVQ
                     EVLLPQARYVESIRILEEYNRRDPNNERG"
BASE COUNT         2145 a         3535 c         3991 g         2566 t
ORIGIN      
        1 gtgctggagg gtatctccga cattcgcgac gagagttcca gcgacggcat ccgggtggtg
       61 gtggaactca aacgcggcgc gatggcgcag gtggtgctga acaacctgta caaacacaca
      121 cagcttcaga ccacgttcgg cgccaacatg ctggcgatcg atcaggggcg gcccaaggtg
      181 atgacactca aggatctgtt ccggagttat acggatcacc ggttcgaggt catcacccgc
      241 cgcacacagt tcgatctggc gaaagccgag gcgcgcaaac atattctgga cggactcttg
      301 gtcgcgctgg accatctgga tgaggtggtc gccctgattc gcgggtcttc caaccgcgaa
      361 gaggcgaaag tccggctggt ggagcgtttc gccttttcgg accggcaggt ggatgcgatt
      421 ctggagatgc gtctgtacca gttgaccggt ttggagcgtg ataaggtcga ggctgagtcc
      481 cgcgagttat gcgaccggat cgcgtatttg caggaactct tgcgggaccc cgctaaaatc
      541 tacggactga tcaaacagga tctggcggaa ctcaaggcgc agtatgtgct ccacgctcgg
      601 cggaacgaca aacggcgcac ggagatcgtg cctctggagg gtgaggtaaa catcgaggac
      661 ctcattgcgg acgagccgtg cgtcatcacc ctcagcctgc gcggatatgt gaaacgcgta
      721 ccgttgacgg tttaccggga gcagaggcgc ggcggacgcg ggatcaccgg tgtcgcgatc
      781 aaggaggagg acctgatcac cacggtcttt gtcgccgaga cccatgacac cctgctcttt
      841 ttcaccaact tcggccgcgt gttcgcggag cgcgccttcg aaatccccga ggcgccccgc
      901 acctcgttcg gcaaaccgat cgtgaatctg ttgcagtttc aggaaggcga acaggtcgcg
      961 gccctgctgc cgatccgggc atttgcggag gacgtggacg tgatgttcgc caccgaaggc
     1021 ggcaccgtca aaaagaccgc gctcgtcgat taccgcaaca tcaaccgcaa cggcatccgg
     1081 gcgttgaaca ttggggaaca tgaccggttg attcaggtgc ggctgacccg ttccggggac
     1141 gatgtgctgc tgatcaccgc caacgggcag gccatgcggt ttaacgagaa gcaggtgcga
     1201 cgcatggggc gggccgccac cggtgtgcgc gggatccggc tgggggcagg agacgcggtc
     1261 cgtagtttcg acgtggtgga tgatcaggcc acgctgttga tcgccaccga aaacgggtat
     1321 ggtaaacgta cgatgttctc cgagttcacg ccgcataatc gcggcggcaa gggcatgatc
     1381 gccatcaagg atgtggccgg acgtaacggc aaggtggtcg ccgcacacgc cgttcgggag
     1441 gagcagtcct ttatttccat cacttcggcg ggcatgatga tccgctcgcc ggtaaacgat
     1501 gtgcgtctct gcgggcggac ggcccagggg gtccggctgg tccgcttgga tgacggggat
     1561 acgctcgtgt ccgtcagtat cgctgatgcc gacaccgatg ccgatatcga taccgacacc
     1621 gatgccgata cggaggagac gcagggctga cgcggcggga tcatgaagaa ttggaaagtg
     1681 ctgtttgtca agccgcggac ggagaagaag gtcacggagt actgtcggct ttacggtatc
     1741 ccgttttacc tgccgttgcg cgaacggcaa cggattgtcc agcgccggaa aatccaggtc
     1801 cgggtgccga ttttccccgg gtatgtgttt gcctgtttcc cggatgccca gcgcttagcg
     1861 ttgctgcaga ccaacctgct ggtccggatt ctggtgccca taaacgcgcg gcgtctgctg
     1921 cgcgagctga tcatggtgcg gcgggcgtta cgggccaacc ccgcgctcaa ggccggcccg
     1981 ccgctgacag agggccgcat ggtgagaatt ctcagcggtc cgttcatggg gatcgagggt
     2041 cgcgtgaagc gtctggccgg aaccgtacgg gtcgtactca acattgaact gattggccag
     2101 gcggtgaccg tcacgaccga ttgtgaacag attgagccgc tctgacattg cagtccgaac
     2161 ggttttcgct atactgcacc ctcacgaaag gcgagtatta tggatacatt gcttgaactg
     2221 ctgaaaaaga acgcgcgtga atcggacgcg acgctggcca aaaagttggc gctgaccgaa
     2281 acggaagtgc gcgaacggat cgccgcatac gagcgcgacg gggtgatccg ggggtaccag
     2341 gctgtgttga atcaggaccg cattgagcaa acagatgtgc gggcagtcat cgagttacgc
     2401 atccgcccgg aacgcgaggg cgggtttgac cggatcgcca accggatcgg caagttcccg
     2461 caggtggagt ccatgtgtct gatgtcgggc gcctacgatt tactgttgtt cgtcaaagga
     2521 cagagcctgc agcaggtcgc ccagttcgtc agtgcacacc ttgcctcgct cgacggtgtg
     2581 ctttcgacta ccacacactt catattgaaa acatacaagg atcagggtgt cctgatggaa
     2641 ggcgtaagcc atgacgaccg tctccaagtc tgcccatgat ttcgtctccc cgctggtgcg
     2701 tgacctgccg aagtcgggga tccgcgactt tttcgatatc gtggcgagcc gcaaagatgt
     2761 gatctcgctc ggaatcggcg aaccggactt ccagacgcct tggcatatcc gcgagaaggc
     2821 gatcacctcg ctcgaacggg gcatgacacg gtacacctcg aacctcggca tgcccgagtt
     2881 gcgtcaggag ttggccggat acatgcgtga caccttccgg gcggaatatg attggcagac
     2941 agagattttg gtcaccgttg gggtcagcga gggattcgac atcgcgatgc gcgccgtgtt
     3001 atcgcccgga gatgaggtgc tgtaccacga gccttgcttt gtggcctacg cgcccctgat
     3061 ccgcatggct cagggtgtgc cggtcgcggt cgaaaccttc cgccgcgatg agttccggct
     3121 caccgtggag gaattggaaa agaaggtgac gccccgtacg cgggtcctgc tcgtcagttt
     3181 tcccaacaac ccgaccggcg ccacgctacg gcgcgaggat accgaggctc tggcggattt
     3241 cgtcatccgc catgacctgt tgctggtttc ggatgaggtc tattcagaac tcacgtacga
     3301 gggcggacgc cattccttag cggccatccc gcgcttacgc gaccgactga ttttcctgaa
     3361 cgggttttcc aaggcttggt ctatgacagg gttccgtttg ggatatgtct gcgcaccggc
     3421 gccgctgacg gatgccatga tgaagattca ccagtactgc atgatgtgcg cttcgtccac
     3481 ggcccagatg gcgggcatcg aggcgcttca gcatggcgcg gcggatgtgg ccgcgatgcg
     3541 cgaatcgtat catctgcgcc ggaattatat cgtcgcctcg ctcaatgaga tggggctcga
     3601 ttgtttcatg ccgcgcggag cgttttacgt ttttccctcc atccagtcga ccgggttgga
     3661 ttcgaccacc ttcgccctcc gtctgctgaa cgaatgtaat gtggcctgta taccgggcac
     3721 ggcattcggt gcctgcggcg cgggtttcct gcggtgcgcc tacgcgacgg gcatggagca
     3781 gctcaaagag gcgatggacc gcatggcggc attcgtccag cggctctagc gtgtgccatc
     3841 cgcacactac gggcggcggc gcaccgagcg ttttccagcc ggttgcggcg gcgcggccgc
     3901 tttgcctttg ccctgcggac ggcgcgttct gcggttcttc ggtttcgtgg acttggcacc
     3961 gtcggtttcc ggcccggcgg gtgtggccag caggtgttta agttcgcgca actcatcgcg
     4021 cagcagcgcc gcgcgctcga attccaaggc atcggccgca gcgatcatct ccgcctccgt
     4081 gtcggcaatt gcccggcaca ggtcgtagga ttcggacgac tctgcggcga acatccgctc
     4141 ctcgatgtgc cgggcctgag catacagatc cagtgattcg ttgagcgcct ttcggacact
     4201 ctgcggggtg atgccgtgcg tcgcattgaa ggcttcctgc cgctggcgcc gcgtgcgggt
     4261 gatgtcgacc atcttgcgta tcgcctccgt ctcgcgatcc gcatagagga tcacgcggcc
     4321 gttcacgtgc cgtgccgtgc ggcctgcggt ctggatcagc gaggtggcgg aacgcagaaa
     4381 cccctccttg tcggcgtcga gcaccgccac aagcgcgact tcgggcagat cgagaccttc
     4441 acgcagaaga ttgatgccga taaggcagtc gaacgcgccg gtgcgtaacc ggccgagaat
     4501 ggcgacgcga tcaatcgcgc cgatgtcgct gtgcagatat tccacccgga tgccgatctc
     4561 cgtgaggtat gcggtgaggt cttcggcgga acgcttggtc agcgtggtta ccagaacgcg
     4621 gtcgccctgt tccgcggcgc gccggatctc ttcgatcaga ttgtcgatct gattgtcgag
     4681 cggccggatt tcaaccggag ggtcgagcag ccccgtgggg cggatcacct gttcgaccgt
     4741 gatttcgcta acctgacgtt cgtacggtcc gggcgtggcg gaagcgaaca cgatcggtcc
     4801 gaccttgtcc agaaactcct caaagcgcag cggccggtta tcttgggcgg agggcagacg
     4861 gaacccatgc tcgaccagag tctgtttccg tgcctggtcg ccgttgtaca tggcacgcaa
     4921 ttggggcacc gtgacatgcg actcgtccag aatggtgaga aactccccct cgaaataatc
     4981 cagcagacag gcaggcggct cgcctgcgac acgtccggcc agatgccggg agtagttctc
     5041 gataccgttg cagtatccca attcgcgcag catttccaag tcaaagtccg tgcgttgccg
     5101 cagccgttgg gcttcgagca gtttcccctg acgctccagt tcggccagcc gcgcatccag
     5161 ttcgcacgcg atccggctgg ttgcggcgtc aattttcgcc cgcggcatga cgaagtgttt
     5221 ggcgggggag atcaccacgc gcgaaagcgg actcccggtc cggccggtca ccgggtccag
     5281 cgtatgcagc gactccactt cgtcaccgaa gaacgtgacg cgtaacccgt cggtctcata
     5341 agagggaaag atgtcgacgc tgtcgccgcg cacccggaag gtgccgggct gcggctcgta
     5401 atcgttacga acgtattgga tgccgacgag ctgctccagc acggcatcgc gcgcgacgga
     5461 acagccggac gtgaggtcga ccagcatctc acgataatcc tccggtgatc ccagcccgta
     5521 aatgcaggag accgaggcta caatcacaac atcgcgccgt ccgagcaggg cgttggtcgc
     5581 gccgagccgg tagcgttcga tctcctcgtt gatcgaggcg tctttttcga tatacgtgtc
     5641 ggtctgcggg atgtaggctt cgggttggta ataatcataa taactgacga aatactcaac
     5701 ggcgtgatcc ggaaagaacc ctttgagttc ggcgaacagt tgggcagcca gcgtcttgtt
     5761 gtgtgagagg accagggtcg gccgacccca ttcgcggatg agattggcca tcgtgaaggt
     5821 tttgccggat ccggtcactc cttccagcgt cagccgacgc gcacccgcgc gcaacccttg
     5881 cgaaagggcg gcgatcgcct cgggttggtc gcctgcaggc tgatatgccg agacgaggtt
     5941 gaatcgggtg atgtcctgtt ccattgtcac ggtgcgtttc cccgagacag acctttgtcc
     6001 ttgcggaaca tgcggatgat cccgccttgt ggtgcattct tggcttttcg atccattgaa
     6061 ggtaaaatat tatgtgttta ctggatcgga ggcaagggat gaagatcgtt cggatcgtgt
     6121 ttctgcttgt ggcggggatc ggcggggcaa gggctgagac gatccctgcg ccgccgaagg
     6181 gggaggcggt gtttcccgct tggcaagcgg tgcgcgacac caagacgttg cgcgcgctgt
     6241 gcgtgcccat gagcgggacg ccgcaggcct cgtgggaaga ggcggggttc gcacggccgc
     6301 ctgtccgcgt ttggcggcgt gaacgccgcc cacaggaccg gaagcgggca tgattgagat
     6361 caatgtgcgc gacttgattt cacggaggct aacgtcgctt tccgcagttc cgttgccttg
     6421 cagcgggagt gggtcggcaa cgcgcgcctc atcacccgca aattgggctt gtccggcttc
     6481 actgtgttca actacgacac gcacgccgag gcggtgttgc cgtaccttcg gttgggcgtc
     6541 tccgccccga caggatggtg gccgtggtaa ggggccgcgc cgacggcgcg gtcagcgtat
     6601 caggccggag agatcgggtt gtaaaacgcg cgcttcgcgc atcagcgtgt caagtgtgac
     6661 ccgatcccgg cgcgcggcgg cctcgcgccc gaggatcgct gtcaggttgc tgtcgaccgc
     6721 gcgcagaacc tgcgcgttgt cgcaggcaga ggtctcgatc gcctgccgga attcggcgat
     6781 gttggttttg acgcttgcct cggggttggg gatgccgccg ctgaaatggc gcggtccgcc
     6841 acgcacgtat gcctttcctg cgtaggagag acaagccgcg ccctgcgtgc cctgtagcga
     6901 cgcggtgagc cggccgacct tgagccagtc atgatacgat ccgagcatac cctgatggca
     6961 ccagatcgtg ccgtcgtcaa acagaaacac gacggtgctg gagtcgcgtg cgtcgccgtg
     7021 cacggagggc ctgagcgctg ccgatgcgcc catggcggcg accggtttct ttcccaaggc
     7081 ccagaccacc gcatcgagga tgtgtacgtc gtaatgcacc agataatccc cgccgagcgc
     7141 gagatcgttg caccaaacga ggtctttgag ccggttcgcg atcggggtct ccagaggcgg
     7201 gtcctgatac ccgttgccgc ccgcccagcc ggaggagaaa acggtttgca gtcggccgag
     7261 cgcgccggcc cggatccgtt tggcgacctc ctgattcacc gggtcgcgtg gcaactggta
     7321 atccgcgaga aacacgcgcc gctcctgtgt cgcccgggcc gcaagccgtt ggatcgtcag
     7381 ggtaccgggg atgtcgatcg caacgggttt ggccatgtag aggtgcaatc ccgcatcaag
     7441 ggccgcttcc gcatgtgccg ggaagaaata aggaggcgtc tgcagcacta cggcttcgat
     7501 gccggacgcc atcaggcggc ggtaaccatc cagtcctgaa aaacagcgtg acggcggtac
     7561 gcccagttgg gcgccgacgc gcattgcgcg atccgcgaaa taatcggcca cagcggtcat
     7621 cacaaacccg ccgttcgcct tgaatagatt gctgatccac gaaccacgcc cgccgcagcc
     7681 gaccagcccc agtttcactt ggcgcgcata ggtgcgcgtt gctggctgca ccgcagcgga
     7741 ctgtccggag actcgctttg ccgtcatgac cggcagaacg gccatgcctg catgggcgag
     7801 ccgtaaaaac gcgcgacgtc cgaacggtcg gggagtggtc atggttggcg ttttctccta
     7861 ttcgccaggt tgataaccga gccaacgggc cactgcggcg tttttggatc gcacatgcaa
     7921 cttcttgtag atccgcagga cataggtgtg caccgtcgcg aaggcgacat tcagtttatc
     7981 ggcgatctcc ttgtacgaga aaccctgcgc cagcagatcg agcacttctt tctcccgcgg
     8041 cgccaactca tcggattcgg tggtcggggg ggtggcggcg ggcggcggtt gcgcgggcga
     8101 ggcgcggtca cggaacgcgt cgacgacttt acgcgccagt gcgctggtca tgggggctcc
     8161 accacggtgg atatcgcgga tcgcgtccag cagatcccgg gcgcgggccg gtttcacgag
     8221 atagccgtgc gcacccgcag ccagagcgcg gaacaggttg tcggtgtcct gataaacggt
     8281 cagcatcacg atctggagtt cgggcaggat ttccgcgaga tgccgcacgc acgcgatgcc
     8341 gtccatgccg ggcaggttga tgtccatcac cgcgacctgc gggttgaggg caggcgcctg
     8401 ttgcaatgcc tcctcgcccg aaccgtacgc gccgacgcag gcgcaggccg gatcgctgtt
     8461 gatgatttcg cgcaatccgg aacgcagacg ggggtcgtct tctacaatga tcacctgtat
     8521 cggcatgagc gctccctctg tcccgccggg gctgtccggt ccgggaacgg atgtgcccaa
     8581 cgtaacacag ccgccccggt tgttcaagcc gtgttccctc ctcgtggcgc gtataaacgg
     8641 aaagttgtcc ttgccggtga gaggcaagga ttgtaaatta agtgtcttct ttacaagcgt
     8701 gagttgtcgt tatgatgaaa aaccggtttt cgggctctgg ccccaatctt gcttttttcc
     8761 ggcggatcga ctggaccgct ttctggaccg ccacgataat ctctttcctc gtctactttt
     8821 ttacgctggg accctccgtc acactggagg attcgggcga gttggcggtg gccggggatc
     8881 atctgggtgt gccgcatccg cccggttatc ccatctggac gatgtgcgcc tatctgttcg
     8941 cccgggtttt cagttgggtc acgttccggg gacagccgac accggcctgg agcatttcgc
     9001 tcatgtctgc ggtgttcgcc gcgttggcgg ccggcatcac ggcaatgctg atcacgcgtt
     9061 cggcttccga catgatgcgg gacgcgcacg agggcgagga ggatttcaga cagcaggaga
     9121 gtgacctgct ctgctgggcc ggcggtgtgg gcggcagtct ggttttcgcg ttcagcccgg
     9181 tgatgtggtc gcaggccacg atcgtggagg tgtacaccct caacgctttt ttcctcatgt
     9241 ggatctttct gctgacctat cgctggatgc ggtgcccggc ggaaaaaacc ttgtggctga
     9301 cggctttcgt gttcggcttg gggctgacga attatcaggt gctgctgctg gcggccctgc
     9361 cgctggtggt gatgattttc ctgcgtgaca tcgcactgtt ccgcgatttt cttctggtgg
     9421 gcatccccat gctgttgacc tcccatctgt tgcagatcgg agcgatggtg ccggcctcgc
     9481 ccggtatggg cggccccgcg tttgctaaag cggagccgct ttcgaaagtt tccgtgccgt
     9541 ctccgggtct gctggttgcg ggttgcgtct gtgtgttgct ggggttggcg gtgggccacg
     9601 ggttatcttg gcgccgaaac gcggtgagca agcgcgagcc cggaaccggc aagggggggc
     9661 ggctggatgc gctggctctt caaggcgcat ggatcacggc gggcgtgacg attctgggtg
     9721 cgctgctgat tttcttttcc accgcgacga cccggatgaa ggaggtcgcg gcaaccgatg
     9781 cgccgctgtt cgatcccagt gtgtacacca gcgtggctgc gttggccgta ctggtgctgg
     9841 ccttcagttt aggcggcgcg tatgtcttta aaagccggtg gggcgagcgg gaaaccctgc
     9901 cgtggctgat cgccgcaggc gtatcgcttt tgttgttgtt tttccggctc tctgccatcc
     9961 ggggcgcggc gatgcccgcc ggatacatgg ggcagccgta taactggtca ttgccgactc
    10021 tggtgctggc aggaggattg gttgtcctgt ttctgctggc ggtcacgttg ccgcgcggca
    10081 tcttctacgc cctgccggtg gcggcggtgc aactggccgt gtttgtgctg ttgcgcaaag
    10141 gcggcatgaa cggactgacc catccgacct cctggtggtt ctggtggccg gtgatctgga
    10201 atttcgtggt gattgggctg gcctggcaac tgcttcccaa cgggcgggcg gtggctgtga
    10261 ccacgctgtt cgcggagttg ggggtctcct tctacatcta catgccgatc gtttcggatc
    10321 tgcgtaaccc gccgatgaac tggggatacc cgcgtacgtg ggaaggtttc aaacacgcca
    10381 tcacccgcgg tcagtacgaa aaacttgccc cgtccgatgt gttcaccaaa aagttcctct
    10441 tccaactcgg cacctacttc accgacttgc gtgtgcagtt cacgctgatc gccgccacgc
    10501 tgggcttttt gccctttacg ctttggagtt tccgggtcgg gaaacggcgc atccgcgcag
    10561 ggtatgtcgc cagcggcctg tacctgttga cctgcgtgct ggtgctgatc tccgagtttg
    10621 ccggcggtga accgctctgg cgtctcgaca agctgctgat cggactgatc ctgtttctgc
    10681 tggtggtggg gggggcgacc gtggtgctca cgcaggtgga agcgtttgtg aaacgcacct
    10741 ggcaggcgcg caacctctct gaagggctga ctgtggggat ctcgctgtgc gggatgctgg
    10801 cggtgatcgc gctttttctc gccaaaggcc tgacccatgc gctttccggc acgggcgatc
    10861 ccggcatcgg ggtccggctg gcttcgctgg cgctggcgct ggtgatcctt gccgcctgcg
    10921 gggcgggctg gtttttcttc cgttcctttg cggaaaagcg ttttgatttc cgttccgaca
    10981 tggatcaggt gacccagcag tggatgatcg cgaccgcagc ggggtttctg gtcatgagtg
    11041 tgcttctggt ggtgctggcg aacatcaagg gcgatttgca ggacgccttt atccagaagg
    11101 tcaagttcat cagttcgcac gggctgttcg cgatttggat cggctacggt ctggcgttcg
    11161 gactgacctt cggcaatcgc attctgaagt ggctggtgcg gctcggctgg ctcaatgccc
    11221 gtctgctgaa gccgttgcgc atcggtctgg tgtgtgcggg tgtgcttgtg gccgcgattc
    11281 cgatctacga gaactacacc aacgcgtatc tggttttctc catgagcggt gcggagcaga
    11341 acgggcacga cttcggttgg cagttcggca actaccagtt gcgcggtgcg gaggcgatca
    11401 ccgaggagtt ggaggcggac gaggagccgc tgccgaatcc gctgtttcca cctgcgatgg
    11461 agcccaacgc gatctttttc ggcggtacgg atcccggccg gtttgtgccg acatacatga
    11521 tttactcggc ccaggtacgg ccggacgtgt ttttgatcac gcagaacgcc ttggctgaca
    11581 acacctacat gtcgaccatg cgcgatctct atgggaacca aatctggatc cccacgccag
    11641 acgacagcgc gcgcgccttt caggtttacg tggatgaggt gcaggccggt aagcgtccca
    11701 agaatgcggc cttgacgatt gaaaacggcc gggtgcaggt gagcggcgcc ttgggcgtga
    11761 tggagatcaa cgggatcctg tgcgacatga tcttcgcgaa aaacaaagcg cgccacgcgt
    11821 tttacgtgga ggagagttat gtgatcaact ggatgtttcc gttcatgacg ccccacggtc
    11881 tcatcctgaa gatcaaccct gagcgtcagg ccattcctgc ggagacggtg cgggctgatc
    11941 tggatttctg ggattggtat gtgcggaggc tgttgcgtaa tccattgttc cggcgcgatc
    12001 tgcccggcca gaaatcgttc tccaaactgc gtagcgccat tgcgggcctg tatgccgcgc
    12061 acggcctgcg cgccgaggcg gagcgggcct ttcaggaggc gcgcacgctc tatccggtca
    12121 gtcccgaggc caatttccgc ttggtgcagg aagtcctgtt accccaggcg cggtacgtcg
    12181 agtcgatccg gattctggaa gagtacaacc ggcgtgaccc caacaacgaa cgcgggt
//
LOCUS       DAOSRB010000074        12220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5204_length_12220_cov_7.817756, whole genome shotgun sequence.
ACCESSION   DAOSRB010000074 DAOSRB010000000
VERSION     DAOSRB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5204_length_12220_cov_7.817756"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1257)
                     /locus_tag="PLZ74_05560"
     CDS             complement(127..1257)
                     /locus_tag="PLZ74_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOR97823.1"
                     /translation="MNVQRSTLNAQRSSENRGSALIIALWTIALLSLLVMSFALDALL
                     EGRVNLYVRQRRQVDYLTQSGIVIAEMLLLEYRNATASTTTDEKEDRWLGAKLDLQKG
                     EASTGDVVVDAANPDGGVVRVKISAGESSRWPINLLFHTGAGGAQVDKIWENVLTVAN
                     VPQEYWEELIDCWTDWVDADNTVTGRNGAERDYYEGLEQPYTARNGPIDTVDELLKVK
                     GFLPAIVNGGVLNPEEKDPKKQIVIRSGGIKAFFDIYGELVKINVNSADKEVLLTVPG
                     IDGDELLAGAIVEERETGSRRVSSAATDLDRESKLFKDWNDLTARIEGIPLTASDFLS
                     YAPEKYFKIEVEGEAGGISHRIEAMAIVEGDKVRYVRWREDP"
     gene            complement(1254..2138)
                     /locus_tag="PLZ74_05565"
     CDS             complement(1254..2138)
                     /locus_tag="PLZ74_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR97824.1"
                     /translation="MMRRDRNGFTLLELTLAIMILSIMMLLSFFCFDAVVQSWNAGLE
                     MSDSLAQADYVMEQIVSGLRSAYYPHAGIQTEQYGFQHTDGGEGEEATDTISWTKIGR
                     ALVGERTQLAEVPHRVSLSVEQASREHDGGLMAKVWRDALQLEDFDPEKDVKPFLLAP
                     RVIGLDCRILKEPPERNATELDWEDTWSESNTIPYKVSLTLYMRPAKEREAPLAVTRD
                     VEIPLWDISQNPVKQGRGAGTGSRDAPGGRSRGGQQPIPPARGGGTQSGARSATGGGR
                     PGGGGPPGGGGPPGGPMQ"
     gene            complement(2170..2622)
                     /locus_tag="PLZ74_05570"
     CDS             complement(2170..2622)
                     /locus_tag="PLZ74_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR97825.1"
                     /translation="MRRNGFTLIEILIATMILAFGLVSLMVGLGHCAQMMMLSKTYQD
                     AQFVFSLGERLYPVPPADEIQDAEDDEKLNIDPVTVEEMAAELDEDLPRERLKALEGF
                     TFERHVDEREEPETNVVDDKLFVLRTRVSWGSGKEGYYEELVRFIRPK"
     gene            complement(2622..3236)
                     /locus_tag="PLZ74_05575"
     CDS             complement(2622..3236)
                     /locus_tag="PLZ74_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR97826.1"
                     /translation="MRRGFTLVELLVVIVIIGVMALMIGPTFTTGSDMTRMRTASRGV
                     MQMARYARTMAVLYQTPMVLVIASDGGLRVEQGQGGKGAPPVSVASDASGQSAESGGA
                     SYMMTDLDAEKVYERVRFRVALDEECLDGDELGMEKLEREESWAGEGDAEEAVVRVRI
                     PFESNGRCLPFTVRVFAAGDADEADVLTVRVDRFGVARIVGDEA"
     gene            complement(3331..3732)
                     /locus_tag="PLZ74_05580"
     CDS             complement(3331..3732)
                     /locus_tag="PLZ74_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOR97827.1"
                     /translation="MKKERDEKRRRSRRGGFTLVELLLVVCILGILAAVVIPNIMGHD
                     EEARRQATRTSITAIEQAVQIFAMRHNGKLPDSLDELTVGTDDAPGLLKEGALNDSWG
                     TPFTYTKTGKKFKIVSAGPDGEVGTDDDLTN"
     gene            complement(3782..5029)
                     /locus_tag="PLZ74_05585"
     CDS             complement(3782..5029)
                     /locus_tag="PLZ74_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOR97828.1"
                     /translation="MASFSYKALTKDGRKVEGVLEAGDRRAALVAVERLGYTPISVME
                     SKTRKAKAKGVPFWKLKLGSGEQRMKPVEVLLFTSELSDLLEAGMTLGAALNCLANQG
                     EEDSPQSRIASDLRDRILRGEPFSDAVTAHPATFPPLYGNMIRAGEASGAMIEVLRRL
                     VEHYERNENMRSKITSALTYPTVVLIFGVLTVIFAMVKVIPQFAKIFEGMGTALPTPT
                     LILVGMSDFVVKYGVFLVAAIGIGGVMFKRYLKTDTGRLWWDGLKLKAPLIRGIVACG
                     AYSSLAYTMKTLLANGVNVLQALRIAEETCGNALIAEALRNARQRVTDGTTISGPLAA
                     SKVFPRMMTDMLAVGEQSGDMPSSLGHIGRRYENEMDRNIKIFTNALEPILIVLIGGV
                     VGFVAVSILMAVFKVTSSLSPAG"
     gene            complement(5173..6867)
                     /locus_tag="PLZ74_05590"
     CDS             complement(5173..6867)
                     /locus_tag="PLZ74_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOR97829.1"
                     /translation="MPGQAEALAALMLKKTGLFTDTQISSVLAADHSDGTGVAGAVVR
                     LGLAREEDFLRKIGEMLGFGYTDLEVTRPTDEAITRLPARAVYQYNVLPLSVENGVLT
                     VVTCDPFNTGMTDGLRLAAGCPVRVTLSPREMIDKAIRKYYGVGADAIEKMIEDGRYE
                     VDADLASISKIDVNEMGAEASIVKFVNQIIAEADRQGATDIHVEPMEDELRIRYRIDG
                     MLHKVDVPPQLNRLKAAILSRIKVMANLDIAEKRLPMDGRIGVRLNNDDIDIRVSTMP
                     TAYGESISLRLLQKAGNFVKLSDLGFNDFDYQHVVKLINRPNGIFLVTGPTGSGKSTS
                     LYAFLHEINKIQVRILTAEDPIEYEMPGINQVLVRQDIGLTFARALRAFLRQDPDIIM
                     VGEIRDGETAEIAINASLTGHLVFSTLHTNDAAGAFARLVDMGAEPFLVASAVAGVMA
                     QRLVRRLCPVCKKEIPLDRKWYDLPYPPAKETVWVPEGCDACTRTGYRGRGGLFELLV
                     VTEDIESAIIGRRSSGEIRDIAIGHGMKTLREDGWLKVFKGMTSVEEVMRVTEDVT"
     gene            6984..8063
                     /locus_tag="PLZ74_05595"
     CDS             6984..8063
                     /locus_tag="PLZ74_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97830.1"
                     /translation="MAYSTAMNPIPPKHTPGSTGEDTPETGGGLVAMYHAASQANAES
                     FPVLKAFQDYLEAERSRARRRVTQLSVFFAVLMGVVVSGFLTAGIFMWRNMAAVQNKL
                     LDVALATREPAPQPLPQPAQPPAVSPILEESVRQMSRATVALQDNLDRKLNGVSVLTE
                     KVHARVASQDSELEKLRGEMRSMLEQSAKLKDELVTLRNNVNKPAPPQVAAPAPAQPA
                     PPPPAQPPAPAVAAKPAASVSAPPVPAAPAPAAPAPATPAPAAPAVAAKPAASVSAPP
                     VPAAPSASAPASAVTAAKPAAEKPAAAPHSARDARFPPAVKAPPVTPAGVTAPDAPHG
                     MVVGTLPIRTKNLGNLRWHVLLPAE"
     gene            8118..8678
                     /locus_tag="PLZ74_05600"
     CDS             8118..8678
                     /locus_tag="PLZ74_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOR97831.1"
                     /translation="MNTARSPSPRDPLRRGPHGGLVLFVCTGNTCRSPMAEALFRAAV
                     PPDSSWRAASAGLAAEEGKPMSAPARVVLAEMAGCVDFADHRSQPVTGKLVADARMIV
                     VMTGAHARQLTARFPEARDKLALLRAFDSAAPAYCDLDDPFLGSPDAYRACRDHIRAA
                     LPGLIRYLTEGGGSCLPSSAIHQETP"
     gene            8675..9133
                     /locus_tag="PLZ74_05605"
     CDS             8675..9133
                     /locus_tag="PLZ74_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOR97832.1"
                     /translation="MILDTLDSSFRYETLHPLFPSAFAFLRRPDLDALACGRIDLAGD
                     DALYALIQTYETQPPAEGKPEAHRRHLDIQYVVSGSECVAYAPLAGLTPCQPFDEAGD
                     YGLYTGSTSLVRLSQGMFAIFYPNDAHLPCRQDGHAAPVRKIVIKVKVSE"
     gene            9237..10049
                     /locus_tag="PLZ74_05610"
     CDS             9237..10049
                     /locus_tag="PLZ74_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOR97833.1"
                     /translation="MSVKTMLFVDGSWLYHSRQVLFESLGEESGFEIDYKRIPDIIAH
                     EIADVLDAEVDVVRTNYFGTIPVNKQGYNPSKQKAFYDFLALQCAYDTEILEIDFRRE
                     PQARPDDKWVNVALASSMLYFAAVPGAYDVATLVGGDADYIPLLKRVRAMGKRVQVVG
                     MNNIDGKFLTSAMLLTTSGIQDLPPIFLDEHAQDIRLVREEQTRACKNCGCEERTTWA
                     GPDFFCSNCRSEHRKQIRVCDTCGCEEETTWDKPFFYCAACRSKYREGENNS"
     gene            10061..11686
                     /locus_tag="PLZ74_05615"
     CDS             10061..11686
                     /locus_tag="PLZ74_05615"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOR97834.1"
                     /translation="MTLPVTFLPTGKTVRVPCGTSLLQAAVQAGLLIDTPCGGSGTCG
                     KCRMRIRAGEVSAGGSSQHLTAEQLEAGWRLACVTRVTGPLEVEVPGTSLLARLDTIL
                     VNGAPVSIRRDPHRLGDMGVAFDLGTTTVAGTLFDLHTGLERASHAVMNRQVRMGDDV
                     ISRIHAVRQDPSTLTQLQTAAIETLNEILDALCDACGETSGTVRRVTVAGNTAMQQLL
                     LGIDPSPLGEAPFTAAFTDAQTTGAARLGLKVAANAMLTVFPQIGGFVGGDTVAGLLA
                     SRFDTLTHPALLVDIGTNGEIALFTGNALLVASSAAGPAFEGARIRQGMRAIPGAIDQ
                     IWMQDGDLRCHVINDAAPCGLCGSALVDVVSVLLQAGLIDETGLLAPPAAAPQETCRA
                     LHDRLIPFEDAWACVLARDACGEPLVMLTQKDIRELQLAAGAIRTGIEVLLARANLTP
                     RDLDALLLAGGFGNYIRREHALRIGLLPPVHELAVRFVGNASLSGAKRALLAQAELDR
                     AQQLRNRATHVELAADPRFSELFMQHMSFPAGS"
     gene            complement(11775..>12220)
                     /locus_tag="PLZ74_05620"
     CDS             complement(11775..>12220)
                     /locus_tag="PLZ74_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97835.1"
                     /translation="AIGIGIDCFTTEHFLGFQGFWFQGFRVSSGSGSLSGSGSIVSPQ
                     NTFFGFKVSGFKVSWFQGFWVSGFMVSGFMVSSGSGSLSLSGSTVSPQPHSLTAFEVA
                     GFARISIRQPCDAGADGPESGAARWRGGFPANRRRSILLLLGLIL"
BASE COUNT         2358 a         4224 c         3334 g         2304 t
ORIGIN      
        1 gttcatttcc tgctgcccgc ccttcacact agagcggccc gcttcccggc agggacggtt
       61 ccccgaaccg tccgcggacg cctcggggag gcgtccctac cctcaagcac gcacgaatgc
      121 acttcctcat gggtcctccc gccatctcac gtagcggacc ttgtcgccct ccacaatcgc
      181 catcgcctca atccggtggg agatcccacc cgcctccccc tccacttcga ttttgaagta
      241 cttttcagga gcgtaactca ggaaatcgct cgccgtcagg ggtatccctt cgatacgggc
      301 ggtcaaatcg ttccaatcct tgaacagttt gctctcccgg tcgagatcgg tggccgcgga
      361 ggaaacacgc cgactacccg tctcgcgctc ctccacaatc gctcccgcca gcaattcatc
      421 cccatcgatg cccggaacgg tcaacaacac ctctttgtca gcggaattga cgtttatttt
      481 aacaagttcc ccatagatgt caaaaaaagc cttgatgccg ccggaccgga taacgatctg
      541 ttttttgggg tctttctcct ccgggttgag cacacccccg ttgacgatgg ccggcagaaa
      601 ccccttgacc ttcagcaact cgtcaaccgt gtcgatcgga ccgttacgcg cggtgtaggg
      661 ctgctccaac ccctcatagt aatcgcgttc cgcaccgttg cggccggtga cggtgttatc
      721 cgcatcgacc cagtcggtcc agcagtcgat cagctcctcc caatactcct gcggcacgtt
      781 cgccacagtc agcacgtttt cccatatctt gtccacctgt gcgccgcccg ccccggtatg
      841 gaagagcagg ttgatgggcc agcggctgga ctccccggcc gagatcttga cccgcaccac
      901 gccaccgtcc gggttcgccg cgtcgaccac cacatcgccg gtggaggctt cgcctttctg
      961 gagatccagt ttggcaccca gccaacggtc ttctttctca tccgtagtgg tcgaagcggt
     1021 agcgttccgg tactcgagca gcaacatctc cgcaatcacg atgcccgact gggtcaaata
     1081 atccacctga cgacgctgcc gcacatacag gttcacgcgg ccctccaaca gggcatccag
     1141 cgcaaaggac atcaccagca gactcagcag ggcgatcgtc caaagcgcga taatcagagc
     1201 cgagcctctg ttttcactgg agcgttgagc gttgagcgtt gagcgttgaa cgttcactgc
     1261 atcggccctc ccggcggccc gcctccgcca ggcggcccac ccccgccagg cctgcctcca
     1321 cctgtcgcgc tacgcgcacc cgattgtgtt cctccacccc gcgcaggcgg aatcggctgc
     1381 tgtccgccac gcgaccgccc tcccggcgcg tcgcggctgc ctgtgcccgc ccccctcccc
     1441 tgtttgacgg gattctgcga gatatcccac agcgggatct ccacatcgcg cgtaaccgcc
     1501 aacggagcct cacgctcttt tgcgggccgc atatagaggg tgagcgaaac cttgtaggga
     1561 atggtgttcg attcggacca cgtgtcctcc caatcgagtt ccgtggcgtt ccgctcgggg
     1621 ggctctttca ggatccggca gtccagaccg atcacccgcg gcgccaacaa gaacggtttc
     1681 acatccttct ccgggtcgaa atcctccaat tgcagggcat cgcgccacac tttcgccatc
     1741 aggccgccgt catgctcccg gctggcctgc tcgacggaaa ggctcacacg atgcggcacc
     1801 tcggccagtt gcgtccgctc gcccacaagt gcccggccga tcttggtcca actgatcgta
     1861 tcggtcgcct cctccccctc gccgccgtcc gtatgctgga atccgtactg ctccgtctgg
     1921 atgcccgcat gcgggtaata ggctgaacgc aatccggaaa cgatctgttc catgacataa
     1981 tcagcctgcg ccagggaatc ggacatctcc aatcccgcat tccaactctg caccacggca
     2041 tcgaaacaga aaaacgacag gagcatcatg atcgacagga tcatgatggc cagagtcagc
     2101 tccagcagtg tgaatccgtt tcggtctctg cgcatcatgt gccggacacc ctctccggcc
     2161 cctcctccct catttcggcc ggatgaaccg caccagctcc tcgtaatacc cctctttacc
     2221 gctaccccag gagacgcgcg tccgcaacac gaacagcttg tcgtccacca cattggtttc
     2281 cggctcctcg cgctcgtcca catgccgctc aaaggtgaac ccttccagcg ctttgaggcg
     2341 ctcacgcggc agatcctcat ctagttcggc cgccatctcc tccacggtga cggggtcgat
     2401 gttgagtttc tcgtcatcct ccgcatcctg aatctcgtcg gccggcggga ccggatacag
     2461 gcgctcaccc aaactgaaca caaactgcgc atcctgatac gttttggaca acatcatcat
     2521 ctgcgcacaa tggcccaagc ccaccatcag gctcaccaac ccaaacgcga ggatcatcgt
     2581 ggcgatcaga atctcgatca gggtgaatcc gttgcggcgc atcacgcttc atcccccacg
     2641 atccgcgcga ccccgaaccg gtccacccgg acggtcagca catctgcctc gtccgcatcc
     2701 cccgcagcaa acacgcgcac ggtgaacggc agacaacgcc cgttgctttc aaaggggata
     2761 cgcacccgca cgacagcctc ctccgcatcc ccttccccag cccacgactc ctcccgttcc
     2821 aatttctcca tccccaactc atccccgtcg agacactcct catccagcgc cacacggaac
     2881 cgtacccgct cataaacctt ttcggcgtcc agatccgtca tcatatacga cgctccgccg
     2941 ctctcggccg actgcccaga cgcgtcagac gcaacggaaa caggcggcgc tcccttcccg
     3001 ccctgcccct gctccacgcg cagcccgccg tccgatgcga tcaccagcac catcggcgtc
     3061 tgatacagca cagccatcgt gcgggcgtac cgcgccatct gcatgacgcc ccgggacgcc
     3121 gttcgcatgc gggtcatgtc cgaacccgtc gtgaacgtcg gcccgatcat cagagccatc
     3181 actccgatga tcacgatcac caccagcagt tcgaccagtg tgaatccgcg ccgcatgcgc
     3241 ctcctcaaga cccgaatcga tttgaatcga tctgaatcga tccgggtcga atcacgccga
     3301 agcgggtcga ttcgagtcgc ctttcccgca ctagttcgtg aggtcgtcat ccgtaccgac
     3361 ctcaccgtcc ggtccggccg acacgatctt gaacttcttg ccggtcttgg tataggtgaa
     3421 cggcgtcccc cacgagtcgt tgagcgcgcc ttccttgagc agtcccggcg cgtcgtcggt
     3481 cccgacagtc agttcgtcca gcgagtccgg cagcttgccg ttgtggcgca tggcgaaaat
     3541 ctgtaccgcc tgctcgatcg ccgtgatgct cgtgcgcgtg gcctgacgcc gcgcctcctc
     3601 atcatgtccc atgatgttcg ggatcaccac cgcggccaga atgccgagaa tacagaccac
     3661 cagcagcagc tcaaccaacg tgaacccgcc ccggcggctc cgcctgcgtt tttcatctct
     3721 ctcttttttc attgcgctcc ctcctttgtg atgtctgact atcatcaaaa atccccgcct
     3781 cctaccctgc cgggctgagc gacgaggtga ccttgaacac tgccatcaaa atgctgaccg
     3841 ccacaaaccc gaccacccca ccgatcagca cgatcagaat cggctccaac gcattcgtga
     3901 aaatcttgat gttccggtcc atctcgttct cataccgccg accgatatgc cccaatgaac
     3961 tgggcatatc ccccgactgc tcgccgaccg ccagcatgtc cgtcatcatc ctggggaaaa
     4021 ccttactggc ggccagcggc ccggaaatgg tggtgccgtc cgtcacccgc tgccgcgcat
     4081 tacgcagcgc ttcggcgatc agggcgttgc cgcaggtctc ttccgcgatc ctgagggctt
     4141 gcagaacatt gacgccattc gccagcagcg tcttcatcgt atatgccagg ctggagtaag
     4201 ctccacaagc cacgatgccc cggataagcg gcgccttcaa tttaaggccg tcccaccaga
     4261 ggcgccccgt atcggtcttc agatagcgct tgaacatcac ccccccgata ccgatcgccg
     4321 ccaccagaaa cacgccatac ttaacgacga aatcgctcat gccgaccagt atcagcgtgg
     4381 gtgtcggcaa cgccgtgccc ataccctcga agatcttggc aaactgcggg atcaccttga
     4441 ccatggcgaa aatcaccgtg agcacgccga agatcagcac caccgtcgga taggtgagcg
     4501 cggaagtgat cttcgagcgc atgttctcgt tacgctcata atgctcgacc agacggcgca
     4561 gcacttcgat catcgcgccg gaggcctccc ccgcgcggat catgttgccg tacagcggcg
     4621 ggaaggtggc cggatgcgcc gtgacggcat ccgaaaacgg ctcgccgcgc agaatgcggt
     4681 cgcggaggtc cgaagcgatc ctgctctggg gagaatcctc ctcgccctgg ttcgcaaggc
     4741 agttcagggc cgcaccgagc gtcatgcccg cctccagcag atcactcaac tcggaggtga
     4801 acagcaacac ctcgaccggc ttcatccgct gctcgccgct gcccagcttg agtttccaga
     4861 aaggcacgcc cttggccttg gccttccggg tcttggattc catcacggag atcggagtat
     4921 acccaagccg ttcgaccgcc acgagcgcgg cacggcggtc gcccgcctcc aacaccccct
     4981 caaccttgcg gccgtctttc gtcagcgctt tgtaggaaaa actcgccatg cacccagtct
     5041 ctgtcagtta ggagttagga tttaggagtt aggaattggg ggaaccgagc cttctcaact
     5101 cctaaatcct cactcctaaa tcctcactcc tcactcctaa atcctaattc ctaaatccta
     5161 aatcctaaat cctcacgtca cgtcttccgt cacccgcatg acctcctcaa ccgaggtcat
     5221 gcctttgaac accttgagcc atccgtcctc acgcagggtc ttcattccgt gcccgatcgc
     5281 gatgtcgcgg atttcgccgg aggagcggcg accaatgatg gccgactcga tgtcctccgt
     5341 caccaccagc agctcaaata acccgccgcg tccgcggtac ccggtacgcg tgcaggcatc
     5401 acacccttcc ggtacccaga ccgtctcttt tgcgggcgga tacggcagat cataccactt
     5461 ccggtccagc ggaatctctt ttttgcacac ggggcaaagg cggcggacca agcgctgcgc
     5521 catcacgccg gccacggcgg acgccaccag aaacggctcg gcccccatat ccaccagacg
     5581 cgcaaacgcg cccgccgcat cgttggtgtg cagcgtgctg aacacgaggt gtcccgtcaa
     5641 cgacgcattg atcgcgatct ccgcggtttc gccgtcgcgg atttcaccca ccatgatgat
     5701 gtccgggtcc tgacgcagaa acgcgcgcaa cgcccgcgcg aaggtcaggc cgatatcctg
     5761 ccgcaccagc acctgattga tccccggcat ctcgtattcg atcggatcct cggccgtcag
     5821 aatccgcacc tggatcttgt tgatctcgtg caaaaaggcg tagagcgagg tggacttccc
     5881 tgaaccagtc ggcccggtga ccagaaaaat gccgttgggc cggttgatga gtttcaccac
     5941 atgctggtaa tcgaaatcgt tgaacccgag gtcgctcagt ttgacgaagt tcccggcctt
     6001 ctgcagcaga cgcagggaga tcgactcccc gtacgccgtc ggcatggtgg agacgcggat
     6061 atcgatatcg tcgttgttga gccgcacccc gatgcgcccg tccatcggca gccgcttctc
     6121 ggcgatgtcc agattcgcca tcaccttgat gcgcgagagg atcgcggcct tgagccggtt
     6181 caactgcggc ggcacgtcca ccttatgcag cataccgtca atccggtacc ggatgcgcaa
     6241 ctcgtcctcc atcggctcca catgaatgtc cgtggcaccc tgacggtccg cctccgcaat
     6301 gatctggttc acgaacttga cgatcgacgc ctcggcgccc atctcgttca cgtcgatttt
     6361 ggagatcgag gcgaggtcgg catccacctc gtagcgccca tcctcgatca tcttctcgat
     6421 cgcgtccgcc cccacgccgt agtatttgcg gatcgctttg tcgatcattt cgcgcgggct
     6481 gagggtgacc cgcaccggac aaccggccgc cagtcgtaac ccgtccgtca tcccggtgtt
     6541 gaacgggtcg caggtgacca cggtaagcac cccgttctct accgacaggg gcagcacgtt
     6601 gtactggtat accgcgcgcg cgggcagacg ggtgatggcc tcatccgtcg gccgggtgac
     6661 ctccagatcc gtatagccaa acccgagcat ctcgccaatc ttacggagga agtcctcttc
     6721 acgcgccaat ccgaggcgca cgaccgcacc cgccacaccc gttccgtccg agtgatcggc
     6781 cgccaacacg ctgctgatct gcgtgtcagt gaagagcccc gtttttttca acatgagggc
     6841 agccaaagcc tctgcctgtc cgggcatgag cacatccctt tctgtgaact ggtccgattc
     6901 agaaacgtaa aggataagtg tacaccaaag ccgacgcgca atccatgcct aatctgattt
     6961 ttcccctttc cggaaaaccc caaatggcgt actctactgc catgaacccg attcccccaa
     7021 aacatacgcc cggttccacg ggcgaggaca cgcctgaaac cggaggcggt ctggtcgcca
     7081 tgtatcacgc ggcctctcag gccaacgccg aatcgttccc cgtattgaag gcgtttcaag
     7141 actatctgga ggccgagcgc tcgcgggcgc gccgccgcgt cacacaactc tccgtctttt
     7201 tcgcggtctt gatgggggtg gtggtctccg ggttcctcac cgccgggatc tttatgtggc
     7261 gtaacatggc cgcagtgcag aacaagcttc tcgatgtggc gctggccacg cgggaacccg
     7321 cgccccaacc gttaccgcag ccagcccagc cgccagccgt ttcgccgatc ctcgaggaat
     7381 cagtgcgcca gatgtcgcgc gccaccgtgg cgttacagga caatctggac cggaaactga
     7441 acggcgtgag cgtcctcacc gaaaaggttc atgcccgggt cgcctcgcaa gacagcgaac
     7501 tcgagaaact gcgaggagag atgcgctcca tgctcgaaca gagcgcgaaa ctcaaagacg
     7561 aactcgtcac cctccgtaac aacgtgaaca agcccgcgcc gcctcaggtt gccgcaccgg
     7621 caccggccca gcctgcaccc cctccgccgg cccagccgcc agcccctgcc gttgccgcga
     7681 aaccagcggc gagcgtttcc gcgccccccg tacccgcagc ccctgcacct gcggcccctg
     7741 cacctgcaac tcctgcaccc gcagcccccg ccgttgccgc gaaaccagcg gcgagcgttt
     7801 ccgcgccccc cgtacccgca gccccgtccg catccgcacc agcttcggcg gttaccgcgg
     7861 caaaacccgc cgcagagaaa cccgctgccg cgccgcattc cgcgcgtgac gcgcggtttc
     7921 cgccagccgt gaaagcgccg ccggtcacgc ctgcaggcgt gaccgcgccg gacgcgccgc
     7981 acggcatggt tgtcggcacg ttgccgatcc ggacaaaaaa cctcggtaac ctgcgctggc
     8041 atgtgctgct ccccgctgaa tagggcgact tcgtaatgtc tccgcacgtc cataacttaa
     8101 agggggaacg ttgggcgttg aatacggcgc gctccccttc cccgcgcgat cctttgcggc
     8161 gcggcccgca cggcgggctc gtcctctttg tctgcaccgg caacacctgc cgcagtccca
     8221 tggcagaggc gttgttccgt gccgccgtac cgccggattc gtcttggcgc gcggcttccg
     8281 caggactggc cgcggaagag ggcaagccga tgtcggctcc ggcgcgtgtc gtgctcgccg
     8341 aaatggcggg ctgcgtcgat ttcgcagacc accggagcca gccggtcacc ggaaaactcg
     8401 tggccgacgc gaggatgatc gtggtgatga ctggcgcgca cgcgcgccaa ctcacagccc
     8461 gcttccccga agcccgggac aaacttgcgc tgttgcgcgc gtttgattcc gcagcccccg
     8521 cgtattgtga tttggatgat ccgtttctgg gctcccccga cgcgtaccgc gcctgccgcg
     8581 accacattcg ggcggccctg ccgggactca tccgttacct gacggaaggg ggcggttcct
     8641 gcctgccgtc gtctgccata catcaggaga caccatgatc cttgacacgc tggattcgag
     8701 tttccggtac gaaaccttgc acccgctttt tccctccgcc ttcgcgtttc tgcggcgtcc
     8761 cgatctcgac gcgctggcct gcggccgtat cgacctggct ggcgacgacg cgctttacgc
     8821 cctgatacag acatatgaaa cccaaccgcc cgcagagggc aaaccggaag cgcaccgccg
     8881 ccacctcgac atccagtatg tcgtgtccgg cagcgagtgc gtcgcctatg ccccgctggc
     8941 cgggctcacc ccctgccagc cgtttgacga agccggggat tacggcctct ataccggttc
     9001 cacctctctc gtgcgactct ctcaaggtat gtttgccatc ttctacccga acgatgcgca
     9061 cctgccctgc aggcaggacg ggcatgccgc gccggtacga aaaattgtca tcaaagtaaa
     9121 agtttcggaa tagacacccg cgccgggttt tggcattctg tgcctgtcct gatcgacgca
     9181 cgccgaaaac gcaaggtgtg cggggaacat agcgaactca agaaaggtaa ttacgcatgt
     9241 cggtgaaaac aatgctcttt gtcgatggca gttggctgta tcacagtcgg caggtgctct
     9301 tcgagtcatt gggggaagaa agcggattcg agattgatta caaacggatt ccggatatca
     9361 ttgcgcatga gatcgccgac gttctggatg ccgaagtcga tgtcgtccgt accaactatt
     9421 tcggcaccat cccggtgaac aaacagggtt acaacccctc caaacaaaag gccttctacg
     9481 attttctggc cttacagtgc gcgtatgaca cggagatcct cgagatcgat ttccgccgtg
     9541 agccgcaggc ccggccggat gacaaatggg tcaacgtggc gctggcctcc tcgatgctct
     9601 atttcgcggc cgtgcccggc gcatatgacg tggccacgct ggtcggcggc gacgcggact
     9661 acattccgtt gctgaaacgt gtgcgcgcca tgggcaagcg ggttcaggtc gtcggcatga
     9721 acaacatcga cggcaagttc ctcaccagtg ccatgctgct gaccacttcc ggtattcagg
     9781 atctgcctcc gattttcctc gatgagcacg cgcaggatat ccggctcgtg cgggaagagc
     9841 agacgcgcgc ctgcaagaac tgcggatgcg aggaacgcac cacatgggcg ggaccggact
     9901 tcttctgctc caattgccgc agcgaacacc gcaaacagat acgcgtgtgc gacacctgcg
     9961 gatgcgagga agagacaacc tgggacaagc cgttcttcta ttgcgcggct tgccgcagca
    10021 agtaccgcga aggcgaaaac aactcctgac tccagtcgtg atgactcttc ccgtcacgtt
    10081 tctgccgacc ggcaaaaccg tgcgtgtccc atgcggcacc tccctgcttc aggcagccgt
    10141 tcaggcggga ctgctgatcg acaccccatg cggcggcagc ggaacctgcg gcaaatgccg
    10201 gatgcggatc cgcgccgggg aggtttcggc gggcggctcg tcgcaacacc tgaccgcaga
    10261 acaacttgag gccgggtggc gactcgcctg cgtgacgcgc gtgaccggcc cgctggaggt
    10321 ggaggttccc ggaacctcgc tgctcgcccg gctggacacc attctcgtga atggtgcgcc
    10381 ggtgtcgatc cgccgagacc cgcaccgact cggtgacatg ggcgtggcat tcgatctcgg
    10441 caccaccacc gtggccggca ccctgttcga tctgcacacc ggtctcgaac gcgcgtcgca
    10501 tgcggtgatg aaccgccaag tccggatggg tgatgatgtg atcagccgga tccacgcggt
    10561 ccgccaggat ccctccaccc tgacgcaact ccaaaccgcc gcaatcgaaa ccctgaacga
    10621 aatcctcgac gcgttgtgcg acgcctgcgg tgaaacgtct gggaccgtcc ggcgggtgac
    10681 ggtcgcgggc aataccgcca tgcagcagtt gctgctggga atcgatccct ccccgctcgg
    10741 cgaggcaccg tttacggcgg ccttcaccga tgcgcagacc acgggtgcgg cccgtctcgg
    10801 actcaaggtc gcggccaatg ccatgctcac cgtctttccg caaatcggcg ggttcgtcgg
    10861 cggtgatacg gtggccggcc tattggcatc ccgcttcgac acgctcacgc atccggccct
    10921 gctggtggac atcggaacca acggggagat cgcgctcttc acgggaaacg cgttgctggt
    10981 ggcttcttcc gcagccggtc cggctttcga aggcgcgcgg atccgtcagg gcatgcgcgc
    11041 catccccggc gcgatcgacc agatctggat gcaggacggt gacctgcgtt gtcacgtgat
    11101 caacgacgcg gccccgtgcg ggctctgcgg ctcggcgctg gtggatgtgg tgtcggtctt
    11161 gctgcaagcc ggcttgatcg atgagacggg cctgctggcc cctcctgccg ccgcaccgca
    11221 agagacctgc cgcgcgctgc acgaccgcct catccccttc gaagacgcct gggcctgtgt
    11281 gctggcccgg gatgcctgtg gtgaaccgct tgtgatgctg acacagaaag atatccgtga
    11341 actgcaactc gcggcaggcg cgatccgcac cggcatcgag gtgctgctgg cccgcgcgaa
    11401 cctcacgccg cgcgatctcg acgccctgct gctggctggc gggttcggca attacatccg
    11461 gcgcgagcat gcgctgcgca tcggcctgtt gccgccagtg cacgaactcg ccgtccggtt
    11521 tgtcgggaac gcttcgctga gcggcgcgaa acgcgcactg ctcgcacaag ccgagctgga
    11581 ccgggcgcaa cagttacgca accgcgccac ccatgtggaa ctcgccgccg acccgcgctt
    11641 ctccgaactg ttcatgcagc acatgagctt cccggcggga agttgatgat gcgcgcagag
    11701 gcatgagttc gaccccaccg atcgtaacgg cagatccggc aggttcgaat aaccttgcct
    11761 ataaattgac aggattacag gattaaccct aaaaggagca gtatggaccg gcggcggttt
    11821 gcggggaaac cgccccgcca acgggcggca ccgctttccg gtccgtccgc gccagcatcg
    11881 cagggctgac gtattgagat gcgggcgaag cccgcaacct caaaggcggt gagactgtga
    11941 ggctgtggtg aaacagtcga tcccgatagc gatagcgatc ccgaccccga tgaaaccatg
    12001 aaaccagaaa ccatgaaacc agaaacccag aaaccttgaa accatgaaac cttgaaacca
    12061 gaaaccttga aaccaaagaa agtgttctgt ggtgaaacaa tcgatcccga tcccgatagc
    12121 gatcccgacc ccgatgaaac cctgaaacct tgaaaccaga aaccttgaaa cccaagaaag
    12181 tgttctgtgg tgaaacaatc gatcccgatc ccgatagcga
//
LOCUS       DAOSRB010000075        12216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5207_length_12216_cov_5.352027, whole genome shotgun sequence.
ACCESSION   DAOSRB010000075 DAOSRB010000000
VERSION     DAOSRB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12216
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5207_length_12216_cov_5.352027"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLZ74_05625"
     CDS             complement(<1..647)
                     /locus_tag="PLZ74_05625"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOR97836.1"
                     /translation="MKKWMVMFIAVAAMISGCSTVCNGPKNSGTTFAKDALAPFVEKG
                     ELPGAINVFYKDGLQEVACVGYADVAAKRPITMDDVFMQCSQTKGFCGVTIAMLIEEG
                     KITLDDPVSKYLPEFKTLWVHESTTNGVKRLVQAKNPLTIRMVMNHTGGFPFEICAKQ
                     GNIKGGGWSGGAPLRQTAAIAAACPLLFEPGTKVQYSNTGIDIGAAIIEVVTGTR"
     gene            912..1580
                     /locus_tag="PLZ74_05630"
     CDS             912..1580
                     /locus_tag="PLZ74_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97837.1"
                     /translation="MIKSRSCVRSVLVSLVIGFVLAANAALSPTMKIIAPLAGSNLDW
                     GQLHATTVQLNWSWPNGATSAELVIVANGRENAVFSRTFPDTAVRSCSWDMGVPVEDT
                     VYDVTLTFNNNVVQKAQLYANLGSFGGVELKGWNDAGEAKVTKGCVLPYRAAWAEGLA
                     GAATLSIDAEQIALDNSDGYSYLRFSQPGVRQAELDFETEPSSPAFSAYLKVVCNGFV
                     VLFF"
     gene            1595..2944
                     /locus_tag="PLZ74_05635"
     CDS             1595..2944
                     /locus_tag="PLZ74_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97838.1"
                     /translation="MKHLVPVLALSAVSAASAVTLSIDSVKQRWPFSPKIDITFTVGE
                     TGGDFYRLKDFAIYDGNHQVALNGTAAFDNLKPVYSAGTYTLTFDPSKTALTNIVAVQ
                     HFRIGFDISATPTRYLIVNLDKQVDEDGLVEYIDQGDPRLETFVQTVDYLDNGTPTQT
                     TIQYSDAFLSLTNMTVSSGGKTASPYAINKMAFRLVKPGDYRVGKASVSSTYNSVTFT
                     KPYFIAVGLLSRGQYGYIRYGSPWQTDTGTDGNAENQSKSKEIYLDDLRGPHNDPVYP
                     VDWITYGHSVCPTSFIGQARIKYGLAFDQPTEAQWEVAARAGTTGFYYVDVNLTAENT
                     DLLLKLGHEGGTNFNPFRKLANGWGLFDLLGWGQGVLDNAKSSDAGQVNNWVWPSGTD
                     PVGAYTTWNGFCICKGGGHHAGLLEACGSRPTGLSRTKYVNWYDGGYNRVRLVINVD"
     gene            2964..4343
                     /locus_tag="PLZ74_05640"
     CDS             2964..4343
                     /locus_tag="PLZ74_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOR97839.1"
                     /translation="MKKSLFALSLFAGLTTLAATITDITVSPRWPWQDKVDIDYTLDS
                     DVYGGFPVTVALYDGETPLTVAAASLPGNGEVRLPGRRHQVFDFALSGLESRPKALRT
                     VITTSANPVRFMIIDLTKRPGDANQREYIYAGDARLESYVDYPIEYRVSDDEAEGMAV
                     THIDYTDVWFGVTNDNQYARSKMVFRYVPPQAGVKTGDGNVETVFNRVSLTKAYWIAV
                     FPMTQGQYSWLTWDVANRRHRDPNWGMATINETEQQYPMPGGMRDYVGATYTDDPYND
                     YPAGMRGPVGDPVNPVGWPTYGHDVCPTNLLALYRQKTGLRLDYPTEAQWEVACRAGT
                     TGYYYDKESTAQNTNLLQRIANKIDNNVAVGRYLPNTWGLYDMLGYRGEVMLDRYTSD
                     RISGVDPVGDRDPAKSNYTYARVVRGTSSHAEPHAQATQVGSRIADAAAEWFSSLLTT
                     MRWTVELDD"
     gene            4336..5403
                     /locus_tag="PLZ74_05645"
     CDS             4336..5403
                     /locus_tag="PLZ74_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97840.1"
                     /translation="MIDAKTIKKVNTVNVNCKMKHTLSLLSLRRYAIPALLIGLLLFT
                     VSGWLTGLTGRLRWRLDQGNQIYLDASIRDAARLMIPVGTAKAAADAIEGSTVNIEAG
                     AVFAKAGMSIEAGDTLQPLLDYIDLAWRLLMISLVYLVTAKCVLAGTDALSAPFLVLS
                     FSALLAGHLFAMIPGIPPVWRQAFRRFGALFLMAALLFLLILPLTVAGTAYLSRHTTD
                     PMREEVRTAFEQIGSAFSMEQFHAADELKDKALILKEKLASLGEYARVSIKEVAMAVC
                     KLVAIKILNGLVFPLASFAFLVWLVRGCLWPAFGLSDRPLSPEDFRRLATWLDRGKPA
                     THADPPTVLPTEPAESSPGER"
     gene            5887..7173
                     /locus_tag="PLZ74_05650"
     CDS             5887..7173
                     /locus_tag="PLZ74_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOR97841.1"
                     /translation="MNTLALRLYGENDLRLERFPLPEITDDEILADIVTNSICMSDHK
                     AAIQGARHKRVPDDVADHPIILGHEFCGKFLKVGKNWQHKFSVGQKYGVQPQLNIPGH
                     EHRAPGYSFPYTGGNATRIIIPREVLELDCLLIYSGDAYYKSSIAEPVSCIVGAYRCS
                     YHFKAGEYVHQMGIKNGGSMVILAGAGPMGLSAIDVALHGPEHRPGRLVITDIDQARL
                     DRAAQLFPVEHAKTCGVELHYINTAGQDDPVAQIRSVNANQGFDDVMVFAPVPELIEQ
                     GSALLAYLGCLNFFAGPARSDFKAAINFYDVHYMGHHVVGSSGGNTQDLQDAMDYAAQ
                     GLITPSVMITHVGGIDSVAETTMKLPDIPGGKKLVYTHVSMPMTAITDFAARGRTEPF
                     FAALDEICGRNNGLWCSEAENYVLTHAPRLEEDARV"
     gene            7170..7769
                     /gene="ruvA"
                     /locus_tag="PLZ74_05655"
     CDS             7170..7769
                     /gene="ruvA"
                     /locus_tag="PLZ74_05655"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOR97842.1"
                     /translation="MIAFLQGTVVEKTPSSVVLDVHGVGYEVFISLNTYDRLPGSGAP
                     CRLLTHDYVREDARTLFGFMQAEEKAMFERLITVSGIGPKLALSALSGLTVADLSLAI
                     AEGNVKRLSSIHGIGKKTAERIIVELRDKIDPLAALAARSGEARTSEQTIMLRDAILA
                     LGSLGFPQEQARKMVQSALDAEPGIRETEALLRKALSTK"
     gene            complement(7777..8853)
                     /locus_tag="PLZ74_05660"
     CDS             complement(7777..8853)
                     /locus_tag="PLZ74_05660"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOR97843.1"
                     /translation="MAKTYKIAVLPGDGTGPEVIAEGCKVLDAAAQKFGFKLDKAFYD
                     LGGMRYLAIGETLTDATLDELRQFQAIYLGAIGHPDIKPGILEQGVLLKLRFALDQYI
                     NLRPVKLYPGVACPIKDKGPAEIDYCVIRENSGGLYTGIGGLSRPGTRDEVAVQQMIY
                     TYAQVERCLRYAFEYARHNGKKARGKGPENKLALIGKSNVLTHMYALWNRVFDEVGSE
                     YPDVKREYYHVDATCLYMVACPEMFDVLVTENMFGDIITDLAAITQGGLGVAAGGNIN
                     PQGVSMFEPIGGTAPMWTGKNAINPIAAIGAGAMMLTALGETEAGRAIEDAIAKTTPK
                     MKSQMANEMGFTTTQVGDLVAEAL"
     gene            9005..10177
                     /gene="aroC"
                     /locus_tag="PLZ74_05665"
     CDS             9005..10177
                     /gene="aroC"
                     /locus_tag="PLZ74_05665"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOR97844.1"
                     /translation="MALRYLTAGESHGPALTAILEGMPAGLPVTEGQIALDLARRQCG
                     KGSGERMAIEKDAAQILSGVMDGVTTGAPIALLIPNRDHANWKGREIPAYTTPRPGHA
                     DLAAAVKYGYDDIRPALERASARETAMRVAVGALCRALLDNFGITVGGYVSAIGEHTA
                     RLDGLAFAERVSRARASAVQCPDPVADAAMSDAIAKAHDAGETLGGIMEVVADGIPVG
                     LGSHVNADRRLDARLIQALGSVPAIKGVAFGDAFASARQTGTLTNDAIRPDAEGNLLR
                     GSNRCGGIEGGISNGAPIWLRAAMKPIPTTRKGQETVDLATGQTSLTCYERSDVCPVP
                     RAVVVLEAVVAIVLADALIEKLGGDSLSEMRPRFDALRRATLREARMSGNPRLFWP"
     gene            complement(10253..11305)
                     /gene="gluQRS"
                     /locus_tag="PLZ74_05670"
     CDS             complement(10253..11305)
                     /gene="gluQRS"
                     /locus_tag="PLZ74_05670"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HOR97845.1"
                     /translation="MGNDQREVREEALQSDAARGDETGGSAPVRGRLAPSPTGALHLG
                     NARTFLIAWLRVRSRAGALILRMEDLDHPKIKRGAARETLDDLRWLGFDWDEGPDCGG
                     PFAPYTQSERRAYYARVLQRLIARGQVYPCICSRRDVEAGQSAPHAEDGLFYPGTCRG
                     RFADYPDACRVLPTGRLPAWRFRVAAGERVAFDDKFHGRVEQDVAAASGDFVLARDPD
                     GAGYMLAVVADDAAMGITEVVRGDDLLPATPRQILLYRELGFPPPLFVHVPLVVSEEG
                     RRLAKRHGDTRIRAFREAGVPPEKIIGLLAWWCGWAEWGEMLSLNEVLPRFDMRRLPR
                     EPVVLTSAIRRELGIR"
     gene            11516..>12216
                     /locus_tag="PLZ74_05675"
     CDS             11516..>12216
                     /locus_tag="PLZ74_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HOR97846.1"
                     /translation="MSIESLKNYVFTSKYSRYIPEKMRRETFEEAVDRVIAMHRSHFA
                     AKGIELEDLLSFCEQAMKKRMVLGSQRAMQFGGPPILRKNARIYNCTTSYCDRPRFFQ
                     EALWLLLCGCGVGFSVQPHHIDKLPGIRKPAAGHATFIIPDTIEGWADALGVLLSSYF
                     TDAQPFPEYAGQIVQFDYTEIRPKGRPISSGMGRAPGPEPLRRSLEMIRTLVDRRIEE
                     GHGRLRSVDAYDILM"
BASE COUNT         2391 a         3930 c         3523 g         2372 t
ORIGIN      
        1 cagcgtgttc cggtcaccac ctcaataatc gccgcgccga tgtcgatgcc ggtattggaa
       61 tactgaactt tcgtgccggg ctcgaagagc agcggacacg ccgccgcgat cgccgcggtc
      121 tgccgcaacg gcgcgccgcc ggaccagccg ccgcccttga tgttaccctg cttcgcgcaa
      181 atctcgaacg ggaagccgcc ggtgtggttc atgaccatgc ggatcgtgag cgggtttttc
      241 gcctggacaa gccgcttcac gccgtttgtg gtggattcat ggacccagag cgttttgaat
      301 tccggcagat acttcgaaac cgggtcgtca agcgtgattt tcccttcctc aatgagcatg
      361 gcaatggtta cgccgcagaa gccctttgtc tgcgaacact gcataaaaac gtcgtccatc
      421 gtgatcggac gcttcgccgc gacgtcggca taaccgacgc aggcgacctc ttgcaggccg
      481 tccttgtaaa agacgttgat cgcaccggga agctctcctt tttcaacgaa aggcgcgagc
      541 gcgtccttcg caaacgttgt tcccgaattt tttggcccgt tgcagactgt gctgcatcca
      601 gaaatcatcg cggcgacggc aatgaacatc accatccact tcttcatcgt atgtctcctt
      661 ctttccccag tgttgttttt tcgatcggtt gagccgcgcc agacacttga gccggtaccg
      721 gcgtacagac aaagccgaca ttcctttctt ccgtgaatga aaaggtatac tataatatgg
      781 ttttttaaga aagagcaagc gcaacccgtc aaaaaccgga gagcgcgtga agtgcaaaag
      841 acgcgaagcc cattgtccac acggtgaact catgctataa tcgcagtcaa cgcactagga
      901 ggaattcgat gatgatcaaa tcacgttctt gtgttcgctc cgttcttgtc agtcttgtga
      961 tcggtttcgt cttagctgcc aatgccgcgc tttcgccgac gatgaagatc atcgcgccgc
     1021 ttgccggcag taacctcgac tggggacagc tccatgcgac caccgtgcag ctcaactggt
     1081 cctggcccaa tggcgccacg tcggcggagc ttgtgatcgt cgccaacggc agggaaaatg
     1141 ctgtcttcag ccggacgttc ccagataccg ccgtccgttc ctgcagttgg gacatggggg
     1201 ttccggtcga ggacaccgtc tatgacgtga cgttgacgtt caataacaac gtggtgcaga
     1261 aggcgcagct ctacgcgaat ctcggctcgt tcggcggcgt ggaactgaag ggctggaatg
     1321 atgcgggcga ggccaaggtc acgaaaggct gcgtgctacc ctatcgtgcg gcatgggccg
     1381 aaggccttgc gggcgcggcc acgctcagca tcgatgccga gcagatcgcg ctggacaatt
     1441 ccgacggcta ctcctatttg cggttcagtc agcccggagt ccggcaggcg gagctcgact
     1501 tcgagaccga gccttcctca ccggcttttt cggcctatct caaagtcgtg tgtaacgggt
     1561 ttgttgtgct cttcttctag gaaaggatcg tcctatgaag catctggttc ccgttctggc
     1621 gctcagcgcc gtttctgcgg cttccgctgt cacgctgtcc atcgactcgg tcaagcagcg
     1681 ctggccgttc tcccctaaga tcgatatcac ctttaccgtc ggcgagacgg gcggggattt
     1741 ctaccgcctg aaggatttcg ccatatacga cgggaatcat caagtggcgc ttaacgggac
     1801 ggcggccttc gataacctca agccggtcta cagtgcgggc acctacacgc tcacgttcga
     1861 tccttccaag actgccctta ccaacatcgt cgccgtacag cacttccgta tcgggtttga
     1921 catttcggcg acaccgacgc gctacctgat cgtcaatctc gacaaacagg tggacgagga
     1981 cgggcttgtc gagtatatcg accaaggtga tccgcgcctc gagacgttcg tgcagaccgt
     2041 cgactatctg gataacggaa cgccaacgca gacaacgatc cagtattccg acgccttcct
     2101 ttcccttacg aacatgaccg tctcttccgg cggcaagacg gcctcgccat acgcgatcaa
     2161 caagatggcg ttccgtctcg tgaagccggg tgactaccgc gtcggcaaag ccagcgtctc
     2221 gtcgacctac aattccgtca ccttcaccaa accgtacttc atcgccgtcg gtctgctttc
     2281 aagaggccaa tacggttata tccgttacgg cagtccgtgg cagaccgata ccggaacgga
     2341 tggcaatgcg gagaaccagt cgaaatccaa ggagatctat ctggacgatc tgcgcggtcc
     2401 gcacaacgat ccggtctatc cggtggactg gatcacctac ggccactccg tctgcccgac
     2461 gagcttcatc ggtcaggcgc gcatcaaata tggccttgcc tttgaccagc cgacggaagc
     2521 gcaatgggaa gtcgccgccc gcgccggcac gacgggcttc tactacgtcg acgttaatct
     2581 gactgccgag aacactgatc tgctgcttaa gctcggtcac gagggcggca ccaacttcaa
     2641 tcccttcagg aagcttgcca acggttgggg cctcttcgat ctgctcggct ggggacaggg
     2701 cgttctcgat aacgccaaat caagcgatgc aggtcaggtc aacaactggg tctggccgtc
     2761 gggaacggat cccgtgggcg cgtacacgac ctggaacgga ttttgcatct gcaagggcgg
     2821 tggacatcat gccggtctgc tggaggcctg tggatcgcgt ccgaccgggc tttcgagaac
     2881 caagtacgtg aattggtatg atggcggcta caaccgcgtg cgtctcgtca tcaacgtgga
     2941 ctgaagaaag cggagaatct gatatgaaga aatcgctttt cgccctttcc ctgttcgccg
     3001 gacttacgac actggcggcg acgattaccg acatcaccgt ttcgccgcgc tggccgtggc
     3061 aggacaaggt cgatatcgac tatacgctcg attcggacgt ctacggagga tttcccgtaa
     3121 ccgtggcgct ctacgacggc gagacgccat tgacagtcgc ggccgcgtcg ctgccgggca
     3181 acggtgaagt ccgtctgccg gggcgtcggc accaagtctt cgatttcgcc ctgtcgggtc
     3241 ttgagtcgcg cccgaaggcc ctgcgcacgg tcatcacaac gtcagcgaac cccgtccgct
     3301 tcatgatcat cgacttgacg aagcgcccgg gcgacgcgaa ccagcgcgag tacatctatg
     3361 ccggggacgc gcgtcttgag agctacgtcg actatccgat tgaataccgc gtctctgacg
     3421 acgaggcgga agggatggcg gtcacccaca tcgactacac ggacgtgtgg ttcggcgtga
     3481 cgaacgataa ccagtacgcg cgctcgaaga tggtgttccg ctatgtgccg ccgcaggcag
     3541 gcgtcaagac gggcgacggc aacgtcgaga cggtgttcaa ccgggtttca ctcaccaagg
     3601 cctattggat cgcagtcttc ccgatgacac aggggcagta cagttggctc acgtgggatg
     3661 ttgcaaaccg taggcatagg gatccgaact ggggaatggc gacgatcaat gagacggaac
     3721 agcagtatcc gatgccaggc ggtatgcgcg attatgtggg tgcaacgtat acggatgatc
     3781 cgtacaacga ttatccggct ggcatgcgcg ggccggtggg tgatcccgtc aatcccgtcg
     3841 gctggccgac ctacggccat gatgtgtgtc ctacgaacct gctcgcgctc taccgtcaga
     3901 aaaccggcct gcgactcgat tatccgaccg aggcgcagtg ggaagtcgcc tgccgcgccg
     3961 gcaccacagg ctattactac gacaaagagt ctacggcgca gaacaccaac ctcctgcagc
     4021 gtatcgccaa taaaatcgac aacaatgtcg cggtgggaag atatcttccg aacacctggg
     4081 ggctttacga tatgctcggc tatcgcggtg aagtgatgtt ggatcgctat accagcgacc
     4141 gtatttccgg cgtggatccc gtcggcgaca gggatccggc gaagagcaat tatacttatg
     4201 cccgggttgt ccgcggcacg tccagtcatg cagagcctca tgcgcaggcc acgcaagtcg
     4261 gttcgcgcat agcggatgcg gccgcagagt ggttttccag cctcttaacg accatgcgct
     4321 ggacggtcga actggatgat tgacgcgaag acgattaaga aagtgaacac agtgaacgtg
     4381 aactgcaaaa tgaaacacac gctctccctg ctttctttac gccgatacgc aatcccggcg
     4441 ctgttgatcg ggctgttgtt attcacggtt tccggctggc tgaccggcct gaccggtcgg
     4501 ttgcgttggc ggttggatca gggcaaccag atttatctcg acgcctcgat ccgggatgcc
     4561 gcgcgcctga tgattccggt cggcaccgcc aaagcggctg ccgatgcgat tgagggctcg
     4621 accgtcaaca tcgaggccgg agccgtcttt gccaaagcag ggatgagtat cgaagccgga
     4681 gacaccctac agccgttgct cgactacatc gatctcgcat ggcgtctcct gatgatcagc
     4741 ctcgtctacc tggttaccgc caaatgcgtt cttgcgggca cggacgcgct gtccgcgcca
     4801 tttctcgtgt tatcgttcag tgctctgctt gccgggcatc tgttcgccat gatcccggga
     4861 atcccccccg tatggcgtca ggcgttccgt cggttcggcg ccctctttct gatggcggcc
     4921 ctgctgtttc tgctcatcct gccgcttacc gtcgccggaa ccgcctatct gtcacgccac
     4981 acgaccgatc caatgcgcga agaggttcga accgcgttcg aacagatcgg ttccgctttt
     5041 tccatggagc agtttcatgc cgctgacgag ttgaaagaca aagcactgat cctcaaagaa
     5101 aaactcgcct ccctcggaga gtacgcccgt gtgtccatca aggaggtcgc gatggcggtc
     5161 tgcaaactgg tggccatcaa aatactgaac ggactggttt ttccgctcgc ctcgttcgct
     5221 ttcctcgtct ggctggttcg cggatgcctg tggccagcat tcggcctgag cgaccgaccg
     5281 ctctcgccgg aggatttccg acgtctcgca acatggctgg accgtgggaa acccgcgacg
     5341 cacgcggacc cccccaccgt gttgccgacg gaacccgccg agtcctcccc cggggaacgc
     5401 tagagcggtt ttatccatcc cgcgaccgtt cagggaaccc tgcctaccag caccacaccg
     5461 gggcggacag aagcccggtg cgtgcgcctt ggggtgtcat cggcagccag agtttcttcc
     5521 atttcgctcc cggcgtttcc gcgtttccga aaatcggcgc gactgatgtg tggggggtga
     5581 tcctcagttc ggtgctcccc ggtctcacag cgcaaaaacc aggcctgcgg cggaaccgcg
     5641 acacgggccg cccggccaat tgtattcgtc gtaaagccag atcttcctgc cgagccgttt
     5701 ggcgtgtttg cagaaccatt cacagacctg caaccactcc tcgtttcatg aagcttcttg
     5761 tgtttcatgg tgcgcccccc gcccggcata tctttgaact tgccgcgccc cacccggaaa
     5821 gctatgctaa cgtggttttc ggggcgaacc cgacgccatc ataacccgca ccttgaggaa
     5881 aagcgaatga acacccttgc cttgcgcctg tacggggaga acgacctccg cctggagcgt
     5941 ttcccgctgc cggaaatcac agacgacgag atcctcgccg acatcgtgac caacagcatc
     6001 tgcatgtccg accacaaggc ggcgattcag ggcgcccggc acaaacgcgt gccggatgat
     6061 gtggcggacc acccgatcat tctcggccac gagttctgcg gcaagttcct gaaagtcggc
     6121 aagaactggc aacacaaatt ttcggtcggc cagaagtacg gcgtgcagcc ccagcttaac
     6181 atccccggtc acgagcaccg tgcgccagga tattcgttcc cctacaccgg cggaaatgcc
     6241 acgcgcatca tcattccgcg ggaagtgctg gaactggact gcctgctcat ctacagcggc
     6301 gatgcctact acaagtcctc gatcgccgag ccggtctcct gcatcgtggg cgcgtaccgt
     6361 tgcagttacc acttcaaggc cggcgagtac gtccatcaga tgggaattaa aaacggaggc
     6421 tcgatggtga tcctagcggg ggccggcccc atgggactca gcgcgatcga cgtggccctg
     6481 cacggtcccg aacaccggcc cggacgcctg gtcatcacgg atatcgatca ggcccgcctc
     6541 gaccgcgccg cgcagctttt cccggttgaa cacgccaaaa catgcggggt tgaactgcat
     6601 tacatcaaca ccgcggggca ggatgatccg gtcgcgcaga tccgatccgt gaacgcgaat
     6661 caggggtttg acgatgtgat ggttttcgcc cccgtgcccg agttgatcga gcaaggctcg
     6721 gccttgttgg cgtatctcgg atgcctgaac ttcttcgcgg gaccggcgcg ctccgatttc
     6781 aaggcggcca tcaatttcta tgatgtccac tacatgggcc accatgtggt cggttcctcg
     6841 ggcggcaaca cccaggacct gcaggatgcc atggattacg ccgcgcaggg tctgatcacg
     6901 ccgtccgtta tgatcaccca tgtgggcggc atcgactccg tggctgagac gaccatgaag
     6961 ctgcccgata tccccggcgg caaaaaactg gtctacaccc acgtctccat gcccatgacc
     7021 gccatcacgg atttcgccgc gcgcggacgg accgagccgt ttttcgccgc gctggatgaa
     7081 atctgcggac ggaacaacgg gctctggtgt tcggaggccg aaaactacgt gctgacgcac
     7141 gcgccgcgac tggaggagga cgcgcgggtg tgatcgcttt tcttcagggc accgtggtcg
     7201 agaaaacccc ctcgtcggtc gtactcgacg tgcacggcgt ggggtatgag gtgttcatct
     7261 ccctcaacac ctatgaccgg ctccccggta gcggtgcccc ctgccgactg ctgacgcacg
     7321 attatgtccg cgaggacgcc cgcacgcttt tcggattcat gcaggcggaa gagaaggcga
     7381 tgttcgaacg cttgatcacg gtgagcggca tcggcccgaa actggcgctc agcgccctga
     7441 gcggcctgac cgtggccgac ctgagtctcg ccatcgcgga gggcaacgtg aaacggttga
     7501 gttcgatcca cgggatcggc aaaaaaaccg ctgaacgcat catcgtcgaa ctgcgggaca
     7561 aaatcgaccc gctcgcggca ctggccgccc gctcgggcga ggcccgcacc tccgaacaga
     7621 ccatcatgct gcgcgacgcg atcctcgcgc tcggctccct cggcttcccg caggagcagg
     7681 cgcgcaagat ggtgcagagc gccctggatg ccgaacccgg tatccgggaa accgaagcgc
     7741 tgttgcgcaa agcgctcagc accaagtgaa tccagatcag agcgcttcgg cgaccagatc
     7801 acccacctgc gtggtggtga agcccatctc attcgccatc tggctcttca ttttcggcgt
     7861 ggtcttcgca atcgcgtcct caatcgcgcg gccagcctcc gtttcaccca gcgcggtcag
     7921 catcatggcg cccgcgccga tcgcggcaat cggattgatc gcgtttttac cggtccacat
     7981 cggcgcggtc ccgccgatcg gctcgaacat gctgacgccc tgcgggttga tgttgccgcc
     8041 cgccgccacg cccagcccgc cctgcgtgat cgccgccaga tcggtgatga tgtcgccgaa
     8101 catgttttcc gtcaccagca cgtcgaacat ctccggacag gccaccatgt acaggcaggt
     8161 cgcgtccaca tggtaatact cccgcttcac atcgggatac tctgagccga cctcgtcgaa
     8221 cacgcggttc cacagcgcgt acatgtgcgt cagcacattc gacttgccga tcagggccaa
     8281 tttgttctct ggccccttac cacgcgcctt tttgccgtta tgccgagcgt attcgaaggc
     8341 atatcgcagg cagcgctcca cttgggcgta cgtgtagatc atctgctgaa ccgccacctc
     8401 gtcccgggtg cccggacgcg acagcccgcc gatcccggtg tagagcccac cgctgttttc
     8461 gcggatcacg cagtaatcga tctccgccgg gcctttgtcc ttgatcgggc atgcgacacc
     8521 gggatagagt ttcacgggcc tcaggttgat gtactgatcc aacgcaaagc gcagcttcag
     8581 cagcaccccc tgctcaagaa tccccggctt gatgtccgga tgaccgattg cccccagata
     8641 gatcgcctga aactggcgca actcatccag tgtcgcatcc gtcagcgtct cgccgatggc
     8701 cagataacgc atgcccccca aatcataaaa cgccttgtcg agtttgaacc cgaacttctg
     8761 cgccgccgcg tccagcacct tgcacccttc ggcgatcact tccggacctg tcccgtcacc
     8821 gggcagcacc gcgattttgt atgttttggc catatctctc ctttgcgttg aaagaaaaac
     8881 attttaacgg acacgcgccc atgcacacaa ggttttcgtg ccgcaccttg cattccgccc
     8941 ggccttccgg ttaaaatacg ccactggaaa gaaacaccgt atccctaaca aaacggaact
     9001 gtctatggct ctgcgatatc tgactgctgg cgaatcccac ggaccggccc tgaccgcaat
     9061 cctcgaaggc atgccggcgg gtctgccggt caccgaaggg cagatcgccc ttgacctcgc
     9121 gcggcgccag tgcgggaaag gctcgggcga gcgtatggcc atcgaaaagg acgccgccca
     9181 gattctgtcc ggtgtgatgg acggagtcac caccggcgcg ccgatcgcgc tgctgatccc
     9241 gaaccgtgat catgccaact ggaaaggtcg ggagatcccc gcgtacacca cgccgcgccc
     9301 cggtcacgcg gacctcgccg cagccgtcaa atacggttat gacgacatcc ggccagcgct
     9361 ggaacgcgcc tcagcgcgtg aaaccgccat gcgtgtggcg gtcggagccc tctgccgcgc
     9421 cctgctcgac aacttcggga tcacggtcgg cgggtatgtg tccgccatcg gcgagcacac
     9481 cgcacggctg gacggactcg cgttcgctga gcgcgtcagc cgggcccgcg cctctgctgt
     9541 ccagtgcccg gacccggtgg cggacgcggc catgtcggac gcgatcgcca aggcccacga
     9601 cgcgggcgag acgctcgggg gcatcatgga ggtcgtcgcg gacgggatcc cggtcggcct
     9661 cggttcgcac gtgaacgcgg accgccgtct cgatgcccgc ctgattcagg cgctcggcag
     9721 tgtgcctgcc atcaagggcg tggcgtttgg tgacgcgttc gccagcgcgc ggcagacggg
     9781 gaccctcacc aacgacgcga tccggcccga cgcggagggc aatcttctgc gcggttcaaa
     9841 ccggtgcgga ggcatcgagg gcggcatctc caatggggcg ccgatctggt tgcgcgcggc
     9901 gatgaaaccc atcccgacca cccggaaagg gcaggaaacg gtggatctcg cgaccggaca
     9961 aacttcgctt acctgttacg agcggtcgga cgtgtgccct gtgccccgcg ccgtcgtggt
    10021 gctggaggcg gttgtcgcca tcgtgctggc ggacgcgctg atcgagaagc tggggggaga
    10081 cagcctgagc gaaatgcgtc cgcggttcga cgccctgcgc cgggccacgc tccgcgaggc
    10141 ccgcatgtca ggcaaccccc gcctcttctg gccctgacgt ggcggacgga gcccgcacgg
    10201 tagggacgca tccccggggt gtccgcagaa cacggcaggg acctgcggcg gttcagcgga
    10261 tacccaactc ccgccggatc gcagatgtca acacgaccgg ctcgcgcggc agccgacgca
    10321 tgtcgaaccg gggcagcacc tcattcagtg aaagcatctc gccccattcg gcccaaccgc
    10381 accaccaagc cagcagaccg atgatttttt ccggaggcac ccctgcctcg cggaaggcgc
    10441 ggatccgcgt atcgccatgt cgcttggcca gtcggcggcc ctcctcgctc accaccagcg
    10501 gcacatggac gaacaacggc ggcgggaacc ccaactcgcg gtaaagcagg atttggcgcg
    10561 gcgtggcagg cagcagatca tccccccgca ccacctccgt gatgcccatc gccgcgtcat
    10621 ccgccaccac cgccagcatg tagcccgcgc cgtccggatc ccgcgccagc acgaagtcgc
    10681 cgctcgccgc cgcgacatcc tgttcgacac gtccgtgaaa cttgtcgtca aacgccaccc
    10741 tctcccctgc cgcaacgcgg aaccgccagg ccggcaaacg ccccgttggc agcacccggc
    10801 aggcgtccgg ataatccgcg aagcgccctc tgcatgtgcc gggataaaag agcccgtcct
    10861 ccgcatgggg cgcggattgc ccggcctcga catcccgccg cgaacagatg cacggataga
    10921 cctgcccccg cgcaatgaga cgctgcagga cgcgtgcgta atacgccctg cgctcacttt
    10981 gcgtgtacgg cgcgaagggg ccgccgcaat ccggcccttc atcccagtcg aatcccagcc
    11041 atcgcaggtc atccagcgtt tcccttgccg ccccacgttt gattttcggg tggtccagat
    11101 cctccatgcg cagaatcagg gcgcccgccc gactgcgtac ccgcagccag gcaatcagga
    11161 aggtccgcgc atttcccaga tgcaacgcgc cggtcggact cggcgcaagc ctcccgcgaa
    11221 ccggtgcgct cccccccgtc tcgtcacctc gtgcggcgtc ggattgcaga gcctcctccc
    11281 ggacctctct ctgatcgttc cccatgtgtt tttcatgata cccccgcccc caagacggag
    11341 aaagcccaaa aactgcttcc cgatttcttt gagagccaat gagttacgaa aaaccacaaa
    11401 atatggtgga cttcccgggt gacaaccact atatatagag ctccctgatg tcatcagacc
    11461 acaggggccg ggcatcattt cagtcacctt ttcttttctt acgtacaggg aatccatgag
    11521 catcgaatcg ttgaagaatt atgttttcac ctcaaaatac agccggtaca taccggaaaa
    11581 aatgcggcgg gagaccttcg aggaagcggt cgaccgggtg atcgcgatgc accgcagcca
    11641 ttttgcggcc aaggggatcg aactggagga tctgctgagc ttctgtgaac aggcgatgaa
    11701 aaaacgcatg gtgctgggca gtcagcgcgc gatgcaattc ggcggccccc cgatcctgcg
    11761 caagaacgcg cgcatctaca actgcaccac ctcctattgt gaccgccccc gctttttcca
    11821 agaggcgctc tggcttctgt tgtgcggttg cggcgtgggg ttttcggtgc agccccacca
    11881 catcgacaaa ctcccgggca tccgcaaacc cgcagcggga cacgccacct tcatcattcc
    11941 cgacaccatc gagggttggg ccgacgcatt gggtgtactg ctctccagtt atttcacgga
    12001 cgcgcagccc tttccggaat atgcgggaca gatcgtccaa ttcgactaca cggaaatccg
    12061 tcccaaaggc cgccccatct cctctggcat ggggcgcgcg ccggggcccg aaccgctacg
    12121 ccgcagcctg gaaatgatcc gcacactggt cgaccggcgc atcgaagagg ggcacggccg
    12181 tctacgctcg gtcgacgcct atgacatcct catgca
//
LOCUS       DAOSRB010000076        12214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5210_length_12214_cov_8.294103, whole genome shotgun sequence.
ACCESSION   DAOSRB010000076 DAOSRB010000000
VERSION     DAOSRB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5210_length_12214_cov_8.294103"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1706)
                     /locus_tag="PLZ74_05680"
     CDS             complement(393..1706)
                     /locus_tag="PLZ74_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97847.1"
                     /translation="MQNRREFMKGSAVFGAMMAAAPSVVVGQGAARVFKVGMIGAGGR
                     CSGAMANLVEAAKNLGHEVKLVAVCDFFEDRARNAAKKFGCEEKNVFWDANSYKKVVA
                     SDCEIIVTATPPNFRPVHVEAAVKAGKHVFAEKPVAVDGPGVRQFLAAAKLAKEKNLA
                     LVAGTQRRHQKGYLLQALALQQGKIGQVLGGAVYWNGTVPWVRARKEGMRNDAYLCSN
                     WVNWTEMSGDHIVEQHVHNIDVANWFLGRYPRTAGAFGARMRRVSGNQYDFFGVDFDY
                     GDGIHIHSQCRQIGGTSGFVGERFRTEQFEISGGGRIQKVGGDALKLEGTEYTLNGNT
                     YRIKDGNPYVLEHENLLLGLLGQIPIMNEGESVAMSTACAVIGRISAYTGQTVRMSDI
                     IENANSPFYNLACKPTWQEFEAGEVAMPEYGDDQAPLPGKPWGQK"
     gene            complement(1892..2857)
                     /locus_tag="PLZ74_05685"
     CDS             complement(1892..2857)
                     /locus_tag="PLZ74_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOR97848.1"
                     /translation="MKRREFLTTSALVGAGSVCVTSHAAAADACCPASAMPKLTPPKK
                     PAQLNLALQWGAIPGSEIAQKLDFLEANGFAAVEIPSGDWPIKNCDAMIEAMKGRKLF
                     IATACGPSNFAYAEPEKREAEVQKFLPVIEALGKMKSVGLIICPARGKLSEPGWLTAK
                     ELRKDFVENTGKRLAEHAHKHGTSIVLEPLRRNETPFLRQVADGARMAADIGPGATVM
                     GDFWHMGLEETSFMGAFICAGDLLTHVHIAGLKERIIPGVHPDADNYVDGFKGLKLIG
                     YRGAVSFEGGWPKNPADPKKGIPQEEKLRLIRNMVKMLREQWDMA"
     gene            3087..3317
                     /gene="rpmB"
                     /locus_tag="PLZ74_05690"
     CDS             3087..3317
                     /gene="rpmB"
                     /locus_tag="PLZ74_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOR97849.1"
                     /translation="MSRVCEICGKGPMAGNSIIRHGLAKYKGGIGLHTTGISKRRFLP
                     NLHMVRVKSENGTVGRKKVCAACIKQGKIVKP"
     gene            3390..3911
                     /locus_tag="PLZ74_05695"
     CDS             3390..3911
                     /locus_tag="PLZ74_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97850.1"
                     /translation="MQENPADPNVPDANADVLNNPNPENALAQNEEADDEIRATDIVF
                     DCPRCGHNLAIDYRGAGLQIKCVECGEPTLVPIPDGMKIDDLDLSPGELLTQLFQTRR
                     MLLKSEQQISELEETLNSMKLRRTELEKARMNTLRWCAEVVNLCQSALKSQNETALTL
                     NRVIALIGEEQQR"
     gene            complement(4045..4821)
                     /locus_tag="PLZ74_05700"
     CDS             complement(4045..4821)
                     /locus_tag="PLZ74_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOR97851.1"
                     /translation="MKQTDEQLWREARERLLALDTVWPDAPVRRRSLEACRAWLAARH
                     EQPRLSALARLLTPDMTRAQFWCALVPVEREVARIRVTDIEILDRDLTPESEPAAPLM
                     PLTVVTDSLRSAFNVGGIFRTAECFGVAEVVLCGYTPAPDRPQTARAALGAEKLVRWR
                     DGGDVRAALAGLKANGVRCVALETVADAKPLERAEWAFPCALVLGNERFGLDADVIKL
                     CDEVVRIPLCGRKNSLNVVSAFTIAAYTARQWFDMKSKTG"
     gene            4965..6134
                     /locus_tag="PLZ74_05705"
     CDS             4965..6134
                     /locus_tag="PLZ74_05705"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR97852.1"
                     /translation="MKQRMMMAAASLTASAVLAGLYGDTPDARHAWAVHDRNRPNPVK
                     ITAEPGMPPSDATVLFDGSSLEAWESTKGEPTKWRLVDGVLESVKGAGAIRTKQSFGD
                     CQLHLEWAAPTRVEGMGQGRGNSGVFLMSSYEIQILDSYETYTLPDGSNKNPNYADGQ
                     AASVYAENPPLVNACREPGAWQTYDIIFHQPIWEGDKLKYPGSLTVLHNGVLVQDHWE
                     MEGLTTHCRRRPLAPHATKLPLQLQDHGNPVRFRNIWIREIPSRYANTTHGGPAANEA
                     DVMALRKETAAKLFAQILPENPRKAEALHQILEVISYEKAEPYTALAKKLCDAYLNEL
                     AGCGAQQLESRKGEIIGLRNACNVLIRNKIYDERCPLHATLQKIITEQGFEKKKK"
     gene            6199..11790
                     /locus_tag="PLZ74_05710"
     CDS             6199..11790
                     /locus_tag="PLZ74_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR97853.1"
                     /translation="MQRKKGLIACGLVGIALFLGVFILRGFHSRRTTPDAPPRTDAAP
                     AAALPRPAMHRLPQLTTSPAATSATHTTTATTTVGVAACLTEAEIEPLRRAFKPPRFD
                     ADPSLPRERQTLLSNRLLISPVERVLPRAASGAQSPTPRDTTPFLVLFNTPVTEASRA
                     LLIEAGAIPRGFFPNNALLAELTPAILAALAAMPSVQAAEEFVPGDKIQPFLASLLAS
                     HPAETRVRMTVQTLAPEDAESVAAAVTAAGGEVEGFTANTRWGLVQAILPLGAVRTLA
                     TRGEVQWIEERPQPKLFNDFAARGTHLNTTNVWIDWGLTGRGQIAGHADTGLDTAVEE
                     TLHPDFRGRVVALFARGRPGDASDLDGHGTHTAGSLCGDGTASGGQYRGMAWEAGLAH
                     QSVANASGALTGLPYDLYELFEETYACGARVHSDSWGSDTYGTYTSDSRSTDLFAWDH
                     PDHLAVFAAGNAGRDSDRNGVVDPGSVGSPATAKNALAVGATENDRLPGTGGYSAYTW
                     GAAWPNRYPVEPIAGDYLSYSATLNPRYLQGMAAFSSRGPTLDGRVKPDVVAPGTDVL
                     STQSAVGDPVWRAFGANPRYCFGGGTSMSAPLIAGTALLLRQYATERAGITNPSAALI
                     RAMMAGGARSLTPGQYGTTLFREIPAISPNSVEGWGQPDIRDTVHPRRRMVRLYDGIH
                     PGAGQTNLFLIPVSVSHTPLDLVLAWTDYPATAGAGVTRVNDLDLLVTAPNGTVLYPN
                     GGTERDGVNTIERLHLPVAMPGTYIVRVIGTDVPFPGGSAALYVRGGFDDPPLIVHAP
                     VGDRIADGTDLTVRFRVQAVSPFASGEVFMNWASGDATGPTGVWQRAIAEFEGMDGSG
                     FLARIPAQPAGGYVHYTLRVVTKKHDVRLPAAEGSSFSFYVGRPVTLRVSGVPEAYGS
                     VTPPYGTNTVISEIAFVASAPESVTLGKGRRMACSGWTGEGDVPAVGASNRVSLSLSG
                     PSALVWQWRGEVALTNRYRLADVNAIFGETVRWVPEGASAETETALELGFVGNVPYAF
                     CGWRVDGTRWPDADSTSPNPATGIAMYAPRLAEGDYLPFWLDSDDNVLSDWWELRYFG
                     SATSGRDNQDDPDNDGWTNLGEFLDNTDPRDAASQPTPPAITVFALAPLQRDRPPWGV
                     RAEITDNLTVEETLLFWRESGDAAWRQSDMSWIEGNLYGAEIDPPAHGLKTVEYYVAA
                     ADLVGWYDSSFRSTSAVYSVTGAYGEPWMTVTPHAFGLFELSGDATNLAVTVANLAGP
                     DLLWTASVATASAPFAARDEDWVPGGMAGAWCLTTNRTWDGDEVWYCGNPATRRYADS
                     CHAWLDTPVFTVGQGGGALFRQWLRTEWDSGAYFWDGAVVRVSTNLGASFEIITPEGG
                     YPGRIMENPASPFAAHQPCLAGTGGWETVTLDLAAFAGRQVIVRLEFGSDAYVTDEGW
                     YVAGFRVFSLAESAPAWLDPQGTWGGTVPAGSEESFGVRLDSAPLAPGDEVAACLRVT
                     GNDPVGGRVLPVTVRRGHRLSVTAEGPGSVTTDRFFLFRRLGATVILQAHPNAYLDTL
                     TRTGFPVEHFDYRTRVQTVFLGQVETDQTVSGHFTQGLWTLTVVDPYRVGSPANGTYS
                     LTNNTLVEASALTPLLLSDSIRRTCEGWFLSGHTPGTGTTANLTFAITNHATLIWRWM
                     LHYRVQTGVEGGGGRIEPAEAWVPQYERIELTALPDTGYRFDNWLAVPGNATVTSNRI
                     HFTVLAPQFVRASFLAETTRTGVPHLWLAAFGLGNDWEAAALEDVDGDGMPAWAEWLA
                     DTDPTDARSYLALTGLALENDLGRIRWIGGHARTQVLEFATSPAGPWRPVATNLPPTP
                     ITNTVMLPITGDAGFYRLRIP"
     gene            complement(11792..11865)
                     /locus_tag="PLZ74_05715"
     tRNA            complement(11792..11865)
                     /locus_tag="PLZ74_05715"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11831..11833),aa:Gln,seq:ctg)
BASE COUNT         2248 a         3916 c         3767 g         2283 t
ORIGIN      
        1 gacgcccgcg tttccgacac cgcaggtcgc tcgtcgcggg tcgacatata gggtctgacg
       61 ggagaagcgg gcgttccgcc cgctggcagg gacggttccc cgaaccgtcc gcggacgcct
      121 cggggaggca tccctaccgg ttggcggacg ccttcgtccc gcatacgcac cggtctccaa
      181 aactcagtcg attgcgcttg cgacgaagtg gagcgatgaa gtgccgggac gaagagttca
      241 aaaagagcgc cggacgcacc gttgcgtttt gcgccaaaca ggtgcggcgg gggccgcgac
      301 aaaacccaat gtttgcgaca ggccatttgg ggggtaaaaa caaacgcccg tccggcaaac
      361 cggacgggcg ttgcgataca gcggggcacc cgttacttct gtccccaggg cttgcccggc
      421 agcggtgcct gatcgtcacc gtattccggc atcgccacct cgccggcctc aaactcctgc
      481 caagtcggct tgcaggcgag gttgtagaaa ggcgagttcg cattttcgat gatgtccgac
      541 atgcggaccg tctgtcccgt ataggccgag atgcggccga tgaccgcgca ggccgtggac
      601 atcgccacgc tctcgccttc gttcatgatc ggaatctgac cgaggaggcc gagcagcaga
      661 ttctcgtgtt ccagcacata ggggttaccg tctttgatcc ggtacgtgtt gccgttgagc
      721 gtatactccg tgccctccag cttcaacgca tccccgccga ccttctggat cctgccgccg
      781 ccggagattt cgaattgctc cgtgcggaaa cgctcaccca caaagccgct ggtcccgccg
      841 atctgacggc actggctgtg gatatggatc ccgtcgccgt aatcgaaatc cactccgaaa
      901 aagtcatatt gattacccga cacgcgacgc atgcgtgcgc cgaacgcgcc cgccgtgcgc
      961 ggataacggc cgaggaacca gttcgcgacg tcgatgttgt gaacatgctg ctcaacgata
     1021 tggtcaccgg acatctcggt ccagttcacc cagttgctgc agaggtaggc atcgttacgc
     1081 atcccctctt tccgcgcgcg cacccaaggc accgtcccgt tccaatagac cgcgccgccc
     1141 agcacctgcc cgatcttccc ttgctgcagc gccagcgcct gcaacagata tcccttttgg
     1201 tggcggcgct gcgtgccggc caccagtgcg agattcttct ccttggccag cttggccgcc
     1261 gccagaaact ggcgcacacc cggcccgtcc acggccaccg gcttctccgc gaacacgtgt
     1321 ttgcctgctt tgaccgccgc ttcaacgtga accggccgga aattgggcgg tgtggccgtc
     1381 acgatgattt cacagtcgga cgccacaacc ttcttgtagc tgttcgcgtc ccagaacaca
     1441 ttcttctcct cgcaaccgaa cttcttggcc gcattccggg cgcgatcctc gaagaaatcg
     1501 cacacggcca ccagtttgac ttcatggcca aggtttttag ccgcctccac cagattcgcc
     1561 atcgcgccgg aacagcgtcc gcctgcgccg atcatgccaa ccttaaacac ccgtgcggct
     1621 ccctgaccga ccacgaccga cggcgccgcc gccatcatcg ctccgaagac cgccgaccct
     1681 ttcatgaact ctctacgatt ttgcatctct ctctctccta ctaactcact tgcccataaa
     1741 ttgacaggat tacaggattt tcaggattaa aaaatcatgt taatcatgta atcttgtcat
     1801 aaaaaactta tcccagagtt ttctcgcggc tccggcgcgc ggcgggctaa ctcactaact
     1861 cactaatgaa ctaatgaact aatgaacttc ttcaagccat gtcccattgc tcgcgcagca
     1921 tcttgaccat gttacgaatc agccggagct tctcctcctg cgggataccc tttttcggat
     1981 ccgccggatt cttcggccac ccgccctcga aactcaccgc accgcggtat ccgatcagct
     2041 tgaggccctt gaagccatcc acatagttat cggcgtcggg atgcactccc gggatgatcc
     2101 gttccttcag ccccgcgata tgcacgtggg tcagcaggtc gccagcgcag ataaaggcgc
     2161 ccatgaaact cgtctcctcc agtcccatgt gccagaaatc acccatcacc gtcgcgcccg
     2221 gcccgatgtc cgcagccata cgcgcgccat ccgccacctg ccgcaggaac ggcgtctcgt
     2281 tgcggcgcaa cggctccagc acgatcgacg tgccatgctt gtgcgcatgt tccgccagac
     2341 gcttgccggt gttctcgaca aaatccttgc gcaactcctt ggcggtcaac catcccggct
     2401 cgctcaactt gccgcgcgcc gggcagatga tcaagcccac cgacttcatt ttgccgagcg
     2461 cctcgatgac cggcagaaac ttctgcactt cggcttcacg cttctccggt tccgcatacg
     2521 caaaattgct cgggccgcac gccgtggcga tgaacagctt gcgccctttc atcgcttcaa
     2581 tcatcgcgtc acagttcttg atcggccagt cgcccgacgg aatctcgacc gcggcaaagc
     2641 cgttggcctc cagaaaatcc agcttctgcg cgatctcgga tcccggaatc gcgccccatt
     2701 gcagagccag gttaagttgc gcgggttttt tgggcggtgt cagtttcggc attgcgctcg
     2761 cgggacaaca ggcgtccgcg gcagcggcat gcgacgtcac acatacggaa cccgctccga
     2821 ccagcgccga agtcgtcaag aattcgcgtc gtttcatacg tttctctccc ctgattcccg
     2881 gttcgcaccc tcttgcccgg gaaacatgtt gatacaatta gggtattggg tatagaatta
     2941 tcatggacgg ctgttccgcg tcaagaagcc gattacataa agtcgtaatc aacatgaaaa
     3001 aggtcgatac gaaaatcacc tttctgcttg tgggaacgat tccgaaatgc tatgatcaac
     3061 cccctttttg ttaaggagaa acggtcatgt ctagagtatg tgagatttgc ggcaagggac
     3121 cgatggcggg gaattcgatc attcggcacg gtttggccaa atacaaaggc gggatcggtc
     3181 tgcacacgac cggtatctcc aagcgccggt ttctgccgaa cctccacatg gtccgcgtca
     3241 aatccgaaaa cggtaccgtg ggacgaaaaa aggtttgcgc cgcctgcatc aagcagggta
     3301 agattgtcaa accttgaggc catgtgccgc gcgtaggggt tgcggcgagg gtttcgtgaa
     3361 cacaacggaa ttatccgaaa gggggcgtta tgcaagagaa tcctgcggat ccgaacgtgc
     3421 ctgatgcgaa cgcggatgtg ctgaataacc cgaatccgga aaacgcgctt gcccaaaacg
     3481 aggaggcgga cgacgagatc cgtgcaacgg atatcgtgtt cgactgcccc cgttgcggac
     3541 acaacctcgc catcgattac cgtggcgcgg gcttgcagat caagtgcgtg gagtgcggcg
     3601 aacccacact cgtcccgatc ccggacggaa tgaagatcga tgatctcgac ctttcgcccg
     3661 gggagttgct cacgcagctc ttccagactc ggcgtatgct gctcaaaagt gagcagcaaa
     3721 tttctgagtt ggaggagacg ctcaacagca tgaagctgcg ccgtacggag ttggaaaaag
     3781 cccgtatgaa cacgctccgc tggtgcgcgg aagtggtcaa tctctgccag agtgcgctga
     3841 agtcccaaaa cgagaccgct ctcaccctga accgggtgat cgctctgatc ggcgaagagc
     3901 aacaacgctg aggcgtcacg agcccctgat tgtcggcagc agtagcccgg ccgggggtgc
     3961 gctcaccctc tgcgacttgc gacggggatg ctcccgattc gactgatccg ccgttgcggg
     4021 cggggccctc cgcattcccg gctttcagcc cgtctttgat ttcatgtcga accattggcg
     4081 cgcggtatac gccgcgatgg tgaaggcact gacgacattc agggagtttt tgcggccgca
     4141 caacgggatg cgcaccacct catcgcacag cttgatcaca tcggcgtcca acccgaaacg
     4201 ctcattgccc agcaccagcg cgcacgggaa ggcccattcc gcccgttcca gaggtttcgc
     4261 gtccgccacg gtctccaacg ccacgcaccg cactccgttg gctttcaatc ccgccaacgc
     4321 cgcgcgcacg tccccgccgt cacgccagcg caccagtttc tcggctccga gtgcggcacg
     4381 cgccgtctgc ggccggtccg gcgcgggggt gtacccgcag agcacgacct ccgcaacccc
     4441 gaaacactcg gccgtgcgga agatcccgcc gacattaaag gccgagcgca acgaatccgt
     4501 gaccacggtc agcggcatca gcggcgcggc gggttcggac tccggagtga ggtcacggtc
     4561 cagaatctcg atgtcggtga cgcggatgcg ggcaacttca cgttccaccg gcacgagcgc
     4621 gcaccagaac tgcgcccgtg tcatgtcggg cgtaagcagc cgggccagcg cactcagacg
     4681 gggttgctcg tgccgtgcgg ccagccacgc gcggcaggcc tccagactgc ggcggcgcac
     4741 cggagcgtcc ggccacaccg tatccagcgc cagcagtcgc tcgcgcgcct cacgccataa
     4801 ctgttcgtcc gtttgtttca ccgtaacagg cctccgcgcc ccgcccgcag gcggaaacaa
     4861 gacagtatga cacaacccgg acgtttttcg ctatggtaaa gccgccccgt ttttggcaca
     4921 ctcatttgaa aacaagccga attatggcag aactcggagg aaagatgaaa cagcggatga
     4981 tgatggcggc ggcaagcttg acggcctccg ccgtgttggc ggggttgtat ggggacacac
     5041 cggacgcgcg gcacgcgtgg gctgtgcatg atcgaaaccg gcccaatccg gtcaagatca
     5101 cggcggagcc ggggatgccg ccctcggatg cgactgtgct gtttgacggc tcttcgctgg
     5161 aagcgtggga gagcaccaag ggcgagccca cgaagtggcg cctggtggac ggagtcttgg
     5221 agtcagtgaa aggcgccggg gccattcgca ccaaacagag cttcggtgac tgccaactgc
     5281 atctggagtg ggccgcgccg actcgtgtcg agggcatggg tcagggccgt ggcaacagcg
     5341 gggtctttct catgagtagc tatgagattc agattctgga ctcgtacgag acgtacacgc
     5401 taccggacgg cagcaacaag aatcccaact atgccgacgg gcaggcggcc tcggtctatg
     5461 ccgagaaccc gccgttggtg aacgcctgcc gcgaaccggg cgcgtggcag acctatgaca
     5521 tcattttcca ccagccgatc tgggagggcg acaaactgaa gtatcccggt tcgctgaccg
     5581 tgttgcacaa cggtgtgttg gtgcaggacc attgggagat ggagggcctg accacgcact
     5641 gccggcgccg gccgctcgcg ccgcacgcga cgaaactgcc gttgcagttg caggatcacg
     5701 gcaaccccgt gcgcttccgt aacatctgga tccgcgagat cccgtcgcgt tacgccaaca
     5761 ccacccacgg cggcccggcc gcaaatgagg cggacgtgat ggctctgcgc aaagagacgg
     5821 ctgccaagct cttcgcccag attcttccgg agaacccccg caaggcagag gccctgcacc
     5881 agattctgga ggtgatctcc tacgagaagg ctgaaccgta caccgcactc gccaagaagc
     5941 tgtgcgacgc ctatctgaac gaactggcgg gttgcggggc gcaacaactg gaaagccgca
     6001 aaggcgagat catcggtctg cgcaacgctt gcaatgtctt gatccgcaac aagatatacg
     6061 acgagagatg cccgttacac gccacgctcc agaagatcat cactgaacaa gggttcgaaa
     6121 aaaagaagaa gtgagaagag aagcaagaag gaatacgtac cctatacccc ctttccgtct
     6181 tgtgttatgc ttccggtcat gcaacgcaaa aagggtttga ttgcgtgcgg cttggttggg
     6241 atcgcgctgt ttctgggggt ttttatcctg cgggggttcc acagccgccg tacgacgcct
     6301 gacgcgccgc cccggacgga tgccgcgcct gccgcggcat tgccgcgtcc agcgatgcac
     6361 aggctgccgc agttgacgac atccccggcg gcgacctcag cgacgcacac caccaccgcc
     6421 accactaccg tcggcgtggc ggcgtgtctg acggaagcgg agatcgaacc gctgcgccgg
     6481 gccttcaagc cgccgcgttt cgatgcggac ccgtccctgc cgcgtgaacg ccagacgctg
     6541 ctctccaacc gtctgctgat cagcccggtt gaacgggtcc tgccccgggc cgcctcgggt
     6601 gcgcagtctc ccaccccgcg cgacaccacg ccctttctgg tgcttttcaa cacgccggta
     6661 accgaagcct cacgcgcgct cttgatcgag gcaggggcga tcccgcgcgg ctttttcccg
     6721 aacaacgcgc tgttggctga gctgacccct gctatactgg ccgcgcttgc ggccatgccc
     6781 agcgtgcagg cggccgagga gttcgtgccc ggtgacaaaa tccaaccctt tctcgcctcg
     6841 ctcctcgctt cgcatccagc agagacccgg gtacggatga cggtgcagac cttggcgcct
     6901 gaggatgccg agagcgtggc ggctgccgtg actgcggcgg gcggtgaggt cgaggggttc
     6961 accgccaaca cccgctgggg cctggtgcag gcgatcctgc cgcttggcgc ggtgcggacg
     7021 ctggcgaccc ggggcgaggt gcagtggatc gaggagcggc cgcaacccaa gttgttcaac
     7081 gacttcgccg cgcgtggtac gcacctcaac accacgaacg tttggatcga ctggggtctg
     7141 actggccggg gccagatcgc ggggcacgcg gacaccggac tcgacacggc tgtggaggag
     7201 acgctgcatc cggatttccg cggccgcgtg gtggcgcttt tcgcccgcgg ccgaccgggt
     7261 gatgcctcgg acttggacgg acacggcaca cacaccgcag gctcgctgtg cggggacggt
     7321 acggctagcg gcggacaata ccgtggcatg gcgtgggagg cggggctcgc gcatcagtcg
     7381 gtggccaacg cttcgggtgc gctcacaggt ctgccgtacg acctgtacga acttttcgag
     7441 gaaacctatg cctgcggtgc acgcgtgcac tccgacagtt ggggttcgga cacctacggc
     7501 acctacacct ccgactcgcg ctctaccgac ctctttgcgt gggatcaccc tgaccatctc
     7561 gcggtcttcg cggcgggtaa cgccggtcgt gacagtgatc gcaacggtgt ggtggacccg
     7621 ggttcggtgg gctcgcctgc cacggccaag aacgcgctgg ccgtgggtgc cacggagaac
     7681 gaccgcttgc cgggcaccgg cgggtattcg gcctacactt ggggcgcggc ctggccaaac
     7741 cgctacccgg tcgagccgat cgccggagat tacctctcct acagcgccac gctgaatccg
     7801 cgttatctgc agggtatggc cgccttctcc agccgcggac ccacgctcga tggccgtgtc
     7861 aaacccgacg tggttgcgcc cggtacggat gtgctctcca cccaatccgc ggtcggcgat
     7921 ccggtctggc gcgccttcgg cgcaaacccg cgttactgtt tcggcggcgg caccagcatg
     7981 tccgccccgc tgattgcggg cacggctctg ttgctgcggc agtacgccac ggaacgcgcc
     8041 gggatcacca acccctcggc cgcgctgatc cgcgccatga tggccggcgg cgcgcgttcg
     8101 ctcacgccgg gccagtacgg caccacgctc ttccgcgaaa tcccggcgat ttcgcccaat
     8161 tcggtggagg gatggggcca gccggatatc agggatacgg ttcacccgcg gcgtcgcatg
     8221 gtgcggctct acgacggcat ccaccccggt gcggggcaga ccaacctttt tctcatcccg
     8281 gtcagcgtga gccacacgcc gctggatctg gtgctggcgt ggaccgatta tccggccacg
     8341 gcgggcgcgg gcgtgacgcg cgtcaatgat ctcgatctgc tggtgaccgc gcccaacgga
     8401 accgtgcttt atcctaacgg tggcacggag cgggacgggg tcaacacgat cgaacggctg
     8461 cacctcccgg tcgcgatgcc gggaacctat attgtgcggg tgatcgggac cgacgtgcct
     8521 ttccccggcg gcagcgcagc gctttacgtg cgcggggggt tcgacgatcc gccgctgatc
     8581 gtccatgccc cggtcggaga ccgtatcgcg gacggcacgg atctcacggt gcggttccgg
     8641 gtgcaggccg tgtctccctt cgcgtccggc gaggtcttca tgaactgggc atccggagac
     8701 gctacgggcc cgaccggtgt ctggcagcgg gcaatcgcgg agttcgaggg catggacggg
     8761 agtggatttc tggcgcggat ccccgcacag cccgctgggg gttacgtgca ttacacgctg
     8821 cgggtggtca ccaaaaaaca cgatgtgcgg ctccctgctg cggagggttc atccttctcc
     8881 ttctatgttg gccgaccggt gaccctgcgg gtgtcgggcg taccggaggc gtacggcagt
     8941 gtgacgccgc cctacggtac caacaccgtc atctccgaga tcgcgtttgt ggcctccgca
     9001 cccgaaagcg tgacgctcgg caaggggcgg cgtatggcct gttccggctg gaccggcgag
     9061 ggcgatgtgc cggcggtggg cgcctccaac cgcgtgagtc tgagcctgag cggaccgagc
     9121 gccttagtct ggcagtggcg cggcgaggtc gcgctcacca accgctaccg actcgccgac
     9181 gtgaacgcga tattcggcga gaccgtccgc tgggttccag aaggcgcgtc ggccgagacc
     9241 gagaccgcgc tggaactcgg cttcgtgggt aacgtgccct acgctttctg cgggtggcgc
     9301 gtagatggca cgcgctggcc ggacgcggat tcgacgtcgc ccaaccctgc gaccggtatc
     9361 gcgatgtatg ccccgcggct ggccgagggg gattacctgc ctttctggtt ggatagtgac
     9421 gacaacgtgt tgagcgactg gtgggagctc cgctatttcg gcagtgcgac aagcggccgg
     9481 gataaccagg atgatccgga caacgacggg tggaccaacc tcggcgagtt tctcgacaac
     9541 acggacccgc gcgatgcggc gagccagccg actccgcctg cgatcacggt tttcgcactc
     9601 gcgccgctgc agcgcgaccg tccgccatgg ggcgtgcggg ccgagatcac cgacaatctg
     9661 accgtggagg agaccctgct cttctggcgc gagtccgggg acgcggcatg gcggcagagc
     9721 gacatgtcct ggatcgaagg gaatctgtac ggggcggaga tcgacccgcc cgcgcacggg
     9781 ctgaagacgg ttgaatatta tgtggcggcc gcggatctgg ttggctggta cgattcctcg
     9841 ttccgttcca ccagcgcggt gtattcggtc accggcgcct acggggagcc ctggatgacg
     9901 gtcacgccgc atgccttcgg tctgttcgaa ttgagcggcg atgccacgaa tctcgcggtc
     9961 acggtagcca atctcgcggg accggatctg ctctggaccg ccagcgtggc tacagcttcc
    10021 gcgccgttcg ccgcgcggga cgaagactgg gttccgggcg gcatggccgg agcgtggtgt
    10081 ctcaccacca accggacgtg ggacggcgac gaagtgtggt attgcggcaa tcccgcaacg
    10141 cgtcgatatg ccgacagttg ccatgcctgg cttgacacgc ccgttttcac ggttggtcag
    10201 ggcggcggtg cgctcttccg ccagtggctc cggaccgaat gggatagcgg tgcctatttt
    10261 tgggacggcg ccgtggtccg cgtctccacg aatctggggg cgagctttga gatcatcacg
    10321 ccggagggcg ggtatcccgg ccggattatg gagaaccctg cttccccctt tgcggcgcac
    10381 cagccttgtc tggcgggtac gggtggctgg gagacggtga ccttggatct ggccgccttc
    10441 gcgggacgcc aagtcatcgt gcgcctcgag ttcggttccg acgcctacgt cacagacgag
    10501 gggtggtatg tggccgggtt ccgggtcttc tcccttgccg aatccgcgcc cgcatggctc
    10561 gatccgcagg gcacttgggg gggtacggtt ccggcgggca gcgaagaaag cttcggcgtg
    10621 cggctcgatt ctgcgccgct cgcgccggga gacgaagtgg cggcatgttt gcgcgtaacc
    10681 ggaaacgatc cggtcggtgg ccgggtgctc cccgtcactg tgcggcgcgg ccaccggctt
    10741 tcggtgacag ccgaggggcc gggcagcgtt acgaccgatc ggtttttcct cttccgcagg
    10801 ctgggcgcaa ccgtcatatt gcaggcgcac cccaacgcct acctcgacac cctgacacgg
    10861 accggcttcc cggtggaaca tttcgactac cggacgcgcg tccagaccgt gtttctgggg
    10921 caggttgaaa cggaccagac ggtcagcggc catttcacgc agggcctatg gaccctgacg
    10981 gtggtcgacc cctaccgtgt cggttctccg gcgaacggga cctacagcct gaccaacaac
    11041 acgctcgtcg aggcctcggc cctcacgccg ctgttgcttt ccgattcgat tcgccggact
    11101 tgcgagggtt ggttcctctc aggacatacg cccggcaccg gtacaacggc gaacctcacc
    11161 tttgccatca ccaatcacgc cacgctgatc tggcggtgga tgctgcatta ccgcgtccag
    11221 accggagtcg agggcggcgg cgggcggatc gaaccggcgg aggcctgggt cccgcagtac
    11281 gaacggatcg aactgaccgc ccttccggac acgggatatc gtttcgataa ctggctggcc
    11341 gtgccgggca atgctacggt cacaagcaac cgcatccatt tcacggtgtt ggcgccacag
    11401 ttcgtccgag cctccttcct tgccgagacg actcgcacgg gtgtacccca tctgtggctg
    11461 gccgcgtttg gtttgggcaa cgattgggag gccgccgcct tggaggatgt ggacggcgac
    11521 ggcatgcccg cttgggccga gtggctggcg gacaccgatc cgaccgacgc ccgctcatat
    11581 ctggcgctta cgggactcgc gctcgaaaac gacttgggcc ggatccgttg gatcggcggt
    11641 cacgcgcgta cccaagtgct cgagttcgcg acgagtccgg ctggcccctg gcgtccggtc
    11701 gccaccaacc ttccccctac cccgatcacc aacaccgtga tgctgcccat aaccggagac
    11761 gcgggtttct accgtctccg cattccgtaa gtggttgcct cattaggatt cgaacctaaa
    11821 caaacagatc cagagtctgc tgtgctaccg ttacaccatg aggcaaaacg ccttttcatt
    11881 gcaaaaagga gtaaataaga tagccaaagg catggcataa aggcaatcct gtttttcgct
    11941 ttgccgtaat cggtttgtga caggggcgtg cgaagatcat gcgtttccgg tgaaaagggg
    12001 agcagagagt cgggagcgcg ggcgtccccg cccgcaacaa cgtgtcttgc gttttctggt
    12061 tttatccaca gacttgcaca gattgcgcaa atttttcttt ttgtctttga ccgtaaggag
    12121 gggagggacg agcgtctgct cgtccgggcg gcgtttcacg ccgcctttga acgcccaacg
    12181 cccaacgccg aactcagaac gtcgaagtga aaaa
//
LOCUS       DAOSRB010000077        12178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5221_length_12178_cov_5.990184, whole genome shotgun sequence.
ACCESSION   DAOSRB010000077 DAOSRB010000000
VERSION     DAOSRB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5221_length_12178_cov_5.990184"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PLZ74_05720"
     CDS             complement(<1..1332)
                     /locus_tag="PLZ74_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOR97854.1"
                     /translation="MRKGFTLLETIFAVAVFSLLMGMTLGSWLLFMYKSNRVNTQASL
                     DMDVRKVIERFRYELRNTARETVIFYPENQEPYQAVGFALPGSGTDGLVEMAAGGTNI
                     LWRESVVYHVWNHSPHQMRRTVFANRNPNALYADRYGQIAAVVAAGNGASACLAGESA
                     STTVMFENLFTGRLWHASAQFDGYAPTPNTRDRITFGSLSLGPGEHTVSFTVKGKNKA
                     ATSQALRLDRILASVSGWPQEAEWRTTSGAATAPYHVGQNKAGAAYGLAAAGVEVGDT
                     LHVTVRNDAIEECEFIGEGRNVTFSNTVVRFDTIDPPSGFAEGTYAVRIDGQFGAEKN
                     WSCAEQTGHYRNDYFKPLTNCVIRIPVMADPTRSATSGEPVLYGIKKDGYGPVFRLYK
                     SLYNGGLQVLNPSFAVVALKDVPTDYNSNLLPKLDPEERIPLEFWQDGAKKT"
     gene            complement(1347..1823)
                     /locus_tag="PLZ74_05725"
     CDS             complement(1347..1823)
                     /locus_tag="PLZ74_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOR97855.1"
                     /translation="MGRTDQSGMTVVELMVAMGLLVIILTAVFPLVDQMISRFQMARD
                     HYVAASLSQGRIERARGVPYSDLHLYRELGTRVDDFGNAADPAGRFLRKTEVTVNSPT
                     TNMTHMTVTTYPCTCSRWGWRKVFHPIKTGKFICRFDELDFERMDYLFTEELKRTP"
     gene            complement(1837..2976)
                     /locus_tag="PLZ74_05730"
     CDS             complement(1837..2976)
                     /locus_tag="PLZ74_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOR97856.1"
                     /translation="MSTNDKSQTIKIDIRSLGYGRSTVADRSGVEVQSDYFGKLDYRF
                     LFESAYDAILLADRHGIVLEANARATSFFQYGERERGLTGMPVETLVAGMTSSLLEEI
                     LAAITESRYMRIQAFAVRRDGSLTAVEIVAMGNGLRVPEPVCYLIRDIQSRWQAEQRL
                     LSVYHAMDNTDSGIGITDMEGKVVYANRMMVHLLAGGDETEVFGVNLSTWFHRKAVIM
                     PMLENIRQRKSWIGEQRLLVNEQAHWLQIAAVPDINDDNELRGMVVSLRDTAERRRAE
                     IAESQVERNRVMMESLAGVCHSLGQPATVLLTSIEMLKMGGVKDEKARQEIINLCYDA
                     VIQLRDLLQKMNAKRAYAVEPYLPGQEDTKDMLRIEEDQVPSRQT"
     gene            3322..4338
                     /locus_tag="PLZ74_05735"
     CDS             3322..4338
                     /locus_tag="PLZ74_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97857.1"
                     /translation="MATGLSISALTRQSKPLTDIVGIEVGSGNPDGVPAVRLRKRNGR
                     FELLAAGFLPLPGTLPLDLETAQNSTPVWLLPRPFQAPYAALALSSPLGFLRHAAETG
                     EETPDKHATPFRTASRASTPESPALLTGLPEFQAAWAARLLPEGRRPTARSIQFSPAA
                     ALNALTTSPGFKTVSGTAIALFVFAEFTSITAFQEGQLMLYREHAAGYLHLRKVVSQH
                     MGIDMALADSLLDDTLVDPTPIIEPVLRPLFRQVEISSDFLLRRRNCKADHFFLCGVP
                     AGGRYWSSVFAQMMSHTLTPLPPLDGAEALPRSNFGAESLSPIRKQLLMTAYGAARAA
                     LEDA"
     gene            4335..4982
                     /locus_tag="PLZ74_05740"
     CDS             4335..4982
                     /locus_tag="PLZ74_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97858.1"
                     /translation="MSNVYHLNLLQPEEHLSSSPIRLRVMLPMFALLSALGVGVWWSV
                     LAGRVHAVTQQKSAIEANLAQLKPTHETVLGLRTEQHDLTATLEQLAYYTRAQLRFGP
                     SLQALAHHVPDQIQLTELHIPPPPEPPPPDPKNPVWGPTNPIERVTLRLTGRTTGASQ
                     VETLLRAFRTEAFTNLIRSAQIPKGAYRQDTTRRGAAKETLLFELNCECALRRFE"
     gene            4979..5590
                     /locus_tag="PLZ74_05745"
     CDS             4979..5590
                     /locus_tag="PLZ74_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97859.1"
                     /translation="MSLNTSIIHKIAPSMQRSILITLGGLMIAGAMVFFLVLPARARL
                     QTLAAELNTLRDTLATVRADIAGTEQQRLKTAEVRSKHDALVRSGVLQPLLNSFAMRG
                     KSLLDPLAQQTGFAIANVKEDRFLRLQVPKQVPLHLYGRQMIEFSGQGAYTSIVNFIA
                     LAEERFPLCTLAGLKIISQNQTPETHKAFITFEWPAKGEKRTP"
     gene            5689..6141
                     /locus_tag="PLZ74_05750"
     CDS             5689..6141
                     /locus_tag="PLZ74_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97860.1"
                     /translation="MSVLALFLLAALPARAARDPFWPIGYEPPKPEPVVKEIPEVPKK
                     TVPKPVPPKPKPPAVKPVTDGDWTDARAFITVTGFTRATRPDTGETRTLALINRRSYA
                     AGDTLCITNAGIVFLWRIDSLDNRELKLAPIQATRIVPPVSGPSGSAP"
     gene            6202..7986
                     /locus_tag="PLZ74_05755"
     CDS             6202..7986
                     /locus_tag="PLZ74_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOR97861.1"
                     /translation="MNRITRLCAFIATGLCLAWQPVTCAQEAAAQTPQPPKTTASVRV
                     TDASSPSEPLVSMTFDEAPLADVIKAFRDATGANIISGGTNVLQANISVSLDNVPWRK
                     GLSSILDPQGLQLVEQPTGSGIYVINTKTVEVPKITRVFSLEYIKAEDVADLLKTTLG
                     TTGGIVTPFKESNAVILTATEQQISECESIIKAIDKASPQVYIEVRFVELTAQASRKL
                     GLKWDSLGTKEGWGAHADPTFFNFENDYLKTKDKGMDRSQNSERVQPGDTTWRDSLTR
                     TLNRNDSSTQTRTFTGTLSLDAFSLTMNAFEQMDGVSIFSNPKIIVANEKEALVDMTT
                     KEPNLTVTSSRTGTAGDQLDISAELEVIPGEKGDKDGKGKGIFAGEAFFSYGITLKVT
                     PRISSTGLITLNVEPTISDRKSYYEFSGIEETKTPTPKYPIIYMQRLQTVFTMQDGST
                     AVIGGLTTTTEDNIDSGIPWLRELPWVGPRLFGWKSRRKEQKEIIIFVSVGLADPLKL
                     PESVGMPKNAILGRDLLTGELKEPGDRTKEEVMSLRDPVHGTKAQPSTEEVAVPAAVA
                     PDVVVNEKEAAKPVTQQQPVDTAPLLQN"
     gene            8127..9602
                     /locus_tag="PLZ74_05760"
     CDS             8127..9602
                     /locus_tag="PLZ74_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOR97862.1"
                     /translation="MATLDRFLIPLVTKSGSDLHLSAGRPPVMRIHGTLAPVPDEPVL
                     TSMQILELLREIMPTQNIEELEREWDTDCAYEIPGTARFRVNGFRDMYGFGAVMRVIP
                     ELIPTFEELNLPEVLRDFCKLSKGLVIVTGPTGSGKSTTLAAMVDYINRTRNEHIITI
                     EDPIEFVHTPRQCIINQREVHRNTRSFTRALRAALREDPDIVLVGEIRDLETMEIAIE
                     TAETGHLVLATLHTNTAATTVERMIDKFPAERQNQIRSMLSDTLKGVVAQTLCKKIEG
                     GRIAVFEVLVVTTPVAALIREAKTHMLPSVMQTGRATGMQTFSDELTRLAAKGIISAE
                     DAYIKAVDKVEIETKFRLAGISLDFKKQAEEAARIARMAKAEEMLQAGRAALQVDPKN
                     IAALCDVAWILATSPFAELRDGKEAVKLAEKACSLSHNKDAAALEVLGAAQAEYGSFR
                     RALDATRRAAELYKQAGDVVRATALGNRLGMFEQNKPYRDE"
     gene            complement(9631..11040)
                     /locus_tag="PLZ74_05765"
     CDS             complement(9631..11040)
                     /locus_tag="PLZ74_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR97863.1"
                     /translation="MALNFWFGKKEAKSDQAMVGGVLLGTVGPVVLSEASADASASEA
                     PVPEASCPVAAGGPRAEPVVASVPVSDVPGLAPAALRSSSAEASAGSKVVLKAVGGGG
                     GVVRLRTPGTPAVAPAASGPAAAAPGAFGLQPRGGQPTAPEQAGAAPVPVASPAPPDR
                     AADTRQGTGVVRLRAPETPAGTPVASGPAAIKPGVFGLRPRGGQPAASEQAVAAPAPA
                     ASPAPPEKAADTRQGTDVVRPKSDQRALYYQLMNGLYDALLILDDQGHVVDCGRRTEE
                     LLGYTREEAWDLPVGKIVPGMTSQMFEHLKQNLAENHHILIDARCVRKDGSSFAGEVG
                     VSTLSLTRGTNMVFAVRNVERRKKAMEELRKSRAALDSSLAPTFVCDPEGLFLIVNRA
                     LLEAFGIPDEEQAKSLRFMDLLPDASRFFLRAVCGEKVRETLKVMLPNGASLKLELTL
                     QPVQSGQNVTAVAGSLLQA"
     gene            11167..12177
                     /locus_tag="PLZ74_05770"
     CDS             11167..12177
                     /locus_tag="PLZ74_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR97864.1"
                     /translation="MKKKRYTKNMLVRELAFSTGITQKTARFVLEALRHIAYRETADQ
                     GFTLPGLCRMDIIRRKARRMRNPQTGENILIGEHDALRVRALKKARDAITPQPKDLIT
                     TLPPEPSETAPQSKILDDFSNAVSFCCKKCGQEIEAPLAAAGMRAECPVCKEPVTIPN
                     ESEPGTLHGPALPEEPEPTPAPAPVPAPAPAPTPTSAPVPDTGAALPTPAAASANEPA
                     APEPPLPAIDHAKLKNQTIRIDLGALGFDTTGTPQPQKAPPRKRMISFFCKNCLQEIE
                     APAEMAGSPSECPSCGVSFEVPFFSDPGTLHGSDLETKRDAASIKEMKSRTIRIEVPD
                     DF"
BASE COUNT         2524 a         4201 c         3289 g         2164 t
ORIGIN      
        1 cgtctttttc gccccgtcct gccagaactc caagggaatc cgctcttccg gatcgagttt
       61 gggcagcagg ttactgttgt aatcggtggg cacatccttc agcgccacca ccgcgaacga
      121 ggggttcagc acctgcaatc caccgttata cagcgactta tagagccgga agaccggtcc
      181 gtacccatcc ttcttgatcc cgtagagaac cggctcgccc gatgtggcgc tccgggtcgg
      241 atccgccatg accgggatcc ggatcacgca attggtcagg ggcttgaaat aatcgttgcg
      301 gtagtggccg gtctgctccg cacaactcca gttcttctcc gcgccgaact ggccgtcgat
      361 ccggaccgca taagttccct ccgcaaaccc cgagggagga tcgatggtat cgaaccgcac
      421 cacagtgttc gagaaggtga cattccgtcc ctcgccaatg aattcgcact cctcgatggc
      481 atcgttccgc accgtgacat gaagcgtgtc gccgacctcc acaccggccg cggcgagtcc
      541 gtaggccgcg ccagccttgt tctggcccac atgatagggt gcggtcgcgg caccggaggt
      601 cgtgcgccac tcggcctcct gcggccaacc gctcacactg gccagaatcc ggtcgagccg
      661 cagggcctgg gacgtcgcgg ctttgttctt gcccttgacg gtaaaactca ccgtgtgttc
      721 gccgggcccg agcgacagac tgccgaaggt gatgcgatcc cgcgtgtttg gggtcggcgc
      781 gtatccgtcg aactgcgccg acgcatgcca gagcctgccg gtgaagaggt tctcgaacat
      841 cacggtggtc gaggcgctct cgcccgcgag acaggcggac gcgccgttgc ccgcggccac
      901 caccgcggcg atctgaccat agcggtccgc atacagcgca ttcggattgc ggttggcaaa
      961 gaccgtgcgc cgcatctgat ggggcgagtg gttccagacg tggtagacca cactttcgcg
     1021 ccagaggatg ttggtgccgc ccgccgccat ctccaccaat ccatccgtgc cagagcctgg
     1081 caaggcgaaa cccaccgcct gataaggctc ctgattctcg ggatagaaga tcaccgtctc
     1141 gcgggcggtg ttgcgcaact catagcggaa acgttcgatc accttgcgca catccatatc
     1201 cagcgacgcc tgggtgttga cccggttcga cttgtacatg aacagcagcc aggaacccag
     1261 ggtcatgccc atgagcaggc tgaacaccgc gaccgcgaag atcgtctcca gtaatgtgaa
     1321 ccctttgcgc atcacacacc tcgcatctag ggcgtccgtt tcaactcctc ggtgaacagg
     1381 taatccatcc gctcgaaatc caactcatcg aaccggcaga tgaacttccc ggttttaatc
     1441 ggatgaaaca ctttgcgcca gccccagcgg gagcaggtac agggataggt tgtgaccgtc
     1501 atatgagtca tgttggtggt cggcgaattc accgtcacct ccgttttccg gaggaagcgt
     1561 ccggccggat ccgccgcatt gccgaaatca tccacccgcg tgccgagttc gcggtacaga
     1621 tgcagatccg aatacggcac cccgcgcgcc cgttcaatcc ggccctggga gagcgatgcg
     1681 gccacgtaat ggtcccgcgc catctggaaa cgcgagatca tctggtcgac caacggaaac
     1741 accgccgtca ggataatgac cagcaggccc atcgccacca tcagttcgac cacggtcatg
     1801 ccgctctgat ctgttctgcc catacaccgc cccccgtcag gtttgccgcg acggcacctg
     1861 atcctcttcg atccgaagca tgtccttcgt atcctcctgc ccaggcaggt acggttccac
     1921 cgcatatgcc cgtttggcat tcatcttctg cagcaggtcc cgcaactgga tcacggcgtc
     1981 ataacacagg ttgattattt cctgccgcgc cttctcgtct ttgacgccgc ccatcttaag
     2041 catttcgatg ctggtcagca gcaccgtggc gggctggccc agcgaatggc acaccccggc
     2101 caagctctcc atcatcacgc ggttgcgctc cacctgactc tcggcgatct cggcgcgccg
     2161 acgctccgct gtgtcgcgca gcgagaccac catgccgcgc agttcattgt catcgttgat
     2221 atccggcacc gccgcgatct gaagccagtg cgcctgctcg ttgaccagca gccgctgttc
     2281 gccgatccag cttttgcgtt ggcggatgtt ttcgagcatc ggcatgatca cggctttgcg
     2341 gtggaaccag gtcgacagat tgaccccgaa cacctcggtc tcatccccgc ccgccagcag
     2401 atgcaccatc atgcggttgg cgtaaaccac cttgccctcc atatccgtga tgccgattcc
     2461 cgaatcggtg ttatccatcg cgtgatagac cgagagcagc cgctgctcgg cctgccaccg
     2521 cgactggatg tcgcggatga gataacagac cggctcggga acgcgtaacc cgttacccat
     2581 ggccacgatc tccacggcgg tgaggctgcc gtccctacgc accgcgaaag cctgaatacg
     2641 catgtaccgg ctctccgtga tcgccgccag aatttcctcc agcaacgaag aggtcatccc
     2701 ggccaccaac gtctccaccg gcataccggt caggccccgt tcccgttcgc cgtactggaa
     2761 gaaagaggtc gcgcgggcgt tcgcctccaa gacgatcccg tgccggtcag ccagcagaat
     2821 cgcatcgtaa gcgctctcga acagaaaccg gtaatccagc ttgccgaaat aatccgactg
     2881 aacctcgacc cccgagcggt cggccacggt ggaacgtccg taacccaacg agcggatatc
     2941 aattttgatg gtctgagatt tgtcattcgt actcatcgta atcgacacct ctgcgttaca
     3001 atccgttctg taccgatccc gtttttcggc tgaattacgg ttccgaaccg tttgctaaaa
     3061 atccgctgat gagtttacat catattttag caaaaatcat gccgaacccc ataccccata
     3121 tttggcacgc cgcaaacaat ctttttctcc cttctgcaaa gccgctttgc aaaaacgaga
     3181 atccgaaccg ttgccggaac ggcgtttcac gctcggtggc agaacccccg gaagagcgcg
     3241 tggaacatgc cccatccggc ctgagacggc atcccgtacc tttatcgcaa aactggcatt
     3301 gtttctgctt actttacccg tatggccacg gggttatcca tatccgcgct aacgcggcaa
     3361 tccaagccgc tcaccgatat tgtggggatc gaggtgggtt ccggcaatcc ggacggcgtt
     3421 cccgcagtgc ggttgcgcaa gcgcaacggc cggttcgaac tgctggccgc cggattcctc
     3481 cctctgccgg gaaccctgcc cttggatctg gagaccgcgc agaattccac gccggtctgg
     3541 ttgctgccgc gtccgtttca ggcgccgtac gccgccctgg cgctctcctc gccgctcgga
     3601 ttcctacggc atgccgcgga aacgggggag gagacgccgg acaagcatgc gacccccttc
     3661 cggaccgcct cccgggcctc aaccccggaa tccccggcgt tgctgaccgg cctgcccgag
     3721 ttccaggccg cctgggcggc ccgtctcctt cccgaaggcc gccgccccac cgcccgctcg
     3781 atccagttct ccccagccgc cgcactcaat gcgctgacca cctcgccagg cttcaaaacg
     3841 gtttccggca cggccatcgc gctctttgtc ttcgccgagt tcacctccat caccgccttt
     3901 caagaggggc aactgatgct gtaccgcgaa catgccgcag gctacctgca cctccgcaag
     3961 gttgtcagcc aacatatggg cattgacatg gctctggcgg actctctgct cgacgacacg
     4021 ctggtcgacc ccacgccgat catcgagccg gtgctccgtc cgctgttcag gcaggtcgag
     4081 atttcctcgg actttctgct ccgcagacgg aactgtaagg cggaccactt tttcctgtgc
     4141 ggcgtgcctg ccgggggccg ttactggtct tcggtgttcg cgcagatgat gagccatacg
     4201 ctcacgccgc tcccaccgct ggacggggcc gaagccctgc cccgctccaa cttcggcgcg
     4261 gagtccctct cgccgatccg gaaacagttg ctgatgaccg cgtacggtgc agcgcgcgcc
     4321 gccttggagg acgcatgagc aacgtatacc acctcaatct gctgcagccg gaagagcacc
     4381 tgagttccag ccccatccgt ctccgggtga tgctcccgat gttcgccctg ctgagtgcgc
     4441 tcggcgtggg ggtctggtgg agcgtgctgg cgggccgcgt ccatgcggtc acccagcaga
     4501 aaagcgccat tgaagccaat ctggcgcaac tgaaaccgac gcacgagacc gtgctcggat
     4561 tgcgcaccga acaacatgat ttgacggcca cgctggagca gcttgcctat tacacgcggg
     4621 cgcaactgcg cttcggccct tcgcttcagg cgctcgcgca tcatgttccg gaccagatcc
     4681 agctgaccga acttcatatc ccgccgcccc cggaaccgcc gccgcccgac cccaaaaacc
     4741 cggtctgggg ccccaccaac ccgatcgagc gcgtcaccct gcgcctgacg ggacggacca
     4801 ccggtgcctc acaggtcgaa accctgttgc gcgccttccg taccgaggcc ttcaccaacc
     4861 tgatccgttc agcgcaaatt cccaagggcg cctaccgtca ggacacaacc cgacgcggcg
     4921 cggccaaaga gacgctcctg tttgagttga actgcgaatg cgcactcagg aggttcgaat
     4981 gagcttaaac acctccataa tccataaaat cgccccctcc atgcagcgct cgattctgat
     5041 caccctcggc gggctcatga tcgccggtgc gatggtcttc ttcttggtgt tgcccgcgcg
     5101 cgcgcgcctc caaacgctcg ccgctgaact gaacaccctc cgcgacaccc tcgccaccgt
     5161 gcgcgcggat atcgcaggca cagagcagca acggctgaag accgccgagg tccgctcgaa
     5221 acacgacgcc ctggtccgat cgggtgtgct tcagccgctc ctcaactcgt tcgccatgcg
     5281 gggcaagtcg ctgctcgatc cgctcgccca gcaaaccggg tttgcgatcg ccaacgtcaa
     5341 ggaagaccgt tttctgcgac tgcaagtgcc aaaacaggtg cccctccatc tctatggacg
     5401 ccagatgatc gagttctccg ggcagggggc ctacacctca atcgtgaact tcatcgcatt
     5461 ggctgaagaa cggttccccc tctgtaccct cgcgggactc aagatcatca gccaaaacca
     5521 gactccggag acgcacaagg ctttcatcac cttcgaatgg cccgccaaag gcgagaaacg
     5581 aaccccatga gaaacgatca gcttccaact ctcaacgctc aacgctcaac gactcttccc
     5641 cgggagccgg gtgctcggta tttgacgtta aacgttgaac gttgcgtgtt gagcgttctt
     5701 gcccttttcc tgctcgccgc cttgccggct cgcgccgcgc gcgacccctt ctggcccatc
     5761 ggctacgaac cgccgaaacc cgaacccgtg gtcaaggaaa tccccgaagt gccgaaaaag
     5821 acggtgccca aacccgtccc gcccaaaccc aagcccccgg ccgtgaaacc cgtgaccgac
     5881 ggagactgga ccgacgcgcg tgccttcatc acggtcaccg ggtttacccg cgcgacacgc
     5941 cccgacacag gtgaaacccg caccctggct ctgatcaacc gccgctccta cgcggccggc
     6001 gacacccttt gcatcactaa cgccggcatc gttttcctct ggcgtatcga ctcccttgac
     6061 aaccgcgaac tgaaactggc tccgatccaa gccacccgga tcgtcccgcc tgtgtcggga
     6121 ccctccggaa gcgcaccctg acgcgatttt tccatgtatt ccatcactgt cgattcacaa
     6181 cccaaaacca ggagcatcca catgaaccgt atcacccgac tctgtgcgtt catcgcaacc
     6241 ggcctgtgtc tggcctggca gcccgtcaca tgcgcccaag aggctgcggc ccaaacgccg
     6301 cagcccccca aaaccaccgc ctcggtccgc gtcaccgatg cctcatcccc cagcgagccg
     6361 ctcgtctcca tgaccttcga cgaggcgccc ctcgcggatg tgatcaaggc tttccgtgac
     6421 gccacggggg ccaacatcat ctccggcggc accaatgtgc tgcaggcgaa catcagcgtc
     6481 agtctcgaca atgtgccgtg gcgtaaaggg ctctcctcga ttctggaccc tcaaggcctg
     6541 caactggtcg aacagcccac cggatccggc atttacgtga tcaacacgaa aaccgtcgag
     6601 gtccccaaga tcacccgcgt gttctcgctt gagtacatca aggccgagga tgtcgccgac
     6661 ctgctgaaaa ccaccctcgg caccaccggc gggatcgtga ccccctttaa agagtccaat
     6721 gcggtcatcc tcaccgccac ggaacagcag atcagcgagt gcgaatccat catcaaggcc
     6781 atcgacaaag cctcgccgca agtctacatc gaggtccgtt tcgtcgagct gaccgcgcaa
     6841 gccagtcgga aactaggcct gaaatgggat agcctcggca ccaaagaagg atggggtgcc
     6901 catgccgacc ctaccttctt caacttcgag aacgattatc tcaaaacgaa agacaaaggt
     6961 atggaccgca gccaaaattc cgagcgtgtc cagccgggtg acacaacgtg gcgggattcc
     7021 ctgacccgca ccctcaatcg caacgacagc tccacccaga cgcgcacctt taccggcacc
     7081 ctctcgctgg acgccttctc cctcaccatg aacgcgttcg aacagatgga cggggtttcg
     7141 atcttctcca atcccaagat cattgtcgcc aatgaaaaag aggcgctggt ggacatgacc
     7201 accaaggaac ccaacctgac cgtgacctcc tcccgtacgg gaaccgccgg cgaccaactc
     7261 gacatctcgg ccgaactgga ggtcatccct ggcgagaaag gggacaagga cggcaaaggc
     7321 aagggcattt tcgcgggtga ggcgttcttc agctacggta tcaccctcaa agtcacgcca
     7381 cgcatcagct ccaccggtct gatcaccctg aacgtggaac ccacgatcag cgatcgtaag
     7441 agctattacg agttttccgg cattgaggag accaagactc ccaccccgaa atacccgatc
     7501 atctacatgc agcgcctcca gacggttttt accatgcagg acggttccac ggccgtgatc
     7561 ggcggcctga ccacgacaac cgaggataac atcgattccg gcatcccctg gctgcgtgag
     7621 ttgccttggg tgggaccacg cctcttcggc tggaagagcc gcaggaaaga acagaaggaa
     7681 atcattatct tcgtttcggt cggtctggcc gaccccctca agttgcctga gtccgtcggt
     7741 atgccgaaaa acgccattct cgggcgcgac ctgctgaccg gtgaactaaa agagccgggc
     7801 gaccgcacca aagaagaggt gatgagcctc cgtgatcccg tgcacgggac caaagcgcaa
     7861 ccatccacag aagaggtcgc cgtacccgcc gcagtcgctc cggacgtggt ggtgaacgaa
     7921 aaagaggcgg ccaaaccggt aacacaacaa cagccggtcg ataccgcccc gctactgcaa
     7981 aattagaagg gaacctgtca gaacggtccc ggcggcgcag gccctcccgc gccgccggga
     8041 acaaaggtcc gcttcaccga agcctcccag gctgaatccc tgttgatgtt gtaaaccccc
     8101 gaaccgcact ccccgtcagg actcccatgg ccacactcga ccgcttcctc attccgctcg
     8161 tcaccaagtc aggcagcgac ttgcacctca gcgcgggccg tccgcccgtc atgcggatcc
     8221 acggcaccct ggcaccggtt ccggacgaac cggtcctgac cagcatgcag atcctggagt
     8281 tgttgcggga aatcatgccg acacagaaca tcgaggaact cgagcgcgaa tgggacaccg
     8341 actgcgccta cgagatcccg ggcacggcgc gtttccgcgt caacgggttc cgcgacatgt
     8401 acggattcgg cgccgtgatg cgcgtgatcc ccgaactgat cccaacgttc gaggaactca
     8461 acctgcccga ggttctgcgc gatttctgca agctctccaa agggctggtc atcgtcaccg
     8521 gccccactgg cagcggtaaa tccacgactc tcgcagctat ggttgattat atcaaccgga
     8581 cacgcaacga acacatcatc accatcgagg accccatcga gttcgtgcac acgccgcggc
     8641 agtgcatcat caaccagcgt gaggtgcacc gcaacacacg cagtttcacg cgggcccttc
     8701 gcgccgctct gcgcgaagat ccggacatcg tgctcgtggg cgaaatccgt gatctggaga
     8761 ccatggagat cgccatcgaa accgcggaga ccggccatct ggtcctcgcc accctgcata
     8821 ccaacactgc agccaccacc gtagagcgca tgatcgacaa gttcccggcc gagcgccaga
     8881 accagatccg ttccatgctc tccgacacgc tcaaaggcgt ggtggcgcaa accctctgca
     8941 aaaagatcga gggtggccgg atcgcagtgt tcgaggtgct ggtcgtcacc acgccagtcg
     9001 ccgcgctcat ccgcgaggcc aagacgcaca tgctcccctc ggtaatgcag accgggcggg
     9061 ccacgggcat gcagaccttc agcgacgagc tgacgcggct cgcggccaaa ggcatcatct
     9121 cagccgaaga cgcctacatc aaagccgtgg acaaagtgga gatcgaaacc aaattccggc
     9181 tcgcgggcat cagcctcgac ttcaagaaac aagccgagga ggccgcccgt atcgcacgca
     9241 tggccaaagc cgaggaaatg ctgcaagccg gacgcgcggc gctgcaggtc gaccccaaaa
     9301 acatcgcggc cctgtgcgat gtggcgtgga ttctcgccac gtcacctttt gcggagttgc
     9361 gtgacggcaa agaggcggtg aaactcgcgg agaaagcctg tagtctgtca cacaacaaag
     9421 atgcggccgc gctcgaggtg ctgggcgcgg cccaagctga atacggatcc ttccggcgcg
     9481 cgctcgacgc tacgcgccgc gctgccgaac tctacaaaca ggcgggtgac gtcgtacgcg
     9541 caaccgcact cggcaatcgc ctcgggatgt tcgagcagaa caaaccctat cgggatgagt
     9601 gaggcgctcg ctccggcgcg gcgcggagac tcaagcttgt aaaagcgagc cggccacagc
     9661 ggtgacgttc tgaccgcttt gcacgggttg caacgtcagt tccaacttca gcgatgcgcc
     9721 gttcggcagc atcaccttga gcgtctcccg gaccttctcg ccgcaaaccg cccgcaggaa
     9781 gaaccgtgag gcgtccggca gcagatccat gaaccgcaga cttttcgcct gttcctcgtc
     9841 cggaatcccg aacgcctcca gcaacgcccg gttcacaatc aggaaaagcc cttccggatc
     9901 gcagacgaac gtcggcgcca acgacgaatc cagcgcggcc cggcttttgc gcagttcctc
     9961 catcgccttc ttccggcgct cgacattgcg gaccgcgaaa accatattcg tgcctcgggt
    10021 cagggaaagc gtgctcaccc ccacctcgcc cgcgaatgag gagccgtctt tccgcacgca
    10081 ccgcgcgtca atcaggatat gatggttctc agccagattc tgcttcagat gctcaaacat
    10141 ctggctcgtc atacccggca caatcttccc caccggcaga tcccacgcct cctcacgcgt
    10201 gtaccccagc aactcctccg tccgtcggcc gcaatccacc acatgcccct gatcatccaa
    10261 aatcagcagc gcatcgtaca gcccgttcat caactggtag tagagagccc gctgatccga
    10321 cttgggacgc accacatccg ttccctgccg cgtatccgca gccttttcag gcggagccgg
    10381 tgaagcggcg ggagccggtg cggcaaccgc ctgttccgac gccgccggct gtcccccccg
    10441 cggccgtaac ccgaacacac cgggcttgat ggcggcaggc cccgaagcca ccggcgtgcc
    10501 tgccggggtt tcaggagccc gcagacgcac cacacccgtt ccctgccgcg tgtccgcagc
    10561 cctgtccggc ggagccggtg acgcaacggg aaccggtgcg gcacccgcct gttccggcgc
    10621 cgtcggctgc cccccccgcg gctgcaaccc gaatgcgccg ggcgcggcgg cggcaggccc
    10681 tgaagccgcg ggcgcaactg ccggggttcc gggagtccgc agacgcacca ccccaccacc
    10741 gcctcccact gccttgagca caactttgga accggcggag gcttccgcac tacttgagcg
    10801 caacgccgcc ggagccaaac ccggaacgtc gcttaccggc acagatgcaa caaccggttc
    10861 ggcacgcggc cctcctgccg cgacaggaca agaagcctcc ggaaccggtg cttcagaagc
    10921 agacgcatcc gcagacgctt ccgaaagcac gaccggaccg acggtcccca aaagcacgcc
    10981 cccgaccatc gcctgatcgg acttggcctc tttctttcca aaccagaagt tcaacgccat
    11041 gatttccttc ctgatatgct aatcagcagg atcaatgcca agacaagcct atcaaacatc
    11101 ccgtctttgg cacaacttct gctaaacaat gtaggattat tatgagtttt ccaataggcc
    11161 tttgcaatga aaaaaaaacg ctacacgaaa aacatgctgg tgcgcgaact ggcgttcagc
    11221 acaggaatca cccagaaaac cgcgcgattt gttttggagg ccttgcgcca catcgcgtat
    11281 cgcgagacgg cggatcaggg tttcacgttg ccgggattat gccgcatgga catcatccga
    11341 cgcaaggcgc gccggatgcg gaatcctcaa accggagaaa atattctcat tggtgagcat
    11401 gacgccctgc gggtgcgcgc gctcaaaaag gcgcgtgacg cgattacgcc gcagccgaaa
    11461 gacctgatca ccaccctccc tcccgaaccc tcggagacag ccccgcagtc gaagattctg
    11521 gatgatttct cgaacgcggt ctcgttttgc tgcaaaaaat gcggacagga gatcgaggcc
    11581 ccgctcgcgg ccgcgggcat gcgggccgaa tgtccggttt gcaaagaacc ggtcacaatc
    11641 cccaacgagt ccgaaccggg aaccctacat ggcccggccc tgccggaaga accggaaccg
    11701 acacccgcgc cggcaccggt accggcaccg gcaccggcac cgacaccgac atccgcaccg
    11761 gtacccgaca cgggcgccgc gcttccgacc ccggctgccg catccgcaaa cgaacctgcc
    11821 gcaccggagc ccccgcttcc cgcaatagat catgcgaaac tcaagaacca gacgatccgc
    11881 atcgacctcg gcgcactcgg gttcgatacc accggcacgc ctcaacctca gaaggctccg
    11941 ccccgaaaac gcatgatctc ctttttctgc aagaactgcc ttcaggagat cgaggccccg
    12001 gccgaaatgg ccggttcacc ttcagaatgc ccctcctgcg gcgtcagttt cgaagtcccc
    12061 ttcttctcgg atcccggtac gctgcacggc agcgacttgg agacaaagag ggatgccgct
    12121 tcaatcaaag agatgaaaag ccggaccatc cgcatcgagg ttcccgatga tttttgaa
//
LOCUS       DAOSRB010000078        12046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5274_length_12046_cov_6.913852, whole genome shotgun sequence.
ACCESSION   DAOSRB010000078 DAOSRB010000000
VERSION     DAOSRB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12046
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5274_length_12046_cov_6.913852"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLZ74_05775"
     CDS             complement(<1..223)
                     /locus_tag="PLZ74_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97865.1"
                     /translation="MSYYNQNETNKRIAAVQAILDKKNLDAALVYFDELNVANGWYLT
                     GWCGQFEKGSVFVPAKGEPWLLGGPESEPF"
     gene            complement(234..608)
                     /locus_tag="PLZ74_05780"
     CDS             complement(234..608)
                     /locus_tag="PLZ74_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOR97866.1"
                     /translation="MSVISMEIGRKQWVFADGDLPPRGDSEPFGHEALMIVNNGEQDA
                     TIKVTILFEDKEPKEDITLHVPARRVKCFRMDYPLGDEQYQLGPGQTATILASDVPVV
                     AVYGRLDRRKDMAYYPVQGFSC"
     gene            complement(702..3938)
                     /locus_tag="PLZ74_05785"
     CDS             complement(702..3938)
                     /locus_tag="PLZ74_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97867.1"
                     /translation="MTVRKIGTTLIAMIIGSVLADEMLENELVSLTFDGQGCLTSIRE
                     RQSGRELVREKSPFVSVVNAEGKTLVPTSCAKDAKGNLCFGFAEAGEMVLAVRPFSGG
                     WSLGVERQTVQDAQEIHIGRVHPDCLKYRGHMVNAYSDERSFVCVRAYDVGASFRCAE
                     GLVEASVKTSARPLTGARFGFAAGPRSAAIPALRAMTIDADVPCSDRGGAWALGAEAC
                     RRSYWMAWGIGPFTIDDEIEQCRRGGFGTLHFDHFFKSYGSYELPGDGSGRDGMTLLK
                     EMIAKAHDAGLHCDFHSLCACVARNDPIATPECQDGLMVTAAYTLGTDLEAATNVTTL
                     VVNEKPVDCHEFETTAWGKSNTLKIGTELVTYTGRSTEAPWTFTGVTRGAFGTTVKPH
                     AKGTKVEYLFNCFSCFQIDADSELAKRAWELQGKLFGAGFDNYYLDGADCIRDKRKTD
                     KFVRELYKAAQANGKTPLFEDSLWTSAAWWFHSRIGANDYVQWGSKRSLDRRFERLVS
                     QARDANFIEPEMGWWATLQGNGAQGNPYRIDEHEYFGRKCAAYDAAMSVLPGNAYPAR
                     YMVARNIHRYVTVIGWYERFRLAYAFNDETLAKFRVKRDEYRLRQDTDGVWKVRPLDF
                     FHHRVTGPDARAWRIDSKERREATLRIEALLTCKPWDDPEAKVLLDPACAATFEKSAA
                     KGVTVECANSQDSERGHVVTITANNANATSRGAWSCVARVWPKPPVGQPPVYGDCSGS
                     RGFGLWVRGDGSGALLNVQLMGAKGAGSGPKSEHYQRLDFTGWRYLEFNRRERDSGAA
                     SDFVWPYVEKNSSIDYSLERVNVSKLQAVNLYLNEIPAGGKTEVSVSAVKLLPERKQV
                     TYSGLAVTLNGTRYACPFALEPTDFAEMEDGCWTRYDASANPLERAPADLAELRAGTN
                     ELTFEGNGGEGVNVRAEVNILALGEPAAALKPTVNAAAHEALAYEVVEPATWAPSKGF
                     EKIPDLVTRPGEKAALSFEVYGPTAPFTLVVGDEARRFDCTLSPKEYLVCRDGEHWQL
                     KRRGEKPCIVASGKIRPFAPFTGSRKVAFSSDDPEQANVFLIFAKHYR"
     gene            complement(4018..4797)
                     /locus_tag="PLZ74_05790"
     CDS             complement(4018..4797)
                     /locus_tag="PLZ74_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR97868.1"
                     /translation="MKRAIVFNIEKFATHDGPGIRTVVFLKGCPLRCMWCHSPESWSF
                     EVERYPDGEVIGREMSSEDVLAEVLKDKDFYDVSDGGLTVSGGEPLARPAFLAELLGK
                     AKAAGLHTAVETSGAATEKVFEPLLPLVDLWLWDVKKLDADKHFTYTKRPLAQILANL
                     RRVNDALAAESPRKRSIVLRCPMIPGINDGAEDIEAVGRLADSLPAVIELHVAPYIPY
                     GIDKARRLGLKVYEAPQPPPEYGRRIVAALTRHTAKPVRLP"
     gene            complement(4809..7190)
                     /locus_tag="PLZ74_05795"
     CDS             complement(4809..7190)
                     /locus_tag="PLZ74_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOR97869.1"
                     /translation="MKVKRREFMGSAAAFFAALGLDAPAALSAARERPFAAADYETFR
                     KSLRAFYPDECHGKCRDPRQVASVRMIGNELDAYVAAHPDCDVLDIRRESYLAMRRHF
                     VPYILPGSPFYCEAGVNGGWCVWQGAESVPGRHINRICRGWLEERYIPREAFARLRAR
                     RRGCLMLTGDFVDDMHHIPPFHTIFTKGFRGVRREVETALASCPAEDASGRKGLEVAL
                     VGLDTVHEIQLAFVREAERQLAAVGLSAEDRLRLERIAVAARRCPWEPPRDFYEGLNT
                     LWFVREILGYVEGTLNFSLGRPDAWLIDFYRRDLAAGRLTEPEARDLIARFLVMADCH
                     LDTRLEIDGYSDQEAEIPLTLGGCDAEGRPVYNELTAAFLDAHIGLDLVFPKLHCRIS
                     EKSPQEYLEKIGAMLMRGHAVFAVFNDDRHIPNFMALGYPVARARDYVCCGCWDGNVD
                     SDTNLDTANYMSVARILELTIYRDPNFEREARITIDPVDDATSFEQVRDIVYHNFIRF
                     LRDVLADYTRYGGENVHVSARPVYSSCLTGCVAARRDALAGGAAFSPRIITLAFLGNV
                     VDSLCAIRKICFEDRMATLPDFLAAVRSNWKCPGGEALRQAVLAAPYWGDNSEASNGL
                     MRYWIDSVSEDIRDLKNEKGGPYVLACWIYREFLLWGLKMRATPDGRYDGDRLAQGFA
                     PSEYRCKAGVTTVFNAIGSLDHSRLYASNANLAFEKSAMNAELFAAVFRVACRKGMHL
                     LQPNCYSVEDLLDAQVHPERHQNIMIKVCGFSARFVALSKRWQDEVIQRHRLH"
     gene            complement(7318..10494)
                     /locus_tag="PLZ74_05800"
     CDS             complement(7318..10494)
                     /locus_tag="PLZ74_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97870.1"
                     /translation="MQLNMLGKWICALSVAVTFSVCGSTWTGGGAAGDWNDSSNWDGG
                     VPVADSLADFTGDVVISSAFSLPGKVTIRVAANKRVDFNGVISGTGPLVVGEKGSASN
                     YGSVYFNATNAFTGNLEVLHGFVYGTVDNAFGAADAGRIMVNPDQTAVSRTQLYYKGI
                     RSLRTLEFTLVDQKTVTFDGVTTVGTIVKKGNFRGTINGKLFVTGASNLDSLLLFSFA
                     EGAELVFTNNPSVLLPYVYTHKGKVILASVSNTVNRCTSGPYAPLGYPWSRIAFTLRC
                     DVNGAITESSALCFEATDNTFYPGYSKLDLNGTTQSVRRLTTYTDPSWDKWNDVTRLG
                     RVTSSVGGLITVNDGVDFINRNVFEGTASLEMKLASGITGTLLAKSTSSGELIATSGT
                     TVLTNGAQWVGTVRVGNGAVLKTCSADAFSNGVARIFLEGGTLELAEGSYSVKELRDA
                     TGTSLAFGWYAATASGEIKACEGLSGAGVIFVAPPDDPPEQAWTWCGGGVDDKVTTLG
                     NWKNATELPDFGYTGNVFSFPSNATVELDVPVNAKSLVFDEDASGTVEFTSTPEANGR
                     LMLANGVIDIRPGTSDRVKKIVFKSPFKFGGDLAASVPKGSSLSFESSFDSAGGASFE
                     KTGYGELRITGNANKIYGDVTVRTGHTYLTGLDPLGGTGALYIYPEAEKHKETALYLS
                     NAVVNRPVSRINPDGHNQLYALASTTNVLAGLVSLQNGFFTFTVQSKARLDFAGDFML
                     ITQRLLYPSLVDDASEVRFTSPHLNDNDAVLYGASSELNNYARFVLGASGSKFATDYN
                     FKICCTHVYSAPNVFSNAVPIQIVSPKARVDLGGWRQAVANCCCEGVLTDGVLTACGG
                     DVSAANGGTIFGASGSKLVLTDAIPCQGTSNADTGIMPVFGGAASVERAGAGTNTFRR
                     ANISTGEVSVVAGRLVFAAAGDQWDFWQKVNNVWTRYPQTSATAGTWAGKKATVSGGE
                     LVIGHSYAFPKDAEFYLEGGTLELASGVVQQIGSLWLKKNGDWVKCKDGFYGSATNLS
                     VPPENRRALITGDGTLLAGRVGLAIILK"
     gene            11023..>12046
                     /locus_tag="PLZ74_05805"
     CDS             11023..>12046
                     /locus_tag="PLZ74_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOR97871.1"
                     /translation="MTDSKAKRVGVLVELTREFGRDLCKGITDFACSHEGFIPFFITP
                     ETLKRKSELKSYDGFIARVMSDEMAATLAALKKPIVDMYYDKPRPGFAIVKTNHSRIG
                     RLAAEHFLERKFKNFAFCGFAGGRFSNYCSRAFQRALAAKGHRCHSYQPDARAKYEFD
                     STVLINEQLNRAPDAKALAKWIGLLPKPVAVFCPNDLRAWQLLQVCKECDIHVPHDIA
                     ILGLDNDVIVCGFSNPMISSIDPDTPAIGREAARTLVEMMNDPDFRKKTLIRQVNPNR
                     VIGRASTEVYPIDPPWLSDALVYMRKRVSDQLTADDVFKHLGLSHTTVNRTFRKVLNT
                     TVQKEIA"
BASE COUNT         2624 a         3853 c         3206 g         2363 t
ORIGIN      
        1 cgaacggctc ggactcgggc ccgccgagga gccacggctc gcccttcgcg ggaacaaaca
       61 cggagccctt ctcgaactgg ccgcaccagc cggtcaggta ccagccgttg gcgacgttca
      121 gttcgtcgaa atagacaagc gcggcatcga ggttcttttt atcaagaatc gcctgcacgg
      181 ccgcaatacg cttgttcgtc tcgttctgat tgtaatagga cacttttctc tccttagcag
      241 ctaaaccctt gaactgggta atacgccatg tccttccgac ggtcgaggcg accgtaaacg
      301 gccacgacgg gcacatccga cgcgagaatc gtcgcggtct gtcccggtcc gagctggtac
      361 tgctcgtctc cgagcgggta atccatacgg aagcacttga cgcggcgcgc ggggacgtgc
      421 agcgttatgt cctccttcgg ttccttgtct tcgaagagga ttgtgacttt gattgtcgcg
      481 tcctgctccc cgttgttgac gatcatcagc gcctcgtgtc cgaacggctc cgaatcgccc
      541 cgcggcggca gatcgccgtc cgcaaacacc cactgtttcc ttccgatttc catgcttatc
      601 acagacataa tctctccttt gcttaactat aatttaagaa aagcctttga agccttcaat
      661 tgtaaaatct gcaacaaccg cttaaatatc tgcaacaaaa atcaacggta gtgctttgca
      721 aagatcaaga agacattcgc ctgttccgga tcatcactcg aaaaggcaac ttttcgagag
      781 ccggtgaacg gagcaaacgg acggatcttt cctgaagcaa cgatacaagg cttctcgccg
      841 cggcgtttca gttgccagtg ctcgccatcg cggcagacga gatactcttt gggggacaac
      901 gtgcagtcga accgacgcgc ctcatcgccg acaaccagcg taaacggggc tgtcggaccg
      961 tagacctcaa aggaaagagc agccttctca cccggacggg tcacgaggtc cggtatcttc
     1021 tcaaaaccct tggaaggcgc ccacgtcgcc ggttcaacga cctcgtaggc gagagcctcg
     1081 tgcgccgcgg cgttaaccgt cggcttcaga gccgccgcag gctcgccaag cgcaaggata
     1141 ttgacttctg cgcggacgtt cacgccttca ccgccgttgc cctcgaaagt aagttcattc
     1201 gtgccggccc tgagttcggc aaggtctgcc ggtgcccgtt ccaacggatt agcgctcgca
     1261 tcgtaccggg tccaacagcc gtcttccatc tcggcaaagt cggtcggttc cagtgcaaac
     1321 ggacaggcat agcgagtgcc gttcaacgtg acagccaatc cggaatacgt cacttgctta
     1381 cgctcgggca acagcttgac agcactcact gacacctcag ttttgccgcc ggccgggatc
     1441 tcgttcaaat agagattcac cgcctgcaac tttgaaacat tcacgcgctc aagcgaataa
     1501 tcgatcgaag aatttttttc cacatagggc cagacgaagt cactcgccgc acccgaatcc
     1561 cgttcacggc ggttgaactc caaataacgc cagccagtaa agtcgagcct ctgatagtgt
     1621 tcgctcttcg gtccgcttcc ggcccctttc gcccccatga gctgtacgtt caagagcgca
     1681 cccgaaccat cgccccgtac ccacagcccg aacccgcgcg aaccgctgca atcgccatag
     1741 acaggcggct ggccgacggg cggcttcggc cagacacgcg ccacacacga ccaagcaccg
     1801 cgtgacgtgg cgttggcgtt gtttgccgtt atcgtcacca catgaccacg ttcggaatcc
     1861 tgagagttcg cacattcgac cgtcacaccc ttggcagcgg atttctcgaa ggtggcggca
     1921 caagccggat caagcaaaac tttcgcctcg ggatcatccc acggcttgca tgtcaacaac
     1981 gcctcgatgc gcaacgtcgc ttcacgccgt tccttcgagt caatcctcca tgcgcgcgcg
     2041 tccggtcctg tcacgcgatg atgaaagaag tcaagcgggc gtaccttcca gacgccatcc
     2101 gtatcctgcc gcaaccgata ttcgtcgcgt ttgacgcgga acttcgcaag ggtctcgtca
     2161 ttgaaggcat acgccagccg aaaccgttca taccatccga tcacggtcac ataacgatgg
     2221 atgttgcgcg ccaccatgta tctggcgggg taggcgtttc ccggcaggac gctcatcgcg
     2281 gcgtcatagg ctgcgcactt gcggccgaag tattcgtgtt cgtcaatacg gtagggattt
     2341 ccctgggccc cgtttccctg caatgtcgcc caccacccca tttccggctc gatgaagttg
     2401 gcgtcacgcg cttgactgac aagccgttcg aagcggcggt cgagagagcg cttggaaccc
     2461 cattgcacat agtcgttggc accgatgcgc gaatggaacc accaggccgc cgacgtccac
     2521 aggctgtcct cgaaaagcgg cgttttgccg ttggcctgcg cggctttgta gagttcgcgc
     2581 acgaacttgt cggtcttgcg tttgtcgcga atgcaatcgg caccatcaag gtagtagttg
     2641 tcaaaacccg caccgaaaag tttaccctga agttcccacg cgcgcttggc caattcgctg
     2701 tcggcgtcaa tctggaagca actaaagcaa ttaaagaggt actcgacctt tgtgcccttt
     2761 gcgtgcggct tgaccgttgt gccgaatgcc ccgcgcgtga cacccgtgaa cgtccacggg
     2821 gcctccgttg aacggccggt atacgtcacc agttccgtgc cgatcttcaa ggtgttcgac
     2881 ttcccccagg cggttgtctc gaattcgtga caatcaaccg gcttttcgtt gacaacgagc
     2941 gttgtcacgt tcgtggcggc ctccaggtcg gtgccgagcg tgtacgccgc cgtcaccatg
     3001 agtccgtcct gacactccgg cgtcgcgatg ggatcgttgc gcgcgacaca ggcgcagagc
     3061 gaatgaaagt cacaatgaag ccccgcgtca tgagccttgg cgatcatctc cttcagaagc
     3121 gtcatcccgt cacggccaga gccgtcgccg ggcagctcat aactcccgta ggatttgaag
     3181 aagtgatcga aatgcagcgt gccgaagcca ccgcgtctac attgttcgat ctcatcgtcg
     3241 atcgtaaatg gaccgatccc ccaggccatc caatacgagc gccgacacgc ttccgcacca
     3301 agcgcccacg cgccgcccct gtcgctgcag ggcacgtccg cgtcaatcgt catcgcgcgc
     3361 agcgcaggta tcgccgccga acgcgggccc gccgcaaacc cgaagcgcgc gcctgtcagc
     3421 ggacgcgccg atgtctttac ggacgcctcc accaggcctt cggcacaacg gaacgaagct
     3481 ccgacatcat aggcgcgcac gcagacgaac gaccgttcat ccgagtaggc gttcaccatg
     3541 tgcccccgat atttcaagca gtcggggtgc accctgccga tatgaatctc ctgcgcatcc
     3601 tgtacggtct gccgttcgac gccaagcgac cagcctcccg aaaacgggcg caccgccaac
     3661 accatctcgc cagcctcagc gaagccgaaa cacaggttcc ccttggcatc cttcgcacat
     3721 gatgtcggca cgagcgtctt tccctcggcg ttcacaaccg acacgaaagg cgatttttcg
     3781 cggacaagct cgcggccgct ctgacgctcc ctgatcgacg taaggcagcc ctgcccgtca
     3841 aaagtcagcg agaccagttc gttttccaac atctcgtccg ccaaaaccga tccgattatc
     3901 atcgcgatca gcgttgtgcc gattttacgt accgtcatgc acttacctcc tgtaggagag
     3961 accgttaaca tgccgcgccc cctggccggc acctagtatg cggaaccagc cggatcgtca
     4021 cggcaggcgg accggtttgg cggtgtggcg ggtcaacgcg gcaacgatcc gccgaccgta
     4081 ttcgggcggc ggctgcggcg cctcgtaaac cttcagcccc agacggcgcg ccttgtcgat
     4141 gccgtaggga atatacggcg cgacgtgcaa ttcaatgacg gcgggcagcg aatccgcgag
     4201 ccgcccgacg gcctcgatgt cctccgcccc gtcgttgata ccgggaatca tcgggcagcg
     4261 caacacgatc gagcgtttcc gcggagactc tgcggcgagt gcgtcgttga cacggcgcag
     4321 gttcgccaaa atctgcgcga gaggcctctt ggtgtacgtg aagtgcttgt cggcatccag
     4381 ttttttcacg tcccagagcc acagatccac aagcggcagc aagggctcaa agaccttctc
     4441 cgtcgcggcc ccgctcgtct ccaccgccgt atggagccct gccgccttgg ccttgccgag
     4501 gagttccgcg agaaacgcgg gccgggccag cggctcgccg ccggacacgg tgaggccgcc
     4561 gtcggacacg tcgtagaagt ccttgtcctt gagcacttcg gcgagcacgt cttccgacga
     4621 catctcccgt ccgatgacct cgccgtccgg atagcgttcc acctcaaagc tccagctttc
     4681 gggactgtgg caccacatgc accgcaatgg acagcctttg agaaaaacca cagtgcggat
     4741 gccggggccg tcatgcgtgg cgaacttctc gatgttgaaa acgatggcac gtttcatccg
     4801 gtgttccctc aatgtaggcg atggcgctgg atcacctcgt cctgccagcg tttcgagagc
     4861 gccacgaaac gcgcggagaa tccgcacacc ttgatcatga tgttttggtg ccgctccgga
     4921 tggacttgtg cgtccaaaag gtcttcaacg gaatagcaat tcggctggag aaggtgcatc
     4981 cctttgcgac atgcgaccct gaacacggcc gcgaacagtt ctgcgttcat cgccgacttt
     5041 tcgaaagcaa ggttggcatt tgacgcataa agccgcgaat ggtcaagaga gccgatggcg
     5101 ttgaacaccg tcgtgacacc cgctttgcac cggtattcac ttggcgcgaa cccttgcgcc
     5161 aagcggtcac cgtcataacg gccatccggc gttgcccgca tcttcagccc ccagagcagg
     5221 aattcccggt atatccagca ggcaagcaca tacggtccgc ccttttcgtt tttcaagtct
     5281 cggatgtcct ccgacacgga gtcgatccag tatcgcatca gtccgttcga cgcttcgctg
     5341 ttgtcgcccc aatacggtgc cgccaatact gcctgacgca acgcctcgcc tcccggacac
     5401 ttccagttcg acctgacggc ggccaaaaag tcgggcagcg tggccatacg atcctcgaaa
     5461 cagatctttc gtattgcgca aagcgaatcc acgacgttcc ccaagaaggc gagagtgatg
     5521 atacgcggac tgaaagccgc tccgcccgcc agggcgtcac gccgcgccgc cacgcaaccc
     5581 gtcagacacg aagaatagac cggccgcgcc gacacatgga cgttttcgcc gccataccgc
     5641 gtatagtcgg caagaacgtc ccgcagaaaa cgaatgaagt tgtggtaaac gatatcgcgc
     5701 acctgttcga agcttgtcgc gtcgtcaacc ggatcaatcg tgatccgcgc ctcccgctca
     5761 aaattcggat cacggtagat cgtcagttcg aggatacgcg caaccgacat gtaattggcc
     5821 gtgtcaaggt ttgtgtcgct gtcgacattg ccgtcccagc aaccgcagca aacataatcg
     5881 cgggcgcgcg ccacgggata tcccaaggcc atgaagttcg gaatgtgccg gtcgtcgttg
     5941 aagacggcaa acaccgcatg cccgcgcatc agcatcgcgc cgatcttctc gagatattct
     6001 tggggcgact tctctgaaat cctgcaatga agctttggga acacaaggtc gagcccgata
     6061 tgcgcgtcca ggaacgcagc cgtcaattcg ttgtagacag gacgtccctc cgcatcgcag
     6121 cccccgagcg tcagggggat ttccgcctcc tgatccgaat agccgtcgat ctccagccgt
     6181 gtatcgagat gacagtcggc catgacaaga aaacgggcga tgagatcgcg cgcctccggc
     6241 tccgttagcc gtccggcggc aagatcgcgc cgatagaagt cgatcagcca tgcatcggga
     6301 cgcccgaggg aaaaattgag cgttccctcg acgtacccga ggatctcccg gacgaaccaa
     6361 agggtgttca atccctcgta gaaatcgcgc ggcggctccc acgggcaccg acgagccgcg
     6421 acggcaatcc gttccagtct caaacggtct tccgcagaca ggccgacggc cgccaactgt
     6481 ctctcggcct cgcgcacaaa agcaagctgg atctcatgca ccgtatccag cccgacaagc
     6541 gccacttcaa gccccttacg cccggaggca tcttccgccg gacaggatgc cagcgcggtc
     6601 tccacttctc gacgaacgcc gcgaaagcct ttggtgaaaa tcgtatggaa cggcggaata
     6661 tgatgcatgt cgtcgacgaa atcgcccgtt aacataagac atccgcgtct cctcgcgcgc
     6721 aagcgcgcga aggcctcccg tggaatatac cgctcctcaa gccacccacg gcagattcga
     6781 ttgatgtgac gcccgggaac ggattccgcg ccctgccaga cacaccatcc gccgtttacg
     6841 cccgcttcgc agtagaaagg cgatccaggc agaatgtacg gcacgaaatg ccgccgcatc
     6901 gcaaggtatg actcgcgccg gatatccagc acatcgcaat cgggatgagc cgccacgtag
     6961 gcatccagtt cattgccaat catccggacc gacgcaactt ggcgcggatc gcggcacttg
     7021 ccgtggcact cgtccggata gaatgcgcgg agcgatttcc gaaatgtttc gtaatccgcc
     7081 gcggcaaaag gcctttcccg cgcagcggaa agcgcggcag gcgcatcaag ccccaaggcc
     7141 gcaaaaaatg ccgcggccga tcccataaat tcccgacgct tgaccttcat ctcctgttca
     7201 tcccctgcgt tcggttcgtg acagggaggc atgcgagtcg agccgttccc gactcgctgc
     7261 tccctttttc gtcaggcacg caagatcttt gcccatccct gtcagcaacc ggcatcctca
     7321 cttgaggatg atcgccaatc ccaccctgcc tgccaacagc gtgccatcac ccgtgatgag
     7381 cgcccgacgg ttttccggcg gcacggagag attggtcgcc gagccataaa aaccgtcctt
     7441 acacttcacc cagtctccgt tcttcttcag ccacaaagag ccgatttgct gcacgactcc
     7501 cgaggcgagc tccagcgtgc ccccttcgag atagaactcg gcatccttcg gaaatgcata
     7561 cgaatgaccg atcaccagct caccgccgga aaccgtcgcc ttcttgcccg cccaggtccc
     7621 ggccgtcgcg cttgtctgcg gataccgcgt ccaaacattg ttcaccttct gccagaaatc
     7681 ccattggtca ccggccgccg caaagacgag acgtccggcc acgacagaca cctcgcccgt
     7741 cgatatgttg gcccgccgga acgtatttgt gcctgcaccg gcccgttcga ccgacgccgc
     7801 cccgccgaag accggcatga tgcccgtatc ggcattcgac gttccctgac aagggattgc
     7861 gtccgtcaaa acgagcttcg acccgctcgc gccgaagatc gtgcccccgt tcgcggccga
     7921 cacgtcgcct ccacatgccg tcagaacacc gtccgtcaag acgccctcac agcagcagtt
     7981 cgcgaccgcc tgacgccaac cgccgagatc cacacgcgcc ttgggggaga cgatctgaat
     8041 cggcacggca ttcgaaaaca cattcggcgc cgaatagaca tgcgtgcagc agatcttgaa
     8101 gttataatcc gtggcgaact tgctgccgga agcccccagg acaaaccggg cgtagttgtt
     8161 gagctcgctc gaagctccgt agagaaccgc atcgttgtca ttcaggtgcg gcgaggtaaa
     8221 acgcacttca gacgcgtcat cgaccagact cggatacaac aaccgttggg tgatcagcat
     8281 gaaatcaccg gcaaagtcca gccgcgcctt cgactgaaca gtaaatgtga aaaaaccgtt
     8341 ctgcaggctg accagaccgg ccagaacgtt cgttgtgctt gccagcgcat aaagttggtt
     8401 gtgcccatcc ggattgatgc gtgacacggg acggttcaca acagcgttgc taaggtagag
     8461 agccgtctcc ttgtgctttt cggcctccgg atagatatac agcgcaccgg ttccgcccag
     8521 cggatcgagg cccgtgaggt acgtatggcc cgtgcgaacc gtaacgtcgc cataaatctt
     8581 attggcgttc ccggtaatcc gcagttcgcc ataccccgtc ttctcgaagg acgccccgcc
     8641 ggccgaatcg aacgacgatt caaacgaaag cgaactcccc ttcggcaccg aggcggcgag
     8701 atcgccaccg aatttgaaag gcgacttgaa gacaatcttc ttgacgcggt cggacgtccc
     8761 cggacggatg tcgataactc cgttggccag catcagccga ccgttcgctt ccggcgtcga
     8821 cgtgaattcg accgtcccgg acgcatcttc atcgaaaaca agcgacttgg cgttcacggg
     8881 aacatccagc tcaacggtcg cattcgacgg gaaggaaaag acattgcccg tatagccgaa
     8941 gtccggaagc tcggtggcgt tcttccagtt cccgagcgtc gtcaccttat cgtcaacacc
     9001 tccgccgcac catgtccacg cctgttccgg cggatcgtcc ggcggagcga cgaagatcac
     9061 gcccgcgcca gaaaggcctt cgcaggcctt tatctccccc gaggcagtgg cggcatacca
     9121 cccgaatgcc agagacgtcc ccgtggcatc ccgcaactcc ttgaccgaat aggaaccctc
     9181 ggcgagttca agcgtgccgc cttccagaaa aatcctggcc acgccattgg agaaagcgtc
     9241 cgccgagcag gtcttgagga cggcaccgtt tccaacgcgc accgtgccga cccactgcgc
     9301 cccgttcgtc aagaccgtcg ttcccgacgt cgcgatcagc tcgccggaag aagtgctttt
     9361 cgccagcagt gtgccggtga tacccgacgc cagcttcatc tcaagcgacg ctgtaccttc
     9421 gaaaacattc cggttgatga aatccacacc gtcatttacg gtaatgagac cgccgacgga
     9481 gctcgtcaca cgacccagcc gcgtcacgtc attccacttg tcccaggagg gatccgtgta
     9541 ggtcgtcaga cggcgaacgc tctgcgtcgt gccattgaga tccaatttac tgtatcccgg
     9601 gtaaaaagta ttatcagtgg cttcgaaaca caaagcggaa ctctccgtaa tcgccccgtt
     9661 cacgtcacag cggagggtga aggcaatacg cgaccaggga taccccagcg gcgcatacgg
     9721 tccagacgtg catctgttca ccgtattgct gacactcgcc agaatcacct tccccttgtg
     9781 tgtatacacg tacggcagga ggacggacgg gttgttggta aagacaagtt cggccccttc
     9841 cgcgaaagaa aacagcaaga gcgaatccaa attcgacgcg cccgtgacaa agagcttgcc
     9901 attgatcgtc ccgcggaaat tgcccttttt gacgatcgtc cccaccgtcg tgactccgtc
     9961 gaacgtcacc gtcttctgat cgactagagt aaattccagt gtgcgaagag aacgaatgcc
    10021 tttataataa agttgagtcc ggctaactgc ggtttggtcc ggattgacca taatccggcc
    10081 cgcatccgcg gcgccgaagg cattgtccac cgtcccatag acaaatccat gcagaacctc
    10141 gagattaccc gtgaaagcgt tcgtggcgtt gaaatagacg ctgccgtagt tcgatgcgct
    10201 acccttttcg ccaacgacaa gagggccggt tccggaaatg acgccattga agtcgacccg
    10261 cttattcgcc gcgacgcgga tcgtcacctt ccccggcaaa gaaaaagcac ttgatatcac
    10321 gacatcgccg gtgaagtcgg ccagactgtc cgcaaccgga acgcctccgt cccagttgct
    10381 ggagtcattc caatcgcctg ccgctccgcc gcccgtccag gttgaaccac agacagaaaa
    10441 cgtcaccgcc actgataacg cacagatcca ttttccaagc atattcagtt gcatttttct
    10501 gctccttctt ttcctaccag aatagaccgc gaaaacaacg tacgaaaagc ctctaaaaat
    10561 ctcaattgaa aaatatgcaa ctatcgctta aatatttgca acacgggtag tcggtcagtg
    10621 acaggggtgg agtgtgcgaa gatcatgcgt ttccggtgaa aaggagagca aagagtcggg
    10681 agcgcgggcg tccccgcccg caacagcgtg ccttgcgaaa ggctgatgcg catacttccc
    10741 ttgggaacca aaccgcgcat cgggatatca atcggccttt cacagagcgg gcggggacgc
    10801 ccgcgctccc gactgcgcat acacaaacca cgcactaaac tcgcatgcct cctgtcacca
    10861 acccgattac tgtgcgctgg aacacgcgat tgacaaagcg acagaaaaga gaaatttgat
    10921 ctttgccgtg ctggccttgc tgggcatgca gaatccgttc gtgccaaagg ttcaaattac
    10981 ctgtccctta gttgtctctg aatttgctat gcttgcatcg ctatgacaga tagtaaggcc
    11041 aagagagtcg gggtccttgt tgaactgacc cgcgagttcg ggcgcgacct ctgcaagggg
    11101 attaccgatt tcgcttgttc ccatgaaggc tttatcccct ttttcatcac cccggagacg
    11161 cttaaacgca aatccgaact caaatcgtat gacggattca tcgcccgcgt catgtccgat
    11221 gagatggcgg cgacccttgc cgcacttaag aagccgatcg tcgacatgta ctatgacaag
    11281 ccgcgcccgg ggttcgccat cgtcaagacc aaccactccc gcatcgggcg gctcgcggca
    11341 gagcacttcc tagagcgaaa attcaagaat ttcgccttct gcggctttgc cggcggacgt
    11401 ttttcgaact actgttcccg tgccttccag cgggcgctgg ccgcaaaagg gcaccgctgc
    11461 cacagttatc aaccggacgc cagagccaag tacgaattcg acagcacggt tctgatcaac
    11521 gagcaactca atcgcgcacc ggatgcgaag gcgctggcga agtggatcgg gctattgccg
    11581 aaaccggtgg cggtgttctg tccgaatgac cttcgtgcct ggcagttgct gcaggtttgc
    11641 aaagaatgcg atatccatgt ccctcatgac attgcgatcc tcggactcga caacgacgtc
    11701 atcgtgtgcg gtttttccaa ccccatgata tcgagcatcg acccagacac accagcgatc
    11761 gggcgcgagg ccgcgcggac attggtcgag atgatgaatg acccggactt ccgcaaaaag
    11821 accctgatcc ggcaagtgaa ccccaaccga gtcatcggac gcgcctccac ggaagtttat
    11881 cccattgatc caccttggct ttccgatgcc ctcgtttaca tgcgtaaacg cgttagcgat
    11941 caactcaccg ccgatgatgt tttcaagcat ctcggtctct cgcacacgac cgtgaacagg
    12001 actttccgga aagtactaaa cacaaccgtg cagaaggaaa tcgccg
//
LOCUS       DAOSRB010000079        12004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5291_length_12004_cov_6.937484, whole genome shotgun sequence.
ACCESSION   DAOSRB010000079 DAOSRB010000000
VERSION     DAOSRB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5291_length_12004_cov_6.937484"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLZ74_05810"
     CDS             complement(<1..887)
                     /locus_tag="PLZ74_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOR97872.1"
                     /translation="MRLKTTCFGCRVALVFALGTLGRVSPLWAQEARRFATDPGNGAV
                     SMRLGDRTCWTYVPQSDEGKPYFHPLTIPGTDDVLTNFRPADHVWHLGFWFSWKGING
                     KNFWEPDPKGGTTRVVAQTVTPAPDLTFRAEATLDYVADGRTLVREQRAVRVETAPNG
                     DYTIEWDSTFFAREEGAVFSCTPAKRDQKGVWAAGGYAGLMWRFADSPAFTYTYTHDA
                     GRTNVMTCGESAARMEIVATAAASGARARITFRDHPENPRYPTPWFARHSAAAYQGRG
                     YYVVGPSMLFHEPLKLAPNT"
     gene            complement(977..2233)
                     /locus_tag="PLZ74_05815"
     CDS             complement(977..2233)
                     /locus_tag="PLZ74_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOR97873.1"
                     /translation="MKRGWLGAGAVWAALMGIGYAEPNRAPVPLWDLAVSPFAATTGR
                     IERVEGRVRMDGASAFAIPNRAIPDPSCFTIEMAIRFNALEEPSALCLLDQRVSDTGF
                     ALEALKWGRVGSPLTTTVNGIAYARNSFRASSNQVWRLVFAVRNGAVAVYQNNAPGPQ
                     YLLRVTPNLAPVWVGRTPLRKDRTRIADYEILSLKVYGPDLKYYLPGESADTMKNGYV
                     GGQGWLVRLPVKTDTPLPRILYYGDSISVGYGDRFTALLKDKAACYHWCHFVGGVRSF
                     PTAAFEAAASVAPFDIIVFNNGLHSLQWTPDKVTDEQIREVVRNMIRAFRKGAPKAKL
                     VWAATTPHTAKGRPVTELGELNPIVLRINTLADQVVKEEKIERVDLYTLLKDRLDLAA
                     GDQYHWSGEAYQLIAEALRKKCEELL"
     gene            2470..3585
                     /locus_tag="PLZ74_05820"
     CDS             2470..3585
                     /locus_tag="PLZ74_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOR97874.1"
                     /translation="MRKRGILCLAVIMGLAAWVGAQPLGPERMTEVKASVRKGLGWLA
                     AQQKPGGFWSDDKMPALTALPLWAWAGSGESAFREEADRAVAYLLSKQQPDGGIYTPT
                     PGRRGGGLGNYNTSICAAALYATGRREVMPAVLKARTYIASSQHIGDDWHAGGFGYDK
                     DAGRRYSDLNNTHFSLDAMRRTEGAEEFRPAGEKRADINWGAALRYALQLQNTEGETA
                     GGFAYNTQDPKGGVSTNAAGRVMLRAYGSMSYAGLLSLIHAQLDKQDPRVRSVVAYGS
                     RFWTLEENPGQGQQGLYFYYNIISRALSAAGLDALPKHAAPGEIHWREDLVRKLIALQ
                     RPDGSWVNENNRWWENDPVLATSYSLLALEFASGLTR"
     gene            complement(3611..5794)
                     /locus_tag="PLZ74_05825"
     CDS             complement(3611..5794)
                     /locus_tag="PLZ74_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOR97875.1"
                     /translation="MTVPTQNHPGQAAEESVTGTVESIVYRNDENGYTVCHVRPHSRL
                     HSRDELVTVVGNCAAIWEGEELHAEGVWVRHKSHGPQFQARVITCITPTSVEGLRRYL
                     ASGMIKGIGPTFAKRIVDRFGEQTLDIIDRESGRLREVEGIGEGRRKLIKESWTEQRG
                     VREIMIFLQSHGIGTAKASRIYRQYGADSIAVVKRNPYRLCEDVWGIGFKSADAIALS
                     VGIPHDSELRACAGLIHTLRTEAEDGGHCFTVDADLLLHAQTMLDISVEILARALEQE
                     YGKGSLVKEENRIYLRDLYRAETRVAAKLRLLLDTPRTFKPIDAGKALAWAEGKMKIR
                     LAPLQREALCNALNTKVSIITGGPGVGKTTIIRALTEIFGARKLDVRLAAPTGRAAKR
                     MSEATGREAQTLHRLMKFNPNQNRFEFNDENPMTGDCFILDETSMIDIRLMDHFLQAL
                     PHSVTLILVGDTDQLPSVGPGNVLHDLIASGMIPFCRLDTIFRQDTSGLIVRNAHRVN
                     RGEPFETRVGESDFYFVETGEPDRIIARTLELMTQRIPRKFGFDPLTEVQVLTPMRRN
                     LLGTENLNAVIQAALNPNGPTLTRGSSGFRRGDRVMQIRNNYDKEVFNGDIGFVREVD
                     TEDRSLSVLFDGRPVAYAQSELDELVLAYACSIHKSQGSEYPAVIVLMHTQHYKLLQR
                     NLLYTAITRGKRLVLVVGSSRAVAIAIKANQVRERRTTLTARLKT"
     gene            complement(5813..6163)
                     /locus_tag="PLZ74_05830"
     CDS             complement(5813..6163)
                     /locus_tag="PLZ74_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR97876.1"
                     /translation="MITRNGEREVERRGNMRGGTGTVQIEHWFRPEAFGAKVRLCARL
                     EIPPGGSIGTHRHEAEDEIYLVLSGQGLILENGRRIPVAAGDAVLTGKGGEHGVENPG
                     DEPLILAAIILLYA"
     gene            complement(6357..7475)
                     /gene="rlmN"
                     /locus_tag="PLZ74_05835"
     CDS             complement(6357..7475)
                     /gene="rlmN"
                     /locus_tag="PLZ74_05835"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOR97877.1"
                     /translation="MSAPDRPAAWGLLPSEWKPLCRERGLPGFRADQIVTGLYQTFAQ
                     SWDEFTTLPTELREQLSRSFPIEPLEVVHVHHAADGVRKLLLRCRDGECVETVLIPSK
                     GRLTQCVSSQVGCGFRCAFCASGQSGRTRDLTAAEIVGQVMAACQLLRADPQAPADPP
                     PAASAGGRPGAKPSLPRPGNIVVMGMGEPFDNYDNVLRALRILNDQKGINIGARHITI
                     STCGVVPGIRRLAEEGIQFELSVSLHAPDDALRNRLMPVNRRWPVAEVIAACRDYFAK
                     TGRVVTFEYTLVKGLNDTPQHADALSRLLRGMPCKINLIPLSPVEGFDGERPGDRACL
                     AFLDRLLRAGFATTLRQSRGRDVDAACGQLRLRKQAAK"
     gene            complement(7472..7951)
                     /locus_tag="PLZ74_05840"
     CDS             complement(7472..7951)
                     /locus_tag="PLZ74_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97878.1"
                     /translation="MSLRFRDIAFPLLFGILTLRAAEQDEVRFKTRTYTPDRTAGHAV
                     YRSEAYTESAAKRPIGKTTEPRDTRGFFGRLFRSRTAEAGASSRPATTRPTETEPYVP
                     GSPIRVPTIQADVKAVPEKQPYVASEKKEADRPFTAQAPDRGKNPLLRPRQGIREPE"
     gene            complement(7953..8579)
                     /locus_tag="PLZ74_05845"
     CDS             complement(7953..8579)
                     /locus_tag="PLZ74_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR97879.1"
                     /translation="MGIVIDHEARKRAIIAKSIQLFSEQGYDGVTYQKIADSCAIART
                     TLYKYFRNKRHIFNTAIWEVSYLLIERFEEILSSRTSTVDRLDRVMRAVLTLLFEQRV
                     ILTVILDYVLAAQRAGQQMDRTISRHTIGLRRILHRLVVEGLRKGELRRINATLATEL
                     LYAQMEAAVLRLTVSQNADYDELSRMLHQTIQNLTVPSAGSRSKNRRA"
     gene            8789..9568
                     /locus_tag="PLZ74_05850"
     CDS             8789..9568
                     /locus_tag="PLZ74_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOR97880.1"
                     /translation="MGDTVSKELAEARWRKVRTLLRERGILRVEELAAELDVSAATIR
                     RDLAALEAAGGLRRIHGGAIAPDGRDEEPLFDDKTAMSAAEKGRIAEAALRLIRPRDI
                     VYLDGGSTILALARLLIHHTQITVVTNSLRVAQVFSSGGPRMILTGGECRLISQTFVG
                     PLTRSILGQLHLDIAFMGTIGVSAETGMTTTDPAEAYTKEQAMARAARVVLLADSSKF
                     GKTSFVRFGALSALSALISDKRMPAAQRKAFQRAGVETVLV"
     gene            9590..11887
                     /locus_tag="PLZ74_05855"
     CDS             9590..11887
                     /locus_tag="PLZ74_05855"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HOR97881.1"
                     /translation="MATNNYISTRKPGFKPQKYDLGKIPAYQYKGTLKDEIAAGNITA
                     AQAVDVLEDMLTIREFEEMIVKLRTGAYDACKGYEYRGPTHVSIGQEATAVGCCAAMA
                     YDDQITSTHRGHGDSLAKGCAAIRGMSVEELKARVPECGATRRKELIEAALEKHVYRA
                     IAELFGKEDGYCKGRGGGMHIADFKAGHLGANAIVGGGVPIATGAAMSSRYFQDNKVT
                     CCFAGDGAYANGVVLEALNWAAMGQFTDPAYTDRPFGLPIVYAIINNHYGFTGRVDGE
                     VAGVATVARRAAGFADNNMHAEVVNGMDLLACLDAMRRAADLCRKGQGPVMLEFDTYR
                     NYGHSLGDPRFEYRTKEEEAAWKELDPIATFERQLLDAKVVTPRKIEALKAKVADRNA
                     RMAAQAAWSQDPDPADVIRYMYTDTRVETVPESFANAPTVGDLPVIKRTEDSQITYKD
                     AIKEAMIEEMLRDRRVVQWGEDIAEYGGAFKVTKGLIEAFGRERIFNAPISEACICGT
                     AVGAAMTGLRPVVELMYMDFCLMASDQISNQAAKWHYMSGASIEVPLVYRASVGGGKG
                     YGGQHSQTLESVFAHIPGLYVVYPATPYDAKGMLKSAIRDNNPVLFVESQIMYNYKDV
                     VPKEEYLIPLGKADVKRAGDAVSIVTWGPMLYEALKAADQLAADGISAEVVDIRSLVP
                     LDMETILASVRKTGRCVVTSHCVGIGSFTGEIASRIMAEAFDSLDAPVLRVGAKDGIA
                     PQAYSLEDAFLPHDRDIVAAVKTLL"
BASE COUNT         2199 a         3792 c         3826 g         2187 t
ORIGIN      
        1 gtgttcgggg caagcttgag cggctcatgg aaaagcatgg agggccccac gacatagtaa
       61 ccgcggcctt gatacgccgc ggcactgtgc cgcgcgaacc agggcgtggg ataacgcgga
      121 ttctcgggat gatcgcggaa ggtgatgcgg gcgcgggcac cggacgcggc tgcggtcgcg
      181 acaatttcca tgcgcgcggc cgactcgccg caggtcatga cgtttgtccg tcccgcgtca
      241 tgggtgtagg tgtaggtgaa agccggactg tcggcgaagc gccacatcag tcccgcgtac
      301 ccgccagcgg cccacacgcc cttctgatcg cgctttgcgg gcgtgcagga gaaaaccgcg
      361 ccctcctccc gcgcgaagaa ggtgctgtcc cattcgatcg tgtagtcgcc atttggcgcg
      421 gtttccaccc gtacggcgcg ttgttcacgc accagggtcc ggccgtccgc cacgtagtcg
      481 agtgtggcct cggcgcggaa ggtcaggtcc ggtgcgggcg tgacggtttg cgcgaccacg
      541 cgcgtggtcc ctcctttggg gtcaggttcc cagaagttct tgccgttgat ccccttccag
      601 gagaaccaga aaccgagatg ccagacgtgg tccgcgggac ggaagttcgt gagcacatca
      661 tcggtgcccg gaatcgtgag cggatgaaaa tacggcttcc cctcgtcgct ttgcggcacg
      721 taagtccagc aggtccggtc gccgaggcgc atcgagaccg cgccattacc gggatcggtg
      781 gcgaaccgcc gcgcttcttg cgcccatagg ggggacaccc ttccgagagt ccccagcgcg
      841 aagaccaacg cgacacggca cccgaagcaa gttgttttca agcgcaagcc tccctccttc
      901 cggcgttatc gccattacgc accgtcaacc gttctcaacg catcacctgt tttgacggaa
      961 ccgtccgctt tttccctcac aagagttctt cacatttttt ccgcagtgcc tcggcgatca
     1021 actggtaggc ttcgccggac cagtggtact ggtcgcctgc ggcgaggtcc agccggtcct
     1081 tcagcagggt atagagatcg acccgctcga tcttctcttc cttcacgacc tgatccgcca
     1141 gcgtgttgat ccggaggaca atggggttca actcgcccag ctcagtgacc ggacgtcctt
     1201 tggcggtgtg cggtgtggtc gcggcccaaa ccagcttggc tttcggcgct cctttgcgga
     1261 acgcccggat catgttgcgc accacttccc ggatctgttc gtcggttacc ttgtccggcg
     1321 tccactgcaa cgagtgcagc ccgttgttga acacaatgat gtcgaacggc gcgaccgacg
     1381 ccgcggcctc gaacgcggca gtcgggaagg aacggacccc gccgacgaaa tggcaccaat
     1441 gatagcatgc ggccttatct ttcaggagcg cggtgaagcg gtcgccatac cctacggaga
     1501 tcgaatcccc atagtagagg atacgcggca gcggcgtgtc ggtcttgacc ggcaggcgga
     1561 cgagccagcc ctgaccgccg acatagccgt tcttcatcgt atccgccgac tcgccgggca
     1621 gataatactt caggtccgga ccgtatacct tcaacgagag gatttcatag tccgcgatac
     1681 gcgtccggtc cttccggagc ggcgtacggc ccacccacac gggcgcgagg ttcggcgtga
     1741 cgcgcagcag atattgtggt ccgggcgcgt tgttctgata gacagccacc gcgccgttgc
     1801 gcaccgcgaa aaccagccgc cacacctgat tgctggaagc gcggaagctg ttgcgcgcat
     1861 aggcgatacc gttgaccgtg gtggttagcg ggctgcccac gcgcccccat ttcaacgctt
     1921 ccagagcgaa accggtgtcc gaaacgcgct gatccagaag gcacagggcg ctcggttcct
     1981 ccagcgcgtt gaaccggatc gccatctcga tggtgaaaca ggagggatcc ggaatggcgc
     2041 ggttgggaat ggcgaacgcg ctcgcgccat ccatccgcac ccgcccctcc acgcgctcga
     2101 tccgtccggt tgtcgcggcg aacggcgaga cagccagatc ccacagcgga accggcgcac
     2161 ggttgggttc cgcatagcca atccccatca gcgcggccca taccgcgccc gccccgagcc
     2221 atcctctttt cattgcaaag gcctcatgtt gccggacacg cgttcgcgtg caccggaggt
     2281 ggataaaaga tcggggtcaa cgatactaca gcctccggca gcacacaagg ggagaaaacg
     2341 tccgacaccg caggatgccg tttacgttat gaacctgaca ggccgtgaca gcgtttgata
     2401 tattaacaac tgtacggaat cggcccggcc cggcactttt gggggagggc ggtgcgaaag
     2461 gaacggatca tgcgaaaaag gggaatcttg tgtctggcgg tgatcatggg gttggcggca
     2521 tgggttggag cgcaaccgct cggcccggag cggatgacag aggtgaaggc gtcggtccgt
     2581 aaagggctcg gttggctcgc ggcacagcag aagcccggcg gattctggtc ggatgacaag
     2641 atgccggccc tgaccgcgct tccgctctgg gcctgggccg gctctggcga gagcgcgttc
     2701 cgggaagagg ccgaccgcgc ggtggcctat ctgttgtcca aacagcagcc ggacggcggc
     2761 atctataccc ccacgccggg gcggaggggc gggggccttg gcaattacaa cacttccatc
     2821 tgtgcggcgg cgctgtatgc caccgggcgg cgcgaagtga tgcctgcggt cttgaaggcc
     2881 cgcacctaca tcgcgtcttc gcagcacatc ggtgacgact ggcatgcggg cggattcgga
     2941 tacgacaaag atgcgggacg gcggtattcc gatctgaaca acacccactt ctcccttgat
     3001 gccatgcggc gtaccgaggg ggccgaggag ttccgccccg caggcgagaa acgcgcggac
     3061 atcaactggg gcgcggccct ccgatatgcc ctgcagttgc agaacaccga aggcgagacc
     3121 gcaggcggat tcgcctacaa tacgcaggat ccgaagggcg gggtttccac caacgccgct
     3181 ggccgcgtga tgctgcgcgc gtatggcagc atgtcgtacg cggggctgct ctcgctgatc
     3241 cacgcgcaac tcgacaaaca ggatccgcgt gtgcgctcgg tcgtggcgta cggttcccgc
     3301 ttttggactc tggaggagaa ccccggccaa ggacagcagg ggttgtattt ctactacaac
     3361 atcatctccc gcgcgctcag tgccgcgggg ctcgacgcat tgccgaaaca cgctgcgccc
     3421 ggtgaaatcc actggcgcga ggatcttgtc cgcaagctga tcgccctgca gcgccccgac
     3481 ggctcttggg tgaacgagaa caaccgctgg tgggagaatg acccggtgct ggccacctcg
     3541 tactcactgc tggccctgga gttcgcctcg ggcctgacgc ggtgacgcgg cggcggtggc
     3601 tgttgcggcc tcatgtcttg aggcgcgcgg tcagggtggt ccggcgttcg cggacttggt
     3661 tcgccttgat cgcgatggcg acggcgcggg aggaaccgac caccagcacc agccttttgc
     3721 cgcgcgtgat ggcggtgtag agcaggttgc gttgcagcag cttgtagtgc tgcgtgtgca
     3781 tcagaacgat caccgccggg tactcgctgc cctgcgactt gtggatcgag caggcgtagg
     3841 cgaggaccaa ctcgtcgagt tcactttgcg cataggccac aggccgcccg tcgaacagca
     3901 cgctcagcga acgatcctcc gtgtccacct cgcggacaaa gccgatgtcg ccgttgaaca
     3961 cctctttgtc ataattgttg cggatctgca tcacacggtc gccgcgccgg aagcccgaag
     4021 acccgcgcgt cagggtcggc ccgttgggat tgagcgccgc ctggatcacc gcgttcaggt
     4081 tttcggtgcc gagcagattc ctgcgcatgg gggtcaggac ctgcacttcg gtcaggggat
     4141 cgaatccgaa cttgcggggg atccgttgcg tcatgagttc gagtgtgcgc gcgatgatcc
     4201 gatccggttc gccggtttcg acaaaataga agtcgctctc gccgacgcgg gtctcaaacg
     4261 gttcgccgcg gttgacacgg tgcgcgttac gcacgatcag cccggaggtg tcctgacgga
     4321 agatcgtgtc cagccgacag aaggggatca tgccggaggc gatcagatcg tgcaggacgt
     4381 tgccggggcc gacgctcggc aactggtcgg tatcgcccac cagaatcagc gtgacgctat
     4441 ggggcagggc ctgcaaaaag tgatccatca gacggatgtc gatcatcgag gtctcgtcca
     4501 gaataaagca gtcgcccgtc atggggttct cgtcgttgaa ctcgaaccgg ttctggttcg
     4561 ggttgaactt catcaaacgg tggagggtct gggcctcgcg gccggtggct tcggacatgc
     4621 gtttggcggc acggccggtc ggcgcggcca ggcgcacatc aagtttgcgc gccccgaaga
     4681 tttcggtgag ggcgcggatg atcgtcgtct tgccaacacc cggaccgccg gtgatgatcg
     4741 agaccttcgt gtttagggca ttgcacaaag cctcgcgctg gagcggcgcg agacggatct
     4801 tcattttgcc ttccgcccac gccagcgcct tgccggcgtc gatcggttta aaggtccgcg
     4861 gcgtatcgag aagcaggcgc agtttggcgg ccacccgtgt ttcggcccgg tagaggtcgc
     4921 gcagatagat gcggttctcc tcttttacca gtgagccctt cccgtactcc tgctccaggg
     4981 ctctggccag gatttccacc gagatgtcca gcatcgtctg ggcgtgcagc agcaggtcag
     5041 cgtccaccgt gaaacagtgc ccgccatctt cggcctctgt gcgcagagtg tggatcaggc
     5101 ccgcgcaggc acgcagctcc gagtcgtgcg ggatgcccac gctgagtgcg atggcgtccg
     5161 cgcttttgaa cccgatgccc cagacatcct cgcaaagccg gtacgggtta cgcttgacga
     5221 cggcgatgga atcagcgccg tactgccggt agatgcggga ggccttggcc gtgccgatgc
     5281 cgtgggattg caggaagatc atgatttcgc gcacaccgcg ctgttcggtc cacgactcct
     5341 taatgagctt gcgccggcct tcgccgatgc cctccacctc gcgcaggcgg ccggattcac
     5401 ggtcgatgat atccagcgtc tgctcgccga accgatccac gatgcgtttg gcgaaggtgg
     5461 ggccgatccc tttgatcatg ccgctcgcca gatagcggcg caacccttcg accgaggtgg
     5521 gcgtgataca ggtgatgacg cgcgcctgaa actgtgggcc atgggatttg tggcggaccc
     5581 acacgccttc ggcgtgcagc tcctcaccct cccaaatcgc ggcgcagttc ccgaccaccg
     5641 tcacgagttc gtcgcggctg tgcaggcgcg aatgcggccg gacatggcag accgtgtacc
     5701 cgttctcgtc attgcggtag acgatagact ccacggtacc ggtcacgctc tcttcggctg
     5761 cttgccctgg atggttctgt gtcgggacag tcatgggaaa cccaccacgg ggtcaggcgt
     5821 acagcaggat tatggcggcg aggatcagcg gctcgtcgcc ggggttctcc acaccgtgtt
     5881 cgccaccctt gccggtgagg acggcgtcgc ctgcggcaac cgggatacga cggccatttt
     5941 ccaagatcag cccttgtccg gagagcacga ggtaaatctc atcctctgcc tcgtgccggt
     6001 gtgtgccgat cgagccgccc ggcgggattt ccagacgtgc gcagagacgt accttggccc
     6061 cgaacgcttc tggtcggaac cagtgctcga tctgcacggt gccggtgccg ccgcgcatgt
     6121 tcccgcgccg ttccacctct ctctccccat tgcgtgtaat catctttact cccttttccg
     6181 cgcgtgtccg cataccccgg cctcacggga tccgcgcccc cgcatgccgc gacccggact
     6241 ataccatgaa ccgcgtgaac gggcaacggc cccatccgcc gtcaacggat cctttgcgca
     6301 aaagggtgtg cggttcttca cggggaaccc atccacgtgt cggtatcgcg cgcggatcat
     6361 ttcgccgcct gtttgcgtaa acggagctga ccgcaggcgg catccacgtc tcgtccgcga
     6421 gactggcgca gcgttgtcgc gaacccggca cggagcaacc ggtccagaaa cgcgagacag
     6481 gcgcggtccc ccggacgttc gccgtcaaac ccctcgaccg gggagagcgg gatgagattg
     6541 attttgcagg gcatgccgcg cagcaggcgg gacagggcgt cggcatgctg cggcgtatcg
     6601 ttgaggccct tgaccagcgt gtactcaaaa gtgacgacgc gcccggtctt cgcgaaataa
     6661 tcgcggcagg ccgcaatcac ctccgccacg ggccagcgtc ggttgaccgg catcaaccgg
     6721 ttgcgcagcg cgtcatccgg cgcatgcagc gaaaccgaga gttcgaactg gatcccttct
     6781 tcagccaaac gccggatacc gggcacgaca ccgcaagtac tgattgtgat atggcgcgcg
     6841 ccgatgttga tccccttctg gtcgttgaga atgcgcaggg cgcggagcac gttgtcgtag
     6901 ttatcaaagg gttcgcccat gcccatgacc acgatgttgc cgggccgcgg cagggagggc
     6961 ttggcgccgg gcctgccgcc tgcggacgcg gcgggcgggg gatcggcggg tgcctgcgga
     7021 tcggcgcgca ggagttggca ggccgccatg acctgaccca cgatctcggc ggcggtcaag
     7081 tcgcgtgtgc ggcccgactg gccgctggcg cagaaggcgc accggaagcc gcatcccacc
     7141 tgcgaggaga cgcactgcgt gagacggcct ttggagggga tgagcacggt ctccacgcac
     7201 tcgccatccc ggcaacgcaa gaggagcttg cgtacaccgt ccgccgcgtg gtggacgtgc
     7261 acgacttcca aaggctcgat agggaacgac cggctgagtt gttcacgcaa ttcggtcggc
     7321 agcgtggtga actcatccca cgactgggcg aaggtctggt aaagtccggt gacgatctgg
     7381 tccgcacgga aaccgggcag accgcgttcc cggcaaagcg gtttccattc ggaggggagc
     7441 aacccccatg cggcgggtct gtccggagcg ctcattcggg ttcccggatc ccctgccgcg
     7501 ggcgcaggag cggattcttg ccgcgatccg gcgcttgtgc cgtgaagggg cgatccgctt
     7561 ccttcttctc ggacgccacg taaggttgct tctcgggaac cgccttcacg tcggcctgga
     7621 ttgtcggtac gcggatgggg gagcccggaa catacggctc cgtctcggtg ggacgcgtcg
     7681 tcgcgggccg tgacgatgca ccggcctccg cagtgcggct tcggaagagc cgcccgaaaa
     7741 agccgcgcgt gtccctcggc tcggtcgtct tgccgatagg gcgcttcgcg gcggactccg
     7801 tatacgcctc acttcgatac accgcgtgac ctgcggtccg gtcgggtgtg tacgttcggg
     7861 ttttgaaccg cacctcatcc tgctctgcgg cgcgcaacgt caggattccg aacaggagcg
     7921 gaaacgcgat atcccgaaaa cgcaagctca tcttacgccc tcctgttctt ggatcgggaa
     7981 ccggccgacg gcacggtaag gttctggatg gtctggtgca gcatccgcga caactcgtcg
     8041 taatccgcat tctgactcac ggtcaagcgc agaacggccg cctccatttg cgcgtacagt
     8101 aactctgtgg cgagcgtggc gttgatccgg cgcaactccc ctttgcggag cccctcgacc
     8161 accagccggt gcaggatgcg gcgcagcccg atcgtatgcc gcgagatcgt ccgatccatc
     8221 tgctgccccg cccgctgcgc cgccaacacg taatccaaga tcaccgtcag aatcacgcgc
     8281 tgctcgaaaa gcagggtcaa caccgcgcgc atcacgcggt ccagccggtc caccgtactg
     8341 gtccgggagg agaggatctc ctcaaaccgt tcgatcagca gatacgagac ctcccaaatc
     8401 gcggtattga agatatgccg cttgttgcgg aagtacttgt atagcgtggt ccgggcgatt
     8461 gcgcaactgt cggcgatctt ctggtacgtc accccatcat atccctgctc ggaaaagagc
     8521 tggatcgatt tggcgataat cgcgcgtttg cgcgcctcgt gatcgatgac gatacccatg
     8581 cggacctctt gccaaccggt gcggccccgt acgaaaaccg tagcaaacgt gaccgcccgt
     8641 tacattcatt tgattgtcga ttaaacgcgt ggtgacgtca agccccgact tgacgaaaac
     8701 cgaaaatgtg tcattccgcg catccgcgct tgcacccgat gagcgttttt gattaactgt
     8761 gatcgttttc gagcaaaaac gatcaaacat gggcgacacg gtttccaaag aactggccga
     8821 ggccagatgg cgcaaggtgc gcacgttgct acgtgagcga gggatcctgc gcgtggagga
     8881 gttggccgct gagttggacg tgtcggccgc caccatccgc cgggaccttg ccgcgctgga
     8941 ggcggccggt gggctgcgtc gtatccacgg tggcgccatc gcgcccgacg ggcgggacga
     9001 ggagccgttg tttgacgaca agaccgcgat gtcggccgct gaaaaggggc ggatcgccga
     9061 agcggcgttg cgtctgatcc ggccgcgcga catcgtgtat ctcgacggtg gcagcacgat
     9121 tctggcactg gcgcgcctgc tgatccacca cacccaaatc accgttgtca ccaattctct
     9181 gcgcgtagcc caggtgttca gcagcggcgg tccgcgcatg atcctcaccg gcggcgaatg
     9241 ccgcctgatc agtcaaacct tcgtcggtcc gttgacccgg tcgatcctgg ggcaactgca
     9301 tctcgatatc gctttcatgg gcaccatcgg cgtctcggcg gagaccggca tgaccacgac
     9361 cgatcccgcc gaagcctaca ccaaagagca ggccatggcg cgggcggcgc gggtcgtgct
     9421 gttggcggac agttcgaaat tcggcaaaac gagtttcgtg cgttttggtg ccctctccgc
     9481 gctttccgcc ctgatctcag ataaacgcat gccggccgcg caacgcaagg cgttccagcg
     9541 tgccggtgtc gaaaccgtac tcgtataacc cccaacacga aggcacatca tggcaaccaa
     9601 caactacatc agtacacgga aacccggttt caaaccccag aagtacgacc tcggaaaaat
     9661 cccggcctac cagtacaaag gtacgctcaa agacgaaatc gccgccggga acatcacggc
     9721 ggcgcaggcg gtcgatgtgc tggaggacat gctgacgatc cgcgagttcg aggagatgat
     9781 cgtcaagctg cgcacgggcg cctacgacgc ctgcaaaggg tacgagtacc gcggtcccac
     9841 ccacgtttcg atcggtcagg aggctacggc cgtgggctgt tgcgcggcca tggcgtacga
     9901 tgaccagatc acctccaccc atcgcggcca cggcgattct ctcgccaaag ggtgcgcggc
     9961 catccgcggc atgagcgtcg aggagctgaa ggcgcgtgtg ccggagtgcg gcgcgacccg
    10021 gcgcaaggag ctgatcgagg ccgcgctgga aaaacacgtc taccgcgcca tcgcggagtt
    10081 gttcggcaaa gaggacggct actgcaaggg ccgcggcggc gggatgcaca tcgctgattt
    10141 caaggccggt caccttggtg ccaatgcgat tgtgggcggc ggtgtgccga tcgccacggg
    10201 cgcggccatg agttcgcgtt actttcagga taacaaggtc acctgctgtt tcgcaggcga
    10261 cggcgcctac gccaacggcg tggtgctgga ggcgctgaac tgggccgcca tgggccaatt
    10321 caccgatccc gcctacacgg accgtccctt cggtctgccg atcgtctacg ccatcatcaa
    10381 caaccactac ggcttcaccg gccgcgtgga cggcgaagtc gcgggtgtcg ccacggttgc
    10441 gcgccgcgcc gcaggtttcg cggacaacaa catgcacgcc gaagtggtca acggcatgga
    10501 tctgctcgcc tgtctggacg ccatgcggcg cgccgccgac ctctgccgca aggggcaggg
    10561 cccggtgatg ctggaattcg atacctaccg caattacgga cactctctcg gcgacccgcg
    10621 tttcgaatac cgcacgaagg aggaggaggc cgcctggaag gaactcgacc ccatcgcgac
    10681 gttcgaacgt caactgttgg acgccaaggt ggtcaccccg cgcaaaatcg aggcgctgaa
    10741 ggccaaggtc gcggaccgca acgcccgcat ggctgcccaa gcggcctggt cccaagatcc
    10801 tgatccggcg gatgtgatcc gttacatgta caccgacacc cgcgtcgaaa cggtgccgga
    10861 gtcctttgcc aacgccccga ccgtgggaga cctgccggtg atcaaacgca ccgaagacag
    10921 ccagatcacc tacaaggacg cgatcaaaga ggcgatgatc gaagagatgc tgcgcgaccg
    10981 ccgcgtggta caatggggcg aggatatcgc cgagtacggc ggggccttca aggtcaccaa
    11041 aggcctgatc gaagcctttg gccgggagcg gattttcaac gccccgatct ccgaagcctg
    11101 catctgcggc acggcggtcg gcgcggccat gaccggcctg cggccggtgg tggaactgat
    11161 gtacatggac ttctgcctca tggcctcgga ccagatctcc aatcaggcgg ccaagtggca
    11221 ctatatgtcc ggagccagca tcgaggtgcc cctcgtctac cgcgcctcgg tcggcggcgg
    11281 caagggatac ggcggccagc actcccaaac cttggagtcg gtgttcgccc acatccccgg
    11341 cctctacgtg gtctatcccg ccacgcccta tgacgccaag ggcatgctga agagcgcgat
    11401 ccgcgacaac aaccctgtcc tcttcgttga atcgcagatt atgtacaatt ataaggatgt
    11461 cgtgccgaag gaggagtatc tgatcccgct cggcaaggcg gatgtcaagc gcgcgggcga
    11521 cgcggtctcc atcgtcactt gggggccgat gctctacgag gcgctcaaag ccgccgacca
    11581 gctcgccgcc gacggcattt ccgccgaagt ggtggacatc cgttcactcg tgccgctcga
    11641 catggaaacg atcctcgctt cggtccgcaa aaccgggcgc tgcgtggtga ccagccactg
    11701 cgtcgggatc ggcagcttca ccggcgagat cgcctcgcgc atcatggccg aggcgttcga
    11761 ctcgctcgac gccccggtgc tgcgcgtcgg ggccaaggac ggcatcgcgc cgcaggcgta
    11821 ctcgctcgag gacgccttcc tgccgcacga ccgcgacatc gtggcagcgg tgaagacgct
    11881 tctctaaccg caggaaaaaa acggacctgt tttagagtcg gagggcggcg gaatttccgc
    11941 cgcccttttt ttacagttgt gccctgaaaa aacagtgttt tatccgcgcg gtggtgtggt
    12001 atcc
//
LOCUS       DAOSRB010000080        11958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5316_length_11958_cov_6.437537, whole genome shotgun sequence.
ACCESSION   DAOSRB010000080 DAOSRB010000000
VERSION     DAOSRB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5316_length_11958_cov_6.437537"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..2648)
                     /locus_tag="PLZ74_05860"
     CDS             complement(267..2648)
                     /locus_tag="PLZ74_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOR97882.1"
                     /translation="MTLSLSVQVVWRMAVKEVIASRHEKIEPEHLFEAATRGKDFTGE
                     KALEELRAQGVDVETLAAELRTGSDALEKAGLNPVKLRRAVRDKLGAGTFDWASKSDD
                     VVHRSDRTKKVFAEAEKFSAALKEPSVTVRALFAALLADDTSPVCTVIREEGADLAKI
                     RASLVPSVGGKPVAQAAKADESGDASSGSLLDRFGRDLTAEARAGKLPPVIGRRKELL
                     QVIQTLARKTKNNPLLVGEPGVGKTAIVESLAQRIAQGKDGQVLKGKRLVELNVGALV
                     AGTKYRGEFEERVKKLLEEVKATPDLIVFIDEIHMLIGAGDRKGGMDAANLLKPALAR
                     GDFTCIGATTLDEYRTHIEKDAALERRFEKIVVPEPSRDETLKILEGLCERFEKHHGV
                     TIAGGALEAAVDLAIRFDADHRLPDKAIDLIDRACAQLCIPGLSIQSDKPAVGRGEVS
                     KKDVVAVLSEKTGIPVNLIAGALQGDAKSNLTGLKERLSVRVIGQDEAIRRVCERLML
                     AQAALVERRGPLGVFLFMGPSGVGKTELARALASELFGSEQTMIRLDMSEYMEQHSVS
                     RLIGSPPGYVGHEEEGQLTGALRTTPYSIVLLDEIEKAHLRVADLFLQVFDDGRITDA
                     KGRAVDAKNAIFIMTSNLGAQPSIKHKPMGFNAVVAPDVVNAPARENDDLKKHFRPEL
                     INRIDEVIEFEQLGRQEVALIAEMRMKTLSQALQAKHGVSLLYDQAVIDRLCEEGFSA
                     EFGARELSRAVQRLIEIPVARLVATHSDLDNCRIRCVLENERIDVSIVQKSDM"
     gene            complement(2645..3430)
                     /locus_tag="PLZ74_05865"
     CDS             complement(2645..3430)
                     /locus_tag="PLZ74_05865"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOR97883.1"
                     /translation="MTGVAGVSDRGSVRAENQDAWRADESLGLVVVADGMGGGPAGAQ
                     AAAIVAERLPELVRRRMGGGKRLTTPPQTGEGTRGVLRRRAARRLLREAVLELSREIL
                     RASEDDPERKGMGATAVAAWRCGPVVYVAHQGDSRAYLFRGGVLNKLTADHSIVALLV
                     RSGEITEEEAGKHPARGQLTRFVGMPGDVYCEVQTLMIKDGDRLLLCTDGLWGVVNDD
                     EIAAVLASHAEPEKGCDELIELAKRRNAPDNVTTVVWDLGGAL"
     gene            complement(3427..4827)
                     /locus_tag="PLZ74_05870"
     CDS             complement(3427..4827)
                     /locus_tag="PLZ74_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR97884.1"
                     /translation="MSNTVSFNVTSGPMKGKAFEFTEHDTFIFGRGEECHCRLPDDGQ
                     VSRRHFIVEVNPPDARIKDFGSLNGTHVNGKKIGSREKGETPEQGQQRKSPEVDLKDG
                     DELTVGQSKLAVKIVGAVGKGPIMCSRCNKDVQGEVGGRRDGAYICLACRQSIQIDPA
                     AALLEMLGKDEAKGKAGAGGTPVPQGAEKGAPTVGGYRIGKRLGIGGFGAVYLAEREA
                     DGHPVAIKVMLSRVAVDEKARLQFAQEVKLLEKLRHGNIVSLISSGSAGGAFYFVMEY
                     CPGGSVADYAAARGGKLSAKAVLPILRQALEGLAYAHAQGVVHRDLKPANILLTGKPE
                     SPVAKISDLGLAKNFDKAGFSGMTVTGAYAGTPVFMPKEQLTNFKYVKPVTDVWSFGA
                     TAYNLLTGLTPRDFPRGCDPMEVVLRGEIIPIRKRDPQIPAPLAEVIDKAILASPKDR
                     YQNAAEMLAALGKVAL"
     gene            complement(4901..5773)
                     /locus_tag="PLZ74_05875"
     CDS             complement(4901..5773)
                     /locus_tag="PLZ74_05875"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOR97885.1"
                     /translation="MTDCAASNRQKLLNMSRATGRGFQELVQYFAMSRFLYRLSKLEA
                     ADRFILKGALLLHALEIAHARSTLDIDLLGRFSNDPEAITAVVAAAIGQTVEPDGLVF
                     QANSIQISDITGEAEYMGRRVRFTGMLGVIRIPMQIDIGFGDSPIPPPTWIETPTLLG
                     YASGRMLGYAPETTIAEKAHAIIQLGMLNSRMKDFYDIRLLSHAGTLDEKNLVAALAA
                     TFTTRGSPVPAESVVFTDAFLDDASKQTQWAAFLGKRQIQDAPQRFSEIAREVVGFLK
                     PLLARARERQNSGG"
     gene            complement(5770..6372)
                     /locus_tag="PLZ74_05880"
     CDS             complement(5770..6372)
                     /locus_tag="PLZ74_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOR97886.1"
                     /translation="MRQGKFEHLIASVQPGGTFTAAEARRAGVSPRTLERFRQAGKVV
                     RLARGVYQAAEIRDAASPDYAVIAKRVPLGVLCLLSALYHHDLTTEIPREICLAVSRA
                     ANVPRIGYPRVRIYRMSAGSFAAGIERKVVDGVELRVFSAEKTLADCFKYRNRIGLDV
                     AVEALKNYMARRGRNIERVRKMAKVCRVEKIIYPYLEALV"
     gene            complement(6573..7517)
                     /locus_tag="PLZ74_05885"
     CDS             complement(6573..7517)
                     /locus_tag="PLZ74_05885"
                     /inference="COORDINATES: protein motif:HMM:NF025612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HOR97887.1"
                     /translation="MMNRLYIIGNGFDIYHGIRSEYVDFKRYLYHRDRKLHNLVEKYI
                     PVAGDWSGLESALANIDVDSITEYASEYLVSYDADGRRDNYHEYQYEIGLIVEGLSVK
                     FKSLFTEWVRQLSVPTRNTLTVCPLNITPADKFLTFNYTSTLSDIYGVPSARVLHIHG
                     EAKSGQDLVLGHAWRPVERTEPKYDPSFEFDSPDWRVTEGEELLNRYFVRTFKDTRKV
                     LADSISFFSGLMDLHEIYVLGHSMSKVDFDYYREIAKVVNVHKVKWVVTFHGEDERAR
                     HINTLNILGIPSCAVTLCEMNDLQVGGGTLRGGAFESE"
     gene            complement(7568..8872)
                     /locus_tag="PLZ74_05890"
     CDS             complement(7568..8872)
                     /locus_tag="PLZ74_05890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOR97888.1"
                     /translation="MWRSGDLVDERYEVRQVFESGGMGIVYRVYHLLWNMDLALKQPR
                     VDPSPDANRLNAFRRECELWSNLNPHPHVVTCYYVRTINEVPSVLTEFVDGGSLSDWI
                     SSRRLYSGTEENALARILDISIQSAWGLEHAHVAGLVHQDVKPGNILVMADGTAKITD
                     FGLADLVDGNIGSGLSDSPSAKFVSVNGMTPAFCSPEQRKKAQLTLKTDIYSWAVSVL
                     EMFTGEVTWANGTVAGCVLDEIKDRAPAPDIPDIPAAVFDLLGECMSEKPERRPDGFH
                     EIADRLQNVYFDIFGETHERMRPDAELAALDACNNRALSLLDLGHPKEQAERLLQSVL
                     EIDPQHQQAAANLALIRAGHTSKDRSCFHLAKPKSGFDYYEDQRKFERLCAKTKRAIE
                     TGRNQDALRYLQQADDLGSFERHPRIQRFKREVCKSITSEQG"
     gene            complement(8901..10913)
                     /locus_tag="PLZ74_05895"
     CDS             complement(8901..10913)
                     /locus_tag="PLZ74_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97889.1"
                     /translation="MTTNNREALNGCLDQITSLSPAESLEPLGERIIDLLWSVSEARS
                     DLDALPIIISEHEVSLRLSCQNGNAQQAGQSVCAVFNKIARCTPSQVREGLRSACGVI
                     DDAFKTKTPTAIAEGLRDAVRALVDERQTGGTLPVFVEGDGIRIGRHFKVTFCRTLRV
                     PDDGKNYPLPAGLGHFPIHAVADYAQRVPREWLETGGFFLPMYQSEAMYLELGGQTWR
                     PNIAKVGVGRINAVTGKPWDERIHATEQDYVLCPQQKWLDGINAGQGFVRQFVAMPLG
                     KGYTVEEQVTDECQFGGIQIAAFAAKPGIFPEIDPQEIENAKLKPGDADLMAGILKLV
                     HPYQSVLAMQVLKYSPKEILDALGNDKRDLLKVIQKDALAQLEPLIKNKDAFSRLKNE
                     IFPSDIRYSLSAGQPRYRLAGISLDELYTEPSMGMAAGGRLKQKIIGDTFGANTWDPL
                     TKGSVFIHLVNSNVYRDITGNAPPTRPIEAVHYQSMGIPWFSYYDDRQTSLAPSRILS
                     RIKSIFTVDQQRGKAEKPETPLPVDSQKIVEIKIPSRMERVHSLLSATQAAVRNGAPK
                     SVVRLTTSILDLKDDFAPAISLRAEAHLLLGFYDEAKCDADHALKLSTDKEISMAARC
                     VRAKVALLTGAPEMALLEAKAAIRVSGNDPTLAKIIEEANAILSSR"
     gene            complement(11074..11811)
                     /locus_tag="PLZ74_05900"
     CDS             complement(11074..11811)
                     /locus_tag="PLZ74_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97890.1"
                     /translation="MPTRKDAFPTTDWGLLKNLRGNSPALREAGLNLLAGRYWRPVYG
                     FLRRSGHDDAEAKDLTQAFFAAWLENDGFAKADQDKGRLRSLMLTSLKRFVSNERRSD
                     NAQRRKPAAGLVSLDELMESASHPFDPKDEGLTPDRIFDRQWAVGVVMRVLKRLEAEC
                     ETTGKAAHYDIFLQRIIAPILHGAEEKTLADLGVQHGMTEKQTGNCLLTAKRACRRLM
                     EEEVRLYAETEAEVADEIRELFLILGQ"
BASE COUNT         2518 a         3881 c         2902 g         2657 t
ORIGIN      
        1 gcttcacttc gcggctcacc gtgctcttgt ggcgacccag ttgccgcgct atctttatac
       61 cactattaga catctgtcaa ccaccctctt tttcatcacg tcacccctat tctccaaacc
      121 ctgcgcaaaa ccgcacctgc aaacctcttt ctcccccgtt gcacatgcgt cccgtccgtg
      181 attccgcgca accatccaca ccgccgcgcc cgatgcccca gtaggaccgg acctgcgagc
      241 cggccagccc cgccgtcagc ctttcgtcac atatcgctct tctgcacaat gctgacgtca
      301 atgcgctcat tctccaatac gcaccgaatg cgacaattat ccaagtcgga gtgcgtcgcc
      361 acaagtctgg ccactgggat ctcgatgagc cgctgaactg cccggctcaa ctctctcgct
      421 ccgaactcgg cgctgaagcc ttcctcacac aagcggtcga tcacggcttg gtcatagagc
      481 agcgacaccc cgtgtttagc ctgaagcgcc tgtgacaagg tcttcatcct catctcggcg
      541 atcaaggcga cctcctgccg tccgagttgc tcaaactcga tcacctcgtc aatccggttg
      601 atcagctccg gtctgaaatg tttcttaaga tcgtcattct ccctagctgg agcatttacc
      661 acgtcaggag caaccaccgc attgaagccc atcggcttgt gtttgatcga cggctgtgcc
      721 ccgagattcg acgtcatgat gaaaatcgcg ttcttggcat ccacggctcg ccctttcgcg
      781 tccgttatgc gtccgtcgtc aaacacttgg agaaaaaggt cggccacccg tagatgagcc
      841 ttttctatct catccaaaag gacaatcgaa taaggcgtcg tccttaacgc tcctgtcaac
      901 tgtccttcct cctcgtgtcc gacatacccc ggcggcgaac cgatcagccg tgacacgctg
      961 tgctgttcca tatactcgga catgtcgagg cggatcatcg tctgttcaga cccgaacagc
     1021 tcacttgcca aagcgcgtgc cagttccgtt tttccgaccc ctgacggccc catgaaaagg
     1081 aacaccccca acggcccccg gcgttcaacc agcgccgcct gggccagcat aagccgctca
     1141 cagacgcgcc tgatcgcctc gtcctgaccg atcacacgta ctgaaagccg ctccttaagc
     1201 cccgtcaggt tggacttggc atctccctgc agcgcccctg caatcaggtt taccgggata
     1261 cccgttttct cggacaacac cgccaccaca tcctttttgg aaacctcgcc gcgacccaca
     1321 gccggcttgt cactctgaat gctcaagccg gggatgcaaa gctgggcgca ggcgcggtcg
     1381 atcaagtcaa tcgccttgtc cggcaaccga tgatccgcgt caaaacggat ggcgaggtcc
     1441 accgccgcct ctagcgctcc acccgcaatc gtcaccccat gatgtttttc gaagcgctca
     1501 cacagacctt caaggatttt gagcgtctcg tcacgactcg gctccggaac aacgatcttc
     1561 tcgaacctcc gttccagtgc cgcatctttc tcgatatggg tacggtactc gtccagcgta
     1621 gtcgccccga tgcacgtgaa gtctccccgt gcgagggcag gtttcagcag gttggccgca
     1681 tccatgccac ccttgcggtc gcccgcgccg atcagcatgt gaatttcgtc gatgaacacg
     1741 atcaggtcag gcgtcgcctt gacctcttcc agcaacttct ttacgcgctc ctcaaactcg
     1801 ccgcggtact tggtcccggc caccagcgca cccacgttca attccacaag gcgcttaccc
     1861 ttgaggactt gcccgtcctt gccctgcgcg atccgttgcg ccaacgactc gacaatcgcc
     1921 gtcttgccga ctccaggctc gccaaccagc aacggattat tcttggtttt ccgggccaac
     1981 gtctggatca cctgcaagag ctccttccgg cgtccgatca ccggaggcag tttccccgcc
     2041 ctcgcctcag ccgtgagatc cctgccgaac cggtccagca acgatcccga tgacgcatcc
     2101 ccggattcat ccgccttagc ggcctgcgct acaggttttc ctccgacgct aggcaccagc
     2161 gaagccctga tcttggccaa gtccgccccc tcttctctga tcaccgtgca cacaggcgag
     2221 gtgtcatccg caagcagggc ggcgaacagc gcgcggacag tcacggatgg ctccttcaaa
     2281 gccgccgaaa acttctccgc ctccgcaaaa acctttttcg tacggtcgga acggtgaacc
     2341 acatcatcgc tttttgacgc ccagtcaaac gtacccgcgc ccagtttgtc cctgaccgcc
     2401 cgccgcaact tgactgggtt aaggccggcc ttctcaagcg catccgatcc cgtgcgcaac
     2461 tcggcggcca aagtctcgac atccacgcct tgtgcacgca gttcttcaag cgccttctcc
     2521 cctgtaaaat ccttgccccg cgtagccgcc tcaaacagat gctccggctc gatcttctcg
     2581 tgacgcgacg cgatcacctc cttcaccgcc atgcgccaca ccacctgaac cgacaatgaa
     2641 agagtcataa tgcgcctccc aaatcccaaa ccaccgttgt cacattgtcc ggcgcgttgc
     2701 gccgcttcgc caactcgatc aactcatcgc agcccttctc tggttctgca tgacttgcca
     2761 atactgccgc gatctcgtca tcgtttacaa caccccacaa accatccgtg cagagcaaca
     2821 gcctgtcccc atccttgatc atcaacgtct gtacttcgca atacacatcc cccggcattc
     2881 ccacgaaccg cgtcagttga ccgcgtgccg gatgcttgcc cgcttcttct tctgtgatct
     2941 cgccgctacg caccagcaga gccacaatcg agtggtccgc cgtcagcttg ttcaagacac
     3001 cgccccggaa cagatacgcc cggctgtcac cctgatgcgc cacatacaca accgggccgc
     3061 accgccacgc cgcgaccgcg gtcgcgccca tgcccttacg ctccggatcg tcctcgctcg
     3121 cccgcagaat ctcccgactc aactccaaga ccgcctcgcg caacagcctc ctcgccgccc
     3181 tgcgtcgcaa cacaccacgc gttccctctc ccgtttgtgg tggcgtcgtg agacgcttcc
     3241 ccccacccat ccttcgccgc accaactccg gcaaccgctc cgccacaatc gccgccgcct
     3301 gcgccccagc cggcccgccg cccatgccgt cggcgacaac gacaagcccg agcgactcat
     3361 cggcccgcca agcgtcctga ttttcagccc gcacgcttcc ccggtcgctg acaccggcca
     3421 cgccggtcat agcgccacct tccccagcgc cgccaacatc tcagccgcat tctgatagcg
     3481 gtccttcggg ctcgccagta tcgccttgtc tatcacctcg gcgagcggtg ccggaatctg
     3541 aggatcgcgc ttgcggatcg gaatgatctc gccccgcagc accacctcca tcggatcgca
     3601 gccgcgcggg aaatcacgcg gcgtcagccc ggtcagcaga ttgtaagcgg tcgctccgaa
     3661 gctccagacg tccgtcaccg gcttcacgta cttgaaattc gtgagctgtt ccttgggcat
     3721 gaacaccggc gtgcccgcgt acgcccccgt gaccgtcatg ccgctgaacc ccgccttgtc
     3781 gaagttcttc gccagcccga gatcggatat cttggccacc ggactctccg gttttccggt
     3841 cagcaggatg ttcgccggct tcaggtcgcg gtgaaccacg ccctgcgcat gcgcgtaggc
     3901 cagcccttcc agcgcctgac gaagaatagg caaaacggcc ttcgccgaca gcttgccgcc
     3961 gcgtgcggcc gcataatccg ccacactacc gccggggcag tactccatca cgaaatagaa
     4021 ggcaccgccc gccgaaccgg acgagatcag cgacacgatg ttaccgtgcc gcagcttctc
     4081 cagcagtttc acctcctgcg cgaattgcag cctcgctttc tcatccaccg ccacccgcga
     4141 cagcatcacc ttgatcgcca ccggatgtcc gtccgcttcg cgctccgcca gatacaccgc
     4201 gccgaaaccg ccgatgccca gacgcttccc gatccggtag ccgcccaccg tcggagcccc
     4261 cttctccgca ccctgcggga cgggcgtccc gcctgctccc gccttcccct tcgcctcatc
     4321 cttccccaac atttccagca gcgccgccgc cgggtcaatc tgaatgctct ggcggcacgc
     4381 cagacagata taagcgccgt ccctgcgtcc gccgacctcg ccctgcacgt ccttgttgca
     4441 ccgggaacac atgatcggtc ctttacccac tgccccaacg atcttcacgg ccagtttcga
     4501 ctgcccgacc gtcaactcat cgccgtcctt aagatcaacc tcgggcgatt tccgctgctg
     4561 tccctgctcc ggtgtctcgc ccttctcacg gcttccgatc ttcttgccgt tcacatgcgt
     4621 gccattcaaa ctcccgaagt ccttgatccg cgcatccggc ggatttacct ccacgatgaa
     4681 atgccgccgc gaaacctgcc cgtcatccgg caggcggcaa tggcactcct ccccccgtcc
     4741 aaagatgaac gtgtcatgct cggtaaactc gaacgcttta cccttcatcg gcccggatgt
     4801 gacattgaaa ctgactgtgt tgctcataac tcctccaccc cctatctaca cgaaaaatcg
     4861 gcagatcctg cgcgacgcgc cgacgacaac agaaacatcc ctaccctcct gagttttgtc
     4921 tctcgcgtgc ccgagccaga aggggcttta gaaatccgac cacctcacgg gcaatttccg
     4981 agaaacgttg cggcgcgtct tggatttgtc gcttgcctag gaaagccgcc cactgtgtct
     5041 gttttgaggc gtcgtcaaga aaggcgtcgg taaatacaac cgattcggct ggcacgggtg
     5101 acccgcgcgt ggtgaacgtc gcggccaggg ccgcaacgag gttcttttca tcgagcgtgc
     5161 ccgcgtgtga caggagccgg atatcgtaga aatccttcat ccggctgtta agcatcccga
     5221 gttggatgat ggcgtgagct ttctcggcaa tggtcgtttc tggcgcgtaa ccaagcatcc
     5281 tgcccgatgc gtaaccgagc aacgtggggg tttcgatcca agtcggaggc ggaatgggcg
     5341 agtcaccgaa gccgatatcg atctgcatgg gaatccggat gacgccaagc atcccggtga
     5401 accggacccg cctgcccatg tattcggcct ctcccgtgat atcggaaatc tggatagagt
     5461 ttgcctgaaa cacgagcccg tccggctcca cagtttgacc gatggcagcg gcaaccacag
     5521 ccgtaatcgc ttctggatca ttggagaacc gccccagcag gtcaatgtcc agcgttgagc
     5581 gggcgtgtgc gatctccagc gcatgaagca gaagggcgcc tttgagaatg aaacggtccg
     5641 cagcctccaa cttggaaagg cgataaagga aacgcgacat cgcgaaatac tgcaccagct
     5701 cctgaaaacc ccggccggtc gcgcgggaca tgttcagaag cttttgccga ttggaagctg
     5761 cgcagtccgt catacgaggg cctccagata aggataaatg atcttctcga cgcggcagac
     5821 cttggccatc ttccgaaccc gctcgatgtt acgcccgcgc cgggccatat agttcttcag
     5881 cgcttcgacg gcgacgtcca gtccgatccg gttccggtat ttgaaacagt ccgcgagggt
     5941 cttttctgcg gaaaacaccc gcagctcaac gccgtcgacc accttgcgct cgatgcccgc
     6001 agcgaaagag cccgccgaca tgcggtaaat gcgtacgcgc gggtagccga tgcggggaac
     6061 gttggcagcg cggctgacgg ccagacagat ctcgcgcggg atctccgtcg tgagatcatg
     6121 gtggtagagc gccgaaagca ggcaaaggac acccagagga acgcgcttcg cgatgaccgc
     6181 gtagtcgggc gaagccgcgt cgcgaatctc cgccgcctga taaacccccc gcgccagacg
     6241 cacgaccttc ccggcctgcc gaaaccgctc cagcgtgcgc ggcgagacgc ccgcgcgcct
     6301 ggcctcggcc gccgtgaacg tcccgccggg ctggactgac gcgatcaaat gttcaaactt
     6361 accttgccgc ataaacgctc cagaaacaat taagtggagt ggattatgcg cttctttgga
     6421 acaaatgtca aaccgcgaaa tcatgctatg tgctgcccgc caccgaacaa aactcccgcc
     6481 gtcactctga aaccgattcg tatcagttgt cttgcccgcc catttctgcc aagcacaaat
     6541 agcacataaa ttatagcttt tgtgcaatac gtctattcac tctcaaatgc tccgccccga
     6601 agtgtgccac ctcccacttg taggtcattc atctcgcaca atgtgactgc gcaacttgga
     6661 atcccgagga tattgagcgt attgatgtgt cttgcacgtt cgtcttcgcc atgaaatgtc
     6721 accacccact tgaccttgtg tacattgaca actttcgcta tttcacggta ataatcgaaa
     6781 tccacctttg acattgagtg ccccaagaca taaatctcat gcaaatccat aagaccggaa
     6841 aaaaaactga tactgtcggc gaggactttt ctggtgtctt taaaagtccg gacgaaatag
     6901 cggtttagta attcttctcc ctccgtaacc cgccagtccg gcgaatcgaa ctcaaaagag
     6961 ggatcatatt tcggctccgt cctttcgact ggtctccacg catgtccaag cactaagtcc
     7021 tgccctgatt tagcctcccc gtggatatga agaactctgg cgcttggcac accatagatg
     7081 tcggacaaag tcgatgtgta gttgaatgtc aggaacttat cggctggcgt aatgttcaga
     7141 gggcacacag tcagtgtatt acgagtcgga acagacaact gtcgtaccca ctctgtaaaa
     7201 agcgacttga atttgacaga caagccttcg acaataagcc caatctcata ttgatattcg
     7261 tgataattat cccgcctgcc atccgcatca tatgagacca aatactccga tgcatactcc
     7321 gtaatagaat ctacgtcgat attcgcaaga gctgactcaa gacctgacca atcaccagca
     7381 acagggatgt acttttcaac gagattatgc agtttcctgt cgcgatgata caagtacctc
     7441 ttgaaatcaa cgtattctga tcgaataccg tgatagatgt cgaagccgtt tccgataatg
     7501 tagagtcgat tcatcattgc ctagactcca tgcctctgcc tattaaatgc taatctagtg
     7561 cattttctca accttgctcc gaagttatgc tcttacaaac ctctcgcttg aatctttgaa
     7621 tacgcgggtg tcgttcaaag ctccccaagt cgtcagcctg ttgaagatac cgtagcgcat
     7681 cctgatttcg acccgtttcg atagcccgct tcgttttcgc gcaaaggcgc tcgaattttc
     7741 gttgatcttc gtaataatcg aaaccgcttt tcggttttgc cagatggaag caagatcggt
     7801 ctttgctcgt atgtccggcc cggatgagcg caagattggc tgcggcttgc tgatgctggg
     7861 gatctatttc caagaccgac tgtagcaggc gctcggcttg ttccttgggg tgcccaaggt
     7921 cgagcaaaga aagcgcacgg ttgttacacg cgtccaacgc ggccagttcc gcatcggggc
     7981 gcatgcgctc atgggtctcg ccaaaaatat cgaaataaac gttctgcaac cggtcggcaa
     8041 tttcatggaa accgtcaggg cgccgctctg gcttctccga catacattca ccgagaaggt
     8101 caaacactgc agcggggatg tcggggatgt caggtgccgg tgccctgtct ttgatttcat
     8161 ccaacacgca tcccgcaacc gtaccgttcg cccaagtcac ttcacccgtg aacatctcga
     8221 gaacggacac ggcccaactg taaatgtccg tctttaacgt tagttgcgcc ttcttcctct
     8281 gttcgggtga acagaatgca ggcgtcattc cgtttacgct gacaaacttg gccgacggcg
     8341 agtcggaaag gcctgagccg atgttgccgt caaccagatc tgctaggcca aagtcggtga
     8401 tttttgctgt gccgtccgcc atgaccagaa tgttgcccgg ttttacgtcc tgatgaacca
     8461 aaccagcgac atgagcatgc tccaaccccc atgccgactg gatagagatg tccagaattc
     8521 gcgctaatgc gttttcctcg gttccagaat aaagcctacg ggaagaaatc cagtcggaaa
     8581 ggcttccgcc atcaacgaac tccgtcagga cgctggggac ttcgttgatc gtgcgcacgt
     8641 agtagcaggt gaccacgtga gggtgagggt taagattgga ccacagctca cactcccgac
     8701 ggaaggcgtt tagcctgttg gcatcgggcg acggatcgac acgcggttgc ttaagagcca
     8761 agtccatgtt ccaaaggagg tgatagacgc ggtaaacgat gcccattccg ccggactcga
     8821 atacttgacg aacttcatat cgctcgtcga caagatcgcc tgagcgccac atcgttataa
     8881 acctccatac agaatcagaa ctatctggaa ctcagaatgg cgtttgcttc ttcaatgatt
     8941 ttggccagcg tcggatcgtt gccagatacg cgtatggccg cttttgcctc aagcaaagcc
     9001 atttccggtg ctccagtcaa caaagcgact ttcgcccgga cgcagcgtgc tgccatacta
     9061 atttctttgt ccgtgctgag tttgagagcg tgatcggcat cgcattttgc ctcatcgtag
     9121 aaacccaaca atagatgcgc ttccgcccgc aaacttattg caggtgcgaa gtcatcttta
     9181 agatcaagaa tcgaggttgt cagccgcaca acgcttttgg gtgcgccgtt ccttactgcc
     9241 gcttgtgttg ctgataagag tgaatggact ctctccatgc gtgaagggat cttaatttcg
     9301 acaatcttct gtgaatcgac aggaagtggc gtctccggct tctcagcctt tcctcgctgt
     9361 tgatccaccg tgaaaattga tttgatgcgc gacagaattc gcgaaggagc gagtgacgtc
     9421 tggcgatcat cgtagtagct gaaccaagga atgcccatac tctgataatg gacagcctca
     9481 atcggccggg tcggcggcgc attgcctgtg atgtccctat aaacgttact gttgacgaga
     9541 tggataaata cagatccctt ggtcagcgga tcccatgtat tcgcgccgaa tgtgtcgcca
     9601 attatctttt gttttagacg ccctccagcc gccataccca tcgaaggttc cgtataaagc
     9661 tcatctaaag atataccagc caacctatac cttggttgcc ctgcagatag actatacctt
     9721 atatcagaag gaaatatttc gttctttaag cgagaaaaag catccttatt tttaattaga
     9781 ggctccagtt gagccaacgc atccttttga attactttga gcaaatcgcg tttgtcattg
     9841 cctagtgcat ccaaaatttc tttcggcgaa tactttagaa cctgcatagc aagaacactt
     9901 tgatagggat gcacaagttt caatatcccg gccattaaat cggcatcgcc tggttttaat
     9961 tttgcatttt cgatctcttg agggtcaatc tccgggaaga ttcctggctt ggctgcgaat
    10021 gcggcgatct gtatcccgcc gaactggcac tcgtcggtca cctgttcttc aaccgtgtat
    10081 cccttgccaa gcggcatggc gacaaactgc cgtacaaaac cttgaccggc gttgatgcca
    10141 tccagccatt tctgttgcgg acaaagcaca taatcctgtt ctgtcgcgtg tatacgctca
    10201 tcccacggct tccccgtgac tgcgttaatg cggccaacac caactttggc gatattgggt
    10261 ctccatgtct gccctcccaa ttccagatac atcgcctcgc tctgatacat cggaagaaag
    10321 aatcctcctg tttcgagcca ttcgcgaggc acccgttggg catagtcggc caccgcgtgg
    10381 atcggaaagt gccccagtcc ggcgggcagg ggataatttt ttccgtcgtc gggaacgcgc
    10441 agtgttctgc agaaggtgac tttaaagtgg cgccctatgc ggatgccatc gccttccaca
    10501 aaaacgggga gcgttccgcc tgtttgcctt tcgtccacga gtgccctgac ggcatcgcgg
    10561 agcccctccg caatggccgt tggcgttttt gtcttaaagg catcgtcaat cacaccgcaa
    10621 gcactacgta ggccctcgcg cacttgggat ggcgtgcagc gggcgatctt attaaacacc
    10681 gcacatacgc tctgtccggc ttgttgcgca ttcccgtttt ggcacgataa ccgcaaagat
    10741 acctcgtgct ccgagatgat gatgggcaaa gcgtcaagat ccgaccgagc ctcgctgaca
    10801 ctccataaca aatcaatgat cctttccccc aaaggttcta atgattccgc tggcgacaaa
    10861 gaggtgatct ggtcgaggca accatttaaa gcctctcggt tatttgtagt catgttcttc
    10921 ctctccgatg ttcttatgac ccaccacagt ctcgacaccc taatccatgc ctgccatgat
    10981 acgccttgcc catactccct tctacactat tttttttcga atcctgcgcg ggaaaatcaa
    11041 agcagattga catttgccta ttgccaactg tattcactgc cccaggatga gaaacagttc
    11101 ccgtatctca tccgccacct cggcctctgt ctccgcatac agccggacct cttcctccat
    11161 cagccgccga caggcccgct tcgccgtcag cagacagttg ccggtctgtt tctccgtcat
    11221 tccgtgttgg acgccgagat ccgccaacgt cttttcttcc gccccgtgaa gaattggggc
    11281 gatgatccgc tgcaagaaga tgtcgtagtg cgcggccttg cccgtggttt cacactccgc
    11341 ctccagccgt ttcagcacgc gcatcaccac gccgaccgcc cactgccggt cgaatatcct
    11401 gtcaggcgtc agtccctcat ccttcggatc gaaggggtga gacgcactct ccatgagttc
    11461 atcgagcgac acgagccctg ccgccggctt ccgccgctgc gcgttatccg accgcctttc
    11521 gttcgacacg aaccgcttga ggctggtcag catcagcgac cggagacggc ctttgtcctg
    11581 atcggccttg gcgaagccat cgttctccag ccacgccgcg aaaaaggcct gcgtcaggtc
    11641 cttggcctcg gcgtcgtcat gcccggaccg ccgcagaaaa ccgtacaccg gacgccagta
    11701 acggcccgca agaaggttaa gcccggcctc ccgcagcgcc gggctgtttc cgcgcaggtt
    11761 tttcagcaaa ccccagtcgg tcgtcggaaa agcgtcttta cgggtcggca tcgtctgttc
    11821 ctcaggcgag cgcaatgaca ttcattgttt cacctcaatt catacatgtc atgatacgct
    11881 ttggccatag gcccttccac actaattgat aagaaatcgc ttttcaggcg gcacgggcac
    11941 ccgcccgctg gaaccggt
//
LOCUS       DAOSRB010000081        11864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5374_length_11864_cov_6.427555, whole genome shotgun sequence.
ACCESSION   DAOSRB010000081 DAOSRB010000000
VERSION     DAOSRB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5374_length_11864_cov_6.427555"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLZ74_05905"
     CDS             <1..193
                     /locus_tag="PLZ74_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97891.1"
                     /translation="QTNCEDIVKECRPGVNPGLSRYDKMTGLQAEGVELPQPETGNRN
                     GQRLGNGMGLESRQNTIGN"
     gene            226..2397
                     /locus_tag="PLZ74_05910"
     CDS             226..2397
                     /locus_tag="PLZ74_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97892.1"
                     /translation="MKALVLGVMAFCVVASGWGGSVPSPAVCVRACPDGAQSTPRLWN
                     GIAPQVKAWELGKRQETLAWFEENQFGKTPVGRLPDEVIGETYVAFTNAGVRIEIHCS
                     LPAGADAAHPAPVFLFGDHRGGNAPPDFAQETYEGIPTNSITARGYAYVRWNFNDVCP
                     NVDANHSFGRWPLGIIAYAATGDRNATNVVRQANSWGTIGAWAWGNSRVMDWIETRPE
                     LDASRVAVLGHSRGGKTALWTGAQDERFAMTISNGSGCGGARLGRAKAPKAETIRQIL
                     KNFPNWFCPTFTAWIDRDAELPHDADDLIRLIAPRLVYVASGSEDAWAGPAAEKAAWD
                     AAHDVYRAYKLEERMGYHCHDGPHKLRADDWERFMDFADRQLKAPSTADSDAIERRIA
                     AACVDGSRAVTLTKNAASVDGAWHLTRAILVPDDFTLVFDGCRVELVDGTQDNLVRNA
                     GAVEGRCTPNRGIRILGKNGAVLSGGKRNHYLPRRSGDPNGWRSVGILLCDVSDYEVG
                     GFTMEETQCWAISQERCCRGHIHDIVFGSTDILFNQDGVDVRKGCHDLVIENISGMTG
                     DDVVALTAYRDPPGSPARFGMQIGGNATLGEKDDIYNVTIRNIRARSSGGHGVIRLLT
                     CDGIRMHHITVEDVVDTATGNERRPQATIRIGDKSYHRTRRCELREMHHVDVRGVKAS
                     GKVAVWIRGPLSDSSVVSVTESPGGKKYEISAPMENVRLDP"
     gene            complement(3052..4977)
                     /gene="speA"
                     /locus_tag="PLZ74_05915"
     CDS             complement(3052..4977)
                     /gene="speA"
                     /locus_tag="PLZ74_05915"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOR97893.1"
                     /translation="MPHKLLDALDGWNSVKSAELYGVSTWGQGYFNVTEDGYVAVNLK
                     NTDGTVSIRLCDIVDGLRDRGMGLPLLLRFSDLLGDRIRLLNEAFGKAIADYDYQGCY
                     RGVFPIKVNQQQQAVHDVVTFGAPYHHGLEAGSKAELIAALAYMRDPDAFLICNGYKD
                     TEFIDLALFSLKMGLQTILVLETADELSLILERAAELKIRPRIGIRVKLSSRVGGKWS
                     ESGGERSVFGLTPSQVIDMVDALREADMLDCLELLHYHLGSQVPNIREIRDSVKEASQ
                     FYVGLVKEGARMGIFDIGGGLAIDYDGSHTNFPSSANYDMSEYCADVIEGIMTACNEA
                     GVGHPTVISESGRALIGYYSVLLVNVLDAAIYEEKDAPANIPEPQPEALENMMYVSRT
                     LSLKNVQESFNDALYYREEIQKAFKHGDITLRQHAAADCLFWYILTRIRELVQNLKSV
                     PRDLQGLDTLLADVYYCNMSVFQSLPDAWAIDQLFPIMPIHRLNEKPTRMASLSDITC
                     DCDGNINKFIDQHDVRSVLPVHALRRGEPYMLAVFLVGAYQETLGDLHNLFGDTNAVS
                     VQVDEDGDIEYVQELAGDSVGDVLSYVEYRPERLMEQFRTLAEDAVKARRITPAERRE
                     ILAAYESGLRGYTYFEQ"
     gene            5359..6501
                     /gene="hemW"
                     /locus_tag="PLZ74_05920"
     CDS             5359..6501
                     /gene="hemW"
                     /locus_tag="PLZ74_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOR97894.1"
                     /translation="MTDPLYIHVPFCDGKCHYCGFYSVLNSTRLIEDYPRLPARELAH
                     LLDTRPAAVRTPIRTLYLGGGTPATLGESGLAELFRRLAEVTPLDSVEESTVELSPAS
                     VTPSLLNTLRRLGVNRLSIGVQCFNDAALARIGRRHDAATAIRAIRMAREAGFTNLGI
                     DLIAGLPEVTPEQWLDSLQQVLSLDLQHLSVYALTVEPGTALAYQAEKGLLLPDETAQ
                     LADLDQADTLLCRAGFTRYEISNYALPGFACQHNLAVWRGSDYIGLGPSASSRLGRDR
                     WTNERDLEGYTDALGRNASPPRMHETLTALDDATERTLFALRLKEGFDPAERAARFPV
                     LQEHILGWEKKLEALERHGITERAECRWRLTARGREVGDAVIRELL"
     gene            6663..8666
                     /gene="tkt"
                     /locus_tag="PLZ74_05925"
     CDS             6663..8666
                     /gene="tkt"
                     /locus_tag="PLZ74_05925"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOR97895.1"
                     /translation="MKVTNEALALAANTIRCLAMDGVQAARSGHPGAPMGLADIAAVL
                     WLKYLKHDPADSQWADRDRFVLSGGHGSMLLYSLLHLAGYALPLDEIKRFRQFGSLTP
                     GHPEYRHTDGVETTTGPLGQGIATAIGMAIAERMAAARFNVADAETVVDHRTWIFCGD
                     GDLEEGISHEACSLAGHLKLDRVVLFYDSNGITIEGKTELALSDDTKKRFQAYQWHVL
                     EIDGHDYDAIDRAIRKAMRLTGKPVMILCKTHIGKGSPNKQDSHESHGAPLGEEEVRL
                     TKRALGFPEDQFFYVPQEVYAMFQARAARMKRVHAKWTRTFKAWAKAHPERAELWTAM
                     REDRLPADIEERLPKFDPAKALATRSASGAVLNALAPLLPQLVGGSADLAPSNMTWLK
                     EMGEIKPGDFAGRNFHFGIRELAMAAILNGVQIHGGFRIFGGTFFVFSDYCRPAMRLA
                     ALMELPVIYVYTHDSFYVGEDGPTHQPVEQIAALRCMPNITVIRPADPTETAAAWVAA
                     LKNRKGPTALLLTRQNMRVIDRSRYPAASNLEKGAYTLWQSGDGTPDLILIATGSEVE
                     LALDAAQQITDRNVRVVSMPSWELFEKQNRTYRDRILDPDCKRRLVVEAGSAFGWERY
                     IGTQGARVTLDTFGASGAYKDLARHFGFTVENVLSVARELLAR"
     gene            complement(8588..9856)
                     /locus_tag="PLZ74_05930"
     CDS             complement(8588..9856)
                     /locus_tag="PLZ74_05930"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOR97896.1"
                     /translation="MPEYLHFGAGNIGRALAGPLFSRAGYRVVFVDAVPSIVAALQQR
                     KRYRVVVKDTIPAGESETFEVCGVDGISVEDAEAVRQAVARADLIGTAVGAAHLPDVL
                     TAIASGLPLRNGRPVSILFCENLNGLSVMAREVLTRALPPGFLMDRVGLVETSIGKMV
                     PLMPAEVRERDPLEVWGEAYNVIIADRNGFAGPVPEGVEGLELKSCFQAYVERKLYIH
                     NLGHATCACHGFLRGHTRICEAMADAAVVAETRQVMQVSARALIRRYPSEFSEANQTA
                     HVEDLLRRFGNRALGDTVFRVGRDLPRKLAPGDRFIGGLRLAQAEGEDPAPVCRAIAA
                     ALHFAAVDETGHLFPADAAFRERLAREGAEALLSAHSGLDPIRDREALNLILGELKPE
                     VNHPAQRANSSRATDNTFSTVNPKWRARSL"
     gene            complement(9886..11586)
                     /locus_tag="PLZ74_05935"
     CDS             complement(9886..11586)
                     /locus_tag="PLZ74_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOR97897.1"
                     /translation="MKQILILCLAALVVALPFIFRPKPEEGGWLGDDPELVLITPHNE
                     ALRREFEVGFSHWHRLRYGRPVRFDWRVIGGTTEIMRYLTAEYEASLKRFQKSVSQRE
                     GLKRETGELAACGDDPAAVTCGIDLFFGGGVYDHAKAERQGLTVPAWPSGAEPAGLFK
                     DNDGRILIPREMNGEVWRGKAYYGSVLSAFGICYNADRLRDLGIRQPPAAWEDLADPR
                     YVGTLGLADPTKSGSVAKAFEMILHAQCVRSVAAAGFTREQIRQYEAAWATSGSAQDA
                     KPPEGVPAAYPEAIGCGWREGVNLVRRLGANARYFTDASGKVPSDVAMGAAAAGIVID
                     FYGRLQEELSARPDGRPVMTYVTPVGGSSMTADPISLLRGAPHRMLAVRFIEYLLGES
                     GQRLWNYRVGTPGGPKRFALRRLPIRRDFYPSSDPVLQAAFEQHRPFLADALWRPDAD
                     AYRLSEAFPYEARWTASHFGVQRDLIRAMCIDSGEELRRAWRAILRQGGPERQPEAMR
                     LLEALPEHPFPLSWATAVKGYSAYPRLDLLRQWTAFFREQYRQAEKVANSAADAYGGR
                     "
     gene            11585..11863
                     /locus_tag="PLZ74_05940"
     CDS             11585..11863
                     /locus_tag="PLZ74_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97898.1"
                     /translation="MKKYKMNGTVTRQNNDTETLSNQAEIGSHRKYSRKQPTAEKILT
                     FRVEMATLFEWLSLHLRAGRYAAFVIGDSSACFVCALSGAQASPPAES"
BASE COUNT         2353 a         3759 c         3485 g         2267 t
ORIGIN      
        1 ccagacaaat tgtgaggata ttgtgaagga atgccgtccc ggtgtcaatc cgggactgtc
       61 gcgatatgac aaaatgaccg ggttacaggc agagggggtg gagttacccc aacccgaaac
      121 tggaaaccgc aatggccaac ggctcggaaa tggaatgggc ttggaatcgc gacaaaacac
      181 gattgggaat tgacgcggcc ttttctattc cggataatgg ttgaaatgaa agcacttgtt
      241 ttgggagtca tggcattctg tgtggtcgct tccggctggg gcggatcggt tccgtcacct
      301 gcggtgtgcg tgagggcatg tcccgatggg gcgcagtcca cgccgcgcct gtggaacggc
      361 attgcaccgc aagtgaaggc gtgggaactc ggaaagcgac aagagacgct ggcatggttc
      421 gaggagaacc agttcggcaa gacgccggtc ggccgcttgc cggacgaggt gattggcgaa
      481 acgtacgtcg cgttcaccaa tgcgggtgtc cgcatcgaaa tccattgcag tctgcctgcc
      541 ggagctgatg ccgcacaccc cgcaccggtt tttctcttcg gtgatcatcg gggcggcaat
      601 gcgccgccgg acttcgcgca ggagacgtac gaggggatcc cgacaaactc gatcacggct
      661 cgcggctacg cctatgtgcg gtggaacttc aatgacgtct gcccgaatgt ggatgcaaac
      721 cattcattcg gacggtggcc gcttggtatc atcgcctatg ccgcgacagg cgaccggaat
      781 gcgacgaacg tcgttcgcca ggcgaactct tggggaacga ttggcgcgtg ggcctggggc
      841 aactcgcggg tgatggactg gatcgagacc cggcccgaac tcgacgcgtc gcgtgtcgct
      901 gtgctcgggc atagccgcgg cgggaagacc gcgctctgga cgggcgcaca ggacgaacgc
      961 ttcgcgatga cgatctccaa cggttccggt tgcggcggcg cacggctcgg acgagcgaag
     1021 gctccgaagg cagagaccat ccggcagatc ctcaagaact tcccgaactg gttttgtccc
     1081 accttcactg cgtggatcga ccgggatgcc gagttaccgc atgacgcgga tgacctgatc
     1141 cgcctgatcg ccccgcgact cgtctatgtg gcgagcggga gcgaggatgc ctgggcgggg
     1201 ccggcggcgg agaaggcggc ctgggatgcg gcccacgacg tctatcgcgc gtataaactt
     1261 gaagaacgca tgggttacca ctgccacgac gggcctcaca aacttcgtgc cgacgactgg
     1321 gagcggttca tggatttcgc ggaccgtcag ctcaaggccc cgtccacggc agactcggac
     1381 gcgatcgagc gtcgcatcgc ggcggcatgc gtcgacggct cgcgcgccgt gacgttgacg
     1441 aagaatgcgg catccgtgga cggcgcttgg cacctgacgc gggcaatcct cgtgccagat
     1501 gacttcacgc ttgtctttga tggttgccgc gtggagcttg tggacgggac acaggacaat
     1561 cttgtgcgca acgcgggtgc cgtagagggg cggtgtacgc cgaaccgcgg cattcgtatt
     1621 cttggcaaga atggagcggt tttgagcggc ggaaaacgca accactatct gccccggcgc
     1681 tcgggcgatc cgaacggttg gcggtcggtc ggcattctcc tttgtgacgt gagcgactac
     1741 gaggtcggcg ggttcacgat ggaggagaca cagtgctggg cgatttccca agaacgctgt
     1801 tgccgcggtc acatacacga tatcgtcttc ggctcgacgg acatcctgtt caatcaggac
     1861 ggtgttgacg tgcgcaaggg atgccacgac cttgtgatcg agaacatctc cggcatgaca
     1921 ggtgacgatg tggtcgcgct gacggcttat cgtgatcccc ccggctctcc agcgcgcttc
     1981 ggcatgcaga tcggcggcaa tgcgacgctg ggcgaaaaag atgacatcta caacgtgacg
     2041 attcgcaata tccgggcccg ttcgtcggga gggcatggcg tcatccgtct gctgacgtgt
     2101 gatggcatcc ggatgcacca catcacggtt gaggatgtcg tcgatacggc aacggggaac
     2161 gagaggcgtc ctcaagcgac aatccgcatc ggagacaaga gttaccacag gacaagacgg
     2221 tgcgagttga gagaaatgca ccatgtggat gtccggggcg taaaagcttc ggggaaggtc
     2281 gcagtctgga tcagggggcc gctgagcgac tcctcggtcg tttctgtcac cgaatcgccg
     2341 ggcggcaaga aatatgagat ttccgcgccg atggaaaacg tccgccttga cccgtagcgt
     2401 gtcaggttaa cctaaaaaga gtagttgaac cttcaactct ctttggtttc agggtttcag
     2461 ggtttcaggg tttcagggtt tcatccaaat cgggatcggg atcgctatcg ggatcgctat
     2521 cgggtttctg ggtttcaagg tttctgggtt tctggtttca aggtttcatc caaatcgggg
     2581 tcgggatcgc tatcgggatc gggatcgggt ttcaaggttt cagggtttca tccaaatcgg
     2641 ggtcggaatc gctatcggga tcgggatcgg gtttcaaggt ttcaaggttt caaggtttca
     2701 tccaaatcgg ggtcgggatc gctatcggga tcgctatcga ttgtttcacc acagaacacc
     2761 accgcctcac cgcctaacag cctcacagtc tcaccgcctc acagcctcac agtctcaccg
     2821 cctcacagtc tcacagccta acagcctcac agtctcacat cctcacagtc tcacagcctt
     2881 tgagattgcg ggctccgccc gcatctcaag gtgcggcggg gaccgcgaca aaacccaatg
     2941 tttacggggc tttgctggaa ttctacccgt tcaaccgctc tttttaggtt ttaaatgcgc
     3001 agaccggcat ggcgttcgga tacgctcgtc agagcggctt ttccccgcag atcactgttc
     3061 aaaataggtg tagccgcgta agccgctctc gtaggcggcc agaatctcgc gacgctcggc
     3121 aggcgtgatg cgccgggcct tgaccgcatc ctcggccagt gtgcggaact gctccatcag
     3181 gcgttcgggc cggtattcga cgtaactcag cacatccccc accgagtcgc ctgccagctc
     3241 ctgcacatat tcgatgtcgc cgtcctcatc cacctgaaca cttaccgcgt tggtgtcgcc
     3301 gaacagattg tgcaggtctc ccaaggtttc ctgatacgcg ccaacaagga aaaccgccaa
     3361 catgtacggt tcaccgcgcc gcaacgcgtg taccggcaag accgaccgga catcgtgctg
     3421 atcgatgaat ttgttgatgt tcccgtcgca atcgcaggtg atatcggaaa gggaggccat
     3481 acgcgtgggc ttctcgttca accggtggat cggcatgatc gggaagagct gatcgatcgc
     3541 ccacgcatcg ggcagcgact ggaacacgct catattgcag tagtagacat cagcgagcag
     3601 cgtatccagc ccctgcagat ccctgggtac gctcttcaag ttctgtacca gttcgcggat
     3661 gcgcgtgaga atgtaccaga acaggcagtc ggcggcagcg tgttgccgca gggtgatgtc
     3721 gccgtgtttg aaagcttttt gaatctcctc gcggtaatat agggcgtcgt tgaaggactc
     3781 ctgcacgttt ttgaggctca gcgtgcgcga gacgtacatc atgttctcga gcgcctccgg
     3841 ctgcggctcg gggatattcg cgggcgcatc cttctcctcg taaatcgccg cgtccagcac
     3901 gttgaccagc agaaccgagt agtagccgat cagcgcccgc ccggattcgc tgatcacggt
     3961 cgggtggccg actcccgcct cgttgcaggc ggtcatgatc ccctcgatca catcggcaca
     4021 gtattccgac atgtcgtagt tggcactgct ggggaagttg gtgtgcgagc cgtcatagtc
     4081 aatcgccaga ccgcccccga tatcgaagat ccccatgcgg gcgccctctt tgacgagacc
     4141 gacgtaaaac tgggaggcct ccttgacact gtcgcggatc tcgcgaatgt taggcacctg
     4201 cgacccgaga tgatagtgca gcagctccag gcaatcgagc atgtcggcct cgcgcagagc
     4261 gtccaccatg tcaatcacct gggaaggcgt cagaccgaac acgctgcgct cgccccccga
     4321 ttccgaccat ttgccaccca cccggctgga gagcttcacc cggatgccga tgcgcggccg
     4381 gattttaagt tctgccgcgc gctcgagaat cagactcagc tcatcggcgg tctccagcac
     4441 aaggatggtc tgaagtccca ttttgaggga gaacaacgcc agatcaataa actcggtgtc
     4501 cttgtacccg ttgcagatga ggaaggcgtc cgggtcccgc atgtaagcaa gggctgcgat
     4561 caactccgct ttggatcccg cctccaaccc gtgatggtag ggtgccccga aggtcaccac
     4621 atcgtgcacc gcctgctgtt gctgattgac cttgatcggg aatacgccgc ggtaacaccc
     4681 ctgatagtcg taatcggcaa tcgccttgcc aaacgcttcg ttgagcagac ggatacggtc
     4741 gcccagcaga tcgctgaacc gcaacagcaa cggcagtccc atgccgcgat cacgcaaccc
     4801 atcaacgata tcacaaagcc ggatactgac tgtaccgtcc gtgtttttca ggttgaccgc
     4861 cacatagccg tcttccgtga cgttaaaata gccctgcccc cacgtgctga ccccgtagag
     4921 ctcggcgctt ttgacgctgt tccagccgtc cagtgcatcc aaaagtttat gcggcatttc
     4981 gggtggttcc tcccccatca catcctttgt cggatcggac tcggacgcgc cccgtttaac
     5041 gcgccggtcc cgcacctcct aaacacggtc gcaacatgcc aaacccgcga ccaaagtgca
     5101 acataaaacg gagaaaacat ggcacggatt tgttttcccg tgagcaagga gaagcagacg
     5161 cccgtccctt gtctcccaga aaactgctcc tgacctgaaa catcggggtc gaaaacggga
     5221 aaacaacgga agcgactcgc ttcgacgcgg ttcattctgt atgctctttt ctgatgcccg
     5281 aatccttgtg ttgcgggttt tgcccgcaca ggataacgag ccttctcgtt ctcccctgtg
     5341 aaggaaaccg attgagtcgt gacggatccc ctctacatcc atgttccgtt ctgtgatggt
     5401 aaatgccact attgcggctt ctactccgtg ttgaacagta cgcgtctcat cgaagactac
     5461 ccccgcctgc ccgcccgcga gttggcgcat ctcttggaca cccgccctgc cgcagtgcgt
     5521 accccgatcc gaaccctgta cctaggaggc ggcacccccg ccacactggg cgagtctggc
     5581 ctcgccgaac tcttccggag gcttgccgag gtgacgccgc tggattccgt ggaggagagc
     5641 acggtcgagc tgagccccgc ctcggtgacc ccgtcgctcc tgaacacctt gcggcggttg
     5701 ggggtcaacc ggctgagcat cggcgtccaa tgcttcaatg acgccgcgct tgcgcggatc
     5761 ggacggcgtc acgacgccgc gactgcgatt cgcgcgatcc gcatggcgcg tgaggccggg
     5821 ttcacgaacc tcggcatcga tctgatcgcc ggtttgccgg aggtcacacc ggagcaatgg
     5881 ctggattctc tccagcaggt attgtcgctt gacctgcaac acctctcggt ctacgcccta
     5941 accgtagagc ccggcaccgc gctggcctat caggcggaga aagggcttct ccttcccgac
     6001 gaaaccgcgc aattggctga tctcgatcag gcggacaccc tcctgtgccg cgcggggttc
     6061 acgcgctatg agatctccaa ttacgcgcta ccggggtttg cgtgccagca caatctcgcg
     6121 gtctggcggg gttcggatta catcgggctg ggtccctccg cctcctcgcg cctcggccgc
     6181 gaccgatgga ccaacgagcg tgatctggaa ggatacacgg acgcgttggg acgaaacgcg
     6241 tccccccccc ggatgcacga gacactgacc gcgctggacg atgcaaccga gcgaaccctg
     6301 tttgcgctgc gtctgaagga ggggttcgat cccgccgaac gcgccgcgcg ttttcccgta
     6361 ctgcaagagc atatccttgg ttgggagaag aaactggagg cgctcgaacg gcacggcatc
     6421 accgaacggg cggaatgccg ctggcgcttg accgcacgcg gtcgggaggt cggcgacgcc
     6481 gtgatccgcg aattgctgta gcgcgggctc atccccgttt cccttccccc tttcttcctc
     6541 actacctttt tttccctttt tcttgtcttt cgtcttgatc ttttgacatc ctgccccctt
     6601 ttatgagaca gtgaaacgta atttccgttt ccgtgaaaag tctgaagaag gagtgtcgca
     6661 tcatgaaagt gacgaatgaa gcattggccc ttgccgccaa cacgatccgc tgccttgcca
     6721 tggatggcgt ccaagcggcg cggtccggac atcccggtgc ccccatgggc ctggccgata
     6781 tcgctgccgt gctgtggctg aaatatctga agcatgatcc ggcggattcc caatgggctg
     6841 accgcgaccg gttcgtcctc tccggcgggc atggctccat gctgctctac agtctgctgc
     6901 atctggcggg atacgcgctg cccctcgacg aaatcaaacg ctttcgccag ttcggcagcc
     6961 ttaccccggg acatccggaa taccgacata cggacggggt cgaaaccacc accggaccgc
     7021 tcggacaggg tattgccacc gcaatcggta tggcgatcgc agaacgcatg gccgccgccc
     7081 gcttcaatgt cgcggacgcc gaaacggtgg tcgatcaccg cacgtggatc ttctgcggcg
     7141 atggcgatct tgaggaaggt atcagccacg aggcctgttc gctggccggg catctgaagc
     7201 tggatcgggt tgtgctgttt tatgacagca acggcatcac catcgaaggg aaaacggaac
     7261 tcgccctcag cgatgacacc aaaaagaggt tccaagccta ccaatggcat gttttggaaa
     7321 tcgacggcca cgattacgac gcgatcgacc gggcgatccg taaggccatg cgcctgaccg
     7381 gaaaaccggt gatgatcctc tgcaaaaccc acatcggcaa aggcagtccc aacaagcagg
     7441 acagccatga gtcacacggc gcgccgctcg gcgaggagga ggtgcgcctc acgaaacgtg
     7501 cgctcggatt cccggaggac cagttctttt acgtgccgca ggaggtgtac gcgatgtttc
     7561 aggcgcgcgc cgcgcggatg aaacgcgttc acgccaaatg gacgcgcacc ttcaaagcct
     7621 gggccaaggc tcatccggaa cgggcggagt tgtggaccgc catgcgcgaa gaccggctgc
     7681 ctgctgacat cgaggagcgg ttgccgaaat tcgatcccgc caaagcgctc gccacacgca
     7741 gtgcttccgg tgccgtgctc aacgcgctcg cgccgttgct cccgcaactg gtcggcggct
     7801 cggctgacct cgcgcccagc aacatgacct ggctcaagga gatgggcgag atcaaacccg
     7861 gcgacttcgc gggccgcaac ttccacttcg gcatccgcga actcgccatg gccgccattc
     7921 tcaacggggt acagatccat ggcgggttcc gtattttcgg cggcacgttc ttcgtgttca
     7981 gcgattactg taggccggcc atgcgtttgg cggcgctcat ggaactgccg gtgatctatg
     8041 tatacaccca cgacagcttc tacgtgggcg aggatggtcc cacacaccag ccggtcgaac
     8101 agatcgccgc cttgcgatgc atgccgaaca tcaccgtgat ccggcctgcg gatccgaccg
     8161 aaaccgcggc cgcctgggtt gccgcgctga aaaaccgtaa aggccccacc gccctgctgc
     8221 tgacccggca gaacatgcgg gtgatcgacc gctctcgtta ccccgccgcg tcgaatctgg
     8281 aaaaaggtgc ctacacccta tggcagagcg gcgacggtac gccagacctt attctgattg
     8341 cgaccggctc agaggtcgaa ctcgcgctgg atgcggcgca gcagattacg gaccggaatg
     8401 tgcgggtggt cagcatgccc tcgtgggagc tgttcgaaaa acaaaaccgc acctaccgcg
     8461 accggatcct cgatcctgac tgcaaacgcc gtctggtcgt ggaagcaggc agtgcattcg
     8521 gatgggagcg ttatatcggc acccaaggtg cccgcgtcac cctggacacc ttcggcgcgt
     8581 ccggcgctta caaagacctg gcccgccact tcgggttcac cgtcgagaac gtgttgtccg
     8641 tggcgcgcga actgttggcc cgctgagcag ggtgattgac ttcaggtttc agctcaccga
     8701 ggatcaggtt cagcgcctca cggtcgcgga tcgggtcgag cccgctgtgc gcgctgagca
     8761 aggcttccgc accttcgcgt gccagccgtt cgcggaacgc ggcgtcagcc gggaataggt
     8821 gccccgtctc atccacggct gcgaaatgca gggctgcggc aatcgcgcgg cagacagggg
     8881 cagggtcctc cccttccgcc tgcgccaagc gcaacccgcc gataaaccgg tcgcccggcg
     8941 cgagtttgcg cggcagatcg cgtcccacgc ggaagacggt gtcgcccaac gcccggttgc
     9001 cgaagcgccg caacagatcc tccacatgcg cggtctgatt ggcttcgctg aactccgacg
     9061 ggtaccggcg aatcagggcg cgcgccgaga cctgcatcac ctgccgcgtt tccgcgacca
     9121 cagccgcatc cgccatcgct tcgcaaatcc gtgtgtgacc gcgcaaaaac ccgtggcagg
     9181 cacacgtcgc atggcccaga ttgtggatgt agagtttgcg ttccacatag gcctgaaaac
     9241 aggatttcag ttccaggccc tccaccccct ccggcaccgg gcccgcaaac ccgttgcggt
     9301 cagcgatgat gacattgtag gcctcgcccc agacctccag cggatcccgt tcccggacct
     9361 cagccggcat cagcggcacc atcttgccga tactggtctc caccagaccg acacggtcca
     9421 tcaagaatcc cggcggcagg gcgcgggtca acacctcacg cgccatgacg gacaacccgt
     9481 tcaggttctc gcagaacaga atcgaaactg gacgaccgtt acgcaatggc agtcccgatg
     9541 cgatcgcggt caacacatcc ggaagatgcg ccgcaccgac tgccgtcccg atcagatccg
     9601 cacgggccac cgcctgacgc accgcctccg cgtcctccac ggaaatcccg tccaccccgc
     9661 acacctcgaa cgtttcggac tccccagcgg gaatcgtgtc cttcaccacc acgcggtagc
     9721 gtttacgctg ctgcaacgcc gccacgattg atggcaccgc atccacaaac accacccgat
     9781 acccggcccg gctgaagaga ggacccgcca gcgcgcgccc gatgttcccg gccccgaaat
     9841 gcagatactc cggcatgtcc cgtccttcct ttccctgttc ccgtttcacc ttccgccata
     9901 cgcgtccgcc gcagaattcg caaccttttc cgcctgccgg tactgttccc ggaaaaaggc
     9961 ggtccactgc cgcagcagat ccaatcgcgg atatgcgctg taccctttga ccgccgtagc
    10021 ccacgaaagc gggaagggat gctccggcag cgcctccagc aaccgcatcg cctcaggttg
    10081 gcgttccgga ccgccctgcc gcagaatcgc ccgccaggcg cgccgcaact cctctccgga
    10141 atcaatacac atcgcacgga tcagatcacg ctgcaccccg aaatgtgaag cggtccaccg
    10201 cgcctcatag ggaaaggctt cgctcaaccg gtacgcgtcc gcatccggtc gccacagagc
    10261 gtcggccaga aagggccggt gttgttcgaa agccgcctga agcaccggat cggaggacgg
    10321 gtagaaatca cgccggatcg gcaaccgccg caatgcaaaa cgtttcggtc cgcccggtgt
    10381 ccccacacgg taattccaca aacgctgccc cgattcgccg agcaggtatt cgatgaaacg
    10441 gaccgccaac atgcggtgcg gcgcgccgcg cagcaggctg atcggatcgg cagtcatgct
    10501 cgatcccccc accggcgtta cgtacgtcat gaccggccgg ccatccggac gcgcggacaa
    10561 ctcctcctgc aggcgtccgt agaaatcgat cacgatcccc gctgcggccg cccccatggc
    10621 gacatccgac ggcacctttc cggacgcgtc ggtgaaatac cgggcattgg cgcccagacg
    10681 ccgcacgaga ttgacccctt cccgccaccc gcatccgatc gcctccgggt aggctgccgg
    10741 cacgccctcc ggcggcttcg cgtcctgtgc gcttccggac gtggcccatg cggcttcgta
    10801 ctgccggatc tgctcgcgcg tgaagccggc cgcagccacg gatcgaacgc actgcgcatg
    10861 aagaatcatt tcaaacgcct tggccacact gccgcttttc gtcggatccg ccaacccgag
    10921 ggttccgaca tagcggggat cggccagatc ctcccaagcg gcgggaggct gccggatacc
    10981 aagatcccgc agacggtcgg cgttgtagca gatcccaaac gcgctcagca cactgccgta
    11041 atacgctttg ccccgccaca cctcgccgtt catctcccgc gggatcagaa tcctgccgtc
    11101 gttatcctta aagagtccgg caggctcggc cccgcttggc caggccggca ccgtcagacc
    11161 ctgccgctcc gctttcgcgt gatcgtacac gcccccgcca aagaacagat cgataccgca
    11221 ggtgaccgcc gcagggtcgt caccgcacgc cgcgagttcg cctgtttcgc gtttaagccc
    11281 ctcgcgttgc gacacgcttt tctggaagcg cttcagcgaa gcctcatact ctgcggtgag
    11341 gtaacgcata atctccgttg tgccgccgat cacccgccaa tcgaagcgca ccggacgacc
    11401 gtagcggaga cggtgccagt gagagaagcc cacctcgaac tcacgccgca acgcctcgtt
    11461 gtgcggagtg atcagcacca attccggatc gtcccccaac catccgccct cttccggttt
    11521 cggccggaag atgaacggca acgcgaccac caacgccgcc aaacaaagaa tgaggatctg
    11581 cttcatgaag aagtacaaga tgaacggcac cgttacgcgt cagaataatg acacagagac
    11641 gttgtcgaat caagcggaga tcggcagcca tcgcaagtac agtcgaaagc aacctacggc
    11701 tgaaaagatt cttaccttca gggtagagat ggcaacgcta tttgagtggt tgtctcttca
    11761 tcttcgagcg ggacgctatg ccgcttttgt gatcggcgat tcgagcgcgt gttttgtgtg
    11821 tgcgctgtcg ggagcgcagg cgtccccgcc tgctgaaagt tgag
//
LOCUS       DAOSRB010000082        11810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5403_length_11810_cov_7.852999, whole genome shotgun sequence.
ACCESSION   DAOSRB010000082 DAOSRB010000000
VERSION     DAOSRB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5403_length_11810_cov_7.852999"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1176)
                     /locus_tag="PLZ74_05945"
     CDS             complement(118..1176)
                     /locus_tag="PLZ74_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR97899.1"
                     /translation="MNARAPHETGDAARSGGPRVVAWEVTRRCPLACRHCRAGALDRD
                     YEDELTTGECLRVLDSLAAFSRPMIIWTGGEPMCRPDLLDVVRGASGRGLRSVMAPCG
                     MCVTRETLVALKEAGVAACSFSLDGATAEEHDAFRGVNGAFGHVCRAMGVAAEIGMPF
                     QVNTTVSKLNRDSLPAIREQAIAAGAGTLDLFFLVPVGRGAGMRDLSLSPAETEQVLG
                     WAFEMDRQGPIRVRETCAPQAVRIWHARGKPGSPPAGCMGGRGFVFISHTGVLQPCGF
                     LDVPSGDLRAEGFDFRRAYERSAVFRELAQPERYGGACGQCAFRTECGGCRARAYAAT
                     GDYMAAEPGCLRSAAGSR"
     gene            complement(1173..2360)
                     /locus_tag="PLZ74_05950"
     CDS             complement(1173..2360)
                     /locus_tag="PLZ74_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR97900.1"
                     /translation="MVGISKLYLGKVEISDPLRYGRHSARLPSHLLQFSADKKPVVVW
                     NVTACCNLRCTHCYAATGGTSQELSTEEALGVIDDLAAFGCPVLLFSGGEPFIRPDIL
                     TLARHAVSKGLRVVFSTNGTLIDAKLAAEIRDIGVSYVGISLDGMEAVHDRFRACPGA
                     FRRSLAGIRFCREAQVKVGLRVTLTRDNVGEVPAIFDLMETERIPRICLYHLVYCGRG
                     AEIAGQDLPPDVRRATLDQIIDRTVRLHERGLPAEVLTVDNHCDGPYLYLRMVREKNP
                     RAEDVMALLRMNGGNSTGHGLACISWDGTVYPDQFWRNRPVGSVRERPFSEIWRQPAP
                     GSLLAQLREKKRHVTGRCVTCRFLDVCGGNFRARAEAATGELWGVDPACYLTDDEIAR
                     GGA"
     gene            2512..3618
                     /gene="rdgC"
                     /locus_tag="PLZ74_05955"
     CDS             2512..3618
                     /gene="rdgC"
                     /locus_tag="PLZ74_05955"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOR97901.1"
                     /translation="MAFDSGSVSFRLFYLQQAYDSALLDRFAQKAAPPISTLNRDPIT
                     GWVTGRHLFDRELTDESCLIGPYLHAQLLRAERKIPASLLRAHLRMEEDLERRARGTP
                     YLPRAVKTEIRQRVTDALLPTMPPTLSSIPTVVDFRNDLLLAAALSDKQIDALSSAFR
                     ETVGTLPVLLTPETAALRRRQINANDLTPVSFSPDTGLEPPHESSLGMDFLTWLWFVW
                     EKEGGVHHLPDGREFGIMLEGPVTFFREGQGAHEALLRKGTPLNSREAGAALVCGKKI
                     RRVKFIMACGDETYAATLDAEFAVRGLKLPPGEQNEPEGIFEERMLFIETFWNAWLSL
                     FDRFLDLRGNARQWAHTLDAMRQWVSRLSSLSAK"
     gene            3985..5577
                     /locus_tag="PLZ74_05960"
     CDS             3985..5577
                     /locus_tag="PLZ74_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOR97902.1"
                     /translation="MKKSVLYCVVLCCVTIQAATVGRWKLDDYPVGTVLKVGDTFHDS
                     VGGRDAVLCAVDPAQADTQYLPVVTNGFPAELAWEPKLWTTNSALLEMCDKAIWFKGG
                     GLKGYYLKIADDDGALKTSNMTFELFGRSVNKKFSDTVMSMPAAQDGSWGTTNSFRYA
                     SALATWPHTGALRTKADSPTGYTDTSFGWNATAKGDYYTDAWFHLAVRVSNGTLSFKR
                     DHQDMWSSCAWTHPDNTAPFIVGGAQSLDALSGCVTQIRISDTALSLSEMMFATPNGA
                     VHGTVLYHASFDEDAKCHGTVLDNYVNCNKDHVVAGTFLPTNAVQARYVHPGKEMPEG
                     ALVKNFGAAAENGENLFYTYFGTQHDNILRDLNDFTLECFVKIDEVFGTLAATNRLPL
                     VGLGHYSDADYTFRWGFKIAEDGRPIAVVTAPNYWEDSYWGAMSILDGQWHHLAMTVS
                     ENPDDGKVTVNFYVDHVLAASKTATKWYSKGLTTAQRFHVCRRNDFSGAIDEVRVTKG
                     ILDPQTEMLWQRNHGGTLLSVQ"
     gene            5672..8572
                     /locus_tag="PLZ74_05965"
     CDS             5672..8572
                     /locus_tag="PLZ74_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97903.1"
                     /translation="MRAKAINMVIFGLSMMMAAGQPTLSSEVKGRAAVDTRPYEFIDA
                     KRDADEITPWIDFEADTGWRAEGGTAQAELSQEQMLFGKFTLKVSYGPCAEVRVRPAE
                     PLALPVEHDWFGVWIRNAPENRGKEKGHELHLVFRKTNGEELRLPLSSRGNSKLDWPD
                     WWYVVRKYSKAELKTLAEPGLKFDGFVIKPASNAVPCVIYFDNIAFFNRDETKALEIP
                     RVPDVHIPTRPEGALPASLAEGGVNYVRREGKTTRLGYEGPDGKLEYVWTGDLDTLRV
                     SWNDGPAFRPTATGGAKTCPEKAVYAPSIVGKTLVLELVAPAGEKEISLGHVEGAKVN
                     YRTLIPMLGDGYWWANTRSRVSVVNDSKSDLFVLVFPDWYVSAASRIAAERRGDGTYD
                     RVIKYMALTDGSYNVVKERIYVTVSPELMETLPVIANPVSPWKHVTGKKAWSRYSAST
                     NRAEDIAFLTRLHRYGIRDLVINEHEGCMRDDGESFTFRDKSAPKKGGDKAWRKYADY
                     LINTLGYYYGPYNNFTDFPPVNANWRFDRVARRTVETDGPDAGGFVTAWTRCYNPKTA
                     FGYASCVYYAARLKEKYGFNTAYCDVHTSKPPWSYVDYDACVPGAGTFQNTYRAYAAI
                     LLEQKKAWGGPVYSEGDCQFFYAGLSDGNYAQPGIHPDKNPWIVDFNLKRIHPLECDF
                     GMGNPNMFNVDKLPTDSTSPRLVGEIDRFLAATLAYGNSPFLVVGMMFNPQSDTGNQY
                     PDPKRRATPELGLPYVLRSYFMVLPAAKRYTQAEAEEIRYLSGNGAWRTASAALFAGD
                     YEMRRIAVRYSDDTVVVANGDKVKRLKAEYGGRKLDLPPCGYAVWNTTHALEIEASDR
                     FGARTDYSESEEAIFLDTRDAVQTVAFEKARGKGLAVCRREGEGWEVIPVRGEIAFRV
                     PGDKVVAYDEAGREIGTAEIIREQDGFVTVRPVANAFSYRIMKGKIHDVPEV"
     gene            8553..9221
                     /locus_tag="PLZ74_05970"
     CDS             8553..9221
                     /locus_tag="PLZ74_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97904.1"
                     /translation="MMYPKYRRVSALAVVIAMISLLTSSAPAAVSNPTVIWSNPDVSL
                     QWKTVMSENLQFALDWPAGAVMATLTINRGRDESIITVTDTSATTLPVRMNLPADEQS
                     ESTLLLTLAYKDAAAAVLDTQTARLGLVRGIDTNTVRCVLDTTSKLWNVVKVSAVLPV
                     PEQTASLTIDGAAVEPLDSPGWYWWKIREQKNYELVLTDTRSEATAVTLSGRPRVGLV
                     IVYK"
     gene            9240..10535
                     /locus_tag="PLZ74_05975"
     CDS             9240..10535
                     /locus_tag="PLZ74_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOR97905.1"
                     /translation="MNTQLILGVFALAAGAAFASATVKDVKVTQNWPWNGKVNIDYTI
                     EAPAGALVDIDIVLRNGTRRIDIGWGAHDASRLYSQTAGTYRVTWDPAKTVGNTAPVW
                     FEFTAEVTVANNNDMFLVIDLEGDDGAGHYPVTFTDTPPAGGWNTDEYKTTKLVMRRI
                     PAGTFTMGSPYDEIGRANTSSRGPEYAKGPRTEALHPVTFTNFFYVGIFEMTEKQYCL
                     IEGIGGGSTVAVANYANGVITNGIDILSLRGANSKTQDVFPAYEADSFFGRLNTKFAL
                     TGKLANYVFDLPSISQAEYACRAGTTTTYYNGFNSSSLTNDVNLTPLAWYRNATWWRT
                     RTVGGKLPNAFGLYDTLGNANELCLDVVPDGTILWWQEGVPLVEPILYDPDHWPWPSV
                     VQGGDEEQIAKECRAACKNSGGSGGRNVGFRLALVARRK"
     gene            10560..11810
                     /locus_tag="PLZ74_05980"
     CDS             10560..11810
                     /locus_tag="PLZ74_05980"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOR97906.1"
                     /translation="MKFFVSILSIALAAAVSGAAEVTQQIVRQNWPWDNRVRIDYVVK
                     DATGGAHDVNVTVKHGNTSVTTGWYSGELYGVSEGAHTIWWNPMTNLTTYADAFWNDL
                     TVELTLVDASDMYMVIDCTTNASLAVSFTNAPPAGGWNQNLYKTSKLVLRKIPAGTYI
                     MGSPENELGRNTNRYREVQHQVTLTKSFYIGIFPFTQKQYTNLEVPTDYNPSPADPQK
                     PAINRGLEIWRGTNCWDNGVWPHCGEDGFLTRFTSRVTLPAKLTGYVFDLPTIAQWQY
                     ACRAGTIGAWNNGTTITNVTEDANLDLLARYKKNPKVGYHMSHPVGCFLPNAFGLYDM
                     HGNVHEMARDKAYGSQFAKWYTNAPAVDPLESGDPWPYVPACGGYWNDPAGECRSANI
                     GSAGGKNLTGSDAAAIRIALVYED"
BASE COUNT         2416 a         3579 c         3580 g         2235 t
ORIGIN      
        1 aggagcttcc caagcttctt acgtgggttc gattcccatc gcccgctcca ttttcttttg
       61 aacaaccctt tgcggcgcaa gcggaagttt aatttatagc gcagttcgtt ctgtctgcta
      121 tctgctgcct gcggctgatc gcaggcaacc gggttccgcg gccatgtaat cgccggtggc
      181 tgcgtaggcc cgggcgcggc agccgccgca ctcggtgcgg aacgcgcact gtccgcacgc
      241 gccaccgtac cgttcgggct gcgcgagttc gcggaagact gcggagcgct cgtaagcccg
      301 cctgaaatca aatccttcgg cgcgcagatc gccactgggc acatcgagaa aaccgcaagg
      361 ttgcagcaca cccgtatgag agatgaagac gaatccgcgg ccgcccatgc atccggcggg
      421 cggcgagccg ggtttgcccc gcgcgtgcca gatgcgcacc gcctgaggcg cgcaggtctc
      481 cctcacccgg atcggcccct gccggtccat ctcaaaagcc cagccgagca cctgttcggt
      541 ctctgcgggc gaaagcgaga gatcgcgcat cccggcaccc cggcccaccg gaaccagaaa
      601 aaagagatcg agcgtcccgg cacccgcggc gatggcctgc tcccggatcg cgggcagcga
      661 atcccggttg agcttcgaaa ccgtggtgtt cacctgaaag ggcatgccga tttcggcggc
      721 cacgcccatg gcgcggcaca cgtgtccgaa cgcgccgttc accccgcgaa acgcatcgtg
      781 ctcttcagcc gtcgcgccgt ccaggctgaa actgcacgcc gcaactcccg cctccttcag
      841 cgcgacgagt gtctcgcgcg taacgcacat cccgcacggt gccatgaccg accgcagtcc
      901 gcgtccggac gcgccgcgca ccacatcgag aagatccggc cggcacatcg gttcgccgcc
      961 ggtccagatg atcatggggc ggctgaaggc cgccaggctg tcaagaaccc gcaggcattc
     1021 acccgtcgtc agctcgtcct catagtcgcg gtccaacgcc ccggcgcggc agtggcggca
     1081 ggccagcgga cagcgccgcg taacctccca agcgactacg cgcggtccgc cgctacgggc
     1141 cgcgtcccct gtttcgtgtg gcgcgcgcgc gttcatgcgc cgccccgcgc gatttcgtca
     1201 tccgtgaggt agcaggccgg gtccacaccc cacaactccc cggtagcggc ttcggcgcgc
     1261 gcccggaagt tgccgccgca cacatcgagg aaacggcagg tcacgcaccg gccggtcaca
     1321 tgccgtttct tttcgcggag ctgtgccaag aggctgcccg gcgcgggctg tctccagatc
     1381 tcgctgaagg ggcgttcgcg cacactgccg accgggcggt tacgccagaa ctgatcggga
     1441 tagaccgtgc cgtcccaact gatgcaggcc agcccgtgac cggtgctgtt gccaccgttc
     1501 atgcgcagca gagccatgac gtcctcggca cgcgggtttt tctctcgaac catgcgcagg
     1561 tagagatagg ggccgtcgca gtggttgtcc accgtgagca cctctgccgg taagccccgc
     1621 tcgtgcaggc ggaccgtacg gtcgatgatc tggtccaacg tggcgcgccg cacatccggc
     1681 ggcagatcct gaccggcgat ctccgcgccg cgtccgcagt acaccagatg atagagacag
     1741 atgcggggga tgcgttcggt ctccatgaga tcgaaaatcg ccgggacttc cccgacgtta
     1801 tcgcgggtca gggtcacgcg cagtccgact ttgacctgcg cctcgcggca aaaccggatt
     1861 cccgccagcg acctccggaa cgcccccgga caggcgcgga accgatcatg gaccgcctcc
     1921 atgccgtcaa gactgatccc gacgtaagaa acaccgatat cgcggatctc ggctgcgagc
     1981 ttcgcgtcga tcagcgttcc gttggtggag aacaccacgc gcaagccctt gcttaccgca
     2041 tggcgcgcga gggtcaggat gtcggggcgg atgaacggct cgccgcccga gaaaagcagg
     2101 accgggcagc cgaacgccgc cagatcgtcg atgactccca gcgcctcttc cgtggacaac
     2161 tcctgcgacg ttcccccggt ggcggcgtaa cagtgcgtgc aacgcaggtt gcagcaggcc
     2221 gtgacgttcc agaccaccac cggttttttg tccgccgaaa attgcagcag atgactcggc
     2281 agacgcgccg aatgtctccc gtaccgcaac ggatccgaaa tctccacttt ccccagatac
     2341 agttttgaaa tgccgaccat ctgattttgt ctgtttcccg tcttcccgca gagtccatag
     2401 tgaaaaacga aagtgtagca aaaagggatc gcgccggaaa agcgagaaac ggcctgacat
     2461 ccgacagcgg agaggctatg ctgatccttt tttcatcccg gtgaggtttc tatggccttt
     2521 gacagcggca gcgtaagttt ccgtctcttc tatctccaac aggcgtacga ttcagcgttg
     2581 ctggatcggt tcgcgcaaaa agcggccccg ccgatcagta cgctcaaccg cgatccgatc
     2641 acgggatggg tcaccggacg ccacctgttt gaccgcgaac tgacggacga gtcctgcctg
     2701 atcggccctt atcttcacgc gcaactcttg cgtgcggaac ggaaaatacc cgcctcgctg
     2761 ttgcgcgccc atttgagaat ggaggaggat ctcgaacggc gcgcgcgcgg caccccctac
     2821 ctgccccggg cagtcaaaac cgagatccgc caacgcgtca ccgatgcgtt gttgccgact
     2881 atgccgccca ctcttagcag catcccgacg gtggtggatt tccgtaacga cctgttgttg
     2941 gcagccgcgc tctccgacaa acagatcgac gccctctcat ccgctttccg tgagaccgtc
     3001 ggcaccctgc ccgtcctgct caccccggag accgccgccc tgcggcgccg acagatcaac
     3061 gcgaacgatc tcacgcccgt gagtttctcg cccgacacgg gactcgagcc gccgcacgag
     3121 tcttcgttgg gcatggactt tctgacctgg ctttggttcg tctgggaaaa ggagggcgga
     3181 gtgcatcatc tgcccgacgg ccgcgagttc ggcatcatgt tggagggtcc cgttaccttt
     3241 ttccgtgagg ggcagggtgc gcacgaggcc ctgttgcgta agggcacgcc gctcaacagc
     3301 cgcgaggcgg gcgcggcgct cgtgtgcggg aaaaagatcc gtcgcgtcaa attcatcatg
     3361 gcctgcggcg atgagacgta cgcggccacc ctggacgccg aattcgcggt gcgcggcctc
     3421 aaactgcccc cgggtgaaca gaacgaaccc gaagggatct ttgaggaacg catgctgttc
     3481 atcgaaacct tctggaacgc gtggctttcg ctctttgacc gttttctcga cctgcggggg
     3541 aatgcgcggc agtgggcgca caccctcgac gccatgcgcc aatgggtctc ccggctgagt
     3601 tcgctgtccg caaagtaagg cgaaccctgt cccgtgagga atgagccttg ccggccgctc
     3661 cagccgcatc gtgccccgga tgacgttgtc ggatgaaccg gatgaaacag gcgacttgtt
     3721 ccgatctgac ctcctactac cgtggaacgt gccgatagac gcggcgcgtg ggaatcgctc
     3781 ttcctcgttg ccgaatacgc ctggaacgtc aaggcgccaa gcgcggagcg tatgacggcg
     3841 gcacatatta cgatccgctc gtagaccaca ccggaccgtc ggaaatttcc tccggcgggt
     3901 tactggcgga tttgcattga cgcgagagga cgtggtatac ttgtcattac aaatgcgggt
     3961 tgtaaccttg tcgaggaggt tatcgtgaag aaatctgtat tgtattgtgt tgtgttgtgc
     4021 tgtgtgacga tacaggctgc aacggtcggg cgctggaagc tcgacgacta tccggtcgga
     4081 acggtgttga aagtcggcga tacgttccac gattcggtcg gcggccgcga cgccgtgctg
     4141 tgcgcggtcg atcctgcgca ggccgatacg cagtatctgc ccgtggtgac gaacggcttc
     4201 ccggcagaac tcgcatggga accgaagctg tggaccacca actccgcgct gcttgagatg
     4261 tgcgacaagg cgatctggtt caagggtggc ggattgaaag gctactatct caagatcgcc
     4321 gatgacgacg gcgccctcaa gacatcgaac atgacgttcg aactctttgg cagaagcgtg
     4381 aacaagaaat tttccgatac ggtcatgtcc atgccggccg cacaggacgg aagttgggga
     4441 acgacgaact ccttccgata tgccagcgcg ctcgcgacct ggccgcatac cggtgccctt
     4501 cggacgaaag cggattcgcc gacgggatat acggacacga gcttcggctg gaatgcgacg
     4561 gccaagggtg attattatac ggatgcgtgg ttccacctcg ccgtccgggt gtcgaatgga
     4621 acgctttcgt tcaagcgtga tcatcaggac atgtggtcgt cgtgtgcgtg gacgcatccg
     4681 gacaataccg cgccgttcat cgtcggcggc gcgcagtccc tggatgcgct ttccggttgc
     4741 gtgacccaga tccgcatatc cgacactgcg ttgtcccttt cggaaatgat gtttgcgacg
     4801 ccgaacggcg cggtgcacgg cacggttctt taccatgcgt cgttcgatga agacgccaag
     4861 tgtcatggca ccgtactcga caattacgtc aactgcaata aggaccatgt tgtcgcggga
     4921 acgtttctgc cgacgaatgc cgttcaggcg cgttatgtcc atcccggaaa ggaaatgccg
     4981 gaaggcgctc tcgtgaagaa cttcggtgcc gccgcggaaa acggagagaa tctcttctac
     5041 acctatttcg gaacacagca tgacaacatc ctgcgcgatc tcaatgattt cacgctcgag
     5101 tgctttgtga agatcgatga ggttttcgga acgcttgctg caacaaaccg gttgccgctg
     5161 gtcggtctcg gccactattc cgatgcggac tacaccttcc gctggggatt caagatcgct
     5221 gaagacggac gaccgattgc cgttgtgacc gcgccaaatt attgggaaga cagttactgg
     5281 ggggcaatga gcatcctcga cggtcaatgg catcatctgg cgatgactgt ttcggaaaac
     5341 cccgatgatg gtaaggtgac cgtcaatttc tatgtcgatc atgttcttgc ggcaagcaag
     5401 acggcgacga aatggtattc aaaaggactc acgaccgctc agcgttttca tgtctgccga
     5461 cgtaacgatt tctccggagc gatcgacgag gtacgggtta cgaaggggat cctcgatccg
     5521 cagaccgaga tgctctggca gcggaatcac ggcggaacgt tgctgagtgt ccagtgatga
     5581 tcttgaatca aggcggtttc aaaatcatcg acaccggtga cccgttcgcg cccgggaaat
     5641 acgtttacca actgaaaaag gaacaaacac aatgagggcg aaagccatca acatggtgat
     5701 tttcgggttg tcgatgatga tggccgctgg ccagcccacg ctgtcgtcgg aagtgaaggg
     5761 gcgcgcggca gtcgacacga ggccctacga atttatcgac gcgaagcgcg atgcggacga
     5821 gatcacgccg tggattgatt tcgaagccga taccggctgg cgcgcggagg gtggaaccgc
     5881 acaagcggag ctttcgcagg agcagatgct cttcgggaag ttcacgctca aggtctcgta
     5941 cggaccctgc gccgaggtgc gggtgcgtcc cgccgaaccg ctcgcccttc cggtcgaaca
     6001 cgactggttc ggcgtctgga tccgcaacgc gccggagaac aggggcaagg agaaggggca
     6061 cgaactccac ctcgtgttcc gcaagacgaa cggcgaggag cttcggttgc cgctttcgag
     6121 caggggcaat tcgaagctgg actggccgga ctggtggtac gtcgtaagga agtattcgaa
     6181 ggcggaactg aagacgctcg ccgaaccggg cctcaagttc gacggcttcg tgatcaagcc
     6241 cgcgtccaac gccgtcccct gcgtgatcta tttcgataac atcgcgttct tcaaccgcga
     6301 cgaaacgaag gcattggaaa tcccgcgcgt acccgatgtc catatcccga cgcgtccgga
     6361 aggggcgctc ccggcgtcgc ttgccgaggg cggcgtcaat tacgtccgcc gtgaaggcaa
     6421 gacgacgcgc cttgggtatg agggcccgga cgggaaactc gagtatgtct ggacggggga
     6481 tcttgatacg ctcagggttt catggaacga tgggccggcg tttcgtccta cggcaacggg
     6541 tggcgcgaag acctgtcccg agaaggccgt ctacgcgcct tcgatcgtcg gcaagacact
     6601 cgtccttgaa cttgtcgcac ccgcaggcga gaaggagatc tcgctcggtc acgtcgaggg
     6661 cgcgaaggtc aactaccgga cgctcatccc gatgctcggc gatggttact ggtgggcgaa
     6721 cacgcgttcg cgcgtgagtg tcgtgaacga ctcgaagagc gatctcttcg tgcttgtctt
     6781 tccggattgg tacgtgagcg ccgcttcgag gatcgccgcc gaaagacgtg gggacggaac
     6841 gtatgaccgc gtgatcaagt atatggcgct gacggacggc tcgtacaatg tcgttaagga
     6901 gcgtatctac gtcacggtct cgccggaact gatggagacg ctgcccgtga tcgccaatcc
     6961 cgtttcgccg tggaaacacg tgacgggaaa gaaggcgtgg agccgctatt ccgccagtac
     7021 gaaccgcgcc gaggatatcg cgttcctgac gcggctccac cggtacggga tccgcgatct
     7081 cgtcatcaac gagcacgagg ggtgcatgcg cgacgacggc gaaagcttca ccttccgcga
     7141 caaatccgcg ccaaaaaagg gcggggacaa ggcatggcgg aagtacgccg actatctcat
     7201 caacacgctc ggctattatt acgggcccta caataacttc acggactttc cgccggtcaa
     7261 tgccaactgg cgcttcgacc gggtcgcacg acgaacggtc gagacggacg ggcccgatgc
     7321 cggcggcttc gtgaccgcct ggacgcgatg ttacaatccg aagacggcgt tcggttacgc
     7381 ttcgtgcgtg tattacgccg cgcggcttaa ggagaagtac ggcttcaaca cggcgtactg
     7441 cgatgtccac acctcgaaac cgccgtggag ctacgtcgac tacgacgcgt gcgttcccgg
     7501 cgcgggaacg ttccagaaca cctatcgcgc gtacgcggcg attctcctcg agcagaagaa
     7561 ggcgtggggc ggtcccgtct actccgaggg cgactgccag ttcttctacg cggggttatc
     7621 ggacggcaat tacgcgcagc cgggaatcca tcccgacaaa aatccctgga tcgtcgattt
     7681 caatttgaag cgcatccatc cgctcgaatg cgacttcggc atgggcaacc ccaatatgtt
     7741 caacgtggac aaactgccga ccgattccac gtctccgcgg ctcgtcggcg aaatcgaccg
     7801 gttcctcgcg gcaacactcg cctatggcaa ttcaccgttc ctcgttgtcg gcatgatgtt
     7861 caacccgcag agcgacacgg gcaatcaata tcccgatccg aagcgccgcg cgacaccgga
     7921 actcgggctt ccgtatgtcc tccggagcta cttcatggtg ctcccggcgg cgaaacgcta
     7981 cacgcaggca gaggcggagg agatccgtta tctttccggc aacggcgcgt ggcgtacagc
     8041 atccgcggca cttttcgcag gcgattacga gatgcgccgg atcgcggtgc ggtattcgga
     8101 cgacaccgtc gttgtcgcga acggcgacaa ggtgaaacgg ctcaaggcgg aatatggcgg
     8161 acggaaactt gatctgccgc cttgcggata cgcggtgtgg aacacgacgc atgcactcga
     8221 gatcgaggcg tcggaccgct tcggcgcaag gaccgactat tcggaatccg aggaagcgat
     8281 cttccttgat acgcgcgatg cggtgcagac ggtcgcgttc gaaaaggcgc gcggaaaggg
     8341 acttgccgtc tgccgccgcg agggtgaggg ctgggaggtg attcccgtcc gcggcgaaat
     8401 cgcgttccgg gttccgggcg acaaggtcgt agcgtatgat gaggcgggcc gggaaatcgg
     8461 tacagcggaa atcatccgtg aacaggacgg ttttgtcacc gttcgtccgg tcgccaacgc
     8521 attcagctac aggataatga aaggaaaaat ccatgatgta cccgaagtat agaagggtgt
     8581 ccgctcttgc cgtcgtaatt gccatgatct cgctgctgac cagcagtgcg ccggctgccg
     8641 tttccaatcc gaccgtgatt tggagcaacc ccgatgtttc cctgcagtgg aagacggtca
     8701 tgtccgagaa cctccagttt gcgctcgact ggccagccgg tgccgtcatg gctacgctca
     8761 cgataaaccg gggccgcgac gaatcgatta tcacggtgac cgacacgtcg gcgacgaccc
     8821 ttcccgttcg gatgaatctg cctgcggatg aacagagcga aagcacgctc ctgctgacac
     8881 tggcctacaa ggacgccgcc gctgccgtgc tcgacacgca gacagcgcgt ctcggtctgg
     8941 tgcgcggcat cgatacgaac acggtccgat gcgtgctcga cacgacttca aaactgtgga
     9001 acgtcgtcaa ggtgtccgcc gtcttgccgg tgccggagca gaccgcatcc ctgacgatcg
     9061 acggagcggc ggtcgagccg ctggattccc ccggctggta ctggtggaaa atccgcgagc
     9121 agaaaaatta cgaactcgtt ctcaccgaca cccgctccga agcgaccgct gtcacgctca
     9181 gcggacgccc ccgggtgggc cttgtgatcg tctacaagta aagaaaggcg tttttatcca
     9241 tgaatacgca actcattctc ggtgtgttcg cactggcggc cggtgcggcc ttcgcttccg
     9301 ccacggtcaa ggatgtcaag gtcactcaga actggccgtg gaacggcaag gtcaatatcg
     9361 actacacgat cgaggccccc gcaggcgccc tcgtcgatat cgacatcgtc ctcaggaacg
     9421 gtacacggcg gatcgatatc ggctggggcg cgcatgacgc ctcgcgactc tactcccaga
     9481 cggcaggcac ctatcgcgtc acgtgggatc ctgcgaaaac cgtgggcaac acggctcccg
     9541 tctggttcga gttcacggcc gaggttaccg tcgccaacaa caacgacatg ttcttggtca
     9601 tcgacctcga gggtgacgac ggcgcggggc attatcccgt gacgtttacc gacacccccc
     9661 cagcaggcgg ctggaatact gacgagtaca agaccacgaa gctcgtcatg cgcagaatcc
     9721 cagccggcac ctttacgatg ggttcgccgt acgatgaaat cgggagggcg aacaccagca
     9781 gcagaggccc ggaatatgcc aaaggaccgc gaaccgaggc gctgcatccc gtgacgttta
     9841 ccaacttctt ctatgtcggc atcttcgaga tgaccgaaaa acagtactgc ctcatcgaag
     9901 gcatcggggg cggctcgacg gtggcggtgg cgaactacgc gaacggggtc atcacaaacg
     9961 gcatcgatat cctgagtctg cgcggcgcca actccaagac ccaagacgtc tttccggctt
    10021 acgaggcgga cagtttcttc ggacggctaa acacgaaatt cgccttgacc ggaaagctcg
    10081 cgaattacgt cttcgacctg ccgtcgatct cgcaggcgga atacgcctgc cgcgcgggca
    10141 caaccaccac ctactataac ggctttaaca gctcgagtct gacgaatgac gtcaacctca
    10201 cgccgctggc ctggtaccgc aacgcaacct ggtggcgtac gcgcacggta ggcgggaagt
    10261 tgcccaatgc cttcggtctt tacgacacgc ttggcaacgc aaacgagctt tgccttgatg
    10321 tggtgccgga cgggaccatt ctctggtggc aagaaggggt gccgctcgtc gagccgatcc
    10381 tctacgatcc cgaccactgg ccgtggccgt ctgtagtcca aggcggcgat gaggaacaga
    10441 tcgccaaaga gtgtcgtgcc gcgtgcaaga actcgggggg aagtggcgga cgtaatgttg
    10501 gcttccgcct ggcactggtg gcccgccgca aataatctgg ggaagaaagg aaaatcatca
    10561 tgaagttttt cgtttcgatt ctgtccattg ctctcgcggc agcggtgtcc ggtgccgctg
    10621 aagtcaccca acagatcgtc cgccagaact ggccgtggga taaccgcgta cgcatcgact
    10681 atgttgtcaa ggatgcgacg ggcggcgccc atgacgtgaa tgtcacggtg aaacacggca
    10741 acacctctgt cacgacgggc tggtattcag gcgagttata cggggtttcc gagggggcgc
    10801 atacgatctg gtggaatccg atgaccaacc tgacgaccta tgccgatgcg ttctggaacg
    10861 acctgacggt cgagctgacg ctcgttgatg cgagcgatat gtacatggtc atcgactgca
    10921 cgaccaatgc gtcgctggct gtcagcttta ccaacgcgcc ccccgcaggc ggttggaacc
    10981 agaatcttta caagacctcg aaacttgtcc tgcgtaagat tccggcggga acctatatca
    11041 tggggtcgcc cgagaatgaa cttggtcgca atacgaatcg ctacagagaa gttcaacatc
    11101 aggtgaccct gacgaagtcg ttttatatcg gcattttccc gtttacgcag aaacagtaca
    11161 ccaatcttga ggtgccgacg gattacaatc ccagtccggc ggaccctcaa aaacctgcga
    11221 tcaaccgggg gctcgaaatc tggcgcggca caaactgctg ggataatggc gtctggccgc
    11281 attgcgggga agatggtttc ctgacgcggt tcacttcgcg tgtcacgctg ccagccaagc
    11341 tgaccggcta cgtcttcgac ctgccgacca ttgcgcagtg gcagtatgcc tgccgcgcag
    11401 gtacgatcgg cgcctggaac aatggcacca cgatcacaaa cgtgaccgag gacgccaatc
    11461 tcgatcttct ggcccgttac aaaaagaatc cgaaggtggg atatcatatg tcccatccgg
    11521 tcggctgctt tctgccgaat gctttcgggc tttacgacat gcacggcaac gtgcatgaaa
    11581 tggcgcgtga caaggcatac gggagtcagt tcgccaaatg gtacacgaac gcgccggccg
    11641 tcgatccact ggaatccggt gacccctggc cgtatgtgcc cgcctgcggc ggctattgga
    11701 atgatccggc gggcgaatgc cgttcagcga atatcggctc ggccgggggc aagaatttaa
    11761 ccggcagtga cgctgccgct atccgtatcg cgcttgtcta cgaggattaa
//
LOCUS       DAOSRB010000083        11621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5504_length_11621_cov_8.569082, whole genome shotgun sequence.
ACCESSION   DAOSRB010000083 DAOSRB010000000
VERSION     DAOSRB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5504_length_11621_cov_8.569082"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLZ74_05985"
     CDS             complement(<1..395)
                     /locus_tag="PLZ74_05985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOR97907.1"
                     /translation="MHRCLVTTENLLADTLTLGREASHHLHTVLRVRPDDPVVLFDGQ
                     GRTRRALVRSAERHSVALTAAEPAQTHPAPVCAITLFACISKGHRMDWTVEKAVELGI
                     ARLVPVISEHTVVRLAPGDREGKAERWRRV"
     gene            complement(469..1662)
                     /gene="dnaJ"
                     /locus_tag="PLZ74_05990"
     CDS             complement(469..1662)
                     /gene="dnaJ"
                     /locus_tag="PLZ74_05990"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOR97908.1"
                     /translation="MATQRDYYDILGVGKKASAEEIKKAYRKLAMKFHPDRNPDNKEA
                     TEKFKEVCEAYEVLSDTDKRQRYDQFGHEGMKSAFGPGGFDFRRDFSHMQDIDLQDIL
                     GSFFGGGFGDLFGGNGGGRRRSRNPNAPQRGDDLRFDLEIDLEEAIFGSEREVDLSVN
                     DACPTCKGRGTTSGSGRETCRQCDGHGVVIVGGGFIQMRQTCPVCRGEGTIIRNPCKP
                     CGGSGRVKVPRHLALRIPKGVETGSRLRLSGKGESGLRGGPPGDLYVVLRVRGHKLFQ
                     RQDDDLACTVFVSPVTAILGGDIEVPTPDGHARIKLPAGTPNGKVFRLRGKGMPNLNG
                     GTGDLHVRVEIEVPVRLSSRQKKTLEEFASQTTVENFPETIKQNKAIDTFFARRDALR
                     KHTST"
     gene            complement(1721..2347)
                     /locus_tag="PLZ74_05995"
     CDS             complement(1721..2347)
                     /locus_tag="PLZ74_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOR97909.1"
                     /translation="MIKRQKTPTRHGADGKKSAAPKTEAPQQPDAQPAAAAQPPAAPE
                     PDQSPPEAQELATLKDRYTRLLADFDNFRKRQARDNEEFVRRANENLLSDLLPVVDHL
                     ELALAQTPDPDDPFVIGVRLVYEQIQSLFGRYGLTPIDAKGEPFDPAFHEAISQASSL
                     TVPTQTVIEQFRRGWMLSGRLLRPAQVVVSSGKPEPEQVEAESVSVSD"
     gene            2745..3752
                     /gene="rpsB"
                     /locus_tag="PLZ74_06000"
     CDS             2745..3752
                     /gene="rpsB"
                     /locus_tag="PLZ74_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOR97910.1"
                     /translation="MDMPVSSLTGLTLKDLLDAGLHFGHQTKRWNPKMKRYIFDKRNG
                     IHIIDLTQTVTLIDEAAAFLREAVLSGKKILFVATKKQAQETVREAAEACGQFHMTER
                     WLGGTLTNNQTIRRSVRRMRQIEAMGRNNNGVLSVHKKEASSLRRELEKLQKNLGGVA
                     DMESKPAALFIVDICREQNAVKEARRLGIPLVAIVDTNADPDPVQYVIPGNDDAVRGI
                     KLIADAFAKVIKNANDEYSRIAAERQQQKEAELASQEAQERAARKAARKKEAAGAEGK
                     ADKAKTKLAETRKRAAKVAKETVKAKAAAVEAAAPAPEAAAPAVVEAAPAAEASAPAA
                     E"
     gene            3829..4668
                     /gene="tsf"
                     /locus_tag="PLZ74_06005"
     CDS             3829..4668
                     /gene="tsf"
                     /locus_tag="PLZ74_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOR97911.1"
                     /translation="MAEITVAAINDLRQRTNAGMMDCKKALTDANGDIDAAIKALREK
                     GLAIQVKRADKESKEGIIMARTSEDGKTIGMAEVNTETDFVAKTDNFKQFVKAVTDKT
                     LEGVEDVSAALKDELMTIVSQTGENVKIRRTALYKLSGTGKVASYIHMGGKVGVLVEV
                     GCGNEATATSAAFAELVHDLTLQIAAAAPRWITPDEVPAEVVEAEKDIYRNQVQGKPA
                     NIVENILKGKIQKFYSDVCLIHQPFIKEPKQTVTDVVNACAKATGDTITIKRFVRYQL
                     GAA"
     gene            4686..5492
                     /gene="proB"
                     /locus_tag="PLZ74_06010"
     CDS             4686..5492
                     /gene="proB"
                     /locus_tag="PLZ74_06010"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOR97912.1"
                     /translation="MNVEHRYREFLPEARRVVVKIGTRVIAHPSGRPDMRPLKGLVRQ
                     IARLHRKGYELLVVSSGAIGAGMEALGMTVRPTSVPDLQMCAAVGQARLMAHYEELFD
                     TEKITIGQVLLTHADFQHRIRFTNAKRTLEHMIRRDVIPIINENDAVADEEIKAGLSL
                     GDNDYLAALVVKLVRADLLVILSTVDGVLDAKNRRVACIENLNEAFALVMPTEEGGLS
                     KGGMDSKLRAAQIAVRAGCSVVVACGRTPDVLTQVMEGCDTGTLILSSTL"
     gene            5506..6783
                     /locus_tag="PLZ74_06015"
     CDS             5506..6783
                     /locus_tag="PLZ74_06015"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOR97913.1"
                     /translation="MTPNLNEPNLAETLRLIGDQAQAAARRMVGLSTRRKNAILNAMT
                     EALEARRDQIREANDRDLEAARSAGIPAALLDRLTLTGARIDAFIQGVREVALLPDPV
                     GRLIWRRNRPNGLRIEKRRVPLGVVAIIYEARPNVTADAAVLCIKTSNAVILRGGKEA
                     FHSNLAIARALQEGGERAGLPPHAVQLIETTDRGAVRELVRLEDQVDVVIPRGGESLI
                     RAVMEHARVPVLKHYKGVCHVFVDASADLAQALAIAENAKCQRPGVCNAAETLLVHRD
                     VADAFLPQLGELAARRGVTLRGCPATRAVLPACEPATEADWTAEYLDLILAVRVVRDL
                     KTAIAHINTFGSHHSDAIVTADTRSANRFLKEVDSAAVYVNASTRFTDGGEFGMGAEM
                     GISTDKLHARGPMGLEELTTYKYRITGTGQVRG"
     gene            6791..7828
                     /locus_tag="PLZ74_06020"
     CDS             6791..7828
                     /locus_tag="PLZ74_06020"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOR97914.1"
                     /translation="MKNKKPARRNARITQVAETLRAAKRVLITGHVRPDGDSLGGMIA
                     LAHLLNRAGVRAVATAARTGLGGPGFLTGVKKLLAPEEAVKRSFDLMVALDCGAFDRM
                     PEALHPLASRLPLINIDHHLTNTLFGTLNWIDGRASSTGEMLWRLARKAGWPLDTVSA
                     EALWVALITDTGRFAHEQTRPATLRCGADLLRYGVRTAFINDKLYCSFSRVAMELKRR
                     AFRTLTVWGEDNTVALVTLTGQDFEETGGTKADAEDVIEIPRSLVNNRVALFFYGSEE
                     DNAETRVSIRTRRPLDATVLAKQFGGGGHSCAAGCTIRQPLSQAKRLFRKAVADLLEK
                     NPSDDASLSSS"
     gene            complement(7883..9505)
                     /locus_tag="PLZ74_06025"
     CDS             complement(7883..9505)
                     /locus_tag="PLZ74_06025"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOR97915.1"
                     /translation="MTFDLILRGGQVADGSGAPARPADVAVKGDRIAAVGDLASARAT
                     REIQAAGLMVAPGFIDAHAHSDAYLLIEPDAPSKLTQGITTEVNGQCGGSVAPRLGEA
                     RLSSDWASQHYPRFSPAGGVEANPDPGPTWGTVAEYRRLFDAVQPAINTIQFIGHNTL
                     RAGVMGYAPRPAAAADLREMIRRLEQALEEGGWGLSTGLLYPPGKYAAGEEVAALVRA
                     AALRGGMYATHMRSEGDELLESVGEVLRLARDTGIRVQISHLKTSGRDNWPKIEALLE
                     MLRRARDAGVALHGDRYPYTASGTDLDVVLPEWAAAGGRDAILANLRSPAARRRMEEE
                     LDANPRDWSAVMIGGGWSAEVRRFSGRTVADAAAELGLSPGSVVCRFIERDETRTGAF
                     FFVMCEANLRHLYREPWIMPGSDASLRAPWGPLGLDHPHPRAYGTMPRFVRIVTGREE
                     GVPAVCGIEEAVRRMTSLPACAFGIRDRGTLIPGAFADVTVFDPETFRDTATFAVPHQ
                     FAAGVRQVIVNGAVAFDNGRFTGERRGRFLSR"
     gene            complement(9516..10079)
                     /locus_tag="PLZ74_06030"
     CDS             complement(9516..10079)
                     /locus_tag="PLZ74_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97916.1"
                     /translation="MAQEWNIRPRGHVCSICARPFEDRKPCVSVLRDVDGTYERFDCC
                     PACWRDTPREWEPFSFWEGEYEAPAPITPKQEVVKRETAEGLLRRLITLEDPAMRDAV
                     YVLAVMLERGRQLVERDAKPHESGGILRVYEHKKSGDTFVVLDPRLRLDQLGAVQQQV
                     VALLSGTHALDVQPETAAPAEPVSETE"
     gene            10265..11368
                     /locus_tag="PLZ74_06035"
     CDS             10265..11368
                     /locus_tag="PLZ74_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOR97917.1"
                     /translation="MKIKKRNQGGNGEGLGRRSFIGTIAGAAAALSAGCKATAAAKGQ
                     PAAASRAARRGAQRLSLDRLRQFEALGYGMFISYDIQAFYKGTIHGKVTDEMRRIPAS
                     CYAPDKLDVGQWIAVARDAGMKYAVLTAKRHPGFCLWPSRHTDYTVANSGNRTDVVEQ
                     YVKACAQCGIVPGIYYPAVDFHHLHGRPPQDDWRYVTSLHQTFITDQITELLTEYGEI
                     GEVWIDIPKVLGRGYRTFLYEHIARLQPNAVILMNSGIQDGLKINVEKVWPTDLISIE
                     VKGPAKGGYQKWRTVEGREYYLPGEYCNSIMPKWWWREGDLPRPDQDVLKEFQAARAV
                     GVNVLLDAPPDNHGLIPKETVDALMRLRRNAGL"
BASE COUNT         2224 a         3664 c         3711 g         2022 t
ORIGIN      
        1 acccgtcgcc accgttccgc cttcccttcc cggtcgccgg gagcaagacg tacgaccgta
       61 tgctcggaaa tcaccggcac cagccgtgcg atgccgagtt cgacagcctt ctcaaccgtc
      121 caatccatcc ggtggccctt gctgatgcag gcgaacaggg tgattgcgca gacaggtgcc
      181 ggatgggttt gggcgggctc ggcagcggtc agggcgacgc tgtgccgttc agccgatcga
      241 acgagggcac ggcgtgtgcg cccctgcccg tcgaagagga cgaccggatc gtccggccgc
      301 acccgcaaca cggtgtgaag atggtgcgac gcttccctgc cgagcgtcag cgtgtcggca
      361 agcagattct cagtcgtgac aaggcaacgg tgcatgagcg acgggtccgg tgatccgtta
      421 caggttgtgc ggtcggtaga ccggcctccg acacgcaggg ttccgcggtc atgtggatgt
      481 gtgtttacgc agggcgtcgc gacgcgcgaa aaaggtgtcg atggctttgt tctgttttat
      541 cgtttccggg aaattctcta ccgtggtctg gctggcgaac tcttcgagcg tctttttctg
      601 ccgggaagag aggcgcacgg ggacttcgat ctcaacgcgg acgtgcaggt cacccgttcc
      661 gccgttgaga ttaggcatgc ctttgccgcg gaggcgaaaa accttgccgt tgggggtgcc
      721 cgccggcagc ttgatgcgcg cgtggccatc aggcgtcggc acctcgatgt ccccgccgag
      781 tatggcggtc accggcgaaa cgaaaaccgt acaggcgagg tcatcatcct gccgttgaaa
      841 cagtttgtgt ccgcgcacac gcaacaccac atacaggtca ccgggcgggc cgccccgcag
      901 accgctttcg cctttgccgg aaagacgcag ccgcgatccg gtctccacgc ctttagggat
      961 ccgcagcgcg agatggcgcg gcaccttcac gcgaccggaa ccgccgcaag gtttgcaggg
     1021 gttgcggatg atcgtgccct cgccgcggca gaccgggcag gtctgccgca tctggatgaa
     1081 cccgccgccc acaatcacca caccgtgacc atcgcattgg cggcaggtct cgcgtcccga
     1141 ccccgaggtg gtgccgcgtc ccttgcaagt tgggcaggca tcattgaccg acaaatccac
     1201 ttcgcgttcg gatccgaaga tcgcctcctc caaatcgatc tccagatcga aacgcaggtc
     1261 atcgccgcgc tggggcgcat tgggattccg agaccgccgt cgtccgccgc cgttcccccc
     1321 gaaaaggtcg ccgaacccgc cgccgaagaa actgccgaga atgtcctgca ggtcgatgtc
     1381 ctgcatgtgg gagaaatcgc gtctgaaatc gaacccgccg ggtccgaagg cggacttcat
     1441 gccctcgtgt ccgaactggt cataccgctg acgcttgtcc gtgtcgctga gcacctcgta
     1501 ggcctcgcag acttccttga acttttccgt cgcctcttta ttgtcgggat tgcggtccgg
     1561 atggaatttc atggccagtt tgcggtaggc ctttttgatc tcctcggcag aggctttctt
     1621 gccgactccg agtatatcgt aataatcgcg ttgggtagcc atagcggaaa agggattagg
     1681 gtaacatgtt tccggtcgag tacggttggc tgcggaacat tcagtctgag acgctcacgg
     1741 actcggcttc gacttgctcc ggctccggtt tgccggagga aacgaccacc tgcgcggggc
     1801 gcaggaggcg tccggagagc atccagccgc gccggaactg ctcgatcacc gtctgcgtcg
     1861 gcacggtcaa cgaactcgcc tgcgagatcg cctcatggaa cgccgggtcg aagggctcgc
     1921 ccttcgcgtc gatcggggtg agcccgtacc gaccgaatag cgactggatc tgctcgtaaa
     1981 ccagccggac ccctatcaca aaggggtcgt cggggtccgg ggtttgggcc agcgcgagtt
     2041 caagatgatc gaccaccggc aacaggtcgc tgagcagatt ctcgttcgcg cgccggacaa
     2101 actcctcgtt atcgcgcgcc tggcgtttgc ggaagttatc gaaatcggcg agcaaccgcg
     2161 tatagcggtc tttgagcgtc gcgagttcct gtgcctccgg tggggactga tctggttctg
     2221 gtgcggcggg aggctgtgca gcggccgcag gctgcgcgtc cggctgttgc ggggcctcgg
     2281 ttttcggagc cgccgacttt ttgccatccg cgccatgtcg agttggggtc ttttgtcgtt
     2341 tgatcatcag gcaatctccc ctcgggtttt cgaggcaccg gtaccgggtt ggaagggaac
     2401 cgggaacccc gcatccggtt caaaaaatca tacgggaaaa gggaaatagt caaccatact
     2461 cacccatgat agtcaagcag gccgaatcgg tttttcggga ggcggtgaat caaaaaacag
     2521 ccggggcctt gcgctctggc cggaaaaagg gtatctttct tttttattcc ctagcgcggg
     2581 atgccgggtg gctcccctgc gtggaagcga aggcgcggcc ggttacagga ccggtccgtc
     2641 aaagcggatt aacccgggaa gcttcgggtg cggtatcgtg ccggccggtc catggccgtt
     2701 ggcgcgacgc gttgccgagg atcccagttg agaaagtaaa cccaatggac atgcctgtat
     2761 cgagcctgac cgggctcacc ctgaaagacc tgctggatgc gggtctgcac ttcggccacc
     2821 agaccaaacg gtggaatccc aaaatgaagc gctacatctt cgataagcgc aacgggattc
     2881 acattatcga tctcacccag acggtaacgc tgattgacga ggcggccgct tttttgcgtg
     2941 aagccgtcct gagcggaaaa aagattttgt tcgtggccac caagaagcag gctcaggaga
     3001 cggtgcgcga ggcggcggag gcctgcggtc agttccatat gacggagcgc tggctcggcg
     3061 gcacgctcac caacaaccag accatccgtc gcagtgtccg ccgcatgcgc cagatcgaag
     3121 ccatggggcg gaacaacaac ggcgtgctct cggtccacaa gaaagaggcc tccagcctgc
     3181 gccgcgaact ggaaaagctc caaaagaact tgggcggcgt ggcggatatg gaaagcaagc
     3241 cggccgcgct cttcatcgtc gatatctgcc gcgagcagaa tgcggtcaaa gaggcgcggc
     3301 gtctgggcat tccgttggtg gccattgtgg ataccaacgc cgacccggac ccggtccagt
     3361 atgtcatccc cggtaacgat gacgccgtgc gcggcatcaa actgattgcg gacgcgttcg
     3421 cgaaggtgat caagaacgcc aacgacgagt attctcgcat tgcggccgaa cggcaacagc
     3481 agaaagaggc ggagctggcc agccaggagg cgcaggagcg cgccgcgcgc aaagctgcgc
     3541 gcaagaagga ggcggctggc gctgagggca aggccgacaa ggccaagacc aagctcgccg
     3601 agacccgcaa gcgtgccgcc aaggttgcca aagaaacggt gaaggccaaa gccgccgctg
     3661 tcgaggctgc cgcgcccgcc cctgaggctg ccgcgcccgc tgttgtcgag gccgcgcccg
     3721 ccgctgaggc ttccgcgccg gccgccgaat aacccgctgg tccgtgccgt gccggcagga
     3781 taccgacacg gccgacccgc attcactttt cacttttagg aggatatcat ggcagagatc
     3841 acagttgcgg caatcaatga ccttcggcag cgtaccaacg cgggcatgat ggattgcaag
     3901 aaagccctta ccgatgcgaa cggcgatatc gacgcggcca tcaaggcgct gcgcgagaag
     3961 gggcttgcga ttcaagtcaa acgtgccgac aaagagtcga aggaaggcat catcatggcg
     4021 cggacctcgg aggacggcaa gaccatcggc atggctgaag tcaacaccga aaccgacttc
     4081 gtggccaaga ccgacaattt caagcagttc gtcaaggccg tgaccgataa gaccctcgaa
     4141 ggggttgagg acgttagcgc ggccctgaaa gatgagctga tgaccattgt ctcgcagacc
     4201 ggcgagaacg tgaagatccg gcggaccgcg ctctacaagt tgagtggcac aggcaaggtt
     4261 gcctcctaca tccacatggg tggcaaggtc ggcgtgctgg ttgaggtcgg ttgcggtaac
     4321 gaggccacgg caacgagcgc ggcgttcgcg gagttggtgc acgacctgac gctgcaaatc
     4381 gcggcggccg caccgcgctg gatcacgccg gacgaggttc cggctgaggt ggtcgaggct
     4441 gaaaaggata tctaccgcaa tcaggtgcag ggtaaacccg ccaacatcgt ggagaacatc
     4501 ctcaagggca agatccaaaa gttctattcg gatgtctgcc tgatccacca gccgttcatc
     4561 aaagagccga agcagaccgt caccgatgtg gtgaacgcgt gcgccaaggc caccggcgac
     4621 acgatcacga tcaaacggtt cgtccgctac cagctcggtg cggcgtaacc cggttgcgtt
     4681 cgtttatgaa tgtggaacac cgctaccgtg agttcctgcc ggaagcccgg cgtgtcgtgg
     4741 tcaaaatcgg cacccgggtg atcgctcatc cctccggacg gccggacatg cggccgctca
     4801 aaggactggt gcgccagatc gccagactgc accgcaaggg atacgagctg ctggtggtct
     4861 cctcgggtgc gatcggcgcg gggatggagg cgctgggcat gaccgtccgg cccaccagcg
     4921 tgccggatct ccagatgtgc gccgcagtcg ggcaggctcg gcttatggcg cattacgagg
     4981 aattgttcga caccgagaag atcaccatcg gccaggttct cctcacgcat gcggatttcc
     5041 agcaccgcat ccgcttcacc aacgccaagc ggacgttgga gcacatgatc cgccgtgatg
     5101 tgatcccgat catcaacgag aatgacgcgg tggcggatga ggaaatcaaa gccgggttgt
     5161 cgctgggcga caacgactat ctggccgcgc tggtggtcaa gctggtgcgg gccgacctgc
     5221 tggtgatcct cagcacggta gacggtgtgt tggacgcgaa gaatcgtcgg gtcgcctgca
     5281 tcgagaatct caacgaggcc ttcgcgctgg tcatgccgac cgaggagggc ggtctctcca
     5341 aaggcggtat ggactccaag ctgcgggccg cgcagatcgc ggttcgcgcc ggatgcagcg
     5401 tggtggtcgc ctgcggccgt accccggacg tgttgactca ggtgatggaa ggctgcgaca
     5461 ccggcacgct gatcctcagc agcaccctgt aacccggagg cggatatgac accaaacctc
     5521 aacgaaccga atcttgcgga aaccctacgc ctgatcggcg atcaggcaca ggccgccgca
     5581 cgccgcatgg tggggctctc gacccgccgc aagaatgcga tcctgaatgc catgaccgag
     5641 gcgctggagg cgcgtcgcga tcagatccgt gaggcgaatg accgggatct ggaggccgcg
     5701 cgttccgccg gaatcccggc ggctcttctc gaccgcctga cgctcacggg ggcccggatt
     5761 gacgcgttca ttcagggtgt gcgcgaggtg gcgctgctgc ccgatccggt cggccgcctg
     5821 atttggcggc gcaaccgccc caacggcctg cggatcgaga aacggcgcgt gccgctgggt
     5881 gtggtcgcca tcatctacga agcgcgaccg aacgttaccg ccgacgccgc cgtgctctgc
     5941 atcaaaacct cgaatgccgt gatcctgcgc ggcggtaagg aggccttcca ttctaacctc
     6001 gccatcgcgc gtgcgctgca ggaaggcgga gagcgcgcgg ggttgccgcc ccacgccgtg
     6061 caactgatcg aaaccaccga ccgcggggct gtgcgcgaac tggtgcggct ggaagaccaa
     6121 gtcgatgtcg tgatcccgcg cggcggcgaa tcgctgatcc gcgccgtgat ggagcatgcc
     6181 cgcgtgccgg tgctcaaaca ctacaagggg gtctgccatg tgttcgtgga cgcttcggcc
     6241 gaccttgccc aggcgctcgc cattgccgaa aacgccaagt gccagcgtcc cggggtctgc
     6301 aacgccgccg aaaccttgct cgtacaccgt gacgtcgcgg acgcgtttct gccgcaactc
     6361 ggagagctgg ccgcgcggcg cggcgtgacg ctgcggggct gtcccgccac gcgggccgtg
     6421 ctgcccgcat gcgagccggc cacagaagcg gattggacgg ctgagtacct tgatctgatc
     6481 ctcgcggtgc gcgtggtccg tgatctcaaa accgccatcg cccacatcaa caccttcggc
     6541 tcgcaccatt cggatgcgat cgtcaccgct gacacacgca gcgccaaccg cttccttaaa
     6601 gaggtggact ccgccgccgt atatgtgaat gcctccaccc ggtttaccga cggcggtgag
     6661 ttcggcatgg gagcggagat ggggatcagc actgacaaac tgcacgcccg cggcccaatg
     6721 ggcctggaag agctgaccac ctacaagtac cggatcaccg gaacggggca ggtccgcgga
     6781 taggatgcgt atgaaaaaca agaaacccgc ccgccgtaac gcccggatca ctcaagtggc
     6841 ggaaacgctt cgcgcggcca aacgcgtgct gatcaccggc cacgtgcggc cggacggcga
     6901 ttcgctcggc ggaatgatcg ccctcgccca tctgcttaac cgggcggggg tccgggccgt
     6961 ggccaccgcc gcgcgtaccg gactcggcgg ccccggcttt ctgaccggcg tcaaaaagct
     7021 gcttgcgccc gaagaggcgg tcaaacgttc gtttgacctg atggttgcgc ttgactgcgg
     7081 cgcgttcgac cgaatgccgg aggcgctcca cccgctggca tcccggcttc ccttgatcaa
     7141 catcgaccac cacctcacca acaccctttt cggcacgttg aactggatcg acggacgcgc
     7201 ctcctcgact ggcgagatgc tctggcgttt ggcccgcaag gccggctggc cgcttgatac
     7261 cgtctcggcc gaagcgctgt gggtcgcgct gatcaccgac accggacggt ttgcgcatga
     7321 gcagacccgg cccgcaaccc tgcggtgcgg tgccgacttg ctgcggtacg gggtccgtac
     7381 cgcgttcatc aacgacaaac tctactgctc gttcagtcgg gtcgcgatgg aactgaagcg
     7441 gcgcgccttc cgtacgctca cggtgtgggg ggaggacaac acggtggcgt tggtgacgct
     7501 gaccgggcag gatttcgagg agaccggcgg aaccaaggcg gatgccgagg acgtcatcga
     7561 aatcccgcgt agcctggtca acaaccgtgt ggcgctcttt ttctacggca gcgaggagga
     7621 caacgcggag acacgcgtca gcatccggac ccgcaggccg ctagacgcca ccgtgctggc
     7681 caaacagttc ggcggcggcg gccactcgtg cgcggccgga tgcacgatcc ggcaaccgct
     7741 ctcccaagcc aagaggctgt tccggaaagc cgtggccgac ctgctggaaa aaaacccgtc
     7801 cgacgacgcg tccctctcgt cctcgtgaac gtcgacctgc gacccgcggc agggacacct
     7861 ccgaaccgtc tgtccgcctg aatcagcggg agaggaagcg accgcggcgc tcacccgtga
     7921 accggccgtt gtcgaacgcg acagcaccgt tgacgatcac ctggcgcaca cccgccgcga
     7981 attggtgcgg cacggcgaaa gtggcggtgt cgcgaaacgt ctccggatca aacaccgtca
     8041 catccgcgaa cgcgcccggg atgagcgtgc cgcgatcacg gatgccgaag gcgcaagcgg
     8101 gtagcgaggt catgcggcgc acggcctctt cgattccgca gaccgccgga accccctctt
     8161 cccgacccgt gacaatccgc acgaagcgcg gcatggtgcc gtaggcgcgc gggtgcggat
     8221 ggtcgaggcc cagcggtccc cacggcgcac gcaacgaggc gtcgctaccg ggcatgatcc
     8281 acggctcgcg gtagaggtgc cgcaggttcg cttcgcacat gacgaagaag aacgcgcctg
     8341 tgcgcgtctc gtcacgttcg atgaagcggc agaccacgct gcccggtgaa aggcccagtt
     8401 ccgccgccgc gtccgccacg gtgcgtccgc tgaaccgccg cacctctgca ctccagccgc
     8461 cgccgatcat gacggctgac cagtcgcgcg ggtttgcgtc gagctcctcc tccatgcgtc
     8521 ggcgcgccgc aggggaacgg aggttggcga ggatcgcgtc gcgtcccccg gctgcggccc
     8581 attccggcag caccacatcc aaatccgttc cggatgcggt gtacgggtaa cggtcaccgt
     8641 ggagcgccac tcccgcatcg cgcgcgcgac ggagcatctc gagcagcgct tcgatcttcg
     8701 gccagttatc gcgaccggag gtcttcaggt gcgagatctg cacacggatt ccggtgtcgc
     8761 gagcgagacg cagaacctcg ccgaccgatt ccagcagttc gtctccctcg ctgcgcatgt
     8821 gcgtggcgta catccccccg cggagcgcgg ccgcgcggac cagtgccgcg acctcctccc
     8881 cggcggcgta tttaccgggc gggtagagca gaccggtgct caatccccat ccgccctcct
     8941 ccagcgcctg ttccaatcgc cggatcattt cgcgaaggtc cgctgccgca gccgggcgcg
     9001 gggcataccc catgaccccc gcgcgcaagg tgttgtgacc gatgaactgg atggtgttga
     9061 tcgctggctg caccgcatcg aataaccggc ggtattcggc gaccgtgccc caggtcggtc
     9121 cgggatccgg attcgcctcc acgccacccg caggggagaa gcgtgggtaa tgttgcgacg
     9181 cccaatcgga ggagaggcgc gcctcaccca gccgcggcgc gaccgatccg ccgcactggc
     9241 cgttgacctc cgtggtgatg ccctgcgtga gtttgctcgg agcgtcgggt tcgatcagca
     9301 gataagcgtc cgaatgggcg tgcgcgtcga tgaatcccgg cgcgaccatg aggcccgcgg
     9361 cttggatctc ccgcgtagcc cgcgcagagg caagatcccc gaccgccgcg atacgatccc
     9421 ccttcaccgc gacatctgcg ggccgcgctg gcgcaccgct tccgtccgcc acctgtccgc
     9481 cacgcaggat gagatcaaag gtcatgcggg agacctcatt cggtttcgga caccggttcc
     9541 gccggggccg cggtttcggg ctggacgtca agcgcgtgcg tcccgctgag cagcgccacc
     9601 acctgctgct gcaccgcacc caactgatcg agccgcaacc gggggtcgag caccacaaag
     9661 gtgtcgccgc tcttcttgtg ctcatacacg cgcagaatgc cgccgctctc atgcggtttg
     9721 gcatcccgct caaccagttg gcgaccgcgc tccaacatca cggccagcac atagaccgca
     9781 tcccgcatgg cgggatcctc cagcgtgatc agccgccgca gcaacccctc ggcagtctcc
     9841 cgcttcacca cctcttgctt cggcgtgatt ggcgcggggg cctcgtattc gccctcccaa
     9901 aagctgaacg gctcccactc gcgcggcgtg tcgcgccagc aggcgggaca gcagtcaaac
     9961 cgctcatacg tgccatccac atcgcgcaag accgacacgc acggcttccg gtcctcgaac
    10021 ggccgggcgc agattgaaca gacatgccct cgcgggcgga tattccactc ttgtgccatg
    10081 ggggcagtat aacaaaaaac gcgcggacag acacccgcgc gtttagaaat cgggatgcgg
    10141 ggcgtttttt aacgcaaaga gcgcatcggc caaaaccggc ggcgtgactt tttgatggta
    10201 caaaaaccgg aacccggaat cagtaaggtt gttctttctc gatgttttgg atacaatcaa
    10261 aaccatgaaa atcaaaaaga gaaaccaggg cggcaatggc gaagggctgg gacgccgtag
    10321 ttttatcggt accattgccg gtgccgcagc ggctttgtcg gcagggtgta aggcgaccgc
    10381 cgctgccaaa ggccagcctg cggccgcttc ccgtgccgcc cggcggggtg cgcagcggct
    10441 ctccttggac cggctccgcc agttcgaggc gctgggctac gggatgttca tctcgtacga
    10501 cattcaggct ttttacaagg gaaccatcca cggcaaggtc acggatgaga tgcgccgcat
    10561 tccggcgtca tgttatgcgc ccgacaaact ggatgtcggg cagtggatcg cggtggcgcg
    10621 cgatgctggc atgaaatacg ccgtgctcac ggccaaacgc catcccggat tctgtctctg
    10681 gcccagccga cataccgact acaccgtggc caacagcgga aaccggacgg atgtggtgga
    10741 gcagtatgtg aaggcgtgtg cgcaatgcgg tattgtgccg ggaatttact atccggccgt
    10801 ggatttccac catcttcacg gtcggccgcc gcaggatgac tggcgctatg tgacctcgct
    10861 tcatcagacc ttcatcacgg atcaaatcac ggagttgctg acggagtacg gtgagatcgg
    10921 cgaagtctgg attgacatcc ctaaagtgct cggacgcggt taccgcactt tcctctacga
    10981 gcatatcgcg cgtttgcaac ccaacgcggt gatcttgatg aacagcggga ttcaggatgg
    11041 tttgaaaatc aacgtggaaa aagtctggcc cacggatttg atctcgattg aggttaaagg
    11101 cccggctaag ggaggatacc agaagtggcg gaccgtcgag ggccgggagt attacctgcc
    11161 gggcgaatac tgcaattcca tcatgccgaa atggtggtgg cgcgaaggtg acctgccccg
    11221 gcccgatcag gatgtgctta aggaatttca agccgcccgc gctgttggag tcaacgtcct
    11281 gttggacgcg ccgcccgaca accatggcct gattccgaag gaaacggttg acgcgctgat
    11341 gcggttgcgc cgcaatgcgg ggctgtgagc ggtcagaggg cgtgtgccgc gcccgcggcg
    11401 gtttcggggg aaccgccccg ccacgcgggg tagggacgcc cgcttctccc gtcaggcctt
    11461 atgtcgaccc gcgacccgcg acgagcgacc tgcggtgtcg gaaacgcggg cgtccctgtc
    11521 gatcgtcgcg ggtcgaatgt cgcgggtcga cgatcccgat cccgatcccg atttggatga
    11581 aaccatgaaa cacataaaac ttcatgaaaa tcgggaattc c
//
LOCUS       DAOSRB010000084        11484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5568_length_11484_cov_6.346924, whole genome shotgun sequence.
ACCESSION   DAOSRB010000084 DAOSRB010000000
VERSION     DAOSRB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5568_length_11484_cov_6.346924"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..4378)
                     /locus_tag="PLZ74_06040"
     CDS             complement(170..4378)
                     /locus_tag="PLZ74_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97918.1"
                     /translation="MKQLITVITTAALAAAVSASDGYWFVNQMTYAPDSLKWSNTKYW
                     LDGVPAQNGGVFTHASASDSMSQWVSAGVYQDVAGLEISGMAFPYSGAFLLAGNQPIT
                     FVGDAPYLRPKDEEGKYVDQKIPFLGNNNAFEKTGRGTLRVYDGAFYSGFTNVTFKHG
                     LVAVYAKSGTALTDGRVTFEGNEIRFEPVAAEGSAAALTIATNSAVALKGSVRFSLKK
                     GSNASASLTIGEVVREDVFPGTLYLAPNGGTGTLGTSEKFFLTNPAALVHAGSGMVSP
                     VIALGGPNTVSTPVCFARYDASNGLVPATDLYHDGFAGADSNAIVRITANTTLSADTA
                     IHALVIDGNAVLTLNPGVTLTIGDGTSPAGIILQGFTTTKTCISGGAIDFRGSEGIVW
                     VQDNNSKTLQTIDTEIRGTAGVTFTGRNNVDYVNTILSGTYANSGDIRAYWGRVQLYA
                     DLTSAPAVRVFGDRLCGAQFLNYTKTAISELSLAGFGISTYHGRAVFTGTGEDFSTNI
                     ARVVLADDAGIRMNKNLQFDCPVTGPGGLVILQDSNKLTLNSTNTYLGVTRLETGTLT
                     LGANGTFGAGEVINNTTITVNKNADFVISNAVSGVGSWINSGTNTLLFASPSVTMGGL
                     TTQGDIGIAGAATRLGNLAQSSERAITPIAEGADAPAMAEIGTSSDILYGGYLRDNGT
                     NLIGLTKIGSGTLGIAKPQSYSGPTVVREGTLKLYRELIPCEVDIAYHLDANDAASVQ
                     TDETTGAVTGWVDRVTGVAFKQTWDGLPCPVYSNGVGGAIQSKPAIYFNGWTNCLVAN
                     KAFATKTVLILFQTTGYSVAGGGMDCLWANTRGDHGSRFLRLASATSIDTGTEGSVFH
                     YSTLANLSINGVAGVNTFTPNEPTLLSAFRTDDFGAGNNVIGNNYQGRSLCGFIGEVI
                     AFSRILSRDEIKAVENYMWQKWMGTELHAEAPAPVHGTLPAGTTLSVEPGAAFDLNGI
                     DQTVSTLSGIGGNIINSGAETATLTVTGTNGFRGTIGEKVNLALCGAADMALRVEAGT
                     VSFGGASAVTVFNDLPPQDGLQYWLDATDAATVRRSADGYVTNWVSKAGTEGLAFGLE
                     KQAWDAETSQPPTYTGSINGQSAVFFGNATTNWMRSNKSIAVQTLVMVFQAFQNQMNM
                     AGYWGVNSGGDLGVRGSSTNFSYGTVGNCFVGAEAWHNGIALPASASGFDYALNTPVT
                     AHIESPVKAGPGNNAIGGFYVNSPTDTRRFRGAIGEVIAYNRMLTKAERDQLEHYLAR
                     KWRTPGYTVNTEVFAAGSEVTLTAGASLNLRGAPLVVARLTGGGEVTGDVTLTDTYTV
                     TLDETGGCPNPLRVTGDLTFTGCRLVVVGTLKNALHKVKIAEADGVLTGLPSWDEPQP
                     GWQVVREGGAVYLYKELGTTVILR"
     gene            4837..7044
                     /locus_tag="PLZ74_06045"
     CDS             4837..7044
                     /locus_tag="PLZ74_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR97919.1"
                     /translation="MRFAMMGMVLGLVSGVWAEVTIHVAPNAPAGGDGRRRSPYATLE
                     QARDHIRAARKEGLIARDEPVTVLLEPGVHTLTQSFELTREDGGSEAAPVVYRAAKRG
                     RARLRGGLTLPADAFQPVTNGAVLNRLDPAVREHIRVCDLTSRVPGGFEPFKNAYSGT
                     PAAPWLYHNRKPLTLARWPNADAPQGGWAGFTKAVETGKPIPDSPDPALRKPRPGSFE
                     FADPRPARWNLEEGVWLMGYWTHDWSGEVIRIASYDPDKKVIALAAPHGYGIAAGTWG
                     SAVRRFFALNLMEELDAPGEWYLDRTSKQLYVYPEGETEEGGEWVLATLTQPMLAVSD
                     AAHIRVLDLAFEYSHCAGVVIILKNSEQVEVAGCVVANHAGSGISVSGRANTVRSCDL
                     YQLGKAGLSLNGGDRKKLIAAKNLAVNNHIHHYGLFQRTYAAGIALGGCGQIARNNCI
                     HDAPHNAVLYGGNEHLLELNEVYRVVMETGDAGAFYTGRDWTSQGNVLRHNFVHTLGR
                     GDAKHVNTMGFYLDDCDCGDRIEGNVFWRAGRAIMIGGGRDNPVINNLIVECPIGLHI
                     DSRGMTWKYWNNTNDPNWRLDAKAQEFDYRNPPWSVRYPKLASIMDECPREPLNNPAR
                     RNVFVDCVDTCSFGGKVMDLLHKMELEDNLIVNTVGTNRVGKLREGINKGFRTVSGTP
                     DKPFDPGFEDAPNGNFALRRNARLFTELPAFERIPFERIGLYTDTFRRKLPRR"
     gene            complement(7125..8471)
                     /gene="miaB"
                     /locus_tag="PLZ74_06050"
     CDS             complement(7125..8471)
                     /gene="miaB"
                     /locus_tag="PLZ74_06050"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOR97920.1"
                     /translation="MRFLLHTYGCQMNVRDSDAAAAVLKAAGHTLVDREADADLILVN
                     TCSVRGKAEDKALGKLGLLCAGKREQPERIVGVMGCMAQRLGAEIFTRVRGLDFAVGT
                     RRAGAIPRLVARVLAGETGLGEWSAPDELPDVPDSHAESGFSALVTILLGCNRRCAYC
                     IVPDVRGTEYSRPAREILAEIDALTRNGVREITLLGQSVMNYGRVHTVWNAADAPSPG
                     GFREPFARLLEAVAAVPGIARVRFTSSHPSGVTDELIRVMRHVPQLCHHLHLPVQSGS
                     DRILKAMRRGYTRAGYLDAVARLRSAMPDLALTTDVIVGFPGETVADFEETRSLMREA
                     AFDNAFIFKYSPRPGTPAAALEDDVAEEEKMRRNQVLLADQDLRGVALNRQLVGTIQE
                     VLAEGPSLRNTARWSGRTPGNKIVIFDPPADLAAGDAVRVRITRAAPQTLYGDPLP"
     gene            complement(8475..10370)
                     /locus_tag="PLZ74_06055"
     CDS             complement(8475..10370)
                     /locus_tag="PLZ74_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOR97921.1"
                     /translation="MWRLLSILGRVCRILFWLALILVLAGILVLYLIGHDIPAPLVQR
                     LAQAVSTDDVHVRIGRATFTLDKGLRLLHVKALPKRIADGPLLAVEEVAVALSLLPQT
                     PMQARIRGVTLKNLTFPDLPPPMSDPPHSLPELPDLAPFPLRIEQADVLGLRVRSLVA
                     NVDVDTNRVAVSEALIHWPDNVFGMTVTGQATLDLTRRHVSGLVAGKAFPDNLMPLFE
                     MLHARGAIRQINCFSKIARPVEAVYAFDVDIDSTDFAMRLGLDVGPCAYRGVPVSYAK
                     GTLFLYGTNIHTTVVIDPLEAQTADGAPITGSLAYREETEGLEIDARTVMHLPPFTTI
                     INVLNRGELDRIRCDTPPSLSVRGAIALSSQESTITNDLRGRVALDKGTILNFGVRDL
                     TADFDMQGYSARFHQAAARSASGGQVNGEVTFTFPEYAATATVFSADIRFTGVELAEI
                     SQAFNVTNERVGLVNGNLHLTGPAYGDTVAGLNGSGHANIRNGVIHRMRLFAGLTDYL
                     TRNIPGISSLVTQSSSSMDFTLRNGVFATENLLLEGDLVSIRGRGTYDLNTDKLDFTV
                     RVSIFRQKTIAGRIVRLMTLPFSRLLLEFRVFGTLEQPEWTYVNLIERITEGFSDAPG
                     TVSQPKP"
     gene            10904..11164
                     /locus_tag="PLZ74_06060"
     CDS             10904..11164
                     /locus_tag="PLZ74_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOR97922.1"
                     /translation="MNVEFDPDKSAANLKKQGTGFLEAQALWEDADRLLVPARTQGGP
                     RHMLVGKTGQKHWSAIFTYRGEAVRIISVRRARKEEVDAYEN"
     gene            11154..11384
                     /locus_tag="PLZ74_06065"
     CDS             11154..11384
                     /locus_tag="PLZ74_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011743982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOR97923.1"
                     /translation="MKTEVLDKAFDNGDDVSKYLNFSKATRPGRELKRVNVDFPMWMV
                     QSLDKEAQRLGVPRQAVIKVWIAERLRVRTHA"
BASE COUNT         2176 a         3390 c         3749 g         2169 t
ORIGIN      
        1 taactcctaa ctcctaaatc cacgcgtagc gcggaagacg cccgcgctcc cgacttgcgc
       61 acacgcaaac cacgcacaaa actcgcatgc ccctgtcacg aaccgattgc tcgatcatag
      121 ctccttctgc cttgtcgttt cgttctgttg ttatggttct gggcagacgt cagcgcagaa
      181 tgacggtcgt gccgagttcc ttgtaaaggt aaaccgcgcc gccctccctg acaacctgcc
      241 agccgggctg cggctcgtcc caggacggaa ggccggtaag aactccgtcg gcctcggcaa
      301 ttttcacttt gtgaagggcg tttttcagcg tcccgaccac aaccagcctg cagccggtaa
      361 acgtcaggtc gcccgtgacc cggagcggat tggggcatcc gccggtttcg tccagcgtga
      421 ccgtataggt gtccgtgagc gtcacgtcac cggtcacctc cccgccgccg gtgagccggg
      481 cgacaaccag cggcgcgccc cgcagattca ggctggcgcc ggccgtgagg gtgacttccg
      541 agcctgcggc gaacacttct gtgttcacgg tatatcccgg ggtgcgccat ttccgcgcga
      601 gataatgctc caactgatcc cgttcggctt tcgtcagcat ccggttgtac gcgatgacct
      661 cgccgatcgc gccgcggaaa cgtcgcgtat cggtggggga gttgacgtag aaacccccga
      721 tggcgttgtt cccggggccg gctttcaccg gcgattcgat gtgcgccgtg accggcgtgt
      781 tcagggcgta atcgaaaccg ctggcggaag ccgggagcgc gatcccgttg tgccacgcct
      841 ccgcgccgac gaaacagttg ccgaccgtcc cgtatgagaa attggtcgaa ctgcctcgga
      901 ctccgaggtc gccgccggag ttgacgcccc aatacccagc catattcatc tggttctgaa
      961 acgcctggaa caccatgacc agcgtctgaa ccgcgatgga tttgttggag cgcatccagt
     1021 tggtggtggc gttcccgaaa aacaccgcgc tctgcccgtt gatgcttccg gtgtaggtgg
     1081 gcggttgcga ggtctccgcg tcccaagcct gcttttcgag gccgaaggcc agtccttcgg
     1141 ttcctgcttt ggaaacccag ttcgtcacat agccgtctgc cgagcgccgt accgttgccg
     1201 cgtccgtcgc gtccagccag tactggaggc cgtcctgcgg cggcagatca ttgaagaccg
     1261 tgaccgccga cgcgccgccg aacgagaccg tccccgcttc cacgcgcagt gccatgtccg
     1321 ccgcgccgca cagcgcgagg ttgaccttct ccccgatggt gccgcggaat ccgttggttc
     1381 ccgtcacggt gagcgtggcg gtctccgcgc cgctgttgat gatgtttccg ccgatgccgg
     1441 acagggtgga gacagtctga tctatgccgt tcaggtcgaa cgccgccccg ggttccacgc
     1501 tcagggttgt cccggccggc agggtcccgt gcacgggtgc gggcgcctcc gcgtgcagtt
     1561 cggtccccat ccacttttgc cacatataat tttcgaccgc cttgatctcg tcccggctca
     1621 gaatccggct gaaggcaatg acctcgccga tgaacccgca gagcgaacgt ccctggtaat
     1681 tgttgccgat cacgttgttt cctgcgccga aatcatcggt gcggaaggcg gagagcagcg
     1741 tcggctcgtt cggcgtgaac gtattgaccc cggcgacgcc gttgatgctc aggtttgcca
     1801 gcgtactgta atggaacacg ctgccttctg tgcccgtgtc gatggaggtg gcgctggcga
     1861 gacgcaaaaa acggctgcca tgatcaccgc gcgtgtttgc ccagaggcag tccatgccgc
     1921 caccggcaac actgtacccg gtcgtctgga agaggatcag caccgtcttg gtggcaaagg
     1981 ccttgttcgc caccaggcag ttggtccaac cgttgaaata gattgcgggc ttggactgga
     2041 tggcgccgcc gacaccgttg gagtagaccg ggcacggcag gccatcccag gtctgtttga
     2101 aggcgacgcc cgttacccgg tccacccagc ccgtcactgc gccggtcgtt tcgtccgttt
     2161 gcacgctggc cgcgtcgttc gcgtcgagat gatacgcgat gtccacctcg cacgggatca
     2221 gctcccggta cagcttgagc gtcccctccc gaaccaccgt cggaccggaa taactctgtg
     2281 gtttggcgat cccgagcgtg ccggaaccga ttttggtcag gccgatgagg ttggtgccgt
     2341 tgtcgcgaag gtaccctccg tataggatgt ccgacgacgt tccgatctcc gccatggccg
     2401 gcgcgtccgc gccttcggcg atcggcgtga tcgcgcgttc ggaactctgg gccagattgc
     2461 ccagtcgggt cgcggcaccg gcgattccga tatccccttg tgtcgtgagc ccgcccatcg
     2521 tcaccgaagg gctggcgaaa agaagtgtgt tcgtcccgct gttgatccag gatccgacac
     2581 cggaaaccgc gttggagata acgaaatccg cattcttgtt gaccgtgatc gtggtgttgt
     2641 tgatcacctc gcctgcgccg aacgtaccgt tggctccgag cgtgagcgtg ccggtctcca
     2701 gccgggtcac gccgagatag gtgttggtgc tgttcagagt taatttattg gaatcctgca
     2761 ggatgacgag tccgccgggg ccggtcaccg gacagtcgaa ttgcaggttt ttgttcatcc
     2821 ggattccggc gtcgtccgcc agcaccaccc gggcgatgtt cgtcgaaaag tcctcgccgg
     2881 tgccggtgaa cacggcccgc ccgtggtaag tgctgatgcc gaatccggca aggctcagtt
     2941 cgctgatcgc tgtcttggta tagttcagga attgggcgcc gcagaggcgg tcgccgaaga
     3001 cgcggaccgc gggtgcggaa gtgaggtccg cgtaaagctg cacccggccc cagtaggccc
     3061 ggatgtcacc gctgttggca taggtgccac tcaatatggt gttgacgtaa tccacattgt
     3121 tgcgcccggt gaacgtgacg cccgccgtgc cgcggatttc ggtatcgatc gtctgtagtg
     3181 ttttgctgtt gttgtcctgt acccagacga tgccttcgga gccgcggaaa tcgatcgcgc
     3241 cgccgctgat gcaggtcttg gtggtcgtga acccttgcag gatgatgccg gcgggcgacg
     3301 tgccgtcgcc gatcgtcagc gtcacgccgg ggttgagggt cagcaccgcg ttaccgtcga
     3361 tgaccagcgc gtggatcgcc gtgtcggcgg aaagcgtggt gttggcggtg atgcgcacga
     3421 tcgcattcga gtccgctccg gcgaacccgt catggtagag gtccgtggcg ggcacgagcc
     3481 cgttggacgc gtcataccgc gcgaagcaca ccggcgtgct gacggtgttc gggccgccga
     3541 gggcgatgac cggcgagacc atgcccgagc ccgcgtggac cagcgcggcc gggtttgtca
     3601 ggaagaactt ctctgaagtg ccgagcgtgc ccgtgccgcc gttgggggcc agatacagcg
     3661 tgccgggaaa aacatcctcg cggacgacct cgccgattgt gagggaagcg gaggcgttgg
     3721 agcctttttt caggctgaag cgcaccgaac ccttgagcgc gaccgcgctg tttgtggcga
     3781 tcgtgagtgc ggcggcggaa ccctcggccg caacgggttc aaacctgatt tcgttgcctt
     3841 cgaacgtgac gcggccgtcg gtgagcgcgg tgccgctttt cgcataaacc gcgaccaagc
     3901 cgtgcttgaa cgtcacgttg gtgaatccgc tgtagaacgc gccgtcatat acgcggagcg
     3961 ttccgcggcc cgttttctcg aacgcgttgt tgttaccgag gaacgggatt ttctggtcga
     4021 cgtacttgcc ctcctcgtct ttcggacgca gatacggcgc atccccgacg aaggtgatcg
     4081 gctggttgcc ggccagcaga aacgcgccgg aatacggaaa cgccataccg gaaatctcaa
     4141 ggccggccac atcctgatac acgccggcgg aaacccattg actcatgctg tcggacgccg
     4201 aggcgtgggt gaacacgccc ccgttctgcg cgggcacgcc atcaagccaa tacttggtgt
     4261 tgctccattt gaggctgtcg ggggcgtatg tcatctggtt gacgaaccag tatccgtccg
     4321 aggccgaaac cgccgccgcg agagcggccg tcgtgatgac cgtgatgagt tgcttcatgt
     4381 gaaccactcc tttgcataca caggtgaacc tctgccatcc cacaggttgc cccggcaccg
     4441 tttcggatcg tatgaccggc atatatacgg cgagggagaa accatcccaa gtgccttata
     4501 atttactcgt ttccgcacgc gtttgtccag aacatccaat gtgggatagg taatcggttt
     4561 gtgacagggg ggtgcgaagg caatgcgttt ctggtgaaaa ggggggcagt gagtcgcgag
     4621 cgcgggcatc cccgcccgcg ttcccgactt tctgtccgtt ccgcccattt tcatttcatg
     4681 aagttttatg tgtttcatgg tccgaaaact tgaggtcggc gttcgatccg gtcgagtcgg
     4741 tcttgcggcg ccgatcatcc tgtgagatac tgaacagact ttaacgggac cggaggagtg
     4801 atgggatggt tccgggggaa caacaaggga ggacgcatgc gtttcgcgat gatggggatg
     4861 gtgttggggt tggtttccgg tgtgtgggcc gaggtcacga tccatgtggc gcccaatgcg
     4921 cctgccgggg gggacgggcg gcggcgttcg ccgtacgcga cgctggagca ggctcgggat
     4981 catatccggg cggcgcgtaa ggaggggctg atcgcgcggg atgagccggt cacggtgctg
     5041 ctggagcccg gggtccatac gctgacgcaa tcgtttgaac tcacgcgcga ggatggcggc
     5101 agcgaggccg cgccggtggt ctaccgggcc gcgaagcggg gacgcgcgcg cctgcggggc
     5161 gggctgacgc tgcccgcgga cgcgtttcag ccggtcacga atggggcggt tctgaaccgg
     5221 ctcgaccccg ccgtgcgcga gcatatccgg gtgtgcgatc tcacgtcgcg cgttcccggc
     5281 gggtttgaac cgttcaagaa cgcttattcg ggaacgccgg ccgcgccgtg gctgtatcac
     5341 aaccgcaagc cgttgacgct ggcgcgctgg cccaacgcgg acgccccgca gggagggtgg
     5401 gcgggtttca ccaaggcggt ggaaacgggc aagccgattc cggattcccc ggatcccgct
     5461 ctgcgaaagc cgcgcccggg ctccttcgag ttcgcggatc cgcggccggc ccgctggaat
     5521 ctggaagagg gggtttggct gatggggtat tggacgcacg actggtcggg cgaggtgatc
     5581 cgcatcgcgt cgtacgatcc ggacaagaaa gtgatcgccc tggcggcccc gcacgggtac
     5641 gggatcgccg cgggaacctg gggcagtgcc gtgcggcgct ttttcgcgct gaatctcatg
     5701 gaagagttgg acgcgccggg cgaatggtat ctggatcgga caagtaagca gctttacgtt
     5761 tatccggagg gggagacgga ggaggggggc gaatgggtct tggcgacgct tacgcagccc
     5821 atgttggctg tctccgacgc cgcgcatatc cgggtgcttg atctggcgtt tgaatattcg
     5881 cactgcgcgg gcgtggtgat catattgaaa aacagcgaac aggtggaagt cgcgggatgc
     5941 gtggtggcga atcacgccgg gagcggcatc agcgtctccg gcagggccaa cacggtgcgg
     6001 tcttgcgatc tgtaccaact cggcaaagcg ggcctctccc tcaacggagg cgaccgcaaa
     6061 aagctgatcg ccgcgaagaa tctggcggtc aacaaccaca tccaccacta cgggctcttc
     6121 cagcggacct atgcggcggg gattgcgctg ggcggctgcg gacagatcgc gcgcaacaac
     6181 tgcattcacg atgcgccgca caacgccgtc ctctacggcg ggaacgagca cctgctggag
     6241 ttgaatgagg tctaccgcgt ggtgatggag acgggggatg cgggcgcgtt ttacacggga
     6301 cgcgactgga ccagccaggg gaatgtgctg cggcacaatt tcgtccacac gctgggcagg
     6361 ggcgatgcga aacatgtcaa caccatgggt ttttatctcg acgattgcga ctgcggcgac
     6421 cggatcgagg gcaatgtgtt ctggcgcgcg ggccgggcga tcatgatcgg cggggggcgc
     6481 gacaatccgg tgatcaacaa tctgatcgtg gagtgcccga tcgggctcca catcgattca
     6541 cgcggaatga cctggaagta ctggaacaac accaacgacc cgaactggcg cttggatgcc
     6601 aaagcccaag agttcgacta ccggaacccg ccgtggagcg tgcgctatcc gaaactcgct
     6661 tcgatcatgg atgagtgtcc ccgcgaaccg ctcaacaacc cggcccgccg caacgtgttt
     6721 gtggattgcg tcgacacctg ctcgttcggc ggcaaggtga tggacctgct gcacaagatg
     6781 gagcttgagg acaacctgat cgtcaacacg gtcggcacga accgggtggg gaaactgcgt
     6841 gaggggatca acaaggggtt ccggacggtt tcgggaacgc cggacaagcc gttcgatccg
     6901 ggattcgagg atgcgccgaa cgggaatttc gcgttgcgcc ggaacgcccg cctctttacg
     6961 gaactgcccg ccttcgaacg catcccgttc gagcggatcg ggctgtacac cgacaccttc
     7021 cggcgtaagc tgccgcgccg gtaagcgcgc gtttcacccc ggcctgcggg ataaccgcag
     7081 gccagagaga gattcgatcg tgcaggtgtg cgtttggccg gcactcatgg cagcgggtca
     7141 ccgtacaggg tttgcggtgc cgcacgggtg atgcgcacac gcaccgcatc gcccgccgcc
     7201 agatcggcgg ggggatcgaa gatcacgatc ttgttgcccg gcgttcggcc cgaccaccgc
     7261 gcggtattcc gcagactggg gccttcggcc agcacctcct gtatggtgcc gacgagttga
     7321 cggttgagtg ccacgccacg cagatcctga tccgccagca acacctgatt gcgccgcatc
     7381 ttctcctcct ccgcgacatc atcttccagt gcggcggccg gggtgccggg acgcggcgag
     7441 tatttgaaga tgaaggcatt gtcaaacgcc gcctcccgca tgagcgagcg ggtctcttca
     7501 aaatcagcca cggtctcacc tgggaacccg acgatcacgt cggtcgtgag cgcaaggtcc
     7561 ggcatggcgg agcgcagacg ggccaccgcg tccagatatc cggcccgcgt gtacccgcgg
     7621 cgcatggctt taagaatgcg gtcggaaccg gactggacgg gcaggtgcag atggtgacag
     7681 agttgcggca cgtggcgcat gacgcggatg agttcatccg tgacgccgga cgggtgactc
     7741 gatgtgaaac ggacgcgggc gatgccgggc acggcggcaa ccgcctccag cagacgggcg
     7801 aacggctcgc ggaagcctcc cggcgaggga gcgtccgccg cattccagac cgtgtggaca
     7861 cggccgtagt tcatgacgct ctgtcccagt aacgtgattt cgcggacgcc gttgcgcgtg
     7921 agcgcgtcga tctccgccag aatctcgcgg gcggggcggc tgtactcggt gccgcgcaca
     7981 tccgggacga tgcagtaggc gcagcgccgg ttgcagccga gcagaatggt caccagcgct
     8041 gagaagccgg actccgcatg gctgtccggc acgtccggca gttcgtccgg cgcggaccat
     8101 tcacccagtc cggtctcgcc ggccagcaca cgggccacca aacgggggat cgcgccggca
     8161 cggcgggtgc ccaccgcaaa atccagaccg cgcacacgcg tgaagatctc cgctcccaaa
     8221 cgctgcgcca tgcagcccat cacgcccacg atccgttctg gctgttcgcg tttccccgcg
     8281 cagagcagac cgagtttgcc gagggccttg tcctccgcct tgccgcggac actgcaagtg
     8341 ttgaccagga tgaggtcggc atctgcctcg cggtcaacca gcgtgtggcc tgcggctttc
     8401 aggaccgcgg cagcggcgtc ggaatcgcgc acgttcattt ggcagccgta ggtgtggagc
     8461 agaaaacgca tgggtcaggg tttgggttgc gaaacggttc cgggcgcatc cgaaaagcct
     8521 tccgtgatcc gttcgatgag gttgacgtaa gtccattccg gttgttcaag cgtgccgaac
     8581 acccggaact ccaacaacag ccgcgagaac ggcagggtca tcagtcggac aatgcgtccg
     8641 gcaatcgtct tttggcggaa gatgctgacg cgcaccgtga agtccagttt gtccgtgttg
     8701 aggtcatagg tgccgcgtcc ccggatgctg acgagatctc cctccagcag gaggttctcg
     8761 gtggcaaaga cgccgttgcg gagggtgaaa tccatagagc ttgatgattg ggtgacgagc
     8821 gaggagatgc cgggaatgtt gcgcgtgagg taatcggtca accccgcgaa cagccgcatg
     8881 cggtggatca cgccgttacg gatattcgca tggccgctgc cgttcaatcc cgcgacggtg
     8941 tccccgtagg ccggtccggt gagatggagg ttcccgttga ccagaccgac gcgttcgttg
     9001 gtcacattaa aggcttgcga aatttcggca agctccactc cggtaaagcg gatatcggca
     9061 gagaagacgg tggcggtggc ggcatactcg gggaacgtga aggtgacttc gccgttgacc
     9121 tggcctccgc ttgcggaacg cgccgcagcc tgatggaaac gtgccgaata cccctgcatg
     9181 tcgaagtcgg cggtcaggtc acggactccg aagttcaaaa tcgttccctt gtcgagcgcg
     9241 actcggccgc ggagatcgtt ggtgatcgta ctctcctgtg atgagagggc gattgctccg
     9301 cggaccgaga gggagggggg ggtgtcgcag cggatccggt ccagttctcc ccggttaagc
     9361 acattgatga tcgtggtgaa ggggggcaga tgcatcacgg ttcgggcatc gatctccaac
     9421 ccttcggtct cctctcgata ggcgaggctt cccgtgattg gcgcgccgtc cgcggtttgc
     9481 gcctccagcg gatcgatcac cacggtggtg tggatgttgg tgccgtacag gaagagggtt
     9541 cctttggcat aggagaccgg cacgccgcga tacgcgcagg ggccgacgtc caaacccagc
     9601 cgcatggcga agtccgtgga atcaatatcc acgtcaaacg catacacggc ctcgaccggc
     9661 cgcgcgatct tggagaagca gttgatctga cggatcgcgc cccgcgcgtg caacatctcg
     9721 aaaagcggca tgaggttgtc ggggaacgcc ttgcccgcga ccaaaccgct gacgtgacgg
     9781 cgggtgaggt caagcgtggc ctgtccggtg acggtcatgc cgaacacgtt gtcgggccag
     9841 tgaatcagcg cctccgacac cgcgacccgg ttcgtgtcca catccacgtt ggccacgagg
     9901 ctccggacac ggaggcccag cacatcggct tgctcgatgc gcagcggaaa cggggccaga
     9961 tcgggcaact ccggcagcga atgcgggggg tcggacatcg ggggcggtaa atccgggaaa
    10021 gtcaggtttt tcagcgtaac gccacgtatg cgcgcctgca tgggagtctg cggcaacagc
    10081 gagagcgcca ccgcgacttc ctctacggcg agcagcggac cgtccgcgat gcgcttgggc
    10141 agagccttca catggagcaa ccgcagccct ttgtccaggg tgaaggtggc gcgcccgatc
    10201 cgcacatgca cgtcatccgt cgaaaccgcc tgcgcgagtc gttgcaccaa cggcgcgggg
    10261 atgtcatggc cgatcaggta cagcacgaga atcccggcca gcacgagaat cagcgccaac
    10321 cagaagagaa tcctgcacac tctgcctaga atagagagga gtcgccacat gatgagaatt
    10381 caggccggac cggacacacc cgatcccaag acaaagcata ccggaagccc tcggatagcc
    10441 gcaacaagaa aggggggaac gcccaacgcg cgttgcctca caattcatga caccggaacc
    10501 aaaaaggttc ggggagggga cgttacggct ggcggctgag gtgcccgtgg gaaccccttt
    10561 tgccctttga ccagaatacg ttggaaacga aagaaaaaat caaggcaggt tctgggtttt
    10621 agatctcgtt ggtcatacgg atggctgccc acttctcatg atatgtgggc agaagagaat
    10681 cgaaagctga atcagcgtaa aggtggagag agggaatttg ggtttccaga aactcccgtg
    10741 ccagactgga aaaaaaggag ggactattcc tagcaacgat gatgcactga acatattcgc
    10801 ctttggctca ggggaccgga gatcgctgac gattcgagaa ggaatcaaaa ccgacgacaa
    10861 agccgactga catccggtat cgttttggta tataatatat accgtgaatg ttgagttcga
    10921 tccagacaag agcgcggcaa acttgaagaa gcagggcacc ggctttctcg aagcacaggc
    10981 gctttgggaa gatgccgaca gacttcttgt gcccgcgaga acgcagggtg ggccgagaca
    11041 catgctggtc ggcaagacgg ggcagaaaca ttggtcagcc atattcactt acagaggcga
    11101 ggcggtccgg atcatatcgg ttcgccgtgc gcgaaaagaa gaggtggacg cctatgaaaa
    11161 ctgaagtctt ggataaggcc tttgacaacg gcgacgacgt gtcaaagtat ctgaattttt
    11221 ccaaggcaac ccgccccgga agagagctga agcgagtcaa cgttgatttt cccatgtgga
    11281 tggttcaatc tctcgacaag gaagcccaac ggctcggtgt tccccggcag gccgtcatta
    11341 aggtgtggat cgctgaacgg ctgagagtca ggactcacgc ttgaaatcgt ccgaacccgt
    11401 cggagcagag acacggggaa aagtgcgtta ggtaagatga gaaggctgtt ctccttgtga
    11461 aaggtttctg gcataatgcc tgaa
//
LOCUS       DAOSRB010000085        11341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5645_length_11341_cov_7.543328, whole genome shotgun sequence.
ACCESSION   DAOSRB010000085 DAOSRB010000000
VERSION     DAOSRB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5645_length_11341_cov_7.543328"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..838
                     /locus_tag="PLZ74_06070"
     CDS             119..838
                     /locus_tag="PLZ74_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOR97924.1"
                     /translation="MRRWVWLGALAIGFGLAAAVGVRGLRDTRPVLVCLGDSLTSCGG
                     QGGRYSDWLARFMLYVRVIDAGVGGDTLMGGRARFERDVLVHNPDILVIALGANDFWQ
                     RNRPPEALAADLEAMVREARSRSMQVVVASCFGDRFFWEEPCVEFTPDRFALAREIAR
                     LERRVCETHGAEYVPNMQIDIKPNRLPPYWDKTDHPNRAGNEQVALRLLPAIRRAVEL
                     DGAKVSPYSEKANSQQGYDKK"
     gene            848..2260
                     /locus_tag="PLZ74_06075"
     CDS             848..2260
                     /locus_tag="PLZ74_06075"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR97925.1"
                     /translation="MIGENEKAEQVAQQAQNFYNKGVAAFERANLDIAIDLLMQCLIL
                     APGFSRARKFLRAAQIAKFRKAKKNPLSSKIQEVAAFFQRLKVTGLMKAGKHEAALIE
                     CEKLLIMNPLHSENVELAVEVAEAAGHPEAALFTVEAAYDNNPDDMTLLRRVADYYMA
                     VGDYDKAREAYLKLNAYKPNDQTIFKLLKDAEARMTMVSGGWEEVAGQKDGYRNLIAN
                     KEQAKKLDIQAKAVVAGTDAESLIEEARGRIEKEPNNLNLYRALARLYTQNKRFDEAV
                     QTLEAAREVNPADPELDRALTATRISAFEARIEALQTAGDAEAAAAAETEMNQFIFDD
                     LASRVQRYPNDLKLRFELGLQYYKYDYFDEAIGQLQLAQRSPKERVEALYYLAKCFAK
                     KGQRDMAIMQLETANDQLPIMDDLKKRVVFELGVLAEESGDIEKAFGYYKDVYGADIG
                     FEDIGARMERIYKLRQSQQG"
     gene            complement(2268..3092)
                     /locus_tag="PLZ74_06080"
     CDS             complement(2268..3092)
                     /locus_tag="PLZ74_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR97926.1"
                     /translation="MLIDVHTHVFPDHLAARALATLTAKTGNAIAPVADGTLSALLEN
                     LQRDGVDRAVVCPIATRPDLFEGLLREALALRDGERGKDAARRLIPFASVHPDDEARF
                     AHLTRVAESGLIGIKLHPYYQPCVLDAPPMLEFFRCCRDLGLVVQCHCGFDIGFPFEP
                     VCGPARVARVMREVPGLRFIASHLGGWRQWEESVTHLLGTDIYLDTAVLRQDREDPLL
                     LRLLREHPTDRLLFATDWPWLGFADGIAFVRSAALGAKAERAILGGNAIRLLGIPA"
     gene            3225..4976
                     /locus_tag="PLZ74_06085"
     CDS             3225..4976
                     /locus_tag="PLZ74_06085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR97927.1"
                     /translation="MHTEHPSGQRGRRPRKPFRRRNRHPGLAEREFKPTQVPKPKGTI
                     PEAFAGTVFGLVHPLLQHAVADEAYLTPTPIQEKAIPHLLDGRDLMGCAQTGTGKTAA
                     FLLPILHRLNLNPKPVERNRPRALVLAPTRELAAQIATSTATYGRYLGLPFAVVFGGV
                     SQYPQVNALNRGAVLVVATPGRLLDLHGQGHIALDGIEEFVLDEADRMLDMGFMPDIK
                     RILAMLPEARHTQFFSATFSPEITRLAMSMVKDPVQITISPDQPTVDRINQTLMFVDK
                     ANKDALLGEILATRPEMSKVLVFARTRHGADKIERKLCRAEISARAIHSDKTQRARMD
                     ALDAFRHGRVRVLVATDIASRGIDVDGITHVVNFDLPEETESYVHRIGRTARAGAAGV
                     ALSFVSAEERGLLRAIERLIRKSIEVDNTHSYHSERAEKAVGKGRDSLPRPAWASRIR
                     TQGGAKGRGAYSRDRGPGRGPQGRRQPDFRRGPGAKPIPSDAPDRRPARAAQSREKPA
                     VETERRDRRRPEGKRFPAAGPAAPRPARGGDEAKKDQGRKPRGGGSGGFKSGGFKPRS
                     RKGGKPKHRPARPPWMT"
     gene            5220..7286
                     /locus_tag="PLZ74_06090"
     CDS             5220..7286
                     /locus_tag="PLZ74_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOR97928.1"
                     /translation="MSTSTQNFLQRAFADGHLQLITSGKTEKIRYATVNVSEKWSDPE
                     EKVRAAYYAELIYRYGYAPEYIGVEVTVPDRTPVDRADLVVFRDKEHTKPFGVIECKR
                     DGISDAAFRQAVEQAFGNGHAHKFRAEYIGVIAGQTRAFYDCSDKFGVLEREANIVAD
                     LPIQYGKPEEFKYYKDAFPKPDISAVSRDDLIKTIRKCHQTLWGGGRLSPPMAFGELT
                     KLIFLKTRDEKTPRKKGEPYKFQIKTNETAQRLATRIKDMYAIERQKEPDVFNDDIKI
                     DDVTLKNVVLHLEGINLTATDLDTKGVAFEQFMDGFFKGDFGQYFTPRNIIALTIQML
                     DIRADDFVIDPACGSGGFLLHALDHVRRQACQYYEADSKEHFDYWHSFAEKRLFGIEI
                     NEEIARVAKMNMIVHDDGHTNVIGNDALEPLINIGRKNASFAAIMGLNLQTGKRDESK
                     GFSKIPTNPPFGAVIKEELHSYLPSYELSRYISKSGGKDEDDAENTDADYKAGKKAVK
                     QRSSVKTEIIYCERIWQLLKPGGRAGVVLPDGLLTNASLQGVRDWILERFKVLAVVSL
                     PQFAFAHFGAGVKSSVVFLEKRQPNVPASDGEAIFMAIAENVGYDATGRSTYQVEMVD
                     EKPGLLRTERQRCDLFDWEVTFNWVPGEGKSPGHWSEQHRHVVPGSGLLGQYAAFKRN
                     PDPFFV"
     gene            7322..8671
                     /locus_tag="PLZ74_06095"
     CDS             7322..8671
                     /locus_tag="PLZ74_06095"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOR97929.1"
                     /translation="MRRGELTGRWDTNFLLNRTDFSQFRYEIIPIENICLDVFQGITI
                     KKTDDVDAPKVLKVKNICLNGSIDFCEIESTTNVPDRKLLKVGDVISPFIGEAIRQHK
                     VALYEDEANKYTVDGNVGVYRPNKKKIKPRFLKAILSSSFVTKQVNQLIGGGGVPFLG
                     ARNAKKLKIPLPPLDIQRRLVAELDAARAESDRALEEAERLIVTFDDYLLNELELTMP
                     DENTQKAFAVRLDAVRGSRIDPSSYQPILARGAPPKVPIKALSELAIVNGNSIEKPDD
                     IETLVPYVGLPECDQTEVREVVMRPYREVRGRNVVRLGDILFARIEPSIFNKKYVLVD
                     DLKGHEYAYTSTEFYVVSHRPGISVTEYIYAMLFCSFVYAQTQGKTTGSSGRRRLAPD
                     HFRLLKIPAPELSMQQSIATEAIRRREESRRLRTKAETIWREARERFEQQLLQGGTP"
     gene            8828..9052
                     /locus_tag="PLZ74_06100"
                     /pseudo
     CDS             8828..9052
                     /locus_tag="PLZ74_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
     gene            9265..11292
                     /locus_tag="PLZ74_06105"
     CDS             9265..11292
                     /locus_tag="PLZ74_06105"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR97930.1"
                     /translation="MSETPVSNVRNFVILGHSGSGKTTLTDAVAFKLGLNDRMGLVTN
                     GSSVSDTTEEEKNRKITIFSSPFTASHKLGDAEYKMVFIDSPGFMDFCGQIQGAIRAA
                     DFALITVDAASGVQVGTRRAWKYCKDGGLASVAFVITGLDKDNTDFAKTVAAIRDTFG
                     VNCVPVTAPAGADAVINLLDASDLPTPLQEIRDSLAESAAETDEALMEKYFEQGSLDP
                     VDIRKGLVTGIQSGGTNPIYSVYPLKNVGVTEMLDSICRLLPAPGTRVYRDTEGNELA
                     PDPDAPAVAQVWRTAVDPFLGQLSYVRVISGTLTTGTSVQNTSTDTKESISSMIYVVG
                     KKQTPVTKAGPGDIVAIPKFKNTKTGNTLAAAGSTVRMPDLDLPPPVMFMAVEAKTQA
                     DEDKLSTAIHRLLDSDPTLHYEKQIETKQALLKGLGDVHLDVAVALMKSQTNVNVNLS
                     TPKVPYRESITAAGEGHYRHKKQSGGAGQFGEVYLRIFPRREDEEGEWFVDKTVGGSI
                     PGNFIPAVLKGVEEGKLAGAVAGYLVQNVNVEVYDGSYHPVDSKEIAFKIAGMRAFRD
                     AMTKAKPVLLEPIMSLKITIPEAFMGAISGDMPNKRGRVLGMEVEEGIQVITAEAPLA
                     ELFKYAAELRSITGGQGSFTLEFDRYDIVPSNVAQKVIAEAAKNRKDDDED"
BASE COUNT         2465 a         3249 c         3450 g         2177 t
ORIGIN      
        1 catcctcacg cccttgcctc ggggacccgc atccattacg gttgggcttt cttccgcgtg
       61 tgtgatgtgg tacaacacac cccatgcgga acggaaaggg taaaacggtg ggggagcgat
      121 gaggcgatgg gtttggctgg gcgcgttggc aatcgggttc gggctggcgg cggcggtagg
      181 ggttcggggg ttgcgggaca cccggccggt gctcgtttgc ctgggggaca gtctgacttc
      241 ctgcggcggg cagggcggtc gctacagcga ttggttggca cggtttatgc tgtatgtccg
      301 ggtgattgac gctggtgtgg gcggagacac gctcatgggc ggaagggcgc gtttcgagcg
      361 ggatgtgttg gtgcacaatc ccgatatcct ggtgatcgcg ctgggcgcga acgatttctg
      421 gcagcgcaac cgtccgccgg aggcgttggc cgcggatttg gaggcgatgg tgcgcgaagc
      481 gcgttcgcgc tcgatgcagg tggtggtggc gagctgtttc ggagaccggt ttttctggga
      541 ggagccgtgc gtggagttca cgccggaccg gttcgcgctg gcgcgcgaga tcgcgcggtt
      601 ggagcgccgg gtgtgcgaga cgcatggggc ggagtatgtg cccaacatgc agatcgacat
      661 caagcccaac cggcttccgc cctactggga caaaaccgac caccccaacc gcgccggcaa
      721 tgagcaggtg gcgttgcggc tgctgcccgc aatccggcgg gcggtcgaac ttgacggcgc
      781 caaggtttcg ccgtatagtg aaaaggctaa ttcccagcag gggtatgaca agaagtgagg
      841 acgcggcatg ataggtgaaa atgagaaagc ggagcaggtg gcccagcagg cccagaactt
      901 ctacaacaaa ggggttgccg ccttcgagcg cgccaacctt gatatcgcga ttgaccttct
      961 gatgcagtgc ctgatcttgg cgccgggttt ttcacgggcc cggaaatttc tgcgggccgc
     1021 gcagatcgcc aaattccgta aagccaagaa gaatccgctt tccagtaaga tacaggaggt
     1081 ggcggccttt ttccagcggc tgaaggtcac ggggctgatg aaggccggca agcatgaggc
     1141 ggcgctgatc gagtgcgaga agctgctcat catgaatccg ctccactcgg agaatgtgga
     1201 gctggcggtg gaggtagccg aggccgccgg acacccggaa gcggcgctct tcacggtcga
     1261 ggcagcctat gataacaacc ccgacgacat gacgctgctg cggcgcgtgg ccgattacta
     1321 tatggcggtg ggcgattatg acaaggcgcg tgaggcgtat ctgaagctga acgcctacaa
     1381 gccgaacgac cagacgattt tcaaactcct caaggatgcg gaagcccgta tgaccatggt
     1441 cagcggcgga tgggaggagg ttgccggaca gaaggacgga taccggaatc tgatcgcgaa
     1501 caaggagcag gccaagaaac tcgacataca ggccaaggcg gtcgtggcgg gtaccgatgc
     1561 cgaatccctg atcgaggagg cacggggccg gattgagaaa gagccgaaca accttaacct
     1621 ctaccgtgcc ctcgcgcggc tctatacgca gaacaagcgc ttcgacgagg cggtgcagac
     1681 gctggaggcg gcgcgtgagg tgaatccggc ggatccggaa ctggaccggg ccctgaccgc
     1741 cacgcggatt tcggcgttcg aggcgcggat cgaggcgttg cagaccgcag gcgacgccga
     1801 ggccgcggcc gcggccgaga cggagatgaa ccagttcatc tttgatgatc tggcctcgcg
     1861 cgtgcagcgc tacccgaatg atctcaagct gcgtttcgag ctgggcttgc agtattacaa
     1921 gtacgattat ttcgacgagg ccatcggcca gctgcagctc gcgcagcgca gtcccaagga
     1981 gcgtgtcgag gcgctctatt atctggcgaa gtgcttcgcc aaaaagggcc agcgcgacat
     2041 ggcgatcatg cagcttgaaa ccgccaacga ccagctgccg atcatggatg atctcaaaaa
     2101 gagggtcgtt ttcgagttgg gtgtgctggc cgaggagtcg ggggacatcg agaaggcctt
     2161 cgggtactac aaggatgtct acggcgcgga tatcggtttc gaggatatcg gcgcgcgcat
     2221 ggagcgcatc tacaagctgc ggcagtccca gcagggctga gccggctcta cgccggaatc
     2281 cccagcaaac ggatcgcgtt gccgcccagg atggcgcgct cggccttcgc gccgagcgcg
     2341 gccgaccgga cgaaggcgat gccgtccgcg aatcccagcc aaggccagtc ggtggcaaac
     2401 aggagccggt ccgtcggatg ttcgcgcagc aggcggagca gcagcgggtc ctcccgatcc
     2461 tggcgcagga cggcggtgtc cagatagatg tccgtcccga gcaggtgtgt caccgactcc
     2521 tcccattggc gccacccgcc cagatgcgag gcgatgaagc gcaagccggg gacctcccgc
     2581 atcacgcgcg ccacgcgggc gggaccgcag accggttcga acgggaaccc gatgtcaaac
     2641 ccgcagtggc actgcaccac aagccccaga tcgcggcaac agcggaaaaa ctccagcatc
     2701 ggcggcgcat caagcacgca aggctggtag tacgggtgca gtttgattcc gatgaggccg
     2761 ctttcagcca cgcgggtgag atgggcgaaa cgggcttcgt catccggatg caccgaggcg
     2821 aacgggatca ggcggcgggc cgcgtctttg ccgcgttcgc cgtcgcgcag cgccagagcc
     2881 tcccgcaaca gcccctcgaa aagatcgggg cgcgtggcga tcgggcagac cacggcccgg
     2941 tccacgccgt cacgctggag gttttccagc agtgcgctta gggtcccgtc cgccaccggg
     3001 gcgatcgcgt ttccggtttt cgcggtgagc gtggcaaggg cccgcgcggc caaatgatcg
     3061 ggaaagacgt gggtatggac atcaattagc atcagaaccc cccaagccag caggatgcgg
     3121 ccaataacga tgtagaataa tacgggacgg cccgcacggc gagaataaac cgcaggggtc
     3181 ttgactttac ttggacgggc ggaaagagta tatacgaacg ccacatgcat acagaacacc
     3241 catccggaca gcgtggccgc cgtccccgta aacctttccg tcgtcggaat cgtcatcccg
     3301 gcctggcgga acgcgagttc aagccgaccc aggtccccaa acccaagggc acgatcccgg
     3361 aagcttttgc gggcacggtt ttcggtttgg tccatcccct tttgcaacat gccgtggcgg
     3421 atgaggccta tctgaccccc acgccgattc aggaaaaagc catcccgcac cttctggatg
     3481 gccgcgacct catgggctgc gcgcagaccg gcaccggcaa gaccgccgct tttctgctgc
     3541 cgatcctcca ccggctgaat ctcaacccca aaccggttga acgcaaccgc ccccgggcgc
     3601 tcgtgctcgc tccgacgcgg gaactggccg cgcagatcgc caccagtacc gcaacctacg
     3661 gccgttacct cgggctgccg ttcgcagtgg tgttcggcgg ggtcagtcaa tatccgcagg
     3721 tcaacgcgtt gaaccgcggg gccgtgctgg tcgtggcgac gcccggacgg ctgctggacc
     3781 tgcacggaca ggggcacatc gcgctcgacg gcattgaaga gttcgtgctg gatgaggcgg
     3841 accgcatgct ggacatgggg ttcatgccgg acatcaagcg cattctcgcg atgctgcctg
     3901 aagcgcgcca tacgcagttc ttctctgcca ccttctcacc ggagatcacc cggctggcca
     3961 tgtcgatggt gaaagatccg gtgcagatca ccatctcgcc cgatcagccc acggtcgacc
     4021 ggatcaacca gaccctgatg ttcgtggaca aagccaacaa ggacgcgctg ctgggcgaga
     4081 tcctcgccac ccgtccggag atgagcaagg tgctggtttt cgcgcggacg cgtcacggcg
     4141 cggacaaaat cgagcgcaag ctctgccggg cggagatttc ggcgcgcgcg atccacagcg
     4201 acaagaccca gcgggcgcgt atggacgcac tggacgcttt ccgccacggc cgggtgcgcg
     4261 tgctggtcgc gacggatatc gcttcgcgcg gcattgacgt ggacggcatc acgcatgtcg
     4321 tcaattttga tctgccggag gagaccgaat cgtacgtgca tcgcatcggg cgcacggcgc
     4381 gcgctggcgc ggcgggggtt gccctctcgt ttgtcagcgc cgaagagcgc ggtctcttac
     4441 gggccatcga acggctgatc cggaaaagta ttgaggtcga caacacgcac tcctaccatt
     4501 ccgaacgggc ggagaaggcg gttggcaaag gcagggattc cctgccgcgg cccgcttggg
     4561 cgagccgcat ccgcacgcag ggcggcgcga agggccgtgg cgcgtactcg cgcgaccggg
     4621 gtcccggtcg cggccctcaa ggtcggcgcc agccggattt ccgtcgcggt cccggcgcga
     4681 agccgattcc gtccgatgcc ccggaccggc ggcccgcccg cgcggcgcag tcccgcgaaa
     4741 aaccggcggt tgagacggaa cgccgtgacc gccgccgtcc cgagggaaag cgttttcccg
     4801 ctgcgggtcc cgcggctccg cgtccggcac gcggcggcga cgaggcaaag aaggatcagg
     4861 gacggaaacc gcggggtggc ggcagcggag gtttcaagag cggcggcttc aagccccgct
     4921 cccggaaggg cggcaagcct aaacatcgtc ccgcgcgtcc accgtggatg acctgatctg
     4981 ccccattgcg cgtattgggt ttcgggtttc attcgtacgg atccggccac aaatcgcatg
     5041 gcactttttt ctcacacaaa ggcacaaagg gcacaaagtt ttttggggat ggtcaaatta
     5101 cctgtccctt agtcgtcaag aggcgctcga caccgcgcag gaaaccctcg cgggtttaca
     5161 gcatgtatga aaaaaagaag agcagtcttc cggactcaac aaaacaaagg ggaattgaga
     5221 tgagcacgag cactcagaac ttcttacaac gcgctttcgc ggacggccat ctgcaactca
     5281 ttaccagcgg taagaccgag aagatccgct acgctacggt gaatgtttcc gagaaatgga
     5341 gcgatccgga agagaaagta cgagccgctt actatgccga actaatctat cgctacggtt
     5401 atgcgcccga gtacattggt gtcgaggtga ctgttcccga ccgcactccg gtggaccgcg
     5461 ccgatctcgt ggttttccgc gacaaggagc acaccaagcc tttcggcgtc attgagtgca
     5521 agcgcgacgg catttcagat gccgcgttca gacaggcggt ggaacaagct ttcggtaacg
     5581 gtcacgccca taaattccgt gccgagtaca tcggtgtcat cgctggtcaa acccgcgcct
     5641 tctatgactg ttccgataaa tttggtgttc tggagcgcga ggcgaatatc gttgccgacc
     5701 tgccaatcca atatggcaag ccagaggaat tcaaatacta caaggatgcc tttccaaagc
     5761 cagatatcag tgcggtcagc cgtgatgatt tgatcaagac catccgcaag tgccaccaaa
     5821 ccctgtgggg aggtggccgc ctctcgccgc ccatggcctt cggagagttg accaagctaa
     5881 tctttttgaa gactcgagac gaaaaaacac cgcgcaagaa gggtgagcct tacaaatttc
     5941 agatcaagac gaacgaaaca gcccagcgtc tcgccacccg catcaaggac atgtatgcca
     6001 tcgaacggca gaaagaacca gatgttttta acgacgacat caaaatcgat gatgtcaccc
     6061 tgaaaaatgt ggtgctccac ttagagggca tcaacctcac cgccactgac ctcgacacca
     6121 agggcgttgc cttcgagcag ttcatggatg gtttttttaa gggtgacttt ggccagtact
     6181 tcacgccacg caacattatc gccttaacca tccagatgct tgatatccga gccgatgatt
     6241 tcgttataga cccggcttgt ggttccggcg gtttcttgtt gcatgccctc gaccatgtcc
     6301 gcagacaggc ctgtcaatac tacgaagcag atagtaagga gcattttgat tactggcaca
     6361 gttttgccga aaagcggctg tttggcatcg agattaacga agagatcgcc cgcgtcgcca
     6421 agatgaacat gatcgttcac gacgatggcc acaccaacgt catcggcaac gacgctctgg
     6481 aacccttgat taacatcggg cgcaagaacg caagctttgc cgccatcatg gggctcaatc
     6541 ttcaaaccgg gaagcgcgac gagagcaagg gcttctccaa gatcccgacc aatcctcctt
     6601 ttggtgcggt gatcaaggaa gaactgcaca gctaccttcc cagctatgag ctttcccgct
     6661 atatttcaaa gagtggcggc aaggacgaag acgacgcaga aaataccgat gctgattaca
     6721 aggcaggcaa gaaggcggtc aagcagcgct ccagtgtcaa gactgagatc atctactgtg
     6781 agcggatttg gcagcttttg aaaccgggtg gacgggctgg cgtagtcctg cccgatggtc
     6841 tattgaccaa cgctagcctg caaggagtgc gcgactggat tctggaacgc ttcaaggtgc
     6901 tggctgtggt gagcctgccg caattcgcct tcgcccattt tggcgcgggg gtgaagtcca
     6961 gcgtagtttt cttagagaag cgccagccga acgtgcccgc ctctgacggc gaggccatct
     7021 tcatggccat agccgaaaac gtcggatacg atgccactgg ccgcagcacc taccaagtgg
     7081 agatggtgga cgaaaaaccg ggactattgc gcaccgagcg ccagcgctgc gatctgttcg
     7141 actgggaggt gactttcaac tgggtgcccg gcgaaggtaa gagccccggg cactggtccg
     7201 agcagcatcg ccatgtcgtc cccggtagtg ggctactcgg gcaatacgcg gccttcaagc
     7261 ggaatccaga cccttttttc gtctaacccc cgcacccgcg gggcagccag tctgcttcgc
     7321 ggtgcggcgg ggggaattaa ccggacgctg ggacactaat tttttattga accgaacaga
     7381 cttttcacag tttcgttatg aaattattcc gattgaaaat atctgtttgg atgtgtttca
     7441 aggaattact attaaaaaaa cagacgatgt ggatgcacca aaagtgttga aggttaaaaa
     7501 catttgcctt aatggttcta tcgatttttg tgaaattgag tcgactacaa atgttcccga
     7561 cagaaagttg ttgaaagtcg gagatgtgat aagcccattt attggcgaag caattagaca
     7621 acataaagtt gcactgtatg aggatgaagc caacaaatac actgtagatg gaaacgtggg
     7681 tgtatatcgg cctaacaaga aaaaaattaa accacgattt cttaaggcta tattgtcttc
     7741 atcttttgtg accaaacagg tgaaccagct tattggaggt ggtggagttc catttcttgg
     7801 agcgaggaat gcaaaaaaat tgaaaatccc ccttccgccc cttgacatcc agcgccgttt
     7861 agtggctgaa ctcgatgccg cccgcgccga gagcgaccgc gccttggagg aagcggagcg
     7921 gttgatagtt acattcgacg attatctgtt gaatgagctt gaattgacca tgccagacga
     7981 gaatacacaa aaggcatttg cggttcgtct agatgccgtt cgtggaagtc gaatagatcc
     8041 ctcctcctat caacctattc ttgcccgagg ggcacctcca aaggtgccca ttaaggccct
     8101 ttctgagctt gccattgtta acggcaactc tattgagaaa cctgatgata tagaaaccct
     8161 tgtcccctat gttggacttc ctgaatgtga ccaaacagag gtgcgggaag tcgttatgcg
     8221 accctatcgc gaagtgaggg gccgaaacgt tgttcgcctt ggagacattc tttttgcacg
     8281 tattgagccc agcattttca acaaaaaata cgtacttgtt gatgacttaa aggggcacga
     8341 gtatgcgtac acctcaacag aattttatgt tgtaagccat cgccccggca tctccgtgac
     8401 tgaatatatc tacgcgatgt tgttctgttc ttttgtctat gcacagactc aagggaagac
     8461 aacagggtcg tctggaaggc gtcggcttgc cccagaccat ttccgtttat taaaaatccc
     8521 tgctccagaa ctaagcatgc agcaatcaat cgcgactgaa gccatcaggc gacgtgagga
     8581 atcgcgacgc ctccgtacta aggccgaaac catctggcgc gaagcacgcg aacgttttga
     8641 gcaacaacta ctacaaggag gtaccccatg attctctccg gcattctgca ctattctttg
     8701 ggtggcttca cggtaatccg gggcgttgcg ccgcttggag aattggcgcg ttgctcggcg
     8761 attctggaag gcaaacgggt catcgctccg tcccgcgata tacctatcgc cgtaaccccc
     8821 cctcaagtcc ccccaagtcg gcgagctgct ggcggcactc caaggcgaga tggggcgcga
     8881 ggcgctgcaa tccgccctgg ggctttctga ccgcaaatcc ttccgtgagc gttacctcaa
     8941 accggccttg gctgacggtc tgattgagat gaccatcccc gacaagccca acagtcgcct
     9001 gcaaaaatac cgcctgaccg acggggggcg ccagggattg gcagggagat gaggctgtgt
     9061 cacgccgttt tctgattgtt cccgtgcctg attttcgacc ggtgcgcggc gcacggtctt
     9121 ttgggattgc ctcagggtcg tctctttgcc atactctttt ataacacttc tgtccagaat
     9181 gccacgaggg tggggttcgg attcggagat aacatggggc ggataccagc agcggatccg
     9241 cccgcaaaaa atggaatgct ttacatgagt gaaacacctg tgtcaaacgt ccggaatttt
     9301 gtgattctcg ggcatagcgg aagcggaaag acgaccctca ccgatgctgt ggctttcaag
     9361 ctggggctga atgaccggat gggtctggtc accaacggtt ccagcgtttc ggatactacg
     9421 gaagaggaga aaaaccgcaa gatcacgatt ttttcgagtc cgttcaccgc gagtcataaa
     9481 ctgggtgacg ccgagtacaa gatggtgttc atcgattcgc ccggattcat ggatttctgc
     9541 ggccaaatcc aaggcgccat acgtgccgcc gatttcgctc tgatcacggt tgacgccgcc
     9601 tccggggtgc aggtcggcac ccgccgcgca tggaaatact gcaaggatgg cggtctggct
     9661 tcggtcgctt tcgtgatcac cgggctcgac aaggacaaca ccgatttcgc caagaccgtt
     9721 gccgcgatcc gcgacacgtt cggcgtgaac tgcgtgcccg tgactgcgcc cgccggtgcc
     9781 gatgcggtga tcaatctcct tgacgccagc gacctgccga cgccgcttca agagatccgc
     9841 gacagcctgg ctgaatccgc cgctgaaacg gatgaggccc tcatggaaaa gtatttcgag
     9901 cagggttcgc tggatcccgt cgatatccgc aaggggctcg tcaccggtat ccagtccggc
     9961 ggcaccaacc cgatctactc ggtctacccg cttaaaaatg tcggtgtcac agagatgctg
    10021 gacagcatct gccgtctgct gcctgccccc ggtacccgcg tttatcgcga caccgaaggc
    10081 aacgagctgg ctcccgaccc ggacgcgccc gccgtggcgc aggtctggcg tacggcggtt
    10141 gacccgtttc tcggccagct cagctacgtc cgcgtgatct ccggtacgct caccaccggt
    10201 acgagcgtgc agaacacttc caccgacacc aaggagagca tctcctccat gatctatgtg
    10261 gttggtaaga agcagacgcc tgtcaccaag gccggccccg gcgatatcgt ggcgattccc
    10321 aaattcaaaa acaccaagac cggcaacacc ctcgctgccg ccggatcgac ggtgcgcatg
    10381 ccggatctcg acctgccgcc gccggtcatg ttcatggcgg tcgaggccaa gacccaggcc
    10441 gacgaggaca aactctcgac cgcgatccac cgtctgctgg acagcgatcc caccctgcat
    10501 tacgaaaagc agatcgaaac caagcaggcg ctgctgaagg gcctcggcga cgtgcatctt
    10561 gatgtggcgg tcgccttgat gaaatcgcag acgaacgtga atgttaatct gtcgactccc
    10621 aaggtgcctt accgcgagtc catcaccgcc gctggcgaag gccactaccg ccacaaaaag
    10681 cagtcgggcg gtgccggaca gttcggcgaa gtctacctgc gcatcttccc gcgccgcgag
    10741 gacgaggagg gcgaatggtt tgtggacaag accgtgggcg gctcgatccc gggcaacttc
    10801 atccccgccg tactcaaggg cgtggaggag gggaagctgg ccggtgcggt cgccggttac
    10861 ctggtgcaga acgtcaacgt tgaggtctac gacggctcct atcacccggt cgactctaag
    10921 gaaatcgcct ttaagatcgc cggtatgcgc gccttccgtg atgccatgac caaggccaag
    10981 cccgtgctgc ttgagccgat catgagcctg aagatcacga tccccgaggc cttcatgggc
    11041 gcgatcagcg gcgacatgcc caacaagcgc ggtcgcgtgc tcggcatgga ggtcgaggag
    11101 gggatacagg tgatcactgc ggaagccccg ctcgcggagc tgttcaagta cgcggcggag
    11161 ttgcgctcca tcactggagg ccagggttcc ttcacgctgg agtttgaccg ctacgatatc
    11221 gtgccctcca acgtggcgca gaaggtcatt gccgaagccg cgaagaaccg gaaggacgac
    11281 gacgaggact gagcgtatcc aaggagaagt taggagttag gagttaggat ttaggagtta
    11341 g
//
LOCUS       DAOSRB010000086        11227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5708_length_11227_cov_7.983799, whole genome shotgun sequence.
ACCESSION   DAOSRB010000086 DAOSRB010000000
VERSION     DAOSRB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5708_length_11227_cov_7.983799"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLZ74_06110"
     CDS             <1..609
                     /locus_tag="PLZ74_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOR97931.1"
                     /translation="IDLMQTHAQAVEPFKTPIAETMACLMELRKEGKIREIGVSNCTP
                     EQMDEYLAAGVIVSNQPRYSLLDRAIEHELLPYCRAKDLAVLVYSPLEQGLLTGKIGM
                     DTSFDPEAFRNKLPWFALDKRRKVLDMLEGWHDLTDKYGCTLSQLVIAWTVSQPGVTA
                     ALCGARKEANVLENAKAGDLLLEAADRERMRRDAEALAPSEP"
     gene            614..1678
                     /locus_tag="PLZ74_06115"
     CDS             614..1678
                     /locus_tag="PLZ74_06115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOR97932.1"
                     /translation="MRTAVQHDTLSDSGGGVILVSCREKIRDADRGERESAEAGGPLP
                     RVLSVPVPADATGRTDVWLTAQLPEFSRARLQALIREGRVTCRGVAVKANASPQPGSV
                     IEICLPAPVAAEPAPEAIPLDTVYEDRDCLVINKPAGLVVHPAPGHAAGTLVNALLHH
                     CRDLVGVGGVQRPGIVHRLDKDTSGLMVVAKHDAAMAGLVRLFQTGEIRKEYLTLVHG
                     TPPQVSGTLRTRIGRHPVHRKKMAVVETGGKEAVTHYTVEKRLGGLSLVRCRIETGRT
                     HQIRVHMLSLGCPVAGDALYGKSAEDKRLPHVPPRQLLHAARLAFAHPLTGVELDFAA
                     PLPADFLPFLEESEGLRIFV"
     gene            complement(1916..2005)
                     /locus_tag="PLZ74_06120"
     tRNA            complement(1916..2005)
                     /locus_tag="PLZ74_06120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1967..1969),aa:Ser,seq:cga)
     gene            2171..3367
                     /locus_tag="PLZ74_06125"
     CDS             2171..3367
                     /locus_tag="PLZ74_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR97933.1"
                     /translation="MSSGFFDRLVARIDKLGPESLQAQMMRLARERGFLETIFQTIQE
                     GIMVLDSEGKLLYANRAAEKLAGFEFSRTKGRSVLRLLREWDWEHLFDVPEGNSQWNR
                     MVTREVEITYPEHRFVSVYAVPLDEEGVSEKGVLVIFRDVTRDRLQEASTLENERSNA
                     VKLLAAGVAHEIGNPLNALNIHLQLLARELSRVPEETRKPLEDLVEVARAEVGRLDAI
                     ITQFLRALRPSKPVLQTECPKEVLQETLRVLKSEFENRRIQVAVDVPDHLPAMRLDRA
                     QIKQVFFNLIKNALEAMSDGGTLKVVFTVGDVFVDIAFIDSGKGLLPDEFGRVFDPYY
                     TTKAKGNGLGLMIVKRIIEEHGGEIELSSKPGEGTCFKIRLPRSERRIRLLGTPTKEE
                     THEKDR"
     gene            3351..4775
                     /locus_tag="PLZ74_06130"
     CDS             3351..4775
                     /locus_tag="PLZ74_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR97934.1"
                     /translation="MKKTGKPVVLVVDDDKNTRDGLQRALQHHYEVKAVESADAALEL
                     LAGGLQADVMLSDLRMPGMDGLGLLRRVMAQYPRTVCILLTAYGSVETAVDAMKQGAY
                     DFLTKPINLDHLDLLVARAIKSRDLEKKVETLENQLNEKYGMESIIGESPAMRQVFEI
                     IRQTAPTQASVLIQGASGTGKELVAQAIHRLSPRAKGPFVAVHCAALSPTLLESELFG
                     HERGAFTGATVQRKGRFEMADGGTLFLDEVSEIDPSIQVKLLRVLEERTFERVGGEET
                     IETDIRVIAATNRDLRAYVKEGKFREDLFFRLNVVDILLPSLSERTGDIPLLADRFLK
                     EYTERNNRQIDGFTPDAMGLLTAYAWPGNVRELRNAIEKMVVLSRSDRLSARDVPANI
                     REAVRGSASAATRAPALLSGSLADTERRKIMAVLEKNGGNRTRAAEELGISRRTLHRK
                     LREYKAMSSDVSAEDEAKEDWSHA"
     gene            4768..5670
                     /locus_tag="PLZ74_06135"
     CDS             4768..5670
                     /locus_tag="PLZ74_06135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOR97935.1"
                     /translation="MRSPTSFSLPNVEVLEVIGHGGMASVWKARQVSLDRLVAVKILS
                     PDFSSDPEDIRRFRQEAQAAARLKHPGIVQVYDANFSDGVYYFIMELVEGYTLGNLVR
                     RKGRVPMEDALIVAENVAVVLNYAWETAQIIHCDIKPDNIMADADGTVKVTDLGLCRS
                     LTLRKGSTTDKADEILGTPAYMSPEQVYGSDRLDCRSDIYALGVTLYHLVTGRVLFDG
                     QSEDETLRSHTGDAQAPDVRTLAPETSEAFALLLEKMLAKHPDHRHRDWKSVLTDLSR
                     LRSGHLPCPALIPSKGSSMLRLTQ"
     gene            5733..6944
                     /locus_tag="PLZ74_06140"
     CDS             5733..6944
                     /locus_tag="PLZ74_06140"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOR97936.1"
                     /translation="MRKKRLWIVVMMSALAGFAAAGKVGPWERAPLYQTPQVWDAPEY
                     ATNGVKAVFYEGEVYQGKPTRVFAYYALPKVEPGTKVPGIVLIHGGGGSAFIRWVKLW
                     NSRGYAAISMDTCGAVSGNAFGDEQRGHQRHAWAGPAGWGGFDKYADPVRDQWTYHAV
                     AAAVRGHSLLRSLPGVDPARIGVTGISWGGYLTCIVAGVDDRFAFAVPVYGCGFLGDN
                     SCWLTRFQSMKRENAEKWLALWDPSVYLPLAKMPFLWVTGSNDFAYPMDSLQKSYRAL
                     KVPRTLCVRLRMPHGHGAAGENPAEILAFADHITRGASALPEIKRVHREDRKVRITFT
                     GNRRVVVRAELNHTADTGIWKDRKWEAVPFELNAGLEEAALEIPATARVYYINLFTDD
                     GLVVSSEHEEL"
     gene            6953..8185
                     /locus_tag="PLZ74_06145"
     CDS             6953..8185
                     /locus_tag="PLZ74_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR97937.1"
                     /translation="MRRYVIRRVLEMIPTTFGIVLLTFVLFHVVGGSPAEVVLGKNAS
                     AEALAAFDARYGYDKPLVFGQWVRLRAMRDIPRAHLPQADAGGLRRVPLAYALPAGSY
                     RIHAPHVLIGLRESATGMTEDAMIDGPAPLTFAVREGWRVVSLDVGGALPSVIRLRRR
                     TAGLFDSQFMRYLLSLARGDLGVSSEHNLPVARILRNGVGPSLSLTIPILIGGTLVGV
                     MLGLVCAAWRGGVADKAVLILSTVLMSVNYVVWVLAGQFLLAFKLRLFPIWGFESAAY
                     LVLPVLIGIVSGLGRDVRFFRAAILDEVYKPYVRTAQAKGLGGRRIMVRHVLRNSLIP
                     IVTYVSLSIPFLFTGSLMLESFFGIPGLGSVSLNALHSSDMAVVRAVVVLGALMYQGV
                     NLVTDLGYAWLDPRVRLG"
     gene            complement(8447..10843)
                     /locus_tag="PLZ74_06150"
     CDS             complement(8447..10843)
                     /locus_tag="PLZ74_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOR97938.1"
                     /translation="MNPTASRLTRSPRLLRLRALTLAHLVLFLPLVVPAATEGPYTYT
                     VSNGKATITDFDSNYSGDLSITNELGGCPVTAIGDYAFYSCSSLISVTIPDSVINIGS
                     KAFYNCTSLTNAVIGNSVINIGNYAFYNCTALTSVNIPDSVTSISFGAFCSCTALTNA
                     VIGNGVTTIDYGAFRYCASLTSVTIGNSVTNIGKSAFSDCISLNTVIIGNSVMNIGES
                     AFEECISLAYVTIPDSVTTIDYAAFYGCTSLTSVTIGNGVTTIDYGAFRYCSSLTAIN
                     VSEHNLNYASLNGVLFNKTLSTLIQCPVGFSGHFTIPDTVTTIGGYAFYNCTVLTSVT
                     IPDSVTTIDSSAFYGCTSLTSVTIGNGVTNIGEGAFKDCISLAYVTIPDSVTTIGNEA
                     FSFCASLTSVTIPDSVKTIGDEAFSCCASLTTLLFCGNAPNAPTTSYGFDDTPATVFY
                     FPGTSGWGSTFARRPAKQLLTYAEKNGGAVITSSDPSFPGSLSIPEKLAGLTVTSIGP
                     SAFRNCASITSVTIPDSVTSIGPYAFYDCTSLTSANIPDGVTSIGTYAFYNCHSLEEV
                     TLPDSVTTIGDFAFAFCSSLLDVIIPDNVRRIGRGTFSGCYGLESVTLPTGITAIGDD
                     AFSYCASLTAIALPDTLTSIGDYAFTGCESLATVIIPAGVTSVGDDAFSYCGALTTVL
                     FTGDAPDIGTYAFDTTPAALYHLPSAAGWDAASYPVVCWNPAISPASPPRFESGTFAF
                     SLTGNADIPVRIEACDSLTSPSWDTVADIAIPASGTFDFTDPGAATRPARFYRVRFPQ
                     "
     gene            11004..>11227
                     /locus_tag="PLZ74_06155"
     CDS             11004..>11227
                     /locus_tag="PLZ74_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97939.1"
                     /translation="MKRHGSVFLTANCANHANDAKGHGAVFRVLGDDKIWSAVTRAAL
                     CAGCGTAFARYLTTASLLKNAEEPYSLTAI"
BASE COUNT         2190 a         2831 c         3954 g         2252 t
ORIGIN      
        1 attgacctca tgcagacgca cgcgcaggcg gttgagccgt tcaagacgcc gatcgcggag
       61 acgatggcct gtctgatgga gttgcggaag gaggggaaaa tccgtgagat cggcgtctcg
      121 aactgtacgc cggaacagat ggatgagtac ctcgcggccg gtgtcatcgt cagcaaccaa
      181 ccccgctaca gtctgctcga ccgtgccatc gagcacgagc tgttgccgta ttgccgtgcg
      241 aaggatctgg ccgtgctggt ttactcgccg cttgagcagg ggttgctcac cgggaagatc
      301 ggcatggaca cctcgtttga cccggaggcg ttccggaaca agctcccctg gtttgccttg
      361 gacaaacggc gtaaggtgct ggacatgctg gagggctggc atgacctgac cgacaaatac
      421 ggctgcaccc tctcgcaact ggtcatcgcc tggaccgtct cccagcccgg ggtgacggct
      481 gccttgtgcg gtgcccgcaa ggaggcgaac gtcttggaga acgcgaaagc gggtgatctg
      541 cttttggagg ctgcggatcg ggaacggatg cggcgggatg ccgaggcgct tgcgccatcg
      601 gagccctgag ccgatgcgca cggcagtcca gcatgacaca ctttcggatt ctggtggagg
      661 cgtgattctc gtgtcgtgtc gggaaaagat cagggatgcg gatcgcgggg agcgcgaatc
      721 agcagaggcc ggcgggccgc tgccacgggt gctctccgtg cctgtgcccg ccgacgccac
      781 cggacgcacg gatgtgtggc tgacggcgca gttgccggag ttctcccgcg cgcgcttgca
      841 ggcgctgatc cgcgaggggc gggtgacctg ccgcggggtt gcggtcaagg cgaacgccag
      901 tccgcagccc ggcagtgtga tcgagatttg cctgcccgcg ccggttgcgg cggaacctgc
      961 gcccgaggcg attccgctcg atacggttta tgaggatcgc gattgtttgg tgatcaacaa
     1021 acccgccgga ctggtggtcc atccggcgcc cggccatgcg gcgggaacct tggtgaacgc
     1081 gctgttgcat cattgccgcg atctggttgg ggtggggggc gtgcaacggc ccggcattgt
     1141 gcaccgtttg gacaaggaca cttcgggtct catggtggtg gccaaacacg atgcggccat
     1201 ggccggtctg gtgcggttgt tccagaccgg cgagatccgc aaagagtatc tgactctggt
     1261 acacggcacg ccgccgcagg tttccggcac gcttcggacg cggatcgggc gtcatccggt
     1321 gcaccgcaaa aaaatggcgg tggtggaaac aggggggaaa gaggcggtga cgcactatac
     1381 ggtcgaaaag cggttgggcg ggctttcgct ggtgcgctgc cgcatcgaaa ccggccgtac
     1441 gcaccagatc cgcgtgcaca tgctgtcgct cggttgtccg gtggcggggg acgccctgta
     1501 cgggaagtcc gctgaggata agcggttgcc gcatgtgccc ccccgtcaac tgctgcacgc
     1561 cgcgcgtttg gcgttcgcgc atccccttac gggcgtggag ttggattttg ccgcgccgtt
     1621 gccagcggat ttcctgccgt ttcttgagga atccgaaggg ctacgtatct tcgtgtgaga
     1681 aacagccctg ccgcacgctt cggtgcggcc cctgcctgct gcgcaggcgg tcgaacccgt
     1741 gggttctcat ccctcccctc ctgattcgag ggcaagggga attggcggag agggagggat
     1801 tgccggtcgg ctgcgcctcc ctgccgcacg cttcggtgcg gcccctgcct gctgcgcagg
     1861 cggtcgaacc cgtgggttct catccctccc ctcctgattc gagggcaagg ggaattggcg
     1921 gagagggagg gattcgaacc cccggtacct tgcggcacgc atgatttcga gtcatgtgca
     1981 ttcaaccagg ctctgccacc tctccggacg aaaacggttg aactatactc aatctggacg
     2041 gagagggtca agagcgtgtt ttttgttgaa tccgcaaact ccggctgtgt tccggctaat
     2101 cttcttgcgg ccagcgcgtc tgccccgtat acttttgtga caaagtgaca ctgtgaaagg
     2161 gtgtgacacc atgtcgtccg gattttttga tagattggtg gcgcggatcg ataagctggg
     2221 tccggagagt ctgcaggcgc agatgatgcg tctggcgcgg gagcgcgggt ttttggagac
     2281 gatcttccag acgattcagg aagggatcat ggttctcgat tcggagggca agctgttgta
     2341 tgcgaaccga gcggcggaaa agctggcggg gttcgagttt tcgcgcacca agggccgttc
     2401 ggttctgagg ctgttgcgcg agtgggattg ggagcatctg ttcgatgtgc ccgaggggaa
     2461 cagtcagtgg aaccggatgg tgacgcgtga agtcgagatc acctatccgg agcaccggtt
     2521 cgtcagtgtc tatgccgtgc cgctggatga ggagggggtg tcggagaaag gcgtgctggt
     2581 gattttccgc gatgtgacgc gggaccgcct tcaggaagcc tcgacgctgg aaaacgaacg
     2641 ctcgaatgcc gtcaagctgt tggcggccgg ggtggcgcac gagatcggca acccgctgaa
     2701 tgcgttgaac atccatttgc agcttctggc ccgcgaattg agcagggtgc cggaggagac
     2761 acgcaaaccg ctggaggatc tggtggaggt ggcgcgcgcg gaggtcgggc gtctggacgc
     2821 gatcatcacg cagttcctgc gcgcgttgcg gccctccaag ccggtgcttc aaactgaatg
     2881 cccgaaggag gtgctgcagg agacgctgcg cgtgctgaag agcgagtttg aaaaccggcg
     2941 gatccaggtc gcggtggatg tgccggatca tcttccggca atgcggctcg accgcgctca
     3001 gattaagcag gtgttcttca atctcatcaa gaacgctctg gaggcgatgt cggatggcgg
     3061 tacgctcaaa gtggtgttca cggtggggga cgtgtttgtg gatatcgcgt tcatcgactc
     3121 gggcaagggg cttttgccgg acgagttcgg gcgggtgttc gatccttatt acaccaccaa
     3181 agccaagggg aacggattgg gattgatgat cgtgaagcgc atcattgagg agcacggcgg
     3241 cgagatcgaa ttgtccagca aaccggggga ggggacctgt ttcaagatcc gcctgccccg
     3301 ttcggaacgg cggatcaggt tgctcgggac tccgacgaag gaagagacgc atgaaaaaga
     3361 caggtaaacc ggtcgtgttg gtcgtggacg acgacaagaa cacgcgggac gggttgcagc
     3421 gcgcattgca gcaccactat gaggtgaagg cggtggagag cgcggacgcg gcgctggagc
     3481 tgctggccgg gggattgcag gcggacgtga tgctttcgga tctgcgcatg ccgggtatgg
     3541 acggactggg gctgttgcgg cgtgtgatgg cgcagtatcc ccgcacggtc tgcatcttgt
     3601 tgactgccta tggttctgtc gagacggccg ttgatgccat gaagcagggt gcctacgatt
     3661 ttttgaccaa accgatcaac cttgatcatc tcgatctgct ggtggcgcgg gccatcaagt
     3721 cccgcgacct cgaaaagaaa gtggagacgc tcgagaacca gttgaacgag aagtacggga
     3781 tggaaagcat catcggcgag tcgccggcca tgaggcaggt tttcgagatc atccgccaga
     3841 ccgcgcccac acaggcgtcg gtgctgatcc agggggcgag cggcaccggc aaggagctgg
     3901 tggcgcaggc gatccaccgg ttgagtcccc gcgccaaagg gcctttcgtg gccgtgcact
     3961 gtgcggcgct ttcgccaacc ctgctggaga gcgagttgtt cggtcacgaa cgcggggcct
     4021 tcacgggcgc gaccgtgcag cgtaagggcc ggttcgagat ggcggacggc ggcacgctgt
     4081 tcctcgacga ggtttcggag atcgaccctt cgatccaggt caagttgctg cgcgtgctgg
     4141 aggagcggac cttcgagcgg gtgggcggcg aggagaccat tgagaccgat atccgcgtga
     4201 ttgccgcgac caatcgggac ctccgtgcct atgtgaagga aggcaagttt cgggaagacc
     4261 ttttcttccg gctgaatgtg gtggatatcc tgttgccgtc gttgagtgaa cggacaggag
     4321 atatcccgct gctggcggac cggtttctga aagagtacac ggaaaggaac aacaggcaga
     4381 tcgacgggtt cacacccgat gcgatgggac tgctgaccgc ttacgcttgg ccgggcaatg
     4441 tgcgggaatt gcgtaacgcg atcgagaaga tggtggtgtt gtcgcggtcg gaccggctgt
     4501 ccgcgcggga tgtgcctgcc aacattcgcg aagcggtccg ggggtctgcg agtgccgcga
     4561 cccgcgcgcc ggcgttgctc tccggatcac tggccgacac cgagcggcga aaaatcatgg
     4621 cggtgctgga gaagaacgga gggaaccgca cacgggctgc ggaggagctg gggatcagcc
     4681 gtcggacgct gcaccgcaag ttacgggagt acaaggcgat gtcttccgat gtgtctgcgg
     4741 aggatgaggc gaaggaggat tggagccatg cgtagtccga cttcgttctc cctgccgaat
     4801 gtcgaggtgc tggaggtgat cgggcatggc ggcatggctt cggtatggaa agcgcggcag
     4861 gtctcgctgg atcggctcgt ggccgttaaa attctctcgc cggatttctc ctccgatccc
     4921 gaggacatcc ggcggttccg tcaggaggcg caggccgcgg cccggttgaa gcatccgggg
     4981 atcgtgcagg tgtatgacgc gaatttttcc gatggggtct actatttcat catggaattg
     5041 gtggaggggt atacgttggg caatctggtg cgtcgtaaag gccgtgtccc gatggaggat
     5101 gcgctcatcg tcgctgaaaa tgtggccgtg gtcctgaatt acgcttggga aaccgcgcag
     5161 atcatccact gcgacatcaa gcccgacaac atcatggcgg atgccgacgg cacggtgaag
     5221 gttacggatt tggggctctg ccgctcgctc acgttgcgca aggggagcac cacagacaag
     5281 gcggatgaga tcctcggcac accggcctac atgtcgcccg aacaggttta cggcagcgat
     5341 cggctggact gccgttcgga tatctacgca ttgggcgtga cgctgtatca tctggttacg
     5401 ggaagggtgt tgtttgacgg tcagtcggaa gacgagaccc tgcggagcca cacgggcgac
     5461 gcgcaggccc cggatgtccg cacgctcgcg ccggagacaa gtgaggcgtt cgccctgttg
     5521 ctcgagaaaa tgctggccaa gcaccctgat caccgtcacc gcgactggaa atctgtgttg
     5581 acggatctga gccgcctgcg ttcggggcat ctgccttgtc cggcgttgat tccgtccaag
     5641 ggcagttcga tgcttcggct gacgcaatga attctgggaa tgaggtgcgg gcttgttttc
     5701 atgagtccga ataaagaggg gagaagtcag gtatgcgaaa aaaaagattg tggatcgtag
     5761 tcatgatgtc ggcgctggcc ggttttgccg cagcgggtaa ggtcggtccg tgggagcgtg
     5821 cgccgctgta tcagacgccg caggtttggg atgcgccgga atacgccacg aacggcgtga
     5881 aggcggtttt ttatgagggc gaggtctatc agggaaagcc gacgcgcgtg tttgcctatt
     5941 acgccttgcc gaaggtggag ccgggcacaa aggtgccggg gatcgtgttg atccacggcg
     6001 gcggcggttc tgcctttatc cgttgggtaa aattgtggaa cagccgcggc tatgcggcga
     6061 tctcgatgga cacttgcggg gcggtgagcg gaaacgcgtt cggtgacgag cagagaggac
     6121 atcagcgtca tgcatgggcc ggccctgcgg gatggggcgg ttttgacaag tatgcggatc
     6181 cggtgcggga ccagtggacc taccatgcgg tggcggcggc agtgcgcggg cactcgctgt
     6241 tgcggtcgct gcccggcgtt gatcccgcgc gtatcggggt gaccggaatc tcgtgggggg
     6301 gataccttac ctgtatcgtg gcgggtgtgg acgaccggtt tgcgttcgcg gtgccggtct
     6361 acggttgcgg ttttctgggc gacaactcat gctggctgac gcggtttcag agcatgaaac
     6421 gcgagaatgc ggaaaaatgg ttggccctgt gggacccctc ggtctatctg ccgctggcga
     6481 aaatgccatt cctctgggtg acgggcagca acgatttcgc ttatccgatg gactcactgc
     6541 agaagagcta tcgggcactg aaggtcccgc gcacgctctg cgtccgtctg cgcatgccgc
     6601 acggccacgg tgccgcgggt gagaaccccg ccgagatcct cgcctttgcg gatcacatca
     6661 cgcggggcgc atccgccctg ccggagatca agcgcgtcca ccgcgaggac cgcaaggtca
     6721 ggatcacgtt caccggtaac cggcgtgtgg tcgtccgggc cgagttgaac catacggcgg
     6781 acacgggcat ctggaaggat cggaagtggg aagcggtgcc gtttgaactg aacgccggtc
     6841 tcgaagaggc ggcactcgaa atcccggcga cggcacgggt gtattacatc aaccttttca
     6901 ccgatgacgg cctcgtggtc agcagtgagc acgaggaact ctgaagcggg ttatgcggcg
     6961 atatgtcata cggcgcgtat tggagatgat cccgaccacg ttcggcattg tgttgctgac
     7021 gttcgtgctt tttcacgttg tgggcggatc gcccgcagag gtcgtgctgg gaaagaacgc
     7081 ttcggcagag gcgctggccg cgttcgacgc gcgctacggg tatgacaaac cgcttgtgtt
     7141 cgggcaatgg gtgcggctcc gtgccatgcg ggacattccg cgggcacacc tgccgcaggc
     7201 ggacgccggg gggcttcgac gtgttcccct ggcgtatgcg ctgcccgcag gttcataccg
     7261 tatccatgcg ccgcatgtcc tgatcgggtt gcgggagagc gcgaccggaa tgaccgaaga
     7321 cgcgatgatc gacggtcccg cgccgctgac gttcgcggtg cgcgaaggct ggcgggtcgt
     7381 gtcgctggat gtcggcgggg cgctgcccag cgtcatccgg ttgcgtcggc gcaccgcagg
     7441 cctttttgac tcgcagttca tgcgttacct cctttctctg gcgcgggggg atttgggcgt
     7501 ctcctccgaa cacaacttgc cggtggcgcg gattctccgc aacggcgtcg ggccgtcgct
     7561 ttcgctgacc atcccgatct tgatcggcgg cacgctggtg ggcgtgatgc tggggctggt
     7621 ctgcgccgca tggcgtggcg gcgtggcgga taaggcggtg ctgattctct ccacggttct
     7681 gatgagtgtc aattacgtgg tgtgggtttt ggcgggacag ttccttctgg cgttcaagct
     7741 gcggctcttt ccgatctggg gcttcgagtc agccgcgtat ctggtgttgc cggtgctgat
     7801 cgggatcgtc agcggattgg gacgggatgt ccggtttttc cgcgccgcga ttcttgacga
     7861 ggtatacaaa ccttacgtgc gcacggcgca ggccaagggt ctgggcgggc gccgcatcat
     7921 ggtgcgccac gtcttgcgca acagcctgat tccgatcgtc acctacgtca gcctctcgat
     7981 cccgttcctg ttcaccggca gcctgatgct ggaaagtttc ttcggcatcc ccggcctggg
     8041 cagtgtcagt ctgaatgcgt tgcactcgtc ggacatggct gtggtgcggg cggtggtggt
     8101 gttgggcgcg ctgatgtacc aaggtgtgaa tctggtcacc gaccttggct acgcctggct
     8161 cgacccgcgt gtgaggctgg ggtgacagcg gggatagagg caagaaccct gcagaaaact
     8221 ttcaacgttc aagcggaacc atgaaacacg tgaaacttca tgaaaatcag gggcttcctt
     8281 gacgttcatg gcgccttggc ggttcccaaa aaaccgagca atgccatctc taaaaaaatc
     8341 gcgtaatctg tgtccatctg cggatgagaa aacccgagcc atgccaaaaa caaactaccg
     8401 cactaccaca ctaatgaact accgcactaa tgaacttctc ttcaccctac tgcgggaagc
     8461 gcacacgata gaagcgcgcg ggacgggtgg cggcaccggg gtcggtgaag tcgaaggtgc
     8521 cggaggcggg gatggcgatg tcggcgacgg tgtcccagga aggggatgta aggctgtcgc
     8581 acgcttcgat gcggacgggg atgtcggcgt tgccggtgag ggagaaggcg aacgtaccgg
     8641 attcgaagcg cggcggtgac gcggggctga tcgcagggtt ccagcagacg acaggataac
     8701 tggcggcatc ccagccagcg gcactgggga ggtggtagag cgcggcgggg gtggtgtcga
     8761 aggcgtaggt gccgatgtct ggggcgtcac cggtgaaaag gacggtggtg agggcaccgc
     8821 agtaggagaa ggcgtcatca ccgactgacg tgacgcctgc cggaatgatg acagtagcaa
     8881 gcgattcaca accggtgaaa gcgtagtcgc cgatggatgt gagcgtatcg gggagggcga
     8941 tagcggtgag tgatgcacag taggagaagg cgtcatcgcc gatggctgtg ataccggttg
     9001 ggagggtgac ggattcaagg ccataacagc cggagaatgt ccctcgtccg atacgcctga
     9061 cgttatcggg aatgattacg tccaaaaggg atgagcagaa ggcaaaggca aaatcgccga
     9121 tggtcgtgac gctgtcagga agggtgacct cttcaagcga atggcagttg taaaaggcgt
     9181 aggttccgat gctggtgaca ccgtccggaa tgttggcgga ggtcaaggag gtacagtcgt
     9241 agaaggcata aggcccgatg gaggtgacgc tgtcggggat ggtaacggag gtgatggacg
     9301 cgcagttgcg gaaggcagag gggccgatag aggtgacagt gaggccagcg agtttctcag
     9361 ggatggacaa ggagccgggg aaggagggat cgctactggt gatgacagcg ccgccgttct
     9421 tttcggcgta cgtgagaagt tgcttggcgg ggcgacgggc gaaagtggag ccccatccag
     9481 atgtgccggg aaaataaaaa accgtggcgg gggtgtcgtc gaagccgtag ctggtagttg
     9541 gggcatttgg ggcatttccg cagaagagga gagtggtgag tgatgcgcag caggagaagg
     9601 cttcgtcgcc gatggttttg acgctgtcgg ggatggtgac ggaggtgagg gatgcgcaga
     9661 aggagaaggc ttcgttgccg atggttgtga cactatcagg gatagtgacg tatgcgaggg
     9721 atatgcagtc cttaaaggca ccttcgccga tgtttgtgac gccgtttccg atggtgacag
     9781 atgtgagcga ggtacaaccg tagaaggctg aggaatcgat ggttgtgacg ctgtcgggga
     9841 tggtgacgga agtaaggaca gtgcagttgt agaaagcata acctccgatg gttgtgacgg
     9901 tgtcggggat agtgaaatgg ccggagaacc ctacgggaca ttgaatcaac gttgaaaggg
     9961 ttttattgaa caagacgccg ttgagacttg cgtagttcag gttgtgctcc gatacgttga
    10021 tagctgtaag ggaagagcag taacggaagg caccgtaatc gatggttgtg acgccgtttc
    10081 cgatggtgac agatgtgagc gaggtacaac cgtagaaggc tgcgtaatcg atggttgtga
    10141 cactatcagg gatagtgacg tatgcgaggg atatgcattc ctcaaaggca ctttctccaa
    10201 tgttcatgac actgttgcca atgatgacgg tgttgaggga tatgcagtcc gagaaggcgc
    10261 tttttccgat atttgtgacg ctgtttccga tggtgacgga ggtgagggat gcgcagtaac
    10321 ggaaggcacc gtaatcgatg gttgtgacgc cgttgccgat aacggcattg gtaagggcgg
    10381 tgcagctgca gaaggcacca aaactgattg atgtgacgct gtctgggatg ttgacggagg
    10441 tgagagcggt gcagttgtag aaggcgtaat ttccgatgtt gatgacgctg ttgccgataa
    10501 cggcattggt gagggatgtg cagttgtaga aagctttgct cccgatgtta attacgctgt
    10561 ctgggatggt gacggagata agggatgagc agctatagaa agcgtagtca ccgatggcag
    10621 tgacaggaca accgccgagt tcgttggtga tggacaagtc gccggagtag ttactgtcaa
    10681 aatcggtgat ggtagccttg ccgttgctga cggtgtaggt gtaggggcct tcggtggcgg
    10741 cgggcacgac gagggggaga aacaacacga ggtgcgcgag cgtcaaggcg cggaggcgca
    10801 gcaagcgggg cgagcgtgtg aggcgtgatg cagtggggtt catggctggt ctttcgatgg
    10861 ttcgagaaca ggtgaagaac gcgtggacat ggtaagaccg gaggcgggag gatgcaagga
    10921 caaagagaaa aaacgctgaa cgcagaacgc agaacgcaga acgctgaagt gtgaaagcgg
    10981 aaccatgaaa cacatgaaac ttcatgaaaa ggcatggctc ggttttttta accgcgaatt
    11041 gcgcgaatca cgcgaatgat gcgaaagggc acggcgctgt ttttcgtgta ttaggggatg
    11101 acaaaatttg gagtgcggtg acaagagccg cgctgtgcgc gggttgcggc accgcttttg
    11161 cgcgctacct taccaccgct tctctgctca aaaacgccga agaaccttac agtctcacag
    11221 ctattta
//
LOCUS       DAOSRB010000087        11184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5731_length_11184_cov_7.220415, whole genome shotgun sequence.
ACCESSION   DAOSRB010000087 DAOSRB010000000
VERSION     DAOSRB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5731_length_11184_cov_7.220415"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..1297
                     /gene="nadA"
                     /locus_tag="PLZ74_06160"
     CDS             395..1297
                     /gene="nadA"
                     /locus_tag="PLZ74_06160"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOR97940.1"
                     /translation="MRIEQEIQTLKAERDAVILAHNYQPGEIQDIADFTGDSLELARE
                     ATHIRARVVVFCGVHFMAETAAILNPDKTVLLPAADAGCPMADMITGEQLRAMKARHP
                     DARVLCYVNSTAEVKAESDCCVTSANAVAVARSFPPQQPLLFVPDRHLGSYTAEQTGH
                     DLILWPGFCPTHARLTEAHIATARAAYPGAPVLVHPECPKPVRDAADAVLSTGGMCQW
                     VKERAEPVFLIGTETGILHRLRRENPDKTFIPLLEQAVCPYMKKTSVEHVLRSLRDLQ
                     TVITVPEPIAQRARRAIDAMLAIH"
     gene            1333..3552
                     /locus_tag="PLZ74_06165"
     CDS             1333..3552
                     /locus_tag="PLZ74_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97941.1"
                     /translation="MNTSNTTHTWHFFRSGGLDQALLRTGADLANLKTLDQKLWTALS
                     CPTTGIRFDAATLKLLDTDADGRIRASEILHAIDWLEKRLTSLDPLLVGGDAVNLAAI
                     HTGTAEGKALLSNAKRILKNLGKGDANAITLADVSDIAAIFAETRFNGDGVIPADAAD
                     APALRQTIEEIIACFGAETDRSGKPGINQTKTDAFFTAVAEHLDWVARPAADPAIRML
                     GDQTATAFDALQAVRAKIDDYFTRCRMAEYDPRAAQALSRTDADLAELADKELTDCHT
                     GLADFPLARIEAGRDLPLTAGVNPYWACALASFRDAVVTPLTDGAAETLSLSQWRELK
                     DRFAPFAAWQAACAGTEVASLGADRLEALRNGNDQAEITALIAKDVALEAEIAQITEV
                     EQLIRYHANLAALLNNYVNMGRLYDPEATALFQVGTLFMDARACNLCFEVDHVEAHAA
                     LAAASKCCLVYCSLTRPGAKTRTLCAAFTAGFAQTLWVGRNGIFYDVDGQDWDAVIIK
                     TVDNAISIKEAFWDPWRKIGTMISTQINKLLSARQDAALAAASKNVDAAATAKPAEPP
                     KKMEGAALASSVAALGIAVGLIGSAVGGLVSVLAGLPLWKTALGIMAVVLIVSGPSMI
                     LTWFKLRARDLAPILNACGWAINRRLRFSLKLGRLFTSEAVLPANSSRELMDPYADDS
                     RARNVLLVVLALVAVVAALWFTGLLDSVLPRRLQRQPPAACALRAPQAESPVSAVSP"
     gene            3549..4007
                     /locus_tag="PLZ74_06170"
     CDS             3549..4007
                     /locus_tag="PLZ74_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97942.1"
                     /translation="MTAWRTFPLAAMASALLFAGCATQRELIEKRIGQKHAFFETLPP
                     ETQQRLREGVLMAGDSEDAVWILYGTPDRVFQKVTGTTTNNIWSYVSYDAVPVETARP
                     ILYPVRTARGRVIWRRETFWSPDLFHHPYEYRRIEFRNGRVLSIEAEQQP"
     gene            4031..4501
                     /locus_tag="PLZ74_06175"
     CDS             4031..4501
                     /locus_tag="PLZ74_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97943.1"
                     /translation="MDKKDSGNFDFRHQAFARFAPATFIALAIPMLSLLPAAYFKRVI
                     PSAAFIPGWDKLIHALLYAALSRALFHALSPKAKERASCWTGVALAAGGYGLLMEFFQ
                     HGLTVSRGFDWFDALANLLGASIAAGLAGWRVRARHRRQGVEKEKDGPHRGPSF"
     gene            complement(4525..4935)
                     /gene="rplQ"
                     /locus_tag="PLZ74_06180"
     CDS             complement(4525..4935)
                     /gene="rplQ"
                     /locus_tag="PLZ74_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOR97944.1"
                     /translation="MRHKRLAKKFGRSMSHRKALMSSLVANLVLQDSIKTTLAKAKQA
                     RKDAEKMVTLARKGTLASRRLAAARLRSPSAVQRLFDSVVPAMDGRNGGYTRILKLGQ
                     RRSDGSEMCLLQWVVPPVKKAEAVEADAAAAKKS"
     gene            complement(4955..5950)
                     /locus_tag="PLZ74_06185"
     CDS             complement(4955..5950)
                     /locus_tag="PLZ74_06185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOR97945.1"
                     /translation="MPIRLSHFEMPRRVQKEENTATATYTRFVAEPFEVGYARTIGNS
                     IRRVLLSSIEGVAVTSVKIAGASHEFCALPGISEDVTDIILNLKRLLFKSYSRNPVWI
                     KLHKKGPCVVTAADFAEESAVEVLNPTQYIATLDTDGVLDLEAEIRIGRGFCPAEGNK
                     TPEQEIGRIPIDSVFSPVTRVNFLVENTRVGQRTDYEKLILDVWTDGRVSPEEALKTS
                     AAVLRHHLDVFVDYSEEVLEFEESEKKVDEERDRLRRILNMSVNEIELSVRAANCLNN
                     ANITTVGELAMKTEADMLKYRNFGKKSLNEIKDKLTELGLYLGYKFDPDLFEDNK"
     gene            complement(6016..6645)
                     /gene="rpsD"
                     /locus_tag="PLZ74_06190"
     CDS             complement(6016..6645)
                     /gene="rpsD"
                     /locus_tag="PLZ74_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOR97946.1"
                     /translation="MGRYTGPVCRICRREGQKLFLKGARCYMAKCAIEQGKPAPGQHG
                     TRRGRKISGYGEQLRQKQSLRRQFGMQELQFKRFFKEALRRKGVTGEILLQLLETRMD
                     NLVYRLGFASSRRAARQFVLHGHMAVNGKKVAVPSMILKVGDVIEVRDRARSRDLATR
                     SLEAATTQQTPAWLALDRKAFRAELLSIPTREQIAPIVDEQAIVELYSR"
     gene            complement(6647..7159)
                     /gene="rpsK"
                     /locus_tag="PLZ74_06195"
     CDS             complement(6647..7159)
                     /gene="rpsK"
                     /locus_tag="PLZ74_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOR97947.1"
                     /translation="MSEEEAKKEAKKEEEQAAAPEQQPEISEAQAVLSEGDDPTKTGT
                     ADVAKKKGKTKSIPAGIAFVRSTFNNTLVSITDARGGVIAWSSAGKAGFKGSRKSTAF
                     AATMVAQDAARQAIAKGMHEVEVRIQGAGAGRESAVRAIQSVGIHITLLKDCTPIPHN
                     GCRPRKRRRV"
     gene            complement(7237..7641)
                     /gene="rpsM"
                     /locus_tag="PLZ74_06200"
     CDS             complement(7237..7641)
                     /gene="rpsM"
                     /locus_tag="PLZ74_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOR97948.1"
                     /translation="MPRLMGVDIPGRKRVEYALRYIHGIGPKRARDVVEFCKIDPAKK
                     ADDLSPDEIRQIVAHIQDKYRVEGDLRREVSQNIRRLISIGTYRGLRHKRGLPVRGQR
                     TRTNARTRKGGKRTVGAVRDKMARAAIKAKSS"
     gene            complement(7657..7770)
                     /gene="rpmJ"
                     /locus_tag="PLZ74_06205"
     CDS             complement(7657..7770)
                     /gene="rpmJ"
                     /locus_tag="PLZ74_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOR97949.1"
                     /translation="MKVRSSVRRICERCRVVRRKGVVRIICTNPRHKQRQG"
     gene            complement(7832..8599)
                     /gene="map"
                     /locus_tag="PLZ74_06210"
     CDS             complement(7832..8599)
                     /gene="map"
                     /locus_tag="PLZ74_06210"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOR97950.1"
                     /translation="MAIPVKNAAELEAMRVSGRMTAEVLRQVAEAVRPGVTTGELDAL
                     TRRLIGEMGAKPSFLGYRGYPGAICISINDEVIHGIPGSRVVQPGDVVSLDVGVYYNG
                     FNGDSATTVMVGVRDPDTIRLVEATRRALAAGVAAVRPGARLSDVSHAVESVARASGC
                     SVVRDFVGHGVGRHLHEEPQIPNYGLPGRGPVLKPGMTLAIEPMVNLGRPEVAVLDDG
                     WTVVTRDGKPSAHFEHTVAVLEEGAEILTRSENFQTL"
     gene            complement(8599..9246)
                     /locus_tag="PLZ74_06215"
     CDS             complement(8599..9246)
                     /locus_tag="PLZ74_06215"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOR97951.1"
                     /translation="MKIVVLLGGPGSGKGTIATRLVAADRTFRHVSSGDLLREAVRLQ
                     TDAGREADGYMKRGELVPDELVAAMIREWMGRSGEGASTLLLDGFPRTVRQAEILDET
                     AAGCGAELSAAVLLEVDDATLFDRIAGRRGCPVCGAGYHVTNIPPKREGVCDVCGSAL
                     VTRKDDSFETVRNRLEVYRNQTLPLIDFYRARGVLRRVAGTGPIEGIVDRVRCAI"
     gene            9384..10055
                     /locus_tag="PLZ74_06220"
     CDS             9384..10055
                     /locus_tag="PLZ74_06220"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOR97952.1"
                     /translation="MSTPDLSCKRFLFPRMHPEGRAWVFAAAGIALLLTWIRLPLGLP
                     GVLLTYGVYLFFRDPERVPPADPRAVVSPADGIVSLITQLPFPIELTEDETPVWRVSV
                     FMSVFNVHVNRMPAAGTVVKTRYVPGAFFNASRDKASTDNERQLYLLRAHSGQLFAVV
                     QIAGLIARRIVRFVNEGQTLERAERFGLIRFGSRVDVYLPPGVTPTVRLGQVMVAGET
                     EIARL"
     gene            10079..10936
                     /locus_tag="PLZ74_06225"
     CDS             10079..10936
                     /locus_tag="PLZ74_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HOR97953.1"
                     /translation="MGKFRFRKRKHKVRARRGVPVRSMIPNLVTLMAAASGITSIRYS
                     CSGYEGHSRNAVIAIIIACVLDGLDGRVARMLRATSKLGAELDSLADFVSFGVAPALF
                     VYFWIMETVARDSAAYSFRGLFWALALFYAMCCAFRLARFNIMLDGGPTQPHWKPFFM
                     GLPAPGAAGLVLTPAIWHLHTGASFLQSPWIGGGMLLLCGILMASRFPTLSAKNLHVP
                     PRYLMPVLLTVMFLIGMLVSQFWLTLGIIGILYLLSIPVCGAVFRNLSRREHRKTAAA
                     PSPPPTAQD"
BASE COUNT         2017 a         3811 c         3134 g         2222 t
ORIGIN      
        1 ccgcgggcca ctgcgtgggc gcaacagtcg atttcgaacc cgatagcgat accgaacccg
       61 atagcgatac cgataccgat ttggagaacg tgaacgttgg ccataccccg ttcacgtacc
      121 cgtacccgta cacgataact cttccccctg tcacgaaccg attgcctttt ccggcttgtc
      181 tcagagtgta aatctggcta ccctctatcc gcaatgtgtg tgtaagacgc ttcaatctta
      241 cctgtttgcc gcacgaatgc gaggtcgcct ccaaaacctt aagagttgcg atagccagtc
      301 gcacacgtgc gtccttttct ttgttccgtc ataacgaaac ctgtggtacc gtagtgaaag
      361 gaataccgga caaatcattt gaggtggctt gatcatgcgg atcgaacagg agattcaaac
      421 gctgaaagcg gagcgggacg ccgtaattct cgcacacaac tatcagcccg gcgaaattca
      481 ggacatcgcc gacttcacgg gcgactcgct ggagttggcg cgcgaagcga cccatatccg
      541 cgcccgcgtg gttgttttct gcggtgtgca tttcatggcc gaaaccgcgg cgatcctcaa
      601 tccggacaag accgttctgc tgcctgcggc cgacgcgggt tgccctatgg ccgacatgat
      661 caccggtgag cagctccgcg cgatgaaggc ccggcacccg gacgcccggg tgctctgtta
      721 cgtcaacagc accgccgagg tcaaagccga aagcgactgc tgtgtcacat cggccaatgc
      781 cgtggcagtg gcccggagtt tcccgccgca acaaccgctg cttttcgtgc cggaccgcca
      841 tctcggctcg tacacggcgg agcagaccgg acacgacctt atcctgtggc cgggtttctg
      901 tccgacgcac gcgcggctga ccgaggccca tatcgcaacg gcgcgggccg cgtatcccgg
      961 cgcgccggtg ctggtccatc cggagtgccc taaaccggtc cgcgatgccg ctgatgctgt
     1021 gctgtcgacc ggcggcatgt gccaatgggt gaaggaacgg gccgaaccgg tctttctcat
     1081 cggcaccgaa accggcatcc tccaccgatt gcggcgggag aacccggaca aaacctttat
     1141 tccgctgctc gaacaggccg tctgtccgta catgaaaaaa acctcggtag agcatgtgct
     1201 caggagtctg cgggacctgc agacggtcat caccgttccg gaacctatcg cacaacgtgc
     1261 cagacgcgcc atcgacgcaa tgctggccat tcactgaacc ccaatcaatg tggctcgcaa
     1321 ggacaagata gcatgaacac ctccaacacg acgcatacgt ggcatttctt cagaagcggt
     1381 ggtcttgacc aggcgcttct ccgtaccggc gcggatctcg ctaacttgaa aaccctggat
     1441 cagaaactct ggaccgccct gagttgcccg accaccggca tccgttttga cgccgccacc
     1501 ctgaagttgt tggacaccga tgcggacggc cggattcgcg cgtcggaaat cctgcacgcc
     1561 atcgactggc tcgaaaaacg cctcacctcc ctcgatccgt tgctcgttgg cggcgacgcc
     1621 gtcaacctcg ccgcgatcca taccggaacc gcggaaggaa aggccctgct atccaacgcg
     1681 aaacgtattc tcaagaatct cgggaaaggc gatgcgaacg ccatcaccct ggctgatgtg
     1741 agcgacattg ccgcgatctt cgccgagacc cggttcaatg gcgacggtgt gattcccgca
     1801 gatgccgcag acgcgccagc gttgcgccag acgatcgaag agatcatcgc atgtttcggc
     1861 gcggagaccg accgcagcgg caagcccggt atcaatcaga cgaaaaccga cgcattcttc
     1921 accgctgttg cggagcacct cgactgggtg gcccgccccg ccgctgaccc ggcgatccgg
     1981 atgctgggcg accagaccgc cacggcgttt gacgcgctcc aagcggtacg cgccaaaatc
     2041 gatgactact tcacccggtg ccgcatggcc gaatatgatc cgcgcgccgc gcaggccctc
     2101 agccgcacgg acgccgattt ggccgagctg gccgacaagg aactcacgga ttgccacacc
     2161 gggttggcgg attttcccct ggcccggatc gaagccggcc gggatcttcc cctcaccgca
     2221 ggggtcaatc cgtattgggc ctgcgcgctg gcctcgttcc gtgatgcggt cgtgaccccg
     2281 ctcacggatg gtgcggcaga aacgctttct ctctcgcaat ggcgcgagtt gaaagaccgg
     2341 ttcgccccct tcgcggcctg gcaggccgcc tgtgccggca ccgaagtcgc ttcccttggc
     2401 gcggaccggc tggaagccct gcgcaacgga aacgatcagg ctgaaatcac ggcgctgatc
     2461 gccaaagatg tggcgctgga agccgaaatc gcgcaaatca ccgaggtgga gcaactgatc
     2521 cgctaccatg cgaatctagc cgcgttgctg aacaactacg tcaacatggg acgcctctac
     2581 gacccggagg ctaccgcgtt gtttcaggtc ggcacgctct tcatggatgc ccgcgcctgc
     2641 aacctctgct tcgaggtcga tcatgtggaa gcccacgccg cactggctgc ggccagcaaa
     2701 tgctgccttg tctactgttc cctcacgcgg cccggcgcca aaacccgtac cctctgcgcg
     2761 gcctttacag cgggcttcgc gcagacgctc tgggtcggac gaaacggcat cttctacgat
     2821 gtcgatgggc aagactggga tgctgtaatc atcaaaaccg tggacaacgc catttcgatc
     2881 aaagaagcgt tttgggatcc gtggcgcaag atcgggacga tgatcagcac acagatcaac
     2941 aagctgctct cggcgcggca ggacgcggcg ctcgccgccg catccaaaaa tgtcgacgcc
     3001 gccgcaaccg cgaaaccagc cgaaccgccc aaaaagatgg agggcgcggc cctggcctcg
     3061 tcggtcgcgg ccctcggcat cgcggtcggc ctgatcggtt ccgctgtcgg cgggctcgtc
     3121 agtgtgctgg cgggattgcc gctctggaag acggcgctcg gcatcatggc cgtagtgctg
     3181 atcgtctcgg gaccttccat gatcctcact tggttcaaac tgcgcgcgcg cgatctcgcg
     3241 ccgatcctca atgcctgcgg gtgggccatc aaccgccgcc tgcgcttctc gctcaagttg
     3301 ggccgccttt tcacgagtga ggccgtgctg ccggcaaatt cgtcacgcga actgatggat
     3361 ccgtacgcgg acgattcgcg tgcgcggaac gtgctgctgg tggtcttggc gcttgtcgcg
     3421 gtcgtggcgg ccctctggtt cacagggctg ctcgactccg ttttgccgcg ccgcctgcaa
     3481 cgccagccgc ccgccgcgtg cgcgctccgc gcgccgcagg cggaatcccc cgtctctgcg
     3541 gtgagcccat gactgcgtgg cgaaccttcc cgttggctgc gatggcaagc gccctcctgt
     3601 ttgcgggatg cgccacccag cgcgagctga tcgaaaaacg catcggccag aagcatgcgt
     3661 ttttcgagac gctgccgcct gaaacccagc agcgcctgcg ggaaggcgtg ctcatggcgg
     3721 gggattccga ggatgcggtc tggatcctct acggcacccc cgaccgcgtg ttccagaaag
     3781 tgaccggcac aaccaccaac aacatctggt cgtatgtctc ctacgacgcc gtgcccgtgg
     3841 agaccgcgcg ccctatcctg tatccggtcc gaaccgcgcg cggacgagtc atctggcggc
     3901 gcgaaacctt ctggtccccc gacctcttcc accaccctta cgaatatcgg cgcatcgagt
     3961 tccggaacgg ccgcgttctg tccatcgaag ccgaacaaca gccgtaacac gatcccgatc
     4021 gattatggtc atggacaaaa aggattctgg aaacttcgat tttcgccatc aggcctttgc
     4081 ccgttttgcc ccggcgactt tcatcgcgct ggcgatcccg atgctctcgt tgcttcccgc
     4141 cgcatatttc aagcgcgtca taccttccgc ggcgtttatt ccgggatggg acaagctgat
     4201 ccacgccctg ctctatgcgg cgctgtcccg cgcgcttttt cacgccctct cgccaaaggc
     4261 caaagaacgg gcctcctgct ggaccggcgt ggcactggcc gcaggcgggt acgggctgct
     4321 catggagttt ttccagcacg ggctgaccgt gagccgtgga ttcgactggt tcgacgcgct
     4381 ggccaatctg ttgggtgcgt cgatagcggc ggggctggcg ggctggcggg tacgcgcgcg
     4441 ccatcggcgg cagggggttg aaaaggaaaa agacggcccc catcggggac cgtcctttta
     4501 gaccgcgtca ggcaggcagt cggctcagct tttcttcgcc gccgcagcgt ccgcttcaac
     4561 agcctcggcc ttcttcaccg gaggcacgac ccactgcaac aggcacatct ccgagccgtc
     4621 actgcggcgt tggccgagtt taagaatccg cgtgtagcca ccgttccgac cgtccatcgc
     4681 cggcacgacg ctgtcaaaca gcctctgcac ggcggacggg ctccgcagac gcgccgccgc
     4741 aagacgccgt gaggccaagg tccctttccg ggccagcgtg accatcttct cggcatcctt
     4801 acgggcctgt ttcgctttgg ccaacgtcgt cttgatgctg tcctgcagca ccagattcgc
     4861 cacaaggctg ctcatgagcg ccttacgatg tgacatggac cgcccgaact ttttcgctaa
     4921 tcttttatga cgcatctctc agaacctcaa acgtttactt gttatcttcg aacaggtccg
     4981 ggtcgaactt gtaccccaga tagagcccca actcggtgag tttatccttg atctcgttca
     5041 gcgatttctt gccgaagttc cggtacttca acatgtcggc ctcggtcttc atcgccaact
     5101 cgcccaccgt ggtgatgttc gcgttgttca ggcagttcgc ggcgcggaca ctcaactcga
     5161 tctcgttcac gctcatgtta aggatacggc gcagacggtc acgctcctca tcgaccttct
     5221 tctccgactc ctcgaactcc agcacttcct cgctatagtc cacaaacacg tcgaggtggt
     5281 ggcgcaacac ggccgccgag gtcttgaggg cttcctccgg cgagacacgc ccgtcggtcc
     5341 agacatcgag gatcagcttc tcataatccg tgcgctgccc cacccgggtg ttttccacga
     5401 ggaaattcac acgggtgacc ggagagaaaa cgctatcgat cggaatgcgc ccgatctcct
     5461 gttccggcgt cttgttgccc tcggccggac agaacccgcg tcctatacgg atttccgcct
     5521 ccaggtccag caccccgtct gtatccagcg tggcgatgta ctgagtgggg ttgagcacct
     5581 ccaccgcact ctcctcggca aaatccgccg ccgtcacgac gcacggcccc ttcttgtgca
     5641 acttgatcca gaccgggtta cggctatagc tcttgaacaa cagccgcttg aggttcagga
     5701 tgatgtccgt gacatcctcg ctgatgccgg gcagggcgca gaactcgtga ctcgcgcccg
     5761 cgatcttcac ggaggtgacc gccacgccct cgatagagga caacagcaca cgtcggatcg
     5821 agttgccgat ggtgcgggcg taacccacct caaacggctc cgcgacaaaa cgcgtatagg
     5881 tcgcggtcgc ggtgttctct tccttctgca cgcgccgggg catctcaaaa tggcttaaac
     5941 gaatcggcat gttcccattc ctttcagacc gcccgccccc cctttgggag acggcggccg
     6001 tcagtacatc tgcgcttaac gcgagtacaa ttcgacaatc gcctgctcat ccacgatcgg
     6061 cgcgatctgc tcgcgcgtcg ggatactcaa caactcagcg cggaaggcct tcctgtccag
     6121 cgcgagccag gccggcgtct gctgggtggt ggcggcctca aggctgcgtg tggcgaggtc
     6181 tcggctacgt gcgcggtccc ggacttcaat cacatccccg accttgagga tcatggaggg
     6241 gaccgcgacc ttcttgccgt tcaccgccat gtggccgtgg agcacgaact gacgggcagc
     6301 gcggcgcgaa gacgcgaaac cgagccggta aaccagattg tccatgcggg tttccaacaa
     6361 ctgcagcaag atctcgccgg tgaccccttt ccggcgcagg gcctctttga agaaccgctt
     6421 gaactgcagt tcctgcatgc cgaactggcg tcgcaggctc tgcttctggc ggagttgctc
     6481 cccgtatccg gaaattttac ggccgcggcg cgtgccgtgc tgaccgggcg ccggcttgcc
     6541 ctgctcgatc gcgcacttcg ccatgtagca ccgtgcgccc ttcaaaaaca gcttttgccc
     6601 ctcgcgccgg caaatgcggc aaacggggcc agtatatctt cccatgttag actctcctcc
     6661 gtttacgcgg acggcacccg ttatggggga tcggcgtgca atccttgagc agggtgatgt
     6721 gaatgccgac ggactggatc gcgcgcacgg ccgattcgcg gcccgcgcct gcgccctgaa
     6781 tcctgacttc cacttcgtgc atccctttgg cgatcgcctg acgggcggca tcctgcgcta
     6841 ccatggtggc cgcgaacgcg gtgctcttgc gcgatccctt gaatccggcc ttcccggccg
     6901 aactccaggc gatcaccccg ccgcgggcat ccgtaatcga taccagcgtg ttgttgaacg
     6961 tcgaccggac aaaggcgatg cccgcgggaa tcgactttgt cttgcctttt ttcttagcca
     7021 catccgcggt accggttttg gtggggtcat ccccctcgga taacacggcc tgcgcctcac
     7081 tgatctccgg ctgctgctcg ggagcagcgg cctgctcttc ttctttcttc gcttctttct
     7141 tcgcttcttc ttcgctcatg cgtctcagcc tttaaatggt gtggcgggca ccttccccct
     7201 gcgctaacag cagagagccg tgccgacgcc gggttgttag gagctcttgg ccttgatggc
     7261 cgcccgtgcc atcttgtcac gcaccgcacc caccgtccgt tttccaccct tccgggtccg
     7321 cgcattggtg cgggtccgtt gcccgcgtac cggcagcccg cgcttatggc gcaatccgcg
     7381 gtacgtccca atgctgatca gacggcggat gttctgcgaa acctcacgcc ggagatcgcc
     7441 ttccacccgg tatttgtcct gaatgtgcgc cacgatctgc cggatctcgt ccggcgagag
     7501 atcgtccgct tttttggccg ggtcaatttt acaaaactcg accacgtcac gggcccgttt
     7561 gggaccgatt ccatgaatat accggagcgc gtattccaca cgcttcctgc ccgggatatc
     7621 cacacccatc aatctcggca tggttgtcct cttccgttaa ccttggcgct gcttatggcg
     7681 gggattcgta caaatgatgc gaacgacgcc tttgcgacgt accacccggc aacgctcaca
     7741 aatgcgccgc actgaactgc gaaccttcat ggtcttttcc cttgttcaaa aaaaggtttt
     7801 agattgaacc aaaagtccgt cgccctgtct atcacaaagt ttggaaattc tctgaccgcg
     7861 tcagaatctc cgccccttct tcgagcacgg ccacggtgtg ctcgaagtgc gcggacggtt
     7921 tcccgtcccg cgtcaccacg gtccacccgt catccaaaac ggccacttcc ggcctgccga
     7981 gattgaccat cggctcaatc gccaacgtca tccccggctt caggacggga ccgcgtccgg
     8041 gcaacccgta gttcgggatc tgcggttcct cgtggagatg acgccccacg ccgtgaccga
     8101 cgaaatcccg gaccaccgaa caccccgatg cccgcgcgac gctttccacc gcatgcgaaa
     8161 catcggagag ccgggcaccc ggcctgacag cggccactcc cgccgccaat gcgcggcggg
     8221 tcgcctccac cagacggatc gtatcgggat cccggacccc caccatcacg gtggtggccg
     8281 aatccccgtt gaacccgttg taatacactc ctacgtcaag gctcaccacg tcacccggtt
     8341 gcacaacccg gcttcccgga atgccgtgga tcacttcatc gttgatcgaa atacaaatcg
     8401 cgcccggata cccgcggtac ccgagaaacg agggtttggc ccccatctcc ccgatgagcc
     8461 gccgggtcag ggcatccaac tcacccgtcg tcacgcccgg ccgcaccgcc tccgccacct
     8521 gccgcaggac ctccgcggtc atcctgccgc tcacgcgcat cgcctccaac tccgcagcgt
     8581 ttttcaccgg tatcgccatc atatcgcgca cctgacccga tccacaatcc cctcaatcgg
     8641 ccccgtgccg gccacccgcc gcaacacgcc tcgcgcccga taaaaatcaa tcaggggaag
     8701 ggtctggttc cgatacacct ccagccggtt ccgcaccgtc tcgaaactgt cgtctttccg
     8761 ggtcaccagc gcgctcccgc acacatcgca cacgccttcc cgcttgggcg ggatattcgt
     8821 cacgtgatac cccgccccgc atactggaca accgcgccgt ccggcaatcc ggtcgaacag
     8881 ggtcgcgtcg tccacctcaa gcaacacggc cgcggaaagc tcggccccgc aaccagccgc
     8941 cgtctcgtcc aaaatctcgg cctgccgcac cgtgcggggg aatccgtcca gcagcagagt
     9001 gctcgcaccc tcgccggacc gccccatcca ttcccggatc atcgcggcga ccagttcgtc
     9061 cggcaccaac tcgccccgct tcatataccc gtccgcctca cgccccgcat ccgtttgaag
     9121 ccgcaccgcc tcacgcaaaa gatctccgct cgaaacatga cggaacgtgc ggtccgcagc
     9181 caccagccgc gtggcgatgg tgcctttccc cgaacccggt ccacccagaa gaacgactat
     9241 tttcatgtat gggcgtgtaa ctttatcagg tccgcgcacg cgacgcaacg cgaaatcttt
     9301 cgcggtcgcc gccgattttc gttccgttct tgcattgccc cccctctcct gacatactgg
     9361 ttttttcatt ggggttttct cttgtgagta caccagattt gtcctgtaaa cgctttcttt
     9421 tcccccggat gcacccggag ggccgcgctt gggtgttcgc ggccgcaggg atcgccctgc
     9481 tgctcacatg gatccgcctt ccgctgggtc tgccgggagt gctgctgacc tacggggttt
     9541 acctcttttt ccgcgacccg gagcgtgtgc cgcccgctga tccccgcgcc gtggtcagcc
     9601 cggcggacgg gatcgtcagc ctcatcacgc agctcccttt ccccatcgag ctgacggagg
     9661 atgaaacacc ggtctggcgc gtcagcgtct tcatgagcgt gttcaacgtc catgtcaacc
     9721 ggatgcccgc ggccggaacc gtggtgaaga cccgttacgt gccgggtgcc tttttcaacg
     9781 cgtcacgcga caaggccagc acagacaacg aacgccagct ttatctcctg cgcgcgcatt
     9841 cgggccaact gtttgccgtg gtgcagatcg cggggctcat cgcccgccgc atcgtccggt
     9901 ttgtcaacga aggccagaca ttggaacggg ccgagcgttt cggcctgatc cgcttcggca
     9961 gccgggtgga tgtgtatctc ccgcccggcg tgacgcccac cgtccgtctc gggcaagtga
    10021 tggtcgcggg cgaaaccgaa atcgcccgac tctaacacac gtttttccag ataccattat
    10081 gggcaaattc cgtttccgca aacgtaagca caaggtccgc gcgcgccgtg gcgtgccggt
    10141 ccggtcaatg atcccgaacc tcgtcacgct gatggcggcg gcgagcggca tcacctcgat
    10201 ccgttacagt tgcagcgggt atgaggggca cagccgcaac gccgtgatcg cgatcatcat
    10261 cgcctgcgtg ctggatggct tggacggccg ggtggcgcgc atgctgcgcg ccacttccaa
    10321 actcggcgcg gaactggatt ccctcgccga tttcgtgagc ttcggcgtgg caccggccct
    10381 tttcgtctac ttctggatca tggagaccgt ggcgcgcgac agtgccgcct attcgttccg
    10441 cggcctgttc tgggcgctgg cgcttttcta cgccatgtgc tgcgccttcc gcctcgcgcg
    10501 gttcaacatc atgctggacg gcggtccgac ccagccgcat tggaaaccgt ttttcatggg
    10561 cctgcctgcg cccggcgccg cgggactcgt cctgacgccc gcgatctggc atctccacac
    10621 cggagcgtcc ttcctgcaat ccccgtggat cgggggaggc atgctgctac tctgcggcat
    10681 cctcatggcc agccgttttc cgaccctctc cgccaaaaac ctgcacgtgc cgccccgcta
    10741 tctgatgccg gtactgctga ccgtcatgtt cctgatcggc atgctcgtct cccagttctg
    10801 gctgaccctg gggatcatcg gcatcctcta cctgctctcc atccctgtct gcggtgcggt
    10861 cttccggaac ttgtcgcgcc gggaacacag gaaaaccgct gccgcgccgt ctcccccgcc
    10921 cactgcccaa gattgacgaa tgttggacat cgaacgccga actccgaaag tgcggcaggg
    10981 acgcctcccc gaggcgtccg catggcccgg cggcggttta cggggaaacc gccctacctt
    11041 ttgtcacgtg gtgaagcaat cgatcccgat agcgatcccg atcccgaccc cgatgaaacc
    11101 ctgaaacctt gaaacctgaa accttgaaac ccgatagcga tcccgatccc gaccccgatt
    11161 tggatgaaac cctgaaacca gaaa
//
LOCUS       DAOSRB010000088        11128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5764_length_11128_cov_6.593064, whole genome shotgun sequence.
ACCESSION   DAOSRB010000088 DAOSRB010000000
VERSION     DAOSRB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5764_length_11128_cov_6.593064"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLZ74_06230"
     CDS             complement(<1..649)
                     /locus_tag="PLZ74_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR97954.1"
                     /translation="MLEATVLFKTYTLPHKRIEVLKGASLHIRKGERVAIVGRSGAGK
                     STLLHVLGGLDRPESGEVRIGGVSLYEVSPRRRTAARAAQIGFVFQSYHLLPEMDVTE
                     NVMLPAMTGVTRLTRREMRLRAQTLLGQVGLADRATHMPLELSGGEQQRVALARALIN
                     EPALILADEPTGNLDRLTGGQIMELLFSLSCTRDLALVMVTHSPETAALCDRVLEL"
     gene            complement(662..1921)
                     /locus_tag="PLZ74_06235"
     CDS             complement(662..1921)
                     /locus_tag="PLZ74_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR97955.1"
                     /translation="MNSRAYALTAPMPFSLFLALKYLKPKRSFTSVVTLISVIGVVLG
                     VAIIIIVRAVMTGFGDMWKEKILDFKPHITILSRNGGVIEGEGEICRRLEQVPGVTAV
                     SPGLETRVLVEHRRRVVAPLLIGTDPARAQHVMKLDRLAAGAFELEGDAVVLGIDLAA
                     ELGIRVGEDLLVYSPMNLVSRDELFFPERLTVTGIYNSGQRDFDSGFMITSLAVVRDL
                     MGMRSGVYSIHLKVADPQNTVGFSRLVSEIRALLPIYSVRTWQEIDSQLFNALAVEKN
                     MMVILLMFITVVAIFCVTNTLIVLTVQKTDEIGLLKALGFSSRQVMMAFVLHGWIQCL
                     IGTALGIGAALLILENLQGLVSLLGRFGLEVFPKDVYGLAQIPWRVIPREVTDVALSV
                     ILFCTVASFLPAWRAARMDPVTALRKE"
     gene            complement(1969..3678)
                     /locus_tag="PLZ74_06240"
     CDS             complement(1969..3678)
                     /locus_tag="PLZ74_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOR97956.1"
                     /translation="MSSYLDMNEVNSRLGEIIEMYTEDSYLRRLKKLFSGLKAPRHSR
                     EYKMAMIELQRLSAPLTAILLPVFAVVMLSVMDGGATGPDRLIETQVLEAEEIKDLDK
                     VQEELERPEEPMEEVDIDIPVANPNVPVPTDMPTDQPVSPQPQAFDAVMMVKSPVILK
                     NIYGTTRSTGTRGTQLARFGGDAFTEEAVMRALRWLKKNQQADGSWNRNKVAMTGLAI
                     LAFLSHGEKPGDSAEFGETVQKGLEYLMRVQNKKTGRLPGNYDHPIAAYALCEAYGMT
                     INPNVKTAAEMAMVPIIKGQHPTGGWTYHMDPNPDKETGKYRDDTSYMGWCAQAIKAA
                     QLARLQVDGLEKASKLAIRGFKANAGPNGGFGYTGPGAGGLTSVGTLCMQLLGASNEP
                     EVRKSLDLMDSWVPTFSSYTILADELAKIEKSQLKQNQTPLSVAKARVIKTLPPEEQK
                     FFNASPQYYFYYATQCKFHAGGKRWDSWNKQMKPNYVKAQQVKKDAIQDHKGALRDIG
                     WWENTDAHSDRPVMDTCLAALQLMVYYRYLPTTSKEAVQVEAEISASTTDTGDIQVDT
                     GNL"
     gene            complement(3700..4107)
                     /locus_tag="PLZ74_06245"
     CDS             complement(3700..4107)
                     /locus_tag="PLZ74_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOR97957.1"
                     /translation="MAYKRKKNKGESPKLDMTPMIDVVFQLLIFFVVTLKQEDILSKL
                     SAARPAANPDEKVKEQQQDLINVIVAPQGFVFNGRPMRLPELDRSIERLSGYSKTAMV
                     IIKCYADSTHAMLIQVLDVCNKHGMTNLSIFSM"
     gene            complement(4125..4553)
                     /locus_tag="PLZ74_06250"
     CDS             complement(4125..4553)
                     /locus_tag="PLZ74_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOR97958.1"
                     /translation="MAKEYEQCEIDMTPMIDVVFQLIIFFVVTMKMNQEFNQDIVLED
                     GKHGVTITAENMPPTTLEIELDRRGRVSIHNATLTEKNLRDILRARVNKHGNEFPVLI
                     RADRRTLHEKVRKLMDVCTSCGVWKLSFVAVQEHKARDDR"
     gene            complement(4707..5507)
                     /locus_tag="PLZ74_06255"
     CDS             complement(4707..5507)
                     /locus_tag="PLZ74_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOR97959.1"
                     /translation="MMKRLVMSAVLVAGLMMGASGVSAQDAAPAAAAAAAPAAVESAQ
                     PTESAKKSSKDSTGFLAVVLGSGWLGAVLWLALFGCGAAAIYFIVDCSILIRPQKIMP
                     QALIAKVKGAMAEGDVMKALHACESEPGPMANILSAGFSHVEEGFEVIQESIGAAADL
                     ETERIMQRLTWISVCSNLSPMLGLLGTVQGMIMCFGTLATGAPDVAALALAIAQALWT
                     TAGGLAIAIPAITCYYSIRNNANRLILRMQAMTMELIKDLRNVEVVSD"
     gene            complement(5585..6391)
                     /locus_tag="PLZ74_06260"
     CDS             complement(5585..6391)
                     /locus_tag="PLZ74_06260"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR97960.1"
                     /translation="MKMSTQTGRVAVALAMLTAGTAAAQIAGVITTSEGRKIEGVIKW
                     KVREKAYAITAKGGNVELEMSPANISDVQIPRPKELDAAIANMNQGNAAAAIPVLEKL
                     ANDYLMLQWDKPATRLLAEAYVASGNADKARQVCEKVIAANPEAAYLGEMAPAYWKAL
                     LKSGRVTKVEDLMAKAIKSGDRAASASALIMRGDVILAGGETNENAKKALRDGFLRVI
                     TLYKSEKDVQPEALYKAAKCFDNLGQSSRADQMRTALKSEYGSSEWAHKR"
     gene            complement(6418..9423)
                     /locus_tag="PLZ74_06265"
     CDS             complement(6418..9423)
                     /locus_tag="PLZ74_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR97961.1"
                     /translation="MISGHGLALLWCAIVLSAFLSRAQGDVDNQELREELKYIDALQQ
                     LQMPDLAAEVIAEAKKRFPEAAAQFKIREFQGLLWQGKFDEVRKVVEALPDKNGEEYW
                     ALNLAMADAYYAFGKYMDADKLYLAFFKTFSKPPAALTSFYRDSAYKHAQMLIRLGKQ
                     TEALAAFKRVFNVKLEEEVERQVLADMAELMLKIAPNLSKKGEKDAMLKEAEAAVDKL
                     LWKQDVWFGKAIVMKAHIFLLRGDVKGAQDLVENYMPQLRTIHDSLVEQDADGSLGML
                     RMSPMPQCRYLLAVLLMNEAVAEAQKEGGSDDRIKDLLLGERDPKTRQRKGNGAFNHF
                     INVFIRYPESQWAAEAGERSEALRKLIKERYNTDIRTPVTVEQMAKVRQMQFAGARLL
                     FSQSQFKEAIDKYLLVLNQFPETPESVPALGDLAISYIETAGKDPDALLMADTVTGHL
                     SERFCAKEEMMKEAGDQVRRIGDRFGEMAMEDKKRETYALFFRDYPTHYAAGQLVMSF
                     GEREFHAKNYTGALTYYQLITDQYQHSTYYFDALNRIAQVYREEGDRTNEIQALEVYV
                     SRLAERDRPGNPLVVGKFRLADAQREYGNALMRAAVTNEQAEATQKEAVLWLTRAAVG
                     FDGVAKLLREKAGEYQNNAEEKQRNAQILEMSVFTKAVCLAQVNFPPERLTQFRKLAI
                     AAFEEYVKQYPEGKLAPRAQLQIGTLHTILEDASNAQAAFEKLSKQYPQSEEARNSVP
                     LLAASLIEMGLRGEGVAKYREMFGAGGKYTEGQFMAAAQALEQAREYDMALQAYDRVL
                     ATAKDLPVVANAKLGRARVLVGQKKYQEAHKALDAFIQDKALSNLEIVVDANMLLVEV
                     ASEEGRHEKDDNERTRLFNTAIDALKMVKKYRKEPEEMVELDLAAGQILLRKMEAEKK
                     LGLKEQVAETRGKAIVAFQVMIMNINPGNAKLASTLEKAYFYCVPLLLEHKKFKDAEE
                     DCETYLRIFPNGRYATDIRNWLNQAKIGQ"
     gene            9655..10788
                     /locus_tag="PLZ74_06270"
     CDS             9655..10788
                     /locus_tag="PLZ74_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97962.1"
                     /translation="MGKLLRVLVVVILLLSIVSLVFANLLFGKRELLIQRNSALEEHV
                     IRLSKTLEASDPQDVAAPDVQKDNSDVTDREIANPDRVSVLENYPIKLEQQNLPTLDF
                     GSTEKRIQLRSYYALTSEGKIDPDPVDQRPRTKGPGTMQELLDLAFERAKVQQATLNK
                     TRAELTKMRERVTGVVEEVNKLKAEGRTVKRDLKGAREQVAALETEKGELNGRITKLT
                     AEKRELAAEAADAKDQAERLNEEKLTLTEDLTKTREALEEMKKRFAGAGSRPVQESSG
                     VMVTALSAGDKGKILEANDELKFAILEFTDDAMTEMLGPERQNAMPQFEMNVRRIGRQ
                     SASGEFVTRIKLRQAVRGKNLVVADILSDWQQAPVEKGDVVFF"
     gene            10772..10954
                     /locus_tag="PLZ74_06275"
     CDS             10772..10954
                     /locus_tag="PLZ74_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97963.1"
                     /translation="MSFSSKAVRASLGLALLPTLALLLSGCMSDQPTETDMPWAAPAA
                     WEGTMPLPGGFMNRYE"
BASE COUNT         2135 a         3527 c         3168 g         2298 t
ORIGIN      
        1 gcaactccag cacacggtcg cagagcgccg cggtctcggg tgaatgggtc accatcacca
       61 atgccagatc gcgggtacag gagaggctga acagcagttc catgatctgc ccgccggtca
      121 ggcggtccag attgccggtc ggctcatcag ccaaaatcaa cgcgggctcg ttgatcaggg
      181 cgcgggccag agccacgcgc tgctgctcgc cgcccgagag ttccagcggc atgtgggtgg
      241 cgcggtcggc gaggccgacc tgccccaaca gcgtctgggc acgtaaccgc atctcccggc
      301 gtgtcaggcg ggtgacaccc gtcatggcag ggagcataac attttcggtg acatccattt
      361 ccggcagcag gtggtaggac tggaacacaa agccgatttg cgcggcacgc gcagccgtgc
      421 gccgccgcgg cgagacctca tagagcgaca cccccccaat gcgcacctcg ccggactcgg
      481 gccggtccag accgccgagc acatgcagca gtgtgctttt tcccgccccg ctgcggccca
      541 cgatcgccac gcgctcgcct ttgcgaatat gcagcgaggc gcctttcagc acctcgatcc
      601 gcttatgggg aagcgtgtag gtcttgaaca acactgtggc ttctaacatc acggcctttc
      661 ttcactcttt acgcagcgcg gtcaccggat ccatgcgcgc ggcgcgccag gcagggagga
      721 aactcgccac cgtgcagaaa aggatcaccg agagggccac gtcggtcacc tcgcgcggaa
      781 tcacgcgcca agggatctgc gccagcccgt acacgtcttt ggggaacacc tccagcccga
      841 agcggccgag gagcgacacc agaccctgca agttttccag aatcagcagg gccgcgccaa
      901 tgccaagcgc cgtgccgatc aggcattgga tccagccgtg cagcacaaag gccatcatca
      961 cctggcgcga ggaaaatccg agcgccttga gcaggccgat ctcatcggtc ttctgcacgg
     1021 tcagcacgat cagcgtgttg gtcacgcaga agatcgccac cacggtgatg aacatcagca
     1081 ggatcaccat catgtttttc tcgaccgcca gcgcattgaa aagctggctg tcgatctcct
     1141 gccaggtccg cacactgtag atcggcagca gcgcgcggat ttcgctcacc agccggctga
     1201 accccacggt gttttggggg tccgcgactt tgaggtggat cgaatagacc ccggatctca
     1261 tccccatcag gtcgcgcacc actgccagcg aggtgatcat gaatcccgaa tcgaaatccc
     1321 gctggcccga gttgtagatc cccgtaacgg tcagtcgttc cggaaaaaag agttcatccc
     1381 gggagacgag attcatcggc gagtagacca gcaggtcttc acccacccgg attcccaatt
     1441 cagcggccag atcgatcccc agcaccaccg catcgccttc aagttcgaac gcgccggccg
     1501 ccaagcgatc cagcttcatc acatgttgcg cccgggcggg atccgtaccg ataaggagcg
     1561 gagccaccac acggcggcgg tgttcgacca gcacccgcgt ctcgagtccc ggcgagactg
     1621 ccgtgacccc cggaacctgc tccagcctac ggcaaatctc cccttctcct tcgatcaccc
     1681 cgccgttccg ggacaagatg gtgatgtggg gcttgaagtc gaggatcttc tccttccaca
     1741 tgtctccgaa accggtcatc accgcgcgga cgatgatgat gatcgccacg cccagcacca
     1801 caccgatcac ggaaatcaat gtcaccaccg aagtgaatga acgcttcggc ttgaggtatt
     1861 tcaacgcgag aaagagagag aagggcatgg gggctgtcag cgcatacgcg cgcgagttca
     1921 tgagtgcatg agttcgcggc aggaagaaaa aacgaacgca cgaatgaact acaagtttcc
     1981 ggtgtcgact tggatgtccc ccgtgtccgt ggtggaggcg ctgatttccg cttcaacctg
     2041 aaccgcctct ttactggtgg tgggcagata gcggtaatag accatgagct gcaacgcagc
     2101 gaggcaggta tccatcacgg gacggtccga gtgcgcatcg gtgttttccc accagccgat
     2161 atcccgcaag gcgcccttgt gatcctggat ggcatccttc ttcacctgtt gggccttgac
     2221 gtaattgggc ttcatctgct tgttccaact gtcccagcgc ttgccgcccg catggaattt
     2281 gcactgggtg gcatagtaga aatagtactg cggcgacgca ttaaagaact tctgctcctc
     2341 gggcgggagg gtctttatga cacgggcctt ggccacggac aacggcgtct gattctgctt
     2401 gagttgcgat ttctcgattt tggcgagttc gtcggcaagg atggtgtagc tggagaaggt
     2461 cggcacccac gaatccatca gatccaagga cttccgcacc tcgggttcgt tcgaggcacc
     2521 cagcaactgc atacagagcg tgccaacgct ggtcaggccg ccagcgccgg gaccagtgta
     2581 accgaacccg ccgttgggcc cggcgttggc cttgaagcct cggatcgcca gcttgctggc
     2641 tttttccagg ccatccacct gaagacgcgc cagttgggcg gccttgatcg cctgcgcgca
     2701 ccatcccatg taagaagtgt catcacggta tttgccggtc tccttatccg gattgggatc
     2761 catatgataa gtccaaccgc ccgtgggatg ctgccccttg atgatcggga ccatcgccat
     2821 ttccgcggcc gtcttgacat tggggttgat ggtcataccg taggcttcgc aaagcgcgta
     2881 cgcggcgatc gggtggtcat agttgcctgg caggcggccg gttttcttgt tctggacgcg
     2941 catcagatac tccagcccct tctggaccgt ctcgccaaac tcggcggagt cgccgggctt
     3001 ctcgccgtgc gacagaaacg ccaggatggc gagaccggtc atggcgacct tgttccggtt
     3061 ccacgaaccg tccgcctgct ggttcttttt gagccagcgg agcgcgcgca tcaccgcctc
     3121 ttccgtaaac gcatcgccgc cgaagcgcgc gagttgcgtg ccacgcgtac ccgtgctccg
     3181 ggtggtgccg tagatgtttt tgagaatcac ggggcttttg accatcatca ccgcgtcgaa
     3241 ggcctgcggc tgggggctca ccggttggtc tgtcggcata tctgtgggca caggcacatt
     3301 cggattggcc acggggatat cgatgtcgac ctcctccatc ggctcttccg gcctctcaag
     3361 ttcttcttgg accttatcga ggtctttgat ctcctccgct tccagcacct gcgtctcgat
     3421 caggcggtcg ggcccggtcg cacccccgtc catcaccgag agcatcacaa ccgcgaacac
     3481 gggcagcaga atggcggtga gcggcgcgga gaggcgctgc agctcgatca tcgccatctt
     3541 gtactccctg gagtggcgcg gcgccttgag cccgctgaac agcttcttca ggcggcggag
     3601 gtaggaatct tccgtataca tctcgataat ctctccgaga cgggagttca cctcattcat
     3661 gtccagatag ctactcatgt cagactcctt atcacatcat cacatactga aaatcgacag
     3721 gttggtcatg ccatgcttgt tacagacatc cagcacctga atcagcatgg cgtgggtgga
     3781 gtcggcgtaa cacttgatga tgaccattgc ggttttgctg tatcccgaca acctctcgat
     3841 gctccggtcc agttccggca gccgcatggg gcggccgttg aacacaaagc cctgcggcgc
     3901 cacgatcacg ttgatcagat cctgctgctg ctccttaacc ttttcgtccg ggttcgccgc
     3961 agggcgggcc gccgagagtt tcgagaggat gtcctcctgc ttcagcgtga cgacgaagaa
     4021 gatgaggagt tggaagacca cgtcgatcat cggggtcatg tccagtttcg ggctttcgcc
     4081 tttgtttttt ttccgtttgt atgccatagg aaaaactcct gtcgctaccg gtcgtcccgg
     4141 gccttgtgtt cctgaaccgc cacgaacgaa agcttccaca cgccgcagga agtgcagaca
     4201 tccatgagtt tacggacttt ctcatgcaac gtgcgccggt cggcacgaat caacaccgga
     4261 aactcgttgc cgtgtttatt gacacgcgcc ctcaggatgt cacgcaagtt cttctctgtc
     4321 aacgtggcgt tatggatcga gacacgcccg cggcgatcca gttcgatctc cagcgtggtc
     4381 ggaggcatgt tctcggccgt gatggtgaca ccatgcttgc cgtcctccaa aacaatatcc
     4441 tgattgaatt cctgattcat cttcatcgtc accacaaaga agatgatcaa ctggaacacg
     4501 acgtcgatca ttggcgtcat gtcgatttcg cattgttcgt attctttggc catttttcaa
     4561 gatccgattt gcggggtaag ccgcacgtgt ttccaactcg ccacaggacc ggaaagatga
     4621 ccggccatcc cgccgcagaa agatcggcaa aagccgtcct cctctagccg tacagaaaat
     4681 ccggacggct aaggatgaca cggacattaa tcgctcacaa cctcgacgtt acgcaagtct
     4741 ttgatcaact ccatggtcat cgcctgcatg cgcagaatca gccggttcgc attgttacgg
     4801 atactgtagt aacaggtgat ggcgggaatg gcaatcgcca gaccgcccgc cgtcgtccaa
     4861 agagcctggg caatggcgag agccagcgcg gcaacgtccg gagcacccgt ggccaacgtg
     4921 ccgaagcaca tgatcatgcc ctgcaccgtt ccgagcaacc cgagcatcgg cgagaggttc
     4981 gagcacacgg agatccaggt gagccgctgc atgatgcgct cggtctccag gtccgcggcc
     5041 gcgccgatcg attcctggat cacctcaaag ccctcctcca catgggagaa ccctgcggag
     5101 aggatgttgg ccatggggcc gggctcgctc tcgcaagcgt gcagcgcctt catcacatca
     5161 ccctcagcca tggcgccctt aacctttgcg atcagtgcct gcggcatgat tttctgcggg
     5221 cggatcagga tggagcagtc gacgatgaaa tagatcgccg ccgcgccgca cccgaagagc
     5281 gcgagccaca ggacggcacc caaccaaccg gatcccaaga ccaccgcaag aaatccggtg
     5341 ctgtccttgg atgatttttt cgcgctctcg gtcggctgcg cgctttccac cgccgccggg
     5401 gccgcagccg ccgcagccgc cggggccgca tcctgagccg acactccgga ggcgcccatc
     5461 atcaaacctg cgaccaacac tgctgacatg accagtcttt tcatcattaa cctctgcttg
     5521 tttcccttac tgttctacca ttccacccgc acccctccgg cacgggaaat cccggacgcg
     5581 gcccctaccg tttgtgcgcc cactccgatg agccgtactc gctcttcagc gcggtccgca
     5641 tttgatccgc gcgcgaggac tgcccgaggt tatcaaaaca tttggcggcc ttgtacagcg
     5701 cttcaggctg cacgtccttt tcactcttat agagggtgat cacgcgcaga aatccatcgc
     5761 gtagcgcctt tttggcgttc tcgttggttt cgcccccggc cagaatgaca tcgccgcgca
     5821 tgatcagcgc gctcgccgaa gccgcccggt cgccgctctt gatcgccttg gccatcaggt
     5881 cctcgacttt ggtgacgcgg cccgatttca gaagcgcctt ccagtaggcg ggcgccatct
     5941 cgcccagata cgcggcctcg ggattggcgg caatgacctt ctcgcacacc tggcgggcct
     6001 tgtcggcgtt gccggatgcc acgtacgcct cggccagcag acgggtggcc ggtttgtccc
     6061 actggagcat caggtaatca ttggccagtt tctccagcac cgggatcgcc gccgccgcat
     6121 tcccctgatt catgttggcg atcgccgcgt ccaactcttt gggccgcggg atttgcacat
     6181 cgctgatgtt ggcgggggac atctccaact cgacgttccc gcctttcgcg gtgatcgcat
     6241 aggccttctc gcggactttc cacttgatca caccctcgat tttccggcct tcggaggtgg
     6301 tgatcacgcc cgcgatctgt gccgcggcgg ttcccgcggt cagcatcgcc agggcgaccg
     6361 caacccttcc ggtctgtgtg ctcatcttca ttcgtttgct ccttaaagaa cgttgcctca
     6421 ctgtccgatt ttcgcctggt tgagccagtt gcggatgtcg gtggcgtagc gcccgttggg
     6481 gaagatgcgc aggtaggtct cgcagtcctc ctcggcatcc ttgaacttct tatgctcgag
     6541 cagcagcggc acgcagtaga aataggcctt ctccagcgtt gacgccaatt tggcgttgcc
     6601 ggggttgatg ttcatgatca tcacctgaaa ggccacgatc gccttgccgc gcgtctccgc
     6661 cacctgctcc ttgagcccga gcttcttctc cgcctccatc ttgcgcaaca ggatttgacc
     6721 tgcggcgagg tccaactcga ccatctcctc cggctctttg cggtatttct tgaccatctt
     6781 gagcgcatcg atcgccgtgt tgaacagacg ggtgcgctcg ttgtcgtcct tctcgtgcct
     6841 tccctcttcg ctggccacct cgaccagcaa catgttggcg tccaccacga tttccagatt
     6901 ggagagcgct ttatcctgaa tgaacgcgtc cagcgcctta tgggcctcct gatacttctt
     6961 ctgcccaacc agcacgcggg cacgcccgag tttggcgttg gccaccaccg gtaggtcttt
     7021 ggccgtggcg agcacccggt cataggcctg cagggccatg tcatactccc gggcctgctc
     7081 gagcgcctgc gccgcggcca tgaactgccc ctcggtgtac tttccgcccg cgccgaacat
     7141 ctcacggtac ttggccacac cctcaccgcg caggcccatc tcgatcagac tggcggccag
     7201 caacggcacc gaattcctgg cctcctcgga ctgcgggtac tgcttgctga gtttctcgaa
     7261 agcggcctgc gcgttggacg catcctccag aatggtatgg agggtgccga tctgcaactg
     7321 cgccctgggg gccagtttgc cctccggata ctgtttgaca tactcctcga aggcggcaat
     7381 ggccagcttg cggaactgcg tcaggcgctc cggagggaag ttgacctgcg ccagacaaac
     7441 cgccttggtg aagaccgaca tctcgagtat ctgcgcgttg cgctgtttct cttcggcatt
     7501 gttctgatat tcgccggcct tttcgcgcaa cagcttggcg accccgtcaa accccaccgc
     7561 ggcgcgggtc agccagagca cagcctcctt ctgggtcgcc tcggcctgct cattggtgac
     7621 ggcggcgcgc atcagcgcgt tgccgtactc gcgctgggcg tccgccaaac ggaacttgcc
     7681 gaccaccagc ggattgcccg gccggtcgcg ctcggcaagc cgggagacgt acacttcgag
     7741 cgcctggatc tcattggtcc gatccccctc ttcgcgataa acctgggcga tgcggttcag
     7801 ggcgtcgaag tagtaggtcg agtgctgata ctgatccgtg atgagctggt agtacgtgag
     7861 ggcgccagtg taattcttgg cgtggaactc gcgctcgccg aaactcatca ccagctggcc
     7921 tgccgcgtaa tgggtggggt agtcgcggaa aaagagcgca taggtctcac gcttcttgtc
     7981 ctccatcgcc atttcgccga agcggtcgcc aatgcggcgc acctgatccc ccgcctcctt
     8041 catcatctct tccttggcgc agaagcgctc cgagaggtgg ccggtcaccg tgtccgccat
     8101 cagcagcgcg tccggatcct tccccgcagt ctcgatataa ctgatggcca gatcgcccag
     8161 cgccggcacg gactcggggg tctcggggaa ctggttgagg accagcaggt acttatcgat
     8221 ggcctcttta aactggctct ggctgaaaag caaccgcgcc ccggcgaatt gcatctggcg
     8281 caccttggcc atctgctcga ccgtcaccgg cgtgcggata tccgtattgt accgctcttt
     8341 aatcagcttg cggagggctt ccgagcgctc accggcctcg gccgcccact ggctctccgg
     8401 ataccggatg aacacattga tgaagtggtt gaaggcgccg ttgcctttgc gctggcgcgt
     8461 cttggggtcg cgctcgccca gcaacaggtc tttgatgcgg tcgtcgctgc cgccctcttt
     8521 ttgggcctcc gccacagcct cgttcatcag cagcaccgcc agcagatacc ggcactgcgg
     8581 catcgggctc atgcggagca tgcccagcga cccgtccgca tcctgctcga ccagggagtc
     8641 gtggatggtg cgaagctggg gcatgtagtt ttccaccaga tcctgcgccc ccttgacatc
     8701 cccccgcagg aggaagatgt gcgctttcat cacaatcgcc ttgccgaacc acacatcctg
     8761 cttccagagc agtttgtcaa ccgcggcttc ggcctctttg agcatggcgt ccttctcgcc
     8821 ctttttggac agattcggcg cgatcttgag catcagttcc gccatgtccg ccagaacctg
     8881 ccgctcgacc tcctcttcca acttcacgtt gaacaccctt ttgaatgcgg ccaaggcctc
     8941 agtctgcttg cccagacgga tgagcatctg cgcgtgcttg taagcggagt cacggtaaaa
     9001 agaggtcagc gcggccggcg gcttgctgaa ggttttgaag aaggcgaggt aaagcttgtc
     9061 cgcatccatg tacttgccaa aggcgtaata ggcatccgcc atggccagat tgagcgccca
     9121 gtactcctcg ccgttcttgt ccggaagggc ttccacgact ttccgcacct cgtcgaactt
     9181 cccctgccag agcaatccct gaaactcccg gatcttaaac tgggcggccg cttcgggaaa
     9241 acgctttttg gcttcggcga tcacttccgc agccagatca ggcatttgca actgctgcag
     9301 ggcgtcaatg tacttcaact cctcccgcaa ctcctgattg tccacatccc cttgcgcccg
     9361 tgacaggaac gcggacagga caatcgcaca ccacaacagc gcgagcccat gacccgaaat
     9421 catgcgaaag cacttctcat tcataatgcc catgttatac ggcaatttgg tttcccggtc
     9481 aatttcaaag aggcggtccg tgagaaaaat aatgcgcggc cgccccaccc tcaaatcggc
     9541 ccgccggaaa caaaaaagag tcttgttcat tttgttgttg cagtcgcgcc ccgttttgct
     9601 ttaattttga ctctatggcc gatcgtgtca caagcaaagg ttgaggttct aaaaatgggt
     9661 aagctgctgc gtgtattggt tgtcgtgatc ctgctcctga gtatcgtctc gctggttttc
     9721 gcaaacctgc tgttcggtaa gcgcgagctg ctgatccaac ggaattctgc tctggaggag
     9781 catgtcatcc gcctgtcgaa gacgctcgag gcgtccgacc ctcaggatgt ggcggccccg
     9841 gatgtccaga aggataactc ggacgtcacg gaccgcgaaa tcgcgaatcc ggaccgggtc
     9901 tcggttctgg agaactatcc gatcaagctc gaacagcaga atctgcccac cctggatttc
     9961 ggcagcacgg aaaaacggat tcagctccgg agctattacg ccctgacctc ggagggtaaa
    10021 atcgacccgg acccggtgga ccagaggccc cgcaccaaag gaccgggcac gatgcaggaa
    10081 ctgctcgacc tggcgtttga gcgcgccaag gtccagcagg ccacgctgaa caagacccgc
    10141 gccgaactca ccaaaatgcg cgagcgggtc accggcgttg tcgaggaggt caacaagctg
    10201 aaggccgaag ggcgtacagt caaacgcgac ctgaagggcg cccgcgaaca ggtcgccgcc
    10261 cttgaaaccg agaaggggga actcaatggc cgcatcacca aactcacggc cgaaaagcgc
    10321 gaactggccg ccgaggctgc tgacgccaag gatcaggccg aacgcctgaa tgaggaaaag
    10381 ctcaccctca ccgaggatct caccaagacg cgcgaggctc tggaagagat gaagaaacgc
    10441 ttcgcgggtg cgggcagccg cccggttcag gagagttccg gcgtcatggt gaccgccctc
    10501 tccgccgggg acaaaggcaa aatcctcgag gccaacgacg agttgaagtt cgcgattctc
    10561 gaattcaccg atgacgccat gaccgagatg ctcggcccgg aacgccagaa tgcgatgccg
    10621 cagttcgaaa tgaatgtgcg ccgcatcgga cgccagagcg cttccggtga gttcgtcacc
    10681 cgaatcaagt tgcgccaagc cgtgcggggc aaaaatctgg tggtcgccga catcctgagc
    10741 gactggcagc aagccccggt ggagaaaggc gatgtcgttt tcttctaagg ccgtacgggc
    10801 gtctctgggg ttggccctgc tgccgaccct cgccctgctg ctctccgggt gcatgtccga
    10861 ccagccgacc gaaacggaca tgccttgggc ggcccccgcc gcatgggagg gcaccatgcc
    10921 gctcccgggc gggttcatga accggtacga ataaaggcga aggccccttc tgaatcagag
    10981 ggggccttct tgttttcttg atcggctctc ggcttgaggc attgctgtcg gtttcgtccc
    11041 gttcacgttc agggaaacag gaatgcgtgc ccctcccggt tgttttccga aacagcggtt
    11101 gacgccgatg tataaacggc gtatgctt
//
LOCUS       DAOSRB010000089        11056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5806_length_11056_cov_7.176893, whole genome shotgun sequence.
ACCESSION   DAOSRB010000089 DAOSRB010000000
VERSION     DAOSRB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5806_length_11056_cov_7.176893"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..709)
                     /gene="purN"
                     /locus_tag="PLZ74_06280"
     CDS             complement(83..709)
                     /gene="purN"
                     /locus_tag="PLZ74_06280"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOR97964.1"
                     /translation="MLTPTLPLRIGVLGSGSGTNMQSVLDAIESGTLNAELRLVLSDV
                     QDAKILDRAARHKVPNAWLDCAPFKTKLDGEAEQRCIALLQAHGVDTVILAGFMRIVK
                     PGLLNAFPQRVLNIHPALLPAFPGLEGWKQALEYGVKLAGCTVHFVDAGTDTGPIILQ
                     RAVPVLTDDTPETLHARIQTQEHLAYPEALRLLAAGRLRINGRRVEVS"
     gene            854..1402
                     /locus_tag="PLZ74_06285"
     CDS             854..1402
                     /locus_tag="PLZ74_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOR97965.1"
                     /translation="MKCELCHKSDAETVLRKQVGDEVHEWFVCQACAQAAAKPPAPDR
                     NTRQTGEGGMPEASVLPLFGMIVDAAFEIADKQAMLTEPSCPVCGIRRSEYRKRSRLG
                     CPACYEAFADELDAALQELHRATRHTGRAPRNAEAACCRQELERALDEAVREQRYEEA
                     IALRDRLRALDGGQDAAPGGVS"
     gene            1399..2457
                     /locus_tag="PLZ74_06290"
     CDS             1399..2457
                     /locus_tag="PLZ74_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOR97966.1"
                     /translation="MKKLTPKRKTSANPVDGARAEEVALVCRVRLARNLEGERFPDWA
                     TEHDHARVFERVRSAFGAAAPDLALRPLSDFNVRYLEMLCEQNLISRDLLRRTSGGGI
                     AFEPPSAVCVMVNEEDHLRIQGFADGFDLNRAWQRATRMDALLEPHLHYAWSLRLGYL
                     TACPSNVGTGLRAGVMVHLLGLRLLGEIEAVMRGLERMRLLVRGIGGEGSEASGQIYQ
                     ISNMDTLGVEEEAIVGRVTRIVAEVIRQELNARERLLRESPLLLTDCLVRALTVLQNA
                     RLMSAAEALELLSAVRLGAVLGRFSKLKPVMVNQLILLMQPGHMQSGLQADLNPDQRD
                     EMRAAFLNQKLANVKWKG"
     gene            2463..5129
                     /locus_tag="PLZ74_06295"
     CDS             2463..5129
                     /locus_tag="PLZ74_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOR97967.1"
                     /translation="MDGFGNFTARAQRALQLAAREADRFNHPYIGTEHLLLGLIALGE
                     GVAVEVLEELDVSLQDVRIAVERMVGFGGETKTKGELPHTPRTKKVLHLAMAEAHAMR
                     QPQVGTEHLLLALLREGEGVAAQVLQSLHVRLEAVIEQIRLSMEAGEGDEEDGVFGPG
                     EGSGREEREARAAPDGKPGEGAGSKKERSRTPALNAFGRDLTELAKKGELDPMVGRAN
                     ELERLIQILCRRSKNNAALLGEAGVGKTAVVEGLAQAIALGDVPERMRDKRVVALDMA
                     LLVAGTKYRGQFEERIKAVVDEVCREKNIVLFLDELHTIVGAGGAEGAMDAANIIKPA
                     LARGELQCIGATTLNEYRRHIEKDAALERRFQTVRVDEPTVEETVEILKGLAPRYAAH
                     HNVTYEEDSLIAAAKLTARYQPGRQLPDKAIDAIDETGARVRMRVSARPPDLREHEQR
                     IREMGQKKDIAIREQRFEEAAALRDEERKARQELDAIVAAWKTEHAEKTLTVTADDVT
                     ETVARIAGVPIKRMSQSELSRLLHIENDLEEAVVGQTPAVQAIARALRRSRADLKDPR
                     RPIGSFIFLGPTGVGKTLLAKTLAEKMFGDDKALIQIDMSEYMEKFTVSRLIGSPPGY
                     VGHEEGGQLTERVRRRPYSVVLFDEVEKAHPDVMHMLLQILEEGRLTDSLGRQVDFRN
                     TVVILTSNLGFDYAKQGQGLGFGRESAADDYERLRERMIDSAKLVFKPELLNRFDEII
                     VFRKLDRQDVVRILDIELAGVRKRLETKGICLEITEEAAAFLVDKGFDPALGARPLRR
                     TVERHLEDPLAEELLRGVLTAGTVETTLKEDGTGLHFRMQDELPLRTPDKKTASPPRN
                     RKKKKTESATTVDAGTRPAPKPRKTRKRGKAE"
     gene            complement(5176..5304)
                     /locus_tag="PLZ74_06300"
     CDS             complement(5176..5304)
                     /locus_tag="PLZ74_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97968.1"
                     /translation="MVIFRTGTVAQPSITNGTAFVDDLRVTVFEPVWPPTTLLKLF"
     gene            complement(5298..5672)
                     /locus_tag="PLZ74_06305"
     CDS             complement(5298..5672)
                     /locus_tag="PLZ74_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97969.1"
                     /translation="MRPRCQWAGIAVALILIGGRAESKTVNVFFAGGQSNAKAVWATA
                     IADGLKAGYGSSLVMAHTNHSGEGLANWFTDTSKINYSNDLFHLNGTSVLQTRMAEIA
                     AAGDEPVFRGDVRPVSAEFRLW"
     gene            5852..6874
                     /locus_tag="PLZ74_06310"
     CDS             5852..6874
                     /locus_tag="PLZ74_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR97970.1"
                     /translation="MERNGTRRGFLRMGACGASVWALSGGVRQAEGASTDKSFGHGSF
                     ALDGNRIRVFSPAVREPVKVWVAGDTHLHRDDARGEPFREYSSRMAGGYNRVRHVESG
                     ELTTPEASFEETLRRAKEARVDLLALVGDVFSFPSEAAIEWAQAKLDASGVPYLYVAG
                     NHDWHYEGLPGSSMALRSEWTEKRLKPLYQGQNPLMAVREIRGIRFVAIDDSTYEILP
                     EQRAFFREQVADNTPLVLLMHIPLYAPGRPVGFGCGHPDWGAAQDRHWKIERRPQWPA
                     AGHTAVTRAFRNEVFSAPGILGVFAGHTHVQSLDVVNGVPQVVTAANAGCGMVRAEFL
                     PAHNGH"
     gene            6941..7987
                     /locus_tag="PLZ74_06315"
     CDS             6941..7987
                     /locus_tag="PLZ74_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR97971.1"
                     /translation="MAKRKPVQKRVAIVGFGFMGRMHYGQWKKIRNAKIVALCDQNQA
                     QFTAPVQGGNVAGADTSMDYGDAVIYDDFDRMLKEARPDVISLTLPTPLHVPFTVKAL
                     KAGVSVLCEKPMALNAVDCGVMLRAAKQAPKGTRLMIAQCLRFWPSYVYLKDLIDSGK
                     YGKVIAASFYRFSAPPGWQKGVNWFMDESKSGGVALDLHIHDTDIVQFLFGMPRAVTS
                     RANFAKDGAMRYISTLYDVGGAAVTAEASWAMAPTLGFEAGYVVTFEKAVVVLDDKRG
                     KPLCVYPAKGKAFVPKLPKGDGYEYEIRWFMDLLCGRKVACVTTPEQSRDSVRIVDAE
                     KRSARTGRTVRLAK"
     gene            8046..8861
                     /locus_tag="PLZ74_06320"
     CDS             8046..8861
                     /locus_tag="PLZ74_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOR97972.1"
                     /translation="MLKCGKWPVAVCSWSFRKSVEEIGSVMTALGLEHINLAIAPALA
                     PDGEAYLDAVRKQGWTVTAGMMNYDYEDYSTLDTIKVTGGIAPDAHWPASGEAFARAA
                     QITAALGSQYIMMHVGFLDHTDNAYAKKFYDRVRALGDSAGEAGVTLLMETGQETAEE
                     LQRFVETLNHPNVLLNFDPANLILYNKDEPLPALRRLAPWIRHIHIKDAIRTTVPGTW
                     GSEVVWGEGQVNAFAFLNVLEEIGYEGAVAIEREAGEQRVRDIATAVRRLVAY"
     gene            complement(8864..10000)
                     /locus_tag="PLZ74_06325"
     CDS             complement(8864..10000)
                     /locus_tag="PLZ74_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOR97973.1"
                     /translation="MNVMRSPFVTLVLIAMLAFLALQFREMRQRAVSAPSSPATGSVP
                     MSARPHAPEAERPCPETDAIAVYKRVSPSVVAVANHALIRRGFFGFQLYEVPQGAGSG
                     FVWDKKGHIVSNYHVVHQASTLTVTFPDGSRYDAVLVGIAPDYDLAVLRINAPPEKLV
                     PVTVATSRALQVGQQVYAIGNPFGLDTTLSAGLVSALGRTITSMTDRKIYDVIQTDTA
                     INPGNSGGPLLSSSGRLVGVNTAILSPSGAYAGIGFAVPSDTVSRVVPQLIEKGRVTR
                     AGLGVYLLPDHVTARAGIAGVALYAVAERTPAARAGLEGIGVSRLGDLIFGDVIQAVD
                     GIPVGTIEELQAVLDPRRPGEQVRLTVARNGKRRDVTLTLIEEE"
     gene            complement(10050..>11056)
                     /locus_tag="PLZ74_06330"
     CDS             complement(10050..>11056)
                     /locus_tag="PLZ74_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97974.1"
                     /translation="VNFDYNGIFNRPLYAPYAKNVAEFFRSPECRAIYLRKAQYFKDL
                     GFADSPAVYCWELWNEINCCGSIAQYSEWSEWALAELKRMFPRQLTVNNLGSFSDVGA
                     YQMYDALAQEKGNDFMQIHRYLDTGACLDVCRASMDVVAASATRELLDRSPKPAVVAE
                     IGAVQANHAGPFPYYPFDKEGTLLHDAVFAAFFAGSAGCGQFWHWDHQYIDGNNLWWH
                     FGRFAKAIEGLDPVAEEMKPFYTESRRLRMYGLRGKKTSIVWCRDKVSDWRSELVEGN
                     HAQVLSDQNVPFRNCDLDCYLPWEDRRVTVKAPRLPAFRRSIVVRVPSTAVEGVVRPH
                     "
BASE COUNT         2154 a         3063 c         3794 g         2045 t
ORIGIN      
        1 cccgcggcat tcggcccgcg acgctcgacc cgcgatgctc ggcccgcgac gctcgacccg
       61 cgatgttcga tctgctcgtc cttcaggaga cctcgacgcg ccgtccgttg atgcggaggc
      121 gtccggcggc gagcaggcgt agcgcttccg gatatgcgag atgctcttgc gtctggatcc
      181 gggcgtgcag ggtttcaggc gtgtcatccg taagaaccgg aacggctcgt tggaggataa
      241 tcgggccggt gtccgttccc gcatccacaa agtggacagt gcaccctgcc agcttgacac
      301 cgtactccaa agcctgcttc catccctcca accccggaaa tgccggaagc aatgcgggat
      361 ggatgttcag cacccgttgg gggaaggcgt tcaacaatcc cggtttcacg atgcgcataa
      421 accccgcgag gatcacggtg tcaacgccat gcgcttgaag cagcgcgatg cagcgttgtt
      481 ccgcctcacc gtccagtttg gtcttgaagg gggcgcaatc gagccaggca ttcgggacct
      541 tgtgccgtgc ggcgcggtcc aggatttttg cgtcctgcac atccgacagg accaaacgca
      601 actcggcatt cagggtgccg gattcgatcg catcgagcac ggactgcatg ttggttccgg
      661 aaccggaacc cagaacgccg atccgtaacg gtagggtagg ggtaagcatg tcggtagcgt
      721 aatacaatcc gtccggcgtg gcaaccggga agtcgggcgt ctttcgattg cactgaaaac
      781 gcgcgcaagg aggccgtgcc ggtttgcggt gcgtccctct ttctgctatt ctttttcttt
      841 ttcaggaaaa cggatgaaat gcgaattgtg tcataagtcg gatgcggaaa ccgtcctccg
      901 gaagcaagtg ggggatgagg tccacgaatg gtttgtttgt caggcgtgcg cgcaggcggc
      961 tgcgaaaccg cccgcgccgg atcggaacac ccgacagacc ggagaagggg gtatgccgga
     1021 ggcttcggtt ctgccgttgt tcgggatgat tgtggatgcc gcgtttgaaa tcgcggacaa
     1081 acaggcgatg ctcacggaac cgagttgtcc ggtgtgcggc atacggcgga gcgagtaccg
     1141 caagcgctca cgtctggggt gcccggcttg ctatgaggct tttgccgatg agttggatgc
     1201 cgcgctgcag gaacttcacc gcgcgacgag gcatacgggg agagccccga ggaatgccga
     1261 ggcagcctgt tgccgtcaag aactggagcg tgcgttggac gaggccgtgc gggagcagcg
     1321 gtatgaggag gcgatcgctc tgcgcgaccg gttgcgcgcg ctggacgggg gacaggacgc
     1381 ggcaccggga ggtgtgtcat gaaaaagctg acaccgaaac ggaaaacatc cgcgaatcca
     1441 gtagacgggg cccgagccga ggaagtcgcg ctggtgtgcc gtgtgcggct ggcgcggaat
     1501 ctggagggcg agcgctttcc ggattgggcc acggagcatg atcacgcgcg cgtgttcgag
     1561 cgtgtacgtt ctgcgttcgg ggcggcggca ccggatctgg cgctccggcc gctctcggat
     1621 ttcaacgtgc ggtatctgga aatgctgtgc gagcaaaacc tgatcagccg cgatctgctc
     1681 agacggactt cgggaggcgg aatcgccttc gagcccccaa gcgcggtctg cgtcatggtg
     1741 aacgaggagg atcatctgcg gatacaaggt ttcgccgacg gttttgacct gaaccgtgcg
     1801 tggcagcggg cgacccgcat ggatgcgttg ctcgaacccc atctgcatta tgcgtggtcg
     1861 ctgcggctgg gatatctgac ggcttgtccc agtaatgtgg gcaccggatt acgcgcgggc
     1921 gtgatggtcc acctgctcgg gttgcgtttg ctgggcgaga tcgaggcggt gatgcgcgga
     1981 ctcgagcgca tgcggcttct ggtgcgcgga atcggcggag aaggctccga agcctctggc
     2041 cagatctatc agatttccaa catggatacg cttggtgtgg aggaggaggc gatcgtcggg
     2101 cgcgtcacgc ggattgtcgc cgaggtgatc cgtcaggagt tgaatgcgcg cgagcgtctg
     2161 ttgcgggaat cgccgctgtt gctgaccgat tgtctggtac gtgcgctcac cgtgctgcag
     2221 aacgcgcggc tgatgtccgc agcggaggcg ctggagttgt tgtcggcagt acggttgggt
     2281 gcggtgctgg ggcggttttc caagctcaag ccggttatgg tgaatcagtt gatcctgctg
     2341 atgcagccgg ggcatatgca gagcgggctg caggcggatc tgaatccgga tcagcgcgat
     2401 gagatgcggg ccgccttcct gaatcagaag ctggccaacg tgaagtggaa agggtgaggg
     2461 gtatggacgg attcggaaat ttcacggcgc gcgcgcagcg tgcgcttcag ttggcggccc
     2521 gcgaggcgga ccggttcaat catccgtaca tcgggaccga gcacctcctg ctgggactca
     2581 tcgctttggg cgagggcgtg gcggtcgagg tgttggaaga gttggatgtt tcgttgcagg
     2641 atgtgcgtat cgcggtggag cggatggtcg ggtttggtgg cgagaccaag accaagggcg
     2701 agttgccgca tacgccgcgc accaagaaag tgctgcattt ggcgatggcc gaggcgcatg
     2761 ccatgcgtca gccgcaggtg ggaacggaac atctgcttct ggcactgctg cgtgaaggtg
     2821 agggcgtggc cgcacaggtg ctccaatcgc tccatgtccg gctggaggcg gtgatcgaac
     2881 agatccgtct cagcatggag gcgggggaag gagacgagga ggatggcgtg ttcggacccg
     2941 gtgagggttc cggcagggag gagcgtgagg ccagggccgc gccggacggg aaaccggggg
     3001 aaggcgcggg cagtaaaaaa gagcgaagcc ggacccccgc actgaatgcg ttcggacgcg
     3061 atctgaccga gctggccaaa aaaggagaac tcgatccgat ggtggggcgc gcgaatgagt
     3121 tggagcgtct gattcagatc ctttgccgcc gctccaaaaa caatgcggct ctgctgggcg
     3181 aagccggggt cggtaaaacg gccgtggtgg aggggttggc gcaggcgatc gcgttgggtg
     3241 acgtgccgga gcgcatgcgc gacaagcgtg tggtcgcgct ggatatggcg ctgctggtgg
     3301 cgggcaccaa gtaccgcggc cagtttgagg agcggatcaa agcggtggtt gatgaggtct
     3361 gccgcgaaaa aaacatcgtg ctgtttctcg acgagctaca caccattgtc ggtgcgggcg
     3421 gtgccgaggg tgcgatggac gcggccaaca tcatcaagcc cgcgctggcg cgtggcgagt
     3481 tgcagtgcat cggtgccacg acgctcaacg agtatcgacg ccacattgaa aaggatgccg
     3541 cactggagcg ccgctttcaa accgtgcgtg tggacgagcc caccgtggag gagacggtcg
     3601 aaattctgaa gggtttggca ccgcgttacg ccgcgcatca caacgtcacc tacgaagagg
     3661 attccctgat cgcggccgcg aagctgacgg cgcgctatca gcccggacgg caacttccgg
     3721 acaaggcgat cgacgcgatt gatgagacgg gcgcgcgggt gcggatgcgg gtttcggcgc
     3781 gcccgccgga tttgcgcgag catgaacaac ggattcgcga gatggggcag aaaaaggata
     3841 tcgcgatccg cgagcagcgc ttcgaggaag ctgcggcgct acgtgatgag gagcgcaagg
     3901 cccgtcagga actcgatgca atcgtggcgg catggaagac cgagcacgcg gaaaagacgc
     3961 tgacggtgac ggcggacgat gtcacggaaa cggtggcgcg gatcgcgggc gtgccgatca
     4021 aacgcatgag ccagtcggaa ctctcgcgcc tgctgcacat cgaaaacgat ctggaagagg
     4081 cggtggtggg gcagacccct gcggtacagg cgattgcccg ggcactgcgt cgctcgcggg
     4141 ccgatttgaa agatccgcgg cgtccgatcg gctccttcat ttttctgggg ccgaccggtg
     4201 tgggcaaaac cctgctggcc aagactctgg ccgaaaagat gtttggtgac gacaaagccc
     4261 tgattcagat cgacatgtcg gagtacatgg agaaattcac ggtctcgcgg cttatcggct
     4321 cgccgcccgg ttatgtgggg cacgaagagg gggggcagct taccgaacgt gtgcgcaggc
     4381 gaccctatag tgtcgtgctg ttcgacgagg tcgagaaggc gcatccggat gtgatgcaca
     4441 tgctgcttca aattctggag gagggccgcc tcacggacag tctgggacgt caggtggatt
     4501 tccgcaatac ggtggtgatc ctgacctcga acctcgggtt cgattacgcg aagcagggtc
     4561 agggactggg cttcggacgc gagagcgcgg cggatgatta tgagcgttta cgcgaacgaa
     4621 tgatcgactc cgcgaaactc gtgttcaaac cggaattgct gaaccgattt gatgagatca
     4681 tcgttttccg gaaactggat cggcaggatg tggtccggat tctggacatc gaactggccg
     4741 gggtccggaa gcgtctcgaa acaaagggga tttgtctgga gatcacggaa gaagcggccg
     4801 catttctggt cgacaaggga ttcgatccgg cactcggcgc gcgtccgttg cggcgtacgg
     4861 tggagcggca tctggaggat ccgctcgcag aggaactgtt gcgcggcgta ctgactgcgg
     4921 gcacggtcga gacgacatta aaagaggacg ggacaggatt gcacttccgc atgcaggacg
     4981 aattgccgtt gcgtacaccg gataagaaaa cggcctcgcc gccccgtaac cgtaagaaga
     5041 aaaaaacgga gagcgcgaca acggtggacg ccggaacccg gcccgccccg aagccgagaa
     5101 agacgcgaaa acgcggcaag gcggagtgat tcagaggcgg tctgcccggc acgcgagccg
     5161 ctttgattgt acgggtcaga acagcttcag aagcgttgtg gggggccaga ccggttcaaa
     5221 aaccgtgacg cgcaggtcgt ccacgaatgc cgtgccgttg gtgatggatg gctgggcgac
     5281 ggtgccggtg cggaagatca ccatagcctg aactccgctg acaccggccg cacatccccc
     5341 cggaaaaccg gttcgtcccc ggcggcggcg atctccgcca tgcgggtctg caagacactc
     5401 gtgccattga ggtggaagag atcgttgctg taattgatct tgctggtgtc ggtaaaccag
     5461 tttgcgagac cctccccgga atgattggtg tgcgccatca ccaaagacga accgtacccg
     5521 gctttcagtc cgtcggcgat tgcggtggcc cagacggctt tggcattcga ctggccgccg
     5581 gcgaaaaaga cattcacggt tttcgattcg gcccggcctc cgataagtat aagcgcgaca
     5641 gcgattccag cccactggca tcttggtctc atcatctttc ctttcgcctg cacaaccctg
     5701 tccatacgat acgggtggcg gggtaacgtt ccccccaacc cgatgtgttg catatcttcg
     5761 cagttcatct ccggggacgt caatctggac tttgcgttcg acacatcgct ctttttttcg
     5821 cacaatctca aaccgtaaag gaggtcgagt tatggaacgg aacggaacga gacggggatt
     5881 tctccggatg ggggcgtgcg gcgcttcggt ttgggcgctc agcgggggtg tacggcaggc
     5941 ggagggggcg tcgacggaca aatcgttcgg gcacggatcg ttcgcgttgg acggtaaccg
     6001 gattcgggtg ttttccccgg cggtccgcga gccggtcaag gtgtgggttg cgggtgacac
     6061 gcatctgcac cgtgatgacg cgcgtggcga accgttccgc gaatacagca gccgtatggc
     6121 ggggggctac aaccgtgtgc ggcatgtcga gagcggcgag ctcaccacgc cggaggccag
     6181 tttcgaggag acgctccggc gcgcgaagga ggcgcgcgtc gatctgctcg cgctggtggg
     6241 cgatgtgttc agctttccct ccgaggcggc gattgaatgg gcacaggcca aactcgatgc
     6301 gtcaggtgtg ccctacctct atgtcgcggg caaccatgac tggcactacg aggggttgcc
     6361 cggatcgagc atggccctgc ggagcgaatg gacggaaaag cggctcaagc ccctatatca
     6421 gggacagaac ccgttgatgg ccgtccgcga aatccggggg atccggtttg tggcgatcga
     6481 cgattcgacc tacgagatcc tgcctgaaca gcgggcgttt ttccgcgaac aggtggccga
     6541 caacacgccg ctggttctgc tcatgcacat tccgctctac gcgcccggcc gaccggtcgg
     6601 attcggttgc ggccatcctg actggggtgc cgcgcaggac cggcactgga agatcgagcg
     6661 ccgtcctcaa tggcctgccg caggccacac ggcggtgacg cgcgccttcc gcaatgaggt
     6721 gttcagcgcg cccggcattc tgggcgtgtt cgcgggccat acccatgtgc aatcgctgga
     6781 tgtggtcaat ggtgtgccgc aggtcgtcac cgccgcgaat gccggctgcg gcatggtgcg
     6841 cgccgaattt ctgcctgcgc acaacggtca ttgacaagct ggcgccgatt taggatgcta
     6901 tacgcacagt cgtcgtaagg ataaccgcaa gggaggagat atggctaaac ggaaaccggt
     6961 tcagaaacgc gtcgccatcg tggggttcgg gtttatgggg cgcatgcatt acgggcagtg
     7021 gaaaaagatc cggaacgcga aaatcgtggc gttgtgcgat cagaatcagg cgcagttcac
     7081 ggctccggtt cagggcggga acgtcgcggg tgcggacacc tcgatggatt acggggatgc
     7141 ggtgatttat gacgacttcg accggatgtt gaaggaggcc cgcccggatg tgatctcgct
     7201 gaccctgccg acgccgttac acgttccgtt cacggtcaag gcgctgaaag ccggggtgag
     7261 cgtgctgtgc gaaaagccca tggcgttgaa tgcggttgat tgcggcgtga tgctgcgcgc
     7321 cgcgaaacag gcgccgaagg gtacgcggct gatgattgcc cagtgcctgc gcttctggcc
     7381 gagttacgtg tatctcaagg atctgattga tagcgggaag tacggcaaag tcatcgcggc
     7441 ctccttctac cgtttcagcg cgcctccggg ctggcaaaaa ggggtcaact ggttcatgga
     7501 tgagtcgaaa agcggcggcg tggcgctgga tctgcacatc catgacacgg atatcgttca
     7561 gtttttgttc ggcatgccgc gcgcggtgac gagccgggct aactttgcga aagacggggc
     7621 gatgcgctac atctccactc tgtacgatgt gggcggtgcg gcggtgacgg ccgaggcaag
     7681 ttgggccatg gcgccgacgt tgggcttcga ggccgggtat gtggtgacgt tcgagaaggc
     7741 cgtagtcgtg ctggacgata aacgcgggaa accgctctgc gtctatcctg ccaaaggaaa
     7801 ggcgttcgtg ccgaaactgc cgaaaggcga cggctacgag tacgagatcc gctggttcat
     7861 ggatctgctg tgcggccgca aggtcgcgtg tgtgaccacg ccggaacagt cgcgcgattc
     7921 cgtgcggatc gtggatgctg aaaaacgttc ggcccggacg ggcagaaccg tgcgcctcgc
     7981 aaagtaagcg aaaaaagaga agcgaaaaca ttgggcccgt tcgccggacc cgcggaggaa
     8041 agatcatgtt aaagtgcgga aaatggccag tggccgtgtg cagttggtcg ttcagaaaaa
     8101 gcgtggagga gatcgggtct gtgatgaccg cgctgggatt ggagcatatc aacctggcga
     8161 tcgccccggc tctggcgccg gacggcgagg cgtatcttga tgccgttcga aagcagggct
     8221 ggactgtgac ggccggcatg atgaattacg actatgagga ttacagcacg ttggacacga
     8281 tcaaggtgac cggcgggatc gcgccggatg cgcactggcc cgcgagcggg gaggcgttcg
     8341 cccgtgcggc ccagatcacg gctgcgctcg gctcgcagta catcatgatg cacgtggggt
     8401 tcttggatca cactgacaac gcctacgcta aaaaattcta tgaccgtgtg cgcgcgctgg
     8461 gggattcggc gggtgaggcg ggcgtgacgc tgctgatgga gaccggacag gaaacagccg
     8521 aggagcttca gcggtttgtt gagaccctga accatccgaa tgtgctcttg aacttcgatc
     8581 cggctaatct cattctgtac aacaaggatg agccgcttcc ggcgctgcgt cgcctcgcgc
     8641 cttggatccg ccacattcac atcaaggatg cgatccgaac cactgtgccc ggcacatggg
     8701 ggagcgaagt cgtgtggggc gaagggcagg tcaatgcttt cgcgttcctg aacgtgctgg
     8761 aggagatcgg atacgagggt gccgtggcca tcgagcgcga ggcgggtgag cagcgcgtcc
     8821 gcgatatcgc cacagcggtg cgccgattgg tcgcctattg aggtcactcc tcttcgatca
     8881 gcgtcagggt cacatcccgc cgtttgccgt tacgggcaac ggttagccgg acttgttcgc
     8941 ccggccgacg ggggtcgagg acggcctgta gctcttcgat cgtgcccacc gggattccgt
     9001 ccacggcttg gatcacatcg ccgaaaatca gatctccgag ccgactgacg ccgatccctt
     9061 caagacctgc gcgtgcggcg ggtgttcgtt ccgcaaccgc gtacagggcc acgcccgcga
     9121 tgcccgcgcg tgcggtcaca tgatcgggca acagatacac gccgagtccg gcacgcgtga
     9181 cccggccctt ctcaatgagt tgcggcacca cgcgggacac ggtgtccgag gggaccgcga
     9241 agccaatgcc ggcatacgcg ccactcgggc taaggatcgc ggtgttgacc cccactagcc
     9301 tgccggatga ggaaagcagg ggaccgccgg agttgccggg attgatcgcg gtgtcggtct
     9361 ggatgacatc gtaaatcttg cggtcggtca tggaggtgat ggtgcggccg agtgcgctga
     9421 ccagaccggc cgatagggtg gtgtcgagac caaacgggtt gccgatcgcg taaacctgct
     9481 gtccgacctg caacgcgcgt gaggtggcga ccgtgaccgg cacgagtttc tcggggggtg
     9541 cgttgatcct cagcaccgcc aaatcataat ccggcgcgat accgaccagt acggcgtcgt
     9601 accgtgatcc atccgggaag gtaacggtca gtgtgctggc ttgatgcacc acatggtaat
     9661 tactgacgat atggcccttc ttgtcccaca cgaaaccgga gcccgcgccc tgcggcacct
     9721 cgtacagttg gaatccgaag aacccgcgcc ggatcagcgc gtggttggcc acggccacca
     9781 ccgagggcga gacgcgttta taaaccgcga tggcgtcggt ctcgggacag ggtcgctccg
     9841 cctctggcgc atgcggtctt gcggacatcg gcaccgagcc cgttgcagga gaggacggtg
     9901 cggacacagc gcgctgccgc atctcccgga actgcagcgc cagaaacgcg agcatggcga
     9961 tcaacacaag ggtgacaaag ggggagcgca tcacattcat gccattagta tagcgcagga
    10021 ctctgattgg attcgattca tttcactcct cagtgcggac ggaccacgcc ctcgacggct
    10081 gtggagggca cgcggacgac gatcgaacga cggaacgccg gcaaccgcgg cgccttgacc
    10141 gtgacccggc ggtcctccca gggcagatag cagtcgaggt cgcagttccg gaacggcacg
    10201 ttctggtccg agagaacctg tgcgtggttt ccctcgacga gttcactccg ccagtcgctg
    10261 accttgtcgc ggcaccagac aatggacgtc ttcttcccgc gcagaccgta catccgtaac
    10321 cgccgcgatt ccgtgtagaa cggcttcatc tcttcggcga cggggtcaag gccctcgatc
    10381 gccttcgcaa agcgcccgaa atgccaccag aggttgttgc catcgatgta ctggtggtcc
    10441 cagtgccaga actggccgca acccgccgaa cccgcgaaga acgccgcgaa gacggcgtcg
    10501 tgcagcagcg tgccctcctt gtcaaagggg tagtagggga aggggccggc gtggttcgcc
    10561 tgaacggcgc cgatctcggc gacgaccgcg ggcttcggcg agcggtcgag cagttcgcgc
    10621 gtcgcactcg cggccaccac gtccatcgac gcgcgacaga catcgaggca ggctccggtg
    10681 tcgaggtagc ggtgaatctg catgaagtcg ttacccttct cctgcgcgag cgcgtcatac
    10741 atctgatagg cacccacgtc agagaagctg ccgaggttgt tgaccgtgag ttgacgcggg
    10801 aacatccgct tcaactcggc gagcgcccac tcggaccatt ccgaatactg ggcgatcgag
    10861 ccgcagcagt tgatctcgtt ccagagttcc caacagtaga ccgcaggcga gtcggcgaag
    10921 ccaaggtcct tgaaatactg cgccttgcgg aggtagatcg cgcggcactc cggcgaacgg
    10981 aagaactcgg cgacgttctt tgcgtacggc gcgtagagcg gacggttgaa gatgccgttg
    11041 tagtcgaagt tcacct
//
LOCUS       DAOSRB010000090        11056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_5807_length_11056_cov_5.997182, whole genome shotgun sequence.
ACCESSION   DAOSRB010000090 DAOSRB010000000
VERSION     DAOSRB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5807_length_11056_cov_5.997182"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLZ74_06335"
     CDS             <1..1074
                     /locus_tag="PLZ74_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOR97975.1"
                     /translation="ERLEWSQRLAKANFLTRTELQADQLALQRKKLDLAMAETKMSVL
                     TNFTVLQQRATLESALRKATRALKRTTWQNKATLRQVETELQARQRELNRATNRMAEL
                     NFQIEKSKIYAPTNGIVLYTSTVLISRRQWWIKPLAVGSTAVERQELIYIPLESGMVV
                     EVMVPEASLNKIENGMEARVKVDAFPGRVFEGHLVKIGILPDGQSSQLNPDLKLYKCE
                     IECDFKEVNIRPGMSCDVELVKETYENALYVPVQCVVRVEGVPRVYVRDDGEWVPRIV
                     SVGMDNNRMIHIREGLKVGEEVMLAPPVKEQKKEAVEEAVPPGGAPARSENRNGSSAK
                     PRRASAPSPARRGEGPPAGGRMP"
     gene            1077..1796
                     /locus_tag="PLZ74_06340"
     CDS             1077..1796
                     /locus_tag="PLZ74_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR97976.1"
                     /translation="MQPTAWAASDPDAVVRLDMVKRHYRVGDETVRALDGVSFVIRRG
                     SYWAIMGQSGSGKSTLLNILGCLDRPSSGAYWLNGVNVAEMDDDSLSDHRLRNLGFVF
                     QSFHLIPQLTVCENIEVPMLYLGLSQTTRRAKAEQLAERVGIGHRLRHLPSELSGGQR
                     QRVAVARALANDPAVLLADEPTGNLDSATSVQIMELFQELYEQGKTVVLVTHEADIAA
                     YAKASIHLKDGKILSVTEAEA"
     gene            1793..3064
                     /locus_tag="PLZ74_06345"
     CDS             1793..3064
                     /locus_tag="PLZ74_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR97977.1"
                     /translation="MNQITYFWPFVKLGLTDVWRHKTRSFLTMLGMVFGVGSVIAMLA
                     VGEGASQQALESIKRLGSENIMVLSVKPGSEESGSSGAQRGKLAVYGLLYDDEKRIRE
                     TVPGVVMTVAARMTRRTARLNERQLEMRVVETVPEWFQVVQRPILAGRVLTAHDMRIR
                     ANVCVLTEHGVRRLLAAEETIGQRIRIASGVFEVIGIVQNETGGSVRAPDSEADAYIP
                     ITTGTKFFGEANIRYSAGGMEGEKVELSQIIVKMADTSVVESGSKAIQAVLERFHKKK
                     DFRIDVPLTLLREAERTKRTYNIVLGAIAGISLLVGGIGIMNIMLASVTERTKEIGIR
                     RAIGARRSRIVVQFLINTCVLSIGGGFVGLILGVAIPFLITLFTDMPTVVQPYSLVLA
                     FGISAGIGIVFGLYPAMRAAALDPIEALRRD"
     gene            3243..4295
                     /gene="sppA"
                     /locus_tag="PLZ74_06350"
     CDS             3243..4295
                     /gene="sppA"
                     /locus_tag="PLZ74_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOR97978.1"
                     /translation="MSDNRDTSKTGRGCGFGCALAIGMFIGFGLLVAVLVTGSFHVLG
                     VAGEHGAMEWFRSFRTRPEAGVDEAPNLIETWSAGDGEVKVVRIPLNGMIMLGDASWY
                     AGNAAAALRAIRKARHDPEVAGLILEINSGGGGITDSDILYQALCDFKTSSKGRVVVS
                     LLGDVAASGGYYVALASDVILAHPTTLTGSIGVLLQAYNIQELAAKIGIRDVTVKSGE
                     NKDLFNPFQEVKSTQREMVQKIITAMHERFVGLVAKNRTLPLETVRTLADGRIFLADE
                     ALRHKLIDGIGYQADAQSRIAQLLETDAVKVFRYEEQVTLMDLFTRRGIGLQTDLGRF
                     LRDDARDARLLYRWSW"
     gene            4309..6648
                     /gene="murC"
                     /locus_tag="PLZ74_06355"
     CDS             4309..6648
                     /gene="murC"
                     /locus_tag="PLZ74_06355"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOR97979.1"
                     /translation="MNVKTGEWRVPGRVHLLGIGGVGMAGLAYLLARQGWRVSGCDAR
                     PNALCRWLEEAGIPVAGKHAPEHLDEAERLIVTPAVAPDEPEWVQARARGMPVFFRGE
                     VLAAWVSSRRGVAVCGAHGKTTTACFATRLLGMLGPSPAWCVGGATRAMGKVAWEGAE
                     GQEVFVAEADESDGTLALYRPAVTVVTNMDLDHLDHFANEEALLNCFRAVAAQTREGV
                     AFCRDDPRATLAAQAATVPVLGFGFGEAADLRACAVEAGAESVAFTPVYRGREQARVV
                     LCVTGRHNVLNALGAAAAALILGCDPAAVFAALPQACGELPGRRFECVSDEGGIRVVT
                     DYAHHPVELEAAVAMARLQEPSRLVVVFQPHRYSRTKALAHAFPQALEGADEVILLPV
                     YAASEPWIEGGDSADLYAHFREAGMGDTRVMLARSQEEAWQFLRQTLQPGGLLLLAGA
                     GDVIGMVDGIREDRQRGWPACRDPEGFEKRVRAIEGLHVTPFGTLSEWSFFRTGGWAR
                     WRVEAADETALGALLKQCRQDDVPWRFVGAGANSWFSDLGEPGCVIRLAASACREWET
                     AGDEIRVGCNWKGAALLDELERRGLTGLEFLDSVPGSLGGWLAMNAGAHGGEIGERVR
                     WIRCLNSDGEITILHPMDFGFGYRRCGGLTGCIALSCALRVERADAGAVRVRREAFRA
                     RRISLAGLRTEGSVFRNPPGDAAGRLLDTAGCKGMRVGGARVTARHANIVAVEDPVTA
                     SDVLALVTRMRRRVEQVWGVSLSPEICGLTEGVTEEGIG"
     gene            6645..7541
                     /locus_tag="PLZ74_06360"
     CDS             6645..7541
                     /locus_tag="PLZ74_06360"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOR97980.1"
                     /translation="MKRIAVMMGGTSSEREVSLRSGEAVVAGLREAGFDAVPVVLNAD
                     RIDTLPSAVDAVFLALHGGYGENGGVQSDLDRLGLPYTGPGAAASRIAMDKIATKRVL
                     EAAGVPTPAFEVLSRGMEDFSLPLPLVVKPPRDGSSVGVVKVTSADAWPAALEQARAK
                     DPRGEVLVEAYIDGREWTVSVLDGEALPVVEIEAPGGWYGFTEKYTSGMTRYTFPEAV
                     EDAPLAAQCRVLALLAFQAVGCRGVSRVDFRVTSSGEAYVLEINTIPGFTATSLLPKA
                     AAKAGLSFSELCARLIGTATCG"
     gene            7538..8434
                     /locus_tag="PLZ74_06365"
     CDS             7538..8434
                     /locus_tag="PLZ74_06365"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOR97981.1"
                     /translation="MRRGTKRMAWDFFKQGGTQRKRMQAKGRRVTGVVTDLPPGVRFA
                     IRVAAGMVILLVVTWGGWRLFEAYYFCSRRLFVLHNLRENVMITTGRTLTPDLICEVL
                     GLREGVNLFSIPIRKKRKELLEQAPNIRDITIVRRMPDKIRINIIEREPIARVGSNGR
                     VVDEEGVVFIRYAGTGGLPMIKGVDAFEQIKPGDRLRGNELAAVRLVNNAQRPECRLR
                     LLVVDTSKEDYLLLTLFDHRQVKFAWKGMRDEAKDTVSRMQRHFDQLAQAMESEIGRA
                     CLMWDATQPGRIFATPSGLNEP"
     gene            8453..9655
                     /gene="ftsA"
                     /locus_tag="PLZ74_06370"
     CDS             8453..9655
                     /gene="ftsA"
                     /locus_tag="PLZ74_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOR97982.1"
                     /translation="MAIPPIAALEIGTSRTVLCVGESDDRGRVKITGVGTYPTTGVRK
                     GQVFDLAQARVGVELAAKQAEKQADVSIWQVLLAASGGHIQAVANPGMVPIRSGDRVV
                     TQEDVEEVTENAQTIQLDADRQVLHTINQSFTVDDQPGIVKPVGLRGKILTLNVMAVH
                     GLRNHIENAISVAKSAQLEVTDVAFSGICAGLAVLTPEQRRRGVVLIDLGGGTTNYLA
                     YGNGVVSALGCVAVGGDHITNDIALAFTIPLNRAEELKCAEGCALIEPESGTRRITLS
                     ADIGFEERVLSCKALHTVIHARMDETFRIVRSKLDEGGVLPHLGAGVILTGGGAYLRK
                     ATDLAQRIFGLPCGIGLPVNVSGLDNVPQAAALSTAAGLVLYGRMTYEDRGILSPLKN
                     LFKGVFRR"
     gene            9676..10827
                     /locus_tag="PLZ74_06375"
     CDS             9676..10827
                     /locus_tag="PLZ74_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97983.1"
                     /translation="MLLMGIGGGGCRLAAMVQSTYGDGIRALGMDTDALSIRTASEGG
                     LTCLLLGGSRLTGHGTGGDAIMGRLAVQDDLPNLTAHIQGVRSVVLVTCLGSGTGGGA
                     TPEVVKALHSQGIATLCFVTLPFAFEGENRRKAAERVLPMIEEHADSVVVLPLDDLFA
                     DTREPVLATALEAADRVLATAVTLLWRLVSTPGFIALDEERLHAMVVKGGNARFGFAS
                     AAGPDRALRAAEMLRECRTLRHGDALSKANALLFGILAGRDLRLAEIGDLMERMRACC
                     KKECVVEMGTVLDPDFEGRVELVALAFESWTAASLVAPIPDVPAGHEPPVADSFPIRP
                     GGRRSRSKGSKLSFGATGRGKFDQVEPTLHSGQDLDIPTFMRRGITLER"
BASE COUNT         1907 a         2916 c         4064 g         2169 t
ORIGIN      
        1 gagcggttgg agtggtcgca gcggctcgcc aaggcgaact tcctgacccg gaccgaactg
       61 caggccgacc agttggccct gcagcgcaaa aagctcgatc tcgccatggc ggagaccaaa
      121 atgagcgtgt tgaccaactt cacggttttg caacagcgcg cgactctgga aagcgcgttg
      181 cggaaagcca cgcgcgcgct gaaacgtacc acttggcaga acaaggctac gctgcgtcag
      241 gtggagacgg aactgcaagc gcggcaacgg gaactcaatc gggccaccaa ccgcatggcg
      301 gaactgaact tccaaatcga gaagagcaag atctacgcgc ccaccaacgg aatcgttctg
      361 tacacctcga ccgtcctgat ctcccggcgt caatggtgga tcaagccgct ggcggtgggt
      421 tccaccgcgg tggagcgtca ggagctgatc tacatcccgc tggagtccgg aatggtggtg
      481 gaggtgatgg ttcccgaggc cagtctgaac aagatcgaaa acgggatgga ggcgcgtgtc
      541 aaggtggacg ccttcccggg gcgtgtgttt gaggggcacc tggtcaagat cgggatcctg
      601 ccggatggcc agagttcaca gttgaatccg gatctgaagt tgtacaaatg cgagatcgag
      661 tgcgatttca aggaggtcaa catccgtccc ggcatgagct gtgatgtcga actggtcaag
      721 gagacgtatg agaacgcgct ctacgtgccg gtgcagtgcg tggtccgcgt ggagggcgtg
      781 ccgcgcgtct atgtgcgcga tgacggcgaa tgggtgccgc ggatcgtaag cgtgggcatg
      841 gacaacaacc ggatgatcca catccgggaa gggctgaagg tcggcgagga ggtcatgctg
      901 gcgccgccag tgaaggagca gaaaaaagag gctgtggagg aggcggttcc tccgggtggc
      961 gcgcccgcgc gttcggaaaa ccggaacggc tcgtccgcca agcccagacg agcctccgcg
     1021 ccctcgccgg cgcggcgtgg ggaggggccg cccgcaggcg gcaggatgcc atgatcatgc
     1081 agccgacagc gtgggcggca tccgatcccg atgcggtggt gcgcttggat atggtgaaac
     1141 ggcattaccg ggtcggcgac gaaacggttc gggctctgga cggcgtttcc ttcgtgatcc
     1201 gccggggttc ctattgggcc atcatgggcc aaagcggttc tggaaaaagt acgctgctga
     1261 atattttggg gtgtctggac cggccttcgt cgggtgcgta ttggctgaac ggtgtgaatg
     1321 tggcggagat ggatgatgac tcgctgagtg atcatcgtct gcggaatctg ggtttcgtgt
     1381 ttcagagttt tcatctcatt ccgcaactga cggtgtgtga aaacatcgaa gtgccgatgc
     1441 tgtatctggg gctttcgcag acgacgcgca gggcgaaagc ggagcaactg gccgaacgtg
     1501 tcggcatcgg gcaccggctg cgccatctgc cttccgaact gtcgggcggg cagcgccagc
     1561 gtgtggcggt tgcccgcgcg ctggccaacg atccggcggt cctgctggcc gatgagccga
     1621 ccgggaatct ggacagcgcc accagtgtgc agatcatgga gttgtttcag gagttgtatg
     1681 agcagggcaa gaccgtggtt ctcgtgacgc acgaggcgga catcgccgcc tacgccaaag
     1741 ccagcatcca tctcaaagac ggcaagatcc tctcggtcac ggaggcggag gcgtgaacca
     1801 gatcacctat ttctggccgt tcgtgaaact cggtctcacc gatgtgtggc ggcataagac
     1861 ccgctcgttt ctgacgatgc tcggcatggt gttcggtgtg ggcagtgtga tcgcgatgct
     1921 ggcggtcggt gaaggggcca gtcagcaggc gctggagtcg atcaagcggc tgggcagtga
     1981 aaacatcatg gtgctctccg tgaaaccggg gtccgaagag agcggcagtt ccggcgctca
     2041 gcgcggcaaa ctggccgtat acgggttgct gtacgacgac gagaagcgca ttcgtgagac
     2101 ggtgccgggt gtcgtgatga ccgtggccgc gcgcatgacg cgccggaccg cgcgtctgaa
     2161 tgagcgccaa ctggagatgc gcgtggtgga gactgtcccc gagtggtttc aggtggtgca
     2221 acggccgatc ttggccggcc gggttctcac ggcgcacgac atgcggatac gcgcgaatgt
     2281 ctgtgtcctg accgaacatg gcgtgcggcg gttgctggcg gccgaggaga ccatcggcca
     2341 gcggatccgc atcgcttcgg gcgtcttcga ggtcatcggc atcgtgcaga acgagacagg
     2401 cgggtcggtc cgcgcgccgg acagcgaggc ggacgcgtac atcccgatca ccaccggaac
     2461 gaaatttttc ggcgaagcca acatccgcta ttcggcgggc ggcatggaag gcgagaaggt
     2521 cgaactcagc cagatcatcg tcaagatggc ggacacctcc gtggtcgaat ccggcagcaa
     2581 ggcgattcag gcggttctgg agcggttcca caagaaaaag gatttccgga ttgatgtgcc
     2641 gcttacgctg ctgcgtgagg cggagcgcac caaacggacg tataacattg tgttgggggc
     2701 gatcgcgggc atcagcctgc ttgtgggcgg gatcggcatc atgaacatca tgttggcgtc
     2761 ggtgacggag cgcaccaaag agatcgggat tcggcgggcg atcggcgcgc gccgcagccg
     2821 gatcgtggtg cagtttttga tcaacacctg cgtgttgtcg atcgggggcg ggttcgtggg
     2881 gttgattctc ggcgtggcga ttccgtttct catcaccctc ttcacagaca tgcccaccgt
     2941 tgtgcagccg tacagtctcg tgctggcgtt cggcatcagt gcggggatcg ggattgtgtt
     3001 cggcctgtat ccggcgatgc gggcggcggc cctggatccg atcgaggcgt tgcggcgcga
     3061 ttgagaaaca tggcggtatg gacggggcca tgcccttccg tgcgcacgac ggcgttttac
     3121 acaaaacgcg ccctccccgc gcatctctcg tcatcattcg attccctttc gggtaacgct
     3181 ttgccgtgcc gggagggtct gctatactgt accgtgtatc gggttgaaaa agggggacgc
     3241 acatgtcaga taatcgggac acatcgaaaa cgggacgcgg atgcgggttc ggctgcgcgt
     3301 tggcgatcgg gatgttcatt gggttcgggc tgctggtcgc tgtgctggtg acaggaagtt
     3361 ttcacgtgct tggcgtggca ggggaacacg gggccatgga gtggttccgc tcgttccgca
     3421 cgcgtccgga agcgggtgtg gatgaggcgc cgaacctgat cgagacttgg tctgcgggcg
     3481 acggcgaggt taaggtcgtg cgtatcccgc ttaacgggat gatcatgctg ggggatgcct
     3541 cgtggtatgc ggggaatgcg gctgcggctc tgcgcgccat tcggaaggcc aggcatgacc
     3601 cggaggtcgc ggggctcatc ctggagatca acagcggcgg cggcggcatc acggatagcg
     3661 atatcctgta tcaggcgctg tgtgatttta aaacatcgtc caaaggccgt gtggtggtct
     3721 cgctgctcgg ggatgtggcc gcttcaggcg gctattacgt tgcgctggcg tcggatgtga
     3781 ttctggccca cccgaccacg cttaccggtt cgatcggcgt tctgctgcaa gcgtacaaca
     3841 ttcaggaact cgccgcaaag atcgggatcc gggacgttac cgtgaaatcc ggggaaaaca
     3901 aggatctctt taatccgttt caggaagtga agtccacaca gcgcgagatg gtgcagaaga
     3961 tcatcaccgc gatgcacgaa cggtttgtgg ggttggtggc gaaaaaccga acgctgccgc
     4021 tcgaaacggt ccggacgctg gcggacggac gcatctttct ggctgacgag gcgctgcggc
     4081 ataaactgat cgacgggatc gggtatcagg cggacgcgca aagccggatc gcccaactgt
     4141 tggagacgga tgcggtcaag gtcttccggt atgaagagca ggtcacgctg atggatctgt
     4201 ttacgcgtcg ggggatcggt ctgcagaccg atctggggcg ttttctgcgg gatgacgcgc
     4261 gggacgcccg tctcctgtac cgatggtcat ggtaaggtgg aaccgcgcgt gaacgtgaaa
     4321 accggagagt ggcgggtgcc cggtcgtgtc catctgctgg ggatcggcgg agtcggtatg
     4381 gcggggctcg cctatctgct ggcgcggcag ggttggcgcg tgagcggttg cgatgcgcgg
     4441 ccgaatgcgc tttgccggtg gctggaagag gcgggcattc cggtggccgg aaagcacgcc
     4501 ccggagcatc tggacgaggc ggaacgcctg atcgtcacgc cggcggtcgc gccggacgag
     4561 ccggaatggg tgcaggctcg tgcccggggg atgccggtct tctttcgcgg ggaggtgctg
     4621 gcggcatggg tttcatcgcg gcgcggtgtt gcggtttgcg gggcgcacgg caaaaccacg
     4681 accgcatgtt ttgcgacgcg gctgctcggg atgttggggc cttcacccgc ctggtgtgtc
     4741 gggggcgcga cgcgcgccat ggggaaggtc gcgtgggagg gtgcggaggg tcaggaggtg
     4801 ttcgtcgccg aagcggatga gagtgacggt acgctggcac tgtaccggcc cgcggtgacg
     4861 gtggtcacca acatggatct tgaccatctc gaccattttg cgaacgaaga ggcgctgctc
     4921 aattgttttc gtgccgtggc ggcgcagacc cgggagggtg tggcgttctg tcgcgacgat
     4981 ccgcgcgcta cactggcggc gcaggccgcg accgttccgg tattgggttt cggtttcggg
     5041 gaggctgccg atttgcgggc ctgtgcggtg gaggctgggg cggagagcgt cgcgttcacc
     5101 ccggtttacc gtggccgcga acaggcgcgg gtggtgttgt gcgtcaccgg gcgccacaat
     5161 gtcctgaatg cgttgggcgc tgctgccgca gcgctgattt tggggtgcga tccggctgcg
     5221 gttttcgcgg ccctgccgca ggcatgcggc gaattaccgg ggcggcgctt cgaatgcgtt
     5281 tctgacgagg gcgggatccg cgtcgtgaca gattacgcgc atcatccggt ggagttggag
     5341 gcggcggtgg cgatggcccg gcttcaggag ccgtcacggc tggtggtggt gtttcagcca
     5401 caccgctatt cgcgcacaaa agccttggcg cacgctttcc cccaggcgtt ggaaggggcc
     5461 gacgaggtga ttcttctgcc ggtctacgca gcatcggagc cgtggatcga gggcggggac
     5521 agcgccgacc tgtacgcgca tttccgtgag gccgggatgg gggacacgcg tgtcatgctt
     5581 gcgcgctcgc aagaggaggc gtggcagttt ttgcggcaga cgctgcaacc gggcggcctg
     5641 ttgctgttgg cgggcgcggg cgacgtgatt ggcatggtgg atgggatccg cgaggatcgg
     5701 caacggggat ggcccgcctg ccgtgatccg gaggggtttg agaagcgcgt gcgtgcgatc
     5761 gaaggacttc acgtgacgcc cttcggcacg ttgtcggagt ggtcgttttt caggacaggc
     5821 ggttgggcgc gctggcgggt ggaggcggct gacgaaaccg cgttgggtgc gctgctgaag
     5881 cagtgtcggc aagacgatgt gccgtggcgg tttgtaggcg cgggagcgaa cagttggttc
     5941 agcgatctgg gcgaaccggg gtgcgtgatc cgtcttgcgg cgtccgcctg ccgggagtgg
     6001 gaaacggcag gggacgagat ccgggttgga tgcaactgga agggggcggc gctgctggat
     6061 gagttggagc ggcgcggatt gacggggctc gaatttttgg acagcgttcc cggttcgctg
     6121 ggggggtggc tcgccatgaa tgcgggcgcg cacggcgggg aaatcggcga acgggtgcgt
     6181 tggattcgtt gtttgaattc ggatggcgaa atcactatac tgcaccctat ggatttcggt
     6241 ttcggctatc ggcggtgtgg cgggcttaca gggtgtatcg ccttgtcgtg cgctctgcgt
     6301 gtggaacgcg ccgatgcggg tgccgttcgg gtccggcgtg aggcgttccg cgcccgccgg
     6361 atttcgctgg cggggctccg aaccgagggg tcggtgttcc gtaacccgcc gggtgatgcg
     6421 gcgggacggt tgctggacac ggcgggatgc aaaggcatgc gtgtgggagg ggcccgggta
     6481 acggcgaggc atgccaatat cgtggcggta gaggatccgg tgaccgcttc ggatgtgctt
     6541 gctttggtta cgcggatgcg tcggcgtgtc gagcaggtct ggggcgtgtc gctgtcaccg
     6601 gaaatctgcg ggctgacgga aggggtaacg gaagagggaa tcggatgaag cggatcgcgg
     6661 tcatgatggg gggcacgtca agtgaacggg aggtttcgct ccgatcgggc gaggcggtgg
     6721 tcgccgggtt gcgcgaagcc gggtttgatg cggtgccggt ggtgttgaat gccgaccgga
     6781 tcgacacgtt gccgtccgct gtcgatgcgg tctttctggc gttgcacggc ggttatggcg
     6841 aaaacggcgg ggtgcagagt gatctggacc gtctcggtct gccttacacc ggaccgggcg
     6901 cggccgcgag tcggatcgcg atggacaaaa tcgcgaccaa gcgcgtgttg gaggcggctg
     6961 gtgtgccgac gccggcgttt gaggtgttgt cgcgcggcat ggaggatttc tcgctgccgt
     7021 tgccgctggt ggtgaaaccg ccgcgggacg ggtcgagtgt gggagtcgtc aaagtgacgt
     7081 ccgccgatgc gtggcccgcc gcgttggagc aggcgcgtgc gaaggatccg cggggcgagg
     7141 tgctggtcga ggcgtatatc gacggacgcg aatggacggt gagcgtgttg gacggtgagg
     7201 ccctgccggt tgtggagatt gaggcgcccg ggggctggta cgggttcacc gagaaataca
     7261 cctccggcat gacgcggtac acctttccgg aggcggtcga ggacgctccg ctggcggcgc
     7321 agtgccgcgt gttggccctg ctggcttttc aggcggtggg ttgccggggt gtcagccggg
     7381 tggatttccg tgtcacctct tcgggcgagg cctatgtttt ggagatcaac accattccgg
     7441 gattcacggc caccagtctg ttgcccaaag cggcggccaa ggcgggactg tcattctccg
     7501 agctctgcgc gcggttgatc gggacggcga catgcggatg agacgaggca cgaaacgtat
     7561 ggcatgggat tttttcaagc agggcggaac gcaacggaag cgcatgcagg ccaaggggcg
     7621 gcgtgtgacg ggcgtggtca cggacctgcc gcccggagtc cggttcgcga tccgggtggc
     7681 tgcgggtatg gtaatcctgc tcgtcgtcac atggggcggc tggcgcctct tcgaggccta
     7741 ttatttctgt tcgcgccggt tgtttgtcct gcacaatctg cgcgagaatg tgatgatcac
     7801 gaccggcagg accctgacgc cggatctgat ctgcgaagtg ctggggttgc gcgagggggt
     7861 caatctcttt tcgattccga tccggaagaa acgcaaggag ttgctggagc aggcgcccaa
     7921 tatccgggat atcacgatcg tgcgccggat gccggataag atccggatca acatcattga
     7981 gcgtgaaccg atcgcccggg tggggtcgaa cggccgggtt gtggatgaag agggggttgt
     8041 gttcatccgg tatgcgggta ccggcgggtt gccgatgatc aaaggggtgg acgcgtttga
     8101 gcagatcaag cccggtgacc ggctgagggg gaacgagttg gctgcggtgc ggctcgtgaa
     8161 caacgcccag cggccggaat gccgtctccg cctgctggtg gtggacacat cgaaagagga
     8221 ttatctgctg ttgaccctgt tcgaccaccg tcaggtgaag ttcgcttgga agggcatgcg
     8281 ggatgaggcg aaagacacgg tgtcacgcat gcagcgtcat ttcgaccagc ttgcgcaggc
     8341 catggaaagc gagatcggcc gcgcttgtct gatgtgggat gcgacacagc ccggacggat
     8401 tttcgcgacg ccgtccggat tgaatgaacc gtaaccgtag ggaagggatg gtatggcgat
     8461 tcctccaatt gcagcactgg agatcggtac gtcacgcacg gttttgtgcg tgggcgagtc
     8521 tgatgaccgt ggacgggtga aaatcaccgg tgtcggaacc tatccgacga ccggggtgcg
     8581 taaaggccag gtgtttgatc tggcgcaggc gcgcgtgggt gtggagctcg cggccaagca
     8641 ggcggaaaaa caggcggatg tcagcatctg gcaggtgctg ctggcagctt ccggcggcca
     8701 tattcaggcc gtggcaaacc ccggcatggt cccgatccgg tcaggcgatc gcgttgtgac
     8761 gcaggaggat gtcgaggaag tcacggaaaa cgcgcagacg atccaacttg acgcggaccg
     8821 gcaggtgttg cacacgatca accagtcctt cacggtggac gaccagcccg gcatcgtcaa
     8881 gccggtcggg ttgcgcggca aaatcctgac gctgaatgtg atggcggtgc atgggttgcg
     8941 taatcacatt gagaatgcga tcagtgtggc gaagagtgca cagcttgagg tgacggatgt
     9001 ggccttttcc ggcatctgcg ccggattggc ggtgctgact cccgaacagc gccgcagagg
     9061 agtggttctg atcgatctcg gcggcggcac caccaattat ctggcttacg gcaacggggt
     9121 ggtctcggcg cttggctgtg tggcggtcgg cggggatcac atcacaaacg acatcgcgtt
     9181 ggccttcacc attccgctca accgcgcgga ggaactcaag tgtgcggagg gatgcgccct
     9241 gatcgaaccg gaaagcggga cccgccgcat cactctgtcc gcagatatcg ggtttgagga
     9301 gcgggtgctg agttgcaagg cgttgcatac cgtgatccac gcgcgtatgg atgagacgtt
     9361 ccgcattgtg cgttccaaac tggacgaggg gggggtgctg ccgcatctcg gtgcgggagt
     9421 gattctgacc ggcggcggcg cgtatctgcg gaaagccact gatttggcgc aacggatttt
     9481 tggcctgccc tgcgggatcg gcttgccggt gaatgtcagc ggactggata acgtgcctca
     9541 ggctgcggcc ttgtccactg cggccggact ggtgctgtat ggccggatga cctatgagga
     9601 ccgcgggata ctctccccgc tcaagaatct cttcaagggg gtgtttcgcc gatgaatcag
     9661 gaaccgatct caggcatgct tctgatgggt atcggcggcg gcggttgccg tctggccgcg
     9721 atggtgcaat cgacgtacgg cgatggcata cgtgcgttgg ggatggatac cgatgcgctc
     9781 tccatccgca cggcctccga gggcgggctc acctgtctgc tgctgggagg ttcgcgtctc
     9841 acgggacatg gaacaggggg tgacgcgatc atgggacggc tggcggttca ggatgatttg
     9901 ccgaatctga ccgcgcacat acaaggcgtc cggagtgtcg tgctggtgac ctgtctgggt
     9961 tccggcaccg gcggcggcgc gactccggag gtggtcaaag ctctgcacag tcagggaatc
    10021 gccacgctct gtttcgttac cttgccgttc gcgttcgaag gcgagaaccg gcgtaaagcg
    10081 gcggagcgcg tgttgccgat gattgaggaa cacgcagatt cggtggtggt gctgccgctg
    10141 gatgatctgt ttgccgatac ccgtgagccg gtgctggcga ccgcgctgga agccgcggac
    10201 cgggtgctgg ccactgcggt cactttgctg tggcggctcg tgtccacacc gggattcatc
    10261 gcgctggatg aggaacggct gcatgcgatg gtggtgaaag gcggaaacgc ccgcttcggt
    10321 ttcgcaagcg cagcgggacc ggaccgcgcg ctgcgggccg cggaaatgtt gcgggaatgc
    10381 cgtacgctgc gtcatggcga tgcgctcagt aaggcgaatg ccctgctgtt cggtattctg
    10441 gcgggacggg atttgcgtct ggccgagatc ggtgatctga tggagcggat gcgcgcgtgc
    10501 tgcaaaaaag agtgtgttgt ggagatgggc accgttctgg atccggattt cgagggacgg
    10561 gtcgaactgg tcgcactggc attcgaaagt tggacggccg cctcgttggt cgcgcccatc
    10621 cctgacgtgc ccgctgggca cgagcctccg gtggcggatt cgttcccgat ccggcccggt
    10681 ggccgccgaa gccgttccaa aggaagtaaa ctctccttcg gcgcgaccgg gcgcggtaag
    10741 ttcgatcagg tggagcccac gttgcactcg ggtcaggatc tggatatccc cacgttcatg
    10801 cgccgcggga tcacgctgga acgatgagca tcgccgatga tcaagtgtcc agtcagttac
    10861 aaatgggctg tggccctgct ccttgctgcc gccgccgcgg tttcagggtg ccgcatggtc
    10921 cagtccgcgg ctgatgcgcc gggacaggct gtccgcgccg tcactccccc cagcgctcag
    10981 gccgaagaag gccccgaccc cgtcgagatt cagtcgttgg tcatgcgctt tggcgacgat
    11041 tttatcagtc gcttga
//
LOCUS       DAOSRB010000091        10564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6132_length_10564_cov_6.019222, whole genome shotgun sequence.
ACCESSION   DAOSRB010000091 DAOSRB010000000
VERSION     DAOSRB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6132_length_10564_cov_6.019222"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLZ74_06380"
     CDS             complement(<1..283)
                     /locus_tag="PLZ74_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97984.1"
                     /translation="MKKNVVTRRGFMRGAVIAGGAAALGGCSRVQATGKLRKSGEMLR
                     LGVIGAGGQGVTNWSQMLECGNVRVVAMCDTDATMLENAKGWYREKGLKA"
     gene            complement(391..2079)
                     /locus_tag="PLZ74_06385"
     CDS             complement(391..2079)
                     /locus_tag="PLZ74_06385"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOR97985.1"
                     /translation="MKTSLLLVLGSLSAGGSLLFAQPAPEARFAVMTPDEIRAYERDT
                     LQRLADLSLIPPIINTHPLPKYDYDQLDYGMTLGIARTPGGRLWAAWVAGEDGPKAFM
                     VAATSDDDGETWSKPRFVVDSQAPHLPIPRSVIVGNFWTDPSGRLWFFFDQTMNHYDG
                     RQGVWVTVCTNPDADQPAWSKPKRIWHGAVLNKPTILANGEWWLPIEFPAFSGIGPMR
                     HANQDLEHLRGANVFVSTDQGQTWRRRGCVRFPKPDWDEHMFVELKDGRIWMVARTRK
                     GPMQSFSSDGGKTWTEPDFPSFKHPVARFHIRRLASGRILLVKHGETIDTHEGRSKLT
                     AWLSEDEGQTWKGGLMLDERTGISYPDGFQHPDGTIYISYDRNRSTDGEILLARFTEA
                     DILGRTISSPRSKLKMLISRPLKNRLREMEFNTAAKPLRTEPMAEFKSGKGEIRPLEK
                     GAKIYTNRMYTFHDLPEPLTGRRFVYSGIDSTEAVCSRAGMVYVFTPSTKRNPHDNVV
                     HELFSQGFEKTTVPEFVYTLVDGQARSHETCSVFQKQVKVGETVRFGRWGILVF"
     gene            complement(2048..2425)
                     /locus_tag="PLZ74_06390"
     CDS             complement(2048..2425)
                     /locus_tag="PLZ74_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97986.1"
                     /translation="MSMQVRFALSLLPVFVCVSNTFSAPAFEGAFSGIAPDGSRGIYH
                     GASVGMATLRRDGFASMDGNGTLTPFGSGSASPTLACTLSGSARPSLAQAAAVLPLAA
                     PATPVTSIPKEPFHEDFPSACPR"
     gene            2651..4030
                     /locus_tag="PLZ74_06395"
     CDS             2651..4030
                     /locus_tag="PLZ74_06395"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOR97987.1"
                     /translation="MHANTLILIVERGPLVGHRFPVPPNGLLLGRSSRCDLAFLDPSL
                     SRRHCRLEWRGMVLWVVDLNSANQTLVNGQPIHESPLMPNDTITVGETTLRVRQEEVP
                     TVSLSDLPDQDIAVGRVPPPFAAEGGEMLESCALRRRSVRLSLAIGGVLTLLCGAGLL
                     YRLRMDTGAGERIALPTAEFGAATQSDSVPPAPAADPDRSVSSTEADAATTAPKTETE
                     SGRSSSRPAPDQAGDRSDPSASPVRMDDAPQGEKPLPCSLRVESDPPQASVIVNGTDR
                     GETPCVLADPKPGVYTLRVEKSGYETLTRKIMLEGGDAVTERFRLESVTGSLAIRTAP
                     SIVTVMLDGKKVGITRPRGTDASGASEPLVLRDLPEGEHQIEVRRKGYATQRRTAIVK
                     RGEHVAVALALPRQFIPNYEVRTSRSYYRGVLEFIDAEGIRLETAPGVTQTILLRDVK
                     QHGALPAEE"
     gene            4033..6531
                     /gene="hrpB"
                     /locus_tag="PLZ74_06400"
     CDS             4033..6531
                     /gene="hrpB"
                     /locus_tag="PLZ74_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOR97988.1"
                     /translation="MFPVDRILPALADSLAANRTVVLQAPPGSGKTTRVAPFLLDAGA
                     PWLAGRKILLLEPRRLAARAAAAYMARQRGEAIGDTIGYHIRLERRIGSQTRIEILTE
                     GLLTRRLLDDPELADTGLILFDEFHERSLAADMGLALALDARRALRPDLRLVVMSATI
                     RPGPVATHLGDTDIHEADARCYPVETRLSGHVSTMPLPIRVAEAVIRALHDEPGSVLV
                     FLPGEGEIRRTLERLQGAELPPGVSLYPLYGALSPAEQDAAVAPAPPGRRKVVLATSI
                     AETSLTIEGIRVVIDSGWMRVPRFSPRHGMTRLETLRVTRDRADQRRGRAGRLEPGVC
                     YRLWDEAADRQISPEALPEILEADLAATVLQAADWGSAAPDGLPWLTPPPDAGWRQAV
                     GLLHELGALDGPAPDPAAADRVWPAITPRGREMTRRPIHPRLAHMILEAARKGCARQA
                     CLVAAVLSEAPAESALRREIDVRVLLDRLEYAGGSRNRFAFRVRELARQWGHAYPARD
                     TRELPVGRLLAWAYPERIARRRDAAGRYLLRSGRGAVLEPTESLAQAEWLVAAELQDG
                     AAEARIRLAAPLTVEEVEEAFDGEFEDRAETYWDARTEAVQAVRRIRLGAITLREGGQ
                     AAPDPAALRAALYEGIRQKGVDQLPWTRSARALQARVVFLRQAMPEGGWPDYSDEALA
                     ADLDAWLGPHVEGIMRWNRVQALDLTSILWGALNAHGVSRRRLDEFAPTHIRVPSGSS
                     IPIRYEGDTPYLHVRIQEVFGMTQTPRVAAGRVPVVMSLLSPAQRPVQVTRDLESFWN
                     TGYALVRKDLRGRYPKHYWPEDPREATATRRVRP"
     gene            6751..9351
                     /locus_tag="PLZ74_06405"
     CDS             6751..9351
                     /locus_tag="PLZ74_06405"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97989.1"
                     /translation="MKGKGIWCWVWGIVGVVWAGTAKLPDLPPQDLPPPAVTPDWLVA
                     MASRHGEDAAREYRLLKREMTTKPMTLSGTVDAAARLAQTFHQQAAILPTDRDPLDVV
                     LRRTRALYEDLAPQVELTAAGARLAELEAEARGVAAVDVPARADCFGRVVALRKTIAF
                     ANPLLKGIDRILFITREALPPNEFDWGVHMCDQYYGFHATVHGTTQGNGLYVLHAPWS
                     ERPRAQNLLAESVIESGARKGQKLGNGGYLSPDLSYDGRQILFAYTDGEPQIRVWNEK
                     TTFHLFRCNADGSGLVQLTDGNVNDFDPCWLPNGRIAFISERRGGYGRCHGRLVPTYT
                     LHSMFEDGSDIVAISVHETNEWQPSVDNNGMIVYTRWDYVDRGFNQAHHPWITYPDGR
                     DARELQGNFHLSERTAPHMVMDLRAIPGSSKYVAIACGHHTEARGSVIILDPTLPDDG
                     AMSQIRRFTPDQLMPEAEYYDWKTTKGSAAYASPWPLSERYMLCVYDGDANAQYGVID
                     YKKRRYAITLLDAFGNKIPLYTHPQISCLSPIPLVPRPRPPVLTHGTQTGRPRLPNGA
                     KPAPIPEAERTSKATVGLINVYNSRYPFPAGTVITALRVWQILPKTEPIVGKPRLGVC
                     DQTPGRQFLGTVPVEKDGSAFFEVPAGVPILFHAVNEKGAAVQGMRSLTYTAPGETLM
                     CAGCHESRTGAAARDTVSSKPVAMRRAPSVLRPGPDGSNPFNYPRLVQPVLDARCVTC
                     HGADRKEGMPDLRKGEVAKNRYRFHTSFVSLVPYVCYYTLMYHGPGWLTMGVQRDSFV
                     EPFTLPGRFGSYASRLHAMLAKGHHNVKLTEEENLRLTLFMDSNAAYFGHDHELERQA
                     LGEIVHPVLQ"
     gene            9425..10393
                     /locus_tag="PLZ74_06410"
     CDS             9425..10393
                     /locus_tag="PLZ74_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97990.1"
                     /translation="MKRLARMGLLKLCAAAVASAGAQTLGTGTGGLILSDDNRTVLAP
                     PPVHADGELIMAELPLRVMPPRVSFLFGTSGRHGPIDLTDGSRVGNPRAPYVLRLVEG
                     GASFTLSPESATNQVSGPFSTADRTVFTLSGAKVTLVRMPPELRVAVRHAGETGQAPK
                     IGVAPYTPEVTKALYALRTRYVGLANRVDVETADIAFQGVPRVHSRITGNTFTPVVKR
                     SERDKQQTLKGAERSAVTFLDSLFRQAFSLRSQAITDGCVYHFRMPPGEYLLCVMQPV
                     RDPDARGNMGSATAIWWTAFYFDGERSLEVELNGENAITWRDLFKL"
     gene            10484..10560
                     /locus_tag="PLZ74_06415"
     tRNA            10484..10560
                     /locus_tag="PLZ74_06415"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10518..10520,aa:His,seq:gtg)
BASE COUNT         1911 a         3157 c         3487 g         2009 t
ORIGIN      
        1 ccgccttgag ccccttttcg cggtaccagc ctttggcgtt ttccaacatc gtcgcatcgg
       61 tgtcgcacat cgccaccaca cgcacgttac cgcactctaa catctgcgac cagttggtca
      121 cgccctgtcc gccagccccg atcacgccca gacgcagcat ctcgccgctt ttacgcaact
      181 tgccggttgc ctgcaccctg ctacatccgc ccaaggccgc cgcacccccg gctataaccg
      241 cccctcgcat aaacccgcgc cgcgttacaa cattcttctt catcgttccg aacctcctct
      301 tggcttgctc gacgtaaact gattttttaa tccgtttcaa agtacgccaa accgcttggt
      361 ttttcaagat gacagttgcg catatttaag ttagaaaaca aggatacccc agcggccaaa
      421 ccggacggtc tcgccaactt tgacctgttt ctggaagacc gagcaggtct catgggaacg
      481 cgcctgcccg tcaaccagcg tatagacaaa ctcaggaaca gtggttttct cgaacccttg
      541 tgagaacagc tcatggacga cattgtcgtg cggattgcgt ttggtggacg gcgtgaacac
      601 atagaccatt cctgcccggg aacagacggc ttcagtactg tcgattcccg aataaacaaa
      661 acgcctgcct gtcagcggtt cggggagatc gtggaaggtg tacatgcgat tcgtgtagat
      721 cttcgcgccc ttttcaagcg ggcggatttc gcccttgccg gatttgaatt ccgccatcgg
      781 ctctgtccgc aaaggcttgg ccgcagtatt gaactccatt tcgcggagac ggttcttgag
      841 cggtcggctg atcagcattt tcagcttcga gcggggcgag gagatcgtcc gaccgaggat
      901 atccgcttcg gtaaaccgag cgagcaggat ttctccgtcc gtggagcggt tgcggtcata
      961 ggagatgtag attgtgccgt ccggatgctg gaagccgtcc ggataggaga tgccggtgcg
     1021 ttcgtcgagc atcagcccgc ccttccacgt ctgcccctcg tcctcagaaa gccaagccgt
     1081 cagtttggag cgcccttcgt gggtatcgat ggtctctcca tgtttgacaa gcagaatgcg
     1141 tccggatgcg aggcggcgga tgtggaaacg cgcgacagga tgtttgaaac ttgggaaatc
     1201 aggctctgtc cacgttttac ccccatcgga ggaaaaactc tgcatcggtc ctttacgggt
     1261 acgggcgacc atccagatac ggccatcctt gagttccacg aacatgtgct catcccagtc
     1321 aggtttagga aagcgcacac aaccgcggcg gcgccacgtc tgcccctggt cggttgagac
     1381 aaagacgttc gcgccgcgca ggtgctccag atcttgattg gcatgacgca tcggtccgat
     1441 gccgctgaaa gccggaaatt caatggggag ccaccattcg ccgttagcca agatcgtggg
     1501 cttattgagc actgcgccat gccaaatccg tttcggtttt gaccaggcgg gctggtcagc
     1561 gtcgggattc gtgcagaccg tgacccagac gccctggcgg ccgtcgtagt ggttcatggt
     1621 ctggtcaaag aagaaccaga gacggccgga cggatcagtc cagaagttgc cgacgatcac
     1681 gctgcggggg atcggcaggt gtggtgcctg actgtccacg acgaagcgcg gtttggacca
     1741 ggtttcgccg tcgtcgtcgc tggttgcggc gaccataaaa gccttggggc cgtcttcgcc
     1801 agcgacccaa gcggcccaga gacgtccacc gggggtgcgg gcgatgccga gcgtcatacc
     1861 gtaatcgagt tggtcgtagt cgtacttcgg cagcggatgg gtgttgatga tgggggggat
     1921 gagcgagagg tcggctaggc gctgaagcgt atcgcgctca taggcgcgga tttcgtcggg
     1981 cgtcataacc gcaaaacgcg cctcgggggc gggttgcgcg aacaggaggg atcctcctgc
     2041 gctaaggcta ccgaggacaa gcagaaggga agtcttcatg gaagggctcc ttgggtatcg
     2101 atgtgaccgg tgtagccggg gccgccagcg gcaagacagc cgccgcttgc gccagactcg
     2161 gacgggctga cccagaaagc gtacaggcta gcgttggtga ggcggaaccg gaaccgaacg
     2221 gggtgagggt tccgttgccg tccatggagg cgaacccgtc gcggcgcagg gtagccatgc
     2281 cgacgctcgc cccgtgatag atgccgcgcg aaccatccgg cgcgatgccg gaaaacgcgc
     2341 cttcgaacgc aggggctgaa aaagtgttcg agacgcatac gaaaacaggc aacagcgaca
     2401 acgcgaaacg gacttgcatg gacatatttt catttaacag caacgactgc ctgttgtcaa
     2461 tcgcacgtca cactgctcgc catgcgtact ttcacggatc agcctcgtgt tctacatgtt
     2521 ccccctgcct gttttaaagt gcgtcaaaaa cggaatggct cgttcaggcg tgacagacac
     2581 ctgtaaaacc ggataatatg aatctgtcgg acaggacgtg atgtggcgac aagggaaacg
     2641 gatacggaag atgcatgcga acaccctgat actgatcgtg gagagagggc cattggtggg
     2701 ccaccggttc cccgtgccgc cgaacggcct gctgttgggc cggtcgagtc ggtgtgatct
     2761 cgcgtttctc gatccctcgc tttcccgccg ccactgccgg ctcgaatggc gtggcatggt
     2821 gttgtgggtg gtggatctga acagtgccaa ccagacgctg gtcaacggtc agccgatcca
     2881 cgagtcgccc ctgatgccga acgatacgat cacggtcggc gaaacgacgc tccgcgtccg
     2941 gcaggaagag gtgcccaccg tgtcgttgtc cgacctgccg gatcaggata ttgcagtggg
     3001 cagggtgccg cccccgtttg cggcggaagg cggggaaatg cttgaatcgt gtgccctgcg
     3061 gcggcgttcc gtgcggcttt ccttggcgat agggggtgtg ctgacgctcc tttgcggcgc
     3121 ggggctgctg taccggttgc gcatggacac cggcgcgggc gagaggatcg cgctgccgac
     3181 cgcagagttc ggcgcggcaa cgcaatccga ctctgtacct cccgcaccgg ccgcggatcc
     3241 ggatcgatcc gtttcgtcta ccgaagccga tgcggcgaca accgcgccga aaacggaaac
     3301 agagtccgga cggtcttctt ccagaccggc accggatcaa gcgggggacc gttcggatcc
     3361 ctccgcctcg ccagtgcgga tggatgacgc gccgcagggc gagaagcctt tgccgtgttc
     3421 tctgcgcgtg gagtccgatc cccctcaggc gagcgtcatc gtcaacggta ccgatcgggg
     3481 tgaaactccc tgcgtgctgg ctgatccgaa acccggggtg tacacgttac gcgtggaaaa
     3541 aagcgggtat gagacgctga cccgcaaaat catgttggag ggcggggacg cggtcacaga
     3601 gcgtttcagg ctggaatccg tcaccggcag tcttgcgatc cgcaccgcgc cctcgatcgt
     3661 gaccgttatg cttgatggta aaaaagtcgg catcacccgc ccgcgcggta cggatgcgtc
     3721 cggtgcgtcg gagccgttgg tgctgcggga tctgcctgaa ggtgagcatc agattgaggt
     3781 tcgccgcaag gggtacgcga cccagcgccg gacggcgatc gtgaagcgcg gagaacacgt
     3841 cgccgtggcg ctcgctttgc cccggcagtt catacccaat tatgaggtcc gcacctcacg
     3901 ctcgtactac cggggcgtgc tggagttcat cgacgccgag gggatccgct tggagacggc
     3961 gccgggtgtc acccagacca ttttgctgcg ggatgtcaaa cagcacggcg cactgccagc
     4021 ggaggaatag gggtgtttcc ggttgaccgc attcttcccg ctttggctga ctcgctggcc
     4081 gcgaaccgta cggtagtgct tcaggcgcct cccggatccg gcaagacgac gcgcgtcgcc
     4141 ccctttctgt tggacgcagg tgccccttgg cttgcgggcc gcaaaatcct gctgctcgag
     4201 ccgcgccgtc tggcagcccg ggctgcggcg gcatacatgg cccgccaacg cggcgaggcg
     4261 atcggcgaca cgatcggtta ccatatccgc ctggaacgca ggatcgggtc gcagacccgt
     4321 attgagatcc tgaccgaagg gttgctgacc cggcgtctgc tggatgaccc ggaactcgcc
     4381 gacacgggac tgatcctttt cgatgagttt catgaacgga gtctggcggc ggacatgggg
     4441 ctcgccctcg cgctcgacgc gcgccgtgca ctgcgtcccg atctgcggtt ggtggtgatg
     4501 tccgccacga tccgtccggg tccggtggca acacatctcg gtgacacgga catccatgag
     4561 gcggacgcgc gttgttatcc ggtggaaacc cggctgtcgg gccatgtgtc cacaatgccg
     4621 ttgccgatcc gggtggcgga agcggtgatc cgggcattgc atgacgagcc aggcagcgtt
     4681 ctggtctttc tgcccggcga gggcgagatc cgccgcaccc ttgaacgttt acagggggcg
     4741 gaactgccgc ccggggtctc cctgtacccg ttgtacggcg cgttgtcgcc tgcagagcag
     4801 gatgcggcag tcgcaccggc gccgcccggt cggcgcaagg tggtgctggc cacctcgatc
     4861 gcggagacct cgctgaccat tgaagggatc cgtgtggtga tcgattcggg atggatgcgc
     4921 gtgccgcggt tttcgccccg tcacggcatg acccggctcg agaccttgcg cgtcacccgc
     4981 gaccgcgccg accagaggcg cggtcgcgcg ggtcgtctgg agcccggagt ctgttaccgg
     5041 ctgtgggatg aggcggccga ccgccagatc tcccctgaag ccctgccgga aatcctggag
     5101 gctgatctcg ccgccacagt gctgcaggcc gcagattggg gttccgccgc gccggacggg
     5161 ctgccgtggc tcacgccgcc gccggatgcg ggctggcgtc aggctgtggg gttgttgcat
     5221 gagttgggtg cgctggacgg tcctgcgccg gacccggccg cggccgaccg cgtctggccc
     5281 gctatcacgc cccgcggacg cgagatgacg cgacggccaa tccatccccg tcttgcccat
     5341 atgattctgg aagcggcccg gaaagggtgt gcccgtcagg cctgcctcgt tgcggccgtc
     5401 ctctccgagg cgcccgcaga atccgcactg cgtcgggaga ttgatgtgcg tgtgctgttg
     5461 gaccgtctcg agtatgcggg cggaagtcgg aaccgctttg ccttccgggt gcgcgaactg
     5521 gcccgccagt ggggccacgc ttatcccgcg cgggatacgc gggagcttcc agtggggaga
     5581 ttgctcgcct gggcgtatcc tgagcgtatc gcgcgtcgcc gcgatgcggc gggccgctat
     5641 ctgttgcgta gcggacgcgg cgcggttctg gaaccgaccg aatcgttggc gcaggccgaa
     5701 tggctggtgg ctgcggaact tcaggatggc gctgcagagg ctcggatccg tcttgccgcg
     5761 ccgctcacgg ttgaggaggt ggaggaggcg ttcgacggtg aatttgagga tcgggccgag
     5821 acgtattggg atgcgcgcac tgaggccgtt caggcggtcc gcaggatcag gctgggcgcc
     5881 atcacgctgc gcgagggtgg ccaggccgcg ccggatccgg ccgcgctacg tgcggcactg
     5941 tacgaaggga tccggcagaa aggggtggat cagttaccgt ggacccggag tgcccgtgcc
     6001 ttgcaggccc gcgtggtgtt tctgcgtcag gccatgccgg agggcggctg gcccgattat
     6061 tccgatgaag cgctggcagc ggatctggat gcgtggctgg ggccccatgt cgaggggatc
     6121 atgcggtgga accgggtaca ggcactcgat ctgacgtcga tcctttgggg agcccttaac
     6181 gcgcacggcg tgtcgcgacg gcggctggac gaattcgcgc ccacacacat ccgggttccg
     6241 agcggctcgt ccatcccgat ccgctatgaa ggggacacgc cttatctgca tgtccgaatc
     6301 caagaggtgt tcggcatgac gcaaaccccg cgggtggcgg caggccgtgt tccggtggtg
     6361 atgagcctgc tctcaccggc ccagcggccc gtgcaggtca cgcgcgatct cgaaagtttt
     6421 tggaacaccg gatacgcgct ggtgcgtaaa gatctccggg ggcgttaccc aaaacactac
     6481 tggccggaag atccccgcga ggcaacggcc acacgccgcg tacgtccctg acgccgttcc
     6541 gcgcacagat gacggatgca gccgaccgga tttttgtcat gccgggaggt cgacggatca
     6601 gcgggagttt ggttcaatgg tggtgatggc tgggtggatc cgtgacagga accacacaat
     6661 cttcgcaatg acggaggcgg atggcctcgt tgatgaccgc agcgatctgt tcggcgaaga
     6721 gaatggatat cgggaacggg ggcaagaaaa atgaagggaa aagggatatg gtgctgggtg
     6781 tgggggatcg tgggagtcgt atgggctggg acggcgaaac tgccggacct tccgccgcag
     6841 gatcttccgc cgccagccgt cacgccggac tggctcgtgg cgatggcgtc gcgccatggt
     6901 gaggatgcgg cccgggagta ccggctgctg aaacgcgaga tgacgacaaa gccgatgacg
     6961 ctctccggca cggtcgatgc tgccgcgcgt cttgcccaga cgttccacca acaggcggcg
     7021 atcctgccga ctgaccgtga cccgctggat gtggtgctgc gccgcacacg cgccctgtat
     7081 gaggatctgg cgccgcaggt ggagttgacc gcagcgggcg cgcggctggc ggagttggag
     7141 gccgaggccc ggggggtggc cgcggtcgac gtcccggcgc gcgcggactg tttcggtcgg
     7201 gtggtcgcct tgcgcaaaac catcgctttc gccaatccgc tgttgaaggg tatcgaccgc
     7261 attctcttca tcacgcgcga ggcgctgccg ccgaatgagt tcgactgggg tgttcacatg
     7321 tgcgaccagt attatggttt ccacgccaca gtgcacggca cgacgcaggg caacgggctc
     7381 tatgtcctgc acgcgccgtg gtcggagcgg ccgcgggcgc aaaacctgct cgccgaatcg
     7441 gtgatcgagt caggcgcgcg caaggggcag aagttgggta acggcggata tctttctccg
     7501 gacctctcct atgacgggcg gcaaattctg ttcgcgtaca cggacggcga accgcagatc
     7561 cgcgtgtgga acgaaaagac gaccttccat cttttccgct gcaacgcgga cggttcgggg
     7621 ctggtccagc tcaccgatgg aaacgtgaat gatttcgatc cctgctggct gcccaacggc
     7681 cgcatcgcct ttatctccga acggcgcggc gggtacgggc ggtgtcacgg tcgcctggtg
     7741 ccgacctaca cgctgcacag catgtttgag gacggtagcg acatcgtggc gatcagtgtg
     7801 cacgagacca acgaatggca gccgagcgtc gataacaacg ggatgatcgt ctacacgcga
     7861 tgggactatg tcgaccgcgg cttcaatcag gcgcaccatc cgtggatcac ctatccggac
     7921 ggacgcgacg cgcgcgagtt gcaggggaac ttccatcttt cggagcgtac cgcgccgcac
     7981 atggtgatgg acctgcgcgc catccccggt tcctcaaaat acgttgccat cgcctgcgga
     8041 caccacaccg aggcgcgcgg ctcggtgatc attttagacc cgacgttgcc ggatgacggg
     8101 gccatgtcac agatccggcg cttcacaccg gaccaactca tgcccgaggc cgaatattac
     8161 gattggaaaa caaccaaggg gtctgcggcg tacgcgtcgc cttggccgtt gagcgagcgc
     8221 tacatgctct gtgtgtacga cggggacgcc aacgcgcagt acggcgtgat tgactacaag
     8281 aagcggcgtt acgcgatcac gctgctggat gcgttcggaa acaagatccc gctctacacg
     8341 catccgcaga tctcctgcct cagcccgatc ccgctcgtgc cgcggccccg gccgcccgtg
     8401 ttgacgcacg gtacgcaaac gggtcgcccg cgcctgccaa acggcgcgaa gcccgcgccg
     8461 attccggaag cggaacggac atcgaaggcc accgtcggac tgatcaacgt ctacaactcg
     8521 cggtacccgt tcccagcggg cacggtgatc actgctctac gggtctggca gattctgccc
     8581 aagaccgaac cgatcgtggg caagccccgc ctgggggtct gcgaccagac gcccggacgt
     8641 cagttcctgg gcacggtgcc ggtcgaaaag gacggcagcg cgttcttcga ggtgccggcc
     8701 ggagtcccga tcctcttcca tgcggtgaat gagaagggcg cggcggttca gggcatgcgc
     8761 agtctcacgt acacggcgcc gggcgagacc ctgatgtgcg ccggttgtca tgagtcgcgc
     8821 accggcgcgg cggcgcgtga cacggtgtcc tccaaaccgg tcgccatgcg gcgcgcgccg
     8881 tccgtgttgc gtcccggacc cgacgggtcg aaccccttca attatccgcg tctggtgcaa
     8941 cccgtgctcg atgcccgttg cgtcacgtgc cacggggcgg atcgcaagga ggggatgccg
     9001 gatttacgaa agggagaggt cgcgaaaaac cggtaccggt tccacacctc attcgtcagt
     9061 ttggtgccgt atgtctgtta ttacacccta atgtaccacg ggccggggtg gctcaccatg
     9121 ggtgtgcaac gcgactcctt tgtcgaaccc ttcacattgc ccggaaggtt cggttcgtac
     9181 gcgtcgcggc tccatgcgat gctggccaaa ggtcatcata acgtcaagct cacggaggag
     9241 gagaatctcc ggctaacgct tttcatggac tccaacgcgg cgtatttcgg tcatgatcac
     9301 gagttagaac ggcaagctct tggcgagatc gtccatccgg tgctgcagtg agtggacagg
     9361 ccaccggcgt tccgggatgg ggtgtcaagg caaggtgaag cggtaaaatc tgaaaggcgg
     9421 ggctatgaag cgtcttgccc ggatggggct cctgaaactc tgcgcggcgg cggtggcaag
     9481 cgctggggcg cagacgttgg gcacaggcac cggcgggctt attttgtcgg atgacaaccg
     9541 gacagtgctg gcgccgcctc cagtgcatgc ggacggggaa ctgatcatgg cggaactgcc
     9601 gctgcgggtt atgcccccgc gcgtctcttt cctgttcggc acgagcggcc gccacggacc
     9661 gattgacttg acggacggga gccgggtcgg gaatcccagg gcaccctatg ttctccgttt
     9721 ggttgagggg ggcgcgtcct tcacgctgag tccggaaagc gcgacgaatc aggtgtccgg
     9781 tcccttttcc acggcggacc ggacggtctt caccttgtcc ggcgcgaagg tgaccctggt
     9841 ccgcatgcct cccgagctgc gcgtggctgt gcggcatgcc ggagagaccg ggcaggcacc
     9901 aaaaatcggc gtggcaccgt atacccctga ggtgacaaaa gccctgtacg cattacgaac
     9961 ccggtacgtc ggactggcga accgcgtgga tgttgaaaca gcggatatcg cgtttcaggg
    10021 tgtgccgcgt gtccactccc gaatcaccgg caacaccttc acgccggtgg tcaaacgctc
    10081 ggaacgtgac aaacaacaga cgctcaaagg cgcggagcgg agtgcggtca cgtttctgga
    10141 ttccctcttc cgccaagcct tttcccttcg gtcgcaggcg atcacagatg gatgcgtcta
    10201 ccatttccgt atgccgccgg gagaatacct gctgtgcgtg atgcagccgg tacgcgatcc
    10261 ggacgcgcgc gggaacatgg gttcggccac cgcgatctgg tggaccgcgt tttattttga
    10321 cggtgaaaga tcgctcgagg tggagctgaa cggggagaac gcgatcacgt ggcgtgacct
    10381 gttcaagctg tgagatgcgg attttcccgt ttgacgaagg aaggcgtacg aattgcgggc
    10441 ttgccttttc aggacgggtt tggtaaaaga ccctcacttt ctggcgaaaa tagctcagtt
    10501 ggttagagcg gcagattgtg gttctgcggg tcgcgggttc gagtcccgtt tttcgcccca
    10561 tttt
//
LOCUS       DAOSRB010000092        10551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6145_length_10551_cov_6.780678, whole genome shotgun sequence.
ACCESSION   DAOSRB010000092 DAOSRB010000000
VERSION     DAOSRB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6145_length_10551_cov_6.780678"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..751
                     /locus_tag="PLZ74_06420"
     CDS             392..751
                     /locus_tag="PLZ74_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97991.1"
                     /translation="MRKEKENDIELGKVLTKGMTFTYEYDFGSTTELVLKVVGASQRH
                     DVPLDVPIVLARNDPPAWVCADCGKPVVRVDAMTGYGACKDTVFCAACAKKRRKTGEC
                     YCLKLVNSPRTGVCAYE"
     gene            complement(858..2279)
                     /locus_tag="PLZ74_06425"
     CDS             complement(858..2279)
                     /locus_tag="PLZ74_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR97992.1"
                     /translation="MQTRREFIGGAAAVFSLAGCQSTGRFAARPDLKFGVISDTHVTT
                     PESTARLREALRYFRRRGADAVMIAGDLTDWGLKSGYDYLAEAWKDEMAGTGIELLMC
                     TGNHDYDGWWYGDMTLDMHMQGYSEDDALCDLGMGECWEKSFGEQFGEIRRRTIKGYH
                     FISAEFNWKNKTCGDEMIAKWLSDHADELKTPGRPFFFFRHIPIPGVAAGASKDDKPN
                     PLKTTLDGFANGFVFTGHTHRTLNDEKSIWQDGFTAISVPSLSYTSAPRGYDNGSDSR
                     KGDSTLGMRRIPSRENLEQPQGFFVSVYPTRVEIERHDFGERTDIADPWIVPEGKKPY
                     TFEAQRNRVPVPQFPSGATVRTFVSNGDTRNGRWTIFMTLEFPAAKATKGRAFDYEVR
                     AEMTDGTVAAVKRFLSPTFHRAHWRDADVLRFDFDGMSLPDNGDYRLKVYPRNSLGVA
                     GRPIASCLFSPKPGKATVQKKRG"
     gene            complement(2294..4150)
                     /gene="mgtE"
                     /locus_tag="PLZ74_06430"
     CDS             complement(2294..4150)
                     /gene="mgtE"
                     /locus_tag="PLZ74_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOR97993.1"
                     /translation="MIPILKAIAEGRLIELPPCDKNQALRTLAEKLCASADIPAEYNV
                     FENVIRREKQAITYLKYGIACPHARSEEHGGEMACVIGWSPDGIDYGNTDGWPVHLIL
                     MYYIPGSARNAYLTELATLARAIEDDETKHELVNLKDLEDVQSRLESWIATIEGREDA
                     EDDREQVRRATSTVLSQLLMPDIIEMLEDRRFNDLRIFLAAQPTPEIAELIATLQTSD
                     QLLVFRLLPRSMAGEVFSLLEYPSQNLLLENMAQDETRHVLTALSPDDRTALFEELPA
                     NVLQGLLNLLSDKDRRQALSLLSYPKDSVGRLMTNRYVFAREEWTVARTLEQIRAMGN
                     DSETVMMIYIIDERGVLVDELVLRKLILADPDTPISSLMDHQYVALHSLQDREEAVMV
                     FKKYDLHVLPVVDSEGVLLGIVTNDDILDVSEEEATEDIHKGVAIMPLSAGYLRTSLS
                     ILYRSRLPWLVTLVFVNIFSGAGIAHFDTLIGSFVALVFFLPLLIASGGNAGAQSATL
                     VIRGMALGELTLKDFMKVLWREIVVSLSLGISMSAAVFVLAWWRGGLRIGVVAALSMT
                     LIVVSGSLIGMTLPFILRKLKIDPAVASSPLVTSLADILGVFIYLGIASALL"
     gene            complement(4249..6579)
                     /gene="ligA"
                     /locus_tag="PLZ74_06435"
     CDS             complement(4249..6579)
                     /gene="ligA"
                     /locus_tag="PLZ74_06435"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOR97994.1"
                     /translation="MNRERLEHLRAEIRRHDHLYYVEARPEIGDADYDALYRELEALE
                     RAHPEWVTPDSPTQRVGGAPLDAFRQVRHDPPMMSLDKTHSRGDLTDFDAFLRRQMPD
                     EVWDYVVEPKIDGVAFSLVYARGALTRAATRGNGEVGDDITANIRTLRSIPLVLRDAP
                     SLLEVRGEVYMTREGFAALNRRMEEAGREPFMNPRNAAAGSLKQLDPREVARRPLDAV
                     LYAAVDGSGVEFPTHGGLLARLAEWGFRTPPWQRLCVDIQAVLDAIDELQSLRHAFPF
                     EIDGAVIKVNRRDLYARLGSTAKAPRWARAFKYEPERATTRIGSVTVQVGRTGVLTPV
                     AELTPVLLAGSEIARATLHNADEIARKDIRIGDRVWIVKAGDVIPAVESVQTEERDGT
                     ERVFVMPSVCPECGGAVVRLEGEVAHRCTNPACPAQRVGRLEHFASRDALDIRGIGGK
                     VAEALVAQEWVRDPLDLFDLTLPQLEAFRIGDEQSGTRKFGKNAQTALQSLEAARTLP
                     LDRWLFATGIPSIGVTVAEQIASVHKNFSSLAESPILQAVLRLDELYAQATTVNPRST
                     ANRPKDEAEREERQNLFNRICGEIGVTGDALVAADAATRTPGSTLPPYYTCKIKPEAA
                     KAVLAFFASEYGARYLQRLKDLGIDPCARPKPAAVEGPLAGLTFVLTGTLSQPRRTLA
                     ARIRTAGGTVQDTVTRQTRYLVAGTDAGGVKCDKARALGTEILDEAGLESLLGGTPQN
                     TPPTPPPSATPTPPPGAQAAYRQTELFPSRSVDFRG"
     gene            complement(6599..7585)
                     /locus_tag="PLZ74_06440"
     CDS             complement(6599..7585)
                     /locus_tag="PLZ74_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOR97995.1"
                     /translation="MNARKRIQPPPRNSPRIGIALGSGGARGWAHLGVLRRLDAFGVR
                     PVCVAGTSIGAIVAALYACNALDTTEALAARLDWKQLAQLFFEVTFPRSGLITGKNFM
                     RLLKDIIPARNIADLPFPYAAVATNLHTEKEVVLSSGNLFDAIRASIGIPGIFTPTRL
                     NGQTLVDGGLVNPLPVSVCRALGAEIVIAVDVNLRTPPLRPRRTPRKPADAPNPHVDA
                     LIASISRLMPDALQTPMSELAHRWTAPRKHNADPLSIFDVLTHTMRLVENQITRHELA
                     LHPPDILIQPEVGNLLTLEFHRGLEGIAAGVRAADEQRSHIEALLNPRPAPS"
     gene            complement(7582..7938)
                     /locus_tag="PLZ74_06445"
     CDS             complement(7582..7938)
                     /locus_tag="PLZ74_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97996.1"
                     /translation="MTIRFFAAVLSVTLIMLGGSGCTTSRVIENTRVPEITIEESGTI
                     RFNQEPVEPGKLVSALRSGGIRTSQEVNILIPDQPDRALMRAVSAELLRGGYSRTVFV
                     KNRKATSSFPSARKTK"
     gene            complement(8037..9101)
                     /locus_tag="PLZ74_06450"
     CDS             complement(8037..9101)
                     /locus_tag="PLZ74_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97997.1"
                     /translation="MRVWLPIVMCFLLTVAAVWQSRRLAVQRRESMPETYAGSAERPP
                     PALTFLLVGLGGFRGIAAEALWFRINRLQEEGRFLELVQLSDWITRLDPHAADAWIYN
                     AWNLAYNVSILMIREEDRLRWVRNGLTLLRDDALRFNPREARLYRELAWLYQNKIGDS
                     LDSAHLTYKRHLATLLSPHVNPDGTVRITEESRSALAAMRLDADRMRALENRFGPLDW
                     RLAESHAVYWATQGLDLATGTERLLCRRAVYQPLLLSVWRGRLTGDPEGPEWQTAPNP
                     ALASATAAFLIETLSEHPTATQRNICVHYLANAIPLLHRHNQPHTAKELYARLTETLP
                     DSVPKPSFDTVLTGWHPPQQ"
     gene            complement(9518..9796)
                     /locus_tag="PLZ74_06455"
     CDS             complement(9518..9796)
                     /locus_tag="PLZ74_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97998.1"
                     /translation="MISYWKNPKEIYNDDGLVLIIGRYDHKNQDNGGHKALGIHWGGY
                     PKSRGVLSPCVIPETTRNAILSGLLHQAVINANDAQINAIVEAITFFR"
     gene            complement(9798..10112)
                     /locus_tag="PLZ74_06460"
     CDS             complement(9798..10112)
                     /locus_tag="PLZ74_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR97999.1"
                     /translation="MKLMMEHPATGIIKTAPVGFSWTTLFFSGFPALFRGDMKWFFIQ
                     LLCSMITWGLSAFVFMFIYNKLYIKKLLEKGFKVKAVEGGTMEQANYKLGINLPLFES
                     GK"
     gene            10377..10517
                     /locus_tag="PLZ74_06465"
     CDS             10377..10517
                     /locus_tag="PLZ74_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98000.1"
                     /translation="MNRERRNTMTTLSIVEARNRLAEAINRVSYGGERVVLARRGSRS
                     PH"
BASE COUNT         2095 a         2974 c         3315 g         2167 t
ORIGIN      
        1 taagccagga ttacaggatt aacaggattt ctgattgaag ttatcggtaa gccggccgtc
       61 cggggttccg ggcgagaacg gggtggtgac gggagcgtta aactgtaagc caggattaca
      121 ggattaaaac gctcaaggaa gaaaacttct ggtctgcggc atcttccaaa atcacccgcg
      181 cagcgggcca ttcttaatca tgtcaatctt gtcaatcatg tctctcaccc tttccaactg
      241 aaccctggcg cctgaatcct aggtgcggtg caacgaagta aaaccacagt ttcagtcacc
      301 gtatggctgt tttggtctgg aagcttgtcg gcctgttgct gtaaaatgac ggctacgtgg
      361 ttcttaacgc taagaagttt gaggttttca ggtgagaaag gaaaaggaaa acgacattga
      421 acttgggaag gtgctgacca aaggcatgac gttcacgtat gagtacgatt tcggatccac
      481 gaccgagctt gtcctgaaag tggtcggcgc atcacagaga catgacgtgc cgttggacgt
      541 cccgattgtg ctggcgcgga acgatccgcc cgcatgggta tgcgcggact gcggcaagcc
      601 ggtggtacgg gttgatgcca tgacgggata tggtgcctgc aaagacaccg ttttttgtgc
      661 ggcatgcgcc aaaaagcgga gaaaaacagg tgaatgctat tgcctcaaac tcgtcaattc
      721 tccgcgcacc ggtgtatgcg catatgagtg agcccttcag gcctcacttt aagaaaaaat
      781 gactcagggg ttgatatccg agttctgcca ttagtgcgac agtccctctt ctgcttctgt
      841 atcggccagc gtttctctca gccgcgcttt ttctgtactg ttgctttgcc cggcttcggg
      901 gaaaagagac atgaggcgat ggggcgtccc gccacgccca ggctgttccg cggatagacc
      961 ttcaaacgat agtccccgtt gtccggcaag gacatcccgt caaagtcaaa gcgcaacacg
     1021 tcggcatccc gccaatgggc acggtggaag gtcggcgaga ggaaacgctt gaccgccgct
     1081 acggttccgt ccgtcatctc ggcccgcacc tcatagtcga aagcccgccc cttggtcgct
     1141 ttggcagccg ggaactccag cgtcatgaaa atcgtccagc gtccgttccg ggtgtcgccg
     1201 ttgctcacaa atgtccggac agttgcccca ctgggaaact gcggaaccgg aacccggttc
     1261 cgctgcgcct cgaacgtata gggctttttg ccctctggca caatccaggg atcggcaatg
     1321 tccgtgcgct cgccaaagtc atggcgttcg atctccaccc gggtcggata gaccgagaca
     1381 aagaagccct gcggctgttc gaggttttcg cgtgaaggaa tccggcgcat tcccaacgtt
     1441 gagtcgcctt tgcggctgtc ggaaccgttg tcatatcccc ggggtgccga ggtgtagctc
     1501 agcgacggaa ccgaaatcgc cgtgaagcca tcctgccaaa tggacttttc atcattgagc
     1561 gtccggtgcg tgtgtccggt gaatacgaag ccattggcaa agccgtcgag cgttgttttc
     1621 agcggattgg gtttgtcatc tttggacgcg cccgccgcca cgcccggaat cggtatgtgg
     1681 cggaaaaaga aaaagggccg tccgggtgtc ttcaattcat ccgcatggtc gctgagccat
     1741 ttggcgatca tctcatcgcc gcaggttttg tttttccagt tgaactccgc gctgatgaaa
     1801 tggtaaccct tgatggtgcg gcggcgaatt tctccgaact gctctccgaa ggacttttcc
     1861 cagcattcgc ccattcccag atcacacaag gcgtcatcct cggaataacc ctgcatgtgc
     1921 atgtcgagcg tcatatcgcc gtaccaccag ccgtcgtagt catggttgcc cgtgcacatc
     1981 agcagctcga tgccggtccc ggccatctcg tctttccagg cttccgccaa atagtcatac
     2041 ccgcttttga gaccccagtc ggtaaggtcg cctgcgatca tgaccgcatc agcaccccgc
     2101 cgcctgaaat aacgtaacgc ctcgcgcagc cgggctgtcg attccggtgt ggtcacgtgg
     2161 gtgtcgctga tgaccccgaa cttgagatcg ggcctggctg cgaaccgccc cgtcgactga
     2221 cacccggcaa ggctgaatac agccgccgcg cccccgataa actcccttct tgtctgcatg
     2281 taacgccctc cttttagagc aaagcggaag cgatgcccag ataaatgaac acccccagaa
     2341 tgtcggccag cgaggtcacc agcggactgc tggccacggc ggggtcgatc ttgagcttgc
     2401 gcagaatgaa cggtaaggtc atgccgatca agctgccgct caccacgatc agagtcatgg
     2461 atagcgcggc caccacaccg atacgcaggc cgccacgcca ccaggccaaa acgaacaccg
     2521 ccgccgacat ggagatcccg aggctcagcg aaaccacaat ctcgcgccat aaaaccttca
     2581 tgaaatcctt cagcgtcaat tcgccgaggg ccatgccgcg gatgaccagc gtggcggatt
     2641 gtgcgccagc gttaccgccg ctggcgatca gcagcggcaa gaagaaaacc aaggccacga
     2701 acgatccgat caaggtgtcg aagtgcgcaa tgcccgctcc ggaaaagata ttcacgaaca
     2761 ccagcgtcac cagccacggc aagcggctgc gatatagtat ggagagcgac gttctgaggt
     2821 agcccgcgct cagcggcatg atggccacac ccttatggat gtcttcggtg gcctcttctt
     2881 cggagacgtc gagaatgtcg tcgttcgtga cgatccccaa cagtacaccc tccgagtcga
     2941 cgaccggaag gacatgcagg tcgtatttct tgaacaccat gaccgcctct tcacggtctt
     3001 gcaaagagtg cagcgccaca tattggtggt ccatcaagga tgatataggc gtgtccggat
     3061 cagcgaggat cagtttgcgc agcaccagtt cgtctaccaa aacgccgcgc tcgtcaatga
     3121 tgtaaatcat catgacggtt tccgaatcat tgcccatggc gcggatttgt tccagcgttc
     3181 ttgcgacggt ccactcttca cgggcgaaga catagcggtt ggtcatcaaa cgcccaacac
     3241 tgtccttcgg gtagctgagc agactgaggg cctgtctgcg gtctttgtcg ctcaacagat
     3301 tcagcagccc ctgtaacacg ttggccggta actcctcgaa cagcgcggtc cggtcgtccg
     3361 gcgaaagcgc cgtgagaaca tgacgcgtct cgtcctgcgc catattttcc agcaacaggt
     3421 tctgggaggg gtattcgagc agggagaaga cctcaccggc catgcttcgc ggcaacaggc
     3481 ggaacaccag caactggtcg gatgtctgaa gcgtcgcgat caactccgcg atttccgggg
     3541 tgggctgcgc agcgaggaaa atgcgcaggt cgttaaaccg ccgatcctcc aacatctcga
     3601 tgatgtcggg catcagcagt tggctgagga ctgtgctggt ggcgcggcgt acctgctcgc
     3661 ggtcatcctc tgcgtcctca cgtccctcaa ttgtcgcgat ccacgattca agacgggact
     3721 ggacgtcctc cagatctttg aggttaacca attcgtgctt ggtctcgtcg tcctcgatag
     3781 cccgggcaag cgtcgccaat tcagtcagat aggcgttacg ggcagagccg gggatgtagt
     3841 acatcaggat cagatgcaca ggccatccgt cagtgttacc gtaatcaatg ccgtcagggc
     3901 tccagcctat aacgcaagcc atctcgccgc catgctcttc cgagcgggcg tgcggacagg
     3961 cgataccata tttgaggtag gttatggcct gcttttcacg ccgaatgaca ttttcgaaaa
     4021 cgttgtactc cgccggtatg tcggcagagg cacaaagttt ttccgcaagc gtgcggagtg
     4081 cctgattttt gtcgcagggc ggcagctcga tcaaccgtcc ttcagcgata gctttgagaa
     4141 ttggaatcac tgcttttgac cggtaaaaag gatgaagggt agactgaaaa caaacgggtt
     4201 cccacttcca cgattggcag tcattatgcg aaagggaagg gaagagagtc aaccccgaaa
     4261 gtcaacggat cgcgacggga acagttcggt ttggcggtat gcggcttggg caccgggtgg
     4321 cggggtaggc gtcgcgctgg gcggcggggt ggggggcgtg ttttgcggtg tgccgccgag
     4381 cagactttcc agtccggcct cgtccaggat ttccgtgccg agcgcacgcg ccttgtcgca
     4441 tttcacgcct cccgcgtctg ttcccgccac cagatagcgg gtctgccggg tgacggtgtc
     4501 ctgcaccgta ccgcccgcgg tccggatccg ggcggcaagg gtgcgacgcg gctgcgagag
     4561 cgttccggtc aggacgaagg taagcccggc aagcggtccc tccacggcgg ccggtttggg
     4621 ccgggcgcac gggtcaatgc cgaggtcctt caagcgctgg aggtagcgcg cgccgtattc
     4681 ggaggcaaag aaggcgagga ccgccttggc ggcttcgggt ttgatcttgc aggtgtagta
     4741 tggcggcagg gtgctgccgg gcgtccgggt cgcggcgtcc gccgcgacca gcgcgtcacc
     4801 cgtcacgccg atctcaccgc agatgcgatt gaagaggttc tgccgctctt cgcgttcggc
     4861 ctcgtccttg ggccggttgg cggtcgagcg cgggttgacg gttgtggctt gggcataaag
     4921 ctcgtccaat ctgagcacgg cttgaaggat tggtgattcc gcaaggctgg agaagttctt
     4981 atgcacggat gcgatctgtt ctgccacggt cacaccgatg ctggggatgc cggtcgcaaa
     5041 gagccaacgg tccaacggca gggtgcgggc tgcttccaga gattgtagcg cggtctgcgc
     5101 gttcttgccg aatttgcgcg tgccgctctg ctcatcgccg atgcggaagg cctccagttg
     5161 cggcagggtc aggtcgaaga gatcgagcgg atcgcgtacc cattcttgcg cgaccagggc
     5221 ttcggcgacc ttgccgccga tgccgcggat gtcgagggcg tcacgcgagg cgaaatgttc
     5281 gaggcggccg acgcgctgcg cgggacaggc gggattggtg cagcggtggg caacttcgcc
     5341 ttccagacgc acgaccgcgc cgccgcattc gggacagacg gacggcatga cgaagacgcg
     5401 ctccgtgccg tcgcgctctt cggtctgcac gctctccacc gccgggatga catccccggc
     5461 cttgacgatc cagacgcggt ctccgatgcg gatgtcttta cgggcgatct catccgcgtt
     5521 gtgcagggtg gcgcgggcga tttccgaacc ggcgagcaat acgggcgtga gttccgccac
     5581 cggtgtgagc acaccggtgc ggccgacctg cacggtgacg gatccgatgc gcgtggtggc
     5641 gcgttccggt tcgtatttga aggcccgcgc ccagcgcggg gctttggcgg tgctgccgag
     5701 ccgggcgtac agatcgcggc ggttgacctt gatgaccgcg ccgtcgatct cgaacgggaa
     5761 cgcgtgccgc aggctttgca gttcgtcgat agcgtcgaga accgcctgga tatccacaca
     5821 gagccgttgc caaggcgggg tgcggaaacc ccattccgcg aggcgcgcga gcagaccgcc
     5881 gtgggtcggg aactccacgc cgctaccgtc gaccgccgca taaagcacgg cgtcgagcgg
     5941 acggcgcgcg acttcgcggg ggtcgagctg tttgagtgat ccggctgcgg cgttccgggg
     6001 gttcatgaag ggctcgcgac ctgcctcctc catgcggcgg ttgagcgcgg cgaacccctc
     6061 gcgcgtcatg tagacctccc cgcgcacttc aagcagcgag ggggcgtccc ggaggacgag
     6121 cgggatcgag cggagggtcc ggatattcgc ggtgatgtca tcgcccacct cgccgttacc
     6181 gcgcgtggcg gcacgcgtca gggcaccgcg ggcatacacc agcgaaaagg ccacgccgtc
     6241 gattttgggt tccaccacgt aatcccagac ctcatcgggc atctggcggc gcaggaaggc
     6301 gtcgaaatcg gtcaggtcgc cgcgcgagtg cgttttgtcg aggctcatca tcggcggatc
     6361 gtggcggacc tgccggaagg cgtcgagggg ggcaccgccc acgcgttggg tcggggagtc
     6421 gggggtgacc cattcggggt gcgcgcgttc gagcgcttcg agttcgcggt acaacgcatc
     6481 gtaatccgca tcaccgattt cagggcgcgc ctccacatag tagaggtggt cgtgccgccg
     6541 gatttcggca cggaggtgtt ccaggcgttc gcgattcatg ttacggtctc cggggttgtt
     6601 acgacggggc gggacgcgga ttgagcagtg cttcgatgtg ggatcgctgc tcatccgccg
     6661 cgcggacccc ggcggcgatc ccttcgagcc cgcggtggaa ttcgagggtg agcaggttgc
     6721 cgacctcggg ctgaatcagg atgtcgggcg ggtggagcgc gagttcgtgc cgcgtgatct
     6781 ggttctccac aagacgcatg gtgtgggtga gcacgtcaaa aatcgagagc gggtctgcgt
     6841 tgtgtttgcg cggggcggtc cagcggtgcg ccaattcgga catgggcgtc tgcagggcgt
     6901 ctggcatgag acgcgaaatc gaggcgatca gcgcgtcgac atgcgggttc ggggcgtcgg
     6961 cgggtttacg tggcgtgcgg cgcggccgga ggggcggggt acggagattg acatccaccg
     7021 cgatcacgat ctcggccccc agggctcggc agaccgagac cggaagggga ttgacgagcc
     7081 cgccgtccac cagggtttgg ccgttgagcc gcgtgggtgt gaagatgccg gggatgccga
     7141 tactggcgcg gatcgcgtcg aacagattgc cggaggagag aaccacctcc ttttcggtgt
     7201 ggaggtttgt ggcgaccgcc gcatacggga aaggcagatc ggcgatattc cgcgcgggga
     7261 tgatgtcttt gaggagccgc atgaagtttt tgccggtgat caggcctgag cgcgggaacg
     7321 tgacttcaaa gaacagttgc gcgagctgtt tccaatccag gcgggcagcg agcgcctccg
     7381 tggtgtcgag cgcgttgcag gcgtaaagcg cggcgacgat ggcgccgatg ctggtccctg
     7441 ccacacagac gggccgcacc ccgaatgcgt ccagtcggcg cagcaccccg agatgagccc
     7501 agccgcgcgc accgccgctg ccgagcgcga ttccgatacg aggggagttg cggggagggg
     7561 gttgtatgcg cttccgtgcg ttcatttcgt tttccgggct gaggggaagg aggaggtggc
     7621 tttgcggttc ttcacaaaaa ccgtgcggga gtaaccgccg cgcagcaact cggcggacac
     7681 cgcgcgcatg agcgcccggt ccggttggtc cggaatcagg atgttcacct cttggctggt
     7741 acggatgccg ccggaacgca aggctgaaac aagtttgccg ggttcgaccg gttcttggtt
     7801 gaaccggatc gtgccggact cctcgatggt gatttccggt acgcgggtgt tttcaatgac
     7861 tctcgatgtg gtgcagccgg atccgccgag cataatgagg gtcacgctga gcacggccgc
     7921 aaaaaagcgt atcgtcatca tctctcctga cttggtttcc cgctgttgcg ggtatggatg
     7981 acaaaaattt taccacagaa agccgatttg cggtaaacaa aggattccgc gacgggttat
     8041 tgctgggggg ggtgccagcc ggtgagcacc gtgtcaaatg agggtttcgg tacgctgtcc
     8101 ggaagcgttt cggtcagacg cgcgtagagt tctttagcgg tatggggctg gttgtggcgg
     8161 tgcagcagtg ggatcgcgtt ggccagataa tggacgcaga tgtttcgctg tgtcgcggtg
     8221 gggtgctcgg aaagcgtctc aatcaaaaag gccgccgtgg cggaagccag cgcggggttg
     8281 ggtgcggtct gccactccgg gccctccgga tcgccagtga ggcgtccgcg ccagaccgaa
     8341 agcaggagcg gctggtaaac ggcgcggcgg cagagcaggc gctcggttcc ggtggccaga
     8401 tcgagtcctt gggtcgccca gtagaccgca tgactctcgg caagacgcca gtcgagcggg
     8461 ccgaagcggt tttccagcgc gcgcatgcgg tcggcatcga ggcgcatcgc ggccagcgcg
     8521 gagcgggact cctctgtaat ccgcaccgtg ccgtcgggat tcacatgggg agagaggagg
     8581 gtggcgaggt ggcgcttgta ggtcaggtgg gcgctgtcca gcgaatcccc gatcttgttc
     8641 tggtagagcc aagccagttc gcggtacagg cgcgcctcgc gggggttgaa ccgcagggcg
     8701 tcatcgcgca gcagggtcaa tccgttccgc acccagcgca ggcgatcctc ttcccggatc
     8761 atcaggatgc tgacgttgta ggccaaattc cacgcattgt agatccacgc gtccgcggca
     8821 tgggggtcga gccgggtgat ccagtcggaa agttgaacca gttcgaggaa acgtccctcc
     8881 tcctgcagcc ggttgatacg gaaccaaagc gcttccgccg caatgccgcg aaacccgccg
     8941 aggccgacca gaaggaaggt gagggccggc ggcggccttt cggccgagcc cgcataggtt
     9001 tcgggcatgc tctcccgacg ctgcacggcg agcctgcggc tttgccagac cgctgctacg
     9061 gtcagcagaa aacacattac gatgggaagc caaacgcgca tgaggttttt gtatcacaat
     9121 cacctcctgt gttgacagtc tgtaatttaa ccatgaaaca caagaaacgt catgaaaaac
     9181 ggggggtgcc ttttcgtgtc gtgtattccc gcacgggcgc gggcacgttg cgccaacagg
     9241 tcaggtcgtt gaggcgcacg tcgaaagaac gtcgagctct gaacattgaa gtttccttct
     9301 gcgttgggcg ttctgcgttc ctctccttgt tgcgaccatg cagtggctcg cgaccgtgag
     9361 agtaaacccc caaagagttg aaccttcaaa gctcttaggt ttcatggttc atccaaatcg
     9421 gggtcgctat cgggatcgat tgtttaacca cggaacacag aatacacggg aaggcatggc
     9481 tctgctttcg tgtggtttgt gccgttcgtg gcttaaccta cctgaagaac gttatggcct
     9541 ctacaatcgc gttaatttga gcatcgtttg cattaatcac cgcttgatga agcaaacccg
     9601 agaggatagc gtttcttgtt gtttctggta tcacacaagg acttagcact cctcgtgatt
     9661 ttggatagcc tccccagtgg atgccaagag ctttgtgtcc gccgttgtct tggttcttat
     9721 gatcgtaccg gccgatgatc aacaccagcc catcgtcgtt gtagatttct ttcggattct
     9781 tccaatacga gatcatatta tttcccgctt tcgaaaagag gaaggtttat tcccagttta
     9841 taattcgctt gctccattgt gccgccttca accgctttga ctttgaaccc cttctcaagc
     9901 aacttcttga tatacaattt gttgtagata aacatgaata caaacgctga tagaccccat
     9961 gtgatcatcg aacaaagaag ctgaataaaa aaccatttca tgtcgccacg aaataatgct
    10021 gggaagccgc taaagaataa ggtcgtccat gaaaaaccca cgggggctgt ttttataata
    10081 ccggtagctg gatgttccat catcagtttc atgcgttttt cctaatgtcg ccgttttgat
    10141 tgtagagtag aaatgtgcga cgccacttca ttctcactgc cacgggcaaa ccggcaaccc
    10201 accggttctc atggttggca cgtattgtgc ggggggggga taaaagtcaa ccctaaaaaa
    10261 acatgaaaaa gtggggtgcc tagcacctag ggctaccatc taacacgcag cgaacggaac
    10321 taaaatagga ttgaaggggt attgacccgc aacgcgcggt tatgtgcgtt atggacatga
    10381 atagagagag acgaaacacc atgaccacgc tcagcattgt cgaggcgcgc aatagactgg
    10441 cggaggcgat caaccgcgtt tcttacgggg gcgagcgggt tgtcctcgcc cggcgcggaa
    10501 gccggtcgcc gcactgattt cgaccgaaga ccttgccctg ctccagaagc t
//
LOCUS       DAOSRB010000093        10324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6313_length_10324_cov_7.446392, whole genome shotgun sequence.
ACCESSION   DAOSRB010000093 DAOSRB010000000
VERSION     DAOSRB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10324
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6313_length_10324_cov_7.446392"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..2122)
                     /gene="aspS"
                     /locus_tag="PLZ74_06470"
     CDS             complement(332..2122)
                     /gene="aspS"
                     /locus_tag="PLZ74_06470"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOR98001.1"
                     /translation="MHLYRSHTCNELRTTDAGTTAKLSGWIFRIRDHGGILFIDLRDH
                     YGITQVVVHPNRPFFEPLSKAKLESVITVTGEVTLREPSTINPDIPTGEVELVANECL
                     IEAASEQTPLYLPGEEDGAEDLRLKYRFLDLRRQRIHNNILLRSRIISFLRKAMEAHG
                     FNEYQTPILTCSSPEGARDYLVPSRVHHGKFYALPQAPQLFKQLLMVAGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDMEMSFVTQDDVFAVVEDVLHKTFTAFSKKHVDATPWIR
                     IPYREAMLKYGTDKPDLRIPIEMFDCSEIFKTSGFNAFAAAVKSGAVVRAIPVKGIKD
                     KPRSFFDKLVEHAKTLGSKGLAYLVWDGDTVKGPIQKFLTPEELAALASIADAKDGDV
                     IFFVCDEADTAAKIGGDIRAKLGRDLELIDKNVFRFCWIVDFPMFEKDPETGATAFSH
                     NPFSMPQGGMDALLNKDPLDILAYQYDVVCNGIELSSGAIRNHLPEIMYKAFEIAGYG
                     PEVVEEKFSCLLNAFKFGAPPHGGIAPGVDRMVMLLAGEDNIREVIAFPMNQKAQDLM
                     MNAPSEVTEKQLRELHIKIRGREQPAPVTG"
     gene            complement(2200..3129)
                     /gene="era"
                     /locus_tag="PLZ74_06475"
     CDS             complement(2200..3129)
                     /gene="era"
                     /locus_tag="PLZ74_06475"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOR98002.1"
                     /translation="MSPSAPHGSKRCAVVGIVGRTNSGKSTLLNRMVGEKVSIVSPVM
                     QTTRSTIRGILTEDRGQLVFLDTPGLHKSEGTLGTLMNRMARQASAGVDLLLVVFDGG
                     NRPHLEDDGWMRRVLFAEHPVVFVLNKSDRSPFYETAFKAQWETVQTEKQMRREVVWV
                     TTSAIHPAGAVDLVDRIFALATPSDHYLFPEDVVTDYPRKLTIADVIREKFLVRLYEE
                     VPHELGVKVENIDEQPHGWTVSVTIYVNRPSQKGLVIGPKGRTLKYVRQCAEPELSDL
                     FGVKVVLDLWVKVEKDWMKNFWLLRQMGYAGTH"
     gene            complement(3126..4496)
                     /locus_tag="PLZ74_06480"
     CDS             complement(3126..4496)
                     /locus_tag="PLZ74_06480"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOR98003.1"
                     /translation="MTLIEKILSNPQAYSLDVETLGECKIESPIRNREFIDTEERILI
                     TENVKKLKYVTDRLGTPPSFERAGPHQKIFHDPTWSRVGILTAGGLCPGLNHVIKGLV
                     EILMFDYGIKTIYGIRYGYAGLIPRYGYQPIMLNTDTVDTIHEQGGTLLGSSRGQQDT
                     GELVDALARMNINLLFCIGGDGSLRCARDIAEECKRRKLAVSVIGIPKTIDNDLHFVG
                     RSFGFETAVAEATQIIQAAHTEAKGTFNGIGLVKLMGRDSGFIAAYATLANPVVNFCL
                     IPELDFELDGPHGLLAALTYRFERFKDHVVIVVAEGAGQRHITDQPERRDPSGNILKK
                     DIGEYLHHRIRNHFAKLRREASVKYFDPSYTIRSVPAKGTDAIRCYMLARNAVHAAMA
                     GRTNCVVGNQNDWYTLVPIRLATINRQKVNLNSDLWRAVLDATGQALYFNGDSANGML
                     NGEAVP"
     gene            4608..4683
                     /locus_tag="PLZ74_06485"
     tRNA            4608..4683
                     /locus_tag="PLZ74_06485"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4641..4643,aa:Lys,seq:ttt)
     gene            complement(4764..5732)
                     /locus_tag="PLZ74_06490"
     CDS             complement(4764..5732)
                     /locus_tag="PLZ74_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOR98004.1"
                     /translation="MKKVLCLGLSPVLQRTLRFDRLTPDEVNRARQVMESAAGKALNT
                     ARALVTLGTPAITAGFNGGASGARVTAMLKQCGVGDAMTTMPSPTRLCTTLLDDHAGT
                     VTELVEEAPAPGRTALRRFIADNLSRIPGAALLAISGTLPPFAKDDFYVAFVRAARAA
                     GVPVVIDSHRTALIQVLFEQPLLAKLNVAELEATLGEKLNTEARILRGMRDLLNLGAA
                     NVFVTQGSRAAYLLTPRAAWRFTPPAIRERVNPIGSGDCTTAGIVHALLRGKRLPDAV
                     RLGLACGSANAETLTPGTFDARRARALCRTVTCEPRMVRETVPQKN"
     gene            complement(5729..6367)
                     /locus_tag="PLZ74_06495"
     CDS             complement(5729..6367)
                     /locus_tag="PLZ74_06495"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOR98005.1"
                     /translation="MFTGLVQRMGVIRRLERSGAGWSLAITHDPWPDALVIGESVAVQ
                     GACLTVTALLPDGFTADLLDETLQRTALQRLGPGARVNLERALALGDRLGGHLVSGHV
                     DECGTLLSVEARGRDAVWRVRCSPELASHTVRKGSVTLDGVSLTVTNVGENWIEVNLI
                     PHTLRITTLGDRQLGDALNVEGDLLGKYVARLLGRDTRGGLTAQMLEENGFI"
     gene            complement(6371..7468)
                     /gene="ribD"
                     /locus_tag="PLZ74_06500"
     CDS             complement(6371..7468)
                     /gene="ribD"
                     /locus_tag="PLZ74_06500"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOR98006.1"
                     /translation="MRDDRYWMRRALTLARLSEGRTRPNPPVGAVVTRAGRMLGAGRH
                     RRAGCAHAEIEALDACRTSARGATLYVTLEPCCTHGRTPPCTDRILREGITRVVVGCR
                     DENGCHCGRGLSLLQKQGVEVVCGVCEEEARELALPFFTRVTTGLPFVTLKLALTLDG
                     RIADRIHRSQWITGEAARVEVQRMRRRADAVMVGAGTVCADNPSLLCRLRHPDGAKLL
                     RVVVDATGRTPPDAQVFSDAAATRTLVATTPDGAARRGAAWAQHGARVWTFAPDKAGR
                     VPIRRLLRKLAAEGCLHVLCEGGGRLAGSLHDADAVDEYSFFYAPAVLGDSDAIGAVM
                     GCGNLLAGMRRLRCMEVNRFGADIRIRARRA"
     gene            complement(7489..7980)
                     /gene="nusB"
                     /locus_tag="PLZ74_06505"
     CDS             complement(7489..7980)
                     /gene="nusB"
                     /locus_tag="PLZ74_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOR98007.1"
                     /translation="MATRRQGREWALQMLFQADLNPGLDLNLTIPKFWTQQYTCKLEE
                     AADKDLTLPEKPDRSVEDRVAPPKIREFSERLVRGVLDHLPELDAKLTSYTKNWPLHR
                     MGSVERNVLRLAFYEMLYCADVPPAVVLNEAIDLAKYFSNAEAGRFVNGVLDRLNKEL
                     NRT"
     gene            complement(8017..8478)
                     /gene="ribH"
                     /locus_tag="PLZ74_06510"
     CDS             complement(8017..8478)
                     /gene="ribH"
                     /locus_tag="PLZ74_06510"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOR98008.1"
                     /translation="MVEITGQLDAKDMRLALVVSRFNHFFTEQLVKGAVDCFLRHGGK
                     EDALTLVRVPGANELPLTAQRLASAGKHHVVVVLGAVIQGATPHADLINGTVARALSK
                     IALDTGVPVINGVVCAQNLEQAVERAGTKHGNKGWDAVLAAIETVNVLRAL"
     gene            complement(8565..9773)
                     /locus_tag="PLZ74_06515"
     CDS             complement(8565..9773)
                     /locus_tag="PLZ74_06515"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOR98009.1"
                     /translation="MEKDSVFDPVETCIEAFGRGEMVVVADDENRENEGDLIIAAEKI
                     TPEAINFMVVNARGLVCVPMTGARLRELGVERAPSRNRGDKFATAFTYSVDAATGVTT
                     GISAADRAWTVKTLLDPASGPESIVSPGHIFPLEAREGGVLVRAGHTEAAVDLAKLAG
                     LKPAGVICEIMLPDGSMARLPDLREFATRHGLKMLSIADLIQYRRHRERLIDLVEEVE
                     LPTAYGQFTLRLYVSSPDGREHLALIKGNLAKADAPLVRVHSECLTGDVLGSLRCDCG
                     NQLHAAMRRIDQEGCGVVLYMRQEGRGIGLANKLHAYKLQQNGFDTVEANIELGFPAD
                     LRDYGVGAQILADIGLKRIRLMTNNPKKLIGLQGYGLEIVERVPVVFEPGTHNRRYLE
                     TKKNKMGHLI"
     regulatory      complement(9835..9979)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         1877 a         3144 c         3295 g         2008 t
ORIGIN      
        1 cgcagggctg acgtattgag atgcgggcga agcccgcaac ctcaaaggcg gtgaggctgt
       61 gaggctgtga ggctgtgagg ctgtgaggct gtgagactgt gaggctgtga gactgtgagg
      121 cggtgagact gtgaggcggt gagactgtga ggcggtggtg aaacagtcga tcccgatagc
      181 gatcccgata gcgatcccga ccccgatagc gatcccgata ccgatagcga tcccgacccc
      241 gatgaaacct tgaaacccag aaaccttgaa acccagaaac cctgaaaccc cgaaaccaaa
      301 gagagttgaa ggttcaactg ctctttttag gttaaccggt gacaggcgcg ggttgctcgc
      361 gtccgcggat tttgatgtgg agttcgcgca gttgtttttc ggtgacttcg gagggcgcgt
      421 tcatcatcag gtcctgcgcc ttctggttca tggggaaggc gatgacttca cggatgttgt
      481 cctcaccggc cagtagcata accatgcggt cgacgccggg ggcgatgccg ccgtggggcg
      541 gtgcgccgaa tttgaaggcg ttgaggaggc aggagaactt ctcttccaca acctcggggc
      601 cgtaaccggc aatctcgaag gctttataca tgatctctgg gaggtggtta cggatcgcgc
      661 cgctggaaag ctcgatgccg ttgcagacca cgtcgtactg gtaggcgagg atgtcgagcg
      721 ggtccttgtt gagcagcgcg tccatgccgc cctggggcat ggagaagggg ttgtgcgaaa
      781 aagcggtcgc gccggtctcg gggtctttct cgaacatcgg gaagtcaacg atccagcaga
      841 agcggaagac attcttgtcg atgagttcga ggtcgcgtcc cagtttggcg cggatgtcgc
      901 cgccgatctt ggcggccgta tcggcttcgt cgcagacgaa aaagatgacg tcgccgtctt
      961 tcgcgtccgc tatggaggcg agagccgcaa gctcttcggg cgtgaggaat ttctggatcg
     1021 ggcctttgac ggtgtcgccg tcccagacga ggtaggcgag gcctttggag ccgagggtct
     1081 tggcgtgctc gacgagctta tcgaagaagc tgcggggctt gtccttgatg cctttgaccg
     1141 ggatcgcgcg gacgacggca ccggatttca cggcagcggc gaaagcgttg aagccggagg
     1201 tcttgaagat ctcggaacag tcgaacatct cgatggggat gcgcaggtcg ggcttgtcgg
     1261 taccgtattt gagcatcgct tcgcggtaag ggatgcggat ccacggcgtg gcatcgacgt
     1321 gctttttgga gaaggcggtg aaggtcttgt ggagcacgtc ttcgaccacg gcgaagacgt
     1381 catcctgcgt gacgaacgac atctccatgt cgagctggta aaactcgccg ggagagcggt
     1441 cggcgcgggc gtcctcgtcg cggaaacagg gtgcgatctg gaaatagcgg tcgaaccccg
     1501 cgaccatcag gagctgcttg aagagctgcg gagcctgcgg cagggcgtag aacttaccat
     1561 gatgcacgcg gctgggcacg aggtagtcgc gcgcgccttc gggcgagctg cacgtcagga
     1621 tgggggtctg gtactcgttg aacccgtgcg cctccatcgc cttgcgtaga aaagagatga
     1681 tgcgggagcg caggaggatg ttgttgtgga tgcgctggcg gcgcaaatcg aggaagcggt
     1741 acttgaggcg cagatcctcg gcgccgtcct cctcgccggg caggtagagc ggcgtctgct
     1801 ctgaggcggc ctcgatgagg cactcgttgg cgacaagctc gacctcgccg gtggggatat
     1861 ccgggttgat ggtcgaaggc tcgcgcagcg tcacttctcc ggtcacggtg atcacgctct
     1921 ccagcttggc tttggacagc ggttcgaaga acgggcggtt cgggtggaca accacctggg
     1981 tgatgccgta gtggtcgcgc agatcgatga acaggatgcc gccgtgatcc cggatccgga
     2041 agatccagcc ggaaagcttg gcggtcgtgc ccgcgtcggt ggtgcggagt tcgttgcagg
     2101 tgtgtgaacg gtacaggtgc atggtttcgg tttccttaaa aagtggtaaa acgataacaa
     2161 aacggcgcac gggatgccac gagggatttt tgcgcgggct cagtgagtgc ccgcgtatcc
     2221 catctgccgc aggagccaga agttcttcat ccagtccttc tcgactttca cccacaaatc
     2281 gagaacgacc ttgacgccga agagatcaga caactcgggt tcggcgcact gccgcacata
     2341 tttgagtgtc cggcctttgg gtccgatcac gagacccttc tgcgaggggc ggttgacata
     2401 gatcgtgacc gacaccgtcc agccgtgcgg ttgctcgtcg atgttttcca ctttgactcc
     2461 cagttcatgc ggcacctcct cgtacagccg gaccagaaat ttttcgcgga tgacatcggc
     2521 gatcgtcagc ttgcggggat aatccgtgac cacgtcctcg ggaaacaggt agtggtcgga
     2581 gggcgtggcc agggcgaaga tccggtcaac cagatcgacg gcgccggccg ggtggatcgc
     2641 agaggtggtg acccagacga cttcacgccg catttgcttt tcagtctgaa ccgtttccca
     2701 ctgcgctttg aaagccgttt cgtaaaaagg tgaacggtcg cttttgttga ggacgaaaac
     2761 gaccgggtgc tcggcgaaca gcacgcgccg catccaaccg tcatcctcca gatgcgggcg
     2821 gttgccgcca tcgaacacga ccagcagcag atcgaccccg gcggaggcct ggcgggccat
     2881 gcggttcatg agcgtcccca gcgtgccttc cgatttatgc aggccgggcg tgtcgagaaa
     2941 cacgagttgg ccgcggtctt cagtcaagat gccgcggatc gtggagcgcg tggtctgcat
     3001 caccgggctc acgatgctca ccttttcgcc gaccatgcgg ttgagcagtg tggacttgcc
     3061 ggagttggtg cgcccgacga tgccgaccac ggcgcagcgc ttggatccgt gcggggcgga
     3121 aggactcatg gcaccgcttc cccgttcagc atgccgttgg cggagtcgcc gttgaaatag
     3181 agcgcctggc ctgtggcgtc caacacggcg cgccagagat cgctgttgag gttcaccttc
     3241 tgcctgttga tggtcgccag acggatcggc accagcgtgt accagtcgtt ttggttcccc
     3301 accacgcagt tggtgcgtcc ggccatggcg gcatgcaccg cattgcgggc cagcatgtaa
     3361 cagcggatcg cgtccgtgcc tttggcgggc acgctccgga tggtgtaact ggggtcgaag
     3421 tatttgaccg acgcctcgcg gcggagtttt gcgaagtgat tccggatgcg gtgatggaga
     3481 tattcgccga tgtccttttt gaggatattg cccgaggggt cgcgtcgctc gggttgatcc
     3541 gtgatatggc gctgtcccgc gccttccgcc accacgatca ccacatgatc cttgaagcgc
     3601 tcgaaacggt aggtcagcgc ggccagcaga ccatgcggcc cgtccagttc aaaatcgagt
     3661 tcgggaatca ggcagaagtt caccaccggg ttggccagcg tggcgtaagc cgcgatgaac
     3721 cccgaatcgc ggcccatgag tttcacgagc ccgatgccgt tgaacgtgcc tttggcctcg
     3781 gtgtgcgctg cctgaatgat ctgcgtggcc tctgccaccg ccgtctcgaa tccgaaactg
     3841 cgtcccacga agtgaaggtc gttgtcgatg gtcttgggga tgccgatcac gctaacggcc
     3901 agtttccgac gtttacactc ctcggcgatg tcgcgcgcgc aacgcagtga tccgtcgccg
     3961 ccgatgcaga agagcaggtt gatgttcatg cgggccaagg cgtcgaccag ctcgccggta
     4021 tcctgctggc cacgggaaga gcccagcaag gtgccgccct gctcatggat cgtgtccacg
     4081 gtgtcggtgt tgagcatgat cggctgatag ccgtaacgcg ggatcaatcc ggcatagccg
     4141 tagcggatgc cgtagatcgt cttgatcccg tagtcgaaca tcaggatctc caccaggccc
     4201 ttgatcacgt ggttcaggcc cgggcagagc ccgccggccg tcaggatgcc gacgcggctc
     4261 caggtggggt cgtggaaaat cttctgatgc ggtccggcgc gttcaaagga ggggggggtg
     4321 ccgaggcggt ccgtgacgta cttcagcttc ttgacgttct ccgtgatcag gatgcgctcc
     4381 tccgtgtcga tgaactcgcg gttgcggatc ggtgattcga ttttacattc accgagcgtc
     4441 tcgacgtcga gcgagtacgc ttgcggattg gagaggatct tttcgatgag cgtcatgacg
     4501 gttgttccct gttacggcgt gaagcgtacc attatacgtg attcgtgggg atggtcaatt
     4561 gacacccgta cggatttctg atttaatccc actcttcctg atgccattgg accatagctc
     4621 agtcggtaga gcacgacact tttaatgttg gggtcgtggg ttcgaatccc actggtccaa
     4681 ccatttcgca tggatgaaaa agacgtccgt ccctgggcga aaacggggag atacccggaa
     4741 cgctgaacat cgacctgcga cggtcagttt ttttggggga cggtttcccg aaccatccgc
     4801 ggctcgcacg tcacggtccg gcacagggcg cgcgctcggc gggcgtcaaa cgtgccgggc
     4861 gtcagcgttt cggcattggc gctgccgcag gccagaccaa gccgcacggc atccggcagg
     4921 cgtttgccac gcagtagcgc atgcacaatg ccggcggtcg tgcagtcgcc gctgccgatg
     4981 gggttcacgc gttcgcggat cgcgggcgga gtgaagcgcc acgcggcgcg gggcgtcagc
     5041 agatatgcgg cgcggctgcc ttgggtcacg aagacgttgg cggcacccag attcagtaga
     5101 tcccgcatgc cgcgcaggat gcgcgcctcc gtgttcagtt tttcgccgag tgtggcctcc
     5161 agttccgcaa cgttcagttt ggccagcagc ggttgttcga acagtacctg gatcagcgct
     5221 gtacggtgcg aatcgatcac caccgggact ccggcggcac gcgcggcgcg tacgaacgcc
     5281 acgtaaaaat catcctttgc gaaaggcggc agcgtgccgc tgatggccag cagggcggca
     5341 ccgggaatcc ggctcaaatt gtccgcgatg aaccggcgca aggcggtgcg gccgggtgcg
     5401 ggcgcctcct ccaccagttc cgtcacggtc ccggcgtggt cgtcgagcag ggtggtacag
     5461 agacgcgtgg gggagggcat cgtcgtcatc gcatcgccga ctccgcactg tttcagcatg
     5521 gcggtgaccc gtgcaccgct cgcgccgccg ttgaatcccg ccgtgattgc aggggtgccc
     5581 agagtgacga gggctcgtgc ggtgttgagc gccttgcccg ccgcgctttc catgacctgg
     5641 cgtgcgcggt tgacctcatc cggcgtaagc cggtcaaacc gtagggtacg ttgcagtacc
     5701 ggggacaatc cgagacagag gacttttttc atatgaatcc gttttcttcc agcatctgcg
     5761 cggtgaggcc accgcgggta tcgcgcccca gcaggcgcgc cacatatttc cccagcaagt
     5821 cgccctcgac gttcagcgca tcgccgagtt ggcggtcgcc gagcgtggtg atacggagcg
     5881 tgtgggggat caggttcacc tcgatccagt tctcgcccac atttgtgacc gtgaggctga
     5941 cgccgtccag cgtgacggag cctttcctga ccgtgtggct ggcgagttcg ggcgagcagc
     6001 gcacacgcca gaccgcatcg cgtccgcgcg cttcaacgga aagcagcgtg ccgcactcgt
     6061 cgacatgtcc gctcaccaga tgaccgccca gacgatcgcc cagcgccagc gcgcgttcga
     6121 ggttgacgcg cgcgcccgga ccgagccgct gcagggcggt gcgctgcagg gtctcgtcga
     6181 gcaggtcagc ggtgaacccg tccggaagga gggcggtcac ggtcaagcag gctccctgta
     6241 cggccacgct ctcgccgatc accagagcat ccggccacgg gtcatgggtg atggcgaggg
     6301 accagcccgc gccagagcgt tccagacgcc ggatcacacc catccgttgc accaatcccg
     6361 taaacatggg ttaagccctc cgtgcgcgga tccggatatc cgcaccgaac cggttcacct
     6421 ccatgcaacg caaccgccgc atgccggcca gcaggttgcc gcatcccatg acggcgccga
     6481 tcgcgtcgct gtcgcccagc accgccggcg cgtaaaaaaa ggagtactca tccacggcgt
     6541 ccgcatcgtg cagtgaccct gcgaggcggc cacccccttc gcacagcaca tggaggcacc
     6601 cctccgcagc gagtttccgc agcaggcgcc ggattggcac acgcccggct ttgtccgggg
     6661 cgaacgtcca aacccgcgcg ccgtgctgtg cccacgccgc gccgcgccgc gccgcgccgt
     6721 caggtgtggt ggccacaagg gtgcgtgttg ccgcggcatc cgaaaacacc tgcgcgtcgg
     6781 ggggcgtgcg gcccgtggcg tccaccacca cgcgcagtaa ttttgcgccg tcgggatgcc
     6841 ggagacggca gagcagcgag gggttgtccg cgcaaaccgt accggcgccg accatcaccg
     6901 catcggcgcg tcggcgcatg cgttgcactt cgacccgcgc cgcctcgccc gtgatccatt
     6961 gcgagcggtg gatacggtcg gcgatgcgcc cgtccagagt caaggccaat ttcagcgtga
     7021 caaagggcag gccggtggtg acccgcgtga aaaaaggcag ggccagttcg cgggcctcct
     7081 cttcgcagac tccgcagacg acctccacgc cttgcttttg cagcagcgag aggccgcgtc
     7141 cgcagtggca gccgttctca tcccggcaac ccaccaccac gcgcgtgata ccctcgcgca
     7201 gaatgcggtc cgtgcagggc ggggtgcggc cgtgcgtgca gcacggttca agcgtcacgt
     7261 acagtgtggc accgcgggca gatgtccggc aggcgtccaa cgcctcgatc tcggcatggg
     7321 cgcatccggc gcggcggtgt cggcctgcgc ccagcatccg gcccgcgcgc gtgaccaccg
     7381 cgccgacagg cgggttgggg cgcgtccgac cttcgctcag gcgcgccagc gtcagggccc
     7441 ggcgcatcca atatctgtcg tcccgcatct cccgcgtcct cccgtgtgtc acgtccggtt
     7501 gagttccttg tttaaccggt cgagcacgcc gtttacgaag cgtccggctt cggcgttgct
     7561 gaagtacttg gcgaggtcga tcgcctcgtt gagcaccacg gccggcggca cgtcggcaca
     7621 gtacagcatc tcgtagaagg cgaggcgtaa cacattgcgc tcgaccgaac ccatgcggtg
     7681 cagcggccag ttcttggtgt atgaggtgag tttcgcgtcc agttcgggaa gatgatccaa
     7741 gaccccgcgc acgagccgct cgctgaactc ccggattttc ggcggggcga cgcggtcttc
     7801 caccgaccgg tcgggctttt ccgggagcgt caaatcctta tcagcggcct cctccagctt
     7861 gcaggtatac tgctgggtcc agaatttcgg gatggtcagg ttgaggtcca atcccggatt
     7921 caggtccgcc tggaaaagca tctgcagcgc ccattcccgc ccttgtctgc gtgtcgccat
     7981 ggagactcct tgcctcgcgt tccggttttc agacgcttac agcgcccgga gcacattcac
     8041 cgtctcgatc gcggccagaa ccgcgtccca tcccttgttg ccgtgcttcg tgccggcgcg
     8101 ttcgaccgcc tgttcgagat tctgcgcgca gaccacgccg ttgatgaccg gcacaccggt
     8161 atcgagcgcg attttcgaca aggcccgcgc caccgttccg ttgatcaggt cggcgtgcgg
     8221 ggtcgcgcct tggatgacgg cacccagtac cacgacgaca tggtgtttgc cagccgatgc
     8281 cagtcgttgc gcggtgagcg gcaactcatt cgctccgggc acccgcacga gcgtcagcgc
     8341 atcttccttg ccgccgtgac ggaggaagca gtcaacagcg cccttcacca gctgttcggt
     8401 gaaaaaatga ttgaaccggc tcaccaccag cgcgagccgc atgtcctttg catccaattg
     8461 tcccgtgatt tccaccatgg cgttctcctg taacgcgttc cgtgttttcg tttcccgcgc
     8521 cgtttccggc acgccgcatc ggacgccctg tccgggttcc gaaatcagat caggtgaccc
     8581 attttgtttt ttttggtttc gaggtagcgc cggttgtgcg tgccgggctc gaagacgacc
     8641 ggcacacgct ccacgatctc cagcccgtac ccttgcaggc cgatcaactt tttggggttg
     8701 ttggtcatga gccggatacg tttgagtccg atatcggcca gaatttgcgc gcccacgccg
     8761 tagtcgcgca ggtcggcggg gaagcccaac tcgatgttgg cctccacggt gtcgaacccg
     8821 ttttgctgca atttgtaagc atgcagtttg ttcgcgaggc cgatgccgcg gccctcctga
     8881 cgcatgtaca ggaccacgcc gcacccctcc tgatcgatcc tgcgcatggc cgcgtggagc
     8941 tggttgccgc agtcgcagcg caacgagccg agcacatcgc cggtcaggca ttcgctatgc
     9001 acgcgcacca agggggcatc ggctttggcg agattcccct tgatcaacgc gagatgctcc
     9061 cggccatccg gcgacgaaac atagaggcgc agcgtaaact gaccgtatgc ggtcggcaac
     9121 tcaacctcct caacgagatc aatcagccgt tcccggtggc gacggtactg gatcaggtcc
     9181 gcgatcgaca gcatcttgag cccgtgccgc gtggcgaatt cgcgtaaatc cggtaaacgc
     9241 gccatcgagc cgtccggcaa catgatctca cagatcacgc ccgccggctt gagcccggcc
     9301 agtttggcga gatcgacggc cgcctcggta tgaccggcgc gcaccagtac cccgccttcg
     9361 cgcgcctcca gcggaaagat gtggccgggg ctcacgattg actcaggacc ggaggcggga
     9421 tcgagtagcg tcttgaccgt ccacgcgcgg tctgcggcac tgatgccggt cgtcacgccg
     9481 gtggcggcgt caacggaata agtgaaggcc gtcgcaaatt tatctccccg gttgcggctc
     9541 ggtgcgcgct ccacgcccaa ctcgcgcagg cgggcaccgg tcatcggcac gcagaccagc
     9601 ccgcgcgcgt tgacaaccat gaagttgatg gcttccggcg taatcttctc cgccgcaatg
     9661 atcaggtcgc cctcgttctc gcggttttcg tcatccgcga caaccaccat ttctccccgc
     9721 ccgaaagctt cgatacaggt ttcgacagga tcgaacaccg aatccttttc catcgccgcc
     9781 acctcaaaaa aaacccggtc cacaaacggg accggggcaa aagcaacaca ggcttcacat
     9841 cttctttcat ccagactaga ctgtcggcca cggaatcgaa ccgtgtcagt ggcgcgcagg
     9901 aacagcgtgc cagtcgcggg ctataccgcc ggtcaggact ctcccgtaaa cacgggatca
     9961 ccttgccccg aagacgtttg aaacggcatc actgtaatcc tttggaccgg atttggcaat
    10021 accggaagtc aaaaagaacg ggcgaacgtt caacccaaaa ggagcagttg aacgggtaga
    10081 attccagcaa agccccgtaa acattgggtt ttgcggtgcg cccgacgctc tttttgaact
    10141 cttagtccct gtcgatcgtc gcgggtcgaa tgtcgcgggt cgcgggtcga cgttcccgat
    10201 cccgatagcg atcccgatag cgatcccgat cccgaccccg atttggatga aaccagaaac
    10261 cttgaaacca tgaaaccctg aaacctgaaa ccaaggagag ttgaaggttc aactgctctt
    10321 ttta
//
LOCUS       DAOSRB010000094        10285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6342_length_10285_cov_6.460117, whole genome shotgun sequence.
ACCESSION   DAOSRB010000094 DAOSRB010000000
VERSION     DAOSRB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6342_length_10285_cov_6.460117"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3774
                     /locus_tag="PLZ74_06520"
     CDS             <1..3774
                     /locus_tag="PLZ74_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98010.1"
                     /translation="ESTSPLTYFWYEGEGDDGEDLVPAGQGADFTTPPVTAASSYWVR
                     VVDALGQYTDSRAVRVMPAFEIRTAADLQKVGSGLDGWMPDAAYLVMNDIDASETAGW
                     NEGAGFEPIGWHDSFAGILDGQGHTVSRLTINRPDDGAVGLFGFLENGAFVKNVKLRD
                     CTVIGGSEVGCLAGTLYESTLQSCSVAGVVSGDEAVGGLVGWCGDSTVQSCYATGAVY
                     GQYNAGGLIGSLSDDSAVQSCYATGAVYGSYVTGGLVGGCGYSTVQSCYATGTVEGDE
                     GVGGLIGSLYDYSAVQSCYATGAASGQNFVGGLAGYCDFDGVVRTCYAAGAVDGMSGA
                     GGLIGYAEEAFIIEDSYWDTEASGLALSAGGEGRTTAHMQQQGTFTGWDFSAEWGIVN
                     GVSYPYLLSFPHPFSLTASTRGAGGVTAAPAADRHAPGAIVTLTAVAAPGQNEFVKWV
                     GAVSDPLSPVITVRMDGHKSVLAVFRTFRGLSSIADVQKIGSDPAYPSDGRYWLEQDI
                     DATETAQWNDEGTDESLMEGFRPINLFAGFFDGNGHTVHGLSINRAESAEGNGTALFS
                     VIERGAVVANLGLTDCLISGRYAIGALACENYGVIERCYASGAVMAPLSGSYAVYTNA
                     YDGYIWTNYINTSVGGFVCYNGGRLSECYAAVAVDPLAHDAGGFVLYREENERDVVER
                     CFWDTDVSGLDPEQSAGGTACSTLQMKQSATFLEWDFTEVWKIEEGIGYPSLRSFDTF
                     VIGLDASGAGDMPMLRLIAGRPFGNLPSPSRSGYAFGGWWTGPGGTGEQVLPTDIVPG
                     ADGLWTLYAKWTAILPPPPPAGGVATGGGTGYLCDPAGDAELATAGAYDGYLYAAEAF
                     GGGQASAVRGTLSLKVTGLAGKLTAKAVLQGGSVSFKGTAWSAPGTDGTQHTELTASG
                     GERLDLFVRQNRIWGTLSGGKAGAAGLTLDGARNRFAESGDTAAAALLDAFKGYYTVA
                     LPAAEGGTLSASDAVDAAPQGAGYLTLTVGAKGSAKVAGVLADGTKVTQAGRLLLFDG
                     CGPAACVPFFAPLYAKKGWAGGLLWIDPETRTVETDRDIGWFIRWENPGRGGPDGFSL
                     LLDACGGFYGTGASLASAYLFGAETEGTVYFDALGEPRDWSVLPETVPVTAAGASLSV
                     AKGAKPKKVSEEGDVSYEYDEVNPANVTLTFASRTGLFKGKFNLYCDYEDATGKVVHK
                     AVSVPYAGVLAPVRGGAFDGLPAGLGHCLIPDSDPAVKAYRLKRSRPVWLEEQ"
     gene            complement(3974..6163)
                     /locus_tag="PLZ74_06525"
     CDS             complement(3974..6163)
                     /locus_tag="PLZ74_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOR98011.1"
                     /translation="MTWMPRVRCFLGGLLFAIEGVAAGDAALRIVRFEPTANVCRAGR
                     PETVHAVIRNAGAATAGGTLRLGMTEGVTVTGPQTSGMLELAPGAQTNVAWTVTAAAA
                     CDSVLSVSLEDGGRAWANSDIRVRFLPPMARRRLDYIPDPVPVKTEVLVGAHHCPLWE
                     ADKINLWANVLKHPERTPALGFYAQENPEVSDWETKWAVEHGISFFIYCWYRASQGGA
                     VKMRFGSAIHDALFKSRFADRMKFTIMWENQSKGIAGVADERDLFENLMPYWIENYFK
                     HPGYLKIGNRPLLFIYRPEFLVKDLGSVENVRAAFGKMRQACRDVGFDGLILLGEYRG
                     LDPKHLTRMKDLGLDYTFAYCWGIPGSPAPQQAIQAQMASIRKTQELGILPQVVTVSQ
                     AWSGWRDEGSIWKIPPTDFEELLREAKAFIATLPSDQLGSRMLLLDNWNEWGEGHYLA
                     PYREYGFGYLDAVRNVFAPDAGTHEDLLPEDIGLGPYDKAYRRHAAEQAVLNARLTRR
                     VTKPGADEPGLIGWWAFDEPEDDRVTLDYSGNGLGGNLENADRAPGIDGRALVCKGGS
                     AMIAPATALSPSNAFSVACWVKTDLAEQKHVWFINRIFGGSTSSGFRLGLQDGRPCFG
                     VPVTSFSHHLTGKEPLPVGRWVYLAGTFDGQTMRLYMDGEEQGTLTRPDPLQPNGQPL
                     CLGNYDAGHAAHFTGLLDEVKLYSRSLSAEEVRTHAVSAPAALGAHF"
     gene            6325..7677
                     /gene="glmM"
                     /locus_tag="PLZ74_06530"
     CDS             6325..7677
                     /gene="glmM"
                     /locus_tag="PLZ74_06530"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOR98012.1"
                     /translation="MQKLFGTDGIRGQANRFPVNAELALKLGKALGKQLRAQGYGSAR
                     AVIGKDTRLSGYMLETAITSGLVSAGCDVFLVGPVPTPAVAHLTRSLAADVGIMLTAS
                     HNPYDDNGIKIFSPLGYKLEDEAEEALTRLILETDLNTDDIRSDQLGKAYRLDDARGR
                     YIEFAKSTIDNAKLSGYKVVMDCANGAAYDIGPWIFRELGAAITKTGVAPDGLNINTE
                     CGAMHPERMAQLVKEQGADIGIALDGDADRVIFADANGSIVDGDRVLAACAIAFKKRG
                     LLARDTLVVTSMSNLGLHDAMRRHGIAVEVTDVGDRYVIDAMRKGGYALGGEKSGHLI
                     FHNHATTGDGIISALQVLRVMKQEGKTLAELADCMTEYPQRLVSLKVKEKRPVHEVPV
                     LAAAIQACEADLKEAGRVIVRYSGTEPKIRLLVEASESALVERWLERLTQAVAEGLGV
                     "
     gene            7908..8117
                     /locus_tag="PLZ74_06535"
     CDS             7908..8117
                     /locus_tag="PLZ74_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98013.1"
                     /translation="MSRITPRNFLTDNRLCENFRSGMSRPWTRASNMANGLLKKLALA
                     GIVGASAMFGAREAKATPIEWGFFI"
     gene            8095..8820
                     /locus_tag="PLZ74_06540"
     CDS             8095..8820
                     /locus_tag="PLZ74_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98014.1"
                     /translation="MNGGSLSDIFYDARVYPNGNAADYSANSITFRDQNGNIIANNPK
                     FSLIYFVNYAGGSSAIAGGLNGSGNSAFGWFNQDGQYDTGLTTGQTANYPNADNGYWR
                     MFVDLNGDGNYGTYDSSNGLFIGDPHERITGTSVANFQGFQTGVGTLSAGDISGWYTV
                     PEPSTGVLTAIGLSALALPNGTNVIHFRIAVTNLASFRLECRDSLTNAAWTSLGTFSA
                     TGAVTEVSDTNTAPARFYRAVSP"
     gene            8997..10202
                     /locus_tag="PLZ74_06545"
     CDS             8997..10202
                     /locus_tag="PLZ74_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98015.1"
                     /translation="MMTSNLLKLQSRGVTFLAPESTHVAPDVNPDRIAPGTVIHPGCR
                     LSGAELSMGPGCVIGAEAPATVCDCQLGAGVHLAGGFFERSTFLDGFKAGSGAHVRPG
                     CLFEEGSSIAHSVGVKQTVFLPWVTAGSLINFCDVLMAGGTSRKDHSEIGSSYIHFNF
                     TPHQDKATASLVGDVPHGVLLNQPAIFLGGQGGLVGPCVIAYGTVIPAGQIWRGDITV
                     PGHLVAKPAFRAPIDMPYDARLYPSVKRVLRNNLRYIGNVAALDMWYRVVRARFMPTD
                     DPYTAACLAGARQRLAEILDERMARLDELARKVERSLACAPDAAAAGEHRAFVEGWPA
                     LKAALAAWLDARECAAVPETVQAAIAAWPSANYLDAVQSLPADAAQTLADWLEQTVSA
                     ASEPYRRCW"
BASE COUNT         1895 a         3030 c         3361 g         1999 t
ORIGIN      
        1 gaaagcacat ccccgctgac ctacttctgg tacgagggcg agggcgacga cggcgaggat
       61 ctggttcctg caggacaggg ggctgacttc accacaccgc cggttacggc tgcgtcgtcg
      121 tattgggtga gggttgttga tgccttgggg caatacacgg acagcagggc cgtgcgtgtg
      181 atgcccgcgt ttgaaatccg cactgcggcg gatcttcaaa aagtgggatc gggcttggac
      241 ggctggatgc cggatgccgc ttatttggtg atgaatgaca ttgacgcatc ggagacggcg
      301 ggctggaatg aaggggccgg ctttgagccc atcggctggc acgactcatt tgcaggaatc
      361 ttggatgggc aggggcatac ggtcagccgc ctcacgatca accggcccga cgacggggcg
      421 gtcggtctgt ttggcttttt ggagaacggg gcctttgtca agaatgtcaa gctgcgcgat
      481 tgcacggtga tcggcggaag cgaagtcggg tgtctggcgg ggaccctgta cgagagtacg
      541 ctccaatcgt gttcagtcgc cggggtggtt tcaggcgatg aagcagtcgg tggtctggtc
      601 ggatggtgcg gcgatagcac tgtgcaatcc tgctatgcca caggtgccgt gtacggtcaa
      661 tataatgccg gtggcctgat cggttcgctt tccgacgata gcgccgtaca atcctgctat
      721 gccacagggg ccgtgtacgg ttcctatgta accggcggtt tggtcggagg gtgcggctat
      781 agtactgtgc aatcctgcta tgccacaggg acggtggaag gtgacgaagg tgtcggtggc
      841 ctgatcggtt cgctttacga ctatagtgcc gtgcaatcct gctatgccac cggggccgca
      901 tccggtcaaa attttgtcgg aggattggcc ggatattgtg acttcgatgg tgtcgttagg
      961 acctgttacg cggcgggggc tgttgacggc atgtccggcg caggcggttt gatcggatat
     1021 gcggaagagg cgtttattat tgaagactca tattgggata cggaggctag cggcttggcg
     1081 ctttcggcgg gcggtgaagg ccgcacaacc gcgcacatgc agcagcaggg caccttcaca
     1141 gggtgggact tctccgcaga gtgggggatt gtcaacggtg tcagctatcc ctatttgctg
     1201 tccttcccgc atccgttttc gctaacggcc tcaacccggg gcgcaggcgg cgtgaccgcc
     1261 gcgcctgcgg ccgaccggca tgcgccgggg gcgattgtga cgctcacggc ggtcgcagct
     1321 cccggccaga atgagttcgt gaaatgggtg ggcgcggtgt cggatccgtt gtcccccgtc
     1381 atcacggtgc gcatggacgg acacaaatcc gtgctggcgg ttttccggac attcagaggg
     1441 ctctcgtcga tcgccgacgt gcagaagatc ggttctgatc ccgcttatcc gtccgacggg
     1501 cggtattggc tggagcaaga catcgacgcc acggaaaccg cgcagtggaa cgacgagggc
     1561 acggacgagt cactcatgga gggcttccgt ccgatcaatc ttttcgccgg attttttgac
     1621 gggaacggtc acaccgttca cggcctttcc atcaatcgag ccgaaagcgc agaaggaaat
     1681 ggcacggccc tgttttccgt gattgaacgc ggggccgttg tcgcaaacct gggccttacg
     1741 gattgcctga tttctggacg ctatgctatc ggtgctctgg catgtgaaaa ttatggggtg
     1801 atcgagcgct gttatgcctc aggtgccgta atggcaccgt tgagcggttc atatgcggtg
     1861 tatacaaatg cttatgatgg ctacatctgg accaattaca tcaacacttc agttggcgga
     1921 tttgtttgtt acaacggagg acgtttaagc gagtgttatg cggccgttgc ggtcgacccg
     1981 ctggcacatg acgccggtgg ttttgtcctc tatcgtgagg aaaatgagcg tgatgtcgta
     2041 gaacgttgtt tctgggatac cgatgtgagc ggcttggatc cggagcaaag cgccggcgga
     2101 acggcctgct cgacccttca gatgaaacag tctgcgacgt ttttggaatg ggactttacg
     2161 gaggtctgga agatcgaaga gggcatcggc tatccgtcat tgcgcagctt tgataccttt
     2221 gtcatcgggt tggatgcctc cggagccggg gatatgccca tgttgcgtct gatcgccgga
     2281 cgcccgttcg gcaatctgcc gtctccctcg cgttcgggtt atgctttcgg cggttggtgg
     2341 acggggccgg gcgggacagg cgagcaagtc ttgccgaccg acatcgtgcc tggcgctgac
     2401 ggtctttgga cgctgtacgc aaagtggacg gcgattctcc ctccgccgcc gcctgccgga
     2461 ggcgtggcga ccggaggcgg caccggctac ctgtgcgatc ccgcgggcga cgcggagctg
     2521 gcgacggccg gcgcgtatga cggctacctc tacgccgcgg aggcgttcgg cggcggacag
     2581 gcctcggcgg ttcgcggcac gctgtcgctg aaggtgaccg gcctggcagg caagctgacc
     2641 gccaaggcgg tgctgcaggg cggcagcgtg agcttcaaag gcacggcgtg gagcgcgccg
     2701 ggaaccgacg gcacgcagca tacggaactc accgcctccg gcggcgagcg gctcgacctc
     2761 tttgtccgcc agaaccggat ctggggaacg ctctcgggcg gcaaggccgg cgcggcgggg
     2821 ctcacgctgg acggcgcgcg caaccgcttc gcggagtcgg gcgacaccgc ggcggcggcg
     2881 ctgctggatg ccttcaaggg ctactacacc gtggcgctgc cggccgcaga gggcggcacg
     2941 ctctcggcct cggacgcggt ggacgccgcg ccgcagggcg cgggctacct gacgctgacg
     3001 gtcggcgcca agggctcggc caaagtcgcg ggcgtgctgg cggacggcac caaggtgacg
     3061 caggccggcc ggctgctcct cttcgacggc tgcgggccgg cggcgtgcgt gccgttcttc
     3121 gcgccgctct atgccaagaa ggggtgggcc ggcggcctgc tgtggatcga ccctgagacg
     3181 cgcacggtgg agaccgaccg cgacatcggc tggttcatcc gctgggagaa ccccggccgc
     3241 ggcggcccgg acggcttcag cctgctgctg gacgcctgcg gcggcttcta cggcacgggg
     3301 gcgtcgctgg cctcggccta cctgttcggc gcggagacag aaggcacggt gtactttgac
     3361 gccctgggcg agccgcggga ctggtcggtg ttgccggaga ctgtgccggt gacggcggcg
     3421 ggcgccagcc tgtcggtcgc gaagggcgcc aagcctaaga aggtcagcga ggagggcgat
     3481 gtctcgtacg agtacgatga ggtgaacccg gcgaacgtga cgctcacgtt cgcgtcgcgg
     3541 accggactct tcaaaggcaa gttcaacctc tactgcgact acgaagacgc caccgggaag
     3601 gtggtccaca aggcggtgag tgtgccctac gcgggcgtgc tggcgccggt gcggggcgga
     3661 gcgttcgacg gtctgcccgc cggcctgggc cactgcctga tcccggacag cgatccggcg
     3721 gtcaaggcgt accggctcaa gcgctcgcgt ccggtctggc tggaggagca gtgaataacg
     3781 aatggtgagt ggtgaaccgg caggggcgta ggcgggaggg acgagcgtct gctcgtccgg
     3841 gcggcagggg cgcaggcccc gggagggtgc gccgccgctg gttcagtgcg cgggggattt
     3901 ttaacgcaaa gacgcagagg cgggcaggat tgcgcaaggc agaatcgcgt cggggcagac
     3961 ggatgtgagc cggtcagaag tgtgccccga gggcggccgg ggcactcacg gcgtgtgttc
     4021 gcacctcttc cgccgacagc gaccggctgt atagcttcac ttcgtcgagc agacctgtga
     4081 aatgcgccgc gtggccggcg tcatagttgc ccaagcacag cggctggccg ttcggctgca
     4141 gcggatccgg ccgcgtcagc gtgccttgtt cctcgccatc catatagagg cgcatcgtct
     4201 gaccgtcgaa cgtccccgcc agatacaccc agcgaccgac cggcagcggc tcctttccag
     4261 tcaggtggtg gctgaacgac gtcaccggaa cgccgaagca ggggcgtccg tcctgcagtc
     4321 cgaggcggaa gcccgacgac gtgctgccgc cgaaaatgcg gttgatgaac cagacgtgct
     4381 tttgctccgc gagatccgtc ttcacccaac acgcgaccga aaaggcgttt gaaggcgaaa
     4441 gggccgtggc gggcgcgatc atcgcgctgc cgcctttgca gacgagcgcg cggccgtcga
     4501 tgcccggcgc gcggtcggcg ttctccagat tgccgcccaa cccattgcct gaataatcga
     4561 gcgtcacgcg gtcatcctcg ggttcgtcaa acgcccacca gccgatcagc cccggctcgt
     4621 ccgcgccggg tttcgtgacc cgacgcgtca ggcgcgcgtt cagcacggcc tgttccgccg
     4681 cgtgacgccg gtaggccttg tcgtacgggc ccagcccgat gtcttccggc agcaggtctt
     4741 cgtgcgtccc cgcgtccggc gcgaagacgt tgcgcactgc gtcgagatag ccgaacccgt
     4801 actcgcggta gggcgcaaga tagtggccct cgccccattc attccagttg tcgagcagca
     4861 gcatgcggct tccgagctga tcggacggca gcgtggcgat gaaggccttg gcctcgcgca
     4921 gtagctcttc gaagtcggtc ggcgggatct tccagatgct gccttcgtcg cgccagccgc
     4981 tccatgcctg cgacacggtc accacctgcg gcaggatgcc cagttcctgc gtcttgcgga
     5041 tggacgccat ctgggcctgg atcgcctgct gcggtgccgg gctgccaggg atgccccagc
     5101 agtaggcgaa ggtgtagtcg agccccaagt ctttcatccg cgtgagatgt ttcgggtcca
     5161 gcccgcggta ttcgccgagc aggatcaagc cgtcaaaacc cacatcgcgg cacgcctgcc
     5221 gcatcttgcc gaaggcggcg cggacgttct ccacgctgcc cagatccttg accaagaatt
     5281 ccgggcgata gatgaagagc agcggccggt tgccgatctt gagatagccg gggtgcttga
     5341 aatagttctc gatccagtac ggcatcaggt tttcgaaaag atcgcgctcg tcggccacgc
     5401 cggcgatgcc tttcgactgg ttctcccaca tgatggtgaa cttcatcctg tcggcgaagc
     5461 gcgacttgaa aagcgcatcg tggatcgctg agccgaagcg catcttgacc gctccgcctt
     5521 gactggcgcg gtaccagcag tagatgaaaa aggagatgcc gtgttcgacc gcccacttcg
     5581 tctcccagtc cgagacttcg gggttctcct gcgcatagaa acccagcgcg ggcgtgcgtt
     5641 cggggtgctt cagcacattg gcccagaggt tgattttgtc tgcctcccag agcggacagt
     5701 ggtgcgcgcc gaccagcacc tcggtcttga ccggcaccgg gtcgggaatg tagtcgagcc
     5761 gacgtctcgc catcgggggc agaaaacgca cccggatatc ggagttcgcc caagctcgcc
     5821 cgccgtcctc caacgagacc gacagcacgc tgtcgcaagc ggccgccgca gtcacagtcc
     5881 atgccacatt cgtttgagcg cccggcgcca gctcgagcat gccggatgtc tgcgggccgg
     5941 tgaccgtcac gccctcagtc atgccgagcc tcagcgtgcc gcctgccgtc gctgcacccg
     6001 cgttgcggat gaccgcgtgc accgtctcgg gccggcccgc gcggcacacg ttggccgtcg
     6061 gctcgaaacg tacaatccgc agcgcggcgt cgcccgcagc gaccccttca atcgcaaaca
     6121 gaagtccccc aagaaaacac cttacacgtg gcatccaagt catgtttccc ccttgtctca
     6181 tttgaagacc tccaaaataa catggattgt cattctggga aagctgtcat gaccccggct
     6241 gccagccgat taccggctgc gcgattaccg actgtgccta ttttgctctt gcaggggggc
     6301 aaacgttcca gtaaaaattt gggtatgcaa aagctttttg ggacagatgg tattcgtgga
     6361 caggcaaacc gcttccctgt caatgcggaa ttggccttga agctgggcaa ggcgctgggc
     6421 aaacagcttc gcgctcaggg ctacggcagt gcgcgcgcgg tgatcggcaa ggacacgcgc
     6481 ctctcgggct acatgctgga aaccgccatc accagcggcc tcgtctcggc cggctgcgac
     6541 gtgtttctgg tcgggccggt tcccacgccc gcagtcgcgc acctgacgcg ttcgctggcg
     6601 gcggatgtcg gcatcatgct gaccgcttcc cataatccgt acgacgacaa cggcatcaag
     6661 atcttcagcc cgctcggcta taagcttgag gatgaagctg aagaggccct gacccggctg
     6721 atcctcgaga ccgatctcaa caccgacgac atccgcagcg accagctcgg caaggcgtac
     6781 cgcctggacg acgcgcgcgg ccggtacatc gaattcgcca agagcacgat cgataatgcc
     6841 aagttgagcg gttataaggt agtcatggac tgcgccaacg gcgcggccta tgacatcggc
     6901 ccctggatct tccgcgagct gggcgccgcc atcaccaaga ccggcgttgc gccggacggg
     6961 ctgaatatca acaccgaatg cggcgcgatg catccggagc ggatggctca gcttgtgaag
     7021 gaacagggag cggacatcgg catcgcgctc gacggtgatg ccgaccgcgt gatctttgcg
     7081 gacgccaacg ggagtattgt ggacggcgac cgcgtgctgg cggcttgcgc catcgccttc
     7141 aagaagcgcg gcctgctggc ccgcgacacg ctggtcgtca ccagcatgag caacctcggc
     7201 ctgcacgatg ccatgcgccg gcacggtatc gcggtggagg tcaccgatgt cggcgaccgc
     7261 tatgtgatcg acgccatgcg caagggcggc tacgcgctgg gcggtgaaaa atccggccac
     7321 ctgatctttc acaaccatgc cacgaccggc gacggcatta tcagcgcgct gcaggtcctg
     7381 cgcgtgatga aacaggaagg caaaaccctg gccgagctgg ccgattgcat gaccgaatac
     7441 ccgcagcggc tggtcagcct gaaggtcaaa gagaagcgcc ccgtccatga ggtgcccgtg
     7501 ctggctgcgg ccatccaggc ctgcgaggcc gatctgaagg aggccggacg tgtgatcgtg
     7561 cgctactccg ggaccgagcc caagatccgc ctgctggtcg aggccagcga atcggcgctg
     7621 gtcgagcgct ggctcgagcg cctcacgcag gccgtggccg aagggctggg cgtgtgagca
     7681 gagaggggaa tgaacgccca acgctcaact ctcaacgctc aacgctcaag tggggaaggg
     7741 gaatgaacgc tcaactctca acgttcaact ctcaacgttc aagcgaggag aactgcgaat
     7801 gaattccaag tttattaggt ttttacttga gagttgaaag ttgagcgttg agagttgaaa
     7861 gttaaatcat cccatgaagt ccgtctgcca cgccaaggaa acacgccatg agccgtatca
     7921 cgccgagaaa cttcctgaca gacaataggt tatgcgaaaa cttccgatcc ggcatgtcac
     7981 gtccgtggac acgggcttca aatatggcaa atggattact aaaaaaactt gctttggcag
     8041 gaatagttgg agcaagtgct atgttcggtg ctagagaagc aaaagctaca ccgattgaat
     8101 gggggttctt tatctgatat tttttatgat gcacgtgttt atcctaatgg aaatgctgca
     8161 gattactctg cgaattctat aacttttaga gatcaaaatg gaaatattat agcaaacaat
     8221 cctaaattta gtttaattta ttttgttaac tatgcaggag gttcttctgc aatagctggt
     8281 gggctaaatg gttctggaaa ttctgctttc ggatggttta atcaagatgg gcagtatgat
     8341 actggtttga caactgggca aactgcaaat tatcctaatg ctgacaacgg ttattggagg
     8401 atgtttgttg acttaaacgg agatgggaat tatggaactt atgattcttc taatgggctt
     8461 ttcatcggtg atccacatga gagaattaca ggtacctctg ttgctaattt tcaaggattc
     8521 caaactggcg taggaacact ttcggcaggt gatatttctg gatggtatac tgtaccggaa
     8581 ccttctacgg gtgtcttaac agccattggc ttaagcgcac tcgctttgcc caacggcacg
     8641 aatgtcattc actttcggat cgcggtgacg aacctcgcct ctttccgtct ggagtgccgc
     8701 gacagcctga cgaacgcggc gtggaccagc ctcgggactt tttccgccac cggcgcggtc
     8761 acggaggttt cggacaccaa taccgcgccc gcgcgctttt accgggcggt gagcccgtga
     8821 ttcgagtaat gggacatgaa cgtcgaacgc agaacgcaga acttcgaact cagaactgga
     8881 gatcgggaga aaacttcaac ttcgaactca gaacagggaa caacttcggt gttgggcgtt
     8941 gggcgttcga cgttcggagt ttgaattgaa taagttgagc gctgaaagtt gaacaaatga
     9001 tgacatctaa ccttctgaag cttcagtccc gcggcgtgac tttcctcgcg cccgaaagca
     9061 ctcacgtcgc gcccgacgtc aatcccgacc gcatcgcgcc gggcacggtc atccatcccg
     9121 gctgccgcct gagcggcgcg gaactttcca tgggccctgg ctgcgtcatc ggcgcggagg
     9181 cccccgccac ggtgtgcgac tgccagctcg gcgccggcgt gcatctggcc ggcggcttct
     9241 ttgagcgctc gacctttctg gacggcttca aggcgggcag cggcgcgcac gtgcgtcccg
     9301 gatgcctgtt cgaggaaggc tcatcgatcg cgcacagcgt gggcgtcaag cagacggtct
     9361 ttctgccttg ggtcaccgcc ggcagcctga tcaacttctg cgatgtgctg atggctggcg
     9421 gcacgagccg caaggatcat tccgaaatcg gctcttccta catccatttc aacttcacgc
     9481 cgcatcagga caaagccacg gcctcgctgg tcggcgacgt gccgcacggc gttctgctca
     9541 atcagccggc gatctttctt ggcggacagg gcggactggt cgggccgtgc gtgatcgcct
     9601 acggcacggt gatcccggcc ggtcagatct ggcgcggcga catcacggtg ccgggtcatc
     9661 tggtcgccaa gccggctttc cgtgcgccga tcgatatgcc gtatgacgcg cgcctctatc
     9721 cttcggtcaa gcgcgtgctg cgcaacaacc tgcgctacat cggcaacgtc gccgcgctgg
     9781 acatgtggta tcgcgtggtg cgcgcgcggt tcatgccgac ggatgatcca tacaccgcgg
     9841 cttgccttgc cggcgcgcgt caacgtctgg ccgagattct tgacgaacgc atggcgcgtc
     9901 tagacgagct cgcgcgcaag gtcgagcgtt cgctcgcgtg cgcgccggat gcggcggcgg
     9961 ccggcgagca tcgcgcgttc gtcgagggct ggcccgcgct gaaagccgcc ttggccgcct
    10021 ggctcgacgc gcgcgaatgc gctgccgtgc ctgaaacggt ccaggccgcc atcgcggcgt
    10081 ggccgtccgc caactatctg gacgcggtgc agagcctgcc ggccgacgcc gcgcagacgt
    10141 tggccgactg gctcgagcag acggtcagcg cagcatcaga accgtaccgc cgatgctggt
    10201 gagcagcaag tcgtcgccct gctggagcag cttgaacttc tccgggaagc cgctgagcgc
    10261 gggcttgccg gtgatggcgc cggtc
//
LOCUS       DAOSRB010000095        10219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6385_length_10219_cov_7.847009, whole genome shotgun sequence.
ACCESSION   DAOSRB010000095 DAOSRB010000000
VERSION     DAOSRB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6385_length_10219_cov_7.847009"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1439
                     /locus_tag="PLZ74_06550"
     CDS             147..1439
                     /locus_tag="PLZ74_06550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 domain-containing metallophosphoesterase
                     family protein"
                     /protein_id="HOR98016.1"
                     /translation="MSLLHRRAFLKSSLLATLAAQTKGMAAESPQTAPNSPAVSPILT
                     PPSLQIPAPTSMGIVWGVTTRATGFVDWATRPDFSDARRVFAGSTGLRTLDDAALGVR
                     LTGLAPDSVYHYRTGTVPIDFRGPYKILPGEPVLSDAYSFRTPGMRSPSSFAVINDTH
                     ENHKAFALLAAKLEELNPAVTVWNGDLCDWLDQMPKMLQTLLNPGKSAFAVSRPFLFT
                     PGNHDYRGLLARELPRLLLTREPTERASAYWTLERNYAYRQGEIALIGLDTGEDKPDG
                     HPAWGQLAQFTPYRELQAKWLADTLERPEIKSAPYLVAFCHIPLFDDSPKANPGDRLD
                     GWASWQRHCHDLWSPLFERYGLQLVITAHTHRFRFDAPTAKRSWAHMVGGAGCDVPPK
                     GQLTVLEGRVIGDRLKLTAHNLTDGSTLGEHTFAPRQV"
     gene            complement(1430..1897)
                     /locus_tag="PLZ74_06555"
     CDS             complement(1430..1897)
                     /locus_tag="PLZ74_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOR98017.1"
                     /translation="METTERLKIYRHLFRIGVPLIDRQHEQYLEWLDQLFTLTEQPSV
                     SRAAVNEAVAGAVAYAIEHFDAEEALMRSVAYPGYEGHVRKHDEFRSETDRLCADDPK
                     AVSPEERLFYLTKWLVNWFCDQTQVYDKALAVFLERQRSTCNGGSVGSEASQT"
     gene            complement(1914..3083)
                     /locus_tag="PLZ74_06560"
     CDS             complement(1914..3083)
                     /locus_tag="PLZ74_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOR98018.1"
                     /translation="MKAREICKGVQWMGCVDWNRRLFDALIPLPDGTSYNAYLVRGSE
                     KTVLFDAVDPAVSHVLLAQLADVPRLDYLVAHHVEQDHSGAIPAVLAKYPEAIVLCTP
                     KAKPLLTDHLAIAPERIRTVEDNETLPLGGKTLRFIHTPWVHWPETMCTYLPEDRILF
                     SCDFFGSHLATSALCSGTDPYVCEAAKRYYAEIMMPFRSMIRNNMKKVRELDVGLIAP
                     SHGPLHDHPEIMLSAYEDWSSDRVANQVVIPYMSMHGSTEMMVDHLVAALTARGVGVQ
                     PFELSTTDTGKLAMSLVDAATLVVGTPTVHVGPHPHVFAAVHLANALRPKVKYAAIIG
                     SYGWGTKAVEQVSALIPNLKVEMLGTLMCKGLPQADTFAALDALAENIRAKHEAL"
     gene            3314..3634
                     /locus_tag="PLZ74_06565"
     CDS             3314..3634
                     /locus_tag="PLZ74_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HOR98019.1"
                     /translation="MKKLLALAVLCVSSAMLTGCVFPMKGATTAALTLEHTVSDPIVD
                     NSVRAIKRGESYGGGILFFSTGDNSIDTAMRNGGITKVHHVNYKVMNILYLYNETVTI
                     VYGE"
     gene            3747..5120
                     /gene="purB"
                     /locus_tag="PLZ74_06570"
     CDS             3747..5120
                     /gene="purB"
                     /locus_tag="PLZ74_06570"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOR98020.1"
                     /translation="MDPFDPLTALSPLDGRYASKLEKLRPIFSETGLIRRRVSVEIAW
                     LEALCDAPGIPEALPLSDGERAALREIADKFSLEDARRVKAIEAITNHDVKAVEYFIK
                     ERLAGTTLASRGEFVHFACTSEDINNLAHALMLRDGLRVLRTAQERLTDALAAFARET
                     RDAAMLARTHGQPASPTTLGKELAVFVLRLRRQATQLDAVILPAKLNGAVGNYNAHLA
                     AYPDVDWPALARSVIEGRFGLRQNPLTIQIEPHDGMAELFDALARWNTVLLDLNRDIW
                     AYISLGYLGQKTVKGEVGSSTMPHKVNPIDFENSEGNLGLANAVLEHMARKLPVSRLQ
                     RDLTDSTVLRNMGVGFGYALTAYQATLKGLGKITLNAERLAADLDEAWEVLAEPVQTV
                     MRKLGQTNPYEQLKELTRGKQISAESMRGFIETLDLPEEDKRRLLALTPATYTGIASK
                     LVDAIQI"
     gene            5164..5769
                     /locus_tag="PLZ74_06575"
     CDS             5164..5769
                     /locus_tag="PLZ74_06575"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOR98021.1"
                     /translation="MNMEFLPSVSPEVFTWVVVPLLIFVARILDVSIGTLRVIFVSKG
                     YRLYAPILGFFEVVIWLLAIRQIMNHVDNVLCYLAYGLGFAVGNYIGIGLDERLSLGT
                     VLVRIVPRYDTTELIDHLRRMGFGASLVDIEGMSGKLKMIFAIVKRKDLTALLAIIQE
                     HNPQAFVTIEDVKTAKEGYFRPPQAKRAAPLSWLNALCNRK"
     gene            complement(5793..7145)
                     /locus_tag="PLZ74_06580"
     CDS             complement(5793..7145)
                     /locus_tag="PLZ74_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98022.1"
                     /translation="MKLTRRSFSQTSLAAILAAQSAPYLARGQAARRFRVVLIGAGWW
                     GTNLLHTALDSGVCDLAALCDVDERMLALCGEALAKRGVSRPKGYKDFRECLATEKPD
                     IALIATPDHWHALPMIEACRQGAHVYVEKPICHTILEGRAMVAAAARYGRVVQVGTHR
                     RASPHNQSAREFIRSGKLGPIGFIRCFVTYSGGPEKPKRNIEPPRGLDWDFWCGPAPL
                     RPFCGDLKNPWSSGIHPRGFRHFMDYANGQLGDWGIHWLDQVLWITGLRYPNTCHSVG
                     GRPIRGPAILTQEEQTTDAPDMQVVTWAFADGPTVTWEHRLFAGNPHERGENVGVYFH
                     GQKGVLHLGWQQGWTFYPSNRKEPVLHEDAQLNEPDGQNLRELFADFLDAIRTGRKPL
                     CDIESGHQATTMCLLGNLAMKHGRSVSWDGAKETTGEAAADALLRRPYRGAWTYPVAP
                     "
     gene            complement(7295..8662)
                     /locus_tag="PLZ74_06585"
     CDS             complement(7295..8662)
                     /locus_tag="PLZ74_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98023.1"
                     /translation="MMRQMFTRREALRVMGLGGAAILAGCRSPGGSRAVKGGAARWFR
                     GNLHMHTYWSDGRAFPEQAIALYKQLGYDFISVSDHNVFAEDRNVWRSVEDKGKGWPP
                     VVTRTLYDAYVKEFGSEVETRQENGKTWVRLKTHDEMVRRFGEPGRFLLMPAVEITQH
                     LSDRGIQVHMNYVNLPDVIPSVKGGPLNRRVDAERTEVRNVIRTSTEQVAQLAAKEKR
                     PYMLMLNHPQWIYWDIMPQHLIDNPAVRFFEVCNGGSAFAPHPDAPGLTNDSFWDAVN
                     AFRARDGAPLLYGVGSDDTHYYLNRTPEQRLADAWVMVRSAALTPEALFGAMHAGDFY
                     TSTGVTLEDVDFRPANRTLRVRVRPEPGVTYRIRFITTRKGFDPSVRTVEAPAVKGRG
                     ARTLPIYSADIGQTVSQVEGTTASYRMAPDDLYVRAKIESSVPSGYKRHFHPDVQVAW
                     TQPYR"
     gene            complement(8896..9900)
                     /gene="rnhC"
                     /locus_tag="PLZ74_06590"
     CDS             complement(8896..9900)
                     /gene="rnhC"
                     /locus_tag="PLZ74_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOR98024.1"
                     /translation="MEKKNSFTFTLNEEQQQALQIMLRMGNYRPKTVPHTIIAVEAQD
                     CVVNLYKSGKCLIQGKGAEDFVLFFLEPNVLQCATVGYEEILNPDLVAPHMGIDESGK
                     GDFFGPLVVCAVFVDPDLAHAMQELGVKDCKQLTDKAVFFIGNKTRQLLGPKRFAMVS
                     IGPEAYNRLYAKIRNVNSLLAWAHARCIENLLEAVPECPRAVADQFGAKQVIERALMK
                     KGRKIKLEQRHKAESDIAVAAASVLAREAFLRGLRRLGETYGIQVHKGASEQVKTSAI
                     ELVKKEGPEVLLKTCKCHFKTTDQVLAACGSSRAALGPEGQVVSRGKDSYSARGSGST
                     "
     gene            complement(9911..>10219)
                     /locus_tag="PLZ74_06595"
     CDS             complement(9911..>10219)
                     /locus_tag="PLZ74_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98025.1"
                     /translation="QDRRPPFFAKNGVPPDLGGGNGGIARLVSWRNGHETHSETGMTA
                     RAKTNPANSHSPVLKTTLPITYIKVILISIPVRSAGGGLTSGTVIATNPPCEAKGILI
                     "
BASE COUNT         1937 a         3342 c         2993 g         1947 t
ORIGIN      
        1 cgttcggaag aagtgaatgc gaccacccca gtcgcattta ctttgccaag tgaggctgcc
       61 atgcgtgccc gaaaaagaac attgccgcag aagaacggat tggggtatgc ttcaaaacat
      121 caggtttcaa aaagggagga tgagggatga gcttactgca ccgacgcgcg tttctaaaaa
      181 gttccctgct ggcgacactc gccgcgcaga cgaaggggat ggctgccgaa tccccgcaga
      241 cagccccgaa cagccccgcc gtctccccga tcctgacccc gccgtcgctg cagattccgg
      301 caccgacttc gatgggcatc gtttggggtg tcaccacccg cgccacgggt tttgtggatt
      361 gggccacgcg ccctgatttc tcggatgcgc gccgtgtgtt cgcgggcagc accggattgc
      421 ggactctgga tgacgcggcg ctcggcgtcc ggttgacggg ccttgccccg gattcggtct
      481 accactaccg caccggaacc gtgcccatcg acttccgcgg accttacaag atcctgcccg
      541 gcgagccggt cctgagtgat gcctactctt tccggacgcc ggggatgcgg tcaccctcct
      601 cgtttgcagt catcaacgac acgcacgaga accacaaggc cttcgcgttg ctggccgcta
      661 aactcgaaga gttgaatccg gccgtgacgg tctggaacgg cgatctttgc gactggctgg
      721 atcagatgcc caagatgctc cagaccctgc tgaatcccgg caagagcgcg ttcgccgtca
      781 gccgcccctt cctgttcact ccggggaacc acgactaccg cggtttgctc gcgcgggagc
      841 tgccgcggct tctcctgaca cgcgagccca ccgaacgcgc gagcgcgtac tggactttgg
      901 aacgcaatta cgcctaccgc caaggcgaga tcgccctgat cggtctcgac accggcgagg
      961 acaaaccgga cggacacccc gcctgggggc agctcgccca gttcacgccc taccgcgagc
     1021 ttcaggccaa gtggctggcc gacacgctgg agcgtcccga aataaaaagc gcgccctatc
     1081 tggtggcctt ttgccacatc ccgctgttcg atgactcgcc gaaggccaac cccggcgacc
     1141 gccttgacgg ctgggcctcg tggcaacgcc actgccacga cctgtggtcc ccgctctttg
     1201 agcgatacgg cctccaactg gtgatcacgg cccacacgca ccggttccgc ttcgacgcgc
     1261 cgaccgccaa acgttcctgg gcgcatatgg tgggaggcgc gggttgcgat gtgccgccca
     1321 aaggacagct cacggtcctt gagggacgcg tcatcggaga tcgcctgaaa ctcaccgcgc
     1381 acaatctgac cgacggctcc acgctcgggg agcacacctt tgccccgcgt caggtctgag
     1441 acgcctccga tccgacggat ccgccgttgc aggttgaacg ctgccgttcg agaaacaccg
     1501 ccagggcctt atcatagacc tgcgtctgat cgcagaacca gttgaccaac catttggtca
     1561 gataaaacag ccgctcctcc ggggacacgg cctttggatc gtcggcgcag agccgatccg
     1621 tctcactgcg gaactcgtca tgtttgcgca catgcccctc gtaccccgga taggcgacgg
     1681 aacgcatgag ggcctcttcc gcatcgaaat gctcgatggc gtaagccacc gctccggcga
     1741 ccgcctcatt gaccgccgcc cggctgacgg agggctgttc ggtcagggtg aagagttggt
     1801 cgagccactc cagatactgc tcgtgctgcc tgtcgatcag cggcacaccg atacgaaaca
     1861 gatggcggta aatcttcagt cgctccgtcg tttccacacg cacccccttc ccctcacagc
     1921 gcctcatgtt ttgcccggat attctcggcg agcgcatcca gcgccgcaaa ggtgtcggcc
     1981 tgcggcagtc ctttgcacat cagcgtgccg agcatctcga ctttgaggtt ggggatcagg
     2041 gccgaaacct gctcgaccgc tttggtgccc catccgtaag aaccgatgat cgcagcgtat
     2101 ttgaccttgg gccgcagcgc gttggccaga tgcacggccg cgaacacatg cggatgcggt
     2161 ccgacatgca cggtcggtgt gccgaccacc agagtggcgg catccaccag agacatggcc
     2221 agtttaccgg tgtcggtggt ggagagttcg aaaggctgca cccccacccc ccgcgcggtc
     2281 agggccgcca caagatgatc caccatcatc tcggtactgc cgtgcatgga catgtagggg
     2341 atcaccacct gattcgcgac gcggtcggag ctccagtcct catacgccga caacatgatt
     2401 tccggatggt catgcagcgg tccgtggctg ggcgcgatca gccccacatc cagttcccgc
     2461 acttttttca tgttgttacg gatcatcgac cggaacggca tcatgatttc agcgtagtag
     2521 cgtttcgccg cctcgcagac atacgggtcc gtgccagagc agagcgccga ggtcgccaga
     2581 tgcgagccga agaaatcgca ggagaaaaga atccggtcct caggcagata ggtgcacatc
     2641 gtctccggcc agtggaccca cggggtatgg atgaaccgca acgtcttccc gcccagcggc
     2701 agggtttcgt tgtcctccac cgtccggatg cgctccggcg cgattgccag atgatccgtc
     2761 agcaacggct tggccttggg tgtgcagagc acgatagcct cggggtattt cgccagcacg
     2821 gcgggaatcg cgccggagtg gtcctgttca acgtggtgcg cgaccagata atccaggcgc
     2881 ggcacgtccg ccaactgcgc gaggagcaca tgcgacacgg ccgggtccac cgcatcgaac
     2941 agcacggtct tttcgctgcc gcggaccaga taggcattat agctggtgcc gtccggtaaa
     3001 gggatcagcg cgtcgaacag acgccgattc caatcgacac accccatcca ctgaacacct
     3061 ttacagattt ctctcgcctt cattgctctc tccctctcgg gcgcgacccg gccgcacaaa
     3121 cctcgccggg cgccgtccga atgaccttgt gcccgtgtat gatgatggat tcggttggca
     3181 ctgtcaatcc gcggtgcgtc ggaggtttct catcgacggc ttgcaattgg ggtcggattt
     3241 atgcaacgat tgaacggatg cgaaacacgg caaggctcgc cgtgtcgcac ttcatcaaaa
     3301 aggagttatg aacatgaaga aattactcgc ccttgctgtg ttgtgcgtca gctctgccat
     3361 gctgaccggc tgtgtgtttc ccatgaaggg tgccactacc gcagcgctca cgctggagca
     3421 caccgtttcc gacccgattg tggacaacag cgtccgcgcc atcaagcgcg gtgagtccta
     3481 cggcggcggc atcctgttct tcagcaccgg cgacaactcg atcgacacgg cgatgcggaa
     3541 cggagggatc accaaggtcc atcacgtgaa ctacaaagtg atgaacatcc tgtatctcta
     3601 caacgagacc gtcaccatcg tgtacggcga ataaccgtgc cgccgggcat caggacccct
     3661 ttcttcggaa accgggctcc tgatgcccgc ccgtatcgct atgtccgcgc cgccggattt
     3721 gcctgcggcg ttttggagtc ctgcgcatgg atccgtttga cccgttgacc gccctgtcgc
     3781 cgctggacgg gcgctacgcc tcgaaactgg agaagcttcg ccccatcttt tccgaaaccg
     3841 gcttgatccg ccgccgcgtc agcgtcgaga tcgcctggct ggaggcgctg tgcgatgccc
     3901 ccggcattcc ggaggcactc cccctgtccg acggggagcg cgccgcgtta cgcgaaatcg
     3961 cggacaagtt cagcttggag gacgcgcgcc gcgtcaaagc catcgaggcg atcaccaacc
     4021 atgacgtcaa agcggtcgag tacttcatca aggaacggct cgccggcacc acgctcgcgt
     4081 cacgcggcga gttcgtccat ttcgcctgca cctcggaaga catcaacaat ctcgcgcacg
     4141 cgctgatgct gcgggacggg ttgcgcgtgt tgcgcaccgc ccaagagcgc ctgaccgacg
     4201 cgctggccgc cttcgcccgg gaaacgcgcg atgccgccat gctggcgcgc acccacggcc
     4261 agcctgcctc gcccaccacg ctggggaaag aactcgcggt ctttgtcctc cgcctccgcc
     4321 gacaggccac gcaactcgac gcggtcatcc tgccggccaa actgaacggc gcggtcggaa
     4381 attataacgc ccaccttgcc gcttatccgg atgtggactg gcccgccctg gcccgctcgg
     4441 tcatcgaagg ccgcttcggt ctgcgccaga atccgctcac gatccagatc gagccgcatg
     4501 acggcatggc cgagttgttc gacgcgctcg cgcgctggaa caccgtgctg ctggatctca
     4561 accgcgacat ctgggcctac atctcgctcg gttacctcgg gcagaagacc gtgaagggag
     4621 aggtcggctc ttcaaccatg ccgcacaagg tcaaccccat cgatttcgag aacagcgagg
     4681 gcaacctcgg cctggccaac gccgtgctgg agcacatggc ccgcaaactg ccggtctcgc
     4741 gcttgcaacg cgatctgacc gactcgaccg tactgcgcaa tatgggcgtg ggcttcggtt
     4801 acgcgctgac ggcctatcag gccacgctca aagggctcgg caagatcacg ctcaacgccg
     4861 aacggctggc cgcggatttg gatgaggcct gggaagtgct ggccgagccg gtccagaccg
     4921 tcatgcgcaa actcggccag acgaacccct atgagcaact caaagaactg acgcgcggca
     4981 aacagatctc cgccgagtcg atgcgcgggt tcatcgaaac actcgacctc ccggaagagg
     5041 acaaacggcg tctgctcgcg ctcacgccag ccacctacac cggaatcgcg tcgaagctcg
     5101 tcgacgcgat ccagatctga tgcgcggggg caacaaagcg tcccgcggaa aacgtgcgaa
     5161 caaatgaata tggaatttct cccctccgtc agtccggaag ttttcacatg ggttgtggtg
     5221 ccgctgttga tttttgtggc gcgcatcctg gatgtcagta tcggaacctt gcgcgtgatc
     5281 ttcgtctcga agggctaccg cctctatgcg ccgattctcg gattcttcga ggtggtgatc
     5341 tggctgctgg cgatccgcca gatcatgaat catgtggaca acgtcctgtg ctatctcgcc
     5401 tatgggttgg ggttcgcggt cggcaactac atcgggattg gcctcgatga gcgcctgtcg
     5461 ctcggcacgg tgctggtccg catcgttccg cgctatgaca ccacggaact gatcgaccat
     5521 ctgcgccgca tgggtttcgg cgcctcgctg gtcgatatcg agggcatgtc cggtaaactc
     5581 aagatgatct tcgccatcgt gaagcgtaag gatctgaccg cactgctggc gatcatccaa
     5641 gagcacaatc ctcaggcgtt cgtcaccatc gaggacgtca aaaccgccaa agaaggctat
     5701 ttcagaccgc cgcaggcgaa acgcgccgcg ccgttgtcgt ggctaaacgc cttgtgcaac
     5761 cgcaagtgac cccgcagggt ctgctttaaa aatcaaggcg cgaccggata agtccacgcg
     5821 ccgcggtacg gccgccgcaa cagcgcatcg gccgctgcct cgccagtggt ttctttcgca
     5881 ccgtcccacg acacgctccg accgtgtttc atggcgaggt tgccgagcag gcacattgtt
     5941 gtggcctgat gtccgctctc gatatcgcag agcggtttgc gccccgtgcg gatggcatcc
     6001 agaaaatccg cgaacagctc gcggaggttc tgaccgtccg gctcgttgag ttgcgcgtcc
     6061 tcgtgcagca cgggctcttt gcggttcgac ggatagaagg tccagccctg ttgccatccg
     6121 agatgcaaga cccccttttg gccgtgaaag taaaccccca cattctcgcc ccgctcgtgc
     6181 ggattccccg cgaaaagacg atgctcccac gtcacggtcg gaccgtccgc gaaggcccag
     6241 gtcacgacct gcatgtcggg cgcgtccgtg gtctgctcct cttgggtcag aatcgcggga
     6301 ccgcgaatcg gacgcccgcc gaccgagtga caggtgttcg gatatcgcaa cccggtgatc
     6361 cagagcacct gatccagcca gtggatgccc caatcgccga gctggccgtt ggcgtaatcc
     6421 atgaaatgcc ggaacccgcg cgggtggata ccgctgctcc aagggttttt gaggtcgccg
     6481 cagaaagggc gtaacggcgc gggaccgcac cagaaatccc agtccaatcc ccggggcggc
     6541 tcgatgttgc gctttggctt ctcgggtccg ccggaatagg tgacgaaaca acggatgaat
     6601 ccgatcggcc cgagttttcc ggaacggatg aattcgcgcg cgctttggtt atgcggcgag
     6661 gcgcggcggt gggtgccgac ctgcacgacc cgcccgtacc gcgcggcggc ggcgaccatg
     6721 gcacggccct cgaggattgt gtggcagatc ggtttctcca catagacatg tgcgccttgg
     6781 cggcacgcct cgatcatggg cagcgcgtgc caatggtcgg gtgtggcaat cagggcgata
     6841 tccggtttct cggtcgccag acactcgcgg aaatccttat agcctttcgg acggctcacg
     6901 ccccgtttcg ccaacgcctc gccgcacagc gccagcatgc gctcatccac atcgcagagc
     6961 gcggcgagat cacaaacccc gctgtcgagt gccgtatgga gcagattcgt gccccaccat
     7021 cccgcgccga tcagcacaac gcgaaaacgc cgcgccgctt ggccccgcgc cagatacgga
     7081 gcgctctggg ccgccagaat cgcggccagc gaggtctgcg aaaaacttct gcgagtcagc
     7141 ttcatgacgg tcctcccccc tgtctactca caccccaaag tcgtgctgaa gatggcatgg
     7201 cacgcccagt tccgcaggca gatcccgaac ttccccaacg gtttcgcttc cggcgttaca
     7261 ctgacgcgcc agatacgcgc acaacgcctt ttctttagcg gtaaggctgc gtccacgcta
     7321 cctgcacatc ggggtggaaa tggcgcttgt acccactggg gacactggac tcgatcttgg
     7381 cgcggacgta cagatcgtcc ggggccatgc gatacgaagc cgtcgtgccc tcaacctgac
     7441 tgacggtttg cccgatatcg gcagagtata tcggcaacgt gcgcgcaccg cggcctttca
     7501 ctgcgggggc ctccacggtc cgcacactcg gatcgaaccc ctttcgggta gtgatgaaac
     7561 ggatgcggta ggtcacgccc ggctccggac gaacccgaac acgcaacgtg cggttggcag
     7621 ggcggaaatc cacatcttcc agcgtcacgc cggtggaggt gtaaaagtcg cccgcatgca
     7681 tcgcgccaaa gagcgcctcg ggtgtcagtg ccgcgctacg gaccataacc caggcgtccg
     7741 ccagacgctg ttccggcgta cggttcagat agtagtgggt atcgtcggaa cccacgccgt
     7801 aaagcagcgg tgcgccgtcg cgcgcccgga acgcgttgac cgcatcccag aacgagtcgt
     7861 tggtcaaccc cggcgcgtcc ggatgcggcg caaaggcgga accgccgttg cagacttcga
     7921 agaaacgcac cgccggattg tcgatcaggt gctggggcat gatatcccaa tagatccact
     7981 gcgggtgatt gagcatcaac atgtagggcc gcttctcttt tgcagccaac tgcgcgacct
     8041 gttccgtact cgtgcggatc acattccgca cctccgtccg ttccgcatcc acacgcctgt
     8101 tcagcggacc gcccttaacg ctggggatca cgtcgggaag gttcacgtaa ttcatatgca
     8161 cctggatccc gcggtctgac aggtgctgtg tgatctccac cgcaggcatc agcaggaacc
     8221 ggcccggctc cccgaaccgc cgcaccatct cgtcgtgggt cttgagccgt acccaggtct
     8281 tcccgttctc ctggcgcgtc tcgacctcac tcccgaactc ctttacgtac gcgtcataga
     8341 gagtccgggt taccaccggc ggccaccctt ttcccttgtc ctcgaccgaa cgccagacgt
     8401 tgcggtcctc cgcgaacaca ttgtggtcgc tgacgctgat gaaatcgtat ccgagctgct
     8461 tgtacagggc aatcgcctgt tccggaaagg cccggccgtc agaccaatag gtgtgcatgt
     8521 gcaggtttcc gcggaaccac cgcgccgcac cgcctttcac cgcgcggctg ccaccgggcg
     8581 aacggcaacc ggccagaatc gccgctcctc cgagccccat tacccgcaac gcctcccggc
     8641 gcgtgaacat ctgtcgcatc atgtcctcct cgaattaact cttacggggc gagtataacg
     8701 cagatcgccc ccccggacaa ggcggtcgtt gcgtcagggc tcggagagtt attcctcaac
     8761 cgtgccgtgg cgaatgcctt ggggcagctc agcagaaatc gcgctcagcg cataaccggg
     8821 catcaggctc aacaaaagaa gacggtgcct catttgacct ccttgaccat tcccttcaaa
     8881 ttggatagcg gaacattacg tcgagccgct gccgcgggcg gagtaggagt ccttgccgcg
     8941 cgaaaccacc tgaccctcgg gaccaagcgc ggcgcgcgaa gatccgcagg cagccagcac
     9001 ctggtccgtg gttttaaagt ggcatttaca ggttttcaga agcacctcgg ggccttcttt
     9061 cttcaccagc tcgatggcag aggtcttgac ctgctcggac gcgcccttgt gcacctgaat
     9121 gccgtacgtc tcccccaacc ttctgagtcc ccgcaaaaag gcctcgcgcg ccagcaccga
     9181 cgccgccgcg accgcaatat ccgactcggc cttgtggcgt tgctccagtt tgattttgcg
     9241 cccttttttc atcagcgcgc gctcgatcac ctgtttcgca ccgaactggt ccgccacagc
     9301 acgcggacac tccggcacgg cctccagcag gttttcgatg caacgcgcgt gagcccaagc
     9361 cagtagcgag ttgacgttgc ggatcttggc gtagagccgg ttgtaggcct cgggaccgat
     9421 cgataccatc gcgaaccgtt tcgggccgag cagctgccgc gttttgttcc cgatgaaaaa
     9481 gaccgccttg tccgtaagct gtttgcagtc tttgacgccg agttcctgca tggcgtgggc
     9541 gagatcggga tccacaaaca ccgcgcacac cacgagcgga ccgaagaagt cgcctttgcc
     9601 gctctcgtcg atgcccatat gcggggccac gaggtccgga ttcagaatct cctcataccc
     9661 cacggttgcg cactgcagca cgttgggctc cagaaaaaag aggacgaaat cctccgcgcc
     9721 cttcccctgt atcaggcact tgccgctttt atacagattc accacgcaat cctgcgcctc
     9781 gaccgcaatg atcgtgtgcg gcaccgtctt gggccggtag ttgcccatcc gaagcatgat
     9841 ctggagtgcc tgctgctgct cttcgttgag cgtgaacgtg aatgaattct ttttttccat
     9901 cgcaccgaca ttagatcaaa atcccctttg cctcacaagg tggattggtg gcgatgacgg
     9961 ttccggatgt tagcccgccg cccgcgcttc gcacgggaat ggaaatgagg ataactttga
    10021 tgtaagttat aggcaaggtg gttttcaaaa ccgggctgtg ggagttggcg gggttggttt
    10081 ttgcgcgggc ggtcatgccg gtctcgctgt gcgtctcgtg cccgttgcgc cacgacacga
    10141 gccgggcgat gccgccgttg cccccgccga ggtccggggg cacgccgttt ttcgcgaaga
    10201 acggcgggcg cctgtcctg
//
LOCUS       DAOSRB010000096        10036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6516_length_10036_cov_6.965635, whole genome shotgun sequence.
ACCESSION   DAOSRB010000096 DAOSRB010000000
VERSION     DAOSRB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6516_length_10036_cov_6.965635"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1667)
                     /locus_tag="PLZ74_06600"
     CDS             complement(189..1667)
                     /locus_tag="PLZ74_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOR98026.1"
                     /translation="MSTALRYDVIALRILSGRVLGVLLGVFALTETLWADFSPDRAVM
                     GEKYRLNWNAAVDAEIDARIEKHRKADCVVALDGVPDGTVVCVEQLTHDFKFGSHIFN
                     FDQLGRDDWNDMYKSTFTNLWNAATVAFYWKNYEPVEGAVRFADGPHDGAAFWNSKAT
                     LSPKEKRLAFNEWRRPAPDPIVNFCLRHGLSVHGHAMIYGAYTPGWATNIVDASRLDA
                     VYEHRIRTLCKHYGDRIDQWDVVNESVRANSMPESPDDEGKWRFRMPKCYTFKSFKTA
                     EEHLPRRVRLAINDAGVMVKPYVPFIRHLLGKGARIDIIGVQMHIFSRDDVLKVADGL
                     ECMPNLTSWDPVDQLATLTKLSRLGRPIHISEITIPAPDDTPEGWRIQAQLMYDNYRL
                     WFSWPDVCRITYWNLVDYSYDKESLASGLYTKDLRRKPSYEALDRLINREWKTRMSIV
                     VNNGRIAFRGFRGMYLLSWMDAEGTMRSKRINLKGAVERVEF"
     gene            2016..4001
                     /locus_tag="PLZ74_06605"
     CDS             2016..4001
                     /locus_tag="PLZ74_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98027.1"
                     /translation="MKTSRLITAGWLVFACVSAWAQVPKLYVKTAADGGNDVTGDGSW
                     DAPYATIQRAMDVAVKEQKNVLTTISVQPGVYPGSNYVNSTSYFVDVRAQKRVNGVDV
                     DNDNPRETVIDGSINRQFGFDLRGWKMSDDGTLYRQSVCGFTVTNGLWTYYWRGYELT
                     TSHMGGGGVTVYNGAMASNMIVCCCITEPDDRIPPGKTQGNQHGAVQINGNNSLFTHS
                     FVYNCTNHNTAASLTGEITGGGVYMYGGTIRDCTVSNCWLIADKDVNGNRRGGGIYLA
                     ENQNGLVDRCVVVDCGTSGTRSCEGGGICNMKPNVVTNSFIANCTANGNGGGICCSYG
                     GNVYDCTVSNCINTAGYGGNFGATSGSEQWLGTFRGCRFINCSGTSVYLGFGIMDGCL
                     IENNTGTPLQMTGKNTILRNSVIRNNTGKKAAFAHTTGADGTGTVERCWFVDNDCGNN
                     PVALRGQTYARWLILRDTLIAGNTCKRGVSITAKTSWSDVTDPWVRVENCTVAGNTCS
                     DVGIGVNAGSGGTSYPDEITITNCLVTANNGKQAFNDYLKVNGTVGNIAYCAADEGKG
                     THLPDGCHNVTGTITFREGTFIPTGSSVATERGFALPRMREAGASDMGDGTFTIMKSG
                     EWGVKIVPNNVKSRIIGVAPEIGACEYMPSGTTVMVN"
     gene            4060..4980
                     /locus_tag="PLZ74_06610"
     CDS             4060..4980
                     /locus_tag="PLZ74_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR98028.1"
                     /translation="MNRRAFLASAGALVAYPIWGKVPEDEKPLVRVGLVTDIHHAYLP
                     EAWFDNGRFYDRALPRLEEAIKVFNERRSDLVIELGDFKDIAKKKDPKPGEDDRDKET
                     VIKLTKEAEAVFAQFNGPRYHVFGNHDNDMLDKSEFTALCPNPGIPPEKTYYAFDQNG
                     VTFIILDGNFNLKGEDYTGSKINWDWRESLIPPTQIAWLKETLAAAKGHVVVCIHQRL
                     DSGARPLHRVANGAEVQKILADSQKVAAVFQGHDHIGGYMKERGVGYYTLRAIVTGPQ
                     RENNAYAEAAVYPSGAVVVTGFAQAVTVHI"
     gene            5009..5872
                     /locus_tag="PLZ74_06615"
     CDS             5009..5872
                     /locus_tag="PLZ74_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98029.1"
                     /translation="MKGKRNVLAAVALAAAFAFADDGYLVVGHGGLMEKGGDSPIAKE
                     LVARFSPKNGTAVAVDVVEVTNAVALKACAPKSYAGIILVPGFDTGKMKDHSIVNAEH
                     LAAMNALLDSVRAKAVVAVEEGGDFENYQRLRDAFAGRAAVITTRDGGARLAMSWGRF
                     APEGMRRIALALKHAADPAKRDGIHPLPGKVTMEESWMVLDFETPALQGQSLIVSDTL
                     FRKFYVRSAGKTNWTRIDNLYVEDNKVYLDCSKISNPGGVRLNWWHLSPFKNIDHLPT
                     PGFVLALDAGR"
     gene            5898..9749
                     /locus_tag="PLZ74_06620"
     CDS             5898..9749
                     /locus_tag="PLZ74_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98030.1"
                     /translation="MKPKSILLAVLISVCSAPCSVFAQGWNALLDWPVSTVTNKAYLT
                     SMTERPWWNDAWQKRVPLLVSGQADEKDDKVIVDAIVDLGEKVEPKEVRVVTPWETVV
                     PCVCEKAEGSKVRLLFKTTLRQHENKPFLVYFGNPAAQAEKISADVTLETDEHTFRIR
                     NGVLDVVFDRRHVTEGLIRSLRILGSNCRTVFFDRATGYAKAGFVFHPNATNSWDKGT
                     VTADNALVKSIRFECADSTLTFTLYAEQPRIDWSYEIHRGDEASIRMNWGLGTGSAFD
                     DFFYCGKNGKILTQRAGLDHVTDSMTNPEGRFEDWLGEGWYAIGERRMGDIAGLAFDV
                     TAAGRLGYDSYYGVSAGLKLRHKLERGEKAAGSGAVVATLGDVNDYRRICKRIAKPVI
                     VSVGAVQAKVAKPYRIPRLDRDFCFDHNVGYGYGGDSGTAEPLMKDPAWADRICERLR
                     SYGSTAVCLMGYPWWMMPIEDKALYDRLIAAQTSGAFEDWVPKRKSPSWEEMKGRGQE
                     ILAFTKAIHAKGMGVHTWCGFVPGWSSKANGHFMKEINDLSVDMMSLRVKCGQDCGYS
                     NAIEGEGVALPSDLVKKNGGSPNYWSWENPQEAFDKFDFQHGLMRDFYTKFKKLHPDI
                     PVFLWNSENGEYSREKFMSEDEGYFDTMVVEMLPHAGFPHTKHVSKRMRSHFNNRVGH
                     TVWHHYYIMNPKTTERIYQIEWPFVCGVNGFSQENLSYETKDLEMSEVTADFFRFAEY
                     TCLGDKVARMAPVKNVGVYRDPKMFRADVLKKRLDKPYGAGSQQDGRVRSFSEIKNFA
                     YDVIGPKFFTAKDLAQYRVVYVPEDEVLAEGEAKELLAYAEQGGNAVFEGELSDGAKK
                     VFGELEDAKVKTLGKGKVLWFKDVRTDRLAKRDAKAIAEMRKTIADLGGVEPFTLTGS
                     WMIDGILQAGPDGMFLGAYNDGRAEEKGIVTLSPTVAHVRTTPLYVLDVKTGIRRSVT
                     NGVFEIAVAPRQCGYYLIGDDAFTAVPVVKVAEWKGAGVGSFRPRPHVPPVIDITGFK
                     PIVAIEFVRANQKGKPIRIARSVEAKYDVRAFTPASYDVKDCKAALAEARFIHFIDTD
                     TKAMDLVFADCAEELKALLKRSGTILISRSETGPAAQKFFKEIEVFDPNPSAKAGVGD
                     TWATWCGPEDHPYLTSCHKSLGKEEEVWGTKFWLWRSLCNYFQYRRVFGAWDAAKQQV
                     LFRPKVDGEKYAGLVIQEKVLGTGRVIFNENQRCFTDWYESKQFGDNWLSYITGVPST
                     EHAIKATAYHGGLGEIVEPKW"
BASE COUNT         2167 a         2883 c         3049 g         1937 t
ORIGIN      
        1 atgaagtttc atgtgtttca gggtttcatc caaatcgggg tcgggatcgg gatcgggatc
       61 gggagcgatt gtttcaccac agaacacaac cgcctcacag tctcaccgcc tcacagtctt
      121 acagcctttg agattgcggg ctccgcccgc atctcaatac gtcaaaaccc aatgtttgcg
      181 gggctttgct agaattctac ccgttcaact gctcctttta ggttaatccg cttcgaacgc
      241 atcgtgccct ctgcgtccat ccaggacagg aggtacattc cgcgaaagcc tcgaaacgcg
      301 atgcggccgt tgtttaccac aatcgacatc cgagttttcc actcgcgatt gatcagccga
      361 tcaagcgctt cgtacgacgg ctttcgcctg agatctttcg tgtagagccc ggaggcgagc
      421 gattccttgt cataggaata atcaacaaga ttccaatagg taattcgaca aacatccggc
      481 cacgagaacc aaagccgata gttgtcatac atgagttgag cctgaatccg ccatccctcc
      541 ggcgtgtcat ccggcgccgg gatggtgatt tcggagatgt gaatcggacg gccgaggcgt
      601 gaaagcttgg tcagcgtcgc gagctggtcg actggatccc aggatgtcag atttggcata
      661 cactcgagtc cgtccgcgac cttaaggacg tcatctcgac tgaagatatg catctgcacg
      721 ccgatgatgt cgatgcgcgc tcccttaccg agcaaatgac ggataaacgg cacatacggc
      781 ttaaccataa cccccgcgtc gttgattgcg agccgtaccc gcctgggaag gtgttcttcg
      841 gcagtcttaa aggacttaaa cgtgtagcac tttggcatac ggaatctcca tttcccctcg
      901 tcatccggcg attccggcat cgagttggct ctcacggact cgtttacaac atcccactgg
      961 tcgatacggt cgccgtagtg tttgcacaag gtacggatcc gatgctcgta cactgcgtca
     1021 agacgcgacg cgtcgacaat gttcgtggcc caacccggcg tataagcccc gtaaatcatc
     1081 gcgtgtccgt ggacagacag tccatgtcgc aggcagaaat tcacaatcgg atctggggcc
     1141 ggccgcctcc attcattgaa agccaacctc ttctcctttg gcgaaagggt tgctttggaa
     1201 ttccagaacg ctgctccgtc gtgcggaccg tccgcaaaac gaaccgcccc ttcgactggc
     1261 tcgtaattct tccaataaaa cgcaaccgtc gcggcattcc agagattcgt aaacgtcgac
     1321 ttgtacatgt cattccaatc atctcgtccg agttgatcga aattaaaaat atgtgagccg
     1381 aacttgaaat cgtgcgtcaa ctgttcaacg caaacaacgg ttccgtccgg aacgccgtcc
     1441 agcgccacca cgcagtcggc ctttcggtgt ttctcaatcc gcgcatcgat ctcggcatca
     1501 accgccgcgt tccaattcaa ccggtacttc tcgcccatca ccgcccgatc cggggagaaa
     1561 tccgcccata gcgtttcagt tagagcaaaa actccgagca ggactccaag cactcttcca
     1621 gacaatatcc taagggcaat cacatcgtat cgtaatgcag tactcatttt cactaccgtc
     1681 ccataggata gaccggcacc ccaccgatcc ccaaggttgg cgcacattgt gcgggggggg
     1741 taaaagtcaa ccctaaaaat tctcaaaacg tgtcggcttc aggggcgttt gcctgtagcc
     1801 cacaggcatc atgaaaaaac gaatccccca attgcttggg ttcgcgcctc cgaaaccaac
     1861 gaagacagtg tacaccgtac tgcggcagcc cgtccagtgg gtcccccggg aggattgact
     1921 gttttcaccc tcaccgtata ataggtgctg atcacaggaa ccggtgtgtt gccggttcac
     1981 cctatgggat attggtgcgt tcataagggg gcgatatgaa aacgtcaaga ctcatcacgg
     2041 cgggttggtt ggtgttcgcg tgtgtaagcg catgggcgca ggtgccgaaa ctttacgtca
     2101 aaaccgcggc ggacggcggg aatgacgtga ccggcgacgg ctcctgggac gcgccgtacg
     2161 cgacgatcca acgggcaatg gatgtggcgg ttaaggaaca gaagaatgtt ttgacgacca
     2221 tttcagtcca gcctggcgtc tatccgggca gcaattatgt aaatagcacc tcttatttcg
     2281 ttgacgtgcg tgcgcagaag cgcgtgaacg gcgtcgacgt cgacaacgac aacccgcgcg
     2341 aaacggtgat cgacggatcg atcaaccgcc agttcggctt cgacctgcgc ggttggaaaa
     2401 tgtccgacga tgggaccctc taccggcaga gcgtttgcgg cttcacggtg acgaatggac
     2461 tttggaccta ctattggcgc ggctacgaat tgaccacatc tcacatgggc ggcggcggcg
     2521 tgacggtata taacggcgcg atggcgtcca acatgatcgt ctgctgctgc atcaccgaac
     2581 cggacgaccg tatcccgccc ggcaagacac aaggcaatca acatggcgcc gtgcagatca
     2641 acggcaataa ttcgctgttc acgcattcgt ttgtctacaa ctgcaccaat cacaataccg
     2701 cggcttcgct cacaggcgag attaccggcg gaggagttta tatgtacggc gggacgatcc
     2761 gcgactgtac ggtctcgaat tgctggctta tcgcggacaa ggacgtcaac gggaaccgcc
     2821 gcggaggcgg catctacttg gccgagaatc agaatggact ggtggaccgt tgcgttgtcg
     2881 ttgactgcgg taccagcgga acgaggagct gcgaaggcgg cggcatctgc aatatgaaac
     2941 cgaatgtcgt caccaactcc ttcatcgcca actgcacggc gaatggtaac ggcggcggga
     3001 tctgctgcag ttacggcggc aacgtctacg actgcacggt atcgaattgc atcaacaccg
     3061 ccggttacgg cggaaacttc ggcgcgacca gcggctcgga gcagtggctc ggcacgtttc
     3121 gcggctgccg cttcatcaat tgcagcggga catccgttta tctcggtttc ggcatcatgg
     3181 acggctgcct gatcgagaac aataccggca cacccctgca gatgaccggc aaaaacacga
     3241 tcctgcgcaa ttcggtcata cggaacaaca ccggcaaaaa agcggcgttt gcgcacacca
     3301 ccggggccga cggcaccggc acggtcgagc ggtgctggtt tgtggacaac gactgcggca
     3361 acaacccggt tgcattgcgt ggccagactt atgccaggtg gttgatccta cgcgacacgc
     3421 tcatcgcggg caatacctgc aaacgcggcg tttcgatcac agcaaaaacg tcctggtcgg
     3481 atgtcaccga cccgtgggtg cgcgtcgaga actgcacggt cgcgggcaac acctgcagcg
     3541 acgtgggcat cggcgtcaat gcgggcagcg gcggcacgag ctatcccgac gagatcacga
     3601 tcaccaactg tctcgtcacc gccaacaacg gcaagcaggc tttcaacgat tacctgaagg
     3661 tcaacggcac ggtcggcaac atcgcctact gcgcggcgga tgagggcaag ggaacgcatc
     3721 tgccggatgg ctgccacaac gttaccggca cgatcacgtt cagggaaggg accttcatcc
     3781 caaccggaag ttcggtggca acggaacgcg gtttcgctct gccgcggatg cgtgaggctg
     3841 gagcgagcga catgggtgac ggaacgttca cgattatgaa gagcggcgaa tggggcgtca
     3901 agatcgtgcc gaacaatgtc aagagccgca tcatcggggt tgcgccggaa atcggcgcat
     3961 gcgaatacat gccgtccggc acaacggtaa tggtaaatta aaatccatcc ttcgcagggg
     4021 aataagaaaa gaaagggatc gatgactgcg gcaaaaaaca tgaacaggcg cgcctttctc
     4081 gcgagcgcgg gggcgctcgt cgcgtatccg atctggggaa aggtgccgga agatgaaaag
     4141 ccgctcgtcc gtgtcgggct tgtgacggac atccatcacg cttatctgcc cgaggcgtgg
     4201 ttcgataacg gtcgctttta tgatcgtgcc ttgccgcgcc tcgaggaggc gataaaggtc
     4261 ttcaacgagc gccggagcga tctcgtgatc gaactcggcg acttcaagga cattgccaag
     4321 aagaaggatc cgaaaccggg cgaggatgac cgcgacaagg agaccgtgat caagctcacg
     4381 aaggaggccg aggctgtctt cgcgcagttc aacggtccgc gttaccacgt cttcgggaac
     4441 cacgacaacg acatgctcga caagtccgaa ttcactgcgc tctgtccgaa cccgggcatc
     4501 ccgccggaga agacgtacta cgcgtttgat caaaacggcg tcacgttcat catcttggac
     4561 ggcaacttca acctgaaggg cgaggactac acgggctcga agatcaactg ggactggcgc
     4621 gagtcgctca tcccgccgac ccagattgcc tggcttaagg agacgctcgc cgccgcgaag
     4681 ggccacgtcg ttgtctgcat ccatcagcgc ctcgactcgg gcgcgcgtcc gctccaccgc
     4741 gtcgccaacg gcgcggaggt gcagaagatc ctcgcggact cgcaaaaggt cgccgcggtc
     4801 tttcagggac atgaccacat cggcggctac atgaaggaac gcggcgtcgg gtactatacg
     4861 ctcagggcga tcgtcacggg cccgcagcgc gagaacaacg cctacgccga ggcggcggtc
     4921 tatccgtccg gcgccgtcgt cgtgacgggt ttcgcgcagg ccgtgacggt tcatatctga
     4981 gggattgggg agaaggggtc gggggattgt gaaggggaaa agaaatgttt tggcggcagt
     5041 ggcactggcg gccgctttcg cgtttgcgga cgacggctat ctcgtggtcg gtcacggcgg
     5101 cctgatggag aaaggcgggg attcacccat cgcgaaggaa ctcgtggcgc gtttctcgcc
     5161 caagaacggc acggccgtcg cggtcgatgt cgtcgaggtc acgaatgccg tcgcgctgaa
     5221 ggcctgtgcg ccgaagagct atgcgggcat cattctggtg ccgggcttcg acaccgggaa
     5281 gatgaaagac cattcgattg tcaacgcgga gcatctcgcg gcgatgaacg cgcttctcga
     5341 ctccgtccgc gcgaaggccg tcgtggcggt cgaagagggc ggcgatttcg agaactacca
     5401 gcgactgagg gacgccttcg ccggacgcgc ggccgtcatc acgacgcgcg acgggggggc
     5461 gcggcttgcg atgagttggg gacgtttcgc gccggagggc atgcgccgca tcgcgctcgc
     5521 gcttaagcac gcggccgatc ccgcgaagcg cgacgggata cacccgctcc ccggcaaggt
     5581 gacgatggag gagtcctgga tggtcctcga cttcgagacg cccgctctgc agggccagtc
     5641 gctcatcgtc agcgacacgc tcttccgcaa gttctacgtc cgctcggcgg gcaagacgaa
     5701 ctggacgcgc atcgacaacc tgtacgtcga ggacaacaag gtctatctcg actgctcaaa
     5761 gatcagcaat ccgggcggtg tgcgcctcaa ctggtggcat ctgagcccgt tcaagaatat
     5821 cgaccatctg cccacgccgg gcttcgtcct cgcgctggac gcgggaaggt aaggagcagg
     5881 aaaggaaaca ctcaacgatg aaaccaaagt ccattctgtt agccgtcctc atctccgttt
     5941 gcagcgcacc ctgctccgta tttgctcagg gatggaatgc cctgctggac tggccggtgt
     6001 cgacagtcac gaacaaggca tatctgacat cgatgacgga gcgcccttgg tggaatgatg
     6061 cctggcagaa acgtgtgccg ctgttggtgt cggggcaggc cgacgaaaag gatgacaaag
     6121 tcattgtcga cgccattgtt gacctcggcg agaaggtgga gccgaaggag gtgcgcgtgg
     6181 tgacaccgtg ggagacggtc gtgccgtgcg tgtgcgagaa agccgagggg tcgaaggtgc
     6241 ggctcctctt caagacgacg ctccggcagc atgagaacaa gccgtttctt gtgtatttcg
     6301 gcaaccctgc ggcacaggcg gagaagatct ccgcagacgt gacgctggag acggacgagc
     6361 acacgttccg aatccgcaac ggcgtcctgg atgtggtctt cgaccgcaga cacgtgacgg
     6421 agggcctcat ccgttcgctg cgcatcctcg gctcgaactg ccgcacggtg ttcttcgacc
     6481 gcgcgaccgg ctatgcgaag gccggcttcg tgttccatcc aaacgccacg aatagctggg
     6541 acaagggcac ggtgacggcc gacaatgccc tggtgaagtc aatccgcttc gaatgcgcgg
     6601 attcgaccct tacgttcacg ctctatgccg aacagccgcg catcgattgg tcgtacgaga
     6661 tccatcgcgg cgacgaggcg tccatccgca tgaactgggg ccttggcacg ggcagcgcct
     6721 tcgacgactt cttctactgc ggaaagaacg ggaagatcct cacccaacgg gcggggctcg
     6781 accatgtgac ggacagcatg acgaatcccg aggggcgctt cgaggactgg ctgggcgagg
     6841 gctggtatgc gatcggcgag cgccgcatgg gcgacatcgc cggtctggcc ttcgacgtca
     6901 cggccgcggg ccgtctcggc tacgacagct actacggcgt ttcggcgggg cttaagctcc
     6961 ggcacaagct cgaacgcggc gagaaggccg cggggtcggg agcggtcgtc gccacactcg
     7021 gcgacgtcaa cgactaccgt cgcatctgca agcgcatcgc gaagcctgtc atcgtcagcg
     7081 tcggcgcggt gcaggcgaaa gtggcgaagc cgtataggat cccgcgcctc gaccgtgact
     7141 tctgtttcga ccacaatgtc ggctacggct acggcgggga cagcggcacc gccgagccgc
     7201 tgatgaaaga tcccgcgtgg gcggacagga tctgcgagcg cctgcgcagt tacggctcga
     7261 cggccgtctg cctgatgggt tacccctggt ggatgatgcc gatcgaggac aaggcgctct
     7321 atgaccgcct gatcgccgcc cagacgagcg gcgcgttcga agactgggtg ccgaagcgca
     7381 agagtccgag ctgggaagag atgaagggac ggggacagga gatcctcgcg ttcacgaagg
     7441 cgatccatgc caagggtatg ggcgtccata cctggtgcgg attcgtcccc ggctggtcgt
     7501 caaaagccaa tggccatttc atgaaggaga tcaacgacct cagcgtcgac atgatgagcc
     7561 tgcgcgtcaa gtgcggacag gactgcggct attccaatgc gatcgagggc gagggcgtgg
     7621 cactgccgtc cgacctcgtt aagaagaacg gcggcagccc caactactgg agttgggaga
     7681 atccgcagga ggcgtttgac aagttcgact tccagcacgg gctcatgcgg gacttctata
     7741 ccaagttcaa gaaactccat ccggacatcc ccgtcttcct ctggaattcc gagaacggcg
     7801 agtattcgcg cgaaaagttt atgagcgagg atgagggcta cttcgatacg atggtcgtcg
     7861 aaatgctgcc ccatgccggt ttcccgcaca ccaaacacgt ctcgaagcgc atgcgctcgc
     7921 acttcaacaa ccgggtcggc catacggtct ggcaccacta ttacatcatg aacccgaaaa
     7981 cgaccgagcg catctaccag atcgagtggc cgttcgtctg cggcgtcaac ggcttctcgc
     8041 aggagaacct cagctatgag acgaaggatc tggaaatgag cgaggtcacg gcggacttct
     8101 tccgtttcgc cgagtacacc tgtctcggcg acaaggtcgc gcggatggca cctgtcaaga
     8161 acgtcggtgt ctaccgcgac ccgaagatgt tccgagccga tgtcctcaag aagcgtctgg
     8221 acaagcccta tggggcggga tcccagcagg acggacgcgt ccgctcgttc agcgagatca
     8281 agaacttcgc ctacgacgtg atcggaccga agttcttcac cgcgaaggat ctcgcgcaat
     8341 accgcgtcgt ctacgtgccg gaggacgagg ttcttgcgga aggcgaagcg aaggagcttc
     8401 tcgcctatgc cgagcagggt ggcaacgccg ttttcgaagg cgagctgagc gacggcgcca
     8461 aaaaggtgtt cggcgagctt gaggatgcca aggtaaagac gctcggtaag ggcaaggtcc
     8521 tctggttcaa ggatgtgcgc acggaccgcc tcgccaagcg tgacgccaaa gccatcgcgg
     8581 agatgcgcaa gaccatcgct gacctcggcg gggtggagcc gttcacactc accggttcat
     8641 ggatgattga cggcatcctg caggccggtc cggatggcat gttcctcggc gcgtacaacg
     8701 acggcagggc ggaagagaaa gggatcgtga ccctttcccc caccgtcgcg cacgtacgca
     8761 cgactcccct ttacgtgctc gacgtgaaaa ccggaatacg ccggtcggtg accaacggcg
     8821 tctttgaaat cgcggtcgca ccgcggcagt gcgggtacta cctcattggc gacgatgcct
     8881 tcacagcggt ccccgttgtg aaggtcgcgg aatggaaggg cgctggggtg ggctcgttcc
     8941 gtccgaggcc ccatgtgcct ccggtcatcg acatcaccgg cttcaagccc attgtggcga
     9001 tcgagttcgt ccgcgcaaac cagaagggca agccgatccg gatcgcgcgt tcggtcgagg
     9061 cgaaatacga tgtgcgcgct ttcacgcccg ccagctatga tgtcaaggac tgcaaggcgg
     9121 cgctcgccga agcgcggttt atccatttca tcgacacgga cacaaaggct atggaccttg
     9181 tctttgcgga ctgcgcggag gagctgaagg cgttgctgaa gcgcagcggg accattctga
     9241 tttcgcgctc tgagacgggg cctgcggcgc agaagttctt taaggaaatc gaggtctttg
     9301 acccgaatcc ttcggcaaaa gccggcgtcg gcgatacttg ggcgacctgg tgcggccccg
     9361 aggatcaccc ctacctcacc tcgtgccaca aatcgctcgg caaggaagag gaggtctggg
     9421 gcacaaaatt ctggctctgg aggagccttt gcaactactt ccagtacaga cgcgtcttcg
     9481 gcgcttggga tgccgccaag cagcaagttc ttttccgccc caaggtagac ggcgagaaat
     9541 acgcgggact ggtcattcaa gagaaggtcc tcggcacggg ccgcgtcatc ttcaacgaga
     9601 accagcgctg cttcaccgac tggtatgagt cgaagcagtt cggtgacaac tggctttcgt
     9661 acatcacggg tgtgccgtcg accgaacacg ccatcaaggc aacggcttac cacggtggcc
     9721 taggcgaaat cgttgagccg aaatggtgaa aacgattctc ttgggtttct ggtttcaggg
     9781 tttcagggtt tctggtttca ggggtttcgg gtttctgggt ttctggtttc agggtttctg
     9841 ggtttctggg tttcatccaa atcggggtcg gggtcgggat cgggatcgat tatttaacca
     9901 cgaacacacg ggatagcact gctcgggttt ttatccacag atttacacag attacgcaga
     9961 ttttttgggg catcgctcgg gttttttgga acgccagagg gaattcctga ttttcatgaa
    10021 gtttcatgtg tttcag
//
LOCUS       DAOSRB010000097        10016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6533_length_10016_cov_6.160827, whole genome shotgun sequence.
ACCESSION   DAOSRB010000097 DAOSRB010000000
VERSION     DAOSRB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6533_length_10016_cov_6.160827"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PLZ74_06625"
     CDS             <1..167
                     /locus_tag="PLZ74_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOR98031.1"
                     /translation="PGHHDNPFGIRLSALMISKRIPDSSVPMSLLADHPNVQFNYLRS
                     GIGEVAVEMH"
     gene            202..1353
                     /locus_tag="PLZ74_06630"
     CDS             202..1353
                     /locus_tag="PLZ74_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOR98032.1"
                     /translation="MRTLKTIAAGHVCLDIIPTFLRGAPTLEAVMQPGKLVDMGPAVL
                     STGGSVANTGMALKRLGLPVRLMGKVGRDAFGAAVCDVFRTHDETLVKTMIVDPAVAT
                     SYTVVINPPGIDRIFLHCPGANDTFGADDVIPQLLDGMDIFHFGYPPLMRAFYTKTDE
                     LMRLFRKVKECGLTTSLDMALPDPQSPAGQADWRAILKQALPFTDIFSPSLDEIVFML
                     HRERGLKVREQAAEGVPMGGLIEEDVRALADELIALGVAVVMLKLGNQGAYLRVTSDE
                     TRLATCGCFDTASRTAWGGVEFFAPCFDAKVAGTTGSGDCTVAGLLAAIAKGLTPAES
                     LRTAVAVGSASVEQPDATSGVPTWTALDARIRAGWGRETPKVAFQAKWV"
     gene            1494..2084
                     /locus_tag="PLZ74_06635"
     CDS             1494..2084
                     /locus_tag="PLZ74_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOR98033.1"
                     /translation="MRKCFAHTARHQEDWEPLKTHLLNVAARARQFASAFGAGVEGYI
                     AGLLHDLGKYGIRFQERLHGKGRHIDHWTAGAWTAMKQYGKAGYCLSLIAQGHHVGLQ
                     QDTKESFKKICSRAPSPDTTLSADSMNELLQLFAEDGLSWPTLQKSFYSRTGKRCSDM
                     LDVRMLFSALVDADYLETEKFINTVEVKPSRPTRGA"
     gene            complement(2213..3400)
                     /gene="eboE"
                     /locus_tag="PLZ74_06640"
     CDS             complement(2213..3400)
                     /gene="eboE"
                     /locus_tag="PLZ74_06640"
                     /inference="COORDINATES: protein motif:HMM:NF035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HOR98034.1"
                     /translation="MSISYCLNIHPGESLDAVRQAITRHACAVKARVSPAAPYPLGLR
                     LSAAAAGELSDPAALAAFTDLLGCNDLFVNGINGFPYGTFHNTAVKTAVYQPDWSTPE
                     RLTYTGRLATLLAALLPENQTGNISTVPIGHKTTAQPATSSLPVRQITIMAEFLHDLK
                     QRTGREVVLALEPEPDCLLENTQDVIVWFEDGLLTEGRRWLCASRRRTPEEAESLLRT
                     HVGLCLDTCHFAVEFEDPLAALARFESAGLRVARIQLSAAIRAVISDASLARLADFID
                     PVYLHQTKIQLPRGPLLSLPDLTNETLMTARRHMGRELRTHFHVPLFYAGDGVLDSTH
                     TDLTPEFFAHTVAQGYPLEIETYTFNVLPSALRPASVIDSLVAEHDWVSVRLHRHHQQ
                     ERL"
     gene            complement(3411..5582)
                     /locus_tag="PLZ74_06645"
     CDS             complement(3411..5582)
                     /locus_tag="PLZ74_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98035.1"
                     /translation="MMRTVSRMMLGMLAMITWAEAGTSKTFHVSPNGCDTASGSWWRP
                     FATFERAQQAVAEFKARHPAEAIERIEVRFRGGTYPLKQPIRIRPEHGGTATIPVIYR
                     SSGDAPPVFTGGRLITGWTVRADGAWTTHLPEVKSGSWDFAQLFVNGARRFRPRIPRK
                     GYFTTTNDFETVEAGVNGFYYKEDGLDPAWSNLQDIEFHTLHIWSASRVRLKSIDASR
                     KIVRFLKTRNYNQYWGSYKGPGWGENGCRFWAENVKEAFGVPGEWYLDRPTGTLSYMP
                     MPGERPDTARVEVPILPLLLIVEGDADRPVINTRIEGLVFKQTCWTTPESGNFIPQAE
                     VNIPAAAEFVNARNVTVSRCVFTQLAGYTFAFGPGSHSNTVDACIMSDLGAGGVKIGP
                     PYAGYSMFESMRNDFQSKNELADMDKTSAITISNCRIVGGGRIHPAAHGVWVGHASHN
                     RILNNEIGDLYYTSVSLGWTWGYAEPSRAHHNEVAFNHMHHIGQGVLSDMGGVYTLGV
                     SPGTTVHNNRIHDINAFTYGGWGLYTDQGSSYIHMYNNLVYNTKTGGFDQHFGRENIV
                     ENNIFANARKDQVQRSRDEEHISFFFRNNILYWETDGNLLGNNWKSGTRGMKDGKPTQ
                     HYELGPNLYWHTSGKQELFPGKKTLAQWQAETGQDAGSLVADPLFENPAAGDFRLKPG
                     SPATQVGFKPFDTNAAGPDKSARLPKFKLRPVPTMYEITTP"
     gene            5733..6425
                     /locus_tag="PLZ74_06650"
     CDS             5733..6425
                     /locus_tag="PLZ74_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98036.1"
                     /translation="MALNRVRDVDNRVAVRRVLMSVSDKSGLDVLVPGLLETCPNVVI
                     YSTGGTFAAVRDMLDPAVAARHLVAVSEYTGQPEMQGGLVKTLDFKIYLGLLSETYNE
                     AHRQDLSRVGAEPIDMVVVNLYPFSQTVARAGVTPEDARTNIDIGGPCMVRAAAKNYL
                     RVASVTDPADYAALLEELRRTGGTLGFGTRLRLMKKAFAHTAGYDQAIAAFFADVAEP
                     DARSAYEVRDRL"
     gene            6422..7294
                     /locus_tag="PLZ74_06655"
     CDS             6422..7294
                     /locus_tag="PLZ74_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOR98037.1"
                     /translation="MIDLHLHSTNSDGSDTPEELVISGCQAGLTALSLTDHDTLDGTE
                     EFLNACRANGMTGISGIEISAHLDEGPGELHILGYGLNPTHPLVVESLDRVLEGRAWR
                     NEQILEKLNGLGLELEWADVLAYADEHVVGRAHFAQALIDRSYVSSCSEAFERYLGKG
                     APAYADRYRLYPEEGIRMIREAGGVAVIAHPFSWELREAELETGLRELKAYGLTGMEV
                     LYSEYTREQTVTLMRLAKRLDLLPTGGSDYHGLAKSDIRLGTGFGSLHVPDAYLPPLL
                     NAIGSDSAWVVRPD"
     gene            7327..8199
                     /locus_tag="PLZ74_06660"
     CDS             7327..8199
                     /locus_tag="PLZ74_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOR98038.1"
                     /translation="MDWTETLAELIRRTTSDLPRDVEAALRTAEAAEPAHSQAARLLR
                     AIGENSRLARAQSTPLCQDTGTLTFFWRVPRESDTLALEEATREAVALATRNGWLRRN
                     TIDTLTGSSVDENVAEDCPVCHIEQADTETTEVWLLQKGGGCENMSTQFSLPDEALGA
                     GRDMEGVRRCLLQAVWQTQGFGCAPGILGVCVGGDRAEGYRVAKQQLLRPLDDEAGIP
                     ELAALERRVLTEANQLGIGPMGMGGRTTLLGVKITARTRLPASYFVTVAYLCWACRRR
                     GIRCGRGGEREWLG"
     gene            8196..8783
                     /locus_tag="PLZ74_06665"
     CDS             8196..8783
                     /locus_tag="PLZ74_06665"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOR98039.1"
                     /translation="MTIMTETILQTPLSEAAVRGLNAGDGVRVCGRVYTGRDRLHKFL
                     MEGGACPVSLRDGVLFHCGPVVVPENGGWRVVAAGPTTSIREEPYMATLIERFGVRAI
                     IGKGGMGDKTREACVRFGCVYLQAVGGAATLLAQRVSRVDGVSFLEEFGATEAMWHFE
                     VNGIETVVGMDARGRSLFDEVREASGGVLKKLLGM"
     gene            8916..9938
                     /gene="fmt"
                     /locus_tag="PLZ74_06670"
     CDS             8916..9938
                     /gene="fmt"
                     /locus_tag="PLZ74_06670"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOR98040.1"
                     /translation="MRIVFMGSSDASATALRGILRLPLLNVVGVVTQPDRPAGRRQRF
                     TPCPCKAYATRKGVGPIISPEKVNAPEVLDQIAQLRPDVIAVVAFGQFLGKRLLALPP
                     YGCVNGHFSLLPQYRGAAPVQAAIAAGDPVSGVTIMQMAEGMDDGDMLLKAIEPIHSD
                     DTAATLMDRLAILCAVTLAKALRLMQEGGLKREPQDHAAATYAPKIQKADGLIDWHAS
                     AVVIERRIRAYDPWPGAFTFLPERLNGKQGNKARLKVFQAEVWRDPVPHMQDYVPGQI
                     CEISPSGPIVVTGNMPLRLTGVQLDGSRRMDGKSFLNGHPMQPGDRFSAVAAMQPDPE
                     MSGGVQ"
BASE COUNT         1880 a         2699 c         3420 g         2017 t
ORIGIN      
        1 atcccggcca ccacgacaac ccgttcggca tccggctctc cgcgctgatg atctcaaagc
       61 gcatcccgga cagcagcgtg ccgatgtcgc ttctggcgga tcatcccaat gtccagttca
      121 actacctgcg cagcggtatc ggcgaagttg ccgtcgagat gcactgaccg atccgtgcgg
      181 gatccgaaac gaggagaaac gatgcggacg ttgaaaacca ttgcggccgg gcatgtatgc
      241 ctggatatca ttcccacctt tctacgtggt gcgccaacgc tcgaagcggt catgcagccc
      301 ggcaaactgg tcgacatggg tcctgcggtg ctctccacgg gcgggtcggt ggctaacacg
      361 gggatggcct taaaacgtct cggcctcccc gtgcgcctca tgggcaaagt cggccgggac
      421 gcctttggcg cggcggtctg cgatgttttc cggacgcatg acgagacact ggtgaaaacc
      481 atgatcgtcg atccggcggt cgcgacctcc tacacggtgg tgatcaatcc gccggggatc
      541 gaccgcatct tcctgcactg tccgggcgcg aacgacacct tcggtgcgga cgatgtgatc
      601 ccgcaacttc tcgacgggat ggatatcttc cacttcggct acccgcccct gatgcgggcg
      661 ttctacacca agacggacga actgatgcgc ctgttcagga aggtcaagga atgcggactg
      721 acgacctcgc tggatatggc gctgcccgac ccgcagtcgc ccgccgggca ggcggattgg
      781 cgcgcgattt tgaagcaggc gctgccgttt acggacatct tctcgccgag cctcgacgag
      841 atcgtcttca tgctgcaccg cgagcgcgga ttgaaagtgc gcgagcaggc ggctgagggc
      901 gtgccgatgg gcggactgat cgaagaggac gtgcgtgcgc tggctgacga actgatcgcg
      961 ctaggcgtgg cggtggtgat gctcaagctg ggcaatcagg gagcttatct gcgggtgacc
     1021 tcagacgaaa cgcggctggc gacatgcgga tgttttgaca ccgcatcccg tacggcctgg
     1081 gggggtgtgg agtttttcgc gccctgtttc gacgcgaagg tcgcgggcac gaccggttcc
     1141 ggcgactgca cggtggcggg attattggcg gcaatcgcca agggactcac cccggcggag
     1201 agtttgcgca cagccgtagc ggtgggttct gcaagcgtcg aacaaccgga cgccacgagc
     1261 ggcgtgccga cttggacggc gctcgacgcg cggatccgtg cagggtgggg gcgggaaacc
     1321 cccaaagtcg cctttcaggc aaaatgggtg taggggatgc gggacgatgg gggaggccac
     1381 ccgttgaacg caaaacaaac caaagatcga acattaaacg ctgaataaat ctcccggtcg
     1441 gtagtgccag tattgtgctg gctactgatc gggcgatttt aattaagggg gatatgagaa
     1501 aatgttttgc acacacggca cgacaccagg aagattggga gccgcttaaa acgcacctgc
     1561 tgaacgtggc cgctcgcgcc cgccaattcg cctctgcttt cggtgcaggc gtcgaggggt
     1621 acattgccgg cctgctccac gacttgggga agtacgggat acggtttcaa gaacgattac
     1681 atggtaaggg gcggcacatc gaccactgga ctgctggtgc gtggaccgca atgaagcagt
     1741 atggaaaagc aggctactgt ctttctttaa ttgcacaagg gcatcatgtc ggattacaac
     1801 aagacaccaa ggaatccttc aagaaaattt gcagccgcgc gccttccccc gatacaacac
     1861 tatctgcgga ctctatgaac gaattgttac aactgttcgc agaagacggg ttgtcatggc
     1921 ctaccttaca gaagtcgttt tacagtcgga cgggtaaacg ttgctccgac atgctcgacg
     1981 ttcggatgct gttttcggca cttgtcgacg cggattacct cgagaccgag aagtttatta
     2041 acacagtcga ggtcaagccg tcgcgcccca cgcgaggcgc gtgaattgaa acctcctcgc
     2101 cgtgacgccg cgtcggttgg ccgccgtcgc gccccacgcg gggcgcgtga attgaaatag
     2161 cggctgtgtg gggtgggggc gaggctgaga tgtcatgccc tatgcgacgc gactacagtc
     2221 tttcctgctg gtggtgtcga tgcaggcgta cactgaccca atcgtgttcg gcgacgaggc
     2281 tgtcgatcac cgaggcggga cggagcgcgg aaggcagcac attgaaggtg taggtctcga
     2341 tctccagcgg atagccttgc gcgaccgtat gcgcaaagaa ttcgggtgtg aggtcggtgt
     2401 gggtggagtc gaggacgccg tcgcctgcgt aaaacagcgg cacatggaag tgtgtccgga
     2461 gttcgcgccc catgtgtcgg cgggcagtca tcagcgtttc gttggtcagg tccggcaggg
     2521 aaaggagcgg gccgcgcggt agctggattt tggtttggtg cagatagacc ggatcaatga
     2581 agtcggcgag ccgcgcgagc gatgcatcgc tgatgacggc gcggatggcg gcgctgagtt
     2641 ggatacgggc gacacggagg cctgcgcttt cgaagcgggc aagcgcggcg agcgggtcct
     2701 cgaattcgac ggcgaaatgg caggtgtcga ggcagagccc gacatgggtg cgcagtaacg
     2761 attcggcctc ttccggcgta cggcgtctgc tggcgcaaag ccagcgccga ccttcggtca
     2821 gtagcccgtc ctcaaaccag acgatcacgt cctgcgtgtt ttccaacagg cagtcgggct
     2881 cgggctccag cgcgaggacg acctcgcggc cagtgcgctg cttgaggtcg tgcaggaact
     2941 ccgccattat cgtgatctgg cggacgggaa gggatgaggt tgcaggttgg gcggttgttt
     3001 tgtgcccgat gggaacagtc gagatgttgc cggtctggtt ttcaggcagt agcgcggcca
     3061 gaagggtggc gagacggcct gtgtaggtca accgctcggg agttgaccag tcgggctggt
     3121 agaccgctgt tttgacggcg gtgttgtgga acgtgccata ggggaagccg ttgatgccgt
     3181 tcacgaagag gtcgttacag ccgagcaggt cggtgaatgc agcgagcgcg gcgggatcgg
     3241 aaagctctcc ggcggctgcg gcggaaaggc gcagtccgag cggatagggc gcggcgggcg
     3301 agacgcgggc tttgacggcg cacgcgtggc gcgtgatcgc ctggcgcacg gcatccagac
     3361 tttcgccggg atgaatgttc aggcaatagg agatactcat gatgaccggt tcagggcgtc
     3421 gtgatttcgt acatggtggg gacggggcgg agcttgaatt tcgggaggcg ggctgacttg
     3481 tcgggtccgg ccgcgttggt gtcgaagggc ttgaagccga cttgggtggc aggcgaaccg
     3541 ggcttgaggc ggaagtcacc cgcggcgggg ttttcgaata gcgggtcggc aacgagcgag
     3601 cccgcgtcct gaccggtctc ggcctgccac tgggctagcg ttttcttgcc cgggaagagt
     3661 tcctgcttgc cggaggtgtg ccagtagagg ttcgggccga gttcgtagtg ctgggtgggc
     3721 ttgccgtcct tcatgccgcg agtgccgctt ttccagttgt tgcccaacag gtttccgtca
     3781 gtctcccagt agaggatgtt gttacggaag aagaacgaga tgtgttcctc gtcgcgtgag
     3841 cgctgcacct gatctttgcg cgcattggcg aagatgttgt tctcaacgat gttctcgcgg
     3901 ccaaagtgtt ggtcgaatcc gccggtcttg gtgttgtaca cgaggttgtt gtacatgtgg
     3961 atataactgg agccctgatc ggtatagagc ccccagccgc catacgtgaa cgcattgatg
     4021 tcgtggatgc ggttgttgtg gacggttgtg cccggcgaaa cgccgagggt gtagacgccg
     4081 cccatgtcgg aaaggacgcc ttggccgatg tggtgcatgt ggttgaaggc gacctcgttg
     4141 tggtgcgcgc gggacggctc cgcgtatccc cacgtccagc ccagcgagac ggaggtgtaa
     4201 tacaggtcgc cgatctcgtt gttcaggatg cggttgtggg aagcatggcc gacccagacg
     4261 ccgtgcgctg cgggatggat gcgtccgccg ccgacgatgc ggcagttcga gatcgtgatg
     4321 gccgaagtct tatccatgtc ggcgagttca ttcttgcttt ggaaatcgtt ccgcatgctc
     4381 tcgaacatgc tatagcccgc gtatggaggg ccgatcttca cgccgcccgc gccgaggtcg
     4441 gacatgatgc aggcatcgac cgtgttggag tgagagcccg ggccgaaggc gaaggtgtaa
     4501 ccggcgagtt gggtgaacac gcaacggctg actgtgacgt tccgggcatt gacgaactct
     4561 gcggccgcgg ggatgttgac ctcggcttgg gggatgaaat ttccggactc gggggtggtc
     4621 cagcacgtct gtttgaaaac cagcccctca atccgcgtgt tgatgacagg gcgatccgcg
     4681 tcgccctcga cgatcagaag cagcgggaga atgggcacct cgacacgggc cgtatcggga
     4741 cgttcgccgg gcatcggcat ataggagagc gttcccgtag gccggtcaag gtaccactcg
     4801 ccgggcacgc cgaaggcttc cttcacgttt tcggcccaga accggcagcc gttttctccc
     4861 cagccggggc ctttatagct tccccagtac tggttgtagt tgcgggtctt cagaaaccgc
     4921 acgatttttc ggctggcgtc gatggacttc agccgcacac gcgaggcgga ccagatgtgc
     4981 agggtgtgga actcgatgtc ctgcaggttg gaccatgcgg gatcaagccc gtcttccttg
     5041 taataaaagc cgttcacacc ggcctcgacc gtttcgaagt cattggtcgt ggtgaagtac
     5101 cctttgcgcg gaatgcgcgg ccggaatcgg cgcgcaccat tcacgaaaag ctgggcgaaa
     5161 tcccaggagc cggacttcac ttcgggcagg tgagtggtcc atgcgccgtc ggcgcggacg
     5221 gtccagcctg tgatcaagcg tccaccggtg aagacggggg gcgcatcacc gctggatcgg
     5281 taaatgaccg gaatcgtcgc ggtcccgccg tgttcgggac ggatgcggat cggttgcttg
     5341 agagggtatg tcccgccgcg gaaacggacc tcgatccgtt caatcgcttc agccgggtgc
     5401 cgtgccttga attcggccac cgcctgttgc gcgcgttcaa aggtggcgaa cggacgccac
     5461 cacgaaccgg acgccgtatc gcagccgttg ggcgagacgt gaaatgtttt ggacgtgccc
     5521 gcctcagccc acgttatcat ggccaacatc cccagcatca ttcttgaaac cgtacgcatc
     5581 ataaacctcc ccttttttca ctacgtaaca taacagaccc gcttatggag ggaaaagcct
     5641 tttgtcttcc cgtcattccg ctgttgccaa aagtcggcct tctttctatg ctacaggtgt
     5701 atggcagaca agcgttggga gaaggagagc gtatggcttt gaatcgagtg agggatgtgg
     5761 ataaccgggt tgcggtgcgt cgcgtgctga tgagtgtttc ggacaagagc ggcttggacg
     5821 ttttggttcc cggcttgctg gagacctgtc caaacgtcgt gatctactcc acgggcggca
     5881 cctttgcggc cgtacgggat atgcttgacc cggcagtggc cgcccggcat ctggtcgcgg
     5941 tttccgagta tacggggcaa ccggaaatgc agggcgggct ggtcaaaacg ctggacttca
     6001 aaatttacct cggcttgttg agcgagacgt acaacgaggc gcaccggcag gatctttcgc
     6061 gcgtcggtgc tgaaccgatc gacatggtgg ttgtgaatct ctatcctttc agccagaccg
     6121 tcgcgcgcgc cggggtgacc ccggaagacg cccgcacgaa catcgacatc ggcggcccct
     6181 gcatggtgcg ggctgcggcg aagaactatc tgcgtgtggc ctcggtgacc gatccggcgg
     6241 attatgcggc gttgctggag gaactccgcc ggacaggcgg cacactgggg ttcggcaccc
     6301 ggctgcgtct gatgaaaaaa gctttcgccc atacggcggg ctatgatcag gcgatcgcgg
     6361 cctttttcgc ggatgtcgcg gagccggatg cccgaagcgc gtatgaggta cgcgaccgat
     6421 tatgattgat ctgcatctcc attcaacgaa ttcggacggt tcggatacgc ctgaagaatt
     6481 ggtgatcagc ggttgtcagg ccgggctgac ggctctatcg ctgacagacc atgatacgct
     6541 ggacggcacg gaggagtttc tgaatgcctg ccgcgcgaac ggcatgaccg ggatatcagg
     6601 catcgaaatc agtgcgcatc tcgatgaggg gccgggcgaa ctgcatattc tgggatatgg
     6661 cctgaaccct actcatccgt tggtggtgga gagtttggat cgggtgttgg aaggccgcgc
     6721 ttggcgtaac gagcagattc tggaaaagct caatggactg gggctggaat tggagtgggc
     6781 ggacgtgctg gcctatgcgg acgaacatgt ggtggggcgc gcgcatttcg cgcaggccct
     6841 gatcgaccgc agttatgtct cttcctgttc cgaggcgttc gagcggtatc tcgggaaggg
     6901 cgcaccggcc tatgcggatc ggtaccgcct gtatcccgag gaggggatcc gcatgatccg
     6961 cgaagccgga ggggtggcgg tgattgcgca tcccttctcc tgggagttgc gcgaagcgga
     7021 gttggagacc ggtctgcgcg aattgaaggc gtacgggctg accggcatgg aggtgttgta
     7081 ctcagagtac acgcgcgaac agacggtcac gctgatgcgg ttggcgaagc gcctcgatct
     7141 gctgccgacc ggcggctctg attatcacgg gctggccaag tccgacatcc gtttgggaac
     7201 ggggttcgga tcgttgcatg tgccggatgc ctatctgccg ccattgctga acgcgatcgg
     7261 gtccgacagc gcctgggtgg tcaggccgga ttaaccctgc gcgacaccgc aaaaaaaagg
     7321 atgggtatgg attggacgga gaccctcgcg gaattgatcc gacgcacgac aagcgatctg
     7381 cctcgggatg tggaggctgc gttgcgtacc gccgaggcgg cggaacccgc gcactctcag
     7441 gcggcccggc tgttgcgggc gatcggagag aattcgcggt tggcgcgtgc gcagtccacc
     7501 ccgctctgtc aagacaccgg aaccctgact tttttctggc gggtgccgcg tgaaagcgat
     7561 accttggcac tggaagaggc cacgcgcgag gcagtggcgc tcgccacgcg caacggctgg
     7621 ttgcgacgta atacgatcga caccctgaca ggctcttcgg tggatgaaaa tgtcgcggaa
     7681 gactgtccgg tctgccacat tgagcaggcc gatacggaga cgacggaagt ctggttgttg
     7741 caaaaaggcg gcggctgcga aaacatgagc acgcaattct cgcttccgga cgaggcattg
     7801 ggggccggcc gcgacatgga aggggtgcgt cggtgtctgc ttcaggctgt gtggcagacg
     7861 caaggctttg gttgcgcccc gggcattctg ggcgtgtgcg ttggcgggga ccgtgcggaa
     7921 ggctaccggg tcgccaaaca gcagttgctg cgaccgttgg atgatgaggc cggaatcccc
     7981 gagttggccg cgctggaacg gcgtgtcctg acggaggcca atcaacttgg tatcgggcca
     8041 atgggtatgg gggggcggac cacattgctg ggcgtgaaaa ttacggcgcg gacgcgtctg
     8101 cccgcctcct atttcgtgac ggtggcctac ctctgctggg catgccgccg acgcggcata
     8161 cggtgcggcc gcggggggga acgggagtgg ctgggatgac gatcatgacg gagacgatat
     8221 tacagactcc gctttcggag gccgcggtgc gcggattaaa cgcaggtgac ggagtgcgtg
     8281 tctgcggacg ggtgtacacg gggcgggatc gtctgcacaa gtttcttatg gaaggtggcg
     8341 cttgtccggt atcgttgcgg gatggcgtcc tgttccattg cggccctgtg gtggtgccgg
     8401 aaaacggggg gtggcgggtg gtcgccgcag gtccgaccac ttccatacgc gaagagcctt
     8461 atatggccac gctgatcgaa cgcttcggtg tgcgcgcgat catcggcaaa ggcgggatgg
     8521 gcgacaagac ccgcgaggct tgcgttcggt tcggctgcgt gtaccttcag gcggtggggg
     8581 gggcggccac actgttggca caacgcgtca gccgtgtgga cggggtcagt tttctggaag
     8641 aattcggtgc cacggaagcg atgtggcatt tcgaggtaaa cggaatcgag acggtggtcg
     8701 gcatggatgc ccggggccgg agcctgttcg atgaagtgcg cgaggcctcc ggcggcgtgt
     8761 tgaaaaaact actcggcatg taaggcaaag cgcatcaaat gcgtaatcag gttgcggccc
     8821 gaagacgcgt ctttccacac cttgcttttt ttatgcgcca tacacccgat aatctgctaa
     8881 aatagttgaa ttctgcaagc tacaggaagc cgtgaatgcg tatcgtcttt atgggatcat
     8941 cagacgcctc cgccactgcg ctaaggggga ttttgaggtt accgctgctg aacgttgtcg
     9001 gcgtggtgac acagccggac cgtcccgcgg gacggcgtca gcgcttcaca ccctgcccct
     9061 gtaaagctta cgccacccgc aagggggtgg ggccgataat ctcgcccgaa aaggtgaacg
     9121 cgccggaggt gctcgaccag atcgcccaac tccgtccgga tgtcattgcc gtagtggcct
     9181 tcggtcagtt tttgggcaaa cgactgctgg cactgccgcc gtacggttgc gtgaacggtc
     9241 acttttctct gctgccccaa tatcgcgggg cagcccccgt gcaggccgcg atcgctgcgg
     9301 gggatcctgt ttccggtgtg accatcatgc agatggctga aggaatggat gacggcgaca
     9361 tgcttctcaa ggcgatcgaa ccgatccatt cggatgatac ggcggcgacg ctcatggatc
     9421 gactcgcgat tctgtgtgcg gtgacgttgg ccaaggcgct gcggctgatg caggaaggcg
     9481 ggttgaagcg cgagcctcag gaccatgcgg cagccacgta tgcgccgaaa atccaaaagg
     9541 cggacggact gatcgactgg catgcctccg ccgtggtgat cgaacggcgt atccgtgcgt
     9601 acgacccgtg gcccggagcc ttcacctttc tgccggaacg gctgaacggg aaacaaggga
     9661 acaaggctcg tttgaaagtg ttccaagccg aggtgtggcg ggacccggtt ccccatatgc
     9721 aggattacgt gccggggcag atttgcgaaa tatccccgtc cggaccgata gtggtcacgg
     9781 gcaatatgcc gttgcgtctc acgggggttc agttggacgg cagccgccgc atggatggca
     9841 agtccttttt gaacgggcat cctatgcagc ccggtgaccg gttttctgcg gttgccgcga
     9901 tgcagccaga tcccgaaatg tcaggaggtg tgcaatgaat gtgagaaaag cttggtggtg
     9961 gagtgtgctc ggccgtgcgg gagggctgat tgtattggga gtgggtttgg tctcgc
//
LOCUS       DAOSRB010000098         9978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6566_length_9978_cov_6.150257, whole genome shotgun sequence.
ACCESSION   DAOSRB010000098 DAOSRB010000000
VERSION     DAOSRB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6566_length_9978_cov_6.150257"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1464
                     /locus_tag="PLZ74_06675"
     CDS             163..1464
                     /locus_tag="PLZ74_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOR98041.1"
                     /translation="MPKFAYIAVDAAGKESRGTIEAPNQAQAVAKVRSQGLFPTAIGV
                     AGGSDAATARPAAAAKKSPAAKKSLAQTRIEIKLPKFLRGKVKQKDLTTFTRQLATLV
                     DAGLPLLRGLHVLQRQTPNAALKDALAGMCESVESGSTFSESLTNYPKIFDNLYVNMV
                     KAGEAGGVLEVVLNRLAEFAEKAAKIKNKVKSAMVYPVVVLVAAVGITGFLLVAVIPK
                     FKDIFNDLLEGKPLPAITQFVMDASNLVMNNGLAVVAAFAALFVLFKIFAKTRFGRYL
                     LDVSKINAPMFGSLVRRTAIARMTRTLGTLLASGVPVLQALNIVRDTTGNEVISRAMQ
                     NVHDAVKEGESMTNPLAACKVFPPMVISMVEVGEETGALPDMLTRIAETYDDEVDNAV
                     AGLTSVIEPIMIIILAVVVGTIVIAMFMPMIQIIGSLGAQA"
     gene            1551..2612
                     /locus_tag="PLZ74_06680"
     CDS             1551..2612
                     /locus_tag="PLZ74_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOR98042.1"
                     /translation="MRNARNSVRKIGVSRGFTLIEMMVVIAVIGILIGGVFKLFSTAG
                     TSSQRAETIARLERLQNAISGFYAEFGTYPPVPSHGSPDPYMEADEDGTYTGSAELNP
                     TNANRAARCQPVAFCYPNARSLDFYINERYQSEGAVSVNDAFGPDVRNRNDYEWDQIR
                     IFRFGLMSYLMPRVELMLNGSELDNLSENNENIPNAYFFDTRQWTHYNAGSKISGPSG
                     GWWNKLRESLAAQRDRENHAVARWLPNLEKSVCGGPGDLLGINTTEPDTGLSMVTVND
                     PDSGVAYKLMRVSVRDGWGEDLYYYSAAPYQSYRLWSAGPDKKTFPPWISLESLSADD
                     RRIVSEWLKDDIARFDRSK"
     gene            2730..3476
                     /locus_tag="PLZ74_06685"
     CDS             2730..3476
                     /locus_tag="PLZ74_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOR98043.1"
                     /translation="MSETFMWYWNNIRHPVADGHRLGYGGGAGGTPALPTGIRIKHAL
                     HTAPPVSGRLRSGFTLVEMLLVIGILGILATALVGSFSYVQKAARQSQAQSLASELAT
                     ALNIYLQQERSWGDVLPNKSEMDEEACWVLQEKKLFDVTTYKSGGAEINNDSLDRYGL
                     LDPYGRALLRRDPGINAGEVIKHRLQIRLDDDYDGYVEAPDGTKVRASVLVWSRGPDG
                     KDDAEKGGRRYPDDDRLSWNHGKARAEAGK"
     gene            3540..4103
                     /locus_tag="PLZ74_06690"
     CDS             3540..4103
                     /locus_tag="PLZ74_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOR98044.1"
                     /translation="MVKSQISNLKPRSTGFTILELLVVVSIMAVIATLATGAVVKAVK
                     QGRKKRVEATCRALEMALTNYRAQEGKWPFKLSDLKRQGTEGQSRNDKKYWAHGADNA
                     KVFKDLYQGAGQKTVYLDASAVMALLDGQRVQLRAALSAGRRDVPLIYADPNETSILR
                     YYCVEYRVETDTVAVHPHSDHSPCKDK"
     gene            4112..4990
                     /locus_tag="PLZ74_06695"
     CDS             4112..4990
                     /locus_tag="PLZ74_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR98045.1"
                     /translation="MAKSQISNLKSRGFRSAAGFSLLELLSVMAIVALLSTLAVTSYF
                     NAIRGMTRRSAVKHLANSLILARQRACLEGVRVSVMVYNEVAGYNNKGDPECVPSYVV
                     CKEAGRVTALSGTTLVDEFTPLDKMFGTATEDDTVGSSYLGSMRLYNLTAGKWWQVTP
                     TAVSHSFSGRRSSYVANRSYTIPAFGFKKNANVRNANDAMWEVGDVYGLEAAPPGSLP
                     KGFKFINLDGNSDSLPENLNKVELITFLPDGTAQKAILPDGTAQKAMTVTIRELLPPK
                     LRSEIRVQRDGSIQYK"
     gene            5032..5616
                     /locus_tag="PLZ74_06700"
     CDS             5032..5616
                     /locus_tag="PLZ74_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98046.1"
                     /translation="MRGRMTERCGWNRVERYAVAGGDARDPGSIRPFSVRAGFSLMEV
                     NMAIFVMAVGILSMVALYPLGLRESVQGQQDLTQSMFADYLLNQAVAVASMTNFPWSQ
                     WKSVPSIASLDQSGITASQTDNRPAFMRERMRQADDWPSDRFRIICCRGAGGSDRIMG
                     IMVRSTEIEGEDVRNYTNNPVYYAEAYFQGDVTK"
     gene            5655..6458
                     /locus_tag="PLZ74_06705"
     CDS             5655..6458
                     /locus_tag="PLZ74_06705"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR98047.1"
                     /translation="MGTMVNLQIPNLKSRGCRAGTVSENVWPLRAGTPLRGVRGRFGE
                     PSLPTINRSHAGFSMIEVLVAASILVVIVMMLGMLFQQTSLAWRSGSFRAEAYMQVRS
                     YIGAIQRDVSAAVDARLLPQQYVTEEQNFSGGRLVFYTLTGGVELDQNDNLLFNRSLK
                     KITYDTAGNRTEQSLKPDGSWGAVKKANVLTFAERQSSKNRPGVKPLQFTPHFGQSGD
                     GLDSQGLPLHVTFGAQARSTGNTLDIGAWSFGPDGRPNTTDDIKTWVEE"
     gene            6490..6942
                     /locus_tag="PLZ74_06710"
     CDS             6490..6942
                     /locus_tag="PLZ74_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR98048.1"
                     /translation="MRKPAETLRASRLPSLGFTLVEMLVVVGMLGVLMAVSFSGVGQA
                     RKQAKIAKANSETRELINAWLAYEAAHDDWPVEISGDEIIADQGALGELLGENEEQAV
                     YLNASLTGGIFLDPWGNPYRLRLLYETRQNPKTDEFPASVTFPNRHRR"
     gene            6999..9686
                     /locus_tag="PLZ74_06715"
     CDS             6999..9686
                     /locus_tag="PLZ74_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98049.1"
                     /translation="MNRLFRYGRNGVGQGGSALLIVLGFLSFMMISAVSFAIFMRIER
                     QASSNYRHAITARHMLNAGLVRAIDEVDRHLRDVKFPPWPGRVYPGGTNTLSATVQDT
                     RVLSLESLSFLPGIFVNDVRYQALTGDTRPIWRQLSMPINFNSGENAFGGAEVGRFAF
                     VCVNLSDMLNVNAVSAAQSGTNRINIGHLFRNDGAANTFDNNKQNLDRRYETLQDFYA
                     CMFERNDPTFGSPYHKWLDTGDDLDFGFVSHHVLITDGIVKPEPGNANAFAITENKQP
                     IKNLGSAIPSMDPVFQTALGNALVGGLRNQQVIMAPMIADYLDNDHIPKRFDIPTVEM
                     TPMVSEIRLLQGLTPIIKQELVPDTSPPETIYYLQMTTAPGPQARIIEVEVTWPFKHH
                     QERSAIPNYSLEVKAFMKIIPNDGTSLNSASFAGGVAGPQYIPLSQAQDVPIPNAWNT
                     DGGDPNACYKKCIVHLDITQQNLRKNICRSSTKNFQLLDPQFVVNQPFCVALVLFVSV
                     KQGDQYVDIVPHGMSNPNLREPYLESVNKLFFRTAGSHVVVAQGVDLSNGTLGRPLPF
                     VWNGLEVPDPRFNYRVANWVKSGGSMGAVNASTTALLGQDGRDGDIYLSVSDLGRMCS
                     PGELGFIVRPFPHRSGNQDAGVDFDTHTLTDTPDHAHFYRTIRLYDHGGTGENQRRDR
                     IFEYFTEGDPHAVSSEVRVNPLSDLPQVLVAALQNTPVDWFCANEKREGRDFPVFNDT
                     DLMSASTWRAFTNGWLQCVEKVRNNTTITTARDQHLSIHYGDQQYFDWYAGQNPRVIF
                     GQTLSDPLHEVDRKMLFSWSIANFSDRQQLFLYVLRAEATVPSFGGSDMGGVKSVAGG
                     RAVALVWRDPYPVGYPDNMVSDPANFHEHRVLFFKQLDN"
     gene            complement(9753..>9978)
                     /locus_tag="PLZ74_06720"
     CDS             complement(9753..>9978)
                     /locus_tag="PLZ74_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HOR98050.1"
                     /translation="TIWNIGMALSLIASGKAGTAISYGLGQGATLVAALWGILIWKEF
                     KGAPKVAHQLNAAMFILFVAGLALIIRAGA"
BASE COUNT         2086 a         2795 c         3097 g         2000 t
ORIGIN      
        1 aatggaaccc tgaaacgcat gaccttcgat cgcacccctg tcacgaaccg attacaatgc
       61 gcttctcttt tacgtttgcc gcccaagtgc agactatgat atctttctct acggattcgg
      121 aatgcatgtg tgcgaggggt ccaaactatc cggagttgcc gtatgcccaa atttgcgtat
      181 atcgctgtgg atgccgcagg caaggagagc cgcggcacga ttgaagcccc gaatcaggct
      241 caggccgtgg ctaaggtgcg tagccagggg ttgtttccca ctgccatcgg tgttgcggga
      301 ggatcggatg ccgcaacggc ccggcccgcg gcagcggcta aaaaaagtcc tgcggcgaag
      361 aaatccttgg ctcagacccg aatcgagatc aaactcccca agttcctgcg cggcaaggtg
      421 aagcagaagg acctgacgac gttcacgcgt cagctcgcca cgctggtgga cgcggggttg
      481 ccgctgctgc gcgggttgca tgtgctccag cgtcagacgc ccaacgcggc gctcaaggat
      541 gccctggccg ggatgtgtga atcggtcgag agcggcagta ccttctcgga gtcgctcacc
      601 aactatccca agatcttcga caatctgtat gtcaacatgg tcaaggccgg cgaggccggc
      661 ggcgttctgg aagtggtgct caaccgcttg gccgaattcg ctgaaaaggc ggccaaaatc
      721 aagaacaaag tcaaaagcgc gatggtttac cccgtcgtgg tccttgtggc tgctgtcggc
      781 atcaccgggt tcctgctggt cgcggtgatc ccgaagttta aggatatctt taatgatctg
      841 ctcgaaggga agccgttgcc ggccatcacg cagtttgtga tggacgccag taatctggtg
      901 atgaacaacg gcttggcggt ggtcgcggct ttcgcggccc tgttcgtgtt gtttaagatt
      961 ttcgccaaga cgcgcttcgg gcggtatctg ctcgatgtgt ccaagatcaa tgccccgatg
     1021 ttcggctcgc tggtgcgccg cacggccatc gcccgcatga cccgcacgct cggcaccctg
     1081 ctggcctccg gtgtgccggt gctgcaggcg ctcaacatcg tgcgcgatac cacaggcaac
     1141 gaggtgatct cccgcgccat gcagaacgtg catgacgcgg tcaaggaggg cgagagcatg
     1201 accaacccgc tcgcggcctg caaggtcttc ccccccatgg tgatctcgat ggtggaggtc
     1261 ggcgaggaga ccggtgccct gccggacatg ctgacgcgga tcgcggaaac ctatgacgat
     1321 gaggtggaca atgcggtcgc ggggctgacc tcggtgattg aaccgatcat gatcattatt
     1381 ttggcggtag tcgtcggtac gattgtgatc gccatgttca tgcccatgat ccagatcatc
     1441 ggcagcctcg gcgcacaggc gtaaggggca ggttataata ggttacgggt taatggctaa
     1501 ccggccgaca gggatggttc cctgaaccgt ccgcgggaga aagagttttt atgcggaatg
     1561 cgagaaacag tgtgcgaaag atcggcgtgt cccgcggatt cacgctcatc gagatgatgg
     1621 tggtgatcgc ggtgatcggc attctgatcg gcggggtgtt caagctgttc agcactgcgg
     1681 gcaccagttc gcagcgtgcg gagaccatcg cccgtctgga gcggctgcag aacgccattt
     1741 ccggattcta tgcggagttc ggcacttatc cgcccgtacc gtcccacggt tcgcccgacc
     1801 cctacatgga ggcggacgag gatggcacct acacgggaag tgctgagctc aacccgacga
     1861 acgccaaccg cgccgcgcgc tgccagccgg tcgccttctg ttatccgaat gcccgttccc
     1921 ttgattttta catcaacgaa cgctatcaga gcgagggcgc cgtgagcgtg aacgatgcct
     1981 ttggtccgga tgttcgcaac cggaacgatt acgagtggga tcagatcagg atcttccggt
     2041 tcggtctgat gtcctatctg atgccgcgtg tcgagctgat gctcaacggt tcggagctgg
     2101 ataacctcag cgagaacaac gagaacatcc ccaacgctta cttcttcgac acgaggcaat
     2161 ggacgcacta caacgcggga tcgaagatct cgggtcccag cggcgggtgg tggaataaac
     2221 tccgggagag tctggccgcg cagcgggacc gggagaacca tgcggtggcg cgctggctac
     2281 ccaatctgga aaagagcgtt tgcggtggtc ccggagacct tttggggatc aacaccacag
     2341 agcccgatac cgggctgagc atggtgaccg tgaacgaccc tgatagcgga gtcgcgtata
     2401 agctcatgcg tgtctccgtc cgggacggtt ggggggaaga cctgtattac tattctgcgg
     2461 ctccgtatca aagctaccgc ctctggtctg ccggtcccga caagaagacc ttccctccct
     2521 ggatctcgtt ggaatcgctc tcggcagatg atcggagaat cgtttcggag tggctgaagg
     2581 atgacatcgc ccggtttgac cggtcgaagt aggaggagtg tgtgagcatg ggggtgtggt
     2641 gtagcccaac cggcctgcgc caccccgcaa gctcacagac aacgggtgtg cgaggacttc
     2701 ttgggtcgcg ggcttcgtcc gcatgtaaga tgagtgagac gtttatgtgg tattggaaca
     2761 acattcgaca tccggtggcg gacggccacc gcttaggtta tggaggcggg gccggaggga
     2821 cgcccgcgct cccgactgga atccgaatca aacacgcgct tcacacggca ccccctgttt
     2881 ccggccgact ccgttcgggt ttcaccttgg tcgagatgtt gctggtgatc gggatcctcg
     2941 ggatccttgc caccgcgctg gtcggctcgt tctcctatgt gcagaaggcc gcgcgccaat
     3001 cgcaggccca gagtctggct tccgagttgg cgacggcgct caacatctac cttcagcagg
     3061 aacggagttg gggggatgtg ttgccgaaca aatcggaaat ggatgaggag gcttgctggg
     3121 tactgcagga gaagaagctc ttcgatgtga cgacctacaa gagcgggggc gcggagatca
     3181 ataacgacag cctcgaccgg tacggactgc ttgatcccta tggccgcgcc ctgttgcggc
     3241 gcgaccccgg tatcaatgcg ggggaggtga tcaagcaccg cctccagatc cgtctggatg
     3301 acgattacga cgggtatgtg gaggcccccg acggcacgaa ggtgcgcgct tcggtgctgg
     3361 tctggtcgcg cggccccgat ggcaaggacg acgccgaaaa aggcggcaga cgctatccgg
     3421 acgatgaccg cttgagttgg aaccacggca aggccagggc ggaagcgggc aagtagggaa
     3481 caggaacgcc ggacatcgaa cgacgaacaa tgaacgctga agttccagaa gaggatacga
     3541 tggtgaaatc tcaaatctca aatctcaaac cgcggtcgac cggattcacc attctcgaac
     3601 tgttggtcgt ggtttcgatc atggcggtca tcgccacgct ggcgaccggt gcggtggtca
     3661 aggctgtcaa acaggggcgc aagaagcggg tcgaggccac ctgccgtgcg cttgagatgg
     3721 ccctgaccaa ttatcgtgcg caggagggca agtggccgtt taaactgagc gatctgaagc
     3781 ggcagggtac ggaaggccag agccgcaacg acaagaaata ctgggcgcac ggcgcggaca
     3841 atgcgaaggt cttcaaagat ctgtatcagg gcgcgggtca gaagacggtc tatcttgatg
     3901 cgtcggcggt gatggcgctg cttgatggtc agcgggtgca gcttcgcgcc gcgcttagcg
     3961 cgggcagacg ggatgtgccg ttgatctacg ccgatcccaa tgagacctcc atccttcgct
     4021 attactgtgt ggagtaccgg gtcgagactg acaccgtcgc ggtgcatccg cactccgacc
     4081 actccccctg caaagacaag tgagggacgc gatggctaaa tctcaaatct caaatctcaa
     4141 atcccgtggt ttccggtccg ctgcggggtt ctcgttgctt gagctgctct cggtcatggc
     4201 gattgtagcc ctgctctcca ctctggcggt caccagttac ttcaacgcga tccgcggtat
     4261 gacgcggcgc agcgcggtca agcatctggc caacagtctg atcctcgcgc gtcagcgcgc
     4321 ctgcctcgaa ggggttcgcg tcagcgtgat ggtgtacaac gaagtcgccg gatacaacaa
     4381 taagggtgat ccggagtgcg tgccctctta cgtggtgtgc aaggaggcgg gccgtgtgac
     4441 cgctctcagc gggaccacgc tggttgatga gttcacgccg ctcgataaga tgttcggcac
     4501 cgcaacggag gatgacacgg tcggttcctc ttatctcgga agcatgcggc tgtacaacct
     4561 cacggcgggc aagtggtggc aggtcacgcc cacggcggta tcccacagct tttccgggcg
     4621 gaggtcgtcg tatgtcgcaa accgcagcta cacgatcccg gccttcggat tcaagaaaaa
     4681 cgccaatgtc cgcaacgcga acgatgcgat gtgggaggtc ggggatgtct acgggcttga
     4741 ggccgcgccg cccggctcgc tgcccaaagg gttcaagttc atcaatctgg acggcaattc
     4801 ggattccctt cccgaaaacc tgaacaaggt cgagctcatc acgttcctgc cggacggcac
     4861 agcgcagaag gccatcctgc cggacggcac agcgcagaag gccatgacgg tgacgatccg
     4921 cgaactgttg ccgccgaaac tgcggagcga gatccgggtg cagcgggacg gttcgatcca
     4981 gtacaagtag caggagaagg aaacccggat tgggcggaca aggagatgac gatgcgcgga
     5041 agaatgacag agagatgcgg gtggaaccgc gttgagcgtt acgccgttgc gggcggggac
     5101 gcccgcgatc ccggctcgat caggcctttc agcgtacggg ccggattctc gctcatggag
     5161 gtcaacatgg cgattttcgt catggcggtc ggcatcctga gcatggtcgc gctttatccc
     5221 ttgggtttgc gcgaaagcgt tcagggccaa caggacctga cgcagtcgat gttcgcggat
     5281 tacctgctga accaggcggt ggctgtggcg tccatgacga attttccgtg gtcgcagtgg
     5341 aagagtgtcc ccagcatcgc gtctctcgac cagagcggca tcacggcttc gcagacggat
     5401 aacagaccag cctttatgag agagcgcatg cggcaggccg atgactggcc ttccgaccgc
     5461 ttccggatca tctgctgccg tggcgcgggc ggctcggacc ggatcatggg aatcatggtc
     5521 cgctccaccg agatcgaagg cgaagacgtc cggaattaca ccaacaaccc ggtctattat
     5581 gccgaggcct atttccaagg tgacgtgacg aaatagggga gaagttagag gttataagga
     5641 acaggtaaca ggtaatgggc acgatggtca acttgcagat ccccaatctc aaatcccgcg
     5701 ggtgccgcgc tggaacggtt tcggaaaatg tgtggccgtt gcgggcaggg acgcctctac
     5761 ggggcgtccg cggacggttc ggggaaccgt ccctgccgac aatcaaccgt tcccatgccg
     5821 gtttctcgat gatcgaggtt ctggtcgcgg ccagcattct ggtggtgatc gtgatgatgc
     5881 tcgggatgct cttccagcag accagccttg cttggcgctc gggatcgttc cgggccgagg
     5941 cgtatatgca ggtgcgttcc tacatcggcg cgatccagcg cgacgtctcg gccgcggtgg
     6001 acgcgcggct cctgccgcag cagtatgtga cggaggagca gaacttttcg ggtggccgtc
     6061 tggtctttta caccctgacc ggcggcgtgg agctggatca gaacgacaac ctgcttttca
     6121 accgcagtct caaaaagatc acctatgaca ccgcgggcaa ccgcacggag cagtcgctga
     6181 agccggacgg ttcctggggt gccgtgaaaa aggccaacgt gctgaccttc gccgagcgcc
     6241 agagcagcaa aaaccggccg ggtgtgaagc cgctgcagtt caccccccat ttcggtcaga
     6301 gcggcgacgg gctggactcc cagggcctgc cgctccacgt caccttcggt gcgcaggcgc
     6361 gtagcaccgg caacacgctg gacatcggcg cctggtcctt cgggccggac ggccggccca
     6421 acacgacgga tgatatcaag acgtgggtgg aggagtgaga gggggggggt tatgcacgat
     6481 cggaacaaca tgcgaaaacc ggcggagacg cttcgcgcct cgcgcctccc gtctcttggg
     6541 ttcacattgg tcgaaatgct ggtggtggtc ggcatgctgg gggtgctcat ggcggtctcc
     6601 ttctccggtg tggggcaggc tcgaaaacag gcgaaaatcg ccaaagcgaa ctctgaaacg
     6661 cgcgagttga ttaacgcctg gctcgcctac gaggccgcgc atgacgactg gccggtggag
     6721 atttcgggag acgagatcat tgcggaccaa ggcgcactgg gtgaactact gggcgaaaat
     6781 gaggaacagg cggtatacct caacgccagc ctgaccggcg ggatatttct ggacccgtgg
     6841 ggcaacccgt accggttgcg gttgctgtat gaaacgcggc agaatccgaa aaccgatgaa
     6901 ttccccgcat ccgtgacgtt ccccaaccgg cataggcgat aggggaggga aggttaaagg
     6961 actttgtgtg ataaaatagc cacattacgg acaataccat gaacagactt tttcgatacg
     7021 gacgaaacgg ggtggggcag ggcggctccg cgttgctgat cgtgctcggt tttttgagtt
     7081 tcatgatgat cagcgcggtc tcgttcgcca tcttcatgcg gatcgaacgc caggcctcct
     7141 ccaactaccg tcacgcgatc accgcgcgcc acatgctcaa cgcgggactc gttcgtgcga
     7201 tcgatgaggt cgaccgccac ctgcgggacg tgaaattccc gccctggccg ggccgcgtct
     7261 atccgggcgg aaccaacacc ctctccgcca cggttcagga tacccgcgta ctttccctgg
     7321 agtccctcag ctttcttccc ggcatcttcg tgaatgacgt gcggtatcaa gcgttgaccg
     7381 gggatacgcg tccgatctgg cgccaactca gcatgcccat caacttcaac agcggcgaga
     7441 acgctttcgg aggcgcggaa gtcgggcgct tcgcttttgt gtgcgtcaac ctctccgaca
     7501 tgctgaacgt gaatgcggtc agtgccgcac agtccggcac caaccgcatc aatatcggcc
     7561 acctgttccg aaatgacggt gcggctaaca cgttcgacaa caacaaacaa aatctcgacc
     7621 ggcgctacga aaccctgcaa gatttctacg cctgcatgtt cgaacgcaac gatccgactt
     7681 tcggaagccc ctatcacaaa tggctggata ccggtgacga tctggacttc ggcttcgtaa
     7741 gccaccatgt gctgatcacc gacgggatcg tcaaacctga acccggcaac gcgaacgcgt
     7801 tcgccatcac ggaaaacaaa cagccgataa aaaatttagg gtcagcgatt ccgagcatgg
     7861 atccggtgtt ccaaaccgcc ttggggaatg ctctggtggg aggactgcgc aaccaacaag
     7921 tcattatggc gccgatgatc gccgattacc ttgacaacga ccacattccc aaacggtttg
     7981 acatcccgac ggttgagatg accccgatgg tcagcgagat ccggttgctg caaggattaa
     8041 ctccgataat caagcaagaa cttgttccgg acacaagtcc gccggagacg atctactatc
     8101 tgcaaatgac aacggcaccg ggacctcaag cgcgtatcat tgaggtcgag gtgacctggc
     8161 cgttcaagca tcatcaggag cgtagcgcaa taccgaatta ttcgcttgag gtgaaggctt
     8221 ttatgaaaat cataccgaat gacgggacgt cgttgaactc ggccagtttt gcgggaggtg
     8281 tggccgggcc tcaatacata cccttgagtc aagcgcagga tgtgcccatt cctaacgcat
     8341 ggaacaccga tgggggtgac cccaacgcgt gttataaaaa atgcattgtg catttggaca
     8401 taacgcaaca gaatttgcgg aaaaacatct gccgatcctc cacaaaaaac ttccagttgc
     8461 tggatccgca atttgttgtg aatcagccct tttgtgtggc actggttttg tttgtctctg
     8521 tgaaacaggg cgatcagtat gtcgatatcg tgcctcatgg aatgtcgaat ccaaatctcc
     8581 gggagccgta tttggagtcc gtgaacaagc tctttttccg cacggcgggg tcgcatgtgg
     8641 tcgtggcgca gggtgttgat ttgtcgaacg gaactttggg caggcccttg ccttttgttt
     8701 ggaacgggct ggaagttccc gacccccgtt tcaactatcg tgttgccaac tgggtcaaga
     8761 gtggcgggtc catgggggcg gtcaatgctt cgactacggc gctgctgggc caggatggtc
     8821 gtgacgggga tatctatctc tcggtttcgg atttgggcag aatgtgttct ccgggtgaac
     8881 tgggttttat tgtcaggcct ttcccgcata gaagcgggaa tcaggacgcg ggagtcgatt
     8941 tcgatacgca cacacttacg gatacgccgg accacgcgca tttttaccgc acgatccggc
     9001 tgtatgacca tggcggaaca ggggaaaatc agaggcgcga ccggattttc gaatatttca
     9061 ctgaagggga tccgcatgcc gtatcatcgg aagtgcgggt gaatccgctc tcggatctgc
     9121 cgcaggtgct ggtggccgcg cttcaaaaca cgccggtgga ttggttctgt gccaacgaaa
     9181 agcgggaagg ccgggatttt ccggtgttca acgacacgga cttgatgtcc gcttctactt
     9241 ggcgggcgtt cacgaacggg tggttgcaat gtgtcgaaaa ggtccggaat aatacgacga
     9301 tcaccacggc gcgggatcag cacctctcca tccactacgg ggatcaacag tatttcgact
     9361 ggtacgcggg ccagaaccct agggtgattt tcggccagac gctctcggat ccgctgcacg
     9421 aggtggaccg caagatgctg ttttcttggt cgatcgccaa cttctcggac cggcagcagc
     9481 ttttcctcta cgtgctacgg gccgaggcca cggtgccctc gttcggtggc agcgatatgg
     9541 gcggggttaa gtccgtggca ggcggccgcg cggtggcgct ggtctggcgt gatccctatc
     9601 ccgtgggata tcccgataac atggtgagtg accccgcgaa tttccacgag caccgtgtcc
     9661 tgttcttcaa gcaactcgac aactgatgga atccctttca cagggggagc ggacttctca
     9721 aacagaaccg ttcatccccc gtgcgtggag gatcatgcgc cggcccggat aatcagcgcc
     9781 aaccccgcca caaacaggat aaacatggcg gcattcaatt ggtgcgcaac tttcggcgca
     9841 cccttaaact ctttccagat caggatgccc cacaaggcag ccaccagcgt ggcgccctga
     9901 ccgagaccgt acgaaatggc ggtgccggct ttgcccgacg cgatcaggct cagcgccatg
     9961 ccgatattcc agatcgtg
//
LOCUS       DAOSRB010000099         9954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6575_length_9954_cov_6.261137, whole genome shotgun sequence.
ACCESSION   DAOSRB010000099 DAOSRB010000000
VERSION     DAOSRB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6575_length_9954_cov_6.261137"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLZ74_06725"
     CDS             <1..310
                     /locus_tag="PLZ74_06725"
                     /inference="COORDINATES: protein motif:HMM:NF037850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98051.1"
                     /translation="VKVVLTPEKRRYGKLQVVMVTLADKDGNFVPDDDRRIHFQVEGC
                     RILAVGNSDPRGYDRFMKTDSHPLKFGRAGIFLEIEPGAKWKLTATAEGCVKAQKAAE
                     "
     gene            complement(437..1828)
                     /locus_tag="PLZ74_06730"
     CDS             complement(437..1828)
                     /locus_tag="PLZ74_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOR98052.1"
                     /translation="MNRNTLLLTGAALLALTGCDNPGTRCCPNTCGGDTVKLITLDPG
                     HFHAALVQKRMFPGVSPKVHVYAPAGPDLSLHLDRIRGFNTRAEDPTSWESVVYTGPD
                     FFDRMIAERAGNVVVLAGNNARKTEYILKSVQAGFHVLADKPMAITPKDYELLKQAFE
                     VARSKGVLLYDIMTERFEITSVLQRELSRFPTVYGEQEKGTPDDPAVTKESVHHFCKQ
                     VAGKPLQRPPWYYDTTQQGEAIVDVNTHLTDLIQWETFPGVALTPADIKVLKARVWPT
                     PITLEQFKTSTSQTAWPDYLKKDLDAQGVLQCRANGEFTYVIRGVHAKASVLWNFQPP
                     AGAGDTHYSLMRGTRSSLIIQQGEAEGYKPVLYVEPRRVAGVNKEELGRALTDAVATL
                     AKTYPGISAEPHETGWRIIVPEQYAVGHEAHFGQVMEAFLGFLQNRDMPDWEVPNMLV
                     KYHTLMEAYRLSR"
     gene            complement(1876..3288)
                     /locus_tag="PLZ74_06735"
     CDS             complement(1876..3288)
                     /locus_tag="PLZ74_06735"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98053.1"
                     /translation="MSTLTRRTFLRAGGATTAAITLPNLIPPHLFGAAAPSKQIHIGI
                     IGCGRIATSMEMPGLFGLTEMARITALCDLDSKRLAHSKARVENEYNKRLGLQNHTVK
                     TFGRYSDLLRDGGVDAVMICVPDHWHALCAIECALAGKDIWLQKPFSQTIHEGRILAE
                     IVRRKKVVLQVGSQQRSSKQFRTACELVRNGRIGRIRRVEVGFGKDRDGGRKEEMPIP
                     PNLDYDTWLGPTPEAYYTEDRVHNQDIAQIASRPGWIQMEPYGWGMITNWGAHHMDIV
                     QWGLGTEETGPLKVSGTCQWLDKGLWNAHREFDLLYTYPDDIKVSVCYRYPNGVRFIG
                     DEGWIFVSRGAAKVTASDPTTPGKTLKALDASDPRLLEAPLHANAVRLHVSDNHVKDW
                     LLAIQNRTSPVTNAEIGHRSTSVCTLGHLCMKTGKTLAWDWRTERTDDAGANALLACP
                     ERGPFSIPKVLQAADIRVSV"
     gene            3746..4561
                     /locus_tag="PLZ74_06740"
     CDS             3746..4561
                     /locus_tag="PLZ74_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOR98054.1"
                     /translation="MMETIGQKLLDALKLYGVKVLFALGILLVGWFVARMIRQALKNL
                     MRKAHLDDTLVTFLASLSHTAVMILVIITALGQVGLQTTSFVAVLGAAGLAVGLALQG
                     SLSNFAYGILMVLFKPFRAGDYIEGAGVGGLVKAVGIFTTELRSPDNKTIIVPNAKMM
                     GDNIVNYSANATRRLELKICVSFADDLKKAKQVLESVLEAEPRVLKDPAPFVGVFALS
                     DYGVEFTVRPWVKTPEYWDVRFALLEAIKTRLDAEGITIPLPQQVVHLHRNEE"
     gene            4648..5757
                     /locus_tag="PLZ74_06745"
     CDS             4648..5757
                     /locus_tag="PLZ74_06745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOR98055.1"
                     /translation="MKSRRFWHTAGVLIGVSAAVWVCAAAELSGGREDPDGKTVWYDG
                     QSLPIEGRAFQDTEVFYDRLPARAQKTVTPGVWGNSRHSAGMAFRLVTDAPTVKVRWG
                     VRNASLAMPHMPATGVSGVDVYRRLPEGGWYFVRNGRPGSFTNSLTSFSITPNTEFMI
                     YLPLYNGTSFLEVGIPKGNRLFAPPVRASGITKPVVFYGTSITHGGCASRPGLAFTAV
                     AGRFADVPVVNLGFSGSGKMEMALCDLIAEIDASVYVLDCLWNMGDALVKERAEPFIR
                     ALRERRPDTPILLAEDCNVFGRAPTSKSLLMRAIYDKLKAEDPARWAKLHYLEAKDML
                     GHDGEGTVDGCHPNDLGMMRQGLVYGQALKKALGR"
     gene            5787..6638
                     /gene="nifU"
                     /locus_tag="PLZ74_06750"
     CDS             5787..6638
                     /gene="nifU"
                     /locus_tag="PLZ74_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HOR98056.1"
                     /translation="MWEYSDKVMDHFRNPRNVGKIENPDGVGTVGSLACGDALTLMFR
                     LDADGRIAEVKFQTFGCASAIASSSALTEMILGKTLEEAERITNREIAEYLGGLPDQK
                     MHCSVMGREALEAAIRNYRTGETTVKKRDDPIVCTCFGVSEGEIRRVVTENGLTQVEE
                     VTNFCKAGGGCGMCRDDIQKTIDVLRAQPVGPSVATVAAPAKKLTNLQKMKRIEEVVN
                     REIRPQLQKDGGDIELIDIVGDCVTIAFRGMCAGCHSAAFTRQDFIEARLREFVDPAI
                     TVIEEEK"
     gene            6641..8002
                     /locus_tag="PLZ74_06755"
     CDS             6641..8002
                     /locus_tag="PLZ74_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOR98057.1"
                     /translation="MSLYEYAHDLDTPVHPVSAPDYTPVEELRALQLHRLQGLVRRVY
                     DHVPLTRARMDERGVRPEHIQTLKDIALLPFMMKTDLRDTYPFGLFACPMNDIVRFHC
                     SSGTTGKPIVIGNTQNDIRVWQNGCMRALALCGVRSSDIMQVAYGYGLFTGGLGLHYG
                     GEGLGCAVLPISGGNTDRQLMLMRDMGTTVIACTPSYFLHLIDEGMKKGIDFKRDMQI
                     RHGVFGAEPWTEEMRAKIESLTGIFAHDIYGLTEIAGPGVANDCRCRRGLHVFEDHFY
                     PEIVDPETLEPLPDGEQGELVFTTLTRTGTPMLRYRTRDISRLLTGACPCGRTIRAIE
                     RIHARSDDMLIIRGVNVFPSQIEAGLLSIDSVLPHYQIVVGKEGDLDTIEIKVEVTRE
                     TFSDDIKTLERLRQRIGQAVQRIINLSPHITLVEPHTLPRSEGKLQRVTDLRNRAVSS
                     KGV"
     gene            8004..8441
                     /locus_tag="PLZ74_06760"
     CDS             8004..8441
                     /locus_tag="PLZ74_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOR98058.1"
                     /translation="MKIQQISLFLENKPGHLNTICRTLADAGINIVTLSLADTQQFGI
                     VRLIVQEWQKAKEVLEQAGYVVNVREVVAAAVADRPGGMVEVLDIIGKTGVNIEYMYA
                     FAFRHNAEAVLVFRFDDPDRAVRALQAAGRDVLDAVTLFQEAK"
     gene            8438..9769
                     /locus_tag="PLZ74_06765"
     CDS             8438..9769
                     /locus_tag="PLZ74_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOR98059.1"
                     /translation="MNTTPIVENRFWQPEFECMPRPRILALQVERLRETVARAMRVPF
                     YRESFERKGITPDVIRSVEDVRRLPFTTKADFRDHYPLGLTAVPRSEIARFQGTSGTT
                     GKPTWTAYTAADVDRWADLCARFLVSGGLRPEHTVQISFGFGLFTGGFGLHNGIQRVG
                     AAVLPVSSGNAARQLMCMQDLQPEVLICTPSYALKLMDDILHGGVDRAALAIQHAHFG
                     GEPWTEEIRERIETGLGIRCWNNYGMAECGGPGLSGECAFRNGMHIQEDQFIVETIDP
                     ETLEPLPDGEKGELVVTPLCREALPVVRYRTRDIGILYADGPCPCGRTTRRMSRILGR
                     TDDMLIIRGVNVFPSQIETALMRFEKVAPHYMIEVTRPTALDEVTVHIEMQPGMFSDS
                     MREMQGIRERIAAEIYGVTGLHMRVNLVSPNTLERFEGKSRRVKDLRKLVD"
BASE COUNT         1852 a         2892 c         3218 g         1992 t
ORIGIN      
        1 tgtcaaggtg gtgctgacac cggagaagag gcggtacgga aagttgcagg tggtgatggt
       61 gacacttgcg gacaaggacg ggaacttcgt gccggacgac gaccgccgca tccattttca
      121 ggtggaagga tgccgaattc tcgccgtcgg caattccgat ccgaggggat atgaccgttt
      181 catgaagaca gattcgcatc cgctcaagtt cggacgggcg ggtatttttc tggagatcga
      241 gccgggcgcg aaatggaaac taacggcaac ggcggaaggg tgtgtcaaag cccaaaaagc
      301 ggcggagtga tttcctcgcg caaagtacgg ttggacgtct cggatgcccg aggacgttgc
      361 cgaatccgga agcttccagg ggtaggggaa cgggggtgcg cccaaccgta accgggggcg
      421 cactttttgt gaatggtcag cggctcaggc gataggcttc catcagcgtg tggtatttga
      481 ccagcatgtt gggaacctcc cagtcgggca tgtcacggtt ctgcagaaaa ccgaggaagg
      541 cttccatgac ttggccgaaa tgggcttcgt gaccgaccgc gtactgctcc gggacgatga
      601 tgcgccaacc ggtttcatgc ggttccgcgc tgatgccggg ataggtcttg gcgagcgttg
      661 cgactgcatc ggtcagggca cggccgagtt cctctttgtt gacacctgcg acgcggcggg
      721 gttcgacata gagcaccggt ttgtaccctt cggcctcgcc ctgctggatg atcagggaac
      781 tgcgtgtgcc gcgcatcagc gagtaatggg tgtcccccgc gccggccggg ggttggaaat
      841 tccacagcac cgaagctttg gcatggaccc cccggatcac ataggtgaat tcgccgttgg
      901 cacggcactg caacacgcct tgggcgtcga ggtctttttt caggtaatcc ggccaggcgg
      961 tctgcgaggt cgaggttttg aactgttcca gcgtgatcgg cgtgggccag acgcgcgcct
     1021 tcaacacttt gatgtccgca ggcgtgaggg ccacgccggg gaaggtctcc cattggatga
     1081 gatcggtcag gtgggtgttc acatccacga tcgcctcgcc ctgctgggtg gtgtcgtaat
     1141 accaaggcgg ccgctggagc ggcttgccgg ccacctgttt gcagaagtgg tggacgctct
     1201 ccttggtgac cgccggatcg tccggtgtgc ccttctcctg ctcgccgtac acggtgggga
     1261 accgtgacag ttcgcgttgc agcacgctgg tgatctcaaa gcgctcggtc atgatgtcgt
     1321 agagcaggac ccctttggag cgggcgactt cgaacgcttg tttgagtaac tcgtaatcct
     1381 tgggcgtgat ggccatcggt ttgtcggcaa gcacatggaa cccggcctgc acgcttttga
     1441 ggatgtattc ggttttgcgt gcgttgttgc cggccagcac cacgacgttt ccggcccgct
     1501 ccgcgatcat ccggtcaaag aagtcgggac cggtgtacac aacgctctcc caagaggtcg
     1561 ggtcttccgc gcgggtgttg aagccccgga tccggtcgag gtgcagggag aggtcggggc
     1621 cggccggtgc gtagacgtga accttcggcg agacgccggg gaacatgcgt ttctgcacca
     1681 gcgcggcatg gaaatggccg gggtccagag tgatcagttt cacggtgtcg cccccgcagg
     1741 tgttggggca gcagcgggtg ccggggttgt cgcaaccggt cagcgccaac agcgcggcgc
     1801 cggtgagcag cagggtgttt cgattcatgg tgtcctcgtt gggatggttt gaagaaaagg
     1861 cggagtgtaa cggactcata cgctgacgcg gatgtcggca gcctgaagca ctttcgggat
     1921 gctgaaggga ccgcgctcgg gacaggccag caatgcgttg gccccggcgt catcggtgcg
     1981 ttcggtacgc cagtcccagg cgagggtctt gccggttttc atgcagagat ggccgagggt
     2041 gcagaccgag gtcgagcggt ggccgatttc agcgttggtc accgggcttg tgcggttttg
     2101 gatcgcaagc agccagtctt tcacatggtt gtcgctgaca tgcagacgca cggcgttagc
     2161 gtgcagcggc gcttcaagga gccgcgggtc gctcgcatcc agcgccttga gggtcttgcc
     2221 gggcgtggtg gggtcgctgg cagtgacttt ggcggcgccg cggcttacga aaatccagcc
     2281 ttcatcgccg atgaagcgca cgccgttcgg atagcggtaa cagacactga ccttgatgtc
     2341 gtcgggatag gtgtagagca ggtcgaattc gcggtgcgca ttccagagcc ctttgtcgag
     2401 ccattggcag gtgcccgaaa cctttagggg cccagtctcc tcggtgccca gtccccactg
     2461 cacgatatcc atgtggtgtg cgccccagtt ggtgatcatg ccccagccgt agggttccat
     2521 ctgtatccac ccgggacggg aagcgatttg ggcgatgtcc tgattatgca cacggtcctc
     2581 ggtatagtac gcctccggtg tcggccccag ccacgtgtcg taatccagat tcggcggaat
     2641 gggcatctct tctttgcggc cgccgtcccg gtctttgccg aaaccgactt cgacacggcg
     2701 gatccggccg atccggccgt tacgcaccag ttcgcacgcc gtgcggaact gtttgctgga
     2761 acgctgctgg gacccgacct gcagcaccac ctttttgcgg cgcacgattt cggccagaat
     2821 acgcccctca tggatcgttt gtgaaaaggg cttctgcaac cagatgtctt ttcccgcaag
     2881 tgcgcattcg atggcgcaca aggcgtgcca gtgatccggc acgcagatca tcacggcgtc
     2941 cacgccgccg tcgcgcaaca gatcagaata gcggccgaaa gttttgacgg tatggttttg
     3001 caaaccgaga cgcttgttgt actcgttctc gacccgggcc ttcgaatggg ccagccgctt
     3061 ggagtcgaga tcgcagaggg ccgtgatccg cgccatctcg gtcaagccga aaagtccggg
     3121 catctccatg ctggtcgcga tccgtccgca tccgatgatg ccgatgtgga tctgcttgct
     3181 tggggcagcg gcaccgaaca gatgcggggg gatcaggttc ggcagggtaa tggctgcggt
     3241 cgtcgcgcct ccggcgcgaa gaaaggtgcg tctggtcagg gtggacatgg tgtgtttctc
     3301 ccctaggata atcattgagg cacatacggt aatcctgcgc gtgtccgcgc ggcgatccgg
     3361 tcgggcgcgg gcacacgtag gccgatatgc gcaaacaata ggccaaggag acgcttttgc
     3421 gcaagggttt tgttaccgag gcacacgatg gagacgaagg atgtttcccg cactttggtt
     3481 tctggtttct tgaaatcgcc atcgctatcg tttccccatc cccgtcctcg ttctcgtccg
     3541 cggacgcctc ggggaggtat ccctgccgaa acccggcacg gcctatctaa tctgtctgct
     3601 gaaccctgcc cgcctcaacg gccagtgact caaggatacg cccggacaaa ccgaagcgtc
     3661 acatcaaagg cttggttttt gccgttcgct ttgataaagt tcgcccattt ctgattcgtg
     3721 gggataattc ggcaacgcgg aattcatgat ggagacgatc ggacaaaagc ttttggatgc
     3781 gctcaagctg tatggcgtga aagtgctgtt tgcgctcggc atcctgctgg tcgggtggtt
     3841 cgtcgcccgg atgatccgtc aggcactcaa aaacctcatg cgtaaagcgc atctggacga
     3901 cacgctggtc acgttcctcg ccagtctcag ccataccgcg gtcatgattc tcgtcatcat
     3961 caccgcgctg ggtcaagtcg gcttgcagac gacctcgttc gtggccgtgc tcggtgcggc
     4021 ggggctggcg gtgggcttgg cgctgcaagg gtcgctctcc aacttcgctt acgggattct
     4081 gatggttttg ttcaagccct tccgggcggg cgactacatc gagggtgccg gcgtgggcgg
     4141 actggtcaag gcggtcggga tcttcaccac cgaattgcgc tccccggaca acaagacgat
     4201 catcgttccc aatgccaaga tgatgggtga caacattgtc aattactcgg ccaacgccac
     4261 acggcgtctg gaactgaaga tctgtgtgag tttcgcggac gacctcaaaa aagccaaaca
     4321 ggtgctggag tcggttctgg aggcagagcc gcgcgtgctc aaggatccgg ccccgtttgt
     4381 cggcgtgttt gcgttgagtg actacggtgt cgaattcacg gtccgccctt gggtgaaaac
     4441 cccggaatac tgggatgtgc ggtttgcgct gctcgaagcg atcaagacgc gtttggatgc
     4501 ggaggggatc accattccct tgccgcaaca ggttgtgcat ttgcatcgga atgaggaata
     4561 agaggggaga cggggccgcg gggcgcccct gcgccgtccc gggcatccgg aagaaaaagg
     4621 ccatgcgcgg gagaggggac agcggtcatg aaaagcaggc gtttctggca tacggcgggg
     4681 gttctgattg gtgtgtcggc tgcggtgtgg gtgtgcgctg cggctgaact ttcggggggg
     4741 cgcgaggatc cggacggcaa gaccgtctgg tacgacggcc agtccctgcc gatcgagggg
     4801 cgcgcctttc aggacaccga ggtgttttac gaccggttgc ccgcgcgcgc ccagaagacc
     4861 gtgacgccgg gtgtctgggg gaacagccgc cactcggccg gcatggcgtt ccgcctcgtt
     4921 accgatgcgc caaccgtcaa ggtgcgctgg ggggtgcgca atgcctcgct ggccatgccg
     4981 cacatgcccg ccacgggggt cagcggtgtg gatgtctacc gtcgtttgcc ggagggtggc
     5041 tggtatttcg tgcgcaacgg acgccccggg tcgttcacca actctctcac gtcgttcagc
     5101 atcaccccca acacggagtt tatgatctat ctgccgctct ataacggcac gtcgtttctc
     5161 gaggtgggga tccccaaggg taaccgcctt tttgcgccgc ccgtacgcgc cagcgggatc
     5221 accaagccgg tggtctttta cggcacctcg atcacgcacg gcggctgtgc ctcgcgaccc
     5281 ggtctggcgt ttacggccgt tgccggacgc ttcgcggacg tgccggtcgt caacctcggt
     5341 ttttccggca gcggtaagat ggagatggcg ctctgcgatc tgatcgcgga gatcgatgcc
     5401 tcggtgtatg tgctggattg cctttggaac atgggtgacg ctctggtgaa ggagcgggcg
     5461 gaacccttca tccgtgctct gcgcgaacgg cgtcccgata caccgattct gctggccgag
     5521 gattgcaacg tctttggccg cgccccaacc tccaaatccc tcctgatgcg ggcgatctac
     5581 gacaagttga aagcggagga tcccgcccga tgggctaagc tccactattt ggaggcaaag
     5641 gacatgctgg ggcatgatgg cgagggtacg gtggacggat gccaccccaa cgaccttggc
     5701 atgatgcgtc agggtttggt ctacggtcag gcgctgaaaa aagcgctcgg ccgctaaggg
     5761 aatgagcgtg atgcgggagc ggagtcatgt gggaatatag tgacaaggtg atggatcatt
     5821 tccgcaatcc gcgcaacgtc gggaagatcg aaaaccctga cggtgtcggc acggtcggtt
     5881 cgctagcctg tggtgacgcg ctgacgctga tgttccggct tgacgcggac gggcgtatcg
     5941 cggaagtgaa gttccagacg ttcggctgcg ccagcgctat cgcctcctct tcggcactga
     6001 cggagatgat actcggcaaa accttggagg aagcggagcg gatcacgaat cgggagatcg
     6061 cggaatacct cggcgggttg cccgaccaga agatgcactg ttcggtcatg ggccgcgagg
     6121 ccctggaagc ggccatccgc aattaccgca ccggagagac caccgttaaa aaacgcgatg
     6181 atcccatcgt gtgcacctgt ttcggtgtct cggagggcga gatccggcgt gtggtcacgg
     6241 aaaacgggtt gacgcaggtg gaggaggtga ccaacttctg caaggcgggc ggcggatgcg
     6301 gcatgtgccg cgatgacatc caaaaaacca tcgatgtgct gcgcgcgcag ccggtgggcc
     6361 cgtccgtagc caccgtggcc gcgcccgcaa agaaactcac caatctccag aagatgaagc
     6421 ggatcgaaga ggtggtcaac cgcgagatcc ggccccaact gcagaaggac ggcggcgaca
     6481 tcgagttgat cgatatcgtg ggcgactgtg tgaccatcgc gttccgcggt atgtgcgcgg
     6541 ggtgccactc tgccgcgttc acccgtcagg atttcatcga ggcgcggctc cgcgagttcg
     6601 ttgatcctgc cattaccgta atcgaagagg aaaaataatc atgtctcttt atgagtatgc
     6661 tcacgatctg gataccccgg tccatccggt cagtgctccg gattacacgc ctgtcgaaga
     6721 gttgcgcgcg ctgcagcttc accggctgca aggcctggtc cgccgggtct acgaccatgt
     6781 gcccctgacc cgcgcgcgca tggacgagcg aggggtccgg ccggaacaca tccagaccct
     6841 caaggatatc gcgttgctgc ccttcatgat gaagactgat ctgcgcgaca cctatccctt
     6901 cggcctcttc gcttgtccga tgaacgacat cgtgcgtttc cactgttcca gcggaacgac
     6961 cggtaaaccc atcgtaatcg gaaatacgca gaatgatatc cgggtctggc agaatggctg
     7021 catgcgggcg ctggccctgt gcggtgtgcg cagttcggac atcatgcagg tcgcctacgg
     7081 ctatggcctc ttcactggcg ggctcggtct gcactacggc ggcgaggggc ttggttgtgc
     7141 cgtgttgccg atctccggcg gcaacaccga ccgccagctc atgctcatgc gcgacatggg
     7201 gacgacggtc atcgcctgca ccccgagtta ttttctgcac ctgatcgatg agggcatgaa
     7261 aaaaggaatc gatttcaaac gggacatgca aatccggcac ggcgtgttcg gcgcggagcc
     7321 gtggaccgag gagatgcgag ctaaaatcga aagcctcacc gggatcttcg cgcacgacat
     7381 ctacggcctg accgaaatcg ccggaccggg tgtcgccaac gattgccgct gccgacgcgg
     7441 cctgcacgtc tttgaggatc acttctatcc cgagattgtg gatccggaaa ccctcgaacc
     7501 cctgcccgac ggcgaacagg gcgagttggt tttcaccacg ctcacacgca ccggaacgcc
     7561 gatgctgcgt taccgcacgc gcgacatctc gaggctcctc accggggcct gcccttgcgg
     7621 ccgcacgatc cgtgcgatcg agcggatcca cgcccgcagc gatgacatgc tgatcatccg
     7681 cggcgtgaac gtctttccct cgcagatcga ggccggactc ctgtccatcg actcggttct
     7741 gccccactac cagatcgtgg tgggcaaaga gggcgatctc gacacgatcg agatcaaggt
     7801 ggaggtcacg cgggagacct tcagcgatga catcaagacg ctggagcggt tgcgtcagcg
     7861 gatcggccaa gcggtccagc gcatcatcaa cctgagtccc catatcacac tggtggagcc
     7921 ccatacgctt ccgcgcagcg aaggcaagct ccagcgtgtc acggatctgc gaaaccgcgc
     7981 ggtatcgtcg aaaggagtat agtatgaaaa tccaacagat ttccctgttt ctggaaaata
     8041 agcccgggca cctgaatacg atctgccgca cgctggctga tgcgggcatc aatatcgtga
     8101 ccctctcgct ggccgacacc cagcagttcg gcatcgtccg gctgatcgtg caggagtggc
     8161 agaaggccaa ggaggtgctc gaacaggccg ggtatgtggt gaatgtgcgc gaggttgtgg
     8221 ccgctgcggt cgccgaccgg ccgggcggta tggtggaagt gctcgacatc atcggcaaga
     8281 ccggtgtgaa catcgagtac atgtacgcct tcgcctttcg gcacaatgct gaagcagtgc
     8341 tggtcttccg ttttgacgat cccgaccgcg ctgtccgggc actgcaggcc gcaggccggg
     8401 atgtgttgga tgccgtcacc ctatttcaag aggcgaaatg aatactacac cgattgtcga
     8461 gaaccggttc tggcagccgg agtttgaatg tatgccgcgg ccccgtatcc tcgccttgca
     8521 ggtcgagcgg ttgcgcgaga cagtggcgcg cgcgatgcgg gtgccgttct accgcgagag
     8581 cttcgagcgc aagggcatca cgccggatgt catccggagc gtggaggatg tgcgccgtct
     8641 gccgttcacc accaaagctg atttccgtga ccattacccg ctcggactca cggctgtgcc
     8701 gcgctccgag atcgcgcggt ttcagggaac cagcggcacc accgggaagc cgacctggac
     8761 cgcttacacg gcggcggacg tggatcgttg ggccgatctg tgcgcccggt tcctggtctc
     8821 cggaggcctg cgtccggagc acacggtgca gatctcgttc ggtttcggac tcttcaccgg
     8881 cggcttcggg ctgcacaacg ggatccagcg tgtcggcgcc gcagtcctgc cggtctccag
     8941 cggcaacgcc gcgcgccagc tgatgtgcat gcaggatttg cagccggagg tgctgatctg
     9001 cacgccctct tatgccctca agctcatgga tgatatcctg cacggtgggg tggaccgtgc
     9061 cgcactcgcc atccagcacg cgcatttcgg cggcgaaccc tggacggagg agatacgaga
     9121 acgcatcgaa accggactcg gtatccgctg ttggaataac tacggcatgg ccgagtgcgg
     9181 cggacccggc ctctcgggag agtgcgcctt ccgcaacgga atgcacatcc aagaggacca
     9241 gttcatcgtc gagaccatcg atcccgagac gctggaaccg ctgccggacg gtgagaaggg
     9301 cgaactggtc gtcaccccgt tgtgccgcga ggccctccct gtggtccgtt atcgtacacg
     9361 cgatatcggc atcttgtacg cggacggccc ctgtccctgt ggccgcacca cccgccgcat
     9421 gagccggatt ctcggtcgta ccgatgacat gctgatcatc cgcggggtga acgtcttccc
     9481 gtcgcagatc gagaccgccc tgatgcgttt tgagaaggtg gctccacact acatgatcga
     9541 ggtcacccgg cccacggcgc tggacgaggt cacggtccac atcgagatgc agccgggcat
     9601 gttttccgac agcatgcggg agatgcaggg gatccgggag cgtatcgctg cggagatcta
     9661 cggcgtcacc gggttacaca tgcgtgtcaa cctcgtgagc cccaatacgc tggagcgttt
     9721 cgagggcaaa tcgcgccgcg tcaaggatct gcgcaagttg gtcgactgat ccaacacgcg
     9781 taaacaggcg gcttgcggag cgccggcccg gacaagtgtc acttgtccgg gtcacgctat
     9841 gcgcggcaaa agccgtacac cgcgcagcgt ttcatgttca cgcacgaacg gggcgcaccg
     9901 atcccgatag cgataccgat tccgataccg attcgcacgc cccccaattg catt
//
LOCUS       DAOSRB010000100         9918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6600_length_9918_cov_5.652540, whole genome shotgun sequence.
ACCESSION   DAOSRB010000100 DAOSRB010000000
VERSION     DAOSRB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6600_length_9918_cov_5.652540"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..604
                     /locus_tag="PLZ74_06770"
     CDS             71..604
                     /locus_tag="PLZ74_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOR98060.1"
                     /translation="MVYASPRFFGCPKSPAADSSRWVLRIADRSLIKTGTGTLTLTGA
                     NTYTGGTVVNSGSLVTTNLSTLGDSPSVTLALGTTLEVSAGGETFNGDLTVNVSSNSA
                     SLLAVNGSLTLGPDSSLTVAPGSQFNRRKTYTIVTCAALNGTFGSVNGLPKAWFARYV
                     SNRVELFYAEGTVIRLY"
     gene            868..1776
                     /locus_tag="PLZ74_06775"
     CDS             868..1776
                     /locus_tag="PLZ74_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98061.1"
                     /translation="MRNRINMFFLFLVGFTFAIPTMATDMTWSNTAGDYDLGNAQNWG
                     GTLPEGANHTATISTKVTDPAWLKLSAVSTLQPYAITVKNDIDLDLGTGSVFTALYRV
                     YFNTDSALTLRSGTFGVDWESASGERFFMGDNGNGGGSITVDGPDAIITSSKKNTLQI
                     GTNRKDYRLNILNGATLQANITLGNTSLQASNNVVWIEGPGSKHLVPSDSTVAPIAIG
                     STAPHCSYILTNQASLVVERDAGIFVGSFYSAVPGGLAAMHVLDHSTVDTLGSLYIGN
                     YSGTNSLYVSGASTFNCAEFRLGENP"
     gene            1906..2931
                     /locus_tag="PLZ74_06780"
     CDS             1906..2931
                     /locus_tag="PLZ74_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98062.1"
                     /translation="MFRLEDGAWMQASASTVVGNDLATNNIAYIGTNAVFDALGAVTI
                     GNSASTAFNQMIVDAGTFYVTNNPSARNIAVGNRGFGNRLEVVNGATVYMTNVNFYVG
                     SNSDSNVLVIADGAKWREEEEGLAKRNWGIHGIGSYNGIIVDNATLELPQNILRIGNN
                     ANSSNNWVRIVNNSIVPIYRTIMGEQGTDNTLEVANSLFTMWGSLQVGHTTATSYRQR
                     LWVHGTNTYITVNEGLDIGPDCELKFTIEGEGFLSNTVMYVNSLWPTETVTADHPYTV
                     RIEAAHGAKPGVYTLMQSKSANNLHWGAEDLFKLEYDPKYIQIVQRDSSKLVVKVKHH
                     GMTVLLR"
     gene            3652..4095
                     /locus_tag="PLZ74_06785"
     CDS             3652..4095
                     /locus_tag="PLZ74_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOR98063.1"
                     /translation="MNGSINQLLMQKGCDIITIAPTATVYEAIALMNEKNIGSLLILN
                     ANGKFAGIFAERDVFRKVILAGKDPKEVQVKAVMTKKMVYVAPESTVDECMALMTEKR
                     IRHIPVLDDGQKVLGIVSIGDLVKFVASEQDALIKNLERYIEGSL"
     gene            4193..4750
                     /locus_tag="PLZ74_06790"
     CDS             4193..4750
                     /locus_tag="PLZ74_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOR98064.1"
                     /translation="MSDISSAPAAPCGCACTFDESDQALVAKLHEVIADYRDKPGGLI
                     PALQTAQSLFGYLPEPAIRLIADGFGKTVSEVAGVVTFYSFFTLHPRGKHLVRVCLGT
                     ACYVRGGKEVLDAFRKKLGIDVGETTPDRQFSLEVGRCFGACGMAPVVMVNDTVYQRV
                     KPARVGELIGQIISVSAAGGEKEHA"
     gene            4747..7905
                     /gene="nuoF"
                     /locus_tag="PLZ74_06795"
     CDS             4747..7905
                     /gene="nuoF"
                     /locus_tag="PLZ74_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOR98065.1"
                     /translation="MKSPTLKKLRDVSELDAWRAKLQAAAGATGADAAPRQILICFGG
                     SCLASGAKEVRDALAAVLKREGLAEQVALVGTGCMGPCVIGPVLMITEDRTFYQGVKP
                     EDAEEIVTEHLKAGRRVERLLVSDPSGQTPAPCRESIEFFKRQTQIVLRNCGWVDPGR
                     IEDTIARDGYAALAKILSGMKPDEVIAEMKASGLRGRGGAGFPTWLKWHLATQTPEGE
                     RYVLCNADEGDPGAYMDRSVLEGDPHSVIEGMAIAAYAIGARQGYVYVRAEYPLAVER
                     LTAAIEQAHACGLLGEKILGSTFSFDLEIRMGSGAFVCGEETALIASIEGRRGEPRPR
                     PPFPAQKGLWGRPTVLNNVETYANVAAILLRGGAWYAGIGTPKSKGTKVFALAGAVRN
                     TGLVEVPIGTPLGEVVYDIGGGIRNNRHFKAAQIGGPSGGCIPRENLNVPLDFETLAE
                     LGAIMGSGGLIVMDEDTCMVDVARFFIEFVQEESCGKCAPCRVGTRRMLEILDRICSG
                     QGEEGDIERLIELGEFIRSASLCGLGQTAPNPVLSTIRHFRHEYEEHIRDRHCRAGVC
                     AGLVRAPCLSACPAGVDVPGFVSLVGDGRYAEALRLHREKNPFASVCARVCFHTCEEK
                     CRRASLDEPVAIRGVKRFMVDQEVIIQVPEVRENAVNAQRKIAIVGAGPAGLSCAYFL
                     ARMGYKPTVFESEPRPGGMLVQAIPAYRLPREELAREVRMIERLGVSIETERKLGRDF
                     TLQDLREQGFEAVFLAMGAALGVTLDLPGADAQGVTEAMRFLRQYNIRGSVPVGKRVA
                     VIGGGNAAIDAARTAIRLGAESVTVLYRRTREEMPAYAEEIEEALQEGVVLRTLIHPE
                     AFEVKNGKVTGVRCHPMRLGEFDRSGRRRPEDAVDAEGEVIPADQVIAAIGQALDAEA
                     LAGAVSVERNLRGWIAADPRTGATSVPWLFAGGDAVTGPSSVVEAIAAGERTAVAIDH
                     MLTGEEHAFWRGYCDAGANFDPNADPVPYAREKLPTIALEKRRQNFTEVEQPWNEATA
                     LRQAKRCLRCDYGKQPCGIENA"
     gene            7919..9688
                     /locus_tag="PLZ74_06800"
     CDS             7919..9688
                     /locus_tag="PLZ74_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOR98066.1"
                     /translation="MLNVTINLQTIQVPEGTTLLQAAGMIGVRIPTLCNLEHREPIGA
                     CRVCLVEVEGARTLMAACSTPVSEGMKVRTHSARARAARRQVVELLLSEHDGDCPTCD
                     RADDCELRALATELGVAVVPFQGVRAKPHRDVSTPALVRDNGKCIKCRRCVSICSEVQ
                     GVGALFPQGRGFQTVIGPAFTRDLDSVACVQCGQCAAVCPVGAIVEKSSIPEVWKALE
                     DPTKHVIVQTAPAIRAALGECFGYEPGTRVTGKMAAALRRLGFDGVFDTNFTADLTIM
                     EEGTELLTRLKKALVDKEPVALPMFTSCSPGWINFAEFYFPQFLPNISTCKSPQQMFG
                     AVAKSFYAEKIGKKPEEIVVVSVMPCTAKKFEAQRPEMSSSGVLDVDYVLTTRELARM
                     IKEAGIEFTSLPDEAMDAPLGLSSGAADIFANTGGVMEAALRTAYEIVTGRELPVENL
                     HIQAIAGLDGVKEAALTITGTVKEWSFLEGVELKVAVSHGLSNTRRLLEHIKAHGSPY
                     HFIEVMTCPGGCIGGGGQPRFTDDGVRLKRMAAIYSEDESKSLRKSHENPDVQKLYKE
                     YLGKPLGEKSHHLLHTHYASKAR"
BASE COUNT         1914 a         2837 c         3214 g         1953 t
ORIGIN      
        1 cgagtatctg aaggaaaagg gtttcggaca ggactgagtg tcttcccgaa cgtattgaaa
       61 accggggcgg atggtttacg catccccccg gttttttggt tgtcccaagt ctcctgccgc
      121 ggactcatcc cgttgggttc tgcggatcgc cgatcgctct ctgatcaaaa ccgggacggg
      181 caccctgacg ttgaccggtg ccaacaccta tacgggcggc acggttgtga acagcggttc
      241 cctggtgacg acgaacctct ccacactcgg cgactctccc tcggtcacct tggcgttggg
      301 gacaacgctg gaggtctcgg caggcggcga aacgttcaac ggggatctga cggtgaatgt
      361 ctcctccaac tcggcgagcc tgctggccgt gaacggcagc ctgacgctgg ggccggacag
      421 ttctttgacg gtggcgccgg gttcgcagtt caaccgcagg aagacgtaca cgatcgtgac
      481 gtgcgccgcg ctgaacggca ccttcggttc ggttaacggt ctgccgaaag catggttcgc
      541 gcgttatgtg tccaatcgtg ttgaactgtt ctacgcggag ggcacggtga tccggctgta
      601 ttagaacggt ttcggcgctc cggctccgcg ccgctggcgc ggagctgtgg acgtcccgcc
      661 gtactgcatg gaccgcctgc ggttcggacg cccgaaggta tggtaaaacg gctaggatca
      721 tcgtgccgaa gatcgtcacc ccaacttcgc cactttcaga ggttgacacg tttcggcttt
      781 tctccgcata atagctggcg ttcgggcgtt tgagggaacc gttgcggttc cggtctgtcc
      841 gaagcgatga aatcaaaaga ggtgatcatg cgtaaccgta tcaacatgtt ttttttgttt
      901 ctcgttgggt tcacttttgc gataccgacg atggcaacgg acatgacctg gtccaatacc
      961 gctggtgact acgacctggg taatgcccaa aactggggtg gaacgctgcc ggaaggtgcc
     1021 aaccacacgg cgacaatcag cacgaaagtg actgacccgg cttggctgaa actgtcggca
     1081 gtctcgacgc tccagccgta tgcgatcacg gtgaagaacg acatcgacct cgatctcggc
     1141 acggggagtg tgttcaccgc gctttaccgc gtatatttca acacggactc cgcactcacg
     1201 cttaggagcg gcacgttcgg cgtcgattgg gagtccgcga gtggcgaacg tttctttatg
     1261 ggtgacaatg gcaacggcgg cggctccatc accgtggacg ggccggatgc gatcatcaca
     1321 agctccaaaa agaacaccct ccagatcggc accaaccgta aggactaccg gctcaacatt
     1381 ttgaacggcg ccacattgca ggcgaacatc actttgggca acacgtcgct tcaggcatcc
     1441 aacaacgtcg tctggatcga aggccctggt tcgaaacacc tcgtcccatc cgattcgacg
     1501 gtcgctccaa tcgccatcgg atcgaccgcc ccgcactgca gctacattct cacaaaccaa
     1561 gcctcgctcg tcgtcgagag ggacgccggt atctttgtcg gcagtttcta ttcggctgtg
     1621 cccggcggac ttgcggcaat gcacgttctt gatcattcga cggtcgatac cctcggcagc
     1681 ctctacatcg gcaattacag tggcaccaac tcgctttacg tctccggggc ctcgaccttc
     1741 aactgcgccg aattccgtct cggtgaaaat ccctagagca agaccgctct taaaaatgcc
     1801 ggcgttggcc cctccttcgc taataccgca acgatacgcg gcgacggaac gcgccttgca
     1861 gcctccggta atgtctatat cggcggcacg tacacgcagt cgaacgtgtt ccgcctcgaa
     1921 gatggcgcgt ggatgcaagc ctccgccagc acggtggtcg gcaacgacct ggcgaccaac
     1981 aacatcgcct atatcggcac gaacgcggtg ttcgacgcgc tcggtgcggt cacgatcggc
     2041 aacagcgcct cgacggcgtt caaccagatg atcgtggacg cgggtacgtt ttatgtcacc
     2101 aacaacccgt ctgcccgcaa tatcgccgtc gggaaccgcg gcttcggcaa ccgtctggaa
     2161 gtcgtgaatg gcgcgacggt gtacatgacg aacgtcaact tttacgtcgg atcgaattcc
     2221 gattccaatg tcctcgtcat tgcggacggc gccaagtggc gtgaagagga ggaagggctg
     2281 gcgaagcgca actggggcat ccacggcatc ggatcgtata acggaatcat cgtcgacaat
     2341 gcgacgctgg aactccccca gaacattctc cgtatcggga acaacgcaaa ttcgtccaac
     2401 aactgggtaa ggatcgtcaa caactcgatc gtgccgatct atcgcaccat catgggcgag
     2461 caaggtacgg acaacacact ggaagtcgcg aattcgctct ttaccatgtg ggggtcattg
     2521 caggtcgggc acacgaccgc gacctcttac cgtcagcgtc tctgggtgca cggcacgaac
     2581 acctacatta ctgtaaacga aggcctggat atcggtccgg attgtgaact caagttcacc
     2641 atcgaaggcg agggcttcct ctccaacacc gtgatgtatg tgaattccct ctggccaacg
     2701 gaaacggtca ccgctgatca tccgtacacg gttcggatcg aggcggctca tggcgccaaa
     2761 cccggcgtct acacgctcat gcagtcgaag agcgccaaca atctccactg gggagccgaa
     2821 gaccttttca agcttgagta cgatccgaag tatatccaga ttgtccagcg ggacagttcc
     2881 aaactcgtcg tcaaagttaa gcaccatggc atgacggtcc ttttacgctg atctggcgag
     2941 aagtctcctt ttgatttcgg gtgtttggaa ccgtcaagcg cgctcaagat tttgggtttc
     3001 tggttttatc ggttccatcc aaatcggggt cggggtcgct atcgggatcg ggatcgactg
     3061 tttcaccacg gaacaccacc gcctcaccgc ctcaccgcct cacagcctca ccgcctcaca
     3121 gtctcacagc ctttgagatt gcgggctccg cccgcatctc aatacggtgg ggtggtgacg
     3181 ggggcgttaa actttaagcc aggattacag gattaacagg attctgattg aggttatcgg
     3241 taagccggcc gtccggggtt ccgggcgaga acggggtggt gacggggacg cttacccgta
     3301 tgcagtgaag agggggggag gtgcgcgggc ggatgcccgc tggcgggtgt tggggcaggc
     3361 agacgctcgt ccctcccgat ctgagacatc gctatcggat cgatacgttc aaggtgactt
     3421 gcaagggtaa atgcacggag gcggaagagc ggagcgatga tttttatgcg gcgaggttcc
     3481 aagaagccct tgccgggtgt cgcaatatcg tttacaattt ccagtacagt gatggtgggg
     3541 aatgtgaatg gtctgcaccc atctcatcca cctcgctgta ttggaccggc atcaacggga
     3601 agagtccggg ctgacgggac aatgaaacgg taaaacgggg aaggatgcga aatgaacgga
     3661 tcgatcaacc aactgcttat gcaaaagggg tgcgatatca tcaccatcgc gccgacagcg
     3721 accgtatatg aggcgatcgc gctgatgaat gagaaaaaca tcggctctct cttgattctg
     3781 aatgccaacg gcaagttcgc gggaattttc gctgaacgcg atgtgttccg aaaggtgatt
     3841 ctggccggta aagacccgaa agaggtgcag gtcaaggcgg tcatgaccaa gaagatggtc
     3901 tatgtggcgc cggagagcac ggtggacgag tgtatggcgc tgatgactga aaagcgtatc
     3961 cgccatatcc cggtgttgga cgacggccag aaggtgctgg gcatcgtctc gatcggggat
     4021 ctggtgaaat tcgtggcctc cgaacaggat gcgctgatca aaaatctcga acggtatatc
     4081 gaaggttccc tgtaaggacg ttttccgtcc gttttggagc agatcgggac tgtttcaggc
     4141 gcgggcggtt tttcggttca acgttccccc gcaggggaca acggaggaaa gaatgagtga
     4201 tatctcttcc gctccagccg caccgtgcgg ctgcgcgtgc acatttgatg aatccgatca
     4261 ggctctggtc gcgaaattac atgaggtgat cgccgactac cgcgacaagc ccggcgggct
     4321 gattcccgcg cttcagacag cccaatccct tttcggttat ctgcctgagc ctgctatccg
     4381 cctgatcgct gacggtttcg gcaaaaccgt cagcgaagtg gccggtgtgg tcacgttcta
     4441 ttcgttcttt accctgcatc cccgcggcaa gcatctggtg cgcgtctgtc tcggtacggc
     4501 ctgctatgtg cgcggtggta aagaggtgtt ggacgccttc cgtaagaagc tcggaatcga
     4561 cgtgggggag accactccgg accgtcagtt ctctctggag gtggggcgct gtttcggcgc
     4621 gtgcggcatg gccccggtgg tcatggtgaa cgacacggtg tatcagcgtg tcaaaccggc
     4681 ccgtgtgggt gaactgatcg gtcagattat ttcggtatcc gccgcaggcg gggaaaagga
     4741 gcacgcatga agagcccgac gctgaaaaaa ctgcgggacg tttccgaact cgatgcttgg
     4801 cgggcgaagc ttcaggccgc ggcgggtgcc acgggcgccg acgccgcgcc gcgccagatc
     4861 ctgatctgct ttggcggcag ttgcctcgcc tccggtgcca aagaggtgcg cgacgctctg
     4921 gccgccgtgt tgaaacgcga ggggctcgcg gagcaggtgg cgctggtcgg caccggctgc
     4981 atgggcccgt gcgtcatcgg tcccgtgctg atgatcactg aggaccgtac tttttatcag
     5041 ggcgtcaaac ccgaagacgc cgaggagatc gtcaccgagc atctcaaggc tggccgtcgg
     5101 gtcgagcgac tcttggtgag cgacccttcc gggcagactc ccgcgccttg ccgcgaaagc
     5161 atcgagtttt tcaagcgcca gacgcagatt gtgttgcgta actgcgggtg ggtcgatccc
     5221 ggccgtatcg aagacacgat cgcgcgtgac gggtatgcgg cgctggccaa gatcctctcc
     5281 ggtatgaagc cggatgaggt gatcgcggaa atgaaggcct ccggattgcg cggccgtggc
     5341 ggtgccggat tcccgacgtg gctgaagtgg cacctcgcca cccagacgcc cgagggcgaa
     5401 cgctacgtgc tctgcaacgc ggatgagggc gacccgggcg cgtacatgga ccgcagcgtg
     5461 ttggagggcg atccccacag cgtgatcgag gggatggcca tcgccgccta cgccatcggc
     5521 gcgcggcagg ggtatgtcta tgtgcgggcc gaataccctc ttgccgtgga gcggctcact
     5581 gccgccatcg aacaggcgca cgcctgcgga ctgttgggcg aaaagatact cggttcaacg
     5641 ttttcgttcg atctcgaaat ccgcatgggc tccggggcgt tcgtctgcgg cgaggagacc
     5701 gcgctgatcg cctccatcga gggcaggcgc ggcgagccgc ggccgcggcc gcctttcccg
     5761 gcccagaagg ggctgtgggg gcggcccacg gtgctcaaca atgtcgagac ctacgccaac
     5821 gtggccgcga tcctgctgcg gggcggcgcc tggtacgccg ggatcggcac gcccaagagc
     5881 aagggcacca aggtgttcgc gctggctggc gcggtgcgca acaccggact tgtggaggtg
     5941 cccatcggaa ctccgttggg cgaggtggtc tatgatatcg gtggcggcat ccgcaacaac
     6001 cgccatttca aggcagccca gatcggcggg ccgtcgggtg gttgcatccc gcgcgaaaac
     6061 ctcaatgtgc cgcttgattt cgaaaccctc gccgaactcg gcgcgatcat gggttcgggc
     6121 ggtctgattg tgatggatga ggacacctgc atggtggatg tcgcgcggtt cttcatcgaa
     6181 ttcgtgcagg aggagtcgtg cggcaagtgc gcgccctgcc gggtcggtac acgtcgtatg
     6241 ctcgaaatcc ttgaccgcat ctgttccggt cagggcgagg aaggggatat cgagcggctc
     6301 atcgagttgg gcgagttcat caggtccgcc tcgttgtgcg gactcggcca gaccgcgccg
     6361 aatccggtgc tctccacgat ccgtcacttc cgccacgaat atgaggagca catccgcgac
     6421 cggcattgcc gcgcaggggt ctgtgccggt ctggtgcgcg cgccctgcct gagcgcctgt
     6481 cccgcagggg tcgatgtgcc aggcttcgtt tcgctggtcg gcgacggacg gtatgccgag
     6541 gcgttgcggc tgcaccgcga gaaaaacccc ttcgcatcgg tttgcgcgcg cgtctgcttc
     6601 catacctgcg aagagaaatg tcggcgcgcc tcgctggacg aaccggtcgc tatccgaggc
     6661 gtcaagcgtt tcatggtgga ccaggaggtc atcatccaag tgccggaagt gcgcgagaac
     6721 gccgtaaatg cgcagcgcaa gatcgcgata gtcggcgcgg ggccggcggg tctctcatgc
     6781 gcctatttct tggcgcggat ggggtacaaa ccaacggttt tcgagtctga accgcggccg
     6841 ggtggtatgc tggtgcaggc cattcccgcc taccgcctgc cgcgtgaaga gttggcgcgc
     6901 gaagtgcgca tgattgaacg ccttggtgtg agcatcgaaa ccgaacgcaa actgggccgc
     6961 gatttcaccc ttcaggacct gcgcgaacag gggtttgaag cggtctttct cgcgatgggc
     7021 gcggcattgg gcgtaacgct tgacctgccc ggcgcggatg cgcagggtgt gaccgaagcc
     7081 atgcgttttc tgcggcagta caatatccgc ggttcggtgc cggtgggcaa acgggtggcg
     7141 gtgatcggcg gcggcaacgc ggccattgat gcggcccgca cggcgatccg cctcggggcc
     7201 gagagcgtta ccgtgctcta ccggcgcacg cgcgaagaga tgcccgccta cgccgaggag
     7261 atcgaggagg cgctgcagga gggggtcgtt ctccgcacgc tgatccatcc cgaggccttc
     7321 gaggtcaaga acggcaaggt gacgggagtg cgttgccacc ccatgcggct cggcgagttc
     7381 gaccgctcgg gccgtcggcg tcccgaggat gcggtggatg ccgagggcga ggtgatcccc
     7441 gcagatcagg tgatcgcggc catcggtcag gcgctggatg cggaggcgct ggctggcgcg
     7501 gtttcggtcg aacggaatct gcgcggctgg atcgccgcgg atccgcgtac aggcgccact
     7561 tcggtgccct ggctctttgc gggcggggac gccgtgaccg gaccgtcgtc tgtggtggag
     7621 gcgatcgccg cgggcgaacg gaccgcggtg gccatcgacc acatgctgac cggcgaggag
     7681 cacgccttct ggcgcgggta ttgcgatgca ggtgcgaatt ttgacccgaa tgcggacccc
     7741 gtaccctatg cgcgggagaa gttgccgacg atcgcactgg aaaaacggcg gcagaatttc
     7801 acggaggtcg aacagccgtg gaacgaggcc acggcattgc gtcaggccaa gcgctgtctg
     7861 cggtgtgatt acggcaagca gccctgcgga attgaaaacg cctaatttgg agaagcccat
     7921 gttaaatgtg acaatcaatt tgcagacgat ccaggtgccg gaaggcacca ctcttcttca
     7981 ggcggcgggc atgatcgggg tgcgcatccc gacgctctgc aatctggagc atcgcgaacc
     8041 catcggcgca tgccgtgtct gccttgtgga agtcgagggg gcgcgcacac tgatggcggc
     8101 gtgttccacg ccggtttccg aaggcatgaa ggtgcggacg cacagtgcgc gggcgcgcgc
     8161 tgcgcggcgt caggtggtgg aactcctgct gtcagagcat gacggcgact gtcccacgtg
     8221 cgaccgcgcc gacgattgcg aattacgcgc gctggcgacg gagttgggcg tggcggtcgt
     8281 gccgtttcag ggggtgcgcg ccaaaccgca tcgggacgtt tccacgcctg ctttggtgcg
     8341 cgataacggc aaatgcatca agtgtcggcg gtgcgtttca atctgcagcg aggtgcaggg
     8401 ggtcggcgcg ctcttcccgc agggccgcgg cttccagact gtcatcggtc cggccttcac
     8461 gcgggatctc gacagcgtgg cttgcgtgca gtgcggccag tgtgccgcgg tctgcccggt
     8521 gggcgcgatt gtcgaaaaga gtagcatccc cgaggtttgg aaggcgctgg aggatccgac
     8581 gaaacacgtg atcgtgcaga ccgcgccagc catccgtgcc gcgttgggcg aatgtttcgg
     8641 ctacgagccc ggtacgcgcg tgaccggcaa gatggccgcc gcgctcagaa ggctggggtt
     8701 cgacggcgtg ttcgacacca atttcacggc ggatctgacc atcatggaag agggcaccga
     8761 actgctcacc cgcctgaaga aggcgctggt cgacaaggag cctgtggcct tgccgatgtt
     8821 cacgagttgc tcgccgggct ggatcaactt cgcggagttc tatttcccgc agtttctgcc
     8881 gaacatctcc acctgcaaat cgccgcagca gatgtttggc gcggtggcca agagttttta
     8941 tgccgagaag atcggcaaaa agcctgaaga gattgtggtg gtctcggtca tgccttgcac
     9001 cgccaaaaag tttgaggccc agcgtccgga aatgtcttcc agcggtgtgc ttgacgtgga
     9061 ttacgttctc accacgcgtg agcttgcgcg gatgattaag gaggcgggga tcgagttcac
     9121 gtcgctgccg gatgaggcga tggacgcgcc gctggggctc tcctcgggcg cggcggacat
     9181 tttcgcgaac accggcgggg tcatggaggc cgcgctgaga accgcgtacg agatcgtgac
     9241 cggccgcgag ctgccggtcg aaaacctgca tatccaagcc atcgcgggtc tcgatggggt
     9301 gaaggaggcg gcactgacca ttaccggcac ggtgaaggag tggtcgtttt tggagggggt
     9361 cgagctgaag gtggcggttt cgcacggcct cagcaacacg cggcgtctgc tggagcacat
     9421 caaggcccac ggctcgccct accacttcat cgaggtcatg acctgccccg gcggttgcat
     9481 cggcggcggc ggacaaccgc gctttacgga tgacggcgtg cggctgaaac gtatggccgc
     9541 gatttacagc gaggacgaga gcaagagcct gcgcaagtcc catgagaatc cggacgtgca
     9601 gaagctctac aaggagtatc tgggcaagcc gctcggtgaa aaatcgcacc atctgctgca
     9661 cacacactac gcatccaagg cgcggtgagg tcagattgag gaaacgctca acactggcat
     9721 aaccaccccc ggtcctgttg ataaggttgg cctcacgggg ccttgagggg cccccggcca
     9781 acttaccaac aggcgtgtgg gggtggttat acaagatgcc cctgcttttc aggggtttct
     9841 ggtttcaggg tttcatggtt tcaggtttca gggtttcatc caaatcgggg tcgggatcgc
     9901 tatcgggatc gggatcga
//
LOCUS       DAOSRB010000101         9900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6609_length_9900_cov_5.275775, whole genome shotgun sequence.
ACCESSION   DAOSRB010000101 DAOSRB010000000
VERSION     DAOSRB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6609_length_9900_cov_5.275775"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3466
                     /gene="drmA"
                     /locus_tag="PLZ74_06805"
     CDS             <1..3466
                     /gene="drmA"
                     /locus_tag="PLZ74_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DISARM system helicase DrmA"
                     /protein_id="HOR98067.1"
                     /translation="NVRTKLVEAIRLDLVGPDNDHPFAAELLPESPSRWYLTGFLVPT
                     EAPLEQKFDETSAEEIDSPAEAGGMDDSVNTDRPAANRRSLLPSSIGLSVLVPKDTRK
                     LDATVVWGDYVEEEPDGETAPGEGGVPPASDGDMPGEVKERSHDGGKPRHWRRTPRTE
                     HVAIELPEPGQRPRRLTVPDSGGLKLVVTVREAGTSVLPAGTRAVSVFLVNYRTPDAL
                     QPYRAFIFQAGIRLECREAFVPRPDPRGWEPDEEWDERVADLQYRDSVEHVVGHGVSG
                     HAEVGDDGACRVVESVWIPQGEVERIAPSSIPDVELGMEVLAELADGADAKAKLGKLV
                     TRYKEWIGRQRSGLSGLNANRTQTANDMLVEAANSADRIAAGIDLLDDPDVLTAFRLA
                     NKAVARSLRQRFGQIQGKAPKAVDAPRWRPFQLAYLLMTMRGVVNPKHGDRDVVDLIF
                     FPTGGGKTEAYLALAAFTLVLRRLRSRDANGKPTIQGAGVSVLMRYTLRLLTLDQLGR
                     AASLICALELERQAHPELLGEWPFEIGLWVGQAATPNRMGHRNDRSAQAPYTAYTKTK
                     QFSRDDSKPSPIPLEECPWCGTKFTRNSFRLVPNENKPLDLRIHCTNEHCEFCGDRYL
                     PVVAVDEPIYRRLPCFMIATVDKFAALPWTGQTGTLFGKVSRFDKDGFYGPCDPGKGV
                     PLPGGCLPPPDLIIQDELHLISGPLGTVAGLYETAIDALATTALDEMSIRPKVVASTA
                     TVRRADSQIRALFNRSRVSVFPPPGPDRRDSFFAHTQHAHEEPPRLYIGIAAQGRSLK
                     VVLLRAGLALLSAGQTAWDEAGDNSDQNPADPYMTLLGYFNSLRELGGSRRIIEDELR
                     SRLAEYGQRRRIQPVDRLFSNRIIAYEVLELTSRVPTNDVAAAKRRLALPFASDERVD
                     VALATNMISVGLDITRLGLMVVLGQPKTSSEYIQATSRVGRDSKRPGLVVTLLNIHKP
                     RDRSHYERFAVYHSHFYRNVEATSVTPFSPRALDRALPAVLVALCRQIIPDLTPARAA
                     KDILTLRDRLGPLCQLIAARAENHNGDLSPADRTALAAKVLNRCNSLLDDWLWIAKQA
                     QDEGSGLQYQREETSPPRRLLYDFLDPEVPNLAPAQRRFRANRSMRDVEASVDIRVKN
                     LNDWGAKP"
     gene            3463..5271
                     /locus_tag="PLZ74_06810"
     CDS             3463..5271
                     /locus_tag="PLZ74_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOR98068.1"
                     /translation="MKNPQIRSSQLITTFGPGAMVDLPKDAVIIAGLDNWHYSRRLPL
                     PLVSEPRLQRKLADIIGLPTVALRKPPPPIEERGFTPDIIGYRFPEWFIVQKAVIRNR
                     GGRICRARRLVNLKDLDGGRYRDRDGKKYPVVPVRFVRACRRGHVGDIDWWTFVHGDD
                     RDCRRGLYMEERGTSGDLDSVWITCECGAERPMSHAARMELKALGHCNGSRPWLGPGS
                     REACGDQNRLLIRSASNAYFPQLLSVISIPDSLTAVDQAVAALWESHFSAVATEAQLA
                     FVMTMPAVKQQLDGFTPEQVWTVIERRRRSGDEDERPIKEVEFEVLAQAKDEIGEDKP
                     EGDFYARTLPSAVWADAWTDVIERVVLVHRLREVVAQLGFTRFDAAGPDITGELDIDV
                     QRAPLAIDCSWLPTIENRGEGVFIQFKPEAIAAWLDQPAVQQRDKALQSGFDQWLGEH
                     ENSKHKYPGLPYYMLHSFSHLLLIAMSLECGYPASSIRERVYAMPSQYGVLVYTGSPD
                     AEGTLGGLVEAGRRIKFHIGRALDMGMLCSNDPVCAFHSPRSQDHQPLLGSACHGCLL
                     ISETSCEQRNNFLDRALVVPTVEQLGTEFFRTIPHA"
     gene            5264..6070
                     /gene="drmC"
                     /locus_tag="PLZ74_06815"
     CDS             5264..6070
                     /gene="drmC"
                     /locus_tag="PLZ74_06815"
                     /inference="COORDINATES: protein motif:HMM:NF038319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system phospholipase D-like protein DrmC"
                     /protein_id="HOR98069.1"
                     /translation="MRDVFHKLTSGSLCQLAASLRGGELSRGITTHAVRQIAGGVLVG
                     DLAIALTALREDGWLPSQIAELAETVVESRARVAQPEELFDLVLSGPDVSGVPTRETA
                     AVIHALIEEAQREVIVVGYVIHNGKKLFERLAEKAGETPGLDLWLCFNIQRPYKDTSL
                     SSEIVRRFANKFAKDEWPWRPLPPVYYDPRLLEDGTTQSSLHAKCLIIDRKAALITSA
                     NFTEAAHKRNIEAGVIVRYEPLVIRIATYFDTLRQTLFAPVDFSCHQGCP"
     gene            6228..9713
                     /locus_tag="PLZ74_06820"
     CDS             6228..9713
                     /locus_tag="PLZ74_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98070.1"
                     /translation="MTARDVFWMTGNAICCVVLHTVAADGSWTNRVGGKWNEPGNWLG
                     GVVAEGEGNTAFFTNAPSAKCLVRVPSAGVTLQNLLFSGFSNAFYLFDGFVTLEGGNP
                     QVGVTNPNDSNNANNYNTDLRGTNGVSFYGGTLGMDTSYTGPTRLCGKNKTLNTFMHF
                     YVAERGVAPSNFLSGGTLIFDGGHLRLEGCKAKAGGTGVFTLTEGDTLIHWKSGTTPW
                     AGFPVSGNGVPDGAFCRQQIHDQAYILSHPATASGDFSLSIVRNSGSLAYQWFDQIDM
                     QAYGILTIVVSGYETQIETDTIAGRERFFRIIGSGNNSTNLAIFRMRGANGFPGAIEA
                     SAVKVEALAQTNGVNPEIPDLTVAATTILTVPEPEAVLSVPGVTLIEGELAKEGAGTL
                     ALQHVYGADPRVCVRGGTLALAAPVVPEPAPVGGAWFHADASATASLVTEQENGTNFV
                     VCWRDVRGNGIYATNDVANSGPRPFLRSACQNGLPVVDFGSYHYPAKGVVGYGAYLNW
                     SEMWTKIYECYFVYSDTEDTASLSAGTYWPTPVGNSNHNAFFARGVNGAILGGPLAQE
                     EVLSGVISLDHATVPAATVVPSGFHVVSFVATNDVRAGAFARERVNSYGGVRIAEVLV
                     FTNRLTVAARREITDYLSRKWFGREPGIGALTLDNGSAVRVADGETVRVGALCVGKPA
                     VKTGGGVLSAETVSGDSLVISNGTVRQTGAAGLLSGGVSCVGSATLDVADEAGSVTAG
                     WIDAEGELSKAGEGELTVATLGVGVTSLHVRGGALNVTGVDPAYGQDAVFHVDADDAA
                     SLETVAVNGTNFVTRWNDCRTNGFYAAAQDTVHRPFLSPGQQNGRSVVDFGAFTKDTN
                     AVYTAWLNWKEYKHSVRDVFVVVRDTDPSKQATLVGNQNHYLDFMRGASGALVGEHPH
                     SEPIRNGSCAIDGTSVTYTARLPDGFHVVSFFPTNTVRASAFGRERFNGFGGHQLAEA
                     LFFTRELSANERQNIHAQLLSKWLGQTLHRAFDHILVENGGVCSITNMTLATDLLEGT
                     GTIRAQGLTADALSAGTTETAIGSLAVSGGLTLPDGAVVDVDCNAFACDRVDVQGTLT
                     IGGGGTVRLRGDGGDIGGKVFTLFSFDTCAGGENLTLWQVTGEITARYVVRLWIEDNQ
                     IRLGVKGKGTQVLLR"
BASE COUNT         1850 a         2832 c         3316 g         1902 t
ORIGIN      
        1 gaacgtccgg acgaaacttg tcgaagcgat ccggcttgac ctcgtcgggc cggacaacga
       61 tcatccgttc gctgcggaac tgctgcccga atcgccttcg cgctggtatc tgaccgggtt
      121 ccttgtgccc accgaagcgc cgctcgagca gaagttcgac gagacgtccg ccgaggagat
      181 cgactccccg gcggaggcgg gcggtatgga tgactcggtg aacaccgacc gtccggctgc
      241 gaaccgccga agtctgctcc cgtcgtccat cgggctgagc gtgctggtgc cgaaagacac
      301 acgcaagctg gatgcgaccg tcgtctgggg ggactacgtc gaagaggagc ctgatggcga
      361 aacggcaccg ggggagggcg gcgtgccgcc cgcatcggac ggggatatgc ccggcgaggt
      421 gaaagagcgg tcgcatgacg gcggaaaacc caggcactgg cgtcgcactc cccgcacgga
      481 gcatgtggcg atcgagctgc ccgagcccgg gcagagaccg cggcgtctga cggttcccga
      541 cagcggcggg ctgaagttgg tggtgacggt gcgcgaagcc ggaacgtccg tgcttcccgc
      601 cggtacccgt gcggtctctg tatttctggt caattaccgt acaccggacg cgttgcagcc
      661 atatcgcgcc tttatcttcc aagcgggcat ccggctggag tgtcgggaag cctttgtccc
      721 gcgccccgat ccgcgcggct gggagcctga cgaagagtgg gacgagcgcg ttgccgacct
      781 ccagtaccgc gactcggtcg aacatgtggt cggccatggc gtctctgggc atgccgaggt
      841 cggtgacgac ggcgcttgcc gtgttgtgga gtcggtctgg attccccagg gtgaggtcga
      901 gcgcattgcc ccgtccagca ttccggacgt ggaactgggt atggaagttt tggcggaatt
      961 ggccgatggt gccgacgcca aggccaaact cggcaagctc gtcacgcgct acaaggagtg
     1021 gatcgggcgg cagcgctccg gtctttcggg gctcaatgcg aaccggacgc agacggccaa
     1081 cgacatgctg gtggaagcgg cgaacagtgc cgaccgcatt gcggcgggga ttgacctgct
     1141 ggacgatcct gacgtcctga cggcgttccg cctggcgaac aaggcggtgg cacggtcatt
     1201 gcgccagcgc ttcgggcaga ttcagggcaa agcgcccaag gcggttgacg cgccccggtg
     1261 gcgtccgttc cagttggcgt acctgctgat gacgatgcgc ggtgtcgtga atccgaagca
     1321 tggcgaccgc gatgtggtgg acttgatctt cttccccacc ggcggcggca agacggaggc
     1381 ctatctcgcg ctggccgcgt tcaccctcgt gctgcgccgc ctcagatcgc gggacgccaa
     1441 cggcaaaccg accatccaag gcgcgggcgt gagtgtcctc atgcggtaca cgctgcgtct
     1501 gctgacgctt gaccagctcg gccgcgcggc gtcgctcatc tgtgcgctcg aactggaacg
     1561 gcaggcccat cccgagctgc tcggcgagtg gccgttcgag atcgggctct gggtggggca
     1621 ggcggccacg cccaaccgca tggggcaccg caacgaccgc agtgcgcagg cgccctatac
     1681 ggcatacaca aaaaccaaac agtttagcag agacgacagc aagccgtcgc cgattccgct
     1741 tgaagagtgt ccctggtgcg gcaccaagtt cacgcgcaac tccttccggc tggtgcccaa
     1801 tgaaaacaag ccgttggatc tgcggattca ctgcaccaat gagcattgcg agttctgtgg
     1861 cgaccgctat ttgccggttg tggcggtgga tgagccgatc taccggcgac tgccctgctt
     1921 catgattgcc accgtcgaca aattcgcggc gctgccgtgg acggggcaga ccgggacgct
     1981 gttcggcaag gtcagccgct ttgacaagga tgggttctac gggccatgcg atcccggcaa
     2041 aggggtgccg ctgccaggcg gatgccttcc cccgccggac ctcatcatcc aagacgagct
     2101 gcacctgatc tccggtcctc tcggaacggt tgccgggctg tacgagacgg ccatcgacgc
     2161 gctggcgacc actgccttgg acgagatgtc gatacgcccc aaggttgtgg ccagcaccgc
     2221 gacggtgcgc cgtgcagaca gtcaaatccg ggcgctgttc aatcgcagcc gcgtcagcgt
     2281 gtttccgccg ccggggcctg acagacgcga ctcattcttt gctcacacac agcatgcgca
     2341 cgaagagccg ccgcgtctgt acatcggtat cgccgcccag ggacgcagct tgaaagtggt
     2401 gttgctgcgg gcaggcctgg ccttgctgag tgccggccag accgcctggg atgaagccgg
     2461 ggacaattca gatcaaaacc ctgccgatcc ctacatgacg ctcctcgggt actttaactc
     2521 gctgcgggaa cttgggggca gccgccgcat catcgaagat gaactgcggt cgcggctggc
     2581 ggagtatggg cagcggcggc gtatccaacc tgtggaccgg ctgttcagca accgcatcat
     2641 cgcttacgag gtgctcgagt tgacctcgcg tgtgcccacc aacgatgtgg cggcggccaa
     2701 gcgccgtctg gcactgccct ttgccagcga cgagcgcgtg gatgtcgccc ttgccaccaa
     2761 catgatctcg gtgggcttgg acatcacccg gctcgggctg atggtggtgc tcggccagcc
     2821 caagaccagt tcggagtaca ttcaggcgac aagccgcgtc ggacgcgact caaagcgtcc
     2881 ggggttggtg gtgaccttgc tcaatatcca taagccgcgc gaccgttccc actatgagcg
     2941 ttttgcagtc taccacagcc acttctatcg gaatgttgaa gcgaccagcg tcactccgtt
     3001 ctccccgagg gcgctggatc gcgcactgcc tgcggtgctc gtggcgctgt gtcgccagat
     3061 catccccgat ctcacgccag cccgggcggc aaaggatatc ctgacgctgc gcgatcgtct
     3121 ggggccgtta tgccaactga tcgcggcccg tgcggagaat cacaatggcg acttgagccc
     3181 tgcggatcgc acggcgctgg cagccaaagt gctgaaccgc tgcaacagcc ttctggacga
     3241 ttggctgtgg attgccaaac aggcgcagga cgagggctct gggttgcaat atcaacggga
     3301 ggagacatca ccaccgcgac gactcttgta tgactttctt gatccggagg tgccgaactt
     3361 ggctccagcc caacggcgat tccgcgccaa ccgctcgatg cgggatgtcg aagcctcggt
     3421 ggatattcga gttaaaaacc tcaatgactg gggagccaaa ccatgaagaa cccccagata
     3481 cgttccagcc agcttatcac gacctttggc cccggtgcga tggtcgatct gccgaaagac
     3541 gctgtgatca tcgcgggcct ggataactgg cattacagta ggcggttacc gttgccgctg
     3601 gtctccgagc cacggctgca gcgaaagctt gccgatatca tcgggttgcc cactgtggcc
     3661 ctgcgtaagc cgccgccgcc catcgaggaa cgggggttca cgcccgacat catcggctat
     3721 cgcttcccgg agtggttcat tgttcaaaag gccgtgatcc ggaacagggg cggacggatc
     3781 tgccgtgccc gccggctagt taatctcaag gatctggatg ggggccgcta ccgggaccgg
     3841 gacggcaaga agtacccggt ggttccggtg cgttttgtac gcgcctgcag gcgggggcac
     3901 gtcggcgaca tcgactggtg gacctttgtc cacggcgatg accgggactg ccgccgtgga
     3961 ttgtacatgg aggagcgtgg caccagcggt gacctcgact cggtatggat cacctgcgaa
     4021 tgcggtgcgg aacggccgat gagccatgcc gcgcgaatgg aattgaaggc gctcgggcat
     4081 tgcaatggat cccgcccgtg gctggggccg ggctcgcgtg aggcgtgcgg tgaccagaat
     4141 cgcctgttga tccgcagcgc cagtaacgct tattttccgc agttgctctc cgtgatctca
     4201 atcccggatt cgctgacggc ggtggatcag gcagtcgcgg cactgtggga gtcacacttc
     4261 tcggcggtag cgacagaagc ccaattggct tttgtcatga cgatgcctgc cgtcaaacag
     4321 caactcgatg gatttacgcc ggagcaggtc tggacggtta tcgagcgacg caggaggagc
     4381 ggcgacgagg atgaacgccc gattaaggag gttgagtttg aggtgctggc acaggcaaag
     4441 gacgagatcg gagaagacaa gccggaaggc gacttctatg cccggacgtt gccgagtgcg
     4501 gtatgggcgg atgcctggac ggatgtgatt gagcgcgttg tgctcgtcca ccggttgcgg
     4561 gaggttgtgg ctcagcttgg gtttacacgg ttcgatgcag ccgggcccga catcacaggt
     4621 gaactggaca ttgatgttca gcgcgcgcca ttggcgatag actgctcttg gctcccgacc
     4681 atagagaacc gtggagaggg ggtgttcatc cagttcaagc cggaggccat agcggcatgg
     4741 ctggatcagc ccgccgttca gcaacgggac aaggcgcttc aaagcggctt cgatcaatgg
     4801 ctgggcgagc acgaaaacag taaacacaag tatcccgggc tgccttacta catgttgcat
     4861 tcattctccc acctactgtt gatcgcgatg tcactggagt gcgggtatcc ggcctcatcc
     4921 atccgggaac gggtatacgc gatgcccagt cagtatgggg tgttggtcta caccggctct
     4981 ccggatgccg aagggacgct cggcggtctt gtggaagcgg gacgccggat caaattccac
     5041 atcggacggg cgctggacat ggggatgctc tgttcaaacg acccggtgtg tgcattccat
     5101 tctccgcgct cgcaggacca ccagcccctg ctgggcagcg cgtgccacgg atgtctgttg
     5161 atttcggaga catcctgcga acagcgcaac aacttcctcg accgtgcctt ggtggtcccc
     5221 accgtagaac agcttggcac cgaatttttc agaacgatac cccatgcgtg atgtcttcca
     5281 taagctcacc tccggcagcc tctgccagtt ggccgcttcg ctgcggggtg gcgagttgtc
     5341 cagaggtatc acgacccatg ccgtaaggca gatcgcgggc ggggttttgg tcggggattt
     5401 ggccattgca ctgaccgcct tgcgtgagga tggctggttg ccatctcaga ttgccgagtt
     5461 ggccgagact gtcgtcgaga gccgggcgcg ggtggcacaa ccggaagagt tgttcgacct
     5521 tgtcctgtca ggtccggacg tttcaggagt gccgacacgc gaaaccgctg ccgtcataca
     5581 tgcgctgatc gaggaggcgc aacgagaggt catcgtggtt ggatacgtta ttcataatgg
     5641 caagaaactc tttgaacggc ttgcggaaaa agccggagag acgccgggac tggatctctg
     5701 gctctgcttc aatatccagc gtccctacaa agacacgtca ctctcctccg agatcgtgcg
     5761 ccggttcgcc aataagtttg cgaaagacga atggccgtgg cgaccgttgc ctcccgtcta
     5821 ttacgatccc cgtttactgg aagacgggac gacgcagagc agtctgcatg cgaagtgtct
     5881 gatcatcgac cgcaaggcgg ctctcatcac ttccgccaac ttcaccgagg ccgcacataa
     5941 acgaaatatc gaggccgggg tgattgtccg ctacgagcct ttggtcatac ggattgccac
     6001 ctatttcgat acgcttcgcc agacgctgtt cgcccccgtg gacttctcat gtcatcaagg
     6061 gtgcccatga ccatctcggt tgccatctgt aaaacgacca gtggcgatga cataccacag
     6121 tgaatccgct ggacgttcag gtgcgaaaaa gctaaactgc gttttagtta gagttgtttc
     6181 ggtccggagg ccggataggg gatgtggcac atattcagga ggcgcggatg acggcaagag
     6241 atgtgttctg gatgacgggc aacgcgatat gttgtgtggt cttgcacact gttgccgcgg
     6301 acggcagttg gacgaaccgg gtcggcggga agtggaatga gcccggcaat tggttgggtg
     6361 gcgttgtcgc ggaaggggag ggcaacacag cgttcttcac gaacgcgccg tccgccaaat
     6421 gcctcgtcag ggtgccgtct gccggagtca cgctgcagaa tctgctgttt tccggattct
     6481 caaacgcctt ctatttgttt gacgggttcg tcacgctgga ggggggaaac cctcaagtcg
     6541 gagtcacgaa cccgaatgac agcaacaatg ccaataatta caacacagac ctccgcggca
     6601 cgaacggggt gtcgttttac ggcggcacct tgggcatgga cacgtcgtat acggggccga
     6661 cccgtctgtg cggcaaaaac aaaacgctca acaccttcat gcacttctat gtggccgagc
     6721 gtggcgtggc ccccagcaac tttctctccg gcgggacgct gatctttgac ggcggtcatt
     6781 tgcggctgga ggggtgcaag gctaaggcgg gtggcacagg cgtgttcact ctgacggagg
     6841 gcgacaccct gatccactgg aaatcgggga caacgccgtg ggcgggattt ccggtctccg
     6901 gaaatggggt tcccgacgga gcgttctgca ggcaacagat ccatgatcag gcctatatac
     6961 tcagccaccc cgccacggca tccggtgatt tctctctctc gatcgtcagg aacagcggca
     7021 gtctcgccta tcagtggttt gatcagatcg acatgcaggc atacgggatc ctcaccatcg
     7081 tggtgagcgg gtatgagacg cagatcgaaa ccgacaccat cgccggtcgg gaacggtttt
     7141 tccggatcat cgggagcggg aacaacagta ccaatcttgc gatctttcgc atgcggggag
     7201 cgaacgggtt ccccggagcg atcgaggctt ccgcagtcaa ggtcgaggcg ctggcgcaga
     7261 ccaacggtgt gaaccccgaa attcccgacc tgactgttgc cgccaccacg atcctgacgg
     7321 tgccggagcc ggaggcagtc ctgtccgtgc cgggtgtgac ccttatcgaa ggagagttgg
     7381 cgaaagaggg tgcggggact ctggcgctgc agcacgttta cggggccgat ccccgtgtgt
     7441 gtgtccgcgg tggcacgctg gcgctcgctg ctccagtggt tccggagcct gcgccggtcg
     7501 gcggggcatg gttccacgcg gatgcgagtg caacggccag cctggtgacc gaacaggaga
     7561 acgggacgaa tttcgtggtc tgctggcggg atgtccgggg caacggcatc tacgccacga
     7621 acgatgtcgc caacagcggg ccgcgtccgt ttctccggtc cgcgtgccag aacggattgc
     7681 cggtcgtgga tttcggttcg taccactatc cggcgaaagg ggtggtgggt tatggcgcgt
     7741 atctgaactg gtccgagatg tggacgaaga tttacgagtg ttatttcgtt tacagcgata
     7801 ccgaggacac ggcctcgctg tccgccggga cgtactggcc cacgccggtc gggaactcga
     7861 atcataatgc ttttttcgcg cggggggtca acggggcgat tctgggcggt ccgctggcac
     7921 aggaagaggt cttgtccgga gtgatctcgc tggatcatgc aactgtgcct gcggcaaccg
     7981 tcgtgccttc cggctttcat gtggtgtcgt ttgtcgccac gaacgatgtc cgggccggcg
     8041 cgttcgcgcg tgagcgggtg aacagttatg gcggagtccg gatcgcggag gtgctggtgt
     8101 tcacgaaccg cctcacggtc gcggcgcggc gggagattac ggactacctc agccgcaaat
     8161 ggttcggccg cgagccgggg atcggggcgc tgacattgga taacggaagc gcggtgcggg
     8221 ttgcggacgg cgagaccgtg cgcgtcggcg cgctatgtgt cgggaagcca gccgtgaaaa
     8281 cgggcggcgg ggtgttgtcg gcggagacgg tgagcggcga ttctctggtg atttcgaacg
     8341 ggaccgtgcg tcagacgggc gcggccggtc ttttgtccgg aggcgtcagc tgtgtgggtt
     8401 ccgcaacatt ggatgtggcc gatgaagcgg gatcggttac cgccggatgg atcgatgcgg
     8461 agggggaact gtctaaggcc ggcgagggtg aactgacggt cgcgacgctg ggtgtgggtg
     8521 tgacgtccct gcatgtgcgg ggaggagccc tcaacgttac gggcgtggac cccgcgtacg
     8581 gtcaggatgc ggtctttcat gtggatgcgg acgatgccgc cagtttggag acggttgcgg
     8641 tgaacgggac gaacttcgtg acacgctgga acgattgccg gaccaacggg ttctacgccg
     8701 cggcgcagga cacggtccac cggcccttcc tgagccccgg gcagcagaac ggacgcagtg
     8761 tggtggattt cggcgcgttc accaaagaca cgaatgcggt ttacacggcg tggctcaact
     8821 ggaaggagta caaacattcg gtgcgcgacg tgttcgtggt ggtccgggac accgaccctt
     8881 ccaagcaggc cacgctggtc ggcaaccaga accactatct ggatttcatg cggggcgctt
     8941 ccggcgcgct ggtgggcgag catccgcact cggagccgat ccgcaacggc tcctgcgcga
     9001 tcgacggcac ctccgtgacg tatacggcga ggttgccgga cggattccat gtggtgtcgt
     9061 tcttcccgac caatacggtt cgcgcatcgg cgttcgggcg cgaacggttc aacgggttcg
     9121 gcggacatca actggcggag gcgctctttt tcacccgcga gttgtcggcg aatgagcggc
     9181 agaacattca cgctcaactg ctctccaaat ggctggggca gacattgcat cgcgcgttcg
     9241 accatatcct cgtcgagaac gggggcgtat gctcgatcac gaacatgaca ctcgccacag
     9301 acctgctgga gggaacagga acaatccggg cgcaggggct tacggcggac gcgctttcgg
     9361 cgggcacaac ggaaacggca atcgggagcc tggcggtttc cggcggactg acgctgccgg
     9421 acggggcggt tgtggatgtg gattgcaacg cctttgcctg cgaccgggtg gatgtgcagg
     9481 gcacgctgac tatcggaggc ggcggcaccg tgcggttgcg gggtgacggg ggcgacatag
     9541 gggggaaagt gttcaccctg ttctcgttcg acacttgtgc ggggggcgag aatctgacgt
     9601 tgtggcaggt cacgggcgag atcacggcgc ggtatgtcgt gcggttgtgg atcgaggata
     9661 accagattcg cctcggggtc aagggaaaag ggacgcaggt tctgttgcgt tagtttttca
     9721 ccttgtcgat gacaaggcaa aaacgaaaag gatgggatag gtgtggtccg gcgacaaaga
     9781 tgcggacggt ttttccaagg agccgtgctc agcgcacatg tatacgtttg ccggtcggga
     9841 tgcggcaggc gctggcacgc ccgaggatgc gggcgcgtga ttccggaaag ggagcgggcg
//
LOCUS       DAOSRB010000102         9863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6633_length_9863_cov_7.025285, whole genome shotgun sequence.
ACCESSION   DAOSRB010000102 DAOSRB010000000
VERSION     DAOSRB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6633_length_9863_cov_7.025285"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1504
                     /locus_tag="PLZ74_06825"
     CDS             272..1504
                     /locus_tag="PLZ74_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98071.1"
                     /translation="MSFEMSRKGFLGMGGSFFAANVFSATAQKDKDGKVIAGFDETDA
                     GKIAQVKWEPFSDKKVRVGIAGHGICKFGCSFGYQNHPNVEIVACTDLDPARCADMQK
                     QLKAPRTFKSCEEMIKEMRGKMDAVYIATDPPSHAPLAILAMENGMHAVSAVPAFWGD
                     IQEEYAQKLVDTAKKTGMIYQMNETTAFRPQCFAMRALYKGGALGEIIYTEGEYWHHG
                     SRPMGSYKGWRNFAAPQWYCTHGNGFYNCVTHGHFTKCACLGVPTHYDAKKSFIGKPG
                     NYGNIYGTEVALLKTSEGGMARMGRGGDTPSAIQETGRCLGTRGQYDEGYKGEATKEE
                     LAKIAYNKPPLPPGVPAGGHGGSHGYLTDDFIRAILLGQKPVVDVICAVNMTIAGIYM
                     HRSAQKDGEWLRIPHFAI"
     gene            1635..4445
                     /gene="ppdK"
                     /locus_tag="PLZ74_06830"
     CDS             1635..4445
                     /gene="ppdK"
                     /locus_tag="PLZ74_06830"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOR98072.1"
                     /translation="MKTSRARKSAGKTAKFVYSFGSGRSEGSVSMKTLLGGKGANLAD
                     MSGLGLPVPPGFTITTEACAKYYEVGEKALHALLRDQVTEALRKLEKATGKTFGHGPN
                     PLLVSVRSGAAASMPGMMDTVLNLGLNPKTVEAMIERTGNPRFVWDSYRRFIQMFGNV
                     VMGVEHHEFERELENVKHARGYTLDTELMTEDLQDVVARYMKRVREIKGCAFPTDAME
                     QLWRGIDAVFGSWNNPRAIKYRALNDIRGLLGTAVNVQTMVFGNSGSNSATGVAFTRD
                     PSTGEHKYFYGEYLINAQGEDVVAGIRTPQQITIRGSREWAKARNITEAVRKVQYPAL
                     EEAMPKVFKQLDAIRVKLERHYRDMQDLEFTIEDGKLFILQTRSGKRTAAAAVKIATD
                     LVREKIIDEKTAVLRVEPGQLDQLLHPVFVRSAEAKAKKITVGLPASPGAATGQIVFN
                     AATAELWAREGKKVILCRIETSPEDIGGMNVAAGILTARGGMTSHAAVVARGMGKCCV
                     SGCGELHISYDKKTISCNKLVLKEGDWISLNGSTGLVYEGQIETEDPKLTGPFAMIMA
                     LADKYRKLGVRTNADTPRDTAKAVAFGAEGVGLCRTEHMFFEGDRVISFRRMILVAET
                     VKSLRQALAECTDPEERTRLEQELAAPLKQYRQALKELLPLQRKDFISMFKALKGRPC
                     TVRLLDPPLHEFLPHDEAGQAEMAREMDLEVIQIREVVESLHETNPMLGHRGCRLGIS
                     YPEVTEMQARAICEAAAAVKGSQPEIMVPLVGNVKELTAQKELIHKVLADVERTRKVK
                     LPIRIGTMIEVPRAAVTADQIAAEADFFSFGTNDLTQMTCAFSRDDAGKFLGEYVARG
                     FYDYDPFQVLDQEGVGRMVEIAMQYGRGVKPNLKVGVCGEHGGDPKTVEFCHKLGFSY
                     VSCSPFRVPIARLAAAQAALRS"
     gene            complement(4531..5838)
                     /locus_tag="PLZ74_06835"
     CDS             complement(4531..5838)
                     /locus_tag="PLZ74_06835"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR98073.1"
                     /translation="MTGTKNKMIGLAVCAVMAGGALCAVRAQTESRPRVLIIGDSISL
                     GYTPFVKAALSNRMEIVHAPGNNQATATGLAHLETWLGTGKWDVIHFNWGLHDLKYID
                     DQGKATDVAKGRQWVPVEQYETNLGTLVRRLKTTGARLIWCSTTPVPEGTAIRIAGSE
                     VAYNEAALRVMRGAGVPVNDLHAFVGPPERRLAMGGRPRDVHYTAAGSKALAGEVVRA
                     IETALESRAGQDGWVELFDGKSLAGWRPYGKPVGTAPGEGWSVEGGLLRKKPKIRGGD
                     LITEKTYGDFELEWEWRISPGGNNGVKYCVTEARPGAPGYEYQMIDDLSAKWVKLPAN
                     ARTASFYAVLPPAADKPLKPAGEWNVSRIVVRGDRCEHSLNGRKVLEYVFGSAEVKAG
                     VAASKFAEVPDFGDKIKGHIMLTDHTDETWFRSIRIREPAAQP"
     gene            6148..7119
                     /locus_tag="PLZ74_06840"
     CDS             6148..7119
                     /locus_tag="PLZ74_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR98074.1"
                     /translation="MIRKSLITGGAGFIGSHLARRLLAAGHEVTVIDNLFTGTKANIY
                     DLLDNRRFTFILHDVTQPFWGQFDEVYNLACPASPIHYQRNPVETLKTSFLGIMNVLE
                     LALKTKARVFHTSTSEIYGDPEIHPQPESYWGNVNSIGARSCYDEGKRAAETLCADYV
                     HEYGVDVRMIRIFNTYGPNMHPQDGRVISNFIMQALRNQDITLYGDGSQTRSFQYVDD
                     LIDAILRFMALDHKKVKTFFEGRAMRVPVINIGNPGEYTIRQLAEETLRLLPKSRSRL
                     AFHPLPKDDPRRRKPDITLAGELLGWAPSVTLPEGLAKTIAYFQNLV"
     gene            7142..8398
                     /locus_tag="PLZ74_06845"
     CDS             7142..8398
                     /locus_tag="PLZ74_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98075.1"
                     /translation="MPFPQAVLPLLNLVCGLALGAALSVAAQTEQPPPPSWGEIGRVN
                     MRGGLRIFWNVGGKDHKANEREALARGFEPVDLLNTYSDYPGKQREKIIPNGKNPWAK
                     PPQFERIIRRNIAMKQGRGLFVHDIEFDFDEEPEALWTNPVLRAASGADTVEAFRKAY
                     YREWGTWYTLPCRWAKEAFPHLPVGIYGPQPFRRDYWGICGKDAQQIDGTHKTDAELW
                     QHIDPAVDFCIASLYCFYDRPDSVFYMGANVEENAQRMALTGDKPLYAYLWLRYHNSN
                     KKLGTREVDDYLAESMAILPFFCGARGVVLWGWEPKSTGQPYRTLPLFMKSLERVAGV
                     SEELGRARPSHDEPVHAAWKARRPLIRRLRVTEDVWLVLATDPWQEEGAERTLRVSCG
                     TRAFDLAVRGKHTEIYRIDGGNAERR"
     gene            8722..>9863
                     /locus_tag="PLZ74_06850"
     CDS             8722..>9863
                     /locus_tag="PLZ74_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOR98076.1"
                     /translation="MTGSILLILLLVFYLGGRFILVHMLRNAEKSIQMIGSEVENVVY
                     AEADNLRKRVDSLADAFAAAGESDAETLLQCQVTPQNGDAIAHFAALLNTDGSFRKGF
                     FRLPDGQAEPVGAEEMAPYLQTSAPLLNNRLTASLIPTGVIILRGKPILIAISPIEEG
                     GSGTQGFLLIGTLFSSHLVLDRIHQVTHGMQVAVHDKRVKPDSSDKRFPSAQPNGLAP
                     IFSGALNYHSGGFWHLGENAFEAVIPIHDILGRQVTSISIRLPQTFSSIASIALGWLT
                     VFVASVGIIFVLPMFWLQTRIVLNPLSLLTDQIRKIGERPLDNHTLAIDWPRKDEFGM
                     LAQSVNGMLEALARKSRQVGQIEQQQRALIAGMPDGLCVFDKEARL"
BASE COUNT         2014 a         3048 c         2978 g         1823 t
ORIGIN      
        1 aatggaaccc tgaaacgcat gaccttcgat cgcacccctg tcacgaaccg attaccctga
       61 ccgttgaggt ttctggttac aggttttcgg cagatgatgt atgatatctt gtaaatgttg
      121 aaacaacgaa tgcgggcaga gcccgcgacc tcacttcgta tcacacaaag actcaaagga
      181 cacaacggtt ttttcatgat cccttcatgt gagaaacagc ttgttttttt attggcctta
      241 tcggtcgata aggcaccaaa ggaggatgga tatgtctttt gaaatgtccc ggaaagggtt
      301 ccttggtatg ggtggttcgt ttttcgccgc gaacgtattc agcgcaacgg cgcagaagga
      361 caaggacggc aaggtcatcg cgggtttcga tgagacggac gcaggcaaaa tcgcgcaggt
      421 caagtgggaa ccgttctccg acaagaaggt gcgcgtcggc atcgccgggc acggcatttg
      481 caaattcggc tgctcgttcg gctaccagaa ccatccgaac gtcgagatcg tcgcgtgtac
      541 ggatctcgat cccgcgcgtt gcgccgacat gcagaagcaa ctcaaggcgc cgcggacttt
      601 caagagttgc gaggagatga tcaaggagat gcgcggcaag atggacgcgg tctacatcgc
      661 gaccgatccg ccgagccacg cgccgcttgc gatcctggca atggagaacg gcatgcacgc
      721 ggtctcggct gttcccgcat tctggggcga cattcaggag gagtacgcgc agaagctcgt
      781 cgataccgcc aagaaaacgg ggatgatcta ccagatgaac gagacgaccg cgttccgtcc
      841 ccagtgtttt gcgatgcgcg cgctctacaa gggcggcgcg ttgggcgaaa tcatctatac
      901 ggaaggcgag tactggcatc atggctcaag accgatgggc agttacaaag gctggcgcaa
      961 tttcgccgca ccgcagtggt actgcaccca cggcaacggc ttctacaact gtgtcaccca
     1021 cggacacttc acgaaatgcg catgtctggg tgtgcccacg cactatgatg ccaagaagtc
     1081 gttcatcggc aagcccggca actacggcaa catctacggt acggaagtcg cgcttctcaa
     1141 gacatccgag gggggaatgg cccgcatggg acgcggcgga gatacgccgt ccgccatcca
     1201 agagacagga cgctgcctcg gcacacgcgg acaatacgac gagggttaca agggtgaggc
     1261 aacgaaggaa gagcttgcca agattgccta caataagccg ccgttgccgc cgggagtgcc
     1321 ggcaggcgga cacggaggca gccacggtta tcttacggat gacttcatcc gtgcgatcct
     1381 gctcggccag aagccggtcg ttgatgtcat ctgcgcggtc aacatgacca tcgccggaat
     1441 ctacatgcat cgctcggcgc agaaggacgg cgaatggctg aggattccgc acttcgccat
     1501 ctgactctgt aggccacagg caaaccgccc gaattggtgt gtcgcgcctg acgggattga
     1561 ctcccggggc gcgtgtggta cactgtctcc cgttttaaac caacaaggaa aaaaccgtga
     1621 aaaaacctgt ctctgtgaaa acgtcccgcg cgaggaagtc tgcggggaaa acggcaaaat
     1681 tcgtttattc gttcggttcg gggcgctccg agggttcggt ctccatgaag accctgctcg
     1741 gcggcaaagg cgcgaacctg gctgacatgt ccggcctggg tctgccagtg ccccccggtt
     1801 tcacaatcac cacggaagcc tgcgcgaagt actatgaggt cggcgagaag gctttgcacg
     1861 cgttgctccg cgatcaggtg acggaagcat tgcgcaaact cgagaaagcc accggcaaga
     1921 cgttcggcca cggccccaac ccgttgctgg tctccgtgcg ttcgggtgcg gcagcctcca
     1981 tgcccggcat gatggatacg gtcctcaatc tgggccttaa ccccaagacg gtcgaggcga
     2041 tgatcgagcg cacgggcaac ccgcgcttcg tctgggattc ctaccgccgc ttcattcaga
     2101 tgttcggcaa cgtggtcatg ggcgtggagc accacgagtt cgagcgggaa ctggagaacg
     2161 tgaaacacgc cagagggtac acgctcgaca cggaactcat gaccgaagac cttcaggatg
     2221 ttgtggcgcg gtatatgaaa cgggttcggg agatcaaagg gtgcgccttc ccgaccgacg
     2281 cgatggagca gctctggcgc ggcattgacg cggtgttcgg ttcctggaac aacccgcggg
     2341 ccatcaagta ccgggcgttg aacgacatcc gcgggcttct cggcacagcc gtgaacgtgc
     2401 aaaccatggt gttcggcaac tccggctcca actccgccac aggcgtggcc ttcacgcgtg
     2461 acccctcgac tggcgagcac aagtactttt acggcgaata cctgatcaac gcgcagggcg
     2521 aggatgtggt ggccggaatc cgcacgccgc agcagatcac gatccggggt tcgcgcgaat
     2581 gggccaaggc gcgcaacatc accgaggcgg tgcgcaaggt gcagtacccg gcgctggaag
     2641 aggccatgcc caaggtcttc aagcagcttg acgcgatccg tgtcaagctg gaacggcatt
     2701 accgtgacat gcaggacctc gaattcacga tcgaggacgg caagctgttc attctgcaga
     2761 cccgcagcgg gaaacgtacc gcggctgcgg cggtgaagat cgccaccgat ctcgtccggg
     2821 agaagatcat tgacgaaaaa acggcggtgc tgcgcgtcga accgggtcag cttgatcagc
     2881 tcctgcatcc ggtctttgtg cgcagtgccg aggccaaggc caaaaagatc accgtgggct
     2941 tgccggcctc gccgggggcg gccaccggtc agatcgtctt caacgcggcg accgccgaac
     3001 tgtgggcccg ggagggaaag aaggtcattc tctgccgtat cgagaccagc cccgaagata
     3061 tcggcggcat gaatgtcgcg gccggcatcc tgaccgcgcg cggcggcatg acctcgcatg
     3121 cggcggtggt ggcccgcggc atgggtaaat gctgcgtctc cggttgcggc gaactgcaca
     3181 tcagttacga caagaaaacc atctcctgca acaagctggt gctgaaggag ggcgactgga
     3241 tctcgctgaa cggctcgacc ggcctggtgt acgaaggcca gatcgagacc gaggatccga
     3301 agctcaccgg cccgttcgcg atgatcatgg cgctggcgga caaataccgc aagctgggcg
     3361 tgcggaccaa cgccgacacg ccgcgcgaca ccgccaaagc tgtggcgttc ggtgccgaag
     3421 gcgtcgggct ctgccgcacc gagcacatgt tcttcgaggg ggaccgggtg atcagtttcc
     3481 gccgtatgat tctggtggcc gaaaccgtca agagcctgcg gcaggcgctg gccgagtgta
     3541 cggacccgga agagcgtacc cgtctggaac aggagttggc tgcgccgctg aaacaatacc
     3601 ggcaggcgct caaagaactg ttgccgctgc aacgtaagga tttcatcagc atgttcaagg
     3661 cgctcaaagg gcgcccctgc acggtgcggt tgctggaccc gcccctccac gagttcctgc
     3721 cgcatgacga ggccggacag gccgaaatgg cgcgggagat ggaccttgag gtcatccaga
     3781 tccgcgaggt ggtcgagtcc ctgcatgaga ccaaccccat gctgggccac cgcggttgcc
     3841 gtctcggcat cagctatccg gaggtgaccg agatgcaggc gcgtgcgatc tgcgaagccg
     3901 cggctgcggt caaaggctcg cagcccgaga tcatggtgcc gctcgtcggt aacgtcaaag
     3961 agctgaccgc gcaaaaggaa ctgatccaca aggttctggc tgatgtggaa cggacccgga
     4021 aagtcaaact gccgatccgg atcggcacga tgatcgaggt gccgcgcgca gcagtcaccg
     4081 cggaccagat cgcggcggag gcggacttct tctctttcgg cacgaatgac ctgacgcaga
     4141 tgacctgcgc cttctcacgg gatgacgcgg gcaagttcct cggcgagtat gtcgcgcgcg
     4201 ggttctatga ttacgacccg ttccaagtgc tggaccagga gggcgtgggc cgtatggtcg
     4261 aaatcgcgat gcagtacggc cgcggcgtga agccgaatct aaaggtcggt gtctgcggtg
     4321 agcacggcgg cgatccgaag accgtggagt tttgccacaa actcgggttc agctatgtga
     4381 gctgctcgcc cttccgtgtg ccgatcgcgc gtctggctgc ggcacaggcc gcactgcgaa
     4441 gctgaggcgc gttccgctcc ggccgccctc atgcgcttcc gggcaaagag ggggtggccg
     4501 gtgacggaaa acgcggcctc gttctttggg ttacggttgt gcggcgggct cgcggatgcg
     4561 gatggatcgg aaccacgttt catccgtatg gtcggtcagc atgatgtgcc ccttgatctt
     4621 gtcgccgaaa tcgggaacct cggcaaactt gctggctgcc acgcccgcct tgacttcggc
     4681 gctgccgaag acgtactcca aaaccttccg gccgttgagg ctgtgctcgc accggtcgcc
     4741 ccgcacaacg atgcgggaga cattccactc ccccgccggt ttcagcggtt tgtcggccgc
     4801 aggcggcaac accgcataga acgaggcggt ccgcgcattc gccggcagtt tcacccattt
     4861 cgccgagaga tcgtcgatca tttggtactc gtagcccggc gcgccgggac gcgcttcggt
     4921 gacgcagtac ttcaccccgt tgtttccgcc gggagagatc cgccactccc actccagttc
     4981 aaaatccccg tacgttttct ccgtgatcag gtcgcctccc ctgatcttgg gttttttgcg
     5041 cagcagtccc ccctcaaccg accatccctc gccgggcgcc gttcccaccg gtttgccgta
     5101 cggccgccat ccggccaggc tcttgccatc gaaaagctca acccaaccat cctgtccggc
     5161 gcgggactcc agcgcggttt cgatcgccct gaccacttcg cccgccaatg ccttggaccc
     5221 tgcggcagtg taatgcacat cgcgggggcg ccctcccata gcgaggcgcc gttcgggcgg
     5281 accgacgaac gcgtgcagat cgttgaccgg cacccccgca ccccgcatga cgcgcagcgc
     5341 cgcttcgttg taggcgacct cactgcccgc gatacggatc gccgtcccct ccggaaccgg
     5401 tgtggtcgaa caccagatca accgtgcgcc ggttgttttg agccgccgca ccagcgtgcc
     5461 cagattcgtc tcgtactgtt ccaccggaac ccactgacgg cccttggcga catccgtagc
     5521 cttgccctga tcgtcgatat atttgaggtc gtgcagcccc cagttgaaat ggatcacatc
     5581 ccatttgccg gtaccgagcc acgtttccag atgcgcgagg cccgtggcgg tcgcctgatt
     5641 gttgcccggc gcgtgcacaa tttccatccg gttcgagagc gccgccttga cgaacggcgt
     5701 gtacccgagc gaaatcgagt cgccgatgat caatacgcgg ggccgcgact ccgtctgcgc
     5761 gcgcaccgcg cacagcgctc cccctgccat cacagcgcaa accgccaacc caatcatctt
     5821 gttttttgtc ccggtcatcc cgtccctccc gttcccgcac tggcgctgaa cgcattgaac
     5881 actccagcca gccccgtttc cacccgagcg gaaccccagg cgggcagcgt gtctcttttg
     5941 tgtggcggac agtgtagcac acccctcaac cgagcacaat tccttgttcg gaaacgggag
     6001 accggaatgc ccttgaaaaa gagggttgac tgttaccata cccccgccaa taataaagca
     6061 ctgcaacagg gtgggggcgt attttgttat ccggccggac gggatttccc ggctgaacgt
     6121 cacaaccccg aaacggaaac acgcatcatg atccgaaaat cactcatcac cggcggtgcc
     6181 ggattcatcg gctcccatct cgcgcggcgg ctgctggccg cagggcatga ggtcaccgtc
     6241 atcgacaacc tcttcacagg gaccaaggcc aacatctacg acctgctgga caaccgccgc
     6301 ttcaccttca tcctgcatga tgtgacgcag cccttctggg gacagttcga cgaggtgtac
     6361 aacctcgcct gtccggcctc gcccatccac taccagcgca atcccgtcga gaccctcaaa
     6421 acctcctttc tcggcatcat gaatgtgctg gagcttgcgc tcaagaccaa agcccgggta
     6481 ttccatacct ccacctcgga gatctacggc gacccggaaa tccatcccca accggaatcc
     6541 tactggggca acgtcaactc catcggtgca cggagttgtt acgacgaagg caagcgcgcc
     6601 gccgaaaccc tgtgcgccga ctacgtgcat gagtacggcg tggacgtgcg tatgatccgc
     6661 atcttcaaca cctacgggcc caacatgcat ccgcaggacg gccgcgtcat cagcaatttc
     6721 atcatgcagg cattgcggaa ccaggacatc acgctatacg gcgacggctc acagacccgc
     6781 agcttccagt atgtcgacga tctgatcgac gcgatcctgc gcttcatggc gctggaccac
     6841 aaaaaagtga aaactttctt cgaaggccgg gccatgcgcg tgccggtcat caacatcggc
     6901 aaccccggcg agtacaccat ccgccaactc gccgaggaga ccctgcggct tttacccaag
     6961 agccggagcc gactggcgtt ccatcccctg ccgaaggacg accctcggcg gcgcaaaccc
     7021 gacatcacgc tggcgggtga attgctcggc tgggcgccat cggtcaccct gcccgagggg
     7081 ctggccaaaa cgatcgctta tttccagaac ctcgtttgaa aagaaagggg gaaatcccgc
     7141 catgcccttc ccgcaagccg ttcttccgct tttaaatctc gtctgcggct tggccttggg
     7201 cgccgcgttg tccgttgcgg cgcaaacgga gcagcccccg cctccgagtt ggggagagat
     7261 tggccgcgtc aacatgcggg gcggcctgag gatcttctgg aacgtgggcg gcaaagacca
     7321 caaggccaac gagcgcgaag ctctggcgcg cgggttcgaa cccgtcgacc tgttgaacac
     7381 ctactctgac taccccggaa agcaacggga aaagatcatc ccgaacggga agaatccttg
     7441 ggccaagcca ccgcaattcg agcgcatcat ccgccgcaac atcgccatga aacagggacg
     7501 cggactgttt gtccacgaca tcgagtttga cttcgatgag gagccggagg ccctctggac
     7561 caatccggta ttgcgggccg catcgggcgc ggacacggtg gaggcgttcc gcaaggccta
     7621 ctaccgcgag tggggcacct ggtataccct gccttgccgc tgggccaaag aggcctttcc
     7681 gcaccttccg gtggggatct acggcccgca gcccttccgg cgcgactact ggggaatctg
     7741 cggcaaggac gcgcaacaga tcgacggcac gcacaaaacc gatgccgaat tgtggcagca
     7801 catcgatccg gcggtggatt tctgcatcgc gagcctctac tgtttttatg accgcccgga
     7861 ctcggtcttc tacatgggcg ccaacgtgga ggagaacgcg caacggatgg cgctgaccgg
     7921 agacaagcca ctctatgcct acctctggct ccgctaccac aacagcaaca aaaaactcgg
     7981 cacacgcgaa gtcgatgatt atctggccga gtccatggcc atcctgccct ttttctgcgg
     8041 ggcgcgcgga gtcgttttgt ggggctggga acccaaaagc acgggacagc cctaccgcac
     8101 gctgccgctt ttcatgaaga gtctggaacg ggtggccggc gtgtcggagg aactcggccg
     8161 cgcacgtccg tcccatgacg agccggtaca cgccgcctgg aaagcgcgac ggccgctgat
     8221 ccgcaggctg cgcgtcacgg aggatgtctg gctggtgctc gcgaccgatc cctggcagga
     8281 ggagggcgcg gaacgcacgc tccgcgtctc ctgcggcaca cgggcctttg atctcgcggt
     8341 ccgcggcaaa cacaccgaga tctaccggat cgacggcggg aacgcggaac gccgctgacg
     8401 cgcgggacgc gggagacata atcggttcgc gacgagggtg cgaaaggaaa acttgttttc
     8461 atcgcacctt ttcagctttt ttggcccgat ttatgcttca ctatatgatg ctttatcaac
     8521 attcccgcct cccgattttt ttgggaaacc cgagtggcaa ccggaggaaa gagagtgcgg
     8581 aacgcgttca caaacccggc gtcccttttt tgtctcattc tggaaaaggg acagacaaga
     8641 tacgattcgc tgttgcgcaa ccgtatgaag gcatccgcga tagtcaggga tttacggagc
     8701 atccgaacca agttcagtct ggtgacagga tccatcctgc tgatcctgct gctggttttc
     8761 tatctgggcg gacgtttcat tctcgtgcac atgcttcgca atgccgagaa aagcatccag
     8821 atgatcggtt ccgaggtgga gaatgtcgtt tacgccgagg ccgataacct gcggaagagg
     8881 gtggacagtc tagcggacgc gtttgcggcg gcgggagaat cggatgcgga gaccctgctg
     8941 cagtgtcagg tcacgccgca aaacggggat gcgatagcgc atttcgcggc gttgctgaat
     9001 acggacggga gtttccggaa agggtttttc cggttgccgg acggacaggc cgaaccggtc
     9061 ggggcagaag agatggcccc gtatctgcaa acgtcggctc cgctgctcaa caaccgcctc
     9121 accgcctcgc ttatcccgac cggcgtgatc atcctgcggg ggaagccgat tctcattgcc
     9181 atctcgccca tagaggaggg cgggagcgga acccaagggt ttctgctgat cggcaccctc
     9241 ttcagcagcc atctggttct tgaccgcatc caccaggtga cgcacggcat gcaggtggcc
     9301 gttcatgaca aacgtgtcaa accggactct tcggacaagc gctttccgtc ggcgcaaccc
     9361 aacggacttg cgccgatctt ctccggagcg ttgaactacc actcgggcgg attctggcat
     9421 ctgggcgaga acgcctttga ggccgtgatc ccgatccacg acatcctggg ccgacaggtc
     9481 acctccatct cgatccggtt gccgcaaacc ttctcctcga tcgccagcat cgcactggga
     9541 tggctcactg tgtttgtggc gtcggtgggc atcattttcg tgctgccaat gttctggctg
     9601 cagacgcgga tcgtgctgaa cccgctgtcc ttgctgaccg accagatccg aaagatcggc
     9661 gagcggcccc tcgacaacca caccctcgca atcgactggc cgcgcaaaga cgagttcggc
     9721 atgttggcgc agagtgtcaa cggcatgctg gaggccctcg cgcgcaagtc gcggcaggtc
     9781 gggcagatcg agcagcagca gcgagccctg atcgcgggca tgccggacgg cctgtgcgtg
     9841 ttcgacaagg aggcccgtct gat
//
LOCUS       DAOSRB010000103         9844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6646_length_9844_cov_5.658188, whole genome shotgun sequence.
ACCESSION   DAOSRB010000103 DAOSRB010000000
VERSION     DAOSRB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6646_length_9844_cov_5.658188"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1207
                     /locus_tag="PLZ74_06855"
     CDS             68..1207
                     /locus_tag="PLZ74_06855"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOR98077.1"
                     /translation="MGILTAGGDCPGLNAAIRAVAKSAMHYDIQVVGFLDGFRGLVEN
                     RYIQLEDRTVSGIITLGGTILGSSRDKPHKMNMGGTIMDMTDVAVANADRLNLDCLVC
                     LGGNGTQKNAYRLSQKGLNIITLPKTIDNDVVLTDTCFGYDTAKSIATEAIDRLHTTA
                     SSHHRLIAVEIMGHSAGWLALGAGIAGGADVILIPEIKYDIHAIADFINTRCHRGKRF
                     SIIAVAEGAIAIDDKPEDDDEEEDSDSKPQKKNGKKKNGDTNGSTPVKLPQKPLMIRE
                     SKVSRIVRELQQLTGIDARMTQLGHIQRGGTPTPADRLLCSRLGAMAGELINAREYNV
                     MVAVRGDDCVAVPLKDVAGHVRTVPRDHPWIKTARLLGTCMGDAS"
     gene            1355..3034
                     /gene="ettA"
                     /locus_tag="PLZ74_06860"
     CDS             1355..3034
                     /gene="ettA"
                     /locus_tag="PLZ74_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOR98078.1"
                     /translation="MAGEYAFSLINVTKQHENKIILEDVNLSFFHGAHIGVIGANGSG
                     KSSLLKILAGLDKEFMGECQVAKNTRVGYLAQEPELDDSKNVMQCVEEGVAGSKALLA
                     RYDEICEKLGEDMSDEESEKLNDELGRLQDIIDQNDLWSLDHQVEMAMEALRLPPPEA
                     PVAPLSGGERRRVALCRLLISNPDVLLLDEPTNHLDAESVAWLETYLKGFKGTLIVVT
                     HDRYFLNNVTEWILELDNGRTYPYKGNYEMWLVQKQEAMLRESKQAESRRKLIENELA
                     WVRTNPSGRRAKNQARVRRYEELIAGKVSTREDDLRIQIPAGPRLGDLVVRAEAVAMG
                     FDDRLLFEDMNFDLPRGGIVGVIGGNGAGKTTLFKLITGQLQPLSGTLTVGPTVQISY
                     VDQSRAELDPARTVYEEITGGSEYVSLAGEAMMNGRAYCSRFNFRGGEQQKLVGVLSG
                     GERNRVHLAKLLRSGGNLLLLDEPSNDLDVATLRSLEEGIADFGGCVMVISHDRWFLD
                     RIATHILAFEGDSVVTWFEGNYADYHEQRRKLLGEAADRPTRVRFRPLKHG"
     gene            3149..4543
                     /locus_tag="PLZ74_06865"
     CDS             3149..4543
                     /locus_tag="PLZ74_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98079.1"
                     /translation="MTTSRRSFLKRATFTGVPFLLPGRIWANAVKPNDKPGMAFIGMG
                     IQARALLNNFLRQDVNVLAVCDVDTTRREDARVKVDTFYKEHPEKGVPGNCKAYNDFR
                     EVLARKDISLVCVATPDHWHAYITIEAMRSGKDVYCEKPLTYSINEAVAVMEATRRYN
                     RILQTGAMQRSGFEFYTAAELVRNGCIGKITHVECSFGGPSRPHVDPQEDIPMEPGLD
                     WDMWCGGAPLVKYNDKLSPRGIHNFYPMVWRMDDLFGSGMCGDWGAHHLDIAQWGLGM
                     DAGGPVEVIRAERTEKDKANVALGGRAQSGVVLRFADGVTVKHTRLPIFGTVFHGTEG
                     TVSVNRGKFEMTHGKELISRFTKKEDGGSLEGALMKARKAFLTDSTYKVKLYRTKGGH
                     PADFVTCAKSRQRACSNEDVGGRAAILCHLCNLSYMRDASFRWDPVRNTFASGSGNPA
                     WLGREGGYRGGWSV"
     gene            4641..4796
                     /locus_tag="PLZ74_06870"
     CDS             4641..4796
                     /locus_tag="PLZ74_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HOR98080.1"
                     /translation="MSQHTSLKGSGKITVKRNVLKRFERVNLLKKRGQWKDGDRGLGL
                     PKTKPEV"
     gene            4917..5807
                     /locus_tag="PLZ74_06875"
     CDS             4917..5807
                     /locus_tag="PLZ74_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOR98081.1"
                     /translation="MSFGYLTVGIVVTLLAVAGSAAASDEPIRMRVIADRAPVCTATN
                     PTRETVLWLPSGAEVNLIGELVDGQEWVRVTAPPALALWIYRELVRDGRVIADKSQIR
                     TGPGQAFPVVAQLIRGEPVTVRGTYGDWLKIVPPAQQSFWMMRDNVEPLAFPAPEGES
                     DVADVPGGPASEGEVPVEAAPEVSLPAPAPIVERFRPPVPSELSAFVLDEKREQGRPA
                     ERRGILDWGGVGSVSTPFCLVGRRGAGEESAPVCHLILPVATYGPHIGAALSITGTEW
                     YVNGSALPVLIPTRVRMDIP"
     gene            6007..6999
                     /gene="amrS"
                     /locus_tag="PLZ74_06880"
     CDS             6007..6999
                     /gene="amrS"
                     /locus_tag="PLZ74_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOR98082.1"
                     /translation="MADVTQQRVRCGLCPKRCAIPPGHSGECRVRINADGVLRAVTYG
                     RPSAVHVDPMEKKPLFHFFPGASILSLGTVGCNFHCKNCQNAELSQGDPETMPAYDLQ
                     PAALPETARRQGCAHVAYTYNEPLVSYEYVRDCCRAARDAGLRNVLITAGYINEAPLR
                     ALAPLVDAVNVDIKAFQDEFYRNVCDGTLEPVLRSVLLFKEAGTHVELTNLLIPTLND
                     SDAFLDPLCEWIRDRVGAETPMHFSRFFPSFRMTRLPPTPIETLLHARDRAHAAGLRH
                     VYVGNAVVPEGENTRCAGCGETVVRRQRYVVTENRLTPQGACPACGRTAYGVWS"
     gene            7157..8698
                     /gene="amrB"
                     /locus_tag="PLZ74_06885"
     CDS             7157..8698
                     /gene="amrB"
                     /locus_tag="PLZ74_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOR98083.1"
                     /translation="MRRMLSGFCAMLLCAGAGCQRGGPVPEEAEMTKKVMASPLAGRW
                     YQDSERALRDEIATCCKDIMVVRKKSVCAAVVPHAGYRYSGRVAAGVYMRIDPSRLSR
                     VVVMGPSHYTAMRNQISVPDATHFATPLGELSADIGFLRRLRALPFVTHVPEAHTQEH
                     SDQIQLPLIQACLSKELPVVCMICGQFDAAGLLDAAAALRPLLDEGTLVVASSDFTHY
                     GSSYGYVPFTRDVDRNIETLDMGIFELFAQKDLQGFLERLRETGATVCGRDPLALLLA
                     MLPQEAKVERTGYDTSGRMTGDTRQSVSYVGAVVTGKWAPATPRAAPLAADSPIPRED
                     CDRLLALARDTLTRALRDGERRAAIAEPPKLTEGMLAVRGGFVTLNKRGALRGCIGEI
                     VPRREIWKVVREQALNAAFHDPRFNPLEATELAEIEIEISMLTPPKPVKSWREIVVGK
                     HGMVLSKEGHSAVFLPQVAPEQGWGIEETLTHLSRKAGLPSNAWREGATFLVFEAQVI
                     REKGR"
     gene            8705..9448
                     /locus_tag="PLZ74_06890"
     CDS             8705..9448
                     /locus_tag="PLZ74_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR98084.1"
                     /translation="MRSGDTMDLLTLEGVTKTFGDVRAVDGLTLSIRQGEILGFLGTN
                     GAGKTTTIKMLLGFFRPDSGKIAIWGGDPRTGAVRRRIGYMPETACYYPFLTIRELLD
                     FYGGLCGMTRADIRSRAEQLIGRVGLAGVEKRLLRHYSKGMLQRAGIAQALLHDPDLL
                     ILDEPFTGLDPLARIQLRDLIRELRAQGKTIFFSSHELSEAELICDRVAMIRRGQTVW
                     CGALHEVAGDGQQNLERIFLKMLGGEETA"
     gene            9445..>9844
                     /locus_tag="PLZ74_06895"
     CDS             9445..>9844
                     /locus_tag="PLZ74_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR98085.1"
                     /translation="MKMCGRQTGALVRVALLDLYRRKDLIVVWLLSLVLLLPLAFFSP
                     FGISGAGRYLNEIALLLVWLFSAVIGVGVAARLFPAEFESRTIFPLLAKPVTRGRIVL
                     GKYLGALAASVSALTVFYLAYGVLCGLRQSV"
BASE COUNT         1834 a         2689 c         3334 g         1987 t
ORIGIN      
        1 acccgcaaac ggacgtaaaa ctttgagcaa cgacaacaag agacaaaacg gtaaaacggc
       61 acgcagcatc ggcatcctga ccgcaggcgg cgactgcccg ggcctcaacg ccgcgatccg
      121 cgcggtggcc aaatcggcca tgcactacga cattcaggtt gtcggttttc tcgacggctt
      181 ccgcggcctc gttgaaaacc gctacatcca actcgaagac cgcactgtga gcggcatcat
      241 cacgctcggc ggcacgatcc tcggctcaag ccgtgacaag ccgcacaaaa tgaacatggg
      301 cggcaccatc atggacatga ccgacgtcgc cgtggccaac gccgacagac tcaacctcga
      361 ttgtctcgtg tgcctcggcg gcaacggcac acagaaaaac gcttaccgtc tctcgcaaaa
      421 gggcctcaat atcatcacgc tgccgaagac gatcgacaac gacgtcgtcc tgaccgacac
      481 ctgcttcggt tacgacaccg ccaagtcaat cgcgaccgag gcgatcgacc gccttcacac
      541 cactgccagc agccaccacc gccttatcgc tgttgagatc atgggccact ccgccggctg
      601 gctggcactc ggcgccggca tcgcgggcgg tgccgacgtt atcctgatcc cggagatcaa
      661 atacgacatc cacgccatcg ctgatttcat caatacccgt tgtcaccgcg gcaagcgttt
      721 ttccatcatc gccgtggctg aaggtgccat cgcgatcgac gacaaacccg aggatgatga
      781 tgaggaggag gacagcgact ctaagccgca gaaaaagaac ggcaagaaaa agaatggcga
      841 caccaacggc agcacccccg tgaagctgcc gcagaagcct ctcatgattc gtgagtcaaa
      901 agtcagccgt atcgtccgtg aactccagca actcaccggc atcgatgcgc gcatgaccca
      961 acttggccac atccagcgcg gcggcacgcc caccccggcc gaccgcttgc tctgcagtcg
     1021 gctcggcgca atggccggcg aactcatcaa cgcccgcgag tacaacgtca tggtcgctgt
     1081 gcgcggtgac gattgtgtcg ccgtgcccct caaggatgtc gccgggcatg tccgcaccgt
     1141 gccgcgggat catccctgga tcaagaccgc gcgcctcctc ggcacctgca tgggcgatgc
     1201 gtcttaactc gcgcccacta cgattgaacg aatgaccttc tccttgcctg caagcccatt
     1261 ggctggactt tgaggggggc ggttggtatg cttgccgaaa attcgcgcct attgtgaagg
     1321 ctggatgcag aggataagaa cgtaaggagt ccccatggcc ggcgaatatg cgttttcgtt
     1381 aattaacgtg accaagcagc atgagaataa gatcatcctt gaggatgtga acctctcgtt
     1441 tttccacggg gcgcacatcg gggtgatcgg cgcgaacggt tccggcaaat cttcgctgct
     1501 caagattttg gcgggtctgg acaaggagtt tatgggcgag tgccaggtcg ccaagaatac
     1561 gcgggtgggg tatttggcgc aggagcctga actggacgac tcgaagaacg tcatgcagtg
     1621 tgttgaagag ggggtcgcgg ggtcgaaggc tctcctcgcc cgttatgatg agatctgcga
     1681 aaagctgggc gaggacatga gtgacgagga gtcggagaaa ctcaacgacg agctgggccg
     1741 gctgcaggac atcattgatc agaacgatct ttggagtctc gaccaccaag tggagatggc
     1801 catggaggct ttgcggttgc cgcctcccga agcgccggtg gcaccgctct caggcggcga
     1861 gcgccgccgt gtggcgcttt gccgcctgct gatctccaat ccggatgtgc tgctgctgga
     1921 cgagccgacc aaccatctcg atgccgagtc cgtggcatgg ctggaaactt acctgaaggg
     1981 gttcaagggc acgctgatcg tggtgacgca cgaccgttac ttcctgaaca atgtgacgga
     2041 gtggatactg gagcttgata acgggcggac atatccctac aagggcaatt acgagatgtg
     2101 gctggtgcag aagcaggagg ccatgctgcg cgagtccaag caggctgaat cgcggcgcaa
     2161 gctgatcgag aacgaactgg cttgggtgcg caccaacccg tcaggacgcc gcgccaagaa
     2221 ccaggcccgt gtacgccgct acgaggaact gattgccgga aaggtgagca cgcgcgagga
     2281 tgacctgcgc atacagattc ccgctggacc gcggctgggc gatctggtgg tcagggcgga
     2341 ggcggtggcg atggggtttg acgaccggct gctttttgaa gacatgaact ttgatttgcc
     2401 gcgcggcggc atcgtggggg tgatcggcgg taacggcgcg ggcaaaacca cgctcttcaa
     2461 gctgatcacg ggccagttgc agccgctgtc cggaactttg acggtcgggc ctacggtgca
     2521 gatttcgtac gttgaccagt cgcgcgcgga actggacccg gccaggacgg tatatgagga
     2581 aatcacgggc ggctcggagt atgtcagtct ggcgggagag gcgatgatga acgggcgggc
     2641 ttattgcagc cgtttcaatt tcaggggcgg ggaacagcag aagctggtgg gggtgctgtc
     2701 cggcggcgag cgtaaccgtg tgcatctggc caaactgctg cgcagcggcg gcaatttgct
     2761 gctccttgac gaaccctcca atgatcttga tgtcgccacc ctgcgttcac tggaggaggg
     2821 catcgctgat ttcggcggat gcgtgatggt gatcagccac gaccgctggt ttctcgaccg
     2881 catcgccacg cacatcctcg cctttgaggg ggatagcgtg gtgacgtggt ttgaaggcaa
     2941 ttacgccgat taccacgaac agcgccgtaa acttctcggc gaggcggctg accgtcccac
     3001 ccgggtccgg ttccgtccgt tgaagcacgg gtgacgcgaa aacggcgcgt tggaaaccgc
     3061 ggaaacccgc ttgcctttcc gcaatcacgc atgatatcat tttcaaactt ttgtacggaa
     3121 aaacgttgat cgaaacaagg gggagaggat gacgacatca cgcagaagtt ttctcaagcg
     3181 ggcgacgttc acgggtgtcc cgtttttgct gccggggcgg atctgggcca atgcggtgaa
     3241 gcccaatgac aaacccggca tggccttcat cggcatggga attcaggcgc gcgcgctctt
     3301 gaataatttc ctgcgtcagg acgtgaatgt gctggcggtg tgtgatgtcg atacgacacg
     3361 ccgtgaggac gcgcgggtca aggtggatac gttttacaaa gagcatcccg aaaagggggt
     3421 gcccggcaac tgtaaggcgt acaacgattt ccgagaggtg ctcgcgcgca aggacattag
     3481 tcttgtctgc gtcgccacac cggaccactg gcacgcctac atcaccatcg aagccatgcg
     3541 ttcgggcaag gatgtgtatt gcgagaagcc gctgacctat tccatcaacg aggcggtggc
     3601 ggtcatggag gcgaccagac ggtacaaccg gatccttcag accggcgcga tgcagcgttc
     3661 cgggtttgag ttttacaccg ctgccgaact ggtgcgtaac gggtgcattg ggaaaatcac
     3721 ccatgtcgaa tgcagcttcg gcggcccgtc ccgtccgcat gtcgatccgc aggaggatat
     3781 cccgatggag ccggggttgg actgggatat gtggtgtggc ggtgcgccgc tcgtcaaata
     3841 caacgacaag ctgtcgccgc gcgggatcca caatttttac cccatggtct ggcgcatgga
     3901 cgacctgttc gggtccggga tgtgcggtga ctggggcgcg catcacctcg atatcgcgca
     3961 atgggggttg ggtatggatg cgggcggacc ggtcgaggtc atccgggccg aacgcacgga
     4021 gaaggataag gccaacgtgg cgctgggcgg ccgcgcgcaa tccggtgtgg tgctgcgttt
     4081 cgccgacggc gtgacggtca aacacactcg gctgcccatc ttcggcacgg tcttccacgg
     4141 caccgaaggc acggtgtcgg tgaaccgcgg caagtttgaa atgacgcacg gcaaggaact
     4201 gatcagccgc ttcaccaaaa aggaggatgg cgggtcactc gaaggtgcgc tgatgaaagc
     4261 ccgtaaggcc tttcttacgg acagcaccta taaagtgaag ttgtatcgga ctaagggcgg
     4321 acaccctgcc gacttcgtga cctgcgcgaa atcgcgtcag cgcgcgtgtt ccaatgagga
     4381 tgtcggcggg cgcgccgcga tcctgtgcca tctgtgcaac ctgtcgtaca tgcgcgacgc
     4441 gagtttccgc tgggatccgg tccggaacac ctttgcgtcc ggctcgggga atccggcttg
     4501 gctcggtcgc gagggcggat accgcggcgg ctggtcggtg tgacctggct ttttgcggtt
     4561 gatctacatc ctttttttag tattctactt cgttttctgc cttttacgga aggcgtttgt
     4621 tcggaggaga ggaagcagtc atgagtcagc ataccagttt aaagggttca ggcaagatta
     4681 ccgtgaagcg gaatgtgttg aagcgtttcg agcgcgtgaa tctgctcaag aagcgtggcc
     4741 agtggaaaga cggggatcgg ggcttggggt tgcccaagac caagccggaa gtgtagccgc
     4801 atcaccgatc gacgtttttt cgtgtcggtt tccgccgcat gcgcggcccg cttttgcggg
     4861 caatgcgcat gcggtttttt cggtttcggc atggtacggc ggaaggaggg gttgtcatgt
     4921 ctttcggata cctcacggtt gggatcgtcg tgaccctgtt ggcggtggcg gggtcggctg
     4981 ccgcgtcgga cgagccaatc cggatgcggg tgatcgctga ccgcgcaccg gtctgcacgg
     5041 cgacgaatcc aacacgcgaa acggtgttat ggctcccgtc cggtgccgag gtgaatctga
     5101 tcggagaact ggtcgacggg caggagtggg ttcgggtcac ggcgcccccc gccctcgcgt
     5161 tatggatcta tcgggagctg gtgcgggacg ggcgtgtgat tgcggacaag agccaaatcc
     5221 ggaccggacc ggggcaggct tttccggttg tggcgcaatt gatccggggg gagcccgtaa
     5281 cggtccgcgg cacgtacggt gactggctca aaatcgtgcc tcccgcgcaa cagtcttttt
     5341 ggatgatgcg cgataacgtg gagccgttgg ccttccccgc accggagggg gagtcggatg
     5401 tcgcggatgt gccggggggc cctgcttcgg agggggaggt gcctgttgaa gcggcgccgg
     5461 aagtttctct tcccgccccc gcgccgattg tggaacgttt tcgtcccccc gtgccgtctg
     5521 aactcagcgc attcgtgttg gatgagaaac gggagcaggg acgtccggcc gaacggcgcg
     5581 ggatcctcga ttggggtggc gtgggatctg tttcgacgcc gttctgtctg gtggggcgcc
     5641 gtggggccgg ggaagagtcg gcaccggttt gccacttgat tctgccggtc gccacatatg
     5701 gtccgcatat cggggccgcc ctttcgatta cgggaacgga gtggtacgtt aatgggtcgg
     5761 cactgccggt gctgattccg acccgggtcc gtatggacat cccgtagaca gaagacagga
     5821 ggcgggtgcg cgggcgtcct tgcccgctga tgggtcgtcg gatcggactg atcagttaga
     5881 tcattccttt gccaaacaac ccagacgtcg cagggcgaat gtcgcgggtc gcaagtcgac
     5941 gttcatggga ccgcttcact ttttcgcgga gatggggtat gctttatccc gtaagcaagg
     6001 tgtgtgatgg ctgatgtgac ccaacagcgt gtgcgatgtg gcctgtgtcc gaagcggtgc
     6061 gcgattcccc ccgggcatag cggggagtgt cgtgtccgga tcaatgcgga cggcgtcttg
     6121 cgggcggtga cctacgggcg tccgagcgcc gtgcatgttg atcccatgga gaaaaagccg
     6181 cttttccatt tttttccggg tgcgtcgatt ctttcgctgg gtacggtcgg atgcaatttc
     6241 cactgcaaga actgtcagaa cgcggagttg tcgcaaggtg atcccgagac gatgcccgca
     6301 tatgacctcc agcccgcggc gttgcccgaa accgcccgca gacaggggtg cgcgcatgtg
     6361 gcttacacct acaacgaacc gctggttagt tacgagtatg tccgcgactg ctgccgggct
     6421 gcgcgggatg ccggattgcg gaacgtgctg atcacggccg ggtacatcaa tgaggccccg
     6481 ttgcgcgcgc tggcaccgct cgtggacgcc gtgaatgtgg acatcaaagc ctttcaggat
     6541 gagttttacc gcaacgtgtg cgatggcaca ctggagccgg tgctccggag cgtgctgttg
     6601 tttaaagagg cgggcacgca tgtcgaactc accaatctgc tgattccgac cctcaatgac
     6661 agcgatgcgt ttctggatcc gctctgcgag tggatccgcg accgggtcgg cgcggagacc
     6721 cccatgcatt tctcccgttt cttcccgagt ttccgtatga cgcggttgcc tcccacgccg
     6781 atcgagacct tgctgcacgc ccgcgaccgc gcacacgctg cgggactccg gcatgtgtat
     6841 gtcggaaacg ccgtggtgcc cgagggggag aacacccgtt gcgcgggctg cggcgaaacg
     6901 gtggtgcggc ggcagcggta tgtggtgaca gagaaccgtc tgacgccgca gggggcttgt
     6961 cccgcatgcg gaaggacggc ttacggagtc tggtcatgac gcggccggga agagtgcgac
     7021 tccggtacgc gcgtcgggac ttcttgttcg gtgcggtcgc gatgctgggg tgtggatgcc
     7081 ggtggcgcgg cgcgcgagtt ctgccgttgc aggcctcgcg gatccgtcag atggctaaat
     7141 gggcggggtg atgcggatga gacgcatgtt gtctggtttc tgtgcgatgt tgttgtgcgc
     7201 gggcgcgggg tgtcagcgtg gcggcccggt gccggaggag gcggagatga cgaaaaaagt
     7261 gatggcctcg ccgttggcgg ggcgttggta tcaggacagt gagcgggcct tgcgggatga
     7321 gatcgccaca tgttgcaagg acatcatggt cgtgcgtaaa aaatcggtgt gcgcggcggt
     7381 ggtgccgcat gcgggatacc gctattccgg acgtgtggcg gcgggcgtgt atatgcggat
     7441 tgacccgtcc cggctgagcc gggtggtggt gatggggccg agccattata ccgcgatgcg
     7501 gaatcagatc agtgtgccgg atgcgacgca tttcgcgacc ccgctcggcg agttgtctgc
     7561 ggatatcggg ttcctccgtc ggcttcgcgc cctgcctttc gtgacgcatg tcccggaggc
     7621 ccatacgcag gagcacagcg atcagattca actgccgctc attcaggcgt gcctgtcgaa
     7681 ggaacttccg gtcgtgtgca tgatctgcgg ccagttcgat gcggccggcc tgctcgacgc
     7741 ggcggcggct ctgcgtccgt tgttggacga aggaacgctg gtggtggcga gttccgattt
     7801 cacccactat ggttcgagtt acggttatgt gcccttcacg cgcgatgtgg accgcaacat
     7861 tgaaacattg gatatgggga ttttcgaact gttcgcgcaa aaggacttgc aggggttttt
     7921 ggagcgattg cgtgagacgg gcgccacggt gtgcgggcgc gatccgctcg ccttgctgtt
     7981 ggccatgctg ccgcaggagg ccaaggtgga gcgtaccggg tatgacacct ccggcaggat
     8041 gacgggcgat acccgccaat cggtgagcta tgtgggggcg gtggtgactg gcaagtgggc
     8101 gccggccacg ccgcgcgcgg caccgcttgc ggcggacagt ccgattcctc gtgaggattg
     8161 cgaccgtctg ctggctttgg cgcgcgacac gctgacgcgg gcgctgcgag acggcgagcg
     8221 ccgtgcggcc attgcggagc cgccgaagtt gaccgagggg atgcttgcgg tacgcggagg
     8281 tttcgtgacg ctgaacaaac gcggcgcctt gcggggatgc atcggcgaga tcgtgccccg
     8341 gcgcgaaatc tggaaggtgg tacgcgagca ggcgttgaac gcggcgtttc atgatccacg
     8401 tttcaacccc ctcgaagcga cggagctggc cgagatcgag attgaaatct cgatgctcac
     8461 tccaccgaag ccggtgaaat cctggcggga gattgttgtc gggaagcacg gtatggtgtt
     8521 gagcaaggag ggccacagcg cggtgttcct gccgcaggtc gcgccggaac agggttgggg
     8581 gatcgaagag acgctgacgc atctttcccg aaaggcgggt ctgccgtcga atgcctggcg
     8641 cgaaggggct acgttccttg tgttcgaggc tcaggtgatt cgggaaaagg ggaggtaaaa
     8701 agtcatgcgg agtggagaca cgatggatct gctcacgctg gagggggtca cgaaaacgtt
     8761 cggtgacgtg cgtgccgtgg acgggctaac gctgtcgatc cggcagggcg agattctggg
     8821 atttctcggc acgaacggcg cggggaagac caccaccatc aaaatgctgc tcggcttctt
     8881 ccgtccggac tccggaaaaa tcgcgatttg gggcggtgac cccagaacgg gcgctgtccg
     8941 gcgacggatc ggttacatgc cggaaaccgc ctgttactac ccctttctga caatccggga
     9001 actgctggac ttctatggcg ggttgtgcgg catgacccgg gccgacatcc ggtcgcgcgc
     9061 ggaacaactg atcgggcggg tggggttggc cggggttgaa aaacgcctgt tgcggcacta
     9121 ctcaaaaggc atgttgcagc gtgcgggtat cgctcaggct ttgctgcatg atccggatct
     9181 gttgattctg gatgaaccgt tcaccggact tgacccgctg gcgaggattc aactccgcga
     9241 cctgatccgg gagttgcggg cgcagggcaa gacgatcttt ttctcttcgc atgaactctc
     9301 ggaggcggag ttgatctgtg accgggtggc gatgatccgg cgcggtcaga cggtctggtg
     9361 cggtgcgctg catgaggtgg cgggtgacgg acagcagaat ctggagcgta tctttctcaa
     9421 gatgcttggg ggggaggaga cggcatgaag atgtgcggtc gtcagacggg agcgctggtg
     9481 cgtgtggcgc tgttggattt gtaccggcgc aaggacctga tcgtggtctg gttgctcagt
     9541 ctggtgctcc ttttgccgct ggcgttcttt tcgccgttcg gtatctctgg tgcgggaaga
     9601 tatctgaatg agatcgcctt gctgctggtc tggctgtttt cggccgtgat cggggtgggt
     9661 gtggcggcgc gcctcttccc cgccgaattc gaaagccgga cgatctttcc gttgttggcc
     9721 aagccggtga cgcgtggcag gatcgtgctg gggaaatatc tgggcgcgtt ggctgcctcg
     9781 gtttcggcac tgacggtgtt ttatctggcg tacggagtgc tgtgcggtct gcgtcagagc
     9841 gtct
//
LOCUS       DAOSRB010000104         9829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6661_length_9829_cov_6.694496, whole genome shotgun sequence.
ACCESSION   DAOSRB010000104 DAOSRB010000000
VERSION     DAOSRB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6661_length_9829_cov_6.694496"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PLZ74_06900"
     CDS             <1..1007
                     /locus_tag="PLZ74_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98086.1"
                     /translation="RAVVKYFGPGKNLTDKVKVQAFHNAAFSGMPVAEYTLAGGNLAA
                     VTNTANVVTNAIICGLAPSAEAGDYYLRAYIDSNGNNVRDVWESWGYANFYGVSDKPF
                     DPRPVTVQFVPLAESPTVTILIEDADTDQDWFPDIYEYETNPGVGFLSKSGPSTGSDG
                     DPDFEINPNLGDTTGINTLFSALMLGSTDADGDEVGDLAELILGSDGQKVSTSGDGVS
                     DGLKIGLGLSPADTLALEMTGLELTGSEATLTWKLVVDQAEGVNRNLIKSLAGAPGPA
                     TYEVLFKPSLDAADWQVVATGTATLSESEEGVSAAISRVQQALQDAPTQGFFRVRLVE
                     "
     gene            1041..5675
                     /locus_tag="PLZ74_06905"
     CDS             1041..5675
                     /locus_tag="PLZ74_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOR98087.1"
                     /translation="MVRNGQRQGCGAPASDKVRRLAGPVWAHTVAAVFALALVAGRAH
                     AFRAEEGQTLLRSPVLRERRADRAGVRTAAQRTAQARAERAGVEVVWNDAEGVPISVR
                     GSDLGRTQALGGGRGLKPQGQKAYAADAIAALDDAAMLFGITDAAAEFTVRTLGSPDR
                     LGMSHVRLNQRVNGLRVVGTELIVHFDALDRVYEISGQYVPGIAAAPEPGIDARQALR
                     IAQADLAAMGYPAGTPEQEPERVIYAREVEPQPAYEIALRVRDQAAGLHALWRYWIDT
                     AQGVILVRYNDVKRVAPPSENGMEETITGTRLEREGGEVMDVQGWNEGNGLYYLYNPT
                     ALWCILNASTFDFFDYPDMGTYAYRESNDWGESDPVEISAACNVDAIQNYYQQVHGRN
                     SYDGKGGMADAYVHEGLFYANAYWDGTAMHIGDGDGTSCDSLAVLDIFAHEYTHAVTD
                     HTCDLVYQNESGALNESFSDIFGVCVEFANQPDGRDAYPDAIPGHADWLIGEDSWLEA
                     TALRDLRDPANPETVGAGNELPKLYMGTHWYTGMGDNGGVHYNCGVQNHFFYLLCEGG
                     SGTNDTSAYGYNINGIGVQAGEQIAYRALSKYCVPDDNYKTIMQRWVSAARDLDPRWV
                     RSTRAAWRAVLGMEPPPPMAVTSPSVLPDGRVGSWYSYLLKAKDGIAPYTWALLPEAP
                     KPDWLELAEDGTVSGLPPFEAVGTNEFTVTVVDVAGDVATNRFTFLIRNPFAIPYAES
                     FENNGQSPDSWTQSAVLNQQPWVFANGSPYGHPNSPYDGVYCARLAVLETRLTNSVTR
                     LETPMIDFGTDARAGRLTFRHIMANWEGGQDQLRVYYKNGYFEPWQLLAEFTDATENW
                     TLRSVDLPNLTRSYYLAFEGTANYGYGIHLDEVRIFDPTVPLDFATPEQLPDAYTERP
                     YTVALSVINGVGPFTFELVDGALPTGMVLSADGVISGTVDTVQSALFTVRVTDSQEPL
                     ATAERTFSLNVDLPVATLFFEDFENSGNLPAGWTQEYVERAVNWVCQKGGGNIIQYNQ
                     PSEAHSGGYNATLFQPTWDVYALNNNHITRLVTPPINLGEAPGSIRLVFWHCMIAFED
                     GQDELRVYYKNSAAGAWTLLAEYTENVPVWTQRTLQLPNPTATYYLAFEGNARFGHGV
                     CIDDVLIHDGASAPVITTDAWLPEATVGLPYTQTLTASGGVEPYTWAVVSNTLPVGLT
                     FSTNGVISGTPGSAGTTVFRVAVTGGDGYASTNLLTLRVKALPAGNFTETFDAGTGLP
                     EGWTQQITKGSFGWTFRKGSPENQPAAAHSPQTNACFYANGASYGNAIARLMTPVLNL
                     GTGTTNATLTFWEYRAADGASRDWLKLYYRNTLDGQWIWLTNFTETASSWTQRTVALP
                     ATSPTYALAFEGFSNSGYGIGLDDVEISGDFAGYASWQSTHFTPEELNDPAISGDEAD
                     PDHDGIPNGMEYAMGLDPWSPDAEGLIWGGIIDSYLTLTFRMGKQAFAAGTFYTVEAC
                     TNLLDQVWTVDQVTEYRRDDSNEWWQAFFRHDVPVTNAPQRFLRLRVTLP"
     gene            complement(5705..6802)
                     /locus_tag="PLZ74_06910"
     CDS             complement(5705..6802)
                     /locus_tag="PLZ74_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOR98088.1"
                     /translation="MTPPRHLPASEFRNRIRRLLARQRTLRLDAILLVTPVNRYYFTG
                     LDASNGVLLTEPGQTPSFHTDSRYLTMARRLAPWLPCRALWRPADEPAVLAGMGAGWR
                     RIGFEGRMDAARFLRLRAALPDAEWVDVTAETDRLRAVKSPAEQARLRAAVEANDRLF
                     AKLLRGIRPGMSEWTIRTLARREADRMGQGEAFDTIVCAGRNAAEPHHNPDDTLLRRS
                     QPLLIDLGLKLDHYCSDMTRCTVFGTPSRLFRELHGIVLKANRRAVRAIRPGRLCRDI
                     DALARDTIARAGYGAFFGHGLGHGLGLEVHETPSLSPACATVLEPGMVVTVEPGIYLP
                     GRLGVRIEDVVLVTGSGCEVLTRTPHQIGWS"
     gene            complement(6799..7737)
                     /locus_tag="PLZ74_06915"
     CDS             complement(6799..7737)
                     /locus_tag="PLZ74_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOR98089.1"
                     /translation="METWFDANLNTLTEALSPCKNCPVANTERDVNLPGTGAVIAVLD
                     SLLAVLYPGCHGQSPVPHENGPAHLKDTLREIALRLHDQTHRAFEYACRNAKCEDCRD
                     CEAHARQAVTALLSGLPAIQNMLQEDIQAAYEGDPAAMSLMEIVMSYPGLYAITVQRI
                     AHALYEQGVPLIPRVMSEHAHSRTGIDIHPGAVIGPGFFIDHGTGVVIGETCRIGRRV
                     KIYQGVTLGALSFAKDEVTGMLVKGIKRHPDVEDNVVIYAGATILGGDTVIGHDSVIG
                     GNVWLIHSVPPGSKVYNQTPKPMIQTGSSIQNDYTV"
     gene            complement(7739..9583)
                     /locus_tag="PLZ74_06920"
     CDS             complement(7739..9583)
                     /locus_tag="PLZ74_06920"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOR98090.1"
                     /translation="MDKNTHTTTEIGVVTTQSVRLELPADGFRLEKGGVLREITVAYE
                     RCGAITAANDNVIFICHALTGDAHVAGLRPGESRPSGWWEGMIGPGKGIDTRRYQVVC
                     ANILGGCKGTTGPSSINPDTGKPYGSAFPKITVGDIVEVHRMALRQIGITRLAGVMGG
                     SFGGMQVLEWLIRYPHEIERGIVIASAASLNSQALAFDIVGRHAIIGDPNWQTGDYYD
                     SENLPDKGLSSARKLAHITYLSQQLMDSKFGREKRPEWLTADSDFRNRIEREFGTFFQ
                     VESYLQHQGEKFTQRFDANSYLHITLAMDEYDIEERYGSLDKAFAAVASRVLIVSLSG
                     DWLFMPEQSEELVTALVRQKKRVSYCHLKVAAGHDAFLTHTEELKRVIQAFVPPATES
                     GEAAPIRQWQLRHYQQVINLIPPGSRVLDLGCGNGSLLNILRQLHGCSGNGVEIDLQQ
                     VISAISIGNDVLLEDIDDGLAMIPDGSFDVVVLSETLQTIKRPRELLQQILRVAREAV
                     ISFPNFACFEVRRELLLKGRMPKGKQLPFEWYNTPNIHLFTLKDFRDLCRKDNIQILD
                     LVCRGRGFVERLLLSLGWNNLGASQVIVRIAQGSEDAAPPPPNPTGKG"
BASE COUNT         1739 a         3006 c         3271 g         1813 t
ORIGIN      
        1 tccgggccgt ggtgaagtat ttcggtccgg gtaagaacct gaccgacaag gtcaaggtgc
       61 aggcgttcca caacgccgcg ttcagcggca tgccggtggc ggagtacacc ctcgcgggcg
      121 gaaacctcgc ggccgtgacc aatacggcga atgtggtcac caacgccata atctgcggtc
      181 tggcgcccag tgccgaggcc ggcgattact acctgcgcgc ctacatcgac agcaacggaa
      241 acaacgtgcg ggatgtctgg gaatcgtggg ggtatgccaa cttctacggc gtcagcgaca
      301 aaccgttcga tccgcggccg gtcacggtcc agttcgtgcc gctggcggaa agcccgacgg
      361 taacgatcct gatcgaggat gccgacacgg atcaggactg gttcccggat atctacgagt
      421 acgagacgaa tccgggcgtc ggcttcttgt cgaagtccgg tccgtccacg gggagtgacg
      481 gcgaccctga cttcgagatc aatccgaatc tgggcgacac gaccgggatc aacacgctct
      541 tctcggccct gatgttaggc agcacggacg ccgacggtga cgaggtgggt gatctggcgg
      601 aactgatcct cggttcggac ggccagaagg tctccacttc cggtgacggg gtttccgacg
      661 gtctcaagat cggtctgggg ctctcgccgg ccgacacgct ggcactggag atgaccggac
      721 tggaactcac cggcagcgag gcgaccctca cctggaaact ggtggtggat caggctgaag
      781 gggtgaaccg caacctgatc aagagccttg ccggcgcgcc cggaccggcg acctacgaag
      841 tgctcttcaa gccttcgctg gatgcggccg actggcaggt ggtggccacg ggcacggcca
      901 cgctttcgga aagtgaagag ggcgtgagcg cagcgatcag ccgtgtgcag caggcattgc
      961 aggatgcgcc gacacagggc ttcttccgag tccgtctggt ggagtaacgg gaacggcgga
     1021 gtatacgcgg agtttcgcac gtggtaagaa acggacagcg tcagggatgt ggggcaccgg
     1081 cctcagacaa ggtccgccgg ttggcgggcc ctgtctgggc gcatacggtg gcggcggtgt
     1141 tcgccctggc cctcgtcgcg ggaagggcgc atgcgttccg ggccgaggag gggcagacct
     1201 tgttgcggtc gcccgtgttg cgtgagcggc gcgctgaccg tgcgggggtg cgcacggcgg
     1261 cgcaacggac ggcgcaggcc cgggccgaac gcgcgggtgt cgaggtggtg tggaacgacg
     1321 ccgaaggcgt gccgatctcg gtccgcggct cggatctcgg gcgaacgcag gctttgggcg
     1381 gcggcagggg gctcaagccc caggggcaga aggcgtatgc tgcggatgcg atcgcggcgc
     1441 tggatgacgc cgcgatgctt ttcggcatca cggatgcggc ggcggagttc acggtccgca
     1501 cccttggttc gccggaccgg ctcgggatgt cgcatgtgcg gctcaaccag cgggtcaacg
     1561 gtctgcgggt ggtcggcacg gaactgatcg tgcactttga cgcgctggac cgcgtctatg
     1621 agatcagcgg tcagtatgtg cccgggatcg cggccgcgcc cgaacccgga atcgacgcgc
     1681 ggcaggccct gcggatcgcg caggccgatc tggcggccat gggctatcct gcgggaacgc
     1741 cggagcagga gcccgagcgg gtgatctacg cccgcgaagt cgagccgcaa ccggcctatg
     1801 agatcgcgtt gcgcgtgcgg gatcaggccg ccggacttca cgccctctgg cgctattgga
     1861 ttgatacggc gcagggtgtg atccttgtcc gctacaacga cgtcaaacgg gtcgcgcctc
     1921 cgagtgagaa cgggatggag gagacgatca ccggcacccg cctcgagcgc gaggggggcg
     1981 aggtgatgga tgtgcagggg tggaatgagg gaaacggcct gtactacctc tacaacccga
     2041 ccgcgctctg gtgcatcttg aacgcttcga cgttcgactt cttcgactat cccgatatgg
     2101 ggacctacgc ttaccgcgaa tcgaacgact ggggggaatc ggatccggtc gagatttccg
     2161 ccgcctgtaa tgtggatgcg atccagaatt actaccagca ggttcacgga cgcaacagtt
     2221 acgacggaaa aggcggcatg gccgatgcgt atgtgcatga ggggttgttt tacgccaatg
     2281 cctattggga cggaaccgcg atgcatatcg gcgacggcga cggcacgagt tgtgatagtc
     2341 tcgcggtgct cgacattttc gcccatgaat acacccatgc cgtgacggac cacacctgcg
     2401 atctcgtgta tcaaaacgag tccggcgcgc tgaacgagtc cttttccgat attttcggtg
     2461 tttgcgtcga attcgcgaac cagccggacg gacgcgatgc gtatcccgac gcgattccgg
     2521 gtcatgccga ctggctgata ggtgaggact cctggctgga agccaccgcg ctgcgcgacc
     2581 tgcgcgaccc ggccaatccc gaaacggtcg gcgcgggcaa tgaactgccc aaactctata
     2641 tgggaaccca ttggtacacg ggtatgggcg acaacggcgg ggtgcactac aactgcggcg
     2701 tgcagaacca cttcttctat ctgctctgcg agggcggatc cggaacgaac gacacttccg
     2761 cctatggcta taacatcaac gggatcggcg tgcaggccgg cgaacagatc gcctaccgcg
     2821 cgctgtccaa gtattgcgtg ccggacgaca actacaaaac cattatgcag cggtgggtct
     2881 ctgcggcgcg ggatcttgac ccccgttggg tgcgcagcac gcgggcggcc tggagggcgg
     2941 ttctgggcat ggaaccaccg cctccgatgg cagttacctc gccgtccgtg ctgccggatg
     3001 gccgggtcgg ttcttggtac agctatctgc tcaaggccaa agacgggatc gcgccgtata
     3061 cctgggcact gcttccggaa gcgcctaagc ccgactggct ggaactcgcg gaggacggaa
     3121 cggtctcggg cctgccgccg ttcgaagcgg tcggcacaaa cgagtttacg gtgacggtgg
     3181 tggatgtcgc gggtgatgtc gcgaccaacc gtttcacgtt cctgatccgc aaccctttcg
     3241 ccattccgta cgcggagtcc tttgaaaaca acggccagag ccccgacagt tggacgcaga
     3301 gcgcggttct gaatcagcag ccctgggttt tcgcgaacgg cagcccctac ggccatccca
     3361 acagcccgta cgacggcgtc tactgcgccc ggctggcggt gctggagacg cggttgacca
     3421 actccgtcac gcgcctggag acgccgatga tcgatttcgg caccgacgcc cgcgcgggcc
     3481 ggctgacttt ccgtcacatt atggccaact gggagggcgg tcaggaccag ttgcgcgtgt
     3541 attacaaaaa cgggtatttc gagccgtggc agcttctggc cgagttcacc gacgccaccg
     3601 agaactggac cctgcgcagt gtggatctgc cgaacctgac ccgctcctac tatctggcct
     3661 tcgagggcac ggccaattac ggatacggca ttcatctcga cgaggtccgg atcttcgatc
     3721 cgaccgtgcc gctggatttc gccacgccgg aacagttgcc ggacgcctac acggaaagac
     3781 cctacacggt tgcgttgtcc gtgatcaacg gcgtgggccc gttcaccttt gagctggtcg
     3841 acggcgcact gccgaccggg atggtcctga gtgccgacgg tgtgatcagc ggaacggttg
     3901 acacggttca gagcgccctc ttcaccgtgc gggtgaccga ttcgcaggaa cctctggcca
     3961 cggctgaacg gactttcagt ctcaatgtgg acctgccggt cgcgacgctt ttcttcgagg
     4021 atttcgagaa cagcggcaat ctgccggcgg gctggacaca ggagtatgtg gagcgcgccg
     4081 tgaactgggt gtgccagaaa ggcggcggca acatcatcca gtacaaccag ccttcggagg
     4141 cccacagcgg cggctacaac gccacgttgt tccagcccac atgggatgtg tacgccctga
     4201 acaacaacca catcacccgg ctggtcacgc cgccgatcaa tctgggcgag gcgccgggca
     4261 gcatccgtct ggtgttctgg cactgcatga tcgcgttcga ggacggccag gatgagctgc
     4321 gggtctacta caagaactcg gccgccgggg cgtggaccct gctggcggag tataccgaga
     4381 atgtgccggt ctggacccag cggacactcc agcttcccaa ccccaccgcg acgtattatc
     4441 tggctttcga gggcaacgcg cgtttcggcc acggcgtgtg catcgacgat gtcctgattc
     4501 acgacggcgc gagcgcgccg gtcatcacga ccgacgcatg gctccccgaa gccacggtgg
     4561 ggctgccgta cacgcagacc ctgacggctt ccggcggggt ggagccctac acttgggccg
     4621 tggtctcgaa caccttgccc gtaggcctca ccttcagcac caacggggtg atcagcggta
     4681 cgcccggaag cgcgggcaca accgtgttcc gtgtggcggt gaccggcggg gacggctatg
     4741 cctcgaccaa tcttctcacc ctgcgggtga aagccctgcc cgcagggaac ttcaccgaga
     4801 cctttgatgc cggaaccggt cttcctgagg gctggaccca gcaaatcacg aaaggttcgt
     4861 tcggctggac cttccgcaag ggcagtccgg aaaaccagcc cgcggcagcc cactcgcccc
     4921 aaaccaacgc ctgtttttac gccaacggcg catcgtacgg gaacgccatc gcccgactca
     4981 tgaccccggt gctcaatttg ggcaccggca caaccaacgc gacgctcacc ttctgggagt
     5041 accgggctgc ggatggggca tcccgcgact ggctgaagct gtactaccgg aacacgcttg
     5101 acggccagtg gatttggttg acgaatttca cggagaccgc ttcctcgtgg acccagcgca
     5161 cggtggcact gcccgcgacc agcccgacct acgcgctggc ctttgaaggg ttttccaaca
     5221 gcgggtatgg catcggtctg gacgatgttg aaatcagcgg cgatttcgcc ggatacgcct
     5281 cttggcagag tacgcatttc actccggaag agctgaacga tcccgcgatt tccggcgatg
     5341 aggccgatcc ggaccatgac gggattccga acggcatgga gtacgccatg ggtttggatc
     5401 cgtggtcgcc cgatgcggag ggtctgatct ggggcgggat catcgactcc tatctcacgc
     5461 tcaccttccg catgggcaag caggcgtttg ccgcaggcac gttctatacg gttgaagcct
     5521 gcaccaattt gttggatcag gtctggaccg ttgaccaggt cacggagtat cggcgcgatg
     5581 acagcaatga gtggtggcag gccttcttcc gccacgatgt gccggtcacc aacgcgcccc
     5641 agcgcttcct gcgtctacgg gttacactgc cttgaccttt ttgcgggccc gttcgggctt
     5701 ggtctcagct ccagccgatt tgatgcggcg tgcgggtgag cacctcacag ccgctaccgg
     5761 tcacgagcac gacatcctcg atgcgtaccc ccagccgtcc cggcaggtag atgccgggtt
     5821 ccacggtcac caccatgccc ggttcgagga ctgtcgcgca tgcgggtgag aggctggggg
     5881 tttcgtggac ctccagtccc agaccgtgac ccaggccgtg accgaaaaaa gcgccgtaac
     5941 ccgcccgcgc gatcgtatcc cgtgcgagcg cgtcgatgtc gcggcagagg cgtcccggcc
     6001 ggatggcccg cacggcccgg cggttggcct tcagcacgat gccatgaagt tcacggaaga
     6061 gccgggaggg tgtgccgaag acggtacagc gggtcatatc cgagcagtag tggtccagtt
     6121 tgagcccgag atcgatcagc aacggttggc tgcggcgcag aagcgtgtca tccgggttgt
     6181 ggtggggttc cgcggcgttg cgacccgcgc agacgatcgt gtcgaacgcc tcgccctgac
     6241 ccatccggtc agcctcccgg cgggccagcg tgcggatcgt ccactcgctc atgccgggcc
     6301 ggatcccgcg cagcagtttc gcgaacaggc ggtcgttcgc ctcgactgcc gcgcgcagcc
     6361 gggcctgttc ggcaggggat ttgacggcgc ggaggcgatc cgtctccgcc gtcacgtcaa
     6421 cccactccgc atccggcagc gcggcacgga ggcgcaggaa gcgcgcggcg tccatgcgcc
     6481 cctcgaagcc gatgcggcgc catccggcac ccatgccggc cagcacggcg ggttcgtcgg
     6541 cgggacgcca cagtgcgcgg cagggcagcc agggcgccag acgtcgcgcc atggtgaggt
     6601 atcgggaatc cgtgtgaaag gagggggtct gacccggctc ggtcaacaac acgccgttcg
     6661 aggcatcgag gccggtgaag taatagcggt tgaccggagt gaccagcagg atcgcatcca
     6721 gccgcagtgt gcgctggcgg gccagcagac gtcggatgcg gttacggaac tcggaggctg
     6781 ggaggtggcg cggcggcgtc ataccgtgta atcgttctgg atcgaagacc cggtttggat
     6841 catcggcttg ggtgtctggt tgtagacctt ggaacccggc ggcaccgagt ggatcagcca
     6901 gacgttgccg ccgatcacgg agtcatggcc gatcaccgtg tcgccgccga gaatggtggc
     6961 ccccgcatag atcaccacgt tgtcctcgac atccgggtgg cgtttgatgc ctttgaccag
     7021 catgcccgtg acctcgtctt tggcgaaact gagcgcgccg agcgtgaccc cctggtagat
     7081 tttgacgcgg cgtccgatac ggcaggtctc gccgatcacc acgccggtgc cgtggtcgat
     7141 aaaaaagccg ggtccgatca cggcgccggg gtggatatcg atgccggtgc gcgagtgggc
     7201 gtgttcgctc atcacgcgcg ggatcagcgg gacgccctgc tcgtagagcg cgtgggcgat
     7261 gcgctggacc gtgatcgcgt acagccccgg ataactcatc acgatctcca tgagggacat
     7321 cgcggccggg tcgccctcgt aggcggcctg gatatcctcc tgaagcatat tctggatcgc
     7381 gggcaggccg cttagcagcg ctgtgaccgc ctgacgggcg tgtgcttcac aatcgcggca
     7441 atcctcgcat ttggcgtttc ggcaggcata ctcgaaggcg cggtgcgtct gatcgtgcag
     7501 ccgcagggcg atttcgcgca gggtgtcctt caggtgcgcg ggtccgttct cgtgcggcac
     7561 gggggattgg ccgtggcagc cggggtagag cacggccaga agcgagtcca gcacggcgat
     7621 caccgcaccg gtgcccggca ggttgacatc gcgttccgtg ttggcgaccg ggcaattctt
     7681 gcagggggag agcgcttcgg tcagggtgtt gagattcgcg tcaaaccagg tttccatatc
     7741 agccctttcc ggttgggttg gggggcggcg gggcggcgtc ttcgctgcct tgggcgatac
     7801 gtacgatcac ctgcgaggca ccgaggttgt tccagcccag agagagcagg agccgctcca
     7861 cgaatccgcg gccccggcag accagatcga ggatctggat gttgtccttt cggcagaggt
     7921 cgcggaagtc tttgagggtg aagaggtgga tgttgggcgt gttgtaccat tcgaagggca
     7981 actgcttacc tttgggcatg cggcctttga gcagcagctc gcgccgcacc tcgaagcagg
     8041 cgaagttcgg gaaggagatc accgcctcgc gcgccacgcg cagaatctgc tggagcagct
     8101 cacgcggccg cttgatggtc tgcaacgttt cgctgagcac caccacgtcg aagctgccgt
     8161 ccggaatcat ggcgagcccg tcgtcgatat cctcaagcag cacgtcgttc cctatgctga
     8221 tggcggagat gacctgctgg aggtcgatct cgacaccgtt gccgctgcag ccgtggagtt
     8281 gccgcaggat gttcagcagc gacccgttgc cgcaacccag atccagcacg cggctgcccg
     8341 gcgggatcag attgatcacc tgctggtaat ggcgcaactg ccactgccgg atcggcgcgg
     8401 cttcgccgga ctccgtcgcg ggcggcacga aggcctggat cacgcgcttc aactcctcgg
     8461 tgtgcgtcag aaacgcgtca tgtccggcag ccactttgag gtggcagtag gatacccgct
     8521 tcttctggcg cacgagcgcg gtgaccagtt cctcggattg ttcgggcatg aagagccagt
     8581 cgccgctgag cgagacgatc agcacgcgcg aggccacggc ggcgaacgcc ttgtccagcg
     8641 aaccgtagcg ctcctcgatg tcgtactcgt ccatggccag cgtgatgtgc aggtaggagt
     8701 tggcgtcgaa gcgctgggtg aacttctctc cctgatgctg cagataactc tcgacttgaa
     8761 agaacgtgcc gaattcgcgc tcgatgcggt tccggaagtc ggaatcggcc gtgagccact
     8821 cggggcgctt ctcgcggccg aacttggagt ccatgagctg ttgcgacaga taggtgatgt
     8881 gcgccagttt gcgcgcgctg gaaagcccct tgtcgggcag attctcgctg tcgtagtaat
     8941 cacccgtctg ccagttcggg tcgccgatga tcgcgtgccg accaacgatg tcaaaggcca
     9001 gcgcctggga gttcaggctc gccgcggaag cgatcacgat tccccgctcg atctcgtgcg
     9061 ggtaacggat cagccactcc agcacctgca tgccgccgaa actgccgccc atcacacccg
     9121 ccagccgcgt gatgccgatt tggcgcaggg ccatgcggtg gacctccacg atgtcgccca
     9181 ccgtgatctt cggaaaggcg gagccgtagg gttttccggt gtcggggttg atcgaggagg
     9241 gcccggtggt gcctttgcac ccgccgagga tgttcgcgca cacgacctga tagcgccggg
     9301 tgtcgatccc cttgcccggg ccgatcatgc cctcccacca gccgctcggc ctgctctcgc
     9361 ccggacgcaa gcccgccaca tgggcgtcgc cggtgagcgc gtggcagatg aagatcacat
     9421 tgtcgttggc cgcggtgatc gcgccgcagc gctcataggc caccgtgatc tcccgcagca
     9481 cgccgccttt ttcgaggcgg aatccgtctg cgggaagctc cagccggacg ctctgcgtcg
     9541 ttaccacacc gatttctgtt gtcgtatgcg tgtttttgtc catgttcctt tcagaaaacg
     9601 gggaaagtct aacatggcgc actctgtccg gcaattctcc ttttgccgga ctgcgggcgt
     9661 tcccgcccgt tggtagggac ggttctccga accgtccgcg gacgccttaa aaagagcgtt
     9721 gaaagcaacg ctctttggtt tcatggtttc ggggtttctg gtttcagggt ttctgggttt
     9781 ctggtttcat ggtttcatcc aaatcggggt cggggtcggg atcgctatc
//
LOCUS       DAOSRB010000105         9798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6687_length_9798_cov_7.729652, whole genome shotgun sequence.
ACCESSION   DAOSRB010000105 DAOSRB010000000
VERSION     DAOSRB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6687_length_9798_cov_7.729652"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLZ74_06925"
     CDS             <1..261
                     /locus_tag="PLZ74_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98091.1"
                     /translation="RHTVTDTASVTKAVNTRKGKDMNETKTKIVKETRTVTRFRGLGK
                     AARELGVSRGHLSYVLHGQRKPGKDLERKLRRMGITPGGRAV"
     gene            258..581
                     /locus_tag="PLZ74_06930"
     CDS             258..581
                     /locus_tag="PLZ74_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98092.1"
                     /translation="MSEVVIRVVVEQRPAPAPAGAAVWAPKGGRRAVTPAPKYGIGKK
                     LYYKPASRKGWHSAKFVVADLRIHAVRGVSYHDWDGSGHREDDVEPNAEAAFKRWPAK
                     DLPEA"
     gene            644..1117
                     /locus_tag="PLZ74_06935"
     CDS             644..1117
                     /locus_tag="PLZ74_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98093.1"
                     /translation="MNNRQQVYLRWQGRRGGGAWQTAGSGPWDVIAADDGAVLLDHAD
                     WAELERERDRIERGLGVTGAVLQASRPEGGWCETWETVAYARRGVGTGLEIGHTTYPI
                     EAIRKGASYYQFRVVVPCGARAAAALSRQYGVYEAVGRGWCERIGYEAVDFRLPA"
     gene            1147..1482
                     /locus_tag="PLZ74_06940"
     CDS             1147..1482
                     /locus_tag="PLZ74_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98094.1"
                     /translation="MSAWKPIKDRGVNGIQRIWSRYGSPTAVADIRCVPSADGFEYYV
                     CCGASAGGDSGTVIARARTDDMESAKAVASALAKFARSKHARLARRKAVRAARANMAL
                     PGDDLAARS"
     gene            1496..1717
                     /locus_tag="PLZ74_06945"
     CDS             1496..1717
                     /locus_tag="PLZ74_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98095.1"
                     /translation="MRNTIKQMAGDLRAIRRALEAEVAMRAVRRGLEETDERAEFAQI
                     LAGLKRRGVITQDDIERLERDGTKEVRHA"
     gene            1710..2060
                     /locus_tag="PLZ74_06950"
     CDS             1710..2060
                     /locus_tag="PLZ74_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOR98096.1"
                     /translation="MRNMSFSLTTAQVRAQTKDVTRRIGWADLKPGERLMAIEKGQGL
                     KKGEKVRRITVIECVSNRKEMLCAITPHEVLREGFPDMSPADFVEMFCRANKCGPRDY
                     VNRIEFRYVEVRHG"
     gene            2053..2376
                     /locus_tag="PLZ74_06955"
     CDS             2053..2376
                     /locus_tag="PLZ74_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98097.1"
                     /translation="MGEAKRRGAFGERRAAARIRDGYGRTINAERPPSSQTEERLACF
                     LEWALVIGIAVLTAVAALAVGRQAARAWRERGRRPPEAALYEMNWRRGIYGDELCGFT
                     REGGL"
     gene            2373..2729
                     /locus_tag="PLZ74_06960"
     CDS             2373..2729
                     /locus_tag="PLZ74_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98098.1"
                     /translation="MTDLPGQLTLFPHLDRPSAGAAATDPRADAVRALNEGLPDKSTV
                     SVPEAAKIMHCCRRTVENWIADGTLLATYANRSDEARRKHARIIVRAGREYDPRRTRF
                     LTLAELRIRRSNVGGN"
     gene            2767..3489
                     /locus_tag="PLZ74_06965"
     CDS             2767..3489
                     /locus_tag="PLZ74_06965"
                     /inference="COORDINATES: protein motif:HMM:NF022807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HOR98099.1"
                     /translation="MSRMTTINIECSEQAAEALRPLFAEMARAYERAAAGQAKQKESV
                     PAGHVRNAKGWLMPVASVKQQDLLEDALVDRCHLMMAAVAEGCRMMRLGTHSECDALI
                     GMIVEDAGGDGATQVGKATLQNIGATRRIMIDRRDTLAFGAEVTAARDKVMACVRKWS
                     DGANPHLVALANAAFQANGNGDLSVSKVASLWRMECDDPEWQDAMTALKDSLRVAGQT
                     TYIRMHERQDPDGKWELIEAKV"
     gene            3522..4256
                     /locus_tag="PLZ74_06970"
     CDS             3522..4256
                     /locus_tag="PLZ74_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98100.1"
                     /translation="MKKTNEDAVVVAADARPVDVTAMKTIIGEDLNRAEVGVFCMLRA
                     GVGLLVYKELSEHGEWEDRLLELGKGKSPRTLQIYMKQARTLCEQYGITPAQAWDEAR
                     HIDASQVTELLLAAPGEPRQIGAGEQPAEDPPAKKGRKAKKSKPASGPDMPTQTFAQM
                     LLDFMEQRKRAKAKDIEPKKPLTKKEKIETAIAEAFRVVNLTADWVADGTWALLPDEE
                     LESTMAGLRAAADKIRDEFRNRRAKI"
     gene            4270..6324
                     /locus_tag="PLZ74_06975"
     CDS             4270..6324
                     /locus_tag="PLZ74_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98101.1"
                     /translation="MATTVLSVRMQSPEFAALPSEERASVLVWEDVMRQIDAAGNKLA
                     MCREWAQRNGHRRGWSADRIRAKYYGWVASGRAWTFLVDWARVPRTPAPERRGLASAF
                     KRYCEENQRASKPAWRAMLRDLRRGVHIPGVGTWRDVWAGQFPGETIPERCPSDWIPR
                     GWRYSNMIRKYKSTKFELVAMRKGAAAARQFAPAVYSTRVGLLPGQIYQFDDMWHDVK
                     IAVPGVNRKLLRPLEFACVDVASTYKAGWGIKPQIQREDGSREGLAAGQFKALVAHIL
                     CNVGWHPDGCQMVIEHGTASLNDADQALVTRLTDGAVTFRTSGILGGQVLRGMFRGQG
                     RGNFKVKALIEGSHRQPHYEAASLPAQTGGLSRVDEPEQMYGLDRYADRVLAAWDRVP
                     EEQRNLLWFGGALTLSAYTRIIADIYDAIYTRTDHDIEGWEQNEWMLLQWSVDGHSHW
                     YTVDDIPKLDPEMQQIGRIALNKPGLTRLRRMSPHEVWERHKDGLKRLPHWAVVDFLG
                     DECFRRVRVGHDGLIAFQDRDLTGSAVKLRFAGAVMQPDGTGCRLAPGAEYGLYVLPH
                     DLTKAVIVEAGTRAVLGIAPAWSAVDPLNAAQVAVMQEAQAKMIALQSQGVRERHAER
                     GDELVVRREENDLLLAGLTEPRRQKTKKQAKQKKQAAGQAMRALAGMRSDVNESEVED
                     EW"
     gene            6314..7261
                     /locus_tag="PLZ74_06980"
     CDS             6314..7261
                     /locus_tag="PLZ74_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR98102.1"
                     /translation="MSGNMARANYDTDVREALQAFQDKNGLTLKQLGARLGKNEGFVS
                     KYLNGVGEGNIAAFEKRVKDMLERESRKRTWESVCFHTDAVEACYTVFDLVREASDIG
                     LITSEAGIGKSMAVHQYMIEHKTVIGFTAIEGSGSQYDVQRGIASCLDMRKFNPRSCR
                     LSDYLREKLAGSERLVIIDNAQRIFLSGLRWIMDFHDATGVSFCLVGNPDVMERLEGR
                     DQLVSRIGIKKDITSALGNEKWIDDAADRMVAAMWPKAASEIAILAHEAARKSGHLRT
                     LNKQLRIAIRLCETDMYRNRYAKSFVVARDLIGAGDDED"
     gene            7587..8075
                     /locus_tag="PLZ74_06985"
     CDS             7587..8075
                     /locus_tag="PLZ74_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOR98103.1"
                     /translation="MERPETLTDLERAARYYYLQRLSFGGKVKGRTFGYSATSAPKLD
                     IGSIQSDLLDVHMRLARVTIEHLDGMDCIRRYDRKESLFFIDPPYLDVTGYEVPFPKD
                     RYNELAELLSSVKGRFILTLNNHPQIRETFKRFKISEVQTSYSTGLEAKQRVTELLIT
                     NR"
     gene            8247..8699
                     /locus_tag="PLZ74_06990"
     CDS             8247..8699
                     /locus_tag="PLZ74_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98104.1"
                     /translation="MLRFAVLAYDDSAIGYWSACVERRLRWVLKGLATDLQYLQTTGV
                     GEEYIAGIYAQAGMLPADFEDAPVQHLWCCLQILDTRIRSLCKRDGIALHLLPTAGPP
                     WQFRGVRAARYAAFVALSADRARRDAGLPTDATAEPSGAPVRDVAATA"
     gene            8747..9382
                     /locus_tag="PLZ74_06995"
     CDS             8747..9382
                     /locus_tag="PLZ74_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98105.1"
                     /translation="MSHQIEGFFLRKSLIFRVLPRFSALCQSGSWCTSTNALFLGLCT
                     SERSRRLLLESKDHPDPAVRDSVRLALARRGDSDYARQFIQQYQASPISRKLTVALAK
                     DREATKTVRRLEYIGTPDALTALFDGAASHALAEEPFSGSNRVIVHSTTLQNFRDFMT
                     ALGVSIPDTVSTGRELANWWKENRERLVALMRQKESTLPRIRGTRFGIGYL"
     gene            9429..>9798
                     /locus_tag="PLZ74_07000"
     CDS             9429..>9798
                     /locus_tag="PLZ74_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98106.1"
                     /translation="MKLRTHVFGGMVLLACSLHLFAEVENDSIQYIDAKSVPVIRLND
                     ERQKPFAELWDKYKASSSAAYRRVIVSLMTERENMEVTDSIDCEICSSFAENQTADAA
                     LLLGMCNSGRSRDLLRQHTEA"
BASE COUNT         2013 a         2728 c         3446 g         1611 t
ORIGIN      
        1 cgtcacaccg taacagacac cgcaagtgta acaaaagcgg ttaacacgag aaagggcaaa
       61 gacatgaacg aaacgaaaac gaaaatagtc aaggagacgc ggacggtgac gcggttccgc
      121 gggctgggaa aggcggcgcg tgagctgggc gtctcgcgcg ggcacctgag ctatgtgctg
      181 cacggccagc gcaagccggg caaggacctg gaaaggaagc tgaggcggat gggcatcacg
      241 ccgggagggc gggcggtatg agcgaggtgg taatccgcgt ggtggtggag caacgcccgg
      301 cacccgcccc ggcgggggcg gcggtctggg cgccgaaggg cgggaggcgg gcggtgacgc
      361 ccgcgccgaa gtacgggatc ggcaagaagc tgtactacaa gccggccagc aggaagggct
      421 ggcacagcgc gaagttcgtg gtggcggatc tccgcatcca cgcggtgcgc ggcgtgagct
      481 accacgactg ggacggcagc gggcacaggg aggacgacgt ggagccgaac gcggaggcgg
      541 cgttcaagcg gtggccggcg aaggatctgc cggaggcgta gaggttttcc cggcgcggcg
      601 gtcgcgccgg ggcgtagcaa agcaacgagg aaggagacag gcgatgaaca acaggcaaca
      661 ggtttatctg cgctggcagg gccggcgcgg cggcggggcg tggcagacgg cgggcagcgg
      721 cccgtgggac gtgatcgcgg cggatgacgg cgcggtgctg ctggaccacg cggactgggc
      781 ggagctggag cgcgagcgcg accgcatcga aaggggcctg ggcgtgaccg gcgcggtcct
      841 gcaggcgagc cggccggagg gcggctggtg cgagacgtgg gagaccgtgg cgtacgccag
      901 gcgcggcgtc gggacggggc tggagatcgg gcacacgacg taccccatcg aggcgatcag
      961 gaagggcgcg tcctactacc agttcagggt ggtggtgccc tgcggcgcga gggcggcggc
     1021 ggcgctgtcg cggcagtacg gcgtgtatga ggccgtcggc cgggggtggt gcgagcgcat
     1081 cggctacgag gcggtggact tcaggcttcc ggcgtgacgg caagcgacca agaaaggagg
     1141 cggcaagtga gcgcgtggaa accgataaag gacaggggcg tgaacgggat ccagcggata
     1201 tggagccggt acggctcgcc gacggccgtc gcggacatcc gctgcgttcc gtccgcggac
     1261 gggttcgagt attacgtgtg ctgcggcgcg agcgcgggcg gcgactccgg cacggtgatc
     1321 gcgcgagcgc ggaccgacga catggagtcc gcgaaggcgg tcgcgtcggc gctcgcgaag
     1381 ttcgcgcggt cgaagcacgc gcggctggcg cggcgcaagg ccgtccgggc ggcgcgggcg
     1441 aacatggcgc tgcccggcga tgatctggcg gcgcggagct aggaggagac aggaaatgcg
     1501 gaacacgatc aaacagatgg cgggcgacct gcgggcgatc cggcgggcgc tggaggcgga
     1561 ggtggccatg cgggcggtcc ggcgggggct ggaggagacg gatgagcgcg cggagttcgc
     1621 gcagatcctc gcggggctca agaggcgcgg ggtgatcacg caggatgaca tcgagcgtct
     1681 ggagcgggac gggaccaagg aggtgcggca tgcgtaacat gtcattctcg ctgacgaccg
     1741 cacaggtacg cgcgcagacg aaggatgtca cgcggcgtat cgggtgggct gatctaaagc
     1801 cgggtgaaag gctcatggcg atcgagaaag gccagggcct caagaagggt gagaaggtgc
     1861 ggcggattac cgtgatcgaa tgcgtcagca acaggaagga aatgctctgc gcgatcacgc
     1921 cgcatgaggt gctgcgcgaa ggctttcccg acatgtctcc ggctgatttt gtggagatgt
     1981 tctgcagggc caataaatgc ggaccgcgtg attacgtaaa ccgtatagag ttccgttatg
     2041 tggaggtgcg gcatgggtga ggcgaagcgc agaggggcat tcggtgagcg ccgggcggcg
     2101 gcgcggatca gagacggata tggcagaacg atcaacgccg aacgtccgcc ttccagtcag
     2161 acagaggaaa ggctggcctg ttttctggag tgggcgctgg tgatcgggat cgctgtgctg
     2221 acggcggtgg cggcattggc cgtcgggcgg caggcggcgc gggcgtggcg ggagcggggg
     2281 cggcgcccgc cggaggccgc gctttatgaa atgaactggc ggcggggcat ctacggcgac
     2341 gagctttgcg gcttcacccg ggagggcggc ttgtgacgga ccttcccggc cagctcacgc
     2401 ttttcccgca ccttgaccgg ccgtccgccg gcgcggcggc caccgacccc cgggcggacg
     2461 ccgtgcgggc gctgaacgaa ggtctgccgg acaagagcac ggtttccgtg ccggaggccg
     2521 ccaagatcat gcactgctgc cggcgcacgg tggagaactg gatcgcggac ggcacgctgc
     2581 tggcgaccta cgccaaccgc agtgacgagg cccggcgcaa gcacgcccgc atcatcgtgc
     2641 gggcaggtcg cgagtacgac ccgcggcgga cacgatttct gacgctcgcg gagctgcgca
     2701 tacggcgcag caacgtgggc ggcaactaaa cgcgcctgca aggcgcttgc aaggaggacc
     2761 agacaaatga gcaggatgac gacgatcaac atcgaatgca gcgagcaggc ggcggaggcg
     2821 ctgcggccgc tgttcgcgga aatggcgcgc gcctacgaga gggctgccgc cggccaggcc
     2881 aaacagaagg agagtgtccc ggcggggcat gtccgcaacg cgaaagggtg gctgatgccc
     2941 gtcgcgagcg tgaagcagca ggacctgctc gaggacgcgc tcgtcgaccg ctgccacctg
     3001 atgatggcgg cggtcgccga ggggtgccgg atgatgcgcc tcggcacgca cagcgagtgc
     3061 gacgcgctga tcggcatgat cgtggaggac gcgggcggcg acggcgcgac ccaggtcggc
     3121 aaggccacgc tccagaacat cggcgcgacg cgccggatca tgatcgaccg gcgcgacacg
     3181 ctggcgttcg gcgcggaggt cacggcggcg cgcgacaagg tcatggcctg cgtgcgcaag
     3241 tggagcgacg gcgcgaaccc gcacctggtg gcgctggcca acgcggcctt ccaggcgaac
     3301 ggcaacggcg acctgagcgt gtcgaaggtg gcgtcgctct ggcgcatgga gtgcgacgac
     3361 cccgagtggc aggacgccat gacggccctc aaggattcgc tgcgcgtggc gggccagacg
     3421 acctacatcc gcatgcacga gcgccaggac cccgacggga aatgggagct gatcgaagcg
     3481 aaagtgtaac ggaacaacaa caaccggaaa gagacctgag aatgaaaaag acgaacgagg
     3541 acgcagtggt agtggcggcg gatgcgcggc cggtggatgt gaccgcgatg aagacgatca
     3601 tcggcgaaga cctgaaccgg gcggaggtgg gcgtgttctg catgctgcgc gcgggcgtgg
     3661 ggctgctggt ctacaaggag ctgagcgagc acggggagtg ggaggaccgg ctgctggagc
     3721 tgggaaaggg aaaatcaccg cgcaccctcc agatatacat gaagcaggcg cggacgctgt
     3781 gcgagcagta cgggatcacc cccgcgcagg cgtgggacga ggcgcggcac atcgacgcct
     3841 cgcaggtgac ggagctgctc ctggccgcgc caggcgagcc gcgccagatc ggcgccgggg
     3901 aacagccagc cgaagatccg cccgccaaga aagggcgcaa ggccaagaag agcaagcctg
     3961 cctccggccc tgacatgccg acgcagacat tcgcccagat gctgctggac ttcatggagc
     4021 agcggaaacg cgccaaggcc aaggacattg aacctaaaaa accgctgacc aagaaagaaa
     4081 agatagagac ggccatcgcc gaggccttcc gcgtggtgaa cctcacggcg gactgggtcg
     4141 cggacgggac atgggcgctg ctccctgacg aggagctgga aagcacgatg gcgggtctgc
     4201 gggcggccgc ggacaagatc cgcgacgagt tccggaaccg cagggcgaag atctgagggg
     4261 ggtgagatca tggcgacaac cgtgcttagt gtccgcatgc agagcccaga gttcgcggcg
     4321 ctcccgagcg aggagcgcgc gagcgtgctt gtctgggagg atgtcatgcg gcagatcgac
     4381 gcggccggca acaagctggc catgtgcagg gagtgggcgc agcgcaacgg gcaccggcgc
     4441 gggtggagcg ccgacaggat cagggcgaag tactacggct gggtggcgag cggccgcgcg
     4501 tggacgttcc tggtggactg ggcgcgcgtc cccaggacgc ccgcgccgga gcgccgcgga
     4561 ctggcgtctg ccttcaagcg ctactgcgaa gagaaccagc gggcgagcaa acccgcgtgg
     4621 cgggcgatgc tgcgcgacct gcggcgcggc gtccacatcc ccggcgtcgg gacatggcgc
     4681 gacgtgtggg ccgggcaatt cccgggcgag acgatcccgg agcgctgccc gtctgactgg
     4741 ataccgcgcg gatggcgcta cagcaacatg atccgcaagt acaagagcac gaagttcgag
     4801 ctggtggcga tgcgcaaggg cgctgcggcg gcgcggcagt tcgcgccggc cgtgtactcc
     4861 acgcgggtgg ggctgctgcc ggggcaaatc taccagttcg acgacatgtg gcatgacgtg
     4921 aagatcgccg tgcccggcgt caaccgcaag ctgctgcgcc cgctggagtt cgcctgcgtc
     4981 gatgtcgcga gcacgtacaa ggccgggtgg ggcatcaagc cgcagatcca gcgcgaggac
     5041 ggcagccgcg agggattggc agccggccag ttcaaagcgc tggtcgcgca tatcctctgc
     5101 aacgtcggct ggcatccgga cggctgccag atggtgatcg agcacggcac ggccagtctg
     5161 aacgatgcgg atcaggcgct ggtcacacgt ctcacagacg gggcggttac gttccgcacg
     5221 tccggcatcc ttggcggaca ggtcttgcgc gggatgttca gggggcaggg acgcggcaac
     5281 ttcaaggtca aagcgctgat agagggcagc caccgccagc cgcactatga ggcggcgagc
     5341 cttccggccc agacgggcgg tctttcgcgc gttgatgagc ctgagcagat gtacgggctt
     5401 gaccgatacg ccgaccgtgt gctcgcggca tgggatcgcg tcccggaaga gcagcgcaac
     5461 ttgctgtggt tcggtggcgc gctgacgctt tcggcgtaca cgcgcatcat tgcggacatc
     5521 tacgacgcga tctacacgcg caccgatcac gacatcgagg gatgggagca gaacgaatgg
     5581 atgctcctgc agtggagcgt ggacggccac agccactggt acacggttga cgacatcccg
     5641 aagctcgatc cagagatgca gcagatcggg cgtatcgcgc tgaataaacc gggtctgacg
     5701 cggttgcgtc gcatgagccc gcatgaggtc tgggaacggc acaaggacgg gctgaaaagg
     5761 ctcccgcact gggcggtcgt tgatttcctg ggcgacgagt gcttccgccg tgtgcgcgtt
     5821 ggccacgacg ggctgatcgc gtttcaggat cgcgacctga ccggctcagc ggtcaagctg
     5881 cgtttcgcag gcgcggtaat gcagccagac ggcaccggtt gccgtctcgc tccgggcgcg
     5941 gaatacgggc tttacgtgct gcctcacgat ctcaccaagg ctgtgatcgt cgaggccggt
     6001 acgcgggctg tgctcggcat tgcgccggca tggtcggcgg tcgatccgct caatgccgcc
     6061 caggtcgctg taatgcagga ggcgcaggcc aagatgatcg cgctccagtc tcagggtgta
     6121 cgcgagcgcc atgccgagcg cggcgacgag ctggtggtca ggcgcgaaga gaacgatttg
     6181 ctgctggcag gcctcacaga gccgcgtcgg cagaagacca agaaacaggc taaacagaaa
     6241 aagcaggcgg cgggacaggc aatgcgtgcc cttgcgggta tgcgttccga cgtcaatgaa
     6301 agcgaggtag aagatgagtg gtaatatggc gcgggcgaat tatgacacgg atgtacgtga
     6361 agcgttgcag gcgttccagg acaagaacgg cctcacgctc aaacagctcg gggcgcgtct
     6421 cggaaagaat gaggggttcg tctcgaagta ccttaacggt gtcggagagg gaaacattgc
     6481 agcgtttgaa aagcgcgtca aggacatgct ggagcgcgag tcgcgcaagc gcacatggga
     6541 gagcgtgtgc ttccacacgg acgcggtgga agcgtgctac acggtctttg atttggtgcg
     6601 cgaagcaagc gacataggtt tgatcacgag cgaggccgga attggtaaaa gcatggccgt
     6661 gcaccaatac atgatcgagc acaagaccgt gatcggcttc accgccatcg aggggagcgg
     6721 cagccaatac gatgtgcagc gcggcattgc cagttgcctc gatatgcgca agttcaaccc
     6781 gaggtcatgc cgcctttcgg attacctgcg cgaaaagctc gcagggtcag agcgtctggt
     6841 gatcattgat aacgcgcagc gcattttcct ctccggtctg cggtggatca tggatttcca
     6901 cgacgcgacc ggtgtctcgt tttgcctggt cggcaaccca gatgtcatgg aacgcctgga
     6961 ggggcgcgac cagctcgttt cgcggatcgg cataaagaaa gacatcacaa gtgcgctcgg
     7021 aaatgaaaag tggattgatg acgcagccga ccgcatggtc gcggcgatgt ggccgaaggc
     7081 cgcatcagag atcgcgatcc tcgcacacga ggccgcgcgg aaatctgggc acttacgcac
     7141 attgaacaag cagcttcgca tcgcgatcag gctctgcgag acagacatgt accgtaaccg
     7201 ttatgccaag tcattcgtgg tcgcacgcga cctgatcggc gcaggcgacg acgaggattg
     7261 atagaggcag cgatgtttta gcggagttgc agccccgcca aacgaaacag cgttcagccc
     7321 gctataaccg atgccgcgca ccgctgccag tggcagattg agattgcgtg gttattatct
     7381 cacaagaggt gacatgtgaa aagtcccttg gcatacatgg gcggtaagag ccgcatggtt
     7441 aaaaccatag tcccgatgat ccacaaaatc ccgcatacgt gctattgcga gccgttctgc
     7501 ggagccgcgt gggtgttatt cggtaaggat ccggatgtgt cagagtgcga agttataaac
     7561 gatgcggacg gcgagttggt gtcattatgg agcggcccga gacgctgaca gaccttgagc
     7621 gcgcggctag gtactactac cttcagaggc tttcgtttgg tgggaaagtc aagggccgca
     7681 cattcggcta cagcgcgaca tccgcgccca agttggacat aggctcaatc caatctgatc
     7741 tgctggatgt tcacatgcgt ctcgcccgtg tgacgattga gcacctcgac ggcatggatt
     7801 gcatccgacg ctacgatcgc aaggagtcgc tgttcttcat cgacccacca taccttgacg
     7861 tgaccggtta cgaggtgccg tttccaaagg atcgttacaa tgaactggcc gaacttctgt
     7921 ccagcgtaaa gggccggttt attctcacac ttaacaacca cccgcagatc cgcgaaacgt
     7981 ttaagcgttt caagatatca gaggttcaga cgagttactc gaccggcctc gaagcgaagc
     8041 aacgtgtgac ggaactgctc ataacaaaca ggtgatctat gctcacacca gcacagcgga
     8101 ctaagttttt tcaactcgcc agaaaagcct gggcaacggt ctccccagac agccctttcg
     8161 acgattggcg caaacagatc atgatcgatg ccggttattc cccatctgta tctcaagtcg
     8221 atcatgtatg gggctacgag aatcttatgc tgcgtttcgc cgtgctggct tacgacgact
     8281 ccgcaatagg gtattggagc gcatgcgtcg agcgtcgtct caggtgggtt ttgaagggcc
     8341 ttgcaaccga cttgcaatat ctccagacga ccggcgtcgg agaggagtac attgccggga
     8401 tctatgccca ggccggcatg ttgccagccg attttgagga cgcccctgtc cagcacctgt
     8461 ggtgttgcct gcagatcctc gatacgcgca ttcgcagtct gtgtaagcgc gacgggatcg
     8521 cgctgcactt gcttcctacc gcaggccccc cgtggcaatt ccgtggcgtt cgcgcggccc
     8581 gctacgccgc gtttgtggcg ctttccgctg acagggctag gcgtgatgcc ggtctaccca
     8641 ccgacgcgac agcggaacct tctggtgcgc ctgtgcgcga tgttgcagcc actgcctgat
     8701 ttgtgtgtca aacatagggc actccgcccc gttttagcct cgatctgtgt cacaccagat
     8761 cgagggcttt tttctgcgta agtcgttgat tttccgtgtc ttgccgcgtt tttccgcgtt
     8821 gtgtcagagc ggctcgtggt gcacgagcac gaatgcgctt ttcctcggcc tttgcacttc
     8881 cgagagaagc cgccgactcc tgctcgaatc gaaagaccat cctgatcctg ctgtcagaga
     8941 cagtgtgcgt cttgctttgg cgcggcgcgg cgacagcgac tatgcaagac agttcattca
     9001 gcagtatcag gccagcccga tcagccgcaa gctgacagtc gcactcgcca aggatcgcga
     9061 ggcaacaaaa acggttcgtc gcctcgagta tattgggacg ccggatgctt tgacggcttt
     9121 gttcgatggg gctgcatcgc atgcgcttgc cgaggagccg ttctctggat caaacagggt
     9181 cattgttcac agtactacgt tgcagaactt tcgtgacttc atgactgctc tgggagtcag
     9241 cattcccgac acggtgtcaa ccggacgcga actagcaaac tggtggaaag agaaccgaga
     9301 gcgccttgtc gcgctgatgc ggcagaagga atcaacactt cctcggatca gaggaacacg
     9361 cttcggtata ggctatttat aaagtggact gtcaatcgca actcaagtga ggtagaaaag
     9421 acagaaaaat gaaattacga acacatgttt tcggcggtat ggtgcttctt gcatgcagtc
     9481 ttcatctgtt tgcagaggta gaaaatgact ccatacagta cattgacgcg aaaagtgttc
     9541 cggtgattcg cttaaatgat gagcgccaga aaccctttgc ggaactttgg gacaaataca
     9601 aggcttcttc atcggccgcg tataggcgcg tgatcgtatc cttgatgaca gaacgtgaaa
     9661 acatggaggt caccgattct attgattgtg agatctgctc ttcttttgca gagaaccaga
     9721 ctgctgacgc ggctttgctc ctcgggatgt gtaatagcgg tcgcagtaga gacttgctca
     9781 ggcaacacac ggaggcaa
//
LOCUS       DAOSRB010000106         9667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6808_length_9667_cov_6.357678, whole genome shotgun sequence.
ACCESSION   DAOSRB010000106 DAOSRB010000000
VERSION     DAOSRB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6808_length_9667_cov_6.357678"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLZ74_07005"
     CDS             complement(<1..595)
                     /locus_tag="PLZ74_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOR98107.1"
                     /translation="MNRGLVVGMISLTLMGVRAETWVPEKMAESLHMGRRLEVFTNEV
                     RAAWGYAAPQKDAFLVLHPKQERKCAPLYVVLHSAGHDAFSCLACTRTPGNHDIYHAP
                     EEFFALYLDCRGNKGDWWWGIQRTKGPDIGPTEKRVIDTVKWVIEQYAVDPERVYLCG
                     NSMGGSGALGIGMRHGEIFAAIKANVPARVEHVSSRMG"
     gene            814..890
                     /locus_tag="PLZ74_07010"
     tRNA            814..890
                     /locus_tag="PLZ74_07010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:848..850,aa:Val,seq:gac)
     gene            1044..3134
                     /locus_tag="PLZ74_07015"
     CDS             1044..3134
                     /locus_tag="PLZ74_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98108.1"
                     /translation="MHKKTMTSGALALAMVLAGHAFAYETVMIPTLSPPNIVDGDLSD
                     PCWKKAYASAPFTAIKPGDKITEETRAYLFCTADTLYIGAKCSFTDYALREKIVNDKS
                     KSGFAGDCIELFLDPGDTGNYAHIAINVAGNVTSVGMVAPFRCSVQLYQTYWMMEAEI
                     PFAAIKLDAQSYNANWRINVARGNYKKGESSSWSKLENGVFGEPASFNSVNGIVADLV
                     AIMKAQKDAARKDFEIVFDRVVYTDQADVKATLDFLYTKSIAGFTTKATIVDATGKTV
                     LEHNAPLTAFHADFAFPLKGFADGKHVATIAIVDAAGKTIKSGEKTFYKVPPMRDSDN
                     VDRYTIRNHCVYRNGRFFFPIITWKLTSSDFASLEQWRSQSEAVLKEMAEHGFTTGMT
                     TDYSFPEEDLDVRRKTKSIAAWNQKITKSLHDAGVTFEDQCRMAKKYGINVIATSPYR
                     PKSPYATDCFVEHVARIRRTPNVMLWHTADETDGQVEYNTLLNRLYHELDPTRLTWLN
                     VINAVSANKDAADILSTDPYPIPNGRVTAVAAHADRLVANTKGRPQVGRWLWIQNFGG
                     EGGWTRPPTPAEVKCMSMLAINHGVKGLAFFTWAPLSQRNGVRQHPEGMSMFKAFSPF
                     LQKWTPALCQGEVKFQGRQGVLDVLAVEFEGRKVLSVVNIENNPVAAAPVEIPGFCDK
                     KMTLEGYGISVEEL"
     gene            3144..3941
                     /locus_tag="PLZ74_07020"
     CDS             3144..3941
                     /locus_tag="PLZ74_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98109.1"
                     /translation="MKTRIRPFYFLHLASSFAFVLSANALTVTLSADPELPAPNILTN
                     GFFRFDEGLKGWANSHSNEVCCIEMEGRGLSFFTKDKKSVWRSIPTSVVKPGRTYILS
                     CEIKPSSKVNTHTFSNGGSGLGCGLTYWSEDWKRAVSLCAYTDGPDRWFRTYSQPVTM
                     PDWIIHGQMTVGLAYSGGSGYVDNIELVEAYSELTICAESQAAIRQVKVVDHNFRTVY
                     DSGVLNNGAKTWTHRLQIEAAYKYAVFAVDAVGNVSVVHYPAKKAQE"
     gene            4203..7457
                     /locus_tag="PLZ74_07025"
     CDS             4203..7457
                     /locus_tag="PLZ74_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOR98110.1"
                     /translation="MGMTTMKFVMGAALATFVSFSHAATTYYVNGTSGNDANDGTSAA
                     PFKTIGKALTVAIDNDRIEVAPATYKITASLLVDKAVEIVGTGAEASDTYVLSGRENS
                     SFSGRPLVVSNALAKVANIKFRGGYGRYSDGIYPANCAWIYAGTVTNCVFADSWRVNK
                     GYGGVYAQTEDAYVVDCEIYGHFNVDGAGSNQDGIFCAAGLTLHNGTARGCYIHDNVS
                     RQNTGGVLLSGANAKLLDSSVIGNVGCGYDRVNGLTPWPGSGAGGIMIRNANAVVSNC
                     VVSANISGERMTTGSDNHYRAGGIWFASAGKVYNTVVIGNTSASEGGGVWGSGYLYNC
                     VIADNVAQFRTGGGVYGKAFLYNCTVTGNDSLLDGGVDGVTGSDANNKATVENGIVWG
                     NGTLDSANVTLTATYTEDPKFLDAAAGDYRLDIGSPCIDTASVPSVMLPETDAAGRPR
                     VANGALDYGALEAAPNFRVVGDPETFGVPATPAFGEHPDTTVGAVNASVPSPYTDATG
                     LTNVVCTGYTYVAQDNANNVTTNEGADTAYAAAYARLGTLTWHLLAEYKQTVTNRGNG
                     AGLVTGSGFVTRGATFTATATPAAGYRFWRWEGAIGDNDPTSPTLTLVSDFARTVTAV
                     FSRDWHVDGVTGDDGNPGTSTAPFKTIHQALAVADDGDTVYVAPATYGFDPDHQALVI
                     TNAIALVGTGASRTDVKVSYKASTENWTGFGGKRLLYVDNKDAKVQHIDFAGPDNKYN
                     RSGNIDGSTAYINAGLLSDCVFRDAFVDIAAGCGVYLNTEDAVAEDSLFSGFGSVNGY
                     DKGGVALTIVNGLARRCEIINNNGRQNGPVGLSGAKARLFDSIITNNQSCAAHKHTET
                     TWGGPRSGGGVWIRNAGAVVSNCVIAANRTNLPQGGLKTVCTGGGIVFAAAGSVYNTV
                     IRDNYAYLEGGGVYGVGNLYNCLIADNESEAGQGGAVSGAARLYNCTVVGNDSQLTDG
                     TDGISGDATLLNCIVWNNGAKNYDTAAMTYSCSSPKPTGEGNRDSDPQFRSSLSRGLY
                     CIASDSPCAKAGVWQDWMPGLKYLNGVPMPSEPGDVPMGCYAPYQIRGLIMEVR"
     gene            7734..9509
                     /gene="iorA"
                     /locus_tag="PLZ74_07030"
     CDS             7734..9509
                     /gene="iorA"
                     /locus_tag="PLZ74_07030"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOR98111.1"
                     /translation="MSQRVLLSANEAVAWAACKAGCAVASAYPGTPSTEILENVAKFK
                     ASIYCEWAVNEKVGMEVAIGASISGARALTAMKHVGLNVAMDPLMTYTYVGANGGLVI
                     ITADDPGMHSSQNEQDNRTLVRFARAVLFEPADSQEAFDMVIEAFEVSERYQVPVFVR
                     LTTRTSHTSTVVDVGDDFAPGHPPRKPYVKDIRRYIPVPMFARPQRLKAQERTAVLRD
                     LACASPFNRIEAGDTRFGIITSGVTYQYVKEVFPEFAVLKLGFSNPFPAERVREFAAS
                     VERVLVIEELDPFIEEQVRALGIPVVGHTTELNMFEMNPLRLMTLRNELLGDITLPEV
                     RRADAGLPTRPPVLCAGCSHRGVFYTLAKLGATVTGDIGCYTLGAFPPLNAMDTTICM
                     GASVGNAYGMEKAGKGGRLCAVIGDSTFFHSGMTGILNVLYNGGRTTTVVLDNRITGM
                     TGHQDNPGSGKTLCGDPAPVTDIAEVAKAMGFRRVVKVDAYDLKTLEKVLAEELDADE
                     PSVVVAGGPCRIAAKLMNAGTFEVDAEKCKACGACFKLGCPAIERGETVVEGKRYKAH
                     IDPVQCSGCDLCPQVCKFDAIKRVR"
BASE COUNT         1971 a         2821 c         3031 g         1844 t
ORIGIN      
        1 accccatgcg gctggagacg tgctcgacgc gtgcgggcac gttcgccttg atcgcggcga
       61 aaatctcgcc gtggcgcata ccgatgccga gtgcgccgct gccgcccatt gagttaccgc
      121 acaggtagac ccgttccgga tcaaccgcat actgctcgat cacccacttc accgtgtcga
      181 tcacacgctt ttccgtgggg ccgatatccg gtcctttggt tcgctggata ccccaccacc
      241 agtccccctt gttgccgcgg cagtcgaggt acaacgcgaa aaactcttcg ggagcatggt
      301 agatgtcgtg gttgccgggc gtgcgtgtgc aggccagaca agagaaggcg tcatgaccag
      361 ccgagtgcag caccacatag agcggcgcac acttgcgctc ctgcttggga tgcagtacga
      421 ggaacgcgtc tttttgcggt gccgcataac cccacgctgc ccggacctca ttcgtgaaaa
      481 cctcgagcct gcgccccatg tgaagcgact ccgccatttt ttcaggcacc cacgtctcgg
      541 cgcggacgcc catgagcgtg agtgatatca tcccgaccac aagacccctg ttcatctttt
      601 tcccctctct cccgaacgcg ttgtcccccc cgctaccgca taccggcaca tgttccgcgg
      661 gcccggactc cgtttgttcc atgtaatatc atattcccgt gtttcccgga acggttttcc
      721 gtccggccga cagggaggaa atgcgaaagc cgggttcccg gcggttgaca gggaacatcg
      781 catcgtttac actacttccg ttttttccgc ttcgggggat tagctcagtt ggttagagca
      841 cttgcttgac atgcaagggg tcggtggttc gagtccacta tctcccacca gatttcagcc
      901 ccgtttttat tggaaaagcc caatgaaaac ggggttttta ttttttgctt ttcgccccat
      961 cgacctcacg aacacaaggg gttgaggagc ggtgcagggt agtgtaagat atagagaatc
     1021 aatcaggaca tgaaagggag acgatgcaca agaagacgat gacgtccggc gcgctggcgc
     1081 tggcgatggt gctggcgggg catgcgttcg cctatgagac ggtgatgatc ccgacgcttt
     1141 ccccgccgaa catcgtggac ggcgacctgt ctgatccttg ctggaagaag gcctacgcga
     1201 gcgcaccctt tacggccatc aagccgggcg acaagatcac ggaggagacg cgcgcatacc
     1261 tcttctgtac ggctgacacg ctttacatcg gcgcgaagtg ctcgttcacc gactacgcgc
     1321 tccgggagaa gatcgtcaac gacaagtcga agagcggctt cgcgggcgac tgcatcgagc
     1381 tgttcctcga cccgggtgac acgggcaact acgcgcacat cgccatcaac gttgcaggca
     1441 acgtgacgag cgtcggcatg gtcgctccgt tccgctgctc ggtgcagctc taccagacgt
     1501 actggatgat ggaggccgag atcccgtttg cggcgatcaa gctggacgcg cagagctata
     1561 acgccaactg gcgcatcaac gtcgcgcgcg ggaactacaa gaagggcgag tcgtccagtt
     1621 ggtcaaagct cgagaacggt gtcttcggtg aacccgcctc attcaacagt gtaaacggca
     1681 tcgtcgcgga cctcgtggcg atcatgaagg cgcagaaaga cgccgcgcgc aaggacttcg
     1741 agatcgtatt cgaccgcgtc gtctataccg accaggcgga cgtgaaggca acgctcgact
     1801 tcctctacac gaagtctatc gcgggcttca ccacgaaggc gaccatcgtt gatgccaccg
     1861 gcaagaccgt cctcgagcac aatgcgccgt tgacggcgtt ccacgctgat ttcgcgttcc
     1921 cactcaaagg tttcgcggac ggcaagcatg tggcgacgat cgcgatcgtc gatgcggcgg
     1981 gcaagaccat caagtcgggc gagaagacgt tctacaaggt tccgccgatg cgggattccg
     2041 acaacgtcga ccgttatacg atcaggaacc actgcgtcta tcgcaacggc cggttcttct
     2101 tcccgatcat cacgtggaag ctgacgtcgt cggacttcgc atcgcttgag caatggcggt
     2161 cgcaatccga agcagtgctc aaggagatgg cggaacacgg ttttacgacg ggcatgacga
     2221 cagattacag ctttcccgag gaggatctcg acgtccgccg caagacgaag tcgatcgccg
     2281 cctggaatca gaagatcacg aagtcactgc acgatgcggg tgttacgttc gaggaccagt
     2341 gcaggatggc caagaagtac ggcatcaacg tcatcgccac ttcgccttat cggccgaagt
     2401 cgccctacgc gacggattgc ttcgtcgaac atgtcgcgcg catccgcagg acgccgaacg
     2461 tgatgttgtg gcatacggcc gacgagacgg atggacaggt cgagtacaat acactcctga
     2521 accggctcta ccacgaactc gacccgacgc gtctgacgtg gctgaacgtc atcaatgccg
     2581 tttcggcgaa caaggatgcc gccgacatcc tctcgaccga tccctatccg attccgaatg
     2641 gacgtgtgac ggcggttgcg gcacacgccg accgtctcgt cgccaacacg aagggccgtc
     2701 cacaggtcgg tcgctggctc tggatccaga acttcggagg cgagggcggc tggacgcgtc
     2761 cgccgacgcc ggccgaggtg aagtgcatgt cgatgctggc gatcaatcac ggcgtcaagg
     2821 gcctcgcctt cttcacgtgg gcgccgctgt cgcagcgcaa cggtgtccgc cagcatccgg
     2881 aggggatgag catgttcaaa gcgttctctc cgttcctgca gaagtggacg ccagcgctct
     2941 gccagggcga agtgaagttt cagggccgac agggtgtcct ggacgtgctc gcggtcgagt
     3001 tcgagggacg caaggttctc tctgtcgtca acatcgagaa caatcccgtc gccgccgcac
     3061 cggtcgagat tccgggcttt tgcgacaaga agatgacgct cgaaggctac ggtatttccg
     3121 tcgaggagct gtgagaaacg aaaatgaaga caagaatcag acctttttac ttcctgcatc
     3181 ttgcctcttc cttcgcgttc gtgcttagcg cgaacgcgct gaccgtcacg ctctcggccg
     3241 atcccgagct gcctgcgccc aatatcctta cgaacggatt cttccgtttc gacgagggac
     3301 tcaagggctg ggcgaacagc cactccaacg aggtctgttg catcgagatg gaagggcggg
     3361 gattgtcgtt cttcacgaag gacaagaaat ccgtctggag aagcataccg acctcggtgg
     3421 tcaaaccggg gcgcacctac atcctctcgt gcgagatcaa accgtcgtca aaggtcaaca
     3481 cccacacctt ctcgaacggc ggcagcggcc tcggctgcgg actgacctac tggagcgagg
     3541 actggaagcg cgcggtcagt ctgtgcgcat ataccgacgg tcccgacaga tggtttcgca
     3601 cgtactcgca gccggtcacc atgcccgact ggatcatcca cggccagatg acggtcggtc
     3661 tcgcctattc cggcggttcg ggttacgtgg acaacatcga actcgtggag gcgtactcgg
     3721 aacttacgat ctgcgcggag tcgcaggcgg cgatccgtca ggtcaaggtc gtggaccaca
     3781 acttccggac ggtctacgat tcgggcgtgc tgaacaacgg cgcgaagacg tggacgcacc
     3841 gcctccagat cgaggcggcg tacaagtacg cggtcttcgc ggttgacgcg gtgggcaacg
     3901 tctcggtcgt gcattatccc gcaaagaagg cgcaggaata aaccggcgga cgccatgcgc
     3961 gcgactttct gccggagggc tgtgcccgcg tgttgaagat cgggtatgac gcgggggcgc
     4021 cccatgttgc cgactcgaca tgaggcgtac cgcagaaggc cggtgaccgt cgtttcgatg
     4081 tcctgatttg gcgatagttg acggcgaaca gggttgtttg atataaaagt aaagtaaagt
     4141 gtttcggtag aacctatgcg agcaagccgg atgagttgat gtttgcagaa aggactcagg
     4201 ctatgggtat gacgacgatg aagttcgtaa tgggggcggc gttggctacg ttcgtttcgt
     4261 tctcacacgc cgctacgaca tattacgtca atggcacgag cggaaacgac gccaacgacg
     4321 gtacttcggc tgcgccgttc aagacgatcg gcaaggcact gaccgtcgcc atcgacaatg
     4381 accgcatcga agtcgccccg gcgacctaca agatcactgc gtcgctcctc gtggacaagg
     4441 ccgtcgagat cgtcggtacg ggcgcggaag cgagcgacac ctatgtgctt tcgggtcggg
     4501 agaattcgtc cttcagcggc cgtccgcttg tggtgagcaa cgcgcttgcg aaagtggcga
     4561 acatcaagtt ccgcggcggt tacggccgat actcggacgg catctacccc gcgaactgcg
     4621 cgtggattta tgcgggtacg gtgacgaact gtgtttttgc cgactcgtgg cgcgtcaaca
     4681 agggttacgg cggcgtttat gcacagaccg aggatgcgta tgtcgtcgat tgcgagatct
     4741 acggccactt caatgtggac ggcgctggca gtaatcagga cggaatattt tgtgcggcag
     4801 gcctgacgct ccacaacggt acggcgcgcg gttgctatat tcacgacaac gtctcgcgcc
     4861 aaaacacggg cggcgtgttg ctttcgggcg ccaatgcgaa gctcctcgac tcgagcgtca
     4921 ttggaaatgt cggatgcggc tatgaccgcg tcaacggcct gacgccctgg ccgggctctg
     4981 gcgcgggcgg tatcatgatc cgcaacgcga atgcggttgt ctcgaactgt gtcgtttcgg
     5041 ccaacatatc gggcgaacgc atgacgacag gctccgacaa ccactatcgg gcgggcggca
     5101 tctggttcgc ctcggcggga aaggtctaca acacagtcgt catcggcaat acgtccgcga
     5161 gcgagggcgg cggcgtgtgg ggctcgggct atctctacaa ctgtgtgatc gccgacaacg
     5221 tggcgcagtt ccgcacaggc ggcggagtct acggcaaggc gtttctctac aactgcacgg
     5281 tgacagggaa cgactcgctg ctcgatggcg gtgtggatgg cgtgacaggc tcggatgcga
     5341 acaacaaggc aacggtcgag aacggcattg tctggggcaa cggcacgctt gattctgcga
     5401 acgtgacact gacggcgacg tatacggaag acccgaagtt cctcgacgcg gcggcgggag
     5461 actatcgcct cgacatcggt tcgccctgca tcgacacggc aagcgtcccg tcggtgatgc
     5521 taccggaaac ggacgccgcg ggccgtccgc gtgtggcgaa cggcgcgctc gactacggtg
     5581 cgctggaggc ggcgccgaac ttccgcgtcg tgggtgatcc cgagaccttc ggcgtaccgg
     5641 cgacacccgc attcggtgaa catcccgata caacggttgg cgcggtgaac gcctcggttc
     5701 cctcgccata tacggatgcg acggggctca cgaacgttgt ttgcacgggc tatacctacg
     5761 tggcgcagga caacgccaac aacgtaacga caaacgaggg tgcggacacc gcgtatgcgg
     5821 ccgcgtacgc acgcctcggc accctgacat ggcaccttct ggcggagtac aagcagacgg
     5881 taacgaatcg cggcaacggc gcgggactgg tgacgggatc cgggttcgtc acgcgcggcg
     5941 cgacgttcac ggcgacggct accccggcgg cgggctatcg tttctggcgc tgggaaggtg
     6001 ccatcggtga caacgatccg accagcccca cgctgacgct tgtgtccgat ttcgcgcgta
     6061 cggtcacggc ggttttcagc cgtgactggc atgtcgatgg cgtaacgggc gatgatggca
     6121 accccggcac gtctacagcg ccgttcaaga caatccatca ggcgctcgcg gtggcggatg
     6181 acggtgacac tgtctacgtc gcgccggcca cctatggctt tgaccctgac catcaggcgc
     6241 ttgttatcac caacgccatt gcgctggtcg gtacgggagc cagccgtacg gacgtcaagg
     6301 tgtcctataa agccagcaca gaaaattgga cgggcttcgg cggcaagcgc ctcctctacg
     6361 tcgacaacaa agatgcgaag gtccagcaca tcgacttcgc cggcccggac aacaaataca
     6421 accgctcggg caacatcgac ggctcgacgg cctatatcaa cgcgggtctg ctaagcgact
     6481 gcgtcttccg tgacgcgttt gtcgacatag cggcgggatg cggcgtctac ctcaacacgg
     6541 aagacgcggt tgccgaggat tcgctgttca gcggcttcgg cagcgtgaac ggttatgaca
     6601 aaggcggtgt ggcgctgacg attgtgaacg gccttgcgcg gcgctgcgag atcatcaaca
     6661 acaacggtcg ccagaacgga ccggtcggct tgagcggcgc gaaggcgcgt ctcttcgact
     6721 cgatcatcac caacaaccag agctgcgctg cccacaaaca tacggaaacc acatggggtg
     6781 gtcctcgaag cggcggtggc gtgtggatcc gcaatgcggg cgcggtggtc tcgaactgcg
     6841 tcatcgcggc gaaccgcacg aaccttccgc aaggtggctt gaaaaccgtt tgcacgggcg
     6901 gcggcatcgt gttcgcggcg gcgggctcgg tctacaatac cgtcattcgc gacaactacg
     6961 cttatctcga aggcggtggc gtgtacggcg tcggcaatct ctataactgc ctgatcgccg
     7021 acaacgagtc ggaggcggga cagggtggag ccgtcagcgg cgcggcaagg ctctacaact
     7081 gcacagtcgt cggcaacgat tcgcaactca cggacggtac ggacggcatc agcggtgacg
     7141 caacgctcct caactgcatc gtctggaaca acggcgcgaa gaactacgat acggcggcga
     7201 tgacatattc ctgctcgagt ccgaagccga cgggcgaagg caatcgggat agcgatccac
     7261 agttccgcag cagcctgagc cgcggccttt actgcatcgc cagcgattcg ccatgtgcaa
     7321 aggcgggtgt gtggcaggac tggatgccgg gcctcaagta cctgaacggc gtccccatgc
     7381 cgtccgaacc gggcgatgtc ccgatgggct gctacgcgcc gtatcagatc cgggggctga
     7441 tcatggaggt ccggtaagcg tcctcgggca gcgtgatccg gtgaatgccg cgcaagtcac
     7501 catagcttcc gcgtccataa ctcacccttc ccagtactga aacaatatgc tacattttac
     7561 atgtaattgg ttcaaatggt aaggggtggg tggttcgagt ccacgatctc ccgccagatc
     7621 aggatgtatt cggaaaaccg gaggaagcag gtttttttta ttttatgggg cagtgcgccc
     7681 gttccgggcc tcccgcgcga agcgctaccc ctatcgggga atggagacaa acaatgagtc
     7741 agagagtcct gctgtcggcg aatgaagcgg tggcgtgggc ggcctgcaaa gcggggtgcg
     7801 ctgtggcgtc cgcttatccg ggcacgccaa gtacggagat tctggagaac gtcgccaaat
     7861 tcaaagcgtc gatctactgc gaatgggcgg taaacgagaa ggtcggcatg gaggtcgcga
     7921 tcggcgcgtc gatctccggc gcgcgcgcgt tgaccgcgat gaagcacgtc ggcctgaacg
     7981 tggcgatgga tccgctcatg acctacacgt atgtgggggc aaacggcgga ctggtgatca
     8041 tcacggctga cgatcccggg atgcacagtt cgcagaacga gcaggacaat cgcactctgg
     8101 tgcgcttcgc gcgcgccgta ttgttcgagc ctgcggacag ccaagaggcc ttcgacatgg
     8161 taatcgaggc gttcgaggtc tcggagcgct atcaggtgcc ggtgttcgta cgtctgacca
     8221 cgcggacttc gcacacctcc accgtggtgg atgtggggga tgacttcgcc cccggccatc
     8281 cgccacgcaa accgtatgtc aaagatatcc ggcgctacat cccggtaccg atgttcgcgc
     8341 ggccgcaacg cctgaaggcg caggagcgta ctgcggtcct gcgtgatctg gcctgcgcgt
     8401 cgccgttcaa ccggattgag gcaggcgaca cgcgtttcgg gatcatcacc tccggggtga
     8461 cctaccagta cgtgaaagag gtcttccccg aattcgccgt gctgaaactg ggcttttcaa
     8521 atccgttccc agccgaacgg gtccgggagt tcgcggcaag cgtggagcgc gtactggtga
     8581 tcgaagagct ggacccgttc atcgaggagc aggtccgggc gttgggcatc ccggtcgtgg
     8641 ggcacacaac cgagttgaac atgttcgaga tgaatccgct gcggctcatg acgctgcgta
     8701 acgaattgct cggcgacatc acgctgccgg aagtccggcg ggcggatgcc ggactcccca
     8761 cgcggccgcc ggttctgtgc gccgggtgca gccaccgcgg ggtgttttac acgctcgcca
     8821 agctcggcgc cacggtgacg ggcgacatcg gatgctacac cctgggggcg ttccccccgc
     8881 tcaacgccat ggacaccacg atctgcatgg gcgcctccgt cggcaacgcc tacggtatgg
     8941 agaaagccgg caagggcgga cgcctctgcg cggtgatcgg tgactccacc tttttccatt
     9001 ccggcatgac cggtatcttg aatgtgctgt acaacggcgg caggaccacg acggtcgtgc
     9061 tcgacaaccg gatcaccggc atgaccgggc atcaggacaa tccggggtcg ggcaagaccc
     9121 tgtgcggtga tcccgcgccg gtcacggata tagcggaggt tgccaaggcc atggggttcc
     9181 ggcgcgttgt gaaggtcgac gcatacgatc tcaagacact ggaaaaggtg ttggcggaag
     9241 agttggacgc ggatgagcct tcagtggtgg tggctggcgg accttgccgc atcgcggcca
     9301 agctgatgaa cgccggcact ttcgaggtgg atgcggagaa gtgcaaggct tgcggcgcat
     9361 gtttcaagct gggctgccct gcgattgaaa ggggcgaaac ggttgttgaa ggaaagcgtt
     9421 acaaggcgca tatcgatccg gtgcagtgct ctgggtgtga cctgtgcccg caggtctgca
     9481 aattcgacgc gatcaaacgg gtgcggtagc cggtcacatt cttcttatgg gtaaaaaaaa
     9541 gacattcaaa cctaacgacg gacgatgttt tgttccgaag agcaagcgta agcctcgtga
     9601 tatcaagaac aagcttttcg aaggcgattg tctggagata ttgccgtttt ttcccgatgc
     9661 ctgcgta
//
LOCUS       DAOSRB010000107         9638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6843_length_9638_cov_5.532714, whole genome shotgun sequence.
ACCESSION   DAOSRB010000107 DAOSRB010000000
VERSION     DAOSRB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6843_length_9638_cov_5.532714"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1532
                     /locus_tag="PLZ74_07035"
     CDS             336..1532
                     /locus_tag="PLZ74_07035"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOR98112.1"
                     /translation="MGKQKTQVEHEIALRVGARIRQLREIQRISQIKLATEIGIRAGP
                     LGWIEKGKHLPSGRVLYRLAKQLNVRIDDLFQEQNVWEESAAARDGKQPVMLPPMDNG
                     INAGAEPLKSAHIICLSVAQALLKLETLCGVVKTSSLALTIPFAPTERGAEYLAGRLR
                     QDLGIGETVTHGYLEALESAGVRVIFLDMPEGCPTFSGYDPQNRNALIFVNSRLKKQP
                     ELQLFRLAFELGRVCWYVRETGEGQGSGGPLPENGAETLSEAEFARRFAAFFLMPARA
                     VQASVWQLGLSPVGWTWELLLRLKKRYGVSAQRFALRLQALGLSWTDKQKKTPSAYRF
                     KEEIEAFYDANGKSAEPGGTRPPLVMNGRLGDLLLCAERLKEKETAKAVHAVKRVLRQ
                     AGVRID"
     gene            complement(1632..2534)
                     /locus_tag="PLZ74_07040"
     CDS             complement(1632..2534)
                     /locus_tag="PLZ74_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOR98113.1"
                     /translation="MKVLFLFIDGVGLRPPATDNPVHSGICPTLCHLIEQHGVSIDAC
                     LEVTGLPQSATGQTALFTGVNASQIMGRHCEGFPGPTLRKLIEQGNLFMDLSRKGLRC
                     RFADAYLVDSVDELRGRRFKSVTTVMALTQPETISIQDDLMANQAVFHDITRKSLQEK
                     GLAIPLVTPPQAAEHLIEVALANDFTLFEFFQTDLAGHSCQYDQACETLRTLDLFLDM
                     AVRLCKTTGLLLILTSDHGNIEDMGTRGHTRNPVPLIALGPGADEIKNLASSLTDITP
                     RLTRLMVPGHTSMPRTAPPADSAQ"
     gene            2699..3895
                     /locus_tag="PLZ74_07045"
     CDS             2699..3895
                     /locus_tag="PLZ74_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOR98114.1"
                     /translation="MAKVLIIGAGGVGAVVAHKCAQVPEVFTDIVIASRTRARVDALA
                     ARLPRKVTTAQVDADNVPQTVRLLKRHRPDLVINVALPYQDLHIMDACLEVGVNYLDT
                     ANYEPPDQAKFEYKWQWAYHSRFKKAGLMALLGSGFDPGVTSVFCTYARKHFVPHVEE
                     IDIIDCNAGSHGQPFATNFNPEINIREVTAKGRFWEDGEWKETEPLSVCSSFTMPENL
                     GSLKVYLMYHEELESLARHIPELRRARFWMSFSDNYLKHLEVLRNVGMTRIDPVRFNG
                     VDIVPLQFLKALLPDPASLGPLTKGKTCIGCQVRGRNARGQRKTVFIYNICDHEECYR
                     EVQSQAISYTTGVPAMIGAKMMLTGQWRGKGVFNMEQFDPDPFMADLTVYGLPWTVLK
                     KAVFKK"
     gene            4322..5023
                     /locus_tag="PLZ74_07050"
     CDS             4322..5023
                     /locus_tag="PLZ74_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98115.1"
                     /translation="MSVGNIAITVAVALLLVGQSAQAASSVPVMINTSPARDGHWSRV
                     CTNTVLLSWEWRDESAVRAELTLDGLRGTQTVVFTRPATNWVWQVSATPVPSEEDVVD
                     LTLRFYDAQNAVIRVQQSRLSVLSGAYGETPVLLSDADKRWTHVRDAAVIPHDVRWIN
                     TTAPASNSRLSIRRQDGVAQTNLLPAAGYYGWKLRDGSWGYGIFDLALDFPGSAGLWE
                     ATLLRVPEGSIFSVK"
     gene            5134..6429
                     /locus_tag="PLZ74_07055"
     CDS             5134..6429
                     /locus_tag="PLZ74_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOR98116.1"
                     /translation="MTTTMKTALASLAACLALNSLAADISHVSVRQRWPWSKLVDIDY
                     VIANATQGVDIAVTAHNGSIPLVLGDNELSGDLYGVSGQGARRIVWDPTQSQYTNEEV
                     LSQFKVTLTPVTPPLYMIVDLTKEAGADKQIEYVYEEDLTNGLWGAWMRNPITNAVGE
                     AIVESVIWTGVTTNDLYKTDKLVLRRVPAGSFQQGDTQNGADNVTLTKNMYAGVFEVT
                     QKQWDHVMGTTGGTATQAKHTVSYYEIREDPANADDPAVNWPSNRIVNATSFMGRLRA
                     KTGIGDFDLPTEAQWEYVCRAKTTTVFNDGNTKAKYTGTEDNNNGNTNEHLNALGWYK
                     FSAPTPPTAAQLVGSKRPNAWGLYDMHGNVWEWCLNWGTTSAETGTDPDGADSGSKRV
                     FRGGSVYDSASYCRSAFRYQDPPGSRFFSLGFRLFRTLP"
     gene            complement(6556..8655)
                     /locus_tag="PLZ74_07060"
     CDS             complement(6556..8655)
                     /locus_tag="PLZ74_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOR98117.1"
                     /translation="MTKMNDTPIATAVAVLLLAIACASKVAADELQDCELKRLPLGSI
                     CPEGWLKHQLDKMTDGICGRLYEYGKFLNPTNGWISRAEQGELDNQRHWNEGTGWEEQ
                     PYWFRSFVKLAALTGNERMLGVCRDWTEKMLATRDPDGWYGPQPLKRVRLKDGTFMQD
                     IWGHMVMNEALLSWYDYSGDRRVMALLSGFCDFCLAVPEDRFIHPPQGNGYAPASGWL
                     PLIQKARAGDMISTLFRLYDVTKDAKFTRLSDKVWRRYYVKRESLFLNIHNVNFCQMF
                     SYPSIYARRTGDLALAEESEYWLKQHWMTWGQMPRCAFAADENCRVGCIDPRYGLESC
                     SWGEYVRSFQNLSDHRAETKWADRTEDVVFNHAPCAYTPDWNEVHYITACNQTVIDAR
                     TDHDYANHPPMNAYSHTEYRCCLYNAAMPFPLFTENLVKRARDGALVFWMYAPCSGKT
                     DGVEWKLDTRYPFRETARLAVNAAKPLVLRFRVPAWAKAFSVGGTAATPGVRMLDVKV
                     PAGGSTLDIAMKAECEFTPWARQNGISVDRGPLTYSVAIGERYSKVSVVKGDSGSVIW
                     VEGNNNRDADRVYTEIAPTTPWNYGIDVTAKPVWKECVWNDDCFVSSNACCELSVRCR
                     RIPEWTLQDNEPARLQLSPAYSSEPLETVRFTPLCCQRLRLSVLPQVTDNATLGYRWK
                     KVPPTTDRKNRAKIFSP"
     gene            complement(8816..>9638)
                     /locus_tag="PLZ74_07065"
     CDS             complement(8816..>9638)
                     /locus_tag="PLZ74_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98118.1"
                     /translation="IYQNYGEGGLYRGDNAAALGPAAPLIYPNGWYANSYSLAKGYCT
                     YVKVNGVETDPTTTGLDNDLNVISLRTYIDPVSASAFNCIHYNNKFGGAQYGEVMFYT
                     RPVSRPEAHRIETYLRNKWCREGYSTAGYEAAVAGKVMLGTNAMLKVVGGEPLTATGL
                     GGGGTVTGDVTLQNNAELTVVITDGVASSQTIEGELVLPVNGTIKVVGDFTGLAFGDS
                     DVFKSASIHASTDLSGWGRNLTQVVHPEKRNVSFRVVEGAIRMTVSKSGTTFILR"
BASE COUNT         1926 a         2751 c         3034 g         1927 t
ORIGIN      
        1 gttcggtgca gctctcgtac cgtcccgccg atctcttcac cggcgaggcc ggcccgtgga
       61 tgcggctctt ccgaaacgcc tatgcgtttg tgacgggaac agcggttcgg gcataagttt
      121 ttttcctacg ctccatctgt aaaaaatccc atattcttgt ttttttagat gattcccctt
      181 gcgcggatca ccttgaactg caacaatata atcactttgg tgaatgagtc gtttgcggta
      241 gcgggtgcgt aataccctta cggcatgcgg ttgcacggca gaatagtggt gaacgcggcg
      301 gaataaggtg cgaacataca agagaaaggt tccgtatggg aaagcagaaa acgcaggtgg
      361 aacatgaaat cgccttgcgg gttggagcga ggatccgtca gttacgtgag attcagcgta
      421 tttctcaaat taaactggcc acggaaatcg gtatccgtgc cggtccgttg ggctggattg
      481 agaagggcaa gcacctgcct tcgggacgcg ttctgtaccg gttggccaag cagttgaatg
      541 tccgcattga cgatctgttc caagaacaga acgtgtggga agagagcgcg gcggcgaggg
      601 atggcaaaca gccggtgatg ctgcctccga tggacaacgg gatcaacgcg ggcgcggaac
      661 cgttgaaatc ggcccacatc atctgcctat cggtcgcgca ggctctgctg aagttggaga
      721 cgttgtgcgg cgtggtaaaa acctcctcac tcgcgctcac gataccgttc gcgcccacgg
      781 aacggggagc ggaatatttg gccggccggt tgcggcagga tttgggtatc ggcgagacgg
      841 tgacgcacgg ttatttggaa gcgcttgaaa gcgccggagt gcgtgtgatc tttttggaca
      901 tgccggaagg gtgtccgacc ttcagtggat acgacccgca gaaccgtaac gcgttgatct
      961 tcgtcaacag ccgcttgaaa aagcagccgg agcttcagtt gttccgtctg gcgtttgagc
     1021 tggggcgggt ctgctggtat gtccgggaaa ccggagaggg gcagggttcc ggcggaccat
     1081 tgcccgagaa cggtgccgaa acgttatcgg aagcggagtt cgcgcgccgg ttcgcggcgt
     1141 ttttcctaat gcccgcacgt gcggtgcagg cctccgtatg gcaattgggg ctgtcgcctg
     1201 tcggatggac atgggaactg ctgttgcgtc tgaaaaaacg gtatggcgtt tccgcgcagc
     1261 gtttcgcgct gcggttgcag gcgctgggat tgagttggac tgacaaacag aagaagactc
     1321 cgagcgccta ccgcttcaag gaggagatcg aggcgtttta tgacgcgaac ggaaagtcgg
     1381 ccgagccggg cggcacccgt ccgccgctgg tgatgaacgg ccgtctcggc gatctgctgc
     1441 tgtgcgccga gagattgaag gaaaaagaaa cggccaaggc ggtccatgcc gtcaagcggg
     1501 tgttgcgcca ggccggggtg cggatcgatt gagagcctaa aagagcggga gacaagagac
     1561 gcaagacagg agtcgggggc gcgggcgtcg ctgcccgata cgaacgcggg ccgactgtcg
     1621 caagtcgccg tttactgggc ggaatcagcg gggggggctg tccgcggcat ggaggtgtgt
     1681 cccggaacca tcagacgcgt caggcgcgga gtgatgtccg ttagactgga cgcgaggttc
     1741 ttgatttcgt ccgcgccggg gccaagggcg atcagcggca ccggattgcg ggtgtggccg
     1801 cgtgtgccca tgtcttcgat attgccgtgg tcgctggtga ggatcagcag caaaccggtg
     1861 gtcttgcaga gtctcacggc catgtcgaga aagagatcca gcgtgcggag tgtttcgcat
     1921 gcctgatcgt attggcatga gtggccggcc agatccgtct ggaaaaactc gaacagggtg
     1981 aagtcattcg ccaacgcgac ctcgatgagg tgttcggcgg cctgcggcgg ggtgaccagc
     2041 ggaatggcca accctttttc ttgcagactc ttacgggtga tgtcatggaa gaccgcctga
     2101 ttcgccatca ggtcatcctg aatggagatc gtctcgggct gggtcagcgc catcacggtt
     2161 gttacggatt tgaaacggcg gccccgcagt tcgtccacgc tgtctaccag ataggcgtcc
     2221 gcaaatcggc agcgcagccc cttgcgcgag agatccatga acagattgcc ctgctcgatg
     2281 agtttgcgga gcgtgggacc gggaaagcct tcacaatgac gtcccatgat ctgcgaggca
     2341 ttgacacccg tgaacagggc cgtctgtccg gtggcgcttt gcggcaagcc tgtcacctcc
     2401 agacacgcgt cgatcgacac cccgtgctgt tcgataagat ggcacagcgt cgggcagatg
     2461 ccggaatgga ccggattgtc cgttgcgggt ggacgcagtc ccacgccatc gatgaacagg
     2521 aaaaggacct tcactttgct cttttgtatc caaacccggc ccggcatgca agcctcgaat
     2581 ccgaaaacct tttcgcgggc acctttccca gtgaaaagga tcgccttgtt gattgggatg
     2641 acctcccgct ccaaatggtg tatactctcc ccattcccaa ccacaaaagg atgatctcat
     2701 ggccaaagta ctgattatcg gcgccggcgg tgtcggcgcg gtcgtggcgc acaagtgcgc
     2761 gcaggttccc gaggttttta ccgacatcgt gatcgcttca cgaacccgcg cccgggtgga
     2821 tgcgctggcc gcacggttgc cgcgcaaggt caccaccgcg caggtggatg cggacaacgt
     2881 gccgcagacg gtccggctgc tgaagcggca ccggccggat ctcgtgatca acgtggcgct
     2941 gccctatcaa gacctgcaca tcatggacgc ctgtcttgag gtgggcgtga actatctgga
     3001 taccgccaac tacgagccgc cggatcaggc gaaatttgag tacaagtggc aatgggccta
     3061 tcatagccgc ttcaaaaaag ccggtctcat ggctctgctc ggttcagggt tcgaccccgg
     3121 cgtcacgagt gtgttctgca cctacgcccg taaacatttc gtgccgcatg tcgaggaaat
     3181 cgacatcatc gactgcaacg ccggcagtca cggtcaaccg ttcgcaacca atttcaaccc
     3241 cgagatcaat atccgcgagg ttacggccaa gggccgtttc tgggaggatg gcgagtggaa
     3301 ggagaccgaa ccgctttcgg tgtgttcctc cttcacgatg cctgaaaacc tcggttcgct
     3361 gaaggtctac ctgatgtatc acgaggagct ggagtctctg gcccggcaca tcccggagtt
     3421 acggcgcgcg cggttctgga tgagtttctc tgacaactac ctcaagcatc tggaggtgtt
     3481 gaggaatgtc ggcatgacgc ggatcgaccc cgtgcggttc aacggggtcg atatcgtgcc
     3541 gctccagttc cttaaggcgt tgctgccaga ccccgcctcg ctcggtccgc tgacgaaagg
     3601 caagacctgc atcggctgtc aggtccgggg acggaacgcc cgcggccaac gcaagacggt
     3661 gtttatctac aacatctgcg atcacgagga gtgttaccgc gaggtccagt ctcaggcgat
     3721 ctcgtacacc acgggggtgc cggccatgat cggcgcgaaa atgatgctca ccggtcaatg
     3781 gcggggtaag ggggttttta acatggagca gttcgatccc gaccccttta tggcggatct
     3841 taccgtgtac ggcctgccct ggaccgtgct taaaaaagcg gttttcaaaa aataaccgaa
     3901 aaaattggct atattcaatc cgttccgggg ggcgacaaac ggcagcccgg tggtggactg
     3961 gccgtcgacg ggttccgcgg ttcggccgtc cagtttcatg gggtgcgtcc gggccaagac
     4021 ggggatcagc gggttcgacc tgccgagact acggcttccg cgttgtgata actttgccat
     4081 aacgctgtag cgctaccctc gcctcggccc gtcggggggg ggcgcaggcg taggccggac
     4141 gtgtgttggc ggggtgcggt gttttcgacg gttcgggaca acgtccccct caaaaaacct
     4201 cgtacccttc gcgcctccgc gtgagaaaaa aaagagcaga actgaaatca actcgatggc
     4261 ttgacacgct tctggggatg cggtttacta ttatcaacaa tgatgattgg agggagggga
     4321 catgagcgta ggcaacatag cgataacggt tgcggtggcc ctgctgctgg tcggccagtc
     4381 ggcccaagcc gcctcatcgg tgccggtaat gatcaacacc tcgcccgcgc gtgatggcca
     4441 ctggtcgagg gtctgcacca acacggtgct gctgtcctgg gagtggaggg acgagagtgc
     4501 cgtgcgggcg gagttgacgc tggatggtct gcgcggcacg cagaccgttg tctttacgcg
     4561 ccccgcgacc aactgggtct ggcaggtcag cgcaaccccg gtaccgtcgg aggaggacgt
     4621 ggtggacctg actctgcgtt tttatgacgc gcaaaatgcc gtgatccgtg tgcaacagtc
     4681 ccggctaagt gtcttgtcag gcgcgtatgg cgaaacgccg gtgcttctgt ctgatgccga
     4741 taagcgctgg acgcacgtac gggacgcggc ggtgatcccg catgacgtcc gctggatcaa
     4801 cacgaccgcc ccggcttcga acagccgcct gtcgatcagg cgtcaggacg gggtcgcgca
     4861 gaccaacctg ctccctgccg ccggttacta cggctggaaa ctgcgggacg gctcatgggg
     4921 atacggtatc ttcgatctgg cacttgattt tccgggaagc gccggtctgt gggaggccac
     4981 gctcctccgc gtgccggagg ggtccatatt cagcgtgaag tgagaatcgg gaagttctaa
     5041 agtgtgtttg aaatcgggat tttgactcgg gatggttcag gtcgtctcaa gtcgatgcat
     5101 gtcggtcaaa gaaagaagaa tggagaaacg gcaatgacaa caacaatgaa aacggcattg
     5161 gcgagtttgg cggcgtgtct ggcgttgaac agcctcgcgg ctgacatcag ccatgtcagc
     5221 gtgcgtcagc gttggccgtg gagcaagctg gtggacatcg attatgtgat cgcgaatgcg
     5281 acgcaggggg tggatatcgc ggtgacggcc cacaacggct cgataccgct cgtgctgggc
     5341 gacaacgagc tctccggcga tctttacggt gtttccgggc agggcgcgcg ccggatcgtc
     5401 tgggatccga cgcaaagcca gtacaccaat gaagaggttt tgtcacagtt caaagtgacc
     5461 ctcacgcctg taacgccgcc gctctacatg atcgtggacc tgacgaaaga ggcgggagcg
     5521 gacaagcaga tcgagtatgt ttacgaagag gatctcacga atggcctctg gggcgcgtgg
     5581 atgcgcaatc ccattacgaa tgcggtggga gaggcgattg tggaatcggt catctggacc
     5641 ggagtgacca ccaacgacct ctacaagacc gacaagctgg tgctgcggcg cgtaccggcg
     5701 ggctccttcc agcaggggga cacacagaac ggagccgata atgtgaccct cacaaagaat
     5761 atgtatgccg gtgtcttcga ggtcacgcag aagcagtggg accatgtcat gggcacgacg
     5821 ggcggtacgg ctacgcaggc taaacacacg gtctcctatt atgagatccg cgaggatccg
     5881 gcgaatgcgg acgatccggc ggtgaattgg ccatccaaca gaatcgtgaa tgcgacatct
     5941 tttatgggta ggctgagggc gaaaaccggt atcggagatt tcgacctccc gaccgaggcg
     6001 caatgggagt acgtctgccg ggccaagacc acgaccgtgt tcaacgacgg caacacgaaa
     6061 gcgaaataca ccggaaccga ggacaataac aacgggaaca ccaacgagca tctgaatgcg
     6121 ttgggatggt acaagttcag tgcccccacg cctccgacag ccgcacaact ggtcggcagt
     6181 aaacgcccca atgcctgggg actctacgac atgcacggca acgtgtggga gtggtgtctg
     6241 aactggggca cgacgagcgc agaaacgggg acggatccgg acggggcgga ttcaggttcg
     6301 aagcgcgtgt ttcgaggcgg aagcgtgtac gactccgcgt cgtattgccg ttcggcgttc
     6361 cgttaccaag atcccccggg cagtcgcttc tttagccttg gcttccgcct tttcaggact
     6421 ttgccatagc gtgatcgcgt tcccctctcc cagcgggagc gggcgaaaaa ccgcgccccg
     6481 tgctgtcctg aacacgatga tgagctttgc caaccgggtc gagtggaaga gccctcgacc
     6541 tgccgcacgg tttcattacg ggctgaagat tttcgcgcgg tttttgcggt ccgtcgtcgg
     6601 cggaaccttt ttccagcggt agccgagcgt cgcgttgtcc gtaacctgcg gcagcaccga
     6661 gagccggagc cgctggcagc agagcggcgt gaagcgcacc gtttcgagcg gctcgctcga
     6721 atacgcgggc gacaactgga gccgtgccgg ttcgttgtct tgcagcgtcc actcggggat
     6781 ccgtcggcag cggaccgaca gctcgcagca ggcgttggac gagacgaagc agtcgtcgtt
     6841 ccacacgcac tccttccaga ccggcttcgc ggtaacatcg atgccgtagt tccaaggcgt
     6901 ggtcggcgcg atctcggtgt agacgcggtc ggcatcacgg ttgttgtttc cctccaccca
     6961 gatgacggaa ccgctatcgc ccttcaccac cgacaccttg gaataccgct cgccgatcgc
     7021 caccgaatag gtgagcgggc cgcggtcgac cgaaatgccg ttctgtcgtg cccacggtgt
     7081 gaactcgcac tccgccttca tcgcgatgtc gagcgtagat ccgccggcgg gcaccttcac
     7141 gtccagcatt ctcacgccgg gtgtcgcggc ggtcccgccg acggagaacg cctttgccca
     7201 agcgggtacg cggaagcgca ggacaagcgg tttcgccgcg ttcaccgcca gccgggcggt
     7261 ttcgcggaac ggatagcgtg tatcgagttt ccattcgaca ccgtccgttt tccccgagca
     7321 cggcgcgtac atccagaaga cgagtgcgcc gtccctcgcg cgcttcacca gattctcggt
     7381 gaagagcggg aacggcattg ccgcgttgta gaggcaacag cggtactcag tgtgcgaata
     7441 ggcattcatc ggcggatggt tcgcatagtc gtggtcggtg cgcgcatcaa tcaccgtctg
     7501 gttgcaggcg gtgatgtagt ggacctcgtt ccaatccggc gtgtaggcac atggagcgtg
     7561 gttgaagacc acatcctcgg tgcggtctgc ccacttcgtc tcggcgcggt ggtcggagag
     7621 gttctggaag ctccgcacgt attcacccca cgagcagctc tccagtccgt aacgcggatc
     7681 gatacagccg acgcggcagt tctcatccgc cgcgaacgcg caacgcggca tctgtcccca
     7741 ggtcatccag tgttgcttca gccagtactc gctctcctcg gcaagcgcga gatcgccggt
     7801 gcgccgcgcg tagatcgaag gataggagaa catctggcag aaattaacgt tgtggatgtt
     7861 gaggaagagc gattcgcgct taacgtagta acgccgccac accttgtccg aaagccgcgt
     7921 gaacttcgca tccttcgtca cgtcataaag ccggaacagc gttgagatca tatcgccggc
     7981 gcgcgctttc tggatgaggg ggagccatcc cgaggccggc gcgtaaccgt tgccctgcgg
     8041 cgggtggatg aagcggtcct cgggaacggc aagacagaaa tcgcagaagc cggaaagcag
     8101 ggccatcacg cgccggtcgc cggagtagtc ataccacgaa aggagcgcct cgttcatcac
     8161 catgtggccc cagatgtcct gcatgaaagt cccgtcctta agccgtaccc gcttgagcgg
     8221 ctggggaccg taccagccgt ccggatcgcg cgtcgcgagc atcttctccg tccagtcgcg
     8281 gcagacgccc aacatccgtt cgttaccggt cagcgcggcg agcttcacga acgaacggaa
     8341 ccaatagggc tgttcctccc atccggtgcc ctcgttccag tggcgctggt tgtcgagttc
     8401 gccctgctcg gcgcgcgaga tccagccgtt ggtggggttc aggaacttgc cgtattcgta
     8461 aagtcgccca cagatgccgt ccgtcatttt gtcgagctgg tgtttgagcc atccctccgg
     8521 gcagattgac ccgagcggca gtcgcttcag ctcacagtcc tgcaattcgt ccgccgcgac
     8581 cttcgaggca cacgctatcg ccagtagcaa cacagccacg gcggtcgcaa tgggcgtatc
     8641 gttcatcttt gtcatgtgcg tttctccttg ggggcgaagg aggcgtgttt gcacattggg
     8701 ttgcgctgaa aacgtcgcca ccgcccctcg ttctcaaaaa ccccggcgaa gtaatgcgga
     8761 cggagcccgc acccctttgc ttacaggtgc cgaatctgcg gtatcgtttt cacgatcacc
     8821 ggaggatgaa tgtcgttccc gacttgctga ccgtcatgcg gatcgcgcct tcgaccaccc
     8881 tgaaggagac gttgcgcttt tcgggatgga caacctgggt gagattgcgg ccccatccgc
     8941 tcagatcggt cgacgcatgg atcgaggcag acttgaagac atcggaatct ccaaaggcga
     9001 ggccggtgaa atcgccgacg accttgatcg tgccgttcac cgggaggacg agttcacctt
     9061 cgatcgtctg agaggacgcc accccgtcgg tgatcaccac cgtcagctcc gcattgttct
     9121 gcagtgtcac gtcacccgtg accgtgccac cgccgccgag acccgttgcc gtgagcggct
     9181 cgccgcccac cactttcagc atcgcgttcg tccccagcat caccttgcct gccacggccg
     9241 cctcgtaacc ggccgttgaa tagccctccc ggcaccactt attacgaagg tacgtttcga
     9301 tgcggtgcgc ctcgggacgc gacaccggcc gtgtgtagaa catcacctcg ccatactgcg
     9361 cgccaccgaa cttgttgttg tagtggatac agttgaaagc cgaggcgctc accggatcga
     9421 tgtaggtcct gagcgagatg acattgagat cgttgtcgag ccccgttgtc gtcggatcgg
     9481 tttcaacacc gtttaccttc acgtatgtac agtacccctt ggcgagcgaa taggaattcg
     9541 cataccagcc attgggatag atcagcggcg ccgccggtcc cagtgcggcc gcgttgtcac
     9601 cgcggtagag gccgccctcc ccataatttt gataaatg
//
LOCUS       DAOSRB010000108         9564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6906_length_9564_cov_7.697970, whole genome shotgun sequence.
ACCESSION   DAOSRB010000108 DAOSRB010000000
VERSION     DAOSRB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6906_length_9564_cov_7.697970"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1129)
                     /locus_tag="PLZ74_07070"
     CDS             complement(122..1129)
                     /locus_tag="PLZ74_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007468506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOR98119.1"
                     /translation="MGRFTVAEFCVGGGGQALGLEAAGFDCVAAVEVDADCCTTLRLN
                     RPQWNVLHEDMRQVDGSRWRDTDLFAAGVPCPPFSIAGKQLGTEDERDLFPDAIRIIR
                     EMKPKAVMLENVPGFASAKFSEYRDSLRIQFLDMGYESDWRILQAADYGVPQLRPRFV
                     LVALPSDRMSFFEWPQPLSEQTTVGDTLQDLMAQRGWKGAQKWVKNARKIAPTIVGGS
                     KKHGGPDLGPTRARKQWRELGVDGLGIANEPPGADFPKEAFPRLTVRMVARIQGFPDF
                     WKFSGRKTAAYRQVGNAFPPPVAASVGLAIANAFDGKYPVRQPRKAIQLKLLEQTVPY
                     C"
     gene            1332..1487
                     /locus_tag="PLZ74_07075"
     CDS             1332..1487
                     /locus_tag="PLZ74_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98120.1"
                     /translation="MQKRTVKTGTEPQWRVPATERATLNASASNLLDDDFQLYPGQEP
                     APSLTYA"
     gene            complement(1539..3404)
                     /locus_tag="PLZ74_07080"
     CDS             complement(1539..3404)
                     /locus_tag="PLZ74_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98121.1"
                     /translation="MSVLLAAALAMTLEPRTYAPEEIKVEKGGTPDAPLVIKAAERGK
                     SVISGAIAPKGWQTWRGEILVADLPLDPGAGELCLYQDGERMDNARWPNEGYAQIDDC
                     LGENTRATHDGGGKAWLDGIAVFDSPRLAAWAKEPEPKVFGLWFYLWAEQRSRLLAVD
                     PQKKTFTVEMIGEAKKWGMRPKANFFVYNMLSEIDRPGEWAIERKAGKVYFWPKKDVQ
                     KFPPVFAGGKTLVLKNLGNVVFDGLVFEYFTDSAVALDRTDNVEIRSCLFRHTGGWAI
                     WSANASKTRVVGCDMYDLGEGGVLLDGGDRMKLEKGDNVVDNCHIHDYGKLRPNYRPA
                     VQLNGCGNRASHNLIYNGDHQALAFRGNDHLLEYNVIHDMCRFNYDAGCIYACTRDWS
                     HRGTVIRHNLIYMTGMRPQTSATDAIYLDDWTSGVTCYGNIINRCTRGIHMGGGQDNH
                     FYRNLIINADLSVCLYSRGADGGFSAKTANMGKDSFIYKRLTDHLDVYGSEKWKAAYP
                     KMLDLLKHDPVDAHLQFDNVVTGNVFVACGSMLLPGAKARETCVTEPNETRTGDPGFA
                     DYGNFDWRIDGFEFEKMGLYDDPRRFSPAMKFGPNVYRPAPPFRGSRLDGKENSF"
     gene            complement(3452..5197)
                     /locus_tag="PLZ74_07085"
     CDS             complement(3452..5197)
                     /locus_tag="PLZ74_07085"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HOR98122.1"
                     /translation="MMIRTMTVCAAALGLSAAAVGEQVDNRVRTLIHPVRVVATFPKA
                     GENYGDPAKVIAPEALLAGAPQQKNCGGVWKTSGCQLVNSGVAPGIVLDFGREIHGGL
                     QIMNCSQHGMKVRVRFGESVGETLVDIGDRGASNDHAIRDGVYSVPHMGAMEIGNTGF
                     RFVRIDLVTAGKLALDCVRAVSLMRPMKRLGAFKSSDERLNRIWETAVQTVHLCCQDQ
                     LWDGIKRDRLVWMGDTHPESMAILNVFGADAVLPETLDYAMHITDPDEKWMMNMAPYT
                     LWWLRNVYEWYFFTGDAEYVHQRGDYIRRTVRHCLSTMKDGTWECPRRVFLDWPSQHD
                     LVASKEGLQGLAAITFENSAEFAKILGDAALEADCRAAVAALRKRGGDARGCKQAAAM
                     LALSDLAEPKRMFDEVLGKNGHEGVSTFYGYYMLEAMSKSGANQRALDTIRDFWGAML
                     DVGATSFWENFSLAWTNNCFRIDELPIAGKKDIHGDYGEFCYPGFRHSLCHGWSCGPA
                     SWCIQHVLGIRVLEPGCRSIEIKPFLGDLDWAEGAMALPDGGCIKVRVEKRTDGRLKT
                     DVECDMEHVKWRVRD"
     gene            complement(5222..6868)
                     /locus_tag="PLZ74_07090"
     CDS             complement(5222..6868)
                     /locus_tag="PLZ74_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98123.1"
                     /translation="MFADALLLATLQIFSQVPVEDFNAANQARYLKDIRDAGITTVLV
                     SYSDYYGIGDSRKPYFAKIGREVRYFEEAGLDVRVWCNGFGYGNDRRVGAGKTRFANS
                     QNIVSMHGKTSGALCPLDPAMRRLLIENMRDIAATGVKAILMDDDFIQSARGFVGCAC
                     DRHLARVAKKVGHPVTHADMVRAFTGKPNALRTAFVDVTGEVAVELARELRAAVDEVN
                     PQTRIGLCLSVTHWDLEGVDMDELLRAFAGETRPFVRISGAPYWDDARFRGIGLEGVT
                     EFARMQAQWLQGKGYEIFDENDPYPRKVKVVPAWRCELYDKATIADGLMARHKYMLCY
                     GKDRTEPGYIEAHLANMKDDRKLAELFEGAEPYGLRIDFPKRRIREATLPAEPLEERR
                     ITELYSQPLAANLLLRCGIPIRHDDATASYVFRPEDFGIDVAAVDFAAFKGGDLRKRI
                     LEDVRRKSGRPLDVYVETASPRIYQIVRYNAKRSEYAVLLENMGTKSAEVKIVTAGTP
                     RITGSVCGKFATGPGGVTLADLPAHEFAAVRFALNDETGK"
     gene            complement(6858..9251)
                     /locus_tag="PLZ74_07095"
     CDS             complement(6858..9251)
                     /locus_tag="PLZ74_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOR98124.1"
                     /translation="MRRQATLLWTALSVGLFMDAAAAENGLVAHFTFDNPTQFGYDNV
                     QQAVIGTITSDSISGATPADPTSCDAPIVRGMQIATGWWQNNYMTVPGASFGSAQGIP
                     YGNQAVTYSLWIKPSPNWQTSGTLGMGTGCYLLRHCTAGHEWYKTYDHECLYIIRDGS
                     GFPTITFSVGEYNAAAASATYALGAAFDGGWHLITATYENRVLKLYWDGVKRAETTIA
                     ANVGVPNNSPLMFGHAAVDYNTYVQRRFAGAVDDIKVYNRALDADEILAAYNAGATAF
                     DTDVIVWKGASAGGAAEDGDNWSTKSNRRTVGEVYTAGAVFDVTAVTDDGTITHNADT
                     TLKLKGVICTNGQDGVTIRMQAGDIEMKRPSTQRGLVAHFAFDDPDNMYCDLSTNAFT
                     ATPGAGVAYGSANPVPLSGCAGITGNGLNFVNGWWLFDYLTVSGSCFNAAHGIPYGNQ
                     AVSYSFWIKPTPTWRDYQHLGLGHNGVYLLRHGENLCQWMDNRGHSFWIFKDGDNPKI
                     TWSAANGGAAAARTAAYTSTELFDGAWHQIAGTYEDKVLKLYFDGELKATTTVPDNLT
                     VANNSSLIVGADGLTNMDHSVQRTYAGGFDELKVFNVALTDAEVAAEYAQRSRNLDKV
                     PAGTVPSPAKVQLDAGTSVAVRGYGHQYGSLTGAGSVDVGYTSALTLDGTFAVAGALT
                     GGGEISAANLVLGGNASGFTGDFTLGENATVTITDSSDTFLNSTFGGKVTLPKKAVIA
                     YGGKRPNGGMATLTAATLGTPSDFTEWVDSTGGTVKVRAVDDTLQIQVIGGLVLYVR"
BASE COUNT         1818 a         3124 c         2701 g         1921 t
ORIGIN      
        1 ccgctccatc cgccgcttcc tgaagctcat gcgattcgat aacgcgaccg atgtttgcta
       61 acaagaaccg tctggttttc tcttttgaac cgactttgcc catgttcccc tcatttttat
      121 ctcaacaata tggaactgtc tgctccaaca gtttcagctg aatggctttg cgtggctgtc
      181 gaaccggata tttgccatca aaagcattgg caatcgccaa tccgacagat gcggcgacgg
      241 ggggcgggaa ggcattgccg acttgacggt atgccgcagt ctttcgcccg ctgaacttcc
      301 aaaaatcagg aaacccttgt attctcgcaa ccatgcgaac agtcaggcgg gggaaggcct
      361 ctttcggaaa atcggcgccg gggggctcgt tggctatgcc caggccatca acacccaatt
      421 cgcgccactg tttccgggcg cgcgtgggtc ccaagtcggg gccgccgtgc ttctttgagc
      481 cgccgacgat ggtgggcgcg atcttccgtg cgttctttac ccatttttga gcgcctttcc
      541 atcccctttg cgccatcaag tcctgaagcg tatcgccaac ggttgtttgc tcgctgagcg
      601 gttgcggcca ctcaaaaaaa gacattctgt ccgagggaag cgcgacaaga acaaatcgag
      661 gacgcagttg aggcaccccg tagtcggctg cctgtagaat ccgccaatcg gactcatagc
      721 ccatgtccag aaactgtatg cgcaagctgt cgcgatactc agagaatttg gccgaggcga
      781 atccggggac attttccaac atgaccgcct tgggcttcat ctctcggatg attctgattg
      841 catctgggaa caagtcccgc tcgtcttcgg tgccgagttg tttgccggca atggaaaagg
      901 gggggcaggg aacgccggcg gcaaacaaat cggtgtcgcg ccatcgcgag ccgtcaacct
      961 gtcgcatgtc ttcgtgaagc acgttccact gcgggcgatt gaggcgcagc gtcgtgcagc
     1021 agtccgcatc aacctcgact gcggcaacac agtcgaaccc agcggcctca agcccaagag
     1081 cctgaccgcc gccgccaacg caaaactctg ccactgtgaa cctgcccatg gtgccggtac
     1141 ttataccatc cttttcgccg gaaacgcaag tcccgcgccg taatcggtcg gcaacgaagg
     1201 gacaggtgtt tagacggctt tcgtgtgtaa aaccggttcc gatgcggata ttgaacgtcc
     1261 cgctatatgt aatggatggg tcttgtccta tttcctgttg ggggcggagc aaaaacggac
     1321 acaattgcaa aatgcaaaag cggactgtca agaccggcac cgaaccgcag tggcgcgtgc
     1381 ccgccaccga gcgcgccacc ctcaacgcga gtgccagcaa tctgctggac gacgacttcc
     1441 agctctaccc cggccaagaa cccgccccct ctcttactta tgcgtgatgg gagcgtggct
     1501 ggttgaacgc tggaagttga ctgtggaatg ttacgcactc agaaggagtt ctccttgcca
     1561 tcaagccgcg aaccgcggaa cggcggagcg gggcggtaga cgtttgggcc gaacttcatc
     1621 gccggcgaga accgccgcgg atcgtcgtac agccccatct tctcgaattc gaacccgtca
     1681 atgcgccagt cgaagttccc atagtccgcg aatccgggat cacccgtacg cgtctcgttc
     1741 ggttcggtga cacaggtttc acgtgccttc gcgccgggca ggagcatgga gccgcaggcg
     1801 acgaacacgt tgccggtcac gacgttgtcg aattgaagat gcgcatcgac cggatcatgc
     1861 ttgagaagat cgagcatctt cggatacgcc gccttccact tttccgaacc atagacgtca
     1921 agatgatctg tgaggcgttt gtagatgaaa ctgtccttcc ccatgttcgc ggtcttggct
     1981 gagaaaccgc cgtctgcccc gcggctgtag aggcagacgg aaagatccgc attgatgatg
     2041 agattgcggt agaaatggtt gtcctgtccg ccgcccatat ggatgccgcg cgtgcagcgg
     2101 ttgatgatgt tgccatagca cgtgacgccg ctcgtccagt catcgaggta gatcgcgtcc
     2161 gtggcggagg tctgcggacg catcccggtc atgtagatga gattgtgccg gatcaccgtg
     2221 ccgcggtgcg accagtcgcg cgtgcaggcg tagatgcaac cggcatcgta attgaatctg
     2281 cacatgtcgt ggatgacgtt gtattcgagc aggtggtcgt taccccggaa ggcgagcgcc
     2341 tgatggtcac cgttgtagat gagattgtgt gaggcgcggt tgccgcagcc attgagctgc
     2401 accgccggac ggtagttcgg cctcaacttt ccgtagtcgt ggatgtggca gttgtcgacg
     2461 acgttgtccc ccttttcaag tttcatccgg tctccgccat cgagcaggac gccgccctcg
     2521 ccgagatcgt acatatcgca gccgacgacg cgtgtctttg acgcgtttgc gctccaaatc
     2581 gcccagcctc ccgtgtggcg gaacagacac gagcggattt cgacattgtc cgtacgatcc
     2641 aacgccaccg cgctgtcagt gaaatactcg aagacgagcc catcgaagac gacgttcccc
     2701 agattcttca aaaccagcgt cttcccgccg gcgaaaacgg gcgggaactt ctggacatcc
     2761 ttcttcggcc agaaatacac cttccccgcc ttccgctcga tggcccactc gccgggccgg
     2821 tcgatctcgg agagcatgtt gtagacgaag aagttagcct tcggacgcat tccccacttc
     2881 tttgcctcgc cgatcatctc aactgtaaag gtcttcttct gcggatcgac cgcgagcagc
     2941 cgcgaacgct gctctgccca gagatagaac cagagtccga acaccttcgg ttccggctcc
     3001 ttcgcccagg cggcgagacg gggcgaatcg aagacggcga tgccgtccag ccacgccttg
     3061 ccgccgccgt cgtgcgtcgc ccgcgtgttt tctcccagac agtcgtcgat ctgcgcgtac
     3121 ccctcgttcg gccaacgcgc attatccatc cgctcgccgt cttgatagag gcaaagctcg
     3181 cccgcccccg gatccagcgg cagatccgca accagaatct caccgcgcca cgtctgccag
     3241 cccttcggcg cgatcgcgcc cgaaatcaca gactttccgc gctccgccgc cttgatcacg
     3301 agcggagcat ccggcgtccc tcccttttcg accttgattt cctcaggcgc atacgtcctt
     3361 ggctccaacg tcatcgccag cgccgccgca agtagaacac tcataaaaca tgcctcccct
     3421 ggtttccttg ccttttccta aatcccggtt cctaatccct cacccgccat ttcacatgtt
     3481 ccatgtcaca ctccacatcc gtcttgagcc taccatccgt acgcttctcc acgcgcacct
     3541 tgatgcatcc gccatccggg agcgccatcg cgccctccgc ccagtcgaga tcaccgagga
     3601 acggcttgat ctcgatcgac cggcagccgg gctccagcac ccggatgccg agcacatgtt
     3661 gaatgcacca cgaagcgggt ccgcagctcc agccgtgaca gagcgaatga cggaacccgg
     3721 gatagcagaa ctcgccgtag tcgccgtgga tgtccttctt cccggcgatt ggaagctcgt
     3781 cgatgcggaa gcagttgttg gtccaggcca gactgaaatt ctcccagaag ctcgtcgctc
     3841 ccacatcgag catcgcgccc cagaagtcgc gtatcgtgtc gagtgcgcgc tgattcgctc
     3901 cggacttgct cattgcctcg agcatgtagt agccgtagaa cgtggagacg ccctcgtgac
     3961 cgttctttcc gagaacttcg tcgaacatcc gcttcggctc cgcaaggtcc gacagcgcca
     4021 gcatcgccgc cgcctgtttg catccgcgcg catcgccacc gcgtttccgg agcgccgcga
     4081 ccgccgcgcg gcaatccgcc tcgagcgccg cgtcgccgag aatcttcgca aactctgcgc
     4141 tattctcgaa ggtgatcgcc gccagcccct ggagcccctc cttcgacgcg acgagatcat
     4201 gctgcgacgg ccagtcgagg aagacacgcc tcggacactc ccaggttccg tccttcatcg
     4261 tcgagaggca gtgtcgcacc gttctgcgga tatagtcgcc gcgttggtgg acatattccg
     4321 cgtcgccggt gaagaagtac cactcgtaga cattccgcag ccaccagagc gtgtacggcg
     4381 ccatgttcat catccatttc tcgtccggat cggtgatgtg cattgcgtag tcgagtgtct
     4441 caggcagaac cgcgtccgcg ccgaagacgt tgaggatcgc catcgactct gggtgcgtgt
     4501 cgcccatcca gacaagccgg tcccgcttga tgccgtccca taactgatcc tggcaacaga
     4561 gatgcaccgt ctgcacggcg gtctcccata ttcggttcag gcgttcgtcg gacgacttga
     4621 acgcgcccag ccgcttcatc ggccgcatca gcgataccgc ccggacgcag tccagcgcca
     4681 acttcccggc tgtcaccaga tcgattcgca cgaaccggaa cccggtgttg ccgatctcca
     4741 tcgcgcccat gtgcggcaca gaatagacac cgtcacggat cgcatggtcg tttgacgcgc
     4801 ccctgtcccc gatgtccacc agcgtctcgc cgaccgattc gccgaaacgg acgcgcacct
     4861 tcatgccgtg ctggctgcag ttcatgatct ggagcccgcc gtggatctcg cgtccgaagt
     4921 cgagcacgat tcccggcgcg acgccgctgt tcacgagttg acagcccgag gtcttccaga
     4981 cgccgccgca attcttctgt tgcggcgccc cggcgagaag cgcctccggc gcgatcacct
     5041 tcgcgggatc gccataattc tcgcccgcct tggggaacgt cgccaccacc cgtacgggat
     5101 gtatgagcgt ccgcacgcgg ttgtccacct gctccccgac cgcagccgca ctcaacccga
     5161 gcgccgcagc acagaccgtc attgtcctga tcatcatcgc gcttttcctt cggttcccat
     5221 cctattttcc ggtctcgtca ttcaacgcga aacgcacggc ggcgaattca tgggcgggaa
     5281 gatccgcaag cgtgacacca ccgggaccgg ttgcaaattt tccgcagacg ctgccggtga
     5341 ttcggggcgt acccgccgtc acgatcttca cctcggcaga cttcgtcccc atattctcca
     5401 gcaacacggc gtactcgctg cgctttgcgt tgtaccgcac aatctggtag atgcgcggtg
     5461 aagcggtctc gacgtacacg tcgagcgggc gtcccgactt cctccggaca tcctcgagaa
     5521 tccgcttccg cagatcgccg cccttgaacg cggcgaaatc aaccgccgcc acgtcgatgc
     5581 cgaaatcctc cggacggaag acgtacgaag cggttgcgtc atcgtgccgt atcgggatgc
     5641 cgcaccgcag cagaaggttc gccgccagcg gctgggaata gagctcggtg atgcgccgtt
     5701 cttcaagcgg ctccgccgga agagtcgcct cgcggatacg ccgcttcggg aaatcgatac
     5761 ggagaccgta gggttcggcc ccctcgaaca gttctgccag tttccggtcg tccttcatat
     5821 tcgcgagatg cgcctcgatg taccccggct ctgttcgatc cttcccgtag cagagcatgt
     5881 acttgtgccg cgccatgagg ccgtccgcga tcgtcgcctt gtcgtagagt tcgcagcgcc
     5941 acgcaggaac caccttgacc ttgcgtggat acgggtcgtt ctcgtcgaag atctcgtatc
     6001 ccttcccttg cagccactgt gcctgcatcc gcgcgaactc cgtcacgccc tcgagcccga
     6061 tgccgcggaa tctcgcgtcg tcccagtacg gcgccccgct gatgcggacg aacggacgcg
     6121 tctcccctgc gaacgcccgc agcagttcgt ccatgtcgac gccctcgaga tcccagtggg
     6181 tcacgctgag gcagagcccg atgcgcgttt ggggattcac ctcgtcaacc gccgcgcgca
     6241 gttcccgcgc aagttccacc gcgacctcgc ccgtcacgtc gacaaacgcc gtgcgcagcg
     6301 cgttgggctt ccctgtgaac gcccggacca tatccgcatg cgtcaccggg tggccgacct
     6361 tcttcgccac ccgcgcgaga tgacggtcgc aggcacagcc gacgaagccg cgggccgact
     6421 gaatgaagtc gtcgtccatc aggatcgcct tcacccccgt cgcggcgata tcgcgcatgt
     6481 tctcgatgag cagccgacgc atcgcgggat cgagcggaca gagcgcaccg ctcgtcttcc
     6541 cgtgcattga gacgatgttc tgcgagttcg cgaaccgtgt cttccccgcc ccgacccgac
     6601 gatcattgcc gtagccgaag ccgttgcacc agacgcgcac atcgagcccc gcctcctcga
     6661 aataccggac ctcccgcccg atcttcgcaa aatacggttt gcgactgtcc ccgatgccat
     6721 agtagtcgct atacgagacg agtaccgtgg tgatcccggc atcgcggata tccttcaggt
     6781 acctcgcttg attcgccgcg ttgaaatctt cgaccggcac ctgcgagaag atctgcagcg
     6841 tcgccagcag tagcgcatca gcgaacatag agcaccaagc cgccgatgac ctggatctga
     6901 agcgtatcgt ccacggcccg caccttgacg gtaccgccag tcgaatcaac ccactcggtg
     6961 aagtcgctcg gcgttcccaa cgtcgccgcg gtaagggttg ccatcccgcc attcggacgc
     7021 ttcccgccat aggcgatcac cgccttcttc ggcagcgtca ccttgccgcc gaaggtcgaa
     7081 ttgaggaacg tgtcggaact atcggtaatc gtgaccgttg cattctcgcc gagcgtgaaa
     7141 tcgccggtaa atcccgatgc gtttccgccg agcacaagat tcgccgccga gatctccccg
     7201 ccgccggtga gcgcgccggc gaccgcaaac gtgccgtcga gtgtgagcgc ggacgtgtag
     7261 ccgacgtcca ccgaacccgc gccggtcagg gaaccgtact ggtgcccgta cccgcgcacc
     7321 gcaaccgacg tgccggcgtc gagttgcacc ttggccggcg aaggcaccgt ccccgccggg
     7381 accttgtcga gattccggga acgctgcgca tactccgccg ccacctcggc gtccgtcagc
     7441 gcgacgttga agaccttgag ttcgtcaaaa ccgccggcgt aggtacgctg cacggagtga
     7501 tccatatttg tcaatccgtc ggcaccgacg atcaggctgc tgttgttggc gacggtgagg
     7561 ttgtcgggaa ccgtcgtcgt cgccttgagc tcaccgtcaa aataaagttt cagcaccttg
     7621 tcctcgtacg tgccggcgat ctgatgccac gctccgtcga acagttcggt cgaggtatag
     7681 gctgcggtcc gggccgctgc cgcaccgcca ttcgctgctg accaggtgat cttgggattg
     7741 tcgccgtcct tgaagatcca gaaggaatgt ccgcggttgt ccatccactg gcataggttt
     7801 tctccatggc gaagcagata caccccgttg tgaccgaggc cgagatgttg gtaatcgcgc
     7861 catgtcggag tgggcttgat ccagaacgaa taggagaccg cctgattccc atacgggatg
     7921 ccgtgcgcgg cattgaaaca cgaaccggaa accgtgagat agtcgaacag ccaccaaccg
     7981 ttcacgaaat tcaaaccatt gcccgtgatg ccggcacaac cgctcagcgg caccggattg
     8041 gcggatccgt aggcaacgcc ggcgccgggc gtcgccgtga aggcgtttgt ggaaagatcg
     8101 caatacatgt tgtccggatc gtcgaaggcg aagtgcgcca cgagtccgcg ctgggtcgag
     8161 gggcgcttca tctcgatgtc gcccgcctgc atccggatcg tcaccccgtc ctgtccgttc
     8221 gtgcagatca ctcccttgag cttcagcgtc gtgtcggcgt tgtgggtgat cgttccgtca
     8281 tccgtgaccg ccgtcacgtc gaagaccgca ccggcggtgt agacctcacc gaccgtacgg
     8341 cggttggact tcgtcgacca gttgtcgccg tcctctgccg cgccaccggc cgatgcgccc
     8401 ttccagacga tcacgtcggt gtcgaacgcg gtcgcccccg cgttgtaggc ggcgagaatc
     8461 tcgtccgcgt cgagcgcgcg gttatagacc ttgatgtcgt ccacggcccc ggcgaagcgg
     8521 cgttgcacgt acgtgttgta atcaacagcg gcatggccga acatcagcgg cgaattgttc
     8581 gggacaccga cgttggcggc gatcgtcgtc tcggcccgct tcacgccgtc ccagtagagc
     8641 ttcaacacgc ggttttcgta ggtggcggtt atcaagtgcc agccaccgtc gaaggcggcc
     8701 cccagcgcat aggtggcgga ggccgccgcc gcgttgtatt cgccgaccga aaaagtgatc
     8761 gtcggaaatc ctgaaccgtc cctgatgatg tagagacact cgtggtcgta ggtcttgtac
     8821 cattcatgcc ccgccgtaca gtgccgcagc agatagcagc cggtacccat gccgagagta
     8881 ccactcgtct gccagttggg ggacggcttg atccacagcg aataggtgac cgcctgattc
     8941 ccatacggga tgccctgcgc ggatccgaac gacgcgcccg gcacggtcat gtagttgttc
     9001 tgccaccagc ccgtcgcgat ctgcatgccg cggacgatcg gcgcgtcgca ggatgtcgga
     9061 tctgcgggcg tggcaccaga gatgctgtca ctcgtgatcg tgccgatcac cgcctgctgc
     9121 acattgtcat acccgaactg cgtcgggttg tcgaaggtga agtgcgcgac aagcccgttc
     9181 tcggccgccg ccgcatccat aaacaaaccc accgacagcg ctgtccacag caacgttgct
     9241 tgacgtctca tcggaacact ccttttcatt gtaagtcgga ataaaacttt ccattatcat
     9301 accatcccat agggcagacc ggcaaccccc ggctcccacg gttagtacgc attatgcggg
     9361 gggggtcaac cctaaaactt gtaatcggtt cgtgacaggg gcatgcgaag gtcatgcgtt
     9421 tcagggttcc attgcggcga taccgatacc gatagtgatt tggatggaac catgaaaact
     9481 gtgcccccgc gcctctgtgc gaaaaaaccc ctaactcctt gcctataaat tgacaggatt
     9541 acaggatttt caggattgaa cagc
//
LOCUS       DAOSRB010000109         9560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6911_length_9560_cov_7.782325, whole genome shotgun sequence.
ACCESSION   DAOSRB010000109 DAOSRB010000000
VERSION     DAOSRB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6911_length_9560_cov_7.782325"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLZ74_07100"
     CDS             <1..383
                     /locus_tag="PLZ74_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOR98125.1"
                     /translation="SLDGLVTPIFMCFFTLSGADLDFGVFATVGFLAIVYVVTRVIGK
                     VAGATAGAKLANMPKTVQKYLGLTLVPQAGVAIGLSLAASRILGDSHGHEIRTIILAA
                     TVLYELSGPIITKIALTKAGEIEA"
     gene            complement(661..3021)
                     /locus_tag="PLZ74_07105"
     CDS             complement(661..3021)
                     /locus_tag="PLZ74_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98126.1"
                     /translation="MKRTSPSTIILALLIVATSSLCAREHDLAAIIKTQAERSSVWTR
                     VQDLTPTMTSRELFAYALLLCEAQLHLDRLDRLFEVAARMQDRNPESRGYGNFRWSWR
                     DGAVFDFNAGDFCMENAAVIWIKHRKLLTDKQRKDLSELIDHAIAGCLRHRVSSGYTN
                     IAILNAQNLILMGEMLGRDDVLAEGRKRLDDFCAYTAVNGIREYCSPTYAGVDLICLH
                     RLHTYCVTPAVRGQCEALLNVFWLDAIANCFAPSFRLGGAHSRDYDYLGGRGGVDAWL
                     AAAGCFASPNPSAAPPLLWLQSTWRPPTAWLRDLQVAPRVVTQVWGPGGSEYRVMWAG
                     RHVALSIAGKTYHNMDAPMVVNFAGPHTMPRGYFIADGRRDPYGKKVIPEGNGPHRKT
                     LHLMPHWYAVQDGQDALGLALHRREDIRDCPTLESHFVLPYPCDELFIGNHTVVFDAK
                     APKTVPVSSGAAVVIRHGAAAAAIRVIWSQNTVREPAPVALIHDGNPHGVIRLTVAHH
                     DQWGMPINTDAPVGAAFWIRVCDLADNAVHFARWRQEFAAATADARCDNASLAITAGS
                     TSGQTLRLLTHKPFRALSDAEPVPARAVLAVNGRDLGGEHLARLPVVVAYKRQIAEAL
                     TRQRKGAVMILPDRDAIWEAEAGAVFPNMVVAKDDPQASGGSYVWAPGEPGARGGGTG
                     YLLQNLQVAKAGQYYLSARVIAPSFEDDSFFISVSSEKFSILDQFVWHLPKVNAEWYW
                     AMVADRGNSDGIPLQLPEGTVTLRIQVREDGTKIDQLRLSPQPGVR"
     gene            3234..4739
                     /gene="zwf"
                     /locus_tag="PLZ74_07110"
     CDS             3234..4739
                     /gene="zwf"
                     /locus_tag="PLZ74_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOR98127.1"
                     /translation="MPKKRSAGTFCIEVAPSPCVLVLFGATGDLARRKLFPSLFQLHR
                     LGLLHAATRIVACGRSPVTDEAFREQSAAFLPQADPQTRTAFMSRLTYRQADAAEPST
                     FRDLATYLDGLDHAPESPVPLNRLYYLAVPPSSYRALITLLDNTGQINEHDHDQGPWR
                     HLVLEKPFGSDTATAADLDAFLKTVAKEEQLFRIDHYLGKETIQNIMVTRFANIIFDP
                     IWDARYVDHVQITAAETLGVEGRAAYFDHTGLLRDMFQNHIIEMLALTAMEPPERFDA
                     DAIHDSKLKLIRAIRPFDTGSDANAIVRAQYSAGRGMPAYREEAGIPTQSETETFVAA
                     KLWIDTPRWAGTPFYLRSGKRLAANSNAITLVFKQAPHLDLGGPGANALILRSRPGEG
                     MCLHLLGKHPGPKLCVGTLPLSFDYAELAPGNEAPDAYARLLLDAMLHDHTLFVRSET
                     IRTAWELFTPVLDLWRDCPDRMPLHTYEAGSAGPAAAADLMAADGREWSSL"
     gene            complement(4954..6852)
                     /locus_tag="PLZ74_07115"
     CDS             complement(4954..6852)
                     /locus_tag="PLZ74_07115"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR98128.1"
                     /translation="MDTDRTENQAPRIFELPVHTFHTRLLRLLVRIVKRPVESITGLR
                     ACDKIYARTWRMPKDIPFCDRALQAIGIRTDFSEADLARIPKEGAVVVVANHPFGGIE
                     GIILLSLLRRVRPDVKAMANYLLGAIPEMQGDFILVDPFGSKNSAKANLRPLKESLSW
                     LKEGHLLIVFPSGEVSSFDRRTLRVRDPAWSASIAALTRKTNATVVPMFFPGRNNVFF
                     QIAGFLHPRFRTSLLPRQIANKTGWKITVRIGTPLSPKALEPFSLDDVQLIRYMRFRS
                     YLLAERETHRTRRFISLNPPMLPADPIIAPVPCETLTREIASLPESCRLIAAGDFAVY
                     IAAAHQIPSCLREIGRLRETTFRVVGEGTGNAIDLDAFDEYYNHLFMWNTARQEIVGS
                     YRLGLTDRILTERGIKGLYTRTLFKFDERLMTRIPPAIEMGRSFIRTEYQKAYTSLFL
                     LWKGISAFIGRNPHYRVLFGPVSITNEYREASRCMILASMRQTCMADELTWLVKPKFP
                     PKPPRRSEWCLKEYMEYLNDIDRVADYVAEIEPDGKDIPVLLRQYLKLSGKLLAFNVD
                     PDFSSVVDGLIMVDLSQSSPKTLRRYMGNEVAEAYFRYHGITESTDDPAGKREPHSTP
                     AASSPTPP"
     gene            complement(6842..8155)
                     /locus_tag="PLZ74_07120"
     CDS             complement(6842..8155)
                     /locus_tag="PLZ74_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOR98129.1"
                     /translation="MTRAYIRPLSRAAFPGFALAALCAAPTQWSLIPSLRIGVADLLL
                     ACAAALVVLECLLARDGKKALSLRPHWTHLLFILASGAALSAATDRMDALKEWVQGVE
                     YFIIAPVVFTALLRKERLFSNASAEGAASPAASRVPPGFIALGAVTAVVLGLAVIQYL
                     APEGHDSIRLFAEPKTLDIIVNVLGGRESPLLVGGTFGNRNVLGGFLALALPLCAVGA
                     LASGRAIWTRIACALLLLTGLAVNLSGASYWAVVVVIAAIAATRGARVFLPVMLALML
                     WQTEVLKNLPRENDLCHFESVALYDSAGLPTRRYPEWQAAVGMILTHPWLGVGPGHYQ
                     KRIGSFYGQVPNATGPAEPDIQNLYLVLGASCGLPALFAFLLMLASALGAATRSGRWG
                     AAGALAAFAVTAVWHPLLVRGIGLPLAFVLALAHHPKSPEADYGY"
     gene            complement(8152..8679)
                     /locus_tag="PLZ74_07125"
     CDS             complement(8152..8679)
                     /locus_tag="PLZ74_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98130.1"
                     /translation="MQRMILLAGLFLICRAQAKGVRICWEAETANRIEPPVTVVSNGI
                     EGASGAYLEIAEGAGNPPKVNAGVAGFDLDIPEDGVFTLWCRVWWEGECSNSFTVRVD
                     DQPPFLFGEDATYKTWHWVKYPVSRTAPPLRLTRGRHTLTFLNREDGVRLDQILLSSD
                     RRFVPTDIEKTGVRQ"
     gene            complement(8766..8841)
                     /locus_tag="PLZ74_07130"
     tRNA            complement(8766..8841)
                     /locus_tag="PLZ74_07130"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8806..8808),aa:Phe,seq:gaa)
     gene            9172..>9560
                     /locus_tag="PLZ74_07135"
     CDS             9172..>9560
                     /locus_tag="PLZ74_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOR98131.1"
                     /translation="MQYRKLGSSEIQVSAVALGTWSIGGGPWWGKTDDNDSIRAIQAA
                     VDAGITLIDTAPAYGFGRSEEVVGRAIRGRRDQVVVATKCGLWWGDDRGVLHFEQDGY
                     TVRRCLDPRTIGVLNGSTACACVCMRS"
BASE COUNT         1870 a         2794 c         3114 g         1782 t
ORIGIN      
        1 cctcgctgga cggattggtc acccccatct tcatgtgctt ctttacgctc tcgggtgccg
       61 atcttgattt cggagtcttc gctacggtcg gctttctcgc gattgtctat gtcgtgaccc
      121 gcgtcatcgg caaggttgcc ggggcaacag ccggggccaa gctcgcgaat atgcccaaga
      181 ccgtccaaaa gtatctgggg cttacgctcg tgccgcaggc gggcgtggcg atcggcctgt
      241 cgcttgcggc atcacgcatc ttgggggatt ctcacgggca tgagatccgc acgattattc
      301 tcgcggcgac cgttctgtac gagctgtcgg ggccgatcat taccaagatc gcgctgacaa
      361 aagccgggga gatcgaggcc taatgatgcg ggcgaagccc gcaactcaag ttcattaggt
      421 cattagttaa ttagtgcatt agttaattag tgcattagtt aattagtgca ttagttaatt
      481 agtggattag tggattagtg gattagtttg tctgggagcg cgctgccgcg cttcgcagtg
      541 ctcctccaaa aaaccttggc gttcttggcg ccttggcggt tcccaaaagc cgatcaatgc
      601 cccccaaaaa aaccttcgtg cccttgtgtg agaaacaact tttttattgg ttgcaaaaag
      661 tcagcgcaca ccgggttggg gcgacagccg cagttgatcg attttggtgc cgtcctcgcg
      721 aacctgtatg cgcagggtca ccgtgccttc aggaagttgc agggggatgc cgtcggaatt
      781 gccgcggtca gcgaccatgg cccagtacca ctccgcgttc accttcggca aatgccagac
      841 aaactggtcc aggatagaaa acttctcact gctgacggag ataaagaacg agtcgtcttc
      901 aaacgaaggc gcgatgacac gcgcggagag gtagtactgc ccggctttgg cgacttggag
      961 gttttgcagg agatagccag tcccgccgcc gcgcgcaccg ggttcgccgg gagcccacac
     1021 atagctgccc cctgatgcct gcgggtcgtc cttggccacc accatgttcg gaaagaccgc
     1081 gccggcttcc gcttcccaga tggcatcgcg atcgggcagg atcatgacgg cgcctttccg
     1141 ttgtcgcgtg agtgcctcgg cgatctgccg tttgtacgcg acgacaaccg gtaagcgggc
     1201 caagtgttct ccgccgaggt cacggccatt gacggcaagg acggcacgcg cgggcacagg
     1261 ttcagcatca ctcagcgccc taaagggctt gtgcgtgagc aggcgtagcg tctgaccgct
     1321 ggtgctgccg gcggtgatcg cgaggctggc gttgtcacaa cgcgcgtcgg cggttgcggc
     1381 agcgaactcc tgccgccacc gcgcaaagtg cacggcgttg tcagccagat cgcagacgcg
     1441 aatccagaac gcggcgccga cgggggcgtc cgtgttaatg ggcatgcccc attgatcgtg
     1501 gtgggcgacg gtgagacgga tgacaccgtg ggggttgccg tcgtggatga gcgcgacggg
     1561 cgcgggttcg cgaaccgtat tctggctcca gatcacacgg atcgccgcag ccgccgcacc
     1621 gtggcggatg acgacggctg cgccggatga caccggcact gttttgggcg ctttggcgtc
     1681 gaagaccacg gtgtgattgc cgatgaacag ttcgtcacaa gggtaaggca acacaaagtg
     1741 ggattcgagg gtcgggcagt cccggatgtc ctcccgccgg tgcaatgcca gaccaagcgc
     1801 atcctgacca tcctgaaccg cataccagtg gggcatgagg tggagagttt tccgatgcgg
     1861 gccgttgccc tccggaatca cttttttccc gtagggatcg cgacggccgt cagcaatgaa
     1921 atagccgcgt ggcatggtgt gcggaccggc gaaattcacg accatcggcg catccatgtt
     1981 gtggtaggtc ttgccggcga tgctgagcgc gacgtgccgg ccggcccaca tgacgcggta
     2041 ttcgctaccg ccgggacccc acacctgcgt caccactcgc ggcgcgacct gcagatcgcg
     2101 aagccaagcc gtcggcggcc gccaagtgct ctgcagccac agcagcggcg gcgcggcact
     2161 cggattcggc gacgcgaaac agcccgccgc agccagccac gcatcgacac cgccgcgacc
     2221 gccgaggtag tcgtagtcgc ggctatgggc gccgcccagg cggaaggacg gcgcgaagca
     2281 gttggcgatg gcatcaagcc agaagacatt cagcaacgct tcgcattgcc cgcgcacggc
     2341 tggcgtgacg cagtacgtgt gcaggcggtg caggcagatc aggtcgacgc cggcataggt
     2401 cggactacag tattcacgga tgccgtttac cgccgtgtag gcgcagaagt cgtccagacg
     2461 cttgcggcct tcggcgagca cgtcgtcgcg tccgagcatc tcgcccatga gaatgaggtt
     2521 ctgggcgttc aggatggcga tgttggtgta accggaggac acgcggtgac gcagacaccc
     2581 cgcgatggcg tggtcgatca actcactcag gtccttgcgc tgtttgtcgg tgagcagctt
     2641 gcggtgtttg atccagatga ctgcggcatt ttccatacag aagtctccgg cattgaaatc
     2701 gaagacagcg ccgtcgcgcc agctccagcg gaagttcccg tagccacggc tttcggggtt
     2761 gcgatcctgc atgcgtgcgg cgacctcaaa gagccggtcc aagcggtcga ggtggagttg
     2821 cgcttcgcag agcagcagcg cataggcgaa gagttcacgg gaggtcatgg tcggcgtcag
     2881 gtcctgcacg cgggtccaca cgctcgaacg ttccgcctgg gttttgatga tggcggcgag
     2941 gtcatgctcc cgcgcacaca gcgacgaagt cgcgacgatg agtaatgcga gaataatggt
     3001 gctgggagat gttcgtttca tgggacctcc tataatgcct gcgacagtgt aaacatgccc
     3061 tttgagatta gggtaaaggt gtctctaagg caaggccaac gatgtgcgct ttggttttag
     3121 atttctggtt tcacgtttca tccgcagatg ggatttcctt ttttgggggg tcaccctagg
     3181 aggaaaacgc cttgcggatt gacttcgcgc cttaattgcg ttaccaatcg gacatgccaa
     3241 agaaaaggtc tgcgggaaca ttttgtatcg aggtcgcgcc cagcccttgc gtgctggtgc
     3301 ttttcggtgc caccggcgat cttgccagac gcaaactttt cccgtcgctg ttccagctgc
     3361 accgccttgg cctcctgcac gcggccaccc gcattgtcgc ttgcggacgt tcccctgtga
     3421 ccgacgaagc cttccgcgag caatccgccg ccttcctgcc gcaagccgac ccgcagaccc
     3481 gtaccgcgtt catgagccgt ctgacctacc gacaggcgga cgcggctgag ccctccactt
     3541 ttcgtgatct cgcgacgtat ctggatggac tggaccatgc gcccgaatcg cccgtgccgc
     3601 tgaaccgcct ctactatctg gccgtgccgc cttcctccta ccgggcgctc atcaccctgc
     3661 tcgacaatac cggccagatc aacgagcacg accacgacca aggcccgtgg cggcacctgg
     3721 tgttggaaaa gccgttcggc tcggataccg ctactgccgc cgatttggac gccttcctta
     3781 aaaccgtggc aaaggaagag cagcttttcc gcatcgacca ctacctcggc aaagagacga
     3841 tccaaaacat catggtcacg cgctttgcca acatcatttt cgatccgatc tgggacgcgc
     3901 gctatgttga ccatgtgcag atcaccgcag cagaaaccct cggtgtcgag ggccgcgccg
     3961 cctatttcga ccacaccggg ctgctgcgcg acatgttcca gaaccatatt atcgagatgc
     4021 tggctttgac ggccatggag ccgcccgaac ggttcgatgc cgacgcgatc cacgacagca
     4081 aactgaagct catccgcgcc atacgtcctt tcgataccgg cagcgacgcg aacgcgatcg
     4141 tgcgggccca atatagcgct ggccgcggta tgcccgctta ccgcgaagag gcgggcatac
     4201 ccacccaatc agagaccgag acctttgtgg cggcgaaact ctggatcgac acaccccgtt
     4261 gggcgggaac ccctttttac ttacgctcag gcaagcggct ggccgcaaac agcaacgcga
     4321 tcacgctcgt gttcaaacag gcgccccatc tggatctggg aggtcccggt gccaatgcgc
     4381 tcattttgcg ctcacgcccc ggtgagggca tgtgcctgca tctgctgggc aagcaccccg
     4441 gtcccaaact gtgcgtgggg acgctgccgc tctcgttcga ctatgccgaa cttgccccgg
     4501 gcaacgaggc cccggacgcc tacgcccgcc tgctgctgga cgccatgctg cacgaccaca
     4561 cgctgtttgt gcgcagcgaa accatccgca ccgcgtggga acttttcacg cctgtactgg
     4621 atttgtggcg tgactgtccg gaccggatgc cgctgcacac atatgaggcc ggctcggccg
     4681 ggccggccgc cgccgccgac ctgatggcag cggacggccg cgaatggtcc agcctgtaaa
     4741 gctgaagcaa gcaaccgtgc cgctacggct cgcaaacctt gtgaacggag gagtgagcgc
     4801 aatggcggag cggcggagag cgttgcctga tcaaacagat cggacggatc aggctgatca
     4861 ccaaatgaga gggcggcagg ggagcaggaa attacgcccg agcgatgatt accgccaaga
     4921 cgccacagac gccaagaggg atggaggagg cgattatggt ggagtggggg atgatgctgc
     4981 cggtgttgag tgaggctcac gctttcctgc cgggtcgtcg gtggattcgg tgatgccgtg
     5041 gtatcggaag taagcttcgg cgacctcgtt ccccatatag cggcgcaggg ttttcgggct
     5101 ggactgggaa aggtccacca tgatgaggcc gtctaccact gagctgaaat ccgggtccac
     5161 gttgaaggcg agcagtttgc cgctgagttt caggtattgg cgcagcagca ccgggatgtc
     5221 tttgccgtcg ggttcgatct ccgcgacgta gtccgccacc cggtcaatgt cgttgagata
     5281 ctccatatac tccttgaggc accactccga acggcgcggc ggtttgggcg ggaatttggg
     5341 cttcaccagc caggtcagct catccgccat gcaggtctgg cgcatggaag cgaggatcat
     5401 gcagcgtgag gcctcgcggt actcgttggt gatgctgacc gggccgaaca gcacacggta
     5461 gtgcgggttg cgcccgatga aggcggagat gcctttccac agcaggaaca gcgatgtgta
     5521 ggccttctgg tattcggtgc ggatgaagga gcgtcccatc tcgatggctg gcggaatgcg
     5581 cgtcatcagg cgctcgtcga atttgaacag tgtgcgcgta tagaggcctt tgattccgcg
     5641 ttccgtgaga atgcggtcgg tcaggccgag acggtacgag ccgacgatct cctgacgcgc
     5701 ggtgttccac atgaagaggt ggttataata ttcgtcaaag gcgtcgaggt cgatcgcgtt
     5761 gccggtgcct tcgccgacca cgcggaaggt ggtttcgcgc agacggccga tctcgcgcag
     5821 acaggagggg atctggtgcg ccgcggcgat gtagacggcg aagtccccgg ccgcaatcag
     5881 gcggcacgat tccggcaggg aggcgatctc gcgcgttagg gtctcgcacg gcacgggcgc
     5941 gatgatcgga tcggcgggga gcatcggcgg gttcagcgaa ataaagcggc gcgtgcggtg
     6001 cgtttcgcgc tcggccagca ggtaggaacg gaaccgcatg tagcggatga gctgcacgtc
     6061 atccagcgaa aacggctcca gcgcttttgg ggaaagcggg gttccgatgc gcacggtgat
     6121 tttccatccg gtcttgttgg cgatctgccg cggcagcagc gaggtgcgga aacgcggatg
     6181 caaaaacccc gctatctgga agaacacgtt gttgcggccg gggaaaaaca tcgggaccac
     6241 ggtggcgttg gttttgcgcg tcagcgcggc gatgcttgcg ctccaggcag ggtcgcgcac
     6301 acgcagggtg cggcggtcga aactggagac ctcgccggag gggaagacga tcaggaggtg
     6361 accttccttc agccaggaga gactctcctt gagggggcgc agattcgcct tggccgaatt
     6421 tttcgacccg aacggatcga ctaaaatgaa atcgccctgc atctccggaa tggcgccgag
     6481 caggtaattg gccatggctt taacgtcggg gcgcacacgc cgcagcaggg agaggaggat
     6541 gatcccttcg atgccgccga aaggatggtt cgcgaccacg acgaccgcgc cttctttggg
     6601 gatgcgggcc agatccgctt ccgaaaagtc ggttcggatt ccgatcgcct gcagggcgcg
     6661 gtcacagaag gggatgtctt tgggcatccg ccatgtgcgc gcatagatct tatcgcaggc
     6721 gcgcagaccg gtgatgcttt caaccggacg tttcacgatt ctcaccagca ggcgcaacag
     6781 gcgggtgtgg aaggtgtgga caggcagttc aaaaatacgg ggcgcctgat tttcggtgcg
     6841 gtcagtatcc atagtcagcc tccggagatt ttgggtggtg cgcgagtgcg agtacgaacg
     6901 cgagggggag tccgatgccg cggaccaaca gcgggtgcca cacggcggtt accgcgaacg
     6961 cggccaacgc gccagccgcg ccccatcgtc cggaacgggt cgccgcgcct agagcggacg
     7021 ccagcataag cagaaacgcg aagagcgcgg gcagaccgca cgatgcgccc aatacgaggt
     7081 acagattctg gatgtcgggt tccgcggggc cggtcgcatt cggaacctgt ccgtaaaagg
     7141 agccgatccg tttctgatag tgaccggggc ccacgcccag ccacggatgg gtcaggatca
     7201 tgccgacggc tgcctgccac tccggataac ggcgggtcgg gagtccggcc gaatcataca
     7261 aggcaaccga ttcgaaatgg caaaggtcgt tttcacgcgg aagattcttc aggacttcag
     7321 tctgccacag cattaacgcc agcatgacgg gcaggaacac gcgcgcgccg cgcgttgcgg
     7381 ctatcgcggc gatgacgacg acgacggccc agtaggaggc tccggagagg ttgaccgcaa
     7441 gaccggtcag cagcagcagc gcacaggcga tccgcgtcca gatggcgcgg cctgatgcga
     7501 gcgcgccgac cgcgcacagc ggcagggcca gcgcaaggaa gccgcccagc acattgcggt
     7561 tcccgaaggt gccgcccacc agcaacggac tttcccggcc gccgagcacg ttcacgatga
     7621 tatcgagcgt cttgggttcg gcgaacaggc ggatcgagtc gtggccttcc ggcgcgagat
     7681 attggatcac ggccaacccg agcaccacgg cggtcactgc gccgagcgcg atgaaaccgg
     7741 gcggcacacg agaggcggcg ggggatgccg ctccctctgc ggatgcgttg ctgaaaagcc
     7801 gttccttgcg gaggagcgcg gtgaacacaa cgggagcgat gataaaatat tcaactccct
     7861 gcacccattc tttgagcgcg tccatccggt ccgttgccgc ggacagcgcc gcgcccgacg
     7921 cgaggatgaa gagaaggtgc gtccagtgcg gccgcaggga aagcgccttt tttccgtccc
     7981 gcgccaacag gcactccagc acaaccagcg cggcggcaca ggccagaagc agatccgcca
     8041 ctccgatgcg caaagaggga atcagcgacc actgggtggg ggcggcgcac aatgcggcga
     8101 gggcgaaccc cgggaaggcg gcacgggaca acggtcgaat gtacgcgcgg gtcattggcg
     8161 cacacctgtt ttttcgatat cggtcgggac gaaacgccgg tcgctgctga gcaggatctg
     8221 gtcaagccgc acaccgtcct cgcggttcag aaaggtgagg gtgtgcctgc cccgggtgag
     8281 ccggagcggg ggggcggtgc gcgaaaccgg atatttgacc cagtgccagg ttttgtaggt
     8341 ggcgtcctcc ccgaacagga aaggtggctg gtcatccacc cgcacggtga acgagttgct
     8401 gcactcgccc tcccaccaga cgcggcacca gagggtgaaa accccgtctt ccgggatatc
     8461 cagatcaaag ccggccactc ccgcattgac cttgggcgga ttccccgcac cctccgcgat
     8521 ttcgagatat gcgccggagg ccccctcgat tccgttggag accacggtca cgggaggttc
     8581 gattcggttg gcggtttcgg cctcccagca gatccgcacc cctttcgcct gagcacggca
     8641 gatcaggaag agacccgcga gcagaatcat ccgttgcata gaagtcatag tccgcagtat
     8701 acggcattcc gtaccaatcg gcaacaggaa gggaggtcgt tcagggcctg cgggggcaaa
     8761 aaaaatggtg gcaaggaccg ggatcgaacc ggtgacacgc ggattttcag tccgctgctc
     8821 taccaactga gctacctcgc cacatgacgc atcgcaggaa gggatgcgaa atgggtgcaa
     8881 ctttagaaaa aaacgggggg gctgtcaatc cctctcgtgg cggactttcc gctgaatgcc
     8941 gagttgcggc ctgcgacatc tgatctgcga ggttcgggac ggttctctga attgttcacg
     9001 gcccgttcgg ggaacgggcc ctacctgaag agtgacttgg gaatgtgtcc ctttccaact
     9061 ccgccttgct tttcgcatcg tgttggcaca agatagagag gtgttggccc aatggattca
     9121 cggggagagg agtccgttgc gggcgactgg ttaatacggg aggatttcgg aatgcagtac
     9181 agaaagttgg ggtcgtcgga gattcaggtt tccgcggttg cgctcgggac atggtcgatc
     9241 ggcggcggcc cgtggtgggg aaagaccgat gacaacgact cgatccgcgc gattcaggcg
     9301 gcggtggatg cgggtatcac gctgatcgac acggcgccag cctatgggtt cggacgcagt
     9361 gaagaggtgg tggggcgcgc gatccggggg cggcgcgatc aggtggtggt ggccaccaag
     9421 tgcggcctct ggtggggtga cgacagggga gtcctccatt ttgagcagga cggatatacg
     9481 gtgcggcgct gtctcgatcc gcgcacgatc ggcgtcttga acggctcaac cgcctgcgcg
     9541 tgcgtctgca tgaggtcaat
//
LOCUS       DAOSRB010000110         9552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6920_length_9552_cov_6.566705, whole genome shotgun sequence.
ACCESSION   DAOSRB010000110 DAOSRB010000000
VERSION     DAOSRB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6920_length_9552_cov_6.566705"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..3605)
                     /locus_tag="PLZ74_07140"
     CDS             complement(72..3605)
                     /locus_tag="PLZ74_07140"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOR98132.1"
                     /translation="MTPSRKSIVLLSAALAVWAAFAQSVIEIEMPWPKEGTGFPCGHP
                     VDVSIRHTLDGKATAAKGVKIAFDNFGPKTVRAEETCDLPEQGLRVKGLTLNEPGFLR
                     CRVTVGGKNYVQSVPFEPERIVKGSPTPPDFMTYWQREIAKLDAESSDEPELKPWRGP
                     DTNQWDAWRVSFATAGGKRVYGLLSVPKNLSEGPFPVHVSVPGAGQPSKTYSASWIYS
                     QTPERNTVRLTMFAHCAPIDDAKEREAYFTRIRAEHAEKYGVGYYPQAGISESREAYH
                     FHPIILGANRAVNWLARQSYCDTNRFTYSGSSQGGGFGIILAGLNRHFTRVFADVPAM
                     ADTMGARAGRQSGWPQPVETQRPENRSAAERFAPYFDGANFARYVTCPIRVTCGCKDT
                     SCAPPAVYAAYNEIPSQDKQIFVMPHNGHESNGKIKNQAHAWRQGTGERGLAPFDTLT
                     CEVTGVAFGSGANTDPTFKVPKLVAAIRRTDGVIEDVPLRAVNWMGAYLVDRRFSKSE
                     RERFGTKAELVGVRLEGGAPETNAKIKIENATMFKEVLNPLSIQVTPRDALPFPNRAD
                     TIVPDTPEPAAGDLLAEWNASLPPGCAVSKRRVGKSLVIDITAPAGKVTEIPLGVARE
                     AKKVKSFAIPYLTWGERNGRVHADLLEGGWFRLAEFDWYASNASDVYARETEGGRELV
                     ARYKPKTDGTFNPVRERIVITLSNVFEEILPEIPNPASPWKHVTGMHVWRSHPSYDRA
                     EDLALWRQVKAAGIKHVAVTDHETMWRDGGEPFTFCTDAAKGKGGDEAQKAYTRAMID
                     ELGFLYGPYNNFTDLATQSEFFNRDTVGRWSDGSFHNAWMRCYGPKPVLAPLACDRFA
                     GAIQRKFGFNTAYCDVHTSMLPWQRTDYDHRVPGAATYAQTYYSWGETLLKQKKIWNG
                     PVYSEGAHHFMWAGLADGSYAQDRSYDFRNEPWIVEFELLRTQRLCTDFGMGTLSMFS
                     PARTLHERLYYLPYQPQGRDELVNRFLAATIAFGHSGYLILDKCWEPDKPFGRAYGVK
                     SKPVWQEKGLPAETLRSYFMIQALAARYTQSHAVEIRYVDAEGRLHAASEAIRNGTVA
                     RNQIVTRYADGTVTAVNGSDTLPMKTSAHGRKIELAPNGFAGWSSGVTVLSTDTDGRR
                     VHYAEGPDYVYREIEGRKPCVSLTSRHETKP"
     gene            complement(3616..5610)
                     /locus_tag="PLZ74_07145"
     CDS             complement(3616..5610)
                     /locus_tag="PLZ74_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98133.1"
                     /translation="MRTVLIVSVALGVIGAFADDQWTLNSDTPTWWFEPAAWSNGTYP
                     QSGESVIIGASSDNAKANVYLQSGDVVSGIADLTFGKNTGAQTHLTIPTGASLTFTNT
                     MRLMGQSVQKATGLVEITGGTLKGKAFNWGGHTSSKGTVRITNNGRAEFTSCTSGWLG
                     PSDFEIDTGHLCVGSGNFLFGNRTDAYARLFGRNATIETTAGIFSIGANVSGALGAWF
                     LEDSAVTVSGSGGIVLGRASQSCLRITNTVVRFTHASAPFTLGQDTNNTVTAGVWSDF
                     EMVGENAMVSNSYRLKLFNNARYIQRGGQTILRANNTGDQIEINGVPGTEQRVEVHGG
                     TFSLTNANPSGDARIILGGSARAMFRQTDGTVTAPGILFKPCGPDNARYEMTGGTLEL
                     DDSKGGNGGIRTSDKSTRAGTFSIIGGSPNIRMTRIGTYNSTDYQPMIEYVICTNFVA
                     PICFSRPSQSYYSWVSGIYTIVPQGGMQLIHTNTVMLLDGRDGEFGILPQNPGSGGTF
                     LPNPALWESGTTDLYGGSKADRLGSRLLASAAFESGAAPATPVSCGFLKLPKTSQKGY
                     TVWLNLLPQNEVTIAELVADLTAAGYPAQADASREGYNVRAAIPENLVAHGSDDEYLV
                     FDFARHMTPEDVRNGTPTVRALVGRVLAEKRTGGFAILIN"
     gene            complement(5946..6611)
                     /locus_tag="PLZ74_07150"
     CDS             complement(5946..6611)
                     /locus_tag="PLZ74_07150"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR98134.1"
                     /translation="MQKLFLLALALTCAVTVRSEMLVKSEPIRPDRKITLFNGRDLSG
                     WTKVITAEPGACPDATWSVADGVIRCTGKPFGYLMTQQSYADYRLHVEYRWYGTSDQM
                     NSGIFIHKTGPDTFFLPKAIETQLKKDNAGDFVLLSQATLNNLTNPKNLRVEKIAPCS
                     EKPVGEWNRVEITVRGNTLSSYINGVLQNSGNAIYTDAGQICLQSEGGPIEFRNITIE
                     PIP"
     gene            complement(6937..7770)
                     /locus_tag="PLZ74_07155"
     CDS             complement(6937..7770)
                     /locus_tag="PLZ74_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOR98135.1"
                     /translation="MESLLPNYDGVNIEWDQRSIHSEQVTFGQVRYQPGGYCGPRVQR
                     DFELALLYSGSCCVRLDGQPRELRVGHVYLFKPRRREHFLFDPQTPTHHFWCSVTPGY
                     LPPALRHALEATPDEGHLPSECFNRLISAAFLLRTVQSPTSLQVVDTLALTLFWEFLN
                     LADHDTLMIREDACVTRALRYMEEHLGESDCFVGAQRAARCSENALIYKFRRALGTTP
                     ARHLWRLRTEKGLALLAETGLSVTEIADQCGFKNPFHFSRCVRRMQGQPPREIRRRAW
                     S"
     gene            7994..>9552
                     /locus_tag="PLZ74_07160"
     CDS             7994..>9552
                     /locus_tag="PLZ74_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98136.1"
                     /translation="MSMFMGKTPCSGCRLERRDFLAAAGCTACAATLGRVMAAAPARA
                     AEKAPDLRIRILFALHAPVQPKPDWPNIGFNFQPVMDQFVEGLRTHCPGFDFVTSMAA
                     NEEEAKAIVAADQGSRIDGYIVYQLNCWNRVVQPAIATGKPTLFVDFAYGGSGGMLVY
                     TSGFLIYKVPNVGFVSSSRFEDVAAAVQCFAQVKRGGTDFATLTAACRRKLTPQAGDL
                     SCVNDPVEVLSAKDCLAKMREAKLLVVTNKSVGPYPAIKEQMGIEVVQIPFEELNTAW
                     KAADKDQARAVADRWQKDARAIIGVSRETLENSAAMYLGQKALLKKHGANGLTVNCLG
                     GFYGGHIHAYPCMGFHELVNEGLIGGCEADVRSAVSMIAMTTLTQGRPGFISDPVIDT
                     SKRQIIYAHCVASNKPFGPQGPSNPFEIMTHSEDRKGAAVRSLFPVGYMTTTVEFDAN
                     RKTVLFHQAKAVANVDDDRACRTKLAAEPVGDMEKLFTHWNRWGWHRVTVFGDLKEPV
                     FALADAMGWKVV"
BASE COUNT         1818 a         2788 c         2993 g         1953 t
ORIGIN      
        1 aatcggggtc ggggtcggga tcgctatcgg gatcgattct tccaccacgg gacacaagaa
       61 cacacggaaa gtcacggctt tgtttcatgc cgcgacgtga gcgagacgca cggctttcgg
      121 ccttcgatct cgcgatagac atagtcggga ccttcggcgt aatggacgcg gcgtccgtcg
      181 gtatcggtgg agagcacggt gacgccgctg gaccatcccg cgaaaccgtt gggcgcaagc
      241 tcgatcttgc gtccgtgcgc ggacgtcttc atcggcaaag tgtcgctgcc gttcactgcg
      301 gtgaccgtgc cgtccgcata acgtgtgacg atctgattgc gggccacggt tccattccgg
      361 atcgcctcgg aggcggcatg taaccgcccc tcggcgtcca cgtaccggat ctcgacggcg
      421 tgcgattgcg tgtaccgggc cgcgagcgcc tgaatcatga agtaactgcg gagcgtctcg
      481 gcgggaaggc ccttttcctg ccagaccggt ttgcttttta ccccgtatgc gcgcccgaac
      541 ggcttatccg gctcccagca tttgtcgagg atcaggtaac cggaatggcc gaacgcgatt
      601 gtcgcggcga ggaaacggtt cacgagttcg tcacgacctt ggggctggta cggcagataa
      661 tacagccgtt cgtgaagagt tcgcgcaggc gaaaacatcg agagcgttcc catgccgaag
      721 tcggtgcaaa gacgctgtgt gcggagcagt tcgaactcga cgatccatgg ttcattacgg
      781 aaatcgtagc tccggtcctg cgcgtaggag ccatccgcaa gaccggccca catgaagtgg
      841 tgcgcccctt cggaatagac gggaccgttc cagatcttct tctgtttcaa gagcgtctcg
      901 ccccacgaat aataggtctg cgcgtaggtt gccgcgccgg gaacacggtg gtcgtagtcc
      961 gtgcgctgcc acggcaacat cgaagtgtgc acgtcgcagt acgctgtgtt gaaaccgaac
     1021 ttacgctgga tcgcgccagc gaaccggtcg caggcgagcg gtgcgagcac gggtttcgga
     1081 ccgtagcacc gcatccaggc gttgtggaac gatccgtccg accatcgtcc caccgtgtcg
     1141 cgattgaaga attcgctctg cgtcgcgagg tccgtgaagt tgttgtacgg accataaagg
     1201 aagccgagtt catcgatcat cgcgcgcgtg taggccttct gtgcctcgtc gccgcccttc
     1261 ccctttgccg cgtcggtgca gaaggtgaac ggttcgccgc cgtcccgcca catcgtctca
     1321 tggtccgtca cggcgacgtg cttgattccg gcggccttta cctgccgcca gagcgccaga
     1381 tcttccgcac ggtcgtacga tgggtgtgaa cgccacacgt gcatcccggt gacgtgtttc
     1441 caaggggacg ccggattggg gatctccggc aggatctctt cgaaaacgtt cgaaagcgtg
     1501 atcacgatcc gctcacgcac ggggttgaac gtcccgtccg tcttcggttt gtaacgcgcc
     1561 acgagttcgc ggccgccttc cgtctcccgc gcgtacacgt cgctcgcgtt cgaggcgtac
     1621 cagtcgaact ccgccagccg gaaccagccg ccttcgagca ggtctgcgtg cacgcgtccg
     1681 tttcgctcgc cccaggtcag ataggggatg gcgaagcttt tcaccttttt cgcttcgcgt
     1741 gcaacgccca gcgggatttc cgtgactttg cccgctggag ccgtgatgtc gatcacgagt
     1801 gatttcccga cgcgccgttt cgacaccgcg cagcccggcg gcagcgaggc gttccattcc
     1861 gcgagcagat cgcccgccgc cggttcgggc gtgtcgggaa caatggtgtc cgcacggttc
     1921 gggaacggca gcgcgtcacg cggcgtcacc tgaatcgaca acggattcag cacctccttg
     1981 aacatcgtgg cgttttcaat cttgatcttg gcgttcgttt ccggcgcgcc gccttcgagc
     2041 cgcacaccca ccagttcggc ctttgttcca aaacgctcgc gttccgactt cgagaaccgc
     2101 cggtccacca gatacgctcc catccaattg acggcccgca gcggaacgtc ctcgatgaca
     2161 ccatccgtcc gccggatcgc tgccacgagc tttggcactt tgaacgtcgg atccgtgttg
     2221 gcgccggatc cgaacgcgac gccggtcacc tcgcatgtga gcgtgtcgaa cggcgccagt
     2281 ccacgttccc ctgtgccttg tcgccaggca tgcgcctgat tcttgatctt gccattgctc
     2341 tcatgcccgt tatggggcat gacgaagatc tgtttgtcct gagatggaat ctcgttatag
     2401 gcggcgtaaa cggccggtgg cgcgcagctc gtgtctttgc aaccgcaggt gacgcggatc
     2461 gggcaggtga cgtagcgcgc gaaattggcc ccgtcgaagt acggggcgaa cctctctgcg
     2521 gcggaacggt tctcggggcg ctgcgtttcg acgggttgcg gccagccgct ttgtcgtccc
     2581 gcgcgtgccc ccatcgtatc cgccatcgcc ggaacatcag caaagacgcg tgtgaaatgg
     2641 cggttgaggc ctgcgaggat gatgccgaag ccccccccct gcgaacttcc ggagtaggtg
     2701 aagcgattcg tgtcgcaata ggactgccgc gccagccagt tcacggcgcg gttggcgccg
     2761 agaatgatcg gatggaaatg atacgcttcg cgcgattcgg agatgccggc ctgcggataa
     2821 tatccgaccc catacttttc ggcgtgctcg gcccggatgc gcgtgaaata cgcttcgcgt
     2881 tccttggcgt cgtcgatcgg tgcgcaatgc gcaaacatcg tgaggcgcac cgtattccgc
     2941 tccggcgtct ggctgtatat ccagctcgcc gaatacgtct ttgagggctg gcccgcgccg
     3001 ggaacgctca catgcacggg gaacgggcct tccgaaaggt ttttcggcac ggacaacagt
     3061 ccgtagacgc gcttcccgcc tgccgtcgcg aagctgaccc gccaggcgtc ccattgattg
     3121 gtgtccggac cgcgccacgg tttcaactcc ggctcgtctg acgactccgc gtccagtttg
     3181 gcgatctcac gttgccagta cgtcatgaaa tccggcggcg ttggcgatcc cttcacgatc
     3241 cgttccggct cgaacggcac ggactgcaca taatttttac cgccgaccgt gacccggcat
     3301 cgcagaaagc cgggctcgtt cagcgtgaga cccttgacgc gaagaccttg ttcgggaaga
     3361 tcacaggttt cttccgcacg cacggttttc ggcccgaagt tgtcaaaggc gatcttgacg
     3421 cctttggcgg cggtcgcctt gccgtcaagg gtgtggcgta tcgatacgtc gacggggtgt
     3481 ccgcacggga agcccgtccc ctcctttggc caaggcattt cgatctcgat aaccgattgc
     3541 gcgaatgctg cccatacggc aagggcagcg gacagcaaca cgatgctttt acgggagggt
     3601 gtcatgtttg cttcctcaat ttataaggat ggcgaatccc ccagtccgct tttcggccaa
     3661 aacgcgtccg acgagcgcgc ggacagttgg cgtgccgttc cggacgtctt ccggcgtcat
     3721 atggcgggcg aagtcgaaga caaggtattc gtcgtcggaa ccgtgcgcga caaggttttc
     3781 cggaatcgcc gcccggacgt tgtacccttc gcgggacgca tccgcttggg cgggatatcc
     3841 ggccgcagtc aaatcagcaa ccagttccgc gatcgtgact tcgttctgcg gcagcaggtt
     3901 cagccagacc gtgtagcctt tttgagacgt cttcggcagt tttaaaaacc cgcacgagac
     3961 cggtgtggcg ggcgcggcgc ccgattcgaa cgccgccgaa gcgagaaggc gcgaaccgag
     4021 ccgatcggcc ttgctgccgc cgtagaggtc tgtcgtgccg gactcccata acgcggggtt
     4081 cggcaggaac gtgccgccgg aaccggggtt ttgcggcaag atgccgaact cgccgtcgcg
     4141 tccgtccaac agcatcaccg tattggtatg gatgagttgc atgccgccct gcggcacgat
     4201 cgtgtagatg ccgctcaccc aactgtaata agattgcgac ggacgcgaga aacagatcgg
     4261 cgcgacgaag ttggtgcaaa tgacgtattc gatcatcggc tggtagtcgg tcgagttgta
     4321 cgtgccgatg cgcgtcatcc ggatgttcgg cgaaccgccg atgatcgaga acgtgcctgc
     4381 gcgcgtggat ttgtccgagg tgcggatgcc gccattgccc cccttggagt cgtcgagttc
     4441 gagcgtgccg ccggtcatct cgtaccgcgc gttgtccggg ccgcagggct tgaaaaggat
     4501 tcccggtgcg gtcaccgtgc cgtccgtctg gcggaacatc gcccgagcgc tgccgcccaa
     4561 gatgatgcgc gcgtcgccgc tcggattcgc gttggtgagc gaaaacgtgc cgccgtgcac
     4621 ctcgacacgt tgttccgtgc cggggacgcc gtttatctcg atctgatcgc cggtgttgtt
     4681 tgcccggagg atcgtctgcc ccccgcgctg aatgtaccgc gcgttgttga acagtttcag
     4741 ccggtacgaa ttcgagacca tcgcattctc gccgaccatc tcgaagtcgg accacacgcc
     4801 cgcggtcacc gtattgttgg tgtcctgtcc gagggtgaag ggcgcggacg catgtgtaaa
     4861 gcggactacg gtgttggtga tgcggaggca ggattgcgaa gcgcgcccga gcacgatgcc
     4921 gccactcccc gagacggtga cagcagaatc ttccagaaac cacgctccaa gagcccccga
     4981 tacgttggcg ccgatagaga atatccccgc cgtggtttca atggtcgcgt tcctgccaaa
     5041 caaccgggca tacgcatcgg tacgattgcc gaaaaggaaa ttgccggatc cgacacacag
     5101 gtgaccggtg tcgatttcga agtccgacgg accgagccat cccgacgtgc aggaggtgaa
     5161 ctccgcgcgg ccgttgtttg tgattcggac ggtacccttc gacgatgtgt gtccgcccca
     5221 gttgaacgct ttgcccttga gcgtgccgcc ggtaatttca accagccccg tcgctttctg
     5281 gacagactgc cccatgagtc tcatcgtgtt cgtgaaggtc agcgaggcgc cggtcggaat
     5341 cgtgaggtgg gtctgcgctc cggtgttctt gccgaaagtc aggtcggcta tgccggaaac
     5401 cacgtcaccg gattggagat agacattcgc tttcgcgttg tccgacgatg cgccgattat
     5461 cacggactcc ccgctttgcg ggtaggtgcc gttcgaccac gcggccggct cgaaccacca
     5521 tgtcggcgtg tcggagttga gcgtccattg atcgtcagcg aatgccccga tgacgccgag
     5581 tgctaccgaa acgataagaa cggtccgcat gataatttcc ccctatgcta aaagcccccc
     5641 ttttccatta actgtgtaaa accataacac acccccccct tgcgtcacaa gaaacgtttg
     5701 tgcgaacgca ttcctgaccc cctgaccgtc cgtactttgt tcggctcgca gaaccgccct
     5761 caccgtcaaa cagacatttg cctctcccgg acgccgcgac cggcacacat acacgaaacc
     5821 gagtacgaga acgaggagga ggacgggcgc tgatcccgat aacgatgcct cagaccggga
     5881 caatcgtcgg acgggacggg cagacgctcg tcccgcccag ccacaatatc gggaggggtg
     5941 ccggatcagg ggatcggctc gatcgtgatg ttgcggaact cgatggggcc gccctcactc
     6001 tggaggcaga tctggccggc gtcggtatat atcgcgttgc cgctgttctg caagacgccg
     6061 ttgatgtatg aagagagggt attgccccgg accgtgattt caacgcggtt ccattcaccg
     6121 accggtttct cggagcatgg ggcgattttt tcgacgcgca ggttcttggg gttggtcagg
     6181 ttattcaagg tggcttgcga gagcagcacg aagtcccccg cgttgtcttt tttaagttgc
     6241 gtctcaatgg ccttaggcag gaagaaggtg tccgggccgg ttttatggat gaagatgccg
     6301 ctgttcatct ggtccgaagt cccgtaccag cggtattcaa catgcagccg gtagtcggca
     6361 taactctgct gcgtcatcag gtagccgaac ggcttccctg tgcaacggat cacgccgtcc
     6421 gccacggacc acgtggcgtc cgggcaggcg cccggttcag cggtgatgac cttggtccat
     6481 ccggagagat cgcggccgtt gaacagcgtg attttccggt cgggccgaat gggctccgat
     6541 ttgacaagca tttcgctgcg gacggtgacg gcacaggtta aggcgagggc gagcagaaat
     6601 aacttttgca tggttccggt ctcctaattg tgaggtcgtg tggtgtgcag tgtcccatct
     6661 tcccgtgacg ctgtcaacgg ggtatgacgg ttcccgggtg attagcccgc cgcccgcgct
     6721 tcgcacggga atgcaaatga ggataacttt gggtgtaagt tataggcaag cccgccgtgc
     6781 gccggagccg cgagaaaact ttgggatgcg tttttatgac gggatgacag gattttcagg
     6841 attaaaaaaa tcacgttaat cctgtaatcc tgtcaattta taggcaagga gtttcgtaaa
     6901 gggcacgcaa tttcgatacc ctgcatcggg ccggggtcag gaccaggcgc gacggcggat
     6961 ttcgcgcggc ggctggccct gcatgcgccg aacgcagcgc gaaaagtgga acgggttctt
     7021 gaacccgcac tggtcggcga tctctgtcac cgacaggccg gtctccgcca gcaacgccaa
     7081 gcctttttca gtacggagcc gccacaggtg gcgtgccggt gtggtgccga gtgccctgcg
     7141 gaacttgtag atcagcgcgt tttccgagca gcgcgccgca cgctgcgcgc caacaaaaca
     7201 atcggattct cccagatgct cttccatgta acgcagcgcc cgggtcacgc aggcgtcctc
     7261 acggatcatc agggtatcgt ggtctgcgag attcaggaac tcccaaaaga gcgtgagcgc
     7321 caaagtgtca acaacttgca gcgaggtcgg cgattggacc gtgcgcaaca ggaaagcggc
     7381 ggaaatcaga cggttgaaac attccgaggg gagatgcccc tcatctggcg tcgcctcaag
     7441 ggcatggcgg agcgccgggg gcaggtaacc gggcgtcacg gaacaccaga agtggtgcgt
     7501 cggggtctgg ggatcgaaca ggaagtgttc gcggcgccgg ggtttgaaca gatagacgtg
     7561 cccgacgcgc aactcccgcg gttgcccgtc aagccgaacg cagcaggacc ccgagtaaag
     7621 cagcgccagt tcgaaatcac gttggacgcg tgggccgcaa tatccgccgg gctggtaacg
     7681 aacctggccg aacgtgacct gttctgagtg gatactgcgc tgatcccact cgatgttgac
     7741 accatcatag ttagggagaa gactttccat gagcggatcg aacatagcag agaagaacgg
     7801 acttttgcaa gacgttttgt gacggcggat atgttatttt ggattagggg tattcactaa
     7861 gcttgcgaaa aggggtatat taaaaaggaa atgtgacatt gggtatgttc caatggaaga
     7921 gcgggaaagc cgggttcacc cggcttcgca aggctgatcg gaaaagtgag tcatcagaaa
     7981 aaggaggctg agaatgagta tgtttatggg gaaaacaccc tgttcaggtt gtcgtctgga
     8041 acgtcgcgat ttcttggcgg ccgcaggctg tacggcctgt gccgcaacgt tgggacgtgt
     8101 gatggcggcc gctccggcga gagcggcgga aaaggcgccc gatttgcgta tccgcatcct
     8161 gtttgccctc catgcgcctg tgcagccaaa accggactgg ccgaacatcg gatttaattt
     8221 ccagccggtt atggaccaat ttgtggaggg gttgcggacc cattgcccgg ggttcgattt
     8281 cgtcacgtcg atggcggcca atgaggagga ggcgaaagcg attgtcgccg cggatcaagg
     8341 cagccggatc gacgggtata tcgtctatca gttgaactgc tggaacaggg tggtgcagcc
     8401 ggccatagcc acgggcaagc cgaccttgtt cgtcgatttc gcctacggcg gcagcggcgg
     8461 aatgctggtg tacacctccg gttttctgat ctacaaggtg ccgaatgtcg gttttgtctc
     8521 ttcctcccgg ttcgaggatg tcgcggcggc ggtccaatgt ttcgcgcagg tcaaacgtgg
     8581 cggcacggat ttcgcgacgt tgactgcggc gtgccggcgg aagttgacgc cgcaggcggg
     8641 cgacctcagt tgtgtgaatg atccggtcga ggtgctttcg gccaaggatt gcctggcgaa
     8701 gatgcgggaa gccaaactgc tggtggtgac aaacaagtcg gtcggaccct atccggcgat
     8761 caaagagcaa atggggatcg aggtcgttca gataccgttt gaggaactga acacggcatg
     8821 gaaggcagcc gacaaggatc aggcgcgcgc cgttgccgac cgctggcaga aggacgcgcg
     8881 cgcgattatc ggcgtttcgc gggagacgct tgaaaactcg gcggcgatgt atctgggtca
     8941 aaaggccctg ctgaaaaagc atggggcgaa cgggctcacc gtcaattgcc tcggcggctt
     9001 ttacggcggc cacatccacg cctacccctg catgggcttt cacgagttgg tcaatgaggg
     9061 gttgatcgga gggtgcgagg ccgacgtgcg ctcggcggtt tccatgatcg ccatgaccac
     9121 gttgacccag gggcgccccg gtttcatttc cgatccggtg atcgacacgt caaagcggca
     9181 gatcatctac gcccattgtg tcgcctcgaa caaacccttc gggccgcagg ggccttccaa
     9241 cccctttgag atcatgacgc attcggagga ccgcaaaggc gcggcggtgc ggtcgctgtt
     9301 cccggtgggg tacatgacca cgacggtcga gttcgatgcg aaccggaaaa cggtcctttt
     9361 tcatcaggcc aaggcggtgg ccaatgtgga cgatgaccgt gcctgccgca cgaaactggc
     9421 cgccgagccg gtcggcgaca tggagaaact gttcacccat tggaaccgat ggggatggca
     9481 ccgcgtgacc gttttcggcg atctcaaaga accggttttt gcgctggccg acgccatggg
     9541 ctggaaagtc gt
//
LOCUS       DAOSRB010000111         9549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_6923_length_9549_cov_6.771540, whole genome shotgun sequence.
ACCESSION   DAOSRB010000111 DAOSRB010000000
VERSION     DAOSRB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9549
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6923_length_9549_cov_6.771540"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..743
                     /gene="gatC"
                     /locus_tag="PLZ74_07165"
     CDS             447..743
                     /gene="gatC"
                     /locus_tag="PLZ74_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOR98137.1"
                     /translation="MKDTINVAYVAELARIELTPEEQQLFQRQLETVVRYVEKIGRLE
                     LSGIEPTQHGQNLVNVFREDQTVAAESEARELFLANAPERNGYEFRLPKIVEEA"
     gene            799..2235
                     /gene="gatA"
                     /locus_tag="PLZ74_07170"
     CDS             799..2235
                     /gene="gatA"
                     /locus_tag="PLZ74_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOR98138.1"
                     /translation="MDVAGLTLCAAAEGLRKKTYSSVELTRAVLDAIREKDGALHAYL
                     SVDEEGALRQAAAADAARAAGHDTDLLGIPVAIKDLINVEGQPCTCASNMMQGYVAPY
                     DATVAARLRAAGAVFAGRTNMDEFAMGSSSETSAFKVTRNPHDLERVPGGSSGGSAAA
                     VAGGEAIAALGSDTGGSIRQPAAFCGCVGLKPSYGRVSRYGVTAYASSLDQVGPMTRT
                     VEDAAVLLQTLAGVDPHDGTTLDTPVPDYRAALVGGVRGLRIGLPKEYFVDGTDPEVM
                     ACVRRAVDACATAGAEIVEVSLPHTEYAVAVYCIVGTAEASANLARFDGVRYGHRTSR
                     PASVFDLYARSRAEGFGQEVKRRIILGTYVLSSGYYDAYYGRAQKVRTLIRRDFTEAF
                     ERCDVLFTPVAPTPAYRIGERCEDPLTMYLGDTFTVPASLAGICGLSVPCGRTAAGLP
                     VGLQILGPAFGESVVLRAGQAVEQEVRA"
     gene            2232..3671
                     /gene="gatB"
                     /locus_tag="PLZ74_07175"
     CDS             2232..3671
                     /gene="gatB"
                     /locus_tag="PLZ74_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOR98139.1"
                     /translation="MSRYLVTIGLETHVQLKTLTKMFCGCSLTFGEEPNTNVCPVCLG
                     YPGALPVMNREAVRLTVLSGLMLGCEINRHSTFDRKNYFYPDMSKDYQISQNTHPLCL
                     GGGVTIETPEGPKRIRINHIHLEEDAAKINHYAQFSGVDFNRCGTPLMEIVSEPDLAS
                     PDEAMAYLLALRQQLVYAGVSDCNLEEGNMRSDVNISIRPEGQTEFGTRVEIKNMNTF
                     KGIHAALKYEIARQLAVVRGNGTLVQETRRWDPEQGETQSMRSKENAHDYRYFPEPDL
                     VPVALTDVQIEAWREQLPEAPAARRERMMAEYGLPAYDAGVLADIRENADFFEAAVRA
                     SKPGMGKAVSNWFMTEVMRLLAETGQPVGACALTPAALAELVQLVEEGVINGPTAKEL
                     LPELFHEGGMPAARVEARGLAQVSDDAALHAFIDQVFRENGKSVEDYRAGKKAAAGFL
                     VGQVMKVSHGKADPKRVGRLVAERLATRT"
     gene            3703..5397
                     /locus_tag="PLZ74_07180"
     CDS             3703..5397
                     /locus_tag="PLZ74_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOR98140.1"
                     /translation="MTSNDDYVVQLLREQSYVSAEQVEAVANSIKQEGETTLDILVQT
                     GIVTEDDVLSLVAAQFGMEYVHVNPDAIDPAVTEVVPADVARKYGVVPVTQTEDTVTV
                     ALSDPMAYDTVDSLRYVLKMNVEAVVAPRSEVRAAVDRLYPESFESIYGAMSDSEIDV
                     AEKAKSDTGQESDDSDAPVIRLVSMIITEACKMRASDIHLEPMERTFRVRYRIDGVLR
                     QMDTPPKRLQAAILSRVKIMSNMKISEKRVPQDGRIQIKVQGRDLDLRVSSVPTNHGE
                     SIVMRILDKSNLSLGLPQLGFLSDDQNKFERLISLPDGVLLVTGPTGSGKTTTLYACL
                     GQINLPDRKIITVEDPVEYQMSGINQVQVNKDVGLDFSAALRSILRQAPNIVMIGEIR
                     DHETADIAMEAALTGHLVFSTLHTNDAPSAVTRLLDIGVKPFLVASALRAAMAQRLVR
                     AVCEKCRAEYKPTERELKMLGAMAKNVTVSQMYKGTGCSACGQTGYKGRKGIFEIFQI
                     DDTIQRLIFDHSPATVLRTRAREMGMRTLREDGLLKVASGMTTLEEVLRATMGDAD"
     gene            5397..6485
                     /locus_tag="PLZ74_07185"
     CDS             5397..6485
                     /locus_tag="PLZ74_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOR98141.1"
                     /translation="MSTEINMTELLQATVDEGASDLHIRVGIPPMLRIHGALTPLDSP
                     PLTPQDTESLARMITAEDKLNEIQECGGADFALPFGDLARFRVSVFKERGHFGIVLRQ
                     IPSKLLTLEQIGLPEQVKELLFRPRGLILVTGPTGSGKTTTLASMIDIINRERDVHII
                     TVEDPIEYYHPHKKSVVTQREIGHDVTSFAEAIRRALRQDPDVILVGEMRDLETMQAA
                     ITAAETGHLVFATLHTTGAAATVDRIVDAFPTDQQEQVRTQMSVGLVAVISQLLLARV
                     DKPGRVAAFEIMISTPSIRSRIRDNKTFQISSDIQTGAKFGMISLDSHLMDLYLGGLI
                     SYDDLVTKSQDPDTLVQKLKDELQSRRN"
     gene            6485..8203
                     /locus_tag="PLZ74_07190"
     CDS             6485..8203
                     /locus_tag="PLZ74_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOR98142.1"
                     /translation="MAVTSTSVAPPYHPLIDLLVSNGIIDQQQAEELRDEQKNSGKPF
                     RTLLVDAGYVSEDDLLGMMAAYQGCDVIDLEMMSLDTEVIQSIPASVARMYNVLPVSA
                     TAGTITLATSDLVDPHVTDELMFVLTRDVQFVMARENDVRTRVAEYYGDESATVADML
                     NSLESDLKLDTSLDVDDTDDKSLESAASAAPVIRFVNLVLYQAVTDRASDIHFEPFET
                     DFKIRYRVDGALYEMSPPPRRLALPIISRIKVMSGLNIAERRVPQDGRIALTVAGRSV
                     DLRVSCLPTAHGESVVLRVLDRSVVSLDLENVGLPEDVYEELTLDIEKPNGIIIVTGP
                     TGSGKTTTLYSCLRRINTIDQKLLTAEEPVEYDMDGIVQVQINPSAGNTFPRVLRAFL
                     RQDPDIMMIGEIRDLETAEIAVQASLTGHLVFSTLHTNDAAGAVTRLIDMNVAPYLIA
                     STLEAVLGQRLVRTICLNCKEPYTPDDETLSRMDLKRAAVGDRPFYYGRGCSKCNGTG
                     YKGRKGVFEYLRVSDPIRELINERRPTLFIRERARELGMRTMREDAIRNVLDGYTTVD
                     EVLRYT"
     gene            8257..9162
                     /gene="trxB"
                     /locus_tag="PLZ74_07195"
     CDS             8257..9162
                     /gene="trxB"
                     /locus_tag="PLZ74_07195"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOR98143.1"
                     /translation="MVIIGSGPAGLTAAIYAARANLNPVLIEGMQPGGQLTQTTDVEN
                     FPGFENPIDGTTLVMTMRKQAERLGVRCLADEVSACALAAKEKTVTLMLGGELQTRAV
                     ILATGASARYLGLESEQRLIGKGVSACATCDGSFFKGQPVAVIGGGDTAMEDALYLSR
                     IAERVTVVHRRDTFRASKIMAGRVLASPQIEVAWNSVVDEVLGAEEVTGLRLRHVHTG
                     ALTEIPVNGVFVAIGHTPNTALFAGQVELDPEGYVVTRNTRTSLPGVFAAGDVQDRHY
                     KQAVTAAGSGCMAALEAERFLASGE"
     gene            complement(9175..>9549)
                     /locus_tag="PLZ74_07200"
     CDS             complement(9175..>9549)
                     /locus_tag="PLZ74_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98144.1"
                     /translation="SAASSARLYWESFGPGKRKPLPVTVPTGVAVYPQEIVPPVRRWM
                     EAAGFNQIRHWQAMPRGGHDLLNRIHVPNDRLHHLINRQHGTRRDRSGQQTGQQAEAI
                     FPRITVKPSVGFGKRILFIKVK"
BASE COUNT         1857 a         2622 c         3258 g         1812 t
ORIGIN      
        1 aagccaggat tacaggatta acaggatttc tgattgaggt tatcggtagg ccggctgtcc
       61 ggggttctgg gcgagaacgg ggtggtgacg gggacgttaa actttaagcc aggattacag
      121 gattaacagg atttctgatt gaggttatcg gtaggccggc cgtccggggt tccgggcgag
      181 aacagggttg tgacgggaac gcttaacagt atgcagtgaa gaggggagag ggaggtgcgc
      241 gggcggatgc ccgctggcgg gtgttaaggc tgtggggctg taagggaggg gcaagcagac
      301 gcttgcccct cccgatctga gacatcgcta tcggatcgat acgttcaaac gtatttgaat
      361 tgtaccttca ggctggaaaa ggtaaaatag aactctttct ttttacaggt gccggaggcg
      421 gacgaaagcc caggaaggaa ccgcaggtga aggacacgat taatgtggcg tatgtggccg
      481 aattggcgcg gatcgaactg acgccggagg agcaacagtt gtttcagcgg cagttggaga
      541 cggttgtgcg ttacgtcgaa aagatcggaa ggttggagct ctccggcatc gagccgacgc
      601 agcacggcca aaatctggta aatgtgttcc gtgaggacca gacggtcgcg gcggagtcgg
      661 aggcgcgcga gctgtttctg gcgaacgcgc cggaacgcaa cgggtatgag tttcggttgc
      721 cgaagattgt ggaggaagcc tgaacgggag tgtgcgcggg cagcggaacg ggcagggacg
      781 aaggcgggag tcgacacgat ggatgttgcg ggtttgacac tgtgcgcggc ggcagaaggg
      841 ctgcggaaga agacgtattc ctcagtcgag ctgacacggg ccgtattgga tgcgatccgg
      901 gagaaggatg gcgctctgca cgcgtatctg agtgtggatg aggagggggc gttgcggcag
      961 gccgccgcgg cggatgccgc gcgggcggcg gggcacgaca cggacctgct cgggattccg
     1021 gtggcgatca aggacctgat caatgtcgag ggccagccct gcacatgcgc ctccaatatg
     1081 atgcaggggt atgtggctcc gtacgacgcg acggttgcgg cgcggctgcg ggccgcaggc
     1141 gcggtgtttg caggccgcac caatatggac gaattcgcca tggggtcgtc ttctgagacc
     1201 tcggcgttca aggtcacgcg caacccgcac gatctggaac gggttccggg cggctccagc
     1261 ggcggatcgg ctgcggcggt ggccggcggc gaggccattg cggcgttggg tagcgatacg
     1321 ggcggatcaa tccgccagcc cgccgcattc tgcggttgcg tgggcctcaa accctcgtac
     1381 ggccgcgtgt cgcggtacgg cgtcacggcg tacgcctcat ccctggatca ggtggggccc
     1441 atgacccgga cggttgagga tgcggcggtt ctgttgcaga cgctggcggg tgtggatccg
     1501 catgacggga ccacgttgga cacgccggtg ccggattacc gggcggcact cgtcggcggc
     1561 gtgcgggggt tgcggatcgg cctgccgaag gagtattttg tcgatggcac ggatccagag
     1621 gtgatggcgt gtgtgcgccg ggctgttgat gcgtgcgcga cggcgggggc cgagattgtg
     1681 gaggtgagcc tgccgcacac cgaatatgcg gtcgcggtgt attgcattgt gggcacggcg
     1741 gaggcgtccg ccaatctggc gcgttttgac ggggtgcgct acgggcaccg gacgagccgc
     1801 cccgcatcgg tgttcgacct gtacgcccgt agccgggccg aggggttcgg ccaggaggtc
     1861 aaacggcgca tcattctggg gacgtatgtc ttgagcagcg ggtattacga tgcgtattac
     1921 gggcgcgcgc agaaagtccg gaccctgatc cggcgcgact ttacagaggc gttcgagcgt
     1981 tgcgatgtgc tcttcacgcc ggtggcgccc acgccggcgt accgcatcgg cgagcggtgt
     2041 gaggatcccc tcaccatgta tctgggcgac acgttcacgg tgcccgccag tctggccggg
     2101 atctgcggac tctccgtgcc gtgcggccgg actgcggcag gattaccggt cggactccag
     2161 attctcggtc cggcgttcgg tgagagcgtg gtgctgcggg cagggcaggc ggtcgaacag
     2221 gaggtgcggg catgagtcgt tacctcgtca ccatcggact ggaaacgcat gtgcagctca
     2281 agaccctcac caagatgttc tgcggttgca gtctgacgtt cggcgaggag ccgaacacca
     2341 atgtctgccc ggtttgcctc gggtatccgg gcgcgttgcc ggtgatgaac cgcgaggccg
     2401 tccggttaac ggttctctcc ggcctgatgc tgggatgcga aattaaccgg catagcacgt
     2461 ttgaccggaa gaattacttc tatccggaca tgtccaagga ctatcaaatt tctcagaaca
     2521 cgcacccgct ctgtctgggt ggcggggtga ccatcgagac gccggaggga ccgaaacgga
     2581 tccggatcaa ccatatccac cttgaagagg acgccgcgaa gatcaaccac tacgcgcaat
     2641 tcagcggggt tgatttcaac cgttgcggca cgccgttgat ggaaatcgtt tcggagcccg
     2701 acctcgcgtc gccggatgag gcgatggcct atctcctggc gctccggcaa cagctcgtgt
     2761 atgcgggggt gagcgactgc aatctcgaag aggggaatat gcggtcggat gtgaacatca
     2821 gcatacggcc cgaagggcag acagaatttg gcacgagggt ggaaatcaaa aacatgaaca
     2881 ccttcaaagg gatccacgcg gcgctgaaat atgagatcgc gcggcagctc gccgtggtgc
     2941 gggggaacgg cacgctggtg caggagacgc ggcggtggga tcccgaacag ggggagaccc
     3001 aatcgatgcg cagcaaagag aatgcgcatg actaccgtta tttccctgag ccggatctgg
     3061 ttccggtggc actgacggat gtgcagatcg aggcttggcg ggagcagttg ccggaagcgc
     3121 cggctgcgcg acgcgagcgg atgatggcag agtacggcct cccggcatac gatgcggggg
     3181 ttctggcgga tatccgggag aacgccgatt tctttgaggc tgcggtacgg gccagtaagc
     3241 ccggtatggg gaaggcggtc tccaactggt ttatgaccga ggtcatgcgg ttgctggcgg
     3301 aaacggggca gccggtgggc gcgtgcgcgc tgacgcctgc cgcgcttgcg gaattggtgc
     3361 agctggtgga ggagggcgtg atcaacgggc cgaccgccaa ggagctgctg cccgagctgt
     3421 ttcatgaagg cggcatgccc gccgcgcggg tcgaggcgcg cggtcttgcg caggtgagcg
     3481 atgacgccgc gctccatgcg ttcatcgatc aggtgttccg ggagaacggc aagtcagttg
     3541 aagattatcg ggccggcaaa aaagccgcag cgggttttct ggtcggacag gtgatgaaag
     3601 taagccacgg gaaggcggac cccaaacggg tcggccgact ggtggcagag cggttggcga
     3661 cacggacgtg agcggaccgg tcgtgttacg gggagagcga ttgtgacttc gaatgatgat
     3721 tatgtcgtgc aacttctgcg tgagcagagt tatgtgtccg ccgagcaggt cgaggcggtc
     3781 gcgaactcga tcaagcagga aggggagaca acgctcgata ttctggtcca gaccgggatc
     3841 gtgaccgaag atgacgtgtt gagtctggtc gccgcgcaat tcggcatgga gtacgtgcat
     3901 gtcaaccccg atgccatcga ccctgccgtc accgaggtgg ttccggcgga tgtcgcgcgc
     3961 aagtacggcg tggttccggt cacccagacc gaagacacgg tgaccgtggc gctgagcgac
     4021 cccatggcct acgacacggt ggacagtctg cggtatgtgc ttaaaatgaa cgtcgaggcg
     4081 gttgtggcgc cgcgttccga ggtgcgggcc gcggtcgacc ggttgtatcc ggagtcgttt
     4141 gagagcattt acggggccat gtcggacagc gagatcgatg tggcggagaa ggcgaaaagc
     4201 gacacggggc aggaatccga cgactccgat gccccggtga tccgtctggt cagcatgatc
     4261 atcacggaag cctgcaagat gcgcgcgtcc gatatccatc tggaaccgat ggaacggacg
     4321 ttccgtgtgc ggtaccggat cgacggtgtg ttgcggcaga tggacactcc ccccaagcgg
     4381 ttgcaggcgg cgatcctcag ccgcgtgaag atcatgtcga acatgaagat ctccgagaag
     4441 cgggtgccgc aggacggccg gattcagatc aaagtgcagg ggcgcgacct cgatctgcgc
     4501 gtgtcctccg tgccgaccaa ccacggcgag agcatcgtca tgcgtattct ggacaaatcc
     4561 aacctctcgc tcggtctgcc gcagctcggc ttcctgtcgg atgaccagaa caagttcgaa
     4621 cgtctgatct ccctgccgga tggtgtgctt ctggtgacgg ggcccaccgg gtcgggaaaa
     4681 acaacgaccc tttacgcctg cctcggccag atcaatctgc cggaccggaa aatcatcacg
     4741 gttgaagacc cggtcgaata tcagatgtcc ggcatcaatc aggtgcaggt gaacaaggat
     4801 gtggggttgg atttttccgc cgcgctccgt tcgatcctgc gtcaggcgcc gaacatcgtg
     4861 atgatcggtg agatccgcga tcacgagacc gcggacatcg cgatggaggc ggccctcacg
     4921 ggacaccttg ttttcagcac gctgcacacc aatgacgcgc ccagtgcggt cacgcgtctg
     4981 ctggatatcg gcgtgaagcc gtttctggtg gcgtccgcgt tgcgcgccgc catggcgcag
     5041 cggttggtgc gcgcggtctg cgaaaaatgc cgcgcggaat acaagcccac cgaacgcgaa
     5101 ctcaagatgc tcggtgccat ggccaaaaac gtcacggtgt cgcagatgta caagggcacc
     5161 ggctgttcgg cctgcggaca aacggggtat aaggggcgca agggcatttt tgagattttc
     5221 cagatcgatg acacaatcca acgcctgatc ttcgatcatt cgccggccac ggtgttgcgt
     5281 acccgtgcgc gggagatggg catgcggaca ttgcgcgagg acggcctgct caaggtggcc
     5341 tcgggcatga cgacgctgga ggaggtcttg cgggcaacca tgggagacgc tgactgatga
     5401 gcacagaaat caacatgacc gaattattgc aagccacggt ggatgagggg gcctccgatc
     5461 tgcacatccg cgtcgggatc ccgccgatgc tgcgcattca tggcgcgctg acgccgttgg
     5521 acagtccgcc gttgactcct caggatacgg aatcgctggc ccggatgatc accgccgaag
     5581 acaagttgaa cgagattcaa gagtgcggcg gcgcggactt cgctttgccg ttcggtgacc
     5641 tggctcgttt tcgtgtctcg gttttcaaag agcgggggca tttcggcatc gtgttgcgcc
     5701 agatcccgag taaactgctc acgctggagc agatcgggtt gccggagcag gtcaaagagt
     5761 tgcttttccg gccgcgcggg ctgattctgg tgaccggccc caccggttcc ggcaaaacca
     5821 ccacgctggc cagcatgatc gacatcatca accgcgagcg cgacgtccac atcatcacgg
     5881 tggaggatcc catcgaatat taccacccgc ataaaaaatc ggtggtgacc cagcgcgaga
     5941 tcgggcatga tgtgacgtca ttcgccgaag cgatccgccg tgcgctgcgc caggatccgg
     6001 atgtgatcct cgtgggggaa atgcgcgatc tggaaaccat gcaggccgcg atcacggcgg
     6061 cggaaaccgg ccatctggtg ttcgccacgc tgcataccac cggtgcggcc gccacggtgg
     6121 accgcatcgt ggacgccttc cccaccgatc agcaggaaca ggtccgtacg cagatgtccg
     6181 tggggctcgt ggctgtgatt tcgcagttgc ttctggcgcg tgtggacaag cccggccgtg
     6241 tggcggcgtt cgaaatcatg atttcgaccc cgtccatccg ttcccggatt cgcgataaca
     6301 agacattcca aatctcgtcc gatatccaga ccggagccaa attcggcatg atctcgctcg
     6361 actcgcatct gatggacctc tatctgggcg ggctgatctc atacgatgat ctcgtcacca
     6421 aatcgcagga tccggatacg ctggtccaga aactgaaaga cgaacttcag tcaaggagga
     6481 actgatggct gtgacttcga catccgttgc gccgccctat catccgttga tcgacctgct
     6541 ggtcagcaac ggcatcattg accagcagca ggcggaagag ttgcgcgacg agcagaaaaa
     6601 ctcgggcaag ccctttcgca ccctgctggt tgacgccgga tacgtgagcg aagacgatct
     6661 gctgggtatg atggccgcat atcagggttg tgacgtgata gacctcgaga tgatgtcgct
     6721 cgatacggaa gtgatccaga gtatccctgc cagtgtggcc cgcatgtaca atgtcctgcc
     6781 ggtgtctgcc actgccggaa ccatcacgct ggccacaagc gatctggttg atccgcacgt
     6841 tacggacgaa ttgatgttcg tgctgacccg cgacgtgcag ttcgtcatgg cgcgcgaaaa
     6901 tgatgtgcgc acgcgtgtcg ccgaatacta cggcgacgag agcgcgactg tcgcggacat
     6961 gctcaacagc ctcgagtcgg atctgaagct ggacacctcg ctggatgtgg atgatacgga
     7021 tgacaagagt ctggaatccg ccgcgtccgc cgcgccggtc atccggttcg tgaacctcgt
     7081 gctctatcag gcggtcacgg accgggcctc cgacatccat ttcgaaccgt ttgaaaccga
     7141 tttcaagatc cggtaccgcg tggatggcgc cctgtatgag atgtcgccgc cgccccgccg
     7201 tctggctctg ccgatcatct cgcgcattaa ggtcatgtcc ggcctgaaca tcgcggaacg
     7261 gcgtgtgccg caggacgggc gcatcgcgct gaccgtggcc ggccgttcgg ttgatctgcg
     7321 tgtctcctgc ctgccgaccg cgcatgggga aagcgttgtg ctccgcgtac tggaccgcag
     7381 cgtggtttcg cttgatctcg agaacgtcgg tctgccggaa gatgtctatg aggaactgac
     7441 gctggacatc gagaaaccca acgggatcat catcgtcacg gggccgaccg gttccgggaa
     7501 aaccacgacg ctgtactcct gtctgcgccg gatcaacacg attgatcaga agctgctgac
     7561 cgccgaagaa ccggtcgaat acgacatgga cggcattgtt caggttcaga tcaatccgag
     7621 tgcggggaac acctttccaa gggtgttgcg cgcctttctg cgtcaagacc ccgacatcat
     7681 gatgatcggc gaaatccgcg acctcgagac cgccgagatc gcggtgcagg cctccctcac
     7741 cggccacctg gtgttcagca cactgcacac caatgatgcg gccggtgcgg tcacgcgcct
     7801 gattgacatg aatgtggcgc cgtacctcat cgcttccacc ttggaggcgg tgcttgggca
     7861 gcgtctggtc cgcaccatct gcctgaactg caaggagccc tacacgccgg atgatgagac
     7921 cttgagccgt atggacctga aacgcgccgc ggtcggggat cggccttttt attacggccg
     7981 gggatgttcc aagtgcaacg gaaccggata caaggggcgg aaaggggtgt tcgaatatct
     8041 gcgggtgagc gatcccatcc gcgagttgat caacgaacgt aggccgaccc tgttcatccg
     8101 ggaacgcgcc cgggagttgg gtatgcggac catgcgggaa gacgcgatcc gaaatgtgct
     8161 tgacgggtat accacggtag atgaggtgtt gcggtacacg taggcgggag cgccgcttgc
     8221 ggttgattcc ggtgagggga gggacttgtg gaaaatgtgg tgatcattgg gagtggaccc
     8281 gccggtctga cggcggcgat ttatgcggcg cgcgccaacc tgaatccggt gctgatcgaa
     8341 ggcatgcagc ccggcggaca gttgacccag acgaccgacg tcgagaattt cccgggattc
     8401 gagaatccga tcgatggaac cacgctggtc atgaccatgc gcaagcaggc ggaacgcctc
     8461 ggcgtacgct gtttggccga cgaagtgtcg gcatgtgcgc tggctgcgaa agagaagacc
     8521 gtcacgctga tgctgggggg ggaactgcaa acgcgtgccg tgatccttgc caccggtgcc
     8581 agcgcgcggt atttggggct cgagtccgag caacggctga ttggcaaagg cgtctcggcc
     8641 tgcgccacct gcgacgggtc atttttcaag ggacagccgg tggcggtcat cggcgggggg
     8701 gatacggcga tggaggacgc gctgtatctc tcacgcatag ccgaacgggt gacagtcgtc
     8761 catcgccgcg acacgttccg cgcctcgaaa atcatggcgg gacgggtctt ggcttcgccg
     8821 cagatcgagg tggcctggaa ttcggtggtg gatgaggtgt tgggtgcgga ggaggtcacg
     8881 ggactgcgtc tgcggcacgt tcacaccgga gccctcacgg agattccggt gaacggagtg
     8941 tttgtcgcga tcggccatac gcccaacact gcgctcttcg ccgggcaggt tgaactggat
     9001 ccggagggat atgtggtcac gcgtaacacg cgcacctccc tgccgggcgt gttcgcggca
     9061 ggggatgtgc aggaccgtca ctacaaacag gcggtcactg ccgcgggatc ggggtgtatg
     9121 gccgcattgg aagcggagcg gtttttggct tcaggcgaat aggatatggt tgaatcattt
     9181 gactttgata aagagtattc gtttgccgaa gcctacggaa ggctttaccg ttatgcgcgg
     9241 aaatatcgct tccgcctgct ggccggtctg ctggccggat cggtcacggc gggttccatg
     9301 ctgcctattt atcaggtggt gcagccggtc attcggaaca tgtattcggt tgaggagatc
     9361 gtgcccgccg cgcggcatcg cctgccaatg gcggatctgg ttgaacccgg ccgcctccat
     9421 ccagcgccgc accggcggca cgatctcctg cggatacacc gccacgccgg tgggcaccgt
     9481 gacaggcaac ggtttgcgct tgccggggcc gaagctctcc cagtagagcc gggccgacga
     9541 cgcggcgct
//
LOCUS       DAOSRB010000112         9408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_7036_length_9408_cov_5.711964, whole genome shotgun sequence.
ACCESSION   DAOSRB010000112 DAOSRB010000000
VERSION     DAOSRB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7036_length_9408_cov_5.711964"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLZ74_07205"
     CDS             complement(<1..407)
                     /locus_tag="PLZ74_07205"
                     /inference="COORDINATES: protein motif:HMM:NF037184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOR98145.1"
                     /translation="MKPWMLQGTEAFSSFRLKALTGRVQACLDRPQAVRVEAHFVYLL
                     DCARKPDRLTLDRACTLLGATEGPEPQGGFFVTPRKGTVSPWSSRATDLFRNCGLGGI
                     SRVERGVHIRVFAQETELSLAALRPALNMLHDR"
     gene            543..788
                     /gene="rpoZ"
                     /locus_tag="PLZ74_07210"
     CDS             543..788
                     /gene="rpoZ"
                     /locus_tag="PLZ74_07210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOR98146.1"
                     /translation="MNVELIEKARVRIPSVPVLVNLVSKRVRQLNMGERPYVKPLSAD
                     EDKCDIALREIAEGLLISEVDFSAIARAEFAHSRWSE"
     gene            819..1301
                     /gene="smpB"
                     /locus_tag="PLZ74_07215"
     CDS             819..1301
                     /gene="smpB"
                     /locus_tag="PLZ74_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOR98147.1"
                     /translation="MSTGEKKRKGGNLAVNRRAHHDYFVLEKIEAGIELVGTEVKVVR
                     NGEAGLIGAYAQVKNGQVYLNQLRIPPYAFGNRFNHDALRTRRLLLHKREIRKLQAVT
                     EQKGLSLIPLRMYLTPKGLVKVELGICRGKAQEDKRETVRRRDADREARRAMAKHYKG
                     "
     gene            1327..1575
                     /gene="tatA"
                     /locus_tag="PLZ74_07220"
     CDS             1327..1575
                     /gene="tatA"
                     /locus_tag="PLZ74_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOR98148.1"
                     /translation="MTQPVGFLGMNVGPTELLVVLAIILVLFGGKKLPDLARSLGRSL
                     GEFKKGKEEGEKLANEKVREIVDDIKKEEPEKVEGPKA"
     gene            complement(1687..4014)
                     /locus_tag="PLZ74_07225"
     CDS             complement(1687..4014)
                     /locus_tag="PLZ74_07225"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOR98149.1"
                     /translation="MMMRFRPIFLSLLCVSVFSGVRADPAVVPIGMAEGAAGAVVPLP
                     QGLPVAFPGEVNVPLASTVLEPWLKADALHLAVAWPEGKPERVGALVWLKDRDAFWHQ
                     YLIPQSGEGKETNLWVIPFRPGAAGWQTPGHTLAWHHRVRMSPQSVGVRLFGDSVFTG
                     KCVLVSAFLTPQPSASPPEIVKVRPLTRSARAYALYEARFDLPDRYDNPFDPGQIDAG
                     AVVITPAGQTNAIHAFYYQPHYRLEDELGQPVEPDGQPEWRIRYCPREAGEYRVSLIA
                     RDAHGEVTLTNALRFHAAPADPEAMRFIRVSQKDPRFFELDDGRLFYPIGHNVRSATD
                     SRMDDKFPWVFRQDEGPTAYRRYFSRMQANGENIAEVWMSAWSLGLEWTEGVSGYHGA
                     NDYHMGNAWELDRVIDLAHEHNLYINLVLNYHGRISTWCDPEWHLHPYNKATPGGWLT
                     RPLEFFSDERALEMQRRFCRYTQARWGWAPAIFGYELCSEINLTGHETHHKTHFDPAV
                     VAWCRALGGYLKRIDVYGHLVSAHVSNDYTFLNPAISNLTEMDFNPLDAYHHGQPERI
                     IPLVAATADAGAAYNKPILITEFGGSPMAAGREHLLIEQHAALWAGACVPMSGVPMFW
                     WWQVIDENDLYGRYAAIQTFMTGIDPRNALAKQVKPALGVSENGDPLLLKRYGALGTA
                     APETLRCYVHPVRFARGDAKLPVAAHLTVTVSGMTPGIFRVEVCETSTGNPVRRFDVR
                     TADGTLTVPLPPFSPDCAFKAHLLTPLAAKPAAAP"
     gene            complement(4096..4263)
                     /locus_tag="PLZ74_07230"
     CDS             complement(4096..4263)
                     /locus_tag="PLZ74_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98150.1"
                     /translation="MGETTGLRDTETGSPDSFGRYIMSKNHDAKKEAKKKPTKTLKEK
                     RLEKKAKKTAQ"
     gene            4417..5250
                     /gene="recO"
                     /locus_tag="PLZ74_07235"
     CDS             4417..5250
                     /gene="recO"
                     /locus_tag="PLZ74_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOR98151.1"
                     /translation="MIIRTPALALQIRPWSQTSQMVTWLTPGHGKIVTAVKGACRPKS
                     AFLGQYDLFYTCDLLFYRRDRGGVHAIRECSPVTLRSALRQNWRHATAAAYLADLTAQ
                     TAVGHQETTALFELLTCALDHLTTARCVPRELIVWYETHLLRLLGLLPDFTTCPLCHP
                     PERRWMRFSLPSGRFVCPHLAQHHPDDAVITLHRGVPALFHRFSRLGTPPADNMKHRP
                     TVPPPSPPPENLLLGLCRFLGIFIPFHLDVPSVVRRVTFEMIDTQHICGTCAALREIG
                     S"
     gene            5247..6350
                     /locus_tag="PLZ74_07240"
     CDS             5247..6350
                     /locus_tag="PLZ74_07240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR98152.1"
                     /translation="MMKCSPILLCTALTTTLFLTGGCKKEATGQPAANMDAAVAAAAQ
                     TPETSTDPNEVVASVNATRYLRKDMDQTVSALLKAQNIPEAQLEDARKHFEQRVVYGF
                     IMKTLLLDEAKKQTVTVTEEDRKAQIDKIAEALKPQNKTTEQYFKDSPLGEKVARAEF
                     EDGLVIDKLLRKTVLDSIVIDEEEVKKTLAEIEARNAEIAATNKNLIAINAERKAKIQ
                     ELKKQLDNGADFAELAKAHSECPSKEQGGDLGPFTAGQMVKPFEDAAFAQEINKVGDI
                     VETSFGYHLIKVTAKSPATEAKGDTPAKPASVTASHILIKTESPQELQEVPTSEQIQN
                     HLKQQKSRAAVEKYIEGLKAAAKIETIYKEIPL"
     gene            6506..7660
                     /locus_tag="PLZ74_07245"
     CDS             6506..7660
                     /locus_tag="PLZ74_07245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR98153.1"
                     /translation="MSVKVLMLDLVSEWGGAQHSLYDACVSLSRLGVEPVAAVPQGPL
                     FDRLTTAGITVFPVSPLRAHRRGFALFTTAAKLLRAPATVSQILKVVKPDIIHCNSLQ
                     AFLAVRGHHAKTPLIWHVRDLKMPVALARDAAKKAARLFAVSEAVDEYLVEILPQKHL
                     GRIRVVRNGIDLQRFGQTCRVEARSRFGLPPDAPIVGMIAHLAPWKRHDAFIRTAARI
                     RARQPDAHFVSVGRDLFGENTRWRTQLNAEVEKERLSECFHWVTDLDQAETILPAFDV
                     LVHPALREPFGRVICEAMASGVPVVAAESGGPATLIRQQVTGVLVRDGDEEGMADAAL
                     ALLADRVRASQIADAARAFVQEHHAIEQTAEQLLKEYHSLLASETGRYET"
     gene            complement(7670..9016)
                     /locus_tag="PLZ74_07250"
     CDS             complement(7670..9016)
                     /locus_tag="PLZ74_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98154.1"
                     /translation="MIALARKIKARLGRLWWYTAILFLVLRACDLCNAFIGLWLVPRY
                     VPQDELGAVLPLTQVGGVLGLPLAILLVPFTKFLNTYATRGEYGKVKRLLRDVFVLSL
                     LLFVGTLCFARFILPLVFERMRVGAGSLGLLIVASGVLSVLSPLFINALQALQQFRMV
                     AVINLIAAPIRLLAMWIAMPFRALSGYFVGQSAPSLFLIGASLFGLRRHLGREVRSEP
                     YWQKDWHSILRYTLPVAMMTVANAVQTAVESFVIRHRLSELDSAGYYVISRFAEIGAY
                     VGLTFMFVLFPLAAERHERGKRSDRYLWHAIGGTLVSGLILTGLFALFGEWLLRLNPV
                     WSDYAGYDHAMVVLTLVVVARTAASCFTSHEMACGRFGFVCGFSLIAGIECAVLYGLT
                     GVSFFYGILPTSWVDGLASLQAGRLEFILNVMLASAVALCAYVGLHEFRRRRQTTG"
BASE COUNT         2068 a         3010 c         2651 g         1679 t
ORIGIN      
        1 atgcggtcat gcagcatgtt caacgcggga cgcaacgccg cgagcgacag ttcggtctcc
       61 tgcgcaaaga cccggatatg cacgccgcgc tccacgcggc tgattccccc taacccgcaa
      121 ttgcggaaga gatcggtcgc tctggatgac cagggcgaga ccgtcccctt gcgcggcgtc
      181 acaaaaaaac cgccctgcgg ctccggcccc tctgtcgcgc ccagcagcgt acaggcccgg
      241 tcaagtgtca gccgatccgg tttccgggca caatccagca gatagacgaa atgtgcctcg
      301 acccgcacag cctgcggacg gtcgagacat gcttgaaccc tcccggtcag cgccttcaga
      361 cggaaagaag aaaaagcctc agtaccctgc aacatccatg gtttcatccg tacgttcctt
      421 tatcctgtcg cgatttgcgg ggcagaagcg tatcaaagcg gcccggcgca ttcaagcaaa
      481 ctgcggattg accatcacgt caggagcctc tatactaccg gcttaatcct tggaggcatt
      541 ttgtgaacgt tgaactgatt gaaaaagcga gggtccggat tcccagcgtt ccggtccttg
      601 tgaatctggt gtccaagcgc gtgcgccagc tcaatatggg cgagcggccc tacgtcaagc
      661 cgctttccgc ggacgaggac aagtgcgaca tcgccctgcg ggagatcgcg gaaggcctgt
      721 tgatctcgga ggtcgatttc agcgccatcg cccgtgcaga gttcgcgcat tcgcgttggt
      781 cggagtaacc cttccgcgga acggcggcgc aggtcagcat gagtaccggc gagaaaaagc
      841 ggaaaggcgg caatctggct gtcaaccgca gggcacacca cgactacttt gttcttgaaa
      901 aaattgaggc cgggatcgaa ttggttggca cggaagtcaa agtcgtgcgc aacggcgagg
      961 cgggactgat tggcgcatat gcccaagtaa agaacgggca ggtttatctc aatcagctac
     1021 gtatccctcc ctatgcgttc ggcaaccgtt tcaaccatga cgccttgcgt acccggagat
     1081 tgttgttgca caaacgggag atccgcaaac ttcaggctgt gaccgaacaa aaaggacttt
     1141 cgcttattcc cttgcggatg tacctcacgc cgaaagggct ggtcaaggtc gaactgggga
     1201 tttgccgcgg caaagcgcag gaggacaagc gcgaaaccgt cagacggcgt gatgcggaca
     1261 gggaagcccg ccgcgcgatg gcgaaacact acaaaggtta agaggttttc aaacaaggag
     1321 gagcatatga cacaacccgt aggttttctg gggatgaatg tcgggccgac cgaactgctc
     1381 gtcgtgttgg cgatcattct ggtgctgttc ggcgggaaaa aacttcctga cctcgcgcgc
     1441 tcgctgggcc gcagcctggg cgagttcaag aagggcaagg aagagggcga gaaactggcc
     1501 aacgagaagg tcagggaaat cgtcgacgac atcaagaaag aggaaccgga aaaggtcgaa
     1561 ggccccaagg cctgacccac cgcttttccc tactcccgac tcctgtctcg cgtctcttgt
     1621 ctcctgtctg ccgaaaccgt ttcgggcttc gggacgggat gccttcccga ggcgtatcgt
     1681 cccgtctcag ggagcggccg cgggtttggc cgccagcggc gtcagcagat gcgccttaaa
     1741 cgcgcagtcc ggcgagaagg gcggcagcgg aaccgtcagc gtaccgtccg cagtccggac
     1801 atcaaagcgc cgcaccggat tgccggtgga ggtctcgcag acttccacgc gaaagattcc
     1861 cggcgtcatg ccggaaaccg taacggtcag gtgcgcggca accggcaact tggcgtcgcc
     1921 gcgcgcgaac cgcaccgggt gcacatagca gcggagcgtt tcgggagcgg cggtgcccaa
     1981 cgcgccatag cgtttgagaa gaagagggtc cccgttttcc gacaccccca gtgccggctt
     2041 cacctgcttg gcaagggcat tgcggggatc gataccggtc atgaaggttt ggatcgccgc
     2101 ataccgtccg tacagatcgt tctcatcgat cacctgccac caccagaaca tcggcacacc
     2161 ggacatcggc acacaggcgc cggcccacag cgcggcatgc tgttcaatca gaaggtgctc
     2221 gcgtcccgcg gccataggcg acccgccgaa ctcggtgatg aggatcggct tgttgtacgc
     2281 cgcgccggcg tcagcggtcg ccgccaccag cgggatgatg cgctcgggct gcccgtgatg
     2341 ataggcgtcc aggggattga aatccatctc cgtcagatta gagatggcag ggttgagaaa
     2401 cgtgtaatcg ttggacacat gcgcggaaac cagatggcca tagacatcga tgcgcttgag
     2461 atatccgccg agggcacggc accaggcgac gaccgccggg tcgaaatgcg ttttatgatg
     2521 ggtttcgtgc cccgtgaggt tgatttcact gcacaattcg tacccgaaaa tcgcgggcgc
     2581 ccacccccag cgcgcctgcg tgtagcgaca gaaacgacgc tgcatctcca gcgcccgttc
     2641 gtcgctgaaa aattcgagcg gccgggtgag ccaacctccg ggagtcgcct tgttgtaagg
     2701 atgcagatgc cattccggat cgcaccatgt gctgatacgc ccgtggtaat tcagcaccag
     2761 gttgatgtac aggttgtgct cgtgggcgag atcgatcaca cggtccagtt cccatgcgtt
     2821 gcccatgtgg taatcgttcg ccccgtgata cccgctcacg ccttcggtcc actccaatcc
     2881 cagcgaccac gcgctcatcc agacttcggc gatgttctcg ccgttcgcct gcatgcggct
     2941 gaaataacgt cgataggcgg tcgggccctc atcctgccgg aacacccagg ggaacttatc
     3001 atccatccgc gaatccgttg ccgagcgcac gttgtggccg atcggataaa agagccgtcc
     3061 gtcatccaac tcgaaaaagc gcgggtcttt ttgcgagacc cggataaagc gcatcgcttc
     3121 cggatcagcc ggagcggcat ggaaccggag ggcgttcgtg agcgtcacct caccgtgcgc
     3181 atcgcgcgcg atcagagaga cccggtattc acccgcttcg cgcggacagt accggatgcg
     3241 ccattcgggc tggccgtccg gctcaaccgg ctggccgagt tcgtcctcca gccggtaatg
     3301 cggctgatag tagaaggcat ggatcgcatt cgtctggccc gcgggtgtga tcacgaccgc
     3361 acccgcgtca atctggccgg gatcgaaggg gttgtcatag cggtcgggca gatcaaaccg
     3421 cgcttcgtac agggcatagg cgcgcgcgga acgggtcagc ggccgcacct tcacaatctc
     3481 cggcggcgac gcggacggct gcggcgtgag gaatgccgac accagaacgc acttgccggt
     3541 aaagaccgag tctccgaaaa ggcggacccc cacgctctgc ggactcatcc ggacacgatg
     3601 gtgccaagcc agcgtgtgcc ccggcgtctg ccatcccgcg gccccgggcc ggaacggaat
     3661 gacccacagg ttggtctcct tcccctcccc gctttgcggg atgagatact ggtgccagaa
     3721 cgcgtcacgg tctttgagcc aaaccaacgc cccgacccgc tccggtttcc cttcgggcca
     3781 agccacggcc aggtgcagtg catcggcctt cagccagggt tcaagaaccg tcgacgccaa
     3841 cggcacattc acttcaccgg gaaaagccac gggcaagccc tgcggcaacg ggacaaccgc
     3901 accggccgct ccctccgcca tcccgatcgg gacgacggca ggatcggcgc gcactccgga
     3961 aaaaacagac acgcagagca gagagagaaa aatcgggcgg aaacgcatca tcatgccgga
     4021 ttaagaagca ccccccatgc cagaaaacaa ggggggggaa atgcgctcca gacaaaaccg
     4081 gagcgcgcga aaaggtcact gtgccgtttt cttggctttt ttctccagac gcttctcttt
     4141 cagcgtcttg gtcggtttct ttttcgcttc ttttttcgca tcgtgatttt tggacatgat
     4201 gtacctcccg aacgaatcgg gcgaaccggt ttccgtatcg cggagcccgg tcgtctcgcc
     4261 caagttggcc gcaccttaac aggttgcctc cccccttgtc aaccgctcgc cgtcaattct
     4321 tcttttaccg ggaaaacccg acgggtgccc caccctgcgg gttacggctt gtctaccgcg
     4381 ccaaaagagg cgatactccc cccatatccg acacgtatga ttatccggac accagccctt
     4441 gccctgcaaa tccgcccttg gtcccaaacc tcgcagatgg tgacctggct gacaccgggt
     4501 cacggcaaga tcgtgaccgc cgtgaaaggg gcctgccgcc ccaagagcgc ctttctggga
     4561 cagtatgacc tcttttacac ctgcgacctg cttttctatc gcagagaccg cgggggcgtg
     4621 catgccatcc gcgaatgttc gcccgtcacc ctgcgaagcg cgttacgcca gaattggcgc
     4681 catgcgacgg ccgcggcgta ccttgccgat ctgaccgccc aaacggcagt cggccatcag
     4741 gagaccacgg cgctgttcga actgctgact tgcgcacttg accacctcac gacggcgcga
     4801 tgtgtcccgc gcgagctgat cgtttggtac gagacgcacc tgctccgtct gttgggactg
     4861 ttgccggact tcacgacctg ccccctctgc cacccccccg agcggcgctg gatgcgattc
     4921 tccctcccct ccggacgctt tgtctgcccg cacctcgccc agcaccatcc ggacgatgcc
     4981 gtcatcacgc tgcaccgggg ggtccccgcc ctcttccatc gcttcagccg actcgggacc
     5041 ccacccgccg acaacatgaa gcatcggccg accgtgcccc ccccctcccc tccccctgaa
     5101 aatcttctcc ttggcttatg ccgatttctt ggtatcttta tcccgtttca cctcgatgtg
     5161 ccttcggttg tccggcgtgt cacgttcgag atgatcgaca cacaacacat atgcggtaca
     5221 tgcgccgcat tacgggagat cggttcatga tgaagtgctc acctatacta ctctgcaccg
     5281 cgctgaccac cacactgttc ttgaccggcg gatgcaaaaa agaggcgacc ggacaaccgg
     5341 ccgcgaacat ggacgcggca gttgcggccg ccgcgcaaac gcctgaaacc tccacggatc
     5401 caaacgaggt ggtcgcttca gtcaatgcga cccgctatct gcgcaaagac atggaccaga
     5461 ccgtctctgc cctcctgaag gcgcagaaca tccccgaggc ccaactggag gatgcgcgca
     5521 aacatttcga gcaacgcgtc gtctacgggt tcatcatgaa aaccctgctg ctggatgagg
     5581 ccaaaaagca aacggtcacg gtgacagagg aagatcgtaa agcgcagatc gacaagatcg
     5641 ccgaggcgct caaaccccag aataaaacga cggaacagta tttcaaagac tccccgctgg
     5701 gggaaaaggt tgcccgcgcc gaattcgagg acggacttgt catcgacaag ctgctgagga
     5761 aaaccgttct ggacagtatc gtcatcgacg aggaagaggt gaagaaaacc ctcgcggaga
     5821 tcgaagcgag aaatgccgaa atcgcggcga ccaacaaaaa cctcatcgcc atcaatgccg
     5881 aacgcaaagc caaaattcag gaactcaaaa aacagttgga caacggtgcg gactttgcgg
     5941 aactcgccaa agcccattcc gagtgcccga gtaaggagca gggcggcgat ttgggtccct
     6001 tcaccgcagg acagatggtc aagccgtttg aagatgccgc attcgcgcag gaaatcaaca
     6061 aagtcgggga tatcgtggag acctcgttcg ggtaccacct gatcaaggtg acagccaaat
     6121 cgccggccac ggaggccaaa ggcgacacac ccgccaaacc ggcttcggtg accgcatccc
     6181 acattctgat caagacggaa agcccgcaag agctgcaaga agttcccacg tccgaacaga
     6241 tccagaacca tctgaaacag cagaaatcac gcgcagcagt ggaaaaatac atcgaaggcc
     6301 tcaaagccgc ggccaaaatc gagacgattt acaaagaaat tccgctgtaa gcgaaacccg
     6361 cgcttgcggc gtgcggcccg aataatcggg ccgcacgctt tttttgcctc ccgcccacgc
     6421 catccatgcg tccttaccca aaggcgaaac ggaaaaaaga tcgcgcggct tgattccctg
     6481 tctgaatctg ataagatatt ttcttatgtc tgtaaaggtt ctgatgcttg acttggtgtc
     6541 agaatggggc ggtgcccaac acagcctcta tgacgcctgt gtttcgctct cccgtttggg
     6601 cgttgagcct gttgccgccg tcccgcaagg gccgttgttt gaccgtctga ccactgcggg
     6661 catcaccgtg ttcccggtct cgcccctgcg ggcgcaccgt cggggcttcg cgctgttcac
     6721 cacagcagcc aaactgctgc gcgcgcccgc aacggtttcc cagatactca aagtcgtcaa
     6781 acccgacatc atccattgca acagtttgca ggcctttctc gcggtccgcg gccatcacgc
     6841 aaagaccccg ctcatctggc atgtccgcga cctgaagatg ccggtcgccc tggcgcggga
     6901 cgcggcaaaa aaagccgcgc gcctttttgc cgtgtccgag gcggtggacg aataccttgt
     6961 tgagatcctg cctcaaaagc accttggccg tatccgcgtg gtgcgcaacg ggatcgatct
     7021 ccagcgtttc ggccagacct gccgggtcga ggcccgcagc cgtttcggac ttccgccgga
     7081 tgcgccgata gtcggcatga tcgcgcatct cgccccctgg aaacgccatg acgcgttcat
     7141 ccggacggcc gcgcgtatcc gcgcgcgaca accggacgcg catttcgttt cggtgggtcg
     7201 ggatctgttc ggggaaaata cccgctggcg cactcaattg aatgcggagg ttgagaaaga
     7261 gcgtctttcc gaatgttttc actgggtcac agacctcgat caggctgaaa cgattctgcc
     7321 agcgtttgac gttctggttc atcccgcgtt gcgcgaaccc ttcggccgcg tgatctgtga
     7381 ggccatggcc agcggcgtcc cggtcgtcgc cgccgaatcc ggagggccgg ccaccctgat
     7441 ccggcagcag gtcaccggtg tgctggtccg ggacggggat gaggagggca tggcggatgc
     7501 ggcgctcgcg ctactggcgg atcgcgtgcg cgccagccaa atcgcggacg cggcgcgcgc
     7561 attcgtgcag gaacatcacg cgatcgaaca gacagcggaa caactcctca aagagtacca
     7621 ttcgctactg gcctccgaaa ccggcaggta cgagacctga tgaaaccgct caccccgtcg
     7681 tttgtctgcg ccgcctaaat tcatgcaggc cgacgtaggc acacagagcc acggcactcg
     7741 ccagcatgac attcaggata aactccaaac ggccggcctg gagtgaagcc aatccatcca
     7801 cccatgacgt gggcagaatc ccgtaaaaaa aggagactcc ggtcaacccg tataacaccg
     7861 cgcactcgat gcccgcgatc agcgagaacc cgcagacaaa accgaaccgg ccgcacgcca
     7921 tctcatggct cgtgaaacaa ctggcggcgg tacgcgccac aacgaccagc gtcaacacca
     7981 ccatcgcgtg gtcgtaaccc gcataatcgc tccaaaccgg attcagccgc agcaaccatt
     8041 ccccgaacag ggcgaacagt cccgtcagaa tcaacccgga cacaagcgta cccccgatcg
     8101 catgccaaag atagcggtcg gagcgtttcc cccgctcatg gcgttcggca gccagcggga
     8161 acaggacaaa catgaacgtc agcccgacat acgccccgat ctccgcaaaa cgggaaatga
     8221 cgtaataccc cgcagaatcc agttcggaca accggtggcg gatcacaaaa gactccaccg
     8281 cggtctgcac ggcattcgcg actgtcatca tcgcaaccgg gagggtgtac cggagtatcg
     8341 agtgccagtc tttctgccag tacggttcgg aacgcacctc cctgcccaag tgcctccgca
     8401 gaccgaacag cgaggccccg atcagaaaca gcgagggcgc cgactgcccg acaaagtacc
     8461 ccgaaagggc gcggaacggc atggcgatcc acatggccag caagcgaatc ggagccgcga
     8521 tcaggttgat gaccgccacc atccggaact gctgcagcgc ctgaagcgca tttatgaata
     8581 agggcgacag cacgctgaga accccggaag ccacaatcag caggcccagg gatcccgccc
     8641 cgacacgcat gcgctcaaac acgaggggca ggatgaaccg ggcaaaacac aacgtcccga
     8701 caaacagcag caacgacagg acgaacacat cccgcaacag ccgcttcacc tttccgtatt
     8761 ccccgcgtgt cgcataggtg ttcaggaact tcgtgaacgg caccagcaga atcgccagcg
     8821 gcaaacccaa cacgcctccc acctgcgtca gcggcagaac cgcccccaac tcatcctgag
     8881 gcacataccg gggaaccagc cataagccga tgaaagcgtt gcataaatca cacgccctca
     8941 aaaccagaaa cagaatcgcc gtgtaccacc acaaccgacc cagtctcgct ttgattttcc
     9001 tggctaacgc gatcatattc cccgtatccg ctccacccta cgcaaccggt aagaacgccc
     9061 gcttcaggaa ggtcgtctcc ccccctcccg cctcggagtt cctgcgatgc gcctgtttta
     9121 tatcacaagt gcggcagacc cggaacagaa aaccgctccg agcgtgatca agacgcgctc
     9181 ctgttcccct gggcggtgag gtttcgtttc atgctgcgga cgcctcaagg aggcgcctca
     9241 gtgccccacc acgacctgcc gccgacatgt tgccttttcc ggcttgcctc aaagcgtgaa
     9301 tcgggttacg ctctccctcg gtcacgcgtt cgtaagacac ttttgtctcc ttcctgcttt
     9361 ttcacatgaa ggaagtgtac ttcaacgcgt ggccgctttc aaaacttt
//
LOCUS       DAOSRB010000113         9393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_7048_length_9393_cov_7.451274, whole genome shotgun sequence.
ACCESSION   DAOSRB010000113 DAOSRB010000000
VERSION     DAOSRB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7048_length_9393_cov_7.451274"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1344
                     /locus_tag="PLZ74_07255"
     CDS             196..1344
                     /locus_tag="PLZ74_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR98155.1"
                     /translation="MRTMLFACVAGAVLTTRAQVEAVWLTHRSATPEKTVVSWKSVTP
                     GPSRVAYGADAACGQTASVAGERSVHHVEIDTPQRGATVFYRVETGDQKSEVHSFQTC
                     PEDGVRVAIVGDWGYANRPDLTALKADKPNILMTVGDNVSDVINRKQPGDITNIRPYI
                     GLLRSEAAFFASTLFMPILGNHDKQVGPRVNKRPGSSKETYDIDATAYLSVFALPEPG
                     WRWAFTIPQADVTFLALDMHHLSDFGTTFQTSHDHRQGSEQFAWYDEQTRNLKTGFAV
                     TLLNADSRCRNREKGAWHQMFSRGTIFVTGYGYYAERAVERNGFPYYNTCVARPGDVY
                     RDPLAVVCERVSSYMLLTFDKAKGQMTVDLKRLDGSLIDRQVYHAKPR"
     gene            1430..2764
                     /locus_tag="PLZ74_07260"
     CDS             1430..2764
                     /locus_tag="PLZ74_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98156.1"
                     /translation="MTWTRRDFLSASAGAALAWRSVAAQQPVTAAREGWLEAFRAAGF
                     DPAAAGNSVFVVCGDVHFPQYGQHLPSQIEAWNAMRPAPQFVALLGDNVCNVSSSFGH
                     TPDAKGLERARKELEGLRAETAKLRKEIPLKLVIGNHDTLPRETDAAFFRKVFPACRP
                     YEAFEVSGICFQIWNGAHDGAIDPAQRVWIGKQCAGLPRDKSVVILVHQPSLGMTERE
                     RGIPAMVRAHFSEHTGPLWLLAGHVHSNSIREFALPRTRITQATHVKSVDGYWIYGMR
                     DGRITARVYCPVAGACKAQAMPDPARPARPIPLPFEGRDDVVWRLLIGEDATAIKTAF
                     VRGKGGDCGTWWFYVNELVYKLPLASRGRGATRFAVLAALSKHRKTGELPKVYASGSD
                     GDWIETPLLESRGAEHLFAIPEGLRKSRELYVRIQSYGYGADTCVGGFALCR"
     gene            2814..4754
                     /locus_tag="PLZ74_07265"
     CDS             2814..4754
                     /locus_tag="PLZ74_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR98157.1"
                     /translation="MNTIKRKFLCRRGMMLLVVLGTGAAVQAATVLVEAEGFANAGGW
                     VIDQQSMDQMGSPYRMAHGLGVPVADAETSVQVPEAGRYRVWVRTRDWVATWGAPGAP
                     GAFRVLINGRALDPVFGTQRAEWHWQPGGEIELPVGPVTIALRDLTGFNGRCDAILLT
                     TDVGLKPPDGGGELAALRRQWLGLPDAPEDAGEFDLVVVGGGMAGCCAAVSAARLGCR
                     VALIQDRPVLGGNNSSEVRVGLSGQIRQQPYPRLGELVDEIGPVGHWNLYDAKRAPEL
                     PRSREVLEIIARHPEKKQHNAGPASNYEDERKRRVVEAEKGVRLFLATRVVGVERVGG
                     RIVSVTGRDIVTNRERRFRGRLFADCTGDAGVGALAGADFRVGREARGETGEGLAPER
                     ADTLMMGVSVQWYSETTDGPAPFPDCPWALPFTDQTVQALKRGDWDWETGMTRDSVTE
                     IEAIRDHALRATFGNWAYLKNHSAKRQAFANQRLAWVAYIGGKRESRRLLGDVILSQQ
                     DITGQRPYPDACVTTTWPLDLHYPHPENEKHFPGMAFRAVSKSEGIKPYAIPYRCLYS
                     RNIENLFMAGRNISVTHVALGSVRVMRTTGMMGEVVGMAASLCIRHDTTPRGVFETRL
                     EELRERMRRGVARGEGQASACP"
     gene            complement(4826..5515)
                     /locus_tag="PLZ74_07270"
     CDS             complement(4826..5515)
                     /locus_tag="PLZ74_07270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR98158.1"
                     /translation="MERAAFLFDIDGTLLYARGIGRAAFGTAFETAYGVPYPDLAGLT
                     FVGATDSNVVRTMAATCGVASTPAREEHFFLLLTQAIDAGLAQTKPLVYPGVPALLDR
                     LCKEGYPLGLITGNIRATAWAKLRHAGLDAAFCFGAYGDDDPDRNVIARLALQRVPHG
                     VRARVLIGDTPLDIQAAHANGLIAVAVATGWVTADALAAAGADLVLDDFSDTETVLAQ
                     LKALLASCNPG"
     gene            5610..6557
                     /locus_tag="PLZ74_07275"
     CDS             5610..6557
                     /locus_tag="PLZ74_07275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOR98159.1"
                     /translation="MGNMRRRNFLKVTFGALTVGPVVARAAAFGAYDRVRLGRSGIET
                     SRLCFGTGYKAFNRVSEQTRLGHDAFVALLRAGYERGIRCFDAADLYGSHRPLAEALK
                     PFPRDRYTLISKIWWRKGGVPEEDKTAVPEQIRRFLKELKTDYVDIVQLHCLTDGRWQ
                     TELADRMEALEACKKAGMIRAHGVSVHGFKALQACATSPWVDVAHVRLNPYGVKMEGT
                     VEANLPVIRAMRGKGQGIIAMKLLGEGAFSHDGEKINASLAFALRSGLADVLNIGFMS
                     TAQIDDIAARIAAVTPNAGGTRNPHPDPLQGAGEQTTSP"
     gene            complement(6735..9377)
                     /locus_tag="PLZ74_07280"
     CDS             complement(6735..9377)
                     /locus_tag="PLZ74_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOR98160.1"
                     /translation="MKEQSWGRILTALFIAFATTASAVPIGVTVNSVHLNDDASINDP
                     NWTYNSQTGVLTFHGSGNFMVSGTNTAGKARIVIPNGVTNTVRVWNLNLQVPGDDKCI
                     FALGTNACVSLILKGTNTLASGKDRAGIEVPKGASLSITSAAADEADMLTATGGQYGA
                     GIGGGNGIDGGTVIINSSVVTATGGGNGAGIGGGNGGKGGTVTINDGVVTATGGSAGI
                     GGGNGGKGGTVTISGGRVTATGGSNSAGIGGGNGGDGGTLTINGGRVAATGGDGSAGV
                     GGGNGGKGGTVTISGGSLFAQGKNGGADIGQGSNGTVSGANTFTGGAVHLANNSISVA
                     PTDGSQRVWCMTVTNLTPHALIVVTGLDPYGMNDLVADNAGKLRLWLPNGNYTFTAGG
                     AHYAATIDSADTTATLSALTPPDSDAYITFSSANTFEVKPKQGKSWNGILYYSTDTTF
                     WSVFTTDGATSATDGAGEHKLYLCGSGNSYITGNPDGPPWSITATAPVTCSGNLETLL
                     DHAKVNAGEHPAMAPYCFSYLFKGCASLVSAPSLPATNLTNHCYYNLFNSCTGLTNAP
                     ALPATTLPERCYNGMFNGCTNLTQAPALPATTLGKSSYGYMFYKCTGLTSAPALPATT
                     LANECYKNMFMDCTALTSAPALPATTLASACYDHMFSKCTGLTNAPALPATTLAPSCY
                     INMFSDCIGLMRVPELPAMNLAESCYNQMFRGCTNLTRPPELPATTLAVKCYYYMFYK
                     CTGIELNTAGPGMPWSIPANATAAPDWNRRMFYYTGGTLTGGPAIGTTYYLASGLPDA
                     PAFPTDGTGFVIGDGTATLAIINAEAGLWYTVYATDDLNATWQPVPGQSIRATGSHVA
                     FTFDIDTATVPRRFFKVAASLTTP"
BASE COUNT         1623 a         2769 c         3268 g         1733 t
ORIGIN      
        1 ctgaaaccct gaaaccctga aaccctgaaa ccgtgaaacc ccgaaaccca gaaacccacg
       61 aaagtgtccc gtcaggataa tccggggttt acctgtgcgc aacggttttg ttatgctttt
      121 tgcagatgag gtggtgtggc gcgggatccg gaaaaggctt tccgatgacg aaaaacaaga
      181 agaggagggg aaggaatgag gacgatgctg ttcgcctgtg tggcgggtgc ggtgctgaca
      241 acgcgggcgc aggtggaggc ggtgtggtta acgcaccgtt cggccacgcc ggaaaagacg
      301 gtggtgagct ggaagagcgt gacgccgggg ccgtcacgcg tggcctatgg tgcggacgcg
      361 gcctgcgggc agacggcgtc cgtcgcgggt gagcggagcg tgcaccatgt ggagatcgac
      421 acgccgcagc gcggggccac cgtgttctac cgggtggaga ccggagacca gaaatcggag
      481 gtccactcct ttcagacctg tccggaggac ggcgtgcgcg tggcgatcgt gggtgactgg
      541 ggatatgcga accgtccgga tctgaccgcg ctcaaggcgg acaagccgaa catcctcatg
      601 accgtgggcg acaatgtgtc cgatgtcatc aaccgcaagc aacccggcga catcacgaac
      661 atccggccct acatcggcct gctcaggtcg gaggcggcgt ttttcgcgtc cacactcttt
      721 atgccgattc tcggcaatca cgacaaacag gtcgggccgc gcgtcaacaa gcggccgggc
      781 tccagcaagg agacgtacga catcgacgcc acggcgtacc tgagcgtgtt cgcgctgccc
      841 gagccggggt ggcgctgggc tttcacgatc ccgcaggcgg acgtgacctt cctggcgctg
      901 gacatgcacc acctgtctga ttttggaacg accttccaga ccagtcacga ccaccggcag
      961 ggttccgagc agttcgcgtg gtacgatgaa cagacgcgta acctcaagac cggattcgcg
     1021 gtgacactgc tgaatgccga cagccggtgc aggaatcgcg agaaaggcgc gtggcaccag
     1081 atgttcagcc gcggcacgat ctttgtgacg ggatacgggt actatgccga gcgtgccgtt
     1141 gaacgcaacg ggttcccgta ctacaacacc tgcgtggccc ggccgggcga tgtctaccgc
     1201 gatccgctgg cggtcgtgtg tgagcgggtt tcaagctaca tgctgctgac ctttgataag
     1261 gccaaggggc agatgacggt ggacctcaag cgtctggacg ggagcctgat cgaccggcag
     1321 gtttaccacg cgaaaccgcg gtaagccgcc gtgcggccgc gccgcggata tccgcttggc
     1381 acaaccatca agatgttttg atgaggacgg aaaaaagggg ggggagcaca tgacatggac
     1441 ccggcgtgat ttcttgagcg cttcggccgg ggcggctctg gcttggcgat ccgttgcggc
     1501 gcaacagccg gttacggcgg cgcgggaggg atggctggag gccttccgcg cagcggggtt
     1561 cgatcccgct gcagcgggaa acagcgtgtt cgtggtgtgc ggggatgtgc attttccgca
     1621 gtacggccaa cacctccctt cgcagatcga ggcatggaac gccatgcggc ctgcgccgca
     1681 attcgtggct ctgctgggcg acaacgtgtg taacgtttcg agtagtttcg ggcacacgcc
     1741 tgatgcgaag gggctggagc gcgcacggaa agagttggag gggctgcgcg cggagaccgc
     1801 caagctccgc aaggagattc cgctcaagct ggtgatcggc aaccacgaca ccctgccgcg
     1861 ggagacggat gccgcattct tccggaaggt ctttcccgca tgccggccct acgaggcgtt
     1921 cgaggtttcc ggtatctgtt tccagatttg gaacggcgcg catgatggcg cgatcgatcc
     1981 ggcgcagcgt gtctggatcg ggaagcagtg cgctggcctg ccccgcgaca aaagcgtggt
     2041 gattctggtg catcagccct cgctcgggat gacggaacgc gagcgcggca tccccgccat
     2101 ggtgcgggcc cacttttcgg aacacaccgg gccgctctgg ttgctggcgg gccatgtgca
     2161 cagcaacagc atacgcgagt tcgcgttacc gcgtacccgg atcactcagg ccacgcatgt
     2221 caagagtgtg gacggctact ggatctacgg catgcgcgac ggccgtatca cggcgcgggt
     2281 gtactgtccg gtggcagggg cgtgcaaggc tcaggccatg cccgatccgg cgcggcccgc
     2341 acggccgatc ccgctgccgt tcgaggggcg cgacgatgtg gtttggaggc tgctgatcgg
     2401 tgaggacgcg acggcgatca agacggcctt tgtgcgcggc aagggcggcg attgcggcac
     2461 ctggtggttt tatgtgaacg agctggtcta taaactgccg ctggcgtccc ggggccgcgg
     2521 ggccacgcgc ttcgccgttc tggcggcgct gagcaaacat cgtaagaccg gggagctgcc
     2581 caaggtctac gcttctggca gcgatggcga ttggatcgag acaccgctgc tggaatcgcg
     2641 cggggccgag catctctttg ccatacccga ggggttgcgt aagagccgcg agttgtatgt
     2701 gcggattcag agctacgggt atggggcgga tacctgcgtt ggcggattcg cgctgtgccg
     2761 gtgagtcggt cacgcggctg cgggaaggat ggcatcgatt acggaaaggg cttatgaata
     2821 caataaaaag aaaattcctg tgccgcaggg gtatgatgtt gctggtggtt ttggggactg
     2881 gtgcggcggt tcaagccgcg accgtgctgg tcgaggcgga ggggttcgcg aacgcaggcg
     2941 gctgggtcat cgaccagcag tccatggacc agatgggttc gccgtatcgg atggcgcacg
     3001 ggttgggggt gccggtggcg gacgcggaga cctcggtgca ggtgccggaa gcggggcgtt
     3061 accgggtgtg ggttcgcaca cgtgactggg ttgccacatg gggcgcgccc ggcgcacccg
     3121 gcgcgttccg ggtattgatc aacggccgtg cgctcgatcc ggtcttcggc acacagcgcg
     3181 ccgagtggca ctggcagccg ggcggggaga tcgagttgcc ggtcggcccg gtgacgatcg
     3241 cgttgcgcga tctgaccgga ttcaacgggc ggtgcgacgc gattctgctg accacggatg
     3301 tcggcctgaa gccgcctgat ggcggcgggg agttggccgc gctgcgacgg caatggctgg
     3361 gcctgccgga cgcgccggaa gacgcggggg agtttgatct ggtggtcgtg ggcggaggca
     3421 tggccggatg ctgcgcggct gtgagcgcgg cccgtctggg ttgccgcgtc gccctgattc
     3481 aggaccgtcc cgtattgggc ggaaacaaca gttccgaggt gcgggtcggc ctttccggtc
     3541 agatccgtca gcagccctat ccgcggctcg gcgaactggt cgacgagatc ggtccggtcg
     3601 gtcactggaa cctgtacgac gccaaacgtg cgccggaact gccacgcagc cgcgaggtgc
     3661 tggagatcat cgcgcgtcac cctgaaaaga aacagcacaa cgccggtccc gcgagcaatt
     3721 acgaggacga gcgtaaacgc cgcgtggttg aagccgagaa aggggtgcgt ctgtttctcg
     3781 cgacgcgcgt ggtcggcgtg gagcgggtgg gcggacgcat cgtgtctgtg accggccggg
     3841 acatcgtgac caaccgcgag cggcgtttcc gcggcaggct gttcgccgac tgcacgggcg
     3901 acgcgggcgt gggggctctg gcgggtgcgg atttccgtgt cggccgcgag gcccggggcg
     3961 agaccggtga gggtttggcg ccggagcggg ccgatacact gatgatgggt gtgtcggtcc
     4021 agtggtattc tgaaaccacg gacgggccgg cccctttccc ggattgccct tgggccctgc
     4081 cgttcaccga tcagaccgtg caggccctga agcgcggcga ttgggattgg gagaccggca
     4141 tgacgcgcga ctccgtgaca gagatcgagg caatccgcga ccatgcctta cgtgccacct
     4201 tcgggaactg ggcctatctg aaaaaccaca gcgccaaacg gcaggcgttc gccaaccagc
     4261 ggctggcttg ggtggcctat atcggcggca agcgggaaag tcgacgcctg ctcggcgacg
     4321 tgatcctctc ccagcaggac attaccgggc agcgcccgta tcccgatgcg tgcgtcacca
     4381 caacctggcc gttggacctg cactatccgc accccgaaaa cgaaaaacac tttccgggca
     4441 tggcctttcg tgccgtttcc aaatccgaag gaatcaaacc ctacgcgatt ccgtaccgct
     4501 gcctttacag ccgtaacatt gagaacctgt ttatggcggg ccgcaacatc agcgtgaccc
     4561 atgtggccct gggttcggtg cgcgtgatgc gcacgaccgg tatgatgggc gaggtggtcg
     4621 gcatggcggc ttcgctttgc atacgccatg acaccacgcc gcgcggtgtg ttcgagacgc
     4681 ggctggaaga gttgcgggag cggatgcggc gtggtgtcgc ccggggggag gggcaagcgt
     4741 ctgcttgccc gtgaggacgg ttctccgaac cgtcagcgga cgcctcggga atgcgtccct
     4801 gccttcaaaa aatcgtccag aaaaattagc cgggattgca ggaagcgagc agtgctttca
     4861 gctgggccag cacggtttcg gtatcggaaa aatcatccag cacaagatcg gcgccggccg
     4921 cggcgagcgc gtcggctgtg acccatccgg tcgccaccgc caccgcgatg aggccattgg
     4981 cgtgggcggc ctgaatatcg agcggggtgt cgccgatcag tacacgggcg cggaccccat
     5041 gcggcacgcg ttgcagggcg agacgcgcga tcacgttacg gtccggatca tcgtccccgt
     5101 acgcgccgaa gcagaatgcc gcgtccagcc ctgcgtgtcg taacttggcc caggcggtcg
     5161 cacggatgtt gccggtgatc aatccgagcg ggtatccttc tttgcaaagc cgatccagca
     5221 gggcgggcac gccaggatag accagcggct tggtttgcgc gagcccggca tcgatggcct
     5281 gcgtcagcag cagaaagaag tgctcctcgc gcgcgggcgt gctggccaca ccgcacgtcg
     5341 cggccatggt gcgcaccacg ttggagtcgg tcgcgcccac gaaggtgagc cccgcgagat
     5401 ccgggtaagg tacgccgtac gcggtctcga acgcggtgcc gaacgcggcc cgtccgattc
     5461 cccgcgcata gagcagggtt ccgtcaatat cgaataaaaa agcggcacgt tccatgatgg
     5521 gattgaaaac cgggcccgtc cgggctacaa tgtgcctgaa ctatatcaca cgcgccgggt
     5581 acaggcgacg ggaaacagag gggtgaggca tggggaacat gcggcgcagg aattttctca
     5641 aggtaacgtt cggcgcgctg accgtcggcc cggttgtggc gcgcgcggcg gcgttcggcg
     5701 cgtatgaccg ggttcggctc ggacggagcg gcatcgagac ttcgcggctc tgtttcggca
     5761 ccgggtacaa ggcgttcaac cgcgtctccg agcagacgcg gctggggcat gacgcctttg
     5821 tggccctgtt gcgcgcgggg tacgagcggg ggattcgctg tttcgatgcc gccgacctgt
     5881 acgggtcgca ccgaccgctg gccgaggcgc tcaagccctt tccgcgcgac cgctacacgc
     5941 tgatctccaa aatctggtgg cgcaagggcg gggtgccgga ggaggataag accgcggtac
     6001 cggagcagat ccggcgtttc ctgaaagagc tgaagaccga ttacgtggat atcgtgcagt
     6061 tgcactgcct gaccgacggg aggtggcaga cggaactggc cgaccggatg gaggcgcttg
     6121 aagcgtgcaa gaaggccgga atgatccgcg cgcacggcgt gtccgtgcac gggttcaaag
     6181 cattacaggc atgcgcgacc tctccctggg tcgatgtggc ccacgtccgg ctcaatccgt
     6241 acggcgtcaa gatggagggc acggtggagg cgaatcttcc ggtcatccgc gcgatgcgcg
     6301 ggaagggtca ggggatcatc gccatgaagt tgctgggcga gggtgccttc tcgcatgacg
     6361 gggagaagat caacgcttcg ctcgcgttcg cgctccgcag cggactggcc gatgtgttga
     6421 acatcgggtt catgagcacc gcacagatcg atgacattgc ggcgcggatc gccgccgtga
     6481 cccccaatgc gggtggaacc cgcaaccctc accccgaccc tctccaaggg gcgggggagc
     6541 aaacaacttc cccgtaaacc gccgccgggc aagcagacgc ttgcccctcc cgttgacccg
     6601 cgactggcga cttgcgtcaa gcaaacaaaa cgtcgcaggt cgaatgtcgc gggtcgcggg
     6661 tcaacgtaca cgaaaacgtg tgcgatcccg atcccggtgc ttcgaaaact tatcacttcc
     6721 acgcttcaca gcgcctaggg cgttgtgagg ctggctgcca ccttgaagaa gcggcggggg
     6781 actgttgccg tgtcaatatc gaatgtgaag gcaacgtggc tgccggtcgc gcggatgctc
     6841 tggcccggta ccggctgcca ggttgcgttg aggtcgtccg tcgcatagac cgtgtaccag
     6901 aggccggctt cggcgttgat gatcgcgagg gtggccgtgc cgtcgccgat gacgaatccc
     6961 gtgccgtccg tggggaaggc tggcgcgtcg ggaaggccgg acgcgaggta gtacgtggtg
     7021 ccgatcgccg ggccgccggt taaggtgccg cccgtgtagt aaaacatccg tctgttccag
     7081 tccggtgccg ccgtcgcgtt ggccgggatg ctccacggca ttccggggcc tgcggtgttc
     7141 agctcgatgc ccgtgcattt gtagaacatg tagtaatagc acttcacggc cagagtcgtc
     7201 gccggcaatt ccggcggacg cgtcaggttc gtgcaacccc tgaacatttg gttgtagcag
     7261 ctctcggcca gattcattgc cggcaattcc ggcacgcgca tgagcccaat gcagtcgctg
     7321 aacatgttta tgtagcaaga gggtgcaagc gtcgtggcgg gcagcgccgg cgcgttggtc
     7381 agccccgtgc atttggagaa catgtggtcg tagcaggctg acgccagtgt cgtggcgggc
     7441 agtgccggtg cgctcgtcag tgccgtacag tccatgaaca tgttcttgta gcattcgtta
     7501 gccagcgtcg tggccggcag tgccggcgcg ctcgtcagtc ccgtgcattt gtagaacatg
     7561 tagccgtagc tggatttgcc cagcgtcgtg gcgggcagcg ctggcgcctg cgtcaggttc
     7621 gtgcatccgt tgaacatgcc gttatagcat ctttccggca atgtcgtggc cggcagtgcc
     7681 ggcgcgttcg tcagccccgt gcaactgttg aacaggttat agtaacaatg attggtcaga
     7741 tttgtcgccg ggagcgatgg tgcgctgacg agcgacgcac agcctttgaa gaggtacgag
     7801 aagcagtagg gcgccattgc cggatgctcg cctgcgttca ccttcgcatg gtcgagcagg
     7861 gtttcaaggt tgccggagca cgtgaccggc gccgtggcgg taatgctcca gggggggcca
     7921 tctgggttgc cggtaatgta actgttgccc gatccgcaga gatacagctt atgctccccg
     7981 gcaccgtcgg tagccgaggt cgcgccgtcc gtggtgaaga ccgaccagaa cgtcgtgtcg
     8041 gtggagtaat acagtatccc gttccatgat tttccttgct tcggcttgac ctcgaaggtg
     8101 ttcgcgcttg agaaagtgat gtacgcgtcc gagtctgggg gcgtcagcgc tgacaaggtc
     8161 gctgtcgtgt ccgcactgtc gatggtcgcg gcgtagtgtg cgccgcccgc cgtgaaggtg
     8221 tagttgccgt tgggcagcca gaggcggagt ttgccggcat tgtctgcaac gaggtcgttc
     8281 atgccgtagg ggtcgaggcc ggtgacgacg atcaacgcat ggggcgtgag gttggtcacc
     8341 gtcatgcacc agacgcgttg tgagccgtcc gtgggcgcga cggagatgct gttgttcgcc
     8401 aggtggaccg cgccgccggt gaaggtgttc gcgccggaga ccgtcccgtt gctgccctgt
     8461 ccaatgtccg cgccgccgtt cttgccttgc gcgaagagcg agccgccgga gatggtcacc
     8521 gtgccgcctt tgccgccatt gccgccgccc acgcctgcac tgccgtcgcc gccggtggcc
     8581 gcgaccctgc cgccgttgat ggtcagcgtg ccgccgtcgc cgccattgcc gccgccgatg
     8641 cccgcgctgt tgctgccgcc ggtggccgtg accctgccac cggagatggt cactgtgccg
     8701 cctttgccgc cattgccgcc gccgataccc gcactgccgc ctgtggctgt gaccaccccg
     8761 tcgttgatgg tcaccgtgcc gcctttgccg ccattgccgc cgccgatgcc cgcgccgttg
     8821 ccgccgcctg tggccgtgac cacgctgctg ttgatgatca ctgtgccacc gtcaatgcca
     8881 ttaccgccgc cgatgcccgc gccgtactgg ccgcccgtgg ctgtcagcat gtccgcttcg
     8941 tcggctgccg cactggtgat cgagagtgat gcgcccttcg ggacttcgat gcctgcgcga
     9001 tccttccccg aggccagcgt gttcgtgccc ttgaggatga gcgagacgca ggcgttcgtc
     9061 cccagcgcga aaatacactt gtcgtcgccg ggcacctgaa ggttcaggtt ccagaccctg
     9121 accgtgttcg tgacgccgtt cgggatgacg atgcgcgcct tgcctgccgt gttggtgccg
     9181 gagaccatga agttgcccga cccgtgaaac gtgagaacgc cggtctgcga gttgtacgtc
     9241 cagttcggat cgttgattga ggcgtcgtcg ttgaggtgga cgctgttcac cgtcaccccg
     9301 atcgggacgg cgctggcggt ggtcgcgaag gcgataaaga gcgcggtgag gattctgccc
     9361 caactctgtt ctttcatggc ggtgttcctt atc
//
LOCUS       DAOSRB010000114         9246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_7158_length_9246_cov_6.190948, whole genome shotgun sequence.
ACCESSION   DAOSRB010000114 DAOSRB010000000
VERSION     DAOSRB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7158_length_9246_cov_6.190948"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2622
                     /locus_tag="PLZ74_07285"
     CDS             <1..2622
                     /locus_tag="PLZ74_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98161.1"
                     /translation="ADLTCVFTQKGGTFTALDSIGITLAYAQGRKTKLVLDGGVMSVP
                     QIIGGTGAAVNGGTGLALLAANGGTLVARRDNATFVHALDGVELGEQGLVVDTDYDVT
                     ITAAISSKSNEKGVLVKRGRGTLTLAGTVADTVKVEVQGGKVVFGQSVTLDALTLGTE
                     NSSGAIELADGVTLAITGAVTINHNASMQEFVFEEDLNGVTHVTVDDSPAETLEIRLD
                     EAVTSNATAAVEFRAKDTLNVSVVSGGTLNLGGKLTRGALVTKGGGHAVLQNTGNEFY
                     GGVTVESGWLSFTDARALGLTFSDAATLTLKGGTFEYMGTTASAIPSKLSMASAVTTN
                     PIVVKTSGDMTFGPFSAAGGALIKAGPGKVTLAAKNGNTVVTSGAGIGTGPNGCPRYG
                     FALDPNGGASAGEYAALNVVEGELVLQGAGNVKSAGGVVAIGLNAAEGVAQPILTVDE
                     AYLDNNQHFFVGGNIGASAFATNAMLQITNGGTVKCDTLRVGTMTSGKPSYPEVRVNG
                     GTLDCGFRLNCADEQTWTRARYLFENGATLKIGSYNYSSWQLYWEGEAAFTFDASSYA
                     IAGNNHYADVHVRQAGRGSMLFRNNSTFYGEKITKWSGDNYKLTFAFDNGEWIPGTGD
                     YDFTFTGGLGIIVDCRAGGLTLNVPATKTWTVGQKISGTGGIVKKGTGTLAFTPQATI
                     VEGARTLVADDPVSWAFADTLKVESGTLAVANDAATPFASAELAAGTVFNPGSALVFG
                     EVSGSGTISGGGLVATTLRAAELATLTLSGVTLTSPTFKPAVDASGTLTGHFVLTNCD
                     YDGQVIVDFGRSELDPLDAGTTYEIGTVTGTLPDLARWRVVNTGIAQGAGDFSPVGED
                     GKIRVTLSRRGFLMTVR"
     gene            2638..5346
                     /locus_tag="PLZ74_07290"
     CDS             2638..5346
                     /locus_tag="PLZ74_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98162.1"
                     /translation="MRTRFWGTVAAFCCVGSATFAAVAPGVYPADEVPVARGSIELWV
                     TPNQDGCDARDHVYFADADAWAREGTPTIWYWAEYVRFDVDSGKRLVYGRLADGLLEP
                     DVPVHIVASWDAAKGQTAVYANGELVQRREVEPWVPVALERYVVGEDCSRRKKAGAVI
                     DALHFFAVPLTPGEIKTRFDAKKNLVDSARSSAARKKHMPPPLPAKATVAPEWQVVAT
                     IDPYSEKPCAAIGETKVGEIPGCRFRETGIKSHDRFAYVRELPATNLLLRVRITYPDD
                     TYRSLMVSQTQPDFGEIRTAGSEQQVLGSGILTGSEFPVTGKPLVYEYVFRHPSTWLG
                     LIFEGYQPDKPGAVGPVTIAYAAPEKDRYGPAAPLPSLAAHKARKAGLYWEDPVLSMC
                     FGGVGGVDPATYDRDLVSAMDYFSWVGQDLWFYPTVWYNGPVYRSRKEKGTWPSGMRH
                     HPADFPHLFAARCAERDITFVPSFGMSRLPTLNLKFGTREQVIAGDQTVLNVVYADGE
                     IPVTPFMYKPPIFNALRRETQDAVMGLVDEHCDMLADLPSFGGISFYLNHWNLTQLGL
                     WLDGSYDDWTMETFAKGIGEILPGTPGDAQRFKIRADWIRNDPARRNRFLRWRAEIVS
                     GFYEKVAARIRSRNSDAKLYLRLDVPSISQMCTSGGRFCKPFSDESFLEMGLDVPRLA
                     KHEAVCLDRLFSWGIVRCERKYWPDEKLVPPLFDLEPAFQKEVLGAVDAVTIHQYYFE
                     THGEFSRGRKLTMPAPWKNEAPGRCTAPLYPGRWPLVYYARALALFDMKEVSIGGFTL
                     GTHGIEDLVRTWTASFRSLPLVDFGEEVRFDDVILRTAVAEGARWWYMLNTASQERVI
                     PLSVKGVCRETVYGDRVKSGSRTLKSFELVAVRGEMDSQLTIKETP"
     gene            5343..8063
                     /locus_tag="PLZ74_07295"
     CDS             5343..8063
                     /locus_tag="PLZ74_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98163.1"
                     /translation="MKVLFMCLGLLACGCRFADKAFAEEQADFWVAPDGKDTDPGTKE
                     RPFATLARARDAVRALRQAEPDRDILVLFRSGTYPIEGPIGFSPEDSGRAGGRITYAA
                     APNATPVISGGRRITGFEPCGDGIWKTTVPNARDGKWRIEQLYVNGSRAVRARTPNGH
                     YAYIDSIVETGIDPATGKAGPIRDRAFLGRPKDIAPLLKLPQERLRDVVVSAYHSWEI
                     SRHRIVSIDPGRNLVLLTGRYPPKFGHYTSRERYYLDNFREALDAPGEWFLDRDGTLF
                     YKPRSGEDMAQAEVVAPVTETFLRFAGSAGGAQVENILFRGLTFEYAAYLMPHEGAWS
                     PQAACNIDAVVMADYARNIAFTDCAIRRTGTYGFWFRDGCRDCVVERCLLEDLGAGGV
                     RLGPTAHRPHDAGQLTSHCRIDNNIIRDAGRVWPDAVGVFIGHSGDNQVTHNDIAGMP
                     YSGISVGWRWGYGEVPSKRNTISYNRIHHLGWDVLSDMGGIYTLGEAPGTVMSGNVIH
                     DFSGDGECSMHGIYNDNSTAYMLMESNLVYNVRDGGYKLGSGKANIVRNNIFVSRLRD
                     KPRHSGPIMFCQYYTNETHVAATIEKNIVCGCEGQIFASSEFANRLELRNNVYWEPSG
                     TPIDFIGRSFEAWQKLGRDAGSVIADPKFADADNNDFHLQADSPALALGFVPFDSSRA
                     GVYGDPAWIAKAREFAVPPPWQGLPPPPPLSFSDGFEHTDVGSLPRRATSMVENKGDG
                     IEVTEETAATGKRSLKITDAPGLKHGFNPHFWYTPNHHEGVTTFSFDVHMEQGAELSL
                     EWRQYPGKPYYYIGPKLLFRDGKLTFAGQPPLPLPLGVWTHIEISAGLGSKTDGTWQL
                     TVTPQGAEPKRFSGLKFGNPETRSVTWIGFISVANRKGVYYVDNVKLENN"
     gene            complement(8633..>9246)
                     /locus_tag="PLZ74_07300"
     CDS             complement(8633..>9246)
                     /locus_tag="PLZ74_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98164.1"
                     /translation="MSRVDFGWWYFFSPSGGARPTVGTQMDMWEYGASVAAAWDCAIS
                     ILMPTSALKRHPRTDDILEMMRRWGDCRRRGLFKDEWRPRLKNYAQEHHLIVDAKGNY
                     DLVDYSQIVVGNGQNPVRAFVFAKDNATWVVYWHGSGEGRLRLPIAAGAATLHDDFAG
                     KAVAFEQDGASIIVPATKRLYLKTTLTRDAVERAFAAATLLSK"
BASE COUNT         1838 a         2548 c         2910 g         1950 t
ORIGIN      
        1 gcggatctga catgcgtctt cacccaaaag gggggaacgt tcacagcgct tgattcgatc
       61 ggcatcacgc ttgcgtacgc gcaggggcgg aagactaaac tcgttctgga cggaggcgtg
      121 atgagcgtgc cgcaaattat tggcggcacg ggggcggcgg tcaatggcgg tacaggcctt
      181 gcgctgctcg ccgccaatgg cggaacgttg gtggcgcgac gtgataacgc gacgttcgtg
      241 catgcgctgg atggagttga actcggtgag caggggctgg ttgtcgacac agattacgat
      301 gtgacgatca cggcggcaat ctcttcgaag agcaacgaga agggggtctt agttaagagg
      361 ggacgcggca cactgacgct cgccggaact gtggcagaca cggttaaggt cgaggtgcaa
      421 gggggcaagg ttgtgttcgg ccagtcggtt acgcttgatg ccctgacgct cggcaccgaa
      481 aactcctccg gcgcgatcga actggcagac ggtgtgacgc tcgccataac cggcgccgtg
      541 acgatcaatc acaacgcctc aatgcaggag ttcgtttttg aagaagactt gaacggtgtg
      601 acgcacgtga cggttgatga ttcgccggcc gagacgcttg agatccgtct tgatgaagcc
      661 gtgacatcca acgcgacggc ggcagtcgag ttccgtgcga aggatacgct gaatgtgtcg
      721 gtggtgtccg gcggcacgtt gaatctgggc gggaaactca cgcgcggcgc acttgtcacg
      781 aagggtgggg gacacgcagt ccttcagaac acgggtaacg agttctacgg cggcgtgacg
      841 gtcgagtccg gctggctctc tttcaccgat gcgcgtgcgt taggcctcac attttcagat
      901 gccgccacgt tgacgcttaa gggtggcaca tttgagtaca tggggacaac agcatcagcg
      961 atcccctcca agttgtcaat ggcgtctgcg gtcacgacga atccgattgt ggtgaagacc
     1021 tcgggcgaca tgactttcgg cccgttcagt gccgcaggcg gtgcattgat caaggccggc
     1081 cccggcaagg tcacgcttgc ggcgaagaac ggcaacacgg ttgtgacgtc tggtgccggg
     1141 atagggacgg gtcctaatgg atgcccgcgg tacggtttcg cattagatcc gaacggcggt
     1201 gcgtcggcag gcgagtatgc cgccctgaat gtggttgaag gtgaactcgt cctccaaggt
     1261 gctggcaatg taaagtctgc cggtggtgtg gtggcgatcg gcctcaacgc cgcagaggga
     1321 gttgcgcagc ctatattgac cgtggatgaa gcatatctgg ataacaacca gcactttttc
     1381 gtcggcggaa atatcggcgc gagcgccttc gcgacaaacg ccatgctcca aatcaccaac
     1441 gggggcacgg tgaagtgcga cacgctgcgt gtcggcacaa tgacatccgg caagccctcc
     1501 tatccggagg tacgagtgaa cggcggcaca cttgattgcg gatttcgcct gaattgtgcg
     1561 gacgaacaga cttggacgcg cgcacgctac ctttttgaga acggcgcaac gctcaaaatc
     1621 ggctcataca actattcgtc ttggcagttg tattgggagg gagaggcggc attcacgttt
     1681 gatgccagct cgtacgccat tgcgggcaac aatcattacg ctgatgttca tgtgcgccag
     1741 gcggggcggg gatcgatgct gttccggaac aactcgacat tctacggtga aaagatcacg
     1801 aagtggagcg gtgacaatta caaactgaca tttgccttcg ataacggcga atggataccc
     1861 gggactggtg actacgactt cactttcacg ggcggtttgg gaatcatcgt tgactgccgg
     1921 gcaggcggat tgacgttgaa cgtgccagcg accaaaacgt ggacggtcgg acaaaagatt
     1981 tccggtacgg gagggatcgt caaaaaaggc actggcacac tcgcgtttac gccgcaggcc
     2041 acgatcgtcg aaggagcgcg cactctggtc gcagatgatc cggtctcgtg ggcgttcgca
     2101 gacacgctga aagtggagag cggtacgctt gcagtcgcga acgatgctgc gactccgttc
     2161 gccagcgccg aacttgccgc agggacggtc ttcaatcccg gttcagcgct tgtgttcgga
     2221 gaggtttccg gcagcggtac aatatccggc ggtggtctgg tggcgacaac cctccgcgct
     2281 gctgaacttg cgacattgac gctgtcgggg gtgacgttga cttcgcccac gttcaagcct
     2341 gcggtggacg catcaggtac gctgacggga catttcgtcc tgacgaattg tgactacgac
     2401 ggccaagtga tcgttgattt cggaagaagt gagctggatc cgttggatgc cgggacgacc
     2461 tacgaaatcg ggactgtcac cgggactctt cctgacctcg cgcgttggcg ggtcgtcaac
     2521 accggcattg cgcaaggcgc cggggacttt tcgccagtgg gagaggacgg aaaaattcgg
     2581 gtgaccctgt cgcggcgcgg gttcctgatg actgtgcgct gaggcggagg acgcttggtg
     2641 agaacccggt tttgggggac agtggcagcg ttttgttgtg tcggcagtgc gacatttgca
     2701 gcggttgcgc ctggcgtgta tccggcggac gaggtccctg ttgctcgcgg atcgatcgag
     2761 ttgtgggtta cgccgaatca ggacgggtgc gacgcgcggg atcatgttta ttttgcagat
     2821 gcggacgcat gggcgcgcga gggtaccccc acgatttggt attgggctga atatgtgcgc
     2881 ttcgacgtag actccggcaa gcggctggtt tatggccgac ttgccgatgg cttacttgaa
     2941 ccggatgtgc ccgtacatat tgtggcatct tgggatgcgg cgaaggggca aaccgcagtg
     3001 tacgcgaacg gtgaactcgt gcaacggcgt gaagtcgagc cgtgggttcc cgtcgcgttg
     3061 gagcgatacg ttgtcggcga ggattgctcc cgcaggaaaa aggcgggtgc ggtgattgat
     3121 gcgttgcatt ttttcgctgt gccgttgaca cccggtgaaa tcaaaactcg atttgatgct
     3181 aagaaaaacc ttgttgattc ggcgcgctcg tctgccgccc gaaaaaaaca catgcccccg
     3241 ccattgcccg ccaaggcaac tgtcgcgccg gagtggcagg ttgttgcgac aatcgatccc
     3301 tattcggaaa aaccctgtgc tgcaatcggc gagacaaagg ttggcgagat tcccggatgc
     3361 aggtttcgcg agaccggaat aaaatcgcat gaccggttcg cgtatgtacg tgagctaccc
     3421 gcgacgaatt tacttttgcg cgtgaggatc acttatccag acgacaccta ccgttcgctg
     3481 atggtgtcgc agacgcaacc tgatttcggc gagatccgta cagcgggttc cgagcagcag
     3541 gttttgggtt cgggcatcct gacgggtagc gagttccccg tcacaggcaa gccgcttgtc
     3601 tacgagtatg tgtttcgcca cccgtcaacg tggttggggt tgatctttga gggataccag
     3661 ccggataagc ccggtgcagt ggggccggtt acgattgcgt atgccgcgcc tgaaaaagac
     3721 cggtacggcc cggccgcgcc gctcccttcg cttgcggcgc ataaggcccg caaggctggc
     3781 ctgtattggg aggatcctgt tctttcaatg tgcttcggtg gcgtcggggg cgtcgatcct
     3841 gcgacgtatg accgtgatct tgtgagcgcc atggattatt tctcgtgggt aggccaagac
     3901 ctgtggttct acccgacggt gtggtacaac ggccccgttt atcgctctcg caaggaaaag
     3961 ggaacatggc cgagcgggat gcgccaccat ccggcggact tcccgcatct cttcgcggcg
     4021 cgttgcgcgg agcgagacat cacctttgtg cccagtttcg ggatgagccg tttgccgaca
     4081 ttgaacctga agttcggcac gcgagaacag gtgatcgcag gtgaccagac ggtattgaac
     4141 gttgtttatg cggatggcga gatccccgtg acgccattta tgtacaagcc cccgattttt
     4201 aacgcattgc gtcgcgaaac gcaagacgcg gtgatggggc ttgtcgacga acactgcgac
     4261 atgcttgccg atttgccgtc gttcggagga atttcattct atctgaatca ttggaatctc
     4321 acgcaactgg ggttgtggct ggacggctca tacgacgatt ggaccatgga gacgttcgca
     4381 aaaggaatcg gcgagatcct ccccggcacg ccgggtgatg cgcaacgctt caagatccgg
     4441 gctgattgga tcaggaatga cccggcacgc cggaaccgat tcttgcgttg gcgcgctgaa
     4501 attgtgagcg gcttttacga aaaggttgcc gcgcggattc gctcgcgaaa ttcagatgcg
     4561 aaactgtatc tccgtttgga tgtcccttcc atatcccaga tgtgcacatc cggcgggcgt
     4621 ttctgtaaac cgttttccga tgagtcgttt ctcgaaatgg gactggacgt cccgcgcctt
     4681 gcgaagcatg aggcggtttg tcttgatcgg cttttcagtt gggggatcgt gcggtgtgag
     4741 cgcaaatatt ggccggatga gaagttggtg ccgccgctct ttgatttaga gccggcgttt
     4801 cagaaagaag tgctgggggc tgttgatgcg gtgacgattc accaatatta ttttgagaca
     4861 cacggtgagt tttcccgcgg acgcaagctt accatgcccg cgccgtggaa gaatgaggca
     4921 ccgggacgct gtacggcacc gctctatccc ggacgttggc cgctcgtgta ttatgcccgt
     4981 gctttagcgc tcttcgacat gaaagaggtg tcgatcggtg gtttcacgtt gggtacacat
     5041 ggcatcgagg atttagtgcg gacatggacg gcgtcgttcc gcagtttacc gctcgttgat
     5101 tttggcgagg aggtgcgttt cgacgatgtg attttgcgga cagccgtggc agagggggca
     5161 cgctggtggt acatgctgaa cacagcaagc caggaaaggg tgatcccgtt gtcggtcaag
     5221 ggtgtgtgcc gtgaaacggt ttatggtgat cgtgtgaaat ccggctcccg cacgttgaaa
     5281 tcttttgagt tagtcgcggt tcgcggagag atggattcac agttgactat caaggagacg
     5341 ccatgaaggt tctgttcatg tgtttgggac tgttagcctg cgggtgccgt tttgccgaca
     5401 aggcgtttgc cgaggagcaa gccgatttct gggtcgcgcc ggacggcaag gacaccgatc
     5461 ccggcaccaa ggagcgtccg ttcgccacgt tggcgcgggc gcgtgatgcc gtgcgcgcgc
     5521 tgcgtcaggc ggagccggac cgcgacatcc tcgtcttgtt ccgcagtggt acgtatccca
     5581 ttgaggggcc gatcggtttc tcaccggagg attcgggacg tgccggcggt cgcatcactt
     5641 atgcggctgc gcccaatgct acgcccgtga tcagcggcgg acggcgcatc actgggtttg
     5701 agccttgcgg agacggcatc tggaagacga cggtgccgaa tgcccgcgac ggcaagtggc
     5761 gcattgagca gctttacgtc aacggcagtc gcgccgtgcg ggcccgtacg ccgaacggtc
     5821 actacgccta catcgacagt attgtggaga ccggtatcga ccccgcgaca gggaaggccg
     5881 ggccgatcag agaccgcgcg tttctggggc gtcccaaaga tatcgcgccg ctgttaaagt
     5941 tgccgcagga gcggttgcgc gatgtcgtgg tctccgccta ccactcctgg gagatttcac
     6001 ggcaccgcat cgtgagcata gatcccggac gcaacctagt cctcctgacg ggccgctatc
     6061 cgccgaaatt cggtcactac acatcgcggg agcgctacta tctggataac ttccgcgagg
     6121 cgctggatgc gccgggcgag tggttcctcg accgcgacgg cacgctcttc tacaaaccgc
     6181 gttccggcga ggacatggcc caagcggagg tcgtggcacc ggtgacagaa acctttcttc
     6241 gtttcgctgg cagcgcgggg ggtgcgcagg ttgaaaacat cctgtttcgc gggttgacct
     6301 tcgagtacgc cgcttatctc atgccgcatg aaggcgcgtg gtcgccgcag gccgcctgta
     6361 acatcgatgc cgtcgtgatg gcagactacg cgcgcaacat cgctttcacc gactgcgcga
     6421 tccggcggac cggcacctac gggttctggt tccgcgatgg atgccgcgac tgtgtcgtgg
     6481 agcgctgcct gctggaggat cttggtgctg gcggtgtgcg gttggggccg accgcgcata
     6541 ggccgcacga cgccgggcaa ctgaccagcc actgccgcat cgacaacaac atcatccgcg
     6601 atgccggccg cgtgtggccg gacgccgtcg gtgtttttat cgggcacagc ggcgacaatc
     6661 aagtcacgca caacgatatc gcgggaatgc cttactccgg tatttccgtc ggctggcgct
     6721 ggggttacgg cgaggttcca tcaaaacgca acaccatctc gtataacagg attcatcacc
     6781 ttggatggga tgtgctgtct gacatgggcg gcatctatac gctcggagaa gcgcccggga
     6841 cggtgatgag cggcaacgtc atccatgact tcagtggtga cggagaatgc agcatgcacg
     6901 gtatttacaa cgacaacagc accgcctaca tgctcatgga gagcaatctg gtctacaacg
     6961 tgcgcgacgg cggatacaag ctaggcagcg gcaaggccaa cattgtgcgc aacaacattt
     7021 tcgtctccag actccgcgac aaaccacggc atagcggtcc gatcatgttc tgccagtact
     7081 acacgaacga gacgcacgtc gcggctacga tcgagaagaa catcgtttgc gggtgcgagg
     7141 ggcagatctt tgcctcctct gagttcgcga atcggcttga actgcggaac aatgtatact
     7201 gggaaccatc gggcacgccc atcgatttta tcgggcgctc gtttgaagcg tggcaaaaac
     7261 tgggccgcga tgccggttca gtcatcgccg atccgaagtt cgccgatgcc gacaacaacg
     7321 atttccatct gcaagcggac tctcccgcgc tggctctcgg cttcgtgccg ttcgactctt
     7381 cgcgggccgg cgtctacggc gatcccgctt ggattgccaa ggcccgcgag ttcgccgtgc
     7441 cgccgccgtg gcaggggttg ccgccgcctc cgccgttgtc attctctgac ggctttgaac
     7501 acacagatgt cggtagcctg cccagacgcg ctacctccat ggtcgagaac aagggggacg
     7561 gcattgaggt aaccgaagag accgccgcaa ccggtaagcg gtcgctgaaa ataaccgatg
     7621 cacccggatt gaagcatggc tttaacccgc atttctggta cactcctaac catcacgagg
     7681 gcgtgacaac cttctccttc gacgtccaca tggagcaggg cgcggagctg agtcttgagt
     7741 ggcgtcaata tccgggcaag ccgtactact acatcgggcc gaagctgttg ttccgtgacg
     7801 ggaaactgac cttcgccggc caaccgccgt tgcccctgcc gctcggcgtc tggacgcata
     7861 tcgagatcag cgcgggcctc ggctcaaaaa ccgacggcac ctggcagctc acggtgaccc
     7921 cccagggcgc ggagcccaaa cgtttttcag gcctgaagtt cggtaatccc gagactcgca
     7981 gtgtcacatg gatcgggttc atcagcgtcg cgaatcgtaa gggcgtgtat tacgtcgata
     8041 acgtgaagtt agagaacaat taaccgacgt tcaccacgag tagagcaacc ccgtcttcga
     8101 tgtccagcat agcattgatg aggtgtacgg tcttgaagag gtgaaggtcg ctcaccgtga
     8161 tctccgcttc actctcttgt ttcgcaactc ggccgatagc cgagccacca agtcgggcaa
     8221 ttggggtgcc ctgaatccga aatgcgaggc ggtctcgcgc atccgctctc tgtgtgcctc
     8281 accgtcttgc tcgataaagc gaatcagcac gcccgagtcc agttcttcgc aacagtgctt
     8341 ttactctcac gactgcaagc cactacgtgg ccgcaataag agaggttgac tcgcgacttg
     8401 cgacttgcgg ccagccctgt ccccccaaaa acctctgcgc cttctgcgtt aaactccttg
     8461 cctataaatt gacaggatga caggattttc aggattgaac aacatcatgt taatcatgga
     8521 tttctccccg ccgaagcgaa tctgtcggaa atcatgtcct aaactcacat ccaaggtttt
     8581 ttcgcggcag agccgcgcgg cgggctaaat cctcaatcct aaatcctcaa tcctacttgc
     8641 taagcagcgt cgccgctgca aaggcccgct cgaccgcgtc gcgtgttagc gttgtcttga
     8701 ggtaaaggcg tttcgtcgcg ggcacaatga tcgacgcgcc gtcctgctcg aacgcgacag
     8761 ccttgccggc gaaatcgtcg tggagggtcg ccgcgcctgc cgcgatcggg aggcgcaacc
     8821 gtccctcgcc cgagccgtgc cagtagacga cccatgtggc gttgtccttt gcgaaaacga
     8881 aggcgcgcac cgggttttgg ccgttgccga caacgatctg ggagtagtcg acgagatcgt
     8941 aattgccttt ggcgtctacg atgaggtggt gctcctgcgc gtagttcttg agccgcggac
     9001 gccactcgtc tttgaaaagg ccgcgcctgc ggcagtcgcc ccagcgccgc atcatctcaa
     9061 ggatgtcatc cgtacgcggg tggcgcttca acgccgatgt cggcatgaga atcgatatcg
     9121 cgcagtccca cgctgccgcc acggaggcgc cgtattccca catgtccatt tgggtgccca
     9181 ccgtgggccg cgcgccgccg gaagggctga aaaaatacca ccagccgaag tccacgcgcg
     9241 acatgt
//
LOCUS       DAOSRB010000115         9007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_7362_length_9007_cov_5.805630, whole genome shotgun sequence.
ACCESSION   DAOSRB010000115 DAOSRB010000000
VERSION     DAOSRB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7362_length_9007_cov_5.805630"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLZ74_07305"
     CDS             <1..483
                     /locus_tag="PLZ74_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOR98165.1"
                     /translation="MVHACLYAAAENSGKLVATVRREARRIAEAKDLPLIITDGPPGI
                     GCPVIASLTGASKVLAVTEPTVSGEHDLERVLQLTRFFEVPVAVCVNKWDINPDVTER
                     IERKAENLGAACIGRVRYDKAVTTAQIQRKSVVEIGSAAGDDIRQIWERMEGSWRERA
                     "
     gene            599..952
                     /locus_tag="PLZ74_07310"
     CDS             599..952
                     /locus_tag="PLZ74_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOR98166.1"
                     /translation="MNTNKIKIAIPLAEGMLCMHFGHCETFALLDVDLTGRKIIRRED
                     AVPPPHEPGVLPRWLAEQKVEMVMAGGMGQRAQGLLAGQNIRVIVGLSPDTPENIIAQ
                     YLDGTLKSGENVCDH"
     gene            961..1791
                     /locus_tag="PLZ74_07315"
     CDS             961..1791
                     /locus_tag="PLZ74_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR98167.1"
                     /translation="MGQKVSITILADNLAAEGLASEHGFAAWIETDGAKILFDTGNGD
                     AMMQNAETLGIALPHADMIVLSHGHNDHTGNLATILQQAPQARLYLHPEALRQRYSIR
                     ESAKPIGMPASAVNAVSGLAEHRRMWVTATVQISEKVRLTGPVPRRTAYEDTGGPFFK
                     DAAGAHPDDIPDDMSLWIETRAGLVVCLGCCHSGVINTLNHITAVSGERRIRCVVGGT
                     HLVHASAERMKQTSAALKAYNIPRVVPCHCTGGDAVFFLKEEFGETVHQGYAGMRIDI
                     "
     gene            1977..2489
                     /gene="tsaA"
                     /locus_tag="PLZ74_07320"
     CDS             1977..2489
                     /gene="tsaA"
                     /locus_tag="PLZ74_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOR98168.1"
                     /translation="MSDNQIIFRPIGIIRTEHTNPEATPIQPIFAKDCEGCVMVAPEF
                     VAGLADIEDFSHVYLVYHLHKVSRVTLVTKPFMQDKEHGVFATRSPCRPNPIGLSIVR
                     LVSRDGGTLIVKGADMLDGTPLLDIKPYTRRFDNISTERNGWQDEVDDATAAKLGVRN
                     YCNRHLSSAE"
     gene            2593..3507
                     /locus_tag="PLZ74_07325"
     CDS             2593..3507
                     /locus_tag="PLZ74_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOR98169.1"
                     /translation="MNPEKTACDQSQCNACSDASCPSAGRQAQQPPMPDDARLKNRLS
                     RIRRKVVVMSGKGGVGKSTVAVNLAMSAMLSGQKVGLLDIDLHGPSVPVMLGLVGQRL
                     EGGPDGMLPVEIGGLKVLSVGFLLNEQDDPVIWRGPMKMGVIRQFIEDVDWGDLDLLV
                     VDVPPGTGDEPLSICQLIPELDGAVIVTTPQKVAAADVRKSINFCKELGVAVLGVVEN
                     MSGFACPKCGEVTPILSADGGRVTADEMGIPFLGSIPIDPLIAESGDNGKPFVESAAS
                     SPAAGAFREIVRVLTVTPTLSQGKPACV"
     gene            3731..6100
                     /locus_tag="PLZ74_07330"
     CDS             3731..6100
                     /locus_tag="PLZ74_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98170.1"
                     /translation="MTGLGLALLLVGGGQCADFYVATNGADRADGTREKPLATLAAAR
                     DAARRSGGGGHRIRLLPGEYFLEEPLTLDARDNGLTVEAEGAGRVTVYGGRRVTGWHR
                     DGEKFWCADLPEVKSGAWDFRALVVNGRMPDRARMPESGTFTHQNVFDAKWLSSVGGG
                     WERKPTHADLTTLVYHPTNLPPSLEVRNAELRVYHMWDDSLVGVASNDVARHTLHFTS
                     PAGHPAGAFGVKKYVVFNTREGMTRPGQWYLDRAAGRLVYWPLAGERMESASVIAPTL
                     TSVIRLRGSAQAPVERVTLRGFSVQATTTPLKPGGFSAHAYDGAVELYHAKECALIGM
                     EICHVGGQGIKSWALDACRIEACHIHHVGACGIRTGGDASVIEGNHIHHAGVYQPSAV
                     ALQFGRVRRDSKLSGFHIRRNEIHDAPYSGMTGGGSDHLIEENLIYRVMRELHDGAAI
                     YGSMSRTVLRGNVVRDVVEIGQGYGASAYYFDEGATGNVVERNVAIGVPMPTHNHITT
                     GVTIRDNVFVSDGDMKLSFPRSRDCVFTGNTLYAPGKVTVTAPNAVTSWVGNVLFSGN
                     AQGGSTPAAFAITDAMPPAPAPARRTWAVAVPRTEQPPVMDGEIGDGEWAGSFVNIDR
                     DPSRWLASGAPAFIKLMYDEQYLYVAANTVLFDIGLLSKGDRWGQDDGAELCIAGAKG
                     IAVIRGFAGGACVSVTDAGLPSEEAERLGKAVRFAAKPYGKTRNDWKSGWRGEWAIPF
                     KELGIRPEPGMKVAFNYGLFRAEDKVWRSLEGTGAANWRLSEAALLQFK"
     gene            6110..7015
                     /locus_tag="PLZ74_07335"
     CDS             6110..7015
                     /locus_tag="PLZ74_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOR98171.1"
                     /translation="MRRYRLTKAGFEVSRLAYGTWRLLADKPTAQEVNRRLHLCLELG
                     ITTIDTAEIYGKYGVEALLGEALALSPGLREQLEIVTKAGIYVPCEACPERRTAHYNA
                     TAEQLCASLDQSLKRLRTERIDLFLIHRPDWLTRTEDTAQGLNRLVQEGKIRAAGVSN
                     YAPAGVDLLNACLDRPLAVNQIAFSLLHMEAMEDGTLTQCERLGMTPMAWSPLAGGRL
                     FDPAEPAAGRLAAAAAACAVRYGGVTLDQFAYAWILAHPSHPMAIIGTNRLDRLRLAA
                     QADALTLEREDWYAFWEAAQGRRIP"
     gene            7034..7699
                     /locus_tag="PLZ74_07340"
     CDS             7034..7699
                     /locus_tag="PLZ74_07340"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR98172.1"
                     /translation="MERQTRRGGTTTGRSRTRNRILRAATKVFAEYGFREATTRMICA
                     EAAVNSALVNYHFRSKAELYRAVIVSLFEHIGKPMMRINETVTDEASWKAAVRTWIER
                     ALAICAASKPPEVWAARLMGMEECVPSELAQDIETKFAKPVRQCFQRLLRMGMKRDDP
                     VEIGLWASAIHAQCVVFALTRPGWVERFCPPGTSREDWLRRVTEHICEGVFSRLSYQG
                     ARK"
     gene            complement(7722..8384)
                     /locus_tag="PLZ74_07345"
     CDS             complement(7722..8384)
                     /locus_tag="PLZ74_07345"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR98173.1"
                     /translation="MKTQHLLFPVLLTALVTTADFSFPLFNGRDLSGWKVADMIGAGS
                     VTVLSDGTVECGFGTPMTGIAYTNAMPTLNYEIELEAMRVQGTDFFVALTLPVETNFC
                     TVIIGGWGGGLCGVSSVDYMDASENQWSEHVTFENNRWYRLRVRVTPGVLQVFLNDDL
                     YSARIEFDSSTRFSLRPGSDIDKTTPLGLATYCTRARWRHASLTRITTLTPADKPRSE
                     EP"
     gene            complement(8568..>9007)
                     /locus_tag="PLZ74_07350"
     CDS             complement(8568..>9007)
                     /locus_tag="PLZ74_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98174.1"
                     /translation="ADLLARIHASDAAVASGVVTEPVTNAYDTFPMLPDRDATRTPAD
                     SARLALHAERCAANQAWFYAAALLDASLRHPVWEGRTPSANRFMLARYHILAGQHAAA
                     RRNLLIALKQLDLPPTLGSSPVNDAPLMRFHLQRLLQALPSGR"
BASE COUNT         1804 a         2421 c         3073 g         1709 t
ORIGIN      
        1 atggtccacg cctgcctgta cgcggcggcc gagaactccg gcaagttggt ggcaacggtg
       61 cggcgcgagg cgcgccgtat cgccgaggcc aaagatttgc cgctgatcat caccgacggg
      121 ccgcccggta tcggttgccc ggtgatcgcg tcgctcaccg gcgcctccaa agtattggcg
      181 gtcaccgagc cgaccgtctc cggcgagcat gatctggagc gtgtgctgca actcacccga
      241 ttcttcgagg tgcctgtcgc ggtgtgcgtg aacaaatggg acatcaaccc tgatgtgacc
      301 gaaaggatcg agcgcaaagc tgaaaacctc ggcgccgcct gtattggccg cgtgcggtat
      361 gacaaagccg tgacaacggc gcagatccag cggaaatccg tggtggagat cgggtcggca
      421 gccggcgacg acatccgcca gatttgggaa aggatggagg gttcatggag ggagcgcgcg
      481 taaacgcgct acacaagacg gcaggcccgg cagtacccgc agcggttccg gcggtccgag
      541 acgtcccgct gtactggctg ggattggaga atgcgaaacc acaaaaaaag gagttacgat
      601 gaatacgaat aagatcaaga tcgcgatccc tctggcagag ggaatgctgt gcatgcactt
      661 cggacactgc gaaacgttcg cgctgttgga tgtggatttg acggggcgca agatcatccg
      721 ccgcgaggat gcggtgccgc cgccgcacga gccgggcgta ctgccgcgct ggctggcgga
      781 gcagaaggtg gagatggtta tggccggcgg catgggacag cgggcacagg ggcttttagc
      841 cgggcagaac atccgtgtga tcgtggggct ctcgcccgac acccctgaga atatcatcgc
      901 gcaatatctg gacggcacac tgaagtccgg cgagaatgtc tgcgaccact gaaaaaggct
      961 atgggccaaa aagtttccat caccatcttg gcggacaacc tcgcggctga agggctggcg
     1021 tctgaacatg gtttcgctgc ctggattgaa accgacggcg caaaaatcct gttcgacacc
     1081 ggcaacggcg atgccatgat gcagaatgcc gagacgttgg gtatcgcctt gccgcacgcc
     1141 gacatgatcg tattaagcca tgggcataat gatcacaccg gcaatctcgc tacgatcttg
     1201 cagcaagcgc cgcaagcgcg gctctatctg catccggagg ccttgcggca acggtacagc
     1261 atccgtgaga gtgccaagcc gataggcatg cccgcaagcg cggtgaatgc ggtctctggg
     1321 cttgcggagc acagacgtat gtgggtcacg gccaccgtgc agatttctga aaaagtccgg
     1381 ctgacaggac cggtgccgcg cagaaccgca tacgaggata cgggagggcc gtttttcaag
     1441 gatgccgcag gcgctcaccc cgacgacatt cccgacgata tgtcgctctg gatcgaaacc
     1501 cgcgcaggct tggtggtctg tctgggttgc tgccattccg gcgtgatcaa cacgctgaac
     1561 catatcacgg ctgtttccgg cgagcggcgg atccgttgtg tcgtgggcgg cacacacttg
     1621 gtgcacgctt ccgccgaaag aatgaagcag acaagcgcgg cattgaaagc ttacaatatc
     1681 ccgcgtgttg ttccttgcca ttgcaccggc ggggatgcgg tattttttct gaaagaagaa
     1741 tttggcgaaa cagtgcatca gggttacgcg ggtatgcgaa tcgatatctg atgcggcaaa
     1801 aaggatgata catgagcaag aagctgaaag tggttgtggt cggcgggctg gttgcctggc
     1861 cctacggaat cgtccgcggc gcgtgaggac actcgccctc caagaaccgc cgcaggtcgc
     1921 atgtcgcctg tcgcggtatg ggcagaaaaa gaactacgca aggaaagaag gtgtttatga
     1981 gtgacaacca aattattttt agaccgatcg gaataatccg taccgagcac acaaatccgg
     2041 aagccacgcc aatacaaccg atattcgcga aggactgcga gggatgtgtc atggttgccc
     2101 cggaattcgt ggcgggtctg gctgacatag aggatttctc gcatgtatat ctagtgtacc
     2161 atctacacaa agtaagtcgt gtgactctgg tcaccaaacc tttcatgcag gacaaagaac
     2221 acggcgtatt cgctacgcgt tcgccatgcc gcccgaaccc gatcggtttg agcatagtac
     2281 gacttgtcag ccgtgatggc ggcaccctga tcgtaaaagg agcggatatg ctcgacggta
     2341 cgccgctact ggacatcaag ccttacacgc ggcgttttga caatatttct actgagcgta
     2401 atggctggca ggacgaagtg gacgatgcca ctgccgcaaa acttggagtc cgtaactact
     2461 gcaatcgaca cttgtcatca gcagaatgaa accacaggaa aaccaaggca gaagagcttt
     2521 tagggacgtg gggttctgct agtccccttt tggactttga agcgacaaaa tatgaatttt
     2581 gaaaggagtt aaatgaaccc agaaaaaacg gcgtgtgatc aaagccaatg caacgcgtgc
     2641 agcgatgcgt cgtgtccctc tgcaggcagg caggcgcaac agccgcccat gcctgacgat
     2701 gccagactga agaaccgtct gagccggatc cggcgcaagg tggtcgtgat gtccggcaaa
     2761 ggcggcgtgg gaaagagcac ggtggcagtg aacttggcga tgtccgctat gctctcgggc
     2821 cagaaagtcg ggttgctgga catcgacctg cacgggccga gcgtaccggt catgctcggt
     2881 ctggtcggcc agcggctgga aggaggcccg gacggcatgt tgcccgtgga gatcggcggt
     2941 ctcaaggtgc tttcggtcgg ttttctgctg aacgagcagg atgatccggt aatctggcgc
     3001 ggccccatga aaatgggcgt gatccgccag ttcatcgagg atgttgattg gggcgacctt
     3061 gacctgctgg tggtggacgt gccgccgggc accggcgacg agccgctctc gatctgccaa
     3121 ctgataccgg aactggacgg cgcggtgatc gtgacgaccc cgcagaaagt ggcggcagcg
     3181 gacgtgcgca aatccatcaa tttctgtaaa gaacttggtg tggctgtgct gggggtggtt
     3241 gaaaacatga gtggtttcgc ctgcccgaaa tgcggcgagg tcacgccgat tttgagtgcg
     3301 gacggcggac gcgtgacagc cgatgagatg ggcataccgt tcctaggttc gatcccgatc
     3361 gatccgttga tcgcggaatc gggtgacaac ggcaagcctt tcgtcgaatc ggcggcgagt
     3421 tctccggcgg ccggtgcctt ccgcgagatc gtgagggtgt tgacggtcac tccgacgctg
     3481 tcacagggaa aaccggcatg tgtttaacct aaaaagagca gttgaacttt caactgctct
     3541 ttttaggttt ataggtttaa tgccttacgg tcgatcaggc caacgaaaac ggattgcgta
     3601 cggcaacggg atgtgtcacg ctaccctata cgttttcatg tcgcggcggg cgtccggttt
     3661 acgggtgcgc gtggaaagag aggatgcccc ggtttggcgg ggtaggggaa gggagttttg
     3721 cgtgaggacg atgacggggt tgggattggc gctcttgttg gtgggcgggg ggcagtgtgc
     3781 tgatttttat gtggcgacga acggggcgga ccgcgcggac gggacacggg agaagccgtt
     3841 ggctacgctg gccgcggcac gcgatgcggc acggcgttcc ggaggcggtg gacaccggat
     3901 ccggttgttg cccggtgagt attttcttga agaaccgctc acgctggatg cgcgggacaa
     3961 tgggctgacg gtggaagcgg agggggcggg ccgcgtgacg gtatatggcg gccgacgtgt
     4021 cacggggtgg caccgtgacg gcgagaagtt ctggtgtgcg gatctgccgg aggttaaaag
     4081 cggcgcgtgg gatttccggg cactggtggt gaatggccgg atgccggacc gggcgcggat
     4141 gccggaaagc ggcaccttca cccaccagaa cgtgttcgat gccaaatggc tctccagcgt
     4201 gggtggcggg tgggaacgca aacccacgca cgccgatctg accacgctcg tctatcatcc
     4261 caccaatctc cccccttcgc tggaggtgcg taacgcggag ttacgcgtgt atcacatgtg
     4321 ggatgattcg ctggtggggg tggccagcaa cgatgtcgcg cggcacacgt tgcatttcac
     4381 ctctccggcg ggccaccccg caggagcgtt cggcgtcaaa aaatacgtgg tcttcaacac
     4441 gcgtgagggc atgacgcgtc ccggacagtg gtatctcgac cgtgccgcgg gacgtctggt
     4501 gtactggccg ctggcgggcg agaggatgga gtccgcctcg gtcatcgcgc cgaccctgac
     4561 ctcggtgatc cgcctacggg ggagcgccca ggcgccggtc gagcgtgtga cgctgcgcgg
     4621 gttttcggtg caggcgacga ccacaccgct gaagcccggc ggcttttcgg ctcacgcgta
     4681 tgacggcgcg gtggaattgt atcatgcgaa ggagtgtgca ctcatcggga tggagatctg
     4741 ccatgtcggc ggccagggta tcaaatcctg ggcactggac gcgtgccgga tcgaagcgtg
     4801 ccacatccac catgtcggcg cctgcggcat tcgcacgggc ggtgacgcga gtgttatcga
     4861 gggcaaccac atccaccacg cgggggtcta ccagccgagt gcggtggcgt tgcagttcgg
     4921 gcgtgtgcgc cgggattcga agctgtcggg atttcatatc cgccgtaacg agattcacga
     4981 tgcgccgtac agcggcatga cgggcggcgg ctcggatcac ctgatcgagg aaaacctcat
     5041 ttaccgcgtg atgcgcgaac tacacgacgg tgcggcgatt tacggatcga tgagccgcac
     5101 ggtgctgcgc ggcaatgtcg tgcgcgatgt ggtggagatc gggcaggggt acggtgcttc
     5161 cgcttattat ttcgatgaag gggcgaccgg caacgtggtc gagcgcaacg tggcgatcgg
     5221 ggttccgatg ccgacccaca accacatcac gaccggcgtg acgattcggg acaatgtctt
     5281 cgtctcggac ggggatatga agttgtcgtt tccccgttct cgtgattgtg tcttcacggg
     5341 caatacgctg tacgcgccgg gcaaggttac ggtgacggca cccaacgcgg taacctcttg
     5401 ggtgggtaat gtgctctttt ccggaaacgc gcagggtggt tcaactccgg cggcctttgc
     5461 aatcaccgac gcgatgccgc ccgcgcccgc gcccgcccgc cggacttggg ccgtggcggt
     5521 gccgcgtacc gaacagccgc cggtgatgga cggtgaaatc ggtgacggcg aatgggcggg
     5581 gtcgttcgtg aatatcgacc gcgacccgtc acgctggctg gccagcggcg ctcccgcctt
     5641 catcaagctg atgtatgacg aacagtatct ctatgtggcc gcgaataccg tgctcttcga
     5701 tatcggcctg ttgagtaagg gcgatcgttg ggggcaggat gacggcgcgg aactctgcat
     5761 cgccggcgcg aagggtattg ccgtgatccg cggatttgcg ggcggcgcgt gtgtgagcgt
     5821 cacggatgcg ggtctgccgt cggaggaggc ggaacgtttg ggcaaggcgg tgcggtttgc
     5881 cgcgaaaccg tacgggaaga cgcggaatga ttggaaaagc ggctggcgcg gcgagtgggc
     5941 gatccccttc aaggagttgg ggattcgtcc ggaacccggt atgaaagtgg ctttcaatta
     6001 cggtctcttt cgcgcggaag acaaggtgtg gcggtcgctg gaaggcacgg gtgccgcgaa
     6061 ctggcgcctc agtgaggcgg cgctgttgca gttcaagtga gggggtgcga tgcggcggta
     6121 tcgtttgacg aaggcggggt tcgaggtctc gcggttggct tacggaacgt ggcgcttgtt
     6181 ggcggacaag ccgaccgcgc aggaggtcaa ccgtcgtctc catctctgtt tggaactggg
     6241 catcacgacg atcgacacag ccgagattta cgggaaatac ggggtggagg cgcttctcgg
     6301 tgaggcgctg gccctctcgc ccggattgcg ggagcagttg gagatcgtca ccaaggctgg
     6361 catttacgtg ccgtgcgagg cctgtcccga acggcggacg gcgcattaca acgccacggc
     6421 tgagcagctc tgcgccagtc tggaccagtc gctgaagcgg ttgcggaccg aacgcatcga
     6481 tctgttcctg atacaccgtc ccgattggct cacgcggaca gaagacacgg cgcaggggtt
     6541 gaaccgtctg gttcaggaag gaaagatccg cgccgcggga gtttcgaatt acgctccggc
     6601 aggggtcgat ctgctgaacg cttgtctgga ccggcctctg gcggtcaacc agatcgcctt
     6661 cagtctgctg cacatggagg cgatggaaga cggtacgttg acgcaatgcg agcggttggg
     6721 catgacgccc atggcgtgga gtccgttggc cggcggcagg ctgtttgatc cggctgaacc
     6781 ggcggccggt cgtttggctg cggcggctgc ggcatgtgcc gtgcgttacg gcggcgttac
     6841 gctggatcaa ttcgcgtacg cgtggattct ggcgcatccg agccatccga tggcgatcat
     6901 cggaaccaac cgtttggatc gcctgcgact tgcggcgcag gctgatgcgc tgacattgga
     6961 acgtgaagac tggtatgcgt tttgggaggc ggcacaggga cgtcgaatcc cctgaagcac
     7021 ggagggacgg aagatggaac ggcaaaccag acgaggcggg acaacgacgg gacgctcccg
     7081 cacacggaac cggattctgc gggcggccac aaaggttttc gccgagtatg ggtttcgtga
     7141 agccaccacg cggatgatct gcgcggaggc ggcggtgaac agcgcactgg tgaactacca
     7201 tttccggtcg aaagcggaac tctaccgtgc ggtgatcgtc tccctgttcg aacacatcgg
     7261 caaacccatg atgcggatca acgagacggt gacggatgag gcgtcctgga aagccgcggt
     7321 gcggacatgg atcgaacggg cgctggcgat ttgcgcggcc agcaagccgc cggaggtgtg
     7381 ggcggcgcgt ctgatgggga tggaggagtg cgtgccgtcg gagttggccc aggacatcga
     7441 aacaaaattc gcgaagccgg tgcgccagtg tttccaacgt ctattgcgca tgggcatgaa
     7501 acgggacgac ccggtagaga tcgggctttg ggcgagcgcc atccatgcgc agtgcgtggt
     7561 gttcgcattg acccggccgg gatgggttga acgtttctgt ccgcccggca ccagccgtga
     7621 agattggctg cggcgggtga cggaacacat ttgcgaaggg gtgttctcgc gtctctccta
     7681 ccaaggcgcg cgaaaatgag tgcgcgggaa atggtgccgc gttacggctc ctcgctgcgg
     7741 ggtttgtcgg caggggtcag tgtggtgatc cgcgtaagtg aggcgtgacg ccagcgggcg
     7801 cgggtgcaat acgtcgccag accgagcggg gtggtcttgt cgatgtcgct gccggggcgc
     7861 aggctgaagc gcgtggaaga gtcgaactcg atgcgcgcgg aatagaggtc gtcattcaga
     7921 aaaacttgca gcacaccggg cgtgacccga acgcgcaaac ggtaccagcg gttgttctca
     7981 aaggtgacgt gttccgacca ctgattctcc gaggcgtcca tataatccac ggaggagacg
     8041 ccgcagaggc cgccccccca gccgccgatg atcacggtgc agaagttggt ttccaccggc
     8101 agggtcagcg ccacaaagaa atctgtgccc tgaacccgca tcgcctccag ttcgatctca
     8161 taattcaggg tgggcattgc attggtgtag gcaatgccgg tcatgggagt gccgaaaccg
     8221 cactccacag tcccgtcgga cagaacggtc acgcttccgg ccccgatcat gtcggcgacc
     8281 ttccagccgg agagatcgcg gccgttgaag agcggaaagg aaaagtccgc tgtggtcaca
     8341 agtgcggtca gcaggacggg aaacagaagg tgttgtgttt tcatggtttc aggatagcgt
     8401 atacgatgac gaaaagcgat aacaacagat tgccggttac acgttacagg ttgtaagaac
     8461 gaggcgatgg ttaaaacttg cctatacatt gacaggattt cccgctgcgc gggcgccccc
     8521 gcccgccagt gagacacccg tcgcctgtct tttgtcctcc cccttcctta gcggccggac
     8581 ggtagggcct gcaacaaacg ttgcaggtgg aaacgcatga ggggcgcgtc gttgacgggg
     8641 gacgaaccga gagtcggcgg gaggtccagt tgtttcaacg cgatcagcag gttgcggcgc
     8701 gcggcagcgt gctggcccgc cagaatgtgg taccgggcga gcatgaaccg gttcgcgctg
     8761 ggagtgcgtc cctcccatac cgggtggcgg agggaggcgt cgagcaaggc cgcggcataa
     8821 aaccaagcct gattggccgc gcaccgttcg gcatgcagcg ccagtcgggc agagtctgcg
     8881 ggggtgcggg tggcgtcacg gtccggcaac atcgggaacg tgtcgtatgc gttcgtgacc
     8941 ggttcggtaa ccacgccgga cgcaaccgcc gcatcactgg catggatccg cgccagcaga
     9001 tccgcat
//
LOCUS       DAOSRB010000116         8919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_7464_length_8919_cov_6.806182, whole genome shotgun sequence.
ACCESSION   DAOSRB010000116 DAOSRB010000000
VERSION     DAOSRB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7464_length_8919_cov_6.806182"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..2430)
                     /locus_tag="PLZ74_07355"
     CDS             complement(343..2430)
                     /locus_tag="PLZ74_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98175.1"
                     /translation="MSENEKAKPAADGQENDEIVLDLNFAPNWARTSPEDQIQRYRND
                     RYDDDGEGHDRFGGRDRRPARFDGGDRDRRRPDRRPPPRRDRDERRGDFRGGGGMPRP
                     ASRGAVADLRGATSAAAPFEPRGPRPERRGPRTFRPVQPALPVDIRVLPEQKALGAVI
                     RRIQTSRRAFPLRDIAWLFLNNPNSCLVRIEPLKDQPVPLFQCKVCGMPALAEEDIRT
                     HLLNRHMEDFFDVEEVPCEPPTGVFVCVARCGMSGVLLGPPNHHSFNAKVQEMLRTRY
                     ANLSEEAYRARIEMVRDTEVVETWRQQCTRKKIYRRKGATPTVVEPVESATEDGDTET
                     PPEPEPKAPAMERETAELVFLREILPAQIASVRHLLCTASVAMQTSSRPLYYALKDIL
                     TRERRFPASLFFALRGAFRHRNLHLFRVNDAKGQDFVMFKPAVSLDPTHAVQALRDVL
                     TFVNEHPACTKAELATALAADDEAKLKETLVQLAWLVEKGHVIQFYNDVLSGPLEYPA
                     FRFLPGEKTAGGRQDGPRRPPSGTPASGDSAKDAPADAAAAVSDSTEETVSAPAVEKT
                     PASGDSAKDAPADAAAAVPDSTEETASAPAVEKTPASGDSAKDAPADAAAAVSDSTEE
                     TVSAPAVEKTPASGDSAKDAPADAAAAVSDSTEETVSAPAVEKTPASAEESVVPIAEA
                     STAADEPRTAPEA"
     gene            complement(2476..4425)
                     /locus_tag="PLZ74_07360"
     CDS             complement(2476..4425)
                     /locus_tag="PLZ74_07360"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOR98176.1"
                     /translation="MAQNVVEKILSAHRVSGELTPGGELAIRIDQTLTQDATGTMAFL
                     QFESMGLDRVKADLAVSYVDHNTIQVGFENADDHAYLKSVAQRYGAVFSRPGNGICHQ
                     LHLERFGKPGATLLGSDSHTPTGGGIGMIAIGAGGIDVAVAMGGGPFYLACPKVTRVW
                     LTGALKPGVSAKDVILKVLSVYGTQGNVGRMLEYGGPGVATLGVPARATLANMGAELG
                     VTTSVFPSDEVTRAFLKAQAREEDWCALEADPDACYESTLEIDLSTLEPLVACPHSPG
                     RIATVASLAGQRVNQVLIGSCTNSSYRDLKTVALMLRGRKTHPDVEVGLAAGSRQVVT
                     MLACEGLLAELIAAGVRILENACGFCIGNHMSPGTNAVSLRTSNRNFEGRSGTQSAQV
                     YLVSPETAAAAALTGVFTDPRSLETLPREVAEPSAFRIDDSMFLFRETAGRGVPGTEI
                     VRGPNIGKVPTGQPLPDRLDGMATIKVGDKITTDHIMPAGARLKYRSNVPQYARYVFE
                     HVSSSFYAEASAHRDAGLHNVIVAGESYGQGSSREHAAMCPMYLGVRALLAKSIERIH
                     FANLINFGIAPFLFDDPTAYDGISQGDRLVIDNLRTAIASDGRVTVRNVTRETTFTAT
                     AMLSTRQKNLLLNGGLLASTAGNAQ"
     gene            complement(4444..6177)
                     /locus_tag="PLZ74_07365"
     CDS             complement(4444..6177)
                     /locus_tag="PLZ74_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOR98177.1"
                     /translation="MNDENTAAGPDTGRHFLKQWIAEDVAAGRTGGQVVTRFPPEPNG
                     YLHIGHAKAICIDFGMAREFGGQCNLRMDDTNPSKESDEYVESIKRDIRWLGFDWGSG
                     FYSAADLFGQMYEIAENLIRSGHAYVCGLTQEEWKAYRGIPTAPGRESPTRNRSSEEN
                     LALFRRMRAGEFPDGSLCLRAKIDMASPNIHFRDPVLYRILHVPHYHAGDTWCIYPMY
                     DFAHPIEDAFEGVTHSMCTLEFEVHRPLYDWVIDRMDEMGLLVVRNGVKIRPQQREFA
                     RLNLTYTVMSKRKLLQLVEEKHVTGWDDPRMPTICGLRRRGYTPEAIRDFCERIGVSK
                     FESETDVALLEYCVRDDLNRRAVRRMAVLDPVKVVIDNLPEGHTAMVEVANNPEDPAA
                     GTRKIPFSREVYIERDDFMEVPAKKFFRMAPGQEVRLRAACLFTCTHVVKDDAGTITE
                     IHGTYDPDSTGGNAADGRKVKGTLHWVSVAHAVPLEVRLIDRLFTVPDPLGDPQRDFL
                     SFLNPDSCRTVTAYAEPALQEAEPGERFQFERLGYFCADTRDSRPGAPVFNRTVTLKD
                     SWARQTAGSKR"
     gene            complement(6229..6678)
                     /locus_tag="PLZ74_07370"
     CDS             complement(6229..6678)
                     /locus_tag="PLZ74_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOR98178.1"
                     /translation="MVAVLSGVALPLLIWWSGCLTDRIRPPSGSPSEVMVFEVTGYCD
                     CGICCNWERKWFGLGGAVVSSGPNKGQPKEIGVTASGTRTRHGTVAADTSVLPFGTIV
                     YVPGYGYGRVEDRGGAIKGAKLDLWYRSHAEAREWGRKTLRVRVWKP"
     gene            complement(6806..7036)
                     /locus_tag="PLZ74_07375"
     CDS             complement(6806..7036)
                     /locus_tag="PLZ74_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98179.1"
                     /translation="MTAALRDRMHRGEGTVLAEGVPAVVTLPSVAAKTRCYALDERGA
                     RKGDVPVERVAGGCRVAIGPQYKTVWYELDIR"
     gene            7406..>8919
                     /locus_tag="PLZ74_07380"
     CDS             7406..>8919
                     /locus_tag="PLZ74_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR98180.1"
                     /translation="MNDLTVLYLQNQPLAPDALQTLTHGLSDTFRAFCNETFPSEEPI
                     DWPPLRVVPVTSTQTLKQALFAVGPASGPLTEDGKNCVLFLLDDAFRDPASDQTIPLH
                     SLTLDGIPLTLWLYTYFPPIPKIVLTTPGTERVRVPSRRWVLKPREVFLQIHAHLPRV
                     RHLFRSLWEPRFWHALRHYVMDEAGSSWHTPGHNAGHAFSRSLFLQGFRHEYGAMTFR
                     ADLSVSVHSLGDLSTPGSRTPLADAQRLTSEIFGSAQSYYITNGSTTSNKAMLMTLLR
                     PGETVLLDRNCHKSVHHAVVMAGAIPNYLPARFNAHLGVWAPIAMEDLRHALTAHYPE
                     HAKPRMLVLTTCTYEGILYPVWEVARLCERYGILFYADEAWAPYLSFHPYYTAVTANG
                     RRVRYNAIHETSSAHFAVQSTHKTLAAFSQASMIHVSLRFKQLFESESTQWRWLLTRF
                     AVNGRGSYDKFIHDLHEVLRYWHSTSPHYPMMATLDCAGVQMRLEGLKLIEERLR"
BASE COUNT         1669 a         3036 c         2490 g         1724 t
ORIGIN      
        1 cgtcgcgggc cgagcgtcgc gggccgagca tcgcgggtcg agcgtcgcgg gccgagcgtc
       61 gcgggccgag cgtcgcgggt cgagcgtcgc gggccgagcg tcgcgggccg agcgtcgcgg
      121 gccgagcatc gcgggtcgag cgtcgcgggc cgaatgccgc gggccgcagg tcggtatcca
      181 cgttccgctt atccccctcc caggttcact gttcatccgg agcgaagttc cggatggtca
      241 gtttgtccac ttctcatgag aagtggacaa ggggacaacg tgaagaatgt gaacctgaaa
      301 ccggtttata cccacaccca cataagataa gtaaggcaag tcttacgctt ctggagccgt
      361 gcgcggttca tctgccgctg tcgaagcttc cgcgatggga acgacagact cttccgcaga
      421 cgcgggcgtc ttctcaactg cgggagcgga caccgtctcc tccgtgctgt ccgaaaccgc
      481 cgccgcagca tcggcaggcg cgtccttggc ggaatcgccg gacgcgggcg tcttctcaac
      541 ggcgggagcg gacaccgtct cctccgtgct gtccgaaacc gccgccgcag catcggcagg
      601 cgcgtccttg gcggagtcgc cggacgcggg cgtcttctca acggcgggag cggacgccgt
      661 ctcctctgta ctatccggaa ccgccgccgc agcatcggca ggcgcgtcct tggcggaatc
      721 gccggacgcg ggcgtcttct caacggcggg agcggacacc gtctcctccg tgctgtccga
      781 aaccgccgcc gcagcatcgg caggcgcgtc cttggcggag tcgccggatg cgggtgtgcc
      841 cgaaggcgga cgccgtgggc catcttgccg cccgccagcc gtcttttctc ccggcaggaa
      901 gcgaaaggcg ggatattcaa gaggaccgct cagcacgtcg ttgtagaact gaatcacatg
      961 ccctttctcc accagccacg cgagttgcac cagcgtctct ttgagtttcg cctcatcgtc
     1021 ggctgccagt gcagtggcga gttcggcctt ggtacaagcg ggatgctcgt tgacaaaggt
     1081 cagcacatcg cgcaacgcct gaaccgcgtg cgtaggatct agcgacaccg ccggtttgaa
     1141 catcacaaaa tcttgtccct tggcgtcatt gacgcggaac aagtgaagat tacgatgccg
     1201 gaaggcgccg cgcaacgcga aaaagagtga agccggaaaa cggcgttcgc gcgttaagat
     1261 gtccttcaga gcgtaataga gcggtcggct cgatgtctgc atcgcgaccg acgcggtaca
     1321 gagcagatga cggaccgacg cgatctgcgc gggcaaaatc tcacgcagga aaaccaactc
     1381 cgcggtttca cgctccatgg ccggcgcctt gggctcgggt tcgggcggtg tctccgtatc
     1441 cccgtcttct gtcgcggatt cgacaggctc gacaaccgtc ggtgtcgcac ctttacggcg
     1501 atagattttt ttacgggtgc actgctgccg ccatgtttcg acgacctcgg tatcacgcac
     1561 catctcgatg cgcgcacgat aggcttcctc cgacaggttt gcataccgcg tccgcaacat
     1621 ctcctgcacc ttggcattga aactgtgatg gttcgggggg ccgagcaata cgccgctcat
     1681 gccgcatcgc gccacgcata cgaatacacc ggtcggcggc tcacacggca cctcctccac
     1741 atcgaaaaaa tcctccatgt ggcggttgag cagatgcgtc cggatatcct cttccgccag
     1801 cgcgggcatg ccgcacacct tacactggaa taacggcacc ggctgatcct tgagcggttc
     1861 gatgcggacc agacaggagt tggggttgtt gagaaacagc caagcgatat cacgcagcgg
     1921 gaacgctcgg cgcgaggtct gaatgcgccg gatcacggcc cccagagcct tctgctccgg
     1981 caacacgcgg atatcgaccg gcaaggcggg ctgtacgggc cgaaacgtgc gcggaccgcg
     2041 ccgctcgggc cgcggccctc tcggctcgaa gggagccgca gcggacgtcg caccgcgcag
     2101 atccgccacc gcaccacgcg acgcgggccg cggcatgccg ccgccgcccc ggaagtcccc
     2161 acgcctctca tcacgatcac ggcgcggggg aggacgacga tcgggacggc gtcgatcccg
     2221 atcgccgccg tcaaagcgcg ccggacgacg gtcgcgcccg ccgaaacgat cgtggccctc
     2281 cccgtcatcg tcataccggt cgttccgata gcgctggatt tgatcctccg gagaggtacg
     2341 cgcccagttc ggcgcgaaat tcagatccaa cacaatctcg tcgttttcct gtccgtctgc
     2401 ggcaggcttc gccttttcgt tctcgctcat gatgcgtgga tgttaccttt tgtctgaccg
     2461 aatggtcaat ggatattatt gcgcgttgcc cgccgtggaa gcgagcagtc cgccgttcag
     2521 cagcagattc ttttgacgtg tactcaacat cgctgtagcg gtgaacgtgg tctcccgcgt
     2581 tacattgcgc accgtcaccc gtccgtcgga ggcaatggcc gtgcgcagat tgtcgatcac
     2641 caagcggtca ccctgcgaaa ttccgtcgta ggccgtcgga tcatcaaaaa gaaacggtgc
     2701 gatgccgaaa ttgatcagat tcgcaaaatg gatacgctcg atacttttgg ccagcagagc
     2761 ccgcaccccg agatacatcg ggcacatggc cgcatgctcg cgactcgatc cctgaccgta
     2821 actctccccc gccacgatga cgttgtgaag ccccgcatcg cgatgggcgc tggcctcagc
     2881 gtaaaaggac gaactgacgt gttcaaaaac gtaccgggcg tactgcggca cattggagcg
     2941 gtatttcaga cgcgcccccg caggcatgat gtggtcggtc gtgatcttgt cgcccacctt
     3001 gatcgtcgcc attccatcta accggtcagg cagcggctga ccggtcggca cctttccgat
     3061 attgggaccg cgcacgattt cggttcccgg gacccctcgg cctgcggttt cgcggaagag
     3121 gaacatgctg tcgtcgatcc ggaacgccga cggttccgcc acctcgcgcg gcagggtttc
     3181 gagacttcgc ggatctgtga acacgccggt caacgcggct gcggccgcag tctcagggct
     3241 caccaaatac acctgcgccg actgtgtccc gctacgtccc tcaaagttgc ggttgctggt
     3301 acggagactg acggcattcg tgcccggcga catgtggttt ccgatgcaga agccgcaggc
     3361 gttctccaga atacgcaccc cagcggcgat caactcggcc agcaacccct cgcatgccag
     3421 catcgtcacc acctgccgtg atcctgcggc caatccgacc tcgacatcag gatgcgtctt
     3481 gcgtccccgc aacatcaggg ccacggtctt caaatcccga taggaggagt tggtacatga
     3541 accgatcaga acctgattga cccgctggcc ggcaagcgac gcaacggtcg cgatgcgccc
     3601 tggcgaatgc ggacacgcaa ccagaggttc cagtgtgctc agatcgatct ccagcgtgct
     3661 ctcgtagcac gcatccgggt ctgcctccaa cgcgcaccaa tcctcctcgc gcgcctgtgc
     3721 cttcagaaaa gcccgcgtca cctcgtcgct tgggaatacc gaggtggtca cgcccaattc
     3781 cgcccccatg ttggcaaggg tcgcgcgcgc cggcacgccg agcgtggcca cacccggccc
     3841 gccatactcc aacatgcgcc ccacattgcc ttgtgtgccg tacaccgaca gaaccttcaa
     3901 gatcacgtct ttggccgaga cccccggctt gagtgcgccg gtcaaccaca cccgcgtcac
     3961 cttcgggcag gccagataaa acggtccgcc gcccatcgcc accgcaacat cgattccgcc
     4021 cgcgccaatc gcgatcatcc cgatcccgcc gcccgtgggc gtgtgcgaat cgctgcccag
     4081 caacgtcgcg cccggcttcc cgaaacgttc gagatgcaac tggtggcaga tcccgttacc
     4141 gggccgcgag aaaaccgcgc cgtatcgttg cgccacactc ttgaggtaag cgtgatcgtc
     4201 cgcattctca aaacccacct gaatcgtgtt gtggtccaca taactgaccg ccagatcggc
     4261 tttcacccgg tcaagcccca tgctttcgaa ttgcaggaac gccatggtgc cggtcgcatc
     4321 ttgcgtcagg gtctgatcaa tccgaatcgc cagttccccg cccggtgtca gttccccgga
     4381 cacccggtgt gcggacaaga tcttctccac cacattttgc gccatgaaaa accctttctc
     4441 ccgttagcgt ttcgatccgg ctgtctgccg ggcccatgag tctttgagcg tcacggtccg
     4501 gttgaaaacc ggcgcgccgg ggcgcgagtc gcgtgtgtcc gcacagaaat atccgagccg
     4561 ctcgaactga aagcgctctc ccggttccgc ctcctgtaac gccggttcgg catatgcggt
     4621 caccgtgcgg caggagtcag gattgaggaa cgagagaaaa tcacgctgcg gatcacccag
     4681 cggatccggc accgtaaaga gccggtcaat caagcgcacc tccagcggca cagcgtgcgc
     4741 gacggagacc caatggaggg tacccttgac tttccggccg tcggcggcgt tgccccccgt
     4801 cgagtcagga tcgtaggtcc cgtggatttc ggtgatcgtc ccggcatcgt ccttcaccac
     4861 atgcgtgcag gtaaacaggc aggcggcgcg caggcgcacc tcctgaccgg gagccatacg
     4921 gaaaaacttt ttcgcaggca cctccataaa atcgtcgcgc tcgatgtaca cctcgcggct
     4981 gaaggggatt ttgcgcgtgc cagcggcggg atcctcagga ttgttcgcca cctccaccat
     5041 cgctgtgtgc ccctccggca gattgtcgat caccaccttc acgggatcga gcaccgccat
     5101 acgccggacc gcgcgccggt tgagatcatc gcgcacacag tattccagca atgccacatc
     5161 cgtctcgctc tcgaatttcg acacgccgat gcgctcgcag aaatcacgga ttgcctcggg
     5221 ggtatagccc cggcgccgta agccgcagat tgtgggcatg cggggatcat cccaacccgt
     5281 gacgtgcttc tcctccacca actgcagcag cttcctttta ctcatcacgg tgtaggtcaa
     5341 gttcagccgc gcgaactccc gctgctgcgg acggatcttg accccgttgc gcaccaccag
     5401 caaccccatt tcgtccatcc ggtcaatcac ccagtcatac agcggacggt gcacttcgaa
     5461 ttcgagtgta cacatcgaat gggtcacccc ttcgaaggca tcctcgatgg gatgcgcgaa
     5521 atcgtacatc ggatagatgc accacgtgtc gccggcatga tagtgcggca catgcagaat
     5581 gcggtaaagc accggatcgc ggaaatgaat gttgggcgac gccatgtcga tcttggcgcg
     5641 caggcagagc gacccgtcgg gaaattctcc cgcccgcatg cgtcggaaca acgcgagatt
     5701 ctcttcagac gaacggttgc gggtcgggct ctcgcggccc ggagccgtcg ggatgccgcg
     5761 ataggccttc cactcctcct gcgtcagccc gcagacataa gcgtgaccgc tacggatcag
     5821 gttctcggcg atctcgtaca tctggccgaa cagatcggcc gcgctgtaga acccgcttcc
     5881 ccaatcaaaa cccagccaac ggatgtcccg cttgatgctc tcgacgtatt cgtccgactc
     5941 cttggagggg ttggtgtcgt ccatgcgcag gttgcactgt cccccgaact cccgcgccat
     6001 accgaagtcg atgcagatcg ccttggcgtg cccgatgtga aggtatccat tcggttccgg
     6061 cggaaaccgc gtcaccacct gcccccccgt ccgtccggct gccacatcct ccgcaatcca
     6121 ctgtttcaaa aagtgcctgc cggtatccgg ccccgccgcc gtgttctcgt cattcatatc
     6181 gtttctggtc tttggttaag cattcccttc accgggaagc ccgttttttt acggtttcca
     6241 taccctcaca cggagggtct tgcgacccca ctcccgagcc tccgcatgcg atctgtacca
     6301 caaatccagc ttggcacctt tgatcgcgcc tccgcggtcc tcaacgcgtc cgtaaccgta
     6361 gcccggaaca tacacgatgg ttccgaacgg cagcacggaa gtatccgccg ccacagtccc
     6421 gtgccgggta cgcgtcccgc tggccgtcac cccgatctcc ttgggctgcc ctttgttcgg
     6481 tccgctgctg accaccgcac ctcccaaacc gaaccacttc cgctcccaat tgcaacagat
     6541 gccgcagtcg cagtacccgg tcacctcaaa aaccatcact tcagacgggc ttccgctggg
     6601 aggacggatc cggtccgtga gacaccccga ccaccatatc aacaacggca gcgccacgcc
     6661 ggaaagtacg gcaaccacac gccatcgcca tcggtttgga tagtcccctg tgcacggttt
     6721 ggacataagg ggtactgtat caaatgcggc ccgtccccac aatgctgaac gctctcttgc
     6781 gcccgagtcc tttcattttt ccgacttaac gaatatcgag ttcgtaccac acggttttgt
     6841 actgcggccc aatcgcgaca cggcaaccgc ccgcaaccct ttcgaccgga acatcaccct
     6901 tgcgagcccc gcgctcgtcg agcgcgtaac agcgtgtttt cgctgcaaca gagggcagcg
     6961 tcacgacagc gggcacacct tcagccagca ccgtcccctc accccggtgc atgcggtcgc
     7021 gaagcgctgc cgtcatggat ctaccccctt ttcacatcat gctgcctcaa tccgttctcc
     7081 acaagcccgt tgaccgcatc acacgtgtca ggccatagcg tcagcgtccc gtccaaataa
     7141 ccgcacgggg catccccaac attacggatg tttacatcat atcgaacccc gccgttttcc
     7201 tccacccctt ttgcgtaaaa atgatctctg tcttgcgcaa acccggccca actcgcgttt
     7261 cgacgcccca tctacggcat gccggagttt gctgttgtca gccgtccgtt cttattttat
     7321 cctcttctcg tcacctagat tgggaaagga aaccagaaca ggcggagcgt ctcgacagcg
     7381 cggtaccccg tccgtcttag aagttatgaa tgacctgacg gttctctatc tccagaacca
     7441 gcctcttgcc ccggacgcgc tccaaacgct cacgcacgga ctttcggaca cgttccgtgc
     7501 cttctgcaac gagaccttcc cctccgagga accgatcgac tggccaccac tgcgggtcgt
     7561 gcccgtcacc agcacgcaga ccctgaaaca agcgctcttc gcggtgggtc cggcgagcgg
     7621 gccgctcact gaggacggta aaaactgcgt tctcttcctg ctggacgacg cgttccgcga
     7681 tccagcctcc gaccagacga ttccgctcca cagtttgacg ctagatggta tccccctcac
     7741 gctctggctc tatacctact ttccgccgat cccgaaaatc gtcctgacca cgccaggcac
     7801 agaacgtgtg cgggtcccct cgcggcgatg ggtgctcaaa ccccgcgagg tgtttctcca
     7861 aatccacgcc catctgccgc gtgtccgtca cctcttccgt tccctttggg aaccgcgctt
     7921 ctggcatgcc ctgcgccatt atgtgatgga cgaggccggc tcctcttggc atacgcccgg
     7981 ccacaacgcc ggccacgcct ttagccgctc gcttttcctg caggggttcc gccacgagta
     8041 cggggcgatg actttccgcg ccgacctcag tgtttcggtc cacagtctgg gcgacctctc
     8101 cacgccggga tcgcgcacgc cgctggccga tgcccaacgg cttacctcgg agatcttcgg
     8161 ctcggcccag agctattaca tcaccaacgg ctcgaccacc tccaataagg cgatgttgat
     8221 gacgctgctg cggcccggcg agaccgtgct gctggatcgc aactgccaca agtcggttca
     8281 ccatgccgtt gtgatggccg gtgccatccc caactatctg ccggcacgct tcaatgccca
     8341 tctcggcgtt tgggcgccga tcgccatgga ggatctgcgg catgccctta ccgcgcacta
     8401 tcccgaacac gccaagccgc gcatgctggt gctcaccacc tgcacctacg aagggatcct
     8461 ctatccggtc tgggaggtcg cgcggctttg cgagcgctac ggcattctct tttacgcgga
     8521 tgaggcgtgg gcgccttacc tcagcttcca tccctactac acggcggtga ccgcaaacgg
     8581 acgtcgcgtg cgttacaatg ccatccacga gacctccagt gcccattttg ccgtgcagtc
     8641 cacccacaaa acgctggctg ccttcagtca ggcctcgatg atccacgtct cgctgcggtt
     8701 caagcaactt ttcgagagcg aatcgaccca gtggcgctgg ctgctcacgc gcttcgccgt
     8761 caacggccgc ggctcttacg ataaatttat ccacgacctg cacgaggtgt tgcgctactg
     8821 gcactccacc tcgccccact atccgatgat ggctacgctc gattgcgccg gcgtgcagat
     8881 gcggctggaa ggcctcaagc tgattgaaga gcgcctgcg
//
LOCUS       DAOSRB010000117         8895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_7486_length_8895_cov_6.816855, whole genome shotgun sequence.
ACCESSION   DAOSRB010000117 DAOSRB010000000
VERSION     DAOSRB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7486_length_8895_cov_6.816855"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /gene="aroF"
                     /locus_tag="PLZ74_07385"
     CDS             <1..757
                     /gene="aroF"
                     /locus_tag="PLZ74_07385"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOR98181.1"
                     /translation="KVVFGGPQVVLIAGPCTVESRNQLLDIAGAVKEAGAVMLRGGAF
                     KPRTSPYTFQGLCAKGLEYLAEARAMTGLPFVTEVLETAHLHDVAQVADMVQLGSRNM
                     HNSVLLRAAGSCGKPVLLKRGMSARIEEYLLAAEYVLQGGARHVVLCERGIRTFETMT
                     RFSLDLNAVPLLKERTWLPVIVDPSHGTSVSRLVTPMARAAVACGADGLIVEVHNRPE
                     QALCDGKQSITLEAFAAMVKGVRDVAEAVGRTL"
     gene            848..2764
                     /locus_tag="PLZ74_07390"
     CDS             848..2764
                     /locus_tag="PLZ74_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOR98182.1"
                     /translation="MRAIKRIGLFVMLGGWVGAACAAECLSPEYLAVSPDGLTLYVTA
                     ATADKLLLVDPAAGRVKAEWTVRHETRRGFWGRLGFSTSSGAKRVAGLSGVAVAPDGT
                     VYVTGGGVNGFLIKLNAEGSVMGQVCLGHTPLAPVVAPDGATVYVPNRFSSNVAVVDV
                     ASMTVSATVPMLREPFAAALGKDGALLFVANHLPAVRATNDVVAASVSVIDTAQKTVV
                     RHLMLPNGSTGVRGICASPDGRFVYVTHTFGRYQLPTTQLERGWMNTAGLSVFDGVTG
                     DYVNTALLDDVDLGAANPWGVTVTSDGKTLVVAHAGTHELSLIDRPAFHARLEKAARN
                     EKVTEVTKSASDVPNDLSFLVTLRRRIRLPGNGPRGVVAVGDSVYAALYFTEELVAVK
                     LGGPVVKPVRVMLGASNAPPTDPVRRGEMFWNDATLCFQQWQSCTSCHPEGRMDGLNW
                     DLVNDGIGNPKQTKALVYCHLTPPTMVTGIRPDMESCNRKGLTHIQFVVRPEEDALCL
                     DAYVMSLKPEPSPYLLNGRLSPLAKKGEAIFKKAGCAECHPSAESGPQGEPLFTNLKK
                     YDLGLGVGNEEGRELDTPTLVETWRTAPYLYDGRALTMEEMLTVCNPKDTHGVTRALS
                     PEEIRALAEYVLSF"
     gene            2806..3840
                     /locus_tag="PLZ74_07395"
     CDS             2806..3840
                     /locus_tag="PLZ74_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOR98183.1"
                     /translation="MRKRHYGQGGVLRGQELGVFAPESGLPPAAKGVVLSQTGWGSRE
                     QILRAGVGVQGTEVPQLWLCGGDGQLPPVYQRLVVEGVSPRLIRRGACTVGVGYEDEH
                     ADYCLLTGVLPPDLNASREAQTTAVFEMIEDVLRDEGMTFAQVARTWLYMDRILDWYD
                     PFNRARDAFFHSRKVYDGLVPASTGIGSANLYGSAITACAIAVRPKRSGSLTLTALPS
                     PLQCAALEYGSSFSRAVEWLTPEARTVMVSGTASIEPGGATAFVGDVRRQIELTMDVV
                     RAILTSRGMDWADVTRAVMYLKRAEYQEEWEQYLREHALEGMPLITIEADVCRDDLLV
                     ELEADAVKRS"
     gene            3837..4604
                     /locus_tag="PLZ74_07400"
     CDS             3837..4604
                     /locus_tag="PLZ74_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOR98184.1"
                     /translation="MRAVLKKILSPGVAAWACWVAALGAVALAFPGATRAGALMARRP
                     MLMGTAVLAVWIGLAGIRALRGRRAASALLHLGCACVMAGWLVGRVAERSATPERPVT
                     GAMAMIAGDVSDKLWEGAYLTNFVGRLPFTVKLEQFFVERYTRNAVDIDAGREAPVRE
                     YRSRITVAEPGREPYVANVRVNQPLRVQGYRIYQMSWGQSQDLYGRPVTYTVLQFIRD
                     PGLPLVYAGFVLLFAGVLLFALRVGCARGTAAREVPS"
     gene            4601..5389
                     /gene="ccsA"
                     /locus_tag="PLZ74_07405"
     CDS             4601..5389
                     /gene="ccsA"
                     /locus_tag="PLZ74_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOR98185.1"
                     /translation="MMVKLTFQGGLIYLTLTLFVATALALRGGWRAIGRGFWFAGFIA
                     ALASVIWRGVHTGHPPMQNLFEFFLCMAAALWPLSQWSRWHNQLDTDLRDALLGVVIL
                     IPAGFVFSEEVRRLPPALQSPLFIPHVASYVAGYVMLARAALMALPLFRAATLDRMTL
                     ERTETAARQTAAVGFVLLTLGLLLGSLWGKICWGHYWQWDPKEMWSLATWLVYAGLFH
                     YRLRHGLRAPGTLAALLWLGLLFIVLTLTWINVSRLFVGMHNYA"
     gene            complement(5447..6799)
                     /locus_tag="PLZ74_07410"
     CDS             complement(5447..6799)
                     /locus_tag="PLZ74_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOR98186.1"
                     /translation="MNRRLFLKRAFAAGVSLTSRADAKPRAPRILLRSSWQVVNIGDI
                     AHTPGVLALIEKHLPGVEVILWASGDLTPEVAAMEHRRFPKLKIVKGRIGANGDASTP
                     ELAEALTWCDFLLHGSGPSLVAHKDVAAFVRHVRKPFGVYGITYAGGADPAQRDLMSQ
                     ARFLYFRDSVSLGRAREERIASPEMGFAPDGAFGCDLRNDAAAEAFLRANRLEPGRFL
                     CCIPRLRNTPYWKIRNRPMNAAAHAKHARNEALKEQDHAPLREAICAVTRTTGLRVLV
                     CPEDMSQMAVGREMLVNRLPEDVRRQVVWRESFWLTDEALSTYVRSAGLFGLEMHSPI
                     LCIGNGIPAIVCRFAEQTSKGFMWRDIGLDAWLFDFDQAADRARLPAAVLDMVQNPAD
                     TRARVAEARKRVEARQRETMAALADTLGYRGASGRTGVMIPSRMGSEERNVPGLAQVR
                     "
     gene            7284..8519
                     /locus_tag="PLZ74_07415"
     CDS             7284..8519
                     /locus_tag="PLZ74_07415"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOR98187.1"
                     /translation="MKKRRTGCFWGIMCGWVGVVISAGAQEQTAMICGAPVRQARAEI
                     LWTRVICKQPGRYIGWPSVCVRKNGEVLAVFSGDRDEHVCPFGKVQIVRSTDQGETWS
                     AATTICNSPLDDRDAGIIELPDGTLIVAWFTSLAYESSIRDRVALKPGSPLFYWWLHD
                     EKVLPEVKKTWLGYFTRRSSDGGKTWEAPVRTPGSAPHGPIVLKNGRLLYVGRTYTAT
                     SGIDDRGVSELSVAESRDQGRTWQRISAIPLPEGERIGAFHEPHAVETADGRLVVQIR
                     YQGKSGCLWQSESADNGVTWSVARQTGLAGLPPHLLRLRNGKLVSVYGRRFDPYGEYA
                     CLSDDHGRTWDLANEIKLAGAPGLVRLRPGGGGHRDGDLGYPASGELPDGSILTVYYQ
                     PEQEGEKPCLMATRWRVRE"
BASE COUNT         1553 a         2545 c         3073 g         1724 t
ORIGIN      
        1 caaggtggtc ttcggtggac cgcaagtggt tctgatcgcc ggcccgtgta cagtcgagag
       61 ccgcaaccag ttgctggaca tcgcgggcgc ggttaaagag gccggggccg tgatgcttcg
      121 cggcggggct ttcaaaccgc gcacctcacc ctatacgttt caggggctgt gcgccaaagg
      181 tctggagtat ctggccgagg cgcgcgccat gacgggtttg ccgttcgtaa ccgaggtgct
      241 ggaaacggcc cacctccatg atgtggcgca ggttgcggat atggtgcagt tgggatcccg
      301 caatatgcac aactcggttt tgctgcgggc ggccggaagc tgcggcaagc cggtgctgct
      361 gaagcgcggg atgagcgccc ggattgagga gtacctgctg gcggctgaat acgtgctgca
      421 gggcggtgcc cgccacgtgg tgctgtgcga acgcgggatc cggacattcg agaccatgac
      481 gcgcttctcg ctggatctga acgccgtgcc cctgctcaag gaacggacct ggctgccggt
      541 gatcgtggat cccagccatg gcacgagtgt gagccgtctg gtgacgccga tggcgcgcgc
      601 cgcagtggcc tgcggtgcgg acgggctgat cgtggaggtg cacaaccgtc cggaacaggc
      661 gctgtgtgac ggcaaacaga gcataacgct cgaagcattt gctgcgatgg tgaagggggt
      721 gcgcgatgtg gcggaggccg tgggccgcac gctttgactt tgaccctgcg ggcattgcgg
      781 tgtccgcgcg taaaagaaaa tccggaaggt cgtggatgaa ggtgtcaaac aacggaggag
      841 aacgggtatg agggccataa agcgaatcgg attgtttgtg atgttggggg gatgggtcgg
      901 tgcggcgtgt gcggcagagt gcctttcgcc ggaatatctg gcggtttctc cggatggcct
      961 cacgctgtat gtgaccgccg ccacggcgga caagttgctg ttggtcgatc cggcggccgg
     1021 acgggtcaag gcagagtgga cggtgcggca tgagacgcgc cgcggattct ggggccgcct
     1081 cggcttcagc acgtccagcg gagccaagcg cgtggcgggt ctgagcggag tggccgtggc
     1141 accggacggc accgtgtacg tcacgggcgg aggcgtgaac ggttttctga tcaaactcaa
     1201 tgcggaaggc agtgtgatgg ggcaggtctg tctggggcac accccgctcg cgccggtggt
     1261 tgcgccggat ggggcgacgg tgtatgtgcc caaccgtttc tcttccaacg tggcggtggt
     1321 ggatgtcgct tccatgaccg tttcagccac ggtgccaatg ttgcgcgaac cgttcgcggc
     1381 cgcgctcggc aaggacggcg ccctgctgtt tgtggccaac caccttccgg ccgtccgcgc
     1441 gaccaacgat gtggtggcgg cttcggtttc ggtgatcgac accgcgcaga agacggtggt
     1501 gaggcacctc atgttgccca acggctccac cggtgtgcgc ggaatctgcg cctcgcccga
     1561 tggccggttc gtctacgtca cccatacgtt cggacgctat cagttgccga ccacccagct
     1621 tgaacgcggc tggatgaata ccgctggcct cagcgtgttc gacggtgtga ccggcgacta
     1681 tgtcaacacc gcactgctgg atgatgtgga tctgggcgcg gccaatccgt ggggcgtcac
     1741 ggttacgtca gacggcaaga cgctggttgt ggcccatgcc ggaacgcacg agctgagcct
     1801 catcgaccgc cccgcgtttc atgcgcggtt ggaaaaggcc gcccggaacg agaaggtcac
     1861 cgaagtgacg aaatccgcgt cggacgtgcc gaacgatctc tcatttctcg tgacgctgcg
     1921 gcgcaggatc cggctgccgg gaaacggtcc ccgcggcgtg gtcgcggtgg gggacagcgt
     1981 gtatgccgcc ctgtacttca cggaagaact ggtcgccgtg aaactgggcg ggccggtggt
     2041 gaagcccgtg cgggtgatgc tgggtgcctc gaatgcgccg ccgacggatc ccgtgcgccg
     2101 cggcgagatg ttctggaacg atgccacact ctgtttccag cagtggcaga gctgtacgag
     2161 ctgtcatccg gaagggcgta tggacgggct gaactgggat ctggtgaacg acgggatcgg
     2221 caatccgaaa cagaccaaag ccctcgtgta ctgccacctc acgccgccca ccatggttac
     2281 cgggatccgt cccgacatgg agtcctgcaa tcgcaagggg ctgacgcata tccagttcgt
     2341 ggtgcgtcct gaagaggacg ccttgtgcct tgacgcgtat gtgatgtcgc tgaagccgga
     2401 gcccagtccc tatctcctca atgggcggct gtcaccgctg gcgaaaaaag gggaggcgat
     2461 cttcaagaag gcggggtgcg cggagtgtca tccgtccgca gaaagcggtc cgcagggtga
     2521 accgttgttc accaatctga agaagtatga tctcggattg ggtgtgggga acgaggaggg
     2581 acgcgagtta gacacgccca cgctggttga aacgtggcga accgcgccgt atctgtacga
     2641 cggacgcgcg ttgacgatgg aagagatgct caccgtgtgc aatccgaagg atacgcatgg
     2701 ggtcactcgg gcgctgtcgc ctgaagagat ccgcgcgctg gcggagtatg tcttatcgtt
     2761 ttaggtgaaa gggcgttgga gtacggcgcc agaggcggag gaagaatgcg gaaacggcat
     2821 tacgggcagg gtggggtgtt gcgggggcag gagttgggcg tgttcgcgcc ggagtcgggc
     2881 ttgccgcctg cggccaaggg tgttgttctg tcgcaaaccg gatggggaag ccgcgaacag
     2941 atcctccgtg ccggggttgg cgtccaaggc acggaggtgc cgcaactctg gttgtgcggg
     3001 ggggatggcc agttgcctcc ggtctaccag cggctggtcg tggaaggggt gtcgccgcgg
     3061 ttgattcggc gcggtgcctg caccgtgggt gtggggtatg aggatgagca cgcggactat
     3121 tgtttgctga ccggggtgct gccgccggat ttgaacgcat cgcgcgaggc gcagaccacc
     3181 gccgtgttcg aaatgatcga ggatgtgttg cgcgatgagg gaatgacctt cgcgcaggtg
     3241 gcccgaacct ggctctatat ggaccgtatc cttgactggt atgatccgtt caaccgcgcg
     3301 cgcgacgcgt ttttccactc acggaaggtg tatgacggtc tggtccccgc cagcacggga
     3361 atcggcagcg cgaacctcta cggttcggcg atcaccgcct gcgcgatcgc ggtccggccc
     3421 aaacgctccg gttcgctcac gcttacggcc ctgccctcgc ccctgcaatg cgccgcgctc
     3481 gaatacggca gttcgttcag ccgcgccgtg gagtggctca cacccgaagc ccggaccgtg
     3541 atggtctcgg gcacggccag catcgaaccc ggaggggcca cggcgtttgt cggtgacgtg
     3601 cggcgtcaga tcgaactcac gatggatgtg gtgcgcgcga tcctgacctc ccgcggcatg
     3661 gattgggcgg atgtcacgcg cgcggtcatg tacctgaaac gcgccgaata ccaagaagag
     3721 tgggagcagt accttcggga acatgcgctg gaggggatgc cgctgatcac cattgaggcc
     3781 gatgtctgcc gggatgatct gctggtggaa ttggaagcgg atgccgtgaa acgttcgtga
     3841 gagcggtctt aaaaaaaatc ctgtctcccg gcgtggcggc gtgggcctgc tgggttgcgg
     3901 cgctgggggc ggtcgctttg gcgtttcccg gcgcaacgcg cgcgggcgcg ttgatggcgc
     3961 ggcgccccat gctgatggga accgcggtgc tggccgtctg gatcggcctt gcggggatac
     4021 gggcgttgcg ggggcgccgc gcggcctcgg cattgctgca tctcggttgc gcgtgcgtga
     4081 tggccggttg gttggtcgga cgggtggcgg agcgttcggc aacaccggaa cggccggtca
     4141 cgggggccat ggcgatgatc gctggcgatg tctccgacaa gctgtgggag ggggcgtacc
     4201 tcaccaactt tgtggggcgt ctgccgttca ccgtgaagct cgaacagttt ttcgtcgagc
     4261 gctatacgcg caatgcggtg gatatcgatg cgggccgtga ggcaccggtg cgcgagtacc
     4321 gcagccgtat cacggtcgcc gagccgggcc gggagccgta cgtggccaac gtgcgtgtga
     4381 atcagccgct tcgtgtccag ggataccgca tctaccagat gagctggggg cagagtcagg
     4441 atctctacgg gcgtcccgtc acctacacgg tcttgcagtt catccgcgat cccggcctgc
     4501 cgctcgtcta tgccgggttt gttctgcttt tcgcgggagt gctgctcttt gcgttgcgcg
     4561 tcggctgcgc gcgcgggacg gcggcgcggg aggtgccgtc atgatggtga agctgacctt
     4621 tcagggcggt ctgatctacc tgaccctcac gctgttcgtg gcgaccgcgc tggcgttgcg
     4681 cggggggtgg cgcgcaatcg ggcgggggtt ctggttcgcg ggatttattg cggcgctggc
     4741 ttcggtcatc tggcgcggtg tgcatacggg ccatccgccg atgcagaatc tgttcgagtt
     4801 cttcctctgc atggccgccg cgctctggcc gctctcgcag tggagccggt ggcacaatca
     4861 actggacacc gacctgcggg acgcgctgtt gggcgtggtg atcctgatcc ctgccggctt
     4921 cgtcttcagc gaggaagtgc gccgtctacc tcccgcgctg cagagtccgc tcttcatccc
     4981 ccatgtggcg agttatgtcg cgggctatgt gatgctggcc cgtgcggcgc tgatggcgtt
     5041 gccgctcttt cgcgcggcta cgttggaccg gatgactctg gaacggaccg agaccgccgc
     5101 gcgccagact gcggccgtcg ggttcgtgct cctgacgctg gggttgttgc tgggttcgtt
     5161 gtggggcaag atctgctggg ggcattactg gcagtgggat cccaaggaga tgtggtcgct
     5221 ggccacgtgg ctggtatacg ccggcctctt ccattaccgc cttcggcacg gtctgcgcgc
     5281 gcccggtaca ctggcggcgc tgttgtggct cggcctgctg ttcattgtgc tgaccctgac
     5341 ctggatcaac gtgtcgcggc tttttgtcgg gatgcacaac tatgcgtgat tgcgtaacgt
     5401 gagttccggc gggtgcgggg acttcgcgtt tcaagttcaa agcgcctcat ctgacctgcg
     5461 caaggccggg gacgttccgt tcttcgctcc ccatgcggct cggtatcatg actcccgttc
     5521 gtcccgatgc gccgcggtat ccgagggtat cggcaagtgc cgccattgtt tcgcgctggc
     5581 gcgcttccac ccgtttgcgg gcctcggcca cgcgtgcgcg cgtgtcggcc ggattctgaa
     5641 ccatgtcgag cacagcggcg ggcaggcgcg cgcggtccgc cgcctgatcg aaatcgaaga
     5701 gccatgcgtc caaaccgatg tcgcgccaca tgaaaccctt gctcgtctgt tcggcgaagc
     5761 ggcagacgat cgcagggatg ccgttgccga tgcacaggat cggcgaatgc atctccaaac
     5821 cgaacagtcc ggcggaacgc acataggtgc tcagagcctc atccgtcagc cagaacgatt
     5881 cacgccaaac cacctgccgc cgcacatcct cgggaagacg gttcaccagc atctcccggc
     5941 ccaccgccat ttggctcatg tcctcggggc aaaccagcac acgcaggccg gttgtgcgcg
     6001 tgacggcgca gatcgcctcg cgcagcggcg cgtggtcctg ctccttcagc gcctcgttcc
     6061 gcgcgtgttt ggcgtgcgcg gccgcattca tcggccggtt gcgtattttc cagtacggcg
     6121 tgttgcgcag gcgcggaatg cagcagagga accggccggg ttcgaggcgg ttcgcgcgca
     6181 ggaacgcctc ggccgcggca tcgttgcgca ggtcacaccc gaacgcgccg tccggggcga
     6241 accccatttc cgggctggcg atgcgctctt cccgtgcgcg tcccagcgag acggagtcac
     6301 ggaagtatag gaagcgggcc tgactcatca ggtcgcgttg cgccggatcg gcgcctccgg
     6361 cgtaggtgat gccgtacacg ccgaagggtt tacgtacatg ccggacgaac gcggccacat
     6421 ccttgtgggc gaccagcgac gggccggaac cgtgcaacag gaaatcgcac cacgtcaggg
     6481 cttccgccag ttcaggcgtc gaggcgtcgc cgttcgcgcc gatgcggcct ttgacgatct
     6541 tgagcttcgg gaaacgccgg tgctccatgg ccgccacttc cggcgtgagg tctcccgaag
     6601 cccagaggat cacttcgaca ccgggaagat gtttctcgat caaagctagc acgccgggtg
     6661 tgtgggcgat gtcgccgata ttgacgacct gccacgagga gcgcagcagg atacgtggcg
     6721 cgcgcggttt cgcgtcggcg cgcgaagtca acgaaacccc ggcggcaaag gcgcgtttca
     6781 gaaaaaggcg gcggttcatg acgttcctcc tgaatggcgg gcagtgcggg tccgagccgt
     6841 gccgacacgt tactaaaaca gcgcctccgc ggcaaggcgt gagacgggga cggggaaacg
     6901 atcacgtctg tgtttcagag tacgcacgct aaacctaaaa agagcagttg aaccttcaac
     6961 cctctttggt ttcaaggttt cattcaaatc ggggtcggga tcgctatcgg gatcgggatc
     7021 gatcatttaa ccacggaata ccaccacctc acagcctcac agcctcacag tctcacagcc
     7081 tttgagattg cgggctccgc ccgcatctca atccgtcaaa acccaatgtt tacggggctt
     7141 tgctagaatt ttacccgttc aacggttcct tttgggctaa agtctggcca cggtcaacac
     7201 aacggatgcg ccggaagctg tggggcgctt gactacgaca gaggtgccga ctaaactacg
     7261 cgggataacg aaggggctcg acaatgaaaa aaagaagaac gggttgtttt tgggggataa
     7321 tgtgcggatg ggtcggtgtg gtgatatcgg ccggggcgca ggagcagacg gccatgatct
     7381 gcggcgcgcc ggtgcggcag gcgcgggccg agatcctctg gacccgggtg atctgcaaac
     7441 agccgggccg gtacatcggc tggccgtcgg tgtgcgtgcg taaaaacggc gaggtgctgg
     7501 cggttttttc aggcgaccgc gatgagcacg tttgtccctt cggcaaggtc cagatagtgc
     7561 gcagcacaga tcagggcgag acctggtctg ccgcaaccac gatctgcaac tcgccgctgg
     7621 atgaccgcga cgcggggatc attgagcttc ccgacgggac cttgatcgtg gcgtggttca
     7681 cttcgctggc ctatgagtcg agcattcggg atcgcgtggc gttgaaaccc ggctcgccgc
     7741 tcttctattg gtggctgcat gatgagaaag tgttgccgga ggtgaaaaag acgtggctcg
     7801 gttatttcac gcgccggtcc tccgatggcg gcaaaacgtg ggaggctccg gtacgcactc
     7861 ccggctccgc gccgcacggt ccgattgtgc tgaagaacgg tcggctgctg tatgtcggca
     7921 ggacgtatac cgcgacatca ggtattgacg accgcggggt gtcggagctt tccgtggcgg
     7981 aatcgcggga tcagggccgc acctggcaac gcatcagcgc gattccgctg cccgaaggcg
     8041 agcggatcgg ggcgtttcat gaaccgcacg cggtggagac cgcggacggg cggctggtgg
     8101 tgcagatccg ctatcaaggt aagagcggtt gtctctggca atccgaaagc gccgacaacg
     8161 gcgtcacatg gtccgtggcg cggcaaaccg gcttggccgg tctgccgcca catctgcttc
     8221 ggctgcgcaa cgggaaactg gtctcggtgt acggtcgccg tttcgatccg tatggggagt
     8281 acgcctgcct cagcgatgat catggccgca cgtgggacct cgcgaacgag atcaaacttg
     8341 cgggtgcgcc cggtctcgta cggttgcgtc ccggtggcgg cggtcaccgc gacggcgatc
     8401 tcggctaccc cgcttccggc gaactgccgg acggcagcat tctgacggtc tattaccagc
     8461 cggagcaaga gggggagaag ccctgcctga tggcgacgcg gtggcgtgtc agggagtgac
     8521 gtgaacggaa cgacaacggc tcggatgcgc gggtcgtaag ccgacgttca cgaaaaacgc
     8581 gaatcgtgta cgtgtacgag aacgagcacg aatacgggga aacgatcgcg gtcccgatat
     8641 ctttttccga catatatttc tcatgtacgc ctcacagaaa ccttattaac atccggcatg
     8701 atgacgccgt tccaagacaa gaaaggaaca ggtgaaaaca acacctttgc gtgaacagaa
     8761 aaggagcaga cagggaaggg atgcgtccgc aacgaatacg agaattgact cttttcaacc
     8821 cttcctgtat tctcgttttc aaatgtgtga gaacttgtaa cacgtgattc aaaagtgcat
     8881 gtcaagctaa tattt
//
LOCUS       DAOSRB010000118         8694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_7711_length_8694_cov_6.560250, whole genome shotgun sequence.
ACCESSION   DAOSRB010000118 DAOSRB010000000
VERSION     DAOSRB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7711_length_8694_cov_6.560250"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..5034)
                     /locus_tag="PLZ74_07420"
     CDS             complement(259..5034)
                     /locus_tag="PLZ74_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98188.1"
                     /translation="MRKRHCAMVLWCGLAGSVLANVSGTWIAPVGGLWSDTNNWQDGV
                     IPFGRGHTATFSAAAVSVTNDGARPVGVLKFNQTNIAIYGNAIVLDATGPKLIANNGT
                     AYLHVPVVATNPLTVTKETHTVSGVQLSRRNELEAGWVQVKSGVRLTSDARAMDPLPG
                     EDDAIRAPLSVRDQDLTLSAGGIFCLLGSAAAPTEQRFGVLNVKDYGGLDVVNGGFLA
                     PAELLVDDLAGPGLLSLSGGGRVAVASANRFVGTIRNPGADLRIEGTASPVLKPAADP
                     IMHLDASDLDSFTFAEENGTNFVVEWFSQVPGNYVRHDGHLTEGQRALPWVRTNVLNG
                     LPVVDFGNMIKTNKTERSLAPYLIWNTRSTAVKAAFAVLRTQNFIFTDTDKGHYHHSV
                     LSSGGMFRWDQTMLTSRGETAASFKNGEYVISLNGVPVTDPFSQKLSATEFDVVAMVV
                     KEGVTSVGTIAQDRSYRFGGQQVAEEIFYDRILTDAEIEATVAYLRQKWQGMEADAGM
                     AVAGPGALRVHVSDSNRSRLTVETGAAFAVETLSGSGIREVGGNGTLQVERGGSRPSR
                     PLALQGGTLALVNQGRAINTTLETAMDIPGLYFQLDASDLSSMTLDGDRVLEWRGNSN
                     VTNGLLAYAINTADNPAPRWVPGRLNGLPVVDFGAWGAGALLHWNHTNTAIQTLFMVF
                     DHIHPESFWLSDINDGNYANFHRGVNGLIFNTSYVTAGLQTGQVYLNGNRIDQMATFV
                     PEEEPFLISFVSTDGSAARAACMATDRYPRPGLLQYRSGGQRIAEVLVFNRHLNAAER
                     EQIEGYLMRKWLPNIPARFAASDGSQTFDGVVTSAADPVAVKVDTGQQAVLGALDGNG
                     PVEKTGAGTLVVGQVAGLTAPLSVKEGRLAFDTRVLPDPYVLPGGIAYHVDASDAASV
                     LLDADGVSVTNILDASGGSLSAQPSDPGIPPQLLPDALNGLPVISFRGAESGCAALWS
                     AKVSGIRTVFWVCGSQDGGGMPLGTASTAEGSDFKRTPILSAHSPIWAPESLAQHGVT
                     RIHGAVVNGRTTGFNGGYQLMSLVTDRGCSASAFATHKNVELFGGQRLAEVIIYQRLL
                     SDVERRDVEAYLARKWFGAPTSGYAGGRVDMNDLNMEGGTVDLPVGAAVSIRRFTGTA
                     DLLKNDDSVLSIADLSALSGSVVVSNGTFVLAGKPVQTELPVDGMLMHMDASVTGSFE
                     VVSENGTNFVTRWNSLVGSAYAAHDNISKRPWLLENELGGSPVVNFGPFKRQGWGDTA
                     ADGAYLIWDQPRLNIRSGFMVLGSQDGGNFLVGWNGSGGVADFHRGYGSGGYVDASGS
                     IIATGRTEPPLWIKSANAYWSVDGVRVNPGTTSLSGGYQAICFMSDPEHAPTNMLRGS
                     GFAIDRTFRWGGQRLAEFAIYDRLLTEEERLAVEVYLRNKWFGVLPTGYYQNGNVSNL
                     SVFAGGVVDVNGQARSVTHLGGNGTLSNGTLVVTGSLAPGAAEGQVGTLTADSLTLGD
                     GVVMRVDLAGGTCDRVFLNGMLTFGGAGTIVLSHTGTPTEGVVGDYMLFSASAIKTPE
                     FAEQWVVEGLPPGTGGKVCVSGTNVILSVFAQGTLLLMR"
     gene            complement(5164..5694)
                     /locus_tag="PLZ74_07425"
     CDS             complement(5164..5694)
                     /locus_tag="PLZ74_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOR98189.1"
                     /translation="MRKWMWVLWMPVLTSLAQDAGCPAAVQQEVKSYVWVKGDAAKQE
                     PVLREAAAFLEKAGHFYLATCEGDSPRVRPIKYVFIVDNKLLFVTSAKKGMYQQLLKN
                     PKVEISRTAVDKSAYLRFCGRAVLCGDAAVKAKLLEMEPVFGKKFGEDQAIFLVEPER
                     VGLFPMKGGEVRSKSF"
     gene            5887..7080
                     /locus_tag="PLZ74_07430"
     CDS             5887..7080
                     /locus_tag="PLZ74_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98190.1"
                     /translation="MAEKKKRVGMIGGGEASFMGPIHRAAIEQSGCVELVCGAFGSTR
                     QSSFETGRMLNLPPRRSYGTYRDLFRREASFPKTERIDFVTVLTPNAMHYPIAMSSID
                     ASFPIFSEKPFTCNLDEAVNLTRKLRNSGLAYGVAMVYPCYPALRKAQDLIQKDKAIG
                     VVRKVVATFSLGWMAQRLETAGNKQAGWRTDPRRSGPAGCLADLGSHAFYLSEWLTGL
                     SVSEVCADLRPTVAGRILDDDCTLLVRFETGARGVFLCSQIATGRQEGLAIEIYGDKA
                     ALGWSQSRPGRLTLTAIDGQREELTVNGSDEEEPLQTPPSPYGNNRAYIAALAECYRS
                     FAATLDRPKSQKTEPLPLYMSVEEGLRSVAFVDAVLQNTAVPEEDQPLPAKWMPVVVP
                     PVLDL"
     gene            7246..8547
                     /locus_tag="PLZ74_07435"
     CDS             7246..8547
                     /locus_tag="PLZ74_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HOR98191.1"
                     /translation="MTIQQGKTFTGFCLLSFCAAQGFAAGFGLYEASARGNAMGGALV
                     GATGDASANYFNAANLTELDGTHTMAGVTLVHPVLDITANGDKNNLDSGWFYPPHLYA
                     SQQLTEHWFTGFGVYTEYGLGTRYDRDWPLNWSSTKTTLESLTVNPNLAYKVNERLSL
                     GAGFRAMYLDFYHTKTPYQHDTIGVLPTPLPPLPVYGSMRTKVEGDSWGYGYNLGFSY
                     KITDTLDAGFVYRSRVRHTVKGDIEVQSSADTALPVPIPNPLPHGHASARGEIELPPS
                     WTAGLNYRPIDRLNLGFAATYTEWSSYDDLTITFGPTLANSDKLRRSSEEKNWNDVWR
                     LGIGADYMLTERWSVQCGYVYDMDPISKNNTDTMLPPGDRHIISTGLGYAVQTWTIHA
                     SYGLIVMKSCERTVTNGTKRAETDFDNGFCHLIALSVGKRF"
BASE COUNT         1811 a         2889 c         2349 g         1645 t
ORIGIN      
        1 gctttgctag aattctaccc gttcaactgc tccttttggg ttcaatcctg aaaattctgg
       61 atacatgcgc ccgcgcagcg ggaaatcctg tcagttccag cacatatcct tgagcccagg
      121 ccttcatccc ccctccttca cgttccgtgg catcaggttt actttaccca agccttcagg
      181 tgtcgggccg gcccgtccgg accggcccga ccgacatctc ctccgctgtg cgcaaagcgg
      241 cgttgctgcg ggcgacgttc accgcatcag gagaagcgtg ccctgcgcaa agaccgacaa
      301 gatcacattg gtgccggata cgcatacctt cccgccggtt cccggcggca gcccttcgac
      361 aacccactgt tccgcgaatt caggcgtttt gattgcagag gcggagaaaa gcatgtagtc
      421 gccgaccaca ccctcggtgg gcgtgcccgt gtgcgacagg acaattgtgc cggctccgcc
      481 aaaggtcagc atgccgttca ggaagacccg gtcgcatgtg ccgcccgcaa ggtccacacg
      541 catcaccacg ccatcgccca acgtcagact gtccgccgtg agggtcccca cctgaccctc
      601 cgccgctccc ggcgccagag aaccggtcac aaccagcgtt ccgttgctga gggtcccatt
      661 gcctccaagg tgcgtcaccg aacgcgcctg tccattaaca tccaccacgc caccggcaaa
      721 gaccgacagg ttcgagacgt ttccgttctg ataataaccg gtcggcagca caccgaacca
      781 cttgttacgc aggtaaacct cgaccgccaa ccgctcctcc tctgtcagca agcggtcata
      841 gatcgcgaat tcggcaagcc gctgtccgcc ccagcggaag gtccggtcga tcgcgaagcc
      901 actgccgcgc aacatgttgg tgggcgcgtg ttccggatca ctcatgaaac agatggcctg
      961 ataccctccg gagagcgagg tcgtgcccgg attcacacga accccgtcga cgctccaata
     1021 ggcgttggcg gatttgatcc acagcggggg ctccgtacga cccgtggcga taatcgaacc
     1081 gctggcgtcc acatacccac cggatccgta cccgcggtgg aaatccgcga cgccgcccga
     1141 gccgttccat ccgaccagaa agttcccgcc gtcctgggat ccgagaacca tgaacccgct
     1201 gcggatgttt agccgcggct gatcccaaat cagatacgcg ccgtctgccg cggtgtcccc
     1261 ccatccctga cgtttgaagg ggccgaagtt caccaccggc gaaccgccca gctcgttttc
     1321 aagcagccaa ggccgtttcg aaatgttgtc atgcgccgcg tacgcagacc cgaccagact
     1381 gttccagcgg gtgacgaaat tcgtcccgtt ttccgaaacg acttcgaaac tgccggtcac
     1441 actcgcgtcc atgtgcatca gcatcccgtc caccggaagt tcggtctgca ccggtttgcc
     1501 ggccaggaca aacgttccgt tggaaaccac aaccgacccg gaaagcgcac tgagatcggc
     1561 gatcgaaaga acgctgtcgt cgttcttcag aagatcggcc gtacccgtga accgccggat
     1621 cgatactgcc gccccgacgg gcaggtcgac cgtcccgccc tccatgttca gatcgttcat
     1681 gtccaccctg cccccggcgt aacccgaggt cggcgcacca aaccattttc gagccagata
     1741 cgcttcgacg tcgcggcgct ccacgtcaga aagcaggcgt tgataaataa tcacctctgc
     1801 cagccgctgt cctccgaaaa gctccacgtt tttatgcgtg gcaaaggcgg aagcgctgca
     1861 cccgcgatcc gtcaccaaac tcatcaactg atatccgccg ttgaatccgg tcgtacgtcc
     1921 gttcacaacc gcgccgtgga ttcgcgtcac accgtgctgc gccaacgatt caggagccca
     1981 aatcggcgaa tgcgcggaga gaatgggagt ccgtttgaag tcgctgcctt ccgcagtcga
     2041 agccgttccg agcggcatcc ccccgccatc ctgcgacccg cacacccaaa acacggtccg
     2101 gatgccgctc actttcgcac tccacaaagc cgcgcagccg ctttccgcgc cccggaagga
     2161 gatcacgggc agcccgttca acgcatccgg cagcaattgc ggcggaattc ccggatccga
     2221 cggctgggcg gagaggctgc cgcctgacgc gtccagaatg ttggtgaccg ataccccgtc
     2281 cgcatccagc agaaccgatg ccgcatccga ggcatcaaca tggtacgcga taccgccggg
     2341 caaaacatac ggatcaggca gaacgcgcgt gtcaaaagcc agccgcccct ctttcacgct
     2401 caacggcgcc gtcaaccccg cgacctgtcc caccaccagt gtgcccgcac cggttttctc
     2461 gaccggtccg ttcccgtcca aggcgcccag caccgcctgt tggcctgtgt ccaccttcac
     2521 ggccaccggg tcagccgcgg aggtgaccac cccgtcaaag gtttgcgaac cgtcggacgc
     2581 ggcaaaacgg gcgggtatgt tcggcagcca cttgcgcatc agatacccct cgatctgctc
     2641 gcgttcggca gcgtttaagt gccggttaaa gaccagcact tcggcgatcc gttgtccgcc
     2701 gctgcggtat tgcagcaggc ccggccgcgg ataccgatcc gtcgccatgc aggcggcccg
     2761 cgcggcagaa ccgtcggtac tcacgaagga aatcaggaac ggctcctctt ccggcacgaa
     2821 cgtggccatc tgatcgatcc ggttcccgtt caggtaaacc tgaccggttt gcaacccggc
     2881 cgtcacataa gacgtattga aaatgagtcc gttcacaccg cggtggaagt tcgcgtagtt
     2941 gccgtcattg atgtcggaca gccaaaaact ttccggatga atatggtcga acaccatgaa
     3001 cagcgtctgg atcgcggtgt tcgtatgatt ccagtgaagc agtgcgcctg caccccacgc
     3061 accgaaatca accaccggca gcccgttcag tcgccccggc acccatcgcg gcgcgggatt
     3121 gtcggcggtg ttgatggcat aggccagcaa accgttggtg acgttgctgt ttccgcgcca
     3181 ctccaaaacc cggtcgccgt ccagagtcat tgaggagagg tcgctcgcat ccagttggaa
     3241 atagagaccg ggaatgtcca tcgctgtttc cagtgtggtg ttgattgcgc gtccctggtt
     3301 gaccaacgcc agcgtgccgc cctgcagtgc cagcggacgg gaaggccgcg aaccgccgcg
     3361 ctcgacctgc aacgtgccgt tgccgccgac ctcgcggatt cccgaaccgc tcagcgtctc
     3421 cacagcaaag gctgcgcccg tctccacggt cagacgcgac cggttactgt ccgagacatg
     3481 cacgcgcagg gcgccgggtc cggccaccgc catccctgca tccgcttcca tcccctgcca
     3541 tttttgccgc agataggcga cggtcgcctc gatctccgca tccgtcagga tacggtcata
     3601 aaagatctcc tccgccacct gctggccacc gaaacggtac gaacggtcct gagcgatcgt
     3661 ccccacggat gtgaccccct ctttcaccac catggcgacc acatcgaatt ccgtggcgct
     3721 caacttctgc gaaaaggggt cggtcaccgg aacgccgttc agcgagatca catattcccc
     3781 gtttttaaag gacgcggcag tttccccccg ggaagtcagc atagtctgat cccaacggaa
     3841 catccctccg gatgacagca cagaatggtg gtagtgtccc ttatccgtat cggtgaaaat
     3901 gaaattctgg gtacggagca cagcgaacgc ggccttgacc gccgtggatc gcgtattcca
     3961 gatcagatac ggcgccagcg accgctctgt tttattcgtc ttgatcatgt tcccgaagtc
     4021 gaccaccggc aacccattca gcacattcgt cctcacccaa ggcaaggcgc gctgaccttc
     4081 cgtcagatgc ccgtcatgcc gaacataatt acccggcact tgcgaaaacc actcgaccac
     4141 aaaattcgtg ccgttctctt ccgcaaaggt gaacgaatcc aaatccgaag catcaagatg
     4201 catgatggga tccgcggcag gcttcagcac cggcgacgcg gttccttcaa tccggagatc
     4261 cgcaccggga ttgcgaatcg tcccgacaaa gcggttggct gaagcgaccg ccacacgccc
     4321 gcctcccgaa agggaaagga ggcccgggcc cgccaaatcg tccacaagaa gttccgcagg
     4381 agccaaaaat cctccgttca cgacatcgag ccccccgtag tcttttacgt tcagcacccc
     4441 gaagcgttgt tcggtcggcg cggcggccga cccgagcaga cagaagattc cgcctgccga
     4501 aagggtcaga tcctgatccc ggacagacag cggggcgcgg atcgcatcgt cctcgccggg
     4561 caacgggtcc atcgcgcggg catcggacgt aagcctcaca ccggatttga cctgcaccca
     4621 tccggcctcc agctcattac gccgcgagag ttgaacgcca gacacggtgt gtgtctcctt
     4681 cgtcacggtc aatgggttgg tggccaccac cgggacatgc agatacgccg tcccgttatt
     4741 ggcgatcaac ttgggcccgg tcgcgtccag cacgatcgcg ttcccgtata tcgcaatatt
     4801 cgtctgattg aatttcagga cgccgaccgg gcgcgcgcca tcgttcgtga cgctcaccgc
     4861 cgcagcagaa aaggtcgccg tatgaccgcg cccaaagggg atcacgccat cctgccagtt
     4921 gttcgtgtcg ctccaaagac cgccgaccgg cgcgatccac gtcccgctga cattcgccag
     4981 aacggaaccc gcaagcccgc accacaagac catcgcacaa tgccgttttc tcatcacacc
     5041 ctcctcttat cctccgaacg cccgttcatt ttggaaacac atatgccaca cggcacccgg
     5101 cggcgcggcg aatacgcccg cattaccgga tggcctgtcg tccaatcccg ctgcggaaag
     5161 cggtcagaag cttttgcttc ggacctcgcc gcccttcatc gggaagagac cgaccctttc
     5221 gggttccacc agaaagatcg cctgatcctc accgaacttc ttgccgaaga ccggctccat
     5281 ctcaagcaat ttggctttaa ccgccgcgtc accgcacagc acggccctgc cgcagaaacg
     5341 tagatacgcg ctcttgtcca ccgccgtgcg cgagatttcc accttcgggt ttttcagaag
     5401 ttgctgatac atccccttct tggcggaggt cacgaagagc agcttgttgt ccacaatgaa
     5461 cacgtatttg atgggacgca ctcgcgggct gtcgccctca caggtcgcca gataaaagtg
     5521 gcctgctttt tccaagaaag ccgccgcctc gcgcagcacc ggctcctgct tggccgcgtc
     5581 ccccttcacc cacacgtaac ttttcacctc ttgttgcacg gccgcagggc aacccgcatc
     5641 ctgcgccaga ctggtcagca ccggcatcca cagcacccac atccatttac gcatacggcc
     5701 tttcctttct ttgctgtctg acacgacacc attaaaccat gaaacattcc gcagtttact
     5761 tgaattccac gagaaaagaa acacgaaaac acgcattcat ttttgattgg cgaaaattca
     5821 gccaatccgg tattatcctt cataacatca tggttggcat gcgctggaaa gggaatgggt
     5881 gaagacatgg cggagaaaaa gaaacgggtg ggaatgatcg gcggcggcga ggcttctttt
     5941 atgggcccca tccaccgcgc cgcgattgaa cagagcggct gcgtcgaatt ggtctgcgga
     6001 gcctttgggt ccacgcggca gagttcgttc gagacgggac ggatgctgaa tctgccgccc
     6061 cgccgttcct acgggactta ccgcgacctc ttccggcgcg aagcttcgtt ccccaaaacg
     6121 gaacgcatcg attttgtcac cgtgctcacg ccgaacgcaa tgcactatcc gatcgccatg
     6181 tcctcgatcg acgcttcgtt cccgattttc agcgagaaac ccttcacctg taatctggat
     6241 gaagcggtca acctgacccg gaaactgcgg aacagcggtc tcgcctatgg agtggcgatg
     6301 gtctatccgt gttatcccgc cttgcgcaaa gcgcaggatc tgatccaaaa agacaaggcg
     6361 atcggggtcg tgcgcaaagt cgtggccacc ttttcattgg ggtggatggc ccaacggctg
     6421 gaaaccgcag gcaacaaaca ggccggctgg cggactgacc cgcgccggag cggaccggcc
     6481 ggctgtctgg cggatctcgg atcgcacgcg ttttatctct ccgaatggct aaccggcctc
     6541 agcgtgagcg aggtctgcgc cgatttgcga cccacggtcg ccggacgcat cctcgatgac
     6601 gactgcacgc ttctggtgcg cttcgagacc ggcgcgcgcg gcgtattcct gtgcagtcag
     6661 atcgccaccg gcaggcaaga gggcctcgcg atcgaaatct acggggataa ggccgcgctc
     6721 ggttggtcgc agagccgccc cggacggctg acgctcaccg cgattgacgg gcaacgcgaa
     6781 gaactcaccg tcaacgggtc ggatgaggag gagcccttgc aaacaccccc ttcgccctac
     6841 ggcaacaacc gcgcctatat cgccgcactg gcggagtgtt accgctcttt cgccgcgacg
     6901 ctggatcgcc ccaaaagcca aaaaacggaa ccgcttcccc tctacatgag tgttgaagaa
     6961 gggttgcgtt cagtcgcgtt tgtcgatgcg gtgctccaga acaccgccgt tccggaagag
     7021 gatcagccgc tgccagccaa atggatgccg gtggtggtcc cccctgtact ggacctgtaa
     7081 cgcgcggaaa cgccgcgcac agaacattgc cggtctccct tcacgaacaa acaaaccacg
     7141 ccatattcgc ttgtcttccg tgacaaaaaa acatattgtg tcaaagtgtt atttcaaacg
     7201 ggacggcttg tgcgtggatt cgcgcatcaa cacgaggggt gcaacatgac catacagcag
     7261 ggaaaaacgt tcaccgggtt ctgtttgctt tcattctgtg ccgcacaggg atttgccgcc
     7321 gggttcggcc tctatgaggc cagcgcccgc ggcaacgcca tgggcggcgc gctggtcggc
     7381 gccaccggcg acgcctcggc caactatttc aacgcggcca atctgacgga actggatggc
     7441 acgcacacga tggcgggtgt gacgttggtg cacccggttc tggacatcac tgcgaacggg
     7501 gacaagaaca atctcgacag cggctggttt tatccgccgc acctttacgc ctcccagcaa
     7561 ctcacggagc actggttcac cggtttcggc gtctacaccg aatacggctt gggcacgcgc
     7621 tatgaccgcg actggccgct caactggagc agtaccaaaa ccacgctgga aagcctgacc
     7681 gtcaacccca acctcgctta caaggtgaac gagcgcctct cgctcggcgc cggattccgt
     7741 gcgatgtatc tggatttcta ccataccaag acgccgtatc agcacgatac gatcggtgtc
     7801 ctgcccacgc ctctgccgcc gctgcccgtt tacgggtcga tgcgcaccaa agtggaaggc
     7861 gattcttggg ggtacggcta caatttaggg ttttcctaca aaatcaccga cacactggac
     7921 gccggtttcg tttaccggtc tcgcgtccgg cacacggtga agggcgacat cgaagtccaa
     7981 agctccgccg ataccgcgct gccggtaccg atccccaatc cgcttcccca cggccatgcc
     8041 tcggcacgcg gcgagatcga actgccgccc tcgtggaccg ccggcctgaa ctaccggccg
     8101 atcgaccggc tcaacctcgg attcgccgcg acctataccg agtggagttc gtatgatgac
     8161 ctcaccataa ccttcggccc cactctggcg aacagcgaca agctgcgccg ctcctccgag
     8221 gaaaagaact ggaatgacgt ctggcgtctg ggaatcggtg ccgactacat gctgacagaa
     8281 cgctggtccg tccagtgcgg ctatgtctac gacatggacc ccatcagcaa gaataacacc
     8341 gacaccatgc tgccccccgg cgaccgccac atcatcagca ccggcttggg ttatgccgtg
     8401 caaacctgga cgatacatgc gagctacggg ctgattgtga tgaaatcatg cgaacgaacc
     8461 gtcacgaacg gcaccaaacg cgcggagacg gatttcgata atggcttttg ccatctgatc
     8521 gccctctcgg tcggcaagcg cttctgatcc actcaggcgc ttacctaacg caggcgaagc
     8581 ccgcaaccca attcaattag ttaattagtt aattagttaa ttcgtgcatt cgttttccat
     8641 gaccatccgc agtcggttcg tggccgggat gtgcgaagat catgcgtttc tggt
//
LOCUS       DAOSRB010000119         8632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_7782_length_8632_cov_6.792818, whole genome shotgun sequence.
ACCESSION   DAOSRB010000119 DAOSRB010000000
VERSION     DAOSRB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7782_length_8632_cov_6.792818"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /gene="tig"
                     /locus_tag="PLZ74_07440"
     CDS             <1..1114
                     /gene="tig"
                     /locus_tag="PLZ74_07440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOR98192.1"
                     /translation="EIQEEVRSRLFRSLYKPALEQKKIAFVALRDVGDMLFSPETGIS
                     FSMTVDVKPEFELPRYKKIPVVFEEPVVTEEQVDGQIQQLREAFAQFKEAGADDEIRD
                     GDMVRIDFAGTVDGQSLAELDPAAKALAGESGQWVLVQEGRFLPEILQALRGLKQGAE
                     TTAQVTFGADHPITALQGKTATYSVTVREIRARSLPADEELCGRLKVASIDEMRTQTR
                     EGMMEAARKSERIRREQTVIDFLLKQADFDLPESEEAEEINTILDQMMGEAQSRGLTR
                     EDLEKSRGQIIENATTTARRQLRLRYILARIAEKEALTLSPEEIDSRIAEFANASRST
                     PAQFRAQVEKNGRMDALREHMLNLKTIQFLLDQAKG"
     gene            1127..1738
                     /gene="clpP"
                     /locus_tag="PLZ74_07445"
     CDS             1127..1738
                     /gene="clpP"
                     /locus_tag="PLZ74_07445"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOR98193.1"
                     /translation="MESPQANMLIPIVVEQTGRGERAYDIYSRLLQDRIIFIGTAITD
                     EVSNVIIAQLLFLQAQDATKEISVYINSPGGSVTAGLAIYDTMQFISCDVATYCVGQA
                     ASMGAVLLAAGSKGKRFSLPNARIMIHQPWGGAEGKATDISIAAKEILRLKDRLNEIL
                     AHHTGKSVDVLSNDTERDYFMSADEAKTYGLVDDVLTQSLAEK"
     gene            1742..2992
                     /gene="clpX"
                     /locus_tag="PLZ74_07450"
     CDS             1742..2992
                     /gene="clpX"
                     /locus_tag="PLZ74_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOR98194.1"
                     /translation="MAKRPPKKAMLTCSFCGRGDRADAVVIPGPDVNICSDCVDICTR
                     ILAENNATPASSAALPVLHVPPPGEIKTFLDQYIVGHDRTKKILAVAVHNHYKRLNQP
                     LTKKDAFADVEIEKSNILLIGPTGCGKTLFARSLARMLDVPFAIADATTLTEAGYVGE
                     DVENILLYLLQAADMNVARAERGIVYIDEIDKIARKTENVSITRDVSGEGVQQALLKI
                     IEGTVSRVPPQGGRKHPQQEYISINTRNILFICGGAFVGLDTIVRQREGRQTIGYGEP
                     KRKGRDAQSRDGHPVPLAVQPEDLIRFGLIPEFVGRLPVLTTMSEMTEDDLVRILTEP
                     RNCLVRQYQKLLDMGGVKLDFDEDALREIAALAMKRKTGARGLRAVMEEFMMEIMYDA
                     PGNPKLKAIRVTAEMIRSRFGNAA"
     assembly_gap    3018..3117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3198..4556)
                     /locus_tag="PLZ74_07455"
     CDS             complement(3198..4556)
                     /locus_tag="PLZ74_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR98195.1"
                     /translation="MFTRRNLLRLVLFVLCALAAAGLMPEAWRRGSVLLPGLSPLLSL
                     GGALAARAAGVLTLLGLPLLILPLFAGRFFCWRLCPMGFAAETVSRINRRNTGWIRKI
                     PFVGKGLALLILGSAAAGWPVLIWTDPLSVFNGFFAAARQPLTWLSAATASGFILILL
                     ASLIVPNLWCHRLCPLGGLQEWLSQLGKSLRSARCAKEGDEIPPLLTHARTGRRVFLG
                     FALGGVSGSLYRAYAPRRVTTDTDCGGTHSCGGRHGHGPGEGEAGQRRRMGRGSGAGL
                     PIRPPGAVQQAFNALCARCGNCMTACPYGLILPDLGTTGPDGLFTPVLAFRNLNPQQE
                     QFCFQECTACTQACPTGALRPLTVARKQQTAIGLAHICRNKCIAWKKGEYCVVCQEFC
                     PYQAIREVERNGVMCPIVDADKCRGCGACESQCPALPIAVTIRGRDPQTVLAHPSPAL
                     HT"
     gene            complement(4568..5473)
                     /locus_tag="PLZ74_07460"
     CDS             complement(4568..5473)
                     /locus_tag="PLZ74_07460"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOR98196.1"
                     /translation="MKPVKRRSFLKTGVTAAGTALVQTPLAAADAASPAATVIVVHGT
                     DIPKMLEAGIAKLGGWQAFIRTGMDVVIKPNVAWNSKPEQGGNTHPDLVRACVLAAEK
                     QGAATVSLPENTCHPEKATFQASGIAAALEGTRGRLYRPAKQDYGEAEIPKGKIVKRA
                     FVPKDILNCDCLINMPVAKHHSGATLTLSMKNWMGSVSNEDRRAWHRDGLHQCIADIN
                     TFLRAKLVVIDATRIMLTKGPQGPGELAHPYEIILSTDPVAADAYAVTLFKKTPFDVP
                     HVRIAHEMGIGCGDLAKIRVERVEA"
     gene            complement(5808..6785)
                     /locus_tag="PLZ74_07465"
     CDS             complement(5808..6785)
                     /locus_tag="PLZ74_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOR98197.1"
                     /translation="MSWQDWRWQLRHAVTGTSPLLRAYGVDPDTAERVHRRYPVFLTP
                     YALAQLTPGEPQDPLNRMALPSAAELFREPGMTPDPFGEQGRRVACCPGLKQRFPDRV
                     LVMAHDRCALACRHCTRKGLLGRASVMRTDAELAAAVVWVRAHPRVREVLLSGGDPLL
                     LPDTRVARLVRAFAALPQIDAVRIGTRVPVTLPMRITPNLARRLGAFRKVWVNTQFNH
                     VREITPEAEEACARLVDAGIPVSNQSVLLKGVNDSVQALFDLCAGLQRIRVRPYYVFL
                     CDPVAGLAPFRVPLRTARQIERALAVRLGGLALPRFVMDQPGSMRKIPI"
     gene            complement(6807..7652)
                     /locus_tag="PLZ74_07470"
     CDS             complement(6807..7652)
                     /locus_tag="PLZ74_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOR98198.1"
                     /translation="MKNRIVLTAMAVGLCCAGCFKHREAPPRPVTASSLEEAAQKRAE
                     IECLNLAGQEISALPRELAAMPKLSVLWLRGAKGLTEFGVLSDLKALKRLDLSALGSK
                     AVPPEVCGLKELEHLYLADNALTALPPAIGGLSKLSYLNLDRNPLSSLPNELGALSEL
                     RWLRLNQNALTSLPLSCANLKRLQRLYLRGNRFDHVPACLKDLPLLEDLCLSQNPIKT
                     LPDWLTALPKLRQLDLDGCPLGALPANLSGWKRLQVLSLVRCPLPDEERARISAALPD
                     THIAF"
     gene            complement(7729..8148)
                     /locus_tag="PLZ74_07475"
     CDS             complement(7729..8148)
                     /locus_tag="PLZ74_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98199.1"
                     /translation="MRLCLTFFSLALGCAMVVKADARVMITEHRDVNGKSTYWYITQE
                     RLAQTPEWKPDVSLPLPVEKAAGKATEWLKKRTTKFTEWDIVSISLGKIWDSEIKNKW
                     YYSVSVNGTVQVDGIKANSFFSVIVLMDGTVVEPTSE"
     gene            complement(8317..8451)
                     /locus_tag="PLZ74_07480"
     CDS             complement(8317..8451)
                     /locus_tag="PLZ74_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98200.1"
                     /translation="MKLHENQEFPLAFQKNPSSAIPCVRGEMIDPDPDTDTDPDLDET
                     "
BASE COUNT         1688 a         2528 c         2728 g         1588 t
ORIGIN      
        1 tgagattcag gaggaggttc gctcgcggct ctttcggtcc ttgtacaagc ctgcgctgga
       61 gcagaagaaa atcgcgtttg tggcgctgcg ggatgtgggg gacatgctgt tttctcctga
      121 gacgggtatc agcttttcca tgaccgtgga tgtgaaaccg gaattcgagt tgccgaggta
      181 taaaaaaatc ccggtcgttt ttgaagagcc cgtggtcacc gaagagcagg tggacggcca
      241 gatacagcag ttgcgtgaag ccttcgccca gttcaaggag gcgggtgcgg acgatgagat
      301 ccgcgatggc gacatggtgc gcatcgattt cgccggcacg gtggacgggc aatcgctcgc
      361 tgagctggac ccggccgcga aggcgctcgc cggcgagtcg ggacagtggg tgctggttca
      421 ggagggccgg tttctgccgg agattctgca ggcgctgcgg gggttgaaac agggcgcgga
      481 gaccacggcg caggtgacgt tcggtgccga tcacccgatc acggcactgc agggcaagac
      541 cgcgacgtat tcggtgacgg tccgcgagat ccgcgcgcgc tctctgcccg cggatgagga
      601 actgtgcgga cggttgaagg ttgcgtccat cgacgaaatg cgcacgcaga cccgcgaggg
      661 catgatggag gcggcgcgta agtccgagcg gatacgccgc gagcagacgg tcatcgactt
      721 tctgttgaaa caggcggatt tcgatttgcc ggaatccgag gaggcggagg agatcaacac
      781 catactcgac cagatgatgg gcgaggcgca gagtcgcggc ctgacacgcg aggatctgga
      841 gaagagccgc gggcagatca tcgagaacgc gacaacaacg gcgcggcggc agttgcggct
      901 gcgctacatt ctggcgcgca tcgcggagaa agaggcgctc acgctcagtc cggaggagat
      961 cgacagccga atcgccgaat tcgcgaacgc atcccgctcc acccccgcgc agttccgtgc
     1021 gcaggtggaa aaaaacggac ggatggatgc gctgcgcgaa cacatgctca acttgaaaac
     1081 catccagttc ctgcttgatc aggcgaaagg gtgaggagga cgaaacatgg agtcaccgca
     1141 agcgaatatg ctgatcccga tcgtggttga acagaccgga cggggcgagc gcgcatacga
     1201 catctactcg cggctgttgc aggaccgcat tattttcatc ggcaccgcca tcacggacga
     1261 ggtcagcaat gtgatcatcg cgcaattgct cttcctccag gcacaggatg ccaccaagga
     1321 gatttcggtg tacatcaact cccccggcgg ctcggtcacc gcgggtctgg cgatctacga
     1381 taccatgcag ttcatcagct gtgatgtcgc cacgtactgt gtgggccagg cggcgagtat
     1441 gggcgcggta ctgctggctg cgggcagcaa gggcaagcgc ttctccctgc ccaacgcgcg
     1501 catcatgatt caccagcctt ggggcggcgc ggagggcaag gccaccgaca tcagtatcgc
     1561 ggcgaaggag atcctgcgcc tgaaagaccg gctcaacgag attctcgccc atcataccgg
     1621 caagagcgtc gacgtgctgt cgaacgatac cgaacgcgac tatttcatgt ctgccgacga
     1681 ggccaagacg tacggactcg tggacgatgt gctgacccaa tctcttgcgg agaaatgatc
     1741 catggcgaag cggcccccga aaaaagcgat gctcacctgt tccttctgcg ggagggggga
     1801 tcgtgccgat gcggtggtca tccccggccc cgacgtgaac atctgcagcg actgcgtcga
     1861 catctgcacg cggatccttg cggaaaacaa cgcgaccccc gcatcgtctg ccgcgttgcc
     1921 cgtgctccac gtcccgccgc ccggcgagat caagacgttt ctcgatcagt acatcgtcgg
     1981 ccacgaccgc accaaaaaaa tactcgcggt cgcggttcac aaccactaca agcgcctcaa
     2041 tcagccgctc acgaaaaagg acgccttcgc ggatgtggag atcgagaaga gcaacatcct
     2101 gctgatcgga cccacggggt gcggcaagac gcttttcgcc cgctcgctcg cccggatgtt
     2161 ggatgtgccg ttcgcgatcg ctgacgccac gacccttacc gaggccggat acgtgggcga
     2221 ggatgtggag aacatcctgc tctacctgtt gcaggcggcg gacatgaatg tggccagggc
     2281 cgaacgcggc atcgtgtaca ttgatgagat cgacaaaatc gcgcgcaaga ccgagaacgt
     2341 ctccatcacg cgggacgtgt ccggcgaggg tgtgcaacag gcgctgctca agatcatcga
     2401 gggcacggtt tcgcgcgtac cgccgcaggg cggacgtaag catccgcagc aggagtacat
     2461 ctcgatcaac acgcgcaaca tcctctttat ctgcggcggt gcctttgtgg ggttggatac
     2521 gatcgtgcgg cagcgcgagg gacgccaaac gatcggatac ggggagccga agcgcaaagg
     2581 gcgcgacgct cagtcccgcg acgggcatcc ggtgccgctc gccgtgcaac cggaagacct
     2641 gatccgcttc gggctcattc ccgaattcgt gggccgtttg ccggtgctga ccaccatgag
     2701 cgagatgacc gaggacgatc tggtgcgcat cctgaccgaa ccgcggaact gtctggtgcg
     2761 gcagtatcag aaactgctcg acatgggcgg ggtgaagctc gacttcgatg aggacgcgct
     2821 gcgcgaaata gctgcgctgg ctatgaagcg taaaaccgga gcacgcggac tccgggccgt
     2881 gatggaagag ttcatgatgg agatcatgta cgatgcgccc ggcaatccga aactcaaggc
     2941 catccgcgtc acggcggaga tgattcgctc ccggttcgga aatgccgcgt agtcggtctc
     3001 cctcccgagg cgtccgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     3121 gaggcgtccg tatggtggat ccgcatctgc gaaatccgta tcatctgtgg ataaaaaacc
     3181 gatgcgtcgg cccttgctca cgtatgcagc gcgggtgagg gatgcgcgag caccgtctgc
     3241 gggtcgcgcc cgcggatcgt gacggcaatc ggcagcgcgg ggcactggct ctcgcacgcg
     3301 ccgcacccgc ggcatttgtc cgcatccacg atcgggcaca tgacgccgtt gcgctcgacc
     3361 tcacggatcg cctgataggg gcagaactcc tggcagacca cgcagtactc gcctttcttc
     3421 caggcgatgc acttgttccg gcagatgtgg gcgagtccga tagcggtctg ttgcttccgc
     3481 gcgaccgtca acgggcgcag tgcgccggtg gggcaggctt gtgtgcaggc ggtacactct
     3541 tggaagcaga actgctcctg ctgcggattg aggttgcgaa aagccagcac cggcgtgaac
     3601 agaccgtctg gaccggttgt tcccagatcc ggcagaatca ggccgtaggg acaggctgtc
     3661 atgcagttgc cgcaacgggc gcagagcgcg ttaaaggcct gctggaccgc acccggcggc
     3721 cggatcggga gacccgcccc tgagccgcgc cccatgcgcc gtctctgccc tgcctccccc
     3781 tctcccggac cgtggccgtg gcggccgccg caagaatgcg ttccgccgca gtccgtgtcg
     3841 gtggtaacac gacgcggagc gtacgcacgg tagagcgagc cggagacgcc tccgagcgcg
     3901 aatccgagaa aaacgcggcg tccggtccgg gcgtgcgtca gaagcggagg gatttcgtcc
     3961 ccctccttcg cgcaccgggc tgaacggagg cttttaccaa gttgcgacag ccactcctgc
     4021 aagcccccca gcgggcaaag ccgatggcac cagaggttcg gcacgatcag actggccagc
     4081 aggatcagga tgaacccgct ggcggtggcg gccgaaagcc aggtcaacgg ttgtcgcgct
     4141 gccgcgaaga atccgttgaa cacactcaac ggatcggtcc agatcagcac cggccatccc
     4201 gctgcggcgc tgccgaggat cagaagcgcc agacccttgc cgacaaacgg gatcttgcgg
     4261 atccaaccgg tgttgcggcg gttgatacgg ctgactgtct cggcggcgaa gcccatgggg
     4321 cagagtcgcc agcagaagaa gcgtcccgcg aaaagcggca ggatcagcag cggcagtccg
     4381 aggagcgtga gcacgcctgc ggcgcgggcc gcgagggcgc cgcccagact gagcaggggg
     4441 cttaggcccg gcagcagcac actcccgcgc cgccacgctt ccggcatgag tccggctgcc
     4501 gcaagggcgc acaaaacgaa caacacaaga cgaaggagat tacggcgcgt gaacatgtcg
     4561 tgtctcgcta cgcctccacc cgttccaccc ggattttggc gagatcgccg cacccgatcc
     4621 ccatctcatg cgcgatgcgg acatgcggca catcgaacgg cgtttttttg aacagcgtga
     4681 cggcgtaggc gtcggctgcc accggatcgg tgctcagaat gatctcgtac ggatgggcca
     4741 gttccccggg tccctgcggg cctttggtga gcataatgcg ggtagcgtcg atcaccacca
     4801 gctttgcgcg cagaaaggtg ttgatgtcgg cgatgcactg gtgcaggcca tcgcggtgcc
     4861 acgcgcgccg gtcctcgttc gagaccgagc ccatccagtt tttcatcgag agggtgagcg
     4921 tcgcaccgct gtggtgtttg gccaccggca tgttgatcag acagtcgcag ttcagaatgt
     4981 ccttggggac aaaggcgcgc ttcacgatct ttccctttgg gatctcggct tccccgtaat
     5041 cctgtttggc ggggcggtag agtcgacccc gtgtaccttc cagcgcggcg gcgataccac
     5101 tggcctgaaa tgtggctttc tcgggatgac aggtgttctc gggcagggat acggttgcgg
     5161 cgccctgttt ttcggcagcc agcacacagg cccggacaag gtcgggatgg gtgttgccgc
     5221 cctgttccgg cttcgagttc catgccacgt tgggtttgat caccacgtcc atgccggtcc
     5281 ggataaacgc ttgccagccg cccagtttcg cgatgcccgc ctcgagcatc ttcgggatat
     5341 ccgtgccatg gaccacaatc acggtggcgg caggagatgc ggcgtccgca gccgcaagcg
     5401 gtgtttgcac cagcgcggta ccggcggcgg tcactccggt tttcaagaaa gagcggcgtt
     5461 ttacgggttt catatgggtt ccctcttgca gtctcacagc cttacagcct tttgggttgc
     5521 gggcgccgcc cgcgttggtt tgtatggagc caagatacca catttgggaa atcaggcgcg
     5581 atcttttttc gtgaccgtac tgccgattta ggcatatccc cgtttctctg gccgttaagc
     5641 ctataaacgg caggtggacg tgcgaatttt gggtaaaggc ttgtaaacat tcgaaccatg
     5701 aaacatgaaa tcgggaatgg ggcaagcgtc tgcttgcccg gacgggcagg gagtcgggag
     5761 cgcgggcgcc ctcgcccgca actcggttcc gggtttcggg ggttcggtca gatcggtatt
     5821 ttacgcattg atccgggctg atccatcacg aaacggggca acgctaaacc gcccagacgc
     5881 acggccaggg cgcgttcaat ctgccgtgcg gtccgcaacg gcacgcggaa cggggcgagc
     5941 cccgcaaccg gatcgcagag aaaaacgtaa tacggccgca cgcggatgcg ctgcaggccc
     6001 gcgcagaggt cgaacaacgc ctgcacggaa tcgttgacgc ccttcagcag cacgctctgg
     6061 tttgataccg ggatgcccgc atccaccaga cgcgcgcagg cctcttcggc ttccggggtg
     6121 atctcgcgca catggttgaa ttgcgtgttg acccacacct tacggaacgc acccaagcgg
     6181 cgcgccagat tgggggtgat ccgcatcggc agggtcaccg gcacacgcgt gccgatccgc
     6241 accgcatcga tctgcggcag tgcggcgaag gcacgcacca gccgcgccac ccgcgtatcc
     6301 gggagcagca gcggatcacc gccggaaagc agcacctcac gcacgcgggg atgcgcccgt
     6361 acccagacta cggccgccgc aagttccgcg tcggtgcgca tgaccgacgc gcgtccgaga
     6421 agtcctttgc gcgtgcagtg gcggcaggcc agcgcgcatc ggtcatgcgc catcaccagt
     6481 acgcggtcgg ggaaacgctg tttgagaccg ggacagcacg ccacccgccg cccctgctcg
     6541 ccgaacgggt cgggggtcat gcccggctcg cggaacagtt ccgcggctga ggggagggcc
     6601 atacggttca gcggatcctg cggctcgccg ggggtgagtt gcgcgagcgc gtagggggtc
     6661 aaaaacaccg gatagcggcg gtgtacccgt tccgccgtat cgggatccac tccgtacgcg
     6721 cgcaacagcg gggaggttcc ggtgaccgca tgccgcagtt gccagcgcca gtcctgccaa
     6781 gacattgggc gttgagggct cacgggtcag aacgcgatgt gggtatccgg aagggccgcg
     6841 ctgatgcggg cccgctcctc atccggcagc ggacagcgca ccagcgagag gacttgcagg
     6901 cgtttccacc cgctgaggtt tgcgggcaac gcgccgagcg gacagccgtc gagatcgagt
     6961 tgacgcagtt tcggcagagc cgtgagccag tccggcaggg ttttgatcgg gttctgactg
     7021 aggcagagat cctcaagcag cggcagatcc ttcaggcaag ccggtacgtg gtcgaaccgg
     7081 tttccccgca ggtaaaggcg ttgcagccgt ttgaggttcg cgcaagacag cggcagggag
     7141 gtcagcgcgt tctggttgag acgcaaccag cgcaactcgg acagcgcccc cagttcattc
     7201 ggaagcgaag acagcggatt ccggtccaga ttgagatagc tcagtttgct cagcccgccg
     7261 attgccgggg gcagggcggt tagggcattg tccgcgagat agaggtgctc cagttccttc
     7321 agaccgcaca cctcaggcgg cacggctttg cttcccagag ccgagaggtc cagccgtttc
     7381 agggctttca gatccgacag aaccccgaat tcggtaagac ccttggcccc gcgcagccaa
     7441 agcacggaaa gtttcggcat cgcggccaac tcgcggggca gcgccgagat ttcttggccc
     7501 gcaaggttca ggcattcgat ctccgcccgt ttttgcgcgg cctcctccag actcgaggcg
     7561 gtgacggggc ggggcggtgc ctcacggtgc ttgaagcaac ccgcgcaaca caaccctacg
     7621 gccatcgctg tcagtacgat ccgattcttc atacgcgttt ccagtccttg ttaatgagaa
     7681 ccagcataca cgaaacgccc ggtggttggg gaggtttttt tggtggcctt attcgcttgt
     7741 tggctcgaca acggtaccat ccatcagaac gataacactg aagaacgaat ttgctttgat
     7801 gccatccacc tgaacggttc cattcacgga aacagagtaa taccatttat tctttatttc
     7861 cgagtcccat attttgccta gactaatgga gacaatatcc cattcggtga attttgtggt
     7921 gcgctttttt agccattctg ttgcttttcc tgccgctttc tcaactggca ggggcaaaga
     7981 aacgtcaggc ttccattcag gggtctgcgc aagccgttct tgtgtaatat accagtaagt
     8041 tgacttgccg ttcacgtcac gatgttcggt tatcatcaca cgcgcatcag ctttgacaac
     8101 catcgcacaa ccaagtgcca agctaaaaaa cgtcaaacag agtctcattg cccatcctcc
     8161 ttgttaccaa gatcaacccg cacattccca tcagcagcaa gttcaccgaa agggtacgct
     8221 tcttttgggg gcagcgcaag ggtttttggg gaacgccagg ggggattcct gattttcatg
     8281 aaggttcata tgtttcaggg tttctggttt ctggtttcag gtttcatcca aatcgggatc
     8341 ggtatcggta tcgggatcgg gatcgatcat ttcaccacga acacacggga tggcactgct
     8401 cgggtttttt tggaacgcca gggggaattc ctgattttca tgaagtttca tgtgtttcag
     8461 ggttccatcc aaaccgggat cggggtcggg ataacgcgtg tgcacatgcg cgcaggcctc
     8521 aatcgtgttt caatgggggc gcggcttgac aggttcgggg aggagttcgt aaactgaacc
     8581 gcacggatag ggtcgccatc gcggagtgcg ttcagtggca ctgcattgtt gg
//
LOCUS       DAOSRB010000120         8536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_7877_length_8536_cov_5.603231, whole genome shotgun sequence.
ACCESSION   DAOSRB010000120 DAOSRB010000000
VERSION     DAOSRB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7877_length_8536_cov_5.603231"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLZ74_07485"
     CDS             <1..563
                     /locus_tag="PLZ74_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOR98201.1"
                     /translation="LAERIPEIDAKKSVATGCSRLAKATLLATVRDERFAVCVPNQTG
                     GGGVPLAKRDFGENVSTEMNMFHHWYCKAYGKYIDNEQAMKFDQHLLVAAVAPRRILV
                     QGYNQGWFDTKGEFLACRAASAAWEFLGLPGLPAGDFPANYDTGRIGTHLGYVRRGGQ
                     HGISGYDWVWMLDFADVAFGKLRDAP"
     gene            1045..2169
                     /gene="prfB"
                     /locus_tag="PLZ74_07490"
     CDS             1045..2169
                     /gene="prfB"
                     /locus_tag="PLZ74_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOR98202.1"
                     /translation="MVMEFDEVKERLETMREKLDEMRGYLDIANRRALLAELEEAAAR
                     PDFWNDPNAAQEVIGKTNAQRAFVRPFDELVRQIEDGEVMLELAVAEKEGEQRQQALA
                     ELTNMFGKADVMFSKLRLQSLLGGKLDACNAYLTLHSGAGGTESCDWADMLFRMYRMY
                     CEDNGFEMEVLDSQPGDEAGLKSVTFLVSGPYAYGYLKAERGVHRLVRISPFDANKRR
                     HTSFCSLDVVAELTDEIEVEIKEEDLRVDTYRSSGAGGQHVNKTDSAIRLTHMPTGIV
                     VAVQSERSQHKNRSKAMSILKAKLYEYYEDKKRKEMERFYGEKGEIAWGSQIRSYVFQ
                     PYTMVKDHRTDEQTSNVQAVMDGRLQPFIEAYLRQNAVKV"
     gene            2267..3595
                     /locus_tag="PLZ74_07495"
     CDS             2267..3595
                     /locus_tag="PLZ74_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HOR98203.1"
                     /translation="MRMTFGQAKEVLARHGQMQVMRFWRSLDRAARGALLAQIASIDF
                     SEVARMRKLLAQGTRAVPVTPRPAPVVTWTRRMRAEALAKGQEQLRKGRVAALLVAGG
                     QGSRLGYEGPKGAYGIGPVSNLPLFYFHARKLVRLARLYGKPVPFYIMTSEANDAATR
                     ACFEDYNYFGLHPNDVVFFKQGLWPALDEAGHLILETPGRIFMSPDGHGGTLTALDRN
                     GCLQDMAARGIRNVFYFQVDNPLVEIADPAFVGLHVLRRADLSLKLCRKRSPTEKMGA
                     VSALDSGRFAMIEYSEMTPKQAARFRYGSPAIHIFSIAFLNREARKNLPLHLAHKKIA
                     CVDEAGRTVMPDAPNGYKFEKFIFDVLPDAKTVVNLAFDREEEFSPVKNAVGEDSPAT
                     CRMALQAKWRRWLAKAGVIVPESIPVEIDPAYAGGAEELKRGLLVQDIMI"
     gene            3697..3861
                     /locus_tag="PLZ74_07500"
                     /pseudo
     CDS             3697..3861
                     /locus_tag="PLZ74_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            3854..4075
                     /locus_tag="PLZ74_07505"
     CDS             3854..4075
                     /locus_tag="PLZ74_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98204.1"
                     /translation="MIDIETILNKACDILTEEARMTGFKSSPLFEQRVRNVLAALMPD
                     NFGIKVDMSPPAQAFPDGNHQKFCFWMNR"
     gene            4092..5651
                     /gene="gpmI"
                     /locus_tag="PLZ74_07510"
     CDS             4092..5651
                     /gene="gpmI"
                     /locus_tag="PLZ74_07510"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOR98205.1"
                     /translation="MRPVVLIVRDGWGLNPREDGNAVFAAQTPVIDRLKARYPWTRLA
                     CSGEAVGLPEGYQGSSEVGHLNMGAGRVVVQELKRIDDGLSTGELFKSAKWAALIANW
                     KEHKSALHFMGLLQDEGVHAHQEHLFKLMRRAREEYPEGRIVVHPFLDGRDTPPRSTL
                     EYLARLEKEMAAVGNCVIGTVQGRYYAMDRSKNYALTDTAYACLTAGEGRKAATAAEA
                     VQESYDKDQTPDNTPMVDEYIPPYVIGEYAGMKDGDSVMHFNYRQDRAIQLSQAFVCA
                     DYAGKRASSPRVRYLGFTRYWNEFSEYLLGAMGGDGGMDNLLGEVIAKAGLRQLRIAE
                     TQKFRHVTSFFNGKSTSPYPGEDQVDVPSRFDPATFASHPEMEAYNVTDELLKRLQDN
                     PYAFVAVNYANCDMVGHTGDFEATRRAVEVVDECIGRLLPRLFELDAHVLITADHGNA
                     EQMKDYETGANKTSHTLNEVECLYVAKDSAGVKLVEAGKLSDIAPTVLFLLDLPVPQE
                     MTAGNLIKSAG"
     gene            5936..7135
                     /locus_tag="PLZ74_07515"
     CDS             5936..7135
                     /locus_tag="PLZ74_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HOR98206.1"
                     /translation="MRIVFLIAVFVVQSAVAAIKFSNSYYSPRNRERPIRRSTSLIVL
                     HTTEAPSSSALRKLRDLGECHYCVDERGIVYRIIDHRREAYHAGRSMWNGRANVDSFS
                     VGIEMCGYHNKPLNAAQFRSLSALIGELQYIYKIPDHQVLTHAHVAYGAPNKWHRRSH
                     RGRKRCGMLFALPAVRARLNLKSRPKSDPDVRARRLVVGDAYLANVLYGSTSVGQASA
                     VPTLRAAEENVIAKGRSAWDIARDAYNDPTTLYRYPDGSVKRGNQIANFKQIPAGTRV
                     TVRADVRENRSETYQVIGVNGRAQDIAGEEARSASTVYVYPDGTYARGSQLSAASVLR
                     LPYGTRVLTGYAIGGPVTASRKATDICGNRWRSAETFFLISGALVPGNKVDDSRIPVG
                     TMLFFKN"
     gene            complement(7125..7676)
                     /locus_tag="PLZ74_07520"
     CDS             complement(7125..7676)
                     /locus_tag="PLZ74_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOR98207.1"
                     /translation="MATVLLGVTGSIAAYKACELTRLLVKAGNDVHVVMTQAAMRFVS
                     PLTFRTLSRNPVPTDSFAEPETWMPGHISLAERADILIIAPCTANVIAKLAYGLADDL
                     LTATALASRAPLVIAPAMNSGMWEHPATQANLGLLASRGVRLADAGTGDLACGTQGKG
                     RMAEPQVIFDLVSATLQSEALNS"
     gene            complement(7714..8307)
                     /gene="gmk"
                     /locus_tag="PLZ74_07525"
     CDS             complement(7714..8307)
                     /gene="gmk"
                     /locus_tag="PLZ74_07525"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOR98208.1"
                     /translation="MKPLFLVLSAPSGGGKTTLCDLLLQHYPEFCYSVSCTTREPRID
                     EEDGVDYYFLPMETFKRMIAEDRFLEYATVHDHYYGTLKEPVYDTLREGQCMLLDIDV
                     EGAHQIRTHVAGLPADNPLRQGYVDLFINPPSLEVLTERLQGRATDRQEVIEKRIRNA
                     QAEIARANEYMYQVTNDDLKTTFRRICDIINVRAGFI"
BASE COUNT         1762 a         2245 c         2802 g         1727 t
ORIGIN      
        1 accttgccga acgtattcca gagatcgatg cgaagaagtc cgtggccacc ggctgctcac
       61 gtctggcaaa ggctaccctt ctggccactg tccgggacga gcgtttcgcc gtatgcgttc
      121 ccaaccagac aggtggcggt ggtgtgcctc tcgccaagcg cgacttcggt gagaacgttt
      181 ccaccgagat gaacatgttc caccactggt actgcaaggc ttacggcaaa tacatcgaca
      241 acgagcaggc gatgaagttc gaccagcatc tccttgtggc ggcggttgcg ccgcgccgta
      301 tcctcgttca gggatataat caggggtggt tcgacacgaa gggtgagttc ctcgcttgcc
      361 gtgcggcatc tgccgcatgg gagttcttgg ggcttccggg tttgcctgca ggcgatttcc
      421 ctgccaacta cgacaccggc cgcatcggca cccatcttgg ttacgtgcgg cgcggcggtc
      481 agcacggcat ctcaggatac gattgggtat ggatgcttga cttcgccgat gtcgcgttcg
      541 gcaaactccg cgacgcgcca tgaaaccggg agtgccatca gcagacgcgg acgaatcgct
      601 cggtgatcgc gcggattttg ggcaaaagct cgtttgatcc gtacccgttc acagcgcagc
      661 aggcacactg cccgtgcacg aaaacgagaa cgtgtacggg gacgaggacg agtacgtgtg
      721 cggggaaacg attccgattc tgacagcgat gccttaggcc gggaggggag gggcaagcat
      781 ttgcttgccc ggacgagcag acgctcgtcc ccgtcatttg atttcatgat gttttgtggt
      841 ccggaatcgg gaacccggaa cccgaaacca agcacgatca cgggaaatcg ggtcgtttcc
      901 ctcttgcgga cttccgttgt ttaaccaccg tcaatacagc aggaacgcgc cctgaacgtt
      961 tcggtctgcg gacttgcact tgcggggttg gctcaatccc gatatagtaa cgctttcttt
     1021 ttctctacag tagtaagacg gacgatggtg atggaattcg atgaggtaaa agagcgtctg
     1081 gaaacgatgc gcgagaaact cgacgagatg cgcgggtatc tcgatatcgc gaaccggcgc
     1141 gccctgctcg cggaacttga ggaagcggct gcccgtcccg atttctggaa cgacccgaat
     1201 gcggctcagg aggtgatcgg caagaccaac gcacagcgtg cgtttgtgcg gccgtttgac
     1261 gagttggtcc gacagatcga agacggtgaa gtgatgctgg aactggcggt cgccgaaaag
     1321 gagggggaac agcggcaaca ggcgttggct gaactgacga acatgtttgg caaggcggac
     1381 gtaatgttct ccaaattgcg tttgcagtcg ctgctgggag gcaaactcga cgcctgcaac
     1441 gcctacctga cgctgcattc cggtgcgggc ggtacagagt catgcgattg ggctgacatg
     1501 ctgttccgca tgtatcggat gtattgcgag gacaacggtt ttgagatgga ggtgctcgac
     1561 tcccagccgg gggatgaggc agggttgaaa tcggtgacgt tcctggtttc cggaccgtat
     1621 gcgtatgggt atctcaaggc cgaacgtggt gttcaccggc tcgtgcggat cagccccttc
     1681 gatgccaaca agcgacggca cacgtcgttc tgttcgctgg atgtggtggc ggaactgact
     1741 gatgagattg aggtcgagat caaggaagag gacttgcgtg tggacaccta ccgctccagt
     1801 ggcgcggggg ggcagcatgt gaacaagacc gactccgcga tccgcctgac ccatatgccc
     1861 accgggattg tggtggcggt gcagtccgag cggtcgcagc acaagaaccg ctccaaagcc
     1921 atgagcatcc tcaaggccaa gctgtacgag tattatgagg ataagaaacg caaggagatg
     1981 gagcgttttt acggcgagaa gggggaaatc gcatggggca gccagatccg ttcctacgtg
     2041 ttccagccct atacgatggt aaaggatcac cgcacggatg agcagacctc taacgtgcag
     2101 gcggtgatgg acgggcggct tcagccgttc attgaagcct atctcaggca gaacgccgtg
     2161 aaggtgtgaa gaagggcccg ccccctccgg ggggggcggg aataaacgaa agaggaaccg
     2221 gaacaccgca cgtttagagt gtaacgttca ggttccgggg agacgcatgc gtatgacatt
     2281 cggacaggca aaagaggtgt tggcacggca cggccagatg caggtgatgc gcttttggcg
     2341 aagtttggac cgtgccgcgc gtggcgcgtt actggcacag atcgcgtcga tcgatttcag
     2401 tgaggttgcg cgcatgcgga aactgttggc gcagggaacc cgtgccgttc ccgtcacgcc
     2461 ccgtcccgca ccggtcgtga cttggacccg tcggatgcgt gccgaagccc ttgcgaaggg
     2521 gcaggagcag ttgcgtaagg gacgcgtggc cgctttgctg gtggcgggtg gccaaggatc
     2581 gcgtctgggg tatgagggcc cgaaaggcgc ctacgggatc ggtccggtgt cgaatttgcc
     2641 gctcttttac ttccatgccc gcaagctcgt tcggcttgcg cgcctgtacg gcaaacccgt
     2701 gcctttctat atcatgacca gcgaggcgaa cgacgccgca acacgcgcct gtttcgagga
     2761 ttacaactat ttcgggctcc accccaatga cgtggttttc tttaaacagg gtttgtggcc
     2821 agcgctggat gaggcgggcc atctgattct ggagacgccc ggacgaatct tcatgagtcc
     2881 ggacggtcac ggcggcacac tcaccgcgtt ggaccgcaac ggttgtctgc aagacatggc
     2941 cgcgcgcggg atccgcaatg ttttctattt tcaggtggat aatccccttg tggagatcgc
     3001 cgacccggcg tttgtgggtt tacacgttct ccgccgcgcc gatctctcgc tcaaactctg
     3061 ccgcaaacgt tctcctacag aaaagatggg ggcggtctct gcgcttgatt ccggacgctt
     3121 cgccatgatc gaatattccg aaatgacgcc gaaacaggcc gcgcggttcc gttacggcag
     3181 tcccgcgatc cacatctttt cgatcgcatt cctcaatcgg gaggcacgga agaacctgcc
     3241 gttgcatctg gcccataaga agatcgcctg tgtggatgag gcgggacgca cggtcatgcc
     3301 ggacgccccg aacggttaca agttcgagaa attcattttt gatgtgttgc cggacgcgaa
     3361 gacagtggtg aatctggcat ttgaccggga ggaggagttc tcgccggtca agaatgcggt
     3421 tggcgaggat tcgcctgcca catgcaggat ggcccttcag gcgaagtggc ggcggtggct
     3481 cgcgaaggcg ggtgtgatcg tgccggagtc gatcccggtc gagatcgatc cagcgtatgc
     3541 gggaggcgcg gaggaactta agaggggctt gttggtgcaa gatattatga tataattgat
     3601 ttgaagggag tcggtgtgac gacatgggtg atacctagaa tggagaagca gcggagaatc
     3661 aagagttctc tccggcacat ggctttggcg gacaggcgtg tggcggcacg aatacaatct
     3721 ttccctgaca gcttccgctt tttttgtggt ctccgggaaa ccgaacgaca gatagggaat
     3781 gcggtaccgc ccgttctggc ttggcacgtc gcccgcgctg tcgcggcgat attgcgggag
     3841 ggctcatctt cacatgattg acattgaaac catattgaac aaagcgtgcg acattttaac
     3901 tgaagaagcg cgcatgactg gtttcaaatc aagtccttta tttgaacaaa gggttcgtaa
     3961 tgtgcttgcg gctttaatgc cggacaattt cggaataaaa gtcgacatgt ctccgcctgc
     4021 gcaggcgttt ccggatggaa accatcagaa gttttgtttt tggatgaaca gatgaaggag
     4081 agttgcaaac gatgagacca gtagttttga ttgttcgtga cggatggggg ttgaatccgc
     4141 gggaagacgg gaatgcggtg ttcgcggcgc agacgccggt gatcgaccgt ttgaaggcgc
     4201 ggtacccctg gacgcggctg gcgtgcagcg gcgaggcggt gggtctgccg gaaggttatc
     4261 agggttcaag cgaggtcggg cacctgaata tgggcgccgg gcgcgtggtg gtgcaggaac
     4321 tgaagcggat tgatgacggg ctgagcacgg gcgagctgtt caagagcgca aagtgggcgg
     4381 cactgatcgc aaactggaaa gagcacaaaa gcgcgttgca ctttatgggg ctgctgcagg
     4441 acgagggtgt gcacgcgcac caagagcacc ttttcaagct gatgcggcgg gcgcgggaag
     4501 agtatcccga aggccgcata gtggtgcacc cgttcttgga tgggcgcgac acgccgccgc
     4561 gcagcacgct cgagtatctg gcgcggttgg agaaagagat ggcggcagtg ggcaactgcg
     4621 tgatcggcac ggtgcaggga cggtattatg ccatggaccg ctcgaagaat tacgcgctga
     4681 ccgacacggc ttacgcctgc ttgacggcgg gcgaggggcg caaggcggct acggcggcag
     4741 aagcggtgca ggagtcgtat gataaagacc agacaccgga taacaccccg atggtggacg
     4801 agtacatccc gccgtatgtg atcggcgaat atgcggggat gaaggacggg gattcggtga
     4861 tgcacttcaa ctaccggcag gaccgtgcga tccagttgtc gcaggctttc gtctgcgcag
     4921 attacgcggg taagcgcgcg tcgtctccca gagtgcggta tctggggttc acccgttact
     4981 ggaacgagtt cagcgaatac ctgctggggg cgatgggcgg tgacggcggc atggacaacc
     5041 tgctgggcga ggtgatcgca aaggccggtc tgcggcagtt gcgtatcgcc gagacgcaga
     5101 agttccggca cgtgaccagc tttttcaacg gcaaatccac aagtccgtat cccggcgagg
     5161 atcaggtgga cgtgccgagc cgctttgacc cggcgacttt cgcgagccac cccgagatgg
     5221 aggcgtacaa cgtgacggac gagctgctca agcggttgca ggacaacccg tatgcgttcg
     5281 tggcggtgaa ctacgccaac tgcgacatgg tggggcacac cggtgacttt gaggcgacgc
     5341 gcagggcggt tgaggtcgtc gacgagtgta tcgggcggct gctgccgcgt ctgttcgagc
     5401 ttgacgcgca tgtgctgatc acggctgacc acggcaatgc cgagcagatg aaggattacg
     5461 agaccggcgc caacaagacc agccacacgc tcaatgaggt tgagtgcttg tatgtggcca
     5521 aggactcggc gggggtcaag ctggtggagg ctggcaagct gagcgacatc gcgccgacgg
     5581 ttctgttctt actggatctg ccggtgccgc aggagatgac ggcaggcaac ctgatcaaat
     5641 cggcagggtg agaggctgta ggtaagaggt aaatggtgag tggtgagtgg taggagcgcg
     5701 cgtaaacgcg ctacgcagga cggcaggatt ggtagcagcg gcgccgggat aggggaaggc
     5761 gggagcgcgg gcgaacggga cggctcgccg agccgtccgc ggcggtttcg gcgaaaccgc
     5821 cctaccggtt gtaacacggc tttggtatga tagaatggcc tttgcgtgag cttgtggatt
     5881 gggggatggc ttgtttttat gggataaatg gaaaaggagg gatgacgggg gcgtcatgcg
     5941 gattgttttt ctgatagcgg tgtttgttgt ccagagcgct gtggctgcga tcaaattcag
     6001 taacagttat tacagtccgc gcaaccggga gcggccaatc cgcaggtcaa cgtcgctgat
     6061 agtcctgcac acgacggaag cgccttcaag tagcgctttg cgtaaattgc gcgacttggg
     6121 ggagtgccac tactgcgttg acgaacgcgg gattgtctac cgcatcatcg atcaccggcg
     6181 cgaggcatac catgcggggc gcagcatgtg gaacggacgc gccaacgtgg attcgttttc
     6241 ggtcggtatc gagatgtgcg ggtaccacaa caagccgttg aacgcagcgc aattccgctc
     6301 gctgtcggca ctgatcggcg agttgcagta catatacaag atcccggatc atcaggtgct
     6361 cacgcatgcg catgtggcgt acggggcacc aaacaaatgg catcgacgga gtcatcgagg
     6421 gcggaaacgg tgtggcatgc tcttcgcgct gcctgcggtt cgtgcgcggc ttaatctcaa
     6481 gtcgcgtccg aagagcgatc cggatgttcg tgcgcggcgg ctggtggtgg gggatgccta
     6541 tctcgcgaat gttctgtacg gttcgacgtc cgtggggcag gcaagtgccg tgccgaccct
     6601 ccgcgcggcg gaagagaatg tgatcgccaa ggggcgtagc gcttgggaca tcgcgcgcga
     6661 tgcgtacaac gatcccacca cgctataccg gtatcccgac ggatcggtca aacgcgggaa
     6721 tcaaatcgcc aatttcaaac agatccccgc aggcacgcga gtcaccgtgc gggcggatgt
     6781 gcgggaaaac cggtcggaga cgtatcaggt gatcggggtg aacggtcggg cgcaggatat
     6841 cgcgggcgag gaggcccgga gcgcgtcaac ggtgtatgtc tatccggacg gcacctatgc
     6901 gcgcggcagt caattaagcg ctgcgtcggt tctgaggctg ccctacggga ctcgtgtgct
     6961 gaccggctac gcgattggcg ggccggttac ggcgagccgc aaagcaacgg acatttgcgg
     7021 taaccgctgg cgctccgccg agacgttttt tctcatctcc ggcgcgttgg ttcccggaaa
     7081 caaagtggat gacagccgga ttcccgtcgg cacgatgctg ttcttcaaga attgagcgct
     7141 tcggattgaa gtgtcgccga aacaagatcg aagatcactt ggggttcggc catacggccc
     7201 ttgccctgcg tgccgcaggc aaggtcgccg gtcccggcat ccgccaggcg cacgccgcgc
     7261 gatgcgagga ggcccagatt ggcttgtgtc gccggatgtt cccacatgcc ggaattcatg
     7321 gcgggcgcga taaccagcgg cgcgcgtgat gcaagcgcgg tggcggtcaa caggtcgtcc
     7381 gccagcccgt aagcgagttt ggcgatcaca ttggcggtac agggcgcgat gatcaggatg
     7441 tcggcgcgct ccgcgagcga aatgtgtccg ggcatccacg tttccggctc ggcgaacgaa
     7501 tccgtcggaa ccggattccg ggacagcgtg cggaacgtca gcggcgaaac gaatcgcatg
     7561 gcggcttgtg tcatcaccac atgcacatcg tttccggcct tcaccagcag ccgggtcagt
     7621 tcacaagcct tgtaggcggc gatcgaaccg gtcactccaa gcagtacggt tgccatactc
     7681 ctctcctttc ccgaaccttt ggcgcgaagc gcctcagatg aatcccgccc ggacattgat
     7741 gatgtcacaa atgcgccgga acgtcgtttt gagatcgtcg ttggtcacct gatacatgta
     7801 ctcgttggcg cgcgctatct cggcttgcgc gttgcggatc cgtttctcaa tcacctcctg
     7861 gcggtccgtg gcacggcctt gcagccgttc ggtcagcacc tccagcgagg gcgggttgat
     7921 gaagagatcc acgtaccctt gccgcaatgg gttatctgca ggcaggccag ccacatgcgt
     7981 gcggatctgg tgtgcgcctt caacatcgat gtcgagcaac atgcactggc cttcgcgaag
     8041 cgtgtcatac accggttctt tcagtgtgcc gtaatagtgg tcatgcacgg tggcgtactc
     8101 aaggaaccgg tcttccgcga tcatccgttt gaaggtttcc atcgggagga aatagtagtc
     8161 aaccccgtct tcctcatcaa tgcggggttc gcgggtggta caggaaacgg aataacaaaa
     8221 ctctggatag tgttgcagca acaggtcaca taaggtggtt ttacccccgc ctgacggtgc
     8281 ggacaacaca aggaatagcg gtttcatcac gattctcctt cacaaaacgc acacagtgta
     8341 cccgcttaca gggccggatc aaagtgcaaa tattggatgg gacctcggtt tccggttccg
     8401 taccatgaaa cacaagaaac agcagtcctg cggtttcccc ttgcctttcc gccatcgaat
     8461 ccgttacagt accaccgttt ccccgctcgg gtggtggaat ggcagacacg catgtttgag
     8521 gggcatgtgc cgcgag
//
LOCUS       DAOSRB010000121         8392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8028_length_8392_cov_6.972652, whole genome shotgun sequence.
ACCESSION   DAOSRB010000121 DAOSRB010000000
VERSION     DAOSRB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8028_length_8392_cov_6.972652"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..3117)
                     /locus_tag="PLZ74_07530"
     CDS             complement(124..3117)
                     /locus_tag="PLZ74_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOR98209.1"
                     /translation="MLILAALLPCGGILHAKNAVTEAYALKLWTVEDRLPGSPVVGVS
                     QASDGYLWLVTGAQLIRFNGVHFTEIAVPEEVRRQTGGFGGVFCGRAKGVWVYGPSGV
                     ACCRAGQWQVWSGAAVSGTAGRILSMVETPDGVVRAVAERGLLEAVAAADDLATQFVA
                     QLCPVPPDDRATLGAVTDADADGSGSIWMTAWNGLVEYRAGRYDDQSMRLPDFLVEAA
                     DGIHAGRSGRLWIHGSNGVAYREKSIWTPIGFPENAGMATVMLEASDGSLWIGNPTGM
                     FRWDAGVWRRLAEQDTPGSLSVNTLIEDRDGTLWAACDGGLLRIRKKSVEPLRAEGTA
                     AMGTAYGLWRTPEGGAWVGFKGRAARLTAREWRLLQTVYLDADVPVSALLQDSAGRVW
                     LGTLGGGLFLYQQGSLTLVPQRDYSMPAAHTVYTLMEDAEHGILAGTPQGLMRVNVAG
                     ELEQAVIRGALFAEPVYALYRDRDGALWICSEGTGLVCLRRDGAREGIGHEAGLEGYP
                     RVTYRDTRGQFWVGTTAGLFLLTPQALIPLGRQTGLFGDAVLQLSEDEAGRLWLGTTR
                     GLLGVVIEDLARLAQAGGKDETVAVRLLHLGVSDGMPGERCMGGVLSRGGAETGRLWF
                     PCENGVAVVDPQKVGFSGQPPTVVIERVLVNGQEVLNNVAGEPRTAVFAPGARNVVFH
                     FAALSPGAADSARYRYRVDGLRMAWSPVQAEQQAVFEWLPPGRYTLKVMAEAGGIWNL
                     NGAALAFEVRAYFWQTTWFYLVLALALAATVFLVARWILWHRYQLQMAILKREEALAQ
                     ERARISRDIHDDLGNGLSVVATLSELAHNDVEKGLVHKRLDQIYEVANELARNVDETV
                     WAVNPANDGWEPFLSYFEQYTEYFLGNSGLRFHFTRPAELVERPIASKTRHHLLLAVR
                     EAVGNVLKHASAKQVRIAMRLDGVVLEVRVEDDGVGFDPSACAGVGHDGLGNMARRMR
                     EAGGVMTLDSAPGAGTRVLFRVPLE"
     gene            complement(3153..4178)
                     /locus_tag="PLZ74_07535"
     CDS             complement(3153..4178)
                     /locus_tag="PLZ74_07535"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOR98210.1"
                     /translation="MKTPLPDTLPSDTFCLAPLRGVTVRAFRALHARWFTPPDYAVAP
                     FIPTFAGGKVKRTLLKDIDPALGQAVPVVPQVIGKEPLLLRTLLQVFKSMGYDGADLN
                     AGCPYPFIVKKGRGAGLMRDAGAFARMLEAGCEEMPQGFSVKVRLGIDRPDLLMERME
                     VINAFPLREVTIHARTARQMYAGRVDLAAFEEARRACRHPVVYNGDLCTRADFLLLKT
                     RFPMITRWMIGRGAAVDPFVFGRLTAEASPPRDAERLKRFLDEYLDACLEELHGPASV
                     LGRLKELWGYLHRSLTGGGRVWKRVRVCRTVDEYRRVVEESFGRAFPGFVEETERNGF
                     GPADRGD"
     gene            complement(4373..>4729)
                     /locus_tag="PLZ74_07540"
     CDS             complement(4373..>4729)
                     /locus_tag="PLZ74_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98211.1"
                     /translation="LEVAGTDAADLTAPNAAGWTHYAVTVNPSGSVQAYGNGEPLTTV
                     PNALAALLPVNQALTLGHAGDETDNTKTFSGVLDEVRIETVPRGADWIRAAYMTQAEN
                     ESFTSYNRWGTLMLMR"
     assembly_gap    4730..4829
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4830..>8392)
                     /locus_tag="PLZ74_07545"
     CDS             complement(<4830..>8392)
                     /locus_tag="PLZ74_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98212.1"
                     /translation="GAYSVFVVAQVVDASERGFTWRRVVPARDANWLLGTWPDGGTFY
                     AHAQHAMTDLSSCLKSVFTFNRVYTLAAVNTLTEQRFFVNGYDLTSDATRTTGPGRLT
                     IGGAGSISQDPSDALISEVIVYESALSATDRQQVEAYLAARYGVTPEGFDGPVWSGAG
                     SDHFWSTADNWQQALPAEPALAFVGSARRASMNDLTGLTLHHVLVRDNTWSFLGNPVS
                     LKTDFMSFTSGTVDWALDTVLPEGPHLFSVRNDGKLNMNGLLSGAGGVTAGMGTSFQG
                     TLNLACPSNTFTGPALLKYGITEVTRLANAGVPSSLGAAIGADAAIRLGNYYTGGYYG
                     MRYTGTEPFSTDRPFLFLRGVTIENNSPADAGGAFNGNWSAMEAPGGITATLTLAGTS
                     KGVNTVNGVIANAPGGANSLIVKITSGVWAFSAANTFTGGLTVEGGSVLVNGTDAGYL
                     GAGNVTVKAGAVLGGSGRLAGYSSLALLPGSVLVPGNPDVDGGIGTLTFSAAPALNGM
                     ELRCQATAQTNDLIRIETAWSPPGFMTVRVEADSEEACPERLTIIEAASLTGSADLSG
                     WDIEAPCAYRAVVEGNAVVLMKEPPPDLAAWQRSMPVRFSGYDGVSELTNFPALIKLS
                     DGCGNGRFRYADCLAGGADLLFTDETGARLDHEIETWNTNGESHVWVRVPRLVRNTEI
                     TAHWKNPARATDANPFLPTDLTGCAMWLHAGAGVTADGAGLVSRWADQSGNGHEAVRG
                     DSRYQPCLTNNVVNGLPGLRFEGDTYKDGLITTWGTSNGTYSVFVTASYQVSTTYSWY
                     RLATGPANYNWGIELNGSGGDFYSFIPNGAGGNEYYQIVNTGIRPAGGVPFTAAILGD
                     GTNTQFTLNGFGFTPIAKHHGPGTLILGHGGISGNPWLGHIFEVIVYSRALGFEERRQ
                     VERYLALKYGIRSAHLTPSAGLVQWLRAENAIADVADGAANRVKRWDNQTQYGSGVQA
                     TQATTNRMPEKIAGALNGKPVLRFDAAAGEYDSLKAVLASVDKTYSVIAVFAPRSADP
                     AERIVLQGVNGASRLSVTNGLLTRQASGTVSQLAPAQVNRAAIAVMTCDAQASRFYLN
                     GVNLTQNSAPVGLWKGTGSSVIYLGAGTGDGALDLPLDGDLAEVLVYDRLISDTERRR
                     IEADLSERYAIPVARVNPAVWSSEFGGVWHFTGTDRLLLADA"
BASE COUNT         1473 a         3003 c         2467 g         1349 t
ORIGIN      
        1 gaaaccctga aaccagaaac cgaagagcgt tgaaagttca actgctcctt ttagtgtgaa
       61 gggtcaacgt ggcgctgacc ttctgcccat acccttccac ccctggcttt ggcacgaatc
      121 ccctcattcc aacggcacac ggaacagcac gcgcgtaccc gcgcccggcg cgctatccaa
      181 ggtcatgacc cctcccgcct cgcgcatgcg ccgcgccatg ttccccaacc cgtcgtgccc
      241 cactcccgcg cacgccgacg ggtcaaagcc gaccccgtcg tcctccacac gcacttccag
      301 cacgacgccg tccagacgca tcgcgatccg cacttgtttt gcggaggcgt gtttgagcac
      361 attcccgacc gcttcgcgca cggcgagcag cagatggtgc cgggttttgg aggcgatcgg
      421 acgctccacc agttccgcag gccgcgtgaa gtggaaccgc agcccggagt tccccagaaa
      481 atactcggtg tactgctcga aataactgag gaacggctcc catccgtcat tggcgggatt
      541 caccgcccaa accgtctcat ccacattgcg ggccagctcg ttagccacct cataaatctg
      601 atccagccgt ttgtggacca atcctttctc gacatcgttg tgcgccagct cgctaagcgt
      661 ggccaccact gaaagcccgt tgccgagatc gtcgtggatg tcgcgcgaaa tgcgcgcgcg
      721 ctcctgcgcc agcgcctcct cacgtttgag aatcgccatc tgcaactggt accggtgcca
      781 caggatccag cgcgcgacca gaaagaccgt tgcagccaac gccagcgcca gaaccagata
      841 aaaccacgtg gtctgccaga aatacgcccg gacctcaaac gccagcgccg ccccattcag
      901 attccaaatg ccgcccgcct cggccataac cttcagggtg taacgtccgg gcggcaacca
      961 ctcgaacacc gcctgttgct cagcctgcac cggcgaccac gccatgcgga gtccgtcaac
     1021 ccggtagcgg taacgcgcag agtctgccgc tcccggcgag agggccgcga aatgaaacac
     1081 cacattgcgc gcgccgggcg cgaaaaccgc tgtccgcggt tcgcccgcca cattgttcag
     1141 aacctcctga ccgttcacca acacccgctc gatcaccact gtgggcggct gcccggaaaa
     1201 cccgactttc tgcggatcca ccaccgcaac cccgttctca caaggaaacc agaggcgacc
     1261 ggtttccgcg ccgccccgcg acaacacccc gcccatacag cgctcgccgg gcatcccgtc
     1321 cgaaacgccc aagtgcagta accggaccgc cacggtctcg tcctttccgc cggcctgcgc
     1381 caaccgggcc agatcctcga tcaccacgcc gagcagaccg cgcgtggtgc ccagccagag
     1441 ccgaccggcc tcgtcttccg agagttgcaa caccgcgtcg ccgaacagac cggtttgacg
     1501 ccccaacggg atcagcgcct gcggggtgag cagaaaaagc cctgccgtgg tccccaccca
     1561 gaactgcccc cgcgtgtcgc ggtaggtcac ccgcggatac ccctccagcc cggcctcgtg
     1621 tccgatcccc tcgcgggcgc cgtcgcggcg gaggcaaacc agtcccgtgc cctcgctgca
     1681 aatccacaac gcgccgtccc gatcgcggta cagggcatac accggttccg cgaacagcgc
     1741 gccgcggatc acggcctgct ccaactcccc ggctacattg acccgcatca gcccctgcgg
     1801 cgtaccggcc agaataccgt gttccgcatc ctccatcagc gtgtacaccg tatgtgccgc
     1861 cggcatgctg taatcgcgct gcggcaccag cgtgagactg ccctgctgat acaggaagag
     1921 cccaccgccc agcgtgccga gccagacgcg gcccgcgctg tcttgcagca aggcgctcac
     1981 cggcacgtcc gcatccagat aaacggtctg gagcaaacgc cactcacgcg ccgtcagcct
     2041 cgcggcgcgc cctttgaacc cgacccaggc gcccccctca ggtgtgcgcc acaacccgta
     2101 ggccgtgccc attgcagccg taccctccgc ccgcaacggc tccacgctct tcttccggat
     2161 gcgcagcaat ccgccgtcgc aggcggccca caaggtgccg tcccgatcct caatcagcgt
     2221 gttgacagag aggcttccgg gcgtgtcctg ttcagccagc cgccgccaaa ctccggcatc
     2281 ccagcggaac atgcccgtcg ggttgccgat ccagagcgaa ccgtccgacg cctccagcat
     2341 gaccgtggcc atccccgcgt tttcggggaa cccgatcggg gtccagatgc tcttctcgcg
     2401 gtacgcgacc ccgttcgacc cgtgtatcca caaacgcccc gaacgccccg cgtgaatccc
     2461 gtcggccgcc tccaccagaa aatccggcag ccgcatgctt tgatcatcgt accggcccgc
     2521 acggtactcc accagtccgt tccacgcggt catccagatg ctgcccgaac cgtccgcatc
     2581 cgcatccgtc accgcgccca gggtcgcgcg gtcatcgggc ggcaccggac acagttgcgc
     2641 cacgaattgc gtggcgagat catccgccgc ggccaccgct tcaagcagac cccgttccgc
     2701 aaccgcgcgc accaccccgt ccggcgtctc aaccatgctc aggatacgtc ccgccgtacc
     2761 ggaaaccgcc gcgccggacc acacctgcca ctggcccgca cggcagcagg ccaccccgga
     2821 gggaccgtac acccaaactc ccttggcgcg gccgcaaaaa accccgccga acccgccggt
     2881 ctgccgccgc acctcctccg gaaccgctat ctccgtaaaa tgcacaccgt tgaaccggat
     2941 cagttgcgca ccggtcacca gccagagata cccgtccgac gcctgcgaca caccgaccac
     3001 cggtgaaccc ggcaaccggt cctccacggt ccagagtttc agcgcgtacg cttcggtcac
     3061 cgcgttcttg gcgtggagga tcccgccgca cggcagcagc gccgcaagga tcagcatcca
     3121 tagaattctg aagtgaccgc gcatatccgc cttcaatctc ccctgtccgc cgggccgaac
     3181 ccgtttcgtt ccgtctcctc gacaaacccc ggaaaggccc ggccgaaaga ctcctccacc
     3241 acccgtcgat actcatccac cgtgcggcag acccgcaccc gtttccagac gcgcccgcct
     3301 ccggtgagtg accggtgcag atatccccac aactctttca gacgcccgag caccgatgcc
     3361 ggtccgtgca gctcctcgag acacgcgtcc agatactcgt cgagaaacct cttgagccgc
     3421 tccgcatcgc ggggcggcga cgcctctgcg gtgaggcggc cgaaaacaaa cggatcgacc
     3481 gcggccccgc ggccgatcat ccagcgcgtg atcatcggga accgcgtctt gagcaaaaga
     3541 aaatcggcgc gcgtgcacag atcgccatta tacacgaccg gatgccggca cgcccgccgc
     3601 gcctcctcga acgccgccaa gtccacgcgg cccgcataca tctgccgcgc ggtgcgcgca
     3661 tgaatcgtca cctcgcgcaa gggaaaggcg ttgatcacct ccatcctctc catcagcaga
     3721 tccggccggt cgatgcccaa ccggactttt accgaaaacc cctgcggcat ctcctcgcag
     3781 cccgcctcca gcatacgcgc gaaggccccg gcatcccgca tcagtcccgc gccgcggcct
     3841 tttttcacga tgaacggata cgggcagccc gcattgaggt ccgcaccgtc atagcccatg
     3901 gacttgaaca cctgcaacag cgtacgcaac aagagcggct ccttgccgat cacctgcggc
     3961 accaccggca ccgcctgtcc cagggcggga tcgatatcct taagcagcgt ccgtttgacc
     4021 ttcccgcctg caaaggttgg gatgaacggc gcgaccgcgt agtccggcgg ggtgaaccag
     4081 cgcgcgtgca acgcacggaa ggcccgcacc gtgacgccgc gcagcggcgc aaggcagaac
     4141 gtatcgctag gaagcgtgtc tggcaacggc gttttcatgt gcgtcgacct acgacccgcg
     4201 acattcgcct tgcggcgtct gggttgtccg gctgatccgc cattgcgggc gggaacgctc
     4261 cctttccagt gcggaccgca tgctggcctt ctactttctg accatgggga accgggcttt
     4321 tgccgaacgt ttgaaggccc gaggatactc ggcgcaaggc atcatggcgt gttcagcgca
     4381 tcagcatcaa ggttccccag cggttgtagc tggtaaagct ctcgttttcc gcctgggtca
     4441 tgtaggcggc acggatccag tccgcaccgc gcggcacggt ctcaatgcgc acctcatcca
     4501 acacgccgga gaaggtcttc gtgttgtccg tctcgtcgcc cgcatgaccc aaggtgagcg
     4561 cctgattgac gggcagaagt gcggccagcg cgttgggcac cgtggtcagc ggctctccgt
     4621 tgccataggc ctgcacgctc cccgagggat tgaccgtcac ggcgtaatgg gtccagcccg
     4681 ccgcatttgg tgccgtcaga tcggccgcgt ccgtacccgc gacctccagn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgtcggccag cagcaggcgg tccgtgccgg
     4861 tgaagtgcca cacgccgccg aattcggatg accacaccgc ggggttcacc cgcgccacgg
     4921 ggatcgcgta gcgttcggag aggtcagcct cgatgcggcg gcgctcggtg tccgagatca
     4981 gccggtcata caccagcacc tcggcgaggt cgccgtccag cggcagatcg agcgcgccgt
     5041 cgccggtccc cgcgccgaga tagatgacgc tgctgcccgt gcctttccaa agtccgaccg
     5101 gcgcgctgtt ctgggtcagg ttgacgccgt tgaggtagaa acgcgaggcc tgcgcgtcac
     5161 aggtcatgac cgcgatggcg gcgcggttga cctgcgcggg cgcgagctgc gagaccgtgc
     5221 ccgacgcctg ccgggtcaac agcccgttgg tgacgctcag gcggctggcc ccgttcacgc
     5281 cctgcagcac gatccgctcg gcggggtcag cggaacgcgg ggcgaaaacc gcgatcacgg
     5341 agtaggtctt gtccaccgac gccaggacgg ccttcagact gtcgtactcg cccgctgcgg
     5401 cgtcgaaccg cagcaccggc ttgccgttga gcgccccggc gatcttctcg ggcatgcggt
     5461 tggtcgtggc ctgcgtggcc tgcacgccgc tcccgtactg cgtctggttg tcccatcgct
     5521 tgacacggtt tgccgcgccg tcggcgacgt cggcgatcgc gttttcggcg cgcagccact
     5581 ggaccagccc cgcggagggc gtgaggtggg cggagcgtat cccgtacttt agcgcgaggt
     5641 agcgctccac ctgccgccgc tcctcgaacc ccagcgcgcg gctgtagacg atgacctcaa
     5701 aaatatggcc cagccacggg ttgccgctga tgccgccatg tcccagaatc agcgtgccgg
     5761 gtccgtggtg cttggcgatc ggcgtgaacc cgaagccgtt cagcgtgaac tgcgtgttcg
     5821 tgccgtcgcc caaaatcgcc gccgtgaacg gcacgccgcc cgcgggccgg atacccgtgt
     5881 tcacgatctg ataatactcg tttccgcccg caccgttggg gatgaaggag tagaaatccc
     5941 cccccgaccc gttcagctcg atcccccagt tgtaattggc cgggccggtc gccagccggt
     6001 accaactata ggtcgtgctc acctgataac tggcggttac gaacaccgaa taggtgccgt
     6061 tgctggtgcc ccaggtcgtg atcaacccgt ctttgtaggt gtccccctcg aagcgcaggc
     6121 cgggcagccc gttgaccacg ttgttggtca ggcagggctg gtaccgggaa tcgccccgca
     6181 cggcctcgtg cccgttgccc gactggtcgg cccagcgcga gaccagcccg gcgccgtcgg
     6241 ccgtcacgcc cgcgcccgcg tgcagccaca tggcgcagcc cgtcaggtcg gtgggcagga
     6301 aaggattcgc gtccgtcgcg cgggccgggt tcttccagtg cgcggtgatc tcggtgttgc
     6361 ggaccagccg cggcacccgt acccagacgt gcgattcgcc gttggtgttc caggtctcga
     6421 tctcgtgatc cagccttgcg cccgtctcat ccgtgaagag caggtccgcg ccgccggcca
     6481 ggcagtcggc gtagcggaaa cggccgttgc cgcacccgtc cgagagtttg atcagcgccg
     6541 ggaagttggt gagttccgaa acgccgtcat agccgctgaa ccgcaccggc atggagcgct
     6601 gccatgcggc caagtccggc ggcggctctt tcatcagcac cacggcgttg ccttcgacca
     6661 cggcacggta ggcgcagggc gcctcgatgt cccagccgga gaggtccgcg ctgcctgtca
     6721 gggaagcggc ctcaatgatc gtaagcctct ccgggcatgc ctcttcggaa tcggcctcga
     6781 cgcgcacggt catgaagccg ggcggcgacc aggcggtttc gatgcggatc aggtcattgg
     6841 tctgcgccgt ggcctggcag cgcagctcca tgccgttcag cgccggcgcg gcggagaagg
     6901 tcagcgtgcc gatgccgccg tcgacatccg gattgcccgg gacgaggacc gaaccgggca
     6961 gcaaagccag agacgaatac cccgcgagac ggccactgcc gcccagcacc gccccggcct
     7021 tcacggtcac gttccccgct cccagataac ccgcgtccgt gccgttcacc agcacgctgc
     7081 cgccttcgac ggtcaggccg cccgtaaagg tgttggccgc ggaaaaggcc cagacgccgc
     7141 tggtgatctt cacaatcaga ctgttcgcgc cgccgggcgc gttggcgatc acgccgttga
     7201 ccgtgttgac accctttgag gttccggcca acgtaagggt tgctgtaatg cccccggggg
     7261 cttccatggc cgaccagttg ccgttgaagg cgcctcccgc gtccgcgggg ctgttgttct
     7321 cgatcgtcac gccccgcaga aagaggaagg ggcggtctgt ggaaaagggc tccgtgccgg
     7381 tgtagcgcat cccgtagtat ccgcctgtgt agtaattccc gagccggatc gcggcgtccg
     7441 cgccaatcgc ggcacccagc gaactgggca cgccggcgtt ggccagcctt gtgacctccg
     7501 tgatcccgta tttgagcagc gccggacccg tgaaggtgtt ggagggacag gcgaggttca
     7561 aggttccctg aaagctcgtt cccattccgg ccgtcacgcc gcccgcgccg gagagcaggc
     7621 cgttcatgtt cagcttcccg tcgttcctca cggaaaagag gtgggggccc tcgggcagga
     7681 cggtgtccag cgcccagtcg actgtgccgg atgtgaaact catgaagtcc gttttgagcg
     7741 aaaccggatt acccagaaag ctccaggtat tgtcccgaac caagacatgg tggagggtca
     7801 ggccggtcag gtcattcatc gaggcgcggc gcgcgctccc gacgaacgcg agcgccggtt
     7861 cagcgggcag ggcctgctgc cagttgtccg ccgtactcca aaagtggtcc gaacccgccc
     7921 ccgaccagac cggaccatcg aacccttccg gcgtcacgcc ataacgcgcg gccagatacg
     7981 cctccacctg ctgccggtcc gtcgcggaca gggcgctctc atacacgatg acctcggaga
     8041 tcagcgcgtc gctgggatct tgcgatatgg aacccgcgcc gccgatggta agccgtcccg
     8101 gacccgtggt ccgcgtggcg tcggaggtca gatcataccc gttcacgaaa aagcgctgtt
     8161 ccgtcaaggt gttgaccgcc gcaagcgtgt aaacgcggtt gaaggtgaaa accgacttca
     8221 gacagcttga caagtcggtc atcgcgtgtt gcgcatgcgc gtaaaaggtg ccgccgtccg
     8281 gccaggtccc cagtagccag ttcgcgtcgc gcgcgggcac gacacgacgc caggtgaagc
     8341 cgcgctcgct ggcgtccacc acctgcgcca cgacgaaaac cgagtaggcg cc
//
LOCUS       DAOSRB010000122         8370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8057_length_8370_cov_5.616115, whole genome shotgun sequence.
ACCESSION   DAOSRB010000122 DAOSRB010000000
VERSION     DAOSRB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_8370_cov_5.616115"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..979
                     /locus_tag="PLZ74_07550"
     CDS             41..979
                     /locus_tag="PLZ74_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98213.1"
                     /translation="MANVGGNNGVHLTGTGNVYEICGGTGPDKSVSVRGMDNVYRLSS
                     GIQNAATITTGNMSNRVEITGGVSLAKLVMDGTNNAAWVTGGAVTGTVSFAGRDNCVT
                     VTGGEVNLASWCDGLRFENGSSNSVFCVSNTTVVKSGSFSRNWSSEGILYPFTNCPNC
                     ALEFRGAQPLFKVTSAKVSSTADPYPGVALGLDGAEMEHPLRLRYVVPDAGYETAPFQ
                     NVAGSNHNVELYDNAVFEVVLASGERPQTTLMVPLLYDQGGFWNRKHSKSSIDIEGLN
                     AHAILPFPELGTRLIRENNTVFVRIPSGRGTMVLFR"
     gene            1047..3152
                     /locus_tag="PLZ74_07555"
     CDS             1047..3152
                     /locus_tag="PLZ74_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98214.1"
                     /translation="MACTSMAEVVLKQGCVWVDDVVGVGDVRFKFSGGKASPVSCTFT
                     ENVGAVAYTDADLKATVSNGGDRVRWDFFYTVSTNARADGVMLDLRLEKGVKKKGVEK
                     HGTWRRCAANPEQGEAYETYNVKLKQMSGARGTLWYELTGNENWCGPHSEHLNFTGGT
                     NGVWRAGLTFYRAAADDPGWQVAARHAGQGMAVHLDTEKRNNLWEKGPVSMMLNVANT
                     GREAADIPVGVDVRDWDGQLVFSQTNTYALMPGERQGLPMTVPVTARGLYFAEARAGT
                     NAFTRTNFAILPPYTFAHRESSILGMNARFGKKFSSPEEVAAELKLMQRMGVRHLRSG
                     DNRVSRSYGIIAGDHFQCPKTPCDTNNVEHRTRVAKWVANTIAKGAPTLEFGNEIGYR
                     GDLAIQHRLYDAYSTWLDALRAERTRQGGTFKIIYGTSAFRPALAKLIKDKGIFDRLD
                     GIAIHPARGFFTADDAAGGWKYLGLVRGSKKMYASFGYADKPMYYTEVYAKTKPNDGW
                     ADSYRQAGENTFLTCALAVAEGAAAMHFYQLHEGISYDEDGVNPNDMEYHYGLLMRDD
                     SPKPSFMGFVTAAEVLDGARFGRWIQPEEKADKLRGMTFDSPNGPFAILYDRTEGTET
                     RWHAPVRRVKGAPFFHYEPWFNHWRVVKKHTFRATKDVVTVTDVLGRTQTFAASNGEI
                     TLELTGAPVVVHGIDVSTK"
     gene            3177..7250
                     /locus_tag="PLZ74_07560"
     CDS             3177..7250
                     /locus_tag="PLZ74_07560"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOR98215.1"
                     /translation="MHITLRFCLAIAGVALCLGAGAALPSGDIHIRDPFVIADGEEGV
                     YRLYSSIYARKNEAADAWRHTGQGVEMYLSRDLEQWTGPRHVLELPKELNCTAVWAPE
                     VHEFNGRWYLFATLTFNGTKVPHHPGWRDHVKRGTWIFAADRPEGPYEIVKRDSVTPP
                     NWSALDGTLVVEDGRPYMVFCHEWTQVRDGEMCLMPLRDDLSDAAGDPVTLFKASSAP
                     DVKASCLVTDGPSLFRDAASGDLLMTWSTVRSNGAYCVYWTKSETGSVKGPWKNHRML
                     FPENGGHGMSFRKLVGGGDRGPLTLTLHQPNGPAGAERMRFFEIEYRDGVLRTVAAAE
                     PDLQASVPKLDLTDPLTSARAYSLEIDEHMNGTDGFFPVGVLGENFTHGFNVAVGGNY
                     VCRGVDKNTKPEDFAPQGVYGISIWGAISAQTRPWLAKIRDQDGKEVRCACLFDPKVR
                     EYIFDFARTSVSNALAVADDRIFKWEIDNEYLPPLDYSDQAVAAFRVWLETKSYKGDL
                     AKLNRAWGKDYNSFAEAEPCRLDDYAKTPGHFMDWIRFQQESFAEFLAEYYKIIQSCD
                     PKRRSVNGKDTQSSLEMQRIARTRRGNHELIGKAIAPYTDGVRGMDHYGHGDRNAYEM
                     NCYTHTIVPDDWQPGKRRGMLYGENNNHNGPGWQFAQTVWRMIPNGLKGGTFFCNGWF
                     GAWGDWASFGFINPDATNREKIYYLPRFYSAVHRTERLFTESAPVRGTPTLAILMAVR
                     DVPFGIDDNMEPWGFPINSRLRLYKHLRDAGYHVNVITYEKINPVYMSQIDGLFLCAA
                     EHFDDAEIERIRAYVKGGGRLFCDVRCGAFDEHNLPRTDNNLTDVLGVTFEGIWESHD
                     IVVDQGDVWFGSPWGNLVRADGKVRWKLTTAEVVNAQHGLYYSNKAAIWTRNRYGKGR
                     AYWTNTQMGTIRSESANDEVPAREMFRALLKDAGIVPSYTLSTDETCNFRCEKPLVDT
                     KGNVVIAVSALTYRPAAPATLDVRLPADLVRDVRKAYIGLAEGNALDDVPFTRTADGV
                     RFELPALKSAAFLYLLRDHAPLLGTAFADFGGRAAAPDEATPLLKPGATFRVNVTIDN
                     PSVRADFAGGRVQLHALKGWKVSPAQTCDPLKRGARAAFAFTLTVPAEDNPELYPNRI
                     YPLTVDLFDREGRRVAVTHTVVCVDVPKKGREVLLSDNWVSRNMPWSEWTGATYRHLS
                     VADETKGEEIKDALNATRANGTEIWALQSGDRAGRRRSCVWKGIAEPTVEFDLKSVRD
                     LTRIMFWEMRTQNRALGPVAVKVAFSENGRDYTEAKSFVMAWSQKLHDVSRYAEIRLQ
                     GVRGRYVRLAFTPNRDPKHNKNGDVALDEVYIFGLGR"
     gene            7255..>8370
                     /locus_tag="PLZ74_07565"
     CDS             7255..>8370
                     /locus_tag="PLZ74_07565"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOR98216.1"
                     /translation="MALSLLFAAAFTVESTLATGQRVVETVEIPETNHVVQFVWPRAR
                     IPKDAVSVKVTPDFARARKGEEGYFVFPNNEMGTFRLDAGRTAAHHMMMPVYGMKTPR
                     RTFLAVCTGMPYAMVAVAEAKDGAYAVSADFTRHMDDVYEDLVIEYLFLEGDDADYSG
                     IARAYRKRQLDRGACRPLAVRAKERPELAYAATNIEVRIRQGWKPVPSPVPEQTLRTE
                     PKVRCLVSFDRVKDIVRESKRQGIAGQEICLVGWVYGGHDGAYPQIFPVEPSFGGEEK
                     LKEAIRFAQDEGYQIVGHCNFRDAYLIADTWDSEYILEKRPDGTLRSETQPTWGGGRK
                     YTICPQRAYERFAVKQCAEMRALGFRGLQYNDVATCTR"
BASE COUNT         1630 a         2349 c         2777 g         1614 t
ORIGIN      
        1 cttgttgacg ttcagcgggt taaaggtgtt gcaatagcta atggctaacg tggggggtaa
       61 taacggcgtg cacttgacag gaacggggaa cgtgtacgaa atctgtggcg gcacgggacc
      121 ggacaagtcg gtttccgtgc gtggcatgga taacgtgtac cggctttctt ccggcatcca
      181 gaacgcggcc acgatcacaa cgggcaacat gtccaaccgc gtggaaatta cgggtggcgt
      241 gagcctggcg aaattggtga tggacgggac aaacaatgcc gcgtgggtga caggcggtgc
      301 ggtgacggga acggtgagct ttgcgggacg ggacaattgc gtgacggtga ctggcggcga
      361 ggtgaacctg gcttcctggt gcgatggttt gcggtttgag aacggcagtt cgaactccgt
      421 gttctgcgtg tcgaacacga ccgtggtgaa gtcgggttcg ttttcacgga actggtcgtc
      481 tgaaggtatt ctttatccgt ttacgaactg tccgaactgc gcgcttgaat tccgcggtgc
      541 gcagccgctc ttcaaggtga cgagcgcgaa agtgagctcg accgcggatc cgtacccagg
      601 cgtggcgctc ggactggacg gtgcggagat ggagcatccg ttgcgcctgc gctatgttgt
      661 gccggatgcc gggtatgaaa ccgcgccgtt tcagaacgtg gcgggcagca atcataacgt
      721 ggagctttat gacaacgccg tgtttgaagt ggtgctggcg tcgggagagc gtccgcagac
      781 gacgctcatg gtgccgctcc tctacgacca gggtggattc tggaatcgga agcacagtaa
      841 gtccagcatc gacatcgaag gcttgaacgc tcatgcgatt ctgccgtttc ctgaattagg
      901 cacaagactg attagggaga acaacacggt cttcgtgcgg attccaagcg gacgtggtac
      961 catggtgctt ttccgctagg ggttttccga tgatccattt ctcacttcgt attgaccgtt
     1021 cttgcattct ggtgacggcg cttattttgg cctgtacaag catggctgaa gtcgtgctga
     1081 agcaaggatg cgtgtgggtg gatgacgtcg tgggtgtcgg ggacgtgcgt ttcaagttca
     1141 gcggaggcaa ggcgagtccc gtgtcctgca cgttcacaga aaatgtcggt gctgtggcct
     1201 acacggacgc cgatttgaag gctacggtga gcaatggggg ggatcgcgta cggtgggatt
     1261 ttttctacac ggtgtctacc aacgcccgtg cggacggcgt gatgctggat ttgcgcctcg
     1321 aaaaaggcgt caagaaaaag ggtgtggaga agcacggcac atggcggcgt tgtgccgcaa
     1381 atcccgagca gggcgaggcg tacgaaacgt acaacgtcaa attgaaacag atgtcaggcg
     1441 cacgcgggac tctctggtac gagcttacgg gtaacgagaa ttggtgtggg ccgcacagcg
     1501 agcacttgaa ctttacgggc ggaacgaacg gcgtgtggcg ggcgggcttg accttttatc
     1561 gtgcggcggc agacgacccc ggctggcagg tggcggcgcg tcatgcgggg cagggcatgg
     1621 ccgtgcactt ggatacggag aagcgcaaca acctctggga gaagggcccc gtatccatga
     1681 tgctgaacgt ggcgaatact gggcgggagg ctgcggacat cccggttggc gtcgatgtcc
     1741 gtgactggga tggacagctt gtcttctctc aaacgaatac gtacgctctt atgccgggag
     1801 agcgtcaggg actccccatg actgttcctg tcacggcacg gggactctat tttgccgagg
     1861 cgcgcgcagg gacaaacgcc tttacgcgga cgaatttcgc gattcttccg ccctacacgt
     1921 ttgcgcatcg ggaatcttcc attcttggta tgaatgcgcg gttcgggaag aagttcagtt
     1981 cgcccgaaga ggtggccgcc gagctgaagc tcatgcagcg tatgggcgtg cgccatcttc
     2041 gcagcggtga caaccgcgtg tcgcgttcct atggaattat cgcgggcgac cacttccaat
     2101 gtcccaagac gccctgtgat acgaataacg tcgagcacag gacacgtgtg gcgaagtggg
     2161 tggcgaacac cattgccaag ggggcgccga ctttggagtt cggcaatgaa attggctatc
     2221 gtggcgacct cgcgatccaa caccgcctct acgatgccta ctccacatgg ctggacgctc
     2281 tacgtgctga acgtacgcgg cagggcggca cgttcaagat catctatggt acgagcgcat
     2341 ttcgacccgc gcttgcgaag ttgatcaagg acaaaggcat cttcgaccgc cttgatggca
     2401 tcgcgatcca tccagcgcgc ggctttttca cggcggatga cgcggctggt ggctggaagt
     2461 atcttggtct cgtgcgcggc tcaaagaaaa tgtacgcttc gtttggctac gccgacaagc
     2521 cgatgtatta cacggaggtc tatgccaaga cgaagccgaa tgacggctgg gccgattcat
     2581 atcgtcaggc gggcgagaac accttcctca catgtgcgct tgccgtggct gagggtgccg
     2641 ccgcgatgca tttctaccag ttgcacgaag gcatcagtta cgatgaggac ggtgtgaatc
     2701 cgaacgacat ggagtatcac tacgggttgt taatgcgtga cgattcgccc aaaccttcat
     2761 tcatgggatt cgtaacggcc gctgaagtgt tggatggtgc gcgtttcgga cggtggatcc
     2821 agcctgaaga gaaggctgat aaactgcgcg gtatgacgtt tgattcaccc aatggtcctt
     2881 ttgcgattct ctacgaccgc acggaaggca cggagacgcg ttggcatgcg cccgtgcggc
     2941 gcgtgaaagg tgcgccgttc ttccactacg agccctggtt caaccactgg cgcgtggtga
     3001 agaagcacac gtttcgcgcc acaaaggatg tcgtgaccgt gacggacgtt ctcgggcgca
     3061 cacagacatt cgccgcctcc aatggggaaa ttacgcttga gttgacgggt gctcccgtgg
     3121 tggttcatgg gatcgacgta tccacaaaat aaggttaaag aagacgaggg ctttcgatgc
     3181 atattacact gagattctgt ctggcgattg ccggtgtggc gttatgtctg ggggctgggg
     3241 ccgcgctgcc gtcaggcgac atccacattc gcgatccgtt tgtcatcgcg gatggcgagg
     3301 agggcgtcta ccgcctctat tcgagcatct acgccagaaa aaacgaggcg gcggacgcct
     3361 ggcgtcatac gggacagggc gtggagatgt atctgagccg cgatctcgag cagtggacgg
     3421 gaccgcggca tgtcctggag ctcccaaagg agctgaactg cacggcggtc tgggcaccgg
     3481 aggtacatga gttcaatggg cgctggtatc tgttcgcgac gctcaccttc aacggcacga
     3541 aggtgccgca tcatcccggt tggcgtgacc acgtcaagcg cgggacctgg atcttcgccg
     3601 ccgaccgtcc cgaaggcccc tacgagatcg tcaagcgcga ctccgtcacg ccgccgaatt
     3661 ggagcgcgct cgacgggacg ctcgtggtcg aggacgggag gccctacatg gtcttttgtc
     3721 acgagtggac gcaggttcgc gacggggaga tgtgcctgat gccgctcaga gacgatctct
     3781 cggatgccgc aggagacccc gtcacgctgt tcaaggcctc gtccgctccg gatgtgaagg
     3841 cgagttgcct cgtgacggac gggccctctc tgttccgcga tgcggcgtcg ggtgatctgc
     3901 tgatgacatg gtcgacggtc cggtcgaacg gagcctactg cgtctactgg accaagtccg
     3961 agacggggag cgtgaagggc ccctggaaga accatcggat gctcttcccc gagaacggcg
     4021 ggcatggaat gtccttccgg aaactggtgg gcggaggcga tcggggaccc ctgaccctga
     4081 ccctgcacca gccgaatggc ccggccgggg ccgaacgcat gcgcttcttt gaaatcgaat
     4141 accgcgatgg cgtcctgcgt acggttgcgg ccgccgaacc ggacctgcag gcgtcggtgc
     4201 cgaagctgga cttgaccgat ccgctcacgt cggcacgggc gtattcgctt gagatcgacg
     4261 agcacatgaa tggcacagac ggcttcttcc cggtcggcgt gctcggcgag aactttaccc
     4321 atggcttcaa cgtcgcggtt ggcgggaact acgtctgccg cggcgtggac aagaatacca
     4381 agcccgagga cttcgcgccg cagggtgtct acggcatttc gatctggggc gccatcagcg
     4441 cccagacgcg tccatggctt gccaagatcc gtgaccaaga cggcaaggag gtgcgctgcg
     4501 cctgcctgtt cgatcccaag gtgcgtgagt atatcttcga ctttgcgcgc acgtccgtgt
     4561 cgaacgcgct cgctgtcgcc gacgaccgga tcttcaagtg ggagattgat aacgagtacc
     4621 tgccgcctct cgactattcg gatcaggcgg tcgcggcttt ccgcgtgtgg ctcgagacga
     4681 aaagttacaa gggggatctt gcgaagttga accgggcctg gggcaaggac tacaactcat
     4741 tcgccgaggc ggagccgtgc cggcttgatg attatgcgaa gacgccgggg cacttcatgg
     4801 actggattcg ctttcagcag gagagctttg cggagttcct cgccgagtac tacaagatca
     4861 ttcagtcgtg tgaccccaag cgtcgcagcg tgaacggcaa ggacacgcag agttcgctcg
     4921 agatgcagcg cattgcccgc acaagacgcg gcaatcacga gctgatcgga aaggcgatcg
     4981 cgccctatac cgacggcgtt cgcggcatgg accactacgg acacggcgac cgcaacgcct
     5041 atgagatgaa ctgttacacg cacaccatcg tcccggacga ctggcagccg ggcaaacgcc
     5101 gtggtatgct ctacggcgag aacaacaacc ataacggccc gggctggcag ttcgcccaga
     5161 ccgtctggcg gatgatcccg aacggcctga agggcggcac gttcttctgc aacggctggt
     5221 tcggcgcgtg gggtgactgg gcgagtttcg gcttcatcaa tcctgacgcc accaaccgcg
     5281 agaagatcta ctatctgccg cgcttctatt cggccgtcca ccgcactgag cgcctgttta
     5341 ccgagtcggc acctgtccgt ggaacaccga cgctggcgat cctgatggcc gtgcgcgacg
     5401 tgccgttcgg catcgacgat aacatggagc cgtgggggtt cccgatcaat tcgcgcctgc
     5461 ggctctacaa gcacctgcgg gacgcgggct atcacgtgaa cgtcatcacg tacgaaaaga
     5521 tcaatccggt ctacatgagc cagatcgacg gcctcttcct ctgcgcggcc gagcattttg
     5581 acgatgcgga gattgaacgt atccgcgcct acgtgaaggg tggcggccgg ctcttttgcg
     5641 acgtccgctg cggggccttc gatgagcaca acctgccgcg gaccgacaac aacctgacgg
     5701 acgttctcgg cgtcacgttc gagggcatct gggaatcgca cgatatcgtc gtcgatcagg
     5761 gcgacgtgtg gttcggctcg ccgtggggca acctcgtgcg ggctgacgga aaggtccgct
     5821 ggaaactgac gacggccgag gtcgtcaacg cgcagcacgg gctctactac tccaacaagg
     5881 cggcgatctg gacgcgcaac cgctacggca agggacgggc gtactggacg aacacgcaga
     5941 tggggacgat ccgcagcgag tccgctaacg acgaggtgcc cgcgcgcgag atgttccgtg
     6001 cactcctgaa ggatgcgggg attgtgccgt cgtacacgct gtcgacggac gaaacgtgca
     6061 atttccgctg cgagaagccg ctcgtggata cgaagggcaa tgtggtgatc gccgtatcgg
     6121 cgctcaccta ccggccggcc gcgccggcga cactggacgt gcggcttccg gcggatctcg
     6181 tgcgggatgt ccgcaaagcc tacatcgggc tggccgaggg caacgcgctt gacgacgtgc
     6241 cgttcacgcg cacggccgac ggcgtccgat tcgaactgcc ggcgttgaag agcgcggcgt
     6301 tcctgtatct gctgcgcgac catgcgccgc ttctggggac cgccttcgcg gactttggcg
     6361 gacgcgccgc cgcgccggac gaggcaacgc cgctgctgaa gccgggggcg acgttccgcg
     6421 tgaacgtgac gattgacaat ccgtccgtcc gcgccgactt cgccggtggt cgcgtgcagc
     6481 tccatgcgct caagggctgg aaggtctcgc cggcgcagac gtgcgatccg ctgaagcgtg
     6541 gcgcgcgcgc cgcatttgcg ttcacgctca cggtgccggc ggaagacaat cccgaactct
     6601 atccgaaccg gatctatccg ctcacggtcg atctcttcga ccgcgagggg cgtcgtgtcg
     6661 cggtgacgca tacggtggtc tgcgttgacg tgccgaagaa gggccgcgag gttctccttt
     6721 cggacaactg ggtgagccgg aacatgcctt ggagtgagtg gacgggagcg acctaccgtc
     6781 atctttctgt cgccgacgaa acgaagggtg aggagatcaa ggatgcgctt aacgcgacgc
     6841 gcgcgaacgg tacggagatt tgggcgctgc agagcgggga ccgggcggga cgacgccgga
     6901 gctgtgtctg gaaggggatc gccgagccga cggtcgagtt cgatctcaag agcgtgcgcg
     6961 atctgacgcg gatcatgttc tgggagatgc gcacgcaaaa ccgcgcgctc ggcccggtcg
     7021 ctgtgaaggt cgccttttcg gagaacggcc gcgactacac cgaagcgaag tcgttcgtga
     7081 tggcgtggag ccagaagctt cacgatgttt cgcgttacgc cgagatccgg ctgcagggcg
     7141 tgcgcggacg ctatgtgcgc ctcgctttca cgccgaatcg cgacccgaag cacaacaaga
     7201 acggggacgt ggcgctggac gaggtctaca ttttcggatt agggaggtaa gggaatggct
     7261 ttgagtttgt tgtttgctgc ggcatttacg gtggagtcga ctctggcgac gggccagagg
     7321 gtggtcgaga ccgtcgagat acccgagacg aaccatgtcg tgcagttcgt ctggccgcgg
     7381 gcgcgcatac cgaaggacgc ggtctcggtg aaagtgacgc cggatttcgc gcgggcgcgg
     7441 aagggtgagg agggctactt cgtattcccc aacaatgaga tgggaacgtt ccggctggac
     7501 gcggggcgga cggctgccca tcacatgatg atgccggtct acggtatgaa gacgccgcgg
     7561 cggacgttcc ttgccgtctg cacggggatg ccgtatgcca tggttgccgt tgcggaggcg
     7621 aaggatggcg cttatgccgt ttcagcggat ttcacacgcc acatggacga cgtctatgag
     7681 gatctcgtga tcgaatatct gttcctggaa ggcgacgatg cggactactc aggtatcgct
     7741 cgcgcctacc ggaaaaggca gctcgaccgc ggcgcgtgcc gtccgctggc cgtgcgggcc
     7801 aaggaacgtc ccgaactcgc ctacgccgcg acgaacattg aggtccgcat ccgccaggga
     7861 tggaaacccg tgccgtcccc cgtgcccgaa cagacgttgc gaacggaacc gaaggtgcgt
     7921 tgtctcgtct cgttcgaccg cgtgaaggac atcgtccgcg aatcgaaacg gcagggtatc
     7981 gccggacagg aaatctgcct cgtgggctgg gtctacggcg gacacgacgg cgcgtatccg
     8041 cagatcttcc ccgtcgaacc gtcattcggt ggcgaggaga agctcaagga ggcgatccgg
     8101 ttcgcccagg atgagggcta ccagatcgtc ggtcactgca acttccggga cgcctacctg
     8161 atcgccgaca cctgggacag cgaatacatc cttgagaagc gtcccgacgg tacgctccgc
     8221 agcgagaccc agccgacctg gggcggcgga cgcaagtaca cgatctgtcc gcagcgcgcc
     8281 tacgagcgct ttgccgtcaa gcagtgcgcc gagatgcgcg cgctcgggtt tcgcggactc
     8341 cagtacaatg acgtcgccac atgcacgcgg
//
LOCUS       DAOSRB010000123         8278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8178_length_8278_cov_6.881430, whole genome shotgun sequence.
ACCESSION   DAOSRB010000123 DAOSRB010000000
VERSION     DAOSRB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8178_length_8278_cov_6.881430"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="PLZ74_07570"
     CDS             <1..1238
                     /locus_tag="PLZ74_07570"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOR98217.1"
                     /translation="MMATSSLTPAYEKVDELDDLPCHVNAAICNAPAYATGLGAAGGA
                     APQDGTLVPYPTINPCFKFDAKTCPISFHHGAVDPYSPNGSTLCYREVRKRSVPSELH
                     LYADLGHGAHGLERAVEFLRQLNFDGRLGAEEERRIFTGTHTAEQVCEKLWPEGRVPS
                     HSAAQTNTATLTWFVPKTLKTKAIQVIFPGGAYTHCNVRGEGRPVAEYFNAQGMTAVV
                     VEYRCPRPKNLPKHWTAWQDAQRAIRLVRAGAVARGLDPERIGAMGFSAGGHLTLMTA
                     LSSTYPAYNPVDDVDKKVSCRVAWACPIYPAYVLSDGADGAHARGGNDDKATLVPELL
                     FDVNTPPMCFVHGDADSITAMGSVKVWEKLRRMGASSDLHTLATRGHCFQFKAAPGTG
                     SWTWLDQVWDFLVRKGFVK"
     gene            1263..3554
                     /locus_tag="PLZ74_07575"
     CDS             1263..3554
                     /locus_tag="PLZ74_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98218.1"
                     /translation="MMKRVFSLALMSAAAPLFAATYYASPIGTSSAAGTEADPYDIAT
                     AIAKLGNNTYDDVYLLPGTYKVGSASLAAIDYYARLIGKGATRDDVVLDFEGAGTALK
                     VQTGASVCNLTISNFVNCSAMEIINYNGYGELIVSNCVIRSGSGTKPGMTMTGAGPIL
                     VVDSVIRDIDGNISGGGYGVGVNCSAAGACFRRCTFANNTYTGWVGLGGAAGYFSAAA
                     LVENCIVSNNSATGYGGGFHTTTGMRIKDTLFIGNSSSANGGGAIYSQSGANNTNFIV
                     GCTFIDNRTTVSSVKGGAIFAGFYNTLVSNCSFTANASLSNGGAIHCYDSATNKTIGI
                     EIVGCGFTNNTASSGGSAITIGRRGAERIEPPFNIRNCHFKGNTNSANATGGGIQLYG
                     FVQANVEDCDFEDNQAGVSSGEGGAIGRTNDAWNEAMARAVCRRCRFVNNRAAVGAAI
                     AATASLYDCDFFGNIATNSNASGCNAIVYLYGKRLVAKEYLDEAFTNFVSGCHFEGNV
                     VYGTTMGIMAPFGDFRCYDTTFSNNVGYTSHITGGWSVNLRAPTYFEVDRCQFVGNTA
                     ASSGSGFHCEKGTMNDSMTNGWVRNSLFHKNYSVTSGGAACGMIQLGWTNTWISGCTI
                     VSNYIYGGIWAAVNTSCTQSNCVANCIMYMNDTNSGTQYRDVNGSADKQPYFHNCFIS
                     NGKFTDNGCLGKDQDPGFVDIASGNLQLRRDSVCVDKGDNEPWMFAGKIPDLAGRPRI
                     SGSSVDIGCYEYCPILRFILSIR"
     gene            3643..8277
                     /locus_tag="PLZ74_07580"
     CDS             3643..8277
                     /locus_tag="PLZ74_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOR98219.1"
                     /translation="MKMCVYAAAVLAVGAVFAAEQKTGEPEAKISIYADRPEFLYGAD
                     EKVEFVVKATSADGAPFTNGVLEVILDNYGGRGLFREKITDEVKLAKPVTVRADPLGK
                     PGFMRCRVLYRSPSGKETWEYAAVAVDPRAITSGAVRPKDFDAFWDGEIVRLEKEVPD
                     GVTAVKDEKASTETHTCFRMCAKVFGERSVWGFLTVPNGAFAAEPKRYPVKINVPGAG
                     VGVYWFPAQKQRGEAITLTINVFDFEPGWETGRDWKIHYERYHDMHRRWEQKGGLREK
                     SQSWAAGFGQSRTNTFYYGAILGANRMVNWLCSQPYIDKKDVAYRGQSQGGAFGIFLA
                     SLNTNLTAATISEPALTGLTAEDEDKHQPGWPNPINMQTNDAAKKIAREVAPYFDGAH
                     FAPRIKCPVRFAVGYIDLLCPPHCVYAAYNALKPTVDRRIVLGPGVGHGCPKEPYQDM
                     EQRLTDSWKKAAPYAWAEEFFNPDHPGLTDRYPYPDRTAPSTYLYVAPDGDDGAEGTL
                     EKPLRTMEGARDRLRKLTLSGRRHLPRGGGVVFFRSGIYVVTNTIEFTKNDSAHFKWS
                     PIVYRAYPGEKPVFTGGLEFSGTAELKAPEKLSQLPEGSKGKVKVIDLKAKGFDDFAP
                     QAAFGYRPKKPCHTVTDLYRDGKPLDVAVSPNPDIRLEDYYRIGEVLDKKHDVFKADF
                     PDMDKWAKEEDVMAQGWWTFLWASTTIPVEKIDPVAKTVTVAREPNIFYYAATSNNVF
                     TLENGLPALDRENEWVFDRKNGLAYVLEPKGAKAPVYTVTRLAKPMITVTDVNNFRIE
                     GLTFEYGRTDAIIVSNAVNFAFLGNTVRNFGGRGLQVYEAKEARVQNNTFHHFGFAAL
                     ILTGGDRPTLTPGMCIIENNDISFTGRRQRTSPESSTCQVGGCGNVLTHNWFHDTPGS
                     ALRLAGNDHRVSFNRFERCSFMSDDNGAIDMCNNMSYAGNRFVFNEFYDIGYKNPRCG
                     NAGIRLDDFISQQYLYGNRFVNASGGKFGAIQLNGGRDNISENNWIEGSQWAFSVDGA
                     VGAWWNRNQTMDYNVKKYSGDVSVYDEPYISRFPRTPEIIREAHSCTNTFRRNVIIGA
                     AALKEKRTWHQTVFEDNWMFDKIPTGEELAKTKFKPIPPAGSAGIYEIDKEPEIARPP
                     MIEQKFRDGIGRTFRKLQKGGDVRIGFFGGSITAMYGWRNFILKKLAEDFPQANIIEV
                     NASLGGAGSLSGAFRMRHAILGKKCDLMIIEYATNDTENSPAMNLRSMEGCVRQAWNS
                     DPEMDLLFVYTMNMSQFETYKLGLTSDTAYIHDLIADHYGIPSVNFCPRLMEEINSGR
                     WVYMAADIRNKFGIKPGEPDYEKRFEEAKKKDGRTIFTFDNCHPSADGHKLYHEILAK
                     AFDDMRARNDPPANHAAKMVLPPLAADNMEKAKLVFPDETIFSKGDWSILPKDHKESK
                     KFQRETDRIWFTDKIGAKIRFRFRGRAVQMYNIRGPKSCAIKVSVDGNPPFTRVLADP
                     WSAGSTLIWSEKIFNGCDSIHTVEIEIMRNPKHGGTELELGGLQLDGELL"
BASE COUNT         1750 a         2417 c         2508 g         1603 t
ORIGIN      
        1 tgatgatggc gacgagttcc ctgacgcccg cgtacgagaa ggtggacgaa ttggacgatc
       61 tgccctgtca tgtcaatgcc gcgatctgca atgccccggc gtatgcgacc ggacttggcg
      121 cggcgggcgg tgcggcgccg caggatggca cgctcgtgcc gtatccgacg atcaatccgt
      181 gtttcaagtt cgacgcgaag acatgtccga tcagcttcca ccatggcgcg gtcgacccgt
      241 attcgccgaa cggctcgacg ctttgctacc gcgaagtgcg taagcggagc gtcccgtctg
      301 aactgcacct ttacgcggac ctcggccatg gggcgcacgg acttgagcgc gcggtcgagt
      361 ttctccgcca gctcaatttc gacggacgcc ttggcgcgga ggaggaacgg cgcatcttca
      421 cgggtacgca cacggccgag caggtttgcg agaagctgtg gcccgaggga cgcgtcccgt
      481 cccattcggc cgcccagacc aatacggcga ccctcacttg gttcgttccg aaaaccttga
      541 agacgaaggc catccaggtg atttttccgg ggggcgccta cacgcactgt aacgtccggg
      601 gggagggacg tcccgttgcc gaatacttca atgcgcaggg gatgacggcg gtggttgtcg
      661 agtaccgctg tccgcgaccg aagaatctgc cgaagcactg gacggcttgg caggatgcgc
      721 agcgggcgat ccgccttgtc cgggcgggag ccgtggcccg cggactcgac ccggagcgca
      781 tcggcgcgat gggcttttcg gcgggcggac acctgacgct catgacggcg ctcagttcaa
      841 cgtaccctgc gtacaatcca gtggatgatg tcgataaaaa ggtttcctgc agggtcgctt
      901 gggcatgtcc gatctatccc gcgtacgtgc tttcggatgg cgctgacggt gcccatgcgc
      961 gaggcgggaa tgacgataag gcgacactcg tccccgagct tcttttcgac gtcaatacgc
     1021 cgccgatgtg ctttgtgcat ggcgatgccg actccattac ggcgatggga tccgtcaagg
     1081 tctgggagaa actccgccgg atgggcgcgt cgagtgatct tcatacgctc gcgacccgtg
     1141 ggcactgttt tcagtttaaa gcggcacccg gtaccggcag ctggacctgg cttgatcagg
     1201 tgtgggactt ccttgtccgc aaggggttcg tgaagtgaaa tccgaataac aggagaatgg
     1261 acatgatgaa acgtgttttt tcactggcac tgatgtccgc ggccgcgccg cttttcgcgg
     1321 cgacttacta cgcttcgccg atcgggacgt cctcggccgc aggtacagag gcggatccgt
     1381 acgatatcgc gacggcgatc gccaaactcg gtaacaacac ctatgatgat gtctacctcc
     1441 taccgggaac ctataaggtc ggcagcgcct cgctcgcggc catcgactac tacgcgcggc
     1501 tgatcggcaa gggcgcgaca cgcgatgacg tggtcttgga cttcgagggc gcgggtacgg
     1561 ctctcaaggt tcagaccggc gcaagcgtct gtaatctcac gatctccaac ttcgtcaact
     1621 gttcggccat ggagatcatc aactataacg gctacgggga actgatcgtt tccaactgcg
     1681 tgatccgctc cggctcgggt accaaacctg gaatgacgat gaccggagca gggcctatcc
     1741 ttgtcgtcga cagcgtcatc cgtgacatcg acgggaacat ttccggaggc ggttatggtg
     1801 tgggagtcaa ttgctcggct gctggcgcat gcttccggcg ttgcacgttc gccaacaaca
     1861 cctataccgg gtgggtcggt ctgggcggtg ccgcgggata tttttccgct gcggcgctcg
     1921 ttgagaattg catcgtctcc aacaacagtg caacgggcta tggcggagga ttccacacca
     1981 ccacgggtat gcggatcaag gacacgcttt tcatcggcaa ctcttcgtct gcgaacggcg
     2041 gcggcgccat ctacagccag agcggcgcga acaacaccaa ctttattgtc ggctgcacgt
     2101 ttatcgacaa tcgcacaacc gtgtccagcg tgaagggcgg ggcgattttc gcgggttttt
     2161 acaatacgct cgtctccaac tgcagcttca cggcgaatgc ttcgctcagt aacggcggcg
     2221 ccattcactg ctacgactcc gccacgaaca agacgatcgg catcgagatc gtcggctgtg
     2281 gcttcacaaa caacaccgcg tcgtcgggcg gcagcgcgat cacgatcgga cgacgcggag
     2341 cggaacgcat tgagccgccc ttcaacatcc ggaactgcca ctttaagggc aataccaact
     2401 cggccaatgc gaccggcggc ggtattcagc tctacgggtt tgtccaggcg aatgtcgaag
     2461 attgcgactt cgaggacaat caggcggggg tgtccagcgg cgagggcggc gcgatcgggc
     2521 gcaccaatga cgcttggaac gaggcgatgg cccgcgcagt ctgccggcgt tgccggttcg
     2581 tgaacaaccg ggcggctgtg ggtgcggcga tagccgccac ggcctcgctc tatgactgtg
     2641 acttcttcgg caacatcgcg accaattcca acgccagcgg ctgcaacgcg attgtttatc
     2701 tctacggcaa acgcctggtc gccaaggaat atttagacga ggccttcacg aatttcgtat
     2761 cgggttgcca tttcgaagga aacgtcgtct acggaaccac catgggcatc atggcgccct
     2821 tcggtgattt ccgctgttat gacacgacct tctcgaacaa tgtcggctac acgagccaca
     2881 ttacgggtgg ctggtcggtc aatttgcgcg cgccgaccta tttcgaggtg gaccgctgtc
     2941 agtttgtcgg gaacaccgct gcgtcgtccg gatccggctt ccactgcgag aagggaacga
     3001 tgaacgacag catgacgaac ggttgggtcc ggaattcgct tttccacaag aactattccg
     3061 tcacttccgg cggcgcagcc tgcggcatga tccagctcgg ttggacgaac acgtggatct
     3121 cgggctgtac cattgtctcc aactacatct acggcggcat ctgggctgcg gtcaacacga
     3181 gctgcaccca atcaaactgc gtcgctaact gcatcatgta catgaacgac accaacagcg
     3241 ggacgcagta ccgggatgtg aacggcagcg cggataagca gccgtatttc cacaactgtt
     3301 tcatcagcaa cggcaagttc acggataatg gatgcctcgg caaagatcag gatcccggat
     3361 tcgtcgacat tgccagtggc aatctccaac ttcgcaggga ttcggtgtgt gtggataaag
     3421 gcgacaacga gccttggatg tttgcgggaa aaatccccga tctcgcagga cggccccgta
     3481 taagtggttc ttccgtcgac atcggctgct acgaatactg cccgatcctt cgtttcatcc
     3541 tgagcatccg ctaaacgttt caaaccgtct agcccttccg agcctcatca acatcatctc
     3601 gcgaagatgg gggaacggtc ggctaatgga aaaggaaatc gaatgaagat gtgcgtttat
     3661 gcggcagcgg tgctggccgt gggggcggtg tttgccgcag agcagaagac cggggaaccg
     3721 gaggccaaga tcagcattta cgcggatcgt ccggagtttc tctacggcgc ggatgagaag
     3781 gtcgagtttg tcgtgaaggc tacgtctgcg gacggagcgc cgttcacgaa tggcgtgctg
     3841 gaagtcatcc tcgacaacta cggagggcgc ggtctttttc gcgagaagat cacggacgag
     3901 gtgaagcttg cgaaacccgt gaccgtaagg gcggatcctc tcggtaagcc gggtttcatg
     3961 cgttgccgtg tgctctatcg gtcgccgtcc ggaaaggaga cgtgggaata tgccgccgtc
     4021 gcggtcgatc cgcgtgcgat cacgagcggt gcggtacggc cgaaggactt cgacgcgttc
     4081 tgggatggcg aaatcgtgcg gcttgagaag gaggttcctg acggcgtcac ggccgtgaag
     4141 gatgaaaagg cctccacaga aacgcacacc tgctttcgaa tgtgcgcgaa ggtgttcggc
     4201 gagcgcagcg tttggggttt tttgaccgta ccgaacggcg cgtttgccgc agagccaaag
     4261 cggtacccgg tgaagatcaa cgtgccgggg gcgggtgtgg gcgtctactg gttccccgcc
     4321 cagaaacagc gaggcgaggc gatcacgctt acgataaacg tcttcgactt cgagcctggc
     4381 tgggaaacgg ggcgcgattg gaagatccac tacgagcgct atcacgacat gcaccgtcgc
     4441 tgggaacaga aaggcggact ccgcgagaag tctcagagtt gggcggcggg cttcggccag
     4501 agccgcacga acacgttcta ctacggcgcg attctcggcg cgaaccgtat ggtcaactgg
     4561 ctctgctcgc aaccgtacat cgacaaaaag gatgtcgcct accgcggaca gagtcagggc
     4621 ggcgcgttcg gtatcttcct cgcctcgctt aacacgaacc tcacggcggc aacgatctcc
     4681 gagccagcgc tcacgggact caccgccgag gacgaggaca agcaccagcc cggatggccg
     4741 aatccgatca acatgcagac aaacgacgcc gcgaaaaaaa ttgcccgtga ggtcgcgccg
     4801 tatttcgacg gcgcccactt cgcgccgcgc atcaagtgtc cggtacgctt cgcggtcggc
     4861 tacattgacc tgctctgccc accgcactgc gtctacgcgg cctacaacgc gctgaagccg
     4921 accgtcgacc gccgcatcgt ccttggcccg ggtgtcggac acggctgtcc aaaggaaccg
     4981 taccaagata tggaacagcg tctcaccgac agttggaaga aggctgctcc ctatgcgtgg
     5041 gcggaggagt ttttcaaccc cgatcatcct gggctcacgg atcgttatcc gtatccggac
     5101 cggactgctc ccagcactta tctctacgtc gcaccggacg gcgatgacgg tgcggagggc
     5161 acacttgaga agcccctccg cacgatggaa ggagcgcgcg accgcctgcg caaactgacc
     5221 ttgagcggac gcagacacct tccccgcggc ggcggagtcg tctttttccg cagcggcatc
     5281 tatgttgtca ccaacacgat cgaattcacc aaaaacgact ccgcgcactt caagtggagt
     5341 ccgatcgtct atcgcgcgta tccgggcgag aagccggttt tcaccggcgg cctggagttt
     5401 tcggggacgg ccgaactgaa ggcccccgag aaactctcgc agcttcctga gggctcgaaa
     5461 ggcaaggtca aggtcattga tctcaaggca aagggatttg acgacttcgc gccgcaggcg
     5521 gcgttcgggt atcgcccgaa gaagccgtgc cacacggtca ccgacctcta ccgcgacggc
     5581 aagcccctcg atgtggcggt gtcgccgaac cccgacatcc gtctcgaaga ttattaccgc
     5641 atcggcgaag tgctcgataa gaaacacgat gtcttcaagg cggactttcc tgacatggac
     5701 aagtgggcga aggaggagga cgtgatggcg cagggttggt ggaccttcct ctgggcgtcg
     5761 acgacgattc cggtcgagaa gatcgacccc gttgcaaaga cggtcaccgt cgcgcgcgag
     5821 ccgaacatct tctactacgc cgccacgtcc aacaacgtct ttacgcttga gaacggcctt
     5881 cccgcgctcg accgcgagaa tgagtgggtg ttcgaccgca agaacggtct cgcctacgtg
     5941 ctcgaaccca agggcgcaaa ggcgccggtc tatacggtca cgcgtctcgc gaaaccgatg
     6001 atcacggtga cggatgtcaa caacttccgc attgagggcc tgacctttga gtacggcaga
     6061 accgacgcaa tcatcgtttc gaatgcggtg aacttcgcct ttctcggaaa taccgtccgg
     6121 aacttcggcg gacgcggcct gcaggtctac gaggctaagg aagcgcgtgt ccagaacaac
     6181 acgttccacc acttcggatt tgccgcgctc atcctgacgg gcggcgaccg tccgaccctg
     6241 accccgggca tgtgcatcat tgagaacaac gacatctctt ttacgggacg ccgccagcgt
     6301 acttcgcccg agtccagcac ctgccaagtc ggcggctgcg gcaatgtgct cacgcacaac
     6361 tggtttcacg atacccccgg tagcgcgctc cgcctcgccg gcaacgacca ccgcgtctcc
     6421 tttaatcgtt tcgagcgctg ttcgttcatg agcgacgaca acggggcgat cgacatgtgc
     6481 aacaacatgt cctacgccgg gaaccgcttt gtcttcaacg agttctatga tatcggctac
     6541 aagaacccgc gctgcggcaa tgcgggcatc cgtctggacg acttcatatc gcagcagtac
     6601 ctgtacggca accgcttcgt gaacgcttcg ggtggcaagt tcggtgcgat ccagttgaat
     6661 gggggacgcg acaacatatc cgagaacaac tggatcgagg gctcgcagtg ggcgttcagc
     6721 gtagacggcg ctgtgggcgc gtggtggaac cgcaaccaga cgatggatta caacgtgaag
     6781 aagtattccg gggatgtgag cgtctacgac gaaccctaca tctcgcgctt tccgcgcaca
     6841 ccggagatca tccgcgaggc ccattcgtgc accaacacct tccgccgcaa tgtcatcatc
     6901 ggtgccgcgg cgctcaagga aaagcgtacg tggcatcaga cggtcttcga ggacaactgg
     6961 atgttcgaca agattccgac tggggaagaa ctcgcgaaga cgaagtttaa gcccattcct
     7021 cccgccggta gcgccggcat ctatgaaatc gacaaggagc ccgagatcgc gcgtccgccg
     7081 atgatcgagc agaagttccg cgacggtatt ggccgtacgt ttcggaagtt gcagaagggc
     7141 ggtgatgtga ggatcggttt cttcggcggt tccatcaccg cgatgtacgg ctggcgcaac
     7201 ttcatcctca aaaaactcgc ggaggacttt ccgcaggcga atattattga ggtgaacgcg
     7261 tcgctcggcg gcgcgggttc gctttcggga gcgttccgta tgaggcacgc catcctcggt
     7321 aagaagtgtg acctcatgat catcgaatac gcgacgaatg acacggagaa cagcccggcg
     7381 atgaacctcc gttcgatgga ggggtgcgtt agacaggcgt ggaattccga tcctgaaatg
     7441 gatctgctct tcgtctacac gatgaacatg tcccagttcg aaacctacaa gctcgggtta
     7501 acctccgata ccgcctacat ccacgacctt atcgccgacc attacggcat tccgagcgtg
     7561 aacttctgtc cgaggcttat ggaggagata aactcagggc gctgggttta catggccgct
     7621 gacatacgca acaagttcgg aatcaagccc ggcgagccgg attatgagaa gaggttcgag
     7681 gaggcgaaga agaaagacgg acgcacgatc ttcaccttcg acaactgcca tccgagcgcg
     7741 gacggccata agctctacca cgagatcctc gcgaaggcgt tcgacgacat gagggcgagg
     7801 aacgatccgc cggcgaacca cgccgcaaag atggtgcttc cgccgctcgc ggcggataac
     7861 atggagaagg cgaagcttgt ctttccggac gagacgatct tctcgaaggg cgactggtcg
     7921 attctcccaa aggatcacaa agagtcgaag aaattccagc gcgagacgga ccgtatctgg
     7981 ttcacggaca aaatcggggc gaagatccgg ttccgtttca ggggccgtgc tgtccagatg
     8041 tacaacatcc gcggaccgaa gagctgcgcg atcaaggttt ccgtggacgg caatccgccg
     8101 ttcacccgtg ttctcgccga tccgtggagc gcgggttcga cgctgatctg gtccgagaaa
     8161 atcttcaacg gttgcgattc gatccatacg gtcgaaatcg aaatcatgcg taacccgaaa
     8221 cacggcggca ccgaacttga actcggcggt ctccaacttg acggtgagct gctgtgat
//
LOCUS       DAOSRB010000124         8217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8248_length_8217_cov_6.090542, whole genome shotgun sequence.
ACCESSION   DAOSRB010000124 DAOSRB010000000
VERSION     DAOSRB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8248_length_8217_cov_6.090542"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PLZ74_07585"
     CDS             complement(<1..565)
                     /locus_tag="PLZ74_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOR98220.1"
                     /translation="MTQKKLVTLIITAVVLVGLAYLTSSSRKMKTPSLVGKPVVRTFD
                     LSEVAKIEAGSQNGAKLMLESTDTGWVIRSLFGYPADIAKIRENLLKLKDLKAGHVAV
                     GKKLENPVLVDLQNASGKSLASLRLGDKHMRQPSGEMSQFGGGGYPDGRYVAGETDDT
                     VVLVKETLDAFDGDVKSWTDTQIASVPS"
     gene            complement(651..2561)
                     /locus_tag="PLZ74_07590"
     CDS             complement(651..2561)
                     /locus_tag="PLZ74_07590"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOR98221.1"
                     /translation="MSDKQTKGFSRIGTGAAGLAALLVILGAANLIIANLRLRVDLTA
                     EKLYTLSQGSRQVLGKLENDVVLKFYFSASSADMPMGLKTYATQVQDLLKEYEIAGRG
                     RITLEAYDPKPDSDSEEWAQRYGIEPQQTNPFGQPVYFGLVAVSGDQEQVIPGFNPRT
                     EATLEYDITRLITRAAWPEKPVLGVLSSLSVLGSPQNPMMMMQRRQQQDQGWMAFREL
                     RKDYVVREVRPDTETIDNDVKTLIVVHPKDLSDQTLFAIDQFVLRGGRLVVCVDPFSI
                     ADFEANQQQNPMMMQMGGGQAGPSTLGKLFDAWGVSFDTSKVVADLSCATKLNAGNGR
                     IEENPAFLSLGTANMAKDDLLTAQLSQVMMPFAGALSANTPKEIIFTPLITTSRDHAC
                     LVDQMNAQFGMGAMRAQLKPDGAQRVLAARLHGTFKTAFPQGVGTNGTDQAAATNAAP
                     NHLTSGTSTVMVFGDTDFLNDRFCVQIINSLFGSIAQPINDNLTLFGNTVEQFAGREE
                     LIGVRSRGQFNRPFVKVDQLEVKAMRQWQAEEERLEAALQETQQRLTELQQRKSGNER
                     LILSKEQQAELEQFRKTQVETRKQLKKVRKNLNSEIERLGLFLKVLNIALVPLLVIGF
                     GVQRGLRRRGRR"
     gene            complement(2610..3356)
                     /locus_tag="PLZ74_07595"
     CDS             complement(2610..3356)
                     /locus_tag="PLZ74_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR98222.1"
                     /translation="MCNVKNIKSIFEREFKSYFDSPVAYVFLVAFLVLIGFLTFGVAM
                     FYERRQANLMPFFFWHPWVYLLLVPAATMGLWAEERRTGTIELLLTLPVTLAETLVAK
                     FLAAWAFIGIALALTFPIVLTTAWLGNPDMGAIFCGYLGSLLLSGACVAIGVFASALS
                     RSQVIGFVIALAICLFMLIIGFDPVINAVAGWGVPAGLVDAVASCSLMRHFDAMRRGV
                     IDFHDIGYYVSVILFMLAAAHVVTDNRKAS"
     gene            complement(3349..4080)
                     /locus_tag="PLZ74_07600"
     CDS             complement(3349..4080)
                     /locus_tag="PLZ74_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR98223.1"
                     /translation="MLTVSNLKKNFGPIQAVKDVSFTVEKGEVLGFLGPNGAGKSTTM
                     RMITGFIPPTAGTATICGHDILTEPVAAKACIGYLPENAPSYHTMTVTDFLTFIGKVR
                     GYTGQELKDQVESVIKKSRLETVRNQTIETLSKGYRQRTCFAQAILHDPPVLIMDEPT
                     DGLDPNQKFVVREMIKEMAADKAIIVSTHILEEVDAVCTRVIVIADGEVVANATPAEL
                     RAQDPQGRLDVVFRNLTMTEGVSHV"
     gene            complement(4207..5580)
                     /locus_tag="PLZ74_07605"
     CDS             complement(4207..5580)
                     /locus_tag="PLZ74_07605"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR98224.1"
                     /translation="MNTKPNLLIIEDDPDNARSVTEAAEDAGFATVCVANGKAGVAAF
                     RNQIPDLVLSDLVLPDMDGLEVLARLRKIDDMVPVMIMTAYGSVESGVRAMREGAYDY
                     VTKPLDLDDIQSKLRRACEISRLRRQVDKLSRSVREKYASTAILAESPGMKKVIRQIE
                     ALADTSATVLICGESGTGKELVARALHVDSKRADGPFVAVNCGAFAENLLESELFGHE
                     KGAFTGAIGMYKGAFERADGGTLFLDEIGDAPASVQVKLLRALEEKEVRRVGGKEVFS
                     VNVRLVSATNRDLDALVREGTFREDLFYRLNVVTLRIPPLRERREDIRPMTDRFIARA
                     AVENNKTITCVRPEFYAALEAFSWPGNVRQLRNVVESAVLLSPDGELDADSAALPDEK
                     ARADPDGAAPQGLTFPQDMTLEQIERAALEEGLRRNNGNRTLTADQLGLSRRTIQRKI
                     KEHNLPY"
     gene            complement(5588..6988)
                     /locus_tag="PLZ74_07610"
     CDS             complement(5588..6988)
                     /locus_tag="PLZ74_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOR98225.1"
                     /translation="MRQSDRPVNRGARGGWRLELILLSALLTSAFAAGCVLVLLKGRH
                     DRQAHLAATATVLQEGEALLQALCDLGDCSSTHPSPAEWRRFSTVVDSAFAVRKDVQS
                     VSVSRNGITVFQRQAGGLHPPQGESAESRAREKERRVETEIAPGTLEINGEPQSVFVI
                     TRTQTLDDGGRVVTEATFRREAVGAEERTARSLVSSLFWFSLAVVFLSFALCAAVLVF
                     FVARDRRREQRARREEHLAFSGVLANGILHDFRNPMSAVRLDAQMLGREMARADGFRA
                     ERVRDLAERIARTMARMDKVFQEFLFLAKPADEQPETVRIEQVVRECVDTLAPRLEQA
                     GVHVAIHAPDTMPAVSAFPFALRRALLNVLMNAIQFAPEGSEITATIEERGRHIVLDV
                     LDRGPGIPPAEREKVFEMFFSGRPGGTGLGLFLARTAVRRCGGDIQVLTREGGGADLR
                     ITLPVAPPSSPPPSDV"
     gene            7409..7936
                     /locus_tag="PLZ74_07615"
     CDS             7409..7936
                     /locus_tag="PLZ74_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98226.1"
                     /translation="MRNAKFIGRAWAALDYFLLVAVLGLLVTLAFLGRSVGEAAADGN
                     GEISGGTEPVERVRPFKSRVVEGLRVSAGRQRLDVIAQSMAASDWDRLPASPALDMRE
                     NGQAYEILFSLPSGVDRDNVRVTSSGSLLTLALTDPGTGKVLMRRIRIPCEAEPNSRI
                     HSVVSNRVLHVRIYP"
BASE COUNT         1548 a         2442 c         2583 g         1644 t
ORIGIN      
        1 ccgatggcac cgaggcaatc tgcgtgtcgg tccagctctt gacatcaccg tcaaacgcat
       61 cgagcgtttc cttaaccaga acaacggtgt catcggtctc tccggcgacg tagcgtccgt
      121 ccggatatcc gccgccgccg aattgcgaca tctcgcctga gggctggcgc atgtgtttat
      181 cgccgagacg caggctggcg agcgatttgc cggaagcgtt ctgcagatcg accagcaccg
      241 ggttctccag ctttttgccg acagccacat gtcccgcctt aaggtcctta agtttcagca
      301 ggttctctcg gattttggcg atgtcggcgg gatatccgaa gagcgagcgg atcacccagc
      361 cggtgtccgt gctttcgagc atcagcttcg cgccattctg ggaaccggcc tcgatcttcg
      421 cgacttcgct gagatcaaag gttcggacca caggcttgcc caccagcgaa ggggttttca
      481 tttttctgct ggagctggtg aggtaggcga gtccgaccag cacgaccgcc gtaatgatga
      541 gggtgacgag tttcttttgt gtcatgatac gaatccttgt tggagaggtt acgggacaaa
      601 agggatgcgt cgggaagccg ggacggctgt gccgccccgg ccccgtcgga tcagcggcgc
      661 cctctacggc gcagtccgcg ctggactccg aagccgatta ccagcaacgg caccagggcg
      721 atgttcagga ctttcaggaa caggcccaga cgctcgattt cgctgttcag gtttttgcgc
      781 acctttttga gctgcttgcg cgtctcgacc tgcgttttgc ggaactgctc cagttcggcc
      841 tgctgctctt tcgagaggat caggcgctca ttgccgctct ttctctgttg cagttcggtc
      901 agacgctgct gcgtctcctg caacgcggcc tcaagacgct cctcctcggc ctgccactgc
      961 cgcatggcct tcacttcgag ttgatccacc ttgacgaacg ggcgattgaa ctggccacgc
     1021 gaccgcacgc cgatcagctc ctcccgtccg gcgaactgtt caaccgtgtt gccgaaaagg
     1081 gtcaggttgt cattgatcgg ttgcgcgatg gagccgaaca gcgagttgat gatctgcacg
     1141 cagaaacggt cgttcaggaa atcggtgtcg ccgaaaacca tcaccgtgct ggttcccgaa
     1201 gtcagatgat tcggcgcggc attggtggcg gcagcttggt ccgtgccgtt cgtgccgacc
     1261 ccttggggga aggccgtctt gaaggtgccg tggaggcgtg ctgccagcac gcgttgcgcg
     1321 ccatcgggtt tgagctgggc gcgcatggca cccatgccga actgggcgtt catctgatcc
     1381 accagacagg cgtgatcgcg ggaggtggtg atgagcggcg tgaagatgat ctccttgggc
     1441 gtgttggcgg acagcgcgcc cgcaaagggc atcatcactt gggacagttg cgcggtcaga
     1501 agatcgtcct tggccatgtt cgcggtgccc agcgagagaa aggccggatt ttcctcgatg
     1561 cgcccgttgc ctgcattgag tttcgttgca caactcaagt cggccacgac cttggaggtg
     1621 tcgaagctga cgccccaggc gtcaaacagt ttgccgaggg tggagggacc ggcctgtccg
     1681 ccgcccatct gcatcatcat cggattctgc tgctggttag cctcgaagtc cgcgatgctg
     1741 aaggggtcga cgcagaccac gagccgtccg ccccgcaaca cgaactggtc gatcgcgaag
     1801 agcgtctgat cggagaggtc cttcggatgg accacgatga gggttttgac atcgttgtcg
     1861 atcgtttccg tgtcgggacg aacctcccgg acgacatagt ccttgcgcag ttcgcgaaaa
     1921 gccatccagc cctgatcctg ctgctggcgg cgctgcatca tcatcatggg gttctggggg
     1981 gaaccgagca cgctcaggct gctcagtacg ccgagcaccg gtttttccgg ccaggccgcc
     2041 cgggtgatga ggcgcgtgat atcatattcc agcgtggctt ccgtacgcgg gttgaagccg
     2101 ggtatcacct gttcctgatc cccgctgacc gcgaccagcc cgaaatacac cggttggccg
     2161 aaggggttgg tctgttgcgg ctcgatgccg taacgctgcg cccactcctc ggagtccgag
     2221 tcgggtttcg ggtcataggc ctccagcgtg atgcgtcccc gtcccgcgat ctcatactct
     2281 ttgagcagat cctgcacttg cgtggcatac gttttgaggc ccatgggcat atcggcggaa
     2341 cttgcgctga agtagaattt cagcaccaca tcgttctcca gtttgcccag cacctgccgc
     2401 gaaccctgcg agagggtata gagcttctca gcggtgagat ccacccgcag gcgcaggttc
     2461 gcgatgatga ggttggcggc gccgagaatc accagcaggg cggcgagacc cgcggcaccg
     2521 gtgccgatgc gtgaaaatcc ttttgtttgc ttgtcagaca tagctgtacg ctcccaatac
     2581 gtgatggctg tgttacgatt tcgatgggct tacgaggcct tgcggttgtc ggtgacgaca
     2641 tgtgcggcgg ccagcatgaa gaggatcacg ctgacgtagt agccgatgtc atggaagtcg
     2701 atgaccccgc gccgcatggc atcaaaatgc cgcatcaggc tgcacgacgc gacggcatcg
     2761 accagcccgg cgggtacgcc ccaaccggcg acggcgttga tcacggggtc gaacccgatg
     2821 atcagcataa acaggcagat cgccaaggcg atcacgaagc cgatgacctg gctacgcgaa
     2881 agggccgagg cgaagacccc gatggcgaca caggcccccg aaagcagcag cgacccgagg
     2941 tagccgcaga agatcgcccc catgtcgggg ttacccaacc atgccgtcgt cagcacgatc
     3001 gggaaggtca acgccagcgc gatgccgata aaggcccagg ccgcgagaaa cttggccacg
     3061 agggtctccg ccagcgtcac cggcaaggtg agcagcagct cgatcgtgcc ggtacggcgc
     3121 tcctcggccc agagccccat ggtggccgca ggcaccagca gcagataaac ccagggatgc
     3181 cagaaaaaga agggcatcag gttggcctgg cgccgctcgt aaaacatggc cacgccgaag
     3241 gtcaggaatc cgatcagcac gagaaaggcg accagaaaca cataggccac cggcgagtcg
     3301 aaataggatt tgaactcgcg ttcgaaaatg gacttgatgt ttttaacatt acacatggga
     3361 aacaccctcc gtcatggtca ggttgcggaa caccacgtca aggcgtccct ggggatcttg
     3421 cgcgcgcaac tcagcgggtg tggcattggc gaccacctcg ccgtccgcga tcacaatcac
     3481 ccgcgtgcag accgcgtcca cctcctccag aatgtgggtc gaaacaatga tcgccttgtc
     3541 tgcggccatc tccttgatca tctcgcgcac cacgaatttc tgattcgggt ccagcccgtc
     3601 cgtaggctcg tccatgatca gcaccggggg gtcatgtaaa atcgcctgag cgaaacaggt
     3661 gcgctgccga taccccttcg aaagcgtctc gatggtctgg ttgcggaccg tctccagacg
     3721 gctcttcttg atcaccgact caacctgatc tttcagttcc tgacccgtgt atccccgcac
     3781 cttgccgatg aaagtgagaa aatctgtcac agtcatggtg tgataacttg gcgcattctc
     3841 ggggagatag ccgatacagg ctttggcggc aaccggttcg gtcaagatat catgaccgca
     3901 gatcgttgcg gttcccgctg ttggcgggat gaacccggtt atcatccgca tcgttgtgga
     3961 tttgcccgcg ccgttcggcc cgagaaaacc aagaacttcg cctttttcga cagtgaacga
     4021 tacatccttc accgcctgga tcgggccgaa gttcttttta aggttactta cggtcagcat
     4081 cctgactcct cttcgtcttg ggttgttggt tgaaacagga tcccttgcca gagcagaggg
     4141 atgacaaaaa atcacgtcga aagtagcaga aagtgtgcca ttttttccac ttcatggggt
     4201 acacgctcag tacgggagat tgtgctcctt gattttacgc tggatggtgc ggcgcgagag
     4261 gccgagttgg tcggcggtca gcgtgcggtt gccgttgttg cggcggaggc cctcctccag
     4321 cgccgcgcgc tcgatctgtt cgagcgtcat gtcctgggga aacgttaggc cttgcggtgc
     4381 cgcgccatcg ggatccgcac gcgccttctc gtcgggcaaa gccgcggagt ccgcgtcaag
     4441 ttcaccatcg ggcgagagca gcacggcgga ttccacgacg ttgcggagtt gccgtacatt
     4501 gccgggccag gagaaggctt cgagagccgc gtaaaattcc gggcggacgc aagtgatggt
     4561 cttgttgttc tccacggccg cccgcgcgat gaaacggtcg gtcatcgggc ggatgtcctc
     4621 gcgccgttcg cgcaacggcg gtatgcgcag ggtcacgacg ttgaggcggt agaacaggtc
     4681 ttcgcggaag gtcccttcgc gtaccagggc atcgagatcg cggttggtgg cggagaccag
     4741 ccggacattc acgctgaaca cctctttgcc gcccacgcgc cgcacctcct tctcctccag
     4801 ggcgcgcaac aacttgacct gcacggatgc gggcgcgtcg ccgatctcgt ccagaaagag
     4861 cgtgccgccg tccgcgcgct cgaacgcgcc tttgtacatg ccgatcgcgc ccgtgaaggc
     4921 tcccttctca tggccgaaga gttcggattc gaggaggttt tccgcgaagg cgccgcagtt
     4981 cacggctacg aacggtccgt ccgcacgctt ggagtcgaca tgcagggcgc gggcgaccag
     5041 ctctttgccg gtgccgctct cgccgcagat cagcacggtg gcggaggtgt cggccagcgc
     5101 ctcgatttgc cggatgacct ttttcatgcc gggcgattcg gcgaggatcg cggtggaggc
     5161 gtatttttcg cggaccgacc gggacagctt gtcgacctgc cgacggaggc gcgagatttc
     5221 gcaggcgcgc cgcagtttcg attggatgtc gtccagatcg agtggtttgg tgacgtaatc
     5281 gtaggcgccc tcgcgcatgg cccgcacacc ggactcgacc gaaccgtagg cggtcatgat
     5341 catcacgggg accatgtcgt cgatcttgcg caggcgggcc aaaacttcca ggccgtccat
     5401 gtccggcagg accaaatcgc tgagcaccaa gtcggggatt tggttccgga acgccgccac
     5461 gccggccttg ccgttggcga cgcagaccgt ggcaaacccc gcatcctccg cggcctcggt
     5521 cacggaacgg gcgttatccg gatcgtcttc aatgatcaga aggttgggtt tggtattcat
     5581 aagaggatta cacatcggag gggggtgggg aggacggggg tgccacaggc aaggtgatgc
     5641 gcagatcggc ccctccgccc tcacgcgtaa ggacttgtat gtcgccgccg cagcgtcgca
     5701 ctgcggtccg tgccagaaag agccccaacc cggttccgcc gggacgtccc gagaaaaaca
     5761 tctcgaagac cttttcacgc tcggccggcg gaatgcccgg accgcggtcc agcacatcca
     5821 gcacgatgtg ccgcccgcgc tcctcgatgg ttgccgtgat ttcactgccc tccggggcga
     5881 actgaatcgc gttcatcagg acattcagca gcgcccgtcg cagtgcgaag gggaatgcgg
     5941 agacggctgg catcgtgtcc ggagcgtgga tcgcgacatg gaccccggcc tgttcgaggc
     6001 gcggggcgag ggtgtctacg cactcccgca caacctgctc gatccgcacg gtttccggtt
     6061 gctcgtcggc cggcttggcg aggaaaagga actcctgaaa caccttgtcc atccgcgcca
     6121 tggtgcgggc gatacgctcc gccagatcac gcacacgctc cgcgcggaac ccgtcggcgc
     6181 gggccatttc ccgtcccagc atttgggcgt cgagacgcac ggcggacatc gggttgcgga
     6241 agtcgtgcag gattccgttg gccagaacgc ctgaaaaggc gaggtgttct tcacggcggg
     6301 ctcgctgttc gcgccgccgg tcgcgcgcga caaagaacac cagcaccgct gcacagagcg
     6361 cgaaggagag aaacaccacc gccagcgaaa accagaagag cgaagagacc agcgaacgtg
     6421 cggtccgttc ctctgccccg accgcctcgc ggcggaaggt ggcttcggtg accacccggc
     6481 cgccatcatc cagcgtctgg gtgcgtgtga tgacgaaaac cgactgcggc tcgccgttga
     6541 tttccagggt gccgggagcg atctcggtct cgacccgccg ctctttttcc cgggcacggg
     6601 attccgcgct ctccccttgc gggggatgga gtccgcctgc ctggcgttgg aaaacggtga
     6661 tgccgttgcg cgaaaccgaa acggactgga cgtccttgcg gaccgcgaag gcactgtcca
     6721 caacggttga gaaccgccgc cattccgctg gcgagggatg ggttgaagag caatcgccca
     6781 gatcgcacaa cgcctgcagc agcgcttccc cctcctgcag cacggtggca gtcgccgcca
     6841 gatgcgcctg ccggtcgtgc cggcctttga gcaagaccag cacgcatcct gcggcaaacg
     6901 ccgaggtcaa aagcgcgctt aagaggatga gttccaaacg ccatcctccc cgtgcgcccc
     6961 gattgacggg tctatcgctc tgtctcatgc ggtttttgcc tcagcaggga acgtgccggg
     7021 gtcggggtgc ggcatgtgcc gcgccgcctg accgtttccg gttcctgaaa aaaattgccg
     7081 gattataagg gttcacgcgg aggaagacaa gcggaatgcg acaaattgac gcgtgcatcg
     7141 cgtcagattg tcgcgtgcgg tcaaatcttg gatggtttgg gtccgccacc cggatggatg
     7201 cgccgtgcag gatcgatggg atcatttcaa ccgttcgact ccaaaaggaa cagttgtttt
     7261 tcacacaaag ggtaaaaagg ggaatggggt gtcatgaaaa acctgtgtct tctttgtatg
     7321 agcctaaatt gagttgcggg cttcgtccgc gttaggtgtg tggcatatga attgcttggg
     7381 ttaatacgga gcaaattgag gaatgaccat gcggaatgcg aagttcattg gaagggcatg
     7441 ggcggcgctg gactactttt tgttggttgc cgttttggga ttgttggtca cgcttgcctt
     7501 cttgggacgt tctgtggggg aggccgcggc agacggaaac ggtgagattt caggcggaac
     7561 ggaaccggtt gaacgggttc gaccgttcaa gtcccgcgtg gtcgagggtc tgcgggtttc
     7621 ggcggggcgt caacggctcg acgtgatcgc gcaatccatg gccgcatcgg actgggaccg
     7681 tctgccggcg agcccggcct tggacatgcg ggagaacggg caggcctatg agattctttt
     7741 ttcgctgccc tcgggtgtgg accgcgacaa cgtgcgggtt acttcatccg gaagcctgct
     7801 cacactcgcc ctgaccgatc ccggcaccgg aaaggtgctc atgcggcgca ttcgaatccc
     7861 gtgcgaggcg gaaccaaaca gccgcatcca cagtgttgtc tccaaccggg tgctgcacgt
     7921 tcggatttat ccgtgaccag gcgcgggccg cggggccttc ccgacggcac ctcaacagca
     7981 gcgccgatgc cgttgttttc gcttttttga cactccctca agcaaggtgg tacgatcttc
     8041 ctgctcaagc gaggaggctt tttgtgaaac ccattgtccg gttattcctt acgggacttt
     8101 tcgtcctgtc cggtcgggtg gccgcacaac aggcttcagc ggttgccgcg aacatggaag
     8161 acatgggcaa cccggttgtc aggcggaaca aggtgaagga gcggatgcgt gtcgagc
//
LOCUS       DAOSRB010000125         8151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8331_length_8151_cov_6.084363, whole genome shotgun sequence.
ACCESSION   DAOSRB010000125 DAOSRB010000000
VERSION     DAOSRB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8331_length_8151_cov_6.084363"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..2652)
                     /locus_tag="PLZ74_07620"
     CDS             complement(343..2652)
                     /locus_tag="PLZ74_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98227.1"
                     /translation="MRKTDLSGEWNLYKRGSKKSIPAHVPGCVHADLMAAGEMEDLVR
                     GTRLEAARWVMGEEWVYEKTFVQEDVSAYDRVVLCFGGLNGCAAIRLNDAELGQVTNP
                     FETVEFEVKPLLKAGKNRLRVTFSPPTSTVPGAEEGPNQDFISLSPTVGIGRTVSLLA
                     FTHVRVSEVHIRQDFATAGVVKLHVAVASERYDPAMHLELLVRVCYKGNILHEARDLL
                     NRDRMTLSLTVKNPQLWWPAGLGEQPLYEVTVDVLSGRTCYEHVARRIGLRQVEMEPV
                     RGRAFGRVKVNGQSVFLKGASWVPADLYPARLTRVEYARLVKAVAVANMNCLRVWGGG
                     LYENDAFYDLCDEYGICVWQDLMLGGSRGAPPAKGERASFEREVRSTVMALRHHPCLA
                     LWCGGDGGGKAIDSAYTKIAVDGVRAADPDTPCLPPIPHQPFSLTEPAGYMTMPSFPE
                     PRVIAEYLDEGERNAGHPACAFHMSPSDGARRIYHGFLDHFLVPSSFDHVAWLSQIQQ
                     AVLLRRAYERVRTAKEASPGFLYWHFNDCWPTCSPSAVDYAGRWKALQYLARRFFAPI
                     SLCGVYCRESGTVTLNVFHDGLRPTFKGEMQWRVTQMEGIVVAEGTKKVSVAPLSRDE
                     SVRVKVADILKRCGADNLIMWIYLLDEQGNQVAWNTVLFCEPRALKLQAPRMRAEIRA
                     WDDNSFAVTLTSHHPALWVWISLDGMDARMDDNFFSLEPDKPFRVRVIPATRMKLDAF
                     RQILRIGSLRDTWQDKRLLMQVMAAAKKK"
     gene            3044..5518
                     /locus_tag="PLZ74_07625"
     CDS             3044..5518
                     /locus_tag="PLZ74_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOR98228.1"
                     /translation="MTPELLAPAGGFEAACAAFQYGADAVYLGLPRFSARADAEHLTP
                     DRLRLLKAYAQSFTPAKRVYVTFNTLVTESEWEPALDLLAVLDDLTPDGVIVQDLGVA
                     RLIRDHFPRLALHASTQLATHNLEGALALRELGFTRVVLARELTLDEIRRISQESGMQ
                     TEIFIHGALCYSYSGLCLFSSLATGRSGNRGRCAYCCREPFTAAECASDNAAPHFPFS
                     MRDLCLAPLLDDVTGSGAHALKIEGRMKSPLYVACVTDYYRRKLDRTLSPETERERLQ
                     DLQTIFSRSGTQLYAQDPHAQPETIIDPLTVGHRGARIGEVEAVIRDRDGTRWLRFRS
                     DRALEKHDGLQLEFPGEGRPPGFAVMRLRQAGTRRPALSLAAGERVEVALPEGPFPPI
                     VRGTPVFCSASQAVRRRYGIQSVRESGLTTGRPVDFEVRLDPGGIAVRASTPCEPCGQ
                     VTAETQIALPLSPSRQPEQSPAAVQKAFSRLGDSAWRLGTLTFHDPDGRYAPPSQLNA
                     CRRDVLVRLNGAWDQHAATRREAILRKWRPEPAIPSPSPDAAPLPHWTLKHRADAQPL
                     PTAALSGLSTLILRIGHAPADALKRQLEAWREAVPQTAICPALPVITRACATREMRDA
                     LRVLAREGWERWECADLSGWRMLTEEGLSPYSADWSLYALNRAACAEYARLGFAVQVL
                     SPENGLDNIRALCHIPACAGHRPEAEMPVYLHPPLFLSETAPLTGGRPPPDKGRTRDI
                     RIANRRGKTFTVLVRDRLWVTTADEPYCLADRLPELHAAGVGRFRIDLSWSPETIDAE
                     RTIRNVMAGKLPQSHVLRGNFDRGLA"
     gene            complement(5575..>8151)
                     /locus_tag="PLZ74_07630"
     CDS             complement(5575..>8151)
                     /locus_tag="PLZ74_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOR98229.1"
                     /translation="AGMGELHLDVYVERMRREEAEAFARSFGIPVRGQRPNGGAWELA
                     AIEGGKPIYRMTLNANAAISTGANILRLAPYGADGAGDTVGVWDACSARTTHREFGGR
                     VVSRDGAGVVHDHSTHVVGTICAAGIDSLAKGMAPAVLVDSYDWNDDQGEMTMRGAAY
                     PGEADKIKVSNHSYGMVAGWGYSGNPKFTWYGTGFTASAIEDDFGKYNTYAHEMDKLA
                     FDLPYYLVFWAAGNDRGPSENPVNGDTVALSAGGIPVTYDSSLHPPADHVYRNGYDTL
                     GYSALAKNVVTVGAVTDAVSGGMRSVANANMTIFSSWGPADDGRIKPDLVANGDRLKS
                     TSYGGDSSYSYSSGTSMACPNAAGTAQQLVHLFANLFSNQVMRASTLKALLIHTADDL
                     GNPGPDYVYGWGLLNGKAVADLLLDYRAQAGRRQVIENRVSTDRNAISFEMIWDGASP
                     VRATLCWTDPPGVATGIGDSRQPRLVNNLDLRVIAPDGTVHEPWVMPFVGEWSVASCA
                     YPAITGSNTTDNVEQVLIAAPVVEGTYTVRVTFAGTLTNGQQPFSLIVSGGVPEQQAP
                     RPVLISSSPGSGEGVLPFTFTGDHFLLGATARLRRLGQPDALAMNMQVLGDVAYARVN
                     TVGMSSGWWNLTLANPDGGRATLYNAFVVPELFWSETFETNDLLSRGWSFLQTLGTNQ
                     WELSTVKSVSPSNSAFSLGVDTRSDTSLVSPPIEIPLDQTALQLSFWHDFSFSANDAG
                     VLEFSLDNGDWYDVTDIGSGVVFSQNGYTGTVAGKGAPNSLNPLVGRSAWVGTSGGFV
                     PVTLSLTDTARYAGRQLRVRWRLGTDNSTASEGWYADDVRGLGVSAPPPVPPDGTVFG
                     VR"
BASE COUNT         1650 a         2822 c         2214 g         1465 t
ORIGIN      
        1 tcgatcccga tagcgatccc gaccccgacc ccgatttgga tgaaaccctg aaaccctgaa
       61 accctgaaac cagaaaccct gaaaccagaa acccaagaaa gagtcccgtg gtggaagaat
      121 cgatcccgat agcgatcccg atagcgatcc cgaccccgac cccgatttgg atgaaaccct
      181 gaaaccagaa accctgaaac cctgaaaccc tgaaacccaa gaaagtgtcc cgtggtggaa
      241 gaatcgatcc cgatagcgat cccgaccccg accccgattt ggatgaaacc ctgaaaccag
      301 aaaccctgaa accagaaacc tgaaacccag aagcaatgca cgctatttct ttttggccgc
      361 ggccatcacc tgcatcaaca ggcgtttgtc ctgccaggtg tcgcgcaggg aaccgatgcg
      421 gaggatctgg cggaaggcgt cgagcttcat ccgggtggcc gggataacgc ggacacggaa
      481 tggcttgtcc ggttcaagcg agaaaaagtt gtcatccatc cgcgcgtcca tgccgtcgag
      541 cgaaatccag acccacaggg caggatggtg actggtgagc gtcacggcga agctgttgtc
      601 atcccaggca cggatttcag cccgcatacg cggtgcctgc agtttcagcg cgcgcggctc
      661 gcagaacagc accgtattcc acgccacctg attgccctgc tcatccagca gatagatcca
      721 catgatcagg ttgtccgcgc cacagcgttt caggatgtct gccactttga cgcgaaccga
      781 ttcatcacgg gacaacgggg ccaccgacac ctttttcgtg ccctcggcaa ccacgattcc
      841 ctccatctgg gtcacccgcc actgcatctc acccttgaac gtcggccgga gcccgtcatg
      901 aaacacattc agcgtgaccg tgcccgattc ccggcagtaa accccgcaca gcgagatcgg
      961 cgcgaaaaaa cggcgggcca gatactgcaa cgctttccac cgtcccgcgt aatccacggc
     1021 ggacgggctg cacgtgggcc agcagtcatt gaaatgccaa tagagaaatc cgggtgaagc
     1081 ctccttcgcg gtccgcaccc gctcataggc ccgccgcaac agcaccgcct gctggatctg
     1141 gctgagccaa gcgacatgat cgaaactgga gggaaccaga aaatggtcga ggaacccgtg
     1201 atagatgcgg cgcgcgccat cggaaggcga catgtgaaac gcgcaggccg gatgtccggc
     1261 attccgctcc ccctcatcca gatattccgc gatcacgcgc ggctcgggga acgaaggcat
     1321 cgtcatgtac ccggccggtt ccgtcaggga gaacggctgg tgcgggatcg gcggcagaca
     1381 gggcgtgtcc ggatccgccg cacgcacacc gtcgaccgcg atcttggtgt acgcggaatc
     1441 gatcgctttc ccgccaccgt ccccgccgca ccaaagcgcc agacacggat ggtggcgcaa
     1501 cgccatgacc gtgctccgca cttcccgctc gaacgaggcc cgttcccctt ttgcgggcgg
     1561 cgcgccccgg ctgcccccga gcatcagatc ctgccacacg caaatgccgt actcatcgca
     1621 cagatcataa aaagcatcgt tttcgtaaag ccccccaccc cagacgcgca gacagttcat
     1681 gttcgcgacc gccacggctt tcaccagccg ggcatactcc acccgcgtca agcgcgccgg
     1741 atacaggtcg gccggcaccc aactggcccc cttgaggaaa accgactggc cgttcacttt
     1801 cacacggccg aaggccctgc cgcggaccgg ttccatctcc acctgacgga gcccgatccg
     1861 ccgcgcgacg tgctcgtagc aggtccggcc cgacaaaacg tcaaccgtca cttcgtacag
     1921 cggttgctca cccagaccgg ccggccacca aagctggggg ttcttgaccg tcagggagag
     1981 tgtcatccgg tcccggttaa gcaggtcgcg cgcctcatgc aggatgttgc ccttatagca
     2041 gacccgcacc agcaactcca aatgcatcgc ggggtcatat cgctctgacg ccaccgccac
     2101 atgcagcttg accacgcccg ctgtcgcaaa gtcctgccgg atatgcacct cgctgacccg
     2161 gacatgcgtg aaggccagca gcgaaaccgt gcgcccgatc cccacggtgg gcgacaggga
     2221 gatgaaatcc tgattcggcc cctcctccgc ccccggcacg gttgacgtcg ggggggagaa
     2281 ggttacccgc agacggttct tccccgcctt caacaagggt ttaacctcga attcaaccgt
     2341 ttcaaagggg ttggtgacct gacccagttc ggcatcgttc aatcggatcg ccgcacaccc
     2401 gttcaacccg ccgaaacaca aaaccacccg gtcataggcc gaaacatcct cctgcacaaa
     2461 ggttttttca tatacccact cctcacccat aacccaacgc gccgcctcaa gccgggttcc
     2521 gcgcaccaga tcctccatct cgcccgcagc catcaaatcg gcatgcacac accccggcac
     2581 gtgtgccgga atgctttttt tagagccgcg cttgtagaga ttccactcgc cagagagatc
     2641 agtcttcctc attcgcctca aatccttttc ggtatagata cagaacgttt cataaccgct
     2701 cgtttcgtga cggttcatat tctgtccgaa aaagagctct ttcgtcaact tgatttcctc
     2761 cataatcaga accttcaaag gcatcgggcc cgctccaatt ccgggaaaaa atctaaaaat
     2821 cgcacttgca ttccctcttt ttttctgcta catatttctt tgctgttcga gaacagtttg
     2881 ttgtttcaag gctgtttctc catgtgtact cctatgtatc cccccaagac cggcttgcaa
     2941 aagccggtct tctatttcat attcatacgt cggcatcgcg acggaaagga tggatcatcc
     3001 attccgcacc acgccgttgg ttttgtaaca agaagttcgt gccttgaccc ctgaactcct
     3061 agctcccgca ggcggtttcg aggccgcgtg cgccgccttc caatacggcg cggacgcggt
     3121 gtatctcggc ttgccgcgct tctccgcccg cgccgacgca gagcacctca ccccggaccg
     3181 gctgcgcctg ctgaaggcct atgcgcagag cttcactccc gccaaacggg tgtacgtcac
     3241 cttcaacact ctggtgaccg agtcggagtg ggagccggca ctcgacctgc tggccgtctt
     3301 ggacgacctg acgccggacg gcgtgatcgt gcaggacctg ggcgtggccc ggctgatccg
     3361 cgaccacttc ccccggctgg ccctccatgc cagcacgcaa ttggcgacgc acaatctgga
     3421 gggagcgctc gcactccggg aactcgggtt cacgcgggtg gtgctggcgc gcgaactgac
     3481 ccttgacgaa atccgccgca tttcgcaaga aagcggcatg cagaccgaga ttttcattca
     3541 cggcgcgctt tgttacagct acagcggtct ctgcctcttt tcctctctcg ccaccgggcg
     3601 gagcggcaac cgcggacggt gcgcctattg ctgccgcgaa cccttcaccg cagcggaatg
     3661 cgcgtccgac aacgccgcgc cacatttccc cttctcgatg cgcgacctgt gcctcgcgcc
     3721 gctgcttgac gacgtgacgg gcagcggcgc gcacgcgctg aagatcgaag gccgcatgaa
     3781 gagtccgctc tatgtggcct gcgtcaccga ttactaccgc cgtaagttgg accgcaccct
     3841 ctcccccgag acggaacgcg aacggcttca ggatctccag acgatcttca gccgctccgg
     3901 aacacaactc tacgcccaag acccacacgc gcaacccgaa acgatcattg atccgttgac
     3961 ggtcggccac cgcggcgcac ggatcgggga ggtcgaggct gtgatccgcg accgtgacgg
     4021 cacacggtgg ctgcgtttcc gttccgaccg tgccctagag aaacatgacg gcttgcagct
     4081 cgaattcccc ggggagggcc gtcccccggg ttttgcggtt atgcgcctgc ggcaggccgg
     4141 aacgcggcgt ccggccctct cgctggccgc gggcgaacgc gtcgaagtgg cgctaccgga
     4201 aggcccgttc ccgcccatcg tgcgcggcac accggtgttc tgctccgcct cgcaagccgt
     4261 ccgccgccgc tacggaatcc agagtgtgcg cgagtctggt ctcaccacag ggcgtccggt
     4321 ggatttcgag gtgcgactcg atcccggggg aattgccgtc cgggcctcga ccccctgcga
     4381 accctgcgga caggtcacag ccgaaacgca aatcgccctg cccctttccc cttcgcgcca
     4441 acccgagcaa agtccggccg ccgttcagaa agccttcagc cgcctcggtg actctgcgtg
     4501 gcgtctgggc accctcactt tccacgatcc agacgggcgc tatgcgcccc cctcgcaact
     4561 gaacgcgtgc cggcgcgacg tgctcgtccg cctcaacggg gcgtgggacc aacatgccgc
     4621 aacgcgccga gaggcgatcc tgcggaagtg gcggccggag cccgccattc cgtctccctc
     4681 acctgacgcc gcgccgctcc cgcattggac cctgaaacac cgcgccgacg cgcaacccct
     4741 gccgactgcg gcgctgtcgg gcctgtcaac cttgatcctg cggatcggcc acgcccccgc
     4801 cgatgcgctg aagcgacagt tggaggcgtg gcgcgaagcc gtcccccaga ccgcgatctg
     4861 ccccgcgctg ccggtcatca cgcgcgcgtg cgctacacgg gaaatgcggg acgccctccg
     4921 tgtccttgca cgcgagggat gggagcgctg ggagtgcgca gatctctcgg gctggcgcat
     4981 gttgacagag gaaggactca gcccgtactc cgccgactgg agtttgtacg ccctgaaccg
     5041 ggccgcctgc gcggaatacg cccgtctcgg tttcgcggtg caggtgcttt ccccggagaa
     5101 cggactggac aatatccgtg cgctctgcca catcccggcg tgcgcggggc accgacccga
     5161 agccgaaatg cccgtttatc tgcacccccc tctctttctc tctgaaaccg cccccctgac
     5221 gggcggacgc ccgccccccg ataaagggcg gacgcgggac atccggatcg ccaaccgccg
     5281 cggcaagacc ttcaccgttc tcgtccggga tcgtctatgg gtaaccaccg ccgacgagcc
     5341 ctactgtctg gccgaccggc ttccggaact ccacgccgca ggcgtaggtc gtttccgtat
     5401 cgacctttcc tggtcaccgg aaaccattga tgcggagcga acgatccgaa acgtgatggc
     5461 cgggaagctg ccgcaaagtc atgttctgcg gggtaatttc gaccgcggcc tcgcctgaat
     5521 ccacaccatg aaactcctgt tgcccctcgt tttcgcccgg ccctcggagg ggggtcaacg
     5581 gactccaaac accgtgccgt cgggaggaac cggtggcggc gcggagaccc ccaatccgcg
     5641 cacatcatcc gcataccatc cctcactggc cgtgctgttg tccgtaccca aacgccagcg
     5701 cacgcgcaac tggcggcccg cgtaccgtgc cgtatcggtc agggacaacg taaccgggac
     5761 aaaccctccg cttgtgccca cccaggcaga acgccctacc agcggattca gcgaattcgg
     5821 cgcacccttg cccgccacag tcccggtata cccgttctgg gaaaaaacaa cccccgaacc
     5881 gatatccgtc acgtcatacc aatcgccatt atccagcgaa aactccagca cccccgcgtc
     5941 attggcgcta aacgagaaat catgccagaa gctcagttgc aaagccgttt gatccaacgg
     6001 gatctcgata ggaggggaaa ccagacttgt gtcagatcgt gtgtccacac ccaacgaaaa
     6061 ggcggagttc gacggcgaga cgctcttaac cgtcgagagc tcccactgat tggtccccag
     6121 cgtctgcaga aacgaccagc cgcgggataa aaggtcgttt gtttcgaatg tttcactcca
     6181 gaacagttcc ggcacgacaa aggcattata caacgtcgcc cgtccgccgt ccggattggc
     6241 cagcgtgaga ttccaccatc cgctggacat gcccacggtg ttcacgcgcg cataggccac
     6301 atcccccagg acctgcatgt tcatcgccag ggcatccggt tgccctaacc ggcgcaatcg
     6361 cgccgtagcc cccaacagga aatgatcccc tgtgaaggtg aacggcaaaa ccccctctcc
     6421 cgaacccggc gaagaggaaa tcaaaaccgg tctcggcgcc tgctgttccg gcacgcctcc
     6481 gctcacgatc agagaaaagg gctgttgtcc gtttgtgaga gtgcccgcaa acgtgacacg
     6541 gaccgtatac gtcccctcga caaccggcgc cgcgatcaga acctgctcga cattatccgt
     6601 ggtgttcgaa ccggttatcg cgggatacgc gcaggaggcc acgctccatt cccccacaaa
     6661 cggcatgacc catggctcgt ggacggttcc gtcgggagcg atcacccgca ggtcgagatt
     6721 gttgaccaga cggggctggc gggaatcccc tatcccggtc gccacacccg gcggatcggt
     6781 ccagcaaagc gtcgcgcgga ccggacttgc cccatcccaa atcatttcaa aactgattgc
     6841 attccgatcg gtactcacgc ggttttcgat cacctgccgc ctaccggcct gcgcacggta
     6901 gtcgagcagc agatccgcaa cggccttgcc gttgagcagt ccccaaccgt aaacataatc
     6961 cggaccggga ttccccagat cgtccgctgt gtggatcagc aacgccttga gcgtactcgc
     7021 gcgcatgacc tgattactga agagattcgc gaagaggtgg acaagttgtt gcgccgtgcc
     7081 agccgcattc ggacaggcca tgctcgtccc gctcgagtaa ctgtacgagg aatccccgcc
     7141 atacgaagtt gacttcaaac gatccccgtt ggcaaccaga tccggtttga tgcgcccatc
     7201 gtcagccggt ccccatgagg aaaaaatcgt catgttcgcg ttggcgacac tccgcatgcc
     7261 cccactgacc gcgtcggtga cggcaccgac cgtcacgacg tttttcgcca gcgcactgta
     7321 tccaagcgtg tcatacccgt ttcgataaac atgatctgcg ggcggatgca gggacgaatc
     7381 ataggtgacg gggatgccgc cggcgctgag tgccaccgta tcgccgttca ccgggttttc
     7441 cgaggggccg cgatcattcc cggccgccca gaagaccaga taataaggca ggtcgaaagc
     7501 caacttgtcc atctcgtggg catacgtgtt atatttgcca aaatcgtctt cgatcgcgga
     7561 cgccgtgaat ccggtcccgt accaagtgaa tttcggattc ccgctatacc cccatcccgc
     7621 gaccatccca taactgtgat tcgaaacctt gatcttgtcc gcttcaccgg gataggccgc
     7681 accgcgcatg gtcatctcac cctggtcgtc attccaatca tagctgtcca caagcacagc
     7741 cggagccatt cccttcgcca agctgtcaat ccccgcggca cagatcgtgc cgaccacatg
     7801 cgtcgagtga tcgtgcacga cacccgcacc gtcccgggaa accacccgtc ccccgaattc
     7861 cctgtgggtg gtccgcgctg aacaggcatc ccatacgccg accgtatccc cggctccgtc
     7921 cgcaccgtag ggagcgagac gcagaatatt cgctcccgtg gaaatcgcgg cgttcgcatt
     7981 cagcgtcatg cggtatatcg gcttcccccc ttcgatcgcc gccagttccc atgcaccgcc
     8041 attgggacgc tgcccccgca ccggaatccc gaaggaacgc gcgaacgcct cagcttcttc
     8101 gcgccgcatc cgctcgacat acacgtccag atgcagctcg cccatgcccg c
//
LOCUS       DAOSRB010000126         8132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8354_length_8132_cov_6.428996, whole genome shotgun sequence.
ACCESSION   DAOSRB010000126 DAOSRB010000000
VERSION     DAOSRB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8354_length_8132_cov_6.428996"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PLZ74_07635"
     CDS             complement(<1..1397)
                     /locus_tag="PLZ74_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOR98230.1"
                     /translation="MPRRSYLMSVVALLLATITRCAETPTWRPETRWRGFNLLELFSY
                     RGQPAAFREHDFRLISELGFNFVRLPLDYRFWICDGNWEAFDDSKLAPIDQAIAWGKK
                     YGIHVQLCFHRAPGYTVAQPSEPHNLFSDEHALRVCCHHWSRFARRYQGIPSHAVSFN
                     LFNEPGEVEEAAYAKVAGALVAAIRTEDPDRFIVADGIAWGGRPAQSLFKLGIGQAMR
                     GYKPMSISHYLASWVGTPSAEPIWPPPQAVSPLYGPIKAPLNVPLVMERVPAGTLTLR
                     LGMVSGRVCLRVTADEAVVLKCDLDPKPGTAGWTNVVYKSEWKISQGRCLSVLSVDLP
                     KGAQRLSVSIPQGDWAQLSQVTLTGKSGQAESLWFEQTWGKTNRVFRFMGFGACPAFQ
                     SGAEACDGLTYLRQNVINPWQPAFDAGIFTMVGEFGAFKHTPHPLVLKWMEDNLKLWK
                     EAGLGWALWNFRGTFG"
     gene            complement(1416..3272)
                     /locus_tag="PLZ74_07640"
     CDS             complement(1416..3272)
                     /locus_tag="PLZ74_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOR98231.1"
                     /translation="MKPLMSIQLFAFFAAAVAYGESVKPKIVDALQPITLKQSELSGE
                     IGRRINDLIYTNFMALDVDGFFVAPFRSRPFTNGYHYVGVGKVIEAGSLFAAYTGDPK
                     IADRTRHLIDGMIGTRDADGYIGVFKPEPDDRQNHWNWALHEQEYLLLGLTRNALLTH
                     DPKMLQDAQALADYMIRTFSKDPHPEQICTAGLPEAFLVLYKATGDTRYFDFAANIRH
                     GNGTAEIKCASLRDWRQTFETRPAHVYVMLARCYAQAELYRYDGVASLMDMSKYMRHE
                     LLRPGGGLNVVGSASDGEHFSYTQNGAGAIGESCVTAYLLRWLDSLMRLEGDLRYGDL
                     MERTVYNALFAAQDPAGRKLRYFTPFTGVRAYYGQDGFCCPGNYRRIVAELPQKVYYR
                     CSDGALSVNLFTASKVTVPVSPDATVTLAQKTDYPNTGHVVFRVTPSKETVFTLRLRI
                     PRWCRTATVTVNNESPLSVLPGAKAFELRRVWKTGDTVTLDMPMAWRFVKGHQLQEGK
                     AVLMRGPIVYCLGTAQNSEVLEKYPNFSGVVIDPTCIGEIENDTSIRPDGKKVKAQAR
                     VEAPGAGRKGSPHVTLTFTEFIDPTGIATYVHLLDLNKAEEDELVSDCFAKP"
     gene            3572..4873
                     /locus_tag="PLZ74_07645"
     CDS             3572..4873
                     /locus_tag="PLZ74_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR98232.1"
                     /translation="MKKNIAIKLGLLSVLTVMAQEGGLPDVAPKAGWDTLAPVKKSEN
                     DWPWWRGPNFDNIAPPGQKPPTAWGAGSNILWQVKIPGVGHSSPCVVGNRIYITSGIK
                     TQGQTTLWLFCLDRNTGKTVWQAELYIGASAKVHADNSPASSTPACDGERVFVAYQTD
                     SSVVMVAVDVEGKIRWRQTVAPYNTIQGFSASPNIYKSAVIIPVEGPKGSYLTAFHRA
                     TGEVVWRTKLRAVNEGYAPAVVTVIAGREQLLQLGGTYTRGIDPENGTLLWECAGPAK
                     KVCVATAAFDRDTVYATGGYPNRILLAIRADGKGEVTKSHIRWSSDAKVGYVPSPILY
                     EGLLYAVADQGLLRCYDTVDGRVLWEQDFKVPFYSSPTLADGKLYLFDRKGKGYILPT
                     GRMAGAIVTNELPSGVFATPVFLENRIYLRTLGDFCCIGEK"
     gene            4931..7858
                     /locus_tag="PLZ74_07650"
     CDS             4931..7858
                     /locus_tag="PLZ74_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOR98233.1"
                     /translation="MKKVLISLFSVTALSFAACLDVRADEKDRRPVIFWASEPVRPGE
                     TAMVIGENLGDHPAIEIVRLPDGRAKEPSQENVVWPRGGTPVDVLQAAEQSAKFIVPA
                     DFKPGLLRLRLTAGGAAVEHIMNRPQIWWVQGECGPAVTPGGWIRFFGKNLGWGIKDG
                     IRKPVIRIEGPCVVSLAFETDGHAARAVLPKDMPSGSYRLFAHNGAGGRAGWSAPVEL
                     TVTNKEVWPQNVFNVTNFGALADGVKDDTVAIQNALSAAESQGGGIVYLPLGRYAVSD
                     TLNIPRRTVLRGERSDWVALAWSDMRNPPWALVQGTNSFGLEDLTIYAHHHHHVIAAD
                     LGDKPDAGNVFLRRVRVRASSYRGHLTPDEVDARFRETMKWSTGGGDTVRMGGSSIEI
                     TECDFYGSGRTLFLSRVRGGLVAGNRFFNGRWGWYCISGSDGLIFENNEITGADLMST
                     GGGLNCLDGSRFSQNVYYADNRLQRMHGWDREAMTSDAGGEAYSGKAVTAHGTTLVLD
                     GKPNWRGQDWRGAGVFVLAGKGAGQYRRVAGQGEQSVIVDRPWDVVPGTNSDICVTMF
                     QGHYILSGNEFSDCGAVQFYGTAVENIVVRNTGTRMSGFRGIGLWYHGYQPNWFCEFR
                     DNAIKEGNYYHWNRASDALLEITGYRRAPYTGPLNVGSVVRHNRLDSQAHIRVGAATR
                     DAIVEGNHVANSAQGIFVSVHATNIWLHANTFEKVREEIMDDLKQRRIAQERLSRHPF
                     FNRPEPVAVWDFEKTERMNVADVSGNGFRARLNGGATTVSNGVGGRAGRFDGTGWLCV
                     NEVIVFNAPDMTVSLWIKPETLRGRRGLVGKRFSGCEAPFILSQNGARLSFEATDTEH
                     QWSFNFVSEPVLKEGQWTHVAAVVRRGKGVALFANGKKVAEKQNASDRIVNHEPLILG
                     REAWGGEPASTSTPGYFIGLMDEVRIWTRALSPDEIEKEASRRPAGNGFPRSSGSQPI
                     TKR"
BASE COUNT         1734 a         2251 c         2490 g         1657 t
ORIGIN      
        1 ccgaacgtcc ctctgaaatt ccacagcgcc cagcccagtc ctgcttcctt ccagagtttc
       61 aggttgtcct ccatccactt cagcactagc ggatggggcg tatgcttgaa ggcgccgaac
      121 tcgccgacca tcgtgaaaat ccctgcatca aaggcgggtt gccaagggtt tatcacgttc
      181 tgacggagat aggtaagccc gtcgcaagcc tccgcgcctg attggaaggc ggggcatgcg
      241 ccgaatccca taaaacggaa cacgcggttg gtcttgcccc acgtttgctc aaaccacagc
      301 gattccgcct gacccgactt tccggtcaag gtgacttggg agagttgcgc ccaatcgcct
      361 tggggaatgc tgacggagag ccgctgcgcc cccttcggaa gatccacgct caacacggaa
      421 agacatcgcc cctgcgaaat tttccattcg cttttgtaca ccacatttgt ccagcctgcg
      481 gtgcccggtt tcgggtctag gtcacatttc agcaccacgg cctcatctgc ggtcacgcgc
      541 aggcaaactc tgcccgacac catcccgagc cgcagggtca gggttcctgc agggacgcgc
      601 tccattacca gcggcacatt cagcggagct ttgatcggcc cataaagtgg actgacggcc
      661 tgcggcggcg gccatatcgg ctccgcagat ggtgtgccga cccaactcgc cagatagtgc
      721 gagatcgaca tgggcttgta gccgcgcatc gcctgcccga tcccgagttt gaaaagcgac
      781 tgggcggggc gtccgcccca tgcgatgccg tccgccacaa tgaagcggtc cggatcctcc
      841 gtccggatgg cggcgaccag cgcacccgcc accttcgcat aggccgcttc ctcaacctcg
      901 ccgggctcat tgaaaaggtt gaagctcact gcatgcgagg ggatcccttg atatcgccgc
      961 gcgaaccggc tccagtgatg gcaacagacg cgcagcgcgt gttcatcgga aaacaagttg
     1021 tgcggttccg aaggctgggc cacggtgtaa cccggcgcgc gatggaagca gagctgcaca
     1081 tggatgccgt acttcttgcc ccaggcgatc gcctgatcga tgggcgcgag tttagagtcg
     1141 tcgaacgctt cccagtttcc gtcgcaaatc cagaagcggt aatccagggg cagacgaacg
     1201 aaattgaacc ccaactcgct gatcaggcga aagtcatgtt cccgaaatgc ggccggctgt
     1261 ccccggtacg agaacagttc gagaaggttg aagcctcgcc aacgggtttc cggacgccaa
     1321 gttggggttt ccgcgcaccg cgttatggtt gcgagcaaca gtgcgactac ggacataagg
     1381 tacgatctgc ggggcatccc ggctcctttt aacaatcacg gtttggcaaa acagtccgac
     1441 acgagttcgt cttcctcggc tttgttgagg tcaagcagat gcacataggt ggcgatgccc
     1501 gtcggatcga tgaactccgt aaaggtcaac gtcacgtgcg gcgaaccttt tcgccccgct
     1561 ccaggtgctt caacccttgc ctgagccttg acctttttcc cgtcagggcg aatcgacgtg
     1621 tcgttttcta tctctccgat acaggttgga tcaatcacca ctccagaaaa attcgggtat
     1681 ttttccagta cctcgctatt ttgtgccgtg cctaggcaat acacaattgg accacgcatc
     1741 agcaccgctt tcccctcttg taactggtgg cctttgacaa accgccacgc catcggcata
     1801 tccagcgtca ccgtgtcgcc ggttttccac acacggcgca gttcgaaagc cttggcaccc
     1861 ggcaagacgg aaagcggaga ctcgttgttg actgtgacgg ttgcggtgcg gcaccagcga
     1921 ggaattcgca gacgcagggt gaacacggtt tcttttgacg gcgtcacgcg gaacaccaca
     1981 tgcccggtat tcggataatc cgttttctgt gcaagcgtca cggtcgcatc gggagagacc
     2041 ggcaccgtca ctttcgaggc cgtgaacagg ttgaccgaca atgcgccgtc cgagcaccgg
     2101 taataaactt tctgaggcaa ctccgccacg atgcggcgat aattacccgg acaacaaaaa
     2161 ccatcttgcc cataataggc tcggacgccg gtaaacggcg tgaagtaccg gagtttcctt
     2221 cccgcagggt cttgcgccgc aaaaagcgcg ttgtaaaccg tacgctccat cagatcgccg
     2281 taccggagat ctccctccag gcgcatcagg ctgtccagcc agcgcagcaa atacgctgtc
     2341 acgcacgatt cgccgatagc ccccgcaccg ttctgcgtat aggaaaagtg ttcgccgtca
     2401 gaagccgagc cgaccacatt gagcccgccg ccggggcgca gcaattcgtg gcgcatatac
     2461 ttcgacatat ccatgagtga cgccaccccg tcgtaacggt agagttccgc ctgcgcgtag
     2521 caccgcgcaa gcatcacgta gacatgcgcg gggcgggtct cgaaagtctg gcgccaatcc
     2581 cgcagtgacg cgcatttaat ctcggccgta ccattaccat gacggatatt ggcggcaaaa
     2641 tcgaaatagc gcgtgtcgcc tgtggcctta tataaaacga gaaaggcttc gggaagtccg
     2701 gcggtgcaga tttgctcggg atgcggatct ttcgaaaagg tacggatcat ataatccgcg
     2761 agcgcttgcg cgtcctgcaa catcttggga tcgtgcgtga gaagcgcgtt gcgggttaat
     2821 ccgagcaaca gatactcctg ctcatgcaat gcccagttcc agtggttctg acgatcatcg
     2881 ggctcgggct tgaacacgcc gatgtatccg tcggcatcgc gcgttccgat catgccgtca
     2941 atcaggtgac gcgtccgatc cgcgattttc gggtcgcccg tataggcggc gaacaggctt
     3001 cccgcctcga tgacttttcc aacgccgaca tagtgatagc cgtttgtgaa ggggcgcgac
     3061 cggaacggcg caacgaaaaa gccgtccaca tcaagcgcca tgaagttcgt atagatcaga
     3121 tcgttgatcc gcctgccgat ctcgcctgaa agctccgact gtttcaatgt gatcggctgg
     3181 agagcgtcga cgattttcgg ctttacactt tctccgtagg ccaccgcggc ggcaaaaaac
     3241 gcaaagagtt ggattgacat caacggtttc atcagttggc tccagtcgtt ttatcacact
     3301 ttcgaaggtg tgatagggga cagatttctt ttacatgatc actataaaag attgatgttc
     3361 cgggcggaag tcaaattcgc gcaggacgaa tatcaataaa acataaaatc tggggatcgt
     3421 cctggtcagg ttgcggttga ctgatacgat cctttttttt atgtttagtt acccctgacg
     3481 cttcggatgc cgggcgaagg cttcgatcgt tggcttcgct tggaatcagg gattctgtcc
     3541 ggaaagcttc ggggcgagga gtaatacgag cgtgaaaaaa aacatcgcaa tcaagttggg
     3601 tttacttagt gtgctgacgg tgatggcgca ggaggggggg ctgcccgatg tcgcgccgaa
     3661 ggcgggttgg gacacgcttg ctcccgtgaa aaagtctgaa aacgattggc cgtggtggcg
     3721 ggggccgaac ttcgacaaca tcgcgccccc cgggcagaag ccgcctacgg catggggcgc
     3781 cggttcgaac atcctgtggc aggtaaagat accgggagtc gggcactcgt ccccttgcgt
     3841 ggtcggcaac cggatttaca tcacttcagg gatcaagacg caggggcaga caacgctctg
     3901 gctcttttgc ctcgaccgga ataccggcaa aacggtgtgg caggccgagc tatacatagg
     3961 ggcttcagca aaagttcatg ccgacaattc gccagcctcg tctacgccgg cctgcgacgg
     4021 cgagcgggtc tttgtggcgt accaaaccga ttcgtcggtc gttatggtgg ccgtggatgt
     4081 ggaggggaaa atccgatggc gccagacagt tgccccgtac aacacaattc aaggtttttc
     4141 cgcgtcgccg aacatttaca agtcagcagt gatcattccg gtcgagggcc cgaaagggtc
     4201 ttatctgaca gcgtttcacc gggcaaccgg cgaagtggtt tggcgcacga agctgcgtgc
     4261 cgtcaacgaa ggctacgcgc cggccgtggt tacggtgatt gccgggcgcg aacagttgct
     4321 gcaactgggc ggcacataca cgcgcggcat cgatcccgag aacgggacac tgctctggga
     4381 gtgtgcagga ccggcgaaaa aagtctgcgt cgcgacagcg gcctttgacc gggatacggt
     4441 ctacgcgacc ggcggatatc cgaaccggat cctgctcgcc atccgcgcgg acggcaaagg
     4501 agaggtcacg aaaagccata tccgctggtc cagtgacgcg aaggtcggat acgtcccttc
     4561 cccgattctg tacgaggggt tgctgtatgc ggtcgcggac caaggtctgt tgcggtgtta
     4621 cgatacggtt gacgggcgcg tgctctggga acaggacttc aaagtgccgt tctattcttc
     4681 gccgacgctg gcagacggca agctctacct gtttgaccgc aaaggcaagg gatatatcct
     4741 tccgacaggg cggatggcgg gggcgatcgt gaccaacgaa ctgccgtcgg gggtgttcgc
     4801 cacgccggtc tttctggaaa accgtatcta tctgagaacg ctgggcgatt tctgttgcat
     4861 cggagagaaa tagatgagta tccggcggca caagtgcccg gatttctgat cacccgtgag
     4921 aaggagcagg atgaagaagg ttcttatttc actgttttcg gtgacggccc tcagttttgc
     4981 ggcctgtctt gatgtacggg ctgatgaaaa agaccggagg ccggtgattt tttgggctag
     5041 tgagccggtc cgtccgggtg agacggcgat ggtgatcggc gaaaaccttg gcgaccaccc
     5101 agcgatcgag attgtgcggc taccggacgg ccgggccaaa gagccgtcgc aagagaacgt
     5161 cgtttggccc agaggcggca ccccggtgga tgtgcttcag gcggccgaac agtctgcaaa
     5221 attcatcgtg ccggcggatt tcaagccggg gttgctcagg cttcgtctga ccgcaggggg
     5281 cgctgcggtt gagcatatca tgaaccgccc gcagatctgg tgggtgcagg gggagtgcgg
     5341 tcctgccgtg accccgggcg ggtggatccg gtttttcggg aagaaccttg gctggggaat
     5401 aaaagacgga atcaggaaac cggttatcag gatcgaagga ccgtgtgtcg tttcccttgc
     5461 gttcgaaacg gacggtcatg cggcgcgcgc cgtgttaccc aaggacatgc cgtcaggatc
     5521 gtaccgcctg tttgcccaca atggcgctgg cggccgtgcg ggatggagcg caccagtgga
     5581 gttgacggtc acaaacaaag aggtctggcc gcagaacgtg ttcaatgtga caaatttcgg
     5641 cgctctggcg gacggcgtga aggacgacac ggttgcgatt caaaacgcac tatcggcagc
     5701 cgagtctcag ggcggaggca ttgtctattt gccgctgggc cgttatgcgg tttccgacac
     5761 cttgaacatc ccgcggcgca cggtgctccg gggcgagcgg tcggactggg tcgcgctggc
     5821 atggtcggat atgcggaatc cgccttgggc actggtacag ggaacgaatt cattcggact
     5881 ggaggacctg acgatctacg cgcaccacca ccatcatgtg atcgccgcag acctaggcga
     5941 caaacccgac gcggggaatg tgtttctgcg gcgtgtgcgt gtccgggcca gttcgtatcg
     6001 cggacatttg acgcctgacg aggtggacgc gcgttttcgc gaaacgatga aatggtccac
     6061 aggaggcggc gatacggtga ggatgggcgg gagcagcatt gagatcacgg agtgtgattt
     6121 ctacggttcc gggcgcacgt tgtttctgag ccgcgtacgt ggcggacttg ttgccggtaa
     6181 ccgttttttc aacggtcgct ggggctggta ctgcatctcg ggaagcgacg gactgatttt
     6241 tgagaacaac gagatcaccg gtgcggatct gatgtccacc ggcggcggct tgaattgtct
     6301 cgacggctca cgcttctcgc aaaatgtgta ttatgcggat aaccgtttgc aacggatgca
     6361 cggctgggac cgcgaggcga tgacatcgga cgccggtggc gaggcatatt ccggaaaggc
     6421 cgtaacggcg catggaacga cgctcgttct tgacggcaag ccgaactggc gcgggcagga
     6481 ctggcgcggt gcaggggtgt ttgtgcttgc gggcaaaggc gcggggcaat accgccgtgt
     6541 ggcagggcag ggtgagcagt ccgtgatcgt ggaccgtccg tgggatgtgg tgccgggcac
     6601 gaattcggat atttgcgtca cgatgttcca aggacattat attctcagcg ggaatgaatt
     6661 ctcagactgt ggggctgtgc agttttatgg aacggcggtg gaaaatatcg tcgtgcggaa
     6721 cacgggcaca cgcatgtccg gattccgcgg catagggtta tggtatcacg gctatcaacc
     6781 gaactggttc tgcgagttcc gcgacaacgc gatcaaagag ggcaactatt accactggaa
     6841 tagggcgtcc gatgcgcttc tcgaaatcac agggtacaga cgcgcccctt acaccgggcc
     6901 gctgaacgtg gggtcggtcg tgcggcacaa ccggctcgac agccaggcgc acatccgtgt
     6961 cggcgccgcg acgcgcgacg ccatcgtgga aggaaaccat gtggcgaatt ccgcgcaggg
     7021 catttttgtc agcgtacacg caacgaatat ttggcttcac gccaacacgt ttgagaaggt
     7081 ccgggaagag ataatggatg atctgaagca acgccgtatc gctcaggaac gtcttagccg
     7141 tcacccgttt ttcaaccgcc cggagccggt tgccgtctgg gactttgaga agaccgaacg
     7201 aatgaacgtc gccgatgttt ccggcaacgg tttccgcgcc agactcaacg gcggcgcgac
     7261 aacggtgtcg aacggggtgg ggggccgtgc gggaaggttc gacggcacag gttggctgtg
     7321 cgtgaatgag gtgatcgttt tcaatgcacc ggacatgacg gtttcgctct ggatcaagcc
     7381 agaaaccctg cggggcaggc gcgggttggt cggcaagcgg ttttccggct gcgaggcgcc
     7441 gttcatcctg tctcagaacg gtgccaggct cagctttgag gccaccgaca ccgaacacca
     7501 atggtcgttc aacttcgtat cggagcccgt attgaaggag gggcaatgga cgcatgtcgc
     7561 tgcggtcgtg cggcgcggca agggcgttgc gctctttgcg aatggaaaaa aagtcgccga
     7621 aaaacagaat gcgtccgacc ggatcgtcaa ccatgaaccc ctgatccttg ggcgggaagc
     7681 ctggggtggc gagcccgcat caaccagcac gccgggatat ttcatcggcc tgatggacga
     7741 ggtgagaatc tggacacggg cactctctcc tgatgagatt gaaaaagagg cctcgcgccg
     7801 accggccgga aacggattcc cgcgatcttc gggctcacaa ccgattacaa aacggtaatg
     7861 ttccggcaag tgtccggcaa tgctgagtga aaccatgccg caaaaacctt catgcccttt
     7921 gtaccttctg tgagaaaaac cgagccatgc atgaaaccag aaaccttgaa accctgaaac
     7981 ccaagaaagt gtcccgtggt gaagcaatcg atcccgatag cgatagcgat cccgatcccg
     8041 atttggatga aaccctgaaa ccatgaaacc caagagagtt gaaggttcaa ctgctctttt
     8101 taggttcatg gtagggacgc ctccccgagg cg
//
LOCUS       DAOSRB010000127         8066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8429_length_8066_cov_6.738984, whole genome shotgun sequence.
ACCESSION   DAOSRB010000127 DAOSRB010000000
VERSION     DAOSRB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8429_length_8066_cov_6.738984"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLZ74_07655"
     CDS             complement(<1..1038)
                     /locus_tag="PLZ74_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98234.1"
                     /translation="MVRETTGIAAATIILCGLTSLHAQPRDTPLPGRGAALFVQGFFE
                     QDTFTEGARLFPDRTYTVAEAPAWLRGLTFLRANIDGNLTVTAKQAGVLTVITADPAD
                     PCATHSQCARLEKLGFVWIKAPATFQLFGKAAYDISRVYQKQVAQDETFRFPKWTVFA
                     GFSAVTGPPPPFDLQPGRGERLYNGIELPTNWPPRTVNTADWAPMAVPYLDVPPELIH
                     IDVGRQLFVDDFLIATSTLQRVFGMPEKYSGNPVLRPETELELNGIRNAAAVPKGGGL
                     WWDPHEHLFKLWYEAGWIHTICYATSTNGLDWVRPELDVVPETNQVLPPDLTPDSWTV
                     VPDWEATDPLQR"
     gene            complement(1294..3891)
                     /locus_tag="PLZ74_07660"
     CDS             complement(1294..3891)
                     /locus_tag="PLZ74_07660"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOR98235.1"
                     /translation="MKKRIPFAAVLIFAGAAMQIRGAELAPRLIINEDNDHYFKQKSE
                     LMTEAALRAYIDTFADTQVTHFFMCPQGQRTSYRSRVHEAIWDPVNGVTPTNIWCVNC
                     KLLFDKGIDPYHVWIEQCRKRGISPWITMRMNDVHFVNIPDYFRNTTFWRERRDLWRN
                     PDTTTNNWMECAFDYSKEEAYAYHMAVVRELFERYDFDGFETDWLRFPYHLTPGKAFA
                     QRDVLTRFMREVRALADLWAKKRGHPIRISARVASDPDVAAHLGTDAVAWARDGLIEL
                     LVASCFFSTADFDIPIERWHARLGDLKDRVPVIPGTDNGFSPYPGSPRQSLDIHLYRG
                     WAEACRYRGANAFYLFNLVYFSFDRPPFRPIIEKGFPAPILAQEPRRHTITYRDALPS
                     EGGFESGAQLPQTTETPCRFVIQTGQKPQYGKVSVITGLAAHPQVSNTVLTVSLNGTR
                     ATNRTVEPQVRRYGKLTAYAVRHTFPLSALRDGSNTVDVASAPQPRQRIEWVEIEITP
                     ATPLEHVGPPLPRHAVTNRAFTGISSLAVAPNGRLWVTWYAGHTPNEDNNNYVVLSTS
                     GDHGRTWKEVLIADPDGSGPRRTFDPEVWLAPDGKLRWFFTDRKNGLPATDLLWMIVL
                     DDPGSESTAWHPPAHIADGVMMCKPIVLSTGEWALPVCTWYTENSSKMVVSTDSGKTW
                     SVRGGASMPKEDRLFDEHMFVERKDRSIWCLTRTKSGIREAVSTDRGATWSPLEPSPI
                     QHPSARFFITRLLSGNLLLVKHGPIGKKTGRSHLTAFVSRDDGKTWEGGLMLDERNGV
                     SYPDGQQTADGTIYITHDFDRTGKRHILFSTFREEDALAGKPVSGAVRLRQLVSEGSG
                     GKERPQATK"
     gene            complement(3932..5941)
                     /locus_tag="PLZ74_07665"
     CDS             complement(3932..5941)
                     /locus_tag="PLZ74_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98236.1"
                     /translation="MTKRILFALAFTVQSAWAIVNWVAKTDPAQNDFSNLDNWPAAIA
                     TDKAIQFYTLNPAAPELWMSEDATFARLVLGGTNIVFNLGSGRTLSVGQEGETKLNAA
                     KTFVRLTSGTINQTANRLVLGQDKNSSNCVFIADGPTTRYLNPQRGIHIGAASNAVSC
                     GMGVYNGAVVDGNFYIGNHAFSSGNWVEAAGTGTVVLATNNHFYVGDTAASSWNRLSL
                     SESAALQMMGANFCIGNNSGSDSNQAKILSGSSVTGQSETIVGNKGSENTLEAGNATL
                     KMSSLFTIGYYSGSNSVRLTNTFVQSNGIYLGRDPSSSGENTLEVIGGTVTNTGGGIR
                     VGIYGNRNTVVISNAYVCPAGNLSAGEYGSENSLSLYGDGTETRVAGLIFGGNAEAHK
                     NTLRLSGCVITNTGGFGNNGAENRIEIANGSKIWSGGHVGGTAAGVSNVLNLTGGSFF
                     SATTAFNFGNVSSFNTMIVDNSAFIASNVTFCLGYTEGTANSNTLAVINNGYLYAYRL
                     RIGDRGSYNTLIISNSTVHAGSNNCTIPYQNGISTNNQMVFIGTNCLLKSEADIYIRS
                     GTTLTFVIPRGGYAEPPIQTLADTAKNINIDPTTRIVLDLTEFARGGGGEQVLARTDN
                     NFSISETTLSLLNAQIAPQQCALEIVGKELILHTPHTGGTVISLW"
     gene            complement(6004..7671)
                     /locus_tag="PLZ74_07670"
     CDS             complement(6004..7671)
                     /locus_tag="PLZ74_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98237.1"
                     /translation="MTFFRSVLTVSVFCLIAFGALAQPAPRPASDAPMPVIINMAADG
                     VVNFITGHFRKEGTNRAVAEAAIDKEVDQYVGTGVSHLFWNVNYQRVGYDSKAWPSYW
                     DVPDPETQVTEWPRGYYLLHKLGINDVFARLIPRSRAKGISPWISLRMNDHHYFNDFS
                     RVSPLLFNHPELRVNRTGLFNYTHPEVRDHYMKLIVEVLTRYDIDGLELDWMRTPNNF
                     PEQELDAGRELLTAFVREARRHAQAATQRRGHPVHLAVRVPATPEFAVAEGLDAVRWA
                     REGLVDMIVVSDFWGGCGDFPIERWRAQIGPDGAACKLIACTQCTYLCVPKGFQFVNN
                     IAAQRGFTASMADRGAEGIYLFNHFHAVTGIQRLRTPEGKSFEEGTLADLFRAAADRK
                     SAPAGRRIHPLAIHDGLPEKTDYRPALPAPLAVNNPSASFRLHIGPRPAATAACRLFI
                     GLYATNGVEAAQFDARVNGTACQPLPDLPRPTKDEFLKPKPDNWMTPPRRNLSELAGR
                     VLVFDVPTASLTRGYNAIGLTLTGGLPQSVTWLELVIDPEAGKPVAP"
     gene            complement(7906..8061)
                     /locus_tag="PLZ74_07675"
     CDS             complement(7906..8061)
                     /locus_tag="PLZ74_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOR98238.1"
                     /translation="MNISVVDEQQILEASKNPEAYPDLIVRVTGFTAYFANLSPDFRK
                     LVLERIG"
BASE COUNT         1577 a         2250 c         2552 g         1687 t
ORIGIN      
        1 gcgctgcagc ggatcggtcg cctcccaatc gggcaccacg gtccaggagt cgggggtgag
       61 gtccggcggc agtacttggt tggtttccgg caccacgtcc agttcgggac gaacccaatc
      121 caacccgttg gtgctggtgg cgtagcagat ggtgtggatc cagccggctt cgtaccagag
      181 tttgaaaaga tgctcgtggg gatcccacca gagcccgccg cccttcggca cggccgctgc
      241 gttccggatg ccgttcaact cgagttcggt ctccggacgc agtactgggt tgccgctata
      301 cttctcaggc atgccgaaga cgcgctgcaa cgtgctggtt gcgatcagaa aatcatccac
      361 gaacaactgt cgccccacat cgatatggat caactccggc ggcacgtcca aatacggcac
      421 ggccatcggt gcccagtctg cggtgttcac ggtgcgcggc ggccagttgg tcggaagttc
      481 gatgccgttg taaagccgct cgccgcgccc aggctgaagg tcgaacggcg gcggtggtcc
      541 ggtcaccgca gagaaaccgg cgaacaccgt ccacttgggg aaacggaacg tctcgtcctg
      601 cgcgacctgt ttctgataga cgcgactgat gtcgtaagcc gctttgccga aaagctgaaa
      661 ggtcgccggt gctttgatcc agacaaaccc cagcttctcc agacgcgcgc attgcgaatg
      721 cgtggcgcac ggatcggcgg gatcagccgt gatcacggtc agcacaccgg cctgcttggc
      781 cgtcacggta agattgccat caatgtttgc gcgcagaaag gtcaacccgc gcaaccaggc
      841 gggtgcttca gccaccgtgt aagtgcggtc cggaaagagc cgcgcaccct cggtgaacgt
      901 gtcctgctca aaaaatccct gcacgaagag cgcggcaccg cgccccggca aaggtgtgtc
      961 gcgtggctgt gcgtgaagcg atgtcagccc gcacaggatg atcgttgcag ccgcgattcc
     1021 cgttgtttct ctcaccattc tttctctccc caaaccatga aacacatgaa acttgaaaat
     1081 tgcatacgtc tcttaaccat gaaacacatg aaacttcatg aaatcaagga ctcctttcat
     1141 cagtcgttac tcactttctc ccgataccga taccgatttc gatgaaacca aactaacgca
     1201 ctaacgcact aacgcactaa cgaactaatg aactaacgca ctaatgaact aaccacaccc
     1261 cgcttttcat gaagtttcat gtgtttcatg gtatcattta gtggcttggg ggcgctcttt
     1321 tccccccgat ccctcgctca caagctgacg cagacgcacc gcgccggaaa ccggcttgcc
     1381 ggccagcgca tcctcctcgc ggaaggtgga gaagagaatg tggcgtttac cggtgcggtc
     1441 gaagtcatgg gtgatgtaaa tcgtgccatc tgcggtctgt tgaccgtccg gataggagac
     1501 cccgttgcgc tcatcaagca tcaggccgcc ttcccacgtc ttgccgtcgt ctctggaaac
     1561 aaaggcggtc agatgcgagc ggccggtctt ctttccgatc ggaccatgct tcaccagcaa
     1621 caggttgccg gaaagcagcc gcgtgatgaa gaagcgcgcc gaagggtgct ggatcggcga
     1681 cggctccagc ggcgaccacg tcgcaccgcg gtcggtcgag accgcctcgc ggataccgga
     1741 cttggtacgc gtcaggcacc agatcgagcg atctttgcgt tccacaaaca tgtgttcgtc
     1801 aaagaggcgg tcctccttgg gcatactcgc accgccgcgc acggaccacg tcttgccgct
     1861 gtcggtggag accaccatct tcgaactgtt ctccgtatac caggtgcaga ccggtaacgc
     1921 ccactcgcct gtagagagca caatcggctt gcacatcatg acgccgtcag cgatatgcgc
     1981 gggcggatgc caggccgtag actcggaacc gggatcatcg agcacgatca tccagagcaa
     2041 atcggttgcc ggtaatccgt tcttgcggtc cgtgaagaac cagcgcagtt ttccgtccgg
     2101 tgcgagccaa acttcgggat cgaaggtgcg gcgcggaccg gatccatcgg ggtcggcaat
     2161 caacacttcc ttccaggtcc ggccatggtc gccgctggta ctgagcacaa cgtagttgtt
     2221 gttgtcttcg ttgggagtgt gtcccgcgta ccaagtgacc cacagacggc cgttgggtgc
     2281 gaccgccaga ctggagatac cggtgaaggc ccggttggtc accgcgtgtc tgggcagcgg
     2341 cgggcccaca tgctccagcg gcgtggcggg tgtgatctcg atttcaaccc attcgatccg
     2401 ttgccggggc tgtggggcgg acgcaacgtc caccgtgttg gagccgtctc gcagggcgga
     2461 gagcgggaag gtgtgacgca ccgcatacgc cgtgagcttg ccataacgcc gcacctgggg
     2521 ttcgaccgtc cgattggtgg cgcgcgttcc attgagcgaa accgtcagca cggtgttcga
     2581 gacttgcgga tgggcggcca gtccggtgat cacggagact tttccgtatt ggggtttctg
     2641 gccggtctga atcacaaaac gacagggcgt ctccgtggtt tgcggcagtt gcgcgccaga
     2701 ctcaaacccg ccttccgagg gtagcgcgtc gcggtaggtg atcgtgtgcc gacgaggctc
     2761 ttgtgccagg attggggccg gaaacccctt ttcgatgatc ggacggaaag gggggcggtc
     2821 aaaggaaaag tacaccagat tgaaaagata gaaagcgttc gcgccacggt aacggcaggc
     2881 ttccgcccaa ccgcgataga gatggatatc caatgactga cgcggtgacc cggggtaggg
     2941 ggagaatccg ttgtcagtgc cgggaatgac cggaacgcga tccttgaggt cgccgaggcg
     3001 cgcgtgccag cgttcgatcg ggatgtcgaa gtccgcagtg gagaaaaagc aggaggcgac
     3061 gagcagttcg atcaatccgt cgcgcgccca cgccaccgcg tcggtgccga gatgggccgc
     3121 cacatccgga tccgaggcca cgcgcgctga aatgcggatc gggtgtccgc gttttttcgc
     3181 ccagaggtct gccaaggcgc gcacctcacg catgaagcgg gtcagcacat cgcgttgcgc
     3241 gaacgccttg ccgggcgtca ggtgataggg gaagcgcagc cagtcggttt caaagccgtc
     3301 gaaatcatag cgttcgaaca gttcccgcac gacagccatg tgataggcgt aggcctcctc
     3361 cttcgagtag tcgaacgcac actccatcca gttgttcgtg gtggtgtccg ggttgcgcca
     3421 gaggtcgcgg cgctcgcgcc agaaagtcgt gttgcggaag tagtccggga tgttcacaaa
     3481 gtgcacatcg ttcatacgca tggtgatcca gggtgaaatc ccgcgcttgc ggcactgttc
     3541 gatccagaca tggtaagggt cgatcccttt gtcgaaaagg agtttacagt tgacgcacca
     3601 gatgttggtc ggcgtgacac cgttgaccgg gtcccagatc gcctcgtgca cgcgggagcg
     3661 gtaactggtg cgctgacctt gggggcacat gaagaaatgc gtcacctgtg tgtcagcgaa
     3721 ggtgtcgata taggcgcgta gcgccgcctc ggtcatcaat tcagacttct gtttgaagta
     3781 gtggtcgttg tcctcgttga taatcaggcg gggcgccaac tcggcgcccc tgatctgcat
     3841 ggcggcgccc gcaaaaatga ggacagccgc aaagggtatt cgttttttca taaaggttct
     3901 cgtttccgtt cttcggccgg gacgttggcc ctcaccatag cgaaatgacc gtgccgcccg
     3961 tatggggtgt gtgcaggatc agttccttgc cgacgatctc cagtgcgcac tgttgcggtg
     4021 cgatttgcgc attgagcaac gacagggtgg tttcactgat actgaagttg ttgtccgtac
     4081 gcgccagaac ttgttccccg ccaccgccgc gcgcgaactc ggtgaggtcg agaacgatcc
     4141 gggtcgtggg atcgatgttg atgttttttg ccgtatcggc aagcgtttga atcgggggct
     4201 ccgcgtatcc gccgcgcggg atcacgaacg taagcgtggt tccgctgcgg atgtaaatgt
     4261 ccgcttcgga ttttaaaagg cagttggtgc cgatgaacac catctggttg ttggtggaga
     4321 ttccgttttg gtaagggatc gtgcagttgt tactcccggc gtgaaccgtg ctgttgctga
     4381 tgatcagggt attgtatgat cctctgtctc cgatgcggag gcgataggca tacagatatc
     4441 cgttgttgat gacggcaagc gtgttactgt tggcggtgcc ttcggtgtac cccagacaaa
     4501 aggtgacgtt actcgcgatg aacgcgctgt tgtccacgat catggtgttg aaggaggaga
     4561 cattgccgaa attgaaagcg gtggtcgcag agaaaaacga accgcccgtc aggttcagca
     4621 cattcgagac gcctgccgct gtgccaccga cgtggccgcc ggaccagatt ttcgaaccgt
     4681 tcgcgatctc gatccggttt tccgcgccgt tgttgccgaa ccctcccgtg ttggtgatca
     4741 cgcatccgga tagtctcagg gtgtttttgt gcgcttccgc gtttccgccg aaaatcaaac
     4801 cggcgacacg ggtctcggtc ccgtcgccat acagcgacaa gctgttctcg gaaccgtatt
     4861 cgccggcaga aagatttccg gccggacaca cgtacgcatt gctgatgacg accgtgttcc
     4921 ggttgccata tatgccgacc cggatgccgc cgccagtgtt cgtgaccgtg ccgccgatga
     4981 cctccagcgt attctcgccg ctgctcgacg gatcgcggcc cagatagatt ccgttggatt
     5041 gcacgaacgt gttcgtgagc cggacagaat tggatccgct ataataaccg atcgtgaaca
     5101 gcgaggacat tttgagcgtg gcgttcccgg cctccagcgt gttttcggaa cccttgttgc
     5161 cgacgatcgt ctcggattgg cctgtcacgg acgagccgga caggatcttc gcctgattgg
     5221 aatcagagcc gctgttgttc cctatacaga aattagcgcc catcatttgg agtgcggcgg
     5281 attcggaaag ggagagccgg ttccaactgc tggccgctgt gtcgccgacg tagaaatggt
     5341 tgtttgtcgc caggacaacc gttccggtgc cggcagcctc aacccagttg cctgaagaga
     5401 acgcatggtt gccgatgtag aaattgccgt ccacaacagc gccgttgtaa acgcccatcc
     5461 cgcagcttac ggcgttcgat gcggccccga tgtgaattcc gcgttgcgga tttaggtatc
     5521 gggtggtcgg gccgtcagcg ataaacacgc agttggaaga gtttttatct tgtccgagga
     5581 cgagtcggtt ggcggtttgg ttgatcgttc cgctggtcag ccggacgaat gttttggcgg
     5641 cgttgagttt ggtctcgccc tcctgaccca ccgaaagcgt gcggccggat cccagattga
     5701 agacgatgtt cgtgccgccc agaaccaacc gggcgaaggt ggcgtcctcg ctcatccaca
     5761 gttccggcgc ggccggattc agcgtgtaga attgaatcgc cttgtccgtg gcaatggctg
     5821 cgggccagtt atccaggttt gagaaatcgt tttgtgcggg gtcggttttc gcgacccagt
     5881 tcacgatcgc ccaggcgctt tgcacggtga aggcaagggc gaacagaatg cgctttgtca
     5941 tgacccactc ctttcattat ttgcagtatt gtagcacgtt cacattttga cgaacacctc
     6001 gaatcagggt gcgacgggtt ttcccgcttc agggtcgatg acgagttcca gccaggtgac
     6061 gctttggggg aggccgccgg tcagggtgag cccgatggcg ttgtatccac gcgtcaggga
     6121 tgcggtcggc acgtcgaaaa ccaagacacg tccggccagt tcgctgaggt tgcggcgggg
     6181 gggcgtcatc cagttgtcgg gtttgggttt tagaaattcg tctttcgtgg gccgggggag
     6241 gtcggggagt ggttggcatg cggttccgtt gacgcgcgcg tcgaactgtg cggcttcgac
     6301 accgttggtg gcgtacaacc cgatgaagag gcggcaggcc gcggttgcgg caggacgtgg
     6361 accgatgtgc agccggaagg atgcggacgg gttgtttacg gcgagcgggg caggcaacgc
     6421 gggacggtaa tcggtttttt cgggcaggcc atcgtggatt gcaagaggat ggatacggcg
     6481 gccggcgggg gcgctcttgc ggtcggccgc ggcgcgaaag agatcggcca gcgtgccttc
     6541 ctcaaaagat ttgccttccg gggtgcgcag acgctggatg ccggtgacgg catggaaatg
     6601 gttaaagaga tagatgcctt ccgcaccgcg gtcagccatc gatgcggtaa acccgcgttg
     6661 cgctgcgata ttgttgacaa actggaaccc cttaggcaca cacaggtagg tgcattgggt
     6721 gcaggcgatc agcttgcagg cagcgccgtc gggaccgatc tgcgcgcgcc agcgttcaat
     6781 cggaaagtcg ccgcaaccgc cccagaagtc gctgaccacg atcatgtcca caagaccctc
     6841 ccgggcccaa cggaccgcgt ccagaccctc ggcgacagcg aactccggcg tggccggaac
     6901 gcgcaccgcg aggtggaccg ggtgtccccg gcgttgcgtt gcggcctgcg catggcggcg
     6961 cgcctcgcgg acgaatgcgg tgagcagttc acgccccgcg tccagctcct gctccgggaa
     7021 gttgttgggg gtccgcatcc agtccagttc caacccatcg atgtcgtaac gggtcagcac
     7081 ctcgacgatc agcttcatgt agtgatcgcg cacctcggga tgggtatagt tgaagagccc
     7141 ggtgcggttg acgcgcaact cgggatggtt gaagagcagg gggctgacgc gggagaaatc
     7201 gttgaaatag tggtgatcat tcatgcgcag cgagatccag ggcgagatgc ccttggcgcg
     7261 ggaacgggga atcaggcgcg cgaaaacgtc gttgatgccc aacttgtgaa gcaggtagta
     7321 cccgcggggc cattcggtga cttgggtttc cgggtccgga acgtcccagt agctcggcca
     7381 ggctttgctg tcatacccga cacgctggta gttgacattc cagaaaaggt gggagacccc
     7441 agttcccaca tactggtcca cctctttgtc gatcgccgcc tcggccacgg cgcggttggt
     7501 tccctcttta cgaaagtggc cggttatgaa gttgaccacc ccgtctgccg ccatgttgat
     7561 gatcacgggc atgggggcgt cggatgcggg gcggggggcg ggttgcgcca gggcaccgaa
     7621 ggcgatgaga cagaagaccg aaacagtcag aacggaacga aaaaaagtca tcctctcctc
     7681 caagacgacg cccggctctt cccggacggg cgggcgggtt aaaaacacaa gttttttaaa
     7741 gttgtatacc agtttaaccg ttcggttaga tggcgtcaag agggtgagcg gagatttttc
     7801 gatttcccgg cgacggggaa agggacatgc tcaaatctgg cgggtttttg tttttcggga
     7861 gagccgtatg aggagttcat ctttggcatg ctgttcctca aacggctatc cgattcgttc
     7921 gagcacgagt ttgcggaagt cgggcgagag gttggcgaag taggcggtga agccggtcac
     7981 gcgcacaatc agatcgggat aggcctcggg gtttttgctc gcttccagaa tctgttgctc
     8041 gtcaacgacg ctgatgttca tgagcg
//
LOCUS       DAOSRB010000128         8012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8502_length_8012_cov_6.242554, whole genome shotgun sequence.
ACCESSION   DAOSRB010000128 DAOSRB010000000
VERSION     DAOSRB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8502_length_8012_cov_6.242554"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLZ74_07680"
     CDS             <1..413
                     /locus_tag="PLZ74_07680"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOR98239.1"
                     /translation="LRHRRDRALIPVLAGAGRLGSALLGYPGFQNQGFKIVAAFDANP
                     RKRGRIAGIPVFPADQLTNKSRALGVSLGILTVPADQAQVAADAMMAGGIRGIWNFTP
                     VQIAVPADVIVKHEDLAAGLAVLSHRLRQGGFIA"
     gene            410..1561
                     /locus_tag="PLZ74_07685"
     CDS             410..1561
                     /locus_tag="PLZ74_07685"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOR98240.1"
                     /translation="MIVGMDVLAARRFDALHGRRVGLLSHQAALTRNGTTSAQCLRAA
                     LGNRLAALYGPEHGFFGQAGAGVHTTSRRHPDWGIPVHSLYGERRKPTPAMLSGLDLM
                     VCDLQDLGVRCYTYLATLRNMLEACRDAGLSVLVTDRPIPLPRAVDGPMPDADALGFV
                     TPCMVPLVYGMTPAESALWLRHTLFPDLRLDVVPMEGWTREDAAWDGQRPAFLPPSPG
                     IRTWESAMTYAATVFCEALSGIDCGRNTNLAFRVLGAPWLRAGQTCARLKSLRLAGVA
                     FTPYRYTAAGGTYDGRELDGILLTVTDPARFAPATVSLALLQTLAEQYGPRRVWHHKG
                     VRPTWFDTLYGTATTRRDLRGGRPFREIAAEWRTAQRAFQTARAAVLLY"
     gene            complement(1563..2495)
                     /locus_tag="PLZ74_07690"
     CDS             complement(1563..2495)
                     /locus_tag="PLZ74_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOR98241.1"
                     /translation="MVTIYHWEDGGLREVASPTGACWINLADPSTEEIARIQELTQVP
                     REFLTDPLDKDERPRLEIEDGATLLIVHVPYHEPHENLIPFHTLPLGIVLTQSHVITI
                     CNRLTPVTSTFLDQIRRVCPPEDQFRFTFQLLWHSAILYLRYLRDIRQRADMVEQDLH
                     ESISNEALMRLLNIEKSLVYFTTSLKANDILIGRIEHTRQLDIPDKDLDVLEDVAVEY
                     RQALEMATIHSNILTGTLDTFASIISNNLNNVMKYLTSITIVLMLPTLVASIYGMNVK
                     LPLDQYEYAFPILMGFSLLLSIVVVIVLARRRFF"
     gene            complement(2570..2737)
                     /locus_tag="PLZ74_07695"
     CDS             complement(2570..2737)
                     /locus_tag="PLZ74_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98242.1"
                     /translation="MAGFSYEIVACSGSLTGTFAATPNLPGAWVVRYDTTPGAGKVTL
                     VHDIGTILLFR"
     gene            complement(2755..7845)
                     /locus_tag="PLZ74_07700"
     CDS             complement(2755..7845)
                     /locus_tag="PLZ74_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOR98243.1"
                     /translation="MANVKGFTRKIWNSVPEFGRKIMAFVLMAGSLSAADGVWIAPGD
                     GAWGDTANWEGGAYASGAGFTAFFTNGVPAWIDQNITGLTLGGLYAAGADTLFTNSTV
                     TLNNNGNPATVTVDGPDVTATVALTLDPVAGTAITKAGSGTLRLDRYLKVPAGLTIEG
                     GILDLRFPGSSDGLNVGSGTITVKGGAILRCGAHNQINNSAVIDIQAGGTFDLAGMSD
                     NIGAVTGSGVLTNHSSTIQFWLDGTTRLFSGDCYGGGTFSFKQPGTFVVGGSNTLQNV
                     TFDGRFPGLNLSLAPGIGRFRIGNLHLTNDFALADTEGGPVALELGWKNDSFTLANVF
                     TGVGGLTKTGTGTMTVTNTQTYAGPTVVAEGKLTVTDETLANRLANTSQIQLGTGTVL
                     ELNTTESETNNTPVYGAGTIIKAKESTLALGALNTTNTAVTVSAGTLEVAGGTFSGGG
                     FTQNAGARLVFNGGSSTGATLTVAPGYPVEIRGGTHLFDSALGGTGRYLQTDGTTFFK
                     NTLGNNNTNDIFVTVSGGTMNIGYIQHCQGLGLLACGTAVVNIADNRTRIASDGKTHS
                     LVVTNDAQVTTDGLYLLSHGNTTSTGTVALAGGTLTMKGGFTGVGSQAGDLTYVSLDG
                     GLLRFIGSSSSLAGMYHLYQIREGGARIETATVDSTTTFYQGFTNATGGTIDGGLIKS
                     GPGTLRFLTDNRYTGASILNAGTLTSWGTGDTPFGCGPVTINGGTLEIAPSGSGANLA
                     LSLAHGDTANTVTVGPGVSMLRAARGNHVSLSVTLGAADAAADSVLTRDTHGVLAIWP
                     GAGTSTLGDTEKVIVNGGVATVNGVVPTVFGIHNDDRRSCDFLAYDAADGFKLAAYTA
                     GLGGGDTSIANVTANASTDSVHVHALRVHNGAVLTVNSGQTLTVGDGVNPAGVILNNS
                     SSVTAGLNGGTLDFGTSEGVIMYNLQRANQYGPTLGSVITGSNGLTLVGGGQAGGLQL
                     TAAANTYTGPTRIMAGRVSLANSRGFSDGDVYIYGNEAWGGQFRVTFTGNVTNTLHLA
                     GVGAGTSQQLGAVVFERDSEISGPIELMRDTRVSVSVLAGAIGTLSGPITGPGGLEIG
                     CPDAVIAGTVRLTGANTYGGGTRVSRGTLEIAAQDSLAPGQITVNNGVLAFALNGDVT
                     ITNAIRGVGRVVQKGSGTLTLTDAESFAGTFEIQAGALVVEGADTAMQTAFINGLLDL
                     DGQTVTTGILEGGGVVSNSTVTDAVLTVGTDDASSRFWGSILDGEGAVSLVKKGNGTL
                     TLSGLNAYGGSTTIEAGTLRLQGTQTELPTNGLAYWLDASRADLLTLDGSNVTMWADA
                     SGAGVHFTQEESVLQPLLVTNAASGLPAVRFGGRTNCLIAAKSVLSHSVFVVNEVFGY
                     RDNGGLWGGYGKDIGIRQSSLTSWIHPGNGNDFTCNGRMFVNGIEDRVFSSGVPHVLA
                     ALAEKPQNWVAAIGDYRPYSGSLFRSYRGDINEVAVYSTALGMSDMHSVSEYLRYKWL
                     GLESVFPTNVLPETTVLTLAAGGMLDLNGLNQAVGSLEGAGEVGNRSGRESTFTVGND
                     NRSTFFAGLISGSNALVKVGSGTLALGGANTYLGDTRVNGGTLKLDGGSLASGGTVTV
                     DAGATFDVNGQTQTLAGIGGSGTITGCDKLTVTETIAPGGVGEVGTLTLDGAPVFDSC
                     SLLIDTRKNGQADNLSVAGDLISRRSRW"
BASE COUNT         1510 a         2606 c         2345 g         1551 t
ORIGIN      
        1 ggctgcggca ccggcgcgac cgcgcgctga ttccggtgct ggccggcgcg ggacgtctag
       61 gctccgcttt gctgggatac cccgggtttc agaaccaagg attcaagatc gtggccgcct
      121 ttgacgcgaa tccgcggaaa cgcggccgga ttgcggggat acccgtcttt cccgccgatc
      181 agctcaccaa caaatcccgc gcactgggcg tgtcgctcgg gattctcacg gtgccggccg
      241 accaagcgca ggttgcggcg gatgccatga tggccggcgg gattcggggc atctggaatt
      301 tcacgcccgt gcagatcgcg gtgcctgcgg atgtgatcgt caaacacgaa gatctggccg
      361 ctggactggc ggtgctctcc caccgattgc ggcaaggagg tttcatcgca tgattgtggg
      421 catggatgtg ctggccgcgc gacgcttcga cgccctgcac ggtagacgcg tggggctgtt
      481 gagccatcag gccgcactga cgcgtaacgg cacgacatcg gcgcagtgtc tacgcgccgc
      541 actgggcaac cggctggcgg ccctctacgg tccggagcac ggtttcttcg gtcaggcggg
      601 cgcgggcgta cacacgactt cgcgtcggca tcccgactgg gggatcccgg ttcattccct
      661 ctatggggaa cggcgaaaac caacgcccgc gatgctttcg gggttggatc tcatggtctg
      721 cgatctgcaa gatctcggcg tgcggtgtta cacctatctc gccaccctcc gcaacatgct
      781 ggaggcgtgt cgagacgcgg gtctgtctgt gctggtcacc gaccgtccta tcccgttgcc
      841 ccgagcggtc gatggcccca tgccggatgc cgacgcgctc gggtttgtca cgccctgcat
      901 ggttccgctg gtttacggca tgacgccggc ggagagtgct ctctggctgc gccacaccct
      961 gtttcccgat ctgcgcctcg atgtggttcc gatggagggc tggacccgtg aggatgcggc
     1021 ctgggacggt cagcgtcccg ccttcctgcc gccctccccc ggtatccgga cttgggaaag
     1081 cgccatgacc tatgcggcca cggtcttctg cgaggcgcta tccgggatcg attgcggccg
     1141 caacaccaat ctggcgttcc gcgttctggg tgcgccctgg ttgcgcgcgg gacagacctg
     1201 cgcgcggctg aagtccctgc gtctggcagg cgtcgccttt accccctacc gatacacggc
     1261 ggccgggggc acatacgacg gccgagaact cgacggcatc ctgctgacgg tcaccgatcc
     1321 cgcacgtttc gctccggcca ctgtttcgct agcattgctg cagactctgg cggaacagta
     1381 cggtccgcgt cgcgtctggc accacaaagg cgtgcgcccg acttggttcg acacgctgta
     1441 cggaacggca acgacgcgtc gggacctgcg cggggggcgg ccgttccgcg aaatcgctgc
     1501 ggaatggcgc accgcgcaac gcgcgttcca gaccgcgcgc gccgcagtgt tgctgtattg
     1561 actcagaaga aacggcggcg ggcgagcaca atcaccacga cgatcgagag cagcagcgaa
     1621 aagcccatga ggatggggaa agcgtattca tactggtcca gaggcagctt cacattcatg
     1681 ccgtagatgc tggccaccag cgtgggcagc ataagcacga tcgtgatgga ggtcagatac
     1741 ttcatcacgt tgttgagatt gtttgaaata atcgaagcga acgtgtcgag cgtgccggtg
     1801 aggatgttgc tgtggattgt ggccatctcc agtgcctgac ggtattcgac cgccacatcc
     1861 tccagcacat cgagatcttt gtcggggatg tccaactggc gggtatgttc gatccggccg
     1921 atcaagatgt cgttcgcttt cagggaggtg gtaaagtaaa ccagactctt ctcaatgttc
     1981 agcagacgca tcagcgcctc gttggagatg gattcgtgca ggtcctgctc gaccatgtcg
     2041 gcgcgctgcc gaatgtcgcg cagataccgc agatagagga tggccgaatg ccagagaagc
     2101 tggaacgtaa aacggaactg gtcctcgggc gggcagacac gccggatctg gtccagaaag
     2161 gtactggtaa cgggcgtcag ccggttgcag atcgtgatca catgggactg ggtgaggaca
     2221 ataccgagcg gcagcgtatg aaacgggatc aggttctcgt gcggctcatg atacgggaca
     2281 tgcacaatca ggagcgtggc cccgtcctcg atttccagac gcggtcgctc gtccttgtcc
     2341 agcggatccg tgagaaactc gcgtggaacc tgggtcagct cctgaatccg cgcgatctct
     2401 tctgtggaag gatcggccag attgatccag caggctccgg tcggtgaggc aacctcgcgg
     2461 aggccgccat cctcccaatg ataaattgtg accataacca ccacctctcc gcatcagcgg
     2521 gcatgtttgt actcttatcg cccacctgat gcaagcggtt tcttgatggt taccggaaca
     2581 gcagaattgt ccctatatcg tgcacaagcg tgaccttgcc cgcacccggt gtggtgtcgt
     2641 agcgaaccac ccacgcgccg ggcaggttag gcgtggcggc gaaggtgccg gtcaggcttc
     2701 ccgaacaggc gacgatctca tacgagaaac ccgccatttg cgtgacatca ccgatcacca
     2761 gcgtgagcgc cgagagatca aatcccccgc aacggaaagg ttgtccgcct gaccgttctt
     2821 gcgcgtgtcg atgagcaggg agcaactgtc gaatacgggt gccccgtcga gcgtcagcgt
     2881 gcccacctcg cccacaccgc ccggcgcgat cgtttccgtc acggtcagct tgtcacagcc
     2941 ggtgatggtt ccgctgccgc cgatcccggc cagcgtctgc gtctgaccgt tcacatcaaa
     3001 ggtcgcgccc gcgtcgaccg tgaccgtccc gccggaagcg aggctcccgc cgtccagttt
     3061 cagggtcccg ccgttcacgc gcgtatcgcc cagataagtg ttcgcgccac ccagcgccaa
     3121 tgtgccgctg ccgactttga ccagcgcgtt ggagcccgaa atcaaaccgg cgaagaacgt
     3181 gctgcggttg tcgttaccaa cggtaaacgt tgactcgcgg ccactacggt tgcccacctc
     3241 acccgcgccc tccagcgagc cgaccgcctg attcaatccg ttcaagtcca gcatgccgcc
     3301 tgcggcaagg gtcaagaccg tggtttcggg caacacattg gtggggaaga cgctttcaag
     3361 cccgagccac ttgtatcgca agtactcgga gaccgaatgc atgtccgaca tgccgagcgc
     3421 cgtgctgtag accgccactt cgttgatatc gccccgatac gaacggaaaa gcgatccgct
     3481 gtagggccgg taatctccga tcgcggcgac ccagttctgc ggcttttcgg ctaaagcggc
     3541 cagcacatgc ggcacgccgg atgagaacac cctgtcttct ataccgttga cgaacatgcg
     3601 tccgttgcac gtaaagtcat tgccgttgcc cggatggatc cacgaagtca ggctggactg
     3661 ccggatcccg atatcctttc catagccacc ccaaagcccc ccgttgtccc ggtatccgaa
     3721 cacctcgttg accacgaaaa ccgaatgtga caacacactc ttggcagcga tcaagcagtt
     3781 ggtccggccc ccaaagcgga ctgcgggcaa accactcgcc gcgttcgtca ccaacagcgg
     3841 ctgtagcacc gactcttcct gcgtgaaatg gacgccagcc ccggaagcgt ccgcccacat
     3901 ggtgacattt gatccatcga gcgtcagcag gtcagcgcgt gacgcgtcaa gccagtacgc
     3961 gagcccgttg gtcggcagtt cggtctgcgt accctgcaag cggagggttc ccgcctcgat
     4021 ggtcgtggaa ccgccatacg cgttcaaacc gctcaacgtc agcgtaccat tccccttctt
     4081 cacaagtgag acagcacctt caccatccaa gatcgatccc cagaaccggc tgctcgcatc
     4141 atccgttcca accgtcagca cggcgtcggt caccgtggag ttgctcacca ctcccccacc
     4201 ctccaatatg ccggtcgtca cggtctggcc atcgaggtcg agcaacccgt tgataaaggc
     4261 cgtctgcatc gccgtatccg caccctccac aaccagcgcg cccgcctgaa tctcgaaggt
     4321 tcctgcaaag gattccgcgt cggtgagggt cagggtgcct gatcccttct gcaccacacg
     4381 accgacaccg cggatcgcat tggtgatcgt aacgtcgccg ttaagggcaa acgccagcac
     4441 cccattgttg accgttatct gtccgggcgc caaggaatct tgcgcggcga tctccagcgt
     4501 accgcggctg acacgggtcc ctcccccata ggtgttggca ccggtcaacc ggacggttcc
     4561 cgcaatcacc gcatcaggac agccgatctc caagccgccg ggaccggtga tcggaccgct
     4621 gagcgtcccg atcgcgccag ccagaaccga cacgctcacc cgcgtgtccc gcatcagctc
     4681 gatcggcccc gagatttcac tgtcgcgttc gaatacgacc gcacccaact gctgggatgt
     4741 tccggcgccc acacccgcga gatgcagggt gttggtgacg tttccggtga acgtgacgcg
     4801 gaactgaccg ccccacgcct cgttgccgta gatgtacacg tccccgtcgc tgaagccgcg
     4861 gctgttcgcg agcgacacgc ggcccgccat gatgcgcgtt ggcccggtgt aggtgttggc
     4921 ggcagcggta agctgcaagc ccccggcctg tccgccgccc accagtgtca gaccgttcga
     4981 acccgtaatc acactcccga gcgtggggcc gtactgattc gcccgctgca agttgtacat
     5041 gatgacgcct tccgatgtcc cgaaatcaag cgtgccgccg ttcagcccgg cggtgacgct
     5101 gctggagtta ttgaggatca ccccggcggg attcacgccg tcgccgacag tcagcgtctg
     5161 accgctgttg acggtcagta ccgcgccgtt gtgcacacgc agcgcatgga catggacact
     5221 gtccgtcgag gcgttggccg tcacattggc gatcgaggtg tcgccgccac cgagcccggc
     5281 cgtgtacgcc gccagcttga aaccgtccgc agcgtcatag gcgaggaaat cgcacgaccg
     5341 gcggtcgtca ttatggatac cgaacaccgt cggcaccaca ccgttgacgg tcgccacacc
     5401 gccgttaacg atgaccttct ccgtgtcgcc cagcgtggac gtgccggctc ccggccagat
     5461 cgccagtacg ccgtgcgtgt cgcgcgtcag cacgctgtcc gccgccgcgt cggcagcgcc
     5521 gagcgtcacg cttagactga catggttgcc gcgcgcggca cgcagcatcg acacgccggg
     5581 accgaccgtc acggtattcg ccgtatcacc gtgggccagg gacagggcca gattcgcacc
     5641 gcttccggat ggagcgatct ccagtgtgcc gccgttgatc gtcaccggac cgcacccgaa
     5701 cggcgtgtcg cccgttcccc acgaagtcag ggtacccgcg ttaaggatcg aggctccggt
     5761 gtacctgtta tccgtaagga atcggagcgt cccggggccg ctcttgatca ggccaccgtc
     5821 aatcgtgcca ccggttgcat tggtgaagcc ctgatagaaa gtcgtcgtgc tatccaccgt
     5881 cgctgtctcg atacgcgcac cgccctcccg aatctggtaa aggtggtaca tgcccgccag
     5941 agaggacgag gaaccgatga accgcagcag accaccgtcc aaactcacat aagtcaaatc
     6001 acccgcctgc gatcccacac ccgtaaagcc ccccttcatc gtaagggtgc cccctgccaa
     6061 agcaacggtt cctgtactgg tcgtgtttcc gtgtgacagc agatacaatc cgtcggtagt
     6121 gacctgcgcg tcgttcgtca ccaccagcga gtgcgtcttg ccgtcactgg cgatacgcgt
     6181 gcggttatca gcaatgttca caaccgctgt gccacatgcc aacaggccaa gtccctgaca
     6241 gtgttggatg tagccgatgt tcatggtgcc gccgctaacc gtgacgaaaa tgtcgttggt
     6301 gttattattt ccaagcgtat ttttaaagaa ggtggtgccg tcggtctgga gataacgtcc
     6361 cgtgccgccc aacgcgctgt caaagaggtg agtcccgcct cggatctcga ccggataacc
     6421 cggtgcgacc gtcagggtcg cgcccgtact gctgccgccg ttgaacacca gccgggcacc
     6481 cgcgttctgc gtgaacccgc ctccagagaa cgtcccgccc gcgacttcga gcgtgcccgc
     6541 cgaaaccgtg accgccgtgt tggtggtgtt cagcgcgccg agggccagcg tcgattcctt
     6601 tgccttgatg atcgtgcccg ccccgtaaac cggagtgtta ttcgtctcgc tttccgttgt
     6661 gttgagttcg agaaccgttc cggttccaag ctgaatttgc gaggtgttgg ccaagcggtt
     6721 tgcaagtgtt tcatccgtga ccgtcagctt accctccgcc accaccgtgg gcccggcata
     6781 cgtctgcgtg ttcgtaaccg tcatcgtgcc ggttccggtt ttcgtcaggc cgcccacccc
     6841 ggtaaacacg ttggccagcg tgaacgagtc gtttttccat cccaactcca gcgcaacagg
     6901 accaccctcg gtgtccgcaa gggcaaaatc gttggtgaga tgcagattgc cgatgcggaa
     6961 cctgccgatc cccggcgcga gcgaaagatt cagccccggg aaacgcccgt cgaaagtcac
     7021 attctggagc gtgttggaac ccccgaccac aaaggtgccg ggctgcttaa acgagaacgt
     7081 tcccccaccg taacagtctc cggaaaacag gcgtgtggtc ccgtccagcc agaactggat
     7141 ggtgctggag tggttggtga gcacaccgct gcccgtcacg gcaccgatgt tgtcgctcat
     7201 acccgccaga tcaaacgtac cgccagcttg aatgtcgatg accgcggaat tattgatctg
     7261 gttatgggct ccgcaccgca gtatggcacc ccccttcacg gtgatcgtgc cgctacccac
     7321 attcagtccg tcgctgctcc ccgggaaccg gaggtccaat atcccgcctt cgatcgtcag
     7381 ccccgccggc actttcaaat atcgatccag ccgcaacgtg cctgaccccg ctttggttat
     7441 ggcggtaccg gcaacgggat cgagggtcag cgccaccgtg gcggtgacgt caggtccatc
     7501 gaccgtcacg gttgcgggat tcccgttgtt gttgagggtc acggtgctgt tggtaaacaa
     7561 ggtgtccgcg ccagcggcat acagcccgcc aagcgtcagc cccgtaatgt tctggtcgat
     7621 ccacgcaggc accccgttgg tgaaaaacgc cgtaaagccg gcacccgagg cataggcacc
     7681 gccttcccag ttggccgtgt cgccccacgc tccgtccccg ggcgcaatcc acacgccgtc
     7741 cgcagccgaa agcgagcccg ccatcagaac aaacgccatg atcttccgtc cgaattccgg
     7801 gactgaattc cagattttgc gggtgaaccc ctttacgtta gccataccct gcctccttgc
     7861 ctgaagaaca aaccctgttc aatcaaaaaa tcacggtcaa gaatatctga aaacggaaag
     7921 tctgtcattc gttcttttca ttgcacttca gtccgctctt tcaacttaaa aagagcagtt
     7981 gaaccttcaa ctctctttgg tttcatggtt tc
//
LOCUS       DAOSRB010000129         7989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8524_length_7989_cov_6.157298, whole genome shotgun sequence.
ACCESSION   DAOSRB010000129 DAOSRB010000000
VERSION     DAOSRB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8524_length_7989_cov_6.157298"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..1237
                     /locus_tag="PLZ74_07705"
     CDS             254..1237
                     /locus_tag="PLZ74_07705"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HOR98244.1"
                     /translation="MTLVEFIPPVPHPLWKLCFQMGITDVIIKVNPDLTGLADPWRYE
                     TLKKIVEGIASVGLRVVGLEGDPFDMSPIKEYGSKERGAADGEREEALAHYRELLESM
                     ARLDIKLLCYNFMVGAGWARSGVREGRGGAKATYFSSANVPDEPLRLTADQVWANYEH
                     FIKSVMPTAERCGVRMGLHPDDPCLPSLGGYARIFGSVEAYDRAYALYPSPSNAVTFC
                     QANFKLMGVDLEEAARHFGKRIAFIHVRDVEGTKEDFTELFHDQGTTDQFALMRTYRA
                     LGLDVPVRGDHVPEMEGDRLLTPEAIPGYFTLGRLFANGYLKALLRGTEHA"
     gene            1530..2444
                     /locus_tag="PLZ74_07710"
     CDS             1530..2444
                     /locus_tag="PLZ74_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOR98245.1"
                     /translation="MAGAFSAMFTPFTQDNRVNEDAIVQLIEYGIREGLKGFYLTGST
                     GEGLLLSIAERTLVYQTAVKAARGRCKLIAQVGAVRTEDSVALAKAAADAGCDWISSV
                     APVYFGQSFDAQYRHYKMISEATDLPFLVYCFNQNLVPERDIRLFDLKNVKGMKYTGS
                     DYYSVQKMRRNIDKETLFYAGRDEQLLCALALGDVFAGGIGLNYNMIPRHFAMICALA
                     AENDFDAAAKWQDEANRLVDLILEPGKWDNWSNFKVLMRHVGIDCGFCRAPYAPLSPN
                     EVLERLDAFDRLRIVEKNYACTPTMKET"
     gene            2447..4105
                     /locus_tag="PLZ74_07715"
     CDS             2447..4105
                     /locus_tag="PLZ74_07715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOR98246.1"
                     /translation="MRNCIFAFMALCGIALGAAEWTFPDGKDPACRLPPDPALAFPGG
                     FKATVRFSCDLGKIDRRSRFGNLFCRGVDFHDGYCVMVRFDGHLLIDILGVEPQYYIH
                     PMKLESNREYLLELYVTKSAVRVFIDGTETGSFCHEGTLEFAKKSMPLRIGSMGGYVF
                     FGSLPYLKLEALSEVTLPPGGPKPPLREAPRIQPRAEIKWSKTICKQEGCYIGWPTVC
                     RLKNGDLLAVFSGDRDGHICPWGKVQMVRSSDAGETWSEPVTIANTPLDDRDAGIVQM
                     PDGEIIVTWFTSMCWSRHMKGHPDWPRHLSKLAPETIKQWFGNLLIRSRDDGRTWSKP
                     EMLSNYTQTPHGPILLKDGALLQIGRTTRKVDDKAGGSFAKTIITVSKSKDAGRTWEV
                     ICPEIPAAPGENDAPSMFHEPHAVELADGTLVGLVRYHGDDHCMRATRSVDGGKTWTT
                     MEKTPMVGLPPHLIALPDGKLVNVYGRRIADPGYGEFACISDDGGTTWDVANEIMLHP
                     SFSGDLGYPASCLLPDGDILTVYYQKPTPKEKTALMATRWRVTR"
     gene            4229..4984
                     /locus_tag="PLZ74_07720"
     CDS             4229..4984
                     /locus_tag="PLZ74_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98247.1"
                     /translation="MKKLLQLLLGVACGALVSGEAVAVLPDPVIWWTMDELTEDGKIG
                     DASGNGRHLTLGPGMSLVDSRLSGKALASDGTVNTWATFSCPALTSRTISFWLYRNPA
                     DNDDSITSAENKYPYLFNGLSGMTLMFEGTTESFRQSVGNAGTAPFIFAGSLRTYWSH
                     HAITLEDTGHTDPTTGGAICQWNYYRNGVLTYAKTNLVATGSIAADSPTVIFMNQGTS
                     GNKRPVWGQIDEFRVVRHGALGRAGLRRIQASA"
     gene            4914..7364
                     /locus_tag="PLZ74_07725"
     CDS             4914..7364
                     /locus_tag="PLZ74_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98248.1"
                     /translation="MNSAWFDTVLSAEQVCAEFKRPLDARGPQLIGHWSMNEVTVHED
                     NSRTVAETTGLSNDLLLGSAVQHVNAPTGKGLYFDGTAASWAYTAVNNFQTLLDATIA
                     MWINIPVEAETLPADNKAPQIFSLSTEARAELQRERDRNDLRVFWHHNATARFLDPSL
                     AGRDVWSHLAFVFTHRYDAVAGKWYQTPRTYINGTLAATGAELEISNGAWVLEDPRAR
                     VTFGNNNKDAQRPIMGTLSDVSVWAGALSAEEIAELVHGPAAVDAGVDFVTTSQTAEL
                     RAALGNGDGEVVWSCVSGAGATILTPDAAVTQVLLPAEGAYTFRATVVGDLGTSSDEV
                     AVTRVAPLAVNVAPTVTLAATADVTLPAPLALAASVNDPDSQPGALRVKWTRLSGPGG
                     VWFEPPYAASTRATFTVAGTYVLACTVSDGDLETTAQTTVTVTADAASPGLAEGLILS
                     YTGDHGSPRREGVSGHLCVDNANYNGTNSSATFYADGKVGYGYKATGARAFFSSTRNL
                     LETVDPGAGQGNFWPAENFRTISVWIYYDTSSTNIVKQPLICGVPYTFTFALNVAADG
                     TGNGGFAVTQQGFRSGTSGLEWSREFWAPPSVSLENRWAHVCLVLDRKYGTGTEVYVD
                     GVLSSRTSGFIPGGRVVGSKFYIAGLDWSVADNNPTTVTGYFTGPGGSPEYSRAFPGI
                     VDEVKVWNRKLSVAEIAQLAAEPVPLKNRAPQVTVFPAGGPDSAIVRNRPVTLTATAF
                     DDGLPSSSSLAYEWQVVSGDAAGAAFSAQTAAATQVTVSSAGVYAFRVKVTDGDLTVY
                     SEPQAFAVVSAGTAILLR"
     gene            7533..>7989
                     /locus_tag="PLZ74_07730"
     CDS             7533..>7989
                     /locus_tag="PLZ74_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98249.1"
                     /translation="MSDLRQSLFKLLTATAFCLLSSGVFPLSAQQYSQEDIANRHTIA
                     FPNQRLQRDWVYQDLGSLDVKNVFTSKEANAQEKKLLGSVLRDLKALGLSAAAGKIEK
                     TFAALAGAGKPGADPAWQNLYFQACGVRRKERLARAFKDQPRTFIYAKHF"
BASE COUNT         1647 a         2363 c         2424 g         1555 t
ORIGIN      
        1 cgctcaagaa tattgcgtcg atcgccgcga aataccgctt ctcgtcgcct cagcactttg
       61 ccacggtctt caaacgcatc accggccaaa cgccgcgcac gtaccgcagc cgttgtttgg
      121 cagataacgg atagaacgtt ccaaaaaatt tccatatatt tgacatgttc tcaaatggga
      181 ttcttttttt gcccgactgg gaacgtaacc gaccgataac ctgatatact gtccccgcct
      241 atccatcgct tcaatgaccc tcgtagaatt cataccgcct gtgccgcatc cgctctggaa
      301 gctctgcttc cagatgggga tcacggacgt catcatcaag gtgaaccctg atttgacggg
      361 gctcgccgat ccgtggcgtt atgaaacgct caagaagatt gtcgaaggta tcgcgtccgt
      421 agggttgcgc gttgtcggcc tcgagggcga tccgtttgac atgtcgccga tcaaggaata
      481 cggaagcaag gaacgcggcg cggcggacgg ggaacgcgaa gaggcgctgg cgcactaccg
      541 cgagcttctg gagtcgatgg cgcggctcga catcaagctg ctctgctaca acttcatggt
      601 gggcgcgggg tgggcgcggt ccggggtgcg cgagggacgc ggcggcgcga aagcgacgta
      661 cttctcaagc gcgaacgtgc cggatgagcc gcttcgcctt acggcggacc aagtctgggc
      721 gaactacgaa cactttatta aatctgtgat gccgaccgca gagcggtgcg gcgtccgcat
      781 ggggctccat ccggacgatc cgtgtctccc gtcgctcggc ggttatgcgc ggatattcgg
      841 ttccgtcgag gcgtatgatc gtgcgtacgc gctttatccg tcaccgtcca atgccgtcac
      901 attctgccag gcgaacttca agctgatggg ggtcgacctt gaggaggcgg cgcggcattt
      961 cggcaagagg atcgcgttca ttcacgtacg cgacgtcgag ggaaccaaag aggatttcac
     1021 ggaacttttc cacgatcagg ggacgaccga ccaattcgcg ctcatgcgca catatcgtgc
     1081 gctcggactc gacgtgcccg tccgcggcga ccatgtgccg gagatggagg gcgaccggct
     1141 tttgacgccg gaagccatcc ccggttactt tacgctcggg cggctcttcg ccaacggcta
     1201 tctcaaagcc cttttgagag gaacggaaca tgcctgagtt tggccttgtc ggccggtaag
     1261 gcaccctgct ggaaatagac tagggcacca cgaattacgt gggcggcgtt tacgccgcca
     1321 ccacgggtga gatgcccgtg ccacacccag atacccgcca caaaacacct ttgtgccccg
     1381 ttgtagtggt tgtgtgagca aactcccgca accactacaa cagggcaaaa gtacaaaatt
     1441 tttttattca ggatcatgta caaacaacag tgacaaaatg ctatactacg ggcatgaagc
     1501 ccgaatcaca cggaaaagag tttgaaggaa tggcaggtgc gttttccgcc atgttcacgc
     1561 cgttcaccca agacaaccgg gtcaacgagg atgcgatcgt tcaactgatc gaatacggga
     1621 tacgggaggg cctgaagggg ttctacctga ccggttcgac cggcgagggt ctgcttctct
     1681 ctatcgcaga gcgcacgctg gtttaccaga cggccgtcaa ggccgcgcgg ggccgctgca
     1741 agttgattgc gcaggtcggt gcggtgagaa cggaggactc cgtcgcgctc gcgaaagcgg
     1801 cggcggatgc cggctgcgac tggatttcat ccgtggcgcc cgtgtatttc ggccagtcgt
     1861 tcgatgcgca gtatcgccac tacaagatga tctcggaggc gaccgacctg ccgtttctcg
     1921 tctactgctt taaccagaac ctcgtgcctg aacgtgacat ccgcctgttt gacctgaaga
     1981 acgtcaaggg catgaagtac accggcagtg attactacag cgtgcagaag atgcggcgga
     2041 acatcgacaa ggagacgctc ttctacgcgg gtcgtgacga gcagcttctc tgcgcgctgg
     2101 cgctcggtga cgtgttcgcg ggcggcatcg gcctcaacta caacatgatc ccccggcatt
     2161 ttgcgatgat ctgcgcgctc gcggcggaga acgacttcga tgcggcggcg aagtggcagg
     2221 acgaggcaaa ccggcttgtg gacctgatcc ttgagcccgg taaatgggac aactggtcca
     2281 acttcaaagt cctgatgcgt catgtgggta ttgactgcgg attctgccgt gcgccttacg
     2341 cgccactctc tccaaatgag gtgctcgaac gcctcgacgc attcgaccgc ttgaggatcg
     2401 tagagaaaaa ctacgcctgc acgccaacca tgaaggagac ttaaacatga gaaactgtat
     2461 cttcgctttc atggccctgt gcggtatcgc tctgggcgcc gccgaatgga ccttcccgga
     2521 cggcaaggac cctgcctgcc gcttgccgcc tgatccggcg ctcgccttcc cgggcggctt
     2581 caaagcaacg gtgcgtttct cctgcgacct cggcaaaatc gaccgccgct cacgcttcgg
     2641 aaatctgttt tgtcgtggcg tcgacttcca tgacggctat tgcgtgatgg tccgcttcga
     2701 cggccacctg ctcatcgaca tcctcggcgt cgagccgcag tactacatac acccgatgaa
     2761 actcgaatcc aaccgagagt atctgctgga actttacgtg acgaagagcg ccgtgcgcgt
     2821 cttcatcgac gggacggaga cggggagttt ctgtcacgaa ggcacgctgg agttcgcgaa
     2881 aaaatcaatg ccgctcagaa tcgggtcgat gggcggttac gtgtttttcg gttcactacc
     2941 atacctgaag cttgaagcgc tttcagaagt cacgttaccg ccgggcggcc ccaagccgcc
     3001 gctccgtgaa gcgccgcgca tccagccgcg cgcggagatc aagtggtcga agaccatctg
     3061 caagcaggaa gggtgctaca tcggctggcc gaccgtctgc cgtctcaaaa atggcgacct
     3121 cctcgcggtg ttctccggtg accgcgacgg acacatctgc ccctggggca aagtccagat
     3181 ggtgcgctcc tcggatgcgg gcgaaacctg gagtgagccc gtgacgatcg cgaatacccc
     3241 gctggatgat cgcgacgcgg gtatcgtcca gatgccggac ggggaaatca tcgtgacctg
     3301 gttcacctcc atgtgctggt cgcgacacat gaaggggcac cccgactggc cgcgccacct
     3361 ctccaaactc gcgccggaaa cgatcaagca atggttcggc aaccttctca tccgttcgcg
     3421 cgatgacggg cgcacatgga gcaagcccga aatgctgtcg aactacaccc agaccccgca
     3481 cggaccgatc ctcctcaagg acggcgccct gctgcagatc gggcgcacca ccagaaaggt
     3541 ggacgacaaa gcgggcggaa gtttcgccaa aacgatcatc acggtttcca aatccaagga
     3601 cgccggccgc acctgggagg tgatctgtcc ggaaatcccg gccgcgcccg gcgaaaacga
     3661 cgcacccagc atgttccatg agccgcacgc cgtcgagctg gccgacggca cgctggtcgg
     3721 gctcgtgcgg tatcatggcg acgaccattg catgcgcgcg acgcggtccg tagatggcgg
     3781 caaaacgtgg acgacgatgg agaagacgcc gatggtcggc ctgccgccgc acctgatcgc
     3841 gttgccggat ggcaagcttg taaatgttta cggacgcaga atcgcggacc ccggttatgg
     3901 agagttcgcc tgtatttcag atgacggcgg cacgacgtgg gatgtcgcaa acgagattat
     3961 gctccatcct tcattcagcg gcgatctcgg atatcccgcc tcctgtctac tgccggacgg
     4021 tgacatcctc accgtgtatt accagaaacc cacgcctaag gagaagaccg cgctcatggc
     4081 cacgcggtgg cgtgtgacac gataacaaac gatgatgagg ccagagtaac aaccagaggc
     4141 tcttgaaaaa ctctcgtttc atgggcgtcc cgcccatgaa acgcgggcaa gatgccgtgc
     4201 cacaaccaga gcaagaaagg aaagcacaat gaaaaaactg ttgcaactgt tgctaggtgt
     4261 agcttgcggc gcactcgtat cgggtgaagc cgttgccgtg ttgccagatc ctgtgatctg
     4321 gtggacgatg gacgaattga ctgaagacgg aaagatcggc gacgcgagcg ggaacgggcg
     4381 tcacctgacg ctcggccccg gcatgtcgtt ggtggacagc cgtctctcgg gcaaggcgct
     4441 cgcctccgac ggaacggtga acacatgggc caccttctcg tgcccggccc tcacgagccg
     4501 cacgatctcg ttttggctct accgcaatcc ggcggacaat gacgattcga taacgagcgc
     4561 agaaaacaag tacccctatc tcttcaatgg tctgtcaggc atgaccctca tgttcgaggg
     4621 cacgacggaa agcttccggc agagcgtggg taacgcaggg acagcacctt ttattttcgc
     4681 gggttccttg cggacctatt ggagccacca tgcgatcacg ctggaagata cgggacatac
     4741 agacccaaca acaggcggcg cgatctgtca atggaattac taccgtaacg gtgtgctgac
     4801 ttatgctaag actaatcttg tcgccacagg ttcgatcgcg gccgacagcc ctaccgtgat
     4861 cttcatgaac caaggcacct cgggtaataa gcgccccgtt tggggacaga ttgatgaatt
     4921 ccgcgtggtt cgacacggtg ctctcggccg agcaggtctg cgccgaattc aagcgtccgc
     4981 ttgacgcgcg cggaccgcaa ctaatcggtc actggagcat gaacgaggtg acggtgcatg
     5041 aggacaactc gcgcaccgtg gctgaaacga ccggactctc taatgacctc ctgctcgggt
     5101 cggccgtgca gcatgtgaac gcgcccaccg gcaagggact ctatttcgat ggtacggcgg
     5161 cgtcttgggc ctataccgcc gtgaacaact tccagacgct gctcgatgca acgatcgcga
     5221 tgtggattaa tatccccgtc gaagcggaga cgctccccgc agacaacaaa gccccgcaga
     5281 tcttttcgct gagtacggag gcccgcgccg aactccagcg ggagcgcgac agaaacgacc
     5341 tccgtgtgtt ctggcaccac aacgcaactg cgcggttctt agacccctcc ctcgccggac
     5401 gtgacgtgtg gagccatctc gcgtttgtgt tcacgcaccg ctatgatgcc gtagcgggca
     5461 agtggtatca gacgccacgc acttacatca acggtacgct tgccgcgacc ggagcagagc
     5521 tagagatatc gaatggtgcc tgggtgcttg aagatccccg tgcacgtgtc acgtttggca
     5581 ataacaataa agatgctcag agaccgatca tgggaaccct ttctgacgtg agcgtctggg
     5641 ccggcgcact gtctgctgag gagatcgccg aactggtaca cggcccggca gcggttgatg
     5701 cgggcgtcga ttttgtcacc acgtcgcaga cggccgagtt gcgcgctgca ctcggtaacg
     5761 gtgacggcga ggttgtctgg tcgtgcgtct ccggcgcggg ggcgacgatc ctcacgcccg
     5821 acgctgcggt gacgcaggtg ttgctgcccg cagagggcgc ctacaccttc cgtgcgactg
     5881 tcgtggggga tctgggcacg agtagcgacg aggtcgccgt gacgcgggtc gcgccactgg
     5941 cggtgaacgt agcgccgacg gtgacgcttg ccgcgacggc ggacgtcacg ttgccagcgc
     6001 cactcgcgct tgcagcatcc gttaacgacc ccgattcaca gccaggcgca ctccgtgtga
     6061 aatggacgcg cctgtcgggg ccgggcggcg tgtggtttga gccgccgtat gccgcttcga
     6121 cgcgcgcgac attcaccgta gccggaacgt acgtgctcgc ctgcacggtt tcggacggcg
     6181 accttgagac gaccgcgcag accacggtca ccgtcacagc ggatgccgcg tcgccggggt
     6241 tggcggaagg cctgatcttg agctacacgg gtgaccacgg atcaccgcgc cgcgagggcg
     6301 tttcagggca cttgtgtgtg gataatgcca actacaacgg cacgaacagc agcgcgacgt
     6361 tttatgcgga cggcaaagtc ggctacggct ataaagcgac aggcgcacgc gcctttttct
     6421 cgtcgaccag gaatctgctg gagacggtcg accctggggc gggacaagga aacttctggc
     6481 ctgccgagaa cttccggacg atcagtgtct ggatctacta cgacacgtcc tccaccaaca
     6541 tcgttaaaca gccgctgatt tgcggcgttc cctacacatt cacgttcgcc ctgaatgtgg
     6601 ccgctgacgg tacaggaaat ggcgggtttg ccgtcacgca acaggggttc aggagcggaa
     6661 cttcaggtct tgaatggagc cgcgagttct gggcaccgcc gagcgtttcg cttgaaaatc
     6721 gctgggcgca tgtctgcctt gttcttgacc gcaaatatgg gacgggtacc gaggtctacg
     6781 tggatggtgt actgtccagc cgtacgagcg gtttcattcc cggcgggcgc gtggtcggca
     6841 gcaaattcta tatcgccgga ttggattgga gtgtagcgga caacaacccg actacggtga
     6901 cgggctattt cacggggcct ggcggcagtc ctgagtactc gcgcgccttc ccggggatcg
     6961 tcgatgaagt gaaggtgtgg aaccgcaagc tttcggttgc tgagatcgct cagcttgcgg
     7021 cggaacccgt cccgctgaag aaccgcgccc cgcaggtcac cgtctttcct gccggaggac
     7081 cggacagcgc aatcgtgcgc aatcgcccag tgacgctcac ggcgacggcg tttgacgacg
     7141 ggctgccgtc aagttcatcg ctcgcctatg aatggcaggt ggtttccgga gatgcggcgg
     7201 gcgcggcatt ctctgcgcaa accgccgccg ccacgcaggt gacggtgagc agtgcgggtg
     7261 tgtatgcttt ccgcgtcaaa gtcactgacg gcgacttgac cgtgtacagc gagccgcagg
     7321 cgttcgcggt cgtttcggcg ggaacagcca ttctgctccg gtagcggcaa ccttgatccc
     7381 gccacctata attccgcgcg agtcggcagc tacttgccta atgatggcat aagcgtctca
     7441 ggttcagact tgtcaatgcg gtgcaataag tgtatcaaac gactgtcctg gctggggcag
     7501 gtgattccgc ttttcgtaaa tagggggggc gcatgtctga tctgaggcaa tcgttgttca
     7561 aactgttgac ggccaccgcc ttctgcctgc tgtcttccgg cgtctttccg ctgtccgccc
     7621 aacaatattc ccaagaggac atcgcgaacc gccacacaat cgcgtttccg aaccagcgcc
     7681 tccagcggga ttgggtctac caggacctcg gctcgctgga cgtgaagaat gttttcacat
     7741 ccaaggaggc caacgctcag gaaaagaagc tgttgggctc cgtgctgaga gatcttaaag
     7801 ccctcggcct cagcgcggcg gccggaaaaa tcgaaaaaac gtttgcggcg ctggccggag
     7861 cgggaaagcc gggggccgac cccgcctggc agaacctcta tttccaagcg tgcggcgtgc
     7921 gccgcaaaga gcgcctggcg cgcgcgttca aggaccagcc gcggacgttc atctatgcca
     7981 aacattttg
//
LOCUS       DAOSRB010000130         7962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8563_length_7962_cov_5.846718, whole genome shotgun sequence.
ACCESSION   DAOSRB010000130 DAOSRB010000000
VERSION     DAOSRB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8563_length_7962_cov_5.846718"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..881)
                     /locus_tag="PLZ74_07735"
     CDS             complement(81..881)
                     /locus_tag="PLZ74_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOR98250.1"
                     /translation="MTYTPSASPSNVRGIICDMDGVIYHGNALLPGTRPFIDWLRQSK
                     TPFLFLTNSSERSPRELSEKLSRLGVLIEESHFYTSAMATAAFLASQTPGGSAYVIGQ
                     PGLIHALYEAGFSINDVNPDYVVVGETPMYTYETIKKSVSLVRNGAKLIGTNPDLTGP
                     VEHGIIPACGALVAPIELATGMKAYFVGKPNPLMMRSALRLLGCPRESALIVGDRMDT
                     DILSGIEAGMQTVLVLSGVTRREDLVRYSYRPNHVLDHVGLLPDLLGG"
     gene            1174..1956
                     /locus_tag="PLZ74_07740"
     CDS             1174..1956
                     /locus_tag="PLZ74_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR98251.1"
                     /translation="METNKRKLLLVDDDPSLLETLGDFLMFEGYEVLCAASGEDALVK
                     MRAFQPDLIILDMGMPGMGGTGFLDRITQPDGVPLYPVLVLTARASMAEYFADKQISG
                     FIAKPCDPSDLLMEVSRILFMSPADGRHAASQRVVRRLVIGEGDAALRETLRREFTLA
                     GFEVEVCATGPETLEACITLKPHAVVMRLELDGMSADEVVGMLHRLPGARQIRAVVYG
                     MDLPEAQLEHVANLDIPQSGMIRDLTVNTILDRTMAVTGGAA"
     gene            1982..2428
                     /gene="nrdR"
                     /locus_tag="PLZ74_07745"
     CDS             1982..2428
                     /gene="nrdR"
                     /locus_tag="PLZ74_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOR98252.1"
                     /translation="MRCPKCGHLDDKVLDSRAAREGASIRRRRQCLACEHRFTTYEEI
                     VKDELRVIKRDGRREEFSRLKLERGVMRACEKRPISADQIHDLVDEIIEGFEGGSEVP
                     SERIGQAVMTRLHKLDEVAYIRFASVYRRFADVSQFLKAVQDMVES"
     gene            2444..2761
                     /locus_tag="PLZ74_07750"
     CDS             2444..2761
                     /locus_tag="PLZ74_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HOR98253.1"
                     /translation="MLKLTVYIPETHLESVKAALFAAGAGRYAAYDHCCWQVLGEGQF
                     RPLAGSSPYIGNTGSVTRVAEYRVEMICAEAECAAVTAALRAAHPYEEPAFDFVRLCE
                     GSG"
     gene            complement(2851..4158)
                     /locus_tag="PLZ74_07755"
     CDS             complement(2851..4158)
                     /locus_tag="PLZ74_07755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOR98254.1"
                     /translation="MVSVIDLWQYEGIDFLQRYVGAKFGLLGIEPYHATADWWLNGID
                     KAFHETHFKGVPRPPGVGCNSPCLLLFYMPFTWFDFTTARYVSGALEWLAFLSVLCLM
                     TRLIPRNRDRALTFSLILFFTAGAPFWRLHVERGQYYVFTCLLIASSLVAHIKSNRPF
                     LSGLLLGLTVVIRPTVAFLALPFALQRRWRMLTGIACACLFMGGASLAVFGVEGWRQF
                     IWISEKYEIASTDFETDHFADLLRGRDPLPIQDFEQSRKQGLAMLPSINSTAAGMVRL
                     SKPLLDRCSIPVTSVLFLAKLVGVSTAVALILAALYTRSRTPWPFAYLAYVGIVAVNL
                     VEYAIPYRAAYADIYYLLPVMLGIPIVLRHRSVWLGVLLLVALASQQNLLGLVHGWYA
                     IPVRVAGLTLFSLGVLWHWRQSIISAPSAFDVTGETGSSSVPA"
     gene            complement(4317..5090)
                     /locus_tag="PLZ74_07760"
     CDS             complement(4317..5090)
                     /locus_tag="PLZ74_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR98255.1"
                     /translation="MTKKKIVVVMPAYNAAKTLKKTYDEVMCQGIVDEIILVDDASTD
                     ETVTLAATLPKVRVHVHPKNAGYGANQKTCYRLALESGADIVVMVHPDYQYTPKLIPA
                     MVSLIGSGLYPCVLGSRILGGYALKGGMPFWKYFSNRALTLFMNLLFGSKLSEFHTGY
                     RAFSRSLLERLPLQANMDDFAFDAQMLAQILWSGALIGEISCPTRYFEEASSINFQRS
                     VKYGFGCLATALEYRFCKEKLLSSHRFPRELSENAGRKP"
     gene            complement(5145..5315)
                     /locus_tag="PLZ74_07765"
     CDS             complement(5145..5315)
                     /locus_tag="PLZ74_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98256.1"
                     /translation="MRKPGGIGSKEPSCELGLLAAQNLCKGTCHDLWAINTDYEYQEM
                     SRITETGLIQIV"
     gene            5407..>7962
                     /locus_tag="PLZ74_07770"
     CDS             5407..>7962
                     /locus_tag="PLZ74_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98257.1"
                     /translation="MKKRFRFSGGSRWLEGLGLLFVMLLFWGSMLYVFYPVLSRDWVM
                     LSPDFPKFYPTCAQAKWLEMVLAGRLSVVPMHALKFLGHPLIWQEFFFMLSTGLAGVA
                     VAYYLRTQRVPLVPACGGGLCFALSGYSFTLFCAGHGGYFYLISCGLFAFGLVNRCFQ
                     TRAFFYFALLGAVLMWAEIEQPDIWLLFVMLLAAYTLWRSYREWRESGSGSFIWRVVP
                     RFFLTLAVMLLIGFQQIRSAFTESLMNRTLQMEEHSSVTEQEQKTDPVQAEARKREER
                     WIFATNWSLPPEDMLEFIIPSLFGNDSFHMPHPYWGRLGRPHASVFQKGRMMPNYRQH
                     VVYLGVVAVLLAIFALFAWCAARRQEETGNQRTDDAGGPTGQGRQATRVSVFADVPFW
                     VGVWGVCAMISLGRYTPFYRLVYALPFMEYLRAPVKFHHLVELANACLAGWGLYALSS
                     SDVSTRRLRQRFAVFAAGVAGCLVVLALVLQVGAAGMERHITELGMGQVASALRGFAV
                     ANTVRSALLTLAVAGVFAGSAWRGVSLRRSLFCVCLLTALNTADLAVVARRCVTPVNV
                     GPHHAANLVVKTMLSRTGGRPANVLNYVTSNVWDQDWFHTALELHGFACVGPDPQNRS
                     APRSLLFEAFQRSPLRYWQVMGARFVLLPRKHAEPFFRQNILTAVCDFEIGRDTVRTS
                     QPTETSLVLAEVKGLPALPALYFEWEGPVAGDQQLQAVRAKESVGHLVADVDGVRGVA
                     TGSAVAPRSVVLDTLRKMPRALETRGLVETDRPALLVLGETYDAGLEALVDRVPVRVC
                     QANGMWAAVPVPAGKHEIVVRMKRNWGLNALSVATSLTLLGWFAVRLARRNVGR"
BASE COUNT         1628 a         2042 c         2567 g         1725 t
ORIGIN      
        1 gaaaccctga aaccatgaaa ccctgaaacc aaagagagtt gaaggttcaa ctgctctttt
       61 taggttaaac cgcctggcgg tcagccgccg agcagatccg gcagtagacc gacgtgatcc
      121 agcacatggt tggggcggta gctgtaccgg acgagatcct cgcgtcgtgt cacgccggag
      181 agcaccagaa ccgtttgcat gcccgcctcg attccggaaa ggatgtccgt gtccatacgg
      241 tccccgacga tcagggcgga ttcgcgcggg caccccagca ggcggagcgc cgagcgcatc
      301 atcagcggat tgggtttgcc tacgaaatag gctttcatgc cggtggccag ttcaatcggg
      361 gccaccagcg cgccgcaggc cgggatgatg ccgtgttcga ccggtccggt caaatcgggg
      421 ttggtgccga ttagtttggc gccgttacgc acgagtgaga cggatttctt gatggtctcg
      481 taggtgtaca tgggggtttc gcccaccacc acgtagtccg ggttgacgtc gttgatggag
      541 aaccccgcct cgtagagcgc gtggattagg ccgggttggc cgatcacata cgcgctgccg
      601 cccggcgtct gggatgcgag gaaggcggcg gtcgccatgg cgctcgtata gaaatgcgac
      661 tcttcgatca agacgcccaa tcgcgaaagt ttctcgctga gttcccgcgg cgaacgttcg
      721 ctgctgttgg tcaggaacag gaagggagtt ttggattggc gcagccagtc gataaacggc
      781 cgcgtgccgg gtaacagcgc gtttccgtga tagatcacgc cgtccatatc acagatgatg
      841 ccgcggacat tggaagggga tgcggagggg gtgtaggtca tagggactcc tggagggcgt
      901 tgaaatgcgt acatgtgcca tacacgggga attggaccca aagcgcgtct aactcctctt
      961 gcatggcggc caccaaagcg attggttttt gcatgccggt cagtttcgta tgatttggag
     1021 agagtgtcaa ccgtttgcgg tggcctgtca acaattcgac gtttgcataa agcatcttat
     1081 ggcgcgatta caaagaaaac tcgatatggg tgaattcggt tgtaacctta tccccttgaa
     1141 accgttgcgg atacgggata cactctccgg catatggaaa cgaacaaacg caaactgttg
     1201 ttggtggatg atgacccgag cctgctcgaa acgttgggcg actttctgat gtttgagggg
     1261 tacgaggtgc tgtgtgcggc cagcggggag gatgcgctgg tcaaaatgcg ggcgttccag
     1321 ccggatctca tcattcttga catgggcatg ccagggatgg gcggcacggg attcctcgac
     1381 cggatcaccc agccggacgg ggtgccgctc tatccggttc tggtgctgac ggcgcgcgcc
     1441 tcgatggcgg agtatttcgc cgacaaacag atatcgggat tcattgcgaa accgtgcgat
     1501 ccctccgatc tgctgatgga ggtcagccgg attctcttca tgagtccggc ggacggccgg
     1561 catgcggcat cccagcgggt ggtgcggcgg ctggtgatcg gcgaagggga cgccgccttg
     1621 cgcgagacac tgcgccggga gtttacgctc gccggattcg aggtggaggt ttgcgctacc
     1681 gggccggaaa cgctcgaggc gtgtatcacg ctcaagcccc atgcggttgt gatgcggctg
     1741 gaactcgatg ggatgagcgc ggacgaagtg gtcggcatgc tgcaccggtt gccgggcgcg
     1801 cggcagatcc gggcggtggt gtacgggatg gatcttcccg aggcgcaact ggagcatgtg
     1861 gcgaatctgg atattccgca gagcggaatg atccgggatt tgactgtgaa tacgattctt
     1921 gatcggacta tggctgttac tggcggcgcc gcctgacaaa gaaggcggaa agggaggtgc
     1981 gatgcgttgt cccaaatgcg ggcatttgga cgataaggtg ttggacagcc gggcggcgcg
     2041 cgaaggcgcg tcgatccggc ggcgacgcca gtgtctggcg tgcgaacatc ggttcaccac
     2101 gtatgaggag atcgttaaag acgagttacg ggtgattaag cgggacggac gccgggagga
     2161 gttcagccgt ctgaagttgg agcgcggagt gatgcgggcg tgtgagaagc gtcccatcag
     2221 cgcggatcag attcacgatc tggtggacga gatcatcgaa gggttcgagg gtgggagcga
     2281 agttccctcg gaacgaatcg ggcaggcggt gatgacgcgt ttgcacaaac tggatgaggt
     2341 ggcctatatt cgttttgcct cggtctaccg tcgttttgcg gatgtgagcc aatttctcaa
     2401 ggcggttcaa gatatggtcg agtcgtagcc ccggccgtca gatatgctga aattaaccgt
     2461 atatattccg gaaacgcatc tggagtcggt gaaagcggcg ctcttcgccg ctggagcggg
     2521 acgttacgcc gcatatgacc actgttgctg gcaggtgctg ggcgagggac agttccgtcc
     2581 gcttgcgggc agttcaccgt atatcgggaa tacaggctcc gtgacgcgcg tagccgaata
     2641 tcgggtggag atgatctgcg cggaagcgga gtgtgcggcg gtcacggcgg cgttacgcgc
     2701 cgcgcatccg tatgaggagc cggcatttga ttttgtgagg ctctgcgaag ggtccggatg
     2761 aggggaacgg catgaccatc cgcgagcatc tggatgaggc ggtttgtttg gcgcccgagg
     2821 cacgtccacg atgaaagtcc ggcgggtggc ttatgccggg acgcttgatg agccggtctc
     2881 tccggtaacg tcaaacgccg acggcgcgga gatgatgctc tgacgccagt gccagagcac
     2941 cccgagggaa aaaagcgtca gtcccgcaac ccgcaccggg atcgcgtacc atccgtgcac
     3001 cagtccaagc aggttctgct gtgaggcgag cgcgaccaac agcaggacgc cgagccatac
     3061 gctccggtgg cggaggacaa tcgggatgcc gagcatgacc ggcagcaggt aatagatgtc
     3121 cgcataggcc gcgcggtagg ggatggcgta ttcaaccaga ttcacggcca cgatgcccac
     3181 gtaggcgaga taggcgaacg gccaaggcgt acgggagcgg gtatagaggg cggcaaggat
     3241 aagtgcgaca gccgtactga cgccgacaag tttggccagg aaaagcacgc ttgtgacggg
     3301 aatcgaacag cgatcaagca acggtttgga gagacgcacc attcctgcgg cggtgctgtt
     3361 tatggacggc aacatcgcca atccttgttt ccgcgactgc tcgaaatctt ggatcggcaa
     3421 ggggtcgcgg ccgcgaagca agtcggcaaa atgatccgtc tcaaaatcag ttgaggcgat
     3481 ttcatatttc tcggaaatcc aaatgaactg gcgccagcct tcgaccccga acacggctag
     3541 cgacgctcct cccatgaaca aacacgcgca agcaatgccc gtcagcatac gccatcggcg
     3601 ttgcagggcg aacggcaacg cgagaaatgc gacggtgggg cggattacga cggtgagtcc
     3661 gagaaggagt cctgacagga aaggacggtt cgacttgatg tgcgccacca gtgaactggc
     3721 gatcagcaga caggtaaaca cgtagtattg tccccgttca acgtgcagac gccagaaggg
     3781 cgcacctgcc gtaaagaaaa gaatcaggga gaaagtcagt gcgcgatccc tgtttcgagg
     3841 aatcaaacgg gtcatcaggc aaaggacgga caggaacgcc aaccattcga gtgcaccact
     3901 cacatatcgc gccgtcgtga aatcgaacca agtaaacggc atataaaaca acaaaaggca
     3961 gggcgaattg catccgacac cgggcgggcg cgggactcct ttgaaatggg tttcatgaaa
     4021 cgctttgtcg ataccgttca accaccagtc tgccgtggcg tggtagggtt ctatacccaa
     4081 caagccgaat tttgcgccga cgtagcgttg caggaaatca atgccctcgt actgccagag
     4141 atcgatgacc gaaaccacga ggcttgcgaa caagacaaac agagcggaca ggaagaagac
     4201 gcgaagccaa aaaggaagag gggtcagtcg gaaaccgaac acggtggcac acaacgagtc
     4261 tgtcttgttg catcttatgg ccagaggcaa agaaggttgt gggaaagcgc cttcgttcat
     4321 ggttttcttc ctgcgttttc agatagttct cgcggaaaac ggtgagatga aagaagcttt
     4381 tctttacaga atcgatactc caaggcggtc gccagacaac cgaagccgta ttttacgctt
     4441 ctttggaaat tgatcgagga ggcttcctca aaataacgtg tcgggcagga gatttcgccg
     4501 atgagggctc cgctccagag gatttgcgcg agcatctgcg cgtcaaacgc gaaatcatcc
     4561 atgttcgcct gtaaggggag gcgctcgaga agcgaacgcg aaaaagcgcg gtatccggta
     4621 tggaactcag acagttttga tccgaaaagt aaattcatga acagggtcag cgctcgattg
     4681 gaaaaatatt tccaaaaagg cattccccct ttgagggcgt agccccccag aatgcgcgag
     4741 ccgagcacac acggatagag accggacccg atcaggctca ccatggctgg aatcagtttg
     4801 ggagtgtatt ggtaatccgg gtgcaccatg acaacgatgt ccgcgccgga ctcgagggcc
     4861 agccggtaac acgttttctg attcgccccg tatccggcgt ttttcgggtg tacatggact
     4921 cgtaccttcg gcagggtcgc agcaagagtc acggtctcgt ccgtactggc atcatccaca
     4981 agaataatct catcgacaat cccttggcac atgacttcat cataggtttt cttgagtgtt
     5041 ttcgcggcat tatacgccgg catgacgaca acgatctttt ttttggtcat ctcatatcct
     5101 tctgtcttaa gagggtaaaa aactccttcc actggacgag tcggctacac gatctggatc
     5161 aatcctgttt ccgtgatacg ggacatctcc tgatattcgt aatccgtgtt gatggcccaa
     5221 agatcatggc acgtaccctt gcataggttc tgggccgcca gcaaccccaa ctcgcatgat
     5281 ggctcctttg agccgattcc gccgggcttc ctcaccacgg aagtcccgaa aattcggatc
     5341 ggatcacgaa aaatagtata ggcattgacg gtgcggattg ctaatataaa aacaaaagga
     5401 tttacaatga agaagcgttt ccggttttca ggcggttcca gatggctcga aggcctgggg
     5461 ctcctgttcg ttatgctcct gttctggggc agtatgctct atgtcttcta tcctgttctg
     5521 tcccgagact gggtgatgct gtcgccggat ttcccgaagt tctatccgac ctgcgcgcaa
     5581 gccaaatggt tggaaatggt tttggcgggc cgcctttcgg tggtgccgat gcacgcgcta
     5641 aaatttctcg ggcatccgct gatctggcag gagttctttt ttatgctctc cacagggctt
     5701 gccggagtcg cggtggccta ttatctgcgc actcagcgcg ttcctcttgt accggcttgc
     5761 ggcgggggat tgtgtttcgc cttgtccggg tattcgttca ccctgttctg tgccgggcac
     5821 ggcggatatt tctacctgat ctcctgcggt ctgttcgcat tcggccttgt gaaccggtgt
     5881 ttccaaaccc gggcgttctt ttatttcgct ttactcggtg ccgttctgat gtgggcggag
     5941 atcgaacaac cggatatttg gttgttgttt gtcatgctgt tggccgcata tacgctttgg
     6001 cgatcttacc gggagtggcg ggagtcgggg agcggttcgt ttatctggcg cgtggttccg
     6061 cggtttttcc tgacccttgc cgtcatgctt ctgatcggtt ttcaacaaat ccgttcggct
     6121 ttcaccgagt cgctgatgaa ccgcaccctg cagatggaag aacactcttc tgtcacggag
     6181 caggaacaaa agacggaccc tgtgcaagcg gaggctcgga aacgcgaaga acgctggatt
     6241 tttgcgacca actggagtct gcccccggag gacatgctcg aattcatcat ccccagtctg
     6301 ttcgggaatg actccttcca catgccgcat ccctattggg ggaggctggg acgtccgcat
     6361 gcgtccgtgt ttcaaaaagg gcgcatgatg ccgaactacc ggcagcatgt agtgtatctg
     6421 ggggtggtgg cggttctgct tgcgatcttt gcgctgttcg cgtggtgcgc cgcccgtcgg
     6481 caggaggaaa cggggaatca aagaacggat gatgcgggcg gcccgacggg acaggggagg
     6541 caggccacaa gggtgtcggt ctttgccgac gtgccgttct gggtcggggt ttggggggtc
     6601 tgcgcgatga tctctctggg acggtacacg ccgttctata ggctggtcta tgctttgccc
     6661 ttcatggaat atttgcgcgc gcctgtcaaa tttcatcatc tggtggaact ggcgaacgcc
     6721 tgtctggccg ggtggggact ttatgcgctc tcgagttcgg atgtctcgac acggcggctc
     6781 cggcaacggt ttgccgtgtt cgcggcaggt gtggcaggat gtctggtcgt tttggcgttg
     6841 gttcttcagg tcggggctgc gggaatggaa cggcatatca cggagttggg gatggggcaa
     6901 gttgcgtccg ctttacgagg gtttgcggta gccaacacgg tccggtccgc gctcctgacg
     6961 cttgccgttg ctggtgtttt cgccgggtct gcctggcgcg gtgtctcctt aaggcgaagt
     7021 ctgttctgcg tctgtttgct gaccgcattg aacacggccg atttggctgt cgtcgcgcga
     7081 cgttgcgtca ccccggtgaa tgtcgggccg catcatgccg cgaatctcgt ggtaaagacg
     7141 atgctgtcgc gtaccggggg acgacccgcg aacgtcctca actatgtcac ctccaacgtg
     7201 tgggatcagg attggtttca cacggcgctg gaattgcacg gattcgcctg cgtggggcct
     7261 gatccgcaga accggagcgc gccgcgctcg ctgttgtttg aggcgtttca gcgttctccc
     7321 ctgcgatact ggcaggtcat gggggcacgg ttcgtgctgt tgccgcgaaa acacgctgaa
     7381 ccgtttttcc gccagaacat ccttaccgct gtatgtgatt ttgagatcgg gcgtgacacg
     7441 gtccggacaa gtcagcccac ggaaacgagt ctggtgctgg cggaggtgaa gggtctgccc
     7501 gccttgccgg cattgtactt cgagtgggag ggtccggtag cgggtgatca acaactgcag
     7561 gcggtacgcg caaaggagtc ggtcgggcat ctggtggccg atgtggatgg ggtgcggggt
     7621 gtggcgacgg ggagtgcggt ggcgccacgc agtgtcgtgt tggacaccct gcggaagatg
     7681 ccgcgtgcgc tggagacgcg cggtctggtt gaaacggacc gtcctgccct gctggtgtta
     7741 ggggagacgt atgacgcggg attggaggcc cttgtcgacc gcgtgccggt acgtgtgtgt
     7801 caagccaacg ggatgtgggc cgcagttccc gtgcctgccg gcaaacatga gattgtggtg
     7861 cgcatgaaac ggaactgggg gttgaatgcc ctcagtgtgg cgacgagtct gacacttctc
     7921 ggctggtttg cggtacgtct cgcgcgaagg aatgtggggc gg
//
LOCUS       DAOSRB010000131         7867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8693_length_7867_cov_6.962750, whole genome shotgun sequence.
ACCESSION   DAOSRB010000131 DAOSRB010000000
VERSION     DAOSRB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8693_length_7867_cov_6.962750"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1415)
                     /locus_tag="PLZ74_07775"
     CDS             complement(72..1415)
                     /locus_tag="PLZ74_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR98258.1"
                     /translation="MITNLFVLLGFAAVCSVFNGCALLRRRTLAPWAFALLYGVMGVV
                     VMLTPVVTHSGLTVDCRGGVLGTAALLIGPPVALICLLPPLAYRLALGSPHLLLGVGS
                     LVFPVLLGTLANLWQRRHDLSLTPRLVLSLSLLVGLGSNLLLALFAFCEGRGAVFDVG
                     VARALAALWITPVSMALLSALVLSEQRHITTVEQLAETERRMLGSQKMAAIGQLAYRV
                     AHTMLNALTVIQNRAELTKADPRSPKRVVANMDEVIRTVGTVSNLMGELVAFAIPDAL
                     RLRQMRLDTCLEGVERLLGKTIGEEVELVIRQGDVADGKVTIDPNRIEQLIVHLAINA
                     VEAVAGRGQVTISTYEISLSEEERTRLMAGLPACERHQGRFAVLEVRDTGCGMTAETA
                     GRIFEPFFTTKADRKNAGLGLPTVYAIVQQHHGFIDVQTQPGCGSTFLIYLPVTG"
     gene            complement(1476..2360)
                     /locus_tag="PLZ74_07780"
     CDS             complement(1476..2360)
                     /locus_tag="PLZ74_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOR98259.1"
                     /translation="MIMPKNADRLLMAFCPSANIVLRHAEVTDSARELEHSHLCGPTA
                     GLIQAEALAGVALLGAELTRPEEAVTLRLQVTGPIGGVLVEARSDGSLRGYTQVKVMN
                     DLDACEDLDAAVALGDSAEVRVIRSLPGVLLSSAGVEVRPASVPQGIETYYRQSQQRR
                     VRVQLTALAYGGFIDCARGVLIECLPDGDRAEFERIGRFVEDGTVQECLEAGASLQTI
                     CETVGVPDVQLAEPKPLRFHCPCSAERVSDMLGSMPTKELEEMICADQSVRVICHMCG
                     KGYSVAVEELKRIVASRS"
     gene            complement(2357..4471)
                     /locus_tag="PLZ74_07785"
     CDS             complement(2357..4471)
                     /locus_tag="PLZ74_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98260.1"
                     /translation="MKKTVHQFIARVRREARQKTEPVTVRSMGRDRVFYGYDWGVDTF
                     IFEKDVWKNLEEHSPVLIVRKSELIRRSGGHILTLTSGNHTVAAIPLLSGHFWNLGRV
                     PAARRSQVMREEVLCANVVNGNLEISQRDVPTRLLIKADEWLRNVGFALDEVIMAERT
                     DMTLDHYRRLGQEWRIKPLAWTRREMDAALRASRTRINTCLKYYHSAKGVHFLTYNDF
                     HALQTVLETDYPQFVACLRELVSIFEGDMRSFMRSPKFHGHHEIELFGLRRGEALKHL
                     VPELEKLMEGIALKRHTQEEVAEQFQVIDGMFRASLERPELADEASDDFVETLYMHLT
                     GEVYYGQGDSVSLAFDDRRTALPGATFRGGRPDFHPGADDRTRVLLANVEQMMSQDEM
                     IEYANIYEVRSDSDAANDRAVGAGVTREIVFKTNRQPLCTSLIEKRLALKMPGYGSYM
                     LARVQAFKALGVGFGKYRLLMRLDSSAGRALNYFIRNRCPGEPLDEIPPRMFQRSGEF
                     GGSEAGEDPDVVLALGALLGNAAAQNLVLKKYLPEDDSCRFGEGKEVFEFGYDIASMR
                     EMPMRVMLCSVRGSLGWPDLAFTEENLDRLFRFYFDAYARVLYRYGLKHRDAVAMDDL
                     TERFFDGFECKTREMHWNYSVRREQFDAFDPQLRHKYAFTRKWRFALWSLDRQLRRLK
                     PLRVLFRERMAALAAEAEEAGG"
     gene            complement(4526..6454)
                     /locus_tag="PLZ74_07790"
     CDS             complement(4526..6454)
                     /locus_tag="PLZ74_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOR98261.1"
                     /translation="MTALPKRALVLAAGLGTRLRPLTQVWPKPLVPLWDVPLLEHVLR
                     LLESWGVEEVAVNLHWRPEAIRAYLETRRGGVRVTFSQEAELLGTGGALRPLRRFFGD
                     GPFWVVNADIAAAVDPQPFCEAFGGGHGLAAAWLEPKKGPRSVETDRRGRITCFRSPT
                     PGVPGTFTFCGLQLVSPRLYDFLPGRPFSTLVDAYTAAMAEGLFVDGVTVPGSYWDDA
                     GTVEALLRIHGEVKRLARRGKPGGALYRPEADRCDANATRFFCVGRDARVGRAVRGSD
                     SVVCGTARVGDASVLRRAVVAGGRVTGRLEDTVYVPLGGLDEASANRAVGALGWPVEE
                     TAVSCLGERGSNRSFWRLCRDARRAMLIRYSAERPENLRYAGHARLLADAGIPVPAVL
                     ADLPECGMLVLEDWGDDSLQRRMERRPGCAETWYAPVVKTLALWHRDVTRQVRECGAE
                     LEPAFDAELYAWERNLFETCLLVSRYGFDAIPAEAERELRGVADRLGRAPQVVVHRDF
                     QSSNILFRGKRFAFIDFQGMRRGAAAYDLASLLYDPYVILSVYVRNQMVAVYGKAYPE
                     HPEAVELFHDGAVQRLIQALGAFGRLASVGQRQFTRHIPRALESLLEAADLCGLDAVG
                     GLAEELIARERYRCRESG"
     gene            complement(6451..6936)
                     /locus_tag="PLZ74_07795"
     CDS             complement(6451..6936)
                     /locus_tag="PLZ74_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOR98262.1"
                     /translation="MGRGSGASAGALARNSVSGSGLDAVGGWRKNGVLVKGREAVDKL
                     ELRGLEVRCVIGDLPTERDREQVLTLDVTLACDLSAVCASDRVEDTVDYAVLAETIRD
                     ALRQARFRMIESAAARVAAVCLEAPRVSEVWVRIAKPGAVAGLREAAVELVRRRGDGA
                     S"
     gene            complement(6940..7755)
                     /locus_tag="PLZ74_07800"
     CDS             complement(6940..7755)
                     /locus_tag="PLZ74_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98263.1"
                     /translation="MLTVAGNALLGVLFTLLLGMTDTASWGWSVFWGVIVFVAGQALA
                     GYLIQRRVKSGMEAIQKILLDGQKRLQAKVNQWQNRPPGSLKQAQIEIEREQRVFVER
                     ALEASKGLERFKLWAPLMGRQINTLRLQFYWMLKEFKQVDALLPNALFLDPMMAAVKL
                     ARMHMKDETEGMERFFRKQTRRLRYGQGALLYGLYAWILVRQQDPDAAHKVLIEACEK
                     MENETLKKNREHLANNRIDLFSNAGLGDEWYALHLEQPKVKMQRQQRFSGRPF"
BASE COUNT         1540 a         2922 c         2135 g         1270 t
ORIGIN      
        1 gaaaagagtc aaccctgtaa atctgcaaaa aagcggggat tgggcgtacg gaatcaggat
       61 cggcggaccg cttaccccgt caccggcaga tagatcagga acgtgctgcc gcagccgggc
      121 tgggtctgca catcgatgaa cccgtggtgc tgttggacaa tcgcgtagac cgtcggcagc
      181 cccaggcccg cgtttttgcg atccgccttg gtggtgaaga aaggctcgaa aatacggcct
      241 gcggtctctg ccgtcatccc gcatccggtg tcccgcacct ccagcaccgc gaaacgtcct
      301 tgatgacgtt cgcacgccgg caaccccgcc atcaaacgcg tccgctcctc ctcggacagg
      361 gagatctcat acgtggagat cgtgacctgc ccgcggcccg cgaccgcctc caccgcattg
      421 atcgccagat gcacaatgag ctgctcgatc cggttggggt cgatcgtcac cttgccgtcc
      481 gcgacatccc cctgccggat caccagctcg acctcttcac cgatcgtctt gccgagcaga
      541 cgctccaccc cctccagaca cgtatccaac cgcatctggc gcaaccgcag cgcgtccgga
      601 atcgcgaacg ccaccagttc gcccatcaga ttcgagaccg tacccaccgt ccggatgacc
      661 tcgtccatgt tggcgaccac ccgtttcgga ctccgcggat cggccttcgt gagttccgca
      721 cggttttgaa tcacggtgag cgcgttcagc atcgtgtgag ccacccgata ggcgagctgg
      781 ccgatcgccg ccattttctg cgaacccagc atccgtcgct cggtctcggc caactgttcg
      841 accgttgtga tgtgacgctg ttccgagagg accaaagcgc tcagcagggc cattgaaacc
      901 ggcgtgatcc acagggctgc caatgccctt gccaccccga catcgaacac ggcccctctc
      961 ccctcgcaaa aggcgaacag ggccagcagg agattgctgc cgaggcccac cagcaggctt
     1021 aaggagagga ccagccgcgg cgtcaaggac agatcgtggc gtcgttgcca caggttggcg
     1081 agcgtcccca gaagaaccgg gaacaccagc gaccccacgc ccaggagcag gtgcggcgat
     1141 cccaacgcca accggtacgc aagcggcggc agcaggcata tcaacgcgac cggaggaccg
     1201 atcagcaacg cggccgtccc caacacgccg ccgcggcaat ccaccgtcag ccccgaatgg
     1261 gtgaccaccg gcgtgagcat gacgaccaca cccatgacgc cgtacagcaa cgcgaacgcc
     1321 cacggggcca gagtcctgcg ccgaagcagg gcgcatccgt tgaacacgct gcacactgcg
     1381 gcaaacccca gcagcacgaa cagattcgtg atcaatccgc gtatgaactc ggccagatta
     1441 attatcccct cctcgcgtac ccgctgccat cgctctcaag agcgcgacgc cacaatccgt
     1501 ttcaattcct caaccgcaac cgagtatccc ttgccgcaca tgtggcagat cacccgaacc
     1561 gactggtccg cacaaatcat ctcctccagt tcctttgtcg gcatgctgcc cagcatatcc
     1621 gaaacccgct ccgccgagca gggacagtgg aaacgcaacg gtttcggttc cgcaagctgg
     1681 acgtccggca cacccaccgt ctcgcagatc gtctgcagcg acgcgcccgc ctccagacac
     1741 tcctgaaccg tgccatcctc cacaaaccgg ccgatccgtt caaactcggc gcggtcaccg
     1801 tccggcaggc actcgatcag cacgccgcgc gcgcagtcga tgaacccgcc gtacgccaga
     1861 gccgtaagct gtacccgcac ccggcgctgc tgcgattggc ggtaataggt ttcgatcccc
     1921 tgcggcaccg aagccggccg cacctccacg cccgcgctcg acaggagaac cccgggcagc
     1981 gaacggataa cccgcacctc tgccgaatcg cccagcgcga ctgccgcatc cagatcctcg
     2041 caggcgtcaa gatcattcat gaccttcacc tgcgtatacc cgcgcaagct cccgtccgaa
     2101 cgcgcctcaa ccagcacccc gccgatcggt cccgtgacct gaaggcgcag ggtcaccgcc
     2161 tcctccggac gggtcagctc tgcccccagc aacgccaccc cggccagagc ctccgcctga
     2221 atcagcccgg ccgtgggccc gcagagatga ctgtgctcca gctcgcgcgc ggaatccgtg
     2281 acctccgcat gacgcaaaac gatgttcgcg gaagggcaaa aagccatgag cagccggtcg
     2341 gcatttttgg gcatgatcat ccgcccgcct cctccgcttc cgcggccagc gccgccatcc
     2401 gctcgcggaa caacacccgc aggggtttga gacgccgcaa ctggcggtcc agcgaccaga
     2461 gcgcgaaccg ccatttccgg gtgaacgcat acttatgccg caactgggga tcgaacgcat
     2521 cgaactgttc gcgccggacc gaatagttcc agtgcatctc gcgtgtctta cactcgaagc
     2581 cgtcaaaaaa ccgttccgtc agatcgtcca tcgcaacagc gtcgcgatgc ttgagcccgt
     2641 agcggtagag tacgcgggcg tacgcgtcaa aataaaaccg gaagagccgg tccagattct
     2701 cctccgtgaa ggcaagatcg ggccagccca aagaaccccg gaccgaacac agcatcaccc
     2761 gcatgggcat ctcccgcatg ctggcgatgt cgtagccgaa ctcgaacacc tccttgccct
     2821 cgccgaaacg gcaactgtcg tcctcgggca gatacttctt cagcaccaga ttctgcgcgg
     2881 cggcgttgcc cagcaacgcg cccagcgcca acaccacatc cggatcctcg cccgcctcac
     2941 tgccgccgaa ttcgcccgaa cgctggaaca tgcgcggcgg gatctcatcc agcggctcgc
     3001 ccggacaacg gttgcggata aaataattca gggcacgtcc ggcggagctg tccagccgca
     3061 tcagcagccg gtacttgccg aaccccacac ccagcgcctt gaaggcctgc acgcgcgcca
     3121 acatgtaact gccgtacccc ggcatcttca gcgcgagccg cttctcgatc aggctcgtgc
     3181 aaagcggctg gcggttggtt ttgaaaacga tctcgcgcgt gacgcccgcg ccgacagcgc
     3241 ggtcgttggc ggcatcgctg tccgaacgga cctcgtaaat gttggcatac tcgatcatct
     3301 cgtcctgact catcatctgc tcgacgttcg caagcagcac cctcgtgcgg tcatccgcgc
     3361 cgggatggaa gtccggtcgg ccgccgcgga acgtcgcgcc gggcagcgcg gtgcggcgat
     3421 cgtcaaacgc cagcgagacg ctgtccccct gaccgtaata cacctcgccc gtgagatgca
     3481 tgtacagcgt ctccacaaaa tcgtcgctcg cctcatccgc cagttcgggc cgctccagcg
     3541 aagcccggaa catcccgtcg atcacctgaa actgttccgc cacctcctcc tgcgtgtgcc
     3601 gcttcagcgc gataccctcc atgagcttct ccagttccgg cacgagatgc ttcagcgcct
     3661 ccccgcgccg caggccgaag agttcgatct catgatgccc atggaacttg ggcgaccgca
     3721 tgaatgaccg catgtcaccc tcgaaaatcg aaaccagctc gcgcagacag gcgacaaact
     3781 gcgggtaatc ggtttccagc accgtctgga gcgcgtggaa atcgttgtac gtcagaaaat
     3841 gcacgccctt ggcgctgtgg taatacttca gacaggtgtt gatgcgcgtg cggctcgcgc
     3901 gcagggccgc atccatctcc cgccgcgtcc aggcgagggg cttgatccgc cactcctgcc
     3961 ccaaccggcg gtaatgatcc agcgtcatgt ccgtgcgctc ggccatgatg acctcgtcca
     4021 gcgcaaaccc cacgttgcgc aaccattcgt cagccttgat cagcagccgg gtcggcacgt
     4081 cacgctgcga gatttcgagg ttcccgttca ccacgttggc gcacagcacc tcttcccgca
     4141 tcacctggct gcgccgggca gcgggcacgc gcccgagatt ccagaagtgg ccggagagca
     4201 gcgggatcgc ggcgaccgtg tggttgccgc tggtcagcgt cagaatgtgc ccgcccgaac
     4261 gacggatgag ttcgctcttc cggacgatca ggaccgggct gtgctcctcc agattcttcc
     4321 acacgtcctt ttcgaagatg aacgtgtcaa ccccccagtc gtatccataa aaaacgcgat
     4381 cgcggcccat ggaccgcacc gtgaccggct ccgtcttctg ccgggcttcg cgcctcacgc
     4441 gggcgataaa ctggtgaacc gtcttcttca tccgctgccg tacacctttg tgccgccggt
     4501 caacgcattc caccggcagc accccttacc ccgactcccg gcaccggtag cgctcgcggg
     4561 cgatcagctc ttccgccaac cctcccaccg catccagacc gcacaggtcg gccgcctcaa
     4621 gcagggattc cagcgcccgc gggatgtgcc gggtgaactg tctctgcccc acggaagcca
     4681 gacggccgaa ggcgccaagc gcctgaatca accgctgcac cgctccgtcg tgaaacagct
     4741 cgaccgcctc cgggtgctcc ggatatgcct tgccgtatac cgcaaccatc tgattgcgca
     4801 cgtaaacact tagtataaca taaggatcat agagaagaga agccagatca taggccgccg
     4861 caccccggcg catcccctga aaatcaatga acgcgaaccg tttgccgcgg aagaggatgt
     4921 tgctggactg gaaatcgcgg tgcacaacca cctggggggc gcgtcccaac cggtccgcca
     4981 cccctctcaa ctcgcgctcc gcctccgcag gaatcgcgtc gaatccgtag cggctgacga
     5041 gcagacaggt ctcgaacagg ttccgttccc acgcatacag ttccgcatcg aacgccggct
     5101 ccagttccgc gccgcactcc cgcacctgcc gcgtgacgtc ccggtgccac agcgcgagcg
     5161 tcttcaccac cggggcgtac catgtctccg cacacccggg ccgccgctcc atgcggcgct
     5221 gcaagctgtc gtctccccag tcctccagca ccagcatccc gcactccggc aggtcggcga
     5281 gcaccgcggg caccggaatc ccggcatcgg ccagcagccg cgcatgcccg gcgtaccgaa
     5341 ggttttcggg acgttccgcc gaatagcgga tcagcatggc gcgccgcgcg tcccggcaca
     5401 gccgccaaaa gctccggttg gagccgcgct cccccaagca ggagaccgcc gtctcctcca
     5461 ccggccaacc gagcgccccg accgcccggt ttgcggacgc ttcgtccagc ccgccgagcg
     5521 gcacgtatac cgtgtcttcc agacgtcccg tcacccgccc gccagcgacc accgcacgac
     5581 gcagcacact ggcgtcaccg acccgcgccg tgccgcacac cacactgtcc gagcccctca
     5641 ccgcccgtcc gacccgcgcg tcccggccga cacagaagaa ccgcgtggcg tttgcgtcgc
     5701 aacggtccgc ctcgggccgg tacagtgccc ccccgggttt gccacgccgc gccagccgct
     5761 tgacctcgcc gtggatccgc aacaaagcct ccaccgttcc ggcatcgtcc cagtaacttc
     5821 cgggcacggt cacgccatcc acaaaaagcc cctcggccat cgctgccgta tacgcgtcga
     5881 cgagcgtgct gaagggccgg ccgggcagaa aatcgtagag ccgcggcgaa accaactgta
     5941 acccgcaaaa agtgaaggtg cccggcacgc cgggcgtggg gctccgaaaa caggtgatcc
     6001 gccctcggcg gtcggtctcc accgagcgcg gacccttttt cggttcgagc caggccgcgg
     6061 ccaaaccatg acccccaccg aacgcctcgc aaaacggttg cggatccacg gcggcggcga
     6121 tgtccgcgtt gaccacccag aacggcccat ccccgaaaaa ccggcgcaac ggacgcaatg
     6181 ccccgcccgt gcccagcagt tccgcctcct gcgagaacgt gacccgcacc ccgccgcgcc
     6241 gcgtctccag atatgctcgg atcgcttcgg ggcgccaatg caggttcacg gcgacctcct
     6301 ccacccccca agactccagc agccgcaaca catgctccaa cagcggcacg tcccacagcg
     6361 gcaccagcgg cttaggccaa acctgcgtca gcggcctgag ccgcgtgccc aatcccgcgg
     6421 ccagcaccag cgcccgtttc ggcagcgcgg tcatgacgcg ccgtcccccc ggcgccggac
     6481 gagttcgacc gccgcctcgc gcaaccccgc caccgcgccg ggcttggcaa tccggaccca
     6541 gacctccgac acgcgcggtg cctcaagaca gactgcggcg accctcgcgg cggcgctttc
     6601 gatcatacgg aaccgggcct gccgcaacgc gtcgcggatc gtctcagcca gcaccgcgta
     6661 atccaccgtg tcctccaccc ggtcactcgc gcaaaccgcg ctcagatcac aggccagcgt
     6721 cacatccagc gttaacacct gctcgcggtc acgctccgtc ggcaaatcgc cgatcacgca
     6781 ccgcacctcc aacccgcgca actcaagctt gtccactgct tcccgccctt tgaccagcac
     6841 gccgttcttc cgccaacctc ccaccgcatc cagcccggaa ccggaaaccg agttgcgggc
     6901 aagggcgccc gcgctcgctc ccgaccctct gcccatccct cagaatggcc gaccgctgaa
     6961 gcgctgctgc cgctgcatct tgaccttcgg ctgttccaga tgcagcgcgt accactcgtc
     7021 acccaagccc gcgttggaaa aaaggtcgat ccggttgttg gcgagatgct cacggttctt
     7081 tttgagcgtc tcgttctcca ttttttcgca ggcctcgatc aagaccttgt gcgcggcatc
     7141 cggatcctgt tgccgcacca gaatccacgc gtacaaccca tacaaaaggg cgccctggcc
     7201 gtaacgcagc cgtcgcgtct gtttccggaa gaaacgctcc atcccctcgg tctcgtcctt
     7261 catgtgcatg cgcgcgagct tgaccgccgc catcatggga tccagaaaaa gtgcgttcgg
     7321 caacagcgcg tccacctgct tgaactcctt gagcatccaa taaaactgca accgcagggt
     7381 gttgatctgc ctccccatca gcggtgccca cagcttgaac cgctctaacc ctttcgaggc
     7441 ctccagcgcg cgttcgacaa agacccgctg ttcgcgctcg atctcgatct gcgcctgttt
     7501 caggctcccc ggcggacggt tctgccattg gttgaccttg gcttgcagac gcttctggcc
     7561 gtccagcagg atcttctgga tcgcctccat cccggatttg acgcgacgct ggatgagata
     7621 ccccgccagc gcctgccccg cgacgaacac gatcacgccc caaaaaacgc tccatcccca
     7681 tgacgccgta tccgtcatcc ccagtagcaa cgtaaacaaa acacccaaca acgcgtttcc
     7741 tgccacagtc agcatgggca cccccttctg caatgaaaaa gaagagttta ccctgatgcc
     7801 cggatacggg caactgtaaa ccgggacacg gataggatcc gcggtatgtc atagtcatgg
     7861 acaccgt
//
LOCUS       DAOSRB010000132         7840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8732_length_7840_cov_6.566089, whole genome shotgun sequence.
ACCESSION   DAOSRB010000132 DAOSRB010000000
VERSION     DAOSRB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8732_length_7840_cov_6.566089"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLZ74_07805"
     CDS             complement(<1..555)
                     /locus_tag="PLZ74_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98264.1"
                     /translation="MVKKTSKKKRIAVGTLLVLGAAAWFWFGKRDKTSEAVPVFAVQQ
                     GPLTIAVTSGGSIQSRDKIVIASELEGNNTVIWVIDEGVSVQPGDLLLEFDSSDLIEK
                     RNEQEIVVANAEANLIITQEKLGITEGDCEAALLDREVDMMLAKMALEKYQDGDYPQQ
                     LRQSEADVALADEELKRADERLEWS"
     gene            complement(548..2281)
                     /locus_tag="PLZ74_07810"
     CDS             complement(548..2281)
                     /locus_tag="PLZ74_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR98265.1"
                     /translation="MRRAGSYLRKVRVGHGLTVFGVAGVCLLAPIACMTPGRAVLETD
                     ETGTRLATAYWQQQTGSTNTFDMSRPADTLTLRIALLAAARGEQNVIFPAIPNLGELN
                     VSNEVIVLTLPHALCLAARNDRSYQKLKEAVFDAALDLDYQQYRFGTTFSGMMFGLLS
                     GDPGIEKASGGVSAGAARQTEAGLAVAGRLALNVVSLLRDDWRSMGLTGDLSMTLPLL
                     RGAGREIVREPLTQAERNLTVAIWNFERYRQTYAYSVASAFYNVLEYGQRLKNAIDNE
                     SRLQENSRRAEMWFEAGRMERIQVDQARSDLLNAGESVINTRKNVEEKLDAFKIQIGL
                     PPEAKIVLDERELDELEKEMERIAERSPNAVEAFPEEDEACRIALAERHDLLITRSAL
                     EDVARSVKIAADALRADVTLQGGGSLDRLRETGRSGFKGGEALEGGLSVSLPWDRRRE
                     RNAFKKRLLELEQAKRALEAMEDTVKQAVRSGFRNLIAARASYENQQETMRVARLRLE
                     SNNLFLLSGRSSMRDILEAESAMLTARNALCSAVIQWRLSELALRRDMGVFGIGEDGM
                     WRETVDRGNHG"
     gene            complement(2371..3468)
                     /locus_tag="PLZ74_07815"
     CDS             complement(2371..3468)
                     /locus_tag="PLZ74_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOR98266.1"
                     /translation="MSGGQCVGWESRVLLEAVTELSHAFGSAAYVKGGGGNTSCKQAD
                     TLWVKPSGTTLAALTPDRFVALDRARLACLYRETPPDGAAAREAWVKERMDAAVRPET
                     PGRASVEAPLHDTLAARFVVHTHPEACNGMTCAVGGEAAAARLFPEALWIPYVDPGYT
                     LCREVRCAVQDYAREHGAEPALIFLQNHGVVVSGDTVEAVRAVYESLMERLSREYAEA
                     HLDGGAHVGPEPAEHEVAGWRQTLSETFGSEAAAVAVSGMLDVGAGPISPDHVVYAKS
                     YPFEGRVTPENLRAFQSVRGYPPRVVVTGSAVLGVGPRESVARVALDLARDGAEVKRL
                     ARAFGGLRYLSDAARQFIENWEVESYRAQQM"
     gene            complement(3465..4727)
                     /locus_tag="PLZ74_07820"
     CDS             complement(3465..4727)
                     /locus_tag="PLZ74_07820"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOR98267.1"
                     /translation="MSKKNSGTAYRIACERYAEMGVNVEAALGKLAQIPISLHCWQGD
                     DVRGFENPEGDLTGGIQTTGNYPGRARTPQELRGDIAFVLSLVGGSHRVNVHACYAEF
                     EKGKKVDRDALQPEHFKNWIAWAKQNRLGMDFNPTLFSHPRFDGFSLASRDKRTRNFW
                     IRHTQATRRIAAAIGKALGTPSVNNVWIPDGYKDIPADRATPRKILNDALDECFAEKL
                     NPRHMLDAVESKLFGIGAESYTVGSHEFYMGYAVKNQKLLTLDAGHFHPTETISDKLS
                     ACLPFVPGILLHVSRGVRWDSDHVVLVGDDLIAIAQELVRLDQFRRIHIGLDYFDASI
                     NRIAAWVLGMRAMQKALLYALLEPFETMREAEREGDLTARLVWTEAAKTMPFGAVWEK
                     FCLEEDVPPDPAWLNEVKRYEAQVLAKR"
     gene            complement(4927..5643)
                     /locus_tag="PLZ74_07825"
     CDS             complement(4927..5643)
                     /locus_tag="PLZ74_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98268.1"
                     /translation="MERLKTQLETLILELEDSTEIYERLENLISVYPFNEYEYMVSTL
                     MGRGKLSLDDYYALRDEYIGRNMYLYIFEISAPRGFGETWAQGHLKELVPELQKPSKK
                     KDPAYSGQYDFCLDDRIAIEVKASRAVDFELQAPLYVKALASDSTRPFDMNFQQVKPG
                     CCDVFVWVGVWRDKIRYWVLASREMESNRYYSKGQHRGNVGEGQLHLRHDNIAEFADY
                     EVIGRDLLQAIKAAYDRQHA"
     gene            complement(5643..6608)
                     /locus_tag="PLZ74_07830"
     CDS             complement(5643..6608)
                     /locus_tag="PLZ74_07830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOR98269.1"
                     /translation="MKNCTLARLDEDEKARQSVLPHCRLAPGEIWIDPVRGHRIGCVD
                     ATDARQIAELMDGQPASLAVHDPPYNLVAFGKRSVGDFISWCARWIETTSASLCADAS
                     LYVWLGADQNEGFQPLPDFMLMMRGFKEFSSRSLITMRNQRGYGTQKNWMAVRQECLY
                     YTKGSPSFAVQYTDIPKILRGYYKEINGKKTENMERSKSENIRPGNVWVDIQQVFYRM
                     EENVNGCYAQKPIKAIERLVAASSREGDTIIDFFGHSGTTLIVAEQMKRRCMTCDVDP
                     IYCEIMIRRLERLRTYGKTGWQNGNPFEKELHGVVESEQPVLELW"
     gene            complement(6795..7295)
                     /locus_tag="PLZ74_07835"
     CDS             complement(6795..7295)
                     /locus_tag="PLZ74_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOR98270.1"
                     /translation="MKKLAIVILGAPNDAEGNLSSIAIERCQRALTEYAANPDAKMLP
                     TGGWGDHFNTTDKSHGYYTRQYLMAHGVPERNILECAESANTIQDAQLVRPIVRRHEI
                     DALIVVTSDFHIPRARFLFEREFPDLRLSFSAAQTHLPEEELGRLKEHEAKALARLKQ
                     SNAIGN"
     gene            complement(7461..>7840)
                     /locus_tag="PLZ74_07840"
     CDS             complement(7461..>7840)
                     /locus_tag="PLZ74_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98271.1"
                     /translation="LPIAVRTNIVLNVRGIIRGRDRLNDTVRHAVRTPIRLWKTAEMI
                     VLGHILFGLGSLACFVGEIKMLTLAYRRGPGWFLSCIFLAPICWLALLAVDCKLAIKP
                     FVLACAGLIAIGIGGSLAGIEFG"
BASE COUNT         1558 a         2603 c         2018 g         1661 t
ORIGIN      
        1 cgaccactcc aaccgctcat ccgcgcgctt caactcctcg tccgccagcg ccacatccgc
       61 ctccgactga cgcagttgct gcggataatc gccgtcctga tatttttcca gcgccatttt
      121 ggccagcatc atatccactt cccggtccag cagcgccgct tcgcagtccc cctcggtgat
      181 gccgagtttc tcctgggtga tgatcaggtt cgcctctgcg ttggccacca cgatctcctg
      241 ctcgttacgt ttttcgatga ggtcgctgga atcgaactcc agcaacaaat cgcccggctg
      301 cacgctgacc ccctcgtcga tcacccagat caccgtgttg ttgccctcta attcgctggc
      361 gatcacgatc ttgtcgcggc tctggatcga tccgcccgag gtgaccgcga tcgtcagagg
      421 cccctgctgc acggcaaaaa ccggcacggc ttcgctggtt ttgtcccgtt tgccgaacca
      481 gaaccacgcc gccgcaccga gcaccagaag cgtgccgacc gcgattcttt ttttcttgga
      541 tgtcttctta accatgattg cctcggtcca ccgtttcgcg ccacatcccg tcttccccga
      601 tcccgaacac gcccatatcc cggcgcaggg ccaattcgct gagccgccac tggatcacgg
      661 cggagcataa cgcattgcgc gccgtgagca tcgcgctctc cgcctccaga atgtcacgca
      721 tcgaggaacg tccggaaagc agaaagagat tgttgctttc caaccgcaga cgggccacgc
      781 gcatggtctc ctgctggttc tcatacgacg cgcgggccgc gatcagattc cggaatccgc
      841 tgcggaccgc ctgtttcacc gtgtcctcca tagcctccaa cgcccgtttg gcctgttcca
      901 gctcgagcag acgctttttg aaagcgttgc gttcgcgccg ccggtcccac ggcaacgaca
      961 ccgaaagccc cccctcaagc gcctcgccgc ccttaaaccc gctcctcccg gtttcacgca
     1021 ggcgatccag actgcccccc ccctgaagcg tcacatcggc ccgcagggca tccgccgcga
     1081 tcttcacgct acgcgccaca tcctcgagcg cgctccgcgt gatgagcaag tcatgccgct
     1141 ccgccagcgc gatgcggcac gcctcgtcct cttcgggaaa cgcttcgacc gcattcggac
     1201 tgcgttcagc tatccgctcc atctccttct ccaactcgtc cagttcgcgc tcatccaaaa
     1261 cgatcttcgc ctccggtggc aaaccgatct ggattttgaa agcgtccagt ttctcttcca
     1321 cattcttacg ggtgttgatg acgctctcgc ccgcgttcag caggtcggaa cgcgcctggt
     1381 cgacctggat gcgctccatg cggcccgcct cgaaccacat ttcagcacgg cggctgtttt
     1441 cttgcaggcg gctctcattg tcgatcgcgt ttttcagacg ctgcccgtat tccaacacgt
     1501 tataaaacgc gctggccacc gaatacgcgt acgtctggcg gtatcgttcg aagttccaga
     1561 tcgcgaccgt gaggttccgc tccgcctggg tcagcggttc gcgcacgatc tcgcgcccgg
     1621 cgccgcgcag aagcggaagg gtcatggaaa gatcgccggt cagtcccatc gaacgccaat
     1681 cgtcacgcaa caggctgacc acattcaggg ccaaccgccc ggcaaccgcc agcccggcct
     1741 cggtttgtct ggccgcgcca gcggaaaccc cgccgctggc tttttcgatc cccggatctc
     1801 cggagagcag accgaacatc ataccgctga aggtcgtgcc gaaccggtac tgctggtaat
     1861 ccagatccag cgccgcgtca aacaccgcct ccttcagctt ctggtacgaa cggtcattcc
     1921 ttgccgccag acacagggca tgcggcaacg tgagcacaat gacttcgttg gagacgttca
     1981 gttcgcccaa gttcgggatg gcggggaaga tgacgttctg ctccccgcgc gccgccgcga
     2041 gcaacgcgat gcgcaacgtc agcgtatccg ccggacggct catgtcgaac gtattggtcg
     2101 atccggtctg ctgctgccag taggcggtcg ccaaccgggt gccggtctca tccgtctcca
     2161 gcaccgcccg ccccggcgtc atgcaggcga tcggagcgag cagacagact cccgccactc
     2221 caaacacggt caacccgtgt ccgacacgca cctttcgcag gtatgatccc gcacgcctca
     2281 tttgcccccc aaaaaaacct ttcgtccgca catccctacg gcttccccaa gcccccggcc
     2341 cgcgcataca ccttccccaa cacgttccgc tcacatctgt tgcgcccgat aggactcaac
     2401 ctcccaattc tcgatgaatt ggcgcgccgc gtcactcaga taccgcaacc cgccgaacgc
     2461 gcgcgccaac cgcttgacct ccgcaccgtc acgggccaga tccagcgcca cccgggccac
     2521 gctttcgcgc ggcccgacgc ccagcaccgc cgaacccgtg accacgacgc gcggcggata
     2581 gccccgcacc gactggaacg cgcggagatt ctccggtgtg acgcgccctt caaacggata
     2641 cgacttcgca tacacgacat ggtccggaga aatcggtccg gcccccacat ccagcatgcc
     2701 ggatacggcg accgccgccg cctcgcttcc gaaggtctct gacagcgttt gccgccaacc
     2761 ggccacctca tgttccgcag gctccggtcc aacgtgcgcc ccgccgtcca gatgcgcctc
     2821 cgcatactcg cgggacaacc gttccatcag gctctcgtac accgcacgca ccgcctccac
     2881 cgtgtcccct gaaacgacca caccgtgatt ctggagaaag atcagcgcag gctccgcacc
     2941 gtgttcccgc gcatagtcct gaaccgcgca ccgcacctcc cggcacaacg tgtaaccggg
     3001 gtccacatac ggaatccaga gcgcctcggg aaagagacgg gccgcagccg cttcaccgcc
     3061 gaccgcacaa gtcatgccgt tacaggcctc cggatgcgta tgcaccacga aacgggccgc
     3121 gagcgtgtcg tgcagcggcg cctccactga ggcgcgtccg ggcgtctctg gccgcaccgc
     3181 cgcatccatc cgctccttga cccaagcctc gcgcgcggcc gctccgtccg gcggcgtctc
     3241 ccggtagagg cacgccagcc gggcgcggtc cagcgcgaca aaccggtcgg gggtcagcgc
     3301 ggcgagcgtg gtaccggagg gcttcaccca gagggtgtcc gcctgcttgc aggaggtatt
     3361 gcccccgccg cctttgacat acgctgcgga tccgaacgca tgcgacagtt cggtaaccgc
     3421 ttcgagtaac actctactct cccaccccac gcactgccct ccgctcaccg tttcgccagc
     3481 acctgcgcct cataccgttt gacctcattg agccaagccg gatcaggcgg cacgtcctcc
     3541 tccaggcaga acttttccca gaccgcgccg aacggcatcg tcttcgcggc ttcagtccac
     3601 accaggcgcg cggtcaggtc gccttcccgc tccgcctcgc gcatggtctc gaacggctcc
     3661 agcaaagcgt acagcaacgc cttctgcatg gcgcgcatgc ccagcaccca cgccgcgatc
     3721 cggttgatgc tcgcgtcaaa gtaatccagc ccgatgtgta tgcgccggaa ctggtccagc
     3781 cgcaccaact cctgcgcgat cgcgatcaga tcgtccccca ccaacaccac atggtcgctg
     3841 tcccaccgca ccccccggct cacatgcagc agaatacccg gaacaaacgg caggcaggcg
     3901 gagagtttgt ccgagatcgt ttcagtcgga tggaagtgcc ccgcatccaa ggtcagcaac
     3961 ttctgatttt tcacggcgta ccccatgtag aactcgtggg aaccgaccgt ataactttcc
     4021 gcaccgatgc cgaagagttt ggattcgacc gcatccagca tgtgtctggg gttcaacttc
     4081 tccgcgaaac actcgtccag cgcgtcgttc aggatcttgc gcggggttgc gcggtcagcc
     4141 gggatgtcct tgtagccgtc cggtatccaa acgttgttga cactgggcgt gcccagtgct
     4201 ttgccgatag ccgcggcaat ccgccgcgtg gcctgagtgt gccggatcca gaaattccgg
     4261 gtgcgtttgt cgcggctggc cagagaaaat ccgtcaaacc ggggatgcga gaagagggtc
     4321 gggttgaaat ccatgcccag cctgttctgc ttggcccagg cgatccagtt tttgaagtgt
     4381 tccggctgca acgcgtcgcg gtcgactttc ttgcccttct cgaactccgc gtaacaggcg
     4441 tgcacgttca cgcggtgcga gccgccgacc agcgaaagca cgaacgcaat gtcgccgcgc
     4501 aactcctgcg gggtgcgcgc gcgccccgga taattaccgg tggtctggat cccgcccgtg
     4561 agatccccct cgggattctc gaagccacgc acgtcgtcgc cctgccagca gtgcaacgaa
     4621 atcgggattt gagccaactt cccgagagcc gcctccacgt tgacccccat ctccgcatag
     4681 cgttcacacg cgattcgata cgcagtcccg ctgtttttct tgctcatgag atcgcccctt
     4741 ttcctgaaat gggataacag ggggaagttt accggattcc acccgcaaca tcaattccaa
     4801 ccgtaacgcg gacttgcccc atttttttca agccgagccg gataatggct gttccggcta
     4861 catttcggcg gtttccggca catgctgttt gcggtggcac gtccgctgaa tttgtcttgc
     4921 tggacatcat gcatgttgcc ggtcatacgc agctttgatc gcctgaagaa ggtctctgcc
     4981 gatcacctca tagtcagcga attccgcaat gttatcgtgt cgcaggtgca gttgcccttc
     5041 accaacgtta ccacgatgtt gtcccttgga gtaataacgg tttgactcca tttctcggga
     5101 cgcgagcacc cagtaccgaa tcttgtcacg ccacacgcca acccacacga agacatcaca
     5161 acaaccgggt ttcacctgct ggaagttcat gtcaaatggg cgagtggaat ccgacgcgag
     5221 agccttcaca tagagagggg cttgaagttc gaagtccacg gcacgcgaag ctttaacctc
     5281 gatggcgatc ctgtcatcga gacagaagtc gtactgtccg gaataggccg gatctttttt
     5341 ctttgatggc ttctgaagtt cgggaacaag ttccttgagg tgcccttgtg cccatgtctc
     5401 gccgaagcct ctcggagcgc tgatttcaaa aatgtacagg tacatattcc gtccaatgta
     5461 ttcatctcga agggcgtagt agtcatcaag ggaaagcttg cctcgaccca tcagtgtgga
     5521 gaccatgtat tcgtattcat tgaacgggta gacagagatc agattttcaa gtcgctcata
     5581 gatttccgtc gagtcttcga gttctaagat gagcgtctca agttgcgttt tgagtctttc
     5641 cattaccaca gctccaatac gggttgttcg ctttcgacaa caccgtgaag ctctttctca
     5701 aatgggttgc cgttctgcca tccggtcttt ccatacgtgc gcagacgctc cagacgccga
     5761 atcatgattt cacaataaat cgggtcaaca tcacaggtca tgcacctgcg cttcatctgc
     5821 tccgctacaa taagggtggt gccagagtgt ccgaagaagt cgattatagt atcaccttcg
     5881 cgtgaacttg ctgcgacaag gcgctcaatc gccttgatgg gcttctgtgc gtagcacccg
     5941 ttcacattct cttccatgcg gtagaatact tgttgaatat ccacccagac attccccgga
     6001 cggatattct cggacttgct ccgttccata ttctccgtct tcttaccgtt gatctctttg
     6061 tagtacccac gaagaatttt gggaatgtcc gtgtattgga cagcaaacga ggggctcccc
     6121 ttcgtatagt agagacactc ttgtcgaacc gccatccagt tcttctgcgt cccatatccg
     6181 cgctgattgc gcatggtgat caaggatcgc gaggaaaact ctttgaaacc gcgcatcatc
     6241 aacatgaagt ccggtaacgg ctgaaaccct tcattctggt cggcaccaag ccagacgtac
     6301 agactggcat cagcacagag ggaagcgctt gtggtctcaa tccatcgagc acaccagctg
     6361 atgaaatcgc cgacagaacg tttcccgaac gcgacgagat tatatggggg atcatggacg
     6421 gccaaagaag ccggttgtcc atccatcaac tcggcgatct gtcgggcatc agttgcgtca
     6481 acgcatccga tgcgatggcc acgtaccggg tctatccata tttcgccggg agccagtcgg
     6541 caatgaggga gtacgctctg tcgggccttc tcgtcttcgt cgagtctggc tagtgtgcag
     6601 ttcttcatag tctcattttc tcaaacctaa cagcgttacc gggcctgcct cgcgaaagca
     6661 gacaaacccc atatacgttc aggataatat tcaccctgac ggctatgggc aagcgcatat
     6721 tttgtgttga ctattatccc tcgcggtttt gctttcttca cgaacgccgg gcacattgcg
     6781 cgacagagca cgggttagtt cccaatggcg ttgctctgct tcagtctggc caaggccttc
     6841 gcctcgtgtt cctttagccg tccgagttcc tcctcgggca gatgcgtctg ggccgccgag
     6901 aatgatagcc ggaggtccgg aaactcacgc tcaaacagga accttgcccg tgggatatgg
     6961 aaatcagacg tgacaacgat taacgcatcg atctcgtgac gcctgacaat cggcctgaca
     7021 agttgcgcgt cttggattgt gttggcgctt tcagcacact ccagaatgtt cctctcggga
     7081 actccgtgcg ccatcaggta ctgccgagtg taatagccgt gagatttgtc cgttgtgtta
     7141 aagtggtctc cccaccctcc tgtcggcagc atcttggcgt cgggattcgc cgcatactca
     7201 gtcaacgcgc gctgacatcg ctcgatcgcg atagacgaaa gatttccctc ggcgtcgttg
     7261 ggcgcaccca gaatgactat ggccagtttc ttcatcactc tccacctccg cacacagttt
     7321 caaacacttc tctgtgtcag tcaattgagt acaggcgggc gtcacgcgcc gcgcggattt
     7381 caccttcgat ttcggcaggc gtcatccccg ccacgtcgta gctccgttca gtgtcgccgc
     7441 tagttaggct tcggttgaag tcatccgaat tctatccctg ccaatgaacc gccaattcca
     7501 atggcaatta gcccagcaca tgccagcaca aacggtttta tagcgagttt acagtccact
     7561 gcaagcaatg ccagccaaca tatcggggca agaaagatgc aactcaagaa ccagcccgga
     7621 ccgcgcctgt aggcaagagt taacatctta atctcaccaa caaaacacgc caaactaccg
     7681 agaccgaaaa gaatgtgtcc caaaactatc atttctgcgg tcttccaaag ccgtattggt
     7741 gttcttaccg cgtgccggac agtgtcgttg agcctgtctc gcccgcgaat aattccacgc
     7801 acgttcaaaa caatattcgt tctgacggct ataggcaagc
//
LOCUS       DAOSRB010000133         7759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_8846_length_7759_cov_9.442757, whole genome shotgun sequence.
ACCESSION   DAOSRB010000133 DAOSRB010000000
VERSION     DAOSRB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8846_length_7759_cov_9.442757"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..1492
                     /locus_tag="PLZ74_07845"
     CDS             281..1492
                     /locus_tag="PLZ74_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOR98272.1"
                     /translation="MRKILLVCAMATARLVWGQVSDTAKDDAAADRDKALRAQLPGLF
                     RDAAEQYRGMLKQLEGTQDAFPRRWEKGSLVTIGPKEWTSGFFPGSLWLLYEATREET
                     WKREAMRYTAMLESVRHYTGNHDIGFMLYCSFGNGLRLANPDGYREVLLDGAAALCKR
                     YVPSLGMIRSWDSYKNPVIIDNMMNLELLMWAAKQSGEPRFREIAVSHADQTNRRHFR
                     PDGSAYHIVDYNPATGKIFGYYAGQGASADGPWARGQSWGLYGFTLMFRETGTPAYLT
                     RAVSLADFLIGHRNLPADAIPYWDYEAAGIPNEPRDASAAAIMASALLELATFAEAPK
                     AVRYRDTAVRQLLALSSPAYRAPVGENGHFLLMHGVGHLPGNKEIDVPLNYADYYYLE
                     ALLRFRRLQNN"
     gene            1527..1919
                     /locus_tag="PLZ74_07850"
     CDS             1527..1919
                     /locus_tag="PLZ74_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOR98273.1"
                     /translation="MMLSEFVKSADWKNEKHVPVIECADTVQAGKPLAITISVGKETA
                     HPNTTNHHIVWIALHYVPAGAKTSIEIARCEFSAHAAGVEGPDKGPARTCSTVSISAE
                     FAVSGTLYATAYCNIHGLWASEQPITVA"
     gene            1943..5827
                     /locus_tag="PLZ74_07855"
     CDS             1943..5827
                     /locus_tag="PLZ74_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98274.1"
                     /translation="MRSRLPLVLGGVGLALLVRAAPLASDLLLWLPCDEGGGAFTEDR
                     SPNRLEAELSGVQWVTGSFGTALRFNGTNALAELPPVPGLNGAERFTLALWAVWEDPA
                     PRRYPCLLSSHTWSPGGMLLFVSDQTCSFRLGRPGHRAGTPGHAWNESSVNLLSDLPQ
                     RRWTHLCVTFDRPHLTSYVGGKPVARTRWDHAVEADALRLGGWSGAVCHNGLIDDLRI
                     YGRALTPSEITALANPSDRADAAWKPATERERAPEPPFARFRTRHAELTLNARGRVTS
                     LRNRASGRELLVRPTPLVSARLRNGQRITARSVTRQGDALTFGFATHAGSAVVAIDAH
                     REFFNFTLRTHSLTDATELVFCELPVRPTRYRGGMANLLSDDEDGVCLRGYDLPVEMR
                     IAGDVLRVSAAVPPGPSGWRAGLAAGPRDTLTTSLRAMARHAGVPMSENSGPWSLEAE
                     ATRGSYLFANLAHAATDDWIEVARRGGFSTLHLHGWWQTLGHYAVRTQYYPRGLDDMR
                     DTVERIHAAGLRAGIHTLTACIDTRDAWITPEASPHLIPFDTYTLARSVSPTDTVLYV
                     NEPPSARHDVVFTYSGNGNAIRIGSEIIQYAAITREPPYAFRDCTRGAFKTRPAAHAA
                     GAQADYLQQRYNAFYPQPDSPLADELADRIAHVFNTCKLDQLYFDGSEGMMSRYGIDA
                     MRHKIMRRLEGNPLIEASCHGAHNWWFHSRLGAWDHPVWAPKRFHDDHVAVCARYRAS
                     DLLAPQMGWWAPRMATPFARGHHLDEMEYFAGKNLGLDAAMSIQGVNVNQAILPYHLE
                     RQFTLLGWYEHLRLARYFDTQTVARLIVPGAEFRLRQTRDGIWQFTPVTMRTHRVTGP
                     DDGSAAWRVTHPHAQQPLCLRVEALYDAAPAATPPAGKSLVGTNDFAAFAVSTASAAV
                     RLNVSEVSEETRGGNRHLRLRAENRGTTSTGAWARAVLTFPAPYRNLSGTGAFGMWVK
                     GDGSGALLNLQVGCPREFMGGLSDHYVTLDFTGWRYVTLLTRERDTARMDEHVWPYRK
                     AGSHALFRTNLDMAHLSQVSLYLNALPAGAHVEVTVSPVEALPVRSVDMKRPVVTVNG
                     QGLTVPFTLASGEFAELEPDGLFTHYSERGEPLARAMCDARGRPVLRAGDNAFTYTGG
                     RAEITVTTFGEAFGASRRRRAIDWKHLKHEYEMPRLVLASNDVTRAEWEVAVRPGEKA
                     RLGVEFSGGMASPAILAQGKTWSFPVTLQAGQRLLCRDGRNWSVLDANRATLSQGTLD
                     TPLPRLNGGPNRVVFTCENPDRARICLVKRYE"
     gene            complement(5847..7640)
                     /locus_tag="PLZ74_07860"
     CDS             complement(5847..7640)
                     /locus_tag="PLZ74_07860"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOR98275.1"
                     /translation="MKLTADQLRDKYLTFFESKGHAIISGASLIPENDPTVLFTTAGM
                     HPLVPYLLGEPHPAGRRLTNVQKCVRTGDIDAVGDSCHLTFFEMLGNWSLGDYFKKEA
                     IAWSFEFLTQHLGFSPDQLSVTVFAGENGIPRDDESIAIWKSLGIPDERIHALPKEDN
                     WWGPAGTTGPCGPDTEMFIDNGQPACGPECRPGCHCGKYIEIWNDVFMQYNKTAEGTY
                     EPLKQHNVDTGMGVERTICMMNGCETVFETELFAPLMARIKELSTVRPDEETAVRAER
                     IIADHMRTSVFILGDPKGGVKPGNIGANYVLRRLIRRSVRYAKLLGIAPGYTRTLAEI
                     VIDNYKRTYPELEENRETVIGELTAEEARFEKTLAAGQREFDKVAGALKQHGQTTLSG
                     RTAFKLYDTYGFPLEFTEELAAEQGLAVDRKGYEEAFKKHQELSKQGDATFKGGLADN
                     TVATTRLHTATHLLHKALQMVLGPHVQQKGSNITPERLRFDFSHPEKMTPGQIKQVEE
                     IVNDVIQRNLPVTMETMTIEEAKAKGATALFTAKYGEQVKVYATGDFSMEVCGGPHAA
                     ATGELGGFKIKKEESSSAGVRRIKAVITAKD"
BASE COUNT         1349 a         2462 c         2491 g         1457 t
ORIGIN      
        1 gtttctgggt ttcagggttt catccaaatc ggggtcgggg tcggtatcgg gatcgctatc
       61 gctatcggga tcgggatcgt cgacccgcga cccgcgacat tcgacccgcg acgatcgaca
      121 gggacgtccg cgtttccgac accgcaagtc gcccgtcgcc cgtcgcgggg cgacataagg
      181 tctgacggga aacgcgggcg tccctaccga atcgacgact cgacgggacg cgcgtttttt
      241 gttacggtga tcccgaattt aaaaccctgg agaatctcac atgcggaaaa tccttttggt
      301 ttgtgcgatg gctacggcgc gcctcgtttg ggggcaagtt tcagacaccg ccaaagacga
      361 tgcggcggcc gaccgggaca aggcattgcg cgcgcagctc ccgggactgt tccgggacgc
      421 cgccgagcag taccgcggca tgctgaagca actggaaggc acgcaagacg cgtttccgcg
      481 ccgctgggag aaggggagcc tcgtgaccat cgggccgaaa gagtggacca gcggcttctt
      541 cccgggttcg ctctggctcc tttatgaggc cacgcgcgaa gagacgtgga aacgcgaggc
      601 gatgcgttac accgccatgc tggagtcggt ccgccactat accggcaacc acgacatcgg
      661 ctttatgctg tactgcagtt tcggtaacgg tctgcggcta gccaaccccg acgggtaccg
      721 cgaggtcctg ctggacggtg ccgccgcgct ctgcaaacgt tatgtgccga gtctgggcat
      781 gatccgttca tgggacagtt acaaaaatcc ggtgatcatt gacaacatga tgaatctgga
      841 gttgctgatg tgggccgcga aacaaagcgg ggagccgcgt ttccgcgaga tcgccgtcag
      901 ccacgcggat cagaccaacc ggcgccactt ccggccggac ggcagcgctt accacatcgt
      961 ggattacaat cccgccaccg gtaagatctt cggctattat gccgggcagg gcgcttcggc
     1021 cgacggcccg tgggcacgcg gccagtcgtg gggcctctac ggattcacgc tgatgttccg
     1081 tgaaaccggc acccccgcct accttacgcg ggccgtcagc ctggcggact ttttgatcgg
     1141 ccaccgtaac ctgcccgcgg acgccatccc ctactgggat tacgaggcgg cgggtatccc
     1201 gaacgaaccg cgcgacgcct cggctgcagc catcatggcc agtgcgctgc tcgaactcgc
     1261 gactttcgcc gaagcgccca aggccgtccg ctaccgcgac accgctgtgc ggcaactgct
     1321 tgcgctcagt tcccccgcct atcgcgcgcc cgtcggggag aacggccact tcctcctgat
     1381 gcacggcgtc ggccacctac ccggcaacaa agagatcgac gtgccgctga attatgcgga
     1441 ttactactac ctcgaagcat tgctgcgttt ccggcgttta cagaacaact gacacgctca
     1501 catcccaaac caaccgaaag gagtccatca tgctatccga attcgtcaag agtgccgact
     1561 ggaagaacga gaaacacgtt ccggtcatcg aatgcgccga cacggtccag gccggtaagc
     1621 cgctcgccat cacgatctcc gtcggcaaag agacggccca tccgaacacg accaaccacc
     1681 atattgtctg gatcgccctg cattacgtgc ccgccggggc caaaacctca atcgagatcg
     1741 cccgctgcga gttcagtgcg cacgccgccg gagtcgaggg gcccgacaaa ggacctgccc
     1801 gcacctgcag cacggtctcg atcagcgcgg agttcgccgt ctccggcacg ctatacgcca
     1861 cggcctactg caatatccac ggcctctggg cctcggagca gccgatcacc gtcgcgtgac
     1921 cgttgccgtc taagggaaag agatgagatc ccgattaccg ctcgtgttag gaggggtcgg
     1981 cctcgcactg ttggtgcggg ccgcaccgct ggcttcggat ctgttgctct ggttgccttg
     2041 cgatgagggg ggcggcgctt ttacggagga tcgttcgcct aaccgtctgg aggcggaact
     2101 ttcgggcgtg cagtgggtca ccggaagctt cggcacggca ctgcggttca atggaaccaa
     2161 cgcgctggcc gaactgcctc ccgtacccgg actcaacggc gcggaacggt tcaccctcgc
     2221 gctctgggcc gtttgggagg atcccgcgcc gcgccgctat ccctgtctgc tgagttcgca
     2281 cacctggagc cccggcggca tgctgctttt cgtttcggac cagacctgct ccttccggct
     2341 gggacggccg ggccatcgcg ccggaacgcc gggccatgcc tggaacgaga gttccgtgaa
     2401 tctgctctcg gacctgccgc aacgccgctg gacgcacctg tgcgtcacgt tcgaccgtcc
     2461 gcacctcaca tcgtatgtcg gcggcaaacc cgtggcgcgc acgcgttggg accatgccgt
     2521 ggaagcggac gcgctgcgtc tcggcggctg gtccggcgcg gtttgccata acgggctgat
     2581 cgacgatctg cggatctatg gccgggccct gacaccctcc gagatcacgg cgctggccaa
     2641 cccctccgac cgcgccgacg cggcttggaa accggctacg gaacgggaac gtgcgcccga
     2701 accgccgttc gcgcgtttcc ggacacggca cgcggaactg accctgaacg cccggggccg
     2761 ggtcacctcg ttgcgcaatc gcgcaagcgg ccgcgagctg ctggttcgtc ccacaccgct
     2821 ggtttcggcc cgcttgcgga acggacagcg aatcacggcg cgttccgtaa cgcgtcaggg
     2881 tgacgccttg actttcgggt tcgcgaccca cgccggttcg gcggtcgtcg cgattgatgc
     2941 gcaccgcgag ttctttaact tcaccctgcg tacgcattcg ctgaccgacg cgacggaact
     3001 cgtcttttgc gaactgccgg tccgtcccac ccgttaccgg ggcggcatgg ccaacctgct
     3061 ctcggatgat gaagacgggg tctgtctgcg cggctatgac ctgccggtgg agatgcggat
     3121 cgccggggac gtcctgcgtg tctccgccgc cgtaccgccg ggtccctccg gttggcgcgc
     3181 cgggctggcg gccgggccgc gcgataccct gacgaccagc ctgcgcgcca tggcccgcca
     3241 cgccggagtg ccgatgtcgg aaaacagcgg cccgtggtcg ctggaggcag aggccacacg
     3301 cggatcctac ctgttcgcca atctcgcgca tgccgccacc gatgactgga tcgaggtcgc
     3361 gcgacgcggc ggtttttcca ccctgcacct ccacggctgg tggcaaacgc tgggccacta
     3421 tgcggtgcgc acccaatatt atccgcgcgg gctggacgat atgcgggaca cggtggagcg
     3481 gatccatgcc gcaggattgc gcgcgggaat ccacacgctc accgcctgca tcgacacgcg
     3541 tgacgcgtgg atcacgcccg aggcgagtcc gcacctgatc ccgttcgaca cgtacacgct
     3601 ggcccgtagc gtctcgccga cggatacggt gctctatgtc aacgaacccc catcggcgcg
     3661 tcacgatgtg gtcttcactt actccggcaa cggaaacgcg atccggatcg gcagcgagat
     3721 catccaatac gccgccatca cacgcgaacc gccctacgct ttccgtgact gtacccgggg
     3781 cgcgttcaag acccgccccg cagcccatgc cgcaggcgca caggctgatt atctgcagca
     3841 gcgttacaac gctttctatc cgcaacctga ttcgccgctg gcggacgagt tggcggatcg
     3901 tatcgcgcac gtcttcaaca cctgcaaact ggatcagctc tatttcgacg gctccgaggg
     3961 gatgatgagc cgatacggta tcgatgccat gcgccacaag atcatgcgtc ggctggaggg
     4021 gaatccgctg atcgaggcga gttgccacgg tgcgcataac tggtggttcc attcgcgtct
     4081 cggggcttgg gaccatccgg tctgggcacc gaagcggttc catgacgatc acgttgcggt
     4141 gtgcgcgcgt taccgcgctt cggatctgct cgccccgcag atgggctggt gggcaccgcg
     4201 catggcgacc cccttcgcgc gcgggcacca tctcgacgag atggagtatt tcgctggcaa
     4261 gaatctggga ctcgacgccg ccatgtcgat ccagggggta aatgtcaatc aagcgatcct
     4321 cccgtatcat ctggaacgac agttcacgct gctgggctgg tacgagcatc tgcgtctggc
     4381 gcgctatttt gacacgcaga ccgtggcgcg cctgattgtg ccgggtgcgg aattccggct
     4441 gcggcagacg cgcgatggca tatggcagtt cacgccggtc acgatgcgca cacaccgggt
     4501 caccggaccg gatgacggtt ccgccgcctg gcgggtcacc catccccatg cgcaacaacc
     4561 gctctgtctg cgcgtggagg ctctgtacga cgccgcgccg gcagcaaccc ctccggcggg
     4621 caagtcgctg gtcgggacga atgattttgc ggcgttcgcg gtatcgaccg cctctgcggc
     4681 cgtgcgcctg aatgtgtcag aagtatctga ggaaacgcgc ggcggcaacc gccatctgcg
     4741 gctgcgggcg gagaaccgcg gcacgacatc caccggcgcg tgggctcgcg cggtgctcac
     4801 cttccccgcg ccgtaccgca atttgagcgg caccggtgcc ttcggcatgt gggtgaaggg
     4861 tgacggttcg ggtgcgttgc tcaacctcca ggtcggttgc ccgcgcgagt tcatgggcgg
     4921 cctgtccgac cattatgtca cgctggactt cacgggctgg cggtatgtca cgctgttgac
     4981 gcgcgagcgc gacaccgcgc gtatggacga acacgtctgg ccgtaccgga aggcggggtc
     5041 gcatgcgctc ttccgcacga acctcgacat ggcccacctc tcacaggtca gcctgtatct
     5101 caatgccctg ccagcgggcg cacatgttga ggtcaccgta agtccggtgg aagccctgcc
     5161 ggtgcggtcg gtcgacatga agcggccggt ggtgaccgtg aacggacaag gtctgacggt
     5221 gccgttcacg ctggcctccg gcgaattcgc cgagttggaa ccggacggcc tcttcacgca
     5281 ctacagcgag cggggcgagc cactggcgcg cgcgatgtgc gacgcaaggg gacgaccggt
     5341 tttacgggcc ggcgacaatg cgttcaccta cacgggcgga cgagcggaaa tcacggtcac
     5401 cacgttcggc gaggcattcg gcgcatcccg caggcgacgc gcgatcgact ggaaacatct
     5461 gaagcatgaa tatgagatgc cgcgcctggt gctcgcctca aatgacgtga cccgcgccga
     5521 gtgggaagtg gctgtgcggc ccggtgaaaa agcgagactg ggcgtcgagt tctctggcgg
     5581 aatggcgtcc cccgcgatcc ttgcgcaggg caagacctgg agttttccgg tcacgctcca
     5641 agcggggcaa cgcctgttgt gccgcgacgg acgaaactgg tcggtgctgg acgcgaaccg
     5701 cgcgacgctg tcgcaaggta cgctcgatac gccgttgccc cggctgaacg gtggtccgaa
     5761 ccgcgtggtt ttcacatgcg aaaatcccga ccgcgcgcgg atctgtctgg tcaaacggta
     5821 cgagtgagcg attggattcg tccgcgttaa tctttggcgg tgatcacggc cttgatgcgg
     5881 cgcacgcctg ccgaggaaga ctcttccttc ttgatcttga agccgcccag ctcgccggtg
     5941 gcggcggcgt gcggaccgcc gcagacctcc atcgagaaat cgccggtggc gtagaccttg
     6001 acctgctcgc cgtatttggc ggtgaacagg gcggtcgcgc ccttggcctt ggcctcttca
     6061 atggtcatgg tctccattgt gaccggcaga ttgcgctgga tcacgtcgtt gacgatctcc
     6121 tccacctgct tgatctgccc gggcgtcatc ttttcggggt gtgagaagtc gaagcgcagg
     6181 cgctcgggcg tgatgttgga gcccttctgc tggacgtgcg ggccgagcac catctgcaac
     6241 gccttgtgca acaggtgggt ggcggtgtgc aggcgcgtgg tggcgaccgt gttgtcggcc
     6301 agccctccct tgaaggtggc gtcgccctgc ttggagagtt cctgatgctt tttgaacgcc
     6361 tcctcgtaac ctttgcggtc cacggcaagc ccttgctcgg cggcgagttc ctcggtgaac
     6421 tccagcggga agccgtacgt gtcgtagagc ttgaaggccg tgcgccccga aagcgtggtc
     6481 tggccgtgct gcttgagtgc gcccgcgacc ttgtcgaact cacgctggcc ggcggcgagc
     6541 gttttttcaa aacgcgcctc ctcggcggtc agttcgccga tcacggtttc gcggttctcc
     6601 tccagttcgg gataggtgcg cttgtagttg tcgatcacga tctccgccag cgtgcgggtg
     6661 tagccgggcg cgatgccgag cagcttggca tagcgcacgg agcgtcggat caggcggcgc
     6721 aacacatagt tcgcaccgat gttgccgggc ttgacgccgc ccttggggtc gccgaggata
     6781 aagaccgagg tgcgcatgtg gtccgcgatg atgcgctcgg cgcgcacggc ggtttcctcg
     6841 tccggccgga cggtcgacag ctccttgatc cgggccatga gcggcgcgaa gagttcggtc
     6901 tcgaagaccg tttcgcagcc gttcatcatg cagatggtac gctcgacgcc catgccggtg
     6961 tcgacgttgt gctgcttgag cggctcataa gtaccttccg ccgtcttgtt gtactgcatg
     7021 aagacgtcgt tccagatctc gatgtacttg ccgcaatggc aaccgggacg gcactccggg
     7081 ccgcaggcgg gttgaccgtt gtcgatgaac atctcggtgt caggcccgca ggggccggtg
     7141 gtgccggccg gaccccacca gttgtcctcc ttgggcagcg cgtggatgcg ttcgtcgggg
     7201 atgccgaggc tcttccagat agcgatcgac tcatcatcgc gcggaatgcc gttttcgccc
     7261 gcgaagacgg tgacactgag ctgatccggc gagaagccga ggtgctgcgt gagaaactcg
     7321 aacgaccacg cgatcgcctc tttcttgaaa tagtcgccga gcgaccagtt gccgagcatc
     7381 tcaaagaagg tcaggtggca ggagtcgccc acagcgtcaa tgtcgccggt gcgcacgcac
     7441 ttctgcacgt tggtcaggcg tcggcccgca gggtgcggct cacccagcag atagggcacg
     7501 agcgggtgca tgcccgcagt ggtgaagagc acggtcgggt cgttctccgg aatcagcgag
     7561 gcacccgaaa taatggcatg gcctttggat tcgaaaaagg tgagatactt gtcgcggagt
     7621 tggtcagctg tgagtttcat gattgttcct ttaaaacgga taaagagaaa tggaagcttc
     7681 aatatgaagc cgcacttgat aacattggaa gaatcgcaag gcatcttcct tgtgagatct
     7741 gttgaagacc ggatgccgt
//
LOCUS       DAOSRB010000134         7627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9021_length_7627_cov_7.025225, whole genome shotgun sequence.
ACCESSION   DAOSRB010000134 DAOSRB010000000
VERSION     DAOSRB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9021_length_7627_cov_7.025225"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1546)
                     /locus_tag="PLZ74_07865"
     CDS             complement(179..1546)
                     /locus_tag="PLZ74_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98276.1"
                     /translation="MVRYESMRTSEQAATRSDYTPWRATTFYIPLLLQAVSQSLTYPL
                     VAAIVSHGEHGVVELAAFAQGQAVMFVIGALGSGLLTTGMVFGRTMEGFRTFTRINFA
                     FCCVLLTLQALTCVHPFDTFVFREILGLDPAMAGTARAVLFYSIPLHCLFLLRIPGLV
                     ALYNARASIVANWGTLCRIVLTACLSPLFVRLGWVGHEMGVLAMTGPVMAEYGLINWM
                     AKPYIRRLEPSAGQPEDSRTQFLFTVPLSFGGVLLAMAGFMIGAFISRAAEPARMLPI
                     HYVTIGIVNPVGFAALRMQAVVLAFPPRDRHDNSVFRFALAAGCMLALFPLIGQIPAV
                     AQWYFGKVQNVPACDIPLAMRAMLYMSVLPVIQSLRGHAEGLAAWRKRPNAILAGQAV
                     NLATLVCTLFVTLSFGVPGYLMGVTAILASTLMTWVTIRMGLVWAEMEDAFDEPSRPV
                     RSMEG"
     gene            1666..2301
                     /locus_tag="PLZ74_07870"
     CDS             1666..2301
                     /locus_tag="PLZ74_07870"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOR98277.1"
                     /translation="MKILITAGPTREHLDPVRFLSNRSSGRMGFALARAAAERGHEVT
                     LIAGPVALPTPTGVTRTDVVSAREMLAAVERLLPAHDALVMSAAVADWRPKRNSPAKL
                     KKAAMTPVLELEPNPDILAAVRDLKGKRLFVGFAAETGDPLGEARRKLEAKGLDLIVA
                     NDVTLPDAGFEVETNRVTLIAKNAAPVPLPLLSKYEVGLRIVTWLESACSL"
     gene            2446..3390
                     /locus_tag="PLZ74_07875"
     CDS             2446..3390
                     /locus_tag="PLZ74_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOR98278.1"
                     /translation="MDALSLYDLMLTIRFFEEAIERLFLEGRIMGTAHTCIGQEAVAV
                     GVAAALEPCDALTTTHRGHGHFLARGADPGRALAELFGRAVGYSRGLGGSQMMMDVSR
                     GFYGANGITGGSIPFATGLALEAQYRKTGRVTACLFGDGASNQGVFHESLNLAALWKL
                     PVLYIVENNGYAMSTATARGLADPCVAGRAAAYGIPGERVDGNDVLAVRDIVARHAAR
                     ARAGKGPSLIECVTYRLSGHSRGDPRVYRSREEEAQAWSRDPIRLFEARLLAEGVLGE
                     ADVAARHEAAQQRIETAIAFCEAAELPDPEILGEPVFA"
     gene            3414..4388
                     /locus_tag="PLZ74_07880"
     CDS             3414..4388
                     /locus_tag="PLZ74_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOR98279.1"
                     /translation="MRTVTFTQILRETLAAEMRRDASVILIGEDIGVYGGSFGVTRGL
                     LDEFGPERVRDTPISEQAITGVAIGAAAAGLRPVLEIMFMDFITLAVDQLVNMAAKLY
                     PIYGVRCPLVVRTPPGAGRGYGATHSQSLERLFTGVPGIKIVAPSDATDAAGLLQTAI
                     RDDNPVLFLEHKLLYPQRFEMEDGLPPPIPFGTSRFVREGTDVTLVAWSYMTQVARQA
                     AAALAEEGIEAEVVDLRTLNPLDLGIAVESAQNTGRVILVEEGPQTGGFAAEIAARLA
                     ERAHGALTCPVRRLAAPDWPVPSAKNLEAVWMPSADAIAALARSMVDA"
     gene            4385..5788
                     /gene="dcm"
                     /locus_tag="PLZ74_07885"
     CDS             4385..5788
                     /gene="dcm"
                     /locus_tag="PLZ74_07885"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOR98280.1"
                     /translation="MMKEKHAYNENVPMPVKKQKKGSYIEPGTVFPHRKRYTCIDLFA
                     GCGGLSLGLEQAGFTPILFSEINLNAAETYMANRKGKEIIPVGDVYSLTDANLAVLKS
                     YWRLHRGIDDVDLVCGGPPCQGFSGIGHRRTFRLEKKDIPSNHLFQEMIRVISAVRPK
                     IFLFENVKGLLVSKWSAEGRNGEIFKQVHSEFSRIPGYSVRYELVHAKDYGVPQNRPR
                     VLMVGLRQDVFWKSEQMSFSLSREVDLAEYDSGRLELPKPSAIAHGFLPKSSGIPPTM
                     CELLSDLEDPNYFSKTETDSYPSEPQNEFQKMLRTLPNGKTLGRGDALTEQEYSKHRP
                     EIRKKFEYMIANNGEIPAAMRTKKFAQRVLPKTWPDNGPNITACSLPEDYVHYCQPRG
                     PTVREWARIQTFPDWYIFTGPRTTGGRRRAGDPSLGIWTRDVPRYTQIGNAVPVLLAQ
                     KVGEHLVKLLNREVIPS"
     gene            5785..6963
                     /locus_tag="PLZ74_07890"
     CDS             5785..6963
                     /locus_tag="PLZ74_07890"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOR98281.1"
                     /translation="MMNMRPLTAKESQNLAVLNAAGIESVPLFLTATGYRKAICDATD
                     PMRAFLLKHSIHDYSTQRQGPDNKVIRQVHFLAENGLRFMDMAFYRPVTKKGDPRFWV
                     YGWKAFVDADNVLAFFFDNGSLCIANLSTVNLAAPAINTWLTRRIQQSERISKELLSR
                     LCELARRPLPATCSGDTAIGRAVEAALGIPINSSGNPDYKGIELKAKRERTSAKRNGL
                     FTCVPDWTLSHIKDFKTFLDRFGYQRGDDFKLYCTVSTKAPNPQGLLLSLGDADRFLW
                     ENVRKNGSDERLMVWPLSKLEDRLTTKHKETFWIKADSVRDNSGREFFCLKSVTHTRN
                     PNVPQFSRLLSDGSITLDHLIKRTPSGGVKEKGPQFKLAPDKFSELFLGEPKRYRLAE
                     "
     gene            7092..>7627
                     /locus_tag="PLZ74_07895"
     CDS             7092..>7627
                     /locus_tag="PLZ74_07895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR98282.1"
                     /translation="MLYSLITLSYNKLACTRRCLEALVRHTRSVVPWELIVVDNGSTD
                     GSREWCETGLIELGAACGVAVTVLRNHGRNNCSAARNLGVAAARGSHLVFVDNDVAPR
                     TRHWLDILRGVLESEPQAGMAGAKLVYPWPPYAIQCAGVGISQRGHVCFLGRGESRED
                     ARYTVRREVQCLISACLM"
BASE COUNT         1593 a         2191 c         2325 g         1518 t
ORIGIN      
        1 gcgacattcg acccgcgacg atcgacaggg accaagagtt caaaaagagc gtcgggcgca
       61 ccgcaaaacc caatgtttgc ggggctttgc tggaattcta cccgttcaac tgctcctttt
      121 agggtaatct gtggatcagg ttgtcccagc gtttgtgcct gctctgcggg cggcgggctt
      181 atccctccat actgcgcacc gggcgcgagg gttcgtcaaa ggcatcctcc atctccgccc
      241 aaaccaaccc catccggatc gtcacccatg tcatgagcgt ggacgcaagg atcgccgtca
      301 cccccatcag gtatcccggc acgccgaagc tcagggtcac aaaaagcgtg cagaccagcg
      361 tggccagatt gaccgcctgt ccggccagaa tcgcattcgg ccgcttacgc caagccgcca
      421 acccctcggc atgtccgcgc aacgactgga tcaccggcag aaccgacatg tacagcatgg
      481 cgcgcatggc gagcggaatg tcacacgccg gcacattctg gaccttcccg aaataccact
      541 gcgccacagc cgggatttgt ccgatgagag ggaataacgc caacatgcag cctgcggcca
      601 gcgcaaaccg gaagaccgag ttgtcatgcc ggtcacgcgg cggaaaggcc agcaccaccg
      661 cctgcatgcg caatgcggcg aaccccaccg gattgacgat cccgatggtg acatagtgaa
      721 tcggcagcat gcgcgcgggt tccgccgcgc gcgagataaa cgcgccgatc atgaagcccg
      781 ccatggccag cagcaccccg ccgaacgaga gcgggaccgt aaacaggaac tgcgtccggg
      841 aatcctccgg ctgcccggcg gacggttcca atctgcggat atacggtttc gccatccagt
      901 tgatcagccc gtattccgcc atcaccggcc cggtcatggc cagaaccccc atctcatgtc
      961 ccacccagcc tagtcgcacg aacagtggcg acaggcatgc cgtcagtacg atccggcaca
     1021 gggttcccca gttcgccacg atactcgcgc gcgcgttgta aagcgccacc aaccccggaa
     1081 tgcgcagcag gaaaaggcag tgcagcggga tactgtagaa caggaccgcg cgggccgtgc
     1141 cggccatcgc cggatccagc cccagaatct cccggaaaac aaaggtgtcg aacggatgca
     1201 cgcatgtcag cgcttgcagc gtcagcagca cgcaacagaa cgcgaagttg atgcgcgtga
     1261 aggtgcggaa cccttccatc gtccttccga acaccatccc ggtggtcagc aggccgctgc
     1321 ccaacgcgcc gatcacaaac atcaccgcct gcccctgcgc gaatgcggcc agttccacga
     1381 ccccatgttc gccgtgcgac acgatcgccg cgaccagcgg ataggtcagg ctctgcgaga
     1441 ccgcctgaag taaaagcggg atgtagaatg tggtggcccg ccacggtgtg tagtcggatc
     1501 gggtcgcagc ctgctcggat gtccgcatgg attcatatct taccatgccc gccccggaaa
     1561 tccaatgctc atcgaaccaa ggggatgtgc ctctcggaac gattcgccaa aatgggtatg
     1621 ccccggttct gtttctttct gttatactgg cgagcatccg aaacgatgaa aatcctgatc
     1681 acagcaggcc ccacacgcga gcacctcgac cccgtgcgtt ttctcagcaa ccggtcgagc
     1741 ggcaggatgg gcttcgccct tgcgcgggct gcagcggaac gcgggcatga ggtcaccctg
     1801 atcgccggac cggtcgcgtt gcccaccccg accggagtga cccgcacgga tgtggtcagc
     1861 gcgcgcgaga tgctcgctgc tgtggagcgg cttctgcccg cgcacgacgc gctggtgatg
     1921 tctgcggccg tggcggattg gcgtcccaaa cggaacagcc ccgcgaaact caagaaagcc
     1981 gccatgacgc cggtgctcga actggaaccg aatccggata tcctcgcggc agtgcgcgac
     2041 ctcaaaggga aacgactctt tgtcggattc gcggctgaaa ccggcgaccc tttgggcgaa
     2101 gcccggcgca aactggaagc caaagggctg gatctgattg tggccaatga cgtgaccttg
     2161 ccggatgcag ggttcgaggt tgaaaccaac cgcgtcacac tgatcgcgaa aaacgccgcg
     2221 ccggttccct tgccgcttct gagcaaatat gaggtcggcc tgcggatcgt tacgtggctg
     2281 gaatcggcct gctccctgta acccggttgg gacgcctccg atttggatga tcggtctgag
     2341 ccggcaaccc gaaacaaacc ggggtctcgc gacggtttgc cctgatggag caagtgtgct
     2401 atacttggca gaccattttg agggagaagc aacagcaggg ggggcatgga cgcgctttcg
     2461 ctatatgatc tgatgttgac gatccgcttc ttcgaggaag cgattgagcg cctgtttctg
     2521 gaagggcgca tcatgggtac ggcgcacacc tgcatcgggc aggaggcggt ggcggtgggg
     2581 gtggctgccg cgctggagcc gtgcgacgcc ctgaccacga cccaccgcgg gcacggacat
     2641 tttctggcgc gcggtgcgga tcccggtcgg gccttggccg aactcttcgg ccgcgccgtc
     2701 ggatattcgc gcggcctcgg cggcagccag atgatgatgg acgtgtcgcg ggggttctac
     2761 ggtgcgaacg gcattaccgg cggcagcatc ccattcgcca ccgggctcgc gctcgaagcg
     2821 caataccgca agacgggacg ggtgacggcc tgccttttcg gggacggcgc ctccaatcag
     2881 ggcgtgtttc acgagtcgct gaacctcgcg gcgctgtgga agttgccggt gctgtatatt
     2941 gtggagaaca acggctacgc gatgtcaacc gccacggcgc gcgggcttgc ggatccctgt
     3001 gtggcgggcc gcgcggcggc ctacggtata cccggcgaac gggtggacgg gaacgacgtg
     3061 ctggcggtgc gcgacatcgt ggcgcgtcat gcggcgcggg cgcgcgccgg caaggggccg
     3121 tccctgatcg agtgcgttac ctaccggctt tcgggccatt cgcgcgggga tccgcgcgtg
     3181 taccgcagcc gggaggaaga ggcccaggcc tggagtcgtg atccgatccg tcttttcgaa
     3241 gcgcgtctgc ttgcggaggg ggtgctcggg gaggcggacg ttgccgcgcg gcacgaggcc
     3301 gcgcagcagc gcatcgaaac ggcaatcgcc ttttgcgagg cggcggaact accggatccg
     3361 gagattctgg gcgaaccggt ttttgcctga cagagaggag gtgtgtgctg cctatgcgaa
     3421 ccgtcacgtt cacccagatt ttgcgcgaga cgttggccgc cgagatgcgg cgtgacgcct
     3481 cggtgatcct gatcggcgag gatatcggcg tatacggcgg ttccttcggc gtcacgcgcg
     3541 gtctactcga tgagttcggg cccgagcgcg tgcgagacac gccgatctcc gaacaggcga
     3601 tcaccggggt cgcgatcggt gccgcggccg cgggcctgcg tccggtgctt gagatcatgt
     3661 tcatggattt catcacgctg gcggtggacc agctcgtgaa catggccgcc aaactctatc
     3721 cgatctacgg ggttcgctgt ccgctggtgg ttcgcacgcc gcccggcgcg gggcgggggt
     3781 atggcgcgac gcattcgcaa tctctcgaac ggctgttcac cggtgtgccc ggcatcaaaa
     3841 tcgtggcgcc ctctgacgcg acggacgcgg cgggactgtt gcagaccgcg atccgggacg
     3901 acaacccggt gttgttcctc gagcacaagt tgctgtatcc gcagcggttt gaaatggagg
     3961 acgggctgcc gccgccgatc cccttcggca cgtcgcggtt cgtgcgcgag ggcacggacg
     4021 tcacgctggt ggcgtggtcg tacatgacgc aggtggcgcg gcaggctgcg gcggcgttgg
     4081 ctgaagaggg gatcgaggcg gaggtggtgg atctgcgcac ccttaatccg cttgacctcg
     4141 gcatcgcggt cgaatcggcg cagaacacgg gtcgcgtgat ccttgtggaa gaaggccccc
     4201 aaaccggcgg gttcgcggcc gagattgcgg cgcgactggc tgagcgggcg cacggcgcgt
     4261 tgacgtgccc cgtgcggcgt ctggccgcgc cggactggcc ggtgccctca gccaagaatc
     4321 tcgaagcggt ctggatgccg tccgcagacg cgattgccgc tctggctcgt agcatggtcg
     4381 acgcatgatg aaggaaaaac acgcttacaa tgaaaacgtg cctatgcccg tgaaaaaaca
     4441 aaaaaaagga tcttatatcg aaccgggtac ggtttttccg catcgcaaac gctacacctg
     4501 tattgacttg tttgccggat gcggcgggtt gtcgcttggt cttgaacagg cgggtttcac
     4561 tccgatcttg tttagcgaaa tcaacctcaa tgccgccgaa acctacatgg caaaccgcaa
     4621 gggcaaggaa atcattcctg ttggggacgt gtatagtctg accgatgcca atcttgcggt
     4681 tctaaaatcc tattggaggt tacatcgtgg aatcgatgat gttgatttgg tttgtggcgg
     4741 cccgccttgt caaggattca gcggaatcgg gcatcggcgc acattccgtc tcgaaaaaaa
     4801 agacattccg tcgaatcatt tgtttcagga gatgattcgt gtcatcagcg ctgtccggcc
     4861 caaaatcttt ctttttgaga atgtcaaagg attacttgtt tcaaaatggt ctgcagaggg
     4921 aagaaacgga gaaattttca agcaagtgca cagtgagttc tcacggatcc cagggtattc
     4981 cgtccgctat gaacttgttc atgccaagga ttatggtgtt ccgcaaaatc gcccccgtgt
     5041 gctgatggtc ggtttgcggc aggatgtctt ttggaaatcg gaacagatgt cgttctctct
     5101 ttcaagagaa gtagatctag ccgaatatga ttcagggcgg cttgagttac ccaaaccaag
     5161 cgcgatcgcg cacggatttt tgcctaaatc gtcaggaatc cccccgacaa tgtgcgaatt
     5221 gctttcggac ttggaagacc ccaattattt ctcaaaaacg gaaacggact cgtatccgtc
     5281 tgagcctcaa aacgaatttc aaaagatgtt gcgaacgctt cccaatggaa aaacgttggg
     5341 gaggggagat gcgttgaccg aacaggaata ttcaaaacat agacctgaaa tacgaaagaa
     5401 atttgaatat atgattgcga acaacggaga aatccccgca gcgatgcgga ccaagaaatt
     5461 cgctcaacgc gtgttgccga aaacatggcc tgacaatggg ccgaacatca cggcttgttc
     5521 tttgccagaa gattatgtgc attattgcca accgagaggg ccaaccgttc gtgaatgggc
     5581 aagaatccag actttcccgg attggtatat cttcacgggg ccacggacga ccgggggacg
     5641 tcgacgtgct ggggatccgt cgcttggcat ctggacgcgc gatgttccac gctacacgca
     5701 aatcggtaac gcagttcccg tgttgctcgc acagaaggtc ggagaacatc tggtaaagct
     5761 gcttaatcgg gaggttatcc cgtcatgatg aatatgcgtc cacttactgc gaaagagtcg
     5821 cagaaccttg ccgttctcaa tgcagccgga atagaaagtg ttccgctttt cctgaccgcg
     5881 acgggatatc gtaaggcgat ttgcgatgcg acggacccca tgcgggcgtt tctgctcaag
     5941 cattcgatcc atgattattc cacacagaga caagggccgg acaacaaagt catccgacaa
     6001 gttcacttcc ttgccgagaa cggcctacgt ttcatggata tggcattcta tcgtcctgtg
     6061 accaagaaag gcgatccgag attttgggtg tacggatgga aagcgtttgt ggatgcggac
     6121 aatgtgcttg cgttcttttt cgataacggt tcgctctgta ttgccaatct gtcgaccgtg
     6181 aatctggctg cccctgcgat caacacatgg ttaacccgcc gcattcagca aagcgaacgc
     6241 atctcgaagg aactcctgtc gagactgtgc gagcttgccc gtagaccgct ccctgcgact
     6301 tgctcgggtg acaccgccat cgggcgtgcc gttgaagccg cgttgggcat tcccatcaac
     6361 tccagcggga atcctgatta taaaggtatt gaactcaagg ccaaacgcga gcggacatca
     6421 gcaaagcgca atggcctttt cacttgcgtt ccagactgga ctttaagcca cattaaggac
     6481 ttcaaaactt ttttggatcg cttcggatac cagcgcggcg atgacttcaa actttattgc
     6541 accgtctcca cgaaagcgcc aaatccgcaa gggttgttac tctcgcttgg ggacgcggac
     6601 cgcttcctgt gggaaaacgt ccgaaagaac ggtagtgacg aacgcttgat ggtctggcct
     6661 ctttccaaac ttgaagaccg cctgactacc aaacataaag agaccttttg gatcaaggct
     6721 gattccgtcc gcgacaactc gggacgtgaa ttcttttgcc tcaaatccgt aacgcatacg
     6781 cgcaacccca atgttccgca gttctcgcgg cttttatccg acggtagtat cacgctagac
     6841 cacctaatca aacgtacccc ttcgggtggg gtaaaagaaa aaggcccgca gttcaaactt
     6901 gcgccggata agttttccga actcttcctt ggcgaaccca aacgctacag actagcggaa
     6961 taacgatgaa cgccttgcga gaaagatttt gtcgatgatt tttgctttat ttttcaaccc
     7021 aacaagaagc aacttgacgt ttaggccgca acaaaggacg gtaacccgtt atctggcata
     7081 tgtgtaatcc tatgctctac tccctcatca cgctttccta caataagttg gcctgtacgc
     7141 ggcgctgtct ggaggcgctg gtgcgccata cgcgttccgt cgtgccgtgg gaactgatcg
     7201 tggtggataa cggctcgacc gacggcagcc gggagtggtg tgaaaccggg ctgatcgaat
     7261 tgggggcggc ttgcggggtg gcggttaccg tgttgcggaa ccacgggcgc aacaactgtt
     7321 cggccgcgcg caatctaggg gtggcggcgg cacgcggcag ccatctggtt tttgtggaca
     7381 acgatgtcgc gccgcgcacg cggcattggc tggatatcct gcggggggtg ctggagagcg
     7441 aaccgcaggc gggcatggcc ggtgcgaaac tcgtctatcc ctggccgccg tatgcgatcc
     7501 aatgcgcggg ggtcggcatt tcgcaacggg ggcatgtctg ttttctcggg cggggcgagt
     7561 cgcgggagga cgcgcgttac acggtgcggc gtgaagtgca gtgcctgatc agcgcctgcc
     7621 tgatgat
//
LOCUS       DAOSRB010000135         7613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9042_length_7613_cov_7.691320, whole genome shotgun sequence.
ACCESSION   DAOSRB010000135 DAOSRB010000000
VERSION     DAOSRB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9042_length_7613_cov_7.691320"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1679)
                     /locus_tag="PLZ74_07900"
     CDS             complement(87..1679)
                     /locus_tag="PLZ74_07900"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOR98283.1"
                     /translation="MTKKITVAGIAGFVLASMFTIGCVLKCGVRDVPVRTLELPSGPG
                     NPRNSEGAFVTLKDGRILYVYTHFTGGGGDHDSAHLAGRYSSDGGRTWTPQDRVIVPN
                     EGGMNVMSVSLLRLQSGEIALFYLPKNNEQDCRPVMRLSRDEGETWGAPVMCITDEVG
                     YYVLNNDRVIQLKGGRLVVPVCLHWPQGASKADWQGVLMCYLSDDNGKTWRRSKTVQK
                     GYDTAGKRIITQEPGVVELKDGRVMMFIRASGGCQYLAWSSDGGDTWSAPVASEIKSP
                     VSPASIKRLPSTGDLLLVWNDHANIPAGLHGRRVPLSTAISKDDGKTWQNTKVLEGNP
                     KGHYCYIAIHPVQDAVLLGYCAMSCLAHSRVTRVPVPWFYAPSPVEKATPVPPLFGQA
                     AKGPFERLETRLGVWTAEAGHAEVYSYARGTGVRIQGGEDRQVTLTLAQPVACQALKL
                     GVERFTIRAPYALVMEAKVDDGWQKVWEQGEKTPVGNAQAVTGTAGKSVTASQFRFRC
                     TSQLGAIIVDMPDAVFNGFFND"
     gene            complement(1676..7414)
                     /locus_tag="PLZ74_07905"
     CDS             complement(1676..7414)
                     /locus_tag="PLZ74_07905"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HOR98284.1"
                     /translation="MNTRHTILFAGKPVRRVLSAVAAFSVLMTSMPVHALDWANATPV
                     AGGITGGTVGNVTTINQAEQAGIINWTDFDIAAGEIANFSQPDAAAVTLNRITSSMDG
                     TQIAGQINANGGVWVVDPNGVFIQNGARINVGAAFVAATMDISNEDFLAGNMNFDGTV
                     SGTGPVQVDSGASIAAGTLAALLGGEVSMNGVLTTPQAVLAAAGDSIIIDTVNGGTIS
                     ITLAGDAANDMQLGGTFSGDVTAAVEGQGIAQDLVGALEIAGTAELTASGAVTLDNAA
                     NDFQDGVTAHAGLSLKLADTDDIILTDVDTVNGAITVTAGGQITATDVAGLTDNDAND
                     IVLTGEGIAVGVISAGAGTAADVVLDAGTGAITDLQADTVTVGAQGFAQNAGRTVNVV
                     ADQLGMTAQGDIGAAGNPLDLTVKTVAASSANGSVYLYETDMLTVGTVGTVSGVTAGQ
                     NAKVETINGTLTVGQTVTATAGDLLLAANGAGSDVILNAAAGAAADNVTVLASDAFRQ
                     NADITAGGTIDVRTTGNIVMAPDTTSASGGSAGDNIRYFSSNGAVTMGHLNAGDGLVA
                     VDASRGIVSAGGAVNVTASGAQFSSQGGDIGAIPGIGNGPVLTDVDVLAAHSYGSQNF
                     IQVAAGGDVTVGTVGTPDDPETAGVDERAFVNRVGLDSSLTEVYLDAKSDLQSESGDI
                     RLATEWGAITVSDGLADAQSSDMDGVGVRAAGNILLDAWSMNGNVDGDVTLNAAVVSD
                     AGSISVLAARNIAQNVSGSAKGDLLVVSGDGTIDVEAADGAIVMADGTLGQTDGGHIR
                     YAASGGITLGLLDARTREARDVAEDGSDNDADSLVDSADPDEQNWNRDTDWGLVSVWD
                     DTGSVTDGNGGARNIIARGAVFTVPAGGIGEAEGVGGGPVDLQVGTLAALSQGNQYFI
                     QAPDGRDLTIGTVGDGGEQINYVMFSPVGVGSWDIERMSDLESTAGSVKVGVRNGSLT
                     VNDGEPMGPRPEMDGVGVRAAEDVLLDAWSMNGNVDGDITLNAAVVSDAGSISVLAAR
                     NIAQNVSGSAKGDLLVVSGDGTIDVEAADGAIVMADGTLGQTDGGHIRYAASGGITLG
                     LLDARTREARDVAEDGSDNDADSLVDSADPDEQNWNRDTDWGLVSVWDDTGSVTDGNG
                     GARNIIARGAVFTVPAGGIGEAEGVGGGPVDLQVGTLAALSQGNQYFIQAPDGRDLTI
                     GTVGDGGEQINYVMFSPVGVGSWDIERMSDLESTAGSVKVGVRNGSLTVNDGEPMGPR
                     PEMDGVGVRAAEDVLLMTGGGADSDVILNAAVAAGDDVTLLASGNIEQAAAGDVTAAA
                     GDVLVEAGAGIVMADGATAAAGDDLRYEAKGGDAKITGLKSTAADGAVMVKAAGGITD
                     AGDMARDVEASAAQLVAGGSVGQKSGEGNGPLDLAVGTVAAKGGDAGSVYLSEADALL
                     VGTVAAVDVNRVKMDSTSDATADGAALSGAVAGQNVKIENGVGAAGGLTVDQAVTAAS
                     GDVLLSAKGGGLSLNAAVTAGDDVTLLASRGIIQSGGITAGGTIDVETAGGSISLAAV
                     SQTSGGNIRYAASGDVVLDGLLDAGAGDVAVEAGGDITDNMGMIIVNANGFAIPNDVV
                     NIVADTVSFFAGGNIGDTGAGTSGTRPFNPITVRANGVAAEGKNIAICGKENLTIASV
                     PAVSVTRLTLDDVDQTATSPALSGINAGTGDVNLMADNSVIDGTGAGTDITAGTLTVS
                     AGHIIGAGGGQNTIDPLEVRLNGGHGPYDIWVNNVAGSPDKVVYAFLNTVGGKSSPVA
                     AATQDSGALIFVNGQYVGGDPESVQLFAAVEAFPAETPELKSRQGVFGDPFFLHDQMD
                     INEPVALGLIDLILTGKATITGAPEIPADAYREIFSGGLSPSTSFWFGTGTDDKQEEA
                     ETEEKGLEKGEV"
BASE COUNT         1345 a         2840 c         2038 g         1390 t
ORIGIN      
        1 gttcctccag ccggaccgtg accttccgac gcggagcgaa agcgacgccg agaactgaca
       61 gagcgcggcc gatcaaaagg tgcacctcag tcgttgaaaa acccattgaa aacggcgtcc
      121 ggcatatcga cgataatcgc gccgagttgg gacgtgcaac ggaaacggaa ctgagacgcc
      181 gtcacgctct tgcccgcagt gccggtgacg gcctgcgcgt tgccaacggg cgtcttctcg
      241 ccctgctccc agaccttctg ccagccgtcg tcgaccttgg cctccatcac cagcgcatac
      301 ggcgctcgaa tagtaaagcg ctcgaccccc agcttcagcg cctgacaagc gacaggttgc
      361 gccagcgtca atgtcacctg ccgatcctcg ccgccctgga ttctcacacc cgtgccccgc
      421 gcgtaactgt agacctccgc atgaccggcc tcggcggtcc acacacccag acgggtttcg
      481 agccgctcga aaggcccttt tgccgcctgc ccgaacaggg gcggaacagg tgtggccttc
      541 tcaaccggag acggcgcata gaaccacggc accggcacgc gcgtgacccg cgaatgcgcg
      601 aggcaggaca tcgcgcagta accgagcagc acggcatcct gcaccgggtg gatcgcgata
      661 tagcagtaat gccctttcgg attgccttcc aacaccttcg tgttctgcca tgtcttgccg
      721 tcgtcttttg aaatcgccgt gctcagcggc acgcgtcgtc cgtgcaagcc tgcgggaatg
      781 ttcgcgtgat cattccagac cagcagcaaa tcaccggtcg agggcaggcg cttgattgag
      841 gcgggagaga caggagattt gatttccgaa gcgaccggcg cgctccacgt gtcgccgccg
      901 tcagaagacc aagcgagata ctggcagccg ccgctcgcgc ggatgaacat catcacgcgg
      961 ccgtccttca actcgaccac gccgggctcc tgcgtgatga tgcgcttacc agccgtgtca
     1021 tagccttttt gcacagtctt gctgcgacgc cacgtcttgc cgttatcgtc cgacaggtag
     1081 cacatgagca ccccctgcca atccgccttg gacgcgccct gcggccagtg caggcagacc
     1141 ggcaccacca accgcccgcc cttaagctgt atgacacggt cattgttcag cacataatag
     1201 ccgacctcat ccgtgataca catcaccggc gcaccccaag tctcaccctc gtcccgcgag
     1261 agccgcatca ccggacgaca atcctgctcg ttgttcttgg gcaggtaaaa gagcgcgatc
     1321 tcgccgcttt gcaggcgcaa caggctgacc gacatcacgt tcatgccgcc ctcgttcggc
     1381 acgatcaccc ggtcttgcgg cgtccatgtc cgaccgccgt cggatgaata gcgccccgct
     1441 aaatgggccg agtcgtgatc gcccccgcca cccgtgaaat gggtgtacac atacagaatg
     1501 cgcccgtcct tgagcgtgac gaacgccccc tcactgttac gcggattacc cggtcccgaa
     1561 ggcaattcca acgtgcgaac gggcacgtcc cgcacgccac atttcaagac acatccgatc
     1621 gtaaacattg acgccagaac gaaaccggcg attccagcca cggtgatttt tttcgtcata
     1681 cttccccttt ttccaatccc ttttcctcag tctccgcttc ttcctgcttg tcgtccgtac
     1741 cggtcccgaa ccagaaggag gtgctcggac tcagaccgcc ggagaagatc tcgcggtaag
     1801 cgtcagccgg aatctccgga gcacccgtga tcgtggcctt cccggtcagg atcagatcga
     1861 tcaggcccaa cgccaccggc tcgttgatgt ccatctggtc atgcagaaag aacggatcgc
     1921 cgaacacgcc ttggcgcgat ttcaactccg gagtctccgc cggaaacgcc tccaccgccg
     1981 cgaagagctg cacggactca ggatccccgc cgacatactg accgttcaca aaaatcagcg
     2041 caccgctgtc ttgggtcgcc gccgcgaccg gcgaactttt cccgccgacc gtattcaaga
     2101 aggcatagac aaccttatcc ggactgcccg ccacattgtt gacccagatg tcatacggtc
     2161 cgtgtccgcc atttaaccgg acctccagag gatcaatcgt gttctgtccc ccgcccgcac
     2221 cgatgatgtg ccccgctgaa acagtcagtg tgcctgccgt gatatccgtg ccggctccgg
     2281 tgccgtcgat gacagagttg tccgccatca gattcacatc cccggttccg gcattgatcc
     2341 cgctcagcgc cgggctcgtc gcggtctggt caacatcatc aagcgtcagc cgggtgaccg
     2401 acaccgcagg caccgacgcg atcgtcagat tttctttccc acagatcgcg atatttttac
     2461 cctccgccgc cacaccgttc gcgcgcaccg taatcggatt gaaggggcgc gtccccgaag
     2521 ttcccgcgcc cgtgtcaccg atattcccgc ctgcaaagaa cgacaccgtg tccgccacaa
     2581 tgttcacgac atcgttaggt atggcgaaac cgttcgcgtt gactatgatc atgcccatgt
     2641 tgtccgtgat atcgcctccg gcctccaccg cgacatcccc tgcccccgca tccagcaacc
     2701 cgtccaacac cacgtcgcca gacgccgcat agcggatgtt gccgcctgag gtctgcgaca
     2761 ccgccgccag actgatcgaa ccgccggccg tttccacgtc gatcgtgccg ccggccgtga
     2821 tgccgccgct ctgtatgatc ccccgtgagg ccagcagcgt cacatcgtcg cccgccgtca
     2881 ccgccgcgtt gagcgacaga ccgccgccct tggcgctgag aagcacgtcg cccgacgcgg
     2941 ccgtgaccgc ctgatcgacc gtcagaccgc ccgcggcacc cacaccgttc tcgatcttca
     3001 cgttctgccc ggccaccgcg cccgagagcg ccgcgccgtc cgccgtcgcg tccgaggtcg
     3061 agtccatctt cacgcggttc acgtcgaccg ccgccaccgt cccgacaagc agggcgtccg
     3121 cctcgctgag gtagacgctg cccgcgtcgc cgccctttgc cgcgaccgtc ccgacagcga
     3181 ggtcgagcgg gccgttgccc tcgccggact tctggcccac cgagccgccc gccacgagct
     3241 gcgcggcgga cgcctcgacg tcccttgcca tatcgccagc gtccgtgatg ccacctgccg
     3301 ccttcaccat caccgcgccg tccgccgccg tgcttttcag gcccgtgatc ttcgcgtcgc
     3361 cgcccttcgc ctcgtagcgc aggtcgtcgc ccgccgccgc cgttgccccg tccgccatga
     3421 cgatccccgc gcccgcctcg accaacacgt cgccagccgc cgccgtcacg tcaccggcag
     3481 cggcctgttc gatattcccg gaggccagca gcgtcacgtc gtcgcccgcc gccaccgccg
     3541 cgttcaggat cacgtcggag tccgcgccac ctcccgtcat cagcagcacg tcctccgcag
     3601 cccgcacgcc gacaccgtcc atttcagggc gcgggcccat tggctcgccg tcattcaccg
     3661 tcaaggagcc gttacgcaca cccaccttga ccgatccggc cgttgattcc agatcgctca
     3721 tgcgctcgat gtcccacgat ccgacgccaa ccgggctgaa catcacatag ttgatctgtt
     3781 cccccccatc cccaaccgtg ccgatcgtca aatccctgcc gtccggggct tggatgaaat
     3841 actggttgcc ctgactcagc gcggccaagg tccccacctg caggtcgacc ggaccgccgc
     3901 ccactccttc cgcctcgccg atgccgcccg ccggcacggt gaagaccgcg ccgcgcgcga
     3961 tgatgttgcg tgccccgccg tttccgtccg tcaccgaacc cgtgtcatcc cagacggaga
     4021 ccagacccca atccgtgtcc cggttccagt tctgctcatc gggatccgcc gagtccacca
     4081 gcgagtccgc gtcgttgtcc gatccgtcct ccgccacatc ccgcgcctcc cgggtccggg
     4141 cgtccaacag gcccaacgtg attccgcccg aggccgcata ccggatatgg ccgccgtccg
     4201 tttggcccag cgtcccgtcc gccataacga tggctccgtc tgccgcctcg acgtcgatcg
     4261 tgccgtcacc ggacacgacg aggaggtcgc ccttggccga acccgagacg ttctgcgcga
     4321 tgttccgcgc ggccagcaca ctgatagagc cggcatccga aaccaccgcc gcgttaagcg
     4381 tgatgtcccc gtcaacattg ccgttcatgc tccatgcgtc cagcagcacg tcctccgcag
     4441 cccgcacgcc gacaccgtcc atttcagggc gcgggcccat tggctcgccg tcattcaccg
     4501 tcaaggagcc gttacgcaca cccaccttga ccgatccggc cgttgattcc agatcgctca
     4561 tgcgctcgat gtcccacgat ccgacgccaa ccgggctgaa catcacatag ttgatctgtt
     4621 cccccccatc cccaaccgtg ccgatcgtca aatccctgcc gtccggggct tggatgaaat
     4681 actggttgcc ctgactcagc gcggccaagg tccccacctg caggtcgacc ggaccgccgc
     4741 ccactccttc cgcctcgccg atgccgcccg ccggcacggt gaagaccgcg ccgcgcgcga
     4801 tgatgttgcg tgccccgccg tttccgtccg tcaccgaacc cgtgtcatcc cagacggaga
     4861 ccagacccca atccgtgtcc cggttccagt tctgctcatc gggatccgcc gagtccacca
     4921 gcgagtccgc gtcgttgtcc gatccgtcct ccgccacatc ccgcgcctcc cgggtccggg
     4981 cgtccaacag gcccaacgtg attccgcccg aggccgcata ccggatatgg ccgccgtccg
     5041 tttggcccag cgtcccgtcc gccataacga tggctccgtc tgccgcctcg acgtcgatcg
     5101 tgccgtcacc ggacacgacg aggaggtcgc ccttggccga acccgagacg ttctgcgcga
     5161 tgttccgcgc ggccagcaca ctgatagagc cggcatccga aaccaccgcc gcgttaagcg
     5221 tgacgtcccc gtcaacattg ccgttcatgc tccatgcgtc cagcaagatg ttccccgctg
     5281 cccgcacgcc gaccccgtcc atatccgagg actgcgcgtc cgcgagtccg tcgctcactg
     5341 tgatcgcccc ccactccgtg gccaggcgga tgtcaccact ctcggattgc agatcgctct
     5401 tggcatcgag ataaacttca gtcagcgagg aatccagccc gacgcggttc acaaaggcgc
     5461 gctcatccac accggccgtt tccggatcat ccggggttcc caccgtaccg accgtcacat
     5521 cgccaccggc cgcaacctga atgaagttct gtgacccgta actgtgcgcg gccagcacat
     5581 ccacatcggt caacaccggt ccgttgccga ttccgggtat ggcaccgatg tcccccccct
     5641 gcgaactgaa ctgcgccccg gacgcggtca cattcaccgc gccgcctgcg cttacgatcc
     5701 cgcgggatgc gtccacggcc accaaaccgt cacccgcatt cagatgcccc atcgtgacag
     5761 ccccgtttga cgaaaaataa cggatgttgt cacccgccga accgccgctc gcgctcgtgg
     5821 tgtccggtgc catcacaatg ttaccggtgg tccgcacatc gatcgtgccg cccgccgtga
     5881 tatccgcgtt ctgccggaag gcgtcactcg ccagaaccgt cacgttgtcc gccgccgccc
     5941 cggctgccgc gttcaggatc acgtcggagc ccgcaccgtt cgccgccagc agcaaatccc
     6001 ctgctgtggc cgtcacagtc tgtcccaccg tcaaggtgcc gttgatcgtc tcgaccttgg
     6061 cgttttgccc ggctgtgacc ccggaaaccg taccgaccgt gccgaccgtc agcatgtcgg
     6121 tttcgtagag gtaaacgctt ccgttggccg aggacgccgc caccgtcttg accgtaagat
     6181 ccagcggatt ccctgcggca ccgatatcgc cttgcgcggt catccccagc tgatccgcca
     6241 caacgttgac cgtgcgccca gcgttctgcg cgaatccctg tgcacccacc gtcacggtat
     6301 ccgcctgcag atccgtgatc gcgcccgtac ccgcatccag caccacatcc gccgccgtgc
     6361 ccgcgcccgc cgagatgact ccaaccgcaa tgccttcacc cgtcagcaca atgtcgttcg
     6421 catcgttgtc cgtcaagccc gccacatccg tcgccgtgat ctgccccccc gccgtcacgg
     6481 taatcgcgcc gttgaccgtg tccacatcag tcaggatgat gtcgtctgtg tcggccagtt
     6541 tcagcgagag ccctgcatgc gccgtcacgc cgtcctgaaa atcattcgcc gcattatcaa
     6601 gcgtgaccgc gcccgaggcc gtaagttcgg cagtacccgc gatttcaagc gcaccgacca
     6661 gatcttgcgc aataccctga ccttccaccg ctgcggtaac gtcaccgctg aacgtgccgc
     6721 ccaactgcat gtcgttggcc gcgtctcctg ccagcgtgat ggaaatggtg ccgccgttaa
     6781 ccgtgtcgat tatgatcgaa tcacccgccg ccgccaacac ggcctgcggt gtcgtgagca
     6841 cgccgttcat cgaaacttcg ccgcccagca gagccgccaa ggtcccggcg gcgatactcg
     6901 caccgctgtc cacctgaacc ggaccggtgc cgctgaccgt accatcaaaa ttcatgttgc
     6961 cggccagaaa atcctcattg gaaatgtcca tggtcgcggc cacgaacgcc gcgccgacat
     7021 tgattctggc cccgttctgg atgaagacgc cgttggggtc caccacccac acgccgccgt
     7081 tggcattgat ctgccccgcg atctgcgtgc cgtccatact cgaggtgata cggtttaggg
     7141 tcaccgctgc ggcatccggc tggctgaaat tcgcgatctc gccggccgct atgtcgaaat
     7201 cggtccagtt gatgatgccc gcctgctcgg cctgattgat ggtggtcaca ttcccgaccg
     7261 tgccgccggt gatgcctccg gcgaccggtg tggcgttcgc ccaatccaag gcgtgcaccg
     7321 gcatactggt catcaggaca ctgaaagccg caaccgcgga caagacgcgc cgtaccggtt
     7381 ttcctgcgaa caggatggta tgtcgtgtat tcatggtttg tcctttgcga tcagagagtc
     7441 cttgatgaaa tccgttaaaa ctgaagctgc caactcaggt agtattccga agagtcgtcg
     7501 tctccggagg ccgatgccac cggcacgccg acgtcgaaac gcaactggct gtaccgcatc
     7561 acggcgagac gcacgcccgc gcccacactc aggagggttt cgttgtcatc gac
//
LOCUS       DAOSRB010000136         7598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9062_length_7598_cov_8.088029, whole genome shotgun sequence.
ACCESSION   DAOSRB010000136 DAOSRB010000000
VERSION     DAOSRB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9062_length_7598_cov_8.088029"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            567..2102
                     /locus_tag="PLZ74_07910"
     CDS             567..2102
                     /locus_tag="PLZ74_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOR98285.1"
                     /translation="MSYYLGIDCSTQGMKALVIDPAASRVVAGASVRFTTDLPEFGCP
                     DGVLPHPDPLIRHADPLLWVAGLERTLKRLQDTGVEMERIAAVGGDGQQHGSVYLNRR
                     FGEVLKRLSPDTPLAGQIAPALSRPTSPIWMDSATSQACRELDARFGARLRTETGSPA
                     IERFTGPQIRHFAQRDPERYADTARIHLVSSFLCSLLIGRDAPVDAGDGAGMNLLNLR
                     TLSWDPEITEFTAPGLAEKLPTIGSGVAGGLHACFARFGLRSGIPVVVWTGDNPASLV
                     GTGAWGAGAAVVSLGTSDTFFAALDRFHTDPEGFGHVFGNPAGGFMSLTCFKNGSLAR
                     DRVREDAGVDWAFFDETAFDLTPPGNQGRLALPWFVPEITPPVLTPGLRTNFAFDAAP
                     PEVRIRAVVEAQALALRSHSLWIGAFDTLRVTGGASKSPGIRQTLADVFQSRVESIAV
                     SDSAALGGAMLAAHAADGVPLDVLTRGLTAVTAVCEPRDDVSEVYEALLPELHALERG
                     DPI"
     gene            complement(2129..4528)
                     /locus_tag="PLZ74_07915"
     CDS             complement(2129..4528)
                     /locus_tag="PLZ74_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOR98286.1"
                     /translation="MRIPPPTRRLCLAFALLSAGSVFAADYAYQPAPITRVRLLDGFW
                     LSRAETNRLVTVWANFGKCEETGRIANFARAGRLESGPFQGTAWNDSDVFKVIEGAAH
                     TLATHPDPKLDGYLDRLIVRIAAAQEPDGYLYTARTLGATNEMTGPARWSNVQSSHEL
                     YNLGHLFEAAVAHHAATGKRMLLDVACKSADLVCRVFGPGPEQRKDVPGHEEIEIGLC
                     KLYRATGERRYLDLATFFVAMRGRKDLRGRVFGPTYQDHLPVLEQSEAVGHAVRAGYL
                     YAGIADVAALTGDTAYLAAIDRLWENTVSKKLHLSGGIGARRSGEAFGENYELPNETA
                     YLETCAAIANALWNQRMFLLHGDARYIDVLERVIYNGFLSGISLSGDTFFYPNPLESR
                     GGYARSKWFGCSCCPVNIVRFIPQIATFAYAQRDATIFVNLFLASEARLTTPAGDVTL
                     TQKTDYPWRGKVRIEVAPSRDGLAFPLRIRVPGWATGQPVPSDLYTQTRPATPEAVSL
                     SLNESPVPVRIESGYVTLDRAWKKGDAVTLTLAMPVRRIRAHDAVRDNRGRLAVERGP
                     LLYCAEGVDNNGRARSLTLAPEAVFSEASLSLSEHTVTALQTTGSRVTANLRGKRTVQ
                     PATIILIPYYAWCHRGAGEMQTWFPTGVAEADPMSDFHITASHCYVKDSPEAAIDGVL
                     PKHSHDNTINRMTWWPRKGSEEWLQVDFPEEESVCGSQVYWFDDTGKGACRLPVSWRI
                     QYADADGGWRDVATEYPVAEDRFCEARFSQPVRARRFRLSVRLQTDFSGGVLEWRLLP
                     E"
     gene            complement(4534..5994)
                     /locus_tag="PLZ74_07920"
     CDS             complement(4534..5994)
                     /locus_tag="PLZ74_07920"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOR98287.1"
                     /translation="MCSRSFVLFCFLFGGPVCAMSATEPPIRIVFPAEGARLAYTDRV
                     FVIGAVRPADVPLTLDERPITPHRTGGFLHMAPVSPGTNTLIFNAGGTVIRHTFRIDP
                     PPQSWDGCSLRAQAPLQPLGLCTGETVRLACLAPADRTVFAAVGERTLALVPQPGHAT
                     LYVAKLAFDVPVADVPVTFFATGLLDAAAAPLTARADWPALRTTGPLFAMRARSEPGG
                     GETVAFLPPDLTVRGAGFVGAHTRFWLAGERRFMESRHLTSLAPEHPLPPCDLPIPDL
                     TAGFTLNPPVGRAPDSILIVLDPGHGGASTGAVGPTGLTEKEANLIQAHRVAATLRKA
                     GFRVLLTREDDKDLDLYARVRLAYERKADAFISIHYNSCGPAGNPSESRHIATYAWNA
                     IGLRLARALHPHLAAASPAADGGVRTASFAVCRNPVVPACLLELDFITCPLGEESLQQ
                     PDQRERVAHAVLAGLRDWLTPSPRTESANNATPNKE"
     gene            6300..7181
                     /locus_tag="PLZ74_07925"
     CDS             6300..7181
                     /locus_tag="PLZ74_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOR98288.1"
                     /translation="MTKYMLIATVGWYALSGVHAADLRCPLPGKWELDTSVSDEFDAD
                     SLDKTKWWDYAPHWPGRPGVYRTLARNVSQREGCLLLTTDKVPDSEIPYEARMDGMQG
                     YTCALVKSRKKVIYGYFEARIKATPAAIRNAFWLYDPLSDDLNRKYSPGDVSEEIDIC
                     EILGKHQPGDKNQSYVTSHYTHHYLTPYYEGICNNLNVPLGVMRELAFCPSADYHVYG
                     LLWTKDELVWYVDNVESARMPTGSFAQGGFRRPLHVVLDTEVNDWSGAKVSELDARTL
                     PATAKVDWFRRWVPISK"
BASE COUNT         1449 a         2145 c         2537 g         1467 t
ORIGIN      
        1 acgtattgag atgcgggcgg agcccgcaat ctcaaaggct gtaagactgt gaggctgtaa
       61 gactgtgagg cggttgtgtt ctgtggtgaa acaatcgctc ccgctcccga tagcgatccc
      121 gaccccgatt tggatgaaac cctgaaacca tgaaaccaga aaccctgaaa ccatgaaacc
      181 ctgaaaccca agagagttga aggttcaact gctcttttta ggataaatgg acaggattac
      241 aggattaaca tgattttttt aatcctgtca tcctgtcata aaaacgtatc ccagagtttt
      301 ctcgcggctc cggcgcgcgg cgggcttgcc tataacttac atcaaagtta tcctcattta
      361 catttccgtg cgaagcgcgg gcggcggggt aaccccccaa acggcactta ccgaacaccg
      421 cgactggagg cggttgagtc gcattgcgca ggatctgggc ttctgcacct cacagtacta
      481 catccggccg cttgtttacg gttgcgcgaa cgagtcggct cgggtacgct gaacaccggt
      541 cgggtttgat tacagggagg ttcggcatgt cgtattatct tggaatcgac tgtagtacac
      601 agggtatgaa ggcgttggtg atcgatccgg ccgcaagccg cgtggtggcc ggtgcgtccg
      661 tccggttcac cacggacctg cctgagttcg gatgccctga cggcgtattg ccgcaccccg
      721 atccgttgat ccggcatgcc gatccgttgt tgtgggtcgc ggggttggaa cggacgctga
      781 agcggcttca ggatacgggc gtggagatgg aacgtatcgc ggcggtgggc ggggacggac
      841 agcagcacgg ttcggtctac ctcaaccgcc ggttcggcga ggtgctcaaa cggctctcgc
      901 ccgacacgcc gctggccggg cagatcgctc ccgcgttgtc ccggcccacg agtccgatct
      961 ggatggatag cgccacctca caggcatgcc gggagttgga cgcacggttc ggtgcgcgtc
     1021 tgcggaccga aaccgggtcg cccgccattg agcgtttcac cggtccgcag atccggcact
     1081 tcgcccagcg cgatcccgag cggtatgccg acactgcgcg catccatctc gtcagttcgt
     1141 ttctttgttc ccttctgatc gggagagacg cgccggtgga tgccggcgac ggtgcgggca
     1201 tgaacctgct caatctgcgg accctctcgt gggatcccga gatcacggaa ttcaccgctc
     1261 cgggtctggc cgaaaagttg ccgacgatcg gcagcggcgt ggcgggcggt ctgcatgcct
     1321 gctttgcccg cttcggcctc cggtccggca tcccggtggt tgtgtggacc ggcgataatc
     1381 ccgccagtct ggtgggcacc ggcgcgtggg gcgcgggcgc ggcggtggtg agtctcggca
     1441 ccagcgacac ctttttcgct gcactcgacc ggttccacac cgacccggag gggttcggac
     1501 atgttttcgg caatccggcg ggtggattca tgagcctgac ctgtttcaag aatggctcgc
     1561 tggcgcgcga tcgggtgcgc gaagacgcag gcgtggattg ggcattcttc gatgagacgg
     1621 catttgatct gactccgccc ggcaatcagg ggcgactggc gttgccgtgg tttgtgccgg
     1681 agatcacgcc gccggtgttg acacccggcc tgcggacgaa ctttgcgttc gacgccgcgc
     1741 cgccggaggt gcgtatccgt gcggtggtgg aggcgcaagc cttggccctg cgcagccatt
     1801 ccttgtggat cggcgcgttt gacacgttac gcgtcaccgg cggtgcctcg aagagtcccg
     1861 ggatccgcca gaccttggcc gacgtgttcc agtcgcgtgt ggagagcatc gcggtgtccg
     1921 attccgcagc gttgggcggc gcgatgctgg cggcgcatgc cgcggatggc gtgccgctcg
     1981 atgttctgac gcgcggtctg accgccgtga cggcggtgtg cgaaccccgc gacgacgttt
     2041 ccgaagtcta tgaggcgttg ctgcccgaac tccacgcact ggagcgcggc gacccgatct
     2101 gaagagagac cggggtcggc acggcaggct attcgggaag cagccgccat tcgagaacgc
     2161 cgccggagaa atcggtttgg agccgcacgc tcagacggaa ccggcgggca cggaccggtt
     2221 gcgaaaaacg cgcctcgcag aaccggtctt ccgcaaccgg gtattcggtc gccacatcgc
     2281 gccagccgcc gtccgcgtcc gcgtattgga tgcgccacga gacgggcagg cggcatgcgc
     2341 ctttgccggt atcgtcaaac cagtagacct gacttccgca gacggactcc tcctcaggaa
     2401 aatcgacctg cagccactct tcgcttcctt tacgcggcca ccaggtcatg cggttgatcg
     2461 tgttgtcatg ggaatgtttg ggcagcaccc cgtctatggc cgcttcaggc gaatccttca
     2521 catagcagtg cgaggcggtg atgtggaagt ccgacatcgg atccgcctcg gccacaccgg
     2581 tcgggaacca ggtctgcatc tcgcccgcgc cacggtggca ccaggcatag taggggatca
     2641 ggattatcgt tgcgggttgc acggtccgtt taccccgcag gttcgcggtc acgcggcttc
     2701 cggtggtctg cagggcggtc accgtgtgtt cggagaggct aagggacgct tcagaaaaga
     2761 ccgcttccgg cgcgagcgtg agcgatcgtg cgcggccgtt gttatccaca ccttcggcgc
     2821 agtacagcag cggaccgcgt tcgaccgcga gccgaccgcg gttgtcacgc acggcgtcat
     2881 gagcccggat ccggcgtacg ggcatggcga gcgtcagggt cacggcatcg ccttttttcc
     2941 atgcgcggtc gagggtgacg tacccgctct cgatccgcac cggcacgggc gattcgttca
     3001 gggagaggga aaccgcttcc ggcgtggcgg gccgggtctg tgtatagaga tcggagggta
     3061 cgggctgccc cgtggcccat cccggcacgc ggatccggag cgggaaggcg agcccgtcac
     3121 gggagggggc aacctcaatc cgtaccttgc cccgccacgg gtagtcggtc ttttgcgtca
     3181 gcgtcacgtc gccggctgga gtcgtcagcc gggcctcgct ggcgaggaag agattcacga
     3241 agatggtggc gtcgcgttgc gcgtaggcga acgtggcgat ctgcgggatg aagcgcacga
     3301 tattcacggg acagcaggag cagccgaacc atttggagcg cgcgtagccg ccgcggctct
     3361 cgagcgggtt gggatagaag aacgtgtcgc cggaaaggct gatacccgag aggaacccgt
     3421 tgtagatcac gcgctccaga acatcgatat atcgggcgtc gccgtgcagc agaaacatgc
     3481 gctggttcca gagggcgttg gcgatagcgg cgcaggtctc cagataggcg gtctcgttcg
     3541 gcagttcgta gttctcaccg aacgcctcgc cactgcggcg cgcaccgata ccgccggaga
     3601 gatgcagctt ttttgaaacc gtgttctccc acaggcggtc gatcgcggcc agataggccg
     3661 tgtcgccggt caacgccgca acgtcggcaa tgcccgcata gaggtaaccg gcccgtacgg
     3721 cgtgccctac ggcttcgctc tgttcgagaa ccggcaggtg gtcctgatag gtcggtccga
     3781 aaacccgtcc gcgcaagtcc ttgcggccgc gcatggcgac gaaaaatgtg gcgagatcga
     3841 gatagcggcg ttcgccggtg gcgcgataga gtttgcagag accgatttcg atctcctcat
     3901 ggcccggcac atctttgcgt tgctccggtc ctggaccgaa cacccggcag acgagatccg
     3961 cgcttttgca cgccacgtcc agcaacatcc gtttgccggt tgcggcgtga tgcgcgaccg
     4021 cggcttcgaa gaggtgcccg aggttataga gttcgtggct ggactgcacg ttactccagc
     4081 gcgcgggacc tgtcatttca tttgtggctc cgagcgtacg cgcggtgtag agatatccgt
     4141 cgggctcctg cgcggctgcg atgcggacga tcagccggtc gagatatccg tccaacttcg
     4201 ggtcgggatg tgtggccaga gtatgggccg caccctcgat gaccttgaag acatcagaat
     4261 cattccaagc cgtgccttga aagggaccgg actcgagtcg gcccgcgcgc gcgaagttcg
     4321 cgatacgtcc ggtctcctcg cacttgccga aattggccca gaccgtaacc agccggttgg
     4381 tttccgcgcg cgacagccag aaaccgtcca gcagcctcac tcgcgtgatc ggcgcgggct
     4441 ggtaggcgta gtccgccgca aacacggagc ctgcggacag gagtgcgaaa gccagacaaa
     4501 ggcgacgggt ggggggtgga atgcgcatcg gtttcactcc ttgtttggtg ttgcgttatt
     4561 ggccgattcg gtacgtggcg aaggcgtgag ccagtcgcgc agaccggcca gaaccgcgtg
     4621 ggccacgcgt tcgcgttgat cgggttgttg aagcgactcc tcgccgaggg ggcaggtgat
     4681 gaaatcgagt tccagcaggc aggcggggac gacgggattg cggcaaaccg cgaagctggc
     4741 ggtgcgcaca ccaccgtcag ccgcagggga cgccgcagcc agatgcgggt ggagggcccg
     4801 ggccagacgg aggccaatcg cattccaggc gtaggtggcg atgtgtcggc tttcggaggg
     4861 gttgcccgca ggtccgcagg agttataatg gatcgagatg aaggcgtcag cctttcgctc
     4921 ataggccagc cgcacacgcg catacaggtc taaatccttg tcatcctcac gcgtcagcaa
     4981 cacgcggaat ccggccttgc ggagcgtggc ggccacccgg tgcgcctgaa tcagattggc
     5041 ctctttttct gtcagcccgg tagggcctac cgcgccggtc gaagcgccgc cgtgtccggg
     5101 gtccagcaca atcaaaatgg agtctggcgc gcggcccacg ggcgggttca aggtgaatcc
     5161 ggccgtgaga tcgggaatcg ggaggtcgca gggcggtagg ggatgttccg gcgcgaggga
     5221 agtgagatga cgcgactcca tgaaccggcg ttcgccggcc agccagaaac gggtgtgtgc
     5281 gccaacgaac ccggcgcccc gcacggtcag atcaggaggc aaaaaggcca cggtctcgcc
     5341 gccgcccggt tcgctacgcg cgcgcatcgc gaagaggggg ccggttgtgc gtagggccgg
     5401 ccagtcggcg cgtgcggtga gcggggctgc tgcggcgtcc aacaacccgg tggcgaaaaa
     5461 ggtcaccggc acgtcggcga ccggcacgtc aaacgcgagc ttggccacgt aaagcgtggc
     5521 gtggccgggt tgcggtacga gtgcgagggt ccgttcgccc accgcggcga agaccgtgcg
     5581 gtccgctggc gcgaggcagg cgagccggac cgtttcgccg gtacagagcc ccagcggttg
     5641 gagtggagcc tgcgcgcgta gggaacagcc atcccaactc tggggcggcg gatcgattcg
     5701 gaaggtgtga cggatcacgg tccctcccgc gttgaagatc agggtgttgg tgccggggga
     5761 gaccggagcc atgtgcagga atccgccggt gcggtgcggt gtgatcgggc gttcgtcgag
     5821 cgtcagcggc acgtcggcag gccgcaccgc gccgatcaca aacacgcggt ccgtgtatgc
     5881 cagtcgcgcg ccttcagccg ggaagacgat gcggatcggg ggttccgtgg cggacatcgc
     5941 gcatacgggg cctccgaaca ggaagcagaa gaggacgaaa ctccgggaac acatgcccgg
     6001 gagcgtagca aagccgtaac ggaatgtaaa accgggtgtt ttcaatttac acatgtgggg
     6061 cggaatttga aaaagcgatt caggcgttga ggtcgagaaa atgaaaacct tttttctatg
     6121 tggtaaaaaa ttcgtttttc ctgaatttgt ttcgtaatcg atcctgtttg cttctgaaga
     6181 ttgtgatacc atatttaccc acgccgattg tccgcgccaa ggtcgagcgg gctcagaaac
     6241 ttcttctcaa gaaggagtgt ctacgcatgc aaaggtgaga gaacaggagg tacaggcaaa
     6301 tgacgaaata catgttgatc gcaacggtgg gttggtacgc gttgtccggg gttcatgcgg
     6361 cagatcttcg ctgcccgttg cccgggaaat gggaattgga tacaagcgta agcgatgagt
     6421 ttgacgcgga ttcgctcgac aagacgaaat ggtgggatta tgcgccccat tggccgggtc
     6481 ggccgggtgt ctatcggacg ttggctcgga atgtttcgca gcgggaagga tgccttttgt
     6541 tgacgaccga taaagtgccg gattcagaga tcccgtatga agcccggatg gatggcatgc
     6601 aggggtatac gtgcgcactt gtgaaaagcc gaaagaaagt gatatacggc tattttgaag
     6661 cgcgcatcaa ggcaacgcca gccgcgatac gaaatgcatt ctggctttat gacccgctaa
     6721 gtgatgattt gaacagaaag tactcgccag gcgatgtgtc ggaggagatc gacatctgtg
     6781 aaattctcgg caagcaccag ccaggcgaca aaaaccagtc gtatgttacg tcacattaca
     6841 ctcatcatta tctgacgccg tattatgagg gcatctgtaa taacctgaac gtgccgctcg
     6901 gcgtcatgcg cgagctggcg ttctgcccta gtgccgatta tcacgtttac gggctccttt
     6961 ggacgaaaga cgaacttgtg tggtatgtgg acaatgtgga aagcgcccgt atgcccacgg
     7021 ggagctttgc ccaaggcgga tttcggcgtc cattgcacgt cgtcctcgac accgaagtca
     7081 acgactggtc gggtgcaaaa gtgtctgaac ttgatgccag gacattgccg gcgacggcaa
     7141 aagtcgactg gtttcgcaga tgggtgccga tcagcaaatg agtccctcga agaggcgcta
     7201 aaatgaggca gatgaaaaca agttgtttct cgcgcaaaga cacgaagagg aatagattgt
     7261 catgggaacc cctttgtgtc cttgtgtgat tctaaatggg ttgcaggctc cacgttggtt
     7321 ttaggaggga gggaaagagg gggaggcgga ttattgcgat cagggggctg aacctaaaaa
     7381 gagcagttga accttcaact ctctttggtt tctggtttca gggtttcggg gtttcggggt
     7441 ttcatccaaa tcggggtcgg gatcgctatc ggtatcggta tcggtatcgg tatcgggatc
     7501 gggaacgtcg acccgcgacc cgcgacattc gacccgcgac gatcgacagg gacgcccgcg
     7561 tttccgacac cgcaggtcgc tcgtcgcggg gcgacata
//
LOCUS       DAOSRB010000137         7575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9095_length_7575_cov_6.116090, whole genome shotgun sequence.
ACCESSION   DAOSRB010000137 DAOSRB010000000
VERSION     DAOSRB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9095_length_7575_cov_6.116090"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..1729
                     /locus_tag="PLZ74_07930"
     CDS             452..1729
                     /locus_tag="PLZ74_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOR98289.1"
                     /translation="MQLSFHLDDQEEEPPQGASPLFSLSVPFTDKKPELTHLPEPPVK
                     SRGLRMSPCCRAFRNRFFADATDLQWSDWRWQIQHRIRDLETVRRMIRLTAAEEKALE
                     LGGKMLPMAVTPHYMGLISPDDPEQAIRRAMIPTVYELRHCPGEAEDPLGEEHQSPIP
                     GLVHRYPDRVLLLATDFCSAYCRYCTRSRVVGHGAITPSTRRLKMIFEYIRERPQIRD
                     VLVSGGDPLTLSDERLEFILSNLRAIPHVEILRLGTKVPAVLPQRVTPRLTRMLRKYH
                     PLWMSLHFMHPDECGVETSRACGMLADAGIPLGSQTVLLRGMNDDPDVMRLLMHKLMK
                     MRVKPYYIYQCDPITGSEHFRTPVAKGVEIIRSLRGHTSGYAVPTYVIDAPGGGGKIP
                     VAPEYTAGRDAEGNLNLINYEGGSYRYPDFIPG"
     gene            1765..2754
                     /locus_tag="PLZ74_07935"
     CDS             1765..2754
                     /locus_tag="PLZ74_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOR98290.1"
                     /translation="MRIGLTYDLRADYLALGLSLEETAEFDKEETIAGIEDALRALGH
                     TTERIGHARALIRRLEAGERWNLVFNICEGLRGVAREAQVPAILELYEVPYVFSDPLV
                     LALTLHKGMTKRVVRDAGIPTSDFLVYETDEDLAELRFGAPFFVKPVAEGTGKGCSAK
                     SVVRERAALAAVCDDLVARFRQPALIEPYLPGREFTVGITGTGRQAAVAGSMEIVLLP
                     HAEAGGYSFDNKGNYEDRVVYPATDPARDPVAAMAERIALDAWRALGCRDGGRVDLRC
                     DASGRPQFLEVNPLAGLNPKDSDLPILARQCGITYTELIGRILDSAIQRQAGG"
     gene            2949..3311
                     /locus_tag="PLZ74_07940"
     CDS             2949..3311
                     /locus_tag="PLZ74_07940"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOR98291.1"
                     /translation="MRIKAKEPVKKVVKAVAKGKDKPVQTKTRRTAPKTQRILFSVRA
                     EVGSQVSLAGSFNNWDPTAKPMTDKKGDGVYTVTVSLAPGTYEYKFVIDGTWCADPDC
                     ADWVQNEHGTLNSVKYVR"
     gene            3356..4399
                     /gene="pdxA"
                     /locus_tag="PLZ74_07945"
     CDS             3356..4399
                     /gene="pdxA"
                     /locus_tag="PLZ74_07945"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOR98292.1"
                     /translation="MADARIRCALTLGDGAGVGPELALRALADEELSRTCQLLVYGNR
                     TLLERVAVASGIPFAATIKILDGAEGPLPQDGHVLFDFPFAEADSITPGTVQAACGRQ
                     AYEWVRRATLDISRNQVAHALVTAPLNKSALHAAGVQAVGHTEILSELTETPDPRMLF
                     YAPAFCVCLATIHEPLARVSELLTVSSLLRTLRLTHEACRMFGTPAPRLGVLALNPHA
                     GENGLLGTEERRVLMPAIETARAEGIHASGPLVADTAFTWLLSTPPQSAPYDAYVAMY
                     HDQGLIPFKIAAFDHGVNVTLGLPILRTSPDHGTAFDLAWQGTASPSSLFSAIRLAVR
                     MARNRMPPCPSVS"
     gene            complement(4408..4824)
                     /locus_tag="PLZ74_07950"
     CDS             complement(4408..4824)
                     /locus_tag="PLZ74_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOR98293.1"
                     /translation="MKSYRKELWFKTDHRREYRNITPEVEACLAESGIQEGFCLVNAM
                     HITASVFVNDDEAGLHADFERWLERIAPEKPHSQYAHNGAEDNADAHLKRQVMGREVV
                     VAVTRGKLDFGPWEQIFYGEFDGLRSKRVLVKIIGE"
     gene            complement(4821..6773)
                     /locus_tag="PLZ74_07955"
     CDS             complement(4821..6773)
                     /locus_tag="PLZ74_07955"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98294.1"
                     /translation="MTVNWVTEWMHSPEGFVVRMLTDGVKYAGAFFGALAVSLALTPL
                     CREAARKIGMMDLPDARRINKQPVPRGGGLAIIVSFHLVLGVMVWGLRSPISSEFTLY
                     WQGHFLGASALLAVIGFLDDFRGLRPVVKLAGQIIVAVILYLSGVHVGGIIVAFPPWL
                     DFLVTTLWIVGAINAFNLIDGMDGLATGLALIASMGLIGALLFTGKSAATLPYLVLAG
                     ACLGFLRYNFHPASVFLGDTGSMFLGLCIATFPLMTGSRKELAASLGMPLLVMGVPLF
                     DTLLAIWRRSVRACLPSGLVEVGKRVRVMQPDQDHVHHRLLRQTMNQKTAAIILYVIS
                     GVLVLIGLVGTMLKGRAPGLFLIAFVVAVYIVVRHLERVELWDTGRLLSGRHGMMRQG
                     LLIPLYIVWDVFALCGTWMVVRWESGLSLNRAAVLSELPVFIVPVFMALVATKTYARV
                     WGRAQIRDFVVLGLSVLAGTAIGAGLIWLLNDYGCGMIRFAVRFGTLAIFPIAGVRAW
                     RECVRGVMQMLERRVLLENPSALRFLAYGGGLRFRAYLRELMERPGNHDRVIIGILDD
                     EVNLKGRIIAGYPVLGDFETVRSYAADNSIKGVIITCLLPEAKLRWVTAELEAMGLVV
                     SVWACGERVLANPKGLVVREIGKGET"
     gene            complement(6783..>7575)
                     /locus_tag="PLZ74_07960"
     CDS             complement(6783..>7575)
                     /locus_tag="PLZ74_07960"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HOR98295.1"
                     /translation="ECGTQNAECGTQNAEQRSGGGMWIVCGVCAAVLAVFAANRAMPP
                     AVYDDASFIAKTLPERVGVFTGWKPLFCHNPQCLQGGEEPAGATEAEPAQRCPGCGGA
                     LFPISLGEASELPRDTVILKRTYRSVDGVSYAVSVVLGGRSRSSIHRAELCLPAQGYV
                     MLDAERLDLRVAGRAPLRVRSIRAARPGGGEMQLLYWFMSRERESCSHMERIWLDVWD
                     RSIHNRINRWVMVAVNVASGLATPEQVEAFEAFLSELYPALMVGR"
BASE COUNT         1632 a         2546 c         2038 g         1359 t
ORIGIN      
        1 gatcccgata gcgatcccga ccccgatttg gatgaaaccc tgaaaccaga aacccagaaa
       61 ccctgaaacc aaagagcgtt gctttcaacg ctctttttaa ggcgtccgcg ggcgtccctg
      121 tcgatcgtcg cgggtcgaat gtcgcgggtc gcgggtcgac gttcccgatc ccgatcccga
      181 tagcgatccc gaccccgatt tggatgaaac ccagaaacca tgaaaccaga aaccctgaaa
      241 ccagaaacca gaaaccctga aaccctgaaa ccagaaaccc ttaccggtca caaaaaaaac
      301 ggatccgcaa tctaagcatg cttgacaagc ttcccaaaca agcgtattag gaaaccacaa
      361 agaggctcct tgcgtcgcgc tgtgacgtga cgagggttat aggcctattt ttgtttagga
      421 gtttgctgtg tacggtgtgc ctaagcacgc gatgcagttg agctttcacc tggatgacca
      481 ggaagaggaa cctccacaag gagcgtcgcc tcttttttcc ttatctgtcc cgtttacgga
      541 caaaaaaccg gaattgacgc atcttcccga accgccggtc aaaagtcggg ggctccggat
      601 gagtccctgc tgccgcgcgt ttcgcaaccg gtttttcgcc gatgcgaccg acctccagtg
      661 gtcggactgg cgttggcaga tccaacaccg gattcgggac ctcgaaaccg tacgccgcat
      721 gatccgcctt accgccgcag aggagaaggc gctggagttg ggcggcaaga tgttgccgat
      781 ggccgtcacg ccccactata tggggctgat ctcgccggac gatccggagc aggcgatccg
      841 gcgcgccatg atccccacgg tgtatgagct gcggcattgc cccggcgagg cggaggatcc
      901 gctgggcgag gagcatcaga gcccgatccc cgggctggtt caccggtatc ccgaccgggt
      961 gctgctgttg gcgaccgact tctgttcagc gtactgccgg tactgcacac gctcccgagt
     1021 ggtcgggcac ggcgcgatca cacccagtac ccggcggctc aagatgattt tcgagtatat
     1081 ccgcgagcgt cctcagattc gcgatgtcct ggtttcaggg ggtgatccgc tgaccttgag
     1141 cgatgagcgc ctcgaattca tcctttcgaa tctgcgggcg atcccgcatg tcgagatcct
     1201 ccgcctcggc accaaggtgc ccgcagtcct gccgcagcgt gtcacgccgc ggctcacacg
     1261 catgttgcgc aaataccacc cgctctggat gagtctgcat ttcatgcacc ccgacgagtg
     1321 cggggttgag accagtagag cctgcggaat gctggccgac gccgggatcc cgctcggttc
     1381 gcagacggtt ctgctccggg gaatgaatga tgatcccgac gtgatgcgcc ttctgatgca
     1441 caaactcatg aagatgcgcg tcaaaccgta ttacatctac cagtgtgatc cgatcaccgg
     1501 ttcggagcat ttccgcacgc cggtggcgaa aggtgtggag attatccgca gcctgcgtgg
     1561 ccacaccagt gggtatgcgg tgccgaccta cgtgatcgac gcgccgggcg ggggagggaa
     1621 gatcccggtc gcgccagaat acacggcggg ccgggatgcc gaggggaacc tgaacctgat
     1681 caattatgag ggcggttcct accggtatcc cgatttcatt ccaggttgag atacctgttg
     1741 gacaggaaat acggacatgc ccttatgcgt attggcctga catatgacct gcgcgcggat
     1801 tatcttgcct tgggcctgtc tctggaagag accgccgaat ttgacaagga agagaccatc
     1861 gccgggatcg aggatgccct acgtgcgctc ggtcatacca cggagcggat cggtcatgcc
     1921 cgggcattga tccggcggct ggaggccgga gagcgctgga atctggtctt caacatctgc
     1981 gaagggctgc gcggcgtcgc gcgcgaggct caggtaccgg cgatcctcga actctacgag
     2041 gtgccgtatg tcttttccga cccgcttgtc cttgcgctga ccctgcacaa gggcatgacc
     2101 aagcgcgtgg tccgggatgc cggcataccg accagcgatt tcctcgtcta cgagacggat
     2161 gaggatctcg cggaactgcg gttcggtgcg ccgttctttg tgaaacccgt ggccgagggc
     2221 accggaaaag gctgttccgc caagtcggtt gtgcgggagc gtgccgcatt ggcggcggtc
     2281 tgtgacgatc tggtggcgcg cttccgtcag cccgcattga tcgaaccgta tcttcccggc
     2341 cgcgaattca cagtcggcat cacgggcacg ggccgtcaag cggcggtggc gggctccatg
     2401 gagatcgtgc tgttgccgca tgccgaagcg ggaggctact cgttcgacaa caaaggaaac
     2461 tatgaggatc gcgtggtcta tcccgccacc gatcccgcgc gggatccggt cgcggcaatg
     2521 gcggaacgta tcgcgctcga cgcctggcgc gcgttgggct gccgcgacgg cggccgggtg
     2581 gatcttcgct gcgacgcatc gggccgcccc cagtttctgg aggtcaaccc gctggcagga
     2641 ctgaacccca aggattcgga cctgccgatc ctcgcgcgtc agtgcggcat cacctatacc
     2701 gagctgatcg gacgtattct ggattcggcg attcagcggc aagcgggcgg ataacaggct
     2761 caaagcggac gggacggcac cccgttcctc ccgtgggaat ggggcgtctt tgcagcttgg
     2821 gaaggccccc cccgaaccgg attccgaatg gtcaaaaaca gaatcgcgcg tgaaaagggt
     2881 ggtactagac aggtcgcacc cgattctgta tacttaatcg ccttcaaccc aaaaaaagga
     2941 tgaatcatat gcgtatcaaa gcaaaagaac ccgtaaaaaa agtggtgaaa gccgtagcga
     3001 aagggaagga caagccggtg caaaccaaga cccggcgcac cgcgccgaaa acccagcgca
     3061 ttctgtttag cgtgcgcgcc gaagtcggca gccaggtttc ccttgcgggc agtttcaaca
     3121 attgggaccc gaccgccaaa cccatgacgg acaagaaggg tgacggggtt tacaccgtaa
     3181 ccgtgtcgct cgcgccggga acatacgaat acaagttcgt gattgacggc acctggtgtg
     3241 cggatccgga ctgcgccgat tgggtgcaga atgaacacgg cacgctcaac agtgtgaaat
     3301 acgtcaggta ggcggatacc ccgctttgcg cgaggcacac ggcaggagac gacgtatggc
     3361 ggacgccaga atacgttgcg ccctgacttt gggtgacggc gccggcgtgg gaccagaact
     3421 tgctctgcgg gcgttggctg atgaggaact ctcccgaaca tgccaactgc tcgtctacgg
     3481 gaaccggact cttctggagc gggtggccgt cgccagtggc atccccttcg ccgcgacgat
     3541 caaaatcctt gatggcgcgg aagggccttt gccacaggac ggccacgtgc tttttgattt
     3601 tccctttgcc gaagccgact cgatcacgcc cggaacggtt caggctgctt gcggtcgtca
     3661 ggcttatgaa tgggtgcgcc gcgcaaccct cgacatctcc cgtaaccagg tcgcgcacgc
     3721 tctggtcaca gccccgctca acaaatccgc cctgcacgcc gccggagtac aggcggtcgg
     3781 gcacacggag atcctgagcg aactgaccga gaccccggat ccgcgcatgt tgttttatgc
     3841 gccggctttt tgtgtctgtc tggcgaccat ccatgagccg ctggcgcgtg tttccgagtt
     3901 gctcaccgtg tcatccctgc tccgtacgct gcgtctgacg cacgaggcct gccggatgtt
     3961 cggcaccccc gcaccgcgac tcggcgtgtt ggcgctcaat ccgcatgccg gcgaaaacgg
     4021 cctgctcgga accgaggagc gtcgcgtcct catgccggct attgaaacag cgcgggccga
     4081 aggcattcac gcctccggcc cgctcgttgc cgacaccgct tttacatggc tcttgtcaac
     4141 cccgccgcaa tccgccccgt atgacgccta cgttgccatg taccatgacc aagggctgat
     4201 cccttttaag atcgccgcct tcgatcacgg tgtcaacgta acgctggggc tcccgatcct
     4261 ccgcacttcg cccgaccacg gaacagcgtt tgaccttgcc tggcagggaa ccgccagccc
     4321 ctccagcctg ttcagcgcca tccgacttgc cgtccgcatg gcgcggaacc gcatgccgcc
     4381 ttgcccttcc gtttcctgat cccgcgttca ttcgccgatg atcttcacca atacccgctt
     4441 ggaacgcaac ccgtcaaatt ccccatagaa aatctgttcc cacggaccga aatccaattt
     4501 cccccgggtt accgccacca ccacttcccg gcccataacc tgacgcttga ggtgcgcatc
     4561 cgcattatcc tcagccccgt tgtgcgcata ctgactgtga ggcttctccg gcgcgatccg
     4621 ttcgagccaa cgctcaaaat cggcatgcag ccccgcctcg tcatcattca caaaaacact
     4681 ggcggtaatg tgcatggcat tgaccagaca gaacccttcc tggattccgc tctcggccag
     4741 acaggcctct acttccggcg taatgttccg gtattcacgg cgatggtcgg tcttgaacca
     4801 taactcttta cggtaactct tcacgtttcc ccctttccga tttcccgcac gaccaacccc
     4861 ttcgggttgg ccagcacccg ttccccacat gcccaaaccg aaaccaccag ccccatcgcc
     4921 tccagttccg cagtcaccca ccgtaacttc gcttcaggca acagacaggt gatgatcacc
     4981 cctttgatcg aattgtcggc cgcataggac cggaccgttt caaaatcccc cagcacggga
     5041 taccccgcga tgatccgccc tttcaaattc acctcgtcgt caaggatccc gatgatgacc
     5101 cggtcatggt tgcccggtcg ctccatcagc tcgcgcagat acgcgcggaa ccgcaatccc
     5161 cccccgtacg cgaggaagcg caaggcagac gggttttcca gcagaacccg gcgctccagc
     5221 atctgcatga ccccgcgcac gcattcgcgc caagcccgga cacccgcaat cggaaaaatc
     5281 gccagcgtac cgaaccggac ggcgaaccgg atcatcccgc acccgtaatc attaagcaac
     5341 cagatcaatc ccgccccaat cgcagtcccc gccaaaaccg acaagcccaa gaccacgaaa
     5401 tcgcggatct gtgcgcgtcc ccatacgcgc gcatacgttt tggtcgcgac cagcgccata
     5461 aaaaccggca cgatgaaaac cggcaactcc gacaacacgg cggcccggtt aagggacagc
     5521 cccgactccc accgcaccac catccacgtg ccgcacaggg caaacacatc ccaaacgata
     5581 tagagcggga taagcaaccc ctgccgcatc atcccgtgcc gacctgaaag caaccgcccc
     5641 gtatcccaca actccacccg ttccaaatgc cggaccacaa tataaaccgc caccacaaac
     5701 gcaatcagaa acaaccccgg cgcgcgtcct ttcaacatgg ttccgaccaa accgatcaac
     5761 accaacactc cgctgatcac atacagaatg atcgcggctg tcttctgatt catggtctgc
     5821 cgcaacagcc ggtgatgcac atgatcctga tccggctgca tgaccctcac ccgtttgccg
     5881 acctccacca acccgctcgg cagacacgcc ctcaccgaac gccgccagat ggccagcagc
     5941 gtgtcgaaaa gcggcacccc catgacgagc aacggcatgc ccagcgaggc cgccaactct
     6001 ttacgcgatc cggtcatcag cggaaacgtc gcgatgcaca acccaagaaa catactcccg
     6061 gtatccccga ggaacaccga agccggatga aaattgtagc gcaggaaccc gagacaggcg
     6121 ccggccagca ccagatacgg caacgtggcc gcactcttcc ccgtgaacag caacgcgccg
     6181 atcagcccca tcgaagcgat caacgccaga cccgtcgcca acccgtccat cccgtcaatc
     6241 agattgaagg cgttgatagc ccccacaatc cacagtgtcg tcaccagaaa atcgagccac
     6301 ggcggaaacg ccacaataat cccgccgaca tggacgccgg agaggtacag gatcaccgcc
     6361 acaatgatct gccccgctaa tttgactacg ggcctgagcc ccctgaagtc atccagaaac
     6421 ccgatcaccg ccagcagagc cgaagcgccc agaaaatgcc cctgccaata cagggtgaat
     6481 tcggaggaga tcggcgaacg taacccccac accatcaccc cgagcaccag atgaaacgac
     6541 acgatgatcg ccagcccgcc tccgcgcggc accggttgct tgttaatgcg ccgcgcatcc
     6601 ggcagatcca tcatgccgat tttacgggcc gcctcacggc aaagcggcgt caacgccagc
     6661 gacaccgcca gcgccccaaa aaacgcgccc gcgtatttga ccccgtccgt cagcatccgg
     6721 accacaaaac cttccggaga atgcatccat tctgtgaccc aattgaccgt catacgcctc
     6781 tctcaacgcc cgaccattag tgcgggatac agttcagaca gaaacgcctc gaaagcctca
     6841 acctgttccg gggtcgccaa gccggatgcg acattcaccg cgaccatcac ccaccggttg
     6901 atccgattat gaatggaacg gtcccacaca tcgagccaga tccgctccat gtgcgaacag
     6961 ctttcccgct cccggctcat aaaccagtac aggagttgca tctcgcctcc acccggccgc
     7021 gcggctcgga tactgcggac ccgcaacggc gcacgccccg ccacccgtaa atccaaccgt
     7081 tcggcatcca acatcacata tccctgcgcc ggcagacaca attcggcccg gtgaatgctg
     7141 ctccggctac gtcccccaag aaccacactg accgcatacg aaaccccgtc caccgaacgg
     7201 taggtccgct tgagaatcac cgtgtcacgc ggcagttcgc tcgcctcccc cagcgagatc
     7261 gggaacaacg cgcccccgca accggggcac cgctgagccg gttccgcttc cgtcgccccc
     7321 gcaggctctt cgcccccttg cagacactgg gggttgtgac agaagagcgg tttccatccc
     7381 gtgaacaccc ccacacgttc cggcaacgtt ttcgcgataa acgaggcatc atcatacacc
     7441 gctggcggca tcgcgcgatt cgccgcaaac accgccaaca cagcagcgca caccccgcac
     7501 acaatccaca tccctccccc acttctctgt tctgcgttct gcgttccgca ttccgcgttc
     7561 tgcgttccgc attct
//
LOCUS       DAOSRB010000138         7467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9255_length_7467_cov_6.505262, whole genome shotgun sequence.
ACCESSION   DAOSRB010000138 DAOSRB010000000
VERSION     DAOSRB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9255_length_7467_cov_6.505262"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLZ74_07965"
     CDS             <1..293
                     /locus_tag="PLZ74_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98296.1"
                     /translation="AMIAVAIPNIGTWVKNPVLYEIGQTTVSPGTYNVIQGMSAIEKG
                     RYLVNAAGGSYVRAAFGTAWRQTGTTIGTGLTPGGYLLGLGIFETVDYQTRK"
     gene            304..486
                     /locus_tag="PLZ74_07970"
     CDS             304..486
                     /locus_tag="PLZ74_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98297.1"
                     /translation="MNIFELLTVIGVIVVGYFCGKYFGLHYGLGGWIGGFVLGVLLAI
                     AAYCIFRRLIGVTGKK"
     gene            763..972
                     /locus_tag="PLZ74_07975"
     CDS             763..972
                     /locus_tag="PLZ74_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98298.1"
                     /translation="MKQPLNIGISTEELNVADSILKATVNAFHTGSAAPRPAERDASG
                     RWIAPNVPMHPMSNGLVRIQFRVGQ"
     gene            1128..5444
                     /locus_tag="PLZ74_07980"
     CDS             1128..5444
                     /locus_tag="PLZ74_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98299.1"
                     /translation="MLIAVMLAAGRLCAQKMVYITQEQDCGIAPIPAELNEWRTGAMT
                     LRDPKPTPADVVVTGDDFFTREYGAPFSVEFNTGTNGWTRIFGTRGCQPKEVSIRFGR
                     GWGRQEGRKFQLQMDIEQSVAETEWDWAMWENGTRHKVPFKLTTSAGKRSRVVQDLGV
                     VRMPQRFSGVELTCRTKNVHVKILSAKIVPVTVPMTWRGIFVLGFDPWRAGMTLARRN
                     NYTLSINGTELSRGLSIDGGETLKMEIGEHLKKGTNIIEVVTEYHSGYADPGALLTEC
                     FAVSATGETTFLLAGPDWRVRVGDGPWLNPHYSRNPKSRAGFETLPSGKQVATGNQPM
                     HGGPLQVCHLGPKFPVFDCEDKTVEWQVSLPPRMTEATVACVVSNAITGEVAERLSRS
                     VAPQAGQHSTTAIFRLASRETGAYKLYWTLARGADVLDRVRTEMIVAGPVEQTAVPFN
                     EFDNLLESRMKVIQTRDCTVDELNPTNFAQYRGSAVVGEFPAEGLSRVGREGGLVFRE
                     TGERGGDWFGYKLDVEHLGRAHILEVDYPDTREQLLYVRVCESYPIHFCNNTARGGSG
                     HANASGAVRTGWMEPLTGKKEKLRILFYPGSKNITVTFENGGLGQTRAAVCGFRLYEV
                     PEGVPALELPPTDRLYGNHCERPLCGMWGASVASRFHYSWTEAFENVYAAAFAAAANR
                     IRFLRYSGQNLAIEGVYMYKQGFPTLSGESYLGVESFDFNYVLSKMYRRNGIRAFACF
                     EYIASPAIMLGGGYDIGDSEMRAAKGPLAPSYSVSRYGKQVVGFGGMGLNYLAPHVWR
                     SITNLVTEIYGRYEPTGGFEGLFMINNGWWIPGFPAASGDRLSEREVGYDDLSVTLFE
                     KDTGISLGIPYTPERFEKRYEALNSKHAQAWYAWRARKMRESLETISDLVAKGENKWA
                     VYASPGIRRTREDSPFDSIFSTAEERDTDYPRLLAEAGFPSDLYGRGTDARVTLVPRV
                     AFARAHDLKHWGAAMNAGARAIYAKQDAVYFDPTGLNERWTSTSGLDAWWWRAAGSTV
                     FDVKPSGTGAFFDMVDVCVDHTPTLMIHTWLDVNYPTAHGREGRRFATGFLASPVGDY
                     TDCPSVCGVQARRCGDAVQLVNDTPYALEGVLRSKTLGLFPHTVEDAFAGKKRGTFPY
                     ALEPYGITVLKGCNGSFSGEFRFADAAVAEQAMARTDALLANKLMCAKIKPQLIARLQ
                     KARLEKDAYTAVATLRDFEVRRVADAFFDHVITAKNTKPASELKVIGRYDGAEPKRFK
                     AGEPGQGLGAVDAKYKSATRARTLSLLVKTGGSDCAILLHDTPLRYDLRVSNGGLNAA
                     GLVFRVKPGQKGYFSYGLKDSDSVAANEWHHLCWTVDPEGSATLWVDGDLVDAVELDA
                     LHGVALDPASGGLNASRPTVPQGKTFADILIAREIFREGVMDWRDIQKEAAEWLGKGE
                     TK"
     gene            5441..7309
                     /locus_tag="PLZ74_07985"
     CDS             5441..7309
                     /locus_tag="PLZ74_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOR98300.1"
                     /translation="MRRLLIFSVAAFAAATQGAVIEEIRVPSPSMGKDIPACVVLPDK
                     YAADPGMAFSTVYVVHGAGGNYRTYATEELKFLADRHEFLFVCADGGKTSWWFDSPID
                     PSYRYETHVTKELVPWIDAHYRVIRDRAHRAIMGGSMGGHGACWLGFRHKELFGAIGS
                     IYGGMNLWNFPNSWDIAKRLGPRDEFPERWREHSVVTEAMKLKNGDVNLIMAVGTSDF
                     FLSANRELHNILTSNKVAHTYIEIRTAEEKTSGHSGEFNAMVKPIITAFIDNYFKNGE
                     AQIATAVTPFVTRYASKVTKAAHGTTPAPEIKVPAPWRVVEGDEAKTGLNVREYYFTI
                     EAPGRGLAAPRVELVWPGVAVKTVYGAKNMVRDGDRVLFTPTATKPPTGFTTGWSEGA
                     IRICVFHNVEGVQHGLYRDRPYPAAEVRAQNNWLFAARELFRRAGLAETNATEGAFVQ
                     LFGFESNFPNGHVDAPPHFHVMLMWNAWKENNVGHYRLDGKGRITENAFLVCGNVPGY
                     PRTGYFPQQLGETTHYNAPSGKTLFTLEMLRDGTGLVFRRPGVPGEYLLGSPEPVSSV
                     CVSFREDGTRPWRELARVSADDDSREGVLTIRTTVDGQCSVETVRYNPDTGERIKP"
BASE COUNT         1519 a         2154 c         2347 g         1447 t
ORIGIN      
        1 acgcaatgat agcagtggca attccgaaca taggaacgtg ggtcaagaat cctgtgcttt
       61 acgaaatcgg acagactacg gtttcgcccg gtacctacaa tgtcatccaa ggcatgagtg
      121 ccattgagaa agggcgctac ttggtcaatg ccgcaggtgg tagctacgtc cgggcagctt
      181 tcggtacagc atggcgccaa actggcacaa ccatcggaac tggacttacg cctggtggct
      241 atcttctggg acttggtatc tttgagaccg tagactacca gacacgaaaa tgaggtatcg
      301 tctatgaaca tctttgaact tcttacagtg attggtgtaa tcgtggtcgg gtatttttgc
      361 ggtaagtact tcggcttgca ttacggactt ggtggctgga taggcgggtt cgttctcgga
      421 gtcctgcttg ccatcgcagc atactgcatc ttccgccgat tgatcggtgt gaccggcaaa
      481 aagtaattct aggaaagaaa agggggattc gttcgcggag catcggtgtt acttcaatga
      541 accgtaatta caaagttatc atcgctaggt tgattagcgc actgttgtgt tgtttcggtg
      601 gcggggtagc acaatatttg ttatggcatc aatctggcga accgtggtac gaggctctgt
      661 ttcgttggtc atttctttgg tttttttatg ttcttttggc atcgttagcc ggagagcaca
      721 ttccaaaaat gctgtcaaaa aacgataatg aacgccgcgc acatgaaaca accactgaac
      781 ataggcatta gtaccgaaga gctgaacgtg gctgacagca tcttgaaggc cacggtcaac
      841 gctttccaca ccggcagcgc ggccccgcgc cccgccgagc gggacgcgtc gggacgctgg
      901 atcgcgccca acgtcccgat gcaccccatg agcaacggcc tcgtccgcat ccagttccgc
      961 gtcggccaat gaaccgtgga tatgccccgc atagttgggc acggcttttt ctttttgaaa
     1021 accgaaaccg gtgttgttag tattccttcc gattttgggt cacgaagaat atccggacag
     1081 aaaacatgag ggggaggccg gtgaagaagt tgttcaggca aagggtcgtg ctgattgcag
     1141 tcatgctggc ggcggggcga ctgtgtgcgc agaagatggt ttacatcacg caggagcagg
     1201 actgcggcat tgcgccgatc ccggctgaac tgaatgagtg gaggacgggg gcgatgacgc
     1261 tccgcgaccc gaaaccgacc cctgcggacg tggtcgtcac gggtgatgac ttctttaccc
     1321 gcgaatacgg tgcgccattc agcgttgaat tcaacacggg aacgaacggc tggacgcgga
     1381 tcttcggcac aaggggatgc cagcccaagg aggtttcgat ccgtttcgga cgcggctggg
     1441 gccgtcagga gggacgcaag ttccaactgc agatggacat cgagcagtcc gttgccgaaa
     1501 cggaatggga ctgggccatg tgggaaaacg ggacccgcca caaggtaccc ttcaagctca
     1561 caacctccgc cggaaagcgc tcacgggtgg tacaggatct tggcgtggtg cggatgcccc
     1621 agcgtttcag tggcgtggaa ctcacgtgcc gcacgaaaaa tgtccacgtc aagatcctct
     1681 ccgccaagat cgtgccggtg accgtgccga tgacctggcg cgggattttt gtgctcggct
     1741 tcgatccgtg gcgcgccggc atgacccttg cgcggcgcaa taactacacg ctctctatta
     1801 acggaacgga gctttcgcgg gggctttcca tcgacggcgg cgaaacgctc aaaatggaaa
     1861 tcggtgaaca cttgaagaag ggcacaaaca tcattgaggt cgtcacggaa tatcacagcg
     1921 gctatgccga tcccggcgcg ctgctgacgg agtgtttcgc cgtgtcagcg acaggcgaga
     1981 caacgtttct gttggccggg cccgactggc gggtgcgcgt cggcgacggc ccgtggctga
     2041 accctcatta ttcgagaaac ccgaagagcc gagcggggtt cgagaccctt cccagcggca
     2101 agcaggtcgc gacaggcaac caaccgatgc acggcggccc gttgcaagtg tgccatctgg
     2161 ggccgaagtt ccccgttttc gactgtgagg acaagacggt cgagtggcag gtttcgcttc
     2221 cgccccggat gacggaggcg accgtcgcct gcgtggtgtc aaacgccatc acaggcgaag
     2281 tggcggaacg gctctcccgg tctgtcgcgc cgcaagccgg acaacattcc acgaccgcca
     2341 tttttcggct tgcctcccgc gaaaccggcg cctacaagct ctactggacg ctcgcgcgcg
     2401 gtgccgacgt gctcgaccgt gtgcgcacag agatgatcgt ggcgggacct gtggagcaaa
     2461 cggcggtacc gttcaacgaa ttcgacaacc tccttgaaag ccgcatgaag gtgatccaga
     2521 cgcgcgactg cacggtggat gagcttaacc cgacaaattt tgctcagtat cgcggcagcg
     2581 cggttgtcgg cgaattcccc gccgaaggat tgtcgcgtgt cgggcgcgaa ggcgggctcg
     2641 tgtttcgcga aacgggtgag cgcggcgggg attggttcgg ctacaaactc gacgtggagc
     2701 atctcggacg ggcacacatt ctggaggtgg actaccccga cacgcgtgag cagcttctct
     2761 acgtgagggt gtgcgagtca tatccgatcc atttctgtaa caacacggcg agaggcggat
     2821 cgggacatgc gaatgcctcc ggagcggttc gcaccggatg gatggagccg ctcaccggca
     2881 aaaaagagaa gttgcgtatc ctcttttacc ctggctccaa gaacatcacc gtcacgttcg
     2941 agaacggcgg tctgggccag acacgggcgg cggtctgcgg attccgtctg tacgaggtgc
     3001 cggagggtgt tcccgcgctc gaactaccgc caacggatcg cctctacggc aaccactgcg
     3061 agcggccgct ctgcggcatg tggggcgcgt ccgtggccag ccgcttccac tacagttgga
     3121 cggaggcgtt cgaaaatgtt tatgctgccg cgtttgccgc cgccgcgaac cgcatccgct
     3181 tcctgcgtta ttcgggacag aacctcgcga tcgaaggcgt gtacatgtat aagcagggtt
     3241 tccccacgct ctcgggggag agctatctgg gcgtcgagtc cttcgacttc aactacgtgc
     3301 tctcgaagat gtaccggcgc aacggcatcc gcgccttcgc ctgtttcgag tacatcgcct
     3361 cgccagccat catgctgggc ggcggctatg acatcgggga cagcgagatg cgtgcggcga
     3421 aaggccccct cgcgccttcc tattcggtaa gccgctacgg taagcaggtg gttggctttg
     3481 gcggcatggg gctcaattac ctcgcaccgc acgtgtggcg gtcgatcacg aatctggtta
     3541 cggagattta tggccgttac gaaccgacgg gcggcttcga aggcctcttc atgatcaaca
     3601 acggctggtg gatccccggc tttccggccg caagcggcga cagactctcc gaacgcgagg
     3661 tgggttacga cgacctttcc gtaacgcttt tcgagaaaga tacggggatt tctctcggga
     3721 tcccctatac gcctgaacgc tttgagaaac gttatgaagc cctcaattcg aaacatgcgc
     3781 aggcgtggta cgcttggcgc gcccgaaaga tgcgcgaatc gctggaaacg atcagcgacc
     3841 ttgtcgcgaa aggcgagaac aagtgggccg tctatgcctc gcccggcatc cggcgcacgc
     3901 gggaggatag cccgttcgac tcgatctttt ccaccgcaga ggaacgggac actgactatc
     3961 cccgccttct tgcggaggcc ggtttcccgt ccgacctcta cgggcggggt acagacgcgc
     4021 gggtcacgct cgttcctcgg gtcgcgttcg cgcgagccca cgacctgaaa cactgggggg
     4081 ctgcgatgaa cgcgggagcg cgcgcgatct acgcgaagca agacgccgtt tacttcgatc
     4141 ccaccggcct gaacgagcgt tggacctcca cgtccggcct cgacgcatgg tggtggcgcg
     4201 cggccggctc gaccgtcttt gacgtaaaac cctccggtac cggcgcgttc ttcgatatgg
     4261 tggacgtttg cgtcgaccac acgccgacgc tgatgatcca cacatggctt gacgtcaact
     4321 accccaccgc gcacggacgc gagggacggc ggttcgccac cgggttcctc gcgagccctg
     4381 tcggcgatta cacggactgc ccgtcggtct gcggcgtgca ggcgcgccgt tgcggcgacg
     4441 cagtgcaact cgtgaacgac acgccgtacg ctttggaggg cgtgctgcgt tcgaagacgc
     4501 tggggctttt cccgcacacg gtcgaggacg ctttcgccgg caagaagcgc gggacgttcc
     4561 cgtacgcgct cgaaccttac ggcatcacag tgctgaaggg atgcaacggc tcgttttcgg
     4621 gtgagttccg tttcgccgat gcggctgttg cggagcaggc catggcgcgg acggacgcgc
     4681 ttctcgcaaa caaactcatg tgcgcgaaaa tcaagccgca actcatcgcg cggctccaaa
     4741 aggcgcggct ggaaaaggac gcctacacgg cagtggccac gttacgcgat ttcgaggtgc
     4801 gccgagtcgc ggacgctttt ttcgatcatg tcatcaccgc gaaaaacacg aaacccgcat
     4861 ctgaactgaa agtgatcgga cgctacgacg gcgccgaacc gaaacgtttc aaggccggtg
     4921 agcccggtca gggattgggt gccgtggatg caaaatacaa gagcgccacg cgggcgcgaa
     4981 cgctatcgct gcttgtcaag accggcggct ccgactgcgc gattctgctg cacgacacac
     5041 cgctccgtta cgatctcagg gtaagcaacg gcggactgaa tgcggcgggc ctcgtctttc
     5101 gtgtcaaacc gggccagaag gggtatttct cttatgggct gaaggattcc gattcggtgg
     5161 ctgcgaacga gtggcatcac ctgtgctgga cggtcgaccc ggaggggtcg gcgacgttat
     5221 gggtggacgg cgatttggtg gatgcggtcg agcttgatgc acttcacggc gtggcactcg
     5281 accccgcgtc aggagggctc aacgcatcgc gaccgactgt cccgcaaggc aaaacgtttg
     5341 cggacatcct gatcgcgcgc gaaatctttc gtgaaggcgt gatggattgg cgcgacattc
     5401 agaaagaggc cgccgagtgg ctcggtaaag gagagacgaa atgaggagac ttttgatttt
     5461 ttccgtggcc gcgttcgcgg cggctacgca aggcgcggtg attgaggaga tccgggttcc
     5521 gagcccgtcg atgggcaagg acatcccggc ctgtgtggtc ttgccggaca agtacgccgc
     5581 cgatccgggc atggcgttca gtacggtcta cgtggtgcat ggcgcaggcg gcaactaccg
     5641 cacgtacgcg acggaagaac tgaagtttct ggccgaccgc cacgaattcc tgttcgtctg
     5701 cgccgacggc ggaaagacga gctggtggtt cgattcgcct atcgacccgt cgtatcgtta
     5761 cgaaacccat gtaacaaagg aactcgtgcc atggatcgat gcgcactacc gtgtcatccg
     5821 cgaccgggcg catcgcgcga tcatgggcgg atccatgggc ggccacggcg cctgctggct
     5881 cggattcaga cataaggaac tcttcggcgc gatcggcagt atctacggag gcatgaacct
     5941 gtggaatttt ccgaatagct gggacatcgc caaacgcctt ggcccgcgtg atgaattccc
     6001 cgaacgctgg cgggagcact ctgtggttac agaggccatg aaactgaaga acggcgatgt
     6061 gaacctgatc atggccgtgg gcacatcaga cttcttcctg tccgcgaacc gcgagttgca
     6121 caacatcctt acgtcgaaca aggtggcgca cacctacatc gagatccgca cggccgagga
     6181 gaagaccagc ggccactcgg gcgagttcaa cgccatggtg aagccgatca tcacggcatt
     6241 catcgataat tattttaaga atggtgaggc gcagatcgcc acggccgtga cgccgttcgt
     6301 cacgcgctat gcctcgaagg tgacgaaggc cgcgcacggc acgactcccg cacccgagat
     6361 caaggtgccc gcaccatgga gggtggtgga aggggatgag gcgaaaacgg ggctcaacgt
     6421 gcgcgagtat tatttcacga tcgaggcccc cggacgcggc ctcgccgcac cgcgcgtgga
     6481 actcgtctgg ccgggtgtcg cggtgaaaac cgtgtatggg gcgaagaata tggtgcgcga
     6541 cggcgaccgt gtccttttca cgcccacagc gacgaagccg cccaccggtt tcaccacggg
     6601 gtggagtgag ggtgcgatcc gcatctgcgt cttccacaat gtggaggggg ttcaacacgg
     6661 tctgtaccgc gacaggccct atcccgctgc ggaggtccgt gcgcaaaaca actggctgtt
     6721 cgccgcgcgc gaattgttcc ggcgcgccgg actcgccgag acgaacgcga ccgaaggcgc
     6781 gtttgtgcag cttttcgggt ttgagtccaa cttcccgaac ggccatgtgg acgcgccgcc
     6841 gcacttccat gtgatgctga tgtggaacgc ctggaaggaa aacaacgtgg ggcattaccg
     6901 tctcgacggg aaaggacgca tcacggagaa cgccttcctc gtctgcggga acgtgcccgg
     6961 atatccccgg accggctact tcccgcagca actgggcgag acgacgcact acaacgcccc
     7021 ctccggaaag acgctcttca cgcttgaaat gctccgggac ggcaccggtc tcgtgttccg
     7081 ccgtcccggc gtgcccggcg agtacctgct cggttctccc gagcctgtct catcggtctg
     7141 cgtttcgttc cgcgaggacg gcacgcgccc ctggcgtgaa ctcgcgcgcg tgtcggcaga
     7201 cgatgactcc cgcgagggtg ttctgaccat ccggacaacg gtggacggcc aatgctcagt
     7261 cgaaaccgtc cgctataatc ccgacaccgg cgaacggata aaaccataag aggctcctgc
     7321 cccgcgaccg gcgacattag ccaaacgcaa aggttgccaa agtaatcagt ttgtgacagg
     7381 ggtgtgcgaa gacaatgcgt ttctggtgaa aaggggggca gtgagtcggg agcgcgggcg
     7441 tccccgcccg caacagcgtg tcttgcg
//
LOCUS       DAOSRB010000139         7369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9393_length_7369_cov_6.783019, whole genome shotgun sequence.
ACCESSION   DAOSRB010000139 DAOSRB010000000
VERSION     DAOSRB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9393_length_7369_cov_6.783019"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..323
                     /locus_tag="PLZ74_07990"
     CDS             93..323
                     /locus_tag="PLZ74_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98301.1"
                     /translation="MNTLCSKNRSFLYRKALPMLVPFLSVFTGPPQPGLLPPTAIRAS
                     FRADRETKQQMLAAAGDETNYKRDKQYRRWSI"
     gene            386..781
                     /locus_tag="PLZ74_07995"
     CDS             386..781
                     /locus_tag="PLZ74_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98302.1"
                     /translation="MFTPFPSFCRDAAGGFIRLFVLTLLCGCAALRFDVTRKARFINM
                     DSEILHVDYGSEKRSETLPNGLVCTFEGKVRLLLPGDNRRVTLYQAISTTGVRYVSED
                     KQLEFIEKGPYCIVLRNGQTIFEGVYCRP"
     gene            complement(766..1791)
                     /locus_tag="PLZ74_08000"
     CDS             complement(766..1791)
                     /locus_tag="PLZ74_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOR98303.1"
                     /translation="MGKTLSVQGIVELASAYYGSAVLFAAVESDVFTRIAAAGGEAEI
                     GPLAEDLGADPRGLRLLLDACVAIGLLTKTEERYAVTPAAAMTLVAGAPQDLTRAVRY
                     NRDVYPAWGRLTELVRTGRPVEAPECHLGADADRTRRFVLSMHGRALGIGRGVVPLID
                     VSGCRTLLDLAGGPGTYAVLLAQANPGLRCVTVDLPEISAVAAELVDGAGMADRVICR
                     PGDYHTDRYEPEAYDAVTIFGALHQESPERIPDILRRAADALRPGGRIHILDMMTDAT
                     HTWPAFSALFAVNMALTTRDGWVFSDGELHGWLREAGFEACATLPVPPPMPHWLVSAR
                     KPDHGRQ"
     gene            complement(1827..3158)
                     /locus_tag="PLZ74_08005"
     CDS             complement(1827..3158)
                     /locus_tag="PLZ74_08005"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOR98304.1"
                     /translation="MRWIVYNLLFAFVYTAMMPVFLFRMKRRGGYRERMGDRFGCYPD
                     GIARQLRDMRGAVWIHAVSVGEVYVAGQVMRALRERIPSLRFVFSTTSSTGWREAEKQ
                     LGTSDVLIYNPLDFPVCVRRAMSRIRPAALILTESEIWPNLIRACHGCGVPLYLVNAR
                     VSDRSAPGYRRLRFWFGPVLRKFTAILAQSEEDRRRLVAAGADPARVEVTGSFKFDVA
                     NRDPEAEKAVAAYLDRWDAGAGRVVLLGGSIWPGEDVVLITLYARLRRIFPALRLVLV
                     PRHFEKADTVEAAIRRAGFACARKSRTPLPEEGTGAPEGGATIVLGDTTGELMGFYGN
                     AALVFVGKSLCEHGAQNMIEPCLCGAATLIGPHTENFRPVVSDLLEAGALIQVPDAEA
                     LEREIARLLADDAARRALGERASAAVLRRKGVVGRCADTLAAELRKTGCVR"
     gene            complement(3295..5718)
                     /locus_tag="PLZ74_08010"
     CDS             complement(3295..5718)
                     /locus_tag="PLZ74_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98305.1"
                     /translation="MTRRTQRTGWRALAAAGLLGAAGAVSAASLDLNRDGVVRVAVLS
                     APRQAGGMGMDAFVADLESMIRAADASVPARVTLEPVGDGCRTLMGWWYHPDRQSERK
                     RLLTGQYDLLLLAEDESVVLGYPEFFFEGVRALSQAAGGARIRPALVLLAKPATSFRD
                     KRLDAVAELTYRVGDGCGVEVIPAAYAWYETLRRNRMSGNSSVRVRAATFLAAATVYT
                     HLSGDRVPKAAWETGWTTKKITSALVQSATEAVANERVRAHYKGPFRGVVRIDPRITR
                     RLKVYVPNTAEDDPVRQNLQYILDAAFQDGFWRSPSDWYRSGFDRYAAAFDLVYGDRQ
                     QMDLYLDPARYTSLGMGVATNGPQPCAAVYSRTPQGDGVDGRDTLRTLEANLLEGYDH
                     AKANGLTFIPYPLAWARVYRENAELVREVVPGSANDWLVYMLANMLYTVVTDRCQPPP
                     DKRKPGGGNGAHPHGYHEVCARIGYETVVQLANLSQPVNTLLLRSETYRIDRQNPGFA
                     GVRLLNRPSSEVRVFCATDIPGVAQLSRETLVFTPENYDIEQTVRVAPATDRPTQFFH
                     FMASAQSEDRAIDGANDLRPFLLNYDASEEGSVVFDRDHLSPQTGFDAVLRPEQRPCD
                     LVRVRVEQHGQVTAEIFFSPDYFSGMPVRIMPSAADYQNGFAQVTVRTLSADERFNNR
                     TFLHTFRVSSGSDPVPQIRVTAPAADSVLDGPAFVAARAEAERSDRVRSVAIYLGPKL
                     LGNSSTPVCAVSVEQAPPQSRLGGGDYTLRAVAIRDDGLVVSSPLLSFRVREPVGQGA
                     PLSNGPPSP"
     gene            complement(5715..6839)
                     /locus_tag="PLZ74_08015"
     CDS             complement(5715..6839)
                     /locus_tag="PLZ74_08015"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98306.1"
                     /translation="MNGFLCLICGDDDYLVEAAARERIARFLPEADRALGLEIIDGRR
                     DTGDEARKAAEACMESVQTPGFFGAVKVTWLRNATFLTGGGRVAESLAAKEAVERLTI
                     WLKGGLEEGQVLVITAARILRSSKFFKLCQKQGEVEDFGGNLKSWELEKRASQRLDGL
                     LKKAGLTLDEEAYREFLNRVGWETRLLVQEVEKLRLYIGASGRVTIEDVRTITSVGRE
                     AEAWDLLDAFGERKPAEMLKALSRMDGQKGIGIMLAVMLEKRVRELLVLRAAYDRKWL
                     TTSGWQRDLPPEAVEQLAALPVNDKTMSAWMLKKRMPQVLAYSLQELRGARFRLLDLR
                     EKLVSTGLPERFLIETVLLRIMARRAVSRPAARQRVAGAR"
     gene            complement(6897..>7369)
                     /locus_tag="PLZ74_08020"
     CDS             complement(6897..>7369)
                     /locus_tag="PLZ74_08020"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR98307.1"
                     /translation="NVRAPFSTNLLLGVMSAGCAWATFGKFPFWMVPMAPDHEEMLPL
                     KGGRMPEPESMETSPVSPDRLTDVFMSGTIHDEDQPCHLKILDRAKCEECIKLYGAPC
                     QRFCPAEVYRLEDGHIHVDFSNCLHCKTCQIKDPFQNIVWDFPQGGDGPRYTRM"
BASE COUNT         1434 a         2559 c         2135 g         1241 t
ORIGIN      
        1 atcgccgcga ggatgcggat atcgccaaag agtatcatga ctccatgcat aagtaatgcg
       61 cgaaggaaga ggaaaggaag gcagcgttct tgatgaacac tctttgctca aaaaaccggt
      121 cgttcctcta tcggaaggcg cttccgatgt tggttccgtt tctctccgtt ttcacgggac
      181 cgccgcaacc cgggctcctc ccgcctaccg ccatacgcgc atcgttccgc gccgacaggg
      241 agacgaaaca gcagatgctg gcagcggcag gggacgaaac gaattacaaa cgcgacaaac
      301 aataccgccg ctggagcata taacgcaggc acgatcggca cggcgacgct tgcggcaccg
      361 acccaaggag gggtatactg tacggatgtt caccccattc ccatctttct gccgtgatgc
      421 ggcgggtggt ttcattcggt tgtttgtgct gacgctgctg tgcggctgtg ccgcgttgcg
      481 ttttgacgtg acgcgcaagg cgcgcttcat caacatggat tccgagatcc tgcatgttga
      541 ctacggttcg gaaaagcgca gcgaaaccct gcccaatgga ctggtctgca cttttgaagg
      601 caaggtgcgc ctcctcctcc ccggagacaa taggcgggtg acgctctatc aggcgatctc
      661 caccacgggt gtgcggtatg tctcagagga taagcagctg gagttcatcg aaaaaggccc
      721 ctattgcatc gtgctccgaa acggccagac gatcttcgaa ggggtctact gccgaccgtg
      781 atccggctta cgcgcactga ccagccagtg cggcatcggt ggcggcacgg ggagcgtcgc
      841 acacgcttcg aatcccgcct cccgcaacca accgtgcaac tccccgtccg agaagaccca
      901 tccgtcgcgc gtggtgagcg ccatgttcac cgcaaacagc gcggagaaag ccggccacgt
      961 gtgagttgcg tcggtcatca tgtccagaat atggattcgt ccgccggggc gcaaggcgtc
     1021 ggccgcgcgc cgcagaatat ccgggatccg ttccggtgat tcttgatgca gggcaccgaa
     1081 aatcgtcacc gcgtcgtagg cctccggctc atatcggtcc gtatggtaat cgccggggcg
     1141 gcagatgacg cgatccgcca tcccggcccc gtccaccaat tcagcggcca cagcggagat
     1201 ctccggcaga tcgaccgtca cacaccgcag cccgggattc gcctgtgcca gcagcaccgc
     1261 ataggtgccc ggaccgcccg ccagatccaa cagggtacgg catccgctca cgtcgatcag
     1321 cggcaccacg ccacgcccga tccccagtgc tcggccatgc atcgagagca cgaaacgtcg
     1381 ggtgcggtcg gcgtccgcgc cgagatgaca ctccggggcc tcaaccggcc tgccggtccg
     1441 gaccaactcc gtgagccgac cccatgccgg atacacgtcg cggttgtacc gtaccgcacg
     1501 cgtgagatcc tgcggtgcgc ccgccaccag cgtcatcgcg gcggccggtg tcacagcgta
     1561 ccgctcctcg gtcttcgtca gcaggccgat ggccacacag gcatccagca acaaacgcag
     1621 accgcgcggg tccgctccca gatcctccgc caacgggccg atctccgcct ccccgcccgc
     1681 tgcagcgatc cgcgtgaaca catcggactc aaccgccgcg aacaataccg ccgagccata
     1741 atacgcgctg gccaattcaa caatcccctg caccgacagc gtcttgccca tcccgtctct
     1801 cccgttcttt cctttgctcc gggcccctac cggacgcatc cggttttccg caactccgca
     1861 gccagtgtgt cggcacaccg cccgaccacc cccttgcggc gcagaaccgc cgcagacgcg
     1921 cgttccccga gcgcgcgccg cgccgcgtca tccgccagca accgggcgat ctcgcgttcc
     1981 aacgcctcgg cgtccggcac ttggatcagt gccccggcct ccagcagatc ggacaccacc
     2041 ggacggaaat tttccgtatg cggtccgatc agggtggcgg cgccgcagag acagggctcg
     2101 atcatgtttt gcgcgccgtg ttcacagagg cttttgccga caaacaccag cgccgcattc
     2161 ccatagaagc ccatcagttc gccggtggtg tcgcccagca caatcgtcgc gccaccctcc
     2221 ggtgcccccg tcccctcctc cggtaacggg gtccggctct tccgcgcaca agcgaacccg
     2281 gcccgccgga tcgcggcttc taccgtgtcc gccttttcaa agtgccgcgg caccagcacc
     2341 agccgcaatg ccggaaagat gcgccgcagc ctggcgtaga gtgtgatcag cacgacatcc
     2401 tcgcccggcc agatcgaccc gcccagcaac acgacacgcc cggcccccgc atcccaacga
     2461 tccagatatg cggcgacagc cttttccgct tcaggatcgc ggttcgccac atcgaatttg
     2521 aaactgccgg tgacttcgac gcgcgcaggg tcagcgcctg cggcgaccag ccgccgccgg
     2581 tcctcttccg actgggccag aatcgcggtg aacttgcgca acaccggccc gaaccagaaa
     2641 cgcaggcgtc ggtatcccgg tgccgaacga tccgatacgc gcgcgttgac cagataaagc
     2701 ggcacgccgc aaccgtggca ggcacggatc aggttgggcc aaatctccga ttccgtcagg
     2761 atcagggccg caggccgtat ccgactcatc gcccgccgga cgcacacggg aaaatccagc
     2821 gggttgtaga tcagcacatc cgacgtgccg agttgtttct ccgcctcacg ccatccggtg
     2881 gaactggtgg tgctgaacac gaagcgcagc gaagggatcc gctcccgcag cgcccgcatc
     2941 acttgcccgg ccacatacac ctcacccacg ctcacggcat ggatccacac cgccccgcgc
     3001 atgtcgcgga gttgccgcgc gatgccgtca ggataacatc cgaaccggtc gcccatacgc
     3061 tcgcggtacc cgccgcgccg cttcatccgg aagaggaaga ccggcatcat cgccgtatac
     3121 acgaaagcaa acaacaggtt gtaaactatc cacctcatgg accggtccca tccctccgcc
     3181 gtaccgtgcg cggcataccg cacccgaccc gttttgaaag gtataagtgt aggtgcgcag
     3241 tgttcagcct gtcaagacgc ggaacgcggt catccgaaat tccgacgcca aaggtcaggg
     3301 ggacggcggg ccgttcgaga ggggggcgcc ctgaccaacg ggttcgcgca cacgaaacga
     3361 gagcagcggg gaagaaacca ccagcccgtc gtcgcggatc gccacggcgc gcaacgtgta
     3421 gtcgcccccg ccaagacggg actggggcgg ggcctgctcc accgacaccg cgcagaccgg
     3481 tgtggacgaa ttccccagca gtttaggtcc gagatagatc gccacagacc tcaccctgtc
     3541 ggaccgttcc gcttcggcac gggccgccac aaaagccggt ccgtccagaa cactgtccgc
     3601 cgcaggcgcg gtgacgcgga tctgcggcac gggatcactg ccgcttgaaa cgcggaaagt
     3661 gtggaggaag gtgcggttat tgaaccgttc gtccgcggaa agggtgcgta ccgtgacctg
     3721 cgcgaacccg ttttggtaat ccgcggccga gggcatgatc cgaaccggca tgcccgagaa
     3781 ataatcggga ctgaaaaaga tctccgccgt aacctggccg tgctgctcca cacggacgcg
     3841 caccagatca caaggccgtt gctcaggccg caacaccgca tcgaatccgg tctggggcga
     3901 aaggtgatcg cgatcaaaca cgacactccc ctcctccgac gcatcgtaat tcagcagaaa
     3961 gggccgcaga tcgttcgcgc cgtcgatcgc ccggtcttcg gattgcgcac tggccatgaa
     4021 atgaaagaac tgcgtgggac ggtcggtcgc aggcgcgacc cgtacggtct gctcgatgtc
     4081 gtagttttcc ggggtgaaca ccaaagtttc acgcgacaac tgggcgacgc cgggaatgtc
     4141 ggtggcgcaa aagacacgga cctcgctcga agggcggttg agcaatcgga cccctgcgaa
     4201 accggggttc tggcggtcga tccggtaggt ctcgctgcgc aaaagcaggg tgttcacggg
     4261 ttgagacagg tttgccaact gcacgaccgt ctcgtacccg atacgggcgc acacctcgtg
     4321 atagccgtgt ggatgggctc cgttcccccc tcctggcttg cgcttgtcgg ggggcggctg
     4381 gcagcggtcg gtgaccacag tgtagagcat gttcgcgagc atgtagacga gccagtcatt
     4441 ggcgcttccc ggcacgacct cccgcacgag ttcggcattc tcacggtaca cgcgcgccca
     4501 tgcgagcgga tacgggataa aggtcaggcc gttggccttg gcgtgatcat acccctccaa
     4561 caggttcgcc tccaaggtcc ggagcgtgtc acgcccgtcc accccatcgc cctgcggcgt
     4621 gcgggagtag accgccgcgc agggctgggg cccgttggtg gcaacaccca tgcccaacga
     4681 ggtgtaccgc gcgggatcca gatacagatc catctgctgc cggtcgccgt aaacgagatc
     4741 gaacgccgcg gcataccggt cgaatccgga acggtaccag tcggaaggcg accgccagaa
     4801 cccgtcctga aacgcggcat ccaaaatgta ttgcagattc tggcggaccg gatcatcctc
     4861 ggccgtgttg ggcacataca ccttgagacg tcgggtgatg cgcggatcga tccgcaccac
     4921 cccgcggaac gggcctttgt agtgagcgcg gacccgttca ttggcaaccg cctctgtcgc
     4981 ggactgcacc agcgcggacg tgattttttt ggttgtccaa ccggtctccc atgcggcttt
     5041 gggcacacga tcgccactca ggtgcgtata gacggtggcg gcggcaagaa aggtcgcggc
     5101 ccgtacccgg accgaagagt tgccggacat gcggttgcgc cgcaacgtct cgtaccaggc
     5161 gtatgccgcc ggaatcacct ccacgccgca gccgtcgccc acacgatagg tcaactcggc
     5221 caccgcgtcc aggcgcttgt cccgaaacga ggtggccggt ttggcgagca gcaccaacgc
     5281 cggccggatc cgcgcacccc ctgccgcctg cgacaaagcg cgcaccccct cgaaaaaaaa
     5341 ttcggggtat cccagcacca ccgactcgtc ctctgccagc aacagcaaat cgtattgtcc
     5401 ggtcaacaga cgcttgcgct cgctttggcg gtcggggtgg taccaccatc ccatcaatgt
     5461 gcggcacccg tcaccgacgg gctcgagggt cacacgcgca ggcacactgg catccgccgc
     5521 ccggatcatg ctttcgaggt cggcgacgaa cgcatccata cccattccgc ctgcctgtcg
     5581 cggggccgac agcaccgcca cccgcaccac tccgtcccgg ttgagatcca gcgaggccgc
     5641 cgacacggca ccggcggccc ccagcagtcc cgctgcggcc agcgcgcgcc atcccgttct
     5701 ctgcgtcctt cgcgtcatcg cgctcccgcc acccgttgcc tcgccgcagg ccgagaaacg
     5761 gcgcggcgcg ccataatccg caacagcacg gtttcgatca ggaaacgttc aggcagaccc
     5821 gtggagacga gtttttcgcg cagatccaac agccggaacc gggcgccccg caattcctgt
     5881 agcgaatacg ccaacacctg cggcatccgc tttttcaaca tccacgcgct catcgtcttg
     5941 tcgttcaccg gcagggcggc gagttgttcg accgcctcgg gcggcaaatc ccgctgccat
     6001 cccgaggtgg tgagccactt gcggtcgtat gccgcacgca gaaccaacaa ttcgcggacc
     6061 cgcttctcca acatcacggc gagcatgatt ccgatcccct tctgcccgtc catccgcgac
     6121 agtgccttca acatctccgc cggcttgcgc tcaccgaacg catcgagcag atcccacgcc
     6181 tcagcttcgc ggccgaccga agtgatcgtc cgtacatcct caatcgtcac ccggccggac
     6241 gcaccgatat aaagacgcag tttctcgacc tcctgcacca gcagccgcgt ctcccagcct
     6301 acccggttca gaaattcgcg gtacgcttct tcgtccaagg tcaacccggc ctttttaagc
     6361 agcccgtcca gacgttgcga agcccgcttc tccaactccc aggacttgag gtttcctccg
     6421 aaatcttcca cctcgccctg cttctggcag agcttgaaaa atttcgagga ccgcaaaatc
     6481 cgggctgcgg tgatcaccag cacctgcccc tcttccaacc cgcccttcag ccagattgtc
     6541 agacgctcaa ccgcttcctt ggcagccaaa ctctccgcaa cccgtccgcc gccagtcaga
     6601 aatgtagcgt tgcgtagcca cgttaccttg accgcgccga agaaacccgg ggtctgcacc
     6661 gattccatgc acgcttccgc cgccttgcgg gcctcgtccc cggtgtcgcg gcgcccgtcg
     6721 ataatctcca gcccgagcgc acggtccgcc tccggcagaa accgcgcgat ccgctcccgg
     6781 gcggccgctt cgacgagata atcgtcatcc ccgcagatca gacacaaaaa accattcatg
     6841 tcgtgagtat agcggaaccc cggtgcaatg gcgagaagga ccgccccgta tcaactttac
     6901 atgcgtgtgt aacgggggcc gtcaccgccc tgcggaaagt cccacacgat gttctggaac
     6961 ggatccttga tctggcaggt cttacagtgc aggcagttgg agaagtccac atggatgtgg
     7021 ccgtcctcca agcggtacac ctcagccggg cagaagcgct ggcagggcgc gccgtagagc
     7081 ttgatgcact cctcgcactt cgcgcggtcc agaatcttca ggtggcacgg ctgatcctcg
     7141 tcgtggatcg tgccggacat gaagacgtcg gtcaggcggt cgggcgagac cggactggtc
     7201 tccatgctct ccggctccgg catgcgcccg cccttgagcg gcagcatctc ctcgtggtcg
     7261 ggtgccatcg gcaccatcca gaaggggaac ttgccgaagg tcgcccaggc gcaacccgcg
     7321 ctcatcacgc caagcaacag gttggtcgag aaaggcgcgc gcacgttct
//
LOCUS       DAOSRB010000140         7343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9437_length_7343_cov_5.737239, whole genome shotgun sequence.
ACCESSION   DAOSRB010000140 DAOSRB010000000
VERSION     DAOSRB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9437_length_7343_cov_5.737239"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2961)
                     /locus_tag="PLZ74_08025"
     CDS             complement(<1..2961)
                     /locus_tag="PLZ74_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98308.1"
                     /translation="MKSRLSFDSCFISAWTFAVAAFCFQMGAQAQTSVSLYKGPITGS
                     SPENPVDFSVTSYWDPAGMPSGTTAEAKFAKATMGTLTEQMYVALPNNLTLLTLRAGG
                     GKIINFIGENVSLGTLHYHDYLGYASVLYATAHAFGNDISSVCGAFAGDVVNAISGKM
                     VFAGGEVEHHHHCYANSDSETILNALPTNFWTHGSANLHLYASRQCGEVTGLWKATKD
                     SKHLKYVGASAIHVVAPGAKVTCPGIVPTGAYVRRWFSNAKTIELSAAALDNADSTNL
                     TFHAYNPSVRQDVVTWQKQGGAQNAILNKWSTGQNAVLEVDTLQGGSADAITFDAEGN
                     CYPWRVIIHNGKILSAVNFKYADIEFADNPSGVRGLPNSAVSVTSSSKSSTVTVPGGE
                     NTAYFKTFGPIAAGGGLIKKGAGALVAKLGDDLFGSLEVAEGSVSFTNAVDTAKIYEL
                     GSLTLGAGTVFNVDGGVTLNVKMISVGTGARITGTGTIAFPNLSMIPEGVIASSVKIG
                     IAGVSGGLVHGEIVPAVVGDPAFWLDTAAADSLVTEENGAVTNVVRWNDCRKASPDDG
                     YMFATNCWAKPTLANMLIQNYTHRSEGARINSFNRNYVRIVANPASLAHCEALVWSRK
                     LTGIKSVFLVMSAADGGGCVLGNTAKLGDFGRGATGQAAIFYSGTDASPEKVYKAPMY
                     INGVKVVNHFTFGPYTGFPNNWRPFLFEVHPTADGGEADAFGMQGTNRLDLAGGQRIF
                     ECILYTNELTRTEQAQVADYLTRKWFNACYGGAYALDEAEETIDATTFDRLDIVSGDY
                     AVEAITGTSFVKTGAGGMYVASLTGGTDLTIPEGSMVISSAKAASFANLPVDDSGVYM
                     HLDANNLEGSTMSEIDGKGECVKIWKSLRGATLRTATNFSTNHPALKTTGGSARMKDG
                     MKYVDLGEFREVPTTTGYGTVNANCGETYHFMKSDGIRAMFMVFGGTGPLVGNNGIYQ
                     NYGEGGLYRGDNAAA"
     gene            3172..4905
                     /locus_tag="PLZ74_08030"
     CDS             3172..4905
                     /locus_tag="PLZ74_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOR98309.1"
                     /translation="MKMKNRAFVFGVVGVVAAWGSCREPMTFCNPLDLDYGFRNELGF
                     HEGADPVCLTYKGDYYLFASKSGGYWWSPDFVCWRKVSPPNLPLANYAPTAMEYRGEL
                     YFTASGAGIFKSSRPKDEDSWELVNAQKSNVDPALFADDDGRVFLYAGCAAGKRRGAP
                     TVIELDPANQFKRIGAAAECVRPLESERGWEVRGNTNQGHGLDRSDKEPWIEGSWMNK
                     HGGKYYYQYAAPGTEFDTYGDGVCVSRSPMGPFVYEPYSPFSFKPTGFIPGAGHGATF
                     QDKHQRLWHVATGVIRGVERRVVLFPARIDAAGRLFAETAFGDLPQYLPGKNPSPGKG
                     NLVGWMELGCGKPVSASSEHSGARLARCAFDTSIRSWWQPKGSDSNAWIGVDLGAIKR
                     VCAIQVHFAETEEVRRSTNSLPQDFVQKYRVDCSDDGVHWVTRVDQSQNPKDRMNAYH
                     ELENPVATRHLRLINCGTKSGYFAVAGLRVFGTGEGTPPAPASNVRVIRGKADRRHVT
                     VEWEGGAGAKGAVVRYGVAADAMFTPFEIRGQTRLDIYSLNAGAPYWFTVDTFNENGI
                     TRMRADPVFVE"
     gene            4965..7232
                     /locus_tag="PLZ74_08035"
     CDS             4965..7232
                     /locus_tag="PLZ74_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98310.1"
                     /translation="MRHMLFVALGLLPLAVSAATGGDPLKGLVCGTGDACPQGYVPPL
                     LGNGELSMTVDWTCGMRERSYAGIRPAIYWAGRRLPPPDGGLFEFGKFNHRVIIGGVL
                     RDYPDVWEQELDVRRATLFSRNSYTNGLRIESEIIVPLTRNLIAFRRTLVSTATAPLE
                     VTLDYIFQAPKHPRLTGTWAWRKETRYAVYSCRTYGNDIRDAMIAAYKGADEPAHYTV
                     KNDRDATLSATFTLQPGERKVTTFFIQYEDTLDSGKLADRIQNTRQWVWDNGFNALLR
                     EHEQAWAAYHGESSIEVPEPKIQRMIDVAQYHLRVNATRWSFPVGIFPGHWHGKYFGW
                     DEMFCHQGLLLANHVAVARRCPEFRKALLKTACARVSHYSRKGKYGARYLWESYEDGR
                     VEGAPQGFWLDHIFHMSNIAKSVWTQYLYSGDLEYLKEVGYPVLIECARYYRSHWVYE
                     DSDGTWYIGKCTDLERLGPARERPFMTTCGAIYTLRAAADAAERLHMNLDEAADFRTT
                     ADKLVASLPHRDGAYIAYKNCTVPSVATLGGLFPYPIFDKSNDLQREAAYRFIRDGRL
                     AGNMYPVGKQICPWYAGKMAVAMTLLEDNVEPVKLLREAAGVTGLFGETFEINEPGTV
                     VAHPWFSTASGNVLVALCHMLLHNAGDELRLGIGVPDEWKNYAFRLPAHGGLLADACV
                     KDGRLATLRLTPNATVKKPQSFTLVFREGMLPAAGLSASCVKTQTREDGKVYVALAVN
                     GPVTLLEAGKNQKGL"
BASE COUNT         1431 a         2124 c         2295 g         1493 t
ORIGIN      
        1 tgcggccgcg ttgtcaccgc ggtagaggcc gccctcccca taattttgat aaatgccatt
       61 attccctaca aggggaccgg tgccgccgaa caccatgaac atcgcacgga tgccgtcgga
      121 cttcatgaag tgatacgtct caccgcaatt ggcgttcacg gtaccgtatc cggttgtcgt
      181 cggcacttcc cggaattcgc cgaggtcaac gtacttcatg ccgtctttca tgcgcgcgga
      241 gccgcctgtc gtcttcagcg ccgggtgatt ggtggagaaa ttcgttgccg tgcggagcgt
      301 ggcgccgcgg agactcttcc agattttcac acattcaccc ttgccgtcga tctcgctcat
      361 tgtggaacct tcgaggttgt tcgcgtcgag gtgcatgtag acgccgctgt cgtccaccgg
      421 caggttcgcg aagctcgccg ccttcgcgga ggagatcacc atcgaacctt ccgggattgt
      481 gagatccgtg ccgccggtga gcgaggcgac atacatcccg cccgcgccgg ttttcacgaa
      541 agaggtgccg gtaatcgcct ccaccgcgta atcgccggac acgatgtcga gacggtcgaa
      601 cgtcgtcgcg tcgattgtct cctccgcctc gtccagcgcg tacgcgccgc cgtagcaggc
      661 gttgaaccac ttgcgcgtga ggtagtcggc cacctgcgcc tgctcggtgc gggtgagctc
      721 gttcgtgtag aggatacact cgaagatccg ctgtccgccc gcgagatcga gccggttggt
      781 cccctgcatg ccgaaggcgt ccgcctcgcc gccgtccgcc gtcggatgca cctcgaagag
      841 gaaggggcgc cagttgttcg ggaaacccgt gtacggaccg aacgtgaaat ggttgacgac
      901 cttgacgccg tttatgtaca tcggcgcctt gtacaccttc tccggcgagg catcggttcc
      961 ggaatagaaa atggcggcct gccccgtggc gccgcgcccg aagtcgccga gcttggccgt
     1021 gttgccgaga acgcatccgc cgccgtccgc cgcgctcatc acgaggaaaa cggacttgat
     1081 ccccgtcagc ttccggctcc acacgagcgc ctcgcagtgg gccagcgagg cgggattcgc
     1141 gacgatcctc acgtagttgc ggttgaaact gttgatccgg gctccctctg accggtgagt
     1201 gtaattctga atcagcatgt tcgcgagcgt tggttttgcc cagcagttcg tcgcgaacat
     1261 gtagccgtca tccggcgatg ccttgcggca gtcgttccag cgcacgacgt tcgtcacagc
     1321 gccgttctcc tctgtgacaa gcgaatctgc cgcagccgtg tccagccaaa acgccggatc
     1381 gcccaccacg gcggggacga tctcgccgtg tacgagaccg ccgctgacgc ccgcgatccc
     1441 gatcttcacc gaagaggcga tcacgccctc ggggatcatg gagaggttgg ggaaggcaat
     1501 cgtgcccgtg ccggtaatcc gcgcccccgt gccgacggaa atcatcttca cgttaagcgt
     1561 cacgcccccg tcgacgttaa agaccgtccc tgcgccgagg gtcaatgagc cgagctcata
     1621 gatcttggcg gtgtcgaccg cgttggtgaa ggagaccgaa ccttccgcga cttcaagcga
     1681 accaaaaaga tcgtcgccga gtttcgcgac gagcgcgccc gccccctttt tgatgagccc
     1741 tccgcctgcg gcaatggggc cgaatgtctt gaaataggcc gtgttctccc cgcccggcac
     1801 ggtgacggtc gaggacttgg acgaggaggt gacgctgacg gcggaattcg gaagaccgcg
     1861 cacaccggag ggattgtcgg cgaattcgat atccgcatac ttgaaattaa ccgcactcaa
     1921 gatcttgcca ttgtggataa tgaccctcca gggatagcag ttcccctccg cgtcgaacgt
     1981 aatcgcgtcc gcgctgccgc cctgcaacgt atcaacctcc agaaccgcat tttgcccggt
     2041 gctccacttg ttgagaatcg cattttgggc tcccccctgc ttctgccagg tcacgacgtc
     2101 ttggcgaacg gacggattgt aggcatggaa ggtcaagttg gtcgaatcgg cgttgtccag
     2161 cgccgccgca gaaagttcga tcgtcttcgc gttagagaac cagcggcgga cgtaggcacc
     2221 cgtcggcacg atccccgggc aggtcacctt cgcgcccggc gccaccacgt ggatcgcaga
     2281 agcgccgaca tacttcaggt gctttgaatc cttcgtcgcc ttccaaagcc cggtcacctc
     2341 gccacactgg cgggacgcat agagatggag gttggcagag ccgtgcgtcc agaagttggt
     2401 cggcagggcg ttgaggatcg tctcggaatc ggaattggcg tagcagtggt gatggtgctc
     2461 gacctcgccg ccggcgaaca ccatcttgcc gctgatcgca ttgacgacat cgcccgcgaa
     2521 ggcgccgcac acactggaga tatcgttccc gaaggcgtgg gccgtcgcgt agagcaccga
     2581 ggcgtagccg aggtagtcgt gatagtggag tgtgccgagg ctgacgttct cgccgatgaa
     2641 gttgatgatc ttcccgccgc cggcacgcag cgtcaaaagc gtgaggttat tcggcaacgc
     2701 cacgtacatc tgctcggtga gcgtgcccat cgtcgccttc gcgaacttcg cctcggcggt
     2761 tgtgccggac ggcatgcccg ccggatccca gtacgaggtc accgaaaagt ccaccggatt
     2821 ctcaggcgaa cttccggtga tcggcccctt gtagagactc acggaggttt gcgcctgagc
     2881 acccatctgg aaacagaacg ccgccacggc aaatgtccaa gcggagatga aacaagaatc
     2941 aaagctcagc ctgcttttca tacaagactc ctcggaggtg attttcacaa aaccctttta
     3001 tcaggatcat taaaattgtg cgcgattttg gcgttggcgt caatatcgtt cgtaagatta
     3061 agggtgatcc ggactatccg acgatcaagg aatccattgg acgaactgac ccggtacgtt
     3121 gtatcctgtc ttcatcggca tcgagggcgc agccccgggg cattttttaa tatgaagatg
     3181 aagaacaggg cttttgtgtt tggggtggtc ggcgtggtcg cggcatgggg atcttgccgg
     3241 gagcccatga cgttctgcaa cccgcttgat ctcgattacg gcttccgaaa cgagctcggg
     3301 tttcacgaag gcgcggatcc cgtctgtctg acgtataagg gagattatta cctgttcgcc
     3361 tcaaaatcgg gtggctactg gtggtcgcct gattttgtct gctggcggaa agtgtcgccg
     3421 cccaacctgc ctctggcgaa ctatgcgccc acggccatgg aatatcgggg ggagctttat
     3481 ttcacggctt cgggtgcggg tatcttcaag agttcgcggc ccaaggatga ggactcatgg
     3541 gagttagtca acgcgcaaaa aagcaatgtg gaccccgccc tttttgccga tgacgacggt
     3601 cgcgtgtttc tttacgccgg ttgcgccgcc ggaaaaagac gcggggcgcc taccgtcatc
     3661 gagcttgatc cggccaacca gttcaagcgt atcggtgccg cggcggaatg cgttcggccg
     3721 cttgagagcg aacgcggttg ggaggtgcgg ggcaacacca atcagggaca tggtctcgac
     3781 cgctccgaca aggagccgtg gatcgagggg tcctggatga ataaacacgg ggggaagtat
     3841 tactaccaat acgcggcacc cggtaccgag tttgacacgt atggtgatgg cgtttgcgtt
     3901 tcccgttcgc cgatgggacc gttcgtgtat gaaccgtaca gtcctttctc gttcaagccg
     3961 acggggttta ttcccggtgc cggccacggg gcgacctttc aagacaagca ccaacgcctt
     4021 tggcatgtcg ccaccggtgt gattcgcggt gtcgagcgtc gtgtggtcct ttttcccgcc
     4081 cgcatcgatg cggcaggacg gttgtttgcg gaaacggcct ttggtgacct gccgcagtat
     4141 ctgcccggca aaaatcccag ccccgggaaa gggaatcttg tcggctggat ggaactgggt
     4201 tgcgggaagc ccgtcagcgc ttcgtcggaa catagcggcg cccgtctcgc ccgatgcgcc
     4261 ttcgacacga gtatccgatc ctggtggcag cccaagggat cggactcgaa cgcttggatc
     4321 ggtgtcgatc tcggcgcgat taaacgggtg tgcgcgatac aggttcattt cgcggagacg
     4381 gaagaggtgc ggaggagcac aaacagtttg ccgcaggatt ttgttcaaaa atatcgggtg
     4441 gactgctccg acgacggcgt gcactgggta accagggtcg accaatccca aaacccgaag
     4501 gaccggatga acgcttatca cgagctggaa aatcctgtgg ccacgcggca ccttcggctt
     4561 ataaattgcg gtacgaagtc aggatacttt gctgttgcgg gtcttcgcgt tttcggaacc
     4621 ggagagggaa cgcctcccgc gccggcaagc aacgttcggg tgattcgcgg gaaggccgac
     4681 cggcggcatg tgacggtgga atgggagggc ggggcaggcg ccaaaggcgc ggtcgtacgt
     4741 tacggtgtgg cagcggatgc gatgttcacg ccctttgaaa tccgcggtca aacgcggctc
     4801 gacatttaca gcctgaatgc gggggcgccc tattggttca cggtggatac gtttaatgag
     4861 aacgggatca cccgcatgcg tgcggaccct gtttttgttg agtgagggaa gaggggggag
     4921 acaggagaca aaagacgaag gcattagtca tgggaacgca aaggatgcgg catatgttgt
     4981 ttgtggcgct gggcctcctg cctttggcgg tctcggccgc gacagggggc gatccgttaa
     5041 aggggctggt ctgtggcacg ggggatgctt gtccgcaggg ctacgtgccc ccgctgttgg
     5101 gtaacggtga gttgagcatg accgttgact ggacgtgcgg tatgcgcgag cgcagctatg
     5161 ccgggatcag accggccata tactgggctg gccggagatt gccgcccccc gatggcggat
     5221 tgttcgagtt cggcaagttc aaccaccgcg ttatcatcgg cggggtattg cgtgactatc
     5281 cggatgtctg ggaacaggag ctggatgtcc ggcgcgcgac actgttcagc cgcaacagtt
     5341 acacgaacgg cctccgcatc gagagcgaga tcatcgtgcc gcttacccgt aacctgatcg
     5401 ccttccgccg cacattggtc tccacggcaa ccgcgccgct ggaggtcacg ttggactaca
     5461 tctttcaggc gcccaaacat ccgcgcctga ccggaacgtg ggcgtggcgt aaagaaacgc
     5521 gttacgccgt ctattcctgc cggacttacg ggaatgacat ccgtgatgcg atgatcgcgg
     5581 cctataaagg agcggacgaa ccggcacact acacggtcaa gaacgaccgg gacgcaacgc
     5641 tttccgccac atttaccctt cagcccggtg agcggaaagt caccacgttc ttcattcagt
     5701 atgaagacac gctagattcc ggcaagcttg ccgaccgcat ccaaaacaca cgccagtggg
     5761 tttgggacaa cgggttcaac gccctgttgc gcgagcatga gcaggcttgg gccgcatatc
     5821 acggcgaatc gtccattgag gtgccggagc cgaaaatcca gcgtatgatc gatgtcgcgc
     5881 agtaccacct gcgcgtcaat gccacgcgct ggtctttccc cgtggggatc ttcccggggc
     5941 attggcatgg gaaatacttc ggatgggacg agatgttctg ccatcagggg ttgttgctcg
     6001 ccaatcacgt cgccgtggcc cgccggtgtc cggagttccg taaagccctg ttgaaaaccg
     6061 catgcgcccg tgtgagccat tacagcagaa aaggcaagta tggcgcccgc tatctatggg
     6121 aatcgtatga ggacgggcgc gtggagggag cgccgcaggg cttctggctg gaccacatct
     6181 tccacatgtc caatatcgcg aaaagcgttt ggacgcagta cctttacagt ggggatttag
     6241 aatacctcaa ggaagtcggg tatccggttc tgatcgagtg tgcccgctat taccgctcgc
     6301 actgggtgta tgaggattcc gacggcacgt ggtacatcgg caagtgcact gatctggagc
     6361 gccttggccc ggcgcgcgag cgaccgttca tgaccacctg cggcgcgatc tacaccttgc
     6421 gggccgcagc ggatgcggct gaacggcttc acatgaatct ggatgaggcg gcggatttca
     6481 gaacaacggc tgacaagttg gttgcctcgc tgccgcatcg cgacggagct tacatcgctt
     6541 acaaaaactg cacggttccc agcgtggcca ccttgggagg gctcttcccg tatccgatct
     6601 ttgacaaaag caacgacttg cagcgtgaag cggcctaccg cttcattcgg gacggccgcc
     6661 tcgctggcaa catgtatccg gtcggcaaac aaatctgtcc ttggtatgcg ggcaagatgg
     6721 cggttgccat gaccctgttg gaagacaacg tggagccggt caaactgctg cgcgaggccg
     6781 ctggcgtgac gggattgttc ggcgagacct tcgagatcaa cgagcccggc acggttgtcg
     6841 cgcatccctg gttctcgacc gcctcgggca atgtgcttgt cgccctgtgc cacatgctcc
     6901 tgcataacgc cggggatgag ttgcggcttg gcatcggtgt gccggacgag tggaagaatt
     6961 acgccttccg gctgccggcg catggaggcc tccttgccga cgcctgtgtc aaggacggcc
     7021 gactcgcgac gttgcgcctg acaccgaacg cgacggtcaa gaaaccccag tccttcacgc
     7081 tcgtcttccg ggaggggatg ctgccggcgg cggggttatc cgcctcgtgc gtgaagacgc
     7141 agacacggga agacggcaag gtctatgtgg cccttgcggt gaacggcccc gtcacgcttc
     7201 ttgaagccgg aaaaaaccag aaggggttat aacgaaaaga acgggttggc tgaaagcgtt
     7261 ccgctccttg ttgatgatct gtgccgagcc gtattgagat gcgggcggag cccgcaatct
     7321 caaaggctgt gaggctgtga ggc
//
LOCUS       DAOSRB010000141         7333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9459_length_7333_cov_7.031739, whole genome shotgun sequence.
ACCESSION   DAOSRB010000141 DAOSRB010000000
VERSION     DAOSRB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9459_length_7333_cov_7.031739"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1372)
                     /locus_tag="PLZ74_08040"
     CDS             complement(134..1372)
                     /locus_tag="PLZ74_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98311.1"
                     /translation="MNRCWVLLGLIVVLGHAANGAIEIACDYPGGNVRVIDIDEKSGV
                     VKVAPDLRDTVGNWFHFDFTVTGADGKKLSFRFPEGGFPYISSLGPAISADGGRSWRW
                     LNQDGRRHEPANRFDYVFDEKESSVRFAMSIPYSQKDWDDFMKAYRENPDVKFETLCK
                     SQSGQRDVELVRVPCRGKARYLMVLTARHHACETTGNPPMEGAFAELMSGSKDGNWLR
                     DHADCVFVPFMDKDGVEAGDQGKNRKPWDYNRDYLKGRYTSVRALKDLIVRDSVGKRI
                     VFLDLHSPFVRSFKECPEQDQVFSFGLTHALQNARWNDFRRNWIETQKGGGLRYTGAY
                     DIQAGTGHAAITEKAVREGFAGSHHWVSMLENCCLSTCVEFGYSLCGGVNSIAGMREL
                     GMNMMKAVARTVSIEPKRSN"
     gene            1623..5354
                     /gene="smc"
                     /locus_tag="PLZ74_08045"
     CDS             1623..5354
                     /gene="smc"
                     /locus_tag="PLZ74_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOR98312.1"
                     /translation="MYLKQIELIGFKSFAEKTRLQFEPGMIAIVGPNGCGKSNISDAI
                     RWVLGEQRPTALRCAKMADVVFNGTDSRKPLGMAEVSITFADCEGFLETEFNEVTVTR
                     RVFRSGEGQYFINKTPCRLKDVQRLFMGTGIGTTSYSVMAQGQIDIILSSRPEDRRIV
                     FEEAAGITKFKADRKEAMRKIDQTDANLLRLADVIREVKRQIGTLQRQAGKAQKYKEL
                     RDELRGLDIFLTRRRLAALDIRIRELDTTIHALHEQWSSHQEFVAEAEAESSRIHGLI
                     HETEERIANLTEQATQADNRYLRAQEVIKVNEQRIAEYRAWAERDNREISETRTQIEQ
                     LRLQQESLTQKRLLLQQNEEVEQKALKEAQTQFDAHRLQIEQTRAQLQQDRQKSVECE
                     RRTAQIQQELADMEARQRELLMKRDRLAAEHGQMQETLAATEQACAEVQARLDTLTAE
                     VAATDERFEARNAEQAAVAEELNTLQAESARLQSEAAAKRAQIELLKDAGEARDDYAP
                     GSQLLLDPANPLGLEPQTVLGPLAEMFNAPPDLRLALEAALRAWLDAVIVRDAESARQ
                     IFALLLARGTPAAARVIAASSQGLSGGVDPDAPLSPPSPGLTPLLDRVTVEEPFSEAA
                     RRLLGTVFLAESLDAVPAVLPAGCRVVTRDGVIAHADGCAELWMPDSPVSSPLARKLL
                     AAEAADQLAVLETTFRTTRTRQEQLAARSAELSLNLTQMRKTLDENRRRAAQAEGEHQ
                     SVSRDAERARARLDHVSRELGALNEQTSGDEEIRAAHTNELDELTVMRASLLERITEN
                     STLLQEREGVFSELSHTLTECRIRVSSVSQQIEHAVGQREAIQTRMDELERTVQGRNR
                     GVMSYDESIERLAQEIASLEANLDPMRITAETLHQKIEETRRERAARQSDLEKTDATL
                     TERRRELETVREQRSRAEIDLAESRIRRQNHLDHVYNEYGLSPEELIAHADPAWKEGG
                     PPPISEIEARTTSLNADIQALGPVNLVAIEEHKELEERYSFLKAQEEDLLKSKEQILE
                     LIAMINKKSSEMFQSTFDQANAHFSTMFTRLFNGGQAKLVLLENAEDPLECGIDIIAR
                     PPGKRPQSITLLSGGERTMTAVSLLFAIFMIKPAPFCMLDELDAALDDSNIGRFVQTL
                     KDFLKHSQFLIITHNQHTIASSDIVYGVTQQEKGISKIVSMRLKEIGIKPLELGVPED
                     AANAVSAVEEPPPRSRRRGRKKKSGDDAAPDPEVSAPAE"
     gene            complement(5500..7170)
                     /locus_tag="PLZ74_08050"
     CDS             complement(5500..7170)
                     /locus_tag="PLZ74_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR98313.1"
                     /translation="MREAWLNQTTFNSYEAFYREFHLNVPECFNFAFDVVDAIAAEDP
                     ERRAMVWCDDQGSERVFTMSDIARASNQAARAFATAGIRRGDAVMLILKRRYEYWFAL
                     MGLCRLGAVAIPATNQLTQKDVVYRIRSAGVSMILCVNEPAIRAAVDAAVTEAPVRVR
                     ALVNGGDDGTGWMDFNAACDQASPEPLEAALCARDTGACDPMLLYFTSGTTGMPKMVQ
                     HDFLYPFGHVVTARYWQGCTPGGLHLTLAETGWAKAAWGKIYGQWMCGCAVMVYDMDR
                     FHAEAVLRIVERYRVTTFCAPPTIYRYLIKEDLKRCDLSALTRCVVAGEPLNPEVYQQ
                     FLDATGLKLYEGYGQTEVTVAAGTFPWMKPKPGSMGCAAPGYDLDLIRHDGRPCEVGE
                     EGQLVIRTERSKPLGMFNGYYRDPELTHQMWRDGVYYTGDMAWRDEDGYFWFVGRADD
                     VIKSSGYRIGPFEVESALLEHPAVLECAVTGVPDPDRGQVVKATVVLAKGFEASDALA
                     SALQEHVKRITAPYKYPRRVEFVSELPKTISGKIRRVQIREQDQRNAE"
     gene            complement(7186..7332)
                     /locus_tag="PLZ74_08055"
     CDS             complement(7186..7332)
                     /locus_tag="PLZ74_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR98314.1"
                     /translation="MNGRLRVSVGGRETVLEPGDSIYFDASVPHGMRAEDGVEATFLA
                     VITA"
BASE COUNT         1599 a         2385 c         2032 g         1317 t
ORIGIN      
        1 accacctcac agcctaacag cctcacagtc tcacagcctt tgagattgcg ggctctgccc
       61 gcatctcaat acggtgcggc ggggttctca acaaaaccca atgtttacgg ggctttgcta
      121 gaattctacc cgttcaattg ctccttttgg gttcaatgga taccgtccgc gccacagcct
      181 tcatcatgtt catgccgagt tcgcgcatac cggcgatcga gttcacgccg ccgcagagcg
      241 aatagccaaa ctcgacgcat gtcgagaggc agcaattttc aagcatcgag acccaatgat
      301 gcgaaccggc gaacccttcg cgaacggcct tctcggttat agccgcatgg ccggtgccag
      361 cctgaatgtc gtaggcgccg gtatacctca gtcccccgcc cttctgcgtc tcgatccagt
      421 tgcgccggaa gtcgttccag cgggcgttct gtaacgcatg ggtgaggccg aatgagaata
      481 cctgatcctg ctcgggacac tccttgaagc tccgcacgaa cggcgaatgg agatcgagaa
      541 agacaatgcg tttgccgaca gaatccctga caatcagatc cttaagcgcc cgcacggacg
      601 tgtagcgccc cttcaaatag tcccgattgt aatcccacgg cttgcgattc ttgccttggt
      661 cgcccgcctc gacaccgtcc ttgtccatga agggcacgaa tacgcagtcg gcatgatcgc
      721 gcagccagtt cccgtctttg gatccggaca tcagttcggc aaacgctccc tccatcggcg
      781 gattccccgt ggtctcgcag gcatggtggc gcgcggtcag caccatcaga tagcgggcct
      841 ttccgcgaca gggaacccgc accaattcaa catcccgttg accactctgc gacttgcaaa
      901 gcgtctcgaa cttcacgtcc ggattctcgc gataggcctt catgaagtca tcccagtcct
      961 tctgggaata cggaatcgac atcgcgaaac gcaccgacga ttctttttcg tcgaagacgt
     1021 aatcgaacct gtttgccggc tcatgtcgcc gtccgtcttg gttcaaccag cgccaactgc
     1081 gtccaccatc agcgctgatc gccggtccca gtgaggagat gtacgggaag ccaccttccg
     1141 gaaagcgaaa actcagcttc ttcccatccg cgcccgtcac ggtgaaatcg aaatggaacc
     1201 aattgcccac ggtgtcccgc aagtcaggag cgaccttcac cacgccggac ttctcgtcga
     1261 tgtcaatgac gcgcacgttc ccgccgggat agtcgcacgc aatctcaatc gccccgttcg
     1321 ccgcatgtcc aagcacaacg atgagaccca acagtaccca acacctattc acgtcactcc
     1381 ttcctctatt attaaaacag acggaccttg acgtccacaa ttttcctcga caatcaatta
     1441 agcaagaaaa tgaacagtat tgcaatttaa tccgaaaaag caagcgttgg acacacctct
     1501 cccaacaagc aggtgaaaaa ctgctttagg ctttgaatcc ggccccctgt tttgatataa
     1561 gtacctcttt ctgtgacggc tgacaggcat cccgccgtca atctggctcc ctgaggtttt
     1621 tcgtgtatct aaaacagatt gaactgattg gtttcaagag ctttgcggaa aagacccgtc
     1681 tgcagttcga acccggcatg atcgcgatcg ttggacccaa cggctgcggc aaaagtaata
     1741 tctcggacgc gatccgctgg gtgctgggtg aacagcgtcc caccgccttg cgttgcgcaa
     1801 aaatggcgga tgtggtgttc aacggcacgg attcgcgcaa gccgctcggc atggcggagg
     1861 tctccatcac cttcgcggat tgcgaagggt tcctcgaaac cgaattcaac gaggtgactg
     1921 tcacacgccg cgtcttccgg tccggagaag gccagtactt catcaacaag accccctgcc
     1981 gcctgaaaga cgttcaacgg ctcttcatgg ggacgggcat tggcacgacc tcctattcgg
     2041 tgatggccca gggacaaatc gacatcatcc tctcctcgcg gccggaagac cgccgcatcg
     2101 ttttcgagga ggcggccggc atcacgaaat tcaaggctga ccgcaaagag gcgatgcgca
     2161 agattgatca gaccgatgcc aacctgctgc ggctcgccga tgtaatccgg gaggtgaagc
     2221 gccagatcgg cacgcttcag cgccaggccg gtaaagccca aaaatacaaa gagctgcgcg
     2281 atgaattgcg cggcctcgac attttcctga cgcgtcggcg tctcgccgcg cttgatatcc
     2341 gcatccgtga gttggacacc acgatccatg cgttgcacga gcagtggagt tcacatcagg
     2401 agttcgtggc ggaggccgag gcggagtcct cccgaatcca cggattgatc catgagaccg
     2461 aagagcggat cgcgaacctg acggaacaag cgacacaggc cgacaaccgt tacctccgcg
     2521 cccaggaagt catcaaggtc aacgagcagc gcatcgccga ataccgcgct tgggcggaac
     2581 gcgacaaccg cgagatttcc gagacccgga cgcagatcga acaactccgc ttgcagcagg
     2641 aatcgctcac gcaaaaacgc ttgctcttgc agcagaatga ggaagtcgaa cagaaagctc
     2701 taaaagaggc gcaaacccaa ttcgacgcgc accggcttca gatcgaacaa acgcgcgcac
     2761 agctccagca ggatcggcag aagtcggtag aatgcgaacg ccgcaccgcc cagatccagc
     2821 aggaactggc ggacatggag gcgcggcaac gcgagctgct catgaagcgg gaccgccttg
     2881 ccgccgaaca cggccagatg caagagacgt tggcggccac ggaacaagct tgcgctgaag
     2941 tccaggctcg tctcgacacg ttgacggccg aggtcgccgc aacagacgaa cggtttgaag
     3001 cacgcaacgc tgaacaggcg gccgtagccg aagaactgaa cacgctgcaa gccgaatccg
     3061 cgcgcctcca atccgaggct gcggccaagc gggcgcagat cgagttgcta aaagacgcgg
     3121 gcgaggcccg cgacgactat gcgccaggct cgcaactctt gctggacccc gccaatccgc
     3181 tgggcctcga accacaaacc gtgctcggcc cgctggcaga gatgttcaac gcgccgccgg
     3241 acctgcgcct tgcgctggaa gccgccctgc gcgcttggct cgatgccgtg atcgtgcgcg
     3301 acgccgagtc cgcgcgccag atcttcgcgc tgctgctcgc acgcggtacc cccgcagccg
     3361 cgcgtgtcat cgcggcttcg tcccaaggcc tatccggcgg cgtggatccg gatgcgccgt
     3421 tgtctccgcc cagcccgggc ctcacgccac tgctggatcg cgtcaccgtt gaagaaccgt
     3481 tttccgaagc ggcgcgccgc ctgttgggca ccgtcttttt ggcggagtcg ctggatgccg
     3541 tacctgcggt tctgcccgcc gggtgccgtg tcgtcacgcg tgatggcgtg atcgcgcatg
     3601 ccgacggatg tgcggagttg tggatgccgg acagcccggt ctccagtccg ctcgcccgca
     3661 aactgctcgc cgcagaagct gccgaccagc tcgctgtgct ggaaacaacg ttcaggacca
     3721 cccggacccg ccaagagcag ttggcggcgc gttcggctga actgagcctc aacctgacgc
     3781 agatgcggaa aacgctggat gaaaaccggc gccgcgccgc gcaggcggaa ggtgagcatc
     3841 agagcgtttc ccgtgacgcg gaacgggccc gcgcccgtct ggatcacgta tcccgtgagt
     3901 tgggcgcact caacgagcag acttccgggg acgaggaaat ccgtgccgcg cacacaaacg
     3961 aattggacga actcacggtg atgcgcgcaa gcttgttgga acgcattacc gaaaactcga
     4021 ccctgctgca ggaacgggag ggcgttttta gcgaactcag ccacaccctc accgagtgcc
     4081 ggatccgagt ttccagcgtg tcgcagcaga tcgagcacgc ggtgggccag agagaagcca
     4141 tccagacacg gatggacgag ttggagcgca cggtgcaggg ccggaaccgc ggcgtgatgt
     4201 catacgatga aagcatcgag cgcctcgcgc aggagatcgc ctccctggaa gcgaacctcg
     4261 accccatgcg gatcacggcc gaaacgcttc atcaaaagat cgaggagacg cgccgcgaac
     4321 gcgccgcccg ccagagtgat ctggagaaga cggacgccac cttgaccgag cgccgccgcg
     4381 aacttgaaac tgtgcgcgaa caacgcagcc gcgcggagat tgatctcgcc gaatcgcgga
     4441 tacgacgcca gaaccatctg gaccacgtct acaacgaata cggactctcg cccgaggaac
     4501 tgatcgccca tgcggatccc gcctggaagg agggcggacc gccgccgatc tccgaaatcg
     4561 aggcgcgcac gacttccctc aatgccgaca ttcaggcgct cggccctgta aatctggtgg
     4621 ccatcgagga acacaaggaa ctggaggaac gttactcctt cctcaaggct caggaggagg
     4681 atctcctcaa gtccaaagag cagattctgg aactgatcgc catgatcaac aagaagtcga
     4741 gcgagatgtt ccaatccacc ttcgatcagg ccaacgccca tttcagcacg atgttcactc
     4801 ggctcttcaa tggtggccaa gcaaaactcg tgttgctgga aaacgccgaa gacccgcttg
     4861 aatgcggtat cgacatcatc gcccgtccgc ccggcaaacg accacagtcg atcaccctgc
     4921 tctcgggcgg cgaacgcacc atgaccgcgg tgagtctgct cttcgcgatt ttcatgatca
     4981 aaccggcccc gttctgcatg ctcgacgaac tggacgccgc gctggatgac agcaacatcg
     5041 gacgtttcgt tcagaccctg aaagacttct tgaaacactc gcagttcctg atcatcacgc
     5101 acaaccagca caccatcgcc agttcggata tcgtttacgg cgtgacacag caggagaagg
     5161 gcatctcgaa gatcgtctcg atgcgtctca aggagatcgg gatcaaaccg ctggaactgg
     5221 gcgtccccga ggatgcggcg aacgcggttt cggccgtaga agagccgccg ccgcggagtc
     5281 ggcggcgcgg ccgcaaaaag aagagcgggg acgacgccgc ccccgacccc gaagtcagcg
     5341 cgccagccga ataatcccgg caacctcttg tgcgccgaga tcgaggactg cctcggccat
     5401 gcgctccgtg agcgccgcgc gcccttccat aatctcccac ggatcgcccg cgccatagcg
     5461 gacggcaatg gcgataaact tggcggaagc ttgcgtcggc tattccgcgt tgcgttgatc
     5521 ctgttcccga atctgtacgc ggcggatctt gccgctgatc gttttcggca actccgaaac
     5581 aaactcgacg cggcgcggat atttgtatgg cgccgtgatg cgtttgacat gctcctgcaa
     5641 cgcgctagcc agcgcgtcgg aagcctcaaa tcctttggca agcacgaccg tggccttcac
     5701 cacctgaccg cggtccggat ccggcacacc cgtcaccgcg cattccagta cagcgggatg
     5761 ctccaacagc gcgctttcca cctcaaaggg tccgatgcga tacccggaac tcttgatcac
     5821 atcatccgcc cgtcctacaa accagaagta cccgtcctca tcgcgccaag ccatgtcgcc
     5881 ggtgtaatag accccgtccc gccacatctg atgcgtcagt tcaggatcgc gatagtagcc
     5941 attgaacata ccgagcggct tggagcgttc cgtgcggatc accagttgcc cttcctcgcc
     6001 cacttcgcac ggtcggccgt catgacgaat gaggtccaag tcataccccg gcgcggcaca
     6061 gcccatggac ccgggcttgg gcttcatcca cgggaaggtg cctgccgcca cggtcacctc
     6121 tgtctgaccg tacccctcat acagtttcag ccccgtggca tccaaaaact gttgatagac
     6181 ttcggggtta agcggctcgc cggccaccac gcaccgggtg agcgccgaca aatcgcagcg
     6241 tttcagatcc tctttgatga gataccggta tatggtgggc ggcgcacaga atgtggtgac
     6301 ccggtagcgt tcgacgatcc gcaataccgc ctcagcatga aaacggtcca tgtcatatac
     6361 catgaccgcg caaccgcaca tccactgacc gtagatctta ccccacgcgg ctttggccca
     6421 tccggtttcc gccagagtca ggtgcaagcc gcccggtgtg caaccctgcc aataccgggc
     6481 cgtcacaaca tgtccaaagg gatacaggaa atcgtgctgc accattttcg gcatgccggt
     6541 cgtgccggag gtaaagtaga gcagcatcgg atcacaagcc ccggtgtccc gcgcacagag
     6601 cgccgcctcc agcggctccg gcgaagcctg atcgcatgcg gcattgaaat ccatccagcc
     6661 cgtgccgtcg tccccaccat tcaccaatgc ccgtacgcgt accggcgctt ctgtgaccgc
     6721 cgcatccacc gccgcgcgga tcgcgggttc gttgacgcat aggatcatgc tgactccggc
     6781 cgagcgaatg cggtacacga catccttctg ggtcagttgg ttggtggccg ggatcgccac
     6841 ggcccccaag cggcacagtc ccatcaacgc gaaccaatac tcataccgcc gtttcaggat
     6901 cagcatcacg gcatccccgc ggcggatgcc cgctgtcgcg aacgcacggg ccgcctggtt
     6961 gctggcgcgc gcgatgtcgc tcatcgtgaa gacccgctca ctcccttgat catcgcacca
     7021 gaccatcgcg cgtcgttccg gatcctccgc cgcgatcgca tccaccacat caaaggcgaa
     7081 attgaaacat tcgggcacat tgaggtgaaa ctcccggtag aacgcctcat acgaattgaa
     7141 cgttgtctga ttgagccacg cttcccgcat acgtctcccc tccgcttatg cggtgatcac
     7201 cgccagaaac gtggcctcca caccgtcctc agcccgcatg ccgtgcggca ccgaagcgtc
     7261 aaaatagatg ctgtcccccg gttccagcac cgtttcacga ccgcccaccg aaacccgtaa
     7321 ccggccgttc agc
//
LOCUS       DAOSRB010000142         7300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9516_length_7300_cov_6.955418, whole genome shotgun sequence.
ACCESSION   DAOSRB010000142 DAOSRB010000000
VERSION     DAOSRB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9516_length_7300_cov_6.955418"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1331)
                     /gene="lpxD"
                     /locus_tag="PLZ74_08060"
     CDS             complement(288..1331)
                     /gene="lpxD"
                     /locus_tag="PLZ74_08060"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOR98315.1"
                     /translation="MILTAIELAERLGGTLTGDGSVEVRALESLFAARPGDAAFFKDA
                     KYAAQLAATKASVVLVSETWQGTCPAPAVIRVPDPDGAFARAAVWFAPSPPERAPGIH
                     PTALIGRDVHLGASVHVGPYTVIEDGCVIGDGCVIEAQVFLGRQVSMGEGCHIYPQVT
                     VREGCILGCRVVLHSGVRIGGDGYGYTPKVAADGTVTIDKIPQLGIVELGDDVEVGCN
                     ATIDRARFGRTRIGNSTKIDNLVQIGHNVQVGSCSGIIAQAGVAGSTRIGSGCLIWAQ
                     AGISGHLEIGDRGQVGPKSGLAKDVPAGEYVLGLPAIPRREFAATLLVPRQIEKLKRQ
                     LAEIQAELAALKS"
     gene            complement(1328..1924)
                     /locus_tag="PLZ74_08065"
     CDS             complement(1328..1924)
                     /locus_tag="PLZ74_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOR98316.1"
                     /translation="MKQMSGILAAVFAVTMAMAQGGTAVVNMVDLVRFHPSRERDRKL
                     MQDTEKEFQSKLDKQRDRFEQLRDDYEKAVKEARNPALNDKARAEAEDKAMKHRDVLA
                     EADRDLRQEMQKLQRDLGDLDTRLLRQVTSDIREVLSKFAQEKKYLVVLDGTTIAYFD
                     PKLDVTDEVLKRMGVDPKVRQEAKEKAEKEKAAAEKKK"
     gene            complement(1934..4297)
                     /gene="bamA"
                     /locus_tag="PLZ74_08070"
     CDS             complement(1934..4297)
                     /gene="bamA"
                     /locus_tag="PLZ74_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOR98317.1"
                     /translation="MGKVMRAWLAVAVLAAGWVLAADNTVKEVQVRVLDQLGTDASDV
                     LSVCGVAAGSKLSQEALSKDVRALLDTGRYGYAGVELEKIEGGIRVIYVVRRRYRFQE
                     PLAIQGANYLSESKIRKLTELKSGDYIDEPVLSTQVAKVRDEYVKRYFPDVKIGMAIT
                     PIQGSAGFANVTLTIEEGERAKIRAYRFTGNTSIPEADLRSSFGQRPWYDPRGWFTDT
                     PVSRQDLEDARQQAEEVYQNHGFLDAKVASPVMEKVGKEKVDVVFAVTEGDVYHVEST
                     AIRGVKLFPEREVLASSRLKAEDLASRKAIDDAAKDIRDYYGSRGYVDTIVRPMKETV
                     PGKPGRMSIAFDVREGELVYIRNIVIRGNNKTRDKVIRREVLVNPGEIMNEPRIERSE
                     NRLKNLGYFANVRHYTEQTGQPGTRDLVYEVEEQRTGNFMIGAGFSSVDNLVGFMEIT
                     QSNFDILNWPNFTGGGQKARLGIEVGSRRQTAEVSWTEPWFLDRPLALTVEAYRRMRW
                     FDEYDEIRTGGSVGLSYPVKVGRVGLRYTMELVEMDDVESGEWYLDPKGDTASTPENW
                     NGDENDYFNWQEDEYGDALNSVIRLYWAHDTRDQPFVPTKGYQTTLFGELSEGAIGDN
                     EIYKLGVNYRHWFRMPWYKHVLSLRGRFETVDAYSGDVPIYEKLFLGGPRTVRGVEYR
                     DIGPKVYRGEGSKKHAPIGGQTLAMATVEYTVPVFKAVRVATFMDVGSIGEDVFDPEL
                     SDVCVSVGVGLRIDIPGFPIRFDFAKPVVEDDSYTDDEFFSFAIGFE"
     gene            4798..6141
                     /locus_tag="PLZ74_08075"
     CDS             4798..6141
                     /locus_tag="PLZ74_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98318.1"
                     /translation="MKKKTLAVVVCLFCSILSPVYARLVDTVPMIVRADSEATVTLTF
                     ADEPLLAKPDDVQVEYICADGLDSAGVPLGYGCFQPVASVVRDNTLTVSVRFRGETAH
                     TLRVIQKSSDPKKPNVLGIARLYSLCPDFFALRPYRGNMHMHSKFSDGNKNESPALMV
                     ATCRKLGHDFAIETDHRSYAGSLDAIEAFSKLPTDMKTFPGEEVHSPGNNVHILSLGA
                     SESITDWFTKSKTEYDQAVAAEQAKLPDTIPDRFKYPIAASFAVWDKIRACGGIAVFC
                     HPYWRPAYRQYIPAVVSDYLLQTAKFDAVEVLNTDSSELGILHYHELRAQGLKIAGIG
                     VTDAHSSKHLEPAYTLILAETLDFPSLAKNIRLRNCAAVDIDPVSKRQTVIGEFRFSR
                     YALFLLHHFYPLQNEVCKREGEWLLKALEGDEQAIASLKASQGTTPALRTKYWQK"
     gene            6168..7214
                     /locus_tag="PLZ74_08080"
     CDS             6168..7214
                     /locus_tag="PLZ74_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR98319.1"
                     /translation="MLTLILPALRGLMARGDTLPRLARVQPEGGEPLLTVPPGCDDAF
                     AVLQSHVPPGTSRVTLDTGGTYEAVFTDLPGRAAGRIALVTGAAQGFGFEIACDLAAQ
                     GAWVALADINEAGVLAAAEQVNEACGRSAAVGVAVNVADPDSVRAAVAAAVTAFGGID
                     LLVSNAGVLRAGSVKTLPVKDLRFVTDVNYHGYFLMVQAVAPILALQHRAVPSRWFDI
                     IQINSKSGLTGSNKNGAYAGSKFGGIGLTQSFALELVEDGIKVNSICPGNFYDGPLWS
                     DPEKGLFKQYLDSGKVPGAKTFEDVKRFYESKVPMRRGCTTPDVMKALYYLMEQTYET
                     GQAVPVTGGQVMLA"
BASE COUNT         1395 a         2350 c         2138 g         1417 t
ORIGIN      
        1 gccgccatca ttacacttgt cgctgcgggc cactgcgcgg gcgcaacagt cgatttcgaa
       61 cccgatagcg ataccgatac cgatagcgat accgataccg atttggagaa cgtgaatgtt
      121 ggctataccc ccgtacccgc actcgttcac gtacccgtac acgatgactc ttcccccccc
      181 cgtcacgagg cgattacaaa cgcattcgta tgaaatcaag gaggaaggga cggacgcctg
      241 cgcgtccgga caagccgacg cttccttctc cctgtctgaa cagtccctca ggatttgagg
      301 gccgcgagtt cggcctgaat ttcggcaagc tgccgtttca gcttctcaat ctggcgcggc
      361 accagcaacg tggccgcgaa ctcgcgcctg ggaatcgcgg gcaggccaag cacatattcg
      421 cccgcaggca catctttggc gagcccgctc ttgggcccga cctgcccacg gtcgccgatt
      481 tccagatgcc cggagatgcc ggcctgggcc cagatcaggc accccgaacc gatccgcgtg
      541 ctgcctgcga caccggcctg cgcgatgatc ccgctgcagg aaccgacctg cacgttgtgg
      601 ccgatctgca caagattgtc gattttggtg ctgttgccga tccgcgtgcg gccgaaccgc
      661 gcgcggtcaa tggtggcgtt gcacccgacc tcgacatcat cccccaattc cacaatgcca
      721 agttggggga tcttgtcgat ggtgaccgtg ccgtccgccg ccaccttcgg ggtgtagccg
      781 tatccatccc cgccgatacg cacgccggag tgcaggacca cacggcagcc aaggatacaa
      841 ccctcgcgca ccgtcacctg cggataaatg tggcatccct cacccatcga cacctgacgt
      901 ccgaggaaca cctgcgcctc gatcacgcag ccgtcgccga tgacacagcc gtcttcgatc
      961 accgtatacg gcccgacatg aaccgaggcc cccagatgca catcacgtcc gatcagcgcg
     1021 gtgggatgga tgcccggagc gcgttcgggc gggctcggcg cgaaccagac cgcggcgcgg
     1081 gcgaaggcgc catcgggatc cgggacacgg atcaccgcag gcgcgggaca ggtgccctgc
     1141 cacgtctcgg agaccaacac cacgctcgcc ttggtggcgg cgagctgtgc cgcgtacttc
     1201 gcgtccttga agaacgcagc gtcgcccggg cgggccgcga agaggctctc cagcgcacgc
     1261 acctccacgc tgccgtcccc cgtcagcgtc ccgccgagac gttcggccag ttctatcgcc
     1321 gtcaggatca tttcttcttc tcggctgcag ccttctcctt ctcggccttt tccttggcct
     1381 cctgacgaac cttgggatcg acgcccatcc gtttgagcac ctcatcggtg acatccagtt
     1441 tggggtcgaa ataggcgatc gtggtgccgt cgagcacgac cagatacttc ttttcctgcg
     1501 cgaacttgga gagaacctcg cggatgtcgc tcgtgacctg ccgcaacagg cgggtgtcga
     1561 gatcacccaa gtcacgctgc agtttctgca tctcctggcg cagatcgcgg tccgcttcgg
     1621 cgagcacgtc ccggtgcttc atggccttat cctccgcctc ggcacgcgcc ttgtcgttga
     1681 gcgccggatt gcgggcctcc ttcaccgcct tttcgtaatc atcacggagc tgctcgaagc
     1741 ggtcgcgctg cttgtcgagc ttgctctgga actccttctc ggtgtcctgc atcaacttgc
     1801 ggtcccgctc gcggctcgga tggaaccgta cgagatcgac catgttgacc actgcggtgc
     1861 cgccctgggc catcgccatc gtgacggcga aaaccgccgc cagtatgccg ctcatctgtt
     1921 tcataccatt cctttattcg aacccgatcg cgaacgagaa gaactcgtca tccgtgtaac
     1981 tgtcatcctc caccacgggc ttggcgaagt caaaccggat ggggaagccg gggatatcga
     2041 tccgcaggcc cacacccacc gaaacacaaa cgtcggaaag ctccggatcg aacacatcct
     2101 cgccgatcga cccgacatcc atgaaggtcg ccacgcgcac cgccttgaag accggcaccg
     2161 tgtattcgac cgtggccatg gccagagtct ggccaccgat cggcgcgtgc ttcttgctgc
     2221 cttcgccgcg atagactttg gggccgatat cgcggtattc aacgccgcgc accgtgcgcg
     2281 ggccacccag aaagagtttc tcatagatcg gcacatcgcc gctgtaggca tccactgttt
     2341 cgaagcggcc gcgcaggctc agcacatgct tgtaccaagg catgcggaac cagtggcggt
     2401 aattcacgcc gagtttgtaa atctcgttgt cgccgatcgc accttcggag agttcgccga
     2461 acagggtggt ctggtatccc ttggtgggca cgaacggctg gtcgcgcgta tcgtgggccc
     2521 aatagaggcg gatcacactg ttgagcgcgt cgccgtactc gtcctcctgc cagttgaaat
     2581 aatcgttctc gtcgccgttc cagttttccg gcgtggaagc ggtgtcgccc ttcgggtcca
     2641 gataccactc gcccgattcg acgtcatcca tctccaccag ttccattgtg tagcgcagac
     2701 ccacgcggcc gactttgacc ggataggaga ggccgaccga gccgccggta cggatctcgt
     2761 cgtattcgtc aaaccagcgc atccggcggt aagcctcgac cgtcagcgcc agcgggcggt
     2821 ccaggaacca gggttccgtc caagagacct cggccgtctg ccgacgcgaa ccgacttcaa
     2881 tgcccaagcg cgccttctga ccgccgccgg tgaagttcgg ccagttgagg atgtcgaagt
     2941 tgctttgggt gatctccatg aagcccacga gattatcgac actcgaaaaa ccggcgccga
     3001 tcatgaagtt tcctgtgcgc tgctcttcca cctcatagac cagatcgcgc gtgccgggct
     3061 gtccggtttg ttccgtgtaa tggcgcacgt tggcgaaata gccgaggttt ttcaggcggt
     3121 tctcgctgcg ctcgatgcgc ggctcgttca tgatctcacc ggggttgacc agcacctcgc
     3181 ggcgaatgac cttgtcccgg gtcttgttgt tgccgcggat cacgatgttg cggatgtaga
     3241 ccaattcgcc ctcgcgcaca tcaaacgcga tcgacatgcg tccgggtttg cccggcaccg
     3301 tttccttcat gggccgcacg atcgtgtcca catacccgcg cgagccgtaa tagtcgcgga
     3361 tatccttggc cgcgtcatca atcgccttac ggctcgccaa gtcttccgcc ttcagccggg
     3421 aggaggcgag cacctcgcgt tccggaaaga gtttcacgcc gcggatcgcg gtggattcca
     3481 catggtacac gtccccttcg gtcacggcga agaccacatc caccttctct ttgccgacct
     3541 tctccatcac cggcgaagcc actttggcgt cgaggaagcc gtgattttga tagacctcct
     3601 cggcctgttg gcgcgcgtcc tcaaggtcct gccgcgaaac cggcgtgtcg gtgaaccagc
     3661 cgcgcgggtc gtaccacggc cgctgaccaa acgaggaacg caggtccgcc tccggaatgg
     3721 aggtgtttcc ggtgaaacgg tacgcgcgga tcttggcgcg ctcgccctct tcgatggtca
     3781 gggtgacatt cgcaaaaccg gcgctgccct gaatgggggt gatggccatg ccgattttga
     3841 catccggaaa gtacctcttc acatactcgt cacggacttt ggcaacctgg gtcgacaaaa
     3901 ccggttcatc aatgtaatcg ccgctcttga gttccgtgag tttgcggatt ttactctcgc
     3961 ttagatagtt cgcgccctga atggccagcg gctcctgaaa ccggtaccgg cggcgcacca
     4021 cgtagatcac gcggattccg ccctcgattt tctccaactc cacacccgcg tacccgtaac
     4081 gtccggtatc caacaacgcg cgcacatctt tggagagcgc ctcctgcgag agcttcgagc
     4141 ctgcggcaac cccgcagacc gacagcacat ctgaagcgtc cgtcccgagt tggtcgagca
     4201 cacggacctg aacctctttc accgtgttat cggccgccaa cacccaccct gcggccaaga
     4261 ccgccaccgc caaccacgcg cgcatcactt tacccatttt cgcaactcct catcccattc
     4321 aaccaaacgt ccagccccgc cgcaacggcg gactgccacc ataccggagg catgaccgca
     4381 gaaaatccgg ttcatatgcg aattggaaaa attaaacctg atcctgcggc aaaacacaag
     4441 cctgttgtcg tcggttttgg ccggatggcc gggggagcgc gaaatgttga cccgcgacgg
     4501 gcggcctgcg gtgtcgggaa cgcaggatgg ggagggcgcg aattcattcg tgcattggtg
     4561 cggaagccgg aaatgcgggc atccccgccc gctggcggat cagtcggatc agtgctctgc
     4621 caaatcaccc aaacgccgca ggccgaatgt cgcgggtcgc aagtcaacgt acacgaaaac
     4681 gaaggcgcga acgaggacgg ggacttgggg ttgactttcc aaacgtatcc cgcccataat
     4741 gtgcctcaaa tgcagacacc gggggagggg tctgcggtct cagaggaaag gtgcgtgatg
     4801 aaaaaaaaga cgcttgcggt cgttgtctgt ctgttttgct caattctgtc accggtgtat
     4861 gcccgactcg tcgacaccgt cccgatgatt gtccgcgcgg acagcgaggc caccgtgacc
     4921 ctcacctttg cggatgagcc gctgctcgcc aaaccggacg atgtgcaggt cgaatacatc
     4981 tgcgcggacg gtctggactc cgcaggcgta ccgctcggat acggctgttt ccagccggtc
     5041 gcgtccgttg tccgcgacaa cacgctcacc gtttccgtgc ggttccgcgg agagaccgcg
     5101 cacaccttgc gcgtgatcca gaaaagctcc gatccgaaaa aaccgaacgt gctggggatc
     5161 gccaggcttt actcgttgtg cccggatttc tttgccctgc ggccttaccg gggaaacatg
     5221 cacatgcaca gcaagttttc agatggcaac aagaacgagt ctcccgcact gatggtcgcc
     5281 acatgccgta agctcgggca cgacttcgcg attgagaccg atcaccgctc ttatgcggga
     5341 tcgctggatg ccatcgaagc gttttccaaa ttgcccacgg acatgaagac cttccccggc
     5401 gaagaggtac acagccccgg gaacaacgtg cacatcctct cgctcggtgc ttcggaaagc
     5461 atcacggatt ggttcacgaa gtcaaagacc gaatacgatc aggcggtcgc ggcggaacag
     5521 gccaagctgc cggacacgat tcccgatcgt ttcaagtatc cgatcgccgc ctcgttcgcg
     5581 gtttgggaca aaatccgcgc ctgcggcggc atcgccgtgt tttgccaccc ctattggcga
     5641 ccggcctacc ggcagtacat cccggcggtg gtttcggact atctgctgca gaccgcgaag
     5701 ttcgacgccg tggaggtcct gaacacggac agcagtgagt tgggaatcct ccactaccac
     5761 gaactgcgcg cgcaagggct gaagatcgcc ggtatcggtg tgactgacgc ccactcgtca
     5821 aaacatctgg aacccgccta cacgctgatt ttggcggaaa cactcgattt tccgtcgctc
     5881 gccaaaaaca tccgcctgcg caactgcgcg gcggtggata tcgatcccgt ttcgaaacgg
     5941 caaaccgtca tcggcgagtt ccggttctcc cggtacgcgc tcttcctgct ccatcatttc
     6001 tatccgttgc agaatgaggt ctgcaaaagg gagggcgaat ggctgctgaa ggcgcttgaa
     6061 ggtgacgagc aggccattgc ctcgttaaag gcatcgcagg gaacgactcc cgcgctcagg
     6121 acgaaatatt ggcagaagtg aacccgatat tgcgaaagga ccccctcatg ttgaccctga
     6181 ttctccccgc cctgcggggc ctgatggccc gcggcgatac cctgccgcgt ctggcgcgtg
     6241 tccagccgga aggcggcgag ccgctgctca ccgtgccgcc gggatgcgat gacgcgttcg
     6301 ccgtgttgca aagccatgtg ccgccaggca caagccgcgt caccctggac accggcggca
     6361 cctacgaggc ggtctttact gatcttccgg gtcgtgccgc gggacggatc gcgctggtga
     6421 ccggcgcggc ccaaggtttc ggatttgaaa tcgcatgcga tcttgctgcc caaggtgcct
     6481 gggtcgccct ggccgacatc aacgaagcgg gcgtgctcgc ggcggcggag caggtcaatg
     6541 aggcttgcgg acgttcggcg gccgtcggtg tggccgtgaa cgtggcggat cccgattcgg
     6601 tgcgcgcggc ggtggcagcg gcggtgacgg cattcggcgg aatcgacctg ctggtctcaa
     6661 acgccggcgt actgcgggcc ggcagcgtca agacgcttcc cgtcaaggac ctccgcttcg
     6721 tgacggatgt caattaccac ggttatttcc tcatggttca ggcggtggca cccattctgg
     6781 cgctacagca ccgggccgtg ccgtcacgct ggtttgacat cattcagatc aacagcaaat
     6841 ccggactgac cggttcgaac aagaacggcg cgtacgcggg atcgaagttc ggcggcattg
     6901 gcctgacgca gagtttcgcg ctggaactgg tggaggacgg catcaaggtc aactccatct
     6961 gccccggcaa cttctatgac ggaccgctct ggtctgaccc ggagaaaggt ctgttcaagc
     7021 agtacctcga cagcggcaag gttccgggcg cgaagacatt cgaggatgtc aaacggttct
     7081 acgagtccaa ggttccgatg cggcgcgggt gtaccacgcc ggacgtgatg aaggcgctgt
     7141 actatttgat ggaacagacg tacgagacgg gacaggccgt accggtcacg ggtggccagg
     7201 tgatgctcgc ctaggccgag gcggtcggaa ccagcagacc gtcgcgcaac tccagcacac
     7261 ggtcgcagag cgccgcggtc tcgggtgaat gggtcaccat
//
LOCUS       DAOSRB010000143         7262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9572_length_7262_cov_8.005273, whole genome shotgun sequence.
ACCESSION   DAOSRB010000143 DAOSRB010000000
VERSION     DAOSRB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9572_length_7262_cov_8.005273"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="PLZ74_08085"
     CDS             <1..128
                     /locus_tag="PLZ74_08085"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOR98320.1"
                     /translation="QGAGLVVPVTGDIKLMPGTASNAAFRRIDIDTATGKVKGLF"
     gene            412..1032
                     /locus_tag="PLZ74_08090"
     CDS             412..1032
                     /locus_tag="PLZ74_08090"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOR98321.1"
                     /translation="MSAKMPDATDSKEAKQSICDAMREKRRAVSQETRREAGRAICER
                     VIGPPLNLLMRTWRICIYLSTRNEIPTRYIAREIWNAGREVCVPAWSTSENGYRLYAI
                     DPAMRLISGHHGVREPEVRIPVMPWDVQVFIVPGLVFDTRGGRLGYGAGYYDRILAQA
                     PRSALKIAVCYDWQVIDTPLPQKPHDIPMDWIVTEQRALRCETEPT"
     gene            1029..1820
                     /locus_tag="PLZ74_08095"
     CDS             1029..1820
                     /locus_tag="PLZ74_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98322.1"
                     /translation="MNHIVASWLIMAVTAFTSFAQQGTSTSFSNVTDDTPKADPSQAR
                     KETTRLIVPDDFRWEIPIVSESNLPDLPLGRTNEFWIYTKAMFEAPPNSRLWFMLNIP
                     CAGFVKDKLNWAVNCYFVPTNISDRLCRGNAVHLNSRDHSPEQDHCQKGYSFDYGYVL
                     PPEENFKNHAPPIFMLPFRIHGNLDDQTIVQIADTVRELVQRPTSLLRELVRGPISSM
                     RLQEDGRVKVTTATSRAFLAGRGMEYILEKRDGAWIVVKTSHWVS"
     gene            complement(2048..2971)
                     /locus_tag="PLZ74_08100"
     CDS             complement(2048..2971)
                     /locus_tag="PLZ74_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOR98323.1"
                     /translation="MSIRSRGVSRRAFLSGAMSLGAATAIPGRAFGAAGRPDSKINGV
                     QIGTITYSYRSMPGKAEDVLRHVVASGINSIELMGNTAESFAGFPGGNAKDPVKARAM
                     LEWRIAAPMDKYEALRKLYQDAGVAIHIVKFGDIGNAGMSDEQIEYYFRVAKALGACG
                     ITREISEEAAKRLGPIADRHGIAIGFHNHMQINATTYDGPILSYGNTLKINLDIGHYT
                     AANEDSVLAVIEKYRTRLLSLHLKDRKKGKGPNMPFGQGDTPIVAALQYLKDTRLNIP
                     ADIELEYPVPQGSDAVKEVIRCVAFCKAALA"
     gene            complement(3187..5601)
                     /locus_tag="PLZ74_08105"
     CDS             complement(3187..5601)
                     /locus_tag="PLZ74_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98324.1"
                     /translation="MVKFETGRETVLPKEDSNDKPPVKRYYGPETPAKMLAHMKATGR
                     RTTWIENGVLYVDGKPCLRRNLYGVGYLCSKQHLERLTKTDDPGCTWEMTGVGIEFGR
                     LVPGSEEREGKKAQRPSQPVFDAVAKVIDEWKDKDFGYYYLCDEPECRGISPAYLRYI
                     YQFIKERDPYHVVNICSRDALRYIECTDMVEVHPYLAPCFNAEGKRVYGTELCTFGNY
                     VEGVVALNRADKLIGCTPGAFAYDKFGTSRDAPTFEEYVACVWAILIEGGKSIYPFIA
                     RALATRPCLYEGVRYTFSSAAALEDMLLFGARRRLAKTETHGAAYWEKGGERMFAVVN
                     FTAEPQAVELKGVKGSFGEFCGKRRWRTGRFGLSEGNIPLCLKPFETIIATTTPRDGG
                     LPTYAEVQALVKRLEDERVRRDNQLLGRVDDLSFAASKPLGMQTGLFNGMYDDLTEHF
                     RGDPWYEISFPKGAITFDRILLYGANLEKTTVKVRQKGVWVDLVPRSTERSESCIAFA
                     FDRPVRTVKVRFAFHKDKVELNEIEFPHLQQPVDKPVAAKVRVSAFQTATGSSRLFHA
                     ENLSFTNVWSGEKWYAKDIDVRRYDDGSFSLGPSGATHAIVFDPKWKWIDFEIPRPAV
                     KVTERGYSNWGLRLLKHPSGALCGNVRSCQTGLYTIPLVAIEKPLHGYVQVAAKSMHV
                     PFSYIRFSEAPPIRLSVLPTSTNGTIRVGDRLRVALTLAEPCEDVTLSFNVDRVTPCP
                     FGINGSSSMDLVCLDGEGRVWGGELEVKRLSDPHPRVGVYAKVDTLGGPRPLTVLTVI
                     PGKFIQ"
     gene            complement(5793..6992)
                     /locus_tag="PLZ74_08110"
     CDS             complement(5793..6992)
                     /locus_tag="PLZ74_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOR98325.1"
                     /translation="MKKRERLSKSMLFFVFFIVPLWLAPRSEAVEINDIGGRADGTGD
                     ISAVLQTAFAKGVRDVHFPKGEYCIGSMLLLPSGTKLLADKEARFVVKAGYAHEWVVS
                     NADHENGNADITIEGGLWDGNCDERPGKTFSPDEKHAGLFFHFNNVRRLTLKNFHAYD
                     SVTYHFSLGRVTDFEITNLRIGGERRPFCQDGIHMGGGCERGHVKGIVAHTGGMGDDL
                     IALNADDVFFYAWNRDQVALPIRDITVENVTATNCFTVIRLLSVHQPIERCTFRNFTA
                     SYRMHGLNLDAARYCAHPIFKDGEHPNGVGNLKDITFENFEIWGATAGKREVVTYETN
                     GDNVRFRNFRYRRDLDPNKERAVFLFRKMGPTEILREGEKKILANGETLKLSDTFYGD
                     LRISQAN"
BASE COUNT         1636 a         2182 c         1915 g         1529 t
ORIGIN      
        1 atcagggcgc ggggctggtg gtgccggtca cgggcgatat caaactcatg ccgggtacgg
       61 cctctaacgc ggctttccgc cgtatcgata ttgacaccgc caccggtaag gtcaagggat
      121 tgttttgagc ggcaatcgac tggtgacagg gatgtgcgaa gatcatgcgt ttctggtgaa
      181 aagaggagca gagagtcggg agcgcgggcg tcctcgcccg caacaacgtg tctcgcaaaa
      241 ggcggatgcg cccactttcc ttgggaacca aaccgcgcat cggaatctca atcggccttt
      301 cacagagcgg gcggggacgc ccgcgctccc gactgcgcac acacaaaaca cgcactcgac
      361 tcgcatgccc ctgtcactgt ccgattacgc gcaacgagaa agcaatgagc gatgagcgca
      421 aaaatgccgg atgcgacgga ttctaaagag gctaagcagt cgatctgcga cgcgatgcgc
      481 gaaaagcgcc gtgccgtgag ccaagagacg cggcgcgaag ccggacgcgc catttgcgag
      541 cgggtgatcg gcccgccact caatctgctg atgcgcacat ggcgcatctg catctatctc
      601 agcacgcgta acgaaatccc gacacgctat atcgcgcgag agatctggaa tgccggacgc
      661 gaggtgtgtg tgccggcttg gagcacctct gaaaacgggt atcggctgta cgcgatcgat
      721 cccgcgatgc gcctgatctc cggccatcac ggcgtccgcg agccggaggt gcgcattccc
      781 gtgatgccgt gggatgtgca ggtgtttatc gtgccggggc tggtgtttga cacgcgcggc
      841 gggcgcttag ggtatggtgc aggatactat gaccggattc tggcccaggc gccccgcagc
      901 gcgctcaaaa tcgcggtctg ttatgactgg caggtgatcg acacccccct gccccagaaa
      961 ccgcacgaca tccccatgga ctggatcgtg acggaacagc gggctctccg ctgcgaaacg
     1021 gagccaacat gaaccatatc gtcgccagct ggttgatcat ggctgtcacc gcattcacat
     1081 ctttcgcaca gcagggcacg tccacctcgt tctcgaatgt gacagacgac acaccaaagg
     1141 ctgacccaag ccaggctcgc aaagaaacaa cccgcctcat tgttccggac gattttcgat
     1201 gggaaatccc gattgtatcc gagtcgaatt tgcctgattt gcctctcggc aggaccaacg
     1261 aattttggat atacacaaag gcgatgtttg aggctccccc aaactcgcgt ctgtggttta
     1321 tgctcaacat accctgtgcg ggattcgtga aggacaagct taattgggcg gtgaactgtt
     1381 atttcgtgcc taccaatatc tccgacaggt tatgtagagg caacgccgta catctcaatt
     1441 caagagacca tagcccagaa caagatcact gccagaaagg gtattcgttc gattacggat
     1501 acgtcttgcc accggaggaa aacttcaaaa atcatgcccc gccaattttt atgcttccgt
     1561 tcaggatcca tgggaatctg gacgatcaga ctattgtcca gattgcagac accgtgcgcg
     1621 aacttgtcca acgaccgact tcattattac gcgaacttgt ccgaggaccg atttcatcaa
     1681 tgcgcctgca agaagatggg cgggtaaaag ttacgacagc aacttcgcgc gccttccttg
     1741 ccggacgagg catggaatac attctggaaa agagggacgg agcctggatt gtcgtaaaga
     1801 cctcgcattg ggtttcgtga ttccgatgac caaccctctt ttattttggt gtcatgaatt
     1861 gtgagacaag gagccgcgta cccgattcgc gcaaaacgat cacagggcgg gcgaggacgc
     1921 ccgcgctccc gactcaggaa ccgataccat tgccctgagg cgtcccacac accagtgaaa
     1981 cgcccatcct cctgtccact gcacaacaaa gtaatgcacg aattaactca tgcacgatcg
     2041 ttccttccta cgccagcgcc gccttgcaga acgcgacgca gcggatgacc tctttcaccg
     2101 catctgaacc ttgcggaacc ggatattcca actcgatgtc tgcgggaata ttcagccggg
     2161 tgtctttcag gtattgcagc gccgcaacga tcggtgtatc accctgaccg aacggcatgt
     2221 tggggccctt gccctttttg cgatccttga ggtgcaggct gagcagacgg gtacggtact
     2281 tttcgatcac ggccagcacc gaatcctcat tcgccgccgt atagtggcca atgtccagat
     2341 tgatcttcag cgtattgccg tacgagagga tcgggccgtc ataggttgtc gcattgatct
     2401 gcatatggtt atggaaaccg atcgcgatcc cgtggcggtc cgcgatcggc cccaaccgtt
     2461 tggcggcctc ttcggaaatc tcacgcgtga tgccgcaggc accgagggcc ttggccacgc
     2521 ggaaatagta ttcgatctgc tcgtccgaca tccctgcatt accgatgtcg ccaaacttca
     2581 cgatgtggat cgcgaccccc gcatcctgat acaacttgcg cagtgcctcg tacttatcca
     2641 tcggcgcggc gatccgccac tccaacatag ccctggcttt caccggatcc ttcgcgttac
     2701 cccccggaaa ccccgcgaag gactccgccg tgttacccat cagttcgatg gaattgatgc
     2761 cgctggcgac cacatgccgc aacacgtctt cggctttgcc cggcatcgac cggtaactgt
     2821 aggtgatcgt cccgatctgc accccgttga ttttcgaatc cggccttccc gccgcgccga
     2881 acgcgcgccc cggtatcgcc gttgccgcgc ccagcgacat cgccccgctc aaaaaggccc
     2941 tgcgtgaaac acccctgctc cgtatgctca tttcatctcc ctctcgttca atcctgaaac
     3001 tccggacaac gggctttccg catccgcggt ttccgcccta tcccgaacat tcgggtcatg
     3061 tgctgccagt ataggataaa gacgacccaa agggcaatcc ggattgagag cacccattct
     3121 caactcgacg tgcgcttgaa cacaaaacgt gtggctggac gacaagaaaa agaacgacca
     3181 tgacacctac tgaatgaatt tacctggaat caccgtgagc accgtcagcg gacgcggccc
     3241 accgagggtg tcgaccttgg catatacccc aacacgcgga tgcggatccg aaagacgttt
     3301 cacttcaagc tcgccacccc agacgcgtcc ctcgccatcc aagcagacga gatccatcga
     3361 agaggacccg ttgatgccaa acggacacgg tgtgacgcga tcgacgttga acgacaacgt
     3421 gacatcctca cagggttcag ccagggtcaa tgcgacacgc aagcggtcgc caacacggat
     3481 cgtgccgttg gtcgacgttg gcaggacaga cagccgtatc ggcggcgctt ccgaaaagcg
     3541 aatataggag aacggcacgt gcatcgactt ggcagcgact tgaacatacc cgtggagggg
     3601 tttttcgatc gcaacaagcg gaatcgtgta aagtcccgtc tggcaactgc gcacattgcc
     3661 gcaaagcgca cctgacggat gcttcagaag ccggagcccc cagttcgaat agccgcgctc
     3721 cgtgaccttg acagccggtc ggggaatctc aaagtcaatc catttccatt tgggatcaaa
     3781 cacgatcgca tgtgtggccc ccgaaggccc caaggaaaag ctgccatcat cgtagcgcct
     3841 cacatcgatg tctttcgcat accacttctc gccactccag acatttgtga acgagaggtt
     3901 ttcagcatga aaaagacgcg aagaccctgt tgcagtctgg aaagccgaaa cacggacttt
     3961 tgccgccacg ggtttatcaa ccggttgctg caaatgcgga aactcgattt cgttcaactc
     4021 taccttgtct ttgtgaaacg cgaagcggac cttcaccgtg cggaccggac gatcaaaggc
     4081 aaacgcgatg catgattcgc tccgttcggt cgaacgcgga acaaggtcaa cccagacacc
     4141 tttctgacgc accttcaccg ttgtcttttc gagattcgcc ccgtaaagca ggatgcggtc
     4201 aaatgtaatg gcacctttcg gaaaactgat ctcataccaa ggatcgccgc ggaagtgctc
     4261 ggtcagatcg tcatacatgc cgttgaaaag ccccgtctgc atgccgagcg gcttggatgc
     4321 cgcaaaggac aggtcgtcca cacggccaag cagctggtta tcgcgacgaa cgcgttcgtc
     4381 ttccaaccgc ttgacaagcg cctgcacttc cgcataggtg ggcaatcccc catcccgagg
     4441 agttgtagtc gcgatgatcg tttcaaacgg tttcaggcac aacggaatgt tcccctccga
     4501 aagtccgaac cgtcccgtgc gccacctgcg cttgccacag aattcgccga acgatccctt
     4561 cacccccttt agctcgaccg cttgaggctc ggccgtgaaa ttgacgacgg caaacatccg
     4621 ctcgcccccc ttttcccaat aggccgcgcc atgcgtctcg gtcttggcaa gccgacggcg
     4681 cgcaccgaaa aggagcatgt cttcaagcgc cgccgcggat gaaaatgtat agcgcacccc
     4741 ctcgtaaagg cagggtcgcg ttgcgagcgc tctggcgatg aacggataga tggatttccc
     4801 gccttcgatc agaatggccc agacacaggc aacatactct tcaaaagtcg gagcgtcccg
     4861 agatgtgccg aatttgtcat aggcaaaggc acccggtgta cagccgataa gcttatccgc
     4921 acgattcagc gccacaacac cctcgacata attgccaaac gtgcagagtt ccgtcccgta
     4981 aacgcgcttg ccctcggcat tgaagcaggg cgcgaggtac ggatgcacct cgaccatatc
     5041 cgtgcactca atataacgaa gcgcatcacg cgagcagata ttgacaacgt gatacggatc
     5101 cctctcctta atgaactggt agatgtagcg gagataagcg ggcgagatac cgcggcattc
     5161 tggctcatca caaaggtaat agtaaccgaa gtccttgtcc ttccactcgt caatcacctt
     5221 cgcgacagca tcgaagacgg gctgcgatgg acgctgggcc ttcttgcctt cgcgttcctc
     5281 gctacccggc acgagccgcc cgaattcgat tccgacgcca gtcatttccc aagtgcaacc
     5341 gggatcgtcc gtcttcgtca ggcgttcaag gtgctgtttc gaacacaggt agccgacacc
     5401 atacagattg cgccgcagac acggctttcc atccacgtag aggacgccgt tctcgatcca
     5461 tgtcgtgcga cgccccgtcg ccttcatgtg cgccagcatc tttgcaggcg tctcggggcc
     5521 gtaataacgc ttcacaggcg gtttgtcgtt cgagtcctcc ttcggcagga cagtctcgcg
     5581 ccccgtttca aacttcacca tcttacgcgt ctcttcatcg acgttccacg ccttcttctg
     5641 cgcagatccg gcaactgacg caccgaccca gaaaatgacc aagggcaaga gaagttttct
     5701 gtttatatgc actttcacct cacatacggc acttcccgcc agccagcagc cagcgcaacg
     5761 ctggccgctg gctggcgggc ctgtaaaaat gatcaattcg cctggcttat ccgcagatcg
     5821 ccgtagaacg tatcggataa tttcaacgtc tcgccgtttg ccagaatttt tttctcgccc
     5881 tcgcgcaaaa tctcggtcgg tcccatcttg cggaaaagaa agacggcgcg ttccttgttc
     5941 ggatccaaat cgcggcgata acggaagttg cggaagcgaa cattgtcacc gtttgtctcg
     6001 taggtgacga cctcgcgctt gccagcagtc gctccccaga tttcaaagtt ttcgaaggtg
     6061 atgtccttca ggttccccac gccattcgga tgttcaccgt ccttgaagat cgggtgcgcg
     6121 caatagcgcg cggcgtcgag attaaggccg tgcatacggt agctggccgt gaagtttcgg
     6181 aacgtgcagc gctcaatcgg ctggtgcacc gacaggagac ggatcaccgt gaagcagttc
     6241 gttgccgtca cgttttcgac cgtgatgtcg cgaatcggca gcgcgacctg gtcgcggttc
     6301 cacgcgtaga agaagacgtc gtccgcattg agtgcgatga ggtcgtcgcc catgccgccc
     6361 gtgtgcgcga caatgccctt gacgtgtccg cgctcgcaac cgccacccat gtggatcccg
     6421 tcctggcaaa acggacgcct ctcgccgcca atcctaaggt tcgtgatctc gaagtccgtg
     6481 acgcggccaa gcgaaaaatg gtaggtgaca gaatcatagg cgtggaagtt ctttagcgtc
     6541 agacgtcgca cgttgttgaa gtggaagaaa agcccggcat gtttctcgtc cggcgaaaag
     6601 gtcttcccgg gacgttcgtc acagttgccg tcccacagac cgccttcgat cgtgatgtcg
     6661 gcattgccat tctcatggtc ggcattggac accacccatt cgtgcgcata cccggccttg
     6721 acgacgaaac gagcttcctt gtcggcgagc agttttgtgc ctgacggcaa caacagcatc
     6781 gaaccgatgc agtattcgcc ctttgggaag tgaacatcac gcacgccctt tgcaaaggcc
     6841 gtctgcaaaa ctgccgaaat gtcgcccgtt ccgtccgccc ggcctccgat gtcgttgatt
     6901 tctaccgcct ccgaacgagg ggcaagccat aatggcacaa taaaaaacac gaagaacaac
     6961 atgctcttgc ttaatctttc acgtttcttc attctttttt cccttttctc ttttcttgca
     7021 ttgtcatttt ctcgtttaga atctcaacaa cctccctcag ttaagacgat tatagcaaac
     7081 cattgtcacg cctcaagtag atcgagtaaa tgaatactgt ggcagcggat gctgaccgtt
     7141 atcgagggcg tttcgcgtga ccgtacgtaa aacaaaaaag ccaataaaac cgaggcttta
     7201 ttggtcttca agatggtggc gggtgagggt cacgatcccc cgacctgcgg attatgattc
     7261 cg
//
LOCUS       DAOSRB010000144         7244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9597_length_7244_cov_5.954514, whole genome shotgun sequence.
ACCESSION   DAOSRB010000144 DAOSRB010000000
VERSION     DAOSRB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9597_length_7244_cov_5.954514"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1021)
                     /locus_tag="PLZ74_08115"
     CDS             complement(14..1021)
                     /locus_tag="PLZ74_08115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR98326.1"
                     /translation="MRRLSILIVTYDGDELLRACLDSIQRACGDMPGTVVVDNAASET
                     TRELVAAFPRTRYVRAETNLGFAGGNNLGFPFCEGEYILLLNNDTVIHDEPFTALIRY
                     CDANPQVGVAQGKIRFGGNIDLLDGCGSFLTAIGIQYAYGFLVPDAPAFQKPYPVFAA
                     SGAFFLIRRSVIAQAGGYLFYDHFRSYYEEVDFCHRVWLSGSEVHFVPTPVIDHLHSQ
                     TAGRFRRTDILRQYYQNIWFSLLTCLGGYGRLRILPVFTAFFAGNALAHLLAGHRELF
                     RAHLDTVRKLWIMRDQIRSARKTIRGFRVRSDRELFRIILKSPPLSYYWRSIRSNAFS
                     S"
     gene            1193..3304
                     /locus_tag="PLZ74_08120"
     CDS             1193..3304
                     /locus_tag="PLZ74_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98327.1"
                     /translation="MNGKKVFAATVMMAGMALQGLAMLRCETQPDLTVHVRPGGDDAG
                     PGTQAHPLGTLAKARDMVRTARQQRALTTGAEIVLHGGRYVLNDTLMLSAEDGGTSAA
                     PIRYRAARGEQPVLAGSTEIPASAFLPLADPERLARLEAPARERVVAVDLKALGFPAM
                     PKPRDVFRMPMCVPELFCNGRRMTLACWPNEGFAHIVKILDMGTQRGTGSVSDSADPK
                     KKRTQENRGGCFVYEGDRPARWRVEEGVWLHGFWCFDWYDDVIRIGAIDAEKKEIRLQ
                     AAHEYGVRQGNPSPRRWRAVNVFDELDAPGEYMVDLQAGRLYFYPPEPLAGSRITLAF
                     RGEPLVRMRDLSHVIFEGLTFEETQGDALRVTGCSHVVVSRCVFRNHRMRAIVVEGGV
                     KNRIASCDIHDTGVGGVILDGGDRKTLSPAGHVLEDTHIWRYSQHLQTYASALTLKGV
                     GNVARHNLLHDAPHMAVAISGNDHLFEYNVVSNVVMASDDAGALYKGRNPSCRGNVIR
                     YNLWSEIGSPRGHGTAAIYFDDGDGGDTVYGNLFYRCGDPGKGSFGTVFSHGGHGNVA
                     ENNVFIECKRALGSSPWNDKRWKQFIDAPLWQERLLREVDITSGTYTGRYPELIGFMD
                     PQPGALRENIAIRNLFVACDGITKGNWNTNAVYVATAEDPGFVNYAKRDFRLKRNAAV
                     WKRMPEFKPLPLEKMGLRRKR"
     gene            complement(3343..6879)
                     /gene="dnaE"
                     /locus_tag="PLZ74_08125"
     CDS             complement(3343..6879)
                     /gene="dnaE"
                     /locus_tag="PLZ74_08125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOR98328.1"
                     /translation="MNKPFVHLHLHTKYSLLDGACHIEPLVERAKELGMNALAMTDHG
                     VMYGAIDFYNACRDAGIKPILGCEIYILAKAHRGTRDHTIPYHHLVLLARNREGYINL
                     ARINTISHLEGFYYKPRIDKETLRAHAGGLIGLSACLKGEVNALLAERHLDEAEKVAL
                     EYRDIFGPDNFFLEMQDHGLPEQKRVNEGVRELCRRTGLKAVVTNDVHYLHQNHAEAH
                     EVMLCIQTQTVMSDPKRMRYASDEFYFKSREEMEKRFPDDREAFDLTQEIADRCDVQL
                     TFSNGKAETLHFPAFDVPAGFEDARQYLVHLGKQGLKRHYGFEYDDPRDATQKMLVER
                     FDYEVGVIENVGFINYFLVVADFVNWSRNNGVPVGPGRGSGAGSLLAYSLGITQIDPI
                     RFDLIFERFLNPERVSPPDFDIDFCQMRRERTIEYVKRKYGKERVAQIVTFGQLGAKT
                     VIRDIARVLEIPLYQADKFSKMIPEDPKMTLAKAKTENPQFAEACAQDPLLQQIMRHA
                     VVLEGLYRNAGVHAAGVVIGDKPLIDLIPLGRDKDGSPVTQYAKEPVEACGLLKMDFL
                     GLKTLTVLQESVELVRMTRGIEVDLENLPLDDPKTFDLFRRADTVGVFQLESDGMRRT
                     LVDLQPTRIEEIIAILALYRPGPMDMISLFVKRKKGEEKITYDHPLLEPILKETYGIM
                     VYQEQVQRAAQVLAGYSLGKADMLRRAMGKKKAGEMAKERTGFIAGCGKTNGIPEEQA
                     GQIFDNIEKFAGYGFNKAHAAAYGMITYQTAYMKAHYPVEFMCAQISSEIGNFDKLPG
                     FVAEAEAMGIRVLPPDVNHSLDRFAPENGNIRYGLAGIKSVGTGAAEAIIQARAQTAF
                     TGLTDFCERVDPSSVNKRVLEALIRCGAMDAFGMHRARLFFNIDFALARANEKLREKA
                     SGQRSLFEMLDADEANAVCTDDLPDYPAWSEKERLTGERELLGMYMTGHPLTRYRRII
                     QALQTFSAARGEETPDKQDVRIAGMAASVVTRISKKTKEPWAIITLDDGETNIEVLVY
                     TRAFRKYEGACRQDQPVLVCGKLSKKEDQIKIIAEEVYPLIEAPRQFVEKVVVGIRTD
                     DGQTSGRIKALRSLVDRHPGITPILICLLYPDSKRMLIRTNEAMTIDPSPEFIAEAEG
                     ILGPDHFRFTARKAIYREAHDDSRWGWNGTDR"
BASE COUNT         1432 a         1964 c         2357 g         1491 t
ORIGIN      
        1 ggcggtccgg ggatcaggat gagaaggcgt tggatcggat ggaacgccag taatagctca
       61 atggggggga cttgaggatg atccggaaca gttcgcggtc ggaacgtacg cggaacccgc
      121 ggattgtttt gcgcgccgag cggatctgat cccgcatgat ccagagtttg cgcacggtat
      181 ccagatgggc gcggaacagt tcgcggtgac cggccagcag atgcgccagc gcgtttcccg
      241 caaagaaagc ggtgaaaacc gggagaatcc ggaggcgtcc gtatccgccc aggcaggtca
      301 gcagcgaaaa ccaaatgttc tggtaatact ggcgcaggat atcggttctc cggaaacgcc
      361 cggctgtctg gctgtgcaga tggtcgatga caggcgtggg aacgaaatgc acctcggatc
      421 cgctcagcca gacgcggtga cagaaatcga cctcctcata atacgaacgg aaatgatcgt
      481 aaaaaagata tccgcctgct tgggcgatca cagagcggcg gatcaggaaa aaggcaccgc
      541 tggcggcgaa gaccgggtac ggtttttgga aggcgggcgc gtcagggacc agaaacccgt
      601 aagcgtactg gatgccgatt gccgtcagaa aggagccgca tccgtccaac aggtcaatgt
      661 tgccgccgaa tcgtatcttg ccttgcgcaa cgccgacctg cggattcgcg tcacagtacc
      721 ggattagcgc ggtgaacggt tcgtcatgaa tgaccgtgtc gttgttcagc aacaggatgt
      781 attcaccctc gcaaaacgga aaccccagat tgttgccgcc tgcgaaaccg agattggttt
      841 cggctcggac gtagcgcgta cgggggaagg ccgcaaccag ttcgcgggtc gtctcactgg
      901 cggcgttgtc gacaaccacg gttcccggca tgtcgccaca ggcccgttga atggagtcca
      961 gacacgcccg cagcaattcg tctccgtcgt aggtgacgat caggatggat aaacgacgca
     1021 aagcgtccga ggggtgcggc aacaacatgc ccggaatata atcgaaacgg ggagaggaaa
     1081 gcaagcgcgg acaaaagagg cggaggcggg aaagggcttg gggttgcgtt cctctgatgg
     1141 agatgtcata ctgcaaacgg gatgcacgaa aggaaaacaa aggagaaata cggtgaacgg
     1201 gaaaaaggtg tttgcggcaa cggtgatgat ggccgggatg gcgttgcagg ggttggcgat
     1261 gctgcggtgc gagacccaac ccgatttgac ggtgcatgtg cgtcccggcg gtgatgatgc
     1321 tggtccaggg acacaggcgc atccgctggg caccctcgcc aaggcgcggg atatggtgcg
     1381 gaccgcccgt cagcaacgtg cgctgaccac tggtgccgag attgttttgc acggtggccg
     1441 ttatgtgtta aacgacacgt tgatgctgtc ggcggaggat ggcggcacat ccgcagcacc
     1501 aatccgatac cgtgcggccc gcggcgagca gccggttctg gccggatcga cagagattcc
     1561 ggcgagcgct ttcctccctc tcgcagatcc ggagcggctc gcgcggctgg aagcacccgc
     1621 ccgcgaaagg gtcgtggccg tggatctgaa ggcgctaggg tttcccgcga tgccgaaacc
     1681 ccgcgatgtg ttccggatgc cgatgtgtgt gccggagctc ttctgcaacg gtcggcggat
     1741 gacgctggcc tgttggccga atgagggctt cgcgcatatc gttaaaattc tcgacatggg
     1801 gacgcagcgc gggaccggtt cggtgtcgga ttcggctgac cccaagaaaa agaggacgca
     1861 ggaaaatcgc ggcggatgct ttgtctatga gggggaccgg ccggcgcgct ggagggtgga
     1921 ggagggggta tggctgcacg gcttctggtg ttttgactgg tatgacgacg tgatccggat
     1981 tggcgccatc gatgcggaga aaaaggagat tcggttgcag gccgcgcatg agtacggggt
     2041 gcggcagggt aacccctcgc cgcgtcgctg gcgtgcggtg aatgtgtttg acgagttgga
     2101 cgcgccgggc gaatacatgg tggatttgca ggcggggcgg ctctatttct atccgccgga
     2161 accgcttgcg ggttcccgga tcacgctcgc gtttcgcggg gagccactgg tacggatgcg
     2221 ggatctttcg catgtgatct tcgaggggct gaccttcgaa gagacgcagg gtgatgcgtt
     2281 gcgtgtgacc ggctgtagtc atgtggtcgt cagtcggtgt gtgttccgca atcaccgcat
     2341 gcgggcgatc gtggtggagg ggggtgtgaa aaaccggatc gcctcctgtg acattcacga
     2401 caccggtgtc ggcggggtga ttttggatgg gggggatcgg aaaacgcttt cacctgcggg
     2461 acatgtgttg gaggacaccc acatctggcg gtattcccag cacctccaga cctacgcttc
     2521 cgccttgact ctgaaagggg tggggaacgt ggcgcggcac aacctgcttc atgatgcgcc
     2581 gcatatggct gtcgcgatca gcgggaacga ccacctcttc gagtacaacg tggtgagcaa
     2641 tgtggtcatg gcctctgatg atgcgggcgc actgtacaag gggcgcaacc cttcttgccg
     2701 cggtaatgtg atccgctaca atctgtggag cgagatcggc agtccgcgcg gtcacggcac
     2761 ggcggcgatc tactttgatg acggggatgg cggggacacc gtttatggaa acctgtttta
     2821 ccgttgcggc gatcccggca aagggtcgtt cggcacggtc ttctcccacg ggggacacgg
     2881 gaatgtggcc gagaacaacg tgttcatcga gtgcaagcgc gcgctgggct cgtcaccgtg
     2941 gaacgacaag cgttggaaac agttcatcga cgcaccgcta tggcaggaac gtctgctgcg
     3001 ggaggtggac atcaccagcg gaacctatac cggacgttat ccggaactga tcgggttcat
     3061 ggatccgcaa cccggtgcgc tgcgcgagaa tatcgcgatc cggaatctgt ttgtggcctg
     3121 cgacggcatc acgaagggca attggaacac caacgccgta tatgtcgcga cggcggaaga
     3181 tccgggattc gtcaactatg ccaagcgcga tttccgcctg aaacgtaacg ccgcggtttg
     3241 gaaacgcatg ccggagttca agccattgcc gttggaaaag atgggcttgc ggcgtaagcg
     3301 gtaaaggcgc agtccggctg tgccggatcc caatcgaaac ccttatctgt cggtgccgtt
     3361 ccagccccag cgggaatcgt catgcgcttc ccggtaaatg gctttgcgag cggtgaaacg
     3421 gaagtgatcc gggccgagga tgccctccgc ctcggcgatg aactcgggtg aagggtcaat
     3481 ggtcatcgct tcattggtgc ggatcagcat gcgtttgctg tccggataga ggaggcagat
     3541 caggatgggg gtgatgccgg gatgacggtc caccaaactg cgcagcgcct tgatccgtcc
     3601 cgaagtctgg ccgtcatccg tacggatgcc gaccaccact ttctccacaa actggcgcgg
     3661 cgcctcgatc aacgggtaaa cctcttcggc gatgattttg atctgatcct cttttttgga
     3721 tagtttgccg cagacgagca ccggctgatc ctgccggcac gctccctcat atttccggaa
     3781 cgcgcgcgta tacaccagca cctcgatgtt cgtctcgcca tcatcaagcg tgatgatggc
     3841 ccaaggctct ttggtttttt tgctgatccg ggtgacgacg gaggcggcca tgccagcgat
     3901 gcggacatcc tgtttgtccg gggtctcctc gccgcgggct gccgaaaacg tctggagcgc
     3961 ctgaatgatg cggcggtacc gggtgagggg gtgtccggtc atgtacatgc cgagcagctc
     4021 gcgttctccg gtgaggcgtt ccttttccga ccaggccggg taatcgggca ggtcatcggt
     4081 gcagacggcg ttggcctcgt cggcatccag catttcaaag aggcttctct ggccactggc
     4141 cttctcgcgc agcttttcat tcgcgcgggc caaagcgaaa tcgatgttga aaaaaaggcg
     4201 ggcgcggtgc atgccgaacg cgtccatggc accgcaacgg atcagggctt ccagcacgcg
     4261 tttgttgacc gaggagggat cgacgcgctc acaaaaatcg gtcagcccgg tgaatgcggt
     4321 ttgcgcccgg gcctgaataa tcgcctctgc ggcaccggtg ccaacgcttt tgatgccggc
     4381 gagcccgtag cggatattcc cgttttcggg cgcgaaccgg tccaaactgt gattcacatc
     4441 cggcggcagc acgcggatgc ccatcgcctc ggcttcggcg acaaatccgg gcagtttatc
     4501 gaagttgccg atttccgagg agatctgggc gcacatgaac tcgaccggat aatgcgcttt
     4561 catgtaggca gtctgatacg tgatcatgcc gtacgcggcg gcgtgggctt tgttaaaccc
     4621 gtatcccgcg aacttctcga tgttgtcgaa gatctggccg gcctgctcct cggggatgcc
     4681 gttcgtcttt ccgcatccgg cgatgaatcc cgtgcgttcc ttggccatct ccccggcttt
     4741 tttcttgccc atggcccggc gcagcatatc ggctttgccg agcgagtatc cggccagaac
     4801 ctgagccgcg cgttgcactt gttcctgata gaccatgatg ccataggttt ctttcaggat
     4861 cggctccagc agaggatggt cgtacgtgat cttctcctca ccctttttgc gcttcacgaa
     4921 cagggagatc atgtccatgg ggcccggacg gtagagcgcg aggatggcga tgatttcctc
     4981 gatgcgggtg ggctgcaggt ccacgagggt gcgccgcatg ccgtcggatt caagctggaa
     5041 cacgccgacg gtgtcggcgc gccggaaaag gtcgaaagtt ttcggatcgt ccagcggcag
     5101 gttttcgaga tccacttcga tgccccgcgt catgcgtacc agttccacgg attcttgcag
     5161 cacggtcagg gtcttgaggc caagaaagtc cattttcaac agaccgcacg cctcgaccgg
     5221 ctctttcgca tactgcgtga cgggtgaacc gtccttatcc cgcccgagcg gaatcagatc
     5281 gatcagcggt ttgtcgccga tcaccacgcc ggccgcatgc acccccgcgt tacggtacag
     5341 gccttcgagc accacggcgt gccgcatgat ctgttgcagc agcgggtcct gggcgcatgc
     5401 ctcggcgaat tgcggatttt cggtcttggc tttggccaac gtcattttcg ggtcttcggg
     5461 aatcatcttg ctgaatttgt ccgcctgata aagcgggatt tccagcacgc gcgcaatgtc
     5521 gcggatcacg gttttggcac caagctgacc gaaggtgacg atctgcgcga cacgctcctt
     5581 gccgtatttc cgtttgacgt attcgattgt ccgttcccgg cgcatttgac agaaatcgat
     5641 atcgaaatcg ggcggcgaca cgcgctctgg attgaggaaa cgctcgaaaa tgaggtcgaa
     5701 gcggatcgga tcgatctggg tgatgccgag cgaataggcg agcagcgacc cggcgcccga
     5761 accgcggccg gggccgacag gaaccccgtt gttgcgcgac cagttgacga agtccgccac
     5821 cacgaggaag tagttgatga agcccacgtt ttcgatcacg ccgacctcat agtcaaaccg
     5881 ctcgaccagc atcttctggg tcgcgtcgcg cgggtcgtcg tattcgaacc cgtaatgacg
     5941 ctttaacccc tgcttgccga gatggaccag atactgccgg gcgtcttcga atccggctgg
     6001 cacgtcgaac gcgggaaagt gcagggtctc ggccttgccg ttggaaaacg tcaactgcac
     6061 gtcacagcgg tccgcgatct cctgggtgag gtcaaaagcc tcgcggtcat cggggaagcg
     6121 cttctccatc tcttcccgcg acttgaaata aaactcgtct gaggcgtacc gcatccgttt
     6181 cgggtcgctc atcacggttt gggtctggat gcagagcatt acttcgtgcg cttcagcatg
     6241 gttctgatgg aggtagtgga catcattggt caccaccgct ttcaggccgg tgcgccggca
     6301 gagttcgcgc acgccttcgt tgacccgctt ctgttccggc agcccgtggt cctgcatctc
     6361 aaggaaaaaa ttgtccggcc cgaagatgtc ccggtactcg agcgcgactt tttccgcctc
     6421 atcgagatgc cgttccgcaa ggagagcatt gacttcgcct ttgagacatg cggagagacc
     6481 gatcagacca ccggcgtgtg cgcggagggt ttctttgtcg atgcgcggtt tgtagtaaaa
     6541 gccttcgagg tgggagatgg tgttgatgcg cgccagattg atgtacccct ccctgttacg
     6601 cgccaacagg accagatggt ggtatgggat cgtgtgatcg cgcgtgccgc ggtgggcctt
     6661 ggccagaatg tagatttcac atccaaggat cggtttaatc cccgcgtcgc ggcaggcatt
     6721 gtaaaaatcg atggcgccgt acatgactcc gtggtcggtc atggccaacg cgttcatgcc
     6781 caattctttg gcacgttcga ccagcggttc gatgtggcag gctccgtcca gcaggctata
     6841 tttggtgtgg agatggaggt ggacaaaagg cttgttcata tgaggggctc cgccggccgg
     6901 cgcggggaaa gtgggaaacg gaaagggggc gcgccggaac cccggatccc ctttgctgtt
     6961 caggatagca aaacgctggg aaggcgggaa ggctgaaaaa cttttcgttc agggcgcgcg
     7021 ttgaggcggg caaaggaaga gacaagccag ggtttttcga cagcttcgct ttggcgggga
     7081 ggctgtgagg gtgttaggct gtgagggtgt gagggtgtga ggcggtgagg gtgtgagggt
     7141 gtgaggctgt atcgatcccg atagcgatag cgatcccgac cccgatttag atgaaaccat
     7201 gaaacacatg aaacttcatg aaaatcagga attccccctg gcgt
//
LOCUS       DAOSRB010000145         7122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9812_length_7122_cov_6.707938, whole genome shotgun sequence.
ACCESSION   DAOSRB010000145 DAOSRB010000000
VERSION     DAOSRB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9812_length_7122_cov_6.707938"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1275)
                     /locus_tag="PLZ74_08130"
     CDS             complement(115..1275)
                     /locus_tag="PLZ74_08130"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOR98329.1"
                     /translation="MIRVASCFAQVLSMIDRYGFDRAVRELDAERGAKGFRCRDQFVA
                     MLFCQMGSANSLREICGGLSTALGKMVHLGLKEAPKRSTLAYANSHRPWQLYRGVFEQ
                     LLERCRVLAAERRRPFRFKNPLRVIDSTVIDLCQEVFDWARYRRAKGAIKLHLQLQDR
                     GCLPCWALVTEGRTHDVRVAQGLSFDPGTIVVMDRAYTDYGMFGRWDAEGVFFVTRMK
                     ADARHEVVHSLPGGGGAFRGEEWIRLTGRAAEGCGCVLRRIRIWDAQNEREIVLLTNQ
                     RTLAAATIGRVYRYRWQIELFFKAIKRNLKIKTFVGTSENAVKTQIWTALIAILLLKF
                     MQMKSSWQWSLSNLAAMLRFNLLTYRDLWEWLDDPFGVPIREPDTIQLTLAL"
     gene            1881..3797
                     /locus_tag="PLZ74_08135"
     CDS             1881..3797
                     /locus_tag="PLZ74_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOR98330.1"
                     /translation="MEKAGRDGARARSWSVWLVTVGIGLAAGCALLDSGPSVPGGAPA
                     QTVDFGTPEGVQVSSDGLREAVVTWAPPTVKAYRYRIERSPAADGPFVMVAEVPPKAL
                     SYADGADPSSRLEDATAYHYRVIAVLERNGPVSPPSAVVRSVTAPPPVPPEAIRAEAT
                     GSRAVTVTWQASGSIGVTGYRVERAPVDEPTAFAPVSGEVSTLTFTDGGKPSSTLKDS
                     SEYLYRVLTVNRAGSSSAPSATARVKTLPRPAQVKGLKAVSREVRCVPLKWTPSPEAD
                     VTHYTVFRARSSEGPFEQIGTAQGRAGAEYTDGGANPGNLEDEGTYFYFVRAVNVVTA
                     ESIDSDTVRAVTREVPPEVKQVAAVSARPREVPVEWEASPDTAVVGYEVWRTLAGADD
                     WTQVGRVNSRETTRFLDRGGEQDATRLGKLQDGTEYQYRVVAFNTATVRSSASVPAPA
                     KTKVIPVPPSGLVATTNLPHAIRLTWSPNPEPDVDGYRLEASKKPDGGFRRWVEFRLN
                     EKTDLSAEEKELDPSQVRYYRVMALDREGLESLWSEPIEGRAKPLPDPPFDLQAETEG
                     ALRRITWQPPPQPDVVRYVVWEKWFLGWKRLADTDKPEFTLTLPPDQKPPTLAVSAVD
                     QDGLESRRSEAVNP"
     gene            3818..4939
                     /locus_tag="PLZ74_08140"
     CDS             3818..4939
                     /locus_tag="PLZ74_08140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOR98331.1"
                     /translation="MDTPFSYLESAAVTDVGRRRKNNEDALVELPACGVFCVADGMGG
                     AKGGEIASQATVEALQTVFEHSTDAPFAVTAAAAARLAARALNQASAWIRENAEERGL
                     TGCGSTAVMLLFDRINPACARVLHAGDSRAYRLRGGRLEQLSQDHSVAAAAGLKDEKD
                     LPAMFRGVITRAVGLEKSVRLEETLVEVHGADLYLLCSDGLSKMVSDRHIQKLLRKHA
                     TEPIGTLAQRLIDEALHAGGEDNISVILVRVADKLPPATVTELSPETLALEALDLDGV
                     PAAADSEDSEPLTRDTRETADTAGCECAKTPRDIDTDQAIQTPPTPFSGTGITPEENV
                     EKEEVAPKPRRRLLLRILVVCLMAGLAAAVWILFRTFRP"
     gene            4967..>7122
                     /locus_tag="PLZ74_08145"
     CDS             4967..>7122
                     /locus_tag="PLZ74_08145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOR98332.1"
                     /translation="MDETLKERIGDYELLELIGDGAQGRVYKARHMAGPEGEPQELVA
                     LKVVRITGEDEKLRLRFQEQAEILRRLNHAHIVAYRDCFTWHAGEWDEAQCLVMELLE
                     GETLAELLKHASSGLPWPQVEQIFEQCLAGLIHARECGITHRDIKPSNIFLTKGGQAK
                     IIDFDIARRDDSGQMSTAGWKGTFDYMAPDFISIDGFRGDETSDVFSLGICFYQALTG
                     SLPYGKLGEAAHIGYLNRWRDQAAPVPSFRPGVFRVLAHAKTVVSKAIAARREQRYAT
                     FAAFLEEFRMIRYRRIRNKNKDEYELLAVLGRGGFGEVFKARRVSDGLVVAVKFLFSE
                     KQSDRFLKEAKILQQYPHPNLVQYVDFIVIKGVTGEKQFFLVMEFLEGMPGWTLRVRL
                     KNEGKLEVEEAVTLFNCYLSALQFLHGSARPIIHRDIKPGNLYAPVGQPEKGKVFDLG
                     VARDVSGTVTVGGVPGTLDYMAPEFADAGGDRGSPQSDLYALGLCLYEVLAGKPVFER
                     LPGDLNSAWVAFQDRLRKPPEIAFDAEVFVRYPRFKGVILRALALKPDDRFPSADAMR
                     KALQEALLSGSAPQADEGFDADEGLTMATFVGARPAEPEPGATTGTRAVSSEVLQEAL
                     DEQARQADRRPRERAGRRGPGYLIAAIAGTAVTMTLLFGLVVYWLGREKPKAGQTARV
                     EKPATPTASAPAETKPAPTPEVAAAKPAAVAQPAEPS"
BASE COUNT         1273 a         2148 c         2361 g         1340 t
ORIGIN      
        1 cactgctgtc caaaacagtg ttcattgtac gggctgatct gttgatttac tagcgttttc
       61 aggttgttag acgggttgtt gggtttgagg tttcggaaaa tgctgtccaa ggtttcacag
      121 tgcgagagtc aactgtatgg tgtcgggctc ccggattggg accccgaagg ggtcgtcgag
      181 ccattcccac aagtcgcggt aggtcagcag gttaaagcgc agcatggccg ccaggttgga
      241 gaggctccac tgccacgacg acttcatctg catgaacttc aggagcagga tcgcgatcag
      301 tgctgtccaa atctgcgtct tgaccgcgtt ctcgctggtg cccacgaagg tcttgatctt
      361 gaggttccgc ttgatcgcct tgaaaaacaa ctcgatctgc cagcggtagc ggtagacccg
      421 cccgatggtg gccgcggcga gcgtgcgctg gttggtgagc aggacgatct cccgttcgtt
      481 ctgcgcgtcc cagatgcgga tgcggcgcag gacgcatccg cagccctcgg ccgcgcgccc
      541 cgtcaggcgg atccactcct cgccgcggaa cgcgccgccg ccgccgggga ggctgtggac
      601 cacctcgtgc cgggcgtcgg ccttcatgcg tgtgacgaag aagacgccct cggcgtccca
      661 tcgcccgaac atcccgtagt cggtgtaggc ccggtccatc accacgatgg tgcccggatc
      721 gaacgagagg ccctgcgcca cgcgcacgtc gtgcgtccgg ccctccgtca ccagcgccca
      781 gcagggcagg cagccccggt cctgaagctg caggtgcagc ttgatcgcgc ccttggcccg
      841 gcggtacctc gcccagtcga agacctcctg gcaaaggtcg atcaccgtgg agtcgatcac
      901 gcgcagcggg ttcttgaacc ggaagggacg ccgccgctcg gccgccaaaa cccggcacct
      961 ctcgagcaac tgctcgaaaa cgccccggta gagttgccac ggcctgtgcg agttcgcata
     1021 ggccagcgtc gagcgcttcg gcgcttcctt cagccccaga tgcaccattt tgcccagcgc
     1081 cgtgctcagc ccgccgcaga tctcccgcag cgagttcgcc gaacccatct ggcagaacag
     1141 catcgccacg aactggtccc ggcagcggaa acccttcgcc ccgcgctccg cgtccagttc
     1201 cctcaccgca cggtcgaacc cgtaccggtc aatcatcgaa agcacttgcg caaagcagct
     1261 cgccacgcgt atcataatct cgcctccgtt gtcgtgtccg cggcatcagc cgcggggatt
     1321 agttttttga tacatccatc gtacacgttc agcggaggct tatcacttca aaacgctgtt
     1381 ttggacaaga gtgtgtatta gtgtattagt gcattagtgc attagcctaa aaagagcagt
     1441 tgaaccttca actctctttg gtttcagggt ttcatccaaa tcggggtcgg ggtcgggatc
     1501 gctatcggaa tcggtatcgg gatcggaatc gctatcggaa tcggaatcgg gaacgtcgac
     1561 ccgcgacccg cgacattcga cccgcgacga tcgacaggga cgcccgcgtt tccgacaccg
     1621 caggtcgctc gtcgcgggtc gcgagtcgac ataaggtctg acgggagaag cgggcgtccc
     1681 gcatacgcac cggtctccaa aactcagtcg attgcgcttg cgacgaagtg gagcggcgaa
     1741 gcgtcgggac taagggttca aaaagagcgt cgggctcccc ccggcttttt tctccctttt
     1801 ctgtgaatcg gactggattt taccctgtcg atggagtatt ctttccgaca aatgccatga
     1861 ttcaggtgtg gaggccggat atggaaaagg caggacgcga cggagcgagg gcacggtcgt
     1921 ggagcgtgtg gctggtcacg gttgggatcg gcttggcggc agggtgcgcc ttgttggatt
     1981 cggggccgag tgtgccgggt ggcgcgccgg cgcagaccgt ggatttcggc acacccgagg
     2041 gggtgcaggt ttcgtcggac gggttgcgcg aggcggtggt gacgtgggcg ccgccgacgg
     2101 tcaaggcgta ccggtaccgg atcgagcgtt cgcctgcagc cgacggtccg ttcgtcatgg
     2161 ttgccgaggt gcctcccaaa gcgttatcgt atgcggacgg ggctgatcca tcctcgcggc
     2221 tagaggatgc cacggcctat cattaccgcg tgattgcagt gttggagcga aacggtccgg
     2281 tcagcccgcc ttcggctgtg gtgcggagtg tcaccgcgcc gccgccggtc ccgccggaag
     2341 cgattcgggc cgaggcgacc ggttcccgcg ccgtgacggt gacgtggcag gcttcgggtt
     2401 cgatcggggt gaccggatac cgcgtggagc gcgcgccggt tgatgagccc acggcgttcg
     2461 cgccggtgtc aggcgaggtc agtacgctca cgttcacgga tggcggcaag cccagttcga
     2521 cgctcaagga cagtagcgaa tatctgtacc gtgttcttac cgtcaaccgg gccggatcat
     2581 cgagcgcgcc gtccgccacc gcgcgtgtga agaccctgcc gcgacccgcg caggtcaaag
     2641 ggcttaaggc tgtgtcgcgc gaggtgcgct gtgtgccgtt gaagtggacg ccgagtcccg
     2701 aagccgatgt gacgcactac accgtgttcc gggcgcgttc gtctgagggt cccttcgaac
     2761 agatcggcac cgcgcaggga cgcgccgggg cggaatacac cgatggcggg gccaatcccg
     2821 gtaatctcga ggatgagggc acctacttct atttcgttcg ggcggtgaac gtggtgactg
     2881 cggagagtat cgattccgat acggtgcggg ctgtgacccg cgaggtgccg cccgaggtca
     2941 agcaggtcgc cgccgtatcg gcgcggccgc gcgaggtgcc ggtcgagtgg gaggcctcgc
     3001 ccgataccgc tgtggtcggg tatgaggtgt ggcgcaccct cgccggggcc gacgactgga
     3061 ctcaggtggg tcgcgtgaac agtcgcgaga cgacccgttt tcttgatcgg ggcggcgaac
     3121 aggatgccac gcgcctgggt aagctgcaag acgggaccga ataccaatac cgggtggtgg
     3181 ccttcaacac ggccacggtc cgctcgtccg cctccgtgcc cgcgcccgcc aagaccaagg
     3241 tgatcccggt gccgccctcc ggactcgtgg caaccaccaa tctgccgcat gcgatccggt
     3301 tgacttggag tccgaatccc gagccggatg ttgacggcta ccgtctcgaa gcttccaaaa
     3361 aaccggatgg aggattcagg cgatgggtgg agttcaggct gaacgagaag acggatctga
     3421 gcgcggagga gaaagagctg gatccctcgc aagtccgcta ctaccgtgtg atggcactgg
     3481 accgtgaggg cttggaaagc ctgtggagcg agccgatcga gggccgggcc aaaccgttgc
     3541 cggatccgcc gttcgacttg caggcggaga ccgaaggtgc cttgcggcgc atcacttggc
     3601 agcccccgcc gcagccggac gtggtccggt acgttgtttg ggaaaaatgg tttttgggtt
     3661 ggaagcggct ggccgacacc gataagccgg agtttacgct cacgctgccg ccggatcaga
     3721 aaccgcccac attggctgtg tcggcggtgg atcaggatgg gctcgaaagc cgccgcagtg
     3781 aagcggtgaa tccgtaattt cagtaaccgg gagtgccatg gatacacctt tctcttacct
     3841 cgaatcagcg gcggtgacgg atgtcggccg gcgacgcaag aacaatgaag atgcgctggt
     3901 ggaactcccc gcctgcggag tgttttgtgt ggcggacggc atgggcggcg cgaagggcgg
     3961 ggagatcgcc agccaggcta cggtggaggc cctgcagacg gtgttcgagc attcgacgga
     4021 cgcgcctttc gccgtgaccg ccgcggccgc ggcccggctg gcggcgcgcg ctttgaatca
     4081 ggcaagcgcg tggatccggg agaacgccga ggagcgcggg ttgaccggtt gcggcagtac
     4141 cgccgtgatg ctgctcttcg accggatcaa ccccgcgtgt gcccgtgtgt tgcatgcggg
     4201 tgacagccgc gcctaccgcc tgcggggcgg acggctcgaa cagttgagcc aagaccattc
     4261 cgtggccgct gccgccggac tgaaggatga gaaagatctg cccgccatgt tccgcggggt
     4321 catcacgcgc gcggtcgggt tggagaagtc ggtgcggctc gaggagacgc tggtcgaggt
     4381 gcatggcgct gacctctatc tgctctgctc ggacggactt tctaaaatgg tgagcgatcg
     4441 tcatatccaa aagttgctgc gcaagcacgc tacggagccg atcgggactt tggcgcaacg
     4501 cctaatcgac gaggccctgc atgccggcgg cgaggacaac atctcggtga tcctcgtgcg
     4561 cgtggccgac aaactgccgc cggcgacggt cacggagttg tctccggaaa cgctcgcatt
     4621 agaagctctc gacctcgatg gtgtgcctgc cgccgcagat tctgaagatt ccgagccgct
     4681 cacgcgcgac acccgggaga ccgccgacac cgcaggctgc gaatgcgcca aaacgcccag
     4741 agacatcgac accgatcaag cgatacaaac cccgcccacg cccttttccg gcaccggcat
     4801 tacgccggag gagaatgtgg aaaaggagga ggttgcgccg aagccgcgcc gtcgtctgct
     4861 gttgcgtatt ttggttgtct gtctcatggc gggtctcgcg gccgcggtgt ggatactctt
     4921 ccggacattc cgaccttaat catgatgaag gagcgacccg tacctcatgg atgagaccct
     4981 caaagagcgg attggcgatt atgaattgct ggagctgatt ggagacggcg cccagggccg
     5041 ggtctacaag gcccggcata tggccggtcc ggaaggcgaa ccgcaagagt tggttgcgct
     5101 caaggtcgtg cgtatcaccg gcgaggacga gaagctgcgg ctcaggtttc aggagcaggc
     5161 cgagattctg cgtcggctca accatgccca tatcgtggct taccgcgatt gcttcacctg
     5221 gcatgccggc gagtgggacg aggcccagtg tctggttatg gaattgctcg aaggcgagac
     5281 gctggcggaa ctgctcaagc atgcctcttc cggactgcct tggccgcagg tggaacagat
     5341 cttcgagcaa tgcctcgccg gactgatcca cgcgcgcgag tgcggcatca cccaccgcga
     5401 catcaagcct tccaacattt tcctcaccaa gggcggtcaa gccaagatca tcgacttcga
     5461 tatcgcgcgg cgcgatgatt ccggacagat gtccaccgcc ggttggaagg gcacgtttga
     5521 ttacatggcg ccggatttca tctcgatcga cgggttccgt ggtgacgaga cctccgatgt
     5581 gttcagtctg ggcatctgct tctatcaggc gctcaccggt tctctgccgt atgggaagct
     5641 gggtgaggcc gcgcatatcg gatatctcaa ccggtggcgc gatcaggccg cccccgtgcc
     5701 gtcgttccgg cccggcgtgt tccgtgtgct ggcccatgcc aaaacggttg tttccaaagc
     5761 catcgccgcc cgtcgcgaac aacgttacgc cactttcgcg gccttcctcg aagagttccg
     5821 catgatccgc taccgtcgga tccgaaacaa gaataaggat gagtatgaac tcctcgcggt
     5881 ccttggccgc ggcgggttcg gtgaggtctt caaagcgcgc cgcgtcagcg acgggttggt
     5941 ggtggccgtc aagttcctct tttcggagaa gcagtccgac cgcttcctca aagaggcgaa
     6001 gatcctgcag cagtatccgc atcccaatct ggtccaatat gtcgatttca ttgtcatcaa
     6061 gggagtcacc ggcgagaagc agttctttct ggtcatggag tttctggagg gtatgccggg
     6121 atggaccctg cgggtgcgac ttaaaaacga gggcaagctg gaagtggagg aggcggtcac
     6181 gctcttcaac tgttacctct cggccctgca gttcctccac ggcagcgcgc gccccatcat
     6241 ccaccgtgat atcaagcctg ggaacctcta tgcgccggtc gggcaaccgg agaagggcaa
     6301 ggtctttgac ctcggcgtgg cgcgggatgt ctcgggtacg gtcacggtcg gtggcgtgcc
     6361 gggcactttg gattatatgg ccccggagtt cgcggatgcg ggcggggatc gcggttcgcc
     6421 tcagtccgac ctctatgcgc tcggtctctg cctctatgag gtgctggcgg gcaagccggt
     6481 gttcgaacgt ctgccgggtg acctcaactc cgcctgggtc gcatttcagg accggctgcg
     6541 caaaccgccg gagattgctt tcgacgccga ggtgttcgtg cgctacccgc ggttcaaagg
     6601 ggtgatcctt cgcgcgctgg ccctgaaacc cgacgaccgg ttccccagtg ccgatgcgat
     6661 gcgcaaggct ctgcaggagg cgttactctc tgggagcgcg ccgcaggcgg atgaggggtt
     6721 tgacgcggac gaagggctta ccatggccac cttcgtcggg gcgcggcctg ccgaaccgga
     6781 gccgggtgcc acaaccggaa cccgtgccgt gtcgtccgag gtgcttcagg aagcgctgga
     6841 tgagcaggcg cggcaggcgg accgacggcc gcgcgagcgc gcgggtcgga gaggccccgg
     6901 ctacctgatc gccgcgatcg ccggaacggc ggttaccatg acccttcttt tcggtctcgt
     6961 cgtttattgg ctggggcggg agaagcctaa agccggtcag accgcgcgtg tggagaaacc
     7021 ggctacgccg accgcatcag ccccagcaga aacgaagccc gcgccgaccc cggaggttgc
     7081 cgccgcgaaa cccgctgccg tcgcgcagcc ggccgagcct tc
//
LOCUS       DAOSRB010000146         7087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9869_length_7087_cov_6.281286, whole genome shotgun sequence.
ACCESSION   DAOSRB010000146 DAOSRB010000000
VERSION     DAOSRB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9869_length_7087_cov_6.281286"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLZ74_08150"
     CDS             complement(<1..273)
                     /locus_tag="PLZ74_08150"
                     /inference="COORDINATES: protein motif:HMM:NF018654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HOR98333.1"
                     /translation="MGRARKAEIGDRMVKRFPLYLRVLREMAEQDKPYASGAVVAKAL
                     SLDPIVVRKDFSRTGVRGTPRLGFPLREVIAAIERFLGWNAHTEAVL"
     gene            complement(327..623)
                     /locus_tag="PLZ74_08155"
     CDS             complement(327..623)
                     /locus_tag="PLZ74_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOR98334.1"
                     /translation="MKLVMAVYNIGVDEEVMAAMEQEGVVCFTKIPRVVGKGKATGPR
                     LDNSVWPGANTLSLFVVPDERAQDVMRVFEKLRATIGRKAGLKAFLLHVEQQTA"
     gene            complement(779..2845)
                     /locus_tag="PLZ74_08160"
     CDS             complement(779..2845)
                     /locus_tag="PLZ74_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98335.1"
                     /translation="MRCLILMVCAAVFLNAQADSDAGHAASVTWRVHEDQWSNDLKFA
                     ELTNSLTAYRLTGKIALFTCLSHVPPKPEFFFPLMPILKGRLATLKAMGYRAGINHLC
                     TVGQADEDLASAAVVPGAQYFTNWRGEIAKSNRCENDPVWREKWIKPVYKALAETGPD
                     FIWMDDDLRLLNHGVKGTGCFCPVCMARIRERLGYTGEDVKGLGAFFADPQKGETRRL
                     AMLQLNRETHVSLFTFIRDVVRSIDPKIVLGVMDAGGPRYDGNGYVDYINALAPHGEE
                     VFFRPGAGFYTDQFGVDGILSKANSLAFEAVRIPDTVRCVESEIETFNYQVIGKSTHT
                     LKMEALVYLAAGVRGTAWNVMPYAANDAIAPFARRLKALEAIRSEADAIVAAAGSARP
                     CGVWDGTSENSFAGNRAAGREGAWLDYRVLSGFLGSDLQKIGIPVAYRESEADICAPT
                     SDTVWSWTSEELKRRLAGALFLDVGALKALIDRGHGSDTGFSIGKSFRLDARERYVEH
                     PINQESYGFERDVRQSFWGGPAYELKPHPGAQITGQLVDMRGKEFAPCCSGVFENKFG
                     GRIYASGYFPYSRIGFTPTIRHYHRVFDWLAREGMSGRVDSIHRTAMFVRGSRLVSVF
                     NMTYDTAEDVEILLRGAAWRNGIRSLSDVGNVIKGVPEGVYTRYRLNAAVPAWSLAGF
                     VPVDVK"
     gene            complement(2876..3640)
                     /locus_tag="PLZ74_08165"
     CDS             complement(2876..3640)
                     /locus_tag="PLZ74_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOR98336.1"
                     /translation="MKLNVCFCVALCSVSAIQAAEPVEVTPVAKMDCRTNDRTREPGI
                     FGDYWWANRFLSRHRCIEALRGKTVDVVMIGDSITHFWEWRHPENWLKFTTGRTVLNL
                     GYGGDRTENVIWRLTHGELDGYTAKNVVIEIGTNNNSGHAISTNVANGIVKIIDIVKE
                     HQPTARIILHPIFPRGASERNRGHAYARWRNDRTNELLQKWMAMRPDVVWLDFNAKFL
                     DATGWVPKALMADEIHPTDAGYDIWSAALAPVLKTE"
     gene            complement(3661..6585)
                     /locus_tag="PLZ74_08170"
     CDS             complement(3661..6585)
                     /locus_tag="PLZ74_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98337.1"
                     /translation="MLKKIVLVFCATAASVLFSGTSYTWQGGGSGDWNQPSNWSPQGT
                     PGGDDVAVFSANANLTGDVVIGSAKLTVQVATDKTVVLSGAFSGAGGFVSSGSGTLVL
                     SGNNSFAGNCEVKTGTLLLQSDTAAGAAAVSLSSNGKLIVDTDVTISNNIEIAYAGTP
                     FTLRDGSAVGSVNVGYNYYCTLQVADGGTAHIRAIGGGTQSALSFRSAARYVIDQTFT
                     SSGNLNIDKHTSPVIELAAGGSKFKSWANFVSGRIVFDTVNGFQPSFGWHFSGANATS
                     FFDMNGNSQTISSLGFGADLTGHGYGVTSAAPAMATWNLAKDIVFRGQFTGAAGLDWA
                     PTDGNTLTISGVGWPTRGTLKVSSGTVAVESGVTATNLAEIIVTSGAELTVDALGADI
                     AANALSIAEGALLTLGNGVMLKVSEGQIGSVELEEGRTYSNTDFPGIIMGGGFVYCRS
                     ADDPGPTSGSAVWTGEALSGDAAREADNWKNASLPNFTSGGWSLQFAESGTSATFPSG
                     ESSFNAISLDAPGDFRIASASPYARLVAQGLTLADNLSEPPDPPAMKTYALDMPMTLI
                     TPLSLVASSQESAGDVTLTAAGGLDVRGAVATYQTSLYVEGPYVLNLRGTNRFEKGMY
                     VRRGVVHAYNGDALGKEGVTIWLRSPYNPALHLHGGVYSNPLYFQADSISLFADEGVN
                     TISNEVSGVTTPFRCRAESGAELRFCDHLYGGHGFYPESASDGWIVLAGTRPYRFTTL
                     SIAQYCNLRISVPNNTNTANVVTFRAGSKIFLDVPHAFANKPGLTLKEGAVLDLQGNP
                     QEVGTLAGDEGLDTKLTSATSATFDFMQSTAATNGILIEGGVSLVKRGPETASFSAQS
                     STTGTLSIVEGRFEILPDGGFTNATQVAVAGTGVLAAADNTEGECVFGKDTRLSLDAE
                     GGSIEIPADRRLRVAELYLGETKMPNGTYGRLTGPEAIRTRLVGDGVLYVAGKGFCML
                     IR"
     gene            6836..7045
                     /locus_tag="PLZ74_08175"
     CDS             6836..7045
                     /locus_tag="PLZ74_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98338.1"
                     /translation="MANVHVLQITIAIGIGIAIGIGIGIGFGIGFGFAIGFEIDCCAH
                     AVARSDKCNDGGDSDPDPDGAAMEP"
BASE COUNT         1596 a         2207 c         1794 g         1490 t
ORIGIN      
        1 cagcaccgct tcggtatggg cgttccagcc gagaaaacgt tcgatggcgg cgatcacctc
       61 acgaagcgga aaccccaagc gcggcgtgcc gcgcacaccg gtccgggaga aatctttgcg
      121 caccacgatc ggatccagcg acagcgcttt ggcaactacc gctccggaag catagggttt
      181 gtcctgctcc gccatctccc gcaaaacccg gagatacaag ggaaatcgtt tcaccatccg
      241 gtcgccgatc tcggccttgc gcgctctgcc catgttgtgc ccgacccttt ccgcggttcg
      301 ccgcaaacgg ccacgtaccc acacctttaa gcggtttgct gctccacatg gagaagaaat
      361 gccttaaggc ccgctttacg cccgatggtg gcacggagtt tttcgaaaac ccgcatcaca
      421 tcctgcgcgc gctcatccgg caccacgaac agcgacagcg tgttcgcacc cggccatacg
      481 ctgttatcca gccgcggccc ggtagccttg ccttttccca caacccgcgg gatcttggtg
      541 aagcagacga ccccctcctg ttccatcgcc gccatgacct cctcatccac cccgatattg
      601 taaaccgcca tcaccagttt catcgcatcc cgcccatttt tctacgcgga catctcaatc
      661 gtcctgaatt gtaccaaaga acatctttgg cttcaatcgg tgcgctcgcc tgaggtgcat
      721 tcctgttgtc ttgaactgtg atcagacggc gaaagttcgt aaaccgactg tccggggttc
      781 acttgacatc cacaggcaca aaacccgcga gcgaccacgc cggtaccgcc gcattcaatc
      841 gataccgcgt gtaaacacct tccggcacgc ccttgatcac gttaccgaca tcggacagtg
      901 aacggatacc gtttcgccat gccgcaccgc gcagaaggat ttcgacgtct tcagctgtgt
      961 cgtacgtcat gttgaaaacc gacacgaggc gtgaaccccg cacaaacatc gctgtccgat
     1021 gaatactgtc cacacgtcca ctcattccct cacgcgcaag ccagtcaaaa acgcgatggt
     1081 aatgacgtat cgttggggtg aatccgatgc gcgaatacgg gaaataacct gaagcgtaaa
     1141 tccgtcctcc gaatttattt tcaaaaacac ccgaacagca cggcgcaaat tccttacccc
     1201 gcatatcgac caactgcccc gtgatttgcg ctccgggatg cggcttcaat tcataggctg
     1261 gccctcccca aaaagactga cgcacatctc gctcaaaccc gtaactttcc tgattgatcg
     1321 gatgctccac atagcgctca cgggcgtcaa gccggaaaga tttgccaatg gaaaaccccg
     1381 tgtctgatcc atgaccgcgg tcaatcaacg ccttcaaagc accaacatcc agaaacaacg
     1441 cgccggcaag acgacgcttg agttcttccg acgtccagct ccatacggtg tcggatgtcg
     1501 gcgcacagat gtctgcttcc gattcgcgat atgccactgg aatgccgatt ttctgcagat
     1561 cgctccccag gaacccgctc agcacgcgat aatccagcca cgcaccttca cgtcccgccg
     1621 cacggttgcc cgcaaacgaa ttttccgaag tgccgtccca gacgccgcac ggacgcgccg
     1681 agcctgccgc cgcaacaatg gcatccgctt cactgcggat ggcctccaac gcttttagac
     1741 gccgcgcgaa cggcgcgatc gcatcattgg ccgcatacgg catcacattc cacgccgtgc
     1801 cgcgtacgcc ggctgccaga taaacgaggg cctccatttt cagcgtatgc gtacttttac
     1861 caatcacctg atagttgaag gtttcgatct cggattccac gcaacgcacc gtatccggta
     1921 tgcgaacagc ctcaaacgcg agagagttcg ctttcgagag aatgccgtcc acgccgaatt
     1981 gatccgtgta aaatccggct ccgggtcgaa agaacacctc ttccccatgt ggtgcaaggg
     2041 catttatgta atccacatag ccattgccat cgtaacgagg cccgcccgcg tccatcaccc
     2101 ctaaaacgat cttcggatcg atgctgcgca caacatcgcg aatgaacgta aaaagggata
     2161 catgtgtctc tctgttaagt tgcagcatcg caagccgccg cgtctcgcct ttttggggat
     2221 cggcgaaaaa cgcacccagt cctttcacat cctcgcccgt ataacccaaa cgctcgcgga
     2281 tacgcgccat gcacacgggg cagaaacacc cggtgccctt cactccatga tttagaagac
     2341 gcaggtcgtc gtccatccaa atgaaatccg gccctgtttc cgcaagcgcc ttataaaccg
     2401 gcttgatcca tttttcccgc catacgggat cgttctcgca acggttcgac ttcgctattt
     2461 caccacgcca attggtgaag tattgagcac ccggcacgac agcagcggaa gcgaggtcct
     2521 catccgcctg ccccacggtg caaaggtggt taatccccgc gcggtagccc attgccttca
     2581 gcgtcgcgag acgccctttg aggatgggca tcaagggaaa aaagaactcg ggttttggcg
     2641 gcacatggct caaacatgtg aaaagcgcga tcttgccggt gagacggtac gccgtcagac
     2701 tgttcgtcaa ctccgcgaat ttcaaatcgt ttgaccattg gtcttcatgt acgcgccacg
     2761 tcacactcgc ggcgtgtccc gcatccgagt ccgcttgcgc gttcaagaaa acggccgcgc
     2821 acaccatcag tatcagacat ctcattttcg ttccccgcaa tgattggcga caacgtcact
     2881 cagtcttgag cacaggagcc aacgcggctg accaaatatc gtatcccgcg tccgtgggat
     2941 ggatctcatc ggccataagc gctttcggca cccagccagt cgcatccaaa aactttgcgt
     3001 tgaaatccag ccaaacgacg tcggggcgca tggccatcca tttttgcaga agttcattgg
     3061 tgcggtcatt ccgccaacgc gcgtacgcat gtccccgatt ccgctccgac gccccgcgtg
     3121 gaaagatcgg atgcagtata atgcgcgctg tgggctgatg ctctttcacg atgtcaatga
     3181 ttttgacaat gccgtttgcc acatttgtgg atatcgcgtg cccggaatta ttgttcgtgc
     3241 cgatttcgat caccacgttc ttcgccgtgt agccatctaa ttcaccatgg gtgagccgcc
     3301 atatcacgtt ctctgtcctg tctccaccgt aaccgaggtt cagcacggtg cgcccggttg
     3361 tgaatttcag ccaattctcg ggatgacgcc actcccagaa atgggtgatg gaatcgccga
     3421 tcatgaccac atcgaccgtc ttcccgcgga gagcctctat acatcggtga cgacttagaa
     3481 atcggttggc ccaccagtag tcgccgaaaa taccgggctc gcgcgtccga tcattcgtgc
     3541 ggcaatccat tttcgccacg ggcgtcacct cgaccggctc tgccgcttga atcgcagaaa
     3601 cggagcacaa ggcaacacaa aagcacacat tcagtttcat ctgtccatac caccccgctg
     3661 tcagcggatc aacatgcaaa agcccttgcc ggccacgtat aacacaccgt cacctacgag
     3721 tcgcgtgcgg atcgcttctg gacctgttaa ccggccatac gttccgttcg gcatcttcgt
     3781 ctcaccaagg tacaattccg ccacacgtaa acgacggtcc gcgggaatct ctatcgagcc
     3841 tccctcggca tctaatgaca accttgtgtc ctttccgaat acacactccc cttcagtgtt
     3901 gtcggccgca gccaaaacgc cggttcccgc aacggcaacc tgtgttgcat tggtgaagcc
     3961 gccgtcgggg agaatctcaa accgtccttc cacgatagac agggttcctg tcgtggacga
     4021 ctgtgcggag aaactggccg tctccgggcc gcgcttgacg agcgagacgc ccccctcaat
     4081 caagatgccg tttgtcgccg ctgtcgactg catgaaatcg aacgtggcgg acgtcgcact
     4141 agtcaacttt gtgtccagcc cctcatcccc ggccaaggtg cccacttctt gcggattgcc
     4201 ttgcaaatct agcaccgcgc cctctttcaa cgttaaaccg ggcttgttgg cgaacgcatg
     4261 cggcacgtcc aggaagatct tggaaccggc gcggaatgtg accacattcg ccgtgttcgt
     4321 gttgttcggc acgctgatac gcagattgca atactgcgcg atagataggg tcgtaaatct
     4381 gtacggacgc gttcccgcaa gcacgatcca accatctgat gccgattccg ggtagaaccc
     4441 atgtccgcca tacaggtgat cgcagaatcg gagttcggca ccggattcgg ctctgcatct
     4501 aaacggcgtg gtgactccgc tcacttcgtt gctgatggtg ttcacgccct cgtccgcaaa
     4561 caacgaaatc gaatcggcct gaaaataaag cggattggag tagactccgc catgcaggtg
     4621 aagtgcggga ttgtaaggtg acctgagcca tatcgttaca ccttccttgc cgagggcgtc
     4681 cccgttgtac gcgtgcacga cgccgcgacg aacgtacata cccttctcaa atcgattcgt
     4741 tcccctcagg ttcagcacat aggggccttc gacgtaaaga gacgtctgat aggttgcgac
     4801 ggcaccgcgc acgtccaaac cgcctgcggc tgtcaacgtc acgtcacctg cggattcctg
     4861 tgaagacgcg acaagactta acggagtgat taacgtcata ggcatgtcca gcgcgtacgt
     4921 cttcattgcg ggtggatcag gcggttcgga taagttgtcc gcaagcgtca atccttgtgc
     4981 cacaagccgt gcgtagggcg atgccgaagc aatccggaaa tcaccggggg catcaagcga
     5041 aatggcattg aaggaagatt cgccggaagg gaaagtagcg gacgtgccgg actctgcaaa
     5101 ctggaggctc catccgccgg atgtgaaatt cggcaagcta gcgttcttcc agttgtcagc
     5161 ctcccgcgcg gcatcaccac tgagcgcttc gcccgtccac accgccgatc cggacgttgg
     5221 gccgggatca tcggcactgc ggcaatagac gaagcctccc cccatgatga taccgggaaa
     5281 gtccgtattg gaatacgttc tgccctcttc aagttccact gagccgatct gcccttccga
     5341 aactttcaac atcaccccgt tacccagcgt cagcagcgca ccttccgcaa tcgaaagcgc
     5401 gtttgccgca atatccgctc cgagcgcgtc cacggtcagt tcagccccag aggtgacgat
     5461 gatctcggca agattcgtgg ccgtgacacc cgactcgacc gccacagttc cggaagacac
     5521 cttcaatgtc ccgcgcgtag gccacccgac gccggaaatc gtcaatgtat tcccgtccgt
     5581 cggcgcccaa tccagaccgg ccgcgccggt aaattgtcca cggaacacga tgtcctttgc
     5641 aaggttccat gttgccatag ctggtgcggc gctcgtcacg ccataaccat gtcccgtgag
     5701 gtcggcgccg aaccccaatg aggagatggt ttgcgagttt ccgttcatat cgaagaatga
     5761 cgtggcattg gcgccactga aatgccaacc aaacgagggt tgaaatccgt tcacggtgtc
     5821 gaacacgatg cgtccggaaa caaaattcgc ccacgatttg aacttggatc cgcctgcagc
     5881 aagctcgatg accgggctgg tgtgtttgtc gatattgaga ttgccggacg aggtgaatgt
     5941 ctgatcaatc acataacggg ccgcggagcg aaaggacagc gcactctgcg ttccgccgcc
     6001 gattgcgcgg atatgtgccg tcccgccatc tgcaacctga agcgtacagt aatagttgta
     6061 tcccacgttg accgaaccaa ccgccgaacc gtcgcggagc gtgaacggcg tacccgcgta
     6121 ggcgatctcg atgttattgg aaatcgtgac atcggtatcc acgatcagct ttccatttga
     6181 ggacaagctg acggcagccg cgccagccgc tgtatcggat tgaagaagaa gcgttcccgt
     6241 cttcacctcg caatttccgg caaacgaatt gttccccgac agaaccaatg tgccggaacc
     6301 ggatgagacg aacccccctg cacccgaaaa cgccccagac aatacgaccg ttttgtcggt
     6361 cgccacttgc acggtcaact tagcggatcc aatgacgacg tcacccgtca aattagcgtt
     6421 agcggagaag accgccacat cgtctccgcc cggcgttccc tgcggactcc aattcgacgg
     6481 ctggttccaa tctcccgaac cccccccctg ccacgtgtaa gaagtccccg agaagagaac
     6541 cgacgcagcg gttgcacaga acaccaacac tatttttttc agcatgtgaa acctcctgta
     6601 acgtgattgc tgttactttt atacaggatg gaacggaacc atacaatgcg caattgcgca
     6661 aaataaaatg taaaaaacgc gcaatatttt tgatggggtg catctctctt gtggcctacg
     6721 gagacacgta cactacttta tgacaagccc ttgacgtgta acggtgggta atcggtcagt
     6781 gacggggggg aagagtcatc gtgtacgggt acgtgaacga gtacgtgtac gggatatggc
     6841 caacgttcac gttcttcaaa tcactatcgc tatcggtatc ggtatcgcta tcggtatcgg
     6901 tatcggtatc gggttcggta tcgggttcgg gttcgctatc gggttcgaaa tcgactgttg
     6961 cgcccacgca gtggcccgca gtgacaagtg taatgatggc ggcgattccg atcccgatcc
     7021 cgatggcgcc gcaatggaac cctgaaacgc atgaccttcg atcgcacccc tgtcacgaac
     7081 cgattac
//
LOCUS       DAOSRB010000147         7084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9870_length_7084_cov_6.460663, whole genome shotgun sequence.
ACCESSION   DAOSRB010000147 DAOSRB010000000
VERSION     DAOSRB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9870_length_7084_cov_6.460663"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLZ74_08180"
     CDS             <1..276
                     /locus_tag="PLZ74_08180"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOR98339.1"
                     /translation="PHLIKLRGGRLLTVYGRRLGPGFGEYACLSDDHGRTWDVANEIK
                     LAGHFNGDLGYPASVQLPDDTILTVYYQAEKQGEKPCLMATRWRVRE"
     gene            361..1968
                     /locus_tag="PLZ74_08185"
     CDS             361..1968
                     /locus_tag="PLZ74_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98340.1"
                     /translation="MLKTSKGMLVTLLAVGWLSRDTLAGEDVRVETNKLACMVRYEDF
                     GARGDGRTDDTAAIVRAHAYANRRGLPVQANASATYYIGGQDTPVIIQTDTDLGSARF
                     IIDDRKVENRHTHVFSVYSALPSTQPKGITSLKSGQRRINIELPKTCVVCVTDSNTKR
                     YIRRGLNQNAGSSQTDVFLVDKNGRVDERTPILWDFDQITHMVAYPVDEAPLRINGGR
                     FTTLANAAESEYIYYARGISIRRSNVVIDGLEHRITGEGDHGAPYAGFITIRDCANVT
                     VRNTTLNGHKTYQTIGSAGKPVFMGSYDISVTRALNVSFINCRQFDEINDHTRWGIMG
                     SNYSKNLLYDRCELSRFDAHQGVAGVTIRNSVLGHAGINAIGSGMLVVENTTVYGRSF
                     INLRSDYGSTWQGEFVIRNCTFVPAGGRPVSASLIGGSNDGQHDFGYACCMPSRIDIN
                     GLRIDDSRHPKGYQGPTVFADFNRSFKNAAYEEKFPYVKSREVILENITTVSGKPLHI
                     SSNPVMFKDVAVKHNGKANKPAGGDSQ"
     gene            2072..3652
                     /locus_tag="PLZ74_08190"
     CDS             2072..3652
                     /locus_tag="PLZ74_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR98341.1"
                     /translation="MLRYRAVLTRIAAVGLAGWVARAQSPFTLTLESTAVTPEHAEVR
                     VVLGVPEKHLIYAETFRVSAEPVRVPQAETKPDPIDPSRRVGVYAKTFESLWRVAPLR
                     DGTELTVAYQGCDDQVCFLPEEHVFRFDRQTGRFVATVANADAEAERTVDRFWTQGLR
                     MVTGGGYMGTGAFLAFLDQAETGTSGTAPAGNGWGGFLRDPVAFFHAHGLALTLVLVL
                     IGGLLLNLTPCVLPMIPINLAILGAGAGTRGRGFALGAAYGAGIVLVYGGLGWLILRT
                     GLFFGALQASPWFSLAVALIFTALTLALFDVFVIDFTRFFTPKNGGARQGALAAFSAG
                     ALSALLAGACVAPVVLAVLLLAGTLYAGGATGAQMLPFVLGLGMALPWPFAGAGLSVL
                     PAPGLWMVRVKQAFGVFLALLAAYYFHLAAVGFRSGGDAVAREGSIAAGDRAAWEAAV
                     ARARRAGKPIFVDFWATWCKNCTVMERTTFADERVRARLATYEVVKVQAEKPDREPAR
                     GMLEAFGVRGLPGFAVLK"
     gene            3736..4893
                     /locus_tag="PLZ74_08195"
     CDS             3736..4893
                     /locus_tag="PLZ74_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOR98342.1"
                     /translation="MAKRGFNIDPVTLRSMPFWCVGNPLGDPFGGPVLPKIDSLEVAE
                     LLAEAAGEGLIEATSFHDDDLVPWDPAKPEDDLDPKSETYAKLRAIKRVLDKAGLKTN
                     VATCGLHGDKLFRKGGLTNPDPKVRALAKKKVQRALRIGALFGAKHFTYWVARDGFEV
                     PVNVDWKNVYKWLADGLNGVYDYIRKMKFKNYIGATIEPKPNEPRGHMFLPTSGHAVG
                     FIYGHLKHPEFYGVNPELLQHESMTLLNAVHTVGYLTSMKKLFFLHFGSQIKGQFDND
                     FPPLIGPEGLKETVQMFWALRKIGWKGVVEYDCHMLRTDGDPDDPIGCRRAFIRDCTR
                     ALAMALLLADRLMDFDPAGMRDAEADLTATAMLCRLDPKSIRSWMTTVPGL"
     gene            4890..5699
                     /locus_tag="PLZ74_08200"
     CDS             4890..5699
                     /locus_tag="PLZ74_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOR98343.1"
                     /translation="MILHAESSGSGPDVVILHGLMGSCENWRSVRKILAGHYRVACLD
                     LPNHGRSPHVAQMEARAIGKMVLSTLDAFGMNRAVVVGHSLGGKIAMQMASEHADRLA
                     GVAVVDISPRAFQPMHLFILRACQQLDLVAAVRRVDLDHALAASVPHAETRAFLLKNV
                     VRDPKGRWAWRVPLQTLIDNYRVVSDAPPLVAPYNGPTLFVAGECSPFRIRDDESLIR
                     SWFPAVRWVTIPNAGHLVHADQPEAFTAALAGFLGTCYPSAELNQTPSAEV"
     gene            complement(5675..6556)
                     /locus_tag="PLZ74_08205"
     CDS             complement(5675..6556)
                     /locus_tag="PLZ74_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOR98344.1"
                     /translation="MRLFDAHTHLHDSRLASVFDAVIQKAEEAGITGLCSCGTAPVDW
                     ADVASLAERAGPFHIVPAFGVHPWFVAKLPDDWLEQLEPFLMRYANAPVGEIGLDGVR
                     REIPAARQEEVFVRQLDLAVRLQRPVVLHGARAWEAVLRTLKPYASRLPGILAHGFSG
                     SAEHLSRLLELGGHVSLGGTVCNPQAATVRTVAQCVPEDRLLLETDSPDLLPQTGLSA
                     GLGPDGKPLNQPSNLMRVCEAVAALRDLDPLHLAERTYANALAFFPQTAAGPCGRPLP
                     ARTSRVCVPTYTSALGV"
BASE COUNT         1335 a         2008 c         2322 g         1419 t
ORIGIN      
        1 ccgcacctga tcaagttgcg tggcggccgt ctgctgacgg tctacggccg caggctcggc
       61 cccggcttcg gtgagtatgc gtgcctcagt gacgatcacg ggcggacgtg ggatgttgcc
      121 aacgaaatca agttggctgg ccacttcaac ggcgatctcg gttatcccgc ttcggttcag
      181 cttccggatg acacgatcct tacggtctac tatcaggcag agaagcaggg ggagaagcct
      241 tgcctgatgg cgacgcggtg gcgtgtcagg gagtgacgtg aacggaacga caacgaatcg
      301 gttttccgcc ggaatcatgt cgcggttagc agcggaaggg taggggaagt tgtttttcat
      361 atgttgaaaa caagcaaagg catgctggtt acgctactag ccgtcggatg gcttagtcgg
      421 gataccctcg cgggagagga cgtgagggtg gaaacgaaca agttggcgtg catggtgcga
      481 tacgaggatt tcggggcacg gggggacggc aggacggatg acaccgcggc gattgtccga
      541 gcgcatgcgt atgccaaccg gcgggggttg ccggtacagg cgaatgccag cgcgacctac
      601 tacatcgggg gccaagacac cccggtgatc atccagacgg acaccgactt ggggagcgcc
      661 cgattcatca ttgatgatcg caaagtggag aaccgtcaca cgcacgtgtt ttccgtttac
      721 tcagcgttgc cgtccacgca acccaagggg atcacttctt tgaagtcggg ccagcgcaga
      781 ataaacattg agctgcccaa gacctgtgtg gtgtgcgtca cggattccaa caccaagcgc
      841 tacatccgga ggggactgaa tcagaatgcc ggaagttcgc aaaccgatgt gtttctcgtc
      901 gataagaatg gcagggttga tgagcgtacc cccatcttat gggattttga ccagattacc
      961 cacatggtcg cctatccggt cgatgaggcc ccgctgagga tcaacggtgg ccgttttaca
     1021 acccttgcca atgccgcaga gtcggaatac atctattatg cacggggaat ctcaatccga
     1081 cgctccaatg tggtgatcga cggtcttgag caccgcatca ccggcgaagg cgatcacggt
     1141 gcaccgtacg ccggattcat caccatccgc gactgcgcca atgtgacggt gcgcaacacc
     1201 acgctgaacg gacacaagac ctaccagacg atcggcagtg cgggcaagcc ggtgttcatg
     1261 ggcagttacg atatttccgt tactcgagcc ttgaacgtgt cctttataaa ctgtcgccag
     1321 ttcgatgaga tcaacgacca cacccgctgg gggatcatgg ggtcgaacta cagcaaaaat
     1381 ctcttgtatg atcgatgtga actgtcgcga ttcgacgccc atcagggtgt ggccggggtg
     1441 acgattcgca actcggttct ggggcatgcg ggaataaatg ccatcggcag cgggatgctc
     1501 gtcgtggaga acaccaccgt ctacggcagg tcgttcataa atttgcgttc tgactacggc
     1561 agtacttggc aaggtgagtt tgtcattcgg aactgcacat ttgttcccgc aggcggcagg
     1621 ccggtcagcg ccagcctgat tggcggttca aatgacgggc aacatgactt tggatacgcc
     1681 tgctgcatgc caagtcgaat cgatatcaac gggctgcgta tcgatgactc ccggcatccg
     1741 aagggctatc aggggccaac tgttttcgca gacttcaatc ggagcttcaa aaacgccgct
     1801 tacgaagaga aattccccta cgtcaaaagt cgtgaagtga tcctcgagaa catcaccacg
     1861 gtaagcggca agccgctcca catcagcagc aatccagtca tgttcaagga tgttgccgta
     1921 aagcacaacg ggaaggccaa caagcccgct ggcggcgact cccaataact tgcgtcttcg
     1981 tgttgggcgt ccgttccgca ggtgcgtttt ttggtggaac ccgcaccggc aagtcgctac
     2041 actgaaggaa accgtttatg gagaggtgtg tatgctccgt tatcgcgcag ttctgactcg
     2101 gatcgcggcc gtagggctcg cgggatgggt ggcgcgtgcg caatcgccat tcaccctgac
     2161 cctggagagt acggctgtga cgccggagca cgccgaagtg cgcgtggtgt tgggcgttcc
     2221 ggaaaaacac ctcatttacg cggagacgtt ccgtgtcagt gccgaacccg tgcgggtgcc
     2281 gcaggcggag accaagccgg acccgatcga cccgtcccgg cgggtcggcg tgtatgcgaa
     2341 aaccttcgag tccctatggc gggtcgcgcc gttgcgtgac ggcacggaac tgacggtagc
     2401 gtatcagggt tgcgacgatc aggtgtgttt cctgcccgag gagcatgtgt tccgttttga
     2461 ccggcagaca ggacggtttg tcgcgacggt ggccaatgcg gatgcggaag cggagcggac
     2521 cgtcgatcgt ttctggacgc aggggcttcg gatggtgaca ggcggcggat acatgggaac
     2581 aggggcgttc ctggcgtttt tggatcaggc cgagactgga acaagcggga cggctcccgc
     2641 tggaaacggg tggggcggtt ttctgcgcga cccggtggcc tttttccacg cgcatggatt
     2701 ggcgctgacc ctcgtgctgg tgctgattgg cgggttgctg ctcaatctga cgccatgcgt
     2761 gctgcccatg atcccgatca atctcgcgat cctcggggcc ggggcgggaa cacgcggccg
     2821 cgggttcgcg ttgggtgcgg catatggtgc cgggatcgtg ctggtctacg gcggactggg
     2881 gtggctgatc ctgcgtaccg gactgttttt cggcgcgttg caggcctcgc cgtggttcag
     2941 tctggcggtg gcgctgattt tcactgcgct gacgctggca ctgttcgatg tgttcgtgat
     3001 cgatttcacg cggttcttca cgccgaaaaa cggtggtgcg cgtcagggtg cgctagcggc
     3061 cttctccgca ggcgcgcttt cggccctgct ggccggggct tgcgtcgcgc cggttgtgct
     3121 tgcggtgctg ctgttagccg gaacgctcta tgcgggcggt gccacgggtg cgcagatgtt
     3181 gcctttcgtg ctggggttgg gcatggcctt gccgtggccg ttcgcaggcg ccggactctc
     3241 cgtgctgccc gcgcccggtc tctggatggt ccgtgtgaag caggcctttg gcgtgtttct
     3301 ggccctgttg gcggcgtact acttccacct tgccgccgta gggttccgtt cgggagggga
     3361 tgcggtggcg cgcgaggggt cgatcgcggc cggagaccgc gcggcatggg aagccgcggt
     3421 tgcccgggcg cgccgtgcgg gcaagccgat tttcgttgat ttctgggcca cctggtgtaa
     3481 gaactgcacg gtgatggagc ggacgacgtt cgcggatgag cgtgtgcgcg cgcggctcgc
     3541 aacctatgaa gtggtgaagg tgcaggcaga aaaaccggac cgcgagcccg cgcgcgggat
     3601 gctggaagcg ttcggcgtac gcggactgcc cggtttcgcg gtgttgaagt aagggctcag
     3661 agttgggaag gcggtctgat gcggccgcgg gaaacgcctg tgcaaacggg cacacaggaa
     3721 aacaggagag gcgcaatggc aaaaagagga ttcaatatcg accccgttac acttcgctcc
     3781 atgccgttct ggtgtgtcgg caatccgctg ggtgatccgt tcggcgggcc ggtgctcccg
     3841 aagatcgatt cgctggaggt ggcggaactg ctcgccgaag cggcgggcga gggcttgatc
     3901 gaagcgacgt cgtttcatga tgacgacctc gtgccgtggg atccggcgaa gcccgaagac
     3961 gatctggatc cgaagagcga aacgtacgcc aaactgcgcg cgatcaaacg ggtgttggac
     4021 aaagccggcc tcaagaccaa tgtcgccacc tgcggcctgc acggtgacaa actctttcgt
     4081 aagggtggcc tgaccaaccc tgatccgaag gtccgcgcac tggctaaaaa gaaggtgcag
     4141 cgtgcgctgc gcattggcgc gttgttcgga gccaagcact tcacttactg ggtggcgcgt
     4201 gacggcttcg aggtgcctgt gaatgtggat tggaagaatg tttacaagtg gctggcagac
     4261 ggcttgaacg gcgtctatga ctacatccgg aagatgaagt tcaagaacta catcggcgcg
     4321 acgatcgagc ccaagccgaa cgagccgcgc ggccacatgt tcctgccgac atcgggtcat
     4381 gccgtgggct tcatctatgg gcacttgaag catccggagt tttatggcgt gaatccggag
     4441 cttttgcagc atgagagcat gacgctcctg aatgctgtgc atacggtcgg ttatttgacc
     4501 agcatgaaga aactcttctt cctgcatttc ggcagccaga tcaaagggca gttcgacaac
     4561 gacttcccgc cgctgatcgg ccccgaaggg ctcaaggaaa cggtgcagat gttctgggcg
     4621 ctccgcaaga ttggctggaa aggcgtggtg gaatacgatt gccacatgct gcggaccgat
     4681 ggcgatccgg atgatccgat cgggtgccgt cgcgcgttca tccgggattg cacgcgggcg
     4741 ctggccatgg ccctgctgct ggccgaccgc ctgatggatt tcgatcccgc aggcatgcgg
     4801 gacgcggagg cggatctgac ggctaccgcc atgctgtgcc gccttgatcc gaaatccatc
     4861 cggtcgtgga tgacaaccgt ccccggttta tgatcctgca tgcggagagt agcggatcag
     4921 ggccggacgt ggtgattctg cacgggctga tgggctcgtg cgaaaattgg cggagcgtgc
     4981 gcaagatact ggccgggcac tatcgtgtgg cctgccttga tctgccgaat cacgggcgtt
     5041 cgccccatgt cgcgcagatg gaggcgcgtg cgatcggcaa gatggtgctg tcgacgcttg
     5101 atgccttcgg gatgaaccgt gccgtggtgg tcgggcactc gctgggcggc aagatcgcga
     5161 tgcagatggc ctcggaacat gccgaccgtc tggccggggt ggcggtggtg gatatctcgc
     5221 cgcgcgcgtt tcagccgatg caccttttca tcctgcgggc ctgccagcaa ctggatctgg
     5281 tcgccgccgt gcggcgtgtc gatctggacc acgcgctggc ggcatccgtg ccgcatgccg
     5341 agacacgcgc gttcctgctc aaaaacgtgg tgcgcgatcc gaaggggcgc tgggcctggc
     5401 gcgtgccgct tcagacgctc atcgacaact accgtgtggt gagcgacgcg ccgccgctgg
     5461 ttgcgccgta caacgggccg accctgttcg tggccgggga gtgctcgccg ttccgcatcc
     5521 gtgacgacga gtccctgatc cgcagttggt ttcccgctgt ccggtgggtc accatcccga
     5581 acgccggcca tctggtgcat gccgaccaac ctgaagcctt taccgcggca ttggccgggt
     5641 ttctcggtac ttgttatccg tctgctgagc tgaatcaaac gccgagcgct gaagtatagg
     5701 ttgggacgca tacccgagac gtccgcgcag gcaagggccg gccgcatggg ccggcggcgg
     5761 tttgcggaaa aaacgccaga gcgttggcgt atgtccgttc ggccagatgc aggggatcga
     5821 gatcgcggag ggctgccacc gcttcgcaga cccgcatcag attggagggt tggttcagcg
     5881 gtttgccatc tggacccagc cccgccgaca gtcccgtctg gggcagaaga tccggagagt
     5941 cggtctccag caacagccga tcttcgggaa cgcattgcgc cacggtccgg actgtagcgg
     6001 cttggggatt gcagaccgtc ccgccgagcg acacatggcc tcccagttcg agcagacggc
     6061 tcaaatgctc tgcggatccg ctgaacccgt gcgccaggat accgggtagc cgcgacgcgt
     6121 agggcttgag ggtccgcaac acggcttccc aggcgcgtgc gccgtgcagc accacaggcc
     6181 gctgtagccg caccgccaga tcaagctgcc ggacaaacac ctcctcttgc cgcgcggccg
     6241 gaatttcgcg gcgcaccccg tccagcccga tctcgccgac cggtgcgttc gcgtaccgca
     6301 tcaaaaaggg ttccaattgc tcaagccagt catctggcag ctttgcgacg aaccaggggt
     6361 gcactccgaa agcgggcacg atgtggaacg ggcccgcccg ttccgcaagc gacgccacat
     6421 ctgcccaatc cacaggcgcg gtgccgcagg agcagagtcc ggtaatgccc gcctcctccg
     6481 ccttctggat cacggcatcg aagaccgatg ccagccggga gtcgtgcaga tgggtgtgtg
     6541 catcaaacag tctcatgact ccacgggtta ccttatagag ccagaatcat cgtgccgctg
     6601 tagttcaata ctccaccttg cgtccgtgat cgttgccgcc tcattatacg tcaggtcgta
     6661 aaactgtcaa ttgggggaag atactggttc cgaatcatga aacacatgaa acttcatgaa
     6721 atcaaggacg ggcaaatttt ttggcttcgg gtttcggatt tttggaaccg ccaagacgtc
     6781 aggagcgcgg acggcatggc acaggcatct tgcccgtgtt tcatgggcgg ggcgctcatg
     6841 tcacaaaacc cttcgagccc ttcgtcgctc cactttatcg caagcgcaat cgactgagtt
     6901 ttggagaccg gtgcgtatgc gggactaaag cgtccgccaa ccggtaggga cgcccgcttc
     6961 tcccgtcaga ccttatgtcg acccgcgacc cgcgacgagc gacctgcggt gtcggaaacg
     7021 cggacggctc ctgtcgatcg tcgcgggtcg aatgtcgcgg gtcgcgggtc gacgttcccg
     7081 atcc
//
LOCUS       DAOSRB010000148         7084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9871_length_7084_cov_6.286243, whole genome shotgun sequence.
ACCESSION   DAOSRB010000148 DAOSRB010000000
VERSION     DAOSRB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9871_length_7084_cov_6.286243"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1165)
                     /locus_tag="PLZ74_08210"
     CDS             complement(164..1165)
                     /locus_tag="PLZ74_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOR98345.1"
                     /translation="MSLQRGTYGIEYDERSGTSFRVSRLIVALLPVVAVVVLFARGCR
                     NEGETESDDGKTVGPAHYRLAESETPHERPSLWSSLFQREKPSDAGTAPEPVSGWRTA
                     GVTPPPETLVQSPEIRRLLEQAGACVEEDDLVGARRFLREILLRRDADSVRAFAEREI
                     GRLNIKLLFADRPMPEKVKHRVASGEVVSRLAKRYGNTPELILKANGIDRPERLRIGR
                     EIWVLDAPVFELTVYKNGGSAVLTLNGSFFKRYPLLGGIPVKAPSGTYALRGKVALDG
                     VWRIMLAATGATPEAEGLALHGGADTPATDDRIRFATSDIEELSVLLPVGAAVNIAE"
     gene            complement(1220..1951)
                     /locus_tag="PLZ74_08215"
     CDS             complement(1220..1951)
                     /locus_tag="PLZ74_08215"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOR98346.1"
                     /translation="MVRLGVNIDHVATLRQARGTTYPSLTEAAALCEQAGAHGITIHL
                     REDRRHIQDADVYALRAALKTRLNLEMANNPDVLAVALDVRPDEVCIVPERRQELTTE
                     GGLDAAGLQGQLAPTVAALRAAGILVSLFIEPDERQVEAAAALGAPFIELHTGTFAAR
                     TGEAREAELARLAHAAVRAHEFGLRVNAGHGLTVENVGAILEIPHLDTLNIGHSIVCR
                     AIAVGLAQATREMLERIGGGNREDA"
     gene            complement(2025..5228)
                     /locus_tag="PLZ74_08220"
     CDS             complement(2025..5228)
                     /locus_tag="PLZ74_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOR98347.1"
                     /translation="MNVIGWVVVVVWGLCLCGRGADAREWENEQVNAVNREPARAAGF
                     PLADARAALTPEEPQTPFRVSLNGMWRYQWSGSPDDRPAGFHEPEFDDARWFTLDVPS
                     CVEMRGFGIPIYTNVKYPHARTPPKIGTAYNPVSAYRTRFTVPETWAGRPVFVRFDGV
                     YSAFYLWVNGQYVGYSEDSKLPAEFNLTRYLKPGGNVMAVEVYRWSDGSYLEDQDMFR
                     FSGIYRDVSLFSPPTAELRDFFVIAQPDADCRTAVLRVTSRARALGGRAVAATVRGEL
                     YDAENKPVEAALPETRLTLPADGTDVQAHVESPVGKERPPRLWSAEDPYLYTLVLTLE
                     GDDGSRDIRSCKVGFRRVDIREGKLLFNGRAIKFKGVNRHETSPENGRTISREDMLRD
                     ILLFKRHNIDTVRTAHYPDHYYWYQLCDRYGIYVVAEANVESHGMGYGKESLSHVPSW
                     EKAHVERNVNHVENYKNHPSVVMWSLGNEAGPGKNFKAAYDAVKARDATRPVHYERAN
                     DIVDVDSHMYPTVEWLYERGKNTAKPFFLCEYAHAMGNALGNFKEYWEAFYSSESLSG
                     GCIWDWIDQAVWKETDRVGPDGRRLRYLAYGGDFDDQPNDGNFVCNGVIGPTREITPK
                     LIEVKRVHQNLVVTAENAAAGRVEVWNRFGFTNARVFDMRWSLACDGREIAAGRVDAP
                     DVPPLSRRTVSVPVPKVTPRSGAEYFYRVSFHLREKTAWAETGHEIAASQLAFTPPQA
                     APSPSPAARGPVPGVRVRESRRAVTVSGRRFEAVFSRASGTLASLVYDGRTVICDRAG
                     ITHGPRLNTFRAFVDNDNQFGKWLRAEYMDSGLSQMSHHARPLQVERVDDATVLVRAT
                     VEACGFKSGRFTHEAEYRITGDGAITVRNRITPAGSLPPLPRMGVRMMLDGALERMAY
                     YGRGPWENYVDRNTGSDIGYYTGTVTEQYVPYVRPQECGGKSDVRWAAFLDRNGRGIH
                     FTFPQPLFVTALHYTSEDLEGARHRKGMERLFNPPTPRSEVCLALDIAQMGLGGASCG
                     PIPMEKYILRARPFAYTYIMRPCVKGSHAELSDLARP"
     gene            5624..6916
                     /locus_tag="PLZ74_08225"
     CDS             5624..6916
                     /locus_tag="PLZ74_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOR98348.1"
                     /translation="MYPKYVFLFIGDGMGATHVRATECYLRDAQIQRGVAAGMMKGLI
                     MLALPVQGRATTFAKNKATTDSAASATALSSGYKADNGVLNIDLETERKFDVMAQFVK
                     RQGMKMGVISSAAPNHATPAGFYAHAKNRNMYPEIAMQLAVCGLDYVGGPPMLGVEDT
                     DAKAKKAAANNGHTIVEDRKSFDALKPGVKCVWVRSDMPLVIDGKQEIPLADHVSKAI
                     ELLAGPEGFFMMVEGAAIDWASHSNDGAAMVQELLEFDRAIARAVEFYRARPDEVLIV
                     VTADHECGGLSLIEGPLTAPGMSKILDGQKKSRNASAEMFKKFEKEKITFDEALPQMK
                     AFFGIQHLSDEELAKCREIFDKGGDEQGKHAYGDERRISLFWSHLVSARAGFIWGSLN
                     HTDDPVPVYAVGVQAARFAGSSDNALIGRYLRDLISGK"
BASE COUNT         1335 a         2301 c         2165 g         1283 t
ORIGIN      
        1 tcctccccgc catgctcggt gcccgcgcca cgcatgatca gagcgtcgcg ttcattgaat
       61 ccaagctcaa ggagtggggc ctcccgaccg cctgacaccg caccccgaat cgacccgggt
      121 cgaatcaggg cgaggccgct cgagtcaggt cgccccgatt cgcctactcc gcgatgttca
      181 ccgcagcgcc gacgggcagc agcacactga gttcctcgat atctgaggtg gcgaaccgga
      241 tacggtcgtc cgtggcgggt gtatccgccc cgccgtgcag ggcgagtccc tctgcctcgg
      301 gtgtcgcgcc ggtcgccgcc aacatgatcc gccacacgcc atcaagcgcg actttgccgc
      361 ggagggcata ggtgccggaa ggggccttga ccgggatacc gcccagtagc ggatagcgct
      421 tgaagaagga tccgttcagc gtcagcacgg cactcccgcc gttcttgtac accgtcagtt
      481 cgaacactgg cgcgtcgagg acccagatct cgcggccaat gcgcagacgc tcgggacggt
      541 cgatcccgtt ggccttcaaa atcagctcgg gggtgttccc gtatcgtttg gcgagccgac
      601 tgaccacctc tccggatgcg acgcggtgct tgaccttctc cggcatcggc cggtcggcga
      661 agagcagctt gatgttcagc cgcccgatct cacgctcggc aaacgcgcgc acactgtcgg
      721 catcgcggcg caacaggatc tcgcgcagaa accgccgcgc gcccacgagg tcatcctcct
      781 ccacgcacgc gccggcctgc tccagcaacc ggcggatctc gggcgactgc acaagggttt
      841 ccggcggcgg cgtcacgcct gccgtgcgcc agccggagac cggttccggg gccgtcccgg
      901 catcggacgg tttctcccgc tggaacagcg aagaccagag cgaaggacgc tcgtgcggcg
      961 tttcggactc agccagccgg taatgggccg gaccgaccgt tttcccgtcg tccgactccg
     1021 tttccccctc gttccggcag ccgcgcgcga acagcaccac gaccgcaacc accggcagca
     1081 gcgcgacgat cagccgcgac acccggaagg atgttccgct ccgctcgtcg tattcgatgc
     1141 cgtatgtgcc gcgctgcaaa gacatgtgac cctctccgcc ggagggggaa tgctgccggg
     1201 tccgtgccct ccggacccgt cacgcatcct cccgattgcc gcctccgatc cgttccagca
     1261 tctcgcgtgt ggcctgggca agacccacgg cgatcgcccg gcacacgatg ctatgcccga
     1321 tgttcaacgt atcgagatgc ggtatctcca gaatcgcccc gacgttctcg accgtcaacc
     1381 cgtggcccgc gttgacacgc agtccgaact cgtgtgcgcg gaccgcggca tgcgccagcc
     1441 gcgccaactc cgcttcgcgt gcctcgcccg tgcgcgcggc aaacgtcccg gtatgcaact
     1501 caatgaacgg cgcaccgagc gccgccgcag cctccacctg ccgttcgtcg ggctcgatga
     1561 acagactcac cagaatcccc gccgcgcgca acgcggccac ggtcggcgcg agttgccctt
     1621 gcaggcccgc cgcgtccagc ccgccctcgg tcgtcagttc ctgccggcgc tccggcacga
     1681 tgcacacctc atccggccgc acatcgagcg ctaccgccag cacatcgggg ttgttcgcca
     1741 tctcgaggtt caggcgcgtc ttcagtgccg cgcgtaacgc gtacacgtcg gcatcctgaa
     1801 tgtggcggcg gtcctcgcgc agatggatcg tgatgccgtg cgcacccgcc tgttcacaga
     1861 gcgccgccgc ctccgtcaaa ctcggatacg ttgtcccgcg cgcctgacgc aacgtggcca
     1921 catgatcaat gttgactccc agccgaacca tatggtcctc cttctttacc tcacagcata
     1981 acaccgtttt ccgatcctgt ccccgatttc cgcgccgcgc cgcatcaggg cctggccaaa
     2041 tcgctgagtt ccgcatggct gcccttaacg cagggccgca tgatgtaggt gtacgcaaag
     2101 ggccgggcac gcagaatgta tttctccatg gggatcgggc cgcagctcgc gccgccgaga
     2161 cccatctgcg caatgtccag cgcgaggcag acctcggaac gcggcgttgg cggattgaag
     2221 aggcgctcca tccctttgcg gtggcgcgcc ccctccaagt cctctgaggt gtagtgcaga
     2281 gccgtgacaa aaagcggttg cggaaacgtg aaatgaatcc cgcggccgtt gcggtccaga
     2341 aacgcggccc accggacgtc actcttgccg ccgcactcct ggggccgcac atagggcacg
     2401 tactgttctg tcaccgtgcc ggtgtagtac ccgatgtcgc tccccgtatt acgatccacg
     2461 tagttctccc aagggccgcg cccatagtaa gccatgcgct ccagcgcacc gtccagcatc
     2521 atgcgcacgc ccatgcgcgg cagcgggggc aggctgcccg cgggcgtgat gcggttccgc
     2581 accgtaatcg cgccgtcccc ggtgatgcga tactcggcct cgtgcgtgaa ccggccgctc
     2641 ttgaagccgc atgcctcaac cgtggcccgg acaagcaccg tggcatcgtc cacccgctca
     2701 acctgcagcg gccgcgcgtg atggctcatc tgcgacaggc cggaatccat gtactcggcg
     2761 cgcaaccatt tgccgaattg gttatcgttg tccacaaagg cgcggaaggt gttcagacgc
     2821 ggaccgtgcg tgatccccgc acggtcgcag atcaccgtcc ggccgtcgta caccagagag
     2881 gcgagcgtcc cggatgcccg gctgaagacc gcctcgaagc gccgaccgct cacggtcacc
     2941 gccctgcggc tttcacggac ccggacgccg ggcacggggc cgcgcgcggc cggagacggg
     3001 gacggcgcgg cttgcggcgg ggtgaacgcc aactgactgg ccgcgatctc gtgacccgtt
     3061 tccgcccacg ccgtcttctc gcggagatgg aaactcacgc ggtagaaata ctcggcaccg
     3121 gaccggggcg tcaccttggg aaccggcacg gacacggtgc ggcgcgagag cggcggcaca
     3181 tcgggcgcgt caacccggcc tgcggcgatc tcgcggccgt cgcatgccag agaccagcgc
     3241 atgtcgaaca cgcgtgcgtt ggtgaagccg aagcggttcc agacctcgac ccggcccgcg
     3301 gccgcattct cggccgtgac caccagattc tgatgcacac gcttgacctc gatcagcttc
     3361 ggcgtgatct cacgggtcgg cccgatcacg ccgttgcaga cgaaattgcc gtcgttcggc
     3421 tgatcatcga aatcgccgcc gtaagccaga tagcgcagcc gccggccatc cggcccgacc
     3481 cggtcggtct ctttccacac cgcctggtcg atccagtccc agatgcaccc gccggagagc
     3541 gattcgctgg agtagaaggc ctcccagtat tccttaaagt tgccgagcgc gttgcccatc
     3601 gcatgcgcgt attcgcagag gaagaagggc ttggcggtgt ttttgccgcg ttcgtacaac
     3661 cattcgaccg tcgggtacat gtggctgtcg acatccacga tgtcattggc ccgctcgtag
     3721 tgcacgggcc gcgtggcatc ccgcgccttg acagcgtcgt acgcggcttt gaagttcttg
     3781 ccgggaccgg cctcgttgcc caacgaccac atgacaaccg aaggatggtt cttgtagttc
     3841 tcgacgtggt tcacattacg ttcaacatgc gccttctccc atgagggcac atgactgagc
     3901 gactccttgc cataccccat accgtgcgac tcgacgttcg cctcggccac aacatagata
     3961 ccgtaccggt cgcagagctg ataccagtaa taatggtccg gatagtgcgc cgtgcggacc
     4021 gtgtcgatgt tgtggcgctt gaagagcaga atatcccgca gcatatcctc ccggctgatc
     4081 gtgcggccgt tctccggcga ggtctcgtgg cggttcaccc ccttgaactt gatcgcccgg
     4141 ccgttgaaga ggagcttgcc ctcgcggata tcaacccggc ggaatccgac cttgcaggag
     4201 cggatatccc gcgagccgtc atcaccttcg agcgtgagaa ccagtgtata gaggtacgga
     4261 tcttcagccg accacaaacg ggggggccgt tccttgccta cgggcgactc gacatgcgcc
     4321 tgcacgtcgg ttccgtcggc aggcagcgtc agacgcgtct cgggcaacgc ggcctcgacg
     4381 ggtttgtttt cagcgtcgta caattcgccc cgcaccgtcg cggcaaccgc ccggccgccc
     4441 aaagcgcgcg cccgggatgt gacgcgcagt accgccgtgc ggcagtcggc gtcaggctgg
     4501 gcgatcacga aaaagtcgcg caactccgca gtcggcggcg agaagagcga gacatcgcgg
     4561 tagatgccgc tgaaacggaa catgtcctga tcctcgagat aagagccgtc cgaccagcgg
     4621 tacacctcca ccgccatcac attcccgccg ggcttgagat accgtgtgag gttgaattcc
     4681 gcaggcagtt tgctgtcttc gctgtatccg acatactgac cgttcaccca gaggtagaag
     4741 gcggaataga ccccgtcgaa gcgcacgaac acgggtcgtc ccgcccatgt ctccggcacg
     4801 gtgaaccggg tgcggtaggc gctgaccgga ttgtaggccg tgccgatctt gggtggggta
     4861 cgggcatgcg ggtacttgac attcgtatag atcgggatgc cgaacccccg catctccaca
     4921 caactcggaa catccagcgt gaaccagcgc gcatcatcaa actccggttc gtggaacccg
     4981 gccgggcggt catcagggct cccgctccac tgataacgcc acatgccgtt gagcgacacg
     5041 cggaacgggg tctgcggctc ctccggcgtc agtgcggcgc gcgcgtcggc gagcggaaag
     5101 ccggcggccc gcgccggttc acggttcacg gcgttgacct gctcattctc ccactcgcgc
     5161 gcgtccgcac ccctcccgca caggcacagc ccccagacga caaccacgac ccacccgatc
     5221 acgttcatac cgtattctcc tgaaaaggtt ttcctaatgc cgtcattata cccgatttcc
     5281 gtttatgcgg ccaagacgca atgcgatttc cctggtttcc ctgaaacctc aaaaaagacg
     5341 ccggaaaccc gagcgaaccc gcatgggtaa gggctagccg taccgcctat gggatgttcg
     5401 ggaccctttc taacacgttt cttaccgaaa caacgcgtta gcctaacaag aatagggttc
     5461 attaccttct atcacgttgg acgcgaatat cgggagagcg ttgtaaaaga ggagggttga
     5521 gatgaggagt gttctgttta cacaaaaaat taaagttctg atggctgttt gcctggttat
     5581 cttgggaaca acgggcgcaa cgtgtgccga ggcgtttccc gagatgtacc ccaagtacgt
     5641 tttccttttt atcggggacg gcatgggcgc gacacatgtg cgggccacgg agtgctatct
     5701 tagagacgcc cagatccaaa gaggggttgc cgccggaatg atgaaggggc tgatcatgct
     5761 ggctttgccg gtgcagggca gggcgacgac ctttgccaaa aacaaagcga cgaccgactc
     5821 ggcggcttcg gccacggcct tgtcttcagg ctacaaggcc gataacggcg tactgaacat
     5881 cgatcttgaa accgagcgca agtttgatgt catggcgcaa tttgtgaagc gtcagggcat
     5941 gaagatgggc gtcatttcaa gtgccgcgcc gaaccatgcc acgccggccg gtttttacgc
     6001 ccatgcgaaa aatcgaaaca tgtacccgga gatcgccatg cagttggcgg tgtgcggtct
     6061 tgattatgtg ggcggcccgc ccatgctcgg tgtcgaggat acggatgcca aggccaagaa
     6121 ggccgccgca aacaacgggc acacgattgt cgaagaccgc aagagcttcg acgccctgaa
     6181 acccggtgtg aaatgcgttt gggtcaggag cgacatgccg ctggtcatcg acggcaagca
     6241 ggagatcccg cttgcggatc atgtgagcaa ggcgatcgag ttgctggcgg gaccggaagg
     6301 ttttttcatg atggtcgaag gcgcggccat cgattgggcc agccattcca atgacggtgc
     6361 cgccatggtg caggagctgc tggagttcga ccgtgccata gccagggcag tcgagtttta
     6421 ccgggcgcgg cccgacgaag tcttgattgt tgtgacagcg gatcatgaat gcggcgggct
     6481 gtcgctgatc gaggggccgc tcacggctcc cggcatgagc aagattctgg acggccaaaa
     6541 aaagtcgcgg aacgcctccg ccgaaatgtt taagaagttc gagaaggaaa agatcacgtt
     6601 tgacgaggcg ttgccgcaga tgaaggcgtt tttcggcata caacacctga gcgacgagga
     6661 gctggcgaag tgccgtgaaa tcttcgacaa gggcggcgat gagcagggca aacatgcgta
     6721 tggcgatgag cgcagaatca gcctgttctg gtcgcatctc gtcagtgccc gggcggggtt
     6781 catttggggg agcttgaacc ataccgacga tcccgtcccg gtttatgcgg tcggtgtgca
     6841 ggcggcgcgc tttgccggca gcagtgacaa cgcgctgatc ggccgctacc tccgcgatct
     6901 tatcagcgga aagtaaagaa cgaaaaagct atcctatccg gtcgcgatac ggtaccctgt
     6961 tcacgcttga taaatattcg cgtgtaagag ggaagatcca tggctaagag agttcgcgac
     7021 gcctggggaa gccggattgg cgtcgtccta gcggcggccg gcagtgcagt cggtctcggc
     7081 aact
//
LOCUS       DAOSRB010000149         7000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_9995_length_7000_cov_7.997984, whole genome shotgun sequence.
ACCESSION   DAOSRB010000149 DAOSRB010000000
VERSION     DAOSRB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9995_length_7000_cov_7.997984"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..2957
                     /locus_tag="PLZ74_08230"
     CDS             219..2957
                     /locus_tag="PLZ74_08230"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOR98349.1"
                     /translation="MLKRVCGMMAVLTAGTVCAGPAGFWRFEEGKSGETAGSVASAAN
                     AQVLAGKAGVFEKGTPPVFDAEVPHKVLWDGATATLAAPTNGVSLRFRAGEVTGNGSP
                     VGGEVVVSGKEAAVKSASFTMEAFVKVARLTTRHALVVSKRRNGQTGASWALSVTPGG
                     TLSARFDTQPGAGSGNGFNQCFGASSSLNDGQWHHVALTFEEASRKVGLYVDYQLCGK
                     GITKGTLVYDDSDLVIGRGLDGWLDEVRLSPEALHPEQFLRPERFFSEMKLRVAYVPL
                     LDQTHSRVQSALKPDLKKLGTLIPKSVPEIETTMWSLGCETLDRNLADWDAYREYLVP
                     LGIKRIRLQGGWNRTEKVKGVYNFAWLDHIVDDAISLGLEVCLETSYNNRLYEPNGAT
                     GPGGHLPSGEETLAAWDKWVEAMARRYSAKGVNEWMMYNEPNLKRDFNTPERIVENNI
                     RTAEIIKRINPKAKVGAFVLAGLGVESMETMLTMIKERGKLDLFHWAVYHGYSGNPDR
                     LCERMGQFNAMLKCVAPSIRPWQGEAGCASEEVQYALSGIDWTEYSHAKWNARRMLCD
                     VGCGIESSVFTISDLSYHKDFISRYGLLKTNPDNSLIKVKSAYYTVQNVVTLFNDALT
                     LTPEREVTVTGTERRISKFAFRDKKSGLDVIALWDGTEIPGNGSEILPVQVAVKGGHF
                     KDPVWVDLITGVVYAIVPEQMKAEGGTVTFTNIPLYDGPAAILDRSLVNYEPVKVVRK
                     KKAKAVKSAKPAQGQKPAKAGASPVVAHLLPGTQQPAPAVLILGMKDADAGELITWLN
                     GQDVHAFVLAKDEKPVEGSAEKVKGALAAIRGRAAEWQVKTDAVGVMGQGATGAAAAT
                     VAGGHDADFVILIDGADAQASKMAVGAKRVPLFVGKKDAWQTPLAAWLEKFKGKVF"
     gene            3077..6319
                     /locus_tag="PLZ74_08235"
     CDS             3077..6319
                     /locus_tag="PLZ74_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR98350.1"
                     /translation="MIKGFQEVSAQRRKIDGWLSFGFGLLAAGVYGLTVSRGVYPGES
                     ARLMAICSGIETAELPLHPVWGTLAGAVSRLSFFTLPVRMNLLSVVCGVIAVMLLYRV
                     MAFLIRNTLYEEYSIEYAPRVATLAASVTVLAFLFSVPMWKASTRLHYQSFDILLALV
                     SAYLLVCYANSGWRLFLVVFAVLQGIGVVESVLFIPLAPVFLVFLIYVLWKREALTLT
                     RVAWIGVLAVAGLSLYYVAARRFLDAQGSALGFSSVMDVLVQVWKNQIRQLRSGLPRV
                     GWLVLILMSVVPWVASGLTAFRALNNERSWSQYILHITLSVLVVLGLANAEISPWEII
                     KPTGRLPVCSYLMLSMTAGYLVAYWYLLLVVKRPRQGHEVKAITQQTGDWLGLIFTYP
                     LALFVVLAALINAFECGGQSGAFADRCAREVLDRLDGRTWFVTDGTLDPHLQILARER
                     GQELNLLCLQKDMSPHYLQFLAKMVEEKQMFEPTERQRMKNTLELGVLPFIQDWFAMD
                     REIEKKVAVFGVPDFWYTAGITPVPEFFFFSGCRDIRTLQDRPLLEEYKAFWKEMDVP
                     LARDKKGSDDPTVRLRAHLRRHMGFVANNLGVLLEDLGKDAEAFAVYTIVHDQIDPDN
                     VSAMFNRFEMARRGVSAANPFKESIERELKDFIGNLKQKYPLWSLSRYYGYVRSPEIF
                     ARLGWGWALSGQTGAALAGVTRAINLLPTDQRVNAMQAMAAVYALSEDRNKTEAVYQD
                     IIRSDPKNRAAMLGLARLAVRDGALDKAKQWLEKAAQSDDRKGSLGVEWAVIHLMNND
                     IGRARLALQELTDLQPKNLQAWAMLAMVQLQQKEDADVEKVILPKMESIAGTPDDYFI
                     QITRAQVAMRKGKNFQRVAREGFIRASMLRPDIPGVKDMILQLDIAMNDQEAAELHAR
                     QVLRVNRKHALANYVMGSLRLQEGAYGEAEDFLRRSVEAKPSAAALNDLAEVLRRIRR
                     FDDAEAQIRAAIKMNAELYVAWETLGAILLDAGKDLDEAERAVNQAISLYADDLRVKI
                     TLARILLKKGEIERARETIRLLRNRQGELSRFDQEVLARLVEEASGRKAR"
     gene            complement(6363..>7000)
                     /locus_tag="PLZ74_08240"
     CDS             complement(6363..>7000)
                     /locus_tag="PLZ74_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98351.1"
                     /translation="ILGAIVLTAGVAALIVAGFYGTAAGKELMAQGKVVLDPVALLAN
                     LFGDKAGGVLAFLLAICAFPSCCFSALIAANSIKTTLPKINPWISCGIGCVIAEILAM
                     TGVALKLPVIFGFFGASFGPICGAMVAEYFINKGRWSGPRAGFNPAGWIAWLVGFVIG
                     AQPQLVAWGLPLGKPLPLSLILAFFAGAVIYAQLAKIQTPVLPYPQASPER"
BASE COUNT         1320 a         1883 c         2450 g         1347 t
ORIGIN      
        1 acgcggcggt tcctgttgga gccgtatgcg gtttcgctgc tgctggtcga gccgatttca
       61 aaatagacgg ggttcctcct tcttgggcca caccagtctg tgcgcaaaaa ggacgttctt
      121 ttttgcgcaa ccgtgtgtat tcaaatgacc gcgattcgtg tacagtgttg gcaacaggaa
      181 tccggattca ttgtcggcga ggtaaaaagg gggcggatat gttgaaaagg gtatgcggaa
      241 tgatggccgt gttgacggcg ggcacggtct gtgccgggcc tgcgggcttc tggcggttcg
      301 aggaggggaa gtccggtgag acagcgggtt cggtggcgtc ggcggcgaac gcgcaggtgt
      361 tagcggggaa ggcgggggtt ttcgaaaagg ggacgccgcc ggtttttgat gcggaagtgc
      421 cgcataaggt gctttgggac ggggcaacgg ctacattggc cgcgccgacc aacggagtgt
      481 ccctgcggtt ccgcgcgggg gaggtgacgg gaaacggttc gccggttggc ggggaagtcg
      541 tggtatccgg caaagaggcg gcggtgaagt ccgccagttt cacgatggag gctttcgtaa
      601 aggtggcccg gctgaccacg cgccacgcgc tggtcgtgag caagcggcgt aacgggcaga
      661 cgggcgcgag ttgggcgctc tcggtcacgc ccggcggcac gttgagtgcg cgcttcgaca
      721 cgcagccggg tgccgggagc ggaaacggct tcaaccagtg cttcggcgcc tcgagttcgt
      781 tgaacgacgg gcagtggcac catgtggcgt tgaccttcga ggaggcatcg cggaaggtcg
      841 gtttatatgt ggactatcag ctgtgcggca aaggcatcac gaagggaacg ctggtgtacg
      901 acgacagcga tctggtgatc ggccgcgggc tggacgggtg gctggacgaa gtgaggttgt
      961 cgcccgaagc gctgcaccct gaacagttcc tgcggcccga gcgctttttc tcggagatga
     1021 agctacgggt cgcctacgtc ccgctgctgg atcagacgca ctcgcgcgta cagtcggcgc
     1081 taaagcccga cttgaagaaa ctcggcacgc tgatccccaa gagcgttccg gagatcgaga
     1141 cgacgatgtg gtcgctgggc tgcgagacgc tggaccggaa tttggcggac tgggacgcct
     1201 accgcgagta tctggtgccg ttgggcatca agcgcatccg gctgcagggc ggatggaacc
     1261 ggaccgaaaa agtgaagggc gtctataatt tcgcatggct cgaccacatt gtggatgatg
     1321 cgatcagcct ggggctggag gtatgcctgg agacctccta caacaaccgg ctctacgagc
     1381 ccaacggcgc gacggggccg ggcgggcatc tgccttccgg cgaggagacg ctggcggcgt
     1441 gggacaaatg ggtcgaggcc atggcgcgcc gttattcggc caagggcgtg aacgagtgga
     1501 tgatgtataa cgagccgaat ctgaaacggg atttcaacac gccggagcgg atcgtcgaga
     1561 acaacatccg tacggctgag atcatcaagc ggatcaatcc gaaggccaag gtgggcgcct
     1621 tcgtgctggc cgggctgggt gtggagtcga tggaaacgat gctgacgatg atcaaggagc
     1681 gcggcaagtt ggatctgttc cattgggccg tctatcacgg gtacagcggc aacccggacc
     1741 gcttgtgcga acggatgggg cagttcaacg cgatgctgaa gtgcgtcgcg ccgtcgatca
     1801 ggccgtggca aggcgaggcg ggctgcgcct cggaggaggt gcagtacgcg ctttccggca
     1861 ttgactggac cgagtattcc cacgccaagt ggaacgcgcg gcggatgctg tgcgatgtcg
     1921 ggtgcggcat cgagtcgtcc gtgttcacca tctcggacct ctcctaccac aaggatttca
     1981 tctcacggta cggactgctc aagacaaacc ctgacaactc tctcatcaag gtgaagtcgg
     2041 cctactacac ggtgcagaac gtggtcacgc tcttcaacga tgcgctgacg ctcacgccgg
     2101 aacgtgaggt gacggtgacg ggcacggagc ggcggatctc gaaattcgcg ttccgcgata
     2161 agaagagcgg tctggacgtg atcgcgcttt gggacggcac cgagattccc ggcaacggct
     2221 cagagatcct tcccgtccaa gtcgcggtca agggcggtca cttcaaggat ccggtgtggg
     2281 tggacctgat cacaggcgtg gtgtatgcga tcgtgccgga acagatgaag gcggagggtg
     2341 gcacggtgac gttcacgaac attcccctgt acgacgggcc agcggcgatt ctggaccgga
     2401 gtctggtgaa ctatgagccg gtgaaggtcg tgcggaagaa aaaagcgaaa gccgtaaaga
     2461 gcgccaagcc ggcgcagggg cagaagccgg ccaaggccgg cgcctcgcct gtggtggcgc
     2521 atctgcttcc gggaacgcag cagcccgcgc ccgcggtact gatcctcggc atgaaggacg
     2581 cggatgcggg tgagctgatc acgtggctga acgggcagga cgtccacgcc tttgtgctgg
     2641 cgaaggacga aaaaccggtg gaggggagtg ccgagaaggt gaagggcgcg ctggcggcga
     2701 tccgcggccg cgcggcggag tggcaggtga agaccgacgc cgtgggtgtg atggggcagg
     2761 gagcaaccgg tgcggctgcg gccaccgtag cggggggcca tgatgcggat tttgtgattc
     2821 tcatcgacgg ggcggatgcg caggcttcga aaatggctgt cggggcgaaa cgcgtgccgc
     2881 tttttgtcgg caagaaagat gcgtggcaga caccgctcgc agcgtggctg gagaagttta
     2941 aaggcaaagt tttctgagcc gattgccgga cttgcggaga gggggtcaaa ccgttatact
     3001 tttctgtcat gatacagatc agtcggtttg cattaagcca aggggtcgtt tttcttcaaa
     3061 gcaaggttga tgagccatga ttaaaggttt ccaagaggtg tctgcgcaac ggcgtaaaat
     3121 cgacggttgg ttgtcgttcg gcttcgggtt gctggcggcg ggcgtttacg ggttgaccgt
     3181 gtcgcgcgga gtgtaccccg gagagtccgc gcgcctgatg gcgatatgca gcgggatcga
     3241 gacggcggag ctgcccttgc atccggtttg gggaacgctg gccggggcag tcagccgatt
     3301 gtcttttttc acgctgccgg tgcggatgaa tctgctcagc gttgtgtgcg gggtcatcgc
     3361 cgtgatgctg ctctaccggg tcatggcgtt tctgatccga aacaccctgt acgaggagta
     3421 ctcgatcgaa tacgccccgc gggtcgccac gttggccgca tcggtcacgg ttctggcctt
     3481 tctcttctcg gtgccgatgt ggaaagcctc gacgcgcctg cattaccagt cttttgacat
     3541 cctgctcgcg cttgtttccg cgtatctgct cgtgtgctat gcgaactcgg gatggcggtt
     3601 gtttttggtg gtgttcgcgg tgctgcaggg gatcggggtc gtggagtcgg tgctcttcat
     3661 tccgctcgct ccggtgtttc tggtattcct gatttatgtg ctgtggaaac gtgaggcgtt
     3721 gacgctcacg cgggtcgcgt ggatcggcgt gctggcggtg gcgggtctct ccctctacta
     3781 tgttgcggcc cggcggtttt tggatgcgca aggcagtgcg ctggggttct ccagtgtcat
     3841 ggatgtgctg gtgcaggtct ggaaaaatca gatccgccag cttcgttccg ggttgccgcg
     3901 cgtgggatgg ctggtgctga tcctcatgag cgtggtgccg tgggtggcca gcgggctgac
     3961 ggccttccgc gcgctcaaca atgagcgttc ctggagccag tatatcctgc acatcacgtt
     4021 gagtgtgctg gtggtgctgg gtctggccaa tgccgagatt tcgccgtggg agatcatcaa
     4081 gccgaccgga cgcctcccgg tttgctctta cctgatgctg tccatgacgg ccgggtatct
     4141 ggtggcctac tggtatctgc ttctggttgt gaaacgtccg cgtcaggggc atgaggtgaa
     4201 ggcgatcacc cagcagaccg gggattggct cgggctgatc ttcacctacc cgctcgcgct
     4261 gtttgtggtg ctggcggcgc tgatcaacgc cttcgagtgc ggcggccaga gcggcgcgtt
     4321 cgctgaccgc tgtgcgcggg aggtccttga ccggctcgac gggcgcacct ggttcgtgac
     4381 agatggcacg ctggatccgc atctgcagat tctggcgcgc gaacgcgggc aggagctgaa
     4441 tctgctttgt ctgcagaaag acatgagccc ccattatctc cagttcctcg ccaagatggt
     4501 cgaagagaaa cagatgttcg agccgaccga acggcagcgg atgaagaaca cgttggagct
     4561 tggcgtgctg ccgttcattc aggactggtt cgcgatggac cgcgagattg agaagaaggt
     4621 ggcggttttc ggcgtgccgg atttctggta caccgccggg atcaccccgg tgccggagtt
     4681 tttctttttc agtggatgcc gtgacatccg gactctccaa gaccggccgt tgctggagga
     4741 gtacaaggcg ttctggaagg agatggacgt cccgctggcc cgcgacaaaa aggggagcga
     4801 cgacccgacg gtgcgcttgc gcgctcacct gcggcggcat atgggttttg tggcgaacaa
     4861 cctcggtgtg ctgcttgagg atctcgggaa ggatgcggag gcctttgccg tttacacgat
     4921 cgtccatgac cagatcgatc ccgacaacgt gtcggccatg ttcaaccgct ttgagatggc
     4981 gcgccggggg gtcagcgcgg ccaatccgtt caaagagtcc atcgagcgcg aactcaagga
     5041 tttcatcggc aatctcaaac agaaatatcc gctgtggtcc ctgtcgcgtt attacgggta
     5101 tgtgcggtcg ccggagatct tcgcgcgcct cggatggggt tgggcgctgt ccggccagac
     5161 cggagccgca ttggcggggg tgacgcgcgc catcaatctg ttgcccacgg atcagcgcgt
     5221 caacgccatg caggccatgg cggcggtgta cgcgctgtcg gaggatcgca acaaaaccga
     5281 ggcggtgtat caggacatca tccgcagcga cccgaaaaac cgcgccgcca tgctcgggct
     5341 tgcgcgtctt gcggtgcggg atggcgcgtt ggataaagct aaacagtggt tggaaaaggc
     5401 ggctcaatcg gatgaccgga aaggctcgct gggtgtcgag tgggcggtca ttcatctgat
     5461 gaacaacgat atcggccggg cgcggctcgc gttgcaggaa ctcacggacc tccagcccaa
     5521 gaacctgcag gcgtgggcga tgttggccat ggttcagttg cagcagaaag aggatgcgga
     5581 tgtggagaag gtgattctcc cgaagatgga gtcgatcgcc ggaaccccgg acgattattt
     5641 cattcagatc acgcgcgcgc aggtcgcgat gcgcaagggc aagaatttcc agcgcgtggc
     5701 ccgcgaagga ttcatccggg cctccatgct gcggccggat atccccggcg tcaaggatat
     5761 gattctgcaa ctggatatcg cgatgaacga tcaggaggcg gcggaactgc atgcccgtca
     5821 ggtgttgcgc gtcaaccgca aacacgcgct cgcgaattat gtgatgggat cgctgcgtct
     5881 gcaagaggga gcgtacggcg aggcggagga ttttctgcgc cgcagtgtgg aagccaagcc
     5941 gagtgccgcg gcgctgaacg atctggcaga ggtgctgcgc cggatccgtc ggtttgacga
     6001 cgccgaggcg cagatccggg ccgccatcaa gatgaacgcc gagctgtacg tggcgtggga
     6061 gacgctggga gcgattctgc tggatgcagg caaggatctc gatgaggcgg aacgcgcggt
     6121 caaccaggcg atcagcctgt acgcggatga cctccgcgtc aaaatcacgc tggcccgcat
     6181 cctgctcaag aagggggaga tcgaacgggc gcgcgaaacg atccggctac tccggaaccg
     6241 gcagggcgaa ctctcccggt tcgatcagga ggtgttggcc cgtctcgtgg aagaggcctc
     6301 cggacgcaaa gcccggtagg ctgatgcgtc cgctttttcg ggaacggtga gggtaaaagc
     6361 gctcaccgtt caggtgaggc ctgcggataa ggcagaaccg gcgtctggat tttcgccagc
     6421 tgcgcgtaga tcaccgctcc agcgaagaag gccagaatca ggctcagcgg caacggtttg
     6481 ccgaggggca gaccccaggc gacaagctgc ggttgcgcgc cgattacgaa gccgaccagc
     6541 caagcgatcc agcctgccgg gttgaagccc gcgcgcggcc cgctccagcg gcccttgttg
     6601 atgaagtact cggcgaccat cgcgccgcag atcggtccga aggaagcgcc gaagaaaccg
     6661 aagatcaccg gcagcttgag cgccacgccg gtcatcgcca gaatctcggc gatcacgcat
     6721 ccgatgccgc aggagatcca ggggttgatc ttgggcagcg tggtcttgat ggagttggcc
     6781 gcgatcagcg cggagaaaca gcagctcggg aaggcgcaga tggccagcag gaaggccaac
     6841 acaccgcccg ccttatcgcc gaacaggttg gccagaagcg ccaccggatc cagcaccacc
     6901 ttgccctgcg ccatcagctc tttgccagcc gccgtgccgt agaaaccggc gacgatcaga
     6961 gccgcgacgc cagcggtcag gacgatcgct ccgaggatgc
//
LOCUS       DAOSRB010000150         6996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_10003_length_6996_cov_6.388129, whole genome shotgun sequence.
ACCESSION   DAOSRB010000150 DAOSRB010000000
VERSION     DAOSRB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10003_length_6996_cov_6.388129"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLZ74_08245"
     CDS             complement(<1..593)
                     /locus_tag="PLZ74_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98352.1"
                     /translation="MRSNSVKQLKTVRAAQARSVADAALNAGGGVLRLAPNWVPRSFC
                     YPGRRLKLHPDDLYAFGLNRGGIDERWFGSTTEAANEGRLPDEGLSYVVFENKRVLLR
                     DVVAELGATIVGAKIWKKYRRWPVYSKFFDNLGPLPHHLHQDDKLAKLVGQEGKPESY
                     YFPPQHNNLGARFPYTFFGLVPGTTKADIVRCLERWN"
     gene            complement(634..3309)
                     /locus_tag="PLZ74_08250"
     CDS             complement(634..3309)
                     /locus_tag="PLZ74_08250"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR98353.1"
                     /translation="MNKTFWVMVTGLAACLVACAELDKAILLDQDKLIEVLGKAETSD
                     NDRVTACQNLGWCGTRKAIEPLAGLLGDEKPFLRHAARYGLEMIPDPEVEVALCAAAE
                     RLTGPALVGVLQSLGNLRRCQSRRSWVSALLGGSRYAQAVEVLRARMTDPDKQVAGAA
                     IQALGKLATSEAVEALAPHLGACPAVARAYLEAAGRIKAKDPDAAAPLYAALRTAGAA
                     VPDTVRLAALRGEIITSGRRGLELWRASIASSDPDTVDVALRAVLDTPSCAKATVAFA
                     GALAEVPAVRIRLAAVLGRRGDKASVPALAALANGTGTECPADRLAAAQALAMLNDAS
                     ALPPLLALARHEQGTIANGAKDAIMGFAGKAADDAVLSMMTEADASVRLAGIDMAMRR
                     RMAVAVPTLARLTRDRDPAILNAAIKGVGELGSFEEIPVLLAAIAGNPQNENASRALV
                     TLCSRYARPRGGVVKVESAVYGNFENNLTKDVTENVQKLADAGSITIQASGRLCRWDG
                     FGEDPAPGKPKVLRMVYLFDGVKKSVQIRENDSAHLSGETLLPVAMDPLRAAYDAATG
                     AEKVVLFRVLAGLANEQGLAVARAAAVQNKDEALREAAIRALAEWRTPEALEDAASFA
                     QQAPSDRLRILALRGFVRQLEQSFTIPFERQIRLLEQAGSWAVREEDKALLKAALAVT
                     RRMQAEQGFVPMFNGSDMKQWKGGDGWWQVKEGVLQAQSSEEKPCKKNSHLIWTGGEP
                     GDFELRAEFRLSPGANSGIQIRARNQEFGDSGYQADMNGGGNYVGYLYHPKQHLVGER
                     GAKVVIAADGKKTVERFADSKELQDKVFKGDDWNEYSIICKGPSITLFVNGMKTCEFE
                     DHRPDTPRKGHITLQMHAGPPMKIQYRNLRIKEFK"
     gene            complement(3349..4689)
                     /locus_tag="PLZ74_08255"
     CDS             complement(3349..4689)
                     /locus_tag="PLZ74_08255"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98354.1"
                     /translation="MSKSEHGGFRRDGCGTLGRRAFLKGTLTAAGAVAMPTLIPASAL
                     GKNGAVAPSNRIVMAGIGLGGRGTADLRSWILPDPEAQFVAICDVQRSRRAAIKAMTD
                     KHYGNKDCKMYVEMMEVLERPDIDAILTATSDRLHATVSVRAMRAGKDVYTEKPASMT
                     IAEGQAVVQTARQYGRVYQAGMQRLSEANFIVCNELLRLGRLGEVKTVYAHLAPWGNV
                     NMSREWLAAEPLPDPDELDWDRWLGPCPWRPFNKHYVHGGWHAQHDFYTSVIGEWGSH
                     TFAQCHDALGLQDTSPVHYPYVSNPTADGMEMTFANGVRMIQKQEGWRGTCGVRYVGS
                     EGWCACADGYARPEVSNPALLADYNKILQDYITRTGHVVGPMRDFFQCIRRRTVTVAN
                     PVLMHRSMSTVHCANIAQWLKRDLTWDPVREEFVNDEQANRFRSRAAREGWQIC"
     gene            4987..6078
                     /locus_tag="PLZ74_08260"
     CDS             4987..6078
                     /locus_tag="PLZ74_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOR98355.1"
                     /translation="MYVVRCGDYGASLPVALNRLLDLTGWLRDGSLAGKRVLLKPNLL
                     TERSPEQAVTTHPELVRQMLRALKPAGARLAVGDSPASTANLENVWRATGMAAVCAEE
                     EVPLISFEQAGVLAFERNGFAFSVARPVLEADLIVNLPKVKSHSLTLLTAAVKNLYGA
                     IPGYAKTTLHRLHPRPATFGALLRTLWDVLPPVWNLADGIVGMEGQGPANGRPVQLGF
                     LAASRDPFALDRALCDLLHIDARRVPFLPPGDAVRVTGDPVSIATFATPSGGHLLALA
                     PPWLVRCAGRCVWVRPAFSARACIRCGLCVKACPVHALSLAPEARTPTLDTARCISCS
                     CCHEVCPANAIRMSQSRLLRLLGVFKGLD"
     gene            complement(6097..6567)
                     /locus_tag="PLZ74_08265"
     CDS             complement(6097..6567)
                     /locus_tag="PLZ74_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOR98356.1"
                     /translation="MVRFKEIIGHSTVCLNLDETDGPQVVARLIQAAVDEGRLPAERR
                     DEVVQTVLKRERGASTAMPDGIALPHGRTDCVRDILCVIGIHPRGIEFGAPDLEPTHI
                     FVLLLVPLTVGCNHISFLANLSRRLMEQSVRDNLVRALSREAVLRTLLEEGTES"
     gene            complement(6586..>6996)
                     /locus_tag="PLZ74_08270"
     CDS             complement(6586..>6996)
                     /locus_tag="PLZ74_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOR98357.1"
                     /translation="REAVDWGVDFVVGFDDDGLMAEGATENFGIVTRAGELLFPRVET
                     VLDGTTMLRVMQLAEPLAAEGALRAIGFRDITEADVRDAAEMLVVGTTINVVSVREFE
                     GVPIADGRPGKIGQTLNDLLTRDIETNAALRTVY"
BASE COUNT         1185 a         2453 c         2067 g         1291 t
ORIGIN      
        1 ttgttccagc gctcgagaca gcggacgatg tccgccttgg tcgtgccggg caccagaccg
       61 aagaaggtgt aagggaaacg cgcgcccaag ttgttgtgct gcggcgggaa gtagtaggat
      121 tccggcttgc cctcttgccc caccaacttg gcgagcttgt cgtcctgatg caggtggtgc
      181 ggcagcggcc cgaggttgtc gaagaacttg ctgtagaccg gccagcgccg gtacttcttc
      241 cagatcttcg cgccgacgat cgtcgcgccc agttcggcca ccacatcgcg caacagcacg
      301 cgcttgttct cgaagaccac atagctcagg ccctcatccg gcagacgccc ctcgttcgcc
      361 gcctcggtgg tcgagccgaa ccagcgctcg tcgataccgc cgcggttaag cccgaaggcg
      421 tagaggtcat ccgggtgcag cttcaacctg cgtccgggat aacagaacga gcgcggcacc
      481 cagttcggcg ccagccgcag cacgccgccg cccgcgttca acgcggcgtc cgccacagag
      541 cgcgcctgag cagcacgcac ggtcttcaac tgttttacag aattgcttct catgtgggac
      601 ccctttcttt tcggatcggt tacgacacct tgtttacttg aactccttga tgcgcaaatt
      661 gcggtactgg atcttcatgg gcggaccagc gtgcatctgc agcgtgatgt ggcccttgcg
      721 gggcgtgtcc ggccggtgat cctcgaactc gcaggtcttc atgccgttga cgaaaagcgt
      781 gatggacggc cccttgcaga tgatgctgta ctcgttccag tcatcccctt tgaagacctt
      841 gtcttgcagt tctttgctgt ccgcaaaacg ctcgaccgtt ttcttgccgt ctgcggcgat
      901 cacgactttg gcaccgcgct cgccgaccag atgctgcttg ggatggtaga ggtagccgac
      961 atagtttcct ccgccgttca tgtccgcctg atacccgctg tcgccgaact cctgattccg
     1021 cgcgcggatc tgaataccgg aattggcgcc gggactgagc cggaactcgg cccgcagctc
     1081 gaaatcgccc ggctcgccac cggtccaaat cagatggctg ttcttcttac aaggcttctc
     1141 ctccgaactc tgcgcctgca agaccccctc tttcacctgc caccagccgt cgccgccctt
     1201 ccactgcttc atgtccgaac cgttgaacat cgggacgaac ccttgttcgg cctgcatgcg
     1261 ccgcgtgaca gccagcgccg ccttgagcaa ggccttatcc tcttcgcgca cggcccaact
     1321 cccggcctgt tccaacagac ggatctgacg ttcgaacgga atcgtgaagc tttgttcgag
     1381 ctgccgcaca aacccgcgca atgcgagaat cctcaaccgg tcggagggtg cctgttgcgc
     1441 gaacgaggcg gcatcctcca gcgcctcggg tgtacgccac tccgcgaggg cgcggatcgc
     1501 cgcttcacgc agcgcctcat ccttgttttg cacggccgct gcacgggcca cggcaagacc
     1561 ctgttcgttc gccagaccgg ccaagacgcg gaacagaacg accttttcgg caccggtggc
     1621 cgcgtcgtag gcggcccgga gcggatccat ggccacgggc agcaaggtct cgccgctcag
     1681 atgggccgag tcattctccc ggatctgcac gctctttttc acaccgtcaa acaggtagac
     1741 catccggagc accttgggtt tgccgggcgc aggatcctca ccgaaaccgt cccaacggca
     1801 gaggcggccc gaggcctgaa tcgtgatgga tccggcatcc gcgagtttct gcacattctc
     1861 cgtgacatcc ttggtgaggt tgttctcaaa attcccgtag accgcactct caaccttgac
     1921 tacgccgccg cgcgggcggg catagcgcga gcagagcgtg acgagcgcac ggctcgcatt
     1981 ctcgttctgc gggttaccgg caatggccgc caaaagcacc ggaatctcct cgaaggagcc
     2041 cagctcgccc actcccttga tggcggcgtt cagaatggcg gggtcccgat cccgtgtgag
     2101 ccgcgccagt gtgggcacgg ccacggccat gcgccgacgc atcgccatat cgattccggc
     2161 gagacgcacc gacgcatccg cctcggtcat catcgagagc accgcgtcat ccgcagcctt
     2221 ccccgcaaaa cccatgatcg catccttggc accgttggcg atcgttccct gttcgtgccg
     2281 ggccaacgcc agcaacggag gcagggcgga agcatcattt agcatagcga gcgcttgggc
     2341 cgcggcgaga cggtcggccg gacattcggt ccccgtaccg ttggccaagg ccgccagagc
     2401 gggcacggag gccttgtctc cgcggcgacc gagcaccgct gccaaccgga tccgcaccgc
     2461 aggcacctcc gccagcgccc cggcgaacgc cacggtggcc ttggcgcaag acggggtgtc
     2521 gagcaccgcg cgcaacgcga catccaccgt gtccggatcc gaactcgcga tcgacgcgcg
     2581 ccacagttcc agtccgcggc gaccggaggt gatgatctcg ccccgcaacg cggccagacg
     2641 caccgtgtcc ggaaccgctg cacccgccgt gcgcagcgcc gcgtagagag gcgcggccgc
     2701 atccggatcc ttagccttta tgcggccggc ggcctccaga taggcccgcg ccacggcggg
     2761 acaggcgccg agatgcggcg cgagcgcctc gaccgcttcc gacgtggcca gtttaccgag
     2821 cgcctgaatc gcggctcccg cgacctgttt gtcggggtcc gtcatgcgcg cgcggagcac
     2881 ctcgaccgcc tgcgcgtaac gcgacccgcc cagcaacgcg gacacccaac tgcgacgcga
     2941 ctggcatcgc ctgagattcc cgagcgactg cagcacgccg accaaggccg gtccggtgag
     3001 ccgttccgcc gccgcgcaca aggcgacctc gacctccgga tcggggatca tctccagccc
     3061 gtaccgcgcc gcgtgccgca ggaacggctt ctcgtcaccc aacaacccgg ccagaggctc
     3121 gatcgccttg cgcgtaccgc accagccgag attctgacag gcggtgaccc ggtcgttatc
     3181 cgaagtctcc gccttgccca acacctcgat cagcttgtcc tgatccagca gaatcgcctt
     3241 gtccaactcc gcgcacgcca caaggcacgc ggccagaccc gtcaccatca cccaaaacgt
     3301 cttgttcatc cttgttcccc gtatccccgt tgtgaaattc gtaaggtcct agcagatctg
     3361 ccagccctcg cgcgcggcgc gggagcggaa ccgattggcc tgttcgtcgt tgacgaactc
     3421 ctcccggacc ggatcccatg tgagatcccg tttcagccac tgcgcgatgt tcgcgcagtg
     3481 cacggtgctc atcgaccggt gcatcagcac ggggttggcc accgtgaccg tgcgccgccg
     3541 gatacattgg aagaaatcgc gcatcggacc gaccacatgc cctgtgcggg taatgtagtc
     3601 ttgcaggatt ttgttgtaat cagccagcag cgcgggattg gacacctccg gccgcgcgta
     3661 accgtcggcg cacgcgcacc agccttcgct gccgacatac cgcacgccgc aggtgccgcg
     3721 ccatccctcc tgcttctgga tcatccggac cccgttcgcg aaggtcatct ccatcccgtc
     3781 ggccgtcgga tttgaaacat aggggtaatg gactggcgag gtgtcctgca agcccaaggc
     3841 gtcgtggcac tgcgcaaagg tgtgcgaacc ccactcgccg atgacgctgg tgtagaaatc
     3901 atgctgcgcg tgccacccgc catgcacgta atgcttattg aaggggcgcc acgggcaggg
     3961 gccgagccag cggtcccagt cgagttcgtc cggatccggc aacggttcgg ccgcgagcca
     4021 ctcccggctc atgttgacat ttccccaagg cgcgagatgc gcgtaaacgg tcttgacctc
     4081 acccagccgt cccagacgca gcaattcgtt acacacgatg aagttggcct cgctgagacg
     4141 ctgcatgcca gcctggtaga cacggccgta ctgccgcgcg gtctgaacca ccgcctgtcc
     4201 ttcggcgatc gtcatggagg ccggtttctc cgtgtagaca tccttgcccg cgcgcatggc
     4261 ccggaccgaa accgtggcat gcagccggtc gctggtggca gtgaggatcg catcgatgtc
     4321 gggccgttcc agcacctcca tcatctccac gtacatcttg cagtccttgt tgccgtaatg
     4381 tttgtccgtc atcgccttga tcgccgcacg gcgggaacgc tggacatcgc agatcgcgac
     4441 aaactgcgcc tccgggtccg gcaggatcca agacctgaga tccgctgtgc cgcgcccgcc
     4501 gagaccgatc cccgccatca cgatccggtt gcttggggcc accgccccgt tcttccccaa
     4561 ggccgacgcg ggaatcaacg tcggcatcgc caccgcgccc gccgcagtca gggtcccctt
     4621 caaaaacgcg cgccgcccca gtgtgccgca tccgtcacgc cggaaaccgc catgctctga
     4681 cttgctcatg cccttctctc cttttctgtt cttttcaatt aaagtcccct tttcagtata
     4741 gtcggcatcc tttccgcctt ttgatcgttg aatggtatcc gatctggtta gatttgtcga
     4801 aaactcattt ttgtgaaatc gagaacggga aagacgggcg tccgctcgtc cgggcaggca
     4861 gacgcttgcc ccttccggtc tgtggcaccg ctatcgagat cgggatcacc tcctcgtccc
     4921 cgtctccgta tccggccgct ggcctccgac accgtgccct gctatactgc gcacatggat
     4981 ccggaggtgt atgttgtacg ctgcggcgac tacggggcat ccctgcccgt ggcgttgaac
     5041 cgtttgttgg atctcacagg gtggctgcgc gatggaagcc tcgcgggcaa gcgtgtgctg
     5101 ctgaagccga atctgctcac cgaacgctcc ccggaacagg cggtcaccac ccaccccgag
     5161 ctggttcgcc agatgctccg tgcgctcaag cccgccggcg cgcgcctcgc cgtgggcgac
     5221 agcccggcga gcaccgccaa cctcgagaat gtctggcgcg ccaccggcat ggcggcagtc
     5281 tgcgccgaag aggaggtgcc cctgatctcg ttcgagcagg ccggcgtcct ggcgttcgag
     5341 cgcaacggat tcgccttctc cgtggcgcgg cccgtgctgg aggccgatct gatcgtcaac
     5401 ctgccgaaag tgaaaagcca ctcgctcacc ctgctgaccg cggcggtaaa gaatctctac
     5461 ggcgccattc cgggatacgc gaagaccacg ctgcaccgac tccatccccg gcccgcaacg
     5521 ttcggcgcgt tactccggac attatgggac gtgctgccgc cggtctggaa tctggccgac
     5581 ggaatcgtcg gcatggaagg tcagggaccg gccaacggcc gccccgtaca gttggggttt
     5641 ttggccgcgt cgcgggatcc gttcgccttg gaccgcgcgc tgtgcgatct cctgcacatc
     5701 gatgcgcggc gggtgccttt cctgccgccg ggggacgcgg tgcgcgtcac gggcgatccc
     5761 gtgagcatcg cgaccttcgc cacgccgtcg ggcggtcatc tgcttgcgct cgcgccgccg
     5821 tggctggtgc gctgcgctgg ccgctgcgtg tgggtccggc cggctttttc ggcccgcgcc
     5881 tgtatccgct gcggcctgtg cgtgaaggcc tgtccggtgc atgcgttgtc cctcgcgccg
     5941 gaagcgcgca ccccgacgct ggacacggca cgctgcatca gttgcagttg ttgccacgag
     6001 gtgtgccccg caaacgccat ccgcatgtcc cagtcgcgcc tgctgcgtct gctcggtgtg
     6061 tttaaggggc tcgactgacc gcagaaacgg gacgcgtcag gattccgtcc cttcttcgag
     6121 aagggtccgg agcaccgctt cccgcgaaag cgcccgcacc agattatcac gcaccgactg
     6181 ctccatcagc ctgcggctga gatttgcgag gaaactgatg tggttgcatc ccaccgtgag
     6241 cggcacgagc aacagcacga aaatatgggt cggctccaga tcgggcgcgc cgaactcgat
     6301 tccccggggg tggatgccga tcacgcaaag gatgtcgcgg acacagtcgg tccgcccgtg
     6361 gggcagagcg atcccgtccg gcatggcggt ggacgcgccg cgttcgcgct tgagtaccgt
     6421 ctgcaccacc tcatcccgcc gttccgcagg cagacgtccc tcgtccacgg ccgcctgaat
     6481 caaccgggcc accacttgcg gaccgtccgt ctcgtccaga ttgaggcaga ccgtgctgtg
     6541 cccgataatt tccttaaacc tcaccatgaa ccgttcccct tgccctcaat acacggtgcg
     6601 caacgccgcg ttcgtctcga tatcgcgcgt cagcaggtcg ttcagtgtct gtccgatctt
     6661 gccgggccgg ccgtccgcga tcggcacccc ctcaaactcg cgcacggaca ccacattgat
     6721 cgtggtcccc accaccagca tctccgcggc gtcgcggaca tccgcctccg tgatatcgcg
     6781 gaacccgatg gcccgtaacg caccctcggc cgcgagcggt tcagcgagtt gcatcacccg
     6841 cagcatggtg gtcccgtcca aaaccgtttc cacacgcggg aagagcaatt cgcccgcccg
     6901 ggtgacgatg ccgaagttct cggtggcccc ctccgccatc aggccgtcat cgtcgaaccc
     6961 caccacaaaa tccacgcccc agtccaccgc ctcgcg
//
LOCUS       DAOSRB010000151         6862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_10238_length_6862_cov_6.208315, whole genome shotgun sequence.
ACCESSION   DAOSRB010000151 DAOSRB010000000
VERSION     DAOSRB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10238_length_6862_cov_6.208315"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..731)
                     /locus_tag="PLZ74_08275"
     CDS             complement(237..731)
                     /locus_tag="PLZ74_08275"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOR98358.1"
                     /translation="MLSPDFHAHFMRQALREAARAEAAQEVPTGCVIIRRPSDPTISL
                     AAVSVLARAHNQTEMLCDATAHAEMIALTQASAALGDWRLLDTILYVTKEPCPMCAGA
                     IVLARVPLVVWGVSDPKRGGQSVFSILNHPGLNHRPETLAGVLETECRAALQTFFRSR
                     RAED"
     gene            825..1643
                     /locus_tag="PLZ74_08280"
     CDS             825..1643
                     /locus_tag="PLZ74_08280"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOR98359.1"
                     /translation="MTRMYTPTGDAGAAALLAFVTAAIREGAAVAVAPEHVPVLTRFA
                     GCRTHGRGAAAGRYVFRAPRPERPVFTTAERVLAGFLLIALLPLLGLIALLILVTDGP
                     PVLFRQIRFGEDGRPFVFLKFRTMCRASEWLHAHLQHRQTEPGRLFKLENDPRVTRFG
                     ALLRNTFLDEFPQLLNVVRGEMRLVGPRPLPQSDQHHYTRPEHAMRLKGRPGMTGLWQ
                     VAGRNRRTFDEMCLLDLYYLGNRSVTLDLWVLGRTLILIAQQIGLGGKAQQPGE"
     gene            complement(1572..2204)
                     /locus_tag="PLZ74_08285"
     CDS             complement(1572..2204)
                     /locus_tag="PLZ74_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98360.1"
                     /translation="MVFCFVLLLAVAVAGGVAGATPATFGAAAVSAALATWLLRADRG
                     VPRANHVTVTLDPVAPDSGMLLEGVREVLRRVHGTDRPARLRIQTRRAAADRPRLQLG
                     LTRDGDLDLIHDLRHTPLKQPGVWIADHPLPLNLSHVRCTTLRLTPAGGGRVRVRLTP
                     YYAWPSPPVWILLTAGIAAACLFDQAELLAGLLGFAAQAYLLRYQNECTS"
     gene            2306..3646
                     /locus_tag="PLZ74_08290"
     CDS             2306..3646
                     /locus_tag="PLZ74_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98361.1"
                     /translation="MHTIHIPEALNDFDAHVRRDALRKVCGETRFPEPGTNFNMHCHS
                     FFSYNAEGWSPSCVAYVCRAQGLCAAGLCDFDVLDGLEEFSDAGLSLGLRTAVHLETR
                     AYVPEFAEAEISSPGEPGVTYIMGCGFIRLPDPDTAQARTLARLRDGAQERNLALLSR
                     VNAALPAIAVDYARDVLPLTPMGVATERHIVRAYCNRARTVMSDPAERATFWSPVLKC
                     GTEAFAALDANVPKMEDRVRNALAKRGGLGYIQPTAQTFPLADDFIRWVLACHAIPTV
                     TWLDGTSDGEADCERLLDQMTAKGCEALNIIPDRNWNLSKPDEAALKRAKLDAMVNAA
                     IRRDLPINIGTEMNKGGLPFVDDLAGPVLSRYADAFTQGMHIMVGHTVLGRYADAPYI
                     GARAQSEFTDRKRRNAFYAAVGALPALSLSQAEALSSAGPDKAFAMLADAAAKK"
     gene            3655..5520
                     /locus_tag="PLZ74_08295"
     CDS             3655..5520
                     /locus_tag="PLZ74_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98362.1"
                     /translation="MRGGIICALLTVCLAATGRTVPAVMYVDDTAGSDAAEGLTPDRA
                     WKTLDRVNREPLIPGDRVLFRRGGVWRGQLVPRSGTNGAAVTYGAYGTGAKPVLQGSV
                     ARDRREEWVCLHPGIWATPVYEPEALDQLADLKDSRWSLHAEASARAHLSRREEDGRG
                     FNRVVCDAPGSARNHIQLWGAELDGFPSPVLLRLRVRSSIPFRLDEVEALLNRPPYTR
                     TRSGHVRSALIGKEWRTVEAVLYETATGSGAHLHLSLGEKIPAGAVFDFEPLGLWRLR
                     PETRHAPIPCDVGILLLDHGVEWGVKKWRPEDLVNRLDYWYDGEGQRVVLVCETNPAV
                     RYASIELALTRTIVLQANCRDVTYEGLAVRYGGAHGFGGSRTQRITIRQCDLSWIGGG
                     LQDWRKRKGGERFPVRYGNAIEFWNGAEDHVVESNRIWEIYDAALTNQGHGDDSRQMR
                     IRYRHNVIWNAEYSFEFWNRPETARSEHILFEYNTCVDAGSGWAHKQRPDPNGAHLMF
                     YLNSSATTNVVIRNNLFVGTTEVCMRMENDWRGGLSMRNNLYWPGDKPFLRWLGKTFF
                     EAGGFTRYQRELALDSNSLLAEPKFMNPAARDYRLCPDSPGARLATDGGPVGARW"
     gene            complement(5531..6634)
                     /locus_tag="PLZ74_08300"
     CDS             complement(5531..6634)
                     /locus_tag="PLZ74_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HOR98363.1"
                     /translation="MRMEKWAGAALALAAAIFAGGCSINRTSSFKWLAFPLSWRSAPS
                     DPTQTLREKAESGDPDSQYKLGFSLLYGEDMPRDAESGLVWLKRSGENGYLKAQSALG
                     LLFLKGVPDIDRDDREAAVWLEKAAAQGHVVSQSSLGALLLSQTSSVHDSEAGVVWLK
                     RAAGQGDAPAQVALGQMYRNGYVVAQSDTESVRWYRKAAEQGNTGAQTQLGIAYYRGK
                     GVLQSFDEARRLFEAAAFRDDALAMSNLGVMASRGEGMKQDAELALSWFRKAAEGGHA
                     DGQNNLGAAYYKGIGVPQDYGAALAWFRKAADQGHASAQFRLGLMLEAGQGTDPDTSE
                     AAVWIKRAADQGFPPAVNRMRERGAAPAPDPTP"
BASE COUNT         1261 a         2053 c         2236 g         1312 t
ORIGIN      
        1 atcgctatcg ggatcgggat cgatcattta accacggaac accaccgcct cacagtctca
       61 caacctcaca gcctcaccgc ctcacagtct caccgccttt gagattgcgg gctccgcccg
      121 catctcaata cggtgcggcg gggaccgcga caaaacccaa tgtttacggg gctttgctag
      181 aattttaccc gttcaactgc tccttttgag ctgaaaggtc aagtgctctt ttggggtcag
      241 tcttcagcgc ggcgactgcg gaaaaacgtt tgcagcgcgg cgcggcactc ggtttccagc
      301 actcccgcca gcgtttcggg acgatggttg agtcccgggt ggttgaggat cgagaacacc
      361 gactgaccac cgcgcttggg atcggacacg ccccaaacca caagcggcac ccgggccagc
      421 acgatggcgc cggcgcacat ggggcagggt tctttggtga cgtacaggat cgtgtcaagc
      481 aggcgccagt cgccgagcgc cgccgaagct tgggtcagcg cgatcatttc cgcatgcgcg
      541 gtggcgtcac acagcatctc tgtctgattg tgggcgcggg ccagcacgct gaccgcggcg
      601 agggaaatgg tggggtccga tggacgccgg atgattacgc agccggtcgg gacctcttgc
      661 gcggcctcgg cacgcgctgc ttcccgcaaa gcctgacgca tgaaatgggc atggaaatcg
      721 ggcgacaaca tgccgggatt gtaggcggac ggcggcgtga tgtaaaggcg ggctttccgt
      781 tcttgatgcg atcggaccga aacgggataa tacggggcat ggggatgacg cgcatgtaca
      841 caccgacggg ggatgccgga gccgccgcgc tgttggcatt tgtcacggcg gcgatccgcg
      901 agggcgcggc ggttgccgtg gccccggagc atgtgccggt cctgactcgt tttgcgggtt
      961 gccggactca cggtcgcggg gctgctgcag gaagatacgt gtttcgtgcg ccccgcccgg
     1021 agagacctgt gttcacgacc gcggaaaggg tgctggccgg gtttttgttg atcgcgctcc
     1081 tgccgctgtt gggtctgatc gctttgttga tcctcgtcac ggacggcccg ccggttctgt
     1141 tccggcagat tcgtttcggg gaggacgggc gtccattcgt tttcctgaaa ttccgcacga
     1201 tgtgtcgcgc ctccgaatgg ttgcacgccc acctgcagca caggcaaact gagccgggac
     1261 gcctattcaa actggagaat gatccgcgcg tgacccgctt cggcgcgtta ctgcgaaaca
     1321 cgtttctgga tgagtttccg caactcctga atgtggtacg gggtgagatg cggctggtcg
     1381 ggccacgtcc tctaccgcag agcgaccaac atcattacac gcgtccggag cacgccatgc
     1441 ggctcaaagg ccgccccggc atgacggggt tgtggcaagt ggcgggtcgt aaccgccgga
     1501 cgtttgacga gatgtgtctg ctggacctct actacctcgg taaccgttcc gtaacactgg
     1561 atctgtgggt gttaggacgt acactcattc tgatagcgca gcagataggc ttgggcggca
     1621 aagcccagca gcccggcgag tagttccgct tgatcgaaga gacaggcggc ggcgatgccc
     1681 gctgtcagca agatccagac cggtggggag ggccaggcgt aatacggcgt gagccggacg
     1741 cgaacccggc cgccgcccgc tggggtcaga cgcaaagttg tgcagcgcac atgggacaga
     1801 ttcagcggta acgggtgatc cgcaatccag acaccgggtt gcttcagcgg tgtgtgccgc
     1861 agatcgtgaa tcaggtcgag atccccgtca cgggtcaggc cgagctgcag acgcgggcgg
     1921 tctgctgccg cgcggcgggt ctggatgcgc aggcgggccg ggcggtcggt gccgtgcacg
     1981 cgcctcagaa cttcacgaac cccctccagc agcatgccgc tgtccggcgc caccgggtcc
     2041 agagtcacgg tgacgtggtt ggcgcggggc acaccgcgat ccgcgcgcaa cagccaagtg
     2101 gccaatgcgg ctgaaacggc tgcggccccg aatgttgcgg gcgtggcacc ggccacgccg
     2161 cctgcaaccg ccacggctag cagcaggaca aagcaaaaaa ccatgtcaca ccataatctg
     2221 cggtacgctt ttcaggcaag cggaaaacac cgtttccgcg cgtcggaatt ttctgctatg
     2281 ctttttgtcg cgaaaggaaa cggctatgca cacgattcac attcccgagg cactgaatga
     2341 ttttgacgcc catgtccggc gcgatgcgct ccgcaaggtc tgcggggaaa cgcgattccc
     2401 ggaacccggc acgaacttca acatgcactg tcactcgttc ttctcgtaca acgccgaggg
     2461 gtggtcgcct tcctgtgtgg cgtacgtctg ccgtgcccaa gggctgtgcg ctgcggggct
     2521 ttgcgatttc gatgtgttgg acgggttgga agagttttca gatgcgggct tgtctctggg
     2581 tttacgtacg gcggtgcatc tggagacccg tgcctatgtg cccgaatttg cggaggcgga
     2641 gatcagttcg cccggtgagc cgggcgtcac atacatcatg gggtgcgggt tcatccggtt
     2701 gcctgatccg gatactgcgc aggcccggac cctggcccgg ttacgcgatg gggcgcagga
     2761 acgcaatctg gcgctcctca gccgggtcaa cgccgcgctg cccgccattg cggtcgatta
     2821 tgcgcgtgac gtgctgcccc ttacgccgat gggcgtggcc acggagaggc acatcgtgcg
     2881 ggcctactgc aaccgcgccc gcacggtgat gtccgacccg gcggagcggg caacgttctg
     2941 gtcgccggtg ctgaagtgcg gcacggaagc gtttgcggca ctcgacgcga atgtgccgaa
     3001 gatggaggat cgagtgcgta acgcattggc gaaacgcgga ggtctcgggt atatccagcc
     3061 caccgcgcaa accttcccat tggcagacga cttcatccgc tgggtgctgg cctgtcatgc
     3121 gattcccacg gtgacctggc tggacggaac cagcgacggc gaggcggatt gcgagcggct
     3181 gctggatcag atgaccgcga aagggtgcga ggcgctcaac atcatacccg accgcaactg
     3241 gaatctttcg aaaccggacg aagcggccct gaaacgcgcg aagctggacg cgatggtgaa
     3301 cgccgccatc cggcgcgacc tgccgatcaa catcggcaca gagatgaata agggcggcct
     3361 gccattcgtg gatgatctgg ccggacccgt gctgagccgc tatgcggatg cttttacgca
     3421 gggtatgcat atcatggtgg ggcacaccgt tctcggccgc tacgcggatg ccccttatat
     3481 cggcgcgcgc gcgcagagcg agttcaccga ccggaaacgg cgcaatgcgt tttacgcggc
     3541 ggttggcgcc ctgcccgcac tttcgctctc tcaggccgag gccttgtcgt ccgccggacc
     3601 ggacaaggcg ttcgcgatgc tggctgatgc ggcggcgaaa aaatgaggtt ggtgatgcga
     3661 ggcgggatca tatgtgcgct gctgaccgtg tgtcttgctg ccaccggacg caccgtacct
     3721 gccgtgatgt atgtggatga cacggctgga tcagatgctg cggaaggcct gacgccggac
     3781 cgggcgtgga aaacgttgga tcgcgtcaac cgcgagccgc tgatcccggg tgaccgggtg
     3841 ctgttcagac ggggcggagt gtggcgaggt cagcttgttc cccggagcgg tacaaacggc
     3901 gcggcggtga cgtatggtgc ctatggcacg ggtgccaaac cggtgctgca aggctccgtg
     3961 gcccgggatc ggcgggagga atgggtctgc ctgcatcccg gaatctgggc cacgccggtt
     4021 tatgaaccgg aagcgttgga tcaattggcg gatctgaaag actcgcgctg gagcctgcat
     4081 gcggaagcga gtgcgcgggc gcacctttcc cgccgagaag aggatgggcg cgggttcaac
     4141 cgggtggtgt gcgacgcacc gggcagcgcg cgcaaccata tccagctctg gggggccgaa
     4201 ctggacgggt tcccctcgcc tgtcctgctg cgtctgcgtg tgcgttcctc aatcccgttc
     4261 agactggatg aggtcgaagc cttgctaaac cggccaccgt acacccgtac ccgcagcggg
     4321 catgtccggt ccgcactcat cgggaaagaa tggcggacgg tcgaagcggt gttgtacgaa
     4381 acggcgaccg gatccggcgc ccatctgcat ctctcgcttg gggagaagat tcccgcaggc
     4441 gcggttttcg atttcgaacc actcgggctg tggcggctcc gcccggagac ccggcatgcg
     4501 ccgattccct gcgatgtcgg gattctcctt ctggatcatg gtgtggagtg gggggtgaag
     4561 aagtggcgtc ccgaagatct cgtaaaccgg ctggactact ggtatgacgg agaaggacag
     4621 cgggtggttc tggtttgcga gaccaatccg gcggtacggt atgccagcat cgagttggcg
     4681 ttgacgcgga ccatcgtctt gcaggcgaac tgccgggatg tcacgtatga gggcctcgcg
     4741 gtgcgatacg gcggcgcaca tggtttcggc gggagccgga cacaacgcat caccatccgg
     4801 cagtgcgatc tgtcttggat cggcggtgga ttgcaggatt ggcgcaagag gaaaggaggt
     4861 gagcgtttcc cggtgcggta cggtaacgcc atcgagtttt ggaatggcgc ggaagatcat
     4921 gtcgtggaga gcaaccgtat ctgggaaatc tacgatgcgg cgttgaccaa tcagggccat
     4981 ggcgacgatt cgcgccagat gcgcatccga taccgccaca acgttatttg gaacgccgag
     5041 tattcattcg agttctggaa ccggccggaa acagcacggt cagaacacat tctgtttgag
     5101 tacaatacct gcgtcgatgc cggatccggc tgggcccaca agcagcgtcc tgatcccaac
     5161 ggcgcgcacc tgatgtttta cctcaactct tcggccacga ccaacgttgt gatccgtaac
     5221 aacctttttg tcggcaccac ggaagtctgt atgcgaatgg agaacgattg gcgcggaggg
     5281 ttgagcatgc ggaacaacct gtactggccg ggagacaaac cgttcctgcg ctggttgggc
     5341 aaaacgttct ttgaggccgg ggggtttacg cgctatcagc gggagttggc gttggactcg
     5401 aactcgctgc tggcggaacc gaagttcatg aatcccgcgg cccgtgacta ccggctgtgt
     5461 ccagacagcc ccggtgcgcg gctcgccacg gatggcggac cggtcggggc gcgctggtga
     5521 gatccgcgcg tcagggtgtt ggatccggcg cgggtgccgc gccgcgctcg cgcatccggt
     5581 taaccgctgg cgggaatcct tggtcggccg cccgcttgat ccagactgcg gcctcggacg
     5641 tgtccgggtc tgttccctga cccgcttcca gcatcaggcc gagccggaat tgcgcactcg
     5701 cgtgtccctg gtctgcggct ttccggaacc acgccagcgc cgcaccgtag tcctgcggca
     5761 cgccgatgcc cttgtagtag gccgcgccga gattgttctg gccatcggca tgcccgcctt
     5821 cggcggcttt ccggaaccaa ctcagcgcca actccgcatc ctgtttcatg ccctcgccgc
     5881 gactggccat caccccgagg ttggacatcg ccaatgcgtc atcccgaaac gcggcggcct
     5941 caaacaaacg gcgggcttcg tcgaacgatt gcagcacgcc tttgccgcgg taatacgcga
     6001 tgccgagttg cgtttgcgca cccgtatttc cctgttcggc ggctttgcgg taccagcgta
     6061 cgctttcggt gtcactctgc gcgaccacat aaccgttacg gtacatttgc cccaatgcca
     6121 cctgtgcggg tgcgtcaccc tgcccggccg cgcgtttcag ccagaccaca ccggcttcgg
     6181 aatcatgcac cgaacttgtt tgcgacaaga gcagggcacc cagactggat tgtgacacca
     6241 catgaccctg cgccgccgcc ttttccagcc agacggcagc ctcgcggtcg tcccggtcga
     6301 tatcaggcac gccttttaga aacagcaggc cgagtgccga ctgggctttg agatatccgt
     6361 tttcgcccga gcgttttaac cagaccagac cgctttccgc atcgcgcggc atgtcttcac
     6421 cgtaaagcag cgagaagccc agcttgtatt gcgaatcggg gtcgccggac tccgcttttt
     6481 cgcggagggt ttgcgtggga tcggacggcg cagagcgcca cgacaaagga aacgcgagcc
     6541 atttgaacga cgacgtccgg ttgattgaac accccccggc gaatatcgcg gccgccagtg
     6601 ccaaagcggc acccgcccac ttctccatcc tcatgcatcc tcctgaccga ctgcacgcaa
     6661 gcattccgcc aagcattggg gacaccggac gcgtccccgt ctcgcggcag atcctgcgcg
     6721 gtacggtcca ttaaaccttt ttcgccccga gcggtcaatc cggttgcaag aataggcagg
     6781 tattggatac acaacattat gaaaaaaaac ggacgtggat ccgcagatga aatcggttgt
     6841 aaaaccacat ctatggtagt at
//
LOCUS       DAOSRB010000152         6766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_10421_length_6766_cov_6.682909, whole genome shotgun sequence.
ACCESSION   DAOSRB010000152 DAOSRB010000000
VERSION     DAOSRB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10421_length_6766_cov_6.682909"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1596)
                     /locus_tag="PLZ74_08305"
     CDS             complement(178..1596)
                     /locus_tag="PLZ74_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOR98364.1"
                     /translation="MTKKTILTVVTSVTILTASAFDALKDRPTPPRQIFPEISAAQRD
                     ARRAWWTQSRFGMFIHFGLYASPARHEWVQSRERIAPDVYAKRYLPQFNPDLFDARDW
                     ARRAKQAGMKYMVLTAKHHEGFCMWDTKTTDYKITNTAFKRDLVREYVEAARAEGLRV
                     GFYFSIKDWHHPDYLIDATHPLRPADGRFTDEIYEKLNAGRDWNRYRTYMYDQVKELL
                     SDYGKIDIIWYDYTVKTKYGKNWKHWESVELLKLTRTLQPDILVDSRLDLMDTEDGWD
                     FVTPEQCRVTQWPQVDGKKAPWETCQTFSGSWGYYRDEETWKNPHQLIELLINSVSFG
                     GNLILNVGPTARGEFDYRACERLDAIGAWMRVNSRAIYDCTAAPDAFKKPVDSLLTYN
                     PETNRLYIHLVNYPMGYLPFDFADKVEYAQFLHDGSELKIRRSSTQVHSQSGDLGAHD
                     SFVLPMKKPNVEIPVIEIFLKK"
     gene            complement(1650..2675)
                     /locus_tag="PLZ74_08310"
     CDS             complement(1650..2675)
                     /locus_tag="PLZ74_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98365.1"
                     /translation="MTKDFPEVTSAEIKALAKRVGADCVGIAPMSRWEGVPKQNDPRY
                     IFPDAKSMIVLGFRIPRGLLRGIEEGTRFIDYPGMGYASINQVHGPMVLWKLNAYLEE
                     RGYEVFPLQNANGGDAVNPVTGKFREGLSRHVAEGLPLPDVLVHFRVAAYLAGLGEFG
                     WSKMFLTPEFGPRVRFAMLFTDAEFDTYDPIYAGKICDRCKLCVKHCGGNAISMNESV
                     KVTLAGRVVEWGKLDELACEEGLHGGEGRELSPFDGEYPRQYGYGRAVEGACGCIRAC
                     MVHLEQRNKLTKTFRNPFRKPDWKQWKVDHTKPYEIPPEVVAEYGLDKPQAKEESDAE
                     AFNPLND"
     gene            complement(2677..3750)
                     /locus_tag="PLZ74_08315"
     CDS             complement(2677..3750)
                     /locus_tag="PLZ74_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98366.1"
                     /translation="MAYEGELTAADVKAKAKECGADIVGIAPMSRWEGTDKQHDPRYI
                     FPDAKSMIVFGFRIPRGSLRGIEEGTHFLNYATMGYAALNSIYGPMVLWEMNRYLEDH
                     GYEAVPMANVSGGEAVNPLTGNFREGWSRPVKPGLPAPDVSVDFRVAAFLAGLGEFGY
                     SRAFLTPEFGPRQRFAIMLTDADLEPDPIYTGRICDRCMACVKHCYGRCISPTETVRK
                     VIGGVQVEWGKRDELACSRAAREGGLNRELSPFDGDYPRKYGYGLDHEGASGCIRACM
                     VHLEERAKMTRGFRRPFRKPGWKQWEIDHSKPYELPPEVRAEYAGNIESHDAYTNYNI
                     TSNYGTKAAAGLVQDDSSSGGVA"
     gene            complement(3756..5534)
                     /locus_tag="PLZ74_08320"
     CDS             complement(3756..5534)
                     /locus_tag="PLZ74_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008866738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOR98367.1"
                     /translation="MGYTVRFNFLDWMAIVLFFAALVGIAFGCSRKASKDAKDFFLSG
                     RSMPWWLIGVSMCAATTSTNSANMFTEFIRNSGLSENWKWWAFLLTGMLTVFVYSKLW
                     VRSGAKSDIEFYELRYSGRAAAFLRGFRALYLGIVFNVITTGLVMLAAIKIGQVLFGL
                     DQWTIIIVTSVCSIIYSALGGFRGAIYTDFLLFILIMIGAVVGMVYALGRPEVGGFAA
                     MMQNPVVQKHLSFLPDVNNTELFVSVFVIPIAIQWWNVWYPGSEPGGGGYIVQRMLSA
                     KSENHCVAGSILYQVINYAIRPWPWYLTAFASLIVFPDLASLQKAFPNVNPALVKGDL
                     AYPAMLTFVPNGWLGVVAASMMGALFSTVAAHLSMGSNYIANDFWKRFVRPDAGEREL
                     IIVGRVTVIVLMVLSGLLAPVLSSAGAVFNLILQIGAGTGLIYLLRWFWMRINAWSEI
                     TAMAVSFIVAVFLQLVYPHLGLPPLLSWHRLLIVMAITTVAWVGVTLVTPPTDAVKRA
                     SFQNKIRANKHDIAWGLLAMTIASVCVYALMFASGCWIYGKTVLAATMTVISIVFGLV
                     LIPVVRKLNAEQSDENCEFQTLNPGV"
     gene            5608..>6766
                     /locus_tag="PLZ74_08325"
     CDS             5608..>6766
                     /locus_tag="PLZ74_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOR98368.1"
                     /translation="MKAKVLLILPSDKFLHRQIIEGVLAHGREHGPWQFHFETGDRYE
                     QALGKGRNQACSGIIALVRERSQLAELLTLNKPIVLINPPSPLGGRQKAKPPPHAVYV
                     NRNQEDVGKTAAEYFLERGYRQFAFVGTPQAALWCERRLNGFCKRLADAGFSCNVYTS
                     KINTAADDFSLEAKALSSWLKKLHPGTALYVVRDRRALQVLEICSDIGISVPETISVL
                     STDNDVVLCESTSPSLSSIFLNGENCGQLCARLLDRLLRKRKVEPFVDLAFPQVITRQ
                     STDNTLIPDPFLAKALAIIRKDLSVPHTIEEIAQTLNVSRRTLELKARLVLGKTLKEE
                     IDSIRLNEAVRLLANSSEAIVKIASKCGFCGASHLNTRLKTVFGYGSSVFRY"
BASE COUNT         1484 a         2090 c         1858 g         1334 t
ORIGIN      
        1 gagatctgtc cggacagacg atctcagcct gcgcgactcg cgattcaacg accgtcagcg
       61 gcgtaagatt gcccgcccac gcaaaacacc ccaggaacag aattccgatt gctgcaggag
      121 atttcgattg tgtgaaaacc gtctgggtca ttgcctcgtc cgccctctgc catcccatca
      181 ctttttcaga aagatttcaa tgacgggtat ctcgacattc ggctttttca tcggcaggac
      241 aaaactgtca tgcgcaccga gatcgccact ctgtgaatgg acttgcgtcg aactgcggcg
      301 aatcttcagc tccgagccgt catgcagaaa ctgcgcgtat tcgactttgt ccgcgaagtc
      361 gaacggcagg tagcccatcg gatagttgac gaggtggatg tagagccgat tcgtttcggg
      421 attataggtg agcagcgaat caaccggctt cttgaacgca tccggcgcag ccgtgcaatc
      481 atagatcgcg cggctgttca cacgcatcca cgcgccgatt gcatcaagac gctcgcatgc
      541 gcgataatca aactcgccgc gggcggtcgg accaacgttg aggatgagat tgccgccgaa
      601 cgaaaccgag ttgatcagaa gttcgatcag ttggtgcgga ttcttccagg tctcttcatc
      661 gcggtagtag ccccacgaac cggagaacgt ctgacaagtc tcccacggtg ccttctttcc
      721 atccacctgc ggccactgcg tgacacggca ctgctcaggc gtgacgaagt cccatccgtc
      781 ctcggtgtcc ataagatcaa ggcggctgtc gacaaggatg tcgggctgga gggtgcgcgt
      841 cagcttcaga agttctaccg actcccagtg cttccagttc ttgccgtatt ttgtctttac
      901 cgtgtagtca taccagatga tatcgatctt gccatagtca gaaaggagtt ccttcacctg
      961 atcgtacatg taggtgcggt agcggttcca gtcccgcccg gcgttcagtt tctcgtagat
     1021 ttcgtccgtg aacctgccgt ccgcaggccg gagcggatgt gtcgcatcga taagatagtc
     1081 agggtggtgc caatccttga tcgagaagta gaaaccgacg cgaagccctt ccgcacgcgc
     1141 cgcctcgaca tactcgcgca cgagatcacg cttgaacgca gtgttggtga tcttgtaatc
     1201 ggtcgtcttc gtgtcccaca tgcagaatcc ttcatgatgc ttcgccgtca ggaccatgta
     1261 tttcatgcca gcttgcttcg cgcgtcgcgc ccaatcccgc gcatcgaaga gatcgggatt
     1321 gaattgcggc agatatcgct tcgcataaac gtcgggcgcg atccgttccc tcgactgaac
     1381 ccactcatgc cgcgccggcg acgcatagag accgaagtga atgaacatgc cgaagcggct
     1441 ttgcgtccac caagcccgtc gggcatctcg ttgcgcggcg gaaatctcgg ggaagatctg
     1501 ccgcggaggc gtaggccgat cctttagcgc gtcaaaggct gaagccgtca gaatcgtcac
     1561 agatgtcaca accgttagaa tcgttttctt tgtcatgttt tttcctttct agttcgatgc
     1621 aacttcttct cgttatgccc gtgttatcct cagtcgttca gcgggttaaa ggcttcggcg
     1681 tcggactcct ctttggcttg gggtttgtcg agaccgtatt ccgccacgac ttcgggcggg
     1741 atctcatagg gctttgtgtg atcgaccttc cactgtttcc aatccggctt acggaacgga
     1801 ttgcggaagg tcttcgtcag tttgttgcgc tgctcgagat gcaccatgca cgcgcggatg
     1861 cagccgcaag cgccttcgac agcacggccg taaccgtact gccgcgggta ctcgccgtca
     1921 aacggcgaca gttcgcgtcc ctcaccgccg tggagccctt cctcgcaagc gagttcgtca
     1981 agcttgcccc actcgaccac gcgtccagcc aaggtcacct tcacggactc gttcatcgag
     2041 atcgcgttcc cgccacagtg cttcacacag agcttgcagc ggtcgcagat tttgcccgcg
     2101 tagatcgggt cgtatgtgtc gaactcagca tccgtgaaaa gcatcgcgaa acggacgcgc
     2161 gggccgaact cgggcgtcag gaacatcttc gaccagccga actcgccgag acccgcgaga
     2221 tacgccgcga cgcggaagtg gacgaggacg tccggcaggg gaagcccttc cgcgacatga
     2281 cggctcagac cctcacggaa cttgcccgtg acaggattca ccgcatcacc gccgttcgcg
     2341 ttctgaagcg gaaagacctc atagccgcgc tcttcgaggt aggcattcaa cttccagagc
     2401 accatcgggc cgtgtacctg gttaatcgaa gcgtaaccca tgccgggata gtcgatgaag
     2461 cgtgtccctt cctcgatgcc gcggagaagc ccgcgcggaa tacggaagcc cagaacgatc
     2521 atcgacttcg cgtccgggaa gatgtaacgc gggtcgttct gcttcggcac accctcccag
     2581 cggctcatcg gcgcgatgcc gacacagtcc gcacccacgc gcttggcgag cgccttgatc
     2641 tctgcggacg tgacctctgg aaaatccttt gtcatatcag gcgactcctc cgctggacga
     2701 atcatcctgc acgagtccgg ccgcagcctt cgtaccgtag ttggatgtga tgttgtagtt
     2761 cgtataggcg tcgtgcgact cgatgttacc cgcatattcc gccctcactt ctggcggcag
     2821 ttcgtagggc ttcgaatgat cgatctccca ctgcttccag cccggcttac ggaacggccg
     2881 gcggaaaccg cgcgtcatct tcgcccgctc ttcgagatgc accatgcacg cccggatgca
     2941 accgctggca ccctcgtgat caagcccgta gccgtacttg cgcggatagt cgccgtcgaa
     3001 cggcgaaagc tcgcggttga gtccgccctc gcgagccgcc cgcgaacatg cgagttcgtc
     3061 ccgcttcccc cactcgacct gtacgccgcc gatgaccttc cgcaccgtct cggtcggcga
     3121 gatgcaacgc ccgtagcagt gcttgacgca cgccatgcag cggtcgcaga tacggcccgt
     3181 atagatcgga tcgggttcga gatccgcgtc ggtgagcatg atcgcgaagc gttgccgagg
     3241 gccgaactcg ggcgtcagga acgcgcgcga atagccgaac tcgccgagac ccgcaaggaa
     3301 cgcggcaacc cggaagtcga cggaaacgtc cggcgcggga agcccgggct tcacgggacg
     3361 gctccagccc tcgcggaagt tgcccgtgag cgggttgacc gcttcgccgc cggagacgtt
     3421 cgccatcggc acagcctcgt agccatgatc ttccagatag cggttcatct cccacagcac
     3481 catcggcccg tagatcgagt tgagcgcggc atatcccatc gtcgcgtagt tgaggaagtg
     3541 cgtcccctcc tcgatgccgc gcaacgatcc gcgcgggatg cggaaaccga agacgatcat
     3601 cgacttcgcg tccggaaaga tgtaacgcgg atcgtgctgc ttgtccgttc cctcccaccg
     3661 ggacatcggc gcgatgccga cgatatcagc cccgcactcc ttcgccttgg ccttcacatc
     3721 ggctgcagtc agttctcctt cgtatgccat aaccgttaaa ctcccggatt caaggtttga
     3781 aactcacaat tttcgtctga ttgttccgca ttcagcttcc tgacgacggg gatgaggaca
     3841 agaccgaaaa cgatggaaat gaccgtcatc gtcgcggcga gtaccgtctt gccgtaaatc
     3901 cagcagcccg aggcaaacat cagcgcatag acgcagacgc tcgcgatcgt cattgcaagg
     3961 agcccccacg cgatgtcgtg tttgttcgca cggatcttgt tctggaacga cgcgcgcttc
     4021 accgcatccg tcggcggcgt cacgagcgtc acgcccaccc acgcgaccgt cgttatcgcc
     4081 atcacgatca ggagccggtg ccaagaaagg agcggcggca ggcccagatg cgggtaaacg
     4141 agctgcagga acaccgcgac aatgaacgaa accgccattg cggtgatctc gctccaagcg
     4201 ttgatgcgca tccagaacca gcgcaggaga tagatgagcc ccgtgcccgc gccgatctgg
     4261 aggatcaagt tgaagaccgc gccggcggac gaaagaaccg gcgcaagaag ccccgagagc
     4321 accatcagca cgatgaccgt gacgcgtccg acgataataa gctcgcgctc acccgcgtcg
     4381 ggacgaacga agcgcttcca gaaatcattc gcgatatagt tcgaccccat cgagagatgc
     4441 gccgcgaccg tcgagaacag cgcgcccatc atcgatgcgg cgacgacgcc gagccagccg
     4501 ttcgggacga aagtgagcat cgccggatag gcgaggtcac ccttcaccag cgcggggttc
     4561 acgttcggga acgccttctg cagggaggcg agatccggaa agacgatcag cgaggcaaac
     4621 gccgtcaggt accagggcca cgggcgaatc gcgtagttga tgacctgata gaggatcgag
     4681 ccagcaacac aatggttctc gctcttcgcc gagagcatgc gctggacgat gtagccgccg
     4741 ccgcccggct cgctgcccgg ataccagacg ttccaccact ggatcgcgat cgggatcacg
     4801 aacacagaaa caaagagctc cgtgttattc acgtccggca gaaacgagag atgcttctga
     4861 acaaccgggt tctgcatcat cgccgcgaac ccgccgacct cgggacgacc gagggcatac
     4921 accatgccca ccaccgcacc gatcatgatc aggataaaga gaaggaagtc cgtatagatc
     4981 gcaccgcgga accccccaag cgccgaatag atgatcgagc agaccgacgt cacgatgatg
     5041 atcgtccact ggtcgagccc gaagagcacc tggccgatct tgatcgccgc gagcatcacg
     5101 agccccgtgg tgatcacgtt gaacacgatc ccgaggtaca gcgcgcggaa accccgcagg
     5161 aaggccgcag cccgccccga gtagcggagc tcgtagaact cgatatcgct cttggcgccg
     5221 gaccgcaccc agagcttcga gtagacgaac accgtcagca tcccggtcag gaggaacgcc
     5281 caccacttcc agttctcgga aagacccgag ttgcggataa actccgtgaa catgttggcg
     5341 gaattcgtag acgtggttgc ggcgcacatg gatacgccga tcaaccacca tggcatcgac
     5401 cgcccggaaa ggaagaaatc cttcgcgtcc ttcgatgctt tgcgcgaaca gccaaacgcg
     5461 ataccaacaa gcgccgcgaa aaacaggacg atcgccatcc agtcaagaaa attgaatctg
     5521 acggtgtatc ccatttgata gaattgtagc gaaaacgcat tgcccttaat agacataaac
     5581 gcgaaaaata tatacaataa atgcgaaatg aaagcgaagg ttctgctcat cctgccgtcg
     5641 gataagttcc ttcaccggca aattatcgag ggcgttctcg cgcacgggcg cgagcatggc
     5701 ccttggcagt ttcacttcga gaccggggac cgttacgagc aggcgctcgg caagggtcgc
     5761 aatcaggctt gctccggcat catcgccttg gtgcgtgaac gaagccaact cgcagaactg
     5821 cttacgctga acaaacctat cgttctcatc aatccgccct ccccgctcgg aggacgtcaa
     5881 aaggcaaaac caccaccgca tgccgtttat gtcaaccgga atcaggagga tgtcggcaaa
     5941 accgctgcgg agtattttct ggaacgcgga taccgacagt tcgcctttgt cggaacacct
     6001 caagccgcac tctggtgcga gcgccgttta aacggatttt gcaagcgtct ggctgatgcc
     6061 ggcttttcct gcaacgtcta cacctccaag atcaatactg cggcagatga cttctctctg
     6121 gaagccaagg cgctctcatc gtggttaaag aaactgcatc cgggaaccgc cctttacgtc
     6181 gtacgtgacc ggcgagcact tcaggttctg gagatctgct ccgatattgg catctccgtg
     6241 cctgaaacga tatcggttct cagcacagac aatgatgtgg ttctctgcga atcgacaagc
     6301 ccctcgctct cttccatctt tctgaacggc gaaaactgtg gacagctctg cgcacgactt
     6361 ctggatcggc ttctccgcaa acgcaaggtc gaaccctttg tcgaccttgc gttcccgcag
     6421 gtcataaccc gccaatcgac tgacaacacg ctaattccag accctttcct cgcaaaagca
     6481 ctggcaatca tccgaaaaga cctatccgtt ccgcacacca tcgaagaaat cgctcaaacg
     6541 ttgaatgtct ctaggcgtac tctggaattg aaggcccggc tagtcctcgg aaagacgctg
     6601 aaagaggaaa tcgacagcat ccgacttaac gaagccgtca gacttctcgc caactcatcc
     6661 gaagcgatcg tgaaaatcgc ctccaagtgc ggtttctgcg gcgcgagcca tctcaacaca
     6721 cgccttaaga cggtctttgg ctacggttcg tctgttttcc gctacc
//
LOCUS       DAOSRB010000153         6765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_10422_length_6765_cov_7.121610, whole genome shotgun sequence.
ACCESSION   DAOSRB010000153 DAOSRB010000000
VERSION     DAOSRB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10422_length_6765_cov_7.121610"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(433..627)
                     /locus_tag="PLZ74_08330"
     CDS             complement(433..627)
                     /locus_tag="PLZ74_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98369.1"
                     /translation="MKLDAEFCESRCPVCVNARKGNKLAIGLQKLEMFLTGGGCPAGR
                     DRLKKYGVRPNEKRSDRVVS"
     gene            complement(771..1136)
                     /locus_tag="PLZ74_08335"
     CDS             complement(771..1136)
                     /locus_tag="PLZ74_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HOR98370.1"
                     /translation="MNETANVGMMGMFPLIITLALCLLMLASMWRIFTKAGQPGWAIL
                     IPIYNAYVMLKVAGKPGWWLLLMLIPVVNLIVTILMLAGIAKNFGKGVGYVLGLIFFP
                     ILFYPMLAFGDAQYRGVAG"
     gene            complement(1841..3184)
                     /locus_tag="PLZ74_08340"
     CDS             complement(1841..3184)
                     /locus_tag="PLZ74_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOR98371.1"
                     /translation="MAIDGKALALACFKAGVAGVAPDRLVRDHVRSDERTLTICGQEF
                     DPSRIERIFVVGAGKASGLMALALEEMLGDRVIGGAVVVKHGHAVPCRKIRIMEAAHP
                     YPDQAGVDATQKIMRFCEEAREGDLMLCVWSGGGSALLADAPEECSVEEVARLSEVLV
                     TSGADIGEINAVRKHLSRVKGGQLARLAWPARVVSLILSDVVGDPLDVIASGPTVADP
                     TTFGEALAVLRKYRIEAQTPPQLLQVLNNGAAGLLPETPKADDPALARTVNSIIGNNR
                     IALEHVAQYAQSRGLSVRIVTDRLEGATAEAAATIVRAACEAQSDTSLAKPACLLFGG
                     ETTLRVMGCGTGGRNQHLALQAARLLRGRKGITLLAGGSDGTDGPTEMAGAVVDGETC
                     VAAERANVDAAAYLRAFDSYAFFKKIGGHVFTGPTRTNVMDLIVVILGADASPAP"
     gene            complement(3184..5451)
                     /locus_tag="PLZ74_08345"
     CDS             complement(3184..5451)
                     /locus_tag="PLZ74_08345"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98372.1"
                     /translation="MRERMRCLILVCLNGLCLWAAAGLLEQQELTGLDEIVFVKRHTY
                     NANHYYTEFINSTWMPGGNICVLNLKTGAVRELLPQMTGGVFGRFDLSFDARRVVFDW
                     KPGPQEGCRIFEVNIDGTGLRQLTFPQDDEAELSAAYRKSPKYHHGTDDMHPCYLPDG
                     GIAFVSTRCQYGILCDPADEFTTTVMFRMDGDGKNMRQLSNSSVSEACPVMLEDGRLM
                     YTRWEYVDKGAVAVKCLWAMRPDGTASQEIYGNDIAVPTTMLYGRPIPGMADAFVFLG
                     TPHYPHNGFGTVVRLDMSKPIRTELPMTYITADVDIKTEGGFHFRDSAGGWQRDPEGK
                     GRLFKDPYPLSAALYLVSHKPAGEVWRSPAGYGLYVLNEAGEVAEIYRDPAISCFLPY
                     PLRARSVPPVVHSPVNSALAAAGEAVCLVQDVYHGLTGVPRGAVRWLRVLEQVPRPWA
                     AQMADNSDEYDQQHIVISKDTHLGLKVQWGVVPVDPDGSAHFKVPAGRNLFFQALDAD
                     YLMLQTERTYVNYMPGEVRSCIGCHETPQSAADYQRKDRRRRARPSAFGRAPDVPGPQ
                     PNEERGQRTLDYMTDVQPLFDRHCISCHGESAGEPGKLDLRGKLTRKFCVSYEALVPE
                     RRKGSFYRGLLGTVIGENHPKTGNVDYLPPGTLGARTSVLAAMLGRGKIVLADTANQA
                     RAASLAQSHVKALARLTPEEFLRLANWIDTNCQYYGSYWGRRDLKHKDSPFFRPSATF
                     EQAISREFPWPGGMR"
     gene            complement(5612..6277)
                     /locus_tag="PLZ74_08350"
     CDS             complement(5612..6277)
                     /locus_tag="PLZ74_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOR98373.1"
                     /translation="MRISNDNDLAEACRKLRCADKRLAEIINHVGPCTLKPHTPDFHF
                     LADAIIGQQIATKAAATISARFKQLFKGNKPTAKGFLNIPKEEVLSAGVSGQKYGYIL
                     DLAQRINSRKLRLASLSKMSDSGVREKLTEVKGIGVWTADMFLLFGLGRLDVFPLHDF
                     ALRQAMARVYDVPADDHGALLRIANRWAPYRSVGTWYMYRSKNAQQGSQRSGTRSRAR
                     GRE"
BASE COUNT         1491 a         2196 c         1704 g         1374 t
ORIGIN      
        1 acttgttgac gttcagcggg ttaaaggtgt tgcaatagct aatggctaac gtggattgtt
       61 taaccacaga acactttctt gggtttcaag gtttcagggt ttctgggttt ctggtttcag
      121 gatttcatcc aaatcgctat cgggatcgct atcggggtcg ggatcgggat cgctatcggg
      181 gtcgggatcg ctatcgctat cgggatcgac catttaacaa ccgcctcaca gcctcacagc
      241 ctcacagcct cacagccttt gagattgcgg gctccgcccg catctcaata cgtcaaaacc
      301 caatgtttac gggggtttac tagaattcta cccgttcaac tgctcctttt tggttgaacc
      361 taaaaaagag cagcggaatg caaaccccgt cctcctcttc ccgaagaggg agccgttcag
      421 cggcagggat cgtcatgaca ccactcggtc cgaccgcttc tcgttcgggc gcacaccgta
      481 ctttttcagc cggtcgcgcc cggcgggaca cccgcccccg gtaagaaaca tctccaactt
      541 ctgcaaccca atcgccagtt tgttgccctt gcgggcgttg acgcagacag gacaacgcga
      601 ttcacagaat tcggcatcga gtttcatagg accttcttcc cttctgacac ggacgaagcc
      661 cgcaacccaa ggaatcagga gtccgaagcg aatgaatcga tcatgtcgcg actctcgatt
      721 ctgactcctg cgagaaaacc tgtcccacgc ctgcgggatg gctcatctta ttaaccggcc
      781 acaccgcgat actgcgcgtc accgaatgct aacatcggat agaaaaggat cggaaagaag
      841 atcagtccca agacatatcc tacgcctttc ccgaaatttt tggcgattcc agccaacatc
      901 aggatcgtga cgatcagatt aacgaccggg atcagcatca gcaacagcca ccagcccggc
      961 ttacccgcga ccttcagcat cacgtacgca ttatagatcg gaatcaggat cgcccatcca
     1021 ggctgtccgg ctttggtgaa gatacgccac atcgacgcca gcatcaacag acagagcgcc
     1081 aacgtgatta tcagcggaaa catgcccatc atccctacgt ttgccgtctc attcatattc
     1141 cacgtccttt ctgggtttcg gggctgtcaa agcacgaaat gcgtcctttg aaacaacccg
     1201 ctctttttag gataccccct gccgacccgt ccggcaagtg cattctcgcg tgtcccgcgt
     1261 catttcatcc ttcaccctaa aaagagcagt tgaacgggta gaaattctag caaccccccg
     1321 caaacattgg gttttgacgt attgagatgc gggcggagcc cgcaatctca aaggcggtga
     1381 gactgtgagg cggtgaggcg gttgttaaat ggtcgatccc gatagcgata gcgatcccga
     1441 ccccgatgaa atcctgaaac cgtgaaacca tgaaaccctg aaacccaaga aagtgttctg
     1501 tggttaaaca atcgattccg atagcgatcc cgaccccgac cccgatttgg atgaaaccca
     1561 gaaaccccga aaccatgaaa cccgaaaccc tgaaaccatg aaaccgtgaa acccaagaaa
     1621 gtgtcccgtg gtgaaacagc cgatcccgat agcgatcccg atcccgatac cgatttggat
     1681 gaaacccaga aaccccgaaa ccatgaaacc cgaaaccctg aaaccatgaa accgtgaaac
     1741 ccaagaaagt gttctgtggt gaaacagccg atcccgatag cgatcccgat agcgatcccg
     1801 ataccgatac cgatttggat gaagccaaga acctcaccgg tcagggggcc ggggatgcgt
     1861 cagcgcccag aatcacgaca attaagtcca tgacgttggt ccgcgtcggg ccggtgaaca
     1921 catgaccacc gatttttttg aagaacgcgt acgaatcaaa ggcgcgcaga tacgccgccg
     1981 catccacatt ggcccgttcg gccgcaacgc acgtttcccc gtccaccacc gcgcccgcca
     2041 tttccgtcgg cccatccgta ccgtccgaac cgccagccag cagagtgatc cccttgcgcc
     2101 cgcgcagcag ccgtgcggcc tgaagcgcga gatgctggtt acggccaccc gttccgcaac
     2161 ccatgacccg caacgtggtt tcgccaccga acagcagaca ggcgggcttg gcgaggctcg
     2221 tgtcggactg cgcctcgcac gcggcccgca cgatcgttgc ggccgcctcc gcagtcgccc
     2281 cttcgaggcg gtccgtcacg atcctcaccg acagcccccg actctgcgca tattgagcga
     2341 catgttccag cgcaatccgg ttgttgccga tgattgagtt caccgttcgt gccagcgcgg
     2401 gatcatccgc tttcggcgtt tcgggcagaa ggcctgccgc gccgttgttc aacacctgca
     2461 gaagctgtgg gggcgtttgc gcctcgatcc ggtatttccg gagaacagcc aacgcttccc
     2521 cgaacgttgt ggggtctgcc acggtcgggc ccgaagcgat gacatccaaa ggatcaccga
     2581 ccacatccga aagaatcagg ctcaccaccc gcgcgggcca ggcgagccgc gccaactgtc
     2641 cacccttcac ccggctgaga tgcttgcgga ccgcgttgat ctcgccgatg tcggccccgg
     2701 acgtcaccaa gacctccgac aggcgcgcca cctcctccac cgagcactcc tccggcgcgt
     2761 cggccagcag cgcggaaccg ccgcccgacc aaacgcacag catgaggtct ccctcgcggg
     2821 cctcctcgca aaaccgcatg atcttttgcg tggcgtcaac gcccgcctga tccggataag
     2881 gatgcgccgc ctccatgatc ctgatttttc ggcaaggcac cgcatggcca tgcttgacga
     2941 caaccgcacc gccaatcacc cggtcaccca gcatctcctc cagcgccaac gccatgagtc
     3001 cagacgcctt ccccgcgccg accacgaaaa tccgctcgat gcggcttgga tcgaactcct
     3061 gcccgcagat cgtcagcgtc cgctcatcag atctgacgtg atcgcgcacc agccgatccg
     3121 gggcgacgcc cgcgacaccg gccttgaagc acgccagcgc gagcgctttt ccgtcaatag
     3181 ccatcaccgc atcccccccg gccatggaaa ctcgcggctg atcgcctgct caaacgtcgc
     3241 tgacggacgg aaaaacggcg aatctttatg tttcaggtca cgccgccccc aataagagcc
     3301 gtaatactga cagttggtgt cgatccaatt ggccagccgc aaaaactcct cgggggtcaa
     3361 ccgtgccaat gctttgacat gcgactgcgc cagagaggcc gcccgcgcct ggtttgccgt
     3421 atccgccagc acaatcttac cccgtcccag catcgccgcc aaaacactgg tgcgcgcccc
     3481 cagcgtgccc ggcggtaagt aatcgacatt gccggtcttg ggatgattct cgccgataac
     3541 cgtgccaagt aaaccgcggt aaaaagagcc tttgcgcctc tccggaacca gtgcctcgta
     3601 tgagacgcag aatttgcgtg tcagcttgcc gcgcaaatcc agctttcccg gctcccccgc
     3661 gctttcgccg tgacaggata tgcagtgccg gtcaaacagc ggctgaacgt ccgtcatgta
     3721 atccagcgta cgttgaccgc gctcttcgtt gggctgcggg cccggcacat ccggcgcccg
     3781 cccgaacgcg ctcggccgcg ccctgcgccg cctgtcttta cgctggtaat ccgccgcgct
     3841 ttgcggcgtt tcatggcaac cgatacagga acgcacctcg cccggcatgt agttcacata
     3901 cgtccgttct gtctgcaaca tcagatagtc ggcatccagc gcctggaaaa acagattacg
     3961 cccagccggc accttgaaat gtgccgagcc gtccggatca accggcacaa cgccccactg
     4021 caccttgagc cccagatgcg tgtccttgct gatcacgatg tgctgctggt catactcgtc
     4081 actgttgtcc gccatctgcg ccgcccacgg ccggggcacc tgttccagca cgcgcagcca
     4141 cctgaccgcg ccgcgcggca ctcccgtaag cccgtggtat acatcctgca ccagacagac
     4201 agcctccccc gccgcagcca atgcgctgtt cacaggcgaa tgcacaaccg gcggcacact
     4261 tcgcgcccgc aaaggataag gcaggaaaca ggatatcgcg ggatcgcggt agatctccgc
     4321 gacttctccc gcctcgttca aaacataaag gccataccct gccggtgacc gccacacttc
     4381 gcccgctggc ttgtgcgaga ccagatagag cgcggcagac aacggatacg ggtctttaaa
     4441 gagccgcccc ttgccctccg gatctcgctg ccacccgcca gcgctgtccc ggaaatgaaa
     4501 cccgccctcg gttttgatat ccacgtcagc cgtgatataa gtcatgggca actccgtgcg
     4561 gatcggcttt gacatatcca gtctgaccac tgtgccgaac ccgttgtgcg ggtaatgcgg
     4621 agtaccgaga aacacgaagg catccgccat acccgggatc ggcctgccgt aaagcatggt
     4681 tgtcggcacc gcgatgtcgt tgccgtagat ctcctgcgag gccgtgccgt ccggacgcat
     4741 cgcccacaaa catttcaccg ccaccgcgcc cttgtcgaca tattcccacc gggtgtacat
     4801 cagccgcccg tcctccagca tcaccggaca cgcctcgctg acagaactgt tggaaagctg
     4861 ccgcatgttc ttgccgtcgc catccatgcg gaacatcacc gttgtggtga actcatcggc
     4921 cggatcgcac agaatgccgt attgacaacg ggtcgagaca aaagcgatcc cgccatccgg
     4981 caggtagcac gggtgcatat cgtctgtgcc gtgatgatac ttgggactct tgcggtaagc
     5041 cgctgaaagc tctgcctcgt cgtcctgagg aaacgtcaac tgccgcaatc ccgtgccgtc
     5101 aatgttgacc tcaaaaatgc ggcagccttc ctgcggcccg ggcttccagt cgaaaaccac
     5161 acgccgcgcg tcaaacgaaa gatcaaaccg gccgaacacg ccgcccgtca tctgcggcag
     5221 cagttcgcgc accgcgccgg ttttaagatt caacacacag atattaccgc ccggcatcca
     5281 tgtgctgttg atgaattccg tatagtagtg attggcgttg tacgtgtgcc gcttgacaaa
     5341 aacgatttca tccagccccg tcaactcctg ttgctccagc aaaccggctg ccgcccacag
     5401 acacaacccg tttaggcaaa ctaaaatcaa acagcgcatc cgttcacgca tacgatttct
     5461 cctatacatg caccccgctc ccaagatgcc tcgccagtcg ttccatgcgg cttgaggtac
     5521 gattatttta ccataaacgc tgttcccgga actacgatct tttagctcct ccttgtcctc
     5581 gaaacacggc ctcctgaatt tatatcgaac ctcattcgcg tcctcgtgca cgtgagcgtg
     5641 tgccgctacg ctgtgaacct tgttgggcat ttttacttcg atacatatac cacgttccca
     5701 cactgcgata aggagcccag cggttggcta tgcgcaacaa agccccatga tcatcggctg
     5761 gcacatcata gactctagcc attgcctgcc ttagggcgaa gtcgtgcaaa ggaaagacgt
     5821 ccagcctgcc gaggccaaaa agaagaaaca tgtcagcggt ccagaccccg atgcccttca
     5881 cctccgtcaa tttttcgcgt actccggaat cgctcatctt agagagcgag gccaaacgca
     5941 gcttccggct gtttatacgt tgggcaaggt caagaatgta gccatatttt tgcccggaca
     6001 cgccagccga aagcacttct tcttttggta tgttgagaaa gccctttgcc gtgggtttgt
     6061 ttcctttgaa aagctgcttg aatcgggctg aaatcgtggc ggcagctttc gtggcgattt
     6121 gctgaccgat aatcgcatca gccagaaaat gaaaatccgg tgtatgcggc ttcagcgtgc
     6181 acggcccgac gtggttgata atttcagcaa gccgcttgtc tgcgcaacgt aacttgcggc
     6241 atgcctcagc cagatcgttg tcgttagaaa tgcgcattgt cagctcatga atacccaatg
     6301 tagagcaagg ctctccaatg accgtatgtt gtggggcttc accgtttcat gcgggcgccg
     6361 gggctaatcg gatgttgctt cgcgtctgcg tgggcattga ttacaatctt gtttctacct
     6421 gcatcaatcg cagtctggat aatcgtctca atggattcgg taacgaaggt tgagcattcg
     6481 tcatagtcat cgcattgctt tggcaccttg aatgtcaaat cctcatactt cacgcgcaat
     6541 cccctctgtt ctcgctgcct tgcttcccgc ccaacaatgc agtgccactg aacgcactcc
     6601 gcgatggcga ccctatccgt gcggtccagt ttacggactc ctccccgaac ctgtcaagcc
     6661 gcgcccccat tgaaacatga ttgaggcctg cgcgcatgtg cacacgcgtt atcccgaccc
     6721 cgaccccgat ttggatgaaa ccttgaaacc ttgaaaccag aaacc
//
LOCUS       DAOSRB010000154         6594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_10747_length_6594_cov_7.187185, whole genome shotgun sequence.
ACCESSION   DAOSRB010000154 DAOSRB010000000
VERSION     DAOSRB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10747_length_6594_cov_7.187185"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5084)
                     /locus_tag="PLZ74_08355"
     CDS             complement(<1..5084)
                     /locus_tag="PLZ74_08355"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOR98374.1"
                     /translation="MKTTDMKTGIKKSVLPWVVCGVLWAGVTARAQYFPPSNGDLIGV
                     LTSVSITNKTLSNVGTAIPIELRLWGHFMLTNTAVGPIAMPRLRMNLAAEEEAWATLE
                     DPADVQRFTDGVGQKTRVTFKYRIREGDMVSKLKISGSDGSDVLGVPYGFDWCNDTWK
                     IVRVSDPASNAVWRFRTTSPRYLELPGDDPTHYDFSYRSVGIKTLQFSSAPSPVQVGE
                     SVTWAVSTVNPVSSGRTVSFYVWVSNTVSQGDVNLNGVKSQKLMIAGGSTTATFQITG
                     LTVGTNTVFLQGTQDYDNNASLGVQNAETRQIVVAPAPEPYVSVLLPNAPAPHNEQTL
                     PETSAFGTGALQVRLSQVYTEDVWVRLDTTLNGGPQSNNVTFSETPKFVRVRAGNHNS
                     DTVAFNVPDGIHASELFGITIMPVITNAAIDAVYSGESVPCIVKVANVAPVVQTPQNG
                     ATATAKRGTPQRFTWSVSDVAADLTGMTVTWDFGDGSALVTTNGYFGEIFHTYTSNGS
                     REVKVFATDKDGKDSAVITMTVTVEDPVPAPKLSLLFSTGTTNTASYTETATPLTGWV
                     RLVLSESFYESVQISLKALPDGQGNLLLGTTNLITIANGDLESGNIPFSLLDGTPDSS
                     VNGITLDPVIVTPNASNYYTDVRQGTVRVQNVVPVIMEPVGYATVAEATTNAAVAPYD
                     AIPMGRSFGFSWALDDVVADSSGLVLDWDFGDNTSQSVTNGLIGKVFHTYSSLGEKIV
                     RLQVTDKDGGKTTRIAFKVNVVPPPPEPTVYVRPPAGYLTETTVMGSGSIDVELSEAF
                     TEKVTVRLAVSPSASDTNGEILLATNLVEFRVGEVFKTVKVSARDGTVASEINGFTVT
                     PSVIGTAAAINHYTELWPGEVQVVNADPVIRTPLDAPDSTTISYTIPQGTGWKFFWDV
                     QDVPADLPGMKVTWYFGDGSSETLTGGSGSVTHTYNAPGIMSVRVVAKDKDGGSDEVN
                     FKVEVRPAKSVIVTPIGPNLEANYWGAPGLGNGMVFSEGALGRINRNNTYFFTYGPGA
                     SSAALRAVPYKTGPSGWYTVTNFNNEGAAIPGAQHHFDSFFYVWVGEDQGLPAQDIKP
                     SQASPTTALTLPASAGGTESVDIRIIQAIFSREYRAADNCGDINQDGIPDKIAAQYNL
                     PVIARGGTDGGGGAGSTGEMPIELTPVGDFNRDKDAAGNEVGDFLPGAASGGGAIIGG
                     ISNVFAAVGDPFTAFLEVRGFHPGLNSALFNSDDDFGENETSRDCERPTNPTLQDTDG
                     DLFPDGWEYYFWYNAKVNGVTGMAYTPTNIAVGTLIPTRTITTAFDPIVPATDSATGA
                     AVNRDLDNDGLSDIEELTIGTNPIHWDTDGDGMCDGWEVMRGLNPNDSRDGLNAMMDN
                     PDGDYMAYALVPRLLVTLENGVQVLAPAAAVVSAGEALGEVEGLTIPYRYGDDTAPIA
                     VGRPATGLAEEVVGSVEPIDAVIVHNQVMQEFGFDPRTAWAGTVNPLSNPQRFPEWVA
                     GLTAENVITAPNTRRFTSKDEYLLMKYMAELRLNGCTGAIGDGNAAVKTADWSIYSTH
                     PKTPDSDVVWGDGGAVVKTDRMPDGWELYVSLPPGSEGTETDMLISPWDPYDGDWTFV
                     GEDNLHNNREFHGTDCSAGYTNVALYGVYPGDTPVVTIVRPESDALWINKFWPSNPYA
                     GDTDGDGLKDDAEMTFIYGTPVDNGTT"
     gene            complement(5133..5648)
                     /locus_tag="PLZ74_08360"
     CDS             complement(5133..5648)
                     /locus_tag="PLZ74_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98375.1"
                     /translation="MRIAFECNGWKIRECGVAVRLGTAFLSIVFSLFFLNGCSKRRPP
                     AEEALPEVITNRMADAAYRAELAELRREQTRLAGGRAEVVEQMRVMVERARATLPEGA
                     DDAAVKAVLVKDPEWNRLENANQEWIGKIQDNLAQARERVRQRMLAEERDNRRVAEGT
                     ARPAAGETAAP"
     gene            5820..>6594
                     /locus_tag="PLZ74_08365"
     CDS             5820..>6594
                     /locus_tag="PLZ74_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOR98376.1"
                     /translation="MTQDRTNNVAAETLVLADGENQQSLLRVRRIKLLVISGPLQGRE
                     ILVNRDTFTIGSGSHNDLIVEDSTVSKRHCEIVVEQSGYLIRDLESTNGTLVQGVRVS
                     SAHLPPGSEIQLGKTRIVFCPLQEAHDIPLSNREAFGAMLGSSVPMRRIFYLAETYSP
                     ADVTVMITGETGTGKEILAEEIHNHSPRHDKPFIVIDCAAISKELIESELFGHVKGAF
                     TGANADRQGAFELADGGTVFLDEIGDLSPDLQPKLLRVLE"
BASE COUNT         1181 a         2149 c         2016 g         1248 t
ORIGIN      
        1 gtggtgccgt tgtccacagg cgtgccgtag ataaaggtca tctcggcatc atccttcagg
       61 ccgtcgccgt ccgtatcgcc cgcatagggg ttggagggcc agaatttgtt gatccagaga
      121 gcgtccgatt cgggccgcac gatggtcacg accggggtgt cccccggata cactccatac
      181 agcgcgacgt tggtgtaccc ggcggagcag tccgtaccgt ggaactcgcg gttgttatgg
      241 aggttgtcct cgccgacaaa ggtccagtcg ccgtcgtacg gatcccacgg cgagatcagc
      301 atatcggttt ccgtgccctc agaccccggc ggcagggaga cgtacagttc ccatccgtcc
      361 ggcatgcggt cggtcttgac caccgcgccg ccgtcacccc agaccacgtc gctgtccggt
      421 gtcttcgggt gggtggagta gatcgaccag tccgcggtct tcacggccgc gttgccatcg
      481 ccgatggcgc cggtgcaacc gttgagacgc agctcggcca tgtatttcat cagcagatat
      541 tcgtctttgg aggtgaagcg ccgcgtgttc ggcgcggtga tcacattttc ggcggtcagc
      601 ccggcaaccc actccggaaa acgctgcggg ttggaaagcg ggttgacggt gccggcccac
      661 gccgtgcgcg ggtcgaagcc gaactcctgc atcacctggt tgtggacgat caccgcatcg
      721 atcggctcca cgctccccac aacctcctcc gccaacccgg tggcgggacg gccgaccgcg
      781 atcggcgcgg tgtcatcgcc ataccggtag gggatcgtca ggccttcgac ctcgccgagc
      841 gcctcgcccg cagagaccac ggccgccgca ggcgccagaa cctgaactcc gttttcgagg
      901 gtcacgagca gccggggcac cagggcgtag gccatgtagt cgccgtccgg gttatccatc
      961 atcgcgttca gaccgtcacg cgaatcgttc gggttcaagc cgcgcatcac ctcccagccg
     1021 tcgcacatgc cgtcgccgtc cgtatcccag tggatcgggt tcgtgccgat cgtcagctcc
     1081 tcgatgtcgc tcagcccgtc gttgtcgaga tcgcggttga ccgccgcgcc ggtcgcggag
     1141 tcggtcgcgg gcacgatcgg gtcaaaggcg gtggtgatgg tgcgggtcgg gatcagcgtg
     1201 ccgaccgcaa tgttggtggg cgtgtaggcc atgccggtga caccgttgac cttggcattg
     1261 taccagaagt aatactccca tccgtccggg aagaggtcgc cgtccgtatc ctgcagggtc
     1321 gggttggtgg gccgctcaca gtcgcggctg gtctcattct cgccgaaatc gtcgtcggaa
     1381 ttgaacaacg cgctgttgag tccggggtgg aagccgcgca cctccaggaa ggctgtgaac
     1441 ggatcgccga ccgcggcgaa gacattggag atcccgccga tgatggcgcc gccgccgctc
     1501 gccgcgccgg gcaggaagtc gccgacctca ttgccggccg catccttgtc gcggttgaag
     1561 tccccgacgg gcgtgagttc gatcggcatt tcgccggtcg agccggctcc gccgccgccg
     1621 tcggtgccgc cacgcgcgat cacaggcagg ttgtactggg ccgcgatctt atcgggaatg
     1681 ccgtcctggt tgatgtcgcc gcagttgtcg gccgcacggt actcgcggct gaagatcgcc
     1741 tgaatgatgc ggatgtccac cgactcggtg ccgcctgcgg aggccggcag ggtcagcgcc
     1801 gtggtgggac tggcctgtga gggtttgata tcctgagccg gcaggccctg atcctcgccg
     1861 acccacacat agaagaaact gtcaaagtga tgctgggctc ccggaatcgc ggcaccctca
     1921 ttgttgaagt tcgtcacggt ataccatccg gaggggccgg tcttgtacgg cacggcgcgc
     1981 agcgccgccg acgaggcacc ggggccgtag gtgaagaagt aggtgttgtt gcggttgatg
     2041 cggccgaggg ccccctccga aaagaccatc ccgttgccca gacccggcgc tccccagtag
     2101 ttggcctcca aattggggcc gatcggcgtg acaatcacac tcttggcggg cctgacttcc
     2161 accttgaagt tcacttcgtc gcttccgcca tccttatctt tggccaccac acgcacggac
     2221 atgatgccgg gggcgttata cgtgtgcgta acggaacccg aaccgccggt gagcgtctcg
     2281 ctggaaccgt ccccgaaata ccaggtcact ttcatgccgg gcaggtccgc gggcacatcc
     2341 tgcacatccc agaaaaactt ccagcccgtt ccctgcggaa tggtgtacga gatcgtggtg
     2401 gagtccggag cgtccaacgg ggtccggata accggatcgg cattgacgac ctgcacttct
     2461 cccggccaca gttcggtata atggttgatg gccgccgccg taccgatcac cgacggcgtg
     2521 acggtgaacc cgtttatttc cgacgccacg gtgccgtcgc gcgcgctgac tttgacggtt
     2581 ttgaagactt cacccacgcg gaattcgacc agattggtcg ccagcaggat ctccccgttg
     2641 gtgtcactgg cagacgggct gaccgctaac cgcaccgtca ccttttccgt gaaggcctcg
     2701 gagagttcga catcgatcga gcccgaaccc atgacggttg tttccgtgag gtacccggcg
     2761 ggcgggcgga catagaccgt gggttccggc gggggcggga ccacattcac cttaaaggcg
     2821 atgcgcgtgg tcttgccgcc atccttgtcc gtaacctgca gccggacgat cttctcgccg
     2881 agcgagctat aggtgtggaa caccttgccg atcaggccgt tggtcacgct ctgcgaggtg
     2941 ttgtcgccga aatcccagtc cagaaccaga ccgctggagt cggccaccac atcatcaagc
     3001 gcccaggaga atccgaacga ccggcccatg gggatcgcgt cgtagggggc cacggcggca
     3061 ttggtggtgg cctcggccac ggtggcgtat ccgaccggct ccatgatcac gggaaccacg
     3121 ttctgcacgc gcaccgttcc ctgacgcacg tccgtatagt aattggaggc gttgggcgtg
     3181 acgatgaccg gatcaagcgt gatcccgttc acgctgctgt cgggggtgcc gtccagcagg
     3241 ctgaagggaa tgttgccgga ctccagatcg ccgttggcga tggtgatgag gttggtggtg
     3301 cccagcaaca ggttgccctg gccgtccggc agggccttca ggctgatctg tacactctcg
     3361 tagaaggact ctgaaagaac cagccgcacc cagccggtga gcggggtcgc cgtctccgtg
     3421 taagaggcgg tgttggtggt gcccgtcgag aacagcaggg agagtttcgg agccgggacg
     3481 ggatcctcga ccgtgacggt catcgtgatg accgcggaat ccttaccgtc cttatccgtg
     3541 gcgaaaacct tcacttcgcg cgaaccgttg ctggtgtagg tgtggaagat ttcgccgaag
     3601 tacccgttgg tggtcaccag tgcggacccg tcgccgaaat cccaggtgac ggtcatgccc
     3661 gtcagatcgg ccgccacatc cgagacgctc caggtgaagc gttgcggcgt gccgcgcttg
     3721 gccgtggccg tggcgccgtt ctggggcgtc tgaaccacag gcgcgacatt ggccaccttg
     3781 acgatgcacg gcacgctttc gccgctgtac acagcgtcga tcgcggcatt ggtgatcacc
     3841 ggcatgatcg tgatgccgaa aagttcgctg gcgtggatgc cgtccggcac gttgaacgcg
     3901 accgtgtccg aattgtgatt gcccgcgcgc acgcggacaa acttgggcgt ctcgctgaac
     3961 gtcacgttat tgctttgcgg accgccgttg agcgtggtgt ccagccgcac ccaaacatct
     4021 tccgtataga cttgcgacaa ccgcacctgc agggcaccgg tgccgaaggc cgaggtttcg
     4081 ggcagggtct gctcattgtg cggcgcgggg gcgttcggca gcagaaccga gacatacggt
     4141 tccggggccg gggccaccac gatctgccgg gtctcggcgt tttggacgcc gaggctcgcg
     4201 ttgttgtcgt aatcctgcgt gccctgcagg aaaaccgtgt tggtgccgac cgtgaggccg
     4261 gtgatctgga aggtggcggt ggtcgaaccc ccggcgatca tcagcttttg ggatttaacc
     4321 ccgttcaggt tcacatcccc ttgcgacacg gtgttggaaa cccacacgta aaagctcacc
     4381 gtgcgtccgg acgaaaccgg attgacggtg gaaacggccc acgtcaccga ctcgccgacc
     4441 tgcaccggcg agggcgcgga actgaactgc agggttttga tccccacgga gcggtaactg
     4501 aaatcataat gcgtcggatc atcgccgggc agttcgagat atcggggcga cgtggtgcgg
     4561 aaacgccaga ccgcattgct ggccggatca ctcacgcgca cgatcttcca agtgtcattg
     4621 caccaatcga acccgtaggg cacaccgagc acatcgctgc cgtccgaacc cgagatcttg
     4681 agcttcgaga ccatgtcccc ttcgcggatc cggtacttga aggtgacgcg cgtcttctga
     4741 cccacgccat ccgtgaaccg ctggacatcc gccggatcct caagcgtggc ccacgcctcc
     4801 tcttccgccg ccagattcat gcgcaaccgg ggcatggcaa tcgggccgac cgccgtgttc
     4861 gtgagcatga aatggcccca cagacgcagt tcgatcggaa tcgcggtgcc aacgttgctg
     4921 agcgttttgt tcgtgatact caccgaggtg agcaccccga tcagatctcc gttcgagggc
     4981 ggaaagtact gggcccgggc tgtcacgcct gcccacaaaa ccccgcaaac cacccaaggc
     5041 aacaccgact ttttgattcc cgttttcata tccgttgttt tcatgcctcg tctcctgtcc
     5101 atgcgaaaaa catactctcc cgtccacacg ggttacggtg ccgcagtctc tcccgcggcc
     5161 ggccgggccg ttccctcggc caccctccgg ttgtcacgct cctcggccag catccgctgc
     5221 cggacccgct cccgggcctg agccagattg tcctgaatct tgccgatcca ctcctgattc
     5281 gcgttctcaa gccggttcca ctccggatcc ttcaccagca ccgccttgac cgcggcatcg
     5341 tcggctccct ccggcaacgt cgcgcgcgcg cgctccacca tcacccgcat ctgctcaacc
     5401 acctcggcgc gcccgcccgc aagccgcgtc tgctctctcc gcagctccgc aagctcggcg
     5461 cgatacgccg cgtccgccat ccggttcgtg atgacctccg gcagagcctc ctcggcgggc
     5521 ggccgacgct tcgaacaccc gttcaaaaaa aagagggaaa aaacgattga caggaatgcc
     5581 gttcctaagc ggaccgccac accacactct cggattttcc aaccgttaca ctcaaaagct
     5641 atccgcattc cactcgcttc ccggtccgtt caaaccgctg caaccatacc ctgtcacgcc
     5701 aagggcggac tatgccgcga ttgttatagc ttaccatgcc caaaagaggt cgggcaagcc
     5761 ctattaaaag aaaaacaggg ttgcctacat ccgtacgcat gcgttattat gaacgcacga
     5821 tgactcaaga ccggaccaac aatgtggccg ccgagactct ggtgctcgcg gacggggaga
     5881 accagcagag cctgctgcgt gtgcgccgta tcaaactgct cgtgatcagc gggccgctcc
     5941 agggacgcga aattctggtc aaccgcgaca ccttcaccat tggttcgggc agccacaatg
     6001 acctgatcgt tgaggattcg accgtctcca agcgccactg cgagattgtg gtggagcaga
     6061 gcggctacct catccgcgat ctggagagca ccaacggcac cctcgtgcag ggggtgcgcg
     6121 tctcctccgc ccacctccct ccggggtcgg agatccaact cggcaaaacc cgcatcgtct
     6181 tctgccccct tcaggaagcc cacgacatcc ccctctccaa tcgggaggcc ttcggcgcca
     6241 tgctcggcag ctcggtaccg atgcgccgga tcttctatct ggccgagacc tactcgccag
     6301 ccgacgtcac ggtcatgatc accggcgaga ccggcaccgg caaggagatt ctcgccgagg
     6361 agattcataa ccactccccg cgccacgaca agccgttcat cgtgatcgac tgcgccgcga
     6421 tctccaaaga gctgatcgag agcgagctgt tcggccatgt caagggcgcc ttcaccggtg
     6481 ccaacgcgga ccgccagggc gccttcgaac tggccgacgg cggcacggtc tttctggatg
     6541 agatcggcga cctctcgccc gacctccagc ccaagctgct gcgcgtgctc gaaa
//
LOCUS       DAOSRB010000155         6546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_10843_length_6546_cov_6.815129, whole genome shotgun sequence.
ACCESSION   DAOSRB010000155 DAOSRB010000000
VERSION     DAOSRB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10843_length_6546_cov_6.815129"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLZ74_08370"
     CDS             <1..532
                     /locus_tag="PLZ74_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOR98377.1"
                     /translation="TGDLSELALGWSTYNGDHMSMYGINAGVPKTLVRDLIRWVAEHR
                     ADASVAPVLSDILDTPVSPELLPADAAGSITQKTEDVLGPYEMHDFFLYQMVRCGASP
                     EKIRFLAAQAFEGRYGEEEISRALDLFLRRFFSQQFKRSCLPDGPKVGSINLSPRGDW
                     RMPSDASPESWLVKTH"
     gene            757..1978
                     /locus_tag="PLZ74_08375"
                     /pseudo
     CDS             757..1978
                     /locus_tag="PLZ74_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
     gene            2150..2554
                     /locus_tag="PLZ74_08380"
     CDS             2150..2554
                     /locus_tag="PLZ74_08380"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOR98378.1"
                     /translation="MAEIGRLVADLAGRLGIAAGSLSGLTLPAPVAKAAPAAVKPASA
                     PKSAKPEAPAVAGPTINAPLNGTFYRSPGPGKPELAKEGDSLQTGDSICIVEAMKLFN
                     QIKADKPLKVVKFLVSHGTVVAKGQPLVSVQY"
     gene            complement(2602..4371)
                     /locus_tag="PLZ74_08385"
     CDS             complement(2602..4371)
                     /locus_tag="PLZ74_08385"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOR98379.1"
                     /translation="MKRHHIAPVMIGMGLILVVGLSALPARTQTRDPSAPAQPVSETA
                     SGVLATGKKSVQAVGEATSELGDSLCSGGRQAVDQSRKLWQDVLLPAMQRFAAALPGV
                     LKAVILLLAFWLVGKFAGACVTRLLNLTRIDERAVRDWGLESLLKRPTGETCSLASLV
                     GNIVKWLLVLFGFVAFFNALNLSMVAGPLQNLLDRIIGVIPNLIKAVAILLVYWILAA
                     VVRAGVTRALGALKFDERAGKHLSLPTTDESQPLGPGAMIGRLLFYVILLFGIPPFLQ
                     ALDQQALVAPLQDMLSKALGILPNLFAAALILVVGRVVTAIVREVVTNFLAASGADAA
                     AEKFGLSRTLGGNRLSAIAAKVVSFFILIPILISAVDCLGIRAISDPLKAMLQTILDA
                     VPALLVAAIIVLIGYVVAKVVRALVESFLSGIGTDELPGRLGLTFLEARAGQTPPSKL
                     IGAITAVVILLITAQQACAKLRFDQLAELLQRIVAYLPNLAVGLIILLAAMSLGKRFG
                     TMVAGALENHANGKALSVVAHYAVLLLGLSMALEQLGVGKEIVMIAVGSLLGGSALAL
                     GLAFGLGGQDRARELIERWKTGK"
     gene            4676..5722
                     /locus_tag="PLZ74_08390"
     CDS             4676..5722
                     /locus_tag="PLZ74_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98380.1"
                     /translation="MSSPRITKEARASWLRLYETVDRIDRLSPWLWMEPASCFGVAVP
                     DMEEPCFVLFGEPGREMRAIRILLGWKAFYEMMTRLADPAKQGVAWLLEIRMIEVLCA
                     PEPLLFQHERDFLRAIRREPDGKGEWTIFRSIVPGYHPWLPDAKERKLLEDVLYQAFG
                     MALRVEADPGMLRSRFPREILIRTSDGKGGWRDVWSRVKELSGEEIEVRIESRRLQAL
                     AAKPTCPVTLQVDLLFTPFSIQPGRERPQTAYVLLAVDAQSGLIHCGELFQAIGGIAA
                     MWSEIPDRLIALFERLGGCPDAIEVRGDRMANLMRPLTEFLPFKLVRREHLKMLDEAR
                     VGLSAYLRNGEEEA"
     gene            complement(5685..6359)
                     /locus_tag="PLZ74_08395"
     CDS             complement(5685..6359)
                     /locus_tag="PLZ74_08395"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOR98381.1"
                     /translation="MPNLPYRTLSLLTLVLLAVILPFFLFADAVQAWTDALLRHAQAH
                     PLQTGLLLTALLASDIVVPVPSSLVSTACGMTLGFVAGTCASFIGMSASALVGLLMGR
                     MAAPAAQRLIGADEVRLLQSFHARYGIWLLLALRPVPVLAEASVVFSGLARQPFGRAF
                     AITLLGNAAVSAVYAAVGAWGRLADSFLPAFGASMLLAGALYLPLRRRGQHRATLPPP
                     RSADTR"
BASE COUNT         1294 a         1991 c         2102 g         1159 t
ORIGIN      
        1 caccggtgat ctttccgagc tggcattggg atggtccacc tacaatgggg accatatgag
       61 tatgtacggc atcaacgccg gcgtgcccaa aaccttggtg cgcgatttga tccgttgggt
      121 ggcggagcat cgggccgacg cgtcggttgc cccggtgctg tccgacattc tggacacccc
      181 cgtttcgccg gaactgctgc ccgccgatgc cgcgggaagc atcacgcaaa aaacggagga
      241 cgtcctgggc ccctacgaga tgcatgactt cttcctctac cagatggtgc gttgcggcgc
      301 atcgccggag aagatccgct ttctggccgc acaggcgttc gagggccggt acggggaaga
      361 ggagatcagc cgtgcgctgg atctgttcct gcggcgtttt ttcagccagc agttcaaacg
      421 ctcctgcctg ccggacgggc ccaaggtcgg gtccatcaac ctttcgccgc gtggtgactg
      481 gcgtatgccc agcgacgcct ccccggaaag ttggctcgtt aaaacacatt aacaatcttc
      541 ggggtgtggg gggggattcc cgaaaatcgt tcgtgacaaa atgacgggaa atgtcagaat
      601 gaaaggcatt tttctttcag aggagaaaag gtctctatga aacctgcgcg caagagcaag
      661 caaaacacgc ggagcgtaat ccctccggtc aagccccgca tccgcatcac ggacaccacg
      721 ctgcgtgacg cccaccagtc cctctgggcc acgcgcatgc gtacggatga cattctcgcg
      781 atcgccgagg aggtggacaa cgccgggtac tattcgctgg agtgctgggg cggggcgacg
      841 ttcgacgtgt gcctgcgttt tctgcgcgag aatccttggg agcgtctgcg ccagctgaag
      901 gcggtctgta aaaagacgcc gctgcagatg ctgctgcgcg gacagaatat tttgggatac
      961 aaaaactatc cggacgatgt gctggagcgg ttcatcgcgc tcgcatgcga gaacggcatg
     1021 gatatcttcc gcattttcga cgcgctgaat gacacgcgca atctcgaaaa agcgatccgc
     1081 accgtcaagc aatacggcgg gcacgcgcag ggtacgatct gttacaccta cagtccggtg
     1141 cacacggtcg aaaatttcgt ggccatcgcc aaagagcagg aggccatggg tatcgacacc
     1201 ctgtgcatca aggacatggc gggcatcctc actccggccg cggctacgga tctcatcggg
     1261 gcgctcgtca aagcggtcgg cgttccggtt caggtgcact catatgacca gcggcatggc
     1321 ggtcgccatg tatctctccg cagtcaaggc cggtgccggg gcgatcgaca cagcggtttc
     1381 gaccatgtcg gggttctcct ctcagccgcc gacagagacg ctgatggcga ttctggaggc
     1441 ggaaggttac gataccgggc ttgatcgcgc tttggtggcc aagagcaacg cccatttccg
     1501 gatgctgaag gcggtgcgtg aacccgcctc ctccggtcag atggaatcgg tggatgcgga
     1561 ggtgctggcg catcacatcc ccggcgggat gatctccaac ctgcgctcgc agttgcagca
     1621 gcagggcgcg ctggaccggt tgcccgaagt gttggaggag ttgccgcgga cgcgcaaaga
     1681 catggggtat ccgccgcttg tcacgccaac cagccagatt gtgggcgtgc aggctgtgtt
     1741 gaatgtgctc gccggaaaac gctatgagat ggtcacgcag gagaccaggg attacgtccg
     1801 gggcctttac ggccgttcgc ccgccccgat cgacacccgt ctggcaagca aaatcctgaa
     1861 cggcgagaag cgtgtgacgt gccgtcctgc ggatctgctc aaccccggct tggctgaagc
     1921 cgcggcgcag atcgatccgc gcctgatcga gaaggaggag gatatccttt cgtactgcct
     1981 cttccccgag gtggcgctgg gctacttcgc gtggcgcgcc gcgccgcctg atcagcgcga
     2041 cccgattccc gcagatctcg aggcttcgga taagaagcag accgcgtcgg ccgcggttcc
     2101 ggaagcggag cccgatcccg tgtccgcgcc tgcggaggag actctggcca tggccgagat
     2161 cggcaggttg gtggcggatc tcgccgggcg gctgggaatc gccgctggga gtttgtccgg
     2221 cctgaccctt cccgcgccgg ttgcgaaagc cgcgcctgcc gcggtcaaac ccgcgtccgc
     2281 gccgaagtcc gcgaaacccg aagcgcccgc cgttgcggga ccgacaatca acgcgccgct
     2341 gaacggaacg ttttaccgct cgccgggtcc gggaaaaccc gaactcgcca aagagggcga
     2401 ttcgctccag accggtgatt cgatctgcat tgtcgaggcc atgaagctct tcaaccagat
     2461 taaagccgac aaaccgttga aggtggtcaa gtttctggtg tcgcacggta cggtggtggc
     2521 caaaggccag ccgttggtgt ccgtccaata ctgatccgct tcggacaaaa aaagggcggg
     2581 gggttccccg ccctttttcc attactttcc ggttttccag cgttcgatca gttcgcgcgc
     2641 ccggtcttgg cccccgagtc cgaaggccag tcccagagcg agcgcactgc cgcccagcag
     2701 ggatcccact gcgatcatca cgatctcctt gcccacgccg agttgctcca gcgccatgct
     2761 gaggccgagg aggagcacgg cgtagtgcgc gacgacggag agcgccttgc cgttcgcgtg
     2821 gttctccagc gcgcccgcca ccatggtgcc gaaacgcttg ccgaggctca tggccgcaag
     2881 caggatgatc agccccaccg ccagattcgg caggtaggcg acgatccgct gcaacagttc
     2941 ggccaactga tcgaaacgca acttggcaca ggcctgctgc gccgtgatga gcagaatcac
     3001 caccgccgtg atcgcgccga tcagcttaga gggcggtgtc tggccggccc gcgcctccag
     3061 aaaggtcagc cccaaccgcc cgggcagttc gtccgtaccg atcccgctga ggaaggattc
     3121 caccagcgcc cggaccacct tggccacgac atacccgatc aacacgatga tggccgcgac
     3181 cagcagggcc ggcacggcat cgagaatcgt ctggagcatg gccttgagcg gatccgagat
     3241 cgcacggatc cccaggcagt ctaccgcgga gatgaggatc gggatcagga tgaagaacga
     3301 aaccaccttg gcggctatgg cggagagacg gttcccgccc agcgtccggc tcagaccgaa
     3361 cttctccgcc gcggcatccg cgccgctggc ggcgaggaag ttggtgacca cctcgcgcac
     3421 gatcgcggtc acgacgcgtc ccaccaccag aatcagggct gcggcaaaga gattgggcag
     3481 gataccgagg gctttggaga gcatgtcctg caacggcgcc accaaggcct gctggtcgag
     3541 cgcctgcaaa aacggcggaa tgccgaagag caggatgacg tagaagagca accggccgat
     3601 catcgcgccg gggccaagcg gttgactttc gtccgtagtc ggcagagaca ggtgtttgcc
     3661 agcccgctcg tcgaacttca gtgcccccag agcgcgtgtg accccggccc gtaccacagc
     3721 ggccagaatc cagtagacca gcaggatggc gaccgccttg atcaggttgg ggatcacgcc
     3781 gatgatgcgg tcaagcaggt tctgtaacgg ccctgccacc atcgagaggt tcagcgcgtt
     3841 gaaaaacgcg acgaacccga acagcaccaa cagccacttc acgatgttgc ccacaaggct
     3901 ggcgagcgaa caggtctcgc cggtcggccg tttcagaaga ctttcgagtc cccaatcgcg
     3961 cacggcacgt tcatcaatcc gggttagatt cagcagccgg gtgacgcagg cacccgcgaa
     4021 cttgccgacc agccagaagg ccaatagcaa aatcacagcc ttgagcacac cgggcagggc
     4081 ggcggcaaac cgctgcatcg cgggcaacag cacgtcctgc catagcttgc ggctttgatc
     4141 aaccgcctgc cggcccccgc tacacagcga gtctcccagt tccgacgtgg cttcgcccac
     4201 ggcttgaaca ctcttctttc cggtcgccaa aacgcccgac gcggtttccg aaaccggttg
     4261 tgccggcgcg gaggggtctc gcgtttgggt acgggctggg agggcgctca gccccacgac
     4321 cagaatcaat cccattccga tcatcacagg ggcaatatgg tgtcgtttca taggagggct
     4381 cctttccgag ttacggtttg tgagggtcag gcatccgttc ttaccggact cctccggtcg
     4441 catgccgtcc gtcaccatta ggttaatatt tttcagctgc gcatgcaatc aaaatgcgta
     4501 tcgtaatcgg ttcaccgcga agcgcaaatc agggccttgt ggtttctgtg gctctttgag
     4561 gaaaaatccg ctactgtacg cgacaaagag gcggaaaaga aaatggcgcg ccgacccgtt
     4621 gggcaagatg cgggccgcgc ggtttttgaa gcggttttca acaacacagg tggtcatgtc
     4681 ctccccacgc ataacgaaag aagcgcgcgc gagttggctg cggctctatg agacggttga
     4741 tcggatcgat cggttgtcgc cgtggctgtg gatggagccc gcgtcgtgtt tcggcgtggc
     4801 cgtgccggat atggaagagc cctgctttgt gttgttcggc gagccgggac gggagatgcg
     4861 cgccatccgg attctgctgg ggtggaaggc gttttacgag atgatgaccc gtctcgccga
     4921 tcccgcgaag cagggcgtgg cctggctgct ggaaatccgc atgatcgagg tgctttgcgc
     4981 gccagaacca ttgctgttcc aacacgagcg ggactttctg cgcgcgattc ggcgcgaacc
     5041 ggatgggaaa ggcgagtgga caatcttccg ctccatcgtg ccgggctatc acccgtggct
     5101 tccggatgca aaggaacgca agttgctgga ggatgtgctg tatcaggcgt tcggcatggc
     5161 cctgcgggtc gaagccgatc ccggcatgtt gcgcagtcgt ttcccgcgcg agatcctcat
     5221 ccggacatcg gacgggaagg gcgggtggcg ggatgtgtgg tcacgggtca aagaactgtc
     5281 gggcgaggag atcgaggtcc gcattgaatc ccggcggttg caggcgctgg ccgctaagcc
     5341 gacatgcccg gtcaccttgc aggtggatct gctgttcacc ccgttttcga tccagcctgg
     5401 ccgcgagcgc ccgcagaccg cgtatgtgct gctggccgtg gatgcgcaga gcgggctgat
     5461 tcattgcggt gagttgtttc aggcgatcgg agggatcgct gccatgtggt cagagattcc
     5521 cgaccggctg atcgcgttgt tcgagcgctt gggcggatgt cccgatgcga tcgaggtgcg
     5581 gggggaccgc atggcgaacc tcatgcgccc gctcaccgag tttctgccgt tcaaactggt
     5641 gcggcgcgaa cacctgaaga tgctggatga ggcgcgcgtg gggcttagcg cgtatctgcg
     5701 gaacggggag gaggaagcgt agcgcggtgc tgcccgcgcc gccgcagcgg caggtacagc
     5761 gcaccggcca gcagcatcga ggccccgaaa gcgggcagga acgaatcggc gaggcgaccc
     5821 cacgcgccta cggcggcgta aacggccgag accgccgcgt tgcccagcag cgtgatggcg
     5881 aaggcccgtc cgaaaggttg acgcgccagg ccgctgaaca cgaccgaggc ctcggccaga
     5941 accggcacgg ggcgcagcgc cagcagcagc cagatcccgt accgtgcgtg gaaggattga
     6001 agcagccgga cctcgtctgc tccgatcaga cgctgcgccg cgggtgccgc catccgcccc
     6061 atcagcaggc cgaccagcgc gctggcgctc atgccgatga acgaggcgca ggttccggca
     6121 acgaaaccca gtgtcatgcc gcaagcggtg ctgaccagac tggagggcac gggtaccacg
     6181 atatctgatg ccagcagcgc ggtcagcagc agaccggttt gcagcgggtg ggcctgcgca
     6241 tggcggagca gcgcgtcagt ccaggcctga accgcatccg cgaacaggaa aaacggcagg
     6301 atcaccgcca agagcacgag cgtcagcaac gacagggttc ggtaaggcag atttggcatc
     6361 accccgtaac catactaaac cgacatgccg tgcgcaacca tcacgcaagg attttgggca
     6421 cagccatgac tttcgtgtgt tctgtgtgtc ccgtggtgaa gcgatcccga tcccgatagc
     6481 gatcccgata gcgatagcga tcccgatccc gatagcgatt tggatgaaac cttgaaaccc
     6541 agaaac
//
LOCUS       DAOSRB010000156         6483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_10983_length_6483_cov_7.651369, whole genome shotgun sequence.
ACCESSION   DAOSRB010000156 DAOSRB010000000
VERSION     DAOSRB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10983_length_6483_cov_7.651369"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..2904
                     /locus_tag="PLZ74_08400"
     CDS             268..2904
                     /locus_tag="PLZ74_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR98382.1"
                     /translation="MEGVREAPILTDPVSAAACPKCGAMLDVGGLPTFTAVQCPNCET
                     EFQVPAQFGNFLLLQLLGAGGMGGVYRARDEGLNREVAIKVMLKSLGDDPMFVETFQR
                     EAQAAAKLNHPNIAQIYSFGQEKGQPYIVMELVSGGSLDKMMAAQGAMDPTAVIQIGE
                     QIAEGLREAADAGLVHGDVKPENILFDNDKNAKLVDFGLAAMNAGPGNEVWGTPYYIA
                     PEKVRRQKSDFRSDIYSLGATLYHAITGVPPFDGEDATAVVKARFEGPPKPMSELRKN
                     IPDEIEALIRRMLEVEPQMRYPTYGSLLGDMRRYLSKVKPVNLALSSKKIMIKGKLSK
                     TTTGVPTITGNMSDDLPPGMVPVDAIPEEDLESEEEAKRRGHRMMLMILGGVFLGVAM
                     LVGSVFGVKHYLGVKREKTVRAINESRQGKALASIAKSVAQARTLPERLSGYPQEAME
                     TAQAAADAVVAVLGETVRTSMTVPEPTFAWDAQESADAQPPSAPGETAAAPAVSNAVP
                     QGGAAEVTNTVAAAGTNLVTEAGTNASVTAAAPAAPAAPAPEAVPAAGSDDPTAPREG
                     DAHPVVAIVRGMFAEAYAVKGGVLLAEHLVKKIEAEAKAAEGMTDLEQTEALVKHANA
                     LVEKVNGLAYVKEVADVPRKVSQLKKTLESVKTDTASLAEMKRREAQEQEKRRKEEEI
                     AEKKRQEKEAHQKKVEEEKAKVADAELANRDMLKALQFREAVNAIRELRGGLETDEGG
                     IAASIAIDRINRMRDFHNYLIAQVKGFKSARGWSVDAADAKTLSVGGIKIPWADVYNK
                     RPEIIAELINGLVRSEQATKTLKLSEKTRLMTSAALCLNLFYGEMASAQELAKQIALA
                     AAQQFEIEADTIKQLLPGFFTE"
     gene            2906..4288
                     /gene="thrC"
                     /locus_tag="PLZ74_08405"
     CDS             2906..4288
                     /gene="thrC"
                     /locus_tag="PLZ74_08405"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOR98383.1"
                     /translation="MRYISTRGGGEPLTFRQTLLTGLARDGGLFLPETFPDVRDRLNA
                     WRTLPYPELAFQIIRLFADDIPEDALRRIVEQSYAGFRHHEVCPVVPVGPVRMLELFH
                     GPTLAFKDVALQFLGNLFEYALAETGAEMNIVAATSGDTGSAAIYGVRGRERIRIFVM
                     HPAGRVSPVQERQMTTVLDANVHNLAVEGTFDDCQNMVKSLFGDLAFRDTFRLGAVNS
                     INWARVLAQIVYYFYAAFRTQEATGAAAVRFSVPTGNFGDIFAGYVAYRMGLPVDQLV
                     LATNENDILARYFNTGVYSLGEVAPTLSPSMDIQVASNFERYLYCLAGGDAAMLRGWM
                     GAFKAEGTLRPDLPPDPVIVAGRGDTEMTLTTIRAFWETHGYLLDPHTAVGVAVGMRF
                     MHPEIPLICLATAHPAKFGDAILRATGQDLAHHPILDALATLPTRVRTVPADVDAVAK
                     IVAATVRGDG"
     gene            4285..4620
                     /locus_tag="PLZ74_08410"
     CDS             4285..4620
                     /locus_tag="PLZ74_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOR98384.1"
                     /translation="MIDPATFRCRQCGACCRVPGYVALAEGETEAIAAFLGMDVYAFT
                     SRYTTLTFNRGTLSLTEREDGRCVFLEDDNTCLIQPVKPAQCKGFPFLWSTPALEHVC
                     AAIRPARDS"
     gene            complement(4688..6340)
                     /locus_tag="PLZ74_08415"
     CDS             complement(4688..6340)
                     /locus_tag="PLZ74_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR98385.1"
                     /translation="MRKRFLCGSILLLFASQLIGQETAAEGKVWDLVVYGGTSGGIAA
                     AVQARRMALDVVVIEPTQRIGGLTTGGLGQTDIGNKVAVGGIAREFYRAVRAWYAQPA
                     AWIWQKREQYRDGGQTRTDQNEDAMWTFEPSVALKIYEAWVRTNAIPVVRGERLDRAS
                     GVALTRTVPWRILSIRMESGRVFRGKMFIDTTYEGDLMAAAKVSYTVGRESNAQYGED
                     YNGVQAKIDLKYKHHNFLPGVDPYVEKGNAASGLLPFIDPTGPGKEGAGDKRVQAYCY
                     RMCLTDHPENRIPFVKPEGYDPLWFELLLRNFEAGRQGIWINSDMPNRKTDTNNYGGF
                     STDFIGQNYDYPEASYAEREKIAARHLLYQQGLMWTLANHPRVPEHVRKEVSRWGMCR
                     DEFTEGGGWQEQLYVREARRMVSDLVMTQHHCQGKVRAEAPVGLAAYGMDSHNVQRYI
                     DAQGYVQNEGDVELGWLKPYPVGYFAIVPKRKECANLLVPVCVSATHIAFGSIRMEPV
                     FMILGQSAATAAALAIQDDVAVQEVDLAKLQTRLLADGQRLK"
BASE COUNT         1323 a         1946 c         2050 g         1164 t
ORIGIN      
        1 aggtctgacg ggagaagcgg gcgtccctac cccgcgtggc agggcggttt ccccgaagcc
       61 gaggacgaag ccgagaaagc gatcccgata aaaccagaaa tcaaagcctc agcgatccgt
      121 gactcgggga tacgttcagc gtgctgggtt tgaaggtttt tcggcatcga caacgccctc
      181 ttctttcggc aaactagaat gctttccggt tgacatggta aaaaacagga atggcacacg
      241 gatgagcgaa caagcgaggg tggacagatg gaaggtgtga gagaagctcc gattttgacg
      301 gatccggtga gtgcggcggc atgtccgaaa tgcggcgcga tgctggatgt cgggggatta
      361 ccgacgttta ccgcagttca gtgcccgaac tgcgagacgg aattccaggt gcctgcgcag
      421 ttcggtaatt tcctgctcct gcaactgctg ggcgcgggcg gcatgggcgg tgtgtaccgt
      481 gcccgggacg agggcctcaa ccgcgaggtg gccatcaagg tgatgttgaa gagcctggga
      541 gatgacccca tgttcgtgga gacgtttcag cgtgaggcgc aggccgcggc caaactgaac
      601 catccgaaca tcgcgcagat ctattcgttc ggtcaggaaa aagggcaacc gtatattgtg
      661 atggaactgg tttccggcgg cagcctcgat aagatgatgg cggcacaggg agcgatggac
      721 cccacggccg tcatacagat cggcgagcag atcgccgagg ggttgcggga ggccgcagac
      781 gccggactgg tgcacggcga tgtgaagccc gagaatatcc tgttcgacaa cgacaagaac
      841 gccaaactgg tggatttcgg tctggcggcc atgaatgccg gaccgggtaa tgaagtttgg
      901 ggtacgccgt actacatcgc gccggaaaag gtacggcggc agaaaagcga tttccggtcc
      961 gatatttaca gtctcggcgc aacgttgtat cacgcgatca ccggggtgcc gccgttcgac
     1021 ggcgaggatg ccacggccgt ggtcaaagcg cgtttcgaag ggccgcccaa accgatgtcg
     1081 gaactccgta aaaacatccc ggacgaaatc gaggcgctca tccggcgtat gctggaggtc
     1141 gaaccccaga tgcggtatcc gacctacggg tcgctcctcg gtgatatgcg gcgttacctt
     1201 tccaaggtga aaccggtcaa tctcgcgttg tcatcgaaaa aaatcatgat caagggcaag
     1261 ttgtccaaga ccaccaccgg agtgccgacg ataacgggaa acatgtcgga cgacctgcct
     1321 cccggcatgg tgccggtgga cgcgattccg gaggaggatc tcgaaagcga ggaagaggcg
     1381 aagcggcgcg gccacaggat gatgctgatg atcctcggcg gagtgtttct gggcgtggcg
     1441 atgctggttg gaagcgtctt cggcgttaaa cactatttgg gggtaaaacg ggaaaagacc
     1501 gtgcgcgcga taaacgaaag ccgtcaaggc aaggcgctgg cttcgatcgc gaaatccgtg
     1561 gcacaggcga gaacgttgcc ggaacggttg agcggatatc cgcaggaggc gatggaaaca
     1621 gcccaggcgg cagccgacgc ggtggtcgcg gtgttgggcg agacggtgcg gacgagcatg
     1681 accgtgcccg agccgacgtt cgcctgggat gcgcaagaga gcgcagacgc gcaaccgcca
     1741 tccgcaccgg gcgaaacggc tgcggccccc gcagtctcga atgcggtgcc gcaaggcgga
     1801 gcggcggagg tcaccaacac cgttgccgcg gcagggacca accttgtgac agaagcgggg
     1861 accaatgcgt ccgtcacggc tgcggcgccc gcggcgcccg cggcacccgc gccggaagcc
     1921 gtgccagcag cgggaagcga tgatccgacc gcgccgcggg agggcgacgc gcatccggtg
     1981 gtggccatcg tgcggggcat gttcgcggag gcgtacgccg tgaaaggcgg ggtgctgctc
     2041 gctgaacatt tggtaaagaa aatcgaggcc gaggcgaaag cggcggaggg catgaccgac
     2101 ttggagcaga cggaagcgct ggtcaagcac gcgaatgcgc tggtcgagaa ggtgaacggc
     2161 ttggcgtacg tcaaagaggt ggcggatgtg ccgcgtaaag tctcgcaact gaagaagacc
     2221 ctcgagagcg tcaagaccga caccgcctct ctggcggaga tgaaacggcg ggaggcgcag
     2281 gaacaggaga aacggcgcaa ggaggaagag atcgccgaaa agaagcgtca agagaaagaa
     2341 gcccatcaga aaaaggtgga agaggagaag gccaaagtgg cggacgcgga actcgcgaac
     2401 cgcgatatgc tcaaggcctt gcagttccgc gaggccgtga atgcaatccg ggagttgcgt
     2461 ggtgggctgg agaccgacga gggaggaatc gcggccagta tcgccatcga ccggatcaac
     2521 cgcatgcggg atttccataa ttacctgatc gctcaagtca aaggcttcaa atccgcgcgc
     2581 ggctggtcag tcgacgcggc ggacgcgaag accctctcgg tgggcggcat aaaaatcccg
     2641 tgggctgacg tttacaacaa acgcccggag atcatcgccg aactgatcaa cggcctggtg
     2701 cggagcgaac aggcgaccaa gaccctgaag ctgagcgaga aaacccggtt gatgaccagt
     2761 gcggcgttgt gcctcaatct gttttacggg gagatggctt cggctcagga actggccaag
     2821 cagatcgcgc tggcagcggc tcagcagttt gagatcgagg ccgacacgat caaacagctc
     2881 ctccccgggt tcttcacgga gtgacatgcg gtacatcagc acccgaggcg gtggcgagcc
     2941 gctgacattc aggcagaccc tgctgaccgg tttggcgcgt gacggcggac tgttcctgcc
     3001 cgaaaccttc ccggacgtgc gcgaccgcct gaatgcgtgg cgcacgctgc cctatcccga
     3061 attggccttt cagatcatcc gccttttcgc ggacgacatt ccggaggatg cgctgcgccg
     3121 tatcgtggaa caaagctacg cggggttccg ccaccatgag gtctgccccg tggtgccggt
     3181 gggaccggta cgcatgctgg agctgttcca cggaccgact ctcgcgttca aggatgtggc
     3241 cctgcagttc ctcggcaacc tcttcgagta cgcgctggcc gagaccggcg cggagatgaa
     3301 catcgtggcc gcgaccagcg gcgataccgg gagcgccgcc atctacggag tacgcggacg
     3361 tgaacggatc cgcatcttcg tgatgcaccc ggccggccgc gtgagcccgg tgcaggaacg
     3421 gcagatgacc accgtgctcg acgcgaacgt gcacaacctc gcggtggaag gcacgtttga
     3481 cgactgccag aacatggtca agtcgctgtt cggcgatttg gcattccgcg acacgttccg
     3541 gctgggcgcg gtgaactcga tcaactgggc ccgcgtgctc gcccaaatcg tctactattt
     3601 ctatgcggcc ttccgcacac aggaggctac cggtgccgca gcggtgcgtt tcagcgtgcc
     3661 gaccggtaat ttcggcgata tcttcgcagg gtatgtggcg taccggatgg gactcccggt
     3721 ggatcagctg gtgttggcca cgaacgagaa cgacattctc gcgcgctatt tcaacacggg
     3781 agtgtacagc ctgggcgagg tcgcgccgac cctgagcccc tccatggaca ttcaggtggc
     3841 cagcaacttc gagcgttatt tgtactgtct ggcgggcggg gacgcagcca tgctgagagg
     3901 atggatgggc gccttcaagg ccgaggggac tctgcgcccc gatttgccgc cagaccccgt
     3961 gatcgttgcc ggacgcggcg acacagagat gacgctgacc accatccgcg ccttctggga
     4021 gacccacggg tacctgctcg atccgcatac ggcggtgggt gttgcggtcg gcatgcgttt
     4081 catgcatccg gagatcccgc tgatctgcct ggccacggcg catccggcga agttcggcga
     4141 cgcgatcctg cgcgcgaccg gacaagatct ggcgcatcat ccgatcctcg acgcgctggc
     4201 cacgctgccc acgcgcgtgc gcaccgtgcc ggccgacgtg gacgcggtgg caaagatcgt
     4261 cgcggcgact gtccggggag acggatgatc gatcccgcca catttcgttg ccgtcagtgt
     4321 ggggcgtgtt gccgcgtacc cggttacgtc gcgctggccg agggcgagac agaggcaatc
     4381 gccgcgtttc tcggaatgga cgtgtacgcc ttcacctccc gctacacaac gctgaccttc
     4441 aaccgcggga ccctctcgct cacggaacgc gaagacggac ggtgtgtgtt tctcgaagac
     4501 gacaacacct gcctcatcca gccggtcaag cccgcacagt gcaaagggtt ccccttcctc
     4561 tggagcaccc ccgcgctgga acacgtctgc gcggccatac gacccgcacg cgattcctga
     4621 tccggacgtt atttccggga gcccccgaac agctcgtccg gcacggcggg cgcaggctcc
     4681 ggcaggccta cttcaatcgc tgtccgtcgg ccaacaggcg cgtctggagt ttggcgaggt
     4741 ccacctcctg caccgcgaca tcgtcctgaa tcgccaacgc cgcggctgtg gcagccgact
     4801 gtcccaagat catgaagacc ggctccatgc ggatcgagcc aaaggcgatg tgggtcgcag
     4861 agacgcaaac cggcacgagc aggttggcgc actccttacg tttgggaacg atcgcgaagt
     4921 acccgaccgg atagggtttg agccacccga gttccacatc accctcattc tgaacatatc
     4981 cttgggcgtc gatgtaccgc tgcacgttgt gggagtccat gccgtatgcg gccaatccga
     5041 cgggcgcttc ggcacggacc ttcccctggc aatggtgctg cgtcatcacg agatcgctca
     5101 ccatgcgccg cgcctcccgg acataaagct gctcctgcca tccccctccc tccgtgaact
     5161 cgtcccggca catgccccag cgcgacacct ctttccgaac gtgttccgga acacggggat
     5221 ggttagccag cgtccacatc agcccttgct ggtacaggag gtgacgggcg gcgatcttct
     5281 cgcgttcggc ataggaggct tcgggataat cgtagttctg cccgatgaag tccgtggaaa
     5341 acccgccgta gttgttggtg tcggttttgc ggttgggcat atccgaattg atccagatcc
     5401 cttgtcgccc cgcctcgaag ttccgcaaca acaactcgaa ccacaaggga tcataccctt
     5461 ccggtttgac gaagggaatg cggttttccg gatggtcggt cagacacatg cggtagcagt
     5521 aggcctgaac gcgtttgtcc cccgcaccct ccttgccggg ccccgtcgga tcgatgaacg
     5581 gcagcagccc gctcgcggca ttgcccttct cgacataagg atcgactccg ggaagaaagt
     5641 tgtggtgctt gtatttgaga tcgatcttgg cctgcacacc attgtagtcc tcgccgtact
     5701 gcgcattact ctcgcggccg accgtataac tgaccttcgc cgctgccatc agatcgccct
     5761 cataggtcgt gtcgataaac atctttcccc gaaacacccg cccgctttcc atgcggatcg
     5821 agaggatccg ccacggaacc gtgcgcgtca gggccacgcc cgacgcacgg tcgagacgtt
     5881 ccccccggac aaccggaatg gcattcgtgc ggacccaagc ttcgtaaatc ttgagggcca
     5941 cgctcggctc aaaggtccac atcgcgtctt cgttttgatc cgttcgcgtc tggcctccgt
     6001 cccggtactg ttcccgcttc tgccagatcc aagcggccgg ctgggcgtac caggcacgga
     6061 ccgcgcggta aaattcacgc gcgatgccgc cgaccgcgac cttgttgccg atatcggtct
     6121 gccccaaccc gcccgtggtc aacccgccaa tacgttgcgt cggttcgatg accaccacat
     6181 ccaaagccat gcgccgggcc tgcaccgcgg ccgcgatccc gcccgacgtg ccgccgtaga
     6241 cgaccagatc ccagaccttc ccttccgctg ccgtctcctg cccgatcagt tgtgacgcaa
     6301 acaacaaaag gatgcttccg cataaaaaac gttttctcat gatgctccct tcttgatcta
     6361 tcattccatt aaaaaacgca ttgtccctgt tcgggggcgc ttcgtcaacc ttgattttgg
     6421 cctttcgcgg aacaagttta ggacatgatt aacatgatgt tgttcaatcc tgaaaatcct
     6481 gtc
//
LOCUS       DAOSRB010000157         6464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11028_length_6464_cov_6.998284, whole genome shotgun sequence.
ACCESSION   DAOSRB010000157 DAOSRB010000000
VERSION     DAOSRB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11028_length_6464_cov_6.998284"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..2333)
                     /locus_tag="PLZ74_08420"
     CDS             complement(117..2333)
                     /locus_tag="PLZ74_08420"
                     /inference="COORDINATES: protein motif:HMM:NF015612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,3-alpha-glucanase family
                     glycosylhydrolase"
                     /protein_id="HOR98386.1"
                     /translation="MKKWIIAVALAAVNGFAGTNPERKVYAHFMGCWPVFGCGGQDKS
                     MKAFLTTGEAVECGPVGGRFNNFPLVPQEGFNMKLLDKAKLEIKRAIRGGIDGFAFDA
                     WAGDQSPQTLDVYFQAAEELKVDFGLTICFDPSCHGAKWIEGTNMCEKYATSAKWVLR
                     HLDSPNLARFHGKPLFFGYYSKGIVKDSGSFLTAEKRREIKAAWDAWRKALPCEVYLH
                     GSIEGYVDWKNFERNDYPAIAKDCAETFDAVGAFTGWDANWANRNDLWKHIRDAGCGW
                     SQPIVPQYCNKRGGIITGAGLDVLHQCWTNAIDRGSELLQFVTWNDYGEETILGPAYG
                     SNYTFLRVNRHYADWLKQGKPPVIEKDEVHVVFRRCCDPQKTTFPFLDRRINLPTALE
                     VITFLTKPGKVVVEGYGKYEAPAGMFSKKFDEHPGNVSVKVLRRNWLFLNQTVCQLTA
                     PEEISAKKWREDDVAATWSSTYDEEWALDFPGIEPLYYSENGDLDGDGLPNWFEMVYF
                     GEFPYMKTASGADPNADPDGDGFTNLQEYENETNPLIPDTPYASGFVWSLQELAERAL
                     IGNPARDSKGRYVWRREYQYGAKGHDYVPGSAFKEILSGGGSLQSQMTHVYAPNPFGG
                     GGFDCGIQFNKPEKGQAEARVHRDSPIALTWIAPVACTVDVVTKVTPKNPKVKARTTL
                     VLGDKVLGTTVARKIQVGKGDEIRLVIENLNPWSGGDLFAIEKFDVIWAKESERAN"
     gene            3454..4842
                     /locus_tag="PLZ74_08425"
     CDS             3454..4842
                     /locus_tag="PLZ74_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOR98387.1"
                     /translation="MRKRWLCAGLTVLAMPVLAQDGAAATEKKAELDFDAGMDIRARY
                     EFKDNWMNKGKTSVNPAYEDYYRLRTRFWGKATFGDDYAAFLRLANEFRDYRNSTGNK
                     RKNEFPDELFIDNLYLDFNNLADRVDLRVGRQDIREGAGRVISDGTPGDGSRSTYFDA
                     LFAKVRFLEKSDVDLMATWNRYRDDWTLGNPHDVYDNTKIKSGSPYSKMDEKGLMAYV
                     HYNEIENFPMEFYWIWKQEDRFFDKKTRYPGRDFHTLGTRLMPKINEKLSAEVEAAVQ
                     TGQIDSQSGMRGRDILAWMTYAGLTYSEKEVFSKPKLTGAVLWMSGDTDSYYKTQDGS
                     TDTGWNPVFNRTTWFSELCSSMYDQYRWSNLIYPHLEAAVEPFQKHKVKVQCGPMFAH
                     EKDNGATSSYRGLYTQARYDFPLLSKIFGKRGEVKGAVVGEALWYGDYYQHDAVNEDV
                     ATWLRFELNGKF"
     gene            4937..5749
                     /locus_tag="PLZ74_08430"
     CDS             4937..5749
                     /locus_tag="PLZ74_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOR98388.1"
                     /translation="MKGSLLVAGLVACCAVAQAQDKLTLDGSTTVGPIAKAFAEFYMR
                     QNPGVNITVSESGSGNGAKALLNGTCDIANLSRNLKDAERKAMEEKGVKPVEHTVAFD
                     ALPVIVHPSNRVKGLTLEQVRDIYTGKITNWKEVGGADQRIVVISRDTNSGTYETFKD
                     LVLGKDNKILESAEYTGSNGGVRQRVQMTRGAIGYAGLGFVDRTVKALEINGVAPCAE
                     SVVQKRYPISRALFMFTNGEPAADTLAAKFIGLSKTARGKEIIEEIGFVPVP"
     gene            5798..>6464
                     /gene="pstC"
                     /locus_tag="PLZ74_08435"
     CDS             5798..>6464
                     /gene="pstC"
                     /locus_tag="PLZ74_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOR98389.1"
                     /translation="MRRKTIGFLGRSFLFAVTSVAAVAVFAIILFIARDALPFFRDRT
                     SLEFFVSKSWYPSHTPPQFGAAAIFFGSFLVTAAAVILAVPSGVMAAICLSDILPFKA
                     RQLVKPVIEILAAIPSVVYGFFALTVVAPFLQNAGEWLPGNIEIASGVNALNASVILA
                     IMALPTIVSVSEDALQAAGRELREGSYALGATRSETLLKVVIPAAFSGIFSAVLLGIM
                     RAMG"
BASE COUNT         1367 a         1830 c         1883 g         1384 t
ORIGIN      
        1 gagccatttt actacaaacg cccgaccaga catctggccg ggcgtttgtt tttttcgctg
       61 gctccatgca gaatcgccgc ctggatcact tgatcttaat cgccgtatgg gctaattcaa
      121 ttagcccttt ctgattcctt cgcccagatg acatcgaact tctcgatcgc gaacaaatcg
      181 ccgccactcc agggattgag gttctcgatc acgagccgga tttcatcccc tttcccgacc
      241 tggatcttac gcgcgaccgt agtcccaagc accttgtctc ccagaaccag cgtcgtcctc
      301 gcctttacct tcggattctt cggcgtcacc ttggtcacga catcgaccgt gcaggcgaca
      361 ggcgcgatcc aagtaagcgc aatcggtgaa tcgcggtgaa cgcgtgcctc ggcttgtccc
      421 ttctcaggct tgttgaactg gatcccgcag tcgaaaccgc cgccgccgaa cggattcggg
      481 gcatagacat gcgtcatctg tgactgcaac gagcccccgc ccgagagaat ctccttgaat
      541 gcactgcccg gcacgtagtc gtgtcctttc gcaccgtact ggtactcgcg tcgccacacg
      601 tagcgcccct tcgaatccct cgcaggatta ccgatgagcg cacgctccgc gagttcctga
      661 agcgaccaca caaatcctga cgcatagggg gtatcgggaa tcagcgggtt cgtctcgttc
      721 tcgtactcct gtaggttggt gaacccgtca ccgtccggat cggcattcgg atccgcaccg
      781 gacgccgtct tcatatacgg gaactccccg aaataaacca tttcgaacca gttgggcagg
      841 ccatccccgt cgagatcgcc gttctccgaa tagtacagcg gttcgatgcc gggaaaatcc
      901 aacgcccact cctcgtcata ggttgaactc catgtggcgg caacatcatc ttcacgccac
      961 ttcttggcac tgatttcctc gggggccgtc aattgacaga ccgtctgatt gaggaaaagc
     1021 cagttgcgcc gaagaacctt cacggacacg tttccgggat gctcgtcgaa cttcttcgag
     1081 aacattccgg ccggggcttc gtatttgccg tagccctcga caacgacctt gcccggcttg
     1141 gtcaggaagg tgatcacctc aagcgcggtc ggcagattta tacggcgatc aaggaacggg
     1201 aacgtcgttt tttgcggatc gcagcaccgg cgaaaaacaa catgaacctc atccttctcg
     1261 ataacgggcg gcttcccctg tttcaaccag tcggcgtaat ggcggttcac gcggagaaac
     1321 gtgtaattcg aaccatacgc gggaccgagg atcgtctctt cgccgtagtc gttccaagtc
     1381 acaaactgca acagttccga accgcggtca atcgcgtttg tccaacactg gtgcagaaca
     1441 tcgagcccgg cacccgtgat gatgccgccc ctcttgttgc agtattgcgg cacaatcggc
     1501 tgactccagc cgcatccagc gtcccggata tgtttccaga gatcgttgcg gttcgcccag
     1561 ttcgcgtccc acccggtgaa ggcgccgacc gcatcaaagg tctcggcgca gtcctttgcg
     1621 atcgccggat aatcgttgcg ctcgaaattt ttccagtcga cgtatccttc gatcgagccg
     1681 tgcagataga cctcgcacgg cagcgccttg cgccacgcgt cccaggctgc cttgatttca
     1741 cggcgctttt cggcagtcag gaaggatccg ctatccttga cgatgccctt tgaataatag
     1801 ccgaagaaaa gcggtttgcc gtggaagcgc gcgaggttcg gggaatcgag gtgacggagc
     1861 acccacttcg cggacgtggc gtacttttca cacatattcg tgccttcgat ccactttgcg
     1921 ccatggcagg agggatcgaa acagatggtg agtccgaaat cgactttcag ttcttcagcc
     1981 gcctgaaaat agacatcgag cgtctgcggg gactggtcgc ctgcccaagc atcgaacgcg
     2041 aatccgtcga taccgccgcg gatcgcgcgt ttgatctcca gcttcgcctt gtcgaggagc
     2101 ttcatgttga acccctcctg cggcaccagc gggaaattgt tgaagcgtcc gccgaccggt
     2161 ccacattcca cggcctcgcc ggtcgtcaga aacgccttca tggacttgtc ctggcctccg
     2221 caaccgaaaa ccggccagca ccccatgaaa tgggcataca ccttccgttc gggattcgtc
     2281 ccggcaaagc cattcaccgc cgcgagcgca accgcgatga tccacttttt catgtctttc
     2341 ccttttccat cgctgtaccc acttggagtt tcaccacgtt gccgcatctg ctccgactac
     2401 ggacagcgtg atcgctgtac aaaacgataa cggactgacc tttacggtca tcgtaatccc
     2461 ttattggatg taaatcacgg aaccgacctt ctttggtgcg gtatagacaa ggctcttgcc
     2521 gctgttttca atcgacagcg ttcccggatg actggaactg aaccgactgt tgccgatcag
     2581 ctcctgaaac gcagccgcgg aatctctcga ggtctcgatc aaaacaagcc tcccattcgg
     2641 atgctcgcgg tcaaaatcac gggcggcgat ctccagtttg ctttcgtcat ggacactttc
     2701 tcatcaaagc gacattttat cactagcatc ccatagggta aaccagtaac ccgccagccc
     2761 ttatgaattg gtatcatgtt atgtgcgggt gggtaaaagt caacccttaa aaattctcaa
     2821 aaaatccgtg ttcggaatga tccagcggcg gcttgccgac gcgtacatgc tcgacatccg
     2881 caagtggtca ggaggtgcgg gctccgcccg cgttggggcg gaatcgggat cggaatcgct
     2941 atcgggatcg tccgcggacg cctcggggat gcgtccctac ccggcgtggt agggcggttt
     3001 ccccgtaaac cgccgccggt ccctgccgga cgggcgtccc gtgatcagct actacgacgc
     3061 cacccgcggc atgttgaaac tggccgagtg ggtcgaaggc atccggcgtc cgtgatgcgg
     3121 cgcctcgaca ggggataagc gtatccgcag gggcggcggg gtcactgaac gatcaagtgc
     3181 ccgccgctcc cgttcccgca tcctcgggcg tccgagacgg ccgcccgcac tttgcattgc
     3241 ccttccgatc catgccggta cggcgggacg tccacagctg cgcgccatcg gcgcggagcc
     3301 ggaccgccga aatcaatttt gatccgatat cgtttcaaca tatatttctc atgtactcct
     3361 caccgaaatc ttattaacat tcggcatgat gacgccgttc caagaggaaa taggcgaaaa
     3421 caacacctct gcgtgaacag aaaaggagca gacatgcgaa aacgatggtt atgtgcaggg
     3481 ttgacggtct tggcgatgcc tgtgttggcg caggacgggg cggcagcaac ggagaaaaag
     3541 gcagaactgg atttcgatgc gggaatggac atacgtgccc gttacgagtt caaagacaac
     3601 tggatgaaca agggcaagac atccgtcaat ccggcgtatg aggattatta ccggctccgg
     3661 acgcggtttt ggggaaaggc tacgttcggc gatgattacg cagcttttct tcggctcgcc
     3721 aacgaattcc gcgattaccg caacagcacg gggaataaac ggaagaacga gttccccgac
     3781 gagcttttca tcgataacct ctatctggat ttcaacaatc tggcggaccg ggttgatctg
     3841 cgtgtcggac ggcaggatat ccgggagggt gcgggccggg tgatcagcga cggaacgccg
     3901 ggtgacggtt cgcgctccac gtactttgac gcgctctttg ccaaggtgcg ttttctggag
     3961 aagagcgatg tggatttgat ggccacttgg aaccggtatc gcgatgactg gactctgggc
     4021 aatccgcatg acgtctatga caacaccaag atcaaaagcg gcagcccgta ctccaagatg
     4081 gatgaaaagg gcttgatggc gtatgtgcac tacaacgaaa tcgagaactt cccgatggag
     4141 ttttactgga tttggaaaca ggaagaccgg tttttcgata agaaaacaag ataccccggc
     4201 cgtgattttc acacgctcgg aacgcgtctg atgccgaaga tcaatgagaa actgtcggct
     4261 gaggtcgaag cggctgtcca gacagggcag attgacagtc agtccggtat gcggggacgc
     4321 gacatcttgg cgtggatgac ctatgcgggc ctgacctact cggagaaaga ggttttcagc
     4381 aaaccgaagc tgaccggcgc ggtgctctgg atgtccggtg acacggatag ttactacaag
     4441 acgcaggacg gcagcacgga cacgggatgg aacccggttt tcaaccgcac gacctggttc
     4501 agcgagctgt gctcgagcat gtatgaccag taccgctggt ccaacctgat ctacccgcat
     4561 cttgaagccg ctgttgagcc gttccaaaaa cacaaggtca aagtccagtg cgggccgatg
     4621 ttcgcgcacg agaaggacaa cggcgcgaca agctcgtatc gcgggcttta cacgcaggcg
     4681 cgatacgact tcccgctgct gagcaagatc ttcgggaaac gcggggaagt gaaaggcgct
     4741 gtggtcggtg aggccttgtg gtacggcgac tactaccagc acgatgcggt caacgaagat
     4801 gtggcgacat ggctgcggtt cgaactgaac ggcaagttct aacggttttc taacaaaggt
     4861 gacacgtttg tctaacggga atagggttca ctcacatcga acggagaggt tgtctgttca
     4921 caacacagga gtaaaaatga agggttcatt actggtggca gggttggtgg cttgttgcgc
     4981 ggttgcgcag gctcaggata agctgacgct ggacggctcg acgactgtcg gaccgatcgc
     5041 caaggcgttc gcggagtttt atatgcggca gaatccgggc gtgaatatta cggtcagcga
     5101 atccggtagc ggcaacgggg ccaaggctct gttgaacggg acatgtgaca tcgccaatct
     5161 ctcgcggaat ctgaaggatg cggagcggaa ggcgatggaa gaaaagggcg tcaagcccgt
     5221 ggaacacacg gtcgcatttg acgcgttgcc ggtgatcgtg catccttcca accgggtgaa
     5281 gggcctgacc ctcgagcagg tgcgggatat ctataccggc aagatcacca actggaaaga
     5341 ggtgggcggc gcggatcagc gtattgtggt gatcagccgc gacaccaaca gcggcaccta
     5401 cgaaaccttc aaggacttgg ttctcggcaa ggacaacaag atcttggaga gtgccgagta
     5461 caccggcagc aacggcgggg tgcgtcagcg ggtgcaaatg acccggggcg cgatcgggta
     5521 cgccggactc ggattcgtgg accgtacggt caaggcgctg gagatcaacg gtgtcgcgcc
     5581 gtgcgcggaa tcggtggtgc agaaacgcta tccgatctcg cgggcgcttt tcatgttcac
     5641 caacggcgaa ccggcggcgg acacgttggc cgcgaaattc atcgggttga gcaaaaccgc
     5701 ccgcgggaaa gaaatcatcg aggagatcgg gttcgttccg gttccatagg acatgagggc
     5761 taccaccgga tcacttcaga tgtcttccgg cgcaataatg cgccggaaga ctatcggttt
     5821 tttagggcgt tcctttctgt tcgccgtcac gtcagtggcg gcggtggccg tgttcgcgat
     5881 cattctattc atcgcgcggg atgccctgcc ctttttccgc gatcggacct cgctggaatt
     5941 ttttgtctcc aaatcgtggt atccgagtca cacgccgccg cagttcggcg cggctgcgat
     6001 ctttttcggc agttttctgg tgacggcggc tgcggttatc ttggccgtgc cgtccggcgt
     6061 gatggctgcc atctgcctca gcgacatcct gccgtttaag gcgcggcagc tcgtcaagcc
     6121 ggttatcgag attctggccg cgattccctc cgtggtctac gggtttttcg ctttaacggt
     6181 cgtggcgccg tttttgcaga atgcgggcga gtggttgccg ggcaacatcg aaatcgcgag
     6241 tggcgttaat gcgctgaacg catctgtgat cctcgcgatc atggcgttgc cgacgattgt
     6301 cagcgtctct gaagatgccc tgcaggcggc gggcagggag ttgcgcgagg ggagttacgc
     6361 gttgggtgcg acccggagcg agacgttgct caaagtggtg atcccggcgg cgttcagcgg
     6421 aatcttctcg gcggtcttgc tggggatcat gcgcgcgatg ggcg
//
LOCUS       DAOSRB010000158         6397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11157_length_6397_cov_7.855093, whole genome shotgun sequence.
ACCESSION   DAOSRB010000158 DAOSRB010000000
VERSION     DAOSRB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11157_length_6397_cov_7.855093"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..2528
                     /locus_tag="PLZ74_08440"
     CDS             384..2528
                     /locus_tag="PLZ74_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98390.1"
                     /translation="MGQTWGRVIPLSISLLLGGCSGMGVAQKGTVLHVAPDGNDVWSG
                     RLERAAKGDGPLATLDGARRAVRALRTSGTLPPGPVTVLFAPGVYRQTAPLFFTEQDA
                     GTAASPVRYVARPGTEVIFSGGIVISPDWEVRDDAAAARLDAAARPHVRIYDIGNGRL
                     PEIAEPGQKGWFAPLWISENGIPLTAARWPNTGFVKIADATRSKKGSAPSPEANGFTY
                     AGDRPDRWVGEADPRVSGFLGNDWAAANVRVASIDPALKRITQGKPSSAYGFRKGGRW
                     FGYNLLCELDAPGEVYADLKAERLFVWPKTDTKNAEREVARCRALLILRETAHLTFEG
                     ITFTSALESGVEVRRSEDLGFTRCQFRSIAGTGLLMESVRRVRVAGCEFTHLGAGGLS
                     ASSGDAQQLTPGGLVVDNNHFAFFGLTSLSYAPALQLSGCGARVTHNLFHEGPHAAIL
                     FGGREHRIAYNEIHSVCLVAGEMGAIYTGRDWTLVGNVIENNHIHDIYRACPQPTRGI
                     MLDDGVGGLTVRNNLFVRCAEGLCLAAIGNVVSNNMFVACFPAIGTWRNWTAPEEFDL
                     SIRNNGTRLARLAKVPVDASPWKERYPELAMLRDAIRDRKPRPAATYSRIVANVAWKG
                     AGEWIHNHYKTFDGWVIENNLVTEDPLFVSPERDDYRLRTQSPAHKKGIRPLPTERMG
                     LYASSERAVWPVSHPVNTGCGNLTYVPSSDKN"
     gene            complement(2541..3962)
                     /locus_tag="PLZ74_08445"
     CDS             complement(2541..3962)
                     /locus_tag="PLZ74_08445"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOR98391.1"
                     /translation="MKKHFDLSLRPVGRHVSAGGCAGLRGGLTLAGLLFAGTLSAWGP
                     HPDITMNAVAAIGPDAPLARALGTETAQLRNTCWIPDVWMQLSGTYYFDDYLFTPERP
                     THPHTSHSHSFLCENGKYGQHSKVLFSHYFRRALQALRTENAQNAARWVGALLHFTED
                     TGAPPHAILEHGPLHGPMENWLNGKQISIAGYVPRVLGNDDASAFEAYWANQERLNNE
                     TIEIANRIKPLVAAGDRAAAEPLILMCANASARNAADLCATLGALLGTDSGGGVLQGR
                     VTSETVPGPLGGVPAKVMLAGTSYSTLADAKGVYRFRNLPPGTYRLLAVRPGSELFEG
                     PVTVSGAETVYDIVMPPARVSGNLLRNPAFALRWTSPDHRDMWYQRGRDRIWRTEAVP
                     VKPGQNCRVHLEWKRPGTEAVLRFTTHEREGKRLAEHALAHPDSRLEAVAPDKATHAH
                     IEVKGCAGLPDAELAYVALTVEK"
     gene            3972..4655
                     /locus_tag="PLZ74_08450"
     CDS             3972..4655
                     /locus_tag="PLZ74_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98392.1"
                     /translation="MRISLGAVKPRNRWLRIRPQIALHFLPLLFVKFPFHGFIQIGIG
                     IGIAIGIDCLTTGHFLWFQGFWFQGFMVSRFHPNRDRGRDRYRDRSLNHAHGMALLGF
                     LSTDLHRLHRLFWGIARVFLERQGKFLIFMKFHGFQGFIQIAIGIGIAIGIDYLTTNS
                     RDGTARVFIHRFTQSTQIFWGIARVFLERQGKFLIFMMFYVFHGFIQIGIAIGIGIGI
                     DHYKLWPGE"
     gene            complement(4656..5693)
                     /gene="hisC"
                     /locus_tag="PLZ74_08455"
     CDS             complement(4656..5693)
                     /gene="hisC"
                     /locus_tag="PLZ74_08455"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOR98393.1"
                     /translation="MYIRQSIAALQAYTPGEQPRDPGIIKLNTNENPYPPSPRVTEAL
                     AAFDCSRLRLYPDPLFHALRQRLAEIHGCSDRQIFIGNGSDEILALSTCAFVENHGSI
                     GYFTPSYSLYPVLTEIRNVEKRPVELTADFRWRMPEAYQASLFLMTNPNAPTSMLFDK
                     PVVTAFCRAFNGVVLVDEAYGDFADTSCMGLALEAGQTNTLVLRTLSKSFSLAGLRFG
                     YAVGPEALIAALYKIKDSYNLDMISQTLGLAALNDLPHMHENVRRIRATRERLTIELR
                     GRGWRVCDSQTNFLFARPPDGNAKQVFDLLKAAKILVRYFPGPATGEYLRITIGTDDQ
                     MDTLLDRLAEM"
     gene            complement(5842..>6397)
                     /locus_tag="PLZ74_08460"
     CDS             complement(5842..>6397)
                     /locus_tag="PLZ74_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98394.1"
                     /translation="ANAGWIAFEETGDPRVNLVTGDLSGYVWGANLGWISLGGVRTTV
                     LSAGPDSDGDGIPDPWEMRETGGLTALAGGAHDADGDGVCDVDEYGADTDPLDSQSML
                     AFTAFSRSSTTNRLTWTVEQTRFYELWRSPTLSPHDWSPTGLGVMAPGAGATMTREVD
                     TAPPVGPPPATQFYRVKAVIPLGE"
BASE COUNT         1150 a         1897 c         2058 g         1292 t
ORIGIN      
        1 tataaaaaaa taaacccggg tgcttatgcc gggcagtcct ttccagaacg tcacgtcaca
       61 ttcgcgtttt attggcccgc gacgagcggc ttgcggtgtc gggaacgcgg gtgtcctcgc
      121 ccgtaacggc ggatcagccg gatcggaggc gtccccgttg caggtcgagt gtcgcgggtc
      181 gcaagtcaac gtacacggga acggggacgt gaacgggcac accgatcctg ataccggaat
      241 ccccggcttg agcccggcgg acgtgttgtg taagatcggt ggttgtcatg actcgtgttc
      301 tgttccccct tcattcgggt acggcgcatc acggttgcgc ggctggcggc ggaaaggaat
      361 gcggtcatcg gggaggagat cgcatgggtc aaacttgggg gcgcgttatt ccgctatcga
      421 tcagtctgtt gctgggcggc tgttccggca tgggcgtggc acagaagggg accgtgctgc
      481 acgtcgcgcc ggacggaaac gatgtctgga gcggacggct ggaacgggcc gcaaagggtg
      541 acggcccctt ggcgacgctg gatggcgcgc gtcgggcggt gcgtgccttg cggacatccg
      601 gcaccttgcc gccgggaccg gtgacggttc ttttcgcgcc gggcgtttat cggcaaaccg
      661 cgccgctttt tttcacagaa caggacgcgg gtaccgcggc atcgccggtc cggtatgtgg
      721 cgcggcccgg cacggaagtg attttttctg gcggcatcgt gatctcgccg gactgggaag
      781 tccgggacga tgcggcggcg gcccggttgg atgcggcggc gcggccccat gtccggatct
      841 atgacattgg aaacggtcgc ctgccggaga tcgccgagcc gggccagaag ggttggttcg
      901 ccccgctctg gatatcggaa aacggaatcc cgctgactgc cgcccgctgg cccaatacgg
      961 gattcgtgaa aatcgcggac gccacgcgtt cgaaaaaggg atcggcccct tcgccggaag
     1021 cgaacgggtt cacctatgcg ggtgaccgcc cggaccggtg ggttggggag gctgatccgc
     1081 gcgtctccgg ttttctcggc aacgactggg ctgcggcgaa tgtgcgcgtc gcctcgatcg
     1141 atcccgctct caagcggatc acgcagggca agccttccag tgcgtacggt ttccggaaag
     1201 gaggccgctg gttcgggtac aatcttttgt gcgagttgga cgcgccgggg gaggtttatg
     1261 ccgatctgaa ggctgaacgc ctttttgtgt ggccgaagac ggacacgaaa aatgcggaac
     1321 gtgaggttgc cagatgccgg gcgcttctga ttctgcggga aaccgcccat ctcacgttcg
     1381 aagggatcac cttcacctct gcgttggagt ccggtgttga agtcaggcgg agtgaggatc
     1441 tgggtttcac gcgctgtcaa ttccgctcca tcgcggggac ggggctgctc atggagtccg
     1501 tgcgccgtgt gcgggtggcc ggatgcgagt tcacccattt gggggcgggc ggtctctccg
     1561 cctcctcggg cgacgcccag caattgacgc ccggcgggct ggtcgtcgat aacaatcact
     1621 ttgcgttttt cggactcacc tctctcagct atgcgcccgc cctccagctc agcggctgcg
     1681 gcgcacgggt gacgcacaac ctgtttcatg aaggccccca tgcggcgatc ctgttcgggg
     1741 gacgcgaaca ccgcatcgca tacaacgaaa tccacagcgt ctgtctggtg gcaggggaaa
     1801 tgggtgcgat ctataccgga cgagactgga ccctggtcgg caacgtgatc gagaacaacc
     1861 atatccacga tatctaccgt gcttgtccgc aaccgacccg gggtatcatg ttggatgacg
     1921 gtgtgggcgg tttgaccgta cggaacaatc ttttcgtgcg ttgcgccgag ggactttgcc
     1981 tggccgcgat cggtaacgtg gtttccaaca acatgttcgt cgcgtgtttt ccggcgatcg
     2041 gcacctggcg gaactggacc gctccggagg agtttgacct ctccatccgg aacaatggca
     2101 cgcggctcgc gcggttggcg aaagtgccgg tggacgcgtc gccatggaag gagcgctatc
     2161 cggaattggc catgcttcgc gacgccatcc gggatcggaa accgcgtccc gccgcgacct
     2221 acagccggat tgtcgccaac gtggcttgga agggcgcggg cgagtggatc cacaaccact
     2281 acaagacgtt tgacggatgg gtcatcgaaa acaacttggt aacggaagat ccgctgtttg
     2341 tctctcccga gcgggacgat taccgtctcc ggacccaatc gcccgcacac aaaaaaggga
     2401 tccgtcctct gcccaccgaa agaatgggac tgtatgccag ctccgaacgg gcggtgtggc
     2461 cggtttcgca tccggtcaac accggatgcg gcaacctgac gtatgttccg tcatccgaca
     2521 aaaactgaaa tgccgccgtc tcacttttcc acggtcagcg ccacataggc cagctcggcg
     2581 tccggcagac ccgcgcaccc tttcacctcg atatgtgcgt gggtcgcctt gtccggcgcc
     2641 acggcttcga gccgcgagtc cggatgggct aacgcatgct ctgcaagccg tttcccctcg
     2701 cgctcatgcg tggtgaagcg cagcaccgct tcggtgccgg gacgtttcca ttcgagatgg
     2761 acgcggcagt tctgacccgg cttgacgggc accgcctcgg tgcgccagat ccggtcccgt
     2821 ccgcgctggt accacatgtc ccggtggtcg ggcgaggtcc accgcaaggc gaacgccgga
     2881 ttgcgcaaca ggttcccgct gacccgcgcg ggcggcatca cgatgtcgta cacggtctcc
     2941 gcgcccgaga ccgtgaccgg cccttcgaac aactcgctgc ccggacgcac tgccagcagg
     3001 cggtaggtgc cgggagggag attgcggaac cggtatacgc ccttagcgtc cgccagggtc
     3061 gaatacgagg tgccggcgag catcactttg gccggcacgc cgcccaacgg gcccggcacg
     3121 gtctcgctgg tgacgcggcc ctgcagcaca ccgccgccgg aatcggtgcc gagcagggct
     3181 ccaagcgtgg cgcacagatc agcggcgttc cgggccgaag cgttggcgca catgaggatc
     3241 agcggttcgg cggccgcgcg atccccagcc gcgaccaacg gtttgatgcg gttggcgatt
     3301 tcgatggttt cgttgttcaa ccgttcctga ttcgcccagt aggcctcgaa agcggaagcg
     3361 tcatcgttcc ccagcacgcg ggggacatat cctgcaatgc tgatctgttt gccgttcagc
     3421 cagttttcca tcggcccgtg cagcgggccg tgttcgagga tcgcgtgggg tggcgcaccg
     3481 gtatcctctg tgaaatgcag cagcgctcct acccagcgcg cggcgttctg cgcgttctcc
     3541 gtgcgcagtg cctgtaacgc ccggcggaag tagtggctga acaacacctt gctgtgctgg
     3601 ccgtacttac cgttctcgca aagaaaggag tgcgagtgcg atgtgtgagg atgcgtcgga
     3661 cgctcaggcg tgaacaaata atcgtcaaaa taataggttc cgctgagctg catccaaaca
     3721 tctggaatcc agcatgtgtt gcggagttgc gccgtttccg ttcccagtgc ccgcgccagc
     3781 ggcgcatcag gcccgatggc tgccaccgca ttcatggtga tgtccggatg ggggccccac
     3841 gcggacagtg tccccgcaaa cagcaatccg gccagcgtga gccctccccg taaccctgcg
     3901 caaccgcctg ctgaaacgtg ccgcccgacc gggcgtaacg acaaatcgaa atgttttttc
     3961 atgttgacag catgcggatt tcgctcgggg ctgtcaagcc gcgtaatcgc tggttacgaa
     4021 tccggccaca aatcgcattg cactttttgc ccctgctttt cgtgaagttt ccgtttcatg
     4081 gtttcatcca aatcggtatc ggtatcggga tcgctatcgg aatcgattgt ttaaccacgg
     4141 gacactttct ttggtttcag ggtttctggt ttcagggttt catggtttca aggtttcatc
     4201 caaatcggga tcggggtcgg gatcgctatc gggatcgatc acttaaccac gctcacggga
     4261 tggcactgct cgggttttta tccacagatt tacacagatt acacagactt ttttggggca
     4321 tcgctcgggt ttttttggaa cgtcagggga aattcctgat tttcatgaag tttcatgggt
     4381 ttcagggttt catccaaatc gctatcggga tcggtatcgc tatcgggatc gattatttaa
     4441 ccacgaactc acgggatggc actgctcggg tttttatcca cagatttaca cagagtacgc
     4501 agattttttg gggcatcgct cgggtttttt tggaacgcca ggggaaattc ctgattttca
     4561 tgatgtttta tgtgtttcat ggtttcatcc aaatcgggat cgctatcggt atcggtatcg
     4621 gtatcgatca ttataaattg tggccggggg agtaatcaca tctcggcgag ccggtcgagc
     4681 agtgtgtcca tctggtcatc cgtaccgatg gtgatccgga gatactcgcc ggtcgcaggt
     4741 ccggggaaat aacgcaccaa gatcttcgct gccttgagca gatcaaagac ctgtttcgca
     4801 ttgccgtccg gcggacgcgc gaagaggaag ttggtctgcg aatcacagac gcgccaaccg
     4861 cggccgcgca attcaatcgt caggcgttcg cgcgtcgcgc ggattctccg gacattctcg
     4921 tgcatgtggg gcagatcgtt cagcgcggcc agtcccagag tctgggagat catgtccaga
     4981 ttgtaggagt ctttgatttt gtacagcgcg gcgatcagcg cttccggacc cacggcgtac
     5041 ccgaaccgga gcccggctag cgaaaaggat ttggagagcg tgcgcaagac cagcgtgttg
     5101 gtctggcccg cctccagagc gagacccatg caggaggtgt cggcgaaatc gccgtacgcc
     5161 tcgtcaacca acaccacacc gttgaacgcg cggcagaatg cggtcaccac cggtttgtcg
     5221 aacagcatgc tggtgggcgc gttcggattg gtcatcagaa acagcgaagc ctggtaagcc
     5281 tccggcatgc gccagcggaa atccgctgtc agttccaccg gacgtttttc cacattgcgg
     5341 atctccgtca gtaccggata gagcgagtac gagggcgtga agtagccgat gctgccgtgg
     5401 ttttccacaa aggcgcaggt gctgagcgcg aggatctcat ccgagccgtt accgatgaag
     5461 atctggcggt ccgaacagcc gtggatctcc gcgagacgct gacgcaacgc atggaagagc
     5521 ggatcagggt agaggcgcag ccgggaacag tcgaacgcgg ccagagcctc ggtcacacgg
     5581 ggactgggag gataggggtt ctcgttggtg ttgagtttaa tgatccccgg atcgcgcggt
     5641 tgctcaccgg gggtataggc ctgcagggcc gcaatgctct gacggatgta catgatcgga
     5701 tagtgtatca gattccgtgc gtccggcaac cgtcgcgaac cgcaaacgct ccctgccggt
     5761 gcggcgggac gttcacagct atgcgccatc ggtgcggagc cggaccgccg aaaccgccgc
     5821 ctcacccagc ccttttttag gttactcccc cagcgggatc acggccttga cgcggtagaa
     5881 ctgcgtggcg ggcggcgggc cgacgggtgg agcggtgtcg acctcgcgcg tcatggtggc
     5941 gcccgcgccg ggagccatca cgcccaagcc cgtcggcgac cagtcatggg gcgacagcgt
     6001 cggcgaccgc cacagctcgt agaagcgggt ctgctcaaca gtccacgtca ggcggttggt
     6061 cgtgctggac cgcgagaacg cggtgaaggc cagcatcgac tggctgtcca gcgggtcggt
     6121 gtcggcgccg tattcgtcaa cgtcgcacac gccgtcgccg tccgcgtcgt gcgccccgcc
     6181 cgccagggcg gtgaggccgc cggtctcccg catctcccac gggtcgggga tgccgtcgcc
     6241 gtcgctgtcc ggtcccgcgc tcagcaccgt cgtgcggacg ccgccgaggc tgatccagcc
     6301 gaggttcgcg ccccacacat agccgctcag gtcgccggtt acgagattca cccgcgggtc
     6361 gcccgtctcc tcgaaggcga tccagcccgc gttcgcg
//
LOCUS       DAOSRB010000159         6393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11164_length_6393_cov_6.228779, whole genome shotgun sequence.
ACCESSION   DAOSRB010000159 DAOSRB010000000
VERSION     DAOSRB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11164_length_6393_cov_6.228779"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1338
                     /locus_tag="PLZ74_08465"
     CDS             76..1338
                     /locus_tag="PLZ74_08465"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOR98395.1"
                     /translation="MGEWKRVSLKDAGITLIDCDHRTPPASDNGYPYIAIPQLKGGRL
                     DLSDVRRISPAHYAEWTRKAKPKHHDVILSRRCNPGETAYVPADLECALGQNLVLLRA
                     DGTKVFPPFLRWLVRGPDWWEQINTFLNVGAIFDSLKCADIPNFKLPLPPLPEQKRIA
                     HILGTLDDKIELSQRMNATLEAMVWALFKSWFVDFDPVRAKIDGRKPEGMDAETAALF
                     PSEFQESPLGPIPKGWATGTLGDICLNPRRGVKPNEIEPQTPYIALEHMPRRSIALSE
                     WNESANVASGKLSFRKGEILFGKLRPYFHKVGVAPVNGVCSTDILVLSPKSLEWLGLV
                     LCHTSSDELIQFTDLASTGTKMPRTSWNDISSFKVVLPPRSIATAFTRIIQPMLNHIH
                     ANIHQTRTLATLRDTLLPSLLNETNGSV"
     gene            1346..2368
                     /locus_tag="PLZ74_08470"
     CDS             1346..2368
                     /locus_tag="PLZ74_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOR98396.1"
                     /translation="MKSVKVFIVKNLNSKTVMSALIGAIAGILIAVISIIPSCSKTAR
                     ENERLKERIACLEKDLEHSRTQASELQNELIPFKTVAYQRYGSMDTEAMRKLADALLS
                     VQKNLEEINAKTDQIKLVPDGSIKIGGAVLGEPEKLKEPFTNACNAYNSQRFEEAYVC
                     ATNYLAVYEESKLQDEGALFRMTRFNIPVSTALMFAFAAEGSMSKGNYKLALQQIESA
                     IALRKEPREPYFDAIKTALLMHLDQFKLANEMLFFYKSRPEEDRHFYYSSLLIWGYLS
                     ELNFPEGYEAIKKEFNLNFDIRTPMAFHTSAQTKDGKPMEAYALMLWTGLGTFKPINY
                     VPKDKK"
     gene            2396..5569
                     /locus_tag="PLZ74_08475"
     CDS             2396..5569
                     /locus_tag="PLZ74_08475"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOR98397.1"
                     /translation="MSNLTESILEDAALTWFKELGYTVAFGPHIAPDEPAAERDSFGD
                     TVLTGRLRDAVARLNPDIPAEAQEDAIRKVLRVATPSLVQTNRIFHRMLRNGVEVEYP
                     RPDGSIAGDRVRLVDFETPSENDWLAVNQFAVVEGPCSRRADITVFVNGIPLGLIELK
                     NAADADATIWSAYQQVQTYKAEIPSLLGYNAALVVSDGVSARIGSLTASQEWFKVWRT
                     IDGEGDAPKTALELEVLVRGVFDRRRFLDLIQHFTVFEEDPDTGAVNKIIAGYHQFHA
                     VNAAVEETLRASGMGVWGEAHDPKDTEGRYWTGRMNDGAAGDRRAGVVWHTQGSGKSL
                     SMLFYAGRIIRNREMQNPTLIVLTDRNDLDDQLFGQFQRCHEILNQTPVQAESRAHLS
                     ELLNRSSGGVIFTTIQKFLPEKGERMPALSGRRNIVVIADEAHRSQYDLIDGLARHMR
                     DTLPFATFIGFTGTPIEKADANTRAVFGDYISVYDIQRAVADKATVPIYYESRIAKLA
                     LNAAAVPKLDEAFEEITEGEELTRKEKLKTKWAALETLVGDPKRIALVAQDIVTHFER
                     RWEAMDGKAMVVCMSRRICVELYNAIIRLRPDWAGAEDDDVDAERGKDCVVKVVMTGS
                     ADDGPEWQPHIRNTLKRRKLAVRFKDSKDSFRIVIVRDMWLTGFDAPCLHTMYLDKPM
                     QGHSLMQAIARVNRVFRDKPGGLVVDYLGLADQLRKALAVYTESGGMGDPTYDTAQAI
                     AVMLEKHEVACSMLHGFKWAKWTTGTATERLQLIPAGQEHILAQADGKQRFVKAVTEL
                     SRAFALCAASDEARAIRDDVSFFQTLQAALNKQSLGSRKTPEQLDAAVRQLVSKAIVT
                     EGQVIDVFTAAGIAKPDISILSDQFLAEVRGLKHKNVAAELLEKLLRDELRIRSRRNI
                     VQAELFSERLKKALNAYHNRAISTLEFIDELIRMAKDLDAASKRGQDLGLNDDEVAFY
                     DALATNDAAVKVMGDDKLKVIAVKLLSTVRKSVTIDWNLRESARAKIRVMVRRILREY
                     GYPPDLQEEAVKTVLAQAELVCADWES"
     gene            5726..5962
                     /locus_tag="PLZ74_08480"
     CDS             5726..5962
                     /locus_tag="PLZ74_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98398.1"
                     /translation="MKFVDRLTAHLQTATEPTEGWRFHDLPGVLLRLYEESQAARDSE
                     CVGACLDAWDRLFEAGFERVRDLSNELSSYLPQS"
     gene            6053..6259
                     /locus_tag="PLZ74_08485"
     CDS             6053..6259
                     /locus_tag="PLZ74_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HOR98399.1"
                     /translation="MKNEIIFNVTQDADGGYTAEALGEDIFTQADTWEELRANVREAV
                     AAYTFDRLEKPSVRIRLTREEVLA"
BASE COUNT         1493 a         1852 c         1781 g         1267 t
ORIGIN      
        1 ccaagcccgc cgagcgcgaa accatgctca acctcgtcat gaccttcctt gcggaggatg
       61 gtcaaggagg cgggcatggg tgagtggaaa cgggtcagcc ttaaggatgc agggatcaca
      121 ttgatcgact gtgaccatcg cacacctcct gcttcagata acggctatcc atacatcgcc
      181 attccacaac tcaagggagg ccgcctcgac ctctctgatg ttcgccgcat atcacccgct
      241 cactatgcgg aatggacacg aaaggctaaa ccaaaacacc acgacgtgat tttatcccgc
      301 cgttgcaacc cgggtgaaac cgcctatgtt cctgctgatc tagaatgtgc tctcggtcag
      361 aatcttgtct tacttcgcgc tgacggtacc aaggtctttc ccccgttcct gcgttggctc
      421 gtgagaggcc cggattggtg ggagcagatc aacaccttcc tcaacgtagg ggctatcttt
      481 gacagtttga agtgtgcaga cataccgaat ttcaagttgc ctcttcctcc cctccccgag
      541 cagaagcgca tcgcccacat cctcggcacg ctggacgaca agatcgagtt gagccagcgc
      601 atgaacgcaa cgttggaagc gatggtgtgg gcgctgttca agtcctggtt cgtggacttc
      661 gaccccgtcc gcgccaagat cgacggacgc aagcccgagg gcatggacgc agaaaccgcc
      721 gcgctcttcc cttccgagtt tcaagagtcg ccgctggggc cgattccgaa ggggtgggcc
      781 actggtacgc tcggtgacat ctgtttgaat cctagacgag gtgttaagcc gaatgaaata
      841 gaaccccaaa cgccctacat tgccttggaa cacatgccgc gccgcagtat tgcactcagc
      901 gaatggaatg aaagtgccaa cgtggctagc ggtaagttgt ctttccgcaa aggcgaaatc
      961 ctattcggaa aattgcgtcc atactttcac aaagttggag tcgcccccgt gaatggagtg
     1021 tgttcgaccg acatccttgt gctttcgccg aaaagcctag aatggttggg gctcgttctt
     1081 tgccatacga gtagtgatga attgatccaa tttaccgacc tcgcctcaac cggaactaaa
     1141 atgccacgaa cgagttggaa cgacatcagt tccttcaaag tcgtattgcc gccgcgctct
     1201 attgctactg cttttacacg catcatccaa ccgatgctca accacatcca cgcaaacatc
     1261 caccaaaccc gcaccctcgc caccctgcgc gacacgctgt tgccgagctt actgaatgaa
     1321 acgaacggat cagtgtaggt aatgtatgaa atcagttaaa gtgtttatcg taaaaaatct
     1381 aaatagcaaa accgttatga gtgctctcat cggtgcgata gctggtattc tcattgccgt
     1441 gatcagtatt attccatcgt gttctaaaac ggcaagagaa aatgagaggt tgaaagaacg
     1501 catagcctgt cttgagaaag atctcgaaca ttctcgtact caagcgtcag aactccagaa
     1561 cgagttaata ccgttcaaga ccgtcgcata ccagcgatat ggatcaatgg acacagaagc
     1621 catgcggaag ctcgcagacg cattgctttc cgttcaaaag aatcttgaag aaattaacgc
     1681 gaagaccgat caaatcaagt tggtgcctga cggcagtatc aaaatcggcg gggctgtctt
     1741 gggtgaacca gagaagctta aagagccttt tacaaatgca tgtaacgctt acaacagtca
     1801 acggtttgaa gaggcctatg tatgcgctac aaactatttg gcagtgtatg aggaatcgaa
     1861 acttcaagac gagggagccc tttttagaat gacccgcttt aacatccccg tcagtacagc
     1921 attaatgttt gctttcgcag cagaagggtc aatgtccaaa ggcaattaca aacttgcatt
     1981 gcagcaaatt gaatctgcga ttgcccttcg aaaagaaccc agagaaccct attttgatgc
     2041 aatcaaaaca gcgttgctga tgcaccttga tcaattcaag ctcgcaaacg agatgttgtt
     2101 tttctataaa tctcgacccg aagaggatcg gcatttctat tatagttcac ttttgatatg
     2161 gggctatttg tccgagttaa actttccaga aggctatgaa gctattaaaa aggaatttaa
     2221 tttaaacttt gacattagga ctccaatggc ctttcacacg agtgcgcaaa caaaagacgg
     2281 aaagcccatg gaggcatatg cactaatgct ttggaccggt ctagggactt ttaagcccat
     2341 aaactatgtg cccaaggaca agaaataaaa cccaacgagc aacggtctaa tgatcatgag
     2401 taacctcacc gaatccatcc tcgaagacgc ggcgctgacc tggttcaagg aactgggcta
     2461 cacggtcgcg ttcggcccgc acatcgcccc tgacgagcct gcggctgagc gcgactcgtt
     2521 cggcgatacg gtgctcacgg gacggttgcg cgatgccgtg gcgcgcctca atcctgatat
     2581 ccccgccgaa gcgcaggagg acgccatccg caaggttctg cgtgtggcca cgccctcgct
     2641 cgtccagacc aaccgcatct tccaccgcat gctcagaaac ggcgtggagg tcgagtaccc
     2701 gcgccctgac ggctccatcg ccggtgaccg tgtccggctc gttgatttcg agaccccaag
     2761 cgagaatgac tggctggccg tgaaccagtt cgcggttgtc gagggcccgt gcagccgccg
     2821 cgccgacatc actgttttcg tcaacggcat ccccctgggg ctaatcgaac tcaagaacgc
     2881 tgccgacgcc gacgccacga tctggagcgc gtaccagcaa gtgcagacct acaaggccga
     2941 gatcccgtcc ttgctcggct acaacgccgc gctcgtggtc agcgacggcg tcagcgcaag
     3001 gatcggctcg ctcaccgcat cccaggagtg gttcaaggtc tggcgcacaa tcgacggcga
     3061 gggcgacgcg cccaagaccg cgctggaact cgaagtgctc gtacgcgggg tgtttgaccg
     3121 caggcgtttc ctggacctga tccagcactt taccgttttc gaggaagacc cggacacggg
     3181 cgcggtcaac aagatcatcg ccggctacca ccagttccac gccgtcaacg ccgccgttga
     3241 agagacgctc agggcctcgg gcatgggcgt ctggggcgaa gcgcatgacc cgaaggacac
     3301 tgagggccgc tactggacag ggcgtatgaa cgacggcgcc gccggcgacc gcagggccgg
     3361 cgtggtctgg cacacgcagg gttccggcaa gagcctgtcg atgctctttt acgcgggccg
     3421 catcatccgg aaccgggaga tgcagaaccc gaccctgatc gtgctgactg accgcaacga
     3481 cctcgacgac cagctcttcg gccaattcca gcgctgccac gagatcctga accagacgcc
     3541 tgtccaagcg gagagccggg cgcacttaag cgagctgctc aatcgatcca gcggcggcgt
     3601 tatctttacg acgatccaga aattcctgcc ggagaagggc gagcgcatgc ccgcgctcag
     3661 cggccggcgt aacatcgtcg tgattgcgga cgaagcccat cgcagtcaat acgacctcat
     3721 tgacggcttg gcccgccata tgagggacac attgcccttc gcgactttta tcggcttcac
     3781 cggcacgcct atcgagaagg ccgacgccaa cacccgcgcc gtgttcggcg actacatcag
     3841 cgtctacgac atccagcgtg ccgtggctga caaggccacg gtgcccatct actacgagag
     3901 ccgtatcgcc aagctcgccc tcaacgccgc cgccgtgccg aaactagacg aagcgtttga
     3961 ggagatcacc gagggcgagg agctgacgcg caaggagaag ctcaagacca agtgggcggc
     4021 gctggaaacg ctcgtgggcg atcccaagcg catcgccctc gtggcgcagg acattgtcac
     4081 ccacttcgag cggcgctggg aggccatgga cggcaaggcc atggtggtct gcatgagccg
     4141 caggatctgc gtagagttgt acaacgccat catccgcctg cgtccggact gggccggcgc
     4201 cgaagacgat gacgtcgatg ccgaaagggg caaagactgc gtggtcaagg ttgtcatgac
     4261 cggatcagcc gacgacggtc ccgagtggca gccgcatatc cgcaacacgc tcaagcggcg
     4321 caagcttgcg gtgcggttca aggacagcaa ggatagtttc cgcatcgtga tcgtcaggga
     4381 tatgtggctg accggctttg acgcgccctg cctgcacacc atgtatctgg acaagcccat
     4441 gcagggccac agcctcatgc aggccatcgc ccgcgtcaac cgcgtgttca gggacaagcc
     4501 cggcggactc gtggtcgatt atctggggct ggccgaccag ttgagaaagg ccttggccgt
     4561 ctacaccgag agcggcggta tgggcgaccc cacctacgac accgcccagg ccattgccgt
     4621 gatgctggaa aaacacgagg tcgcgtgcag tatgctgcat gggtttaagt gggcgaagtg
     4681 gaccacgggc acggccacgg aaaggttgca gctcatcccg gcgggccaag agcacattct
     4741 ggcgcaggct gacggcaagc agcgcttcgt caaggccgtg acggagctgt cacgcgcctt
     4801 cgccctgtgc gcagcatccg acgaggcccg cgcgatccgc gacgacgttt ccttcttcca
     4861 gaccctgcag gcggcgctga acaagcagtc cttaggcagc cgcaagacac cggagcaact
     4921 cgacgccgcc gtgcgacagc tcgtgtccaa agccatcgtc accgagggtc aggtgatcga
     4981 cgtcttcacc gccgctggca tcgccaagcc cgacatctcg atcctgtccg accagtttct
     5041 ggccgaagtc cgggggctca agcacaagaa cgtggccgcc gaactgctgg agaagctgtt
     5101 gcgtgacgaa ctccgaatca ggtccagacg caacatcgtg caggccgagc ttttcagcga
     5161 gcggctcaag aaggctttga acgcctacca caatcgggcg atttccacgc tggagttcat
     5221 cgacgagctg atccgcatgg ccaaggatct ggatgccgcg tcgaagcgcg gacaggacct
     5281 ggggctcaac gacgacgagg tggccttcta cgacgcgctt gccaccaacg atgccgccgt
     5341 caaagtcatg ggcgacgaca agctcaaggt catcgccgtg aaactcctgt caaccgtacg
     5401 caagagtgtg accatcgact ggaacctcag agagagcgca cgggccaaga tccgggtcat
     5461 ggtcaggcgc atcctcagag aatacggcta cccgccagac cttcaggagg aggccgtcaa
     5521 gaccgtcctg gcccaagccg aactcgtatg cgcggactgg gagtcttaaa tggtgcactt
     5581 tgatgctttc acatgcttgt ttttaggaga cactggacat ggcatggcga cccggaggtg
     5641 aagctggaaa actgggcaac cgctatgagg ccaaatgggt tgttcggcag tgtgtgcgcc
     5701 tgctacgtga agaaatcgcg tcatcatgaa gtttgttgac cgcctgactg ctcatttgca
     5761 gacggcaaca gagccgacag aaggctggcg ttttcacgac cttcccggag tcttacttcg
     5821 tctttacgaa gagtcacaag ctgcacgaga ttccgagtgc gttggtgcct gtcttgatgc
     5881 gtgggacagg ttgtttgagg ccggttttga gcgcgtcaga gatttaagta atgagctaag
     5941 cagttatctt cctcaatcct aactgaaaac tccgcgcaca gcgcggccgc gccttcacgt
     6001 gataaaaaca agcgacttgc tcacgacgca taggcgtgat accctttcaa tcatgaaaaa
     6061 tgagattatt tttaatgtaa ctcaggatgc cgacggtgga tacacagccg aagcactggg
     6121 cgaggatatc ttcacgcagg cggacacatg ggaggaactc cgcgccaatg tccgcgaggc
     6181 cgtggccgcg tacacattcg accggcttga aaagccttcc gttcgcatcc gtctgacgcg
     6241 cgaagaggtt ttggcatgaa gttgccccgc gatctgtctg ggatccgctt agcccgccac
     6301 ctttgccgtt tgtggggata tacgcttgtc catcaggtcg gcagccatat cgttctccaa
     6361 accgagatgc caagtcacca ccggatcgtg att
//
LOCUS       DAOSRB010000160         6341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11308_length_6341_cov_6.753579, whole genome shotgun sequence.
ACCESSION   DAOSRB010000160 DAOSRB010000000
VERSION     DAOSRB010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11308_length_6341_cov_6.753579"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1191
                     /gene="pheS"
                     /locus_tag="PLZ74_08490"
     CDS             154..1191
                     /gene="pheS"
                     /locus_tag="PLZ74_08490"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOR98400.1"
                     /translation="MTLAELDSQQADSLAEVASAADAAALEKVRVRYLGRNGLLPAII
                     RQMKEVPAAERPEFGRRVQAWRSALEEALAGRMADAVAAREEQAAFDCTLPGRWPACG
                     AKHPVSRVIEETAAIFARLGFTVADGPDVETRFNNFTALNTPAHHPSMDASDTFWLTD
                     DRLLRTQTSPVQIRMMTCHTPPVRIITPGRTYRRDTTDATHSANFHQIEGLYVDTKPV
                     SLADLKSTLSYYAREMMGAKAGVRFRPHFFPFTEPSVEVDFSCHVCGGKGCRICKMSG
                     WIEIAGAGMVDPRVYEHVNRARGDMAYDPATVYGYAFGFGVERIAMVKYGIPDIRWLY
                     ENDTRFLSQLA"
     gene            complement(1311..1979)
                     /locus_tag="PLZ74_08495"
     CDS             complement(1311..1979)
                     /locus_tag="PLZ74_08495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOR98401.1"
                     /translation="MTHTHRRLTLEFAALYLVLPVFLFLIRHQGIPLPVLPALWCAAG
                     PAAYCLVRRHNWDLRAFFALPASARRIGTLALRAALAGAVLTGTLWLTAPDSLFELPR
                     RNPRLWAIVMTAYPVLSVFPQGILYRALFFTRYAVLFQNRRAAWLASAVVFSLAHLLF
                     NNFWALAWTLLGGLLINRSYLRHGSLMAAAAEHAVYGQCLFTCGWGRFLYHGTTGLAE
                     SLIP"
     gene            complement(2044..2232)
                     /locus_tag="PLZ74_08500"
     CDS             complement(2044..2232)
                     /locus_tag="PLZ74_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98402.1"
                     /translation="MLLPTLPACLPELPISAYAGEIAAALRTHPVVIVSGDTASGKTT
                     QVPVSAKRLAALWKRVFA"
     gene            complement(2325..3095)
                     /locus_tag="PLZ74_08505"
     CDS             complement(2325..3095)
                     /locus_tag="PLZ74_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOR98403.1"
                     /translation="MKTLIAVLLCSGCLAYARAAQTACEMTVAEVFTDADGGTLPYRI
                     YLPAGIPAERKLPLVLFFHGAGERGTNNVSQLVHGVMPLIRYGQNAKDPAILLVPQCP
                     NGMQWVSVPWGDSRHTMPAQPSIPMRRALALLREKIRTLPVDPARVYVTGISMGGYGT
                     WDAIQREPDLFAAAIPVCGGGDTACAPRIRHIPIWTFHGEKDGAVPVCRSRDMAEALK
                     SCGGNVRYREYPGAGHGVWTPTYNDTEVLTWFFSQRKP"
     gene            3458..4978
                     /locus_tag="PLZ74_08510"
     CDS             3458..4978
                     /locus_tag="PLZ74_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOR98404.1"
                     /translation="MADIIGFDNDTYLTEQTEEIKRRAEKSGNKLYLEFGGKLMHDMH
                     AARVLPGYDPNVKLRLLQKLKDKAEIVLCVFAGDIERKKMRADFGISYDTDALKMIDG
                     LRSWGLLIRAVVITRFCGQHAAKQFRARLEHRGIKVYYHYVTQGYPANVDTIVSPEGY
                     GANEYIETERPIVVVTGPGPGSGKLATCLTQLYHDRNAGRLAGYAKFETFPVWNLPLE
                     HPINIAYEAATVDLNDANMVDPYHLQAYQKVAINYNRDIDAFPLLRAIWEKITREPCP
                     YKSPTDMGVNRVGFGITDEAVVCAAAKQEVIRRYFRHLCEFSSGQTEHITVDRIELLM
                     QKLDLVPEDRVVVTPARQAGQDAMKKGKGRNGIFCGAALQLKDGRIVTGKNSEQMHAA
                     ASMVLNAIKELSGIPDQIHLIAPQVVQSIVHMKHDILKGKYTSLNLDEALIGLAVSCA
                     TNPAAQIAMERLIDLRDCEVHLTHMVTPGDEAGLRRLGMRCTSDPFFATSELFIDA"
     gene            complement(4965..5459)
                     /locus_tag="PLZ74_08515"
     CDS             complement(4965..5459)
                     /locus_tag="PLZ74_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOR98405.1"
                     /translation="MKQDGGMDWEKWVPKDRAVLLFIRYEGRLLLIVKKRGLGRGKVN
                     APGGRLEPGETPEQAAVRETREEVGLTPNGLRPAGRLAFAFMDGYSLSCCVFTASGYE
                     GTLTETDEAMPFWCAESDIPYDRMWADDRLWIPLMLAGKAFEGRFVFDGDRMLWHRLT
                     LTRQ"
     gene            complement(5558..5980)
                     /locus_tag="PLZ74_08520"
     CDS             complement(5558..5980)
                     /locus_tag="PLZ74_08520"
                     /inference="COORDINATES: protein motif:HMM:NF027223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase
                     domain-containing protein"
                     /protein_id="HOR98406.1"
                     /translation="MNTKKKIYLSGPIMDEHPDLATTWRERAKEALRDHFILLDPMRR
                     NFKDREVDSANEIVEFDLQDVRDADILLVNYNKSSIGTSMEVFFAAHNLGKFVVAFSP
                     FCFQDCSPWMVRYCTKILPSLDTAIDYIRDNFISRHVE"
     gene            complement(5977..>6341)
                     /locus_tag="PLZ74_08525"
     CDS             complement(5977..>6341)
                     /locus_tag="PLZ74_08525"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368298.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOR98407.1"
                     /translation="ALAATLTARGFHLVSGGTDNHLMLMNVARNGLTGKDAAAALDAA
                     GIICNKNTIPFDKNSPFVTSGIRIGTASVTTRGMREPDMQKIGDWIADILSDLSNTAL
                     IEKVRTEVAALTATFHVP"
BASE COUNT         1318 a         1894 c         1940 g         1189 t
ORIGIN      
        1 aatgcatatt cggagcaact gaatgcgacc gtgcgtcata aagacccagt tttggccgca
       61 tttactttgc caagtgacct gtaaaacgcg gttgcttttc ggggaggagg cgcggtaaac
      121 taaaaagcat tctcaaaaaa aggttacggg tatatgactc ttgcggaatt ggattctcaa
      181 caggcggact ctttggctga agtggcctct gcggcggatg ccgccgcgct ggagaaggtg
      241 cgtgtccggt atttggggcg caacggattg ctgcccgcga tcatccggca gatgaaggag
      301 gttccggctg ctgagcggcc ggagttcggc aggcgggtgc aggcgtggcg ctccgcgctg
      361 gaggaggcgt tggccggacg catggcggat gccgtcgcgg cacgggaaga acaggcggcc
      421 ttcgactgca cgttgccggg acgatggcct gcctgcgggg cgaaacatcc ggtctcacgc
      481 gtgatcgagg agaccgccgc gatctttgcg cggctgggct tcacggtcgc ggacgggccg
      541 gatgtcgaga cgcgcttcaa caactttacg gcgctcaaca cgcccgcgca ccatccctcg
      601 atggacgcgt ccgacacctt ctggctgacg gatgaccggc tgctgcgcac gcagacctcg
      661 ccggtacaga tccgcatgat gacctgtcac acgccgccgg tgcgcatcat cacgccgggg
      721 cgcacctacc gccgtgacac gaccgacgcc acgcacagcg cgaatttcca tcagatcgag
      781 ggcctctacg ttgatacgaa gccggtctcg cttgcggatc tcaagtctac gttgtcctat
      841 tacgcgcggg agatgatggg cgccaaggcg ggcgtgcgtt tccgtccgca cttcttcccc
      901 ttcaccgagc cgagcgtcga agtggacttc tcgtgccatg tgtgcggcgg caagggatgc
      961 cgcatctgca agatgagcgg ttggatcgaa atcgctggcg cgggcatggt ggatccgcgc
     1021 gtatacgaac acgtcaatcg ggcgcggggc gatatggcgt acgaccctgc gacggtctac
     1081 ggctatgcct tcgggttcgg tgtcgaacgc atcgcgatgg tcaagtacgg cattcccgac
     1141 atccgctggc tctacgagaa cgacacccgt tttctgagcc agttggcgtg atgcggatct
     1201 aatgcggggg gagcccgcaa cccaagggag acgggcgacg tcgaaaacgc cgaagcaacg
     1261 gtagggacgc atccccgagg cgtccgtcca gggcagggac cggcggcggt ttatggaatc
     1321 aggctttccg cgaggccggt ggtgccgtgg tacaggaaac gaccccagcc gcaggtgaac
     1381 aggcactgtc cgtagacggc atgttccgca gccgcagcca tcagcgagcc gtgccgcaga
     1441 tacgagcggt tgatcaacag ccctcccaat aaagtccagg caagtgccca aaaattgttg
     1501 aagagcaggt gggccagact gaacacgacc gcactcgcca gccaggccgc gcggcggttt
     1561 tggaacagca ccgcataccg tgtgaaaaac agggcgcggt agaggatgcc ttgcgggaat
     1621 acggaaagca cgggatatgc cgtcatcacg attgcccaga gccggggatt gcgtcgcggc
     1681 aactcgaaca gcgagtcggg tgcggtcagc cacagcgtcc cggtgaggac ggcgccggcc
     1741 aaggcggcgc gcaacgcgag tgtgccgatc cggcgcgcac tggccggaag ggcgaaaaag
     1801 gcccgaaggt cccagttgtg ccgacggacc agacagtacg ccgccggacc cgccgcgcac
     1861 caaagagccg gcagtaccgg caggggaatc ccctgatggc ggatgagaaa caagaaaacc
     1921 ggcagcacga gatagagggc cgcgaattcc aacgttaaac gacggtgtgt gtgtgtcatg
     1981 gcgagccttt ccggaaccgg ggcatgatgc cggaggggaa ccggggtgtc aagatccggg
     2041 cggtcacgcg aaaacccgct tccacagggc cgcgagacgt ttggcggaca cgggaacctg
     2101 cgtggtcttg ccggaagcgg tatcgccgct gacgataacc accggatggg tgcgtagagc
     2161 cgcggcgatc tcgccggcat acgcggagat gggcagttcc ggaagacagg ctggcaaagt
     2221 cggaagcaac atgggaaata cggtgttccg aacggttcgg ggttgccgcc gcacctgccg
     2281 gatgagtttg ggccaggccc ggtgtccggc cgattgcggg aaggctacgg tttgcgttgc
     2341 gagaagaacc aggtaagcac ttcggtatcg ttatacgtcg gtgtccagac cccgtgcccc
     2401 gcgcccggat attcacggta ccgcacgttg ccgccgcacg acttgagcgc ttccgccata
     2461 tcacgcgacc ggcagaccgg caccgcgccg tccttctcac cgtggaacgt ccagatcggg
     2521 atgtgtcgga tacgtggcgc gcaggcagtg tcgccaccgc cgcagaccgg tatggccgca
     2581 gcgaacaggt ccggctcacg ctggatggcg tcccaggtgc cgtatccgcc catagagata
     2641 ccggtgacat aaacgcgcgc gggatcgacg ggcagggtac gtatcttttc gcggagcagc
     2701 gccagcgcgc gccgcatagg gattgacggc tgcgccggca tggtgtggcg ggaatctccc
     2761 cacgggacac ttacccactg cataccgttc gggcactgcg gcaccagaag gatcgcgggg
     2821 tctttggcgt tctgcccgta tcggatcagc ggcatcacgc cgtgcaccag ttggctgaca
     2881 ttgttggtgc cccgctcgcc cgcaccgtgg aaaaacagca ccagcggaag tttgcgctcc
     2941 gccgggattc cggcaggcag gtaaatccgg taggggaggg tgccgccatc cgcatcggtg
     3001 aacacttcgg caacagtcat ttcgcaggca gtctgggctg cccgcgcata tgcgagacac
     3061 cccgaacaca acagaacagc tattagagtt ttcatcattt ctccggaggg aaagggccgg
     3121 tttgcggtat gcatcccgtg ccggatccga atgaggcgta gcatagcaac gcgccgtcgg
     3181 atctgtcaaa tgcgcggaga ggatgacgat tcgcgtgaaa aatcagggca gcacttttac
     3241 attcgccgtt gcgagccgct gcgtggtcgc aatacgcggt ccgtcaaaat tatagaaaag
     3301 acaccgtagg ttttactatg acatgccgcg acggattcgc gtccgcagaa aatgagttta
     3361 cgtcgccacc ggttttgcgc tatagtcctg tcccggccac gcattaacgg ttgaccgttt
     3421 ttgtttctct tccttatcgc gcggaggcag gttgtttatg gcggacataa tcgggtttga
     3481 caatgacacg tacctcactg aacaaaccga ggaaatcaaa cgccgcgctg aaaagagcgg
     3541 caacaagctt tatctcgaat tcggcggcaa gttgatgcac gatatgcacg cggcccgtgt
     3601 cctgcccggg tatgatccga atgtcaaact acgccttctg caaaagctca aggacaaggc
     3661 cgagatcgtg ctctgtgtct ttgcgggcga catcgaacgc aaaaaaatgc gcgcggattt
     3721 cggcatctct tacgacaccg acgctcttaa gatgatcgac ggtctacgca gttggggcct
     3781 gctgatccgt gccgtggtca tcacccgttt ttgtggacag catgccgcca agcagttccg
     3841 cgcccgcctc gagcaccgcg gcatcaaggt ctattatcac tacgtgaccc aaggttaccc
     3901 tgccaacgtc gacacgatcg tcagccccga gggttacggg gcgaatgagt atatcgagac
     3961 tgaacggccg atcgtggtcg tgaccggacc gggccccggc agcggcaaac tggccacctg
     4021 ccttactcag ctctaccacg accgcaatgc ggggcgcttg gccggttatg ccaaatttga
     4081 aacgttcccg gtctggaatc tgccgctgga gcaccccatc aacatcgcct atgaggccgc
     4141 cacggtcgat ctcaatgatg ccaacatggt ggatccctat catcttcagg cctaccaaaa
     4201 ggtggcgatc aactacaacc gcgacatcga tgctttcccg ctgctgcgtg ccatctggga
     4261 gaaaatcacc cgcgaaccct gtccctacaa atcaccgacc gacatgggcg tcaaccgcgt
     4321 gggcttcggc atcaccgacg aggcggtcgt ctgcgcggca gccaaacagg aggtgatccg
     4381 ccgctacttc cgccatctct gcgagttctc ctccggacag accgaacaca tcactgtgga
     4441 ccgcattgaa ctgttgatgc aaaaactgga tctggtcccg gaggaccgcg tggtcgtgac
     4501 gcccgcgcgc caagcgggac aggacgccat gaaaaaaggc aagggacgca acgggatatt
     4561 ctgcggcgca gccctgcaac tcaaggacgg acggatcgtc acgggcaaga actccgagca
     4621 gatgcacgcg gcggcaagca tggttctgaa cgcgatcaag gaactctcag gcattccgga
     4681 tcagattcat ctgatcgccc cgcaggtcgt gcagtcgatc gtgcacatga aacacgacat
     4741 cctcaaaggc aaatacacca gcctgaatct cgacgaggcg ctgatcgggc tcgcggtcag
     4801 ttgcgcgacc aatcccgccg cccagatcgc gatggagcgg ctgatcgacc tgcgcgactg
     4861 cgaggtccac ctgactcaca tggtgacacc cggcgatgag gcgggcttgc ggcgtctggg
     4921 catgcgctgc acgagcgacc ctttctttgc cacgtccgag ctgtttattg acgcgtgagc
     4981 gtcaaccggt gccagagcat ccggtcgcca tcgaaaacga aacgcccctc gaacgctttg
     5041 cctgcgagca tcagcggaat ccaaagccgg tcatcggccc acatgcgatc gtacgggatg
     5101 tcggattccg cgcaccagaa gggcatagcc tcgtcggttt ccgttagcgt gccctcgtag
     5161 ccgctggcgg taaagacgca acagctcagc gaatagccgt ccataaaggc gaaagcaagg
     5221 cgaccggccg ggcgcaggcc attgggcgtc agccccacct cttcgcgcgt ctcgcgcacc
     5281 gccgcctgct cgggcgtctc acccggttcg aggcggccac ccggcgcgtt cactttgcct
     5341 cggcccaacc ctcttttctt gacgatcagc agaagccgtc cttcataccg gataaataag
     5401 agcaccgcac ggtccttcgg cacccacttc tcccaatcca tcccgccatc ttgtttcact
     5461 tcttgcttct tacctattac ctctcaccta ttacttctta cctctcacct attacctatt
     5521 acctattact tcttacctat tacttcttac ctattaccta ttccacatgc ctcgagatga
     5581 aattgtcacg gatatagtcg atcgccgtat ccagcgatgg caggatcttg gtgcagtaac
     5641 gcaccatcca gggcgagcag tcttgaaagc agaaaggcga aaaggccacc acgaacttac
     5701 ccagattgtg tgccgcgaaa aagacctcca tcgaggtgcc gatcgaggat ttgttgtagt
     5761 tcaccagcag gatatcggca tcgcggacat cttgaaggtc gaattcgacg atctcattgg
     5821 cactgtcgac ttcgcggtcc ttgaagttcc gccgcatcgg gtcgagcagg ataaaatggt
     5881 cgcgcagggc ttccttggcc cgttcgcgcc acgttgtagc cagatcggga tgctcgtcca
     5941 tgatcgggcc ggagagatag atctttttct tcgtgttcat ggcacgtgga aagtggcggt
     6001 cagagcggca acttccgtgc gcaccttttc gatcaatgcc gtatttgaga ggtcggagag
     6061 gatgtcagcg atccagtcgc cgatcttttg catgtcaggc tcccgcatgc cgcgcgtcgt
     6121 gaccgacgca gtgccgatac ggatgccgga ggttacgaac gggctgttct tgtcgaacgg
     6181 aatggtgttc ttgttgcaga taatgccagc tgcgtccagc gcggcggcgg cgtccttgcc
     6241 ggtgaggccg ttgcgcgcga cattcatcag catcaaatgg ttgtccgtac cgccggagac
     6301 cagatggaag ccgcgcgccg tgagagtcgc ggccagcgcc t
//
LOCUS       DAOSRB010000161         6333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11321_length_6333_cov_7.576457, whole genome shotgun sequence.
ACCESSION   DAOSRB010000161 DAOSRB010000000
VERSION     DAOSRB010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11321_length_6333_cov_7.576457"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..4177)
                     /locus_tag="PLZ74_08530"
     CDS             complement(32..4177)
                     /locus_tag="PLZ74_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOR98408.1"
                     /translation="MRVHHPVLLAMVTVCAAVLRTGAFETTNWLSSVAEGYYNDPANW
                     TGGFLPTNGNTGQFTGNQDYVVRFPQGGLVENSVTKVSGLSSGRSVTFDTRGTWWLKA
                     GCDAWPNDWTAFQLGANSSHFFNIEGVLKAAANDYPVMEMSNALFRVQYHSTGMTNVL
                     EEGLLNLYNPGGIHRSAHSLITGSTAPANSLILFKTNSMLRANNVRLRGNRNGHLMRF
                     EGGTHEIYNGMTIAEGGANAGTTCTVEVAGGTLAVRSGSVYVDNKVGTHGNLVIEGSG
                     DMTVRDLVYVAYNATNCSGTVILRDAGRLAVGGDFRIAQFNKTTGELRIADEAALSVA
                     SAMRVSYASASTAAVDVAGSAALAVTGQVTVACASESYAEMAVRDNATVYAGGDLLVG
                     DAGGNAVFTLDDAATLVVKGAALTVAGNSGTASRGRLILTGGTLEANAVRGGVGALCR
                     GGTGGAELLADGTTLRAGGATTGLFLGGFDQAELGAQGLTLDSAGTDLGMGQPFSDLP
                     GATGLFVKTGAGTLSVSNSLHGRTEIARGVLRASEPAAFFGREIAVSNIASLSLEGEA
                     VTLTAGDLTLGSPGGRAILALDAGDSVSVTNALTLHGCAIYFGGAETAGTHTLIRSAG
                     TIDPSCLDHVELLNPAAGKAYAFAIGTDGGGETIQLIVSAYTIADAEWDGSESADWNT
                     ADNWTPAGVPTYGTRAKFGAAAARKAVTLSAPGLTTFMEFDSDAGYTLGGTQPLTLPA
                     GMIDNKLGEHEIALPLTLSGEVALPTAAAATTTVSGPVSANLAARVAKSGSGMVVLSG
                     DNAGFQGAWRTGGGRMVFATAGAWGAANSAADAVTVGAGTLTYSGTPAEVSKGIMVDA
                     GGSTLSAILEVKSDLTVNGTVTNHSGILIKRGVGTLTLNVGAGHGNLSSGSGGSASNN
                     DPSGIITFPENGDSPATDNGLGGFNLLEGTLRIRGVNSTLSIVNQRHFGVLGGAVSSC
                     LADPTLELDAVRLNQGGSGQHLIVGNQIQAGSLARSPTLRLVNGAHLAMDHLRIGRNS
                     AAMVTPTLIMSNATVEASWQVSFGADSTVTPYVRIMEGSQIIAKGGAPWGGGICFSRN
                     VDMVVSGNSVVSQTHSDGDMRFNEGGADGTVRWEDGAVMRFAKFSGRNHNTADGLDMI
                     FDNATMEILANGYSYSTRADKQSFIIEDGGLTVKVGSGIRHELTFPLTGAGALTKTGA
                     GELVFAAGLNYTPSATNLTGLATGDYTGGTLVQEGALSVSNGTIRTDAAVRVAAGARL
                     NLSNSGVALGEVSGSGLITNGTLTAGYRCRVTPAGADRLTLSDVTLPSGFMVTFDAEA
                     GSELVSGQKVAIADLAGATVPNLDKWSARHVGEKLSARFTLEGGTVYANVFYSAGTLF
                     QLR"
     gene            4398..5420
                     /gene="galE"
                     /locus_tag="PLZ74_08535"
     CDS             4398..5420
                     /gene="galE"
                     /locus_tag="PLZ74_08535"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOR98409.1"
                     /translation="MRILVTGGAGFIGSHTSVELLQAGHEIVIVDNLCNSSAESVRRV
                     AEITGARPIFRKVDLLDREALDTVFAEDGPFDAVIHFAALKAVGESVGIPLTYYRNNL
                     SGTITLCEVMTAHGCRNMVFSSSATVYGDPASVPIREEFPTPGATNPYGWTKLMMEQI
                     FRDLHRADPAWNIILLRYFNPIGAHASGRIGEDPAGLPNNLLPYVAQVAIGRLPSLSV
                     FGNDYPTPDGTGVRDYIHVVDLAVGHLKALEFLHTGPGLEVFNLGTGKGSSVLEVVRA
                     FEQASGRAVPYRIMPRRPGDVAACWADPAKAERVLGWKAERDLRVMCEDAWRWQSANP
                     NGYAGG"
     gene            5423..6253
                     /locus_tag="PLZ74_08540"
     CDS             5423..6253
                     /locus_tag="PLZ74_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOR98410.1"
                     /translation="MSEWTDILDGLTDQITLLKELGLRTVELDPAVIRALGRRAAPAA
                     DATRPPPDSQPPTVPAPIAPEPFTAPRVAPIEETQALSPEARRAALDELAARIASCTA
                     CPLHQSRNRTVPGQGNFNSPDILFIGEAPGAEEDQQGVPFVGAAGQLLTRMIAAMGYT
                     RDQVFIANICKCRPPRNRPPTPDEAAICLIYLRQQIRLIRPKTIILLGGTAIKALLNT
                     QVGVNRIQGEWTQYEGIPVMPTFHPSYLLRFEPAKRDAWQALKRVLAHLGKPVPSSSR
                     "
BASE COUNT         1121 a         2211 c         1870 g         1131 t
ORIGIN      
        1 agcattcgtt ttcattggaa aacaaagcta tctatcggag ctggaacaag gttcccgcgc
       61 tgtaaaagac gttggcgtaa accgtgccgc cctccagcgt gaaccgggcc gagagcttct
      121 cgccgacatg gcgcgcgctc cacttgtcga gattcggcac cgtggcgccg gcgagatccg
      181 caatcgccac cttctgcccg ctgacgagtt ccgaccccgc ctccgcgtca aaggtcacca
      241 tgaatcccga cggcagcgtc acgtccgaca gcgtcaggcg gtcggcgccc gcgggcgtca
      301 cgcggcagcg gtaccccgct gtcagcgtcc cgttcgtgat caggcccgac cccgagacct
      361 caccgagggc gaccccgctg ttgctgaggt tcagccgcgc acccgccgca acccgcaccg
      421 ccgcgtccgt gcggatcgtg ccgttgctca ccgagagcgc gccctcctgc accagggttc
      481 cgcccgtgta gtcgccggtc gcaagaccgg tgaggttggt cgcgctcggc gtgtagttca
      541 gccccgccgc gaagaccagc tcgcccgcgc ccgttttggt aagcgcgccc gcgccggtaa
      601 gcgggaaggt caactcatgc cggatgccgc tgccgacctt caccgtcaat cccccatcct
      661 caatgatgaa gctctgcttg tcggcgcgcg tggaatacga gtagccattc gctagaatct
      721 ccatcgttgc gttgtcaaaa atcatatcca atccgtccgc cgtgttgtgg ttgcgcccac
      781 tgaatttcgc aaagcgcatc accgcaccgt cctcccagcg gacggttccg tcggctccac
      841 cttcgttgaa ccgcatgtca ccatcggaat gggtttgcga caccacgctg ttcccgctga
      901 ccaccatatc gacgtttcga gagaagcaga tgccgccgcc ccatggagcc ccgcctttcg
      961 cgattatctg gctaccctcc atgatgcgca catacggagt cacagtactg tcagcgccga
     1021 aggacacctg ccaggaggct tcgaccgtcg cattgctcat aatcagcgtc ggcgtgacca
     1081 tggccgccga attccgccct atccgaaggt ggtccatcgc gagatgagcg ccgttcacca
     1141 gccgcagggt cggtgaacgc gccagactgc ccgcctgaat ctggttgccg acgatgaggt
     1201 gctgcccgct gccgccctga ttgagacgca cggcatcgag ttcaagcgtc ggatcggcca
     1261 gacaggagct gaccgcaccg ccaagaactc cgaagtgccg ctgattcacg atcgaaagcg
     1321 tgctgttgac gccccggatc cgtagcgtac cctccaacag gttgaacccg cccaacccgt
     1381 tgtctgtggc cggagagtcc ccgttctccg ggaacgtgat gatgcccgaa ggatcattat
     1441 tactggccga accgcctgat cccgacgaaa ggttcccgtg cccggccccg acattcaggg
     1501 tcagcgtgcc gaccccgcgc ttgatcagga tgccggaatg gttggtgacg gttccgttga
     1561 ccgtcagatc ggacttcact tccagaatcg ccgagagggt gctgccgccc gcatccacca
     1621 tgatcccctt gctgacttcc gccggcgttc cgctataggt caaggttccc gcgccgaccg
     1681 tgaccgcgtc cgcagcacta ttggccgcgc cccaggcgcc tgccgtggca aagaccatcc
     1741 gtccgccgcc ggtccgccac gcgccctgga acccggcatt gtcgcccgac aaaaccacca
     1801 tgccggaacc gctcttggcg acgcgcgccg ccagattcgc cgagaccgga ccgctcaccg
     1861 tggtggtggc cgccgccgcg gtcggcagcg cgacctcgcc cgagagagtc agcggcaagg
     1921 cgatctcatg ttcgcccagc ttgttgtcga tcatgccggc cggcagtgtg agcggctgcg
     1981 tgccgccaag cgtgtagccc gcatccgaat cgaactccat aaaggtcgtc agccccggcg
     2041 cgctgagcgt gaccgccttc cgcgccgcag ccgcgccgaa cttggcgcgc gtgccgtagg
     2101 tcggcacccc ggccggggtc cagttgtccg ccgtgttcca gtccgcgctc tccgagccgt
     2161 cccactccgc gtccgcaatc gtatacgcgc tgacgatcag ctggatggtc tccccgccgc
     2221 cgtccgtacc gatggcaaag gcatatgcct tgcccgccgc cggattgagg agttccacat
     2281 ggtccaggca cgacggatcg atggttcccg cgctacggat cagcgtgtgc gtgcccgccg
     2341 tctccgcacc cccgaaatag atcgcacagc cgtgcagcgt caacgcattg gtcacgctca
     2401 cactgtctcc ggcatccaga gccaggatcg cccggccccc gggcgagccg agcgtgagat
     2461 ccccggccgt cagcgtcacg gcctcacctt ccagcgaaag cgaggcgatg ttcgaaaccg
     2521 cgatctcacg gccgaaaaac gccgcgggtt cgctggcgcg cagaacgccc cgcgcgatct
     2581 cggtccgtcc gtggaggctg ttcgagacgg agagggttcc cgcgcccgtt ttcacgaaca
     2641 gccccgttgc cccgggaagg tccgagaacg gctgccccat cccgaggtcc gtaccggccg
     2701 aatccagagt gagtccctgc gctcccagtt ccgcctgatc gaaccctccg aggaacagtc
     2761 cggtcgtggc gccgcccgcc cgcagggtcg tcccgtccgc cagcagttcg gctccgcccg
     2821 ttccgccccg gcacagcgcg ccgaccccgc cgcgcaccgc gttggcctcc agcgtgccgc
     2881 ccgtcaggat cagccgtccc cggcttgcgg tcccgctgtt cccggccacc gtaagggccg
     2941 cgcctttcac gacaagcgtt gcggcatcgt ccagcgtgaa cacggcgttg ccgccggcat
     3001 cgccgaccag aagatccccg cccgcgtaga ccgtggcgtt gtcccgcacc gccatttcgg
     3061 catacgactc gctggcgcag gccaccgtga cctggccggt caccgccagc gcggcgctcc
     3121 cggcgacatc cacggcggcg gtcgaggccg aggcgtaaga caccctcatg gccgaggcga
     3181 cactcagcgc ggcctcgtcg gcgatgcgca gctcgccggt cgttttgtta aactgcgcaa
     3241 ttcggaaatc cccgcccacg gcaagccgcc cggcatcccg caagatcacc gtgccgctgc
     3301 agttggtcgc gttatacgcc acatagacca ggtcccgcac cgtcatgtcg ccggaaccct
     3361 cgatgacgag attcccgtgg gtcccgactt tattatcgac atacaccgag ccgctgcgca
     3421 ccgcaagggt gccgcccgcg acctctaccg tacacgtcgt tcccgcattc gcgccgccct
     3481 cggcgatggt catgccgttg tagatctcgt gcgtgccgcc ctcgaaccgc atgagatggc
     3541 cgttccggtt cccgcgcaaa cggacgttgt tggcccgcag cattgagttc gtcttgaaga
     3601 ggatcaggct gttggccggt gcggtggaac cggtaatcag cgagtgcgca ctacggtgga
     3661 tcccgccggg gttgtacaga ttcagcagcc cctcctccag cacgttcgtc atgccggtgc
     3721 tgtgatactg cacccggaag agcgcgttcg acatctccat gaccgggtaa tcgttggccg
     3781 ctgctttcaa caccccctcg atattgaaaa aatgcgagga gttcgcgccc agctgaaaag
     3841 cggtccaatc gttcggccag gcgtcgcaac cggccttgag ccaccaggtg ccgcgtgtgt
     3901 cgaaggtgac cgagcggccg ctcgaaaggc cactgacctt ggtcacgctg ttctcgacca
     3961 gtccgccctg gggaaaccgg accacatagt cctgattgcc ggtgaactgc ccggtgttgc
     4021 cgttggtggg caggaagccg cccgtccagt tcgcggggtc attgtagtag ccttccgcga
     4081 cagaggagag ccagttggtc gtttcaaagg cacccgtccg caacaccgcc gcacacaccg
     4141 tgaccatcgc caacaaaaca ggatgatgca ccctcatccg ccacctcctc aactccttca
     4201 accattatcc attcggaccc gcattgtaat taaagatgtt cctaaacgat gcggtccatt
     4261 atccaacgac aaattcagtg tatcgcacgc ctccttcccg aatcaagacc gaaaacccga
     4321 tgcgcggaaa tcaacttcgg attggttatc cggacgattt cggttaaact ggcactgttc
     4381 tttcagaaag gaccctcatg agaattcttg ttaccggcgg tgccgggttc atcggtagcc
     4441 acacttcggt cgagttgctg caggccgggc acgaaatcgt gatcgtcgac aacctttgta
     4501 acagcagtgc ggaaagcgtg cggcgcgttg cggagatcac tggcgcgcgg ccgattttcc
     4561 gcaaggttga cctgctggac cgggaggcgc tcgacacggt ctttgcggaa gacggaccgt
     4621 ttgatgcggt gatccatttc gcggccctca aggcggtcgg cgaatctgtg ggaatccctc
     4681 tcacctacta ccgcaataat ctttcgggca cgatcacgct ttgcgaggtg atgaccgcac
     4741 acggttgccg taacatggtt ttcagctcat ccgcgacggt ctacggtgat cctgcctctg
     4801 tgccgatccg cgaagagttt cccacgcccg gcgccaccaa cccgtacggc tggaccaaac
     4861 tgatgatgga gcagattttc cgtgacctgc accgcgccga cccggcgtgg aacatcattc
     4921 tgttgcgtta tttcaatccg atcggcgcgc acgcgagcgg ccggattggc gaggatcccg
     4981 ccggcctccc caacaacctg ctgccttatg tggcccaggt ggccataggc cgccttcctt
     5041 ctctatcggt gtttgggaat gactacccga cgcccgatgg aacgggcgtg cgggactaca
     5101 ttcacgtggt ggatctggcg gtcggccacc tgaaggcact ggaatttctc cataccggac
     5161 cggggctgga ggtcttcaac ttggggaccg gcaagggaag cagtgtgctg gaggtggtgc
     5221 gggctttcga acaggcttcg ggccgtgccg taccctaccg gatcatgccg cgccgccccg
     5281 gtgacgtggc cgcctgttgg gccgatcccg ccaaggcaga gcgcgtgctg ggctggaaag
     5341 ccgagcgtga tctgcgtgtc atgtgcgaag acgcctggcg ctggcaatca gccaacccca
     5401 acggatacgc gggaggttaa tcgtgtcgga atggaccgac atcctggacg gtctcacgga
     5461 ccagatcacg ctcctcaaag aactggggct gcgtaccgtg gaacttgatc ccgccgtgat
     5521 tcgggctctg gggcggcgcg ccgccccggc ggccgacgcg acacgccccc caccggacag
     5581 ccagccgcca acggtcccgg cccccattgc gcccgagcct ttcacggcac cccgcgtcgc
     5641 tccgattgag gagacgcagg ccctctctcc tgaggcccgg cgcgccgcac tcgacgaact
     5701 ggcggcccgt atcgcctctt gcaccgcttg cccgctgcac cagagccgca accggacggt
     5761 tccgggtcag ggaaatttca acagccccga cattctgttc atcggcgagg cgcccggcgc
     5821 ggaggaggat cagcagggag tgccgttcgt cggcgcggcg ggtcagttgc tgacgcgcat
     5881 gatcgcggcg atgggctata cccgcgacca agtgttcatc gccaacatct gcaaatgccg
     5941 tccgccccgg aaccgtccac cgacaccgga cgaagccgcg atctgcctga tctatctgcg
     6001 gcaacagatc cgcctcatcc gcccgaaaac cattattctg ctgggcggca cagccatcaa
     6061 agcgctgctg aacacccaag tgggcgtcaa ccgcattcaa ggcgagtgga cccagtatga
     6121 gggcattccc gtgatgccga ccttccaccc ttcctacctg ctgcgcttcg agccggctaa
     6181 acgcgacgct tggcaagcgc tcaaacgagt gctcgcgcat ctcggcaagc cagtcccctc
     6241 ctcatcgcgt tgagaaaacc gcccggccac gcggggtagg gacgcccgct tctcccgtca
     6301 gaccttatgt cgacccgcga cccgcgacga gcg
//
LOCUS       DAOSRB010000162         6272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11478_length_6272_cov_7.171787, whole genome shotgun sequence.
ACCESSION   DAOSRB010000162 DAOSRB010000000
VERSION     DAOSRB010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11478_length_6272_cov_7.171787"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLZ74_08545"
     CDS             <1..574
                     /locus_tag="PLZ74_08545"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOR98411.1"
                     /translation="KRRAISRITREYEQKRAALGNPVSPTLFRNPKFYFAAIVVFVFL
                     GILLSNTTDKAVRRMEVTPHMRAIRNLDVLAEALGRYHFHTGSFPDAVPGLAALVRDP
                     AVPKWDGPYIRFLRNDPWNTPFVYAPPAGNGLPTLFSCGEDRIPHTPDDLFPDPARFD
                     PGTAWTNGWVSAEQRLPGVTVLPSSPDGKR"
     gene            605..1402
                     /locus_tag="PLZ74_08550"
     CDS             605..1402
                     /locus_tag="PLZ74_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98412.1"
                     /translation="MRNGVLAAALWIGLAGGALSQEAAPVAVGEAETAGFSFGLTLDL
                     FSAYVWRGCVYNDEPVFQPAATLAYATERYGTFGLGLWGNLDLTDRNRKVSGGSFNEV
                     DYSLFHAFDVGGFTLEWGHFGYTFLKANGRDSYGNTKEVYLSVSHNNDLVTPFASLAY
                     DYDLLDGFYFNAGLNKEVALGDQLALGAEVSVGAGDDDYMTYMGVDEPGLMDFNFSVY
                     AIYALTDTLSVSAKLAWMSLLDGDARDAEPYRDEDLLWGGVSLNLAL"
     gene            complement(1416..3353)
                     /locus_tag="PLZ74_08555"
     CDS             complement(1416..3353)
                     /locus_tag="PLZ74_08555"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOR98413.1"
                     /translation="MTDYSKLLNPEQCEAAMAGEGPLLVLAAAGTGKTRTLVYRVAYL
                     VEQGQPPDGLLLLTFTNRAAREMLDRAKEVAGETVGNLWSGTFHHICNRFLRRFGDRL
                     GYPHDFTILDRDDSHSLIDQCIRETVRNRKEFPKKDVIASLIGRAANRACDIEDVLDS
                     TLTELRVDPTEILRVANLYAEKKRGMGAMDFDDLLVNGLRLLTEHEAVRVFYQSRFRH
                     VLVDEYQDTNLLQARMVDILADTHGNIMAVGDDFQCIYSWRGADFRNIMDFPKRWPTC
                     RMVKLERNYRSVPEVLEVANACIRGNPEQFQKTLRPTRKARNKPQVLFLRDGEEQAAA
                     LLTLIERYQEDGYRLRDMAVLYRAHYHSIELQIALGRARIPYVITSGIGVFEQAHVKD
                     VLAFLRVCVNRSDRLAFGRLLSLLDGVGEKTVESLWSKIGNTFDTQSPETRARLRAAM
                     RPAVREQWAAIDQLFQAYHSEKLNTAGGEIVQRFCDLFYHTLLYRRFDNADKREEDIR
                     ELALQIGKSPSVEAFLQEVALLTNVDHEYDGAEREAKERLHLSTVHQAKGLEWPVVFV
                     IWVTEGMFPSSRSVGEAENDAEERRLFYVAVTRAKDELVLCAPGMRRTRDGGVFFCKP
                     SRFIKEIPRGLTRERYGNRHV"
     gene            complement(3452..3781)
                     /gene="trxA"
                     /locus_tag="PLZ74_08560"
     CDS             complement(3452..3781)
                     /gene="trxA"
                     /locus_tag="PLZ74_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOR98414.1"
                     /translation="MAGTVLDLTDATFDGAVAQGVTLVDFWAPWCGPCKMQTPILETQ
                     VAPALAGRANVAKVNVDDAKELAVRFSIKSIPALLIFKDGQVVQQMVGLQRGDALVKA
                     VEAVLGA"
     gene            complement(3947..4564)
                     /locus_tag="PLZ74_08565"
     CDS             complement(3947..4564)
                     /locus_tag="PLZ74_08565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOR98415.1"
                     /translation="MRATHRNFWLLTALAAIGFLWVFGCPAGNFWGKITLTVCGLCTA
                     SIVVAGVPWRHTDLTLAEGLIGVAAAVALYGLFWAGYALACRLFDFAPQQVNAIYAIR
                     GAGHPWLIGALLLLVTSPGEELFWRGFVQRQAAKRWGATTGVVLGTTFYGAVHLVSGN
                     LMLVLAASVAGLFWCALYRWRGNLTACMVSHALWTVGIFLFFPIR"
     gene            complement(4576..5520)
                     /locus_tag="PLZ74_08570"
     CDS             complement(4576..5520)
                     /locus_tag="PLZ74_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOR98416.1"
                     /translation="MFPSENQTQPDPAACRPRAAAALLPWLTVPRFWSLTASTVPVTV
                     GAALAAHDGHFNGPLLALCLLSGWLLQIAANLLNTYGDFRSGVDTSAHPPTAPHLVNG
                     TLRPYHVLGAGVLCLLAGALAGARAAALSDWHLALFAVAGVAGAACYTTGPRFKYAGF
                     GLPVVALLMGVLMVTASYFAQTRTLTLPSLILSLPVTCLVGAILHGNDLRDMASDHRA
                     GIRTASLVLGSERAPPLFMALHILPHLILVAGIAGGLLPPLSGIALLALPFTLNALRT
                     CAIGFRTGDRTRIGRLEGLSAGIHFLFGTLLTLGLFLG"
     gene            complement(5905..6081)
                     /locus_tag="PLZ74_08575"
     CDS             complement(5905..6081)
                     /locus_tag="PLZ74_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98417.1"
                     /translation="MKIRNFPLAFQKNPSDAIPCVRGEIIDSDSDSDPDSDSDPDPDS
                     DPDPDLDETMKPET"
BASE COUNT         1180 a         1889 c         2004 g         1199 t
ORIGIN      
        1 caaacgccgc gcgatcagcc gcatcacgcg cgagtacgag cagaagcggg ccgcgctcgg
       61 caatccggtg tcgccaaccc ttttccgcaa ccccaagttt tatttcgcgg cgatcgtggt
      121 gttcgtgttt ctgggcatct tgctgtccaa caccacggac aaggcggtgc ggcggatgga
      181 ggtgaccccg cacatgcgcg cgatccgcaa ccttgatgtg ctggccgagg ctctgggccg
      241 ctaccacttc catacgggaa gcttccccga cgctgtgccg gggcttgccg cgctggtgcg
      301 cgatcccgct gtcccaaagt gggacgggcc ctacatccgt tttctgcgca acgacccttg
      361 gaacacgccc ttcgtttacg cgcctccggc cgggaacggg ttgccgacgc tcttttcctg
      421 cggggaggac cggatcccgc ataccccgga cgatcttttc cctgaccccg cgcgtttcga
      481 tcccggcaca gcgtggacca acggatgggt ttccgccgag caacggctgc ccggtgtaac
      541 ggtcctgcct tcttcccccg atggaaagcg gtgaggtcgc aataaggtta ggaaagagaa
      601 aaacatgaga aacggggtgt tggcggcggc gttgtggatc ggtctcgcag gcggcgcgtt
      661 gtcacaggag gccgcgccgg tagcggtggg agaggcggag acggcagggt tctcgttcgg
      721 tctgacgctg gatcttttct ccgcgtatgt ctggcgtggc tgcgtataca acgacgagcc
      781 ggtcttccag cctgccgcga cactggcgta tgcgacagag cggtacggga cgttcggcct
      841 aggcctctgg gggaatcttg atctgaccga ccgcaaccgg aaggtctcgg gcggcagttt
      901 caatgaagtc gattattcgc tgttccacgc gtttgatgtg ggcggcttca cgctggagtg
      961 ggggcatttc gggtacacct tcctcaaagc gaatggccgc gactcttacg ggaacacgaa
     1021 agaggtctat ctctcggtca gccacaacaa cgatctcgtg accccgtttg cgagtctggc
     1081 ctatgactac gacctgcttg acgggtttta cttcaacgcc gggttgaaca aagaggtggc
     1141 gcttggcgac caactggcac tcggcgccga agtctcggtc ggcgcggggg atgacgacta
     1201 tatgacgtat atgggggttg acgagccggg cctgatggat tttaacttct cggtgtacgc
     1261 catatacgcg ctcacggata ccctgtcggt cagcgcgaaa ctggcgtgga tgtcgctgct
     1321 ggacggcgac gcgcgcgatg ccgaacccta cagggatgag gatctgctgt ggggcggcgt
     1381 gagtctcaac cttgcgctct gagcggcgcg ggacctcaga cgtgccggtt gccgtaacgc
     1441 tcccgcgtca gtccgcgcgg gatttctttg atgaagcggg agggcttgca gaaaaacacg
     1501 ccgccgtccc gtgtgcgccg catgccgggc gcacacaaca ccagctcgtc ctttgcgcgc
     1561 gtgaccgcca cgtagaacag gcggcgctcc tccgcgtcgt tctcggcttc gcccacggag
     1621 cggctggacg ggaacatccc ctcggtgacc cagatcacga acaccacggg ccactccagc
     1681 cctttggctt ggtgcacggt gctgagatgc aaccgctctt tggcctcgcg ttcggccccg
     1741 tcatactcat ggtcgacatt ggtgagcagg gccacctcct gcagaaaggc ctccacgctc
     1801 ggacttttcc cgatctgcag cgccagttcg cggatgtcct cctcacgctt gtccgcgttg
     1861 tcgaaacgcc ggtagaggag ggtgtggtaa aacaggtcgc aaaagcgctg cacgatctcg
     1921 ccgcccgcgg tattcagttt ttcactgtga taagcctgga acagttggtc gatcgccgcc
     1981 cactgttcgc gcaccgccgg acgcatcgcc gcccgcaagc gtgcgcgcgt ttcgggggat
     2041 tgcgtgtcaa acgtgttgcc gatcttgctc cagagggact cgactgtttt ctcgcccacg
     2101 ccgtccagaa gactcagcag gcgtccgaac gccaggcggt cggaacggtt cacgcagacg
     2161 cgcagaaagg ccagcacgtc tttgacgtgc gcctgttcga acacgccgat gccggaggtg
     2221 atcacgtacg ggatgcgcgc gcgtcccagc gcgatctgca attcgatgga atggtagtgg
     2281 gcgcggtaga gcacggccat gtcccgcagc cggtatccgt cctcctgata ccgctcgatc
     2341 agggtgagca gggccgcggc ctgctcctcg ccgtcgcgta gaaacagaac ctggggtttg
     2401 ttgcgcgcct tgcgcgtggg ccgcagggtc ttctggaact gctcggggtt gccgcggata
     2461 caggcgttgg ccacctccag cacttccggc acactccggt aattgcgctc cagtttaacc
     2521 atgcggcagg tcggccagcg tttggggaaa tccatgatgt tccggaaatc cgccccgcgc
     2581 cacgagtaga tgcactggaa atcgtcgccc accgccatga tgttgccgtg cgtgtccgcg
     2641 agaatgtcca ccatccgggc ctgcagcaga ttggtgtcct gatattcgtc caccagcaca
     2701 tgccggaagc ggctctggta aaaaacgcgc accgcctcgt gttcggtcag aagccgcagt
     2761 ccgttcacca gcaggtcatc gaaatccatg gcccccatgc cgcgtttctt ctccgcgtag
     2821 aggttcgcca cgcggaggat ctccgtcgga tcgacccgca gttcggtgag cgtcgaatcc
     2881 agcacatctt cgatatcgca ggcgcggttg gcggctctgc cgatcaggga ggcgatcacg
     2941 tccttcttgg ggaactcctt gcggttccgc acggtctccc ggatgcactg gtcgatcagg
     3001 ctgtgcgaat cgtcgcggtc gagaatcgtg aaatcgtggg gatacccgag gcggtcgccg
     3061 aaacgccgca gaaaccggtt gcagatatgg tggaacgtgc cgctccagag gttcccgacc
     3121 gtctcgcccg caacctcttt ggcccggtcc agcatctccc gtgcggcgcg gttggtgaag
     3181 gtcagcagca acagcccgtc cggcggctgg ccctgttcga cgaggtaggc cacccggtaa
     3241 accaaagtcc gggttttgcc cgtgcctgcg gccgccagca ccagcagggg gccttcaccc
     3301 gccatagccg cctcacactg ttcggggttc agcagtttgg aataatcggt catgggaata
     3361 cacgccgccg ccgctgcgga cccggagagc ggaggtccgc aggcatactg gcggcagggg
     3421 agccggatcg cggaaagatt ggaccgggcg attaggcgcc gagcaccgcc tcgacagcct
     3481 tcaccagcgc gtcgccgcgt tgcagtccca ccatctgctg caccacctgg ccgtctttga
     3541 agatcagcag ggccgggatg ctcttgatgc tgaaccgcac ggccagttct ttggcgtcat
     3601 cgacattaac cttcgcgaca ttcgcccggc ctgccaatgc gggggccacc tgagtttcca
     3661 gaatcggcgt ctgcatcttg caggggccgc accacggggc ccagaaatcc accagcgtga
     3721 cgccctgtgc cacagcaccg tcaaaggtcg cgtccgtcaa atccaatact gtaccggcca
     3781 tatctgcctc ctatactccc caaccgcagg ggttcacatt tgagcggtat cataacgggt
     3841 ttccctgccc gatggcaaga acgcaaaccg cgagttcacg ggcatacggg cgcagccccg
     3901 tttcattcgg agaaggctca ggaggagcgg cgcgaaacct tgcgcgtcaa cggatcggga
     3961 agaacagaaa gatcccgacg gtccagagcg catgggagac catgcaggcg gtgaggttgc
     4021 cgcgccagcg gtagagtgcg caccagaaga gtccggccac gctcgcggcc aagaccagca
     4081 tgaggttgcc ggaaaccaga tgcacggcac cgtaaaacgt tgtgccgaga acgaccccgg
     4141 tcgtcgcgcc ccagcgcttc gcagcctggc gttgcacgaa accgcgccag aacaactctt
     4201 cgccgggact ggtcaccagc agcaggagag cgccgatcag ccacggatga cccgcgccgc
     4261 ggatcgcgta gatcgcgttc acctgttgcg gcgcgaagtc gaacaggcgg catgccagcg
     4321 cataaccggc ccagaagagg ccgtagagcg ccacggcggc cgccacaccg atgagcccct
     4381 cggcgagcgt caggtcggtg tgccgccacg gcactcccgc caccacgatt gaggcggtgc
     4441 agagtccgca cacggtcagg gtgatcttgc cccagaagtt gcctgcagga cagccaaaga
     4501 cccagagaaa cccgatggcc gccagagccg tgagtagcca gaaattccgg tgggttgcgc
     4561 gcatgaagag ttcctttatc cgagaaagag gcccagcgtg agcagggtgc cgaagagaaa
     4621 gtggatgcct gcggagagcc cctccaagcg gccgatccgt gtccggtcgc cagtgcggaa
     4681 ccctatggcg caggtgcgga gcgcattcag ggtgaacggc agtgcgagga gggcgatgcc
     4741 ggacaggggc ggcaacaaac cgcccgcgat cccggcgacc agaatcaggt ggggcaggat
     4801 gtgcagggcc atgaaaagcg ggggcgcgcg ttcggaaccc aaaaccagcg aggcggtccg
     4861 gatgccggcc cgatggtcgg atgccatgtc gcgcaggtca ttgccgtgca ggatcgcgcc
     4921 gaccagacag gtgaccggca gggagaggat cagactcggg agggtgaggg tccgggtttg
     4981 ggcgaaataa ctggctgtga ccatcagcac gcccatcaac agcgccacta ccggcaggcc
     5041 gaaccccgca tatttgaaac gcggtccggt tgtgtagcac gcggcacccg ccacgcccgc
     5101 caccgcgaaa agcgcgaggt gccagtcgct cagcgcggcg gcgcgtgcgc cggccaaggc
     5161 ccccgcaagc aggcaaagca cgccggcccc caagacgtga tatggccgca gcgtgccgtt
     5221 gaccagatgc ggggcggtcg gcggatgcgc ggaagtgtcc acgccggacc ggaaatcacc
     5281 gtaagtgttc agcaggttgg ccgcgatttg cagtagccat ccgctgagca ggcaaagcgc
     5341 cagaagcggc ccgttgaagt ggccgtcgtg ggccgcgagc gccgcgccga cggtgaccgg
     5401 gaccgttgag gcggtcaggg accagaaacg cggcacagtc agccaaggaa gaagcgcggc
     5461 ggcggcgcgg ggccggcaag ccgcgggatc cggttgggtc tggttttcag aaggaaacat
     5521 ggccgcagta tacgtgacgg attgccgtaa aagcaagagc gacgggatca atggttttta
     5581 gtgccgcatc cgccgatatt gtttcaccac gggacacttt cttgggtttc agggtttcaa
     5641 ggtttcatgg tttcatggtt tcaaggtttc aaggtttcat ccaaatcggg gtcgggatcg
     5701 ctatcggaat cgattgtttc accacgggac actttcttgg gtttcagggt ttcaaggttt
     5761 catggtttca gggtttcaag gtttcagggt ttcatggttt catccaaatc ggggtcggga
     5821 tcgctatcgg aatcggaatc gatcatttca ccacgggaca ctttttttgg tttcagggtt
     5881 tcatggtttc atggtttcag ggtttcaggt ttcaggtttc atggtttcat ccaaatcggg
     5941 atcgggatcg ctatcggggt cgggatcgct atcggaatcg ggatcgctat cgctatcgga
     6001 atcgattatt tcaccacgaa cacacgggat ggcatcgctc gggttttttt ggaacgccag
     6061 ggggaaattc ctgattttca tgaagtttca tgtgtttcac ggtttcatcc aaatcggaat
     6121 cgggatcgct atcgctttcg gaatcgattg tttaaccacg ggacactttc tttggtttca
     6181 gggtttcaag gtttcagggt ttctggtttc agggttgcat cctcacgccc ttgcctcggg
     6241 gacccgcatc cattacggtt gggctttctt cc
//
LOCUS       DAOSRB010000163         6248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11533_length_6248_cov_6.958824, whole genome shotgun sequence.
ACCESSION   DAOSRB010000163 DAOSRB010000000
VERSION     DAOSRB010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11533_length_6248_cov_6.958824"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..348
                     /gene="rpsP"
                     /locus_tag="PLZ74_08580"
     CDS             91..348
                     /gene="rpsP"
                     /locus_tag="PLZ74_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOR98418.1"
                     /translation="MVKIRLRRTGCKNTAAYRVVATDERSPRDGKFLEILGWYDPKLE
                     GENFKLNMERVAYWRSKGAQMSETVASMVRKAEKSAAAVTA"
     gene            366..1085
                     /gene="trmD"
                     /locus_tag="PLZ74_08585"
     CDS             366..1085
                     /gene="trmD"
                     /locus_tag="PLZ74_08585"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOR98419.1"
                     /translation="MGTPLLIDVLTVFPGMLRGFLEESMLNRAARMGAVVFRTVDLRD
                     FAHDARRTADDRPYGGGPGMIMKPELWFEAVETVRTPGARVILMTPAGNVFRQSRARR
                     LARERHLIFLCGHYEGIDERVREALVTDEVSIGDYVLTNGVLPAAVVVDAVVRLLPGV
                     LGGGEKATEDESFSDGLLEPPQYTRPAVYRGMAVPEVLQNGHHAATDAWRRSQALDRT
                     ARKRPDLLKAQSANGILEEKE"
     gene            1091..1453
                     /gene="rplS"
                     /locus_tag="PLZ74_08590"
     CDS             1091..1453
                     /gene="rplS"
                     /locus_tag="PLZ74_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOR98420.1"
                     /translation="MIAKAIDKINAEQVEKANAGKTAPEFKVGDSVKVFVKIKEGDKE
                     RLQAFAGTVIARDNKRGNTATFTVRRFSFGVGVEKVFPLNSPYIDRVEVERSARVRRA
                     KLYYLRKRTGKKARLREV"
     gene            complement(2169..2639)
                     /gene="rpsG"
                     /locus_tag="PLZ74_08595"
     CDS             complement(2169..2639)
                     /gene="rpsG"
                     /locus_tag="PLZ74_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOR98421.1"
                     /translation="MARRRRAVKRVQPADPKFNSELVARVIRALMKSGKKTTAERIVY
                     GAIEELKEKTGKDPIEVLDVAIANMKPKVEVKSRRVGGTTYPVPVEIRPARQLSLALR
                     WMTMYASSRRGTPMPKAVAAELIEAYNNQGNVIKKRDDTHKMAQANRAFAHYAW"
     gene            complement(2643..3026)
                     /gene="rpsL"
                     /locus_tag="PLZ74_08600"
     CDS             complement(2643..3026)
                     /gene="rpsL"
                     /locus_tag="PLZ74_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOR98422.1"
                     /translation="MPTINQLVRKGRSPLRKKSKSPALKACPQRRGVCLVVKTQTPKK
                     PNSALRKVARVRLTTGHEVSAYIPGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGCL
                     DSLGVAGRNRSRSKYGMKRQKKTES"
     gene            complement(3174..4463)
                     /locus_tag="PLZ74_08605"
     CDS             complement(3174..4463)
                     /locus_tag="PLZ74_08605"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98423.1"
                     /translation="MKMTRRDMMKQAGAVAAVGFPSIIPAKVLGQQAPSKMLTLACVG
                     VGGMGLSHISQLKAHRDRVRIVAVADVDIYHQRRAVRQIDEMNGDRSCRMYTDFREVT
                     RAPDIDLIAIATPDNWHALTAIDAIRHGKDVFVEKPLTLTVREGQALVSELRKHKRIG
                     QTGTMQRSSEAFHHAAELIRNGRLGKIRRIEVLIPANNRHVGATWKPEAVPEGLDYDF
                     WLGPAPYAPYTSQRTHYQFRYILDYAGGQTTNWGAHYLDIAQWALGMDESGPVEAEGH
                     GVFPSSGLFTAPTRVDVRYTYANGVTMRMRTRTDGVYDGNIIFYGDQGTLDVSRSKLK
                     SDPEGILKEKIGAGETRLYKSRNHFGNWIDCVKSRELPVSDLAIGHKTTNLCNIGNIA
                     MQLQRRLVWDPVREMFAGDDMANRMLTRPMRGPWSLA"
     gene            complement(4478..4990)
                     /locus_tag="PLZ74_08610"
     CDS             complement(4478..4990)
                     /locus_tag="PLZ74_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98424.1"
                     /translation="MSDKTQVSDGKRAATDPLAGQAADARYKGFNASADPWKVIGTLQ
                     YGEDRTLASTVERVVLDADASQYPGFEKKLLALATDPACTEAGYGFICRILSLIASPA
                     SVAALKDRLTDDNEVKAYLTRMVIEQVPGDEALAALREAAGRVKGREAEGLIGAVKAR
                     ESSRQGAKTF"
     gene            complement(5491..>6248)
                     /locus_tag="PLZ74_08615"
     CDS             complement(5491..>6248)
                     /locus_tag="PLZ74_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98425.1"
                     /translation="EEKLPVSVDTRTDFYSAYVWRGRALDKHGVVQPSVIATYDAQEY
                     GAFSAKVWSNWDLSQRSGDSKATRTGGGINVLNFTPSYSRAFGPVDVTVGNIFYTFPG
                     DRWPKNSNSTYELFTTVAYKNPVVTPSLSVYYDYRGVGGSFLEDNPLKDLYSRAALSK
                     SVAVTDRIRADGTALIGAGSSHYNNVRYCGSGEGFTDYLVSFALTYALTEALSLGGSL
                     EYTGLIGGAWGLDRHALSPDEIVRCGIHLRCVF"
BASE COUNT         1206 a         1964 c         1833 g         1245 t
ORIGIN      
        1 atgacatacc gcggaattcc gatttggaaa aatcccgctt gccgccgcta cccgcatctt
       61 ggtatggtat tacgttttac ggaggagtaa atggtcaaga tcagacttcg cagaaccgga
      121 tgtaaaaaca cggcggctta tcgcgtcgtg gcgaccgacg agcggtcgcc gcgtgacggc
      181 aagtttcttg aaattctcgg ctggtacgac cccaagcttg aaggggagaa tttcaaactg
      241 aatatggagc gcgtggcgta ctggcgttcc aaaggcgcgc agatgagtga gaccgtggcc
      301 agcatggtcc gcaaggctga aaaaagcgcg gcggcggtca cggcctgacg ggacggtggt
      361 ccgttatggg cacccccctt ttgatcgatg tgctgaccgt gtttccgggt atgctccggg
      421 gttttctcga agagagcatg ctcaaccggg cggcgcggat gggggcggtg gtttttcgga
      481 cggtggatct gcgggatttc gcgcatgacg cgcggagaac agcggatgac cggccttacg
      541 gaggcggccc cggcatgatc atgaagccgg agttgtggtt cgaggcggtg gagacggtgc
      601 gtacgccggg cgcgcgggtc attctgatga cccctgcggg aaacgtcttc cggcagagca
      661 gggcgagacg gttggcgcgt gagcggcacc tgattttcct ctgcggacac tatgagggca
      721 ttgacgagcg ggtgcgcgag gcgctggtga ccgatgaggt ctcgatcggc gactatgtgc
      781 tcaccaacgg ggtgttgccc gcggcggtgg ttgtggatgc ggtggtgcgg ctgctgcccg
      841 gcgtgctggg cggcggcgaa aaggcgacag aggacgaatc gttttcggac gggttgctgg
      901 aaccgccgca gtacacgcgg cctgcggtgt accggggaat ggcggtgccg gaggtgttgc
      961 aaaacggcca tcatgcggca acggacgcct ggcggaggag tcaggcactg gacagaacgg
     1021 cgcggaagcg cccggacttg ctgaaggctc agtccgcaaa cgggattttg gaagagaagg
     1081 agtagggatc atgattgcta aagcgattga caagatcaat gccgaacagg tcgagaaggc
     1141 caatgcggga aaaaccgcgc ctgagttcaa ggttggcgac agcgtaaagg tcttcgtgaa
     1201 aatcaaggag ggcgacaaag aacgcctgca agccttcgcc ggcacggtga tcgcccgcga
     1261 caacaagcgc ggcaatacgg cgactttcac cgtgcgccgc ttctctttcg gtgttggcgt
     1321 agagaaggtc ttccccctca actcgcccta catcgaccgt gtcgaggtgg agcggtccgc
     1381 ccgcgtccgc cgcgccaaac tgtattacct gcgcaagcgt accggtaaga aggcccgtct
     1441 gcgcgaagtg tgaccaggtc gggaattggg ctgataatcg gggcgatagc ggccccgatc
     1501 ccaatgctcc atgttggagg gaacgagcgt ctgcccgcct gggcaagcca atgcttgccc
     1561 ttcccgtttt cgtgcctgtt tggtgaatgc ctccgatccg cgttgcgggt gagaacgccc
     1621 gcgcacccgc ctcctgtctt cttccgtaat cgaaagggtg accccaattg gcatcgaggt
     1681 cgggggagaa caagacagga ttaacaggat tggagatgag cgtttgccat accgcgaaaa
     1741 cactccgcac ccccgcaatt gtctcatgtc agttgcgctg aaaaaggcgg gcagcgattt
     1801 tagagtccat gtcgaacaca gcattcggaa ccggtatcga cagaaggaca tcttcagcaa
     1861 agcgcggatg gttgctcccg tcctgactat aggcaagaat cgtctgaacc ggtgtgagtc
     1921 gtagaaagac tgcgagcgtt tcaggtgaca gtatctttgc ctttggacgc aacacgataa
     1981 actcagacga gccgacagca gggatggcac tccctgttcg taccactgca atttccttga
     2041 gataggaacc acgacgtccc catcccgcgc aaagctttta ctctcaagat tgcgagccac
     2101 tgcgtggccg caataaaaaa aagccatgcg ggcgtccgca tggctctaaa aatcgcacgt
     2161 gcagcgtctt accacgcgta atgcgcgaag gcccgattgg cctgcgccat cttatgcgtg
     2221 tcgtcgcgtt tcttgatcac atttccttga ttgttgtacg cttcgatcag ctccgccgcc
     2281 accgccttgg gcatcggcgt gccgcgccgc gagctggcgt acatcgtcat ccagcgcagt
     2341 gccaacgaaa gctgacgggc cggacggatc tccaccggca ccggataggt cgtgccgccc
     2401 acgcggcggg atttgacctc aaccttcggc ttcatgttcg cgatggcgac gtccagcacc
     2461 tcgatcggat ccttgccggt cttctccttc aactcttcga tcgcgccgta cacgatacgc
     2521 tccgccgtgg tcttcttgcc gctcttcatg agcgcccgga tcacgcgagc gaccaattcg
     2581 ctgttgaatt tcggatcagc gggctgcacc cgcttcactg ctctgcgccg tcttgccatg
     2641 gattaactct ccgtcttctt ctgacgcttc atgccgtatt tcgaacggct gcggttgcgc
     2701 ccggcaacgc ccaagctatc cagacacccg cgcacgatgt gataccgcac acccggtaaa
     2761 tccttgacgc ggccaccacg caccagcacc acgctgtgct cctgaagatt gtgcccctcg
     2821 cccggaatgt aagcggagac ctcatgaccg gtggtcaaac ggacgcgcgc aaccttgcgc
     2881 aacgccgaat tcggcttctt aggcgtctgc gtcttcacca cgaggcaaac cccgcggcgc
     2941 tgcggacacg ccttcagcgc aggcgacttg cttttcttac gcaacgggct tctgcccttg
     3001 cgcaccaatt ggttgatcgt cggcatcgga ttcgattcct tcccggattg gaaataaatg
     3061 gagaataaac ataccacagg aggcgaccgg cgtgcaagtg cgatttttga cgattttaca
     3121 gccggggcgc atcccccctt tctctggccc gatgccgtca cgggcaatca ccgctacgcc
     3181 agagaccacg gcccccgcat cggccgcgtc agcatgcggt tagccatgtc atcccccgcg
     3241 aacatctcgc gcaccggatc ccagacgagc cgacgctgga gttgcatcgc gatgttgccg
     3301 atgttgcaga gattcgtggt cttgtggccg atcgccagat ccgacaccgg cagctcgcgc
     3361 gatttgacgc agtcgatcca gttgccgaag tggttgcggc tcttgtagag gcgtgtctcg
     3421 cccgccccga ttttctcttt gaggatcccc tcgggatcag acttcagctt gctgcgcgac
     3481 acatcgagcg tgccctggtc gccgtagaag atgatgttgc cgtcgtagac gccgtcggta
     3541 cgcgtgcgca tccgcatcgt cacgccgttc gcataggtgt agcgcacgtc cacgcgcgtc
     3601 ggggccgtaa agaggccgct ggacgggaac acgccgtggc cttccgcctc caccggtccg
     3661 gattcgtcca tgcccagcgc ccactgcgcg atatcgaggt agtgcgcgcc ccagttcgtg
     3721 gtctgcccgc ccgcatagtc gaggatgtag cggaactggt aatgcgtgcg ctgcgacgtg
     3781 tagggcgcgt acggggcggg ccccagccag aagtcgtaat ccaacccttc gggaaccgcc
     3841 tcgggcttcc acgtcgctcc cacatgccgg ttgttagccg ggatcagcac ttcgatgcgg
     3901 cggatcttgc ccagccgacc gttccggatc agttcggccg catgatgaaa cgcctcggag
     3961 gagcgctgca tggtgccggt ctgcccaatg cgcttatgct tgcgcaactc ggacaccagc
     4021 gcctgcccct cccgcacggt gagggtcagc ggcttctcca caaacacatc cttgccgtgc
     4081 cggatcgcat cgatcgccgt gagcgcgtgc cagttgtccg gcgtggcgat cgcaattaga
     4141 tcaatgtccg gggcgcgtgt cacctcgcgg aaatccgtgt acatccggca tgaccggtcc
     4201 ccgttcatct cgtcaatctg ccgcaccgcc cgccgctggt gatagatatc cacatccgcc
     4261 accgccacga tgcggacccg atcccgatgc gccttcaact ggctgatgtg cgaaagtccc
     4321 attccgccga cccccacaca cgccagcgtc agcatcttgg agggcgcctg ctggcccaac
     4381 accttggccg gaatgatcga cgggaacccc accgccgcca ccgcacccgc ttgtttcatc
     4441 atatcgcgtc gtgtcatctt catgtctcgg atccccacta aaacgttttc gcgccctgcc
     4501 ggctgctctc ccgcgccttt acggccccaa tcaaaccttc cgcctcgcgc cctttgaccc
     4561 ggcccgccgc ctcacgcagc gccgccagcg cctcgtcccc cggcacctgc tcgatcacca
     4621 tgcgggtcag atacgccttc acctcgttgt cgtcggtcaa ccggtctttc aacgccgcca
     4681 cactcgcggg cgaggcgatc agcgacagta tccggcagat gaagccgtat cccgcctcgg
     4741 tacaggccgg gtccgtggca agggccagca gttttttctc aaaacccgga tattgcgacg
     4801 cgtccgcatc cagaaccacc cgctccaccg tggaggccag agtccggtcc tcgccgtatt
     4861 gcagcgtgcc gatgaccttc caagggtccg ccgaggcgtt aaatcccttg taccgggcat
     4921 ccgccgcctg tcccgccaac ggatccgtgg ccgcccgctt cccatccgaa acctgcgttt
     4981 tgtcactcat gcttccgtgt gcctcctact cttatccgaa tgaattggta tcatatcagc
     5041 atccgcactg cctgaccacc cgcttttttg cgggcccgcc tgcgggacgc attcctgatg
     5101 tgctgaacct acaaacaaag agcagttgaa ccttcacctc tctttggttt cagggtttct
     5161 cacgcgaaaa tacggaggtt ttggggggca tggacatccc gcccatgaaa cacgggcaaa
     5221 atacccgtgc cacaccgtcc gcgttactgg aaacgaaaac gaggacgagg acgtgaacgg
     5281 gaccccaatc ccgatagcga tacatcaggc tgggacggga ccagcgtcgg cttacccgga
     5341 cgagcagacc tcgtccctcc cctcttgatt tcatgaagtt tcatgtattt catggttcga
     5401 aaccggaaac cagtacaaac ggctcgatgg atgtgctggc gcatcaactc catcgttccc
     5461 ttgatcttct tggtgttgag cttgcgcgcc tcagaacacg caccgcaaat ggatgccgca
     5521 ccggacaatc tcatcggggg agagggcgtg gcggtcgagt ccccaggcac cgccgatcag
     5581 cccggtgtac tccagcgacc cgccgagcga gagcgcctcc gtcaacgcgt aggtgagggc
     5641 gaatgacacc agatagtcgg tgaacccttc gccggacccg caataccgga cgttgttgta
     5701 atggctggag cccgccccga tcagagccgt tccgtccgcg cggatgcggt ccgtcacggc
     5761 gacggacttg ctcagcgccg cacggctgta cagatcttta agcggattgt cctccaaaaa
     5821 gcttcctccc acgccacgat aatcgtagta gacactgagt gaaggcgtca cgaccggatt
     5881 tttgtaagcc accgtcgtaa agagttcata cgtgctgttg ctgttcttcg gccatctgtc
     5941 acccggaaac gtgtagaaga tgttaccgac ggtgacatcc acagggccga acgcccggct
     6001 gtaggacggc gtgaaattga gcacattgat ccccccgccc gtccgcgtcg ccttggaatc
     6061 ccccgaccgt tgcgagaggt cccagttcga ccacaccttg gcggagaacg ccccgtactc
     6121 ctgcgcgtca taggtggcga tcacgctcgg ctggaccaca ccgtgtttgt ccaacgccct
     6181 gccgcgccag acatacgccg agtagaaatc ggttcgcgta tcgaccgaca cgggcagttt
     6241 ctcctctg
//
LOCUS       DAOSRB010000164         6225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11578_length_6225_cov_9.686548, whole genome shotgun sequence.
ACCESSION   DAOSRB010000164 DAOSRB010000000
VERSION     DAOSRB010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11578_length_6225_cov_9.686548"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PLZ74_08620"
     CDS             <1..791
                     /locus_tag="PLZ74_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOR98426.1"
                     /translation="DIERFAHVAHANGVPLIVDNTFPTAVLCRPFDFGADIVVYSTSK
                     YLDGHAVALGGMIVDSGRFDWRAGGKFPGLTEPDPSYHGIVYADAFGPSAFIVKARVQ
                     LMRDLGAAPSPTNAFLLNLGMETLHLRMERHCRNAEAVAAFLEKHPAVAWINYPGLPS
                     SRDAALVRKYLPRGTCGVISFGVRGGRDAAMRFMDSLKLAAIVVHVADVRTSVLHPAS
                     TTHRQLTDAQLTATGIRPEMIRLSVGIEHPDDLIADIEQALAVC"
     gene            1000..1959
                     /locus_tag="PLZ74_08625"
     CDS             1000..1959
                     /locus_tag="PLZ74_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR98427.1"
                     /translation="MPSPRYLFGPVTSRRYGRSLGVDLAAPKTCTLNCRFCQLGPTSA
                     TTVTRTDSPPIGEVIGELRVWLASGQTAAFITASGSGEPTLHRRFGDLLRFARDETPC
                     RSLLLSNGTLFGLPEVRREAAQADVVKLSLHAWDQASFEQITRPHPSLRFETILNGYR
                     AFRQEFGGQIDLEVFLIHGVNDSPEQAQRIAALARQFGPDTLTLNTAVRPPADSGVTA
                     CPQDRMRALAPEFGPDTRISGAEPPTSPVAFTTEALASLVSRHPVSLNSLAATFALTE
                     EEMSARLAPLVAQRAIRLFETRGVVFAGPPVSEDQEPDDGPPQ"
     gene            complement(1921..2505)
                     /locus_tag="PLZ74_08630"
     CDS             complement(1921..2505)
                     /locus_tag="PLZ74_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOR98428.1"
                     /translation="MNLLLKLASVFFKIGLLTVGGGLAMIPIVQQEMVGRGWLTNRQF
                     LDILGIAQMTPGPMSVNMATFVGYRVVGTEYPGAVWLAVAGSVVATLAVCAPSLLCIN
                     LLGAFWMRNREHPCLVSVFTFLRPLVTGLVIHAALLLGVNCLWADPVREWLSQSPDVR
                     AGVLLVVAFLLTVLTRVSPVLVLLGGAIVGLLIF"
     gene            complement(2502..3101)
                     /locus_tag="PLZ74_08635"
     CDS             complement(2502..3101)
                     /locus_tag="PLZ74_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOR98429.1"
                     /translation="MTQETEDPRLAAIFTSFAHVGAFLFGGGYAMLPLIEREVVNRRG
                     WCRFEEMADYYAMAQLVPGVIAVNTAMLIGHRLRGFRGAAAAMLGVVCAPFFVILAYA
                     VAVDRLAGMPFLERALAGLRPAVAGMMLGIAYTMFNRTRRTRLGLTVCLVTTALVLGA
                     GVSAVAVILLGVTAGLVGYGVRKGLRGSVSDAENSGGQP"
     gene            complement(3098..3271)
                     /locus_tag="PLZ74_08640"
     CDS             complement(3098..3271)
                     /locus_tag="PLZ74_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOR98430.1"
                     /translation="MHTAIVAERGQVTIPKALRDRMGIRPSTVLSFSVKDGALIAVKA
                     VKEEPVSYVRGDE"
     gene            complement(3338..4594)
                     /locus_tag="PLZ74_08645"
     CDS             complement(3338..4594)
                     /locus_tag="PLZ74_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOR98431.1"
                     /translation="MKALAKYSMGIGDRFGRQGEAQLEAFKRFREQCGVEVVPVWNKS
                     DREHKTIGTRPESVRQEADAAAAKVGWTAPYHVDADHIRLATVDGFIASSDFFTLDVA
                     GYMGETTDEAEIYGFVARHEELIGSHAIEGLESPVTLTKPQMAEIVRGTLFAIRQAGR
                     LYRHIAEQKGSEPFITEISMDETTQPQTPEMLLVILAVVAEEQIPAQTIAPKFTGRFN
                     KGVDYAGDAALFGREFDADVCVVRYAVQHFGLPRNLKLSIHSGSDKFSIYPYVARTLQ
                     ARDAGVHVKTAGTTWLEEVTGLAVSGGEGLAIAKEIYLKALPRFDELCGPYATVLDIS
                     PERLPSPDEVASWNGGRFAASLRNVPGDAHFNPDFRQFIHVAYKVAAELGERYLTALD
                     ENRETVARHVTENLLTRHLKPIFRGA"
     gene            4777..5640
                     /locus_tag="PLZ74_08650"
     CDS             4777..5640
                     /locus_tag="PLZ74_08650"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR98432.1"
                     /translation="MTRGHGAWWGGAAVAGWLALATQAAPVTEKVDVIDLIRQKQTEV
                     HLGPKSEFSDKPSEIFQVRDDGMLHISGRGYGYLATQQAYQDYHLVVEFKWTGVACGK
                     RADRARDNGILVHAHGPHGSCGGTWIASVEAQIIEGGMGDIIVVSGKLPDGTPVTTSI
                     EAEIELDRDGEKRWKKGAPRQTVTGGRVNWEKRDEDWKDLLGFRGLHDLDAPVGDWNR
                     LEVIARGDTLRYFFNGRLVNEAFAVKPSAGRVCIQTEGAEMIVRRFELWPLDTFKEAW
                     KPVVQVTDAID"
     gene            5651..5857
                     /locus_tag="PLZ74_08655"
     CDS             5651..5857
                     /locus_tag="PLZ74_08655"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98433.1"
                     /translation="MHPRLGRVTAAGVEVDIRGTADPDSDVRINGVAATREGDAFSST
                     VLLRTGGNMVDVQAHKPGAEPTAP"
     gene            5957..>6225
                     /locus_tag="PLZ74_08660"
     CDS             5957..>6225
                     /locus_tag="PLZ74_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOR98434.1"
                     /translation="MAMTEALSISIEDYLEAIFYLVRENTVARSKDIAERLKVNRSSV
                     TGMLQALRDRGLVNYERYGFVTLTDEGKKVASRVVRRHEALRDFMV"
BASE COUNT         1251 a         2077 c         1791 g         1106 t
ORIGIN      
        1 tcgacatcga gcgctttgcg cacgtggcgc atgccaacgg ggtgcccctg atcgtggata
       61 acaccttccc gaccgccgtg ctctgccgcc ccttcgactt cggcgcggac atcgttgtct
      121 actccacctc caaatatctc gatggccatg ctgtcgcgtt gggcggcatg attgtggaca
      181 gcgggcgctt cgactggcgt gcgggcggca agttccccgg gctcaccgag cccgacccct
      241 cctaccacgg cattgtctat gccgacgcct tcggcccgtc cgcctttatc gtcaaggccc
      301 gcgtgcagct catgcgcgac ctcggcgcgg ccccctcgcc gacgaacgcc tttctgctca
      361 acctcggcat ggagacgctg cacctgcgca tggagcgcca ctgccgcaac gccgaggccg
      421 tggccgcctt tctcgagaag caccccgcgg tcgcctggat caactacccc ggtctgccga
      481 gctcaaggga tgccgccctc gtcaggaaat acctgccgcg cggcacttgc ggcgtgatct
      541 ccttcggcgt caggggcgga cgggatgccg ccatgcgctt catggacagc ctcaagctcg
      601 cggccatcgt cgtgcatgtc gccgacgtcc gcaccagcgt gctgcaccct gccagcacca
      661 cccatcgcca actcacggac gcgcaactca ccgcaaccgg catccgcccg gaaatgatcc
      721 ggctctcggt cggcatcgaa catccggatg acctcatcgc ggatatcgag caggccctgg
      781 cagtctgctg aaggtgagcg atcacgatgc cgcacccggg cgacaatgta caatgtgagg
      841 ataattggaa cgctgaacgt cggacaagga acgttgaatt tctcgttacg ccaacggtag
      901 ggacgcttcc ccaaggcgtc cgcagcggtt tcggggaaac caccccgcct ttttcaatac
      961 ggccttcgtg tgacagaatg gccagattat ggtactgaca tgccctctcc ccgttatctt
     1021 ttcggtcctg tcacctcccg gcgttacggc cgttcgttgg gtgtcgacct cgccgcaccc
     1081 aagacctgca ccctcaactg ccggttctgc caactcggcc ccacatccgc cacgacagtg
     1141 acccgcaccg attccccgcc catcggcgag gtgatcgggg agttgcgcgt ctggttggcg
     1201 tcgggacaga ccgcggcgtt catcacggcc agcggttcag gcgaacccac cctgcaccgc
     1261 aggttcggcg atctgctgcg tttcgcgcgc gacgagacgc cctgccgctc tctgctgctt
     1321 tccaacggaa ccctgtttgg cctgccggag gtccggcgcg aagcggctca ggcggatgtg
     1381 gtcaaactct ccctgcacgc gtgggatcaa gcctcctttg aacagatcac gcgaccgcat
     1441 ccgtcactcc ggttcgaaac gattctcaac ggataccggg cgttccgtca ggagttcggc
     1501 gggcaaattg atctcgaggt ttttctcatc cacggggtga acgacagccc cgaacaggcg
     1561 caacggatcg ccgctctcgc ccggcagttc ggtccggata ccctgacact caacacggcc
     1621 gtgcggccgc ccgctgactc cggcgtcacc gcctgtccgc aagaccgtat gcgcgcgctc
     1681 gcgccggaat tcgggccgga cacccggatc agcggcgcag agccgcccac ttcccctgtg
     1741 gcgttcacca cagaggcgct cgcgtccctg gtcagccgcc atccggtttc gctgaattct
     1801 ctggccgcga cgttcgcgct gacggaagag gagatgagcg cgcgcttggc cccgctcgtc
     1861 gcgcaacgcg ccatccgcct gtttgaaacc cgcggcgtgg tcttcgccgg ccccccggta
     1921 tcagaagatc aggagcccga cgatggcccc ccccagtaga accaaaaccg gcgaaacgcg
     1981 ggtcagaacg gtcaggagaa acgccacgac cagcaacacg ccagcgcgga catccggtga
     2041 ctgagacaac cattcgcgga ccggatcggc ccagaggcag ttcaccccca acaacagagc
     2101 ggcgtggatc accagaccgg tcaccagcgg acgcaggaag gtgaaaacac tcacaagaca
     2161 cgggtgttcc cggttccgca tccagaaggc cccgagcaaa ttgatgcaga gaagtgaagg
     2221 ggcgcacacc gccagcgttg cgaccacgga tccggccacc gccagccaga ccgcgccggg
     2281 atattcagta ccgaccacac gatacccgac aaacgtcgcc atgttgaccg acatggggcc
     2341 aggtgtcatt tgcgcgatgc cgagaatgtc cagaaactgg cggttagtca gccacccccg
     2401 tccaaccatc tcctgctgga cgatcggaat catcgccagc ccgcccccca cggtcaacag
     2461 accgatctta aaaaagaccg aagcgagttt gagcagcagg ttcacggctg ccccccgctg
     2521 ttttccgcat cagaaacgga tccgcgcaac cccttccgca ccccgtatcc gaccagcccg
     2581 gccgtgacgc ccaacaggat gacggccacc gcgctcactc cggcccccaa gaccagcgcc
     2641 gtggtcacca gacacaccgt caaccccaac cgcgtccggc gggtccggtt gaacatcgtg
     2701 tacgcgatac ccagcatcat ccccgccaca gcgggccgca accccgccaa ggcacgctcc
     2761 agaaacggca tccccgccaa ccggtccaca gccaccgcat acgccagaat cacaaaaaag
     2821 ggcgcgcaca ccacaccgag catcgccgcc gcggccccgc ggaatccacg cagacggtgt
     2881 ccgatcagca tcgccgtgtt caccgcgatc accccgggca ccaactgagc catcgcgtaa
     2941 taatccgcca tctcctcaaa gcggcaccac ccgcgccggt tgaccacttc ccgctcgatc
     3001 aacggaagca tcgcatatcc gcccccgaac aggaacgcgc ccacatgcgc gaaactcgtg
     3061 aaaatcgccg cgagtcgcgg gtcttccgtt tcctgcgtca ttcatccccc cgaacgtaag
     3121 agaccggttc ctctttcacc gctttcacgg cgatcagagc gccgtctttt acgctgaacg
     3181 acaaaacggt ggagggccgg atacccatcc ggtcacgcaa agcttttgga atggttacct
     3241 gtccccgttc ggcgacaatc gctgtgtgca tcattcacct ccatgaagta taggaatgct
     3301 tcgggataac caccctgttt ccgttcctat accgcgctca cgcgccgcgg aagatcggtt
     3361 tcaggtgcct tgtcagcagg ttttccgtaa catgacgcgc aacggtttcg cggttttcat
     3421 ccaacgccgt caagtaccgc tcccccaatt cagcagccac tttgtaggcc acatggatga
     3481 attggcggaa gtccggattg aagtgcgcgt cgcccggcac attccgcaac gaggccgcga
     3541 accgtccgcc gttccaggac gccacctcat ccggcgaggg caaccgttcc ggagagatgt
     3601 ccagcaccgt tgcgtagggg ccgcacaact catcaaagcg cggcagggct ttcaggtaga
     3661 tttccttggc gatcgccagc ccctcgccgc cggacacggc cagccccgtg acctcttcca
     3721 gccaagtggt gcccgccgtc ttcacatgca ccccggcatc gcgcgcctga agcgttcggg
     3781 ccacatacgg gtagatcgaa aacttgtcgc tgccggaatg gatactgagt ttcagattcc
     3841 ggggcagacc gaaatgctga accgcatacc gcacgacaca cacatccgca tcaaactcgc
     3901 ggccgaacaa cgccgcgtca ccggcataat ccaccccctt gttgaaccgc ccggtgaatt
     3961 tcggcgcgat cgtctgcgcc ggaatttgct cttcagcgac caccgccaaa atgaccagca
     4021 gcatctcggg agtctgcggc tgcgttgtct cgtccatcga gatctctgtg atgaacggct
     4081 ccgagccttt ttgttccgca atgtgccggt agagccgtcc cgcctgacgg atcgcaaaca
     4141 acgtaccgcg cacaatctcc gccatctgcg gcttggtcag ggtcacgggc gactccagcc
     4201 cctcgatggc atgcgacccg atcagttcct cgtgccgcgc cacaaacccg tagatctccg
     4261 cctcatcggt cgtctccccc atgtaaccgg ccacatccag agtgaaaaag tcggaagacg
     4321 cgataaagcc gtcgaccgtg gcgagacgga tatgatccgc atccacatga tacggtgccg
     4381 tccaccccac tttcgccgcc gccgcatccg cctcctgacg caccgactcc ggccgggtgc
     4441 cgatcgtctt gtgctcccgg tcggatttgt tccagaccgg caccacctcc accccgcatt
     4501 gctcgcggaa ccgcttgaac gcctcaagct gcgcctcgcc ctgacgcccg aaccgatcac
     4561 cgatccccat tgaatatttc gccaacgctt tcatctctct caccctcaac ccgccggaaa
     4621 agactcattc aatccgccgg aaacgtgata cagtgtcgca tatctttcca aacggggcaa
     4681 tcccgcccga tgacggaatc ggttcgtgac gataaggcat gctgtgttat actgttgaca
     4741 ccctgagttt gtttttcaaa tacgtgaggt gaaaaaatga caagagggca tggggcatgg
     4801 tggggtggtg cagcggtcgc cggatggctg gcgctggcga cacaggcggc accggtgacc
     4861 gaaaaggtcg atgtcatcga cttgatccgg caaaagcaga ctgaggtgca tctggggccg
     4921 aagtccgaat tctcagataa gcccagcgag atctttcaag tccgtgacga cgggatgctg
     4981 cacatcagcg gacgcggcta tggctatctc gcgacgcagc aagcctatca ggactaccat
     5041 cttgtcgttg agttcaaatg gaccggggtc gcctgcggca aacgcgccga cagggcgcgt
     5101 gacaacggca ttctggtgca tgcgcacggt ccgcacggca gttgcggtgg tacctggatc
     5161 gcgagcgtgg aggcacagat cattgaaggt ggcatgggcg acatcattgt ggtcagcggc
     5221 aagttgccgg acggcacgcc ggtcacgacc agcatcgagg ccgaaatcga gttggaccgc
     5281 gatggcgaga aacgctggaa gaaaggcgcg ccgcgccaga ccgtgacggg cggtcgcgtg
     5341 aactgggaaa agcgggatga ggattggaaa gacctgcttg gtttccgcgg tctgcatgac
     5401 ctcgatgcgc cggtgggcga ctggaaccgc ctcgaagtga tcgcgcgcgg cgacacgctc
     5461 cgctacttct ttaacggtcg tctcgtgaat gaggctttcg cggtgaaacc cagtgccggg
     5521 cgtgtttgca ttcagaccga aggtgccgaa atgatcgtgc ggcggtttga actctggccg
     5581 ctcgacacat ttaaagaagc gtggaaaccg gtcgtgcagg tgaccgacgc gatcgattaa
     5641 cggcgcggtg ttgcatccac gcctcgggcg cgtcacggcg gcaggcgtcg aagtcgatat
     5701 ccgcggcacg gccgatcctg acagcgacgt gcggatcaac ggcgtggcag ccacgcgcga
     5761 gggtgatgct ttcagcagca ccgttctgct gcgtaccggt gggaacatgg tggatgtgca
     5821 ggcgcacaag ccgggagcgg agccgaccgc accttgagaa ggtcgtagcc aaaaaagcaa
     5881 gttgacccct tgacatgtac ggatgtccgt agtatgtttg tctccacgaa ctctcagaaa
     5941 gaagggaccg agagacatgg cgatgacaga agcgttgagt atcagtatcg aggattattt
     6001 ggaggcgatc ttttaccttg tccgggagaa tacggttgcc cggtcgaagg atatcgcgga
     6061 acgcctcaag gtgaaccggt cgtctgtcac cgggatgttg caagccttgc gcgaccgcgg
     6121 gctggtgaat tatgagcgct acggttttgt cacgctgacc gatgagggga agaaggtcgc
     6181 cagccgcgtc gtgcggcggc atgaggcgtt gcgcgacttc atggt
//
LOCUS       DAOSRB010000165         6216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11600_length_6216_cov_6.328193, whole genome shotgun sequence.
ACCESSION   DAOSRB010000165 DAOSRB010000000
VERSION     DAOSRB010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11600_length_6216_cov_6.328193"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..1264
                     /locus_tag="PLZ74_08665"
     CDS             413..1264
                     /locus_tag="PLZ74_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOR98435.1"
                     /translation="MRYSRREFLQAGATGLAWAVAPRSAQAAARVAKQRKIQVGVQLY
                     SVRELCKKDLPGTLKAIKEMGYAGVEFAGYYGKNATEIKQLLDDNGLVMCGTHTGVDT
                     IRPQNLAKTIEFNLVTGNRYLMVPGMNAKDAQGWIDFAKLFSDAAVTAKAAGMYVGYH
                     NHQHEFKDKFDGKCKWEIFYTHASPLVCQQMDVGHVVSAGEDPVAWLKRFPNRSRTLH
                     AKEIYPGPGILGQVPEGKQGVKWDEVFAAAEADVTEWYIVESEADPNTLEKIRGCIDF
                     LKSKGRA"
     gene            complement(1503..3701)
                     /locus_tag="PLZ74_08670"
     CDS             complement(1503..3701)
                     /locus_tag="PLZ74_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98436.1"
                     /translation="MKRELIAGIAVAAVAAGAVELHVNPSAAPDGDGSAARPFATLVG
                     ARDGLRAARKAGTVTKDARVDIVLAPGDYVLAAGLALDAQDGGASADAPVTWKAARPG
                     TARIVGACRVPVAACRPVTDPAVLARLPEEAHGKVYEADVAALLPAELPPMAERFGAT
                     PTAPLLFINHKIATLARWPNDDFTSFSKSVDRGAVLKRHAGNSAEYGPGAFIYENPRA
                     KRWNFAQGVWMNGYWTHDWDNYSVRAAAYGVENGTNDVVRLATGVPYGVMGGTWGRKG
                     RRFYVFNLLDELDVPNEWYLDRVAKKLYFYPPNGTLAATDEVFLASLNEPLVKTAGSI
                     NHLRLQGLVFEYTYNTGLSLAGQGIQVLDCRVMCCGASGMSVHGNRNVIRGCEIAQVA
                     RVGLSVGGGDRRTLQRAETVVEDNHIHDFSMMQRTYAAGVSVSGCGITLRANVIHDAP
                     HMAVIYGGNEHVFEYNNVYRVLLETGDAGAYYTGRDWTTQGNVLRFNYTHDLGAEGEL
                     ANTMGFYFDDCDCGDAVYGNIFHNVARGILVGGGRDHPIRNNIFSRCLVGLSIDCRGM
                     TWKHWNSMEHGGPSWMLEDKAKALSYTNGVWAERYPRLADIMNDYPREPLYNPVEENI
                     FIDCTKQLLALDGKAPLERMASIARNVVVNTRGTNGVAWAAPDKRIASAFIILNGTTN
                     APCSFGFADAANGDFRFLPGAEILKVCPGFQVLPLDRIPADAGSKTTRDR"
     gene            complement(3728..>6216)
                     /locus_tag="PLZ74_08675"
     CDS             complement(3728..>6216)
                     /locus_tag="PLZ74_08675"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOR98437.1"
                     /translation="AVDGDMYTNVDEPSNPWVGYDYNAAIRISRIRVISRPDLISRFY
                     GAKVQIASDATFADATTVFTSSSANTTPGVPIEIVFDEPVTAKSVRVLSGTASGCRSS
                     VCEFEVVPADVPFKPVLTVVGYDDVTNFYPVLTWTVPAELHATKATLEYATGSAGPFT
                     ALATDVPIAITTFTNTAAKVGVLGYYRVTVDCNHPLFPGQSATSDAVTMGRCRRLDRS
                     WGNETTLLDGVTVMPATNGVYLSDHYPKAFDGNPDTFPDIFSGGTYSRGPVGLDFGEP
                     SWVTSFGYICRNDNWCYVRIKNTALFSADDGDVELTNKVQRSEKVTQASQDKTFYTQM
                     GTSLPDNGARKWFLYAPESGPSEFGGNVAELMFFGWTQSDLVAAGVVTAPTTLGFARS
                     ADGLSVDVGWNAGINVVQYRLERRVYGTNVWGTVATVAGTSLACNDNTVSQNIYEYRV
                     VSIGSNAVEAVSEPFTYTYYAPGTGTGLRGAVYAPYVANVAAMCQPEKYYELPTGPVN
                     LSVAKGAEIVAGSSITNNMRLLWRGKLVTPFTGAYTFTLVTTDGGALYIDKKSVANAW
                     TDGPKTTSGMVELTAGEHDIAVDARLQVSPVSCVLKWGGVVPDEVIPATQLIPAAEAA
                     TLTVDGWTQRTYNGQQYGNAYVTANNAYMISSSKEAPSDRNKLNATFLAKTARYSFDI
                     SARFRAILNGEAGIMARVPNGNFYAVEFKYDGSGFSYYGVRGVTNNSDTINMLLPWKL
                     TDTNDFSNVDYDLRLVYDSAVRTFHAYAKDNNKTEWNLIHTWHNDRQITGDYEYGFVV
                     HGASGNPVSSFTVTPLSIRLHRPGLTVLFK"
BASE COUNT         1290 a         1920 c         1810 g         1196 t
ORIGIN      
        1 taactcctaa ataaatccta catccgcgcg tagcgcggaa gacgcccgcg ctcccgactg
       61 cgcacacaca aaccacgcac taaactcgca taccccatac ccctgtcacg aacaaccaag
      121 ggttgcgaag cgttttcgcg gtgtggtaat gtgttgccgt ggcgaggcga ccgaggcagc
      181 aacggaagga tagcggcgcg gctgaaaacc gacaaagcct tgtatcggtc agccgcgcgc
      241 gtgttgaggt tgctggaaac acgaaatgtc tcatgtcggt tgcggggaga tgctatccga
      301 aaacccggcc tcccctgttt tacgattgtg cgaaaacccg cttttctgcc atactctctc
      361 catacgcctt cccggagtgg gacgaggcgt ctgacagaaa ggagagttta caatgcgtta
      421 ttcaagacgg gagtttctgc aggctggtgc aaccggcttg gcttgggcag tcgcgcctcg
      481 gtcggcgcag gcggcagcaa gggtggcgaa acagcgcaag attcaagtcg gggttcaact
      541 ctactcggta cgggagctgt gcaaaaagga tctgcccgga accctcaaag cgatcaagga
      601 gatggggtat gcgggcgtgg agttcgccgg atactatggc aagaacgcca cggaaatcaa
      661 gcagttgctg gatgacaacg ggctggtgat gtgcggcacc cacacgggcg tggacaccat
      721 ccggccgcag aacctcgcca agacaatcga gttcaacctc gtcaccggca accgatatct
      781 gatggtgccc ggcatgaatg ccaaggacgc tcaagggtgg atcgatttcg cgaagctctt
      841 ctcggatgcc gcagtcaccg ccaaagctgc gggcatgtat gtgggctacc ataaccacca
      901 gcacgagttc aaagacaaat tcgacggcaa gtgcaagtgg gagatcttct acacgcatgc
      961 gtcgccgttg gtctgccagc agatggacgt cggccatgtg gtgtcggccg gcgaggatcc
     1021 ggtggcgtgg ctgaaacgct tcccgaaccg ctcccgcaca ttgcacgcca aggagatcta
     1081 tccggggccc ggcatcctcg gccaagttcc ggaaggcaag cagggcgtga aatgggatga
     1141 ggtgttcgcg gccgctgaag cggatgtgac cgaatggtac atcgtggaga gcgaggcgga
     1201 ccccaacacg ctcgaaaaaa tccgcggctg catcgacttc ctgaagtcca aaggccgcgc
     1261 ataacccgtt ccggactgga acgcgtgaaa gcccctcccc gaccggctcc gccggatcat
     1321 cgagaggggt ttttcgttat cctgatcctg cacaacgcgg ttgatccaac aggccgtccg
     1381 caccgtcggt gtcgccgttg gcccatatca aaaccatctt cttttgcgcg atgtcccagg
     1441 cgtaaatcgc ggttctgctc tgcggcacgg cagcgacatt ggacgatgcc gcctatggga
     1501 tgctatcgat cacgagtcgt ttttgatcct gcatcagcag ggatacgatc cagagggagg
     1561 acttggaaac cgggacacac ttttaggatt tcggccccgg gaagaaagcg gaagtcaccg
     1621 ttcgccgcat cggcaaaacc gaaagagcag ggagcgttcg tcgtgccgtt gaggatgatg
     1681 aaggcggagg caatgcgctt gtccggcgcg gcccacgcga caccgttcgt gcctcgcgtg
     1741 ttcaccacca cattacgcgc gatggacgcc atgcgctcca atggagcctt gccgtcgagt
     1801 gcaagcagtt gcttcgtgca gtcgatgaag atgttctctt ccaccggatt gtagagaggt
     1861 tcccgggggt agtcgttcat gatgtccgcc agacgcggat accgctcagc ccacacgccg
     1921 ttcgtgtagg agagcgcctt ggccttgtcc tccaacatcc acgaggggcc gccgtgctcc
     1981 atggagttcc agtgcttcca cgtcatgccg cggcagtcaa tggaaaggcc cacgaggcaa
     2041 cgcgagaaga tgttgttacg aatgggatga tcccgcccgc cccccaccag gatgccgcgc
     2101 gcgacattgt ggaagatgtt gccgtacacg gcgtcgccgc agtcgcagtc gtcaaagtaa
     2161 aagcccatgg tgttggccag ttcaccttcc gcaccgaggt cgtgcgtgta gttgaagcgc
     2221 aacacattgc cctgcgtggt ccaatcgcgg cccgtgtagt acgcgccggc atcccccgtc
     2281 tccagcaaca cgcggtagac gttgttgtat tcgaaaacat gctcgttacc gccgtatatg
     2341 acggccatgt ggggcgcatc gtgaatcacg tttgcccgga gcgtaatgcc gcaaccgctc
     2401 acgctgacac cagccgcata ggtacgctgc atcatgctga aatcatggat gtggttatct
     2461 tccacaacgg tctccgcacg ctgaagcgtc cgacggtcgc cgccgcccac acttaagccg
     2521 acgcgggcaa cctgcgcgat ttcacagccg cggatcacgt tgcggttgcc atgcaccgac
     2581 atacccgacg cgccgcagca catcacacgg cagtcgagca cctgaatgcc ctgacccgcc
     2641 agactgagcc ccgtattgta cgtgtattca aaaacgagcc cctgcaagcg caggtggttg
     2701 atgctccccg ccgtcttcac aagcggttcg ttgagcgagg ctagaaacac ctcgtcggtc
     2761 gcggccagcg taccgttggg cggatagaag tagagctttt ttgcgacgcg gtcgaggtac
     2821 cactcgttgg gaacgtcaag ttcatccagc aggttgaaca catagaagcg acgccctttc
     2881 cgcccccatg tgccgcccat cacgccatac ggcacccctg tggcaagacg caccacgtcg
     2941 ttcgtaccat tttccacgcc gtacgccgca gcacggacgg agtagttgtc ccaatcgtgt
     3001 gtccaatatc cgttcatcca cacgccttga gcgaagttcc aacgcttcgc gcgcgggttt
     3061 tcgtagatga acgcacccgg cccatattcg gcgctattgc cggcatgccg cttgagcaca
     3121 gcccccctat ccacactctt agagaacgac gtgaaatcgt cattgggcca gcgcgcgagc
     3181 gtggcgattt tgtggttgat gaaaagaagc ggtgccgtcg gcgtcgcgcc aaaacgttcc
     3241 gccatcggcg gcagctctgc gggcaacaga gccgccacat cggcctcgta caccttgccg
     3301 tgcgcctctt cgggaagacg cgccaatacg gcgggatcgg tcacaggccg acacgcggca
     3361 accggcacgc ggcaggcacc gacgatgcgg gccgtaccgg gacgcgcagc cttccaggta
     3421 acaggcgcat ccgccgaagc gccgccgtcc tgcgcatcca gcgcaagccc cgccgcgagc
     3481 acataatcac cgggtgcgag cacgatatcc acacgcgcat ccttcgtgac ggtgccagcc
     3541 ttccgcgcag cgcgcagacc gtcccgcgcg ccgaccagtg tggcaaaagg ccgtgcggca
     3601 gacccgtcgc cgtccggcgc ggctgaaggg ttgacatgaa gttccacagc gccagcggcg
     3661 actgccgcaa ccgcaatacc tgcgatgagt tctcttttca ttgattccct tgttttcaaa
     3721 cgagcttcta cttgaagagg acggttagcc ccggccgatg caacctgatc gagagcggcg
     3781 tcacggtaaa cgatgagact ggattgccgg atgcaccgtg taccacaaac ccgtattcgt
     3841 agtcgcctgt gatctggcgg tcattgtgcc acgtatggat caggttccac tccgtcttgt
     3901 tgttgtcctt cgcatacgcg tggaacgtgc gcacggcgga gtcatacacc aggcgcagat
     3961 cgtagtcgac gttcgaaaaa tcatttgtat cggtaagttt ccagggcagc agcatgttga
     4021 tggtgtccga gttgttcgtg acgcctctta cgccgtaata agaaaatccg ctgccatcat
     4081 acttgaactc caccgcatag aagttgccat tgggaacacg cgccatgatg ccggcttcgc
     4141 cgttcaatat tgcccggaac cgcgccgaaa tgtcgaacga ataacgcgcc gtctttgcga
     4201 ggaaggtcgc attcaactta ttcctgtcgg aaggagcttc cttagaactg ctgatcatgt
     4261 atgcgttgtt cgcagtgacg taggcgttac catactgctg gccattgtag gtgcgctgcg
     4321 tccaaccatc caccgtcagc gtggctgcct cggcagcggg gatcaactgc gtggcgggaa
     4381 tcacctcgtc gggcaccacg ccgccccatt tgagcacaca ggaaacgggg gagacttgga
     4441 gccgcgcatc cacagcgata tcatgctcac cggcggtgag ttccaccatg ccgctcgttg
     4501 tcttcgggcc gtccgtccaa gcattggcca cgctcttttt gtcgatgtaa agtgcgccgc
     4561 cgtccgttgt cacaagcgta aacgtgtagg cccccgtgaa cggcgtgacc agcttgccgc
     4621 gccacaaaag gcgcatgttg ttcgtgatgc tgctgcccgc aacgatttcc gcgcctttcg
     4681 ccaccgaaag attgaccggg ccggtcggca gttcgtaata cttttccggc tgacacatcg
     4741 ccgccacgtt cgcgacatag ggggcgtaca cggctccgcg gaggcccgta cccgtaccag
     4801 gcgcgtaata cgtgtaagta aatggctcgg agaccgcctc caccgcatta gacccaatcg
     4861 aaaccacgcg gtattcgtag atgttctgcg aaaccgtgtt gtcattacag gcgagcgagg
     4921 tgcccgccac cgtcgccacc gtgccccaga cgttcgtgcc gtacacgcgc cgttccagac
     4981 ggtattggac aacgttgatg cccgcgttcc agcccacatc cacagaaagg ccgtccgccg
     5041 aacgcgcgaa accgagtgtc gtaggcgccg tcaccacgcc cgccgccacg agatcgctct
     5101 gcgtccagcc aaagaacatc agctccgcca cgttgccgcc gaattcgctc ggccccgatt
     5161 ccggcgcgta aaggaaccac ttgcgcgccc cgttgtcggg aagcgacgtg cccatctgcg
     5221 tgtagaacgt cttgtcctgc gaggcctgcg tcaccttctc cgaacgttgc accttgttcg
     5281 tgagttccac atcgccgtcg tccgccgaga agagcgccgt gttcttgatg cgcacatagc
     5341 accagttgtc gttgcggcag atgtagccga aactcgtcac ccacgacggc tcgccgaagt
     5401 caagccccac agggccgcgg gaataggttc cgccactgaa gatatcgggg aacgtgtccg
     5461 ggttgccgtc aaacgccttc gggtaatggt cactgagata cacgccattt gtggcgggca
     5521 tgacggtcac gccgtcgagt aatgtcgtct catttcccca agagcggtcc agcctgcggc
     5581 aacggcccat cgtgacagcg tccgatgtcg cggattgccc cggaaagagc ggatgattac
     5641 agtccacggt cacgcggtag taccccagca cgccaacctt tgccgccgtg ttggtgaacg
     5701 tcgtgatggc aatcggcaca tccgtcgcga gtgccgtgaa cgggccggcg gaaccggtcg
     5761 catactcgag ggtggctttc gtggcatgaa gctcagccgg cacggtccac gtaaggacgg
     5821 gatagaagtt cgtgacgtcg tcatacccca ccaccgtaag cacgggctta aacggcacat
     5881 ccgccggcac cacctcgaac tcgcacacgc ttgaccggca gccactcgct gtccccgaaa
     5941 ggacacgtac actcttggcc gtgacaggtt catcgaacac gatttcaatg ggcactcccg
     6001 gcgttgtgtt tgcggacgac gacgtgaata ccgtcgtggc gtcagcaaac gtagcgtcac
     6061 tcgcaatctg cacttttgcg ccgtagaagc ggctgatcag gtccggacgc gagatgacac
     6121 ggatacggct gatgcggatg gccgcgttat aatcatagcc aacccacgga tttgacggtt
     6181 cgtccacatt cgtatacatg tcgccgtcga cggcgt
//
LOCUS       DAOSRB010000166         6176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11671_length_6176_cov_6.113707, whole genome shotgun sequence.
ACCESSION   DAOSRB010000166 DAOSRB010000000
VERSION     DAOSRB010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11671_length_6176_cov_6.113707"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLZ74_08680"
     CDS             <1..200
                     /locus_tag="PLZ74_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98438.1"
                     /translation="KLRSLHEYLSRYPAPLAVRVYGGMYEEHPVQVGEKTCRLVNVPY
                     YATGLLHRYIAQAESTPEHGA"
     gene            276..1769
                     /locus_tag="PLZ74_08685"
     CDS             276..1769
                     /locus_tag="PLZ74_08685"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOR98439.1"
                     /translation="MKFEEPAEPLHTTQKDPFHLADSYARILAAPLSAVLEPGTPYRS
                     GFHWTERHLQCLWYDERYRPPSFPLPGGETATVLEPGEWNLEAGPDFLNATLLIHPGA
                     RRIRGDVEVHVRPSDWDAHRHSADPAYANVIAHVTWFEGPASGSLPAGAIPLSLVAAV
                     TARPDLSLDDIDIKAYPHAALPETPRPCEILMRNQPERVRALLVSAGQYRLRVKAERL
                     RARLEQSGDRTQVVYEEIMAALGYKHNQAPFRAVARLLPVAALSAPRESAFARLLGTA
                     RLLPQPDVAPDEEGRRVIRRLWDLWWRQEGPVLPDSISWRHHNVRPQNAPVRRLAAAA
                     GLFSGITTVLHDLDRLPREEGARWFAQATGQMAARAQWTFWDQRLTFASVPTPEYPVA
                     LLGASRASALLANIVLPLYAAEGTLPPDLLEHMPSEDLSSSMRLAALYLLGRDHNPAL
                     YAGNGLLQQGLLQIHHDFCLMARPGCEACALCAVLRNLSSESPDPLR"
     gene            1932..2429
                     /locus_tag="PLZ74_08690"
     CDS             1932..2429
                     /locus_tag="PLZ74_08690"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOR98440.1"
                     /translation="MACLIVMDGPLKGQRVTLDQPVTRVGRREGNDWVLPDGSVSGTH
                     CEIEKREDGFLVRDLGSTNGTKVNNETVKEAPLFRNDIILMGEIPVMIEGEDVPQGNK
                     AEPITIPRTTIVIQQNRPIEPPPKEFARRSNSNAIWIALIVLLLLVIGGMLYLFFTGS
                     SVSTQ"
     gene            complement(2433..3461)
                     /locus_tag="PLZ74_08695"
     CDS             complement(2433..3461)
                     /locus_tag="PLZ74_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOR98441.1"
                     /translation="MCKDDGIGQAVHADAAKGRGVCLFSGGLDSQLAVCVLREQGVHV
                     EGVVFESPFFRIEGAQRSAAKLNLTLHEIPFTADILELVQHPRHGFGGALNPCIDCHA
                     RMIQRAGEWMTRNGFDFVATGEVLNQRPLSQTRHSLAVVERDAALDGRLLRPLSALLL
                     EPTLPETLGLVDRTRLLGLNGRSRKPQMELAARYGLKEYPAPAGGCLLTEKGFCRKLT
                     DLQKREGLDDERLVRLLTIGRHFRLPGSAKKCVMGRDARENALLQKIVQPDDLWLHTV
                     DIPGPTVLIPLGADDTNDRMTAAALCAAYGDHQGRSRVTVRIHTANGIEDHRVVPMER
                     ERGAAWLC"
     gene            complement(3454..3987)
                     /locus_tag="PLZ74_08700"
     CDS             complement(3454..3987)
                     /locus_tag="PLZ74_08700"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOR98442.1"
                     /translation="MGWFGMAAVLTVCTFVPGLEARASIPLAFFHTGIRQCMGLPVAL
                     TICLLSNILVGVLTFWLMGPVVQMFRRWAWFERVVWPRFERTRQKLHPYVERYGEWGL
                     AIFIGVPLPGTGAYTGAFGAFLLGMDRRRFLVANALGVLIACLAVSVLCLLIDHGFVA
                     EQSLLRKLFLKSVNLNV"
     gene            4059..4880
                     /locus_tag="PLZ74_08705"
     CDS             4059..4880
                     /locus_tag="PLZ74_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOR98443.1"
                     /translation="MAFRLSTLNLTGLFGVCAFTCLHAQLTAMPIRQPGATLDTSIRN
                     EADRAADQAAAWLISRQRPDGSWGISNRTVRSTALALLALQTSPRLTVSRSDACVRAA
                     LYLDRTSRLPLPDLETHAWRLLALAAALPHDDPERTSCLQRLAHQTEPILADAAESPR
                     RFWSEAMSAAGLTNSTPAAYPDDSARLACLANRWPPEDTPDHAVLWRCARLVNRAGKG
                     QLVRGESVLDWRKDLAVRLIRTQRRAPEAGGYWAASSTHDACEETAFGMLILLEL"
     gene            4908..5714
                     /locus_tag="PLZ74_08710"
     CDS             4908..5714
                     /locus_tag="PLZ74_08710"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOR98444.1"
                     /translation="MKLIRFLTPSSPTPLTGILHEDGSAEPIEGDLFATWEPTGRRIE
                     ITSLLAPVRPPLILAIGLNFRAHAAETGQPIPDFPVLFMKSPQAVLDPGAPIRIPTRL
                     PSTQVDYEAELVVIIGRTCTNATHENALSYVLGYTCGNDVTARDWQKSFGGSQWCRSK
                     SFDTFAPLGPCLVTREEIPDPQNLEVRAIVNGETRQQANTSDHIFSVTDLIVFLSGST
                     TLPAGTALFTGTPSGAGFACTPPRYLAPGDTVTIDIRGIGELTNPVVREA"
BASE COUNT         1207 a         2106 c         1714 g         1149 t
ORIGIN      
        1 gcaagctccg gtcgctgcat gagtatcttt cgcgctatcc cgcgccgctg gcggtgcgtg
       61 tctatggcgg gatgtacgag gaacacccgg ttcaggttgg cgagaagaca tgccgcctgg
      121 tcaatgtgcc gtattatgca acagggctgc tgcaccgcta tatcgcccaa gccgaatcca
      181 ctccagaaca tggcgcatga cctccgcaca cccctgtcac gaaccgatta ccaatttttg
      241 tttacgaaaa gaaacgatac tgttatcttt tgtgtatgaa atttgaggaa ccggcggagc
      301 cccttcatac cacgcagaaa gatcctttcc atctcgctga ttcctacgcc aggatactgg
      361 ctgctccgct gtccgccgtt ctcgagcccg gcacccccta ccgttccggc ttccactgga
      421 ccgagcgcca cctgcagtgc ctctggtacg acgaacgcta ccgtccaccc agtttcccgt
      481 tgcccggcgg cgaaaccgca accgtgctgg aacccggcga atggaatctg gaggccggcc
      541 ccgattttct gaacgccacg ctcctgatcc acccgggagc gcgccgcatc cgcggcgatg
      601 tcgaagtgca tgtccgtccc tccgattggg acgcgcaccg ccacagtgct gaccccgcgt
      661 acgcgaacgt catcgcgcat gtcacctggt tcgagggacc ggcatccggc tccctgccag
      721 caggggccat ccccctctcc ctcgtcgcag ccgtgacggc gcgccccgac ctctcgctgg
      781 atgatatcga catcaaagct tacccgcacg ccgcgttgcc ggaaacgccg cgcccctgtg
      841 aaattttgat gcggaaccaa ccggaacggg tcagagccct gcttgtctca gccgggcagt
      901 accgcctgcg cgtcaaagcg gaacggttac gcgcccgctt ggaacagagt ggtgaccgca
      961 cacaggtggt atacgaggaa atcatggcgg ccctcgggta caaacacaac caagcgccgt
     1021 tccgtgcggt cgcccgcctc ctgcccgtgg ccgcgctcag tgctccgcgc gaatcggctt
     1081 tcgcgcgttt gcttggcacc gcgcgccttc tcccccaacc ggatgtcgct ccggacgagg
     1141 agggccgccg cgtcatccgc cgtctctggg acctgtggtg gcggcaggag gggcccgtgc
     1201 tgccggattc gatctcttgg cgtcaccaca atgtgcggcc gcaaaacgcg ccggtccgtc
     1261 gcctcgcggc agcagcggga ctctttagcg gcatcaccac cgtgctgcac gatctcgacc
     1321 gcctgccgcg cgaggaggga gcgcgctggt ttgcccaagc gaccggacag atggccgcac
     1381 gcgcccaatg gaccttctgg gatcaacgtc tcaccttcgc ttcggtgccc acgcccgagt
     1441 atccggtcgc gttgctgggc gcctcccgcg catccgccct tctcgccaac atcgtgctgc
     1501 cattgtacgc ggcggagggt acgctgccgc cggatctcct cgagcacatg ccctccgagg
     1561 acctctcgtc ttccatgcgg ctcgcggcac tttacctgct cggacgcgac cataatcccg
     1621 ccctgtatgc cggcaacggt ctgctgcagc agggtttatt gcagatccac cacgatttct
     1681 gcctgatggc ccgtcccgga tgcgaagcct gtgccctgtg tgctgtattg cggaatcttt
     1741 cctcggaaag tcccgatccg cttcgatgaa acaaggaggt ttgcgtccca tagctattgc
     1801 cgcctcccca acaggcggag ttctgttttc ctcgcgtaat gaatttgctt gtttcatttt
     1861 gatacgcggc attgacttcc gacggcggga ttattaagct atagatcaat tttccattaa
     1921 aggtgcgtga tatggcttgc ctaatagtga tggacggccc gttgaaaggg caacgcgtaa
     1981 cgttggacca gccggtgact cgagtcggac gcagggaagg gaacgactgg gtgttgccag
     2041 acggctcggt atccggaacg cactgtgaga tcgaaaaacg ggaagacggt tttctcgttc
     2101 gagatttggg ttccaccaac ggcaccaaag tcaacaacga aacggtcaaa gaagcgccgt
     2161 tgttccgcaa tgacatcatt ctgatgggtg agatccctgt gatgattgaa ggcgaggatg
     2221 tgccgcaggg taacaaagcg gaacccatta cgattccccg caccacgatc gtcattcagc
     2281 aaaaccgccc catcgagcct ccgcccaagg agttcgcccg gagatcaaac tccaacgcca
     2341 tctggatcgc cctgatcgtc ctgctgttgc tggtcatcgg cgggatgctg tatcttttct
     2401 tcaccggctc gtcggtgtcg acgcaatgat cctcagcata gccacgccgc accccgttcc
     2461 cgttccatcg gcaccacacg atgatcctcg atgccgtttg ccgtatgaat gcggactgtg
     2521 acgcgggagc gtccctgatg gtcgccatac gccgcacaca aggccgccgc tgtcatccgg
     2581 tcgttcgtgt cgtccgcgcc cagcgggatc aacaccgtcg gaccgggaat atccaccgtg
     2641 tgtagccaga ggtcgtcagg ctgaacgatc ttctgcaaca gggcgttttc ccgtgcatcg
     2701 cgtcccatca cacatttttt cgcgcttccc ggcagccgga aatggcgccc gatcgtcagt
     2761 aagcgtacca aacgctcgtc gtccagcccc tcccgttttt ggagatccgt cagtttacgg
     2821 cagaacccct tctccgtgag cagacacccg cccgcaggtg cgggatactc cttgagtcca
     2881 taacgcgcgg ccagttccat ctgcggtttg cgcgaccggc cgttcaaacc cagcaggcgg
     2941 gtccggtcca caagccccaa tgtttccggc agcgtcggtt ccagcagcag cgcactcaac
     3001 ggcctcaaca accgcccgtc caaagcggca tcccgttcga ccacggcgag cgagtgccgg
     3061 gtctgggaca ggggacgctg gttcagcacc tcccccgtag ccacgaaatc aaacccgttc
     3121 cgcgtcatcc actccccggc gcgctggatc atccgcgcat ggcagtcgat gcaggggttg
     3181 agcgcaccgc cgaatccgtg acggggatgc tgaaccagtt ccaaaatatc ggcggtgaag
     3241 ggaatctcat gcagtgtcag gttcagtttc gcggcactcc gctgcgcgcc ctcgatccgg
     3301 aaaaaggggc tttcgaaaac caccccctcc acgtgcaccc cctgctcgcg caacacgcac
     3361 acggcaagtt gactgtcgag tccgcccgaa aacagacaga ctccgcggcc tttcgcggca
     3421 tccgcatgca cggcctgccc gattccgtca tccttacaca ttgaggttca cgcttttcag
     3481 gaagagtttc cggagcagac tctgttccgc cacgaacccg tggtcgatca acaggcacag
     3541 gacgcttacg gccagacagg cgatcagcac acccagagcg tttgccacca gaaaacggcg
     3601 ccggtccata cccaatagaa acgcgccaaa cgcgccggtg tatgcgccgg ttccgggaag
     3661 cgggactccg ataaaaatgg ctaaccccca ctcgccgtaa cgttcgacat acggatgcag
     3721 cttttggcgg gtgcgctcaa aacggggcca gaccacacgt tcaaaccacg cccagcgccg
     3781 gaacatctgc accacgggac ccatcagcca gaaggtcaac acccccacca gaatgttcga
     3841 gagcagacaa atggtcagag caaccggcaa ccccatgcac tgccgtatcc ccgtatgaaa
     3901 aaacgccagc ggaatcgacg cgcgcgcttc caatcccgga acaaacgtgc acaccgtcaa
     3961 aaccgccgcc attccgaacc agcccatcct ataacaccct ttccttccgc ccggcattgg
     4021 gctaaactat tctctttcaa aaggaaggag cataaacgat ggcctttcgc ctgtccaccc
     4081 tgaacctgac cggactgttc ggcgtgtgcg cgttcacctg cctgcacgcg caactgactg
     4141 ccatgccgat ccgtcagccg ggtgcgacac tcgacacctc gatccggaat gaggccgacc
     4201 gggccgcgga ccaggctgca gcatggctga tctcgcggca acggcccgac gggtcctggg
     4261 gcatctccaa ccgcacggtg cggagcacgg cactcgcgct gctcgcgctc cagacttccc
     4321 cgcgactgac cgtctcccgt tctgatgcct gtgtgcgcgc cgcgctttat ctggatcgca
     4381 cgtcacgcct gcccctgccg gacctcgaaa cccacgcctg gcgtcttctg gccctcgcgg
     4441 ccgccctgcc gcacgacgac ccggagcgca cgtcgtgtct ccaacgcctc gcgcaccaga
     4501 cggagcccat cctggctgat gcggcggagt ctccgcgccg cttctggagc gaggccatgt
     4561 ccgcagccgg tctgaccaac agcacccccg ccgcataccc ggacgattcc gcgcggctcg
     4621 cgtgtctggc gaaccgctgg cccccggaag acacgcccga tcacgccgtt ctctggcggt
     4681 gcgcgcgcct ggtcaaccgt gcggggaaag gccaactggt acgcggcgag tcggtcctgg
     4741 actggcgcaa agatcttgcc gtgcgcctga tccgcaccca acgccgcgcg ccggaagcgg
     4801 gcggatattg ggccgcctcc tccacgcacg acgcttgtga ggagaccgct ttcggaatgc
     4861 tgattctgct ggaactctaa ccctcttcat ctccgagaac ccggaatatg aaactcatcc
     4921 gttttctcac tccctcctct cccacccccc tcaccggcat ccttcacgaa gacggttcag
     4981 ccgaaccgat cgaaggcgac ctcttcgcaa catgggagcc taccggacgc cgcatcgaaa
     5041 tcacctccct gctcgcgccg gtccgcccgc cgctcatttt ggcgatcggg ctgaacttcc
     5101 gggcgcatgc cgcagaaacg ggacagccga ttcccgattt tccggtgctg ttcatgaaaa
     5161 gtccccaagc cgtgctggat cccggcgcac cgatccggat tcccacgcgg cttcccagca
     5221 ctcaggtgga ttacgaagcc gaactggtgg tcatcatcgg acgaacctgt accaacgcaa
     5281 cgcatgaaaa cgcgctgtcg tatgtattgg ggtatacgtg cggtaatgac gtgaccgcac
     5341 gggactggca gaaaagtttc ggcggcagcc aatggtgccg cagcaaatcc tttgacacct
     5401 tcgcaccgct gggcccctgt ctcgttacgc gcgaggagat ccccgacccg caaaatctgg
     5461 aggtgcgggc gatcgtcaac ggcgaaacac gtcagcaggc caacacctca gaccacattt
     5521 tcagcgtgac ggatctgatc gtgtttctaa gcggcagcac aaccctgccc gcaggcacgg
     5581 cactcttcac cggcacgccg tccggagcgg gattcgcgtg cacgccgccc cgctatctcg
     5641 cgcccggcga taccgtgacc atcgatatcc gcggcatcgg cgaactcacc aatcccgtag
     5701 tgcgcgaagc gtgagccgat aacctgctcg tccgggcaag cagacgctta cttaccggcg
     5761 gcacggtttc ggcggtccgg ctccgcgcct atggtgcgta actgtggacg tcccgccata
     5821 ccggcaggga ctgtcttcac tcagaccaac aaagcggtgg cggcgacaga atcccgccgc
     5881 ctcactactg aacccaaaag gagcaattga acgggtagaa ttccagcaaa gccccgtaaa
     5941 cactgggttt tacggtgcgc ccgacgctct ttttgaactc ttcgtcccag cccttcgtcg
     6001 caagcgcaat cgacggagtt ttggagaccg gtgcgtatgc gggactaagg cgtccctgtc
     6061 gatcgtcgcg ggtcgaatgt cgcgggtcgc gggtcgacgt tcccgatagc gatagcgata
     6121 ccgatcccga ccccgacccc gatttggatg aaaccatgaa accagaaacc cagaaa
//
LOCUS       DAOSRB010000167         6171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_11688_length_6171_cov_5.569326, whole genome shotgun sequence.
ACCESSION   DAOSRB010000167 DAOSRB010000000
VERSION     DAOSRB010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11688_length_6171_cov_5.569326"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLZ74_08715"
     CDS             complement(<1..623)
                     /locus_tag="PLZ74_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98445.1"
                     /translation="MKKRMVMLAVGLSIVPGIVRAATYIYDSGTNEVASLLGSLAQGD
                     EVKILNAGTRVISTASDDLVSKNDVSILIDGGWLEETATQAGRAGGLCNSLGVTGLSL
                     DIVNGGTYHVTTPNRGFFKLGGTGSNIRVEGGSLLLSDITEYNWGSGFGNAKDCTIAL
                     TNATMTAFVFRFGLDSKKATGCRLLVHDSTAQATGYCNTFNVLVQRM"
     gene            complement(739..2370)
                     /locus_tag="PLZ74_08720"
     CDS             complement(739..2370)
                     /locus_tag="PLZ74_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOR98446.1"
                     /translation="MRFALADWLVTVAFFVVLLAIPAFANRRRARTSSDFFQSGRSMP
                     WWLIGISMVAAMTSTNSANLFTQLIRENGLAGNWVWWSFLTGGVLTVFVYAKLWHRSG
                     ATTDIAFYELRYSGKPAAFLRAFRAVYLGVIYNVIVMATVLLGAVKLGAVLFGVPVWT
                     ILAVTAVASIAYSMIGGIRGTIYADFYLFTLIMIGAVAVTAFALSQPEVGGWAALMQN
                     AAIRPKLDFLPRFDDLDTAIAVFIVPVAIQWWNVWYSGSEPGGGGYIVQRMLTAKTPN
                     HALGGTLFAQIVQYALRPWPWYITAFASILVFPDLASIRAAFPDVDPALVGNDMAYPA
                     MIKFVPAGWLGLVAASLMGALFSTIAAHLSMGANYVANDVWVRFVRPQADGRELIRVA
                     RWTSVGLMLAGCLLVPFIADAKAGFDLMVMVGAGTGAVFLLRWFWMRINAWTEIAAMG
                     ASILCACFFQLVWPSFAVPLVFWKRLLLTIGVTTVVWLAATFLTRPEKPETIARFRSL
                     VRADGRDVGRGVLLTFLVSLAIFVFMWAIGVILCR"
     gene            complement(2380..3345)
                     /locus_tag="PLZ74_08725"
     CDS             complement(2380..3345)
                     /locus_tag="PLZ74_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98447.1"
                     /translation="MKHELLKLAKECGADLVGVAPASRFPADHKIFRIYPQVKSVIGL
                     AFRCLRGSYRGTEEGTTYYQYTTMSVENMEETVMPIALVRLANRIESKGFTAVPQRRH
                     QTIMEAEDGMNPEVAYDAIARGRVEEPQFDFTDAAVRCGLGERGFHGALLTDDFGPMQ
                     RTCFILTDAELEPDQVVTPHLCDRCGACVKGCPGKCIDPESGAIDPWRCAVYYNGANG
                     TKNPFMPPEAFPDFEDRLAIIAGEAEIDSEKAKRILDAIYFYPPAQHSYTCSICGRAC
                     DVACYCHLEKKGVLKRTFKTPFRTRPEWKFQLDDFAPESVNARQE"
     gene            complement(3342..4130)
                     /locus_tag="PLZ74_08730"
     CDS             complement(3342..4130)
                     /locus_tag="PLZ74_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98448.1"
                     /translation="MNRETIEQVARELGAAMFGVGDLKLFEGEDPRHDPKQVCPKAQA
                     IIGFGIPVPKGLYKTMKDESQFYAYTTLGVKAIDEEYFDIFLFKMASIIEDAGYDACL
                     QRTTPNLRVKGDKTANPEMIGVYELAHAEAVEPGKPPPDVMIDFGKAARACGIGMPGR
                     YGRVINREYGPFMRYAFIITDALLETNAPYADDLCAGCDACVKACDAGCIDVEKGLDS
                     WKCWEHYNKARGYSLPRTQWGYQACLCGRKCDVACWEHIKGVKL"
     gene            complement(4150..5235)
                     /locus_tag="PLZ74_08735"
     CDS             complement(4150..5235)
                     /locus_tag="PLZ74_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98449.1"
                     /translation="MLSAKRIKEIAKKAGADNCGIAPISRMSDAPDEMNPTFLFPNCK
                     SMVGLVFRIPRGVQRGIEEGTQFYQYPSMAYGGINEILAPATLYHVGKAIEDAGYEAF
                     VYRNTGARGCVSDMDGSEDAKLSPEEQIEVSENAKDSTAHHRSVDFTRATRPGENVPP
                     DLQFQFRICAVACGLGEIGWSKMLLTPEFGPLQRVAFLFTDAEFDEYDEIYNGKPLCR
                     RCGACVRECPGNCIPEIKSGKKCTASIGDYKMEWGDIDMWKCYAFYTHAGRYYNPFVP
                     KEVWDENKDGDLDLFAGKTDASEQEIMKVYRALAKYFPSWVGYNMAKCGGCIRACVEM
                     LEKPGGCLHGRFKNPLRTRPKAWKMDR"
     gene            complement(5237..6094)
                     /locus_tag="PLZ74_08740"
     CDS             complement(5237..6094)
                     /locus_tag="PLZ74_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98450.1"
                     /translation="MKFSVGYQDDAAWIEAIIARKDVMHDVYFAASGWASGRAVIRSE
                     DRLLDDLGRMAEAGLPLHLLFNATCYGGESLAKSLFSHIGETVERFAADGSLEGVTTT
                     SPLIAKFVKDNFPELKTRASVNMEIGTIEGMEYLADVFDGYYLKRELNRDLDAVRAAK
                     TWCDAYGKELFILANSGCLNHCSAHMFHDNLVAHETEIAQKDNGYVYRGMCWEWLAKP
                     ENRAKWMERTNWIEPESVTKYEGLCTAMKLATRINAHPVRILESYATGSHLGEIKGLL
                     EPDHSALWR"
BASE COUNT         1337 a         2007 c         1639 g         1188 t
ORIGIN      
        1 aacatcctct gaactaaaac gttgaaggtg ttgcaatagc cggtagcctg tgcggtcgaa
       61 tcatgcacca gcagacggca ccctgtcgcc ttcttgctgt ccagaccgaa cctgaagacg
      121 aacgccgtca tcgtcgcatt cgtcagggcg atcgtgcaat ccttggcatt gccgaaacca
      181 gacccccaat tgtattccgt gatgtcgctg agcaggagcg aaccgccttc cacccggatg
      241 ttcgatcccg tgccgccgag tttgaagaac cccctgttcg gtgttgtcac atggtaggtt
      301 cccccgttga cgatatcgag ggacaggccc gtcacgccca atgagttgca caggccaccc
      361 gcccgccctg cctgagtggc cgtctcctcg agccatccac catcaatgag aatcgacacg
      421 tcattcttcg agacaagatc gtcggacgca gtcgatatga ccctcgtgcc ggcattgagg
      481 atcttcacct catctccctg agccaacgag cccagcagcg atgcgacctc gttcgttccc
      541 gagtcataga tgtacgtcgc cgcgcggacg atcccaggga caatcgacag gcccacagcc
      601 aacatgacca ttcgtttttt catccttacc gcctttcata cagaaacaat aaccgaccaa
      661 cctcggcaca atagccaatc aacctcctca aacgctgatt agtgtaccat aattcacccg
      721 ctggcactta cacaaacttc atcgacagag tatcacgcca atcgcccaca tgaacacgaa
      781 aatcgctaat gagacgagaa aggttaaaag gacgccacgg ccgacatcgc ggccgtcggc
      841 acggacgagc gaacggaagc gggcgatcgt ctccggcttc tcgggacgcg tcaggaacgt
      901 cgccgcaagc cacaccaccg tcgtcacgcc gatcgtcagt aaaaggcgtt tccagaaaac
      961 gagcggtacc gcgaatgacg gccagacaag ctggaagaaa cacgcgcaga ggatcgacgc
     1021 ccccatcgcg gcgatttccg tccaggcgtt gatgcgcatc cagaaccacc gcaggagaaa
     1081 caccgcgccg gtgcccgcgc cgaccatcac catcaggtcg aatcctgcct tcgcgtccgc
     1141 aatgaacggc accagcagac agcccgcgag catcagccca accgaggtcc agcgcgcgac
     1201 gcggatcagt tcccgcccgt ccgcctgcgg acgcacgaac cgcacccaca catcgttggc
     1261 aacgtagttc gcgcccatcg agagatgcgc ggcgatcgtc gagaacaaag cccccatcaa
     1321 ggacgccgca acgagtccga gccagcccgc cggcacgaac ttgatcatcg ccggatacgc
     1381 catatcgttg ccgacgagtg caggatcgac atccggaaac gccgcgcgta tggacgcaag
     1441 gtcgggaaag acaaggatgg aggcgaaggc ggtgatatac cacggccacg ggcggagcgc
     1501 gtactgtacg atctgcgcga aaagcgtccc gccgagcgcg tggttcggcg tctttgccgt
     1561 caacatgcgt tggacgatgt aaccgccgcc gcccggctcc gaacccgaat accacacgtt
     1621 ccaccactga atcgcgaccg gcacgatgaa aaccgcaatg gcggtgtcca gatcatcgaa
     1681 tcgcggcagg aagtcgagct tcggacggat cgcggcgttc tgcatcagcg ccgcccatcc
     1741 gccgacctca ggttgcgata acgcgaatgc ggtcaccgcc accgcgccga tcatgatcaa
     1801 cgtgaaaaga tagaagtcgg cgtagatcgt tccgcggatg ccgccgatca tcgaataggc
     1861 gatgctggcg acagccgtaa ccgcgaggat cgtccagacg ggcacaccga aaagaacggc
     1921 ccccagcttc accgccccga gaagaacggt cgccatcacg atcacgttgt agatgacgcc
     1981 gagataaacg gcgcggaagg cgcggagaaa cgccgccggc ttccccgaat atcgcaattc
     2041 gtagaacgcg atgtccgtcg tcgctcccga ccggtgccag agcttcgcat agacgaagac
     2101 cgtgaggacg ccgcccgtga gaaacgacca ccaaacccag ttgccagcga gtccgttttc
     2161 ccgaattaac tgggtgaaca gattcgccga gttggtcgac gtcatcgcgg ccaccatcga
     2221 gatgccgatc aaccaccagg gcattgaacg ccccgactgg aaaaagtcgg aagacgtacg
     2281 tgcgcgacgc cggttggcga acgccggtat cgcgagcaac acgacaaaaa acgctacggt
     2341 caccaaccag tctgccagtg cgaatctcat gacccgttgc tactcctgtc tagcgttcac
     2401 ggattccggt gcgaagtcgt cgagttggaa cttccactcg ggacgtgtac ggaagggcgt
     2461 cttgaatgtt cttttcagga cccccttctt ctccaaatgg caataacatg cgacatcgca
     2521 ggcgcgtccg cagatcgagc aggtgtagga gtgctgcgcc ggcggataaa aatagatcgc
     2581 gtcaaggatc cgcttcgctt tctctgaatc gatctcggcc tcgcccgcga tgatcgcaag
     2641 ccggtcctcg aaatcaggga aggcctccgg cggcatgaac ggattcttcg tgccgttcgc
     2701 gccgttgtaa tagacggcgc aacgccacgg atcaatcgcg ccagactctg ggtcaataca
     2761 tttgcccgga caccccttca cacatgcgcc gcacctgtcg caaagatgcg gcgtcaccac
     2821 ctgatccggc tcaagttcag catccgtcaa gatgaagcac gtccgctgca tcggcccgaa
     2881 atcgtccgtc aggagcgcac cgtggaagcc gcgttcgccg agtccgcacc gcaccgccgc
     2941 gtccgtgaag tcgaactgcg gctcctcgac gcgtccgcgc gcaatcgcgt cataggcgac
     3001 ttccgggttc atgccatcct ccgcctccat gatcgtctgg tggcggcgtt ggggcacggc
     3061 cgtgaagccc ttggactcga tccgattcgc cagcctcaca agcgcgatcg gcatcaccgt
     3121 ctcctccatg ttctcgacgg acatcgtcgt atactggtag taggtcgttc cctcctcggt
     3181 accgcgatag ctaccgcgaa ggcaccggaa cgccaaaccg atgacactct tcacctgcgg
     3241 atagatcctg aaaatcttgt gatccgccgg gaaccgcgac gcaggcgcga cgccaaccag
     3301 atccgcgccg cattctttag ccaacttcag aagttcgtgc ttcacaactt cacccccttg
     3361 atgtgctccc agcaggcgac atcacacttg cgtccgcaca ggcacgcttg atacccccac
     3421 tgcgttcgcg gcaacgaata accgcgtgcc ttgttgtaat gctcccagca cttccacgag
     3481 tcgagtccct tctccacgtc gatgcatccg gcatcgcacg ccttcacgca ggcatcacag
     3541 cccgcgcaga gatcgtccgc gtacggcgcg ttcgtctcaa gcagagcgtc ggtgatgatg
     3601 aaggcatagc gcatgaacgg gccgtactcg cgattgatga cgcgtccgta cctgccgggc
     3661 atgccgatac cgcacgcccg agcggccttg ccgaagtcga tcatgacgtc cggcggcggc
     3721 ttgcccggct cgaccgcttc cgcatgggca agctcgtaga cgccgatcat ctcggggttc
     3781 gcggtcttgt cgcccttgac gcggaggttc ggcgtcgtcc gttgcaggca tgcgtcataa
     3841 ccggcgtctt caatgatcga agccatcttg aagagaaaaa tatcgaagta ttcctcatca
     3901 atcgctttca cgccgagtgt cgtatacgca tagaactgcg attcgtcctt catcgtcttg
     3961 tatagaccct tcggaacggg aatgccaaaa ccgatgatcg cctgcgcctt cgggcagacc
     4021 tgcttcggat cgtggcgcgg atcctctccc tcgaacagct tcagatctcc gacgccgaac
     4081 atagccgccc cgagttcacg cgccacctgc tcaatcgttt ctcgattcat cttccctcac
     4141 ctcaacccgt tacctgtcca ttttccacgc cttcggacgt gtacggagcg gattcttaaa
     4201 ccggccgtgt agacagccgc ccggtttctc gagcatctcg acacaagcgc ggatacagcc
     4261 gccgcacttg gccatgttgt agccgaccca gctcgggaag tactttgcca acgcccggta
     4321 gaccttcatg atctcttgtt cactcgcatc ggttttacct gcgaacagat caaggtcacc
     4381 gtccttgttt tcgtcccaga cctctttggg aacgaacgga ttgtagtaac gtccggcgtg
     4441 agtatagaag gcatagcact tccacatgtc gatatcgccc cactccatct tgtagtcgcc
     4501 gatcgaagcc gtacacttct tgccggattt aatctcagga atgcaattcc ccgggcactc
     4561 gcgcacacac gccccgcacc ggcggcagag cggcttgccg ttgtagatct cgtcatattc
     4621 gtcgaactcg gcgtccgtaa acagaaacgc gacgcgctgc aaggggccga actccggcgt
     4681 cagcagcatc ttcgaccacc cgatttcgcc gagaccgcag gcaaccgcgc agatgcggaa
     4741 ctggaactgc aagtccggcg ggacgttctc gcccggacgc gtcgcgcggg tgaagtcaac
     4801 ggaacggtga tgcgccgtcg aatccttcgc gttctcactc acctcgatct gttcctcagg
     4861 agagagcttc gcgtcctccg atccgtccat atcgctgaca catccacgcg cgccggtgtt
     4921 gcggtagaca aacgcctcgt agcccgcgtc ctcgatcgcc ttgccgacat ggtacagggt
     4981 cgccggcgca aggatctcgt tgatgccgcc gtaggccatc gacggatact ggtagaactg
     5041 cgtcccctcc tcaatgccgc gctgtacgcc gcgcgggata cgaaagacga gaccaaccat
     5101 cgatttgcag ttcgggaaca ggaacgtcgg attcatctcg tccggcgcat cgctcatgcg
     5161 cgaaatcggc gcgatgccgc aattatcggc gccggccttt ttggcaatct ccttgattcg
     5221 ttttgctgaa agcatgttac ctccaaagtg ccgaatgatc gggttcgagc agccccttga
     5281 tctctccgag atgcgatccg gtcgcatagg actccaggat acggacagga tgcgcgttga
     5341 tgcgcgtggc gagcttcatc gccgtgcaga gcccttcgta cttcgtcacg ctttccggtt
     5401 caatccagtt cgtccgttcc atccacttcg cgcggttctc cggtttggca agccactccc
     5461 agcacattcc ccggtacacg tacccattat ccttctgtgc gatctccgtt tcgtgcgcga
     5521 caagattatc gtggaacatg tgggccgagc agtggttgag gcatccggaa ttcgcaagga
     5581 tgaagagctc cttgccgtag gcatcgcacc acgtcttcgc cgcgcgcacg gcatcgagat
     5641 cgcggttgag ttcacgcttc aggtagtatc cgtcaaaaac gtcagcaaga tactccatac
     5701 cttcgatcgt tccgatctcc atgttcaccg atgcacgcgt cttcagctcc gggaaattat
     5761 ctttcacgaa cttcgcgata agcggagaag tcgtcgtcac gccctcaagc gatccatccg
     5821 cggcaaaacg ttccacggtt tcgccgatat gggaaaaaag cgacttcgca agcgactcgc
     5881 caccataaca ggtcgcgttg aaaaggaggt gcagcggcaa tcccgcttcc gccatccggc
     5941 cgaggtcgtc gagcagacgg tcctccgacc tgatcacggc acgccccgat gcccaaccgg
     6001 aagccgcgaa gtacacgtcg tgcatgacgt ccttacgcgc gataatcgct tcgatccacg
     6061 ccgcatcatc ctgatacccc acactgaatt tcatgtgtct atcttaccgc aattcatcga
     6121 cgttcattta cacaaatgcg cattatattt tgcactaaag cgaatatgcg a
//
LOCUS       DAOSRB010000168         6018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_12037_length_6018_cov_6.684890, whole genome shotgun sequence.
ACCESSION   DAOSRB010000168 DAOSRB010000000
VERSION     DAOSRB010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12037_length_6018_cov_6.684890"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLZ74_08745"
     CDS             complement(<1..262)
                     /locus_tag="PLZ74_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98451.1"
                     /translation="MDNGTLTKVLIAAYGAGVLTVVAQTATNNADTARAPEIEIEVTA
                     SPITQLEETTLDGANRVVVGRDQLDRLSATDLPTALRQVPGVS"
     gene            complement(725..2794)
                     /gene="dnaG"
                     /locus_tag="PLZ74_08750"
     CDS             complement(725..2794)
                     /gene="dnaG"
                     /locus_tag="PLZ74_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOR98452.1"
                     /translation="MAVRITESVVEEIRARVDIVDVIGSRVPLKKAGASFKACCPFHH
                     EKTPSFTVNPTRQSFYCFGCGEKGDVFSFVMKQDGLTFADAVKAMAERVGVVLQDRDD
                     QGSSGERAVLYAAHAAVAEYYRQCLLNLPEAEHARRYLAQRKLSDEIVKSFGIGYAPE
                     HAVEALLNALEKNRFPPEVLVRAGILCESDRGGPGRYFDRFHGRLMFPIWDRQGRVVA
                     FSGRLLTPRERVGKYVNSPETPIFTKSRVLYALHKASGKIVKHPRREAIVCEGQIDVI
                     RCHASGFETAVASQGTAFTRDHVKLLKRYADSVTLVFDGDAAGRKAALHTGTLFLEEE
                     IPVRVACLPVGEDPDSILRDQGPEPFRDLLERAVSLIAFQIETQMQDEPNPEAIDAVS
                     RVARGVIGTLATCPGGVLRTLLLQEAGERLHLPYSALEKDLEKQLEVLSRKAALAQTF
                     KTDGGAADPSGERRDEQCAGSGPSPEYNADPDIDPAFAPPSRLETLLCECLIEHERDP
                     AVFELVSRYLPLDLITHPFARDLVVVLLRQVQSGEDMLAGYYQQTIPEWIPLFDRLLA
                     HKQKMLCAREMTQEDAVQDLIRQLWIDRLKAEQQALGTESSPECDARRYTLSLRMKAL
                     RNRPWAQVCDLLAELGAPGATGSGIPAEAASAGAESCAHATDSASAVSDPEEFPPSAY
                     LPDEMPD"
     gene            3116..4996
                     /locus_tag="PLZ74_08755"
     CDS             3116..4996
                     /locus_tag="PLZ74_08755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOR98453.1"
                     /translation="MSTVYGRGGHPGRTRNAGWSRVKNDAIEQRGFRLLEQLDETSRT
                     QVWRAVQTNLDRTVILQILKEHAAQNPGETAHFLMIARRIARIKSEAVCALFDIVSEA
                     PLHYVVMEQVDGPTLEELVLQHGPLPFPQILRIAASLADGLDQMWRSAHLVHRNLKGA
                     TIRLDERGIAKITDFSLAIEAGPGIDATDMDQGLIVGTPCFLSPEQASGSRTLTTQSD
                     MYALGALLYYLATGHVPFEELDVVSILAAHCNGRIPPPHLLRNDLPKVFSWFLHRLMM
                     KSPENRYAGWDAVRQDLDRLRKGTSPASCVRPDDTAPSTVDAAPLREAVSDTPAAEQE
                     AAGPRIRMAGRPKNEKITAYQSKTIEEDHAREIRRETLVREGFCWLVLAVWLIGLFWV
                     RAVLEPAEGQPDARPAPEPPPIDETQAGDKTPEAEKGKASTDVPETSDVPVPPPSSAT
                     GTATDLPAVGEMHPALKTALSQALIKGNLIRARALVQSHPDPFRNQAELAAFLTDMPD
                     PDALVAEYLLDQTGKPLQWERNGKTRTVIPRAIVGSTAHLDANGNRIAVPLDTLSPEE
                     KLRWMNPARTAHQTAAQCLLLLHSPRRHEIRSKMAHCPLLAPFLDEALRLMPEGVTPP
                     AE"
     gene            complement(4977..5816)
                     /locus_tag="PLZ74_08760"
     CDS             complement(4977..5816)
                     /locus_tag="PLZ74_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR98454.1"
                     /translation="MRLVVNGIEIPEEVVTREAERLRHPYEEYVRANGGEPDEAQLRE
                     WAVEGVIESELVRQEACATQPIPSEARIRQELTQYAETYQPYPEAERAARARGGLQVR
                     RYLKQIRKDVPVPDEAAARNYFDTHAELFKVAEGVRLEHVCRFVDLASRPSVYMSLLQ
                     LKSDVEAGRVDWRGEAVPYYSDTYNRDGGAFAVVTRGELPEAIERDLFALQPGQWSDV
                     IDFGNGTLHLFKVTERLLPRALSFEEVRETVIQSLYRVAAEEAVYARIDELKNKASIQ
                     REA"
     gene            complement(5840..>6018)
                     /locus_tag="PLZ74_08765"
     CDS             complement(5840..>6018)
                     /locus_tag="PLZ74_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOR98455.1"
                     /translation="TPPVGTCLFIGCGVGKTTIAKVTVKALPFFAAMIIALMLITFVP
                     WISMAIPKASGLLK"
BASE COUNT         1197 a         1970 c         1739 g         1112 t
ORIGIN      
        1 tcgagacacc gggcacttgg cgcagggcgg tcggcaggtc ggtggcgctc aggcgatcga
       61 gttgatcacg tcccacgacc acgcggttcg cgccatcgag ggtggtctct tcaagctgcg
      121 tgatgggcga cgccgtcacc tcgatttcaa tctctggcgc acgcgccgtg tccgcattgt
      181 tggtcgccgt ctgggctacc acagtcaaaa cccccgcgcc gtatgctgcg atcaacactt
      241 tggtgagtgt gccgttatcc attgatccct cgcctcctct ttccatcggt cgtgttacaa
      301 tttcaagaaa cgtaacacaa tggcggcgac aggtcaagga cgggcgcaag acaaaatgaa
      361 ctgcgggcaa tatcacgcca ttcaggcact ggacgcgggg acggggaacg ggtattcttg
      421 tgacaaacgt gaggagaagt tatgatcccc gaccttcagg cgagtctgtt gtgcgacgat
      481 gtgcggcagg agcgtaacgg aaaattcatg ctgatcggca ttttcgacgg gctggtgcaa
      541 gccgtgaatc aaccggtctg cccgcgcatt tgcctggtga accgctggtg cgccggagcg
      601 ggcaccttca cccagcggtc acgcattgtg gcacctgacg ggttgacgct tgtgggagaa
      661 gggcaaccgg tgccgatcac gctctctaac gaccagcagg tcgcaaccac cgtggaggtc
      721 tttctcaatc tggcatttca tcaggcaggt acgcactggg tggaaattct tctggatcag
      781 aaactgcgga tgcgctatcc gttgcatgtg cgcaagattc agccccagcc gacgccgcct
      841 ccgccgggat ccccgaaccc gtagcgcccg gcgcgcccag ctccgccaac aggtcgcaca
      901 cctgcgccca cggccggttg cgtaacgctt tcatgcgcaa agacagggta taccgccggg
      961 cgtcgcattc cggcgaggat tccgtcccca gagcctgctg ctccgctttg agccgatcaa
     1021 tccacaattg ccggatcaag tcctgaaccg catcctcttg ggtcatctcg cgggcgcaaa
     1081 gcatcttctg cttgtgcgcg agcagacggt cgaacagcgg gatccactcc ggtatggttt
     1141 gctgataata tccggccagc atgtcctcgc cgctctgaac ctgacggagc agaaccacca
     1201 cgagatcacg cgcgaacgga tgggtgatca ggtcgagcgg caggtaccgc gagacaagct
     1261 cgaacaccgc cggatcccgc tcatgctcga tcaggcattc acagagcagc gtctccagac
     1321 gcgaaggcgg cgcaaaggcg ggatcgatat ccggatccgc attgtactcc ggagagggtc
     1381 ccgaacccgc gcactgttca tcacgccttt cgcccgaggg atcggccgcg cccccatcgg
     1441 tcttgaaagt ctgggccagc gccgctttcc ggctcaacac ctcgagctgt ttctcgagat
     1501 ccttctccag tgcggaatac ggcaggtgca gccgttcccc cgcctcttgc aacagcagcg
     1561 tgcgcaacac gccaccgggg catgtggcca gagtgccgat cacgccgcgc gcaacacggc
     1621 tgaccgcatc gatcgcctcg ggattcggct cgtcctgcat ctgcgtttca atctggaagg
     1681 cgataagcga aaccgcgcgt tccagcagat cccggaacgg ttcggggccc tgatcgcgca
     1741 agatcgaatc ggggtcctcc cccactggca gacaggccac gcggaccggg atctcctctt
     1801 ccagaaaaag cgttccggtg tgcagcgcgg ccttacgccc cgcagcatcg ccgtcaaaga
     1861 ccagggtcac gctgtcggca taccgtttga gcagtttcac atggtcgcgg gtgaacgccg
     1921 taccctgcga cgcgacagcg gtttcgaaac cggaggcgtg gcagcggatc acgtcgatct
     1981 ggccctcgca cacgatcgcc tcgcgccggg gatgtttgac gatttttccg ctggccttat
     2041 ggagcgcgta gagaacgcgg cttttggtga agatcggcgt ctccgggcta ttgacgtact
     2101 ttccgacccg ctcccttggc gtcaggagtc gtccgctgaa agccaccacc cgaccctgac
     2161 gatcccaaat cgggaacatc agccgcccgt ggaaacgatc gaaataacgg cccggaccgc
     2221 cgcggtccga ctcgcagaga atcccggccc gaaccaacac ttccggaggg aagcggtttt
     2281 tttcgagcgc gttcaagaga gcctcgacgg catgttccgg cgcgtatccg atcccgaagg
     2341 acttcacgat ctcgtcggac agtttacgct gtgccagata acggcgcgcg tgttccgcct
     2401 ccggcaggtt caacagacac tgccggtaat actccgccac ggcggcatgc gccgcgtaca
     2461 gaaccgcgcg ttcgcccgag gatccctgat cgtctctgtc ctgaagcaca accccgacgc
     2521 gctccgccat cgccttgacc gcatcggcaa aggtcagacc atcctgtttc atcacaaacg
     2581 agaacacatc gcccttttcc ccgcacccga aacagtagaa gctctgccgc gtggggttga
     2641 ccgtgaacga tggcgttttc tcatggtgaa aagggcaaca ggccttgaag gatgcccccg
     2701 cctttttcag cggcacacgg gatccgatca cgtcaacaat gtcgacccgg gcacggatct
     2761 cttctacaac ggactctgtg attctaactg ccatgaaccc ccttcaccag cctccccgtt
     2821 gccggcaccc gtttgttcgg gcggaaccgg cggggaacgg acgggatatg ggaagaacat
     2881 accatgccct tccctcgtct acaagccgga gcgataccac cccatctgtt tcagcctgtc
     2941 ttctgcggaa caggcccaga cgacgggaca ccttgatcca cagataacgc agattacaca
     3001 gaagaggatg atcgcaccac aaagggcgca aagtgcataa agactgattt gaccggagcg
     3061 gcctgtaacc tgtaacgatc ccggcgcatt gaccggacaa gccgcatctg gcacaatgag
     3121 caccgtatat ggacggggcg gacatcccgg ccgcacacga aacgccggat ggtcacgggt
     3181 gaagaacgac gccatagaac aacgcggttt ccgattgctt gaacaactgg atgagaccag
     3241 ccggacccag gtttggcgcg ccgtgcaaac caatctggac cggaccgtca tcctgcagat
     3301 cctgaaagag catgcggccc aaaaccccgg ggaaacggcg catttcctga tgatcgcccg
     3361 ccgtatcgcc cgcatcaaaa gcgaggcggt ctgtgccctt ttcgatattg tcagcgaagc
     3421 gccgttgcac tatgtggtca tggagcaggt cgacggaccc actctggaag aactcgtttt
     3481 acagcatggc ccccttccgt tcccgcagat tctccgaatc gccgcgtccc tcgctgacgg
     3541 tctggatcag atgtggcgtt ccgcgcatct cgtccaccgg aacctgaagg gtgcgacaat
     3601 ccgcctcgat gagcgcggca tcgcgaaaat cacggatttc agcctagcca ttgaggccgg
     3661 tcccggcatc gacgcaacag atatggacca gggcctgatt gtcgggacgc cctgttttct
     3721 ttcgccggag caagcctccg gatcccgtac gctgaccaca cagtccgaca tgtacgcgct
     3781 gggcgcgctg ctttattatc tggcgaccgg acatgtgccg ttcgaggagt tggatgtggt
     3841 gtcgattctc gcggcgcact gtaacggtcg gataccgcct ccgcatctct tacgcaacga
     3901 tctcccgaaa gttttctcct ggtttctgca ccggctgatg atgaaatcgc cggaaaaccg
     3961 ctacgccggg tgggatgcgg tccggcagga tctggaccgg cttcggaaag gcacttcccc
     4021 cgcatcctgc gttcgtccgg atgacacggc gcccagcaca gtggacgccg caccgttgcg
     4081 cgaagccgtt tccgacaccc ccgcagcgga gcaagaagcg gcgggtccgc gaatccggat
     4141 ggcgggccgg ccgaaaaatg aaaagatcac ggcgtatcag tcgaaaacca tcgaggagga
     4201 ccacgcgcgt gagatccgga gggaaaccct tgttcgggaa ggtttctgct ggctggttct
     4261 ggcggtctgg ttgatagggt tgttctgggt gcgcgctgtt ctggaacccg cggaagggca
     4321 acccgacgcg cggccggcac cggagccccc gccgatagac gaaacacagg ccggagacaa
     4381 aacacctgaa gcggaaaagg ggaaggcaag cacggacgtc cccgagactt ccgatgtccc
     4441 cgttccgccg ccgtcctccg ccaccggcac cgcgacggat ttgcctgcgg tcggggagat
     4501 gcatcccgcc ttgaagaccg cgctgtctca ggccctgatc aagggcaacc tcatccgcgc
     4561 ccgcgcgctg gtgcagtcac atcccgaccc cttccgcaat caggccgaac tggccgcgtt
     4621 tctcacggac atgccggacc ccgacgccct tgtcgccgaa tatctgctcg atcagaccgg
     4681 caaaccgcta cagtgggaac gtaacggtaa aacgcgcacg gtgatcccgc gcgccatcgt
     4741 cggcagcacg gcgcatctgg atgccaacgg aaaccggatt gcggtgccgc tggacacgct
     4801 gagtccggag gaaaaactgc gctggatgaa cccggcccgt actgcgcacc agaccgccgc
     4861 gcaatgcctg ttgctgctgc actctccgcg ccgccatgaa atccgttcga agatggcgca
     4921 ctgcccgctt ctggcgccgt ttctggacga ggcgttacgc ttgatgcccg aaggcgtcac
     4981 gcctcccgct gaatagacgc cttgttcttc agctcgtcga tccgcgcata caccgcctct
     5041 tccgcagcca cccgatacaa agactggatc acggtttccc gaacctcctc aaacgacagg
     5101 gcgcggggca acaggcgttc cgtcactttg aagagatgaa gcgttccgtt tccgaaatcg
     5161 atcacatcgc tccattgtcc gggttgcagc gcgaacagat cccgttcaat cgcctccggc
     5221 aactcgccac gcgtgaccac ggcgaacgcg ccgccgtcac ggttgtaggt gtcggaatag
     5281 tacggcaccg cctccccccg ccaatccacc ctccctgcct ccacgtcact cttgagttgc
     5341 agcagtgaca tgtaaaccga ggggcggctg gccagatcga caaaccggca cacatgttcc
     5401 agacgcaccc cttccgccac cttgaaaagt tcggcgtgcg tgtcgaaata gttccgggcg
     5461 gccgcctcat cgggcacggg cacatccttg cggatctgtt tcagataccg cctgacctgc
     5521 aaccccccgc gcgcgcgcgc cgcccgctcc gcttccggat acggctgata ggtttcggcg
     5581 tactgcgtca gctcttgacg gatgcgcgct tcggagggga tcggctgtgt cgcgcacgcc
     5641 tcctgccgca ccagctcgga ctcgatcacc ccttccaccg cccactcccg cagctgcgcc
     5701 tcatccggct cgccgccgtt cgcgcggaca tactcttcat acgggtgacg caaacgctcg
     5761 gcctcgcggg tcacgacctc ttcagggatc tcgattccgt ttacaaccag cctcattcgc
     5821 ggggtccttt cttcctgcgt tacttcaaca atccgctggc tttcgggatc gccatcgaga
     5881 tccacgggac aaaggtgatc agcatcaggg cgataatcat cgcggcaaaa aagggcaggg
     5941 cttttaccgt caccttggcg atggtcgtct tgcccacgcc gcagccgatg aagaggcagg
     6001 tgcccaccgg cggcgtgc
//
LOCUS       DAOSRB010000169         6003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_12063_length_6003_cov_7.978480, whole genome shotgun sequence.
ACCESSION   DAOSRB010000169 DAOSRB010000000
VERSION     DAOSRB010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12063_length_6003_cov_7.978480"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1081
                     /gene="nagB"
                     /locus_tag="PLZ74_08770"
     CDS             314..1081
                     /gene="nagB"
                     /locus_tag="PLZ74_08770"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOR98456.1"
                     /translation="MDITICPSAEEASRLAGEVINNAVRQKPDLVLGLATGSTPLLLY
                     KEMIAACSNGLDYSRVRTFNLDEYYGVPPDHPQSYRKFMNDNLFSHLNIAPENTHVPD
                     GKARDVTAHCAAYEAEIKAAGGVDIQVLGIGSDGHIGFNEPASSLASRTRRVTLTRQT
                     ISDNARFFASADEVPRYAISMGVGTILEARTCLLLCFGANKARAVRGAIEGGISQFNP
                     ASALQLHPDTRVYLDEEAAAELDLKEYYRWAAENRPQ"
     gene            complement(1083..1541)
                     /locus_tag="PLZ74_08775"
     CDS             complement(1083..1541)
                     /locus_tag="PLZ74_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOR98457.1"
                     /translation="MDEHAKIEVIARGVCVAGGQILLCHGRGSSLTYLPGGHVEFRET
                     ARQALEREIREELGCASEAGAFLGCCEHAFMQNGAPHAEVNLVFALRVPGLAPGAEVR
                     AAEAWIGFRWHALDDLAASGLEPAPLRTRIADWLRNPGGHVVSGEAWQDV"
     gene            complement(1583..2971)
                     /locus_tag="PLZ74_08780"
     CDS             complement(1583..2971)
                     /locus_tag="PLZ74_08780"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOR98458.1"
                     /translation="MGYTNGMISVLEELASRRRFGMRPGLETIQALLEDLGHPERETA
                     AIHIAGTNGKGAVAAMCEAVLRAAGYPVARYTSPHLVCLNERFLLNGKPVEDAALEAC
                     AREVGTAVRALESRTGTEVTFFECLTAIAFVLCRNAEIRLIALETGLGGRLDATNTVT
                     PLVSVITRIGRDHCDWLGDTLEQIAAEKAGIIKPGRPVVCAAMPEEARAVIAQSASRA
                     GSRFADAAETVEVTVTRNGLEGQTLKIATPNRSLPPVRLPLAGAFQVENVCAAVTALE
                     EVDACGVSLSDAAFVKGLEEVCWPGRFQLAVQTPPVIVDGAHNPEGARAIRQALKACR
                     IRPPLGLVAGFCGDKDALGHLRILAPVVKRAWAVPVPNPRTLTPEQTAGVMRMAGLGD
                     PQACAKLGAALDAAREWAAAEGGTVLVCGSLFLAGAALVDLGAYPWASPRHDPNDVLR
                     SPTPAEREQTRG"
     gene            3046..4545
                     /gene="tilS"
                     /locus_tag="PLZ74_08785"
     CDS             3046..4545
                     /gene="tilS"
                     /locus_tag="PLZ74_08785"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOR98459.1"
                     /translation="MSATVQHTPAPAVPEGLGQAVAEEVTRKRLLRGIRCLGLAVSGG
                     ADSVALFHLLRPLCEAAGIRTVVFHLNHGLRGEAADGDACFVRSLAHAAGIACITGRA
                     DLAGRTPDGQSPERVARDARLAFFRQAAEETGAEAIATGHQADDVAETLLLRLARGAG
                     ATGLAGLRPRTSLPRSTRILIRPLLSFPGAVLRAWLRDRGLDWREDHSNLDRTIPRNR
                     IRHALIPDLERFLQPGLRTRLCRSAAILREEDALLETLAGRALDALAPLQPEGLPDSL
                     PAVALLEHPLALQRRILRLWLFRQACPEAAGFAGVDALLDMCRDGKGRSVCLPGGKTF
                     RLHDGLLQLQPAREIPPPETSLSCPGVCRWGALEIVAEFSRGITGTVTGINRYPAVCT
                     LCADALAGRALLVRSRRPGDRIAPVGLNGTKKIQDLFTDEKIPEAVRDAIPLFECDGE
                     IAWVPGYRIGRRFAVPAADAASVRVTVRRAETDAVRGAPPRPDESDQPR"
     gene            complement(4533..5924)
                     /locus_tag="PLZ74_08790"
     CDS             complement(4533..5924)
                     /locus_tag="PLZ74_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR98460.1"
                     /translation="MSGQLTRRAMLSRLGLGGTAASMSLLAGRGFADADTDGDPSYPG
                     FAKNIRKVAPSMQDVPSARIEGGKVIQPARELPVFHTTDVVVVGGGSAGFAAAVAAAR
                     TGAKVALVERYGSLGGLFTNGMVLIVIGTGVKEGGAFKLVTRGICEEFLRRLEKMGPG
                     AITKLTDTVGQPTADPEAAKVLMDEMIQEAGVEMFFHAWGVDVIQNGNAVQGVVFESK
                     QGRQAILAKVVVDASGDGDVYFQAGAGYQQISHAMGFVYRVGNADRVDKTKLPKGTKP
                     PRLGSAEPIPSARWMNNLGPKGNGLDIRELSKIEMMHRKSAWNYVQKIRGTPGHEEVF
                     LMQTTPQIGVRATRLLDGVTSIDKKSAAAQTKFPDVVAVSGHDGLRLPEFQIPYGALL
                     PKTVDNVIAAGRCIACGADLIDRVRLIPVCIVTGHAAGVAAALAAQAGVRPRDVPAAD
                     IQKVLRGQGAYLG"
BASE COUNT         1037 a         2170 c         1762 g         1034 t
ORIGIN      
        1 ctgcggtgtc gggaacgcgg gcgtccctgt cgatcgtcgc gggtcgaatg tcgcgggtcg
       61 cgggtcgacg atcccgatac cgatagcgat accgatagcg atcccgatac cgatagcgat
      121 cccgaccccg atgaaacctg aaacccagaa acccagaaac cagaaaccca gaaacccaga
      181 aaccagaaac ccagaaacca aggagcattg aaaggttcaa ctgctctttt taggagttgc
      241 cgtatttgac acccgcgccg atttgaatta gtcttacatg tgattcatat cattacaaac
      301 gaagggagcg attatggaca ttaccatttg cccttccgcc gaagaggcca gccgcctcgc
      361 aggggaagtt atcaacaacg ccgtccgtca gaagcctgat ctcgttttag gcctcgccac
      421 cgggagcacg ccgctgttgc tctacaagga aatgattgcg gcctgcagca acggtctgga
      481 ttacagccgc gtgcgcactt tcaacctcga tgagtattac ggggtaccgc cggatcatcc
      541 gcaatcctat cggaaattca tgaacgacaa ccttttctcg cacctgaaca tcgcgccgga
      601 gaacacccac gtgccggatg gcaaggcccg cgacgtgacg gcccattgcg ccgcatatga
      661 ggcggagatc aaggctgcgg gcggtgtgga cattcaggtg ctggggattg gcagcgacgg
      721 ccacatcggc ttcaacgaac ccgcctcctc tctcgcctca cgcacgcgac gggtcaccct
      781 cacccggcag acgatcagcg acaacgcgcg ctttttcgct tccgccgacg aggtgccgcg
      841 ctacgccatc tccatgggcg tcggcacgat cctcgaagcg cggacgtgcc tgttgctctg
      901 tttcggcgcg aacaaggccc gggcggttcg gggcgcgatc gaaggcggga tctcgcaatt
      961 caaccccgct tcggccctgc aactccaccc cgacacgcgg gtgtatctgg acgaggaggc
     1021 cgcggcggaa ctcgatctga aagagtatta ccgctgggcc gccgaaaatc ggccgcagtg
     1081 agtcagacgt cctgccaggc ctcgccggag accacatgac cgcccggatt gcggagccag
     1141 tccgcaatcc gggtccggag cggtgcgggt tctaatccgg aggcggccag atcatcgagc
     1201 gcatgccacc gaaagccgat ccacgcttcc gctgcgcgga cttccgcgcc gggcgcaagg
     1261 cctggcacac gcaacgcgaa cacgagattg acctccgcgt gcggtgcgcc gttctgcatg
     1321 aaggcgtgtt cgcagcaacc gaggaacgcc ccggcctctg acgcgcaacc cagctcttcg
     1381 cggatctcac gctccaacgc ctgacgcgcc gtctcccgga attcgacgtg tccgcccggc
     1441 agataggtga gcgaggatcc ccggccgtga cagagcagga tctgtccccc ggccacacac
     1501 acgccccgtg cgatcacctc gatcttcgca tgctcgtcca taccttctcc tatggtttca
     1561 atctgcgtaa tctgcagaca ggctatcccc gcgtttgttc ccgttccgcg ggcgtcgggc
     1621 ttcgtaacac gtcattcgga tcgtgacgcg ggctggccca cgggtaggcc ccaaggtcca
     1681 ccaaagccgc gcccgcgaga aacagcgagc cgcacaccag cactgtgccg ccttccgctg
     1741 cggcccactc ccgcgccgca tccagcgccg caccgagttt cgcgcacgct tgggggtcgc
     1801 ccaacccagc catgcgcatc acccccgcag tctgctccgg cgtcagcgta cgcggattcg
     1861 gcaccggcac agcccaggcc cgcttgacca cgggcgccag gatccgcaga tgccccaagg
     1921 cgtctttgtc gccgcagaaa cccgccacca accccagcgg cggtcgtatc cggcacgctt
     1981 tgagcgcctg ccggatcgcc cgcgcaccct cgggattgtg cgcgccgtcc acgatcaccg
     2041 gcggtgtctg caccgccaac tggaaacggc cgggccagca gacctcttcg agtcccttga
     2101 caaacgccgc atcggacaga ctcacaccgc aggcatccac ctcttccaac gccgttacag
     2161 ccgcgcagac attctcgact tgaaacgcgc cggccagcgg cagacggacc ggcggcagcg
     2221 accggttcgg cgtggcgatt ttgagcgtct gcccctccag cccgttccgc gtcaccgtca
     2281 cctccaccgt ttccgcggca tccgcgaaac gcgagccggc ccgtgacgcg ctctgtgcga
     2341 tcaccgcgcg cgcctcttcc ggcatagccg cgcacacgac cgggcggccc ggcttgatga
     2401 tccccgcctt ctccgcggcg atttgctcca gcgtgtcccc gagccaatcg cagtgatcgc
     2461 gtccgatccg ggtgatcacc gacaccagcg gcgtaacggt gttcgtcgcg tccagccgcc
     2521 cgccgaggcc ggtctccagc gcgatcagcc gtatctccgc attccggcac aggacaaacg
     2581 cgatggcggt gaggcactcg aaaaaggtga cctctgttcc ggtccgggac tcgagcgcgc
     2641 gcaccgcagt gccgacctcc cgcgcacacg cctccagcgc ggcatcctcc accggtttcc
     2701 cgttgaggag aaaacgctcg ttcaggcaca ccagatgcgg cgaggtgtat cgcgccaccg
     2761 gatacccggc cgcccgcagc acggcttcgc acatcgccgc caccgcaccc ttgccgttgg
     2821 tgcccgcgat gtgtatcgca gccgtctcgc gttccggatg tcccaaatcc tccagcagtg
     2881 cctgaatcgt ctccagcccc ggccgcatac cgaaacgccg tcgggacgcc aactcttcca
     2941 acacagagat catgccgtta gtgtagccca tcccgacgcg ccccacaatt cccgattgcc
     3001 ggaatccggt tgtgccgcac tgcggttttc tcgtatgatg ggcacatgtc cgctaccgta
     3061 caacacactc ccgcgcccgc cgtccccgaa gggctcgggc aagccgtggc agaggaggtg
     3121 acgcgcaaaa gactcctgcg cggcatccgg tgcctcggtc tggcagtgtc gggcggcgcg
     3181 gactcggtcg cgctcttcca tctgctgcgt ccgctttgcg aagccgcagg gatccgtacc
     3241 gttgtgttcc acctgaacca cggcctgcgc ggcgaagccg ccgatgggga tgcgtgtttc
     3301 gtgcgctcgc tggcacacgc ggcgggtatc gcgtgcatca ccggccgcgc cgaccttgcg
     3361 ggccgcaccc cggacggcca atccccggaa agggtggcac gcgacgcgcg cctagccttc
     3421 ttccgacagg ctgcggagga aaccggggcg gaggccatcg ccacgggaca tcaggcggac
     3481 gatgtggccg agaccctcct gctgcgtctc gcgcgcggcg cgggcgccac cggtctcgcg
     3541 ggactccggc cgcgcaccag cctgccgcgc agcactcgga tcctgatccg cccgctcctt
     3601 tcctttcccg gcgcagtgct gcgcgcttgg ttgcgcgacc gcggtctcga ctggcgcgag
     3661 gatcattcta acctggatcg cacgatcccg cgcaaccgga tccgccacgc cctaatcccg
     3721 gatctggagc ggttcttgca gcccggtctg cgcacgcgac tttgccgttc cgccgcgatc
     3781 ctgcgcgaag aggacgccct gctcgaaacc ttggctggcc gcgcgctcga cgcgctcgca
     3841 ccgctccaac cggaaggcct cccggactcc ctgccggccg tcgcgttgct ggagcacccc
     3901 ttggcattgc agcggcggat tctgcgcttg tggctcttcc ggcaggcgtg tcctgaagct
     3961 gcggggttcg cgggtgtgga cgccttgctc gacatgtgcc gcgatgggaa aggccgatct
     4021 gtttgcctcc ccggcggaaa aacattccgc ttgcacgacg ggttgctcca actccaaccc
     4081 gcgcgcgaaa tccctccgcc ggagacctcc ctgtcctgtc cgggagtttg ccgctggggc
     4141 gcgttggaga tcgtggccga attttcgcgc ggcatcaccg gaaccgtcac cggcatcaac
     4201 cgttatccgg ccgtgtgcac gctgtgcgcg gacgcgctcg cgggacgcgc gttgctcgtg
     4261 cgcagccgaa gacccggaga ccgcatcgcg ccggtcggtc tgaacggcac aaaaaaaatc
     4321 caggacctct tcacggatga aaagatcccg gaagcggtgc gcgacgccat tccgcttttc
     4381 gaatgcgacg gggagattgc ctgggtgccc ggctaccgca ttggacggcg tttcgccgtg
     4441 cccgcagccg acgccgcgag tgtgcgcgtc acggtgcgtc gggcggaaac ggacgctgtg
     4501 cgcggcgcgc ccccccggcc cgacgagtcg gatcagccca gataagcgcc ctgcccgcgc
     4561 aggaccttct gaatgtcggc agccggcaca tcgcgcggac ggactccggc ttgggcggcc
     4621 agtgcggcgg ccacacccgc cgcatgcccg gtcacgatgc agaccggaat cagacgcacc
     4681 cggtcaatca ggtccgctcc gcaggcgatg cagcgtccgg ccgcgatcac gttgtccacc
     4741 gtcttgggca gcagcgcccc gtacgggatc tggaactccg gcagacgcag accgtcgtgt
     4801 cccgagaccg ccaccacatc ggggaacttc gtctgggcgg ccgcgctttt cttgtcgata
     4861 ctcgtcaccc cgtccaacaa ccgtgtcgcg cgcacaccga tctgcggcgt ggtctgcatc
     4921 agaaagacct cttcatggcc cggcgtgccc cgaatcttct gcacgtaatt ccaagcggat
     4981 ttgcggtgca tcatctcgat cttcgacaac tcgcggatat ctaaaccatt gcctttgggt
     5041 ccgaggttat tcatccaccg cgccgacggg atcggctcgg cactgccgag acgcggcggt
     5101 ttcgtgccct tcggcagctt ggtcttgtcg acacggtccg cattccccac gcggtagacg
     5161 aatcccatgg catgggagat ctgctgatat cccgcgcccg cctgaaaata gacgtcgccg
     5221 tcacccgagg catccaccac caccttggcg aggatcgcct gccgtccctg cttgctctcg
     5281 aacaccacgc cctgcaccgc attgccgttc tggatgacat ccacgcccca ggcgtggaag
     5341 aacatctcga cccccgcctc ctggatcatc tcatccatca gcactttggc ggcctcggga
     5401 tctgccgtgg gctggcctac cgtgtcggtc agcttggtga tcgcacccgg gcccatcttc
     5461 tccaagcggc gcagaaactc ttcgcagatg ccgcgtgtga cgagcttgaa cgcgccaccc
     5521 tccttcacgc cggttccgat cacgatcagc accatgccgt tggtgaacaa cccgcccaga
     5581 ctgccgtacc gttcgaccag cgccaccttg gcaccggtgc gggccgctgc caccgccgcc
     5641 gcgaacccgg ccgaaccgcc gccgaccacc accacgtccg ttgtgtggaa aaccggtagc
     5701 tcgcgcgccg gttggatcac cttgccgccc tcgatccggg cggaaggcac gtcctgcatc
     5761 gacggcgcga ctttacggat gtttttcgcg aaccccgggt aagagggatc gccgtccgtg
     5821 tccgcatccg caaaaccacg gccagccaga agcgacatgc ttgccgccgt cccccccaat
     5881 cccaacctgc tcaacatcgc tctacgcgtc agctgtccgc tcatcgttat ccctcccgga
     5941 tggtttatcc tcgtttcacc ataacgcttt tcttccgccg ctgtcaatcc gccgcgcgcc
     6001 ctt
//
LOCUS       DAOSRB010000170         5971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_12157_length_5971_cov_9.812711, whole genome shotgun sequence.
ACCESSION   DAOSRB010000170 DAOSRB010000000
VERSION     DAOSRB010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12157_length_5971_cov_9.812711"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PLZ74_08795"
     CDS             complement(<1..1174)
                     /locus_tag="PLZ74_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOR98461.1"
                     /translation="MFLILILLAVAVVALSGLPGILLRRSDDGGERISAISMVCAACV
                     GLIGALGGLLGYGGKPLALTWPAVGGGSVGLDALSAFFLVPVFLIGGLGAVYGLSYWP
                     HRRHPVSGRRLRFFWGMLVAGMALLVVARHAMIFLLGWEIMALSAFFLVTAEGHRIAS
                     RQAGWIYLISTHVSVLALFALFAIWRHVTGSFNMTPAAPQALSQGVTALCMVLVLVGF
                     GLKAGVMPLHFWLPGAHANAPSHVSAIMSGVVIKTGIYGMIRWSALLPDVPVAWGALV
                     LLLGALSGVLGVLFALGQHDFKRLLAYHSVENIGIILMGFGVALLGRAVQRPEWVTLG
                     FGACLLHVWNHGLFKPLLFFCAGAVMRVTGTRQMDQLGGLAKRMPWTAAAFLVGAVA"
     gene            complement(1256..1331)
                     /locus_tag="PLZ74_08800"
     tRNA            complement(1256..1331)
                     /locus_tag="PLZ74_08800"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1296..1298),aa:Ala,seq:cgc)
     gene            1548..2894
                     /locus_tag="PLZ74_08805"
     CDS             1548..2894
                     /locus_tag="PLZ74_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOR98462.1"
                     /translation="MVDGTVLKGYADQIRATGRIDPALYEKFNVKRGLRNADSTGVLV
                     GLTRIGNVHGYVISESEKVSVPGQLFYRGIDVNDLVEGFQSEKRFGFEECAYLLLFGE
                     LPDSRQLEVWQETLDAYRRLPNDFKENAIIRTPGKDIMNSIARAVLMGYIYDSDPDNL
                     DIENALRQSIELIAQFPTIAAYAYQAKAHYHLGQSLMVRYPEKGKSTAENFLMLIRED
                     SLYTQLEAELLDLCLVLHAEHGGGNNSAFTTHVVTSAFTDCYAAVAAATLSLKGTRHG
                     GANLRVMKQMREIKENVENWDREGEVADYLVKILNREAGDGSGLIYGLGHAVYTLSDP
                     RTEMLREKARALAKTKGRDEEMALYETIERIGPEIFNRNRAKPRDLCANVDFYSGFVY
                     DMLNIPIDLYTPIFAISRVVGWCAHRIEELLNGGPIIRPAYKAVFERRPYKPMAER"
     gene            2924..3067
                     /locus_tag="PLZ74_08810"
     CDS             2924..3067
                     /locus_tag="PLZ74_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98463.1"
                     /translation="MGILNYNPAMRYRPFGTRTGVLVSALGFGAMRLPQSEGHIDLPA
                     QSI"
     gene            complement(3271..4644)
                     /locus_tag="PLZ74_08815"
     CDS             complement(3271..4644)
                     /locus_tag="PLZ74_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOR98464.1"
                     /translation="MLARQDVLSTLRMVREEHLDVRAVTLGINLNVCANPDPERLACG
                     VYERIVAKAGPLVWFCDEVSDKYALPIVNKRIAVSPVSLLLEGHTPETAVRVCQALDA
                     AAEAVQIDLLGGFSALVQKGMTPGEQTLVEALPQALTATRRVCSSFNVGTTRTGIQVD
                     AVNRIARKIIETAQASRATRGFGCAKIVVFANMPEDNPFMAGALLGPGEPECVINVGV
                     SGPGVVKRAVDRLLQNHPAASLDEIAEEIKHTAFRVTRTGELIGREVAERLGIPFGVV
                     DLSLAPTPRIGDSVGEIYQSMGISQIGAPGTTAAVMLLNDAVKKGGAFASSSVGGLSG
                     AFIPVMEDHALSRAVTEGTLTLEKLEAMTAVCSVGLDMILLPGDTSAETVAGIILDEA
                     AIGIANRKTTGVRVIPVPGAACGEFVDFGGLFGSGVVTRPACPTGAAAFVRRGGRIPA
                     PIHSMMN"
     gene            complement(4661..5326)
                     /locus_tag="PLZ74_08820"
     CDS             complement(4661..5326)
                     /locus_tag="PLZ74_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98465.1"
                     /translation="MTTSRHPPLRRFVTSVLVPDRVGLLRDITQAVFALEGNIGGIRQ
                     TLVDGFFNLVFTSEHPAAVTPENLRALLDAALESEAVVTVLDRPETPLPVLADGARFV
                     VMTRGEDKPGTILAITSFLVARGINIEDWMVELEAGSVIYLAQVTVPESADFRQLQTA
                     FRQEMAERGLNALLCHENIFRATNEIGPIRALISNRVRDAGPSEQTPPVQNCNPKPET
                     CNV"
     gene            complement(5389..5464)
                     /locus_tag="PLZ74_08825"
     tRNA            complement(5389..5464)
                     /locus_tag="PLZ74_08825"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5429..5431),aa:Thr,seq:cgt)
     gene            5731..>5971
                     /locus_tag="PLZ74_08830"
     CDS             5731..>5971
                     /locus_tag="PLZ74_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98466.1"
                     /translation="MLMKPTLIIFFTFLTVCAALAAPDARRELMARVVACDEAADAAV
                     AAIRTPEELAAKQRAWRATWLESLGGLPERTPLEDR"
BASE COUNT         1265 a         1836 c         1833 g         1037 t
ORIGIN      
        1 tcgccacggc accaacgagg aaagcggcgg ccgtccacgg catccgtttg gcgaggccgc
       61 ccagttgatc catctggcgc gtgccggtca cccgcatcac cgcgccagcg cagaagaaga
      121 gcagcggctt gaacaagccg tgattccaca catgcagcag gcaggcgccg aaacccagcg
      181 tgacccactc cggacgctgt acggcacgcc ccagcaacgc gacgccgaaa cccatcagga
      241 tgatgccgat gttctcgaca ctgtgatagg ccaacaggcg tttgaagtcg tgttgcccga
      301 gggcgaacaa cacgccgagc acgccgctca gtgcgcccag cagcaagacc agcgcgcccc
      361 atgcgaccgg cacatccggc aacaacgcgc tccaccggat catcccgtag atcccggttt
      421 tgataacgac gcccgacatg atcgccgaaa catggctggg ggcgttggcg tgtgcgccgg
      481 gcaaccaaaa atgcaacggc atcacaccgg ccttgagacc gaaccccacc agaaccagca
      541 ccatgcaaag ggccgtcacc ccttggctca acgcctgcgg tgccgctggc gtcatgttga
      601 atgaaccggt aacatgccgc catatcgcga acagcgcaaa cagcgccagc acactcacat
      661 gggttgaaat caaatagatc cagcccgcct gccgactcgc gatgcgatgc ccttcggcgg
      721 tgaccaaaaa gaaagccgag agcgccatga tctcccagcc cagcaggaag atcatcgcat
      781 gacgcgcgac caccagcagc gccatgcccg cgaccaacat tccccagaag aaccgcagac
      841 gacgcccgct caccggatgg cggcggtgcg gccagtaact gagcccatag acggcaccca
      901 agcccccgat caaaaaaacc ggaaccagaa agaacgcgct cagcgcgtcg agtcccacgc
      961 ttccgccgcc gaccgccggc cacgtcagcg caagcggctt gccgccgtac cccaacagac
     1021 cgcccaaagc gccgatcaga cccacgcacg cggcacacac catagagatg gcggatatcc
     1081 gttcaccgcc gtcgtcagac cgccgcaaaa gaataccggg caggccgctc aaggcaacga
     1141 cggcaacggc cagcaaaata aggatgagaa acaccagcgc tcctttcgca cgcgaccgca
     1201 gtaggtctcg ccacacaaaa aaagaccgcc gagcggcggc ctctcttttc aatcatggtg
     1261 gagcgaagga gattcgaact cctgaccccc acgttgcgaa cgtgatgctc taccaactga
     1321 gctaccgccc catgaatccc aaaaagtgca gtacatgatg ccaaagaggg ggccgttcgg
     1381 caagcgtgat ttttcgcgat tcatccgcga gaaagagttg acccctccta gggaacccgc
     1441 tacactcacg gctccttaaa agcgggtagt gtttgttccg ataggtaaac acgggtattt
     1501 ttccgcaaaa ggaggcaggg atgaagacag agcaatccga acatctgatg gtggatggga
     1561 cggttctgaa gggctatgcg gatcagatcc gcgccacggg acgaatcgat ccggcactat
     1621 acgagaagtt caatgtcaag cgcggattgc gtaacgcgga tagtacgggt gtgctggtgg
     1681 gactgacccg catcgggaat gtgcacggct atgtgatctc cgagagcgag aaggtctccg
     1741 tgccgggcca actcttttac cggggcatcg acgtcaatga tctcgtggaa ggcttccaga
     1801 gcgagaagcg tttcgggttt gaggagtgtg cctatctgct gcttttcggc gagttgccgg
     1861 actcgcggca gttggaagtg tggcaggaga cgctggacgc gtaccgtcgg ttgccgaatg
     1921 acttcaaaga gaacgcgatc attcgcacac cggggaaaga catcatgaac agcatcgcgc
     1981 gtgcggtgct gatgggctat atctacgatt cggatcccga caatctggac atcgagaatg
     2041 cgctgcggca gagtattgaa ctcatcgccc agttcccgac catcgccgcg tacgcttatc
     2101 aggccaaggc tcactaccac ctggggcaga gcctgatggt gcggtatccg gagaagggca
     2161 agagtacggc cgagaatttc ctgatgctga ttcgcgagga cagcctctac acgcagttgg
     2221 aggcggagct gctggacctg tgtctggtgc tgcacgcgga gcacggcggc ggcaacaact
     2281 cggccttcac cacgcatgtg gtgacctcgg cgttcaccga ctgctatgcg gcggtcgctg
     2341 cggccacgct ctcgctcaaa gggacgcgtc acggcggtgc caaccttcgc gtgatgaaac
     2401 agatgcgcga gatcaaggag aatgtggaga actgggaccg ggagggcgag gttgccgatt
     2461 acctcgtcaa gatcctgaat cgggaggcgg gtgacggtag tggactgatc tacgggttgg
     2521 gccacgcggt ttacacgctc tccgatccgc ggaccgaaat gctgcgcgag aaggcgcgcg
     2581 cgctggccaa gaccaaaggg cgcgatgagg agatggcgct gtacgagacc atcgagcgca
     2641 tcggtcccga gatcttcaac cgtaaccgcg ccaaaccgcg tgatctatgt gccaacgtgg
     2701 atttctactc gggattcgtc tacgacatgc tcaacatccc gattgatctc tacacgccga
     2761 tcttcgccat ttcgcgcgtg gtcggctggt gcgcccaccg gattgaggag ctgctcaacg
     2821 gcgggcccat catccggccg gcgtacaagg cggtttttga gcggcggccc tacaaaccga
     2881 tggcggagcg ctgacgggcg cctgccgcgt cccaacggcg gacatgggga ttctcaatta
     2941 caatccggcg atgcgatacc ggcccttcgg aacccggacc ggggtgctgg tttccgcgct
     3001 cgggttcggc gcgatgcgcc tgccgcaaag cgaagggcac attgacctgc ccgcgcaatc
     3061 gatttgatcg aaaccggctt ggacggaggc atcaactaca tcgacaccgc ctatgcctaa
     3121 tctacaacag gcacctgctg gtcgaccgca agaacacgtt gcaaccagcc ctctaccgta
     3181 gttttctgcc gaacgaaaag gcttcgggct gcatccggtg cggcacctgc gcaaagcgct
     3241 gtccgcagtc gatcgacatc cccgtccgtt tcagttcatc atgctgtgga tcggcgccgg
     3301 gattcgtcca ccgcgccgca cgaaggccgc ggcacccgtg gggcaggccg ggcgcgtcac
     3361 cacgccggac ccgaacaggc cgccgaaatc cacaaactcg ccgcatgccg cgccgggtac
     3421 cgggatgacg cgcaccccgg tggttttgcg gttggcgatt ccgatagcgg cctcgtcgag
     3481 aatgatcccc gcaacggttt cggcggaagt gtcgccgggc agcaggatca tgtccagccc
     3541 cacggaacag accgcagtca tggcctcgag tttctccagc gtcagggtgc cctcggtgac
     3601 ggcccgggag agggcgtgat cctccatcac cggaatgaag gcgccggaaa gtccgccgac
     3661 cgagctggaa gcgaaggccc cgcccttttt gacggcgtcg ttgagcagca tcaccgccgc
     3721 agtggtaccc ggcgcgccga tctgactgat tcccatgctt tggtagatct cgcccaccga
     3781 atcaccgatc cgcggtgtgg gtgcgagcga aagatccacc acgccgaacg ggataccgag
     3841 gcgttcggcc acttcgcggc cgatcagttc gccggtgcgc gtcacgcgga aggcggtgtg
     3901 tttgatctcc tcggcgatct catcaagcga tgccgccgga tgattctgca acagccggtc
     3961 cacggcgcgt ttgaccacgc cgggtccact gacaccgaca ttgatgacgc actccggttc
     4021 accggggccg agcagcgcgc cggccatgaa ggggttgtcc tcaggcatgt tggcgaagac
     4081 cacgatcttg gcgcagccga agccgcgcgt tgcgcggctg gcttgtgcgg tctcgatgat
     4141 tttgcgcgcg atccggttca cggcatccac ctgaatacct gtccgggtgg tgcccacgtt
     4201 aaatgaggag cagacacgac gggttgcggt cagagcctgc ggcagggctt cgaccagggt
     4261 ctgttcgccg ggggtcatcc ccttttgcac cagggctgaa aagccgccga gcaaatcgat
     4321 ctgcaccgct tcggctgccg catcgagcgc ctggcagacc cgtacggcag tttccggcgt
     4381 gtggccttcg agcagcaggc tcaccggcga gaccgcgatg cgtttgttga cgattggcag
     4441 cgcgtacttg tcgcttacct catcgcagaa ccaaaccagc ggaccggcct tcgcgacgat
     4501 tcgttcatac accccgcagg cgaggcgttc cggatcgggg ttggcgcata cgttgaggtt
     4561 gatgccgagg gtcacggccc gcacatcgag atgttcctcg cgcaccatgc ggagggtgga
     4621 gagcacgtct tgtctggcta gcatagggat atccggcagg ttacacgtta caggtttcag
     4681 gtttcggatt gcagttctgc acggggggtg tttgttctga cggcccggca tcgcgcaccc
     4741 tgttgctgat cagcgcccgg atcgggccga tctcgttggt ggcgcggaag atgttttcgt
     4801 ggcagagcag cgcgttcagg ccgcgctcgg ccatctcctg ccggaacgcg gtttgaagct
     4861 ggcggaagtc tgcggactcg ggcaccgtca cttgggcgag gtagatcacg ctgccggcct
     4921 ccagttccac catccagtct tcaatgttga tcccgcgcgc gaccagaaac gaggtgatgg
     4981 ccagaatcgt tcccggtttg tcctctccac gcgtcatcac cacaaagcgg gcaccatccg
     5041 cgagaaccgg taacggggtt tccgggcggt cgagcacggt cacaaccgct tcggactcca
     5101 gcgcggcgtc caacagggcg cgcaggttct caggtgtcac agcggcggga tgttcggagg
     5161 tgaagaccag attgaaaaag ccgtcgacca atgtctggcg gatccctccg atgttgcctt
     5221 cgagcgcgaa cacggcctgg gtaatgtccc gcaacaaccc gacgcggtcg ggcacgagca
     5281 ccgatgtgac gaaccgccgc agcggcggat ggcgcgatgt ggtcatgata accctgtgaa
     5341 aaaggcgaaa ccccgaaaat cgcggatacc gggtttcggg gtttcaaatg gagccaacga
     5401 tccggatcga acggacgacc tgatcattac gaatgatctg ctctaccagc tgagctacgt
     5461 tggcttgaaa aacggatgta ctataccgat tcgtccgcag agaagtcaag cgcgctgtcc
     5521 gcgcagccaa aaatccgcag tgtaatctgg aaatccttgc aaaacgctgc gccagcatgt
     5581 aaaaaagagc cgtaatcatc atcgggtcgt aacgcgcaga catggaaaaa ctgcaatgaa
     5641 aacaatttca gtccattgtg tcagcaccgc cgtgttttat ttggcggaat tctgcgtggc
     5701 gcggagacgg agtccgaatg tgccagtggg atgctaatga aacctactct aatcatcttc
     5761 tttacctttt taaccgtctg cgcggctctt gccgcgccgg acgcacggcg cgaactaatg
     5821 gcgcgcgttg tcgcctgtga cgaggcggcg gacgccgctg tagcggcgat tcgcacgccg
     5881 gaggaactgg ctgcgaagca gcgggcgtgg cgtgcgacgt ggctggagag cctcggcggc
     5941 ctgccggagc gaacgccgct cgaagaccgc a
//
LOCUS       DAOSRB010000171         5913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_12311_length_5913_cov_6.974565, whole genome shotgun sequence.
ACCESSION   DAOSRB010000171 DAOSRB010000000
VERSION     DAOSRB010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12311_length_5913_cov_6.974565"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLZ74_08835"
     CDS             <1..247
                     /locus_tag="PLZ74_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease"
                     /protein_id="HOR98467.1"
                     /translation="VVWMASGNAAQIPKPWYNLLQPVRTLTATIAGDMGEADQMSGSA
                     HYHVLFVMGLCLLLFSFGCNIAGEWVVARQRKKLRGE"
     gene            323..1600
                     /gene="pstA"
                     /locus_tag="PLZ74_08840"
     CDS             323..1600
                     /gene="pstA"
                     /locus_tag="PLZ74_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOR98468.1"
                     /translation="MASFLFIVLVPILWRGLGAYVFQGTVEHRRMLMGEFNRGSEESL
                     AAELKEVRQARAPVYQALEAFEKELAEMPASRRKELRPVFQSVRDGVASLLGSMPGEP
                     LPALPRRIYGKTRWDQARHGARLILFEEVWEHDDDGSHRRLEPRKALFAGTALEPLFD
                     LLEEKNLRRLMRPRLTLYARFLTDVSVDAHFFGGIGPEVMGTFYLALGAMLIAVPLGL
                     IAAIYFQEYAKGGPTMAFLRSCVNTLAGVPSIVFGLFGLAFFINTIKVSPGKSVLAGA
                     LTLSLLVLPTVIRAAEEAIAAVPQSYKEGALALGAGKWHTIMTVILPAALPGILTGIV
                     ISMGRAAGETAPIIFTAAVSVGKMVKASELFVMPTPALSWNLYNLCTEHEAVDEIRHV
                     QAGMAATLVLIVLALNLAAILLRARVAKRLKGG"
     gene            1683..2435
                     /gene="pstB"
                     /locus_tag="PLZ74_08845"
                     /pseudo
     CDS             1683..2435
                     /gene="pstB"
                     /locus_tag="PLZ74_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
     gene            2456..3163
                     /gene="phoU"
                     /locus_tag="PLZ74_08850"
     CDS             2456..3163
                     /gene="phoU"
                     /locus_tag="PLZ74_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOR98469.1"
                     /translation="MNPIFMREQEGLKEKILLLAGDVEMRLTEVLKATEQRDKDALYR
                     WIDRDSEIDGKEVQIEEECLKILALHQPVAKDLRFVVAVLKINNDLERIGDIVVNIAQ
                     KGIPLADTPVGDIFSSVIQMGRVSREMVKDALDALVNLDLEKANAVIRRDDDLDTMRS
                     NVWETVAQRATATNVGSITESLLLLNGVARDLERIGDHATNIAEDVAYLVSGKIVRHL
                     RRDVKEDKHGERKHSDS"
     gene            3135..3830
                     /locus_tag="PLZ74_08855"
     CDS             3135..3830
                     /locus_tag="PLZ74_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR98470.1"
                     /translation="MANESILIVEDEADIRELLRYHLEREGFQVSECGSAEDAKKFLA
                     RRLPDLVILDLMLPGTDGYAFCRMLRADERTAEIPVIMVTARDEEADIVAGLEVGADD
                     YLTKPFSARVLMARVRAVLRRAAAEPEDGEGVLSHGAIEIDRTYHTVKVDGKPVALTL
                     SEFKILNLFMKRPGAVFSRYQIVDAVHGQDYPVTDRSVDVQIVGLRRKLGKYGGLIET
                     VRGVGYRMKLERP"
     gene            3827..5689
                     /locus_tag="PLZ74_08860"
     CDS             3827..5689
                     /locus_tag="PLZ74_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR98471.1"
                     /translation="MRKRTMFWSFFGAHVVLLLLAVGAVSFYNWHISRYAFMRQWVRE
                     LEMQAKTAAALLPDDGGEVDEQYAAQFFERLRHHGIHRFTLILPNGRVLGDSDVDVEN
                     MESHEDRPEVIEAFEKGLGMRQRYSASLGKQMLYVAYRLPQEGAARAVIRVAVPVRFL
                     TSEMRAADKLLLSFFLGVLCVTLALSYVAALRITRPVSSLQLGLARIGSGNLSYRLPV
                     PRVPHLANLARSINRNAERLQRQVQALHEERNLRAMILENMTRGVIAIDRDRCVLDIN
                     AAARALLDIGDEQKVIAKPLGERIRNPEVFALIEACEQQNQMCEGEMTIDGKVTRRLQ
                     LRVSMLNDLGGERIGSLLVFSDVTLLRRLETVRQDFVSNVSHELRTPVTSIKGFAETL
                     LDGAVKDPEKAERFLKILVRQAGQLETTIRDLLELSRLEGHSPKALDCQRVPVGELLK
                     NAAELCQERADKRSVTLSLSCDEGLTVMVHAGLMEQALVNLIDNAITYGTDGTQKAQV
                     DIEAVSDGSAVSISVRDYGPGIESKHLDRLFERLYRVDKGRSRELGGTGLGLAIVKHI
                     ALIHNGSVDVESKVGKGSVFTIRLPLQCPDHETQEIKIDREPGAQVSSPAGIVR"
BASE COUNT         1183 a         1400 c         2026 g         1304 t
ORIGIN      
        1 ggtggtgtgg atggcgtccg gcaacgcggc ccagataccg aagccgtggt ataacctgct
       61 ccagcctgtc cgaacgttga ccgccaccat tgccggagac atgggggagg cggatcagat
      121 gtccggttcc gcccactatc atgtgctgtt cgtcatgggg ttgtgcctgt tgcttttcag
      181 tttcggctgc aacattgccg gtgaatgggt ggtcgcccgt cagcggaaaa aacttcgggg
      241 agagtaagac aggatgcgcc gttcctttag aacctttaca gaccgggcct tcacagcatg
      301 cgcgcttctc gcaatggcgg tgatggcgtc gtttctgttt atcgtgctgg tgccgatctt
      361 atggcgcggc ctcggggcct atgtgtttca aggcacggtg gagcaccgcc gcatgctgat
      421 gggcgaattc aaccggggat cggaggaaag ccttgccgca gaactcaagg aggtgcggca
      481 ggcgcgcgca ccggtttacc aggctctgga ggcatttgag aaagaacttg ccgagatgcc
      541 tgcatcgcgg cgtaaagagt tgcgcccggt gtttcagagt gtgcgggacg gggtcgccag
      601 cctcttgggt tcgatgccgg gcgagccgtt gcccgctttg ccgcgcagga tttacggcaa
      661 gacaaggtgg gatcaggccc ggcatggcgc gcgtctgatt ttgtttgagg aagtatggga
      721 gcatgatgat gacggcagcc atcggcgctt agaaccccgt aaagcgttgt tcgcgggaac
      781 ggctttggaa ccgctgtttg atctgcttga ggagaagaac ctgaggcggc tgatgcggcc
      841 gcggttgacc ttgtacgcgc gttttttgac ggatgtctcg gtggacgcgc atttcttcgg
      901 cgggatcggg ccggaggtca tgggcacctt ttatctggcc ttgggtgcca tgttgatcgc
      961 cgtgccgttg ggcttgattg cggctatcta ttttcaggag tacgccaaag ggggtccgac
     1021 gatggcgttt ctgcgttcct gcgtcaacac gctggccggc gtgcccagta tcgtgttcgg
     1081 cctgttcgga ctcgcgtttt ttatcaacac catcaaagtc tcacccggca agagtgtttt
     1141 ggcgggtgcg ctgaccctct ccttgctggt gctgccgacg gtgatccgtg ccgctgaaga
     1201 agcgatcgcg gctgtgccgc agtcgtacaa agagggcgcc ttggccttgg gcgcaggcaa
     1261 atggcacacg atcatgacgg tcattctgcc agcggcgctg ccgggcattt taaccgggat
     1321 cgtgatcagc atgggacggg cggcagggga gaccgcgccg atcattttca ccgccgctgt
     1381 gagtgtcggc aagatggtga aggcctctga gcttttcgtt atgcccacac ccgcgctctc
     1441 ttggaacctg tacaatctct gtaccgagca cgaggccgtg gatgaaatcc ggcatgtgca
     1501 ggccggcatg gcggccacgc tggtgctgat tgtgctcgcg ttgaatctgg ccgcgatcct
     1561 gttgcgggcg cgggtggcga aaaggctgaa gggcgggtga gcacgataac gtgcggaagt
     1621 gctgaaggct tgaaggatga gagtgtgaga gtggtaaaat gaatgaaaat gcgacaaagg
     1681 tgaagacgca tgtggaagct cgcggttttt ccgttttcta cggttcgcac gaggcggtcc
     1741 ggaaggtgga cttggctgtg ccgtgcgggc tggtgacagc gattatcggt cctagcgggt
     1801 gcggcaagag cacgtttttg cgggcgatca atcgcatgaa cgatctgata ccgggttgcc
     1861 atactgcggg cgatctatat tttgacgggc agaatgtgta cgggagtgat gtggacacgg
     1921 cgattctccg gcgcaggatc ggtatggtct ttcagaagcc caacccgttc ccgaaaagca
     1981 tttacgataa tgtcgcgtac ggtccacggt tacacgggag gcacaccaaa gcggagctgg
     2041 atgtgattgt cgaggagagt ctggttggtg cggcgttgtg ggacgaggtc aaagaccgct
     2101 tgtacaagaa tgcgttgggg ttgtcgggtg gccaggcgca gcggctctgt ctggcgcggg
     2161 cgttggccgt caagcccgag atgttgctga tggatgagcc gacgtccgcc ttggatccga
     2221 aggccacggc gcggattgag gaactgatcg tccagttgcg gggtaactac acgatcgcga
     2281 tcgtcacgca taacatgcaa caggctgccc gggtctcgga taaaaccgct tttttttatg
     2341 agggcgatct tgtagaattc gctccaacca aggagctgtt cacgaggccc cgagagaagc
     2401 atacggaaga ctacatcacc ggaaggttcg gttgaggaga acgggagggt cgcaaatgaa
     2461 tcctattttc atgcgggagc aggaaggctt gaaggagaag attttgcttt tggcaggcga
     2521 tgtggagatg cgtctgaccg aggtgctgaa agcgacggag cagcgtgaca aggacgcgtt
     2581 atatcgctgg atagaccggg attcggagat cgacgggaag gaggtccaga tcgaggagga
     2641 gtgtttgaaa attctggccc tgcaccagcc ggtcgccaag gatctgcgtt ttgtggtcgc
     2701 ggttttgaag atcaacaacg acttggaacg catcggggat attgtggtca atatcgcgca
     2761 aaagggaatt ccgcttgcgg ataccccggt gggcgacatc ttttcttcgg tgatccagat
     2821 ggggcgggtt tcccgtgaga tggtgaagga cgctttggac gcactcgtga acttggattt
     2881 ggagaaggcg aacgcagtga tccggcgaga tgatgatctg gacacgatgc ggagcaacgt
     2941 ctgggaaacc gtggcgcaac gggccacggc aacaaacgtc ggcagcataa ccgaatcgct
     3001 gctgctgctg aacggggtcg cgcgcgatct cgaacggatt ggcgatcatg ccacaaacat
     3061 cgcggaggat gtcgcgtatc ttgtgagcgg gaaaatcgtg cgtcatttgc gaagagacgt
     3121 gaaagaggat aagcatggcg aacgaaagca ttctgatagt tgaagacgag gcggatatcc
     3181 gtgagctgtt gcgctatcac cttgagcgtg aaggctttca ggtctccgaa tgcgggtcgg
     3241 cagaggatgc caagaagttt ttggcccgca ggttgccgga tctggtgatc ctcgatctga
     3301 tgttgccggg cacggacgga tacgctttct gccggatgtt gcgcgcggat gagcgcaccg
     3361 ccgagattcc ggtgatcatg gtcacggcgc gggacgagga agccgatatc gtggccgggc
     3421 ttgaggtggg ggcggatgat tatctgacca agccgttcag cgcgcgggtt ctgatggccc
     3481 gcgtgcgggc ggttttacgc cgtgcagcgg cagagcctga ggacggggag ggggtgctga
     3541 gccatggcgc catcgagatt gaccgcacgt atcatacggt taaggttgac ggcaagccgg
     3601 tggcgctgac attgagcgag ttcaaaatat tgaatctctt catgaaacgg cccggcgcgg
     3661 tgttctcgcg ataccagatt gtggacgcgg tgcacggtca ggattatcct gtgaccgacc
     3721 ggtcggtgga tgtccagatt gtggggttgc gccgtaagtt agggaaatat ggcggattga
     3781 ttgaaacggt gcgcggggtg gggtaccgga tgaaattgga gcggccgtga gaaaaagaac
     3841 gatgttctgg agctttttcg gcgcgcatgt cgtgctgctg ctgcttgcgg tcggtgcggt
     3901 ctccttctat aactggcata tcagcaggta cgcttttatg cggcagtggg ttcgcgaact
     3961 ggagatgcag gccaagacgg cggcggccct gttgccggat gacggcggag aggtcgacga
     4021 acagtacgcg gcccagtttt tcgagcggtt gcgtcatcac ggcatacacc gtttcacgct
     4081 gattctgccg aacggacgtg ttttggggga ctcagatgtt gatgtcgaaa acatggagtc
     4141 gcatgaagac cgccctgaag tgatcgaggc atttgagaaa ggtctgggta tgcggcagcg
     4201 gtacagcgcg agcctgggga agcagatgct ctatgtggcg tatcggcttc cgcaagaggg
     4261 ggccgcgcgc gcggtgatcc gtgtggcagt gccggtgcgc ttccttactt cggagatgcg
     4321 cgctgctgac aaactgctgt tgtcattttt tctgggcgtc ttatgtgtga cattggcgct
     4381 gagttatgtg gctgccctgc ggatcacgcg tccggtatca tcgcttcagt tgggattggc
     4441 gcgaatcggc agtgggaact tgtcgtaccg gttgccggtc ccgagggttc cccatctggc
     4501 gaatttggcg cggtccatca accggaatgc ggagcgcctg caacggcagg ttcaggcact
     4561 gcatgaggag cgtaatctgc gggcgatgat tctggagaac atgacacggg gtgtcatcgc
     4621 gattgatcgt gaccgctgcg ttcttgacat aaacgcggcg gcccgtgcgc tgttggatat
     4681 cggggacgag caaaaggtga ttgccaaacc gctcggcgaa cggattcgga atcctgaagt
     4741 tttcgcgttg atcgaagcgt gtgaacagca aaaccagatg tgcgaaggcg agatgacgat
     4801 cgacggcaag gtgaccagac ggctgcagtt gcgggtgtcg atgttgaacg acttaggcgg
     4861 ggaacggatc ggctccttgc tggtgttcag cgatgtcacg ttgttgcggc gattggaaac
     4921 cgtgcggcag gattttgtgt cgaacgtttc gcatgagttg cgcacgccgg tgacatcaat
     4981 caagggcttt gccgagaccc ttctggatgg ggcggttaaa gaccctgaaa aggcggaacg
     5041 cttcctcaaa atccttgtga ggcaggcggg gcaactggag acgaccatcc gtgacttgct
     5101 ggagctgtcc cgattggagg ggcattcgcc taaagcgctc gactgtcagc gggtgccggt
     5161 cggggaactc ttgaagaatg cggcggaact ctgtcaggag cgtgctgaca agcggagcgt
     5221 cacgctttcg ctctcgtgtg atgaggggct gaccgttatg gttcatgccg ggttgatgga
     5281 acaggcgctt gtgaacctga tcgacaatgc catcacatac ggtacggacg ggacacagaa
     5341 agcccaagtc gatattgagg ccgtgagcga tggctcggcc gtgagcattt ccgtgcgcga
     5401 ttacggtccc gggattgaaa gcaaacacct ggaccggctg ttcgagcggc tctaccgggt
     5461 ggacaagggg cgcagccgtg aattgggcgg cacgggactg gggctggcga tcgtcaagca
     5521 tatcgccctt atccacaacg ggagcgtgga tgttgagagc aaggtcggca agggctcggt
     5581 tttcaccatc cggctgcctc ttcagtgtcc ggaccatgaa acacaagaaa tcaagataga
     5641 cagagagccg ggagcgcagg tgtcctcgcc tgcaggaata gttcgttagc gccttgccgg
     5701 ccgataaggc caataaaagg ttgtttctca cacgaagagc gggagaagtt cgttagttaa
     5761 ttagtgcatt agttaattag tgcattagtt cattagtgca ttagtgcgtt ggtacgttag
     5821 tgcgttacgg cttaacaagg tttgtacagc cttttggttg cgggctccgc ccgcgttggg
     5881 gttaaggctg tgaggggtta aggctgtgag gga
//
LOCUS       DAOSRB010000172         5633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13086_length_5633_cov_7.565794, whole genome shotgun sequence.
ACCESSION   DAOSRB010000172 DAOSRB010000000
VERSION     DAOSRB010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13086_length_5633_cov_7.565794"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLZ74_08865"
     CDS             <1..329
                     /locus_tag="PLZ74_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98472.1"
                     /translation="GGAVYARQVTLTQNDAKGPVVLDLGGVGVTCEVAVNGEPARVLV
                     CPPWRVDLTDRVKAGDNQLAITVYNTLNNHYQTIPTRYRTPVEATPSGLLGPVTLHFG
                     AKAVPR"
     gene            470..3268
                     /locus_tag="PLZ74_08870"
     CDS             470..3268
                     /locus_tag="PLZ74_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98473.1"
                     /translation="MKNKSSLLILASLLFAGCATPMRTVFVAPDGNDANPGTKSRPFA
                     TLPRALSSVRDLRQAGLPSGPVTVYLRGGTYAVRKPILFTPDDSGTSNAPLTVASYPG
                     EQAVLSSGERLSGTWTQTPGKPYWQLDIPRARNSGWVFYSLFVNGQSRERARFPNWGG
                     KVLRAEGREPGGDPRQALRYFAGDVNPAWSNPTDIDVVLLCSWTPTIHRINEIVPERR
                     AVRFFSAHNRPVDAWERNFRYYLSNVFEALDQPGEWYLNRHTGTLYYYPMPGEEMASA
                     EVIAPVLRSNMVTFEGDPESGRLIEHLHFRDLAFRHLDGDMDKHNGVYRQGHMFLSSA
                     VVAHGLRHASFERCEFAQLGEYALELADGCRDVTVRQCHLWDLGAGALQLGVTDLRTL
                     LHGRDTSPNPDRPRGKTTGSKTPPHPMEDPLSNPRFVTGLVIDNNCIHRLGTIWHGCY
                     GIVNRFASHSRITHNEIYDIHWDAIGLDARWNWKGENYSSGTVVAYNHLHHLGLRYHT
                     DAAGIYQYGPLDTHIHHNLIHDAVAYPYICGFAGIYLDEQSRGAVVENNLVYNTEWFA
                     YFQHKGTDNIFRNNIGAFARDGFLGRGSLNETWKTNYLEASRNLYISSNAVAIRSGWQ
                     PGLRAPVLTRNLYHTLTTNTLTFAGKEFAEWQAEGYDKDSVTADPGCRNPAAFDFSLE
                     PNAPACRLIGFTPFTTEIRKAGLYGDKAWRTTASRYPARVPSAVWDEVDFRKLGHAFE
                     LDFNLMKPGDGPDVFRVTDQKGAGFAVTREVAGMTGAHCLKATDKPGLSQSFYPYMHL
                     APRGLKSGRVTFTFALRHPERGAASCIVEFRGAGQTDTGPSIAVGRDGAIRANGKPVG
                     TLMPGAWNRFEIAFTLGSNRTDGYTLSIRTPEGEKTLSLPFGKKTFEQVGWLGIILPD
                     HTEGCLYLDAITLAVDES"
     gene            complement(3396..4901)
                     /locus_tag="PLZ74_08875"
     CDS             complement(3396..4901)
                     /locus_tag="PLZ74_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98474.1"
                     /translation="MTKVWRSVLLIGVGLLVRDAWTAEQDPADLLAETILRKVDTVLA
                     PVRERASGLRPAAPDKALVRTEVWREWRMDESGALCQVCVTGEIWTAAIQQALETHGC
                     VRLPERGMPYYIDAPILLSSGQLFRADPQAEIRLKPNSNTCMIRNRNVVGMREGGVIP
                     PVPYDTGVVVEGGIWTTLRIGRKESNGNNRGHPSRTDRAFESGHGVVLMNHADGLILR
                     NLTIRQSQVHGLQLSNVRHFHVEGIRFEEHGRDGVHVHGASEYGVIRDVRGATYDDFI
                     ALNAWDWRNTTPAYGPIRHVLVEEVGGSAEDPLRRAGSAAIRLLPGTRTWADGSKTAC
                     PIEFCVLRRLTAIQTFKVYDQPNLELGRDRDFSDPIGTVRDLFIRSLNCPAPCSIEIA
                     AHVSNFSVEDVTFRFPPAEGYRLIRVGPKSGTYKPRSTDPSTWVELFSPDKDITVRAL
                     SLRRVRVETAGKCAEIEDPERRFVVFEDQRPNPDYPKTTPKGGRGRVIRLP"
BASE COUNT         1088 a         1847 c         1697 g         1001 t
ORIGIN      
        1 ccggcggcgc ggtctatgcg cggcaggtga cgctcacgca gaacgacgcg aaggggcccg
       61 tcgtgctcga tctcggcggc gtcggcgtca cctgcgaggt cgccgtcaac ggcgaacctg
      121 cgcgcgtgct ggtctgcccg ccgtggcggg tggacctcac ggaccgcgtg aaagccggcg
      181 acaaccagtt ggctatcaca gtctacaaca cactcaacaa ccactatcag acgatcccga
      241 cccgttaccg cacacccgtc gaagccacac cttccggcct gctcgggccg gtgacgctgc
      301 acttcggagc gaaggcggtg ccgaggtaaa ccaagccccg gggttccggg cgaaaacgag
      361 agggaaactg gaatggtcaa cgtaaagaca agccgggatt tccgacagtt tcgcttcggc
      421 ggggagaaaa ccggaatgaa caggatttat gattaaggac aggacaccta tgaaaaacaa
      481 gagttcgctt ctcattctcg cctcccttct ttttgccggc tgcgccacgc cgatgcggac
      541 ggtcttcgtc gcgccggacg gtaatgacgc caaccccggc acgaagtccc ggccctttgc
      601 gacgctgccg cgcgccctgt ccagcgtgcg cgatctacgc caggccggac ttccgtccgg
      661 accggtcacc gtatatctgc gcggcgggac ctatgcggtg cgcaagccga tcctcttcac
      721 gccggatgat tccggaacct ccaacgcgcc gctgacggtt gcctcctatc ccggcgaaca
      781 ggcagtccta agcagcggtg aacggctcag cggcacatgg acgcagacgc cgggcaaacc
      841 ctactggcaa ctcgatattc cgcgtgcccg caacagcgga tgggtttttt attcgctctt
      901 tgtcaacggc caatcgcgcg aacgtgcccg cttcccgaac tggggcggaa aagttctgcg
      961 cgccgagggc cgcgagcccg gcggcgaccc gcgccaggcg ctgcgttatt tcgcgggtga
     1021 cgtgaacccg gcctggtcga atccgaccga tatcgacgtg gtgctgctct gctcgtggac
     1081 gcccacaatc caccgtatca acgagatcgt tcccgagcgc cgcgcggtgc gctttttcag
     1141 cgcccacaac cgtcccgtcg atgcctggga gcgcaatttc cgttactacc tcagcaacgt
     1201 gttcgaagcg ctcgaccagc cgggcgagtg gtatctcaat cgccacaccg gaacgcttta
     1261 ctattatccc atgccgggcg aggaaatggc ttcggctgag gtcatcgcgc cggtcctgcg
     1321 ctccaacatg gtcacctttg agggcgatcc cgaaagcggg cggcttatcg aacatctgca
     1381 tttccgcgac ctcgcgttcc gccaccttga cggcgacatg gacaaacaca acggcgtcta
     1441 ccgccagggc cacatgttcc tgtcctctgc ggtcgtcgcg cacgggctgc gccatgcctc
     1501 gttcgagcgc tgcgagttcg cgcagctcgg cgagtacgcg ctggagttgg ccgacggctg
     1561 ccgtgacgtg acggtgcggc agtgccacct ttgggacctc ggcgcgggcg cgctccaact
     1621 cggcgtcacc gacctccgta cgctgctgca cggccgggac acttccccga acccggaccg
     1681 tccgcgtggc aagacaacgg gttcgaagac cccgccccac ccgatggagg atcctttgag
     1741 caacccgcgt ttcgtgacgg gcctcgtcat cgacaacaac tgcatccacc gcctcggcac
     1801 gatttggcat ggctgctacg ggatcgtcaa ccgcttcgcc tcgcactcgc ggatcacaca
     1861 caacgagatc tacgatatcc actgggacgc catcggcctc gacgcgcgct ggaattggaa
     1921 aggcgagaac tattcctccg gcaccgtggt ggcctacaac cacctgcacc acctcggact
     1981 gcgttaccat accgatgcgg cggggatcta tcagtacggt ccgctcgaca cgcacatcca
     2041 ccacaacctc atccatgacg ccgtggccta tccctacatc tgcgggtttg ccggcatcta
     2101 tctggatgag cagtcgcgtg gcgcggtggt ggaaaacaac cttgtttaca acaccgagtg
     2161 gttcgcctat ttccaacaca agggcacgga caacatcttc cgcaacaata tcggggcctt
     2221 cgcccgcgac ggctttctcg gtcgcggcag cctgaatgaa acctggaaga ccaattatct
     2281 ggaggccagc cgcaatctct acatcagcag caatgcggtg gcgatccgca gcggctggca
     2341 acctggcctc agggcgccag tcctcacgcg caatctctac cacacactca ccaccaacac
     2401 gctgactttt gcgggcaagg agttcgcgga atggcaggcc gaggggtatg acaaagattc
     2461 ggtgaccgcg gatccgggtt gccgcaaccc cgccgcattt gatttctcgc tcgagccaaa
     2521 cgcacccgcc tgccgcctga tcgggttcac gccgttcact accgaaatcc gcaaggccgg
     2581 tctttacggc gacaaggcct ggcgcaccac ggcgtcccgt tatccggcgc gtgtgccctc
     2641 cgccgtgtgg gacgaggtgg acttccggaa actgggtcat gccttcgaac tggacttcaa
     2701 cctgatgaaa cccggcgacg ggccggatgt cttccgggtg accgatcaga agggggcggg
     2761 gttcgccgtc acgcgcgagg tcgccggcat gaccggcgcg cactgcctaa aagccacgga
     2821 caaaccggga ctctcgcaat cgttctaccc ctacatgcat cttgcgccac gcgggctgaa
     2881 gagcgggcgc gtcaccttca ccttcgcgct ccggcatccg gagcgtggcg cggcctcctg
     2941 catcgtcgag ttccgcggcg cgggtcagac cgacaccggg ccctcgatcg ccgtagggcg
     3001 cgacggcgcg atccgggcga acggcaaacc ggtcggcacg ctcatgcccg gcgcatggaa
     3061 ccggtttgaa atcgcgttca cgctgggctc aaaccggacg gacggctata cactcagcat
     3121 ccgtacccct gaaggcgaga aaacgctgtc cctgccgttc ggcaaaaaaa cgttcgaaca
     3181 ggtgggctgg ctcggcatca ttctgccgga tcacaccgaa ggctgtctct atctcgacgc
     3241 gatcacgctc gcggtcgatg agtcctgaac ccgggccaaa cggagacccc gcgtttcacc
     3301 cctggccctc cggattacgg gcaaacagac ggtctcctcc agcacggcgg gacggctacg
     3361 ctacgcgccg taggcgcgcc actttggttt caggtttagg gcaaccggat gacgcggccg
     3421 cggccgccct tcggggtggt tttcggataa tcgggattgg gccgctgatc ctcaaacacg
     3481 acgaagcggc gctcggggtc ctcaatctcc gcacacttgc ctgcagtttc gacacggaca
     3541 cggcgcaggg agagagctcg gacggtgatg tccttatccg gggaaaacag ttccacccaa
     3601 gttgaggggt ccgtcgagcg gggcttgtac gtgccggatt tcgggccgac ccggatcagg
     3661 cggtatccct cggcaggcgg aaaccggaat gtgacatcct ccaccgagaa attgctgaca
     3721 tgcgcggcga tttcgatgct gcaaggggca gggcagttca gcgaccggat aaagagatcg
     3781 cgcaccgtcc cgatcggatc actgaaatcc cggtcgcggc ccagttccag attcggctga
     3841 tcatagacct tgaaggtctg gatcgcggtg agtcgacgca acacgcagaa ctcgatcgga
     3901 cacgcggtct ttgagccgtc cgcccaagtg cgcgtcccag gcaggagacg gattgccgcg
     3961 cttccggccc tccgcaacgg atcctccgca ctgccgccga cctcttccac cagcacatgc
     4021 cggatcggtc cgtaggctgg cgtggtgttg cgccagtccc aggcattgag cgcgatgaaa
     4081 tcatcgtagg tggccccgcg gacatcgcgg atcaccccgt actcagaggc accgtggaca
     4141 tgcacgccat cgcgcccgtg ctcttcgaaa cggatcccct ccacgtgaaa gtggcgcaca
     4201 ttcgagagtt gcagcccgtg aacttgggac tgccggatcg tgaggttccg gaggatcagt
     4261 ccgtccgcat gattcatcag caccacaccg tgtccgcttt cgaacgcgcg atccgttcgg
     4321 gacggatggc cgcggttgtt gccgttgctc tccttgcgtc cgatgcggag cgtggtccag
     4381 atgccgccct cgaccacgac gccggtatcg tacggcaccg gcggaatcac gccgccctca
     4441 cgcatgccga ccacgttccg gttgcggatc atacacgtgt tcgagttcgg tttcagacgg
     4501 atctcggctt gggggtctgc acggaagagt tggccggagg agagcaggat cggtgcgtcg
     4561 atgtaatacg gcatcccccg ttccgggagc cgcacgcatc cgtgcgtctc gagcgcctgt
     4621 tgaatcgcgg cagtccagat ctccccggtc acgcatacct gacacagcgc accggactca
     4681 tccatacgcc actcccgcca gacctctgtg cgaacaagcg ccttgtccgg tgcggcagga
     4741 cgcagaccgg acgcgcgctc acgaaccggc gcgagaaccg tgtcaacttt ccgaagaatg
     4801 gtttccgcca acaggtcagc cggatcctgt tcagccgtcc aggcatcccg gacaagcagg
     4861 cccacaccga tcaacagcac gctcctccat accttcgtca tcgtcccccc ttttttgatg
     4921 aaggttgcct gcgtaaccgt tgggatctcc ccgcacacac gcgacaaccg taatgactgt
     4981 atccgaacct ccccgcaggg tcaagatggc cagcatgcga agagcgtgaa tccagatcga
     5041 atacatacgg ccggctgatg gaaaaagctt tgttttcgac aaaagcgggg ttgcgcggga
     5101 ttcggcaaaa gagtaaaatg aaagcgtgaa cgaaaggagc gttcccgtct tgtgcgggga
     5161 gaaacacggc atgtagaggg tgattccaga ccggttccga ttttgcgctt tcgcgcatta
     5221 cgcttctcaa gaaaaccgct aattttcatc aagcgaagcg agatacgcgg ggacgcgccc
     5281 ctgtggcgcg gcccctgtct cgggcttcgc ttagggtctt agcggtccct cttgaggggc
     5341 acgaaccggg gtccgcgccc tttactttat atgccgggaa agggacggca atggaaagcg
     5401 ataggacaag taacagaaag aaaagccttg tgcttcttgt cgcggcggcg tgcgcgtccg
     5461 cgggagtcat cttcgcatcg agacacgcgt tggcacagga aaacgcggag caggcacccg
     5521 cctcccgccc cgcttccgcg tgcaagacgc ccgccacccc gctggagatg gctctgaagc
     5581 tggagcgcga gctggcgcgg ggcgcggggg cgctcatgcc ggacccggag cgg
//
LOCUS       DAOSRB010000173         5604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13184_length_5604_cov_7.319697, whole genome shotgun sequence.
ACCESSION   DAOSRB010000173 DAOSRB010000000
VERSION     DAOSRB010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13184_length_5604_cov_7.319697"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..923
                     /locus_tag="PLZ74_08880"
     CDS             138..923
                     /locus_tag="PLZ74_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98475.1"
                     /translation="MNSLWGNHICEEGRDYTASVGCLRLTVRRIRQGWLVASRHTTDS
                     AFESLREVPAVPEQTEWMRFITPEAAPKLHLTPILPDRSVVSQSEAHIEIAPKHSGLF
                     YVTIPVWVQVRAGEISPVTLCEFPSQILSKTWFGHPSEEGEICYALTTRARQELAELT
                     DIQGRVICPLTLDNMTSEPLVFGQLLLSVRHMSVYQTQDGRLWTNECRMGFAGRSLPA
                     SVTFGRTVPAVAQQARWVSGPREAPASGLSARVIANAIFRQPF"
     gene            946..1779
                     /locus_tag="PLZ74_08885"
     CDS             946..1779
                     /locus_tag="PLZ74_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOR98476.1"
                     /translation="MNMTKINAVLGEYVLPFLGICAWLIFSLWFASFLARLLYNALSR
                     KVGPDTARVISKTVKYVLVVLFTLSLVRGAGVDVSAVLGAAGVAGVAIGFASQTSLSN
                     LISGLFLLLERPFKVGDWIEVDGSFGFVHSVELLSSYIRTFDNRMVRIPNEMLAKSKL
                     INHTHYPIRRLDLTIHVSCREDAARVMRLFREVVIANPLCFNEPEPMIVLQGFGDSSM
                     EFLVAVWTERSELRTTRTSLLRDIKERFDQEGIALPYHRIRMAECDGMAVPEGSGKEV
                     T"
     gene            1776..4172
                     /locus_tag="PLZ74_08890"
     CDS             1776..4172
                     /locus_tag="PLZ74_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR98477.1"
                     /translation="MRRLLKDEGVRLACVSAGGSVMAGVWLAEGLRVLDTFAGRTAPA
                     LAACAAVGVVGLTTGVLAAARPARRVRHAARWMSGTLLMCAVALVTLLAILPAMSAGW
                     QRLVGDLSRTFGDYLLAVGKTALVLFFVPAVLAGVAARFALAAVRTERTGAGQELTLC
                     LLLGLLPAVFGFGFGRTVLASAVDTETLLRLGALWCGALASFLVHGRGLSAALPFAAV
                     AVLLAAVRPATGPGVLAEGLFARLVHRDSGFARGTPVFAHRTRHHTVALFRDDDYETV
                     LTLDGRPVLFGNRFHTARTLTGLLPALLHPASAKVVVLGPEAGLYLPFVFRAGVTNVA
                     VAGSDRAVVELALAADRLVTGGDAVDVRQVKRRVRLTRRAGYDLIFLAGEPLWLRGTR
                     GSVDAKLFKRCRAALSDDGIVALHLDARALPMGRFVSVARRFTAVFSEVQVWQTGSDD
                     WLLIGATRPFEVRAERMLERFERREVMRDCARAGIRSLPEILACRVGNGPAWAAWLER
                     ASGSSVKAGIWRAPRTAFSSGAADRLRAASLDDLRPADLDWLRPGALDDATRAALLDK
                     AGVCREAHRLAASAFLLREQGRGEAALQAAREAARLHPGGVLLTPFGESLDLEGRRRI
                     AIGDFKGALKCYENLLSLQTGLARAHYGMAYCLRATGEAESAYLHFARAVAAAPTQIS
                     YRLAFAQACSAVGEYAEADRQYEAALRQEPDNPELLFRFARGLALAARPEKDFARAIR
                     LAERACVVTRWRHSEYAYGLADLYLDAGRVLEGMGLKRRLKEGLISEPGVPDGKGGTR
                     "
     gene            4169..5191
                     /locus_tag="PLZ74_08895"
     CDS             4169..5191
                     /locus_tag="PLZ74_08895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOR98478.1"
                     /translation="MNTAVAVFSALSPERVLEAVECGLRCRCTGLLRPLPSYINRVYT
                     VELETGETVVAKFYRPGRWTRAAIIAEHDLVLACAADEIPVIAPLPLADNKGTVGDAD
                     GIAFALYPRRGGRPIEPAEPADALWGRLGALLARVHRVGARAPAPDRLTLHPLSTTTE
                     DLAYLLDSGFLEAREAGRLEAFAAALFERIVPQFEELGGEVIRLHGDCQHTNVLERPE
                     TGLFLIDFDDMMNGTPVQDLWMLLPGRAAECAVEWAQMIEGYETFRAFDRRTVRLIEP
                     LRAMRMIYYLAWCARQAHDASFRQNHPDWGTRAFWVSELAELEAQLGVIGQDVAHSCH
                     IDREGQ"
     gene            5341..5434
                     /locus_tag="PLZ74_08900"
     tRNA            5341..5434
                     /locus_tag="PLZ74_08900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5375..5377,aa:Ser,seq:gct)
BASE COUNT          847 a         1581 c         1981 g         1195 t
ORIGIN      
        1 aagcatacgc cgtttataca tcggcgtcaa ccgctgtttc ggaaaacaac cgggaacttc
       61 acttgtcaca ttgaggttcc cggttattaa tgatcactga cattggccgc tcgcacggaa
      121 cctgctatac tttccgcatg aatagtctgt gggggaatca tatctgcgaa gaggggcgcg
      181 attacaccgc ctcggtcgga tgcctgcgcc tgactgtgcg gcgcatacgg cagggctggc
      241 tggtcgcgtc gcggcatacg accgacagtg ccttcgagtc gctgcgtgag gttccggctg
      301 tgccggaaca gaccgaatgg atgcgcttca tcaccccgga ggccgcgccg aagctgcatc
      361 tgacgccgat tctgcctgac cggtcggtgg tcagccagtc cgaggcgcat atcgagattg
      421 cgccgaaaca cagcggcctg ttttacgtca ccattcccgt gtgggtgcag gtgcgggccg
      481 gtgagatttc acccgtaacg ctgtgcgagt tcccttccca aattctttcc aaaacgtggt
      541 ttggtcatcc ttcggaggag ggggaaatct gttatgccct gaccacccgc gcgcgtcagg
      601 agcttgcgga actgaccgat atccagggcc gcgtgatctg tccgctgact cttgacaaca
      661 tgacctcgga gccgctggtg ttcggccaac ttttattgag tgtgcgccac atgagtgtct
      721 atcagaccca agacgggcgg ttgtggacga acgagtgccg catggggttc gcgggccggt
      781 ctcttcctgc gtccgtgacc ttcggtcgca cggtcccggc tgtcgcgcag caggctcgtt
      841 gggtctcagg tccccgcgaa gccccggcct ccggcctgag tgccagagtg atcgccaacg
      901 cgattttccg ccagccgttc tgagtcgaaa aagggaggga agggcatgaa catgacgaaa
      961 atcaatgccg tgctggggga gtatgtgctc ccgttcctcg gcatctgcgc ctggttgatc
     1021 ttcagtctct ggttcgcctc gtttctggca cgtctgctgt ataacgcgct ctcccgaaaa
     1081 gtggggcccg ataccgcgcg cgtgatttcg aaaacggtca aatatgtgct tgtggtgttg
     1141 tttacgctct cgctggtccg cggcgcgggt gtggatgttt cggcggtgct gggtgccgcg
     1201 ggcgttgcgg gtgtcgccat tggcttcgcc tcccaaacca gtctttcgaa tctgatcagc
     1261 ggcctgttcc ttctgttgga gcggccgttc aaggtggggg actggattga ggtggacggc
     1321 tcgttcggct ttgtccatag cgtggaactg ctctcctcct acatccgtac gttcgataac
     1381 cgtatggtgc gtatccccaa tgagatgctg gccaagtcca agctcatcaa tcacacccat
     1441 tatccgatcc gtcggctgga tctcacgatc cacgtttcgt gccgggagga tgcggcgcgg
     1501 gtcatgcggc tcttccgtga ggtggtgatc gcgaatccgt tgtgcttcaa cgagcctgaa
     1561 ccgatgatcg tcttgcaggg gttcggcgac tcgtcgatgg agtttctggt ggcggtctgg
     1621 accgaacgga gcgaactgcg gaccacgcgg acatccctgt tgcgggatat caaagaacgg
     1681 ttcgatcagg aagggatcgc gttgccgtat caccggatcc gcatggcgga gtgcgatggc
     1741 atggccgtgc cggagggaag cgggaaagag gttacatgag gcggcttctg aaggatgagg
     1801 gagtgcggtt ggcctgcgtg agcgcgggcg gcagtgtcat ggctggcgtg tggctggccg
     1861 aggggttgcg ggtgctggat acctttgcgg gccgcactgc gcctgcgttg gccgcctgtg
     1921 ccgccgtagg ggttgtcggc ttgacgacag gggttctcgc cgctgcccgt ccggcgcgtc
     1981 gggtgcggca cgccgcaaga tggatgagcg gcacgttgtt gatgtgcgcg gtcgcgttgg
     2041 tgacgctgct cgcgatcttg cccgcgatgt ccgcgggatg gcagcgtctg gtgggtgact
     2101 tgtcgcgcac gttcggcgac tacctgttgg cagtcggcaa gacggccctc gtgctgtttt
     2161 tcgttccggc ggtgctggcc ggtgtggccg cccgctttgc gttggcggct gtccggacag
     2221 agcgcacggg cgccggacag gagctgacgc tgtgcctgct gctcggtctg ttgcctgcgg
     2281 tgttcggctt tgggtttggc cggacagtgc tggcatccgc cgtcgatacg gaaacgttgt
     2341 tgcgtctggg cgcgttgtgg tgcggtgctc tggcctcgtt cctcgtgcac ggccggggat
     2401 tgagtgccgc gttaccgttc gcggcggtgg cagtgctgtt ggcggcagtg cggcccgcta
     2461 cagggccggg agtgctggcg gagggtctgt tcgcgcgtct ggtgcaccgg gacagcggtt
     2521 tcgcgcgcgg cacgccggtg ttcgcgcacc ggacgcgcca tcataccgta gctttgtttc
     2581 gagacgacga ttacgaaacc gtgctgaccc tcgacggtcg gccggtgctg ttcggtaacc
     2641 gtttccacac ggcgcgcaca ctgaccgggc ttctgcctgc gctgctgcat cccgccagtg
     2701 ccaaagtggt ggtgctgggt ccggaagcgg ggctgtatct gccattcgtt ttccgtgcgg
     2761 gtgtcaccaa tgtggcggtg gcggggtccg atcgggcggt cgtggaactg gctctggctg
     2821 cggaccggct tgttacgggc ggggatgcgg ttgatgtgcg gcaagtgaag cgcagggtcc
     2881 ggctgacacg gcgggcgggg tatgatctca tttttctggc cggcgaaccg ttgtggttgc
     2941 gcgggactcg cggctcggtg gacgcgaaac tgttcaagcg ttgccgcgcg gccctctcgg
     3001 acgacgggat cgtggcgttg catctggatg cgcgggcctt gccgatgggg cggttcgttt
     3061 cggtggcccg gcgtttcacc gcggtgttca gcgaggtgca ggtgtggcag accggttccg
     3121 acgactggct gttaatcgga gccacgcggc cgttcgaggt gcgtgcggaa cgcatgctcg
     3181 agcggttcga gcggcgtgag gtcatgcgcg attgcgcacg ggcggggatc cggagtctgc
     3241 ctgagattct ggcgtgccgg gttggcaacg gtcctgcatg ggcggcttgg ctggaacgcg
     3301 cgtcgggttc ttcggttaag gctgggatct ggcgcgcgcc gcggacggcg ttttcgtcag
     3361 gggcggcgga tcggttgcgc gccgcgtcgc tggatgactt acggcccgcg gatctcgact
     3421 ggctgcggcc gggcgcactc gatgacgcca cgcgcgcggc cctgctggac aaggccggtg
     3481 tctgccggga ggcgcaccga ttggcggctt ccgcattcct cttgcgcgaa cagggtcggg
     3541 gcgaggccgc tctgcaggcg gcacgcgagg cggcgcgcct tcatcctggc ggcgtcttgt
     3601 tgactccctt cggcgaatcg ctggatctcg aggggcgccg acggatcgcg atcggcgatt
     3661 tcaaaggcgc gttgaagtgt tacgagaacc tgctttcgct tcagacaggg ctggcgcgcg
     3721 ctcattatgg catggcctac tgtctgcggg ccaccggtga ggcggagtcc gcatacctcc
     3781 atttcgcgcg tgcggtggct gcggcaccca cgcagatttc ttaccgtctg gctttcgcgc
     3841 aggcctgttc cgctgtgggc gagtatgcgg aggcggaccg gcaatatgag gcggcgttgc
     3901 ggcaggaacc cgacaatccg gaattgctgt tccgtttcgc ccgggggctc gccctcgccg
     3961 cgcgacccga aaaagatttc gcgcgggcca tccgtttggc cgagcgcgcc tgcgtggtga
     4021 cgcgctggcg gcattcagag tacgcctatg ggcttgcgga cctctatctg gacgctggcc
     4081 gcgtactgga gggcatgggc ctgaaacggc gtctgaagga ggggttgatc tccgagcccg
     4141 gcgttcccga cggaaaggga gggacgcgat gaatacggct gttgcggtgt tcagcgcgct
     4201 ctcgccggaa cgtgtgctgg aggcggtgga gtgcggtttg cgctgtcgtt gcaccgggct
     4261 gttgcgtccc ttgccgagtt acatcaaccg cgtgtacacg gtcgaattgg agaccgggga
     4321 aacggtggtc gccaaattct accgtcccgg ccgctggaca cgtgcggcga tcattgcgga
     4381 gcacgacttg gtgttggcct gtgccgccga cgagattccc gtgatcgcgc cgttgcctct
     4441 cgccgacaac aagggaacgg tgggggatgc ggacggtatc gcgttcgccc tgtatccgcg
     4501 gcggggcggg aggccgatcg agccagcgga gccagccgac gcgctctggg ggcgtcttgg
     4561 tgccctgctg gcgcgtgtcc atcgggttgg cgcccgggcg cccgcgccag accggctgac
     4621 gctgcatccg ttatccacca caacagagga tctcgcgtac ctgctggaca gcgggtttct
     4681 ggaggcgcgt gaagccgggc gtttggaagc ctttgccgca gctctgttcg aacggatcgt
     4741 gccgcagttt gaagaactcg ggggtgaggt catccggctt catggggact gccagcacac
     4801 caatgtgctg gagaggcccg agaccggctt gtttctgatc gattttgacg atatgatgaa
     4861 cggtacgccc gtacaggact tatggatgct gttgccgggc cgggcagcgg agtgcgcggt
     4921 cgaatgggcg cagatgattg agggatatga gaccttccgc gcgtttgacc gacgtaccgt
     4981 gcggttgatc gagcccctac gcgcgatgcg catgatttat tatctggcct ggtgtgcgcg
     5041 gcaggcccat gacgcctcgt tccggcagaa ccatccggat tggggtacgc gcgccttctg
     5101 ggtttcggag ctggctgagt tggaggcgca actcggagtg attggacagg atgtggcgca
     5161 ttcgtgccat atcgaccggg aaggccaata aaaaacaagt tgtttctcac acgaagcgaa
     5221 gggcacgaag gagaatgggg tgtcatgaaa aacctttgat caacggtttt gcggtttacg
     5281 cttgaaccgg ttggtcttct tttcgtatga tgactcccgt tttcaaacca tacgtgttct
     5341 ggagaggtgg ccgagtggct gaaggcagtg gtttgctaaa ccgccgtatc ggatgaaacc
     5401 ggtaccgggg gttcgaatcc ccccctctcc gccaattccc cttgccctcg aatcaggagg
     5461 gggggggatg agaacccccg ggttcgaccg cctgcgcagc aggcaggggc cgcaccgaag
     5521 cgtgcggcag ggaggcgcag ccgaccggca atccccccct ctccgccaat tccccttgcc
     5581 ctcgaatcag gagggggggg gatg
//
LOCUS       DAOSRB010000174         5602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13192_length_5602_cov_6.693889, whole genome shotgun sequence.
ACCESSION   DAOSRB010000174 DAOSRB010000000
VERSION     DAOSRB010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13192_length_5602_cov_6.693889"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLZ74_08905"
     CDS             <1..686
                     /locus_tag="PLZ74_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98479.1"
                     /translation="HLQGIATDESGNLYWSWTTFLVKTAATGTLLAKVPVPTHYGDLT
                     WHGGKVYVAVNFGQFNKEPGKAKSWVCVHDAETLALLSSNAVPEVVHGAGGIEWHNGH
                     FFVVGGLPATHTANYVYEYTGSFEFVKRHVIESGQTFLGIQTVCRMRDGTWWFGCYGK
                     PAVVLKTDDRFTFCGKYRFNGAVGMARTPKNDELLIATNRLIAKRNSASVRPVKTETI
                     TARIQEDKH"
     gene            791..2290
                     /locus_tag="PLZ74_08910"
     CDS             791..2290
                     /locus_tag="PLZ74_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOR98480.1"
                     /translation="MRYAIVSDIHANFAAWRTVLADIADIRADQIICLGDMVGYGPQP
                     VEVLESVYRTVQVTLMGNHDAAVCGRLDPGLFLPHARAAVLRHRAQLSAAGIAWLERL
                     PLVLTAPTFRCTHGDFSDPSAFYYILDEQDACPSWRATQEPLLFAGHTHMPGIYVSGA
                     SGQPHFLEPRDFTLEPGKRYIVNPGSVGYPRTGPFRSSYCLYDDEAGTVVFRQLPFDD
                     EAYRAALREAGLENSAPWLERLTLHRYRPAVRVRFSFANPVTASQSGRVSARRYGATG
                     RRLARNLALGLLALGAVGVAGRLIFRAGHATAESAPVAMMPPFELPVRAAYPLMHSGG
                     NLLPPLPESVAPDRRLDGWRYALEDRDRQRFALGLRDGGLTLVVRHDGPRRFQLESPL
                     IDLAGTRITALRLTSRVRKPGPFEGSATIQLVTYTRLPHDLLQRGRTEQYELRTTRRR
                     LVPPGAERNVRIRLGRAVTHVRFRVEATFDGVLEIEQPALRAEQEGHLP"
     gene            2287..3150
                     /locus_tag="PLZ74_08915"
     CDS             2287..3150
                     /locus_tag="PLZ74_08915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR98481.1"
                     /translation="MIQRVSEEVWAFDCEWVPDLQAGRLLYRLPEDLPSQEVLRVMWE
                     QGGATPENPTPFLKTILCRVVSVATVIRRVSRNGEVRLFLQALPEAPDDPSQDEAHIL
                     RRFLVDGMAAHNPQLVGFNSRNADLRILTQRALVLGLPQPALCGRLAAKPWESLDVDL
                     MDRISGYGKTYAVSLNELATLSGIPGKLDTCGDDVCGLWHSGQRRKIVEYNCFDALTT
                     YLVWLRMAYFSGCFDDEGYRTEQRRLYTLLRERLLKPEDAYLRAYLDAWRDLKRRTGQ
                     LDDGVFPPDDQ"
     gene            complement(3144..3923)
                     /gene="lpxA"
                     /locus_tag="PLZ74_08920"
     CDS             complement(3144..3923)
                     /gene="lpxA"
                     /locus_tag="PLZ74_08920"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOR98482.1"
                     /translation="MASIHPTAIVDPKAELDTDVEVGPYATIGPNVRIGAGTVVQSHA
                     YVVGHTTLGKNCQVFPFACVGMKTQDLKYQEGDVTYVEVGDETVLREFSTVHLGTKPG
                     EVTRVGSHCLIMAYCHIAHGCVVGNHVIMANVTTLAGEVTVEDFVTIGGMVGVHQFCR
                     IGTYAMIGGATKVRQDCPPYMLTENVGSDTHVLGPNIVGLQRRGFSVETRSALKEAFK
                     LIYREGLNRTQALERIKYEVADLPEIQTLVAFYQNSRRGVH"
     gene            complement(3953..5044)
                     /locus_tag="PLZ74_08925"
     CDS             complement(3953..5044)
                     /locus_tag="PLZ74_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOR98483.1"
                     /translation="MKILERYVLQAFLSAFALAWLILSFVMTIGLLVRITQLMIEGLP
                     PRAVGLFMLVGFPETLRLTLPLALLVSALLVFSRLSADNEISAMRACGVNLLAIMRWP
                     LVTGALCTLIGLYINNEIVPRSHEVRRNLRSVISVDVGLDLLESGRFITDFPKMTLWF
                     ARKEGNWIQDLIVFDHSQEGLTREVRADKALVTTNGTDIVLEMYKVRIDPIEVDRPGV
                     ASADRFRHVIPEAMKRRRYVRKEKDFHFNELRQAIRKVRANEKGLSRKALQRLLSIYR
                     TEYRMRYVYAFASLCFVMIGVPLGIRTHRKESTIGMAVSLVVSMAYYLCVILLQSLDK
                     YPALRPDILLWLPVGVCLTLATWLISKNL"
     gene            complement(5133..>5602)
                     /locus_tag="PLZ74_08930"
     CDS             complement(5133..>5602)
                     /locus_tag="PLZ74_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR98484.1"
                     /translation="DRDTDKGGVADGHEVIEDKTNPLDPSDDLMLFEVYIQFDYDTTV
                     IKPQYFSEIDQVVRVLQRNTKATAVIEGHADRRTRSSEKYNQDLSERRAQAVQSYMVG
                     KGVAVQRLSAVGYGYSRPKVQPDLVNGNPENRRVEIYIRGAGTNADKVKYVNP"
BASE COUNT         1056 a         1775 c         1670 g         1101 t
ORIGIN      
        1 ggcatctcca aggcatcgcc acggacgaaa gcgggaacct ctattggtct tggaccacgt
       61 ttctggtcaa gactgccgca accggcacgc ttttggcaaa agtgcccgtt cccacgcatt
      121 acggcgatct gacatggcac ggcggcaaag tctatgtggc cgtgaatttc gggcagttca
      181 acaaggagcc ggggaaagcc aaatcatggg tctgtgtgca tgacgcggaa acgctcgcct
      241 tgctctcgtc gaacgcggtg cccgaggtcg tacatggcgc gggcggaatc gagtggcata
      301 acgggcattt ctttgtggtc ggcgggctgc ctgcgacaca cacggccaac tatgtgtacg
      361 agtatacggg atccttcgaa ttcgtgaaac gccatgtgat cgaaagcggg cagacctttc
      421 tgggcatcca gaccgtttgc cgcatgcggg acggcacctg gtggttcgga tgttacggca
      481 agcctgcggt cgtattgaaa acagacgacc ggttcacgtt ttgcggcaaa taccgtttca
      541 acggcgctgt cgggatggcc cggaccccaa agaacgatga attactgatc gccacaaacc
      601 gcttgatcgc aaaacgcaac tcggcatcgg tccggcccgt caaaacggaa accatcacgg
      661 caagaattca agaagacaaa cactgagggg tagacacgcc ggaaggacat catcccgcag
      721 accttttgcc ttgaacagcc ttggttcacg cattaaacta atttgtgttg tccatacgtt
      781 tcctttactt atgcgttacg cgatcgtttc cgacatccac gccaatttcg cagcctggcg
      841 gaccgtgctg gccgacatcg ccgacattcg ggcggatcag atcatctgtc tcggcgacat
      901 ggtcggctac ggtccgcaac ctgtggaagt gctggaatcg gtttaccgga ccgtgcaggt
      961 gacgttgatg ggcaaccacg acgcggcggt ctgcggacgg ctggaccccg ggctgttcct
     1021 gccgcatgcc cgcgccgccg tgctgcgcca ccgcgcgcaa ctctccgctg cgggaatcgc
     1081 gtggctggaa cggcttccgc tggtcctgac cgcgcccacg ttccgttgca cgcacgggga
     1141 tttctccgac ccgtccgctt tttattatat cctcgacgag caggacgcgt gcccgtcgtg
     1201 gcgggccacg caggaaccgc tgctctttgc gggacacacc cacatgccgg gcatctatgt
     1261 cagcggagcg agcggacagc cccattttct ggaaccgcgc gacttcacgc tcgaaccggg
     1321 gaaacggtat atcgtcaatc cgggcagtgt ggggtatccg cgcacggggc cgttccgttc
     1381 ctcctactgc ctgtacgacg acgaggccgg aaccgtcgta ttccgccaac tcccgttcga
     1441 cgatgaggct taccgcgcgg cccttcgcga ggcgggtctg gagaacagtg cgccgtggct
     1501 ggaacgcctg accctgcacc gttaccgtcc ggcagtgcgc gtgcggttct ctttcgcgaa
     1561 cccggttacg gcgagccaat cagggagggt gtccgcgcgc cgttacggtg cgacgggacg
     1621 gcggcttgca cggaacctcg cgctcggcct gctggcgttg ggggcagtgg gtgttgcggg
     1681 ccgcctcatc ttccgtgccg ggcacgcgac ggccgaatcc gctcccgtag ccatgatgcc
     1741 gcccttcgag ttgcccgtgc gggccgccta tcccttgatg cactccggcg ggaatctgtt
     1801 gccgccgctg ccggagtctg tcgctccgga ccggcggctc gacgggtggc gctacgctct
     1861 ggaggatcgt gaccggcagc ggttcgccct tggattgcgt gacggcggcc tcacgctggt
     1921 ggtacgccat gacggccccc gacggttcca gttggagtct ccgctgatcg atctggcggg
     1981 aacccggatc acggctttgc gcctaacctc gcgcgtgcgc aaacccggac cattcgaggg
     2041 ttccgccacg atccaactgg tcacgtacac ccgtttgccg catgacctgc tgcagcgggg
     2101 ccgtaccgaa caatacgaat tgcgcactac gcggcgtcgg cttgtgccgc ccggggccga
     2161 acgcaatgtg cggatccggc tcggccgcgc ggtcacgcat gtgcgtttcc gcgtggaggc
     2221 cacattcgac ggggtactgg aaatcgaaca accggccctc cgcgcggaac aggaaggaca
     2281 cttgccatga ttcaacgtgt ttccgaagaa gtttgggctt ttgactgtga atgggtgccg
     2341 gatctgcagg cggggcgctt gttgtatcgg ttgccggagg atctgccctc tcaggaggtt
     2401 ctgcgcgtca tgtgggaaca gggcggcgca acgccggaga acccgacccc gtttctgaag
     2461 acgattctct gccgcgtggt ttcggtggcc acggtgatcc ggcgcgtatc ccgcaacggc
     2521 gaagtacggc tctttctgca ggccctgccc gaagcgcccg acgacccgtc acaagacgag
     2581 gcccacatcc tgcggcgctt tctggtggac ggcatggccg cgcacaatcc gcaactggtc
     2641 ggcttcaatt cgcggaacgc cgacctgcgc atcctcacgc agcgcgcgct cgtcctcggt
     2701 ctgccccaac cggcactctg cggacgtctg gcggcaaagc cttgggagag tctcgacgtg
     2761 gacctcatgg atcggatcag cggatacggc aaaacctatg cggtctcgct aaacgagttg
     2821 gccaccctgt ccggcattcc gggcaagctg gacacctgcg gcgacgacgt atgcggtctc
     2881 tggcacagcg gccagcggag aaaaatcgtg gagtacaatt gtttcgatgc cctcaccacc
     2941 tatctggtgt ggctgcgcat ggcctatttc tcggggtgtt tcgacgacga gggctaccgc
     3001 accgagcagc gccgtttgta cacgctgctc cgcgagcgcc tgctgaaacc ggaagacgcc
     3061 tacctgcgag cctacctcga cgcatggcgc gacctcaaac gccgcaccgg ccagttggac
     3121 gacggcgtct tcccgcccga cgatcagtga accccgcgtc ggctgttctg gtaaaacgcg
     3181 accagcgtct ggatttccgg caggtccgcc acctcatact tgatgcgttc cagcgcctgt
     3241 gtacggttga ggccttcgcg gtagatcagc ttgaacgcct cttttagcgc ggaacgcgtc
     3301 tcaaccgaga aaccccggcg ctgcaacccc acgatgttcg gccctaaaac atgcgtgtcc
     3361 gaacccacgt tttccgtcag catataaggc ggacaatcct gccggacctt tgtcgcgccg
     3421 ccgatcatgg cgtaggtccc gatgcggcaa aactggtgaa cgccgaccat gccgccgatc
     3481 gtgacgaaat cctcgacagt cacttcgccc gcgagggttg tcacattcgc catgatcaca
     3541 tggttcccca ccacacaccc gtgtgcgata tggcaatacg ccataatcag gcaatggctc
     3601 cccacccgcg tcacttcacc cggcttggtc ccgagatgca ctgtggaaaa ctcgcgcagt
     3661 acggtttcgt cgcccacctc gacataggtg acatccccct cttggtactt caggtcctgc
     3721 gtcttcatgc cgacgcaggc gaacgggaag acctgacagt tcttgcccag cgtggtatgc
     3781 cccaccacat aggcgtgcga ctgcaccacc gtgccggccc cgatccggac attcggtccg
     3841 atggtcgcgt aggggccgac ctccacatcg gtgtccagtt cggccttagg gtccacaatc
     3901 gctgtcggat gaatactcgc catacgttcc tcctcctccg ttgttttaga tgtcacaggt
     3961 ttttggaaat gagccaggtt gctaaagtca gacagacgcc gaccggcagc cacagcagta
     4021 tgtccggccg cagtgccggg tatttatcca gcgactgcag caggatcacg caaagataat
     4081 aagccatcga caccaccaag ctgaccgcca tgccgatcgt ggattcctta cgatgggtgc
     4141 ggatgccgag cggcacgccg atcatgacga aacacaacga cgcgaacgca tacacgtaac
     4201 gcatacggta ttcggtgcgg tagatgctca acagccgttg cagcgctttg cgcgacaggc
     4261 ctttttcatt ggcgcgcacc ttgcggatcg cctgccgcaa ctcgttgaaa tggaaatctt
     4321 tttccttgcg gacataacgg cgtcgtttca tcgcctcggg gatcacatgc cggaaccggt
     4381 ctgcggaggc cacgccgggg cgatcaacct cgatcggatc gatccgcacc ttgtacatct
     4441 ccagcacgat gtccgtcccg ttggtggtga ccagcgcctt atccgcccgg acttcgcggg
     4501 tgagcccctc ttgactgtgg tcgaacacga tgaggtcttg aatccagttg ccctccttac
     4561 gagcgaacca gagggtcatc ttggggaagt ctgtgatgaa acggcccgac tccagcagat
     4621 ccagccccac atccacactg atcaccgagc gcaggttgcg gcgcacctcg tggctgcgcg
     4681 gcacaatctc gttgttgatg tacagaccga tcagcgtgca gagcgcaccc gtcaccagcg
     4741 gccatctcat gatggcgagg aggttcaccc cgcaggcccg catggccgag atctcattat
     4801 cggcggaaag gcggctgaac accagcaacg cactgacgag cagcgcgagc ggcagggtga
     4861 gccggagggt ttcgggaaag cccacgagca tgaagagccc gaccgcgcgg ggcggcagtc
     4921 cctcgatcat gagctgcgtg atccgaacca gcagcccgat cgtcatcaca aacgacagga
     4981 tcagccaggc cagtgcgaac gcacttagaa aggcctgcaa gacatatcgc tccagaatct
     5041 tcatgggcgc tattatacaa aaaaggccgc agaagaaacg gctgtttctt tgcggccttc
     5101 cgctggcaac gtttccgcgg acggcggaag cgttacgggt tcacatactt gaccttgtcc
     5161 gcgttggtgc ccgcgccgcg gatgtagatc tccacgcggc ggttctcggg attcccgttg
     5221 accagatcag gctggacttt gggccggctg tagccgtatc ccaccgcact cagacgctgc
     5281 acggctacgc ccttgcccac catgtaggac tgcaccgctt gggcccggcg ctcggagagg
     5341 tcctgattgt acttctcgct cgaacgcgtc cgccgatccg catggccttc gatcaccgcc
     5401 gtggcctttg tgttgcgctg cagcacgcgg acgacttggt caatctcact gaagtattgg
     5461 ggcttgatca ccgtggtatc ataatcgaac tggatgtaga cctcgaacag catcaggtca
     5521 tcggaggggt ccagcgggtt ggtcttgtcc tcgatcacct cgtgtccgtc cgccacgcca
     5581 cccttgtcgg tatcacggtc ca
//
LOCUS       DAOSRB010000175         5572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13301_length_5572_cov_7.100598, whole genome shotgun sequence.
ACCESSION   DAOSRB010000175 DAOSRB010000000
VERSION     DAOSRB010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13301_length_5572_cov_7.100598"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="PLZ74_08935"
     CDS             <1..1569
                     /locus_tag="PLZ74_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOR98485.1"
                     /translation="SRIGVVLAAAGSAVGLGNFLRFPGVAARNGGGAFMIPYFIAFFF
                     LALPLCWMEWAMGRYGGQFGHGSAPGVFDAVGGRRHPALKYFGVLGVTGALGIFFYYI
                     YIESWTLAYSVFALTGRYAAIETPDCMGAFLSSYLGGNGDFFQSRLSAYGFFLVTFAA
                     NFRVVYHGISHGIERFCKIAMPLLFLTAVVLVVRVFTLGAPVNPEWTLDRALGFLWNP
                     DFTALRSGKVWLEAAGQVFFSTSVGIGVLLTYATYLKPHDDALLSSTTASFLNEFAEV
                     VLGGSIVIPAAFLFFGPVGTTEAVEGGTFGLAFRTTPLIFNHMAGGAWVGFAWFALLF
                     IAGVTSSVSILQPAVAFLEEEFGFNRKRCVIVIGLLCFVVAHLAIFGDGVVDEMDFWF
                     SSFGLPMFGLIEVCVFLFFFGPSRGWDALHTGSVLRLPRFFKFVMTFITPVFLFAILA
                     GWLVTDGWKTILMRKTVDGQLVPLYTPEQVPWVLATRLFYLALVVGTCLLIRRAWAHR
                     NWVKHPSVEPQKSR"
     gene            1809..2720
                     /gene="folD"
                     /locus_tag="PLZ74_08940"
     CDS             1809..2720
                     /gene="folD"
                     /locus_tag="PLZ74_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOR98486.1"
                     /translation="MQATILDGKVLAAELRAEIARETAEFKRQSGLTPGLGVILIGAD
                     PASVSYVTAKEKACAEAGMVSQDVRVPAETTQAEVLALVNRMNEDSRIHGILVQLPLP
                     RHINESAVLNAISPDKDVDGFTPANVGRMILGEPCYLPCTPHGIIRMLLKAEIQTAGR
                     HAVVVGRSNIVGKPVAHLLMRKAEGGNATVTVCHTGTRDLAEHTRRADILVVAAGRPN
                     TVTGDMIRPGAVVIDVGVNRIPDPSRPSGFRLVGDVDFDSARRVASLITPVPGGVGPM
                     TITMLLHNTLEAARRAAAHTVPGGPNP"
     gene            2717..4675
                     /locus_tag="PLZ74_08945"
     CDS             2717..4675
                     /locus_tag="PLZ74_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98487.1"
                     /translation="MKRLGEPLFARDETGRLKTRIGTFFLRTPGLVTLPGVHATQRIA
                     WLNELNRERQVAGIPALSEAESDAEIACSVDLLFNDQHVLIRPDPYAVDLAFEADEAL
                     QALVSKRKIRFLNTQNEIIRNALRARGENWRMSRVPVSPEEMNRMIAQARVAISGLPL
                     YFHNAATGTRFLTLETFAWLGTQPDDVFRKHLAEIVRYSSLRNRFGYPEIDIFPVGCA
                     FSRQAFEALNAEGLEIGALRAAYRKLLAAFREAVPIEFHEETFENLDWRNRLCSTLIH
                     QPNAVATQDLPDGMAPEFHLQIEWLPGCRIDEGELIFDPVYEELDLMPEDAELRAFCD
                     PRAREIIFNYVREFTTIEFINVGRITRSLSRRVQTGEHRTDVYVVQIKETGKPKPELC
                     ILRFQKWGVCEHLDEGKDMVRSIMEAVDYTDYVLDRRLGCRQLGMNLPEKVSTGRMPT
                     LYTGSNSLYKGAKCWTVYFERQYIPGQVTDKILTRHYANPEFNLLLARLLGEAAVVNC
                     VVGRANTEGQVLFDDGDEVITLDQAGMPERLTVSDHTGAFTLYEEPLEQMAEAYADPV
                     NKRIALMPNPEAFAETYLASFRQRFVTLQQEYRRRRHAFDKLFKHRPVDPAGSLAYRW
                     QCILARLDATDAETLTEAIRSQIRGLHP"
     gene            4672..5409
                     /gene="nudC"
                     /locus_tag="PLZ74_08950"
     CDS             4672..5409
                     /gene="nudC"
                     /locus_tag="PLZ74_08950"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOR98488.1"
                     /translation="MKQCLCFRDERLWIRRSPAGTEWLFETNPNAQILPVEPLPDGTW
                     AAESESAPVPGAESIGLRELFDLVSAETSQNAGMARQLLHWRRSHRFCSVCAAPLERH
                     PTERAMYCLACGHLAYPRINPVAITLIHRGREVLLARKAGNQYPFWSLIAGFVEVGET
                     LEQTVRREVHEEVGLRVRDIRYATSQAWADPSNLMIGFTASYDGGEILPDGREIAEAD
                     WFGCSCLPPIPSRISIARRLIDAFFTA"
BASE COUNT          978 a         1765 c         1710 g         1119 t
ORIGIN      
        1 agccggattg gcgtcgtcct agcggcggcc ggcagtgcag tcggtctcgg caacttcctg
       61 cgtttccccg gcgtggccgc gcgtaacggt ggcggggctt tcatgatccc gtacttcatc
      121 gccttttttt tcctcgccct gccgctctgc tggatggagt gggccatggg ccgttacggc
      181 ggacagttcg gccacggcag cgcaccggga gtcttcgatg cggtgggcgg tcgccgccat
      241 ccggccctga aatacttcgg cgtgctcggt gtcaccggcg cgctgggcat ctttttctat
      301 tacatctata tcgaatcgtg gacactggcc tacagcgtgt tcgcgctcac cggccgctat
      361 gccgcgattg aaacgccgga ctgcatgggt gcttttctca gcagttatct gggtggtaac
      421 ggcgactttt tccagagtcg gctttccgcc tacggctttt ttctggtgac tttcgccgcc
      481 aacttccggg tggtctatca tggtatcagc cacggcatcg agcgcttctg taaaatcgcc
      541 atgccgttgc tcttcctcac ggccgtcgtg ctggtggtgc gcgttttcac gctcggtgcg
      601 ccggtcaatc cagagtggac actcgaccgc gctttggggt tcctgtggaa ccccgatttc
      661 accgcgctcc gtagcgggaa ggtctggctg gaagccgcag gccaggtctt cttctcaacc
      721 tcagtgggca tcggtgtatt gctgacctat gccacatatt tgaaaccgca cgacgatgcg
      781 ctgctctcct ccaccaccgc ctcgttcctg aacgaattcg ccgaagtcgt gctcgggggc
      841 tcgatcgtca tccccgccgc ctttcttttc tttgggccgg tcggcaccac cgaggcggtc
      901 gaaggcggaa ccttcggcct tgccttccgc accacgccgc tgattttcaa ccacatggcg
      961 ggcggcgctt gggttgggtt cgcctggttc gcactgcttt tcatcgctgg tgtcaccagc
     1021 tcggtctcga ttctgcaacc tgccgtggcc ttcctggagg aggagttcgg cttcaaccgc
     1081 aagcgctgcg tgatcgtcat cgggcttctt tgtttcgttg tggcacatct cgcgattttt
     1141 ggcgacggtg tcgtggatga gatggacttt tggttcagct cgttcggcct gccgatgttc
     1201 ggtttgatcg aagtctgcgt ctttctgttt ttcttcgggc cgtcgcgcgg ctgggacgcg
     1261 ctgcacaccg gttccgtact gcggttgccg cgcttcttca aattcgtcat gaccttcatc
     1321 accccggtct tcctgttcgc gatcctcgcg gggtggctgg tgaccgacgg ctggaaaacg
     1381 atcttgatgc ggaagacggt tgacggccaa ctcgtgccgc tctacacgcc cgaacaggtc
     1441 ccctgggtgc tcgccacgcg tctcttctac ctcgccctcg tcgtcggcac ctgcctgctc
     1501 atccgccgcg cgtgggcgca ccggaattgg gtaaagcatc cgtccgttga accccaaaag
     1561 agccgttgac ctttcaacgc cctttggttt cgggttccag ggttcacgct gcggacggtt
     1621 cggggaaccg tccctagccg ccgcagggcg aatgtcgcgg gtcgcaggtc aacattaaga
     1681 caagccaaga tttccgaagt cagggaatcg gggatgcgtc ccggcgcacc ggcgccggca
     1741 tagacaaaag ggccttgttc gattattata gacgggcttt tcggataacc ccccctcacg
     1801 ggagcacgat gcaagcgaca atcctggacg gaaaagtgtt ggccgcggaa ctgcgggcgg
     1861 agatcgcccg cgagacggcg gaatttaaac ggcaaagcgg cctgacgccg ggactcggcg
     1921 tgattctgat cggagcggat cctgcatccg tctcctatgt caccgccaaa gaaaaggcat
     1981 gtgcggaggc gggcatggtt tcgcaggatg tgcgtgtgcc agccgagacc acgcaggcag
     2041 aggtgctggc cctcgtaaac cgcatgaacg aggattcccg cattcacggc atcttggtcc
     2101 agcttccgct cccccgtcat atcaacgaat cagccgtcct caacgcgatc tccccggaca
     2161 aggatgtgga cggattcacg ccagcgaacg tcggacgcat gatccttggc gaaccctgtt
     2221 atctgccctg caccccgcac ggcatcatcc ggatgctgct gaaagccgag atccaaaccg
     2281 caggccggca tgccgtcgtg gtgggccgca gcaacatcgt gggtaaaccg gtcgcgcatc
     2341 tgctgatgcg caaagccgag ggcggcaacg ccaccgtgac ggtctgccac accggcacgc
     2401 gcgacctcgc ggagcacacg cgccgggccg acattctggt ggtggccgca ggtcggccca
     2461 acaccgtgac cggcgacatg atccggcccg gtgccgtcgt gattgatgtc ggcgtgaacc
     2521 gtatccccga cccctcccgt ccgagcgggt tccgccttgt cggcgatgtg gacttcgatt
     2581 cggcccgccg cgtcgcctcg ctgatcaccc cggttcccgg aggcgtgggg ccgatgacca
     2641 tcaccatgct gctgcataac actctggagg ccgcgaggcg cgcggctgcg cacaccgtac
     2701 ccggtggacc taacccatga aacgtcttgg cgaaccgctc ttcgcgcggg atgagacggg
     2761 ccgtctgaaa acccggatcg gcacgttctt tctgcgtacc cccggactgg tcacgctgcc
     2821 cggcgtgcat gccacgcagc gtatcgcctg gctcaacgaa ctcaaccgtg aacgacaggt
     2881 cgccgggatc cccgccctct cggaagcgga aagcgacgcg gagatcgcgt gttcggtgga
     2941 tttgctgttc aatgaccagc acgtgctcat ccggcccgat ccctacgccg ttgacctggc
     3001 gtttgaagcg gacgaggcgc ttcaggcgct ggtttccaag cgtaagatcc gttttctcaa
     3061 cacgcagaac gagatcatcc gcaatgcgtt gcgcgcgcgc ggcgaaaact ggcgtatgag
     3121 ccgggttccg gtttcgccgg aagagatgaa ccggatgatc gcccaagcgc gggtcgccat
     3181 ttccggtctg ccgctctatt tccacaacgc cgccacgggc acacgctttt tgacgctgga
     3241 aacgtttgcc tggctgggga cgcagccgga cgacgtgttc cgcaagcacc ttgccgaaat
     3301 cgtgcgctac agctcgctgc gtaaccggtt cgggtaccct gaaatcgata tcttccccgt
     3361 aggctgtgcc ttctcccgtc aggcgttcga ggccctgaac gccgaggggc ttgaaatcgg
     3421 agcgctgcgc gccgcctacc ggaagctgct cgcggccttc cgcgaagccg tcccgatcga
     3481 attccacgag gaaacgttcg agaaccttga ttggcgcaac cggctctgct ccacgctcat
     3541 ccatcagccg aacgcggtgg ccacccaaga cctcccggac ggcatggccc ccgaattcca
     3601 tttgcagatc gagtggctac cgggctgccg gattgatgag ggcgaactga tctttgatcc
     3661 ggtctacgaa gagttggacc tgatgccgga ggatgccgaa ctgcgcgcct tttgcgatcc
     3721 gcgggccaga gagatcatct tcaattacgt gcgggagttc accaccatcg aattcatcaa
     3781 cgtcgggcgc atcacgcgct ccctgtcccg tcgcgtgcag accggcgaac accgtaccga
     3841 cgtctacgtt gtgcagatca aggaaaccgg aaaacccaag ccggaacttt gcatccttcg
     3901 ttttcagaaa tggggggtct gcgaacatct ggatgagggc aaagacatgg tccgctcgat
     3961 catggaggcg gtggattaca ccgactacgt gctcgaccgc cgcctcggat gccgccagct
     4021 tggcatgaat ctgcccgaga aagtctcgac cggccggatg ccgaccctgt acaccggctc
     4081 gaactccttg tacaaaggag ccaaatgctg gaccgtgtat ttcgagcgcc agtacatccc
     4141 cggacaagtg accgacaaga tcctgacgcg gcactacgcg aacccggaat tcaacctgct
     4201 tctggcccgg ctgttgggcg aggccgccgt ggtcaactgc gtggtcggcc gggccaatac
     4261 ggagggacag gtcctcttcg atgacggcga cgaagtgatc acgctggatc aggcgggtat
     4321 gccggaacgc ttgaccgtct cggaccatac cggtgccttc accctttacg aagagccgct
     4381 ggagcaaatg gcggaggcct atgcggatcc ggtcaacaag cgaatcgcgc tcatgcccaa
     4441 tccggaggct tttgccgaaa cctacctcgc gtcgttccgg cagcggttcg tgacgttgca
     4501 gcaggagtac cggcggcggc gtcacgcatt cgacaaactg ttcaaacacc ggccggttga
     4561 tccggcaggc agtctcgcct accgctggca atgtattctg gcgcggctcg acgccacaga
     4621 cgccgaaacg ctgacagagg ccattcgcag tcagatccgg ggactgcacc catgaaacag
     4681 tgcctgtgtt tccgggatga gcggttgtgg atccggcgtt cgcccgcagg aaccgaatgg
     4741 ctgttcgaga ccaacccgaa cgcgcagatc ctgcccgtcg aaccgctacc ggacggcacc
     4801 tgggccgctg agtcggagtc tgcacctgtc cccggcgcgg agtccatcgg gttgcgcgaa
     4861 ctgttcgatc tggtgtcagc cgaaacctcg cagaacgcag gcatggctcg gcaactcctg
     4921 cactggcggc ggtcacaccg tttctgcagc gtgtgcgccg cgccgctgga gcgccacccc
     4981 actgaacgcg ccatgtattg cctggcctgc ggacatctcg cttatccccg gatcaacccg
     5041 gttgccatca ccctgattca tcgggggcgc gaggtgctgc tcgcgcggaa agccgggaac
     5101 cagtaccctt tctggagcct gatcgccggt ttcgtggagg tcggggagac tctggaacaa
     5161 accgtccgac gcgaagtgca cgaagaggtg ggtctgcgtg tgcgcgacat ccgctatgcc
     5221 accagccaag cctgggccga ccccagcaac ctgatgatcg gcttcaccgc gtcgtacgat
     5281 ggcggcgaga tccttcccga cggcagggag atcgccgagg ccgactggtt cggatgctcg
     5341 tgtctgccgc cgatcccgtc acgcatcagc atcgcccgcc gcctgatcga tgcttttttc
     5401 acggcatgaa acagggcgca gcctgcggcc gggagcaacg agcgtctgcc cgtccgggca
     5461 agtgcggcgg ggctgacgta ttgagatgcg ggcgaaaccc gcaacctcaa aggcggtgag
     5521 gttgtgagac ggtgaggcgg tgaggctgtg agactgtgag gcggtgagac tg
//
LOCUS       DAOSRB010000176         5570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13306_length_5570_cov_6.967543, whole genome shotgun sequence.
ACCESSION   DAOSRB010000176 DAOSRB010000000
VERSION     DAOSRB010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13306_length_5570_cov_6.967543"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1037)
                     /locus_tag="PLZ74_08955"
     CDS             complement(207..1037)
                     /locus_tag="PLZ74_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOR98489.1"
                     /translation="MGVGKREKGVFAVGAGVLVLAAGIAAFVQYRRVPGASSGGQTVA
                     VPDAAETAAAGSGARATTGKPVRSGSVRGGGSSLANAAQRTREREAKRLASPSPTNEV
                     PLTPEQKLAATMEERLDNGDERGALELARQLLKAESSEVRSDVVTTLGWIGVKALPEL
                     SVMLGDADSEVSEEALQQWIMAIEEIEDPHAKAQMLALAMRSTRDEDTLQSIAMEFNG
                     LPEDVVLRSLQTIIQGQKNGPAVDVAREEYESITGEEYSTPEALEKYILENVEPEDEE
                     "
     gene            complement(1047..2150)
                     /locus_tag="PLZ74_08960"
     CDS             complement(1047..2150)
                     /locus_tag="PLZ74_08960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR98490.1"
                     /translation="MRRTVSRHHGVRRRGAVWSGLLLSGMLLAGCGRNGGRAGIVVGD
                     TEKLISEMTPDEVIVSVNGTPFRRADYDLMLSGLDTLYRANNPSANPMSLEMNRKRRA
                     RTLVREFITKEVLVQEARRRGVKATEAHWKDASRSLAKLAASKNVPVEWFAGAPEAEL
                     RLAYQIARENALILALREAEFGDRLKITEADVEWARQSMAQYNESCDQTNRIVLARGE
                     AICKRIRSGEDFFEVAREVTEDTEVPDGVLGEQVPIEIDDKRVRKAAFSLPVGAVSDP
                     FQTEEGLVIIKVLERKGIDSPLAREQATVNLGRILLLTAHPVAEKSDAELRREIMQTR
                     LKKLQAPWLKALQERTRFEYPNGTNLFRKGKAG"
     gene            complement(2150..4855)
                     /locus_tag="PLZ74_08965"
     CDS             complement(2150..4855)
                     /locus_tag="PLZ74_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOR98491.1"
                     /translation="MKMKLVAGVCAGVACATLFAGAARAAIPTAGAADPVVPGQWHSN
                     LEAATDKAKELNAPMFVVWGEEGCTYCRRLDTILQNETVREYFANSGFILVYEHSLAA
                     AQTPIRRWVGTGQWPLTRLTWWQDGVKVADQKWPRPSTSKNPATVLQVLQNFFEQHVG
                     AFSPVPADTSKDAYDPENDAAEGAAELAWGDQEKTEANLKLASKGGDEPYADSADWYR
                     LPVVSGATYRVWFSDVSGIEGDAPQAAVYGDAGGGSVWAGPAVLASEPEFEFVPTAAG
                     TAYIKVWRETSADTNILYTLHHQRFAPGTIEFVQNAVAVNERAGSVTLQVRRTGGTTG
                     EATVQIGYKDKLTPDSGHTATAGQDFNGAPSPATLTWADGEAAVKTVTFTLIKNVIEW
                     EGDETFGVTLASTTGNEVGPEAVVTLNEADPMVTRAADYTGLVEADADDEDPDERVTG
                     TFEMKVSARGQISGKAIFPRNAPGYAGTFAIRNARHHNINIDTGIATIVGSLVQGRTA
                     VPFELELHMESGLIEGVIGTDEDARSCRMFRADLSARAELLASLQGSYTVAFPVADIV
                     WPLEGAPAGSGYATITVDRRGTFRAAGKLPDGTAYSQSGALFVWPGDGTLGVNGLPRV
                     YAVLFTAPSAYQGGRFSGVLCFGDANANGVWDVTAVEEYPFECVSFKPTSVPVYNLEA
                     PGFDLMLGVAGGRYDRYASLHTVFDGFRFFVDEMKKPGTLLRYTVSTVALNEMTGRSF
                     TARIAKEAECVSWESADPLEVVPNARGRGFIVPRGDLVKTGTDEATGKPLYNYDTGTN
                     PNQFQLRLTSSSGLLNGSFRVHYDYATYINLTREPAVRRWAHKSNMIQYNGILLQEQA
                     VWGAEMTVGYGHYLYSSLAEYPGTARTYRFTQSEEIRLLAIQEGD"
     gene            complement(4971..>5570)
                     /locus_tag="PLZ74_08970"
     CDS             complement(4971..>5570)
                     /locus_tag="PLZ74_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98492.1"
                     /translation="TVPDSKAGYVLPSHCGDPGTNAATCFRKTGKKTIQGFFYVCACE
                     CDSLNSGTTWYLWDKKAKVGAEAGASLEFNVIGKKKSDVEAYVTLTLGDDGVLSADTL
                     YLAGFGKWDAKNCRVKSVSGNFAGQIAPPKCNDALSCDEAAAYICDPDSQTGGCALVA
                     DNTVDTVAFGTWSIKYSSSKAKKLIKDSSAYLDWLPDYF"
BASE COUNT          995 a         1969 c         1489 g         1117 t
ORIGIN      
        1 caacgctctt tttaaggcgt ccgcggacgg ttcggggaac cgtccctgtc gatcgtcgcg
       61 ggtcgacgat cccgataccg ataccgatac cgaccccgac cccgatttgg atgaaaccct
      121 gaaaccctga aaccctgaaa ccccgaaacc cagaaaccct gaaaccctga aaccctgaaa
      181 cctgaaaccc tgaaacccaa gcagggttac tcctcatcct caggctcgac attctccagg
      241 atatattttt cgagcgcttc gggcgtgctg tactcttcac cggtgatgct ttcgtattcc
      301 tcgcgcgcca catcgaccgc agggccgttc ttctggccct gaatgatggt ttgcagactt
      361 cgcaggacca cgtcctcggg aagtccgttg aactccatcg cgatcgattg cagcgtatct
      421 tcatcacgcg tggacctcat ggcgagagcc agcatctgag ccttggcgtg gggatcctcg
      481 atctcttcaa tggccatgat ccactgctgc aacgcctcct cagagacttc gctgtccgca
      541 tcgccaagca tgacggagag ttcgggtaac gccttgactc cgatccaccc caaggtcgtg
      601 accacatccg aacgcacttc gctgctttcg gccttcagca attgacgcgc caattccaac
      661 gcgccccgct cgtccccgtt atccagacgc tcttccatcg tggcggcaag cttctgttcg
      721 ggcgtcagcg gaacctcgtt tgtgggggag ggcgacgcca agcgtttggc ctccctttcg
      781 cgagtccgct gggccgcgtt tgccagcgac gatccccctc cccgaacgct cccgctccgg
      841 acaggttttc ccgttgtcgc ccgcgcaccg ctgcccgctg ccgccgtttc agccgcatcc
      901 ggcacggcaa ccgtttgtcc gccggaagag gccccgggaa cccggcggta ttggacgaac
      961 gccgcgattc ccgccgccaa caccagcacc cccgccccga cagcgaagac ccccttttcc
     1021 cgtttgccta cacccatgaa gcccccctac cccgctttgc ccttgcggaa taaattcgtg
     1081 ccgttcggat actcgaaccg cgtccgttcc tgcaacgcct tcagccaagg ggcttgcaat
     1141 ttcttaagac gcgtctgcat gatttcccgc ctcagttccg cgtcgctctt ttccgcgacg
     1201 ggatgcgccg tcagcagcag aatccgtccc aaattgaccg tggcctgctc cctcgcaagc
     1261 ggagaatcga tccccttgcg ttccagaacc ttgatgatca ccaaaccctc ttccgtctgg
     1321 aacggatcgg agaccgcgcc gaccggcagc gagaacgccg ccttccgaac cctcttgtca
     1381 tcgatttcga tggggacctg ttcccccaaa accccgtcgg ggacctccgt gtcctcggtc
     1441 acctcgcgcg cgacctcaaa aaagtcctcc cccgaacgga tccgtttgca gatcgcctcg
     1501 ccgcgcgcca gaacaatccg gttcgtctgg tcgcacgact cattgtattg cgccatcgac
     1561 tgcctcgccc actcaacatc cgcttcggtg atcttcagcc ggtccccgaa ctccgcttcg
     1621 cgaagcgcca gaatcagcgc gttctcgcgc gcaatctgat aagcgagacg cagttcggct
     1681 tccggtgccc cggcgaacca ttcgaccggc acattcttgc ttgcggcgag cttggccaag
     1741 cttctgctgg catctttcca atgggcttcg gtcgccttca ccccgcgtcg gcgcgcctcc
     1801 tgaaccaaaa cctctttggt gatgaactcc cgcaccagag tcctggcgcg ccgtttccgg
     1861 ttcatctcca aggacatcgg attggcggag gggttgttgg cgcggtacag cgtgtccagc
     1921 ccggacaaca tcaggtcata atccgcgcgc cggaacgggg tgccgttcac ggacacgatg
     1981 acctcatccg gcgtcatctc ggagatgagt ttttccgtat ccccgaccac gattccggca
     2041 cggcctccgt ttcgtccgca tccggccagc agcattccgg acagaaggag tcccgaccac
     2101 accgctcccc gcctcctcac cccatggtgc cgcgagacgg ttctcctcat caatcaccct
     2161 cctggatcgc cagcaagcgg atttcctccg actgcgtaaa cctgtaggtc cgcgccgttc
     2221 cgggatattc cgccaggctc gaatacagat aatgtccgta tcccacggtc atttccgcgc
     2281 cccaaacagc ctgctcttgc agcagaatcc cgttgtactg gatcatgttg ctcttatgag
     2341 cccaccgcct gaccgccggt tcccgcgtga gattgatgta ggtcgcgtaa tcgtaatgca
     2401 cgcggaacga accgttcagc agtccgctcg atgatgtcag gcgcaactgg aactggttcg
     2461 gattggtccc cgtgtcgtaa ttgtagagcg gttttccggt cgcttcgtcg gttccggtct
     2521 tgaccaggtc cccgcgcggg acgatgaacc ctctccctct cgcattcggc accacttcaa
     2581 gcggatcggc ggactcccac gaaacacact ccgcctcttt ggcgattcgc gccgtgaatg
     2641 aacgtccggt catctcgtta agcgccaccg tgctcactgt gtagcgcaac agggtgcccg
     2701 gctttttcat ctcgtcgacg aaaaagcgga acccgtcgaa aaccgtgtgc agcgaggcgt
     2761 accggtcata tcttcctccg gccaccccga gcatcagatc gaaaccgggg gcttccagat
     2821 tgtacaccgg aacggacgtc ggcttgaaac tcacgcattc gaacgggtac tcctccacgg
     2881 ccgtgacgtc ccaaacgccg tttgcgttcg cgtcgccgaa gcagagcaca cccgagaagc
     2941 gtccgccctg atacgcagag ggcgcggtga acagcaccgc ataaacccgc ggcagtccgt
     3001 tcacgcccaa cgtcccgtcc cccggccaaa cgaacagtgc gccgctctgg gaatacgccg
     3061 tgccgtccgg gagtttgccg gcggcgcgga aggttccgcg cctgtccacg gtgatcgtcg
     3121 cgtagcccga tccggcgggc gcgccctcca gcggccacac gatatccgcc accgggaagg
     3181 ccacggtata cgagccttgc aggctggcga gcagctcggc gcgcgcgctg agatcggccc
     3241 ggaacatccg gcacgaacgc gcgtcctcat ccgtgccgat caccccttcg ataagcccgc
     3301 tctccatgtg cagttccagt tcgaacggaa ccgcggtccg gccctgcacc agcgagccga
     3361 cgatcgtcgc gatgccggtg tcgatattga tgttgtggtg ccgcgcgttg cggatcgcga
     3421 acgtccccgc gtaacccggc gcgttccgcg ggaaaatcgc cttgccggag atctggcccc
     3481 tggcggacac cttcatctcg aacgtgcctg tgacgcgctc gtccggatcc tcgtcatccg
     3541 catccgcctc caccagtccg gtatagtctg ccgcgcgcgt caccatgggg tcggcctcgt
     3601 taagcgtcac caccgcttcc ggcccgacct cgttccccgt cgttgatgcc agggtcacgc
     3661 cgaacgtttc atcaccctcc cactcgatca cgttcttgat cagcgtaaac gtcacggtct
     3721 tcacggcagc ctcgccgtcc gcccacgtca gcgtggccgg cgaaggcgcg ccgttgaaat
     3781 cctgccccgc cgtggccgta tgcccggaat cgggggtcag cttgtcctta tacccgatct
     3841 gcaccgtcgc ctcgccggtt gtgccgcccg tgcgccggac ctggagcgtc acgctgcccg
     3901 ctctctcatt caccgccaca gcgttctgca cgaattcgat cgtgcccggc gcgaagcgct
     3961 ggtgatggag cgtgtaaagg atattggtgt ccgcggaagt ctcgcgccag accttgatat
     4021 acgcggttcc cgcggctgtc ggcacaaact cgaattccgg ctcactcgcc agcacggcgg
     4081 gcccggccca caccgaccct ccgccggcat cgccatacac cgcggcctgc ggcgcatccc
     4141 cctcgatccc gctcacatcc gagaaccata cccggtaggt cgcgccgctc accaccggaa
     4201 gccgatacca gtccgcggaa tccgcatagg gctcatcgcc gcctttgctc gcgagcttga
     4261 ggttggcctc ggtcttttcc tggtcgcccc aggccagttc cgccgcgccc tcagccgcgt
     4321 cgttctccgg gtcataggcg tctttggagg tgtccgccgg caccggcgag aaggccccga
     4381 catgctgctc gaaaaagttc tgcagcacct gcagcaccgt ggccggattc ttactggtgc
     4441 tgggtcgcgg ccacttctga tctgcgacct tcacgccgtc ctgccaccac gtcaggcgcg
     4501 tcagcggcca ttgccctgtt ccgacccacc gcctgatggg ggtttgggca gcggccaagg
     4561 aatgttcgta caccagaata aacccgctgt tcgcgaaata ctccctcacg gtctcattct
     4621 gtaagatcgt gtcaagcctg cggcaatacg tgcagccctc ttcgccccac accacaaaca
     4681 tcggcgcgtt cagttccttg gccttgtctg tcgcggcctc aagattgctg tgccactgcc
     4741 ccggcaccac cggatcggca gcccctgcgg tcgggatcgc tgcccgcgcc gcccccgcga
     4801 aaagagtcgc gcacgccact ccggcacata caccagcaac taacttcatc ttcatttgtg
     4861 gtctcctaat gatccgtcca tggatagcgt accgaatcca tgggccgcat gcaaataaaa
     4921 aaagggcggg gtttcccccc gcccttcact gaatccgtca gtttcccgac ttagaagtag
     4981 tcaggcagcc aatcaaggta ggcgcttgag tctttaatca gcttcttcgc cttcgaggag
     5041 ctgtacttga tcgaccaggt tccgaacgca accgtatcga ccgtgttatc agcaaccagc
     5101 gcacaaccac ctgtttggct gtcggggtcg cagatatacg ccgcggcctc atcgcaggac
     5161 agcgcgtcgt tgcacttggg cggcgcgatt tgaccggcga agtttccgct cacgctcttc
     5221 acccggcagt tcttggcatc ccacttgccg aaaccggcaa gatagagcgt gtcggcactc
     5281 aacaccccgt cgtcaccaag ggtgagggtc acataagcct caacatcgct cttcttcttg
     5341 ccaatgacgt tgaactcaag cgatgcgccc gcttccgctc ccaccttcgc cttcttgtcc
     5401 cagaggtacc aagtcgtgcc ggaattgagg gaatcgcact cgcatgcgca aacgtagaag
     5461 aatccctgga tggtcttctt cccggtcttg cggaagcaag tcgccgcgtt cgtcccaggg
     5521 tcgccacagt gcgagggcag gacgtaaccc gccttcgagt cgggcaccgt
//
LOCUS       DAOSRB010000177         5540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13390_length_5540_cov_6.273838, whole genome shotgun sequence.
ACCESSION   DAOSRB010000177 DAOSRB010000000
VERSION     DAOSRB010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13390_length_5540_cov_6.273838"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1416
                     /locus_tag="PLZ74_08975"
     CDS             25..1416
                     /locus_tag="PLZ74_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98493.1"
                     /translation="MLIETVVLQVDLGDDLQTGMDWVQRGKQWQYVDDTSKPIYETHP
                     DTGVKTRIGYEQMRVRTRGTIFNNGDQLGGGGGGDDGNTKGLFGSLLGLGTAVLSNGV
                     ASGYLGAPNGISYFLKSDKLNIAALIEASKSDSRTKVLSSPVLMTVDNKEATLEATTM
                     RYLYKGVRYSGGSIDNREVPDFEQRDIGLTVKITPRINPNGAVVLTIEEKFETVGADQ
                     NVGGAKYPTVNTRKLQADVAVENMQTVVLGGLVQNEIKTSDFGIPILKDIPYIGKYLF
                     GKTSFSDNRSELLVFLTPYVLDTPQDVEAEAARRKAALSDDQAWVDYGWSKSKLADPM
                     SSSEKVRRTKQRWATEDANHKAEKEYERLLDAREEQLRERARKDAEDVLKRAKQDAEK
                     TAEGSDDDDDTEAKLRDLLEKLKAKKARESTAAAPVPDATESVIRVTDSVEILGKGAG
                     SPPQAATPETPKP"
     gene            complement(1514..2737)
                     /locus_tag="PLZ74_08980"
     CDS             complement(1514..2737)
                     /locus_tag="PLZ74_08980"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOR98494.1"
                     /translation="MIRVNEHYAKLQSSYLFSEIAKRVKAYQAEHPDKRILRLGIGDV
                     TEPLPPACVAALHQAVDEMASRSSFRGYGPEQGYDFLREAIARNDFQARGAEITPQEI
                     FVSDGAKCDTANIQELFTDKLTLAIPDPVYPVYVDTNVMAGRTGKCVKGRYKGLVYLD
                     STESNGFVPDLPEQPVDLIYLCFPNNPTGATATREQLAAWVRYARENRALILFDAAYE
                     AFIRDPALPHSIYEIEGARDVAIEFRSFSKTAGFTGTRCAYTVVPTSTMAYTKKGEPV
                     ALNPMWNRRHTTKFNGVAYPVQRAAEAVYSAEGQAQTRALTDFYLGNAARIRAAFTEL
                     GYSLTGGENSPYIWVNVGKDSWKFFDKLLKRAQVVTTPGAGFGRCGQGYIRISAFNSR
                     ENVEEAMMRLRKVLK"
     gene            complement(2734..3684)
                     /locus_tag="PLZ74_08985"
     CDS             complement(2734..3684)
                     /locus_tag="PLZ74_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR98495.1"
                     /translation="MPIRLQHRIEYAALRAAETILNALPYRVALGTAWLAAFFLFTVL
                     RFRRQETFRRIREVFGGDYPSRAVRHIAWRSLRNMTFNVVELMRAPAVDRAWIDRHLP
                     TFATYVPEVQEQIRQYGGAVITVPHMGNWDLAGWACHHYGISMFSIAGKQKNPLVNAW
                     INRRRECGMTILERGGGTLRQIRTLLREGSVLAILPDVRVYTPDLEVAFLGGKANVGR
                     GMARFAMAAGVPVIPAILRREGWTRHGFDLLPSILPDPSREREAEARRITETVLAHVD
                     AAIRRTPDQWFWYNKRWVLAPVGKPSRPQAATSGTERENP"
     gene            complement(3805..5187)
                     /gene="pcnB"
                     /locus_tag="PLZ74_08990"
     CDS             complement(3805..5187)
                     /gene="pcnB"
                     /locus_tag="PLZ74_08990"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOR98496.1"
                     /translation="MFFQKAYPIVRERKDHCLSRANIAPEALKVLYRLIRAGYTAYLV
                     GGSVRDLLMGRQPKDFDISTNARPNEIRRLFRNCFLIGRRFRLAHIVFGHKIIETSTF
                     RRQPDSNDGDGGSGIYQTEDNTFGSPEEDAKRRDFTVNGLFYDPQSFSVIDYVGGIRD
                     LEKRVLRSIGDPDIRFREDPVRMMRAVKFAARLDFSLDRACRKAILKHHADILNASVP
                     RLCEEVFRLFTYGTSRTAFRLCWEYKLLGDLMPELDAFISRNGGANASLWNVLAALDA
                     DPQSADLTNGVRVACLYYPLFRAGLEQEEARYPRKRANRQHVARRILQPLAHRLHIPK
                     ATLYSAITLLDAGRRFAEPPQKGRSLRFLHHPAFPEALAFYRIISAAEKTDRSRLDAW
                     QALYDAEVAQRKANGSRDAESDPPARARRRRRRKPRTDRAATAPTQPSRHPVTVTEVP
                     SAKADTRTDS"
BASE COUNT         1111 a         1515 c         1832 g         1082 t
ORIGIN      
        1 ggcatggaca tccagctctc gcaggtgctg atcgagaccg tggtgctgca ggtcgatctg
       61 ggcgatgacc tgcaaaccgg catggactgg gtgcagcgcg gcaagcaatg gcaatatgtg
      121 gatgatacat ccaagccaat ttacgaaacg catcccgata ccggagttaa aacacgaata
      181 ggttatgagc aaatgcgcgt tagaactcga ggtactatct tcaacaacgg cgaccagttg
      241 ggcggcggcg gcggcggtga cgacggaaac acaaaaggcc tgtttgggag tttattaggt
      301 ctcggcacgg ccgtgctaag caacggtgtt gcttccggct atttgggtgc tccaaatgga
      361 atcagttact ttctcaaatc cgacaagctg aacatcgcgg ccttgattga ggcatcgaag
      421 tcggactcgc ggaccaaggt gctctcctcg ccggtgctga tgacggtgga caataaggaa
      481 gcgacgctgg aggcgaccac catgcgttat ctctacaaag gggtaagata ctctggagga
      541 tcaatcgata acagagaggt gccggacttc gaacagcggg acattggtct gacagtcaaa
      601 atcacgccgc gcatcaaccc gaacggggcg gtggtgctga cgattgagga aaaatttgaa
      661 acggtgggtg cggatcagaa tgttggggga gcaaagtatc ctaccgtcaa tacgcgcaaa
      721 cttcaggcgg atgtcgcggt ggagaacatg cagaccgtgg tgttgggcgg actggttcag
      781 aatgaaatca agacatcgga tttcgggata cccatactca aggacatccc ctacatcggc
      841 aagtatctgt tcgggaagac ctcgttttca gacaaccgtt ccgaactcct ggtctttctg
      901 accccctacg tgttggacac gccgcaagat gtcgaggccg aggccgcacg tagaaaggcg
      961 gctctgtccg atgatcaggc ctgggtggat tacggctggt cgaagagcaa gttggctgat
     1021 cccatgtcat cgtcggagaa ggtgaggcgc accaagcagc gctgggccac ggaagacgcc
     1081 aatcataagg ctgagaaaga atacgaaagg ctgctggatg cccgcgagga gcagttgcgg
     1141 gagcgcgcga ggaaagatgc ggaggatgtg ctgaagcgcg cgaagcagga cgcggaaaag
     1201 acggcggaag gttcggatga cgacgacgat acggaagcga aacttcgcga tctgctggaa
     1261 aagctgaagg caaaaaaagc gcgcgaaagt acggccgccg caccggttcc ggatgcgacc
     1321 gagtcggtga tccgcgtcac ggacagcgtg gagatcctcg gaaagggggc gggcagcccg
     1381 ccccaagccg ccacgccgga aacgccgaag ccgtgaggct gggttacgag accttcggga
     1441 gtttgtggaa acccgttcgg acgaggtcgt ggccaatgcg gctggaaaca cccggcgcca
     1501 cccattcccc gggtcacttc agcaccttgc gcagccgcat catcgcctct tccacatttt
     1561 cacggctgtt gaaggcgctg atgcggatgt atccctggcc gcagcggccg aaacccgcac
     1621 cgggcgtggt caccacttgc gcgcgcttca gcagtttgtc gaaaaacttc caggagtcct
     1681 tgccgacgtt gacccagata tacggactgt tctcgccgcc ggtcagagag tatccgagct
     1741 cggtaaaggc ggcgcggatc cgcgcggcgt tgccgagata gaaatcggtc agcgcgcggg
     1801 tttgagcttg gccctcggca gagtaaaccg cctcggcggc acgttgcacc ggataggcca
     1861 cgccgttgaa tttggtggtg tgacgccggt tccacatcgg gttcagcgcg acgggctcgc
     1921 cttttttggt gtaggccatc gttgacgtcg gcaccaccgt atacgcgcag cgcgtgccgg
     1981 taaagcctgc ggttttggaa aaactgcgga attcgatggc cacgtcccgc gcgccctcga
     2041 tctcgtaaat actgtggggc agggccgggt cgcggataaa cgcctcgtag gcggcatcga
     2101 acagaatcaa cgcccggttt tcgcgggcat agcgcaccca ggccgcgagt tgttcgcggg
     2161 tggcggtcgc gccggtcgga ttgttcggga aacagaggta gatgaggtcc accggctgct
     2221 cgggcaggtc agggacaaag ccgttcgatt ctgtgctgtc cagatatacc agccctttat
     2281 agcggccttt gacgcatttg ccggtgcgtc ccgccatgac gttggtgtcc acatagaccg
     2341 ggtagaccgg gtcggggatc gcgagggtca gcttgtccgt gaaaagctct tggatgttgg
     2401 cggtgtcgca cttcgccccg tcgctcacaa agatctcctg tggggtgatc tccgcgccgc
     2461 gcgcctgaaa atcgttgcgg gcgatcgcct cgcgcaggaa gtcgtatccc tgttccggtc
     2521 cgtatccccg gaacgaggag cgcgacgcca tctcgtccac ggcctgatgc agggctgcca
     2581 cacaggcggg gggcagcggt tcggtcacgt cgccgatgcc gaggcgcagg atccgcttgt
     2641 ccggatgctc ggcctgatac gctttgacgc gcttggcgat ctcggaaaaa aggtaggagg
     2701 attggagttt ggcgtagtgc tcattgacgc ggatcatggg ttttcccttt cggttccgga
     2761 tgtcgcggcc tgcggtcggg acggtttgcc gaccggggcg agcacccagc gtttgttata
     2821 ccaaaaccac tggtcgggtg tccgccggat ggcggcgtcg acgtgggcaa gaacggtttc
     2881 cgtgatgcgg cgtgcctcgg cctcgcgttc ccgcgaaggg tctggcagaa tggagggcag
     2941 cagatcgaac ccgtggcggg tccaaccttc gcgccgtagg atggcgggga tcaccgggac
     3001 tcctgcggcc atggcgaaac gcgccatgcc gcgtcccaca ttcgccttgc cgccgagaaa
     3061 ggcgacttcg agatcaggcg tgtagacgcg gacatccggc aggatcgcca acacgctgcc
     3121 ctcgcgcagc aaggtccgga tctggcgcag tgtgccgccg ccgcgttcga ggatcgtcat
     3181 gccgcactcc cggcgccggt tgatccatgc gttgaccaac gggtttttct gctttccggc
     3241 gatgctgaac atgctgatgc cgtagtggtg gcaggcccat cccgccagat cccagttgcc
     3301 catgtgcggt accgtgatga ccgcgccgcc gtattgccgg atctgttcct gaacttccgg
     3361 gacgtacgtc gcgaaggtcg gcaggtggcg gtcgatccac gcgcggtcca ctgcgggcgc
     3421 gcgcatcagt tccacgacat tgaacgtcat gttgcggagg gatcgccagg cgatgtgccg
     3481 gaccgcgcgg gaaggatagt ccccgccgaa cacctcgcgg atgcggcgga aggtttcctg
     3541 tcggcggaac cggaggacgg tgaagagaaa gaaggccgcg agccaagccg tgcccagcgc
     3601 gacccgatag ggcagcgcgt tcaggatcgt ctccgccgcc cgcagggcgg cgtattcaat
     3661 gcggtgctgg agacggatgg gcatgtcggg tggttgtcta gccagaatta caggattaac
     3721 aggatttgga ttaagcgggg tacatcatgc aaatcgtgtt aatccgtaat cttggctaaa
     3781 aaatgatctc tttcgttttg ctggctaact gtcggtacgc gtgtccgcct ttgctgacgg
     3841 cacctccgtg acggtcacgg ggtgacggga tggttgcgtc ggcgcggtgg cggcacgatc
     3901 ggtacggggc ttgcgacggc gccgtcgccg cgcgcgcgca ggaggatcgc tctccgcatc
     3961 gcgggatccg ttcgctttgc gctgcgcgac ttccgcatcg tagagggcct gccaggcgtc
     4021 cagccgggaa cggtcggtct tttcggcggc cgagatgatg cggtagaacg cgagcgcctc
     4081 cggaaaggcg gggtggtgga gaaaacgcag ggagcgtcct ttttgcgggg gttccgcaaa
     4141 acggcgtccg gcgtccaaga gcgtgatggc cgaatagagg gtcgctttgg ggatatgcag
     4201 gcggtgcgcg agcggttgca ggatccgacg cgccacatgc tgacggttgg cccgtttgcg
     4261 ggggtaccgc gcctcctcct gctcgaggcc cgcccggaag agcgggtaat acaggcaggc
     4321 tacccggacg ccgttggtca gatccgcgct ctgcggatcg gcatccagcg cggcgagcac
     4381 attccacagc gatgcgttgg cgccgccgtt ccgggagatg aaggcatcga gttccggcat
     4441 cagatcgccc aacagtttgt attcccagca gagacggaat gcggtgcgcg aagtgccgta
     4501 ggtgaagagc cggaacacct cctcgcacaa gcgggggacc gaggcgttga gaatgtccgc
     4561 atggtgtttg agtatggctt tgcggcaggc gcggtccagc gagaaatcca ggcgcgcggc
     4621 gaacttgacg gcgcgcatca tgcggaccgg atcctcgcgg aaccggatat ccgggtcgcc
     4681 gatactgcgc agcacccgtt tttcgaggtc ccggatcccc ccgacgtaat cgatcacgga
     4741 aaaggactgg gggtcgtaga acaaaccgtt cacggtgaaa tcacggcgct tggcatcttc
     4801 ctctggcgag ccgaaggtgt tgtcttccgt ctggtagatg cccgagcccc cgtccccgtc
     4861 gttcgagtcc ggctggcgcc ggaaggtgga ggtttcgata attttatggc cgaaaacgat
     4921 atgggccaaa cggaaacggc gtccgatcag gaaacagttg cggaagagcc gccggatttc
     4981 gttcggacgc gcattcgtgc tgatgtcaaa atccttgggt tgtcttccca tcaggaggtc
     5041 gcgcacactg ccgcccacca gataggcggt gtatcccgca cggatcaggc ggtagaggac
     5101 tttaagagct tcgggggcaa tattcgcgcg ggagaggcaa tgatctttgc gttcgcgcac
     5161 gatcgggtaa gctttttgaa aaaacatggt ttttagtcat tcaccggttg caaaccgaat
     5221 cgtcataaca gaattaacga agtatgccaa aaaaccggcg tcgaaacaag cggcggaatg
     5281 cacgttgcgc ggttgaacct tcaactctct ttggtttcaa ggtttcaggg tttctggttt
     5341 catggtttca gggtttcatc caaatcgggg tcgggatcgc tatcgctatc gggatcgatt
     5401 gtttaaccac gaacacacgg gatggcatcg ctcgggtttt tggggaacgc caaggggagt
     5461 ccctgatttt catgaagttt catgtgtttc agggtttcat ccaaatcggg gtcgggatcg
     5521 ctatcgctat cgggatcgat
//
LOCUS       DAOSRB010000178         5525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13448_length_5525_cov_6.079525, whole genome shotgun sequence.
ACCESSION   DAOSRB010000178 DAOSRB010000000
VERSION     DAOSRB010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13448_length_5525_cov_6.079525"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /gene="panB"
                     /locus_tag="PLZ74_08995"
     CDS             <1..740
                     /gene="panB"
                     /locus_tag="PLZ74_08995"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOR98497.1"
                     /translation="SARLADAAGLPLLLVGDSLGMTVLGYESTLPVTLEQMLHHTAAV
                     ARAARNAMVVADMPFLSYQVCEAEAIRNAGRFLQEAGADAVKLEGGAFRAGLVSALTQ
                     NGIPVLGHIGLTPQSVNQLGGYKVQGKSREAGERLIADARALAEAGAFAIVLECTPPD
                     IAATITAAVPVPIIGVGAGPVCDGQMLVMHDLLGLSEGRPPRFVKAYAALAETMTQAF
                     RTYAEEVRDGVYPAHEHCYAPRKPGST"
     gene            760..2178
                     /locus_tag="PLZ74_09000"
     CDS             760..2178
                     /locus_tag="PLZ74_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HOR98498.1"
                     /translation="MPPSRTSREDIVITGMACRFAESPSLAAFWRNLLQRQSLLTPLP
                     VPSPTEPAPPRRTLFDRPYPTLCGQLGDLYACTPADQYFPRKMNAGENQDVFFTVQLA
                     IDALRDSGSTLNSLPTDRVSLHLGYAPPFNAASVNWLQHTFFIDQTLGIVQNFFPGVG
                     AEQIEEVRKQLTASLPEPNPYAFLSALGCVIASWTAHYLGFAGPAFLVDAGAVSGHQT
                     LQGAMDDLITRRSDIALAGAVQPPFSDAALQGFAGAIPFSRGKTLQPFSREADGTLPG
                     EGGAMFVLKRLKDALRQNDRIYAIIRSIGIAAAAMDQHHRVPTPERLTRAISRALHAA
                     DITPESVQLIEAHGSAIPHSDQTELKVLHDLYGDRRRGQALTGVGSVKGNIGHTLWSA
                     GAAGILKAALAIAHRTLVPTVSVDKPHLSLASAKSPLYLLSDARPWLRGNRKNPRRAC
                     VSAIDFTGTCAAAILEEYPEDP"
     gene            2175..5471
                     /locus_tag="PLZ74_09005"
     CDS             2175..5471
                     /locus_tag="PLZ74_09005"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOR98499.1"
                     /translation="MSSAPHLYDAFDSELILIGASNDDDLALTVRRVLGFLEHAPDVP
                     LQDVAYTCAVSARHMPSILAVVASAVPDLRDRLSLALRKLEARANRIRDKSGTYFFRE
                     RLRPSGRIAFLFPGAVSFYPDMLRDLCLAFDACRGAFDELQEALQTQDPECITPADYL
                     FPPSACYRNDTGVFQTDGFSESFIAAHAANTAFYRLIDSMGVTPDGLTGFSGGDFAAL
                     DVAGVYGNLSRNKRIVFLREGYQMLNRLAEREDLPTCIMLSVLDASRERIDELIAAHP
                     GRMALTCFHSPRQLTVALSPEILKEVTASLQASGAKTMPVPMNRPFNTPWCSKVLPPI
                     EQFLSHWVRHTPRIPVYSCATAERLPAQPRSILKLTADQWTSPILFDDTIRQMYQDGF
                     RLFIELGARGNMTTAVDETLKNLPHQAVAINRIHRSGLVQLHHALGQLAAQGVQLDLT
                     VLHRHRRQHRLDFDRPLALAVTTEHTMRLSSQLPRLTVFAPRGDLLTAAAPPAGTTRK
                     SRAPRETGRHLDFGADFPMLTNAEILEEQAGILELAKVLTLEDYPFLRDYAIGTSQLS
                     YANPELKGLTLLSMISGLEMMTETARKLVPRRRVAQIDNLRATRWVGFERGAVRIILR
                     AERIAWPDPQFSAVRVQLRDGAPNSAFTWPIIEATVLLTAAPAVMPPIHPPPLTRARP
                     VNWSGHDIYPDRLFHGDCLRIVRHVDLWSEEGIDFEIEVPERSQAVRVTRIPLFSIWP
                     MLLDGIVSSFSLWRSHERFAGAISLPFRARRILFYAPGFTEGARLRGYLRLVSVTPRS
                     HVADIQISDGNGNLLIHFRGWEELCERVPPEYQQFILHPSEKFLTQPLPLELLGNPTT
                     PVAGAVATDIPFSLFEHNQELWLKTLAFVLLSPTEREEWLEMQGATSRRVEWLFGRAA
                     AKESARRFLRQFHQARWTAADIPIWPDDSGKPHLLGPWREHTAVTLDLSIAHTSKLIV
                     AAVAANARLGIDIEAIGRDLSEDFTKGVFTNEELERAAQTGEAPTAILRFWCAKEALS
                     KALGTGIRYSPRDLLIRAIDPVTGQIEIELLGQWLEPFKQLKGRRNLVSTSLYEGHVF
                     ASCLLPASLF"
BASE COUNT          935 a         2006 c         1556 g         1028 t
ORIGIN      
        1 ccagcgcgcg ccttgccgat gccgccggcc ttcccctgtt gctggtcggc gactcgctcg
       61 gcatgacggt tctcggttac gagtccaccc tgcccgtcac gctggaacag atgctccacc
      121 acaccgctgc cgtggcgcgg gccgcgcgca acgcgatggt ggtggcggac atgcctttcc
      181 tctcttatca ggtctgcgag gccgaggcga tccgcaatgc cggccgtttt ctgcaggagg
      241 ctggtgcaga tgccgtcaaa ctcgaaggcg gcgcgttccg tgccgggctg gttagcgcgc
      301 tgacgcagaa cgggatcccc gtgctcgggc acatcggcct cacgccgcag tcggtcaacc
      361 aactcggcgg ctacaaagtt cagggcaaat cgcgtgaggc gggcgaacgg ttgatcgcgg
      421 acgcccgggc gttggcggag gcgggcgcct tcgccatcgt gctcgagtgc accccgcccg
      481 atatcgccgc caccatcacc gcagccgttc ccgtcccgat catcggggtc ggggcggggc
      541 cggtctgtga cggccagatg ctggtaatgc acgatctgct cggcctttcc gaaggccggc
      601 cgccccgttt cgtcaaggcc tatgccgctt tggcggagac catgacgcag gctttccgca
      661 cctacgccga ggaagtccgt gacggagttt atccggcaca cgaacactgt tacgcgcccc
      721 ggaaacccgg atcgacctaa cccgccccac cctttttcta tgcccccttc ccgcacatca
      781 cgcgaagata tcgtcatcac cggcatggcg tgccggttcg ccgaatcgcc gtcacttgcc
      841 gcgttctggc gcaacctgct ccagcgccag tcgttgctga ccccgcttcc cgtcccatcc
      901 cccaccgaac ccgcgccgcc ccgtcgcact ctgttcgacc gcccctaccc cacactctgc
      961 ggccagttgg gtgacctgta cgcctgtacg cccgcagacc agtatttccc ccgtaaaatg
     1021 aacgcgggcg aaaaccagga cgtgtttttc accgttcaac tcgcgatcga cgccctgcgc
     1081 gacagcggca gcaccctcaa cagcttgcca acggaccggg tctcgctgca cctcgggtac
     1141 gccccgccct tcaatgccgc ctccgtcaac tggctgcagc acaccttctt catcgaccag
     1201 actctcggga tcgtgcagaa tttcttcccg ggcgtcggcg cggagcagat tgaggaagta
     1261 cgcaaacagc taaccgcctc gctgcccgaa cccaacccgt acgcctttct ctcggccctc
     1321 ggttgtgtca tcgcctcctg gaccgcccat tatctcggat tcgccgggcc tgcctttctg
     1381 gtggatgcag gcgctgtttc gggtcaccag accctgcagg gcgccatgga tgacctgatc
     1441 acgcgccgtt cggacatcgc gcttgcgggt gcggtgcagc cgccgttcag cgacgccgcc
     1501 ctgcaggggt tcgcgggcgc gatcccgttc tcccgcggca aaacactcca gccgttcagc
     1561 cgcgaagccg acggcaccct gcccggcgag ggcggcgcga tgtttgtcct caaacgcctc
     1621 aaggacgccc tgcgccagaa tgaccgcatc tacgccatca tccgctccat cggcatcgcg
     1681 gctgccgcca tggatcagca tcatcgcgtc cccacgccgg aacggcttac acgcgccatc
     1741 agccgtgccc tgcatgcggc ggacatcact ccggaatcgg ttcaactgat cgaagcccac
     1801 ggatccgcaa tcccgcactc ggaccagacc gaactcaagg tgttgcatga cctctacggt
     1861 gatcggcggc gcggccaggc cctgaccggt gtggggtccg tcaaaggcaa tatcggtcac
     1921 accctgtggt ccgcgggcgc ggcgggcatc ctcaaggccg ccttggccat cgcgcaccgc
     1981 actctggtgc cgaccgtatc cgtagacaaa ccccaccttt ccctcgcttc cgccaaatcc
     2041 cccctctatc tgctctccga tgcgcgtccg tggctgcggg gcaaccgcaa gaacccgcgt
     2101 cgtgcctgcg tctcggcgat cgacttcacc ggcacatgcg cggccgcaat cctcgaagaa
     2161 tacccggagg acccatgagc agtgcccctc atctgtatga cgctttcgac agcgaactga
     2221 tcctgatcgg tgcgtccaac gacgatgatc tcgcgctgac cgtccggcgt gtgctcggtt
     2281 ttctggagca cgcgccggac gtgccgctgc aggatgtcgc ctacacttgc gccgtttccg
     2341 cccgccacat gccctccatt cttgcggtgg tcgcctccgc cgtgcccgac ctgcgcgacc
     2401 gcctgtcgct ggccctgcgg aaactggagg ctcgcgccaa tcggatccgc gacaagagcg
     2461 gcacctattt cttccgtgag cgcctgcgcc ccagcggccg gatcgccttc ctctttcccg
     2521 gcgcggtctc gttctatccg gatatgctgc gcgatctgtg cctcgccttc gacgcctgcc
     2581 gaggcgcctt cgacgagttg caggaagccc tgcaaaccca ggaccccgaa tgcatcaccc
     2641 cggccgatta cctgttcccg ccctcggcct gttaccggaa cgacaccggc gtcttccaaa
     2701 ccgacggatt ctcggagtcc tttatcgccg cgcacgccgc gaataccgct ttttaccgtc
     2761 tcatcgacag catgggggtc acgcccgacg gattgacagg gttcagcggc ggcgatttcg
     2821 cggctctgga tgtggccggc gtctacggca acctctcccg caacaagcgt atcgtcttcc
     2881 ttcgggaagg ctatcagatg ttgaaccggc tggcggagcg cgaagacctg cccacctgca
     2941 tcatgctctc cgtgctggac gcctcgcgcg aacggattga cgaactgatc gcagcacatc
     3001 ccggccgaat ggcactgacc tgtttccaca gtccgcgcca gttgaccgtg gccctctcgc
     3061 ccgaaatcct caaagaggtc accgcttccc tgcaagcatc tggcgcgaag accatgcccg
     3121 ttccgatgaa ccggccgttc aacacgccgt ggtgttcgaa agtcctcccg cccatcgagc
     3181 agttcctttc gcattgggtg cggcacacgc cgcgcatccc ggtttattca tgcgccaccg
     3241 ctgagcgtct gcccgcccaa ccgcggagca tcctgaaact caccgccgat caatggacct
     3301 ccccgattct gtttgacgac acgatccggc agatgtatca ggacggcttc cgcctgttca
     3361 tcgaactcgg cgcgcgcgga aacatgacca ccgctgtcga cgaaacactg aaaaacctgc
     3421 cccatcaggc tgtcgccatc aaccgcatcc accgttcggg gctggtccag cttcaccatg
     3481 ccctcgggca actcgccgcc caaggggttc agctcgacct caccgtgctt caccgccacc
     3541 gtcggcagca ccggctggat ttcgataggc cgctcgccct cgccgtcacc acagagcaca
     3601 cgatgcgcct ctcctcgcaa ttgccgcgcc tgaccgtttt cgccccacgc ggcgacctcc
     3661 tcaccgcagc cgcccctccg gccggcacca cgcgtaaatc ccgcgccccc cgcgaaaccg
     3721 gacgccatct cgatttcggc gccgacttcc cgatgctgac caatgcggag attctcgaag
     3781 aacaggcggg catcctagaa ttagcgaagg tcctaaccct cgaagactat ccgttcctga
     3841 gggactatgc gatcggcacc tcgcaactct cctacgcgaa tccggaactg aaggggctca
     3901 ccctgttgtc catgatctcg ggcctggaaa tgatgaccga gaccgcgcgc aaactcgtgc
     3961 cgcgtcggcg cgtcgcacag atcgacaatt tacgcgcgac gcgctgggtc ggctttgagc
     4021 gcggtgccgt ccggatcatt ctccgcgccg agcggatcgc ctggcccgac ccgcagttct
     4081 ccgcggtccg tgtccagctc cgcgatggcg cgcccaacag cgccttcacc tggccgatca
     4141 ttgaggccac cgtgctgttg actgccgcac ctgcggtcat gccgcccatc caccccccgc
     4201 ccctcacccg ggcgcgtccg gtgaactggt cggggcacga catctatccc gaccgtcttt
     4261 tccacggcga ctgtttgcgc attgtccgcc acgtcgacct ctggagcgag gagggcattg
     4321 atttcgagat cgaagtgccg gaacgcagtc aggcggtgcg tgtcacccgc atcccgctct
     4381 tttcgatctg gcccatgttg ctggacggga tcgtctcgag tttctcactc tggcgttcgc
     4441 atgaaaggtt cgccggcgcg atctcgctgc cgttccgcgc tcgccgcatc ctcttctatg
     4501 cccccggctt cacggagggc gcgcgcctgc gcgggtatct gcggctggtc tcggtcaccc
     4561 cgcgctcgca tgtggcggac atccagatct ccgacggcaa cggcaacctg ctcatccact
     4621 tccgcggctg ggaggagttg tgcgagcggg tcccgcccga gtaccagcag ttcatcctgc
     4681 acccctcgga aaagttcctc acgcaaccgc tgccgttgga actgctgggc aatccgacca
     4741 caccggtcgc cggtgcggtc gcgaccgata tccccttcag cctgttcgaa cacaatcagg
     4801 agctctggct caaaaccctg gcctttgtgc tgttgtcccc cacggaacgt gaagagtggc
     4861 tggaaatgca aggcgccacc agcaggcggg tcgaatggct cttcgggcgc gccgccgcaa
     4921 aagagtcggc gcgccgcttc ctgcggcaat ttcatcaggc gcgctggacc gcggcggaca
     4981 tcccgatttg gcccgacgac tccggcaagc cgcacctgct cggcccctgg cgcgagcata
     5041 cggccgtaac cctcgacctc tccatcgccc atacctctaa gcttattgtt gcggccgtag
     5101 ctgccaacgc gcggctaggt atcgacattg aagccatcgg acgggacctg agcgaggatt
     5161 tcaccaaagg cgtgttcacc aacgaggagt tggaacgggc cgcacagacc ggcgaggccc
     5221 caactgccat cctgcgcttt tggtgcgcga aagaggccct ctcgaaagcg ctcggaaccg
     5281 gcatccgcta cagcccgcgg gatctgctga tccgcgccat cgaccccgtc accggccaga
     5341 tcgaaatcga actcctcgga caatggctgg aaccgttcaa acagttgaaa ggccgcagga
     5401 atctcgtcag cacatcgctc tacgaggggc atgtcttcgc ctcctgcctg ctgccggcct
     5461 cgctgttctg agaaccgtaa acctataatc ctaaaaagag cacaggtttc tggtttcagg
     5521 gtttc
//
LOCUS       DAOSRB010000179         5497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13533_length_5497_cov_5.102903, whole genome shotgun sequence.
ACCESSION   DAOSRB010000179 DAOSRB010000000
VERSION     DAOSRB010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13533_length_5497_cov_5.102903"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /locus_tag="PLZ74_09010"
     CDS             complement(<1..1890)
                     /locus_tag="PLZ74_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98500.1"
                     /translation="MMKKAIVCGTMMVVCTCGVFGGNIEVANIPDTAVLTQVGEYDLL
                     TFTNNGSFTVTSPGEVDVLVVAGGGGGGSHSGGGGGGGGVFYITNHLVSCGDYTVCVG
                     TGGVSDVNGGDSSIFGWTAVGGGKGGGVAAAGGNGGSGGGGGAGNGGVHNIKRAFPGG
                     TGTDGQGYNGGASTNNNTASSFTSPAVGGGGGGGGGPGSDATVTLVISEDFATTTTVY
                     KCGNGGNGFACAITGLEKYYGGGGGGGTGNGNSSVAGSGGLGGGGAGAYSTRTGGAGE
                     NLTGGGGGGGGRNANTGYPGGPGGSGVVMLRWKNTSRADVSTGKVTAWGGTAVWDDGY
                     ISRSFVLDGQLTFSEETEVELLMVAGGGGGSNGSRGGGGGAGGVIYTNALMVPAGTYN
                     IIVGRGGGAGTNGENSVALGFTAVGGGHGGSANVGGAAGGSGGGGGARNNGTTSPGGA
                     CTEGQGNIGGTGYNNGGAGGGGGIGSPGGNVTSGNRINSGAGGTGMLYRVSGHAFYYA
                     GGGNGTQNRTWVPGGGGRGISQTIGEDAEPFSGGGGGGTGQAMNQGWAAGVAGIGGSG
                     QVVVRYREAEFGYPDLTREAQGGEISSYSENGHRYRVHTFTENGTFTMPAWGRVSLLV
                     VGGGGG"
     gene            2132..3001
                     /gene="speB"
                     /locus_tag="PLZ74_09015"
     CDS             2132..3001
                     /gene="speB"
                     /locus_tag="PLZ74_09015"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOR98501.1"
                     /translation="MKNAFLASECPPVPADDAAFHVIPAPMELSVSYGQGTRLGPSAI
                     LAASQQLEACDAEGRLTPARIHTQRAVSSRSRDPEVWIAAVEKRVAQTLACRSVPVLL
                     GGEHTVSLGAARAFHAARDLVGFIQFDAHADLRGTYEGSPLSHACVMRRIHELGFPCL
                     QIATRAVSPEEQEYRKAHRSSISFYDARVIADDGMSRHFIPRGFPKRVYITFDLDGLD
                     PAVLPATGTPVPGGLFWYDAIRILRRIAATRDIVGLDVVELAPNRASPHADFTAALLT
                     QTLMGFAAASPTR"
     gene            3017..4828
                     /locus_tag="PLZ74_09020"
     CDS             3017..4828
                     /locus_tag="PLZ74_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR98502.1"
                     /translation="MALISLRKVSVQYGGPAVLDQISLSIEPGERACVTGRNGEGKST
                     LLKLLAGLIVPDTGERIIQPDLRVAYLSQDVPGDLDGTVAEIAGSGVVDNGACHAHHP
                     GAAMYLSQLKLDGAEQFNTLSGGLRRRALLARALAAEPHLLLLDEPTNHLDIETIEWI
                     ETFLQRSPCACLFVTHDRAFLKRVATRVLDLDRGRLIGWDCDYDTFLRRKQEQLDDEA
                     VYWSRQTRKLEQEEAWLRRGVKARTTRNEGRVAALLQLRETFRQRRTAPGVSRFQLDL
                     ETSSGEQVLKVSDVSFAYPGGAPVVCGFSAKILRGERIGIIGPNGSGKTTLLNLLCGH
                     LTPDSGYVQPGARVEIALFDQLRSALDLDRTVQQNLAEDREEVMVGCVRKHVFGYLSD
                     FLFTPDRARTPVRALSGGERARLMLAKLFLQPGNLLVMDEPTNDLDMETLELLEEQLM
                     NYDGTLLLVSHDRTFLDNVVTGTFVLDGSGKVELYPGGYSDWLRQRPAPSTLPAVEPD
                     EASVALPARTPPSERRRERLTFKQQRDRQMLPQQIESLETEIASLHAVLSDPEHYRRE
                     PDATREATGRLAQAEAELEAAFKQWVELETLAERLEK"
     gene            4959..>5497
                     /locus_tag="PLZ74_09025"
     CDS             4959..>5497
                     /locus_tag="PLZ74_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOR98503.1"
                     /translation="MNRHPDTLCVQEGWQPKNGEPRVLPIIQSTTFKYDSSEHVGDLF
                     DLKVPGHFYTRLSNPTTEAVENKLAALEGGVGALMTAAGQSASLIAILNLCNSGDHFI
                     ASSAIYGGTTNLFSVTLKKLGIEVTYVDSHATDEEIRAAFRPTTRAVFGETIANPALT
                     VFDIERFAHVAHANGVPLIV"
BASE COUNT          971 a         1945 c         1551 g         1030 t
ORIGIN      
        1 gccgccgccg ccgcccacga cgagcagtga gacacggccc cacgcgggca ttgtgaacgt
       61 gccgttctcg gtgaatgtgt ggacacgata ccggtgcccg ttttccgaat acgacgaaat
      121 ctcaccgccc tgcgcctccc gcgtcaggtc gggataaccg aactcagcct cgcggtagcg
      181 gacgacgacc tgaccggaac cgccgattcc agcgactccg gccgcccaac cctggttcat
      241 cgcctgtccc gtccctcctc cccctcctcc agagaacggt tcggcgtctt caccgatcgt
      301 ttgggaaatc ccgcgtccgc cgcctcccgg cacccatgtg cggttctgcg tgccgttgcc
      361 gcctcccgcg tagtaaaagg catggccgga aacacggtat agcatgcccg ttccacccgc
      421 gccgctgttg atcctgtttc cgcttgtgac attgcctccc gggcttccta tgccgccacc
      481 gccgcccgca ccgccgttgt tgtatcccgt gccgccgata ttcccttgtc cctccgtaca
      541 cgcaccgccc ggggatgtcg tgccgttgtt gcgcgcgccg ccgccccctc cggaaccgcc
      601 ggctgcaccg ccaacgttcg cactgccgcc gtggccgccg ccgaccgccg tgaaccccaa
      661 cgcgaccgag ttttcaccgt tggtgcctgc tccgccacct ctgccgacga tgatgttata
      721 ggtgcctgca ggaaccatca gcgcgttcgt gtaaataacg ccgcccgcgc ctcctccgcc
      781 gccgcgcgaa ccgttcgatc ctcctccgcc gcctgcgacc atgaggagtt ccacttccgt
      841 ttcctccgag aacgtgagct gtccatctaa aacgaacgac ctcgagatgt atccgtcgtc
      901 ccacaccgca gtaccgcccc aggccgtcac cttccccgtc gacacatcgg cgcgcgaggt
      961 gttcttccaa cgcaacatca cgacccccga accgcccgga ccgccagggt atcccgtgtt
     1021 ggcgttccgt ccgccgccgc ctcctccgcc gccggtcagg ttctcacccg ctccgccggt
     1081 tctcgtggaa tatgcgccgg caccgccgcc gcccaaaccg ccggacccgg cgacggaaga
     1141 attgccgtta ccggttccgc cgccgccgcc gccgccataa tatttttcga ggccggttat
     1201 tgcacaggcg aacccgttgc cgccgttccc gcacttgtag acagtggtcg tcgtcgcgaa
     1261 gtcctcgctg atgacgaggg taacggtagc gtcgctgccc ggaccgccgc cgccaccccc
     1321 gccgccaccg accgcagggc tggtaaacga tgacgctgtg ttgttgttcg tgcttgcgcc
     1381 gccgttgtat ccctgcccgt cagtgccggt tccgcctgga aatgctcttt tgatgttgtg
     1441 gacgcccccg ttaccggcgc cgccgccacc gccggaaccg ccgttgccac cggccgccgc
     1501 gacacccccg cctttaccgc cgccgaccgc cgtccacccg aaaatcgagg agtcgccgcc
     1561 gttgacgtca cttacaccgc ctgtacccac acacacggtg tagtcgccgc aagaaaccag
     1621 atgattcgtt atgtaaaaaa cgccccctcc gccaccaccg ccgcccgaat gcgaaccgcc
     1681 gccaccgccc cccgcgacca cgaggacatc cacctcgccc gggctcgtca ccgtaaacga
     1741 cccgttgttc gtgaacgtga ggaggtcgta ttcaccaacc tgcgtcagca cggccgtatc
     1801 cgggatattc gcaacttcga tgttgccgcc gaacacaccg cacgtacata caaccatcat
     1861 tgttccgcac acaattgctt tcttcatcat gcgcctctct ggtattcctt tgatgctttc
     1921 ggtgacatgc cgaagcacaa aacatcgtga taaagaatac tcgcacattc cgaggcgttt
     1981 gtctatcccg ccctttttga aaaatcgttt catggtattg ttttgcaaca tgttatacat
     2041 taaaattact ggctgttgac gtccggggct tgcatcccgt tccgtacccc ggtatgcttc
     2101 cgtttttcgt attccccacc cttctcacat tatgaagaat gcttttctcg cttccgaatg
     2161 tcccccggtc ccggccgatg acgccgcctt tcatgtgatc cccgcaccga tggaactcag
     2221 cgtctcgtac gggcagggca cccgactggg cccgtccgca attttggcag cctcgcaaca
     2281 actcgaagcc tgcgacgccg aaggacgcct cacccccgcg cgtatccata cacagcgtgc
     2341 ggtgtcctcg cgcagccgcg acccggaggt ctggatcgcg gcggttgaga agcgggtggc
     2401 gcagaccctc gcctgccggt ccgttccggt tctgctcggg ggcgagcaca cggtgtcgct
     2461 cggcgcggcg cgcgctttcc acgcggcgcg cgatctcgtc gggttcatcc agttcgacgc
     2521 gcacgccgac ctgcgcggca catatgaggg ttcgccgctg tcgcatgcct gcgtgatgcg
     2581 gcgcattcac gagttggggt tcccctgcct gcagatcgcc acccgcgcgg tctcacccga
     2641 agagcaggag taccggaagg cccatcgttc ctcgatctcg ttttatgacg cgcgtgtcat
     2701 cgcggacgac ggcatgagcc gtcacttcat tccgcgcggt tttccgaaac gggtttatat
     2761 cacctttgat ctggacgggc ttgaccccgc cgtgctgcca gccaccggca caccggtacc
     2821 cggcgggctt ttctggtatg acgcgatccg catcctgcgc cgcatcgctg ccacgcgtga
     2881 tatcgtgggt ctggatgtgg tcgaactcgc accgaaccgc gcgagcccgc atgctgattt
     2941 cactgccgcg ctgttgacgc aaaccctgat ggggttcgcc gcggcatccc ccacgcgtta
     3001 accccggaac cgctcgatgg ccctgatttc cctcagaaag gtgagtgtcc aatacggcgg
     3061 accggccgta ttggaccaga tcagcctcag catcgaaccg ggtgaacgcg cctgtgtcac
     3121 cggtcgtaac ggcgaaggca aatcgaccct gctgaagctg ctcgcgggcc tgatcgtccc
     3181 ggacactggg gaaagaatca tccagcccga cctgcgggtg gcctacctct cccaagatgt
     3241 gccgggcgat ctggacggca cggtggccga aatcgccggg tctggagtcg tcgacaacgg
     3301 ggcttgccat gcccatcatc ccggtgcggc gatgtacctt tcccaactga agttggacgg
     3361 cgcggagcaa ttcaacacgc tctcgggcgg cctgcgacgg cgcgcactgc tcgcacgggc
     3421 actcgctgcg gagccgcacc tgctattact cgacgagcca accaaccatc tcgacatcga
     3481 gacgatcgag tggatagaaa ccttcttaca gcgcagcccc tgcgcctgcc tcttcgtgac
     3541 gcatgaccgc gccttcctga agcgcgtcgc cacgcgggtg ttggatctcg accgcggccg
     3601 actgatagga tgggattgcg attacgacac tttcctgcgg cggaaacagg aacagctcga
     3661 tgacgaggcc gtgtactggt cgcgccagac ccgtaaactc gaacaggagg aggcttggct
     3721 ccggcgcggg gtcaaagccc gcacaacccg caatgaggga cgcgtggccg cactgctcca
     3781 gttacgcgaa accttccgtc agcgccgtac cgcaccaggc gtcagccggt tccagcttga
     3841 tctggagacc tcttcaggcg aacaggtgct caaggtttca gatgtctcct tcgcctatcc
     3901 cggaggggcg ccggtggtct gcgggttctc cgccaagatt ctccgcggcg aacggatcgg
     3961 gatcatcggc cccaacggat cggggaaaac caccctgctc aacctgctgt gcggtcacct
     4021 cactccggat tctggctacg tccaacccgg cgcgcgggtg gaaatcgccc tgttcgacca
     4081 gctccgcagc gctctcgatc tcgaccggac cgtccagcag aatctggcgg aagaccgcga
     4141 ggaggtgatg gtcggctgtg tgcgtaaaca cgtgttcggt tacctcagtg attttctctt
     4201 caccccggat cgggcgcgga cgcccgtgcg ggcgctctcc ggcggggaac gcgcccggct
     4261 gatgctggcc aaactttttc tgcaacccgg caacctgctg gtcatggacg agccgaccaa
     4321 cgatctggac atggagacgc tggagttgct ggaagagcag ttgatgaatt atgacggcac
     4381 gctcctgttg gtcagccatg atcggacttt tctcgataat gtggtcacgg gcaccttcgt
     4441 gctggacggg agcggcaagg tcgaactcta tcccggcgga tactccgatt ggctgcgcca
     4501 acgtcccgcg ccgtcaaccc tcccggccgt ggaaccggac gaggcgtcgg tcgcgctccc
     4561 ggcacgcaca ccgccctcgg aacgccgacg cgaacgcctc acctttaaac agcagcgcga
     4621 tcggcagatg ctcccccaac agatcgagtc gctggaaacc gagatcgcct cgctgcacgc
     4681 cgtcctgagc gacccggaac actaccggcg ggagccggac gcgacccgcg aggccaccgg
     4741 ccgcctcgcg caggccgagg ctgaactgga agccgcattc aaacagtggg tggaactcga
     4801 aacccttgcc gaacggttgg aaaaatgaaa ccgtgatcgc cgcacgccat ccttgtccgg
     4861 ggtgggccgc tgtgttatca tttgctgaat gattgccgaa tgtttcggcc ctctgacccc
     4921 ggcccatttg accggtgccg cgatgacaag gagaagcaat gaaccgacac cccgacaccc
     4981 tttgcgtgca ggagggctgg cagccgaaaa acggcgaacc gcgtgtgctg cccatcattc
     5041 agagcacgac cttcaagtac gacagcagcg agcacgtcgg cgacctcttt gacctgaagg
     5101 tgcccggcca cttctacacc cggctcagca atcccacaac cgaggcggtc gagaacaagc
     5161 tcgccgcgct ggaaggcggc gtcggcgcgc tgatgaccgc ggcgggccag tccgcatcgc
     5221 tgatcgccat tctcaacctc tgcaacagcg gtgaccactt catcgcctcg agtgccatct
     5281 acggcggcac caccaacctc ttcagcgtca cactcaagaa actgggtatc gaggtcacct
     5341 atgtagattc ccatgccacc gacgaggaga tccgggccgc cttccgtccc accacccgcg
     5401 cggtcttcgg cgagactatc gccaaccccg cgctgacggt cttcgacatc gagcgctttg
     5461 cgcacgtggc gcatgccaac ggggtgcccc tgatcgt
//
LOCUS       DAOSRB010000180         5458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13642_length_5458_cov_8.411438, whole genome shotgun sequence.
ACCESSION   DAOSRB010000180 DAOSRB010000000
VERSION     DAOSRB010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13642_length_5458_cov_8.411438"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLZ74_09030"
     CDS             complement(<1..643)
                     /locus_tag="PLZ74_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOR98504.1"
                     /translation="MSVEGLKEDYAWHMRYTLAKEMENASPFDQYTAFAYCVRDRIIE
                     RWIETIACHRNQNVKRVYYLSLEFLIGRLLGNNVINLKMDEICREALADYGIDWHALR
                     DIEVDAGLGNGGLGRLAACFLDSMSTLNLPCVGYGLRYDYGIFKQRIVDGQQVEEPDH
                     WLKEGSPWEVARPENTQIVNFGGRVECITNGRIEWRWVETEQVIGIPYDLPIVG"
     gene            1165..4131
                     /locus_tag="PLZ74_09035"
     CDS             1165..4131
                     /locus_tag="PLZ74_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOR98505.1"
                     /translation="MKQLRVLTCAVGLALMAAMSFAGGYRLKYIATGWDIGRATPEQI
                     LKMMPKFEQTAFDGVAIQCSAPQDDALLDAGYIFNDPAWRYESFEKLVETYRAINSRG
                     KLKENFISAGLSARNTERNLAKHGGRVRLDDDAAWARIAHNYGMLAKIAKAGGFRGIQ
                     LDNEDYHGLHQFEFRPGDGDYEAAAEKMRQRGREVCGAIFREFPDVRLGFFWAFSSVR
                     RQVQAPDPLKAIRESNRLFPQFLNGLLDVMPPEAVVIDFDEDAYTFRGADGTFERTFV
                     HMMKHALVAVAPENRAKYRAQFRNSFLLYLDQYVGSKYRVRAGKRGQPNNWYRAPVNG
                     SRAGAFYADLEAAANAADGYLGIYGERFGFVDWEHELDAAMKPWNALLSRITWDEELG
                     LDAKLRLVRDPHAFMLEELAAAKKDPARWNLLETLPNFGHARPGTFIVHEEVTNRNYF
                     AVTFESKGETAAVARWQNGLAWNWEPGGVRHVAFTKEGVSGDGWSLYTALLRVPVAAT
                     RLQLQIGTNEVRNAAMYRLDPPPTRPFVLAYQLDISRGKVPTLPNLKRIVDTIASLGY
                     TQFQLYMECAFAYKGYEAAWKDRSPMTPDEMRELSTYCRSKGMDLVPSQNTFAHMGPW
                     FEVADLRKRLAECPDGANIEAPKFKKKRGPVTLVASGGESLKFLSDLFDQLLPCAESR
                     YVNIGCDEVYDLWDKNCRSAAKVAKDGYARTYFDHVLAVRKLAYQRQRQSMFWADAIF
                     EYPELIREIPKDMIALVYGYTAGGGDKFDEKCAALAADEVRFYVCPGTSGWCGPSGRT
                     KNMLGNVKEACEAGRRHGAEGLMVCDWGDGGHHQPWIVALPSLVYAAGLVQGKDLTRA
                     EIAAKVDAICGVAVGAALIRYGDVYETTPHLRYLYHCYSTPQSEKIGPESLAVVREAK
                     SLAELEKAPTWVKEDFATIDFLYGLIEAHAQGKVPDAKSLEEDYRTLWLKQNRPGGFE
                     NSLRFLKEKPHETQRR"
     gene            4151..4972
                     /locus_tag="PLZ74_09040"
     CDS             4151..4972
                     /locus_tag="PLZ74_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOR98506.1"
                     /translation="MTKMKWLTGIAMMLACGAVADAECNGTVPPKRMTIVEELFAPHS
                     VILFQGDSITHGGRLRDMNHYLGHGYQAEIAMRYLAYRPDLRLQFGNRGVSGHTSSNL
                     VARWHHDAVPYTPDESGYGGVFPGERNKPRVPDVLSILIGINDYLRKSDGWFVPVADY
                     ERNLRFMVTNSLAANPKMKIVLCEPFRLPEDTNPDFLARQATVRRVATDYNLVFVPFQ
                     KLFSEELLKEEPRPRYWFWDAFHPTYAAHMRMADFWIATVAREFASGKGNPSLRR"
BASE COUNT         1081 a         1553 c         1709 g         1115 t
ORIGIN      
        1 atccgacgat cggaagatcg tacgggattc cgatgacctg ttcggtctcg acccagcgcc
       61 actcgatccg gccgttggtg atgcactcga cccggccgcc aaagttgacg atctgggtgt
      121 tttccggccg cgccacttcc cagggcgaac cttctttgag ccagtgatcc ggctcctcga
      181 cctgctggcc gtccacaatc cgctgtttga agatgccgta gtcataacgc agcccgtatc
      241 ccacgcaggg caggttgagc gtcgacatgg agtccagaaa acaggcggcg agtcggccga
      301 gaccgccgtt gcccaacccg gcgtcgacct caatgtcgcg cagggcgtgc cagtcgatgc
      361 cgtaatccgc aagcgcctcc cggcagatct cgtccatctt caggttgatg acgttgttgc
      421 cgagcagacg cccgatcaga aattcgaggg agaggtaata gacgcgcttg acgttttgat
      481 tgcggtggca ggcgatggtt tcgatccagc gttcgatgat acggtcgcgc acacagtagg
      541 caaaggccgt gtactggtca aacggcgaag cgttttccat ctccttggcg agggtataac
      601 gcatatgcca ggcatagtct tccttgagcc cttcgacgct catttcaaca cggtggtcac
      661 gttgcgattt gcgaggggga gtcttttgag ccatagtgtc cttgtaatcc ttttgatcct
      721 taaaacgttt ccaatagtat gacagaaatc cggaagggcg tcacgttata aggtcagcaa
      781 ggcatacggg gtttgggcgt atcctcgttc ccgtgaccgt tgaaattttg gtttcaggcg
      841 cctggtccag acgggagcgc gggcgtcccc gcccgcaaca acgcgccgtg cgccacaaag
      901 cgggcaagat gcccgcgctc ccggctgcgc atcaaaggat tcgatgatgg ctgctgatcc
      961 tgtcccgctc cttgggcgac accttggcga tcggaatcga aagttcagcg cagccaacgt
     1021 caacatacac gcggacgggg acgaggacga gtcgtgcgca ttgactgatg gtgggcagac
     1081 ggtttgactc tttcggtccg gttcggcaat aatgatcgcg gcgtggaata atactgcagg
     1141 gtaatcattt cgggacgagg ggaaatgaaa cagctcagag tgttgacgtg tgctgtgggg
     1201 cttgcgctga tggcagcgat gtcctttgcg ggcggataca ggctcaagta catcgccacc
     1261 ggctgggaca tcgggcgggc gacgcccgag cagatactga agatgatgcc gaagttcgaa
     1321 cagacggcct tcgacggtgt ggcgatccag tgttcggcgc cgcaggacga cgcgttgctg
     1381 gatgcgggct acatcttcaa cgatcccgct tggcgctacg agtcgttcga gaagttggtc
     1441 gagacgtatc gggcgatcaa ctcccgcggc aaactgaagg aaaacttcat ctctgcggga
     1501 ctctccgcgc gcaacaccga acgcaatctt gcgaagcacg gcggccgcgt ccgtctcgac
     1561 gacgacgcag cctgggcgcg tatcgcccac aactacggca tgctcgcgaa gattgcaaag
     1621 gcgggcggct tccgcggcat ccaactcgac aacgaggatt accacggact tcatcagttc
     1681 gagtttcgcc ccggtgacgg ggattacgaa gctgcggccg agaagatgcg gcagcgcgga
     1741 cgggaggtgt gcggtgcgat cttcagggag ttcccagacg tccggctcgg tttcttctgg
     1801 gcgttttcga gcgtccgtcg gcaggtgcag gcacctgatc ctttgaaggc gatccgcgaa
     1861 tcgaaccggc ttttcccgca gttcttgaac ggactcctcg atgttatgcc gcccgaggcg
     1921 gtggtgatcg actttgacga ggacgcctac accttccgtg gcgcggacgg aacattcgag
     1981 aggacgttcg tccacatgat gaaacatgcg cttgtcgccg tcgcgccgga gaatcgtgcg
     2041 aaataccgtg cgcagttccg caactcgttc cttctctacc tcgaccagta cgtgggctcc
     2101 aagtaccgcg tccgcgcggg caagcgcggt cagccgaaca actggtaccg cgcgcccgta
     2161 aacggttcgc gcgccggggc gttctacgcc gatctcgaag cggccgcaaa cgccgcggac
     2221 ggatatttgg gcatctacgg cgagcgtttc ggctttgtcg actgggagca cgagctggac
     2281 gccgcgatga agccgtggaa cgctctcctc tcgcgcatca catgggatga ggaactcggg
     2341 ctcgatgcga aactccggct cgtccgcgac cctcatgcgt ttatgctcga agaactcgct
     2401 gcggcgaaga aggatcctgc gcggtggaat ctccttgaga cgctgcctaa cttcgggcat
     2461 gcgcgtcccg gcacgttcat cgtccatgag gaggtgacaa accgcaacta cttcgcggtg
     2521 acttttgagt caaagggcga gaccgctgcg gtcgcgcgct ggcagaacgg actggcctgg
     2581 aactgggaac cggggggcgt ccggcacgtc gcgtttacaa aagagggcgt ttccggggac
     2641 ggctggtcgc tctacacggc gcttctgcgc gttcctgtcg cggcgacgcg tctccagttg
     2701 cagatcggga cgaacgaggt ccgtaacgcc gcgatgtacc ggcttgatcc accgccgacg
     2761 cgtcccttcg tgctcgctta ccaactcgac atctcgcgcg gcaaagtccc gacgttgccg
     2821 aatctgaagc gcatcgtcga cacaatcgcc tcactcggct acacgcagtt tcagctctat
     2881 atggagtgcg cgttcgcata caaaggctac gaggcggcgt ggaaggaccg cagccccatg
     2941 acgcctgacg agatgcgcga gctttcaacg tactgccggt cgaagggcat ggatctcgtt
     3001 cccagccaga acacattcgc gcacatgggg ccgtggttcg aggtcgccga cttgcgcaag
     3061 cgccttgcgg agtgtccgga cggcgcgaat atcgaggcgc cgaagttcaa gaagaagcgc
     3121 gggccggtca cgctcgtcgc ctccggcggc gaatccctca agtttctcag cgaccttttc
     3181 gaccagctcc tgccgtgcgc cgagagtcgc tacgtgaaca tcggctgcga cgaggtatac
     3241 gacctctggg ataaaaactg ccgcagtgcg gcaaaggtcg cgaaagacgg ttatgcgcgt
     3301 acctatttcg accacgtgct cgccgttcgg aagctcgcct accagcgcca gcgccaatcc
     3361 atgttctggg cagacgcgat tttcgagtat cccgagttga tccgcgagat ccccaaggac
     3421 atgatcgcgc ttgtctacgg ctacacggcg ggcggcggcg acaagttcga tgagaagtgc
     3481 gcggcgctcg cggctgacga ggtccgcttc tacgtctgtc cggggacgag cggctggtgt
     3541 ggtccctcgg gccgcacgaa gaacatgttg ggcaatgtga aggaggcgtg cgaggcgggg
     3601 cggcgtcatg gtgcggaagg cctgatggtc tgcgactggg gcgacggcgg acaccatcag
     3661 ccgtggattg tggcgcttcc gtcgcttgtc tatgcggcgg gccttgtgca gggcaaggac
     3721 ctgacgcgcg ccgaaatcgc cgcgaaggtc gatgcgatct gcggggttgc ggtcggggcg
     3781 gcgctcatac ggtacgggga cgtctacgag acaacaccgc acctgcggta tctttaccac
     3841 tgctacagca ctccgcagtc cgagaagatc gggccggaat ctcttgccgt cgttcgggag
     3901 gcgaagtcac ttgccgaact cgaaaaagcg ccgacttggg tgaaagagga tttcgcgacc
     3961 atcgacttcc tctacggcct cattgaagcg catgcgcagg gcaaggtgcc cgatgcgaag
     4021 tctctggaag aggattatcg cacgctttgg ctcaagcaga accggccggg cggttttgag
     4081 aattcgctca gattcctcaa ggaaaaacca cacgaaactc agaggcgctg attagaagga
     4141 gtatgcagac atgacgaaga tgaagtggtt gactggcatc gcgatgatgt tggcgtgcgg
     4201 tgcggtggct gacgcggaat gcaacggcac ggtcccgcct aagcggatga cgattgtcga
     4261 ggagctgttc gcaccccatt cggtgattct tttccaaggt gattccatta cgcacggcgg
     4321 gcgcttgcgc gatatgaacc actatctggg tcacggttat caggcggaaa tcgccatgcg
     4381 ctatttagcc taccgccccg atctgcgtct tcagttcggt aatcgcggcg tcagcgggca
     4441 cacgagttcc aatcttgtgg cgcgctggca tcatgacgcg gttccgtaca cgccggacga
     4501 aagcggttat ggcggtgtgt tcccgggcga gcggaacaag ccgcgtgtgc cggacgtact
     4561 gtcgatcctg atcggcatca acgactacct gcgcaagagc gacggctggt ttgtgcctgt
     4621 tgccgactac gaacgaaatt tgcgcttcat ggtgacgaat tctctcgcgg caaatccgaa
     4681 aatgaagatc gtgttgtgcg aaccgtttcg tctgccagag gacaccaatc ctgattttct
     4741 tgcgcgccaa gcgaccgtgc ggcgcgtggc gacggactac aatctggtgt ttgtgccgtt
     4801 tcaaaagctg ttctcagaag agctgctcaa ggaagaaccg cggccgcgct actggttctg
     4861 ggatgcgttt catcccacgt acgcggcaca catgcggatg gcggacttct ggattgcgac
     4921 cgtggcacgc gagtttgcct cgggcaaggg caaccccagt ttacgtcggt agcgcctgga
     4981 cagtttggaa gcctctttgg agttccggac gtcacattac ctgtccccgc tgtgtcgtcc
     5041 ccactgtgtc ccctgacgcc cgtcgtgatg taacacgcgc ttgtttggac gtcacggaca
     5101 caccgcccgg tgtgttgaaa acgaatcgat cagtccgcat gagtatctcg tcacccgcat
     5161 cgaacagaca aaggtcatcg aaatgctgga ttatgtcaac cgcttgtacg cggttggtga
     5221 cagggcgggt catgcgaaga caatgcgttt ctggtgaaag ttgggatgcg ttttttggac
     5281 atgatttccc gctgcgcggg cgcataaacg acaagattga catgattttt ccgacaaagg
     5341 gacaggtatt tagacactta tcagctttgt tccgaactga atatcccgac ttcccggctt
     5401 ctacaggttg tggggatgag tgacagtacc ctgcggcggc ggcgagtatc cggacggt
//
LOCUS       DAOSRB010000181         5453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13659_length_5453_cov_7.377362, whole genome shotgun sequence.
ACCESSION   DAOSRB010000181 DAOSRB010000000
VERSION     DAOSRB010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13659_length_5453_cov_7.377362"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1068)
                     /locus_tag="PLZ74_09045"
     CDS             complement(244..1068)
                     /locus_tag="PLZ74_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98507.1"
                     /translation="MGRRWLGVAVYGAVLVTFTSNVHAEEFDVHVNVRAYFESAYLSS
                     GGSLTYTRPVAEQYASLTVQSRNYGRLSSSAWICSALNDQTDHKHRRAFYIYEGTLLY
                     GYDVKLAETLTVSTDGGILWDWYGGYEEKPRTPIAWYARQYWHNPWVTPYWIGLGCVT
                     PTTNGRMCFGLRKPFKPADSFTVTPYAETTWGDVNRFRSNYGKGPDHHFLGGSFMFAV
                     MGVLTEWRFTESCYLWGRYRHYVLYNKQARELINARDVPMAKTTYPIFGLGLGCRF"
     gene            complement(1177..1494)
                     /locus_tag="PLZ74_09050"
     CDS             complement(1177..1494)
                     /locus_tag="PLZ74_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98508.1"
                     /translation="MACDSAALLACSLFAETRVWTSGNGNRSNPANWQGNAKPMSGNV
                     VIIRNPGDTPITVNNDTAGLSMQAVYFDGAHFALEFCLFNCRLHSAAGWCMVSRLVPK
                     SRG"
     gene            complement(1547..2404)
                     /locus_tag="PLZ74_09055"
     CDS             complement(1547..2404)
                     /locus_tag="PLZ74_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98509.1"
                     /translation="MNSRTVLQGCLILFLLGLTVRAGEKLPVIETHPHDLDCLIESGH
                     IQGACCSEKGFYLSHQLGIDSFDWNGVHKKHITVPAHLGDIAYADGKIFGAFALRGKE
                     IRKKYGSKGLIRVWNEDLDPLYERAVDDSLDGALVVGDTLYAGPDRFGKVAHSNCCIR
                     TFDLNLNPKEEKTLQLGYSVVYGVQTMGTDGKEIILGHYGGSSRVTFDLKMIGTLRKH
                     SGLCLSEGFGLVPKSVSRREIPVFFTVNAMGGNMHGWRADPKKNPPRIQIRFYEYSEG
                     LFKDITDYR"
     gene            2803..5295
                     /locus_tag="PLZ74_09060"
     CDS             2803..5295
                     /locus_tag="PLZ74_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98510.1"
                     /translation="MATVTVQASAFENILHNPELRRDKSGYAFDWNFTYEGLEDSLRF
                     GDEDGIGTATLLPSKLSTCGVRQVDIRLKCGGKYKIGALVRTKNCTCTQGGIIIYNRA
                     WVKSEGVRPFPRDTDGKWVRLEKEITAPESRGGMYVFTVYASNLTEGEISFREPYLIA
                     LDAESDAEVVRPPKLSELRAITPITPLLGKLPEGDATVTFSFLDEIEKDVCKVALKTE
                     GDGDFCEIGRFPIIGGLIKAKLPDLKPGRGSIRVAVCDKSGVEVAANEYPVRVLARAE
                     TPPEGRRLNELVTRILTAESKDGEFRFQAPRDGWIWISLERGNEGTTVTLDDGTEPVI
                     IHRENERYETMRHVKSGEHVLSVKGATGGSLVVNSIPMIAGFTVPIRFFKDYYEVFGG
                     KFCRERIFPVINTFSIGYPLRVIGEDELADLKSRGKELLGQICPWNPKQHPECDGTRT
                     EPADHLTKRMRNSNGMTSPEMSGTTFDEIYADDVLPKLVFSEVLRNLATAQRPVYIWS
                     AGYTYAFNALNREFLSAVANACDGNGKLNFECYPWTKPTEAEAVAHIEEMLVDSMIRA
                     NRLMPGYVNSSCYHLGAYSLPGRMSYDHYFYADYRWLVDRYWRTLATNPLFRGLAGIG
                     LYAYIYASEEDVRWLLATIRHYAIEGRTDSLADRYGFGYILPHLKNADFASGLDGWRV
                     SEGEPGCVIACQRKNFARGVLRYHDSKGESTCVLKRSAKEANTIAREIENLVPGRLYT
                     LRYAVHNTNGVFDVAVTLSGATDVTATTEVAANGQTSVVTKDLDVRTLVFRAEHEKAT
                     FSLTDWLDEKVPKRGIGEETLFGAIRVRPYYE"
BASE COUNT         1285 a         1531 c         1478 g         1159 t
ORIGIN      
        1 gcgtggcgcg gccgccgcag ccgatgacgc ccaacgtgac ggtggccgac ggtgcagtcg
       61 gatttgcacg cgcgacagcg gtgccggcga cggtggtgaa cgcagcggcg cgtaacaaaa
      121 atccgcgccg gttgattgaa gaacgcaaca tacagaatct ccctttgtga aaacagtata
      181 gaggagatgg gactcagggt ctaataaaac aacgatcgaa atgtgtatcc gtccgaatgg
      241 cgactaaaat cgacagccga gcccaagtcc gaaaatcgga tacgttgtct tggccattgg
      301 cacatctctt gcgttgatga gttcgcgcgc ctgcttgttg taaagcacat agtgccgata
      361 acgcccccag aggtaacagc tctctgtaaa ccgccattcc gtcaacaccc ccattaccgc
      421 aaacataaac gaaccgccaa gaaaatgatg atccgggccc ttgccgtagt tgcttctaaa
      481 cctgttgacg tcaccccatg tcgtttcggc atacggcgtg accgtgaatg agtccgccgg
      541 cttgaaaggt ttccgcaagc cgaaacacat acgcccattg gtggtcggtg tgacgcatcc
      601 aaggccgatc caatacggtg taacccatgg attgtgccaa tactgacggg cataccaggc
      661 aatgggcgtg cgcggcttct cttcatagcc cccataccaa tcccatagaa taccgccgtc
      721 tgtagacacc gtcaacgtct ctgcaagttt gacgtcatac ccatacagca gtgtcccctc
      781 ataaatatag aaagcgcggc gatgtttatg atccgtttga tcgttcagcg cactgcatat
      841 ccaagcactg ctggagaggc gaccgtaatt ccgcgactgc accgtaagag acgcgtattg
      901 ttccgccacg ggacgtgtat aggtgagcga cccgccggaa gagagatagg cggattcaaa
      961 atacgccctt acattgacgt gaacgtcaaa ctcttccgca tgaacattcg acgtaaacgt
     1021 gacgagaacc gctccgtaaa cagcgacgcc tagccaacga cggcccatat tgatcttctg
     1081 tgtcgcacag gaaatcactc ccgaaagtcc gcttaaaaaa cgttggcccc ggagccgtcc
     1141 gcctctaacc tacaggcgca tgaaaaacca agacgcttaa ccgcgcgact tcggaaccaa
     1201 tcgggacacc atacaccacc ctgcggcaga atgcaaacgg cagttgaaca ggcagaattc
     1261 gagagcaaaa tgtgccccgt cgaagtacac cgcctgcatc gagagtcccg ccgtgtcgtt
     1321 gttgaccgtg atcggcgtat cgcccgggtt tcggatgatc acgacattgc cgctcatcgg
     1381 cttcgcgtta ccctgccaat tcgccggatt ggaccggttg ccattgccag acgtccacac
     1441 gcgcgtttcg gcgaacagcg aacacgccaa aagcgccgcg ctgtcacatg ccactgtgcg
     1501 ctacaacgac gcactcgtgt tgcgctcgta tcgatgacgc acgtgctcat ctataatccg
     1561 taatgtcttt gaacaggcct tcgctatact cgtaaaaccg gatttggatt cgagggggat
     1621 tcttcttcgg atcagccctc cagccatgca tgtttccccc catggcattc accgtaaaga
     1681 acaccggaat ttcacgcctg gaaacgcttt tcggcacaag accaaaccct tcactcagac
     1741 acaacccgga gtgtttgcgg agggttccga tcatctttaa atcgaatgtc acacgtgacg
     1801 atcctccata atgaccgagg atgatttcct tcccatccgt acccattgtc tgaacgccat
     1861 atacgactga ataccccaat tgcagcgtct tttcctcctt tggattcaga ttcaagtcaa
     1921 atgtgcgaat gcagcaattg ctgtgcgcga ctttaccgaa tcgatctggg cccgcataca
     1981 atgtgtcacc tacgaccaac gcaccgtcga gggagtcgtc gaccgcacgt tcgtataacg
     2041 gatccaaatc ttcgttccag acacgtatga ggcctttgct gccatatttt ttgcgaatct
     2101 cttttccccg gagtgcaaag gctccgaata tctttccgtc cgcataagcg atgtccccaa
     2161 gatgcgccgg gacggtgatg tgctttttgt gaaccccgtt ccagtcaaag ctgtcaatgc
     2221 ccaattgatg gctcaggtaa aaccccttct cggagcagca agcaccttgg atgtgccccg
     2281 actcgatcag gcagtccaga tcatggggat gcgtctcgat cacgggtaac ttctctccgg
     2341 cccggacagt cagtccaagc aaaaagagga tgagacaacc ctgtaacacg gttctgctgt
     2401 tcatgaatac cccctacccg cttccagata tcatgtaaaa cggatgtgcc ataatctttc
     2461 agccctattt aaaaataatc acgctcatta ccattcagac aagacactgc catccatagg
     2521 gtaaatcgga aacacatccg tttacataat ccgcaaccat tatgcgggga gagggcaaaa
     2581 agagtcaacc cctgaagtcc gcgaaaacgt ttaggattgg gttggggctg aaatggactt
     2641 tttctggttt ctggaatgtg ttacagtttt ttcaatttgg gtaaaccagt tgtgtgaaag
     2701 aaaggaatga acgttccaat gaagaattct tgtttctcat tcgcgtgttg ctgcgttacc
     2761 tgtcgagggg tgcgggcggc ggtcatcgta tggatgtcgc ttgtggcgac cgtgacagtc
     2821 caagcgtccg ccttcgaaaa catcctgcat aaccccgaac tgcgccgcga taaaagcgga
     2881 tatgcctttg actggaattt cacctacgaa ggcttggagg attcgctgcg gttcggagat
     2941 gaagacggaa tcggtacagc cacgctgcta cccagcaagt tgagcacgtg cggggtgcgt
     3001 caggtcgaca tacgtctcaa atgcggcggg aaatacaaaa tcggcgctct ggtacgcacg
     3061 aagaactgca cgtgcacgca gggcgggatc atcatctata atcgggcatg ggtgaagtcg
     3121 gaaggcgtca gacccttccc gcgagataca gacggaaaat gggtacggct cgagaaagag
     3181 atcacggccc ccgagtcccg cggcggaatg tacgtgttca cggtttacgc ttcaaatctg
     3241 accgaaggag aaatctcttt ccgtgaaccc tatctcatcg cgcttgatgc cgagtccgac
     3301 gcggaagtgg tgcgcccccc taagctttcc gaactcaggg cgatcacgcc gattacaccg
     3361 cttctcggga agttgccgga aggcgacgcg accgtcacgt tctccttcct cgacgaaatc
     3421 gaaaaggatg tctgcaaggt cgcgcttaaa acggaaggcg acggcgattt ctgcgaaatc
     3481 ggccgtttcc cgatcatcgg cgggctcatc aaggcaaagc tccccgacct caaacccggt
     3541 cgcggctcca tccgggtcgc ggtttgcgac aagtcgggcg tggaggtggc cgcgaacgaa
     3601 tatccggttc gcgtgttggc acgggcggaa actccgcctg aggggcgcag acttaacgag
     3661 cttgtaaccc gcatcctgac tgcggagtcg aaagacgggg aattccgttt tcaggctccg
     3721 cgcgacggat ggatctggat ctcgctcgag cgcggcaacg agggaacgac agtgacgctt
     3781 gatgacggga cagagccggt cattatccac cgcgaaaacg aacgctacga aaccatgcgt
     3841 cacgtgaaga gcggcgaaca tgtcctcagc gtcaaaggtg cgacaggcgg aagcctcgtc
     3901 gtgaattcga ttccgatgat cgcgggattt accgtgccga tccgattctt caaggattat
     3961 tacgaggttt tcggcggcaa attctgccgc gagcggattt ttcccgtgat caacaccttc
     4021 agcataggct atccactgag ggtaatcggc gaggatgaac tcgccgacct caaatcgcgc
     4081 ggcaaggagc tgctcggaca gatctgcccc tggaacccga agcaacatcc cgaatgcgac
     4141 ggaacccgta ccgaacccgc agatcatctc acgaagcgga tgagaaacag caatgggatg
     4201 acatcgccgg aaatgagcgg cacgactttc gacgagatct acgcggacga cgtcctgccg
     4261 aaacttgtgt tttcggaagt gctcaggaat ctcgcgaccg cgcagcgtcc ggtctacatc
     4321 tggagcgctg gctataccta cgcctttaat gcgcttaacc gtgagttcct gtcagccgta
     4381 gccaatgcct gcgacggtaa cggcaagctc aatttcgagt gttatccctg gacgaaaccg
     4441 accgaagcgg aggctgtggc acatatcgag gaaatgctgg tcgattcgat gatccgcgcc
     4501 aaccggctga tgcccggata cgtgaattct tcctgttatc atctgggagc gtactctctc
     4561 cccggccgca tgagctacga tcattatttc tatgccgatt accgctggct cgtcgaccgc
     4621 tactggcgga cgctggcgac gaatccgctt ttccgcggtc tcgccggtat cgggctctac
     4681 gcttatattt acgcgtctga ggaggatgtc cgctggctgc tcgcgactat ccgccactac
     4741 gcgatcgagg ggcgcaccga ttcgctggct gatcgctatg gctttggcta tatccttccg
     4801 catctcaaga acgccgattt cgcgtccgga ctcgacggct ggcgcgtttc cgaaggtgaa
     4861 ccgggttgtg tgatcgcctg tcagcgcaag aactttgccc gtggcgtgct cagataccac
     4921 gattccaagg gggagtcgac gtgcgtgctg aaacgctcgg cgaaggaggc gaacacgatc
     4981 gcacgggaga tcgagaatct cgttcccggc aggctttata cactcaggta cgccgtccac
     5041 aacacgaacg gcgtgttcga tgtcgcggtg acactctcgg gagcaacgga tgtaactgcg
     5101 acgacagaag tggcggcgaa cggtcagacc tcagtcgtga cgaaggatct tgatgtgcgt
     5161 accctcgtct tccgtgccga gcatgagaag gcgacgttct cgcttaccga ctggctcgac
     5221 gagaaggtac cgaaacgcgg tatcggcgag gagacgctgt tcggcgccat ccgtgtcaga
     5281 ccgtattacg aatgaattca agccaaaagg agcagttgac cgggtagaat tctggcaaag
     5341 ctccgtaaac attgggtttt gtcgcggtcc ccgccgcacc gtattgagat gcgggcggag
     5401 cccgcaatct caaaggctgt gagactgtga ggctgttagg ctgtgaggtg gtg
//
LOCUS       DAOSRB010000182         5423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13753_length_5423_cov_7.793592, whole genome shotgun sequence.
ACCESSION   DAOSRB010000182 DAOSRB010000000
VERSION     DAOSRB010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13753_length_5423_cov_7.793592"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1764)
                     /locus_tag="PLZ74_09065"
     CDS             complement(298..1764)
                     /locus_tag="PLZ74_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98511.1"
                     /translation="MRILHGFFGTGLGLRHIIPMLAVLGGARAADYYVSPEGRDTDPG
                     TESSPWQTIAKASATMTAGDTVHIMKGVYRECVRPAHSGLPGQPITYTAYPGHSAVID
                     GIGVALAKRGGILGLVDIGNKKHIRISGLTVINTMEQNAAGIFAAGCEDLILENNRIA
                     NTGASGILTYRCSKVLIDGNRVQNVCVRGGQESISLMYTDTFDVRSNYVHSTPPSLTG
                     KGGWLYRKEGIDAKDGSSHGRIFGNHVHHTSLGIYVDARGTARDIEISGNIIHDIFSS
                     FEEPGGQGTGNGIVIASETEGTIENIRIFNNLIYNTHFGIRLAGYGDPGHPVMTNLTV
                     IHNTLYRTRRGIELSSRNATGIVIRNNLCSRSTDFDIGIFRNSWGDQPRMVLDDPTLC
                     VANNLLDVPAGAGRPWERDNLYGAANFVAPEAGDFRLRADSAARDRGTAAGMPSVDIT
                     GTPRPVGAAPDIGAYEFTGPGVKTESASPAIPAARPQP"
     gene            complement(1871..5251)
                     /locus_tag="PLZ74_09070"
     CDS             complement(1871..5251)
                     /locus_tag="PLZ74_09070"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98512.1"
                     /translation="MTFKHMLFLVPLASLAGTIQIPGSWSMPTEERDAVTLNALPPAV
                     PRKASGMRTTLAQASVRTAEDVSVAELKGFPGVRIAEGEIVLAYAPVTKTSAFAAMAK
                     SDATQKWYGSDYWIGSEGWARVGRDWMHPGHAVDVARTFIAPVNGRVSVSGTVRKLHL
                     DGDGVKAFVQHNNRTLWTAEVKGRDAVGQQHALTVEVKKGDTLRFLVNRLGSHACDTT
                     GWDPVVAYEGGGVHRASEGFSDKQEANGWRYEFSGGPAAQTSLPTIYGVGRDLKLYRN
                     AIDTVNALPFVIVSDGNKEDGFIGYVIARDSDAAWTLIRDPGGIKVAGAGAAVVVRYD
                     GTWIEGLTLLAALSGVETSPQRVRDGFGGAVLPKLGSVFRAAYAKAVAGVPHAPEPDL
                     FLLAVSEWVQDDQLFGCTDEQRLTACAKEQAERIEQITGKPDASAGTGWGGYLRARLL
                     KRAALLSDPRLAYGELLFCTRRNPAWNHEVAQYFGWRQRPGGSLFVLEKPGVSLMRRD
                     LINGRLPPGNLLEPRLSYDARSVLFSFVATDGALDPRTLPRNETDTNHHYYHLYEIGA
                     DGTGLRQITDDIYDDLMPEWLPDGGIAFVSTRRRTTSRCFWWSYSDRWHSYTMFRMAR
                     DGSDIRQLSWNDVNDWFPAVANNGHLLFARWDYIDRDAVTHQNLWSMRPDGTNPMAVW
                     GNEVPKPHCTFQAKAIPNSGKIVCVASAHHAITGGPIMLIDPAVHNNSEAAVTRLTPG
                     HYPEAERGSNNEWYNAPWPLGEDLFLVAYSRDPLMYEPARHNPDYALGLYVLDACGNR
                     ELLYRDGLIGATCPTPLRPRPVPPVLPSVLNPALAQREQGEVFISDVYEGIPKAKRGT
                     IKKIRVVQVFPKTTRDSMVPRIGVAGEENTRAVLGETPVEADGSARFLVPACKPILFQ
                     VVDADGCAYRVMRSSTSLMPGERVSCVGCHENKMAVSPAALKKVAAMMRPAAELTPTP
                     ESGRPYGFVEQVQPLFDAKCVSCHGDRNPKGGIALTRTLRGAYTASYDSLCYATDSEG
                     KRVRRLSKKSGRPLVTCYPMRNQIQVTPEGGEDSARGSGLFQMLKEGHGKVRLTPEEL
                     RRIAVWIDLNAVFYGVYEPEAQLAMQREGKPVPMPAIQ"
BASE COUNT          920 a         1697 c         1724 g         1082 t
ORIGIN      
        1 ttattgtgac cacgcagtgg ctcacaacga cgggggttca agggtttctg gtttctgggt
       61 ttcatggttt cggggtttca tctaaatcgg ggtcgggatc gctatcggga tcgggatcga
      121 ttattgtgac cacgcagtgg ctcacaacga cgggggttca agggtttctg gtttctgggt
      181 ttcatggttt catggtttca tctaaatcgg gatcgctatc gctatcggga tcgctatcgg
      241 gcggggcagt aagttcgggg caaccgctca tgcgcgcgcg aaaacctagg ggacggctca
      301 cggctgtggc cgcgccgcag ggatagcggg gctggccgac tccgtcttga ctccgggccc
      361 cgtgaactca taggcgccga tgtccggcgc ggcaccgact ggtcgcgggg tcccggtgat
      421 gtcaaccgac ggcatcccgg cggcggtgcc ccggtcgcgc gccgcggaat ccgcgcgcag
      481 gcggaaatcc ccggcttccg gggcgacaaa gttggccgcg ccgtagaggt tatcgcgttc
      541 ccatggccgt cccgcccctg ccgggacgtc gagcaggttg tttgcgacgc agagggtcgg
      601 gtcatccaga accatccgcg gctggtcccc ccacgagttc cggaagatgc cgatgtcaaa
      661 gtcggtgctg cggctgcaga ggttgttccg aatcacgatg cccgttgcgt tccgactcga
      721 taattcgatt ccccggcgtg tccggtacag cgtgttgtgg atcacagtga gattcgtcat
      781 gaccgggtgg cccgggtcgc cataccccgc cagacggatt ccgaaatgtg tattgtagat
      841 caggttgttg aaaatccgga tgttctcgat ggtgccctcc gtttcagagg cgatcacaat
      901 gccgttgccc gtgccctgtc cgccgggctc ttcgaacgag gagaaaatat cgtgaatgat
      961 gtttccggag atctcaatgt cgcgcgccgt tccgcgggcg tcgacataaa tgccgagact
     1021 ggtgtggtgc acatgattgc cgaatatccg tccgtgggaa gagccgtctt tcgcgtcgat
     1081 cccctccttc cggtaaagcc agccgccttt gccggtcaga ctgggcggcg tgctgtgcac
     1141 gtagttgctc cggacatcga acgtgtcggt atacatcaac gaaatgctct cctggccgcc
     1201 tcggacgcag acattctgaa cccggttgcc gtcgatgagg accttcgagc aacggtaggt
     1261 gaggatcccg gatgcgccgg tgttggcgat ccggttgttc tccaaaatga ggtcttcgca
     1321 cccggcggcg aagatgcctg cggcattctg ttccatcgtg ttgataacgg tcagtccgga
     1381 aatccggatg tgcttcttgt tgccgatgtc aaccagaccg aggatgccgc cgcgcttggc
     1441 cagcgcgacg ccgatcccgt cgatgaccgc cgaatgtccg ggatatgcgg tgtaggtgat
     1501 cggttggccc ggtaatccgg agtgcgctgg cctcacgcat tcccgataga cgcctttcat
     1561 gatgtgaacc gtgtctccgg cggtcattgt cgcggaagcc ttggcgatcg tctgccacgg
     1621 actcgactcg gtgcccggat ccgtatcccg gccctcggga ctgacgtagt aatccgctgc
     1681 gcgggccccg cccaaaaccg ccagcatggg gatgatgtgc cgaagcccca atcccgttcc
     1741 gaaaaacccg tgtagaatcc gcatgctttc ctcctttttt ccctgtcctt tccggtcccg
     1801 gtcgtgaccg gtgtcttaat cctgttcccc gcacacatcg gacgccacac gcttgggccc
     1861 cgccgcaggc tcactggatc gccggcatgg gcacgggctt gccctcgcgc tgcatcgcaa
     1921 gttgcgcctc gggctcgtac acgccgtaga agacggcgtt cagatcgatc cacaccgcga
     1981 tgcggcgcaa ctcctccgga gtcagcctga ccttgccgtg cccctccttg agcatctgga
     2041 agagtccgct gccacgcgca ctgtcctcgc cgccttcggg tgtgacctga atctgattac
     2101 gcataggata gcaggtcacc agcggacggc cactcttttt ggagaggcgg cgcacgcgct
     2161 tgccctccga atccgtggcg taacagagtg aatcatagga cgccgtgtat gcgccccgga
     2221 gcgtccgggt gagcgcgatg cctcctttgg gattccggtc gccgtggcag gagacgcatt
     2281 tggcgtcgaa gagcggttgc acctgctcga cgaagccgta cgggcgtccc gactccggcg
     2341 tgggtgtcag ctcggccgcg ggacgcatca tggccgccac ttttttcagt gccgcgggag
     2401 agacggccat tttattttcg tggcagccga cgcaggagac gcgctcgccg ggcatgagcg
     2461 aggtgctgga acgcatgacc cggtacgcgc aaccgtcggc atccacgact tgaaagagga
     2521 tcggtttgca ggcgggcaca aggaaacggg ccgagccgtc cgcctcaacc ggcgtttcgc
     2581 cgagtaccgc gcgcgtgttc tcctcgcccg ccacaccgat gcgcggcacc atcgagtccc
     2641 gtgtggtttt cgggaagacc tgcaccacgc ggatcttttt gatcgtgccg cgtttcgcct
     2701 tcggtatgcc ttcatagaca tccgagatga aaacctcacc ctgttcccgt tgcgcgagcg
     2761 cgggattcag gacagagggc aggaccggcg gcacggggcg cggccgaagc ggcgtcgggc
     2821 aggtcgcacc gatcaggccg tctcggtaaa gcagttcgcg attgccgcag gcgtcgagca
     2881 cgtagagccc gagggcgtaa tcaggattgt ggcgcgcggg ctcgtacatc agcgggtcgc
     2941 ggctgtaggc cacgagaaac agatcttcgc cgagaggcca gggcgcgtta taccactcgt
     3001 tgttggagcc gcgttccgct tcggggtagt ggcccggggt caggcgcgtc accgcggctt
     3061 cgctgttgtt gtgcacggcg gggtcgatca gcatgatcgg cccgccggtg atcgcgtggt
     3121 gcgcggaggc aacgcacacg atcttgccgc tgttggggat ggccttggcc tgaaaggtgc
     3181 agtgcggctt gggcacctcg ttgccccata cggccatggg gttggtgccg tcgggcctca
     3241 tggaccagag gttctggtgg gtgaccgcat cgcggtcgat gtaatcccag cgcgcgaaga
     3301 gcagatggcc gttgttggcg accgccggga accagtcgtt cacgtcgttc caggagagtt
     3361 gccggatgtc cgaaccgtcg cgcgccatgc ggaacatggt gtaggagtgc cagcggtcgc
     3421 tgtagctcca ccagaaacag cgtgaggtgg tgcggcggcg cgtggagaca aaggcgatgc
     3481 cgccgtccgg caaccactcg ggcatcaggt cgtcgtagat gtcatccgtg atctggcgca
     3541 ggcctgtgcc gtccgcacca atctcgtaaa ggtgatagta gtggtggttc gtgtcggtct
     3601 cgttgcgcgg caacgtacgc ggatcgagtg cgccatccgt cgccacaaaa gagaaaagga
     3661 cggagcgggc gtcgtaggac aggcgcggtt cgagaaggtt accgggaggg agccttccgt
     3721 tgatcagatc tcgccgcatc agggagacgc cgggtttctc cagcacaaac aggctgccgc
     3781 ccggccgctg ccgccaaccg aaatactgtg ccacctcgtg gttccaagcc ggattgcggc
     3841 gcgtgcagaa gagcaactcg ccgtacgcga gtctgggatc ggacaacagg gccgcgcgct
     3901 tcaggagccg cgcccgcagg tatcctcccc atcccgttcc tgccgacgcg tcgggtttcc
     3961 ctgtgatctg ttctatccgt tccgcctgtt cttttgcgca cgcggtgagc cgttgctcat
     4021 ccgtgcaacc gaagagttgg tcatcctgca cccactccga gaccgccagc aggaacaggt
     4081 ccggctccgg cgcatgcggc acgccggcca cggccttcgc ataggcggcg cggaacacgg
     4141 agccgagctt cggcaggacg gctcccccga aaccgtcgcg gactctttgg ggagacgttt
     4201 ctaccccgga caaggcagcc agcagcgtaa gcccctcgat ccatgtgccg tcgtaacgca
     4261 caaccaccgc cgcgcccgca cccgccacct ttatcccccc cggatcgcgg atcagggtcc
     4321 aggcggcatc cgaatcccgt gcgatcacat agccgataaa gccatcctcc ttgttgccgt
     4381 ccgagacgat cacaaaaggc agggcgttta ccgtgtcaat cgcgttgcgg tagagtttca
     4441 gatcccggcc caccccgtat atagtgggca gggaggtttg cgcggccggt cctcccgaaa
     4501 actcgtaacg ccagccgttc gcctcctgtt tgtccgaaaa gccttcggag gcccggtgta
     4561 cgcccccgcc ctcgtacgcg acgaccggat cccagcccgt agtgtcgcac gcatgggacc
     4621 ccagacgatt gacgaggaag cggagcgtgt cccctttttt gacttcgacc gtgagcgcgt
     4681 gttgctgccc gaccgcatcc cgccctttca cttccgctgt ccagagggtc ctgttgttgt
     4741 gttgcacaaa ggccttcacg ccgtcgccgt cgaggtgtag tttcctgacc gttccggaga
     4801 ccgaaacgcg cccgttgacg ggcgcgataa aggtgcgcgc cacatcaacg gcatggccgg
     4861 gatgcatcca gtcccttccc acgcgggccc acccttcgga accgatccag taatcagagc
     4921 cgtaccattt ttgcgtcgcg tctgatttgg ccatggccgc gaaagcgctg gttttcgtca
     4981 ccggggcgta ggccagcaca atttcgcctt ccgcgatccg cacgcccgga aaccctttca
     5041 gttccgccac ggacacgtcc tctgctgtcc gcacggaggc ctgcgcgagt gtggtccgca
     5101 tgccggacgc cttgcgcggc acggctggcg gcagcgcatt tagtgtgacg gcatcgcgct
     5161 cctccgtcgg catcgaccag gaacccggga tttgaatcgt gcccgccagc gaggcaagcg
     5221 gcacaagaaa cagcatgtgc ttgaaggtca tcgtttttct cctcgaattc gttttcttcc
     5281 tcactcgatt ccctgcgcgt tcgcgcggaa aggtcccggc cggatcacga tcggtgcggt
     5341 tttcaagcgc ggccggatgg ccagccagtc ggagccttgg aactccggat catcatcggc
     5401 gcggatctcc acgtccgtaa gat
//
LOCUS       DAOSRB010000183         5376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13911_length_5376_cov_4.775606, whole genome shotgun sequence.
ACCESSION   DAOSRB010000183 DAOSRB010000000
VERSION     DAOSRB010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13911_length_5376_cov_4.775606"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLZ74_09075"
     CDS             complement(<1..743)
                     /locus_tag="PLZ74_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOR98513.1"
                     /translation="MIRPAAHKAIVILDFDGTLYRGLCPALCRGIANADLLVALCLIN
                     LSRPRYFFRLLREATRLQKLQQRLARAYTEKRLSLSSADGRLIRFFTHRIMMCCTPTV
                     LDQAAALVSRLCYPSAWRALSAVRGDCDFAVVSKSFDFLLEKVRQRAATRGVHLQVVG
                     TRVRCGGAGWRIEVPMTRAEKALQVRELLMGRAYDRAVVIGDTEDDIGMRDAAVKVLG
                     AESVTFIALNVKDNEIKSAADVSCPSWRQV"
     gene            complement(740..1549)
                     /locus_tag="PLZ74_09080"
     CDS             complement(740..1549)
                     /locus_tag="PLZ74_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR98514.1"
                     /translation="MSDGRLPEKAELTVVHLTDLHLDTQGKVTHTPWTHRIAVGGYRL
                     HRPCTGRAFEYLEQAVSQIRDTVKPDVVVITGDVVNRGDDIEALRRGVSILSRLQCPV
                     VVVQGDHDIPRRKENGTVWNDLFDTTHGCTLVNNTRFFFLPFVKDETAFADTLQAMRT
                     VSRRDKIHFFCLHRMLWAPRLMDALARRYHGCSVLDPDREKIVEAMDTSGARWMVLCG
                     HSHTDYCRTRGNITQLCAPSLAEHPHAFRVIKIKDGQVFYKLVRLDKGKIK"
     gene            2277..4400
                     /locus_tag="PLZ74_09085"
     CDS             2277..4400
                     /locus_tag="PLZ74_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HOR98515.1"
                     /translation="MCKSFLKRLLPVVVCGLSACGHAAPTKTLVAWVYLDNTTQQGGS
                     ALTIQRGKQFDAIVFGEKAAGKWMAGSNYFRRTQDDQQSNAAETAAADTLIQMAIVYT
                     EQRITIWRNGEMYADYAAANIDLLSAPDNIAVFGLRHEGATTRQTLAGKIEDARIYDR
                     ALTQAEISALRPDQPSTIKPYAWWTFEPGQETDRAGRFAINVLSGGAKIEDGMLKLQS
                     GGKLVAHMPASSVPPEQLAMPENPPATWLTYHLAHPGPSGARPGDPNPAFYYRGRYHL
                     HYIYNHKDGFAYAHVSSTDMVHWKWHPTTITPKTMGHGMFSGTGFFTRNGTPAMIYHG
                     HGSGRNQLAFALDDNLEHWSQPQAIEPFTAAGEKPKMRHWDPDCWFMDNAFYALSGGE
                     NPKLMKSADLRNWLYLGDLFHESFPTDLGVAKGEDVSCANMFRLGNKWMLLCISHGLG
                     TRYYLGDFIDEKYLPSHHGMMNWAKWDCFAPESLLTPDGRRVMWAWCTPWTNTMQRVG
                     RSKNFDALLNQAVFQQGIQSLPRELSLPEDGVLRIKPLRELEALRYDRQEQRKITVPG
                     EAQQLLSGISGDTLELEVVFSARQAQKFGINLLCDAKGDNGFKISCDAVSGNLNVGYV
                     TPSFQVKASEDLTLRLFIDKSMIEVFVNERHAVVAWHEYPPDNRHISLFAKGGNLLVK
                     KMTAWKLKSIYAGNCVFDPAASKKQ"
     gene            4614..5087
                     /locus_tag="PLZ74_09090"
     CDS             4614..5087
                     /locus_tag="PLZ74_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOR98516.1"
                     /translation="MENTIEARVVEICRERKQTLVTAESCTGGLVACRITSVPGASDI
                     YLGSVVSYANAVKCGMLGVTPEILARDGAVSAVCAEAMACGVRERLGADLAVAVTGVA
                     GPDGGTLQKPVGLVFLAVATTAGVRSEACRFGGNRAEVRMRAADRALTLVLESLG"
BASE COUNT         1161 a         1509 c         1587 g         1119 t
ORIGIN      
        1 acctgtctcc aagaaggaca ggagacatcg gcagcgctct ttatctcatt atccttcacg
       61 ttcaaagcga taaacgtaac gctctcggca ccgagaacct ttacggcggc atctcgcatg
      121 ccgatgtcat cttctgtgtc accgatcacc actgcccggt catatgctct gcccatcaac
      181 aattcgcgca cctgcaaagc cttctccgcg cgagtcatgg gcacctcaat gcgccagcca
      241 gcaccgccgc atcgcacacg cgtgccgaca acttgcagat gcacaccacg tgtcgccgct
      301 cgctgccgga ctttctccag aagaaaatcg aaggatttac tgaccaccgc aaaatcgcaa
      361 tcgccacgaa cagcactcaa cgcacgccaa gccgacgggt aacacaaccg agataccagc
      421 gcggcagcct gatccagtac ggtaggcgta caacacatca ttatccggtg tgtgaaaaag
      481 cgtatcagtc gtccatctgc actggaaagg ctgagtcgct tttccgtgta ggcacgggct
      541 agccgctgtt gtaacttctg gagccgcgtg gcttcacgca agagccgaaa gaagtagcgg
      601 gggcgcgaca gattgatcag gcagagcgca accagcaggt cggcattcgc gatgccgcga
      661 cacagcgcgg gacacaaccc gcggtagagg gtgccgtcaa aatccagaat gacgatggct
      721 ttatgcgctg cgggtcttat cacttgatct ttcccttatc cagtcgcaca agcttgtaaa
      781 agacctgtcc gtctttgatc tttataacgc ggaaggcatg cggatgttca gccaagctgg
      841 gcgcgcacag ttgcgtgatg tttccccgcg tccggcagta atctgtgtgg gagtgcccgc
      901 agagcaccat ccatcgcgca ccggaggtgt ccatcgcttc gacgattttt tcccggtccg
      961 gatcaagaac ggagcagccg tgataccgtc tggccaaagc atccatcaga cgcggtgccc
     1021 acagcattct gtgcagacag aaaaaatgaa ttttgtcccg acgggaaacg gtacgcatgg
     1081 cttgcagagt atcggcgaag gcggtttcgt ctttcacgaa cggcagaaag aaaaagcgcg
     1141 tgttgttgac aagtgtgcaa ccgtgcgtcg tatcgaacaa gtcgttccag accgtgccat
     1201 tctccttccg tctcggaatg tcatggtcgc cttgaacgac gacaaccgga cattgaagac
     1261 ggctcaaaat ggaaacgccg cgtcttaacg cctcgatgtc gtctccgcgg ttcacgacat
     1321 cgccggtgat cacgacaaca tccggtttga ccgtgtcccg aatttgtgag accgcctgct
     1381 ccagatactc aaaggctctt ccggtacagg gccgatgcag ccggtaaccg cccacagcaa
     1441 tcctgtgtgt ccacggtgta tgcgtgactt tgccctgtgt gtccagatgc aggtcagtca
     1501 gatgaacgac cgtcaactcg gccttttcag gcagacgtcc gtcactcatg cttgagtgcc
     1561 caaaacacac tccaagaaag aggatgacag ctccccagaa aagccactgc tttttgttgt
     1621 ttcttcgtgt tgacattttc ggggtatagt gcttcacaat gcgggaacat acgcttcgtc
     1681 gcttacgctt agcccacatg ctcattccag ccgcctcgtc gctgttactt taccggaaca
     1741 gccgtctaaa tgcaatcgac caaagtggtc gtcgaacgtc ctcgcgggag gctagtcgaa
     1801 ttcccttcgc atttccgcgt acgtgaacgt gtgcgacggc atccgaggat atgcgtttgc
     1861 ttgcccgtga ggacggttcg ccgaaccgtc cgcggacgcc ccggggaggt gtccctgccg
     1921 gagcccggca cggttggttt cggcggcccg gctccgcgcc ggtggcgccg tagctgtgga
     1981 cgtcccgccg tactggcagg aaccgtccgc agttcgcggg gacgcccgtg gggataataa
     2041 atgtccacgt tatcggtgga aagggcgagt tccggaacct gagttttcaa cgcgcagtga
     2101 cttggggaga tgtccgccgg acttgcggag acgcggcatg gagagtatag taccgatgcg
     2161 atgcagggag tggaaaacac gatcgaggct ttgaatggca aagcattttt cgcgctatct
     2221 ccatccagca cattcacaca cttaggatgc gggctctgcc cgcgttagga gtcgccatgt
     2281 gcaaatcttt tttaaaacgc ttgctgccgg ttgttgtttg tggtctgtcc gcatgcgggc
     2341 atgccgctcc aaccaagact ctggtggcct gggtctacct tgacaatacc actcagcaag
     2401 gcggcagcgc gttgacgatc cagcgtggca aacagtttga tgccatcgtg tttggcgaga
     2461 aggcggcagg caaatggatg gcgggcagca actatttccg gcgtacgcag gacgaccagc
     2521 aaagcaatgc cgcggaaacg gccgcagccg acaccttgat ccaaatggcc atcgtctata
     2581 ccgagcagcg cataaccatc tggcgcaatg gcgagatgta cgcggattac gcggcggcca
     2641 acatcgatct gctcagcgcc cccgacaata tcgcggtttt cggattgcgg cacgaaggcg
     2701 ccaccactag gcaaacgcta gccggcaaaa tcgaagacgc acgtatctat gaccgcgccc
     2761 tgacacaggc tgaaatcagc gcattgcgcc ccgaccagcc ctcgacgatc aaaccctatg
     2821 cctggtggac ctttgagccg gggcaggaaa ccgaccgggc gggacgcttc gcaataaacg
     2881 ttttgtccgg cggcgcaaaa atcgaggacg gcatgctgaa actgcaatcc ggcggcaaac
     2941 tggtggctca tatgccagcc agctccgttc ctcccgaaca actcgccatg ccggaaaacc
     3001 ctcccgccac ttggctgact taccatctgg cccacccggg acccagcggt gcgcgtccgg
     3061 gcgatcccaa tccggctttc tattacagag gcagatatca tctgcactac atctacaacc
     3121 ataaagacgg cttcgcctac gcgcatgttt ccagtacgga tatggtgcac tggaaatggc
     3181 atccaaccac aatcacccca aaaacgatgg ggcacggcat gttcagcggc accggtttct
     3241 tcacccgaaa tggaacaccg gccatgatct accacggtca tggctccggc cgcaaccagc
     3301 ttgcgtttgc actggacgac aacttggagc actggtccca gccccaggcg atcgagccgt
     3361 ttaccgctgc cggtgaaaag ccgaagatgc ggcattggga tccggactgc tggttcatgg
     3421 acaatgcctt ttatgcattg agcggcggcg agaacccgaa actgatgaaa tctgccgacc
     3481 tccggaactg gttgtacctt ggcgatctct tccatgaatc gttccccacc gatctgggtg
     3541 tcgccaaagg cgaggatgtc tcatgcgcca acatgttccg gttaggcaac aagtggatgt
     3601 tgctctgcat cagccacggt ctgggtaccc gctactatct gggcgacttc atcgacgaga
     3661 agtacctgcc cagccatcac ggaatgatga actgggccaa atgggactgc ttcgctccgg
     3721 agtcgctctt gaccccagac gggcgtcgtg tcatgtgggc ctggtgcaca ccctggacga
     3781 atactatgca gcgtgtcggg cgctccaaaa atttcgatgc gctgctgaat caagccgtct
     3841 ttcagcaggg catccagtca ttgccgcgcg aactgagcct cccggaagac ggtgttctgc
     3901 gcattaaacc attgcgcgaa ctggaggcgt tgcggtatga ccggcaagag cagagaaaga
     3961 tcaccgtgcc cggagaggcg caacagttgc tgtcggggat cagcggagac acgttggaac
     4021 tggaggtggt tttttccgcg cggcaagcac agaagttcgg catcaacctt ttgtgcgacg
     4081 ccaagggtga taatggattt aagatttctt gtgacgctgt cagcggcaat ctgaacgttg
     4141 gttatgttac cccgtctttt caggtcaaag cctccgagga cctgaccctg cggctcttca
     4201 tcgacaaaag catgatcgag gtttttgtca acgaacgcca cgccgtcgtg gcatggcatg
     4261 agtatccgcc cgacaaccgg catatcagcc tgtttgccaa aggcggcaac ctgcttgtta
     4321 aaaaaatgac cgcctggaaa ctcaagtcga tttatgcggg taattgcgtg tttgacccgg
     4381 ccgcctcaaa aaaacagtga ttgaaatcac gtagcgatat ggattccggt gtcagttggt
     4441 ttgggctttg ggatcgaagt cgacacgctt gacttgacac tgcgcggtta ccgcgtgcga
     4501 ccgatctgca cgtcacgtcc gggcgcaacg gtcaacggcc agtgactcgg ggggtgtcca
     4561 ccggacttgc ggaggcgcgg tacggggagt atagtacaga tgtgatgcaa ggaatggaaa
     4621 acacgatcga ggcgagggtg gtggagattt gccgggagcg gaaacaaacg ctggttacgg
     4681 cggagtcctg tacgggcggt ctggtggcct gtcggatcac gtccgtaccg ggggcttcgg
     4741 atatttatct cggtagcgtt gtgagttacg ccaatgcggt caaatgcggc atgttgggcg
     4801 tcacgccgga gattctggcg cgggacggtg cggtcagcgc ggtctgtgcg gaggccatgg
     4861 cctgcggggt gcgcgaacgg ctgggtgccg atctggcagt cgcggtcacg ggagttgcgg
     4921 ggccggacgg cggcacgctt caaaagccgg tcgggctggt gtttttagcg gtggcaacca
     4981 ctgcgggcgt gcgctccgag gcctgtcggt ttggcggtaa ccgcgcggag gtgaggatgc
     5041 gggcagcgga tcgggccctg actctggttc tggagagttt gggttgaacg gatgggattg
     5101 cgctgttggt cgtcccggaa aggggggggc tcctgttttt catcgacttg ggcaataaaa
     5161 tatatcaaaa tacattattc acacgggatt ctcttttttc cccgaagcgg aatgcggtta
     5221 tgaagtgttt tgaatttgtt gaaggcgggg ttacggcggc gcgcgggttt cgcgcgtgcg
     5281 ggatcgaggc gggcatcaag gacgccgggc gtaaggactt ggcgatgctg gtggccgacg
     5341 cgccagcggc ggtggcggcc gtatacacga ccaata
//
LOCUS       DAOSRB010000184         5365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13948_length_5365_cov_7.681921, whole genome shotgun sequence.
ACCESSION   DAOSRB010000184 DAOSRB010000000
VERSION     DAOSRB010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13948_length_5365_cov_7.681921"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..3816
                     /locus_tag="PLZ74_09095"
     CDS             415..3816
                     /locus_tag="PLZ74_09095"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOR98517.1"
                     /translation="MKNSVFLWVAVTAMVIGYALAVRGAAVSVKIAEAAVVWNPETGF
                     ARYGKGRCQMALAGFTNMIDKVTGRVPAVYVEGQEPADLVSVIYFGNTKAARAAGCDA
                     QELRRGDWRIKSVPGKVFIFANSGMGLAYAAADFAERHCGYYYLTPEGDDPYDFNPAL
                     TIPVGDITVKPAIYNAKVYTAVGGTWNRWAINRRLSSTDEMEGQHRDSYEVRDFTGRT
                     RTCHTSFCYLPPEKHFKDHPEYYSLIKGKRQYTPVGQLCMSNEEVKRLCLEQLLTFIA
                     EDRRKYPDNYPLVYDFTQQDNMSFMCECPECKKIIDKYKRTSGDKNRTDNYWGGDAGL
                     QLEFINWIADRVAEKYPDVLIRTFAYVSTETPPKPGTIKPSPNVCIWWCDLYGESEHL
                     RPITAKPFNYKRGEQIREWLQLAKSVQVWDYMLYGGSHSGDFPEFAPDAIKADAKFFA
                     ENGLDDLFMESERSGIMPQPFYGLNYYLMSKLYTDPDEDVDALIKTYCRVYGKGAAKM
                     YEALQFLRRIEIENPAQTPGEWHQRVLPWRTRANGEAFRALVKSAYDQEGPGAARARM
                     ARVLSSVSRDLVRTLGQIPSAAAELKTAKKDFADYSFEYAKTASMTDQERKKMLADIQ
                     TEVELFDLKFKNIPKEVEGVPEDEIKCADYRRFSGSPNGKRMDDPDSETGKAFVWKYG
                     KPDAEHMGFPVSCGVYDGARKKNCAFKITAEMAGTDEQYHWIKLGKGWVGPQGMFWIP
                     HDWHMSFRFADWHILCDGLPSDPNWYDVYLSAKFTGPAYVPGSTRPNGIWIDRLLLRR
                     TQPPKENIVSPAKPGRADWMRGKYGLMVHWLFPSNRDIDRWTDAFNVDAFLKDFDKTG
                     AEWLIFTAGQCRGAYCSPSETFTKFCGPGHVPKRDLLAEIACGVKARGKRFIVYAACD
                     FVKDGCDDHSVQKGLCWDNSTEDRTEFERRWPLVLKEWAIRLEKNCDGWWLDGLGVQY
                     PNGVNWKLWCEACRAGNPAAAIAFNSGANNFSAYGPCDYTAGEASALKFALPDLDTAP
                     PAAAVRHHLFPIDGYWGGFWTWPTASWAGVQHLMPTRPELFDKAAMDARLEKRDFPDP
                     IYDVATLGAYLRKARETGSAATINIGISPEGRLNPKSIELIRAAR"
     gene            complement(3906..>5365)
                     /locus_tag="PLZ74_09100"
     CDS             complement(3906..>5365)
                     /locus_tag="PLZ74_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98518.1"
                     /translation="HQSGEPECFRSFNAGAVPMSDGNRRDFLKGTAWMLSAAAAGCVN
                     NRPFGEYPSVANFTVPPMTKVRIGFVGVGERGTWAVKRISNIPGTETAALCDLRPQQI
                     PPLIEYLNKKKSPGTLHTYAGKDDSWKGLCDDAAVDIVYLCTPADLHARMAVYAMNAG
                     KHVMVEVPAAKTLDDCWAIVEASERNRVHCMMLENCVYGENELLAWNLCHQGLLGTLT
                     HAEGGYIHLLTWRHLENHFRNRHHQKPGTVVHTYGNSYPTHALGPICTYMDMNRGDRM
                     EKVVSMSSLSAGHREFARHFYKPDDWQNNIEWRTGDMNTSIIRTALGRTIMMQIDMST
                     PRPYSRINLIQGTKGCFYDYPLRITYSDEPGGQKGHEWFDKKKLENVRKLHMHPLYKD
                     AGEIAKKIGGHGGMDFMMDLRWVFCLQNGLPLDMDVYDLASWSSIVPCSADSDRRGGE
                     VVELPDFTRGAWKTAKPISIGSIDITKMGLENAHR"
BASE COUNT         1226 a         1566 c         1453 g         1120 t
ORIGIN      
        1 cgatcccgat ttggatgaaa ccatgaaaca catgaaactt catgaaaatc agggactccc
       61 cttggcgttc cccaaaaacc cgagcgatgc cccaaaaatc tgcgtaatct gtgtaaatct
      121 gtggataaaa acccgagcag tgccatcccg tgagttcgtg gttaaataat cgattccgat
      181 agcgatagcg acaccgatcc cgatttggat gaaaccatga aaccttgaaa ccttgaaacc
      241 atgaaaccag aaaccctgaa accatgaaac catgaaaccc tgaaaccaaa gaaagttgaa
      301 ggttaaactg ctctttttag gattgactct tttcaccccc ctccgcatag taagcggcga
      361 tcatggcaac cggtgtgttt ccggtctacc ctgacatcca ttacgaagtt ttttatgaaa
      421 aattcagttt ttctctgggt cgccgttacg gcgatggtca tcggatacgc tctcgccgtg
      481 cgcggggccg cagtgtcagt caagatcgcg gaagcggccg tcgtctggaa tcccgaaacc
      541 gggttcgcgc gctacgggaa aggacgctgc cagatggcgc tcgccggttt caccaacatg
      601 atcgacaagg tgacaggcag ggtccccgca gtctacgtcg aggggcagga accggctgac
      661 ctcgtgtcgg tgatctattt cggcaacacg aaagcggcgc gggcagccgg atgcgacgcc
      721 caagagcttc gccgcggcga ttggcggatc aaatccgttc ccggcaaggt cttcatcttc
      781 gccaattcgg gaatggggct cgcgtatgcc gcggccgatt tcgccgagcg ccattgcggc
      841 tactactacc tgacgccgga aggggacgat ccgtacgatt tcaatccggc gcttacgatt
      901 ccggtcgggg acatcacggt caagcccgcg atctacaacg ccaaagtcta taccgcagtc
      961 ggcggaacat ggaaccggtg ggcgataaac cggcggctgt cctccacgga cgagatggag
     1021 gggcagcacc gcgattccta tgaggtcaga gacttcacgg gacggacacg tacctgccat
     1081 acgtcgttct gttacctgcc gccggagaaa cacttcaaag accacccgga atactactcg
     1141 ttgatcaaag ggaagcgcca gtacacgccg gtcggacagc tctgcatgtc caacgaggag
     1201 gtgaagcggc tctgcctcga acaactcctc acgttcattg ccgaggaccg ccgaaaatat
     1261 ccggacaact atccgcttgt ctacgatttc acccagcagg acaacatgag tttcatgtgc
     1321 gagtgcccgg agtgcaagaa gatcatcgac aaatacaagc gcacgtccgg cgacaagaac
     1381 cgcaccgaca attactgggg cggtgacgcg ggattgcagc ttgagttcat caactggatc
     1441 gcggacaggg ttgcggagaa gtatccggat gtcctcatcc gcacctttgc ctacgtctcg
     1501 accgagacgc caccgaaacc cggcacgatc aagccgtcgc cgaacgtctg catctggtgg
     1561 tgcgacctct acggcgaatc cgaacacctg cggccgatta ccgccaagcc gttcaactat
     1621 aaacgcggcg agcagatccg cgagtggctc cagctcgcga agtctgtgca ggtttgggac
     1681 tacatgctct acggcgggtc gcattcgggc gattttccgg aattcgcgcc ggacgcgatc
     1741 aaggcagacg cgaagttctt cgcggaaaac ggtttggacg acctgttcat ggagtccgag
     1801 cgcagcggca tcatgccgca gccgttctac gggcttaact actacctgat gtcaaagctc
     1861 tacacggatc cggatgaaga cgtggacgcg ctcatcaaaa cctactgccg cgtttacggc
     1921 aagggcgccg cgaagatgta tgaggcgctg cagttcctcc gccggattga gattgagaac
     1981 cctgcccaaa cgcccggcga atggcatcag cgtgtcctcc cgtggcggac ccgtgcgaac
     2041 ggggaggcgt tccgcgcgct cgtgaagtcc gcttacgatc aggaaggccc gggtgccgcc
     2101 cgcgcccgta tggcacgcgt cctttcctcg gtgtcgcgcg atcttgtccg taccttaggc
     2161 cagatcccct cggccgcggc ggagttgaaa acggcgaaga aggatttcgc cgattattca
     2221 tttgaatacg cgaaaactgc gtccatgacg gatcaggaac gcaagaagat gctcgccgat
     2281 atccagaccg aggtggaact ctttgatctc aagttcaaga acattccgaa ggaagttgaa
     2341 ggggtgccgg aggatgagat caagtgcgcc gattaccgtc gtttcagcgg ttccccgaac
     2401 ggcaagagga tggatgatcc tgattccgag accggcaagg cgttcgtctg gaaatacggc
     2461 aagcctgatg ccgaacatat gggattcccg gtatcgtgcg gagtttatga cggcgcgcgc
     2521 aagaagaact gcgccttcaa gatcacagcc gaaatggccg gtacggatga gcagtaccac
     2581 tggatcaagc tcggcaaggg ctgggtgggc ccgcagggta tgttctggat accccacgac
     2641 tggcacatga gtttccgttt tgccgactgg cacatcctgt gtgacggtct tccgtcagac
     2701 cccaactggt acgacgtcta cctttccgcc aaattcaccg gccccgccta tgttcccggc
     2761 tcaacgcgtc cgaacggcat ctggatcgac cgtctcctcc tccgccgcac gcagccaccg
     2821 aaggaaaaca ttgtgtctcc agcgaaaccg ggacgggcgg actggatgcg cggcaaatac
     2881 ggcttgatgg tccactggct ctttccgtcg aaccgtgaca ttgaccgctg gacggacgcg
     2941 tttaacgtcg atgcgtttct gaaggacttt gacaagaccg gtgccgaatg gctcatcttt
     3001 accgctggcc agtgccgcgg cgcctattgc tcgccgagtg aaacattcac gaaattctgc
     3061 ggacccggtc atgtgcctaa gcgtgacctg ctcgccgaaa tcgcctgcgg cgtgaaggcg
     3121 cgcggcaaac ggttcatcgt ctacgccgcc tgtgattttg tgaaggacgg atgtgatgac
     3181 cattctgtgc agaaaggact ctgctgggac aactccactg aagaccgcac cgaatttgaa
     3241 cggcgctggc ccctggtgct gaaagaatgg gcgatccggc tcgaaaaaaa ctgtgacggc
     3301 tggtggctcg acggtctcgg ggtacaatat ccgaacggcg tcaactggaa gctgtggtgc
     3361 gaggcctgcc gcgccggcaa ccctgcggcg gcgatcgcgt tcaactccgg cgcgaacaat
     3421 ttttccgcct acggtccgtg cgactatacg gccggcgaag cgagcgcgtt gaagttcgcg
     3481 cttccggatc tcgatacggc tccccccgcc gctgcggtcc gtcaccacct tttcccgatc
     3541 gacgggtact ggggagggtt ctggacctgg cccacggcct cgtgggccgg cgtccagcac
     3601 ctgatgccga cacgtcccga gctttttgac aaggcggcga tggacgcgcg gcttgagaaa
     3661 cgcgatttcc cggatccgat ctacgacgtg gcaacgctcg gtgcttacct ccgcaaggca
     3721 agggagaccg ggtcggccgc caccattaac atcggcatct ctccggaagg tcggctcaat
     3781 ccgaaatcaa tcgaactcat ccgcgccgcg cggtaaagaa agacggcatc tgggagcatt
     3841 gataagtgcg atgagcaacg attgtttgtt ttcttgcagg gtgctttcaa agatcgcaag
     3901 gtcgtttatc tgtgcgcatt ctccagcccc atttttgtaa tatcaatcga gccaatgctg
     3961 atcggtttcg ccgttttcca tgccccgcgg gtaaagtcag gcaattcaac aacttccccg
     4021 ccacgccgat cactatcagc ggaacacggc acaatggaac tccaactggc gagatcgtaa
     4081 acgtccatgt ccagcgggag tccattctgt aagcaaaaca cccaacgaag atccatcatg
     4141 aaatccatgc cgccatgtcc gccgattttc tttgctatct cacccgcgtc cttatagagg
     4201 ggatgcatat gcagttttcg tacattctca agtttctttt tgtcgaacca ctcatgcccc
     4261 ttctgtccac ccggctcgtc actgtaagta atgcggagag gatagtcata aaagcacccc
     4321 ttggtgccct gtatcaaatt gatgcggctg taaggacggg gtgttgacat gtcgatctgc
     4381 atcataatcg tacgccccaa agctgtccga atgatcgacg tattcatgtc ccccgtccgc
     4441 cactcgatat tgttctgcca atcatcgggc ttataaaaat gccgcgcaaa ctcacgatga
     4501 ccggcagaca gcgacgacat cgaaacgacc ttctccatcc ggtcgccacg attcatgtcc
     4561 atataggtgc aaatcggtcc gagagcatgt gtcgggtatg agtttccata tgtatgaacg
     4621 accgtgcccg gtttttgatg atgccgattc cggaaatgat tctccaagtg acgccacgtt
     4681 aaaaggtgaa tatacccgcc ctcggcatgt gttagcgttc ccaagagacc ttgatggcac
     4741 aggttccatg ccagcagctc attctcacca tatacgcagt tctccaacat catgcaatgc
     4801 acgcgattgc gctcgcttgc ctcgacaatc gcccagcagt catcaagggt cttggcagca
     4861 ggaacctcga ccatgacatg cttgcctgcg ttcatcgcat acactgccat gcgtgcatga
     4921 agatccgctg gcgtgcaaag atacacgatg tcgacagcag catcgtcaca cagtcccttc
     4981 caactgtcat ccttcccggc gtaagtgtgc aacgttccgg ggcttttctt tttattcaaa
     5041 tattctatca gtggcggaat ctgctgggga cgcaaatcgc aaagagcagc cgtctcagta
     5101 ccggggatgt tgctgatgcg tttaacagcc cacgtgccgc gctcaccaac acctacaaaa
     5161 ccgatccgaa ccttagtcat cggaggaacg gtaaaatttg caacggatgg atattcgcca
     5221 aaaggccgat tgtttacaca cccagccgcc gccgcgctca acatccacgc cgtgcccttt
     5281 aagaaatcac gtcgattccc atcgctcatt ggaactgctc ctgcattaaa agaacggaaa
     5341 cattccggct cccctgattg atgcc
//
LOCUS       DAOSRB010000185         5349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_13999_length_5349_cov_7.110880, whole genome shotgun sequence.
ACCESSION   DAOSRB010000185 DAOSRB010000000
VERSION     DAOSRB010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13999_length_5349_cov_7.110880"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLZ74_09105"
     CDS             complement(<1..199)
                     /locus_tag="PLZ74_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOR98519.1"
                     /translation="MEMSKNSMPLLGDKFPELSVKTTQGPMNIPGDLKGSWFVLFSHP
                     ADFTPVCTTEFVAFQKRYDQFK"
     gene            515..1792
                     /gene="hemL"
                     /locus_tag="PLZ74_09110"
     CDS             515..1792
                     /gene="hemL"
                     /locus_tag="PLZ74_09110"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOR98520.1"
                     /translation="MRRYKVDHEELYTRACRVIPGGVNSPVRAFNAVGGVPLYVESGK
                     GDKLRTADGRTLTDFCCSWGAMILGHAHDEVVEAIARQAARGTTFGINTPEEVTFAER
                     LTALVPGLERVRLVNSGTEAVMTALRIARGVTGRKRIIKFDGCYHGHSDSVLVGAGSG
                     LLTGGTLHSLGISRHVANDTFVAPFNDIDAVNEIISEKRVEIAAVIVEPVPGNMGLVM
                     PDPDFLVKLRRAADRCGALLIFDEVITGFRFGLSTYAQRVKVKPDLITLGKVIGGGLP
                     LAAVGGAAVFMDGLAPDGKIYQAGTLSGNPLALAAGLKTLEVLERDDPYPRMAQIGCR
                     MAEQINTAAAAKGLPLRCAQFQGVFTLFCNAEPVRNLTDAKRSDTQQYARLFHKLLQQ
                     GYYLPPSQFEVGFISAAHSEEAVMAFAQAVTDE"
     gene            1838..2674
                     /gene="panC"
                     /locus_tag="PLZ74_09115"
     CDS             1838..2674
                     /gene="panC"
                     /locus_tag="PLZ74_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOR98521.1"
                     /translation="MQVFTQPETMRQWAEEQRRAGRRIGLVPTMGALHAGHLSLVAEA
                     RRRADAVAVTIFVNPVQFGPNEDYAAYPRTETHDLACCREAGVDAVFLPSTTAMYAPD
                     ASVFVEEESLSLGLCGARRPGHFRGVCTVVAKLFHIALPHVAVFGQKDFQQAAVIRRM
                     VRDLDFPVEVVVAPIIREPDGLAMSSRNAYLTEEERQRAPGLSCALRQAAEAVAAGPC
                     DAETVRRNMTDVLVEHALKPDYVEIVDPDTLEPVAEARQGMVALVAAYCGKTRLIDNQ
                     VL"
     gene            2866..3684
                     /gene="larE"
                     /locus_tag="PLZ74_09120"
     CDS             2866..3684
                     /gene="larE"
                     /locus_tag="PLZ74_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOR98522.1"
                     /translation="MDEAVLTDKEQRLHVLLRQYGRVAVGFSGGVDSTLLLRVARDVL
                     GPEAVMAVIADTPSMPRSEFAEACALADAMGVVRVVTDSTELADPRYAENSSDRCYYC
                     KKHLFARVAALAAEKGFHAVLDGNNADDAGDYRPGRRASAELGVKSPLAEAGLTKEDI
                     RVLSRRLNLSTADKPAMACLASRIPYGSPVTVKALGQVECAEEALKAAGFKVCRVRHH
                     GEVARIELAASALSRLLDEPLREAVVAAVKAAGFRYVTLDLQGYRMGSLNASLP"
     gene            complement(4236..5066)
                     /locus_tag="PLZ74_09125"
     CDS             complement(4236..5066)
                     /locus_tag="PLZ74_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOR98523.1"
                     /translation="MSSLFQALECLAACGADGLLPDARNRLIRGLESCRTADGGFRSL
                     DRSCDPYYTFFGWLALRALRAPYDRNELCALMTPHTSAANPIDATCARLLLHVEKKSF
                     HTWLTSWIDLWRADDPYSVFLASLTFPLQPQWVMRLIWRAQRLQPDSLAPEGSPTPRL
                     AAGLLLTALAGRDTAMHAAALAARHHVTGGYASASGTQPDLLATAVARFALRVSANPE
                     KAPGLHAVHEADLAFIELCWLDDGLFGPSPTAEQGDTEHTFYGLLALGTCRPTPERIT
                     "
     gene            complement(5152..>5349)
                     /locus_tag="PLZ74_09130"
     CDS             complement(5152..>5349)
                     /locus_tag="PLZ74_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98524.1"
                     /translation="DAAKGVTDTLKGVTSGATDAAKGATSGTTGTADDAVKTAEGTVK
                     GAADAAKATTDKLKKLNPFGK"
BASE COUNT         1019 a         1479 c         1834 g         1017 t
ORIGIN      
        1 ctttgaactg gtcatagcgc ttttggaacg cgacgaactc ggttgtgcag accggggtga
       61 agtccgccgg atggctgaac agcacaaacc agctcccctt caaatcaccc ggaatgttca
      121 tcgggccttg tgtggtcttg accgacaact ccggaaactt atcgcccaac aacggcatgc
      181 tgttctttga catttccatg ttatgttacc tcccaactga gtgataaaac gtgtctgcca
      241 gatcacaccg catggcgtga acccgacccg aaggaacttc cttcaactgt cagattattc
      301 tgaaaaaaga ctcgggtgtc aacccccggc aatcggttgg cggggggcgg gaccgaaatc
      361 ggtatcggga ggcgaaatgc aatatcaact tccagtcccg atgccgccgt cccttttctt
      421 attgcaagtc actccttcat cgcgccctct aggttgaccg ttcgatgcga tattggtaat
      481 gtgattatgt gccttttaaa aaacgaaaac aggcgtgagg cgctacaaag tggatcacga
      541 agagttgtat acgcgggcgt gccgggtgat tccgggcggg gtcaacagtc cggttcgggc
      601 ttttaacgct gtgggagggg tgccgctcta tgtcgagtcc ggcaagggcg acaagctgcg
      661 gacggcggac ggacggacgt tgacggattt ctgttgttcg tggggcgcga tgatccttgg
      721 tcacgcgcac gacgaggtgg tggaggcgat cgcccggcag gccgcgcggg gcacgacgtt
      781 cggcatcaac accccggaag aggtgacgtt tgccgagcgc ctcacggctc tggtgccggg
      841 gctggagcgg gtgcgcctcg tgaactccgg taccgaggcg gtcatgaccg cgttgcgtat
      901 cgcgcgcggg gtgacgggcc gcaagcggat cattaagttc gacggctgtt atcacgggca
      961 cagtgacagt gtgctggtcg gtgcgggcag cggattgctt accggcggaa cgctccactc
     1021 gctcgggatc tcgcggcatg tggctaacga tacattcgtc gccccgttca acgacatcga
     1081 cgcggtcaat gaaatcatca gcgaaaagag ggtagagatc gccgccgtca ttgtggagcc
     1141 ggtgccgggt aacatggggc tcgtgatgcc ggatccggac tttctggtta aattgaggcg
     1201 ggcggcggat cggtgcggcg cgctgctcat tttcgatgag gtcatcactg gattccgctt
     1261 cggcctctcc acatacgcgc aacgcgtcaa ggtgaaaccc gaccttatca cgctgggcaa
     1321 ggtgatcggc ggtggcttgc cgctggcggc ggtgggcggg gccgcggttt tcatggacgg
     1381 gttggcgccg gacgggaaaa tctatcaggc gggaacgctc tccggaaatc cgctggcgct
     1441 ggccgcgggt ctgaaaacgc tggaggtgct cgaacgggat gatccctacc cgcggatggc
     1501 gcagatcggg tgtcgcatgg cggagcagat caacacggcg gcggccgcca aagggttgcc
     1561 cttgcgatgc gcgcagtttc agggcgtgtt cacgctcttc tgcaatgctg agccggtgcg
     1621 gaacctgacg gatgccaagc ggagcgatac gcaacagtat gcgcgacttt tccacaagct
     1681 gctgcaacag gggtattatc tgccgccctc gcaattcgag gtcgggttca tctcggccgc
     1741 gcacagcgag gaggcggtca tggcgttcgc gcaggcggtg accgacgagt gacggtggcg
     1801 gtccgcggct gttggctatg cggggaaggg aaagttgatg caggtgttca cacagccgga
     1861 aacgatgcgg cagtgggcgg aggaacagcg tcgtgccggc aggcggatcg ggcttgtgcc
     1921 gaccatgggg gcgttgcacg cgggacatct gtcgctggtc gcggaagcgc ggcggcgggc
     1981 ggatgccgtg gcggtcacga tttttgtgaa tccggtccag ttcgggccga atgaggacta
     2041 cgcggcctat ccgcgcaccg agacgcacga tctcgcgtgt tgccgggagg cgggggtcga
     2101 tgcggtgttt ctgccgtcga ctacggcgat gtatgcgccg gacgccagtg tgttcgtgga
     2161 ggaggagagc ctctccctcg gattgtgcgg tgcgcggcgt cccggacatt tccggggagt
     2221 gtgtaccgtg gtggcgaaac tgtttcacat cgcgctaccg catgtggcgg tgttcggtca
     2281 gaaggacttt cagcaggccg ccgtgatccg gcggatggtg cgcgatctgg attttccggt
     2341 cgaggtggtg gtcgcgccga tcatccgcga gccggacggt ttggcgatga gttcgcggaa
     2401 tgcgtacctg acggaagagg aacgacagcg tgcgcccgga ttgagttgcg cgctgcggca
     2461 ggcggcggaa gcggttgccg cagggccctg tgatgccgaa acggtgcggc ggaacatgac
     2521 ggacgtgttg gtggaacacg cgctgaagcc ggattacgtc gagatcgtag accccgatac
     2581 gctggagccg gtggcggaag cgcggcaggg aatggtggca ctggtcgcgg cctattgcgg
     2641 aaaaacgcgg ttgatcgaca atcaggtgct gtagacgcaa atacgggcgg agtccaaagc
     2701 caaggaggcg gggggagagg gaaacgggga atcggaacgc ccaatgtcga acgttgaaca
     2761 cggaacgccg aagcatcggt agggaggcat ccccgaggcg tccgcagatg ggacaatcgg
     2821 gatcgcacgc ttgaaacggc gaaccggaga acgggaacgc tgggtatgga cgaggcagtg
     2881 ctgacagaca aagaacaacg gcttcatgtg ttgctgcggc aatatggccg ggtggcggtc
     2941 ggattttcgg gtggtgtgga cagcacactc ttgttgcgcg tcgcgcggga tgtgctgggg
     3001 ccggaagcgg tgatggcggt gattgccgac acgccgagta tgccgcgcag cgagttcgcc
     3061 gaggcgtgcg cgctggccga cgcgatgggg gtggtgcgcg tggtgacgga ttcgacggag
     3121 ttggccgatc cccggtatgc cgaaaactct tcagaccgct gttattattg taagaagcac
     3181 ctttttgcgc gggttgccgc gctcgccgca gagaagggtt ttcacgcggt tctcgatggg
     3241 aacaacgcgg atgacgcggg cgattaccgg cccggtcggc gcgcgtcggc ggaactgggt
     3301 gtgaagagtc cgctggcgga ggcagggctg accaaagagg atatccgcgt gttgtcgcgt
     3361 cggctgaatc tgtccacagc ggacaaaccg gccatggcct gccttgcgtc acgcatcccg
     3421 tacggttcgc ccgtaacggt caaagcgctg ggacaggtgg aatgcgcaga ggaggcgctg
     3481 aaggctgcgg ggttcaaggt gtgccgtgtg cgccatcacg gtgaggtcgc acggatcgag
     3541 cttgcggcct ccgcgctatc ccggctgctg gatgagccgc ttcgcgaagc cgtggtggcg
     3601 gccgtgaaag cagcaggttt ccggtacgtg accttggatc tgcaaggtta ccgcatggga
     3661 agcctgaacg catcgctgcc ctgagccgag cacgatcaga atgcctgtcc gcaacagcca
     3721 gtgggtcgcc gccgcgtact cccacgactc gccgggcttg cggtacgcct caccgatgat
     3781 gaaccagttc catatttggg acatggcgcg ctcgaccgcg ttcggttctc gcggcacgga
     3841 tggcggtgcg gagtcggccg acagggcaga ctctgcgcac ggctgttgcg gcatgggagg
     3901 cggtacgggt tccggctctg catcgctgac ggacgcggcg gcgaccggtg cggactccgg
     3961 cgtggcgtcc gcgaccggta tgtcgggctt tgcggattca accaagcgtc cttccagccg
     4021 ggtgagttgc tgttgcagct cccgcatgcc gcggcacagg cctgaaacac gaatcagcag
     4081 gataaccgga atcaccagca agctcgcgac gattcccagt ccgatcatca ccacaaaaag
     4141 ctcaaccatt tcatccattg ctgaactcct tgaccatcac ggtttagaat cactttaggc
     4201 tgtctgcgtc tgtgcatcaa gcggaaagac gtgttttacg taattcgctc aggagtcgga
     4261 cggcacgtcc ccaaggcaag caggccgtaa aaagtgtgct cggtgtcacc ctgctcggca
     4321 gtgggagacg ggccgaatag gccgtcgtcc agccagcaca gttcgatgaa cgccagatcc
     4381 gcttcatgaa ccgcatgaag gcccggtgcc ttttcggggt tggcggatac cctcagcgcg
     4441 aaccgcgcta ccgctgtggc aagcaggtcg ggctgtgtgc cggaggcgga ggcgtatcct
     4501 ccggtaacat gatgccgtgc ggccaaggcg gcagcgtgca tggccgtgtc ccggcctgcc
     4561 agtgcggtca acagcagacc cgcggccagc cgcggtgtgg ggctcccctc cggcgcaagc
     4621 gaatcaggtt gaaggcgttg ggcgcgccag atcaatcgca tgacccattg cggttgaagc
     4681 gggaacgtca aactggcgag aaaaacgctg tagggatcgt ccgcacgcca gaggtcgatc
     4741 caactcgtca accaagtgtg aaagcttttc ttttcgacat gcaacaacaa tcgcgcacag
     4801 gtggcgtcta tcggattcgc ggcgctcgtg tgaggggtca tcaaggcgca aagttcattg
     4861 cggtcatacg gcgcgcgtaa cgcccgcagc gcgagccagc cgaaaaaggt gtaatacgga
     4921 tcgcaggatc ggtccaaact ccggaacccg ccgtcggccg tccgacaact ctccagaccg
     4981 cggatcagcc tgtttcgcgc atccggcagc aagccgtctg caccgcaggc ggcaagacat
     5041 tccagcgctt gaaagagcga agacatggag ataatcggag gggttctgaa acgaccaaaa
     5101 acctcttccg caggaacgga agaggttgca ttgcaagaaa accggaggcg cttatttacc
     5161 gaaggggttc agtttcttca gcttatcggt cgtggctttg gctgcgtccg ccgcgccttt
     5221 gactgtaccc tctgcggttt taaccgcgtc atccgccgta ccggtcgtgc cggaagtcgc
     5281 gccctttgcc gcatcggttg cgccagaggt cacgcccttc agtgtgtcgg tcacgccttt
     5341 tgccgcatc
//
LOCUS       DAOSRB010000186         5345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_14009_length_5345_cov_7.726087, whole genome shotgun sequence.
ACCESSION   DAOSRB010000186 DAOSRB010000000
VERSION     DAOSRB010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14009_length_5345_cov_7.726087"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..3561)
                     /locus_tag="PLZ74_09135"
     CDS             complement(94..3561)
                     /locus_tag="PLZ74_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98525.1"
                     /translation="MRRLMGMGALAVMVAGGAYGATPPSAASDETGRLRLYAFGDLIG
                     NAVSNAAGPGNAMLYKAASGAVPVTGPGRRAGSRAVVLDADVFSAPAVTQASNAFTVA
                     LWLRPQAAGSKKAGSRSNGMIASNGSGYYDGWRLLLHDWRTRCPTIELGRGKDKGALG
                     VHARDSLAVGFWNHLAATWDGARVRLYVNGMLSAETPYAGPLVPPRGALCAGFSGYGV
                     GSLGMAIDTLAVYDRALPPETVARIALDGEALPADCVALLGQAQAAAGAGARSEASQA
                     YRALAGKGEAPAIWRQWAALAALRFIETAADRRAGVEACAAFYSDASVPETLRGQAIE
                     FLAQICRRGLGGEVLPSRILADLPEHLELDAGDPRLFGLALAEAYQRERQFDAAYGVY
                     RQLLTAAGDEAEAAAGIRQGYARALWQGGCREEARAQYAALAADTRLPESARGLAALA
                     VAQVWREEGKYAEAAAAYRAVTGTVSRFTHLLDEARVGAEACANLLEGKPARDPEASR
                     QRLAPLPEPAVRFVVSPKGRDTNPGTFEQPFATLERARDAVRAAKRGGSLPPGGACVF
                     LRGGRYAVTNTFVLEKGDSGSFGAPVVYCAWPGERPVFDGGFRVRGFWRVRDAGTLKR
                     LPEEARGHVHVANLTKQGYTALEAQASYGRAQTNETVRELFQDGQPLEIARWPNDGFL
                     KMATANITNLTFSCPTNRLARWTQADDLMANGYWFHLWAECTVPLAAVDAATGVFTFR
                     QKPDHNLRAGYPFYVLNLLEEIDRPGEWFLDRAAGRLYVWPLKQPWRSTLTLSQWNRP
                     FVEARKVQEVVFRGLTFEYGRQNGMVLDACANVTVAGNVIRRLGGTALIVTQGANIRI
                     YGNRMETLGHAGMRVEGGNRTNLTSGRIVIENNEVSHFARCSRTYNPALLLDGCGARV
                     AHNHFHHAPSSAMRIEGNDHLIEYNRVEHMVRESDDQGGIDMWCNPSYRGVVIRYNLW
                     RDIGGGHAPCGQAGIRFDDAISGMVVYGNRFERTSTGHFGGVQIHGGQDNIIDNNLFT
                     ACRYGVSFSAWGQKRWEDFLGRERIRNLMYSDVNITLPPYSKRYPELAGLPGKADVNS
                     IWRNLFIGTEEALHKKPKGTETWENRVFAEAPDLEEVAACSPFETLPPEDEIGLYADP
                     FRAAASRDF"
     gene            complement(3704..4240)
                     /locus_tag="PLZ74_09140"
     CDS             complement(3704..4240)
                     /locus_tag="PLZ74_09140"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOR98526.1"
                     /translation="MKKRKLPLSKVYTLIEPGPVVLVTTMDQGRPNVMTLAWHTMLDF
                     EPPLLGMVLSEQNHSFAVLRKTKACVINLPSVALRKAVVGCGNTSGRDTDKFAAFGLT
                     PRPAKQVAAPLIDECFASLECVVTDTRMVRQYNLFVLEVVQAWIDPDCGQPKTLHHCG
                     TGNFMVAGRRITLPSAMK"
     gene            complement(4369..5121)
                     /locus_tag="PLZ74_09145"
     CDS             complement(4369..5121)
                     /locus_tag="PLZ74_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOR98527.1"
                     /translation="MRYRKQILAPCFGEATQHCLAKATVAIVGCGGLGTWQAELLARM
                     GIGRLRLADDDRVTLENLHRQILFTEQDAVAGLLKVRAAAARLRQINTHVVIEPYEMR
                     ITRDTLDAFAAEADLVLDATDHAPTRFLINDFCLAHAIPWIYTGVAGEEGLILPVLPS
                     GGPCLRCLYPDPPEPDETANCRTDGILPMTVAMAVSLQIAQAVRVLNGNAEPGTLIRF
                     NVWSAVARRVRLERLPTCPGCGLRLRNGTNET"
BASE COUNT          979 a         1867 c         1568 g          931 t
ORIGIN      
        1 agcctcacag cctcacagcc tttgagattg cgggctccgc ccgcatctca atacggtgcg
       61 tcgggcgcac cgcaaaaccc aatgtttacc gtcctagaaa tcccgtgatg cggccgcacg
      121 gaagggatcc gcatacaggc cgatctcgtc ttcgggcggc agcgtctcga aaggtgaaca
      181 ggccgcgacc tcttccaaat cgggggcttc ggcgaacacg cggttctccc aggtctcggt
      241 tcccttaggc tttttgtgca gcgcctcttc ggtcccgatg aaaaggttgc gccagatgct
      301 gttgacatcc gctttgccgg gcagcccggc cagttcggga taccgtttgc tatagggcgg
      361 cagcgtgatg ttgacgtctg aatacatcag gttccggatt ctctcgcggc ccagaaagtc
      421 ctcccagcgc ttctggcccc atgccgagaa actcaccccg taccggcacg cggtgaagag
      481 gttgttgtcg atgatgttat cctgcccgcc gtggatctgc acgccgccga aatgccccgt
      541 cgaagtccgc tcgaagcggt tgccgtaaac gaccatgccg ctgatcgcat cgtcaaaccg
      601 gatgcccgcc tgaccgcacg gcgcatggcc tccgccgatg tcccgccaca gattgtaacg
      661 gatcaccacg ccgcggtagg acggattgca ccacatgtcg atcccgccct gatcgtccga
      721 ctcccgcacc atatgctcca cgcggttgta ctcgatcagg tggtcgttgc cctcgatccg
      781 catggcgctg gacggcgcgt ggtgaaaatg attgtgcgcc acgcgcgcgc cgcagccgtc
      841 gagcagcagc gcagggttgt aggtacgcga acagcgggcg aaatgggaga cttcgttgtt
      901 ttcgatcacg atgcgtcctg aggtcaggtt ggtacggtta ccgccctcga cccgcatgcc
      961 cgcatgcccg agcgtctcca tccggtttcc gtaaatccgg atgttcgcgc cttgggtgac
     1021 gatcagcgcc gtgccgccca aacgccggat cacattcccc gccacggtca cgttggcgca
     1081 ggcgtccagc accatcccgt tctgccgccc gtactcgaag gtcaggccgc ggaacaccac
     1141 ctcctgcacc ttgcgggcct cgacaaacgg acggttccat tgcgacagcg tcagcgtgct
     1201 gcgccaaggt tgtttgagcg gccacacgta gagccgtccc gcggcccggt cgagaaacca
     1261 ctcgccgggc cggtcgattt cctccagcag gttcagcacg taaaagggat agcccgcacg
     1321 caggttgtga tccggcttct ggcgaaacgt aaagactcct gtcgcggcat cgaccgcagc
     1381 caagggcacc gtgcactcgg cccacaggtg gaaccagtag ccgttcgcca tcaggtcatc
     1441 cgcctgcgtc cagcgcgcca accggttggt cggacaggag aaagtcaggt tggtgatgtt
     1501 ggcggtcgcc atcttgagga agccatcgtt gggccaccgg gcgatctcca gcggctggcc
     1561 gtcctgaaac agctcgcgaa ccgtttcgtt ggtctgagcc ctcccgtacg aggcctgcgc
     1621 ctccaacgcg gtataccctt gtttcgtcag gttcgcgacg tgaacatggc cgcgcgcctc
     1681 ctcgggcagc cgcttcaacg tgccggcatc gcgcacgcgc cagaatcccc gcacgcggaa
     1741 cccgccgtcg aacaccggcc gttcgccggg ccaggcgcaa tacactaccg gcgctccaaa
     1801 actgccggaa tccccttttt cgaggacgaa tgtgttggtc accgcatagc ggccgccgcg
     1861 cagaaacacg caggcgccgc ccggcggcag actgcccccc cgtttggcgg cgcgcaccgc
     1921 gtcgcgcgcg cgttccagcg tggcgaacgg ctgctcgaag gtgccggggt tcgtgtcccg
     1981 gcctttgggc gacacaacga aacgcaccgc cggttcgggc agcggggcga gacgctgacg
     2041 gctggcctcg ggatcgcgcg caggcttacc ctccagcagg ttagcgcacg cctccgcgcc
     2101 aacacgcgcc tcatccagca gatgcgtaaa ccggctgact gtccctgtca ccgcgcggta
     2161 tgccgcggcg gcttcggcgt acttgccctc ctcgcgccat acctgcgcca ccgccagcgc
     2221 cgcgagtccg cgcgcgcttt ccggcagacg cgtatccgcc gccagcgcgg catactgtgc
     2281 gcgcgcctcc tcccggcaac cgccctgcca gagtgctcgg gcataaccct gccggattcc
     2341 ggcagccgcc tccgcctcat cccccgccgc agtgaggagt tgccggtaca caccgtacgc
     2401 cgcatcaaac tgccgctcgc gctgataggc ctccgcaagg gcgagaccga acaggcgcgg
     2461 gtccccggca tccagttcga gatgctccgg cagatcggcc agaatccggc tggggagcac
     2521 ctcgccaccc agtcctctgc gacagatctg cgccagaaat tcaatcgcct gaccccgcag
     2581 ggtttcaggc acggacgcgt cggagtaaaa cgccgcgcac gcctccaccc ccgcacggcg
     2641 atcagccgcc gtttcaatga accgtagcgc ggcgagtgcg gcccattgcc gccagatggc
     2701 tggcgcctca cccttcccgg ccaacgcccg atacgcctgc gacgcctcgc tccttgcgcc
     2761 cgcgcctgcc gccgcctgcg cctgacccag caacgccacg cagtcggcgg gcagcgcctc
     2821 accgtccagc gcgatccggg cgaccgtctc gggcggcagt gcgcggtcat acaccgcaag
     2881 cgtgtcgatc gccatgccga gcgagccgac cccgtaaccg gagaaccccg cgcagagcgc
     2941 gccacggggc ggaacgagcg ggcccgcata cggcgtttcc gccgacaaca tcccgttgac
     3001 atacaggcgc acacgcgcac catcccaagt cgccgccaga tgattccaga agcctacggc
     3061 caatgagtcc cgcgcgtgca cgcccaacgc gcccttatct tttccccttc ccaattcgat
     3121 cgtcgggcaa cgcgtccgcc aatcgtgaag gagcagacgc cacccgtcat aataaccgct
     3181 gccattcgag gcgatcatcc cgttcgagcg gcttcccgct tttttcgagc ccgccgcctg
     3241 cggccgcaac caaagcgcca ccgtgaacgc gttcgatgcc tgcgtcacag ccggggcaga
     3301 gaagacatcc gcgtcgagca ccactgcccg cgaaccggcc cgtcgtcccg gccccgtgac
     3361 cggtaccgcg cccgacgccg ctttatacag catggcgtta cccggccccg ccgcattgga
     3421 gaccgcatta ccgatcaggt ctccgaacgc gtacagccgt aaccgtcccg tttcatcgct
     3481 tgcggcagac ggcggcgttg ccccatatgc gccgcccgca accatgaccg ccaacgcccc
     3541 catccccatc aatcgtctca cctgcttatt catgcctcca gtgtaaacga cccgtcccgg
     3601 atgttcaaga cgggctcgtt cccgatgccg caaaagattc tttatgcgct tggtgtcaga
     3661 ttgggttgcg gtgcggactg tacccgcatt cgtgagcccc ggatcacttc atcgccgacg
     3721 gcagcgtgat gcgccgcccg gccaccataa agtttcccgt accgcagtgg tgcagtgttt
     3781 tcggctggcc gcagtccgga tcaatccacg cctgcacgac ctccagcaca aacaggttat
     3841 actgccgcac catacgggtg tcggtgacca cacattcgag cgaagcgaag cactcgtcga
     3901 tcaatggggc cgcgacctgt ttcgccggac gcggcgtgag gccgaacgcc gcgaatttat
     3961 ccgtgtcgcg acccgaggtg ttgccgcatc caaccaccgc cttgcgcaga gcgactgagg
     4021 gaagattgat cacacaggcc ttggtcttac gcaatacggc gaacgagtgg ttctgttcgc
     4081 tgagcaccat gccgagcagc ggcggctcga aatccagcat cgtgtgccaa gcaagggtca
     4141 tcacattcgg acgcccttga tccatcgtcg tcaccagcac aaccggcccg ggctcaatga
     4201 gggtgtagac tttggagagc ggcaactttc tttttttcat gtccccagtg taaacgaccc
     4261 gtcccggatg tgcaaggttg ggaaaaaagg aagagcgttc aacgtccaag ggtcgaatcg
     4321 aaaatcttaa cacgcggagt gaaaaaatca gcggatcggt cagaaacgtc acgtctcgtt
     4381 cgtcccgttc cgcaggcgga ggccgcatcc gggacaggtg ggcaggcgtt cgagccggac
     4441 acgccgtgcc accgcagacc agacattgaa ccggatgagc gtgccgggct ccgcgtttcc
     4501 gttcaacacg cgcaccgcct gggcgatctg cagggagacc gccatggcaa cggtcatggg
     4561 cagaatgccg tccgtgcggc agttggcggt ctcatcaggt tcaggcggat cgggatagag
     4621 gcagcgcaga cagggcccgc cggacggcag caccggcagg atcagccctt cctcgcccgc
     4681 cacacccgtg taaatccatg ggatcgcgtg ggccaggcag aaatcattga tcagaaagcg
     4741 cgtgggcgca tgatccgtgg catccagcac cagatcggcc tccgcggcaa aggcatcgag
     4801 cgtgtcgcgc gtgatccgca tctcatatgg ttcgatcacc acatgggtgt tgatctgccg
     4861 caagcgcgcg gcagcggcgc gaactttcag gagacccgcc accgcatcct gctccgtgaa
     4921 caggatctgc cggtgcaggt tttcgagcgt cacgcgatcg tcatccgcca gacgcaaccg
     4981 tccgatgccc atgcgcgcca gcaactccgc ctgccaagtg ccgagtccac cgcatcccac
     5041 aatggctacg gtggcctttg cgagacagtg ctgggtcgcc tctccgaaac acggcgccaa
     5101 gatctgtttc cggtatcgca tcttcccctc ccccatgccg cgtctccggc gcgtccgggt
     5161 gatccggccg cccgcgtgtc cgcatcacgg gaaagcatac caacgaaaca cgccgtcgtc
     5221 caccccgtca cgaaccgaga gcgtgtctct accgtagccc ttaaaagcgc gttgaaaatt
     5281 caactctctt gggtttcagg gtttcatggt ttcggggttt cagggtttca gggtttcatc
     5341 caaat
//
LOCUS       DAOSRB010000187         5310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_14138_length_5310_cov_6.837488, whole genome shotgun sequence.
ACCESSION   DAOSRB010000187 DAOSRB010000000
VERSION     DAOSRB010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14138_length_5310_cov_6.837488"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            606..4922
                     /locus_tag="PLZ74_09150"
     CDS             606..4922
                     /locus_tag="PLZ74_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98528.1"
                     /translation="MAPRASAIQGNQPFRIDSTEYGGHIFNMQGYDDTADAVLRFENL
                     LLEAWTTSEPKRTTIDVRGGLMDFRRPMDGVSTVKSWLQLGNNVGGNELVYRIGPGAT
                     NRFSTADLAMSAPQKGYIVVDGGLLEVDGLLALNGNNTLCKEFVITNGGVVTANQLFL
                     GNVTGGSRWRVDVVNGSTLAAAGTFKQCSFTGSLFDINIGAHSRLILSKQDQTYFGNV
                     EGATLNVNLFDGGTLLVTNDFDFTMKNQTRPVNGGAFLNVSNGTLRIAPTGIFRMAQN
                     LYGTHNFTIRNEGKLFIGHSRPDGDLTTWDLSSAHFDFTDDSVLYIGCYGNDGAHVII
                     TNGTNTIRGKEFVLGKDGSPNSALTIGGNADITFGPITDKAVFNHLDRGDGTTFNLIG
                     GRTTWLEKPTFGSKNVGRLTVNVTGGVHAFNTAVSIGYFGLCDWFQSGGDVTVKDSLT
                     VGNWPGFPEAHRPQYFRLTGGSLTTLENLVVADGGNRAAIVELNGGILKAKRVYGWNG
                     ATVRKPDAGGLAILHGDGGTLEALSAQNAFMVFLDEAKVGTTGLVIRTDFDITLCAPW
                     ADLDGAEGEGRLILCGPGKKTFVKNLVDANLTCSNGALTNGITVCAGGLVHFADELPI
                     TTKLVVTNNAMVSFAGTSTGATLKGLVLGDATSGGTLRLDPGDHFTVDGPVSFLNAAL
                     EFTSPPEPADYVFLTVSQGVPVDAATKTAWEQMLIAAGHGKGKTYSFSVTEDESGNQI
                     FGLKVETAEPLADTTTWQGPGTAWETNENWSAMSPGSHAVAVFSSDTAPAAVTLDTPV
                     TIGALCFTNAGTSIGGAGGIGFTDSGYAEVSAEAGLHEIGVGLLPTFELPFALAEGSR
                     VRIAGPITGGAINKTGEGILALANENNSLRGAIRIDGGAVVAESIATLGMRNGGANKV
                     TLKKGVFAISNDVADATLPQTFELDAGAKTSAVMRVDEDLTVSAFSCASGNLVKRGRG
                     RLTLAYGTGTVHLARDDGYSARSFSVNEIVYKENGSVPDDLTGFSGINVIEGELVLKG
                     TAGKPEDTIFWLHNVDWRIGQNTATVADPIFTLDHAKLYNSDSHCALGYGVNVTGGTK
                     TASLNLRNGAWFYGNSIFSGSNASGMTGAPEDDVYCRIHVSGASTLETEYILLLSSAH
                     KGWGEHLFEEGSEVLARLGVGVEVRYPVRATWDDGSILAATRNNGVYTPTRLNVKDGG
                     RGSFTFQNGSTLYANLFTHNCNAAFAFVFDDGTWDACGDMDFCFRYASKVSIETTGKG
                     LTLPPADKQTYRMAAKISGTGGVVKTGAGTLAFVPHETLDAATGTTPEPLVDDPVTWD
                     FDGTLDIREGVVTVANGCTRSGLLPVKVASEAELRLASGSDVTLSMDLGYSEDAPMPE
                     SLLGKTVALPITYDGDAPTKLADWEVGPVGIPQTTVRLLAENGGIFLLVERDVGFRLI
                     LK"
     gene            complement(5112..>5310)
                     /locus_tag="PLZ74_09155"
     CDS             complement(5112..>5310)
                     /locus_tag="PLZ74_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98529.1"
                     /translation="RSSRVECRGSRVDDPDSDSDTDTDPDPDLDETQKPETLKPETLK
                     PKSVAFNALFRFTHPRGVRSR"
BASE COUNT         1097 a         1508 c         1619 g         1086 t
ORIGIN      
        1 cttgttgcgg ccacgcagtg gctcgcaacc gtgagagtaa aagcgctgct cggttgtttc
       61 gcgcaaggcg gcacccgcat tggtttcatg gcacatatag cgtgactttt cggctggaac
      121 cgctccctcc atattggtat ggtatgccag aatacaatga ttgacggtcc ggtatcggaa
      181 atggggccgc gtgttgtgca tcgaaacgca ggacgaatac gaaatgacga gcaggaatga
      241 cgctgatagc catgacgtca gatagatggc aggtgatcac gccacacaag aaagggaaaa
      301 ccatgttcag gcaaatcgct tttttggtaa tcacgatcgt atgggccagc tcctcggcag
      361 cgttgacgga tgtcaaatgg gcctcgtcca acggacaacc cgtttctggg tccctgctcg
      421 atgatgccag ttgggaaggg ggctcagcgc ctgtcccggg aacagggatc ggcaagttat
      481 atgtccaaag cgggccttac gcagtcaccc tccctggggc gacggcaagt ttcccgttct
      541 gggtgcgcgt gccatccgcc gacgtcggca aggtaacgct agacggccgc ggcaattgtt
      601 tcatcatggc accgcgcgcg agcgctatcc agggaaacca gccgtttcgc atcgattcga
      661 cggaatacgg cgggcacatc ttcaacatgc agggctacga cgatactgct gacgccgtat
      721 tgcggttcga gaatctgttg ttggaggcat ggaccacatc cgaacccaaa cggacaacca
      781 tcgacgtccg cgggggcctc atggatttta gacggccaat ggacggtgtg tcgacggtca
      841 agtcgtggct gcagctcggc aacaatgtcg gcggcaatga actcgtctat cgtatcggac
      901 cgggcgcgac gaaccggttc tccaccgcag atctggcgat gagcgcaccc caaaaaggtt
      961 acattgtcgt cgacggcggc ttgctcgaag tcgacgggtt gctggcactc aacgggaaca
     1021 acacactctg caaggagttt gtgatcacca acggcggtgt ggtgacagcg aaccagctct
     1081 ttttaggcaa tgtgaccggt ggcagccgct ggcgcgtgga tgttgtcaac ggttcaacgc
     1141 tcgccgcagc cgggactttc aaacagtgtt cgttcacagg cagcctgttc gacatcaaca
     1201 tcggagccca ttcgcggttg atcctcagca agcaggatca aacttacttc ggcaatgttg
     1261 aaggggcgac gctgaacgtg aatcttttcg atggaggcac acttctcgtc acgaatgatt
     1321 ttgacttcac catgaaaaat cagactcggc cggtgaacgg cggtgcgttt ctgaatgttt
     1381 ccaacggcac attacgcatt gctccgaccg gcatcttccg gatggcccag aacttatacg
     1441 gaacccataa tttcacaatc cgcaatgagg gcaagctttt catcggacat tcgcgtccgg
     1501 atggggatct gacaacttgg gacctttcgt ccgcacactt cgatttcacc gatgattcgg
     1561 tcctctacat cggctgttac ggtaacgatg gcgcgcacgt catcatcacc aacggcacga
     1621 acacgatccg cggcaaggaa ttcgtcctcg gcaaggacgg ctcgccaaac tcggcactca
     1681 cgatcggcgg caatgccgac atcacctttg gaccgatcac cgacaaagcc gtcttcaatc
     1741 acctcgaccg cggggacgga accacgttca atctgattgg cggacggacg acatggctgg
     1801 agaagccgac gttcgggagc aagaacgttg gacggctgac tgtgaatgtc acgggcgggg
     1861 tccatgcgtt caacacagcg gtgagcattg gttactttgg tctctgcgac tggttccagt
     1921 ctggcggcga tgtcaccgtt aaggatagtt tgacggttgg aaactggccc ggattccccg
     1981 aggcgcatcg gccccagtat ttccgtttga ccggcggctc gctcacgacg ctcgagaacc
     2041 tcgtggtggc ggacggcggc aaccgtgcgg cgatcgtcga actcaacggc ggcatactca
     2101 aggcgaaacg cgtctatggg tggaacggcg cgacggtgcg taaaccggat gcaggcggtc
     2161 tcgcgattct gcatggcgat ggcggtacgc ttgaggctct ttccgctcaa aatgcgttca
     2221 tggtctttct cgacgaggcg aaggtcggca cgaccggtct ggtcatccgc acggactttg
     2281 acatcacgct ctgcgcaccc tgggccgatc tcgatggggc ggaaggcgaa ggtcgcctga
     2341 tcttgtgcgg gcccggcaag aagacattcg tgaagaatct ggtggacgcg aatttaacgt
     2401 gctcaaacgg tgcgcttacg aacggcatca cggtctgcgc gggcgggctc gtccacttcg
     2461 cggacgagct tcccattacg acgaagctcg tggtcacgaa caacgcgatg gtctccttcg
     2521 caggcacgtc gacgggcgca acattaaaag ggcttgtcct cggtgacgcg acgagtggcg
     2581 gcacgctgcg gcttgatcct ggcgatcact tcacggtcga cggcccggtc tcgttcctga
     2641 atgcggcgct tgaattcaca tccccgccag agccggcgga ctacgtgttt ctcacggttt
     2701 ctcagggcgt tccggtcgat gcggcgacga agacggcctg ggaacagatg ctcattgccg
     2761 caggacatgg caagggcaag acgtattcct tctcggtaac ggaggacgag tcaggcaatc
     2821 agatcttcgg gctcaaggtc gaaacagctg aaccgcttgc cgacacaacg acgtggcaag
     2881 gccccggcac ggcttgggag accaacgaaa actggtccgc catgagcccg ggttcccacg
     2941 ctgtggccgt gttctcctcc gatacggctc cggcggcggt aacactcgat acgccggtca
     3001 caatcggtgc cctatgcttc acgaacgcgg gaacgtcgat cggcggcgca ggcggcatcg
     3061 gcttcaccga ttcgggttat gcggaagtgt ccgccgaagc gggtttgcat gaaatcggtg
     3121 tcggcctttt gccgaccttc gaattgccgt ttgcgcttgc cgagggctcg cgcgtccgca
     3181 tagcaggacc catcacggga ggcgcgatca ataagacagg cgaaggcatc ctcgctctcg
     3241 ctaatgagaa caattccctg cgtggcgcga ttcgcatcga cggcggcgcc gttgtggcgg
     3301 aatcgatcgc gacgcttggc atgcggaacg gcggtgcaaa caaggtgacg cttaaaaagg
     3361 gtgtcttcgc catctcaaac gacgtcgcgg acgcgacgct gccgcagacg tttgaactcg
     3421 atgctggagc gaagacgtcg gctgtgatgc gcgtggatga agacctcacg gtttccgcct
     3481 tctcgtgcgc ttccggtaac ctcgtcaaac gcggccgcgg acgactcacg ctcgcctacg
     3541 ggacaggaac ggttcatctc gcacgagatg acggctattc cgcacggtcg ttctccgtga
     3601 acgaaatcgt ctacaaagag aacgggtccg ttccagatga tctcacgggg ttctccggca
     3661 tcaacgttat cgaaggcgaa ctggtgctca agggaacagc cgggaagccc gaagacacca
     3721 tcttctggct ccacaacgtc gattggcgca tcggccagaa cacagccacc gtggcggacc
     3781 cgatctttac cctcgaccat gccaaacttt ataactcgga ttcgcattgc gcgctgggtt
     3841 atggcgtcaa cgtaaccggc gggacaaaga ccgcctcgct caacctgcgc aacggcgcgt
     3901 ggttctacgg gaattcgatc ttttcgggaa gcaatgcatc cggaatgacc ggcgcacccg
     3961 aggatgacgt ctactgccgg atccatgtgt ccggcgcgtc gacgctcgaa accgaataca
     4021 ttctgttact ctcgtccgca cacaagggct ggggtgagca tctttttgaa gaaggttcgg
     4081 aggtcttggc tcggttgggc gtcggcgtgg aggtccgcta tcctgtccgt gcgacatggg
     4141 acgatggatc cattctcgcc gcgacgcgca acaacggcgt ttatacgccg actcggctca
     4201 acgtgaagga cggcggtcgc ggttcgttca cgttccagaa cggttcgaca ttgtatgcca
     4261 atcttttcac ccacaactgc aatgccgcgt ttgcgttcgt gttcgacgac ggcacatggg
     4321 atgcctgcgg ggatatggac ttctgtttcc gctacgcgtc caaggtgtcc atcgagacaa
     4381 ccggaaaagg cctcacgctg ccgccagccg acaaacaaac ctaccggatg gcggcgaaga
     4441 tcagcggaac gggcggcgtc gtgaagaccg gtgccggcac gcttgcgttc gtgccgcacg
     4501 agacgctgga cgctgcaacg ggcacgacgc ccgagccgtt agtcgatgat cccgtgacct
     4561 gggatttcga cgggacgctg gacatccgcg aaggcgttgt caccgtcgcg aacggctgca
     4621 cgcgttcggg cctgttgccc gtgaaggtgg cgtccgaagc cgaactgcgt ttggcatccg
     4681 gttccgacgt gacgctatcc atggatctgg gatattcgga ggacgcgccg atgccggaga
     4741 gtctgctggg caaaacggtg gcgttaccca tcacctacga tggcgacgcg ccgacaaaac
     4801 tggcggattg ggaagtcggt ccggttggaa tccctcaaac gacggttcgc ttgttggctg
     4861 aaaacggcgg catattcctg cttgtcgaac gcgatgttgg gttcaggctg atccttaaat
     4921 aagcaaatca ggatagattg ccgagtggac tgcaaatgcc actttgtcgc gtgcggcacc
     4981 acggatgtgt cttcggtggg gacgcgggcg tgcgttccgc gaaaggagcc ggaaaatgat
     5041 ccatttcttt tgcatggcag cattgacgtt gaccgcacct gctggccgtt tccgcaaacc
     5101 gccgccggtc cctaccttga gcggacgcct cggggatgcg tgaacctaaa aagagcgttg
     5161 aaagcaacgc tctttggttt cagggtttct ggtttcaggg tttctggttt ctgggtttca
     5221 tccaaatcgg ggtcggggtc ggtatcggta tcgctatcgc tatcgggatc gtcgacccgc
     5281 gacccgcgac attcgacccg cgacgatcga
//
LOCUS       DAOSRB010000188         5223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_14467_length_5223_cov_5.760255, whole genome shotgun sequence.
ACCESSION   DAOSRB010000188 DAOSRB010000000
VERSION     DAOSRB010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14467_length_5223_cov_5.760255"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLZ74_09160"
     CDS             <1..386
                     /locus_tag="PLZ74_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98530.1"
                     /translation="RYTVRREVQCLISACLMIPAALLREHGGFDEAFHPVQFEDFDLV
                     YRLREAGHRAFVEPAAEMYHFESVTTQGTPTVRNAAAVVRNGLLFQKRWRHRFEQEEG
                     PPEEACRWRRIEPKPFDEIVDPPLF"
     gene            complement(429..1421)
                     /locus_tag="PLZ74_09165"
     CDS             complement(429..1421)
                     /locus_tag="PLZ74_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOR98531.1"
                     /translation="MARPVTIFTGQWADMPFEKVCELMSGFGYEGLEVCTWGDHIDLD
                     QASRSKTYCDEKRGLMEKYGLGCWAISNHLSGQLVCDPIDARHYAFAPAKVRNDPKAA
                     RKWAIDMQKKAAKAARNMGVTVVNGFTGSPIWHLLYSFPPCLPGQIESGYREFAKTWK
                     PILDVFADNGIRFALEVHPTEIAFDIASAQRALEALDGHPAFGFNFDPSHLGYQGVDY
                     VKFIRTFPDRIFHVHMKDAWWGHGDGTVGVFGGHTEFADPRRFWDFRSLGHGDINFED
                     IIVALNDIGYQGPLSVEWEDSRMDRCHGAQESCDFVKGVDFPPSAIAFDAQFDR"
     gene            1660..2682
                     /locus_tag="PLZ74_09170"
     CDS             1660..2682
                     /locus_tag="PLZ74_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOR98532.1"
                     /translation="MLNKLLSFFSSDIGIDLGTANTLVYVKDRGVVIREPSVVAIQEG
                     TKRILAVGEEAKRMLGRTPANIVAIRPMQSGVIADFDITEAMIRYFIRKVHVAKVVKP
                     RVIVAVPSNITEVEKRAVQESARHAGAREVFLIEEPMAAAIGVGLPVSEPAGNMIVDI
                     GGGTCEVALISLAGIVYARSVRVGGDAMDECIVQYMRRVYNLMIGERTAEEIKITIGS
                     AYPLGEESTLEVKGRDLVAGLPKTLTVTSEEIRDALQEPVSSIVDTIRITLEKCPPEL
                     SADLVDRGLVLAGGGAMLRGLDKLIASQTGLPVTLADDPLSAVAEGTGVVLNELNFLA
                     KTKLGR"
     gene            2741..3592
                     /gene="mreC"
                     /locus_tag="PLZ74_09175"
     CDS             2741..3592
                     /gene="mreC"
                     /locus_tag="PLZ74_09175"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOR98533.1"
                     /translation="MKQTKLWVGLLAVTLAALVFWRTGAHDFVREALYPYQKACVWFE
                     RAVWLRARAMFSRSRLAARNAMLERDVARLRMVADDVEALEAETRRLRALLDFVPPVP
                     CRWLAAPILSRGGTSAVWQQVRLGKGSLHGVRKGAPVVVPDGVVGRVMDVSLHTSDVM
                     LITDPNSRVACELETPGEGVGVVRGILYGGGSVPAAGPELTLLYVVEPLRLRYLARDF
                     EPSPHTRVVTSGLGQTFPKGLTVGFLLDSRIEPNALSREAAVMPAVDFAALDEVFVLS
                     PGGTHAP"
     gene            3582..4121
                     /locus_tag="PLZ74_09180"
     CDS             3582..4121
                     /locus_tag="PLZ74_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98534.1"
                     /translation="MRLDTDLFRIFFALGVAALLQEMFPVIPLVPAKPGFLTAVALYY
                     ALTRPWPVALTALTWAGALTDLQDGLPLFCTIGFLLVAYGAVRLMQRLFLTATVAHGA
                     VLVAGMAAAQTLWTRLCVGVGGWSLLWQTLAAAGYAAHAGLVAGGLGFGFCGLLDRVS
                     GSERPVKEDNGIGWTETDQ"
     gene            4090..>5223
                     /locus_tag="PLZ74_09185"
     CDS             4090..>5223
                     /locus_tag="PLZ74_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOR98535.1"
                     /translation="MESGGQKQTSSWRIALLALVFAAGVAVLGSGLYRVQVVQNPSFS
                     RDQLRQSVRRVQEPGLRGRIFDRNGICLAENRASYCIAFYVEELRKRGPWIRTIDAVN
                     TEIDRLAAVLGVPRVISVAAVSNHVINSRYMPLLAWRDVGQEILARWAETQEVFPGVD
                     VYVQPERSYPQGALAAHVLGYVKRDMPLPLPGERVHFFLPEMFGANGVERQYNALLTG
                     RSGGKLIRVDARGYKHAVYQGEEAVAGQDLHLTLDVRIQRTLEQALRGLRGAGVVLDP
                     RNGEVLAMASAPAYDANAFAPYISTGLWRSLNTDPHLPLFNRALQGGYAPGSTFKPVT
                     AIAALIHGAVEPETLHTCTGVFELGTMRLHCWNTYGHGAIALRK"
BASE COUNT          900 a         1445 c         1819 g         1059 t
ORIGIN      
        1 cgcgttacac ggtgcggcgt gaagtgcagt gcctgatcag cgcctgcctg atgatcccgg
       61 cggcgctgct ccgcgaacac ggcggcttcg acgaggcgtt ccatcccgtg cagttcgagg
      121 atttcgatct ggtctaccgg ttgcgcgagg ccgggcatcg cgcctttgtg gagcctgcgg
      181 cggagatgta ccatttcgag agcgtcacca cgcaaggcac gcccacggtg cgcaatgcgg
      241 cggcggtggt gcgaaacggg ctgctcttcc agaaacgctg gcggcaccgg ttcgagcaag
      301 aggaaggccc tccggaggaa gcgtgccgct ggcgccggat cgaaccgaag ccctttgacg
      361 agatcgttga cccgccgcta ttctaataaa aaagagcagt tgaattttca actgctcttt
      421 ttttaggttc agcggtcgaa ttgcgcgtcg aacgcgatcg cgctgggcgg gaagtccacg
      481 cctttgacaa agtcgcagga ctcctgggca ccgtggcagc ggtccatgcg ggaatcctcc
      541 cactcgaccg agagcggacc ctgatagccg atgtcgttca gcgccacgat gatgtcctcg
      601 aagttgatat caccgtgccc gagcgagcgg aaatcccaga agcggcgcgg gtccgcgaac
      661 tcggtgtgtc cgccgaaaac gccgaccgtg ccgtcgccgt ggccccacca cgcgtccttc
      721 atgtggacgt ggaagatccg gtccgggaag gtgcggatga acttcacgta atccacaccc
      781 tgatagccga ggtgggaggg gtcgaagttg aacccgaagg ccggatggcc gtccaaagcc
      841 tccagcgcgc gctgcgccga cgcgatgtca aaggcgattt cggtcgggtg cacctcgagg
      901 gcgaaccgga tgccgttgtc cgcgaagaca tcgagaatcg gtttccaggt tttggcgaac
      961 tccctataac cgctctcgat ctggcccggc agacagggcg ggaacgagta gagcaggtgc
     1021 cagatcgggg agccggtgaa gccgttcacc acggtcacgc ccatattacg ggcggccttg
     1081 gccgcttttt tctgcatgtc gattgcccat ttgcgggcgg ctttggggtc gttacgcacc
     1141 ttggcgggcg cgaatgcgta atggcgggcg tcgatcgggt cgcagaccaa ctgtccggaa
     1201 agatggttcg agatggccca gcatcccaac ccgtatttct ccatcagacc ccgtttctca
     1261 tcgcagtagg ttttacttcg ggaggcctgg tcgaggtcga tgtggtcgcc ccaggtgcac
     1321 acttcgagtc cttcatatcc gaatccggac atcagctcgc aaactttttc aaacggcata
     1381 tccgcccatt gcccggtgaa gatcgttaca ggtcttgcca taatcggaat actcctttcg
     1441 cgttgccgcg gagcggacgc gccgattggc acagcacccg gcaacagagg ttatgcgggt
     1501 aaccataccg caggcggcgg caacgatcaa gagcaatccg gcggatatat gcaaaaagtt
     1561 agaattgggg cttgacccgt tccagcggat cgtggaaact atcgacactg cttcattgta
     1621 gcaccattta aacgtttgtc ccacgggaaa gaacgcgaga tgctcaacaa gttgttgtct
     1681 tttttttcaa gtgatatcgg gattgatcta ggtactgcca acacgctggt ttatgtcaaa
     1741 gaccgcgggg ttgtgatccg ggagccctct gtggtggcga tccaggaggg gaccaagcgg
     1801 attctggcgg taggcgagga ggccaagcgc atgctgggcc gcacgccggc taacatcgtt
     1861 gcgatccgtc cgatgcaatc gggggtgatc gccgacttcg acatcaccga ggcgatgatc
     1921 cggtatttca tccgcaaggt gcatgtggcc aaggtggtga agccgagggt gattgtggcc
     1981 gtgccgagca acatcacaga ggtggagaag cgcgccgtgc aggaatcggc gcggcatgcg
     2041 ggcgcacgcg aggtttttct gattgaggag ccgatggccg cggccatcgg tgtcgggctg
     2101 ccggtttcgg aaccggcagg caacatgatc gtggatatcg gcggcggaac atgcgaggtc
     2161 gcgctgatct cgctggccgg catcgtttat gcgcggagtg tgcgagttgg cggtgacgcc
     2221 atggacgaat gtatcgtcca gtacatgcgg agagtttaca atctgatgat tggagagcgg
     2281 accgcggagg agatcaaaat cacgattggt tcggcttatc ctttaggtga ggagagcaca
     2341 ctggaggtca agggccgcga tctggtggcc gggcttccca agactttaac ggtcacgtcg
     2401 gaagagattc gagacgccct gcaggagccg gtgtcctcga ttgtcgacac catccggatc
     2461 acgctggaga agtgtccccc tgagttgtcg gcggatctcg tggaccgtgg tctggtgctg
     2521 gccggtggtg gcgcgatgct acgggggctt gacaagctga tcgcgtcgca gacaggattg
     2581 cccgtcacgc tggccgatga cccgctgagc gcggtcgcgg aaggcacggg agtggtgtta
     2641 aacgagctga atttcctggc gaaaaccaag ttgggacgtt gatctgactt cggctgcttt
     2701 ctggtctctg cctgcccggc aaaacggggg gtgaccggaa atgaagcaaa cgaaattgtg
     2761 ggtcggactg ttggcggtga cgctcgcggc gctggttttt tggcgcacgg gcgcgcatga
     2821 ttttgtccgc gaagccctct atccctatca aaaagcctgc gtctggttcg agcgtgccgt
     2881 atggttgcgc gcgcgcgcca tgttttcgcg ctcccggctg gcggcccgga acgccatgct
     2941 ggagcgcgac gtggcgcgtc tgcggatggt ggcggacgat gtcgaggcgc tggaggcgga
     3001 gacccgcagg ttgcgcgcgc tgctcgactt cgtgccgccg gtgccttgtc gttggctggc
     3061 cgcccccatc ctttcgcgag gcggcacctc ggcggtctgg caacaggtgc ggcttggcaa
     3121 aggatcgctc cacggggtac gcaaaggtgc gccggttgtg gtgccggacg gtgtggtggg
     3181 gcgggtgatg gatgtctcgc tgcataccag cgacgtgatg ctgatcacgg accccaacag
     3241 ccgtgtggcg tgtgagctcg aaacgcccgg cgagggggtg ggggtggtgc gcgggatcct
     3301 gtacggcgga gggtctgtgc ctgcggccgg tcccgaactc acattgctgt atgtggtgga
     3361 gccgttgcgg ttgcggtatc tggcgcggga ttttgagccg tccccccaca cgcgggtggt
     3421 gacctccggt ttgggccaga ccttcccgaa ggggctcacg gtcgggtttc tgctggacag
     3481 ccggatcgag ccgaacgcgc tgtcgcgtga ggccgcagtc atgccggcag tggattttgc
     3541 ggcattggac gaggtgtttg tgctttcccc gggaggtacg catgcgcctt gacacggatc
     3601 tgttccggat cttcttcgcg ctcggtgtgg ccgcgctgct gcaagagatg ttccccgtga
     3661 ttcccctcgt tcccgcgaaa cccgggtttt tgactgcggt ggcgttgtat tacgcgctca
     3721 cccggccatg gcccgtggcg ctgaccgcgc tgacgtgggc gggcgcgctg accgatctgc
     3781 aggacgggct gccgctgttc tgcacgatcg gatttttgct ggtcgcgtac ggtgcggtcc
     3841 ggctgatgca gcggctgttt ctgactgcga ccgtggcgca tggcgcggtg ctggtggcag
     3901 ggatggcagc ggcgcagacg ttgtggacgc gattgtgtgt tggtgtcggc ggttggtccc
     3961 tcctgtggca gacgttagcg gcagccgggt atgcggctca tgcgggactg gttgcggggg
     4021 ggctgggatt cggtttttgc ggtctgctgg accgcgtctc aggaagcgaa agaccggtga
     4081 aagaggacaa tggaatcggg tggacagaaa cagaccagta gctggcgtat cgcattgctg
     4141 gcattggtgt tcgcggcggg tgtcgcggtc ctgggttccg ggttgtaccg ggtgcaggtg
     4201 gtgcagaacc cgtctttttc gcgggaccaa ctccgccaga gtgtgcggcg cgtgcaggaa
     4261 ccggggttgc gcggccggat tttcgaccgg aacggcatct gcctggccga gaaccgcgcc
     4321 agttactgca tcgcctttta tgtcgaggag ttgcggaaac gcggcccgtg gatccggacc
     4381 atcgatgcgg tgaatacgga gatcgaccgg ctcgcggcag tgttgggggt cccccgcgtg
     4441 atctcggttg cggcggtttc gaatcatgtg atcaacagcc ggtatatgcc gttgctggcg
     4501 tggcgcgatg tggggcagga aattctggcc cgctgggctg agactcagga agtgttcccg
     4561 ggggtggacg tgtatgtgca gccggagcga tcctatcccc agggggcgct ggccgcgcat
     4621 gtgctgggtt acgtcaagcg cgatatgccg ttgccgttgc ccggcgagcg cgttcacttc
     4681 ttcctgccgg aaatgttcgg cgcgaacgga gtcgagcggc agtacaacgc gttgctcacc
     4741 gggcgctctg gcggcaagct gatccgcgtg gacgcccgcg ggtataagca tgcggtgtat
     4801 caaggcgagg aggcggtggc ggggcaggac ctccatctga cacttgatgt gcggatccag
     4861 cgcacactgg agcaggccct gcgcggtctg cggggggcgg gggttgtact cgatccccgc
     4921 aacggcgaag tgctggccat ggcgagcgcg cccgcatacg atgcgaacgc gtttgcgccg
     4981 tatatttcca caggactttg gaggagcctg aacacggatc cgcatttgcc tctgttcaac
     5041 cgtgcgctgc agggcggata cgcgccggga agcacattca aaccggttac cgccatcgcg
     5101 gccctgatcc acggtgcggt ggagcctgag acgctccaca cctgcacggg cgtgttcgaa
     5161 ctgggaacca tgcggctgca ttgctggaac acctacggcc acggcgcgat cgcgctgcgc
     5221 aag
//
LOCUS       DAOSRB010000189         5216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_14494_length_5216_cov_7.167797, whole genome shotgun sequence.
ACCESSION   DAOSRB010000189 DAOSRB010000000
VERSION     DAOSRB010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14494_length_5216_cov_7.167797"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLZ74_09190"
     CDS             <1..299
                     /locus_tag="PLZ74_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HOR98536.1"
                     /translation="FVFRPEGVAQLFPGPVFAAGAVCLFLGNFVFLYVNLLGCCKRRQ
                     DSLMWWALLTPFYWMMMSYSGWRAVIQFFRDPFVWEKTQHGLGSPAGPAAVRRG"
     gene            343..1842
                     /locus_tag="PLZ74_09195"
     CDS             343..1842
                     /locus_tag="PLZ74_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98537.1"
                     /translation="MTEDRKTYRWVRMLTGGVTPFVLAVALLVFVRVYVSACVPGAQE
                     AGICRELLAGTTVGRQALFGSCWVAPLPVLFYLPFAWIFPEPLAGMGAFFTAWFLVLW
                     AVREAVKATGQSGVRIVLAQTAVVGMVMLSPQAQTLGIRTALMTLFMLLGGAALADWA
                     AYRRIRDVVTAGWAGALLALCGLPAFVSAGVAVGMLPLAACGKRESRGRIQAWVLLGG
                     LPLVYTVGVWILMNRLVLGDPFFFVRSLSYLLPQPGVFVLAVLGSGLALLPALIMTWV
                     CDARTPEAPGAGPVAASAVLISFAVELLVCGWVLTRFGVGWHVGTLYVAALAVLVVAL
                     ARLRQSPVRLAVALGLFVCLSRFWLGGAPVSELSAGAENADADIPRQVEAFVESRTPY
                     GRVFVLGYAGLDLLRGYTGGRLQPNLDLHVGALRRAYPGQNLYVLVPRPTGAARSESV
                     FWKYPDLYVLGGDRLLYAATFGTWRLFEVVTAPTQEQWDEWRRAAESGN"
     gene            1880..2638
                     /locus_tag="PLZ74_09200"
     CDS             1880..2638
                     /locus_tag="PLZ74_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HOR98538.1"
                     /translation="MKRESQVLAGHVAPFALWIGVLLLLQGLEAVGMCPRGLYPWSYA
                     LKTVACAALFLWFAPWRVYPALKKNQVLPALLAGAAVAGVWIVPETPWMTRVAPAFQA
                     FYHRWLILFPGTLPDFVDPAWFPALPPGHRALSYAPEQAGWGLTVCRLVGSAGVIAVI
                     EEFFFRGFFYRWLRQNRFTEVPLTVWDAQSFWIVAVVFALEHDRWAAGLLAGVVYGWL
                     TVRTGSVWAAALAHGFTNLLLGVYVIVSGQYGFW"
     gene            2669..3370
                     /locus_tag="PLZ74_09205"
     CDS             2669..3370
                     /locus_tag="PLZ74_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98539.1"
                     /translation="MKRAEAKLNREYALRLLGVGALMIGLSLWSLYDGFVAWPRQNRA
                     LDVARSALLAQGLSAEAWLDRDAEGTRPIDAVFAAQGRRAPSKLVKKLGELRVPAAAA
                     DKERVRAMQTEQVRRLFEGPVYDTRDLRSQSIQAAVTLGLALLALLTVARKARLQFAA
                     DDSGLSGSGFGNVSIPYTELARIDWSKWDTKGIVVLTLRNGRRHQLDAWHFAGMTGLA
                     EALRQHRPDLAPDTD"
     gene            complement(3402..>5216)
                     /locus_tag="PLZ74_09210"
     CDS             complement(3402..>5216)
                     /locus_tag="PLZ74_09210"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOR98540.1"
                     /translation="PYDLPIVGYGDTVNTLRLWSARASDEFHLDDFNKGSYVEAVENK
                     VLAENLTKVLYPNDNVLAGKELRLRQQYFFVSCSLQDILQRFRLKNSDWRLLPEKAFI
                     QLNETHPALIIPELMRLLVDREGLDWDTAWSITRTCTGYTNHTVLPEALEKWSVALME
                     RLLPRHMQIIYEINGRFLRQIASLWPGDMRRLARMSLIDEQGERSVRMANLAIVGSSA
                     VNGVARLHSEILKTSLFRDFYELNPKAFSNKTNGITQRRWLLKANPQLATLITEAIGD
                     GWIVDLERLRELEGRLDDTAFLAAFRKVKQANKEMLASHIHATVGIEVSPEAIFDVQV
                     KRLHEYKRQLLLALYIIVLYNRLVEDPKYKPFPRVFLFAAKAAPGYLTAKLIIRLIHG
                     IADVVNQNPHIRERLSVVFLPDYRVSLAEKIIPAANVSEQISLAGTEASGTGNMKLML
                     NGALTLGTLDGANVEIHEAVGDENIFLFGLRAEQVNELRPVYSSQAIYHADPEIRHAI
                     DMIRHNVFSLLTPGIFDPLIRSLLDYNDQYMLLADLRDYIETQDRVEALYRKPQEWDR
                     KALINVARSGWFSSDRTIREYARDIWHVEPVIPAQPES"
BASE COUNT          787 a         1374 c         1890 g         1165 t
ORIGIN      
        1 ggtttgtgtt tcgcccggaa ggggtcgccc aactcttccc cggtccggtt ttcgcggctg
       61 gcgcggtctg tctgttcttg gggaatttcg ttttcctgta cgtgaatctg ctggggtgct
      121 gcaaacggag gcaggacagc ctgatgtggt gggccttgct cacaccgttt tattggatga
      181 tgatgagtta cagcgggtgg cgggcggtca tccagttttt ccgtgatccg tttgtgtggg
      241 agaagacgca gcatggtttg gggtctccag ccggtcccgc ggcggtgcgg cggggctgag
      301 agcagaaagt gtggtggcgc gtacgagaag gcagggtagg gtatgacaga ggatcggaaa
      361 acgtaccggt gggtccggat gctgacaggc ggggtgacgc cgttcgtgct ggctgtggca
      421 ttgttggttt tcgtgcgggt ttatgtgtcc gcgtgtgtgc cgggcgcgca ggaggcgggt
      481 atttgccgcg aattgctggc cgggaccacg gtggggcggc aggcgttgtt cggttcatgc
      541 tgggtcgcgc cgttgccggt gctcttttat ctgccgttcg cttggatctt tcccgagccg
      601 ttggccggga tgggcgcatt tttcacggca tggtttctgg tgctgtgggc cgtgcgcgag
      661 gccgtcaagg cgaccgggca gtcgggtgtg cggatcgtgc tggcgcagac cgccgtggtg
      721 gggatggtga tgctttcgcc gcaggcgcag acgttgggga tccgcacggc gctgatgacc
      781 ctgttcatgc tgttgggggg ggcggcgctg gcggattggg cggcgtaccg caggatccgt
      841 gatgtggtca ctgcgggctg ggccggagcc ctgctcgcct tgtgcggatt gcccgccttt
      901 gtctcggccg gtgtggcggt ggggatgttg ccgctggcgg cctgcgggaa gcgtgagtcg
      961 cgcggccgca tacaggcctg ggtgctgctc ggcgggttgc cgttggtgta tacggtcggg
     1021 gtctggattc tgatgaaccg gctggttttg ggtgacccgt tcttttttgt gcggtccctg
     1081 tcgtatctgc tgccgcagcc cggcgtgttt gtgttggcgg tgctgggttc ggggctggcc
     1141 ctgttgcccg ccctgatcat gacgtgggtc tgcgatgcgc gcacgcctga ggcgcccggc
     1201 gcgggtccgg tggcggcctc cgctgtgctg atctcgtttg cggtggagct gttggtgtgt
     1261 ggatgggtgc tgacgcgctt cggtgtcggg tggcacgtcg ggacgctgta tgtcgcggcg
     1321 ctggcggtgc tggttgtggc tcttgcgcga ctgcggcagt cgcctgtccg cttggccgtc
     1381 gcactcgggt tgttcgtgtg cctgagtcgg ttctggctgg gcggcgcacc tgtgtcggaa
     1441 ttgtcggcgg gtgcggaaaa tgccgatgcc gacatcccgc ggcaagtcga ggcttttgtc
     1501 gaatcccgca cgccgtacgg ccgggtgttc gtgttgggat acgccgggct ggatctgttg
     1561 cgcggttata cgggggggcg gctccagccc aatctggacc tgcatgtggg cgcgttgcgc
     1621 cgcgcgtatc ccggccagaa tctctatgtg ctggtgccgc gtccgaccgg ggcggcgcgt
     1681 tcggagagcg tgttttggaa ataccccgat ttgtacgtat tgggcggaga ccggctgctg
     1741 tatgccgcga ccttcggcac ctggcggctt ttcgaggtgg tgaccgcgcc gacgcaggag
     1801 cagtgggacg aatggcggcg ggctgctgag agcggaaact gatgctgccg gggatagcgg
     1861 gtaaaggagt gccgcgcggg tgaaacggga gagtcaggtg ttggcgggac atgtcgcacc
     1921 gttcgcgctt tggatcggcg tgctgctgtt gctgcagggg ctggaggcgg tcgggatgtg
     1981 cccgcgtggc ctatacccgt ggagttatgc actcaaaacc gttgcctgtg ccgcgctgtt
     2041 tttgtggttc gcgccttggc gcgtctatcc ggccttgaaa aaaaaccagg tgctgcccgc
     2101 actgcttgcg ggcgcggcgg tggcgggggt ctggattgtg ccggagacgc cctggatgac
     2161 gcgcgtggcg cccgcgtttc aggcgtttta tcatcggtgg ctgattctgt ttccgggtac
     2221 gttgccggac tttgttgacc cggcgtggtt tccggcgctg ccgccgggac atcgagcgct
     2281 ctcgtacgcg ccggagcagg ccggatgggg tttgaccgta tgccgactgg tgggctcggc
     2341 aggcgtcatc gcggtgatcg aggagttttt tttccggggt tttttttacc gctggctccg
     2401 ccagaaccgt ttcacggaag tgccgctcac tgtctgggac gcgcagtcgt tttggattgt
     2461 ggcggtggtg ttcgcgttgg agcatgaccg gtgggccgca gggttgctcg caggggttgt
     2521 ttacggatgg ttgacggtgc ggaccggcag tgtctgggct gcagcgctgg cgcacgggtt
     2581 caccaatctg ctgctcgggg tgtatgtgat tgtgtcggga cagtacgggt tctggtgaag
     2641 tgggtgtcgg gaaaaggacg gagggaaagt gaagagggct gaggcgaagt tgaatcggga
     2701 gtatgcgttg aggcttttgg gtgtcggcgc gctgatgatc gggttgagcc tgtggtcgct
     2761 gtacgacggc ttcgtggcct ggcccaggca gaaccgtgcg ctggatgtgg cgcggtcggc
     2821 gttgctggcc caaggtcttt cagcggaagc ctggcttgat cgggatgcgg agggaacacg
     2881 tccgatagat gccgtgtttg ccgcgcaagg gaggcgcgcg ccttccaaat tggtcaaaaa
     2941 gctgggagag ttgagagtcc cggccgctgc cgctgataag gagagggtgc gcgcgatgca
     3001 gactgagcag gtccgtcgat tgttcgaggg gccggtatac gacacgcgcg accttcgatc
     3061 gcaatccatt caggctgcgg tgaccttggg tctggcgttg ctggctttgc tcacggtggc
     3121 gcgcaaggcg cggctgcagt ttgcggcgga tgattcgggc ctgagcggga gcggattcgg
     3181 gaatgtgtca ataccgtata cggaactcgc gcggatcgac tggtcgaagt gggacacgaa
     3241 gggcatcgtg gttctgacgc tccggaacgg acggcggcat cagttggacg catggcactt
     3301 tgccgggatg acggggctgg cggaggcact ccgccagcac cgcccggatc tcgctccgga
     3361 cacggactga actcccgcgc ttcgttgcgg agaccgcgcc gtcaggattc aggctgcgcg
     3421 ggtatgaccg gttcgacgtg ccagatgtca cgtgcgtatt cgcggatcgt ccggtcggag
     3481 gaaaaccagc cggaacgggc cacgttgatc agcgcctttc ggtcccactc ctgcggtttc
     3541 cggtacagcg cttccaccct gtcctgggtc tcgatgtagt cccgcagatc cgccaacagc
     3601 atgtattggt cattgtagtc gagcaaggac cggatcagcg gatcgaaaat gccgggcgtg
     3661 agcagactga acacattatg ccggatcatg tcgatcgcgt gacgaatctc cggatccgcg
     3721 tggtagatcg cctgtgagga atagacagga cgcaactcat tcacctgctc ggcccgcagg
     3781 ccgaagagga agatgttttc gtcgcccacc gcctcatgaa tctccacgtt ggcgccgtcc
     3841 agcgtgccga gtgtcagggc accgttgagc atgagtttca tgttgccggt gccggatgcc
     3901 tcggtgccgg ccagagagat ctgttcgctg acattggcgg cggggatgat cttttcggcc
     3961 agcgacacac ggtaatcggg caggaagacc acactcaggc gctcccggat atgcgggttc
     4021 tggttcacca catccgcgat accgtggatc aggcggatga tcagtttggc cgtgagataa
     4081 ccgggcgcgg ctttggccgc gaagagaaag accctcggga acggtttgta ctttggatcc
     4141 tccaccagcc ggttgtacag cacgatgatg tacagcgcga gcagcagttg gcgtttgtac
     4201 tcgtgcaggc gcttgacctg cacgtcgaag atcgcttcag gggagacctc gatcccgacc
     4261 gttgcgtgga tatgacttgc cagcatctcc ttgttcgcct gcttgacttt tctgaaggcg
     4321 gcgaggaagg ctgtgtcgtc aagacgcccc tccaactccc ggagccgctc gagatcaacg
     4381 atccatccgt cgccaatcgc ttcggtgatc agcgtcgcga gttggggatt ggccttaagc
     4441 aaccagcgtc gttgggtgat gccgttggtc ttgttggaga aagccttggg gttgagttcg
     4501 taaaagtccc ggaagaggga ggtcttcaaa atctccgagt ggaggcgcgc cacgccgttc
     4561 actgcggagg agccgacgat cgccagattg gccatccgaa cggaacgttc cccctgctcg
     4621 tcgatcaagc tcatacgggc cagacgacgc atgtctccgg gccacaggga cgcgatctgc
     4681 cggaggaaac gcccgttgat ctcgtagatg atctgcatgt gcctcggcaa cagacgctcc
     4741 atcagcgcga cgctccactt ctccagagcc tctggtagca cggtgtggtt cgtgtaaccg
     4801 gtacaggtgc gggtgatcga ccaggcggtg tcccagtcga gcccttcgcg gtccaccagc
     4861 agacgcatca gttccgggat gatcagcgcg ggatgggtct cgttgagttg gatgaaggct
     4921 ttttcgggca gcagccgcca gtcgctgttt ttgagacgga aacgctgcag gatatcctgc
     4981 agggaacagg aaacgaagaa atactgctgc cgcaaacgca gctctttgcc cgccagcaca
     5041 ttgtcattgg ggtagaggac cttggtgagg ttttcggcca gcactttgtt ttcgaccgcc
     5101 tcgacgtacg accctttgtt gaagtcatcg aggtgaaatt cgtcactggc ccgcgccgac
     5161 cagagcctca gcgtgttcac ggtgtcgccg tatccgacga tcggaagatc gtacgg
//
LOCUS       DAOSRB010000190         5163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_14669_length_5163_cov_6.012334, whole genome shotgun sequence.
ACCESSION   DAOSRB010000190 DAOSRB010000000
VERSION     DAOSRB010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14669_length_5163_cov_6.012334"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLZ74_09215"
     CDS             complement(<1..181)
                     /locus_tag="PLZ74_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98541.1"
                     /translation="MRVPPRSDPVRWRLKRRAISRITREYEQKRAALGTPVPPTLFRN
                     PKFYFAAVAGFALLGV"
     gene            complement(488..901)
                     /locus_tag="PLZ74_09220"
     CDS             complement(488..901)
                     /locus_tag="PLZ74_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOR98542.1"
                     /translation="MSQELEEGATLTLDFAKIAKIAETCKDAIPVAVQHADTREVILV
                     AYTNELAMRKAFAERTLVLWSTSRNTLWEKGRTSGETFDLLEARVNCEQNSLLYIVRP
                     RRGAICHTRNRNGEPRNCYYRRIDLDTLALTNLDP"
     gene            complement(923..2587)
                     /locus_tag="PLZ74_09225"
     CDS             complement(923..2587)
                     /locus_tag="PLZ74_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOR98543.1"
                     /translation="MHTITVTVKGQEPMVVAVGTRISELLPSVDRDGFPVLGAVVNNM
                     VQPLFMSFITDAKVEPLTIRDPRGWAIYRASLCFLLAKTVHELFPDVGCRVRNSIGTG
                     LYCTVEWEECSEDALRDQVARLKQAMLETVRQDLTISTETVSYETAVKRFRETGQTDK
                     LNLLAHRNPPVICLTRCGTFFELSQEPLVSRTGVLNLFDLTPLSCGFVLTVPTPDSPS
                     ALPPLPPFEHLFRVYREHIEWGRIVGITTVGELNQAVIEKRVDDFIQTVEALHDKKLG
                     RIADEITQREPSVRLVLVAGPSSAGKTTFSKRLITHLRVNGYRPLLISTDNYFVGDAR
                     NPRDAEGRLDYEHIEAMDLQRLNRDLLRLLAREEVHLRGFDFKTKEGYDRPETTRLPD
                     NGIIVMEGIHSLNPQLIADVPRDRTFRIYINALTQLGVDSSNRISTTDTRIIRRMVRD
                     HRFRNRPAIETLRMWPSIVRGEQRWIYPFEHLADAVFNSALDYELAVLKPLIAPLLNQ
                     IKPWDEVYIEARRLSGFLHNFTPLSPDVVPGDSILREYIGGSQLTY"
     gene            complement(2569..3906)
                     /locus_tag="PLZ74_09230"
     CDS             complement(2569..3906)
                     /locus_tag="PLZ74_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOR98544.1"
                     /translation="MNLWKDTYPLIISCARGLAPQTVGELKRLGYRIVDTTAQTVVVR
                     GTMRDMMTLNLRLRTAHRVLAPLLRTECRNLREFYDDVKSIDWENLLEPDGYFTVNSV
                     TRTDAIRDTRMAALKAKDAIADRMRQQCGSRPDSGRETHGAAIFVYWQRTELIIYLDT
                     TGGEPLSKRGYRITPGTAPLQETLAAGCVLASGWDANSPFVVPMCGSGTPAIEAALLA
                     LNRAPGSFKSHFGFMAIKGYRITIPGERAGASVRQRFGASPEQIWKDMVATARDQERR
                     EGLPPILATDIDPETLESARLNAIAAGVSEHITFAACDFEATQLPPPPGVIFLNPPYG
                     QRLASGVAVESTAETDAESAADEPASGEALEPLYRRIGDFFKRQAVGYTGCVLTGNLE
                     LSRRIGLRSSRRIPFFNGPIECRMLVFDLFEGTLKEAKKGNNPEQEASCTPLP"
     gene            complement(4010..>5163)
                     /locus_tag="PLZ74_09235"
     CDS             complement(4010..>5163)
                     /locus_tag="PLZ74_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98545.1"
                     /translation="AELEKAGVKPSQVKCYSDFRKMLDECKNLDAVTVSTPDHMHAPA
                     AIRAMKQGCHVFVQKPLVRTIWEARYFEKVAKSCGVVTQMGNQGSANNGLRRNAELLQ
                     AGVLGDVTEVHVWTNRPIWPQGIGRPEGSDPIPETLDWDSYLGTAPVRPFKAGVYHTF
                     KWRGFFDFGTGAFGDMACHTMNLPFRGLRLGAVESAECIQIEGKNNETYPNKSIVRLT
                     YAARGRLPRVTLYWYDGNLKPSAEIMPKVVATLGQVPNTGCLIMGSKGMVCSTNDYGA
                     DGYIAFNDEAKMKSMSKHEALTEAVIPAKFRPNTNGHYLEFVNACKGQDVCFSDVDHS
                     VPMLESMLVGCVAQQVPGKLKWDSRKQEFANNAAANALVKPHIRPGWEF"
BASE COUNT          957 a         1627 c         1603 g          976 t
ORIGIN      
        1 agacgcccag aagcgcgaac cccgccaccg ccgcgaaata aaacttggga ttgcggaaaa
       61 gggtcggcgg caccggagtg ccgagtgcgg cccgcttctg ctcgtactcg cgcgtgatgc
      121 ggctgatcgc gcggcgtttg aggcgccagc gcacgggatc ggagcggggc ggcacgcgca
      181 tgactttgtc gcacgccgga caacgcgacg cggggcgctc atcaaagtta tgaccgcaat
      241 atgggcaacg gtagcgcata ggaacgaaag taagaggtga taagaggcag caggcagcag
      301 gcagtgggca gcaggcagtg ggcaacaggc agtgggcaac aggcagtggg cagcaggcag
      361 tgggcagtgg gcagggggca gggggcagtg ggcaacaggt gataagcggg agcagaaact
      421 cccaactccc aactcctaac tcctaactcc taaatcctaa atcctaactc ctaaatcttc
      481 cttcccttta tgggtccaga tttgtgagtg caagcgtgtc gagatcgata cggcggtagt
      541 agcagttgcg cggctcgccg ttccggttgc gggtgtggca gatcgcgccg cgccgcggcc
      601 gcacaatgta caggagcgag ttctgctcgc agttgacgcg cgcctcgagc agatcaaacg
      661 tctcgcccga agtcctgcct ttttcccaca gcgtgttgcg cgaggtgctc cacaacacga
      721 gtgtgcgttc ggcaaaggcc ttacgcatcg ccagttcgtt ggtgtaggcc accaggatca
      781 cctcccgggt gtccgcgtgc tgcaccgcca ccggaatcgc gtctttgcag gtttcggcga
      841 ttttcgcgat tttcgcgaaa tccagcgtca gggttgcgcc ttcttccagt tcttggctca
      901 tggtgttcct ttcattcgcg cgtcagtagg tcaattggct gccgccgatg tactcacgca
      961 aaatcgaatc ccccggcacg acatccggcg acagcggcgt gaagttgtgg aggaatccgg
     1021 agaggcggcg cgcctcgata tacacctcgt cccaaggttt gatctggttg agcagcggcg
     1081 cgatcagggg cttcagcaca gccagctcat agtccagcgc cgagttgaac accgcatcag
     1141 cgagatgctc aaacgggtag atccagcgct gttcgccgcg cacgatcgag ggccacatgc
     1201 gcaacgtctc gatggccgga cggttgcgga accggtggtc ccgcaccatg cgccggatga
     1261 tccgcgtgtc ggtggtggaa atgcggttgc tgctgtccac gccgagttgc gtgagcgcgt
     1321 tgatgtagat ccggaatgtc cgatcgcggg gcacgtccgc aatcaactgg ggattgaggc
     1381 tgtgaatccc ctccatcacg atgatgccgt tgtccggcag gcgtgtggtc tccgggcggt
     1441 cgtacccctc cttggtcttg aaatcgaagc cgcgcaagtg cacctcctcc cgcgccagca
     1501 ggcgcaacag atcgcggttc aggcgctgca ggtccatggc ctcgatatgc tcgtagtcca
     1561 gccgtccctc cgcatcacgg gggttgcgcg catctcccac gaaatagttg tccgtggaga
     1621 tcagcagcgg ccggtagccg ttcacgcgca ggtgcgtgat gagccgcttg gaaaaggtgg
     1681 tcttcccggc ggaggagggt cccgccacca gcaccagccg caccgaaggc tcgcgctgcg
     1741 taatctcatc cgcaatgcgg ccgagttttt tatcgtgcaa cgcctccacg gtctggatga
     1801 agtcatcgac ccgtttctcg atcaccgcct ggttgagttc gccgaccgtg gtgatgccca
     1861 cgatccggcc ccactcgatg tgctcccgat acacccggaa gaggtgctcg aacggcggca
     1921 gcggcggcag cgcgctcggt gaatccggcg tcggcaccgt gagcacgaac ccgcaggaaa
     1981 ggggagtgag gtcgaacagg ttcagcacgc cggtgcggga gacgagcggc tcctggctca
     2041 actcgaaaaa ggtgccgcag cgcgttaggc aaatgaccgg cggattccgg tgcgccagca
     2101 ggttgagttt gtcggtctgt ccggtttcgc gaaaccgttt caccgccgtc tcgtacgaca
     2161 ccgtttccgt gctgatcgtg agatcctgcc gcaccgtttc gagcatggcc tgtttcagac
     2221 gtgccacctg atcacgcaac gcgtcttccg aacattcctc ccactcgacc gtgcagtaga
     2281 gacccgtgcc gatcgagttg cgcacgcggc agcccacgtc cggaaaaagc tcgtggacgg
     2341 ttttggccaa caggaaacag agtgacgcgc ggtagatggc ccaaccgcgg ggatcccgga
     2401 tcgtcagggg ctcgaccttc gcatccgtga tgaacgacat aaaaagaggc tgcaccatgt
     2461 tgttcactac cgcccccagc acggggaacc cgtcccggtc gaccgaaggc agcaattcgc
     2521 tgatacgagt gccgacggcc acgaccatcg gctcctgccc tttgactgtc acggtaatgg
     2581 tgtgcatgac gcttcctgtt ccgggttgtt accctttttc gcctccttca gcgtaccctc
     2641 aaacaggtca aagaccagca tgcggcactc gatcggcccg ttgaaaaaag ggatgcgccg
     2701 cgacgaacgc agcccgatac ggcgcgacaa ctcgaggtta cccgtcagga cgcaaccggt
     2761 atagcccacg gcctgacgct tgaaaaaatc gccgatgcgg cggtaaagcg gctccagcgc
     2821 ctcacctgac gcaggctcat ccgcagcaga ctcggcatcc gtttccgccg tgctctccac
     2881 tgcgacgccc gaggccagac gctgcccata gggcgggttc aggaaaatca cccccggcgg
     2941 cgggggcaac tgcgtcgcct cgaaatcgca ggcggcaaag gtgatgtgtt cggagacgcc
     3001 cgccgcgatg gcgttgagcc gcgcggattc aagcgtctcg gggtcgatgt ccgtggcgag
     3061 gatcggcggc aggccctccc ggcgttcctg gtcgcgcgcg gtagccacca tgtctttcca
     3121 aatctgttcc ggcgaggcac cgaaacgttg ccgcacactc gctcccgcgc gctcgccggg
     3181 aatcgtgatg cggtaccctt tgatggccat gaacccgaag tggctcttga agctgcccgg
     3241 cgcacggttg agcgccagca gcgcggcctc gatcgccggg gtcccgctac cgcacatcgg
     3301 cacgacaaac gggctgttgg catcccatcc cgaagccagc acgcatccgg ccgccagggt
     3361 ctcctgcaac ggcgcggttc ccggcgtgat gcgatacccg cgtttggaga gcggctcgcc
     3421 tcccgtggtg tccagataga tgatgagctc ggttctctgc caatagacaa agattgcggc
     3481 gccgtgggtt tcgcggcccg aatcgggacg actgccgcac tgctgccgca tacggtcggc
     3541 gatcgcgtct ttggccttga gcgcggccat gcgggtgtcg cggatcgcgt ccgtgcgcgt
     3601 cacgctgttc accgtgaaat acccgtccgg ctccagcagg ttttcccaat cgatggattt
     3661 gacgtcgtcg taaaactcgc ggaggttgcg gcactcggtc cgcaacagcg gcgcgagcac
     3721 acggtgcgcg gtgcggagcc gcaagttcag cgtcatcatg tcgcgcatgg tgccgcggac
     3781 caccaccgtc tgtgcggtgg tgtccacaat gcggtaaccg agccgcttga gttcgccgac
     3841 cgtctgcggc gccaacccgc gcgcgcagga gatgatcagc ggatacgtgt ctttccaaag
     3901 attcatgttc aggccttaca aaaccacaaa ggtcacaaag attccggatt ccgacctctt
     3961 cgtggccttc gtggctttgc gcaaacattc cgaccgggcg gatgccccgt cagaattccc
     4021 agccgggacg gatgtgcggt ttgaccaacg cgttggctgc ggcgttgttg gcaaactcct
     4081 gtttacggct gtcccacttc agcttgccgg ggacctgctg cgcgacgcag ccgaccagca
     4141 tgctttccag catcggcacc gaatggtcca catccgagaa gcacacgtcc tgacccttgc
     4201 aggcgttgac gaactcgagg tagtggccat tggtgttggg gcggaacttc gccgggatga
     4261 ctgcctcggt cagcgcctca tgcttggaca tcgacttcat cttcgcctcg tcgttgaagg
     4321 cgatgtagcc gtccgcgccg tagtcgttgg tgctgcagac catgcctttg ctgcccatga
     4381 tcaggcagcc ggtgttcggc acctgaccca gtgtcgcgac gactttgggc atgatctctg
     4441 ccgaaggctt gagattgccg tcataccagt agagcgtcac ccgcggcaga cggccgcgcg
     4501 cggcataggt cagccgcacg atgctcttgt tgggataggt ctcgttattc ttaccctcga
     4561 tctggatgca ctcggccgac tcgaccgcgc ccaggcgcaa accgcggaac ggcaggttca
     4621 tggtgtggca ggccatgtcc ccgaacgcgc cggtgccgaa atcaaaaaag ccgcgccact
     4681 tgaaggtgtg gtagacgccg gccttgaacg ggcgcaccgg tgccgtgccg aggtagctgt
     4741 cccaatcgag cgtctccgga atcgggtccg acccctcggg cctgccgatg ccctgcggcc
     4801 atatcgggcg attggtccac acatgcacct cggtcacatc accgaggaca ccggcttgca
     4861 acagttcagc gttacggcgc aggccgttgt tggcactgcc ctgattgccc atctgcgtca
     4921 ccacaccgca gctcttggcg accttttcaa aataacgcgc ctcccagatc gtgcgcacca
     4981 gcggcttctg cacaaacaca tggcacccct gcttcatggc gcggatcgcg gcgggcgcat
     5041 gcatgtggtc cggcgtcgaa accgtcacgg cgtccagatt cttgcactcg tccagcatct
     5101 tacggaaatc gctgtagcac ttgacctgcg agggtttgac ccccgctttc tccaactcgg
     5161 cca
//
LOCUS       DAOSRB010000191         5118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_14851_length_5118_cov_8.130160, whole genome shotgun sequence.
ACCESSION   DAOSRB010000191 DAOSRB010000000
VERSION     DAOSRB010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14851_length_5118_cov_8.130160"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1012)
                     /locus_tag="PLZ74_09240"
     CDS             complement(98..1012)
                     /locus_tag="PLZ74_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98546.1"
                     /translation="MNRKEFMKVSAFAGAAAAIGGCRRFGGFGCGGRVPYGDTIGDRL
                     WMWGHHRDSFKALKGRKEQYNLPYDTRIDMADACKFMGIPGCFVVRWTNNPKKAELPE
                     YMKQFRDMKRIGFSITDSAEESFDEKVRLGFEYADKMPNLTTLIMDDFFSYKGFGVSL
                     DAIREVKRGISERGMKLGVVLYSDANDIKPEFKSVLDLCDEVTFWFWYGKNVGTIEAR
                     VEKLKALVGSEKPILLGQYMYDFGDRKQMPGAVMENQLAQASRLLSAGAIDGLIFHCT
                     PLVDMNLDAVNVSRAWIRENAAKKWGVV"
     gene            complement(1418..2443)
                     /locus_tag="PLZ74_09245"
     CDS             complement(1418..2443)
                     /locus_tag="PLZ74_09245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR98547.1"
                     /translation="MRILMVDNMQIRQYGNLKMGPGRKLVCAAIRDNHRLAEFSDRDV
                     ARFLAPLGIRSIGARLANRKLIQTALNFKPDILFIGHCDYIRNRSLEAIRAALPSIRM
                     AHFNIDPIWMDWHIQQIRERMPVTDAIFVTTGGEALKAFCTGKNVVAYMPNPSDPAME
                     ICDNSQKQTFRHDLLFCGKEIAGDDRNAFLRLLERDLSGKLRFGIYGMFGYPPVWGAR
                     YEETLAKSKMALNLNRREEWPLYSSDRIAHLMGNGILTFLSDRGGMQRFFSEREAVFF
                     HEHGDLCEKILYYQEHDDERQAVAAAGRARYHQLFDSRRTLKFMIETLLNEPYSEDYE
                     WADHVFR"
     gene            complement(2470..3051)
                     /locus_tag="PLZ74_09250"
     CDS             complement(2470..3051)
                     /locus_tag="PLZ74_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOR98548.1"
                     /translation="MAKACDLRKGSLVGINGLPHVVEDLKVSTPSARGAASIYRFRFR
                     NLVTKTKQDVSCKGEEPFDEIDFERRAVQYLYREGDLYTFMDAEDFSQFSLSQDLIEE
                     QIPFLIEEMEGLFALVSEGKVLTVELPQKVALRIAACDPVMKGASVTSRSKPATCDTG
                     LVVQVPEYLEAGETILVDTATGAYLSRATADRM"
     gene            3187..3720
                     /locus_tag="PLZ74_09255"
     CDS             3187..3720
                     /locus_tag="PLZ74_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOR98549.1"
                     /translation="MRKTGANRVIVITGEGKGKTTAALGLVLRAVGHGLGVCVLHFIK
                     NRRDTGEARALAGLPGVEAHQCGRGFVRERSGPVFEAHARAAHAGLRLAAAKLRDPAF
                     GMVVLDEICGAVALGLLPVEAVCAAVAGAAPGTIIVLTGRDAAPELVELADTVSGITC
                     VRHGLEAGWPAQAGVEF"
     gene            4040..4115
                     /locus_tag="PLZ74_09260"
     tRNA            4040..4115
                     /locus_tag="PLZ74_09260"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4073..4075,aa:Thr,seq:ggt)
     gene            4163..>5118
                     /gene="tuf"
                     /locus_tag="PLZ74_09265"
     CDS             4163..>5118
                     /gene="tuf"
                     /locus_tag="PLZ74_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOR98550.1"
                     /translation="MAKETFVREKPHVNVGTIGHVDHGKTTLTAAITNVQALKGYNAP
                     ISYDQVAKASESAGRRDPTKILTIATSHVEYGTENRHYAHVDCPGHADYIKNMITGAA
                     QMDGAIVVVSAADGPMPQTREHILLARQVGVPAIVVFLNKVDLVDDPELLDLVEMEIR
                     ELLSKYEFPGDDIPVVRGSALPATKATSVDDPACECITKLMDALDSYIPEPERVLDKP
                     FLMPIEDVFSIEGRGTVVTGRVERGVVKVGDEIEIIGLRPTAKTTCTGVEMFRKLLDQ
                     GQAGDNIGCLLRGTKKEEVERGQVLAKPGSITPHTEFNGEVYV"
BASE COUNT          973 a         1482 c         1645 g         1018 t
ORIGIN      
        1 ttgtgcgcga gacgcggtcc gtacggacag gcgaccggat tcttttccgc atggcgcccg
       61 gtgggggatt cgccgtccgg ttcacgaaac agaggcgcta aaccacgccc cacttcttcg
      121 ccgcgttctc gcggatccag gcgcgggaga cgttcaccgc atcgagattc atatccacga
      181 gcggggtaca gtggaagatc agcccgtcga ttgctccggc agagagaagc cgcgaggctt
      241 gcgcgagttg gttttccatc accgcgcccg gcatctgctt gcggtctccg aagtcgtaca
      301 tgtactgccc gagcagaatc ggcttctccg atccaaccag tgctttgagc ttctcgaccc
      361 gcgcttcaat ggttccgacg tttttgccgt accagaacca gaacgtgact tcgtcgcaca
      421 ggtcaaggac actcttgaac tcgggcttga tatcgtttgc atcggaatag agcacgacgc
      481 cgagcttcat gccgcgctcg ctgatgccgc gcttcacctc acggatcgcg tcgagcgaaa
      541 ccccgaagcc cttgtagctg aagaaatcat ccatgatgag cgtcgtcagg ttcggcatct
      601 tgtcggcata ctcgaaacca agcctgacct tttcgtcgaa gctctcttcc gcgctgtcgg
      661 tgattgagaa accgatccgc ttcatgtcgc ggaactgctt catgtactcc ggcagctccg
      721 cctttttcgg attgttcgtc cagcggacga cgaagcaccc cggaatgccc atgaatttac
      781 aggcatcggc catatcgatg cgcgtgtcat aggggaggtt gtactgttcc ttcctgccct
      841 tgagcgcctt gaaggaatcg cggtgatgtc cccacatcca caagcggtcg ccgatcgtgt
      901 ctccgtatgg aacacggccg ccgcacccga aaccgccaaa cctccggcat ccgccgatcg
      961 ccgccgccgc acccgcgaag gcggacactt tcatgaattc ttttctgttc atcttttcgt
     1021 cctcaatcat ccccgttttc tgtaataaat tttgcaacag ccttccatac cgtttctttc
     1081 cccaattgtc cagcacatcc agagggccgt gattgcgcgg agcgcatctg gaaacaggag
     1141 aaagggagat ggaaagtgca ttagttcgtt agtgcattag tgcgttggcc taaaaggagc
     1201 agttgaacgg gtggatttct agcaaagccc ttcgtcgcaa gcgcaatcga ctgagttttg
     1261 gagaccggtg cgtatgcggg actaaggcgt ccgccaaccg gtagggacgc ccgcttctcc
     1321 cgtcagacct tatgtcgacc cgcgacccgc gacgggcgac ctgcggtgtc ggaaacgcgg
     1381 gcgtccctgt cgatcgtcgc gggtcgaatg tcgcgggtca gcggaagacg tgatccgccc
     1441 actcgtagtc ttccgagtag ggttcgttca acagggtttc gatcatgaac ttcagggtgc
     1501 gccggctgtc gaagagctga tgataacgtg cccggcccgc ggcggcgacg gcctgccgtt
     1561 cgtcgtcatg ctcctggtag tagaggattt tttcgcacag gtcgccgtgc tcatggaaga
     1621 agaccgcctc gcgttcgctg aagaagcgct gcatgccgcc ccggtcgctg agaaaggtca
     1681 ggatgccgtt gcccatcagg tgcgcgatgc ggtctgagga gtagagcggc cactcctccc
     1741 ggcggttcag gttaagggcc attttacttt tggcgagggt ctcttcgtaa cgggcacccc
     1801 agaccggcgg gtaaccgaac atgccgtaga taccgaaccg cagttttccg gacaggtcac
     1861 gctccagaag acgcaggaac gcgttgcggt catccccggc gatctccttg ccgcagaaga
     1921 gcaggtcatg ccggaacgtc tgtttttgcg agttgtcgca aatctccatg gcgggatcgg
     1981 aggggttggg catgtaggcc acaacgtttt tgccggtaca gaacgcttta agcgcctcgc
     2041 cgccggtggt gacgaaaatc gcgtcggtga ccggcatccg ctcgcggatc tgctggatat
     2101 gccaatccat ccagatgggg tcgatgttga aatgcgccat gcggatggag ggcagggccg
     2161 cacggatcgc ctccagggac cggttacgga tgtagtcgca atggccgatg aagaggatgt
     2221 ccggtttgaa gttcagggcg gtctggatga gctttcggtt tgccaggcgc gcgccgatgc
     2281 tgcggatgcc aagcggcgca aggaagcggg cgacgtcacg gtcgctgaat tcggccagcc
     2341 ggtggttgtc gcggatggcg gcgcagacca gcttacgacc cggccccatt ttaaggttgc
     2401 cgtactgtcg gatctgcatg ttgtcgacca tcaggatacg catcggggct ccttggtgtg
     2461 ggggttacgt cacatccggt cggcggtggc gcgcgagaga taagcgccgg tggcggtgtc
     2521 cacgaggatc gtctcgcccg cctccagata ctcgggcacc tgcaccacca ggccggtatc
     2581 acaggtggcg ggtttgctgc gtgaggtgac ggacgcgccc ttcatcaccg ggtcacaggc
     2641 ggcgatgcgc agcgcgacct tctgcggcag ctcgacggtg agcaccttgc cttcggatac
     2701 cagggcaaag agcccttcca tctcctcgat tagaaagggg atctgttctt cgatgaggtc
     2761 ctgcgacagg gagaactggg agaagtcttc ggcgtccatg aaggtataga ggtcgccttc
     2821 acggtagagg tactgcacgg cacgacgctc gaaatcgatc tcgtcgaacg gctcctcgcc
     2881 tttgcaggag acatcctgtt ttgttttggt gacaaggttg cggaatcgga accggtaaat
     2941 cgaggcggcg ccgcgggcgc tgggagtgga gaccttcagg tcttcgacaa cgtggggcag
     3001 gccgttgatc ccgacgaggc tgccttttct caggtcacaa gctttggcca taagcctttt
     3061 ctccgaaaag gaaaaaagta tagcgtgttg ggttcggcct gtcgagcgcg ggaaatccgc
     3121 gcttttcgcg tgtaacggaa aacgctatcc tttccttttc cccaacgcgg gaacgtaagg
     3181 aggggcatgc ggaagaccgg ggcgaatcgg gtgatcgtga ttaccggcga aggcaagggc
     3241 aagaccacgg ccgcgctggg cttggtgctg cgtgcggtgg gccacgggct gggcgtgtgc
     3301 gtcctgcact tcatcaaaaa ccgccgggac accggggagg cgcgggccct ggccggattg
     3361 ccgggggtgg aggcgcacca atgcgggcgg gggttcgtcc gggaacggtc gggtcctgtc
     3421 ttcgaggcgc acgcgcgcgc cgcgcacgcg ggattacggc tcgccgccgc caagttgcgc
     3481 gatcccgcat tcgggatggt ggtgctcgat gagatctgcg gggcggtcgc gctcggcctg
     3541 ctgccggttg aggcggtctg tgcggcggtg gccggagcgg cgcccggaac gatcattgtg
     3601 ctgaccgggc gcgacgccgc gccggagttg gtggaactgg cggacacggt cagcgggatc
     3661 acctgtgtcc ggcacggtct ggaggcggga tggcccgccc aggccggcgt ggagttctga
     3721 gccgcggcac gggcgtcacg cccgtgaaaa gcatgggcaa agatgcccat gccgcgcatg
     3781 cagggccaga aacgcataaa cctcgcgcat ccctgccgct gaccgaatgc gaaaaacttt
     3841 ttcgcgcgga ttgacaggag ggcctccttt gggtaatcta aacgagtttt ctttttttcc
     3901 gggactcggt tccgggacaa gggaaaacag acattggcaa ggttaaaggg actccagcac
     3961 gatggcctta aagccccctt ggggctggcg ggcaattggg caaggggttc tttgccccct
     4021 ttggggggtt ggggacgagg ccaacatagc tcagtcggta gagcacttcc ttggtaagga
     4081 agaggtcgtc agttcgattc tgattgttgg ctccagcttt ggcgatggat ttttttgtca
     4141 actctcacag gagaaaacca aaatggcgaa agagacattt gtccgcgaaa agccgcacgt
     4201 caacgtcggc acgatcgggc acgttgacca cggcaaaaca acgctgacgg cggcgatcac
     4261 caacgttcag gccctcaagg gctacaacgc cccgatcagc tatgaccagg ttgccaaggc
     4321 ctccgagtcg gcgggccggc gcgaccccac caagattctg acgattgcca cctcgcacgt
     4381 cgagtacggc accgagaatc gtcactacgc ccacgtcgac tgccccgggc atgccgacta
     4441 tatcaaaaac atgatcaccg gcgcggcgca gatggacggc gcgatcgtgg tcgtgagcgc
     4501 ggcggacggg ccgatgccgc agacccgcga gcacatcctg ctcgcccgtc aggtcggtgt
     4561 gcccgcgatc gtggtcttcc tgaacaaggt ggacctggtg gacgatcccg agttgctgga
     4621 tctcgtcgag atggagatcc gcgagttgct ttcgaagtac gagttccccg gcgatgacat
     4681 tccggtcgtg cgcggttccg ccctgccggc gaccaaggcc acgagcgtgg atgaccccgc
     4741 ctgcgagtgc atcaccaagc tgatggatgc gctggacagc tatattccgg agccggagcg
     4801 cgtgttggac aagcccttcc tgatgccgat tgaagacgtg ttctcgatcg aaggccgcgg
     4861 caccgtggtg accgggcgtg tcgagcgcgg cgtggttaaa gtcggcgacg aaatcgagat
     4921 catcggcctc cgtccgactg ccaagaccac ctgcacgggc gtggagatgt tccgtaagct
     4981 gctcgatcaa ggccaggccg gcgacaacat cggctgcctg ctgcgcggca cgaagaaaga
     5041 ggaggtcgag cgcggccagg ttttggctaa gccgggctcc atcaccccgc acaccgagtt
     5101 caacggcgag gtgtacgt
//
LOCUS       DAOSRB010000192         5096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_14912_length_5096_cov_6.178338, whole genome shotgun sequence.
ACCESSION   DAOSRB010000192 DAOSRB010000000
VERSION     DAOSRB010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14912_length_5096_cov_6.178338"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1313)
                     /locus_tag="PLZ74_09270"
     CDS             complement(159..1313)
                     /locus_tag="PLZ74_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOR98551.1"
                     /translation="MKIVCDRNMPFAVEAFSLLGEVVLREGRMIGPEDVREAEMLVTR
                     STTRVNAALLDRSAVRFYGSGVIGTDHIDIPYLNARQIVWTGAPGCNAESVANYVTAA
                     LLWLGGRFGFTLAGKTLGVIGVGHVGRRVCAHAKAMGMRVLANDPPRQRDPSDSEARA
                     FVPLDHVLAESDILTCHVPLTREGTDATWHLLNPSTFARLKPGAILINAARGAVIDTG
                     ALLAVLGKRVAQVVIDCWEGEPAYCPELLARTALATPHIAGHSFEGKVNGTAIVYRRA
                     CDFFGIVPAYAFALPEPPVPVWRGDAAGRPDEAVLRDLVLSVYDIEADSDRLRASCVP
                     DAPTRAAAFDRQRGGYPMRREFASTRAALRNASAALCAAVRGLGFAVEKG"
     gene            complement(1317..2351)
                     /locus_tag="PLZ74_09275"
     CDS             complement(1317..2351)
                     /locus_tag="PLZ74_09275"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98552.1"
                     /translation="MIKVWGDAEAEAVVALQRGIPLCERPFAEIGRGCGLSEEAVIAL
                     ARRLLASGEARRFGAVFDARRMGYRSVLCAVSVPSEQIVDVAAKVTICPGVTHAYERG
                     WPAELASDLPGGPRGRNWPNLWFTLAAPAGTFAAELETLRAACRPFAIHGLPAIRRFK
                     IDVVFDVRTRDRDERVGPCPPIAEPTSPSVGWDERAREVVRLMQGDLPVDARLFATVA
                     ERLGLDESALLGLLCSWRDAGVLRRVGLLLRHREIGFKANGMCCWDMSEEAVMEAGRR
                     VAAFPEVTHCYERPRMEIFPFRLYAMIHTSDWEETRQLFERISREAGLTDGHLLLSLT
                     EFKKTSMKFF"
     gene            complement(2528..4792)
                     /locus_tag="PLZ74_09280"
     CDS             complement(2528..4792)
                     /locus_tag="PLZ74_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOR98553.1"
                     /translation="MKNFFLVACLAVAASGPLVECVWAQGGAAPSRNVQPADTGLEIS
                     ADKFEVDQKTGWTTATGHVKIRTESQELEADRVRLHQETGDVQARGNVVIRQSGIGTW
                     AGDYIEYNYKTGRGLSGTGDFVAGVFRVSSREVTRREDGRYDIHNALVTTCTNAPGHR
                     HWCMTGHVRYKDNDYIEIFDAVPWLFGVPFAYLPYWFRDIDTHYGFRLIPGYTSKWGA
                     YLLGGYVFNVYDPPQGKGPKLDASTHVDYRTRRGAAVGQNLRWDLGDWGRGKIETYYA
                     WDQDPPDDSEDRNWVSDVDDERYRVRLFHEADLSPRDQFILRGTVNSDSEVRSDFFDR
                     ENRGESIPMNFVSLEHREHTWAAGVTASGPLNDFYSGVSRLPEGWLNVMPQPLWDTGV
                     NYEGQTRVGYLNRDAARYERALPDYMYYPGSWADYNLMRADTAHRVTYPVKVGEVLSV
                     VPRAGYRGTFYTESESDRNVFRHTAELGVETSLRASSEWGNGLRHVVEPYLDYSFQPS
                     HFDSDNGRVYAFDRYDRSLEWLDQFGRDGMWLPYDWHGVRPGVRNLLLTRDEKNRPRT
                     LLEWDVYGAVQFDSEGSLDEEGLRMAGTRVLYAPTEKLDLKAVGEWDTEDETVAYADL
                     SAFYQLSETFRLGGGYLGRDHVITDYDVSPIAQWNCVKENLIYGGFTHVINDTWSWST
                     YVRYDLRRNELDEVGGYVQYQLDCLVFQLRSAYVHKFDRIDRISERDDDFRVALMMWL
                     RAENRTPEDGWLTW"
     gene            4884..>5096
                     /locus_tag="PLZ74_09285"
     CDS             4884..>5096
                     /locus_tag="PLZ74_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98554.1"
                     /translation="MYPRFIDRFNLKRAVEPSTLFGFGFQGFWFLVSGFQGFWFLGFI
                     QIGVGIAIAIGIDHLTTEHFLGFQGFR"
BASE COUNT         1023 a         1728 c         1423 g          922 t
ORIGIN      
        1 ttctggtttc agggtttcaa ggtttcatcc aaatcggggt cgggatcgct atcggtatcg
       61 ggatcggtat cgtcgacccg cgacccgcga cattcgcccc gcgacgatcg acagggacgc
      121 ccgcgcagcg ggaaatcctg tcaatttaag cgctgccgtc accccttttc aaccgcgaac
      181 ccgagaccgc gcacggcggc gcacaacgcc gccgaagcgt tgcgcaacgc cgcccgcgtc
      241 gaggcgaact cgcgccgcat gggataaccg ccgcgctgcc ggtcgaatgc ggcagcccgg
      301 gtcggggcat ccggcacgca ggaggcgcgc aacctgtctg aatcggcctc gatgtcgtac
      361 acgctcagaa ccaggtcgcg caacaccgcc tcgtccggac gacccgcagc atcgccccgc
      421 cagaccggca cgggcggttc aggcagcgcg aatgcatagg caggcacgat tccgaaaaag
      481 tcgcaggcgc gacggtaaac gatcgccgtg ccgttcacct tgccctcaaa ggagtgaccc
      541 gcgatatgcg gtgtcgcaag ggccgtgcgc gccaacagct ccggacaata cgcaggctcc
      601 ccttcccagc aatcgatcac cacctgcgcc acacgctttc ccaggacggc caacagggcc
      661 ccggtgtcga tcaccgcgcc gcgggccgca ttgatcagga tcgcgccggg cttcagacgc
      721 gcaaacgtgg acggattcag aagatgccac gtcgcatccg tcccttcgcg ggtcagcggc
      781 acatggcagg tgagaatatc cgattcggcc agcacatggt cgagcggcac gaatgcacgc
      841 gcttcgctgt ccgatgggtc acgctgccgg ggcggatcat tcgccagcac ccgcatgccc
      901 atcgccttcg catgggcgca gacgcggcgt ccgacatggc cgacgccgat cacccccagc
      961 gttttacccg ccagcgtaaa tccaaaccgc ccgcccagcc agagcagggc agcagtgaca
     1021 taattggcca cactttccgc gttacatccg ggcgcaccgg tccagacgat ctggcgcgcg
     1081 ttcagatacg gaatatcgat atggtcggtg ccgatcacac ccgagccata gaaccgcacc
     1141 gcgctccggt ccagcaatgc ggcgttcact cgcgtcgtcg agcgcgtcac caacatctcg
     1201 gcctcgcgca catcctccgg tccgatcatc cggccttccc gaaggaccac ctcgccaaga
     1261 aggctgaagg cctcgacggc aaaaggcatg ttcctgtcac aaacgatttt catgcgtcaa
     1321 aagaacttca tactggtttt cttgaattcc gtcagcgaga gcaacaggtg cccgtccgtc
     1381 aaccccgcct cccgggagat ccgttcgaac agttgccgcg tttcctccca atccgaggtg
     1441 tggatcatgg cgtagagccg gaacgggaag atctccatcc ggggacgctc ataacagtgt
     1501 gtcacctccg ggaaagcagc gacgcgccgc cctgcctcca tcaccgcttc ctcggacatg
     1561 tcccagcagc acatgccatt ggccttgaac ccgatctcgc ggtggcgcag caacaatccc
     1621 acgcggcgca gaaccccggc atcgcgccag gaacacaaca ggccgaggag ggcggattcg
     1681 tccaagccca aacgttcggc caccgttgcg aacaaccgcg cgtcaaccgg cagatcgccc
     1741 tgcatcagcc gcaccacttc tcgcgcccgc tcatcccatc cgacggacgg ggacgtcggt
     1801 tccgcaatgg gcgggcaggg cccgacccgt tcatcgcggt cacgcgtgcg cacgtcaaag
     1861 accacatcga ttttaaaacg ccggatagcc ggcaacccgt gaatcgcaaa cggtcggcag
     1921 gcggcgcgca acgtctccaa ttccgcagca aaggttccgg cgggcgcggc cagcgtgaac
     1981 cacaggtttg gccagttccg cccccgcggg ccaccgggca gatcggaggc aagttccgca
     2041 ggccacccgc gttcgtacgc atgcgtcact cccggacaga tggtgacctt cgcagccacg
     2101 tccacgattt gctcagaagg caccgacacc gcgcacaaca cgctgcggta ccccatccgc
     2161 cgggcgtcga acacggcgcc gaaccgccgc gcctcacccg acgccagcag acgccgggcc
     2221 aaagcgatta ccgcctcctc cgacaacccg catcctcggc cgatctcggc gaagggtcgt
     2281 tcgcacagcg gaatcccccg ctgaagcgcc accacggcct ccgcctccgc atccccccaa
     2341 accttgatca tcctcgcatc cttttccgag gggtatcata acaaaaggcg gcttccacgt
     2401 cgaaggcggg tttccccgca tcaaaccgtg agggtcccga agaccggaac ggttcgagca
     2461 aacgcctgag tgccctttac gtcgcgtaat ccgggattgc gtcgcgcatg aaaccccgca
     2521 gcgcttctca ccaggtaagc cacccgtctt ccggggtacg gttttcggcg cgcagccaca
     2581 tcataagcgc cacgcggaaa tcgtcatccc gctcgctgat ccggtcgatc cggtcgaact
     2641 tgtgcacata ggcgctccgc agttggaaca ccagacagtc gagctggtac tgcacgtacc
     2701 ctccgacctc gtcgagttcg ttgcgacgca gatcataccg gacatacgtg ctccaggacc
     2761 aggtgtcatt gatcacatgc gtaaatccac cgtagatcag attctccttc acgcaattcc
     2821 actgcgcgat cggcgagacg tcgtaatcgg tgatcacatg gtcccgtcca aggtatccgc
     2881 cacccaaccg gaatgtttcc gagagctggt agaaggcgct caggtcggca tacgcgacgg
     2941 tctcgtcctc ggtgtcccac tcgccaaccg ccttgaggtc cagtttctcg gtcggcgcgt
     3001 agagcactcg agtgcctgcc atacgcaacc cctcctcatc caacgagccc tccgaatcga
     3061 actggaccgc gccgtacaca tcccattcga gcaatgtgcg cggacggttt ttctcatcac
     3121 gggtcaggag aagattacgc acacccggcc ggacgccgtg ccagtcgtac ggcaaccaca
     3181 tgccgtcgcg gccgaattga tcgagccact cgagtgagcg gtcataccgg tcgaatgcgt
     3241 acacacgccc gttatcggag tcgaaatgcg atggctggaa actgtaatcc agatacggct
     3301 caaccacatg acgcaacccg ttaccccatt cggaagaggc gcgcagcgaa gtctcgaccc
     3361 caagttcagc cgtatgccgg aacacgttcc ggtccgactc gctttccgta taaaaggtcc
     3421 cgcgatatcc ggcccgaggc accacgctaa gcacctcccc caccttgacg gggtaggtca
     3481 cacggtgcgc agtgtccgcg cgcatcaggt tgtagtcggc ccaagacccg ggatagtaca
     3541 tgtaatccgg taatgcccgc tcataccggg ccgcatcgcg gttaaggtaa cccacccgcg
     3601 tctggccctc gtagttgacc cccgtgtccc acagcggttg cggcatcaca ttcagccagc
     3661 cttccggaag ccgcgacacg ccgctgtaaa aatcgttcag cggcccgctg gcggtcaccc
     3721 cggcggccca cgtgtgctcg cggtgttcca gcgagacgaa gttcatgggg atactttcac
     3781 cgcggttctc ccgatcgaag aaatcgcttc taacttccga atcgctgttg accgtgcccc
     3841 gcaggatgaa ctggtcgcgt ggcgaaaggt ccgcctcatg aaacagccgc acgcggtaac
     3901 gctcgtcatc cacatctgaa acccagttcc gatcctcact gtcatcgggc gggtcctgat
     3961 cccaagcgta gtaggtctcg atcttgccgc gtccccagtc ccccagatcc cagcgcagat
     4021 tttggcccac cgcggccccg cgccgggtgc ggtagtccac atgtgtggac gcgtcgagtt
     4081 tggggccttt gccctggggc ggatcgtaca cgttgaagac atatccgccc aacaggtatg
     4141 cgccccattt ggaggtgtag ccgggaatca ggcggaaccc gtaatgcgtg tcgatgtcgc
     4201 ggaaccaata cggaagatag gcgaagggca caccgaacaa ccagggcacc gcgtcaaaaa
     4261 tctcgatata gtcgttgtcc ttgtatcgca catgcccggt catgcaccag tggcggtggc
     4321 cgggcgcgtt ggtgcaggtg gtgaccagcg cgttgtggat atcataccgc ccgtcttccc
     4381 tccgcgtgac ctcccgggaa ctgacgcgga acacgccggc cacgaaatca ccggtcccgg
     4441 agagaccgcg cccggtcttg taattgtatt cgatataatc tcccgcccag gtcccgatgc
     4501 cgctctgacg gatcacgacg tttccgcgcg cctgcacatc acccgtttcc tgatgcaggc
     4561 gcacacggtc ggcttcgagt tcctgcgatt cggtccggat cttcacatga cccgtggccg
     4621 tagtccatcc ggtcttctga tcgacctcga atttatcggc cgagatttcc agtccggtgt
     4681 cggcgggctg cacgttcctg gagggggccg cccccccctg cgcccacacg cactccacaa
     4741 gcggtccgga tgccgcaaca gccagacacg ccacaaggaa gaagtttttc atggttgtac
     4801 cgcgctcatt gtgaacgttt cgctaaggag tccatcgatt acagcgtaag tgaatccacc
     4861 gtgactgaca agcggaattt caggtgtatc cccgattcat cgaccgattc aacctaaaaa
     4921 gagcagttga accttcaact ctctttggtt tcggatttca gggtttctgg tttctggttt
     4981 cagggtttca gggtttctgg tttctgggtt tcatccaaat cggggtcggg atcgctatcg
     5041 ctatcgggat cgatcattta accacggaac actttcttgg gtttcaaggt ttcagg
//
LOCUS       DAOSRB010000193         5073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_14991_length_5073_cov_7.327820, whole genome shotgun sequence.
ACCESSION   DAOSRB010000193 DAOSRB010000000
VERSION     DAOSRB010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14991_length_5073_cov_7.327820"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1516)
                     /locus_tag="PLZ74_09290"
     CDS             complement(245..1516)
                     /locus_tag="PLZ74_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98555.1"
                     /translation="MMGSRRSFLKTAAASAAAWNIIPARVLGKDAPSNRIAMAMIGVG
                     GMGQGNMSSFLGMKDVIVRMVCDVNTSKTEQAKQKVDKHYGNSDCLVTKDFREVCARG
                     DIDAVMIATPDHWHAYVGTFAARHGKDIYGEKPFTHDLREGRALVRAVEQHGRIWQTG
                     SWQRSSSEMRRAVELVRNGRIGKIARVEVGLPPGGRPRNPIDPHATIPKGLDWDFWLG
                     PAPYRPYQGIADWDWRWVLDWGGGQMMDWIGHHADIAQWGLDRDTSGPVAFEGYAPFE
                     ADGIFDSPKTYRFSCRYADGVEMTVADSRQVKSGTTWYGENGEWVWVDRGRFDASTPE
                     IRDSRIEPGELRLHSSGHHRNFIDCVKTRATTLTPAEVAHRSASIGHLGQIAMLTGRK
                     IKWDPVREQILGDVSAEALLGRTPRGPWSLV"
     gene            complement(1557..4718)
                     /locus_tag="PLZ74_09295"
     CDS             complement(1557..4718)
                     /locus_tag="PLZ74_09295"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOR98556.1"
                     /translation="MYNRNVILRTLCIIYAISGAALVASAQTLHPVPQADAERIGELV
                     ETWVPARSSRARRVLVFWRCEGFVHGRAIEYGNRALEIAASQTRAFTADFSREYEALT
                     PERLARYDALVLNNTTALNTRQHGFLEPALTAFVRSGKGLAVIHAGADNFYHAEQAAE
                     MVGGRFWGHPWGDGGMWAFKLDEPDHPLNRAFGGQGFLFGDEIYQQQSPFYNRAKLRV
                     LVSLDLSHAATAAATGQRRADNDYAVSWIRPYGRGRVFYTSFGHDARAFLDRATLIHI
                     LDGVQYAVGDFRVDDTPSGLRAADLDRVRAATDLTVAEIAHDLREILTRTGHAAVDAA
                     NRALLIGLLKENGVPPYGRQAVLRAMTAAGETVPVDVLATCLAIPETREGALAQLAAT
                     PGEASSRVLSLAVPVAGPELRCALLNALAQRREAAAIAPYVADTEERVATAALAGLGR
                     IGDKTALAVLQKPVRPALEPVRLTALAACLGTLAEQDGTRAAARAARPLFAHAGTPFP
                     VRAAAARVLLQADCGFFAEGMADGCPHVRQTVVRAADRVPLRKLVAALHTAAPTDQVA
                     LLAKLAERDARSYVGEVVAKLEGEQEIVVVAALHALGRLGGCKEVPVMVSLITREGVV
                     GKAAREALLALRAPGVGAALLDVAARDPALQTRLLTLVGERGEVALLPRIRPCLRSER
                     AEVRKEAWKTLGLLADGQMYATLFTELRHLQAEEQVQAEAALQAAGKRVDPAARSVVL
                     LDTWGEKTLGVPARNALIALMAVYRDPAFVAPLSTALKEMDAGVREGALRALADWTDM
                     RPYPVLKEAVSAQPDARLRQLALRGAVKLVSAHGGAETQGRFLELLRTAPDDKGRESV
                     AEAMFLRDGLGFFPVLQTLFGDAACGPFAKRLYLRFYDEKVKSRADQPWREVAPSLWR
                     ANASHAAADAAKAFDRKPETRWSSNRASEEGMWFTLDLGENLCVSQVVLDTTRSASDT
                     PNGFEVLISDDGKTWRGPVAKGDGTQKEKNVIPMLTQGRHLKIVTTGGRPGKHWSIHE
                     IQIMAGFDPKKVAEIRQVAESLR"
BASE COUNT          946 a         1659 c         1552 g          916 t
ORIGIN      
        1 agcctttgag attgcgggcg ccgcccgcat ctcaatacgt caaaacccaa tgtttacggg
       61 gctttgctag aattctaccc gttcacctgc tccttttagg attaacgaaa caacttcggc
      121 gttctgtata agtctgcgga tcagattgcc tgatcgtttg ttcccgaaca cgacatgagg
      181 ctctgcggac gcctcgggga tgcgtcccta ccggctaacg aacaacacac gacctgaggc
      241 aggatcacac cagagaccac ggcccgcgcg gcgtacgtcc gagcagggct tcggcggaga
      301 cgtcccccag aatctgttcg cggaccggat cccacttgat tttccggccg gtgagcatgg
      361 cgatctgtcc aagatgtccg atgctcgcgg aacgatgcgc cacctcggcg ggcgtgaggg
      421 tggtcgcgcg cgtcttcacg caatcaatga agttgcgatg atgaccggaa ctatgcaggc
      481 gcaactcccc gggctcgatg cggctgtcgc ggatttcagg cgtggacgcg tcaaaccggc
      541 cgcgatccac ccagacccat tcaccgttct ctccgtacca ggtggtgccg cttttgacct
      601 gcctgctgtc ggccaccgtc atttcgaccc cgtcggcata gcggcaggag aaacgatagg
      661 ttttgggcga atcgaaaata ccgtcggcct cgaacggcgc gtatccctcg aacgcgaccg
      721 gacccgacgt gtcgcggtcc agtccccact gcgcgatatc ggcgtggtgg ccgatccaat
      781 ccatcatctg tcccccgccc cagtccagta cccagcgcca atcccagtcg gcgatgccct
      841 gatagggccg gtacggtgcc gggccgagcc agaaatccca atccaggcct ttgggaatcg
      901 tcgcgtgcgg atcaatcggg tttctggggc gtccgcccgg aggcagcccg acctccaccc
      961 gcgcgatctt gccgatccgc ccgttgcgca ccagctccac cgcacgccgc atctcgcttg
     1021 acgaacgctg ccagcttccg gtttgccaaa tgcgtccgtg ctgctccacg gccctcacca
     1081 gtgcccgccc ttcgcgcaga tcatgcgtga agggcttttc gccgtagatg tccttgccgt
     1141 ggcgggccgc gaaggttccc acataagcat gccagtggtc gggcgtggca atcatcaccg
     1201 catcgatgtc gccgcgggca cagacctcgc ggaaatcctt ggtcaccagg cagtcactgt
     1261 tgccgtagtg cttgtcaacc ttctgtttgg cctgttccgt ttttgaggtg ttgacgtcgc
     1321 agaccatacg gacgatcaca tccttcatgc ccagaaagct gctcatattg ccctgcccca
     1381 tgccgccaac gccgatcatg gccatggcga tacggttgct gggcgcgtcc tttccaagca
     1441 cccgcgccgg gatgatattc catgcggcag cggaagcagc ggccgtcttg agaaaacttc
     1501 tgcgtgaacc catcatgagg cacctctttt cttttgcaag atacgatgtt tcaaaattaa
     1561 cgtaacgact cagcgacctg ccggatctct gcgactttct tggggtcgaa gcccgccatg
     1621 atctggatct catgaatgga ccagtgcttg ccaggacgcc cgccggtggt cacgatcttg
     1681 agatggcgcc cctgcgtcag catcggaatg acgttttttt ctttctgtgt gccgtccccc
     1741 ttggccaccg gtccccgcca cgttttccca tcgtccgaga tcaagacctc aaagccgttc
     1801 ggcgtgtcgc tggctgatct tgtggtgtcg aggacaacct gcgacacaca gagattttca
     1861 ccgaggtcca gcgtgaacca cataccctct tccgaagcgc gattggacga ccagcgcgtt
     1921 tcgggtttgc ggtcaaaggc tttcgccgca tcggcggcgg cgtgcgaggc attcgcccgc
     1981 cacagggagg gcgcgacctc gcgccaaggc tggtcggcac gggacttcac cttttcgtca
     2041 tagaaccgca gatagagacg cttggcgaaa ggaccgcagg cggcatcacc aaacagcgtc
     2101 tgcaaaaccg ggaagaaccc gagtccgtca cgcaggaaca tcgcttcggc cacactctca
     2161 cgccccttgt catcaggagc ggtccgcaac aattcgagga accgtccttg cgtctccgcg
     2221 ccgccgtgtg cggagacaag tttgaccgca ccgcgcaaag cgagttggcg gagccgcgca
     2281 tccggttgcg cgctcaccgc ctctttcaac accgggtagg gccgcatgtc cgtccaatcg
     2341 gcgagtgcgc gcaacgcgcc ttcacgcaca ccggcatcca tctccttcag ggccgttgaa
     2401 agcggcgcga caaaagcggg gtcgcgataa acagccatca gggcgataag cgcgttccgt
     2461 gccggcacac cgagcgtctt ctccccccac gtgtccaaca ggacaacgga gcgggcagcc
     2521 ggatcgaccc gttttcccgc agcctgaagc gccgcttcgg cctgcacctg ttcctcggcc
     2581 tgaaggtggc gcagttccgt gaacagcgtc gcgtacatct ggccgtcagc cagaaggccg
     2641 agcgttttcc acgcctcttt ccggacctcg gcgcgctctg aacggaggca gggcctgatc
     2701 cgcggcaaca gcgcaacctc gccgcgttcg cccacgaggg taagcagccg cgtctgcaac
     2761 gcgggatccc ttgcggccac gtccagcagt gccgcaccca ccccgggcgc gcgcagcgcc
     2821 agcaacgcct cgcgggcggc tttgcccacc acaccctcgc gtgtgatcag cgagaccatc
     2881 accggaacct ctttgcagcc gcccaacctg cccaacgcat gcaacgcggc cacaacgacg
     2941 atctcctgtt ctccctccag ttttgccacc acttcgccga cgtatgaacg ggcatcccgc
     3001 tcggccaact tggcgagaag cgccacctga tccgtaggcg cggcggtgtg gagcgcggca
     3061 acgagtttgc gaagcggtac gcggtcagcc gcgcgcacca cggtctgtcg gacgtgcgga
     3121 cagccgtccg ccatcccttc ggcaaagaaa ccgcagtcgg cctgcaacag cacacgggcc
     3181 gcggcggcgc gtaccgggaa gggcgtcccg gcatgtgcga acaacggacg cgccgcgcgt
     3241 gcggcggcac gcgtgccgtc ctgttccgca agggtgccaa gacaggccgc gagtgcggtc
     3301 aggcgcaccg gctccaacgc gggccgcacg ggcttttgca ggaccgcgag cgcggttttg
     3361 tcgccgatcc ggcccagtcc ggcgagtgcg gcggtggcca cccgctcctc ggtatccgcg
     3421 acatagggcg cgatggccgc ggcctcgcgg cgctgtgcca aggcattgag cagcgcacag
     3481 cgcagctccg gtccggcaac cgggacggcc agcgacaaca cgcgggagga ggcttccccc
     3541 ggggttgcgg caagttgcgc gagcgcccct tcgcgcgttt ccggaatcgc gaggcaggtt
     3601 gccagcacgt cgacgggcac cgtctcgcct gcggcggtca tcgcccgcag cacggcctgc
     3661 cgcccgtacg gcggcactcc gttctctttg agcagtccga tcagcaaagc gcggttggcg
     3721 gcatcgacgg cagcgtgtcc ggtacgggtg aggatctccc gcaaatcatg cgcgatctca
     3781 gccacggtaa ggtccgtggc ggcgcgcacg cgatccaaat ccgcagcgcg taacccggag
     3841 ggcgtatcgt ccacccgaaa gtcgccgacg gcatactgca caccgtcgag aatgtggatc
     3901 aacgtggcac ggtcaaggaa ggcgcgcgcg tcgtggccga acgaggtgta gaaaacgcgg
     3961 ccccggccat acggccgaat ccaggaaacc gcgtagtcgt tgtcggcgcg gcgctgtccc
     4021 gtcgccgcgg cggtggccgc atgggaaagg tccagcgaga caaggacgcg caatttggcg
     4081 cggttgtaga agggcgactg ctgctggtag atctcatccc cgaacaggaa cccctgaccg
     4141 ccgaaggccc ggttgagcgg atggtccggc tcatccaatt taaaggccca catgccgccg
     4201 tcgccccaag ggtgacccca gaaacgaccg ccgaccattt cagccgcctg ttcggcgtgg
     4261 tagaaattgt cggcaccggc atggatcacg gccaagccct tgccggagcg gacgaacgcc
     4321 gtcaacgcag gttcgagaaa accgtgctgc cgcgtgttca gagccgtggt gttgttgagc
     4381 acgagcgcat cataccgggc gagacgttcg ggcgtcaggg cctcgtactc acgcgagaaa
     4441 tcggctgtga acgcgcgggt ctgggaggcg gcgatctcta acgcgcgatt gccgtattcg
     4501 atcgcccggc cgtggacaaa gccctcgcag cgccaaaaca cgaggacgcg gcgcgcccgg
     4561 gatgaccggg ccgggaccca cgtctccacc aactcgccga ttcgttcggc gtcagcctgc
     4621 ggcacggggt ggagtgtctg ggcggatgcg acgagcgccg cgccgctgat ggcgtatatg
     4681 atgcagagcg tacgaaggat aacgttacgg ttatacatga ggagcctttc cgtaaaagga
     4741 atgaaatgat tgtggaaata taatacgtgg ttccgcatct gtccagtatc gcgagagatt
     4801 ttgatccggt atcgcgccac tcgtctaccg caatcaggtc cggaatgtct gacgctccgt
     4861 gcgtgttttg agatgcaggt agggttaacc taagaagagc agttgagggt tcacgtttca
     4921 tccgcagatt gacgccgata agcgcagatt tttggggagc gagtgtctcc atccccgtgc
     4981 acggcgcggt ttttgtcact gtgctctaag ttgcgtttgc accaaacaga tgcggcgcgg
     5041 atcgcagcca aagccaatgt ttatgagacg cgg
//
LOCUS       DAOSRB010000194         5051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15080_length_5051_cov_5.874700, whole genome shotgun sequence.
ACCESSION   DAOSRB010000194 DAOSRB010000000
VERSION     DAOSRB010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15080_length_5051_cov_5.874700"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="PLZ74_09300"
     CDS             <1..1900
                     /locus_tag="PLZ74_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98557.1"
                     /translation="GITPDPRDLAEWRRAFPDGTAPSGRFETGGGTTFYGKHRGTFYA
                     LSSGGVREVIPETDYAMGWAALLLSQRPYARTVLLLGEVSLATGLALESLRPDLEIVW
                     CPADPGYGVGLLKSVKAAGMATRIRAAGVTPRRYLADPASPLFDAVLVWPPAATSLAG
                     AVWRETAFLCAVRQVTRRTGVALFGLDCTSAVVTHEQGALLERFVRRIRQVWPESGAL
                     AAGAGGWWVAAQVPGLAYGAEDAVRRFALLKSAHYPAEAVRLLYDPARAEAWRTRWPA
                     WLATDPPLAPPEMSAPETTLAYGLADALSRTYPETAAGSVFLRLLRPEGVRLTAYLLL
                     ILWTVPVVVGRAQTAPRRMVAAWLAACGALGLTASLTVLSRLEMRFGSLYLLAGAGSC
                     LYLAGLFCGNRLAEGGMRAMPERPVVTAALPSALTVLLAGCAFAMSAGAERAATAYVT
                     LLLCFPIGVAAGGALPVAWACCGEGPENRAAVFVLADALGAAAAGMFFLVLVPLAGLE
                     RTIGTFAALALGFAVCLAFGGSRAARLSAGTALMLACAVCVAQWRELAQARPEEVPGG
                     VGGAASVAVPAARGVRMKEGEGPRSSKPAADACLPGIPRRIDSERVLRQMEAGRLATN
                     EAAYWERE"
     gene            1964..3415
                     /gene="lysS"
                     /locus_tag="PLZ74_09305"
     CDS             1964..3415
                     /gene="lysS"
                     /locus_tag="PLZ74_09305"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOR98558.1"
                     /translation="MSEEQPQSGNEYRAQRIANLRSLEERGFAPYGQAFERTRLSEIR
                     STFTEDRVVRAAGRLMTIRRMGKASFATFSDGTGNFQIFVKKDRLTPEQFDAFAYLDL
                     GDFIGVEGRLFTTRTGEPTLEVHTWVLLGKAILQPPEKFHGLQDVEERYRKRYLDLMM
                     NPESRARFDKRSAILAEIRAFLHARGFIEVETPIMQPQAGGAAAKPFVTHHNALDTEM
                     VMRIAPELYLKRLIVGGYDRIFELGKDFRNEGIDRTHNPEFTVLEVYEAYGDRSSMKS
                     LIEGLMPHLCEKVLGTLQVAYGEAKEMLDFTPPYREIAYRDAVREVMGPDWFDLPVDE
                     ARARAEAKGVDIDPSWNHLMVTHEVYGKVVEKMLRQPTFVTRIPRQFVPLAKTCADDP
                     ELADVFEFVVAGKELCPGYTEQNDPMAQRAALADQAGDNPEKIDEDFITALEHGMPPT
                     GGLGMGVDRLVMMLTGTEVIRDVILFPQMKKQG"
     gene            3427..4164
                     /locus_tag="PLZ74_09310"
     CDS             3427..4164
                     /locus_tag="PLZ74_09310"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOR98559.1"
                     /translation="MNSVVKISAAAVILALGMILSSSLLSKLFVRIRHEQTITVKGYA
                     EKDITSDVGKFTATCTVRGSSLKESYDKLRESRNAVMAYLKRSGFGEAELVAGTIQTE
                     KIPKRDAQGKETNETEYFDATQTFTVTSGNVRLIRDVSNGITELIKDGIEIRAGSPEF
                     YISDLKDTKLALLAEATSDGYRRAVALAANSKGRVGSLISAQQGVFQITERHSTDTSG
                     YGAYDTSTIEKTAKAVVTLGYAIEPEK"
     gene            4193..>5051
                     /locus_tag="PLZ74_09315"
     CDS             4193..>5051
                     /locus_tag="PLZ74_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOR98560.1"
                     /translation="MGRSQIRQTDGDMGTRAILESISDGVFTVDAAWRVTSFNRAAET
                     ITGVPRKEAIGRRCADVFRSNMCGPECALQTTLKTGRPVIGRSGYIINAEGDRIPISI
                     STAVLKDHAGRVIGGAEVFRDLSEVDALRRELEGRFQVGDLVSRSPVMQRLFEVLPAI
                     AASPSTVLVQGETGTGKELVARTLHTLSPRAQAPFVAVNCGALPDTLLESELFGYKAG
                     AFTGATRNKPGRFALAKGGTLFLDEIGEISPALQVRLLRVLQERVYEPLGATRSEKAD
                     VRVVVATNRD"
BASE COUNT          846 a         1450 c         1804 g          951 t
ORIGIN      
        1 ggggatcacg ccggacccgc gcgaccttgc ggagtggcgg cgcgccttcc ccgacggcac
       61 cgcgccgtcc ggacggtttg agacaggcgg cggtacgacg ttctacggga aacaccgcgg
      121 caccttctat gccctctcct ctggcggggt ccgggaggtg attccggaaa ccgactacgc
      181 aatgggatgg gccgccctgc tgttgtcgca gcgcccttac gcccgcacgg tgctgttgct
      241 gggcgaggtg tcgctggcaa cgggactcgc gctggagtcg ctgcgtccgg atctggaaat
      301 cgtgtggtgc ccggcggacc ctggctatgg tgtcgggttg ctgaaaagcg tgaaggcggc
      361 gggtatggca acccgtatcc gcgcggccgg tgtcacgccg cggcgttatc tggctgatcc
      421 cgcatcgcct ctttttgatg cggtgttggt gtggccgccc gccgccacgt ctctggccgg
      481 cgcggtatgg cgcgagacgg cctttctgtg tgcggtgcgg caggtgacgc ggcgtaccgg
      541 cgttgcgttg ttcgggctcg attgcacaag cgccgtcgtg acgcatgaac agggcgcgtt
      601 gctggagcgc tttgtccggc ggatccggca ggtctggccg gaaagcggcg cgcttgcggc
      661 cggtgccggg gggtggtggg tggctgcgca ggtgccggga ttggcgtatg gagcggaaga
      721 cgcggtacgt cgtttcgccc tgctcaagag tgcgcactat ccggccgagg cagtgcgcct
      781 gctctatgat cccgcgcgcg cggaagcatg gcgaacgcga tggcctgcgt ggcttgccac
      841 ggaccccccc ctggccccgc cggagatgtc cgcgccggaa acgacgttgg cctacggctt
      901 ggctgacgcc ttgagccgca cgtatcccga aactgcggcg ggatccgttt tcctgcggtt
      961 gctgcggccc gagggtgtcc gtctgacagc ctatctgctg ctgatcctct ggaccgtgcc
     1021 ggtggtcgtg gggcgcgccc agaccgcgcc gcgccggatg gtggccgcat ggctcgcggc
     1081 gtgcggcgct ttggggctga ccgcgtcgct cacggtgctg agccggctcg agatgcgttt
     1141 cggttcgctc tacctgttag cgggtgccgg gagctgcctg tatctggcag ggcttttttg
     1201 cggcaaccgt ttggccgaag gggggatgcg cgccatgccg gagcgtcccg ttgtcacggc
     1261 tgcgttaccg tcggccttga ccgtgctgct ggcgggatgt gccttcgcga tgtcggctgg
     1321 cgcggaacgc gccgccaccg cttatgtaac cctgctgctc tgttttccga tcggggtcgc
     1381 ggccggaggc gcgctacccg tggcgtgggc gtgctgcggg gaaggaccgg agaatcgcgc
     1441 cgcggtcttt gttctggccg atgcgttggg tgcggcggcg gcggggatgt tctttctggt
     1501 attggtgccg ctcgccgggt tggagcggac gattggcact tttgcggctt tggcgctcgg
     1561 cttcgcggtc tgtctggcgt tcggcggcag tcgcgcggct cggctgagtg ccggcacggc
     1621 gctgatgctg gcttgcgcgg tttgcgtggc tcaatggcgc gaacttgcgc aagcgcggcc
     1681 ggaagaggtg cccggcggtg tcgggggcgc ggcatccgtt gcggttcccg cggcacgcgg
     1741 cgttcgcatg aaggaagggg aggggccgcg ctcctccaag cctgccgcgg acgcgtgcct
     1801 gcccggcatt ccgcgccgga tcgattccga gcgcgtgttg cgccagatgg aggcggggcg
     1861 gttggccacc aacgaggcgg cgtattggga acgcgagtag tggcagggct cccgaaggtg
     1921 tgatacactg ccgcgcatgg ttgaaaggaa agggacggtc gagatgtcag aggagcagcc
     1981 gcagtcggga aacgagtatc gcgcacagcg catcgctaat ctgcgcagtc tggaggagcg
     2041 ggggtttgcg ccctacgggc aggcgtttga gcgcacgcgc ctttcggaaa tccgctccac
     2101 gtttacggag gatcgcgtgg tgcgggcggc cggtcgcctg atgaccatcc ggcgcatggg
     2161 caaggcctcg ttcgcaacgt tcagcgacgg caccggcaat tttcagattt tcgtcaagaa
     2221 ggatcggttg accccggaac agttcgatgc gttcgcctat ctcgatttgg gggatttcat
     2281 tggtgtcgag ggtcgcctct tcacgacgcg taccggcgag ccgacccttg aggtccacac
     2341 gtgggttctg ctgggcaagg cgatcctgca accgcccgag aagttccacg ggttgcagga
     2401 tgtcgaagag cgttaccgca aacgttatct ggatctgatg atgaatcccg agtcgcgtgc
     2461 ccggttcgat aagcggagcg cgattctggc ggagatccgc gctttcctgc acgcgcgcgg
     2521 gttcatcgag gtcgagacgc cgatcatgca gccgcaggcg gggggtgcgg ctgccaaacc
     2581 gttcgtcacg catcacaacg cgctggacac cgagatggtg atgcgtatcg cgcctgagtt
     2641 gtatttgaag cggttgattg tcggcggata cgaccgcatc ttcgagttgg gcaaagattt
     2701 ccggaacgag gggatcgacc gcacacacaa tccggagttc accgtgctgg aggtctacga
     2761 ggcctacggc gaccgttcct ccatgaagag cctcattgaa ggactcatgc cgcacctgtg
     2821 cgaaaaggtg ctcggcacgc tgcaggtggc gtacggtgaa gccaaggaga tgctggactt
     2881 caccccgccc taccgcgaga tcgcgtatcg ggacgcggtg agggaggtga tggggccgga
     2941 ctggtttgat ctgccggtgg acgaggcgcg ggcgcgtgct gaggcgaagg gggtggacat
     3001 cgacccgtcg tggaaccatc tgatggtcac gcatgaggtc tacggcaaag tggtcgagaa
     3061 aatgctgcgt cagcccacgt tcgtgacgcg catcccgcgc cagttcgtgc ctctggccaa
     3121 aacctgcgcg gacgatcccg agttggcgga cgtcttcgag ttcgtggtgg ctggcaaaga
     3181 gctttgtccg ggttataccg aacagaacga tcccatggcg cagcgcgcgg cgctggctga
     3241 tcaggcgggg gataaccccg aaaaaatcga tgaggatttc atcacggcgt tggagcacgg
     3301 catgcctccg accggagggt tgggcatggg ggtcgaccgg ctggttatga tgctcaccgg
     3361 aaccgaagtg atccgggatg tgatcctctt cccccagatg aaaaaacagg gttgacggag
     3421 agacgaatga atagcgttgt caaaatatct gcggctgcgg tgatcctggc tttggggatg
     3481 attctttcct cttccctgtt gtccaaactg ttcgtccgga ttcggcacga acagaccatt
     3541 acggttaagg ggtacgcgga aaaggatatt acgtccgacg tcgggaagtt caccgccaca
     3601 tgcacggtca ggggttccag tctgaaggag tcctacgaca aactgcggga aagcaggaat
     3661 gcggtcatgg cgtacctcaa gcggtcaggc tttggcgagg cggagctggt tgcgggaacc
     3721 attcagaccg agaagattcc caaacgggat gcgcagggga aggaaaccaa tgaaaccgag
     3781 tatttcgacg ccacgcagac cttcaccgtc acgtccggca atgtccggct gatccgcgac
     3841 gtttcgaacg gaatcaccga attgatcaag gacgggatcg aaatccgggc cgggagcccc
     3901 gagttctaca tctccgacct gaaggatacg aaactggcgc ttctcgccga agcgacatca
     3961 gacgggtatc ggcgtgcggt ggcccttgcc gccaacagca agggcagggt cgggagcttg
     4021 atctccgcgc aacagggggt ttttcagata accgaacgcc actccacgga cacgtccgga
     4081 tacggcgcgt acgacacgtc gacgatcgag aaaacggcca aagcggtcgt gacgctcgga
     4141 tacgcaatcg aaccggaaaa ataggccgga atcagacggg aagggtgtgg gcatgggtcg
     4201 gtcccagata cggcagacag acggcgacat gggcacacgc gccattctgg aaagcatctc
     4261 ggatggcgtc ttcacggtcg atgcggcgtg gcgcgtcacc tcgtttaacc gggcggccga
     4321 gaccattaca ggggtgccgc ggaaagaggc gatcggccgt cgatgcgcgg acgtcttccg
     4381 ttccaacatg tgcgggcccg aatgcgcgtt acaaacgacc ttgaagaccg gtcggccggt
     4441 gatcggccgc tcgggataca tcatcaacgc cgaaggggat cgcattccga tcagcatctc
     4501 cactgcggtg ctgaaggatc atgcgggtcg ggtcattggc ggcgcggagg tgttccgcga
     4561 tctttccgag gtggatgccc tgcggcgcga gctggagggc cggtttcagg tcggcgatct
     4621 ggtcagccgc agtccggtga tgcagcgcct gtttgaggtg ttgcccgcga tcgccgccag
     4681 tccgagcacg gtgctggtgc agggcgagac cggtaccggc aaagagctgg tcgcccggac
     4741 gctgcacacg ctgagtccgc gcgcccaggc gccgttcgtg gcggtcaact gcggcgcgct
     4801 gcccgacacc ttgttggagt cggagttgtt cggatacaag gccggggctt tcaccggcgc
     4861 gacacggaac aagcccggac gcttcgcgct ggccaagggc ggcacgctct ttctcgacga
     4921 gatcggcgag atcagtcctg cgttgcaggt gcgcctgctg cgggttttgc aggagcgggt
     4981 ctacgagccg ctgggggcca cccgttccga gaaagccgat gtgcgcgtgg tcgtggcgac
     5041 caaccgcgac t
//
LOCUS       DAOSRB010000195         4983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15340_length_4983_cov_5.292411, whole genome shotgun sequence.
ACCESSION   DAOSRB010000195 DAOSRB010000000
VERSION     DAOSRB010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15340_length_4983_cov_5.292411"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="PLZ74_09320"
     CDS             <1..1625
                     /locus_tag="PLZ74_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98561.1"
                     /translation="TNHLLSFTPQPDGIALVLSNSVSGVVSTHTALMLDLAPWDASEW
                     VFTPATSLMLVTEILPVAPLPMPETLSTSLAVLLSLLPTETLNGLYSFLLLRPDEFND
                     EAWGALFSYLNLNPLVLRTLTPSRREPTLGNMTEKDRFPLTDGTGTPQPYESDESGRV
                     DVTGFRTDGFGRYWTVYPKKTVAFPEITIIEADQSVSGQREVITNYIALGKNGNRLWL
                     QPTVTINTNRSTAMSGDAIVDEALLTRGGESVQGWSAVTNLSIADPRHNAYAAFWRPV
                     HSLSAWKDELGTTNRSTEVTEYPFIQLNTPLHSIADLGHVYTSPDRLRLTHTFLLGNA
                     TASPDLNDKLPRHDTIAFVSRSGAGLLDVFTIHPTNRPPWRGLVQANTHHEPVIQTLF
                     SFGKLGWTNTTEGGKSFLSDLSDVSDLSDWSDAFRDALANTPPTGIGWRSFADMLPAI
                     STNELLRTSFTPPFNDAPAHDWIEDAVRHLPDLVSFRQNIFVIVIAAQTLSPASTPSR
                     PVVLADQRAAVTVIRDAFTGRWTVHDWRWLTE"
     gene            complement(1734..2192)
                     /locus_tag="PLZ74_09325"
     CDS             complement(1734..2192)
                     /locus_tag="PLZ74_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98562.1"
                     /translation="MNQLCFAACVALMLGLSVRAEEKPAAKQEGFPKRANSRAGLIQK
                     FDKDGDGKLNEEERAAMREEMRKNREKAFDKNGDGKLDDTERAAMQEARKAREKAFDK
                     DGDGKLSIEERKAMREELMKNRRGGGPGKGRPDKGKKKGPPPAAPAAPGA"
     gene            complement(2300..3565)
                     /locus_tag="PLZ74_09330"
     CDS             complement(2300..3565)
                     /locus_tag="PLZ74_09330"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOR98563.1"
                     /translation="MPEKTLPLSPAEMERLTQRYPTPFHLYDERGIRENARRLQAAFA
                     WNPGFREYFAVKACPNPFLMAILSEEGFGMDCSSLPELLLSERVGVTGEKIMFTSNNT
                     PAEEFVKARELGAIINLDDLAHIPFLEASAGVPERICLRYNPGPLKGGNAIIGRPEQA
                     KYGFTREQLFEGYRQLKAKGVRHFGLHTMVASNELNPQYFIDTAEILFDLVCELSREI
                     DVPFEFVNIGGGIGIPYRPEQEAVSLETVGEGIRRAYASRLLAKGHPELNLYMECGRM
                     ITGPYGYLVTRVRHIKRTYKTYAGLDACMANLMRPALYGAYHHISVPAKAGAPCDTVY
                     DVTGSLCENNDKFAIDRPLPVLEPGDLVVIHDAGAHGHAMGFQYNGKLRSAEVLLRED
                     GGTLLIRRAETPEDYFATLDFEALKGFRA"
     gene            complement(3616..3692)
                     /locus_tag="PLZ74_09335"
     tRNA            complement(3616..3692)
                     /locus_tag="PLZ74_09335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3656..3658),aa:Arg,seq:cct)
     gene            complement(3890..>4983)
                     /locus_tag="PLZ74_09340"
     CDS             complement(3890..>4983)
                     /locus_tag="PLZ74_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98564.1"
                     /translation="TAQVAPSARPAEPAAPVAQKSDPAALRAKEAAEAATRVEGAAAR
                     LRKEGLAACASVLDTLAAVPIPLRAEETLKTKWVAVRDASAELLEREILRKDPLAQRA
                     QRLKTAGEIVQGRAIEAVMEASRVAELSKSLQQQQGVCVLRLVNQGTQTVQVSAREGL
                     EQTSLAGGEVKDWQIPVSGETLTMSITVDEGNRFRSRMETVRLPRGGGVERAIGSAVA
                     QTAAPTPKPEAAPAAKPEPVTPSPPATQPVAAPSPVVAAEKTAPAAPSPAAVAEKTPQ
                     TAPAAPAPAAAKAFFQLSVSPKIGVLMVDGKVVDAGLVEIEPGVNHKVEAVCQGYKRI
                     EQYYKAKPGETRKIDILLEKEVKRSFFGF"
BASE COUNT          905 a         1715 c         1393 g          970 t
ORIGIN      
        1 tcaccaatca cctgctctcg ttcacccccc agcctgacgg cattgcgctt gtcctcagca
       61 acagcgtttc cggggttgtg tccacccata ccgccctgat gcttgatctc gcgccgtggg
      121 acgcctccga atgggtcttc acgccagcca cgtctctcat gctggtaacc gagatcctcc
      181 cggtcgcgcc gctccctatg ccggaaacgt tgagcacatc tctcgccgtt ctcctctccc
      241 tgctgcccac ggaaacactt aacggacttt actccttcct gctcttgcga cccgatgagt
      301 ttaacgacga agcgtggggg gcattgttca gctatctgaa tttgaacccg ctcgttctcc
      361 gcacgctcac accgtcgcga cgcgaaccca cgctcggcaa catgaccgaa aaggaccgct
      421 tcccgctgac cgacggcacc ggaaccccac agccttatga gtcagacgaa agcggccgtg
      481 tcgatgtgac gggtttccgt accgacgggt tcggccgcta ctggaccgta tatccgaaaa
      541 agactgtcgc cttcccggaa atcaccatca tcgaggctga ccagtcggtc tctggccagc
      601 gcgaggtcat cacgaactat atcgctctgg gcaaaaacgg gaaccggctt tggttgcaac
      661 cgacggtgac catcaacacc aaccgctcga ctgccatgtc gggtgacgcg attgtggatg
      721 aggccctgct cacccgaggc ggggagagtg tacagggctg gtcggcggta acgaatcttt
      781 ccatcgcgga cccgcggcac aacgcctatg ccgccttttg gcgacccgtg cattccctgt
      841 cggcctggaa agacgaactc ggcaccacca accgcagcac tgaggtcacg gagtacccct
      901 tcatccagct gaacacgccg ctccactcca ttgcggacct cggccatgtg tacacctcac
      961 ccgaccgcct gcgcctgacc cacaccttcc tgctgggcaa cgccacggct tcccccgacc
     1021 tgaacgataa actcccgcgt cacgacacca tcgccttcgt ctcccgttcc ggcgccgggt
     1081 tgctcgacgt gttcacgatc catcccacca accgaccgcc ttggcgcgga ctcgtgcagg
     1141 ccaacacgca ccatgaaccc gtgatccaga cactcttcag cttcggcaag ctcggctgga
     1201 ccaacactac cgaaggggga aagagttttc tatccgacct gtccgacgtg tccgacctgt
     1261 ccgactggtc cgacgccttt cgcgatgcgc tcgccaacac gccgcccacc ggcatcggct
     1321 ggcgctcgtt cgcggacatg ttgcccgcga tctcgacgaa cgagcttctg cgcacgtcct
     1381 tcacgccgcc gtttaacgac gccccggccc acgactggat cgaggacgcc gtgcgccacc
     1441 tgccggacct ggtctccttc cgccagaaca tctttgtgat cgtgatcgcg gcgcagacgc
     1501 tctcgcccgc ctccacgccg tcgcgcccgg tggtgctcgc ggaccagcgc gccgccgtca
     1561 ccgtgatccg cgacgccttt accggccgct ggaccgtcca cgactggcgc tggctcacgg
     1621 aatgatgccg aaaacgcggc ggggaagaaa gagacggccc gttacagaaa aacccggctg
     1681 cggaggctaa accttcgcaa accgggctca tgttcctctc tgagcttacg cgtttacgcg
     1741 ccgggcgcgg cgggcgcggc gggcggcgga ccctttttct tacccttgtc gggcctgcct
     1801 tttccggggc caccaccccg gcggttcttc atgagttcct cgcgcatcgc cttgcgctcc
     1861 tctatgctca gcttaccgtc cccgtctttg tcgaacgcct tctcgcgcgc cttgcgggcc
     1921 tcttgcatgg cggccctttc ggtgtcatcg agtttgccgt cgccgttctt atcgaacgcc
     1981 ttctcgcggt tcttgcgcat ctcctcgcgc atcgcagccc gctcctcctc gttcaacttg
     2041 ccgtccccgt ccttatcgaa cttctggatc aagcccgccc tggaattcgc ccgtttgggg
     2101 aacccctcct gtttggcagc gggcttctcc tcggcccgaa ccgacagacc cagcatcagc
     2161 gccacacatg cggcgaaaca gagttgattc ataacatgac tccttgatta cggttgtcgt
     2221 gtacaacgga caacgtctgg tcgtaaaaaa cggccccgtg tccgctttat tgccggcccg
     2281 tgccgattcc ccatccggct caggcccgaa atcccttgag agcctcgaaa tccagcgtgg
     2341 cgaagtagtc ctcaggcgtt tccgcgcgcc ggatcagaag cgtgccgcca tcctcgcgca
     2401 acagcacctc ggccgaacgc agcttcccgt tgtattgaaa acccatggca tgcccgtgcg
     2461 cgcccgcgtc atggatcacg acaagatccc ccggctccaa aaccggcagg ggacggtcga
     2521 tcgcgaactt atcgttgttt tcgcacagcg acccggtcac gtcgtacacc gtgtcgcacg
     2581 gggcacccgc tttggcgggc acggagatgt gatggtacgc gccgtagagc gcaggccgca
     2641 tcaggttcgc catacaggca tcaaggccgg cataggtttt gtaggtccgc ttgatgtggc
     2701 gcacacgcgt gaccagatac ccgtacggtc cggtgatcat ccggccgcac tccatataga
     2761 gattgagttc aggatggcct ttggccagca ggcgcgaggc atacgcgcgc cggattccct
     2821 cacccacggt ttccagactc acggcctcct gttcgggccg gtacgggatg ccgatgccgc
     2881 cgccgatgtt cacgaattcg aacgggacat cgatctcgcg agacagctcg cacaccaagt
     2941 caaagaggat ctctgcggta tcgatgaaat actgggggtt aagctcgttc gacgccacca
     3001 tcgtgtgcaa cccgaaatgc ctcacgccct tggccttcag ttgccggtac ccctcaaaaa
     3061 gctgttcgcg cgtgaacccg tatttggcct gctcgggacg gccgatgatc gcattgccgc
     3121 ctttgagcgg accggggttg taccgcaagc agatgcgctc cggaacgccc gcgctcgcct
     3181 ccagaaaagg gatgtgcgcc agatcgtcga ggttgatgat agccccgagt tcccgcgcct
     3241 tgacgaactc ttcggcaggc gtgttgttcg acgtgaacat gatcttctcg cccgtaaccc
     3301 cgacgcgttc ggaaagcagc aattccggca atgagctgca atccatgccg aacccctcct
     3361 cgctcaagat cgccatcagg aacgggttgg gacaggcctt gaccgcaaag tactcccgga
     3421 atccggggtt ccaggcgaac gccgcctgaa ggcggcgcgc gttttcgcgg ataccgcgct
     3481 catcgtacag atgaaagggg gtaggataac gttgcgtcag gcgttccatt tcggcaggcg
     3541 acagcggcaa agtcttttcc ggcataaact gcggctccac acgtcattcc gacggattca
     3601 aacaagacag caaaatggta ggccaggccg gagtcgaacc ggcgaccccc agattaggaa
     3661 tctagtgctc tgtcctactg agctactggc ctttaaacgg ttagtgataa cataacgggg
     3721 tgatctctgg caagcccgcg acgcagagcc tacgcacttc caacacgggc ggtaccccac
     3781 aaccaaattt cacctcacat aacggcacaa aggacacaac gtgtttttca tgacgcccca
     3841 ttctccttca tgccttttgt gtcttccatg agaaacaact tttttcttat cagaacccga
     3901 agaacgaccg cttgacctct ttctccagca ggatgtcgat cttgcgcgtt tcaccgggct
     3961 tggccttgta atactgctcg atccgcttgt agccctgaca caccgcctcg accttgtggt
     4021 tcacgccagg ctcgatttcg accagaccgg catccaccac cttgccgtcc accatcagga
     4081 ccccgatctt gggcgacacc gaaagctgga agaacgcttt ggcggccgca ggcgcaggag
     4141 ccgctggcgc ggtctgcgga gttttctcgg ccacggcagc gggagaaggc gcggcgggcg
     4201 cggttttctc agccgcaaca acgggggacg gcgcggctac cggttgagtg gcgggcgggg
     4261 aaggagtgac gggctcgggc ttggcggcag gcgcggcctc gggtttgggc gtgggtgccg
     4321 ccgtctgcgc gaccgcgctg ccgatggcgc gctcgacccc gccgccgcgc ggcagccgga
     4381 cggtctccat acgcgaacgg aaccggttgc cctcatccac cgtgatcgac atcgtgaggg
     4441 tctctcccga caccgggatc tgccaatcct tgacctcgcc accggccagc gacgtctgtt
     4501 ccaacccctc gcgcgccgag acctgtaccg tctgcgtgcc ctgattgacc agccggagca
     4561 cacacacgcc ctgctgctgt tgcaggcttt tgctcagttc cgccacgcgc gaggcctcca
     4621 tcacggcctc aatggcccgg ccctgaacga tctccccggc ggtcttcagc cgctgtgcgc
     4681 gttgcgcgag cggatctttc cgcaggatct cccgctccaa caattcggcg ctagcgtccc
     4741 gcacggcaac ccacttggtt ttgagagtct cctccgcacg cagcggaatc ggaactgcgg
     4801 cgagcgtatc caacacgctc gcgcaggctg cgaggccctc tttccggaga cgcgccgccg
     4861 caccttccac ccgcgtagcc gcctcagccg cttctttggc gcgaagcgcg gccggatcgg
     4921 atttttgggc gaccggagcg gcaggctcgg caggccgggc agaaggtgcg acctgagctg
     4981 taa
//
LOCUS       DAOSRB010000196         4976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15372_length_4976_cov_6.675676, whole genome shotgun sequence.
ACCESSION   DAOSRB010000196 DAOSRB010000000
VERSION     DAOSRB010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15372_length_4976_cov_6.675676"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLZ74_09345"
     CDS             <1..257
                     /locus_tag="PLZ74_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR98565.1"
                     /translation="YGIPYRCLLPLKTTNVLIAGRAASFSAIAASSCRLSRTMMQLGQ
                     AAGTAAHLSCSGNIPLREVQASEIRRVMAEHIDQFVQKCE"
     gene            457..2205
                     /locus_tag="PLZ74_09350"
     CDS             457..2205
                     /locus_tag="PLZ74_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR98566.1"
                     /translation="MLTVFRAPAQTAVSNVVILIEATPLASLVRPGPEPLRDIPGLML
                     RQQGASAPQGDLSIRGSPFNGAGLLLNGLTLRNAQTEHWHADLSVPNIWTDTPTVLTG
                     MERFRAASGHPSGSVSLELAPLSETLRSITLGGGDKGALFGQAVFTETEPFRDGVAGA
                     SAFVSADRADQTDGYRDNDLVRGAAGARIHAANETMRSDLVTALSWREFGARGFYGTD
                     PSFPAAERKGDALAAGSLRFVQDPRQPARLSAAWLRTRDTYWLDRHNRTFYENDHTTD
                     FFVAHGDTRRTFNDTVSVDLRADGDLETLESRSLGNHTRSHASLAVLPNLTCGPLTWT
                     VGGALDLFSTDSPIPLPAAGVEWTVSERHTLFLSYTAAARQPSYTELNYESPSSLGNA
                     GLDRQRTQTAELGWKAEHGPFRWRTVLFHEEGRNIVDWRRTSPAGRWQAVNLETLRTT
                     GLAAEGVWEVDGNTDFGLNLLTVSKDTDTSGYASRYALDYPRAAADACVRHRLTRELS
                     LRLSQGVSRYATNHARQHDNWFWDTRAGVQWQPRQLPGLTLNAGIDNLLDDDFQIFPG
                     QETAGRCMYVSLTHVW"
     gene            complement(2227..2805)
                     /locus_tag="PLZ74_09355"
     CDS             complement(2227..2805)
                     /locus_tag="PLZ74_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98567.1"
                     /translation="MRSEAAAKQSFGATAYAVRPGVCVACGRAAGRHERACVYCGERV
                     WRSFRNRAARAAWIAVPPVLLLWLTVAARPDWGAVCQTILDGGALSGYLFAAGAGLLL
                     LPLEDDDLVTASETERLQWQALSHAGAFLCGGYAALGSACLACGARVGPAVWVAVIAV
                     FACVALAPLLFRISWRAVAAAALWAAVWLGHG"
     gene            complement(2865..4664)
                     /locus_tag="PLZ74_09360"
     CDS             complement(2865..4664)
                     /locus_tag="PLZ74_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOR98568.1"
                     /translation="MPMSRMSFHRVAICFALLLATRAILAPAAPFLPLSLTDAANTGF
                     SDVEEGDRKGGWLDLGGNDLSVLPAGLLTVSSRLPFQILDSKKNPDRTCVVLGGPKRP
                     YLPSRAEIRVDGKTGAVLYLLHGAAWCPPPQQQKMAGVLTVEYADGTGKEYHVRCGRD
                     VGDWTKPDSYSNAARVWSAYNANTQVSLFASKFKLEAKPIRLIRLRSQECAWMVVAAT
                     LGDEISIKPIRRNLTLDKKYTAPPPFEKPLRDDLSDTRPKNIILIIGDGMGEGAQKLT
                     SLYQHKAEGRLAMQQLPVTGYLTTFSANSEVTDSAASSTAIACGHKTNNGMLGLTPQT
                     NTVLSVAALARESGRAVAILTSDAVTGATPSGFYAHVVRRGAYSDIAEFAAACGYDML
                     IGNANARAWFTPEAAGGQRTDTRNVLNEMTAAGYAVVTNAALFAAVPEGQRVVGFLDK
                     PTLEPETALGTLMDIALPRLARHDKGFFMMMECTITDAGGHGNNPEYTVRGTLQVDWA
                     VRRAVDFAKSRGDTLVLVTADHETGGLFCSLSRAVPDRLTICYTTTSHTGAPVPIHAF
                     GPGSKYFQGMNDNTDIAKTVAFLWRMRLSAPTR"
BASE COUNT          913 a         1722 c         1432 g          909 t
ORIGIN      
        1 cctacggcat tccgtaccgt tgtctgttgc cgttgaaaac cacgaatgtc ctgatcgccg
       61 gacgggccgc cagtttcagc gccatcgcag catcaagctg ccgcctttcg cgcaccatga
      121 tgcaactcgg gcaggcggcc ggcaccgccg cgcatctgtc atgttcaggc aacatcccgc
      181 tacgcgaagt gcaggcatct gagatccggc gcgtgatggc ggagcatatc gaccaatttg
      241 ttcaaaagtg cgaatgaaca taacaaaaaa agcaaccgac taacgagtct cttccgcctt
      301 ctcctgcggg ctttgcccgc cccggctttc tgctcgcccc catattgtaa cctattttat
      361 ttcttgtggt tgacaatcat tcccggcccg gttaatctcg cgtctctcat gcacttcccc
      421 cggttagtct tacgccccct ccccgtattg tttgtgatgc tcaccgtgtt ccgtgccccc
      481 gctcaaactg ccgtctccaa tgtagtgatc cttatcgagg ccacaccctt ggcctccctt
      541 gtccggcccg ggcccgaacc gttgcgcgat atccccggtc tcatgcttcg ccagcagggc
      601 gcaagcgcgc cgcagggtga cctctcgatc cgcggcagcc cgtttaacgg tgcggggttg
      661 ctgctgaacg gcctgacact gcgcaatgcc cagacagagc actggcacgc ggacctgtcc
      721 gtgccgaaca tctggaccga caccccgacg gtgttgaccg gcatggaacg tttccgcgcc
      781 gcgtccggcc acccctccgg ctcggtgagt ctggagctcg cgcccctctc cgaaaccctg
      841 cgctccatca cactcggcgg cggcgacaag ggcgctcttt tcggtcaggc ggtcttcacc
      901 gaaaccgagc ctttccgcga cggcgttgcc ggtgcctcgg ccttcgtttc agccgaccgg
      961 gccgaccaaa ccgacgggta tcgcgacaac gatctcgtac gcggtgcggc cggtgcccgg
     1021 attcatgctg ccaacgagac gatgcgaagt gatctcgtca ccgcgctctc ctggcgcgag
     1081 ttcggcgcgc gcggctttta cggcactgac ccgagttttc ccgccgcaga gcgcaagggc
     1141 gacgcgctcg cggccggatc acttcgtttc gtgcaggacc cgcgccaacc ggcccgcctc
     1201 tccgccgcct ggctccgcac acgggacacc tattggcttg accggcataa ccggacgttt
     1261 tacgagaacg accacaccac cgattttttt gtcgcgcacg gcgacacgcg ccgcacgttc
     1321 aacgacaccg tctccgtaga cctgcgcgcc gacggggatc tcgaaacgct ggagagccgc
     1381 tcgctcggca accacacccg cagccacgcc tcgctcgccg ttctgccgaa tctcacctgc
     1441 ggtccgctca cctggaccgt gggcggagcg cttgatctct tctccaccga ctcccccatc
     1501 cccctgcctg ccgcaggtgt cgaatggacc gtttcggaac gtcacactct gtttctgtcg
     1561 tacaccgccg cagcccgcca accctcatac accgaactca actacgaatc gccttcttcc
     1621 ctcggcaacg ccggtctcga ccggcagcgt acccaaaccg ccgaactcgg ctggaaggcc
     1681 gagcacggac ctttccgatg gcgtacggtg ttgtttcatg aagaggggcg gaatatcgtg
     1741 gactggcgcc gcacctcgcc cgcaggccgc tggcaggcgg tcaatctcga aactctgcgc
     1801 accacgggcc ttgccgcaga gggcgtctgg gaagtggatg gtaacacgga tttcggactt
     1861 aacctgttga ccgtgtccaa ggatacggac acgtccgggt atgccagtcg ttacgcgctg
     1921 gattatcccc gcgccgccgc cgacgcatgc gtacgccacc gtctgacgcg cgagctttct
     1981 ttgcggttga gtcaaggagt cagccgttat gccaccaatc acgcccgcca gcatgacaac
     2041 tggttttggg acacccgcgc aggggtgcag tggcagccgc gtcagctacc cggcctgacc
     2101 ctgaatgccg gcatcgacaa cctgctggac gatgatttcc agatctttcc cggccaggaa
     2161 accgcagggc gctgcatgta cgtctccctc acccatgtct ggtaagtccg gcacaaccgc
     2221 cgttcgtcat ccgtgtccga gccacaccgc agcccaaagc gctgcggccg ccaccgcgcg
     2281 ccaagagatc cggaagagaa gaggcgcgag tgcgacgcaa gcgaatacgg cgatgaccgc
     2341 aacccagacc gcaggtccga cgcgcgcgcc acacgcgaga cacgccgacc ccaacgctgc
     2401 ataccccccg cacaggaacg cgcccgcatg tgagagggcc tgccattgga gtcgttccgt
     2461 ctccgacgcg gtcaccagat catcatcctc cagcggcaac agcagcaggc ctgcccccgc
     2521 tgcgaacaga tatcctgaaa gcgcaccgcc gtcgaggatg gtttggcata cggcacccca
     2581 atcgggccgg gccgcaaccg tgagccacag caacaacacg ggcggcacgg caatccacgc
     2641 cgcgcgcgca gcgcgattgc ggaatgaacg ccaaaccctc tcgccacaat agacgcaggc
     2701 acgttcgtgc cgaccggccg cacgtccgca cgcaacgcat acccccggcc gtaccgcgta
     2761 cgccgtcgct ccgaaactct gttttgcagc ggcctcagac ctcatcgcag agaaattccc
     2821 ttgccgctcg gtgcggacgc aaaaggaaca ccggtgtcgc ctcctcaccg cgtgggcgcg
     2881 gacaggcgca ttcgccacag gaacgccacg gttttggcaa tatccgtgtt gtcgttcatg
     2941 ccctggaaat acttggagcc cggaccgaac gcatgaatcg gaaccggtgc gccggtatga
     3001 ctggttgtgg tgtaacagat cgtcagtcgg tccggcacgg cacggctcaa cgaacagaac
     3061 aggccccccg tctcgtggtc ggccgtgacc agcaccagcg tgtcgccgcg cgacttcgcg
     3121 aaatccacgg cccgccgcac cgcccagtcc acctgcagcg tgccgcgcac cgtgtattcc
     3181 ggattgttgc cgtgtccgcc agcgtcggtg atggtgcact ccatcatcat gaaaaacccc
     3241 ttgtcgtggc gggcgagacg cggcagcgcg atatccatca gagtccccag cgcggtttcc
     3301 ggctccagcg tgggcttatc cagaaaaccg accacccgct gcccctcggg cacggcggcg
     3361 aacaacgccg cgttcgtgac cacggcatat ccggcggcag tcatctcgtt cagcacgttc
     3421 cgggtgtcgg tgcgctggcc acccgccgct tcaggcgtga accacgccct ggcgttggcg
     3481 ttgccgatca gcatgtcgta accgcaggct gcggcgaact cggcgatatc ggaatacgcg
     3541 ccgcgccgca cgacgtgcgc gtaaaatccg gagggcgtgg cgccggtcac cgcatcggag
     3601 gtgaggatcg ccaccgcgcg tccgctctcc cgggccagag ccgccacgga aagaacggtg
     3661 ttggtttgcg gcgtcaggcc caacattccg ttgttcgtct tgtgcccgca ggcgatggcg
     3721 gtggaggacg cggccgagtc cgtgacctcc gagttggcgg aaaaggtggt gaggtagccc
     3781 gtgaccggca actgctgcat ggccagacgc ccctccgcct tgtgctgata aagcgaggtg
     3841 agtttttgcg cgccttcgcc catgccgtcc ccgatgatca ggatgatgtt cttcggccgt
     3901 gtgtcactca aatcgtcccg caagggcttt tcgaacggcg gcggcgcggt atatttcttg
     3961 tcgagcgtca ggttgcggcg gatcggcttg atcgagatct cgtcgcccag tgtggccgcc
     4021 accaccatcc aggcgcactc ctgcgaacgc aaccggatca gccggatggg tttggcctcc
     4081 agcttgaatt tggaggcgaa aagtgacacc tgggtgttcg cgttgtacgc gctccagaca
     4141 cgcgcggcgt tcgaataact gtccggcttt gtccagtcgc cgacatcgcg gccgcaacgg
     4201 acatgatact ctttgcctgt cccgtccgca tactccacgg tcagcacacc ggccatcttc
     4261 tgttgctgcg gcggcggaca ccaggccgcg ccgtgcagga ggtagaggac cgcgccggtt
     4321 tttccatcca cgcggatttc ggcccgcgac ggcagatagg ggcgcttggg gccacccagc
     4381 accacgcagg ttcggtccgg atttttcttg ctgtccagaa tctggaacgg cagacgggac
     4441 gaaaccgtca acagacctgc gggcagcaca ctcagatcgt tcccgcccag atcgagccat
     4501 ccgcccttgc ggtcgccctc ctccacatca ctgaagcccg tattcgccgc atccgtgagc
     4561 gacagcggaa ggaacggagc cgccggagca aggatcgctc ttgtggcgag aagcaacgcg
     4621 aaacagatgg caacacggtg aaacgacatc cgtgacatcg gcataatgat cccctcttcg
     4681 tctgggtgcg ccctcttccc cggctccccc ttttatgagc cggatccggc gcactccggt
     4741 tggtttaaca aaacagggtc agtctagcag gaaccgcggg aacgcggaac cctttttttc
     4801 ggatcgccca tgccccggaa cacctccccg atgcggatca tggccaagcc aatgagtacg
     4861 cacctttgtc caaaacccat ctctttggtt tcagggtttc atccaaatcg ggatcgggat
     4921 cgctatcggg atcgggatcg atcatttaac cacggaatac caccgcctca cagcct
//
LOCUS       DAOSRB010000197         4965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15408_length_4965_cov_5.473727, whole genome shotgun sequence.
ACCESSION   DAOSRB010000197 DAOSRB010000000
VERSION     DAOSRB010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15408_length_4965_cov_5.473727"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3263
                     /locus_tag="PLZ74_09365"
     CDS             <1..3263
                     /locus_tag="PLZ74_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98569.1"
                     /translation="SGECCFLPRMSAEAQTGTWQTFAGSPILLRAGAAHDLAAGATVS
                     GEGIPEGAFVKCIYSNSAIELSVNCTADSGESGVAVNFGAFQNVRTVQHITRLQNQAT
                     GNSVLSHYFSKYRAEDLFDVYVDDIGGRVDLSMVPNNQRSFATNQVAYYPARIFLKSA
                     AAVGTGHIRIRNTHLVFNPEDGATFSLNAADLGSSVAFEAWGPRLETPAGVTATIQDI
                     LRWTLTVTKDGEGTLDVTSTNLAHNALIAEKGLTILRMTAPTANQTLASLAVSNGAAF
                     KLAEGTLAVSSATLGSGATLSVDEGAVLDLSGGALFAGVTLQGPGTFIVKDVSVLSGA
                     VIEGTPVFRFANAGTARDLAPVGEVIPAVVGHPAFWVDAAKNVDLITFEWEGVTNENG
                     VACWRDCRDGETTYYATNDFSQLTVQTGVPSRYEANLYNGLPCVWHDGIATTNITNFR
                     NVQSGMVWNQPITNVCAVFHVVYNQDNGGGNAVLGCTKRFAEMGTARTYNDFGRGIYS
                     VTDGGGVGTWQNYLLAAGVPTWVRNGWLYVDGQEWDLAAKMPAAYSVPYVLEVHPLAP
                     YGRADAFAIQEHAGVTCSGCQRQMECIVYTNELTLTERRQVCAYLMQKYRNATVEYNL
                     SDSRFTNRSNLDALDVTHDVGVDVAAGNVMGFTTLTNSATLMKTGGGTLYADLVDGAS
                     LDVREGEVVIRSQELTADVVPSGPFVHLDATATNRMTLTSKTYLDGATRDEVTHWRDV
                     SGASPVSAQRRTTDDRYKGPYLSYPESLGGRPVVDYGVLTNTSPVDFNYGWGLFHDLV
                     WQETDGYTPMFYTNLTTLGSVFLMIGSQNGGGVPLGGYGTAQWRTATADPAVPFFNSN
                     APTGCRSGGNWLNGVRTTFTTAGLSGGYDLVSFRDTVGTGCNVQSLMMANARQFSGGG
                     ELGEVLIYEEPLSYYTHQVVDAWLNWKWFARKTVGHAPSAAEDVTLAAGTTLTVEGDM
                     PLTVTRLAGAGTINGSVAFASGAGLEVVINDNGTITPLTVSGAVDLSDGGTVTLKGDI
                     SKLATGSYPLVFCTSAIAGPWNVQYTGTSYLKYFVRIRPDGIYLDVVSPGTLVIFR"
     gene            complement(3304..4458)
                     /locus_tag="PLZ74_09370"
     CDS             complement(3304..4458)
                     /locus_tag="PLZ74_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98570.1"
                     /translation="MKKRIVIGTALISLAGYASFPPDAVLSAFPRLAGEHDDAPRFRR
                     AIAAAPNGVLAVGKGDYSIATPLVITNRCSLEMHPAARLIATAPMEFVLTWDGHDDYH
                     ALSIFNPDGSIYDNAGLFIRGGDIDGRGLASCLRIGNAHHFTLANITLHNGRKAGLEL
                     SRWNGGHLYELIANNVYCKCTMKGLAGNIGVLVQVHDCHFTDIIVVDYTIGIRATGSS
                     NRFTRCHVWGGTVAPKGMSMREWSDFYAANKKQRSRWNADLEAALLSKGLPEMLAGSI
                     CFDSSSGNLFDGCYADTGEIGFNLPHGGQLVNCGFYNNLLMGLRKSTVIVHKSGRLVI
                     TGCKFNGVTGVEKLYEGPWKDVVWSSSTASGGADMDADATKLRHGTPVQK"
     gene            complement(4663..>4965)
                     /locus_tag="PLZ74_09375"
     CDS             complement(4663..>4965)
                     /locus_tag="PLZ74_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR98571.1"
                     /translation="MATRIAVMYAGQIVEQAASEAFFRTPLHPYSIALLEAMPSAATR
                     GKRLKAIPGQVPPAGNFPPGCRFHDRCPVARPECARTPPALRVLEDRSVRCPYAGA"
BASE COUNT          951 a         1488 c         1567 g          959 t
ORIGIN      
        1 gttcgggtga gtgctgcttt ctgccgcgca tgagcgccga ggcgcagacc gggacgtggc
       61 aaacctttgc cggttcgccg attctccttc gcgcgggagc ggcgcacgac ctcgcggccg
      121 gcgcaacggt ttccggtgag ggcatccccg aaggggcgtt cgtgaaatgc atctattcga
      181 acagcgcgat cgaactctcc gtgaactgca cggctgattc gggggagagc ggtgtcgcgg
      241 tcaacttcgg cgcgttccaa aacgtgcgga cggttcagca cattacgcga ttacagaatc
      301 aggcgacagg aaactcggtg ctgagtcact atttctccaa ataccgtgcc gaggacctct
      361 tcgacgtgta tgtggacgat atcggtgggc gcgtagacct ctcgatggtg ccgaacaacc
      421 agcgttcctt tgcgacgaat caggtcgcct actatcccgc gcgcatcttt cttaaaagcg
      481 ctgcggcagt cggcacgggg catatccgga ttcggaatac gcatctcgtc ttcaatcccg
      541 aggacggcgc aaccttctcc ctgaacgctg cggatctcgg gagttccgtt gcctttgagg
      601 cttgggggcc gcggctggag acgcccgctg gcgtcacggc gacgatccag gatattttgc
      661 gctggacgct gaccgtgaca aaagacggtg agggcacgct cgacgtcact tccacgaatc
      721 tcgcgcacaa tgcgctgatt gcggaaaagg gcctgaccat tctgcgtatg acggcgccga
      781 ccgcgaacca gacgctcgcc tcgctcgccg tttcaaacgg cgccgcgttc aaactcgcgg
      841 aaggtacgct cgccgtctcc tcggcgacgc tcggttccgg tgcgacactg agcgtggatg
      901 aaggcgccgt cctcgacctt tcggggggtg cgttgtttgc gggcgtcaca ctccagggcc
      961 ccggcacatt catcgttaaa gacgtctctg tcctttccgg tgcggtgatt gaaggcacgc
     1021 ctgttttccg gtttgcgaac gcgggcacag cgcgcgatct cgctccggtg ggcgaggtca
     1081 ttcccgcagt ggtcggccat ccggcgttct gggtggacgc tgcgaagaac gttgatctga
     1141 taaccttcga gtgggaaggc gtgacgaacg aaaacggcgt cgcctgctgg cgcgactgcc
     1201 gggacgggga aaccacgtat tacgcgacaa acgatttttc gcagttgacc gtgcagacgg
     1261 gcgtgcccag ccggtacgag gcgaatctgt acaacgggtt gccgtgcgtg tggcacgacg
     1321 ggatcgcgac gacgaatatc accaactttc ggaacgtgca gtccggcatg gtgtggaacc
     1381 agccgatcac gaatgtctgc gccgtgttcc atgtcgtgta caaccaggat aatggcggcg
     1441 ggaacgcggt tctcggctgc accaagcgct tcgcggaaat ggggacggcg cgcacctaca
     1501 acgacttcgg acgggggatc tactccgtca cggacggcgg cggtgtcggc acgtggcaga
     1561 attaccttct ggcggccggc gtgccgacgt gggtccggaa cggctggctg tacgtggacg
     1621 gtcaggagtg ggatctcgcc gcgaagatgc ccgccgcgta ctcggtgccg tatgtgctcg
     1681 aagtacatcc tttggcgccg tacggccgcg ccgatgcctt cgccatccag gaacacgccg
     1741 gcgtcacctg ctccggctgc cagcgccaga tggagtgcat cgtctacacg aacgaactta
     1801 ctttgacgga gcggcggcag gtgtgcgcgt acctgatgca aaagtaccgt aatgcgacgg
     1861 tcgagtacaa cctttccgat tcgcgtttca caaaccggtc gaatctcgac gctttggacg
     1921 tgacccacga tgtgggggtt gacgtggctg ccggcaacgt gatgggcttc accacgctca
     1981 cgaacagtgc gaccctcatg aaaaccggcg ggggcacgct ttacgcggat ctcgtggacg
     2041 gcgcttcgct cgacgtccgc gaaggcgaag tcgtcatccg ttcgcaggaa ctgaccgcgg
     2101 atgtggttcc gtcgggaccg ttcgttcatc tcgatgcgac ggccaccaac cgcatgaccc
     2161 tgacctcgaa aacgtatctc gacggcgcga cgcgggacga ggtgactcac tggcgcgacg
     2221 tgtccggcgc ctcgcccgtg tccgcgcagc ggcgcactac ggacgaccgg tacaaggggc
     2281 cgtacctctc ctatccggag agtctcggcg gcaggccggt ggtcgattac ggcgtgctca
     2341 cgaacacctc gcccgtcgac ttcaactacg gatggggcct cttccatgat ctcgtctggc
     2401 aggaaacgga tggttacact ccgatgtttt acaccaacct gaccacgctt ggcagcgtgt
     2461 ttctgatgat cggctcgcaa aacggcggcg gtgtgccctt gggaggttat ggtacagcac
     2521 agtggcgcac tgcgaccgca gatccggcgg tgccgttctt caattccaac gcgccgacag
     2581 gctgccgttc cggcggcaac tggttgaacg gcgtgaggac aaccttcacg accgcggggc
     2641 tttccggcgg gtacgatctg gtctctttcc gcgataccgt gggaacgggg tgcaacgttc
     2701 agtcgctcat gatggcgaac gcgcgtcaat tcagcggcgg tggtgaactg ggcgaggtcc
     2761 tcatctacga agagccgctc tcgtactaca cgcatcaggt cgtggatgcg tggctgaact
     2821 ggaagtggtt tgcgcgcaaa acggtgggtc acgcgccgtc cgcggccgaa gacgtgacgc
     2881 tggccgctgg cacgaccttg acggtggagg gtgatatgcc gcttacggtg acgcggctcg
     2941 ccggcgccgg cacgattaac ggctcggtgg cgttcgcctc cggcgccgga ctggaggtgg
     3001 tgatcaacga caacggcacg atcacgccgc tgacggtttc cggtgcggta gacctttccg
     3061 acggtggcac cgtgaccctc aagggcgaca tctccaaact cgcgacggga tcctatccgc
     3121 tcgtgttctg cacctccgcc atcgcgggtc cctggaatgt ccaatacacg ggcacgagtt
     3181 acctcaaata ctttgtgcgg atacgcccgg acgggatcta cctcgacgtg gtgtcaccgg
     3241 gtacgctggt catcttcaga tgattgagga ttcccgcgct tccggctgga acccggaacc
     3301 cgatcacttc tgcacagggg ttccatgccg gagtttcgtt gcgtcggcgt ccatgtccgc
     3361 gccgcccgag gctgtcgagg acgaccagac cacatccttc catgggccct cgtacagttt
     3421 ttcgacacct gtaacaccgt tgaatttaca accggtgatg acaagacgcc ccgatttatg
     3481 cacgatgacc gttgatttgc ggagccccat cagtaagttg ttgtaaaaac cgcaattcac
     3541 aagctgtccg ccatgcggaa ggttgaagcc gatttccccg gtatcggcat agcagccgtc
     3601 gaacagattt ccagaggagg aatcgaagca gatcgatccg gcgagcattt ccggaagccc
     3661 ttttgacagg agagccgctt ccagatccgc attccacctg ctgcgctgct ttttgttggc
     3721 cgcatagaaa tcggaccact cgcgcatgga catccccttc ggcgcgacag tgccgcccca
     3781 gacatggcaa cgcgtgaaac ggttcgagct gcccgtagca cggatgccga ttgtataatc
     3841 aacgacgatg atgtcggtaa aatgacagtc atggacttga acaaggacgc cgatattgcc
     3901 ggcaagcccc ttcatggtgc acttgcagta gacgttgttg gcgatgagtt catagaggtg
     3961 tccgccgttc cagcgcgaga gttcaagtcc ggccttgcgt ccgttgtgga gcgtgatgtt
     4021 ggcgagcgtg aagtgatggg cgttcccgat tcgaagacaa ctcgcgagac ccctgccgtc
     4081 gatatcgccg ccgcggatga agagtccggc attgtcatag atggagccgt cgggattgaa
     4141 gatcgagagc gcatgataat catcatgtcc gtcccaagtg aggacaaact ccatcggtgc
     4201 cgtggcgatg agccgtgccg cgggatgcat ctccagcgaa catcggttcg tgatcacgag
     4261 cggcgtcgca atcgaatagt cgcccttccc gaccgcgaga accccgttcg gcgccgcggc
     4321 gatcgcgcgc cggaaacgtg gcgcgtcatc gtgttcaccg gcaagacgcg ggaaagccga
     4381 cagcaccgca tccggaggaa aggacgcata ccctgcgagc gatatgaggg ccgtaccgat
     4441 cacgattcgt tttttcacgt taagaactcc atggaagggt caggtcaaca acgacccgca
     4501 cttttcttcg attcactcgt atcccacagg ttggttggtc cggtaacaac acaccggttc
     4561 ccatgagcgg cgccccatta tgcgggggag gcgaaaagag tcaatcccgc aatccgcttt
     4621 ccgcccgccc cgaagcgtcc gtagacgggg cactgtggca ccctacgcac ccgcataggg
     4681 gcagcgcacg gaccggtctt ccagaacccg aagcgcgggc ggcgtccggg cgcattcggg
     4741 acgggcgacg ggacagcggt cgtgaaaacg acaccccggc ggaaagttgc ctgccggcgg
     4801 cacctgaccc ggaatggcct tgagccgctt gccgcgcgtg gcggcagagg gcatggcctc
     4861 cagcagggcg atcgaatacg gatgaagcgg ggtacggaag aatgcttcgg acgctgcctg
     4921 ttccacgatt tgcccggcat acatgaccgc gatgcgcgtg gccat
//
LOCUS       DAOSRB010000198         4954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15448_length_4954_cov_7.890794, whole genome shotgun sequence.
ACCESSION   DAOSRB010000198 DAOSRB010000000
VERSION     DAOSRB010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15448_length_4954_cov_7.890794"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1054
                     /locus_tag="PLZ74_09380"
     CDS             221..1054
                     /locus_tag="PLZ74_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98572.1"
                     /translation="MRTLVLGAMVCLLSGFAFTQDLLSELAGPAEAYKTAVEALEKQR
                     LSDMAQAARTYVSVLDGIEKSATAKGEVKIVEAAARERKAALAGRLAAELPAELPALR
                     LQGSRRALLMKQEKVNVDAAGRKKRLDSQYLGVLASLAAKAEPGSTLGKQVAAEREAV
                     LAAGKEGAGGGKASGGEAKASQVPRGKNVVVNGDFEKVDALGKPEGWLFHGPVSVEKE
                     ERNTFVRIKGGTVAGGYYRQEMERPNNVTHGTIRAKLRTHGRIGLDRSRGSANISCKN
                     K"
     gene            1452..2873
                     /locus_tag="PLZ74_09385"
     CDS             1452..2873
                     /locus_tag="PLZ74_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019563642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOR98573.1"
                     /translation="MRALLTVLLLTAGQGCLCPAVKPLPSGLACRGLFHPAPDALFLR
                     DRTGLDTAGDRVVEQQIFDCALALIRDAEQLVVADLFLFQPFDGTGPAPHRPLCEELT
                     AALIRRKREVPSLRAILITDPVNTVYGGRNAPHLCRLRNAGVEVVITDLTRLRDSNPL
                     WSGLWHPLIRPWGNRADAGWMPNPFGAGRVTLRSWLALLNFKANHRKVVIADSGGELR
                     ALVTSANPHDSSSAHTNVGLLFSGPAAHDALACEAAVLAFSGGAAAPEFNSGGKPAVV
                     ESPAWVAVVTEDAVAEAAEELIGGAGRGDMLDLVMFYLADRRIVHALLAAARRGVTVR
                     VLLDPNRDAFGRQKNGMPNRQTARELVRAGVQVRWAETRGEQMHAKLLLRRSVSDTAD
                     LLLGSANFTRRNLRGYNLETDAWLSGSPDAPAFAAATAFVETLWENRGGRRFSTGYDA
                     FRDDSRLRVILARIQEATGLCTW"
     gene            3044..3949
                     /gene="cysK"
                     /locus_tag="PLZ74_09390"
     CDS             3044..3949
                     /gene="cysK"
                     /locus_tag="PLZ74_09390"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOR98574.1"
                     /translation="MAGWTSILGTIGNTPLLKLDRIASFPLYAKAEYLNPAGSVKDRV
                     ALHMIEQAERRGDLRPGMTLAEATSGNTGIGIAFVGVRKGYRVIIVMPDNMSEERRKI
                     IRAFGGELVCTPAAESLNGCLRKLEELRQEIPGLFILGQFVNPDNPEIHYLTTAREIW
                     EQMEGRVDAFVAGVGSGGTLSGVGRFLKERDPAIRVVAVEPAQASALLGQEPGLHRIE
                     GIGDGFIPDVLDVSIIDDVITVTDEQAITTAQQLTRVEGALVGTSAGANVWAAIETGA
                     RIGHDRRIVTILPDRAERYFSTALI"
     gene            4069..4746
                     /locus_tag="PLZ74_09395"
     CDS             4069..4746
                     /locus_tag="PLZ74_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOR98575.1"
                     /translation="MSFFTNLTRGIIRENPTFRLVLGMCPTLAVTTSLENALGMGLAA
                     TFVLVCSNMVISSLRKLIPAAVRIPCYIVIIAAFVTMVDLLMQAYTYALWEKLGIFIP
                     LIVVNCIILGRAEAFASKNGVLNSAADGIGMGIGFTIALSVIATLREFIGNGSVTVWG
                     DLAFTAIHNRAMVLFILPAGGFITLGLILAFLNHLQAVSAQRRGGAAPLPLELDCRHC
                     ALCKPVE"
BASE COUNT          906 a         1448 c         1611 g          989 t
ORIGIN      
        1 cgactgttgc gcccacgcag tggcccgcgg cgacaagtgt aatgatggcg gcgattccga
       61 tcccgatccc gatggcgata gcgatggcgc cgcaatggaa cccagaaacg cattgtcttc
      121 gcacacccct gtcacgaacc gattacccag tccagtcgat cacgtgttga cgcggtcgag
      181 ccgtaagtgt aaagttttcg atatcgaaac ggaagtgcgc atgaggacgt tggttttggg
      241 agcgatggtt tgtctgcttt cagggttcgc gttcacgcag gatcttctgt cggaactggc
      301 tgggccagcg gaagcctaca agacggcggt ggaagcgctg gaaaagcagc ggctgtcgga
      361 tatggcgcag gcggcgagga cttatgtgtc ggtgctggac gggatcgaga agtcggccac
      421 ggccaagggg gaggtcaaga ttgtcgaggc cgccgcgagg gaacgaaagg cggcactggc
      481 cgggagactg gcggcggagt tgcccgccga acttccggcc ctgcgtcttc aaggtagccg
      541 cagggcattg ctgatgaaac aggaaaaggt gaacgtcgat gcggccggac gcaaaaaacg
      601 gctcgacagc cagtatctgg gcgtgcttgc ctctctcgcg gccaaggccg aacccgggtc
      661 cacgctcggc aagcaggttg cggctgagcg ggaggcggtg ctggcggctg gcaaagaggg
      721 tgctggcggc ggcaaggcca gcggtggtga agcgaaagcg tcccaggttc cgcgcggcaa
      781 gaacgtggtg gtgaacgggg atttcgagaa ggtcgatgcg ctcggcaagc cggagggttg
      841 gctttttcat ggacccgttt cggtcgagaa ggaggagcgg aacaccttcg tcaggattaa
      901 gggagggacg gtagcaggag ggtattatcg tcaagaaatg gaacgcccca ataatgtaac
      961 ccatggtacg atccgtgcga aattgagaac gcatgggcgc attggtttag atcgatccag
     1021 agggagtgcg aatatctcat gtaaaaataa gtaatctttt ggatcatagc tatatcgggt
     1081 cggaaagacg gcgtatggac aacgattcac ggggaaggtg caattcctaa agaggcgact
     1141 tggttttgtt ttgaactcag ttataaagga agcaagggcc aggtcgactt cgacgacgtg
     1201 gaagtgacgt tcaagtaggc cggagcgcaa tctcggcttc ggaaggaacg cgcaacgccc
     1261 aagtaaggga agagcgcgta caaagaggcg ggatcgactt cgactttctg cggtttgctc
     1321 gggttgtggg ctccgtccgt gttagacaca aacgaacgca ttttccgtca aatgtaaaat
     1381 tgccggaaga ctggcgcttc ccgtcgtggg catgttatgc tgtctccatc atgccggaac
     1441 gttcctgttt tgtgcgcgct ctgttgacgg ttcttctgct gactgccggt caaggctgtc
     1501 tgtgccctgc cgtgaaaccc ctgccgtcgg ggttggcttg ccgcggcctg ttccaccccg
     1561 cgccggacgc gctctttctt cgtgaccgca ccggacttga caccgcaggt gaccgggtgg
     1621 tcgaacagca gattttcgac tgcgctttag ccctgatccg cgacgcggaa cagctcgtgg
     1681 tggcggacct gttccttttc cagccgtttg acggaaccgg tccggcgcct caccgcccac
     1741 tatgcgaaga actgaccgca gccctgatcc gccgcaagcg cgaggtccct tccctgcggg
     1801 cgattctcat caccgatccg gtcaacaccg tctacggcgg tcgaaacgcc ccgcacctgt
     1861 gccgccttcg caatgccggg gtcgaagtgg tgataaccga tctcacccga ttgcgcgaca
     1921 gcaacccatt gtggtcgggg ctctggcatc cgctgatccg gccgtgggga aaccgggccg
     1981 atgcgggatg gatgcccaat ccgttcggcg cgggacgggt caccctgcgg agctggctgg
     2041 ccctgctcaa cttcaaggcc aaccaccgga aggtggtgat cgccgactcc ggcggcgagt
     2101 tgcgtgcgct ggtcacctca gccaacccgc atgacagcag cagcgcccat accaacgtcg
     2161 ggctgctttt cagcggtccc gcggcccacg acgcgctcgc gtgcgaggcg gcggttctgg
     2221 cgttcagcgg cggcgcggcg gcacctgaat tcaacagcgg cggcaaaccg gcggtcgtcg
     2281 aatccccggc atgggtcgcc gtagtgaccg aagacgcggt cgccgaggcc gccgaggagc
     2341 tgatcggcgg cgcggggcgt ggggacatgc tggatctggt gatgttttat ctcgccgacc
     2401 gccgtatcgt ccacgcgctc cttgcggcgg cgcggcgcgg tgtcacggtc cgcgtgttgc
     2461 tggatcccaa ccgcgatgcg ttcggacgtc agaagaacgg catgcccaac cgtcagaccg
     2521 cgcgcgagtt ggtgcgagcc ggcgtgcagg tgcgctgggc cgagacccgc ggcgaacaga
     2581 tgcacgccaa attattgctg cggcggagtg tcagcgacac ggccgatctg ctgctcggtt
     2641 ccgccaattt tacccgccgc aatctgcgcg ggtacaatct ggagaccgat gcgtggctct
     2701 ctggctcgcc ggacgccccc gcgttcgccg cggccaccgc ttttgtggaa accctgtggg
     2761 agaaccgtgg cggtcggcgt ttctccaccg gttacgacgc cttccgcgat gactcccggc
     2821 tgcgggtgat tctcgcgcgc attcaggagg ccaccggcct gtgcacatgg tgaccgaaac
     2881 agcgggaaac acgcgctcca cacgcacgcc ccgtgcggcc aaccggttac gccggtctcc
     2941 cgccggggcg gttgcgtcgc catcggacgt gtgatagagt ttcgcaatca actggcccgc
     3001 ccggcaggga acgcgcgggc agagcaggag gagtgttcgt atcatggcag gctggacaag
     3061 cattttggga accatcggca acacaccgtt actgaagctc gaccggatcg cgtctttccc
     3121 cctctatgcg aaagccgagt acctcaatcc ggcgggcagc gttaaagacc gggtggcgct
     3181 gcacatgatc gagcaggcgg agcggcgcgg cgacctgcgt cccggcatga cccttgccga
     3241 ggccacctct gggaatactg ggatcggcat cgcctttgtg ggggtgcgta agggatatcg
     3301 ggtgatcatt gtgatgcccg acaacatgag cgaagagcgg cgcaagatca tccgggcctt
     3361 tggcggcgaa ctggtctgca ctccggcggc ggagagcctc aacggctgtc tgcgcaagct
     3421 cgaggaactg cggcaagaga tccccggtct ctttatcctt ggccagttcg tgaaccccga
     3481 caaccccgag atccactatc tcaccacggc gcgggaaatc tgggagcaga tggaggggcg
     3541 ggtcgacgcg tttgtcgcgg gtgtcggaag cggcggcacg ttgtccggcg tgggccggtt
     3601 tctcaaagag cgcgacccgg cgatccgtgt ggtcgcggtg gagcccgccc aggcttccgc
     3661 cctgctcgga caggaacccg gcctgcaccg gatcgaaggg atcggcgacg ggttcatccc
     3721 cgacgttctg gacgtctcga ttattgatga cgtcatcacg gtcactgacg agcaggcaat
     3781 caccaccgcg cagcagctga cacgcgtgga aggggcgctg gtcggtactt cagccggggc
     3841 gaatgtctgg gccgcaatcg aaaccggggc gcgcatcggg catgaccgaa ggatcgtcac
     3901 gatcctgcct gaccgcgccg agcgttattt cagcaccgca ctgatctgag gtcggatcgg
     3961 gtcagtgcgg ggagttgtat ccgctgaaca ttctggttcg gcgggccttg cttttttcgc
     4021 cgtctttccg gatactatgg attttccgtt tgcaacctct ggggaatcat gagctttttc
     4081 actaacctga cccgcggcat catcagagag aatccgacct tccggctggt gctcgggatg
     4141 tgtcccacgc tggccgtgac cacgtcgctg gaaaatgccc tcggtatggg tttggccgcc
     4201 acgttcgtgc tggtctgctc caatatggtg atctcctcgc tgcgcaaact cattcccgcc
     4261 gcagtgcgca tcccctgcta cattgtgatc atcgcggcgt tcgtcaccat ggtggacttg
     4321 ctgatgcagg cttacaccta cgccctgtgg gagaagctcg ggattttcat tccgctcatc
     4381 gtggtgaact gcatcattct tggacgggcc gaagccttcg catccaagaa cggcgtactg
     4441 aatagcgcgg cggatggaat cggcatgggg atcgggttca cgatcgcgct ctccgtgatc
     4501 gccacgctgc gtgaattcat cgggaacggg agcgtcaccg tctggggtga cctcgccttc
     4561 accgcgatcc acaaccgtgc gatggtgctt ttcattctgc ctgcgggcgg gtttattacc
     4621 ctcggcctga ttctggcctt cctcaaccac ctgcaggctg tctccgcgca gcgccgcggc
     4681 ggtgccgccc cgctgcctct cgaactcgac tgccgccact gcgccctgtg caagcctgtc
     4741 gagtgatcgg ctcggctcaa agttgcgatc cggtttttaa cttaaaaaga gcagttgaag
     4801 gctcaactct ctttggtttc aaggtttcag ggtttcatgg tttcagggtt tctggtttca
     4861 gggtttcatg gtttctggtt tcatccaaat cggggtcggt atcgctatcg gtatcggtat
     4921 cgtcgacccg cgacccgcga cattcgaccc gcga
//
LOCUS       DAOSRB010000199         4944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15480_length_4944_cov_6.397832, whole genome shotgun sequence.
ACCESSION   DAOSRB010000199 DAOSRB010000000
VERSION     DAOSRB010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15480_length_4944_cov_6.397832"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLZ74_09400"
     CDS             complement(<1..324)
                     /locus_tag="PLZ74_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98576.1"
                     /translation="MTRRLTSGCGRVQVALAALVICGGAAAAAAAGDRVPVFPSPEWD
                     AGKVVQRGLSVTREGDALVFSQGRREAWPQALLPVRPGMAGCSGLVVRAENTGKDLLK
                     IRFAAP"
     gene            complement(411..2753)
                     /locus_tag="PLZ74_09405"
     CDS             complement(411..2753)
                     /locus_tag="PLZ74_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOR98577.1"
                     /translation="MSVISGIRLEKKVWSGLAFCVRGWCAGLAMGLLGIQAAAAGTVV
                     SRVTPGGGMPSLTWESYPLGVYRVWRSENAAGPYDTQVGGDVTATGYTTTLTDPQSGA
                     AGSRRYYRIETVSPGYANYLILDVSGGTNAASWPVVATNAVPDLLTDPAYRTTKVVLK
                     LIGAGSFKMFGLYDVTLTRPFYIGVFELTQDQCVNMTGSAYASQDHGATRPANNFSFA
                     DLRGGTNDAAGWGWPADNLVAPNSLIGRLRAKTGSDNFDLPTSAQWEYASRAGTTTDF
                     YNGTDANPSDGTDFSMVTNIARCKANNDIPIDGQTGHAPVGSYLPNAWGIYDTHGNVA
                     ELLLDREWTGAEYAGTDPLGPTNVNKRVFRGGQSKSPNTSYLKNTLVNGADPEKVYVP
                     GVMGSRLVLRLPEDETLGQAGAEGGENAVITRVDPVAVPMITWVSLPDAVYGVFRSES
                     PTGPFETQIGENLTGTGGLLSVMDDSVLNNAYHYRVEQLASGYARYMIVDVSAGSGAT
                     SWPVSYTNVLPELLTDNIYKTDRIVLKLLPPDTYTMRQPVNTGVPVTLTRPFYAGVFE
                     VTRAQYTRVTGISKGTTAHPVGDVSYTSLRGGTADGIDWPTTANSVAAGSFMGLLRAK
                     TASGGKTLLFDLPTDAQWEYACRAGTSSHFNNGRNAASKDDWAAINDIAWHKNNSGGG
                     AQLVGLLNPNGFGLYDMHGNITEFCLDWSWNGPTAHAVDPTGPTTAADGKRIYRGGTY
                     SSPNLSTLYSSTRGGFYHTSTQGNIGFRLYCQLPEEAGQP"
     gene            complement(2767..4221)
                     /locus_tag="PLZ74_09410"
     CDS             complement(2767..4221)
                     /locus_tag="PLZ74_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98578.1"
                     /translation="MGVTNQARGWVRVRGLCRAMAGCGVLVCATGFSVRAGVVPNAGA
                     EAAVACVSAAPADGLFSAVFATNVAEVTFGAVGTDRALSLSCVSIDGTVASGASPTGM
                     AAFSQGTFDAARTGVSVRLGRVSGELHGTGPDQAFGALAWSGAGGLTNPATLWAGLID
                     GVVSANAGPDGTLAAALCAGTAVDVTLGSGAVLFGGTYGWDTGDGSAASAASALQTLL
                     AKVKARTATAAESNALVTAAADGFAVLGGHGEDTLAFASGNTAVFGTVALGEGYDRVA
                     LAGVASGERVSLPTMDGVELLAVTNCPDVSVANTAGLADLFVGSDSLCRLKDASCVFD
                     GLYGTGTVVPEGTLQATGMIAVARFEPAVTGAVLSVNGNLTLGNGVTCALRTAEEEGA
                     WVNDRVAVSGALTVAGGGTIDLGCAEEAPLALNTRLVLFTAADGIANPGALSSWQLIG
                     TGRDDERKVIRTIRAEGNAVVAVIAVGGTFISLR"
BASE COUNT          901 a         1783 c         1383 g          877 t
ORIGIN      
        1 cggcgcggca aagcggattt tgagaaggtc tttgccggtg ttctcagccc ggaccaccag
       61 ccctgaacac cccgccatcc ccggccgcac gggcaacagc gcctggggcc aggcctcccg
      121 ccgcccctga gagaacacca gggcgtcccc ctcgcgcgtg acgctgagcc cgcgctgcac
      181 aaccttcccg gcatcccact ccggcgacgg gaaaacggga acccgatccc ccgccgcggc
      241 ggccgcagcc gccccgccgc aaataaccag cgccgcaagc gcaacctgca cgcgcccgca
      301 tcccgatgtc aatcgacgtg tcataaaacc tctctcccgg cgcggccttg caactcccct
      361 gatccacctc gtcacaagtc cgcgcgtcat tcacttgtcc gagccggttt tcatggctgt
      421 cccgcctctt ccggcagttg acagtagagc cgaaagccga tattcccttg tgtgctggtg
      481 tgatagaagc cgccgcgggt tgaagagtag agcgtggaca ggttcggact gctgtaggtg
      541 ccgccgcggt aaatgcgttt gccgtccgcg gccgtcgtgg ggcctgtcgg gtcgaccgcg
      601 tgagcggtcg gtccgttcca gctccaatcc agacagaact cggtgatgtt cccgtgcatg
      661 tcgtagagtc cgaagccgtt gggattgagc aatcccacga gctgtgctcc gcctcccgaa
      721 ttatttttgt gccaggcgat gtcgttgatc gcggcccaat cgtctttcga ggccgcgttc
      781 cgcccgttgt tgaagtgcga actcgtgccc gcgcggcagg cgtactccca ctgcgcgtcc
      841 gtgggcagat cgaacagcag ggttttccct ccgctcgcgg ttttggcccg gagcagcccc
      901 atgaaagaac ccgcagccac gctgttcgcg gtcgtgggcc agtcaatgcc gtccgccgta
      961 ccgcctcgga ggctggtgta ggagacatcc ccgaccggat gggcggtcgt ccccttgctg
     1021 atgcccgtca cccgggtgta ctgcgcgcgg gtcacctcga acacgcccgc gtaaaacgga
     1081 cgtgtcaacg tgaccggaac ccccgtgttc accggctgcc gcatggtata ggtgtccggg
     1141 ggcagaagtt tcagcacgat ccgatccgtt ttgtagatgt tgtcggtcag cagttccggc
     1201 agcacattgg tgtaactcac cggccaggag gtcgccccgg accccgcgct cacgtccaca
     1261 atcatgtagc gcgcgtatcc cgacgccagc tgctcgacgc ggtagtggta ggcgttgttc
     1321 aaaacactgt catccatcac cgagagcaag ccgcccgtac ccgtcagatt ctcgccgatc
     1381 tgcgtttcaa agggtccggt tggcgactcg gagcggaaca cgccgtacac ggcgtccggc
     1441 aggctgaccc aagtaatcat cggcaccgcc acagggtcaa cccgcgtgat cacggcgttt
     1501 tctccccctt ccgcccctgc ctgcccgagg gtctcatcct ccggcagccg caacaccagc
     1561 cgcgacccca tcacgcccgg cacatacacc ttttccggat ccgcgccgtt caccaacgtg
     1621 tttttcaagt aactcgtatt gggcgacttg gactggccgc cccgaaacac gcgtttgttg
     1681 acattggtcg gccccagcgg atccgttccc gcgtattccg cccccgtcca ttccctgtcc
     1741 agcagcagtt ccgccacatt gccgtgcgtg tcataaatcc cccaggcgtt gggcaggtag
     1801 gagccgaccg gcgcatgccc cgtctgcccg tcgatcggga tgtcgttgtt cgccttgcag
     1861 cgcgcgatgt tggtcaccat agagaagtcc gtgccatccg aaggattcgc gtccgtgccg
     1921 ttatagaaat ccgtcgtcgt tccggcacgg ctggcgtact cccactgcgc gctggtcggc
     1981 aggtcgaagt tgtcgctgcc ggttttggcc cgcagacggc cgatcagcga attcggggca
     2041 accagattgt cggcgggcca accccatccc gccgcatcgt tcgtgccgcc gcgcaaatcg
     2101 gcgaatgaga aattgttcgc cggacgggtg gcgccgtgat cctggctcgc ataagccgaa
     2161 ccggtcatgt tgacgcactg atcttgagtc agctcaaaca cgccgatata gaagggccgg
     2221 gtcagggtca catcgtataa gccgaacatc ttgaaactgc ccgcgccgat cagtttcagc
     2281 accaccttcg ttgtccggta cgcgggatcg gtcagcaggt ccggcacggc attggtcgca
     2341 accaccggcc atgatgccgc attcgtgccg ccggacacat cgaggatcag gtagttcgca
     2401 tacccgggcg acaccgtctc gatccggtag taccggcggc tgcccgcggc cccggattgc
     2461 ggatccgtca gtgtcgtcgt gtaccccgtt gccgtcacgt cgcccccgac ctgcgtgtcg
     2521 tacggtccgg ccgcattttc cgaccgccag acgcggtaca cgcccagcgg gtacgactcc
     2581 caggtcaggg agggcatgcc accgcccggc gtgacgcgtg aaacgactgt ccccgccgca
     2641 gccgcctgaa ttccgagcaa ccccatcgcc aaccccgcgc accagccccg aacgcaaaag
     2701 gccagcccgc tccacacttt cttttccaac cggattcctg aaatcacgct catgtctgcc
     2761 tcctcctcaa cgcaacgaaa tgaatgtgcc gcccaccgcg atgactgcca cgaccgcgtt
     2821 accctccgca cggatggtcc ggatcacctt ccgctcgtca tcgcgtccag tgccaatcag
     2881 ttgccagctc gacagcgcac cgggattcgc gatcccgtcc gcagccgtga acaggaccag
     2941 gcgcgtgttc aacgcgaggg gtgcctcctc agcgcatccc agatcgattg tgccgccgcc
     3001 cgccacagtc aacgccccgc tgacggccac ccggtcgttg acccatgcgc cctcctcctc
     3061 tgccgtgcgc aaggcgcagg tcaccccgtt gcccagggtc agattcccat tgacgctcag
     3121 caccgcgccg gtcaccgcag gttcgaagcg cgcgaccgcg atcatcccgg tcgcctgcag
     3181 cgtcccttcc ggcacgaccg tgcccgtccc gtaaagcccg tcaaacacgc aggaagcatc
     3241 cttcagccga cacagggagt cgctccccac aaacagatcc gcaagccccg ccgtattcgc
     3301 gaccgacaca tccgggcagt ttgtgaccgc cagcagttcc accccgtcca tcgttggcag
     3361 gctcaccctc tctcccgacg ccacacccgc caaggccacc cggtcatacc cctcgcccag
     3421 ggcgaccgta ccgaaaaccg ccgtgtttcc gctcgcgaac gccagcgtgt cctccccgtg
     3481 tccgccgagc acggcgaacc cgtctgcagc cgccgtaacc aaggcgttgc tttcggccgc
     3541 agtggccgtc cgcgccttaa ccttcgccag cagggtctgc agggccgacg ccgcgctggc
     3601 ggccgaaccg tcccccgtgt cccatccata ggttccgccg aagagcaccg caccggagcc
     3661 cagcgtcacg tccaccgccg tgccggcgca gagcgccgcc gccagcgttc cgtccgggcc
     3721 ggcattcgcg ctcaccaccc cgtcgatcaa ccccgcccac agggtggccg ggttcgtcaa
     3781 tccacccgct cccgaccacg ccagcgcacc gaacgcctga tccgggccgg tgccgtggag
     3841 ttcgccgctc acgcgtccga gacgcaccga caccccggtt ctggccgcat caaaggtccc
     3901 ctgcgaaaaa gcagccatgc cggtcggtga ggccccgctt gccaccgtcc cgtcgatcga
     3961 gacgcacgac agcgacaacg cccgatccgt cccgaccgcg ccgaacgtca cctcagccac
     4021 attcgtcgcg aagaccgctg agaacagccc gtcggcaggt gccgcgctca cgcaggccac
     4081 tgcggcctcc gcacccgcat tcggaacgac tcccgcccgg acggaaaaac ccgtggcgca
     4141 caccaagacg ccacaccccg ccatcgcacg gcacagcccg cgcacgcgca cccatcccct
     4201 tgcctgattg gtcacaccca taccactcct ccttccgcct gtcggccaaa ccttgtctcg
     4261 ttgatttata ttataggaaa aaaaggcgtg ttccgacaat ctcgataatg cgaactcgac
     4321 cctcgatttt ccgaactaac gaaaccccct cagaacacct cgggacggaa acagggatgt
     4381 gcgaaaccca tgcgtttctg gtgaaaggga gagcagagag tcggaagcgc gggcacctct
     4441 ggtggacatt gttgactttg ttgacgttgt gtggagacct ggacggcgga agcggtgagg
     4501 gtaattttgt ctcacactgc aaaacagcgc cattgtggct cacttggttc tgcggatatg
     4561 tggagccatg aaacacctcc actttttgtg accactcagt ggtcacaaag atgaggttaa
     4621 aaccttgcac catgcagccg cgccccgccc gcggtcattt acagcacttc gggcgctttt
     4681 gggggctgtc tccgcgcgcg ccctatcgca tgccccgcgc gcgcccggcg cccgccctcc
     4741 cacctgtttt tacactcatc attgcgacca cttggtggtc gcaataaaaa tctgtggatg
     4801 aaaaaaaacc gagccatgcc ttttcgtgtt gtggcacggt gggtgcttat atcagggtac
     4861 gacaaggaga ccacaccatg accgatttga acaaatggac ccaaaaagcg caggaggcgc
     4921 tgcagaacgc gcgccaactc gcca
//
LOCUS       DAOSRB010000200         4926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15559_length_4926_cov_6.121741, whole genome shotgun sequence.
ACCESSION   DAOSRB010000200 DAOSRB010000000
VERSION     DAOSRB010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15559_length_4926_cov_6.121741"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..839)
                     /locus_tag="PLZ74_09415"
     CDS             complement(105..839)
                     /locus_tag="PLZ74_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR98579.1"
                     /translation="MRIVQIWMSAVMAVSALWGAGVCCGGATVPNTLTEQERREGWRL
                     LWDGRSGDGWRSIRNDNFPTQGWEIADGVLTVLPKNAGGGAGDIITRETFASFILKVD
                     FRLTEAANSGIKYLFHPKRNGGTTLEYQVLDAKHPDATKGRDGNRTVASLYDVMPAAG
                     AKPKPVGEWNTAMIVCRGRQVEHWLNGEKVLAFERGSEAFRQAVGLSKFNKYPQWGEQ
                     VAGHILLQDHQDRVSYRNIKIRVLDQ"
     gene            complement(862..1638)
                     /locus_tag="PLZ74_09420"
     CDS             complement(862..1638)
                     /locus_tag="PLZ74_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR98580.1"
                     /translation="MELCVLASGSGGNALYVKGGGVALLVDAGLSSRQLVARMRAADI
                     APESIHAILLTHDHSDHYKGVDVFSRHYPVRLFANEGTASGVEREVRSLGREWEIFET
                     ATSFEVGGLTVEAFSVSHDASDPVGFVLDDGTVRLCVITDLGQATPLVRVKLATCDVA
                     VVESNHDYDMLMQSQRAWTLKTRIAGRSGHLSNDDAADLVRSALSERLRALFLAHISG
                     QCNTPALAFATMADMLKREGRDDVRLEVTSQDQVSTRYVF"
     gene            complement(1649..2638)
                     /locus_tag="PLZ74_09425"
     CDS             complement(1649..2638)
                     /locus_tag="PLZ74_09425"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOR98581.1"
                     /translation="MANLKTSYLGLELANPLIVSSNPLTDRADTVADLERAGASAVVM
                     RSIFEEQIRADVSEMVKALAGDTSMAAMEYLRADLPGQLGPEAYLENLAAIRKRVRIP
                     VIASVNCVAADKWVAYARKIEQAGADALELNLYHMPIDPEETGKEVEARRLALVQAVL
                     AEVRLPVAVKLSHHYTSLLPFARRLDALGVKGLVLFNRFLQTDVDIENESIFFTPNYS
                     TPYVLHTQLRWTAVVRDWVRCALAISGGIHSGAELVKALLVGADVGCICSVLHVRKDF
                     GVIREILEDLAAWMDRKGYASLADFRGALRETGRKNGQGFERQQYVETASRVS"
     gene            complement(2646..3887)
                     /gene="argG"
                     /locus_tag="PLZ74_09430"
     CDS             complement(2646..3887)
                     /gene="argG"
                     /locus_tag="PLZ74_09430"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOR98582.1"
                     /translation="MALTLTDLRGKTVGACVSGGLDSRTIAKVMVEAGVKVVAFSADL
                     GQPDEKNINDIRKRMAPCGVDTVMVDLKEAMAEACFQVIEAQAMYDGGYWNSTGIARA
                     VTVRGLLPEMRKRGCSVLVHGATGRGNDQMRFERYTNVLAPSMKVYSPWRDAELLKRF
                     PGRTQMVDFLAQFGIQAFVGAKKKYSTDANLAGLSHEAEDLESMETSMLIVEPTMGVW
                     PQQAPDKMEKVAVRFVKGRAVQINGKDVTPLEAMRTANRLAGRNGVGMKHALENRIIG
                     TKSRGVYEAPGMELLGTCLRYVYQATMDRRAGLLFGQLSKLVADQIYDGRYFDPVTQA
                     ARAAIATFARHASGTVEVGLYKGNLFFNSLTGCKASVYNEADSSMEASDGLNPVSSQG
                     FAEIQSVEARMLARAGQIVAK"
     gene            4364..>4926
                     /locus_tag="PLZ74_09435"
     CDS             4364..>4926
                     /locus_tag="PLZ74_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98583.1"
                     /translation="MKKLLVIIAVCACVRATLAEPVFWEAASPVTLDATKTFELRVGK
                     APQTGDVSLLVALSAPVDDTFRPTVTLNGVTATKETHAPLATWLPGFDATPIRSPRET
                     REIQAGYRYAFPLSALKAGGNTVALGPAVDTPKVHALALDVDPTSEIVVENDDFRLVV
                     GKDARVKSLVVKKTNEECADAGMQLPL"
BASE COUNT         1008 a         1706 c         1306 g          906 t
ORIGIN      
        1 acagcctttg agattgcggg ctccgcccgc atctcaatac gtcaggacgg accggaaagc
       61 ggtgccgccc gttggcgggg cggtttcccc gaaacggcta caggctactg atcaagcacc
      121 cgaatcttga tgttgcggta cgagactcgg tcctgatggt cctgcagcag gatatgaccc
      181 gcaacctgct ccccccactg cggatatttg ttaaatttgc tgagcccaac cgcctgccgg
      241 aacgcctcgg agccgcgctc gaacgcaagc actttctccc cgttgagcca atgctcgacc
      301 tgccgccccc ggcatacgat catcgccgtg ttccactcac cgaccggttt cggcttagct
      361 cctgccgccg gcatcacatc gtagagcgag gcaaccgtgc ggttgccgtc gcggcctttc
      421 gtggcgtcgg gatgcttggc gtccagcacc tgatattcca gcgtggtgcc gccgttgcgt
      481 ttgggatgga agagatactt gatcccgctg ttggccgctt ccgtgagccg gaagtccact
      541 ttgaggataa atgaggcgaa ggtctcgcgc gtgatgatgt cccccgcgcc cccgcccgcg
      601 tttttaggca atacggtcaa gaccccgtcc gcgatctccc aaccctgggt ggggaagttg
      661 tcgttccgga tgctacgcca gccgtccccg ctccggccgt cccacagcaa acgccaccct
      721 tcacgccgtt cctgctcggt cagggtgttc ggcaccgtgg ccccgccaca acagaccccc
      781 gcaccccata gtgccgacac cgccatcacc gcactcatcc atatttgaac gatccgcatg
      841 cctctaccct cctttgtctg tttaaaaaac ataacgtgtg ctgacctgat cctgactggt
      901 tacctccagc cgcacatcat cccgcccctc ccgtttcaac atatcggcca tggtggcgaa
      961 agccaaggcc ggcgtattgc actggccgct gatatgcgcg agaaacaacg cgcgcaaccg
     1021 ctccgacaat gcggaccgaa ccaagtccgc cgcatcgtca ttcgacaagt gtccgctgcg
     1081 gcccgcaatc cgcgttttga gcgtccaggc ccgttgcgac tgcatgagca tatcgtagtc
     1141 gtgattgctc tcgaccaccg cgacatcgca tgtcgcgagt ttgacacgca ccagcggagt
     1201 cgcctgcccg agatccgtga tcacgcaaag ccgcaccgtc ccgtcatcca gcacgaaccc
     1261 caccggatct gatgcgtcat gcgaaaccga aaacgcttct accgtcaatc cccccacctc
     1321 aaaactcgtc gcggtctcga agatttccca ctcccggccc agcgagcgca cctcccgttc
     1381 aacccccgaa gcggtcccct cgttcgcaaa gaggcgcacc ggatagtgac ggctgaacac
     1441 gtcaaccccc ttgtaatgat cggaatggtc atgcgtcagc agaatcgcat ggatcgattc
     1501 gggggcgata tccgctgccc gcatgcgcgc gaccagttgg cgtgacgaca gccccgcatc
     1561 caccagcaac gcgaccccac cccccttcac ataaagggcg ttgccaccac ttccgctcgc
     1621 taacacacac agttccatct catccgcctt acgaaacccg cgaggccgtc tcaacgtact
     1681 gctgccgttc aaagccctgc ccgttcttgc gaccggtctc acgcaacgca ccccggaagt
     1741 cagccagcga agcatatccc ttacggtcca tccaggcggc gagatcctcc aggatttccc
     1801 ggatcacgcc gaaatccttg cgcacgtgca gcaccgaaca gatgcacccc acatccgccc
     1861 cgaccagcaa cgccttgacc agttcggctc ccgagtgaat cccgccgctg attgccagag
     1921 cgcatcgcac ccagtcacgc accacggcgg tccagcgcaa ttgcgtgtgc aacacgtagg
     1981 gcgtcgaata gttcggcgta aagaagatcg actcgttctc gatatccaca tcggtctgca
     2041 aaaaccggtt gaagagcaca agccccttca cccccagcgc gtccaaccgg cgtgcgaaag
     2101 gcagcagcga ggtgtagtgg tgactgagtt tcaccgccac cggcagccgg acctccgcca
     2161 gcaccgcctg gaccagcgcg agtctgcgcg cctcaacctc cttgcccgtc tcctccggat
     2221 cgatcggcat atgatagagg ttgagttcca gcgcatcggc cccggcctgc tcgattttgc
     2281 gcgcatacgc gacccacttg tccgctgcca cgcagttgac gctggcgatc accggaatcc
     2341 ggacacgctt gcggattgcc gccagatttt ccagataggc ctccggacca agctgccccg
     2401 gaagatcggc gcgcagatac tccatcgccg ccatgctggt atcccccgcc agcgccttaa
     2461 ccatctcgga cacgtccgcc cggatctgct cctcgaaaat cgagcgcatc accaccgccg
     2521 acgcaccggc gcgttccaga tcggctaccg tatccgcccg gtctgtaagg gggtttgaac
     2581 tgacaatcag cggattcgcc aactcaagtc ccagataaga tgttttcaga tttgccatgg
     2641 acccctcact tggccacgat ctgcccggca cgcgccagca tgcgggcctc gacactctgg
     2701 atctccgcaa acccctgcga actgacgggg ttgagcccgt cactggcctc catggaggag
     2761 tccgcctcat tgtaaaccga agccttgcag ccggtaagcg agttgaaaaa caggttgcct
     2821 ttgtagaggc cgacctcaac cgtgccggaa gcgtgccggg caaaggtggc gattgccgcc
     2881 cgggccgcct gcgtcaccgg atcgaaatac cttccgtcgt aaatctggtc ggccaccagt
     2941 ttggagagtt gcccgaacag caatcccgcg cggcgatcca tcgtggcctg atacacgtag
     3001 cgcaggcagg tgcccaacaa ctccataccc ggcgcctcgt acacgccgcg gctcttggtc
     3061 ccgatgatac ggttctccag cgcgtgcttc atgccgacgc cgttccggcc tgcgagccgg
     3121 ttcgccgtcc gcatcgcctc gagcggcgtc acatccttgc cgttgatctg aaccgcacgg
     3181 cccttcacaa accgcacggc caccttctcc atcttgtccg gcgcctgctg cggccacacc
     3241 cccatggtcg gctcgacgat cagcatcgaa gtctccatgc tctccaaatc ctcggcctcg
     3301 tgggagagcc ccgccagatt ggcgtcggtc gaatatttct ttttcgcgcc gacaaaagcc
     3361 tgaataccga attgcgcaag aaaatcgacc atctgcgtgc ggccgggaaa acgcttgagc
     3421 agttccgcgt cacgccacgg cgagtagacc ttcatcgaag gcgccaacac gttggtgtac
     3481 cgctcaaacc gcatctgatc attcccacgc ccggtcgcgc catgcaccag cacggaacaa
     3541 ccgcgcttcc gcatctcggg aagcaacccg cggaccgtca ccgcgcgcgc aatgccggtg
     3601 gaattccaat aaccgccgtc atacatcgcc tgggcctcaa tgacttggaa gcaggcctca
     3661 gccatggctt ccttgagatc gaccatcacc gtatccacac cgcaaggcgc catccgtttg
     3721 cggatgtcgt tgatgttttt ctcgtccggc tggccaagat ccgcagagaa ggcgacaacc
     3781 ttcacccccg cctcgaccat caccttggcg atcgtccggc tgtccaagcc gcccgaaacg
     3841 cacgcgccta ccgttttgcc ccgtaaatcc gtcaatgtca gtgccatacc gttctctccc
     3901 gtcagatcga ttccgatggc ataagcgtac cgagtagtcc ggccaaaatc aacacctata
     3961 actcaaaagc cgcgccgcag ccgacaggac ccgttcctcg aacaaactac gattcagcgt
     4021 tccagtcact ctcgggcgtc cgagacgtcc gcggacggtt cgcgaaaccg tccgctgccc
     4081 ctcttgagca ccgccgcaca ggttcacagc ggagcggcac acgtacacgt ttacaaaaca
     4141 cgggaagagg agtcatcgtg cacaggcaca tgaacgagta tggagagggc aaagctggta
     4201 aagcgtagag agcggtgttt gggggttgac ctcccccccc ccagaatcgc acaatgatga
     4261 acaccggagg ttgaccccgg tacgaagtgt gtcaccggtg tcaagtcaaa gcgcgatacc
     4321 ggagaactcc atgcccgaaa agtgattccc gaaggagaag caaatgaaaa aacttctggt
     4381 gattattgca gtgtgcgcct gcgtgcgtgc caccttggcc gaacctgttt tttgggaagc
     4441 cgcgtcgccg gtgacgctcg acgcgaccaa gaccttcgag ctgcgcgtcg gcaaggcacc
     4501 gcagacgggc gacgtgtcgc tcttggtcgc gctcagtgcg ccggtggatg acacgttccg
     4561 tccgacggtg acgctcaacg gcgtgacggc caccaaagaa acacacgcac cgttagcgac
     4621 ctggctaccc ggtttcgacg caacgcctat tcgctcccca cgggaaaccc gtgaaatcca
     4681 ggctggctac cggtacgcct ttcccctgtc agcactgaag gcgggcggaa acaccgtcgc
     4741 gctcggcccc gccgtcgaca cgcccaaagt tcacgcgctc gcgcttgatg tagacccgac
     4801 tagcgagatc gtcgtggaaa acgacgattt ccgcctcgtc gtggggaaag atgcgcgcgt
     4861 aaaatccctt gtcgtgaaaa agacgaacga agagtgcgca gacgcaggca tgcagttgcc
     4921 gctttt
//
LOCUS       DAOSRB010000201         4923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15570_length_4923_cov_7.659408, whole genome shotgun sequence.
ACCESSION   DAOSRB010000201 DAOSRB010000000
VERSION     DAOSRB010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15570_length_4923_cov_7.659408"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..292
                     /locus_tag="PLZ74_09440"
     CDS             47..292
                     /locus_tag="PLZ74_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98584.1"
                     /translation="MATKKAAPAKKKAAPAKKAAPAKKAAPKKAAVKKAPAKKAAPKK
                     AVVKKAAPKKAAVKKAPAKKAAPKKAVKKVAAKKAKK"
     gene            477..1781
                     /locus_tag="PLZ74_09445"
     CDS             477..1781
                     /locus_tag="PLZ74_09445"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOR98585.1"
                     /translation="MKQIGVGLLGFGTVGAGVADGLQRNRKVMARRLGADVVLKRIAD
                     LDTVTDRGVAIDPAILTPDALSVIHDPEIRIVVELIGGTGVAKELVLAALNAGKPVVT
                     ANKKLLAEHGEEIFSAAERKGVDIYFGASVGGGIPIIRVLREGLAGNEIQSIHGILNG
                     TCNYILTRMEQEGLPFDAVLRDAQQKGYAEADPSLDIDGFDTAHKAMILASLAYGFHV
                     PMTQVLVEGIRNLNGMDVRYAIEFGYRIKLLAVVRRDGDAVEVRVHPTLVPLDHMLAS
                     VSHVFNAAMVRGDLSGDTLYYGRGAGREPTASTVIGDIGDIVRNLMAGKSRYRRGVCL
                     AEDDGRLRMRPAGDISSRYYVRLMVCDKAGSLGVITTLLGRHGVSILAATQKALSGAR
                     DTGYVPVVILTHAARGADIEEALRGIRAAGVLNEEPVKLRML"
     gene            1875..3227
                     /locus_tag="PLZ74_09450"
     CDS             1875..3227
                     /locus_tag="PLZ74_09450"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOR98586.1"
                     /translation="MKVCKFGGSSVANAEQVNKTVDIVLADPERRIVVVSAPGKRDKE
                     DTKVTDMLIACAELALAEKECETPLAAIVERYAEIQRDLGLSAGITAEIEKDLRFRLA
                     GDRSHRGSFLDSMKAAGEDNCAKIVAQALQARGVKAAYLNPQQAGLLLTNDYGNAQLI
                     EESYQNLAQALRDRGEIVVFPGFFGYTREGRVATFPRGGSDITGSILAAAVKAEVYEN
                     FTDVDSVYPVDPRLVPEVKAGIPVMTYREMRELSYAGFGVFHDEAVIPAVKAGVPIQV
                     RNTNHPEEPGTLIVQSRRALAGGVVGIASDDGFCNIFVDKYMMNREIGFGRHLLQILE
                     WEGVSYEHMPSGIDNVSVVIRDRNFDSARERRVLDRIRRDLNPDAVLVERGYALIMVV
                     GEGLYYTVGMASKATRALADAGVNIEMMNQGSSEISMMFGVKAEQRAKAVKALYQAFF
                     "
     gene            3227..4822
                     /gene="cimA"
                     /locus_tag="PLZ74_09455"
     CDS             3227..4822
                     /gene="cimA"
                     /locus_tag="PLZ74_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOR98587.1"
                     /translation="MEKIFLYDTTLRDGAQGEGISFSDTAKVRFARLLDDFGIDYIEG
                     GFAGSNPRDRKFFEDIRRERLSHARITAFGSTRRVNLDAASDPQIAGLLAADTEWVTI
                     YGKAWLLHVRDVLRATGPQNLQIIADTVGHLRQNGRQVFFDAEHFFDGYKDDPAYALQ
                     ALDAAVREGAAAIVLCDTNGGTLPHEIFTITGEVCAAFPGVEVGIHTHNDSNLAVAAT
                     LEGVRGGARQVQGSVNGYGERAGNANLISILPALELKMGYACVGPERLKKLCEVSRIT
                     DDLANQRPDPKQPYVGRSAFSHKAGAHVNAIQKNPRTFEHIAPEAVGNSRHVLVSELA
                     GVANVQIKAAELGIDLAALDRSQIREILNEIKSRENKGYSYESADGSFKILLQKVLNT
                     HKPFFELEGFRVIVEKRGSGEPCLSEATVKVKVRGETELTAGEGLGPVDALNAALRHA
                     LNRFYPEIEQITLTDYRVRILDPQEATRAVTRVLIESGDGTRHWGTVGVSENIIEASW
                     QALLDSVEYKLYEDRAIQIPPAP"
BASE COUNT          962 a         1513 c         1645 g          803 t
ORIGIN      
        1 gacagcatgt tgtcgaactg agtcacacaa acgaaaggac aacgcaatgg ccacaaagaa
       61 agcagcccct gcaaagaaga aagcagctcc tgctaagaag gcggctcctg ctaagaaggc
      121 ggctccgaag aaagccgctg tgaagaaagc tcctgccaag aaagcggctc cgaagaaagc
      181 cgttgtgaag aaggcggccc cgaagaaggc cgctgtgaag aaagctcccg ccaagaaggc
      241 tgcgccgaag aaagcggtga agaaggttgc cgcgaaaaag gcgaagaagt aaggggctct
      301 cctgaaagaa cgacactccc gcagctgtgg ctgcgggagt gttttttttg gtttcggtgc
      361 ctcccgaggc gtcctgaccg cttgcggcgg tttttgcggg cttgtaagcg ggtcgctgtt
      421 tctactatag taaaaattct ttttaaagaa aaaggaatca ggaaagcagg atgtgtatga
      481 agcagatcgg tgtaggcctt ttgggtttcg ggaccgtggg cgcgggtgtg gccgacggct
      541 tgcagcgtaa ccgcaaggtg atggccaggc ggctgggtgc cgacgtggtg ctgaagcgga
      601 tcgcggatct cgataccgtg accgaccgcg gtgtggccat cgatcccgcg atcctgactc
      661 ccgacgccct gtcggtgatc cacgatccgg agatccggat cgtcgtcgag ctgatcggcg
      721 gcacgggcgt tgccaaggaa ctggtgctgg cggcgctcaa cgcggggaag ccggtggtca
      781 ccgccaacaa gaaactgctg gccgaacacg gggaggagat tttcagtgcc gcggaacgca
      841 agggcgtgga catctatttc ggtgccagtg tgggcggcgg gatcccgatc atccgcgtgt
      901 tgcgcgaagg tctggcgggt aacgagattc agagtatcca cggcatcctc aacggcacgt
      961 gcaactacat cctcacccgc atggagcagg aggggcttcc gtttgacgcg gtgctgcggg
     1021 acgcgcagca gaagggttac gccgaagcgg acccgagtct ggacatcgac gggttcgaca
     1081 cggcccacaa ggccatgatc ctcgcctcgc tggcctacgg gttccatgtg ccgatgacgc
     1141 aggtgctcgt cgagggcatc cgcaacctca acggcatgga cgtgcgctac gcgatcgagt
     1201 tcggataccg catcaaactg ctggccgtgg tgcgccgcga cggggatgcg gtcgaggtgc
     1261 gcgtccaccc cactctggtg cctctggacc acatgctggc atcggtcagc catgtcttca
     1321 acgcggccat ggtgcgcggc gatctgagcg gcgacacgct ctattacgga cgcggcgctg
     1381 gccgcgaacc cacggccagc acggtgatcg gcgacatcgg cgacatcgtg cgcaacctga
     1441 tggccggcaa gtcgcggtac cggcgcggcg tctgcctcgc cgaggacgac ggccgtctgc
     1501 ggatgcgtcc ggcaggcgac atttcgagcc gctactacgt ccggctgatg gtgtgcgaca
     1561 aggccggttc actgggcgtc atcacgacgt tgctgggccg gcacggcgtg agcattctgg
     1621 cggcgacgca gaaggcgctg tccggggcgc gggataccgg atatgtgccg gtggtgatcc
     1681 tgacgcatgc ggcgcggggc gcggacatcg aggaggcgct gcgcgggatc cgcgcggcag
     1741 gcgtgctgaa cgaagagccg gtgaaattga ggatgctgta aaggcaaggg cgggcaaggc
     1801 gcgttccccg gacgcgttgc ggacagctcg gggagtcgcc cctgccgaag cgggcggggt
     1861 ttgaggagag cgtgatgaag gtatgtaaat tcggcgggtc atccgtcgcc aatgcggagc
     1921 aggtcaacaa aaccgtggat atcgtgctgg cggatccgga acggcggatc gtcgtggtct
     1981 ccgcccctgg caagcgcgac aaagaagaca ccaaggtgac cgacatgctg atcgcctgcg
     2041 cggaactcgc cttggcggaa aaagagtgcg agacgccgct cgcggcgatc gtggagcggt
     2101 acgccgaaat ccagcgcgat ctggggctgt ccgctggcat cactgcggag atcgagaagg
     2161 atttgcgctt ccgtctggct ggcgaccgtt cccaccgcgg cagttttctc gacagcatga
     2221 aagccgcggg agaggacaac tgcgcgaaga tcgtggccca ggccctgcag gcgcgcggcg
     2281 tcaaggccgc atacctcaac cctcagcagg cgggtctgct cctgaccaac gattacggca
     2341 atgcgcaact gatcgaggag tcgtaccaga atctggccca ggccctgcgg gaccgcgggg
     2401 aaatcgtggt cttccccggc tttttcggct acacccggga agggcgggtg gcgactttcc
     2461 cgcgcggcgg ttcggacatc accggctcga tcttggccgc ggcggtcaag gcagaggtct
     2521 acgagaactt caccgatgtg gactccgtct atcccgtgga cccgcggctg gtgccggagg
     2581 tcaaggccgg catcccggtc atgacctacc gcgagatgcg tgaactttcg tacgccgggt
     2641 tcggcgtgtt ccatgacgag gcggtgattc cggcggtcaa ggccggcgtg ccgatccagg
     2701 tgcgcaacac caaccatccg gaggagcccg gcaccctgat cgtgcagtcg cgccgcgcgc
     2761 ttgccggggg cgtggtcggc atcgcgagtg atgacggctt ctgcaacatc ttcgtggaca
     2821 agtacatgat gaaccgcgag atcggcttcg gccgccacct gctccagatc ctggagtggg
     2881 agggggtttc ctacgaacac atgccctcgg gcatcgacaa cgtctcggtg gtgatccgcg
     2941 accggaactt cgattccgcc cgcgaacggc gcgtgctcga ccgcatccgg cgcgacctca
     3001 accccgacgc ggtgcttgtg gaacgcggct acgcgctgat catggtggtg ggcgaagggc
     3061 tctactacac ggtgggaatg gcctccaaag ccacccgggc gctcgcggac gcgggcgtca
     3121 acatcgagat gatgaaccaa ggctcatccg aaatcagcat gatgttcggc gtcaaggccg
     3181 agcagcgcgc caaagcggtg aaggccctct atcaggcatt cttctaatgg aaaaaatctt
     3241 cctctacgac accaccctgc gcgatggcgc ccaaggcgag gggatctcct tctcggacac
     3301 ggccaaagtg cgtttcgcgc ggctgctgga tgatttcggc atcgattaca tcgagggcgg
     3361 gtttgccggc tccaatccgc gcgaccgcaa gttcttcgag gacatccggc gggaacgcct
     3421 cagccacgcg cgcatcacgg ccttcggcag cacgcgccgc gtgaacctcg acgcggccag
     3481 cgatccgcaa atcgctggcc tgctcgcggc cgataccgag tgggtcacca tctacggcaa
     3541 ggcctggctg ctgcatgtgc gggatgtcct gcgcgccacc ggcccccaaa acttgcagat
     3601 catcgccgac accgtcggcc acctgcggca aaacggacgc caggtctttt tcgacgcgga
     3661 acattttttc gacgggtata aggatgaccc cgcctacgcc ctgcaggcac tcgacgcggc
     3721 ggtgcgcgaa ggtgccgcgg ccatcgtgct gtgcgacacc aacggcggta cgctgccgca
     3781 cgagattttc acgatcaccg gcgaggtctg cgcggccttt cccggcgtcg aggtcggcat
     3841 ccacacccac aacgacagca acctcgcggt cgcggccacc cttgaaggcg tccgcggcgg
     3901 cgcgcgccag gtgcagggtt ccgtcaacgg gtacggcgag cgcgcgggca acgccaacct
     3961 catcagcatc ctgcccgcgc tcgaactgaa gatgggatac gcctgcgtcg gccccgagcg
     4021 gctgaagaaa ctctgcgagg tctcgcggat caccgatgac ctggccaacc agcggcccga
     4081 ccccaaacag ccttacgtcg ggcgttcggc cttcagccac aaggctggcg cgcacgtgaa
     4141 cgcgatccag aaaaacccgc ggaccttcga gcacatcgcg cccgaggcgg tcggcaacag
     4201 ccgccacgtg ctggtctcgg agctcgccgg cgtggccaac gtgcagatca aggcggctga
     4261 actgggtatc gacctcgccg ccttggacag aagtcagata cgcgagatcc tgaacgagat
     4321 caagagccgc gagaacaaag gctactccta cgaatccgcg gacggctcgt tcaaaatcct
     4381 gctgcagaag gtgctcaaca cccacaagcc ctttttcgag ctggaggggt tccgcgtgat
     4441 cgtcgagaag cgcggcagcg gcgagccctg cctctccgaa gccacggtca aagtcaaagt
     4501 ccggggcgag accgaactca cggcaggcga gggcttgggg cccgtggacg cgctgaacgc
     4561 cgcgctgcgc catgccctga accgtttcta ccccgagatc gaacagatca ccctcaccga
     4621 ctaccgcgtc cgcattctgg atccgcagga agccacgcgg gccgtcacgc gcgtgctgat
     4681 cgaatccggg gacggcacgc ggcactgggg cacggtcggc gtgtcggaga acatcatcga
     4741 agcctcgtgg caggcgctgc tcgacagcgt ggagtacaaa ctgtacgagg accgcgcgat
     4801 acagatcccc cctgcaccgt gatgggtttg acgggaggga cgagcgtctg cccgtccggg
     4861 caagcagacg cttgcccttc ccgccggggc cgctgtcggg atcgggaatg ttgccccgcg
     4921 acc
//
LOCUS       DAOSRB010000202         4887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15729_length_4887_cov_6.161010, whole genome shotgun sequence.
ACCESSION   DAOSRB010000202 DAOSRB010000000
VERSION     DAOSRB010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15729_length_4887_cov_6.161010"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLZ74_09460"
     CDS             complement(<1..289)
                     /locus_tag="PLZ74_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOR98588.1"
                     /translation="MRTKKDSGRGRMEDEDLAIIYIDEPGCRVLDLAKAGLREVPMLN
                     FSRRRHFRGPTSPHRHAGCLEVGICLRGALTLLNNNREYRVMPGDLFLNKPS"
     gene            688..4782
                     /locus_tag="PLZ74_09465"
     CDS             688..4782
                     /locus_tag="PLZ74_09465"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOR98589.1"
                     /translation="MALIGVGVLAFAEQSEPAGCAPLPVTWTPFAREGAVRQQVVMTA
                     TVGTDRGKALDVVNFHAGDKRVMVSGEDAKVNCNEPMLFGRLVVYLGTRHWLVLNLGP
                     AASVKTDRPDELTVDRAAGTATWRREYNVPGTDGETAVFSYRVSGAADSKVNVDYDFG
                     VPQEKANRIGQKFHSYTWCNINYGGDVTKNVFGAGDVPFQPIDIAAFTPLMQKNPEKH
                     HLQTGGKHAGDVRFFHEPDNEELHFEIFFPTNALASGPWYHMLHRTGPTWDTYRRGVF
                     PVVGLSHFSADPARTRPVKGRIVIDLCMSSVAKHAAEPPVGGIDFWASDALHVPPRPT
                     RNLLVNGSFEQGLKHWRWEDGGSVWDPPAEGDREKYAVVGGGLFGAKMMELNASQPRM
                     CGICSAALPLEAGKRYTVSAWFKARGEKNGYFSLWPKSVAMRGNRAFMKFLPDPEKLR
                     CHWNIKPEEGWKRVEYTFSPDSSGIYLQLGGANVFVDGIQVEAGVEATANVDDPVIAH
                     LVTSDPDNDLVPDEDYAGHLAFIGAADQSFDVRVRVANVYHETCVDERLSATLDAAGR
                     AEMPFALPPERLGEGVFTVRYDFRLPDGCAWTDYERFSVMVPCNATHATKRFFCSHMW
                     FGKHARTPHMARKFVEWGFGSVNGVGNDTLTTSTAARFFRDYDIQNIVHPVAYERLTQ
                     TNAVLRAAALAAHPELAEKKSFSPFRYIEKATPEVQAALESCAYELAKNCATNDLVWT
                     YWNEDEDCARRIGFDEYFKLQYAVWKGAKRAFDERGLKLGFTPTHGTSHYFRGRNYDV
                     IDGYLETAQKHGFSYSAVSIHPYSNIDGGILGPRDADVETQHLIARMAHYGYAPDVPV
                     YFTECFNMLPMNIPEWGAHGWGDRFRGQQMPSLAAGVREFTHAASLMRLYLIALKFWP
                     KVRMAHPWQSAPVFDLAFSPRGWSKVPNTLARLLPDPRWAGDAQPFGDVRGYCFNQKR
                     EDGSTQAILAVWTTNHDAELGRRKGPVVQMALPAGTAAYDMNGNRRALPKPVDGRYEI
                     PLTPAPLFLAGRDAAALLQALEDAASDDPATALVVDVRPDERGVLSLVVDNVTKKTQE
                     GELTVNGRSLPYAIPARGTEVFPIVETPDPQAMTLYTWEGHISTLSKPWRAQWFYVDE
                     CGAVPDWASVRAIPLVNEVRAEDGRPVAMKAAFKAAYNADFFFLRVDVEDPAFVPLAE
                     SQQPFRPQHLYAYDGCLEVFFDGFADARSQGEKDYDENDMRYDFAQGHCWRQRAVNWQ
                     LAQGTQSATDEEIRTKMQHTFTRTAQGYVYEIAIAKRYMAPVDLKPGTVAGLGLYLHD
                     YVKPDERVERGLSFAVESGATCDRKPYLWPTMILRSGSGSQPRE"
BASE COUNT          928 a         1498 c         1542 g          919 t
ORIGIN      
        1 cagacggctt gttgaggaag agatcgcccg gcatgacccg atactctcga ttgttgttca
       61 ggagggtgag tgcgccgcgc aggcagatac cgacctccag acagccagcg tggcggtggg
      121 ggctggtcgg cccgcgaaaa tggcggcgac ggctgaagtt aagcatcggg acttcgcgta
      181 gcccggcttt ggccagatcg aggacacggc accccggctc gtcaatgtag atgatagcca
      241 agtcctcatc ttccatgcgc ccgcgcccgc tatccttttt cgtcctcatg aaggcatcta
      301 agtgtccagc accattttcg tgttgtcgat agcttcccac attcccgtat gccctgtcaa
      361 tcttcacagt tacggacaat acaacaattt tacatttctt ttttcattgc ttcttttgtg
      421 ccatgggtac ggtatgctat tgttacgaat ggcgacaggc taccgggctt cggtcatgcg
      481 ccgcgggtgc aggtgggcag gaacgagaag gaaaaaagga gggtgaacat ccctccagaa
      541 ggtagcgcat gaaggtgaga gggtcatgtt ttgagacatg gacgggtgtg aggatggcgg
      601 gatgtctttg cgcctggaag caatcctctc gtgtggtcta attatgtcct tgaaatgaga
      661 cactacccga agaagtcgtt tttcataatg gcgttgatcg gtgtcggcgt tttggcgttt
      721 gccgaacagt ctgaacctgc gggctgtgcg ccgctgcccg tgacgtggac accgttcgcg
      781 cgcgaaggcg ccgtgcgcca gcaggtggtg atgaccgcga ccgtcggcac tgaccgcggc
      841 aaggctttgg atgtggtcaa cttccatgca ggcgacaagc gtgtgatggt gagcggtgag
      901 gacgccaagg tgaactgcaa cgagcccatg cttttcgggc ggctcgtcgt ctatctgggt
      961 acgcggcact ggctcgttct caacctgggc cccgccgcga gcgtcaagac cgatcgcccc
     1021 gacgaactca cggtcgaccg tgccgccgga accgccacat ggcgccgcga atacaacgtg
     1081 ccgggcacgg acggagagac ggccgtcttc tcctaccgtg tctccggcgc ggccgattcc
     1141 aaggtgaacg tggactacga cttcggcgtg ccgcaggaga aggcgaaccg aatcggccaa
     1201 aagttccaca gctacacctg gtgcaacatc aactacggcg gcgacgtgac gaagaacgtc
     1261 ttcggcgcgg gcgatgtgcc gttccagccc atcgacatcg cggccttcac ccccctcatg
     1321 cagaagaatc ctgaaaagca ccacctccag acaggcggta agcatgcggg cgatgtgcgt
     1381 ttctttcacg agcctgacaa cgaagagcta catttcgaga tctttttccc cacgaacgct
     1441 ttggcgtccg gtccttggta ccacatgctg catcggacag ggcctacatg ggacacctat
     1501 cggcgcggcg tgttcccggt cgtggggctg agtcacttca gcgccgatcc ggcgcggacg
     1561 cgtcctgtga agggacgcat cgtgatcgac ctctgcatgt cctccgtggc gaagcacgcc
     1621 gccgagccgc ccgtgggggg catcgatttc tgggcgtccg acgccttgca cgtgccgccg
     1681 cggcccacgc gcaacctgct ggtgaacggc tccttcgagc agggactcaa gcactggcgc
     1741 tgggaggacg gcgggtccgt ctgggacccg ccggccgagg gcgacaggga gaagtacgcc
     1801 gtggtgggcg gcggcctgtt cggcgcgaag atgatggaac tcaacgcatc gcagccgcgc
     1861 atgtgcggaa tttgctcggc ggcgctgccg ttggaggcgg gcaagcgtta cacggtgagc
     1921 gcctggttca aagcgcgcgg cgaaaagaac ggatacttct cgctgtggcc caaatccgtg
     1981 gcgatgcgcg gcaaccgtgc gttcatgaag ttcctacccg acccggaaaa gctgcgctgc
     2041 cattggaaca tcaagcccga ggagggctgg aagcgcgtgg aatacacgtt ttcgcccgat
     2101 tccagcggca tctacctgca gctgggcggc gcgaacgtct tcgtggacgg catccaggtg
     2161 gaggcgggcg tcgaggcgac cgcaaacgtg gatgaccccg tcatcgcgca cctcgtcacg
     2221 tccgaccccg acaacgacct cgtgccggac gaagactacg ccgggcacct cgcgttcatt
     2281 ggcgctgccg atcagtcgtt cgatgtgcgc gtgcgtgtgg cgaacgtcta tcatgaaacg
     2341 tgcgtggatg aacggctgtc tgccacactc gacgccgctg gccgcgctga gatgccgttc
     2401 gccctgccgc ctgaaagact cggcgagggc gtcttcaccg tgcgctacga tttccgttta
     2461 ccggatggct gcgcatggac ggactacgag cgtttttcgg taatggttcc ctgcaacgcc
     2521 acgcacgcca cgaagcggtt cttctgctcg cacatgtggt tcggcaagca cgcccgtacg
     2581 ccgcacatgg cgaggaaatt cgtcgagtgg ggattcggtt cggtcaacgg cgtcggcaac
     2641 gacacgctca cgacatccac ggcggcgcgc ttcttccgcg attacgacat ccagaacatc
     2701 gtgcatcccg ttgcgtatga acgcctcacg cagacgaacg ccgtcttgcg cgcggcggct
     2761 ctcgcggcgc atcccgaact ggcggagaag aaaagcttca gcccgttccg gtacatcgaa
     2821 aaagccacgc cagaggtaca ggccgccctg gagtcatgcg cttacgaact tgcgaaaaac
     2881 tgcgccacga acgacctcgt gtggacgtac tggaacgagg atgaagactg cgcccgccgc
     2941 atcggtttcg atgaatactt caagctgcag tatgccgtgt ggaaaggcgc gaaacgcgcg
     3001 ttcgatgagc gcgggctgaa actcggcttc acgcccacgc acggcacgag ccattacttc
     3061 cgcgggcgca actacgatgt gatcgacggc tatctcgaaa cggcccaaaa gcacggtttt
     3121 tcgtattccg ccgtctcgat ccacccctac tccaatatcg acggtggcat tttggggccg
     3181 cgcgacgccg acgttgagac gcagcacctg atcgcgcgca tggcccatta cggctacgcg
     3241 ccggatgtcc ccgtttattt caccgaatgc ttcaacatgt tgccgatgaa catccccgaa
     3301 tggggcgcac acggctgggg cgaccgtttc cgcggccagc agatgccctc gcttgccgcg
     3361 ggcgtgcgcg agttcacgca cgcggcgagt ttgatgcgcc tctacctcat tgcgctcaag
     3421 ttctggccga aagtgcgcat ggcgcatccg tggcagagtg cgccggtgtt tgacctcgcc
     3481 ttctctcccc ggggctggag caaggtgccc aacactctgg cgcgcctgct gcccgatccg
     3541 cgctgggcgg gcgacgcgca gccgttcggc gacgtgcgcg ggtattgctt caaccagaag
     3601 cgggaggacg gttccacgca ggcgatcctc gccgtgtgga ccacaaacca tgacgccgaa
     3661 ctcggtcgtc gcaaaggccc tgtcgtacag atggccttgc cggccggcac ggccgcctac
     3721 gacatgaacg gcaaccgccg cgccctgccg aaaccggtgg acgggcggta cgagattccg
     3781 ctcacgcctg cgccgctctt ccttgcgggc cgcgatgcag cggcgctgct gcaggcgctg
     3841 gaggatgccg cctctgacga tcccgccacg gccctcgtcg tggacgtgcg ccccgacgag
     3901 aggggcgtcc tgtcgcttgt cgtggacaac gtcaccaaga agacgcagga gggcgagctc
     3961 accgtgaacg ggcgcagcct gccctacgcg atccccgccc gaggcacgga ggtcttcccg
     4021 attgtcgaaa cgcccgaccc gcaggcgatg acgctctaca catgggaagg gcacatctcc
     4081 acgctctcca agccctggcg cgcgcagtgg ttctacgtgg acgagtgcgg cgcggtgcct
     4141 gactgggcgt ctgtccgcgc gatccccctt gtgaacgagg tgcgcgcgga agacggcagg
     4201 cccgtcgcga tgaaggccgc gttcaaggcg gcgtacaacg ccgacttctt cttcctgcgc
     4261 gtagacgtgg aggatcccgc gttcgtcccg ctcgccgagt cgcagcagcc gttcaggccg
     4321 cagcacctgt atgcctacga cgggtgcctg gaggtgttct ttgacggttt cgccgatgcc
     4381 cgcagccaag gcgaaaaaga ttacgacgag aacgacatgc gttacgactt cgcgcaggga
     4441 cattgctggc ggcaaagggc ggtgaactgg cagctcgcgc aaggcacaca gtcggccacg
     4501 gacgaggaga tccgcacaaa gatgcagcac acgttcacgc gcacggcgca gggttacgtg
     4561 tacgagatcg cgattgcgaa gcgctacatg gcgcctgtcg acctcaagcc gggcacggtg
     4621 gcgggtttgg ggctttacct tcatgattac gtgaaaccag acgaacgcgt tgaacggggg
     4681 ttgtccttcg ccgtggaatc tggagccacc tgtgaccgca agccctatct ctggcccacg
     4741 atgattttac ggtcaggtag tggttcgcaa ccgcgagagt aaaaagacag gtattcgcat
     4801 tcctgtttgt ggcttaaacc taaaaagagc agttgaacct tcacctttct ttggtttcat
     4861 ccaaatcggg gtcgggatcg ctatcgg
//
LOCUS       DAOSRB010000203         4850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15899_length_4850_cov_7.038165, whole genome shotgun sequence.
ACCESSION   DAOSRB010000203 DAOSRB010000000
VERSION     DAOSRB010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15899_length_4850_cov_7.038165"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="PLZ74_09470"
     CDS             <1..1690
                     /locus_tag="PLZ74_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR98590.1"
                     /translation="RANPRAAATGKLPAWFDRMEAVGNRFGLSFRDEEGRMTGELLLI
                     ALVVVPLVMLVKMTALYLNHYFLRWTGAKVVQDFRIELFSHLQKQSLGFFGKVDVGQL
                     ISRCTSDPHQVDNVIAHTLADLCRAPFEILFSIGFVIYFAIRNNMIETLVLVLVGFPL
                     VLFPMAVLGSWVRRWSRRSLLRISYVVSKIHENLTCIRVVKAYHTEAYEIEKYRKANH
                     FYMKSVLRAVRIELLITPAVECVAIFLCFAFITYCYFRRLTIDQIAPLLVPILVAYRP
                     MKQLGKVQAQLERGRAALARMYSLLDLDMSLPMAARPVRKEQFEDAVRFDHVGFRYGD
                     GPDLTIRDLSFVLPRGSLVAVVGGTGSGKTTVANLLARFYDPVSGSVTMDGVDLRQIE
                     IGDLRKLIGVVTQETVLFNDTIENNIRYGMPGTTREQVVAAAQMANAHTFITDHPEGY
                     DRVVGEKGFVLSGGERQRVAIARAILKNPPILILDEATSALDTVTERLVQDAINRLMA
                     NRTTFAIAHRLSTIRNADQILVMEKGAVIERGTHEELWAAGNKYRALCDMQMME"
     gene            1734..3347
                     /locus_tag="PLZ74_09475"
     CDS             1734..3347
                     /locus_tag="PLZ74_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOR98591.1"
                     /translation="MGGQKASLEKQIVKAGFSILVAHVLFKLAGLIQSVVMGRYLPPG
                     VFDVVYVFAFERCVYMLFLIGEEVLGPSLLPVFMRELDTKSEHDAWQFANTILTLQFF
                     VLAVVAALLCVAPQAVVAFLTQWGPTESPEKFELAARSVRTLAPALVGLSLGSTTYVL
                     LNGYKRFFLAAFGDAVWKFCVVGFLIVGTLFSREGAQMLMWGLVAGSVCKLVVHLFGL
                     HDKLRYIRPRLAWSHPAIRTFCLLALPLLAGILIAKVRDQVNDVYVLSALDESGLMQA
                     NSMGRKLQSTLLYLVPYTLSIAVFPFFCELVDKQDQVGLGQLVTRFGRMLLSVFVPFA
                     IFVAVAAVPITSLIFRGGYFDATAVQRTSVSLSFYTFVLPAAAIEMLVMQAFFANRRM
                     LSVMWIGVVFSILSMGISYIGLQVADGRELVVLAFIAGGFTLARTLKSLTLVMMLRAN
                     ARVFPFRETAVFLSRVTVAAGLAALLGWLTLHAQPLAELAGRVGDLVRLTAAGAVFGC
                     VYLGGTYVLKISEIRELCGLAIQKLSKHA"
     gene            3442..3759
                     /locus_tag="PLZ74_09480"
     CDS             3442..3759
                     /locus_tag="PLZ74_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOR98592.1"
                     /translation="MGMLDQVKQAMQMRKEAKRIQSEIEKITCEYANGGITCVARGDF
                     TIVSLKVTQEALADVIAGKPERFETMLGNVVNGALKGVKKQTQDAMTKMMQGTGMGNM
                     FGG"
     gene            3767..4387
                     /gene="recR"
                     /locus_tag="PLZ74_09485"
     CDS             3767..4387
                     /gene="recR"
                     /locus_tag="PLZ74_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOR98593.1"
                     /translation="MVPPVDNLIRALSKLPGLGRRSAERAALALLRKPELLLDTLMRA
                     LHEARETVCCCSLCGGFTAKTCEPCALCTDPMRDDSLLCVVEEPADIVSIERSGGFRG
                     RYHALMGKLSPSKRTGPSDLRLVALERRVSKGPVREVLLALSTDMEGDATAGYIGEML
                     KPSGVRITRLAFGLPADSGVGYSDPLTLKRAISGRVVCEPQEVTEP"
     gene            complement(4374..4517)
                     /locus_tag="PLZ74_09490"
     CDS             complement(4374..4517)
                     /locus_tag="PLZ74_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98594.1"
                     /translation="MGFAGPELGSAKDWTVEGLKRDYRTYVDESGKRVLPGSPRGTVI
                     TVR"
BASE COUNT          836 a         1323 c         1582 g         1109 t
ORIGIN      
        1 ccgcgcgaat ccccgcgcgg cggctacggg aaaactgccg gcctggttcg accggatgga
       61 ggccgtgggt aaccgcttcg gactctcctt ccgggatgag gagggccgga tgacgggcga
      121 actgctcctg atcgccttgg tcgtggtgcc gcttgtcatg ctggtcaaaa tgacggcgct
      181 ctatctgaac cactacttct tgcgctggac cggcgcgaaa gtggtgcagg atttccgcat
      241 cgaactcttt agccacctgc agaaacagtc gctcggtttt ttcgggaagg tcgatgtcgg
      301 ccaattgata agccgatgca ccagtgaccc gcaccaagtt gacaatgtga tcgcgcacac
      361 actggccgat ttgtgccgtg ccccttttga aattctgttt tccatagggt ttgtgatcta
      421 tttcgcgatt cggaacaaca tgatcgaaac gctggtgctg gtgctcgtcg gcttcccgtt
      481 ggtgctgttc cccatggcgg ttctggggtc ttgggtccgc cgctggtccc ggaggtcgct
      541 gctccggatc tcttatgtgg tgtccaagat ccatgagaac ctgacctgca tccgcgtggt
      601 caaggcgtat catacggagg cgtatgagat tgagaagtac cgcaaggcga accacttcta
      661 tatgaagagt gtgttgcggg ccgtgcggat cgagttgctg atcactccgg cggtggagtg
      721 cgtagcgatt ttcctctgtt tcgcttttat cacctactgc tatttccggc gactgacgat
      781 cgatcagatc gcgccgctgc tggtgccgat tctggtggcc taccgtccga tgaagcaact
      841 cggcaaggtt caggcccagc tggagcgcgg ccgcgctgcc ctggcgcgga tgtactccct
      901 gcttgatttg gatatgagtc tgccgatggc ggcccggccg gtccgcaaag aacagttcga
      961 ggatgccgtc cgctttgacc acgtcgggtt ccggtatggg gacggacctg acctcacgat
     1021 ccgcgacctc tcgttcgtgt tgccgcgtgg ttcgctggtg gcggtggttg gagggaccgg
     1081 ctccggcaag acgaccgtgg ccaatctgct ggcgcggttt tacgatccgg tttccgggtc
     1141 cgtgaccatg gacggggtgg atctgcggca gatcgagatt ggggatctgc gtaagctgat
     1201 cggcgtggtg acgcaggaga cggttttgtt caacgacacc attgagaaca atatccggta
     1261 cggcatgccg ggaacgaccc gtgaacaggt ggttgccgca gcccaaatgg ccaacgcgca
     1321 cacgttcatc acagaccatc cggaagggta tgaccgtgtg gtgggagaga aggggttcgt
     1381 gctgagcggc ggggaacgcc agcgtgtggc gatcgcccgc gctattctca aaaacccccc
     1441 catcctgatt ctggacgagg cgaccagcgc attggacacg gtgaccgaac ggttggtgca
     1501 ggatgcgatc aaccggctca tggccaaccg gaccacattc gccatcgccc accgtttgag
     1561 taccattcgg aatgcggatc aaattctggt gatggaaaag ggggccgtca tcgaacgcgg
     1621 tacgcacgag gagttgtggg ccgcgggcaa caagtaccgc gcattgtgcg acatgcagat
     1681 gatggagtga ggggtatcgg aggggccatc gcccgtttcc gtttggggga cgcatggggg
     1741 gacaaaaggc gagtctcgag aaacagatcg tcaaggcggg tttttccatt ctggtggccc
     1801 acgttttgtt taagctggcg gggctgatcc agtccgttgt catgggccgt tatcttccgc
     1861 ccggtgtgtt cgatgtggtg tacgtgttcg ccttcgagcg gtgtgtctat atgcttttcc
     1921 tcatcggcga ggaagtgctg gggccgtcgc tgttgccggt tttcatgcgc gagttggaca
     1981 cgaagtcgga acacgacgca tggcagttcg ccaacacgat cctgaccctg cagttcttcg
     2041 tgctggcggt cgtcgcggcg ttgctgtgcg tcgcgcccca agcggtggtc gcgtttctca
     2101 cgcagtgggg accgaccgaa tcgccggaga agttcgagtt ggccgcgcgc agcgtcagga
     2161 ccctggctcc ggctctggtg gggctttcat tggggtcgac cacctacgtg ctgctgaacg
     2221 gatacaagcg gttctttctg gccgcgttcg gcgatgcggt gtggaagttc tgcgttgtcg
     2281 ggttcctcat agtcgggacg ctgttttcgc gcgagggagc ccagatgctg atgtggggcc
     2341 ttgtggccgg ttcggtttgc aagttggttg tgcatctgtt cgggcttcac gacaagctgc
     2401 gatatatccg gccaaggctg gcatggtccc atccggcgat ccggaccttc tgtttgctgg
     2461 cgcttccgct gctggccggc atcctgatcg ccaaagtccg cgatcaggtc aacgatgtct
     2521 atgtgttgag cgcgctggat gaatcgggcc tcatgcaggc gaactcgatg gggcgcaagc
     2581 tgcagagcac gttgctctat ctcgtgccgt acacgctgtc gatcgcggtt ttcccgtttt
     2641 tctgcgaact ggtggataag caggatcagg tggggttggg acagttggtc acgcgctttg
     2701 gccggatgtt gctctccgtg tttgtgccct tcgcgatctt tgtggctgtg gcagcggtgc
     2761 cgatcacctc gctgattttc cggggcgggt attttgacgc gacagcagtt cagcggacgt
     2821 cggtttcgct ttccttctac acgttcgtcc tgccggccgc ggcgatcgag atgctggtga
     2881 tgcaggcgtt tttcgcaaac cgcaggatgc tttccgtgat gtggattgga gtggtgttct
     2941 ccatcctcag catggggatc agttatatcg gtctccaagt cgcggacgga cgcgagttgg
     3001 tcgtgctggc cttcatcgca ggcggcttca cgctggcccg cacactgaag agtctgacgc
     3061 tggtcatgat gctgcgggcg aatgcccggg tctttccgtt tcgcgagacc gcggttttcc
     3121 tttcgcgtgt caccgtggcg gccggtctcg ccgccctttt gggatggttg acgctccatg
     3181 cccagccgct tgcggaactg gcggggcgcg tgggggatct ggtgcgcctg actgcggcgg
     3241 gagccgtttt cggttgcgtc tacctcggag gcacctacgt tctgaaaatc agtgagatcc
     3301 gcgaactctg cggtttggcg atccagaaac tcagcaaaca cgcttgagaa ttcagagccg
     3361 gatgtattac actctccctg tcttgtctcc cgtaccgggg tgtgggtgaa cggaatggaa
     3421 tcttgtaagg agtgatggaa catgggtatg ttggatcagg tgaaacaggc gatgcagatg
     3481 cggaaagaag ccaagcggat tcagtccgag atcgagaaga tcacttgcga gtacgcgaac
     3541 gggggaatca cctgtgtggc gcgcggcgat ttcacgattg tttccctcaa ggtgacgcaa
     3601 gaggcgctcg ccgatgtgat cgcgggcaag ccggagcgtt ttgagaccat gctgggcaat
     3661 gttgtcaacg gcgcgctcaa aggcgtgaag aaacagacac aggacgccat gaccaaaatg
     3721 atgcagggca cgggcatggg caacatgttc ggagggtaag ccagcgatgg tgccgccggt
     3781 cgataatctg atccgggccc tgtccaaact gcctgggctg ggcagaaggt cggctgaacg
     3841 cgcggctctg gccttgttgc gcaaaccgga gctgttgctg gacactctga tgcgtgccct
     3901 gcatgaggcg cgggagacgg tctgctgttg ttcgctatgc ggcggtttca ctgccaaaac
     3961 gtgtgaaccg tgcgcgttgt gcacggatcc gatgcgcgat gacagcctgc tttgcgtggt
     4021 cgaggaaccg gcggacatcg tttccatcga acgttccggc ggtttccgcg gccggtacca
     4081 cgccctgatg ggcaagcttt ccccatccaa acgtaccggt ccgtcggact tgcggctggt
     4141 cgcgctcgag cggcgcgtct ccaaagggcc ggtccgggag gtgctgctgg cactcagcac
     4201 cgacatggag ggtgacgcga cagccgggta catcggcgag atgttgaaac cttccggtgt
     4261 gcggatcacg cgtttggcct tcggtctgcc cgcggacagc ggtgtcggct attccgatcc
     4321 cctgaccctc aaacgagcga tctcgggtcg cgtggtctgc gagcctcagg aagttaccga
     4381 accgtgatca ccgtgccgcg cggagaaccc ggcagcaccc gttttccgct ttcatccaca
     4441 taggtgcggt aatcgcgctt caggccttcc acggtccagt cttttgcgga tcccaactcc
     4501 ggtcccgcga accccatcca gccctgaacc taaaaagagc agttgaacct tcaactctct
     4561 ttggtttcaa ggtttcaggg tttctggttt cagggtttca gggtttcatc caaatcggga
     4621 tcgggatcgc tatcggtatc gggatcggga tcgggaacgt cgacccgcga cattcgaccc
     4681 gcgacgatcg acagggacgc ccgcgtttcc gacaccgcag gtcgcttttc agggtttcag
     4741 ggtttctggt ttcagggttt cagggtttca gggtttcatc caaatcggga tcgctatcgg
     4801 gatcgggatc gctatcggga tcgggatcgg gaacgtcgac ccgcgacccg
//
LOCUS       DAOSRB010000204         4836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_15967_length_4836_cov_6.302866, whole genome shotgun sequence.
ACCESSION   DAOSRB010000204 DAOSRB010000000
VERSION     DAOSRB010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15967_length_4836_cov_6.302866"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="PLZ74_09495"
     CDS             complement(<1..1604)
                     /locus_tag="PLZ74_09495"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR98595.1"
                     /translation="MCGQQPFFLFSPGRALSATRFVPETLRGAVLFVPPFYEERKGAL
                     PAFTRTARALAEHQIASLLFDFSGCGDSAGDFGNTDPEWMEEDAETALTWLRNALPGL
                     PVALLGLRTGAVLAARLAARHPESALLVGWSPTDGPTFIRQLLQRRMVNDMTAYGKAR
                     ESRAALEERLRHGDAVDLDGYVMTGAFHAWLQCLLPPALRIPVRLFSGGHDPAASRTF
                     AAQAVHAECSELRFPPFWNTVGQVDLTALTDETVACLSACLSGPTRPAKSFPALADCP
                     DSTAEAACLTLPVSPKVRAMLDLPRGTPRAGALFLHGWSGDRTGPHRLFTRFGRLLAA
                     QGTLCLRPDFAGRGLSGGRPEEASIAGMAEGAQAALAALRGRLPPGTPISVVAICSGC
                     KVAITLAAGNPDLANLLLWSPESMGSLRSGATGWRKTRAALLAYARKLTRPETWRKIL
                     TGRVRTDLVTRALVRHETRSAEEAVREDATLARFRAFRNPLLLVFGGSDPDAPGSRTA
                     YTRYCRTHAIPCRTHTIPHAGHSYYG"
     gene            1728..2249
                     /locus_tag="PLZ74_09500"
     CDS             1728..2249
                     /locus_tag="PLZ74_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR98596.1"
                     /translation="MPLYEFYCEPCHTLYTFRSPRVDTVTLPVCPDCGQELKREISPF
                     SHSVRGKTCADTEEDPGDLDRMDVAAAKLSERLHALERDDADPREAVRVMRELAREGG
                     IRFNREVQEAMARIESGEDPEQVEETFREVFDTENPFEPDNESPGPATTGWWRRIHPP
                     RRDPVWRDLPERG"
     gene            complement(2306..3160)
                     /locus_tag="PLZ74_09505"
     CDS             complement(2306..3160)
                     /locus_tag="PLZ74_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOR98597.1"
                     /translation="MNDTEVRFFFLLFAVFSALFGACVGSFLNVCIHRIPRDESVVTP
                     RSHCPHCNTLIPWYLNIPVASWLALRGRCASCKGPIAFRYTLVELLTAALFLAVFLKW
                     AAPAALGTTAIPHPAAIPVYWLFLAGLVLGTFVDFEHFILPDSVTIGGMVAGPILSAL
                     VPSLHGRTVWWQGLLLSLIGLAVGFGLLYAVAWIGGRIFKKEAMGFGDVKLMGAIGAF
                     LGWQAVLFTVFVSSLLGSVCGLLMIAFGGVKLQSRIPFGPYLSAAALIWVFWGEVLFG
                     AYLRLMRG"
     gene            complement(3213..4586)
                     /locus_tag="PLZ74_09510"
     CDS             complement(3213..4586)
                     /locus_tag="PLZ74_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOR98598.1"
                     /translation="MKRCLLLASLACVGTLAAQTVYTSDWESIDKRPVPQWWCDAKFG
                     IFIHWGVYAVPAYAPTDVANVYAKYSEHYDNRMRHNNPAFTSFHARVYGPHVTYADFA
                     TRFRAEHFRPEQWAALFKKSGARYVVLTSKHHDGYALWPSDYSPRWNSQVLGPHRDLA
                     GELTDAVKAAGLHMGFYYSLLEWGNPLYTKETIGRWVAEMNLPQMKELVTRYKPDIVW
                     TDGEWDFTDVELRSTDFLAWLYNESPVKETVVVNDRWGKKLRSKHGGHYTTEYSHGSN
                     EANEKQPVVHPWEECRGIGGSFGFNRFETTEHYMTSAQCIETLIEKVSRGGNLLLNIG
                     PQADGLIPVIMEERLLDMGKWLAVNGEAIYGTTTWRHRPKDMGKNHIYFTRKPDALYV
                     LCTRWPQQPIAVSPVSGAAAVTLLGSDAPVAFTCAGDTITLRPPAINPGTAPCDHAWV
                     FKIAHPR"
BASE COUNT          921 a         1487 c         1602 g          826 t
ORIGIN      
        1 tcgccgtagt agctgtggcc cgcgtggggg atggtgtgcg tccggcaggg gatcgcgtgg
       61 gtccggcagt agcgcgtgta ggctgtgcgt gagcccggcg cgtcggggtc cgaaccgccg
      121 aaaaccagca gtagcggatt acggaacgcg cggaaacgcg cgagtgtcgc atcttcccgc
      181 accgcctcct ccgcgctccg ggtctcatgc cggactagcg cccgcgtgac cagatccgtc
      241 cgcacccggc cggtcaagat tttccgccag gtttcgggtc gggtcagctt gcgcgcgtaa
      301 gcgagaagcg cggcccgtgt tttacgccag ccggtggcgc cgctgcgcag ggatcccatg
      361 gattcgggtg accagagcag cagattggcg agatcggggt tgcctgccgc aagggtgatc
      421 gccaccttgc aaccggaaca gatggctacg acggaaatcg gcgtccccgg cggcaggcgg
      481 ccgcgaagcg cggccaacgc ggcttgcgct ccctccgcca ttccggcgat cgaggcctcc
      541 tccggccgac ctccgctgag tccccgtccc gcaaaatccg gacgcaggca cagggtgccc
      601 tgtgccgcca gcagacgtcc gaagcgcgta aaaagccggt gcggtccggt acggtctccg
      661 gaccagccgt gcaggaaaag cgcgcctgct ctcggcgtgc ctcgcggcag atccagcatg
      721 gcgcgtacct tgggcgagac gggaagcgtc aggcatgcgg cttccgcagt gctgtccggg
      781 cagtcggcca gcgccgggaa cgacttagcc gggcgcgtcg ggccggagag gcaggcggag
      841 aggcacgcca cggtttcatc ggtcagcgca gtcagatcga cctggccgac cgtgttccag
      901 aatggcggga aacgcagttc cgaacattcc gcgtgtacgg cttgtgccgc gaaggtccgg
      961 cttgccgcag gatcatggcc accggagaac agacggacgg gaatccgcag ggctggcggc
     1021 aacaggcact gaagccaggc gtggaacgcg ccggtcatga cataaccgtc gagatccacc
     1081 gcgtcgccgt ggcgcaaccg ctcctccagc gcggcgcggc tctcccgcgc cttcccgtaa
     1141 gcggtcatgt cgttcaccat gcggcgttgc aacagttgcc ggatgaaggt cggtccgtcg
     1201 gtcggcgacc agccgaccag cagcgccgac tccggatggc gcgcggccag ccgtgcggca
     1261 agcacggcac cggtccgcag accgagcagg gccacgggca gaccgggcag ggcattgcgc
     1321 agccacgtca gcgcagtctc cgcatcttct tccatccatt ccggatcggt gttcccaaag
     1381 tcgccggccg aatcgccgca acctgaaaaa tcaaacagca gcgaggcgat ctggtgttcc
     1441 gccagcgcgc gtgcggtgcg tgtgaaagcg gggagcgcgc ccttgcgctc ttcgtagaac
     1501 ggcggcacga acagcacggc gccgcggagg gtctccggca caaagcgggt cgcgctcaag
     1561 gcccggcccg gggagaaaag gaaaaaaggt tgttgtccgc acatgctcac ggcacgttcc
     1621 ccagtgttaa cgcttcccag tatacgggaa aaatcgggcg ggacgaatag gaaaatgttg
     1681 gggtttctgg tatcgtaacc cgcacggacc aaacacggga ggctgttttg cccttatacg
     1741 agttttattg tgaaccctgt catacgctgt acacctttcg ttcaccgcgg gtggacaccg
     1801 tcaccctgcc cgtgtgcccg gactgcggac aggagctgaa acgcgaaatc tcgccctttt
     1861 cacacagtgt gcggggaaaa acgtgtgcgg acacggagga ggatccgggg gatctcgatc
     1921 gtatggatgt cgcggccgcg aaactgagcg agcggttgca cgcgctggag cgcgacgatg
     1981 ccgatccacg cgaagccgtg cgggtgatgc gcgaactcgc ccgggaaggc gggatccggt
     2041 tcaaccggga ggttcaggag gccatggcgc ggatcgagtc cggcgaggat ccggaacagg
     2101 ttgaagagac cttccgggag gtgttcgaca cggagaaccc attcgagccg gacaacgaat
     2161 ccccgggccc ggccaccact ggatggtggc gcagaatcca tccgccgcgc cgcgatccgg
     2221 tctggcgcga cctgcccgaa cgcgggtagg tggaaacttc ttgcctgatc ttgttttcgg
     2281 cagcgaaaac ctttattccc gtcagttatc cccgcatcag ccggaggtag gcaccgaaga
     2341 ggacttctcc ccagaaaacc cagatcaggg ctgcggccga gagatagggg ccgaacggga
     2401 tgcggctttg cagtttcacg ccgccgaagg cgatcatgag caggccgcag acactgccga
     2461 gcagggagga gacaaaaacc gtgaacagca ccgcctgcca gccgagaaac gcgccgatcg
     2521 cgcccattag cttgacgtcg ccaaagccca tcgcctcttt cttgaaaatc cggccgccga
     2581 tccacgccac tgcataaagc aacccgaatc ccacggccag accgatgagc gacaacaaga
     2641 ggccctgcca ccacacggtg cgtccgtgca gcgacggcac cagtgcactg aggatcggcc
     2701 ccgccaccat cccgccgatc gtgaccgagt cggggaggat gaagtgctcg aagtcgacga
     2761 aagtgccgag caccagtccg gcgaggaaca gccagtagac cgggatcgcc gcaggatggg
     2821 ggatcgccgt cgtgcccagc gctgccgggg ccgcccactt cagaaagacc gcgagaaaga
     2881 gcgcggcggt gagaagttcc accagcgtgt accggaatgc gattgggcct ttgcatgacg
     2941 cgcaccggcc ccgcagcgcg agccagcttg ccaccgggat gttgagatac cacgggatca
     3001 gcgtgttgca gtgcgggcaa tgcgaacgcg gggtcaccac ggattcgtcg cgcgggatcc
     3061 ggtggatgca gacattcagg aagctgccga cgcaggcacc gaacagtgcg gagaaaaccg
     3121 cgaacaacag aaagaaaaac cggacctcgg tatcattcac aggtacgtga cctcaggttg
     3181 tgggaaccgt gttttctacg gtcgccgatc cgttaccggg ggtgcgcgat tttgaagacc
     3241 caggcgtgat cacagggcgc ggtgccgggg ttgatcgcgg gtgggcggag ggtgatcgtg
     3301 tcgcccgcgc aggtgaaggc gaccggcgcg tcgctgccaa gcagcgtgac cgccgcggca
     3361 ccggacacgg gcgagaccgc gatcggttgc tgcggccagc gcgtgcacag gacgtagagc
     3421 gcgtccggct tccgcgtgaa gtagatgtgg ttcttcccca tgtccttggg gcggtgccgc
     3481 caggtcgtgg tgccgtagat cgcctcgccg ttcacagcca gccatttgcc catgtccagc
     3541 agccgttcct ccatgatcac cgggatcagg ccgtccgcct gcgggccgat gttgagcagc
     3601 aggttcccgc cgcggctgac cttctcgatc agggtttcaa tgcactgcgc ggaggtcatg
     3661 tagtgttcgg tggtctcgaa ccggttgaat ccgaatgatc cgccgatgcc gcggcactcc
     3721 tcccacgggt ggaccaccgg ctgtttctcg ttggcctcat tgctgccgtg gctgtattcg
     3781 gtcgtgtagt ggccgccgtg tttgctgcgg agcttttttc cccagcggtc attgaccacc
     3841 acggtttctt tgactgggga ttcgttgtaa agccaggcca ggaaatcagt gctgcgcaac
     3901 tcgacatcgg taaaatccca ttcgccgtcc gtccagacga tgtccggctt gtagcgcgtg
     3961 accagttcct tcatttgcgg caggttcatc tcggcgaccc agcggccgat ggtctccttg
     4021 gtgtagagcg ggttgcccca ttccagcaaa gagtagtaga accccatgtg gaggcctgcg
     4081 gccttgacgg catcggtcag ttcgcccgcc aaatcgcgat gcggtccgag cacctggctg
     4141 ttccagcgcg ggctgtagtc actcggccag agcgcatatc cgtcatggtg cttcgaggtc
     4201 agcaccacat accgcgcccc ggactttttg aacagagcgg cccactgctc cggccggaag
     4261 tgctcggcac ggaaacgcgt ggcgaaatcg gcgtacgtga cgtgcgggcc gtaaacacgc
     4321 gcgtggaaag aggtgaatgc cggattgttg tgccgcatcc ggttgtcgta gtgttctgag
     4381 tatttggcgt atacgttggc gacgtcggtg ggcgcatacg ccgggaccgc gtacacgccc
     4441 cagtggatga agatgccgaa tttggcatcg caccaccatt gcggcacggg gcgcttgtcg
     4501 atcgactccc agtccgaggt gtagacggtt tgtgcggcga gcgtgccgac gcaggcgaga
     4561 ctggcgagaa gcaggcatct tttcatggga cctccgttac atgacaatca cacatttcgg
     4621 aatatagctt acaggcaaag ggggccgcgg cacaagaccc aatacgcccg caacccaatt
     4681 caatctaaaa ggagcagttg aacgggtgga attctagcag ggctccgtaa acattgggtt
     4741 ttgcggtgcg cccgacgctc tttttgaact cttagtccct gtcgatcgtc gcgggtcgaa
     4801 tgtcgcgggt cgcgggtcga cgatcccgat cccgat
//
LOCUS       DAOSRB010000205         4804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_16090_length_4804_cov_6.321541, whole genome shotgun sequence.
ACCESSION   DAOSRB010000205 DAOSRB010000000
VERSION     DAOSRB010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16090_length_4804_cov_6.321541"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..883
                     /locus_tag="PLZ74_09515"
     CDS             203..883
                     /locus_tag="PLZ74_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98599.1"
                     /translation="MSHSQAAQLPRGLIVEMDFAVLPGHALMQDICRERLAAEGVAFD
                     VKRAAKAFCGRSFTSGLNALCVKAQKTVDAAALAADCNARFEEALSAALSEMPSGFVA
                     FVKAVLAKGVKVVLMTRADVEAVRARFGDSDPEKLIVSHETMPGFCCCGWEGWRRVAR
                     KHDLHERLCVAVAGSGLSVKGALTSGLGVMAKLHPLVEHQDISGSDALITEYSAGLVS
                     EVCRILRI"
     gene            971..1774
                     /gene="mazG"
                     /locus_tag="PLZ74_09520"
     CDS             971..1774
                     /gene="mazG"
                     /locus_tag="PLZ74_09520"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOR98600.1"
                     /translation="MERTFSGIARLDEVMRRLRASDGCPWDREQTLKTLKPCLLEETY
                     ELLEAMDNGDPEHHREELGDVLLQVVFQCAIREEEGCFTFDEVAQVLADKLIRRHPHV
                     FGEVRVDSSSQVLRNWEAIKLTEPDKPANRSALDGVPRSLPALLKAQRMQAKAARVGF
                     DWANAAGAMTKVGEELAELQEAATSGDLRRVTEEMGDLLFSLVNTCRFLDVDAEQALE
                     TANRKFERRFREVERRLRAQGRMLKDCTLEELDAVWEVVKEEETCNDPV"
     gene            1928..2173
                     /locus_tag="PLZ74_09525"
     CDS             1928..2173
                     /locus_tag="PLZ74_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012194023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR98601.1"
                     /translation="MSDEEKNANPQGRPRPVIDADECKSCGRCVNACPRKCLRLAADR
                     LNRRGVKPAEYTGEGCTGCAICFYNCPEPYAIRIEKP"
     gene            2223..3278
                     /gene="vorB"
                     /locus_tag="PLZ74_09530"
     CDS             2223..3278
                     /gene="vorB"
                     /locus_tag="PLZ74_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOR98602.1"
                     /translation="MTRFVKGNEAVVIGALYAGCDVYFGYPITPASEIAHAAAKVFPS
                     VGREFLQAECETASINMVYGAAAAGTLAMTATSGPGMSLMQEGLSYMAGANLPGVVVN
                     VMRAGPGLGNVYPEQGDYNQAVKGGGHGNYACAVVAPASVQEMCDLTMRAFGLAFAYR
                     TPAIVLADGLLGQMMEALVLPERESPRPDTSDWALHGTAATRSHLVTSIYLDAAAQEA
                     HNLRLQAKYAAMRSEARHETYLCDDAELVLLAYGICSRICRTAAEMLRAEGIRAGVFR
                     PITLNPFPAEALCAVLEGRRGMVVELSAGQFRDDVLLHLAKEGGRTQPITLCNRLGGM
                     VMTVEDVVQAARAVAKR"
     gene            3288..4703
                     /locus_tag="PLZ74_09535"
     CDS             3288..4703
                     /locus_tag="PLZ74_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR98603.1"
                     /translation="MTEKKTVGMYETFVRSGKETRSTHYCAGCGHGIIHKLITEALTE
                     LGIQDRTIIVNPIGCAVFGYYYWDCGNVGAAHGRAPSVGTAINRVRRNAVVISYQGDG
                     DLGAIGFNNTFQAASRGEHMAVFFVNNAIYGMTGGQMAPTTLEGMKTATSPFGRDPLT
                     AGYPLHVCEVLDQLKAPVYIERVSVADTKRIMQAKRAVRKALEIQRDGKGYAFVEFLS
                     PCPTNLGMDANAAALFCVEQMEREYPLGCLRDLQATAQPRPAAGAAPSVEAYFGASTQ
                     QSVPEAVIDETFGELRLKFTGYGGQGILSLGVCVAEAARLERRFTTWFPTYGPEQRGG
                     SAACSVVLSGRPIGSPAVDHPDVLVCMNQPSFERFAKDVKPGGTVIVDATVPQQVPVP
                     EGVRVVTMPAIDLAIKMGVPKAANTMMLAALARTGVTRLQDAHLLAALDASFKNKPAL
                     VEKNRLLMREAEAWLDKNAAL"
BASE COUNT          903 a         1309 c         1706 g          886 t
ORIGIN      
        1 acggtgcggc ggggaccgcg acaaaaccca atgtttacgg ggctttgcta gaattctacc
       61 cgttcaacta ctccttttgg gtttagggga cacgcccgga attgctgtgg cgcagaaccg
      121 gcacttgacc gcaagtgagg ggaacgggta cactaaacgg cattttgatg aatcaagagg
      181 caaaaagtga ggagtcgaga agatgagtca ttcccaagcc gctcaattgc cgcgcggtct
      241 gattgtcgaa atggattttg cggtgcttcc cggccatgcg ctgatgcagg atatctgccg
      301 cgagcgattg gccgcagagg gtgtcgcgtt tgatgtcaag cgcgcggcta aagcattctg
      361 cggcagatcg ttcacctccg ggttgaacgc gctgtgcgtc aaggcgcaga aaacagtgga
      421 cgcggcagca ctggcagcag actgcaacgc acggtttgaa gaggcgctgt ccgccgctct
      481 gtcggagatg ccgtccggat tcgtggcgtt cgtcaaggcg gtactggcga aaggggtgaa
      541 ggtggtcctg atgacgcggg ccgacgtcga ggcggtgcgg gcgcggttcg gcgacagtga
      601 tccggagaag ttgatcgttt cgcacgagac gatgccgggc ttctgctgtt gcggatggga
      661 aggctggcgt cgcgtggcgc gcaagcacga tctgcatgag cggctgtgcg tggccgttgc
      721 ggggagcggg ctctcggtca agggcgcgct gacctcggga ttgggcgtaa tggccaaact
      781 ccatccgctg gtcgagcatc aggacatcag cggcagtgat gcgctgatca ccgaatattc
      841 ggccggactg gtctccgagg tctgccggat tctgcgcatc tgaagtgcgt gacagtgcgg
      901 agcgctgtca gatcgagaga gccccgtttg gatgccgaac ggggccatgt cttttttccg
      961 gggaggaagt atggagcgga cattcagcgg gatcgcccga ttagatgagg tgatgcgccg
     1021 gttgcgcgcg tccgacggat gcccgtggga tcgtgagcag acgctgaaga cgctgaagcc
     1081 ctgcctgctt gaagagacct acgaactgct ggaggcgatg gacaacggag atcccgagca
     1141 ccatcgcgag gagttgggcg atgtgctgtt gcaggtggtg ttccagtgcg cgatccggga
     1201 ggaggaggga tgcttcacct tcgacgaggt ggcgcaggtc ttggccgaca aactgatccg
     1261 gcggcacccg catgtgtttg gtgaggtacg ggtcgattcg tccagtcagg tcctgcggaa
     1321 ctgggaggcg atcaagctga ccgagccgga caagccggca aaccgctccg cgctggacgg
     1381 ggtgccccgg agtttaccgg ccctgctcaa ggcgcagcgc atgcaggcca aagccgcgcg
     1441 tgtgggtttt gactgggcaa acgccgcggg cgcgatgacc aaggttgggg aagagctggc
     1501 cgagttgcag gaggccgcca cctccggtga tctgcggcgc gtcacagagg agatgggtga
     1561 tctgctgttc tcgctggtca acacctgtcg ttttttggat gtggacgccg aacaggcgtt
     1621 ggagaccgcg aaccgcaaat ttgaacggcg tttccgcgaa gtcgagcggc gtctccgtgc
     1681 gcaaggccgg atgctcaagg actgcacact ggaggaactt gatgccgtgt gggaggtcgt
     1741 caaggaggag gaaacgtgca acgatccggt ttaggcaagc ggcattccgc agggcgcaag
     1801 ggagtcgcgg atcgttttcg cgcgaccttg cgccagccag acaaacatgg cacactacat
     1861 ttttcgtgcg gaaccggggc ggctgaaccc tcaaggggac gacacgggcg aaggatgagg
     1921 agacgatatg tctgatgagg agaagaacgc caatccgcaa ggcaggccgc gtcccgtaat
     1981 cgacgcggac gagtgcaaaa gttgcggacg gtgtgtgaat gcgtgccccc ggaaatgcct
     2041 gcggcttgcc gcggatcggc tcaaccgtcg cggcgtgaag cctgcggaat acacgggcga
     2101 ggggtgcacg gggtgcgcca tctgttttta caactgtccg gagccgtatg cgatccggat
     2161 cgagaaacct tgaacaacgt gagcggacgg ggctgcgggg cccgtcccgg gagtgaacca
     2221 gcatgacccg atttgtgaaa ggcaacgagg cggtggtgat cggcgccctg tatgccgggt
     2281 gcgatgtcta tttcggctat ccgattacgc cggccagcga gatcgcgcac gccgcggcga
     2341 aggtcttccc ttcggtgggg cgggagtttc tgcaggccga gtgcgagacc gcctcgatca
     2401 acatggtcta cggcgcggcg gcggcgggca cgctggccat gaccgcgaca tcgggtcccg
     2461 gcatgagcct gatgcaggag gggctctcct atatggcggg cgcgaatctt cccggcgtgg
     2521 tcgtcaacgt gatgcgcgct ggcccggggt tgggtaacgt ctatcccgaa cagggggact
     2581 acaatcaggc agtcaaaggc ggcggtcatg gcaattacgc ctgcgcggtg gtggccccgg
     2641 cgagcgtgca ggagatgtgc gacctgacta tgcgcgcgtt tgggttggcc ttcgcctacc
     2701 gtacgccagc gattgtgctg gcggatgggc tgttgggcca aatgatggag gcgctggttc
     2761 tgccggaacg cgaatcaccg cggccggaca cctctgattg ggcgttgcac ggcaccgccg
     2821 cgacgcgttc acatctcgtc acctcgattt atttggacgc cgccgcacag gaggcgcaca
     2881 atctgcgttt gcaggccaaa tatgcggcga tgcgttcgga ggcgcgtcat gaaacctacc
     2941 tgtgcgacga tgccgaactg gtgctgctcg cttacgggat ttgcagccgg atctgtcgga
     3001 ccgccgctga aatgctgcgt gcagaaggaa tccgcgcggg cgtgttccgc cccatcacgc
     3061 tgaacccgtt tccggccgag gcgctttgcg ccgtgctcga aggacgccgg ggcatggtcg
     3121 tcgaactgag cgcgggacag tttcgggatg atgtgctgct gcaccttgcc aaagaaggcg
     3181 ggcgcactca gcccatcacg ctttgtaacc gcttgggcgg catggtgatg acagtggaag
     3241 atgtggtgca agccgcgcgc gcggtcgcga aacgataggg ttggcgcatg acagaaaaga
     3301 agacagtcgg aatgtacgaa acctttgtcc gtagtggcaa agagacgcgg tcgacgcact
     3361 attgcgccgg atgcgggcat gggattatcc acaaactgat caccgaggcg ctgaccgaac
     3421 tcgggattca agaccgcacg atcatcgtga acccgatcgg gtgcgcggtg ttcggatatt
     3481 actactggga ttgcggcaac gtcggggcgg cgcacgggcg cgcgccgtcg gttggcacgg
     3541 cgattaaccg tgtgcggcgc aatgcggtgg tcatctcgta ccaaggggac ggcgatttgg
     3601 gcgccattgg tttcaacaac acgtttcagg cggcgagccg cggcgagcac atggcggtct
     3661 ttttcgtcaa caacgcgatt tacggtatga ccggcggaca gatggcgccg accacgctgg
     3721 aggggatgaa gaccgccacc tcgccgttcg ggcgcgatcc gctgaccgcc gggtatccgc
     3781 tgcacgtctg cgaagtgctc gatcaactga aggccccggt ctacatcgag cgtgtatcgg
     3841 tggcggacac caagcggatc atgcaagcca aacgtgccgt gcgtaaggcg ctggagatcc
     3901 agcgcgacgg gaaggggtac gcgtttgtcg agttcctctc gccgtgcccc accaatctgg
     3961 gcatggatgc gaacgcggcc gcgcttttct gtgtggagca gatggagcgg gaatatccgc
     4021 tgggatgcct gcgcgacctg caggccacgg cgcagccccg cccagccgcg ggcgccgcgc
     4081 cctcggtcga agcctatttc ggcgcgtcca cgcagcagtc ggtgccggag gcggtgatcg
     4141 acgagacatt cggcgagtta cgcctcaagt tcacgggcta cggtggtcaa ggcattctga
     4201 gcctcggtgt ctgtgtggct gaagccgcac ggctcgaacg gcgtttcacg acgtggttcc
     4261 ccacgtacgg gccggaacag cgcggcggtt cggccgcctg ttcagtggtc ctctccggac
     4321 ggccgatcgg gtcacccgca gtagatcatc cggatgtgct ggtgtgcatg aatcaaccct
     4381 cttttgaacg gtttgcgaag gatgtgaaac ccggcgggac ggtgatcgtg gatgcgacgg
     4441 tgccgcagca ggttccggtg ccggaggggg tgcgtgtggt caccatgcct gcgatcgatc
     4501 tggcaatcaa aatgggtgtg cccaaggccg cgaacaccat gatgctcgcg gctctggccc
     4561 ggacgggcgt gacccgtctg caagacgcac atctgttggc ggcgctcgat gcgagtttca
     4621 agaacaagcc ggcgctggtc gagaagaacc gtctgttgat gcgtgaagcc gaggcctggc
     4681 tggataaaaa tgcggcgttg taacggtgcc gcagaatggg gggggtatga taaacgaggg
     4741 gcatgagatc gcggtgccgt tgccggcgcg gacgctgacc gcggcggatg tgccggcgat
     4801 ccgg
//
LOCUS       DAOSRB010000206         4802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_16101_length_4802_cov_6.771645, whole genome shotgun sequence.
ACCESSION   DAOSRB010000206 DAOSRB010000000
VERSION     DAOSRB010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16101_length_4802_cov_6.771645"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLZ74_09540"
     CDS             complement(<1..539)
                     /locus_tag="PLZ74_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOR98604.1"
                     /translation="MSLYLVQGAVYGFAAAVQPGPFLAYLVSLALSRGWRRALPAVLA
                     PLLSDGPIIVLVLIVLSRVPPWWIQLLRFAGGFFILYLAAGALRFWRHPETVAPSDRS
                     DRKGVFRAVLLNLLNPSPYVYWGLVGGPLLLSGWRETPVNGIGFLAGFYGAMVASMAG
                     IILLCACAGKRDERLNRLLS"
     gene            809..2449
                     /locus_tag="PLZ74_09545"
     CDS             809..2449
                     /locus_tag="PLZ74_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98605.1"
                     /translation="MRIGIVWGMVIGWGTVLAAEPCLEYVYPAGVCADSACEVEIGGT
                     HLGNVTQAVISGEGVKAFFLGPVRTVTRNQKGREVPVVLPDRYRFRVVADPEAAPGIR
                     ALRVATAHHLSEPVRFEVGTMSESSEPATLRTETGEVVLSNLPACLNGRCHGRGADVY
                     RFRAAPGSAVVAFVESRTVSANGFVPALTFTDAAGKPCANMKAYDGEDAPVLVLEVRE
                     EGEYRLLVAAGSDAEGGDGCVYRIKLGALPLVTGLTPDRAREGESLNVRLAGHNLARS
                     RLRLFTGGKDSARCLATLTEGALALPSLRFDLANEAQAPGFEATLTPASLNIPPDGSA
                     LVRVEVRRRNGFEGAVRVELDFPPLSIASQGGLIPAGESFCLMTVSTDGARYPRTVFS
                     LPLTATAEINGQPVKRPVVPVRQREGNTAARPQSFDESAARVNPTLYALHLNVAPRKP
                     LSLSTGKPMELMMISSTLAARLGNGCKTVVVWPERGITVQDVQRTNRQERARVMLRTD
                     PSVLQAGASGYLILGCVEKKAPKGPVMAVTQSVPFTVK"
     gene            complement(2436..3278)
                     /gene="mutM"
                     /locus_tag="PLZ74_09550"
     CDS             complement(2436..3278)
                     /gene="mutM"
                     /locus_tag="PLZ74_09550"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOR98606.1"
                     /translation="MPELPEVETVVRELRAQGMVGAVIREVRFRWPRIAAGRSPASLA
                     RALRGRTVTSVTRRAKYIVFTLDTGDRLLIHLRMTGKLRFLGAEERPGRHDHALLLLE
                     DSRRLVFNDTRKFGRLRLCGADTDPFATLGPEPLEPAFTTAVLRARLAGKRGKIKPLL
                     LNQTVVAGLGNIYADESLWHAGIHPERRADSLTETEIQRLCRAIRTILRRAITKGGTT
                     LGGGETNFYSVAGRRGRNADDLRVFRRDGLPCPRCGRPIARSVVGQRGTHFCPVCQRP
                     PTSR"
     gene            complement(3326..4507)
                     /locus_tag="PLZ74_09555"
     CDS             complement(3326..4507)
                     /locus_tag="PLZ74_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOR98607.1"
                     /translation="MKYETTHPWLTFSLRLPPEDYRLWLLLGEVSSKVQHLTGVPLRP
                     DVAADLHRVYLAKGVLATTAIEGNTLSETQVRRLVDGKLDLPKSQQYLQQEVQNILDV
                     CNREARELTRVEGGDRKLCRTLIEQYNRDVLKGLELSEGTVPGKLRPHSVLVGNVYRG
                     APAEDCEFLLEKLCTWLNSDDFSPAEEEFRIPFALIKAIVAHVYLAWIHPFGDGNGRT
                     ARLLEFHILFANGIPLPAAHLLSDHYNLTRVGYYRELARASASGGDLLPFIRYAVRGF
                     LDGIREQIGRIREQQMRVAWENYVYDRFRDAKNSPAQKRRRDLVFALTDRSGWVDVTD
                     IPLLTPKLAREYATAGERMVQRDLNALAKMELISRRHGKARANQHVIEAFLPRRVETS
                     K"
     gene            complement(4572..>4802)
                     /locus_tag="PLZ74_09560"
     CDS             complement(4572..>4802)
                     /locus_tag="PLZ74_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98608.1"
                     /translation="ATVRHKDPVLVAFTLPSDRTHGSPVGVDETQKLHEIRPGASMWV
                     HQPRFYEKSLLVAKVAPKNPERVVKLRHQKFF"
BASE COUNT          908 a         1433 c         1571 g          890 t
ORIGIN      
        1 gacagaagcc ggttcagccg ctcgtccctt ttcccggcgc atgcgcacag caggatgatc
       61 cccgccatac tcgccaccat cgcgccgtaa aaccccgcca gaaaaccgat cccgttgaca
      121 ggcgtttcgc gccaccctga caacagcagc ggcccgccta ctaaccccca atacacatag
      181 ggggatgggt ttagcaggtt caggagcacg gcacggaaca cgcctttccg atcagaacgg
      241 tctgacggcg caaccgtctc agggtgtcgc cagaaacgca acgcccccgc cgcaagatag
      301 aggatgaaga aaccgccagc gaaccgcaac agttgtatcc accacggcgg gacgcggctc
      361 agcacgatca gtaccagcac gatgatcggg ccatcgctca gcagcggtgc cagcacggca
      421 ggcaacgccc gcctccagcc ccgactcaac gccaaagaaa ccagataggc cagaaacggg
      481 ccgggctgca ccgcagccgc aaaaccgtaa accgcgcctt gtacaaggta tagagacata
      541 agcttccttc ttccttggta agtgtatgac tgttatgacg ctgaaacaag cgggtatttg
      601 tctgcacaaa ggacagaaca ccgtgagggg ggctgcggtc ggaaagcggc tacgcgccca
      661 atgattcttg tgcggcggtt ccttgtcagt gtccggcgcg gtgtggtata aggaaaggcc
      721 tttctgctgt gtgcggagcg gaacggaaga gcggtatggc tcgatggggg agtggggcgc
      781 ggcagaggac ggaaggggga ggaacgggat gcggatcgga atcgtgtggg gaatggtgat
      841 cggatggggg acggttctcg cggcggagcc ttgtctggaa tacgtatatc ccgcaggcgt
      901 gtgtgcggat tcggcgtgcg aggtcgaaat cggcgggacg catctgggga atgtgacgca
      961 agcggtgatc agcggtgaag gggtcaaggc gttttttctg ggtccggttc ggacggtgac
     1021 ccggaaccaa aaggggcgcg aggtgcccgt cgtcctgccg gaccggtatc ggttccgcgt
     1081 ggtcgccgat cccgaggccg cgccgggtat ccgcgcgttg cgtgtggcca cggcgcatca
     1141 cctcagcgag cccgtgcggt ttgaggtggg cacgatgtcc gagtcgagcg aaccagcgac
     1201 ccttcgcacc gaaacgggcg aggtcgtgct gtccaatctg ccggcctgtc tgaacggccg
     1261 atgccacggc cggggtgcgg acgtgtaccg ctttcgcgct gccccaggga gcgcggtggt
     1321 cgcgtttgtg gagtcgcgga ccgtgtcggc caacggtttt gtgcccgccc tcacattcac
     1381 ggacgccgcg ggaaaaccgt gcgcaaacat gaaggcgtat gacggggagg atgcgccggt
     1441 gctcgtgctc gaagtgcggg aggagggcga gtacagattg ctggtggcgg cgggatcgga
     1501 tgcggaaggc ggggacgggt gcgtgtaccg gatcaaactc ggcgcgctgc cgctggtcac
     1561 ggggttgacg cctgacaggg cacgggaggg cgagagtctg aatgtgcgtt tggccggaca
     1621 taatctcgcg cggagccgcc tgcgtctttt cacaggcggc aaggacagcg cgcggtgcct
     1681 ggcgacgctc accgagggcg cgttggccct gccttcgttg cgtttcgatc tggccaacga
     1741 agcgcaggct ccgggattcg aggcgaccct gactccggca tcgctgaaca ttccgcctga
     1801 cggcagcgcg ctggtacggg tggaggtgcg gcgtcggaac ggattcgagg gtgcggtgcg
     1861 cgtggaactc gattttccgc cgctcagtat cgcgagccaa ggcggactga ttcccgccgg
     1921 ggagagtttc tgcctgatga ccgtttccac cgacggcgcc cggtatccgc gcacggtctt
     1981 cagtctgccc ctcaccgcca cggcggagat caacgggcag ccggtcaagc ggcctgtcgt
     2041 gccggtgcgg caacgtgagg ggaatacggc cgcgcggccg caatcttttg acgagtcggc
     2101 agccagggtc aatcctaccc tgtatgcgct gcacctgaac gtcgcgcccc gcaaaccgct
     2161 ttccctctcg acggggaagc cgatggaact gatgatgatc agctccacat tggcggcgcg
     2221 cctggggaac ggatgcaaga ccgtggtggt ctggcctgag cgggggatca cggttcagga
     2281 cgtgcagcgg acgaaccgtc aggaacgcgc gcgggtgatg cttcgcacag atccgtccgt
     2341 gctgcaggct ggcgcctctg gatatctcat cctcggttgt gtggaaaaaa aggcgccgaa
     2401 aggccccgtg atggcggtga cgcagtctgt tcccttcacc gtgaagtagg ggggcgctgg
     2461 cagacggggc agaaatgcgt gccacgctgt cccacaacgc ttcgcgcgat cggacggccg
     2521 cagcgcgggc acggcaggcc gtcacgccgg aagacccgca gatcgtcggc gttgcgtccg
     2581 cggcgtcccg ccacactgta aaaattcgtc tcgcccccgc cgagcgtggt gccgcccttc
     2641 gtgatggcgc gccgcaggat cgtgcggatg gcccggcaca accgctggat ctcggtttcc
     2701 gtcagcgagt cggcgcggcg ttccggatgg atcccggcgt gccacaggga ttcgtccgcg
     2761 tagatattgc cgagcccggc cacaacggtc tggttcagga gcaacggctt gattttgccc
     2821 cgtttgccgg caaggcgcgc gcgcagcacg gctgtcgtga aggccggctc cagcggttcc
     2881 ggtccgagcg ttgcaaaggg gtccgtgtct gcaccgcaca accggaggcg cccaaacttg
     2941 cgggtgtcat tgaatacgag tcggcgggag tcttccagaa gcagaagcgc gtggtcatgg
     3001 cggccgggcc gttcctccgc accaaggaaa cgcagtttcc cggtcatgcg caggtggatc
     3061 agcaggcggt cgcccgtgtc gagcgtgaat acgatgtact tggcgcggcg tgtcacgctg
     3121 gtgacggtcc ggccccgcag tgcgcgggcg agcgacgcgg gcgaccggcc ggccgcgatc
     3181 cgcggccagc ggaaccgcac ttcgcggatc acggcaccga ccattccttg ggcacggagt
     3241 tcgcggacaa cggtttcgac ttcaggaagt tctggcatag gcgctcccgt attggggggg
     3301 gttatcccgg tacgtgagga atcgattatt ttgacgtttc cacgcgacgc ggcagaaagg
     3361 cttcgatgac gtgttgattg gctctggctt ttccgtgacg ccgggaaatc agctccattt
     3421 tcgcgagcgc gttaagatcc cgctgcacca tccgctcgcc cgctgtagcg tattcgcgcg
     3481 caagcttcgg tgtgagcaag ggaatgtccg tcacgtcaac ccagccgctt cgatcggtga
     3541 gggcgaagac gagatcccgg cgacgcttct gcgcggggga gttcttcgca tccctgaaac
     3601 ggtcatacac atagttttcc cacgccaccc gcatctgctg ttcgcggatg cgcccgattt
     3661 gttcgcggat gccatcaagg aagccccgca ccgcatagcg gataaagggc aggagatctc
     3721 cgccggacgc gcttgccctt gccagctctc ggtaatagcc gacgcgcgtc aagttgtaat
     3781 ggtcgctcag cagatgggcg gcgggaagcg ggattccgtt ggcgaagagg atatgaaatt
     3841 cgagcaagcg cgccgtccgc ccattgccgt ctccgaaggg gtggatccat gccagataga
     3901 cgtgcgccac aatggccttg atgagtgcga acggaatacg gaactcctct tctgctggcg
     3961 agaaatcatc actgttgagc catgtgcaga gcttttccaa caggaactcg caatcctccg
     4021 caggcgcgcc gcggtagaca ttgccgacca acaccgagtg cggccgcagt tttcccggca
     4081 cggttccttc cgaaagctcc agccctttga gcacatcgcg gttgtactgc tcaatcaacg
     4141 tacggcagag tttccggtcc ccaccctcca cccttgtcag ttcccgtgcc tcacgattac
     4201 aaacatcaag gatgttctgc acctcctgct ggaggtactg ctgggatttc ggcaaatcca
     4261 gtttgccgtc aaccaatcgc cgcacctgtg tttcgctcag ggtgttgccc tcgatagcag
     4321 tggtggccag cacaccctta gccagataga cacggtgcag atcagccgca acgtccggtc
     4381 gcaacggcac cccggttaag tgctgaacct tagacgagac ctcccccagc aagagccaga
     4441 gacgatagtc ttcaggcggg aggcggagtg aaaacgtcag ccacgggtgg gttgtttcat
     4501 acttcatggt ggtcatcata accaattgcc ctgcaggccg tcaacgctac tttgggcttg
     4561 aattgacctt ttcagaaaaa cttttggtgt cgcaatttaa cgaccctttc cgggttcttt
     4621 ggcgccacct tcgcaaccag caaggacttt tcgtaaaaac ggggttgatg cacccacata
     4681 cttgcccccg gcctgatttc atgaagcttc tgtgtttcat cgaccccgac tggactgcca
     4741 tgcgtgcggt cacttggcaa agtaaatgcg accaaaactg ggtctttatg acgcacggtc
     4801 gc
//
LOCUS       DAOSRB010000207         4798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_16113_length_4798_cov_6.460257, whole genome shotgun sequence.
ACCESSION   DAOSRB010000207 DAOSRB010000000
VERSION     DAOSRB010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16113_length_4798_cov_6.460257"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLZ74_09565"
     CDS             complement(<1..242)
                     /locus_tag="PLZ74_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98609.1"
                     /translation="MVSSKSGSGSGFKVSGFLVSGFLVSGFQGFIQIGVGIGIAIGID
                     CFATGHFLGFLVSGFLVSRFQGFIQIGVGIGIAIGI"
     gene            241..3729
                     /locus_tag="PLZ74_09570"
     CDS             241..3729
                     /locus_tag="PLZ74_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98610.1"
                     /translation="MKRKLHEKQTMMSACDCFNAVLRGKETVKPWVARAAAIFVGAAA
                     AMTLDVNAAPMPKVGVTLAFRGGYGSAQYVSNASFKGRPVVEDFAKQGLDLCYYAGWR
                     GAKTPLASLKQFNAVWLVIDHEDQCPYPAEETIAALKEYVSAGGGLVVTHSGGRYSEA
                     PVDAYWTKVYAAFGLDLLHEEIVDDATSARVDRHFDLFRVEDFADHPIAEGIPGLWLP
                     IRRSMGSWGVTAFRTSPEWQWIARTSPTARSVPKGKIDNRPKWDQPGTFAKGPIPVVA
                     ARAFGKGRVVFIAIHKDNVGWAYNIDRWPNWVERSVFNDKRSDALGLLENACAWTAEP
                     SLTDASFTADYKPVEPDYPPYTRHDVGRHENEKPVWKARPVADRKPGVVGLIGLHSSY
                     SDGASTVAEYVAEAKRLGLSFLVFTDPIARLTEDTLGKLRADCAANSSEEFYCCPGVE
                     FREPSGYEWMLFSDKVSWPQESVHRDGRDYVVFDGKNMLQPGNYRGQNLYRGCLLNLG
                     EIPGKGSEYGSLHWMNEVVAVGYGMDADGSRVLYNNAPAYRELPRYLLRCATVSYTRV
                     KRAADLARAKDTSVTCAESLAGVRKMANAQGHGANRAAVAAHVWAAYGTDAEIRDFTH
                     DRILGTDTLRFTVRAVSPTGLKALTVHDATLRVLARFDAKGAKAFEKTFTVTSDRQMY
                     PQLVVEDVKGGKAVSTMREVNYPHAGLHRCGDNFNLLSYNPHVVMHPNWDTQLCPPYK
                     FILMERKHFQISEATFWEAFERAPSREPSVRIQPQWNTLRLENVPFPSEQKHLMASSR
                     TEYTLVAPNVVAIFDQTQGERLVEPTRSDTHGTFSNCPLAIQVGENEWWRHRYRVYQF
                     TDRMDAWWMAVYHQVSPDYRGGYTVVEGALKFLRDATITEPLTLVRLRTHNPKGTVEV
                     FRRNVENFGPGSYHASCGSPAAWHAFFGLKDSVPLVFRESKLPDGVETRVMLTESGRR
                     VKAGEQIRYRFALGSFCEKPHGGMYCDWFASMLDGSGFNHEAVVGRVTDVNGMVEVAA
                     EGNVAKVTFGPTWFIQDYPVRISGLVDNGTAYVMDGAGRLVRPLAFENGRAYAEIPLE
                     KRQTWTFLNLFVADDPALRLSYVPAMPGHEKPTLQILNPTDRDISTRVTDVRTRKTFT
                     VSVPASGCADDVFGAQ"
     gene            complement(3846..>4798)
                     /locus_tag="PLZ74_09575"
     CDS             complement(3846..>4798)
                     /locus_tag="PLZ74_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98611.1"
                     /translation="ASEPRHVFNRRWHMKPGTNPMNPLGGTNDIVRFNPGHLNPNLVK
                     PAGPTDPEICFFALQTPEGKPVALFANYSLHYVGGVRGADISADYFGMFNDRIQQLLN
                     ADRQSPSFISILSNGTSGDINNIDFSKKHEKMPPYAKMRIVAHDVAEKVHAAYRELKW
                     HDHVTVGAAFEEIELQLRKPTPEQVAYAKAFFAEQKENVKPDPWRQSYANRIMQHDRS
                     PATWKFPLQAFRIGDVGISCIPFEVFCEIGLELKQRVPFKPSFTVELANGTYGYLPNP
                     RQHALGGYETWLGSNRVEIEASTKIVDRLLEMFTRLQKGE"
BASE COUNT          915 a         1423 c         1503 g          957 t
ORIGIN      
        1 tcgatcccga tagcgatccc gatcccgacc ccgatttgga tgaaaccctg aaaccttgaa
       61 accagaaacc ctgaaaccag aaacccaaga aagtgtcccg tggcgaaaca atcgatcccg
      121 atagcgatcc cgatcccgac cccgatttgg atgaaaccct gaaaccctga aaccagaaac
      181 cctgaaacca gaaaccctga aaccttgaaa cccgatcccg accccgattt ggatgaaacc
      241 atgaaacgga aacttcatga aaagcaaacg atgatgtcag cgtgtgattg ctttaatgct
      301 gtattgcgtg gtaaagagac ggtgaagccg tgggtcgctc gggctgcggc gatctttgtt
      361 ggggccgctg cagcgatgac gctggacgtg aacgccgcac cgatgccgaa ggttggcgtg
      421 acgctcgcct ttcgcggggg gtacggctcg gcgcagtatg tctcgaacgc gtccttcaag
      481 ggacgccccg ttgtcgagga cttcgcgaag cagggcctcg acctctgcta ctacgctggc
      541 tggcggggtg cgaagacccc gctcgcgtcg ctgaaacagt tcaacgcggt ctggctcgtc
      601 atcgaccacg aagaccagtg cccctatccc gctgaagaga cgatcgccgc gctgaaggag
      661 tatgtctccg ctggcggcgg gcttgttgtc acgcattccg gcggacgcta ttccgaggca
      721 ccggtggatg cgtattggac aaaggtctac gccgcgttcg ggctcgatct tctgcacgag
      781 gaaatcgttg acgatgccac aagcgcacgc gtggaccggc atttcgacct cttccgcgtc
      841 gaggatttcg ccgatcatcc cattgccgag ggcatccccg gactgtggct gccgatccgc
      901 cgcagtatgg ggtcgtgggg cgtgacagcg ttcaggacgt cacccgaatg gcagtggatc
      961 gcgcggacgt cgccgaccgc ccgcagtgtt ccgaaaggca agatcgataa ccgcccgaaa
     1021 tgggatcagc ccggcacttt cgcgaagggg ccgatccctg tcgtcgcggc gcgcgcgttc
     1081 gggaagggcc gtgtcgtgtt catcgccatc cataaggaca acgtcggctg ggcgtacaac
     1141 atcgaccgct ggccgaactg ggtcgagcgc agtgtgttca acgacaagag gtcggatgcg
     1201 ctcgggcttc tcgaaaacgc ctgcgcgtgg acagccgaac cgtcgctcac cgatgcttcc
     1261 ttcacggcgg actacaagcc cgtcgaaccg gattatcccc cgtacacgcg gcacgacgtt
     1321 ggccgccacg aaaatgagaa gcctgtctgg aaggcgcgcc cggttgcgga tcgcaagccc
     1381 ggcgtcgtcg ggctcatcgg gcttcactcg tcctattcgg acggcgcgtc gacggtcgcg
     1441 gagtacgtcg cggaggcgaa acgtctgggg ctttcgttcc ttgtctttac cgatccgatc
     1501 gcccggttga cggaagatac gctcgggaag ctgcgcgcgg attgcgcggc gaactcgagc
     1561 gaggagttct actgttgccc gggcgtcgag ttccgggagc cgtcaggtta tgaatggatg
     1621 cttttcagcg acaaggtttc gtggccgcag gagtccgtcc atcgcgacgg gcgggactat
     1681 gtcgtcttcg atgggaaaaa catgctccag cccggcaact accgcgggca gaacctctac
     1741 cgcggttgcc tcctgaatct gggcgaaatc ccagggaaag gctccgaata cggcagcctc
     1801 cactggatga acgaagtcgt cgccgttggg tacgggatgg atgcggacgg cagccgtgtc
     1861 ctctacaaca atgcccctgc ctatcgcgag ttgccgcgct acctgctgcg ctgtgcgacc
     1921 gtctcctaca cgcgcgtaaa acgggcggcc gatctcgccc gggcgaagga cacgtctgtg
     1981 acctgtgccg aatcgctcgc cggtgtccgg aaaatggcga acgcccaagg tcatggcgcg
     2041 aaccgggccg cagtcgccgc gcacgtctgg gcggcgtacg gtactgacgc ggagatccgc
     2101 gatttcacgc acgaccgcat cctgggcacg gacacgctgc gcttcacggt acgtgccgtc
     2161 tcgccgacgg gactcaaggc gctgaccgtc catgacgcca cgctccgtgt tctcgcgcgc
     2221 ttcgatgcaa agggtgcaaa ggcattcgaa aagaccttta ccgtcacttc ggaccggcag
     2281 atgtatccgc agctcgtcgt cgaggacgtc aaggggggca aggccgtctc cacgatgcgc
     2341 gaggtgaact atccgcacgc gggactccac cgctgcggcg acaacttcaa tctcctctcg
     2401 tacaatcctc acgttgtcat gcacccgaat tgggacaccc aactctgccc gccgtacaag
     2461 ttcatcttga tggagaggaa acattttcag atttcggagg ccaccttctg ggaggcgttc
     2521 gagagggcgc cgagccgcga accctctgtg cgcatccagc ctcagtggaa tacgctgcgg
     2581 ctcgagaacg tgccgttccc gagcgagcag aagcacctga tggcatcgtc gcgcacggag
     2641 tacacgcttg tcgcgccgaa cgttgtcgcg atcttcgacc agacgcaggg cgagcggctc
     2701 gtcgagccga cgcgcagcga tacccacggc acgttcagca actgtccgct cgccattcag
     2761 gtcggtgaga acgagtggtg gcgtcatcgg tatcgcgttt accagttcac agaccgcatg
     2821 gacgcctggt ggatggctgt ctatcatcag gtgtcgcctg actatcgcgg tggctacacg
     2881 gtcgtcgagg gcgcgctcaa gttcctgcgg gacgccacga tcaccgagcc gctgacgctt
     2941 gtgcgcctca ggacccacaa tccaaaggga acggtcgagg tgttccgacg caatgtcgag
     3001 aactttggtc cgggctccta ccatgcctca tgcggcagcc cggcggcctg gcacgcattt
     3061 ttcggactca aggattccgt gccgctcgtt ttccgcgaga gcaagttgcc ggacggcgtc
     3121 gagacgcgag tcatgttaac ggagtcggga cgtcgggtga aggctggcga gcagatacgc
     3181 taccgttttg cgctgggttc gttctgtgaa aaaccgcacg gggggatgta ttgcgactgg
     3241 ttcgcctcca tgctggacgg atcgggcttc aaccacgagg ctgttgtggg ccgcgtgacg
     3301 gacgtgaacg gcatggtcga ggttgcggcc gagggcaatg tcgcgaaggt aacgttcggg
     3361 ccgacttggt tcatacagga ctaccccgtc cgcatttcgg ggctcgtcga caacggtacg
     3421 gcctacgtga tggacggggc cggccgtctg gtccgtccgc ttgcattcga gaatggccgc
     3481 gcctatgccg agattccgct ggagaagcgc caaacttgga cgtttctcaa cctgtttgtc
     3541 gcggacgatc cggctttgcg gctctcctac gtgcccgcga tgccgggaca tgagaagccc
     3601 acgttacaga ttctcaaccc gactgaccgc gatatctcca cacgcgtgac ggacgtgcgg
     3661 acgcgcaaga cgttcacggt atcggttccc gccagcggct gcgcggacga tgttttcggc
     3721 gcacagtagc gcgctcgtgg atcccttgag caaaccgcca agacgccgag gggtgtccct
     3781 gatttccatg aagtttcttg tgtttcaagg ttcaaacgct gctggctcgc agcctgcaac
     3841 ttgcgtcact cccctttttg caggcgtgtg aacatctcca acaggcggtc gacgatcttg
     3901 gttgacgctt cgatctcgac ccggttggag ccgagccagg tctcataacc gcccagcgcg
     3961 tgttgtctgg gattcggcag gtagccataa gtcccgttcg cgagttccac ggtgaacgac
     4021 ggtttgaacg gcacccgctg ctttaactct aagccgatct cgcagaagac ctcgaacggt
     4081 atgcatgaga ttccgacatc gccgatccgg aaggcctgca aggggaactt ccacgtcgcg
     4141 ggagaacggt catgctgcat gatccggttc gcgtaacttt gccgccacgg gtctggtttg
     4201 acattctcct tttgttcggc aaagaaggct ttggcatacg caacttgttc gggcgtgggc
     4261 ttgcgcaatt ggagctcgat ctcctcgaac gcggcgccga cggttacatg gtcgtgccac
     4321 ttgagttcgc ggtaagcggc atgcaccttt tccgccacgt catgagccac gatccgcatt
     4381 tttgcgtacg ggggcatttt ctcatgtttt ttggagaagt cgatgttgtt gatgtcaccg
     4441 ctggtcccgt tactgaggat agagatgaag gagggggact gccggtctgc attaaggagc
     4501 tgctggatgc ggtcgttgaa catcccgaag tagtctgccg agatgtctgc gccgcgcacg
     4561 ccgcccacat agtgcaggga gtagttcgcg aataacgcga ccggcttgcc ttccggggtc
     4621 tgcaaggcga agaagcagat ttcgggatcg gtgggcccgg cgggtttgac caagttcggg
     4681 ttgaggtgtc cggggttgaa acgcacgata tcattcgttc cgccgagcgg gttcattgga
     4741 ttggtgccgg gtttcatgtg ccagcggcgg ttaaagacgt ggcgcggctc gctggcgg
//
LOCUS       DAOSRB010000208         4768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_16236_length_4768_cov_8.266073, whole genome shotgun sequence.
ACCESSION   DAOSRB010000208 DAOSRB010000000
VERSION     DAOSRB010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16236_length_4768_cov_8.266073"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            916..1662
                     /locus_tag="PLZ74_09580"
     CDS             916..1662
                     /locus_tag="PLZ74_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR98612.1"
                     /translation="MTAEEGMALEVRKLGKSFGSGANRVTVLDGVNLNLRRGAFDAVM
                     GPSGSGKSTLLHLLAGLLTPDAGEIRVGGTCLSGMPDSAATCFRRRRIGLIFQDFNLI
                     PTLTAEENILLPLLLDRRAPHTGELAALLERLGLTALRHRVPAKLSGGERQRVAIARA
                     LVTTPDLVLADEPTGNLDSPAGRAFCDLLRDIHTERGCTILLVSHDPVVAAAAERVHL
                     LKDGRVVGELETQHDPARISKRYVEILRQP"
     gene            1817..4648
                     /locus_tag="PLZ74_09585"
     CDS             1817..4648
                     /locus_tag="PLZ74_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOR98613.1"
                     /translation="MLIRLVFKTLRRGKMRFAMATLGVASATGLMVWSLGLTVTAMGQ
                     SRETVRRMTAPFSCWVAAEGAGYRPARGAARPSVPPAALPPQLMESVLSRPEVESAFA
                     CRVIHATLDFRPGGAVLQGPPLVTSLTLAPQEGCPYTEAEVTGDWPDPSSPEPVVAIC
                     SSVFARRHLPPPPCGTPLVLITPHSTVTVRIGAVIDFPQAVDGLPTAFATIGALRQAA
                     GGVWDPRPNLLLCTLRTGAAPDDLRAATGLPPPSTTTGSAYGGTRPGPNAPARFILTD
                     RRQIEARHVSDNLANFKRQAPLLLTLSVLTAFCLLFNALTLGVAQNVRGIALLRAAGM
                     TVGHVAQMVALEGFIMACCGWTLGLLGGWAALTLFTRRAAQTFPAGVMLGWITPAVTA
                     AGIMVLTLLSLLWPCRRAMRIAPLEALAEHDAASQPDRDTRKRSFLRTACGFALLFPM
                     LVFVLPLRLTALSRSVLLLTVGIPLHLFGLFLLLPGFIRATERLCGPVVSALLALDPV
                     LLHRRISRDIPRTAGMTITLAVGLGSFAAIHIWGSSLTRPFLPSPTFPDVIVSLLPNG
                     VSRSAAGAVALLEGVGQQRCLAIEAVQVPLAPALTTRVARVSGRPSPHPNVLLFGADP
                     RTAFGGDRPLAPFRFVEGEPRQAADALTRNDACLITLMFARETGLRKGGTLQLTKRPL
                     PARARSAPSGSRSEEMVSFTVGGVVDLNWHLVTSRAQMRGRHGMPGGTMGPVFVNEEV
                     ARQWSGNTETTCFLWLNFSEAYRAMDPLAAGQRLEADIRKTLAVGSGNTVRVHHRDEI
                     ADGTIAHSADLIGDMARAPFWSLIVLSTGMATLLIASFHASAREIAVLRAIGMTRGQL
                     GRLLLAEALMTSVCGIVLSLVSGLCIGWTFTGWTRAWMPFGGLPVTLEIPWPTLFRGI
                     GFAFALCALMALPPILWLVRRPRFARECN"
BASE COUNT          748 a         1578 c         1474 g          968 t
ORIGIN      
        1 attcgacccg cgacgatcga cagggacgcc cgcgtttccg acaccgcagg tcgctcgtcg
       61 cgggtcgact ttgcacagat tgcgcagatt tttctttttt gtctttgccc gcaagagggg
      121 agggacgagc gtctgctcgt ccgcggcggt cacgccgccg tgccccgcgc tgtgcgcagg
      181 gacacagact tcgtcggata cgcttaacct aaaaagaaag ggttccctca cgatttcgcg
      241 gggaaccctt atgaagcgtc tacgcttatt gtttcggtag tcagaaaaca cttcctttcg
      301 tattgtcttg aatcgactcc ggttcgccct tcaggcgttc cgggggttat gtttgcctct
      361 ttcccctttt caaacgcacc ttggaaagaa tcccgatttt tttccaatca gcgatgatgg
      421 atgcattgcc tctccttcgt actctcaacc gcgttgcaac cgctgtgttt attggcgtaa
      481 gccgccatac ttgttgcccg gtccgtttcc tcggggtggc aagtccctcg tcgtctgtgc
      541 gggtctatca cccaatcaag tcgctttccg cacccgcaga tcttgtcccg caaaaacccg
      601 aacgcgattc tggcaggtgt tttccggcat ccgcaaaaag ggaaactcta aatccgcctc
      661 ctttctcctt attttctcct tatgccccac agaccgatca tgattactat catcccgatc
      721 tttgccgtga ggatacatca aagccgacaa agtgtcaacc cccttttttt aatttttttt
      781 cccggcgcgt aaaccggccc caaaatgaac cgaaagccgt ctgtggccgt tgaacaaaaa
      841 gaaggcgtac ggcgggcggc gcggcaccac ccggcagtcc ctgtgggttc ccgtccgtac
      901 caacaggaga ggagcatgac ggcagaggaa ggcatggcat tggaggtccg gaaactgggc
      961 aagagtttcg gttcgggcgc gaaccgcgtg accgtgctgg acggcgtgaa tctgaacttg
     1021 cggcgcggcg cgttcgacgc cgtgatggga ccaagcggct cgggcaagag taccctgctg
     1081 cacctgctgg cgggattgct caccccggac gccggagaaa tccgtgtggg cggcacctgt
     1141 ctttccggca tgccggactc tgcggccacc tgtttccgcc gccgccgtat cggactgatt
     1201 tttcaggact tcaacctgat ccctaccctg acggcagagg agaacatcct cctgccgttg
     1261 ctgcttgacc gccgggcccc gcacaccgga gaacttgccg ccctgctgga acggctcgga
     1321 ctgaccgccc tgcgccaccg cgtccccgcg aaactcagcg gcggcgaacg gcagcgtgtg
     1381 gccatcgcgc gtgcgttggt gaccacccct gatctcgtgc tggcggacga gccgaccggc
     1441 aatctcgatt ccccggccgg ccgggccttt tgcgatctgc tgcgcgacat ccacacagaa
     1501 cgcggatgca cgatcctgct ggtgagtcat gatcccgtgg tcgccgccgc tgcggaacgg
     1561 gtgcacctgc tgaaggacgg acgtgtggtc ggcgagttgg agacccagca tgacccggcc
     1621 cggatctcca aacggtatgt ggagatcctg cgtcagccct gatgtgtctg cggcggtttc
     1681 tggaaaaccg ccctgccagc gggcgaggac gtccgcgctt ccgacacctc aggccgctag
     1741 tcgcgggtca acgtaaggtc tgacaggcgg agcaagcgtc tgctggcccg tgaagccggt
     1801 acggaaggta aaaaccatgc tgattcgatt ggtattcaaa acgttgagac gcggcaagat
     1861 gcgtttcgcc atggcaaccc tcggtgtggc ctccgccacc ggcctgatgg tttggtcact
     1921 gggcctgacc gtcaccgcca tgggtcagag ccgtgaaacg gtccgccgca tgactgcgcc
     1981 tttctcctgc tgggtcgcgg ccgagggcgc gggataccgc ccggcgcgcg gcgcggcgcg
     2041 gccgtccgtt ccgccggccg cgctgccccc ccaactcatg gaatccgtcc tgtcacggcc
     2101 ggaggtcgaa tccgcatttg cttgccgggt gatccacgcc accctcgatt tccgacccgg
     2161 cggcgcggtg ctgcaaggtc ccccactcgt gacttcgctc acgctcgcgc cgcaggaagg
     2221 ctgcccctac accgaggcgg aggtcaccgg cgactggccg gacccgtcca gtccggaacc
     2281 ggtggtggcc atctgctcat cagtgtttgc ccgtcgtcat cttccgccgc caccgtgtgg
     2341 cacaccgctg gtcctcatca ccccccacag cacggtgacg gtccggatcg gcgcggtgat
     2401 cgatttcccc caagcggttg acgggttgcc gactgcattc gccaccatcg gcgccctgcg
     2461 ccaggccgcg ggcggcgttt gggatccgcg gccgaacctg ctgctctgca ccctgcgcac
     2521 gggtgccgcg cccgacgact tgcgggccgc caccggtctg ccgccaccct ccaccactac
     2581 gggatcggca tacggcggga cccgccccgg accaaacgcg cctgcccgct tcatcctcac
     2641 ggaccgacgg cagatcgagg cgcggcacgt cagcgacaat ctcgcgaact tcaagcgtca
     2701 ggctccgttg cttctgacgc tttccgtgtt gaccgcgttc tgcctgctct tcaacgccct
     2761 gactctcggc gtggcgcaga acgtgagggg gatcgccctt ctgcgcgcgg cgggcatgac
     2821 ggtcgggcac gttgcgcaga tggtggcgct ggaagggttc atcatggcct gctgcggttg
     2881 gacgctcggg ctactgggcg gatgggccgc gctgacgctt ttcacgcggc gggctgcaca
     2941 gacctttccc gcaggggtca tgctgggatg gattacccct gcggtcacgg ccgcgggaat
     3001 catggtgctg acgttactga gcctgctctg gccctgccgt cgcgcgatgc gtatcgcgcc
     3061 gctggaagcg ttggccgaac acgatgccgc cagccagccg gaccgcgaca cgcgcaaacg
     3121 ctccttcttg cgcacggcgt gcgggttcgc gttacttttc cccatgctgg ttttcgtcct
     3181 gcccctgcgg ctgacagccc tttcgcggag tgtgctgctg ttgactgtcg ggatcccgtt
     3241 gcaccttttc ggtctgttcc tgcttctgcc gggcttcatc cgcgccacgg aacgcctgtg
     3301 cggaccggtt gtcagcgcgt tgctggcgct cgatccggtc ctgttgcacc gccggatcag
     3361 ccgtgatatc ccgcgcacgg caggcatgac gatcaccctg gcggtcgggt taggctcctt
     3421 tgccgcgatt catatctggg gcagttcgct gacccgtccc tttcttccca gccccacgtt
     3481 tcctgacgtg atcgtgtcgt tgctgccgaa cggcgtcagc cgcagtgccg cgggcgccgt
     3541 ggctcttctg gaaggcgtcg gccaacaacg ttgcctcgcc atcgaggcgg ttcaggttcc
     3601 gctggctccg gcactcacca cgcgtgtggc gcgcgtctcg ggaagacctt caccccatcc
     3661 gaatgtgctg ctgttcggtg cggatccccg gaccgctttc ggcggcgacc gcccgctggc
     3721 gccgttccgc ttcgtggaag gcgaaccgcg tcaggccgcc gacgccctga cccggaacga
     3781 tgcttgtctc atcaccctga tgttcgcgcg cgaaacgggg ttgcggaaag gcgggaccct
     3841 gcagctcacg aaacgcccgc ttccggcccg cgcccgtagc gccccgtccg gttcgcggtc
     3901 cgaagaaatg gtttcgttca ccgtcggtgg cgtggtggac ctcaattggc atctggtgac
     3961 ctcccgcgcc cagatgcgtg gccgtcacgg catgccgggc ggtacgatgg ggccggtttt
     4021 cgtgaacgag gaggttgcgc ggcaatggag cggcaacacg gaaaccacgt gttttctctg
     4081 gctgaacttc agcgaggcgt accgcgccat ggatccgttg gccgcaggac aacggctgga
     4141 ggccgacatc cgtaagaccc tcgcggttgg cagcggcaac accgtgcgcg tgcatcaccg
     4201 cgacgagatc gccgacggca cgatcgcgca cagcgccgac cttatcgggg acatggcccg
     4261 tgcgccgttc tggtcgctga tcgtgctatc gaccggcatg gccaccctgc tgatcgcctc
     4321 cttccacgcc tccgcacgcg agatcgcggt tctgcgcgcg atcggcatga cgcgcggtca
     4381 gctgggccgt ctgctgctgg ccgaagcgtt gatgaccagc gtgtgcggca ttgtgctgag
     4441 tctggtctcg ggactctgca tcggctggac cttcaccggc tggacccggg cctggatgcc
     4501 gttcggcggc ctgccggtca cgctggagat cccttggccg accctgttcc gcggcatcgg
     4561 attcgccttc gcgctctgcg cgttgatggc gctgccgccg atcctctggt tagtccgccg
     4621 cccgcgcttc gcacgggaat gtaactgagg ataactttga tgtaaggtat aggcaaggag
     4681 ggaggattta gcctgccgcg cggctcagcc gcgaaaaacc ttggaataag tttaggacat
     4741 gattaacacg atgttgttca atcctgaa
//
LOCUS       DAOSRB010000209         4763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_16253_length_4763_cov_7.145072, whole genome shotgun sequence.
ACCESSION   DAOSRB010000209 DAOSRB010000000
VERSION     DAOSRB010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16253_length_4763_cov_7.145072"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLZ74_09590"
     CDS             <1..221
                     /locus_tag="PLZ74_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881273.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOR98614.1"
                     /translation="VQAAIGRTIIAREDIRAFRKDVLAKLYGGDVTRKQKVLKKQKAG
                     KARMKEFGQVNVPQSAFISVLKGSSKDD"
     gene            254..1417
                     /gene="lepB"
                     /locus_tag="PLZ74_09595"
     CDS             254..1417
                     /gene="lepB"
                     /locus_tag="PLZ74_09595"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOR98615.1"
                     /translation="MNYFEKRKIRKQLKTVLHHARTLRCSREDIMRADDLASLNEHIA
                     QAQEAYAARAGEAMKTTGNALETCVTRLNPPRPLSGWRENFDVLVVAISVAMAFRAYF
                     YQPFKIPTGSMQPTLYGIHSESQPPSAATLLDRQPLKFVKWLVTGESFKTVRAKASGT
                     VHFSSSDSSKKPGYMPVIVAGIPHYVPNDAVEVDAYRRPIRLKGGVANGASVRAGEVL
                     WSGVVVSGDFVFVNRWKWNIAHPQRGEVMVFSTIGITALPQGTHYIKRMTGLPNETLQ
                     IKAPNLLVNGKAVTEPLRIGQIARQEKVWPNSPAYAGFNPSGQPQMVLRGRSLATEQD
                     QVTLGPDQYYAMGDNSFNSFDSRYWGPVPERNLLGPATVVYWPFTSPRFGWIR"
     gene            1504..2232
                     /locus_tag="PLZ74_09600"
     CDS             1504..2232
                     /locus_tag="PLZ74_09600"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOR98616.1"
                     /translation="MSANQMTRHQMGLSRRAFVTVAAGAAASLTAAQQGGAAAAAITL
                     PPLPFPSDALAPVVSAQTLSFHHGKHHQGYVNTLNKLLPGSKFAGLTLEAIVRQSAGL
                     AEHAAVFNNAAQIWNHTFYWKSLKPVAVAQTVPAPLAAAVTRCFGSLDACKQAMSDAT
                     LKQFGSGWAWLVAERGALKVVKTPNAETPLTQVGVTPLLTIDVWEHAYYLDWQNRRAD
                     YVKAVLDQALNWPFAAERFAAATA"
     gene            2277..3395
                     /gene="alr"
                     /locus_tag="PLZ74_09605"
     CDS             2277..3395
                     /gene="alr"
                     /locus_tag="PLZ74_09605"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOR98617.1"
                     /translation="MSELPRRVWLEISLETLTRNYRNIAAAVAPAGVLTVLKANAYGL
                     GVVPIAEALCRAGAAGFGVAEPHEALQLLSLGKPVQILSSVLPEEIEPMVEAGVTLPV
                     TDLETARRISAAAAACGRTATVHIKLDTGMGRLGVLAHEAPDVIRAVHALPGLACEGI
                     FSHFPFAYETGSELTNRQMDRFIEIIEELAREGITFDQRHIANSDAINNFPRACRAPF
                     NVVRTGINLHGSFDTVGRRALHLAPVLTLKTRLTAIRTLPAGTGIGYGHTFRLHRSTR
                     VGTVSAGYADGMPLALSNRGYLLIRGTPCPILGRLSMDYTTVSLESVPDARIGDEVVC
                     LGGEGMHALTVEDWATLKNTHPYEIICSFGNRVERRYV"
     gene            complement(3386..4609)
                     /locus_tag="PLZ74_09610"
     CDS             complement(3386..4609)
                     /locus_tag="PLZ74_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOR98618.1"
                     /translation="MNSRREAIFILTRWLATHDFPDRLLPDGPDRAFITDLVYTTVRR
                     RRTLEWVLQQLMRRLPQGETGAALLAGACQILFMPDVAEYAAVNETVEAAKLASKQTA
                     GLVNAVLRNLLRQRETLLARLAKESVGIRTSHPDALITRWRERFGAEETLALCEWNNT
                     PAETFLAYPPGADEPFVPMPRGARVTEMPGFEEGAFIVQDPATAVSVDLLAVRPGLSV
                     LDACAAPGGKTVQIAWRMGVPGPDPRHRLVALDLHEDRLRLVRENLARTRQEWVTVCS
                     GDLTQNPSSALSEFGLFDRILLDVPCSNTGVLRRRPDARWRWTTPRMKSLAATQAVML
                     ENALTLLAPGGRLVYSTCSLEPEENRRQITVLRKRHPEIACIGVEERIPTRHATDGVF
                     ACALERRHDVRGTQT"
BASE COUNT          860 a         1403 c         1598 g          902 t
ORIGIN      
        1 cggtgcaggc cgcgatcggg cgcacgatca ttgcccgcga ggatatccgc gcgttccgca
       61 aggatgtctt ggccaagcta tacggcggcg acgtgacgcg caagcagaag gtgttgaaga
      121 aacagaaggc cggtaaggcg cgtatgaaag agttcgggca ggtcaatgtg ccgcagtccg
      181 cctttatctc cgtgcttaag gggtcatcaa aggacgattg atcgtaaagg cggcggaaga
      241 ggggagtcca agcgtgaatt attttgagaa gcggaagatc agaaagcagc tcaagaccgt
      301 gctgcatcac gcgcgcacgt tgcggtgcag tcgtgaggac atcatgcgtg ccgacgatct
      361 ggcgtcgctg aatgagcata tcgctcaggc gcaggaggcg tacgccgcgc gagcaggcga
      421 ggcgatgaag actacgggca atgcgcttga aacgtgcgtc acgcgcctca acccgcccag
      481 gccgctgtca ggctggcgcg aaaatttcga cgtgttggtg gtggcgattt cggtggcgat
      541 ggccttccgc gcctatttct atcagccctt caaaatccct acgggctcga tgcagccgac
      601 gctgtacggc attcattcgg aatcgcagcc gccctcggct gccacgcttc tggatcggca
      661 gccgctcaag ttcgtgaagt ggcttgtgac cggagaatcg ttcaagacgg ttcgcgccaa
      721 ggcctccgga acggtgcatt tctcctctag cgacagcagc aagaaacccg ggtacatgcc
      781 ggtcatcgtg gccgggatcc cccattatgt gccgaacgat gcggtggagg tggatgccta
      841 ccgccgtccg atccggctca agggcggggt tgccaacggg gccagcgtcc gtgcgggtga
      901 ggttctgtgg tccggcgtgg tcgtgtccgg cgactttgtt tttgtcaacc ggtggaagtg
      961 gaatatcgcg cacccgcagc gtggcgaggt gatggtgttt tccacgatcg gcatcactgc
     1021 cctgccgcag gggacccatt acatcaagcg gatgaccgga ttgcccaatg agacgcttca
     1081 gatcaaggcg cccaatctgc tggtcaacgg caaggcggtg accgaaccgc tgcgcatcgg
     1141 tcagatcgcc cggcaggaga aggtgtggcc gaacagtccg gcctatgcgg ggttcaatcc
     1201 cagcggacag ccccagatgg tcctccgcgg cagatcgctg gctacggagc aggatcaggt
     1261 gacactgggt ccggatcagt attacgccat gggggataat tcgttcaaca gtttcgacag
     1321 ccggtattgg gggcctgtgc ctgagcgcaa tcttctcgga cccgcgaccg tggtgtactg
     1381 gccgttcacc tcgccgcgtt tcggctggat ccgctgaagc gttgcggagt ctgtgcaaag
     1441 aggttacact ggatccatct tttggcgaat gctacacagg ggtgaacccg tgaaaggagc
     1501 gcgatgagtg cgaatcaaat gacgcgacac cagatggggc tttcccgcag agcgtttgta
     1561 accgtggccg ccggggctgc ggccagcctg accgcggcgc agcagggggg tgccgccgca
     1621 gcggcgatca ccctcccgcc gctgccgttc ccttcggacg ctctggcacc ggtggtttcg
     1681 gcccaaacgc tttcgtttca ccacggcaag catcatcagg ggtacgtgaa caccctgaac
     1741 aaactgctgc cgggttccaa attcgccggg ctgacgctgg aggccatcgt gcgtcaatcg
     1801 gctggtttgg cggagcacgc cgcagtgttc aacaacgcgg cccagatctg gaaccatacg
     1861 ttctactgga agtcgctcaa gccggttgcg gtggcacaga cggtgcccgc tcctctggcg
     1921 gctgcggtca cccgctgctt tggttcgctc gatgcctgca aacaggcgat gtcggatgcg
     1981 accctgaagc agttcggcag cgggtgggcc tggctggtgg cggaacgggg ggcattgaag
     2041 gtggttaaga cgccgaacgc ggagaccccg ctgacgcagg tcggcgtgac gccgctgctg
     2101 acgatcgatg tttgggagca cgcctattat ctcgattggc agaatcgccg ggcggattac
     2161 gtcaaggctg tgctcgatca ggcgttgaac tggccgttcg ccgcggagcg tttcgcggcc
     2221 gcgaccgcat gagggttgcc gcgcgcgtgg tgagagcaag tcggtaaggg ccggagatga
     2281 gtgagttgcc ccgcagagtc tggctggaga tcagcctcga aacgctgacg cgtaattacc
     2341 ggaacatcgc ggcggcggtc gcgcctgccg gggtgttgac ggtactcaag gccaacgcct
     2401 atggccttgg ggtagtcccg atcgccgagg cgctctgccg cgccggtgcg gcggggttcg
     2461 gcgtggccga accccacgag gcgctgcaac tgctttcgtt gggcaaaccg gttcaaattc
     2521 tgagcagcgt gctgcccgaa gagatcgaac ccatggtaga ggccggggtc acactgccgg
     2581 tcacggatct cgaaaccgcg cggcggatca gcgccgctgc tgcggcgtgc ggccggaccg
     2641 ccacggtgca catcaagctc gacaccggta tggggaggct gggggttctg gcgcacgagg
     2701 ctccggacgt catccgtgct gttcatgcgc tgcccgggtt ggcgtgcgag gggatctttt
     2761 cgcactttcc cttcgcgtac gaaaccggat cggaactgac caaccggcag atggatcgtt
     2821 ttatcgaaat catcgaggaa ctcgcgcggg aagggatcac ctttgaccag cgtcatatcg
     2881 ccaacagcga tgcgatcaac aacttcccgc gcgcctgccg cgcgccgttt aatgtcgtgc
     2941 gcaccggcat caacctgcat ggatcgttcg acaccgtcgg acggcgtgcc ctgcatctcg
     3001 cgcccgtgct gacgctgaaa acgcggctca ccgcgatccg gacgctgccc gccggtacgg
     3061 gtatcgggta cggccacacc ttccgcctgc accgttcaac ccgtgtgggc acggtctccg
     3121 caggttatgc ggacggcatg ccgctggccc tgtccaaccg cgggtacctg ctgatccgcg
     3181 ggacgccctg tccgattctg ggaaggctct ccatggacta caccaccgtc tcactggaaa
     3241 gtgtccccga cgccaggatt ggcgatgagg tggtctgtct gggcggcgaa ggcatgcatg
     3301 ccctgacggt cgaggattgg gccacgctca aaaacacgca cccgtacgag atcatctgtt
     3361 ccttcggcaa ccgcgtcgaa cggcgttacg tttgagtccc gcgcacgtca tgccgacgtt
     3421 cgagggcgca ggcaaagacg ccgtctgtgg cgtggcgggt cgggatgcgt tcctccacgc
     3481 cgatgcaggc gatttccgga tggcgtttcc gcaggaccgt gatttgacgg cggttctcct
     3541 ccggctccaa gctgcatgtt gaatagacga gcctgcctcc gggcgcgagt agcgtcaggg
     3601 cgttttccag catgaccgcc tgtgtggcgg ccagactttt catgcgcggg gtggtccagc
     3661 gccagcgggc gtcaggtcgg cggcgcagca caccggtgtt cgagcagggc acgtcgagca
     3721 ggatgcggtc gaacaagccg aactccgaca gggcggagga cgggttttgc gtcaggtcgc
     3781 ctgagcacac cgtgacccat tcctgacggg tgcgggccaa attttcgcga accagccgga
     3841 gccggtcctc atgcagatcg agcgccacga gccggtgcct ggggtccggg ccgggcacgc
     3901 ccatgcgcca ggcgatctgc acggtcttgc ctccgggagc cgcgcaggcg tccagcacgc
     3961 tgagacccgg acggacagcc agcagatcga cgctgaccgc tgtggccgga tcctgcacaa
     4021 tgaacgcgcc ctcttcaaat cccggcatct ctgtgacacg cgcgccgcgc ggcatgggca
     4081 cgaacggttc gtccgcgccg gggggatagg cgagaaaggt ttcggcaggg gtgttgttcc
     4141 attcgcagag cgcgagcgtc tcttctgcgc cgaaccgttc gcgccagcgg gtgatcaggg
     4201 catcgggatg ggaggtgcgg atgccgaccg actctttggc caaccgggcc aacaaggttt
     4261 cgcgctggcg caacaggttg cggagcaccg cattgacaag gcctgcggtt tgctttgaag
     4321 cgagttttgc ggcctccacc gtttcgttga ccgccgcgta ttcggcgaca tcgggcatga
     4381 agaggatttg gcacgcgccc gccaacaggg ctgctccggt ttcgccttgg ggcaggcgcc
     4441 gcatgagttg ctggagtacc cactccagtg tgcggcggcg tcggactgtg gtgtaaacga
     4501 ggtcggtgat gaaagcgcgg tcgggtccgt ctggaaggag tcgatcagga aagtcgtgcg
     4561 tggcaagcca acgggtaagg atgaagatcg cttcgcgcct ggagttcatg cggtgtgttt
     4621 ttctgtggga tcggttatgg tcatcttccg gcacgttgct ccgggtaaga tgaagaacta
     4681 accttatatc cgcccaagcg gtttgtcgat ctgaaaacgt tatggtcaag tctcaacgtc
     4741 attcctgccg ctgattcgaa ccc
//
LOCUS       DAOSRB010000210         4716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_16460_length_4716_cov_7.673032, whole genome shotgun sequence.
ACCESSION   DAOSRB010000210 DAOSRB010000000
VERSION     DAOSRB010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16460_length_4716_cov_7.673032"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..940)
                     /locus_tag="PLZ74_09615"
     CDS             complement(170..940)
                     /locus_tag="PLZ74_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR98619.1"
                     /translation="MEKSPPLPLAENTALITGASSGIGRAIAETLARLGAAVVIHARR
                     RERLEDLAASIVAKGGRALVVAGDAGETADIDCLLDAAQNWEEGGRKLDIVVANAGRG
                     LAGGVTNSDLSQWRELYQTNVLGTAYLLRRAGLLFAAQGHGDIVAVGSVVGRNISPFS
                     GFYGSSKFALGALTEALRREVCARGVRVCTVLPGIVESGFQQVAGYTPENFGRSIAKF
                     GRVLDPQAIADGVAWVLTQPPHVNVSDITIRPIGQDYP"
     gene            complement(1063..4500)
                     /locus_tag="PLZ74_09620"
     CDS             complement(1063..4500)
                     /locus_tag="PLZ74_09620"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOR98620.1"
                     /translation="MLATMLAAVMEVTAPQIDGHLDDACWAKAEWITDFQLFKNVVDD
                     GQPRKATAFAIVQDGTSLYVGVKCAEPDMQALNARPDTGLWSECVEIYLSPGKTTFEF
                     YQFAIGPATKDVFAEFYSEGGNIRPDSYRPVWRHAFAKDEKGWTAEIAIPLSSLYMSR
                     NKDWKSEWLVNFIRTTKTGKTPAADYTWSPLKLHVRESENFNMLSGFPKRAAEEDCAV
                     LAVIANITGREGGQLKGTLNVDVFAGLAGRYHVVTSSGADTELDLKAGNNKIVLPCVY
                     AENGRNWTHFKVQKVGFARACERDFPVMVDFRPIRVKLTQPGYRNNFYPGQKTDRIAG
                     RVTVGSGKEAKLTLTGEGFTTRTLTFTDGSGDFDFDTTGFKVGTDAFLTVVADGAEEK
                     VRIRNLAPTGRRMTWIENGNFVVNGKPVIRRTLDAMNWHQGKANWEKFQKERASFRLT
                     EDLQNVGIEAMRMINGIEQREGIYDRRPSDAVFAKMDEKLAQVKDTDYACWFIIDEPE
                     CRNISPVWLKHQYDYLAEKDPYHVITTDSRGGKTYIDCFDVVQTHPYMAPVNCEDGVR
                     RYGVQACDIGSYLDAFEAADRPDKCIGVVPQAFAYRWYSTRNDYLTLEEYLATTWAAI
                     LRGAKSVRPYACHDMGDRAQLWEGTRYIFESIEALEDYLLFAKRETLTKSKLTETTLY
                     TLANGEKMVVAVNFTPEKQTASFPGVKGVLREFRGERIFNLQSSAVNLDPFEVVIATT
                     DERGKGLESFKAMAQRVAEGETERLSRDNQILEKHGDVVITSNMDGSSGKFYKLIDGC
                     RDMWAAYSVWKDAPFVEMSFPHFTPVFEKLRVYGYGLDQMKVENRCAGEWQTLEPKSV
                     KTEKWLRELDFGEPVRTVALRFSFPAPKRTRNEIELYEIELPQAQSAAAQTVAVKVKP
                     LEDEGVLAVYGKDSHISQNVTWELPNEARPKWAVLDFAAFNPKSPRQYMAWHVRLETP
                     EAGGNQLLASLAGQPEEAGVYTIRFDPPLEPMRKWITLRDYGLEIDFGGLTLLDKPAN
                     RVDLRVPEDAQDVSIGDTIDVKVDFAEPCEDVSAEFLYAFKTLEALNPYAINGKSGLQ
                     LKRLDDLGRSWGTAVKVLELPRDVPARCVYVKAHALGGGFKRPLYGNFAVPFVRSRN"
BASE COUNT          926 a         1451 c         1329 g         1010 t
ORIGIN      
        1 gcgtcccgca tacgcaccgg tctccaaaac tcagtcgatt gcgcttgcga cgaaggggtg
       61 cgatgaagtg ctgggacgaa gagatcaaaa agagcgtcgg gcgcaccgca aaacccaatg
      121 tttacggggc tttgctagaa ttctacccgt tcaacggctc tttttgcggt caggggtagt
      181 cctggccgat ggggcggatc gtgatgtcac tgacgttgac gtggggtggt tgggtcagga
      241 cccaagccac gccatcggcg atggcctgcg ggtcgagcac gcgtccgaac ttggcgatgc
      301 tacggccgaa attttcgggc gtataccctg cgacctgctg gaatccgctc tccacaatgc
      361 cgggcaggac ggtacagacg cgcacgcccc gcgcacagac ctctcgtcgc agcgcctccg
      421 tcagtgcgcc gagcgcgaat ttgctggatc cgtagaagcc gctgaacggc gagatgttgc
      481 ggcccaccac cgaaccgacg gccacgatgt cgccgtgtcc ctgcgcggcg aagagcagtc
      541 ccgcgcggcg caggagatac gcggtgccca gcacgttggt ctggtacaat tcgcgccact
      601 gcgacaaatc actattcgtc acgccgccgg ccaagccgcg acccgcatta gccacgacga
      661 tatcgagttt gcggccgccc tcctcccagt tttgcgccgc gtccaatagg cagtcgatgt
      721 cggccgtctc gcccgcatcg cccgccacaa cgagcgcgcg tccgcctttc gcaacaatcg
      781 acgcggccaa atcctccagc cgttcgcgac ggcgtgcgtg gatcaccacc gcggcaccga
      841 ggcgtgcgag ggtttcggcg atggcgcggc cgattccgga actcgcgcca gtaatcaagg
      901 cggtgttttc ggcaaggggc aggggagggg acttttccat gtttctctcc ttcaggggtt
      961 cctaagcttt tttcagaata gcatcggtcc tgccgtaccc gcaagtcgtc cgtacccaat
     1021 cggttgatgg cgggggtgat taaaacaaat tgaactttcg gatcaattgc gcgatcggac
     1081 gaacgggaca gcaaagttgc catagagcgg acgtttgaag ccgccgccga gggcatgtgc
     1141 cttgacatag acgcagcgcg ccgggacgtc ccgtggtaat tccaaaacct tgactgcggt
     1201 gccccaactg cgtccgaggt catcaagcct cttgagttgt aacccgctct taccgttgat
     1261 agcgtaaggg ttgagggcct ccagcgtctt gaatgcgtag aggaactcgg ccgagacatc
     1321 ctcacaaggc tcggcgaaat cgaccttcac atctatcgta tcgccaatcg agacgtcctg
     1381 cgcatcttct ggtacgcgca gatcaacgcg gttggccggc ttatcgagga gcgtcaggcc
     1441 gccgaagtcg atctcgagtc cgtagtcgcg cagcgtgatc cacttgcgca tcggttccaa
     1501 tggtgggtca aaacggatcg tatagacacc cgcttcctcc ggttgacccg caagcgaagc
     1561 gagaagctga tttccccccg cctccggagt ttccaaacgc acatgccagg ccatgtactg
     1621 ccgcggcgat ttcggattga aagcggcgaa atcaaggacc gcccacttcg gtcgggcttc
     1681 gtttggcagt tcccaagtta cgttctgcga aatatgcgag tccttgccgt aaacggcaag
     1741 gacgccctcg tcttcaagcg gtttgacctt cacggccacc gtttgcgcag ccgccgactg
     1801 cgcctgaggc aactcaatct cgtacagttc gatttcattg cgcgtgcgct tcggagccgg
     1861 gaaggagaag cgcaacgcga ccgtccggac aggctcgccg aaatcgagtt cgcgcagcca
     1921 cttctcggtc ttcaccgatt tgggttccag cgtctgccac tcgcctgcac agcggttctc
     1981 gaccttcatc tgatcaagtc cgtaaccgta tacgcggagc ttctcgaata ccggtgtgaa
     2041 gtgcgggaac gacatctcga cgaaaggagc gtccttccac accgaatacg ccgcccacat
     2101 gtcgcggcag ccgtctatca acttatagaa cttgccgctc gaaccatcca tgttggaggt
     2161 gatcaccaca tcaccgtgct tctccaggat ctggttgtcg cgagagagcc gttcggtttc
     2221 cccttccgcc acacgttgcg ccatcgcctt gaaactctcc agtccctttc cgcgttcgtc
     2281 cgtcgttgcg atcacaactt cgaatgggtc gaggttgacg gctgaagatt gaagattgaa
     2341 aatacgttcg ccacgaaact cgcgcaacac gcctttgaca cccggaaatg acgcagtctg
     2401 tttttccggc gtaaagttga ccgcaacaac catcttctcg ccgttcgcaa gcgtgtagag
     2461 cgttgtctcc gtgagcttgc tcttcgtcag cgtctcgcgc ttggcaaaaa ggaggtagtc
     2521 ctcgagcgcc tcgatggact cgaaaatgta gcgcgtcccc tcccagagct gcgcacggtc
     2581 ccccatgtcg tgacaagcgt agggacggac agatttcgcg ccgcggagaa tcgctgccca
     2641 ggtcgtggcg aggtactctt cgagcgtcag atagtcgttg cgcgtcgagt accagcggta
     2701 ggcaaacgcc tgcggaacga ccccgatgca cttgtcagga cgatctgccg cctcgaaggc
     2761 gtctaaataa gagccgatgt cgcacgcctg cacgccgtaa cgccggacgc cgtcttcgca
     2821 gttgacgggt gccatgtagg gatgcgtctg gacaacgtca aagcagtcaa tatacgtttt
     2881 gccgccacgt gaatccgtcg tgattacgtg atacggatcc ttctctgcca gatagtcgta
     2941 ctggtgcttg agccacacgg gtgagatatt acggcactcc ggttcgtcaa tgatgaacca
     3001 gcatgcgtaa tccgtatcct tgacctgtgc gagcttttcg tccatcttcg cgaagaccgc
     3061 gtcagaggga cggcgatcgt agatgccttc gcgctgctcg atgccattga tcatgcgcat
     3121 cgcttcgatg cctacgtttt gtagatcttc agtcaggcgg aagctggcac gttccttctg
     3181 aaacttctcc cagttggcct tgccctgatg ccagttcatc gcatcaagcg tacggcggat
     3241 cacgggcttg ccgttgacga cgaagttccc attctcgatc caggtcatcc tccggcccgt
     3301 cggcgccaag ttgcggatcc tgaccttctc ctccgcacca tcggccacga ccgtcaggaa
     3361 ggcatcggtt ccgaccttga agccggtcgt gtcgaaatcg aagtcgcccg agccgtccgt
     3421 gaacgtgagc gtgcgcgtcg tgaacccctc gcccgtgagc gttagcttgg cctccttgcc
     3481 ggaaccgacc gtgactcgtc ccgcgatgcg gtccgtcttc tgacccggat aaaagttgtt
     3541 gcggtagccg ggctgcgtga gcttgacacg aatgggacgg aaatcaacca tcaccggaaa
     3601 atcgcgctcg caagccctcg cgaaaccgac cttctgcact ttgaaatgcg tccagtttcg
     3661 gccgttctcc gcataaacac acggcaggac gattttgttg ttccctgcct tcaggtctag
     3721 ctcggtgtca gcaccgctcg atgtcacgac atgataacgt cccgcgagtc ccgcaaagac
     3781 atcaacgttg agtgtcccct tgagttggcc tccctcgcgt cccgtgatgt tggcgataac
     3841 cgccagcacc gcacagtctt cctcggccgc gcgtttcggg aatccgctga gcatgttgaa
     3901 gttctcgctt tctcttacat ggagcttgag cggactccac gtgtagtccg ccgcaggcgt
     3961 cttgccggtc ttcgtggtgc ggatgaagtt gacgagccac tcgcttttcc agtccttgtt
     4021 gcgcgacatg tagagagacg ataacgggat cgcaatctcg gccgtccatc ccttctcatc
     4081 cttggcgaac gcgtgcctcc agacaggacg atatgagtcg ggacggatgt tgccgccttc
     4141 cgaatagaac tcggcaaaga catcctttgt cgccggcccg atcgcgaact ggtagaactc
     4201 gaatgtcgtc ttgccgggcg agagatagat ttcaacgcat tcggaccaga gtcctgtgtc
     4261 aggacgcgcg ttgagcgcct gcatgtccgg ctcggcgcac ttgacgccga catagaggct
     4321 cgtgccgtcc tgcacgatcg caaacgccgt cgccttacgc ggctgtccgt cgtccacgac
     4381 gtttttgaaa agctggaaat cggttatcca ttcggctttc gcccagcacg cgtcatcgag
     4441 atgcccgtca atctgcggcg cggtaacttc catgactgcc gccaacattg tcgccagcat
     4501 catcatcttc tccccaaaaa aacaagttgc ttctcacaca aaaccactaa ggtccttcgg
     4561 tctgcgcggc tacgggtgcg aaacggcctg cgcggcgcgt gatcatcgtc cgggtgccag
     4621 cgatttccac gtcgaaaatc gttgcatcgg attttgcatc atagtactgc acaagcttta
     4681 cggtgaacgg cttgtcgagc ccgcggatct tgccga
//
LOCUS       DAOSRB010000211         4640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_16817_length_4640_cov_6.797165, whole genome shotgun sequence.
ACCESSION   DAOSRB010000211 DAOSRB010000000
VERSION     DAOSRB010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16817_length_4640_cov_6.797165"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..2136
                     /locus_tag="PLZ74_09625"
     CDS             145..2136
                     /locus_tag="PLZ74_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98621.1"
                     /translation="MTMHETNDLRLLAEEENGMSARPGRPSLLHKTGKLLFCGLTAMG
                     LAGALSARGDLYVNIATGNDGNDNPNSAERPYKTLQRAITRLNELKVDGVVIHVAPGT
                     YAPANSTFHGHEFPRLNFSVVGEGTAENPVVIDGGGSNACFRITNSKKIGAFSNIIFR
                     NGLHYSGGGIGCLAGWTTSGVSGVTNCVFENCHSTNGCGGAVMSYGYTTSFSNCTFRN
                     CSAVRVDNGSWSGQVKNGCGGAVMLYAVKGDCDFTACSFETNAASGGGGAVMMQVDDA
                     PGGTMEQSDIGLRLTDCTFVGNTSGGQGIVNGIVRSCERTAFTGNSASWGSVWFLDGY
                     LGTKYGGAYFVTNRFVNCTFTDNLGTNVGCFGWSYSKRFEFEHCTFAGNTSLNGAAVL
                     QTDGHSPHCFFRDCTFSRNAGEGVLAVTSHNGANFTLELARTVFTENDAGAKPLFQNL
                     SASRILNSCFSANCGGSVLDFASGGGGVYHTNTLRNCLLDHNTNTVNEGILRIFWTLA
                     ETADVCNNTFVDNVATDQPSALWINSVASYEDLGCHYANNLFFANRKTDGTSVKQTAG
                     VFNSFATNNWIEVGGTTLADGTRGNIVGVDPKFTNRADGDYTISAASACRDCGAFFEW
                     MTGACDIRANPKYPRVTGAAPDIGCYEWLPERYLTVIVR"
     gene            2154..4454
                     /locus_tag="PLZ74_09630"
     CDS             2154..4454
                     /locus_tag="PLZ74_09630"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOR98622.1"
                     /translation="MDWFLLFLALAVPVVAVGETRFNGMKYFGAPGDWISVRLDGFTT
                     EELPALFENDGVRQVIVRHEVPNIAGVHGPKGFPFPNWGDLTVTLRSWDGAFSRVLGT
                     QKTGCWGFGQERRFPLSFKGLKPGAYLVVAEMPPHIRAEGVFNPTNTGSGAIGLPMLY
                     GRNAMRFAIYPKTDPHRVFGVGNRMIHTGKWWTGYQLANTLEARDLQPVCVSDGDSFQ
                     NAILGAYRVEHTTVDSAGPTNVPHLNNPVARMLDIFSPTGRVELVRRGEETGRRLAKD
                     AGVLALALGGEKPSFNAGRVCPTEWADADFRAFCRARYTNDLAAANRVWGRTFTDWRE
                     VRQPIYGVQSAATEKTGAAAIDWYANMGNMSDEFVKEINRPENVEFAMDWYRWITKAS
                     IEMYATYADAARRHDRKTLYTNNYCWPNFFAHLCLPTWRRLDAAALDFQYVCTFPRTL
                     GSNSEMIEIAEMTESAMGNKPIYGFEVYVQPHYPAEMAALQNWAMVAHGMRVNLVFAW
                     KPYSDYGGKVFENGPKAWTRPADAKPMWMIIDTDGTRLPCYAAVKRSSEEIAAWHSQF
                     DALSVRRTRGRTAVYLSDETGMYTMLRTGNRPYLENHLCHARNRIASGLRLGGVRIEY
                     LDDETLEKLVRRDFDAVVLPPTPRIAPESARRLDAFERAGGEVIRLDDWSADFFDRYP
                     QIPRSAYWRAPDGRVNDVVVVVRTQEGTGRRFVFVLNRTGEAVEGELAGRDFTEGQTF
                     ADTLRGGKVGRSFRLDGWGYRVLLCD"
BASE COUNT          880 a         1375 c         1487 g          898 t
ORIGIN      
        1 acgcccgcgc tcccgactgc gcacacacaa accacgcact aaactcgcat gcccctgtca
       61 cgaaccgatt acagagcaag cccaattgac gcaacgggtc aggggtgata ttgtaaatcg
      121 taagtccaac aaaggggaac acacatgact atgcacgaaa ccaatgacct gcggctgctg
      181 gctgaagagg aaaacggcat gtcagcgagg ccggggcgcc cgtccctgct ccacaagacc
      241 ggaaaacttc tgttctgcgg attgacggcg atggggttgg ccggtgcgtt atccgcacgc
      301 ggcgatctct acgtgaacat cgcgacgggc aacgacggga acgacaaccc gaattctgcg
      361 gagaggccgt acaagacact gcaacgggcc atcacgcgcc tgaacgagct caaggtggac
      421 ggtgtcgtga tccacgtcgc gcccggcacg tatgcgccgg caaactcgac cttccatggc
      481 catgaatttc cgcggctcaa tttctccgtt gtcggcgaag ggaccgcgga gaaccccgtc
      541 gtgattgacg gcgggggttc gaatgcctgt ttccgcatca cgaattcgaa aaagatcggc
      601 gcgttctcca acattatttt tcgaaacggt ctccactatt ccggcggcgg tatcggctgt
      661 ctggcgggct ggacgacgtc cggtgtctca ggggtgacga actgcgtgtt cgagaactgc
      721 cactcgacga acggctgcgg cggcgcggtg atgtcgtacg gctatacgac gtccttttca
      781 aattgcacgt tccgtaattg ctcggcagta cgtgtggata acgggtcgtg gtccggacag
      841 gttaagaacg gttgcggcgg tgcggtgatg ctctacgccg tgaagggtga ttgtgacttc
      901 acggcgtgtt cattcgagac gaatgcggcg tccgggggcg gtggagcggt gatgatgcag
      961 gtggatgacg cgcccggcgg gacgatggag cagtccgaca tcggcctgcg tctgacggac
     1021 tgcacctttg tcgggaacac gtcgggcgga caaggcatcg tgaacggcat cgtccgttcg
     1081 tgcgagcgga cggccttcac cggcaactcg gcctcctggg gcagcgtttg gtttttagat
     1141 ggttatctgg ggacgaagta cggtggcgcc tactttgtca cgaatcgctt cgtgaactgc
     1201 acattcacgg acaatctcgg taccaacgtc ggttgcttcg ggtggagtta ctcgaaacgt
     1261 tttgaattcg agcactgtac gttcgctggc aacacctcat tgaacggcgc cgccgtgctc
     1321 cagactgatg gacattcgcc tcactgtttc ttccgggact gtaccttcag ccgcaacgcc
     1381 ggcgagggcg tcttagcggt cacgtcccac aacggggcga acttcaccct cgagctcgcg
     1441 cggaccgtgt tcacggaaaa cgacgccggg gcgaagccgc tgttccagaa tttgtcggcc
     1501 agccgcatcc tgaactcctg tttctccgcc aactgtggag gcagcgtact tgatttcgcg
     1561 tccggaggcg gcggggtcta ccacacgaac acattgcgga actgtcttct cgaccacaac
     1621 acgaacaccg tcaacgaggg catactgcgg attttctgga cgcttgcgga gacagcggac
     1681 gtctgcaaca acacgttcgt ggacaatgtc gcgacggacc agccctccgc tctctggatc
     1741 aacagcgtag cctcctacga ggatcttggc tgccactacg cgaacaacct cttcttcgcg
     1801 aaccgcaaga cggacggcac gtccgtgaaa cagacggccg gcgtcttcaa ctcctttgcg
     1861 acgaacaact ggatcgaggt gggcggcacg acgctcgcag acggcacgcg gggtaacatc
     1921 gttggcgtgg acccgaaatt cacgaaccgc gcggatggcg actacacgat cagcgcggcg
     1981 tcggcctgcc gggactgcgg cgcctttttc gagtggatga cgggtgcatg cgacatccgg
     2041 gcaaacccga agtacccgcg cgtgacgggc gcggcgcccg atatcgggtg ctatgagtgg
     2101 ctgcccgagc gttatctcac ggtgattgtc cgttgaacgt ccggaggatc gggatggact
     2161 ggtttcttct tttcctagcc ctcgccgtgc cggtcgtcgc ggtcggcgag acgcgcttca
     2221 acggcatgaa gtacttcggc gcgccagggg actggatttc cgtacggttg gatggcttca
     2281 cgaccgagga actgcccgcc ctttttgaaa acgatggcgt gcggcaggtg atcgtgcggc
     2341 acgaggtccc gaatatcgcg ggcgtgcatg gcccgaaagg tttcccgttc ccgaactggg
     2401 gcgatctcac cgtgacactg cggagttggg acggggcgtt ctcccgcgtc cttggaacac
     2461 agaagaccgg ctgctggggc ttcggccagg agcgtcgctt tccgctctcc ttcaagggcc
     2521 tgaagccggg cgcttacctc gttgtcgcgg agatgccgcc gcatatccgc gcggagggag
     2581 tcttcaaccc cacgaatacc ggtagcgggg cgatcggcct gccgatgctt tacggacgca
     2641 acgcgatgcg cttcgcgatt tatccgaaga cagatccgca ccgggtgttc ggcgtgggga
     2701 accggatgat ccacacgggc aagtggtgga cggggtatca gctcgcgaac acgctcgagg
     2761 cccgtgacct gcagcccgtc tgtgtaagcg atggggattc cttccagaac gcgattctgg
     2821 gggcgtaccg tgtggaacat acgaccgtcg actcggccgg ccccacgaac gtgccgcacc
     2881 tgaacaaccc ggtcgcgcgg atgctcgaca tcttctcgcc gaccgggcgg gtcgaactcg
     2941 tccgccgcgg cgaggagacg ggacgccgcc tcgccaagga cgccggtgtg ttggcgcttg
     3001 cactgggggg cgagaagccg agcttcaacg cagggcgggt gtgtcccacg gaatgggcgg
     3061 atgccgactt ccgagccttc tgccgcgcac gctacacgaa cgaccttgcc gccgcaaacc
     3121 gcgtgtgggg acgcaccttc acggactggc gcgaggtgcg tcagccgatc tacggcgtac
     3181 agtccgccgc aactgaaaag acgggcgccg ccgcgatcga ctggtacgcg aacatgggca
     3241 acatgagcga tgagttcgtg aaagagatca atcgacccga gaacgtcgag ttcgcgatgg
     3301 actggtaccg ctggatcacg aaggcctcga tcgagatgta cgccacgtac gcggatgcgg
     3361 cgcggcggca cgaccgcaag acgctctaca cgaacaatta ttgctggccg aacttcttcg
     3421 cccatctctg tctgcccacc tggcggcgtc tggatgcggc cgcgctcgac ttccagtatg
     3481 tctgcacctt cccgcgtacg ctcgggagta acagcgagat gatcgagatc gccgagatga
     3541 cggagtccgc gatggggaac aagcccatct acggtttcga ggtctacgtt cagccgcatt
     3601 acccggccga gatggcggcg ctccagaatt gggcgatggt tgcgcacggg atgcgtgtga
     3661 acctcgtctt cgcctggaaa ccgtattcgg actacggcgg caaggtgttc gagaacggcc
     3721 cgaaggcctg gacgcgtcct gcggacgcga aaccgatgtg gatgattatc gacacggacg
     3781 gaacgcgtct gccctgctat gcggccgtaa agcgttcaag cgaggagatt gccgcctggc
     3841 attctcaatt cgatgcgctc agtgtccgcc gtacgcgcgg ccgcacggct gtttaccttt
     3901 cggacgagac ggggatgtac acgatgttgc ggacgggaaa ccgcccctac ctcgagaacc
     3961 acctctgcca tgcgcgtaat cgaattgcga gcgggctccg tctcggcggt gtccgcattg
     4021 agtacttgga cgacgagacg ctcgaaaaac tggtccgccg ggacttcgac gccgtcgtac
     4081 tgccgcccac accgcggatt gcgccggaat ccgcacggcg cctggacgca ttcgagcgtg
     4141 ccggcggcga ggtgatccgc ctcgacgact ggtccgccga cttcttcgac cgctatccgc
     4201 agattccgcg cagcgcctac tggcgtgcgc cggatggacg ggtgaacgac gtggtggttg
     4261 tggtacgcac gcaggagggc acgggccgcc gttttgtctt cgtgctcaat cgcacgggcg
     4321 aggcggttga gggtgaactc gcgggccgag actttacgga agggcagacc ttcgccgata
     4381 cgctccgtgg tgggaaggtt ggtcgttcct tccgtcttga cggctggggt tatcgtgtgc
     4441 tcttgtgcga ctgatcgtgt tttgcaacgg acaatgaata tttcccaagg ccgatcttct
     4501 cctttcggga tcgatgaaga cctgaaaaac ggggtaagtc caatcttgtt tcccctgacg
     4561 ggatgagttg ttttgctttt gggattcccg accgcaagga gctgctgccc tggttcacgg
     4621 agcgcttcgc gccggtctac
//
LOCUS       DAOSRB010000212         4630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_16861_length_4630_cov_7.013989, whole genome shotgun sequence.
ACCESSION   DAOSRB010000212 DAOSRB010000000
VERSION     DAOSRB010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16861_length_4630_cov_7.013989"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLZ74_09635"
     CDS             complement(<1..512)
                     /locus_tag="PLZ74_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR98623.1"
                     /translation="MTFLFRLLREIPPRLLGSFVIKAGLGNLRAIRRFEQRKKQGAVF
                     PPFLFISVTQRCDLACKGCWATGVEKPVDMSEALLHQIVTEGREQGCRFFGILGGEPL
                     LLPWLPDFFRRHRNLYFQLFTNGQRLDDALARKLRKAGNVSPLISIEGNALSYAARRG
                     AGTAYETALTA"
     gene            complement(1051..2058)
                     /locus_tag="PLZ74_09640"
     CDS             complement(1051..2058)
                     /locus_tag="PLZ74_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98624.1"
                     /translation="MDIEFHYYITHLIAARAGVRGEALRIIPHACQLTDDNDQVYTIR
                     NGRQGIYRNRISQTFQPLNSHRERLAVYPLFHFIPGDPHAPGSARVDGQTHPFNTTPN
                     SPLANRILDATLATHDPYQIGIACHAYADTWAHQNFVGFKCDFNRFDDFVARLIPNIG
                     HADVQTRPDQPRLVWRDERLMVPVVNNKQRFLDAAAALFAKLRAWAAPQHPDSESEHR
                     ALRTDLDTAIGTDPGEETADAARLAGYRQLAEHPAYGATPIPEYAPDAWFRSAVRVIR
                     QRGGNPGPGRRMRKGYVFRTGYEFSDWYRFQNAVVSYAERTRALLCEIPALAEEYRRA
                     Y"
     gene            complement(2069..4099)
                     /locus_tag="PLZ74_09645"
     CDS             complement(2069..4099)
                     /locus_tag="PLZ74_09645"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOR98625.1"
                     /translation="MIRSALPHLLQTLLLAALTAAPSLSLCAEDVAVSPPLDWTLGAG
                     DLKGPLTFFGAETMRTTNVVWQGRDALTATWTGHPEYGNAFTVTVTWQRAPDGLWSGV
                     LAYSGYRGSRFIEEIHFPRLSTACDTHAAFLFGGNHSGRLYAVGSGPLKPGSRFRRDY
                     CGGMQFSALLRTNGTSLYLDHRDPAVSVKECEIAIRADGRRFTYTGIHIPGLPEKPAA
                     DYRIPYPSCHTLFQGGWFEAGQLYKKWGTAQSWHASRQGVNPMRKIGIWVWNRGLIKD
                     VLPPVERLQKELGAIPVALDWYWWHNNPYDTDYPDFWPPREGVEPFRAAVARLKRQGI
                     YSQVYVNGVCWDMDGKTWHQGGAQDVVVLRNGQPRNTAFNKYNLHRLAYMCGEAPHFQ
                     DKIASLMRHLCDSGLDGQYLDMIGNSTMRYRCYNPLHRHPKGGGTYGKDGFRALLERI
                     RRENPNYPLTTEGCNEAYMDLMDGSIVCNSTGLDRLDALPLFMSVYHGRYAFFGNYAY
                     PDGTRPWDPLWPPEDRWKEEKPWHRLYPDQFFAELARTVSWGCQPMVCNIKETLFTDP
                     DLVPNLRFILDSARFYHANLDVLFDGQMLSPAGFTCATAGVDYLIRSIFTKEHECKPQ
                     HAKLPAVLHSYWQAPDGKKALILANWTRASQPWSFNGLSGVIPARSYARIAP"
     gene            complement(4123..>4630)
                     /locus_tag="PLZ74_09650"
     CDS             complement(4123..>4630)
                     /locus_tag="PLZ74_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98626.1"
                     /translation="RPAEALALLQADEAAALAPAANAPWRKAFLDAAKPFDVRAALVI
                     GKQAVANGDDTLIHVDVDIPETPLNLFSHLAVKLTLNNTSTASMGKMGRLTSNWMAHV
                     EATNKKLLDRSTRLIVELAGVDYETACIALFESLEEMKTWDEARKKTVSPAAYTVKRL
                     TARAKPGK"
BASE COUNT          887 a         1323 c         1518 g          902 t
ORIGIN      
        1 gctgtcagcg cggtttcgta ggctgtgccc gccccccgcc gtgccgcata ggagagggca
       61 ttcccctcga tgctgatgag cggggagacg ttgcctgcct tgcgcaactt gcgggccagc
      121 gcgtcatcca gccgttgccc gttggtgaag agctggaaat agagattgcg gtgccgcctg
      181 aaaaaatcgg gtagccacgg cagcagcagc ggttcgcccc ccagaatgcc gaaaaatcgg
      241 cagccctgtt cgcggccctc ggtgacaatc tggtgcagca gcgcctcgga catgtcaacg
      301 ggcttttcca ccccggtggc ccaacaccct ttgcaggcca gatcgcagcg ttgggtcaca
      361 ctgatgaaga gaaaaggcgg aaagaccgcg ccctgttttt tgcgttgctc gaaacgccgg
      421 atcgcccgca gattgccaag cccggctttg atcacgaagg acccgagcag gcgcggcgga
      481 atctcgcgta gaagtcggaa cagaaacgtc attctcgctt atcccctttc ggtgcggatc
      541 ggacccggaa cagatcgcgg gtcaagtgag tcaagcatag gcgatttccg ctcccggcgg
      601 caatcccgtt tcaaggggat cacgcgctac tgaaaccctg aaaccttgaa acgatcgatc
      661 ccgatagcga tcccgatagc gatcccgatt ggaatggaac catgaaacac atgaaacttc
      721 atgaaaatca ggaattccct ctggcgttcc aaaaaaaccc gagcagtgcc atcccgtgtg
      781 ttcgtggtta aataatcgat cccgatagcg ataccgatag cgatagcgat ttggatgaaa
      841 ccctgaaacc ttgaaaccct gaaaccaaag agagttgaag gttcaactgc tctttttagg
      901 ttaagcaatc gatcccgata gcgataccga tagcgatagc gatttggatg aaacccgaaa
      961 ccctgaaacc ctgaaaccct gaaacacatg aaacttcatg aaaagcaggg gcattttggt
     1021 gttcgacgtg cgatgctgtt tgtggccgga ttagtacgcg cggcgatact cctccgccaa
     1081 ggcgggaatc tcgcaaagga gggcgcgggt acgttccgcg taggacacga cggcgttttg
     1141 gaaccggtac caatccgaaa actcgtatcc ggttctgaac acatatcctt ttcgcatccg
     1201 tctaccgggc cccgggttgc cgccccgttg ccggatcacc cgcactgcgg agcggaacca
     1261 ggcgtccggt gcgtattccg gaatcggtgt cgcgccgtat gcagggtgtt ccgccaactg
     1321 ccggtaaccc gcgaggcgtg cggcgtcggc cgtctcctcg ccggggtctg tgccgatcgc
     1381 cgtatcgagg tcggtgcgca gtgcgcggtg ctcgctttcc gaatcggggt gttgcggtgc
     1441 ggcccaggcg cggagtttgg cgaacagggc cgcggccgcg tcgagaaacc gctgcttgtt
     1501 gttcaccacg gggaccatga ggcgctcatc acgccacacg agccggggct ggtccggccg
     1561 ggtttggacg tcggcgtggc cgatgttggg gatcagccgg gcgacgaaat catcgaaccg
     1621 gttgaagtcg catttgaacc cgacgaagtt ctggtgcgcc cacgtgtccg cataagcgtg
     1681 gcatgcgatt ccgatctgat acggatcgtg ggttgcgagg gtggcatcca gaatccggtt
     1741 ggcgaggggg ctgtttggcg ttgtgttgaa cgggtgtgtc tgtccgtcca cgcgtgcgct
     1801 gcccggggca tgagggtcgc cggggatgaa atggaagaga gggtagaccg cgaggcgctc
     1861 ccgatgcgaa ttcaacggct ggaacgtctg cgagatccgg ttgcggtaaa ttccctgtcg
     1921 gccgttacgg atcgtataga cctggtcatt gtcatccgtg agttggcagg catgagggat
     1981 gatccgcaac gcctcacccc ggacacccgc ccgcgcggcg atcaggtggg tgatgtagta
     2041 atggaactcg atgtccatac gcggtgtttc atggggcgat ccgggcgtag gaacgcgcgg
     2101 ggatcactcc ggaaagcccg ttgaaagacc aaggttggct ggcgcgggtc cagttcgcga
     2161 ggatcagcgc cttcttgccg tcgggcgcct gccaatatga atgcaggact gccggtagtt
     2221 tggcatgttg gggtttgcac tcatgctcct tggtgaagat actgcggatc agatagtcta
     2281 cacccgccgt cgcgcaggtg aatccggcag gggagagcat ctgtccgtcg aacagaacat
     2341 ccagattggc gtggtagaag cgcgcgctgt cgagaatgaa gcgcaggttg gggaccagat
     2401 cgggatcggt gaagagggtc tctttgatgt tgcagaccat aggctggcag ccccacgaga
     2461 cggtccgcgc cagttccgcg aagaactggt cgggatagag gcggtgccag ggtttctcct
     2521 ctttccagcg gtcctcaggc ggccacagcg gatcccacgg ccgcgtgccg tcgggataag
     2581 cgtagttgcc gaaaaaggcg tagcggccgt gatagaccga catgaacaga gggagcgcgt
     2641 cgagacggtc cagaccggtg gagttacaga cgatcgaccc gtccatgaga tccatgtacg
     2701 cctcgttgca gccttcggtg gtcagcggat agttcgggtt ctcgcggcgg atgcgttcga
     2761 gcagtgcacg gaagccgtcc ttcccgtagg tgccgccccc tttcggatga cggtggagcg
     2821 ggttgtagca ccggtagcgc atcgtggagt tgccgatcat gtcgagatat tgcccgtcca
     2881 gaccgctgtc acacagatgg cgcatcagcg acgcgatctt atcctgaaag tgcggggcct
     2941 cgccgcacat gtaggctaga cggtgcaggt tgtatttgtt gaaggcggta ttgcgcggct
     3001 gaccgttccg cagcaccacc acgtcttggg caccgccctg atgccaggtt ttgccgtcca
     3061 tatcccaaca gacgccattg acgtagacct gcgagtagat gccttgccgc tttaggcggg
     3121 cgacagcagc gcggaacggc tccacgcctt cgcgcggcgg ccagaaatcg ggatagtcgg
     3181 tgtcgtaggg gttgttgtgc caccagtacc agtccagagc cacgggaatc gcgcccaact
     3241 ctttttgcag ccgttccacc ggcggcagca cgtctttaat aaggccgcgg ttccagaccc
     3301 agatgccgat cttgcgcatc ggattgacgc cctgacgcga ggcgtgccac gactgggcgg
     3361 tgccccactt cttgtaaagc tggcccgcct cgaaccagcc gccctgaaag agagtgtggc
     3421 agctcgggta ggggatacgg tagtctgcag ccggtttctc gggaagcccc gggatgtgga
     3481 tgccggtata ggtgaagcgg cgaccgtcgg cgcggatggc gatctcgcac tctttcacgc
     3541 tgaccgccgg gtcgcggtgg tccagataaa gcgatgtccc gttcgtgcgg agcagggcgg
     3601 agaattgcat gccgccgcaa tagtcgcggc ggaaacggct gcccggcttg agaggtccgc
     3661 ttcccacggc gtacaggcgg ccactgtggt tgccgccgaa cagaaaggcg gcgtgcgtgt
     3721 cgcatgcggt gctgagccgg ggaaagtgaa tctcctcgat gaaccgggat ccccggtatc
     3781 cggaataggc cagaacaccg ctccacaggc cgtccggagc ccgttgccaa gtcacggtca
     3841 cggtgaatgc atttccgtac tctgggtggc ccgtccatgt ggcggtcagc gcatcacggc
     3901 cttgccacac gacattcgtg gtccgcatgg tttccgcgcc gaagaaggtc agcgggcctt
     3961 tcaagtctcc tgcgccgagg gtccaatcga gcggcggcga caccgccaca tcctccgcgc
     4021 atagagacaa ggatggggcg gctgtgaggg ccgccaagag gagcgtttgg aggaggtgcg
     4081 gcagggcgga acgaatcatc gcggacacct ttcaggtacg gctcattttc cgggtttggc
     4141 acgggcggtc aggcgtttca ccgtatacgc cgcaggcgag acggtttttt tgcgggcctc
     4201 gtcccacgtc ttcatctcct ccagcgattc gaagagcgcg atgcaggcgg tctcgtaatc
     4261 cacgccggcc agttcgacaa tcaggcgggt gctgcggtcg agcagtttct tgttggtggc
     4321 ttcaacgtgc gccatccagt tactggtgag ccgccccatc ttgcccatcg aggcggtcga
     4381 ggtgttgttg agcgtcagct tgaccgccag gtgcgagaag aggttgagcg gggtttccgg
     4441 aatgtccacg tccacatgga tgagcgtgtc atcgccattc gcgacggcct gtttcccgat
     4501 gaccagcgcg gcccggacat caaagggttt ggcggcatcg agaaaggcct tgcgccacgg
     4561 cgcgttcgcg gcgggtgcca gcgcggcggc ttcgtcggct tgcagcagcg ccagggcctc
     4621 ggcagggcgg
//
LOCUS       DAOSRB010000213         4616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_16929_length_4616_cov_5.967989, whole genome shotgun sequence.
ACCESSION   DAOSRB010000213 DAOSRB010000000
VERSION     DAOSRB010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16929_length_4616_cov_5.967989"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1555)
                     /locus_tag="PLZ74_09655"
     CDS             complement(182..1555)
                     /locus_tag="PLZ74_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOR98627.1"
                     /translation="MNLNRRTFLQNSVCASASVVWGRYSTDTRPPVTPTPAQQTWHDL
                     ELGMFFHFDIPIYKQEWKWRSFTDFPHPDLYQPAKLDTDQWMEAAKAMGARYAIFVAK
                     HCSGFLQWQSDLYPYGVRQSSWEGGKGDVVRRFVDSCRRYGIRPGLYASVSANGYLGV
                     DNPGLVNRGKGGDDAAQARYVAICERMMTELWTRYGDLFEIWFDGGAIPVSKGGPDLV
                     PILKRHQPNAIVFQGPVGTPNLIRWVGNERGVAPYPCWSTAHAGTSEGGTIEKVFGGH
                     PDGNLWVPGECDVPVRNHDWFWKPGGEKRLYSDAHLIEMYYNSVGRNCNLLLNANPDP
                     DGLVPEADFQKYVTFGKEIRRRFDRPVAETRGAGTLVTLSLPQPQPVNHVMLMEEIRH
                     GERIRAYCVEGQIPGGVWQPLCTGISVGHKRIQTFDRTAVSSIRLHVTETVGEPLIRR
                     LAVFDVT"
     gene            complement(1552..2778)
                     /locus_tag="PLZ74_09660"
     CDS             complement(1552..2778)
                     /locus_tag="PLZ74_09660"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOR98628.1"
                     /translation="MTDTLRITDLTGRKTVQQTFQSGALHVISRSGLHAAERLLLDHL
                     GSLTPVPAHLLVAGNRTGVAAMAAALRFPDCAVTCHAFDLHHARAIARNLTANAFSCR
                     FCCDPGVTVPDAVRPPTGDGNGSAPVSLACTATLPDGPFDAALFMSTPGSTTGELLLD
                     QLEELHRALAPGGTCLIACETHVPALLKQVKRTFGPPAVLFHHGPLCCLSARKRGALA
                     NPRDFTASFAASLPGGASHTLYSLPGVFCHRRPDTGGLALAEVAARDLKPGARVLDMG
                     CGCGLVGLLLAAHQPDARVTFVDSHSRALAATRRNLDALGLTAESLVLSDSGLAVTGF
                     DLFAGNPPYYSDFRIADCFLRTARAALTPGGVCLTVAKTAHALAERQRELFGAAEILP
                     RRGYGVVRSVQQGEPS"
     gene            complement(2819..4561)
                     /locus_tag="PLZ74_09665"
     CDS             complement(2819..4561)
                     /locus_tag="PLZ74_09665"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOR98629.1"
                     /translation="MSLCETPLPDACGQELQRLIAEVPDADLSRLVATEPAFRVGGFR
                     PAGKPAVLRARLRQIVTGAGPLPESVRRILARRSRAAALLNLLSPELLAELRHALASL
                     LGDPALRIAMLLDDRVEVRDQAEAWLREADPLPPLKPDQALDRLREQFAPLTDLIGIA
                     PAANQPPTREAWLTQKARMEERLRCLQTEKRRLQGVEDRLARQATLLKKSEAELDLAR
                     QKAEAAEASLRRNTVELETARAELARETDRRDERLAAALDLALAREFHGWLAHTREVE
                     TEARETDRHADLVAQAEAALQHQREQDRHSGNRAVLGMRAEQLRSVLEEVRDTLAHAL
                     RVTPELQRMESALASEVRRIEQILNPQPPTTPLEKALLAHLHAAPENDLPGLRDLPER
                     FAPLGVLDDAALDRLREAFRKRLSVLQTAVPPPEPHMAERRGPVARLGRALAGEEPAI
                     LLIDGHNVLFGLPSRYAPPRGASLSEAEKRQRLIDDLTRLTSNTPSIRVWLVFDGPTR
                     SDNQAAANVRVTYSGGEGEHRADGVLLDTLRFFKSSSPDTVVFLVSNDHDLCVAAQRL
                     GAIDITVLELGPFF"
BASE COUNT          824 a         1380 c         1586 g          826 t
ORIGIN      
        1 gtgttctgtg gttaaacagt cgatcccgat cccgatagcg atagcgaccc cgatttggat
       61 ggaaccttga aaccctgaaa ccctgaaacc ctgaaaccca gaaaccaaag agagttgaag
      121 gttcaactgc tctttttagg gtaacccgtt tccttcctcc ccgcacggtt gaccgtgcgc
      181 ctcaagtcac atcgaatacg gccagacgac ggatcagcgg ctcgccgacc gtttcggtca
      241 catgcaggcg gatgctggac acggcggtcc ggtcaaaggt ctgaatacgc ttgtgcccca
      301 cggaaatgcc ggtgcagagc ggttgccaga cgccgccggg aatctggcct tccacacaat
      361 aggcgcggat ccgttcgccg tgacggattt cctccatgag catcacatgg ttcacgggtt
      421 gcggctgggg caggctcagc gtgacgagtg tgcccgcgcc gcgggtctcg gcgaccggcc
      481 ggtcaaaacg gcgtcggatc tctttgccga aggtcacata tttctggaaa tccgcctccg
      541 gcacaagccc gtccggatcc gggttggcgt ttagtaacag attgcagtta cgccccacgg
      601 agttgtagta catctcgatc aggtgcgcat cggagtagag ccgcttctcg cctcccggtt
      661 tccagaacca gtcatggttg cgcaccggca catcgcattc gccgggaacc cagaggttcc
      721 cgtccgggtg gccaccgaag actttctcga tcgtgccgcc ttcgcttgtg cccgcatggg
      781 ccgtgctcca gcagggatag ggcgcgacac cccgttcgtt gccgacccac cggattaggt
      841 tcggtgtgcc gaccggaccc tgaaaaacga tggcgttggg ttgatggcgt ttgaggatcg
      901 gcaccaaatc cggaccaccc ttgctgaccg ggatggcacc gccgtcgaac cagatctcga
      961 acaggtcgcc gtagcgtgtc cagagttcgg tcatcatccg ttcgcagatc gccacatacc
     1021 gtgcttgggc cgcgtcatcc ccgcctttgc cgcgattcac aagcccgggg ttgtctacgc
     1081 ctagataccc gttggcggag acggacgcat aaaggccggg acggatgccg taccgtcggc
     1141 aggagtccac aaaccgtcgc accacatcgc ccttgccgcc ttcccacgag gattgcctga
     1201 ccccgtaggg gtagagatcg gactgccatt gcaggaaccc gctacagtgt ttggccacaa
     1261 agatggcgta ccgcgcaccc atggccttgg ccgcctccat ccactggtcc gtgtccagtt
     1321 tggcgggctg atacagatcg ggatgtggaa aatccgtgaa actacgccat ttccactcct
     1381 gcttgtagat cgggatgtcg aaatggaaga acatgcccag ttccagatcg tgccaagtct
     1441 gttgggcagg agtgggcgtc accggcggac gcgtatcggt tgaatatcgc ccccagacga
     1501 cggacgcgga cgcgcagacg gagttttgca gaaaggttct tcggttcagg ttcatgatgg
     1561 ttctccttgt tggacgcttc ggaccacgcc gtacccgcgg cgtggcagaa tttcggctgc
     1621 cccgaacaac tcgcgctgtc gttccgcaag cgcatgggcc gttttggcga ctgtgaggca
     1681 gacgcctccg ggcgtgagag cggcgcgggc ggttcggaga aagcaatcgg cgatgcgaaa
     1741 atcggagtag tagggcggat tcccggcgaa caggtcgaat ccggttacgg ccaggcccga
     1801 atcggagagc accaggcttt ccgcggtgag cccgagggca tcgaggttgc ggcgcgttgc
     1861 ggccagggcg cggctgtggg aatccacgaa cgtgacccgc gcgtccggct ggtgtgcggc
     1921 cagcagcaga ccgaccagac cgcagccaca ccccatgtcc agcacgcgcg cgccgggttt
     1981 gagatcacgc gccgcgactt cggcaagcgc gaggcctccc gtgtcagggc ggcggtggca
     2041 aaagaccccc ggcagcgagt agagtgtgtg ggaggcccca ccgggaaggg aggccgcgaa
     2101 cgatgcggtg aagtcgcgcg ggtttgcgag cgcaccacgc ttgcgcgcgc tgagacagca
     2161 gagcgggccg tgatggaaaa ggactgcggg ggggccgaac gtgcgtttga cctgtttgag
     2221 cagggccgga acatgggttt cgcaggcgat caggcaggtc ccgccgggcg cgagggcgcg
     2281 atggagttct tcaagctggt cgagcagcaa ctcgccggtt gtgcttcccg gcgtggacat
     2341 gaagagcgcg gcgtcaaacg ggccgtccgg cagggttgcg gtacaagcga gagaaaccgg
     2401 cgcactgccg ttgccgtctc cggtcggggg gcgcaccgca tctggcactg tgacgccggg
     2461 gtcgcaacaa aaacggcagg aaaacgcatt ggccgtaaga ttgcgcgcga tggcccgtgc
     2521 gtgatgcagg tcgaaggcgt gacaggttac ggcgcagtcc ggaaaacgca gggcagccgc
     2581 catggccgcc acgccggtgc ggttgcccgc gaccaacagg tgcgcgggaa caggcgtgag
     2641 gctcccgaga tgatcaagca acagcctttc ggcggcgtgc agtccggaac gggagatgac
     2701 gtgcagcgcg ccgctctgga acgtctgttg gacggtcttg cggccggtga ggtcggtaat
     2761 gcggagggta tcggtcatgc agagggtttc gatttccgtt ggattggggg tgcccgtgtc
     2821 agaagaaggg cccgagctcc agcacggtga tgtcgattgc accgaggcgt tgtgcggcga
     2881 cgcacaggtc atggtcattg ctgaccaaaa acaccacggt gtccggggag gagcttttga
     2941 aaaaacgcag ggtgtcgagc agcacgccgt cggcacggtg ttccccttcg ccacccgaat
     3001 aggtgacacg cacatttgcg gcggcttggt tgtcgctgcg ggtgggtccg tcgaacacga
     3061 gccagacacg gattgaaggc gtattagagg tgagccgcgt cagatcatcg atgagccgct
     3121 gtcgtttctc ggcttccgaa agcgaggccc cgcgcggcgg cgcgtagcgg gagggaaggc
     3181 caaacaacac attgtgtccg tcgatcagca ggatggcggg ttcctctccg gctaaagcgc
     3241 gacccagccg ggctacgggg ccgcggcgtt cagccatgtg gggttccggg ggcggcacgg
     3301 cggtttggag aaccgacagc cgtttccgga aggcctcgcg caggcgatca agggccgcgt
     3361 catccagcac gccgaggggg gcaaagcgtt ccggcaggtc acgcagtccc ggcaggtcgt
     3421 tttccggtgc ggcgtgcagg tgcgcgagaa gggccttttc cagcggggtg gtcgggggtt
     3481 gaggattgag gatctgttca atccgccgca cctccgaggc gagggcggat tccatgcgtt
     3541 ggagttcggg ggtcacccgt aacgcgtggg cgagggtgtc gcgcacctcc tccagaacgg
     3601 accggagctg ttcggcacgc attccgagta cggcgcggtt tccgctgtgc cgatcctgtt
     3661 cccgctgatg ctgcagggcc gcttcggcct gggctacgag gtctgcatgc cggtcggttt
     3721 cacgggcctc cgtctccacc tcgcgcgtgt gcgcgagcca gccgtggaat tcccgggcca
     3781 gtgcgagatc cagagccgcg gcgaggcgtt cgtcacgccg gtcggtttcg cgggcgagtt
     3841 cggctctcgc tgtctccaac tcaacggtgt tccgtcgcag actggcttca gcggcttcgg
     3901 ccttttggcg cgcgagatcc aattccgcct cgctcttttt caggagtgtg gcttggcgcg
     3961 cgaggcggtc ctccacacct tggagacgac gtttttcggt ttggaggcag cgaagccgct
     4021 cttccatgcg cgctttctgg gtcagccagg cttcacgcgt gggaggctgg tttgcggcgg
     4081 gtgcgatacc gatgagatcc gtgagcgggg cgaattgctc gcgcaggcgg tcgagcgcct
     4141 gatccggctt caggggcggc aagggatcgg cctcgcggag ccacgcttcc gcctgatcac
     4201 gcacctcgac gcggtcatcg aggagcatcg cgatccgcag tgcgggatca ccgagcaggg
     4261 acgccaaggc gtggcgcagt tcggcgagga gttcggggga gagcaggttc agcaacgcgg
     4321 cggcgcgcga ccggcgcgcg agaatccggc gcacggattc gggaagcgga cccgcacccg
     4381 tcacgatctg tctcagccgt gcgcggagca cggcgggttt gccggccgga cggaagcctc
     4441 cgacgcggaa tgccggttcg gtggccacga gccgcgacag gtccgcgtcc ggcacctctg
     4501 cgatcaagcg ctgaagctcc tgtccgcatg cgtccggcaa gggtgtttca caaagagaca
     4561 taacggaacc gttatgatag gccgttacgt ccctgcaggc aatccgaatc ccccgg
//
LOCUS       DAOSRB010000214         4594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17036_length_4594_cov_7.640009, whole genome shotgun sequence.
ACCESSION   DAOSRB010000214 DAOSRB010000000
VERSION     DAOSRB010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17036_length_4594_cov_7.640009"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1136)
                     /locus_tag="PLZ74_09670"
     CDS             complement(99..1136)
                     /locus_tag="PLZ74_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOR98630.1"
                     /translation="MKNKCLWGLVAGVLSGIVPHAAAQVVSSVAMPVIQLTGTNEVEL
                     GKITCEELKLITFVFRNPGSVPVEIKQITSTCPCIRGYPEQAVIPPNGELPITAELNP
                     LKVKSVFKRGLWIHTTDPRQVRIPLIVSGEVVPFFEGFPQEPVQFRRGAPGEAWTNVF
                     PLTATLTNLSLGTPVITKSKDMRVTASVAANQAQAGSFDLTLVAVPLVAGRSSATVEI
                     PIAGRPDLPNLDLLLYARVGTELYVTPSKFLMLPSDQPLTRGYLIRTGETEADPKQVA
                     FSPEREGVTYDVRPSKRKKAHLSLRVTVTPDALARLMREKEPKLRISYPNYKEAEITF
                     IPHMPPGPEQK"
     gene            complement(1497..3566)
                     /locus_tag="PLZ74_09675"
     CDS             complement(1497..3566)
                     /locus_tag="PLZ74_09675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR98631.1"
                     /translation="MSDSPQTMKMLPGDEIRQIMWRYADRYDIQMAVMGARSVARGLV
                     ARLVADGERATHEWTEAKNSLYQAFDESGITAAGLDMEYGGIIEGPRNLALSLIAFEL
                     SWVDGGASTTSLINNLGLGPIVEKGTPEQREKYMRVCAPAQPGEDRKPWRAAFALTEP
                     LPYVGVDTGVVSGRVTVAKWEEGQEPILHVEKRGRFLTNMAVANVLTAAVDTGDPRIK
                     SSCMVILEDTDPGVFDRGAPTLKMVHQLSNTHDPIFSLDVPASRIVGGYTVKDGVIVP
                     NLTHSEIIEAVFSRTRVGVGLMTAAKLLSAVEPVIRYHRGRYRGAAGIEAGAPRYDQG
                     IQMKEDAVQRLADVWATGEAAASLGFETARAFDALTPVENQALADFAAQGVSGRALMR
                     ALRKPQADAIELLDQLGKPEAERDEARVAALQADPLVQYVWLSALCNVLCPATKLWDT
                     GHGANMLREAVSLMGGYGITEDCPGFLFYKWTDAQLEATYEGPEVVQRRQISVTMNNE
                     VFLAQVAQWIAELRRQNAAAPGNGLDTLAEGFALWRWTLAFIQSAKDAEGRALSQSQR
                     HGVLFPMADAISWLLAARSLVADIQELAAKGPGHPVVGPEIAGYVNTFTDLAHMQIAR
                     AVGETARICAELVYGYGAATAEQSSEFQTLRAKADAAMAGARLAKDRAGRALAQVMIP
                     EALDYPQ"
     gene            3838..4590
                     /locus_tag="PLZ74_09680"
     CDS             3838..4590
                     /locus_tag="PLZ74_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98632.1"
                     /translation="MKFLKNIPPPETFRDFSEPRAFSIWMRWACGFAAGVCLIGLAVC
                     VALDGCGKRKDGHDAETAEAEKPSVPPPDLHNPVREAPATFPPDSNLPRGKEIYASGP
                     IDLAQFDPARDLVRFEDERVWFESDHDGPEDDEDDHQIHRAVEVPLRRLVNLLERRGG
                     KLKVQEAYRAASSKNRIHLPSSLHREGRAVDLTCEGLPLSELAKLAWQAGFDFVLYEV
                     PKRSGPHLHCSVRRAPDAPLPPRPGGQSPQAP"
BASE COUNT          783 a         1372 c         1618 g          821 t
ORIGIN      
        1 gcggcgtttg ggttgttggc aaggcggcgg atccggcgga tcagtccgat cggactgatc
       61 cgccattgcg ggcgggaagc atcgctatcg ggcgcggctt atttctgttc cggacccggc
      121 ggcatgtggg ggatgaacgt gatttccgcc tctttataat tgggatagct gatgcgcagc
      181 ttgggctcct tctcgcgcat gagccgggcg agcgcatccg gagtgacggt gacgcgcagc
      241 gagaggtgcg cctttttccg tttgctgggg cggacatcat aagtcacgcc ctcgcgttcc
      301 ggcgagaagg ccacctgctt cggatccgct tcggtctcgc ccgtacggat cagatacccc
      361 cgcgtaaggg gctggtctga agggagcatg agaaactttg agggcgtgac gtacagttcg
      421 gttccgacgc gcgcatacag cagcaaatcc agattcggga gatcgggcct tcccgcgatc
      481 ggaatctcca ctgtggcgct actgcgcccg gcaaccagcg gcacggccac gagcgtcagg
      541 tcgaacgatc ccgcctgggc ctggttcgcg gcgaccgagg cggtgacacg catgtccttg
      601 ctttttgtga tgaccggtgt tccgagggag aggtttgtga gcgtggccgt gagggggaac
      661 acgtttgtcc acgcctcgcc gggcgcgccc cgcctgaact gcacgggctc ttgcggaaag
      721 ccttcgaaaa acggcacgac ctccccgctg acgatcagcg ggatgcgaac ctgccgcgga
      781 tcggtggtgt ggatccacag gccccgcttg aagacgcttt tgaccttgag ggggttcagt
      841 tcagcggtga tggggagttc accgtttggg gggatgaccg cctgctccgg ataaccccgg
      901 atacaggggc aggtcgaggt gatctgtttg atctccaccg gcacgcttcc gggattccgg
      961 aaaacgaacg tgatcagctt caactcttca caggtgatct tgcccagctc gacctcgttg
     1021 gtcccggtaa gctggatcac cggcatggcg acgctggaaa ccacctgagc cgccgcatgc
     1081 ggaacgatcc ccgacaacac gccagccacc aaaccccaaa ggcacttgtt tttcataagg
     1141 gaaaaacgat atcacatccc ggcaggcgca tcaatgcaat cggaaaaaag aaaggaggaa
     1201 ttaaaaagga ggcgggcaga aagtcgggag cgcgggaaag tcgggagcgc gggcgtcccc
     1261 gcccgctttg gtttcagggt ttcatggttt catggtttct ggtttcaggg tttcatccaa
     1321 atcggggtcg gggtcggtat cgctatcggt atcgctatcg gtatcgtcga cccgcgaccc
     1381 gcgacattcg acccgcggcg tttgggttgt tggcaaggcg gcggatccgg cggatcagtc
     1441 cgatcggact gatccgccat tgcgggcggg gacgcccgcg ctcccggctg aaaggatcac
     1501 tgcgggtagt cgagggcctc ggggatcatg acctgcgcga gcgcgcggcc agcgcggtct
     1561 ttggcgaggc gcgcgccggc catcgcggca tccgccttgg cgcgcagcgt ctggaactcg
     1621 gacgactgct ccgccgtggc cgcgccgtag ccgtaaacca gctcggcgca gatgcgggcc
     1681 gtctcgccga cggcgcgcgc gatctgcatg tgcgccagat cggtgaaggt gttgacgtac
     1741 cccgcgatct caggcccgac caccgggtgc ccggggcctt tggcggcgag ctcctgaatg
     1801 tcggcgacga gtgaacgcgc ggccagcagc cacgagatcg cgtcggccat cgggaagagc
     1861 acgccgtggc gctgactctg cgagagcgcg cggccttcgg catccttggc ggactggatg
     1921 aaggcgaggg tccagcgcca gagcgcgaag ccttcggcca gcgtgtcgag gccgttgccg
     1981 ggcgcggcgg cgttctgtcg gcgcagctcc gcgatccatt gggcgacctg ggccaggaag
     2041 acctcgttgt tcatggtgac gctgatctgg cggcgctgca ccacttccgg gccttcgtag
     2101 gtggcctcga gctgggcgtc ggtccatttg tagaaaagga agccggggca gtcttcggtg
     2161 atgccgtagc cgcccatcag gctgaccgcc tcgcgcagca tgttggcccc gtgtccggtg
     2221 tcccacagct tggtggccgg gcagagcacg ttgcagagcg cgctgagcca gacgtactgc
     2281 acgagcggat cggcctgcag cgccgcgacg cgggcttcgt cgcgctcggc ctcgggcttg
     2341 ccaagctggt ccagcagctc gatggcgtcg gcctgcggtt tgcgcaacgc ccgcatcagg
     2401 gcgcggccgg agacgccttg ggcggcgaaa tcggcgagcg cctggttttc aacaggcgtg
     2461 agggcgtcaa aggcgcgcgc ggtctcgaac ccgagcgagg ccgcggcttc gccggtggcc
     2521 cagacatcgg cgaggcgctg aacggcgtcc tccttcatct ggatgccctg atcgtagcgg
     2581 ggcgcgccgg cctcgatgcc ggccgcgccg cggtaacgcc cgcgatggta gcggatgacc
     2641 ggctcgacag cggagagcag cttggcggcg gtcatcaggc cgaccccaac gcgcgtccgc
     2701 gagaagaccg cctcgatgat ctcgctgtgg gtcaggttgg gcacgatcac gccgtccttg
     2761 accgtgtagc cgccgacgat acggctggcg gggacatcaa ggctgaagat cgggtcgtga
     2821 gtgttggaaa gctggtggac cattttgagg gtcggcgcgc cgcggtcgaa gacgcccgga
     2881 tcggtgtctt cgagaatcac catgcaggag gatttgatac gcgggtcgcc ggtatcgact
     2941 gcggcggtaa gcacgttggc gacagccatg ttggtcagga agcggccgcg cttctcgaca
     3001 tgcaggatcg gctcctgccc ctcttcccat ttggccacgg tcacgcggcc gctgaccacg
     3061 ccggtgtcca cgcccacata gggcagcggc tcggtgaggg caaaggcggc gcgccacggt
     3121 ttgcggtcct cgccgggctg ggccggggcg cagacgcgca tgtacttctc gcgttgctcg
     3181 ggcgtgccct tctcaacgat cggcccgagg ccgaggttgt tgatcaggct ggtggtggag
     3241 gccccgccgt cgacccagga gagttcaaag gcgatcaggc tcagcgccag attgcgcggc
     3301 ccttcgatga tgccgccgta ttccatgtcg agtcccgccg cagtgatgcc cgactcgtcg
     3361 aacgcctgat agagactgtt tttcgcctcg gtccactcgt gcgtcgcgcg ttcgccgtca
     3421 gccaccaagc gggccacgag gccgcgggcc acggaacggg cacccatcac ggccatttga
     3481 atgtcgtacc ggtcggcata gcgccacatg atctgccgga tctcgtcgcc gggcaacatt
     3541 ttcatggtct gcggagaatc gctcatcgtc agcacctttc ttgatcgaat ttgaggatga
     3601 cattttatgg ttttttgtcg aagacgcaag gggaaaggtt ggcggcatgg gagacgggag
     3661 acgggtggcg ggagacggga gacgggagac acgagacggg agacaagaga cgggagacaa
     3721 gagacgggag acaggagacg gaaaagggag gcggaaaagg gaggcggcac ttatttttgg
     3781 tttcattatg gagtcgcctt cagtatagtt cagggtttgt ttccgaagag gatgcgcatg
     3841 aagtttttga aaaacatccc cccgcccgag acattccggg atttcagtga accgcgggcg
     3901 ttcagtatct ggatgcgctg ggcatgcggg tttgcggcag gggtgtgcct gatcgggctg
     3961 gcggtttgtg tcgccctcga tgggtgcgga aagcggaagg acgggcacga cgccgagacg
     4021 gcggaggcag agaagccgtc ggtgccgccg ccggacctgc acaacccggt gcgggaggcg
     4081 cccgccactt ttccgccgga ctccaatctg ccgcgcggca aggagattta cgcatcgggg
     4141 ccgatcgatc tggcgcagtt cgatcctgcc cgcgatctgg tccggtttga ggatgagcgt
     4201 gtatggttcg agagcgatca cgacgggccg gaggacgatg aggacgacca ccagatccac
     4261 cgcgcggtgg aggtgccgct ccggcggctg gtcaatctgc tggagcgccg cggcgggaag
     4321 ctcaaggtgc aggaggcgta ccgtgcggca agcagcaaga accgcatcca cctcccctct
     4381 tccctgcacc gcgagggccg cgccgtggac ctgacctgcg aggggctccc gctcagcgag
     4441 ttggcgaagc tggcctggca ggcggggttc gactttgttc tgtacgaggt gccgaaacgc
     4501 tccgggccgc atctgcactg ctcggtccgg cgcgccccgg acgcgccgct gccgccgcgg
     4561 ccgggcggac agagtccgca agcgccttag cggc
//
LOCUS       DAOSRB010000215         4572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17163_length_4572_cov_12.102280, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000215 DAOSRB010000000
VERSION     DAOSRB010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17163_length_4572_cov_12.102280"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1661
                     /locus_tag="PLZ74_09685"
     CDS             306..1661
                     /locus_tag="PLZ74_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98633.1"
                     /translation="MAKLQTGITRRAWLGKSGFTAGGFLILPRSVIGASGGVPPSGKL
                     NIGCVGIGGQGGGVAKELATFPEVNLAAFCDVEAKHAERMRKTYPGRPFYSDYRVMLE
                     KEKGLDAVMIGTPDHWHAPIALAAMRRGKHVYVEKPMAHTIEEARRMGEVAAETGVVT
                     QMGNQGHAGEGLRLTKEWLDAGAIGAVREVHVWSDRPGTFWNTQGRRRPAETPPVPPT
                     LDWDLWQGPAPEHPYHPDYVPRRWRGWFDYGCGALGDMMVHNADPAWYALNLGAPDAV
                     EAVTSETNPDSFPQWSIVTWHFAAKGARGPVRVVWYDGGKRPAPPPGLEAERELGDNG
                     LIFLGEKGAMMCPGWSGTPRLVPESAMKTFERPPKTIPRSPGHRKEWVQACIAGRPEE
                     ARAGFHYSAPFTEALLVGVLPIRLGKRIEWDAAAMRAKNAPEADVLIRKPYRKGFGLN
                     G"
     gene            1879..2793
                     /gene="lipA"
                     /locus_tag="PLZ74_09690"
     CDS             1879..2793
                     /gene="lipA"
                     /locus_tag="PLZ74_09690"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOR98634.1"
                     /translation="MQEEPVRSTPARPSWIRVRVGQNERFQETRARLRTHGLHTVCEE
                     AFCPNLGRCWAHGRATILILGDRCTRGCRFCNVEKRSVPPPDPAEPQRVAAAVRETGL
                     KEVVLTSVTRDDLPDGGAAFWAETVEAVHQAVPGILVEVLVPDFAGDAEALDRVIRTR
                     PEVFGHNLETVPRLYPEARPQADYTRSLGVLRQAADAGLIVKTSLMLGLGETAAERLQ
                     VLRDARAAGCRIIYAGQYLQPSPRHLPVVQYVEPAGFDALHEAAIGLGFDYAACAPLV
                     RSSYHEEGQTRFVRDALTASRTRDPSLS"
     gene            complement(2756..3889)
                     /gene="thiH"
                     /locus_tag="PLZ74_09695"
     CDS             complement(2756..3889)
                     /gene="thiH"
                     /locus_tag="PLZ74_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HOR98635.1"
                     /translation="MPQHTPFLDLLAEWTPARVQELIASRTPEDVASALAAAGRYQPR
                     HLAALVSPAAEASLEPLARLSAHWTRQRFGHAVQFFAPLYVSNFCCNGCRYCGFNCNT
                     HNTVRRALTLEEAEAEAECLADQGFSHILLVSGEDPVNVPPSYFAELTRRIRHRFASV
                     NIEIYALSLEEYRMLAAAGVDGMTMFQETYHPEVYAACHPSGPKRDFANRLDAFERAA
                     QAGLTFIGLGSLLGINDWREEAFYLGLHAHFLQTRHWRASLAISFPRMRPAHGGTPPA
                     HPVSDTHFVQLLCALRTQLPDVTMTLSTRELPGFRERLVRLAVTKISAGAKTTPGGYT
                     EPTQAEAQFDVADRRPLHEVAAAIAALGFDPVMKDWDRAYGTP"
     gene            complement(3892..4302)
                     /locus_tag="PLZ74_09700"
     CDS             complement(3892..4302)
                     /locus_tag="PLZ74_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98636.1"
                     /translation="MKPSSREILCTACGQTTWARAEPVYDGFKKIGEAFLCTGCGARY
                     ASAAETPFVTASARPRVFTEQDQPVRPVVFREDERRHSCGWCRHFVVNPFAQRCGLTN
                     RHTEATDLCVRFESKPSTETDDTAPDESKDPPRS"
     gene            complement(4283..>4572)
                     /locus_tag="PLZ74_09705"
     CDS             complement(4283..>4572)
                     /locus_tag="PLZ74_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98637.1"
                     /translation="WPLRCPFVCVTQFDNMLSHREDNTTRVVLRVMYPLVRKMQEHFL
                     KKVDFFSDRFPRTATDPLFFLHSRAAALQCGLIHPTQMFPFSSHVYETLFS"
BASE COUNT          844 a         1308 c         1601 g          819 t
ORIGIN      
        1 tgcggtgtcg gaaacgcggg cgtccctgtc gatcgtcgcg ggtcgacgat cccgaccccg
       61 atcccgaccc cgatcccgat cccgaccccg atttggatga aaccttgaaa ccctgaaacc
      121 agaaaccctg aaaccaaaga gagttgaagg ttcaactgct ctttttaggt attgtcttcg
      181 cacactcctg tcacgaaccg attacggcgt cacccttccg cgaagtttac ggtggccggt
      241 gttttgtgat atataaatac ggatgtccgg gtggcggacg gatgaacgcg aaacggaggt
      301 gtaagatggc gaagcttcag acagggatca caaggcgcgc gtggttgggc aaaagcggat
      361 ttacggcggg cgggtttctg atcctgccgc gatctgtgat cggcgcgtcg ggcggggtgc
      421 cgccttcggg caaactgaac atcggatgtg tcggcatcgg cgggcaggga gggggcgtcg
      481 ccaaggagct ggcaacgttt ccggaggtca atctcgcggc cttttgcgat gtggaagcca
      541 agcatgcgga acgcatgcgg aagacctatc cgggacgtcc cttctactcg gactaccggg
      601 tgatgctgga gaaggagaag gggttggatg cggtgatgat cggcacgccg gatcactggc
      661 atgcgccgat cgccctggcc gcgatgcggc ggggcaagca tgtgtatgtg gaaaagccca
      721 tggcgcacac gatcgaggag gcgcgccgga tgggcgaggt tgccgcagag acgggcgtgg
      781 tgacgcagat gggcaatcag gggcatgctg gcgagggttt gcggttgacg aaagagtggc
      841 tggatgccgg ggcgatcggt gcggtacggg aggtccacgt ctggtctgac cggcccggca
      901 ctttctggaa cacgcaggga cggcggcgtc ccgccgagac cccgccggtt ccgccgactc
      961 tggactggga tttgtggcag gggcctgcgc cggaacaccc gtatcatccg gattacgtgc
     1021 cgcggcgctg gcgcggctgg ttcgattacg gttgcggtgc gctgggtgac atgatggtgc
     1081 acaatgccga ccccgcttgg tatgctttga atctcggcgc gccggatgcg gtggaggcgg
     1141 tcacctccga gaccaacccc gacagtttcc cgcagtggag catcgtgacg tggcatttcg
     1201 cggccaaagg cgcgcgcggg ccggtccggg tggtctggta cgacggcggc aagcgtccag
     1261 cgccgccgcc cggattggaa gccgagcggg agttgggtga caacggcctg atcttccttg
     1321 gcgagaaagg cgcgatgatg tgtcccggat ggtctggcac gccacggttg gtgcccgaga
     1381 gcgccatgaa aacgtttgag cgtccgccga agacgattcc gcgcagtccc ggtcaccgca
     1441 aggagtgggt ccaagcctgt atcgcgggcc gtccggagga agctcgtgcc gggttccact
     1501 attccgcgcc ctttaccgaa gcgctgttgg tcggtgtgct gccgatccgg ctcgggaagc
     1561 ggatcgagtg ggacgccgcc gcgatgcggg ccaagaacgc gccggaagcg gatgtgctga
     1621 tccgcaagcc gtaccgcaag ggattcggcc tcaacggctg agcgcggcgt caaggcatgc
     1681 agcgcgcggc gttgacccgc gacccgcgac attcggcctg cggcgtttgg gttgtttggc
     1741 agggcgacgg atccgctgtc gcgggcggga acgcccgcgt tcccgactga aaccggaaac
     1801 ccgtttcccg ctttggcgat gcgtcctgac gcgggtgcgg ggttggaaac tgcgacgcga
     1861 aaggatatga tacggcgcat gcaagaggaa cccgtacgct caaccccggc gcgtccatcc
     1921 tggatccgcg tgcgggttgg tcagaatgag cgttttcaag agactcgcgc gcggttgcgg
     1981 acacacgggt tgcacaccgt gtgcgaagag gcgttctgtc cgaacctcgg gcgttgctgg
     2041 gcgcacggcc gcgccacgat tctgattctg ggcgatcgct gcacgcgcgg ttgccgtttc
     2101 tgtaatgtgg aaaaacggtc ggtgccgcca ccggatccgg cggaaccgca gcgcgtggcc
     2161 gcggcggtgc gcgagaccgg tctgaaagag gtggtgctca cttcggtcac gcgggatgat
     2221 ctgccggacg gcggcgcggc gttttgggca gagaccgtcg aagcggtgca tcaggcggtg
     2281 cccggtattc tggtcgaggt gctggtgccg gacttcgcag gtgacgcgga ggcgctggac
     2341 agggtgatcc ggacgcgccc cgaggtgttc gggcataatc tggagacggt gccgcgcctc
     2401 tatcccgagg cccggccgca ggcggactac acgcgatccc ttggggtgct gcggcaggcg
     2461 gcggatgcgg ggctgatcgt caaaaccagt ctgatgctgg ggttgggcga aaccgccgca
     2521 gagcggttgc aggtgttgcg cgacgcgcgc gcggccggat gccgcatcat ttacgcaggg
     2581 cagtatctgc aaccaagccc gcggcatctg ccggtcgtgc agtatgtgga gccggccggg
     2641 ttcgacgcac tgcacgaagc cgcgatcgga ctcggctttg actatgcggc ctgtgcgccg
     2701 ctggtgcgca gctcttatca cgaggaaggg caaacccgct ttgtgcgcga cgcgctcacg
     2761 gcgtcccgta cgcgcgatcc cagtctttca tgaccgggtc gaagccgagc gcggcgatcg
     2821 cggcagccac ctcatgcaac ggacgtcgat ccgccacgtc aaactgcgct tcggcttgcg
     2881 tcggttccgt gtatccgccg ggcgtggttt tggcgcctgc ggatattttg gtcaccgcca
     2941 gccgcaccaa gcgttccctg aagcccggca actcgcgcgt tgagagggtc atcgtcacat
     3001 ccggcagttg tgtgcgcaac gcgcacagga gctgaacgaa atgcgtgtct gaaacagggt
     3061 gtgccggcgg tgtgccgcca tgcgccggac gcatgcgcgg gaaggagatc gccagagagg
     3121 cgcgccaatg acgcgtctgc aagaaatgcg cgtgcaagcc gaggtagaac gcctcctcgc
     3181 gccagtcgtt gatgccgagc agcgatccga gcccgatgaa cgtgagcccg gcttgcgcgg
     3241 cgcgctcgaa tgcgtcgagg cggttggcga agtcgcgttt gggtccgctc ggatggcatg
     3301 ccgcgtacac ctcaggatga taggtctctt ggaacatggt catcccgtcc acccccgccg
     3361 cggccagcat ccggtactcc tcgagggaga gcgcgtagat ttcgatgttg accgaggcga
     3421 aacggtgccg gatccgacgc gtcagctccg cgaaatagga gggcggcacg tttacgggat
     3481 cctcgcccga gacgagcagg atatggctga acccctgatc cgccagacac tcggcttcgg
     3541 cttcggcctc ctccagcgtc agagcgcggc gcacggtgtt gtgggtgttg cagttgaacc
     3601 cgcagtagcg gcagccgttg cagcagaagt tcgaaacata gagcggcgcg aagaattgca
     3661 cggcgtgtcc gaagcgctgg cgcgtccagt gcgcggagag acgggccagc ggttccagcg
     3721 aagcttccgc cgcaggcgag accaacgcgg ccagatggcg cggctgatac cgtcccgccg
     3781 cggccagcgc cgacgcgaca tcttcaggcg tacgcgaggc gatcagttcc tgaacgcggg
     3841 caggcgtcca ttcggcaagc aggtcgagaa aaggagtgtg ttgcggcatg gtcagctccg
     3901 gggcgggtct ttgctttcat ccggcgcggt gtcgtcggtt tcggttgacg gtttggattc
     3961 gaaacgcacg cagagatccg tggcttcggt gtgacggttg gtcaggccgc accgttgcgc
     4021 gaaggggttc accacaaagt gacggcacca gccgcagctg tggcggcgct catcctcacg
     4081 gaagacaaca ggccgcaccg gttgatcctg ttccgtgaag acgcgcggac gcgcacttgc
     4141 cgtcacaaag ggggtctccg ctgcggaagc gtagcgcgcg ccgcaacccg tgcagagaaa
     4201 cgcctcgccg atttttttga agccatcgta aaccggttca gcgcgggccc atgttgtctg
     4261 gccgcaagcg gtgcagagga tttcacgaga agagggtttc ataaacgtga ctggaaaaag
     4321 ggaacatttg tgtgggatga atgagtccgc attgtagcgc ggcggcgcgt gaatgcaaaa
     4381 agaaaagagg gtccgttgct gtgcggggaa accggtcaga aaaaaagtca acttttttta
     4441 aaaagtgttc ttgcattttc cgaaccaaag gatacattac tctcaaaaca acacgcgttg
     4501 tattgtcttc acgatgcgac agcatgttgt cgaactgagt cacacaaacg aaaggacaac
     4561 gcaatggcca ca
//
LOCUS       DAOSRB010000216         4571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17168_length_4571_cov_7.648140, whole genome shotgun sequence.
ACCESSION   DAOSRB010000216 DAOSRB010000000
VERSION     DAOSRB010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17168_length_4571_cov_7.648140"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /gene="carB"
                     /locus_tag="PLZ74_09710"
     CDS             complement(<1..1782)
                     /gene="carB"
                     /locus_tag="PLZ74_09710"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOR98638.1"
                     /translation="MLRNDIHKVLIIGSGPIIIGQACEFDYSGTQACKALRECGYKIV
                     LVNSNPATIMTDPVMADATYIEPLNAERIEQIIAQERPDALLPNLGGQTGLNLAMELS
                     KKGILDKYGVRVIGVNLDAIERGEDRQIFKDTMTKLGIETPQSEIAYSVENAETIASR
                     LGYPVVVRPAYTMGGTGGGLVYNVEELRAIVIRGLEASLTHQCLIEESVLGWEELEVE
                     VVRDAKNQMIAVCFIENIDPMGVHTGDSFCSAPMLTLSDEVRQRLQEYAFRIVESIGV
                     IGGTNVQFAHDPATGRIVIIEINPRTSRSSALASKATGFPIALISAKLASGMTLDEIP
                     YWRDGTLEKYTPSGDYIVLKFARWAFEKFRGVEDKLGTQMRAVGEVMSIGKTYKETFQ
                     KAIRALENGRHGLGFAKDFNRKTLPELLDLLRFPNSERQFILYEALRKGATDEQLFGL
                     TKIKPYFLGQMRELVELEERILQHKGGLPPDDLLIQAKRDGFSDKYLAKILGVLESVI
                     RTRRTALGIVETWHAVPVSGVENAYYYYSSYNGVRNEVPVSSNPKKVMILGGGPNRIG
                     QGIEFDYCCVHAAFAMRDAGYETIMVNC"
     gene            2213..3838
                     /locus_tag="PLZ74_09715"
     CDS             2213..3838
                     /locus_tag="PLZ74_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98639.1"
                     /translation="MKKTWTAMLILGLIGLRSGATILYRMTFDHTGAAGGEANAIYTA
                     GPGDLLDPHQSVIRYGTNPVPQIVASDGPQGGCALVTATQLPGSGQGPGYTAATTNSK
                     LYFVHGGHDRGLTLEAVFRATAWTANGWGGMVNYNNQHFLGFAWGTKNNPHIKFAVNN
                     IGVITNVPAESLCDDAWHHVAGVWTRDADGVTGRLEIFLDGVSVSSHAVPIPASAITI
                     PTGGWGFSAEGYTTPPRLRSFIGELDAAAVADDVRGPGRFLVAGCDADVFPDLYTEDF
                     ATGWSNGVAGFMGSGAAVPGRWTLRDGELSKWQVMETGTPQGLALAFTNGSFNSSWWP
                     TGMQTPFRFPVTGPVTVEFSARMRIACNAVSGKIWDARIHLMTRDQNGYGLSLKRSLD
                     SLAHGADNKVYARIIKYRGCTVALGTGSTSDWAEGDGASATDEVMVADADETGFIRLH
                     VRLTQEEPNGPVTVRLWHTESGVADTSLQNPDLTWMDDGSRFGAILNLRELNWLGVNA
                     EAQGSPNGPDAGVWFDDLRVERIRRSGMVMEIR"
     gene            4017..4093
                     /locus_tag="PLZ74_09720"
     tRNA            4017..4093
                     /locus_tag="PLZ74_09720"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4051..4053,aa:Pro,seq:ggg)
BASE COUNT          882 a         1274 c         1389 g         1026 t
ORIGIN      
        1 gcagttgacc atgatggtct cgtaaccggc atcgcgcatg gcgaaggccg cgtgcacgca
       61 gcaatagtcg aactcgatac cctgtccgat ccggttcggg ccgccgccga gaatcatgac
      121 tttcttgggg ttggaggaga cggggacctc gttgcgcacg ccgttgtagg acgagtagta
      181 gtagtaggcg ttttcgactc cgctgaccgg aacggcgtgc caagtctcga cgattccgag
      241 cgcggtccga cgggtccgga tcacgctctc gaggacgccg aggattttgg ccagatattt
      301 gtcggaaaac ccgtcccgct tggcttggat gagcaggtcg tccggcggca ggcctccttt
      361 gtgttggaga atccgttctt ccagctccac cagttcacgc atctgcccga gaaagtaagg
      421 cttgattttc gtgaggccga agagttgttc atccgtcgcg cctttgcgta acgcctcgta
      481 caggataaac tgacgttcgc tgttcgggaa tcgcagcaga tcgagcaact ccggcaaggt
      541 ttttctgttg aaatccttag cgaaaccgag tccgtgccgc ccgttctcca aggcgcggat
      601 ggccttctgg aaggtttcct tgtacgtttt accgatcgac atcacttcgc caaccgcacg
      661 catctgggtt cccagtttgt cctccactcc gcggaacttc tcaaaggccc agcgggcgaa
      721 tttcagcaca atgtagtcgc cggagggcgt gtacttctca agggtcccgt cccgccaata
      781 ggggatttcg tcaagagtca tgcctgaggc cagtttagcg gaaatgaggg cgatcgggaa
      841 tccggtggct ttggaagcga gtgccgagga gcgcgaagta cgcgggttga tctcaatgat
      901 cacgatgcgc ccggttgcgg gatcatgcgc aaactgcaca ttggtgccgc cgatgacacc
      961 gatggattcg acgatgcgga aagcgtactc ttgcagccgt tgccgtacct cgtcggagag
     1021 ggtcagcatt ggcgcggaac agaaggagtc gccagtgtgg acccccatgg gatcgatgtt
     1081 ctcgatgaaa catacggcga tcatctggtt tttcgcgtca cggaccacct cgacctcaag
     1141 ctcctcccag cccagcacgg actcctcgat caggcactgg tgggtcagcg aggcctccag
     1201 accgcggatg acaatggcgc gcaattcctc gacattgtaa acgagcccgc caccggtacc
     1261 gcccatggtg tatgccgggc gcaccaccac gggataaccc agacgcgacg cgatcgtctc
     1321 ggcgttctcg acggaatagg cgatctcgct ctgaggcgtt tcaataccca gtttggtcat
     1381 ggtgtccttg aaaatctggc ggtcttcgcc ccgctcgatc gcgtcgaggt tgacgccgat
     1441 gacacggacc ccgtatttgt ccagaatccc cttcttggaa agttccatgg ccaggttcag
     1501 gccggtctgg ccaccgagat tgggcaggag ggcgtcaggg cgttcttggg cgatgatctg
     1561 ttcgatccgt tcggcgttga gaggctcgat ataggtggca tccgccatca ccgggtccgt
     1621 catgatcgtg gccgggttcg agtttaccag gacgattttg tatccgcatt cgcgtagcgc
     1681 cttacatgcc tgggtgccag aataatcgaa ttcacacgct tgaccgatga tgatcggacc
     1741 agagccgata ataagaacct tgtggatatc gttgcgcagc atagggtggg cctgaccttt
     1801 cggacttgat tcggacgtct ccagcccgcg ccgacgcgcg gggagccgcc tcaaaacacg
     1861 ctcatgatga tgccagtttc gcccgcgctc gtcgagtcaa agctcgcccg ctgagggatt
     1921 cgggttcctg gtttggggtt tcttgttttc ggaaccccct tctcccgcgt ctgtaacccg
     1981 gctgtttgtt gcgctaaacg tgctgcaacc agtaggaacg tccccgaacc gtccctattg
     2041 attgatgacc gaaagagatt catcgcgttc gttgttatcc ccaaaaccgg actttcgaca
     2101 ttgactcgtt tcgtgcccct ttgcataatg actttcatta ctgaaagcaa tgtgttcccg
     2161 gtttactcca atgggggaga agtggcgttt ccaaccgcaa agggagggtg tcatgaaaaa
     2221 aacttggacg gctatgttga ttctcggttt gatcggttta cgttccggtg caaccattct
     2281 ctatcggatg actttcgatc acacgggtgc ggcaggcgga gaagccaacg cgatttacac
     2341 ggcaggaccg ggtgatttgc tcgacccgca ccagtcggtc atacgctatg gcaccaaccc
     2401 cgtaccgcaa atcgttgcga gcgacggtcc gcagggcggt tgcgcgctgg tcacggccac
     2461 gcagcttccg ggaagcggac aagggccggg atacaccgca gccacaacca acagcaagct
     2521 ttactttgtt catgggggcc acgaccgcgg gttgactctg gaggcggttt tccgggccac
     2581 agcctggacc gcgaatggtt ggggcggcat ggtgaactat aacaaccagc atttcctcgg
     2641 tttcgcttgg ggcacgaaaa ataatccgca catcaagttc gcggtgaata acattggtgt
     2701 cattacgaac gtcccggccg aatctctgtg cgatgacgca tggcaccatg tggcgggtgt
     2761 ctggacacgg gatgcggacg gcgtgaccgg gcggctcgaa atttttctcg acggtgtttc
     2821 ggtctcgagc cacgcggtgc cgattcccgc ttctgccatc accattccca ccggcgggtg
     2881 gggtttttcc gccgagggat acacgactcc gccgcgtttg cgctctttca tcggggagtt
     2941 ggatgccgcg gcagtggcgg atgacgtgcg cggtccggga cggtttttgg tcgcggggtg
     3001 cgatgcggac gtgttcccgg acctctatac tgaggatttc gcgactggct ggtctaacgg
     3061 ggtcgccgga tttatgggga gtggcgcagc cgttccgggc aggtggactt tgcgggatgg
     3121 ggaactcagc aagtggcagg tgatggaaac cggtacgcca caagggctgg cgctcgcttt
     3181 cacaaacggg agttttaaca gcagttggtg gccaacagga atgcagacgc ccttccggtt
     3241 cccggtgaca gggccggtga ccgtggagtt cagtgcgcgc atgcgaatcg cctgcaatgc
     3301 agtgtctggt aaaatctggg atgccagaat ccatttgatg acccgtgacc aaaacggata
     3361 cggcctgtcc ctcaagcgga gtctggactc cctcgcgcat ggcgcggaca acaaagtcta
     3421 cgcccggatc attaaataca gggggtgtac agtggcattg ggtacgggat cgacttcgga
     3481 ctgggccgaa ggtgatggtg cgagtgccac cgatgaggtc atggtggcag atgccgacga
     3541 gacgggtttc atccgcctgc atgtgcgtct gacgcaggag gagccgaacg gaccggtgac
     3601 cgtccggctc tggcataccg agagtggagt ggcggacacc tcactgcaaa atcccgacct
     3661 gacttggatg gatgacggat cgcgtttcgg cgcgattctg aatctccgcg aacttaactg
     3721 gcttggcgtc aatgccgagg cgcagggttc accgaatggc cccgatgcgg gcgtttggtt
     3781 tgacgacctg cgggtggaac gaatccggcg ttccggcatg gtgatggaaa tccggtgact
     3841 cttttcggtg agccgttggc acgatgcggc tgcggcgcgt ccgtctgggt gcgatccgat
     3901 gacgtttcgt gctgtgcggc gtatcggttc ggtgttgcgg tttttcgttt ccgggcgatt
     3961 ccgtttgacg tgccgtcttc cgacttggta cagtctgctc tgcactttgt atttttcggg
     4021 gcgtagcgca gcctggtagc gcgtttgctt ggggtgcaaa aggtctcggg ttcaaatccc
     4081 gacgccccga ccatttttca cctcgttttt atggataaac cccaataaaa gcggggtttt
     4141 ttgtttgtgc agcatagtta ccccttggct tgcaacaatc tgaaacaagc tgatttatag
     4201 gggtcattta cacacacaaa cacacacaca aagccaaaag cccgaaagcg gtttcacgct
     4261 tgcccgcttg gttatagtgg cattttagac ggtcaaaagc cgcttgtttg ggcttttcgg
     4321 aagccaaaac agcccccagc cccagaccgg aagccccagc tttgcgcccc ttgcctgaaa
     4381 atcccgctct aggacacggt ttcccgcatc cgccgcccgt gggggtgtaa tgacgtgggt
     4441 ctgccgccct cgcgccgcct aacgcattct agccgctatt ccggcgcaac aaaaaagccc
     4501 gcctgcattc ctgcatgacg ggcaccgggg tttgatcgag acgcggccta gtccgcatcc
     4561 tcgagttgcc g
//
LOCUS       DAOSRB010000217         4563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17191_length_4563_cov_8.173248, whole genome shotgun sequence.
ACCESSION   DAOSRB010000217 DAOSRB010000000
VERSION     DAOSRB010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17191_length_4563_cov_8.173248"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1080)
                     /locus_tag="PLZ74_09725"
     CDS             complement(268..1080)
                     /locus_tag="PLZ74_09725"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOR98640.1"
                     /translation="MKRILILIFLSVSVLPGMASEEVWSGTKVISEPVKIVGARVRVL
                     PGAKIIFAGEGRIHMRDGSFTASRAEFLSESVLTNDFRMRFTECGFEARHCRFTGLRT
                     EKPDKRSFIYGFAFAISNGKMTLDHNTFVDCSAFMSAAVSHGMFTRNLFVRCAGGLAL
                     LNATQCRVEGNEFFDSPHEAMKLNTTRLSEIFRNRFTRCHIGMAFYAAKENRITGNAF
                     FASPIAFNLMHSGPDNVFSGNRYENVRQKYFNQGKSDGNRFFDDTPQPPTLK"
     gene            complement(1130..3904)
                     /locus_tag="PLZ74_09730"
     CDS             complement(1130..3904)
                     /locus_tag="PLZ74_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOR98641.1"
                     /translation="MKCMIKQMMTVSVAMLSATVVAMPTLQPCVKEYRALESAAFNTA
                     ELPVFHQDQRQCVIGAAETFVKGERKIWDGKSAAANGVYLAIADSACGKKLIEAFSLD
                     VPAKPQGYAIKAEGRRVAIVGHDDVGALYGAVTFAQMSRNGRVEAAYVRDWPDVLYRG
                     SISFGRGLWKFRYGEKDEKGYSDAIKAGLDMMLRAKMNLFSDLFWLEEGMPDSTFPFF
                     RELGRYAAERGIWCNYYATTGVYIRRTAPKGMKFDDWKCVKLHCPWNDSYYCWADDAL
                     TEAAANRTVDFLVKAGMEKAVVNIHPVDGGSWQDPEEWSKRCEKCRAKWNDHERWKAS
                     INQFNIWIRVFRKRLPGAVVGSCIYPYQFNALMKPESERTAKWKESMPEYWDKLDKGL
                     ADKEFYFSSWITTPDVIKTVRELVPERPFHLSDTYPLTAGIFASYPRKIGSSCEGGRN
                     FATTQGTECYLQIETLLLVNEHLWDLHAPGTENYDGLTYYDGLKDHVAPDVIMKDSLG
                     RICRTFWGDVLAPYMQTVLSSGVMPEYLRDPAAKVKYWNKVRRDPDFDPTQPEKSKNS
                     GSLKYPPIIDTPELMLAQVEAAERCVHTLLEAEKRLDGLDRYKRKHFMRFLKYAPYWL
                     ATARTHHVVRLAGAALYDGDNAKALGILKEGRVRIEKDFDLAETTFKRLEEEADTLTD
                     PRRPRDASQTWKFDRKAATTVLDQTEASARITLEPRKIGAVVKLGVHRGGGDVCIKEY
                     FDQFENVKAEYFDSISLAELNRFDCVFLTQKQYEKDAFFTNVKTYVEKGGGGVFLEGE
                     LCGNVRFDVKTPFPEIVKTSPARVENFKRKMKFADGREGETMYVDYFRLTPGAKGEVL
                     AYGPNSGDVLAVRGVAGLGKVFFSGTLNIGSVADSYATRACPLFGANAEFAREAVEYF
                     TNVRLKEKDE"
     gene            complement(3969..4514)
                     /locus_tag="PLZ74_09735"
     CDS             complement(3969..4514)
                     /locus_tag="PLZ74_09735"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOR98642.1"
                     /translation="MNLTERTLGVKRVFEGRALTIDVAEIELPNGRRSTREIVRHRGA
                     AVILGQRPDGRFVLIRQYRRAVEGVLLEMVAGGLEEGESPETCARREMEEESGYPVET
                     LEKIGVIVPCPGYSEERLHLFYARLSQEPRPLRPDFDENLAPVVMTAEEIDAALDSGE
                     LIDGKSIALWLMWRRSTRRHS"
BASE COUNT          857 a         1452 c         1284 g          970 t
ORIGIN      
        1 gcgaccgtgc gtcataaaga cccagttttg gtcgcattta ctttgccaag tgacccctgc
       61 cgccgtgatc gaagacgcat ccccggggcg ttgatcgttg gggggggagg tttcattcgg
      121 gaacgcgggt gtccccgtcc gccgctatgc gccatcggcg cggggccgga ccgccgaaat
      181 cgaggacgag gacggggacg ggggaaacga tcccgatgcc atcaggccgg gagggcaaac
      241 gtctgcttgc ccggacgagc ccatttctta cttgagcgtg ggcggctgcg gcgtgtcgtc
      301 gaagaaacgg ttgccgtctg atttcccctg attgaaatac ttttggcgga cgttctcata
      361 ccggttgccg gagaacacgt tgtcaggccc cgaatgcatc agattaaagg cgatgggcga
      421 cgcgaagaac gcgttgcccg tgatccggtt ttccttcgcc gcatagaatg ccatgccgat
      481 atggcaacgt gtgaagcggt ttcggaaaat ctccgaaagg cgcgttgtgt tgagtttcat
      541 cgcctcgtgc ggggaatcga aaaactcatt gccctccaca cggcactggg tggcgttcag
      601 aagcgcgagt ccgcccgcgc accgcacgaa caggttccgc gtgaacatgc cgtgcgaaac
      661 cgccgcggac atgaaggccg aacagtccac gaacgtgttg tgatcaagcg tcatcttccc
      721 gttcgaaatc gcgaatgcga aaccgtagat gaagctgcgc ttgtccggtt tctcggttct
      781 gagccccgtg aacctgcaat ggcgcgcctc gaaaccgcac tcggtgaaac gcattcggaa
      841 atcgttcgtg agcacgcttt cagacaaaaa ttccgcccgc gaagccgtga acgaaccgtc
      901 ccgcatgtgg atccgtcctt cgcccgcaaa gatgatcttc gcgcccggca acacgcggac
      961 gcgtgccccg acgatcttca ccggctcgga tatgactttc gtcccggacc acacctcctc
     1021 cgatgccata cccggcaaga cagacacaga caggaaaatc aggatcagta ttcttttcat
     1081 gaacgctcac acttgttgtc accgttgtcg gcaatccgtt ttttggccgt cattcgtcct
     1141 tttcctttag gcgcacgttc gtgaaatact ccaccgcttc gcgggcgaac tcggcgttgg
     1201 ctccgaagag cggacacgcg cgcgtcgcgt agctgtcggc gacggaaccg atgttgagcg
     1261 taccggaaaa gaataccttg ccgagaccgg cgacgccgcg gacggcgagg acgtccccgc
     1321 tgttcgggcc gtacgcgagc acctcgcctt tcgcgccagg cgtcaggcgg aagtagtcca
     1381 cgtacatcgt ttccccttcg cgaccgtccg cgaacttcat tttccgcttg aaattctcca
     1441 cacgcgccgg tgaggtcttc acgatctccg ggaacggcgt cttaacgtcg aatcggacgt
     1501 tgccgcaaag ttcgccctcg agaaacacgc cgccaccgcc cttctccaca taagtcttca
     1561 cgttcgtgaa gaacgcatcc ttctcatact gcttttgcgt gaggaacacg cagtcgaacc
     1621 gattgagttc cgcaagcgaa atggagtcga aatactccgc ctttacgttt tcgaactggt
     1681 cgaaatattc ctttatgcag acatcgccgc caccccggtg gacaccaagt ttcacgacag
     1741 cgccgatttt acgcggttcg agtgttatgc gtgccgaggc ctcggtctgg tcgagcaccg
     1801 tcgtcgcggc cttccggtcg aacttccagg tttgggaggc atctctcggc cgccgcggat
     1861 cagtgagcgt gtccgcttcc tcctcgagcc gtttgaatgt cgtttcagcg aggtcgaagt
     1921 ccttttcgat ccgaacacgg ccttccttga ggattcccaa cgccttcgcg ttgtcgccgt
     1981 cataaagcgc ggcgccggcg agacggacca catgatgcgt gcgtgcggtc gcgagccaat
     2041 acggcgcata tttcaaaaag cgcatgaagt gcttgcgttt gtagcggtcg agtccgtcga
     2101 gacgcttttc cgcctccaga agcgtatgga cacatctttc ggccgcttcg acctgcgcga
     2161 gcatcaactc gggggtgtcg atgatcggcg gatacttgag cgagccggag ttcttcgact
     2221 tctccggttg cgtggggtcg aaatcgggat cgcgccggac cttgttccag tatttcacct
     2281 tcgccgccgg atcgcgcagg tactccggca tcacgccgga tgacaaaacc gtctgcatgt
     2341 acggcgcgag cacgtcgccc cagaacgtgc ggcagatgcg gccaaggctg tctttcatga
     2401 tcacatcggg tgcgacatga tccttcaggc catcgtaata ggtgaggccg tcgtagtttt
     2461 cggtgcccgg cgcatggaga tcccagaggt gctcgttcac gagcagcagc gtttcgattt
     2521 gcaggtaaca ctcggttccc tgcgtggtcg cgaaattccg ccccccctcg caactcgacc
     2581 cgatcttgcg cgggtaggac gcgaagatgc ctgcggtgag cggataggta tcgcttagat
     2641 ggaacggacg ctctggcacg agttcccgta ccgttttgat cacgtccggt gtggtgatcc
     2701 aacttgagaa gtagaactcc ttgtcggcaa gccccttgtc gagcttgtcc cagtattccg
     2761 gcatggactc cttccacttc gcggtacgtt cgctctccgg cttcatcagc gcgttgaact
     2821 ggtacgggta gatgcacgag ccgacgaccg cgccaggaag tcgcttgcgg aaaaccctta
     2881 tccagatgtt gaactggttt atgctcgcct tccagcgctc gtggtcgttc catttcgcac
     2941 gacatttttc acagcgtttg ctccactcct ccgggtcctg ccagctgccg ccgtctacgg
     3001 gatggatgtt gacgaccgct ttttccatgc cggccttgac gaggaaatcg accgtccggt
     3061 tcgccgcggc ctcggtaagc gcgtcgtccg cccagcagta ataggagtca ttccacgggc
     3121 aatggagctt cacgcatttc cagtcatcga acttcatccc cttcggcgcg gtacggcgga
     3181 tgtacacccc ggttgttgcg tagtaattgc accagatgcc gcgttcggcg gcgtaacgtc
     3241 cgagttcgcg gaagaacggg aaggtgctgt cgggcatgcc ttcctcaagc cagaagaggt
     3301 cgctgaacag gttcatcttc gcgcggagca tcatgtcgag gcccgccttg atcgcgtcgg
     3361 aatatccctt ttcgtccttc tcgccgtagc ggaacttcca gagtccgcgc ccaaaggaaa
     3421 tcgagccgcg gtacagcaca tccggccagt cccgcacgta tgccgcctcc acccggccgt
     3481 tccgggacat ctgcgcgaac gtcaccgcgc cgtaaagcgc gccgacatcg tcatgcccga
     3541 caatcgcgac acgcctgcct tccgccttga tcgcgtaccc ctgcggtttc gccgggacgt
     3601 cgagcgaaaa cgcctcgatc agttttttgc cgcaggcact gtccgcgatc gcgaggtaga
     3661 cgccgtttgc cgcagcgctt tttccgtccc agattttccg ttcgccttta acgaacgttt
     3721 cagccgcgcc gatcacgcac tgccgctgat cctgatgaaa aaccggcagt tccgccgtgt
     3781 tgaacgccgc gctttcgagc gccctgtatt ctttcacgca cggttgtagt gtcggcatgg
     3841 ccacgaccgt cgccgacaac atggcgaccg acaccgtcat catctgcttt atcatgcatt
     3901 tcatatcggt ttctcagctt ctacggacga acgcggtgcg gaatccaagc aattccgcat
     3961 gagtagggct aactgtgccg ccttgtgctt cgtcgccaca tcagccagag ggcgatgctc
     4021 ttgccgtcga tcaactcgcc agagtcgagg gccgcgtcga tctcctccgc cgtcatcacc
     4081 acgggcgcga ggttctcgtc gaaatccgga cgcagcggcc ggggctcctg cgacagacgc
     4141 gcgtagaaga gatgcaggcg ctcctctgaa tagccggggc acggcacgat cacgccgatc
     4201 ttttccagcg tctccaccgg atatccgctc tcctcctcca tttcacggcg cgcgcacgtt
     4261 tccggagact ccccctcttc cagccctccg gccaccatct ccaggagaac gccctccacc
     4321 gcgcgccggt attggcggat caacacgaac cggccgtccg gccgctggcc gagaatcacc
     4381 gcagccccgc ggtgccgcac gatctcgcgc gttgagcgcc gcccgttcgg cagttcaatc
     4441 tccgccacgt cgatcgtcag cgcccgtccc tcgaaaaccc gtttcacgcc cagtgtccgt
     4501 tctgtcagat tcatatccgc ttaagtgtcg cagaaacaac cgcaccggac aaccccgaaa
     4561 aaa
//
LOCUS       DAOSRB010000218         4545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17272_length_4545_cov_6.424722, whole genome shotgun sequence.
ACCESSION   DAOSRB010000218 DAOSRB010000000
VERSION     DAOSRB010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17272_length_4545_cov_6.424722"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..502)
                     /locus_tag="PLZ74_09740"
     CDS             complement(2..502)
                     /locus_tag="PLZ74_09740"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOR98643.1"
                     /translation="MTMIELGIVIGIVSILMALVLGLARHVNATVKIRRAQTELGEWH
                     DTLNRWRLQFGEYPHARIDTTRDDSVDLLDSIDLTEPSRFPLTILTNQCEIIYPDRTV
                     TFRSFLTSVVSTVDPWGTPYIYIPSDNAQAYTLFSCGPDRKSADIPTGAASTPDPTLD
                     DIHFER"
     gene            complement(860..2578)
                     /locus_tag="PLZ74_09745"
     CDS             complement(860..2578)
                     /locus_tag="PLZ74_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOR98644.1"
                     /translation="MPDIRYASDELLAEVTQHLVDRGLLTPDKLAELQNRSALTLQGI
                     DQILIQEGIVNETDLLKAFSSISGIPYHTIGDFRIEQEAIEKVTAKVALRHRIVPLTY
                     QNGVLLLAASRVPNLTTVDGLRMVLDAGIDFVLCPESDIAMSLTHFYGLGADTIDQLI
                     AEAEAMPQEQEDDDIAVQTQETGMVRFINLIIAEAIRMDCTDIHIEPFENTLRLRYRI
                     DGILQEIPLPKGVDKLRNAVSSAVKIMANMDIAEHRKPHDGRIKVRCGNSDYDLRVSV
                     LPTGWGETCCLRILSRGTIKIDLDTLGLSPYQLPKIAKLTELPHGVILMTGPTGSGKT
                     TTLYALLARLNKTGTKIITVEDPVEYQMKGISQVQTHSKIGLTFANVLRSILRHDPDV
                     ILIGEIRDSETADIAVRSSLTGHLVLSTLHTNDAPSAVTRLIDMGVEPYLISSCVEGI
                     VAQRLVRRVCRNCNEPYEVPETIRNEIAMTYPDRIGEATFIHGHGCPTCGFTGYRGRS
                     AINEVMIMSDPLRAMVVTREPANIIKELAMKEGMVTLRQDGWLRVVEGRTSVEEVLRV
                     AGRMEE"
     gene            complement(2708..3532)
                     /locus_tag="PLZ74_09750"
     CDS             complement(2708..3532)
                     /locus_tag="PLZ74_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98645.1"
                     /translation="MDTLWKGIMRLNAKAFCLLAMLFFAGVAGFCFWQYRHPAEPIRD
                     HDSGPLPEPPAVVTIGLLDFVTNQLTAETLVMPVDPFRPTIEAIFTNEVERAAFLKAL
                     KAAQAAASGISAATGGASTAAKAKENPFAHLRKKAPAPGGLVGPDGTPLIIPKLTFVG
                     FFERPDGKKAALFHDSTEQSTAFYDSGKTVHGVEILSANIREVEIRFPDGTTRKLAIG
                     SSVELAPEPAKVKPKKSAKPSAKTGAAPQAGAKAAAGKQAAAKAAAARPAKPQAFK"
     gene            complement(3668..4387)
                     /locus_tag="PLZ74_09755"
     CDS             complement(3668..4387)
                     /locus_tag="PLZ74_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98646.1"
                     /translation="MQVVSLLLLIIVCAGLTVRFVVNPQRELAANNRQVREQLHRSQY
                     ASLSTASMQTLQAHESTTLSALSNEWAHLAERLATFPDSLAMPGSEVNRIDYKVELYA
                     IRERLQTKSGELKIQLLPTELGLDETVASGDIIRVRMLQLKAVERLADLALDRQIQRL
                     VEIYPLQPVEHRDAKGHRTFEEYPVRLECDVDFEHLFTFFQSVFEKDQVFVFRNLRIE
                     SGPTFDAKLRVKAILSALLFE"
BASE COUNT          846 a         1201 c         1515 g          983 t
ORIGIN      
        1 gtcaccgttc aaagtggatg tcgtcgaggg tggggtcggg cgttgacgcg gctccggtcg
       61 ggatgtcggc ggatttgcga tccgggccgc aggaaaaaag cgtgtaggct tgggcgttgt
      121 cggaagggat gtagatatag ggcgtgcccc acggatcgac tgtgctgaca acggaggtga
      181 ggaacgagcg gaaggtcacg gttctgtctg ggtaaatgat ctcacactgg ttggtcagga
      241 tggtgagcgg gaaacgcgag ggctccgtca aatcgatgga atcgagtaaa tcgacggaat
      301 cgtctcgggt ggtgtcgatg cgcgcgtggg gatattcgcc gaactggagg cgccagcggt
      361 tcagcgtgtc gtgccattcg cccagctccg tctgtgcgcg gcggattttg accgtggcgt
      421 tcacatggcg cgcgaggccg agcacgaggg ccatgaggat tgagacgatg cctatgacga
      481 tgcccagctc gatcatcgtc atgccgccgc agcgccggca tgaagtagta ggtaataggt
      541 aataggtaat aagtggttgg ggggggatgg gcaaaggtct tgctggatga cgccgcatcc
      601 gcgtcatcga cacatcacaa gcgtggctgg aacaccgcac gaccttctac ctcttacctc
      661 tcacttctta cctcacccct tacctactta cctactacct attacctctc acctcttacc
      721 tcttacctct tacctactac ctattacctc tcacctctta cctattacct actacctatt
      781 acctctcacc tctcacctct cacctattac ctctcacctc ttacctatta cctcttacct
      841 attacttctt acctccctct tattcttcca tgcgtccggc gacgcggaga acctcctcga
      901 cggaggtgcg gccctcgacc acgcgcagcc agccgtcctg acgcagggtc accatgccct
      961 ctttcatggc cagttctttg ataatgttgg ccggttcgcg cgtgacgacc atggcgcgca
     1021 gcgggtcgga catgatcatg acctcgttga tggcactgcg gccgcggtac ccggtgaagc
     1081 cgcatgtcgg gcagccgtga ccgtgaatga acgtcgcctc gccgatacgg tcggggtagg
     1141 tcatggcgat ctcgttacgg atcgtttccg ggacctcgta gggctcgttg cagttgcggc
     1201 agacgcggcg caccagacgc tgggccacga tgccctcaac gcaggaggag atgagatagg
     1261 gctcgactcc catgtcgatc aggcgggtga ccgcactggg cgcgtcgttg gtgtggagcg
     1321 tgctcagcac cagatggccg gtgagcgagg agcgcaccgc gatgtcggcg gtttccgaat
     1381 cgcggatttc gccgatcagg atcacgtccg ggtcgtggcg caggatcgag cgcagcacgt
     1441 tggcgaaggt cagcccgatc ttgctgtggg tctggacttg gctgatgccc ttcatctggt
     1501 attcgaccgg atcctcgacc gtgatgattt tggtgccggt cttgttgagg cgggcgagca
     1561 gcgcatacag cgtggtggtt ttaccggagc cggtggggcc ggtcatgagg atcacgccgt
     1621 ggggcagttc ggtgagtttg gcgatcttgg gaagctggta gggcgagagg ccgagggtgt
     1681 cgaggtcgat tttgatggtg ccacggctaa ggatacgcag acagcaggtc tcgccccagc
     1741 cggttggcag gactgagaca cgcaggtcat agtcgctgtt gccgcaacgc accttgatgc
     1801 ggccgtcgtg aggtttgcgg tgctcggcga tgtccatgtt cgccatgatc ttcacggcgg
     1861 agctgaccgc gttgcgcagt ttgtccacgc ctttcggcag cggaatctct tgcaggatgc
     1921 cgtcgatgcg gtaacgcagg cgcagggtat tctcgaaggg ctcgatgtgg atatcggtgc
     1981 agtccatccg gatcgcctct gcgatgatca ggttgatgaa gcgcaccatg ccggtctctt
     2041 gggtctggac cgcgatatcg tcgtcttctt gctcttgcgg catcgcctcg gcttcggcga
     2101 tgagctggtc gatggtgtcc gcgccaagac cgtagaaatg ggtcagcgac atggcgatat
     2161 cgctttccgg gcagagcacg aaatcgattc cggcgtcgag caccatgcgc aggccatcca
     2221 ctgtggtcag gttgggtaca cggctggccg cgagcagcag gaccccgttc tgataagtga
     2281 gcgggacgat gcggtggcgc aaggcgacct tggccgtgac tttttcgatc gcttcctgct
     2341 caatcctgaa atcgccgatc gtgtgatagg ggatgccgct gatggaggaa aaggccttta
     2401 ggagatcggt ctcgttaacg atcccctcct ggatcaaaat ctggtcgatg ccctgcagcg
     2461 tcagtgcgga gcggttctga agttccgcca gtttgtcggg cgtgagcaag ccacgatcaa
     2521 ccagatgctg ggtcacttca gcgagcaatt cgtcagaggc gtagcggatg tcaggcatag
     2581 gtggggaaag gggttagggt tttaacacag aggcacagag atagggagga aggaagaagg
     2641 agattatcag cgcagagacg caaaggcgca aggatgcgga gagggaagga atctgggggt
     2701 gatgcggtca tttgaaggct tggggtttgg cgggacgggc tgcggcagcc ttggcggcgg
     2761 cctgtttccc ggcggcggct ttggcgccag cctggggtgc ggcacccgtt ttggcactgg
     2821 gcttggccga ttttttgggt ttcaccttgg cgggctcggg cgcgagttcc acggaggagc
     2881 cgatcgccag cttgcgtgtg gtgccgtcgg ggaaacggat ctccacctcg cggatgttgg
     2941 cggagaggat ttccacgcca tggactgttt tgccagagtc atagaaagcc gtgctctgtt
     3001 cggtggagtc atggaaaagg gcggctttct tgccgtccgg ccgctcgaaa aaccccacga
     3061 aggtaagttt ggggatgatc aggggcgtgc cgtccggtcc aaccagtccg ccgggtgcgg
     3121 gcgctttttt ccgaaggtgc gcgaaagggt tctctttggc ctttgcggct gtgctcgcgc
     3181 cgccggtcgc cgccgagatg ccggatgccg cggcttgggc agccttgagg gccttgagga
     3241 atgcggcacg ctcgacctca ttggtgaaga tcgcctcgat ggtggggcgg aaggggtcga
     3301 ccggcatgac gagtgtttcg gcggtcagtt ggttggtgac gaaatcgagc agtccgatgg
     3361 tcaccacggc gggcggttcc gggagggggc ccgaatcgtg atcccggatc ggttcagccg
     3421 ggtggcggta ctgccagaag cagaagccag ccacacccgc aaagaaaagc atggccagca
     3481 gacagaacgc cttcgcgttg agtcgcatga ttcctttcca gagcgtgtcc ataaaaatcc
     3541 tttagctacc agttgtttct cacacgaagg cacgaaggcc acgaaggaga atggggtgcc
     3601 atgaaaatct ttgtgtcctt tgtgcctttg tgtgaggtta aaatcgggtt gcgggtgccg
     3661 ccccgcgtca ttcaaacagg agcgcggaga gaatggcctt gacgcgaagc ttggcgtcaa
     3721 aggtcggtcc ggactcgatg cgcaggttgc ggaaaacgaa gacctgatcc ttttcaaaaa
     3781 ccgactggaa gaaggtgaag agatgctcga agtccacatc gcactccaga cgcaccggat
     3841 actcttcgaa cgtgcggtgc cccttggcgt cacggtgctc gaccggctgg agcgggtaga
     3901 tctccaccaa gcgctggatt tgacggtcga gcgcgaggtc ggcgagccgt tcgaccgctt
     3961 tgagttggag catccgcaca cgaatgatgt cgccgctggc cacggtctca tccagaccga
     4021 gttcggtggg cagcagttgg attttcagtt cgccggattt ggtctgcaga cgctcgcgga
     4081 tcgcgtacaa ctcgaccttg tagtcgatgc ggttcacctc ggatccgggc atggcaagac
     4141 tgtccgggaa agtggcgagg cgttcggcca gatgcgccca ttcgttcgag agtgcgctca
     4201 gggttgtcga ttcatgcgcc tgaagcgttt gcatgctggc ggtcgagagg gacgcgtatt
     4261 ggctgcgatg gagctgttca cgcacctgcc ggttgtttgc cgcgagttcg cgctgaggat
     4321 tcaccacaaa gcggaccgtc agccccgcgc atacgatgat gagcaacagg agggacacca
     4381 cctgcatacg ctcggttgtg agcaggcgga agaccgatgg aagatgtagg gtagggaagg
     4441 acatggtttc gggttttcaa ggtttcaggg tttcgggttt cagggtttcg gggttcatcc
     4501 aaatcggggt cggggtcggg atcgctatcg ggatcgatca tttaa
//
LOCUS       DAOSRB010000219         4522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17381_length_4522_cov_6.110589, whole genome shotgun sequence.
ACCESSION   DAOSRB010000219 DAOSRB010000000
VERSION     DAOSRB010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17381_length_4522_cov_6.110589"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PLZ74_09760"
     CDS             <1..1249
                     /locus_tag="PLZ74_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOR98647.1"
                     /translation="MAALTARGAGQDGVFQVTAYGAVPDGVTDNTAAIQKAIDACAAA
                     GGGTVRVAGGAFMTYTLYPRSNTRLEIEAGASLEGGPDPLRYPEFVPSPHWRVEYSLR
                     RNKRAMFYAVAQTNLTICGRGTINGHAELFTEPSGSGHWWKRKHDQLITGRNIFLVGC
                     SDVTLEGFSIYNPAGWSMWLLDCDRVNIRGLKIDMNLKFPNGDGIHIGSCRDVTVSDC
                     IVRSSDDSIILRAYQHQLNEPKACERVVVANCVLESRSSAIRIGWTHDYEIKDCRFSN
                     LVIRDSITGISIVMPAVAHVPNDPPRGPGVPPLPEGDAVRPFGVENVHFSDISMNCRD
                     PIRVSFAPDTKVSRLRNITFSNMTIRSPEYPSFVLRKGDRVSDILLSNVRFELLPGGK
                     NTFNIRGVERLTLDRVTFIDSSE"
     gene            1273..4329
                     /locus_tag="PLZ74_09765"
     CDS             1273..4329
                     /locus_tag="PLZ74_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98648.1"
                     /translation="MRAGRFRRGGWTALLMGVPVWAFCAAGDIVRPMGSFDRAEFTRP
                     EDAERVRRSGNLAGTSEPGKNCYIKGWAEYDIEVPQSDWYELIVPGQAWGVEYRFDGD
                     VAVSAGSGKIGNFWLEAGRRTLRVQRYHWTGFQRIESFTLRPAEKKPAKRLRLWMPGE
                     NRIVRTGEVLPLRLQAGGPDGQPGWVTILLSDPKTQEIISQAEVAVPVGERPAEQPVT
                     LACPREGLFELRVSADGRRIDNLDVPVRPVVVVDTRPAARGGELRKALIAEIDCAVAA
                     PQFSGTGGANVVRSEAGVYRESGDAGFLRAQHNKQECGWFAYRLGVPEAQAPYVLEAD
                     YPDDAFRTFCIAIREAAPDAYPIAGGVDSGECYALTRRMQTHALLFWPRTRDLRVLFV
                     TAHNGRRGAAVSKIRLYRVEGDLPLPSVPPRGGRSFGNWYEEGSNFMAVYGAPDRSPR
                     GFLIGADRWARSIASVGGDTLVSTVAVYQMAMYPSRYNIDFSEPHSFDVVRVLLLKCE
                     KYGLGFIGEFHPECRELDRLADPTGQITPKPNGLISKDGKGGKDWSAIPRFHPLHPVD
                     REWYLGMIGEFVDRYKDSPAFRGVSLRLMGWANPGLNNFHSLDWGYDDLTVSLFEKET
                     GLSTGARADDPKRFRARYDWLMANARGAWTAWRCRKIMELHAAIRDRVRRARPDLTVY
                     LNNFGFGEEAGIAPKLLHALDGVVLVNAGHAYGRRAYTYEGPLADSKRRDKLLDPAAL
                     LALRNTEGKTAFLFGAGYFEATERVVPPASLGFPEDTKRTWMSGVVNPAGRHYLERYA
                     LALAEADAQYLSDGGNAYTLGQPLLTDFLREYRALPALPFRPREDGRDPVAVWELPGQ
                     ERFLFYAVNRERFPVPVAIRLTGTSEVRRFSSAKPQRLEDGVLRLTLQPYELAAFEAG
                     GGAALAGVTEQVPPEELARAEKQTAWMEALAVEARADRVGGQLSEAQAAQVVEAAAAS
                     RAALACRHVWQARTLLERSGLIQVYRILKRFPPALYDMGDESAGVR"
BASE COUNT          764 a         1370 c         1583 g          805 t
ORIGIN      
        1 gatggcggcc ctgacggcac gcggtgcggg acaggacggg gtgtttcaag tcacggcgta
       61 cggggcggtg ccggacgggg tgacggacaa cacggcggcg atccagaagg cgattgacgc
      121 ctgcgcggcc gcaggcggcg gaacggtgcg tgtggcgggt ggagcgttca tgacgtacac
      181 cctctatccg cgctcgaaca cccggctgga gatcgaggcc ggcgcttcgc tggagggcgg
      241 ccccgacccg ttgcgctatc cggagttcgt tccgtctccc cactggcggg tcgagtacag
      301 cctgcgccgg aacaagcgtg cgatgttcta cgcggtcgcc cagaccaacc tcacgatctg
      361 cgggcgcggc acaatcaacg gccatgcgga actcttcacc gagccgtccg gcagcgggca
      421 ctggtggaaa cggaaacacg atcagctgat cacgggacgg aacatttttc tggtgggatg
      481 ctcggacgtg acgctggagg gcttctcgat ctacaacccg gcgggctgga gcatgtggct
      541 tctggattgt gatcgggtca acatccgagg gctcaagatc gatatgaacc tgaagttccc
      601 gaacggcgac gggatccaca tcggctcctg ccgggatgtc acggtcagcg actgtatcgt
      661 ccgcagcagc gacgactcca tcatcctgcg cgcctaccag caccagttga acgagcccaa
      721 ggcctgcgag cgggtcgttg tggccaactg cgtgctggag agcaggtcct cggcgatccg
      781 catcgggtgg acccacgact atgagatcaa agactgccgg ttcagcaatc tggtgatccg
      841 ggactcgatc acagggatca gcatcgtgat gcctgccgtg gcgcacgtcc cgaacgatcc
      901 gccgcgcggg ccgggtgttc cgcctctgcc ggaaggggat gccgtgcggc ctttcggcgt
      961 tgagaacgtg catttcagcg atatcagcat gaactgccgg gacccgatcc gggtcagctt
     1021 cgcccctgac acaaaggtca gccgccttcg caacatcacg ttttcaaaca tgacgatccg
     1081 ttcgcccgag tatccctctt ttgtgctacg caaaggggat cgggtcagcg acattctgct
     1141 cagtaacgtg cgcttcgagc ttctgcccgg cggcaaaaac acattcaaca tccggggtgt
     1201 ggaacgcctg acgctggacc gcgtgacctt tatcgattcg tcggaatagg acaggaaatg
     1261 agggaaagcg tgatgcgggc gggacgattc agaagagggg ggtggacagc gcttctgatg
     1321 ggcgtccccg tatgggcttt ttgcgccgcc ggggatatcg tgcggccgat gggttcattt
     1381 gaccgcgcgg agtttacccg tcccgaagac gcggagcgcg tccgcagatc aggcaatttg
     1441 gcaggcacgt ccgaacccgg gaagaactgc tatatcaaag gctgggcaga gtatgacatc
     1501 gaggtgccgc aatccgactg gtatgaactc attgttccgg ggcaggcatg gggtgtcgag
     1561 taccggttcg acggcgatgt cgctgtctcg gcgggcagcg gcaagatcgg taatttctgg
     1621 cttgaggccg gacggcgcac ccttcgcgtc cagcgctatc actggacggg gtttcagcgt
     1681 atcgaatcct tcaccttgcg gccggcggag aagaagcccg ccaaacggct ccgtctatgg
     1741 atgccgggcg aaaaccggat cgtgcgcacg ggagaggtgc tgccgctccg gcttcaggcg
     1801 ggcgggccgg acggacagcc cggctgggtg acgatcctgc tgagcgaccc caagacgcag
     1861 gagataatct cgcaggcgga ggtcgccgtt ccggtaggcg aacgcccggc ggaacagccg
     1921 gtcacgctgg cctgtccgcg cgaggggctg ttcgaactcc gcgtgtccgc ggacgggagg
     1981 cggatcgaca accttgatgt gccggttcgg ccggtcgttg tggttgatac ccggccggcg
     2041 gcgcgcgggg gcgagctgcg gaaagcgctg attgcggaga tcgactgcgc ggtggccgcg
     2101 ccccagttct ccgggacggg cggtgcgaac gtggtccgga gcgaggccgg cgtctaccgc
     2161 gaatcgggcg atgcgggatt cttacgggcc cagcacaaca agcaggagtg cggctggttc
     2221 gcctatcggc tgggcgtgcc ggaggctcaa gcgccgtacg tgctggaggc cgactatccg
     2281 gacgacgcgt tccgcacgtt ctgcatcgcg attcgcgagg cggctccgga cgcatatccc
     2341 atcgcgggcg gcgtcgacag tggcgagtgc tacgcgctga cgcggcggat gcagacgcac
     2401 gcgctcctgt tctggccgcg cacccgcgat ctgcgggtct tgtttgtgac ggcgcacaac
     2461 gggcggcgcg gcgcggcggt gtcgaagatc cgcctctacc gcgtggaggg tgatctgccg
     2521 ttgcccagtg tgccgcctcg gggcgggcgt tcgttcggca actggtacga ggagggcagc
     2581 aacttcatgg ccgtttacgg cgcacccgac cggagcccgc gcggttttct gatcggggcc
     2641 gaccgctggg cgcggtccat cgcgtcggtg ggcggcgaca cgctcgtctc cacggtggcg
     2701 gtctaccaga tggcgatgta cccgtcacgt tacaacatcg acttcagcga gcctcactcg
     2761 ttcgacgtgg tgcgggtgtt gctgctgaaa tgcgagaaat acggcctcgg gttcatcggc
     2821 gagttccatc ccgagtgccg cgagttggat cggctggccg atccgacggg gcagatcacg
     2881 ccgaagccca acgggctcat ctcgaaagac ggcaagggcg gcaaagactg gagcgcaatc
     2941 ccgcgctttc atccgctcca tcccgttgac cgggagtggt acctcggcat gatcggcgag
     3001 ttcgtggacc gctacaagga ctcgcccgcc tttcgcggcg tgagcctgcg cctgatgggg
     3061 tgggccaacc ccgggctgaa caatttccac tcgctcgact ggggatacga cgacctaacg
     3121 gtcagcctgt tcgagaagga aacggggctt tcgacgggtg cgcgggcgga cgatccgaag
     3181 cgcttccgtg cccgctacga ctggctgatg gcgaacgccc gtggcgcgtg gaccgcctgg
     3241 cggtgccgca agatcatgga gcttcatgca gcgatccgtg accgcgtccg gcgcgcgcgt
     3301 ccggacctca ccgtgtacct caacaatttc ggattcgggg aagaggcggg aatcgccccc
     3361 aaacttctcc atgctctcga cggcgtggtg cttgtcaatg ccggacacgc ctatggccgc
     3421 cgggcctaca cctatgaggg tccgctggcc gattcgaaaa gacgcgataa actgcttgat
     3481 ccggcggcgc tgctcgcgct ccgcaacacg gaggggaaga cggccttcct gttcggcgcg
     3541 ggatacttcg aggccacgga gcgtgtcgtg ccgcccgcgt cgctcggctt tccggaggac
     3601 accaaacgga cttggatgag cggcgtcgtg aatcctgccg ggaggcatta tctggagcgt
     3661 tacgcgctgg cgttggccga agcggacgcc cagtaccttt cggatggcgg caacgcctac
     3721 acgctggggc agccgctgct gacggacttc ttgcgcgaat accgtgcgct gccggcgttg
     3781 ccgttccggc ctcgcgagga cgggcgtgac ccggtggcgg tgtgggaact gccggggcag
     3841 gagcggtttc tgttctatgc ggtgaaccgg gaacgcttcc ccgttcccgt cgcgatccgc
     3901 ttgaccggga caagcgaggt gcgtcggttc tcttccgcga agccgcagcg ccttgaggac
     3961 ggggtgctcc ggctgaccct gcaaccctat gagctggcgg ccttcgaggc cgggggcggt
     4021 gcggcgctcg cgggcgtgac cgaacaggtg ccgccggagg agttggcgcg tgcggagaaa
     4081 cagacggcgt ggatggaggc tctggcggtc gaggcgcggg ccgaccgtgt gggcggccag
     4141 ctttcggagg cgcaggccgc gcaggtcgtc gaagcggccg cagcatcccg tgccgcactg
     4201 gcctgcaggc acgtgtggca ggcgcggaca ctgcttgagc ggtcgggcct gattcaggtc
     4261 taccgcatcc tcaagcgctt cccgcccgcg ctctacgaca tgggggacga gtcggcgggc
     4321 gtccgttgac gtaccctaaa aagagcagtt gaactttcaa ctgccctttg gtttctggtt
     4381 ttcagggttt cagggtttca gggtttcatc caaatcgggg tcaggatcgc tatcgctatc
     4441 gggatcggga tcgctatcgg gatcgtcgac ccgcgacatt cgacccgcga cgatcgacag
     4501 ggacgcccgc gtttccgaca cc
//
LOCUS       DAOSRB010000220         4512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17428_length_4512_cov_7.551043, whole genome shotgun sequence.
ACCESSION   DAOSRB010000220 DAOSRB010000000
VERSION     DAOSRB010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17428_length_4512_cov_7.551043"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="PLZ74_09770"
     CDS             complement(<1..1571)
                     /locus_tag="PLZ74_09770"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020621142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOR98649.1"
                     /translation="MSKTIWCENGFPKVGIRPIIDGRRRGVRESLETQTMNMAKSAAK
                     LISESLTYPDGSPVQCVIADTTIGGCAEAAACANKFERENVGVSLSVTPCWCYGTETM
                     DTAPHTPKAVWGFNGTERPGAVYLAAMLAGYAQRGFPAYGIYGREVQDRTDTTIPDDV
                     KEKLLRFVKAGLAVAMMRGKSYLAVGTSSMGIAGSFIDPDLFQDYLGIRVENVDMCEL
                     DRRAVEGIYDKAEYKKALAWAKKFCKEGKDINKKRLQKSRAAKDADWEYVIKMTLALR
                     DLMVGNPVLAEMGLGEEAVGRNAIAAGFQGQRQWTDYKPNGDFSEAILNSSFDWNGIR
                     APYIVATENDALNGICMLLMYLLTNRAQIFADVRTFWSADAARRVTGKRMTGLNANGF
                     IHLLNSGAACLDATGKQRFKGKPTMKPFWEITAKEAEACLKATRWMPANVEYFRGGGF
                     SSSFLSEGGMPMTMTRISRVKGLGPILQIAEGWSTTLPAGVTETLMARTDPTWPCTWF
                     VPRLTGKGAFRDVYSV"
     gene            1829..4459
                     /locus_tag="PLZ74_09775"
     CDS             1829..4459
                     /locus_tag="PLZ74_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOR98650.1"
                     /translation="MKPNCPDLFSFRTAMASLLLAATLSAVARAQAPSPALLLIPQPQ
                     SLRLEASGGAFTLNARTPLSGEAALVEVVRGVLSEHAALDALPSGPGGITVSKPSDPA
                     DALWKNDQAYLLRVLPAGVTIEAAGRDGARYAAQTLVQLLRIAPSVPALTIRDWPAIR
                     TRLAMIATDQGGFQVIDPEYWKRLIRELASYKINAIMPYFDGGTYKYRKYPFLGNKGD
                     DGFTLEKARMLSEYAEQRGVRLIPQQNSLGHLGGVLGHRELQHLRDGGGTINMVSPDS
                     LAFLGDLYDELTQAFPYAHAIHVGGDEFGHDFGKNPQVAARIAEIGKAGVYGAFMTAL
                     RDKLRSRNREMMIWKNEQGLTLEAADRMPRDIAVFDWNYDARQDYPSLDTLLEAGFTQ
                     PWATPAVTRYYSRGNDWDATFANIRNFAAAGIRRRVPGLCTCTWVHGMWGGRNLFELN
                     LYGLVFSAACGWNPPPENETTPFTRAFALQWLGCRDPQAAVWVRDGIHTPYGTRTEQK
                     FWRDNRVQEKYACSPFSTLMESVATTPALEQDARELIAFCDRAETALDTLARSATRNL
                     RTLDYFKHDVRIQRLAANRILTAAELSRWVAGLKIPKPVPERNLLRLTFDSLPTNDAV
                     RVSPTARISGGTLATVPADHWARKGLDIGPLPLPESGVQIEYDVCPLQSGNQYQQFAS
                     TRPSTHHFMAFLGKNRRFSLHTHAGGVWSEQATLTEPCATGTWYRCTVQIRRDNLSFR
                     AVERETGKPVCRSGFVPIDPVGPQLHFSLTDNHGATGNRAPASLWDQVTVSTLKPVPD
                     QPTTPPADLLVRFKRLIQDHNTIEETFRKSVLEAGGGSADTGNLSKGATQFRSRQGRE
                     DTEKLLRMLTAGRLPVNLCE"
BASE COUNT          839 a         1520 c         1327 g          826 t
ORIGIN      
        1 acgctgtaga cgtcgcggaa agcgcccttg cccgtcaggc gcggcacgaa ccacgtgcag
       61 ggccaggtcg gatccgtgcg cgccatcagc gtctcggtga cgcctgcggg cagcgtggtg
      121 gaccatccct cggcaatctg cagtatcggg ccgagcccct tgacacggct gatgcgcgtc
      181 atggtcatcg gcatgccgcc ctcggagagg aagctggagg agaagccgcc gccgcggaag
      241 tactcgacgt tggccggcat ccagcgcgtc gccttgagac acgcctccgc ctctttcgcg
      301 gtgatctccc agaacggctt catcgtgggc ttgcccttga aacgttgctt gccggtggca
      361 tcgagacagg ccgcaccgga gttcagcagg tggatgaacc cgttggcatt gaggccggtc
      421 atacgcttgc cggttacacg cctagccgcg tccgcgctcc agaaggtgcg cacatcggcg
      481 aagatctggg cacggttggt cagcagatac atcagcagca tgcagatgcc gttgagcgcg
      541 tcgttttccg tcgccacaat gtacggcgca cggatgccgt tccagtcgaa cgagctgttg
      601 aggatcgctt cggagaagtc gccgttgggt ttgtagtcgg tccactggcg ctgcccctga
      661 aagcctgccg caatggcatt acgccccacc gcctcttcgc cgaggcccat ctccgccaac
      721 accggattgc cgaccatcag atcgcgcagg gccagcgtca ttttgatgac atactcccag
      781 tcggcatcct tggcggcgcg cgatttctgg agccgcttct tattgatgtc cttgccttct
      841 ttgcagaact tcttggccca cgccagcgcc ttcttgtact cggccttgtc gtagatgccc
      901 tccacggcgc gccggtccaa ttcgcacatg tcaacgttct caacgcgaat gccgaggtag
      961 tcctgaaaga gatccggatc aataaacgaa cccgcgatcc ccatcgacga ggtgcccacg
     1021 gcaagatagg atttgccgcg catcatcgcc actgccagcc cggccttgac gaagcgcaac
     1081 agtttttcct tcacgtcgtc cggtatcgtc gtgtcggtgc gatcctgcac ctcacgcccg
     1141 tagatcccgt aagccgggaa gccgcgttgc gcataacccg cgagcatcgc ggccagatag
     1201 accgcgccgg gacgttcagt gccgttgaag ccccagaccg ccttaggcgt atggggtgcc
     1261 gtgtccatcg tctcggtgcc gtagcaccag cagggcgtga cgctcaacga gacccccaca
     1321 ttctcgcgct cgaacttgtt cgcgcaagcg gcggcctcgg cgcagccgcc gatcgtggta
     1381 tccgcgatca cacactgcac cggcgagccg tcgggatagg tcagactctc ggaaatcagc
     1441 ttggccgcgc tcttggccat gttcatggtt tgcgtttcta acgactcacg caccccgcga
     1501 cgcctgccgt caatgatcgg gcggatgccc actttcggaa aaccgttttc gcaccaaatc
     1561 gttttactca ttcgtgaacc ctccttatga ataaggaata gtgtaccacc cgatcctcct
     1621 tttgactagt ccgctttgcg caatttaaaa ttttacaagg gaaccggtta tctgaaagag
     1681 ggacgggcga gccggaaacg cgcgcacccc ggccgccaac cgattgccgg ttcaagcgtt
     1741 cgccgggttg ccgggaaccg ttttttttgg cagactcggg gtatgggcga accggttctg
     1801 gttccgcccg tcctgaaaaa ggagacacat gaaaccgaac tgtcccgatc ttttttcgtt
     1861 ccgtaccgca atggcatcgc tgctgctggc cgcaaccctg tccgccgtgg cgcgcgcgca
     1921 ggctccctcc cctgccctct tattgatccc gcaaccccaa tcgctgcgac tcgaggcctc
     1981 cggcggggct ttcaccctga atgcccgcac accgctctcc ggcgaggccg ctctggttga
     2041 ggtggtgcgc ggagtgctgt cggaacatgc cgcccttgat gcgctgccgt ccggtcccgg
     2101 cggcatcacg gtgagcaagc cctccgaccc ggctgacgcg ctctggaaaa atgaccaggc
     2161 ctatctgttg cgcgtactcc ccgcaggcgt gaccattgag gccgcgggcc gcgacggtgc
     2221 ccggtatgcc gcgcaaacac tggtccagct gctccgcatc gcgccgtccg tgcctgcgct
     2281 cacgatccgc gactggcccg cgatccgcac acgtctggcg atgatcgcga ccgaccaagg
     2341 aggattccag gtgatcgacc ccgaatactg gaagcgcctg atccgcgagc tggcctctta
     2401 caagatcaac gcgatcatgc cctattttga tggcggcacg tacaagtacc gcaaataccc
     2461 gtttctcggc aacaagggcg atgacgggtt cacgctcgaa aaggcccgga tgctgagcga
     2521 gtatgcggaa cagcgcggtg tgcggctcat ccctcagcag aactcgctgg gccacctcgg
     2581 cggcgtgctg ggccacaggg agttgcagca tctgcgcgac ggaggcggca cgatcaacat
     2641 ggtcagcccc gattcgctcg cctttctcgg cgacctctat gacgaactga cgcaagcctt
     2701 cccgtacgcg cacgcgatcc acgtgggggg cgatgagttc ggccacgatt tcggcaagaa
     2761 cccgcaggtg gcggcccgta tcgcggagat cggcaaagcc ggggtgtacg gcgcgttcat
     2821 gaccgccctc cgcgacaagc tccgcagccg caaccgcgag atgatgatct ggaagaacga
     2881 acaggggctc accctcgaag ccgcagaccg gatgccgcgc gacatcgcgg tgttcgactg
     2941 gaactacgac gcccgacagg attatccctc gctggacacg ttgttggagg cgggcttcac
     3001 ccaaccttgg gccaccccgg ccgtgacccg ttattacagc agagggaacg actgggacgc
     3061 aacgttcgcg aacatccgca acttcgcggc ggccggtatc cgtcggcgtg tgcccggact
     3121 ctgcacctgc acctgggtcc acggcatgtg gggcggacgc aacctgttcg aactgaacct
     3181 ctacggactg gtcttcagtg ccgcttgcgg ctggaatccg cctcccgaaa acgagaccac
     3241 ccccttcacc cgcgctttcg cgcttcaatg gctgggatgc cgcgatccgc aagccgccgt
     3301 ctgggtccgc gacggcatcc acacgccata cggcacacgc acggagcaga aattctggcg
     3361 cgataaccgg gtgcaggaaa aatacgcctg ctctcccttc tccacgctga tggagtcggt
     3421 cgccacaacg cccgcgctgg agcaagacgc gcgtgaactg atcgcgttct gcgatcgtgc
     3481 ggaaacggcg ctcgacacgc tcgcgcgttc cgccacccgg aacctccgca cactcgatta
     3541 cttcaaacac gatgtccgga tccaacgtct ggccgcgaac cgcatcctga ctgccgcgga
     3601 actgtcgcgc tgggtcgcgg gcctgaagat tcccaagccg gttccggagc ggaatctgtt
     3661 gcgtctcacg ttcgactccc ttccaacgaa cgacgccgtg cgggtcagcc cgacagcccg
     3721 catctccggc ggcacgctcg ccaccgtgcc cgcggatcat tgggcgcgga agggactcga
     3781 tatcggtccg ctgccattgc ccgaaagcgg cgtgcagatc gaatatgacg tgtgcccgct
     3841 gcaatccggg aaccagtatc agcagttcgc ctcaacccgt ccctcgacgc accacttcat
     3901 ggccttcctc ggaaaaaacc ggcgtttcag cctgcacacc cacgccggag gcgtctggag
     3961 cgaacaggcc acgctcaccg aaccatgcgc gaccggaacg tggtatcgct gcaccgtgca
     4021 gatccgacgc gacaacctct ccttccgggc cgtggagcgc gagaccggca aaccggtctg
     4081 ccgctcgggt ttcgtgccga tcgatcctgt ggggccgcag ctccacttct ccctcacgga
     4141 caatcacggc gccacgggaa accgcgcgcc cgcctcgctg tgggatcagg tgaccgtctc
     4201 cacgctgaag cccgttccgg accagccgac caccccaccc gccgaccttc tggtccgctt
     4261 caagcggctt atccaggatc acaacacgat cgaggagact ttccgcaaat ccgtgctgga
     4321 ggcaggcggc ggttcggcgg acaccggcaa cctcagcaaa ggagccacgc agttccgcag
     4381 ccgccaaggc cgcgaagata cggagaaact gctccgcatg ctcactgcgg gccgtctgcc
     4441 ggtgaacctg tgcgagtgag tggccctttt tgaacgttca atctaaaaag agccgttgaa
     4501 cgggtagaat tc
//
LOCUS       DAOSRB010000221         4503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17480_length_4503_cov_6.608363, whole genome shotgun sequence.
ACCESSION   DAOSRB010000221 DAOSRB010000000
VERSION     DAOSRB010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17480_length_4503_cov_6.608363"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLZ74_09780"
     CDS             <1..572
                     /locus_tag="PLZ74_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98651.1"
                     /translation="KDWSEKIVDMHSESENQYGPFRYGPAYPFNAMEPDLEASELPFP
                     PMYLTLNYRYSAAKNESLTLQIELLDPMVESFMSGGNTFLALAAKETDATRRDNARRL
                     GWHGQYMARCCLTARNVKAAVIAERAKDRAKVVEIARAEYANAKAALKLLHADSRLGW
                     EPTMLYRGGPEMIEWKLRYMEEHYGIGDQ"
     gene            627..1151
                     /locus_tag="PLZ74_09785"
     CDS             627..1151
                     /locus_tag="PLZ74_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOR98652.1"
                     /translation="MKTQKLEVVEVSKLVKTPGDSIMKRRTVLSAFLLLAAVSTALGF
                     APRTYDVTSPSMGKAVPVTIVLPKGYEDDPARRHPVVYLLHGANDTDFHAYHPLFRKA
                     ADENGVILVAPCGTLSWWMDSPVDPKHRYETFVTKELIPWVDARYRTIPDRMHRGLAG
                     NSMGGLFWYVWRAA"
     gene            complement(1156..1404)
                     /locus_tag="PLZ74_09790"
     CDS             complement(1156..1404)
                     /locus_tag="PLZ74_09790"
                     /inference="COORDINATES: protein motif:HMM:NF039850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5763 domain-containing protein"
                     /protein_id="HOR98653.1"
                     /translation="MKPTMKRSVITLAAALIAAAALAASVQCRGTTKRGARCKNKTTD
                     ASGYCHLHKSQAYRHASAAATNGTRRAHGVAATNSLPR"
     gene            complement(1466..2575)
                     /locus_tag="PLZ74_09795"
     CDS             complement(1466..2575)
                     /locus_tag="PLZ74_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98654.1"
                     /translation="MKMANQTRGPFAASPLMPRSLWDKLLFRENPGNAYVELVNALAR
                     TDDIRSIPEDFADTLNRKYRTNLHSRRRADLDRIYADFLRHCLADLAFTAREAEEANR
                     LRRLFLITDAKHAKIFSSVADETHRRAVKRVIADGKVTDNERRALEYLAASLLISERT
                     SKAIYKDEVDKMLQGRLDAAVSDCMLSPQEEAELQELAAGLGVELTHSAATARTLDSY
                     RAMWRVRYGDLPTLDSGINLRRGEVCYMKRAVEWHEMRRQRVGATYSGPTMRLKIAKG
                     VYWRTGTLGIKPVTRDTLVKIDSGHAYITNKRLLFTGAMKNTSIPLNRILDITKYSDG
                     VGIEKDAGKSPVLVFHEGIDIFCAVLSRVISEQAP"
     gene            complement(2572..2829)
                     /locus_tag="PLZ74_09800"
     CDS             complement(2572..2829)
                     /locus_tag="PLZ74_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR98655.1"
                     /translation="MREKRGNKPQSQMAKELGMKQPMHARYESGMTLPSAELLFHICS
                     KLGVSADWLLGLSDDESGRQPATVAGGGSRVNEKAKERKEA"
     gene            3111..4202
                     /locus_tag="PLZ74_09805"
     CDS             3111..4202
                     /locus_tag="PLZ74_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98656.1"
                     /translation="MTNAMNATTLFGDGSIRIGGFSKFIINGQAYRCGLNPSTADGLL
                     DFVVYDNGQFDASLEDATYLNNSALAGSVTRITASDSATVKFGHNAVVGYAAGSTGEL
                     ILKDSVTCQNGLYGFTFGRGGSATDISTGILRMSGGTFTIGSSKTTTYPDLYGFVFGN
                     GAATNATGEIWNKGFGYISGGTITVCQGIHIGDGNAYGFVEQSGGKIQMSNNNSYPVN
                     VGNHHGTGVYRMTGGTFHTPNSTVSVGANNGNGMLEFGAGTGTCTAKNMNLAGVGATL
                     KFVLGADGSVIKPNVTDTLTIDPGAKIVVDTTACAEPKSVDLMTFGAKSGSFADADIE
                     IIGIKGALVSQSDAKLRLQINKGTVVIIR"
BASE COUNT          892 a         1273 c         1388 g          950 t
ORIGIN      
        1 ggaaagactg gtcggagaag atcgtggaca tgcactccga gagcgagaac cagtatgggc
       61 cgttccgcta cggcccggcc taccccttca atgcgatgga acccgacttg gaggcttcgg
      121 aactgccgtt cccgccgatg tatctgaccc tgaattatcg gtatagcgcg gcaaagaacg
      181 agtcgctcac gctgcagatc gaactgctgg atcctatggt ggaatccttc atgagtggcg
      241 ggaacacgtt tcttgcgttg gcggcgaagg agacggacgc gacccgacgg gacaacgccc
      301 gccgcctcgg ctggcacggc caatacatgg cgcgttgctg cctcacggca cgcaacgtca
      361 aggccgccgt gatcgccgaa cgcgcgaaag accgcgcgaa ggtggtagag atcgcccgtg
      421 ccgaatacgc gaatgcaaag gctgcgctca agcttctaca tgctgattca cgcctcggct
      481 gggagcccac gatgctctac cgcggcggcc ctgaaatgat cgaatggaaa ctccgctaca
      541 tggaagagca ctacggtatc ggagatcagt aaacctaaaa agagcagttg aactttcgtg
      601 gaaagcgatg aaaagaaagt gacggcatga agacccaaaa actggaagtc gtggaggtgt
      661 ccaaactcgt gaagacgccc ggagattcga tcatgaagcg ccggacggtt ctgtcggcct
      721 ttttgttgct ggcggcggta tcaacggccc ttggttttgc tccgcggacc tacgatgtca
      781 cgagcccctc gatgggcaag gccgttcccg tgacaatcgt cctgccgaag ggctatgagg
      841 acgatccggc gcggcgtcat ccggtcgtct acctccttca cggcgcgaac gacaccgatt
      901 tccacgcata ccatccgcta ttccgcaagg cggcggatga aaacggcgtt atccttgtcg
      961 cgccgtgcgg cacgctgagc tggtggatgg acagtcccgt agaccccaag caccggtacg
     1021 aaacatttgt gaccaaggag ttgattccgt gggtcgatgc gcgttaccgc acgattcccg
     1081 accgcatgca ccggggcttg gccggcaact ccatgggcgg ccttttttgg tatgtctggc
     1141 gggcggcctg acctgtcagc gcggcagcga gtttgtcgcg gcaaccccgt gggcgcggcg
     1201 ggtgccgttc gtcgcggcgg cggacgcgtg cctgtaggcc tggcttttgt gcaggtggca
     1261 gtagccggag gcgtcggtgg tcttgttctt gcaccgcgcg cccctcttcg ttgtccccct
     1321 gcactgcacg gaggcggcga gcgccgccgc cgcgatcagc gcggcggcca gcgtgatgac
     1381 gcttcgcttc attgtcggct tcatgcttct ttcctttcct tccttttgtg atcacacgcc
     1441 ccgcatgcgc gcggctgttt ttgtttcacg gcgcctgctc ggagatcacg cgggagagca
     1501 ccgcgcagaa gatgtctatg ccttcatgga agacgaggac ggggctcttg ccggcgtctt
     1561 tctctatgcc cacgccgtcg ctgtatttgg tgatgtcgag aatccggttc agggggatgc
     1621 tggtgttttt catcgcccct gtgaagagca ggcgcttgtt cgtgatgtag gcatggccgg
     1681 agtcgatctt caccagggtg tcgcgcgtga ccggcttgat ccccagcgtg ccggtccgcc
     1741 agtacacccc ttttgcgatc ttgaggcgca ttgtcgggcc ggagtaggtc gcgccgaccc
     1801 gctgccggcg catttcgtgc cactccacgg cgcgcttcat gtagcagacc tcgccccgcc
     1861 gcaggttgat gccggaatcg agcgtgggga gatctccgta acgcacgcgc cacatcgccc
     1921 tgtaggaatc aagggtgcgc gccgtggcgg cgctgtgcgt aagctcgacg ccgaggccgg
     1981 cggccagctc ctgtagctcc gcctcttcct gcggcgacag catgcagtct gacacggccg
     2041 cgtcgagccg cccctgaagc attttatcca cttcgtcttt gtagatcgct ttactggtac
     2101 gctccgatat gagtaacgac gcggccaaat attcgagtgc cctgcgctca ttatctgtga
     2161 ccttgccgtc cgcaataacc cgttttaccg cgcgacgatg cgtttcatct gcgacagagc
     2221 taaaaatctt ggcgtgcttt gcgtctgtaa tcaggaagag gcggcgcagg cggttggcct
     2281 cttccgcctc tctggccgtg aaggcgaggt cggccaggca gtggcggagg aaatctgcat
     2341 agatccggtc taggtctgcg cggcggcgtg agtgcaggtt cgttctgtat ttgcggttga
     2401 gggtgtctgc aaagtcttct gggatggagc ggatgtcgtc tgtccgggcg agggcgttca
     2461 ccagctcgac gtaggcgttc ccggggtttt cacggaagag gagcttgtcc cagagggagc
     2521 ggggcataag cggggacgcg gcgaacggcc cgcgcgtttg gtttgccatc ttcatgcctc
     2581 tttcctttcc ttagcctttt cgttcaccct ggagccgcca ccagcgacgg tcgcgggttg
     2641 gcggcccgat tcgtcatctg acagccccaa aagccaatcg gcggacacac cgagttttga
     2701 gcagatgtga aacagcaact cggcactcgg tagcgtcatt ccgctttcat atcgtgcatg
     2761 cattggctgt ttcattccaa gctctttcgc catttgcgat tgcggtttgt tcccccgttt
     2821 ttcacgcaaa cgggcagaga aaacattttt gtcaaaaaaa ctcgcataat aacacttagt
     2881 tattggcagg ttaataactg actagttatt atccccgctg taacggcaac gtgccacgta
     2941 ggaaaggtag caaatgaaga tgagcgggaa aaggaaatcg tcaatccggc gtgagacgcg
     3001 cagcgtgatt ccggggatat gccgggacgc gcggcggcac ggcgtctctc ggatgcacct
     3061 gtgggcggtt cttacggggc ggcgcgatcg atctttccgg tgacgctgtc atgaccaacg
     3121 ccatgaatgc cactacgctt ttcggcgacg gttcgatccg catcggcggt ttctcgaagt
     3181 tcatcatcaa cggccaggcc tatcgttgcg ggttgaatcc gagcacagcg gacgggcttc
     3241 tggattttgt cgtctacgac aatggacagt ttgatgcgtc actagaggat gcgacctatc
     3301 tcaacaactc ggctctcgcc gggagcgtga cgcggattac ggcatccgac tcggcgacgg
     3361 tgaaattcgg ccataacgca gtcgtcggtt acgccgccgg atcgacgggc gagttgattc
     3421 tgaaggactc ggtgacctgt cagaatgggc tgtatggttt cacattcgga agaggcggtt
     3481 ccgcgactga tatttcgacc ggcatcctca ggatgtccgg cggtacgttc acaatagggt
     3541 cgtcaaaaac cacaacttat ccagacttat atgggtttgt cttcggtaac ggcgcggcga
     3601 ccaatgcgac gggagagatc tggaataagg gcttcggtta tatctccggc ggcacgatca
     3661 cggtttgcca gggcatccat atcggcgacg gcaatgccta cggatttgtt gagcagtcgg
     3721 gcggcaaaat tcagatgtcg aacaacaaca gttaccccgt caatgtcggc aatcatcacg
     3781 gtaccggcgt gtatcgcatg accggcggca cgttccacac cccaaattcc acggtcagcg
     3841 tcggcgcgaa caacggcaac ggcatgttgg agttcggcgc aggcacaggc acctgcacgg
     3901 caaagaacat gaatctcgcg ggcgtggggg cgaccctgaa gttcgtgctc ggcgcggacg
     3961 gttcggtcat caagccaaac gtgacggaca cgctgaccat cgatccgggc gcgaagatcg
     4021 tcgtcgacac aacggcgtgt gcggaaccga agtcagtcga tctcatgacc ttcggggcaa
     4081 agtccggttc gttcgcggac gcggatatcg aaatcatcgg gatcaagggt gcgcttgttt
     4141 cgcagagtga tgcaaagttg cgccttcaga tcaacaaagg cactgtcgtc ataatccggt
     4201 aagatgccgg aagaggcgca tgtcctgcgc cgtcgcggag caacagaaga aaccgtcgtc
     4261 ccctccgctg tgcgtagaag tgccggcaca ttcaccggcg cgggcggttt cacgcccacc
     4321 gcacccgttt tacaaacagc ggaacagacc agagttgaaa gcttgaacct aaaaggagca
     4381 gttgaacggg tagaattcta gcaaagcccc gcaaacattg ggttttgacg tattgagatg
     4441 cgggcggcgc ccgcaatctc aaaggctgta agactgtgag gctgtaagac tgtgaggcgg
     4501 ttg
//
LOCUS       DAOSRB010000222         4477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17597_length_4477_cov_10.049525, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000222 DAOSRB010000000
VERSION     DAOSRB010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17597_length_4477_cov_10.049525"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /gene="carB"
                     /locus_tag="PLZ74_09810"
     CDS             <1..1419
                     /gene="carB"
                     /locus_tag="PLZ74_09810"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOR98657.1"
                     /translation="ETVSTDYDTSDKLYFEPVTLEDVLQIYENEKPEGVIVQFGGQTP
                     LNIARELADAGVKILGTSIDSIDTAEDRDLFRKMMERLAIPMPESGMAANIDEAIRVA
                     GSIGYPIMIRPSFVLGGRAMEVIYDEEMLREYVAKAVGVTPDRPLLLDRFLKEALECE
                     ADALSDGREVYIPAVMEHIELAGVHSGDSACVLPPVSIPDRHLDTILAYTRKIAQELQ
                     VVGLMNMQYAIENDTVYVIEANPRASRTVPLVSKVCDTQMARIATRLMLGASIADLDL
                     RPKVIPHYGAKEAVFPFDKFPEVDPVLGPEMRSTGEVLGMASTFGLAYYKSQEAANAR
                     LPTEEGTLLVSLSEKPDSAAKVISAFVKLGFRIIGTEGTILWLKEHGVPGEVVQKINE
                     GRPNVLDAIVNRQVCLVINTPSARRDARADDASIRKAAIKYKVPYLTTLAAAAAAAQG
                     IRSARDSAGGVKSIQAYHANIR"
     gene            1875..2558
                     /gene="cmk"
                     /locus_tag="PLZ74_09815"
     CDS             1875..2558
                     /gene="cmk"
                     /locus_tag="PLZ74_09815"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOR98658.1"
                     /translation="MSRVIAIDGPSGAGKSSVSRRVGQHLGFLHVDSGALYRIMTWQC
                     LLNGIDTSDPEAVAAFAQTVEVECEPRDGAIVYRVQGQEPGDAIRTPEINRHASPVAT
                     VKAVRDRVTGWLRGMTRFGDLIVEGRDITTAVFPDSPARFYLVASAEARAERRRKEEV
                     EKGIANQSAEEVKRSLLARDAIDSTRQHAPLRKADGALEIDSTYLTLDQVVQMVLDAL
                     PTAWKGGDA"
     gene            2714..3982
                     /locus_tag="PLZ74_09820"
     CDS             2714..3982
                     /locus_tag="PLZ74_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOR98659.1"
                     /translation="MRKDTHRKFALNFLLFAILTAVSAFGWGDAHSVITRAALSTLPS
                     SDAALLGNEREPLSAGYCLIPDWVYKKDGNERFVWDFMGTESYSGRFENFHLPWNAAS
                     NYEMLEYYIRHAVASFKTNDAASAARHLGVLAHMLEDWTCPAHSSVGDNMYRLLGQYM
                     PAPAGKEHMLLHAHLESGGFDLSGYTHTPVALGATTEEVAFRLLSRAQAGTVFARGRV
                     LDIVRAVYAEDKQASAAAQRPSAILGADLVADAFRSVCALARGEAPPPAPIALTPYWP
                     NEAPSLFYPQAAFFSSPYWGHPQRDMILRDGKDPVPLALQISGERTEVKGLGIGVKRI
                     SWFLPGNLFSRIMLRVGLHADLGTDGQVTFRVFGNDAELHKTELRGTAQSVPIDLPIT
                     GVTNLAFLASPINRSKANYVVLADPMLIPK"
     gene            complement(3997..>4477)
                     /locus_tag="PLZ74_09825"
     CDS             complement(3997..>4477)
                     /locus_tag="PLZ74_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013464554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HOR98660.1"
                     /translation="IMVQWDETSPRPYSRHNLIQGTRGTFAGFPNRIALDYRKEDLPE
                     PVRKALGDKPRTNYHGWDQNMAPWFEAYDHPLWKRMGAEAKRVGGHGGMDFIMNWRVI
                     YCLRNGEPLDQTVYDAAAWSAIGPLSERSVTERSRSVDIPDFTRGVWERTQPFGIVS"
BASE COUNT          865 a         1478 c         1280 g          854 t
ORIGIN      
        1 gagacggttt ccacggacta cgacacctcg gacaaactgt acttcgagcc cgtcacgctc
       61 gaggatgtgc tccaaatcta cgagaacgaa aaaccggaag gcgtaatcgt gcagttcggt
      121 ggccagaccc cgctcaacat cgcccgcgaa ctcgcggatg ccggtgtgaa gatcctcggc
      181 accagcatcg actccatcga caccgctgaa gaccgcgacc tcttccgcaa aatgatggag
      241 cggctcgcga tccccatgcc tgaatccggc atggccgcca atatcgatga ggccatccgg
      301 gtcgccgggt ccatcggcta tcccatcatg atccgtccct cgttcgtgct cggcggacgc
      361 gcgatggagg tgatctatga cgaggagatg ttgcgcgagt acgtggccaa ggcggtgggc
      421 gtcaccccgg accggcccct gttgctcgac cgcttcctga aagaggcgct cgaatgcgag
      481 gccgacgccc tctccgacgg tcgggaggtt tatattcccg ctgtcatgga acatatcgaa
      541 ctcgcaggcg ttcactcggg agactcggcc tgcgtgttgc cgccggtctc gattccggac
      601 cgccatctcg acaccatcct cgcgtacacc cgcaaaatcg cccaggagct gcaggtggtg
      661 ggactcatga acatgcagta cgccatcgag aacgacacgg tgtatgtcat cgaggccaac
      721 ccgcgcgcct cgcgcaccgt gccgctggtc tccaaggtct gtgacaccca gatggcccgc
      781 atcgctacac gcttgatgtt gggcgcctcc atcgccgatc tggatttacg acccaaggtc
      841 atcccgcact acggtgccaa ggaggcggtt ttcccctttg acaaatttcc ggaggttgac
      901 ccggtcctcg gccccgagat gcgttcgacc ggtgaagtcc tcggcatggc ttcgactttc
      961 ggcctcgcct attacaaatc gcaggaagcc gccaacgcgc ggctgccgac ggaagagggc
     1021 acactgctcg tgagcctctc cgaaaagccc gactccgcgg cgaaagtcat cagcgctttc
     1081 gtcaaactcg gcttccgcat catcggcacc gagggcacca tcctctggct caaggaacac
     1141 ggcgtacccg gcgaagtggt ccaaaaaatc aacgaaggcc gtcccaacgt tttggatgcc
     1201 atcgtcaacc gccaggtctg tctcgtgatc aacacccctt ccgcgcgtcg tgacgcgcgt
     1261 gccgatgatg cctcaatccg taaggcagcc atcaaataca aggttcccta cctcaccacc
     1321 ctcgcggctg cggcggccgc ggcgcagggc atccgttcgg cgcgtgacag cgcgggcggc
     1381 gtgaaatcca ttcaggcgta ccacgccaac attcggtaac ccggaggccg tctcctccgg
     1441 tttctggttt cgcttcggcg tcgggcgttc gttcgccttg acgcccccgc gtccttttta
     1501 taaggatgta aggaaaaaag gagttgttgt gacgctcgcc aaactcacca gtaaggatca
     1561 attgaccctt cggcctctca gcgccgtgca tgaaaaaatg gagcagctca acataaccga
     1621 ggcggatatc gggaaggctg tggcgtggtc gcaataagag aggttgactc gcgacccgcg
     1681 acttgtgact tgcggcactg cccaaaaaac tttcaccgct caacacccaa cgttcaacgc
     1741 cgaagtgaaa aaactcctaa ctcctaaatc ctaaatccta aatcctaaat cttaaatccg
     1801 catggtgcgt accatgcgct tgaaccgcac cgttctgagt ggtaagatca ggccatcaat
     1861 aaaggaaccc cgatatgagt cgagtcatcg cgattgacgg cccctccggc gcaggcaagt
     1921 cgtctgtgtc caggcgcgtc ggccaacacc ttggtttttt gcatgtcgat tccggcgcgc
     1981 tctaccgcat catgacctgg cagtgcctgc tgaacggcat tgacacgtcc gatccggagg
     2041 cggttgccgc cttcgcgcag acggtcgaag tcgagtgcga accgcgtgac ggcgcgatcg
     2101 tctaccgcgt gcagggccaa gaaccgggcg atgccatccg cacaccggag atcaaccgcc
     2161 acgccagccc agtcgccacg gtcaaggctg tgcgcgaccg cgtgaccggc tggctgcgcg
     2221 gcatgacgcg cttcggtgac ctgatcgtgg aggggcgcga catcacgacc gccgtgtttc
     2281 cggactcgcc ggcgcgcttc tatctcgtgg ccagcgccga ggcgcgcgcc gaacgccgcc
     2341 gcaaggagga ggtcgagaaa ggcatcgcca accagtcggc ggaggaggtc aagcgctcgc
     2401 tgttggcgcg cgacgcgatc gactccacgc gccaacacgc cccgctgcgc aaagcggacg
     2461 gcgcgttgga aatcgacagc acttacttga cgctcgacca agtggtgcag atggtgctcg
     2521 acgcactgcc aactgcatgg aaggggggtg acgcatgacc ctcgctggct ggattacgat
     2581 ggtactctgc tggacggtca tcaccggttt ctgtcttttc cttgtcttca aaacattgca
     2641 gtccgatccg ccctcctgat cgtgccgcaa cgtgcccccc cccccgattc ccatctcatc
     2701 gaggacataa ggaatgcgta aagacacgca cagaaaattc gcgctgaact ttcttctgtt
     2761 cgcgatcctg accgccgtat ccgctttcgg atggggcgac gcgcacagcg tcatcacccg
     2821 cgccgcactg tcgacgcttc cgtccagcga cgcggccctg ctcggcaatg agcgtgagcc
     2881 gctcagtgcc ggatattgcc tgattccgga ttgggtctac aaaaaggacg gcaacgagcg
     2941 ctttgtttgg gatttcatgg gaacggagtc ctattccgga cgctttgaga acttccacct
     3001 cccgtggaat gccgcatcca actatgaaat gctcgaatac tacatccgcc atgcggtcgc
     3061 gtcctttaaa acaaacgatg cggcctctgc ggctcgtcac ctcggtgtgc tcgcgcatat
     3121 gctggaagat tggacttgtc ccgcgcactc atccgtgggc gacaacatgt accggcttct
     3181 cggccagtac atgcccgccc cagccgggaa agagcacatg cttctccacg cgcaccttga
     3241 aagcggcggc tttgatctgt cgggctacac gcacacgcct gtcgcgctgg gtgccaccac
     3301 agaggaggtg gcgttccgcc ttctgtcacg cgctcaggct ggcacggtct tcgcgcgcgg
     3361 acgcgtgctc gacatcgtcc gagcggttta tgcagaagac aagcaggcct ccgccgcggc
     3421 ccagcgcccc tccgctattc tgggggctga cctcgtcgcc gacgccttcc gctcggtctg
     3481 cgcgcttgcc cgcggggagg cgcccccgcc cgcgccgatc gcgctgaccc cgtactggcc
     3541 gaacgaggcc ccgtcgcttt tttacccgca ggccgcgttt ttcagctcac cgtactgggg
     3601 ccatccgcaa cgcgacatga tcctccggga cggaaaagat cccgtacccc tcgcgctcca
     3661 aatcagcggt gaacgcaccg aagtgaaggg gttggggatc ggcgtcaaac gtatcagttg
     3721 gttcctgccc ggcaacctgt tttcgcgaat catgctccgc gtgggtttgc acgccgacct
     3781 cggcaccgac ggccaggtca ccttccgggt tttcggcaac gatgccgaac tgcacaagac
     3841 ggagttgcgc ggcacggcgc agagcgtacc gatcgacctt ccgatcaccg gtgtcacgaa
     3901 cctggccttt ctcgcctctc cgatcaaccg ctccaaggcc aactacgtgg tgctcgccga
     3961 ccccatgctg attccgaaat agggtcggtt cgtcgtttac gacacgatgc cgaagggctg
     4021 tgtgcgttcc cacaccccgc gggtgaagtc cgggatatcc accgaccggc tacgctcagt
     4081 cacggagcgt tcgctcaacg gaccgatcgc gctccacgcc gcggcgtcgt agaccgtctg
     4141 gtccagcggc tcaccgttgc gcaggcagta aatcacgcgc cagttcatga tgaagtccat
     4201 gccgccgtgt ccaccaaccc gtttcgcctc ggcacccata cgcttccaga gcggatggtc
     4261 ataggcttcg aaccacggtg ccatgttttg atcccacccg tgataattcg tgcggggctt
     4321 atcgcccagc gccttgcgca ccggttcggg caaatcctcc ttgcggtagt ccagcgcgat
     4381 acgattcggg aaccccgcga aggtgccgcg cgtaccctga atcaggttat ggcggctgta
     4441 gggacgcggc gaggtctcat cccactgcac catgatc
//
LOCUS       DAOSRB010000223         4463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17666_length_4463_cov_6.745690, whole genome shotgun sequence.
ACCESSION   DAOSRB010000223 DAOSRB010000000
VERSION     DAOSRB010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17666_length_4463_cov_6.745690"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLZ74_09830"
     CDS             <1..773
                     /locus_tag="PLZ74_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98661.1"
                     /translation="WIMAFAAGHDAGVDFQFGLPKTYEKCERIRLWEDARRASAFTET
                     EKRLMRQTDTLWELKREDGKFTLSFVRRWKGERFEELPASAAPVKALGTGTAVMPRSI
                     NRKWVHASGQCERWCITDDLIAKVSVKPADFEVVSPIDNRKWAMTVPLFIMRAPENAS
                     PVVNPVTHYRWGNRRGKIRVKTTLAPGEYVTIPHRVSLAVVYNARHELLREVALEDIP
                     RASFAKGDTVGVSFTSDNTEPVPLCVNLRFIESSIEEK"
     gene            1196..1558
                     /locus_tag="PLZ74_09835"
     CDS             1196..1558
                     /locus_tag="PLZ74_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOR98662.1"
                     /translation="MDIYVGNLAYTTSDESLKVAFEAYGEVTSARVVSDRMTGRSKGF
                     GFVEMPDRTQAQAAIDALNGKELDGRTIRVNESQPKPREERGGGFRGGAGGGGGFRGG
                     SGRGGAGGRGGERRESRW"
     gene            2204..2401
                     /locus_tag="PLZ74_09840"
     CDS             2204..2401
                     /locus_tag="PLZ74_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98663.1"
                     /translation="MKQLLMIAVMAAATCAFVGCKKEESLGDKMDKAVKSAEKSANTA
                     AKDTEKAAGDLQKKLDGALKK"
     gene            complement(2473..3366)
                     /gene="galU"
                     /locus_tag="PLZ74_09845"
     CDS             complement(2473..3366)
                     /gene="galU"
                     /locus_tag="PLZ74_09845"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOR98664.1"
                     /translation="MITKAVIPAAGFGTRFLPATKALPKEMLPILDTPVIQYVVEEAV
                     ASGITDIVMVIGRGKRAVEEHFDRSFELEAELEAKGRLRELETIRAISELANIHFVWQ
                     KRMLGLGDAIRCARYHVGDQPFAVLLGDTLLQSLSGVPATRQLMEAQSRHGGCVVAVE
                     EVPRERVSQYGIISGEPLDAETIRIRDLVEKPAPDEAPSQWAVASRYILTPDIFARID
                     AATPGRNGEIQITDALRARALEGEPLFGFRVAARRHDIGNMLGFLTANIRYALGHAEL
                     AGPLGAFILAEAEAIRRARGE"
     gene            complement(3383..4378)
                     /locus_tag="PLZ74_09850"
     CDS             complement(3383..4378)
                     /locus_tag="PLZ74_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOR98665.1"
                     /translation="MIIRTTAYPRAALIGNPSDGYYGKTIAFTFSNFSARVILYESPE
                     LEIMPTRRDGSVFTSIGDLFQDVRLFGYYGGIRLLKAAIKSFYEYCRENEIELHDRNF
                     TVRYDTDIPNLVGLAGSSAIITATMRALMAFYGVTIAKPRLANLIRAVENRELGIAAG
                     LQDRVAQVYQGLVYMDFDQALMERQGFGRYEPLDVATLPPLYIAFRTDLSEGSEVVHN
                     NLHDRFERGEPEVLAAMTYWADLTAQARQALEKGEGGELGRLMNANFDKRREICAIGE
                     GNLRLVETARQVGASAKFTGSGGAIIGTYPDERTYTRLTEVFAAQQVCVFKPDIL"
BASE COUNT          882 a         1336 c         1346 g          899 t
ORIGIN      
        1 actggatcat ggcgttcgcc gccggtcacg atgcgggcgt ggacttccag ttcggcctgc
       61 cgaaaaccta cgagaagtgc gagcgcatcc gcctgtggga ggatgcccgc cgtgccagcg
      121 cgttcaccga aaccgaaaag cggctcatgc gccagacgga cacgctgtgg gaactgaagc
      181 gggaggacgg aaagtttacg ctctcgtttg tgcgcagatg gaagggcgag cggttcgagg
      241 aactgcccgc ctccgcagcg ccggtcaagg cactcggaac aggcaccgcc gtgatgccgc
      301 gctcgatcaa ccggaagtgg gtccacgcct ccgggcagtg cgaacgctgg tgcatcacgg
      361 acgacctcat agcaaaggtg tcggtaaaac cggcggattt cgaggttgtg tcgccgatcg
      421 acaaccgtaa atgggcgatg accgtgccgc tttttatcat gcgtgcgccc gaaaacgcca
      481 gtccggtggt caatccggtg acgcactacc gctggggcaa ccgccgcggc aagatccgcg
      541 tgaagaccac gcttgcgcct ggcgagtatg tgacgatccc gcaccgggta tcgctggcgg
      601 tcgtctataa cgcgcgccac gaactgctgc gcgaggtggc ccttgaggat attccgcgcg
      661 cgtcgttcgc aaagggtgac accgtgggcg tgtccttcac ctcggacaat acggagccgg
      721 ttccgttatg cgtgaacctg cgtttcatcg aatcgtccat cgaagagaaa tgagatgcct
      781 taacggaagg gacgcatgaa ggtcgacttg cgaccagcga ctcccccctc gagccatgcc
      841 ccccaaaacc accgaaaccc gaaacctcaa gcggccaggg aatcgaggga tacgccttct
      901 aaaaacgcaa ttgacattcg atccacgata cgctattttt ccctcgtcaa ttgcgaatta
      961 gctgtttgat cttgtctctg attccatggt tttcggctca tcgcgatgga ggaaacgcct
     1021 gacttcaggc gaaacgcgtt tttgatacac actccctttg gggtcgcttt agcccgttgt
     1081 tacgggccgt gcgacatagg cgaggctgcc tacgaagctg gatcagccat ggggtccggt
     1141 tgcgcctgac acacgaacac aacatggcgc gagtgaagta gagaaggata gagacatgga
     1201 catctatgtg ggcaatcttg cctatacgac gagcgatgag tccctgaagg ttgcgttcga
     1261 agcttacggc gaggtgactt cagcccgtgt tgtttccgac cgcatgacag gccgttccaa
     1321 ggggtttggt ttcgtggaga tgccggaccg cactcaggcg caggcggcaa tcgatgccct
     1381 caacggcaaa gaactggacg gccgtaccat ccgcgtgaac gagtcgcagc cgaaaccgcg
     1441 tgaagagcgc ggcggcggtt tccgcggcgg cgcggggggc ggcgggggtt tccgtggtgg
     1501 ctcgggtcgc ggcggcgcag gcggccgcgg cggcgagcgt cgcgagtccc gttggtaggt
     1561 ccgtatggat ccgctgtttg cggatccgtt tagactgtgc gaagccgttc cctaacgggg
     1621 gcggctttgt ttttaccctg ccggggaaga tcgcgacatc ggtcccgcga tcggcgtagc
     1681 cgtttcaact cttcaccttc aactctcttt ggtttctggt ttcaaggttt catggtttca
     1741 tggtttctgg gtttctgggt ttctgggttt catggtttca tggtttcatg gtttcatcgg
     1801 ggtcgggatc gctatcggga tcgggatcga tcatttaacc acggaacacc accgcctcac
     1861 agcctaacag cctcacagtc tcacagcctc acagtctaac agcctcacag tctcacagcc
     1921 tttgagattg cgggctccgc ccgcatctca atacggtgcg gcgaggaccg cgacaaaacc
     1981 caatgtttac ggggctttgc tagaattcta cccgttcacc tgcccctttt agggtaaacc
     2041 gtagtgccgt tcgtgaccgt ggagaactct gtcgggacag tgcggtgttt tgtctagaaa
     2101 atatccccat tgtcagcccc cccccgatcc tgctatagta tcgcacacac acgtaaacgt
     2161 gttaccttgc gcaatccgcg caaagaagaa ggagtacaac aagatgaagc aattgcttat
     2221 gattgccgta atggccgccg cgacatgcgc gtttgtcggt tgcaagaaag aggagtctct
     2281 gggcgataag atggacaaag ccgtcaaatc cgccgaaaag agcgccaaca ccgcagcgaa
     2341 ggacaccgaa aaggctgccg gcgatctgca gaagaagctc gatggcgcgc tgaagaaata
     2401 agcgttccgc gaatttatcg cgaaaaacgg atgctggact cccttgtccg ggcatccgtt
     2461 ttttttgtgc catcattcgc cgcgcgcgcg gcggattgct tcggcctcgg cgaggatgaa
     2521 cgcgccgagc ggcccggcca gttccgcatg gcccaacgca taccggatgt tggcggtgag
     2581 gaatcccagc atattgccga tgtcgtggcg gcgcgcagcc acgcggaagc cgaacagcgg
     2641 ctctccctcg agtgcgcgtg cccgcagggc gtcggtgatc tgaatctcac cgttccggcc
     2701 gggtgtcgcg gcgtcaatac gcgcgaaaat gtcgggcgtc aggatatacc ggctggcaac
     2761 cgcccattgc gagggcgcct cgtccggggc aggtttttcc acaaggtcgc gaatccggat
     2821 cgtctccgcg tcaagcggtt cgccgctgat gatcccgtac tggctgacgc gctcccgcgg
     2881 cacttcctcc accgccacca cgcagccgcc gtgacgcgac tgcgcctcca tgagctgtcg
     2941 ggtggccgga acgccgctca gactctgaag cagcgtgtct cccaacagca ccgcgaacgg
     3001 ttggtccccg acatggtatc gcgcgcaacg gatcgcatcg ccgaggccca gcatacgctt
     3061 ctgccagaca aagtggatgt tggcgagttc agaaatcgcg cggatcgttt ccaactcgcg
     3121 caggcgcccc ttggcctcca gttccgcctc cagttcgaaa ctgcggtcga agtgctcttc
     3181 caccgcccgc ttgccccggc cgatcaccat cacgatgtcg gtgatcccgg aggccaccgc
     3241 ttcttcgacc acatactgga tgaccggcgt gtcgaggatc ggcaacatct ctttgggcaa
     3301 ggctttggtc gccggcagaa accgcgtgcc gaaaccggcg gcgggaataa ccgctttcgt
     3361 gatcatgacg tgaacccccg tgttagagga tatctggctt gaagacgcag acctgctggg
     3421 ccgcgaacac ttcggtgagg cgcgtgtagg tacgctcatc aggataggtg ccgatgatcg
     3481 cgccgcccga cccggtgaat ttggctgagg cgccgacctg acgcgcggtt tccaccagac
     3541 gcaggtttcc ttcgccgatg gcgcagatct cgcggcgctt gtcgaaattg gcgttcatca
     3601 gccttccgag ttccccgcct tcaccctttt ccaaggcctg ccgcgcctgt gccgtgaggt
     3661 cggcccaata cgtcatcgcg gcgagcacct cgggttcgcc gcgctcgaac cggtcgtgca
     3721 ggttgttgtg gaccacctcg gagccttcgg aaagatccgt acgaaaggcg atataaagcg
     3781 gcggcagggt cgccacgtcg agcggctcgt accgtccgaa gccctgacgc tccatcagcg
     3841 cctggtcgaa atccatgtac acaaggccct gatagacttg cgccacgcgg tcttgcaagc
     3901 cagcggcgat ccccaactcc cggttctcca cggcgcggat caggttggcg agccggggct
     3961 tggcgatggt cacaccgtaa aaggccatca gcgcacgcat ggtggcggtg atgatcgcgc
     4021 tcgaaccggc gagcccgacc agattgggga tgtcggtgtc atagcgcacg gtgaagttac
     4081 ggtcatgcag ttcaatctcg ttctcgcggc agtactcgta aaagcttttg atggccgctt
     4141 tcaagagccg gattccgcca taatagccga acaggcgtac gtcttggaac aggtcgccga
     4201 tgcttgtgaa gacggagccg tcgcgccgcg tcggcataat ctccagttcc ggcgactcgt
     4261 acaggatcac ccgcgccgaa aaatttgaaa aggtgaacgc gatcgtcttc ccgtagtatc
     4321 cgtctgacgg gttgccgatc agcgcggccc gcggataggc tgtcgtgcga ataatcatac
     4381 cgtcttcctc tccggtttcc cgaaaagtct aatagtaatc ctccccgcgc aatggtacaa
     4441 gccgataatc cggattgtct tca
//
LOCUS       DAOSRB010000224         4452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17722_length_4452_cov_6.786900, whole genome shotgun sequence.
ACCESSION   DAOSRB010000224 DAOSRB010000000
VERSION     DAOSRB010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17722_length_4452_cov_6.786900"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..4260)
                     /locus_tag="PLZ74_09855"
     CDS             complement(256..4260)
                     /locus_tag="PLZ74_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOR98666.1"
                     /translation="MVSYLQRDLNSGSKTYYRIGSGNSFYGHAPFFYTREWSASQAYH
                     PKLVVTYTPPSFPDFIVESISLSNTTPVEGEEVTATVVVKNQGDASGNARYLDIWYDK
                     PSAASVGDQGDKWTDVGIIYSGNTKTKTYIFIASGSGTKTFRAFIDSQGETSESNENN
                     NQSTKTYTVVSPSPEIEVRGNNVIITDGDTTPSTTDHTDFGTTSVGSPVSRTYTVKNI
                     GTATLTTSGLSVPSGYTVTEGLSSSIPAGGQDAFTVRLSANAAGTYSGDVSFSNNDSN
                     ENPFNFRITGVVGGGGRSARRTISGTCATITVTPSAGTAAWGVEETLPSGLTPSNLTG
                     PNANWNATSRKISWYGTGASPATLGYCVSGADGTYTVSGQANFDGGANVSVTGDNTFS
                     IRPCHPADTNGDWQMTMGEAIAYLAGWQRGEYPMNYAIRAAYLWQKGPQYERRAGDEP
                     MCWEVKAAAPAPFALKALSAPRTGIQAPSGDAASRTITGTTVSITVTPSAGTAAWGFE
                     ETIPDGLTPANLSGPNANWNATSRKITWYSTGATAATLGYTVSGTDGTYSLTGLANFD
                     GADSPVSGDTQLVIGMDPDSGSAVRAISGTTVTITVTPAAGTTAWGFEETIPAGLTPA
                     NLSGPNANWNATSRKISWYSIGAAAATLGYTVSGAAGTYSLTGRLANFDGADSPVSGD
                     TQLVIGANPSGSAVRTISGTAVTITVTPPAGTAAWGFEETIPDGLTPANLSGPNANWN
                     ATSRKITWYNTGAAVVTLGYTVSGAVGTYTLTGLANFDGSDSPVSGDTQLVIGTLPPP
                     VLSVTPATRPVSKGAGSTTFDVANTGSGTMNWNAAVISGGAWARITSGASGSNAGTIT
                     IGFDANPAGGAQRTATIRVTAPGATGSPVNVRVVQEANPVDDNGGGTGDVSYLCNPED
                     DAELTTVGMYDGYLYAYRAFGEVEVPTVQGTLNVRLSNLDGRLTAKAVVQAGNVNFSG
                     KLWTDQDEDGTFYSTLTTRGGERFDLFVRQNRIWGTLTGGKFGGSEVFLEGSRTRFAD
                     RKDVMAQAILNRYKGYYTATLCGDCRPESFIQGTLNAAPRGYGYLTLTVNTGGRVRIA
                     GVLADGTSVSQSAQLLYFADCGEWLCVPFFVPLYRKTGYASGLFWIDPETREIYCGAE
                     TDEAVRWEKPGKGPDGFTGLLDMLGGPYNKSPTLQTQYEFMSAVNWDLATYHHVGGAE
                     EYTCPLNWIPVTASGLRMSMPRAGRPVRFRDAGETWYEYGGDNPTGTTLSFTARTGIF
                     RGNFTLYYDYYDARDRFQHKTVRVPYVGVMLQDPETGRLESGYGHCLFPSNDPELKAY
                     RIKPSFPVFLRGQD"
BASE COUNT          819 a         1415 c         1289 g          929 t
ORIGIN      
        1 gatcggaatc gctatcggga tcgtccgcgg acgcctcggg gatgcgtccc taccccgaag
       61 cccggcacgg tttcggcgat ccggttccgc gccgatggcg cgtagccgag gacgtccgag
      121 gaggcggaac gctgaacgtg aaaccagaaa tctcggcacc cttggcgtct gcggcgattt
      181 cggaaaaacc tccctgccag cgggcgggac acccgcgctt gtctcccgac tccatcacga
      241 aagcactttt caggatcaat cctgtccccg caggaagact gggaacgacg gcttgatcct
      301 gtacgccttc agttccggat cgttggacgg gaacaggcag tgcccgtatc ctgattccaa
      361 ccggccggtc tccggatcct gcagcattac gccgacgtag gggactctga ctgttttgtg
      421 ttggaagcgg tctcttgcgt cgtaataatc ataatacagc gtgaaatttc ctcggaatat
      481 gcccgtacgg gccgtgaaac tcagcgtcgt tccggtgggg ttgtcgcccc cgtactcgta
      541 ccatgtttcg ccagcatctc tgaatctgac aggtcggccc gctcggggca ttgacatcct
      601 gagaccggag gctgtgacgg ggatccaatt cagggggcaa gtgtactctt cggcaccgcc
      661 cacgtgatgg tacgtcgcga gatcccagtt caccgcggac atgaactcat actgcgtttg
      721 cagggtaggc gatttgttgt agggtccacc caacatatca agtagtccag tgaagccgtc
      781 agggcccttg cccggtttct cccacctcac ggcttcgtcc gtctctgcgc cgcaataaat
      841 ctcacgtgtc tcgggatcga tccagaacaa cccgctggca tacccggtct tgcggtaaag
      901 cgggacgaag aacggcacgc agagccactc gccgcaatcc gcgaagtaga gcagttgggc
      961 ggactgcgac acgctcgtgc cgtctgccag aacgcccgcg atcctgactc tcccgcctgt
     1021 gttgacggtc agcgtcaggt atccataccc acgcggtgcg gcgttcagag tcccttggat
     1081 gaaactctcg ggcctgcaat caccgcacag cgttgccgtg tagtacccct tgtaccggtt
     1141 gagaatggcc tgcgccatca cgtctttccg gtctgcgaac cgggtccgcg aaccttcaag
     1201 gaagacttcg ctgccgccga actttccacc cgtgagcgta ccccagatcc ggttctggcg
     1261 gacgaagagg tcgaatctct cgccgccccg cgtggtcagc gtggaataaa aggtcccatc
     1321 ctcgtcctga tccgtccaca gcttaccgct aaagttgacg tttccggcct gaaccaccgc
     1381 cttggcggtc agacggccgt ccagattgct gagtctgacg ttcagtgttc cttgcaccgt
     1441 cggcacctcc acctccccga acgctcggta cgcatagaga tacccgtcgt acatcccgac
     1501 tgtcgtcaac tccgcgtcgt cctcgggatt gcacagataa ctgacgtcac cggtcccgcc
     1561 accgttatca tcgacgggat ttgcctcctg caccaccctc acgttgaccg gacttcccgt
     1621 cgcaccgggc gctgtcacgc ggattgtcgc ggtgcgttgc gccccgcctg cgggattcgc
     1681 atcgaatccg atcgtgatcg tgcccgcgtt gctgccgctc gcgcctgagg tgatccgtgc
     1741 ccatgcgcca ccagaaatca ccgccgcatt ccagttcatc gtgcccgatc ccgtgttcgc
     1801 cacatcgaag gtagtactgc ccgccccttt cgatacagga cgggtggcgg gggtgacgga
     1861 caggacgggt ggcggcagcg tcccgatgac gagctgcgta tcgcccgaca ccgggctgtc
     1921 ggagccgtcg aagttcgcga gccccgtgag ggtgtacgtg ccgaccgcac cggacacggt
     1981 gtagcccagc gtcaccaccg ccgcgccggt gttgtaccag gtgatcttgc gcgaggtcgc
     2041 gttccagttc gcgttcggcc cagacaggtt ggcaggggtc aggccgtcag ggatcgtttc
     2101 ctcgaagccc cacgccgccg tgcccgcagg cggcgtcacc gtgatcgtga ccgccgtgcc
     2161 gctgatcgtg cgaaccgcag atccactggg gttcgccccg atgacaagct gcgtatcacc
     2221 cgacaccgga ctgtcggcac catcgaaatt cgcgagccgc cccgtaaggc tgtatgtgcc
     2281 ggccgcgccg gacacggtgt agcccagcgt cgccgccgcc gcgccgatgc tgtaccaaga
     2341 gatcttgcgc gaggtcgcgt tccaattcgc attcgggccg gacaggttgg ccggagtcag
     2401 gcctgcgggg atcgtctcct cgaagcccca cgccgtcgtg cccgcagcag gcgtcaccgt
     2461 gatcgtgacc gtcgtgccgc taattgctcg aaccgccgag ccactgtcgg ggtccatccc
     2521 gataacgagc tgtgtatcgc ccgacaccgg actgtcagca ccgtcgaagt tcgcgagccc
     2581 tgtgaggctg tatgtgccgt ccgtaccgga cacggtgtag cccagcgttg ccgccgttgc
     2641 gccggtgctg taccaggtga tcttgcgtga ggtcgcgttc cagttcgcgt tcgggccgga
     2701 caggttggcc ggagtcaggc cgtcgggaat cgtctcctcg aaaccccacg ccgccgtgcc
     2761 cgccgacggc gtcactgtga tagacaccgt ggttccggtt attgtccggc ttgcggcatc
     2821 gccgctggga gcctgaatgc cggtacgcgg cgcactcaac gctttcagcg cgaaaggtgc
     2881 gggtgccgcc gctttcactt cccagcacat gggctcgtca cctgcacgac gttcatactg
     2941 tggccctttt tgccacaggt acgccgcgcg gatcgcatag ttcatggggt actcgccccg
     3001 ctgccaccca gcgaggtacg caatcgcttc gcccatcgtc atctgccagt cgccgttcgt
     3061 atcggcggga tggcagggac ggatgctgaa agtgttatca cccgtgaccg acacattcgc
     3121 tccgccgtcg aaattcgcct gaccgctgac cgtgtatgtc ccgtcggcac cggacacaca
     3181 gtaccccaac gttgcgggtg atgccccagt gccgtaccag gatatcttcc gagaggttgc
     3241 attccaattc gcgttggggc cggtgaggtt ggacggtgta agcccggagg gcagggtctc
     3301 ctcaacaccc cacgccgcag tgccagcgga tggagtcacc gtaatagttg cacaggttcc
     3361 cgaaatcgtg cgtcgagcac ttctgccgcc gccgccgacc acgcccgtga tccggaaatt
     3421 gaacgggttc tcattggagt cgttattact aaagctgaca tctcccgagt acgtacccgc
     3481 agcgttcgcg ctcaggcgca ccgtgaaagc atcctgcccg ccagccggaa tactcgaaga
     3541 gagtccttcc gttaccgtgt atccgctcgg caccgacaga ccagaggtgg tcaacgttgc
     3601 cgtaccgatg ttcttcacgg tataggtgcg gctgaccggc gagcccaccg aggtcgtccc
     3661 gaagtccgtg tgatccgtcg tcgaaggcgt tgtgtccccg tccgtgatga tcacgttgtt
     3721 gccgcggacc tcgatctcgg gcgatggcga tacaaccgta taggtcttcg tgctctggtt
     3781 gttattttca ttactttcgc tcgtctcacc ctgactgtcg atgaaggcgc ggaacgtctt
     3841 tgtcccagag cccgatgcta taaagatgta cgttttagtc ttggtgttgc cggaatagat
     3901 tatacccaca tcagtccatt tatcgccttg gtcaccgaca ctggccgcag aaggcttgtc
     3961 ataccagatg tcaagatatc tggcgttgcc cgacgcgtcg ccctgattct tcaccaccac
     4021 ggtggccgtg acctcctccc cctcaaccgg agtcgtattg ctcaaggaaa ttgactcaac
     4081 aatgaaatcg gggaatgatg ggggcgtata agtaacaaca agtttcggat gatacgcctg
     4141 tgaggccgac cattcgcgcg tgtaaaaaaa cggagcatga ccgtagaaag aattccccga
     4201 gccaattctg taatatgtct tggagccact attcaggtct ctctggagat agctcaccat
     4261 agctccaacg gaatcaaata cccactgtcc gttgctgggt ctactaacat caccgtggtt
     4321 cccgtaggtt ccatcaggca atagggccga gggcctcgtg ttccaagtaa ggctgttctc
     4381 actgaacggt cgggacgaga agatcgtcag actataacca agatcttctg gttttgtaaa
     4441 ctcatagagt tt
//
LOCUS       DAOSRB010000225         4425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17842_length_4425_cov_8.353089, whole genome shotgun sequence.
ACCESSION   DAOSRB010000225 DAOSRB010000000
VERSION     DAOSRB010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17842_length_4425_cov_8.353089"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(647..1171)
                     /locus_tag="PLZ74_09860"
     CDS             complement(647..1171)
                     /locus_tag="PLZ74_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOR98667.1"
                     /translation="MNLLLHTCCAPCASHCVLTLRELGHTVTLFYTNANIAPHAEFLR
                     RLESVERLAERLAVPLLVEEPDHADWLARVACGLEDASERGPRCAHCFRYSLCRTAAR
                     MRQCGFDGFTTSLSVSPHKHTATLFEAGRAIDAERFLAIDFKKRDGFLRSLQLATEFG
                     LYRQSYCGCEFSRN"
     gene            complement(1168..2415)
                     /locus_tag="PLZ74_09865"
     CDS             complement(1168..2415)
                     /locus_tag="PLZ74_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOR98668.1"
                     /translation="MPRPYYADLHVHSHYSRATSPACTPEGLFRWARLKGVGVCGTGD
                     CTHPAWLTELRAKLIEDAPGLFTLKPALRGEACSGVPEACRGPVRFMLTGEISSIYRR
                     DGACRKIHSLVLLPSFEAAAALNERLAQVGNILSDGRPILGLDPRDLLTMLLECDPRA
                     ALIPAHIWTPWFSLLGAKSGFDSPEACFGDLMPHLFAVETGLSSDVPMNRRIRCLDRL
                     TLVSNSDLHSPAHLGRNATVFYGEPAYDTILKGLRTADPALYGGTVDLFPEVGKYFLD
                     GHRACRVRLTPEESLACGTLCPACGKPVTIGVLHRVVELERRQAASARSRAFRTPYRY
                     IVPLPELLSQQLGTAPRSKKVGTAYQALLAQVGPELPLLLDADARTLGALDALGEAIR
                     GVREGRVRREGGYDGEYGSVRVI"
     gene            complement(2462..3997)
                     /locus_tag="PLZ74_09870"
     CDS             complement(2462..3997)
                     /locus_tag="PLZ74_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOR98669.1"
                     /translation="MLARVCSGAVYGVHAYTVEIEVNAGRGDPQFVMVGLPDAAVRES
                     KDRVWTAMNNSGFRPTLGRTTINLAPADIRKEGPVYDLPIAAGLLAATGEAELPHLDQ
                     YALIGELALSGEVRRVRGVLPVTLEMRRQGKRGILVPADNAEEAAVAEGIDVYPVRHL
                     REAVDFLAGKLSLHPFRVDLAGLMRRERRHAEDFADVKGQETAKRAIEVAVSGGHNIL
                     MIGSPGAGKTMLARRIASILPEMTLDEAVEATRIHSVAGTLKSHQALILHRPFRAPHH
                     TISDAGMLGGGTHPLPGEVSLAHRGVLFLDELPEFHRNVLEVLRQPLEDGHVTISRAA
                     ATLDFPSQFILVAAMNPCPCGYAGDPKRECRCSSQKIQAYRNKISGPLLDRIDIHIEV
                     PPVRYADLSGLARGDASAVIRERVAVCRARQRERFRELPRLTCNAAMGSREVQTFCPL
                     SGDAAELLKMAMTELNFSARAYDRIIKVARTIADMAGSEIIGPNHISEAIQYRSLDRL
                     MWV"
BASE COUNT          918 a         1467 c         1369 g          671 t
ORIGIN      
        1 ccggcatcga gcgcgcctac ctccagcgcc tgatccgcaa gtacggcatg cgcgagtgag
       61 ccgggcaggg acgcatcccc gaggcgtccg cggacggttc ggggaaccgt ccctaccagc
      121 gggcggggac gcccgcgctc ccgactttcc cgcgccagta tcgatcccga tagcgttccc
      181 gatcccgatc ccgatagcga tcccgacccc gatttagatg aaaccctgaa accagaaacc
      241 ttgaaaccct gaaacccgat cccgaccccg atttggatga aaccctgaaa ccttgaaacc
      301 ctgaaaccct gaaaccagaa accctgaaac cagaaaccca gaaaccagaa acccagaaac
      361 ccagaaaccc agaaaccaga aaccctgaaa cccgaaaccc agaaaccaga aaccttgaaa
      421 agcgacctgc ggtgtcggaa acgcgggcgt ccctgtcgat cgccgcaggt cgaatgtcgc
      481 gggtcgcggg tcgacgttcc cgatagcgat accgatcccg accccgaccc cgatttagat
      541 gaaacccaga aaccagaaac cctgaaaccc agaaaccaga aacctgaaac ccagaaaccc
      601 tgaaaccctg aaacccgatc ccgatcccga tagcgatggg atcggtttaa ttgcggctga
      661 actcgcagcc gcaatacgac tggcggtaca gcccgaactc cgttgccaac tgcagactgc
      721 gcagaaaccc atcgcgcttt ttgaaatcga tcgcgagaaa ccgctcggca tcgatcgcgc
      781 ggcccgcctc gaacagcgtg gccgtgtgtt tatgcggact cacggacagc gaggtggtga
      841 acccgtcaaa cccgcactgc cgcatgcgcg cggcggtccg gcagagactg tagcggaaac
      901 agtgcgcgca gcggggtccc cgctcggacg cgtcctccag cccgcaggca acgcgggcca
      961 gccagtcggc atggtccggc tcctccacca gcaacggcac ggcaagccgt tccgccagac
     1021 gctccaccga ctccagacgg cgcagaaact ccgcgtgcgg cgcgatgttc gcgttggtgt
     1081 aaaagagggt caccgtatgc ccaagctccc gaagcgtcag gacgcagtga ctcgcgcagg
     1141 gcgcgcagca ggtgtggagc agcaggttca tatcacgcgc acgctcccgt attcgccgtc
     1201 atacccgccc tcccggcgca cccggccctc acgcacgcca cggatggctt cgcccagcgc
     1261 gtcgagggcg ccgagagttc gcgcatcggc atccagcagc agcggcagtt cgggtccgac
     1321 ctgcgcgagg agcgcctgat acgcggtgcc gacttttttg gaacggggtg ccgtgccaag
     1381 ctgctgcgac agcagttcgg gcagcggcac aatgtaacgg tagggtgtgc ggaaagcgcg
     1441 ggagcgggcc gatgcggcct gacgccgctc caattccacc acccggtgaa gcacgccgat
     1501 ggtgaccggt ttgccgcaag ccggacagag ggtgccgcag gcgaggctct cctccggagt
     1561 caggcgcaca cggcaggcgc ggtggccatc gaggaaatat ttccccacct cgggaaaaag
     1621 atccaccgtg ccgccgtaca gcgcgggatc agcggtgcgc agccccttga ggatcgtgtc
     1681 ataggcaggc tcaccgtaga agacggtggc gttgcgtccg agatgggcgg gcgaatgcag
     1741 gtccgagttc gacaccagcg tcagccgatc gagacaacgg atgcgccggt tcatcggcac
     1801 atccgaggag aggccggtct cgaccgcgaa caagtgcggc atgagatcgc cgaaacacgc
     1861 ctccggcgaa tcgaatcccg atttcgcgcc gagcagggag aaccacggcg tccagatgtg
     1921 cgccggaatc agggccgcgc gcggatcgca ctccagcagc atcgtcagca ggtcgcgggg
     1981 atccagaccg agaatgggcc gtccgtcgga aaggatgttg ccgacctgcg cgagccgttc
     2041 gttcagcgcg gcggccgcct cgaaagaggg cagcagcacc agactgtgga ttttgcggca
     2101 cgcgccatcc cggcggtaga tgctgctgat ctcgccggtc agcatgaagc gcaccggacc
     2161 gcggcaggcc tccggcacac cgctgcaggc ctccccgcgc agggcgggct tgagcgtaaa
     2221 gaggccgggc gcatcctcaa tcaacttggc gcgcaactcc gtcagccagg cggggtgtgt
     2281 gcagtccccg gtgccgcaga cccccacccc cttgagccgg gcccagcgga aaaggccttc
     2341 cggcgtgcac gcgggactgg tcgcgcgcga ataatgcgag tggacatgca gatcagcgta
     2401 atatgggcga ggcataggcg gcatgggttc ggttctttct tgcatggggc gggagattga
     2461 ctcagaccca catcagccgg tcgagcgaac ggtactggat cgcttcggag atgtgattcg
     2521 ggccgatgat ttcactgccg gccatatcgg cgatggtgcg cgcaaccttg atgatgcggt
     2581 cataggcgcg cgcgctgaag ttcagttcgg tcatcgccat tttaagcagc tccgcggcat
     2641 cgccgctcag cggacagaag gtctggacct cgcgggagcc catggcggcg ttgcacgtga
     2701 ggcgcggcag ttcccggaac cgttcgcgct ggcgcgcgcg gcagaccgcc acacgctcac
     2761 ggatcaccgc cgaggcgtcg ccccgcgcga gcccgctgag atccgcatag cggaccggcg
     2821 ggacttcgat atggatgtcg atgcggtcca gcagcggacc cgaaattttg ttgcggtacg
     2881 cctgaatctt ctgcgaggag cagcggcact cgcgtttcgg atcgcccgcg tacccgcagg
     2941 gacaagggtt catcgcggcc acaagaatga attgcgaggg aaagtcgagg gtggcggccg
     3001 cgcgcgagat cgtgacgtgg ccatcctcga gcggctgacg caacacctcc agcacgttgc
     3061 ggtggaactc cggcagctcg tcgagaaaca gcacgccgcg gtgcgcgaga ctcacctcgc
     3121 cgggaagcgg atgcgtgccg ccgcccagca tccccgcgtc ggagatggtg tggtgcggcg
     3181 cccgaaaagg acggtgcagg atcagagcct gatgactctt gagggtgccc gccacactgt
     3241 ggatgcgcgt ggcctccacc gcctcgtcga gagtcatctc cggcaggatc gaggcgatgc
     3301 gccgcgcaag catggtcttg cccgcgcccg gcgacccgat catcagaatg ttgtgcccgc
     3361 cgctcaccgc cacctcgatg gcgcgcttgg cggtctcctg ccctttgaca tccgcgaaat
     3421 cttcggcgtg gcggcgctcg cgacgcatga gtccggcaag gtcgacccgg aagggatgca
     3481 ggctcagttt gcccgccaga aaatcgacgg cctcacgcag atgccgcacc ggatacacat
     3541 cgatgccctc ggccactgcg gcctcctcgg cattgtcagc gggcacgaga atgccgcgct
     3601 tgccctgacg gcgcatttca agggtgaccg gcaacacacc gcgcacacgc cggacctcgc
     3661 cgctgagcgc gagttcgccg atcagggcgt actggtcgag atgcggaagt tccgcctcgc
     3721 ccgtggcggc cagcaggccg gccgcgatcg gcaagtcata gaccggcccc tctttccgga
     3781 tgtccgcagg cgcgaggttg atggtggtgc ggccgagcgt cggccggaaa cccgagttgt
     3841 tcatggcggt ccagacgcgg tctttgctct cccgcaccgc cgcgtccggc agacccacca
     3901 tcacgaactg cggatccccc cgcccggcat tgacctcgat ttcgacggta tacgcatgaa
     3961 ccccatagac agcgcctgaa cagacacggg caagcatgag ccactccttt acgcgagaat
     4021 gagacgtcag tgtaatctca tcccctgaca gacgcaaccc ccgaaaagcg ggacctctct
     4081 tggtttgccg gaagcgcggg catccccgcc gatcgccgcg ggtcgaatgt cgcgggtcgc
     4141 gggtcgacga taccgatagc gataccgata ccgataccga ccccgacccc gatttggatg
     4201 aaaccatgaa acacatgaaa cttcatgaaa atcaggaatt ccccctggcg ttcccaaaaa
     4261 cccgagcagt gccatcccgt gtgttcgtgg ttaaacaatc gatcccgata ccgatcccga
     4321 tcccgacccc gatttggatg aaaccctgaa accctgaaac ccagaaacca gaaaccccga
     4381 aaccatgaaa ccctgaaacc aaagagcgtt gctttcaacg ctctt
//
LOCUS       DAOSRB010000226         4418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_17871_length_4418_cov_6.910841, whole genome shotgun sequence.
ACCESSION   DAOSRB010000226 DAOSRB010000000
VERSION     DAOSRB010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17871_length_4418_cov_6.910841"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PLZ74_09875"
     CDS             complement(<1..674)
                     /locus_tag="PLZ74_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOR98670.1"
                     /translation="MATLIDPSTLKITDVRFADIDGAPKRCTLIKVYTNQGLIGYGEV
                     RDASSRTYAAMLKSRILGENPCNVEKIFRRIKQFGGHSRQAGGVCGIEVALWDLAGKA
                     WGVPVWQLLGGKFRDSARIYCDTDSEGGGRDMGKALKERMAQGFTYLKMDLGIELLMD
                     KPGCLSAPGGFLETMRHHKETVFSTPHGSIDPRAMRGEAYDLFSTAHPFTGIHITEQG
                     LDFLEQ"
     gene            899..1507
                     /locus_tag="PLZ74_09880"
     CDS             899..1507
                     /locus_tag="PLZ74_09880"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOR98671.1"
                     /translation="MRIYIDFDDVLCETARPLAALAGELFQSTVPYEAITGFDLQQAF
                     GLTDAEIALLMERAHQTAFLEALPPAPGGLQGVRELETRGHDLMIVTGRPASSHSGSC
                     GWLRKHGLAHLELLYVDKYGRASGQPTPDTPRTLSREEFADMSFDVAIDDSPAALDLL
                     APRRDCTVIVYDRPWNRHYAHRPNMRRTGSWREIVSLIGDLA"
     gene            1603..2073
                     /locus_tag="PLZ74_09885"
     CDS             1603..2073
                     /locus_tag="PLZ74_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98672.1"
                     /translation="MKNWTEEFDRLLPLLGHRNWILVTDQAYPYQSAPGITYFDTRAP
                     LPEVLARVATSLRNSAHVKPIFYTDLELGALTEELVPGIDAFRAATRAVLPADGVQTL
                     PHESVFAKLDAASKLFHVVVLKTDCVIPYSSVFIELDCAYWDADREQALRARLR"
     gene            2129..3532
                     /locus_tag="PLZ74_09890"
     CDS             2129..3532
                     /locus_tag="PLZ74_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98673.1"
                     /translation="MDITQKGSFIMRHAFGFSRRRFLASSAALATFTIPRRSLFGQTL
                     NAPPGKQFRFAKIGCGGMGGADLNSTVSSGGIVVGLCDVDPKRAEKAYQSYPDVPKFT
                     DYRKMLDKLEKEIDGVVISTPDHTHACAALDAMRRGKHVYVQKPLARTYEECRLLLEA
                     TRKYKVVTQMGNQGHAGPGLILWKKMMDAKAFGEIEQVHTWSDRPIWPQGMTEKPKED
                     PVPEGFDWDSWIGPASMRPFSTAYVPFKWRGWWDFGCGAIGDMACHNMDPAFWILQLG
                     MPAAIRAEASSPAGIAYPAWSIIEFTFPATPVCPKGVKVTWYDGKKLPTLPANAHPEL
                     KPGTNGCLIIGSKMSVMGGSHAAPPMPIALGGQAYGPAVKDAERHWREELKQTKGCSH
                     YGQWVEACKENDMTRPGSQFEYSVPMTQTLLLGCIALRYPGVELKWDSARNRFSNHEE
                     ANRWLADAPRAGFDLKV"
     gene            3679..4323
                     /locus_tag="PLZ74_09895"
     CDS             3679..4323
                     /locus_tag="PLZ74_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98674.1"
                     /translation="MAALKLDMGAEMGRGWRLFRANMGMLVLAGLICTVVGALTCGIL
                     AGPLFAGLLLVLDRLIREDPVRPQAGDVFKGLDFFMQALVLSLICFVISFVLSLVPVL
                     GHLAGLLVGSVLMWSMMRIVYRRETAVEALKAVGGDIGRGDFTMPFLFGALVSLIGGL
                     GFVACGVGIFFTLPLSYCMLVCSYHTLYESEGGVTTEAEMLSAEPPPPPDDLRL"
BASE COUNT          748 a         1430 c         1403 g          837 t
ORIGIN      
        1 tactgttcga ggaagtcgag cccctgctcg gtgatgtgga tgccggtgaa ggggtgggcg
       61 gtgctgaaga ggtcgtacgc ttcgccgcgc atggcgcgcg gatcgatcga gccgtgcggc
      121 gtgctgaaaa cggtctcctt gtggtggcgc atggtctcga ggaacccgcc gggcgcgctg
      181 aggcagccgg gcttgtccat gagcagctcg atgccgagat ccatcttgag atacgtgaaa
      241 ccctgcgcca tgcgctcctt gagagccttg cccatgtcgc gtccgccgcc ttcggagtcg
      301 gtgtcgcagt agatgcgcgc gctgtcgcgg aacttgccgc cgagaagctg ccacaccggc
      361 acgccccacg ccttgcccgc caagtcccag agcgcgacct cgatgccgca gaccccgccg
      421 gcctgacgcg agtggccgcc gaactgcttg atgcggcgga agatcttctc cacgttgcag
      481 ggattctcgc cgaggatgcg gctcttgagc atcgcggcgt aggtgcggct cgaagcgtcg
      541 cgcacctcgc cgtagccgat caacccttgg ttggtgtaga ccttgatgag cgtgcaacgc
      601 ttgggcgcgc cgtcgatatc ggcgaaacgg acgtcagtga ttttcagcgt ggacgggtcg
      661 atcaaagtgg ccatattttt ctcctgatgt tccagaaccc ataggataac ggttttttcg
      721 gatgcctgac taccggaaag tgcggcattc agggtccgaa acccggtccg cgcccccgcg
      781 ttcctgccga tggtcacggg gcgcatgtcg caagtccccg ttcccgagcg ttgcgcgttc
      841 tcttttttgg cgcagtccgc ggctttgcgt aaccggcgta ttgtgctatg gtacgggtat
      901 gcggatctac atcgacttcg acgacgtgtt gtgcgagacc gcgcgtccgc tcgcggcgct
      961 ggcgggtgag ttgttccaga gcaccgtgcc gtacgaagcg atcaccgggt tcgatctcca
     1021 gcaggcgttc ggcctcacgg atgcggaaat cgccctgctg atggagcgcg cgcaccagac
     1081 ggcgttcctc gaggcgctcc cgcccgcccc gggcggtctg caaggggtcc gcgaactgga
     1141 gacgcgcggc catgacctca tgatcgtcac cgggcgccct gcctccagcc acagcggatc
     1201 gtgcggatgg ctgaggaaac acggcctcgc gcacctcgag cttctgtatg tcgacaagta
     1261 cgggcgcgcc tcgggacaac cgacgccgga cacgccccgg accctcagcc gcgaggagtt
     1321 cgccgacatg tcctttgatg tcgcgatcga cgattcgccc gcggcccttg atctgctcgc
     1381 gccgcgccgc gactgcacgg tcatcgtcta tgaccggccg tggaaccggc actacgccca
     1441 ccggcccaac atgcgccgca ccggctcgtg gcgcgagata gtcagcctga tcggagacct
     1501 cgcatgaaaa ttcccctttc cgcgctgttt gcgtcagcct gtgtcctttt cggcacgggg
     1561 tgcgcggact ccgcatccac ccgcaacccc tcaggagaac gcatgaagaa ctggacagag
     1621 gaattcgatc ggctgctgcc gttgctcggc caccgcaact ggatcctggt cacggatcag
     1681 gcctacccct atcaaagcgc gccgggcatc acctattttg acacccgcgc gccgttgccc
     1741 gaggttctcg cccgcgtggc gacctccctc cggaactccg cgcatgtgaa gcccattttt
     1801 tacaccgacc tcgaattggg ggctctcacc gaggagctgg ttccgggcat cgacgcgttc
     1861 cgcgccgcga cccgtgcggt gcttcctgcc gacggggtcc agaccctgcc gcacgaatcg
     1921 gtcttcgcga aactcgacgc ggcctcgaag ctcttccacg tcgtggtgct gaaaaccgac
     1981 tgcgtgatcc cgtattcgtc ggttttcatc gagctggact gcgcctactg ggatgcggac
     2041 cgggagcagg cgttgcgcgc ccgcttgcgt tgatttgtgg cttgcccccc tgccgcatcg
     2101 ttattacact atacccgata acggtttcat ggacatcaca cagaaaggat ccttcatcat
     2161 gcgtcacgcc tttggtttca gccgccgccg tttcctcgcc tcttccgccg cgctcgcgac
     2221 gttcacgatc ccccgccgct ccctcttcgg ccagaccctc aacgcgccgc cgggcaagca
     2281 gttccggttc gccaaaatcg gttgcggcgg catgggcgga gccgatctca acagcacggt
     2341 ctcctccggc ggaatcgtgg tcggcctctg cgacgtggac cccaagcgcg cggagaaggc
     2401 gtaccagagc tatcccgacg tgccgaagtt cacggattac cgcaagatgc tggacaagct
     2461 ggaaaaggag atcgacggcg tggtgatctc cacgccggat cacacgcacg cctgcgcggc
     2521 tctggacgcg atgcgccgcg gcaagcacgt gtacgtgcag aaacctctgg cccgcaccta
     2581 tgaggagtgc cggctgctgc tggaggccac gcgcaaatac aaggtggtca cccagatggg
     2641 caaccagggg cacgccggtc cgggcctgat cctgtggaaa aagatgatgg acgccaaggc
     2701 cttcggcgag atcgagcagg tgcacacctg gtccgaccgg ccgatctggc cgcagggcat
     2761 gaccgagaag cccaaagagg acccggttcc cgaagggttc gactgggatt cgtggatcgg
     2821 tccggcctcc atgcgcccgt tctcgaccgc ctacgtgccg ttcaagtggc gcggctggtg
     2881 ggatttcggc tgcggcgcga tcggcgacat ggcctgccac aacatggacc ccgccttctg
     2941 gatcctgcag ctcggcatgc ccgccgcgat ccgggccgaa gcctcctcgc cggccggcat
     3001 cgcctacccc gcctggtcga tcatcgaatt caccttcccg gccacgccgg tctgccccaa
     3061 gggcgtgaag gtcacctggt acgacggcaa gaaactgccg accctgcccg cgaacgcgca
     3121 tccggaactg aaacccggca ccaacggctg cctgatcatc ggttccaaaa tgtccgtcat
     3181 gggcggttcg cacgctgcgc cgcccatgcc gatcgcgctg ggcgggcagg cctacgggcc
     3241 cgcggtcaag gacgccgagc gccactggcg cgaagagctg aaacagacca agggctgcag
     3301 ccattacggg cagtgggtcg aggcgtgcaa agagaacgac atgacccggc cgggaagcca
     3361 gttcgaatac tccgttccca tgacccagac gctgctgctc ggctgcatcg cgctgcgcta
     3421 tcccggcgtg gaactgaagt gggattcggc ccggaaccgg ttctcgaacc atgaggaagc
     3481 caaccgctgg ctggccgacg ccccccgcgc cggcttcgat ctcaaggttt gagttgagcc
     3541 ctcaacccga tttcatctca cacaaaggca caaaggatgc aaaggaaggg gtttcatgac
     3601 aacattccct tttagggcct tcgtggcttc gtgtgataaa caacacacac gtgccgtggg
     3661 cacagaatgg agtgggatat ggcagcgttg aaactggata tgggcgcgga gatgggcagg
     3721 ggatggcgcc tttttcgggc aaacatgggg atgctggtcc tcgcgggcct gatctgcacg
     3781 gttgtgggtg cgctgacctg cggcattctg gccggaccgc tcttcgcggg cctgctgctc
     3841 gtgctggacc gtctgatccg ggaagatccg gtccgtccgc aggccgggga tgtgttcaag
     3901 ggcctcgact tctttatgca ggccctggtg ctgagtctga tctgtttcgt gatctccttt
     3961 gtcctgtcgc tcgtgccggt gctcggacac ctcgcgggcc tgctggtggg gtcggtcctg
     4021 atgtggtcga tgatgcgcat cgtgtaccgg cgcgagacgg ccgtggaggc cctgaaggcg
     4081 gtcggcggcg atatcgggcg gggcgacttc accatgccgt tcctgttcgg cgcgctggtc
     4141 agcctgatcg gcggactggg ctttgtcgcc tgcggcgtcg gcatcttttt caccctgccg
     4201 ctgagctact gtatgctggt ctgcagctac cacaccctct acgaaagtga agggggcgtc
     4261 accacagaag ccgaaatgtt gtccgcggaa ccgccgccgc cgccggacga tctgcggctg
     4321 tgacccaatc gcacggacgc ctcggggatg cgtccctacc gggaacctaa aaagagcgtt
     4381 gaaagcaacg ctctttggtt tcagggtttc ggggtttc
//
LOCUS       DAOSRB010000227         4376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_18075_length_4376_cov_8.570007, whole genome shotgun sequence.
ACCESSION   DAOSRB010000227 DAOSRB010000000
VERSION     DAOSRB010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18075_length_4376_cov_8.570007"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..969
                     /locus_tag="PLZ74_09900"
     CDS             124..969
                     /locus_tag="PLZ74_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOR98675.1"
                     /translation="MWLLIAASFLFSFSFGLIGNRLAGLPPAWLAAARLTVAAAVFLP
                     FARRVPFRTGAALFAVGMVQFGLMSLTYMASFRFLASHEVALFTVMTPLYVTVLHDLH
                     RRAFHPVNLLAASLAVAGAALILWRGRLSGAPLTGFLLVQASNLCFAAGQIAYRALTS
                     AARAPLSDRQVFFWLYLGGLTALLPFGLRDALLLAPRPTLTQLAVLLYLGVAVSGLSY
                     FLWNAGARRVSSGVLAVMNNIKIPLGMLVSLTCFGEPVQHASLLAGGTLILAALLPPL
                     LRRKA"
     gene            complement(1033..1635)
                     /gene="lpoB"
                     /locus_tag="PLZ74_09905"
     CDS             complement(1033..1635)
                     /gene="lpoB"
                     /locus_tag="PLZ74_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HOR98676.1"
                     /translation="MNKNWIGMAGVAAAAVMMTGCATTPTVREVALDRAPITSKLEPQ
                     DVRRTVEKMAESLLSAPGVREAVGNTRPVLDVEPLKNRTTQHVDMVSITDSLRMQLLR
                     SGLFRFVDKSTSGTDIEFMDSQANLGLTDQRKAIKPGQQSAAQMYLTGALTEIKNQVG
                     RTVDQYYKFSMILKDLRSGELVWADEQEIRKESVKPRVGF"
     gene            complement(1665..2087)
                     /locus_tag="PLZ74_09910"
     CDS             complement(1665..2087)
                     /locus_tag="PLZ74_09910"
                     /inference="COORDINATES: protein motif:HMM:NF018889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HOR98677.1"
                     /translation="MKRINGLLGAVALAAGLTALTGCVETAGTVVTTDHTTGSTVVLE
                     DSTRLRGQVQVMKVNYDEVNGLKRVHITLASTRHRRLRLHYRIIWFDANGMELDPDTK
                     TYRTLILEGRDAVTVTGVANSTAGVTSKLRVRDLEAAD"
     gene            complement(2159..3679)
                     /locus_tag="PLZ74_09915"
     CDS             complement(2159..3679)
                     /locus_tag="PLZ74_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98678.1"
                     /translation="MRNTIPCSAGCGALLLLVAAGCVSPNVSTDRALASLRAGDDPQA
                     LEWSEKLKRSFYSKDLGYAETGRVRMLSGDFHGSSTNFSVLIDKVIEDTESGPVIKMG
                     SVGANVMAGTITDDRTRSYHLPAYEFINALQYQLLNHIFLGAPDAAAVEARRAVFAQE
                     QIAEKYGKEVQEIQAQAADGKHAKASQTVDAQMQSMAPVLELSRSSFENGLTWYLCGL
                     LFEQDQDQANASLSYRKAWELMPGNPYIQKDFLRLLRTQDAEAYKGLLAQTGTDPATL
                     KRDATEIILILEEGFISQRQSMKVPLPVPGVNTVVSVDFPVYRDDLYAPSTIAIRGGG
                     RELGMIALALSVQSLAYRDLKEKIPGIVVRNATRVATRVAAQYGANQAGDLAKYSVMA
                     FNAVSAVINKADTRAWYTLPVVSHLYRGGVEPGEHVIELRNQANGLVTRVPVKVAEGE
                     TRLIWVADFGGNARAATASLNGKGAPATFRVCGSLLAGFPPVTLPGGPRTLGARQP"
     gene            complement(3750..>4376)
                     /locus_tag="PLZ74_09920"
     CDS             complement(3750..>4376)
                     /locus_tag="PLZ74_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOR98679.1"
                     /translation="SSVYGSNWTNKLPVPGQYTDGGDAMNYYEDLFDQWSTATGEEIA
                     GKVATWRRAAPVDTALVEFSVSSTVDELIQGLESGVRAPNELLDEMRRLVGGVTVELD
                     GATVGSSPGTFKATPGLHQLRVSRQWMRPWQQTVNIQQGATFRVALELSDAGLQRYRS
                     LEGFRAAVAIGYAEAVMRKGVKINFDTAAWRDVGNKGSEINLQQNAIQ"
BASE COUNT          701 a         1592 c         1215 g          868 t
ORIGIN      
        1 cccctcacag cctcaccccc tcaccccctc acagcctcac cccctaaccc ctcacccctt
       61 ctggccgtcc gcgccgatca aatggactcg ccgcgaccgc cgcgcttttg atacaaacgg
      121 ggcatgtggc tcctgatcgc ggcatcattc ttgttcagtt tctcgttcgg actgatcggt
      181 aaccgtctgg ccgggctccc gcccgcctgg ctggccgccg cgcgcctaac cgtggcggct
      241 gcggtcttcc tgcccttcgc ccgccgcgtg ccgttccgaa ccggtgccgc cctcttcgcg
      301 gtcggcatgg tccagttcgg cctgatgtcc ctcacctaca tggccagctt ccgctttctc
      361 gcctcgcacg aggtggcgct cttcacagtc atgacccccc tctacgtgac ggtgcttcac
      421 gatctgcacc ggcgcgcttt ccatccggtc aacctgttgg ccgcatcgct cgcggtggcg
      481 ggagcggctc tgatcctgtg gcgcggccga ctgtcaggcg cgccgctgac cggttttctg
      541 ctggtccagg cttcgaacct ctgtttcgcc gcgggccaga tcgcctaccg tgcgctgacc
      601 agcgcggcac gcgcgccgct ttccgaccgt caggtgtttt tctggctcta cctcggcgga
      661 ctgaccgccc tgctgccgtt cggcctgcgc gacgcgctgc tcctcgcccc gcgccccacc
      721 ctgacgcaac tcgcggtgct gctctatctg ggcgtggcgg tctccggcct cagctatttt
      781 ctttggaatg ctggagcgcg gcgcgtttca tccggcgtgc tggccgtcat gaacaacatc
      841 aaaatcccgc tcggcatgct ggtctcgctg acgtgtttcg gcgaacccgt gcaacacgcc
      901 agcctgctcg cgggcggcac gctgattctc gccgcgctgc tccctccgct cctccgccgc
      961 aaggcctgaa cgcgcggcac gcggccgcac ccctgcggcc gcgccacaac ccgtcatgcc
     1021 ggacggatcc gctcagaagc cgacacgcgg cttgacgctc tccttgcgga tctcctgctc
     1081 gtccgcccaa accagctcgc cggaacgcag gtccttgaga atcatcgaga acttgtagta
     1141 ctggtccacg gtgcggccga cctgattctt gatctcggtc agcgcgccgg tcagatacat
     1201 ctgcgccgcg ctctgctgac ccggcttgat cgctttcctt tgatcggtca gaccgaggtt
     1261 ggcctgggaa tccatgaact cgatatccgt gccggaggtc gatttgtcga caaagcggaa
     1321 gagccccgaa cgcaacagct gcatgcgcag cgaatcggtg atgctcacca tgtccacatg
     1381 ctgcgtggtg cggtttttga gcggctccac atccagcacc ggacgggtgt tccccactgc
     1441 ctcgcgcacg ccgggcgcgc tcagcaatga ctctgccatc ttctccactg tgcgccgcac
     1501 atcctgcggc tccagtttcg aagtgatcgg cgcacggtcg agcgccacct cgcgcaccgt
     1561 cggcgtggtc gcgcacccgg tcatcatcac ggccgccgct gcgacccctg ccatcccaat
     1621 ccaatttttg ttcatccgtt tgcctcgctt tcttttttcc gtaatcaatc cgccgcttcc
     1681 agatcgcgca cgcgcagttt cgacgtcacg cctgccgtcg agttggcgac accggtcacg
     1741 gtcaccgcgt cgcggccctc cagaatcaag gtgcggtacg tcttggtgtc cggatccagt
     1801 tccatgccgt tggcgtcgaa ccagatgatg cggtaatgca ggcgcagcct gcggtggcgg
     1861 gtcgaggcca gcgtgatgtg cacgcgcttc aacccgttca cctcgtcata gttgaccttc
     1921 attacctgca cctgacccct gagccgcgtg ctgtcctcca gcaccaccgt gctgccggtg
     1981 gtgtggtccg tggtcaccac cgtgccagcc gtctccacgc atccggtcag cgcggtcagg
     2041 ccggccgcca gcgcgaccgc tccgagcaac ccgtttatcc gtttcatcgt tctctccttt
     2101 gttgtccttc gtttcgtgat ccggggtttt cccggcctgt gaacactgcg ccgccttgtc
     2161 agggctgcct cgcccccagg gtccgcggcc cgcccggcaa cgtcaccggc gggaaacccg
     2221 cgagcaggct gccgcaaacc cggaacgtgg ccggcgcgcc cttcccgttt agcgatgccg
     2281 tcgccgcccg cgcgttaccg ccgaaatccg ccacccaaat cagacgcgtc tcaccctcag
     2341 ccaccttaac cggcacgcgt gtgacgagcc cgttcgcctg attccgcagt tcgatcacat
     2401 gctcgcccgg ctcgaccccg ccgcggtaga gatgcgacac caccggcagg gtgtaccaag
     2461 cccgcgtgtc ggctttgttg atcactgccg aaaccgcgtt aaaggccatc acgctgtatt
     2521 ttgccaagtc gcccgcctgg ttcgctccat attgcgcagc cacgcgggtg gccacacgcg
     2581 tcgcgttgcg caccacgatt ccgggaatct tttccttgag gtcgcggtac gccagcgatt
     2641 gcaccgacag cgccagcgcg atcattccca actcccgccc gccgcctcga atcgcgatcg
     2701 tcgacggagc atacagatcg tcgcggtaaa ccgggaaatc cacggaaacc acggtgttga
     2761 cccccggcac cggcaaaggc actttcatgg actggcgctg cgatataaac ccttcctcca
     2821 ggatcaggat gatttcggta gcgtctcgct tcaatgtggc agggtccgtg ccggtctgcg
     2881 ccaaaaggcc tttgtacgcc tcggcatcct gcgtgcgcaa cagccgcaag aaatctttct
     2941 gtatgtaagg gttgcccggc atcagctccc acgccttgcg gtatgagagc gacgcgttcg
     3001 cctgatcctg atcctgctcg aacagcaagc cgcaaagata ccaggtcagg ccgttctcga
     3061 aagaactgcg gctaagctcc agcaccggcg ccatcgactg catctgggcg tctactgttt
     3121 gactggcttt ggcgtgcttg ccgtcagcgg cttgggcttg aatctcctgc acctctttcc
     3181 cgtatttctc ggcgatctgc tcctgcgcga aaaccgcccg ccgcgcctcc accgccgcag
     3241 cgtccggcgc gcccagaaag atgtggttga gaagctggta ctgcaaggcg ttgatgaact
     3301 cgtaggccgg caaatgatac gaccgcgtgc ggtcgtccgt gatcgtgccc gccatcacgt
     3361 tagcccccac gctgcccatc ttgatcaccg gaccgctctc ggtatcctcg atgaccttgt
     3421 cgatcagaac cgagaagttg gtggaactgc cgtggaaatc accggacagc atccgcacac
     3481 gccccgtctc ggcataaccg agatccttgc tgtaaaaact cctcttgagt ttttccgacc
     3541 actccaacgc ctgcgggtcg tcaccggccc gcagactcgc caacgcccgg tccgtgctga
     3601 cattgggcga gacgcacccg gccgcaacga gcaacagcaa cgcgccgcat ccggcggaac
     3661 atgggattgt gttcctcatg aaccctccac tccatcagaa cagcgtgcgg cgcaacggga
     3721 accggctcgt cacgccgccc gctcgtcaat cactggatgg cgttctgctg caggttgatc
     3781 tcactgccct tgttccccac gtcacgccag gccgccgtgt caaagttgat cttcacccct
     3841 ttgcgcatca ccgcttcggc atagccgatg gcgaccgccg cgcggaaccc ctccagcgaa
     3901 cggtaccgct gcaagcccgc atccgacaac tcgagcgcca cccggaaagt cgcgccctgc
     3961 tggatgttca cggtctgctg ccacggccgc atccactgac gcgagacgcg caactggtgc
     4021 aggccgggcg tggccttgaa cgtgcccggc gaggaaccca cggtcgcacc gtccaactct
     4081 acggttaccc cgccgaccaa ccgccgcatc tcgtcgagca gttcgttcgg cgcgcgcaca
     4141 ccggactcca gaccctgaat caactcgtcc accgtggagg agacgctgaa ctcgaccagc
     4201 gcggtgtcga ccggcgcggc gcggcgccac gtggccacct tcccggcgat ctcctcaccc
     4261 gtggcggtcg accactggtc aaagagatcc tcataataat tcatcgcgtc cccgccgtcc
     4321 gtgtactgtc cgggcaccgg caacttattg gtccagttgg aaccgtaaac ggatga
//
LOCUS       DAOSRB010000228         4345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_18242_length_4345_cov_7.344988, whole genome shotgun sequence.
ACCESSION   DAOSRB010000228 DAOSRB010000000
VERSION     DAOSRB010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18242_length_4345_cov_7.344988"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLZ74_09925"
     CDS             <1..472
                     /locus_tag="PLZ74_09925"
                     /inference="COORDINATES: protein motif:HMM:NF021941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOR98680.1"
                     /translation="TAYVNARRELVTAGRALRADYNLAPSKFLHYVIQAMDEETAGRL
                     KADAETLKQQLRSDNLEVVAGGGEKAMPGTLCKLGTVYLPLEGVVDVKAEAARVKTEL
                     EKTRGFLKGVEAKLGNEGFVAKAPAAVVEQQRAKQKELTETIARLEKLLASFGG"
     gene            743..1843
                     /gene="ilvC"
                     /locus_tag="PLZ74_09930"
     CDS             743..1843
                     /gene="ilvC"
                     /locus_tag="PLZ74_09930"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOR98681.1"
                     /translation="MAKINFGGVVETVVTRKEYPLKKAQKVLKDEVIAVIGYGVQGPA
                     QSLNMRDNGIQVIVGQDKRFANDWKRAVKDGFKPGKTLFDIEEACARATIIMVLVNDA
                     AIRQVWPRIAPHLTAGKALYFSHGFGWVYRSLTRVKAGPDVDVIMVAPKGSGTSVRRN
                     FLAGVGINSSFAVAQDATGKALDRTLSIGIGVGSGYLFPTTFEHEVTSDLVGERGVLM
                     GALAGIMEAQYDVLRKNGHSPSEAFNETVEELTQSLIRLVDENGMDWMYSNCSATAQR
                     GALDWKPKFKKATLPVFKALYEAVKSQKEARHVIKVCGTADYKQKLDAELKVMRDSEM
                     WQAGAAVRALRPREKAKANAAKAVGVKGRGAN"
     gene            complement(1871..3034)
                     /locus_tag="PLZ74_09935"
     CDS             complement(1871..3034)
                     /locus_tag="PLZ74_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOR98682.1"
                     /translation="MNPVFYNAMVLREPYSGVEVTTHQLACALTRFGSLPLCICAPRG
                     HRPIPITSHTRMIRPYGTDRSRLLRILWEQTVLPVLLKRARAPLLHAPAYVAPRLAPC
                     PVVLTIHDLHVLTHPQFCRADNRLHYRLLLPPSIRRAAAVIVFSNYTRRVILDRFPEV
                     RDRLVLIPPGLTPNLARCTDHARLQSARQRYALPTSFLLFVGDLTQRKNILNLIRAFA
                     IVQGERPDLHLVLAGAADPATAAVIDNAIRQNAIRHRVSRIGYVSEEDLPVLYSLARA
                     FVFPSHDEGFGLPPLEAMACGCPVVCSGGAAVENCGDAAVNCDPRDRASIASAVSLLL
                     DHPAFRKEKIEAGYRKAAGFSWEKAARATDALYRSVLSAHGKHERPADEADGP"
     gene            complement(3054..3143)
                     /locus_tag="PLZ74_09940"
     tRNA            complement(3054..3143)
                     /locus_tag="PLZ74_09940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3107..3109),aa:Ser,seq:gga)
     gene            3387..4091
                     /locus_tag="PLZ74_09945"
     CDS             3387..4091
                     /locus_tag="PLZ74_09945"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOR98683.1"
                     /translation="MASVRGVRGGRCGTSRGEWMLRYESKVWDAAPGEVLAGVDEAGR
                     GCLAGPVVAGAVAMSAETAERLYAGEFAELTDSKQLTVARREAFFGRLAEMPEVTWAT
                     GLCNAEEIDRLNILSATHLAMRRAVEALAVRPTHVLVDGLPVKGLPCASTAIVQGDAK
                     SFLIAAASVVAKVTRDRMMCELDARFPQYGFAAHKGYGVNAHMAALFKYGPCAEHRHT
                     FRPVQDADQKLPGFEY"
BASE COUNT          841 a         1192 c         1537 g          775 t
ORIGIN      
        1 caccgcctac gtcaacgccc ggcgcgaact ggtcaccgcc ggacgcgcat tgcgcgccga
       61 ctacaacctc gcgccctcca agttcctgca ctacgtcatt caggcgatgg acgaagagac
      121 ggccggccgc ctgaaggccg acgccgaaac gctcaagcaa cagttgcgct cggacaatct
      181 ggaagtcgtc gcgggcggcg gcgagaaggc tatgcccggt acgctctgca agctcggcac
      241 cgtttatctg ccgctcgaag gggtggtgga cgtcaaagcc gaagccgcgc gtgtgaagac
      301 cgagttggag aaaacgcgcg ggttcctcaa aggcgtcgaa gccaagctcg gcaacgaggg
      361 gttcgtggcc aaggcgcctg cggccgtggt cgagcagcaa cgcgccaagc agaaagagct
      421 gacagagacg atcgctcggc tggaaaaact gctggcctcc tttgggggat gagaagatag
      481 acgaagacgc acgggcgtcc cgcctgtgaa cacgggcaag atgcccgtgc tggatggtcg
      541 actgaaaaag gcgaccttgt ttttcctgat ctgggggtcc aatcgccttt cggctgatcc
      601 cgtcgtcccg tcgtttaagc tgataaatgc gcttgcgcgt gcgccgcaag gtggtatgat
      661 tacgtttctt ttttcgcccg accttctggc catgtgcgcc gggttgttgg ggcggactga
      721 agttaaccga ggagtaaagg taatggccaa gatcaatttt ggcggtgttg tggaaaccgt
      781 cgtgacccgt aaagagtatc ccttgaagaa agcccagaag gtgctcaagg atgaagtcat
      841 cgccgtcatc ggctatggcg ttcaggggcc ggcccagtca ctgaatatgc gcgacaacgg
      901 gattcaggtt attgtcgggc aggacaagcg tttcgcgaac gattggaagc gggccgtcaa
      961 ggacgggttc aaaccgggca agaccctttt tgatattgaa gaggcctgcg cccgggccac
     1021 gatcatcatg gtcctcgtga atgatgcggc gattcggcag gtttggccgc gcatcgcgcc
     1081 gcatctgacc gctggcaagg cgctctattt ctcccacggg ttcggctggg tctaccggtc
     1141 gctcacccgc gtcaaagcag gtccggatgt ggacgtgatc atggtcgcgc ccaaggggtc
     1201 gggcacctcc gtgcgccgca atttcttggc tggcgtgggc atcaactcca gttttgcggt
     1261 ggcgcaggat gccaccggca aggcgttgga ccgcacgttg tcgatcggta tcggcgtggg
     1321 ttcgggttat ctgttcccca cgaccttcga gcatgaggtg acctcggatc tcgtcggcga
     1381 gcgcggtgtg ctgatgggtg ccctggccgg catcatggag gcgcagtatg atgtgctccg
     1441 caagaacggg cacagtcctt cggaggcgtt caatgagacg gtcgaggagc tgacccaaag
     1501 cctgatccgt ctggtcgatg agaacgggat ggattggatg tattcgaact gttcggccac
     1561 tgcccagcgc ggcgcgctgg actggaagcc gaagttcaag aaggccaccc tgccggtgtt
     1621 caaggcgttg tacgaggcgg tgaagagcca gaaggaagcg cgtcacgtga tcaaggtgtg
     1681 cggcaccgcg gattacaagc agaagctcga cgcggagctg aaggtgatgc gggattccga
     1741 gatgtggcag gctggcgcgg cggtgcgcgc gttgcggcca cgtgagaagg ccaaggccaa
     1801 cgcggcgaag gctgtcggcg tcaaagggcg cggagccaac taaggttcgc gcccgcggcg
     1861 cggaacggat tcaaggcccg tcggcttcgt cggcgggcct ttcgtgtttc ccgtgcgcgc
     1921 tcagcacgga tcggtacagc gcgtcggtgg cgcgtgcggc cttttcccag gaaaacccgg
     1981 cggctttccg gtagccggcc tcgatcttct ctttacggaa cgccggatga tccaacaaca
     2041 ggctgacggc actggcgatc gacgcgcggt cacgcgggtc gcaattgacc gccgcatccc
     2101 cgcagttctc gacggctgcg cctccggagc agacgaccgg gcacccgcag gccatggctt
     2161 cgagcggcgg caacccgaac ccttcgtcat gggaggggaa aacgaaggcc cgcgcgagcg
     2221 aatagagcac gggcagatcc tcttcgctca catacccgat tcggctgacg cgatggcgga
     2281 tggcgttttg ccggatcgcg ttgtcaatca cggctgcggt ggcggggtcg gccgcgcccg
     2341 ccaaaaccag atgcaggtcg ggccgttcgc cctgaacgat cgcaaaagcc cggatcaggt
     2401 tgaggatgtt tttgcgctgc gtgagatcgc ccacaaacag caggaacgat gtgggcagcg
     2461 cgtaacgctg gcgcgcggac tggagccgcg cgtggtcggt acaccgggcc aggtttggcg
     2521 tgagtccggg cggaatgagg acaagccggt cccggacttc cggaaaccgg tcgagaatga
     2581 cgcgccgcgt gtagttcgaa aaaacgatga ctgcggccgc ccggcggatc gagggcggaa
     2641 gcaggaggcg atagtggagc cggttgtcgg cacggcagaa ctgcgggtgt gtcaggacat
     2701 gcagatcatg aatggtgagc accactggac aaggcgcgag ccgcggcgcg acatacgccg
     2761 gcgcatgcag cagcggcgcg cgcgcgcgct tcagcaacac gggcaggacc gtctgctccc
     2821 agaggatgcg caacaggcgt gatcggtccg tgccgtatgg ccgaatcatg cgcgtgtgcg
     2881 aggtgatcgg gatcggacgg tgcccccggg gtgcgcaaat acagagcggc agcgaaccaa
     2941 agcgggtcag cgcgcaagcc agctgatggg tggtaacttc gacgccggaa tacggttcgc
     3001 gcagcaccat ggcgttatag aaaacgggat tcatggattc accgcaccgg gattggcgga
     3061 gagggaggga ttcgaaccct cggtacccgt aagagcacac agcatttcca gtgctgcacc
     3121 ttcggccgct cggtcacctc tccccgaaaa gttcccacgt tataccgggt tactgtgtgg
     3181 aaaacaatcc taaaatcctg gacgcttttt tcggcagatt ggcgcagatt tacgttttgc
     3241 cggttgccgc gccttgatat tttacattac cgatggcgat gcggtgtgtt accattcgtt
     3301 tcgtaagtgg aaacggttca ggtgcagagg ggtgcaccgg gaaaggggat acgggcacgg
     3361 atcacaggcg gaaccggttt tccgggatgg catctgtgcg cggcgtgaga ggcggccgct
     3421 gcggcacgag caggggggaa tggatgttgc ggtacgagtc aaaggtatgg gatgcggcac
     3481 ccggcgaggt gctggccggg gtggacgagg ccgggcgcgg ctgccttgcc gggccggtgg
     3541 tggcaggcgc ggtcgcgatg tcggcggaga cggccgagcg gttgtacgcg ggagagttcg
     3601 cggaactgac ggactcgaaa cagttgacgg tggcgcggcg cgaggcgttt ttcgggcggt
     3661 tagcggaaat gccggaggtg acgtgggcca cggggctatg caatgcggag gagatcgatc
     3721 ggctcaacat cctttcggcg acgcatctgg cgatgcggcg ggcggtggag gcgttggcgg
     3781 tacggccgac acatgtgctg gtggacgggc ttcctgtgaa ggggctgccg tgcgcctcga
     3841 cggcgatcgt gcagggcgac gcgaagagtt ttctgatcgc ggcggcgagc gtggtggcaa
     3901 aggtgacgcg tgaccggatg atgtgcgaac tggacgcgcg gttcccgcag tacggcttcg
     3961 cggcgcacaa ggggtatggc gtgaacgcgc acatggcggc gctgttcaag tacggtccgt
     4021 gcgcggagca ccgccacacg ttcagaccgg ttcaggacgc agaccagaaa ctgccggggt
     4081 ttgaatactg aacgggccgc gtttacgcac tgtcggcggt ccgagacgtc ccgccgtact
     4141 ttacctgcgg agttagaaaa ctggagctgg cgccggcttc ggggcgacac ggcaaagtac
     4201 ggtcggcgat tcagacgcgg tcggacacca cggttttttg tggatttacc ggacgggcgg
     4261 cggggtcggt gtgttcactc acgggataac cccaaaagga gcagttgaac gggtagaatt
     4321 ccagcaaagc cccgtaaaca ttggg
//
LOCUS       DAOSRB010000229         4317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_18405_length_4317_cov_6.850774, whole genome shotgun sequence.
ACCESSION   DAOSRB010000229 DAOSRB010000000
VERSION     DAOSRB010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18405_length_4317_cov_6.850774"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="PLZ74_09950"
     CDS             complement(<1..1542)
                     /locus_tag="PLZ74_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOR98684.1"
                     /translation="MTTQRKMSILASVLCAGVVQTGWTATSAYWTFQDQEPGVLATTL
                     VSEVHSDVLVGQGKSNGSGRLPSHDADVPATKIYDGLFGPLLNSNNTASLYFTNAVAY
                     PAQTNSQNGGYVEVADVPSLLCPTNFTIEFFVKIARDVDWPLLVGKARERGASWAIDI
                     DRNNSLKLRMDTQPIDQPGTTNGFNENLLGSNIQDGKWHHVAVTYSASKKTATFYVDY
                     EYVSSKASYAHEVTYNTNNYPLLMGRGAGSYQAFDGWLDEVRLSDVVLAPHQFLRTYA
                     VPEDAQLYYTFDDAATVPSTATTLVCEAHSPAMDGLTADTRPTFSPDIPPASTTRITQ
                     GYKGTVVNADNSSSLFFVNTGLPGNTDSAYGGQVAIPGFLHPSNFTVEVFFKMNRHVN
                     YPLLVGKVRTGSSPTWSLSLSGADDLVRCRFDTYPVDWNLGEHTYAENSQNGFNQCFS
                     TGVPGNDGKWHHAAFSYETATKNVKIYVDYVLKANGTTINPIVYTDGDILIGMGAGER
                     AFDGWI"
     gene            1996..3123
                     /locus_tag="PLZ74_09955"
     CDS             1996..3123
                     /locus_tag="PLZ74_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98685.1"
                     /translation="MRINVTTYLLAGLFVTAAGGAMADEACAPCETYRPKLFKRFGDH
                     VNTPDGLAQDRHGNIFLAAPNFVDKSYPGAILKMDKRSGKWSIFVTGLVHPETGHGAP
                     MGMEFGEDGNLYYCDNQYFSNKNYKSRVMRVVIDKAGEPVKIEPVVENIKLANALRVR
                     GNAIFFTDTFFDVAGKNLGGVYRVPISAFRDRPVRLLPKEQAANDPYCLGTTETKPLE
                     HRKDAAGADGLCIDKDGNLYTGNFGDGHFYILRLKPDGTYEKPATLFYDPKVFPCCDG
                     ICYYAKKNWIILTDSERNAVRYWDIKAGTMGLLWENEDTDGADGLLDQPCEPMVWGDK
                     LLVVNFDMSFPGLKNTRNDEVHTLSVIDLKEGGCPFLNLFR"
     gene            3270..3962
                     /gene="deoC"
                     /locus_tag="PLZ74_09960"
     CDS             3270..3962
                     /gene="deoC"
                     /locus_tag="PLZ74_09960"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOR98686.1"
                     /translation="MRVEPVTQTTMARMMDHAVLNPAMTENDIRRAAAMCRARGVGNL
                     CVRPTDAALAASLLKGSATTVAVVVGFPHGASRSEVKALEARLAIEDGAAELDMVMNI
                     GKFLSGDYEYVKRDIEAVVAVAKPQGVLVKVIQESCLLTLDQVAKACELTIAAGADFV
                     KTSTGFNGDGATVEQVEVMLKTCAGRTKVKPSGGIRDWERAAMFVRMGVDRLGVSSTD
                     KILDGAPTEDGC"
     gene            3994..>4317
                     /locus_tag="PLZ74_09965"
     CDS             3994..>4317
                     /locus_tag="PLZ74_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98687.1"
                     /translation="MNVQLSLVSGFQGFIQIGIGVGIAIGIDHLTTGHFLGFQGFTVS
                     RFHGFRVSRFHGFIQIGIGVGIAIGIDHLTTGHFLGFQGFWVSGFQGFIQIGIGVGIA
                     IGIDHL"
BASE COUNT          883 a         1221 c         1320 g          893 t
ORIGIN      
        1 aatccagccg tcaaaggccc gttcgcccgc gcccatgccg atcaggatgt cgccgtccgt
       61 gtaaacgatc gggttgatgg tggtgccgtt cgctttcagc acatagtcca cgtagatctt
      121 cacgttcttg gtggcggtct cgtaggagaa cgccgcatgg tgccacttgc catcgttacc
      181 ggggacaccc gtagagaaac actggttgaa gccgttctgg gaattttcgg cataggtgtg
      241 ctcacccaga ttccagtcta ccgggtatgt gtcgaaccgg cagcggacca aatcatccgc
      301 gccggacagg gagagcgacc aagtagggct tgaccctgtg cggactttgc cgacaagcag
      361 gggatagttc acatgccggt tcatcttgaa gaacacctcg accgtgaaat tggaggggtg
      421 cagaaagccg ggaatggcga cttggccacc gtaggcggag tctgtgttcc cgggcagccc
      481 cgtgttgacg aaaaacagcg aagagctgtt gtccgcattg accacggttc ccttgtatcc
      541 ctgcgtgatc cgggtggtgc tcgccggcgg aatgtcaggg ctgaaggtcg ggcgggtatc
      601 cgcggtaagc ccgtccatgg caggcgaatg ggcttcgcag acaagcgtgg tcgcggtgct
      661 gggcacggtt gccgcgtcgt cgaacgtata gtacagttgc gcgtcttccg gtaccgcgta
      721 cgtgcggagg aactggtggg gggccagcac gacatcgctc aggcgcacct cgtcgagcca
      781 gccgtcgaag gcctgataac tccccgcgcc ccttcccatc agaaggggat agttgttcgt
      841 gttatacgtg acttcgtggg cgtaactcgc cttggaggac acatactcat aatccacata
      901 aaacgtcgcg gtcttcttcg atgcgctgta ggtgacggcg acatgatgcc atttgccgtc
      961 ttggatattc gagccgagca ggttttcgtt gaacccgttg gtcgttcccg gctgatcgat
     1021 cggttgcgta tccatgcgca atttcagcga attgtttcga tctatgtcga tcgcccagct
     1081 tgcgccgcgc tcgcgtgcct tgccgaccag aagcggccag tccacgtcgc gggcgatttt
     1141 cacgaaaaac tcgatcgtga agttggtggg gcagagcagg gagggcacgt ccgcgacctc
     1201 gacgtaaccg ccgttctgcg aattggtctg ggccggatat gccaccgcgt tggtgaaata
     1261 gagcgaggcg gtgttgttgc tgttgagcag cgggccgaaa agtccgtcgt agatttttgt
     1321 cgccggcacg tccgcatcat gagagggaag ccggcctgag ccgttggact taccctgtcc
     1381 gacaagcacg tcgctgtgga cctcactgac cagcgtggtc gccaaaacgc ccggctcctg
     1441 atcctgaaag gtccaatacg ccgacgtggc ggtccatccg gtctgaacaa ctccggcaca
     1501 taacaccgac gcgagaatcg acatctttcg ttgagtcgtc atggttgttt ccttttttta
     1561 tagccacatc cgtgggacac acccgcatgt catgattacg atgaaaatat gcgataaagc
     1621 accggaaaaa tcaatcccga ttttgatctt tttctctatc ggggcgcatt cctgtttccc
     1681 gaaccgctga agggtttggt ttctgggttc agtcggatca gaggcctccc ccgccgcagg
     1741 tcaaatgccg caggccgcaa gtcgacatac acgcggtacc ggtaaaccca agaccaggaa
     1801 cccgaaacca gcacgggact gctgtttctg attccgcttt ccggtcatgc gcgttcctct
     1861 ggaaccccct atgccgtgac acgttttttg aggtgattgc cattcccgga gaggcttggt
     1921 aagatttcga tacgtttaat ccggatggca ggcggaggtc cgccgcagcc atcggcacaa
     1981 ggaaagggat atgcgatgag aatcaacgtg acaacgtatc tgctggcagg cttgttcgta
     2041 acggccgcag gcggcgccat ggcggatgag gcctgcgccc cgtgcgaaac gtaccggcca
     2101 aagctgttca aacggttcgg cgaccacgtc aacacaccgg acgggttggc tcaggaccgg
     2161 cacggcaaca tcttcttggc ggcgcccaat ttcgtggaca aatcctatcc cggcgcgatc
     2221 ctgaagatgg acaagcgttc cggcaagtgg tcgattttcg tgacgggtct cgtccatccc
     2281 gaaaccggac acggcgcgcc catgggcatg gagttcggtg aggacggcaa cctctactac
     2341 tgcgacaacc agtatttcag caacaagaac tacaaatcgc gcgtgatgcg cgtggtgatc
     2401 gacaaagcgg gcgagccggt caagatcgag ccggtggtcg aaaacatcaa actcgccaac
     2461 gccctgcgcg tgcgggggaa cgcgattttt ttcacggaca ccttctttga cgtggccggc
     2521 aaaaatctgg gcggtgtgta ccgcgtgccg atcagcgcgt tccgcgaccg tccggtccga
     2581 ctgctgccca aagagcaggc cgcgaacgac ccctactgcc tcggcaccac cgagaccaag
     2641 ccgctggagc accgcaagga cgctgctggc gcggatggtc tgtgcatcga taaggacggc
     2701 aacctctaca ccggcaactt cggcgacggc cacttctata tcctcaggct gaagccggac
     2761 ggcacgtacg agaagccggc cacgctgttt tacgacccga aggtgttccc gtgctgtgac
     2821 ggcatctgtt actacgcgaa gaagaactgg atcatcctga cggactccga gcgcaatgcg
     2881 gtacgctact gggacatcaa ggccgggaca atgggactgc tgtgggagaa cgaggacacc
     2941 gacggcgcgg acggtctgct ggatcagccg tgcgagccga tggtgtgggg cgacaagctg
     3001 ctggtcgtga acttcgacat gtccttcccg ggcctcaaaa acacccggaa cgacgaggtc
     3061 cacaccctta gcgtgatcga cctgaaagag ggcggctgcc ccttcctgaa cctgttcagg
     3121 tgatgtgttg cccgtgcggg aacgggaggg gtaagcgtct gcttgcccgt gaggacagtt
     3181 ccccgaaccg tccgcggcgg tttcggggaa actgccccat cttattttag ccgtttggcc
     3241 tccgggaaat gaggtccgag gagcccccca tgcgcgttga acccgtcacc caaacaacaa
     3301 tggccagaat gatggaccat gcggtgttga accccgccat gacggagaac gacatccgcc
     3361 gggccgccgc gatgtgcagg gcccggggcg tcggcaacct ctgcgtgcgc cccaccgatg
     3421 cggcgcttgc ggcatccctg ctcaaaggtt cagccacaac cgtcgcggtg gtggtcggtt
     3481 tcccgcacgg ggcgagccgt tccgaagtga aggcgctcga agcccggctg gccatcgagg
     3541 acggcgcggc cgagttggac atggtgatga acatcggcaa gttcctctcg ggtgactacg
     3601 agtacgtgaa acgggatatc gaagcggtcg tggcggtggc caaaccccaa ggcgtcctgg
     3661 taaaggtcat tcaagagtca tgcctgctga cccttgatca ggtcgcgaaa gcctgcgaac
     3721 tcaccatcgc cgccggagcc gatttcgtga agacctcgac cgggttcaac ggcgacggcg
     3781 caacggtcga gcaggtcgaa gtcatgctga agacctgcgc gggccgcacc aaagtcaaac
     3841 cctcgggcgg gatccgcgac tgggaacgcg ccgcgatgtt tgtccgcatg ggggtggatc
     3901 gcctgggcgt cagctcgacc gacaagattc tggatggcgc tccaacggaa gacgggtgct
     3961 aagataagac aaacgcggaa cctaaaagaa cagttgaacg ttcaactctc tttggtttca
     4021 gggtttcagg gtttcatcca aatcggtatc ggggtcggta tcgctatcgg aatcgatcat
     4081 ttaaccacgg gacactttct tgggtttcag ggtttcacgg tttcacggtt tcatggtttc
     4141 agggtttcac ggtttcatgg tttcatccaa atcggtatcg gggtcggtat cgctatcgga
     4201 atcgatcatt taaccacggg acactttctt gggtttcagg gtttctgggt ttctgggttt
     4261 cagggtttca tccaaatcgg tatcggggtc ggtatcgcta tcggaatcga tcattta
//
LOCUS       DAOSRB010000230         4304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_18479_length_4304_cov_6.779948, whole genome shotgun sequence.
ACCESSION   DAOSRB010000230 DAOSRB010000000
VERSION     DAOSRB010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18479_length_4304_cov_6.779948"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PLZ74_09970"
     CDS             complement(<1..1256)
                     /locus_tag="PLZ74_09970"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOR98688.1"
                     /translation="MRQFLCMLAGLMVVAGCAQKMWGQHGALAAPQALTVDYLQDPVG
                     LDHAAPRLSWKLIAARRSARDLRQVAYQILVASSPERLRDNQGDLWDSGRVVSDQSLN
                     VVYVGKPLVTSQRCYWKVRVWDNQSDAPTAWSATGRWIMGVMRPNDWQARWIGANAAT
                     RPDCDLAGAQWIWTGDAPSLEQAAQGVRYFRRVFDAPRGAQDAPVLLALTADDEYEVF
                     INGKRAAKTWGHFNESRWMRFQDVSALIKPGRNLIAATVKNKAPGPTALLAVLKFADG
                     QAVPTDTTWSSSAQAVDGWKEKADGFAHDAWKAAVIAGPVDGAPWGKIERRRELVSPA
                     FEKRFTVTRPVREATLHITGLGFYEASLNGQRIGRKVLDPVPTRYDRRVLYSTYDMTE
                     RLTRGENRLHVLLGHGWYDVRSVAVW"
     gene            1456..1596
                     /locus_tag="PLZ74_09975"
     CDS             1456..1596
                     /locus_tag="PLZ74_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98689.1"
                     /translation="MEGSRGFSGLLDNFRIYGRILNGQELRNVMLTDDSDVSRRLSIR
                     IH"
     gene            1608..2702
                     /gene="mnmA"
                     /locus_tag="PLZ74_09980"
     CDS             1608..2702
                     /gene="mnmA"
                     /locus_tag="PLZ74_09980"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOR98690.1"
                     /translation="MENSLPIVAVGLSGGVDSAVTAWLLQQQGHRVIGLTMSIWDPSF
                     GAATAKQSGCFGPGEAENLAAARAIAERLGIPHHTMHLAREYGCGVLDPFRAQHLEGR
                     TPNPCALCNPIMKFGLLPARAREKGIGFDRFATGHYARIVQDGETGLFRLLRGVDPTK
                     EQSYFLARLTQAQLARILFPIGAMAKSEVRETARRAGLGDLTEKPESQDFFEGEDIAA
                     LFHGAGTEPGPITDEAGRVLGQHRGIIRYTVGQRDGLGIAVGHKVYVKTIHADTNTLV
                     VAPREGVFSDRCRVNDFHWIAGEPPQPERALTAHLRYRHKGVSVQITFGPHPETFDLL
                     FAEPQFAVTPGQMAVLYDGDEVLGGGWIAV"
     gene            2840..3529
                     /gene="nth"
                     /locus_tag="PLZ74_09985"
     CDS             2840..3529
                     /gene="nth"
                     /locus_tag="PLZ74_09985"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOR98691.1"
                     /translation="MTCKDIPAVNRLLKTAFHSMRAPIVDLIAAQTRDPFRVLLGTIL
                     SARTRDACTAEACKRLFARVTTPDALRRIPQDELERLIFPVGFYRDKARHLKALPQVL
                     EQRFGGVLPDTVEELCELPGVGRKTANLVVALGFDKPAICVDVHVHRITNRLGLVNTK
                     TPFETEMALRACLPKRYWKTWNSYLVAFGQTRCLPRNPRCDGCPLATFCPSYAGKKRD
                     APGLPKASRQG"
     gene            complement(3540..3740)
                     /gene="rpsU"
                     /locus_tag="PLZ74_09990"
     CDS             complement(3540..3740)
                     /gene="rpsU"
                     /locus_tag="PLZ74_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOR98692.1"
                     /translation="MIQLRLKKGESVERALKRLKKIMDKEGLLKQLRNNRYYEKPCEK
                     ARRKSARARIRNNSRRAAREMF"
     gene            3932..4017
                     /locus_tag="PLZ74_09995"
     tRNA            3932..4017
                     /locus_tag="PLZ74_09995"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3966..3968,aa:Tyr,seq:gta)
BASE COUNT          818 a         1371 c         1262 g          853 t
ORIGIN      
        1 ttccagacgg cgacgctgcg cacgtcgtac cagccgtgtc ccagtagcac atgcaaccgg
       61 ttttcgccgc gcgtgagcct ctcggtcatg tcgtaggtgg agtagagcac gcgccggtcg
      121 tagcgggtgg gaacagggtc cagcaccttg cggccgatac gctgcccatt gaggctcgcc
      181 tcataaaagc cgaggccggt gatgtgcagc gtagcctcgc gcaccgggcg agtaaccgtg
      241 aaacgctttt caaaggcagg cgaaacgagt tccctgcgcc gttcgatctt accccaaggc
      301 gcaccgtcca cggggccggc tatcacagcg gctttccatg cgtcatgcgc gaaaccatcg
      361 gccttttctt tccagccgtc cacagcttgc gctgaggagc tccaagtggt gtcggtcggc
      421 acggcttggc cgtcggcgaa tttcagcacg gcgagcaggg cggtgggccc cggcgccttg
      481 tttttgacgg tggcggcgat caggttgcga ccgggcttga tcaaggccga aacatcctga
      541 aaacgcatcc agcgcgattc gttgaagtgg ccccaggttt tggcggcgcg cttgccgttg
      601 atgaacacct catattcgtc atccgccgtg agagccagca gcacgggggc gtcttgtgcg
      661 ccacgcgggg cgtcgaacac tctgcggaag tagcggacac cttgcgccgc ctgctccagt
      721 gagggggcat cgccggtcca aatccactgt gcgccggcca gatcgcagtc cggacgcgtg
      781 gcggcattcg cgccgatcca gcgggcctgc caatcgttcg gacgcatcac gcccatgatc
      841 cagcgtcccg ttgcactcca ggcggtgggc gcatcgcttt ggttgtccca gacgcgtacc
      901 ttccaataac agcgctgcga cgttacgagc ggctttccga cgtaaaccac attcaacgat
      961 tgatcggaaa ccacacggcc gctatcccag agatcgcctt gattgtcacg cagccgttcc
     1021 ggcgaagatg ccaccaagat ctggtaggcg acctggcgca ggtcacgcgc cgaccgacgc
     1081 gcggcgatca gtttccaact cagacgcggg gccgcatggt cgagtcccac agggtcttgc
     1141 agataatcga ccgtcagagc ctgcggggcg gcgagcgcac cgtgctgccc ccacattttt
     1201 tgtgcgcaac ctgcaacaac catcaagccc gccagcatac ataaaaactg tctcattcga
     1261 ctcccccaac ctaaattacc aacacgttga cactgtacac gaccgttcgt gcaggctcaa
     1321 tcaaaaagga aaccattcga atgacgcctc acgttgacac gtgaggttga acgaaacgga
     1381 aaacttccgc gcttcccctc catacgccgg atgtgcgata cttcgcacct gccgaatttc
     1441 tgttggaatt cgtcgatgga aggatcgcgc ggtttcagcg ggttactcga caacttccgc
     1501 atctacggcc ggatcctcaa cgggcaggag ttgcggaacg tgatgctgac ggatgattcg
     1561 gatgtctcgc ggaggctgag catccgcatc cactgaagga ggaccaagtg gaaaactctc
     1621 ttcccatcgt tgcggtgggg ctgagcggcg gcgtggattc agcggtcacg gcttggctgc
     1681 tccaacagca aggccaccgt gtgatcggcc tgaccatgag catctgggat ccgtcgttcg
     1741 gcgcggccac cgcgaaacag agcggctgtt tcggacccgg cgaagcggag aatctcgcgg
     1801 cggcccgcgc gatcgccgaa cgtctcggca tcccccacca caccatgcat ctggcccgcg
     1861 agtacgggtg cggggtcttg gacccgttcc gcgcacagca tctggagggc cgcacgccca
     1921 atccctgcgc cctctgcaat ccgatcatga aattcgggct cctgcccgca cgcgcccggg
     1981 agaagggaat cgggttcgac cgcttcgcca ccggccatta cgcacggatc gtccaagacg
     2041 gggaaacggg gttgttccgt ctgctacgcg gcgtggaccc caccaaagag cagtcctatt
     2101 ttctcgcgcg cctgacccag gcgcaactgg cccgaatcct ctttccgatc ggcgcgatgg
     2161 ccaagtctga ggtgcgcgag accgcccgac gcgcggggct gggtgatctg accgaaaaac
     2221 cggagagtca ggatttcttc gaaggtgagg acatcgccgc gcttttccac ggtgcgggaa
     2281 ccgagccggg accgatcacg gatgaggcgg gccgggtact gggccagcat cgcggcatca
     2341 tccgctacac tgtcggacaa cgggacggcc tcggcatcgc ggtcggtcac aaggtttacg
     2401 tgaagacgat tcatgctgac accaacacgc tggtggttgc cccgcgcgag ggggtctttt
     2461 ccgacaggtg ccgcgtcaac gacttccact ggatcgcggg cgaaccgccg caaccggagc
     2521 gcgcgctcac cgcgcaccta cgttaccggc acaaaggggt ttcggttcaa atcacgttcg
     2581 ggccccaccc cgaaacgttc gacctgcttt tcgcggaacc gcagttcgcc gtgacgccgg
     2641 gccagatggc cgtgctctac gacggtgatg aggtgctggg cggcggttgg atcgcagtgt
     2701 gatgatccgt tcgcaacagg agggaggaga cgtgccgtgg aggcggttgc cgtaatcggt
     2761 ttccgagccg gacttgacga acgcccgtca cctgtgagac aaaacgtgtg ttgggatcct
     2821 tcgttcagga ataagcctca tgacatgtaa agatattccc gccgttaacc ggctgctgaa
     2881 aaccgccttc cacagcatgc gcgccccgat tgtcgatctg atcgccgcgc agacccgcga
     2941 cccgttccgg gtactgctcg gcaccatcct gagcgcacgc acccgagacg cctgcaccgc
     3001 cgaggcctgc aagcgtcttt tcgcgcgggt caccacgccg gacgcgttgc ggcgcattcc
     3061 ccaagacgaa ctcgaacggc tgatctttcc cgtggggttc tatcgcgata aagcgcgcca
     3121 cctcaaagcc ctgccgcaag ttctggaaca acggtttggc ggcgtactgc ccgatacggt
     3181 cgaagaactc tgcgaactgc ccggcgtggg gcggaagacc gcgaatctgg tggtggcgct
     3241 gggtttcgac aagcccgcga tctgcgtgga cgtccacgtc caccgcatca ccaaccggct
     3301 cggtctcgtg aacacgaaaa cacccttcga gaccgagatg gcattgcgcg cctgccttcc
     3361 caaacggtac tggaaaacct ggaactccta ccttgtcgcg ttcgggcaga cacgctgcct
     3421 gccccgtaac ccgcgttgtg acggatgtcc gctagcgacg ttctgcccct cttacgccgg
     3481 aaaaaaaaga gacgcccccg gtcttccgaa ggcgtcacgt cagggctgaa cccattcgat
     3541 cagaacatct cgcgcgctgc gcggcgagag ttgttccgga tacgggcgcg tgccgatttg
     3601 cggcgggcct tttcgcacgg tttctcataa taacgattat tgcggagctg cttcaacaag
     3661 ccctccttgt ccataatctt cttcaaccgt ttgagcgcac gctcgacgga ctcgcctttt
     3721 ttcaatcgga gttggatcac gctgttcacc ccctttcaac ccgacctgcg gcgcggttcc
     3781 ggcgggtttc ccctccatga cacagccgct tggttaaaag aagtaaaata acagaatatg
     3841 ctgcctgccg tcaatccgtg accgggaaaa aagcatttgc cgcgttgaca tccgccccgc
     3901 tttttgcgat atttctggaa ctttttgttg cggtagggta ccgaagtggc caaacggggc
     3961 agactgtaaa tctgctggcg gacgccttcc ttggttcgaa tccaagccct accaccattt
     4021 tgaaaccttg gcgtttgccg aggtttttca ttttctcggg ctgaactcct cctttttgcc
     4081 gccccccatc cccccgcttc ccgtctcccg tccctttcta ccctctcatt gcgttcagcc
     4141 caataagcat tggcttttgt tgagaacccc gccgcacctg tttggcgcag gacgcggcgg
     4201 tgcgtccgac gctctttttg aactcttagt cccggcactt tgtcggttgc acttcgtcgc
     4261 aagcgcaatc gactgagttt tggagaccgg tgcgtatgcg ggac
//
LOCUS       DAOSRB010000231         4303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_18482_length_4303_cov_4.665725, whole genome shotgun sequence.
ACCESSION   DAOSRB010000231 DAOSRB010000000
VERSION     DAOSRB010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18482_length_4303_cov_4.665725"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..4108
                     /locus_tag="PLZ74_10000"
     CDS             146..4108
                     /locus_tag="PLZ74_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98693.1"
                     /translation="MKRFSWTFVAVALGMVLHAYDDAEIKVSAKIWDATDTNVWQRAV
                     PDFGPGSAVTFSGADSQYLYFGTNVTIGSLTVPNTVTPGSRAYYNIYPWRERWATSDD
                     PTWAGMKWGERGYTLTMDSGSEAPAVIGAFAPLAGNIYRLGCHVPIKMASDLKLVHNG
                     RTDWPGGSSGNGENVSLWMLGDISDDGGTHMLITEGPRYFTGKTWAHPVIALCGDNTF
                     SGDVDIRAWTRLLNKRGIHDETSGTQLGRDNCVYVTNDCGVLDLSGNTYGVDHHLYIG
                     GYTAFTSTVGKLTSMYGNPNATAVWKGPVTLVGDTAFGGAFSTYAILSMSSNMRIEGD
                     IDDDGNNRNLQLVCPNRITLAGKNTFGGNLYHNTGFLTIEHPEGFSGGGKLVLASDAV
                     YRMTAPNQPDLSMILYTNTVERHKIRLRLDGDFTYTPQSGFTNFTEFYKSGPGTLVWD
                     KTNKGIGGNSNMDFFCLDGDTIFDYTQNDTLRLSGHTYFATIGAGNGGRVVLKTKNNQ
                     KNICDFAGVDWSTGEIVKEGAATYKAATPIKNAPYLLNFIVRDGMIIGEKSRQPILGA
                     SASLPNTYGESLIWSNRTWVSAVDPDVANPVWCGFDAYTNDWTRGKCFIDVTPELLET
                     PIPPDFSVAALRFNTPPAGDTMTLTLSGTNDITGGCILITPEFGNDKTLRITGGAIGR
                     PTAGTMRIVSYATNTVVILESDLVQTNRANAVNFCVYHCKVQFTGDSDYSGDVGIVGG
                     ELEVWDNSFMGGLASAGNINRGIHLNQGARLTTHGDFTLRRTVGSYYVIPYFRVYEKG
                     GVLNVADGELATGESTGEIALYDNAAFTKTGKGHLNWNGGMARAGGTCMIGADKIHRY
                     TFTDGKVTMNGNGFTNFRESQNLITVMSNVVITGDCAFNSNLGGVNGGGNAVDCGAKT
                     AFYVGEGGVTFDVAGVARTVGNDGGDKAGFSGHPVGDFYVGSGDITFTNTSETAGSIV
                     FKELGLSRLTGRMIQCAPKQNSGDCTPPFLLPQAEYVVPADLDTYVYQMMDGYQDLWI
                     GRLTGEGAFRIYGRNNYMDPVAHIGRDADETFTFGGRLCAFSISENNNASARYMKIGT
                     NRWRLTNPAGEVRSSTRVANGDVLIACEPSDGEAGALGFCDIYLGDTKTPPNGVPRVL
                     TDGAYTITNHFRILASAPATACPALGGNAVTEASTFTGDIRLWHDIDLYSAGGTVTFT
                     GAFLNGDGNPHAIAKRGPGKVIIQHALPAFSAVNIAEGILELTAPFAIPEGATLDFDT
                     SVCTKENRHNIYTLLACPGGVSGRFEVIADIPFDWKVIYANDAIHLVWAKRGTLIQFH
                     "
BASE COUNT          958 a         1185 c         1249 g          911 t
ORIGIN      
        1 aacggacgtg gatccgcaga tgaaatcggt tgtaaaacca catctatggt agtattataa
       61 cctgcgtttt tgatattttg ttggttgttg gctaactatg aaatcgtgtg accatcggct
      121 tgatcactcg tgaaaaggag cggacatgaa gcggttcagt tggacattcg ttgcagttgc
      181 gttgggaatg gttttacatg cgtatgatga cgcagagatc aaggtgtcgg ccaagatttg
      241 ggatgcaacg gataccaatg tctggcagcg ggctgtgcct gatttcggac caggcagcgc
      301 tgtgacgttc tcgggtgcgg atagccaata tctctacttc ggcacgaatg taacgatcgg
      361 ctcattaacg gtgccaaaca ccgtgactcc gggatcgcgc gcgtattaca acatctatcc
      421 gtggcgtgaa cggtgggcaa cttctgacga tccgacgtgg gccggcatga aatggggtga
      481 gcgcggttac acgctcacaa tggattccgg ttcagaggca cctgccgtca tcggcgcttt
      541 cgcgccgctc gcggggaaca tctatcgact cggctgtcat gtgccgataa agatggcgag
      601 tgacctgaaa ctcgtgcaca acggccgtac agactggccg ggcggttcct cgggtaacgg
      661 cgagaacgtt tcgctctgga tgctgggtga tatttcggac gatggcggta cgcatatgct
      721 catcaccgag ggtccccgat attttacagg gaagacctgg gcgcatcccg tgatcgcact
      781 ctgcggtgac aatacgtttt cgggcgatgt cgacatccgc gcctggacgc gcctcctcaa
      841 caagcgcggc attcacgacg agacgagcgg cacgcaactc ggacgcgaca attgtgtcta
      901 cgtaacgaac gactgcggtg ttcttgacct ttccggcaac acatacgggg tggatcacca
      961 tctctatatc ggcggctata ccgcgttcac cagtacggtc ggcaagctca caagcatgta
     1021 cgggaatccg aacgcgactg ccgtttggaa gggccccgtc acactcgtcg gtgacacggc
     1081 gtttggcggt gcattcagta cgtacgcaat tttatcgatg tcttcgaaca tgcgtatcga
     1141 aggcgatatc gacgacgatg gaaacaaccg caatctgcag ctcgtctgtc cgaaccgtat
     1201 cacacttgcc ggtaaaaaca ccttcggtgg caatctctac cacaatacgg ggtttctgac
     1261 gattgaacat cccgaagggt tttccggtgg gggcaagctg gttctggcct cggacgctgt
     1321 ttaccgtatg acggcgccga accagcccga cctttcgatg atcctctaca cgaacacggt
     1381 ggaacgccat aaaatccggc ttcgccttga cggcgacttc acctacacgc cacagtccgg
     1441 cttcacaaac ttcacggagt tctacaagtc cggtcccggc acgctcgtat gggacaagac
     1501 gaacaagggg atcggtggca acagcaatat ggatttcttc tgtctggacg gcgacacgat
     1561 tttcgattac acgcaaaatg acacgctccg tctttccggg cacacgtatt ttgccacgat
     1621 cggcgcaggc aacggcggac gggtggtcct gaaaacaaag aacaaccaga aaaacatctg
     1681 cgacttcgcc ggtgtagact ggtccacggg tgaaatcgtg aaagaaggtg cggcgacgta
     1741 taaagccgcc accccgatca aaaacgcgcc ctacctgctc aacttcatcg tgcgggacgg
     1801 catgatcatc ggtgaaaaat cgcgtcagcc catcttgggc gcatcggcat ccctgccgaa
     1861 tacgtatggc gaatcgctca tctggagtaa ccgcacatgg gtgagtgcgg tcgatccgga
     1921 tgtcgcgaat ccggtttggt gcgggttcga tgcctatacg aacgattgga cgcgcggcaa
     1981 atgtttcatt gatgtaacgc cggaactcct tgaaacaccg atccctcccg atttcagcgt
     2041 ggcggcgctc cgtttcaaca caccgcccgc aggcgatacg atgacgctca cgctttccgg
     2101 cacgaacgac atcacgggcg gttgcatcct catcacgccc gagttcggaa acgacaaaac
     2161 gctccgcatc acgggcggcg cgatcgggcg ccccactgcc ggcacgatgc gcattgtgtc
     2221 gtacgcgaca aacacggtcg tcattctgga atcggacctc gtgcagacca accgagcgaa
     2281 cgcggtcaat ttctgcgtct accactgcaa ggtgcagttc acgggagaca gcgactactc
     2341 cggggacgtc ggtatcgtgg gcggcgaact tgaggtgtgg gataactcgt ttatgggagg
     2401 actggcttcg gccgggaaca tcaaccgagg cattcacctg aatcagggtg caaggttgac
     2461 gacacacggg gactttacgc tccgccgcac ggttggcagt tattatgtca ttccgtattt
     2521 ccgcgtctat gaaaagggcg gtgtcctcaa cgtcgccgac ggcgaactcg ccaccggcga
     2581 aagtaccggt gaaattgctc tctatgataa cgccgcgttc acgaaaaccg gcaagggaca
     2641 tctcaattgg aacggcggga tggcacgcgc aggcggtacg tgcatgatcg gtgcggacaa
     2701 gatccaccgg tatacgttta cggatggtaa ggtgacgatg aacgggaatg ggttcacgaa
     2761 cttcagagag tcccaaaacc tgattaccgt aatgagcaat gtcgtcataa cgggtgactg
     2821 cgcgttcaac tccaatctcg gcggcgtgaa cggcggtggc aacgcggttg actgcggcgc
     2881 gaaaacggcg ttctatgtgg gcgaaggggg tgttacgttc gatgtcgcgg gggtcgcgag
     2941 aacagtcggt aacgatggcg gtgataaggc cggtttctct ggccatccgg tcggggactt
     3001 ctatgtgggt tccggtgaca tcacgttcac caacacatcc gaaacagccg gaagcattgt
     3061 attcaaagaa ctcggcttgt cgcgtctcac aggcaggatg atccagtgcg caccgaaaca
     3121 gaacagtggc gattgcacgc cgccgttcct cctgccgcag gcggaatacg tggtgccggc
     3181 tgacttggac acgtatgttt accaaatgat ggacgggtat caggatctct ggatcggacg
     3241 cctcacgggc gagggcgcgt ttcgtatcta cgggcgaaat aattatatgg atccggtggc
     3301 tcacatcgga cgcgacgcag acgagacctt cactttcgga ggcaggctct gcgcattttc
     3361 aatctccgag aacaacaacg ccagtgcacg gtatatgaag atcggtacaa accggtggcg
     3421 tctcacgaat cccgcaggcg aagtgcgctc ttcgacgcgc gtcgcgaacg gcgacgtgtt
     3481 aatcgcgtgc gagccgtcgg acggagaagc cggcgcactc ggcttctgcg atatctatct
     3541 gggtgacacg aagacgccgc cgaacggcgt accgcgtgtt ctgactgacg gtgcatatac
     3601 gatcacgaac cacttccgta ttctcgcgag tgcccctgca acagcgtgtc cggccctcgg
     3661 cggaaatgcg gtgacggaag cttcgacttt cacaggggac atacgcctgt ggcatgatat
     3721 cgacctctat tccgccggtg gcacggtgac gttcacgggc gcatttctga acggcgacgg
     3781 caatccgcac gcgattgcga agaggggacc gggcaaagtg atcatccagc acgcgttacc
     3841 cgccttctcc gcagtgaaca tcgccgaagg catactcgaa ctcacagcgc cattcgcgat
     3901 cccggaaggg gcgacgctcg acttcgacac gtccgtctgc acaaaggaaa accgtcacaa
     3961 catctacacg cttttagcgt gccccggtgg tgtcagtgga cgcttcgagg tgatcgcaga
     4021 cattccgttc gactggaagg tgatctatgc caatgatgcg atccatctcg tttgggctaa
     4081 gcgcggcacc ctgatacagt tccattgagt ccttattgcg gagtgttgcg cctccgtgtc
     4141 gccgcaacgc ttcgcaattg gttcgccagc gctttgccaa cacacgggga ggcctgatat
     4201 tatcctccca tgataacctt catgtggcga ttgacatgaa atcatttgtt atcatccttc
     4261 tggtcgtggc aggtgtcctt gccacaaata tcgcatggcc tgc
//
LOCUS       DAOSRB010000232         4302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_18486_length_4302_cov_6.721686, whole genome shotgun sequence.
ACCESSION   DAOSRB010000232 DAOSRB010000000
VERSION     DAOSRB010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18486_length_4302_cov_6.721686"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            562..810
                     /locus_tag="PLZ74_10005"
                     /pseudo
     CDS             562..810
                     /locus_tag="PLZ74_10005"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
     gene            complement(849..925)
                     /locus_tag="PLZ74_10010"
     tRNA            complement(849..925)
                     /locus_tag="PLZ74_10010"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(889..891),aa:Ile,seq:cat)
     gene            986..1957
                     /locus_tag="PLZ74_10015"
     CDS             986..1957
                     /locus_tag="PLZ74_10015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOR98694.1"
                     /translation="MTREIASIIRANEAGRALQDFLADRFTYHTADEWMCRIAEGRVR
                     VNGHTATPDRILSVGDHLRYDASDIPEPPVDCTFRILIDDPLLLVIDKPGNLPCHPGG
                     RYFRHTLWALLQTQCGLAHPVFVNRIDRETSGLVLIAKTPEAGRNLARQFAGGRVTKT
                     YTVLVEGMFPERVEARGWLHPDPDSAVRKKRRFSPAPAGSAAPGDAAEWSETVFERER
                     VCDGFSVVTARPRTGRLHQIRATLLSLGFPVVGDKLYGVDEQCFLRFCSGGLTERDRA
                     RLRMGRQALHAVRLAFRHPHFGTPTDVDAPLPSDMEALIGGHAQACL"
     gene            complement(2095..4083)
                     /locus_tag="PLZ74_10020"
     CDS             complement(2095..4083)
                     /locus_tag="PLZ74_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98695.1"
                     /translation="MKSYVVSVVAVLAVLAVRAEDRTWTGAVDTDWNTAANWTDEAVP
                     GTSDNALLTNNAGTIQVWVTHSPETAVGSLTVSNVNASGHTTTLTITNAAFGVQAGSV
                     LFGVNSVLDIRDGGAVSYLGEASAAPFFNVRDGGEIRINNGGGLLLTNLYSATGGLNR
                     YMYVGDGSAGTIRIGDGGTFVMGASSAGVATNTLMRLGYNNGGNGLLEMTGDSTFVMP
                     EGNKDTSALYVGSGAGASGTVFLADNAKCLVSNVLWGGSANATGIVSLSGNALLRPFM
                     RARQYGFGNGANAYGELTLHDNAWFDGAGTADQHFYCGKDGGTGRLNVYGGTLQIRNF
                     FHIGFNGTGYFTLTNGLVDAMGQYGRGLTFGVANGNGKVVYANALISGGTLDIKTPPD
                     IYAVEAYSGLQVGKTVAKTVTTTSRVFSEMVQSGGVITNRAYLFIGQGMNATGTVVQT
                     GGSFINGTSDKDSERAFIGSFGGCGSYVLSNGTFSTLKDVYVGGFPDALWPPSLPNAE
                     STNHWNKYADFYTNKVSTGVLRIDGGSFAASNATIHVGAQGVGTLVIGKDAVCIARDI
                     VLDTNTSSTVRLEFGPDGIGTLCATNSLTVCAGAKLEVDATAYPAATTWTKLIDCTSR
                     TGAFAEADISVIGEGVVRQDRDEDIWLYRVRGTMISVF"
BASE COUNT          902 a         1366 c         1200 g          834 t
ORIGIN      
        1 acccgagcag tgccatcccg tgtgttcgtg gttaaataat cgatcccgat cccgaccccg
       61 accccgatga aaccatgaaa ccagaaaccc agaaacccag aaacccaaga gagttgaagg
      121 ttcaactgct ctttttagga taaattgaca ggatttcccg ctgcgcgggc gcatgtatcc
      181 aggattttca ggattgaacc caaaaggagc agttgaacgg gtagaattct agcaaagccc
      241 cgcaaacatt gggttttgac gtattgagat gcgggcggag cccgcaatct caaaggctgt
      301 aagactgtga ggcggtgaga ctgtgaggcg gttgtgttct gtggtgaaac aatcgctccc
      361 gatcccgatc ccgatagcga tcccgacccc gatgaaaccc tgaaaccatg aaacccgaaa
      421 ccctgaaaca atcgatcccg atcccgatag cgaccccgac cccgatgaaa ccatgaaacc
      481 atgaaccccg aaaccctgat accctgaaac cagaaacccc ggcatggatc gccgccgcgc
      541 agaagatcga ccagcacgta cgacgacgct tactatgccg acgagacgct acccccgatt
      601 agcttgacgc tccccaaggt ccaacaggaa catcaacgcg gacatctctt tccgtttttt
      661 gccaacatgt ttagcgaggg cgcgaaccgg aacgtgcaga gccgtcaccg gcaacggcca
      721 gggaaacaaa gacacgtccc ctttctccaa agacgcgggt caaccattgc gcgccggtgc
      781 ggcaaagtgg taacgtaccc ctgttcttga tccggatgaa tgccgtgatg ccagattgcg
      841 tcggtggatg gtgggcccag cagggctcga acctgcgacc aaaggattat gagtcctccg
      901 ctctgaccga ctgagctatg ggcccgtgac aaccgcgtca ctatatcacg ggttcacgat
      961 gcgcttcaac gccaaagggg tagagatgac acgcgagatc gcctccatca tccgcgcaaa
     1021 cgaagccgga cgcgcattgc aagactttct ggcggaccgg ttcacgtacc acaccgcgga
     1081 tgaatggatg tgccgcatcg cggaggggcg cgtgcgggtg aacggccaca ccgcaactcc
     1141 ggaccgaatc ctttccgttg gggatcatct gcgttacgac gcctccgata tccccgagcc
     1201 gccggtggat tgcacttttc ggattctcat cgacgatccg ttgctgctgg tgatcgacaa
     1261 acccggcaat ttgccgtgcc accccggcgg acgctatttc cggcacacgc tttgggctct
     1321 gcttcaaacg caatgcggcc tcgcgcatcc ggttttcgtg aatcggattg accgcgagac
     1381 ctccgggttg gtgctgatcg ccaaaacccc ggaggcgggc cggaaccttg cgcggcaatt
     1441 cgccgggggc cgggtcacga aaacctacac tgttctggtg gagggaatgt tcccggagcg
     1501 ggtagaggcc cggggctggc tgcatcctga tccggattct gcggttcgca agaaacggcg
     1561 tttctcgccg gccccggcag gttcagccgc gcccggtgat gcggccgaat ggtcggagac
     1621 cgtgtttgag cgtgaacgcg tgtgcgacgg gttcagcgtg gtgactgccc ggccccgcac
     1681 ggggcgtctc catcagatcc gcgcgacgct gctgtcactc ggtttccccg tggtcggcga
     1741 caaactgtac ggggtggacg agcaatgctt tctccgtttc tgctcgggcg ggctcacgga
     1801 aagggaccgg gcgcggctcc gcatggggcg tcaggcgctc cacgcggtcc gcctcgcatt
     1861 ccgtcacccg catttcggca cgccgaccga tgtggacgcc ccgctccctt cggacatgga
     1921 ggccctgatc ggcggccatg cgcaggcttg cctgtaactt gccaggatga caggattttc
     1981 aggattgaac agcatcatgt taatcatgcc ctaaacgtat tccagggttt ttcgcggcga
     2041 agccgcgcgg caggctaatt aactaattaa actgggttgc gggcttcgcc cgcattagaa
     2101 gacgctgatc attgtcccgc gcacccggta gagccagatg tcctcatcac gatcctgccg
     2161 caccacaccc tcgccgatca ccgagatgtc tgcctcggca aacgcacccg ttcgcgatgt
     2221 gcaatcaatc agctttgtcc atgtggtcgc ggccggataa gcggtggcgt ccacctccaa
     2281 cttggcaccc gcgcacacgg tcagcgagtt ggtcgcgcac agcgtaccga taccgtcagg
     2341 tccgaactcg agccggaccg tcgaactcgt gtttgtgtcc agcacgatat ccctcgcgat
     2401 gcagaccgcg tccttgccga tcaccagcgt cccgacaccc tgcgcaccca catggatcgt
     2461 cgcgttactg gctgcaaagc ttcccccgtc gatcctcaag accccggtgc tcaccttgtt
     2521 ggtgtagaag tccgcgtatt tgttccagtg gtttgtggat tcggcattgg ggagtgaggg
     2581 gggccacagc gcatccggga acccgcccac gtagacgtcc ttcagcgtgc tgaacgtacc
     2641 gttcgacagc acgtacgagc cgcatccgcc gaaggaaccg ataaaggcgc gctcactgtc
     2701 tttgtcgctc gtgccgttta taaacgaacc gccggtctgg acgaccgtgc cggtcgcgtt
     2761 cattccctga ccgataaaga gataggcgcg gtttgtgatc acgccgccgc tctgaaccat
     2821 ctcgctgaaa acacgcgagg tcgtggtcac ggtcttggcc acggtcttcc cgacctgcaa
     2881 gcctgagtag gcttcgaccg catagatgtc tggcggggtc ttgatgtcca gcgttccccc
     2941 cgagatcagc gcgttcgcgt acacgacttt cccgttgcca ttggccacac cgaaggtgag
     3001 gccccgccca tattggccca ttgcatcgac cagaccgttg gtgagggtga agtatcctgt
     3061 cccgttgaaa ccgatatgga aaaagttcct gatctgaagt gtgccgccgt aaacattcaa
     3121 tctcccggtc ccgccgtctt tcccgcaata gaagtgctgg tctgccgtcc ctgcgccgtc
     3181 aaaccaggcg ttgtcatgga gcgtcagttc gccgtacgca ttcgctccat tcccgaagcc
     3241 gtactgacgg gctctcatga atggccggag cagggcgttt ccggacagcg aaacgatccc
     3301 ggtcgcattt gccgaaccgc cccacagcac gttggagacc agacacttcg cgttatccgc
     3361 cagaaaaacg gtgcctgatg cgcccgcccc ggacccgacg taaagcgcgc tcgtatcttt
     3421 attgccctca ggcatcacaa acgtgctgtc gcccgtcatc tccagcaacc cgttcccgcc
     3481 attgttgtaa ccgagccgca tgagcgtgtt ggtggcgacc ccggccgatg acgcgcccat
     3541 cacgaacgtc ccgccgtcgc cgatccggat ggtccctgcg gaaccgtccc cgacatacat
     3601 gtaacggttc aatccgcccg tggcggaata gaggttggtg agcagcagac cgcctccgtt
     3661 gttgattcgg atctcacccc cgtcccggac attaaagaac ggcgcggccg aggcctcccc
     3721 gaggtaactc acggccccgc cgtcccggat atcgagcaca ctgttcacgc cgaacaacac
     3781 cgagccggcc tgcacgccga aagccgcgtt cgtgattgtc agggtggtgg tatggcctga
     3841 tgcgttgacg ttcgagaccg tcaaggaacc gaccgccgtc tcaggcgaat gggtcaccca
     3901 gacctgaatc gtccccgcgt tattggtcag cagcgcgtta tccgacgttc ccggcaccgc
     3961 ctcatccgtc cagtttgcag ccgtattcca gtccgtatca accgcccccg tccacgtccg
     4021 gtcctccgcc ctgaccgcca aaaccgccaa aaccgccact actgacacaa catacgattt
     4081 catagcccgt ttccttccgt tctgacaaac caacgcccta tatcacaacc attcatccgg
     4141 gtagaccgga aacaaaccgg ctcccatgct tgatatctac tatgtgggga ggggcgaaaa
     4201 gagtcaactc taaaaatccg atcgccagcg gcacaaaaag aagaagggtc gaggccgggg
     4261 gagaacaaga caggattaac aggatttaca ggattggaga tg
//
LOCUS       DAOSRB010000233         4276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_18627_length_4276_cov_8.088842, whole genome shotgun sequence.
ACCESSION   DAOSRB010000233 DAOSRB010000000
VERSION     DAOSRB010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18627_length_4276_cov_8.088842"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLZ74_10025"
     CDS             complement(<1..569)
                     /locus_tag="PLZ74_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOR98696.1"
                     /translation="MNKIIRKTQLSEEIFRMEVEAPLIARERKPGQFIILQVDDDFGE
                     RIPLTIADADPERGSITLVFQTVGKTTHLLAEKEEGDTIVALLGPLGQPSRIEKVGHV
                     VCVGGGIGVAPLYPIAQALKAIGNRLTIIIGARNKSLLIMEDEMRAIADDLIVVTDDG
                     SYGRKALVTEPLKELCEQTPKPDMAVAIGP"
     gene            complement(660..1499)
                     /locus_tag="PLZ74_10030"
     CDS             complement(660..1499)
                     /locus_tag="PLZ74_10030"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006307.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOR98697.1"
                     /translation="MTIAIIGLGLIGGSFARDLRKAGLGRTLLGVETSPEHAERALEL
                     GLVDRVVPLSEAVATAQVLLLATPVDAIERLLPQLLDAVESHQTVIDLGSTKFRIGEG
                     VREHPKRERYVAAHPMAGTENSGPDAALEDLFRGKTVLLCNLEWSAPDACKTALEIFH
                     AVGLKFEFMNAEQHDRHAAYVSHLSHAVAYALSLAVQSEEKAGYAVPRLAGGGFTSTV
                     RLAKSSPEMWVPIFEQNREAILKAVEAFSERMLTFKQCLEERDYVRLDALIREANQIR
                     DLL"
     gene            complement(1507..2409)
                     /locus_tag="PLZ74_10035"
     CDS             complement(1507..2409)
                     /locus_tag="PLZ74_10035"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR98698.1"
                     /translation="MPRLAPPPQGDYNTIDRMNIPCPVFDFHAHLYPDALARNVVEPL
                     ARRFGNPAAFDGTVDGMTRELAASGLCGALNLPVATKPGQVNSINTWAAANNRGPVYS
                     LATLHPDTPDIPDTLVALLAAGFRGVKLHPEYQTFRLDDLRLEPVWETCAALGLFVFL
                     HAGGERVFAPPFHSTPTDVAALLDRHPKLTLVAAHLGGFQMWDEAEERLIGRPLYLDL
                     SHTFFWMPDGQILRMVRRHGADRILFGSDAPWQKPAAVLAAFLNLPLREAEQRMILWE
                     NAARLLQLPVAERLAVPPPNPQTR"
     gene            complement(2430..4046)
                     /locus_tag="PLZ74_10040"
     CDS             complement(2430..4046)
                     /locus_tag="PLZ74_10040"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOR98699.1"
                     /translation="MSALLLLSAVCGVHAAPAFRADRNYPDLGLRFRVLANAQPEPLA
                     QAKTYSYTFTRGSESFQRDLFDPTELWLATQHAGQWRDADGNVLVLGRPTHLFPVIES
                     KTKHVSREMFDQAMDDPAVAFAPDNPEALTAWVARFADCTPRAPERLRTGFNLADALF
                     FPVSESSRLVYAFRVKTRGKDGAGLPSDWFCAVIRIGDSTPKAKVRQAFETQFLANVT
                     WLPQSSAAVTRDVRPQTLTPVPASGPPGTPAVIPEHPSREAVRKSIADMKDWWLAETP
                     EYLFLSDIRSSAGKVLVQELQKTLPALRAGFARLVPPFKAVDEVSVLRIYESREAYQQ
                     YVGKAYAWSTGLWSPMRRELVIQWQGRNRDKTLTIIRHEAFHQYLFYASAMIEHAAWF
                     NEGHACFFEMATVDAKNRVSIPENPRVDYLMRNLDKVTRHLPKLLQTGHKEFYQGSNE
                     QRDLNYTAAWALVYYLRKGAPAEKESAYAALPDAYLQALAQTRKAEAATAAAFEGVNM
                     DLFQRRFTEFWQRGRNRGQRYDPFGKLTAD"
BASE COUNT          795 a         1284 c         1388 g          809 t
ORIGIN      
        1 ggcccgatgg ccacggccat gtccggtttg ggcgtctgtt cgcacagctc tttcaacggc
       61 tcggtcacca gtgccttgcg gccgtaagag ccgtcgtccg tcacgacgat cagatcgtcg
      121 gcgatcgcgc gcatctcgtc ttccatgatc agcagggatt tgttgcgggc gccgatgatg
      181 atggtgaggc ggttgccgat ggccttcaag gcttgggcaa tcggatacag cggggccacg
      241 ccgatgccgc cgccgacgca gaccacatgc ccgaccttct cgatgcggga gggctggccg
      301 agcggtccca gtagcgcgac gattgtgtca ccctcctctt tttctgccag caggtgggtg
      361 gttttaccga ccgtctggaa aaccaatgtg atgctgccgc gttccggatc ggcgtcggcg
      421 atcgtgagcg gaatgcgctc gccgaaatca tcgtcaactt gcaggatgat gaactggccg
      481 ggcttgcgtt cgcgcgcgat cagcggtgcc tcgacctcca tgcggaagat ctcttcggag
      541 agctgtgttt ttcggatgat tttattcata atcgtctttc atcgaacagg aggaccacaa
      601 aacggggcga gcataacacg ttttttccgc agccgcaaga aagccgccga accatcgggt
      661 cagagcagat cgcggatctg gttagcctcg cggatcagcg cgtcgagccg tacgtagtcc
      721 cgctcctcaa ggcattgttt gaacgtcagc atgcgctcgg aaaaagcctc gacggctttg
      781 aggatggctt cgcggttctg ctcgaaaatc ggaacccaca tctccggcga acttttggcg
      841 agacgcactg tggaggtgaa tccgccgccc gcgagccgcg gtacggcgta tccggctttc
      901 tcctcactct gcaccgcaag ggacagggcg tacgctaccg cgtgggagag atgcgagaca
      961 taggcggcgt ggcggtcgtg ctgttccgca ttcatgaatt cgaatttcag ccccacggcg
     1021 tggaaaatct ccagcgcggt tttgcaggcg tcgggcgcgc tccactcgag gttgcagagc
     1081 aggacggtct tgccgcggaa cagatcttcg agcgcggcat ccggccccga gttctccgtg
     1141 ccggccatcg gatgcgccgc cacgtagcgt tcccgtttcg ggtgttcccg gacaccttca
     1201 ccgatgcgga acttggtgga tcctagatcg atgacggttt gatgggattc cacagcgtcc
     1261 agaagttgcg gcagcaaccg ctcgatggcg tcgaccggtg tggcgagcag cagcacttgt
     1321 gcggtcgcca ccgcttcgga aagcggcacc acccggtcga ccagtcccaa ttcgagcgcg
     1381 cgttccgcgt gttccgggga ggtttcaaca ccgagcaggg tccgaccgag accggcttta
     1441 cgcagatccc gggcgaaaga gccgccgatc aaaccgagac cgataattgc gatcgtcatg
     1501 ggtcgcctat cttgtttgag ggttgggcgg cggaacggcg aggcgttccg ctaccgggag
     1561 ttgcaggagc cgggccgcgt tctcccagag gatcatgcgc tgctcggctt cccgcaacgg
     1621 taggtttaga aaagcggcca gcaccgccgc aggtttttgc cagggcgcgt cgctgccgaa
     1681 gaggatccgg tccgccccat gccgccgtac catccgcagg atctgcccat ccggcatcca
     1741 gaaaaacgta tgcgaaagat ccagatagag cggtcgtccg atcaaccgct cttccgcctc
     1801 gtcccacatt tggaatccgc ccaggtgcgc ggccaccaga gtcaacttcg gatggcggtc
     1861 gagcaacgcg gccacgtccg tcggtgtgga atggaagggt ggcgcgaaga cccgttcgcc
     1921 gccggcgtgc aggaacacaa agagccccag cgcggcgcag gtttcccaga ccggttcgag
     1981 ccgcaggtca tccagccgga acgtctggta ttcgggatgc agtttgacgc cccgaaagcc
     2041 cgccgcgagc agggccacaa gtgtgtcagg gatatcgggc gtgtctggat ggagggtcgc
     2101 cagactgtag actgggccgc ggttgttggc ggcggcccag gtgttgatgg agttcacctg
     2161 gccgggcttg gtcgcaaccg gcagattcag cgcgccgcac aacccggagg ccgccagttc
     2221 gcgcgtcatg ccgtccacgg tcccgtcaaa ggccgcaggg ttaccgaaac gccgcgcgag
     2281 cggctccaca acgttacgcg ccaaggcgtc aggatagaga tgagcgtgga aatcgaaaac
     2341 tggacagggg atgttcattc ggtcgatggt attgtaatca ccctgcggcg gcggcgcaag
     2401 cctcggcact ccctcgacgg ggccgcggct cagtccgcgg ttagttttcc gaacggatca
     2461 tagcgttgac cgcggttgcg tccgcgttgc cagaattccg tgaaacggcg ctggaagagg
     2521 tccatgttga ccccctcgaa ggcggcggcg gtcgcagcct ccgccttccg ggtctgcgcg
     2581 agggcttgca gatacgcgtc aggcagggcc gcatacgcgc tttccttctc cgctggtgcg
     2641 cctttgcgca ggtagtacac aagtgcccaa gctgcggtgt agttcaggtc gcgctgctcg
     2701 ttcgatccct gataaaactc cttgtgaccg gtttggagca atttgggtag gtgacgcgtc
     2761 accttgtcca gattccgcat caggtagtcc acacgcggat tctctgggat ggagacccgg
     2821 ttcttggcgt cgaccgtcgc catttcgaag aaacaggcgt ggccttcgtt gaaccaggcg
     2881 gcatgctcga tcatggcgct ggcataaaac agatactggt gaaacgcctc gtgccggatg
     2941 atggtgaggg tcttgtcccg attccggcct tgccactgaa tcaccagttc gcgccgcatg
     3001 ggtgaccaga gtcccgtgct ccacgcatac gctttgccca catactgctg ataagcctcg
     3061 cgcgattcgt agatacgtag cacactcacc tcgtctacgg ccttgaaggg cggtaccagc
     3121 cgagcgaaac cggcgcgcag ggccgggagg gtcttctgca gttcttgcac cagcactttg
     3181 cccgccgaac tgcggatatc ggagaggaag aggtactccg gggtttcggc aagccaccag
     3241 tccttcatat cggcgatact tttacgcacg gcctcgcggc tcggatgctc tggaatgacc
     3301 gcaggggtgc ccggggggcc ggaggcgggg acaggcgtga gcgtttgggg ccggacgtcc
     3361 cttgtcacgg cggcgctgct ttgcgggagc cacgtcacgt tggctaggaa ctgggtctcg
     3421 aaggcctgcc gcactttggc tttcggtgtg gaatcgccga tgcggatgac cgcgcagaac
     3481 cagtctgaag gcaagcccgc gccgtccttg ccgcgtgtct taacgcgaaa ggcgtacacc
     3541 agacgcgacg attcgctgac cgggaagaag agggcgtcgg cgaggttgaa cccggtgcgc
     3601 agtcgctccg gggcacgcgg cgtgcagtcg gcaaagcgcg cgacccatgc ggtgagcgcc
     3661 tccggattat ccggggcgaa ggcaacggcg ggatcgtcca tggcctggtc gaacatctcg
     3721 cgggagacgt gttttgtctt tgactcgatc acggggaaca ggtgagtcgg ccgaccgagc
     3781 accagaacat tgccgtccgc atcgcgccac tggcccgcat gctgggtcgc gagccagagt
     3841 tccgtagggt cgaacaggtc acgctggaat gactcgctgc cgcgggtgaa ggtatacgaa
     3901 taggtcttgg cctgcgcgag cggttcgggc tgcgcattgg cgagcacacg gaaacgcaat
     3961 ccgagatccg ggtaattccg gtcggcacgg aacgcaggcg cggcatggac cccgcacacg
     4021 gccgacaaca acagcaacgc ggacacgtac cgcacattcc ggcacgtcgc gatcgcaacg
     4081 ggacttcgag aaatacgcat gcgggggagt atagcgcgtg cccggaaatc cggcaagacg
     4141 cggccggtgc agatactacc ccggccacaa tttatgccgc acgcgtggca tgaaaccaag
     4201 ccatgacttt ccgtgtgttc ttgtgtcccg tggtgaaaca atcgatcccg atagcgatcc
     4261 cgaccccgat ttggat
//
LOCUS       DAOSRB010000234         4217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_18967_length_4217_cov_7.653532, whole genome shotgun sequence.
ACCESSION   DAOSRB010000234 DAOSRB010000000
VERSION     DAOSRB010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18967_length_4217_cov_7.653532"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..579
                     /locus_tag="PLZ74_10045"
     CDS             52..579
                     /locus_tag="PLZ74_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOR98700.1"
                     /translation="MRHVLPDGNSGTAARKTFLFPALQAGAGLFHFFGHSNESSLGDG
                     DRFLSIANIQPGNWRIPPTSVVIACRANRWHGLTPTQLILPFGLFATDTGFAGCLGAT
                     GNMRAREGEELAVALFTPDTGEQTVTLGKVWLRGMRSMAGKIPQERLLCFSLIGDPAL
                     RFGVPPYPGTAVSVR"
     gene            complement(633..>4217)
                     /locus_tag="PLZ74_10050"
     CDS             complement(633..>4217)
                     /locus_tag="PLZ74_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR98701.1"
                     /translation="ADDAHNRLRAPTGPVRDAMEQAIAEARATDVATTAPAALLYRGL
                     MLSQEEKYAEAIPFLEEALRQDPSQQAGWEALGWAYIRTDQKERAWRLWEYFQQLMPD
                     EWMPYNLLAQGAVMQQDWRAADTHFKKALELRPDLFDLRFWYAQNLLRIGKPDEAEQI
                     FRQLIKEEPDRLDIQINLANLLTHKLEYDEAVELFRHINEELPGNAGFMMDQAVLELR
                     VGELKRADQLCLDVLELEPENPRAMALRADIAEIGNLGDSSVDRLQKVIDNTPDPVQR
                     GALRVRMANRCHMLNQRKPGLFSTRFILGQIRQAIDENPEDVAIRTLYAERLLQARRY
                     EECKPWAVEVLEKYNRHNLRAKTLLFELALVERRFEDAEQILHDRFSNFDPTDPMRYY
                     YEARLLVAQGAYREALQKLDQMEAAAEQGAVFTLLYHDLTESDWVPTTSVRRLHEHLA
                     ALQREGFVLISPTDIPAVIGLRPGERRQDSEPEEDAVPWTARLIDHVRYGITGERKFK
                     SGKKIRTHKSPMKVVAVTFDDALRAAFMLGAPVAEEFGVPFGMFAITRPNEEYVPSVA
                     GWNEMREYAASGNWIIGSHLYGAHYEQPVDKEGKFMRASLPNRVWLPEKNRLESMNEW
                     DRRMRREFRLSNKILHDEMGEEAHPVGMVAYPFGDIGQESACNLSVLKNPTQSIVSEA
                     ARHYQVGFVQGISGYTVAGDNLMLVRRYEPDWTDEGADVVRHAYEYHPVFMARRTRVE
                     IAFLMNKPHLAEEMLDLLKRDGYPEELCRKIIVETRSHFRNRPQRDVQPLVSSSMVAV
                     ESTAGGGQTSPTHGAQTMQGAAARDPDAPIDPTAREAVAGRESDLTEQEGVGHYQEGN
                     PDPWVYLSHPFIGGEVAHSKANDQFEILRYGGRAGLNLNRNLQLSAEYFESRIEQRIR
                     PRWNAVAYDPDDMTRFLFKALKREARARLSYRTASGAVLSGSLGVANLDPDYDEDALF
                     NLDMEDYPDSGEFDPADDDNEVIGDLSAHWAPRDDLTLFVYYARDLVTSAVKSLTSDS
                     VGAVARWKPKDTWHINLRSQYWSYEDDNALFYIQGESFWELMPDVGVWGGLDFSTVTS
                     SDASDYYWTPYWDQRLLGVLRYLQAWQGYTFRLDLLGGLQREDGRPLRRSDDLGLSGK
                     SDWELVWGASGTYNKRLGSYLDLFIDGSVMALRDYIDHRFLIGFNLGF"
BASE COUNT          808 a         1320 c         1266 g          823 t
ORIGIN      
        1 aacggtatcg atcggctgca agaacctctg aggcgcgccg ggcgatcagt catgagacac
       61 gttctgcccg acggcaactc cggaacggcg gctcgcaaaa ccttcctctt tcctgcgctg
      121 caggccggtg ccggactttt tcactttttc gggcactcca acgaatctag tctgggtgac
      181 ggtgaccgat ttctgtccat tgccaacatt cagcccggta attggcggat cccgccaacc
      241 agcgtggtga tcgcatgccg ggccaaccgc tggcacgggc tgactccgac ccaattgatc
      301 ctgccgtttg gccttttcgc aacggacacg gggttcgccg ggtgcctagg cgccaccggg
      361 aatatgcggg ctagagaagg cgaggaactc gcggttgcgc tgttcacgcc cgataccggg
      421 gaacagacgg taacgctggg caaagtgtgg ttgcgcggca tgcggagcat ggcgggaaaa
      481 atcccgcagg aacgcctgct gtgtttcagc ctgatcggcg atccggcgct ccgctttggc
      541 gtcccgccct atccgggaac cgcggtcagt gtccggtaac ggatcttcat ctgcggaagc
      601 agtggttgac aacctttcgc aaaagcggag aactagaacc ctagattgaa gccgatcagg
      661 aagcggtggt cgatgtagtc gcgcagggcc atgacgctgc cgtcgatgaa aagatcgagg
      721 tagctgccca accgtttgtt gtacgtaccc gacgcgcccc aaaccagctc ccagtcgctc
      781 ttgccgctca agcccagatc atcagagcgc ctcagcgggc gtccgtcttc acgctgcaag
      841 ccgccgagca gatcaaggcg gaaggtgtat ccctgccacg cttgcagata gcgcaacaca
      901 cccagcaggc gttgatccca gtacggagtc cagtaatagt cgctggcgtc gctggaggtc
      961 acggtcgaaa agtcgaggcc gccccagacg ccgacgtcgg gcatgagttc ccagaacgac
     1021 tcgccctgaa tatagaagag cgcgttgtcg tcctcgtaac tccagtactg gctgcgcaga
     1081 ttgatgtgcc aggtgtcttt cggcttccag cgcgcgacag cgccgacgga atccgaggtc
     1141 aacgacttca ccgccgaggt caccagatcg cgcgcatagt agacaaacag cgtcaggtcg
     1201 tcgcgcggag cccagtgcgc ggaaagatcg ccgatcacct cgttatcgtc atcggccgga
     1261 tcgaactcgc cgctgtccgg gtagtcttcc atgtcgagat tgaagagagc gtcttcgtca
     1321 tagtccggat cgaggttcgc gacaccgagc gacccggaga ggaccgcgcc gctggcggta
     1381 cggtaagaga gccgtgcacg cgcctcgcgc ttgagtgcct tgaagaggaa acgggtcatg
     1441 tcatcgggat catacgcgac ggcattccag cgcggacgga tgcgttgctc aatgcggctc
     1501 tcaaaatact ctgcggagag ttgcaggttg cggttgaggt tgagcccggc gcgcccgccg
     1561 tagcgcagga tctcgaactg gtcgttggcc ttgctgtgcg ccacttcgcc accgatgaac
     1621 ggatgcgaga gataaaccca aggatcggga ttgccctcct ggtagtggcc cacgccctct
     1681 tgctccgtca gatccgactc ccgtccggcg acggcctcgc gggcggtggg atcaatcggc
     1741 gcatccggat cacgcgcggc ggcaccctgc atcgtttgcg cgccgtgggt cggcgaagtc
     1801 tggccaccgc cagcggtact ctcgaccgcc accatcgagg acgacacgag cggctggacg
     1861 tcgcgctgcg ggcggttacg gaagtgcgac cgggtctcca cgataatctt ccggcacagc
     1921 tcttcggggt agccgtcgcg cttgagcaga tccagcatct cttcggccag atgcggcttg
     1981 ttcatgagga aagcgatctc gacccgtgtg cggcgcgcca tgaagaccgg gtgatattca
     2041 taggcatggc gcacaacgtc cgccccctcg tcggtccagt ccggttcgta ccggcggacc
     2101 aacatgagat tgtcgccggc cacggtatag ccgctgatgc cctggacaaa accgacctga
     2161 tagtgacgcg ccgcctcgga aacaatgctc tgcgttgggt ttttgagcac agacaggttg
     2221 caggcactct cttggccgat gtcgccgaac ggataggcca ccattccgac cggatgtgct
     2281 tcctcgccca tctcgtcgtg caaaatttta ttgctcagcc ggaactcgcg gcgcatgcgc
     2341 cggtcccact cgttcatgga ttccaagcgg ttcttttccg gcagccagac ccgattgggc
     2401 aggctcgccc gcatgaattt gccctccttg tcgaccggtt gctcataatg tgcgccataa
     2461 aggtggctgc cgatgatcca gttgccggaa gcggcatatt cgcgcatctc attccaaccg
     2521 gcgacagagg gcacgtactc ctcgttcggc cgggtgatcg caaacatacc gaacggcacg
     2581 ccgaactcct cggcgacggg tgcaccgagc ataaacgcgg cgcgcagggc atcgtcaaaa
     2641 gttaccgcca cgaccttcat cgggctcttg tgcgtacgga tctttttgcc tgacttgaat
     2701 tttctttcgc ccgtgatgcc gtagcgcaca tgatcgatca ggcgcgcggt ccaaggcacc
     2761 gcgtcctctt cgggttccga atcttgacgc ctctcacccg gccgcagccc gatgacggca
     2821 gggatatccg tcggggaaat taaaacgaac ccctcgcgtt gcagcgcggc gaggtgttca
     2881 tgcagccgcc gcaccgatgt ggtcggcacc cagtcgcttt cggtcagatc atgatagagc
     2941 agtgtgaaga cagccccctg ctccgccgca gcctccattt ggtcgagctt ctgaagcgct
     3001 tcgcggtacg cgccttgcgc gacgagcagg cgcgcctcat agtaatagcg catcgggtct
     3061 gtcgggtcga aattcgaaaa ccggtcgtgc aaaatctgct cggcatcctc gaaacgccgc
     3121 tcgaccaaag ccagttcaaa aagcagcgtc ttggcgcgga ggttgtgccg gttgtacttt
     3181 tcaagcacct cgacggccca gggcttgcac tcctcgtagc ggcgggcctg caaaagacgt
     3241 tccgcgtaca gcgtccggat ggcgacatcc tcggggtttt catcgatcgc ctgccggatc
     3301 tgacccaaga taaagcgtgt cgagaacaag ccgggtttgc gctggttgag catatggcaa
     3361 cggttggcca tccgcacacg caacgcgccg cgctgcacgg gatccggcgt gttgtcgatg
     3421 accttctgca ggcggtcgac actactgtcg ccgaggttcc cgatctcggc gatatccgcg
     3481 cgcaacgcca tggcgcgcgg gttttccggc tccagttcca gcacatccag acacaactgg
     3541 tcggcacgct tcagctcgcc cacccgtagc tccaaaaccg cctgatccat cataaacccg
     3601 gcattgccgg gcaactcttc gttgatgtgg cggaaaagct ccacagcctc gtcatattcg
     3661 agtttgtgcg tgagcaggtt ggcgaggtta atctggatgt ccaggcgatc cggctcttcc
     3721 ttgatcaact gccggaaaat ctgctcggcc tcatcaggct tgccgatacg tagcaggttc
     3781 tgcgcatacc agaaacgcaa gtcgaaaaga tcgggccgca gctcgagcgc ttttttgaaa
     3841 tgggtgtcgg cggcacgcca gtcctgctgc atgaccgcac cctgcgccag caggttgtag
     3901 ggcatccatt catccggcat gagctgctgg aaatactccc acagccgcca cgcgcgctct
     3961 ttctggtcgg tgcggatata cgcccaaccc aaagcctccc agccggcctg ctgggaagga
     4021 tcctgccgca gcgcctcttc caaaaaaggg atggcctcgg cgtacttctc ttcctgcgaa
     4081 agcatcagac cgcgatacag cagggcggcg ggagctgtcg tggccacgtc cgtcgcgcgc
     4141 gcttcggcga tcgcctgctc catggcgtcg cgaacaggcc ccgttggagc gcgaagccgg
     4201 ttatgtgcat catctgc
//
LOCUS       DAOSRB010000235         4196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_19079_length_4196_cov_8.513161, whole genome shotgun sequence.
ACCESSION   DAOSRB010000235 DAOSRB010000000
VERSION     DAOSRB010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19079_length_4196_cov_8.513161"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            434..1429
                     /locus_tag="PLZ74_10055"
     CDS             434..1429
                     /locus_tag="PLZ74_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR98702.1"
                     /translation="MRILLTASVLCCVSALSAADACCAAANPYVGRWALTIPGGRAGW
                     LGVEQLTCGALKGSILWGGGSVVPVAETRMENGALLLTRKHNRKKEGKEVQHIETITA
                     RREGNALALLTRTADENGKELSRAEFTGVKIPDLPSRPDLSKLRFGTPVALLARNDLS
                     GWKVMNEKAPNCWSVKDGVLSNRVAGPDGKNRHGTNIRTEAEFEDFNLKTEVRVPKGG
                     NSGIYLRGIYEVQVAETYGRPVNPHNMGALYSRITPAIAAEKPAGEWQTFDITLVDRH
                     LTVILNGKTIIDNQPVLGCTGGAMTSDEFRPGPIYLQGDHTDVDYRNMVITPIVK"
     gene            1443..2381
                     /locus_tag="PLZ74_10060"
     CDS             1443..2381
                     /locus_tag="PLZ74_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOR98703.1"
                     /translation="MNAARNLLPWFRLFRLPNLLTVPGDPVAGFLLAAGTGLTRNHLL
                     PLLAAAGASLGLYVFGLILNDLLDVATDARERPDRPLPAGEITVPQARMAAIAAALSG
                     LNLAATAGRAALLTAAALAGLIVLYNAVLKRIPVVGVLTMGLCRGLSLMVGAVTACPG
                     LWTQPDVSRLPVLLAAAGLSLYVAAFSVLALREAEPDAVRHPSVRWLPLITLSFTLPT
                     LFAMMAAQGRIGSGIGPAFGVFLMTLALLQAWLTGGPLHSVLPVPELVGRHICNLLRV
                     QACFCAATGIGGILPAVCLTALSFAFVPLAKRFYSS"
     gene            complement(2541..2906)
                     /locus_tag="PLZ74_10065"
                     /pseudo
     CDS             complement(2541..2906)
                     /locus_tag="PLZ74_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
     gene            complement(2957..>4196)
                     /locus_tag="PLZ74_10070"
     CDS             complement(2957..>4196)
                     /locus_tag="PLZ74_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOR98704.1"
                     /translation="RSSRVECRGSRVDAPDPDTDSDPDTDIDFDVDRVHPDAACSHIM
                     DDPNTYLALFFEPVRPFLQDDAVSEILINGPNQVYIELKGRLQATEARFVSEPALKAA
                     AVNVAKNVGRLLNEENPRLDARLPDGSRVHAVIPPLSRCGTVVAIRKFKKEKLTMEQL
                     IAFGSITPAGAQLIDAIVKLHKNVIVSGATSSGKTSVLNVLSGFIPENERILVIEDAS
                     ELQLQQTHTVCFETRKPDKNDKGEVTIRDLIHSALRLRPDRLVIGEIRGGEALDLLQA
                     LNTGHAGSMSTIHANSSRDSLSRIETCSLLSGIDIPLSALRSQVASAIDVVVHTARLS
                     DGSRKITAISEVLGLKDGEYVLQDLYRFDVRGIGPDGSLDGVFTGTGQKPTFLHEAAL
                     RKLPLDEAMFEKEGNTNAGR"
BASE COUNT          757 a         1334 c         1270 g          835 t
ORIGIN      
        1 ggatcgggag cgtcgacccg cgacccgcga cattcgaccc gcgacgatcg acaggagccg
       61 tccgcgtttc cgacaccgca ggtcgctcgt cgcgggtcgc gggtcgacat aaggtctgac
      121 gggagaagcg ggcgtcccta ccggttggcg gacgccttag tcccgcatac gcaccggtct
      181 ccaaaactca gtcgattgcg cttgcgatga agtggagcga tgaagtgccg ggactgagag
      241 ttcaaaaaga gcgtcgggcg caccgccgcg tccctttttc tgaaggtgcc gggggtgagg
      301 cgaaacctgc ccggatgaga ggaagtttag gaagagaggt gcggaatgtc aatggaaccg
      361 cgattcgtac ttgcgttccc ggaatgaaaa cgggaaacta tcacttatga aaacaaacct
      421 ttctgtttca cggatgcgta ttcttctgac cgcaagtgtc ctgtgctgcg tttcggctct
      481 ttccgctgcc gacgcctgtt gtgccgccgc gaacccgtat gtcggacgct gggctctgac
      541 gattccgggg ggccgtgccg gttggctggg cgttgagcaa ctcacatgcg gcgcgctgaa
      601 aggctcgatc ctctggggcg gcggcagtgt ggtcccggtt gccgaaaccc gtatggagaa
      661 cggcgcgttg ctcctcaccc gcaaacacaa ccggaaaaaa gagggtaaag aggtccagca
      721 tatcgaaacg atcaccgccc ggcgcgaggg caacgcgttg gcgctgctga cccgcacggc
      781 ggatgagaac ggcaaggaac tctcccgcgc ggaattcacc ggcgtcaaga tccccgacct
      841 gccttcccgc ccggacctgt ccaagctccg tttcggcacg cctgtcgcgc ttctcgcgcg
      901 aaatgatctc tcgggatgga aagtcatgaa cgagaaggcc cccaactgct ggagcgtgaa
      961 ggacggcgtc ctgagcaacc gggtcgcggg tccggacggg aagaaccgtc acggcaccaa
     1021 catccgcacg gaagcggagt ttgaggattt caacctcaag accgaggtcc gcgtgcccaa
     1081 gggcggcaac agcggcatct atctgcgggg tatttatgag gtgcaggtgg ccgaaaccta
     1141 tggccggcct gtgaatccgc acaacatggg cgcgctctac agccgtatca cgcccgccat
     1201 cgcagcggaa aaaccggctg gcgaatggca gacgttcgat atcacgctcg ttgaccgcca
     1261 tctgaccgtg attctgaacg ggaagaccat catcgacaac cagccggttc tcgggtgcac
     1321 cggaggcgcc atgacctcgg atgagttccg gcccggcccg atctatctgc aaggcgacca
     1381 cacggatgtc gattaccgga acatggtgat cactccgatc gtgaagtgag gcgtcgcgcc
     1441 ggatgaacgc ggcccgaaat ttgttgccgt ggttccggct gttccggttg cccaacctgc
     1501 ttaccgtccc cggtgacccg gtggccggtt ttctgctggc tgccggaacg ggtttgacgc
     1561 ggaaccacct gctgccgcta ctcgccgctg cgggcgcatc gctcggcctc tacgtgttcg
     1621 gcctgatcct caacgatctg ctcgacgtcg caaccgacgc gcgcgaacgt ccggatcgcc
     1681 ccttgcctgc gggggagatc acagtgccgc aggcgcggat ggcagccatc gctgccgcgc
     1741 tctccggtct caacctcgcg gcaacggcgg gacgtgccgc attgctgact gccgccgcac
     1801 tggccggcct gatcgtactc tacaacgctg tgttgaaacg tattcccgtg gtcggcgtcc
     1861 tcaccatggg gctctgccgg ggcctgagcc tcatggtggg cgcggtcacg gcctgtcccg
     1921 gcttgtggac gcaacccgac gtttcccgtc tgcccgtact tctggctgcg gcaggactgt
     1981 ctctctatgt ggcggccttc tctgtcttgg ccctgcgcga ggcggagccg gatgccgttc
     2041 gccacccttc cgtccgctgg ctgcctctga tcacgctgtc ctttacgttg ccgaccctgt
     2101 tcgcgatgat ggctgcgcag ggccgcatcg gaagcggcat cggcccggct ttcggtgttt
     2161 ttctgatgac ccttgccctg cttcaagcct ggctgacagg cgggccgttg cattcggtct
     2221 tgccggtgcc cgaacttgtg ggacggcaca tctgcaacct gttgcgggta caggcctgtt
     2281 tctgtgccgc gaccgggatc ggcggcatac tgccagccgt atgccttacg gcgctatcgt
     2341 tcgcgtttgt tccgctggcg aagcggtttt acagtagttg aaccgccccg cgtgactggc
     2401 cgcgccggac aataacccga gcggccttct tcctgcggtt cggccaccat gccgtacagc
     2461 gcgtgacgtt cctccagccc gtaatccggc acgcggaacg tcggttccag aaaatccgcc
     2521 agcaccttac ggagccgcac aggatcatgg gtctcgcact cccacaccac cagggtccgc
     2581 caaccgtcac gccacaactg ccgcaacgcc tcccggtcac gccgcacgtt gttggcgaac
     2641 ttctttttcc agaactgccg gtgcgtggcg ggtatccgcg ccccgatccg gcacccgtgc
     2701 gcatgccaga aacagccgtg gacgaaaatc actttccggt gacgcggcag aaccagatcg
     2761 ggtttccccg gaagtcgttt gtcatgcagc cggtaacgga accccatggc gaaaaccgcg
     2821 cgccgtactg cccattccgg cgcggtatcc ttgctgcgga tgcgcgacat gcagtgatgc
     2881 cgctgctctg gggtcatgcg atccattttt ttataacgct gaagggtgac tccgttttcc
     2941 gtctggcgtt ggatgttcag cgtccggcgt ttgtgttccc ctccttctca aacatcgcct
     3001 cgtccagcgg caactttcgc aacgccgcct catgcaggaa ggtcggtttt tggcctgtcc
     3061 ccgtgaacac gccgtccaga ctgccatccg gaccgatccc ccgcacgtca aaccggtaga
     3121 gatcctgcag gacatactcg ccatccttca ggcccaacac ctccgagatc gccgtgattt
     3181 tgcgcgagcc gtcggacagg cgggctgtgt gaaccaccac gtcgatggcg gaggcgactt
     3241 gggagcgcag ggcggagagc ggaatatcga tgccacttag aagcgagcag gtttcgatac
     3301 gcgagaggct gtcgcgtgaa ctgttggcgt ggatcgtcga catactcccc gcgtgtcccg
     3361 tgttcagtgc ctgcagcaga tccagcgctt cgccgccgcg aatctcgccg ataaccaacc
     3421 gatccggccg caaccgtaac gcggagtgga tcagatcgcg aatcgtcacc tcgcccttgt
     3481 cgtttttgtc cggcttacgt gtctcgaagc aaaccgtgtg cgtctgttgc aactgcaact
     3541 cactcgcgtc ctcaatgacc agaatccgtt cgttttcggg tatgaagccg ctgagcacat
     3601 tcagcaccga ggtcttgccg gaggaggtcg cgcccgacac gatcacgttc ttgtgcagtt
     3661 taacgatcgc gtcgatgagc tgcgccccgg ctggcgtgat gctgccgaaa gcgatcaact
     3721 gctccatggt gagcttttcc ttcttgaact tgcggatcgc gaccacggtt ccgcaccgcg
     3781 agagcggcgg gatcacggca tgcacgcgcg acccgtccgg cagccgggca tccagccgcg
     3841 ggttctcttc attgagtagg cggcccacat tcttggcgac attgacggcg gcggccttga
     3901 gcgccggttc gctgacaaac cgcgcctccg tggcttgcaa cctgccttta agttcgatgt
     3961 atacctgatt cggcccgttg atcaaaatct ccgaaaccgc atcatcctgc agaaacggtc
     4021 ggaccggctc gaaaaacaac gcgagatagg tattcggatc gtccatgatg tgagagcatg
     4081 ccgcatctgg atgaaccctg tcaacatcga aatcgatatc ggtatcggga tcgctatcgg
     4141 tatcgggatc gggagcgtcg acccgcgacc cgcgacattc gacccgcgac gatcga
//
LOCUS       DAOSRB010000236         4169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_19235_length_4169_cov_6.878950, whole genome shotgun sequence.
ACCESSION   DAOSRB010000236 DAOSRB010000000
VERSION     DAOSRB010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19235_length_4169_cov_6.878950"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1409
                     /locus_tag="PLZ74_10075"
     CDS             210..1409
                     /locus_tag="PLZ74_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HOR98705.1"
                     /translation="MALPRQNTNIPKIALFLSLNFKAHRDCLSGILDYVRTHKPWHLH
                     LMEGRRGGQGLIQIARWGCNGIIGSALHDDVVRLIRRAQVPSVVLMPPPALRAPSHPL
                     AACSCLTYDSETIGRRAAEYFLKGAYQNFAYVREPLNLFWSKVRGEGFAARLRQAGFA
                     CHVYETGSTQAKHDWAVEQPRLTAWLQALPKPVAVFASMDERGRQVIDACLEAGIRVP
                     EHVAVLGVDNDDLLCEATFPPLSSIETDMRKKGYQVAAHLESLLRNRHTRPRVLKVKP
                     VRVVLRRSSDTTAHAVNPLFSARAFIRETAGQAPIRVPDVARRIGGSRRFAEQRFKAL
                     FKTTIREEIERVRMEKVRELLRDTRIPLREVAKACQFAQAGYLGRRFKLHYGMTPGEY
                     RAGDKRS"
     gene            1457..1738
                     /locus_tag="PLZ74_10080"
     CDS             1457..1738
                     /locus_tag="PLZ74_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98706.1"
                     /translation="MERSALPKETAEEIRRRVFEEPGFMRVTQVTRRGGSVFRVSLRP
                     VALQDGRQYQGEMTEAGQTTVKNFTGDAARRGEVSKKCWDRPVRASCIS"
     gene            1699..2664
                     /locus_tag="PLZ74_10085"
     CDS             1699..2664
                     /locus_tag="PLZ74_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOR98707.1"
                     /translation="MLGQTGPRELHLVTASGDLHVRVTRKGKAMVSRSAPLTRKAEAY
                     PAHDRIKRQPLTAFDSGALLRVIGIAGADGRIKASMRGKYDQINEFLKVIDTVLDGRE
                     ERELRIVDCGCGRAYLTLAVHAYLTRVRGYTVRVCGIDRRASVVATARQLAEDLDVAG
                     EVTFLEGDIATCEPEFKPDLLLSLHACDTATDEALARGIEWTCRYLLCAPCCQHELHK
                     TLKEGGPMQAMMRHGILRERLADLLTDTFRAQILRVLGFRVRVVEFVSAEATARNLLI
                     RAEYCVKPGQAVAVAEYLALRDFWKVTPWLETRLQRLMEKHLARY"
     gene            complement(2658..4001)
                     /locus_tag="PLZ74_10090"
     CDS             complement(2658..4001)
                     /locus_tag="PLZ74_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98708.1"
                     /translation="MKRWTRVIGVWAGCALAVMAGNPTAAYIQRTMKALETSTAEAPA
                     HVRVLFYGQSIVAQGWTKTVQNQLRERYPTVRFEFQNAAIGGYGSQVLIRTADHDLYP
                     WYPDLLFFHVYGPTDKYEEIVRRTRERTSAEIVLWTSHLNSKEKAEELIAKPDERTRK
                     ILEVADTYRCLKIDLRKKWCEHLIAHNLPSTNLLTDSVHLKPEGCDLYARFIAEELVR
                     APELGDNPSASGTITTFGCNAPEVTRGADGSLTLRFTGNRVVALSDGTGAAGARARVL
                     LDGQPMAGKKELWAMTRPSRGPAWMPAINNIGFERPPIKETWTLQALPDSATNANPMH
                     FTLRGSVTGPDGEGWNTNRFVSPSGRVVIEKEDWGCIWQFKYFKKAMPKDFTVTWQAY
                     PLFAETYVPQPAGTRTLLMQGCDNGPHTLTLQPETEGALGISAFVVHAPPRSASQ"
BASE COUNT          772 a         1311 c         1285 g          801 t
ORIGIN      
        1 gtctcacagc ctttgagatt gcgggctccg cccgcatctc aatacgtcaa aacccaatgt
       61 ttacggggct ttgctagaat tccacctgtt caactgctcc ttttagtttc aatcgtatac
      121 gccttttttg cgtgtcattc ccatatcctt ttttgcgtaa gcggtggccg gtcttgcacc
      181 atatccccgc cttgcgtata ctttcccata tggctttgcc ccgtcagaac accaacatcc
      241 ccaaaatcgc gctttttctc tcgctcaact tcaaggcgca ccgcgattgc ctttcgggga
      301 ttctcgatta tgtgcgcaca cacaaaccgt ggcacctgca tttgatggag ggacgacgcg
      361 gcgggcaggg cctgatccag attgcccgct ggggttgtaa cggcatcatc ggcagtgcgc
      421 tgcacgacga tgtggtgcgg ctgatccgca gggcgcaggt cccctcggtg gtgctgatgc
      481 ctccgccggc gctgcgcgct ccgagccatc cgctcgcggc ctgttcctgc ctgacctacg
      541 actccgagac catcggccgc agggccgccg aatattttct gaagggtgcg taccagaact
      601 tcgcctatgt ccgcgaaccg ctcaacctgt tctggtcgaa agtgcgggga gaggggtttg
      661 ccgcccgcct ccggcaggcg gggttcgcct gccacgttta cgaaaccggc tcgactcagg
      721 ccaaacatga ttgggcggtc gagcagcccc gtctgaccgc ctggttgcag gccctgccca
      781 aacccgtggc ggtcttcgcc tccatggatg agcgcgggcg ccaagtgatc gacgcctgtc
      841 tggaggcggg tatccgcgta ccggagcatg tggcggtact gggcgtggac aatgacgatt
      901 tgctttgtga agcgaccttc ccgccgctct ccagcattga aacggatatg cgcaagaaag
      961 gatatcaggt tgccgcgcat ctggagagcc tgttgcggaa ccgccacacg cggccccgtg
     1021 tcctgaaggt caagcccgtg cgggtggtgc tgcggcgttc gagcgatacc acggcccacg
     1081 ccgtcaaccc gcttttcagc gcccgcgcat ttatccggga aaccgcaggt caggcaccga
     1141 tccgcgtacc ggatgtggcc cggcggatcg gcggatcgcg ccggttcgcc gaacaacgtt
     1201 tcaaagccct tttcaaaacc acgatccgcg aggagatcga acgggtccgg atggaaaagg
     1261 tccgggaact cctgcgcgac acccggattc cgttgcgtga ggtggcgaaa gcgtgccaat
     1321 tcgcgcaggc gggttatctc ggacgacgtt tcaaattgca ttacggcatg actcccggcg
     1381 aataccgggc cggggacaag cgttcgtagc ggagaggcat atccggagca aaagtcaaac
     1441 cgagcgaggt gaggagatgg aacggagcgc gttgcccaaa gagacggctg aggaaatccg
     1501 caggcgggtg tttgaggagc ccggcttcat gcgcgtgacc caggtgacgc ggcgcggcgg
     1561 gagcgtgttc cgcgtgagcc tgcgaccggt ggcgctccaa gacggacggc aatatcaagg
     1621 cgagatgacc gaggcggggc agaccacagt gaagaatttt accggcgatg ccgcgcggcg
     1681 aggcgaggtc tcgaagaaat gctgggacag accggtccgc gcgagttgca tctcgtgacc
     1741 gcctcagggg acctgcatgt gcgggtcacc cggaaaggca aggcgatggt gtcgcgcagc
     1801 gcaccgttga cgcgcaaagc cgaggcgtat cctgcgcacg accgcatcaa gcgtcagccg
     1861 ctgacggcct tcgactccgg cgcgctcctg cgggtcatcg gcatcgcggg tgcggatggg
     1921 cgcatcaaag cctccatgcg cggcaaatac gaccagatca acgaattcct caaggtgatc
     1981 gacaccgtgc tcgacggccg ggaggagcgg gaactgcgca tcgtggactg cggttgcggc
     2041 cgggcctatc tgacgctggc agtccacgcc tatctgacac gcgtccgcgg ttacacggta
     2101 cgggtgtgtg gcatcgaccg ccgggccagc gtggtggcca cggcgcgcca actcgccgag
     2161 gatctggacg tcgccggaga ggttaccttt cttgaaggtg acatcgcgac ctgcgagccg
     2221 gagttcaaac cggatctgct gctgagcctg cacgcctgcg acaccgccac ggacgaggct
     2281 ttggcgcgcg gcatcgagtg gacgtgccga tacctgctct gcgcgccgtg ctgccagcac
     2341 gagttgcaca aaaccctcaa agagggcggc ccgatgcagg cgatgatgcg tcacggaatc
     2401 ctgcgtgaac gacttgccga cctcctgacc gacacgttcc gcgcacagat tctgcgcgtg
     2461 ctcggattcc gcgtccgcgt ggtggagttc gtctccgccg aggccacggc ccggaatctc
     2521 ctgatccggg ccgagtactg cgtgaaaccg ggtcaggcgg tggcggtcgc cgaatatctg
     2581 gcgttgcggg atttctggaa agtcaccccg tggctggaga cgcgcctgca gcgactgatg
     2641 gaaaaacacc tcgcccgtta ctgagacgcg ctccggggcg gcgcatgcac cacgaacgcg
     2701 ctgatgccca atgccccctc cgtctccggt tgcagcgtca gagtgtgcgg cccgttgtcg
     2761 cagccctgca tcagcagcgt gcgcgtcccg gccggttgcg gcacataggt ctcggcgaac
     2821 agcggatagg cctgccacgt gaccgtgaaa tccttgggca tcgctttctt gaaatacttg
     2881 aactgccaaa tgcagcccca gtcttccttc tcgatcacga cccgtccgga tggcgacacg
     2941 aaacggttgg tgttccatcc ctcgccatcc ggccccgtca cactgccgcg aagcgtgaag
     3001 tgcatcggat tcgcattggt cgcggaatcc ggcagcgcct gaagcgtcca cgtctccttg
     3061 atcggggggc gttcaaaacc gatgttgttg atcgccggca tccacgccgg gcctctgctc
     3121 ggccgggtca tggcccacaa ctctttcttt cccgccatcg gctgaccgtc cagcaagacg
     3181 cgggcgcggg cgccggccgc gccggtgccg tcagagagcg cgaccacgcg gttaccggtg
     3241 aaacgcaggg tcaggctgcc atccgcgccc cgtgtcacct cgggcgcgtt gcagccgaaa
     3301 gtcgtgatcg tgccggaggc ggacggattg tctcccaact ccggcgcgcg caccaactcc
     3361 tcggcgatga accgggcgta gagatcgcac ccttcgggct tcaggtgcac cgagtcggtc
     3421 agcaggttgg ttgaaggcag attgtgggcg atcaaatgct cgcaccattt cttacgcaga
     3481 tcgatcttga ggcaacggta ggtgtccgcc acctcgagaa tcttacgcgt gcgctcatcg
     3541 ggtttggcga tcagttcctc cgccttttcc tttgagttga gatgactggt ccagagcacg
     3601 atttccgctg acgtgcgctc gcgtgtgcgc cggacgatct cctcgtactt gtcggtcggc
     3661 ccgtacacgt ggaaaaagag cagatccgga taccaagggt acagatcgtg atccgcagtg
     3721 cggatcagca cctgcgatcc atacccgccg atcgcggcgt tttggaactc gaatcgcacg
     3781 gtgggataac gttcccgcaa ttggttctgc acggtcttgg tccacccctg cgccacaatg
     3841 gactgaccgt agaacagcac ccgcacatgc gcgggagcct cggccgtgga cgtttccagc
     3901 gccttcatgg tgcgctggat atacgccgcc gtggggttcc ccgccataac cgcgagcgca
     3961 cagcccgccc aaacgccgat cacccttgtc caacgtttca tcaatccccc tgtttttctc
     4021 tcttccacat tgttgtgcag agtccgcatc atactccttt gaccagcgcg gcggcaagtt
     4081 ctaacaacga gacaagccag aattaacaat ccaaatcagt atcgctatcg ggatcgctat
     4141 cgggatcgat catttaacca cggaacact
//
LOCUS       DAOSRB010000237         4127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_19474_length_4127_cov_6.218811, whole genome shotgun sequence.
ACCESSION   DAOSRB010000237 DAOSRB010000000
VERSION     DAOSRB010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19474_length_4127_cov_6.218811"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /locus_tag="PLZ74_10095"
     CDS             complement(<1..1890)
                     /locus_tag="PLZ74_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98709.1"
                     /translation="MSIGSWVHKNNLAKGMVVCGLLQIAGYYVAGSLATDGFHLPAPQ
                     PDTLLYCQSARQVAEGHPFVYTPGDRPSTGSTSHLYPFALGLLYKLGAKGDALLTAGF
                     ALNALLYLLFLVSWAGIIVRQIRSPRNRFAACALVALSGQAAYSALSQSDAGLFMALS
                     SGLLFTLLSGRMAGFAVLLGLSPWCRPEGVLLSVLFAAALVFKRWGMRQRVTRQEGVA
                     AVVGLLSSAGLFAFNYGLTGHAQFLSVIHKGYAKQYDFFSALHLTLGDMVRILRELLL
                     GLPDALPRESFYVPLIGALCAWIGLVTLPRRREGLWKEVWWLSACAAGVGVVASSGWQ
                     NTNVDRYLAWLMPVWLVYTAAGAMRVGRGWGRTAVCRWGLLLIVAAFQMLNAAWLVTL
                     YHTACLITHPCYEFAKEAHARLPPGARVGGAVCNLAYGMPGRSYAHLSGLYSPDWMTP
                     EVVTNLERLKHHPERRFDYWALPSAQTEFGGSDIAVLCGPVAALGVDENQMRQAVWSP
                     LDGARAPHLADKEVRGWRLADRVDVGYAEEERRCGFAAESRYHGVTYIPFAMGTNTLF
                     DVGRMVVGWVSFSPRLTPGKPVRVIVRTAERVETEVRIGVRARRQTFTFVTPLQLRVR
                     VDGADA"
     gene            complement(1902..3041)
                     /locus_tag="PLZ74_10100"
     CDS             complement(1902..3041)
                     /locus_tag="PLZ74_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98710.1"
                     /translation="MAYAKIPEKDAVLLIRALGLLISQASVYGPSHKVTQNAVRSMFT
                     ELEQAVKTHGVIEIALNGRQLLVNGSSDGITEAVGKNVADRMALHKIGGLLFQAPAVL
                     EEFLICVTLLGTPPGALAAENGFEGAMRKAGLRSIQVVTVAFQRVTGERNEEPDLSHT
                     PLPADPQTATNRMRTGAGGIAVLDLSEALVLDDTAETPQTDLDTRAAKRKKHTSSLAS
                     LLRETAALLERDGDPPVGERHQAMIDALEQIRHVLSTLTVDSERHITSLAEQVNDDRQ
                     TIASIESAARRRGIGLKLTRAELIDRYAELNQEIVQPLTVSTGVIEMLKTKRAGALTP
                     LQCDLIKMAEESIERVNQLVAYMSRISGLPESLTPDSRIISESYR"
     gene            complement(3080..>4127)
                     /locus_tag="PLZ74_10105"
     CDS             complement(3080..>4127)
                     /locus_tag="PLZ74_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98711.1"
                     /translation="MIVSGMLARSATRQMRATQITREMLKARLIAESGLNKAYNAVKT
                     DFDRLNGFTETLAFGGGSCTVRAEMLTSEDGRERARLVSEGVCGLGRARVSADLENVT
                     RVLGDDQAGSVAGDYFDLLFDLLVGGILDLKGNFKATVTDIHANGNVKLTGSSNVDAM
                     RVSSTGTIEWKKNDGTVTLLTNQSPVEILSAALTEAINAFIAVAEENGAVYARGSDIP
                     SSPPGGVAVCTGSSDGWSKTGTGTFIFLGDVKLQGSGVNLTAQNGYPALIVLGTGEVK
                     INANAVIRGAVIMPNASFHLNGHAVIYGAVLIGQGMTGNGTADLYAGDSGQGFSLPPT
                     HQVEQDYVVVTAWH"
BASE COUNT          823 a         1430 c         1159 g          715 t
ORIGIN      
        1 cgcatcggca ccatccaccc gcacgcggag ttgcagcgga gtgacaaagg tgaaggtctg
       61 ccgtctggcg cgcacgccga tccgcacctc ggtttccacg cgttcggcgg tccgaacgat
      121 cacgcgcacc ggtttgcccg gcgtcagccg cggcgagaaa ctcacccacc ccaccaccat
      181 acggcccaca tcgaacaagg tgttcgtgcc catcgcaaag gggatgtagg tcacgccatg
      241 atagcggctc tccgccgcaa acccgcatcg ccgctcctcc tccgcatagc cgacatccac
      301 ccgatccgcc aggcgccagc cccggacttc cttgtccgcc agatgcggcg cgcgcgcgcc
      361 atcgagcgga gaccacacgg cttgccgcat ctggttctca tccacgccca acgcggccac
      421 cgggccgcac agcacggcga tgtcggaacc gccgaattcg gtttgcgcgg agggcagcgc
      481 ccaataatcg aaccggcgtt cgggatggtg cttgagccgt tccagattcg tcacaacttc
      541 gggcgtcatc caatccggcg aatagagtcc ggaaagatga gcgtaactcc gtcccggcat
      601 cccgtaggcg agattgcaga ccgccccgcc gacccgcgcc ccgggcggca gccgcgcatg
      661 cgcctctttg gcgaattcgt aacaggggtg ggtgatcagg caggccgtat ggtatagggt
      721 gaccagccag gccgcgttca acatctgaaa cgcggccacg atcagcaaga ggccccagcg
      781 acaaaccgcg gtccggcccc atccccgtcc gacccgcata gcacccgcag cggtatacac
      841 cagccacacc ggcatcagcc atgccagata gcggtccaca ttggtgttct gccatccgct
      901 cgaagcaacc acccccaccc cggccgcaca ggcgctcagc caccatacct ctttccagag
      961 cccctcgcgc ctccggggta aggtcaccag cccgatccac gcgcaaagcg ccccgatcag
     1021 cggcacataa aaactttcgc gcggcagagc atcgggcagg ccgagcaaca actcgcgcag
     1081 gatccgcacc atgtcgccca gcgtcaagtg cagggccgaa aagaaatcgt actgcttagc
     1141 ataccctttg tgaatcaccg acaggaactg ggcgtgcccc gtgagtccgt aattgaatgc
     1201 gaaaaggccg gcggaggaga ggagccccac cacggccgcg accccctcct gacgcgtcac
     1261 cctttgacgc atgccccacc gcttgaacac cagagccgcg gcaaacagta cggagaggag
     1321 caccccttcg ggacggcacc acggcgacag acccagcagc accgcgaaac cggccattct
     1381 cccactcagc aaggtgaaga ggagtcccga actcaatgcc atgaaaagcc cggcatccga
     1441 ttgcgacagc gcactgtaag cggcctggcc gctcagcgcc accagcgcgc aggccgcgaa
     1501 ccggttacgc ggggaacgga tttgccgtac gatgatcccg gcccaactca cgaggaacaa
     1561 gagatacaac agggcgttca gcgcgaatcc ggcagtcagg agcgcgtcgc ccttggcccc
     1621 gagtttataa agcaaaccca gcgcaaaggg gtagaggtgc gaggtgctgc ccgtggaggg
     1681 gcggtcgccc ggcgtataaa cgaaagggtg cccctccgcc acctgtcgcg cggactggca
     1741 gtagagaagg gtgtccggct ggggcgccgg cagatgaaaa ccgtccgtcg ccaacgagcc
     1801 ggccacataa tagccggcga tctgcaacag cccgcacacc accatcccct tggccagatt
     1861 atttttatga acccaagatc cgatgctcat ttttcatttt gtcagcggta cgattccgaa
     1921 atgatacgcg agtccggtgt gagggattcg gggaggcccg agatccggct catatacgcc
     1981 accagttgat tgacacgttc aatgctctcc tccgccatct tgatcagatc acactgcaag
     2041 ggggtcaacg cgcccgcgcg tttcgttttg agcatttcga tcacaccggt cgagaccgtc
     2101 agcggctgca cgatctcctg attgagttcg gcgtagcgat caatcagttc ggcacgggtg
     2161 agcttcagac cgataccgcg gcggcgcgca gccgattcga tgctggcgat ggtctgacgg
     2221 tcgtcattca cctgttcggc cagcgaggtg atgtggcgtt ccgaatcgac cgtcagagtc
     2281 gagagcacat gacggatctg ctccagtgcg tcgatcatcg cctggtggcg ctcgccgacg
     2341 ggcggatccc catccctttc caataatgcc gcagtctcgc gcaacaggga ggccagagag
     2401 gacgtgtgct ttttacgttt cgccgcacgc gtgtccagat cggtctgcgg cgtttccgcc
     2461 gtgtcgtcca gcacgagggc ttccgacaga tccagcacgg cgatgcctcc ggccccggtc
     2521 cgcatccggt tcgtggccgt ctgggggtct gcaggcagcg gcgtgtggga tagatcgggc
     2581 tcctcgtttc tttcccccgt gacgcgctga aacgcgaccg tcaccacctg aatgctccgc
     2641 aggccggctt tacgcatcgc gccttcgaac ccgttctctg cggccagcgc gccgggcggc
     2701 gtacccaaca acgtcacgca gatcaaaaac tcctccagca cagcaggcgc ctgaaaaagc
     2761 aagccgccga ttttgtgcag ggccatgcga tccgccacgt tcttgccgac cgcttccgtg
     2821 ataccgtcgg acgagccgtt gaccagaagt tgccgcccgt tcaacgcgat ctcgatcacg
     2881 ccgtgggttt tgaccgcctg ctccaattcg gtgaacatgc tgcgcaccgc attctgggtc
     2941 accttgtggg aaggcccgta cacgctggct tggctgatca gcaaccccag cgcccggatc
     3001 agcagcactg cgtccttctc cggtattttc gcatacgcca tgccttatcg ccctctcccg
     3061 cttcccacaa gcagccgcct taatgccaag ccgtcaccac cacatagtcc tgctctacct
     3121 gatgcgtagg cggcaagctg aacccttgtc cactatcccc ggcatacaga tccgccgtgc
     3181 cgtttccggt catcccctga ccgatcaaga cggccccgta gatcacggca tggccgttca
     3241 gatggaaact cgcgttcggc atgatgaccg cgccgcggat cacggcattg gcgttgatct
     3301 tgacttcacc cgtacccagc acaatcaggg cagggtatcc gttctgggcc gtcagattca
     3361 ccccgcttcc ctgcaatttg acatccccca gaaagatgaa cgtaccggtt ccggtcttgc
     3421 tccacccgtc tgaagaaccg gtacagaccg ccacgccccc ggggggcgag gagggaatgt
     3481 ccgaaccgcg cgcgtaaacc gcaccgttct cctccgcaac cgcgatgaac gcattgatcg
     3541 cctcagtcaa cgccgcactc aagatctcga ccggcgactg gttggtaagc aacgtgaccg
     3601 taccgtcgtt cttcttccac tcgatggtgc ccgtgctgga cacccgcatc gcatccacgt
     3661 tcgaactccc cgtcagcttt acgttaccgt tggcgtggat atctgtcacc gtggccttga
     3721 aattgccttt gaggtccaga atcccgccga ccagcagatc gaagagcaag tcaaaataat
     3781 cgcccgccac actgcccgcc tgatcgtccc ccaacaccct cgtcacattc tccagatcag
     3841 ctgaaacgcg cgcgcggccg aggccgcaaa ccccctcgga gacgagccgc gcccgctcgc
     3901 gcccgtcctc cgaagtcagc atctcggccc tgacggtaca ggacccgccg ccgaaggcta
     3961 aggtttcagt aaacccgttg agccgatcaa agtccgtttt cacggcgtta taggccttgt
     4021 tcaggcccga ctccgcgatc agccgcgcct tgagcatctc gcgtgtgatc tgcgtggcgc
     4081 gcatctgccg cgtcgccgag cgggccagca tgccggagac gatcatg
//
LOCUS       DAOSRB010000238         4124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_19490_length_4124_cov_7.053084, whole genome shotgun sequence.
ACCESSION   DAOSRB010000238 DAOSRB010000000
VERSION     DAOSRB010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19490_length_4124_cov_7.053084"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLZ74_10110"
     CDS             <1..215
                     /locus_tag="PLZ74_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98712.1"
                     /translation="PRFRHRRSLFRVSGFLVSGFQGFKVSGFIGVGIAIGIGIGVGIA
                     IGIGVDIRSDGRSGRPCPLADALGAP"
     gene            394..1677
                     /locus_tag="PLZ74_10115"
     CDS             394..1677
                     /locus_tag="PLZ74_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOR98713.1"
                     /translation="MRKLLVIQVAGWGHAFSERHGVRRIAGLPVCSSEAVFPALTCVA
                     QAALRTGLDPAGHGILANGFFDRTLRKAWFWEQAATLVEGPRIWEAFRGCGGTVGLAY
                     FQQSLGESADQILSPAPIHKHGGGMILAHYSRPPDLEARLTRAAGGPFRLCRYWGPLA
                     SVGSSEWITRAVAAWLAQPDAPDLVFAYLPGLDYDLQRFGPEHPKSVRALTVVCAEIE
                     RLFAAARANGYEALAFGDYAITPVTHGPVFPNRALRDAGLFAVRAVRGRAYPDFYQSR
                     AFALADHQIAPVFCPDPVALPQTRAVLETLNGVDRVLDSAEQAALGLAHARAGELLLV
                     AKPGAWFAYPWWTDPREAPDYAGHVDIHNKPGYDPCELFFGRMPLRTGADADRIRGTH
                     GLTGAGYETAWVSTVPFSFKTLPLLAASIRAWLAA"
     gene            1674..2855
                     /locus_tag="PLZ74_10120"
     CDS             1674..2855
                     /locus_tag="PLZ74_10120"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOR98714.1"
                     /translation="MSNLIQKFTVSYRYPVCFTRRVFDPSNQTLAKILKPSASGRARA
                     LVVVDAGVAEVAPGLPQRIERYFRLRCGKIDLVRAPMRVPGGEQAKNGWAGVRALMTL
                     AAETHLDRHSYLVALGGGSVLDMAGFAASLVHRGLRLIRLPTTVLAQGDAGIGVKNGM
                     NSGEVKNAVGTFAPPFAVINDFDFLPTLPQREWVSGIAEAFKVAIIQDRRFFRFLLKH
                     AVALRGRDQALMETLVRKTATLHLRHIATSGDPFEYGSARPLDFGHWAAHRLESLSNF
                     TFGHGQAVAVGIALDSTYAMLNGLISPSERDAILQGLTASGLPVWTPLLERSGANGAP
                     DVLVGLEQFREHLGGKLCVTLPNPLGAKTEVRSIRLPLMRKAIGLLRPGERRMEKLGK
                     A"
     gene            2895..3257
                     /locus_tag="PLZ74_10125"
     CDS             2895..3257
                     /locus_tag="PLZ74_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98715.1"
                     /translation="MFAQPAKRVGAIPVAWRQSVCAALAERGECVRLTVRARHEWEAA
                     FPNAFEYELYAVLEATLKKGTVEGRRVEDMRPEGEAYEFFFEFEGRKVYGKINLLTPE
                     RRIVLIISAHIPNKGDTL"
     gene            3254..3805
                     /locus_tag="PLZ74_10130"
     CDS             3254..3805
                     /locus_tag="PLZ74_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98716.1"
                     /translation="MKRLAPKDETPPFAGLERAKTKLPVIIPAPEGSGLEPRVLEVEV
                     NGWRDPTDGEIYLSGTTTAYVDQIKAHEMGLLAPQDFKRIREQLKVTQEEISSLLQIG
                     KRSWTRWETGRDRPTRSINLLVRSLYDGIITVPYLEELQKPIDHEPLTRWRCTVCPSL
                     YSAPKKQRAQVCEEVSDEVGPAA"
     gene            3783..>4124
                     /locus_tag="PLZ74_10135"
     CDS             3783..>4124
                     /locus_tag="PLZ74_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98717.1"
                     /translation="MKSVPPLECLGYFVTALTVSANPKHNPEAKVELTTDDLTVTTKC
                     VCTDKAARRWHVQLTIRQAVGPDKNAPYNFRLVLLGEFEVVSGFPKEHERRLVETNAC
                     SILMGSAREVLR"
BASE COUNT          713 a         1174 c         1388 g          849 t
ORIGIN      
        1 gcccgcgttt ccgacaccgc aggtcgcttt tcagggtttc agggtttctg gtttcggggt
       61 ttcagggttt caaggtttca gggttcatcg gggtcgggat cgctatcggt atcgggatcg
      121 gggtcgggat cgctatcggg atcggggtcg acataaggtc tgacgggaga agcgggcgtc
      181 cctgcccgtt ggcggacgcc ttgggcgcac cgtaaaaccc aaagtttacg gggctttgct
      241 agaactctac ccgttcaact gctccttttg ggttcaatgc cccttggttg ttggtgtcgc
      301 gcttgtcatc ggtaacgggg cgcattatac ttctgacttc agcggatcgg gtcggctcaa
      361 cgcggtgccg acgtcgaaaa cgggaaacag gacatgcgca aactgctggt tattcaggtc
      421 gccggatggg ggcacgcgtt ctccgaacgt catggcgttc ggaggatcgc ggggttgccg
      481 gtctgctctt cggaggcggt ttttccggca ctgacctgtg tggctcaggc cgcgttgcgc
      541 accgggctcg atccggccgg gcatgggatc ctcgccaacg gtttttttga ccggaccctg
      601 cgcaaagcct ggttctggga gcaggcggcc acgctggttg aggggccgcg gatttgggag
      661 gcgttccgtg gctgcggcgg gactgtgggt ctcgcctact ttcagcagag tctcggagaa
      721 tcggcggatc agatcctctc gcccgcgccg attcacaagc atggcggcgg gatgatcctg
      781 gcgcattaca gtcgtccgcc ggacttggaa gcgcggctga cccgggccgc gggcggcccg
      841 ttccgcctgt gccgttattg ggggccgctc gcgtcggtcg gttcttcgga gtggatcacg
      901 cgggccgtgg ccgcatggct tgcgcagccg gacgcgccgg atctggtttt cgcgtatctg
      961 cccggactcg attacgatct ccagcggttc gggccggagc atccgaaaag tgtgcgggcg
     1021 cttacagtgg tttgcgcgga aatcgaacgg ctctttgctg cggctcgcgc caacgggtat
     1081 gaggccctgg ccttcgggga ttacgcgatc acgccggtga cgcatggccc ggttttcccg
     1141 aaccgtgcgt tgcgtgatgc ggggttgttc gccgtgcgcg cggtccgcgg cagggcgtat
     1201 ccggattttt accagagccg cgctttcgcg ctcgccgacc accagatcgc gccggtgttc
     1261 tgcccggacc ccgtcgccct gccgcagacg cgggccgttc tggagacgct gaatggtgtg
     1321 gaccgggtac tggactcggc agagcaggcc gcgctcgggt tggcccatgc gcgcgcgggc
     1381 gaactgttgc tggtggcgaa acccggcgca tggttcgcct atccctggtg gaccgatccc
     1441 cgtgaagcac cggattacgc gggtcatgtg gacatccaca acaaacccgg ctatgatccc
     1501 tgtgaactct ttttcgggcg catgccgctg cggaccggcg ccgatgccga ccgcattcgc
     1561 ggcacgcacg gactcaccgg cgcgggttac gaaaccgcat gggtctcgac cgtgccgttc
     1621 tcattcaaaa ccttgccgct tttggcagca tccattcggg cgtggcttgc cgcatgagta
     1681 acctgatcca gaaatttacg gtttcgtatc ggtatccggt gtgctttaca cgacgggtct
     1741 tcgatccgtc caaccagact ctggccaaga tactgaaacc gtcggccagc ggccgggcgc
     1801 gtgcgttggt ggtggtggat gccggggtgg ccgaagtcgc accggggcta ccccagcgta
     1861 tcgagcggta cttccgtctg cgttgcggga aaatcgatct ggtgcgcgct ccgatgcgtg
     1921 ttccgggcgg agaacaggct aagaacgggt gggccggggt acgcgcgctg atgacccttg
     1981 ccgcggaaac ccatctggac cggcacagtt atctggtcgc gttgggcggt ggcagcgtgc
     2041 tggacatggc gggtttcgcc gcctcgctgg ttcaccgcgg cctgcgcctg atccgtctgc
     2101 cgaccaccgt gctggcgcag ggcgacgcgg gcattggggt gaagaacggc atgaacagcg
     2161 gggaggtgaa aaatgcggtg ggcacctttg ccccgccgtt cgcggtgatc aatgatttcg
     2221 acttcctgcc aaccctgccg cagcgcgagt gggtcagcgg catcgcggag gcgttcaaag
     2281 tcgcgatcat tcaggaccgg cgctttttcc gttttctcct gaagcatgcc gtagccttgc
     2341 gcgggcgcga tcaggcattg atggagacgc ttgtccgcaa aaccgccacg ctgcacctgc
     2401 gccatatcgc cacgtccggc gatccctttg aatacggcag cgcgcggccg ctggatttcg
     2461 ggcattgggc cgcgcaccgg ctggagagcc tgtcgaactt cacgttcggg cacggccagg
     2521 ccgtagctgt cggcatcgcg ctcgacagca cgtacgcgat gctcaacggg ctgatctccc
     2581 cctcggaacg cgacgccatc cttcaggggt tgaccgcgag cgggctgccc gtctggacgc
     2641 cgctgctgga acggtctggc gcgaacggcg cgccggatgt gctcgtcggc ctcgaacagt
     2701 tccgcgaaca ccttggcggc aaactctgcg tcacgttgcc caacccgctc ggcgcgaaaa
     2761 ccgaagtgcg ctcgatccgt ctgccgctca tgcggaaggc catcggctta ctgcgccccg
     2821 gtgagaggcg catggagaaa ttgggaaagg cttgattttt acggcttgct gccggacgta
     2881 tcaattggta cattatgttt gctcaaccag ccaaaagagt gggcgccatt ccggttgcgt
     2941 ggcggcagtc cgtctgcgcc gcgctggcag aacgcgggga gtgcgttcga ctgacggtga
     3001 gggccaggca tgagtgggaa gcggcgtttc ccaacgcatt cgagtacgag ttgtatgccg
     3061 tgctggaagc gacgttgaaa aagggaacgg tggaagggcg gcgtgtagag gatatgcgtc
     3121 cggagggaga agcctacgag ttcttcttcg aattcgaggg gcggaaggtg tacgggaaga
     3181 tcaatctgtt aacgcccgaa cggcgtattg tcttgatcat atcagcgcat attccgaata
     3241 aaggagacac actatgaagc gtcttgcacc gaaagacgag accccgccgt ttgcgggcct
     3301 cgaacgggcg aagacgaaat tgccggtgat cattccggcg ccggaggggt ccggtctgga
     3361 accgcgggtt ctggaggtcg aggtcaacgg gtggcgtgat ccaacggacg gcgagattta
     3421 cctgagcggc acgacgactg cttatgttga tcagatcaaa gcgcacgaaa tggggttgct
     3481 tgcgccgcag gatttcaaac ggatccgcga acaattgaag gtcacccaag aggagatttc
     3541 cagtctcttg cagatcggaa agcggtcctg gacacgctgg gagaccggac gtgatcgtcc
     3601 gacccgatcg atcaacctgc ttgtccgttc cctctatgac gggatcatca cggtcccgta
     3661 tttggaggaa ctgcagaagc cgatcgatca tgagccgctc acgcggtggc ggtgcacggt
     3721 ttgtccaagt ctttatagcg ctccgaaaaa acaacgcgct caggtatgcg aggaggtgtc
     3781 tgatgaagtc ggtcccgccg cttgagtgtc tgggctattt tgtcaccgct ttgacggttt
     3841 cggcaaaccc taagcataac cccgaggcca aagtcgagct gaccactgac gacctcacgg
     3901 tcacaacgaa atgcgtttgc acggacaagg cggcgcggcg ctggcatgtc cagttgacga
     3961 tccgtcaggc tgtggggccg gacaagaacg ccccatacaa cttccggttg gtgttgttgg
     4021 gggagtttga ggtcgtaagc gggtttccta aggaacacga acggaggctt gtcgagacga
     4081 atgcgtgttc gatcttgatg ggttcagccc gcgaagtgtt gcgg
//
LOCUS       DAOSRB010000239         4121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_19514_length_4121_cov_7.374816, whole genome shotgun sequence.
ACCESSION   DAOSRB010000239 DAOSRB010000000
VERSION     DAOSRB010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19514_length_4121_cov_7.374816"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..450)
                     /locus_tag="PLZ74_10140"
     tRNA            complement(374..450)
                     /locus_tag="PLZ74_10140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(414..416),aa:Val,seq:tac)
     gene            586..1395
                     /locus_tag="PLZ74_10145"
     CDS             586..1395
                     /locus_tag="PLZ74_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOR98718.1"
                     /translation="MTDGFQLVVCGSLVPDPLQTLEPVSTPQGPGLKNEMMLPSVLDP
                     WAAHALYEAADLAKRFPGSKVWLVSLGPKAKLQQLMMTVAQKVPFELIALDGSASGFT
                     DALGVAEALAGAIEAIPGLDKSRLLVFGGCASASRDAGVTLQVVGERLGIRDQFVGVD
                     MLTVRPEGGFEVLERIEGGQYQVSVCAGAPAVLAWATGNLPEPPNNPQIGMANMRGVM
                     PALQKAKPAAAGVGGMAYGDVELPSQKRQTRIVKDATADEIAREIVEWIKA"
     gene            1416..2441
                     /locus_tag="PLZ74_10150"
     CDS             1416..2441
                     /locus_tag="PLZ74_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOR98719.1"
                     /translation="MTTVLALMHTQEDGSLPKAAREALTAASQLAAQSGAALSVGVIG
                     KGASAAADTVAGCGAASFWVIGDEAVYPARYATDAAAVTALVKAAGAQIVVAPGSSRF
                     MRCLPGASYRLGAVIDTHIVAQQAAPDGSVRVQRWFYRQRILGSFSRAARPWLLVVDS
                     GVNAPWEGAAGSASAQTVEVAFAEDGQRTTVTGARAPAADAQTIRPDAPLLFVAGAGW
                     TKKQKDGQPHIAEASGLIQTFLGKTQASLGSSKSLVDIQSEGQETLHFMSHMNQIGQT
                     GSTPRHPKGLATCCHGEEPHAVGWRFINERRAVNLDPNCGWAQGKADVVYVADAFDVM
                     ARVNELL"
     gene            2588..4054
                     /locus_tag="PLZ74_10155"
     CDS             2588..4054
                     /locus_tag="PLZ74_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOR98720.1"
                     /translation="MGVGGERGAVLPGGYREMWRIATPLILSMASYTIMQFCDRVFLA
                     RYSSVTIQAALPAGALAHTLICFFQALAGYAGTFTAHYYGANDPVQCVRATIQGIWLA
                     LGSWPLILLQIPLGCGIMTLFGHMPEVLEAEKTYFLILMAGGITVPLNAAVGGYFMGI
                     GKTSVNMKSNLIGCSLNVVLNYVMIFGKWGCPELGIAGAAYATLISSAVTCLLQGLPF
                     YQACRICMEERRNASRPDSSAGTLFSDFWFPDYRMLSRIVRYGTPSGLQLLMDVGSFA
                     VFIMLTGRLGEVALAASNIAFSINNLAFAPLLGFGLAASMLVGQHQGAGRSDAAERSG
                     YTGLKMGLAYMCVIGSTFVLFPHLYFELFRPPDAAFSTAELLGTGRVMLWLMTIWGVL
                     DTVSIVLSGALKGAGDTHFVMVYMIIGSWLFLVPGSLVLLRLGFGILGLWVWMAVYIS
                     VLASGFWWRWRTGRWKTIRVIDRREGLDYIPLVTDEIH"
BASE COUNT          721 a         1083 c         1461 g          856 t
ORIGIN      
        1 cccccgaacc gaggcggatg cgtcaggcgg cgcgtgtgtg cgtgattatc ggcacgggcg
       61 caaagctggg gcttccgaga tcggcaaggg ctggggctgg ggggctgttt tcgcgtttgt
      121 gtaattccaa aacacaaacg gcgggggcaa aggtcggcat gtgaagccat taaaagcatg
      181 aaaactaagc gggcgtgaat attgttgatt ttgggcgttt gtgtttgtgt gtgtgtttgt
      241 gtgtgtaaat gacccctata aatcagcttg tttcagattg tagcagggca aagggcaacc
      301 atgccgtata agcagaaaac cccgctttta ttgggggttt ttaatgaaaa tgagccgcaa
      361 acgctcttaa agctggtggg cggtgaggga ctcgaacccc cgacattctg ggtgtaaacc
      421 agactctcta accaactgag ataaccgccc cgaaaagcgt gcgcattatg ccgtttcatg
      481 cggaccggtg gcaagaaccg aatttaccgc ctgcggcgcg ggcggaaaaa gggcattcca
      541 cccggtgagg gccatctgct atagtgtgtg gcaaaggaga gaacgatgac agacggattt
      601 caacttgtcg tgtgcggtag tctggtgcct gatccgctcc agacgctcga accggtgtcc
      661 accccgcagg gtcccggatt gaaaaacgaa atgatgctgc cttcggtgct ggacccgtgg
      721 gcggcgcatg cgctgtacga ggcagcggac ttggcgaagc ggtttcccgg cagcaaggtc
      781 tggctggtga gcctcggccc caaggccaag ttgcagcaat tgatgatgac cgtggcccag
      841 aaggtgccgt ttgagttgat cgcgctggac ggcagcgcca gcggcttcac ggatgcgttg
      901 ggcgtggcgg aggctttggc cggggccatt gaggcgattc ccggtctgga caaatcccgc
      961 ctgctggttt tcggcggatg cgcgtcggcc tcgcgtgacg cgggtgtgac gttgcaggtg
     1021 gtgggcgagc ggttgggcat ccgcgatcag tttgtcggcg tcgatatgct gaccgtgcgg
     1081 cccgaaggcg gtttcgaggt gttggaacgg attgagggag ggcaatatca ggtttcggtt
     1141 tgcgcggggg cgccagcggt tttggcttgg gcgaccggca acctgccgga accgccgaac
     1201 aatccgcaga tcggcatggc caacatgcgc ggggtgatgc ctgcgctcca aaaggccaaa
     1261 cctgcggcgg cgggcgtggg cggcatggcc tacggggatg tcgaactccc gtcgcaaaaa
     1321 cgccagacga ggattgtgaa ggatgcgacg gcggatgaga tcgcccgcga aatcgtggaa
     1381 tggatcaaag cgtagcggaa cagggaggat tggatatgac aacggttttg gcgttgatgc
     1441 acacgcaaga ggacggcagt ctgcccaaag cggcgcggga ggcgctgacg gccgcgagcc
     1501 aactcgccgc gcaatccggc gcggcgctgt cggtcggtgt gatcggaaaa ggggcgtcgg
     1561 cggctgctga tacggtggcg ggctgcggtg ccgcgtcgtt ttgggtgatc ggcgatgagg
     1621 cggtgtatcc ggcgcgctac gcgacggatg ccgcggcggt cacggctttg gtcaaagcgg
     1681 cgggcgcgca gatcgtggtg gcgccgggga gttcgcggtt catgcggtgc ctgccgggcg
     1741 cgtcgtaccg tctgggtgcc gtgatcgaca cccacatcgt tgcgcagcag gcggcgccgg
     1801 acggttcggt gcgcgtgcag cgctggtttt accgtcagcg gattttgggt tctttcagcc
     1861 gtgcggcgcg gccgtggctg ctggtggtgg atagcggggt caatgcgccg tgggagggtg
     1921 ccgccgggag cgcgtcggca cagacggtgg aggtcgcgtt tgcggaggat gggcagcgca
     1981 cgacggtcac gggggcccgc gcccctgcgg cggacgcgca gacgatccgt ccggacgccc
     2041 cgctgctgtt tgtggcggga gccgggtgga cgaagaagca gaaggacggc cagccgcata
     2101 tcgcggaggc cagtgggttg atccagacgt ttctggggaa gacacaggcc tcgctcggca
     2161 gcagcaagtc gctggtggac atccagagcg agggtcagga gacgctgcat ttcatgtcgc
     2221 atatgaacca gattggccag acgggttcca caccgcgtca tccgaaaggg ttggcgacct
     2281 gctgccacgg cgaagagccg catgcggtgg gctggcgttt catcaacgaa cgccgcgcag
     2341 tcaatctcga tccgaattgc ggatgggcgc agggcaaggc ggatgtggtc tatgtggcgg
     2401 acgcgtttga tgtgatggcg cgggtcaacg agctgttgta ggccgtgcgg gccgcaggcc
     2461 cggaccggca gtccggaggt gtggggcgac gcgcaggcgg atcgccccgc gctgtccggg
     2521 ttgccggtgt gatcctgtca gggtgataca gccagggtcg gaaggacggg gcccgggaag
     2581 ggggcgtgtg ggcgtggggg gagagcgtgg cgcggtgttg ccgggcggat accgggagat
     2641 gtggcggatc gccactccgc tcatcctcag tatggcgtcc tacaccatca tgcagttctg
     2701 cgaccgcgtg tttcttgcgc gttacagcag cgtgacgatt caagcggctt tgccggcggg
     2761 cgctctcgcg cacacgctga tctgtttctt tcaagcgctc gcgggatacg ccggcacttt
     2821 caccgcccat tactatgggg cgaacgatcc ggtgcaatgc gtgcgcgcca cgatccaagg
     2881 catctggctg gcgctcggct cctggcccct gatcctgctg cagattccgc tcgggtgcgg
     2941 catcatgacc ctcttcggcc atatgcctga agtgctcgag gctgagaaaa cgtacttcct
     3001 gattttgatg gcaggcggga taaccgttcc gttgaacgcc gctgtgggcg ggtacttcat
     3061 gggcatcggg aagacgagcg tcaacatgaa gtcgaatctc atcggctgtt cgctcaacgt
     3121 cgtgctcaat tacgtcatga tcttcggaaa atgggggtgc ccggaactcg gcatcgcggg
     3181 cgcggcatac gcgacactga tctccagtgc ggtcacctgc ctcttgcaag ggctcccgtt
     3241 ttatcaagcc tgccggattt gcatggagga gaggcggaac gcttcgcgtc cggattcttc
     3301 cgcgggaacg ctgttctccg acttctggtt tccggattac cggatgttaa gtcgcatcgt
     3361 gcggtacggc acgccatcag gtctgcagtt actgatggat gtcgggtcgt tcgccgtctt
     3421 catcatgctg acgggacggc tgggcgaggt ggcgttggcg gccagcaaca tcgcgttcag
     3481 catcaacaac ctggctttcg ccccgctgct ggggttcggg ttggcggcct ccatgctggt
     3541 ggggcagcat cagggggctg gacggtcgga cgcggctgaa cgatcgggat ataccgggct
     3601 caagatggga ctcgcttata tgtgtgtgat cggttccact tttgtactct tcccgcacct
     3661 gtattttgaa cttttccgtc ctcccgatgc ggcgttctcc actgcggaac tgttgggaac
     3721 cggacgcgtc atgctgtggc tcatgaccat ctggggagtg ctcgacaccg tgtcgatcgt
     3781 cttgagcggc gcgttgaaag gcgcgggaga cacgcatttc gtcatggtct acatgatcat
     3841 cggaagctgg ctgtttttgg ttcccggttc gctggtcttg ctacgcctcg gtttcggtat
     3901 cctggggttg tgggtatgga tggccgtcta tatttcggtt ctggcctccg ggttctggtg
     3961 gcgctggcga acgggccgtt ggaaaacgat ccgtgtgatc gatcgccggg aaggcctcga
     4021 ttatattccg ctggtcaccg acgaaattca ctgagcttgt tttagtctaa aaggagcagt
     4081 tgaacgggta gaattctagc aaagccccgc aaacattggg t
//
LOCUS       DAOSRB010000240         4097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_19666_length_4097_cov_5.762741, whole genome shotgun sequence.
ACCESSION   DAOSRB010000240 DAOSRB010000000
VERSION     DAOSRB010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19666_length_4097_cov_5.762741"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLZ74_10160"
     CDS             <1..289
                     /locus_tag="PLZ74_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98721.1"
                     /translation="CSILKILSSCQFVGKELGVFSLRHSEFGVGRSAFKGGGVKRRPD
                     EQTLVPPILRQNKKICAICKSADKTSSLKTRCCGQGRPRSLLTAPLFTRNA"
     gene            complement(357..2729)
                     /locus_tag="PLZ74_10165"
     CDS             complement(357..2729)
                     /locus_tag="PLZ74_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98722.1"
                     /translation="MKTRWMLLAVIWAAGTLSANDALRLSDDLCSLRFADGRLVIADK
                     TGTDILAVGKVPFLWSVPCAVPKSVQRAANGRLRIAYEITNASGTNFPFSAEAYLTGH
                     GFAVDFVSEIPPCFRSGGQMVEVFRLNDTGRSQQVTKCGYWQRCKPAVAEGWYDPGVP
                     FEVKSMNVKPYRAKSGQVFWCRNTGWSGARTECPGFPRTSTNGVYHGRLEFVTGVDSE
                     DAQCAAAQVDGRPFVIALATERPFNLFESGAPKFRLTVKPLIDGWRTVRTVARDFDGK
                     VVFDQSERFEFKIGVPFVRDYALPAVEEGARGIWFIESTVGEGEREDGFARMNVAVLP
                     PHRFTHRDTSIAAMASYPETPEAERLMERLGVSILRYGDNNHFRTNYGITAYASRHAP
                     PEMFDPQNPKHVKIIEKEIVGRIKERGNPYFEFGNEVGWHKTHEEQTRLVKCYVSWLK
                     VIRARLAAEGLGHVKIITFGIQPDYSAHMMRIMKDEGVFDLVDGLTLHPGRGYYTADV
                     THGGWVYRGIIQRARARFAELGYPDKEIHMTECYAATHPNDGWKDSLRQATENTLLSL
                     VIASVEPNVKNMMFYKLHQGTSQDPHGYPLAHPSGQTTVNAEYDFGLLMRDDSPKPSL
                     LSYAATCEHLDGAKFLGEKGAGTNRTDTLRAFVFDTPRGRLAVLFDRNEGGMLYKLWQ
                     RELPKGVYRDALFRHKDPWVRHWHRTKPYAFKTAGAVRVFDMIGRERPAAVKDGKFEI
                     ALDGEPVFVYGLDPVDILPAASFPAAPAVGREPSEDEYAAEEKSSGKVSD"
     gene            complement(2831..4033)
                     /locus_tag="PLZ74_10170"
     CDS             complement(2831..4033)
                     /locus_tag="PLZ74_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98723.1"
                     /translation="MNVPQATPQYTGRRYFEWRSGYSVSNEMVVSGSSVASGFSTFDM
                     NGAWDLLRIQDTAKASPPVVMNGVSNRVEVLGGELSSGSCQVNGRYNRLLLSDGLLVT
                     GNKGTLTMGGNGPNLIRQTGGTCSAPDGVTIGGFSNRYEFVGGTMDKSLTFSGTNGYF
                     SIGAKRANGVLTFGAASKDCLCELEAAWTNNAGQNAVVLGGTNNVFVLKDGTEYYTSG
                     DGSVISFAEKPNGRLVISNATYTMNGRVCVGSGNVDAFNWTNSPNAAIEFRGTNPLMR
                     CVSTPNWKAWVLGTASGQPLLDPVHFKFVIPEQGYAAAPMQNQVANRTATLYGNTVFE
                     IDGSAYKGTEPVPLFYTAGGWLDMSQTVVDQLNASAILPPSWTLVWDKGKTIYAKPPP
                     SGSVLTIR"
BASE COUNT          796 a         1358 c         1139 g          804 t
ORIGIN      
        1 ttgttcaatc ctgaaaatcc tgtcatcctg tcaatttgta ggcaaggagt taggagtttt
       61 ttcacttcga cattctgagt tcggcgtcgg gcgttcggcg ttcaaaggcg gcggcgtgaa
      121 acgccgcccg gacgagcaga cgctcgtccc tcccatcctg cggcaaaaca aaaaaatctg
      181 cgcaatctgc aaatctgcgg ataaaaccag cagcctcaag acacgttgtt gcgggcaagg
      241 acgcccacgc tcccttctca ctgctcccct tttcaccaga aacgcatgac cttcgcacag
      301 ccctgtcacg gaccgatagc ccgtcgtttc gcccgtttca aaacggaatt acccgatcaa
      361 tcgctcacct tgccgctcga cttctcttcg gcggcgtatt catcttcgct cggctcccgt
      421 ccgacggccg gagccgcagg gaacgacgcg gcgggcagga tgtcaaccgg atccagaccg
      481 tagacaaaga cgggttcgcc gtcgagcgcg atctcgaact tcccgtcctt gaccgccgcc
      541 ggccgttcgc ggccgatcat gtcgaagacg cgcaccgcgc ccgctgtttt gaacgcatag
      601 ggcttcgtgc ggtgccagtg ccgcacccac ggatccttgt gccggaaaag cgcgtcgcgg
      661 tagacgccct tgggcagttc gcgctgccaa agtttgtaca gcatgccgcc ttcgttccgg
      721 tcgaagagga ccgccagccg cccgcggggc gtgtcgaaga cgaatgcgcg cagggtatcc
      781 gtgcggtttg tgccggctcc cttctcaccg agaaacttcg cgccgtccag atgctcgcat
      841 gtcgccgcat agctgagaag ggacggctta ggcgagtcgt cgcgcatcag aagcccgaaa
      901 tcgtactcgg cgttcaccgt cgtctgcccg ctcgggtgcg cgagcggata gccgtgcgga
      961 tcttggctcg tgccttggtg aagcttgtag aacatcatgt tcttgacgtt cggctcgacg
     1021 gatgcgatga cgagcgacag gagcgtgttc tcggtcgcct ggcgcagcga atccttccag
     1081 ccgtcgttcg gatgcgtcgc cgcatagcat tcggtcatgt ggatttcctt gtctgggtaa
     1141 ccgagctcgg cgaagcgggc gcgggcacgc tggataatgc cgcggtatac ccaaccgccg
     1201 tgcgtcacat cggccgtata atagccgcgc ccggggtgaa gcgtcagccc gtccacgagg
     1261 tcaaagaccc cctcatcctt catgatccgc atcatgtgcg cggaatagtc cggctggatg
     1321 ccgaaggtga tgatcttgac gtgtccgagt ccttccgccg cgagccgggc acggatgacc
     1381 ttgagccaac tcacgtagca tttgacaagc cgcgtctgtt cctcgtgcgt cttgtgccaa
     1441 ccgacttcgt tgccgaattc gaaataaggg ttcccccgct ccttgatgcg gccgacgatc
     1501 tccttctcga tgatcttgac gtgcttcgga ttctgcggat cgaacatctc cggcggcgcg
     1561 tgacgggagg cgtaggcggt gatgccatag tttgtccgga aatggttgtt gtcgccgtag
     1621 cggaggatcg acacgccgag acgttccatc agccgctcgg cttcgggcgt ttcgggatag
     1681 gacgccatcg ccgcgatcga cgtgtcgcga tgcgtgaacc ggtgcggcgg caacacggca
     1741 acattcatcc gcgcgaagcc atcctcgcgc tcgccctccc cgaccgtcga ctcgatgaac
     1801 cagatgccgc gtgcgccctc ttccacggcc ggcaatgcat agtccctgac gaacggcacg
     1861 ccgattttga actcgaacct ctcggactga tcgaaaacga ccttgccgtc gaagtcccgc
     1921 gccaccgtcc gtactgtccg ccagccatcg attagcggtt tgaccgtaag ccgaaacttc
     1981 ggcgcgccgc tctcgaacag gttgaacggg cgctccgtcg caagcgcgat cacaaacggc
     2041 cgcccgtcca cctgtgccgc ggcgcattgc gcatcctcgg agtcgacgcc cgtcacgaac
     2101 tcaagccgtc cgtgatagac gccgttcgtt gacgtgcggg ggaaccccgg acactccgtc
     2161 cgcgcgcccg accagccggt gttccggcac cagaagacct gaccggactt cgcgcgatac
     2221 ggcttaacgt tcatgctctt gacctcgaac ggcaccccgg gatcatacca cccttccgcc
     2281 accgccggct tgcaacgttg ccaatagccg cacttcgtca cttgctggct ccgccccgtg
     2341 tcattcaggc ggaaaacctc gaccatctgt ccgccgctcc tgaagcacgg cgggatttcc
     2401 gagacgaaat ccacggcgaa accatgtccc gtcaggtacg cttccgccga aaacggaaag
     2461 ttcgtgccgc tggcatttgt gatctcgtag gcgatccgca gacggccgtt cgccgcccgc
     2521 tgaaccgact tcggcacagc gcacggcacc gaccacaaga acggaacctt cccgaccgca
     2581 aggatgtcgg ttcccgtctt gtccgcaata accagtcggc cgtcggcaaa gcgcagtgaa
     2641 cacagatcat ccgaaagccg cagcgcgtca ttcgccgaaa gcgtccccgc agcccagata
     2701 accgccagca gcatccacct cgtcttcatc gcccggcacc ccctcaacgg aagccgaccg
     2761 gaaaggtcgt cgcgccgaag gtcacgtctc ccgatagcgt atagcctcct cattcgctgg
     2821 agcccctgca tcaacggatc gtcagcacac tcccgctcgg cggcggtttc gcgtagatcg
     2881 tcttcccctt gtcccagaca agggtccagc tcgggggcag gatggcgctt gcattgagct
     2941 ggtcaacgac ggtttgggac atatccagcc accctcccgc cgtgtagaag agcggcaccg
     3001 gctctgtccc cttgtaagcg gaaccatcaa tttcaaatac ggtattacca taaagcgttg
     3061 ccgttcgatt cgcgacctgg ttctgcatcg gcgcggcagc atagccctgt tcgggaatga
     3121 cgaacttgaa gtgcacggga tccagaagcg gctgtccgct cgccgtaccg aggacccagg
     3181 ccttccaatt cggcgtggag acacaacgca tcagcgggtt tgtgccacgg aattcaatcg
     3241 cggcgttcgg gctgttcgtc cagttgaagg catccacatt gccgctgccg acgcagacgc
     3301 gcccgttcat cgtgtaggtc gcattcgaaa tgaccaatcg gccgttcggc ttctctgcaa
     3361 aggaaatcac gctcccgtca cctgacgtat agtactccgt gccgtccttc aggacaaaaa
     3421 cgttgttcgt gccgccaagg acgaccgcat tctggccggc gttgttcgtc caggcggcct
     3481 ccagttcaca aaggcagtcc ttagaagctg caccgaatgt caacaccccg ttcgcacgct
     3541 tcgccccgat ggaaaagtat ccgttcgtgc cgctgaaggt caggctcttg tccatcgtgc
     3601 cgcccacgaa ctcgtagcga ttggaaaagc ccccgatggt cacgccgtcg ggcgccgagc
     3661 aggtaccgcc cgtctgacgg atcaggttcg gcccatttcc tcccattgtc aatgttccct
     3721 tgtttcccgt caccaacaga ccgtccgaca ggagcaaccg attgtaccgg ccattgacct
     3781 ggcagcttcc cgacgacagc tcgcccccca gcacttcaac ccggttcgag acgccattca
     3841 tcaccaccgg aggcgaggcc ttggccgtgt cctgaatccg gagaaggtcc caggcgccgt
     3901 tcatgtcaaa ggtcgagaag ccgctggcaa cacttgaccc ggaaacgacc atctcattgg
     3961 aaaccgaata cccggaccgc cattcaaaat accgccgccc cgtatattgg ggtgtcgcct
     4021 gcggcacatt catgtaaaag gtccacgtta gccattagct attgcaacac ctttaacccg
     4081 ctgaacgtca acaagta
//
LOCUS       DAOSRB010000241         4058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_19908_length_4058_cov_8.728703, whole genome shotgun sequence.
ACCESSION   DAOSRB010000241 DAOSRB010000000
VERSION     DAOSRB010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19908_length_4058_cov_8.728703"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLZ74_10175"
     CDS             <1..1190
                     /locus_tag="PLZ74_10175"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOR98724.1"
                     /translation="ELVDEHYYKPPEWFLAQATRYDCYDRIGPKVYVGEYACHTHDRK
                     NNLLAALCEAAAMTGFERNSDVVCMAAYAPLFNKIGSRQWDIDLIWFDNTRVFGTPSY
                     YVQKLFMNNLPDVLLPVEVSNHLVTLPPTGTIGLHTWETAAEFKDIRVTRGNETLYAF
                     DPQTGTKGWSKSKKGVWSVKDGVLRQSDAAVRETVTSFIAGASWENYTLELNARKLSG
                     KEGFIIRVRDQRDKMIHLNLGGWGNTEHGIEQNGQNPIIRQPGSIETGRWYAIKIQLE
                     GERVRAWLDGQPLFDRLLPNRQAARAYLVAGHDKTAGEIVVKGVNPRNAPVTLALKLA
                     GADVQAQKARRITLTGRPDDVNTMEEPCKITPQEDTLDIPGAGSQVILPAYSLTILRI
                     KAG"
     gene            1407..2639
                     /locus_tag="PLZ74_10180"
     CDS             1407..2639
                     /locus_tag="PLZ74_10180"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOR98725.1"
                     /translation="MAKQVKKVGFLTAGGLAPCLSSAIGYLIDEYNRVAPDVELIGYV
                     NGYKGLLRGESLPVTPEVRRHALILTNHGGSPIGNSRVKLTNVKDCVKRGLVKEGENP
                     QKVAADQLVRDGVDVLHTIGGDDTNTAAAELAAFLAQNGYGLTVVGLPKTIDNDVYPI
                     RQSLGAWTAAEEGARFFEHIVKENTANPRMLIIHEVMGRNCGWLTAATAAAYRKRLDS
                     FSYLPEFGLSRDREEVHAVYIPEMTFDVRVEAERLRKVMDRNDGVNLFVSEGACVKTI
                     IAELEAKGEEVPRDAFGHPRLDKVNVGGWFAKQFGEMLGAEKTMVQKSGYYSRAAAPN
                     DNDKELIRACAKKAVECALAGIGGVVGEDEDRNGELRACEFPRIRGGKPFDIETPWFT
                     RMLEEIGQPRGTPIVVQH"
     gene            2697..3425
                     /locus_tag="PLZ74_10185"
     CDS             2697..3425
                     /locus_tag="PLZ74_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOR98726.1"
                     /translation="MAEERALSEILAEVQDKIIAACARSGRDAETVEVIGVTKTHGPE
                     VVREAWEAGIRLFGENRVQEAAWKIPACCGGPVWHMIGHLQRNKVRAALEWFRVIHSV
                     DSLRLLEQIERVAEESGHRPEILIEVNVAGEASKDGARPEAVPELIEAALRARNVTLT
                     GLMTMAPFVTDPELTRPVFARLRTWRDRWEREFGIGLPQLSMGMSNDYPVAIEEGATW
                     VRLGTALFGSRPKWRPQIEERWEP"
     gene            3553..3942
                     /locus_tag="PLZ74_10190"
     CDS             3553..3942
                     /locus_tag="PLZ74_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98727.1"
                     /translation="MKPDTKEQLNLKRAVEPSTLFGFMVSWFRGFWFHGFMVSRFQGF
                     IQIGIGIGIAIGIDCFTTEHFPGFQGFGVSGFHGFIQIGIGIAIGIGIDHLTTEHFLG
                     FHGFKVSGFMVSSKSGSGSLSGSGSII"
BASE COUNT          842 a         1048 c         1360 g          808 t
ORIGIN      
        1 ccgaactggt ggacgagcac tactacaagc cgccggagtg gtttctggct caggcgacgc
       61 gctatgactg ttatgaccgc atcggcccga aggtctacgt cggcgaatac gcctgtcata
      121 cgcacgaccg caagaataac ctgctcgccg cgctgtgcga agcggctgcg atgaccggct
      181 tcgagcgcaa ctcggatgtg gtgtgcatgg ccgcttatgc gccgctcttc aataaaatcg
      241 gcagcaggca atgggatatt gacctcattt ggttcgataa cacacgcgtc ttcggtacgc
      301 cctcgtatta tgtgcagaag ttgtttatga acaacctgcc ggatgtgcta ttgcctgtgg
      361 aagtatcgaa ccatctcgtg acgctgccgc cgacggggac gatcgggttg cacacatggg
      421 aaacggctgc tgaatttaag gacatccgcg tcacgcgcgg taacgagacc ctctatgcct
      481 ttgacccgca gacgggtacg aaaggctggt cgaagtccaa gaagggcgtg tggtcggtca
      541 aggacggtgt gttgcgtcaa tccgatgcgg cggtgcggga gaccgttacc agcttcattg
      601 cgggcgcgtc gtgggagaac tatacgctcg aactcaacgc ccgcaagctg tcgggcaagg
      661 agggctttat catccgtgtc cgcgaccaac gcgataagat gattcacctg aacctcggtg
      721 gctggggcaa cacggaacac ggcatcgagc agaacgggca gaatccgatc atccgccagc
      781 ccggctcgat tgaaaccgga cgctggtatg cgatcaagat tcagcttgag ggtgaacgtg
      841 tccgcgcttg gcttgacggc cagccgctct ttgaccggct gctgccgaac agacaggcgg
      901 cgcgcgccta tctcgtggcc ggtcatgaca agacggcagg cgagatcgtg gtgaagggcg
      961 tcaatccgcg caacgcgccg gtgacgctcg cgctcaaatt ggcgggcgcg gatgtgcagg
     1021 cgcagaaggc gcggcgcatc acgttgaccg gtcggccgga cgatgtgaac acgatggaag
     1081 agccgtgcaa gatcacgccg caagaagaca cgcttgatat tccgggcgca ggttcgcagg
     1141 tgatccttcc cgcgtattcg ctcacgattc ttagaatcaa ggcagggtag aggacagaat
     1201 gaaagggaag gcgcactttc gttgtgtttg ttagttcgta aggcactaac gtcggcggag
     1261 agacggcccc ggccggaacc gaggtctccc gccttgcagg gcgcgttcgc tgaaaaggat
     1321 ctgaccggac gggtcttgaa atagaggccg cgttctttta cactaaccgg gtttttatgg
     1381 caatccgtat gggagaagga gaagacatgg cgaaacaggt gaagaaggtg ggttttttga
     1441 cagcgggcgg tttggcgccc tgtctgagtt ctgcgatcgg atatttgatt gacgagtata
     1501 atcgggtggc cccggatgtg gagttgatcg ggtacgtgaa tggatacaag gggttgctgc
     1561 ggggggagag tctgccggtg acaccggaag tccggcgtca tgcgctgatc ctgaccaatc
     1621 acggcggcag cccgatcggc aacagccgcg tgaagctcac gaatgtgaaa gactgcgtga
     1681 aacgggggtt ggtgaaagaa ggggagaacc cgcagaaggt cgcggcggac cagttggtgc
     1741 gggacggcgt ggacgtgttg cacacgatcg gcggggacga taccaacacg gccgccgccg
     1801 agctggccgc gtttctggct cagaacggat atgggctcac ggttgtgggg ttgcccaaaa
     1861 ccattgataa cgacgtgtac ccgatccgtc agagtctggg ggcctggact gcggcggaag
     1921 aaggcgcccg gtttttcgag cacatcgtca aggaaaacac ggccaacccc cgcatgctga
     1981 tcatccatga ggttatgggg cggaactgcg ggtggctgac cgccgcgacg gctgcggcgt
     2041 atcgcaagag actggacagt ttttcctatc tgccggagtt cgggctgtcg cgtgaccgcg
     2101 aagaggtcca cgctgtgtac attcccgaga tgacatttga cgtgcgtgtc gaggcggaac
     2161 ggttgcgcaa ggtgatggac cgcaatgacg gcgtcaacct cttcgtttcc gagggggcct
     2221 gcgtcaagac gatcatcgcg gagttggagg cgaagggtga ggaggtgccg cgtgacgcgt
     2281 tcggccaccc gcgactggac aaggtgaatg tgggcggctg gttcgccaaa cagttcggcg
     2341 agatgttggg cgcggaaaaa accatggtgc agaagagcgg gtattacagc cgcgccgccg
     2401 cgccgaacga caacgacaaa gagttgatcc gcgcctgcgc gaaaaaggcg gtcgagtgcg
     2461 cgctcgccgg aatcggcgga gtggtggggg aagatgaaga ccggaatggc gagcttcgcg
     2521 cctgcgagtt cccgcgcatc cgtggcggta agccgttcga tatcgaaacg ccgtggttca
     2581 cccggatgct ggaggagatc ggccagcccc ggggtacccc gattgtcgtg caacattgaa
     2641 cggggttcgg aggcggtgcc cgaaaccggg catcgctccg cgacagaaag agggatatgg
     2701 cggaggagcg cgcgttatcc gaaatcctcg cagaggtgca ggataaaatt atagcggcat
     2761 gtgcccgatc tggccgcgac gcagagaccg tcgaggtgat cggggtgacc aaaactcacg
     2821 gaccggaggt ggtgcgcgag gcctgggagg ctggaatccg cctgttcggc gagaacaggg
     2881 tacaggaggc ggcctggaaa atcccggcat gttgcggcgg tccggtctgg catatgatcg
     2941 gccacctgca gcgcaacaag gtgcgcgccg cactggaatg gttccgagtg attcattcgg
     3001 tcgactccct gcgcctgctg gagcagatcg agcgggtggc ggaagagagc gggcaccgtc
     3061 ccgaaattct gatcgaggtc aatgtggcag gcgaggcgag caaggacggg gcgcgtcccg
     3121 aggcggtgcc cgaactgatc gaggctgcgc tacgcgcgcg taatgtgacc ctgacgggct
     3181 tgatgacgat ggccccgttc gtaaccgacc cggagctgac gcggccggtt ttcgcgcggt
     3241 tgcgcacgtg gcgcgaccgg tgggagcgcg agttcggaat cggcctgccg caactgtcga
     3301 tgggcatgag caacgattac ccggtcgcaa tcgaggaggg ggccacctgg gtgcggctcg
     3361 gcacggcgct gttcggctcg cggccgaaat ggcggcccca gatcgaagag cgctgggagc
     3421 cctgacaaag ggagggatgc ccgtctgctc gtccgggcaa gcagagtttc gcacaccaaa
     3481 acgtattgcc cagtgcctct tgttttggct ttcgttgaga tccgcaccgc acctatttgg
     3541 tgcaaatgaa acttgaagcc agacaccaaa gagcaactga acctaaaaag agcagttgaa
     3601 ccttcaactc tctttggttt catggtttca tggtttcggg gtttctggtt tcatggtttc
     3661 atggtttcac ggtttcaggg tttcatccaa atcggtatcg gtatcggtat cgctatcggg
     3721 atcgactgtt tcaccacaga acactttcct gggtttcagg gtttcggggt ttcagggttt
     3781 catggtttca tccaaatcgg gatcgggatc gctatcggga tcgggatcga tcatttaacc
     3841 acggaacact ttcttgggtt tcatggtttc aaggtttctg gtttcatggt ttcatccaaa
     3901 tcggggtcgg gatcgctatc gggatcggga tcgatcattt aaccacggaa cactttcttg
     3961 ggtttcatgg tttcaaggtt tctggtttca tggtttcatc caaatcgggg tcgggatcgc
     4021 tatcgggatc gggatcgatc atttaaccac ggaacacc
//
LOCUS       DAOSRB010000242         4006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_20245_length_4006_cov_14.171602, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000242 DAOSRB010000000
VERSION     DAOSRB010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20245_length_4006_cov_14.171602"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLZ74_10195"
     CDS             complement(<1..221)
                     /locus_tag="PLZ74_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98728.1"
                     /translation="MSAAMWIPVATVAASRFPQPQFDSGHTVPQTVHPPFAALVPAWA
                     DVLILALALAVAVWLAHRVRSRRWLLVFSL"
     gene            complement(224..784)
                     /locus_tag="PLZ74_10200"
     CDS             complement(224..784)
                     /locus_tag="PLZ74_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR98729.1"
                     /translation="MREREQEATRRDALAWLLRAGAGAVVASFAAKAAQRQTADRTVW
                     QIDPRACIQCGRCETECVLHPSAVKCVHGYAMCGYCKLCFGYFKSDAARLDEAAENQI
                     CPTGAISRVFIEDPYFEYHIDESLCTGCGLCVKGCTHFGNGSLYLQIRHDRCTNCNAC
                     RIAENCPAQAISRVPASRPYLLKESL"
     gene            complement(815..3067)
                     /locus_tag="PLZ74_10205"
     CDS             complement(815..3067)
                     /locus_tag="PLZ74_10205"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR98730.1"
                     /translation="MNVFKVIGLAGIVVPGLLMAQTWTYTPPAYPGVCTNPAVVGEWT
                     MDYETAVQRAAAEGRNLYLLFTGSTWCPDCDTLQNQVMSTPAMQTFMRESDAYWVWLD
                     FPSRRATNAVERGWLCHTNTGLFTLEQSEAILARNRQLEYIYGSIKNYRAAPGTLNMP
                     TFVICHPDGSYQGEVTHYRTWTYVTPDLFLWKIRRIWNDDPWDVQDNGVPGLSDDAPE
                     TATPITDMSADKQVQARTLSPTDGADWFAFTAVPGFTYAFALEGQLLGGQGDLPAGLI
                     GLDVYAGTDLTEAVASSSGLLGRRQIVTWTFDGTEPCAVSLRVSGSFGEVTGYTLSYR
                     QVAVAAPTARAIRRTEGVFYDEAGTARVAGLISLSVSRSGFLSAKYRSLDATVSFSAR
                     KFWNGVDKNGAVLSSISKGAYRLDVRLPGTGDIFMTLTDPAFEAPLNAVLREVTDVDP
                     HWADAYQGYYTMVLEPMLTEGPSAPMGYSFLTCRVRSSAARSGKTNYSGRFADGKSFS
                     GSAVFRLGDSCSAEMIVLLSNRRYRMAGALSICADAAEVRWTDPSVVTVVHGTQMFWA
                     HTTGSPGTSFDITVSLAGGYYDMRDSLTDYLTLYPGEGAVCLLGPSMLPFSEFYGFAQ
                     QQLDLTMDVTPTTMRLAKGQANPMRARFSLNKRTGIFSGSFRMPMGSATGAITTVPVR
                     FAGVLLPGWIGGSGGCDDGCGPPTQNTTPYKPFGMGTLWFKDKATSEQTGRPIAVTAS
                     YPLTIEELAN"
     gene            complement(3117..3764)
                     /locus_tag="PLZ74_10210"
     CDS             complement(3117..3764)
                     /locus_tag="PLZ74_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOR98731.1"
                     /translation="MEQIIQWVVNTVGQWGYAGIVIMMFLESSCFPFPSEVVMIPAGY
                     LSSPNYIHSEGYVAGHEMNVWVAIAMGIIGSWLGALFNYYLALLLGRPLLLKWGKYFF
                     ISEENFEKAERFFKRHGEIGTFTARLIPVVRQYVSLPAGLVRMNMARFLFYTGLGAGL
                     WVSILAWIGYIAGQSEALWRPYLKRATVAALVFCVVVVAVYVLVRRARGKKSDAA"
BASE COUNT          849 a         1319 c         1151 g          687 t
ORIGIN      
        1 agagagaaca ccagcagcca acggcgtgaa cgcacccgat gcgccagcca caccgccacc
       61 gccagcgcaa gcgccaagat cagcacatcg gcccaagcgg ggaccagcgc cgcaaagggc
      121 ggatgcaccg tctgcggcac ggtatgcccc gaatcaaatt ggggctgcgg aaaacgtgac
      181 gccgcgaccg tggccacggg aatccacata gcggcggaca tggctaaaga ctctctttca
      241 gcagataggg acgggaggcc ggcacgcggg agatcgcctg cgcggggcag ttctcggcga
      301 tgcggcaggc gttgcagttg gtgcaacgat catgccggat ctggagatag agcgacccgt
      361 tgccgaagtg ggtgcagccc ttgacgcaaa gcccgcatcc cgtgcagagc gactcgtcga
      421 tgtgatactc gaagtaggga tcttcgataa agacgcggga gatcgcaccg gtcggacaga
      481 tctggttttc agcggcctcg tcaagacgcg cggcatcgga cttgaaataa ccgaagcaga
      541 gcttgcagta accgcacatc gcgtaaccgt gcacacattt aaccgccgat gggtgcagca
      601 cgcactcggt ttcgcaacgg ccacactgga tgcatgcgcg cggatcgatc tgccataccg
      661 tgcggtccgc ggtttgccgc tgcgcagctt tggccgcgaa cgacgccacc acggcgcccg
      721 cgccggcccg caacaaccaa gccagcgcat cccgacgcgt tgcctcctgt tcccgttcgc
      781 gcatgcatcc cgcccttctc cgcgcaaggc ggcgtcagtt ggccagttcc tcgatcgtca
      841 gcggatagct cgccgtcacc gcgatcggcc gaccggtctg ttccgaggtg gccttgtcct
      901 tgaaccagag ggtgcccatg ccgaacggct tgtagggcgt cgtgttttgc gtgggcggac
      961 cgcatccgtc gtcgcatccg ccgctcccgc cgatccagcc cggcaacagc acgccggcga
     1021 acctgacagg aacagtcgtg atcgcaccgg tcgcggatcc cataggcatc cggaacgagc
     1081 cggagaaaat ccccgtccgc ttgttcagcg agaagcgggc ccgcatggga ttggcctgac
     1141 ccttcgcgag tctcatcgtc gtcggcgtca catccatagt cagatcgagc tgctgctgcg
     1201 caaacccgta aaactcgctg aacggtaaca tgctggggcc gagaaggcat accgcgcctt
     1261 caccggggta cagcgtcagg taatcggtca gcgaatcgcg catgtcatag taaccgcctg
     1321 ccagcgacac cgtgatatcg aatgaggtgc ccgggctccc cgtggtatgg gcccagaaca
     1381 tctgcgtgcc gtgaaccacc gtgaccacgc tcggatcggt ccagcgcacc tccgcagcat
     1441 ccgcgcagat cgaaagcgcg ccggccatgc gataacgacg gttactcaac agaacgatca
     1501 tctctgcgga gcaggagtcg cccagccgga aaacggcact gccgctaaag gattttccat
     1561 ccgcaaagcg tcccgagtag ttggttttcc cggagcgagc ggccgacgac ctgacccggc
     1621 aggtcaggaa cgagtacccc atcggggcac tcggaccttc cgtcagcatg ggctccaaga
     1681 ccatcgtgta atatccttgg taagcgtctg cccagtgggg gtccacatcc gtgacctcgc
     1741 gcagcacggc gttcagcggc gcttcgaagg ccggatcggt cagcgtcata aaaatgtcac
     1801 ccgtccccgg cagccggaca tccagacggt aagccccttt cgagatcgaa ctcaggaccg
     1861 cgccgttctt gtccacgccg ttccagaatt tccgggcgct gaacgagaca gtcgcatcaa
     1921 gcgaccgata cttggccgac agaaatccgg agcgcgagac cgacagtgaa atcaatcccg
     1981 ccacgcgggc cgtgccggcc tcatcgtaaa acaccccttc cgtacgccgt atagcgcgag
     2041 ccgtaggcgc ggcaacggcg acctgacggt aggagagcgt gtagccggtt acctcgccga
     2101 acgaaccgct cacgcggagc gacaccgcgc acggctccgt cccatcgaac gtccatgtca
     2161 caatctgccg ccgccccaac aggccggaag aggaggcgac agcctcggtc aggtccgtgc
     2221 cggcatacac atcgagccct atcaggccgg cgggcagatc accctgtcca cccaacagct
     2281 ggccttccaa cgcgaacgca tacgtgaagc cgggcacggc agtgaatgcg aaccaatccg
     2341 cgccgtcggt cggtgaaagc gtacgcgcct gtacctgttt atcggcagac atgtccgtga
     2401 tcggggtcgc ggtttccggc gcgtcatcgc tcagaccggg cacgccattg tcctgcacat
     2461 cccacggatc atcgttccaa atgcgccgga ttttccacag gaagaggtcg ggcgtcacat
     2521 acgtccaagt gcggtaatgc gtgacctcac cctgatagct cccgtccggg tgacagatca
     2581 caaacgtcgg catgttcagc gttccagggg cagcccgata atttttgatc gagccgtaga
     2641 tgtactccaa ctggcggttc cgggcgagga tcgcttccga ctgttccagc gtgaaaagcc
     2701 ccgtgtttgt gtggcagagc cagcctcttt ccactgcgtt ggtcgcgcgg cggctgggga
     2761 agtccagcca aacccagtag gcgtcgctct ctcgcatgaa cgtctgcatg gccggcgtcg
     2821 acatgacctg attctgcagg gtatcgcaat ccggacacca agtggagccc gtgaacagca
     2881 ggtacagatt ccgtccctcg gccgccgccc gttgcacggc cgtttcgtaa tccatcgtcc
     2941 attcgccgac aaccgccgga ttggtacaga caccgggata cgccggcggg gtataggtcc
     3001 acgtctgcgc catcaacagt cccggcacaa cgatccccgc aagtccgatc actttgaaca
     3061 cattcatcgt catccctcct caatggcgtt gccgtgcata cacggccccg ccggtctcac
     3121 gccgcatcgc tttttttgcc acgcgcgcgg cgcacaagaa catacaccgc cacgacgacc
     3181 acgcagaaca ccaaggccgc gacagtcgcc ctcttgaggt acggccgcca cagcgcttca
     3241 ctctgaccag cgatgtaacc gatccaggcc aagattgaaa cccagagccc ggcaccgagt
     3301 ccagtataga agagaaagcg cgccatgttc atccgcacga gacccgccgg cagcgacaca
     3361 tactgccgca caactggaat caggcgcgcg gtgaaggtac cgatctcgcc gtgccgtttg
     3421 aaaaaacgct cggctttctc aaaattctcc tcggaaatga aaaagtattt gccccacttc
     3481 aacaggaggg ggcgccccag cagcaacgcg agatagtagt tgaacagcgc tcccagccaa
     3541 ctgccgatga tccccatcgc aatggccacc cagacattca tctcatggcc cgccacataa
     3601 ccctcggagt gtatgtagtt gggcgacgag aggtagcccg ccgggatcat gacaacctcg
     3661 ctggggaacg ggaaacagga actctcaaga aacatcatga tcacgatgcc cgcatagccc
     3721 cactgcccca ccgtgttcac tacccactga atgatctgct ccatgccacc tccgattggg
     3781 gtgggggcaa gtgcttccgc gcgttgcgcg gggggaaagc ggtgccgcga cccgcgcaca
     3841 gcgcggctct tgtcaccgca ctccaaatct tatgcactta cttgagtgtt ttccggaacc
     3901 gccaagatgc ccccgtttcc catgaagttt catgtgtttc atggttccat cttaaatggt
     3961 cggggcgtcg ggatttgaac ccgagacctt ttgcacccca agcaaa
//
LOCUS       DAOSRB010000243         3960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_20555_length_3960_cov_6.573624, whole genome shotgun sequence.
ACCESSION   DAOSRB010000243 DAOSRB010000000
VERSION     DAOSRB010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20555_length_3960_cov_6.573624"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLZ74_10215"
     CDS             <1..734
                     /locus_tag="PLZ74_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR98732.1"
                     /translation="SYAARRGEGPAYETALAAIAACRRQGLVTGVATSVSASNFRDVV
                     NPAFLDDMVARGAHYVWYYLYRPSGPEPGFGEALNDTQVREARRFLLEQRRRCRTAVI
                     IDAYWDHEGNALCPAATGISHHINARGDVEPCPPIQCADSRVRPDAPVAATIRDSPLL
                     AAFRSEVPRLTRGCVLMDHPAELAALARRHQAYDSSGRGCFFEELAARPILGCHNHSG
                     EPLPETSWAYRFAKKHWFFGFGAYG"
     gene            complement(748..3552)
                     /locus_tag="PLZ74_10220"
     CDS             complement(748..3552)
                     /locus_tag="PLZ74_10220"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98733.1"
                     /translation="MCFAKRLGRLLCCVEGVFIVCGMALGAEIHVSPKGSDEAPGSVR
                     EPVLTLEKARLLAREARARQPGEPVSLLLHPGTHRVTRTVAFTAEDAGTREAPLQLLA
                     WRTPSTPPEARPVLVGGKVVTGWKACSFNGRDGVFEADLKPLGITTPFRQVYLNGQRL
                     IWARYPNADPALPYSGGWAYVDGVRPPMYKDIEGERTDTVVLRERDVHAWSRPEEGEM
                     CIFPRYNWWNRIEKIAAFDPGSRTFTLAKKMPYAARPEDRYCVMGLREELDAPGEWFQ
                     DVAGQRLYLIPPAGADLTRDQITVPTVNDILSFNKTHHVRIANLELTCAETHALRFQD
                     CDHMTVEACRIHDLGFFSGSGIGIRGGREGRIRGCDIWHIGGHGVSVYAGDVDSMTRC
                     FHEVENCYIHHVGQFNRHGLGVMIGGAGVRVAHNLMHDLPRSGIFHGGVLHTLEYNRI
                     RHCNLEMEDTGCTYTGGWCGGWHTIRYNHCSDSIGFNNHGKFFVFAWGIYLDESGCGN
                     DVYGNLVERCQVGAMHLHNARENHIYNNIFANNAGPEGKTHQFSLQTWNNSPTAVFLR
                     DRQPKMLKAYTRLMANPEWAKMRGMHVSPADPFLPDGTIMRGNRIFRNIFLYSEQPDS
                     RYIRENGVNLTHNLINSNVVWNGGAQPVKTGKQAVRRPIADLTERIPNAAFARAVDAA
                     ALASDPNQTIAAEWFWYQKILPGLQAERVTRGDAQALRLFGAFNGERKYIKYPCVRSA
                     PFTLPYGKHYRLTFALRHHEADGEVLVRLVCENKGLWKALGPRGFLKRSDMSDISTAS
                     EALPCETGFYLPKPGEAGFDARMEAFTLHFEFQSKQGWAEISGLRLEEVEPASEWEAW
                     QMAGADRDSVVADPQFVDAARGDFRLKPDSPALKLGFEPIAFDKIGPYPDAARATWPI
                     IEAEGVREHPEWLTSVPIPK"
BASE COUNT          753 a         1334 c         1154 g          719 t
ORIGIN      
        1 tctcctatgc agcgcggcgg ggcgagggcc cggcctacga aaccgctctg gcggccatcg
       61 ccgcctgccg tcggcagggt ctcgttaccg gcgtggccac cagtgtctcg gcctcgaatt
      121 tccgtgatgt cgtgaacccc gcgtttctgg acgacatggt ggcgcgcggc gcccattacg
      181 tctggtatta cctctaccgc ccctccggtc cggaacccgg tttcggagag gcgctgaacg
      241 atacccaagt gcgcgaagcg cgccgcttcc tactggagca gcggcggcgc tgccgcacgg
      301 cagtgatcat cgacgcgtat tgggatcacg agggcaacgc gctctgcccg gccgcgaccg
      361 gcatcagcca ccacatcaac gcgcgcggcg atgtggagcc ctgcccgccg atccagtgcg
      421 ccgacagccg cgtgcggcct gacgcaccgg tcgcagcgac gatccgcgac tctcccctgc
      481 tggccgcgtt ccgcagcgag gtgccgcgcc tgacgcgcgg ctgcgtgctg atggatcatc
      541 cggcggaact cgccgcgttg gcgcggcgtc atcaggcata cgatagcagc ggtcgcggat
      601 gtttcttcga ggaactggcg gcacgaccga tcctcggctg ccacaaccat agcggcgaac
      661 ccctgcccga aacctcttgg gcctaccgct tcgccaagaa acactggttc ttcggcttcg
      721 gagcgtacgg ctgaaacagt gccggcctca tttcgggatc ggtaccgacg tgagccactc
      781 cggatgctcg cgcacgcctt ccgcctcgat gatcggccaa gtcgcacgcg ccgcatccgg
      841 gtacgggccg attttgtcaa acgcgatcgg ctcaaaaccc agtttcaggg cgggcgaatc
      901 cggtttgagc cggaagtcgc cgcgcgccgc gtcaacaaac tgcggatcag cgaccacaga
      961 atcccggtcg gcacccgcca tctgccatgc ctcccactcg ctcgccggct caacctcctc
     1021 gagccgcaga ccgctgatct cggcccatcc ctgtttggat tggaactcga agtggagcgt
     1081 gaacgcttcc atacgcgcat cgaacccggc ctctccgggt ttcggcagat agaatccggt
     1141 ttcgcagggt agcgcctcgg aggcagtgct gatatccgac atgtcagaac gtttgaggaa
     1201 cccccgcgga cccaaggctt tccacaaccc cttgttctcg cagaccagac gcacgagcac
     1261 ctcgccgtcc gcctcgtgat gacgcagcgc aaacgtcagg cggtaatgct tcccgtacgg
     1321 caacgtgaac ggcgcggaac ggacgcaggg gtatttaatg tacttgcgtt ctccattaaa
     1381 ggcaccgaac agacgcaacg cctgagcatc cccacgcgtc actcgttccg cctgcagccc
     1441 cggcaggatc ttctggtacc agaaccactc cgccgcaatc gtctggttcg gatccgacgc
     1501 caacgcggcc gcgtccaccg cacgcgcgaa tgcggcattg gggatgcgct ccgtcaggtc
     1561 cgcgattggc ctccggacgg cctgtttgcc ggtcttcacc ggctgggcac cgccattcca
     1621 caccacattc gaattgatga ggttgtgcgt gagattcacc ccgttttcgc ggatatagcg
     1681 ggagtccggc tgctcggaat agaggaagat attgcggaag atgcggttcc cccgcataat
     1741 cgtgccatcc ggcagaaacg gatcggcggg cgacacatgc atcccgcgca tcttggccca
     1801 ctccggattg gccatcaggc gcgtataagc cttgagcatt ttgggctggc ggtctctcag
     1861 gaagaccgcg gtcggcgagt tgttccaagt ttgcagggag aactggtgcg tcttgccctc
     1921 cgggccggca ttgttggcga aaatgttgtt gtagatgtgg ttctcgcggg cattgtgcag
     1981 atgcatcgcg ccgacctgac agcgctccac gaggttcccg tagacatcat tgccgcagcc
     2041 gctctcgtcc agatagatac cccaggcgaa aacgaaaaac ttgccgtggt tgttgaaccc
     2101 gatactgtcg gagcagtggt tgtagcggat cgtgtgccag cctccgcacc agccgccggt
     2161 gtaagtgcag cccgtgtcct ccatctccag attgcagtga cggatacggt tgtattccag
     2221 tgtgtggagc accccgccgt ggaaaatccc gctccgcggc aggtcgtgca tcaggttgtg
     2281 cgccacacgg accccggcac cgccgatcat cacgccgagg ccgtggcggt tgaactgacc
     2341 gacatgatgg atataacagt tttcgacctc atgaaaacag cgcgtcatcg aatcgacatc
     2401 tcccgcatac acggagacgc cgtgcccgcc gatgtgccag atgtcgcacc cgcgtatccg
     2461 gccttcccga ccgccccgga tcccgatgcc cgaaccgctg aaaaacccga gatcatggat
     2521 gcggcacgcc tcaaccgtca tgtgatcgca atcctgaaac cggagggcgt gcgtctcggc
     2581 gcaggtcagt tccagattcg cgatccggac gtggtgcgtc ttgttgaagg agaggatgtc
     2641 gttgaccgtg ggaaccgtga tctgatcccg ggtgagatcc gcccctgcgg gcgggatcag
     2701 atacagccgc tgtcccgcaa catcctgaaa ccactcgccg ggcgcgtcca actcttcgcg
     2761 cagccccatg acgcaatacc ggtcctcggg ccgtgcggcg tacggcattt tctttgcgag
     2821 cgtgaacgtg cgcgaacccg gatcaaaggc cgcgatcttc tcaatccggt tccaccagtt
     2881 gtagcgcggg aaaatgcaca tttccccctc ctccggacgg gaccaggcgt gcacgtcccg
     2941 ctcgcgcagc accaccgtgt ccgtgcgttc accctcgata tccttgtaca tcgggggccg
     3001 gaccccgtca acgtaagccc acccgccgga gtagggcaaa gccggatccg cattggggta
     3061 acgcgcccag atcagacgct gaccgttgag atatacctga cggaacggcg tggtaatgcc
     3121 gagcggttta agatccgcct cgaacacccc gtcccggccg ttgaaggagc aggccttcca
     3181 gccggtgacc accttgccgc caaccagaac cggccgcgcc tccggcggtg ttgacggtgt
     3241 gcgccacgcc aagagctgta acggcgcctc ccgggtaccc gcgtcctccg ccgtgaacgc
     3301 gaccgtccgg gtcacacggt gcgtgccggg gtgcaacaga aggctcaccg gctcgcccgg
     3361 ctgacgcgcg cgcgcctccc gcgccagcag acgggccttc tccagcgtca gcaccggctc
     3421 gcggacagaa cccggtgcct cgtccgatcc cttgggcgaa acatggatct ccgcgcccag
     3481 cgccattccg cacacgatga agaccccttc aacgcaacac aacaaacgcc ctagccgttt
     3541 cgcaaaacac atacagaaca ctccccctta taacgcagga tgataaaaac caaaggacaa
     3601 cacagcgaca aatagtaccc caaccaccat caaagcgcga gaacagatcg atatgagaaa
     3661 accgcctgcc gagatttggg gtgtatcaag ccatgcggct cacggggacg ctcgccctcc
     3721 caaaaaatct ctgtgtctcc gcgtctctgc gtcatcaaga agcgcaatgc tatttgtggc
     3781 cggtttagta aggacacagg cagtgtggca caccgtgtcg tccccgtcaa cctatacctc
     3841 ccagcccttg cggtagctgc gcttcaggaa tgcgttcgcg ctctccttgt tggtgaagcg
     3901 gccaaccttg gcgtcccact tgagttcatc gccgctgtct tccatgatcg ccgtcacgcc
//
LOCUS       DAOSRB010000244         3947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_20642_length_3947_cov_6.854317, whole genome shotgun sequence.
ACCESSION   DAOSRB010000244 DAOSRB010000000
VERSION     DAOSRB010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20642_length_3947_cov_6.854317"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1274)
                     /locus_tag="PLZ74_10225"
     CDS             complement(135..1274)
                     /locus_tag="PLZ74_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98734.1"
                     /translation="MFGKLCATVSAGVMIGCCAGTALAQGSLTPPPGAPGPSMKTLAQ
                     VEPRTPIMNVPYTITQPGSYYLTANLVTTSSGIIISTNDVTVDLMGFSINGDRGTYDY
                     GVFVDGKTNAPCRNIVVRNGTIKNFGNGLGLVNAQHSLFEYLMISGSVSTGILLDGTS
                     GGDCSGNRIANCRVSDGCCGIVLAGAWYGDSAGKCDGNSISDCTFRKNAQEGVYIRVG
                     SGQCNHNEFRNCMIQDNASGGIYAYIANGCYCGNVIERCNISGNNSYGIECTCYEENN
                     TAVGNRISQCTLYGNKSAGIRLYGSAFQVDGNLVSGTQGTSTHGIFISTSWPSLAVQN
                     TCIGHTYNFSVDVTTVTHGPIVTAKGVLSTTNGAAGLSPWANFSR"
     gene            1451..1612
                     /locus_tag="PLZ74_10230"
     CDS             1451..1612
                     /locus_tag="PLZ74_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98735.1"
                     /translation="MRRSYVSGFHYGAIAIGFGTAATFTLVIAGRFVGATVDSDSDTD
                     TDLEIWMEP"
     gene            2051..>3947
                     /locus_tag="PLZ74_10235"
     CDS             2051..>3947
                     /locus_tag="PLZ74_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOR98736.1"
                     /translation="MKKTVLSACVCWAALTGVAGGNDLIIWDDAGAGDKWDLAYPVGN
                     GRLGAMPFGNFPKEKILINEETIWHRDPAKLMPEEGFTYLETVRRLEASGDYAGADRF
                     FEKQFQPDGTNPDSYQPFGWLEIEYVTVSPLRKRYRELDLTTGIAKNVYTLDNGSVIT
                     QNVFACGRDDVIAVTIAADSPIRLRVRIDGGTVRGREIIKAGAATGQDATRYVGHIRV
                     LSADSIRADEGFLDIAGVGPITLLVAVATDLDRRTAGAKLPEGWQQKAARTLDELDGV
                     SGDALQRRAVNEHRQYFGRVELDLGKTADAILALSTPERLKRIQAGSQDDPDLIETYF
                     QFGRYLLIASSRPGSFPANLQGVWNPHVKAPWGSDFHLNINLQMNYWLAETTNLPETH
                     QPLFDFIRYCLPSGREMARRMGMRGWCMGHATDIWANARVMSSRVYWGGSFFGGQWMV
                     LHLLDHYRFNRDKRVLENNWDILTDSAAFAESWLIPDPATGQLLSRPSSSPENAFRYA
                     AADGVMAKAALSSGTSFDQFMILQVFHDYLEAAEILGRTEEPLVKTIRAVLPKVYRPR
                     VAKDGRLMEWRLPFEEVEPGHRHISHVLGAYPGNQIDLDGDSVMRDAVRRTLEFRLKH
                     GGAHTGWS"
BASE COUNT          824 a         1149 c         1128 g          846 t
ORIGIN      
        1 cgccgtccgc ataggcgttg atccagcccg cgttcgcggc gtaggcatac ggcccagctg
       61 ggtcgatcgt cgtgccggcg cgggcctgcg cgcagcagag tgccaacgca agtatccgaa
      121 gtgtcttcat ggggtcacct cgagaagtta gcccacgggc tcagccccgc cgcgccgttc
      181 gtggtcgaca gcactccttt ggcagtcaca atcggcccat gggttaccgt cgtaacatcg
      241 acactgaaat tatacgtatg cccgatgcag gtgttttgaa cagccaggct tggccatgat
      301 gtggaaataa agatgccatg ggtcgaagtg ccttgggtgc ctgacaccaa attcccgtcg
      361 acctggaaag cgctaccata aagacggatg ccggcagact tgttgccata gagggtgcac
      421 tgtgaaatcc gatttcccac agccgtattg ttctcctcat aacacgtgca ttcgattccg
      481 taggaattgt tgccgcttat gttgcatctt tcaatgacgt ttccacagta acagccgttg
      541 gcaatataag cgtagattcc gccacttgca ttatcttgaa tcatgcagtt tcggaactca
      601 ttgtgattgc actggccgga tccgactcgt atatagactc cttcttgcgc attctttctg
      661 aacgtgcagt cggagatcga gttgccatca catttgcctg cagaatcgcc ataccaagcg
      721 ccggccaaaa cgatgccgca acaaccgtcg ctgaccctgc aatttgcgat ccggttgccg
      781 ctgcaatccc cgccactggt gccatccagc aggattcctg tagacacgga accgctgatc
      841 ataagatatt cgaacaggct gtgttgcgca tttacaagcc caagtccatt gccaaagttt
      901 ttgatcgtcc cattcctgac aacaatgttg cggcaggggg cattcgtttt tccatcaaca
      961 aaaactccgt aatcgtatgt cccccggtca ccgttgattg aaaaccccat cagatccacc
     1021 gtgacatcgt tcgtcgagat gatgataccg gaagatgttg tcacaagatt ggcggttaaa
     1081 taatacgagc ccggctgcgt gattgtgtag ggcacgttca tgatcggggt tcgcggttcc
     1141 acctgcgcca gcgtcttcat cgacgggccg ggggcgccgg ggggcggcgt caggctacct
     1201 tgcgcgaggg cggtccccgc gcaacatccg atcatcaccc ccgcagaaac agtcgcacac
     1261 agtttaccga acatacgccg ctccttccct tgaaaggatt gcatccattc ataaccctga
     1321 gaccgttttc aaagcatcgg cacagacaaa caggtcatgg ccgaacagtg aggacatcac
     1381 cttatcttta gtgtcgcaga aattgggatt gcgcgtcaac accgattcgt aatcggttcg
     1441 tgacagggat gtgcgaagat catacgtttc tgggttccat tacggtgcca tcgctatcgg
     1501 gttcgggacc gccgccacct ttacactcgt cattgcgggc cgcttcgtgg gcgcgacagt
     1561 cgattccgat agcgataccg ataccgattt ggagatttgg atggaaccgt gaaacacatg
     1621 aaaccagaaa ccctgaaacc ttgaaaccag aaaccctgaa accgtgaaac cgtgaaaccc
     1681 tgaaacccaa gaaagtgtcc cgtggttaaa taatcgattc cgatagcgat cccgaccccg
     1741 ataccgattt ggatgaaaac ctgacccgcg cccgtttgcg tacccgtgcg cgatgactcc
     1801 tcttcgtgtt tcgtgtacgg ggacgtgaac cgctccgctg tgcacgcact ctggcgcaaa
     1861 gagcgtcgag gccgccggtc ttccgcattt cccacaggtc ggggatgccg ttgccgtcgc
     1921 tgtccgggcc cacattcagc accgaccgtg tggacgcttg acttttcacc attcccccgc
     1981 atactgaaac ctgatgagag atgtgttttc ggttccgaat ccggttcgga gcatgggata
     2041 gggggtaaca atgaaaaaga cggttctttc cgcctgcgtg tgctgggctg cgctgacggg
     2101 tgtggcgggc ggcaacgact tgatcatctg ggatgacgcc ggagccgggg ataaatggga
     2161 tctcgcgtat ccggtcggca acgggcgcct cggcgccatg ccgttcggta attttccgaa
     2221 ggagaagatc ctgatcaacg aggagacgat ttggcatcgc gatcccgcca aactcatgcc
     2281 cgaggagggg ttcacatatc tggaaaccgt gcggcggctt gaggcatccg gcgattacgc
     2341 gggcgcagac cggtttttcg aaaaacagtt tcagccagac ggcaccaacc cggacagcta
     2401 tcagccgttc ggctggctgg agatcgagta cgttaccgta tcgcccctcc gcaaacggta
     2461 ccgtgaactt gacctgacaa ccggcattgc gaaaaacgtc tatactctgg acaacggttc
     2521 ggtaatcacc caaaacgttt ttgcctgcgg acgggatgac gtgatcgcgg tcacgatcgc
     2581 tgcggacagc ccaatccgac tccgtgtgcg catcgacggc ggaacggtcc ggggcaggga
     2641 gatcatcaaa gcgggtgccg ccaccggaca ggacgccacc cggtacgtcg gacacatccg
     2701 tgtgctgtcg gccgacagca tccgtgccga cgaggggttt ctcgacatcg cgggtgtcgg
     2761 gccgatcacc cttcttgtgg cagtcgccac cgatcttgac cgccgcacgg ccggcgccaa
     2821 actccctgaa ggctggcagc aaaaagcggc ccggactctg gatgaactgg acggcgtttc
     2881 gggggacgcg ttgcagcgga gggccgtgaa tgagcaccgg caatactttg gacgcgtgga
     2941 actggatctc ggcaagaccg ctgatgccat tctggccttg tccaccccgg aacgtctgaa
     3001 gcggatccaa gccggtagtc aggatgaccc ggacctgatc gaaacgtact ttcagttcgg
     3061 ccgttacctg ttgatcgcct cctcgcgtcc cggttctttc cccgccaacc tgcagggtgt
     3121 ttggaatccg cacgtcaaag cgccctgggg ttcggacttc catctgaaca tcaatctaca
     3181 gatgaattat tggcttgcgg aaacgaccaa cctgccggaa acgcaccagc ctctgtttga
     3241 tttcatccgg tattgcctgc cgtccgggcg cgagatggcg cgtcgcatgg gcatgcgagg
     3301 atggtgcatg ggacatgcca ccgatatttg ggcgaacgcg cgggtcatgt cctcgcgcgt
     3361 gtattggggg ggctctttct tcggcgggca gtggatggtc ctgcatctcc tcgatcatta
     3421 ccggttcaac cgggacaagc gcgtgctgga aaataactgg gacatcctga ccgattccgc
     3481 agcgtttgcg gaatcctggc tgattcccga ccccgcgacc gggcaattgc tgtcgcgtcc
     3541 gtcgagttca cccgaaaacg cgttccgcta tgccgcggct gacggcgtga tggccaaggc
     3601 cgcactttcg tccggaacct ccttcgacca gtttatgatc ctacaggttt ttcatgatta
     3661 tctggaggcg gcggagattc tcggccgcac ggaagaaccc ctcgtcaaga cgatccgggc
     3721 ggtgctgccc aaggtctacc gtccgcgtgt cgcgaaggac ggacgcttga tggagtggcg
     3781 gctgcctttt gaagaggtcg agcccggaca ccggcatatc tcgcatgtgc tgggcgcgta
     3841 ccccggcaac cagattgatc tcgacgggga ttcggtcatg cgtgacgcgg tccgccgcac
     3901 gctggaattc cgtttgaagc atggcggcgc gcacaccggc tggagca
//
LOCUS       DAOSRB010000245         3936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_20730_length_3936_cov_5.833033, whole genome shotgun sequence.
ACCESSION   DAOSRB010000245 DAOSRB010000000
VERSION     DAOSRB010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20730_length_3936_cov_5.833033"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..352
                     /locus_tag="PLZ74_10240"
     CDS             83..352
                     /locus_tag="PLZ74_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98737.1"
                     /translation="MWYPVSERRRKACGQHRGQSTRGDVLMEYVLLMLLVVLPLVGVS
                     SKLVSVPERTFMVDEAIDGDRFGVIGDAFVDLFQMVLCGVCLPLP"
     gene            428..715
                     /locus_tag="PLZ74_10245"
     CDS             428..715
                     /locus_tag="PLZ74_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98738.1"
                     /translation="MKKWNRNRTKRILCRLMGDRAGGVMMEYVILAVLIAAAVVVAVA
                     MFGKTLVGMFDTAAKGATADHTGARQAQDATRSEQSQKAQEAAQYHDSMHQ"
     gene            806..1333
                     /locus_tag="PLZ74_10250"
     CDS             806..1333
                     /locus_tag="PLZ74_10250"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOR98739.1"
                     /translation="MMETVCPLLCMAGIAAWAGFLSWSDCRTRRLPNALTLGAAVVTL
                     AFRWAYGGWDCLLDGFAAGAAAGGFMLVPFLLRGAGGGDVKMLFAAGAMVGWSRLLTL
                     LWFTSLAGVILGLVMLALGQVDGARLRHLLRCAVDWRYDRKAGAAGLPPREAAQVRVP
                     FSVAISAGLLVTMAG"
     gene            1447..2013
                     /locus_tag="PLZ74_10255"
     CDS             1447..2013
                     /locus_tag="PLZ74_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOR98740.1"
                     /translation="MLEFVMAAPVILSLILACMQFAHIWMAKQVVHYAAYCAARATLV
                     CHKDERDRAAEQAARQVCAWIVRGETAGEPAKRIPGWGEIPGSGGARRKTRLKGGQVE
                     DIGRWNVKATVEHDFALIFPLVGPMIGWALNPWGQNDAWQEQRADDTGNIGDADAVRY
                     PHIRFTETVVLPKPYVTVSKMGLPAPGW"
     gene            2016..3590
                     /locus_tag="PLZ74_10260"
     CDS             2016..3590
                     /locus_tag="PLZ74_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOR98741.1"
                     /translation="MLNAANRTVIRLWKDESGVVFALTVIVFLTLFMMGLGVYAIGET
                     VRQRVELQNAADAAAYSAAITQADGLSRVAAINRAMSWTYVQMVRKEMDYIVDKWLER
                     VLLKWEIDNNLMRAFNAPSPCNNGVPWYVTGYNVSIGNGANHEKVLLNKRHLVTIDQI
                     KQARQNASRDGKDYPTLGGQIDDHKQTIRDMNKKEQKLLEDLPKRIEKTVREVLRANI
                     KDTWNDGFAEGARISFVIEQEKNPAQNYAEILKQDRENDFLRHSNYIPEDGKTAEKVF
                     GEGSEPENWFVETGQKEGIQRAYKQRNRVLLAEWHYTSSLWVPTKSGCRRVATYPRQL
                     REVKAEEVRDSRFTSEIAKANILRKNFFSKKGAIAVGVTRRLNNPLQFLVAGGELGII
                     RPFTVDNGTRHMWTVATARAGYNPPGKGKTQPPADCNGEYEETWEERVDRDDLWNLKT
                     SDWDATLLPLYRAWTQGENRNWDGTSGGKILAQIKEGSWQPLYGEGGSPENERAPKLM
                     GGGNVSYSETDAWVVH"
     gene            3667..>3936
                     /locus_tag="PLZ74_10265"
     CDS             3667..>3936
                     /locus_tag="PLZ74_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98742.1"
                     /translation="MTILTAWTAFAGLGMVFMPSASGADNTGGKTSESEPTAEQVGAG
                     ILHGLTVNLFRLHAASDVDGAVKKWGTGAFQAKWTRDVREVFNEHE"
BASE COUNT          842 a         1111 c         1244 g          739 t
ORIGIN      
        1 gccttgattt tttttatttt gcctcttgcc gaattactga aaccaacgta tactctcatc
       61 aaatgcgatg aggaatgcga tcatgtggta tcccgtgtca gagcgccgtc ggaaggcatg
      121 tggccaacat cgcgggcaga gtacgcgagg cgatgtgctg atggaatacg tgttgctcat
      181 gcttctggtg gttctgccgc tggtcggtgt ctcgtcaaaa ctggtgagcg tgccggaacg
      241 gactttcatg gtcgacgagg cgattgacgg agaccggttc ggtgtaatcg gcgatgcgtt
      301 cgtggatctt tttcagatgg tcttgtgcgg tgtgtgtctg ccactgcctt gacctctgtc
      361 acacaacaac acaacacaac aaaccatgcg gacaaaccga cgggtcaacc cgtcaaagga
      421 gcgggacatg aaaaagtgga atcgaaatcg gaccaaacgc atcctgtgcc gactgatggg
      481 cgaccgggcg ggtggcgtga tgatggagta tgtgatcctc gcggtgctga tcgcggcggc
      541 cgtggtggtc gctgtggcga tgttcggcaa gaccctggtc ggcatgttcg acacggcggc
      601 caagggtgcg accgcggacc acaccggcgc caggcaggct caggacgcga cgcggtccga
      661 gcagagtcag aaggcccaag aggcggccca gtatcacgac agcatgcatc agtaacgcgt
      721 cggaagtccg acccgttatt caaagaaccg ctccgccgcc ggaggctttt ccgcctccgg
      781 atggcggtag cggagggtaa gcgtaatgat ggagacggtc tgcccattac tgtgcatggc
      841 gggaattgcg gcatgggcgg gttttctgtc ttggtcagac tgccgtacgc ggcgtttgcc
      901 gaacgccctc actttgggag cggctgtcgt aaccttggcg ttccgctggg cctacggcgg
      961 atgggactgc ttgttggacg gattcgccgc cggtgcggcg gccggcggct ttatgctggt
     1021 tcccttcctg ttgcggggcg cgggcggtgg tgacgtgaaa atgctttttg cggctggagc
     1081 catggtcggc tggtccaggc tcctgacctt gttgtggttc acctccctgg cgggggtgat
     1141 cctcggctta gtgatgttgg cgctggggca agtggatggc gcacggttgc ggcacctgtt
     1201 gcgatgcgcg gtagattggc gttatgaccg taaggccggt gccgcaggtt tgcctcccag
     1261 agaggcggca caggtccgcg tgcccttcag tgtggcgatc tctgccggct tgctggttac
     1321 gatggcaggg tgaagagtgt gatcccgggc gtattaagac ggagatctga aatgtttagc
     1381 ggagagaccc aaagcccctt gcaggcttgc ccacagaaca gatcgacccg ttcctgcgga
     1441 tcggtgatgc tggagtttgt catggctgcg cccgtgatcc tctccctgat tctggcctgc
     1501 atgcagttcg cccacatctg gatggccaag caggtggtcc actacgccgc ctattgcgct
     1561 gcccgcgcca cgctggtctg ccacaaagac gagcgggacc gggccgcaga gcaggccgcg
     1621 cgccaggtct gcgcgtggat cgttcgcggc gaaacggctg gcgaaccggc caagcgcatc
     1681 ccgggctggg gcgagattcc gggttcgggc ggagcaaggc gcaaaaccag actgaaaggc
     1741 gggcaggtcg aggacatcgg acgctggaac gtcaaagcca cggtggaaca tgatttcgcg
     1801 ttgatctttc ccctcgtcgg cccgatgatc ggctgggcgc tcaacccgtg gggccaaaat
     1861 gacgcatggc aggagcagcg ggctgacgac acaggcaaca tcggcgatgc ggatgcggtg
     1921 cggtaccccc atatccggtt taccgaaacc gtcgtcttgc ccaaacccta tgtgactgta
     1981 tccaaaatgg gcctcccggc ccctgggtgg taatgatgct gaacgcggca aacagaaccg
     2041 tcatccggtt gtggaaagac gagagcggag tggtctttgc cctcacggtc atcgtctttc
     2101 tgacgctgtt catgatgggt ctgggtgtct acgccatcgg cgaaaccgtg cggcagcgcg
     2161 tggaacttca gaacgccgcc gatgccgcag cctactcggc cgcgatcaca caggctgacg
     2221 gcctcagccg cgtggccgct atcaaccgcg ccatgtcctg gacctatgtg cagatggtcc
     2281 gtaaagaaat ggattacatc gttgacaaat ggctggagcg cgtcctcctg aaatgggaga
     2341 tcgacaacaa cctgatgcgc gccttcaatg cgcccagtcc ctgcaataac ggagtcccct
     2401 ggtatgtcac cggctacaac gtctcgatcg ggaacggcgc gaaccatgag aaggtcctgt
     2461 tgaacaaacg gcacctggtg accattgatc agatcaaaca ggcgcgccag aacgccagcc
     2521 gtgacggcaa agactacccc accttgggcg gccagatcga tgaccataaa cagacgatcc
     2581 gtgacatgaa caagaaggag cagaagctac tcgaagatct gccgaaacga atcgagaaaa
     2641 ccgtgcgcga agtgttgcgg gccaatatca aagacacctg gaatgacggc ttcgcggaag
     2701 gcgcccgaat cagctttgtg atcgagcagg agaaaaatcc cgcccaaaat tatgccgaga
     2761 tcctcaagca ggaccgggaa aatgattttc tgcgccactc gaattacatt cccgaagacg
     2821 ggaagactgc ggagaaggtg ttcggcgaag gctcggagcc ggaaaactgg tttgtcgaga
     2881 ccggccagaa ggaagggatc cagcgcgctt acaagcaacg taatcgcgta ctgctcgcgg
     2941 aatggcacta caccagctcg ctgtgggttc cgaccaagtc ggggtgccgc cgggtcgcca
     3001 cctatcccag acagttacgc gaggtcaaag ccgaagaggt gcgggacagc cgcttcacct
     3061 cggaaatcgc aaaagccaac atcctgcgta agaatttctt ttcgaaaaag ggagcgattg
     3121 cggtgggggt gacccgacgc ctgaacaatc cgcttcagtt ccttgtggcg ggcggcgaac
     3181 ttggcatcat ccgccccttt accgtcgaca acggcacccg ccacatgtgg accgtggcga
     3241 ccgcgcgcgc cggatacaat ccccccggca aaggaaagac ccaaccccct gcggattgta
     3301 acggcgaata tgaggagacc tgggaagagc gcgtcgaccg cgatgacctt tggaacctta
     3361 agaccagcga ctgggacgcc acgctgctgc cgctctaccg cgcttggacg caaggggaga
     3421 accggaactg ggatggcact tccggcggta agatcctcgc ccaaatcaaa gagggttcct
     3481 ggcaaccact ttacggtgag ggcggctcgc ccgaaaacga gcgcgccccc aagttgatgg
     3541 gcggcggaaa tgtttcgtac agcgagaccg acgcctgggt ggttcactga accggacacc
     3601 gcccaataaa gaaaggacag aacaatgcaa aggccaaccc gaccttcatt cgagtggaga
     3661 ctccgtatga cgatcttaac cgcatggacc gccttcgccg gactcggcat ggttttcatg
     3721 ccttccgcca gcggagctga caacaccgga ggcaaaacct ccgaaagcga gccaaccgcc
     3781 gaacaagtcg gtgccggtat cctgcatggc ctcacggtca acctgttccg gctgcatgcg
     3841 gcgtcggatg tggacggcgc cgtgaaaaaa tggggcaccg gcgcctttca ggccaagtgg
     3901 acccgcgatg tgcgcgaggt ctttaacgaa catgaa
//
LOCUS       DAOSRB010000246         3899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_20976_length_3899_cov_10.283819, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000246 DAOSRB010000000
VERSION     DAOSRB010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20976_length_3899_cov_10.283819"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PLZ74_10270"
     CDS             complement(<1..1144)
                     /locus_tag="PLZ74_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOR98743.1"
                     /translation="MLLALMLVPGLAVRAQTRVWLDELDLAYMSCGWEHPLAHQAVGG
                     NPLKIARKKFERGVGTHAPSWFAIETGGEALRFEAQVGMDDEELVRGKGSAIFRVYAD
                     GRLAADSGVRHARQPAQHLTAELAGAKVVVLEVSDAGDGNTHDHADWCDAFFTVRGGA
                     VLKPLPRPPTEQMGILTPKPPMAPRINGARVFGVRPGRPVLFTIPVSGEPPFTLTVDG
                     LPEGLALDSARGLITGSVAEAGSYRMTFRAANAHGTAEREWRLEVGERIALTPPMGWN
                     SWNCFAHTVSDANIHTAAQAMVESGLVRHGWSYINIDDYWQTSSSERTDTSLMGPARD
                     SAGRILPNARFPDMAALCRDVHRLGLKIGIYSSPGSTTCGGCVGSWG"
     gene            complement(1182..1469)
                     /locus_tag="PLZ74_10275"
     CDS             complement(1182..1469)
                     /locus_tag="PLZ74_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98744.1"
                     /translation="MRHTASRFICSLRSLRIRILAFAHLALLLPFVLPADTFGPYTYT
                     VSDGQATITDFDSTCSGNLSITNELGGCPVISIAPDPTPRPSRFYRIVFPQ"
     gene            1841..2896
                     /locus_tag="PLZ74_10280"
     CDS             1841..2896
                     /locus_tag="PLZ74_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR98745.1"
                     /translation="MARQLRHHYAGGWYHITSRGMGRKAIFLDDRDREHFVELLSAMV
                     GRYGVVLHAYVLMDNHYHLLIETPAANASRALQWLNLSHSMWFKTRHGHAGPVFQARF
                     KSIPVEGEGSWALTCSVYIHLNPVRVKALGLGKEDRAREKAAFQPEGPPKKQVRARLA
                     KLRGYRWSSYRAYAGYAPSPEWLTCATLWERCCPAAKDPQTEYRRYLEDCLRQGVEED
                     VFVRINAAVAIGGAAFVENLRKRVPRRARGGTNARAWRGMLPFEEVVRAVESVKGMEW
                     ADFAGRRGDWGRDLALYVGRMRCGLTLKGLGEQAGGLGVPAVAKAVERMAARLKTDKA
                     LHRSAARILKLLEKRNV"
     gene            2981..>3899
                     /locus_tag="PLZ74_10285"
     CDS             2981..>3899
                     /locus_tag="PLZ74_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HOR98746.1"
                     /translation="MKKILSCAFLIPLVFTVAAQNVPSLLLNETYRIVTAAQPIPAEE
                     FAATELKAYLDKVTGKTFVIVSEASHDGAPAIHVGETALAAANRNGDCGKEEYHLKAV
                     GKNIIITGGRPRGVLFGTYEFLERFVGVRFLSVNFEHIPNRETIAVPVNLDVRHTPAF
                     IYRDIYPGHAEISAGYRRKVRQNSGGGQARFGFSESYGSPRGVHSMHVYSADFPREIS
                     WMNKNGTRQIVGEPFGGSICFSQPEVLDRFAAKVKDYVRQDRARCKETGAPWPVFYVV
                     DQNDCKVECFCPECQEVVKQHDVSGLVINF"
BASE COUNT          784 a         1108 c         1273 g          734 t
ORIGIN      
        1 gtccccagct tccgacacat ccgccgcatg tggttgagcc cggcgacgag tagattccga
       61 ttttgagacc gagccgatgg acatcgcggc agagcgccgc catatcggga aaacgcgcat
      121 tgggcaggat gcgtccggcg ctgtcgcgcg ccggtcccat gagcgaggtg tcggtgcgtt
      181 cgctagacga ggtctgccaa tagtcgtcga tgttgatgta gctccagccg tgccggacca
      241 agccgctctc gaccatggcc tgcgcggcgg tgtggatgtt ggcatctgaa accgtgtgcg
      301 cgaaacagtt ccacgagttc cagcccatcg gcggcgtgag tgcgatgcgt tcaccgactt
      361 ccaatcgcca ttcgcgctcg gcggtgccgt gcgcattggc ggcgcggaag gtcatgcggt
      421 agctgcccgc ctccgcgacc gatcctgtga tcaggccgcg cgcactatcc agcgcaaggc
      481 cttccggtag gccgtccacc gtcagggtga agggcggctc gccgctgacc gggatggtga
      541 aaagcacggg acggccggga cgtacgccga agacgcgcgc gccgttgatg cgcggtgcca
      601 tcggcggttt cggcgtcagg atgcccattt gctccgtcgg cggacgcggc aacggcttca
      661 gaaccgcgcc gccgcgtacc gtgaaaaagg cgtcgcacca gtcggcgtga tcgtgcgtat
      721 tgccgtcgcc ggcgtccgag acctccagta cgaccacctt ggcaccggcc agttccgccg
      781 tcaagtgttg cgcgggctgg cgggcatgtc gcacgccgga atcggccgcg agccggccgt
      841 cggcgtacac gcggaagatg gcggagcctt tgccgcggac cagctcctcg tcatccatgc
      901 cgacctgcgc ttcgaagcgc aacgcctcgc cgccggtctc gatcgcgaac caactcggcg
      961 catgcgtgcc gacaccgcgc tcgaattttt tccgcgcgat tttcagggga ttgccgccga
     1021 ccgcctggtg cgcaagggga tgttcccagc cgcagctcat ataggcaaga tccagctcgt
     1081 caagccagac gcgcgtctgc gccctgacag ccaagcccgg cacgagcatg agagcgagaa
     1141 gcatgaaata cgaaaaatga ctctttaaca tagcaattaa gttattgggg gaagacaatg
     1201 cggtagaagc gcgaagggcg aggggtgggg tcaggggcga tggaaatgac gggacaaccg
     1261 cccagttcgt tggtgataga taaattgccc gagcaggtgc tgtcgaagtc agtgatggtg
     1321 gcttggccgt cgctgacggt gtaggtgtag gggccgaagg tgtcggcggg taagacaaag
     1381 gggaggagca acgcgagatg cgcaaacgcc aagatgcgaa tgcgcagtga acggagcgag
     1441 catatgaagc gggatgctgt gtgtctcatg gccgtccttt cggttagtgg atgtatggta
     1501 tggtaagacg ggaggcggga agatacaagg ggaaaagagc gaaagaacgc cgaacgccga
     1561 acgctgaact cagaacgttg caactcccaa cgttcaagtg gtgcggcgtg tccgccgccg
     1621 gaccaccgaa agcgagccgg acacaagaca tgtgcggggc ggttcaagac gttccgccct
     1681 actttttgtc gtttcgcggg gaaaaagtag agcaacacac tccgtttgaa actcctaaat
     1741 cccaactcct aactcctaaa tccgtgagaa gaggggtcgt attttgacat ttgacaatca
     1801 ggggttgcgg agcggcttcg cggtgtggta atgtgtcgtc atggcaaggc aactgaggca
     1861 tcactatgct gggggatggt atcacatcac tagccgaggc atggggcgga aggcgatctt
     1921 cctcgatgac cgcgaccgcg agcacttcgt ggagctactg tccgcgatgg tcgggcgtta
     1981 cggggtcgtg ctgcacgcct acgtgctgat ggataatcat taccatctac tgatcgagac
     2041 gcccgccgcg aacgcgagcc gggcactgca atggctgaac ctgagccaca gcatgtggtt
     2101 caaaactcgt catggccacg cgggtcctgt tttccaggcg cgtttcaaga gcatccccgt
     2161 cgagggcgag ggctcgtggg cgctgacgtg cagcgtgtac atccacctga accccgtgcg
     2221 ggtcaaagcg ctggggctcg gcaaagagga tcgtgcgagg gagaaggcag cattccagcc
     2281 cgaagggccg cccaaaaaac aggtgcgcgc gcggctggcc aagctgcggg gctaccggtg
     2341 gagttcttac cgcgcgtacg cgggttatgc gccgtccccg gaatggttga cctgcgcgac
     2401 gctctgggag cgttgttgtc cggcggccaa agacccccaa acggagtacc gtcggtatct
     2461 ggaggactgt ctgagacagg gtgtcgaaga agacgtgttc gtcaggatca acgctgcggt
     2521 cgccatcggc ggggcggctt tcgtcgaaaa cctgcgcaag agagttccga ggcgggcgcg
     2581 cggcgggacg aacgcgcgcg catggcgcgg gatgttgccg ttcgaggagg tcgtgcgcgc
     2641 ggtcgagtcg gtcaagggca tggaatgggc tgactttgct ggccgcaggg gcgactgggg
     2701 ccgcgacctg gctttgtacg tcgggaggat gcggtgcggg ctgacgttga aaggtctcgg
     2761 ggaacaggcc ggcgggttgg gtgtgcctgc agttgccaag gcggtcgaaa ggatggcggc
     2821 gcggttgaaa accgacaaag ccttgcatcg gtcggcggcg cgcatactaa agttgctgga
     2881 aaaacgaaat gtctaatgtc aaactacgac cccaataacc ctgatgtgcc gtggtgatac
     2941 acgggtgccg ttctccgttt ttcaaccaaa aggagcagtt atgaaaaaaa tcctgtcgtg
     3001 tgcgtttttg attccgctgg tgttcacagt tgccgctcaa aatgtcccca gtctgctgct
     3061 caacgagacc tatcgcattg tgaccgcagc ccagccgatt cccgccgagg agttcgccgc
     3121 taccgaactg aaagcctatc tggataaagt caccggcaag acgttcgtta tcgtctccga
     3181 ggctagccat gatggtgccc cggctatcca tgtgggagag accgccttgg ccgcagcgaa
     3241 ccgcaacggc gattgcggca aggaggagta tcatctcaag gctgtcggca agaacatcat
     3301 catcaccggc ggccgtccgc gcggtgttct tttcgggacg tatgaatttc tggagcgctt
     3361 cgtcggcgtg cgttttctga gcgtcaactt cgagcacatt cctaaccggg aaactatcgc
     3421 cgtccccgtc aatctcgacg tccgccacac accggcattc atctatcgcg acatctatcc
     3481 cggacatgcc gagatctcgg ccgggtaccg ccgcaaagtc cgccagaaca gtggcggcgg
     3541 gcaggcccgc ttcgggttca gcgaaagcta cggttccccg cgtggcgtcc actccatgca
     3601 cgtctactcc gccgactttc ccagggaaat ctcttggatg aataaaaacg ggacgcggca
     3661 gatcgtcggt gagcccttcg gcggttctat ctgtttctcc cagcccgaag tcctggaccg
     3721 tttcgccgcc aaagtgaagg actacgtcag gcaagaccgc gctcgctgta aggaaaccgg
     3781 tgccccttgg ccggtcttct acgtcgttga tcagaatgac tgtaaagtcg agtgcttctg
     3841 tccagaatgt caggaggtgg tcaagcagca cgacgtttcc gggctggtca tcaacttca
//
LOCUS       DAOSRB010000247         3890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_21032_length_3890_cov_6.593742, whole genome shotgun sequence.
ACCESSION   DAOSRB010000247 DAOSRB010000000
VERSION     DAOSRB010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21032_length_3890_cov_6.593742"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1227
                     /locus_tag="PLZ74_10290"
     CDS             139..1227
                     /locus_tag="PLZ74_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HOR98747.1"
                     /translation="MTVTLPEQCLHDPVSGIWRTCLPTVARLSSSDTLMLDADSVIRC
                     DGAGEALILELHRRAASQGFTLQCRDARGVVARTLERFRVERFTSSLATPHPVRHTRL
                     CEQLGRTCVHLATGFRDQTAFLGEATVSALSLPRQAGRFRLRDALLHFERAGIDALPI
                     VLLIGFLLGLILAFMSATSFRQFGVEVYVADLIAIGLFRELGPLITAILLAGRSGSAF
                     AAEIGTMKVNEEIDALVTLGLRPVTFLALPRILAATLVMPILSTFSVLAGLLGGVLVM
                     AALGVPPLVFWQHAIRATSLNNVLFGLVKAAVFGLLVGLVGCAHGLQTRRAADGVGRA
                     ATAAVVGSLVLITVADGLFAVLAYLLGV"
     gene            1229..2014
                     /locus_tag="PLZ74_10295"
     CDS             1229..2014
                     /locus_tag="PLZ74_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR98748.1"
                     /translation="MTGSPVIEVEHVCAGYDTALLDDVSFQVNRGEVFVILGGSGCGK
                     STLLKHLIGLIPPLGGTIRLMGDRLFGSPEAERERAMRRFGVTYQSGALFGSMTLLEN
                     VCLPLESFTALPREARDLLAEMKLDLVGLKAFADFFPADISGGMKKRAAIARAMALDP
                     EILFLDEPSAGLDPVTSAELDRLILQLRDTQGVTVVMVTHELASIHAVADRCVMLDKT
                     ARGIIAEGPPAELRDRPPNATVRAFFQREGNRTGASGSHEVSK"
     gene            2011..3123
                     /locus_tag="PLZ74_10300"
     CDS             2011..3123
                     /locus_tag="PLZ74_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOR98749.1"
                     /translation="MNENKANYAKIGFFVLAGMVLMIIVIAIAGSRGLTKRAILAETF
                     FSESVTGLDIGSPVKYRGVPVGEVKHIGFVYSAYREQAHEELTPARARQILVVMALDP
                     ERFGLLGSERADAVLNQLVAQGLRVKIASSGMTGLAFLEMDYFKGQPSEPAPAELQLE
                     THHPYIPSVASTMTALKKAVDDVFVKLSAVDLQALGDEFLATLYLVQHALAHTDVATL
                     AEETTALMTELRESNRSLKGLLDSPELGRLPSTLAATAESARRTIERIESRIDPMTAS
                     VTNVTGRAVCLMEQANGLLEQHGAQIGVTVEALARTAQTLDRTAATQQGDVAALLEDL
                     RRAADGLERIISNLTSAPSALLFGRPPAPLPETRKP"
     gene            3149..3784
                     /locus_tag="PLZ74_10305"
     CDS             3149..3784
                     /locus_tag="PLZ74_10305"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOR98750.1"
                     /translation="MNRITSLCISLIVLAGSSGCLGGARAHQIADRQKRSFLLEASAA
                     QPLAPAPRFGAALLRSLRVQPPFDARTFLIRRGQNEFAADFYHGWIAPPAVLIRSQTA
                     RYLRETGLFGTVFDQPGGTRVPLGLECIVSDLYLDHSGEQPAAVVAMRVLVLDERTTD
                     FPVLFTCEKQARVPLASSDAGTAAEAFGLALTQTLEAVAQSLATAPLSEIR"
BASE COUNT          665 a         1278 c         1184 g          763 t
ORIGIN      
        1 cgcggtttcc gcttttgctc tatccaaccg tccgcttttg aaattcccac gacacaaaaa
       61 acgattgcgg gctttgcccg catctccgac tttgatacgg tataaggatt caactgtgac
      121 gaagtgaaac gctgacccat gaccgtgact ctcccagaac agtgtctgca cgacccggtg
      181 tccggcatct ggcggacttg tcttccgact gtcgcgcgcc tctcttcgtc ggatacgttg
      241 atgctggatg cggacagcgt gatacggtgt gacggggctg gcgaagccct gattctggaa
      301 ctccaccgcc gcgccgcgag tcaggggttc acgctccaat gccgggatgc gcggggggtt
      361 gtggcgcgca cgctcgaacg gttccgtgtc gagcgtttca cctcctccct tgcgacgccc
      421 catccggtgc gccacacgcg gctgtgcgaa cagctcgggc gcacctgcgt gcacctcgcc
      481 accggattcc gtgaccagac tgcgtttctg ggcgaggcca cagtctcggc cctttccctg
      541 ccgcggcaag ccggacggtt ccggttgcgg gacgccctgc tgcatttcga acgcgcggga
      601 atcgatgcgc tgcccatcgt gttgctgatc ggttttctgt tggggctgat cctggctttc
      661 atgtccgcca cctccttccg gcaattcggg gtcgaggtct atgtcgccga tctgatcgcc
      721 atcggcctgt tccgggagct gggcccgctc atcaccgcaa ttctcctcgc gggccgctcc
      781 ggttccgcgt tcgccgccga aatcggcacc atgaaggtga acgaggagat cgacgcgctg
      841 gtgaccttgg gcctgcggcc ggtcaccttt ctggcgctgc cgcgcatcct tgccgccacg
      901 ctcgtgatgc cgatcctcag caccttttcc gttctcgcgg gacttttggg tggtgttctg
      961 gtgatggccg cgctcggcgt cccgccgctc gttttttggc agcatgcgat ccgggcgacc
     1021 tccctgaaca acgtcctgtt cggtctcgtc aaagcggcgg ttttcgggtt gctggtcggg
     1081 ctcgtgggct gtgcccacgg attgcagacc cgccgcgccg cggacggggt cggccgcgcg
     1141 gccactgcgg cagtggtagg cagcctcgtg ttgatcaccg tggcggacgg cctgttcgcc
     1201 gtgctcgcgt atctgctcgg agtgtaacat gaccggatcc cctgtcatcg aagtcgaaca
     1261 tgtgtgtgcc ggatacgaca ccgccctgct cgatgacgtt tcgtttcagg ttaaccgggg
     1321 tgaggtgttc gtcatcctcg gcggatcggg gtgcggcaaa agcaccctgc tgaagcatct
     1381 catcggcctg atcccgccgc tgggcggcac catccggctg atgggcgacc ggctgttcgg
     1441 cagcccggaa gcggaacgtg aacgggccat gcgccgattc ggcgtgacct atcagagcgg
     1501 ggccctgttc ggatcgatga cgctcttgga aaatgtctgc ctgccgctcg aatccttcac
     1561 cgcgttgccg cgcgaagcgc gggatctcct ggcggaaatg aaactcgatc tggtcggact
     1621 caaggcgttt gcggatttct ttccggcgga tatcagcggc ggcatgaaaa aacgcgccgc
     1681 gatcgcgcgg gccatggcgc tggacccgga aatcctgttt ctggacgagc cgtccgcagg
     1741 cctggacccc gtcacctccg ccgaactcga caggctcatt ctccagttgc gcgacacgca
     1801 gggcgtaacc gtcgtgatgg ttacgcatga actcgccagc atccatgcgg tggcggaccg
     1861 ttgcgtgatg ctggacaaga ccgcacgcgg cataatcgcc gaaggtccgc ccgcagaact
     1921 ccgcgaccgg ccgccgaacg ccacagtgcg cgcgtttttc caacgggaag gaaaccggac
     1981 cggggcatcc ggttcacacg aggtgtccaa atgaacgaaa acaaggccaa ttacgcgaag
     2041 atcgggtttt tcgtcctcgc tggcatggtg ctgatgatca tcgtgattgc gatcgccgga
     2101 tcgcgcggtc tgacgaagcg ggccattctg gcggagactt ttttctccga atccgtgacc
     2161 ggtttggaca tcgggtcccc ggtgaaatac cgcggcgtgc cggtcggcga agtcaagcac
     2221 atcgggttcg tgtacagcgc gtaccgggag caggcgcacg aagaactgac ccccgcccgg
     2281 gccagacaga ttctggtcgt tatggctctg gatccggaac gcttcggcct gctgggctcc
     2341 gagcgggccg acgcggttct gaaccaattg gtcgcgcagg gcctgcgggt caaaatcgcc
     2401 agttccggca tgacgggcct cgcctttctg gagatggatt acttcaaggg acagccgagc
     2461 gagccggcac cggccgaact tcaactggaa acccaccacc cctacatccc ctcggtcgcc
     2521 agcaccatga ccgctctgaa aaaagcggtg gatgacgtgt tcgtcaaatt gagcgctgtt
     2581 gaccttcagg ccttgggcga cgagtttctc gcaacgctct atctggtaca gcatgcgctc
     2641 gcccacacgg atgtggccac gctggcagag gaaaccaccg cactgatgac ggaactgcgg
     2701 gaatccaacc gctcgctgaa aggcctgctg gattcgccgg aacttggccg ccttccgtcc
     2761 acccttgccg caaccgccga aagcgcgcga cgcaccatcg agcggatcga atcgcgaatc
     2821 gaccccatga ccgcttcggt caccaacgtg accggccgcg ccgtctgcct gatggaacag
     2881 gcgaacggcc tcctcgaaca gcacggcgca cagatcggcg tgacagtgga ggccttggcc
     2941 cggaccgccc aaaccttaga ccggaccgcc gcgacacaac aaggggatgt cgccgcgtta
     3001 cttgaagatc ttcggcgcgc ggccgatgga ctggagcgga tcatcagtaa cctgacctcc
     3061 gccccgtcgg ccctgctctt cggtcgcccg cccgcaccgc ttccggaaac ccggaaacct
     3121 tgaaacccgc cacacaggaa aaaaccccat gaaccggata acctcgctct gcatctcgct
     3181 catcgtactg gccggatcat ccggctgcct cggcggcgcg cgcgcgcacc agatcgccga
     3241 ccgccagaaa cgctcgtttc tgctggaggc ctccgccgcc caacctctgg cacccgcccc
     3301 ccgctttggc gcggccttgc tacgctccct gcgggttcag ccgccattcg acgcgcgcac
     3361 cttcctgatc cggcgcggcc aaaacgaatt cgccgccgac ttttatcacg gctggattgc
     3421 cccgcccgca gtcctgatcc gcagccagac cgcgcggtat ctgcgtgaaa ccggtctgtt
     3481 cgggaccgta ttcgaccagc ccggcggcac acgcgttccg ctggggctgg agtgtatcgt
     3541 aagcgatctg tacctcgacc actccgggga gcagcccgcc gcagtcgttg caatgcgggt
     3601 gctggttctt gatgagcgca ccacggattt tcccgtgctg ttcacctgcg agaagcaggc
     3661 gcgcgttccg ctggcctctt ccgatgcggg cacggccgcg gaagcgttcg gcctcgccct
     3721 gacgcaaaca ctggaggccg tggcacaatc attggccacc gcaccactca gcgagatccg
     3781 ctaaaccgaa aaagagcagt tgaaccttca accctctttg gtttcaaggt ttcagggttt
     3841 ctgggtttca aggtttcagg gtttcagggt ttcatcccaa tcggggtcgg
//
LOCUS       DAOSRB010000248         3841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_21361_length_3841_cov_5.981511, whole genome shotgun sequence.
ACCESSION   DAOSRB010000248 DAOSRB010000000
VERSION     DAOSRB010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21361_length_3841_cov_5.981511"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PLZ74_10310"
     CDS             <1..1287
                     /locus_tag="PLZ74_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR98751.1"
                     /translation="RVCRMDGARVLVIFRDMTSESREREIRMQMEKRLTKIRKMESLG
                     NLAAGIAHDFNNILAIIQNTVEMTWETPDPKTREAVTTICQATDKGAALTRELMTYAG
                     QTSISFKRDDPNALILDLEKLMGGVVAPNVSIELKLAPNLPQVDTDPHQFWKALINLL
                     KNASEAMDGARGHIRISTYPFTLTEGNLGAFFSTHALSPGNGVVFQIDDTGSGIPREM
                     IAHLFEPFFSTKSVGRGLGLATVFAIIDAHNGGIAIDSDLGKGTTFRIWLPAADKPAD
                     EDRAEVRNALQTASPSALRKTAEAQSPAAPPSIPRVLLIEDDPAILLSTAIVLRSLNV
                     DPLTAATKREALAVFRKQADSIALVLLDAHIDHLDNVRLLSTLRLHNPKVPVVVISGY
                     NEKRIREMFASETYEGFLAKPYTRDELKSMLERFIL"
     gene            complement(1459..2340)
                     /locus_tag="PLZ74_10315"
     CDS             complement(1459..2340)
                     /locus_tag="PLZ74_10315"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98752.1"
                     /translation="MAEQEQKMSEAIAFFEQMLQTMPDDRTSLEFLSVAYEQTGQTEK
                     RRDCLIRLADTLLREKDYDNAQVIAGYLSAFTDDPGARAAVERVAELVQGQILKSEFQ
                     REVAGLAGAVSGAAAEVPGSGLPSFQETGVEVHALSRSASAAEMDLVWLWKENEFLPK
                     ETCMDVLHVLTERPVTDTPVLISALALLDEQHPELTERVMESMHRLSEVPPLPIELFE
                     PQPEAVALLPQVFIHVKGVLPFALLGGEVLVAVLNPLNRDLQAEVSARIGKPCHFYLV
                     HPKMWQHVFSKIVPAAV"
     gene            2580..>3841
                     /locus_tag="PLZ74_10320"
     CDS             2580..>3841
                     /locus_tag="PLZ74_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOR98753.1"
                     /translation="MPQHIHYPSFQSALLRRGVLSEEQLQKAQEEAGKVPLERFLLQN
                     RLVPPDVFALTAAAYFNISPITLPPGLILPRELLDLASPQLWARLKAAPLLRLGKRLT
                     VAFGDPFDLLAQEKVARVTRLEIVPLVAPEKEVMDVLGRVQSQRDAENPDLAMENIMK
                     VSDADIEFSSEPQHQEENIDKTLETAEEAPVIRMVNMMLVEALRIGASDIHFEAMEKS
                     SRLRYRIDGALIERPSPPKPLHNAIVSRIKIMSDLDIAERRNPQDGRFKVRALKKEVD
                     IRVSILPTIHGEKVVMRTLDKSNLAPSLAALGLDDFAFKAMKFAIDQPHGIILVTGPT
                     GSGKTTTLYSCLQDLNQPDVNIITAEDPVEYQLPGVNQVHVNQQTGLTFASILRSVLR
                     QDPDIVLVGEIRDGETADIAVKAALTGHL"
BASE COUNT          811 a         1262 c         1092 g          676 t
ORIGIN      
        1 cgcgtctgcc ggatggacgg ggcccgcgtg ctggtgattt tccgcgatat gacctccgag
       61 agccgtgaac gcgagatccg gatgcagatg gagaagcgcc tgaccaagat ccggaagatg
      121 gagagtctcg gaaatctggc ggccggcatc gcgcatgact tcaacaatat cctcgcaatc
      181 atccagaaca cggtggagat gacgtgggaa acgcccgacc cgaaaacacg ggaggcggtc
      241 acaaccatct gccaggccac ggacaagggt gccgcgctga cgcgggagtt gatgacctac
      301 gccggccaga cgagcatctc cttcaaacgc gacgatccca acgccctgat tctcgatctg
      361 gagaaactca tgggcggcgt ggtcgccccc aacgtctcca tcgaactgaa gctagccccg
      421 aacctgcccc aggtcgacac ggacccgcat cagttctgga aagccctgat caacctgctc
      481 aagaacgcct ccgaggcgat ggacggtgcc cgcggccaca tccgcatcag cacttacccg
      541 ttcacgctta ccgaaggcaa tctcggcgct tttttcagca cacacgcgct atccccaggc
      601 aacggagtcg tattccagat cgacgacacc ggttccggca tcccgcgcga gatgatcgcc
      661 cacttgttcg aacccttctt ctcgaccaaa tccgtgggcc gcgggctggg gctggcaacg
      721 gttttcgcga tcatcgacgc gcacaacggc ggcatcgcca tcgattccga cctcggcaag
      781 ggcacgacct tccgcatctg gcttccggct gcggacaaac ccgcagacga agaccgcgcg
      841 gaggtccgga acgcccttca gacggcatcc ccctccgcac tccgcaaaac ggccgaggcg
      901 caatccccgg ccgcgccgcc gtcgatcccc cgtgtcctgc tgatcgagga cgatccggcg
      961 atcctgctca gcaccgccat cgtcctgcgc tcgctgaacg tcgacccgct gaccgccgcc
     1021 accaaacgcg aggctctggc ggttttccga aaacaggccg actccatagc cctggtgttg
     1081 ctggacgcgc atatcgacca tctcgacaac gtgcggctgc tctcgaccct gcgcctgcac
     1141 aacccgaaag tgccggtcgt ggtgatctcg gggtacaacg agaaacgtat ccgggagatg
     1201 ttcgcctccg agacgtacga gggcttcctg gccaagcctt acacccgcga cgaactcaag
     1261 agcatgctcg aacgctttat cctataataa ataataaacg gcaggtgagc gtgcgaactc
     1321 gttttcgtgt acgtggactt gcgacccgcg acattcgact tgcggcgttt gggttgccgg
     1381 caagacgacc gatccctccg atccgccttt gcgggcgaag acgcccgttt ttgattggcc
     1441 gtatcgaccg acaaggcgct agacagcagc gggcacgatc ttggaaaaca catgctgcca
     1501 cattttgggg tgcaccagat agaagtggca cggtttgccg atgcgcgcgg acacctcggc
     1561 ctgcagatcc cggttcagcg gattcagcac cgcaaccagc acctcgccgc caaggagcgc
     1621 gaaaggcaga acccccttga catggatgaa cacctggggc agcagcgcga ccgcctccgg
     1681 ttggggctcg aacagttcga tcggcagagg cgggacctcg ctcaaacgat gcatcgactc
     1741 catcacccgt tccgtcagtt ccggatgctg ctcgtccaac agcgccaggg ccgagatcaa
     1801 aaccggcgtg tccgtcaccg gacgctccgt cagcacatgg agcacatcca tgcaggtctc
     1861 cttcggcagg aactcgttct ccttccagag ccaaaccaga tccatttccg ccgccgaggc
     1921 ggagcgcgaa agcgcgtgca cctcgacccc ggtctcctga aaggacggca gaccgcttcc
     1981 cggcacctcc gccgccgcac cggacaccgc gccagccagc ccggcaacct cccgctggaa
     2041 ctcgctcttg agaatctggc cctgcaccag ctccgccacg cgctccaccg cggcgcgtgc
     2101 gccggggtca tccgtaaacg cgctcagata cccggcgatc acttgggcgt tgtcataatc
     2161 cttctcgcgc aacagcgtat ccgccagccg gatcagacaa tcgcgccgtt tttccgtctg
     2221 gccggtctgc tcataagcca cggacaggaa ctcgagactc gtccggtcat ccggcatggt
     2281 ctgaagcatc tgctcgaaaa acgcgatcgc ctcgctcatt ttctgttctt gttccgccat
     2341 gtcagcctaa actccttctg cttttttatt taagcagaaa acatgccgac ccggatttcc
     2401 gggcagaaag agggcgcgcc cctgttgacc ggatcgattc tggcacctta tctgctaata
     2461 gatgttacgc ctctttcggt caggggagta ctcgaacaac cggtgcaaat gtccgggaaa
     2521 gcgctcctgc cgcagatggc atggacaagg gacagaatac cggaaaaccg gaaaaaacga
     2581 tgccgcaaca catccattac cccagtttcc agagtgcgtt gctccgacgc ggggtgctct
     2641 cggaagagca gcttcagaag gcacaggaag aagcgggcaa agtcccgctg gagcggtttc
     2701 tgcttcagaa ccggctggtt ccgccagatg tgttcgcgct gacggccgca gcctatttca
     2761 acatttcgcc catcaccctg ccgcccggcc tgatcctgcc gagggagttg ctcgatttag
     2821 cctcgcccca actctgggcc agactgaaag ccgcgccgct cctgcgtctc gggaaacggc
     2881 tgaccgtggc tttcggagac ccgttcgatc tgctggccca agaaaaggtg gcgcgggtca
     2941 cgcggctgga gatcgtgccg ctcgtggcac cggaaaaaga ggtgatggac gtgctggggc
     3001 gggtccagag ccagcgggac gcggaaaatc ccgatttggc catggaaaac atcatgaagg
     3061 tgtcggacgc cgacatcgag ttctccagcg agccgcagca tcaggaagag aacatcgaca
     3121 agacgctgga gaccgcggag gaggcgccgg tcatccgcat ggtgaacatg atgctggtcg
     3181 aggcccttcg catcggtgcc agtgacatcc atttcgaggc catggaaaaa agctcccgcc
     3241 tacgctatcg gatcgacggg gcgttgatcg agcgccccag cccgccaaaa ccgcttcaca
     3301 acgcgatcgt ctcgcgcatc aaaatcatgt cggaccttga tatcgcggag cggcgcaacc
     3361 cgcaggacgg ccggttcaag gtccgggcac tcaagaaaga ggtcgatatc cgcgtgagca
     3421 tcctgcccac cattcatggc gaaaaggtgg tgatgcgcac actcgacaag agtaacctcg
     3481 cccccagtct ggcggcactc ggcttggacg atttcgcctt caaggccatg aagttcgcga
     3541 tcgatcagcc acacggcatc attttggtga ccggcccgac cggatcgggc aaaaccacaa
     3601 cgctttactc ctgcctgcag gatttgaacc agccggacgt caacatcatc acggccgagg
     3661 atcccgtgga gtatcaactc cccggtgtga accaagtcca tgtcaaccag cagaccggcc
     3721 tgactttcgc ctcgattctg cgctccgtgt tgcggcagga tcccgacatc gtgctcgtcg
     3781 gtgaaatccg cgacggcgag accgcggaca tcgcggtcaa ggccgcgctc acgggccatc
     3841 t
//
LOCUS       DAOSRB010000249         3830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_21442_length_3830_cov_8.210066, whole genome shotgun sequence.
ACCESSION   DAOSRB010000249 DAOSRB010000000
VERSION     DAOSRB010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21442_length_3830_cov_8.210066"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1076
                     /locus_tag="PLZ74_10325"
     CDS             114..1076
                     /locus_tag="PLZ74_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR98754.1"
                     /translation="MTILWLAGNLLHTGWVETRRFFWERRVTRDPEGLLPEAAAFQVG
                     EGPIALLFIHGFADTPRVWRRFADRLAATGDFTCRALRLAGSAEPAAQAKRASLELWR
                     MQLSDELIRLGEHHKRIWVAGHSMGGALALDTALRQPEAVEGVAALAPLIEVSRARAP
                     VLAPEVWFALARVALCLSPTFESPFSTQAVAADDPDFTYTRDRFIPFSVYQSLFRLAR
                     SNRKALGRLTQPVFAVTANRDVVVDTSAALRWLAECRGPKRILDLQDVGHVIPLENGW
                     RAVADDLAAFIRDHTRQEGRRLPEASADSSGELSLPESEETPPA"
     gene            1101..2042
                     /locus_tag="PLZ74_10330"
     CDS             1101..2042
                     /locus_tag="PLZ74_10330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOR98755.1"
                     /translation="MKPPFVSVIIPTYNRAQTACDCVASVLRSDYRAIEVIIVDDASP
                     DNTRELVERQFGNDPRVRYVRNEKNLQAAAVRNRGASLAKGENLLFVDDDNLVEPDMV
                     SKLVHGMSLDSGLGQIAPLAIHKAPNLIWTLGSTFNLWTSQPKNLNEREPADRVALED
                     RLYPSWYSPNVWMVKKEAFDRIGGYDPFYRIMFDESDFGMRLRRAGYRSAIYPMARTY
                     HLGFIEPGKTAALRCLGIESPERAYCFARNRSVFVRRHFPPVNRAVVLTVFVPLFAVY
                     YGAVALRHRRPDIAWAFFKGSFVGWFSFPKEPQGTDA"
     gene            complement(2192..3700)
                     /locus_tag="PLZ74_10335"
     CDS             complement(2192..3700)
                     /locus_tag="PLZ74_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOR98756.1"
                     /translation="MDGKKVFAVDLGASGGKCFVGVFSDKGFRMEEIHRFAHEGVSCF
                     LPDRSGEVTERTFWDDTFIYQNIVKGLQAARRQCGAVLDGIGIDTWGADGNLVTRDGD
                     LLGKVYCYRDHRLDAMCEEVKARLDATKVYEITGNHFQPFNQSNQLLWLATRRPELLK
                     LAEVYLPIPSLFYYYLGGCKVVDSTFASVTQMMDAKRGKWSREMLKALGIPKRLMPEI
                     VKPGTKVGRLQPKLAAACGLNEADLIAVGSHDTASAFAAAPVRNVKTALIISSGTWSL
                     VGKLIRKPITTPEAMAAGLSNEGGIGNTRFLRNCMGTWIVQELLRVWEIADGKRMPWS
                     EVDTLTPAAPAFTAFIDPDDSRFYNPANMEQEIRAYLNETGQPAPVDRGTVLRCVYES
                     LALKYRQVNETINRVTGTDTEAVHIVGGGSNNALLNQFTADSVGVPVFAGPKEATAVG
                     NLMVQAVGAGILPDLKAAKSIIRASFPITVFQPQQTDAWDAAYARFSSFVHY"
BASE COUNT          703 a         1207 c         1144 g          776 t
ORIGIN      
        1 gaaaccaaag agagttgaag gttcaactgc tcttttaggc taatttgttc agccgccttg
       61 tgcggagaac gcgggtatag tattgtttgg atatgttata cgggttggct tgtttgacaa
      121 ttctctggct ggcgggaaat ctgctccaca ccggatgggt ggaaacacgg cgcttctttt
      181 gggaacgccg cgtcacgcgt gatccggaag ggctcctgcc cgaggcggcg gcgtttcagg
      241 tcggggaggg tccgatcgcc ctgcttttca tccacgggtt cgcagatacg ccccgtgtct
      301 ggcggcggtt cgcggatcgg ctcgccgcaa cgggtgactt cacctgccgc gcgttgcgcc
      361 tggcggggag cgcggagccc gccgcacagg cgaagcgcgc gtcacttgaa ctctggcgga
      421 tgcaactttc cgatgagctg atccggctgg gcgaacatca taagcgtatt tgggtggcgg
      481 ggcattcgat gggcggcgcg ctcgcgctgg acaccgccct gcgccagccg gaagcggtgg
      541 aaggcgtggc ggcgctcgcc ccgctcatcg aggtttcacg tgcccgcgca cccgtgttgg
      601 cacccgaggt gtggttcgcc ctcgcccgtg tggcactttg cctctcgccc acgtttgaat
      661 cgcccttctc cacgcaggcc gtggcggcgg acgatcccga ttttacctac acgcgtgacc
      721 gcttcatccc gttttccgtg taccaaagcc tgttccggct ggcgcgctcc aaccgcaagg
      781 ccttgggccg cttgacgcaa cccgtgttcg ccgtaaccgc caatcgggat gttgtggtgg
      841 acacatcggc tgcgctgcgg tggctggccg agtgccgcgg cccgaaacgg attctcgacc
      901 tgcaggatgt cggccacgtg atcccgctgg agaacggctg gcgtgctgtc gcggacgatc
      961 tcgccgcctt catccgggat cacacgcggc aggaggggag acgcctcccc gaggcgtccg
     1021 cggacagttc gggggagctg tccctaccag aatcggagga gacgcccccc gcttaatcgg
     1081 cgacaggaga accttttcgt atgaaaccac cctttgtatc cgtcatcatc ccaacataca
     1141 atcgcgcgca gaccgcgtgc gattgcgtgg cctctgtttt gaggagcgat taccgcgcga
     1201 tcgaagtcat catcgtggat gacgcctcac cggataacac ccgcgaactc gttgaacgtc
     1261 aattcggcaa cgatccgcgc gtccggtacg tccgcaacga gaaaaacctt caggccgccg
     1321 cagtccgaaa ccggggcgcg tctctggcaa agggagagaa ccttcttttc gttgacgacg
     1381 acaacctcgt ggaacccgac atggtttcca aactggttca cggcatgtca ttggacagcg
     1441 ggttggggca gattgcgccg ctcgcgatcc acaaggcgcc gaacctgatt tggacgctgg
     1501 gctcgacctt caacctgtgg acctcccagc cgaagaacct caacgagcga gagcctgcgg
     1561 accgcgtcgc gctggaagac cggctctatc cctcctggta cagcccgaat gtgtggatgg
     1621 tcaaaaagga ggccttcgac cggatcgggg gatatgatcc cttttaccgg atcatgttcg
     1681 acgaaagcga cttcggcatg cggctccggc gtgccgggta ccggtctgcc atttacccca
     1741 tggcccgcac ctaccacctc ggattcatcg agcccggcaa aaccgcggcc ctgcggtgcc
     1801 tcggcatcga gagccctgag cgcgcttact gtttcgcgcg caaccgctcc gtttttgtcc
     1861 gtcggcattt cccgcccgtg aaccgcgctg ttgtcctcac ggtttttgtt ccgttgttcg
     1921 ccgtctatta cggggccgtg gccttgcggc accgccgccc ggacattgcc tgggcgtttt
     1981 tcaaagggag cttcgtcggc tggttctcct tccccaagga accgcagggt acggacgcct
     2041 gatgatcccc aatgcggaca gagtccccga cccagagagg gggacggggg gattgccggt
     2101 tttatcgcta tcagaccaaa aggctgcaag gcggtgaggc ggttgtgttc tgtggtgaaa
     2161 caatcgatcc cgatagcgat cccgatcccg actaataatg cacgaatgaa ctaaagcgcg
     2221 cataggccgc atcccacgca tcggtctgct ggggttggaa aaccgtgatg ggaaaagaag
     2281 cccggatgat cgacttcgcg gctttgaggt cgggcaggat gcccgcgcca accgcctgca
     2341 ccatcaggtt gccgaccgcc gtggcctctt tcggtccggc gaaaaccggc accccgacgg
     2401 aatcagcggt gaattggttg agtagcgcat tgttgctgcc gccgcccacg atatgcaccg
     2461 cctcggtatc cgtgccggtg acacggttga tcgtctcgtt gacctgacgg tatttgagcg
     2521 ccagactttc gtacacgcaa cgcagcaccg tgccgcggtc aaccggcgcg ggctgtcccg
     2581 tctcgttcag gtaggcgcgg atctcttgct ccatgttggc cggattgtag aaacgcgaat
     2641 catcgggatc gatgaacgcc gtgaaggccg gcgcggccgg cgtgagcgtg tccacctcgc
     2701 tccacggcat gcgcttgccg tcggcgatct cccacacgcg cagcagttcc tgcacaatcc
     2761 aggtgcccat gcagttacgc agaaaacgcg tgttgccgat gccgccctcg ttgctcagac
     2821 ctgcggccat ggcctccggc gtggtgatgg gcttccggat gagtttcccg accagagacc
     2881 aggtgccgga actgatgatc aacgccgtct tgacgttgcg caccggcgcg gccgcaaagg
     2941 cactggcggt atcgtgcgaa ccgaccgcga tcagatccgc ctcgttcaga ccgcaggctg
     3001 cggccagctt gggttggagc cgaccgacct tcgtgccggg cttgacgatc tcgggcatca
     3061 gccgtttagg gatacccaac gccttgagca tctcgcggct ccatttgccc cgtttggcgt
     3121 ccatcatctg cgtgacactg gcaaaggtgg agtccaccac cttgcatccg cccagataat
     3181 aatagaagag cgacgggatg ggcagataaa cctcggccag cttgagcagt tcaggccgac
     3241 gcgtggcaag ccagagcaac tggttcgact ggttgaacgg ctggaaatgg ttgcctgtga
     3301 tttcataaac ctttgtggca tccaaacggg ctttgacctc ctcacacatg gcatcgagcc
     3361 ggtggtcacg gtaacaatag accttgccga gcagatcgcc gtcccgcgtc accagattgc
     3421 catccgcgcc ccaagtgtcg attccgatgc cgtccagcac ggcaccgcac tggcgccgcg
     3481 cagcctgaag acctttaacg atattctggt agataaaggt gtcgtcccaa aatgtacgct
     3541 ctgtgacctc gccagaacgg tccggcagaa aacaggacac gccctcatgc gcgaaccggt
     3601 gtatctcttc catccggaaa cccttgtccg aaaacacgcc gacaaagcat tttccgcctg
     3661 acgcacccaa atccaccgca aacacttttt tgccgtccat gctgacctcc tcaacaccct
     3721 ctttgtttcc gcgtcttcac ctgaaaacgc gcgggacctc ctgcccgtga acttcccgtc
     3781 gcttcagccc gcttcccgcg tgtggctgac ctttgtgtgt gagtataccg
//
LOCUS       DAOSRB010000250         3825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_21471_length_3825_cov_8.299735, whole genome shotgun sequence.
ACCESSION   DAOSRB010000250 DAOSRB010000000
VERSION     DAOSRB010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21471_length_3825_cov_8.299735"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLZ74_10340"
     CDS             complement(<1..367)
                     /locus_tag="PLZ74_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98757.1"
                     /translation="MDIHADSYRDGDNSNSGLRMKLSGTREVWVRLAGLAVVLGWGAS
                     LRAATQDGALRVEVNSGYNLIVASNAGSSSSYVPGAAYIGVTLHNDGTTDLTDIVVCA
                     GNYNGGTGSTPGIYPSRTHD"
     gene            806..1384
                     /locus_tag="PLZ74_10345"
     CDS             806..1384
                     /locus_tag="PLZ74_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOR98758.1"
                     /translation="MASIKGTQTEKLVLTAFTGESAARNRYTFWAGQAKKEGFVQIEA
                     IFEETANQEKEHAKRLFKFLEGGDVPIEITLPAGVIGKTEENLIHAAEGEEYEWQHMY
                     PDFAAVARKEGFEEIAKTMEAIAIAEKFHGERYRQLAANIKKGIVFKREDPNTIWRCR
                     NCGYLHVGAEALDLCPACAHPKAHFELRGINW"
     gene            1432..1797
                     /locus_tag="PLZ74_10350"
     CDS             1432..1797
                     /locus_tag="PLZ74_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOR98759.1"
                     /translation="MALASVFEEDLEESFILGSGPGGQKINKTASTVRLLHRPSRLTV
                     KCGVSRSREQNRWLARRELAERILERERSELSIRRQAAEKIRRQKRRRSRRQQARMLD
                     DKRKHSEKKALRGAVRFDA"
     gene            1836..3647
                     /gene="recJ"
                     /locus_tag="PLZ74_10355"
     CDS             1836..3647
                     /gene="recJ"
                     /locus_tag="PLZ74_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOR98760.1"
                     /translation="MFPKYRWRDSPVEDGSARELADALELPLPLARVLAARGHTSPDQ
                     AAAFLAPNLQAHLGHPDGFPGAAEAAERLWAAVRAGRRIVVYGDFDADGVAAAAVMVS
                     SLKRFGGRVSVFLPHRDPEGYGLTLAALERCRREQGGWPDLLVTVDCGITSINEVEHL
                     NAAGVEVILTDHHEPGTVLPHAAVIVNPRVGASPGAEHLCGAGVAFKVIHAMAELGKR
                     RNWYPGEPFCGELLAPVGVATVADVVPLTGENRLLVSAALRSWERYAGAGLRALLARA
                     SAQTVRDMDAALFGFVLGPRLNAAGRMGSAMDAYELLATCDRQRATELAARLEGFNGE
                     RRGVEARMLAAARHQCGLDSPQGLGDLAAVVVGGDGPHAGATGWHPGVLGIVAARLSE
                     AAARPAAVVAFDETGAGRGSVRAGEGYHALKALAAAGDALTGFGGHERAAGFQVRAGG
                     FAQFRQRFCEACADQARTADGGPCLMVDGWLTPESLSVAFLAAQQRLAPFGYGNPIPR
                     WGLRGVSLERAQPVGNSGEHLQLAFDVGAGRSVRGVWFRHGAMSEAVRAAGGRMDVVF
                     EMRRNDFGGEASVEMRICDLAPTVAEKEVRGRLKKSS"
BASE COUNT          688 a         1079 c         1336 g          722 t
ORIGIN      
        1 catcatgcgt ccgcgaggga tagatacccg gcgtggagcc ggtcccgcca ttgtagttgc
       61 cggcgcacac cacgatgtcg gtcaggtcgg ttgtgccgtc gttgtgcagc gtcacgccga
      121 tgtaggccgc gcccggcaca taggacgagg acgagcccgc attcgaggca acgatcagat
      181 tgtagccgct gttgacctcg acccgcagcg caccatcctg tgtggccgcg cggagggatg
      241 cgccccaccc cagcaccacg gcaagccccg ccagccgcac ccagacttcc ctcgttccgc
      301 tgagcttcat tcttaaaccg ctgttggaat tgtcgccgtc ccgataagag tctgcgtgaa
      361 tatccacgtt tcaccccttc gctgataggt ctgacaggtc aagctcggtt tgacaaaatt
      421 gcgctgaaca tactccttta atcgggtgtt tgcagcgaca ccccctgcca tttacttttt
      481 tatgatacaa aaacaggtgt gtaaggtcta taaaaaagtg tgaggatttt gaatttttgt
      541 gtgaaatccc gtcagcctct ccgcgctatg ctcggagcca aaattcgcgt cttggccgat
      601 aaggccaata aaaacaagtt gtttatcgcg gcaggggcgc ccgataacgg acgcccctgc
      661 cgcgataata ggcagatgaa tcgggaatgg actcttgcgg agaccgttta cgtgattgcc
      721 cggaattacg gttttctgat aagatcatcc cgttttcaaa gaacacgatt caagtccagc
      781 catgagtcaa aaggaggaat gcaagatggc atcgatcaaa ggcacgcaga cggagaaact
      841 ggttctgacc gctttcacgg gggaatcagc agcgcgaaac cgttacacgt tttgggcggg
      901 gcaggccaag aaggaagggt tcgtccaaat tgaggcgatt tttgaggaga ccgcgaatca
      961 ggaaaaagag catgccaaac ggctcttcaa atttttggag ggcggcgacg tgcccatcga
     1021 gatcacactg cccgcaggcg tgatcggcaa gaccgaggag aacctgatcc atgcggccga
     1081 gggcgaggag tacgagtggc agcacatgta tcccgatttc gccgccgtgg cgcgcaaaga
     1141 ggggttcgag gaaatcgcca agaccatgga ggcgatcgcg attgcggaga agttccacgg
     1201 cgagcgttac cgccaactgg ccgccaacat caagaagggt atcgtcttca aacgcgaaga
     1261 ccccaacacg atctggcgtt gccgcaactg cgggtatctg catgtcggtg cggaagccct
     1321 ggacctctgc cccgcctgcg cccatcccaa ggcgcatttc gagttgcgcg gcatcaactg
     1381 gtaagggatg ccctgatcac ccccggaacc atcgagaaga tccgtgcgcg catggctctg
     1441 gccagtgtgt tcgaggagga tttagaggaa tccttcattc tcggatcggg ccccgggggc
     1501 cagaagatca acaagaccgc ctccacggtg cggcttctgc accgccccag ccgactcacg
     1561 gtcaaatgcg gcgtcagccg gtcgcgtgag cagaaccgct ggctggcgcg gcgtgaactg
     1621 gctgaacgta ttttggagcg cgagcgttcg gaactctcga ttcgacggca ggcggcagag
     1681 aagatccgac gccagaaacg ccgacgctcg cggcgtcagc aggcccgcat gctggacgac
     1741 aagcgtaaac acagtgaaaa gaaggcgttg cggggcgccg tgcggttcga cgcctgaacg
     1801 ccgcaggggc cgtttcgggc gaggcggggc gcggcatgtt tccgaaatac agatggcggg
     1861 attcgcccgt agaggacggg tccgcccgcg aattggctga cgcgttggag ttgccgttgc
     1921 cgctggcacg ggttttggcg gcgcgcggcc acacctcgcc ggatcaggcc gccgcgtttc
     1981 tggcgccaaa cttgcaggcg catctggggc atccggacgg gtttccgggt gctgcggaag
     2041 ctgcggaacg gctctgggcc gcagtccggg ctggccgcag gattgtggtc tacggggatt
     2101 tcgatgcgga cggcgtggcg gccgcggcgg tgatggtttc ctcgctcaag cggttcggag
     2161 gtcgggtttc ggtctttctg cctcaccgcg atccggaggg ctacggcctg accctcgccg
     2221 ccttggagcg ctgccgccgc gaacaggggg gatggccgga cctgctggtc accgtggatt
     2281 gcggaatcac ctcgatcaac gaggtggagc acctgaacgc cgcaggggtt gaggtgattc
     2341 tgaccgacca ccacgaaccc ggcacggtat tgccccatgc cgcagtgatc gtgaatccgc
     2401 gtgtcggggc ttcgccgggt gcggagcacc tgtgcggcgc aggcgtggct ttcaaggtga
     2461 ttcacgcgat ggccgaactg ggtaaacgcc gcaactggta tccgggcgaa cccttctgcg
     2521 gcgaactgct ggcaccggtc ggggtggcga cggtcgcgga cgtggtgcca ttgaccgggg
     2581 agaaccggct gttggtttcg gccgcgctcc gttcgtggga gcggtacgcg ggtgcgggat
     2641 tgcgcgcgtt gctggcgcgc gcgtcggcgc agaccgtccg cgacatggat gcggccctgt
     2701 tcggatttgt gctggggccg cggctcaatg cggccggccg tatggggtcg gctatggatg
     2761 cgtacgagtt gttggccacg tgtgaccgtc agcgcgcgac ggagttggcg gcgcgtctcg
     2821 aagggttcaa cggcgagcgg cgcggcgtgg aggcgcgtat gctggccgcc gcgcggcacc
     2881 aatgcggact tgattcgccg cagggactgg gcgacctcgc ggcggtggtg gtcggcggcg
     2941 acggtccgca tgccggggca accggctggc accccggagt actcgggatc gtggcggcgc
     3001 ggctgagcga agcggcggcg cggcccgcag cggtggtggc gttcgacgag acgggcgcgg
     3061 gtcggggttc ggtgcgcgcg ggcgaggggt atcatgcgct caaggctctg gccgcggccg
     3121 gggatgcgct gaccgggttc ggcggacacg agcgggccgc agggtttcag gtacgggccg
     3181 gcgggttcgc gcagttccgg cagcggtttt gtgaggcgtg cgcggatcag gcgagaacag
     3241 ccgacggcgg gccatgcctg atggtggacg gatggctcac gcccgagtcg ctctccgtcg
     3301 cgttccttgc cgcgcaacag cgtctggcac cgttcggcta cggcaacccg ataccgcgct
     3361 ggggtctgcg cggggtgagc ttggaacgcg cgcaaccggt cggcaactcc ggcgagcatt
     3421 tgcaactcgc gtttgatgtg ggcgcgggcc gctccgtgcg gggcgtatgg ttccgccacg
     3481 gcgcgatgtc cgaggccgtg cgcgccgcag gcgggcggat ggatgtggtg tttgagatgc
     3541 gccgcaacga ttttggcggg gaagcgtcgg tggaaatgcg aatctgtgac ctcgcgccga
     3601 cagtcgcgga aaaggaggtc cgcggacgcc ttaaaaagag cagttgaacc ttcaactctc
     3661 tttggtttca gggtttcagg gtttcagggt ttcaaggttt catcggggtc ggggtcggaa
     3721 tcgggatcgg gatcgattgt ttaaccacag gacactttct tgggtttcag ggtttcaggg
     3781 tttctggttt catggtttca gggtttcatc caaatcgggg tcggg
//
LOCUS       DAOSRB010000251         3815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_21563_length_3815_cov_8.822074, whole genome shotgun sequence.
ACCESSION   DAOSRB010000251 DAOSRB010000000
VERSION     DAOSRB010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21563_length_3815_cov_8.822074"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1350)
                     /locus_tag="PLZ74_10360"
     CDS             complement(316..1350)
                     /locus_tag="PLZ74_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98761.1"
                     /translation="MKYPFEADYQEILADSDRFVTAIVTSLASEFLTLPKGKGFIEYP
                     QFAAGYEDLKKVTTGFSKLPRREIVDLVASTPVCFIVIRSILGFTPSEWAYVASQRRG
                     AAVTQGFARTLDRNIRMQPLKPLKCRGLTRERLTALLDAACDLLQEGCPKPNDITIHR
                     LQKADTKEGLQSIKSLARMGAPYAMLLYERFLGRPFAGHRDSVSELVGDSLESAIEDV
                     LSKAGVSYRKTKRAERVPGFDQAPDFIVPDEFTPRVVIEAKITEDDGTARDKVTRIQH
                     LGELSTAGAPVGQPKYQVIACIAGRGFGVRREDMRKMLFATRGKVFTLKTLNRLVECS
                     DLAKFATKPY"
     gene            complement(1334..3538)
                     /locus_tag="PLZ74_10365"
     CDS             complement(1334..3538)
                     /locus_tag="PLZ74_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOR98762.1"
                     /translation="MIERDFDVPFVADLALREKQIQQNYRPVVAVHKWFARRPGTLFR
                     ALLLSEFLEGPLREQFYRGQSLKHLRVLDPFMGGGTTLLEANRVGCSVTGFDINPMAW
                     WIVRQEIGELNPRAYRVAADTLRDTLESEIGHLYRTQCRECGARDAHVKYFLWVKSPP
                     CAKCRQPVDLFPNYLVAADVRHPTNLFVCRDCGELYDSPARKNPPGCPHCKTRFSVEF
                     TARRNRCKCKHCGHVNAYPTPEGGPPPHRMFAIEYHCERCRPAHSGRFFKKPAPADLR
                     RYDEAAARLAAGEARFIPQEEIPPGDETDRLHRWGYRFYREMFNARQLLGLELLCHAI
                     KRQKDSRIADALATNLSDLVRYQNLLCRYDTKALKSLDVFSVHGFPVGLIQCESNVLG
                     ISGKAMAVGSGGWLNIVDKFAKAKAYCRHPFEIRHEGRRKVRVPILTEWIGDQRNDLH
                     PPETKEVALACADASEADFTGRKFDAILTDPPYFQNVQYAELMDFCYVWLRKLVKAAH
                     PEFDGLTTRHTNELTGNVSMGRTLEHFTEGISRVFRRMAKALNPGAPLAFTYHHNSLD
                     AYLPVAVAMLDSGLACSASLPCPAEMGASIHINGTVSSIVDTVFVCRSTGKFPRRWLA
                     DTPAALARVAAGDLRLLEQGQMKPTQGDMRCVVFGHLIRLAVWRLRPTWVATPKAADR
                     MAAVRRWIIKFGGADAVLQELGDAYARARAEQQWELNEEDKAFRTSTDEISF"
BASE COUNT          793 a         1127 c         1175 g          720 t
ORIGIN      
        1 ctgcaattcc aagcctcata accctcgccc caaaaccgcc tttgccgcgc ccgctgataa
       61 ccggacgctg cacaggtaat ttcttacgca ctccgctcgc gaatgttccg aatcccgggg
      121 aaaggaggcg catcttgccg cccctccacc agccaacatc gcggccggag cggccctccc
      181 aagcggaggc cgcctgggcg cggaagccag caacgctatc acgcgcaccg cggttcccgc
      241 gggagcactt ggaatgcagg tatttgcaaa gatcgaggcg ggcaaaggtc ataacggatt
      301 caaggcgttg gttgattagt aaggctttgt ggcgaatttt gcgagatcgg aacactcgac
      361 cagccggttg agcgttttga gtgtaaaaac cttgccccgg gtggcaaaga gcatcttcct
      421 catgtcctcg cgccggacgc caaaccccct gccggcgatg cacgcaatga cttgatattt
      481 cggctggccg accggtgcgc cggccgtgct gagctcgccc aaatgctgga tgcgggtcac
      541 cttgtcgcgc gcggtgccgt catcttcggt gattttggcc tcgatgacca cacggggagt
      601 aaactcgtcg ggaacgatga aatcaggcgc ctgatcgaag ccgggcaccc tttcggcgcg
      661 cttggtcttt cgatagctga caccggcctt gctcagaacg tcttcaatgg cggactccag
      721 gctgtctccg accaattcgc tcacggagtc tctatggccg gcaaagggac ggccgagaaa
      781 tcgttcgtaa aggagcatgg cgtagggggc ccccatgcgg gcgagtgact tgatggattg
      841 aagcccctct ttggtgtcgg ccttctggag gcgatggatc gtgatgtcat tgggtttcgg
      901 acagccctct tgcaaaagat cgcaggccgc gtccagcagg gcggtcaggc gctcccgggt
      961 cagaccgcgg catttcaagg gcttgagcgg ctgcatccgg atattgcggt cgagagtgcg
     1021 ggcaaagcct tgcgtcaccg cggcgcccct gcgctggctg gccacgtacg cccattcgga
     1081 aggcgtgaag ccgagaatgg agcggatcac tatgaagcag accggcgttg acgcaaccaa
     1141 gtccacgatt tcccgccgag gcagtttgga aaagcccgtc gtgaccttct tcaaatcctc
     1201 gtagcctgct gcgaactggg gatattcgat gaagccttta cccttgggaa gcgtaaggaa
     1261 ctcagaggcg agactggtga caatggccgt aacgaagcgg tcggaatcgg cgaggatttc
     1321 ctggtaatcg gcctcaaaag gatatttcat ctgtcgaggt tcgaaaggct ttatcttctt
     1381 cgttcaattc ccattgttgt tcagccctgg ccctggcata agcatccccc agttcttgca
     1441 gaacggcatc ggccccacca aacttgataa tccaacgccg tacggcggcc atacgatccg
     1501 ccgccttggg cgtagcaacc caagtcgggc gcaggcgcca tacggccagg cggatcagat
     1561 gcccaaagac gacgcagcgc atgtcgccct gggtaggctt catttgccct tgctccaaca
     1621 acctgagatc cccggcggca acgcgggcga gagccgcggg agtgtccgcc agccagcggc
     1681 gggggaattt gcccgtagaa cggcaaacga aaacggtatc cactatggaa gatacggtcc
     1741 cattgatatg aatggaagcc cccatctcgg ccggacaggg cagcgaagcg gagcaggcga
     1801 gcccggagtc gagcatggcg accgctacgg ggaggtacgc gtcgaggctg ttgtgatggt
     1861 aggtgaaggc caatggagcg ccgggattca gggcttttgc catgcggcga aacacccggg
     1921 atatgccttc cgtgaaatgc tccagggtgc gccccatact gacgttgccg gtcagttcgt
     1981 tggtgtggcg cgtcgtaaga ccgtcgaatt caggatgggc agccttgacc agtttacgca
     2041 gccagacgta gcaaaagtcc attagctccg cgtactggac gttctggaag tacggagggt
     2101 cggtcaggat agcgtcgaac ttgcggccgg taaaatcagc ctcggaggca tcggcacacg
     2161 ccagggcgac ttcctttgtc tccggagggt gaaggtcgtt cctctggtcg ccgatccact
     2221 cggtgaggat gggcacccga acctttcgcc tgccttcatg ccggatttca aacgggtggc
     2281 ggcagtacgc tttggccttg gcgaatttgt caacgatgtt aagccagccg ccgctgccca
     2341 ctgccatcgc tttcccggaa atacccagca cgttggattc acactggatc aagccgaccg
     2401 gaaacccgtg cacggagaag acatcaagcg acttgagcgc ctttgtgtcg taccggcaga
     2461 gcaggttctg atagcgaacc aggtcagaca ggttcgtcgc cagtgcgtcg gctattcggg
     2521 agtctttctg ccgcttgatg gcatggcaga ggagttcgag ccccagaagc tgccgcgcat
     2581 taaacatctc ccggtagaat cggtagcccc agcgatggag ccggtcggtt tcatcaccgg
     2641 gaggaatttc ctcctgggga ataaaccggg cctcgccggc agccaggcgg gcggcggctt
     2701 cgtcgtaacg gcggaggtcg gccggagcgg gcttcttgaa gaagcgcccc gagtgcgccg
     2761 gacggcaacg ctcgcaatgg tactcaatgg cgaacatgcg atggggaggc ggtccgccct
     2821 cgggagttgg gtaggcattg acgtggccac aatgcttgca cttgcagcgg ttgcggcggg
     2881 cggtgaactc gacgctaaac cgggtcttgc agtgaggaca ccccggcggg ttcttgcggg
     2941 cggggctgtc atagagctcg ccgcagtcgc ggcaaacaaa gaggttggtg ggatgccgaa
     3001 catccgcggc aacaagatag ttcggaaaca gatccacagg ttggcggcac ttggcgcacg
     3061 gcggtgactt gacccacagg aagtatttga cgtgagcatc ccgggccccg cactcccggc
     3121 actgggtccg atacagatgg ccaatctcgc tttccaacgt gtcccgcagc gtatccgccg
     3181 ccactcggta ggcccgggga ttcagctcgc cgatttcctg gcgcacgatc caccaggcca
     3241 tggggttaat atcaaagccg gtcaccgagc aaccaacccg gttggcctcc agcagggtgg
     3301 tgcccccacc cataaaagga tcgagcacgc ggagatgctt aagcgattgc ccgcggtaaa
     3361 actgctcgcg aagcggccct tctaaaaatt ccgacaggag gagcgcgcga aagagtgtgc
     3421 caggtcggcg cgcaaaccac ttgtgaaccg ccacgactgg acggtagttc tgctggatct
     3481 gcttttcccg caaagccagg tcagccacga agggcacatc aaaatcgcgc tcaatcatcc
     3541 accaaagctg gcagaacccg cctctatgcg cgcccttccg gattgggcgc aacgcgagga
     3601 cgggagaact ggtttacagg actggccgag catggaataa gcttatcaag aattgggcct
     3661 ggcttcaatc tgctttttgg cggcagtttt taccgcttcg ctggcctgct gatgccctca
     3721 gggaaaaagg cgaacccgaa gaactggcca gccgctcacg ccaccaccaa ccccaaaaaa
     3781 ttgcccttgc gcgaagacct cccgcccgcc agcct
//
LOCUS       DAOSRB010000252         3809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_21609_length_3809_cov_5.877730, whole genome shotgun sequence.
ACCESSION   DAOSRB010000252 DAOSRB010000000
VERSION     DAOSRB010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21609_length_3809_cov_5.877730"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLZ74_10370"
     CDS             complement(<1..1275)
                     /locus_tag="PLZ74_10370"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOR98763.1"
                     /translation="MLKRFGYCRVCASSPRVVPGAVEANVAQMVEEARRADAAGVDVL
                     VFPELCVTGYTCADLFFQDTLLDAVERGLAGFLEATRTLAPLCLLGLPVRVSCGVCNA
                     AAVCCAGRLLGVVPKTHLPNTREFSERRWFLSGTGLPDCSLRLCGDEIPFGTDLLFQH
                     EENRLLCVGAEICEDLWSPLPPSGALTLNGATVICNLAAGNELVGKAAYRRALVLQQS
                     ARCHAAYVYAGAGPGESTTDLVFGGHTLIAENGSLLAEGERFERDGTRTLADADLAFL
                     AFERRQNRAFDACAAGVAPVRRVGFVMPERARPVTRRLLRPIAAHPFVPADASERDAR
                     CAEVFALQANGLATRLERAGLRRAVVGLSGGLDSALALLAVCEAFDRVGWERSGILAL
                     TLPGFGTSRRTLDNALALGRALGVTVETIPIAE"
     gene            complement(1318..3489)
                     /locus_tag="PLZ74_10375"
     CDS             complement(1318..3489)
                     /locus_tag="PLZ74_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOR98764.1"
                     /translation="MSITRQKAIESAVQHRVSDEPVLSREPVQACFGSHVFGEDPMRE
                     RLPKDVYQVLRQTIEQGMPLDIRVADVVAAAMSRWAIERGATHYAHVFYPLTGLTAEK
                     HDSFFNPDGKGHLLVDFSGKQLIQGEPDASSFPNGGIRATFEARGYTAWDVTSPAYLM
                     EGRNGITLCIPTAFCSWTGEALDKKVPLLRSMQAVHRQAARVLKLFGHDDPGMINATA
                     GPEQEYFLIDRSLYYARPDLLSAGRTLFGTRPPKGQELEDQYFGAIPARVLAFMTEVD
                     AELYRLGVPVKTRHNEVAPAQYEIAPVFESANLATDHQYLIMQTLQAVAPKYGLACLL
                     HEKPFAGINGSGKHLNWSLNGPKVGNLLNPGETPHENMQFLVFCAAVIRAVDLHAVML
                     RSSVAYAGNDHRLGATEAPPAILSVFLGEQLTDVFEQIAAGGACQSKKAGTLTVGVDT
                     LPQLPRDVTDRNRTSPFAFNGNRFEFRAVGSSQSIAGPQVILNTIVADSLDYIATELE
                     KAPSEPAKFNAAVQKLLQKIIREHGRIIFNGDNYSAEWREEALRRGLPDARTTPEAID
                     AMDVPATYEVFEKCGVLNRREAESRFEIYRRKYIKDVLIEARLCLSMARTMIFPAGVR
                     YQRLLAQTAADLQALGKTTCTTVLDEVNALLAELQQAVRRLEETIEIPPGLNLSQQGR
                     YLVDTVIPLMNALRKVVDELEQICDDGLWPLPTYQEMLFIR"
BASE COUNT          747 a         1296 c         1095 g          671 t
ORIGIN      
        1 ctccgcgatc gggatcgtct ccaccgtgac ccccagtgcg cgaccgagcg ccaaagcgtt
       61 gtccaacgtg cggcgggatg ttccgaagcc cggcagggtc agcgccagaa tgccggagcg
      121 ttcccaaccg acgcgatcga acgcctcgca cactgccagc agcgccagcg cggaatccaa
      181 tcctccggag agccccacca ccgcgcgccg caatccggcg cgctccagcc gcgtggccaa
      241 accgttggcc tgcagcgcga aaacctcggc gcaccgcgcg tcccgctccg acgcatccgc
      301 cgggacaaag gggtgcgcgg caatcggcct cagcaagcgt cgggtcacag gcctcgcgcg
      361 ttcaggcatg acgaaaccca cacgccggac cggcgcgacc cctgccgcac aggcgtcaaa
      421 ggcgcggttc tgccgacgct cgaatgcgag gaacgcgaga tcggcatctg ccagcgtcct
      481 cgtgccatcg cgctcgaaac gctcgccctc cgcgagcaga ctgccgtttt cggcgatcag
      541 cgtgtgtccg ccgaagacca gatccgtcgt ggattcaccg gggcccgccc cggcgtacac
      601 gtatgcggcg tggcagcgcg cggactgctg gagcaccagc gcacgccggt atgcggcctt
      661 gccgaccaac tcattgccgg ccgcgagatt gcagatcacc gtggcgccgt tcagcgtcaa
      721 agccccgctg ggcggcagcg gactccatag atcttcgcag atctctgccc cgacacacaa
      781 cagacggttt tcctcgtgtt gaaacagcag gtccgtccca aacgggatct cgtcaccaca
      841 aagccggagc gaacaatccg gaagtccggt gcccgacaga aaccagcgcc gctcactgaa
      901 ttcgcgcgtg ttgggcagat gcgttttcgg caccacgccc agcagacgcc ccgcacaaca
      961 gactgcggcg gcattgcaga cgccgcaaga cacacgcacc ggcagaccga gcagacatag
     1021 cggcgccaac gtccgcgtcg cctccagaaa ccccgccagt ccgcgttcga ccgcatccaa
     1081 cagcgtgtcc tgaaagaaga ggtccgcgca cgtgtacccc gtcacgcaca actcggggaa
     1141 gaccagcacg tccacacccg ccgcgtccgc tcggcgggcc tcctccacca tctgcgcgac
     1201 gttcgcctcc accgcgccgg gcacaaccct cgggcttgac gcgcagaccc ggcaatatcc
     1261 gaaacgttta agcatcgtcg ctcccccctc atccgcctga gggaacccgg ttccgcctca
     1321 acggatgaac agcatctctt gataggtggg taatggccag agcccatcat cgcaaatctg
     1381 ctccaactcg tccactacct tacgcagcgc gttcatcagc gggatgaccg tatccaccag
     1441 atagcgtccc tgctgcgaga ggttcagccc cggaggaatc tcgatcgtct cctccaggcg
     1501 gcgcaccgcc tgctgcaatt cggcaagcaa cgcgttcacc tcatccagca cggtcgtgca
     1561 ggtcgttttt ccgagcgcct gcagatcggc cgccgtctgc gccagcagcc gctgataccg
     1621 cacccctgcc gggaagatca tggttcgcgc catggagagg cagaggcgcg cctcgatcag
     1681 cacatccttg atgtacttcc tgcggtagat ctcgaaacgg ctctcagcct cgcggcgatt
     1741 gagcacaccg catttttcga acacctcata ggtggccggc acgtccatcg catcgatcgc
     1801 ctcgggggtt gtacgggcat cgggcaaccc gcgccggagc gcctcttcac gccactccgc
     1861 cgagtagttg tcgccattaa agatgatccg accgtgctcg cgtatgattt tctgcagcag
     1921 cttctgcact gcggcattga acttcgcggg ctcgctgggt gccttttcca gttcggtggc
     1981 gatgtaatca agcgaatccg ccacgatcgt gttgaggatc acctgcggtc cggcgatcga
     2041 ctggctggag cccacggcac ggaattcgaa ccggttgccg ttgaacgcaa aaggactggt
     2101 gcggttgcgg tccgtgacgt ccctgggcaa ttgcggcagc gtgtccacac ccacggtcag
     2161 ggttccggct ttcttggact gacacgcgcc gccagccgcg atttgctcga agacatccgt
     2221 gagctgttca cccagaaaaa cggagaggat cgcgggcggg gcttcggtcg cgcccagccg
     2281 gtggtcgttt ccggcgtacg ctacagagct gcgcaacatg accgcatgca gatcgaccgc
     2341 gcggatcacg gcggcgcaga aaaccaggaa ctgcatgttc tcgtgcggcg tctcgcccgg
     2401 attgagcaga ttacccacct tgggcccgtt cagcgaccag ttgaggtgct tacccgaacc
     2461 gttgatcccc gcgaagggct tctcgtggag cagacaggcc aacccgtatt taggggcaac
     2521 cgcctgcagc gtctgcatga tcaggtactg gtgatcggtc gcgaggttgg cgctctcgaa
     2581 caccggcgca atctcatact gcgcgggcgc gacctcattg tgacgcgtct tgaccggaac
     2641 cccaagccgg tataactccg cgtcgacctc ggtcatgaag gccagcacac gcgccgggat
     2701 ggcaccgaaa tactgatcct ccagttcctg acctttgggc ggacgggttc cgaacagggt
     2761 gcgcccggca ctcagcaagt ccggcctggc atagtaaagg ctgcggtcaa tcaggaagta
     2821 ctcctgttcc ggaccagccg ttgcgttaat catgccaggg tcgtcatggc cgaacaattt
     2881 gaggacacgc gccgcttggc ggtggacagc ctgcatcgaa cgcagcagcg gcactttttt
     2941 gtcgagcgcc tcaccggtcc atgagcagaa ggcggtcgga atgcagaggg tgatgccgtt
     3001 gcggccctcc atcaaatacg cggggctggt cacatcccaa gcggtatacc cgcgcgcctc
     3061 aaaggtggcg cggatcccgc cgttggggaa gctcgacgca tccggctcgc cctgaatcaa
     3121 ctgtttgcca ctgaaatcga ccagcagatg ccccttgcca tccggattga aaaagctgtc
     3181 atgcttctcg gcggtcagcc ccgtcagagg ataaaacaca tgcgcgtaat gcgtggcacc
     3241 gcgctcaatg gcccagcggc tcatggccgc ggccaccaca tccgcgacgc ggatgtccag
     3301 cggcatcccc tgctcaatcg tctgccgcaa aacctgatac acgtccttgg gcagtcgctc
     3361 ccgcatcgga tcttcaccga atacatgcga gccgaaacag gcctgcaccg gctcgcgtga
     3421 caaaaccggc tcgtcgctga cccggtgctg aaccgctgac tcaatcgctt tctgtcgcgt
     3481 aatgctcatc gccttctccg ccttgacgtg tcgtgaccga agaaccgaca cggcccttca
     3541 aacagacgcc acgccaacct ccattttgcg caagcacaac atattggact gtttccgacc
     3601 gaaaatcaat cccaactcca cccccaacag gagttgagtt tttacttcgg cgttgaacgc
     3661 tgaaggcttt taagcctgac tatgccccag ttattagcga gtgggccata gataacttca
     3721 acattctcgc ccgtgtccac cttcttgccc gtcttccgca aggaaggtca tgacgaggtt
     3781 gagcatggtt tcgcgctcgg cgggcttgg
//
LOCUS       DAOSRB010000253         3783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_21789_length_3783_cov_6.983101, whole genome shotgun sequence.
ACCESSION   DAOSRB010000253 DAOSRB010000000
VERSION     DAOSRB010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21789_length_3783_cov_6.983101"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..886)
                     /locus_tag="PLZ74_10380"
     CDS             complement(74..886)
                     /locus_tag="PLZ74_10380"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOR98765.1"
                     /translation="MAKKTQQKRSSWDIVQIARHAERPNITDTIRLICDDFVELHGDR
                     LFGDDRGLIGGFATIGGVKIMLIGHRKGRGVEENIEANFGMANPEGYRKAMRLMRLAE
                     KYGLPVVTFVDTPGAYPGSDAEARGQAEAIAHNLEFMSVLKTPIVVVVTGEGGSGGAL
                     GIAVGDRILMLQNAVYSVISPEGCASILWRDGSLAAKAADTLKITSDALKKLGIIDEI
                     IAEPEGGAHTDPAATAAAMKRAVLKHLAELRTIPTEKLLKQRYAKFSAMGQF"
     gene            complement(969..1799)
                     /gene="accD"
                     /locus_tag="PLZ74_10385"
     CDS             complement(969..1799)
                     /gene="accD"
                     /locus_tag="PLZ74_10385"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOR98766.1"
                     /translation="MSWYERPVETPFRVPTEEVWVICPACKAYIGKSAWRAAEKVCPR
                     CNHHARLSCRERVALLTDAGSFKEINADVSFNDPLRFTDASGAYADKARATIAKTGEG
                     ESILTGTAAIESIPVVLGVMDFRFLGGSLGSGTGERIRLAAEHAIATRRPLIIVSASG
                     GARMHEGIVSLMQMAKTCAAIARLKDAGLPYLSVLTDPTTGGVSASYAMVGDLNIAEP
                     KALIGFAGRRVIEQTIKQKLPDDFQTAEYTLAHGFVDCIVPRKELKAFLAKVLRYAGF
                     "
     gene            2016..3377
                     /locus_tag="PLZ74_10390"
     CDS             2016..3377
                     /locus_tag="PLZ74_10390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR98767.1"
                     /translation="MGRRGIRGVAAGCCLALLAASGCLTRDMVYSDLLETREEAYDAW
                     LRIHESGDIPIPSENLTLDEAVRIALLYNKTLQAKIQEREAARGQQLAAYRLALPRVD
                     AAAATESGTDDTESARLTLRQPLFRAGGTTAGLRAARLYAFWSDESVRNTAHSIIRLT
                     TADYYTIRLVENLCQVNRDAVVSAEAHLNDVRIKRVNGVASKYDELRAQVDVANFRAE
                     LIRQENNLVLARNSLLRILGTATDAQFRLADPLTYKPVSMTLEHALQMAYRNRPDLLM
                     AELNIRLQRENVRALRSNYFPEIDLALSAGTNRDRDGDDRWRDTHRAALELNWPLFDG
                     LAREGDLIAAKALLKQREIELQKAEEDVFLDVRQALLNLKNADEYVESQRLNLQHAEE
                     GLRLAMTGYREGVNTEVEIVDARSALTEARGNYYKALYDHCTARLALDHATGLLGQDL
                     ETP"
     gene            3374..>3783
                     /locus_tag="PLZ74_10395"
     CDS             3374..>3783
                     /locus_tag="PLZ74_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOR98768.1"
                     /translation="MKTFAKLLLSLVVGIAIVAGARLAYQAAVRKAHPPASGKGQPSL
                     PVRIAEVRTGNVTNAIRLTGSLEATRVVDVMPKIAGRLERLALEDGTPVLEGLAVSNR
                     QILAVIDHRDILAQMTQAQAAVASARAAVATAKVV"
BASE COUNT          702 a         1264 c         1140 g          677 t
ORIGIN      
        1 tcgcaggtcg acgttcacgc gaacgggaac ggggaaacga tcccggtgcc gtgagggccg
       61 tgcggcgcgg ctcttagaac tgacccatgg cgctgaactt ggcgtagcgc tgtttcagca
      121 gcttttccgt cggaatcgtg cgcagttcgg caagatgctt cagcaccgcg cgtttcatcg
      181 ccgccgcagt cgcggccgga tccgtgtgcg ccccgccctc tggttcggcg atgatctcgt
      241 cgatgattcc caacttcttg agcgcgtcag acgtgatttt cagggtgtcg gcagccttgg
      301 ccgccagcga gccgtcacgc cacaaaatcg aggcgcagcc ctccggggag atcaccgaat
      361 acaccgcgtt ctgcagcatc aggatccggt cgccgaccgc gatgcccagc gcaccgccgc
      421 tgccgccctc gccggtcacc accaccacga tcggtgtctt cagcacggac atgaattcaa
      481 ggttgtgcgc gatcgcctcc gcctggccgc gcgcctccgc gtcgctgccc ggatacgcgc
      541 cgggcgtatc cacaaaggtg accaccggca gcccgtattt ctcggcgagg cgcatcaggc
      601 gcatcgcctt gcggtaccct tcgggattgg ccatcccgaa gttggcctcg atgttctcct
      661 cgaccccgcg acctttgcgg tggccgatga gcatgatctt cacgccgccg atcgtggcga
      721 acccgccgat caggccgcgg tcgtcgccga acagccggtc accgtgaagc tcgacgaaat
      781 cgtcacagat caggcggatc gtgtccgtga tattcggacg ctcggcatgg cgcgcgatct
      841 gcacgatatc ccaggatgaa cgtttctgct gtgttttctt agccatatgc cctaccgatt
      901 aacaagttag ctggtctcgg gccgctgcgc gccgtccgtc gcgtcgggag gcgcgtccgc
      961 ggcgcgtgtc aaaagcccgc gtaacggagc acttttgcca gaaaggcctt cagctccttg
     1021 cgcggcacga tgcaatccac aaagccgtgg gcgagggtgt actccgccgt ctggaaatca
     1081 tccggcagtt tctgcttgat cgtctgctcg atcacgcggc gtcccgcgaa cccgatcagg
     1141 gccttgggct ccgcgatgtt cagatcgccg accatcgcgt aactggctga aaccccgccg
     1201 gtcgtcggat cggtcagcac cgaaagatac ggcaggcccg catccttcag ccgcgcgatt
     1261 gccgcgcacg tcttggccat ctgcatcagc gagacgatcc cctcgtgcat gcgcgcgccg
     1321 ccggatgcgg aaacgatgat cagcgggcgc cgggtcgcga ttgcatgctc tgcggccagc
     1381 cggatgcgct cgccggtgcc gctgcccaga ctgcctccca aaaaacggaa atccatgacg
     1441 cccagcacca ccggaatcga ttcgatcgcc gccgtgccgg tgaggatcga ctcgccctcg
     1501 ccggtcttgg cgatcgtggc acgggccttg tccgcatagg caccgctcgc gtcggtgaac
     1561 cgtaacgggt cgttgaaact gacgtcggca ttgatctcct tgaaactgcc cgcatcggtc
     1621 agcaacgcca cccgctcgcg gcacgacagc cgcgcgtgat gattgcaccg cggacagacc
     1681 ttctccgccg cgcgccacgc actctttccg atgtacgcct tgcaggcggg gcatattacc
     1741 catacctctt cggtcggaac acggaacggc gtctctaccg gtcgctcata ccatgacatt
     1801 ttcgcttact ccttcactca cccggacgct ctcccgccaa gccaaccccc ggcggcacgt
     1861 cccatacggc ctgttcccgc tttgatcaag gtgacgataa cattgttttt atcattctga
     1921 caagaccggc cctatttttt tattatacac tccacacaaa accgtgtaac gtaatggggt
     1981 ttcaatgggg aagcgaacgt ttcggaacgg gggatatggg acgaagaggg atcaggggag
     2041 ttgcagccgg gtgttgcctg gccctgctgg cggcatcagg gtgtctaacg cgtgatatgg
     2101 tgtacagcga tctgctggag acgcgcgaag aggcttacga cgcatggtta cggatacacg
     2161 agagcggcga catcccgatc ccatccgaaa acctgaccct ggacgaggcg gtgcgtattg
     2221 cgctgctgta caataaaaca ctccaggcca agattcagga gcgcgaggcc gcacgcggcc
     2281 agcagctcgc cgcctaccgg ctcgccctgc cccgggtgga cgccgcagcc gccaccgaat
     2341 ccggcaccga tgacaccgag agtgcccgcc tcaccctgcg ccagccgctc ttccgggccg
     2401 gcggcaccac cgcgggactg cgcgccgcgc gcctctatgc cttttggtcg gatgaaagcg
     2461 tgcgcaacac cgcccattcc atcatccgcc tcaccaccgc cgattattac acgatccggc
     2521 tcgtcgaaaa cctctgccaa gtcaaccgcg acgccgtggt ctcggctgag gcgcacctga
     2581 atgacgtgcg tatcaaacgc gtcaacggcg tggcctcgaa atacgatgag ctgcgggccc
     2641 aggtggatgt ggcgaacttc cgcgctgaac tcatccgtca ggagaacaac ctcgtgctgg
     2701 cccggaacag tctcctgcgc atactgggca ccgcgaccga cgcgcagttc cggctcgcgg
     2761 acccgttgac gtacaaaccg gtgagcatga cgctggaaca tgccctgcag atggcgtatc
     2821 ggaatcggcc ggacctgctc atggcggaac tcaacatccg cttgcagcgc gagaacgtgc
     2881 gggccttgcg cagcaactat tttccggaga tcgaccttgc cctgagcgcg ggcaccaacc
     2941 gtgaccgcga cggcgatgac cgctggcgcg acacccaccg cgccgccctg gaactgaact
     3001 ggccgctttt cgacggcctc gcgcgggaag gggatctcat cgcagccaag gccctgttga
     3061 aacagcggga gatcgaactc caaaaagcgg aagaggatgt gtttctggat gtccggcagg
     3121 cgctgctcaa tctgaaaaac gccgatgagt atgtcgaatc gcagcggctt aacctgcaac
     3181 atgccgagga ggggctgcgt ctggccatga ccggttaccg cgaaggggtc aacaccgagg
     3241 tggagattgt ggacgcgcgc tctgccttga ccgaggcgcg aggcaattat tacaaggcac
     3301 tgtatgacca ctgtacggcg cggctcgcgc tcgaccacgc caccggtctg ctgggccaag
     3361 atttggagac gccatgaaaa cgtttgcgaa actccttctc tctctcgttg tcggcatcgc
     3421 catcgtcgcg ggtgcgcgtc tggcctatca ggccgcggtg cgcaaagccc acccgcccgc
     3481 cagtggaaag ggacagccgt cgcttcccgt gcggatcgcg gaggtccgca ccggaaacgt
     3541 gaccaacgcc atccgcctga ccggctcgct ggaggccacg cgagtcgtgg atgtgatgcc
     3601 gaagatcgcg ggacggctgg aacgcctcgc gctggaggac ggcacgccgg tgctcgaagg
     3661 gctcgccgtc tccaaccgcc agatcctcgc cgtcatcgat caccgtgaca tcctcgccca
     3721 gatgacgcag gcccaagccg cggtcgcaag cgcgcgcgcc gccgtggcca ccgccaaagt
     3781 ggt
//
LOCUS       DAOSRB010000254         3733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_22161_length_3733_cov_5.803970, whole genome shotgun sequence.
ACCESSION   DAOSRB010000254 DAOSRB010000000
VERSION     DAOSRB010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22161_length_3733_cov_5.803970"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..>3733)
                     /locus_tag="PLZ74_10400"
     CDS             complement(218..>3733)
                     /locus_tag="PLZ74_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98769.1"
                     /translation="AFPEFRSRVAYLDIETDGAQTIRLAQATDIGTVFGHADQTISSP
                     MRSGPYRAFKSINVANPNEFAGQWTSGDSGALWNVTPTADFTPMFASFDAKMRPAMYA
                     SSGCVRFGTITGGGMLFVHGGGNAQVDDVALLTRGRTRLRVPDGTTLSLGMASTFPSN
                     AVTGAYVRFDAARADTLTLENGADGRTHVTEWRDAEGGDVKAIVTTRAAAPYLSTVKS
                     IRGTPLVDFGAYRSVSHEDTWMNFDAFEAAAGPCGGLRFDETSEAREIFLVFEETIPS
                     NVYPFALGGWDSYHLHRGSNGTLFHHGHAASSVRTGSVTLDGAPFTPLGQANFSRLHL
                     VSLSTATGIKVGTLAIDRDQRFGGVRIAEAIIYTRALSEAERRQNINYLLFKWRPESF
                     PEHDLDLLLIPGNGTVRVPTNESVTVDSLYLPRYRTLTKTGDGDLRVHDLIEDDISFT
                     VRGGRLRVSASQDVVTDTAPAESPLFHLDASDAASLVETNVIDGTGRRYVARWNDARG
                     VANYADHDAGDRTFPYIVEGAANGRDVLDFGEGWIKEWGPTKTIADWGDVSCGDTARL
                     ICSKAFNANAGFIVLRTKKVNGYSETDHAYLNPPMFGSSGYDFTRWGSGDLVTFYNSA
                     AVQTALWEFDGKPFEPVDRTAPITTNEFLVVSFCSESRAMVNRIADDRLNAVGGVQIA
                     EMLLYDRPLSPWERKCTQAYLMKKWKNAVHPALKSPVSVRALAFADGATAEFETERDV
                     VIGSVNTGGAKLTVKGRGHVTLPPCYSTAAGLRVDGGSVTIPADDPFADALYHFDASV
                     TDSLDCEYVDNGNGTVTTNVVRWHDVRGNAVVATAFTNTATCRAYPVLRTVTTRNGRS
                     MPVIDFGELSKAAAKIYKDSSAGMAISIPGWDGLMGEGHVVWCDAHGGCGDRFIFPDS
                     VYYSAHRGENASGQLFCAYTQNPYSQYIKDGYVAVDGVPRAYNYNMDDQAFHVVSLAP
                     SNAIPNRLIGADRNNQRGGGSYQGELIVFASPLPAERREYLQRFLAWKWFGEGTSPQH
                     PFCSLAALELVHGGTLTLTGTMPYACSSLKVEGVATMNGGLTLEEGGMLDFAVNDTND
                     VACLTVNGTFALPSSGTLKVQVGAGFAGATGSFPLITATELLGNVTGWIRDFEKEADV
                     GVRVRVSDNTLLLDVLPSGMIIILR"
BASE COUNT          741 a         1212 c         1070 g          710 t
ORIGIN      
        1 ggcggacctc ctcccccttc tctcttctcc aaggtgtggc ggttagtata gccgctcacc
       61 cctcgacctt caatcttgaa tctttcgacg cttggcttca gggcagggct tcttctttta
      121 ggctgtttgg tttgaaaaca ggttgcatga actttactgc gaacaaaaaa atcgcaaata
      181 tatccgcagc tagacacacc ccgtcataca ccagaggtca gcgcaggatg atgatcatgc
      241 cggaaggcag gacatcgagc agcagcgtgt tatcggacac gcgcacccgc acaccgacat
      301 ccgcctcctt ttcgaaatcg cgtatccagc cggtgacatt ccctaaaagt tccgtcgccg
      361 tgatgagcgg gaacgatcct gtcgcgcccg caaagccggc tcctacctgc accttcagcg
      421 tacccgacga cggcaacgcg aacgtgccgt tcaccgtcag acaggccacg tcgttcgtgt
      481 cattgacggc aaagtcgagc atgccccctt cctccagcgt cagtccaccg ttcatcgtgg
      541 ccaccccctc aaccttgagc gaagaacacg catacggcat tgtacccgtc agcgtgagcg
      601 taccaccgtg cacgagttcc agcgcggcga gcgagcagaa cgggtgctgg ggactcgtcc
      661 cctcgccgaa ccacttccat gcaaggaacc tttgcagata ctcacgtcgt tccgcaggca
      721 acggggatgc aaacacgatc agctcgccct gataactgcc gccgccgcgc tggttgttac
      781 ggtcagcgcc gatcagacgg ttcgggatcg cgttcgacgg cgcgagcgac acgacgtgga
      841 acgcctggtc gtccatattg tagttgtatg cgcgcggcac gccatcgacc gccacgtagc
      901 cgtccttgat gtactgcgaa tagggatttt gggtataggc gcagaacaac tgcccgcttg
      961 cgttttcacc gcgatgggcg gaataataca ccgaatccgg gaagatgaac cggtcgccgc
     1021 acccgccgtg cgcatcgcac cagacgacat gcccttcgcc catgagcccg tcccaccccg
     1081 gaatactgat cgccatgcct gcggaactgt ccttgtagat cttggccgca gccttgctaa
     1141 gctcgccgaa gtcgatcacc ggcatggaac gtccgttacg cgtcgtgacg gtccgcagca
     1201 cggggtacgc ccgacaggtc gccgtgtttg tgaacgccgt cgcgacgacg gcattcccgc
     1261 gcacgtcgtg ccaccggacg acgttcgttg tcacggtgcc gttgccgttg tcgacatact
     1321 cgcaatcgag cgaatccgtg acggacgcgt caaagtggta gagcgcgtcc gcgaacggat
     1381 cgtctgcggg gattgtcacc gacccgccgt cgacgcgcaa ccccgctgca gtcgaatagc
     1441 agggcggcag tgtcacatgg ccacgtccct tcacagtcag cttcgctccg cctgtgttga
     1501 cggaaccgat cacaacatcg cgttccgtct cgaactccgc cgtcgcaccg tcggcaaacg
     1561 cgagcgcgcg cacggagacg ggcgacttca gcgcgggatg caccgcattc ttccacttct
     1621 tcatcagata cgcctgagta catttccgct cccacggcga aagcggacga tcatagagaa
     1681 gcatttcggc gatctgcaca ccaccaacag cgttgagacg gtcatcggcg atgcggttca
     1741 ccatagcccg cgactcggaa cagaaactca ccacgagaaa ctcgttggtc gtgatcggtg
     1801 ccgtcctgtc caccggctcg aacggcttgc cgtcgaactc ccacaacgca gtctgcacgg
     1861 ccgccgagtt gtagaaggtg acgagatcgc cgctgcccca gcgcgtgaaa tcatacccgc
     1921 tgctcccgaa catcggcgga ttgagatagg catggtctgt ctcgctgtaa ccgttcacct
     1981 tctttgtgcg tagcacgatg aatccggcat tcgcgttgaa tgccttggag cagatgaggc
     2041 gcgccgtgtc accacagctt acatcgcccc agtcggcgat cgtcttcgtc ggtccccatt
     2101 ccttgatcca gccctcgccg aaatcaagca catcccgccc gttcgccgcg ccttcgacaa
     2161 tatacgggaa cgtcctgtcc cccgcgtcgt gatcagcgta gttcgcaacg ccgcgcgcat
     2221 cattccagcg cgccacgtaa cgacgtccgg ttccgtcgat cacgttcgtc tcgacaagag
     2281 acgccgcatc ggacgcatca agatggaaga gcggagattc cgcaggcgcg gtgtctgtca
     2341 ccacatcctg cgacgcggaa acacggagac gtccaccacg cacggtgaac gagatgtcgt
     2401 cctcgatcag atcgtgcaca cgcaggtctc cgtcacccgt cttcgtcaac gtccggtatc
     2461 tcggcagata gagagaatct accgtgaccg actcgttagt aggcacccgc acggtgccgt
     2521 tgccgggaat gagcagcagg tcgaggtcat gctccggaaa cgattcagga cgccacttga
     2581 aaagcaggta gttgatgttc tgcctgcgtt ccgcttcaga cagcgcacgc gtgtagataa
     2641 tcgcctcggc gattcgcaca ccgccgaacc gctgatcgcg atcgattgcg agagtgccga
     2701 ccttgatccc tgttgccgtc gagagcgaca cgaggtgcag acgcgagaag ttggcttgcc
     2761 ccagcggcgt gaacggggcg ccatcgagcg tgacactgcc cgtacggaca gaggaagctg
     2821 catgtccgtg gtgaaagagc gttccattgg atccccggtg gagatggtag gagtcccacc
     2881 cgccgagcgc aaacggataa acattgctcg gaattgtctc ctcgaagacc agaaagatct
     2941 ctcgtgcctc actcgtctca tcgaagcgga gtccgccaca gggccctgcc gccgcctcga
     3001 atgcgtcgaa attcatccat gtatcttcgt ggctgacact tcgatacgcg ccgaagtcga
     3061 cgagcggcgt tccgcgaatg gacttgaccg tgctcagata cggcgccgcc gcgcgcgtgg
     3121 tgacaatcgc cttcacgtct ccgccctccg cgtcgcgcca ctccgtcacg tgtgtgcggc
     3181 cgtctgcgcc gttctccagc gtcagcgtat cggcgcgcgc ggcgtcgaaa cgcacgtagg
     3241 caccggtgac ggcgttactc gggaacgtgc tggccattcc gagcgagagt gtcgtgccgt
     3301 cgggcacgcg aagccgtgtg cggccacgcg tgaggagcgc cacgtcgtct acctgcgcgt
     3361 tgcctccgcc atgcacaaac aacatccctc cgccggtgat cgtgccgaag cggacgcatc
     3421 ccgaactcgc atacatggcg ggacgcatct tcgcgtcgaa ggaggcgaac atcggtgtga
     3481 agtcggccgt cggcgtgaca ttccagagcg ctccggaatc cccgctcgtc cactgtcccg
     3541 caaattcatt gggattggcg acgttaatgc tcttgaacgc cctataaggc cccgaacgca
     3601 tcggagagga gatcgtctgg tcggcgtgtc cgaagacggt gccgatgtca gtcgcctgtg
     3661 caagacggat tgtctgtgcg ccgtcggtct cgatgtccag gtaggccacg cgcgagcgga
     3721 actccgggaa cgc
//
LOCUS       DAOSRB010000255         3729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_22188_length_3729_cov_4.354382, whole genome shotgun sequence.
ACCESSION   DAOSRB010000255 DAOSRB010000000
VERSION     DAOSRB010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22188_length_3729_cov_4.354382"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="PLZ74_10405"
     CDS             <1..1318
                     /locus_tag="PLZ74_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOR98770.1"
                     /translation="AIRQARVLPGFGRRNEALGEWARLYRRLPRTLLAGGWEGASFDG
                     HAGAVKSIRLSGDGRYAVSGGADGMVKIWNMSSGKRLRTLKVRHGAVHSVACSVEGRF
                     LVAGGEDGLIQMWDAVQGDQIQTFEGFTGTVETLALSLDGRFLVSAGLDIWIWDVATA
                     EPLRTLEGHSSDIVSLCLSPDSLSLLSGSSDQNVALWDIASGNSLMTLAGHAGGVRTV
                     SLSEDGRFALSGSGNMWGRPGELFLWNLETGECLHSFEGHASSVNAACLTTDCRYAVS
                     GSSDRTVKLWETSSGECVRVFQGHADAVEALGMSRDGRYVLSGSADGKMIQWILDWEL
                     EEREREDWDEGARPYLEAFLTLHTPYAADVSQEGPQSEEEATLALTHRGKPEWNDEDF
                     RRLMYTLGCAGYGWLTQEGVQQQLEKISARRKWSSFFVGKWGKRTR"
     gene            complement(1431..>3729)
                     /locus_tag="PLZ74_10410"
     CDS             complement(1431..>3729)
                     /locus_tag="PLZ74_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOR98771.1"
                     /translation="YQPEKYAVRIRVRVIARPVKTADTINNEAVFQKQIDVYPAAEDV
                     IRDDLGNGKRSCGGVGSPTFYDINGDGVDEMLLPSEDGFIHAYTDVATGTELPGWPVH
                     TSLYPGIRTTGVNAYTKGKVMKTVYSSILFGSVAVADLFDKGDLQVLAADLEGRLYAW
                     EVTGKARAKFPVTVDYNLSKQPPCGPATIPNCDDYTSPPRRDVYNARDWAFNSAPAIG
                     DLDPTFNGLEIVIGSSDGHIYAFRNNGTLVPGWPVLLRDPAKVATMDPVSRAITYRSD
                     ANQEIGTKVVVTPSLGDINGDGRLDVVVGVNEQYSEPMNATANDSLLEQVVSLIGAPG
                     NGRIYALYNTGSLTPETPASSATPHLQDQAYMPGWPVKIALINLDLLPYVGLGTNGQA
                     ALADFDGDGKLEIVCGTVMGPAYILRSDGSSFLGKDGSVYKTMNKGASEFGYYARNAA
                     NPTTDGTSFTALGGFAIGNMTRGPIISGPRPANWPVEHLTIASSAGGINRTLDILLEG
                     KQLGSQDQLALWSPDGKYEPVAPLLVNDLQFFSSPTIADINGDGWAEVIQSTAASDTV
                     AACYFNTNDTATRYYTGGWSMSSVALGNAPMGARSDGKLRLVTVTREGYLRLYPTPVV
                     AGTPEAARALSEWPEFGHDPRNTGNYHVDAVRPYPLRDIEVTPLPGKKLQVKVTATGD
                     DRYEGAAARYEVRYLAGTVAAPSWLSGKVLTLAANTPSPAGQQDTLVTTKVLPAVAAY
                     TIVVRAFDEAGNGSVVAKAYVTSLP"
BASE COUNT          646 a          992 c         1297 g          794 t
ORIGIN      
        1 cgccattcgt caagcgcgcg ttctgcccgg atttggtcgt cgcaacgagg cgctgggcga
       61 atgggcgcgt ctttaccgcc ggctgccgcg caccctgctg gcgggcggat gggaaggcgc
      121 ttcttttgac ggtcatgccg gagcggtcaa atcgatccgg ctaagcggcg atgggcggta
      181 tgccgtctcc ggcggcgccg acggcatggt caaaatctgg aatatgtcat cgggaaaacg
      241 cctgcggacg cttaaggtgc ggcatggggc ggtgcattcg gtggcctgca gcgtggaggg
      301 ccgtttcctc gttgcggggg gggaagacgg cctcatccag atgtgggatg cagtgcaagg
      361 cgatcaaatt cagactttcg aagggttcac gggcacggtc gagacgttgg ctttgagtct
      421 tgacggccgt ttcctggttt ccgccggttt ggatatttgg atatgggacg ttgcgacggc
      481 ggaaccgctg cgtacgctcg aagggcattc gtcggacatc gtttccttgt gtctcagtcc
      541 cgactcactc tcgttgcttt cgggcagttc ggatcaaaac gtggcgcttt gggatatcgc
      601 ctccggcaat tcactgatga ccttggcggg ccacgcgggg ggcgtgcgca cggtatcctt
      661 gagcgaagac ggacgtttcg ccttgtcagg cagcggaaac atgtggggga ggcccggaga
      721 gcttttcttg tggaacctgg agacgggaga atgtctgcac tcgtttgagg gacatgccag
      781 ttcggtcaat gccgcctgct tgacgacgga ttgccggtat gccgtgtcgg gcagttcgga
      841 ccgcacggtc aagctttggg agacttcttc gggggaatgc gtgcgcgtgt tccagggaca
      901 tgcggacgcg gtggaagcct tgggcatgtc tcgagatgga cggtatgtgc tttcgggcag
      961 tgcggacgga aagatgatcc agtggattct cgattgggaa ctcgaggagc gggaacggga
     1021 agattgggat gagggcgccc ggccgtatct cgaggcgttt ttgacgttgc atacgccgta
     1081 tgccgcggac gtgagccagg agggaccgca gtccgaagag gaagcgacct tggcgttgac
     1141 gcatcgcggc aaaccggaat ggaacgacga ggatttccgg cggcttatgt atacgttggg
     1201 gtgtgcggga tacgggtggc tgacgcagga aggcgtgcag cagcagttgg agaaaatctc
     1261 cgctcggcgc aagtggagct cttttttcgt cggcaaatgg ggaaagcgca cccgttaacg
     1321 catttcacgt cgctgcttct gctcgctccc ccccctttcc acttttcaca tcacgcaaag
     1381 agcccccgca acgattacgt tgcgggggct cttgtgtgta cgtcttggcg ttacggcaac
     1441 gaggtcacat atgccttggc tacgacggat ccgtttccgg cttcgtcaaa ggcgcggacg
     1501 acgatggtgt aggccgcaac cgccgggaga accttggtgg tgaccagggt atcctgttgt
     1561 cccgcaggag atggcgtgtt ggcggccaag gttagcacct tgcccgagag ccaactgggg
     1621 gcggcaacgg tgccggctag gtagcgcact tcgtagcgcg ctgccgcgcc ttcgtagcga
     1681 tcgtcgccgg tcgcagtcac cttcacttga agcttctttc cgggaagagg cgtaacttcg
     1741 atatctcgca ggggataggg ccgcacggca tcgacgtggt aattgcccgt gttgcgcgga
     1801 tcatgaccga attccggcca ttccgatagg gcccgcgccg cctcgggggt tcccgccacg
     1861 acaggagtcg gatacagacg taggtagccc tcgcgcgtaa cggtgaccag ccgcagtttc
     1921 ccgtcgctgc gggctcccat gggagcgttg cccaatgcca ccgaactcat cgaccagccg
     1981 cccgtgtagt agcgggtggc cgtgtcgttg gtgttgaagt aacacgcggc cacggtgtcg
     2041 ctggcggcgg tggattggat aacttcggcc cagccatcgc cgttgatgtc cgcgatggtc
     2101 ggcgaactga agaattgaag gtcgttcacc aacaaggggg cgacgggctc gtattttccg
     2161 tcgggactcc agagggcgag ttggtcttga ctgcccagct gcttgccttc caacaggata
     2221 tccaaggtgc ggttgatgcc gcccgcggaa ctggcgatgg tcaggtgttc gacgggccag
     2281 ttggccggac gcggccccga gataattgga ccgcgcgtca tgttgccgat ggcgaaaccg
     2341 ccgagcgccg tgaagctggt gccgtcggtg gttggattgg cggcgttgcg ggcgtagtag
     2401 ccgaattcgc tcgcgccttt gttcatggtt ttgtaaacgc ttccgtcttt gccgaggaag
     2461 gaggaaccgt cgctgcgcag gatataggca ggccccatga ctgtgccgca gacgatctcc
     2521 agttttccgt cgccgtcaaa atcggccaag gcggcttggc cgttggtgcc caagccgacg
     2581 tagggcaaca ggtcgaggtt gatcagagca atcttgaccg gccatccggg catataggcc
     2641 tggtcctgca aatgcggcgt tgcgctgctg gcgggggtct cgggagtgag agagcccgtg
     2701 ttgtacaacg catagatgcg gccattgccc ggggcgccga tgagactgac gacctgttcc
     2761 aagagggaat cgttggccgt ggcgttcatg ggctcgctgt actgttcgtt gacgcccacg
     2821 actacgtcca atcttccgtc gccgttgatg tcgcccaatg agggcgtaac cacgactttg
     2881 gttccaatct cttgattggc gtcggaccta taggtgatgg cgcgggagac ggggtccatg
     2941 gtcgcgacct tggcagggtc gcgcaagagc acgggccacc cgggaacgag agtgccattg
     3001 ttgcggaacg catagatgtg tccgtcgctg ctgccgatga cgatttcgag gccgttgaaa
     3061 gtgggatcga gatcgccgat ggccggagcg ctgttgaagg cccagtcacg ggcgttgtag
     3121 acgtcgcggc gcggaggcga tgtgtaatcg tcgcagttgg gaatggtcgc cggaccgcat
     3181 ggcggttgtt tcgagagatt gtagtccacc gtgacgggga atttggcgcg ggcctttccg
     3241 gtgacttccc aggcatagag acgcccttcg aggtcggcgg ccaggacctg gaggtcgcct
     3301 ttgtcgaaca ggtcggcgac cgcgacgctg ccaaacagga tggagctgta cacggttttc
     3361 atgaccttgc ccttcgtgta ggcattgacg ccggtggtgc ggatgccggg atagagactg
     3421 gtgtggacgg gccagcccgg cagttcggtc cccgtggcca cgtcggtgta agcgtgaatg
     3481 aagccgtctt cgctgggaag caacatttca tcgacgccgt cgccgttgat gtcatagaag
     3541 gtgggcgaac cgacgccgcc gcaggaacgt tttccgttgc cgagatcgtc tcggatgacg
     3601 tcttcggcgg cggggtacac gtcgatttgt ttctggaata cggcctcgtt gttgatcgtg
     3661 tcagcggtct tgacgggccg ggctatgacg cgtacgcgaa tgcggacggc gtatttctcg
     3721 ggttggtag
//
LOCUS       DAOSRB010000256         3728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_22199_length_3728_cov_7.497414, whole genome shotgun sequence.
ACCESSION   DAOSRB010000256 DAOSRB010000000
VERSION     DAOSRB010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22199_length_3728_cov_7.497414"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLZ74_10415"
     CDS             complement(<1..256)
                     /locus_tag="PLZ74_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98772.1"
                     /translation="MAIIVLLTTSVAWAEQPLLPSKLREGWRENRNNMLWWHPYDTFD
                     GTNAAFELAHGLPPGGAVRCFGEQLLSELAKPDGGDSNRVA"
     gene            complement(287..3058)
                     /locus_tag="PLZ74_10420"
     CDS             complement(287..3058)
                     /locus_tag="PLZ74_10420"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98773.1"
                     /translation="MSTPEMRWQTLLVLALYSAWIAAVCHERRIFKRLASAIAGAWAS
                     WPPALRILMAGLLLACVKDGRCTKPGGGTNSPAATAQQPAAREGQAAGADTGGAAGAP
                     AAAAPGQAGQTPDNAPPAPAPALPALADGTNCWWVAEGRGPADADADGMPDAWERVFG
                     LDPLDPSDAAGDLDRDGLTALEEFLNRTHPRRRDTDGDGMPDAWEAARRPSLCPWLPD
                     DGGDADGDGLDNFREMALGADPLDPDTNGDGRPDGGEAAAGVDPALDPPDWAAYGTAL
                     FSVRVDGVRAARRAGVSVGHITHSGVPERTYALAAGYRYPLTVVDLDPSNTVPCAGTV
                     RVTHDNATFMHGFTNEFPVTFPLSANAPACPPGAEMTVAGIDLDIPDIIYPAPGNSAS
                     FPVSADFVPDGEGIAGQPQWSVSGGSATPASGSMGTWVSVTFPPPARQAQGGPTPTGE
                     GVTTNNPPSVEVTVGDRTVTRTLEQNTPDDPDPDDPDPWDGRRFIARSCSDAEPVALT
                     YALAHSSNAVTLAWHGHQEDAVLEWTIGGDGPRFLKDGQEVSRVTRDLSVSVLPRGEV
                     ENFTIRAKALTQLGTVITRQTELRVVTIVAEPVRDFYPGGGGPLVNPSGFRVGDEATF
                     KFEFSDNVDGSHVCWSKIGDAAVWSGTPVETGRGDIVLRGNQPGEGTLKVSVENGCGI
                     PDPGLNFKVFEVSAPIPVHVGILCDTNGQESVTLDAVNTKIGLASNIFRQVGIGFRLA
                     SVTWITNQGFYVSDPLRTPGYLYTNDLIRAQIQQNDGLEVYFCPWSMVQASGAQGVWA
                     EEGILIAKDKADAVVAHELGHAFGWEDICGEKRVGHLNTAYTVSVEGPVSQARLPYDW
                     NNGPGPEEYYERGLQQMEVIDRLLMSSSTLGMDIPSGRVFGLPREGVSPRLTHVGERD
                     MVTRTPVSQ"
BASE COUNT          578 a         1273 c         1181 g          696 t
ORIGIN      
        1 ccgccacccg gttcgagtcc ccgccgtccg gtttggccag ctcggacagc agttgttcgc
       61 cgaaacaccg gacggctccc cccggtggca agccgtgtgc caattcgaac gcggcgttgg
      121 tgccgtcgaa cgtgtcgtac gggtgccacc acagcatgtt gttccggttt tcgcgccagc
      181 cttcccgaag cttggaaggc agcagaggct gttccgccca tgccactgac gtggtcagca
      241 gaacaatgat tgccatgttt ctggtcttca tccttcatct cctctcttat tgggaaacgg
      301 gggttcgcgt aaccatgtcg cgttccccga catgggttaa tctgggggaa acaccttctc
      361 taggtagccc gaagacccgt ccggacggga tgtccatacc gagcgtgcta ctcgacatga
      421 gcagacgatc gatgacctcc atctgttgca acccgcgctc gtaatactct tccggccccg
      481 gaccattgtt ccagtcgtaa ggcaaacgtg cctgtgaaac agggccttca acgcttactg
      541 tgtaagcggt gttgaggtgt ccaacccgtt tctccccgca aatatcctcc cacccgaaag
      601 cgtgccccaa ctcatgcgcc acgacggcgt ccgccttgtc cttcgcgatc aggatgcctt
      661 cctctgccca gacgccctgc gcccctgagg cctgaaccat gctccacggg cagaaataga
      721 cctccagccc gtcgttctgc tggatctggg cacggatcag gtcgttggtg taaaggtagc
      781 ccggcgtgcg tagaggatcg gaaacgtaaa acccttggtt cgtgatccag gtcacggacg
      841 cgaggcggaa gccgatgccg acctggcgga aaatgttcga ggccaggccg atcttggtgt
      901 tcacggcgtc cagcgtgacg ctttcctgcc cgttcgtgtc gcagaggatg ccgacgtgaa
      961 ccgggatcgg ggcggacacc tcgaagacct tgaaattcag tccggggtcg gggataccgc
     1021 aaccgttctc caccgacacc ttgagtgtcc cttcgcccgg ctggttgccg cgcaacacga
     1081 tatcccccct gccggtctcg acgggggttc ccgaccagac cgccgcgtcg ccgatctttg
     1141 accaacacac atggctgccg tccacattgt cagagaactc gaacttgaac gtcgcctcgt
     1201 ccccgacgcg gaacccgctg gggttgacca agggtccgcc cccgccggga tagaaatcgc
     1261 ggaccggctc ggcaacgatg gtgaccacgc gcagctcggt ctgccgcgtg atgaccgtgc
     1321 ccagttgcgt cagggccttc gccctgatcg tgaagttttc tacctctccg cgcggcagga
     1381 ctgagaccga gaggtcccgt gtcaccctgg acacctcctg cccgtccttg agaaagcgcg
     1441 gcccgtcccc gccgatggtc cactccagca ccgcgtcctc ctgatgcccg tgccacgcga
     1501 gcgtcacggc attgctgctg tgcgcgagcg cgtaggtcag cgccaccggt tccgcgtcgc
     1561 tgcaagagcg ggcgatgaaa cgccggccgt cccaggggtc ggggtcgtcg gggtcgggat
     1621 cgtccggcgt gttctgttct aaagttcgcg tgacggttct gtccccaacc gtcacctcga
     1681 ccgacggcgg gttgttggtc gtcacgcctt cgcccgtcgg cgtcggtccc ccctgggcct
     1741 gccgcgcggg cggggggaag gtcacgctca cccatgtccc catgctgccg cttgccggtg
     1801 tggcactccc gccgctgacg gaccactgcg gctgccccgc gatcccctcg ccgtcgggca
     1861 cgaagtcggc cgacacggga aaggacgcgc tgttgcccgg ggcgggataa atgatgtcgg
     1921 ggatgtcgag gtcgatgccg gccacggtca tctccgcgcc gggcgggcag gcgggggcgt
     1981 tggcggagag cgggaacgtc accgggaact cgttggtgaa gccgtgcatg aacgtggcgt
     2041 tgtcgtgggt gacgcgcacc gtgcccgcgc aggggacggt gttgctcggg tcgaggtcga
     2101 caaccgtcag cgggtagcgg tatcccgccg cgagggcgta cgtccgctcc ggcacgcccg
     2161 agtgcgtgat gtgcccgacg gacacgcccg cgcggcgcgc ggcccgcacc ccgtcaaccc
     2221 gcacggagaa cagggccgtc ccgtaggccg cccagtcggg cgggtcgagc gcggggtcca
     2281 cgcccgcggc cgcctccccg ccgtcggggc gcccgtcgcc gttggtgtcg gggtccagcg
     2341 ggtccgcccc cagcgccatc tcgcggaaat tgtccagccc gtcgccgtcc gcgtcccccc
     2401 cgtcgtccgg cagccacggg cagagggagg gcctgcgcgc cgcctcccac gcgtcgggca
     2461 tgccgtcgcc gtccgtgtcc cggcggcgcg ggtgggtgcg gttcaggaac tcttccagcg
     2521 cggtcagccc gtcgcggtcg aggtcgccgg cggcgtcgga ggggtcgagc gggtcaagcc
     2581 cgaagacgcg ctcccacgcg tcgggcatgc cgtccgcgtc cgcgtccgcc gggccgcgcc
     2641 cctcggcgac ccaccagcag ttggtgccgt ccgccagcgc cgggagcgcg ggggcgggcg
     2701 cggggggggc attgtcaggc gtctggcccg cctggcccgg cgcggcggcg gccggggctc
     2761 ccgccgcgcc gccggtgtcg gcgcccgccg cctgcccttc ccgcgcggcc ggctgctggg
     2821 cggtggcggc ggggctgttc gtcccgccgc cgggcttcgt gcagcgcccg tccttcacgc
     2881 aggccagcag caggcccgcc atgaggatgc gcaacgcggg cggccaagac gcccatgcgc
     2941 ccgcgatcgc ggatgcgagg cgcttgaaga tccggcgctc gtggcaaaca gcggcgatcc
     3001 atgcggaata gagcgccagc acgaggagtg tctgccagcg catctcgggc gtgctcataa
     3061 ccggcctcca agcacaaaac tatcagtcaa atcccgcatc cgagcctttt cccgttccat
     3121 tctcttcccc ctttgcctcc cgcctctaag tgccccgcgc aaaaaggcgc gaaccccaat
     3181 ctgtgcctca tctgaggtac caccaagcac ctgttaggaa acacgagaca gaggccgcgc
     3241 ccgagcgggc gcgtccccgt gcatctcgct tcctaactta aacttggtgg tttcagatga
     3301 agcgttaatg cgcgtaagcg caaaaatcaa atcagtctgt ggggtccggt cttttccgca
     3361 tgcgttgtcc ttgtcggttc aattactcca gccaatcatc gggataaaca tactcctttt
     3421 tccggaaagt tcgcaaccgt ttttttcagg ttgcgggttg aaggggacag gttacacgtt
     3481 gcgggttgcg gattgcaggt ttcggcgact cctcctgcct acctgccaca acttgaacgt
     3541 tgggtgtcga acgttgagcg ttcttcaccc ctctgccacc aaccggttac cctctgcgct
     3601 tgtctcaccg ttcaacattc tgtatgctct ctttacaaat gaaacctgag acttcttttc
     3661 cttttctaaa aagcaaagcc tcttggttgg ctgcgccgct gttctggatc acaggtgttg
     3721 tcgcgctt
//
LOCUS       DAOSRB010000257         3726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_22215_length_3726_cov_7.285481, whole genome shotgun sequence.
ACCESSION   DAOSRB010000257 DAOSRB010000000
VERSION     DAOSRB010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22215_length_3726_cov_7.285481"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1556)
                     /locus_tag="PLZ74_10425"
     CDS             complement(201..1556)
                     /locus_tag="PLZ74_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOR98774.1"
                     /translation="MTDQDLFDKSASSPEFPTGHAAEGLRPPLAARMRPETLEDVVGQ
                     EHILGPGKLLRRVIEADRLTNLIFYGPPGCGKTTLAEVISRITRRRFERTSGVLSNVA
                     TLRSLCETARLYRNDPGTILFIDEIHRFSKSQQDVLLPYVENGTVTLIGATTHNPNIF
                     VNSPLTSRSLVFELRPLSEEAITCLVQRALADSSRGLGHLPAELTPEATQFLATMCDG
                     DARRALNALEIAVRSTPPDPDGVIRITHAVIEDSVQRKMVVYDKDEDGHYDTISAFIK
                     SVRGSDPHAAIYWLAKMLEAGEDPRFIARRLIILASEDIGNADPRGLTVAVAAMHAVE
                     FIGMPEARISLAQATTYLATAPKSNASYLAVEEALADVRSGRVLAVPEHLKNVHVRAV
                     GREGLDTAYKYPHDYAQHTVDQLYLPKDKRYYRPAAQGYEETLARRMEHVERLKRETR
                     E"
     gene            complement(1600..2451)
                     /locus_tag="PLZ74_10430"
     CDS             complement(1600..2451)
                     /locus_tag="PLZ74_10430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOR98775.1"
                     /translation="MFVPNADNITARWVCQIAGGGILVAGVAAALALLWSAQRRRAAG
                     ARTVAVAHLASLPLSDVHAGVTLIITFLFALASLPRTAGTAFRPDDRALVLGPLLYLC
                     SSVLVVMFCGVHARCGLRRMFLAGPVRPAAAVAKGLVYGLAAIPPVALLSFLISAGLE
                     AWGFAPQHQEVFDWLGDASRAPGARLFMMGAAVFLAPLSEETLFRGILFPCLLRGRSV
                     VGAALVSSLYFATVHLHLASFLPLVVLGMFFSAGYAATGSLLTPITMHALFNFTSLIF
                     YLADPLP"
     gene            complement(2456..3151)
                     /locus_tag="PLZ74_10435"
     CDS             complement(2456..3151)
                     /locus_tag="PLZ74_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOR98776.1"
                     /translation="MNIAGVVPLSLCDYPGRVAAVVFTQGCNFRCPFCHNAHLIPLLP
                     VAGQMLEEEQVFARITEYRTRLDGVVVSGGEPTLQQDLPEFLLRLKTLGLAVKLDTNG
                     SQPEMLRRVLDERLVDFIAMDVKAPWEHYPRLTGTACAQDDLLRSLRLIAGSGLPHQF
                     RTTRVDPLLTDSDYRNVRAQIPDGSPHVWQAFRPEHAFDPALRPPRPCTDNVPVSVSS
                     RLSDCPQARERAQ"
     gene            complement(3148..>3726)
                     /gene="nrdD"
                     /locus_tag="PLZ74_10440"
     CDS             complement(3148..>3726)
                     /gene="nrdD"
                     /locus_tag="PLZ74_10440"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOR98777.1"
                     /translation="AFALTVMDFMRARLADYQEETGNLYNLEATPAEGTTYRLARLDR
                     KLYPEIITAGGEGEPFYSNSTQLPVNYSDDILRVLDLQDEIQTKYTGGTVVHAFIGEA
                     VSDPQAVKAFVRMVCQNYRLPYFTLTPTFSICEDHGYLSGEVHECPHCGQYTEVYSRV
                     VGYIRPIQQWNAGKQNEFHQRKTYHVCEAAAR"
BASE COUNT          719 a         1115 c         1245 g          647 t
ORIGIN      
        1 tatcggtatc gggatcgcta tcgggatcgc tatcgggatc gattttcctg atacgtggtc
       61 ctgatagaaa gaaatgaccg gtacgacaat caccccggat atctgcctgt cagccggtct
      121 gtgcagatca atgcctgtgc aaagcgcgtg gccgctttca aaacttcagg tgttgcgctg
      181 gccggtcgcg cgggcatgcg tcattcgcgt gtttcgcgtt tcagccgttc gacgtgttcc
      241 atgcgccggg cgagggtttc ctcatatccc tgcgcggcag gacggtaata gcgcttgtcc
      301 ttcggcagat acagttgatc cacggtatgc tgggcataat cgtgcggata cttgtaagcc
      361 gtgtcgagtc cctcccgccc cacggcacgg acatgcacgt tcttgaggtg ctccggcacc
      421 gccaagacac ggccggagcg cacgtccgcc agagcctctt ccacggcgag gtacgacgca
      481 ttgctcttgg gcgcggtcgc cagataggtg gtggcctgcg cgagggagat acgggcctcc
      541 ggcataccga tgaactctac ggcatgcatg gccgcgaccg ccaccgtgag tccgcgcggg
      601 tcggcattgc cgatgtcttc ggaggcgagg atgatcagac ggcgcgcgat gaaccgcggg
      661 tcttcgccgg cctccagcat tttggcgagc cagtagatcg cggcgtgggg atcggaaccg
      721 cgcaccgatt tgatgaaggc cgaaatggtg tcgtagtggc cgtcctcatc tttatcatag
      781 acgaccatct tgcgctgtac cgagtcctcg atcacggcgt gggtgatgcg gatcaccccg
      841 tccggatcgg gcggcgtgga gcggaccgcg atctcaagtg cattcagcgc gcgccgcgcg
      901 tctccatcgc acatggtggc gagaaactgt gtggcctcgg gcgtgagttc ggccggaaga
      961 tgacccaatc cgcgcgagga gtcggccagc gcgcgctgca ccagacaggt gatggcttcc
     1021 tcgctcagcg gtcgcaattc gaacaccagg gagcgtgagg tgagcggact gttgacgaag
     1081 atattcgggt tgtgggtggt ggcaccgatg agggttaccg tgccgttctc aacatacggc
     1141 agcagcacat cctgctgcga tttgctgaac cggtggatct cgtcgatgaa caggatggtg
     1201 ccggggtcgt tccggtagag gcgggcggtc tcacagagag agcgtaacgt ggcgacatta
     1261 gacagaaccc cgctggtccg ctcaaaccgt ctgcgggtga tgcgggagat gacttcggcc
     1321 agggtggttt tgccgcatcc gggcggcccg tagaaaatca ggttggtcag gcggtccgcc
     1381 tcaatcacgc ggcgcaacag ttttcccggg ccgagaatat gctcctgccc caccacgtct
     1441 tcaagcgttt cgggccgcat gcgtgcggcc aacggggggc ggaggccctc tgcggcgtgt
     1501 cccgtcggga actccggcga tgacgccgat ttgtcgaaaa gatcttgatc cgtcatgtgc
     1561 gtgttctgct tgtgggggtg gccggcttgc gcgccggatt cagggaaggg gatcggcaag
     1621 ataaaagatc aggctggtga aattgaagag cgcgtgcatg gtgatggggg tcaggagcga
     1681 gccggtggca gcgtaacccg cagaaaaaaa catgcccagc accaccagcg gcagaaagga
     1741 cgcgaggtga agatgcaccg tcgcgaaata gagggaggaa accaatgcgg cgcccacaac
     1801 cgacctcccg cgcagcaggc atgggaagag gatgccccgg aacagggtct cctcggaaag
     1861 gggggcgaga aacacggccg cccccatcat gaagaggcgt gccccgggcg cccgcgaggc
     1921 atcgccaagc cagtcaaaga cctcctgatg ttgcggtgcg aaaccccaag cctccagacc
     1981 tgcggaaatc aggaacgaga gcagggccac cggcgggatc gcggcgagtc cgtagaccag
     2041 ccctttcgcc actgcggcgg ccggcctaac cggtccggcg agaaacatcc tccgcaggcc
     2101 gcacctcgcg tggacgccac agaacatcac gaccagcaca ctcgaacaga gataaagcag
     2161 gggaccgagc accagcgcgc ggtcgtcagg ccgaaacgcg gtccctgccg tccgaggcaa
     2221 ggacgccaga gcgaacagga acgtgatgat cagggtcact ccggcgtgaa catccgagag
     2281 ggggagcgag gccaggtgcg cgacggcgac ggtccgcgcg cctgcggcgc gacgacgttg
     2341 cgcggaccac aacagcgcga gcgctgcggc aactcccgcg accagaatcc ccccgcccgc
     2401 gatctggcag acccagcgtg ccgtgatgtt atcggcgttc ggaacgaaca tgcctctatt
     2461 gtgccctctc ccttgcctgc gggcaatcag aaagacggga cgacacgctt acgggcacgt
     2521 tatcggtgca cggacgggga gggcgcagcg cgggatcaaa cgcgtgttcg ggccggaagg
     2581 cttgccagac atgcgggctg ccgtccggaa tttgcgcgcg cacattacgg taatcgctgt
     2641 ccgtcagcaa cgggtccacg cgcgtggtgc ggaactggtg gggaagcccg gaaccggcga
     2701 tcagccgcag gcttcgcaag aggtcatcct gagcacacgc ggtgcccgtg agtcgcgggt
     2761 agtgttccca cggggccttg acatccatgg cgatgaaatc cacgagacgc tcgtcgagca
     2821 cgcggcggag catttccggc tgcgaaccgt tggtgtccag tttgaccgcg aggccgagcg
     2881 ttttgaggcg cagcaaaaac tccggcagat cctgctggag ggtcggctcg cctccggaga
     2941 ccacgactcc gtccagacgg gttcggtact ccgtgatccg ggcgaacacc tgttcttcct
     3001 ccagcatctg tccggctacg ggcaggaggg ggatcaggtg cgcattatgg cagaaggggc
     3061 aacggaaatt acagccctgc gtgaacacca cggcggccac acggcccgga taatcgcaga
     3121 ggctgagcgg cacaaccccc gcgatgttca tcgcgccgcc gcctcgcaga cgtggtaggt
     3181 tttgcgctgg tggaactcgt tctgcttgcc cgcgttccac tgctgaatag gccggatgta
     3241 ccccaccaca cgcgaataga cttccgtgta ctgtccgcag tgcgggcact catgcacctc
     3301 gccggacagg tagccgtgat cctcgcagat gctgaaggtc ggcgtcaacg tgaagtacgg
     3361 caaccggtag ttctggcaga ccatgcgcac gaacgccttg actgcctgcg ggtcgctgac
     3421 cgcctcgccg atgaaggcgt gcacgaccgt gccgccggtg tacttggtct gaatctcatc
     3481 ctgcagatcc agcacacgca ggatgtcgtc gctgtaattg accggaagct gggtcgagtt
     3541 ggagtagaag ggctctccct cgccgccggc cgtgatgatc tcggggtaga gtttccggtc
     3601 gagccgggcg agacggtagg tcgtgccctc ggccggggtc gcctccagat tgtagaggtt
     3661 gccggtctct tcctgatagt cggccagccg cgcgcgcatg aaatccatca cggtcaaggc
     3721 aaaggc
//
LOCUS       DAOSRB010000258         3702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_22411_length_3702_cov_7.167261, whole genome shotgun sequence.
ACCESSION   DAOSRB010000258 DAOSRB010000000
VERSION     DAOSRB010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22411_length_3702_cov_7.167261"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..1509
                     /locus_tag="PLZ74_10445"
     CDS             529..1509
                     /locus_tag="PLZ74_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOR98778.1"
                     /translation="MDNATILSRAREVMETEIEGLRAAQAALGDDFLATVRLLTETLD
                     RDGKIVVTGVGKNLHIAEKLSATLASTGSTSVVMNPIQAMHGDLGMIAARDVLLALSF
                     SGESEEVIRLIPAVRRLEVPVIAMVGNPESTLARLSDVTLCVRIGREACPFGMAPTTS
                     TTATLAVGDALAMVLIDARQFDKASYARLHPAGAIGRALVLRVRDIMRTGERLASLRS
                     DASIMDAVMAMTAAQSGAALMTDPEGRLVGIFTDGDLRRLLSEGRAGLHEPLAPVMTR
                     NPKSVADDAFAVDVLRVFERYKIDDLPVVDARGLLVGVVDIQDLPKMKVM"
     gene            1744..2478
                     /locus_tag="PLZ74_10450"
     CDS             1744..2478
                     /locus_tag="PLZ74_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOR98779.1"
                     /translation="MMFGWGVQIMAQITEPQGRLAAAFTLFDRGGPVMWPILAVSLMG
                     LTVTFERLFAFWRYNTANFCFRRRQSKLIALTREGRFEEAVACARQTDSPVCRVLVKA
                     LENRDSGFQETLEAASQLELDRLRRGLSVLDTTITVAPMLGILGTVTGIINTFNMLNL
                     EGIENPAAATAGIAEALITTAAGLIVAIACLFPFNFLVSQLKRRTHELEQIIHQFALA
                     FNTGVRVAEKPGTGAGTGRCRNVTEE"
     gene            2482..2883
                     /locus_tag="PLZ74_10455"
     CDS             2482..2883
                     /locus_tag="PLZ74_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOR98780.1"
                     /translation="MRLNSGYEDRKARLEMISLMDVMFLILVFFVYSIFSMAVHRGLK
                     VELPAARGVPEQGERVIVTLAADGTLSLNRRPLALEDLVRGVTLLWRETGTPVLISAD
                     RAAPLGVGIELLGRLKEGGVERVTFQVSGKP"
     gene            2900..3691
                     /locus_tag="PLZ74_10460"
     CDS             2900..3691
                     /locus_tag="PLZ74_10460"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOR98781.1"
                     /translation="MRAVILHLLCFALAALLHLLLLLLVGWHLVERGKEVWPELEVAS
                     VELTLEGPAPETPAVMSRRTVPEATPPPEPAALPEPPQDLPPLPEATPLSPPESLPTP
                     PEPRPLPEPARAPVRELPEPEARPVPEPVRVPVREQPEPEARPVQAEPGPQVEEAEAE
                     IREQGGAAVGQIIGHPLLEQTIRPTYPVGARRRGEEGTVILDVTVGPDGRAVTVTQVA
                     SSGFPDLDRAAERAAAQARFKPGTRDGKPVTSAARLTLIFRLRDR"
BASE COUNT          662 a         1106 c         1203 g          731 t
ORIGIN      
        1 agtcatcaag cgacagcctg cgttctccct gtccccacgc cgtccctgcc agccataccc
       61 cacagacgat ccattgcaaa acgtttcgcg tcacaacgac tctccttcgg tagttcagaa
      121 gttatccttt cccatgtaca gtaatattat gccgtatttt cgtatcctga aacacataaa
      181 actgtaatcg gttcgtgaca ggggggaaga gtcatcgtgt acgggtacgt gacgagtacg
      241 tgtacgggga ggggccaaca ttcacgttct ccaaatcgct atcggtatcg ctatcgggtt
      301 cgggttcggg aacgtcgacc cgcgacccgc gacattcgac ccgtgacgat cgacagggac
      361 gcccgcgttt ccgacaccgc aggtcgctcg tcgcgggtcg cgagtcaacc gccccgatcc
      421 cgatggcgat agcgatggcg ccgcaatgga accctgaaac gcatgacctt ggaaatcctt
      481 gattcctccc ttgcttcccg aaaccgttgc ggtacaataa aacgcattat ggataacgca
      541 acgattctct cccgcgcccg ggaggtgatg gaaaccgaga tcgaggggct gcgcgcggcg
      601 caggccgctc tgggggatga ttttctggcg accgttcgtc tgctgacgga aacgctcgac
      661 cgcgacggta agattgtggt cacgggtgtg ggtaaaaatc tccacatcgc cgagaaactc
      721 tcggccacgc tggccagcac cggttcgacc agtgtggtga tgaaccccat tcaggccatg
      781 cacggtgacc tcgggatgat cgcggcccgg gatgtgctgc tggcgctgag tttttcgggc
      841 gagtcggaag aggtcatccg gctgatcccc gctgtgcggc gtctcgaggt gccggtcatc
      901 gccatggtcg gcaatccgga gagcaccctc gcgcggttaa gcgatgtgac gctgtgtgtg
      961 cgtatcggac gggaggcctg cccctttggt atggcgccca ccaccagcac taccgcaacc
     1021 ctcgccgtag gcgacgcgct ggctatggtg ctgatcgatg cacgccagtt cgacaaagcc
     1081 agttacgccc ggctccatcc ggcgggtgcg atcggccgcg cactggtcct gcgcgtgcgc
     1141 gacatcatgc gtacgggtga acgtctcgcc tccttgcggt ccgatgcctc gatcatggat
     1201 gcggtgatgg ccatgactgc ggcccagagc ggcgcggccc tgatgaccga tccggaagga
     1261 cggcttgtcg ggatctttac ggacggcgat ttgcgtcggc tgctttccga aggccgggcg
     1321 ggtctgcatg agcctctggc accggtgatg acccgcaacc ccaaatcggt cgccgacgac
     1381 gcctttgcgg tggatgtgct gcgcgtgttc gaacgctaca agatcgacga tctgccggtg
     1441 gtggatgcgc gcgggctcct cgtcggggtt gtcgatattc aggatctgcc caaaatgaag
     1501 gtgatgtgaa ccgtgggcaa gggtgaagcg tttgaagaga cgaagcgaga attcacagcc
     1561 gggattacag gactcacatg attaccgaga atcaaaaaac cctgtaagtc ctgtaatccc
     1621 ggctaaagaa ttgggctgcg ggttgtcccg tatggtttga acgtgtggct gctttgggta
     1681 gggacgcttc cccaaggcgt caggacggtt cggggaaccg tccctaacgg taaggaacgt
     1741 tgcatgatgt ttggatgggg cgtacagatt atggcgcaga ttacggagcc gcaggggcgt
     1801 ttggcagcgg ccttcacgct gtttgaccgc ggcggtccgg tgatgtggcc aatcctggcg
     1861 gtttcgctga tggggctcac ggtgacgttc gagcgtctct tcgccttctg gcgctacaac
     1921 accgcgaatt tttgtttccg cagacgtcag agcaagttga tcgccctgac ccgcgagggt
     1981 cggttcgaag aggcggtcgc ctgtgcgcgt cagaccgatt cgccggtgtg ccgcgtactt
     2041 gtcaaggcat tggaaaatcg cgattcgggg tttcaggaga cactcgaagc cgcctcgcag
     2101 ttggagttgg accgcctgcg ccgggggctc tcggtgttgg acaccaccat cacggtcgcg
     2161 ccgatgctcg gcattctcgg tacggtcacg ggtatcatca acacgttcaa catgctcaac
     2221 cttgagggta tcgagaatcc ggcggccgct accgcaggca ttgccgaggc cctgatcacc
     2281 acggcggcgg gactgatcgt ggccatcgcc tgcctgttcc cgttcaattt tctggtgtcc
     2341 caactcaaac gccgcaccca cgagttggag cagatcatcc atcagttcgc gctggccttt
     2401 aacaccggtg tccgcgttgc ggaaaaaccg gggacggggg ccgggacagg acgatgccgc
     2461 aatgtgacag aagagtgacc tatgcgcctg aattcaggat atgaggaccg caaggcccgg
     2521 ctggaaatga tctccctcat ggatgtcatg ttcctgatcc tcgtcttctt cgtctattcg
     2581 atcttctcca tggcggtcca ccgcggcctg aaagtggagt tgcccgcggc gcgcggggtg
     2641 ccggagcagg gcgagcgggt cattgtcact ctggcggcgg acggcacgtt gtcattgaac
     2701 cgacggccgt tggctttgga ggacctcgtg cgcggcgtga ccttgctctg gcgcgagacc
     2761 ggcacgccgg tgctgatcag cgcggatcgg gcggcgcccc ttggtgtcgg gatcgaactc
     2821 ttgggccggc tcaaagaggg cggggtcgag cgcgtcacct ttcaggtgag cgggaaaccg
     2881 tgaggggagg cctcatccca tgcgggcggt gatccttcat cttctctgtt tcgcgcttgc
     2941 cgcgctgttg catctgctgc tgctgctgtt ggtcgggtgg catttggtcg agcgcgggaa
     3001 ggaggtgtgg cccgagttag aagttgcctc ggtggaactg acgttggagg gtcctgcgcc
     3061 ggaaacaccc gcagtcatgt cacggcgtac ggtgccggag gctacgccgc ctcccgagcc
     3121 tgccgcatta ccggaacctc cgcaggattt accgccgctt ccggaggcga cgcccttgtc
     3181 tccgccggaa tctctgccga ccccgccgga accgcgtccc ctgccggaac ctgcccgcgc
     3241 cccggttcgg gaactgcccg agccggaagc gcggcccgtg ccggagcctg tccgcgtccc
     3301 ggttcgggaa cagcccgagc cggaagcgcg gcccgtgcaa gcggaaccgg gtccgcaggt
     3361 ggaggaggcg gaggctgaaa tccgggaaca aggcggagcc gcggtcggcc agatcatcgg
     3421 gcatccctta ctcgaacaga cgatccggcc gacctatccg gttggcgcgc gccgacgcgg
     3481 cgaggagggc acggtgattc tcgatgtgac ggtggggccg gacggccgtg cggtaacggt
     3541 gacacaggtg gcttccagcg gtttccccga tctggaccgc gcggcggaac gtgccgcggc
     3601 tcaggcacgc ttcaaacccg gcacacgcga cggtaaaccg gtcacttccg cagcccgtct
     3661 cacgctgatc ttccgcctgc gcgaccggta aacctaaaaa ga
//
LOCUS       DAOSRB010000259         3660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_22763_length_3660_cov_7.073509, whole genome shotgun sequence.
ACCESSION   DAOSRB010000259 DAOSRB010000000
VERSION     DAOSRB010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22763_length_3660_cov_7.073509"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1763)
                     /gene="lnt"
                     /locus_tag="PLZ74_10465"
     CDS             complement(63..1763)
                     /gene="lnt"
                     /locus_tag="PLZ74_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOR98782.1"
                     /translation="MITRLRKGWVKGAAAVASGLLMWAAFPPQAQVHGMWMALAPLFL
                     VIRRSRPREAFGWSWLCGMLFWLLTLSWFPAIIRNNGPWFLVLLGQGALAAWCALFLA
                     MTGAASARVWGWAGQTPGWRRVATVAVFDPLLWVGAEVVRGWLFSGFAWNFLGVSQVD
                     NTALIQVASITGVYGVSALLVMGNGAVASLLERMAEPFVARCRPVPRTTVPAYGWAGR
                     FARSIESFLPLVVVLAVWFWGMGRIRVWDREARRSGVYRIALIQPNAPCIFEVSDETV
                     RMQWEQLVSQSRLVGAAKPDLVVWPETAVMGSVPYDTDTMRVIREGATAANAPLLTGA
                     LEVERAGVSRVTPDGCRYYNAAWLVSATGETLGCYRKRHLVPFGEYIPGDKLVPWLQR
                     FAPTGVSCTPGRGPGPLHVARGAGRILSLGPLICFEDTIPALSRGAVRAGARLLVLMT
                     NDAWFSGSVEPLQHQQQAVFRAVENGVPLVRAANSGVSCVVDAVGRVKRLESGEAITD
                     FDGFLVMPVTVPEVPLRAPYTAWGDWVLGIPGAVLLATLLVVGAVRDRRARRREEDAE
                     "
     gene            complement(1773..2297)
                     /locus_tag="PLZ74_10470"
     CDS             complement(1773..2297)
                     /locus_tag="PLZ74_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOR98783.1"
                     /translation="MSEPLLGLVTGCLFGFLMQKAGVLRFDRQVGALMFRDMTIIKFM
                     LSAILVGSVGLQLLAEAGQITMSPRTLNTGVIVGGLLFGAGWAVAGFCPGTAVGAVAE
                     GRWHALFVIAGMFAGAALYAEAEPWLKTSVQAWRDFGPLTLSQVTHLSPWYCIALLWG
                     VGTVTFVWCAYKKV"
     gene            complement(2290..2838)
                     /locus_tag="PLZ74_10475"
     CDS             complement(2290..2838)
                     /locus_tag="PLZ74_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOR98784.1"
                     /translation="MISKKRERRWNPYLTGALTGLLAIASAYATTRVTGRTVYLGAST
                     TIVRATAEIERRILPGHASRNAYLKAHPGVWDWQAGLVCGIAIGALIAALSGRTFESE
                     WVPERWRKRFGPNVAKRAAGGFLGGAAALFGARLAGGCPSGLGLSGAMQLSVSGLVGM
                     ICFFTGGVFLIRLIGKRRDAYE"
     gene            2976..3335
                     /locus_tag="PLZ74_10480"
     CDS             2976..3335
                     /locus_tag="PLZ74_10480"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOR98785.1"
                     /translation="MYCTLLKAKLHHLRVTQANKEYEGSLTIDEDLMDAVGILNFEKL
                     LIANLDNGERFETYAIRGPRGSGVACLNGAASYKGQIGDRLIVMVWCLLDDTERAAHH
                     PKIVRLDDRNRIVATAL"
     gene            3528..3603
                     /locus_tag="PLZ74_10485"
     tRNA            3528..3603
                     /locus_tag="PLZ74_10485"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3561..3563,aa:Ala,seq:ggc)
BASE COUNT          756 a         1364 c          975 g          565 t
ORIGIN      
        1 aacgaaaacc tccatgtcgg acagcgtgac ggccgacgaa caccgctcca actcctcccg
       61 ggtcattccg catcctcctc tctccggcgc gcgcgccggt cccgcacggc accgaccacg
      121 agcagggtcg cgagcaagac cgcgccgggt atccccaaca cccaatctcc ccatgccgtg
      181 tacggcgcgc gtaacggaac ttccggcacc gtcacgggca taaccagaaa tccgtcgaaa
      241 tccgtgatcg cttcgccgga ctccaatcgt ttgacgcgtc ccaccgcatc caccacgcac
      301 gacacaccgg agttcgcggc ccgcaccaac ggcaccccgt tctccacggc acggaacacc
      361 gcctgctgct gatgctgcaa cggctcgacc gagccgctga accacgcgtc gttggtcatc
      421 agcaccaaca gccgcgcgcc ggcccgcacc gccccgcgac tcaatgcggg gatcgtgtcc
      481 tcaaaacaga tcagcggtcc cagactcagg atccggcccg cgccccgtgc cacatgcagc
      541 ggccccggcc cgcggcccgg tgtgcaactc acgccggttg gcgcgaaacg ctgtagccac
      601 gggacgagct tgtcgccggg gatatactcg ccgaacggca ccagatgccg cttgcggtaa
      661 caccccaatg tctcgccggt cgcggaaaca agccaggcgg cgttatagta gcgacacccg
      721 tcaggcgtca cgcgagaaac cccggcacgc tcgacctcca acgcgccggt cagaagcggg
      781 gcattggctg ctgtcgcacc ctcgcggatc acacgcatcg tatcggtgtc gtagggtacg
      841 gaacccatga ctgcggtctc gggccagacc accagatccg gcttggccgc gcccacgaga
      901 cgggactggc tgaccagctg ttcccactgc atccgaaccg tctcgtcgct gacctcaaaa
      961 atgcagggcg cgttcggctg gatcagcgcg atccggtaca cgccgcttcg ccgtgcttcc
     1021 cggtcccaca cgcggattcg tcccataccc caaaaccata cggcgagcac cacgaccagc
     1081 ggcagaaaac tttcgatcga acgcgcaaac ctccccgccc aaccgtacgc cggaacggtc
     1141 gtccgcggaa ccggacggca ccgcgcgacg aacggttccg ccatccgctc caacaggctc
     1201 gccaccgcac cgttgcccat cacaagcagt gcggacacac cataaacacc cgtgatcgac
     1261 gccacctgga tcaacgccgt gttgtccacc tggctgaccc ccagaaagtt ccaggcgaat
     1321 ccggagaaca accaccccct cacaacctcg gccccgaccc acaacaaggg gtcgaacacc
     1381 gccaccgttg ccacgcgccg ccatcccgga gtctgccccg cccatcccca gacgcgggcc
     1441 gaggccgccc cggtcatggc cagaaacagt gcgcaccacg ccgccagcgc tccctgtccc
     1501 agcagcacca gaaaccatgg gccgttgttg cggatgatcg ccggaaacca gcttagtgtc
     1561 agaagccaga agagcatccc gcatagccaa gaccagccga acgcctcacg gggacggctc
     1621 cgccggatca ccagaaacag cggggccagc gccatccaca tgccgtgaac ctgcgcctgc
     1681 ggcggaaacg ccgcccacat cagcagtccg cttgcaaccg ccgccgcccc tttgacccac
     1741 cccttcctca gtcgcgttat cacccttaat ccctacacct tcttatacgc gcaccagaca
     1801 aacgtcaccg tccccactcc ccacagcagc gcgatgcaat accacggcga aaggtgcgtc
     1861 acctgcgaca gggtcagcgg tccgaaatca cgccacgcct gcacgcttgt tttcagccaa
     1921 ggctcggcct cggcatagag cgccgcaccc gcgaacatgc ccgcgatcac gaacagcgcg
     1981 tgccaccgac cctctgcgac cgccccaacc gccgtacccg gacagaatcc cgcgaccgcc
     2041 catcccgcgc cgaacagcag ccctccgaca atcacacccg tattcagcgt ccgggggctc
     2101 atcgtgatct gccccgcctc cgccaacagt tgcagcccca cgctgccgac cagaatcgcc
     2161 gacagcatga atttgatgat cgtcatatcg cggaacatca gggcgccgac ctgtcggtcg
     2221 aagcgcagca ccccggcctt ctgcatcagg aacccgaaca aacacccggt caccagaccc
     2281 aaaagcggct cactcatacg cgtccctccg cttgccgatc aaccggatga ggaacacccc
     2341 gcccgtgaaa aaacagatca tcccgaccag gccactcacc gaaagttgca tcgccccgct
     2401 gagcccgagc ccgctcggac acccccctgc caaccgtgcg ccgaacaagg ccgccgctcc
     2461 gcctagaaac ccgcctgccg cgcgtttcgc cacattcggc ccgaaacgct tgcgccaacg
     2521 ctccggaacc cattcggatt cgaaggtgcg gccgctcaac gccgcgatca gggcacctat
     2581 ggcgatcccg cacaccaaac cggcctgcca gtcccagacc ccggggtgcg ctttcagata
     2641 cgcattccgg gacgcgtgcc ccgggaggat ccgccgctcg atttccgctg tggcccgcac
     2701 aatcgtggtg gaggctccga gatatacggt gcgtcccgtg acccgcgttg tggcgtaagc
     2761 cgacgcgatt gcgagcaggc cggtcaacgc gcccgtcaga tagggattcc agcgtcgctc
     2821 cctttttttg ctgatcattc cgtctccttt tccgcgtccg gactggacgg ggattaacga
     2881 taacacgccc cgaaagacgg gtcaatcgcg cacgaatccg ctcttgcata atccggcata
     2941 cccgcttatc ctgtgccgcg ctttaaagga atcgcatgta ctgcacattg ctgaaagcca
     3001 aactgcacca cctccgggtg acgcaggcga ataaggagta cgagggcagc ctgacgatcg
     3061 acgaggatct gatggacgcc gtcggtattc tgaatttcga gaagctgctg atcgccaatc
     3121 tcgataacgg cgagcggttc gaaacgtatg ccatccgcgg tccgcgcggc agcggcgtgg
     3181 cgtgcctcaa tggtgcggcc tcgtataaag gccagatcgg cgaccgcctg atcgtgatgg
     3241 tgtggtgcct gctggacgac accgagcgcg cggcgcacca cccgaaaatc gttcgccttg
     3301 atgaccgcaa ccgcatcgtg gccaccgccc tctgaacgcg caccctgccg aacgcgacgc
     3361 gattgtgcgg gcagggattc ccgcgcatcc ggcttgtccg cccttttttc acctgagccg
     3421 tgcccatcct taccgccaac cgattccaaa tcttttcttc tgcgcgattg acatccgcag
     3481 tcggggatga ttaaatgacc cgacttttcc gcatgggaaa ggcccgtggg ggtatagctc
     3541 agttggtaga gcgcttgaat ggcattcaag aggtcggggg ttcgactccc cctacctcca
     3601 ccatatataa aaaaataaac ccgggtgctt atgccgggca gtcctttcca gaacgtcacg
//
LOCUS       DAOSRB010000260         3611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_23158_length_3611_cov_5.804556, whole genome shotgun sequence.
ACCESSION   DAOSRB010000260 DAOSRB010000000
VERSION     DAOSRB010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23158_length_3611_cov_5.804556"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PLZ74_10490"
     CDS             complement(<1..1164)
                     /locus_tag="PLZ74_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOR98786.1"
                     /translation="MIKKLTLQVRLGTSFRVACAVLGIVLVSSGAPLTEREALDRLSI
                     WINGHPVMGCLAGNAIASTVRFQEGGGSYSVYAVAFSPVGYVVLNSDDRLPLVVAFSA
                     SSGVNLADLPDNAFRAALLTHVANAAETLGGMDTALAPDLSYRRTAPRRAPLSIEQYG
                     PYLATIWNQCNPYNLLCPEDPGGSAYYGYRVPSGCTPAAYAQVLHYHRWPLRGQGSHS
                     YTDSNGSITGTHSVCFSTPFGWAAMQAAYDPWTTTPQPGDVEVADLMYRLGVAADADY
                     ESDGTASSIRTLGERLNTYLFYEPIGYASSQTALLPGLNESLRTGYPCVVAVPGHAIA
                     ADGLLDDNGTVSYHINYGWGGSNNGWWSADNVAGQALAYGCTSLKPMLMAFPAE"
     gene            complement(1157..2431)
                     /locus_tag="PLZ74_10495"
     CDS             complement(1157..2431)
                     /locus_tag="PLZ74_10495"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOR98787.1"
                     /translation="MSGSFPKLPERTPAQEDLLRVMRAAVNGQVAEIDPLAWPAVYRL
                     AAMHQVETFLYPLIRAWPPAALPPEEMMHFWRTVFLQAAGRYARVAEQARELFSALHA
                     AGIEMIPLKGVWLAERIYMEGGCRTMNDIDLLVPEARFNEACGVIRSIGYVTPDYDVT
                     DRRVKHAHFQKCGEPLALELHRRLFSHPVSAGEAPSTDWVWHGNMEDRLHGIPVQTFA
                     PERQVAYLAHHILGHRMAVPLKVYLDLCLVCERFAAPLSDTAFRHERARWGAVYGISF
                     VLRMADRLLKSEACRMWAAIPEESAEMCEALDVAEQIVVGLDAKSIKLTPSLSRFIRS
                     KGLERIRIGLASLFRSAAEMRQIYPRTVRWFGLAGGYLWRLPDLFLRHGSFLSGSPSC
                     RRQTKVAHKGFEDREQLIAWLCARENDTKGHD"
     gene            complement(2412..>3611)
                     /locus_tag="PLZ74_10500"
     CDS             complement(2412..>3611)
                     /locus_tag="PLZ74_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR98788.1"
                     /translation="WRREQAPLEREAGYVHHLLTDGAYAKEIRVMGHGTFCRNRFTAV
                     RSRLRAARLRLLRLVLTQELAMQFLALATMGVLLLWMAGRLAAGALSLGALVMYAQAV
                     QRGQAQTGALAGTLAELYQSALFLESFDTLLAKPDTVRTPSRPRALPAPGCHGLVFER
                     VSFTYPGTSRPVLREISFTLHPGERIALVGANASGKSTLLKLMCRFYDPTAGRILFEG
                     VDLREVDPVVWRQRIGALFQDFGQYQFSLAENVWIGDPHGSASDARIAETVRRVGLDA
                     LTRTDPRGLDTPLGRWLREGIEPSLGQWQRVAVARATLRDADLLLLDEPASALDDRAQ
                     DEVIQWLQTVTVGRMAVLASHRRRVIEVADRVLVLREGVLVEEGTIPALRARKGEWAS
                     LYERVLS"
BASE COUNT          715 a         1183 c         1091 g          622 t
ORIGIN      
        1 ctcggccggg aatgccatca gcatgggctt gagcgaggtg cagccgtatg cgagcgcctg
       61 gccggccaca ttgtcggccg accaccaacc gttgtttgag ccgccccagc cgtagttgat
      121 atggtacgac acggttccat tgtcatccag caacccatcc gccgcgatcg cgtggccggg
      181 caccgcgacg acgcacggat agcccgtgcg caggctctcg ttaagtcccg gcagcagtgc
      241 ggtctgcgac gacgcatagc cgataggctc gtagaagagg tatgtgttga ggcgctcgcc
      301 cagcgtccgg atacttgagg cggtgccgtc actctcgtaa tcggcatcgg cggcaacacc
      361 caggcggtac atgaggtcgg cgacttcaac gtcgcccggc tggggtgtgg tggtccaggg
      421 gtcgtaggcg gcctgcatgg ccgcccagcc gaatggcgtg ctgaaacaga cgctgtgcgt
      481 gccggtgatc gagccgttgc tgtcggtgta gctgtgcgag ccctgcccgc ggagcggcca
      541 gcggtggtaa tggagcacct gagcgtaggc ggcaggcgtg cagccggacg gcacgcggta
      601 tccgtagtat gcgctgccgc cgggatcttc agggcacaac aggttgtacg ggttgcactg
      661 gttccagatg gtcgccagat aagggccgta ctgctcaatg ctcaatggcg cgcggcgggg
      721 ggcggtgcgg cggtaggaga ggtccggcgc tagcgcggtg tccatcccgc ccagcgtctc
      781 cgccgcattt gccacatggg tcagcagggc ggcgcggaag gcgttatcgg gcaggtcggc
      841 cagattgacg cccgacgagg cgctgaaggc gacgaccagc ggcagccggt catcgctgtt
      901 gaggacgaca tagccgaccg gcgagaacgc cacggcatag acactgtagg agccgccgcc
      961 ttcctggaat ctgaccgtcg aggcgatggc gtttccagcc agacagccca tcaccggatg
     1021 gccatttatc cagatggata acctgtcgag cgcttcccgt tccgtcaagg gggcacctga
     1081 tgaaacgagc acaatgccca acacggcgca tgccaccctg aaggaagtgc cgagacgcac
     1141 ctgtaatgtg agttttttaa tcatggcctt tcgtgtcgtt ttcccgcgca cagagccaag
     1201 cgatgagctg ttccctgtcc tcaaaccctt tgtgggccac ttttgtctga cgtcggcatg
     1261 acgggcttcc gctgagaaac gaaccgtggc gaaggaacag atcgggcagt ctccagagat
     1321 agccgcccgc taaaccgaac caacgtactg tacggggata aatctgtcgc atttctgcgg
     1381 cagaccgaaa gagactcgcc agtccgatcc ggatccgttc caatcctttg gagcggataa
     1441 accgggagag ggaaggggtg agtttgatgc ttttcgcatc caacccgacc acaatttgtt
     1501 ccgcgacgtc caatgcctca cacatctcgg cggactcctc cgggattgcg gcccacatcc
     1561 ggcacgcctc ggacttgagc aggcgatcgg ccatgcgtag cacgaaggag atgccgtaca
     1621 ccgcgcccca ccgtgccctc tcgtgacgga atgccgtgtc cgagagcggc gcggcaaacc
     1681 gttcgcaaac cagacacagg tccagataaa ccttgagcgg cacagccatc cggtgcccca
     1741 gaatatggtg tgcgaggtat gcgacctgtc tttccggtgc aaaggtttgc accgggatcc
     1801 cgtgcaaccg gtcctccatg tttccgtgcc atacccaatc cgttgacggg gcttctcccg
     1861 ccgaaaccgg atggctgaac aaacgccggt ggagttccag agcgagcggc tcgccgcatt
     1921 tttggaagtg cgcgtgtttc acccgcctgt ccgtgacgtc gtaatcgggc gtcacatacc
     1981 cgatcgaccg gatgaccccg catgcctcgt taaaccgcgc ttccgggaca agcaggtcaa
     2041 tatcattcat cgtgcggcag ccgccctcca tatagatccg ctccgccagc cagacgcctt
     2101 tgagcgggat catctctatg cctgcggcat ggagtgccga aaacaactcg cgcgcctgtt
     2161 cagccacacg cgcgtatcgc ccggccgcct gtaggaaaac cgtccgccag aagtgcatca
     2221 tttcctctgg cggcaatgcc gcaggaggcc aggcacggat gaggggatag aggaacgttt
     2281 ccacctgatg catcgccgcc agacgataca ctgccggcca cgccagaggg tcgatttccg
     2341 cgacctgtcc gttcaccgca gcccgcatca cccgcaacaa gtcctcctgc gccggagtcc
     2401 gttcgggaag cttaggaaag gacccgctca taaagactcg cccattcgcc cttccgcgcc
     2461 cgtagcgcag ggatggttcc ctcttccacc agcacgccct cccgtagcac cagcacccgg
     2521 tcggcgacct cgatcacgcg acgccggtga ctcgccaaaa ccgccatccg gccaaccgtc
     2581 accgtttgca gccattggat cacctcatcc tgcgcacggt catccagcgc gcttgccggc
     2641 tcgtccagca acagcagatc cgcatcacgc aacgtggcgc gcgccaccgc gacacgctgc
     2701 cactgtccga ggctcggttc gattccttcg cgcaaccagc gccccaatgg ggtgtcgagt
     2761 ccccggggat ccgtccgggt cagggcatcc aagcccacac ggcgcacggt ttccgcaatg
     2821 cgtgcgtccg atgcgctgcc atgcggatcg ccgatccaga cattttcggc cagggagaac
     2881 tgatactgcc cgaaatcctg aaagagcgcg ccgatccgct gacgccacac aacggggtcc
     2941 acctcgcgta aatccacacc ctcaaacaga atccgtcccg ccgtcggatc gtaaaaccgg
     3001 cacatcagct ttagaagcgt ggatttgccg gacgcattcg cccccaccag cgcgatccgt
     3061 tcgccgggat gcagggtaaa actgatctcg cgcaataccg gtcgggaggt tccggggtag
     3121 gtgaaggata cacgctcaaa cacgaggcca tgacaccccg gtgcgggaag cgcgcgcggt
     3181 cgggacggcg tacgcacggt gtccggtttt gcgagcaggg tgtcaaagga ttcgagaaac
     3241 agtgcggact ggtagagttc ggcaagggtt cccgcaagcg ctccggtctg cgcctgtccc
     3301 cgctggaccg cctgcgcgta catgaccagc gccccgaggg aaagcgctcc cgccgccaaa
     3361 cgtccagcca tccaaagcag tagcactccc attgtggcca aagccagaaa ctgcatcgcc
     3421 aactcctggg tcaacaccag acggagcagg cgcaaccgcg ccgcacgcaa ccgcgaacgc
     3481 accgccgtga accggttacg gcaaaacgtg ccgtgtccca tcacccggat ctctttggcg
     3541 tacgcgccat ccgtgagcag atgatgcaca taaccggctt cgcgctccaa cggggcctgt
     3601 tcgcgacgcc a
//
LOCUS       DAOSRB010000261         3554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_23667_length_3554_cov_6.945699, whole genome shotgun sequence.
ACCESSION   DAOSRB010000261 DAOSRB010000000
VERSION     DAOSRB010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23667_length_3554_cov_6.945699"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLZ74_10505"
     CDS             complement(<1..519)
                     /locus_tag="PLZ74_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOR98789.1"
                     /translation="MKRIDFSNYLDALQQRRQPWQQGYYAMYSSVLGGVVRDPLLMQV
                     PLDDHLVHRGDGVFDTFKCVGRAAYNLEAHLQRLLRSASSIGLSWPDGIDGIRALTVE
                     TLAEAGREVCSGRVMISRGPGSFGVSPYESPAPALYIVVYAAGVPFMVRHPEGARVRR
                     SQVPVKPARFATV"
     gene            complement(744..3443)
                     /locus_tag="PLZ74_10510"
     CDS             complement(744..3443)
                     /locus_tag="PLZ74_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98790.1"
                     /translation="MAQRTAGCRKRVVLFTLVAGGLILSSQAAWIGAGTAGGDPDATD
                     INDANNWQSGVIDGDFRTVVSNVDLRLTADYTATNGLDFSQTASVLRHITISGTNTLT
                     LNAEIPYYNFNKGSTHVFLPSNTASTVTLKRGLTLSVPSGTWHVRGYGTLFIDAKVTG
                     KGKLNTGTGSGRTYIVLRNGTNDFRGGTSGDTGRYNFTSIGNSGVPSAVGASGDISPN
                     NAPVAYIGSRSRSTDRLFQFNHNGARLVNDSACGSLNMLGIVRPTNWGGNWLSPMSFD
                     GISSGETLVTNNIANGSSTSYARLCKLGSGTLRLTGSNTFFCTDSSVHQVELLGGTLI
                     ADYADDVTGAGSNRLFLAGHTVSYDNGHLVIRGKTGAGNTTWQEFGTNTIGVSNAIRS
                     NVLTVDGNGGDGTTVQLGLIDMPSSYSFLLIERLGNASVRTAEAIPSDNGSVRVTNGL
                     VMCADGTRANILVKDPDGRTGFAVQDENLGLVRHTGTIALTPDNATAVSHVSLASDLT
                     RTANLTFSTLDIDASANPVTLNLNGFSFQTDNSVVGRGIVINGSNPVAILGGAHGAQS
                     THIYNYGTDKFTWGLTNSSGCTLVSAGPGLTEITQPVLSSLYIVGGVTRLSCGRNYTE
                     GAISIHGDGVLEIGADLNGASDGDFSRWSGTSSGQIGFIEGGGFSAYGADRTVIMNDS
                     ATRVVTWGESRFMVDGKPFVLSSPYANATLIFANAVNLGERAREFRVRNGSAAVDACL
                     TNRIYGIRVAALVKSGDGTLELKGPQDYPGECSVIAGGLKLGADDVFTGGVNALVLSG
                     ATLDAGTFRNTFNTLELLTDSVIEAGDGSATLAFADSSSETWTGTLTINGKLTAKTLR
                     FGTDGKGLSAAQLASITNRDRPVTLDEQGYLRQIPAGTVLLFF"
BASE COUNT          676 a         1103 c         1103 g          672 t
ORIGIN      
        1 caccgtggcg aagcgcgccg gcttgaccgg cacctgactg cgccgcacgc gggcgccttc
       61 cggatgccgg accataaacg gcacccctgc cgcatacacc acgatgtaaa gcgccggtgc
      121 gggcgattcg tagggactca cgccgaaact gcccggtccg cgcgaaatca tcacccggcc
      181 cgaacagacc tcgcgccccg cctcagccaa cgtttccacg gtaagcgcgc ggattccgtc
      241 gatcccgtca ggccaggaca ggccgatcga cgatgccgaa cgcaacagcc gctgcaagtg
      301 cgcctccaga ttgtaggccg cgcgccccac gcatttaaac gtgtcgaaga ccccgtcacc
      361 gcgatgcaca aggtggtcat ccagcggcac ctgcatcaag agcgggtccc gcaccacccc
      421 gcccagcaca ctggagtaca tggcgtagta tccctgctgc cacggctgac gccgttgctg
      481 cagcgcatcg aggtaattcg agaaatcaat ccgtttcatg tccatccttc cgcgcggcgc
      541 ggccgcgtcc ggttcaataa gggatggagg atagcatatc cggtgccggc cgccaacagc
      601 gcggaccggg aaagggaaga accggggcgc atcccctttt cagtcgggaa cgcggccgtc
      661 cccgccagta cggcggatca gtcaggtcgg actgaccagt cagatccgag gcgcccctgc
      721 cggtcgtcgc acgatggtca aatctaaaag aagagcagaa ccgtgccggc gggtatctgg
      781 cgcagataac cctgctcgtc cagagtgacg gggcggtcgc ggttggtgat ggacgcgagc
      841 tgggcggccg agagtccctt gccgtcggtg ccgaagcgca gagtcttggc ggtcagcttg
      901 ccgttgatcg taagcgtgcc ggtccaggtc tcagagctgg agtcggcaaa ggcgagcgtg
      961 gctgagccgt cgccggcctc gatcacgctg tcggtcagca attccagcgt gttgaacgtg
     1021 ttgcggaacg tgcctgcgtc gagagtcgca ccgctcagca cgagcgcgtt caccccgccc
     1081 gtgaacacgt catccgcgcc gagtttcaac ccaccggcaa tgaccgagca ttcccccgga
     1141 taatcttgcg ggcctttcag ctccagcgtg ccgtcgccgc tcttgaccag agcggccacg
     1201 cggatgccgt aaatccggtt ggtcaggcag gcgtccacgg cggccgagcc gttccgcaca
     1261 cggaactcac gggcgcgctc gcccagattg accgcgttcg caaaaatcaa ggtggcgttg
     1321 gcatagggcg aacttaagac gaacggtttg ccgtcaacca tgaaccttga ttcaccccat
     1381 gtgacgaccc ttgtggcgct gtcgttcatg atgacggttc gatccgctcc gtaggcgctg
     1441 aaaccgccgc cctctataaa accgatctgg ccactcgaag ttccggacca gcgcgaaaaa
     1501 tcgccgtcgg atgcgccgtt cagatccgcg ccgatctcca gaacgccgtc gccatggatg
     1561 gagatggctc cttcagtgta gtttcgcccg caggaaaggc gggtgacacc tccgacgatg
     1621 tacagtgaac tcaaaacagg ctgggtgatc tccgtcaggc cggggcctgc ggaaacgagc
     1681 gtgcagccgc tgctgttggt gaggccccac gtgaatttgt ccgtgccgta attatagata
     1741 tgtgtgctct gcgcaccgtg cgcaccgccg aggatcgcga ccgggttgga cccgttgatc
     1801 acgatgcccc ggccgaccac cgagttgtcg gtctggaacg agaagccgtt gaggttcagc
     1861 gtgacgggat tggcggaggc gtcaatatcg agcgtcgaga atgtgagatt cgccgtgcgc
     1921 gtgagatcgg acgcgagcga gacgtggctg accgcggtcg cgttgtcggg ggtcagcgcg
     1981 atcgtgccgg tgtgacgcac gagcccgagg ttctcgtcct ggacggcgaa ccctgtccgt
     2041 ccgtccggat ccttgaccag aatgttggcg cgggtgccgt ccgcgcacat gaccagaccg
     2101 ttggtgacgc ggactgaacc gttgtcggag gggatcgcct cggcggtacg caccgaggca
     2161 ttgccaagac gctcgatgag cagaaaactg taactgctcg gcatgtcgat gaggccaagc
     2221 tgaacggtgg tgccgtcgcc gccgttgccg tcaaccgtca ggacgttact ccgtatcgcg
     2281 ttactgacac caatcgtgtt ggtcccgaac tcctgccatg tggtgttgcc cgcgccggtc
     2341 ttgccccgga tgacgagatg gccattatca taactgaccg tgtgcccggc cagaaacaat
     2401 cggttgctgc ccgctcccgt cacatcgtcc gcgtaatcgg cgatcagcgt gccgccgaga
     2461 agttcgacct gatggacgct gctgtcggtg cagaagaagg tgttactccc ggtcagcctc
     2521 agcgtgcctg atcccaactt gcaaagtcta gcgtaacttg tcgagctgcc gttcgcgatg
     2581 ttgttggtga cgagagtctc gccggaggaa atgccgtcga aactcatggg cgacaaccag
     2641 ttgccgcccc agtttgtcgg acggacgata ccgagcatat tcaggctgcc gcaggcgctg
     2701 tcgttgacga ggcgcgcccc gttgtgatta aactggaaca aacggtcggt ggagcggctg
     2761 cggctgccga tgtaggctac gggcgcgttg ttagggctga tgtcgcccga tgcgcctacg
     2821 gcggaaggga cgcccgaatt accgatggac gtgaagttgt agcggcccgt atcgccgctg
     2881 gttccgcccc taaaatcgtt ggtgccgtta cgcaggacaa tataggttct cccgctcccg
     2941 gtgccggtat tgagcttccc tttcccggtc accttcgcgt cgatgaacag tgtgccatat
     3001 cccctcacgt gccatgtgcc tgacggcacg ctaagcgtca gcccccgctt taaggtcacc
     3061 gtgctggccg tgttggaggg aaggaacaca tgtgtgcttc ccttgttgaa attgtaatag
     3121 ggtatctccg cgttcagcgt gagggtgttg gtgccgctga tcgtgatgtg acggagaacc
     3181 gaggcggtct gcgaaaagtc cagcccgttc gtcgccgtgt agtcggctgt cagccggaga
     3241 tcgacattcg acacgacggt tctgaaatcg ccgtcaatga cgccagactg ccagttgttg
     3301 gcatcgttga tatcggtggc gtccgggtcg ccgcctgcgg tgcccgcacc gatccaggcc
     3361 gcctgcgacg acagaatcag tccgcccgca accagcgtga acagaacaac ccgtttacga
     3421 caacccgctg tccgttgtgc catgaccaac ctccactttt tttcacaacc accccatggg
     3481 atgccccggg aacatcccgg ttcccatgtt tgacgcttat tatgcggaaa agaggcgaaa
     3541 agagtcaacc ctgt
//
LOCUS       DAOSRB010000262         3536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_23801_length_3536_cov_8.169204, whole genome shotgun sequence.
ACCESSION   DAOSRB010000262 DAOSRB010000000
VERSION     DAOSRB010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23801_length_3536_cov_8.169204"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1748)
                     /gene="aroB"
                     /locus_tag="PLZ74_10515"
     CDS             complement(120..1748)
                     /gene="aroB"
                     /locus_tag="PLZ74_10515"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOR98791.1"
                     /translation="MHLFLYGPPGSGKSTIGRLLAQRLDLPFTDLDAVIEASAKQRIR
                     DIFACEGEAGFRTRETRALAETIAAPPAVIALGGGALLKGENRAMAEAAGEILCFDAD
                     AATLERRVACAPGVRPLLNVPSSDCATPPAPGQGKPIATLLSERSAHYASFPLRVKVS
                     DRPPEETADAAQTALGLFRVSGMGSAYRVRVGNGLLDTLGACFRELDCGDRTVIVGDT
                     HTLPLYGERAAAPLRALGVTVSLVPIPAGEDSKTIDTVAALWSAFLKAGIERGHAVIA
                     LGGGVVGDLTGFAAATWLRGVRWIGLPTTLLAMVDSSLGGKTGADLPEGKNLIGAFHP
                     PALVLADTATLATLPGRDLRCGLAEAVKHAVIGDPGLLDTLPRFAFCKDGDASIPCAR
                     LSDAAWLAPFVARAMAVKIRIIREDPFEKGFRAALNLGHTIGHGIEKATHFAVRHGEA
                     IAIGTVLEARLAEELGLAQPGLAGKLANLFSSLGLPVVPPPDIDPAEVLAAITLDKKK
                     TDGKVRFALPVGLGEVRTGVTVDDASLTRVLKRG"
     gene            2104..3369
                     /locus_tag="PLZ74_10520"
     CDS             2104..3369
                     /locus_tag="PLZ74_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98792.1"
                     /translation="MNAHHDQPGNVALDTHVADLTITSAERDVLRKLGERVMSLSRRQ
                     IEAEKKVLWTRQNQLQPSRPLILCDPEFSWREIIPDTSLQCEHALARHWERRLRKEIF
                     WGEKMGDDRVVESRFTVGWVYRRTFWGPDASSIHPQSTWSDLNDLSGLQFQTITVDRD
                     ATTDVVHLAREVFDRVLPVKLREQWYWSLGLTRELIKLRGFEQFMRDIRENPAGVHNL
                     MAFLRDGTLDLITFLEQAGLYSLNNEGDYIGSGGFGWTTQLPAKDYQGTARAKDLWAL
                     FESQDTRDLSPELFDEFVLQYQKPVMERFGLVCYGCCEPISERWHLLQNVPNIRRVSV
                     SPTCNRAKMAEALQDRYVYSCRPNPAILAASSFDADAARKEIRDILEKAKGCHLEIIM
                     KDHDTIHNDPARAIAWCQIAREEIARYYQ"
BASE COUNT          660 a          974 c         1192 g          710 t
ORIGIN      
        1 gatcccgatg gcgatagcga tggcgccgca atggaaccct gaaacgcatg accttcgcac
       61 acccctgtca cgaaccgatt accttgccaa caggcgtgtg gggtgtttat atcaggcggt
      121 cagccgcgtt tcagcacgcg tgtgagggac gcgtcatcga cggttacgcc ggtgcggact
      181 tcgccgaggc cgacgggcag ggcgaagcgc actttgccgt ctgtcttttt cttgtccagg
      241 gttatcgctg ccagcacttc ggcagggtcg atatcgggag gcggaacgac ggggagacca
      301 aggctggaga agaggttggc gagtttcccg gccagcccgg gttgagcgag gccgagttcc
      361 tcggccagac gggcttcgag cacggttccg atggcgatgg cctcaccatg ccgcacggcg
      421 aaatgcgttg ctttctcgat gccgtgaccg atcgtgtgtc cgaggttgag cgctgcgcgg
      481 aacccttttt caaaggggtc ttcccggatg atgcggatct tgacggccat ggcgcgggcg
      541 acgaacggcg cgagccaggc agcgtccgag agcctggcgc agggtatgga ggcatctccg
      601 tctttgcaga aggcgaaacg tggcagggta tcgagcaggc cggggtcgcc gatgaccgcg
      661 tgtttgacgg cttcagccag gccgcagcgc aaatcgcggc ccggcaaggt ggcgagtgtg
      721 gcggtgtcgg ccaacaccag cgcgggcggg tggaacgcac cgatcaggtt tttgccttcg
      781 ggcaggtcgg cgccggtctt gccgccgagg ctggagtcga ccatggcgag cagcgtggtg
      841 ggcaggccga tccagcgcac gccgcgcaac caggtggcgg cggcgaaccc ggtgagatcg
      901 ccgaccacac cgccgccgag cgcgatcacg gcatgaccgc gctcgatacc ggccttgagg
      961 aaagcagacc agagcgcggc gacggtgtcg atggttttgg agtcctcgcc tgcgggaatc
     1021 ggaacaagcg agaccgtgac gccgagtgcg cggagcgggg cggcggcacg ctcgccgtag
     1081 agcggcaagg tgtgggtgtc gccaacgatc acggtacggt cgccgcagtc gagttcgcgg
     1141 aaacacgcgc ctagggtgtc gagcagaccg ttgcccacgc ggacacggta ggcggaaccc
     1201 atgcctgata cgcgaaagag gccaagcgcg gtttgcgccg catccgccgt ttcttcgggc
     1261 ggccggtcag acaccttcac gcgcagggga aaagaggcgt aatgggccga acgttcggac
     1321 agtaatgttg cgatgggttt gccttgtccc ggtgcgggcg gggtcgcgca gtcggatgac
     1381 gggacgttca aaagcggccg cacaccgggg gcgcacgcga cacgtctctc cagtgtggcg
     1441 gcatcggcat cgaagcagag tatctcaccc gcggcttccg ccatcgcccg gttctcccct
     1501 ttcaacaggg caccgcctcc caaggcgatc acagcagggg gggctgcgat cgtttcggcg
     1561 agtgcgcgtg tttcgcgggt acggaacccg gcctcgccct cacaggcgaa aatgtcgcgg
     1621 atacgttgtt tggcggaggc ttcgatgact gcgtcgagat ctgtgaaggg caggtccagg
     1681 cgctgcgcca acaggcgtcc gatggtggac ttaccgcttc ccggggggcc gtacagaaaa
     1741 aggtgcatga tatgccgtgg gtgaggggtg gaccgtattc gtgtttccgt ccggagggga
     1801 aggcttcccc ccctgccgga tcgaacaccg cgcatcttat gtggcctgcc gttgaaaaac
     1861 aagttgtttc tcacacgaag gtttttgagg gcatggctcg ggggattttt cggaatcgtc
     1921 cttctccggc caccgattcc ccgctggaca attccggttt gcgcctttat acttcgggtc
     1981 attcaacatg ccccttttag tgtgggtgtg cggaaacaaa tgcttcggcg gaccgtcacg
     2041 acaccatgcc gcccggggcg atctgatcgg aatcgggtgt cgtaagaaaa ggagggttcg
     2101 gttatgaatg cccatcatga tcaaccgggc aatgtcgcac tggacacgca tgtcgcggac
     2161 ctcaccatca catcggcgga acgcgatgtg ttgcgcaagc tgggggagcg ggtcatgtcg
     2221 ttgtccaggc ggcagattga ggcggagaaa aaggtccttt ggacgcggca gaatcagttg
     2281 caaccctcgc ggccgctgat cctgtgtgat ccggaattca gttggcgtga gatcattccg
     2341 gacacgtcat tgcagtgcga acacgcgctc gcccggcatt gggagcgccg cttgcgcaag
     2401 gagattttct ggggcgagaa gatgggggat gaccgggtcg tcgagagccg gttcacggtc
     2461 ggatgggttt atcgccgcac gttttggggg cctgatgcct catcgatcca tccgcaatcg
     2521 acatggagcg atttgaatga tttgagcggg cttcagttcc aaaccatcac tgttgaccgg
     2581 gatgccacga ccgacgttgt gcatctggcg cgtgaggttt tcgaccgtgt cctgccggtg
     2641 aagctgcgcg agcaatggta ttggtccctt ggcctgaccc gcgagttgat caaactgcgc
     2701 ggtttcgagc agttcatgcg ggatatccgc gaaaatccgg ctggagtgca caatctgatg
     2761 gctttcttgc gtgacggcac gctggacctc atcaccttcc tcgaacaggc cgggctgtac
     2821 agtctcaata acgaagggga ttacatcggt tcgggcggat ttggctggac cacgcaactg
     2881 ccggccaagg attatcaggg gacggcacgt gccaaagatc tctgggcgct gttcgaaagt
     2941 caggacaccc gggatctgtc acccgaactg tttgacgagt tcgtgctgca ataccaaaag
     3001 ccggtcatgg agcgcttcgg gctggtgtgt tatgggtgct gcgagccgat cagcgagcgg
     3061 tggcatttgt tgcaaaacgt gccgaacatc cggcgggtca gcgtgtcgcc gacctgtaac
     3121 cgggcgaaga tggcggaagc gctgcaggat cgctacgtgt actcttgcag gcccaacccc
     3181 gcgattctcg ctgccagctc gtttgatgcg gatgcggcgc gcaaggagat ccgtgatatt
     3241 ctcgagaagg ccaagggttg ccacctcgaa atcatcatga aggaccacga tacgattcac
     3301 aatgatccgg cccgcgcgat cgcgtggtgc cagatcgccc gagaggaaat cgcccgatac
     3361 tatcagtgaa cctaaaaaga gcagttgaac cttcaacgct cttgggtttc tgggtttcag
     3421 ggtttctggt ttcagggttt ctgggtttct ggtttcaagg tttcagggtt tcagggtttc
     3481 tgggtttcag ggtttctggt ttcagggttt cagggtttca gggtttcatc caaatc
//
LOCUS       DAOSRB010000263         3527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_23898_length_3527_cov_7.387097, whole genome shotgun sequence.
ACCESSION   DAOSRB010000263 DAOSRB010000000
VERSION     DAOSRB010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23898_length_3527_cov_7.387097"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..2540)
                     /locus_tag="PLZ74_10525"
     CDS             complement(174..2540)
                     /locus_tag="PLZ74_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOR98793.1"
                     /translation="MMNITRRAFLGSAAMFTAAAGLRGENVPPVTPFDSAAYTALRKQ
                     LRAFYPDECRGVAKDPRQPESEKKIAAEVRAWANANPGFDALDIRRESYRAMTRHFVP
                     FLFTESPFYFEAGVNGGWSGARPARVVNAICGRFYKEKNLIPDEAFRLLRKRNGERMA
                     LCCGPFVDDMHHVPPIRVVLAKGFGGIRADVAAALETCPKNDPYGRKLLETALVGFDT
                     LRDLQLAFAREAQKRLEDPALDATGRRHFTRIAESAQRCPWEPPQTFFEGLNTLWFIR
                     EILGYTDGLMIYALGRPDAWLYDLYRADLAAGRLTEAEAADLVARFLITADCHHDGMI
                     PVDSYSDHEMEIPMSLGGCDKHGKPVYNELTRLFLDAHIGTDCVFPKLHCRIASDSPQ
                     EYLAKIGGMLTKGHAVFALLNDDRYIPQYIRQGFPVEDAREYTGAGCWNGYIDSVMDV
                     DGANYVSVMRTFELAIHRDPKVEREARIRIDPLDGVTSFEEARNIAYRNFIRFFRDVM
                     SEYTRYGAQNAKVFPHPVYSMCLRGGIQSRRDTTEGGIPARPRVITLGFIGNVVDSLL
                     AIKHLCFDTKTCTLPELLAAVRSNWSGPRGAELRKAAFAGPYWGDGTEQTCGEMAWWI
                     RRIHDDIEGFANDQGDAYRLAIYTYREFMYWGNATKATPDGRRDGDRLAQGFSPSEYR
                     CKEGVTTVLNAIGTLPHECLFASNANLTFDKTAMSPETFAAVFRVYAKKCGHLLQPNC
                     NSVEQLLEAQKHPERYQYLIVKVCGFSARFVALSKRWQDEVIQRHRLR"
     gene            complement(2662..>3527)
                     /locus_tag="PLZ74_10530"
     CDS             complement(2662..>3527)
                     /locus_tag="PLZ74_10530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOR98794.1"
                     /translation="TPDSLFDLASVGKTFTTSLCAMLVADGKLELDAPFTKYLPEHVL
                     AKENCAITVRDLAMHIGGFDNSKPYILKEPNAAAFDAALFKKRPVRARGIKYEYACSN
                     FIYLGKIVERLTGLELEAAAKKMLWEPLGMKDTYWHDIPGHPRAVQVFTNNRPAIGMR
                     SDEQARSYPSATGNGATFSSISDMLLFADDLLHRKHFKKAYYDLLFIPRFEKDGSRRS
                     FGWDMSAKIVPNGWSRATIEHGGHTGITIAIDPVNRYSGIVMTNRTGGWWQGYECRMR
                     ILSLMPGCPVS"
BASE COUNT          697 a         1057 c         1070 g          703 t
ORIGIN      
        1 ggaactgctc ctgcattaaa agaacggaaa cattccggct cccctgattg atgcctatca
       61 tgcgggcaag gggtgaaaag agtcaacccc aaaaaatcga taatccgcgc gacagcatgg
      121 ccaccctagc attccccgcg ctgtcgcgtt aaacaatccc atctggcgtt tcctcatcgc
      181 aggcgatggc gctggatcac ctcgtcctgc cagcgtttcg agagtgccac gaagcgtgcg
      241 gagaacccgc ataccttgac gatgagatac tgatagcgct cgggatgctt ctgcgcttca
      301 agaagttgct ccaccgagtt gcagttaggt tgcagcaggt ggccgcactt tttcgcgtac
      361 acgcggaaca cggcggcgaa cgtctccgga ctcatggcgg tcttgtcgaa cgtgaggttg
      421 gcgttggaag cgaacaggca ctcatgcggc agcgtgccga tggcgttgag cacagtggtc
      481 acgccctcct tgcagcggta ttcgcttgga gagaagccct gcgccagacg gtcaccgtcc
      541 ctgcgtccgt caggggtcgc cttggtggcg ttgccccagt acatgaactc gcggtaggtg
      601 tagatggcca gccggtaggc gtcgccctga tcgttggcaa acccttcgat gtcgtcatga
      661 atccgtcgta tccaccaagc catctcgccg catgtctgct ccgtgccgtc gccccagtac
      721 ggtccggcga acgccgcctt gcggagttcc gcgccgcgcg gaccggacca gttgctccgt
      781 accgccgcga gcagttccgg caacgtgcag gttttcgtgt cgaagcagag gtgcttgatc
      841 gcgaggagcg agtcgaccac gttgccgatg aagccgagcg tgatcacacg cggacgcgcg
      901 gggatgccgc cttcggtggt gtcgcggcgg ctctggatac ccccccgcag acacatcgag
      961 tagaccgggt gcggaaagac cttggcgttc tgtgcgccgt aacgcgtgta ctcgctcatc
     1021 acgtcgcgga agaaacggat gaagttgcga taagcgatgt tgcgcgcctc ttcaaagctc
     1081 gttacgccat cgaggggatc gatgcggatc ctggcttccc gctctacctt cggatcgcgg
     1141 tggatcgcca gctcgaaggt gcgcatgacc gaaacgtagt tggcgccatc cacatccatg
     1201 acggagtcaa tgtagccgtt ccagcagccg gcaccggtgt actcgcgggc gtcctccacg
     1261 ggaaaacctt gcctgatata ctgggggatg tagcggtcgt cattcaggag cgcaaagacg
     1321 gcgtgaccct tggtgagcat gccgccgatc ttggccagat actcttgcgg cgagtccgaa
     1381 gcgatgcggc agtggagctt cgggaagacg cagtccgtac cgatgtgcgc atcgagaaag
     1441 aggcgcgtca actcgttgta gaccggcttg ccgtgcttgt cgcaaccgcc gagcgacatc
     1501 ggaatctcca tctcgtggtc ggagtaggag tcgacgggaa tcatgccatc gtgatggcag
     1561 tcggcggtga tcagaaagcg ggccaccaga tccgcagcct ccgcttcggt gaggcgtccg
     1621 gcggcaaggt cggcgcgata gagatcgtac agccatgcgt cgggacgccc caacgcatag
     1681 atcatcaggc catcggtata acccaggatc tcgcggatga accagagcgt gttgagccct
     1741 tcgaaaaatg tctgcggcgg ctcccagggg cagcgttgcg cgctctcggc gatgcgcgtg
     1801 aagtggcggc ggccggtggc atcaagcgcg ggatcttcca gtcgcttctg cgcctcgcgg
     1861 gcgaaggcaa gttggaggtc gcgcagggtg tcgaatccga ccagcgccgt ttcaagcaac
     1921 ttccgtccgt acggatcgtt cttggggcac gtctccaacg cggccgcaac atcggcgcgg
     1981 atgccgccga aacccttggc gaggacgacg cggatcggcg gcacatggtg catgtcgtcc
     2041 acgaacggcc cgcaacagag tgccatgcgt tcgccgttgc gttttctgag caggcggaac
     2101 gcctcgtcag ggatcaggtt cttctctttg tagaagcgcc cgcatattgc gttcaccacg
     2161 cgggccgggc gcgccccgct ccagccgccg ttgacgccag cctcgaaata gaacggcgac
     2221 tcggtgaaaa ggaacggcac gaagtggcgc gtcatcgccc ggtagctttc gcgccggata
     2281 tcgagcgcgt cgaacccagg gttcgcgttc gcccaggccc gcacctcggc agcgatcttc
     2341 ttttcacttt ccggctggcg tggatccttc gccacgccgc ggcattcgtc aggatagaac
     2401 gcccgcaatt gctttcgcag tgccgtatac gccgcagaat cgaaaggtgt cacggggggg
     2461 acgttctcac cccgcaaccc cgcagccgcg gtgaacattg cggcgctacc caaaaaggcg
     2521 cgtcgtgtga tgttcatcat cgtcacactc ctctctccca ataaacccat attgtttatg
     2581 agggggtggg aaaagtcaat cccgcctgcg aaaaagagca tgtgcgcatc acccttttac
     2641 acaataagaa agactcatcc cctagctcac tgggcagccc ggcatgagcg agaggatgcg
     2701 catacggcat tcgtaaccct gccaccagcc gccggtgcgg tttgtcatca caatgcccga
     2761 atacctgttc acgggatcga tcgcgatcgt gatgcccgtg tgtccgccat gctcaatggt
     2821 ggcacggctc catccgttcg gtacgatctt ggcactcata tcccagccga aactgcgtcg
     2881 tgaaccgtct ttctcgaagc ggggtatgaa gaggagatca tagtacgcct tcttgaaatg
     2941 cttacggtgc agcaggtcgt ccgcgaaaag caacatgtcg gaaatggatg agaacgtcgc
     3001 gccattgccc gtcgcgcttg gataactacg tgcctgttcg tcactacgca tgccgatcgc
     3061 gggccggttg tttgtgaaca cttgaacggc acgcggatga ccggggatgt cgtgccaata
     3121 ggtgtctttc atgcccagcg gctcccacag catctttttt gccgctgctt cgagttccag
     3181 tcccgtgaga cgctccacaa tcttgccgag atagataaag tttgaacacg cgtattcata
     3241 tttgatgccg cgtgcccgta ccggacgctt cttgaagagc gccgcatcga aagcagcggc
     3301 attcggctcc ttgaggatat agggcttgga gttgtcgaac ccaccgatgt gcatcgcaag
     3361 gtcacgcacc gtgatggcgc agttctcttt tgcgaggacg tgctcaggca agtacttcgt
     3421 gaacggcgca tcaagctcca gcttaccatc cgccacaagc atcgcgcaga ggcttgtcgt
     3481 gaaggtcttg cccacactcg cgaggtcgaa aagcgaatcg ggcgtca
//
LOCUS       DAOSRB010000264         3501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_24125_length_3501_cov_7.550784, whole genome shotgun sequence.
ACCESSION   DAOSRB010000264 DAOSRB010000000
VERSION     DAOSRB010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24125_length_3501_cov_7.550784"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLZ74_10535"
     CDS             complement(<1..942)
                     /locus_tag="PLZ74_10535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR98795.1"
                     /translation="MPENRILLVSSDRTEWFEEASDNEQFERFPYSVYAILFNLSKKG
                     WQTCRAGWKTSSKLKALAQRIDTFQPSIIYTYGSTVALNPLLCRRFFCKWKSFKVIHG
                     WDDLYGDIWHEIAGYPGRILMNIMEKLIVTRSDHVVTLSRFLQQRGKAWGVDCHYIPN
                     GADIPVFDHAACSIRLEGDMNLVYTGDQARWKRTADICEAMRHVPKNIKLYLTGRRYP
                     YLDKYASDNCIFLGYVSRNDQLCVMDQADVLVSTADQDCNAKLQEYLRFKKPILGYDG
                     RMNLFFKNGENALLTRDYPNAILTLYNAPTLRTTLAEN"
     gene            complement(1124..>3501)
                     /locus_tag="PLZ74_10540"
     CDS             complement(1124..>3501)
                     /locus_tag="PLZ74_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOR98796.1"
                     /translation="SLNSGLQLYYPFTSATEGATDASGNGHDGVLHGTTWQDDGRPSG
                     SRRLGSGSYIDVGTEVNFAAWEQYSISLWFKRDLTPIDATGYGDKLLCKTDWYSDQHL
                     RMLPGGSLHFSVTTGGGSYPSAGYTVDCRENFQDNMWHHLVVVRSNNLATLWIDGTQK
                     DVVSSAISITGNAKPLYLGYSPSGDTYQRRYWPGNLDELRIYDRALAPHEIDELLHGE
                     TRPLTVAFDVNGGEQAPTNTVVIMGAPYGELPQPSRTGYTFGGWFASTGEVTFVIAAD
                     TLVTLAEDHTLTAVWTPNTYTVAFDADGGKVAPVSKTVTFDAAYGVLPTPVAPPASRL
                     AWLFGGWMAVNGQLVTDATLVATASDHTLTAVWTKPAPLPDPEASPTFTSEAPYYGYL
                     YRTGDVGGQAASSVEGTIEFNVSAATGKLSAKAVTRRGPLNFRAPTWDAGEADGTHLV
                     TLKLRSGETLALSVRGNRAWGMLSGGSVDGELLVECARNRFRDRTDAEAQAVLTDFKG
                     LYTAALPTCGPADDDVGAVPQGAGYLSLTLSTYGRVKFAGVLADGTSVSMAAQALLID
                     GCGGWVCVPLFSPLYGRKGWIGGLVWLDPETGAAITDRELGWTIRWDRARGDAQDFSL
                     LLDLCGGRYAKPPALAPAYRFSVDIEQVPLFYPGGSALFAEEALPINLPVAVSGTRMT
                     MVRGRQPQKTDGAYNYGGENCAQAKLSFSASTGIYKGTFSLYYDYISGNRPRHKKVTV
                     QYAGILCPVRGAAFADAPAGMGHCLVPDNSTGPTLKRSFLNMLHEGAVPAL"
BASE COUNT          686 a          967 c         1118 g          730 t
ORIGIN      
        1 gttttccgcg agcgtggtgc gaagcgtcgg ggcgttgtac aacgtcagga tggcgttcgg
       61 atagtcacgg gtcaacaggg cgttctcgcc attcttgaag aagaggttca tccgaccgtc
      121 gtaacccaag atcggttttt tgaaccgcag gtactcctga agcttggcgt tacagtcttg
      181 atcggcggtg gagaccaaca cgtcggcctg atccatcacg cagagttggt cgttccgcga
      241 gacataaccg aggaagatac agttgtcgga ggcgtattta tcaagatagg ggtaacggcg
      301 tccggtcagg tacagcttga tgtttttggg gacatggcgc atggcttcgc agatgtcggc
      361 ggtccgtttc cagcgcgcct gatcgccggt atataccaga ttcatatcgc cttccaatcg
      421 gattgaacat gctgcgtggt caaagactgg gatgtcggca ccgttcggga tgtagtggca
      481 atcgacaccc caagcctttc cgcgctgctg caagaagcgg ctgagcgtga ccacatgatc
      541 ggagcgggtg acgatcagct tttccatgat gttcatcagg atccgtcccg gatagcccgc
      601 gatctcatgc cagatgtcgc cgtacagatc gtcccatccg tgaatgacct tgaacgattt
      661 ccacttgcag aaaaaccggc ggcagagcaa cggattaagc gcaacagtgg atccgtatgt
      721 gtagatgatc gagggttgaa atgtatcaat tcgttgtgcg agggccttca gtttggaact
      781 ggttttccag ccggcacgac aggtttgcca ccctttttta ctgaggttaa acaagatggc
      841 ataaacactg taagggaagc gttcaaactg ctcgttgtca gaggcttctt caaaccactc
      901 cgtgcgatcg cttgaaacaa gcaaaatcct attttcgggc atggggcacc tactccttgt
      961 tacgtgtcat taatatcaga tttcggttac gttgataaga cctgccggaa aaagaattga
     1021 ccttgaaata aagaagggga tccgtgttgg gttcccccaa cagtcccctt cattgtttta
     1081 ctggcttata cggccgctca aacacaaacg tagaggcgaa cacctacaat gccggcacgg
     1141 ctccttcatg tagcatgttc aagaacgagc gcttgagtgt ggggccggtc gagttgtccg
     1201 gcacgagaca gtggcccatg ccggctgggg catccgcgaa agcagcgcct ctgacggggc
     1261 agaggatgcc cgcgtattga accgtgacct tcttgtgacg tggacggttc cccgagatgt
     1321 agtcgtagta gaggctgaac gtacctttgt agatgccggt ggacgccgag aacgagagtt
     1381 tcgcctgtgc acaattctcg ccgccgtagt tgtaggctcc gtccgtcttt tggggctggc
     1441 gtccgcggac catggtcatg cgggtgccgg agaccgctac gggcaggttg atcggcaacg
     1501 cttcttctgc gaagagggcg ctgccgccgg gatagaacag gggcacctgc tcaatatcca
     1561 ccgagaatcg ataggcgggc gcgagcgcgg gcggtttggc atagcggccg ccgcagaggt
     1621 ccagcaagag ggaaaagtcc tgtgcatcgc ctcgtgcgcg gtcccagcgg attgtccagc
     1681 cgagttcgcg gtcggtgatg gccgcaccgg tttcgggatc aagccagacg agcccgccga
     1741 tccaaccctt gcgcccatag agcggggaga agagcggcac gcatacccat ccgccgcagc
     1801 cgtcgatgag cagcgcctgt gcggccatcg atacgctggt gccgtcggcg agcacaccgg
     1861 cgaacttgac ccgcccgtac gtactgaggg tgagtgagag gtagcctgca ccctgcggta
     1921 ccgcgccgac gtcgtcgtcg gccgggccac acgtcggcag agccgccgtg tagaggccct
     1981 tgaagtcggt caggacggct tgcgcttcgg cgtcggtgcg gtcgcggaag cggttgcgtg
     2041 cgcactcaac caaaagttcg ccgtcgaccg agccgccgct gagcatgccc caagcgcggt
     2101 tgccacgcac ggagagggcg agtgtctcgc cgctccgcag cttcagggtg acaagatgcg
     2161 ttccgtccgc ttcgcctgcg tcccacgtcg gggctctgaa gtttagtggc cctctgcgtg
     2221 tgacggcctt ggcggagagt ttgccggtag ccgcggaaac gttgaactcg atggtaccct
     2281 cgaccgagga ggccgcctgc ccgccgacat cgccggtgcg gtagaggtaa ccgtaatatg
     2341 gtgcctcgct ggtgaaggtc ggtgacgcct ctggatcggg cagaggggcg ggtttggtcc
     2401 acacggcggt cagggtgtgg tcggatgcgg tcgctacaag ggtggcgtcc gtcacaagct
     2461 ggccgttcac agccatccag ccgccgaata gccacgccag gcgtgacgcg ggcggcgcga
     2521 ccggtgtggg cagtacgccg taggcggcat cgaacgtgac cgtcttggag accggagcga
     2581 ctttgccgcc gtcagcgtca aaggcgacgg tgtaggtgtt gggcgtccag acggcggtca
     2641 gcgtgtggtc ttccgcgaga gtgaccagcg tgtccgcggc gatgacgaaa gtgacctcgc
     2701 cggtagatgc gaaccagcct ccgaaggtgt agcccgtgcg tgacggctgc ggcagttcgc
     2761 cgtacggggc gcccatgatg acgaccgtgt ttgtaggggc ttgctcgccg ccgttcacat
     2821 caaaggcgac tgtcaacggc cgtgtctctc cgtgcagaag ctcgtcgatc tcatgcggag
     2881 ccaaggcacg gtcgtaaatc ctgagttcat caaggttgcc gggccagtag cgccgttgat
     2941 aggtgtcgcc ggacggtgag tagccgagat agaggggctt cgcgttgcct gtaatcgaaa
     3001 tcgcactcga aacaacatct ttttgtgttc cgtcaatcca cagcgtcgca aggttgttgc
     3061 tccgcacgac gacgaggtgg tgccacatgt tgtcctgaaa attttcccgg cagtcgactg
     3121 tgtatccggc agaaggataa gatccaccgc cggttgtcac tgagaagtga agagacccgc
     3181 cgggtagcat gcggaggtgc tggtctgaat accagtcggt cttgcacagc agtttgtcgc
     3241 catatccggt agcgtcaatc ggggtcaagt cgcgtttgaa ccacaggctg atggagtact
     3301 gttcccatgc agcaaaattc acctctgtgc cgacgtcgat gtaacttcct gagccgagcc
     3361 ggcggctgcc ggagggcctc ccgtcgtcct gccaggttgt gccgtgcaac acgccgtcgt
     3421 gtccgttccc gctggcatcc gttgcacctt ccgttgcgga tgtgaacgga tagtaaagtt
     3481 gcaacccgct gtttaaggat g
//
LOCUS       DAOSRB010000265         3466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_24425_length_3466_cov_4.522134, whole genome shotgun sequence.
ACCESSION   DAOSRB010000265 DAOSRB010000000
VERSION     DAOSRB010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24425_length_3466_cov_4.522134"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /gene="cas3u"
                     /locus_tag="PLZ74_10545"
     CDS             complement(<1..1466)
                     /gene="cas3u"
                     /locus_tag="PLZ74_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated helicase/endonuclease
                     Cas3"
                     /protein_id="HOR98797.1"
                     /translation="MTQTADTFFAQQYALLRHPYKPMKWMSRMFNDLIKGEVPSVVDL
                     PTASGKTDVIVIWIIALAWYAQNRGKTNPIPRRLVWVVNRRVLVQQVDDMAQKLIAIL
                     HGKSNNSPELELAKLLRGLCANQDKSAPCFNVVQLRGQRLDDREWTLDPTIPQLIIGT
                     VDQIGSRLLFQGYGLGKWSRPLHAGLLGVDAWVCVDEAHLVPSFVTTLRQVREIASRP
                     IAHDIPQAIKDVFSQLPFWDSELSATPGLPKPKAGMTFALQTEDHADPVIKDRLKARQ
                     KRRVIQEPLPNNKELASALAKKALGLDVIKNSGTVAVFCSKVKDAEEVAQELHKKHRD
                     RVLLVTGRVRGYERDRLVKGELFKRFRGEKAENADCQPAFLVGTAAAEVGLDADADAI
                     VCDFAPLPTLIQRLGRLDRTGRISKNLKHNAPDDARPTMHIIGGEKGKISEAELKKLA
                     EALSAPSNDKSEFDPSFFSGSAWVPRKTKDAAGQSDTD"
     gene            complement(1471..1596)
                     /locus_tag="PLZ74_10550"
     CDS             complement(1471..1596)
                     /locus_tag="PLZ74_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98798.1"
                     /translation="MNDFASMFEKATGHKRPHDYQSRLALSSGEKQLKDQHLFRW"
     gene            1780..>3466
                     /locus_tag="PLZ74_10555"
     CDS             1780..>3466
                     /locus_tag="PLZ74_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98799.1"
                     /translation="MKRKNTGMARKVRMAVMTVTFIAGVRFVAEAQTVWVSAEDGVWS
                     DAAAWTAGVPDANAAFLTNQTASYKVTVEEPPATHYGNLTLANAAGNTTRLDVAASGF
                     ASTNGMLSIGRGATVAVNTGGVMGYTGRTPATAPFVEIKDGGVWRVDGGTIDFTDLRR
                     VTPTSGSCYVYVGNGSTGRMEVTSGEVLFKGVESSGETNKTVVVRVGNGTGGCGELVM
                     SGGSMTLYNPSSEWSQRVLDIGANGIGVMGAVSLSGEALMLVSNMVYVGGQQGTGTLD
                     VAGNAVFRMPRSNRLYVGKLSKALGIVTVRENGFLNLGGTDGLQMGTDSGRGILNIEG
                     GRAEVCGLHLSVSGSPWAGGYGELNLTGGMLTHRTGNGYGISVARAHTATAGAYGVIR
                     MTGGLIDHSLGLWQQDWSQNGIILGKVGNAANVGGNALGEFFISGGTVTNSGQFILGM
                     GPGATGVVVQTGGDIRQAVVNPSPRSFLTVGWGGGCGSYTLSGGSVVSWRNTYIGGHF
                     TNDYGYVPTDVAFTSNSVGVLRIDGGYLTVTNADLFIGRNGTGTLILGSNGVCT"
BASE COUNT          717 a          867 c         1078 g          804 t
ORIGIN      
        1 tcatcggtat cggattgacc ggcagcgtct tttgtttttc ggggcaccca tgccgaaccg
       61 gagaaaaaag aaggatcaaa ttcgctcttg tcattgcttg gagcggaaag ggcctccgcc
      121 aatttcttga gttccgcttc acttatcttt cctttttcgc cgccgattat gtgcattgta
      181 ggacgcgcat cgtccggcgc attatgtttt aggtttttac tgattcgtcc tgtacgatca
      241 agacgtccta gccgctgaat aagcgtcgga agtggcgcga agtcgcagac aattgcatcg
      301 gcatcggcat ccagaccgac ttcagccgct gccgtgccga caaggaaggc gggctgacag
      361 tctgcgtttt cggctttttc accgcggaag cgtttgaaaa gctcaccctt taccaaccgg
      421 tcgcgttcat aaccacgtac acggcctgtc accagcagga cgcgatccct atgcttcttg
      481 tggagttctt gagcaacttc ctccgcatcc ttgacttttg aacaaaacac cgcgacggtg
      541 ccgctgttct tgataacatc aagtcccagt gcttttttcg ccaaggcaga ggctaactcc
      601 ttattgttgg gaagcggctc ctgtatcaca cgccgctttt gtctggcttt gaggcgatcc
      661 ttaatgaccg gatcagcatg gtcttctgtt tgcaaagcga atgtcatacc cgctttaggc
      721 ttaggcaacc ccggtgtcgc tgaaagctct gagtcccaaa atggcaattg gctgaaaaca
      781 tctttgatgg cttgcgggat atcgtgtgct atcgggcgtg aagcgatttc acggacttgt
      841 cggagcgtgg tgacaaatga cggcacaaga tgcgcttcgt ctacgcagac ccaagcatcc
      901 acaccaagaa gtccggcatg aagcggacgt gaccacttgc caaggccgta tccttgaaac
      961 aagaggcgtg agccgatctg gtcaaccgtg ccgataataa gttgcgggat tgtcggatca
     1021 agtgtccatt cacgatcatc aagacgttgt ccacgcaact gaacaacatt gaagcacggg
     1081 gctgatttgt cttggtttgc gcataggccg cgcaggagtt tagcgagttc gagttcaggg
     1141 gagttgttgg atttgccatg aagaatcgca atcagttttt gcgccatgtc atctacctgt
     1201 tggacaagga cgcgacgatt gaccacccat acgagtcggc gcggaatggg attggtttta
     1261 cccctgtttt gggcatacca cgcaagtgcg atgatccaaa taacgatcac atctgttttg
     1321 cctgatgcag tcggcaagtc aaccaccgag gggacttctc ctttgatcag atcgttgaac
     1381 atccggctca tccacttcat cggtttatac ggatggcgga gtagagcata ctgttgtgcg
     1441 aaaaaagtgt cggctgtttg tgtcatactg tcaccaacgg aacaagtgtt gatctttcag
     1501 ttgtttttcc ccgctactca aagcaagacg gctctgataa tcgtgcggcc ttttatgtcc
     1561 ggtcgctttt tcaaacatgc tggcgaaatc attcatgggg ctctccgatt tcaacaggat
     1621 gtctgcacaa tacaccctat gcatttaact ggcaagcgct tttccgctga tttggattgc
     1681 gttttttcaa tccgttcgca tacaatatgc aaggagcttt tgaggggttt cttcctgacg
     1741 ggaagaatgg ttgtgttcaa gcctaaaaag gagtcgcaga tgaaacggaa aaatacgggg
     1801 atggcacgaa aggtccggat ggctgtgatg acggtcacgt ttatcgcggg ggtgcggttc
     1861 gttgcagagg cgcagaccgt atgggtgagt gcggaggacg gggtgtggtc ggatgcggct
     1921 gcgtggacag ccggtgttcc ggatgccaac gcggcgttcc tgaccaatca aaccgcctcg
     1981 tacaaggtca cggtggaaga gccgcccgca acgcattacg gcaacctgac cctcgccaat
     2041 gcggcgggca acacgacgcg gctggacgtg gccgcgtcgg ggtttgccag caccaacggg
     2101 atgctgtcga tcggtcgcgg ggcaacggtc gccgtgaaca ccggaggtgt gatggggtat
     2161 acgggacgca cgccggcgac cgcacccttt gtcgaaatca aagacggcgg cgtgtggcgt
     2221 gtggacggcg ggaccataga tttcacggat ttgcggcggg tcacgccgac cagcggtagt
     2281 tgttacgtct atgtcggtaa cggctcgacc gggcgtatgg aggtcacgtc cggagaggtc
     2341 ctgttcaaag gggtcgaatc gtctggcgag accaacaaaa cagtcgttgt gcgggtgggg
     2401 aatggaaccg gcggatgcgg cgaactggtc atgtccggcg ggagcatgac tctgtataat
     2461 ccctcttccg aatggagtca gcgtgttctc gatatcgggg ccaacgggat tggggtgatg
     2521 ggcgcggtct ccctgtccgg ggaggcgctg atgctggtca gcaacatggt gtatgtcggg
     2581 ggccagcagg gaaccggcac gttggatgtc gcagggaacg cggtgttccg gatgccgcgg
     2641 agcaatcgcc tttatgtggg caagttgtcc aaggcgttag ggatcgtgac ggtcagggag
     2701 aacgggtttc tcaatctggg tggaaccgat gggttgcaaa tgggaacgga ctcgggacgc
     2761 gggattctca atatcgaggg cggacgggcg gaggtgtgcg gattgcatct gagcgtttcg
     2821 ggatcgcctt gggcgggtgg atacggtgag ctcaatctga cgggcggcat gctcacgcat
     2881 cggaccggaa acggatacgg aatctcggtc gcccgggctc ataccgccac agcgggcgcg
     2941 tacggtgtga tacggatgac gggaggcctg attgatcact ccctcgggtt gtggcagcag
     3001 gactggtcgc agaacggtat cattctcgga aaggtcggaa acgccgcgaa tgtgggcggt
     3061 aacgcgttgg gcgagttttt catcagcggc ggaacggtga caaactcagg ccagttcatt
     3121 ttgggtatgg ggcccggcgc aacgggtgtg gtggtccaga ccggaggaga tatccgacag
     3181 gcggttgtga atccttcgcc tcgatctttc ctgaccgtcg ggtggggtgg gggatgtggc
     3241 tcgtacacgc tttcgggcgg atccgtcgta tcttggcgca acacctatat cggggggcac
     3301 ttcacgaacg actatggcta tgtgccgacc gatgtcgcgt tcaccagcaa cagcgttggg
     3361 gtgttgcgca tcgacggcgg ctacctcacg gtcaccaatg ccgatctctt tatcgggcgg
     3421 aacggcacgg gcacgctgat cctcggttcg aacggcgtct gcacgg
//
LOCUS       DAOSRB010000266         3389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_25146_length_3389_cov_4.878224, whole genome shotgun sequence.
ACCESSION   DAOSRB010000266 DAOSRB010000000
VERSION     DAOSRB010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25146_length_3389_cov_4.878224"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..3178)
                     /locus_tag="PLZ74_10560"
     CDS             complement(278..3178)
                     /locus_tag="PLZ74_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98800.1"
                     /translation="MSSAQESVTLPIERRMLFNEWVFWHAGSYYHPNWTHEGPAPANF
                     PPARRMLLPGTNHYHAAGWRLTACRTGTSAGPTGQGVNFIPPLSYNPTIQTNWQANLQ
                     NTLDAKIESPFYPEGIGTLYFEAINVFSSYPTEITVEIATNMLEYLYLGGGVTNVMYP
                     VESDQFSYNWQVLDVLPLAAASSNDFTRYQRLLQCRTPARFRIRRTGGVYSTQPLDNA
                     LTAIDNICASYPPADVVLVRPAAVFKPGYPAVGEPCVIRCEVSCVGTNWNERTDHTSR
                     AMTVHYRWRYLDQMSNAWASVPMSYVAGSGVGDGEVYEATLPAQADVGDLEYFFECEF
                     EGYRYWHLDYTGSGYAYTTEWLSPRILRGDTAVEGGREFYTRLRPMASSFGEMLLVVG
                     EPGAEQATVVMTPLETGRWRGIIPLAGRAPTNICFYLRGEGQYDAVADAFLPDPVYWS
                     DASQTVPSPLPLTGTCDSGAVPPNHRIRIATAGSSCVEVLFDEVSGTYWTARAEYQNF
                     NSQTADTDPIQDHDQPFLQSPALSFPLGSLTFMARAYDVASPARIAVCASTNGWDAPA
                     ESWFEVHAFSGIAHVPYRTYRFVPATPVQYDAIRLVVAADGSSGRACVDEVVISELGA
                     AGVPPSVKVFVPTEPVSELAGTGAYTKKRLIVRLSEPYAQDVTVRLDLSPDANAQNGC
                     LGLSQTNLVFAAGAEEQVVDIGPLDGTDLSATDGFTVTPVVVSPVAAQHHYLSLVAVA
                     VRVLNEAPVIVEPAGGIASLPASFFVQCGQPHSFFWNVTDAAADSGADVGSGMKITWY
                     FGDDTWSAAWGASGMIEHTYANVGDRVVRMIAEDKDGGFDEVQFRVRVATEPVTLSTP
                     VPVPLAWLDLYPSALAAAGGNYESAALGDVDGDGHTTWQEYVTGSDPTNTLSILRAHI
                     AVDGGTAQVTWTPDLGPVARCYTVEGKTNLTDGVWLESGPGMRFFRVRVALP"
BASE COUNT          740 a         1093 c          904 g          652 t
ORIGIN      
        1 gcatgactcg ccacagtaat ggaaaaaagt gtcagcacct ttgtttttaa agacacagcg
       61 aatatccttt cttctcttca atcacacccg ccgagcattt cgcctgggag gttaccggcg
      121 tcgcagtttg acgttagaca tttcgagttt tcagccacgc cgcccatcct ttcggtggta
      181 cctcattcgc cttgccacgg cgacacctta ccgcaccgcg aaaacgcttc gcaacccttg
      241 attgtctcat gtcaaaacac gcccccgttt ctcaccttta cggcaacgcc accctgaccc
      301 tgaagaagcg catgccgggt ccactttcca gccaaacccc atccgtcagg ttcgtcttgc
      361 cttcaaccgt gtagcaccgg gcgaccgggc cgagatccgg cgtccatgtg acctgcgccg
      421 tcccgccgtc aacggcgatg tgggcgcgga ggatggagag cgtgttcgtc ggatccgaac
      481 cggttacgta ctcttgccaa gtcgtatggc cgtcgccgtc cacgtcgccc agcgccgccg
      541 actcgtaatt gccgccagcc gctgccaacg ccgacggata cagatccagc catgcgaggg
      601 gtaccggcac cggggtcgac agtgtcacgg gttctgtcgc gactctgact ctaaactgaa
      661 cttcatcaaa accgccgtcc ttgtcttctg ctatcattct gaccacccgg tcacccacgt
      721 tggcatacgt atgctcgatc atgccgcttg ccccccaagc cgccgaccac gtatcatcgc
      781 cgaagtacca tgtgatcttc attccgcttc ccacatcggc accgctgtcc gctgcggcat
      841 ccgttacgtt ccagaagaag gaatgcggct gcccgcactg gacgaagaac gacgcgggca
      901 gcgatgcgat gccaccggct ggctcaacaa tcacaggggc ctcgtttagg acccgcaccg
      961 caaccgcgac caaagacaag tagtggtgct gcgcggcaac cggcgacaca accactggcg
     1021 tgaccgtaaa gccatccgtt gccgaaagat ccgtgccgtc cagggggcca atgtcgacaa
     1081 cctgttcttc cgcaccggcc gcgaagacca agttggtttg cgacaagcca aggcaaccat
     1141 tttgcgcatt ggcatccggc gaaagatcca agcgcaccgt cacatcctgc gcatagggct
     1201 ccgagagtct gacaatcaag cgtttctttg tatatgcccc tgtccccgcg agttccgaaa
     1261 ccggctccgt ggggacgaac accttcacgc taggcggcac ccccgcagcc cccagttcgc
     1321 tgatcaccac ctcatccacg caagcccgcc ccgacgatcc atccgccgca accaccaagc
     1381 ggatcgcgtc atattgcacc ggcgtcgccg gtacaaatcg gtaggttcgg tatggaacgt
     1441 gcgcaatgcc ggagaacgcg tgtacctcaa accaactctc ggcaggcgca tcccagccgt
     1501 tggtcgacgc acacaccgcg atccgcgccg gagacgccac gtcgtaggcg cgggccatga
     1561 atgtgagcga ccccaacggg aaggagagcg ctggcgattg caggaaaggc tgatcatgat
     1621 cttgaatcgg atccgtatcc gccgtctggc tgttgaaatt ctggtactcc gcgcgcgcgg
     1681 tccaataggt gccggatact tcatcaaaca agacctccac acaactgctt ccggccgttg
     1741 cgatgcggat gcggtgattc ggcggaacgg caccgctgtc acacgtgcct gtcagcggca
     1801 agggagaagg taccgtctgt gatgcgtcgc tccaatacac cggatcgggc aggaaggcat
     1861 cggcgaccgc gtcgtactgg ccttcgccgc gcaggtagaa gcagatgttg gtaggagccc
     1921 ggcccgcaag cgggatgata ccgcgccagc gtccggtttc aagcggcgtc ataaccaccg
     1981 tcgcctgttc ggcacccggc tcgccgacca ccagcagcat ctcgccaaag gacgaggcca
     2041 tgggccgcaa ccgcgtgtaa aactcgcgcc cgccctccac cgccgtgtcg ccgcgcaaaa
     2101 tacggggcga cagccactcc gtcgtgtacg catagcccga gcccgtataa tcaaggtgcc
     2161 aataacgata cccttcaaat tcacattcaa agaaatactc cagatcgccg acatcggcct
     2221 gcgcgggcaa ggtcgcctca tacacctcgc catctccgac gcccgacccc gcaacatagc
     2281 tcatcggtac actggcccat gcgttggaca tctggtccag ataccgccat ctatagtgaa
     2341 ccgtcatcgc tcggctcgta tgatcggtcc gttcattcca gtttgtcccc acgcaactaa
     2401 cttcgcaccg gatcacacac ggctcgccaa cggcgggata ccccggcttg aagaccgcgg
     2461 ccggacgaac caacacgaca tccgccgggg gatatgaggc gcagatgttg tcgatcgcgg
     2521 taagcgcatt gtctagcggt tgtgtagagt atacgcctcc cgttctacga atacgaaatc
     2581 tggccggcgt acgacactgc aacaaccgtt gatagcgcgt aaaatcgtta gatgacgccg
     2641 ccgcaagggg caacacgtca aggacttgcc aattgtatga aaactgatct gactcgaccg
     2701 gatacatgac atttgtgacg ccgcccccca gatacagata ctccagcata ttggtggcga
     2761 tctcaacggt gatttcagtc gggtaggatg agaacacgtt gatggcctca aaatagaggg
     2821 tgccgatacc ctccgggtag aacgggcttt cgatttttgc atccaaggtg ttttgcaaat
     2881 tcgcctgcca gtttgtctgg atcgtggggt tgtaagagag cggcggaatg aaattaacgc
     2941 cctgccccgt cggccccgcc gatgtgcctg tacggcacgc cgtcaaccgc catcccgccg
     3001 catgatagtg attcgttccg ggcagcaaca tacggcgcgc cggcgggaaa ttggcgggag
     3061 caggtccctc atgcgtccag ttcgggtgat agtacgaacc ggcatgccaa aacacccatt
     3121 cgttaaacaa catgcgccgt tcgatgggaa gcgtcaccga ttcctgagcg ctactcatgc
     3181 ttacgactgc aaaaagaatg atacccaccc ctaccaacgg attacgcata cgcacctcca
     3241 gcacatctcg gtttcggtgt taacgtcgtc acctttcagc gcaagtctct ccccacaaca
     3301 agcttggtgt aatggtagac cagcgacaga tgggaagtca accggtttta cgtggaaagg
     3361 gagtgtccga ttggtgtata ctcgcccgc
//
LOCUS       DAOSRB010000267         3386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_25174_length_3386_cov_6.039928, whole genome shotgun sequence.
ACCESSION   DAOSRB010000267 DAOSRB010000000
VERSION     DAOSRB010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25174_length_3386_cov_6.039928"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..3032
                     /gene="uvrA"
                     /locus_tag="PLZ74_10565"
     CDS             204..3032
                     /gene="uvrA"
                     /locus_tag="PLZ74_10565"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOR98801.1"
                     /translation="MTDILISGARQHNLKNVDVRIPRDSLTVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLDRMQKPDVDHIEGLSPAIAIEQRTSGGNPRSVVATVT
                     EIHDYLRLLYGSIGQAHCPECGKPVGRQSAEQIVDRLSGEPAGSKLILLAPLVRGRKG
                     RHEEVLEAARRQGFVRIRVDGNLFALDDVPRLEKNKAHTLEVVVDRLVAGDGVHARLT
                     DSVELGLKQGNGLIQVQVTRPDGKTREEVFSEKNACPDCGISFEELKPRSFSFNNPYG
                     ACPVCAGLGAQLVFDEERVVPNKTLSLAEGAIQPWRHGGFRMVVYYKHLTKAVAAAYG
                     VDLNTPYQELPERFRQILLHGSGEEPVTLRFWMRGAWRKTEKPFEGVLPSLLRRYNES
                     EFDEVREKLRQYMSRQTCPACQGARLRPEARVCTVSGRSLVEVMAMTIRQGVAFFDKL
                     KLDAAQVAAVSEVLKEIRRRLAFLSDVGLDYLSLDRESSTLSGGEMQRIRLATQIGSG
                     LVGVLYVLDEPTIGLHPRDNERLIGMLRRLQRRGNTVVVVEHDEEMIRNADHIVDLGP
                     RAGRQGGEVVYQGGLKGLLASRHSLTADYLTGRMRIAIPEARVQPGPARLTIKGAAEN
                     NLRNIDVEIPLGCFVCVTGVSGSGKSTLVGEILKNYLFRHFYGSKERVGTCKKITGIE
                     GIDSVIEIDQSPIGRTPRSNPVTYTGAFTPIRSLFAATPAAKVRGYGPGRFSFNVKGG
                     RCEVCKGDGVRCLEMHFLPDVYVTCEQCGGQRYNKETLEVRYGGRNISEVLNLTVDEA
                     LEFFAPVPAVAKKLRTLSEVGLGYIHLGQASTTLSGGEAQRIKLAAELSKRSTGRTLY
                     LLDEPTTGLHFADIHKLLDLLLRLRESGNTVVVIEHNPDVIKCADHLIDLGPGGGDEG
                     GWLVATGTPEEVAACPDSVTGRFLKRHL"
BASE COUNT          593 a          905 c         1206 g          682 t
ORIGIN      
        1 ataggggcac gggttttcca aataccggga aggtgctcat accatgaccg cgaaacgctg
       61 gcttggatcg cgtgcggaaa agagcggttg acgcctttgg ggaggctccg tacacttcct
      121 tccctatcag gggatcaggc ggcggacgcg gacggtctcc ggaggcctgg ccgggggcgg
      181 acttgtccgg cgcggtgatc tttatgacag acatcctcat cagtggcgcg cggcagcata
      241 atctcaagaa tgtcgatgtg cgcatcccgc gtgattcgct tacggtgatc accgggctca
      301 gcggttccgg caaatcgtcg ttggcgtttg acacgctcta tgccgagggg cagcgccggt
      361 atgtcgagag tctctcggcc tatgcgcggc agtttctgga ccggatgcag aagcccgacg
      421 tggaccatat cgaagggctg tcgcccgcga tcgcaatcga gcagcggaca tccggcggga
      481 atccccgctc cgtcgtggcc acggtgacgg agatccacga ttatctgcgc ctgctgtacg
      541 gcagcatcgg acaggcgcat tgtcccgagt gcggcaaacc ggtagggcgg cagagcgcgg
      601 agcagatcgt ggaccgcctg tcgggcgagc cagcggggag caagctgatt ctgctggcac
      661 ctttggtgcg gggacgcaag ggtcggcatg aggaggtgct cgaggcggcc cggcgtcagg
      721 gttttgtccg gatccgtgtg gatgggaacc tgtttgcgtt ggacgacgtg ccgcggctgg
      781 agaagaacaa agcgcacacc cttgaggtgg tggtggaccg gctggtggcg ggggacgggg
      841 tgcatgcgcg gctcaccgac tcggtcgagt tggggctgaa acagggtaac ggcctcattc
      901 aggtgcaggt gacccggccg gacggtaaga cgcgcgagga ggtgttttcc gaaaagaatg
      961 cgtgtccgga ttgcgggatc agttttgaag agctaaagcc gcggtcgttc tccttcaaca
     1021 atccgtatgg cgcctgtccg gtttgtgcgg gattgggcgc ccaattggtt tttgacgagg
     1081 agcgggtggt gccgaacaaa accctgtcgc tggccgaagg cgcgattcag ccctggcgtc
     1141 acggcgggtt ccgcatggtg gtttactaca aacacctcac caaggcggtg gctgcggcgt
     1201 acggggtgga tttgaacacg ccgtatcagg agttgcccga gcgtttccgg cagattctcc
     1261 tgcacggtag cggggaggag ccggtgacgc tgcggttctg gatgcgcggt gcctggcgca
     1321 aaaccgaaaa gccgttcgaa ggcgtgctgc cgagtctgtt gcggcgctac aacgaatcgg
     1381 agtttgacga ggtgcgggaa aaactccggc aatacatgag ccgtcagacc tgtccggcct
     1441 gtcaaggggc gcgtctgcgt cccgaggccc gcgtctgcac cgtgtcaggc cgttcgctgg
     1501 tggaggtgat ggccatgacg atccggcagg gggtggcgtt tttcgacaaa ctgaagttgg
     1561 atgcggcgca ggtggcggcg gtctccgagg tgttgaaaga gattcgccgc cggctcgcgt
     1621 tcctttcgga tgtggggctc gattatctct cgctcgaccg ggagagttcg accctttccg
     1681 gcggcgagat gcagcgcatt cggctggcca cgcagatcgg gtccgggctg gtcggtgtgt
     1741 tgtatgtgct ggacgagccg acgatcggtc ttcatccgcg tgacaacgag cggctcatcg
     1801 gcatgctgcg ccggctgcag cggcgcggca acaccgtggt ggtggtcgag catgacgagg
     1861 agatgatccg aaatgcggac cacattgtgg atttggggcc ccgggccgga cgtcagggtg
     1921 gcgaggtggt gtatcaggga gggttaaagg gactgctggc cagcaggcat tccctgacgg
     1981 ccgattacct gaccggacgc atgcggatcg cgatccctga ggcgcgcgtc caaccgggtc
     2041 cggcccggct caccatcaag ggggctgcgg aaaacaatct ccggaacatc gatgtggaga
     2101 tcccgctcgg ttgtttcgtc tgtgtgacgg gggtctccgg gtccggcaaa tccacactgg
     2161 tgggcgagat actcaagaac tacctgttcc gccatttcta cggttccaaa gagcgcgtgg
     2221 ggacctgcaa gaaaatcacc gggatcgaag ggatcgacag cgtcatcgag attgatcaga
     2281 gcccgatcgg gcgcacgccc cgcagcaacc cagtcaccta caccggggcc ttcacaccga
     2341 tccgcagcct gttcgccgcc acgcccgcgg ccaaggtgcg cgggtacggt ccggggcgct
     2401 tcagcttcaa cgtcaagggc ggtcggtgcg aagtgtgcaa gggggacggc gtgcgctgtc
     2461 tggaaatgca ctttctgccg gatgtgtacg tgacctgcga gcaatgcggc gggcagcgtt
     2521 acaataaaga gacactggag gtccggtacg gtggcaggaa catctccgag gtgttgaatc
     2581 tgacggtgga cgaggcgctc gagttctttg ccccggtgcc ggcggtcgcc aagaaactgc
     2641 ggactctatc cgaggtcggg ttgggataca ttcatttggg tcaggcctcg acgacccttt
     2701 cggggggcga ggcgcagcgc atcaagctgg ccgccgaact gtcgaagcgc tcgaccggcc
     2761 ggacgctgta tctgttggac gagccgacga ccggcctgca cttcgcggat attcacaaac
     2821 tgctcgatct cctgttgcgg ttgcgtgaat ccggcaacac ggtggtggtg atcgaacata
     2881 atccggatgt gatcaaatgt gcggatcacc tcattgatct cgggccgggc gggggggacg
     2941 agggcgggtg gctggttgcg accggcacgc cggaggaggt cgccgcctgc ccggactccg
     3001 tgacgggacg ctttctgaag cggcatctgt gaggcgcgct gacgcttttt ggggggaggg
     3061 aacgtgggat gtgtccgtca tgcctgtcgc gaagcatggg cgggggacgg ttccctgaac
     3121 cgtccgcgga cgcttcgggg aggcgtccct accgtgaacc taaaaagagc agttgaacct
     3181 tcaactctct tgggtttctg ggtttcaagg tttctgggtt tcagggtttc atcggggtcg
     3241 ggatcgggat cgctatcggg tttctgggtt tcagggtttc atcggggtcg gggtcgctat
     3301 cgctatcggg tttcagggtt tctggtttca gggtttcagg gtttctggtt tcaaggtttc
     3361 tggtttcagg gtttcatcca aatcgg
//
LOCUS       DAOSRB010000268         3345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_25642_length_3345_cov_6.558663, whole genome shotgun sequence.
ACCESSION   DAOSRB010000268 DAOSRB010000000
VERSION     DAOSRB010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25642_length_3345_cov_6.558663"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLZ74_10570"
     CDS             complement(<1..369)
                     /locus_tag="PLZ74_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98802.1"
                     /translation="MGLEVRYGRGGELRGHWYAALVDSNGKRRVVSLTEPLPTRHFPG
                     SLRETGDAVFEASRARAQKELEGFQTDARVKGRSQHLTERLIQSKTGRAVEYVKLADL
                     PAKWRGLERETTPGDNWLAWC"
     gene            477..566
                     /locus_tag="PLZ74_10575"
     tRNA            477..566
                     /locus_tag="PLZ74_10575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:550..552,aa:Leu,seq:caa)
     gene            complement(635..>3345)
                     /locus_tag="PLZ74_10580"
     CDS             complement(635..>3345)
                     /locus_tag="PLZ74_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOR98803.1"
                     /translation="LPRNNYFVAEGKPADVEKPVPNRLIAEAGDLDTCRDDLTNGVEF
                     VAMHGWNITRARVKAFDRVTREVTLAVGRPGYLRGFDSNSYYRLENVKSAYGEVEGEW
                     YCEPQTGLLSYLPLPNETPVNAPFVVPVLTNLVTVQGDRATGRKATNVSFQSLGFAYV
                     SEPLQEPGTVYHQTSLEAGTAIRVDFAENVSIDACAVRHTGGWAVKIEGGCVGCAVTG
                     STFFDLGAGGVALGVKGARRGNEERHTHDCRVERCLIEGGGRVQAGAVGICVVLAGGN
                     RIAHNTIRDFYYSGISVGYDWSDKNDAASGNIVEWNHIYDIGQRVLSDLGGIYTLGRQ
                     PGTILRCNLIHDIRTARYGAQGIYMDQGSAEILACSNIITRADEAAFSVASGACGGIR
                     FENNIFLFPRRYVIWTFNDKQKGHDVTVARNIFMWDDKLRDMFPEGGPAKEWRFSSNV
                     WWQAAWPERIYFRKPGGTNAFVLADAPAGSRWKQWHKLAMPSPDVAAWLAAAPGDTLE
                     DPRLGAAYGDEKHLKPWPDSPAVVRGFVPFDLSAGVGHEPQAQPYARKRPLVSATRTF
                     PAAPLSRKNKAALAAETAKGELHMTVFNQRYILGGAGMGVAFRTPAGKTFFFDMGNGD
                     DTVVAHNCGRDTIDPWLYVNDIRKIDGVVMSHAHGDHFGGLLALWNHYPVDYFWDNDY
                     RMPGQPVNAQNFGELGAVRRLRAQLKSVNPHLVCRGVRQFDKLDWDPVLDVTVITPPK
                     AGIPYLVNPNRPKNDGPEHHLINANALGIRVEYGKVSYQIVGDIQADYEKAFLLPTLP
                     DKFKTCDILMPCSHGIHATDEAANVFRPKVAIGTVYADGAADGWSRTWAFTLPIWNVY
                     RKVGAEVYVSGVDGDVTASSDGKTWRVTTEFGANRRVNPFSRPARR"
BASE COUNT          635 a         1054 c          991 g          665 t
ORIGIN      
        1 acaccaagcc agccagttat cgcccggcgt ggtttcccgt tcaagaccgc gccactttgc
       61 cggaaggtcg gcaagcttca cgtattccac cgcccgcccg gtcttagact ggataagccg
      121 ctcggttaaa tgttgactcc gcccctttac cctcgcatcg gtctggaagc cttcaagctc
      181 cttttgcgcc cgcgcccgtg aagcctcaaa aacggcgtcg ccggtttccc gcagactgcc
      241 ggggaaatgc cgggtcggta gcggctcggt cagcgagaca acccgccgct tgccgttgct
      301 gtccacaagt gcggcgtacc agtgccctcg gagttcgccg ccccgcccgt acctgacttc
      361 cagacccata gccgccccct tttccggtgt tttgaaacaa cgggaacgat tttgaccgaa
      421 cgggggcaaa agagcaagcg aaaaacacac acaacaacaa acacatgttt tttgaagggt
      481 caaaaggtgc ggttttatta tggtttatca gttttcggta tttggttcgg gaccaaaagg
      541 tcgagggttc aaatccctcc gctccgacca atttttcaga gtgcaaatcc acgaaaacgc
      601 tcatgttttc gcggtttttt gtttggggaa gtcatcaacg ccgtgccggg cgggagaagg
      661 gattcacgcg gcggttcgct ccgaactcag tcgtcacgcg ccatgttttc ccgtcagaag
      721 acgccgtcac gtcgccgtcg actcccgaga cgtagacctc tgcgcccact tttctgtaga
      781 cgttccagat gggcagggtg aacgcccatg tgcggctcca cccgtccgcg gcaccgtcgg
      841 cgtacaccgt gccgatggcc actttcgggc ggaacacgtt cgccgcctcg tccgtcgcgt
      901 ggatgccgtg tgaacacggc ataaggatgt cacacgtctt gaacttatct ggcagcgtgg
      961 gcagaaggaa tgccttttca tagtcagcct gaatgtcgcc gacgatctgg taggagacct
     1021 tgccgtactc cacgcggata cccagcgcgt tcgcattgat aagatggtgc tcgggcccgt
     1081 cgttcttagg gcggttcgga ttgaccaagt acggaatgcc ggccttcggg ggcgtgatca
     1141 cggtcacgtc cagcacagga tcccaatcca gtttgtcgaa ttggcgcaca ccgcggcaga
     1201 caagatgggg gttgacggac ttgagttgtg cgcgcaaacg ccgtacggca ccgagttcgc
     1261 cgaaattctg cgcgttcacg ggctgtccgg gcatgcggta gtcgttgtcc cagaaataat
     1321 ccacgggata gtggttccac aaggcaagca gcccgccgaa gtggtcgccg tgggcatgac
     1381 tcatcaccac gccgtcgatt ttacggatgt cgttcacgta gagccaggga tcgatcgtgt
     1441 cgcggccgca gttgtgagcc accacggtgt catcgccgtt acccatgtcg aagaagaacg
     1501 ttttgccggc aggtgtgcgg aacgccacgc ccatgccagc gccgccgagg atatagcgct
     1561 ggttgaacac cgtcatgtgc agttcgccct tggccgtttc ggcggcaaga gccgccttgt
     1621 ttttgcgcga gagcggcgcg gcggggaacg tgcgcgtggc cgagaccaag ggacgtttcc
     1681 gagcgtaggg ctgcgcctgt ggctcgtgcc cgacacctgc cgaaagatcg aatggcacga
     1741 agccgcgcac gacggcaggg gaatccggcc agggcttcaa gtgcttttca tcgccatagg
     1801 cggcaccgag gcgcgggtct tccagcgtgt cgcccggagc ggcggcgagc cacgccgcga
     1861 catccggact gggcatggcg agtttgtgcc actgtttcca gcgcgaacca gcgggcgcgt
     1921 cggcgaggac aaacgcgttc gtgccgccgg gtttacgaaa ataaatgcgc tcgggccaag
     1981 ccgcctgcca ccacacgttg ctgctgaaac gccactcttt ggcaggacct ccctccggga
     2041 acatgtcccg cagtttgtca tcccacatga aaatgtttcg tgccactgtc acgtcgtgcc
     2101 ccttctgctt gtcattgaat gtccaaatga catagcggcg cggaaagagg aagatgttgt
     2161 tctcgaatcg gatgccgccg caagcgccag acgccacgga gaacgccgct tcgtccgcac
     2221 gcgtaatgat gttgctgcat gcgaggattt ccgcgctgcc ttgatccatg tagataccct
     2281 gcgcgccata gcgggccgta cgaatgtcat gaataaggtt acaacgcagg atcgtgcccg
     2341 gctggcgtcc gagcgtgtag atgccgccga ggtcagataa gacacgctgg ccgatgtcgt
     2401 agatgtggtt ccactcaacg atgttgccgg acgccgcgtc gttcttgtcg ctccagtcat
     2461 atcccacgct gatgccgctg tagtagaaat cgcgaatcgt gttgtgcgcg atccggtttc
     2521 cgcctgcgag taccacgcag atccccacgg caccggcctg gacgcgtccg ccgccctcta
     2581 tcagacaccg ctccacgcgg caatcgtgcg tatggcgctc ctcgttgcca cgccgcgcgc
     2641 ccttcacgcc cagcgccacg ccccccgcgc cgaggtcgaa gaacgttgac cccgtgaccg
     2701 cgcaccccac gcagccgcct tcgatcttca cggcccagcc gcccgtgtgg cgcacggcgc
     2761 aagcgtcgat cgacacgttt tccgcaaaat cgacccggat cgccgttccc gcctcaagcg
     2821 atgtctggtg atacaccgtg ccgggctcct gcaagggttc ggagacgtag gcgaaaccca
     2881 acgactggaa cgacacgttc gttgccttgc gtcccgtcgc gcggtcaccc tgcacggtca
     2941 cgagattggt tagaacgggc acgacgaacg gggcgttcac gggcgtttca ttcggcaacg
     3001 gcagatagga gaggagtccc gtctgcggct cgcaatacca ttcgccttcc acctcgccat
     3061 acgcgctctt cacgttttca agacggtagt aggaattgga atcaaagccg cgcagatagc
     3121 ctggccgtcc caccgcgagc gtcacctcgc gcgtgacgcg gtcgaacgcc ttcacgcgcg
     3181 cccgcgtgat gttccagcca tgcatcgcca cgaactcgac gccattcgtg agatcgtccc
     3241 ggcacgtatc caaatcgccc gcttccgcga tgagacggtt cgggacaggc ttctcgacat
     3301 cggcgggttt cccttctgcg acgaagtagt tgttgcgcgg caggg
//
LOCUS       DAOSRB010000269         3342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_25685_length_3342_cov_5.919988, whole genome shotgun sequence.
ACCESSION   DAOSRB010000269 DAOSRB010000000
VERSION     DAOSRB010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25685_length_3342_cov_5.919988"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLZ74_10585"
     CDS             <1..266
                     /locus_tag="PLZ74_10585"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOR98804.1"
                     /translation="LGLIAFSFGTAGGCWMGRLMNKFCKEPINPLIGSAGVSAVPMAA
                     RVSNKVGLESDPNNYLLMHAMGPNVSGVIGSAVVAGVLYTLCK"
     gene            complement(281..1321)
                     /gene="mutY"
                     /locus_tag="PLZ74_10590"
     CDS             complement(281..1321)
                     /gene="mutY"
                     /locus_tag="PLZ74_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOR98805.1"
                     /translation="MPPSLTERLLSWYRINRREMPWRDHPDPYAVWVSEIMLQQTRVD
                     TVRGYFVRFLEAFPTVRTLAEAPEQAVLKKWEGLGYYSRARNLRRAAQILMEQGGAWP
                     RSAEAWATLPGVGPYTAAAIASICFGACEPVVDGNVIRVFARYLGWEDDFRTRLSREK
                     LAAWLRPHVMRSPAPGDFNQAMMDLGATVCTPRIPTCAVCPLRRSCQARREGRQTAYP
                     VKPKSQAIPVRHSIAARVRDARGRVLFTQRVDEGLLKGLWELPSVEVTNSATGRDAQR
                     ALRRLTGLRAARVRCVGTIRHAFSHFRLVMRVYELTEISGLLDTGRQPPARFAQPARL
                     PLTTATRRALTL"
     gene            complement(1351..1857)
                     /locus_tag="PLZ74_10595"
     CDS             complement(1351..1857)
                     /locus_tag="PLZ74_10595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR98806.1"
                     /translation="MHDPSDILIRPAILSDAMAIFTLIRNHPDDLIPRPIGDVIQNID
                     RFFVGEVEGRVVGCAAWKILPEIGKPERASVEIQSVAVDQAFRRRQVGTRIVGSILSA
                     IRRFQPAQAIVLTFAPEFFRALGFQIIPKTQVMHKLYMGCINCTKHADPFTCPEIAMA
                     LDLRQTAE"
     gene            complement(1996..>3342)
                     /locus_tag="PLZ74_10600"
     CDS             complement(1996..>3342)
                     /locus_tag="PLZ74_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOR98807.1"
                     /translation="ELRIFRGICLTVGMAAYGLALGGEPAGKTREAVFAASDLRQSPL
                     NEETLSETTSNGVVTTDFFFSGGEFNGQPSRIRAFYSRPEKKGVYPGVVQLHGAGLDV
                     LNSKASICYASNGFACISIDWCGPERKRKKPRNPPYSEFNEAGKQATVEVFPDEIGAN
                     GKKPRYDYHAVPVEADGIGAGVRFVRRSVMFLKSRPEVQHDQLFLSGMSAGAHLSLLV
                     LGVEPSFKGAAVKYGCAYIRDFPGYFGGYFGPLYLCPKPEQDAWLRVFEPRHGLPDYA
                     ASVLLLSGTDDIFFMMPLVLETYRNIPSEKRLLMLPNDNHSQVGNEILPARYFRSLLG
                     KAPAFPDAEAPTAEVVGQEIRLRCAVKVPSPLLKSVFYVKRMPVANFEFRKSKDVKWE
                     AVPTTLQKKVCSAMIPAPAEGEQVIAYLLLEDETGARISSDTVEIPAYPKWRGVTQ"
BASE COUNT          691 a         1076 c          966 g          609 t
ORIGIN      
        1 tcctagggct gatcgccttc agcttcggca cggccggtgg ttgctggatg ggccgtctga
       61 tgaacaagtt ctgcaaagaa ccgatcaacc ccttgatcgg ttcggcgggc gtctcggcgg
      121 tgccgatggc ggcgcgcgtg tccaacaagg tcggtctgga gtccgacccg aacaactacc
      181 tgctgatgca cgccatgggg ccgaacgtct cgggcgtgat cggttcggcg gtggtcgcgg
      241 gcgtgctcta cacgctctgt aaataaccgt ccgaacgtcg tcataacgtc aaggcgcgtc
      301 gagtcgcggt cgtgagtggc aaacgggcag gctgggcaaa ccgggccggc ggttgccggc
      361 ccgtatccag aagcccgctg atctccgtca gttcgtatac ccgcatgacg aggcggaagt
      421 gggagaatgc atgccggatc gtaccgacgc agcgaacacg ggccgcgcgc agaccggtca
      481 gacgtcggag cgcgcgctgg gcgtcgcgcc cggtcgcaga attcgtgacc tcaaccgaag
      541 gcagctccca caaccccttc aacaggcctt catcgacgcg ttgcgtgaac agtacgcgac
      601 ctctggcatc ccgcacccgc gcggcgatgc tgtgccggac cgggatcgcc tgagacttgg
      661 gtttcaccgg atatgccgtc tgtcgccctt cacgccgcgc ctgacaggaa cgccgcaacg
      721 gacaaaccgc gcatgttgga atgcgtggtg tgcacacggt cgcgccgaga tccatcatgg
      781 cctgattgaa atcgccgggc gccggcgacc gcatgacatg aggccgcagc catgcggcca
      841 gtttctcccg cgacagccgg gtccggaaat cgtcctccca tcccagataa cgggcaaaga
      901 cccggatcac gtttccgtcc accaccggct cgcacgcgcc aaaacaaatg ctggcgatcg
      961 ccgcagccgt ataaggaccc acccccggta gcgtcgccca tgcctccgca ctgcgcggcc
     1021 acgccccacc ttgttccata aggatctgcg cggcccgtcg caggttccgc gcccggctgt
     1081 aatatccgag tccctcccac tttttcaaaa ccgcctgctc gggagcctct gccagtgtgc
     1141 gtacggtcgg gaaagcctca agaaagcgca cgaaataccc acgtaccgta tcgacccggg
     1201 tttgctgcag catgatctcg ctgacccaga cggcatacgg atctggatgg tcgcgccacg
     1261 gcatctcgcg tcggtttatg cgataccacg acaacaaccg ttctgtcaga cttgggggca
     1321 tacccttccg ttcccggcgc ggctgacgtc tcactccgcc gtctgacgca gatccagcgc
     1381 catcgcgatt tcgggacagg taaagggatc ggcgtgtttg gtgcaattga tgcaccccat
     1441 gtacaatttg tgcatgacct gagttttcgg gatgatttga aaacccagcg cgcggaagaa
     1501 ttcaggcgcg aaggtaagca cgatcgcctg ggcgggctga aaccggcgga tcgcgcttag
     1561 gatggaaccc acgatccgcg tacccacttg acgccgccgg aaggcctgat ccaccgccac
     1621 ggactggatc tccaccgacg cgcgctcggg cttgccgatt tcgggcagga tcttccaagc
     1681 ggcgcatccc accacacggc cctccacctc ccccacaaaa aaacggtcga tgttctgaat
     1741 gacatcaccg atggggcgcg ggatcaggtc atccggatga ttgcggatca acgtgaaaat
     1801 cgccatcgca tcggacagga tggcgggacg gatcagaatg tcagagggat catgcatgtg
     1861 tgaaagatac gatactgatt gcggtccacc cgctcaagca gaaaccgcag gtgagcttat
     1921 tcgcggtcca cggtccgccg tgtgaccggt agaaaccgga ctggtgggcg ccagcggcac
     1981 ttcaggggcc tcgcttcact gtgtcacacc gcgccatttc ggataggcgg gaatctccac
     2041 cgtgtctgaa ctgattcgcg cccctgtctc atcttcgagt agcaggtagg cgatcacctg
     2101 ttctccctcg gcgggcgcgg ggatcatagc cgagcacacc ttcttttgca gtgtggtcgg
     2161 aaccgcctcc cacttcacgt ctttgctctt gcggaactcg aagttcgcga ccggcatccg
     2221 cttgacatag aacacgctct tgagcagcgg cgacggcacc ttgaccgcac accgcaaccg
     2281 gatctcttgt ccgacaacct cggccgttgg tgcctccgca tccgggaacg caggcgcctt
     2341 gcccagcaaa gagcgaaaat accgggccgg gagaatctcg ttaccgacct gactgtgatt
     2401 gtcgttgggc agcatcaaca gacgtttctc ggacgggata ttgcggtacg tttcgagcac
     2461 gagcggcatc atgaagaaaa tgtcatcggt tcccgaaagc agcaaaaccg acgccgcgta
     2521 atccggcagg ccgtggcgcg gctcgaaaac acgcaaccac gcatcctgct ccggcttcgg
     2581 acagaggtac agcggaccga aataaccgcc gaagtaaccg ggaaagtccc ggatatacgc
     2641 gcacccgtat ttcaccgccg cccctttaaa agacggctcc acgcccaaca cgagcagaga
     2701 taggtgcgcc cccgccgaca ttccggaaag aaagagctgg tcgtgttgca cctccggacg
     2761 gctcttcagg aacatcacag aacgccgaac gaagcggact ccggccccga tcccgtccgc
     2821 ctcgacaggc acggcatgat agtcgtaacg gggcttctta ccgttcgccc cgatctcgtc
     2881 gggaaagact tccacggttg cctgtttgcc cgcttcattg aactccgaat agggcggatt
     2941 ccggggcttt ttccgtttcc tttcgggacc gcaccaatcg atcgagatgc aggcaaagcc
     3001 gttcgaggca taacagatcg acgctttcga attgagaacg tccagccccg cgccatggag
     3061 ctgcacgaca ccgggataaa cccccttttt ctccgggcgt gagtaaaacg cgcgaatgcg
     3121 cgagggctgt ccgttgaact caccgccgga gaaaaagaag tctgtggtga ccacgccgtt
     3181 tgaggtcgtc tcggaaaggg tctcctcgtt caaaggggat tgcctcaaat ccgaggccgc
     3241 aaacacagcc tcacgcgttt ttccggcagg ctcacccccg agcgccagtc catacgccgc
     3301 catccccact gtcagacaaa tcccacgaaa tatcctcaat tc
//
LOCUS       DAOSRB010000270         3302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_26079_length_3302_cov_7.001232, whole genome shotgun sequence.
ACCESSION   DAOSRB010000270 DAOSRB010000000
VERSION     DAOSRB010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26079_length_3302_cov_7.001232"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLZ74_10605"
     CDS             complement(<1..359)
                     /locus_tag="PLZ74_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOR98808.1"
                     /translation="MDYRKEKGINMMQFNRRDFIRGSALAAASLVAGGCETTGAAGRY
                     PAVTRTVAPRFRKPGEKLNIGVVGVGGKGWSDWMPMFKNGENIVALCDVDRNPINKAL
                     AELEKAGVKPSQVKCYS"
     gene            458..1477
                     /locus_tag="PLZ74_10610"
     CDS             458..1477
                     /locus_tag="PLZ74_10610"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOR98809.1"
                     /translation="MNQKDRAFLAARVGRMEQMAAIRRLVEEDGRGRGMRVLDFESGS
                     GLAFSVYPDRGMDIGPARYKGIPLAWLACQPPAVPHAYDPEGCEWLRTWGGGLLTGCG
                     LLNVGGPCAADGERHGLHGRLSHLAAEEVNTSAAWTPDGTYTLSASGRMRHARVFGEN
                     LTLTRTISTAFDTPSITVRDTIENLGFAPTPVMLLYHMNLGWPLVDPEATLVMPDHPV
                     TPQNEHAAAGLAEWQRFAAPAPGFREQVYYHTLPADEQGLASAALVNPQLRLSLRVTY
                     RVAELPFLIQWKMCGQGEYVLGLEPANCYPEGREAAAQRDLLRTLEPGERLETFLRLS
                     VEPLA"
     gene            1483..3231
                     /locus_tag="PLZ74_10615"
     CDS             1483..3231
                     /locus_tag="PLZ74_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOR98810.1"
                     /translation="MPLPCTQREIRQLGWDAPDVILVSGDAYIDSPYSGVAVIGRVLE
                     REGFRTAILSQPDPASTEAFRSLGEPRLFWGVTSGCVDSMVANYTAAGRRRRQDDFTP
                     GGVNNARPDRAVIVYCNQIRAAFKPCKPIVIGGIEASLRRIAHYDCWSDTVRRPLLFD
                     AKADALVYGMGERTITALARAWRDGTDWRGLRGLCHALPASSQARPANAVALPDFNTV
                     AAPTPEGRSAFLEMFRLFATNQEAHTASPLLQKVDTRCLVHNPPAPPLTSAELDAVYA
                     LPFTLDPHPAVAARGPIRALDTIRFSLTTHRGCYGACRFCAIAMHQGRQVVSRSQSAI
                     LDEARRMTRHPAFTGILRDVGGPTANMYGFDCSRKTLQGACPDRECLFPACCDSLRPN
                     HGPQRELLASLRRLPGVRKVFVASGIRPDLIAADPRHGDGYIDDLAAHHVSGQIKLAP
                     EHTEPRVLAAMGKPGLDALLTFKRKFEAANRRHGLRQFLTYYFIAAHPGCTDDDMRRL
                     RRFVASHLHLNPEQVQIFTPTPSTWSTAMYYTGYDPFSGTPIHVTRNPRDKQRQKAIL
                     APDAARARNLVITKRG"
BASE COUNT          580 a         1204 c          941 g          577 t
ORIGIN      
        1 tcgctgtagc acttgacctg cgagggtttg acccccgctt tctccaactc ggccaaggcc
       61 ttgttgatcg gattgcggtc cacgtcgcag agcgccacaa tgttctcgcc gttcttgaac
      121 atcggcatcc agtcgctcca tcccttgccg cccacaccga ccacgccgat attgagcttc
      181 tcgcccggct tacggaatct cggcgcgacc gtgcgcgtca ccgccggata gcggcccgcc
      241 gctccggtgg tctcgcatcc gcccgcgacc agcgacgccg ccgccagtgc cgatcccctg
      301 ataaaatcac gacgattgaa ctgcatcatg ttgatcccct tttcctttct gtaatccatc
      361 caccttcttc atacaaacaa aacaggaaaa gagtttaatt cgtttcgtgc cgaaaaagca
      421 actctttgct acgattattt caaatcggag gaagaggatg aatcaaaaag accgtgcttt
      481 tttagcggcc cgcgtgggcc ggatggaaca aatggccgcg attcggcggt tggtggagga
      541 ggacggccgg ggacgcggca tgcgcgtact ggacttcgaa tccggcagcg gactcgcgtt
      601 ttccgtgtat ccggaccgcg gcatggatat cggcccggcg cggtacaaag gcatcccgct
      661 ggcctggctc gcatgccagc cgcccgccgt gccccacgcc tacgaccccg agggatgcga
      721 gtggctgcgg acctggggcg gcggcctgct caccggttgc ggcctgctga atgtcggcgg
      781 accgtgcgca gcggacggcg aacgccacgg tctgcacggc cggttgagcc acctcgcagc
      841 cgaggaggtg aacacctccg cagcctggac gccagacggc acgtataccc tgagcgccag
      901 cggacgcatg cgtcacgccc gggtcttcgg cgagaacctc acgctcacgc gcacgatctc
      961 gaccgccttc gacacgccgt ccatcaccgt gcgcgacacg atcgagaatc tgggcttcgc
     1021 gcccacgccc gtgatgctgc tctaccacat gaacctcggc tggccgctgg tggacccgga
     1081 agccacactt gtgatgcccg accatcccgt gaccccgcag aacgagcatg ccgcggccgg
     1141 gctcgcggag tggcagcgtt tcgccgctcc cgcgccgggc ttccgcgaac aggtctacta
     1201 ccacaccctg ccagccgacg aacaggggct ggcctcggcc gcgttggtga atccgcagtt
     1261 gcgcctctcc ctgcgcgtga cctaccgcgt ggcagaactc cctttcctga ttcagtggaa
     1321 aatgtgcggc caaggcgaat acgttctggg gcttgaaccc gccaactgtt accccgaggg
     1381 ccgggaggcc gcagcccaac gcgatctctt gcgcacactt gaaccgggtg aacgtctgga
     1441 aacgttcctg cgcctctcgg tcgaaccgct cgcctaagcc ccatgcccct gccgtgcaca
     1501 cagcgggaga tccggcaact cggctgggac gcgccggatg tgattctggt ctcgggtgac
     1561 gcctacatcg attcgcccta ttccggcgtg gccgtgatcg gtcgtgtgct cgaacgcgaa
     1621 ggcttccgca ccgcgatcct gtcccagccc gatcccgcct cgacggaggc cttccgcagc
     1681 ctcggcgaac cgcgcctctt ctggggcgtc acaagcggat gcgtggattc catggtcgcc
     1741 aactacaccg ccgccggccg ccgccgcaga caggatgact tcactcccgg cggcgtcaac
     1801 aacgcccggc ccgaccgcgc ggtgatcgtc tactgcaacc aaatccgcgc cgccttcaag
     1861 ccctgcaaac cgatcgtcat cggcggcatc gaggccagcc tgcgacggat cgcgcattac
     1921 gactgttggt ccgataccgt gcggcgcccc ctgctgttcg acgccaaagc tgatgcgctc
     1981 gtctacggta tgggggagcg cacaatcacg gcgttggccc gcgcatggcg tgacggtacc
     2041 gactggcgcg gcttgcgcgg cctctgccac gccctgccgg cctcatcgca ggcacggccc
     2101 gcaaacgctg tcgctcttcc ggatttcaac accgtggccg cgcccacccc ggaagggcga
     2161 tccgcctttc tggagatgtt ccgcctcttc gcaaccaacc aagaggctca cacggccagt
     2221 cccctcctgc aaaaagtgga cacccggtgc ctggtgcaca acccgcctgc gccgcccctc
     2281 acctcggctg agctggatgc cgtctacgcc ctgccgttca cgctcgaccc ccatcccgct
     2341 gtggcggccc gcggccccat ccgcgcactg gacaccatcc gtttctcgct gaccacccac
     2401 cgcggctgtt acggggcgtg ccgcttctgc gccatcgcca tgcaccaggg tcgccaagtg
     2461 gtcagccgct cacaatcggc gatcctcgac gaggcccgcc gcatgacacg ccatccggcg
     2521 ttcaccggca tcctccgcga cgtgggcgga cccaccgcca acatgtacgg cttcgactgt
     2581 tcgcgcaaaa cgctccaagg cgcctgcccc gaccgggaat gcctcttccc cgcgtgctgt
     2641 gactcgctgc gccccaacca cggaccgcag cgggaactgc tcgccagcct acgccgcctg
     2701 cccggcgtgc gcaaggtgtt cgtggcctcc ggcatccggc cggacctgat tgccgcagac
     2761 ccgcgtcacg gcgacggcta catcgatgat ctcgcggccc atcatgtctc gggacagatc
     2821 aaactcgcgc ccgagcacac cgaaccgcgg gtgctcgccg ccatgggcaa acccggtctc
     2881 gacgcgcttc tgaccttcaa acggaaattc gaggccgcca atcgcaggca cggcctccgg
     2941 caattcctca cttactactt catcgctgcg caccctggct gcaccgatga cgacatgcgc
     3001 cgcctgcggc ggtttgtcgc ctcgcatttg cacctcaacc ccgagcaggt gcagatcttc
     3061 accccgacgc cctctacttg gtctaccgcg atgtactaca ccggttacga cccgttcagc
     3121 ggcaccccta tccacgtcac ccggaacccg cgcgacaaac agcggcaaaa ggcgattctg
     3181 gcacccgacg cggcgagagc ccgtaacctc gtcatcacga aacggggata atacaagacg
     3241 taacctaaaa agagcagttg aaccttcaac tctccttggt ttcaaggttt cggggtttct
     3301 gg
//
LOCUS       DAOSRB010000271         3298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_26128_length_3298_cov_6.247610, whole genome shotgun sequence.
ACCESSION   DAOSRB010000271 DAOSRB010000000
VERSION     DAOSRB010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26128_length_3298_cov_6.247610"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLZ74_10620"
     CDS             <1..201
                     /locus_tag="PLZ74_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98811.1"
                     /translation="FPIFMKFHVFHGSIPIGIGIAIGIDCLTTEHFLGFLGFWFHVMG
                     GTPMPVLFISPISADCGNLIHH"
     gene            292..>1345
                     /gene="mnmE"
                     /locus_tag="PLZ74_10625"
     CDS             292..>1345
                     /gene="mnmE"
                     /locus_tag="PLZ74_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOR98812.1"
                     /translation="MNFPTSTDTIAALATAPGAAGVCVVRVSGPGALALGDRLTPSAR
                     PAPSRRPAGTFFHAVIAHPLTGERLDDALVLVFRAPRSFTGEDTIEIQGHGGLLPPRR
                     LLEAALAAGARLAEPGEFTRRAFLNGRLDLTQAEALCDFIGAKTERAAQVARAQLDGA
                     LGREVGRRYEELTGMCAAIEHLLDFDENEPPPGAAEAYAAALTPLADALRDLAGSWER
                     GHLLRDGALVVICGRPNAGKSSLLNALLGRDRAIVHDAPGTTRDVIEEGYALCGVPLR
                     LVDTAGLRSAADAVEREGVRRAQALIRQADLILYLVDRSAASDLSDLSDSETCNPHLF
                     PPERTLLCLTKCDLPPA"
     assembly_gap    1346..1445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1446..1933
                     /locus_tag="PLZ74_10630"
     CDS             <1446..1933
                     /locus_tag="PLZ74_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98813.1"
                     /translation="QPAHCPLPLPTAPANSSTSLLSTVTGERTLLCLTKCDLPPAPQP
                     AHCPLPLPTAPANSSTFLLSTVTGEGLSELKQAIIRSLGLEREPCGQQVVSLRQAEEL
                     REAARQSLAARDALEAGPEGFVLAAGHARHAAEALGRILGRVYSDDLLDAIFRRFCVG
                     K"
     gene            2731..>3298
                     /locus_tag="PLZ74_10635"
     CDS             2731..>3298
                     /locus_tag="PLZ74_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98814.1"
                     /translation="MMMKKLVIALAACVMAGAVFAQLTSQIVGYNTVTVYPGYNLLAV
                     NFDGVGAGSLTLAELFPTTDGEGNLLPGFTAGNAGTGDNIRFYDASTEGYTAYYLYKG
                     AKTSDTKNYKWVTTAGVVASATPVDSGTAFWYKKIATTTNAVSFAGQVANDVSETHQI
                     LTGYNMFSSIYPAAWNPNDLGVAYWQNSG"
BASE COUNT          595 a         1052 c          925 g          626 t
ORIGIN      
        1 ttcccgattt tcatgaagtt tcatgtcttt catggttcca ttccaatcgg gatcggtatc
       61 gctatcggta tcgactgttt aaccacggaa cactttcttg ggtttctggg tttctggttt
      121 catgtcatgg gcgggacgcc catgccagtg cttttcatct ccccaatctc tgccgattgc
      181 ggtaacctta ttcatcactg acgcactgac gcactaccgc actgacgcac taccgcactg
      241 acgcactacc gcactaatgc actaatgcac taatgaacta atgaactaat aatgaacttc
      301 cccacctcca ccgataccat tgccgcgctt gccaccgcgc cgggggcggc gggtgtgtgc
      361 gttgtgcgtg tttcagggcc cggggcgctg gcccttggcg accgcctgac gccttccgcg
      421 cgtcccgcgc cttcgcggcg cccggccggg accttcttcc acgcggtgat cgcccacccg
      481 ctgaccggcg aacggctgga tgacgcgctg gtgctggttt tccgcgcgcc gcgcagcttc
      541 accggcgagg acacgatcga aatccagggc cacggcggtc tccttccccc gcgccgcctg
      601 ctcgaggccg cgctggccgc gggcgcgcgc ctcgccgagc cgggcgagtt cacgcgccgc
      661 gcttttctca acggccgcct cgacctgacg caggccgagg ccctctgcga tttcatcggc
      721 gcgaaaaccg aacgcgccgc gcaggtggcc cgcgcgcaac tcgacggcgc gctcggccgc
      781 gaggtcggcc gccgctacga ggagctgacc ggcatgtgcg ccgcgatcga gcacctgctg
      841 gatttcgacg agaacgagcc gccccccggt gccgcggagg cctacgccgc ggccctgacg
      901 ccgctcgcgg acgcgctccg cgacctggcc ggatcatggg agcgcggtca cctgctgcgc
      961 gacggcgcgc tggtggtgat ctgcggccgc cccaacgctg gcaaatcctc gctcctcaac
     1021 gccctgctcg gccgcgaccg cgcgatcgtc cacgacgcgc ccggcaccac gcgcgacgtg
     1081 atcgaagagg gctacgcgct ctgcggcgtg ccgctgcgcc tggtggacac cgcgggcctg
     1141 cgctccgccg ccgacgcggt cgagcgcgag ggggtccgcc gcgcgcaggc cctgatccgc
     1201 caggcggatc tgatcctgta tctggtggac cggtctgcgg cgtccgatct gtccgacttg
     1261 tccgactccg aaacctgcaa cccgcacctc ttccctccgg agcgcacgct gctctgcctg
     1321 accaaatgcg acctgccacc tgcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnncccaa ccagcccact gcccactgcc actgcccact gctcctgcca actcctccac
     1501 ttccctcctt tccaccgtca cgggcgagcg cacgctgctc tgcctgacca aatgcgacct
     1561 gccacctgcg ccccaaccag cccactgccc actgccactg cccactgctc ctgccaactc
     1621 ctccaccttc ctcctttcca ccgtcacggg cgaaggcctt tccgaactca agcaagccat
     1681 catccgcagc ctcggactgg agcgcgaacc ctgcggccag caggtggttt ccctgcgtca
     1741 ggccgaagaa ctgcgcgaag ccgcgcgcca gagcctcgcg gcccgcgacg cgctcgaagc
     1801 cggccccgag gggttcgtgc tcgccgccgg gcacgcccgc cacgctgccg aagccctcgg
     1861 ccgcatcctc ggccgcgtct acagcgacga cctccttgac gccattttcc gacgcttttg
     1921 cgtcgggaaa taggctgtta tgaggctgtg agggtgttag gctgtgaggc tgtgagggtg
     1981 tatcgatccc gattccgata gcgagagcga tcccgatttg gatgaaaccg tgaaaccccg
     2041 aaaccctgaa accgtgaaac cgtgaaaccc tgaaaccccg aaaccaaaga gaaacgcact
     2101 ttttcccaac tcctcttccc cctctggaaa aaattttaat ttttcctctt tttccccttg
     2161 catcggttga cgcttttggc gtatagtaac gctgtcgtaa aaaagtcggg aaaggtcccg
     2221 cgggtgcggt ggcccttccg ttaagaggga gagagtggtt ttttacagga gtttccgggc
     2281 gactggcgcg aagcgccggt ccgggacagg ccgggctcgc aaagcgtttg acaggctgcc
     2341 gcgcgatacc gaagggttgg ttggacattc tttcgttgta ttcgttgtac ccttcggtgt
     2401 ggcttctggc ggaaaagaga acgcggacgg acacggccgg ggcgtgcgag agcgggaagg
     2461 acggatgcgg gatttggccg cgtttgccca ccgtgcgcaa acccccgacc cggggcgcca
     2521 aaaaaatcat ctttttccaa gaaaggttgt tgacgcggcg gttgccctcg ggtaaactgt
     2581 ccccgtcttt gagtaggaaa agaactccga aacaaacgag gaaggaagag agacaaaaga
     2641 ggctgacgga ttactggaag aaatggggtg ctgacaactc ctaaatccta aatcctaaat
     2701 cctaaattga atcctgactt cctttggagg ttgatgatga aaaagttagt gattgctctt
     2761 gcagcatgtg tgatggctgg cgcggtgttc gcgcagctca caagccaaat cgtcggatac
     2821 aacaccgtca cggtttatcc gggttataac cttttggcgg tgaatttcga tggcgtcggc
     2881 gcggggagtt tgacgcttgc cgaacttttc cccaccacgg atggggaagg caatctcctt
     2941 cccggcttta cggcgggaaa tgccggtacg ggtgacaaca tccggtttta tgacgcgtca
     3001 accgaagggt atacagccta ttacctgtac aagggcgcca agacctcgga caccaagaac
     3061 tataaatggg tgaccaccgc cggtgttgtc gcttcagcta ctcccgtcga ttcaggaacc
     3121 gctttctggt ataaaaaaat agcgacaacc acgaatgcgg tttccttcgc cggccaggtc
     3181 gcgaacgatg tgtcggaaac ccaccagatc ctgacgggct acaacatgtt cagttcgatt
     3241 tatccggccg cgtggaatcc gaacgatctg ggagtcgcct attggcagaa cagcgggg
//
LOCUS       DAOSRB010000272         3270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_26434_length_3270_cov_6.287403, whole genome shotgun sequence.
ACCESSION   DAOSRB010000272 DAOSRB010000000
VERSION     DAOSRB010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26434_length_3270_cov_6.287403"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..500
                     /locus_tag="PLZ74_10640"
     CDS             279..500
                     /locus_tag="PLZ74_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOR98815.1"
                     /translation="MLRVIAALERENIEARPVWKPMHLQPVFRGVRIYGGAVSERLFA
                     DGLCLPSGSGLERAEVIRIGEVIGNCLIG"
     gene            525..2420
                     /locus_tag="PLZ74_10645"
     CDS             525..2420
                     /locus_tag="PLZ74_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOR98816.1"
                     /translation="MKKEWQERFGVGLSRLGCFLLDTAVMAVAYLTAFILRFDFAEPH
                     WGWGGVARSFITVWAVQMVALWLFGCRRLLWRYVSVGDVPQFVCAGFFSTLVLVLLRM
                     TLPGHLSLRPPYSITFFNSFLVTGGLLGVRLLWRLMVEGFSGAREEVSPKGTRTARRV
                     RRVLLVGAGEAGNLVARELKRQGGRRQRVVGFLDDDPAKRHARIQGFPVVGEIRDLPK
                     AAHKYAVDEVVIAMVRVPRNVIRRVVRLCERAHVPARIVPGYYELIEGSLTASKIRDI
                     DVSDLLGREEATTDSGPVVSLFTQKRVLITGAGGSIGSELVRQVLRTGPGLLVLVDRS
                     ENALYEIEREIRKLGVATPVQAVLADVGDARRMQMVFRQVKPQLVVHSAAHKHVPLME
                     QNPVEAVKNNVLATRALGELAVACGTERFVLISTDKAVKPISVMGMTKRLAEAALQEL
                     NTRGQTRFSAVRFGNVLGSSGSVVPLFREQIRKGEAVTVTHPEMRRYFMTIAEAVALV
                     LQAMALAEGGEVFVLDMGEPVRIVELAEEMIALSGLRPYEDIPIVFTSIRPGEKLFEE
                     LDVSERSAYKTGHAQIFVSKTAPADTARVEGVLAACRRMVEKEAGADEVREALQEMVR
                     DIGKDVS"
     gene            2417..>3270
                     /locus_tag="PLZ74_10650"
     CDS             2417..>3270
                     /locus_tag="PLZ74_10650"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOR98817.1"
                     /translation="MKRPLAWTRVIVCGIIAVAALIHYRPMFVVTAAMFAEPLEDMSH
                     GWLVPLFSLYVLWRQRHALREAAGRPDRVGAVWVALFLVIAWFGSRGGQSRMEQVSFI
                     GLVWSVPYALWGRRVERLMRFPAVFLFFTVPLSSFLDFFTIHLRLVTSVLATGILNGL
                     GLAVERSGTALFSRVPGGSFNVDVADPCSGIRSLFALMALTAAYAYFSQRSLWRKWAL
                     FACSLPIAMIGNMVRIMSICLIARWFGQEAATGYYHDYSGYVVFLVGVLLMVQAGDWI
                     GKGEKGGKG"
BASE COUNT          558 a          713 c         1245 g          754 t
ORIGIN      
        1 aaagggaatg gcggccaccc agtggtcgca atggggagag taaaagggaa tggcggccac
       61 ccagtggtcg caatggggag ggtaaaaggg aatggcggcc acccagtggt cgcaatgggg
      121 agagtaaaag ggaatggcga ccacccagtg gtcgcaatgg ggagagtaaa agggaatggc
      181 agccacccag tggtcgcaat ggggagggta aaagggaatg gcagccaccc agtggtcgca
      241 atggggaggg taaaaacggg cacggtccgg gcgaacaggt gctgagggtg atcgcggcgt
      301 tggaacggga gaacatcgaa gcgcggcctg tctggaagcc gatgcatctg cagcccgtgt
      361 tcaggggtgt ccggatttat gggggtgcgg tcagcgagcg tctcttcgcc gacgggctct
      421 gtctgccgtc gggtagcggg ttagagcggg cggaggtgat ccgcatcggt gaagtgatag
      481 ggaattgtct gatagggtag attgtggtta aatttacaaa ccgtatgaaa aaagagtggc
      541 aagaacgttt cggagtcgga ttgagcaggc tcggatgttt tctgctggat acggcggtta
      601 tggcggtcgc gtatttgacc gctttcattc tgcgttttga ttttgcggag ccgcattggg
      661 gatggggggg tgtggcacga tctttcatca cggtgtgggc ggtgcaaatg gtggcgttgt
      721 ggcttttcgg gtgcaggagg cttttgtggc gttatgtgag tgtcggggat gtcccgcaat
      781 ttgtctgcgc tggttttttt tcgacccttg ttctggtgtt gttgcggatg acgttgccgg
      841 ggcacctgtc cttgcgcccc ccttatagta tcacgttttt caacagtttc ctggtcacgg
      901 gcggattgtt gggtgtacgg ttgctgtggc gcctgatggt tgagggtttt tctggggcgc
      961 gggaggaggt gtcgccgaag ggaacccgca cagcgagacg ggtgaggcgg gtgttgttgg
     1021 tcggggccgg tgaggccggg aatctggtgg cgcgcgagtt gaagcgtcag ggcggcaggc
     1081 gtcagcgggt ggtggggttt ctggatgacg atccagctaa gcggcatgcc cggatacagg
     1141 ggtttccggt ggtgggagag atccgggatc tgcccaaggc ggcacacaaa tacgcggtgg
     1201 atgaggtggt catcgcgatg gtgcgggtgc cccggaatgt gatccggagg gtggtgcggt
     1261 tgtgcgagcg ggcgcatgtg ccggcgcgga ttgtgccggg gtattatgag ttgatcgagg
     1321 ggagcctgac cgcatccaag atccgggata tcgatgtatc ggatctgttg gggcgtgaag
     1381 aggccacaac ggacagcggc cccgtggttt cgctgttcac gcaaaagcgg gttctgatta
     1441 cgggggccgg cggatcgatc gggagcgaat tggtcagaca ggttctccgc acgggaccgg
     1501 ggttgcttgt gctggtcgac cggtcggaga acgccttata tgaaatcgaa cgcgagatcc
     1561 ggaaactggg tgtggccacg ccggttcagg cggtgttggc ggatgtgggg gatgcgcgac
     1621 gaatgcaaat ggttttccgt caagtgaagc ctcaacttgt ggtgcattcc gctgctcaca
     1681 agcatgtgcc gctgatggaa cagaatccgg ttgaagcggt gaagaacaat gtgttggcga
     1741 cgcgggcgct gggcgaactg gccgtcgcgt gcggtacgga acggtttgtt ctgatttcga
     1801 ccgacaaagc ggtcaagccg atctcggtga tggggatgac caaacggttg gcggaggcgg
     1861 cgttgcagga gttgaacaca cgcgggcaga cgcgtttttc cgccgtacgg ttcgggaatg
     1921 tgctgggatc atcgggttcc gtcgtgccgc tgttccgcga gcagatccgg aaaggggagg
     1981 cggtgacggt cacgcatccg gagatgcggc ggtatttcat gacgattgcg gaagcggtgg
     2041 cgctcgtgct gcaggcgatg gccttggcag aagggggaga ggtttttgtt ttggatatgg
     2101 gggagccggt gcggatcgtg gaactggctg aagagatgat cgcgctttcg gggttgcggc
     2161 cctatgagga cattccgatc gtgttcacaa gcatccgtcc cggggaaaaa ttgtttgaag
     2221 agttggatgt gagcgaacgt tcggcttaca agactgggca tgcgcagatt tttgtgagta
     2281 agacggcgcc tgcggatacc gcgcgtgtcg aaggggtgtt ggcggcttgc cgccgcatgg
     2341 tggagaagga agcgggggcg gacgaggtgc gggaggcctt gcaggagatg gttcgcgaca
     2401 tcggaaagga tgtatcatga agcgacctct cgcatggacc cgggtgattg tttgcgggat
     2461 cattgccgtg gcggcgctga ttcactaccg cccgatgttt gtggtcacgg ccgcgatgtt
     2521 tgccgagccg ttggaagaca tgtcgcacgg gtggctggtt ccgctgttca gcctgtatgt
     2581 gctgtggcgt cagcgtcacg ctttgcgcga ggcggccgga cggcccgaca gggtgggggc
     2641 ggtgtgggtg gcgttgtttt tggtgatcgc gtggttcggt agccgcggcg ggcaatcgcg
     2701 catggagcag gtttcgttca tcgggttggt gtggtctgtg ccgtatgctt tgtggggacg
     2761 ccgggtggag cggctcatgc gttttcctgc cgtgttcctc ttctttaccg ttccgctctc
     2821 atcctttctg gatttcttca cgatacatct gcggctcgtc acttcggtgc tggccaccgg
     2881 tatcttaaat ggtttgggtt tggcggtgga gcggtccggt accgcgcttt tctcccgcgt
     2941 gcccggcggg tccttcaatg tggatgtggc ggacccgtgc agcgggatcc ggtctttgtt
     3001 cgcgttgatg gcgctgacgg cggcgtatgc gtatttttcg cagagaagct tgtggcgtaa
     3061 atgggcgctg ttcgcctgtt ccctgccgat cgccatgatc gggaacatgg ttcgcatcat
     3121 gtccatctgt ctgatcgcgc gttggttcgg acaggaagcc gcaaccgggt attatcatga
     3181 ttattccggg tatgtggtgt ttctggtcgg ggttctgctg atggtgcagg ctggcgactg
     3241 gatcgggaag ggggagaagg gggggaaggg
//
LOCUS       DAOSRB010000273         3232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_26862_length_3232_cov_5.921939, whole genome shotgun sequence.
ACCESSION   DAOSRB010000273 DAOSRB010000000
VERSION     DAOSRB010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26862_length_3232_cov_5.921939"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(319..1494)
                     /locus_tag="PLZ74_10655"
     CDS             complement(319..1494)
                     /locus_tag="PLZ74_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HOR98818.1"
                     /translation="MKGILTKLPKVALFLATHEKGGRDKLQGVLRYVRLHTPWRIHLI
                     EPRAVGQQLVDLKAWGVTGVIVSRMPEALRFVARAGVPTVIMDSPARYPQSHANISFV
                     TSDSEAVGRAGAEIFLKQGFTNFAYVPDAYDWDWSVLRGQAFRARLEQAGYTCSLYGP
                     LTVRERKDWTLDQRRLVRWLAGLVKPVALMAACDNRARQVLETCQTAGFDVPGEVALL
                     GVDNDELLCENTVPTLSSVQPDFEAGGYEAARLLEQLMRRTVRAPEPILYGVKRVVSR
                     ASGRYAHAVDRRFLRGIEFVRLNACAVIRVPDIARHMGVSRRTAEVLFRRHAGHGVRE
                     EIQQVRLSRLKTFLLETGLPIGQISAQCGFQTEMHAKRVFKQATGMTMRQFRSRNAS"
     gene            1666..3063
                     /locus_tag="PLZ74_10660"
     CDS             1666..3063
                     /locus_tag="PLZ74_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98819.1"
                     /translation="MNRRHFLAASALGAAGFFSGCATAPQPRPLAPNSKLNHACIGVG
                     GMGGADISNIKTHANTQIVALCDVDRTRLEHAAKAFPGARTYTDWRELLAKEGDRIDS
                     VNVSVPDHNHTIIATHAMRAGKHVYCQKPLCHEIAECRLLKETARRHGVVTQLGVQHS
                     AGVGDRMAVAYHQAGVIGAVEHIYIFSNRKGISRIRVLRPEKADPVPASLDWDLWIGT
                     APFRPYAEKIYHPLIWRIWQDFGSAWIGDIGCHLFSSIWKGIGLTTPISVKAEVQEEW
                     KREPARFKDTWPQGAHITWVFPGVKASDGKPITVEWFDGMTGEAAPNLLPPPELHELA
                     KEVGLERLPNEGSVVAGSEGWLILPHSAGPRLVFKNRQAAKPPKPVLPNWPNHYHEFL
                     DGCVAGRRTSADFATVASMVEAVLLGNVAERVPDTLLKWNARRMCITNSQEATRYLRR
                     AYRKGWELDGLETVS"
BASE COUNT          665 a         1043 c          930 g          594 t
ORIGIN      
        1 ctgaagaaaa ggtcttacaa caaggaactt tccccactaa cgatttcaac gttgaagatt
       61 ttcagcatgg tcatcaacgg acgagaatcg ccgcggccaa ctccctccgg gcgtcggaca
      121 gggctttcgg atcgcgtgcg tacccccacc atgtcggggc acggccggtc gatgtgccaa
      181 tagggcggat ccttctcgcg tacccctcgc ccaccagttc ggaaagtgtg acggaggtca
      241 cccgcaggtt ttgaccccgt gcggccacga tcactgaaac gattcgacct cgttacgtgc
      301 gtttgcaaga aaacagggtt atgacgcgtt acggcttcga aattgacgca tcgtcattcc
      361 ggtcgcctgc ttgaacacgc gttttgcgtg catctcggtc tgaaagccgc attgggcgct
      421 gatctgtccg atcggtaggc cggtctccag cagaaaggtc ttgaggcgcg acagacgaac
      481 ctgctggatc tcctcgcgca ccccgtgtcc cgcatggcgt cggaacagca cttcagccgt
      541 ccggcgggag actcccatgt gacgcgcgat gtccggtacg cggatcactg cacaggcgtt
      601 gagccggacg aattcgatcc cccggaggaa acgccggtcc accgcatgcg cataccggcc
      661 ggaagcacgc gaaacaacac gcttcacccc atacaggatc ggctccggcg cgcgcaccgt
      721 gcgccgcatg agttgttcca gcaaccgggc cgcctcgtat ccccccgcct cgaaatcggg
      781 ctggacactg gaaagcgtgg gcaccgtgtt ctcgcacagt agctcgtcgt tgtccacccc
      841 aagcagggcg acctcccccg gcacgtcgaa cccggcggtc tgacaggttt ccagaacctg
      901 ccgcgcccgg ttgtcacagg ccgccatcag ggccacaggt ttgacgagtc cggcaagcca
      961 gcgcaccaga cgccgctggt ccagcgtcca atccttgcgc tcgcgaaccg tgagcgggcc
     1021 gtacagggaa caggtgtaac cggcctgctc caaccgcgcc cggaacgcct gaccccgcaa
     1081 taccgaccaa tcccagtcat acgcgtccgg cacatacgcg aaattcgtaa aaccctgttt
     1141 taaaaagatc tcggccccgg cacggccgac cgcctccgaa tcactggtca caaatgaaat
     1201 attcgcgtga ctctggggat agcgcgccgg agagtccatg atcacggtcg gcacgccagc
     1261 gcgcgccaca aaccggaggg cctcgggcat gcggctcacg atcacgccgg tcacacccca
     1321 tgccttgaga tcaacgagtt gctgtccgac ggcacgcggc tcaatcagat ggatgcgcca
     1381 cggcgtgtgc agccggacat aacgtaatac accttgaagc ttgtcgcgcc cccccttttc
     1441 gtgggtggcg agaaacaacg ccacctttgg caacttcgtc agaatccctt tcacagaccc
     1501 ctccctttct aaccgtggcc acaggcgaaa aaggaagtat accatctgcc acttcagtac
     1561 gacaagacaa gggtctgcgc aaaatgcagt atttttttta cgcattaggc ggtatacttt
     1621 tctgcaggtc tgctattcta gtgttaaatg aatggagagc ataacatgaa ccgtcgtcat
     1681 tttcttgccg catcggcact gggtgccgca ggctttttca gtggctgcgc cactgcaccg
     1741 caaccgcgcc cgctcgcgcc caacagcaag ctcaatcacg cttgcatcgg cgtgggcggc
     1801 atgggcggcg cggacatcag caacatcaag acgcatgcca acacccagat cgtggcgctt
     1861 tgcgatgtcg accgcacacg tctcgaacat gcggccaaag cctttcccgg cgcgcgcacc
     1921 tataccgact ggcgcgagct gctggccaag gagggcgacc gcatcgattc ggtcaacgtc
     1981 tcggtgcccg accacaacca cacgatcatc gcgacccacg ccatgcgcgc gggcaagcac
     2041 gtctactgcc agaagccgct ttgccacgaa atcgcggagt gccgcctgct caaggagacc
     2101 gcccggcgtc acggcgtcgt cacgcaactc ggcgtccagc attcggcggg cgtgggcgac
     2161 cgcatggccg tggcctacca tcaagccggc gtgatcggcg cggtcgaaca catctacatc
     2221 ttctctaacc gtaagggtat ttcgcgtatc cgtgtgctgc gccctgaaaa agctgatccc
     2281 gtgcccgcct cgctcgattg ggatctgtgg atcggcaccg caccgttccg tccctacgcg
     2341 gagaaaatct accacccgct catctggcgt atctggcagg acttcggttc cgcctggatc
     2401 ggcgacatcg gctgccatct gttcagttcg atctggaaag gcattgggct gaccacgccg
     2461 atttccgtca aagccgaggt gcaggaggag tggaaacgcg agccggcccg gttcaaggac
     2521 acctggccgc agggcgcaca catcacctgg gttttccccg gcgtcaaggc cagtgacggc
     2581 aaaccgataa ccgtggagtg gtttgacggc atgaccggcg aggccgcgcc caatctgctg
     2641 ccgccgccgg aactccacga gctggccaaa gaggtcgggc tggagcggtt gcctaatgaa
     2701 ggctcggtcg tggccggttc ggaaggctgg ctgatcctgc cgcactcggc gggtccgcgt
     2761 cttgtattca aaaaccggca ggccgccaaa cctcccaaac cggttttacc gaactggccc
     2821 aaccattatc acgaatttct ggacggctgt gtggcgggac gccgcacgag cgcggatttc
     2881 gcgaccgtcg cctcgatggt cgaagccgtg ctacttggca atgtcgccga acgtgtgcca
     2941 gacacactgc tgaagtggaa cgcgcgtcgt atgtgcatca cgaactcaca ggaggcgaca
     3001 cgttatctac gccgcgctta tcgtaagggt tgggagcttg acggcttgga gaccgtttca
     3061 tgattaactt cgatacacgt gaaaaacggc cgcacatgga tatcaataga aggcagtttt
     3121 taacattcag cgctgcggga ttgggcggtg cggcattgtt cggcgcacgg caggcgtacg
     3181 ccgccaccac gagcggcatg ccggttctca aggcacccga accgcccgcc ga
//
LOCUS       DAOSRB010000274         3218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_27022_length_3218_cov_5.352830, whole genome shotgun sequence.
ACCESSION   DAOSRB010000274 DAOSRB010000000
VERSION     DAOSRB010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27022_length_3218_cov_5.352830"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1229
                     /locus_tag="PLZ74_10665"
     CDS             165..1229
                     /locus_tag="PLZ74_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98820.1"
                     /translation="MIRTTRPPIRVGLVGLGRAGLEMHVPELEQYPELFKIIAVCDPV
                     KERRDLAVERIPECRTYRRYEDLLADPDVELVDIATRSDEHAAHGMQALKTDKWVNME
                     RPFCRDYDEALVLRAAAIKASNRLLVRHNYRFEPAFMQVREVIESGVLGELYDIKIRR
                     GVYERRDDWQTVKRCGGGAALAWGTAFLDQALELLRTPPIKVWSDFKRVASVGDAEDY
                     VRIILRNMAGLTVDLEVSGGRIGEMPQFIVTGTKGAFTLYPDAEQGTLRYLDPKQKLA
                     RRRSSVRTPPLGSFGTAETLQWLETSVPVKPKAECGMSLIWKYVYQAIRENKPYPVPL
                     DVAIETMRILSLVKKESPFA"
     gene            complement(1242..2879)
                     /gene="ade"
                     /locus_tag="PLZ74_10670"
     CDS             complement(1242..2879)
                     /gene="ade"
                     /locus_tag="PLZ74_10670"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOR98821.1"
                     /translation="MSLSFCGTLLDPVQNTAYGARLTVSDTGVIDTITPEPDPDPGFI
                     MPGLVDAHVHLESSMLTPAAFARVAVRHGTLAAVVDPHEIANVLGEAGVELMLRLTKQ
                     TPFVFGFGVPSCVPATPFETAGATLDSDAVTRLLGLPGITHLSEMMNVPGVLNEDPEV
                     RAKLDAARARGLPIDGHAPGLTGEAMRTYANAGITTDHESLSFDEAREKIRGGIQLQI
                     RYGSATHLFEPFLPLLTRHPEHCMFCSDDKHPDDLLHDHINTIAAKAYTRGIPLQAIL
                     QAACVNPVRHYGLPLGLLQPGHTADFIIVDDLNTFKPREVWLRGRCVARDGVSFLPNG
                     EPEIANRFAASFVSVPDLSFPQPTGRRVRVIGVRDGELVTDLLQLDPLLNAGKVVCDP
                     ERDLLKLVVCNRYAPARPAVGLVRGFGLQRGALASSVAHDSHHLIAVGATDAALVTAL
                     NEVIHRQGGLAVADDNSRVLAAFSLPLAGLMSTDDAETVARSYSDCDRLAKLLGTRLT
                     APFMTLSFLALPVIPKLKLTDKGLFDGEALTHVPLFV"
BASE COUNT          656 a          931 c         1051 g          580 t
ORIGIN      
        1 ggattttccg cttttgctcg ctacacaaat tccgcttttg aaaactacac gacacgaatc
       61 gatacgctgt ttgacggacg ggctggaaat cgctatgata aaagaccata tggaaagcgg
      121 gttaccgtat tcgatcggta atgcgcttag cgagaggagt aagtatgatt cgcacaacac
      181 gtcccccgat ccgggtcggg cttgtcgggc tggggcgggc cggtttggag atgcatgtcc
      241 ccgaattgga gcaatacccg gaattgttca aaatcatcgc ggtctgcgat ccggtcaaag
      301 agcggcgcga tctggcggtg gaacgcatcc ccgagtgccg tacctaccgg cgttatgaag
      361 atctgctggc tgatcccgat gtggagctgg tggatatcgc cacacgatcc gacgagcatg
      421 ccgcgcatgg gatgcaggcc ctgaagactg acaagtgggt gaacatggag cgtccgttct
      481 gccgcgatta cgatgaggcg ctggtgttgc gcgccgcggc gatcaaggcg tcgaaccggc
      541 tcctcgtgcg ccacaactac cggtttgagc cggcgttcat gcaggtgcgc gaggtgatcg
      601 aatccggcgt gctcggcgaa ctctacgaca tcaagatccg acggggcgtg tacgaacgcc
      661 gtgacgattg gcagacggtg aagcgttgcg gcggcggggc ggccctcgcg tggggcacgg
      721 cgtttctcga tcaggcgctg gaattgttga ggacgccgcc gatcaaggtg tggtccgatt
      781 tcaagcgggt cgcttcggtc ggcgatgccg aggattatgt gcggatcatc ctgcgcaaca
      841 tggccggtct gaccgtggac ctcgaagtgt ccggcgggcg gatcggcgag atgccgcaat
      901 tcattgtgac cggcacaaaa ggggcgttca ccctttatcc cgatgccgaa caaggcacct
      961 tgcgctatct ggatcccaaa cagaagcttg cccggcgccg gtcgagcgtg cgcacgccgc
     1021 cgttaggcag tttcggcaca gcggagacgc tgcagtggtt ggagacgtcg gtacctgtca
     1081 agcccaaggc ggagtgcggc atgtcgctga tctggaagta tgtctaccag gccatccggg
     1141 agaacaagcc ctaccccgtg ccgctggacg tcgcgatcga aaccatgcgg atcctgtcgc
     1201 tggtcaagaa ggagagcccg ttcgcctgaa cggctttcgg ttcagacgaa cagcggcaca
     1261 tgcgtgaggg cttcgccgtc aaacaacccc ttgtcggtta gcttgagctt ggggatcacc
     1321 ggcagggcca gaaacgacag ggtcatgaac ggcgcggtca ggcgtgtgcc gagcaacttg
     1381 gcgagccggt cacagtccga ataggagcgc gccacggttt ccgcgtcgtc tgtggacatg
     1441 agtccggcca gaggcaggga gaaggcagcg agcactctgc tgttgtcatc ggccacggcc
     1501 agaccgccct gacggtggat cacctcgttg agggccgtga ccagcgcggc gtctgtggcc
     1561 cccacggcga tgagatgatg cgagtcgtgg gccacgctgg aggcgagcgc gccccgctgc
     1621 agaccaaacc cccggaccag gcccacggca ggacgcgccg gcgcgtaacg gttgcacacc
     1681 acaagcttga gcagatcgcg ctcaggatcg cagaccacct tgccggcgtt gagcaaagga
     1741 tccagttgca gaaggtcggt cacgagttca ccgtcgcgca caccgatcac gcgcacgcgc
     1801 cgaccggtcg gttgcgggaa actcaggtcc ggaaccgaaa cgaaggaggc ggcgaaacgg
     1861 tttgcgatct caggttcacc attcggcagg aacgacacgc cgtcgcgggc gacgcaacgt
     1921 ccccgcagcc agacttcgcg gggcttaaag gtgttcaggt catccacaat gatgaaatcg
     1981 gcggtgtggc ccggttgcag caagccgagc ggcaggccat agtgccggac cggattgacg
     2041 cacgccgcct gcaggatggc ctgaagcgga atgcctctcg tataggcctt ggccgcgatc
     2101 gtgttgatgt ggtcgtgaag cagatcatcg ggatgcttgt cgtcactgca aaacatgcag
     2161 tgttcgggat ggcgggtcag gagcggcaga aacggttcga aaaggtgcgt tgcggaaccg
     2221 tagcggatct gaagttggat accgccccgg atcttctccc gggcttcgtc aaaagagagg
     2281 ctctcgtggt ccgtggtgat gccggcgttg gcgtaagtcc gcatcgcctc gccggtgagc
     2341 cccggcgcat gcccgtcgat cggcaggcct cgggcccggg ccgcatccaa tttggcgcgc
     2401 acctcaggat cctcgttgag cacacccggc acattcatca tttccgagag atgggtgatg
     2461 cccggcagac cgagcaaccg cgtgacggca tcggaatcca gtgtcgcgcc cgcggtttca
     2521 aagggtgtgg ccggtacgca ggagggtacg ccgaaaccaa aaacaaaggg cgtctgtttc
     2581 gtcaggcgca gcatgagttc cactcccgcc tcacccagca cattggcgat ctcgtgcgga
     2641 tcgaccacag cggcaagggt gccgtgacgc actgcgaccc gcgcaaaggc ggctggggtg
     2701 agcatcgagc tttcaagatg cacgtgggcg tccaccagtc cgggcatgat gaaccccggg
     2761 tccggatcag gctcgggtgt gatggtgtcg atcacgcccg tgtcggagac ggtcaggcgc
     2821 gctccgtatg ccgtattctg gaccggatcc aaaagcgtgc cgcaaaatga gagagacata
     2881 agactcctta tccattcgag ccgcacctta acaaaaaccg cttgtcgcgg caatgccggc
     2941 gccacgggcg caccgattcc gacaggggta atctccggtt cgttcagggt taagccgcag
     3001 tgcgcccgtc cattaccatc aacagaaacg tctgcccgaa gtacgtttac ttcgttactc
     3061 cggcgtcaga agtggaatca cctgccgccg gttacggcgg tagacgtgga aatcgctgtc
     3121 aagggacata acccgatgct tcggaaaaag ctccgacatt ctgacgaggc aggcatccgc
     3181 cagcgacatg gggacatcgg catacttccg aaggaggt
//
LOCUS       DAOSRB010000275         3205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_27158_length_3205_cov_6.770794, whole genome shotgun sequence.
ACCESSION   DAOSRB010000275 DAOSRB010000000
VERSION     DAOSRB010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27158_length_3205_cov_6.770794"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLZ74_10675"
     CDS             <1..191
                     /locus_tag="PLZ74_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98822.1"
                     /translation="TRLTRPGASGELRFEGEAINAVRGVADGHNPSGLPPSITWTLEQ
                     NRLAFRLPIPKRLNPIGL"
     gene            complement(431..1426)
                     /locus_tag="PLZ74_10680"
     CDS             complement(431..1426)
                     /locus_tag="PLZ74_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98823.1"
                     /translation="MATNESNTLNGLIQINDLNFADINVTDLLQDAPVLQRMVAVPAS
                     QGGTLHKYIKQVVAAGASFRAVNTGVANAAPQQEDVEVVCKYLDGSFQRDIAIADGYR
                     GGRAAYMQKETAKALLALFAGLEKQILQGTASGISGTGFNGLADNSFVSNLHQSMVVN
                     AGGNGGRSVWLVRSTEDDVAIVAGNDGQLKFDFDPESVQFLVTDTKTGAGYNALSAAL
                     GGWFAVQYGSRYSLGRIANIDGTSGHALTDKLIAEALSKFPAGRQPNVIVMDRTSWHE
                     LQASRTATNPTGAPAPFPADAFSIPIVVSEHVKTDESALVTTTTTTTTTTTATTQ"
     gene            complement(1452..2822)
                     /locus_tag="PLZ74_10685"
     CDS             complement(1452..2822)
                     /locus_tag="PLZ74_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98824.1"
                     /translation="MWLTAWKRKRAKMNKWLSTGYLKSKPEGAVDRERGIIPGALVVT
                     EGEAKGHGVFLEAEFVDEVIRQGNEAKAGLKARFGHPNMCSTALGTFLGRWKNFRRDT
                     VTRDDGSQAVAARADLFLSQSAKDTPHGDLYEYVLGLAEKEGDMFGTSIVFTPGDGYR
                     KTKSGKKVVSVWERDEKGGFVFNRDGQRLFRWIDENGKDHDPAADPVIERDYAVCQKL
                     HACDAVDDPAANDGLFSRFSQETVAGQVTEFLDLHPEVFNAVADHPEILEALANYGNR
                     VDEFIQRYQEYKKQKNGETNMESNDTPGAETPETPPSAEPEGTVSTTETTEPETTPET
                     QPETPPAAAPESAPPEPPPDPETPPPPATEQQSDESRAEFKRMCADFGQEIAAQVFAD
                     GGGYDAAREAYYKAIEAENKALKAKVAALPSGGTPAAFVVGDKPAEVDENGVHRLFRQ
                     GTRRNR"
     gene            complement(2779..>3205)
                     /locus_tag="PLZ74_10690"
     CDS             complement(2779..>3205)
                     /locus_tag="PLZ74_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98825.1"
                     /translation="CRSKREKNSWKRREYSDWVLETVWNDPKLSAAWNLKQIANDAGF
                     SRLRELQEEVEWVPSGFPWLMKGQEIEGDTKAIAIGADNIIDSCTRRGSNFFENIDKQ
                     AEAEAYAKEKGVALVKGLPGQAVAQDVVDGVEEKEGEDE"
BASE COUNT          568 a          973 c          923 g          741 t
ORIGIN      
        1 aaactcgatt gacgcggcca ggggcatcag gcgaactccg cttcgagggt gaggctataa
       61 atgccgtccg tggggtcgct gacggccaca atccgagcgg gcttccaccc agcatcacct
      121 ggacgctgga acaaaatcgc ctggccttcc ggcttcccat tcccaaacgc ctcaacccaa
      181 tcggcctcta gtaccttgat caacgacgca accgccatca actgaccctc ttccgtgttc
      241 gttcgcgttg tatcgaaacg accgcatagg catctgtcca gttgcttgcg accgattcgg
      301 atgcttgccc gcatggttgg ctgcgtggca aacatcgcct cgaaggatct ggagaatatg
      361 cccattggtg agggggccat gggccgcagc cgccaggagg tagcgacggc ggccccttgg
      421 ctgtggctga ttattgcgtg gtggcggtcg tggtggtcgt ggtggtcgtg gtggtcgtca
      481 ccagcgcgga ttcgtccgtc ttgacgtgtt cagacacgac aatcggaatg ctgaacgcat
      541 cggccgggaa cggggcgggc gcaccggtcg ggttcgtcgc cgtgcggctc gcctgcaact
      601 cgtgccagct cgtacggtcc ataacaatga cgttgggctg acggccagcc gggaacttgc
      661 tcaaggcttc ggcgatgagc ttgtccgtca gcgcatgtcc gctggtcccg tcgatgttgg
      721 cgatgcggcc gaggctgtac cggctgccgt actgcacggc aaaccagccg ccaagcgcgg
      781 cagacagggc attgtagccc gcgccggtct tcgtgtccgt aaccaggaac tgcacggact
      841 cggggtcgaa gtcgaacttc aactgaccgt cgttgccagc cacaatggcc acgtcgtcct
      901 cggtggagcg aacaagccaa acagatcggc ctccgtttcc gcccgcgttc acaaccatgc
      961 tctggtgcag attggaaaca aacgagttgt ctgcaagccc gttgaagccg gttccgctga
     1021 ttccggacgc cgtgccctga aggatctgct tctcgaggcc ggcaaacagg gccagcaaag
     1081 ccttggcggt ttctttctgc atgtacgccg cacgcccgcc gcggtaaccg tcggcaatgg
     1141 cgatgtcgcg ctggaagctg ccgtcgaggt acttacagac gacctcaacg tcttcctgct
     1201 gcggagccgc gttcgcaacg ccggtattga cggcgcggaa tgaagcgccg gccgcgacaa
     1261 cttgcttgat gtacttgtgg agcgtgccgc cttgcgaggc gggaacggcc accatccgct
     1321 gcaaaacagg ggcgtcttgc agaaggtcgg tgacgttgat gtcggcgaaa ttcagatcgt
     1381 tgatctggat caacccatta agggtattgg attcgtttgt agccatggtt tttctttctt
     1441 attcgtttat gttatcggtt tcgccgcgtc ccttggcgga acaggcgatg aacgccattc
     1501 tcgtcaactt ctgcgggctt gtcccctacg acaaaagccg caggagtccc gccactcggc
     1561 agggccgcaa ctttagcctt cagggctttg ttctccgctt cgatggcctt gtaataggct
     1621 tcacgggcgg catcataccc gccaccgtct gcgaacacct gcgcggcgat ctcctggccg
     1681 aagtcggcgc acatgcgctt gaactcggcg cggctttcgt cgctctgctg ttcggttgcc
     1741 gggggcggtg gagtttccgg atcgggcggt ggctcgggcg gtgcgctctc cggcgcggcg
     1801 gcaggaggtg tctccggctg cgtttccggc gtcgtctctg gttccgttgt ctccgttgtg
     1861 gaaacagtac cttccggctc tgcggagggc ggcgtctccg gcgtctccgc gccgggcgtg
     1921 tcgtttgatt ccatgttcgt ttctccgttc ttttgtttct tatattcctg ataccgctgg
     1981 ataaactcat ccacgcggtt gccgtagttc gccaatgcct caaggatttc ggggtgatct
     2041 gcaacggcgt tgaaaacttc ggggtgaaga tcaagaaact ccgtcacctg cccggcaacg
     2101 gtttcctgcg aaaagcggct gaacaggccg tcatttgcgg ccggatcatc aaccgcatcg
     2161 caagcgtgga gtttttggca aacggcgtaa tcccgttcaa tgacaggatc ggcggccggg
     2221 tcgtgatcct ttccgttctc atcgatccac ctgaacaatc gctggccgtc gcggttgaat
     2281 acgaaaccgc ctttttcgtc acgctcccaa acgctgacaa ccttcttgcc agatttcgtt
     2341 ttgcggtatc catcgccggg tgtgaacaca attgacgtgc cgaacatgtc gccctctttt
     2401 tcggccagcc caagaacgta ctcatacagg tcgccgtgcg gggtatcctt tgccgactga
     2461 ctcaaaaaca ggtcggctct cgcggcgaca gcctgcgatc catcgtcgcg ggtaacggtg
     2521 tctctgcgga aattcttcca gcgtccaagg aaggtaccga gagcggtgct gcacatgttc
     2581 ggatgtccga accgggcttt aagtccggcc ttcgcttcat tgccctggcg tataacttca
     2641 tcaacaaatt cggcctcgag gaacactcca tgaccctttg cctccccttc cgtgacgacc
     2701 aacgcgccgg gaatgattcc acgctcgcgg tcaactgctc cttcaggctt cgatttaaga
     2761 tacccggttg aaagccactt attcatcttc gccctctttc tcttccacgc cgtcaaccac
     2821 atcttgcgcg acggcttgcc ccggcaaacc cttgaccagg gcgacgccct tttccttggc
     2881 atacgcttcg gcctcggcct gcttgtctat gttctcaaag aagtttgagc cgcggcgggt
     2941 acaggagtcg ataatgttgt ccgcgccgat ggcgattgcc tttgtgtcac cctcgatttc
     3001 ctgccccttc atcaaccaag gaaagccgct aggaacccat tctacttctt cttgtagctc
     3061 gcggagcctg gagaatccgg catcgttggc gatctgtttc aggttccagg cggcggaaag
     3121 ttttggatca ttccagacgg tttcaagcac ccaatcgctg tactccctgc gcttccaaga
     3181 gttcttctcg cgcttggatc ggcaa
//
LOCUS       DAOSRB010000276         3184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_27422_length_3184_cov_5.989773, whole genome shotgun sequence.
ACCESSION   DAOSRB010000276 DAOSRB010000000
VERSION     DAOSRB010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27422_length_3184_cov_5.989773"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLZ74_10695"
     CDS             <1..297
                     /locus_tag="PLZ74_10695"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="catalyzes the formation of pyruvate and glyoxylate
                     from 4-hydroxy-2-oxoglutarate; or pyruvate and
                     D-glyceraldehyde 3-phosphate from
                     2-dehydro-3-deoxy-D-glyconate 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="keto-deoxy-phosphogluconate aldolase"
                     /protein_id="HOR98826.1"
                     /translation="IEAGLDLGCRLLKFFPAEALGGVKLLTALSAPYRHTGVRFMPTG
                     GVTTANLADYLGLPAVAAVGGTWIAKKEEMAAGAWEAVAQRCRDARAVVRQVRG"
     gene            complement(337..2124)
                     /gene="yidC"
                     /locus_tag="PLZ74_10700"
     CDS             complement(337..2124)
                     /gene="yidC"
                     /locus_tag="PLZ74_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOR98827.1"
                     /translation="MNKQERIIAFLLGLALVGWMVHSHRMAAKQAREAAERPVATETA
                     APAAPAAPAQAALTAPVATQPQPALPSEPEEVATLRGDRMTVEVSSHGAVLKRVTLDG
                     YYEKPGKPGPDNPPVTFDFAGAPALELSGVPGLADRAAYRVQRHEQECAVTLTAVTPQ
                     GLSVTRRMELSNGYKIRITDQINNTGDSGITLGTNSVQLGAIGRGTSKNELLSIDSLP
                     ASAGAKTRHWGSEKATKSLLAAGASGGMGCGRAPSAVGLADHISVPIHEPQAWIALKS
                     RFFVTLFSASETNCGFVAGMARDTALPTYALKRLSARALFAGRTLAPGESLTRDYTLY
                     VGPKKLSRLQAMGNRMDEIMEFGTFTWFCKLLVPTLNFFHRLIPNYGIAIILLTFLVR
                     ILFWPLTHKSTQSMRKMQEIQPKLKEIQAQFKDNPQKMQQETWAVYRENKVNPLSSCL
                     PMLIQIPVFIALFTVLRSAVELRYASFLWITDLSEPENLFADVLPLPLNILPFLMSGT
                     MALQSYLTPSMGDPQQQKMMMFMMPIMMLVMFYGFPSALSLYWTVSQVLSIIQMLMMR
                     RKGHTPHDGDMTVEPPLTRQQRRQAARAH"
     gene            complement(2121..2411)
                     /gene="yidD"
                     /locus_tag="PLZ74_10705"
     CDS             complement(2121..2411)
                     /gene="yidD"
                     /locus_tag="PLZ74_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOR98828.1"
                     /translation="MGSTATFISRTIRSMLIALVRAYQVVLSPHFGSCCRFEPSCSAY
                     CIEALRTHGVLRGGWLTLKRIVRCRPFGPSGYDPVPSGKQDAVQPEMTGKNV"
     gene            complement(2423..2845)
                     /gene="rnpA"
                     /locus_tag="PLZ74_10710"
     CDS             complement(2423..2845)
                     /gene="rnpA"
                     /locus_tag="PLZ74_10710"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOR98829.1"
                     /translation="MSAGDSHDPGRIGNGRRPRLRLFRLDAPAYKRVFAEKRSVYGRS
                     LVMWIAQGADADRRVGVIVSKRNFRRAVDRARAKRLLREAFRLSRPFLAPDADLILIA
                     RTGIGGQTCREVMADFERVCRRARIWRRDADPENKTHA"
     gene            complement(2850..2987)
                     /gene="rpmH"
                     /locus_tag="PLZ74_10715"
     CDS             complement(2850..2987)
                     /gene="rpmH"
                     /locus_tag="PLZ74_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOR98830.1"
                     /translation="MKMMWQPSKIKRARKIGFRARMATRNGRAIINARRRKGRIRLTQ
                     V"
BASE COUNT          567 a          968 c         1049 g          600 t
ORIGIN      
        1 atcgaggccg gactggatct gggctgccgt ctgctgaaat ttttcccggc cgaggcgctg
       61 ggcggggtga agctgctcac ggccctctcc gcaccctaca ggcacaccgg cgtacgcttc
      121 atgccgaccg gcggggtgac gaccgcgaac ttggcggatt atctgggtct gcccgccgtg
      181 gctgcggtgg gcggcacgtg gatcgccaag aaagaggaga tggcggccgg cgcgtgggag
      241 gcggtggcgc aacgttgccg cgacgcccgc gcggttgtcc ggcaggtgcg cggatagggg
      301 gggggaagcg gccgtccgtc gtattccgcc cgttcctcaa tgcgcgcggg ccgcctgacg
      361 gcgttgctgg cgcgtcagcg gcggctctac cgtcatgtcg ccgtcatgcg gcgtatggcc
      421 cttgcgccgc atcatcagca tttggatgat ggagagcacc tggcttacgg tccagtagag
      481 cgagagcgcc gaggggaagc cgtagaacat gacgagcatc atgatcggca tcatgaacat
      541 catcatcttc tgctgttgcg gatcgcccat cgagggcgtc aggtagcttt gcagcgccat
      601 ggtgccggac atcaagaacg gcaggatgtt cagcggcagg ggcagcacgt ctgcgaacag
      661 gttttccggt tcactcaggt cggtgatcca gaggaacgac gcgtagcgca gctcgactgc
      721 gctgcgcaac acggtgaaga gcgcgatgaa caccgggatc tggatcagca tcggcaggca
      781 actggagagc gggttgacct tgttctcgcg gtaaaccgcc caggtctcct gctgcatctt
      841 ctgcgggttg tccttgaact gcgcctggat ctctttcagc ttcggctgga tctcctgcat
      901 cttcctcatg ctctgggtgc tcttgtgcgt aagcggccag aagaggatcc gcaccagaaa
      961 ggtgagcagg atgatggcga taccgtaatt ggggatcagg cggtggaaga agttgagcgt
     1021 gggcaccagc aacttgcaga accaggtgaa ggtgccgaac tccatgatct catccatgcg
     1081 gttgcccatg gcttgcaatc gcgagagttt cttgggaccg acatagagcg tatagtcgcg
     1141 ggtcaggctc tcgccgggcg ccagcgtgcg cccggcgaac aacgcgcggg cggagagccg
     1201 cttcagggcg taggtcggca gggcggtatc gcgggccatg ccggccacaa agccgcagtt
     1261 ggtttcagag gcggagaaga gggtcacgaa aaaacgggac ttgagcgcga tccaggcctg
     1321 cggctcgtgg atcggaacgc tgatgtgatc ggcgaggccc acggcagagg gcgcgcgccc
     1381 gcaacccatg ccgcccgacg cgccagccgc cagaagggac ttggttgcct tctcggaacc
     1441 ccagtgacgc gttttcgccc cggccgaagc gggtagcgaa tcgatcgaga gcagttcgtt
     1501 cttggacgtc cccctgccga tcgcgccgag ctgcacgctg ttggtgccca gcgtgatccc
     1561 gctgtcgccc gtattgttga tttggtcggt gatgcggatc ttgtagccgt tggaaagctc
     1621 catgcggcgc gtcacgctca acccctgcgg cgtgacggcg gtcagggtca ccgcgcattc
     1681 ctgctcatgg cgctgcacgc ggtacgccgc gcggtccgcc agacccggca cgccggaaag
     1741 ttccagcgcc ggggcaccgg cgaaatcaaa ggtgaccggc ggattgtccg gaccgggttt
     1801 gcccggtttc tcataatacc cgtccagcgt gacccgtttc aggaccgcgc cgtgcgagga
     1861 gacctctacg gtcatgcggt cgccgcggag ggttgcgacc tcctccggct cggaagggag
     1921 ggcgggttgc ggttgcgtcg ccacgggggc agtcagcgcc gcctgcgccg gggcggccgg
     1981 tgccgcaggg gccgcggttt ccgtcgcaac ggggcgttcg gcagcctccc gcgcttgttt
     2041 cgcggccatg cggtggctgt gcaccatcca gccgaccaag gccaatccta ataaaaatgc
     2101 gatgatccgt tcttgcttat tcatacattc tttccggtca tttcaggttg gacggcgtct
     2161 tgttttcctg acggaaccgg gtcatacccg gagggtccga acggacgaca gcggacgatc
     2221 cgtttgagcg ttagccagcc gccccggagc acgccgtggg tccgcagcgc ctcgatacag
     2281 taagcggaac aggacggttc gaaacggcaa cacgatccga agtgcggcga aagcacgact
     2341 tggtacgcgc gcaccagcgc gatcagcata ctgcggatcg tccggctgat gaatgtggcc
     2401 gtgctgccca tccgttgatt cctcacgcat gtgttttgtt ctcagggtcc gcgtcgcgcc
     2461 gccagatccg ggcgcggcgg cagacacgtt cgaaatccgc catcacttcc cggcaggtct
     2521 ggccgccgat gccggtccgg gcgatcagaa tcagatccgc atccggtgcc agaaaagggc
     2581 ggctcaggcg gaacgcctcg cgcagcaacc gtttggcgcg ggcgcggtcc acagcccgcc
     2641 ggaaattccg tttggaaaca ataacgccga ccctccggtc ggcgtctgcg ccctgtgcaa
     2701 tccacatcac cagcgaccgg ccgtaaaccg acctcttttc cgcgaaaacc cttttatagg
     2761 cgggcgcatc aagccggaaa agacgcaacc tgggcctccg gccgtttccg atccggcccg
     2821 ggtcatggct gtcgcctgcg gacatggctt tacacctggg tcaggcggat ccgcccttta
     2881 cggcggcggg cattgatgat cgcgcgcccg ttgcgggtgg ccatccgggc ccggaatccg
     2941 attttacggg cccgtttgat cttcgagggc tgccacatca ttttcatggt ttcggtatcc
     3001 tccttcgtct gagtcaaaaa aagagttaac actttaacat agtccccggt agcgggtcaa
     3061 ccgattgacc gtttttggac gtttcccgcg gtatgccata gtcatggaca ctgtagccaa
     3121 aatcgcggtg tccagaaatc aagcgcatcc gtcaaatact ggacaccgtg cgcgaagata
     3181 tcgc
//
LOCUS       DAOSRB010000277         3176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_27505_length_3176_cov_4.900352, whole genome shotgun sequence.
ACCESSION   DAOSRB010000277 DAOSRB010000000
VERSION     DAOSRB010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27505_length_3176_cov_4.900352"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1824)
                     /locus_tag="PLZ74_10720"
     CDS             complement(103..1824)
                     /locus_tag="PLZ74_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOR98831.1"
                     /translation="MLRQSARGIGFVALVFSLTVAALLTADATRSGSAETVRAETLQR
                     TLAASRTTPTDPDAVAFARELDRLARRAYFNSLTFRQTGMLLLAVGLILTAAGFGVAW
                     RLTLLIPDPRTLGESDPARTDRMAVNAVLATGGVLLLCAVVLHVTQRRRTAAQSADRD
                     LRARLQNPAAPPDATRVCPCRQGTDPAALTEHWPFLRGPEMSGRSPLPRFPVRWNGVT
                     GESVVWKTELRHGGSSTPVVWSNLLFLTSGNAEARSVMAHDTKTGSLLWHTEIPDGDK
                     TGAALPDVMDETGLAASTPACDERHVYAVFGTGDLVALDHTGKPVWQIFLGRPKNTYG
                     HASSLVYCGRMLIVQWDQEEHGRILALDKSTGRTLWETPRSAGMSWSTPVIMPVCDAF
                     LLLVHATQQTWGMDLATGAKRWQVEAVGGEVAPSLTWEGDVWLAANCYSKMVAFRLPP
                     QGPPEKLWEWEDGNLPDVASPVILDGLVYLANDAGEVFCHDLTNGKQVWLKEFEDGFY
                     ASPVAAGGHVYVVDRVKGVFRVFAAGREGRNVSTNPMGEAVSATPAFARGHIFVRGRK
                     HLWAIGM"
     gene            1995..>3176
                     /locus_tag="PLZ74_10725"
     CDS             1995..>3176
                     /locus_tag="PLZ74_10725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR98832.1"
                     /translation="MSIRNEVPTQEDIAKIDPLALSHMRPDWSASQPITLAQKRWLFA
                     VVAGVAVAALVWPLRTAQVLIAVCTALYVVIMLYKFIVIRASLSPSALLRFSPQDLVA
                     CEPREWPVFSVLVPMYKEPETVKQMVASLEALDYPADKKDVQILLEADDDATCEAARA
                     LSMPQGFRLTVVPPSFPRTKPKACNVGLALARGKYLVIYDAEDLPERDQLKKAVLAFE
                     SSPDNVVCIQSRLNYYNPRQNTLTRWFTAEYSAWFDLQLPGLAALGAVIPLGGTSNHF
                     RTEVLRELLGWDAYNVTEDCDLGVRLARAGYATRMLETTTWEEACSRPAFWIRQRTRW
                     QKGYIQTWFVHMRRPLALLRELGVVNFLHYQLLIGGVTFSVLVNPVFWMMTLVWFVFR
                     PE"
BASE COUNT          567 a          959 c         1032 g          618 t
ORIGIN      
        1 gcctgcggga gagtaggacg ccgccgggag tttttttccg gggggccgcg gatcgtcccg
       61 atccgcggcc cccccttttt ttgcccgagc tttcggacta cattacatcc cgatggccca
      121 gaggtgcttc cgaccccgta cgaaaatgtg tccgcgggca aaggccgggg tggcgctgac
      181 agcctcgccc atggggttgg tcgaaacatt ccggccttcg cggcccgccg caaagacgcg
      241 gaaaacacct ttgacgcgat ccaccacata cacatgaccg cctgcggcaa ccggtgaagc
      301 gtagaagccg tcctcaaact ctttgagcca gacctgtttc ccgttggtca gatcgtggca
      361 gaatacctcg cccgcatcat tggccagata caccagaccg tcgaggatga ccggactggc
      421 cacatcgggc aggttgccgt cctcccactc ccagagtttt tccggcggac cctgcggcgg
      481 aagacggaag gcgaccatct ttgaataaca gttcgcggcc agccagacat caccctccca
      541 tgttagcgag ggcgccacct cgcccccgac cgcctcgacc tgccaccgct tcgcgccggt
      601 cgccaaatcc attccccagg tctgctgcgt cgcatgcacc aacagaagga acgcgtcaca
      661 gaccggcatg atcacgggcg tgctccagga catgcccgct gagcgcgggg tctcccatag
      721 agtacgcccg gtcgatttgt ccaacgccaa gatgcggccg tgctcctctt ggtcccactg
      781 cacaatcagc atgcgtccgc aatagacgag cgaggaggca tgtccatagg tgtttttcgg
      841 acgacccaga aaaatctgcc acaccggttt gccggtgtga tccagcgcca cgaggtcccc
      901 ggtgccgaac accgcgtaca catggcgctc atcgcaggcc ggggtcgatg cggccaagcc
      961 ggtctcatcc atcacatccg gcagtgctgc gcccgttttg tctccatccg gaatctcggt
     1021 gtgccacagc agggatccgg ttttcgtatc atgcgccatc acactgcgtg cctcggcatt
     1081 tccggaagtc agaaaaagga ggttgctcca gaccaccggt gtgctggagc cgccgtgtcg
     1141 taactccgtc ttccagacga cgctttcgcc ggtgacgccg ttccagcgta ccgggaagcg
     1201 ggggaggggg gagcggcccg acatctccgg accccgcaga aagggccagt gctctgtaag
     1261 cgcggccgga tcggtgcctt ggcgacaggg acagacgcgc gtcgcgtcgg ggggggctgc
     1321 cggattttgc aggcgggcgc gcaggtcacg gtcagcggat tgggcggcag tgcggcggcg
     1381 ctgggtcaca tgcagcacaa cggcgcaaag cagtaatacg ccaccggtcg ccagaaccgc
     1441 gttgactgcc atgcggtctg ttcgggcggg atcggattcc cctaatgtgc gcgggtccgg
     1501 gatcagcagg gtcagacgcc aggcgacacc gaaaccggcc gcagtgagga ttaagccgac
     1561 ggccaagaga agcatgcccg tctgacggaa ggtcaggctg ttgaaatagg cgcgtcgcgc
     1621 gagacgatcc aactcgcgcg cgaacgctac ggcatccgga tccgtcgggg tggtgcggct
     1681 cgccgccagt gtccgttgca gcgtttcggc acgcacggtc tccgctgaac cggagcgggt
     1741 agcgtccgcg gtcagcaggg ccgcgaccgt aagactgaag accagtgcca cgaacccgat
     1801 accgcgcgca ctttgccgca gcatccggtt ccgtgcttcc atgcctgtgc acctcatgcc
     1861 gagttctccc ttcgcggttc caactttatt cggtatctta gcggaggcgg aaaggtgtgg
     1921 caacactctt cctcttgtga gggacgtgtg gtacagtgat acggggatgg ggtttggggg
     1981 agggatatcg aagcatgagc atcaggaacg aagtgccgac gcaggaagac atcgccaaga
     2041 ttgacccgct ggcgctgtcg cacatgcggc cggattggag tgcgtcgcag ccgataacgc
     2101 ttgcgcagaa gcgctggctg ttcgcggtgg ttgcgggtgt ggctgttgcg gctttggtgt
     2161 ggccgttgcg gacggcacag gtgctgatcg cggtctgtac cgcgctgtat gtcgtgatca
     2221 tgctgtacaa attcattgtg atccgcgcgt cgctgtcgcc ctctgcgctg ttgcgcttct
     2281 cgccgcagga tctggtcgcg tgcgagcctc gggaatggcc ggttttctcc gtgctggtgc
     2341 ccatgtacaa agagccggag acagtcaagc agatggtggc gtcgctggag gctttggatt
     2401 atccggcgga caagaaggac gtgcagattc tgctggaggc ggacgatgac gccacgtgcg
     2461 aggccgcgcg tgccctctcc atgccccaag ggttccggct gacggtcgtg ccgccgtctt
     2521 ttccgcggac caagcccaaa gcgtgcaatg tgggcttggc gttggcccgg ggcaaatatc
     2581 tcgtgattta tgatgcggag gacctcccgg aacgggacca gctcaaaaaa gcggttctgg
     2641 ccttcgagtc ctcgccggat aatgtggtct gtatccaatc gcgcctcaat tattacaacc
     2701 cccgtcagaa tacgctgacg cgctggttca cggccgagta ctccgcgtgg ttcgaccttc
     2761 aacttccggg gttggcggca ctcggcgcgg tcattccgtt gggcggcacc tccaatcatt
     2821 ttcggacgga ggtgctgcgg gagttgctgg gatgggacgc ctacaatgtc acggaggatt
     2881 gcgatctggg ggttcggctg gcacgcgcgg gctacgccac acgtatgctg gaaaccacca
     2941 catgggagga ggcgtgcagc cgccccgcgt tctggatccg gcaacgtacc cgctggcaga
     3001 aaggttacat ccagacgtgg ttcgtacaca tgcggcggcc gctcgcgctg ctgcgtgaac
     3061 tgggggtggt gaatttcctg cattaccagt tgctgattgg gggggtgacc ttctcggtgc
     3121 tggtgaatcc ggtgttttgg atgatgacgc tggtctggtt tgtgtttcgc ccggaa
//
LOCUS       DAOSRB010000278         3151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_27795_length_3151_cov_7.110788, whole genome shotgun sequence.
ACCESSION   DAOSRB010000278 DAOSRB010000000
VERSION     DAOSRB010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27795_length_3151_cov_7.110788"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /gene="hflX"
                     /locus_tag="PLZ74_10730"
     CDS             complement(<1..807)
                     /gene="hflX"
                     /locus_tag="PLZ74_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOR98833.1"
                     /translation="MKGYDTHRVERALIAQLVLAGTDPAEAADSLLELGRLAETAGLE
                     VVGQITQNRIKPDGGTLFGSGKVQDLKTLLAETAANTVVFDNPLSAIQAYQLARELDA
                     RIMDRTELILEIFARRARSAEAQLQVELAKTQFLLSRIPVTEAQQRFAGGGGGAYVRG
                     PGESHLSLRNAPLRRRVTELRKRLATIQKRQTVRDRAKREWPVVCLVGYTNAGKSTLL
                     NVLADAGAYVDDRLFATLDTKTRKVWLAPSREILLTDTVGFIRNLPHGLIA"
     gene            complement(804..1319)
                     /gene="lptE"
                     /locus_tag="PLZ74_10735"
     CDS             complement(804..1319)
                     /gene="lptE"
                     /locus_tag="PLZ74_10735"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOR98834.1"
                     /translation="MRLTVSLTAATLLLAAGCASYTCTSAVPEELRTLAVPVFENESG
                     FPEIDAVVTQQVLREFQREGTFRIARQDAASLKLLGKLVKTDMQSLNYDRNYGSRTSE
                     YRYGLVAEITLVERGTGKLLIDAQRVKANTTFLTHGDMLTGVQDAYARAARELARAIV
                     DTVLAHWPSPQ"
     gene            complement(1336..2367)
                     /gene="bamD"
                     /locus_tag="PLZ74_10740"
     CDS             complement(1336..2367)
                     /gene="bamD"
                     /locus_tag="PLZ74_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOR98835.1"
                     /translation="MMRQGFIRIIAAAALAAAWCSSFAQQTGRSGFHRGYATDSFEGF
                     EALDKDDRIPQKKKSFWYGVREETAAAQLDYALRQEAAGRDRTARKAYEALIREWPTA
                     PQAAEAQLALAALFERTGKFERAFDEYQYLLTYFSGHCPYDEVLDRQFRIANHLLHNN
                     RSMFGWMLSGTDSIRERFEKIVRNAPRSAIAPEVMLIIGGIRVSERELPEAIAVYDGI
                     LNRFPASPQAMHAAYLAAQCRYDLAMKHTYNEPRCREAIAFSKALLARLPNHPQKEQL
                     TVWLNALTEQLIDQNYQRAVFYDTRQRNATAAQAAYRRFLSEFADSKYAEQVRARLVE
                     LDRDAAPAP"
     gene            2551..>3151
                     /locus_tag="PLZ74_10745"
     CDS             2551..>3151
                     /locus_tag="PLZ74_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR98836.1"
                     /translation="MKKVLIPTKLDVVAAELLKAHGGYAVVQDAKTPLEELVKEHADA
                     YALIVRSEKVTAAIIDALPQLKVVVRAGAGYDTIDIKHARKKGVDVMNTPGANANAVA
                     EEVIAMMLADARHIVQADTTTRAGGWEKKNYMGREISGKTVGIVGLGNIGRLVAKRLR
                     GFECRILGYDPLVNRDRLRDLGIESATLEEIFSQSDYVTL"
BASE COUNT          547 a          931 c         1081 g          592 t
ORIGIN      
        1 ggcgatcagc ccatgcggca ggttacggat gaagccgacg gtgtcggtca gcagaatttc
       61 gcggctgggg gcgagccaga ctttgcgcgt cttggtgtcg agcgtggcga aaaggcggtc
      121 gtccacataa gcgcccgcgt ccgcgagcac gttgaggagc gttgacttgc cggcgttggt
      181 gtagcccacc agacagacaa caggccactc gcgcttggcg cggtcacgca cggtctggcg
      241 tttctggatc gttgccagac gtttccgtaa ctcagtgaca cgccgccgca gcggcgcgtt
      301 gcgcaggctg aggtggcttt cgccgggacc gcgtacgtag gcaccgccgc caccgccagc
      361 gaagcgctgc tgcgcttcgg tgaccgggat acgcgagagc aggaactggg tcttggccag
      421 ttcgacttga agttgcgctt cggcggaacg cgcgcggcgc gcgaaaattt cgaggatcag
      481 ttccgtgcgg tccataatgc gcgcgtcgag ttcgcgcgcg agttggtacg cctggatcgc
      541 gctgagcggg ttgtcgaaca ccaccgtgtt cgcggcggtc tccgccagca gggtcttcag
      601 atcctgcact ttgccggagc cgaacagggt gccgccatct ggtttgatcc ggttttgggt
      661 gatctgtcct acgacctcca gaccggcggt ctcggccagg cgccccaact ccagtagcga
      721 atcggcggcc tcggccggat cggtgccggc caacacgagt tgggcaatca gggcacgttc
      781 gacacggtgt gtgtcgtacc ccttcattgc ggagagggcc agtgggcgag cacggtgtcg
      841 acaatcgcac gcgccagttc gcgtgccgcg cgcgcgtagg cgtcctgcac gccggtcagc
      901 atgtcgccat gggtcaggaa ggtggtgttg gccttgacgc gctgggcgtc gatcagcagt
      961 tttccggtgc cgcgttcgac cagcgtgatt tccgccacca gaccgtaacg gtattcggag
     1021 gtgcgggaac cgtaattgcg gtcatagttc agcgactgca tatcggtttt gaccagcttg
     1081 ccgaggagtt tcagcgaggc cgcgtcctgg cgggcgatgc ggaacgtgcc ctcgcgctgg
     1141 aactcgcgca gcacctgctg cgtgaccacg gcatcgattt cggggaaccc cgactcgttc
     1201 tcaaaaaccg gaacggccag ggtgcgcagt tcttccggca cggcggaagt gcaggtgtag
     1261 gaggcgcagc cggcggccag cagcagtgtc gcggcggtca aggaaacggt cagacgcatg
     1321 ggactctcct ttcattcatg gggcaggggc ggcatcccgg tcgagttcga cgaggcgtgc
     1381 gcggacctgc tcggcgtatt tggaatcggc gaattcggac aaaaagcgcc ggtaggcggc
     1441 ttgcgcggcc gtggcattac gctggcgggt gtcgtaaaag acggcgcgct ggtaattctg
     1501 gtcgatgagc tgttcggtca gggcgttcag ccagaccgtc aactgctctt tctgcgggtg
     1561 gttcggcagg cgggcgagca gggccttgga gaaggcgatg gcttcgcggc agcgcggttc
     1621 gttgtaggtg tgtttcatcg ccaagtcgta ccggcactgc gccgcgaggt aggcggcgtg
     1681 catcgcctgc ggggaggcgg ggaagcggtt caggatcccg tcatatacgg cgatggcctc
     1741 gggaagttcc cgctccgaga cccggatccc gccgatgatc aacattactt ccggagcgat
     1801 ggccgaacgc ggcgcgttcc gaacgatctt ctcgaagcgt tcccggatgc tgtccgtgcc
     1861 gctgagcatc cagccgaaca tgctccggtt gttgtggagc agatggttgg cgatgcggaa
     1921 ctggcggtcc agcacttcgt cgtacgggca gtgcccggag aagtaggtga gcaggtactg
     1981 gtattcgtcg aaggcccgct cgaacttccc ggtgcgctcg aacagggcgg cgagggccag
     2041 ttgggcttct gcggcctggg gggccgtggg ccactcgcgg atcagcgcct cataggcttt
     2101 acgcgcggtg cggtcccggc cagccgcctc ttgccgcaat gcgtaatcga gttgcgcggc
     2161 ggctgtttcc tcgcggaccc cgtaccagaa cgactttttt ttctggggga tgcggtcgtc
     2221 tttatccaaa gcctcaaatc cctcgaacga atcggtcgcg tatccccggt ggaagccgct
     2281 gcgtccggtc tgctgcgcga aagaagaaca ccaggctgcc gccagggcgg cggcggcgat
     2341 tatccgtatg aacccctgcc tcatcatctg tttttccgat ctgcgagttt cggtctgccg
     2401 cgttgccgtc cggcgtaacg gggcttgatt cccgttccgc acaagattac agtattaccc
     2461 tgtttcggac ggtcggcgaa tgggtcgcgg ccgaccggga ctgccaagcc atgtacgtta
     2521 acgtagcaaa ccccggggaa ctgtagaagc atgaaaaaag ttttgatacc caccaagtta
     2581 gatgtcgtgg ccgccgagct tctgaaagcg catggcggtt atgcggtcgt gcaggatgcc
     2641 aaaacgccgc tggaggagct ggtcaaggag cacgcggacg cctacgcgct gatcgtgcgc
     2701 agcgagaaag tgaccgccgc gatcatcgac gcgctgccgc agctcaaggt ggttgtgcgc
     2761 gccggcgcgg gatatgacac gatcgacatc aagcacgccc gcaaaaaagg cgtggacgtg
     2821 atgaacacgc cgggggcgaa cgccaacgcc gtggcggagg aggtcatcgc catgatgctg
     2881 gccgatgcgc gccacatcgt gcaggccgat accacgacgc gcgccggcgg ctgggagaag
     2941 aaaaactaca tgggccgcga gatctccggc aagaccgtgg gcattgtcgg tctcggcaac
     3001 atcggccgtc tggtcgccaa acgcctccgg ggcttcgagt gccgcatcct cggttacgat
     3061 ccgctggtca accgcgaccg cctgcgcgac ctcgggatcg agtccgccac gctggaggag
     3121 attttcagcc agagcgatta cgtcacgctc c
//
LOCUS       DAOSRB010000279         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_28314_length_3106_cov_6.427401, whole genome shotgun sequence.
ACCESSION   DAOSRB010000279 DAOSRB010000000
VERSION     DAOSRB010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28314_length_3106_cov_6.427401"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLZ74_10750"
     CDS             <1..210
                     /locus_tag="PLZ74_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98837.1"
                     /translation="PRRCHPPHALLPLQLPAVEGGGRQVLQSAASLDPTNPPDLTDPP
                     LRAGTPALPTPDAGFLRKRIWLGGA"
     gene            252..1532
                     /locus_tag="PLZ74_10755"
     CDS             252..1532
                     /locus_tag="PLZ74_10755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR98838.1"
                     /translation="MSETRNHAEHTAPASYKWLLVALLWVVFFLNQGNRQIYSTVLPQ
                     IRAATAQGGLGLSDVQAGLVATVFIAVYGVCVPLAGFLGDILRRKKQILFSLILFSAG
                     TLLTGFSGGMPGMILFYGILFGGGEALYYPPATSLLGQFHENSRATAFSIHQTALYLG
                     IMFSGYFSGYLARTMAGGWRAPFVVFGLAGIVWSGVVLFLMRDTPSPTRDAAAARVPL
                     RETLAHILSKRSAIALALAFGCMVYVDVGFKTWTPTFLIETFGFTPEKAGFSAVFYHF
                     VCAFVGVMIGGKLTDRWAAHRRTVRFEANAVGLLLGAPFILLMARSGSEWACFAALGL
                     FGLFRGVYDSNLFASLFDVIRPQYRASATGLMLSFAFLTGSLSPTVLGFFKARFGLSF
                     GLASLAFAYVLGGVIILTARLLFFGRDYEGRPAA"
     gene            1585..2940
                     /locus_tag="PLZ74_10760"
     CDS             1585..2940
                     /locus_tag="PLZ74_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOR98839.1"
                     /translation="MRMRKQGRVLIAVALCVAGAEAELPAAYRALWEAPELTRRIEEG
                     IERNRKGDLALRVTDAAGRPVAGAAVTVRQTAHAFLFGCNAFVLGQLETPERNRLYEE
                     RFARICNFATVPIYWKGTEPRQGVLRYAEGGENIWRRPPVDRFIPFGRKYGLTLKAHP
                     MLWHEHNPDWLPRDPEALKALYGKRFRELAGRYAKEITFWEVTNESAGCPKTYPLYSE
                     DRAYVAWALREAAPLFGPENRLMINDFTRFNALPPARNTYHQQLRGLIDQGLRLDAIG
                     LQFHIWFEPRLMERHLAGERYRPADLLDTYEAFAAFGRPVYITEITVPTPEGPDGEEV
                     QAAVIRNLYRLWFSAPAMAGVTYWNLGDSMAHEKENTARAGLVDSALNPKPSYRALDE
                     LVNRIWRSRAEGTTDAEGRLRFRGFYGTYEATVTQGGKTRRIPARWSKGGGPEQRVVM
                     D"
BASE COUNT          504 a          963 c         1077 g          562 t
ORIGIN      
        1 ccccgacgct gccaccctcc ccacgcgctt ctaccgcttc aacttcccgc agtagaagga
       61 ggggggcgac aggtgctcca gtctgcggca tcgcttgatc cgacgaatcc gcctgatctg
      121 actgatccgc cattgcgggc ggggacgccc gcgctcccga cacctgacgc cggttttttg
      181 cgcaaacgca tctggcttgg cggcgcgtga tgcggtacca tgctctcgga tcactaccaa
      241 ggggggaaga gatgtcggaa acccggaatc atgcggaaca cactgcgccc gcgtcttaca
      301 aatggctgct ggtggccctg ctctgggtcg tctttttcct gaatcagggg aaccggcaga
      361 tttacagcac cgtgttgccg cagatccggg ccgcgaccgc gcagggcggg ttggggctga
      421 gcgatgtgca ggccggattg gtcgccacgg tgttcatcgc ggtctacggc gtctgcgtgc
      481 cgctcgccgg atttctgggc gacatcctgc gccgcaagaa acagatcctg ttcagtctga
      541 tcctgttcag cgcggggacg ctgctcaccg ggttcagcgg cgggatgccg ggaatgatcc
      601 tcttttacgg catcctcttt ggcgggggtg aggcgctcta ctatccgccc gccacgtcgc
      661 tcttggggca gttccatgag aacagccggg ccacggcctt ttccatccat cagacagccc
      721 tgtacctcgg catcatgttc agcggctatt tttcggggta tctggcccgg acaatggcag
      781 gaggctggcg cgcgcctttt gtggtgttcg ggctggcggg gatcgtctgg agcggggtgg
      841 tgctgttcct catgcgggac acgccgtcgc caacgcgcga tgccgccgcg gcgcgtgtgc
      901 cgctgcgcga gaccctggcg catattctga gcaagcggtc cgccatcgcg ctggccttgg
      961 ccttcggctg catggtctat gtggacgtgg ggttcaagac ctggacgccc acgttcctga
     1021 tcgagacctt cgggttcacg ccggagaagg ccgggttctc ggcggttttt taccattttg
     1081 tctgcgcctt cgtgggcgtg atgatcggcg ggaaactgac ggaccgctgg gccgcgcacc
     1141 gcaggacggt ccggttcgag gcgaacgccg tggggctgct cttgggcgcg ccgttcatcc
     1201 tcctgatggc ccggagcgga tccgaatggg cgtgtttcgc cgcgctcggc cttttcggcc
     1261 tgttccgcgg ggtctacgac tccaacctct tcgcctcgct gtttgatgtg atccggcccc
     1321 agtaccgcgc ctccgcgacc gggctgatgc tctccttcgc gtttttgacg ggttcgctct
     1381 cgccgaccgt gctgggattt ttcaaggcgc gcttcgggct gtcgttcggg ctcgcctcgc
     1441 tcgcgttcgc gtacgtgctg ggcggggtga tcatcctgac cgcccgcctg ctgtttttcg
     1501 gacgcgatta tgagggcagg cccgctgcct gagcgcggat cgcacaaaaa ggatatggca
     1561 tcaagaacaa gaatgaggac gaggatgaga atgagaaaac aaggacgggt gctgattgcg
     1621 gtcgcgctgt gcgtggccgg tgcggaagcg gaactgcccg cagcctatcg cgcgctctgg
     1681 gaggcgccgg agttgacgcg gcggatcgag gaggggatcg agcgcaaccg caagggggat
     1741 ctcgccctgc gcgtgaccga cgctgcgggg cggccggtcg cgggtgccgc ggtgacggtg
     1801 cggcagaccg cgcacgcgtt cctgttcggg tgcaacgcgt ttgtgctggg tcagctcgag
     1861 acgcccgagc gcaaccggct ctatgaggag cggttcgccc ggatctgcaa cttcgccacg
     1921 gtccccattt actggaaggg gaccgagccg cggcagggcg tgctgcgcta cgccgagggg
     1981 ggcgagaaca tctggcgtcg gccgccggtc gaccgcttca tcccgttcgg caggaaatac
     2041 ggtctgacgc tcaaggcgca cccgatgctg tggcacgagc acaaccccga ttggctgccc
     2101 cgcgacccgg aggcgctgaa ggccctctac gggaagcggt tccgcgaact cgccggacgc
     2161 tacgcgaagg agatcacgtt ctgggaggtg accaacgaat cggccggctg ccccaagacc
     2221 tacccgctct actcagagga tcgggcgtat gtggcctggg ccctgcgcga ggccgcgccg
     2281 ctcttcgggc cggagaaccg gctgatgatc aacgatttca cccgcttcaa cgccctgccg
     2341 cccgcgcgca acacctatca tcaacagctc cgcgggttga tcgatcaggg gctgcgcctc
     2401 gacgcgatcg ggctgcagtt ccacatctgg ttcgagcccc gcctgatgga gcgccatctg
     2461 gctggcgaac gctaccggcc cgccgacctg ctcgacacgt acgaggcgtt tgcggcgttc
     2521 gggcgtccgg tgtacatcac cgagatcacg gtgccgacgc cggagggtcc ggacggcgag
     2581 gaggtgcagg ccgcggtgat acgcaacctc taccgcctct ggttcagcgc gccggccatg
     2641 gccggggtca cctattggaa tctgggcgac agcatggcgc acgaaaagga gaatacggcg
     2701 cgggccggtc tggtggacag cgcgttgaat cccaaaccct cctaccgcgc gctggacgag
     2761 ctggtcaacc gcatctggcg cagccgtgcc gagggtacga cggatgcgga ggggcgcctg
     2821 cggttccgag ggttttacgg gacgtacgaa gcgacggtca cgcagggcgg caaaacgcgc
     2881 cggatccccg cccgctggtc gaaaggcggg gggccggaac agcgcgtggt gatggattga
     2941 gggagggagg acaggagacg ggagacggga gacgggagtc gggagcgcgg gcgtccccgc
     3001 ccgctggccg aaaggcgggg ggccgctgaa cgtcgacacg aaaaacgctg cacaggacaa
     3061 aacaatgaat ccgaacaagc agacaatgtt gaagcaggct ttttca
//
LOCUS       DAOSRB010000280         3062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_28902_length_3062_cov_6.572664, whole genome shotgun sequence.
ACCESSION   DAOSRB010000280 DAOSRB010000000
VERSION     DAOSRB010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28902_length_3062_cov_6.572664"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLZ74_10765"
     CDS             complement(<1..188)
                     /locus_tag="PLZ74_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98840.1"
                     /translation="MLDVSFPPGTVLPIVSATSVTAMDPNAWQVAGSIISRKSVRLVF
                     GPTGISLAINARGTRVLFR"
     assembly_gap    220..229
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(488..1240)
                     /locus_tag="PLZ74_10770"
     CDS             complement(488..1240)
                     /locus_tag="PLZ74_10770"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOR98841.1"
                     /translation="MRNQNRMWLGLVVLVSGCSLTSPEKPDADTCCPKTASCQTCPVR
                     TGEPLHPDSRNWRPLFHLNLANAVFTPGVWFINDKGDLTATKDEAIWTDRDFENFILD
                     LEYSCDPAANSGVLLYCSDMKNWIPNTVEVQILDDNHPHWVNEKPFAKNGGLYGHLPP
                     KVNNVKPAGEWNRMTVTARGKRIKVAVNGEITVDTDLSQWTSAKKNPDGTDIPPWLNR
                     PWAELPTRGRIGLQGKHGNASIRFRFIRIREL"
     gene            complement(1329..2849)
                     /locus_tag="PLZ74_10775"
     CDS             complement(1329..2849)
                     /locus_tag="PLZ74_10775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR98842.1"
                     /translation="MKIAIIHQAVPDTAAADERDVLVQAAAVRTALTTRGHTVTVLAC
                     TTDLGNVRRKLELLMPDCVFNLVETLDGSGRLIHLAPAVWEAAGIPFTGSSAEALYLT
                     TQKRLAKERMLAAGLPVPEWIEAGDGRAPHAVPRADWIVKSIWEHASVGLDDNSILRG
                     LTPEEVAAHLPEGSFAERFIDGREFNLALLAGPDGPVALPPAEIVFENFPAGKPRIVG
                     YQAKWDEASFEYAHTVRRFADPSEDGVLLAELKCLASRCWELFRLDGYARVDFRVDGT
                     GRPWILEVNANPCLAPDSGFAAALDRAGIAYADAMERIVRAAVKAERPSGNSCGAPAG
                     NVYRRPCPDSPSVTFRYEVLPADVDTIREITVATGFFHDYEIPVAVELAEERLAKGTA
                     SGYEFVLAECDGKVIGYTSFGPIPCTRSSFDWYWLAVRPEFQGFGFGRRLLEQVEARV
                     RAMGGTRLYCETSGRPQYAATRCFYERMGFELGELLDNYYDVGDGRATYVKRLDQV"
BASE COUNT          624 a          999 c          877 g          552 t
ORIGIN      
        1 cggaacagga cccgcgtccc gcgcgcattg atcgcaagcg aaattcccgt cggaccgaac
       61 acgagacgga cggatttacg cgaaatgatc gatccggcaa cctgccaggc attcggatcc
      121 atcgccgtga ccgacgtcgc cgacacaatc ggcaggaccg tccccggcgg gaacgacaca
      181 tccagcacct accctatcgc tgggagcatg cacaatggcn nnnnnnnnnc acgaacccct
      241 agtcatttct cacgaactcc aaacagtacc cctgatccag ccgcccccct ccttacttcg
      301 ggtggacggt ccaagacgga ctccggttag gaagaaccat ccgcgcgtgt ggtggcggac
      361 accccggaag atgcgtctcg ggtttctgct tcgtgtatgt gcaccgctgc gctgtgaacg
      421 cgtacggggc atcaaacacg caacatgcag attatcggca gagacgcctc cccgaaccgt
      481 ccgcagatta caactcgcgg atgcggatga agcggaagcg gatcgaggcg ttgccgtgtt
      541 tgccctgcaa accgatacgg ccccgggtcg gcaactctgc ccaaggacgg ttcagccagg
      601 gcgggatgtc cgtcccgtcg gggtttttct tggccgaggt ccactgcgaa agatcggtgt
      661 ccacggtgat ctcgccgttg accgcgactt tgatgcgctt ccctcgcgcg gtcacggtca
      721 tgcggttcca ctcgcccgca ggcttcacgt tgttgacctt cggcggcaga tgaccgtaga
      781 gtccgccgtt cttggcgaaa ggcttctcat tgacccaatg cggatggtta tcgtcgagga
      841 tctgcacctc caccgtgttc gggatccaat tcttcatgtc ggaacagtag agcagcacgc
      901 cgctgttcgc ggccggatcg caggagtatt ccagatcaag gatgaaattc tcaaagtcgc
      961 gatccgtcca gatcgcctca tccttcgtgg cggtcagatc tcccttgtcg ttaatgaacc
     1021 agacaccggg ggtgaacacg gcattcgcca gattaaggtg gaaaagcggc cgccagttcc
     1081 gggaatccgg atgcagcggc tcccccgtgc gcactggaca ggtctggcac gacgccgtct
     1141 tcgggcaaca tgtgtccgca tcgggttttt ccggagaggt cagcgaacac cctgacacca
     1201 gcacgaccaa ccccagccac atcctgtttt ggtttctcat cccgactctc cttacgcggt
     1261 tccgaattgc cgtaaagctt acgaagcttg ggtcataatt tcaatcccaa tccggtcgcg
     1321 gaaaccgttc atacctgatc cagccgcttc acataagtcg cgcggccgtc gccaacgtca
     1381 tagtagttgt cgagcagttc gcccaactcg aatcccatcc gctcataaaa acagcgggtc
     1441 gcggcatact gcggacggcc cgaggtttcg caatagagac gtgtgcctcc catcgcgcgg
     1501 acccgcgcct cgacctgttc gagcaaccgc cgcccaaatc cgaaaccctg aaactcggga
     1561 cgcaccgcca accagtacca gtcgaaactg ctccgggtac aggggatcgg accgaaagaa
     1621 gtgtatccga tcaccttccc gtcgcactcg gccagcacga actcgtatcc cgaggccgtg
     1681 cctttggcaa ggcgttcctc ggccaactcc accgccacgg ggatctcata atcgtgaaag
     1741 aacccggtcg cgacggtgat ctcgcgaatc gtgtccacat ccgcaggcag cacctcgtag
     1801 cggaaggtga cggacggaga gtccgggcat ggcctgcggt acacgttccc ggcgggtgcg
     1861 ccgcacgaat ttccggacgg acgctccgcc ttcaccgcag cccgcacgat ccgttccatg
     1921 gcgtcggcat aggcgatgcc cgccctgtcc aacgcagcgg cgaaaccgct gtccggcgcg
     1981 aggcaggggt tggcgttgac ctcgaggatc caaggccgcc ccgtgccgtc cacgcggaaa
     2041 tccacgcgcg cgtacccgtc gaggcggaaa agctcccagc aacgcgacgc gaggcatttc
     2101 aattcagcga gcaacacgcc gtcctcagac ggatcggcga aacgccgcac cgtgtgcgcg
     2161 tattcgaacg acgcctcatc ccacttggcc tgatagccca caatgcgcgg tttcccggca
     2221 gggaagtttt cgaacacgat ctctgcgggc ggcagcgcca caggaccgtc cggcccggcg
     2281 agcagagcaa gattgaactc gcggccatcg ataaaacgtt cggcaaagct tccttccgga
     2341 aggtgtgccg caacctcctc cggcgtcaaa ccgcggagaa tgctgttgtc gtcaagcccg
     2401 accgaagcat gttcccagat ggacttcaca atccaatcgg cccgcggcac ggcatgcggg
     2461 gcgcggccgt ctccggcttc gatccactcc gggaccggca accctgcggc gagcatgcgt
     2521 tccttggcga gtcgtttctg agtcgtaagg tagagggctt ccgcagaact gccggtaaac
     2581 ggaatgcccg ccgcctccca cacggccggg gcaaggtgga tcaggcgtcc ggaaccgtcg
     2641 agcgtctcaa ccagattgaa gacacagtcc ggcatcagca gttccaactt acgccgcacg
     2701 ttgccgaggt ccgtggtaca ggcaagcacc gtgacggtgt ggccgcgggt ggtcagggca
     2761 gtgcgcacgg ccgctgcctg aaccagcacg tcgcgctcgt cggccgccgc agtgtcggga
     2821 acagcttgat gaatgatcgc gattttcatg cgttttccag taccctttcc aaaacggatc
     2881 gcggccgtca cacgttgtgc cccaccgcat cgacggtccc gtccaatcgg ttgggaatcc
     2941 agtgtagagc cggagccggg attgcgcaag agcgcaacac gattttcccg aggccgggcg
     3001 tacgaatccg gccacaaatc gcattacact ttgaacacca agatacccct gcttttcatg
     3061 aa
//
LOCUS       DAOSRB010000281         3014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_29522_length_3014_cov_6.982089, whole genome shotgun sequence.
ACCESSION   DAOSRB010000281 DAOSRB010000000
VERSION     DAOSRB010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29522_length_3014_cov_6.982089"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1270)
                     /locus_tag="PLZ74_10780"
     CDS             complement(47..1270)
                     /locus_tag="PLZ74_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98843.1"
                     /translation="MTLTRRGFFGGSIAVAATSGLKGAVPSPAGKLQGFDESPDAKTG
                     DPWEPFSDKKVRVGIAGYGVCAFGATFSFQDHPNVEVVAAADLFKDRAEMLAKVTGAK
                     RIYLSCEEMVDKEGKNMDAVFITTDAPSHARLSIQALNRGYHVAVAVPAVFGEDQLAL
                     AEELFATVKKTGLVYALFETTAFRPQCFAMRNIYKAGGFGDMVYTEGEYFHFSPPGKP
                     IDSYKGWRIGLPPQYYPTHSNGFYTCVTHKAFTEVTCIGKPSIQPAYQPGGNRYKNPY
                     GSEIALFKTEEGGSARMAVAWDLKGYSGEVGRCYGQLGCFRERYVGDNRLIKDAKLIR
                     TALPPGVENNGHHGGSHPYLTDDFIRAILLKRNPCVDVATALNTTVSGIYAHMSAMKG
                     GETLKIPVYKLLPIN"
     gene            complement(1337..2881)
                     /locus_tag="PLZ74_10785"
     CDS             complement(1337..2881)
                     /locus_tag="PLZ74_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOR98844.1"
                     /translation="MTTRRVVCVSVLLSAGVVFGWWDHPYITRCAWDAQPKELTAPYT
                     DFERDEFCYHTMMSDYPRMATAGDFGYFQLWDFFMWWQRRARVDYANGGGEDMSFQRV
                     LQAVKTETPLNALLWAGCFFHALEDSMSISHTALPNEYQGEWHGPCEAFAHWEKFNIG
                     GYVAKPLAGGYDPAALRKELDRRIAEVNKRAYDLWPKMKLIIDENGIKQPPAVKAQTN
                     ERIDPYVMTFAEGVAKLAADTLCTFLNARREFAENTGAALEGAINAPPDPVHAQRTAK
                     VYVRTVDDAPTHFETMAFNGRYAFRNLPDGTYRLAATRLGAKNALSAPFELKKERVTR
                     IDLALETELPQGNLVWNPTGTCDLYGARSPDRWRMKPLFNWGPLQQDYWRTSFMHLEP
                     GWTYRCGAKLKKPTTVRFRFAKSYGGHGPALELDLKDRLESEMVWTNAENFFKAKIEV
                     VSTEPLTNLIEKVWFVPVPPKDWAGIYPMSDYVPVDDIFRQSLRMTPPQPEFWRRDPC
                     ARPPAK"
BASE COUNT          570 a          929 c          841 g          674 t
ORIGIN      
        1 ttcaactgct ccttttgggt tcaatcctga aaatcctgtc atcctgtcaa tttataggca
       61 agagtttgta tacggggatc ttgagggtct cgccaccctt cattgcgctc atgtgcgcgt
      121 agatgccgga gaccgtggtg ttgagcgccg tggcgacatc cacgcagggg ttgcgcttga
      181 gcaggatcgc ccggatgaag tcgtccgtaa ggtaggggtg cgaaccgccg tggtggccgt
      241 tgttctccac gccgggcggc agtgccgtac ggatcagctt cgcgtctttg atcagccggt
      301 tgtctcccac gtagcgttcg cggaaacagc ctagctgtcc gtagcagcgt cccacttcgc
      361 cggagtatcc cttgagatcc caggcgacgg ccatacgcgc cgagccgcct tcctctgttt
      421 taaaaagcgc gatctcgctt ccgtagggat tcttgtaacg attgcccccg ggctggtatg
      481 ccggctggat gctcggcttt ccgatgcagg tcacttccgt gaatgccttg tgcgtcacgc
      541 aggtgtaaaa accgtttgaa tgcgtggggt agtattgcgg cggcaagccg atacgccagc
      601 ccttgtaaga gtcgatgggt tttcccggcg gagagaaatg gaaatactcg ccttccgtat
      661 acaccatgtc cccaaatccg cctgctttgt aaatgttacg catcgcaaag cattgcggac
      721 ggaacgcggt cgtctcgaaa agcgcgtaga cgagtcccgt tttctttacc gtcgcgaaaa
      781 gctcttctgc gagagcgagc tgatcctcgc cgaaaaccgc cggcacagcg accgccacgt
      841 ggtacccgcg gttcagcgcc tggattgaga ggcgcgcgtg gctcggcgcg tctgtcgtga
      901 tgaacacggc atccatgttc ttcccttcct tatcgaccat ctcctcacag gaaaggtaga
      961 tgcgtttcgc gcccgtcacc ttggcgagca tctcagcccg atctttgaag agatcggctg
     1021 cggctaccac ttccacgttc ggatggtctt ggaaggagaa agtcgccccg aatgcgcaca
     1081 cgccatagcc ggcgatgccc acgcgcactt tcttgtctga gaacggttcc cacgggtctc
     1141 ccgttttcgc atccggcgac tcgtcgaatc cttgcagctt ccctgcgggt gacggtaccg
     1201 cgcccttgag cccggacgtt gccgcaacag cgattgatcc cccgaaaaaa cctcttcgcg
     1261 tgagtgtcat cagtccctcc ctttcaatga atttaccaca gaggtggtac gatagccatt
     1321 gcgtattgct cgaaagtcat ttcgccggtg gccttgcaca cgggtcgcgc cgccaaaact
     1381 cgggctgtgg cggtgtcatc ctgagagatt ggcggaaaat gtcatccaca ggcacgtagt
     1441 cgctcatcgg gtagattcct gcccagtcct tcggcggcac gggcacgaac cagaccttct
     1501 cgataagatt cgtcaacggc tctgttgaaa cgacctcaat tttcgcctta aaaaagttct
     1561 ccgcattcgt ccagaccatt tcggactcaa gcctgtcctt gaggtcgagt tcaagcgccg
     1621 gaccatgtcc gccgtaactt ttggcaaaac ggaaacggac ggtggtcggc ttcttgagtt
     1681 tcgcgccgca acggtacgtc cagcctggct ccaggtgcat gaacgatgtg cgccaataat
     1741 cctgctgcag cggcccccag ttgaagagcg gcttcatgcg ccagcgatcg ggagacctgg
     1801 cgccgtagag atcgcaagtg cccgtcggat tccagacgag gttgccctga ggaagctccg
     1861 tctcgagtgc cagatcgatt cgcgtcacac gttccttctt cagctcgaac ggcgcggaga
     1921 gcgcgttctt cgcgcccaga cgcgtcgccg cgaggcggta cgtgccgtcc ggcagattcc
     1981 gaaaggcata gcgcccgttg aatgccatgg tctcgaaatg cgttggcgcg tcatccaccg
     2041 tgcgcacata caccttcgcc gtgcgctgcg catggacggg atcgggcggc gcgttgattg
     2101 cgccctccag cgccgcgcca gtgttttccg cgaactcgcg tcgcgcgttc agaaacgtgc
     2161 acaacgtgtc cgcggcgagt ttcgcgacac cctccgcgaa agtcatcaca tacggatcga
     2221 tgcgttcgtt cgtctgggct ttgacggccg ggggctgttt gatgccgttt tcgtcgatga
     2281 taagcttcat cttcggccag aggtcatagg ccctcttgtt cacttccgca atccgccggt
     2341 ccagttcctt cctgagagcc gccgggtcgt atccgcccgc gagcggcttc gcgacatagc
     2401 cgccgatgtt gaacttctcc caatgggcaa acgcttcgca cgggccatgc cattccccct
     2461 gatattcgtt gggcagggct gtgtgcgaaa tggacatcga atcctccagc gcatggaaaa
     2521 agcagccggc ccatagaagc gcgtttagag gcgtctcggt cttcaccgcc tggagaacgc
     2581 gttggaacga catgtcttct ccgccgccat tcgcgtagtc gacgcgggcg cgacgctgcc
     2641 accacatgaa aaagtcccac aactggaagt agccgaaatc acccgctgtc gccatgcgcg
     2701 gatagtcgct catcatcgtg tgatagcaga actcgtcccg ctcaaagtcg gtgtagggcg
     2761 ccgtcagctc cttcggttgc gcatcccacg cgcatcgcgt gatataggga tgatcccacc
     2821 agccgaacac cacgcctgca gaaagcaata ccgaaacgca gacaactctt cgtgttgtca
     2881 tgtaagtcat ccttgttttc ctatgaacaa ttcttccacc tcacatgcct ccgctctccg
     2941 tttcccactc gccgtacgct tccggttcct cgcggacgtt gctttcacgg tgggctttca
     3001 acaccagcat gtcc
//
LOCUS       DAOSRB010000282         3009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_29588_length_3009_cov_7.051794, whole genome shotgun sequence.
ACCESSION   DAOSRB010000282 DAOSRB010000000
VERSION     DAOSRB010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29588_length_3009_cov_7.051794"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..2460
                     /locus_tag="PLZ74_10790"
     CDS             247..2460
                     /locus_tag="PLZ74_10790"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOR98845.1"
                     /translation="MRRWICFFGWCGAAALQSAALTPADLMCELLAKPGLVAVTDATP
                     DFSWGFQDVRPGDFQSAYQIQVASGTAFLKTDQPDLWDSGKVASGQSLYIAYAGKALP
                     TDAEVCWRVRVWDGRGKEGPWSHTYSFKTAPVLSGDSALRYPLAQEWVKPVRVVTNAQ
                     GRVLVDFGRAAFGWVELVPPHELRRGGPFTLHLGEKLKGDSVDAKPGGTIRYAKVNGA
                     LTSPNVYRVPLVADKRNTSGGKEGAAILLPPEFGVVMPFRYVEIEECPYTVTPDTIRQ
                     IAVFYPFDMEAARFVSSDAVLDRVFDFCKYSIKATTFAGVYVDGDRERIPYEADAYIN
                     QLCHYCTDREFTLARYSHEYLMAHPTWPTEWKQHSVMMAWADWMYTGNTESLERMYDR
                     LKAEKTLEKNARASDGLLVTGGPSAPMESGLRDITDWPVGERDGFVFKPVNAVVNAFY
                     CLNLRQMAEMAAALDKTADAEAYRAKHERAVKAFNALFFHAERGCYVDGEGATHASLH
                     ANMLPLAFGLVPEAERGRVTRFVVSRGMACSVYGAQYLLEALFEADQDDTAIHLMTRD
                     ELRSWVNMMKAGSTITMEAWDIMVKPNLDWNHAWGAVPANIIPRFVMGVRPIEPGFGK
                     ILIRPQVGSLEAIEGYVPTVRGTVRVGVRQKPGTQYAVTVEIPVNTTARVELPWCEGA
                     RLRVNGKKVAARVEGGRFVVDDVTSGRHTLVWQCGEEAACGSSFGRRVRRIFGGG"
     gene            2409..2813
                     /locus_tag="PLZ74_10795"
     CDS             2409..2813
                     /locus_tag="PLZ74_10795"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOR98846.1"
                     /translation="MRFEFRQASPEDLRRWLTFLGAALLIALGVVALDAAGVRLCLFR
                     RLTGLPCLTCGGTRAFAALATGDVTGAFRQQPLVSALLTVGTAAGAVHSLLLARGRHL
                     TLRTSASERRLLLLAAVILAAANWVYLLWRGA"
BASE COUNT          536 a          859 c         1040 g          574 t
ORIGIN      
        1 gtctcgggac gccctcggtg tccagtattt gacggatcgt caccgcctca aaaaaaccta
       61 cggcgtcttt tctatttgac ggaccacgga tcaggacgca ttcctgctct cccgcagatt
      121 ccgattgccc gcaaagcccc aaacctgtta cgttgggttc atcccttcat ttagaaaacc
      181 cccatgcgaa tgtggcgggt ttcaaggccg gcatgctgct ggcaccgtga gcggaaagga
      241 aacgcgatgc gacgttggat ctgttttttc ggatggtgcg gggcggcggc gctgcaaagc
      301 gccgcgctca ccccggctga cctgatgtgc gaactgttgg ccaagccggg gctggtcgcc
      361 gtgaccgacg cgacgccgga tttttcctgg gggtttcagg acgtgcgccc cggcgacttc
      421 caaagcgcct accagattca ggtggcgtca ggaaccgcgt ttctgaaaac cgatcaaccg
      481 gatctctggg actcgggcaa ggtcgcctcg gggcagtcgc tttacatcgc ctacgcgggc
      541 aaggcgctgc ccaccgacgc tgaggtgtgc tggcgggtgc gcgtgtggga cggccgcggc
      601 aaggaagggc cttggtcgca tacgtactcc ttcaaaaccg cgcccgtatt gagtggagat
      661 tcggcgttgc ggtatccgct cgcgcaagag tgggtgaagc cggtccgcgt ggtcaccaac
      721 gcgcaggggc gcgtgctggt cgatttcggg cgggcggcct tcggttgggt cgagctggtt
      781 ccgccgcacg aactgcggcg gggcggcccc ttcacgctgc atctcggcga aaagctgaag
      841 ggcgacagcg ttgacgccaa accgggcggg acgatccgtt acgcgaaggt gaatggggcg
      901 ttgaccagtc cgaatgtcta ccgcgtgccg ttggtcgccg acaaacgcaa cacgagcggc
      961 ggcaaagagg gtgcggcgat tttgctgccg cctgagttcg gcgtggtgat gccgttccgc
     1021 tatgtcgaga tcgaggagtg cccgtacacg gtgacgcccg acacgatccg ccagattgcg
     1081 gtgttctatc cgttcgacat ggaggcggcg cgttttgttt cgagcgatgc cgtgctcgac
     1141 cgcgtgttcg atttctgcaa gtacagcatc aaggccacga cctttgcggg cgtgtatgtg
     1201 gacggtgacc gcgagcgcat cccgtatgag gccgatgcct atatcaacca actctgccac
     1261 tactgcaccg atcgcgaatt cacactggcg cgctacagcc acgagtatct gatggcgcat
     1321 ccgacatggc cgacggaatg gaagcagcac tcggtgatga tggcgtgggc ggattggatg
     1381 tacaccggca acacagagtc gttggagcgc atgtatgacc gcctcaaagc cgaaaagacg
     1441 ctggagaaaa acgcgcgcgc ctcggacggg ttactggtca ccggcggccc ctccgcgccg
     1501 atggaaagcg ggctgcgtga catcacggac tggccggtcg gcgagcgtga cgggtttgtg
     1561 ttcaaacccg tcaatgccgt ggtcaacgcc ttctactgcc tgaacctgcg tcagatggcg
     1621 gagatggccg cggcgctgga taagacggcg gatgccgagg cgtaccgtgc gaaacacgag
     1681 cgggccgtca aggcgttcaa cgcgctcttc ttccatgccg agcgcggctg ctacgtggat
     1741 ggcgaaggcg ccacccacgc ctcgctgcac gccaacatgc tgcccttggc gttcggcttg
     1801 gtcccggagg cggaacgcgg acgggtgaca cgctttgttg tctcgcgcgg gatggcgtgc
     1861 agcgtgtatg gcgcgcagta tctgctcgag gcgctcttcg aggccgatca ggatgatacc
     1921 gcgatccacc tgatgacgcg cgacgagctg cgtagctggg tcaacatgat gaaggccggc
     1981 tcgaccatca cgatggaggc ctgggacatc atggttaagc cgaatctgga ctggaaccac
     2041 gcatggggag cggtgcctgc caacatcatc ccccgctttg tcatgggcgt ccggccgatc
     2101 gagcccggat tcggaaaaat cctgatccgg ccgcaggtcg gttcgctgga ggcgatcgaa
     2161 ggctatgtgc cgaccgtacg gggcaccgtg cgggtgggcg tgcgccaaaa accggggaca
     2221 cagtacgccg taacggtaga gatccctgtc aacacgaccg cgcgcgtcga gttgccgtgg
     2281 tgtgaaggcg cgaggctccg ggtgaacggc aagaaggtgg cggcacgggt ggaaggcgga
     2341 cgctttgtgg tggacgacgt gacgtcggga cgacatacgc tggtatggca gtgcggggag
     2401 gaggctgcat gcggttcgag tttcggcagg cgagtccgga ggatcttcgg cggtggctga
     2461 cgtttctggg cgccgctctt ctgatcgcgc tgggtgtggt cgcgctggac gccgccgggg
     2521 tgcggctttg tctctttcgc cgtttaaccg gcctgccctg tctgacctgc ggcggcacgc
     2581 gcgcgttcgc ggcgctagcg accggtgatg tgaccggcgc gttccgtcag cagccgctcg
     2641 tgagcgcact gctgacggtg gggacggccg ccggtgccgt ccacagcctg ttgctggccc
     2701 ggggcagaca tctgacattg cggacatccg cgagcgaacg aaggctattg ctgttggccg
     2761 ccgttattct ggcggcggcc aactgggtat acctgctctg gcgcggcgca tgaaaccgtt
     2821 gccgggaagg ggcaggcgga gtttggattt aggatttagg atttaggatt taggagttag
     2881 cccgccgccc gcgcttcgca cgggaatgta actgaggata acgttgatgt gggttatagg
     2941 caaagaggga ggatttagcc cgccgcgcgg ctcagccgcg aaaaaacctt ggaatacgtt
     3001 taggacatg
//
LOCUS       DAOSRB010000283         2984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_29912_length_2984_cov_9.392284, whole genome shotgun sequence.
ACCESSION   DAOSRB010000283 DAOSRB010000000
VERSION     DAOSRB010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29912_length_2984_cov_9.392284"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            570..1316
                     /locus_tag="PLZ74_10800"
     CDS             570..1316
                     /locus_tag="PLZ74_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOR98847.1"
                     /translation="MVGSFLLPAGLPLASLIDGFLISNLMGKSIVLVQLASSIVAAAI
                     IIGKNKELNGIVRNSRRFQREFLLGHDVLDYYLRRKKGAMTAIELIYDRTCERMVKLL
                     DPDMRRSVIGRQSDANQAALSAREIELVRSTCEHTVQEEEIRIEHGMGLLATIVTASP
                     MLGLLGTVWGVLDAFADMGTKGTVLLSEIAPAISSALVTTVVGLLVAIPSAIFYNSLA
                     AKVRQLSSDMVGFADELMGRVACEFQGRSD"
     gene            1316..1726
                     /locus_tag="PLZ74_10805"
     CDS             1316..1726
                     /locus_tag="PLZ74_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOR98848.1"
                     /translation="MGLRRLARKKDSKIINNIDMTSLIDLTFLLLITFIITMPAIEQG
                     ISVKLPQGKTDTLPVKKSNVVTLDVEGRTYLNNREITLEDLERTLGNLAAEDPEVAVL
                     VRGDERLEYGKVVQVMKILYKVRITRMALVTQSD"
     gene            1734..2621
                     /locus_tag="PLZ74_10810"
     CDS             1734..2621
                     /locus_tag="PLZ74_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOR98849.1"
                     /translation="MKTGPVNSYSLKWSMVLHGVLLTVAVVMPLVPGCRPKELTIPVD
                     FTVVLEENLVEPNVPDDVPEPEPTPDPPKPEPLPDPPKPEPLPDPPKPQPLPDPPKDV
                     VATEKKPDPPKKKPDPPKKQPEKKPTPPKPKPPPKKPEFQKGKRVEAAKPKQPRFEDL
                     YKPYDPSKPTSTKPVTDKKLSRAEIEKALRAGARPGTRNIIPEDEISRCVLLVRRALY
                     DAWVQPGNADAGPRPALLDIRLDGAGRIVSYRIRQSSGSAYFDQTVLKAAANAQPIRG
                     LSSDFLKQYETLTVEFKLE"
     gene            2716..>2984
                     /locus_tag="PLZ74_10815"
     CDS             2716..>2984
                     /locus_tag="PLZ74_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98850.1"
                     /translation="MRRLGCVLVAVIGFQALFTGCKCGFEAKRGMERKPDVLAVLPAY
                     CPTLDGMAIDAKGNLIVACPNFVDQSQPACLIKIDKKTLKVERWVT"
BASE COUNT          650 a          810 c          905 g          619 t
ORIGIN      
        1 agaccacatg catctgatcg gtgtcggcat acggcacacg gtaggggatg gtgacgcgtt
       61 tcggttcaga catctgtagg ctcctttgtt tgattcgggg caagtgtaag gggcacggtc
      121 ggcaaaagca actcccgata taaacccccc aagcatcgca agtgaaaacg cagtgtaaaa
      181 agtaatcggt tggtgacggg gggatgaagc tcgtgcgttt ctgatttcat tacgaaccgc
      241 tgttgtgtgt gtggactagt gtttttcgac gttaaaggcc aaactccaga gggtcacata
      301 ttcgaatatg tgacccgggg gtgcgcaagg ggttcgctgt gtgcgtcgcc tgtgccctgt
      361 cgacacggca tctggttcgc ggggtacgga aaaacgctca actctcaacg ttcaagaaaa
      421 tgcgcacgca caaaacatgc gcttgactcg cgtgcctcct gccactgatc gattactttt
      481 tcgacaacgc aggattgaca tccggcgcgg cgtggcgcat gctacagata tccctttatc
      541 ttatcccttt ggctaaggag gccacgcgaa tggttggttc atttctgctg ccggcgggtt
      601 tgccgctggc ttctttaatt gacggttttc tgatatcaaa cttgatgggg aaaagcatcg
      661 tgttggttca gcttgccagt tcgatcgtgg ctgccgcgat catcatcggc aagaacaagg
      721 aactgaacgg catcgtgcgt aacagcaggc gtttccagcg tgagtttctg ctcggccacg
      781 atgtactcga ttattatttg cggcgcaaaa aaggtgcgat gaccgcgatc gaattgatct
      841 atgaccgtac ctgtgagcgc atggtcaaac tgctcgaccc cgatatgcgg cgctcggtca
      901 tcggtcgcca gagcgatgcc aaccaagcgg cgctttcggc ccgtgagatc gaattggtgc
      961 gcagcacctg tgagcatacc gtgcaggaag aagagattcg catcgagcac ggcatgggcc
     1021 tgctggcgac gatcgtgacc gcctcgccga tgctcggtct gctcgggacg gtgtggggcg
     1081 tgctggatgc cttcgccgac atgggcacca agggtacggt gttgctgtcg gagatcgccc
     1141 ccgcgatctc atcggcattg gtcaccaccg tcgtgggatt gctggtggcg atccccagcg
     1201 cgatcttcta caactcgctc gccgccaagg tccggcaact ctcatccgac atggtcgggt
     1261 tcgccgatga actgatggga cgggttgcct gcgaattcca agggaggagt gactgatggg
     1321 gctgcgacgt ctggctcgca agaaagattc gaagatcatc aacaacatcg atatgacgtc
     1381 gttgatcgac ctgacctttc ttttgctgat tacctttatc atcacgatgc ccgcaatcga
     1441 acaggggatc tcggtaaagt tgccgcaggg caaaacggat acgctgccgg tcaaaaagtc
     1501 gaatgtcgtc acgcttgatg ttgaggggcg aacctatctc aacaaccgcg agatcacgct
     1561 ggaggattta gagcgtacgc tgggtaacct cgcggcggaa gatccggagg tcgcggtgct
     1621 cgtgcgcggc gatgagcgtc tggagtacgg caaggtggtc caagtgatga agatccttta
     1681 caaagtacgg atcacgcgca tggcgctggt cactcaatcg gactgagcgg cgcatgaaga
     1741 cgggaccggt taattcctac tcactcaagt ggtcgatggt gctgcacggc gtgctgctga
     1801 cggtggcggt cgtgatgccg ttggttccgg ggtgccgtcc caaggaactc acgatcccgg
     1861 tcgatttcac ggtggtgctt gaagagaacc tcgtggagcc gaatgtgccg gacgacgtgc
     1921 cggaaccaga gccgactccc gatccgccca agcccgagcc tctgcccgat ccgcctaagc
     1981 ccgagccatt gccagatccg ccgaaacccc aaccgctgcc cgacccgccc aaggatgtgg
     2041 tggccaccga aaagaagccc gacccgccca agaaaaagcc tgatccgccc aaaaagcaac
     2101 ccgagaagaa accgactccc cctaaaccca aaccgccgcc gaaaaagccg gagttccaga
     2161 aagggaagcg ggtggaggct gccaagccca agcagccgcg ctttgaagat ttgtacaagc
     2221 cgtatgatcc gagcaagccc accagcacca agccggtgac cgacaaaaaa ttgtcacggg
     2281 cagagatcga gaaggcattg cgcgccggtg cgcgtcctgg tacgcgcaac atcatccctg
     2341 aggatgaaat ttcgcgttgc gtgctgttgg tgcggcgtgc gctttatgac gcctgggtgc
     2401 aaccgggcaa cgccgacgcc ggtccgcgcc cggccttgct ggacatccgt ttggacggcg
     2461 cgggccggat tgtcagctac cgcattcggc aatcctccgg cagtgcttac tttgaccaga
     2521 ccgtgctcaa ggccgccgcc aatgcgcagc ccattcgcgg gctctccagt gactttctca
     2581 agcagtacga aaccctgacg gtcgagttca agttggagtg attcggcgcc ccaaaccttg
     2641 acatggcggc ttggacgtgc taattgtgcg gacaatcaaa ggtcgatggt cagttaaaag
     2701 aacggaggag gggagatgag acgtctcgga tgtgtgctgg tcgcggttat tggctttcaa
     2761 gccctgttta cgggatgcaa atgcggcttt gaagcgaaga gggggatgga gcgcaagccg
     2821 gatgtgctcg cggtgctgcc tgcatattgc ccgacccttg acgggatggc gattgatgcg
     2881 aaaggcaacc tcattgtggc ctgcccgaac tttgtcgatc agtcacagcc tgcttgcctg
     2941 ataaagatcg acaagaagac tctgaaggta gagcggtggg tgac
//
LOCUS       DAOSRB010000284         2983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_29929_length_2983_cov_5.018443, whole genome shotgun sequence.
ACCESSION   DAOSRB010000284 DAOSRB010000000
VERSION     DAOSRB010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29929_length_2983_cov_5.018443"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLZ74_10820"
     CDS             complement(<1..783)
                     /locus_tag="PLZ74_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOR98851.1"
                     /translation="MSDGIQPDQQTDQGLQNINIEDEMSSSYIDYSMSVIIGRALPDA
                     RDGLKPVHRRVLYAMNELGNTSNKPYKKSARVVGDVIGKYHPHGDQAVYDTIVRMAQE
                     FSMRYPLVDGQGNFGSIDGDPAAAMRYTEVRMDRLAEEMLDDLEKETVDFKPNYNEEF
                     QEPTVLPARLPNLLLNGSTGIAVGMATNIPPHNLGELVDGLTHYIENPECTIDELMQF
                     VKGPDFPTGAILCGTRGIEAMYKLGRGSLTIRGKAEIVEYKNK"
     gene            926..1891
                     /locus_tag="PLZ74_10825"
     CDS             926..1891
                     /locus_tag="PLZ74_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98852.1"
                     /translation="MDNRTPCPICRTPNISYASAGANTSEINCPRCGRYQIQAFAETQ
                     LHNSPPAFPPAHLLSGLCRNTWDHLGEKVLITVDLFKSWADLERVAKLPVPRDTDVVT
                     KGEYLLKHLRRRSKTLADRVSFTPHELACGFCSTKNELLFCLRYLADRGWVEECSAAN
                     RKGPPRDAITFRLTPAGWAALAAPATPAQPRAVVSVMLDREDESLWTQGFAAAAQAAG
                     YVAERVENREQLNKINDELIVSLRRAAFVIADLTGQAPMTYFVAGFAGGLGKPVFWTC
                     EESEARDKRLWLETRQSIITTWTRDKPEVFAARLAQRIEATIPGE"
     gene            complement(1910..2809)
                     /locus_tag="PLZ74_10830"
     CDS             complement(1910..2809)
                     /locus_tag="PLZ74_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOR98853.1"
                     /translation="MSQSKSIRWLGFLTLGVNISLMLVKITVGLMGNCYALIADGVES
                     LGDIITSSVTWLGFHFSLRPADACHPYGHGKIESLAGVFSGFSLLAVALLIAFQAVRE
                     LLTPHASPAWYTLPVLLMVVLVKEAVSRAVLRAGRATDSLALEGDAWHHRSDALTSAA
                     AAVGISLALLGGPRFAQADDWAALLACGIIVFNAVRILRKAFHEMLDGDVGDEMRFRV
                     MGEARGVPQVDSVSACRIRKSGTDYFVEVHIQVPGDLSVREGHAVGHQVRDAIMAAKS
                     GVRDVVVHVEPVETDREGDGAVP"
BASE COUNT          623 a          961 c          819 g          580 t
ORIGIN      
        1 tttattcttg tactccacga tctccgcttt gccccggata gtcaacgatc cacgcccgag
       61 tttgtacatg gcctcgatgc cgcgtgtccc gcacagtatg gcaccggtgg ggaaatccgg
      121 ccccttgaca aattgcatca gctcgtcaat cgtgcactcc ggattctcga tgtagtgggt
      181 gagcccgtcc acgagttcgc cgaggttatg cggcgggatg ttggtggcca tgccgaccgc
      241 aataccggtc gacccgttca agagcagatt cggcagccgg gcgggcagga cagtcggttc
      301 ctgaaactcc tcattgtagt tgggtttgaa atccaccgtc tctttttcca gatcatccag
      361 catctcctcc gccagacgat ccatgcgcac ctccgtgtac cgcatggctg ccgccggatc
      421 cccgtcgatg gaaccgaaat tcccctgccc gtccaccagc ggatagcgca tcgagaattc
      481 ctgcgccatg cgcacgatcg tgtcgtacac cgcctgatcg ccgtggggat ggtatttacc
      541 gatgacatcg cccaccacac gtgcggattt cttgtatggc ttgttcgatg tgttccccag
      601 ttcgttcatc gcgtacaata cccgacggtg caccggcttc agcccgtcac gcgcgtccgg
      661 caaagcgcgc ccgatgatta cgctcatgga atagtcgata tacgaggaac tcatctcgtc
      721 ttcgatattg atgttttgca acccttggtc ggtctgctgg tccggctgaa tcccgtcgct
      781 catgatgact ccgcttttga acagtgaaag tggggctcag tataccacat cagccgaaaa
      841 aaaaacacct tttcccgaat cggatcctgt ggtattttac ggtatgtgag aagcgattga
      901 accctttgtt gaggagcaaa ccgaaatgga taaccggacc ccctgcccga tctgccgcac
      961 cccgaacatt tcgtatgcca gtgccggagc caacaccagt gaaatcaact gcccgcgttg
     1021 cggccgctat cagatccagg cgttcgctga aacccaactc cacaacagcc cgcccgcttt
     1081 tcccccggcc catctgctct ccggattgtg ccgcaacacc tgggaccatc tgggggagaa
     1141 agtgttgatc accgtggatt tgttcaagtc ctgggccgat ctggaacggg tcgccaaact
     1201 ccccgtaccg cgcgacaccg atgtggtgac caagggggaa tacttgctga agcacctgcg
     1261 ccgccgatcg aaaacactgg cggacagggt ttcgtttacg ccccatgaac tggcgtgcgg
     1321 gttctgctcc acgaaaaacg aactgctttt ctgcctgcgt tatctggcag accgggggtg
     1381 ggtggaggaa tgttcagccg ccaatcgcaa gggacctcct cgtgacgcga tcacgtttcg
     1441 cctgacgccc gccggatggg cggcattggc cgctccggcc actccggccc aaccacgtgc
     1501 cgtggtgtcg gtcatgctgg atcgcgaaga cgagtctctc tggacgcaag ggttcgccgc
     1561 cgcggcgcag gccgccggat atgtggcgga gcgggttgag aaccgtgaac agctcaacaa
     1621 aattaatgac gagttgatcg tgtcgctgcg ccgcgccgct ttcgtgatcg cggatctgac
     1681 cggacaggcc cccatgacct atttcgttgc ggggttcgcc ggtggactcg gtaagcccgt
     1741 cttctggact tgcgaggaaa gcgaagcccg cgacaaacgg ttgtggctgg aaacacgcca
     1801 gtcaatcatc accacctgga cgcgggacaa gccggaggtg ttcgccgcgc gtctcgcgca
     1861 acgtatcgaa gccaccatcc ccggagaatg agcgcatgcg caaacggact tagggaacgg
     1921 ctccgtcccc ttcgcgatcc gtttcgacgg gctcgacatg caccacgaca tcccgcaccc
     1981 ccgacttggc cgccatgatc gcgtcccgta cctgatgccc gaccgcatgg ccctcgcgca
     2041 ccgataggtc gccgggaact tgaatatgca cctccacgaa gtaatcggtc ccgctcttac
     2101 ggatccggca cgcgctcacc gagtccacct gaggcacgcc gcgggcctcc cccatcaccc
     2161 ggaatcgcat ctcatcgccg acatccccgt cgagcatctc atggaacgcc ttccgtagaa
     2221 tgcgcacggc gttgaatacg atgatcccgc acgccagcag cgcggcccaa tcatccgcct
     2281 gggcaaaacg ggggccaccc agcagcgcga ggctgatccc cacggcagcc gccgcagagg
     2341 tcagggcgtc cgaacggtga tgccaagcgt cgccctccag tgcgagactg tccgtcgcac
     2401 gaccggcccg tagcacggcc cgcgagacgg cttctttgac caacacaacc attagcagta
     2461 ccggcaacgt gtaccacgca ggcgaagcgt gcggcgtcag cagttcgcgc actgcctgaa
     2521 aagcgatcag cagggccact gccagtaagg aaaagccgct gaacacgccg gccagagatt
     2581 cgattttccc gtgcccgtac gggtgacagg catcggccgg ccggagggaa aaatgaaacc
     2641 cgagccaagt gacggatgat gtgatgatgt cccctaagga ttccaccccg tcagcgatca
     2701 gcgcatagca gttccccatc agccccaccg ttatcttgac cagcatcagg ctgatgttga
     2761 cgcccagcgt caaaaaaccg agccaccgaa tggatttcga ttgcgacata tcggaacgat
     2821 cacataaaag ccccataccc gtcaatccaa tccgatcgta aagaataacg caggggaaaa
     2881 agtgctacac gcaccttctc ttctcactgc gcgtagactt cgagttcatc ggaaacccac
     2941 tcggtcatcc ggttctcgta gatcacgggg ccgccccatt tgc
//
LOCUS       DAOSRB010000285         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_30350_length_2951_cov_5.802141, whole genome shotgun sequence.
ACCESSION   DAOSRB010000285 DAOSRB010000000
VERSION     DAOSRB010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30350_length_2951_cov_5.802141"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PLZ74_10835"
     CDS             complement(<1..1399)
                     /locus_tag="PLZ74_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98854.1"
                     /translation="MKRRDFIKTTAAGVGGLTVLGARGQGVAASKKPLRVALIGCGDR
                     GVGQLLPECCKERVVALADPDPQRITAALGQVRKTLPSADIGAIKTFSDYRKLFDVMG
                     KELDAVVIATPNHQHTPPALMAIRRGIHVYVEKPLTHTIHEARLLRDEAKKHGVATQL
                     GNHGHSNEGCRRLCEYIWGGAIGQVREVYSWTQRCNGLLDGDHSPPLPLPQGFDWDAW
                     IGPAPFREFHADLHPHNWHLWRDFGNGSIGNMGCHILDPAFWALKLQAPEAVEVEEMR
                     GGASKVWSITTRIRFDFPARGEMAPVKVYWYDGLAQDQEYSERTVQRRWRCVNKREWQ
                     YVPPLVLELEKKHGRNFGTNGSLFVGDKGIMTIGEFGDGCRIVPEEAHRAYPVPDKIL
                     PRVKGTHQEDFFRACRGGAPACSNFEYSAPLAEIALLGNIAMIAGCGRRVEWDGDAMR
                     CTNMPELNAYVKTTYR"
     assembly_gap    1442..1541
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1579..>2951)
                     /locus_tag="PLZ74_10840"
     CDS             complement(1579..>2951)
                     /locus_tag="PLZ74_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98855.1"
                     /translation="VMGARGQDTAAAPDKPLRVAIIGCGDRGCHAILPNVMTSGMAEM
                     VAFVDPDARAIARARGRLKKYRPEANVESVAAFADYRDFYKQMAGGVDAVFVATPNHQ
                     HCLPALLAFRNGIHAFVEKPMANTIEEGRRMLAEARANRVVTQMGQHGHSNEGARRLC
                     EYVWAGAIGQVTEVYCWCDRLNAREQPLSDDSACPKELDWDKWIGPAAWRGFNSGLHP
                     VGWYSWRRFGSATIGNMGNHVIDPVFWALKLGSPESVQLVDFRPGAEGSWGLRDHIVW
                     KFPKRGDLAPVEMHWFDGLKGGLHWDDSTWTKGTYKYIVKKEHQNLPPKLVEIEKKYD
                     VDLGRCASVLIGEKGILCISVFGESLRFVPDSLRSTIPPPPKTIPRIRGSHQVDFMRA
                     CLGGPAACANFDYSQPLNEIVLLGVAAIEEGSGRRLDWDGKAGRFKDAVANKVLSVPS
                     RAGWEA"
BASE COUNT          554 a          984 c          770 g          543 t
ORIGIN      
        1 ctcgataagt cgtcttcaca tacgcgttca actccggcat gttcgtgcaa cgcatggcat
       61 caccgtccca ttccacacgc cgaccgcacc ccgcgatcat cgcgatgttg ccgagcagag
      121 cgatttcagc cagcggcgcg gaatactcga aattcgaaca ggccggcgca ccgccgcggc
      181 atgcgcggaa gaagtcttcc tgatgcgtgc ctttgacacg cggcagaatc ttgtcgggaa
      241 ccggataagc ccggtgcgcc tcctccggca caatgcggca gccgtcgccg aactcgccga
      301 tcgtcatgat ccccttgtcg cccacgaaca gcgagccgtt ggtgccgaaa ttccggccgt
      361 gcttcttttc cagttccagc accaacgggg gcacgtactg ccattcgcgc ttgttgacgc
      421 accgccagcg ccgctgcacc gtccgctcgc tgtactcctg atcctgggcc aaaccgtcat
      481 accagtaaac cttgaccggc gccatctcgc cacgggccgg aaaatcgaat cggatacggg
      541 tcgtgatgga ccagactttg cttgcccctc cgcgcatctc ctcgacctcc accgcctccg
      601 gcgcttgcag cttgagcgcc cagaacgcgg gatccagaat gtggcacccc atgttgccga
      661 tcgacccgtt gccgaaatcg cgccagaggt gccagttgtg cggatgcagg tccgcgtgga
      721 actcgcggaa tggagccggg ccgatccacg cgtcccaatc aaaaccctgc ggcagaggca
      781 aaggcggcga atggtctccg tcaagcaacc cgttgcagcg ctgggtccag ctatacacct
      841 cgcgcacctg accgatcgcg ccgccccaga tgtattcgca gagtcggcgg cacccttcgt
      901 tcgaatggcc gtggttgccc aactgcgtgg ccacaccgtg ttttttcgcc tcgtcccgca
      961 acagccgcgc ctcgtggatc gtgtgcgtca acggcttctc gacatacacg tgaatcccgc
     1021 gacgaatcgc catcagcgcc ggcggcgtgt gctggtggtt gggcgtggcg atgaccaccg
     1081 cgtccagttc cttacccatc acgtcgaaca gcttacggta gtcggaaaaa gttttgatcg
     1141 cgccgatatc ggcagaaggc agggttttac gcacctgccc caacgcagcc gtgatgcgct
     1201 ggggatcagg atccgccaac gcgacgaccc gctctttgca acattcgggc agcaactgcc
     1261 ctacaccgcg gtcaccgcag ccgatcaacg ccacgcgcaa gggctttttc gatgccgcca
     1321 cgccctgacc gcgcgcgccc aataccgtca gcccgcctac cccggccgct gttgttttga
     1381 taaaatcacg tcttttcatt gttcaactcc tcattcccaa atctcaactc ctaaatccta
     1441 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naactcctaa atcctaaatc
     1561 ctaacttcta cctcccaatc acgcctccca tcccgcgcgg ctcggcacac tcaaaacctt
     1621 gttcgctacg gcatccttga accgtccagc cttgccgtcc caatccagcc gtctgccgga
     1681 accctcctcg atagccgcga caccaagcag cacgatttca ttgagcggct gcgagtagtc
     1741 aaagttcgcg caagccgccg gaccgcccaa gcacgcgcgc atgaaatcca cctgatgcga
     1801 accgcggatg cgcgggatcg tcttgggcgg cggcgggatc gtcgaacgga ggctatccgg
     1861 cacaaaccgc aggctctcac cgaacacact gatgcagagg atacccttct cgccgatcag
     1921 aaccgaagcg caacggccaa ggtcgacatc atacttcttt tcgatctcca ccagtttcgg
     1981 cggcaggttc tggtgctcct tcttgacaat gtatttgtat gtgcccttcg tccatgtgct
     2041 gtcatcccag tgcagaccgc ccttgagccc atcaaaccag tgcatctcca caggcgcgag
     2101 gtcaccgcgt ttcgggaact tccagacaat gtggtcgcga aggccccacg acccctctgc
     2161 gcccggacgg aaatccacaa gctggacact ctccggactg ccgagtttca acgcccagaa
     2221 caccggatcg atgacgtggt tgcccatgtt gccgatggtc gcgctgccaa acctgcgcca
     2281 cgaataccag ccgacaggat gcaggccgct gttgaatccg cgccacgccg ccggtccgat
     2341 ccatttgtcc cagtccaact cttttggaca ggccgagtcg tccgaaagcg gctgttcgcg
     2401 cgcattgagc cggtcgcacc agcagtagac ttcggtgacc tggccgatgg cacccgccca
     2461 gacgtattcg cagagtcggc gcgcgccttc gttggaatga ccgtgctgcc ccatttgcgt
     2521 gaccacacgg ttggcacgcg cctcggcaag catccggcgt ccctcctcaa tcgtgttggc
     2581 catgggtttc tcaacaaagg catgaatgcc gttgcggaaa gcgaggagcg cgggcaggca
     2641 gtgctgatgg ttgggcgtcg cgacgaagac cgcgtccaca ccgcctgcca tctgtttgta
     2701 aaaatcgcgg taatcggcga acgcggcgac ggattccacg ttcgcttcag gccggtattt
     2761 ctttaagcgt ccccgtgcgc gggcaatggc gcgcgcatcg gggtcaacga acgccaccat
     2821 ctccgccatg cccgatgtca tgacgttcgg cagaatcgcg tgacagccgc ggtccccgca
     2881 accgatgatc gccacacgta acggtttgtc aggcgccgcc gccgtgtcct gaccacgcgc
     2941 ccccatgact g
//
LOCUS       DAOSRB010000286         2911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_30919_length_2911_cov_6.739146, whole genome shotgun sequence.
ACCESSION   DAOSRB010000286 DAOSRB010000000
VERSION     DAOSRB010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30919_length_2911_cov_6.739146"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..981)
                     /gene="rdgB"
                     /locus_tag="PLZ74_10845"
     CDS             complement(370..981)
                     /gene="rdgB"
                     /locus_tag="PLZ74_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOR98856.1"
                     /translation="MKGVIATRNHHKLQEIRAILSVPSWEWVALDEVPGMPDEVVEDA
                     ATFEGNALKKAREVCQVSGLWTLADDSGLEVAALQQAPGVFSARYAGESCDSRANNAK
                     LLRELAGVTDRRARFRCVIALCAPDGREWTVEGCCAGRIVESERGVNGFGYDPLFVPD
                     GYALTFAELDSVTKNRISHRGRALRLAAREWSESVLEQVKPAT"
     gene            complement(994..1587)
                     /locus_tag="PLZ74_10850"
     CDS             complement(994..1587)
                     /locus_tag="PLZ74_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR98857.1"
                     /translation="MTPAYTEARKACFTVLDFETTGSVPGYAVEPWQVGLVRVRGGRV
                     CPDEAYESLLRIGDRPFNPRAPGRHAMLREVLRDAPALAEVWPDLSGRISGVPLVAHH
                     VGTERSVLRRFAPLHPLGPWVDTLVLVRWAYPSLRSKALADVVDALGLSGRVRELCPD
                     RDAHDALFDAFACAAVLEHLLGLPGWEQITVQALAGR"
     gene            complement(1709..2539)
                     /locus_tag="PLZ74_10855"
     CDS             complement(1709..2539)
                     /locus_tag="PLZ74_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR98858.1"
                     /translation="MRQRFWIIMGLLAAGGAVALGLCLPRFFEGSMPQVPGEDVLHLV
                     LGDARQELSMALLAKADEYFHGGVNDVACGHGMTSVEGAVPDHDPAHGESCPEHAHAH
                     EAVRWRDPWAWINGRVHVQEHRHLCNDQATELLPWIWAACRASPQNAQAYQTGAYVLA
                     RMTGHPEQGVRLLEEGHRRNPEDAGIVFSLGEMWLTHLHDAARAESWFAAVLPLCRPA
                     EGAVGENDRLLWVRTLFYLGYLAKQRGDLARARACLAEAEAVFPEHASVKDLRRLLQQ
                     "
     gene            complement(2547..>2911)
                     /locus_tag="PLZ74_10860"
     CDS             complement(2547..>2911)
                     /locus_tag="PLZ74_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98859.1"
                     /translation="QAFLLHIGFLIVVTALAMLGSLMMTPSANLTLCGLLIVGMLVFG
                     QRLPSLIAAQPYPGKAFLMLVHALGPHVEFFDLRQRVIHGWSRVGWDVCAVVLLYAIG
                     YAAACLGLAGWLFCRKKV"
BASE COUNT          646 a         1017 c          784 g          464 t
ORIGIN      
        1 tgaacgggta gatttccagc aaagccccgt aaacattggg ttttacggtg cgcccgacgc
       61 tctttttgaa ctcttcgtcc cagcccttcg tcgcaagcgc aatcgacgga gttttggaga
      121 ccggtgcgta tgcgggacta aggcgtccct gtcgatcgtc gcgggtcgaa tgtcgcgggt
      181 cgcgggtcga cgttcccgat cccgatagcg atcccgaccc cgatttggat gaaaccctga
      241 aaccatgaaa ccatgaaacc agaaaccttg aaacccgatc ccgatcccga tagcgatccc
      301 gaccccgatt tggatgaaac cttgaaacca aagagagttg aaggttcaac tgctcttttt
      361 aggctgaagt caggtcgcgg gcttgacctg ctctaaaacc gattcgctcc attcgcgggc
      421 cgccaaccgg agcgcgcggc cgcggtgtga aatccggttc tttgtgacac tgtccagttc
      481 ggcaaaggtg agcgcatacc cgtccggcac gaacagcggg tcatagccga acccgttgac
      541 accgcgctct gactccacga tgcggccggc acaacatccc tccacagtcc actcccgccc
      601 gtccggtgcg cacagcgcga tgacgcagcg gaatcgggcg cgccggtccg taacgccagc
      661 cagttcccgc aacagcttgg cattgttggc gcgggagtca caggattcac cggcatagcg
      721 tgctgaaaag acacccggcg cctgttgaag cgcagccacc tccagaccgg aatcgtccgc
      781 cagcgtccac aagcccgaga cctggcagac ctcacgcgct tttttcagcg cattgccctc
      841 aaacgtggcc gcatcctcaa ccacctcgtc cggcataccg ggcacctcat ccaacgctac
      901 ccactcccat gagggaacag acagaatcgc gcggatctcc tgaagtttat gatggttccg
      961 tgttgcgatc acccctttca tcgtcttccc ccttcatctg cccgcaaggg cctgaaccgt
     1021 gatctgttcc cagccgggca acccgagcag gtgttcgagc accgcagcac aggcgaacgc
     1081 gtcaaacaac gcgtcatgcg cgtcacggtc gggacacagt tcccggaccc gtccggaaag
     1141 cccgagggca tcgaccacat cggccaaggc ctttgatctc agcgacggat atgcccagcg
     1201 caccagcacc aaggtatcca cccacggccc caacgggtgc agcggcgcaa agcgcctcag
     1261 cacactgcgt tccgtaccga cgtgatgcgc caccagcggc acgccggaga tcctgccgct
     1321 gagatccggc cacacctcag cgagcgcggg cgcatcacgc aaaacctcac gcaacatggc
     1381 atgccgaccc ggcgcgcgcg ggttgaaggg ccggtcgcca atccgcaaca ggctctcata
     1441 cgcctcatcc ggacaaaccc gcccaccgcg cacgcgaacc agtcccacct gccaaggctc
     1501 gaccgcatac cccggcaccg aaccggtggt ttcaaaatcc aacacggtga agcaggcctt
     1561 ccgggcttct gtgtacgcgg gcgtcatgcg tcagcgcatg ttcccgagtt ccgcatgaat
     1621 gcacccgcac gcggcgagca acaccagcag cgcacacgcc acaacacatc cgaacccgat
     1681 tgtccgcatc gtcatccgca tcccctttct attgctgcaa caaacgccgc agatccttca
     1741 cactcgcatg ttccggaaaa accgcctcgg cctccgcgag acaggcccgc gcacgcgcaa
     1801 gatcaccccg ttgtttcgcg agatacccga gataaaaaag cgtacgaacc cagagaagcc
     1861 ggtcgttctc gccgaccgca ccctcagcgg gacggcaaag ggggagcact gcggcgaacc
     1921 aggattccgc gcgcgccgca tcgtgcaggt gtgtaagcca catctcgccg agcgagaaga
     1981 cgatacccgc atcctcgggg ttacggcggt ggccctcttc caacaggcgc acgccctgct
     2041 cggggtgacc ggtcatgcgg gccagcacat aggcaccggt ctgatacgcc tgagcattct
     2101 gcggcgaagc acggcaggcg gcccagatcc acggcagaag ttccgtcgcc tgatcgttgc
     2161 aaagatggcg gtgttcctgc acatggacac gtccgttgat ccacgcccac ggatcacgcc
     2221 agcgcaccgc ttcatgcgca tgagcgtgtt cggggcacga ctccccgtgt gcgggatcat
     2281 gatccggaac cgctccctcg acagaggtca tgccgtgccc gcacgcgaca tcgttcactc
     2341 cgccgtgaaa atactcgtcg gctttggcca gcagcgccat gctgagttcc tgacgggcat
     2401 cgcccaacac aaggtgcagg acatcctcac ccggcacctg cggcatcgac ccctcgaaaa
     2461 accggggaag acataaaccg agcgcgaccg ctcctcccgc agccagtaac cccatgatga
     2521 tccagaaacg ttgacgcatg gcccttttac actttcttcc tgcaaaacaa ccatcctgcc
     2581 aacccgagac aagccgcggc ataaccgatc gcgtacagca gcaccacggc acacacatcc
     2641 caccccacac gcgaccaccc gtggatcacg cgctggcgca aatcaaaaaa ctccacatgc
     2701 ggcccgaggg catgcaccag catcaggaac gccttgccgg gatagggttg cgccgcgatc
     2761 aaggacggca ggcgctgccc gaaaacgagc attcccacaa tcaacaaccc gcacagcgtc
     2821 agattggcgg agggggtcat catcagggaa cccagcatcg cgagtgccgt caccacgatc
     2881 agaaagccga tatgcagcag gaaagcctga a
//
LOCUS       DAOSRB010000287         2894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_31173_length_2894_cov_7.319126, whole genome shotgun sequence.
ACCESSION   DAOSRB010000287 DAOSRB010000000
VERSION     DAOSRB010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31173_length_2894_cov_7.319126"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="PLZ74_10865"
     CDS             <1..1696
                     /locus_tag="PLZ74_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98860.1"
                     /translation="ELERCSSAVTLSDMEVFVFPELMYSLVLANIMSPCLWRWREDPW
                     FKGIRKMKPYRRLLRLKQYIMDHYVFNLDLETWGLTHQHHELERFANFLAPEVIAQSN
                     ALFGYQGDTYYFDIDIRTHFGLDKYASEIIPYWKTETIEAMNAFQFREGYAAGAGECV
                     SLAALYAAALYVVAGIPLEKVFLMATPLHSQNFVEVDDGVLINNRRLVTKAMWFNGTQ
                     ISKQARRALENERVTIVSHPTGWIHMLYPRATIDAEAYGRFATGLRAYLKTPLTPEIL
                     GNFLRQNPQCRQCFVMRHPIHGVDRYLSLEQAFQFEQECPYKVTDATRDKLLAMIPME
                     TFAASCCPGKIVLNDIEAFVQERAIDLDRSEDLKALRTAIGDTCMSAAEMVEQLQRFC
                     HTEPRLPDAAGVSFTPPPPILLPVDLTREEVIARIEDLRQVNLTADLAFYAWRDLTRT
                     PYAPFVKAALERNPVSLAGAAEMSESDLASLLAAWPGDSIYEGPARLAQPDEVWNFRR
                     GDGLEKALLMANVLRHRGCGPLRLTSEEGRAVLSAGERTACAFASAKTPAEQVWNL"
     gene            2038..>2894
                     /locus_tag="PLZ74_10870"
     CDS             2038..>2894
                     /locus_tag="PLZ74_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOR98861.1"
                     /translation="MSKSNVILSTVAAVGILCLQVKAEERVDAPRWYVNPGVGMILPE
                     GNQAIDEGLNLNLRLGYDLSDHWSVETEASFAPNMTNNGRWTQKKQRIYGVGLDALYH
                     FDRYNRFDPFLSAGWAVYMADRNVFEEGSRSTLTGPRVGIGAMYHLTDNLSLRADGRA
                     LMSVDTCHEMQYTLDAGVVYRFGGSGGSSFGEGSSSVTNLPKDSDGDGLTDDEEAKLG
                     TDPFKADTDGDGLTDFEEVRTYKTDPLNPDTDFDGLSDGDEVKKYKTNPLDRDTDKGG
                     VADGHEVIE"
BASE COUNT          568 a          816 c          940 g          570 t
ORIGIN      
        1 ggagttggag cggtgttcgt cggccgtcac gctgtccgac atggaggttt tcgttttccc
       61 ggagttgatg tacagcctgg tgctggcgaa catcatgtcg ccatgtctct ggcggtggcg
      121 cgaggatccg tggttcaagg ggatccgcaa gatgaaaccc taccgcaggc tgctgcgcct
      181 gaagcagtac atcatggatc actacgtgtt caatctcgat ttggagacat gggggttgac
      241 ccatcagcac cacgagcttg agcggttcgc aaacttcctc gcacccgagg tgatcgcgca
      301 gtccaacgcg ctgttcggtt atcagggcga cacctactat ttcgatatcg atatccgtac
      361 ccatttcggt ttggacaaat acgcctcgga gatcatcccc tactggaaga ctgagacgat
      421 cgaggccatg aacgcgtttc agttccgtga ggggtacgcc gccggggcgg gcgagtgtgt
      481 ctcgctggct gcgctctacg ccgcggcgct gtacgtggtg gcgggtattc cattggagaa
      541 ggtgttcctg atggccacgc ctctgcactc ccagaatttc gtggaggtgg acgacggcgt
      601 gctcatcaac aaccggcggc tggtgacgaa ggcgatgtgg ttcaacggca cccagatttc
      661 gaaacaggcg cgccgcgcgc tggagaacga gcgggtgacg attgtttcgc accccacggg
      721 atggatccac atgctctacc cgcgcgccac gatcgacgcc gaggcgtacg ggcggttcgc
      781 caccggattg cgcgcgtacc tgaagacgcc gctgacgccg gagattctgg gcaacttcct
      841 gcggcagaac ccgcagtgcc ggcagtgctt cgtgatgcgg catccaattc acggagtgga
      901 tcgctatctg tcgctggagc aggcttttca atttgagcag gagtgcccgt acaaggtcac
      961 ggacgccacg cgcgacaaac ttctggccat gatcccgatg gagacgttcg cagcctcgtg
     1021 ctgtccgggc aagatcgtgc tgaacgacat cgaggcgttc gtgcaggagc gcgccattga
     1081 tctggaccgg tccgaagact tgaaagcgct gcgcaccgcc attggcgaca cctgcatgag
     1141 cgcggccgag atggtcgaac agttgcagcg tttctgccac acggaaccac ggctgccgga
     1201 tgccgcgggc gtgtcgttta cacccccccc gccgatcttg ctcccggtgg acctgacgcg
     1261 cgaagaggtg atcgcccgga tagaggatct gcgtcaggtc aacctcaccg ccgatctggc
     1321 cttttacgcc tggcgcgacc tgacccgtac gccctatgcc ccgtttgtca aggccgcgct
     1381 ggagcggaac ccggtgagtc tggcgggtgc cgccgagatg tcggagagcg atctggcctc
     1441 actgctcgcc gcatggcccg gagactccat ctatgagggg cccgcgcggc ttgcgcaacc
     1501 ggatgaggtg tggaacttcc ggcgcgggga cggtctggaa aaagccctgc tgatggccaa
     1561 cgtgctgcgc caccggggat gcggccccct gcgcctgacc tcggaagaag ggcgggccgt
     1621 gctgagtgcc ggagagcgta ccgcctgcgc tttcgcatca gcgaaaacgc ccgccgaaca
     1681 ggtgtggaac ctctgacgcg caggttggca cgcggtttgc atagtaaaaa gcggactggg
     1741 cacgcgatgc gcgcgtccag tgcttcggca ccggaaacca accctctcca gtgcaacaga
     1801 acgccaggtt cggacattag gggtgcgcac agaagcacgc agtttcttgc gaagcttgtg
     1861 tccgcaagga tgcgggcccg ccacggggga acgtggcggg ccgtattttt tcaggcctcc
     1921 ggttgcggag cggtatgggc ctaaccttaa aaaaatgtca tgtaagccga ttcttgttgc
     1981 atcgcgcgtc cgtatccgct aaacttattt catctttggg ctataaggag gaatcaaatg
     2041 agcaaatcaa atgtgatctt gtctacggtt gcggccgtcg ggatcctgtg tttacaggtg
     2101 aaggccgagg agcgggtgga tgcgccccgc tggtatgtca atcccggagt cggaatgatt
     2161 ctccctgagg gaaatcaggc gattgacgag ggattaaatc tcaacctgcg tctcggttac
     2221 gatctttctg accactggtc ggtggagaca gaagcgtctt tcgcgcccaa catgaccaac
     2281 aacgggcgtt ggacgcagaa aaagcaacgc atttacggcg tggggctgga tgcgctgtat
     2341 catttcgatc ggtataaccg attcgacccg ttcctttctg cagggtgggc cgtgtacatg
     2401 gcggaccgca atgtgtttga ggaggggagt cgttcgaccc tgacggggcc gcgggttggc
     2461 attggcgcca tgtatcatct gaccgacaac ctctccctcc gggctgacgg cagggcactg
     2521 atgtcggttg atacgtgcca tgagatgcag tacacgttgg atgccggagt ggtttaccgt
     2581 ttcggcggca gcggcggcag cagtttcggt gagggcagct cgtcggtgac caacctgccc
     2641 aaggattcgg acggcgacgg gttgaccgac gacgaggagg cgaaactggg aaccgacccc
     2701 ttcaaggcgg atacggatgg cgacggactg accgattttg aggaggtccg cacctacaag
     2761 accgacccgc tcaatcccga tacggacttc gacgggttgt cggacgggga tgaggtcaaa
     2821 aagtacaaga ccaacccgct ggaccgtgat accgacaagg gtggcgtggc ggacggacac
     2881 gaggtgatcg agga
//
LOCUS       DAOSRB010000288         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_31412_length_2876_cov_5.529954, whole genome shotgun sequence.
ACCESSION   DAOSRB010000288 DAOSRB010000000
VERSION     DAOSRB010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31412_length_2876_cov_5.529954"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="PLZ74_10875"
     CDS             complement(<1..94)
                     /locus_tag="PLZ74_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOR98862.1"
                     /translation="MRDVWIAFGLTLFAGLATGIGSMIAFLAKRT"
     gene            complement(119..616)
                     /locus_tag="PLZ74_10880"
     CDS             complement(119..616)
                     /locus_tag="PLZ74_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOR98863.1"
                     /translation="MRAIQADITTLQVDAIVNAANTTLLGGGGVDGAIHRAAGPDLLD
                     ECRSLGGCAVGDAKITRGYKLPAKHVIHTVGPVWRGGNTGEEQHLANCYRRSLEIAQE
                     HGVKSIAFPAISTGVYGYPIEDATRVAAATVRAYLQESQSAMDVIFCCFSQRDLAVYD
                     KELAR"
     gene            1075..>2876
                     /locus_tag="PLZ74_10885"
     CDS             1075..>2876
                     /locus_tag="PLZ74_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98864.1"
                     /translation="MVGAFCAVLVCGWGSAQDTTVWTNPSGGVWNDAGNWNNGVPDGN
                     KASLTLNTASYIVDIESAPVPAIMNLTAQNTASQTTLLNVNTGTLAVTKGAILIETGA
                     EVCIKSNACLSFTGGSTTDFQGFFKTRSGGKLRVDGGSVCFTGVTFGSKGAIVGFCGA
                     GSAVVTSGVFQLSGSGNPPLHIARGENAKGYFEAAGSSRVDVAQLTIGMYGQPNSPAA
                     GRLEVKGDSVFTVGNSATVNVPLYNAPSGINNTGIVEVSENALLTCLGSYFDLGYATG
                     ATTNLGVLRVTGSGRFAAPNATLRCGNTDAGSAIFEVGDNGVCEIKNLYLNGNTTATL
                     NGNGRCFVTGSSAPRIGNGAACSNVVLQILDQALFAVTNATLYCGYDNSTAEIVVGSN
                     AVCMAQTSRFYGNSSLTVCGDGCYTGMVADTLNDSDYIGYNAGKVVLNVSGNGRFLTK
                     RNLNIGYFGAEGEVNVSGNGRLSTGCDFVIGYVNSSAASKAAVNLSGNGVIAPSVSGR
                     GLHMAVVGNSSTSVISNGMVQAWLNISDGLFDMTKDMWNADSFYEEGIIAGEIWKNNQ
                     GAAEAWINLSGGTVTNAGKMTAGMGLGATGVVTQ"
BASE COUNT          602 a          762 c          914 g          598 t
ORIGIN      
        1 cggtacgctt ggcgagaaag gcgatcatgc taccgatacc ggttgccagc cccgcgaaca
       61 atgtcaatcc gaaagcgatc caaacgtctc gcatgccatc ctctcttttc ccaaagcgct
      121 atcgcgccaa ttccttatcg taaaccgcga ggtcgcgctg agagaagcag cagaatatca
      181 catccattgc cgactggctc tcctgcagat aggcgcgcac ggttgccgcg gcgactcgtg
      241 tcgcgtcttc aatgggatag ccgtagacgc cggtgctgat tgccgggaaa gcaatcgatt
      301 tcacgccatg ctcttgcgcg atttctaggg atcggcggta acagttggcg aggtgttgct
      361 cctctcccgt gtttccaccg cgccagacgg ggccgacggt gtggatgacg tgtttcgcag
      421 gtagcttgta tccgcgcgtg atcttggcgt cgccaaccgc gcaccccccg agcgaccggc
      481 actcgtccaa gagatcggga ccggccgcgc gatggatggc gccgtcaaca ccacccccgc
      541 ccaatagcgt cgtgtttgcc gcattgacga tcgcgtcgac ctgtagtgtc gtgatgtctg
      601 cctgtatcgc tctcatgtgc tcctcgcggt taacagtgtt atcgtgcccg tcttaaaacc
      661 cgtacataac gaaaaccgta ttcattctga ctgtcgaggt caagcgtgca cttgcttgac
      721 aaagggccgc gcgcggaaac cgtggcaagc gtctgcttgt ccggacgagc ggacgttcgt
      781 ttcgtccatc ttgatttcat gaaattttat gtgtttcatg gtaagaacgc taaagtgcgg
      841 atcatgcagg gtacggggag gacagcggga acacgtccgt caagtccacc tcgaattcta
      901 ggtttgattt tccgattcgg acgtagcata gtgttgtgtg ggaaggggct gatacggagg
      961 attgtccgaa agaccctgga aggtgcggtc agacaggctc ggtttgtcag ggttcattga
     1021 ggcgttgaga tcatgaaagg acaagcgatg aagatacggg atagaagggg ctggatggta
     1081 ggggcgtttt gcgctgtgct ggtgtgcgga tggggttcgg ctcaggatac gacggtgtgg
     1141 acaaacccgt ccggcggcgt ttggaatgac gcagggaact ggaacaacgg ggtgccggat
     1201 ggtaacaagg ccagtctgac gctgaatacc gcgtcatata ttgtggacat cgagagtgcg
     1261 ccggtgcccg cgatcatgaa tctgacggcg cagaatacgg cttcgcagac cacgctcctg
     1321 aatgtgaaca ccggaacgct ggccgtcacc aagggcgcca ttctcatcga aaccggagcc
     1381 gaggtttgca ttaagagcaa tgcctgcctg agcttcacag gcggttcgac aacggacttc
     1441 caaggctttt ttaaaacccg gagtggcggg aaactacggg tggacggggg ctcggtctgt
     1501 ttcacgggtg tgacgttcgg ttcgaaagga gcgatcgtcg gattctgtgg cgcgggctcc
     1561 gctgtcgtca cctcgggcgt attccagttg agcggcagcg ggaatccgcc gttgcatatc
     1621 gcccgcggcg aaaacgcgaa aggctatttc gaggccgcgg gttccagccg ggtcgatgtg
     1681 gcacaactaa ccatcggcat gtacggccaa ccgaactcgc ccgctgcggg cagactggag
     1741 gtgaagggcg attccgtctt tacggtgggg aattcagcaa cggttaacgt tcccctttac
     1801 aacgccccca gcggcatcaa taacaccggg atcgttgagg tgagcgaaaa cgcgctgctc
     1861 acctgtctgg ggagctattt cgatctcggg tacgccacag gcgcgacaac gaacttgggc
     1921 gtgctgcgtg ttacgggctc cggacgattc gcggccccca acgccaccct gcgttgcggc
     1981 aacacggatg cggggtcggc aattttcgag gttggcgaca acggcgtgtg tgagatcaaa
     2041 aacctctatc tgaacggcaa tacgacggcg accctgaacg ggaacgggcg gtgttttgtg
     2101 accgggagca gtgcgccccg gattggaaac ggcgcggcat gctcgaatgt ggtactccag
     2161 attctggatc aggcgctgtt tgcggtgacg aacgccacgc tctattgcgg atacgacaac
     2221 agtacagcgg agatcgtcgt cggttcaaat gcggtgtgta tggcccaaac cagccgcttt
     2281 tacgggaact cctcgctgac cgtttgcggt gacggttgtt atacgggcat ggtcgcggac
     2341 acgcttaacg acagtgatta cattgggtac aatgccggaa aggtggtgct caacgtttcg
     2401 ggcaacggcc ggtttctgac gaaacgcaac ctcaacatcg gttattttgg cgcggagggc
     2461 gaggtgaatg tttcaggtaa cgggcgtctc tctacaggtt gcgatttcgt gatcggttac
     2521 gtgaacagca gcgctgcgtc aaaagccgca gtcaacctct cgggcaacgg cgtgatcgcg
     2581 ccctcagtct cggggcgcgg actccacatg gcggtggtgg gaaactccag cacaagcgtc
     2641 atctccaacg ggatggtgca agcctggctg aacatttccg acgggctgtt cgacatgacg
     2701 aaagatatgt ggaatgcgga ttccttttac gaggagggga tcatcgccgg ggaaatctgg
     2761 aagaacaacc aaggagcggc ggaagcgtgg atcaacctca gcggcggcac ggtcacgaac
     2821 gccggcaaga tgacggccgg catggggctg ggtgccacgg gtgtcgtgac ccagat
//
LOCUS       DAOSRB010000289         2873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_31465_length_2873_cov_6.587296, whole genome shotgun sequence.
ACCESSION   DAOSRB010000289 DAOSRB010000000
VERSION     DAOSRB010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31465_length_2873_cov_6.587296"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..885
                     /locus_tag="PLZ74_10890"
     CDS             328..885
                     /locus_tag="PLZ74_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOR98865.1"
                     /translation="MHRFPVPPRFRDKSPNGIALNHRIMVEDMPAWSPCLRSKTTIRT
                     SDTRYFTDPSIAAAAMGLGPGDLINDIQSLGFFFEALAVRDLRVYAEALGGAVSHFLD
                     RNGLECDAVVHLRNGSSGLVEVKLGGEALIEEGASTLNALSGLLDTTRMKAAAFKMVL
                     VAVGKYAYRRPDDNVIVCPIGCLKP"
     gene            1065..1790
                     /locus_tag="PLZ74_10895"
     CDS             1065..1790
                     /locus_tag="PLZ74_10895"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOR98866.1"
                     /translation="MKKLIEKLTKARCVAVFTGAGVSTLCGIPDFRGPQGLYKQPNAE
                     RIFDIEWFRRDPSVYYKGCMELVYGLRRFKPGPVHHALVHLERSGIVRGIITQNIDML
                     HQKAGSKTVYEVHGSPILHHCFGCGQEKTFDEICAMLEDTPVPRCGHCGEAFKPDITF
                     FGEMLPEQAFEQSVALARQADLMLVLGSSLTVHPVASIPPLTVRAGGELVIVNAQPTP
                     LDQIATLLYPDLNAFADAVLQVH"
     gene            2301..>2873
                     /locus_tag="PLZ74_10900"
     CDS             2301..>2873
                     /locus_tag="PLZ74_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR98867.1"
                     /translation="MKKSRRIGLLVLTLAGASLRAGERMPLWPEGKIPDFQDHQVGVM
                     TDAMWLPGTWGKPNPAFKPEEHRMPYIEWYGKAENPNGACIILISGGSYQNCCDVNLV
                     KKMWPNRLIELGYQCVNFVYRTPRPKGLPIYQSAWQDGQRAVRLVRSEAAKRGFDPER
                     IGLISMSAGGHLGVMMATSSLTPAYAKVDEL"
BASE COUNT          583 a          779 c          888 g          623 t
ORIGIN      
        1 ccgaatccat cgtgaacctc acgcggtcac tcgcggcgct cgccaatctc cgcgcatagc
       61 gcggagattg gagttaggaa ttaggagtta gcctgccgcg cggcttcgcc gcgaaaaaac
      121 cttggaatac gtttaggaca tgatttccga cagcttcgct tcggcggaga caatcctgaa
      181 aatcctgtaa tcctgtcaat ttataggcaa ggaatttttg ggaaccgcca aggtttttgg
      241 ggggcgtggc tccagccgca ggtcgctggt cgccggtcaa cctcttattg cgaccacgca
      301 gtggctcgca attttgagag taaaggcatg caccgctttc cggttccccc acgcttccgc
      361 gacaaatcgc cgaacggaat cgcgctaaat caccgaatta tggtcgaaga catgccggcg
      421 tggtcgcctt gtctgcggtc aaagacaacg atccgcactt cggacacgcg ctatttcacc
      481 gatccgtcca tcgccgccgc cgccatggga ttgggacccg gcgacttgat aaacgacatc
      541 cagtcactcg gttttttctt cgaggcactc gccgtgcgcg atttgcgcgt gtacgctgaa
      601 gcgctcggag gtgcggtcag ccactttctc gaccgaaacg ggctggagtg cgatgccgtg
      661 gtgcatttgc ggaacggctc ctccggtctt gtcgaagtga aacttggcgg cgaggctctc
      721 atcgaggaag gcgcttcgac actgaatgcg ctttcgggac tgctcgacac gacacggatg
      781 aaggcggcgg cattcaaaat ggttttggtg gccgtaggca agtacgcata tcggagaccc
      841 gatgacaacg tgatcgtctg tccgatcgga tgcctcaagc cctgaccccg gcaccgccta
      901 ttgaggtgat tgcgacaagg tgctctggtt ataggcaagg taaagggaaa cagccttgcc
      961 gcaacacata aatctgacta tgctctattc tagcgcctta ccggccgata aggccaataa
     1021 aaggttgttt ctcacacgaa gggcgcgaag gggaaagggt tgtcatgaaa aaactgatcg
     1081 agaaactgac gaaggcacgg tgcgtggcgg ttttcaccgg cgcgggcgtc tcaacgcttt
     1141 gcgggattcc ggattttcgc ggaccgcagg gactctataa acagccgaat gccgaacgta
     1201 tcttcgatat cgaatggttc cggcgcgatc cctcagttta ctacaagggc tgcatggaac
     1261 tggtgtacgg gctccggcgt ttcaagcccg gcccggtgca tcacgcgctg gtgcatctgg
     1321 agcgctcggg aatcgtccgg ggtatcatta cgcaaaacat cgacatgctg catcagaagg
     1381 ccgggtcgaa gaccgtgtac gaggtccatg gctctcccat cctgcaccac tgcttcgggt
     1441 gcgggcagga gaagaccttt gacgagatct gcgcgatgct ggaggacacg cctgtcccgc
     1501 ggtgcgggca ttgcggagaa gccttcaagc ctgacatcac gttcttcggc gagatgctgc
     1561 ctgagcaggc gtttgaacaa tcggtcgcgc tggcgcggca ggccgacctg atgcttgttc
     1621 tcggctcgtc gctgaccgtc cacccggtgg cttcgatccc gccgctgact gtccgggcgg
     1681 gaggcgaact ggtgatcgtc aatgcccagc cgacccctct ggatcaaatc gcaacgctgc
     1741 tctatcctga cctgaacgct ttcgccgatg ctgtgttgca agttcattag ttcgttggtg
     1801 cgttacggct taacaaggtt tgtacagtct tttgattgcg ggctccgccc gcgttggggg
     1861 ttaaggctgt gaggggttaa ggctgcaagg tgttaaggct gtgaggggtt aaggctgtga
     1921 gggaatatgc ggcgggtcgc atcaaacagc ctcacagtct cacagcctca actcctcact
     1981 gtctttgaga ttgcgggctc cgcccgcgtt agtgcggaat cgggatcgga atcgctatcg
     2041 ggatcgtccg cggacgcctc ggggatgcgt ccctgcccct ggcgcgtagc cgaggacgtc
     2101 cgaggaggcg gaacgctgaa cgtgaaacca gaaaccagaa atctcggcac tcttggcgat
     2161 tccagaaacc ctaaagcgtt gagttttact gtttataggt ttagtggttg actggtttca
     2221 tctccctcgt acaatgggtg gctatcactg gaggcggcgt gttcccggtc tgcccctgga
     2281 ggacaatgac ggagtcgatc atgaagaaat ctcgtcggat tgggttgctg gtgttgacgt
     2341 tggcgggtgc gtccctgcgt gcgggcgagc ggatgccgct atggccggaa gggaagattc
     2401 ccgatttcca agatcatcag gtcggggtga tgacggatgc catgtggctt ccggggacgt
     2461 gggggaaacc taacccggcg ttcaagccgg aggagcatcg tatgccgtac atcgaatggt
     2521 acggcaaggc agagaacccc aatggggcgt gcatcatcct catttcagga gggagttacc
     2581 aaaactgctg cgatgtgaat ctggtgaaaa agatgtggcc gaaccgtctc atcgaactcg
     2641 gctatcagtg tgtcaatttc gtctatcgga cgccgcgtcc gaagggactg ccgatctacc
     2701 agagcgcctg gcaggacgga cagcgcgccg tgcgtcttgt ccgttccgag gccgcaaagc
     2761 gcggtttcga tcccgaacgc atcggcctga tctcgatgtc cgcaggcggg catctcggtg
     2821 tgatgatggc gacgagttcc ctgacgccag cgtacgcgaa ggtggacgaa ttg
//
LOCUS       DAOSRB010000290         2864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_31600_length_2864_cov_12.008900, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000290 DAOSRB010000000
VERSION     DAOSRB010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31600_length_2864_cov_12.008900"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..304)
                     /locus_tag="PLZ74_10905"
     CDS             complement(86..304)
                     /locus_tag="PLZ74_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98868.1"
                     /translation="MPVADDFVESVMRCVLAVGPSRLITPTTFWHHAGHHESRQGEDH
                     RSPVKVDFPEPGAYAIEYVVTAASPRSR"
     gene            complement(309..1754)
                     /locus_tag="PLZ74_10910"
     CDS             complement(309..1754)
                     /locus_tag="PLZ74_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOR98869.1"
                     /translation="MMRMFSLPVVCVMFGFLSAHGANAFMQDARRSPEWLTRGVMYQI
                     QPRAFTPEGTLKAAEAKLPYLKDLGVTIVYLVPVMKMDESMDKAFWSPRQIKSGFNNP
                     KNQYRIADYFHVDPEYGSDRDLVDFSATAHRLGLKVILDLVYLHCGPAAPFLKEHPEF
                     TWWNDDGTPKKGPWRFPKLNFAEPRLRAYLMENMRYLIREYGADGFRCDVGDGIPLDF
                     WCDAHDMLDRETHGKAVLLCEGFTVCNQYKGFDADYGWFPGLSARTVRQSWTNRERQC
                     PVGSRFVNHYENQDIATDLRPRRERQWGQASVDQVLVWMFTLDGVPMLFMGNELADAD
                     ERHSMFGKTPMDWSQLDREPGKSRHALVKKLAGLRRTHTVFTDVNGKDGLTWLDTTAT
                     NAVTAFVRRNKKTTVLVVQNWSAETVTCDVSFKVDAAKTPSYLAPDETDRSVNGTVVP
                     EPIFAKDATFGDVTRFVLGPWGCWISEIRLK"
     gene            complement(1751..2791)
                     /locus_tag="PLZ74_10915"
     CDS             complement(1751..2791)
                     /locus_tag="PLZ74_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOR98870.1"
                     /translation="MVGALAAAVVLKGAYPLLVTPYTEKAELDCEVLIREAEYVDAQG
                     VGGVIWPTANEINVLDEFGEYESGLTALADRWAKGGMKSRLTAVCCGTNSAAALKRVE
                     LWDAIVSKRGISAAILARPPDDAKTDASMEAHYRLLAAKTSLPVIIQTFCKGSNPQPS
                     VDLLVRLSRDFPNTYGYFKEESNRSDVNDRIAAMMAAKPAVKGVFSGWGATQFLYQGP
                     RLGTTGIITQRPMYAGLLQKMLDLTAAGKTAADPELADTFGRYLMMVNLGEVFKSQKT
                     DGGSMRGPHLVVFKRLGVFRNTLTRTVDGNGRKSVKSFDLPPNAIAEIDARIKSAGLQ
                     KSSNSWKGKGVL"
BASE COUNT          602 a          885 c          783 g          594 t
ORIGIN      
        1 ggaagacgcc cgcgctcccg actgcgcaca cacaaaccac gcactaaact cgcatgcccc
       61 ctgtcaccaa ccgattacac gccatttatc tggagcgcgg agatgcggcg gtcaccacgt
      121 attcgatcgc gtaggcgccg ggttccggga aatcgacttt tactggagat ctatggtctt
      181 cgccttgtcg agattcatga tgaccggcgt gatgccagaa ggttgtcggg gtgatcaggc
      241 ggcttgggcc gaccgccaac acgcagcgca tcacggattc gacaaaatca tcggcaacag
      301 gcattttttc attttagcct tatttctgaa atccaacatc cccaaggccc cagtacaaac
      361 cgcgtcacat cgccgaacgt cgcatccttt gcgaagatcg gctcaggcac aacagtaccg
      421 ttaaccgaac gatccgtctc gtcaggagcg agataggacg gggtctttgc cgcatcgacc
      481 ttgaacgaga catcacatgt cacggtctcc gcagaccagt tctggacgac cagcacagtc
      541 gtctttttgt tccggcgcac gaaggctgtg accgcgttcg ttgccgtcgt gtcgagccac
      601 gtgagtccat ctttcccatt cacgtccgta aagacggtgt gtgtccgccg caggcccgca
      661 agcttcttca caagcgcgtg acgcgacttg ccgggttcgc ggtcgagctg agaccagtcc
      721 attggcgttt tgccgaacat cgaatgacgt tcgtcggcat cggcgagttc gttccccatg
      781 aagagcatcg gcacaccgtc gagcgtgaac atccacacga gcacctgatc gacggaggcc
      841 tgtccccact gccgttcacg gcgcggccgc aggtctgtcg caatatcctg gttttcataa
      901 tggttcacga aacgtgaacc aacgggacac tggcgctcac gatttgtcca tgactgccgg
      961 acggtgcgcg cggagagtcc ggggaaccag ccatagtccg cgtcaaagcc cttgtattgg
     1021 ttgcagacgg taaagccctc acaaaggagc acggccttgc cgtgcgtctc acggtctagc
     1081 atgtcgtgcg catcgcacca gaagtcgaga gggataccgt ctccaacatc gcagcgaaag
     1141 ccatccgctc cgtattcgcg gatgagatac cgcatgttct ccatcagata agcgcgtagc
     1201 ctgggttcgg cgaagttgag cttcgggaag cgccacggcc ccttcttcgg cgtcccgtca
     1261 tcgttccacc aagtgaattc ggggtgctct ttcaggaatg gcgcggcggg tccacaatgg
     1321 agatagacga gatcgagaat gaccttcagc cccagacggt gcgcagtcgc tgagaagtcc
     1381 acaagatccc ggtccgaacc gtattcggga tcgacatgga aatagtctgc gatacggtac
     1441 tggttcttcg ggttattgaa gccgctcttg atctggcgcg gactccagaa agccttgtcc
     1501 atcgactcgt ccatcttcat gacggggacg agatagacga tcgtgacacc gagatccttc
     1561 agatacggga gcttcgcctc ggcggccttg agcgttcctt ccggcgtgaa cgcccgcggt
     1621 tgaatctggt acatgacgcc gcgcgtcagc cactcgggcg aacgtcgcgc atcctgcatg
     1681 aacgcattcg cgccgtgcgc cgagaggaag ccgaacatca cacacacgac aggcaaactg
     1741 aacatccgca tcataacaca ccctttcctt tccaagagtt tgagctcttt tgaagtcccg
     1801 cgctcttgat gcgcgcatcg atctccgcaa tggcgtttgg agggagatca aaagacttta
     1861 cgcttttgcg tccattccca tcgacggtac gcgtgagcgt attgcggaat acgccgagcc
     1921 gcttgaacac cacgagatga ggcccccgca tcgagccgcc atccgttttc tgcgacttga
     1981 acacctcgcc gagattaacc atcatgaggt accgtccgaa ggtgtccgca agttcgggat
     2041 ctgcggcggt ttttcccgct gccgtcaggt cgagcatctt ctggagaagc cccgcgtaca
     2101 tggggcgctg ggtgatgatg ccggtcgtcc cgagtcgcgg tccctgatag agaaactgag
     2161 tcgcgcccca gccggaaaag acgcccttca cagcgggttt cgcggccatc atcgctgcga
     2221 tacggtcgtt gacatccgac cgattcgact cctccttgaa ataaccgtag gtgttcggaa
     2281 aatcccgaga aagccgcacc agcagatcca ccgatggctg cggattcgac cctttgcaga
     2341 aagtctgaat aatcacagga agcgaggtct tcgccgcgag caaacgatag tgcgcctcca
     2401 ttgaggcatc ggttttggcg tcgtccggag gacgtgccag aattgccgcc gaaatgccgc
     2461 gcttgctcac gatggcgtcc caaagctcaa cccgtttcag cgccgcggcg gagttggttc
     2521 cgcaacagac cgccgtgagc cgggatttca tcccgccctt tgcccagcga tcggcaagcg
     2581 ccgtcagtcc cgactcgtac tcgccaaact cgtcaagaac gttgatttcg tttgccgtgg
     2641 gccagattac gccgcccacg ccctgcgcgt cgacatattc cgcctctcgg atcaggactt
     2701 cgcaatccag ttccgctttt tccgtgtagg gcgtaaccaa cagcggatac gcccctttca
     2761 acactaccgc agcagctaaa gctcctacca tgtctttttc ctaatctcag gaggctcttg
     2821 caaaacccct cgtggcatgg gcgtcccgcc catgaaacac gggc
//
LOCUS       DAOSRB010000291         2854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_31767_length_2854_cov_6.021079, whole genome shotgun sequence.
ACCESSION   DAOSRB010000291 DAOSRB010000000
VERSION     DAOSRB010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31767_length_2854_cov_6.021079"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1009
                     /locus_tag="PLZ74_10920"
     CDS             62..1009
                     /locus_tag="PLZ74_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOR98871.1"
                     /translation="MGWRGKTLGGSIGSFFGPWGALAGAAAGHFLVDRKKTRRAVNSP
                     LRLLAVTAAALREVAEADGRYTLREDRVIRAVLDGIRGEIGAPLGPHELAYLIDDAAR
                     IDQGLLRLAALARDMPGLGHAAVVWLWRVAVSEAGETSDGAARIARFAQQAGLSSGEA
                     RFCALPYSRTACADPSGVARRADCDTLGVPYDADPVQIKAAYRALSLTYHPDKHAGLD
                     PAIRALTAAKFTEIKAAYDRLRGGSRAGLLVRLAGADRLVPYADGVTAACFGCGRSVR
                     LHEESDPLTLRCDACQMLFAMEPERLAAMENSRGASPFS"
     gene            1135..1791
                     /locus_tag="PLZ74_10925"
     CDS             1135..1791
                     /locus_tag="PLZ74_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98872.1"
                     /translation="MKTRQMLDEGQWIEIGHGSRRKCFRVGNEPVCVKFYRLPSEYTH
                     KTTWGVRLHIGLARFRPHFNVNCQEWRYHRALRERLPPDLFAAFPEMLEPLFSPSRGW
                     GVVESLIENADGSPIRRVIAEMARTEDEGLRRRLYAAAEGLFTQLAEQAVCFYDPPNV
                     MVQWTGPETFRLRIVDFEPRGRALVPGLSRIRPYVRAKVRRRCARYLKRLRETFLQTG
                     "
     gene            complement(1788..2558)
                     /locus_tag="PLZ74_10930"
     CDS             complement(1788..2558)
                     /locus_tag="PLZ74_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98873.1"
                     /translation="MERTTQNEPVNILCLKWGSRYPAYYANALYQGFRRFLHRPFRFV
                     CVTEDTQGLDQGIETFPIPENPGMSIAWPNVFLKLLVTADGFAGLQGPTLFCDLDVVL
                     TGELDCFFDFHPGKPCIIHNWIETRKLLFRPRPAIGNSSVFRFEAGKSQYICDTFLRE
                     RDRAVDPAHFRTEQAFLTYAMRECHWWPETWVRSFKRHSLRPFPLNLCSTPRLPRDTR
                     ILVFHGYPDPDEALRGYRGKRPHQHALPAPWIADYWHT"
BASE COUNT          504 a          793 c          992 g          565 t
ORIGIN      
        1 gcgttgagtt gaacttgatt gcggttgttc cggcccgcgt tttacgataa cgtaagggcg
       61 tatggggtgg cggggcaaaa cgttgggagg cagcatcggc tcgtttttcg gtccgtgggg
      121 ggcactggcc ggggctgcgg cgggtcattt tctggtcgac cgcaaaaaga cgcgccgtgc
      181 ggtcaattcg ccgctgcgcc tgctggcggt cacggctgcg gcattgcgcg aggtggccga
      241 agcggatggc cggtatacgt tgcgggaaga ccgggtgatc cgtgcggttc tcgacggaat
      301 ccgaggcgaa atcggcgcgc cgctcggccc gcacgagttg gcgtatctga ttgacgatgc
      361 cgcgcgtatc gatcaggggc ttctccggtt ggcggccttg gcgcgggaca tgcccggact
      421 cgggcatgcc gccgtggtct ggctgtggcg cgtggcggtc agcgaagcgg gcgagacgtc
      481 ggacggggcc gcccggatcg cgcgcttcgc gcaacaggcc ggcctttcgt ccggggaggc
      541 gcggttctgc gcgttgccgt acagccgcac ggcctgcgcc gacccctccg gcgttgcccg
      601 gcgcgcggac tgcgacacgc tcggtgtgcc gtatgatgcc gaccccgtgc agatcaaagc
      661 cgcgtatcgc gcgctgagtc tgacctatca tcccgataaa catgcgggtc tcgatcctgc
      721 gatccgggcg ctgaccgccg cgaaattcac tgagatcaag gcggcctacg accgactccg
      781 cggagggagc cgcgccgggt tgttggtgcg gctggcgggg gccgaccggc tggttccgta
      841 cgcggacggc gtgacggcgg cttgcttcgg gtgcggccgg agtgtccgcc tgcacgagga
      901 gtccgatccg ctgacgctac ggtgtgacgc ctgccagatg ctgttcgcaa tggagcctga
      961 acggctggcc gcgatggaga acagtcgggg cgcatccccg ttttcttgat catgacaata
     1021 tgggcacggg gttgacggct ttccgtgctc ccctcataat gcagttccgg ttacggtccg
     1081 gtgtggcggg tacggtcttc cggtgccggg ttccggatgg tgattgagga gtagatgaag
     1141 acgaggcaga tgttggacga gggccagtgg attgagatcg ggcacgggag ccgacgcaaa
     1201 tgtttccggg tcggaaacga accggtctgc gtgaaatttt accgattgcc ctcggagtac
     1261 acgcacaaga ccacttgggg ggtacggctg cacatcgggc tcgcgcggtt ccggccgcat
     1321 ttcaacgtca actgtcagga gtggcgttat caccgcgcct tacgcgaacg tctgccgccg
     1381 gacctgttcg cggcctttcc ggaaatgctc gaaccgctct tctcgccgtc gcgtggctgg
     1441 ggggtggtcg agagcctgat cgagaatgcg gacggttcgc ctatccgccg ggtgatcgcg
     1501 gagatggcgc ggacggaaga cgaagggttg cggcggcgtc tgtatgcggc ggccgagggg
     1561 ttgttcacgc agttggcgga acaggccgtc tgtttttacg acccgcccaa cgtgatggtg
     1621 cagtggaccg ggccggagac gttccgcctg cgcattgtgg actttgagcc gcgagggagg
     1681 gccttggtgc ccggcctctc gcggatccgg ccctatgtcc gcgccaaagt gcggcggcgg
     1741 tgcgcgcgat atctcaagcg gttacgggag acgtttctgc agacgggtta ggtgtgccaa
     1801 taatctgcga tccagggggc gggcagggca tgctgatggg gacgtttgcc gcgatacccg
     1861 cgcagggcct cgtccggatc cggatagccg tggaaaacca gaatccgggt gtcacgcggc
     1921 aatcgcgggg tggaacacag gttcaggggg aagggccgca ggctgtgccg cttgaagctc
     1981 ctcacccagg tttccggcca ccagtgacat tcgcgcatcg cgtaagtgag aaacgcctgt
     2041 tcggtgcgga agtgcgcggg gtcgacggcc cgatcccgtt cgcggagaaa ggtgtcgcag
     2101 atatattgcg actttcccgc ctcgaaccga aagaccgacg agttgccgat ggccggtcgt
     2161 ggccggaaaa gcagcttacg cgtttcgatc cagttgtgaa tgatgcaggg tttgcccgga
     2221 tgaaagtcga agaagcagtc cagctcgccg gtgagcacga catccaaatc gcaaaaaagc
     2281 gtggggccct gcaatccggc aaagccatcg gccgtcacca gtagtttcaa aaacacattc
     2341 ggccacgcga tgctcatgcc tggattttcg ggaatcggga aggtctcgat cccttggtcg
     2401 agaccctgcg tgtcctcagt cacgcatacg aatcggaagg ggcggtgcag aaaacgccgg
     2461 aaaccctgat acaaggcgtt tgcataatac gccggatagc gtgaccccca ttttaaacaa
     2521 aggatgttca cgggttcgtt ttgtgtggtt cgttccatga atgatactcc tccttgtatg
     2581 aaacagaata tttgagacga agccccgtgt caagagagcg aaagcaggat tacagtaccg
     2641 cctcacttgg caaagtaaat gcgactgggg cggtcgcatt tactttgaga atctcgttgt
     2701 tgccttttca ggggaatcgg ttcgtgacag gggtgcgatc gaaggtcatg cgtttcaggg
     2761 ttccattgcg gcgtcatcgc tatcgccatc gggatcggaa tcgccgccat cattacactt
     2821 gtcgctgcgg gccactgcgc gggcgcaaca gtcg
//
LOCUS       DAOSRB010000292         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_32109_length_2830_cov_5.108829, whole genome shotgun sequence.
ACCESSION   DAOSRB010000292 DAOSRB010000000
VERSION     DAOSRB010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32109_length_2830_cov_5.108829"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2687
                     /locus_tag="PLZ74_10935"
     CDS             <1..2687
                     /locus_tag="PLZ74_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98874.1"
                     /translation="LVAAAAACVACFAQGGSGSWNVQAGGRWSDAANWLDGLVAEGVG
                     ATAYFTNAPDSSVNSPTVMVDTDLLLNGLLNWVADNSTFTIAAERKSGSLDYYGIDIG
                     SSDFSIYSGRPVAFKTTLQGSGAVKLTQGLGLFIFRKNQAFTGPLYLMSGSSYKGLGY
                     QSYADSTNRVTGDLLATEEIRIDRSTLKIYGRFPRTASTGRSWELRAGEAQVTLLEED
                     TDVESFLSPGQVVTGDHIREGTYVTFLQTRSTVCLNQPLADDFSGVVTQTLSFAAAPC
                     WDVVQQVDRLVTQGSGTSGVCFQPLIDSGYGTNSVTLHVGELSGTRPVLVTVDPASGG
                     WDGRLQIDWAETFTPEITLNNTAALVLGDQAQIPTVPAPDAAFWVDASDADSLTKDGD
                     GNITRWNDTRGNGYPYATTWLDAPVYLTNALNGLPTVDFGAQGATRCLQWSEEIAGIQ
                     AVFWVIGSQEGGGSLLGRSPAGTAGNFIRGFDALRGGNDYVSRENGLIGIIEKSGENL
                     WVNGQVVTMSGAGLSGDYDMVATTLDGSRNFRASAFGRVNGQPDRYQGGQRLCEVIVY
                     TNALTTQQIQDTQAYLHKKWFGRDMPGYGAGKVDLLTTASGANARIGQAGAQPIEARY
                     VTHGGNLAVMAGSTVKVKADEALPKLTLEDGAKVVLKARKIPPAPALEGNILWLDASR
                     EEDFTFGDGAVVNTWRNRGGGSVQTVTTGSVKPVRMQDEQGRWMVDLGERDAGCCLMT
                     LSNLMVRSAFVVWLCKTNGAMPLGSLKRGYNGHNEFRENDFRRNGGAPMLNGAVRTST
                     YGAWYLDGLPIRATSTPIPTDRLMLVSAVMQGWGGRVSALGGNDFDNTPASYQYSGGM
                     QMAEVLLYDVTLTEDERRDVEAYLTWKWFGRQLSVSRT"
BASE COUNT          545 a          738 c          990 g          557 t
ORIGIN      
        1 ggctggttgc ggcggctgcg gcgtgcgtgg cgtgttttgc gcagggtggc agtggttcgt
       61 ggaacgtgca ggctggcggc cggtggtcgg acgcggcgaa ctggctggac ggccttgtgg
      121 cggaaggggt tggcgcgacg gcgtatttca ccaacgcgcc tgacagcagc gtgaacagtc
      181 cgacggtcat ggtcgacacg gatctgctgc tgaacgggct tttgaattgg gttgcggaca
      241 actcgacctt taccatcgcg gctgagcgga agtcgggcag tctggactat tacggcatcg
      301 atatcgggtc gagcgatttt tcgatctata gcggtcgtcc ggtcgcgttc aagacgaccc
      361 ttcagggcag cggggcggtc aaactgacgc aggggcttgg cctctttatc ttccgcaaga
      421 atcaagcgtt tacggggccg ctgtacctca tgtcgggctc gtcttacaag ggactgggtt
      481 accaatcgta tgcggactct acaaaccggg tgacgggcga tctgctggca acggaggaaa
      541 tcaggatcga cagatccacc ttgaaaattt acgggcggtt cccgcgcacg gcgtcaaccg
      601 gaaggtcttg ggagttgcga gccggcgagg cgcaagtgac gttgttggaa gaggacacgg
      661 acgtggagtc gtttctctcg cccggacagg tcgtcacagg cgatcacatc cgcgagggca
      721 cgtacgtgac gtttctgcag acccgttcga cggtgtgcct gaaccagccg ttggcagacg
      781 atttttcggg ggtggtcacc cagacgctct ccttcgcggc agccccgtgc tgggatgtgg
      841 tgcagcaggt ggatcggctg gtcacgcagg gcagcggcac cagcggcgtg tgtttccagc
      901 cgctgatcga ctccggctat ggaactaaca gcgtgacgct gcacgtcggc gaactttcgg
      961 ggactcggcc ggttttggta accgttgacc cggcatccgg aggctgggat ggccggttgc
     1021 agatagactg ggcggagacc ttcacaccgg aaatcacatt gaataacacg gcggccttgg
     1081 tactggggga tcaggcgcag atcccgacgg tgcccgcgcc tgatgcggcg ttctgggtgg
     1141 acgcgagcga tgcggattcg ttgaccaaag acggcgacgg gaacatcacc cggtggaatg
     1201 acacgcgcgg caacggctat ccatacgcca ccacctggct cgatgcgccg gtctatctga
     1261 ccaacgcgct gaacggcttg ccgacggtgg atttcggcgc gcaaggcgcg actcgctgcc
     1321 tgcagtggag cgaagagatc gcgggtatcc aagctgtttt ctgggtcatc ggcagtcagg
     1381 agggcggagg cagcctgctg gggcgcagcc cggcgggtac ggcgggtaat ttcatccgcg
     1441 gtttcgatgc gctgagggga ggcaacgatt atgtcagccg cgaaaacggg ctgatcggca
     1501 tcattgagaa gtcaggagag aatttgtggg tcaacgggca ggtggtcaca atgtccggtg
     1561 ccgggctctc gggcgactac gacatggtgg ccaccacact tgacggttcc agaaatttca
     1621 gggcgtcggc tttcggacgc gtgaacggcc agcccgacag gtatcaaggc gggcaacggc
     1681 tctgcgaggt gatcgtctac acgaacgcgc tgaccacaca acagattcag gacacgcaag
     1741 cctacctgca caagaaatgg ttcggtcgcg acatgccggg atatggtgcg ggcaaggtcg
     1801 atcttctgac taccgcctcc ggcgcgaatg cacggatcgg ccaagccggc gcgcagccga
     1861 tcgaagcgcg gtacgtgacg catggcggca acctcgcggt catggccggt tcaacggtga
     1921 aggtgaaggc agacgaggcg ttgcccaaac tgacgctgga agatggcgcc aaggtcgttc
     1981 ttaaggcacg caagattcct cccgcgcctg cgctggaggg caatatcctg tggctggacg
     2041 cttcccgtga ggaggatttt acctttggcg atggcgcagt ggtcaacact tggcgtaacc
     2101 gtggcggcgg cagtgtgcag accgtgacga ctggaagcgt caagcccgtg cgcatgcagg
     2161 atgagcaggg gcgttggatg gttgatttgg gggaacgcga cgcggggtgt tgcctgatga
     2221 ccctgtctaa tctgatggtg cgcagtgcgt tcgtggtgtg gctgtgcaag accaatggcg
     2281 ctatgccgtt gggtagcctg aaaagaggct ataacggcca caacgaattt cgtgagaatg
     2341 attttcggcg caacggaggt gcgccgatgc tcaatggcgc agtcagaacc tccacatacg
     2401 gcgcttggta tttggacggc ctcccgattc gggcgacctc gacacccatc ccgaccgatc
     2461 ggttgatgtt ggtttctgcc gtgatgcagg gttggggtgg acgcgtctcg gctttgggtg
     2521 ggaatgattt cgataacaca ccagcctcct atcagtattc ggggggcatg cagatggcgg
     2581 aggtgcttct ttacgatgtc acgctgacag aggacgagcg gcgcgatgtg gaggcgtatc
     2641 tgacgtggaa gtggttcggg cggcagttgt cagtatcgcg gacgtaaacg cgccttcaaa
     2701 tgggcaccgc gcctgacgaa tatccggacc gtgttctgga tgttcaaaga caagggcgca
     2761 acggagggcg gcggttggct attgggcgac acgaccagag tgagtgattt catccgggga
     2821 agttggggaa
//
LOCUS       DAOSRB010000293         2813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_32387_length_2813_cov_5.298767, whole genome shotgun sequence.
ACCESSION   DAOSRB010000293 DAOSRB010000000
VERSION     DAOSRB010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32387_length_2813_cov_5.298767"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLZ74_10940"
     CDS             <1..417
                     /locus_tag="PLZ74_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HOR98875.1"
                     /translation="RLVDMEVPPFLISSSLILAQAQRLYRKLCPICKKPCEVNPDVLA
                     ANRIDPAFFEGVTLYEASGCAKCNGIGYKGRGAVMEVLPVTDSIRAAIGRGADSATLR
                     DLAVNEGMVTLKDAGMMKVRNGITSLHAALEVTGGE"
     gene            461..1555
                     /locus_tag="PLZ74_10945"
     CDS             461..1555
                     /locus_tag="PLZ74_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOR98876.1"
                     /translation="MSEARQKSREIRVSGIGQIRKRELIPFTRQTASMLNAGMSVLAA
                     VSTLEDQCTHPGFRMVLKNLRESIESGSPVSEGLRHFPKIFDDMYVNMVAAGEKSGQF
                     APVLKRLAVMLDSSARLVRKVKSAMTYPAVIMGLAILIAGGLVTFVVPVFAEMFSGFG
                     QKLPAPTRLLVNISDFVRNRWYVLLGVALVAIVGFRKWVATPQGRYTFDKFMLRLPVF
                     GPLTQKVAVARFCRLFAQMLISGVPILDAMQIVACSMGNKVIESSILAARSGIEQGNT
                     LSASLEGKPNLPLLMVRMIAAGEKSGRIDEMLESVADTYDDEVETTLATLTSLMEPFL
                     MVFLGVVIGGIAIAMFLPIFMLGSVVSGGG"
     gene            1655..>2813
                     /locus_tag="PLZ74_10950"
     CDS             1655..>2813
                     /locus_tag="PLZ74_10950"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR98877.1"
                     /translation="MAKILIVDDEARIRMLLQSLLKANGYEASTAKDGDEALALLKKE
                     EFDLVITDLRMVPMDGMMLFREIKTLYPSMPVILLTAYASVETAIEAMKGGAFDYLTK
                     PFKVDEMLATVRRAEEHAKTHTAVVSTDPNAPLRYQFENLIASNSAMMQVCEMIQRVA
                     PTAATVLINGESGTGKEVIARTIHNTSQRHDKPWVAINCAALPEPLLESELFGHVKGA
                     FTGAHVDKEGLFETASTGTLFLDEISSLPLVLQGKLLRVLQEKEIRRVGDTKNIAVDV
                     RVIAASNTNLEQMVVQGKFRADLYYRFAVITLDIPPLRERLEDILPLARHFIRIESAG
                     SKTLPTISEEAADLLLAYKWPGNVRELENAIKHAVTFLEEDVITPELLPPRI"
BASE COUNT          563 a          894 c          811 g          545 t
ORIGIN      
        1 cgcctggtcg atatggaggt ccccccgttc ctgatctcct cctccctgat cctggcccag
       61 gcccagcgcc tctaccgtaa actctgcccg atctgcaaga aaccgtgcga ggtgaacccg
      121 gacgtgctcg ccgccaaccg catcgacccg gcctttttcg agggcgtaac gctttatgag
      181 gccagcggct gcgccaaatg caacgggatc ggctacaagg gacggggtgc cgtgatggag
      241 gttctgcctg tcaccgattc gatccgtgcc gccatcggcc gcggcgcgga ctcggccact
      301 ctgcgtgatc tggcagtcaa cgagggcatg gtcacgttga aagacgcggg catgatgaaa
      361 gtccggaatg gcatcacctc actccatgcc gcgctcgagg tgaccggcgg agaataatga
      421 cacgcccgcc ctgcgggcca tccgggaaag ggagtcgacg atgtcagaag ccagacagaa
      481 aagccgcgag atccgggtat cggggatcgg acagatccga aagagggagt tgatcccctt
      541 cacgcggcaa accgccagca tgctgaacgc cggcatgtcg gtcttggcag ccgtctcgac
      601 tctggaggac caatgtaccc accccggatt ccgcatggtt ctgaaaaatc tccgcgagag
      661 tatcgagagc ggctctccgg tctccgaagg gttgcgccac ttcccgaaaa ttttcgatga
      721 catgtacgtc aacatggtcg ccgcgggtga aaagagcgga cagttcgcac ccgtgctgaa
      781 acgactggcc gtgatgctcg actcctccgc gcgccttgtg cgcaaggtca aatcggccat
      841 gacctatccg gccgtgatca tgggtctggc catcctgatc gcgggcgggc tcgtcacctt
      901 cgtcgtgccg gttttcgccg aaatgttctc cgggttcggc cagaaactgc ccgcccccac
      961 ccggctcctc gtgaatatca gcgatttcgt ccgaaaccgg tggtatgtgc tgctcggggt
     1021 ggcactcgtt gccatcgtcg ggttccgcaa gtgggtcgcc accccgcagg gccgctacac
     1081 cttcgacaaa ttcatgctgc gtctcccggt cttcggcccg ctcacccaaa aggtcgctgt
     1141 ggcgcgtttc tgccgactgt tcgcgcagat gctcatcagc ggcgtgccga ttctggacgc
     1201 catgcagatc gtcgcctgtt cgatgggcaa caaagtcatc gaaagctcga tcctcgcggc
     1261 ccgttccggc atcgaacagg gtaatacgct tagcgcttcg ctggagggga agcccaatct
     1321 accgctgctt atggtccgca tgatcgccgc aggcgagaag agcggccgga tcgacgagat
     1381 gcttgaaagt gtggccgaca cctatgatga cgaggtcgag accaccctcg ccaccctcac
     1441 ctcgctgatg gagccgttcc tcatggtctt cctcggcgtg gtcatcggcg gcatcgcgat
     1501 tgccatgttc ctgccgatct tcatgctcgg ctcggtggtc agcggcggcg gatgatcccc
     1561 gccccgaatc ccgaaaacgt gttcttgagt tcggtacccc cttatcctat actaagttga
     1621 cttttatcgg gtacctgcgt ggacatgttt caatatggct aaaattctga ttgtcgatga
     1681 tgaggctcgg atccgtatgc tgttgcaaag cctgctgaag gccaacggct atgaagcatc
     1741 cacagccaag gatggcgatg aggctcttgc gctcctgaaa aaggaggagt ttgatctggt
     1801 catcactgac ctgcgcatgg tccccatgga cggcatgatg ctctttcgtg agatcaaaac
     1861 cctctacccc tccatgccgg tgatcctgct gaccgcctat gcgtcggtcg aaaccgcaat
     1921 cgaggcgatg aaaggcggag ccttcgatta cctgaccaag cccttcaagg ttgacgagat
     1981 gctcgccacg gtgcgccggg ccgaagagca cgccaagacg cataccgccg tggtctcgac
     2041 cgaccccaat gcgccgctgc gctaccagtt cgagaacctc atcgcctcga acagcgccat
     2101 gatgcaggtc tgcgagatga tccagcgcgt cgcgcccacc gcggccaccg tgctcatcaa
     2161 cggggaaagc ggcaccggca aagaggtcat cgcgcgcacc atccacaaca ccagccagcg
     2221 tcacgacaag ccttgggtcg ccatcaactg cgcggcgctc cctgaaccgc tgctcgaatc
     2281 ggaactcttc ggacacgtca aaggggcctt caccggtgcc catgtcgaca aagaaggact
     2341 gttcgaaacc gccagcaccg gcacactgtt tctggacgag atctcatccc tgcctctggt
     2401 gctgcagggc aaactgttgc gcgtgctgca agagaaagag atccggcgcg tgggagacac
     2461 gaagaacatc gcggtggatg tgcgggtgat cgccgccagc aacaccaatc tggagcagat
     2521 ggtggtccag ggtaaattcc gggccgacct ctattaccgt ttcgcggtca tcaccctcga
     2581 catccccccg ttgcgcgaac ggttagagga tattttgccg ctggcccgcc acttcatccg
     2641 catcgaatcc gcaggctcca agacattgcc caccatctcc gaggaagccg ccgatctgct
     2701 gctcgcctac aaatggccgg gcaacgtgcg cgaactggag aatgccatca agcacgcggt
     2761 gacgtttctg gaggaggatg tcatcacccc cgaactcctg cccccgcgca tcg
//
LOCUS       DAOSRB010000294         2667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_34799_length_2667_cov_7.826187, whole genome shotgun sequence.
ACCESSION   DAOSRB010000294 DAOSRB010000000
VERSION     DAOSRB010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34799_length_2667_cov_7.826187"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PLZ74_10955"
     CDS             complement(<1..877)
                     /locus_tag="PLZ74_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOR98878.1"
                     /translation="MKLVLGCDHGGLAIRQALLPFLQTHGINVCDLGTFDASPSDYPD
                     FAIEAAEQLVEGRADAGILICTTGVGMVIAANRFAGVRAALCESVATAVKTRTHNNAN
                     VLVLAGTMEPEVAIDIVRTFLETSFSQEERHARRIDKLERGGRLSEISHLAGADPEVH
                     AAIVGQIRQENRTINLIASENTVSRAVREASGSVLTNKYAEGYPGRRWYSGCIPVDTV
                     ETLAIERAKTLFGAEHANVQPHCGSSANMAVYFSVLDPGDTILSMSLDQGGHLTHGSP
                     VNFSGRLFNIVPYCVS"
     gene            complement(917..1807)
                     /locus_tag="PLZ74_10960"
     CDS             complement(917..1807)
                     /locus_tag="PLZ74_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOR98879.1"
                     /translation="MTTRRSFFKQAGIGTAAWAGACGLATGCASTPRIRPTPSASTDS
                     FRLGIAGYTFHKFNLIQTLEMLRKIDVHFLCIKDFHLPISSSEQEVAAFHDTCRSYGV
                     TGYGVGPIYMTTDEEVDAAFAYAKRVGTRTLVGVPYDREGKKRVASARVLRRVQERVR
                     EYDICYAIHNHGPDMPELFPTAESAMEMIADMDPRVGLCLDIGHQYRDGKDPVQALLR
                     HADRVHDIHIKNVTAADKSGKGIEMPRGRIDMPAFVRALRSVRYSGVCSLEYEKDMAD
                     PFAGIAESIGYFRGVMDATR"
     gene            complement(2235..>2667)
                     /locus_tag="PLZ74_10965"
     CDS             complement(2235..>2667)
                     /locus_tag="PLZ74_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98880.1"
                     /translation="YEFALVGVPVVPGVGKTFGEVTNADLSFNLSAMSSRQILDLRQR
                     MDTRAGGRLPVIAETPSVGLVIPRVAPDGALRSVAFINARIDVQPPITLRLRGVAPDK
                     ASATWHALRAVPVTVPLVRADGETRATLPPLAAWNCGWLEL"
BASE COUNT          558 a          799 c          796 g          514 t
ORIGIN      
        1 gcgaaacgca gtacgggacg atgttaaaga gccgacccga gaagttgacc gggctaccgt
       61 gcgtcaggtg tccgccttgg tcaaggctca tcgagaggat cgtgtcgccg ggatcgagga
      121 ccgagaagta gaccgccatg ttggccgagc tgccgcagtg cggctgcacg ttggcgtgtt
      181 cggcgccgaa cagcgttttg gctcgttcga tcgccaaggt ctcgacggtg tccacaggga
      241 tgcaaccgct gtaccaacgc cgccccggat agccttccgc gtatttgttg gtcagcacac
      301 tacccgaggc ttcacgtacc gcgcgcgaca ccgtgttctc ggaggcgatc aggttgatcg
      361 tacggttttc ctgacggatc tgcccgacaa tcgccgcatg aacttcggga tcagctcccg
      421 caaggtggga gatctcggac aggcgtccgc cgcgctcaag tttatcaatg cggcgcgcgt
      481 gacgttcttc ctgcgaaaac gacgtttcaa gaaaagtccg tacgatatca atggcgactt
      541 cgggctccat tgtgccggcc aagaccagaa cgttggcgtt attgtgcgta cgggttttga
      601 cggcggtcgc gacggactcg cagagggccg cgcgcacacc ggcaaaccgg tttgccgcga
      661 tgaccatgcc gacgccggtg gtgcaaatca ggattcccgc atcagcccga ccctcaacga
      721 gttgttccgc cgcttcgatc gcgaagtccg gataatccga gggggaagcg tcaaacgtgc
      781 ctaagtcgca cacattgatg ccgtgcgttt gcagaaaggg gagcagggct tgccggatgg
      841 ccagcccgcc gtgatcgcaa ccaagaacga gtttcattgc actccactcc gggattgaag
      901 ggtaacgggt gcgggattaa cgggtggcgt ccatcacgcc acggaagtaa ccgatcgatt
      961 ccgcgatgcc ggcaaacgga tcggccatgt ctttctcgta ttcgagacta cagacccccg
     1021 aatagcgcac cgaacgcaac gcgcgtacga acgcgggcat gtcgatccgg ccacgcggca
     1081 tctcaatgcc cttgccgctc ttatcggctg cggtcacgtt cttgatgtga atgtcatgca
     1141 cgcggtccgc gtgacgcaac agggcctgaa ccgggtcttt accgtcccgg tactgatgac
     1201 cgatatccag acagagcccg acacgcggat ccatgtcggc gatcatctcc atggcgcttt
     1261 cagcagtggg aaacagttcc ggcatgtcag ggccgtggtt atggatggcg tagcatatat
     1321 cgtactcccg cacccgctcc tgtacccggc gcagcacgcg cgctgaggct acgcgcttct
     1381 tgccctctcg gtcatagggc acacccacca gtgtccgggt gccgacgcgc ttggcatagg
     1441 cgaacgccgc gtccacctcc tcgtcggttg tcatgtagat ggggccgacc ccgtatccgg
     1501 tcaccccgta gctgcggcag gtatcatgga aagcggcgac ttcttgctcc gagctggaga
     1561 tcggcaggtg gaaatccttg atgcacagga agtgtacatc gatcttgcgc aacatctcca
     1621 acgtctggat caggttgaac ttgtgaaacg tgtatccggc aatgcccaaa cggaacgaat
     1681 ccgtcgaggc ggacggtgtc gggcggatac gcggcgtact ggcgcagcct gtcgcgagac
     1741 cacacgcgcc ggcccaagcc gcagtaccga tccctgcctg tttgaaaaac gaacgtcgag
     1801 ttgtcatcct tccctcccta caaatggaaa atcgtgtttg taaacgggac cgcccttaaa
     1861 gtcaagagca ttcgcggatt ttgaggtaat cggttcgtga caggggtgtg cgaagatcat
     1921 gcattcccga agaaaagggg gcagtgagtc gggagcgcgg gcgtccccgc ccgcaacagc
     1981 gtgtcttgcg gctgctggtt ttatccgcag atttgcgcag gttgcgcaga tttttctttt
     2041 ttgtttttga ccgcaggcgg ggagggacga gcgtctgctc gtccgcagcg tcacgccgcc
     2101 atcccgtaaa aatctctgcg tctctacgtt aaataaaccc gagtagtgcc atccctaaaa
     2161 aacctgcgta atctgtgtgt ccatctgtgg ataaaaaacc gaacagtgcc aaagcaaact
     2221 aacacgctaa tacactacag ctccagccaa ccgcagttcc atgcggcaag cggcggtaga
     2281 gtcgcccgcg tctcaccatc ggcgcgaaca agcggcaccg tcaccggtac ggcacgcaaa
     2341 gcatgccaag tggcagacgc cttgtccggc gcgacaccac ggaggcgcaa cgtaatcggc
     2401 ggctgcacat caatgcgcgc gttgatgaat gcgacgctcc ggagtgcccc atcgggcgct
     2461 acgcgcggta tgacaaggcc gacagacggc gtctcggcaa taacgggcag cctgccgcca
     2521 gcgcgcgtat ccatccgttg ccgaagatca aggatctgcc gcgacgacat cgcgctgagg
     2581 ttgaacgaca gatcggcatt ggtcacttcg ccgaacgttt ttccgacgcc cggtacgacg
     2641 gggacgccca caagcgcgaa ctcgtaa
//
LOCUS       DAOSRB010000295         2661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_34911_length_2661_cov_6.248273, whole genome shotgun sequence.
ACCESSION   DAOSRB010000295 DAOSRB010000000
VERSION     DAOSRB010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34911_length_2661_cov_6.248273"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2455)
                     /locus_tag="PLZ74_10970"
     CDS             complement(<1..2455)
                     /locus_tag="PLZ74_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98881.1"
                     /translation="MPRIYHFVICLAMLMVPTVGAWAQIRRLEPAGVARTELGVPQFQ
                     IRGADALGLTAVPSDLHVLPDGRVLVVAGRTIAWGDGVRWESFRQSTEERPSQSRHVA
                     VAPDGTIYIGVRGGISRIEFGEDRCWRTRMVVAVSNLDDSGPADDQREVVEVGDEWLW
                     HGRSGPFRLWHPGSSVRMVGQLDQIAHAFFFRGAYYASSMTTGSLWRLGSDAMHPVLQ
                     APDVWINDTVISSTPFDSGRLLVGTFGRGLRLFDGERFQPFASDSVLAAPARISALCS
                     AGDGFFAAAVDTLGIVFFDRQGRVIQIADRSVDHRLGGVRQLVAGADGTVWGLLKEGI
                     VRVRFPSHISDFVPLFGTAINTVNVCRYGSELWFMGDGRIFRGVYDEGGRLTRLRVDS
                     PPDAYVTALSYAGGFQIAGTDKGSFYWAESGWVPFAPSTNNLRVAVPEAFDGRWLYLA
                     DREIGWLHLRSGRLEREAFPVPELSRTFHAVRDENGAVWTEMGSGRIGRVQIVDSHPQ
                     LTVFTAADGLFPSWCQLFEIDGVARVNVSGRTLRFDKASQRFLPDDEFLRRFPQCREI
                     NCRPGRDALGRIWVVADNVLRIYRETPEGLVPDPAERSFRGHMPFYFTFEDNGVVWTQ
                     EDCGLFRYDPAALELEAGTLSARITQVDLAGSGRTFFTSQGALPDLSFDDRSLVAHFI
                     AVDAPMDAAVTFEVQLEGVDPGWNNVGSAGSAAYRNLHEGAYRLLVRPCIGDLPGKTD
                     TLVFFIMPPWYRAWWAYLSYVLGCAALVGGFLAMQRRKRRQLESLVGERTAALNESNR
                     QLERQLATIRTLSQAIEQSP"
BASE COUNT          607 a          890 c          748 g          416 t
ORIGIN      
        1 tggggctttg ttcgatggcc tgggagagcg tgcggatcgt ggcgagctgg cgctcgagct
       61 gccggttgga ctcattgagc gcagcggtac gctctcccac gagactctcc aactggcggc
      121 gtttacgacg ctgcatcgcg aggaagccac cgacgagcgc ggcacagccc agcacatagg
      181 ataggtaggc ccaccaggcg cggtaccacg gcggcatgat gaaaaagacc agcgtatccg
      241 ttttcccagg caagtcgccg atgcaggggc ggacaagcag ccggtacgcg ccctcgtgga
      301 ggttccgata agccgcggag ccggcactcc cgacattgtt ccagcctggg tcgacgcctt
      361 ccaactgcac ctcgaaggtg accgctgcat ccatcggcgc atcgaccgcg atgaaatgcg
      421 ccaccagtga ccgatcatcg aaagaaagat cggggagagc tccctgactg gtgaaaaagg
      481 tccgtccgct gccggccaga tcaacctggg tgatccgcgc gctcagtgta cccgcctcca
      541 actccagcgc cgcaggatcg tagcggaaaa ggccgcaatc ctcctgtgtc caaaccactc
      601 cgttatcctc aaaggtgaaa tagaaaggca tatgcccacg gaaggaccgc tcagccggat
      661 cgggcaccag cccctctggg gtttcgcggt agatgcgcag gacattgtcg gccacgaccc
      721 aaatccgccc cagggcatcg cgcccagggc ggcaattgat ctcgcgacat tgtggaaagc
      781 gccggaggaa ttcgtcgtct gggaggaacc gctgggatgc tttgtcgaac cgcaacgtgc
      841 gccccgaaac gtttaccctc gcgacaccat cgatctcgaa cagctggcac caggagggga
      901 ataatccgtc ggcggcggtg aagaccgtga gttgcgggtg gctatcgacg atttgcacgc
      961 gaccgatgcg accgctgccc atctctgtcc agacagctcc attttcgtcc cgcacggcat
     1021 gaaatgtacg cgacaactcg ggcacgggga acgcctcgcg ctcgagccgg ccggaccgta
     1081 aatggagcca gccgatctcg cggtccgcca gataaagcca gcgcccatca aaggcctccg
     1141 gcacggcgac gcgcagattg tttgtgctcg gagcaaacgg cacccagcca gattccgccc
     1201 agtaaaagga tcctttgtcc gtcccggcga tctggaaacc gcccgcgtac gaaagcgccg
     1261 tcacgtaagc atcgggtggg gaatccaccc gcagacgcgt caaccgtcca ccctcgtcat
     1321 atactccgcg gaaaatgcgc ccatcaccca tgaaccaaag ttcggaacca tagcgacaga
     1381 cgttgaccgt gttgatggcc gtcccaaaga gcggtacgaa atcagaaata tgcgacggga
     1441 aacgcacccg gacgataccc tccttgagca gtccccagac cgtgccatcc gcccccgcca
     1501 ccagttgccg cacgccaccc aaccgatggt ccaccgatct atccgcgatc tggatcacgc
     1561 gcccctgccg gtcaaaaaaa acgatcccca acgtatcgac cgccgcagcg aagaaaccat
     1621 ctcccgcaga gcacagggcg ctgatgcgag ccggtgccgc caacacagaa tcggaagcaa
     1681 acggctggaa acgctctccg tcgaacaacc gtaacccccg cccaaacgta cccacgagca
     1741 gacgccctga atcaaacggc gtcgacgaga tcacggtgtc atttatccag acatctgggg
     1801 cctgcaaaac cgggtgcatg gcatcggagc ccagccgcca aaggctgccc gttgtcatcg
     1861 agctcgcgta atacgctcca cggaagaaaa acgcatgcgc gatctggtcg agttgcccga
     1921 ccatgcggac tgatgagccc ggatgccaaa gccgaaacgg accgctgcgc ccatgccaca
     1981 gccactcatc accgacctcg actacctcac gctggtcatc ggccgggccg gaatcatcca
     2041 gattggacac cgcgacaacc atacgcgtgc gccaacaacg gtcttcccca aattcgatcc
     2101 gggatatccc gcccctgaca ccgatgtaaa tcgtcccatc gggcgcgacc gccacatgcc
     2161 tggactgcga aggcctctcc tcggtggact gccgaaacga ctcccaccgg acgccatcgc
     2221 cccaggcaat cgtccgaccg gccaccacaa gcacgcgacc atccggcagc acatgcagat
     2281 ccgacggcac tgcggtcaac cccaacgcat ccgccccgcg tatctggaac tgcggcacac
     2341 caagctccgt acgcgccacg cctgccggct ccaacctgcg gatctgagcc catgcaccca
     2401 ccgtcggcac catcagcatg gcgaggcaga tgacaaagtg gtatatccgg ggcatgggat
     2461 taggaacgct ccacacagag gacgcgcggg gcaagtggca gcgtgccgga gtaggatgcg
     2521 atttttccaa ggaggcaaga tcgggtaatt tggctacgcc atcctgacgg cttgctttca
     2581 aataaaacat tggttgcggt ttcccgttta tgaaacgcgc cctcatcacc ggcatcacag
     2641 gacaggatgg ttcatatctg g
//
LOCUS       DAOSRB010000296         2657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_34984_length_2657_cov_7.607610, whole genome shotgun sequence.
ACCESSION   DAOSRB010000296 DAOSRB010000000
VERSION     DAOSRB010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34984_length_2657_cov_7.607610"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLZ74_10975"
     CDS             <1..255
                     /locus_tag="PLZ74_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98882.1"
                     /translation="DTDPDGDLKNVNVGHTPYPYPMTPPPCHEPITEYTNVFAPYWGT
                     IPAGGVSRESVMCLHVGFSGGFVDEPRFSVLSWIHLGKQL"
     gene            255..1082
                     /locus_tag="PLZ74_10980"
     CDS             255..1082
                     /locus_tag="PLZ74_10980"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR98883.1"
                     /translation="MRDNPDTRLDRTLCSLPLRRELSSVLHFLHTQARGARVGLDIGF
                     THAGVSQILRQTGGYWMSVETSPERRNLVASVLGSDTVLCAGADGTLPFEDKQFDVVV
                     MAHGTLLSGAAAADAIRECHRVLKTGGHFILTVEYRKRVSIAAAINRQRVVTGAGVRY
                     NAEDIFHLLKDGFDVLGFRHTCRFWVQLVRQWVDRRQASGMCGGMQNRIRFLYGLARI
                     LDIPLFLSRGYQMTVYGRRKGWRGKHSRVISGITPVSDALLSDPRRERRGMSLMRYK"
     gene            1079..2599
                     /locus_tag="PLZ74_10985"
     CDS             1079..2599
                     /locus_tag="PLZ74_10985"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR98884.1"
                     /translation="MNRAACLAAAWFLVLSCSGAGPEESASDTGADPVREAVSLFINA
                     RTAGGLDAFIVAVERVRKLAESGQPFFRFLLAVYAAHEPLCKLTDTARQRYLEEGRPL
                     MSRHAETGNALAQYLVGVDCELNLAQPEDARRWYERAAMQGQALAQNRLGLMYYQGQG
                     VAPDDGRAAAYFRQAALQADMNGEYNLATLYAAGRGVPKDEELAFHLFRRAAEKGHAH
                     AMNNLGWLYQKGVGVAADPARAAEWYRKAAESGNADGQFNYGFLCAQGVGVPQDDREA
                     GLWYWRAAEQGHVEAMVSIGMLYRDGRGLKGDDFKALNWFAKAAEHRHAGAQFFIGQM
                     LEQGRGLPRDTERAFRWYQKSAQQGYPRAHHALGTLYYRGRGVEKDYVKAAGHFALAA
                     EKGLASSQNNLGMLYLRGEGVERDEKQAAAWFAKAVRQGVAGAAFNLAQLYEYGRGVD
                     ASHTKAVQLCKEAALRGHVGAADWLLQNGYTAWAKPHREKKNRDAQVSEGLEDQPF"
BASE COUNT          476 a          724 c          958 g          499 t
ORIGIN      
        1 gataccgatc ccgatggcga tttgaagaac gtgaacgttg gccatacccc gtacccgtac
       61 ccgatgactc cccccccctg tcacgaaccg attactgaat atacgaatgt attcgctccg
      121 tactggggaa cgatccctgc cggcggggtt tcaagggaat cggtgatgtg cctgcacgtc
      181 ggtttttcag gcgggtttgt tgacgaaccc cgcttcagcg tgttaagctg gattcacctt
      241 ggaaagcagt tgtgatgcgc gataatccag acacacgctt ggatcggact ctgtgttccc
      301 tcccgttgcg gcgtgaactc agttctgtcc ttcatttcct gcacacgcag gcgcggggcg
      361 cccgggtggg cttggacatc ggtttcaccc atgccggtgt gagccagatt ctgcgtcaga
      421 cgggcggtta ctggatgagt gtggaaacct cgcccgagcg ccggaacttg gtggcatcgg
      481 ttctgggcag cgataccgtg ttgtgcgcgg gcgcggacgg gacgttgccg ttcgaggaca
      541 agcagtttga tgtggtggtg atggcgcacg gaaccctgct gtccggcgcg gccgctgccg
      601 atgctatacg cgagtgccac cgggtgctca agaccggggg gcatttcatt ctgacggtcg
      661 agtaccgcaa gcgggtcagc atcgctgcgg cgatcaaccg gcagcgggtg gtgacggggg
      721 ccggtgtccg gtacaacgcg gaggatattt ttcatctgct aaaagacggc ttcgacgttc
      781 tggggttccg ccacacctgc cggttctggg tccagctggt gcggcaatgg gtggaccgcc
      841 gccaagcctc cgggatgtgc ggcgggatgc aaaaccgaat ccgtttccta tatgggttgg
      901 cccggattct cgatatcccg ctctttctgt cgcgagggta tcagatgacc gtgtacgggc
      961 ggcggaaagg gtggcgtgga aaacactccc gggtgatttc gggaatcacg ccggtttcgg
     1021 acgcgctgct ctccgatccc cgacgcgaaa ggcgcggcat gtcgctgatg aggtacaagt
     1081 gaatcgggcg gcgtgtttgg cggcggcgtg gtttctggtg ctgtcgtgca gcggcgccgg
     1141 gccggaagaa tcagcgtccg acaccggggc cgatccggtc cgggaagcgg tctccctgtt
     1201 tatcaacgca cggaccgcgg gggggctgga tgcgttcatc gtggcggtcg agcgggtgcg
     1261 gaagctggcg gaatcgggcc agccgttttt tcgtttcctg ctggcggtct atgcggcgca
     1321 cgagccgctc tgcaagctaa ccgacacggc gcggcaacgc tatctggagg agggacgccc
     1381 cctgatgtcg cggcatgccg agaccggcaa cgcgctggcc cagtatctgg tcggtgtgga
     1441 ctgcgaactc aatttggcgc agccggagga cgcgcgccgc tggtatgagc gcgccgctat
     1501 gcagggtcag gctctggccc agaatcggct gggcctgatg tactatcaag ggcagggggt
     1561 tgcgccggat gacggccggg ccgccgcgta ttttcgtcag gccgcgctgc aggctgacat
     1621 gaacggcgag tacaatctcg ccacgctgta tgcggcaggg cgcggagttc cgaaagacga
     1681 ggaactggcg tttcatctgt tccggcgggc agcggaaaag ggccatgccc atgccatgaa
     1741 caatctgggg tggctgtatc agaagggggt gggcgttgcc gcagacccgg cgcgggctgc
     1801 ggagtggtac cgcaaggcgg cggaaagcgg gaatgcggac gggcagttca attacggttt
     1861 cctgtgcgcg cagggtgtcg gggtgccgca ggatgaccgt gaggcggggc tctggtattg
     1921 gcgcgcggcg gagcaggggc atgtcgaggc catggtgagc atcggcatgc tttaccgcga
     1981 tggccgggga ttgaaaggcg atgacttcaa ggcgctgaac tggttcgcca aagcggcgga
     2041 gcaccggcat gcgggggcgc agttctttat cgggcagatg ctggaacagg ggcgcggtct
     2101 gccccgtgac accgagcgcg ctttccgctg gtatcagaag tccgcgcaac agggttaccc
     2161 gcgcgcccat catgcgctgg ggacgctgta ctaccgcggc cggggggtgg agaaggatta
     2221 tgtgaaggcg gccggacatt ttgccctggc cgcagaaaaa ggtctcgcct cttcccaaaa
     2281 caatctgggg atgctctatc tgcgcggcga gggtgtggag cgcgatgaaa aacaggcggc
     2341 ggcatggttc gccaaggccg tccggcaggg agtcgccggg gcggctttca atctggcgca
     2401 actctacgag tatggccgcg gcgtggacgc gagccacacc aaagcggttc aactctgcaa
     2461 agaagccgcg ttgcgcggac atgtgggcgc tgccgattgg ctgttgcaaa acggctacac
     2521 cgcctgggcg aaaccgcacc gggagaagaa gaaccgggac gcgcaggtgt cggaagggct
     2581 ggaggatcag ccgttctgaa acaagggcgc gcggcggatt gacagcggcg gaagaaaagc
     2641 gttatggtga aacgagg
//
LOCUS       DAOSRB010000297         2612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_35804_length_2612_cov_6.403989, whole genome shotgun sequence.
ACCESSION   DAOSRB010000297 DAOSRB010000000
VERSION     DAOSRB010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35804_length_2612_cov_6.403989"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLZ74_10990"
     CDS             complement(<1..184)
                     /locus_tag="PLZ74_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOR98885.1"
                     /translation="MAKVLGIDLGTTNSCMSVMEGGEPVVVPNAEGMRTTPSIVAFTK
                     SGERLVGQAAKRQAVTN"
     assembly_gap    937..1036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1037..2400
                     /locus_tag="PLZ74_10995"
     CDS             <1037..2400
                     /locus_tag="PLZ74_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98886.1"
                     /translation="STPPQRPAPSANRDPGSRIFSGTRADRTEPRKPSRPAPSVSTPP
                     QRPAPSANRDPGSRIFSGTRTAPSQRSDQRSESPSPASVRSVPQTPSVSRPDSRTRSD
                     TPRRITLDKRNGIRRGNDRPLRGESRTHLPERFASIQRNDRASPLSLRAPDGLSDSRT
                     PRLTPRRESVRGRPESRLRNVRPTPFRAPPRVYRPSPHRHPTARYRHRSPYVFDPWTF
                     RHHHRGFSHIVYRPVPIYAASGMSFFWSSGAFALGFSTYSWPSYGYTYTRYYDSWHDG
                     YWGYSNLYLGGWRNGWYGGFSYIYNPWPVYRTYYFYEPAPVVTRVETVYVEQAAPATV
                     TATTTSSATVAPPATSAVEEPVGAWAEAPAPERVEAQSYRCFCACGCNGTRPCFCDYP
                     CGAEYEDITTRTDLRLGYVSYAESLNPEAIWASYAGLDYATDEVYDQPDTETAAYSDR
                     DLL"
BASE COUNT          473 a          845 c          664 g          530 t
ORIGIN      
        1 ggttggtcac cgcctgccgc ttggccgcct ggcccaccag acgctcgcca ctcttggtga
       61 acgcaacgat ggacggcgtc gtgcgcatcc cttcggcgtt cggaaccacg accggctcgc
      121 cgccttccat tacggacatg caggagttgg tcgtccctaa gtcaattccc aaaacttttg
      181 ccattttacg ctccgtttca tccgctctaa ctgaacaccc tatacgcgga tacggggtat
      241 aatgcacctt gtgtgccaaa atcctttcga catccggact gttttattta taacctgttt
      301 ttttgcaagt ggttagaaaa aatatccttc cttaatttat ttggcctgaa gggtgcggtt
      361 tcttgttttg gcacacttgt catggcacag ttttcacaaa gtgggtcata attgctcgcc
      421 gcgtcacggc ttctttcttg ccgttttaat cggatgttta aaatatggcc gatcgaaggc
      481 aatacgaaga ggcataaggc gtttatcttt acgtgtgggg aggtgcaagt ggtcttgacg
      541 ggcccgcccc gagcccctaa actgcggatt atgagccttt gaccgaaaat ggctggatgg
      601 gaagaaagga gcgcaccatg aaaacaacaa gatggctcat tgttttgatg gccgtaacgg
      661 ggttcgggtt ggcgtcatcg gctcgggagc gctcctcgtc tagcagccga ccgcgtggcg
      721 cgcctgcctc ttccaaacaa agcgcatcct cctatcaaac aggacgtaac gcttcatcat
      781 cctcacgtcc ctcagcaccg cgctcccaaa tggcggctca accgcggtca gcgcatgtgc
      841 cgtcgccgaa aaccccgtac cgcatgccgg caccccgcgt ctcacaaccg ttctcctcat
      901 ccgtctcccg gcagcagtcc cggcccgcgc cctccgnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnntctc cacacccccg caacggcccg cgccctccgc caaccgcgat
     1081 cccggttcgc gcatcttcag cggcacccgc gccgatcgga cggaaccccg aaaaccgtcc
     1141 cggcccgcgc cctccgtctc cacacccccg caacggcccg cgccctccgc caaccgcgat
     1201 cccggttcgc gtatcttcag cggcacccgc acggccccct cacagcgttc ggatcaacgg
     1261 tccgaatccc catccccggc atccgtccgt tcggtgccgc aaactccaag cgtttcccgg
     1321 ccggattccc ggacacgctc ggacacgccc cgccggatca cgctggataa aaggaacgga
     1381 atccgccgcg ggaatgaccg tccgttgcgc ggcgaatcgc gcacgcacct tccggaacgc
     1441 tttgcttcca tccagcgtaa tgatcgcgcc tccccgctca gccttcgcgc gcccgacgga
     1501 ctctccgatt cccgcactcc ccgcttgacg ccccggagag aaagcgtacg gggccgtccg
     1561 gaaagccgac tccgcaatgt gcggcccaca ccgttccgcg cgccaccccg tgtgtaccgt
     1621 ccttccccgc accggcatcc gacggcgcga taccgccacc gctcgccgta tgtgtttgat
     1681 ccgtggacgt tccggcatca ccatcgcggg ttctcgcaca tcgtctaccg gccggtgccg
     1741 atttacgcgg ccagcggaat gagtttcttc tggtcaagcg gcgcgttcgc actcggcttc
     1801 agcacctact cgtggccgag ttacggttac acgtacacgc ggtattacga cagttggcac
     1861 gacggatatt ggggatactc caacctctat ctgggcggat ggcgcaacgg ttggtacggc
     1921 gggttctcct atatctacaa tccgtggccg gtttaccgga cctactactt ctatgaaccc
     1981 gccccagtgg tgacgcgggt ggagaccgtg tatgtcgaac aggcggcccc ggccaccgtg
     2041 accgcaacca cgacctcatc cgcaacggtc gcccctccgg ccacctcggc ggtggaagag
     2101 ccggtcggcg catgggcgga agcccccgcg ccagaacggg tcgaggcgca atcgtaccgc
     2161 tgtttctgcg cttgcggctg caatgggaca aggccctgct tctgtgacta tccgtgcggt
     2221 gcggaatatg aggacatcac gacccggacc gatctccgcc tcggatatgt ctcgtatgcg
     2281 gaatccttga atccggaagc gatctgggct tcgtatgcgg gattggatta cgccaccgat
     2341 gaggtgtatg accaaccgga cacggaaacc gcagcctact cggaccgcga cctgctctga
     2401 catgcaaccc gatggtcttt ccgttccatc ttttccgtac gcagctcgcc cgcatccgga
     2461 atcagaatta gcctgccgcg cggcttcgct tcggcgggaa gaaatccgtg attaacatga
     2521 tgtcgttcaa tcctgaaaat cctgtcatcc tgtcaattta taggcaaggt tttgtgttcc
     2581 agtgttgaaa tcatgaatct gttcaaacgc tt
//
LOCUS       DAOSRB010000298         2605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_35934_length_2605_cov_3.332941, whole genome shotgun sequence.
ACCESSION   DAOSRB010000298 DAOSRB010000000
VERSION     DAOSRB010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35934_length_2605_cov_3.332941"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..895
                     /locus_tag="PLZ74_11000"
     CDS             248..895
                     /locus_tag="PLZ74_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOR98887.1"
                     /translation="MPRTVSYSTGEEIANSITHGLGAAFGVAGLAVLVTLAAIQGDPL
                     RVVSFSVYGASLVTLYLFSTCYHGVRAPRLKRFFRVMDHSAIFLFIAGTYTPFTLVSL
                     QGGVGWWLFGVIWGLAATGILLKLFFTGRFGILSTIVYVAMGWCVLVAIKPVVAAIPF
                     GCFLWLLVGGLSYTLGLIFYAAQRLPYNHAVWHVFVMAGSAAHFVAIFLYVLPNR"
     gene            1055..>2605
                     /locus_tag="PLZ74_11005"
     CDS             1055..>2605
                     /locus_tag="PLZ74_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98888.1"
                     /translation="MTMRYGLGEGSVLCKKQSYGGSKTRGRVRLLALLLVGAVFVGGA
                     AFPAYAAFSSTSCVIAGGAAWGIDRVQPVAVSATANKNIIAYQVTDGNGNWSGWNYIS
                     AGGSVSYTLYPQLYQVNGAVYGFQIQFLDAGYTVGSAYDTIYLDLDNPTISTPTDEGV
                     YKAGSTVRFFWTASDGTSGIKDYYMIVGTQPGWNDKFAGWIGAQPGYYDISGCSDGVT
                     YYCQMYAQDNAGRTSAWSGVSNGIKVDLHPPTVQAPYDNGLWDDGTVDFSFLGFDSSS
                     GIAGYVYEVAANTSFNPVLATGTVSGNFYTYPDGISGVTYYCRVKAVDNVGRMSDWSS
                     ASDGVMVDLGPPTMTTPQPIAPYSTSDNITFSWTATDDNLVITDYYMQVGTAPGLADK
                     LDGWIGPQPGYYNIPGCADGKTYYCRMMAKDKAGRVSEPTAWSSGITVDLTNPAPPGT
                     PTAGNVWAPATVTFAWVPSWDLSGVAYYVVEIRNWPDWPAIGVSGTTTTNTYSFTGVN
                     GNSYFCKVR"
BASE COUNT          486 a          747 c          754 g          618 t
ORIGIN      
        1 gggtcttctc tcgcgcggcg ccccttcagg aacggctatt tccgcctccg gcaatgcgtc
       61 tgtggacatc cggggctaga gcgatacgta tatcattttt ttgcgttcaa agcgagcttt
      121 ttctcttcga ctgtgatatg ctatccttta tgatgtccac cgtgaaaggc cgtccccgcg
      181 ccgcatgcac gcggcgcgcc cgcccccaag gaacccttgc gcaaccatga tcgcgccgtc
      241 gccgcacatg cctaggactg tttcgtattc tacaggcgaa gagattgcca atagcatcac
      301 ccatggactg ggcgcggcgt tcggcgtcgc cggcctggcg gtgttggtga cgcttgccgc
      361 tattcagggc gacccgcttc gggtggtgag ctttagcgta tatggggcct ctctcgtaac
      421 gctgtatctc ttttcaacgt gctaccatgg agtccgcgcc ccacggctaa aacgcttttt
      481 ccgcgtcatg gaccattcgg ccatattttt gttcatcgcc ggaacctata cccccttcac
      541 cctggtcagt ttgcaaggcg gagtgggatg gtggcttttt ggagtgattt ggggcttggc
      601 ggcgacgggc atcttgctga agctattttt cacgggccga ttcggcattt tgtcaactat
      661 cgtttacgtg gcgatggggt ggtgtgttct tgtggcgatc aaaccggtgg ttgccgcaat
      721 tcctttcggt tgctttcttt ggctgttggt ggggggattg tcctacacct tgggactaat
      781 tttctacgcc gcccaacgtt tgccctacaa ccacgccgtg tggcatgtct tcgtaatggc
      841 cggaagcgct gcccattttg tcgccatttt cctctatgtc ctccccaatc ggtagatttt
      901 cgcaattttc tcaccgattc tacgaaactc actacaaaag tgttcttcgt agcgttttcc
      961 ttgacagaat cccgttctta cactatagtt agaacaggtg attttgtctt ttggcgcagg
     1021 tgaggggttt gtcataccta gcaaggagca tcacatgacc atgcggtacg gcttagggga
     1081 gggctctgtc ttgtgtaaga aacagtctta cggcggttcc aagacaagag gacgcgtccg
     1141 attgttggca ctgttgcttg taggcgccgt attcgtggga ggggccgcgt ttccggcata
     1201 tgccgcattt tcttccacat catgcgtcat cgcgggcgga gctgcgtggg gcatcgaccg
     1261 ggtgcagcct gtcgccgtat cggccaccgc caataaaaat atcattgcgt accaagtcac
     1321 cgacggcaac gggaattggt cgggctggaa ctatatttcc gcgggcggaa gcgtttcgta
     1381 cactctctac ccacaactgt atcaggtgaa tggcgccgtc tatggtttcc agattcaatt
     1441 tctggatgcc ggatacacgg tcgggagcgc ctacgacacc atctatcttg atctcgacaa
     1501 tcccaccatc tccactccca ccgacgaggg cgtttacaag gcgggcagca cggttcgctt
     1561 tttttggacg gccagcgacg gcacgagcgg catcaaggac tattacatga tcgtgggcac
     1621 ccaacccggc tggaatgaca agttcgccgg ctggattggc gcgcaacctg gatactatga
     1681 catttcggga tgctcggacg gagtgaccta ctattgccag atgtatgccc aggacaatgc
     1741 tggccgcacg agcgcatggt ccggggtctc caacggcatc aaggtggacc ttcatccgcc
     1801 aaccgtgcag gctccgtacg acaatgggct gtgggatgac ggcacggtgg atttctcttt
     1861 cctgggcttt gattcctcct cgggcatcgc ggggtatgtc tacgaagtgg ccgccaacac
     1921 gagtttcaac ccggtgttgg cgacgggcac cgtctccggc aatttctaca cctatccgga
     1981 cgggatatcg ggcgttacgt actactgccg cgtcaaggca gtggacaacg tgggccgcat
     2041 gagcgactgg tcatccgcga gcgacggcgt catggttgat ctcggccccc ccaccatgac
     2101 aacgccgcag cccatcgcgc cgtattcgac gagcgacaac atcaccttct cgtggaccgc
     2161 caccgacgac aacctcgtca tcaccgatta ttatatgcag gtgggcactg ccccggggct
     2221 ggccgataag ctggacggct ggattggacc gcaacccggt tactacaaca ttccgggatg
     2281 cgcggatggc aagacctatt actgccgcat gatggccaag gacaaggcgg gccgcgtgag
     2341 cgagcccact gcatggagca gcggcatcac ggtggacctg acgaatccgg cgcctccggg
     2401 cacgccgacg gcgggcaatg tgtgggcgcc ggcgacagtg accttcgcgt gggttccgtc
     2461 gtgggacctt tccggcgtcg cctactacgt ggtcgaaatc cgcaattggc cggattggcc
     2521 tgcgatcggg gtcagcggca cgacgacgac gaacacgtac agtttcactg gcgtcaacgg
     2581 gaacagctat ttctgcaagg tccgt
//
LOCUS       DAOSRB010000299         2604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_35944_length_2604_cov_4.905845, whole genome shotgun sequence.
ACCESSION   DAOSRB010000299 DAOSRB010000000
VERSION     DAOSRB010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35944_length_2604_cov_4.905845"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLZ74_11010"
     CDS             complement(<1..256)
                     /locus_tag="PLZ74_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98889.1"
                     /translation="MSTSRGQRAGHLAPTAGAAPQRPAAEAAFVPGELGQVTAEAVTI
                     GLPTLRAKTNVEMRGVGRKFSGLYHVETVRHRIDGSGYSCE"
     gene            complement(253..1140)
                     /locus_tag="PLZ74_11015"
     CDS             complement(253..1140)
                     /locus_tag="PLZ74_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98890.1"
                     /translation="MTAHNILPAPLVLVEINGQRITRDLTDRLVAFVYEDHEAALDLL
                     EITLADPYLQCLDDPLFQEGHDIRVRFGYADNLSPTKVGVIKEISYDFPETGVPTITL
                     KAYDKGCTLASEQVQRVWQRPGGIRASDIATAIATEHGLTPVVTATVDRVPRLHQSKQ
                     SDAQWLAQLAKTARTVDGKGTTGFVFYVEGEELHFHPRGLEQPPVMALEYFTDRQGVL
                     RSFRPATRTQGDAASGQSTTAVGVNPATKTAATTTAENASTPERPVLGSKTVQGAGKA
                     SGRVVIDANTGTRVTGGRS"
     gene            1264..1509
                     /locus_tag="PLZ74_11020"
     CDS             1264..1509
                     /locus_tag="PLZ74_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98891.1"
                     /translation="MSRVIVHEQVDVNANFGPDGITPRAFRLHGHTVRVTQVHQRWQG
                     RHGATRLHYFTVTAAERPYTLCFNADDGCWMLKEHTP"
     gene            1632..1778
                     /locus_tag="PLZ74_11025"
     CDS             1632..1778
                     /locus_tag="PLZ74_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006942260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98892.1"
                     /translation="MSKDKQTSPRAASAASKVLRDGRTGAASKTAAGSALAQTPAKPS
                     KKRS"
     gene            1825..2202
                     /locus_tag="PLZ74_11030"
     CDS             1825..2202
                     /locus_tag="PLZ74_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR98893.1"
                     /translation="MTQNSVAVGKRIKEVREARKATDPRFSLRRFADAVGLSPTFISR
                     LEHGTGVLPSPANLERIAEVLEIEAKELFELAQRVEPQVKDLLLEQPAWADFLRTARS
                     RGWDADAVKQFVEDHYTEPDTDA"
     gene            2199..>2604
                     /locus_tag="PLZ74_11035"
     CDS             2199..>2604
                     /locus_tag="PLZ74_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOR98894.1"
                     /translation="MKRTRFLPEQDIERAALVLAERYCHRFSQPLTPHLPLEELIECV
                     LELSFRVDDLTALFGTADILGATKVDERLILVDMSLDPCEHPEKEGRYRFTLAHEIGH
                     WELHRQSVPTAAQRTLFDTDPLGTILCRASQYD"
BASE COUNT          487 a          826 c          795 g          496 t
ORIGIN      
        1 gttcacaact gtagccgctc ccatcgatgc ggtgccggac ggtctccacg tgatagaggc
       61 cactgaactt ccgtccgact ccgcgcatct ccacattcgt tttggcccgc agcgtgggca
      121 ggccgatggt caccgcctca gcggtaacct ggcccaattc tcccggcacg aaggccgctt
      181 cggcggcggg ccgctgcggg gccgcccccg cggtcggcgc cagatggccg gcgcgctgtc
      241 cgcggctggt gctcatgacc ggcctcccgt cacccgcgtg cccgtattag catcgatgac
      301 cacgcgtccc gaagccttgc cggcgccctg cacggtcttg ctgcccagca ccgggcgctc
      361 cggggtggat gcattctccg ccgtagttgt cgccgccgtt tttgtcgccg gattgacgcc
      421 gaccgcggtg gtcgactgcc cactggccgc gtcgccctgg gtacgcgtcg cgggacggaa
      481 cgaccggagg accccctggc gatctgtaaa gtattccaac gccatcaccg ggggctgctc
      541 caacccacgc gggtggaagt gcagctcctc cccctcgacg tagaacacga aaccggtggt
      601 gcctttcccg tccaccgtgc gggcggtttt ggccagctgc gccaaccatt gggcgtccga
      661 ctgcttgctt tgatgcagcc gcggaacgcg atccaccgtc gccgtcacca ccggcgtcaa
      721 cccgtgctcg gtagcgatgg ccgtcgcgat atcgctcgcg cgaatcccac ccggacgctg
      781 ccagacgcgt tgtacctgct cggacgccag cgtgcacccc ttgtcatagg ctttcagcgt
      841 gatcgtcggc accccggttt cggggaagtc gtaactgatc tccttgatga cgcccacttt
      901 ggttggcgac aggttgtcgg catagccgaa gcgcacgcgg atgtcatggc cttcctggaa
      961 gagcgggtca tcgagacatt gcagataggg atcggccagc gtgatttcca gcaagtcgag
     1021 cgccgcttcg tgatcctcgt agacgaacgc caccaggcga tccgtcagat cgcgcgtgat
     1081 ccgttggcca ttaatctcga ccagcacgag cggtgctggc aggatgttgt gcgccgtcat
     1141 agaccccctc aaaaaaagcc ggcaatattt tgcagaacat gcaaacaaaa gtgttgtgtt
     1201 atcgaccacg tccttgctat acttgccatg cagtgcgctg cgtatgaaag ggcacccctc
     1261 gcgatgagcc gtgtcattgt gcatgaacag gttgacgtca acgccaattt tgggccggat
     1321 ggcatcaccc cccgtgcctt tcgtctgcac ggacataccg tgcgagtgac ccaggtgcat
     1381 cagcgctggc aaggccggca tggcgcgaca cggctccact atttcacggt gacggcggcg
     1441 gagcgcccgt atacgttatg cttcaatgcc gacgatggct gctggatgct caaggaacac
     1501 accccctaac cggcgtgcgc ctctatatgt ctatattggc agccgtcgta aatatggccc
     1561 acgccccctt gacagaacgg cactttttgt ttactattta gggcacagta aagtttgaaa
     1621 ggaggagcga gatgtcgaag gacaagcaga ccagtccgcg cgctgcgagc gccgcctcga
     1681 aggtattgcg agatgggcgc acgggcgccg cctcgaagac cgccgcgggc agcgcgttgg
     1741 cgcagacccc cgcgaagccg tcgaagaagc ggtcatgacc gccgctcgac gacaaccagg
     1801 atcggctaca caaaggagac agccatgaca cagaacagtg ttgccgtagg gaaacgcatc
     1861 aaagaagtgc gcgaagcgcg caaggcgacg gacccgcgct tttcgttgcg acggttcgcc
     1921 gacgcggtgg gcttgagtcc caccttcatc agtcgcttgg aacacggtac gggcgtgttg
     1981 cccagcccgg ccaatctcga acgcatcgcc gaggtgctgg agattgaggc caaagaactc
     2041 ttcgagctcg cgcaacgcgt cgagccccag gtcaaggatt tgttgctgga gcaaccggcc
     2101 tgggcggatt ttttacggac ggcacggtcc cgcggatggg acgcagatgc cgtcaagcag
     2161 tttgtggaag accactatac ggagccggac accgacgcat gaaacgcaca cgctttcttc
     2221 cagaacagga tatcgaacgg gcggccttgg tcctggccga acggtattgc catcgcttca
     2281 gtcagccgct gaccccgcat cttcccttgg aggagctgat cgagtgcgtg ctggagttgt
     2341 ccttccgcgt cgacgatttg accgcgttgt tcgggacggc cgatatcctc ggcgcgacca
     2401 aggttgacga gcggctcatt ctcgtggata tgtcattgga cccctgtgag catcccgaaa
     2461 aagaagggcg ctatcgtttc acgttggcgc atgagatcgg gcattgggaa ctgcaccggc
     2521 aatcggtgcc cacggcggcg caacggacct tgttcgatac cgacccgctc gggacgatcc
     2581 tctgccgcgc ctcgcagtat gatc
//
LOCUS       DAOSRB010000300         2534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_37273_length_2534_cov_9.420734, whole genome shotgun sequence.
ACCESSION   DAOSRB010000300 DAOSRB010000000
VERSION     DAOSRB010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37273_length_2534_cov_9.420734"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLZ74_11040"
     CDS             complement(<1..305)
                     /locus_tag="PLZ74_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOR98895.1"
                     /translation="MARKTKAEAEKTRQCILKAALRLFVEKGYDRTTFADVAQRIGLT
                     KGAVYWHFKSKPDLLAELVVLMTERHTTQLARALPHPVSLDTPEALTENFVLRARLIV
                     "
     gene            675..1625
                     /locus_tag="PLZ74_11045"
     CDS             675..1625
                     /locus_tag="PLZ74_11045"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR98896.1"
                     /translation="MAKKTRTLYRRMRRPLEWCAVWVALAVIPSLSLRRLLGLSRWIA
                     DIGLLFDYRGKTIARANLALMFGPRMTPYRERVIIRHAYRNMARVLVNVFWLSRDTRT
                     RVLDQVAFVPSVLETLRAAHPAITVSAHFGNWEILSQACVAHGVPMMSVAKEIGSPEM
                     TAQLIRLRSTIGQEIVPAEGAIRPLIQALRKGKSIGLLIDQHTHLWEGGAWISFFGVP
                     AGISLTPAALALKLNRPVLFAWSRPLHDGRYRIEPGELFLPEPGLDAATLTQRIAAAF
                     ERVIRRHPSFWCLNYRRWRYIRSGDDPTRYPDYARPERIR"
     gene            complement(1656..>2534)
                     /locus_tag="PLZ74_11050"
     CDS             complement(1656..>2534)
                     /locus_tag="PLZ74_11050"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98897.1"
                     /translation="RAMVRAVERSGITFQTGAQSRSNDYFRMACELVRNNRIGRLQRV
                     EVGLNKGHSTFGKTPEQLSETPLATPPPYVDFDLWLGPAPARKFTPKIHHPMQWRYNL
                     EYSCGMLTDFAAHQLDIMQWALGKDGIEPVAIENIKGRLPPSTDLYNTLAEPFSFEIV
                     YGDGTRVFVSSEYTYGCRFYGENGKQIYVTNGKIETNPPELVREKIGENEVRLIRSGQ
                     QEKNFIEAVYSGATPISPVQAGFSALVPAVLAHIGMRTERASLKWLAAKTRFENAPDA
                     DKLLAPHMRAPWKLDV"
BASE COUNT          448 a          843 c          736 g          507 t
ORIGIN      
        1 acaatcaggc gggcgcgtag aacgaaattt tcggtcaacg cctccggtgt atccagcgac
       61 accggatgcg gcagcgcacg cgccaactgg gtcgtatgcc gctccgtcat cagcacaacg
      121 agctcggcga gcaagtcagg cttgctctta aaatgccaat agaccgcgcc ttttgtcagg
      181 ccgatacgct gcgccacgtc cgcaaaagtt gtgcggtcat aacctttttc gacaaacagc
      241 ctgagcgcgg ccttgagaat acactgccgc gttttttctg cttccgcttt ggttttccgc
      301 gccatgcgca cccctccctt cgcgaaatag gcgcacacta tacatacctc cgggtaggta
      361 tgcaagactg attccgaccc tgacgccgat tcgggcgcat ccctgtttcc ctgcttgacg
      421 ccacgccctg aagagcgagc gtccccacga gccgcagacg gtccctgccg gtgcggcggg
      481 acgtccgcag ccatgcgcca tcagcacgga gccggaccgc cgaaaccgtg ccgcctccgg
      541 gcaaggccgc atccccggga aaccggatcg tgcgcggaaa agtttctcca tgcgaaaaca
      601 aatcattaca acacggcaag accgtttttt ttgcctttcc cgtactgccc cggcttgcta
      661 tgctaaaatt atctatggca aagaaaacac gtacgcttta ccgacgtatg cggcggccgc
      721 tggagtggtg tgccgtatgg gtggcgttgg ccgtgattcc aagcctttcg ttgcgccggc
      781 tgctcgggct ctcccgctgg attgcagaca tcggtttgct gttcgattac cgcggcaaga
      841 cgatcgcccg agcgaatttg gcgctcatgt tcggaccccg catgacacca taccgcgaac
      901 gcgtgatcat ccgccacgct taccgcaaca tggcccgggt gttggtgaat gtcttctggc
      961 tctcccgtga cacgcgcacc cgtgtgttgg atcaggtcgc gttcgttccg tccgtactgg
     1021 agactctacg cgcggcgcac cccgccatca cggtgagtgc ccacttcggc aactgggaga
     1081 tcctctccca agcctgcgtg gcgcatggcg tcccgatgat gtcggtcgcc aaagagatcg
     1141 gctctcccga gatgaccgcc caactgatcc gcctgcgctc gaccatcggc caggagatcg
     1201 tacccgcgga aggcgcgatc cgtccgctga tccaggcgct gcgcaagggc aaatcgatcg
     1261 gattgctgat tgaccagcac acccatcttt gggagggcgg tgcctggatc tcgtttttcg
     1321 gcgtcccggc gggcatctcc ctgacgcccg ccgccctggc gctcaagctg aaccgtccgg
     1381 tgctcttcgc ctggtcccgt ccgctgcatg acggccgcta ccgcatcgag ccgggagagt
     1441 tgttcctgcc cgaacccggc ctcgatgccg caaccctcac ccagcggatc gccgccgcgt
     1501 tcgagcgggt gatccgccga cacccctcct tctggtgtct caactaccgc cggtggcggt
     1561 acatccggag cggcgacgac cccacgcgct atccggacta cgcgcggccg gagcgcatcc
     1621 gttaagcgcg ccccggcccc gcgtcacgcg gcccgtcaca catccaactt ccacggggcg
     1681 cgcatgtgcg gcgcgaggag tttgtccgca tcgggagcat tctcgaagcg cgttttcgcg
     1741 gccagccatt tgagtgaagc cctttcggtc cgcatgccga tgtgcgccag cacggcaggc
     1801 accaacgccg agaaacccgc ctggaccggc gaaatcggcg tcgcgccgct gtacaccgct
     1861 tcgatgaagt tcttctcctg ttgtccgcta cggatcaacc ggacttcgtt ttccccgatc
     1921 ttctctcgga ccagctcggg cggattggtt tcgatcttgc cgttggtcac gtaaatctgc
     1981 ttgccgttct cgccatagaa acggcaaccg taggtatatt ccgacgagac aaagacgcgg
     2041 gtgccgtctc cgtaaacaat ctcaaatgag aacggttccg ccagcgtgtt gtagaggtcg
     2101 gtggacggcg gcaaacgccc cttgatgttt tcgatcgcga ccggttcgat gccatccttg
     2161 cccagcgccc actgcatgat atcaagctga tgcgcggcga agtccgtcag catgccgcac
     2221 gaatactcca gattgtagcg ccactgcatc ggatggtgga ttttgggcgt gaattttctt
     2281 gccggcgcgg gtcccagcca caggtcgaaa tcgacatagg gcggcggcgt ggcgagcggc
     2341 gtttcgctca actgctccgg cgtcttgccg aacgtggagt ggcctttgtt caacccgacc
     2401 tcgacgcgct gcaagcggcc gatccggttg ttgcgcacca gctcgcacgc catgcggaag
     2461 taatcgttcg agcgcgactg ggcaccggtc tggaacgtga tgccgctgcg ctcgaccgcc
     2521 cgcaccatgg cgcg
//
LOCUS       DAOSRB010000301         2530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_37362_length_2530_cov_5.269495, whole genome shotgun sequence.
ACCESSION   DAOSRB010000301 DAOSRB010000000
VERSION     DAOSRB010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37362_length_2530_cov_5.269495"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            664..1416
                     /locus_tag="PLZ74_11055"
     CDS             664..1416
                     /locus_tag="PLZ74_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98898.1"
                     /translation="MVEHWNDSGIASGSDFILAWLKSLFATPKPTEPVTGMKVLNMMH
                     VVLIASIVGMTFYASKLEPSNKPPAIMVWFEDNGIGKVSSPQLVAAVWDNGDMLWSTN
                     TLCGGTPYLYGKVCGAINVGKVIDYFKHKGYFSAVYSRHNMGPDSKHIGMFISEGTNA
                     LLTRSWHEIMEQNPNLVALSHGITSLHGRNRETVLEKDKAEYLQYRAMWTDVRSQINA
                     MIPQKGCVIEDVRVQRRRGKHPPTIIWKQGSL"
     gene            1486..>2530
                     /locus_tag="PLZ74_11060"
     CDS             1486..>2530
                     /locus_tag="PLZ74_11060"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOR98899.1"
                     /translation="METVKSRVGVFWRCPSCSGRSATIALLRKSVPAKVVNAVWLAAR
                     SGAFPAIHTCPACMGRMAEVPADEKRLFIDICTRCQFVWFDIGEEEAMPKIPRTPSWE
                     ETLPLETRQRLAILEIERIREARELESSDSMPDVWWQWIPGLLGLPIEQGASICRSRP
                     WVTWTLGTLIVMVGLATLGDLPAAIQQYGLVPAQAGRLGGLTFVSSFFLHGGIAHLLG
                     NLYFLLIFGDNVEDWLGWKRFLLLIVGASLLGNIVHILADPSSTVPCVGASGGISGIL
                     AFYASKFPNVRLKLLFRHLVFIRWLSISAIAAFGVWIALQLFGVLAQVSGFSNVSALA
                     HLGGASAGLICCIF"
BASE COUNT          558 a          694 c          720 g          558 t
ORIGIN      
        1 tcgacaagtt ccacaccgaa ccagcccgcc gcaaaaccac ccgccaccac gccgccaaga
       61 ccaagaaacg ccttgcgcgt catacgtgcg gaagcggtcg attcatttct ttttttcatc
      121 ataccctact ttccttcgaa aaatccttga ttgtcgcgtc gttcagtgcg gcggcaggac
      181 ggcgcgaaaa agcggtgccg cgacccgcgc gcaacgcggc tcttgtcacc gcacatcgcc
      241 atcatcttac cggaagtttg cggccaaaag caatcgacca aaacacttcg tcgtttttac
      301 acttcgtccg aacacttcgt caccaacgca atcgactcgg tgtagagacg aagtgtgtgt
      361 ccctgcgcac agcgcggagc acggtggcgt gaccgccgcg gacgagcaga cgctcgtccc
      421 tccccgcctg cggtcaaaaa caaaaaagaa aaaatctgcg caatctgcgc aaatctgcgg
      481 ataaaaccag gaaccgcaag acacgctgtt gcgggcgggg acgcccgcgc tcccgactct
      541 ctgctccctt ttcgtcggga atgcatgatc ttcgcataac ccctgtcacg aaccgattac
      601 caacaatttt ttgcgtgtgg ttgacgccct caccttaacc gcgtaccatg accgcgactg
      661 tcgatggtag agcactggaa cgattccggc atcgctagtg gaagtgattt tatactggca
      721 tggctcaaat cgctgtttgc gactcctaaa ccaacagaac cagtgacagg aatgaaggtg
      781 ttaaacatga tgcacgtcgt tttgattgcg tcaatcgtcg ggatgacttt ttacgcaagt
      841 aaacttgagc cttcaaacaa accgccggcc attatggttt ggtttgaaga taatggcata
      901 gggaaagtgt caagcccaca gcttgttgcc gctgtgtggg acaacgggga catgctgtgg
      961 tcaacgaata ccctctgcgg cgggacacct tatctatatg gcaaggtttg tggcgcaatt
     1021 aacgttggca aggtgatcga ttacttcaag cacaaaggat atttttctgc tgtatattcg
     1081 cgccataaca tggggccgga ctcgaagcac atcgggatgt ttatctcgga gggaacgaat
     1141 gctttgctca cgcgatcatg gcatgaaatc atggaacaaa atccgaacct cgtcgcactc
     1201 tctcacggga tcacgtcact gcacggacga aatcgggaga ccgtcctaga gaaggacaaa
     1261 gcggaatact tacaataccg tgccatgtgg acagacgtac ggtcacaaat caacgccatg
     1321 ataccgcaaa aaggttgtgt cattgaggat gtgagggtac agaggcgtag gggaaaacat
     1381 ccccctacaa taatctggaa gcaagggagt ctgtaacggt tcgcgtaaaa ggcgggcaga
     1441 cggtggggcg cgggcatgta tagttgtcca aaatgcaagg ttgcgatgga gacggtcaaa
     1501 agccgggtgg gtgtgttttg gcgatgcccg tcctgttcgg gccgatccgc cacgatcgcc
     1561 ttgcttcgga agagcgtccc ggcaaaggtc gtgaacgcgg tctggctggc tgcacggtct
     1621 ggagcctttc ctgcgatcca tacgtgtccc gcctgtatgg gccgcatggc cgaggtgcct
     1681 gccgacgaaa agcgactttt tatcgacatc tgcacccggt gccagttcgt ctggttcgat
     1741 atcggcgaag aggaggcaat gcccaagatc ccccgcacgc cttcctggga ggagacgctc
     1801 ccgctggaga cgcgccagcg gctggcaatc ttggagatcg aacgcatccg agaggcaagg
     1861 gagttggaga gcagcgacag catgcccgac gtctggtggc agtggatacc cggcttgctc
     1921 gggcttccca tcgaacaagg ggccagtata tgccggagcc ggccatgggt gacgtggaca
     1981 ctggggacac tcatcgtgat ggtagggctc gcaacactgg gagacctccc ggccgccatt
     2041 cagcaatacg gtctcgtgcc ggcacaggcg ggccgccttg gcgggctgac gttcgtgtcc
     2101 tcattcttcc tgcacggtgg catcgcgcat ctgctgggca acttgtattt cctcctgatc
     2161 tttggagaca acgtggaaga ctggctaggc tggaaacgtt ttttgctcct gattgttggc
     2221 gcgtcgctac ttggcaacat cgtgcacatt ctcgcggacc cgagttctac ggttccttgt
     2281 gttggcgcga gcggcggcat ttcgggaatt cttgcattct acgcatcgaa gtttccgaat
     2341 gtgaggctca agctactctt tcggcaccta gtgtttatcc gttggctgtc catatccgca
     2401 atcgcggcgt ttggggtctg gatcgcgcta cagttgttcg gcgtccttgc gcaagtgtcc
     2461 ggcttcagca acgtgtccgc gctggcgcat ctgggcggcg cgagtgcagg cttgatatgc
     2521 tgcatatttc
//
LOCUS       DAOSRB010000302         2529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_37388_length_2529_cov_3.523040, whole genome shotgun sequence.
ACCESSION   DAOSRB010000302 DAOSRB010000000
VERSION     DAOSRB010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37388_length_2529_cov_3.523040"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PLZ74_11065"
     CDS             complement(<1..1090)
                     /locus_tag="PLZ74_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98900.1"
                     /translation="MASRIIQNNFVAGEISPELHGRHDIKAYFQGAAKLENFVPRRTG
                     GVRKRAGTQFMLKLHDDSATKFRAFPFFFDRTTFCELILFKKTGDAKLYYALHKRDAD
                     GGETTVGTDDYTTVTSLGADSDLDDLNYKQIGDTLFFTAAGRRAFKAEIFLSGMRVEW
                     EQLSGTITPAAPPALNSTATGFKPSTDEGYVNSSRKYALYGMKSGVFSAPLEKDVTIS
                     LPWISGARVNLSFTPRWDQHDYYILGRLQGANYGVVSKFFPNLQSGSNADSTFADTNA
                     EETGTVGGVGYTAGSDGASVAWKRNPDDTNAAASDNAWHVNAKFVTGKLTATYKSASH
                     PILSLRIWFGAKVRKTSDRPGTDTVAAIG"
     gene            complement(1095..1664)
                     /locus_tag="PLZ74_11070"
     CDS             complement(1095..1664)
                     /locus_tag="PLZ74_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98901.1"
                     /translation="MTKLEIWNGALAQLPHDRTVASLTETSTEALRCAQEWEAARVKV
                     LCAHEWGFLRMSVPVCAGVWCGEVGGMLYAYPRPTSALRILGLFDAEGRKVRAEVVNG
                     LLVSPEPTASVRFLYDEADPEVWPSLVQRAVLMELAARLCPVITMNMVNTRVLMERAK
                     MALDDAMLADSSEVEYSGTAGDTYIMARI"
     gene            complement(1696..2073)
                     /locus_tag="PLZ74_11075"
     CDS             complement(1696..2073)
                     /locus_tag="PLZ74_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98902.1"
                     /translation="MKKKITAFMLVAALATAGLGGGLSLLTFELSLPGGATNEVSLTY
                     PGRASVVAPVLAFAPDTNGVITVKYYSAAVPDGFAVGGFASGSRTFDMASFPPIHYGE
                     SYKFTSSVTNAGTVLVTGEIANP"
     gene            complement(2221..>2529)
                     /locus_tag="PLZ74_11080"
     CDS             complement(2221..>2529)
                     /locus_tag="PLZ74_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98903.1"
                     /translation="IYYRVKNGVVFFSGSFVAIVQSPGKPLTTSSLPEGITPGDTYNT
                     DSILSADCGVWYLPHVWFSRSERRPYLGGVIYLGNGSKVPVNTSVAIHFRGDASFVIG
                     "
BASE COUNT          489 a          827 c          725 g          488 t
ORIGIN      
        1 cgccgattgc cgcgacggta tcggttcccg gacgatcgct ggtcttcctg accttcgcgc
       61 cgaaccaaat ccgcagggac aggatcggat gcgacgcgct cttgtacgtc gccgtgagct
      121 tgcccgtgac gaacttcgcg ttgacgtgcc acgcattatc cgaagccgcc gcgttcgtgt
      181 cgtcagggtt gcgcttccag gcgaccgacg cgccgtcgga acctgccgtg tagccgacgc
      241 cgccgaccgt cccggtctcc tccgcgttcg tgtccgcgaa cgtgctgtct gcgttcgaac
      301 cgctctggag gttcgggaag aacttgctca cgacgccgta gttcgcgcct tgtaggcgac
      361 cgagaatgta gtagtcgtgc tgatcccagc gcggcgtgaa cgagaggttg acgcgagccc
      421 cggatatcca cggaagcgag atagtcacgt ccttttcgag cggcgcggag aacactccgc
      481 ttttcatccc gtagagcgcg tacttccggg aactattgac gtagccctca tccgtcgagg
      541 gcttgaaccc cgtcgcagtg gagttgagcg ctggaggcgc ggctggcgtg atcgtgccgg
      601 agagctgctc ccactcgacg cgcatcccgg acaggaatat ctcggccttg aacgcccgcc
      661 gccccgccgc cgtgaagaac agcgtgtcgc cgatctgctt atagttgaga tcgtctaggt
      721 cggaatccgc ccctaggctc gtcaccgtcg tgtagtcgtc cgttccgacg gttgtctcgc
      781 caccgtccgc gtcgcgcttg tgcaaggcgt agtagagctt ggcgtcacca gtcttcttga
      841 agaggatcag ctcgcagaaa gtagtgcggt cgaagaagaa cgggaacgcc cggaacttcg
      901 tggcgctgtc gtcgtgtagc ttgagcatga actgcgtccc ggcccgcttg cgaaccccgc
      961 ctgttctacg tggaacaaag ttctcgagct tcgcagcgcc ttggaagtac gccttgatgt
     1021 cgtgccgccc atgcagctca ggcgaaatct cgccagccac gaagttgttc tggatgatcc
     1081 gcgacgccat gaagctatat cctcgccatg atgtacgtgt cgcccgccgt cccggagtac
     1141 tcgacctcgc tggagtccgc tagcatcgcg tcgtcaagcg ccatcttcgc ccgctccatg
     1201 agaaccctgg tgttcaccat gttcattgtg attacggggc agagacgagc cgcgagctcc
     1261 atgagaaccg cccgctgcac aagcgacggc cacacctccg ggtcggcctc gtcgtagagg
     1321 aagcgcaccg acgccgtcgg ctcaggcgac accagaagcc cgttgacgac ctccgccctg
     1381 accttgcgcc cttccgcgtc gaacagcccg aggatgcgca gggccgacgt cggtcgggga
     1441 tacgcgtaga gcatcccccc gacttcgccg caccaaacac cggcgcacac gggaaccgac
     1501 atgcgaagaa acccccactc gtgggcgcag agcaccttca cgcgcgccgc ctcccactcc
     1561 tgggcgcacc gcaacgcctc cgtggaggtt tcggtcagcg acgcgaccgt gcggtcgtgc
     1621 ggaagctgcg cgagcgcccc gttccagatt tcaagtttcg tcatgtgtca agccctcccg
     1681 tgcgcaccgg ccagctcagg gattggcgat ctcgccagtc accagcaccg tcccagcgtt
     1741 cgtcacgctg ctcgtgaact tgtagctctc cccgtagtgg atcggaggga acgacgccat
     1801 gtcgaacgtc cggctgccgc tggcgaaccc gccgaccgcg aacccgtcag gaacggcggc
     1861 ggaatagtac ttgaccgtga tgacgccgtt cgtgtccggc gcgaacgcca gcaccggagc
     1921 cacgaccgaa gcgcggcccg gatatgtcag cgaaacctcg ttcgtcgcac cgcccggaag
     1981 cgaaagctcg aacgttaaca gcgagaggcc gccgccaaga cccgccgtgg ccagcgccgc
     2041 gacaagcatg aatgccgtga tcttcttctt catcttttca tttctccttc ttgttttgca
     2101 tggcagtccc gacgcgggaa tgccaaagtg gaaaacgcga tcatcctccg aaaacgcact
     2161 tgatgaactg ctcgcgaaac acgatcaatg tccaccagta ccagtacatt tccaaacctc
     2221 ctatccgatc acaaatgatg cgtcacccct gaaatggatt gcaacggatg tattcaccgg
     2281 gactttgctg ccattgccca agtagatgac cccgcccaaa tatggccttc gttcagatct
     2341 ggagaaccaa acgtgtggca gataccagac accgcagtcc gccgacaata tggaatcagt
     2401 attgtatgta tcgcctggcg tgatcccttc cggcagggac gacgtcgtca atggcttgcc
     2461 tgggctttgc actattgcga cgaaacttcc gctgaaaaac accacgccgt tttttacgcg
     2521 ataataaat
//
LOCUS       DAOSRB010000303         2525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_37470_length_2525_cov_5.530769, whole genome shotgun sequence.
ACCESSION   DAOSRB010000303 DAOSRB010000000
VERSION     DAOSRB010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37470_length_2525_cov_5.530769"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /gene="folK"
                     /locus_tag="PLZ74_11085"
     CDS             complement(<1..473)
                     /gene="folK"
                     /locus_tag="PLZ74_11085"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOR98904.1"
                     /translation="MATPREAILSLGSNLGDRRIALERACAALEEEPGLRILERSPIY
                     ETEPVGVPEEEAGRLFLNQVVIVSTDRSPEALSDTVHAIEARWGRTRTGVHGAARVLD
                     IDLIAFGTERRNTPELTLPHPRTRERRFVLQPLADLRPNLVLPGESRSVAELLRDL"
     gene            complement(491..1300)
                     /gene="dapB"
                     /locus_tag="PLZ74_11090"
     CDS             complement(491..1300)
                     /gene="dapB"
                     /locus_tag="PLZ74_11090"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOR98905.1"
                     /translation="MKIAILGAAGRMGQNLLRCAEKIGACQIVAATECPGHPLLGQSV
                     RQAFGLTAENIPETLRIEETWPKDTEVVIDFTFHTTVTNNLARAIACRQAYVLGTTGF
                     TEAERQTILAATQQIPMVWAPNMSLGVNLLFELVRRAASVLGPEYDAEIVEIHHRRKK
                     DAPSGTALGLAEALARGRNVKLDEVACYGRQGIPGERPEGEIAIHALRGGGVVGDHTV
                     LFIADEERIELTHRANSRESFAAGALRAALWLEGRPAGLYTMRHVLGFESV"
     gene            complement(1357..2226)
                     /gene="dapA"
                     /locus_tag="PLZ74_11095"
     CDS             complement(1357..2226)
                     /gene="dapA"
                     /locus_tag="PLZ74_11095"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOR98906.1"
                     /translation="MTLTGTFTALVTPFNTDGSVDYPALHALVQWQIENGVTGLVPVG
                     TTGESPTLEIDEHIRVITATAEAAAGRAVIIAGTGANATAEAIELTRQVLNAGVQATL
                     QVTPYYNKPNAEGLYRHFAAVADLGLPVVLYNVPGRAGREIPLDVVIRLAAHPNVAAI
                     KEAAGSVDRVSAIRNACELPVLSGDDSLALPMISVGACGVISVASNVIPKEMSKLVQL
                     ALANDLVEARALHQTYYPLFRDLFIDTNPIPVKTALTLMNRVGPTFRLPLCEPSEKIR
                     AQLVQTLTHLNLM"
BASE COUNT          434 a          786 c          823 g          482 t
ORIGIN      
        1 agatcgcgca gtaattccgc aacgcttcgg gactcgccgg gcaggacgag attgggcctc
       61 aggtctgcga gcggctggag cacgaagcgg cgttcacggg tgcgcgggtg cggcagcgtc
      121 agttccggcg tgttccggcg ctccgtgccg aaggcgatca ggtcgatgtc cagcacgcgc
      181 gccgcgccgt gcacaccggt tcgtgtccgg ccccagcgcg cctcgatcgc atgcaccgtg
      241 tcggaaagcg cctcggggga acggtcagtc gaaacgatca ccacctgatt gaggaaaagt
      301 cttccggcct cttcttccgg cacgccgacc ggctcggtct cgtagatcgg ggaacgctcc
      361 agaatccgga ggcccggctc ctcttccagc gccgcgcacg cccgctccaa cgcgatgcgg
      421 cggtcgccga gattgctgcc gagagacaga atggcttcgc gtggggttgc catggggggg
      481 gaacgcggcg tcacacggat tcgaatccga gcacatgccg catggtgtaa aggcctgcgg
      541 gacggccttc gagccagagg gcggcgcgca gggcgcctgc ggcgaaggat tcccggctgt
      601 tcgcccggtg cgtgagttcg atgcgttcct cgtcggcgat gaagagcacg gtgtggtcgc
      661 cgaccacgcc cccgccgcgt agggcgtgga tcgcgatctc gccttccggg cgttcgccgg
      721 gaattccctg gcgtccgtag caggcgacct catcgagttt cacgttgcgg ccccgggcca
      781 gtgcctcggc gagcccgagt gccgtgccgc tgggcgcgtc cttcttgcgg cggtggtgga
      841 tttccacgat ctccgcgtcg tactccggcc ccagcaccga cgccgcgcgc cgcaccagtt
      901 caaacagcag gttcacaccc agactcatgt tgggtgccca aaccatgggg atttgctgtg
      961 tggcggcgag gatcgtctgc cgctcggctt ccgtaaaccc cgtagtgccg agcacgtacg
     1021 cctgacggca ggcgatcgcg cgggcgaggt tgttggtcac cgttgtatgg aacgtgaaat
     1081 cgattaccac ttccgtgtcc tttggccacg tttcctcgat gcgcagggtc tctggaatgt
     1141 tctccgccgt tagaccgaag gcctgacgca ccgattggcc cagcaacggg tggccggggc
     1201 attcggtggc ggcaacgatt tggcaggcac cgatcttttc cgcgcatcgc agcaagttct
     1261 gtcccatgcg gcctgcggct ccgagaatcg caatcttcat atcaggatcg tcccttcgtc
     1321 tggttggtgg ggaatgacgc cggccggatc gggccgttac atcaggttga ggtgggtcag
     1381 ggtttgcacg agttgcgcac ggatcttttc cgacggttcg cacagcggca gacggaaagt
     1441 cggccccaca cgattcatca gcgtcagcgc ggtcttgacc gggatcgggt tggtatcgat
     1501 gaacaggtca cgaaaaagcg gatagtaggt ctggtgcagc gcgcgcgctt caaccagatc
     1561 attggcgagg gcgagctgca cgagctttga catctccttc gggatcacat tcgaggccac
     1621 gctgatcacg ccgcacgcgc ccacgctgat catgggcagc gccagactgt cgtcacccga
     1681 gagcaccggc aactcgcagg cgtttcggat ggcgctgacg cggtcgacgc tgcctgcggc
     1741 ttccttgatg gcagccacat tcggatgcgc ggcgaggcgg atcaccacat ccagcgggat
     1801 ctcgcgtccc gcgcggcccg gcacgttgta caacaccacc ggcagcccca gatccgccac
     1861 cgcggcgaaa tggcggtaga gcccctcggc gttgggtttg ttgtagtaag gcgtcacttg
     1921 caacgtagcc tgaacacccg cgttcagcac ttggcgcgtg agttcgatcg cctcggctgt
     1981 ggcattggcg ccggtgcccg caatgatcac tgcgcgtccc gcagcagcct cggccgtggc
     2041 ggtgatgaca cggatgtgct catcgatctc cagggtgggc gattcaccag tggtgccgac
     2101 cggcacgaga ccggtgaccc cgttctcgat ctgccattgc accaaggcgt gcagcgcggg
     2161 ataatccaca ctgccatccg tgttaaaagg ggtgaccaaa gcggtgaatg tgcctgtcag
     2221 cgtcataatc tcctcaagcg tcgattgcgt gcgaccagta tgcggaaacg ggaaggggag
     2281 gtcaaccccc tttgttgagt ttggcagcga tctcggcagg tttccggtat cggcggggca
     2341 cgggcccaaa ccgcgtccgg aaccaatcca caataccaaa accgggtcac tttgcgcatt
     2401 gacaactttc cgccaatccg atacattttg cgcactttgt tcccttgcgg gataattgtg
     2461 ccgaacgaca attccggcgt agcgcagcgg tagcgcagtt ggctgttaac caattggtcg
     2521 ctggt
//
LOCUS       DAOSRB010000304         2519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_37583_length_2519_cov_6.357549, whole genome shotgun sequence.
ACCESSION   DAOSRB010000304 DAOSRB010000000
VERSION     DAOSRB010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37583_length_2519_cov_6.357549"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..842)
                     /locus_tag="PLZ74_11100"
     CDS             complement(78..842)
                     /locus_tag="PLZ74_11100"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOR98907.1"
                     /translation="MAQAPTLSDIAYRAITRKLMASELLAGQKLSEQKIAAECGISRT
                     PVREAVRRLIEEGVLYQIPCSGTYVARLDRRQLIDAYEVRMALECFALDRAVRNLTRE
                     QRAECRQTCDVMRKVAEGLRKRNKTVLDGKPLVTFLKADLSFHLLLLKAAGNALAIKI
                     VTHAYQRNLFFGHYSHRRDLNHVAWAWHHHAEIERAIRRGDADRATRWMRTHIARSRE
                     DALIAFDQAQISLIHANHPPRDPVHEALDRFASQLV"
     gene            complement(986..>2519)
                     /locus_tag="PLZ74_11105"
     CDS             complement(986..>2519)
                     /locus_tag="PLZ74_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98908.1"
                     /translation="ASDAVLSGGVRRSATICLFSPDDELMATAKTGNWFNENPQRARS
                     NNSAVLLRNQTTREQFNRLMKSVKEFGEPGFVWTDHLDMTFNPCGEIGLYPQIDGVTG
                     FAFCNLCEINMGLVDNADKFREACRAAAILGTLQADYTDFEYLGEVSTRIAKREALLG
                     ISMTGMMENPDVSFDPQLQRDMAKVILEVNAEVAARIGINPAARTTCVKPAGTTSSIL
                     GTSSGIHPHHAKRYFRRAQANEDELLVDFFGAQNPKAVEKSVWNPNGTDVVLTFCVEA
                     SPHALTKRDVDAVELLNKVKLTKENWIDAGKRPEQCVQPWLSHNVSNTISVKDSEWES
                     VADFIYENRHVFAGVSLLPDGGDLDYPQAPFCEVLTADEIVEIYGVGALLASGLIVDG
                     LHAFDDNLWAACDCVLGRGENLEEPTPKTYPKRVYEAIEGVREAKQDWVRRARKFARN
                     YFGNDVLKMTRCLKRVNACKHWEDLTRTYAPVDYTQLIERQDNTTVTQTVACTGGKCE
                     IL"
BASE COUNT          448 a          712 c          817 g          542 t
ORIGIN      
        1 gaaaaagcgc gggcatggct cagagacaac gggcaactcc tcgaagaaga catctgagca
       61 atgacatgcg gtgtgtctca aacgagttgg gaagcgaagc ggtcaagggc ttcgtggacc
      121 gggtcccgcg gcgggtggtt tgcgtgaatc agggatatct gggcttggtc gaaggcgatc
      181 agcgcgtcct cccggcttcg ggcgatgtgg gtgcgcatcc agcgggttgc gcgatcggcg
      241 tctccgcgcc ggatggcgcg ctcaatctca gcgtgatggt gccatgccca ggcgacgtga
      301 ttgagatcgc gccgatggct gtagtggccg aagaagaggt tccgctgata ggcgtgggtc
      361 acgatcttga tggccagcgc gtttccggcg gctttgagca acagcaggtg gaacgagagg
      421 tcggccttga ggaaggtgac gagcggtttt ccgtccagaa cggttttgtt tcgcttacgc
      481 aacccttccg cgactttccg catgacatcg caagtttgac ggcattcggc gcgctgttca
      541 cgggtcaggt tcctgaccgc gcggtcaagg gcgaagcatt caagggccat gcggacttca
      601 tacgcgtcga tcagttgacg gcggtcgagc cgggcgacat acgtgccgct gcaggggatt
      661 tggtagagga ccccttcctc gatgaggcga cgcacggcct cgcgcaccgg ggtccggctg
      721 atcccgcact cggcagcgat cttctgttcc gacagtttct gtccggcgag cagttcggaa
      781 gccataagct tacgggtgat ggcccggtat gcgatatcac tcaatgtggg tgcttgtgcc
      841 acacgatcct cgttgtttgc catgcgtccg ggttgacgga cgcgatggcg ttattgatat
      901 cacaaatcgc cgggggtgtc agccatcacg atccggttcc cgggcagcga ccgaaaaatg
      961 cgggaccggc gggatgcggc gcggatcaga ggatttcgca tttgccgccg gtgcaggcca
     1021 cggtctgggt gaccgtggtg ttgtcctggc gctcgatgag ctgggtatag tccaccggcg
     1081 cataggtgcg tgtgaggtcc tcccagtgtt tgcacgcatt gacgcgtttg aggcaacggg
     1141 tcattttcag cacatcgttc ccgaaatagt tccgggcgaa cttgcgcgcg cggcgcaccc
     1201 aatcctgttt ggcctcgcgc acgccttcga tcgcctcgta gacgcgtttg gggtaggtct
     1261 tcggtgtggg ctcctccaga ttttccccgc gccccaagac acagtcgcag gcggcccaca
     1321 ggttatcatc gaacgcatgc aggccgtcca cgatcaggcc ggaggcgagc aacgcgccga
     1381 caccgtagat ttccacaatc tcatcggcgg tgagcacttc gcagaagggg gcctgcgggt
     1441 agtcgaggtc tcccccgtcc ggcagcagcg agacccccgc gaacacgtgg cggttttcgt
     1501 agatgaaatc ggcgacactc tcccattcgc tgtctttgac ggaaatggta ttggagacgt
     1561 tgtggctgag ccagggttga acacactgtt cagggcgttt gcccgcatcg atccagtttt
     1621 ctttggtgag cttgaccttg ttcagcaact ccacggcgtc cacatcccgc ttggtgagcg
     1681 cgtgcggact tgcctccacg cagaatgtca gcaccacatc cgtgccgttg ggattccaga
     1741 ccgatttttc cactgctttg gggttctgcg cgccaaagaa atcaacgagc agttcatcct
     1801 cgttggcctg ggcgcgccgg aaataacgtt tcgcatggtg ggggtggatc cccgacgatg
     1861 tgcccagaat gctgctggtg gtgccagcgg gtttgacaca ggtggttcgt gcggcggggt
     1921 tgatgccgat ccgtgcggcc acctccgcat tgacttccag aatcaccttt gccatgtctc
     1981 gctggagctg cggatcaaac gagacgtccg ggttttccat catgccggtc atcgaaatgc
     2041 cgagcagcgc ctcacgtttc gcaatgcgcg tgctgacctc gccgagatat tcgaagtcgg
     2101 tatagtccgc ttgcagggtt cccagaatgg cggccgcgcg gcaggcttcg cggaatttgt
     2161 ccgcgttgtc caccagcccc atgttgatct cgcacaggtt gcaaaaggcg aatcccgtga
     2221 cgccgtcgat ctgcggatag agtccgatct ctccgcaggg gttgaaggtc atgtcgagat
     2281 ggtcggtcca aacgaatccg ggctcgccga actccttgac cgatttcatc agccggttga
     2341 actgttcgcg ggtggtttgg ttacgcagca ggacggcgga attgttggac cgggcgcgct
     2401 gcgggttttc attgaaccag tttccggtct tggccgtggc catcagttcg tcgtccggcg
     2461 aaaagaggca gatggtggcg ctgcgtcgca cgccgcccga aaggaccgca tccgatgca
//
LOCUS       DAOSRB010000305         2493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_38135_length_2493_cov_6.679655, whole genome shotgun sequence.
ACCESSION   DAOSRB010000305 DAOSRB010000000
VERSION     DAOSRB010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38135_length_2493_cov_6.679655"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..956
                     /locus_tag="PLZ74_11110"
     CDS             171..956
                     /locus_tag="PLZ74_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98909.1"
                     /translation="MRVLKRWWLRSRAFRAAQPELEACRATMERLFGGPVTWRRTGGC
                     GRDVVCLVSCGGKPVGVLRRVAPVAVRASGEPRKEQPAVPLGPEEKIKREWQAYAVGA
                     PHGLTPVPLWRAERILICAYVDGVPLKKEAERNGTYLDVAAAALPHIARLHRLGVTHL
                     DMSLSNILAEETGRRVFVDFEYGPAAGLTFEQQCLHDYLRLLESVWKFLDPPQRGRVG
                     RVWDEALRAHAPDQVRAAEIAPLRPALTRILAAPEMRVFWKGL"
     gene            complement(1059..2183)
                     /locus_tag="PLZ74_11115"
     CDS             complement(1059..2183)
                     /locus_tag="PLZ74_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98910.1"
                     /translation="MSDLSNRSLPFRTWLDQNRFRYAVLWNLDAPTAKNPLCPGDRHV
                     IVLEDRAIQPADQHWGCRWRHPRNPYRVYSVNGENGTDYLGHPFLPTALAENLLAHTQ
                     QDASGLPMPAPRDRYDALLYHLAYHLAETSGADAEDPLAFRNSPWHTQVRSAGEPLGL
                     PYDLSLLDMHRWLKEHGYGFSAARRTAYLQHQFKNRFKSAFYAFALCMEMENQGEMNL
                     FVLRSKVIRHGFRQRLLDEIARHYTILAVRDIPWLTRLRKARHMRGNKWRWGGWPVVA
                     VVVFDPAPIWRTRAERAQEHSFVLNSRQFFKRELREQIVQEGGLYHKHNAMHTTDNEA
                     EAIGHLHLFFPPEAMQEVFDRLAAARSALDPSAPPNAFVP"
BASE COUNT          469 a          685 c          853 g          486 t
ORIGIN      
        1 gaaaccctga aaccaaagag agttgaaggt tcaactgctc ttttaatgca tataaggcat
       61 gttctcggaa gaggttgttg aaacgtcacg ttgcgattgt cccgtgtttg caaatccggt
      121 atgatcagaa acagatcggt acggcatgtg taacgatagg ggaatggcgg atgagggttc
      181 tgaaacgatg gtggttgcgg agtcgagcgt ttcgcgcggc gcaaccggaa ctggaagcct
      241 gccgcgcgac catggagcgg ctgttcggcg gtccggtcac atggcggcgc acgggaggat
      301 gcggccgcga tgtcgtttgt ctggtgtcgt gcggggggaa gccggttggg gtgttgcgga
      361 gggtcgcgcc ggttgcggtg cgcgcaagcg gggaaccgcg gaaggagcag cctgccgtgc
      421 cgctcggtcc ggaggaaaag atcaagcgcg agtggcaggc ttacgcggtc ggtgcgccgc
      481 acgggttgac tcccgtaccg ctgtggcgcg cggagcggat cctgatatgt gcgtacgtgg
      541 atggggtgcc gctcaaaaag gaggccgagc gcaatggaac ctatctggat gtggcggcgg
      601 cggctttgcc gcacattgcg cggctgcacc ggttgggtgt tacgcatctg gacatgagcc
      661 tttcgaacat tctggcggaa gaaacgggac ggcgggtgtt cgtggacttt gagtatggcc
      721 ccgcagcggg gctgacgttc gaacagcagt gcctgcatga ctaccttcgg ttgttggaaa
      781 gcgtctggaa gtttctggat cccccgcagc gaggccgtgt cgggcgcgtt tgggacgagg
      841 cgctccgtgc gcatgcgccg gatcaggtcc gcgcggccga gatcgcgccc ttgcggcccg
      901 cattgacgcg tattcttgcc gcgcctgaga tgcgggtttt ctggaagggg ctctgatgtc
      961 gcgggacgag gtgtgcgggc ggatgcgttg cgttcagtcg ggaagggctg gcgacggccc
     1021 cccgttttgg aacgggatcg cctcttcccg cgcgcgggtc acggcacgaa cgcgtttgga
     1081 ggggccgacg gatccagcgc ggagcgcgcc gccgccaatc ggtcgaaaac ctcctgcatc
     1141 gcctccggag gaaaaaacag gtgcagatgg ccgatcgctt ccgcctcatt atccgtggtg
     1201 tgcatggcgt tgtgtttgtg atacagcccg ccttcctgaa cgatctgttc gcgaagttcc
     1261 cgcttgaaga attgccggct gttcagcacg aacgaatgtt cttgagcgcg ttccgcccgc
     1321 gtgcgccaga tcggcgcggg atcaaacacc acaaccgcca caacgggcca gccgccccac
     1381 cgccatttgt tcccccgcat gtggcgggct ttccggagcc gggtgagcca gggaatatca
     1441 cgcacggcca agatcgtgta gtggcgggcg atttcatcca agaggcgttg gcggaaaccg
     1501 tggcgaatga ctttcgaccg caggacaaaa agattcatct ccccctggtt ctccatctcc
     1561 atacacaacg cgaaagcgta aaaagcgctc ttgaagcggt ttttgaactg atgctgcagg
     1621 tatgccgttc gacgggccgc gctaaagccg tagccatgtt ccttcaacca gcggtgcata
     1681 tctaacaacg aaaggtcgta aggcaaaccg agcggttctc ccgcggatcg gacttgggtg
     1741 tgccaggggc tgttacggaa ggcgagcggg tcttccgcgt ccgcaccgct ggtctcggcc
     1801 agatgataag cgagatgata gaggagcgcg tcataccggt cgcggggggc aggcatcggc
     1861 aggcctgagg cgtcctgttg ggtgtgagcc aacaggttct cggcgagggc ggtaggcaga
     1921 aaaggatgcc cgagataatc ggtgccgttt tccccgttca cgctatacac ccgatacggg
     1981 ttccgggggt gccgccatcg gcagccccag tgctggtctg caggctggat cgcgcggtct
     2041 tccaatacga tcacgtgccg gtcccccggg cagagggggt tttttgccgt gggcgcgtcg
     2101 agattccaca ataccgcata acggaaacgg ttctggtcaa gccaggttcg aaaaggaagg
     2161 gatcggttcg aaaggtcact catgctgtca cgtcaggtct gtcgggatat gaattccggc
     2221 aatcatagcc gcatcgatta aggaacgcaa gcctgcgaga cttcggatcc atgatcaaac
     2281 aaacatgaac cgtgatgcgt ttcaccagta agattacacg tccgcatggc ccggcggcgg
     2341 tttgcgggga aaccgccctg ccacgcgggg tagggacgcc cgcttctccc gtcagacctt
     2401 atgtcgaccc gcgacccgcg acgagcgacc tgcggtgccg gaaacgcggg cgtccctgtc
     2461 gatcgtcgcg ggtcgaatgt cgcgggtcgc ggg
//
LOCUS       DAOSRB010000306         2461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_38833_length_2461_cov_5.887365, whole genome shotgun sequence.
ACCESSION   DAOSRB010000306 DAOSRB010000000
VERSION     DAOSRB010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38833_length_2461_cov_5.887365"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..903)
                     /gene="rsmA"
                     /locus_tag="PLZ74_11120"
     CDS             complement(46..903)
                     /gene="rsmA"
                     /locus_tag="PLZ74_11120"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOR98911.1"
                     /translation="MNLTSPSQVKAWCIANDFHPNKTLGQNFLIDRNTLKATLDAAGV
                     SSGCRVLEIGPGLGVLTEGLLSRGARVTAVEKDTRLASRLAEALGDPPELRVLAADAL
                     EVAWDALLAEGFDACVSNLPYSVGTRILLDLAPHPLAPPVFVVLVQSEVAERFAAPPG
                     RAGRSQAGVWLQLDYTVTPVRTVKASCFWPRPAVASALVRLDRQPCALTPTERVWFHD
                     LTRYAFMHRRKQLGGLLRKAPAPLGREEGALAVLFHSAGVDPLARAEQLTNTQWQSLA
                     RAFAEGVRG"
     gene            complement(908..1870)
                     /locus_tag="PLZ74_11125"
     CDS             complement(908..1870)
                     /locus_tag="PLZ74_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOR98912.1"
                     /translation="MVCAALCGLADLTTEKKRSAVLLDGVAAYVNTEMITIADVMGEV
                     RRSPWADTAPELREKRLRELYTATLNALIDRKLILAAARKSKMELQSWAVDNRVREIV
                     ANNFDGDQTKLHALLSDRKITFEEWKKNIEEDLLITAMRYQQVEKRVTPTPTEIRAEY
                     EANKGRYQTETAMAVSMIILDPPEKEGDESVAVRAEKIEAALKAGTSFASLARTYSKD
                     AKAADGGSWGKVNPVDVFRKEIVDALDKLKPGETAPVVVLDGYGYIVRKDEQQDARAL
                     TFEEAAPYVESHLRMRQAEKLYKEWTDRLRAESYIKVFELPTSK"
     gene            complement(1755..>2461)
                     /locus_tag="PLZ74_11130"
     CDS             complement(1755..>2461)
                     /locus_tag="PLZ74_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98913.1"
                     /translation="AVIGADRFNYSYDPIGNRVWSSMNTLTNTYAANRLNQYTLISNP
                     VNPVNPVLISAYDYANDAAGRRVSRNDDAFGYNARSEVISAAIGTNGYAYAYDPIGNR
                     VWSAMNAVTNTYTANRLNQYTLISNPVNPVNPVQILPTYDADGNMRWDGSEGYVGFTV
                     AFRPALGYTIFRLRGRRVAGRRRRKTNEAYRMDCRRSGLRGPLRVGRPDHGEKAFGRP
                     VGRGGRLCEHGDDHDR"
BASE COUNT          461 a          790 c          747 g          463 t
ORIGIN      
        1 agaacacaac ccctcacccc ctcacagcct aaccccctaa ccccctcacc ccctaacccc
       61 ctctgcgaac gcgcgggcga ggctttgcca ctgggtgttg gtgagctgtt cggcacgggc
      121 gagcggatcc acgccagcgc tgtggaacaa cacggcgagt gcgccttcct ctcgaccaag
      181 cggcgcgggc gccttgcgca gcaggccccc caactgttta cggcggtgca taaaggcata
      241 ccgcgtgagg tcgtggaacc agacacgttc cgttggagtg agcgcgcagg gttgacggtc
      301 aagccgcaca agggcggaag cgaccgctgg ccgcggccag aaacaggaag ccttgaccgt
      361 gcgcaccggt gtgaccgtgt aatcaagctg gagccagacg cccgcctgac tgcgcccagc
      421 cctgcccggc ggcgcggcga aacgctcggc cacctcggac tgcaccagca ccacaaaaac
      481 cggcggcgcc aacggatgcg gggccagatc gagaagaatg cgtgtgccca ctgaataagg
      541 caggttggag acgcatgcgt cgaacccctc cgccagcagc gcgtcccacg ccacttccag
      601 cgcgtcggcc gccagaacgc gcaattccgg cggatcgcca agcgcttcgg ccagccgcga
      661 ggccagccgc gtgtccttct ccaccgccgt gacccgcgcc ccgcgagaga gcagcccctc
      721 ggtgagcacc cccagccccg gaccgatctc cagcacgcgg caaccggatg acacgcccgc
      781 cgcatccaag gtggccttaa gggtgttgcg gtcgatcaga aaattctgtc ccagcgtttt
      841 gttgggatgg aagtcgtttg cgatgcacca ggctttgacc tgcgatggcg acgtgaggtt
      901 cacaacatca cttggaggtc ggcaactcaa acactttgat gtacgactcc gcgcgcagcc
      961 gatccgtcca ctctttatag agcttctccg cctgccgcat gcgcaggtgg ctctcgacgt
     1021 acggcgccgc ctcctcaaaa gtcagcgcgc gcgcgtcttg ctgctcatcc ttgcgaacga
     1081 tgtaaccgta tccatccagc accaccaccg gcgcggtctc gccgggcttg agtttgtcca
     1141 gcgcatcgac gatctccttg cggaagacat ccacagggtt caccttgccc cacgaaccgc
     1201 cgtcggccgc cttggcgtcc ttggagtagg tgcgggccag cgaggcaaag gaggtgccgg
     1261 ctttgagcgc ggcttcgatt ttctcagcgc ggacagccac actctcatcc ccctcctttt
     1321 caggcgggtc gagaatgatc atgctcacgg ccatcgcggt ttcggtctgg taacgccctt
     1381 tgttggcttc atattcggcc cggatctcgg tcggagtcgg agtcacgcgc ttctcgacct
     1441 gctgataccg catggcggtg atcagcagat cctcctcgat gttcttcttc cactcctcga
     1501 aggtgatctt gcggtccgag agcagggcat gcagtttggt ctggtcaccg tcaaaattgt
     1561 tggccacgat ctcgcggacg cggttgtcca cggcccacga ctgcaactcc atcttggatt
     1621 tccgggccgc agcgaggatc agcttacggt cgatcagcgc gttgagcgtc gcggtgtaca
     1681 gttcgcgcaa ccgcttttcg cgcaattccg gcgcggtgtc ggcccacggg ctgcgccgca
     1741 cctcccccat cacatcagcg atcgtgatca tctccgtgtt cacataggcg gccaccccgt
     1801 ccaacaggac ggccgaacgc tttttctccg tggtcaggtc tgccaacccg caaagggccg
     1861 cgcaaaccac tacgcctgca atccatccga tacgcttcat tcgtttttct ccttcgtcgc
     1921 cccgcgacac gacggccgcg taatcggaag atagtgtaac ctagcgccgg acggaatgcc
     1981 acggtaaaac caacgtatcc ttcagaaccg tcccaacgca tgttgccgtc ggcgtcgtag
     2041 gtggggagaa tttggacagg attgacagga ttaacaggat tggagatcag ggtgtactgg
     2101 ttgaggcggt tggcggtgta ggtgttggtg acggcgttca tagccgacca gacgcggttg
     2161 ccgatggggt cgtaggcgta ggcgtaaccg ttcgtgccga tggccgcgct gatgacctcg
     2221 gaacgcgcgt tgtagccgaa ggcgtcatcg ttgcgcgaga cgcgccgccc ggcggcgtcg
     2281 ttggcgtagt cgtaggcaga gatcaagaca ggattaacag gattaacagg attggagatc
     2341 agggtgtact ggttgaggcg gttggccgcg taggtgttgg tcagggtgtt catagacgac
     2401 cagacacggt tgccgatggg gtcgtacgaa taattgaaac ggtcggcacc gatcaccgcg
     2461 c
//
LOCUS       DAOSRB010000307         2422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_39679_length_2422_cov_9.222645, whole genome shotgun sequence.
ACCESSION   DAOSRB010000307 DAOSRB010000000
VERSION     DAOSRB010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39679_length_2422_cov_9.222645"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLZ74_11135"
     CDS             complement(<1..584)
                     /locus_tag="PLZ74_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98914.1"
                     /translation="MSYKASRRDFLKLSAVAGYGTAAAQVLKPTSVRAQGSAGTTVMG
                     LRTPKMERVRVGFIGLGARGGGHLGDILKIEGAVVTALCDTHQPTLDRATKRVTDAGQ
                     PQPAVFGDGPDAYKKMLARDDVDIVYIATPWELHTPQALAAMQAGKHAFIEVPAAITT
                     EECWALVDTAEKTQRHCMMLENVNYGREELMVLN"
     gene            770..2167
                     /locus_tag="PLZ74_11140"
     CDS             770..2167
                     /locus_tag="PLZ74_11140"
                     /inference="COORDINATES: protein motif:HMM:NF016345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HOR98915.1"
                     /translation="MTALLTNPVHAVRCLGIVMLVLHAASLDARAQARVQCGYEPLPS
                     GFTFEHVPLPANNDAATAARFSLVDGQPDRNGAPLAALHDGAVPLNEDDPARNFFFQA
                     GSDGGRLVIDMTRNLSIAYIATFSWHRGSRGPQVYTLYAANGLPSIFQREPRRGTDPT
                     SCGWIRLARVDTRPKDGSEGGGQHGVAIRAPSGRLSTFRYLLFDVEPTQQHDPFGHTF
                     FSEIDVVEAGGPPVTSMLRPLKRVRRTFTLPDASCTFLLDATDAPDLVPWAEKTLRPA
                     LTAWRPKITALLSSADYTPPARVTLLFCNDMNAPAAASGGIIRLNAAWFRREIEGEAT
                     GCVIHELVHVIQNYGRPTRNAQPPAPVPGWVVEGIADYIRWFLFEPERRGAEITSRRA
                     IEAARYDGSYRVSANFLNWAVTKYDRSLITRLNAAAREGRYDDALWQTWTGKHLPELD
                     KEWKHDLIRRLPPTH"
     gene            complement(2243..>2422)
                     /locus_tag="PLZ74_11145"
     CDS             complement(2243..>2422)
                     /locus_tag="PLZ74_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98916.1"
                     /translation="NRWVTNELQFVTFDDPGFANMQQRDFRLKPDSPVFTRIPGFKPL
                     PLEKMGLRRPARCAD"
BASE COUNT          485 a          808 c          649 g          480 t
ORIGIN      
        1 atgttcagca ccatcagctc ctcgcgccca tagttgacgt tctcaagcat catacagtga
       61 cgctgcgtct tctccgccgt atccaccagc gcccagcact cttcggtcgt gatcgcggcg
      121 ggaacctcga taaaggcgtg cttgccggcc tgcatcgcgg ccagcgcctg cggcgtgtgc
      181 agttcccacg gggtcgcgat atagacgatg tcaacatcat cccgcgcgag catctttttg
      241 taggcatcgg gaccgtcacc gaacaccgcc ggttgcggct gtcccgcatc ggtcacacgc
      301 ttggtcgctc tatcaagcgt aggctgatgg gtgtcgcaca gggccgtgac cacagctccc
      361 tctatcttca ggatgtcccc cagatggccg ccaccgcgcg cccctaggcc gataaagccc
      421 acgcgcacac gctccatctt cggcgttcta agccccatca cagtagttcc tgcactacct
      481 tgggcgcgta ccgaagtagg cttcagtacc tgtgcggcgg ccgtaccgta tccggccacg
      541 gctgacaatt tcagaaaatc acgacgcgat gctttgtacg acatttcttt ctccctgctc
      601 ttttgtctgt tgcacagttt caattgaaaa ctgtgtgata tattaagtct tagcgtgccc
      661 atcaggcaag cgtaaaggac gtgaatgaac ttcctcgcgg ggatagacag ccgcccccga
      721 ttgtgcgaca ctgcctcgta gctaccacat gatcgagacc tatcttacga tgacggcact
      781 acttactaac cccgtgcatg ctgttcgatg cctcggcatc gtcatgctcg tactgcacgc
      841 cgcgagcctc gatgcgcgcg cacaggcccg cgtgcaatgc ggctacgagc cgttgccatc
      901 agggttcacg ttcgagcacg tcccgttgcc tgccaataac gacgcggcaa cagcggcccg
      961 attctccctt gtggacggcc agcccgaccg caacggcgcg cccttggctg cgctacacga
     1021 tggcgccgtt ccgctgaatg aagacgaccc cgcccgtaac ttctttttcc aagccggcag
     1081 tgacggcggg cgtctcgtca tcgacatgac ccgcaactta tccatcgcat acatcgccac
     1141 tttttcatgg catcgcggat cgcgcggccc ccaagtttat acgctctatg ccgccaatgg
     1201 cctgccatcg atcttccagc gcgaaccccg gcgcggtacc gatccgacct catgcggctg
     1261 gatacgtctc gctcgtgtag acacacggcc gaaagacgga tcggagggcg gcgggcaaca
     1321 cggcgttgcg atccgtgcgc cgtccggtcg gctgagcacc ttccgctact tgcttttcga
     1381 tgtcgagccc acccagcagc atgacccatt cggacacacc tttttcagcg agatcgatgt
     1441 cgtcgaagcg ggaggaccac ccgtaacatc catgctccgt cccctaaagc gggtgcgccg
     1501 cacctttacc ctgcccgatg cgagttgcac ctttttgctg gacgcaacag acgcgccgga
     1561 tctggtgccg tgggccgaaa agacgctccg cccggcgcta accgcttgga gacctaagat
     1621 taccgcattg ctatcctctg ccgactacac gccgcccgcg cgtgtcacgc tgctgttttg
     1681 caacgacatg aacgcgcccg ccgctgcgag cggcgggatc atccgcctca acgcggcatg
     1741 gttccgccgc gaaattgagg gggaagccac cggctgcgta atccatgaac tggtacatgt
     1801 gatacagaac tacggtcgtc caacgcgcaa tgcacagccg cctgcccccg tgcctggctg
     1861 ggtggtggaa ggcatcgccg actacatccg ctggttcctt ttcgaaccgg aacgccgggg
     1921 ggcggaaatc acttcgcgcc gagctataga ggcagcccgt tacgacggca gctatcgcgt
     1981 cagcgccaac ttcctgaact gggcggtcac gaagtatgac cggtcgctca tcacgcggct
     2041 gaatgcagcc gcccgcgaag gacgttatga cgacgccctc tggcaaacct ggaccggcaa
     2101 acatctccca gaactagaca aagagtggaa acacgacctg atccgtcgcc taccgccaac
     2161 gcattgagcg atcttgcaga acggcaaaac tcctaactcc taaatcctaa atcctaaatc
     2221 ctaaatccca actcctaaat catcagtccg cgcaacgcgc gggacgccgt agccccatct
     2281 tctcaagcgg cagcggcttg aaccccggga tgcgcgtgaa gaccggcgaa tcgggcttca
     2341 accggaaatc ccgctgctgc atgttggcga acccgggatc gtcaaatgtc acgaattgga
     2401 gttcgttggt cacccagcgg tt
//
LOCUS       DAOSRB010000308         2416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_39838_length_2416_cov_5.094452, whole genome shotgun sequence.
ACCESSION   DAOSRB010000308 DAOSRB010000000
VERSION     DAOSRB010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39838_length_2416_cov_5.094452"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1960
                     /locus_tag="PLZ74_11150"
                     /pseudo
     CDS             118..1960
                     /locus_tag="PLZ74_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611810.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
BASE COUNT          565 a          695 c          671 g          485 t
ORIGIN      
        1 gcgtgattga gatcaaacgg gatagtcgtt ctcattaaga aacacgaaac caccgacgtg
       61 cttcgccccc cgtgaacggt tacgattttt ttgatacaat aatttaaaaa aaccccaatg
      121 tttttgaggg gtgaatgtcc aaaagtcgtt gtagtacaga ccttgcccct tacggttgcg
      181 ctacgctttt gcgataatgc gtggcatgta cctcaaatgt cacaagagat tcaaggatgg
      241 gaaagagcat cgttactgga gcatcgctga aagcataaga acgcgtaacg gagtcagtac
      301 gcgtcaagtc ctctatctgg gcgagatcaa tgactcccag cagcgtcagt ggtgttctgc
      361 aatcgaggtg gttgacggcg gggatgcagg cgtccgccag atgagcctgt ttcccgagga
      421 tcgcgtcccg cccgccgggg ttgtgaaccc cgtgcgcgtc cgtctcgacg cgatgaccct
      481 gcaccgcccg cgtcaatggg gcggctgttg gctgtcgctg gagctctgga ggttgttgga
      541 tatggagtcg ttctggggca tgcgtttggc tgatagcaga aaggggacgc gttggacgga
      601 cgtattggca tcgctcgtgg cctatcggct catcgacccg gggagcgaat ggcggatgca
      661 ccgcgaatgg ttccgcaaca cggcgatggc cgatctcctt gggtgcgacg agagcgtggc
      721 cgcgaagaac acgctctacc gctgcctcgc caagctttgc aaacataggg atgatctctt
      781 caagcacctc cggggacgct ggggggcatt gttcggcgcg aaatgcgaca tcctgctgta
      841 cgatctgaca agcacctact ttgaatgcga tgttccaggt gagttcggac tccgccgttt
      901 tggatacagt cgcgacaggc ggcccgattg cgttcaggtc gtcatcgcgc ttgtcgtgac
      961 ccccgatggt ctcccagtcg cctatgagac gatgcaggga tgtacgtcgg acaagaccac
     1021 gctgaaggac tttctggcaa aaatcgaaac gatgtatggc aaagctggcc ggacatggat
     1081 catggatcgc ggcatcccca ccgaggaggt gctcggcgaa atgcgagatg cgaagacaag
     1141 ctatctcgtg ggcacaccgc gcggacggct cacaaaaatc cagcgcgagg tcgccgctca
     1201 gccctggcgg aaggctcggg acggcatcca agtgaagacc tttgcaggcg aggacggcga
     1261 cacctatgtc ctgacccgca gttgcggccg ccgcgacaaa gagcaggcca tgcgccgcag
     1321 aaagctcaag ctgttgatcc ggcggctcgg agagatcaca cggatgaagg atctttcccg
     1381 cgacgcactg gtggcgaagg tcgccgtggc taggcacgag gcggggcgct ccgcagcccg
     1441 catcatggat atcagtctgc cgacagacgg cgcgccatta tccttcaagc tggatcgcgc
     1501 aaagctacgc gtcgttcgga ggggcgaggg tacgtatctg ttgcggacca acctgccgcc
     1561 cgagaacccg gaagacctct ggcggctcta catcacgctc acggaggtcg agcaggcctt
     1621 caaggacctc aagaacgatc tctccatccg ccccgtcttc caccagaccg acgcgcagat
     1681 cgaagcacac atcttcgccg ggttcctcgc ttactgcctt ttcgtgactc tcaagcagat
     1741 atccaaggga ctcgcgccgg ggctcacgcc acgcgccgtc ctggacaagt tcaaaaccgt
     1801 gcagatgctc gacgtccact tccccacgac agacggccac accctcgtgc tgccgcgtta
     1861 tacacaacca gacaaggatc tccagatact ccttcaccga ctcgatatgc gactcccgga
     1921 gcagcccccg ccacgcatcg aagccccatc cgggaaatga aatgtagggg aaacctttga
     1981 ctaaaacccc agcaatatcg atagtttttg cctcgaacgg ggaaagtcgg gctaaaattg
     2041 tgcaaggcag ctatgatgac aacctgtgct ccgaaatcac ttccatcagt cgtcccctca
     2101 ccagcatttc tgcgcaagaa acacgtggct tcgcttatga ctatgattac gatacatacc
     2161 aaacacgtcc caaaagaaaa taaaattcgg catcttgaca agattatcaa attttggtaa
     2221 aagtgaacgc gaatagcaga agagttcttg ttgtatgctt tgcttggaga gttaaaatga
     2281 attacgttct tatccccaag tccgctaccg ggcggatcgc gctctggcta ctgccgccca
     2341 cgccacaggc acaaacattc tccccctgcc aaacaaaatg aacagccaat ttccaacctc
     2401 gacaatcacc atggct
//
LOCUS       DAOSRB010000309         2367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_40966_length_2367_cov_3.730536, whole genome shotgun sequence.
ACCESSION   DAOSRB010000309 DAOSRB010000000
VERSION     DAOSRB010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40966_length_2367_cov_3.730536"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            606..1967
                     /locus_tag="PLZ74_11155"
     CDS             606..1967
                     /locus_tag="PLZ74_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOR98917.1"
                     /translation="MASTGSGSIGFAASPLLKDETAGEIRVEDGTLTLTAPFCWLLNG
                     IVKSGSGHLRLTTKNDYTGNTRIDGGTLILANDATLSSSTVVFNSADAALMLESDASV
                     GGLAAQGASKSGSAAFDGLKSAAEYNNLCMANGLWIGGVLLVGTRDSVILRQSWGWTS
                     TAKNMPMRADAIFDLASVTKAVGTTTALALCIDRGWLDPDVVFTNYLPGYKGTLRGPV
                     TVRDLARHLSGFNNSKPYAVDGRVTELILEFSPVRPAGEPYEYSCGNFILLGLLVEQL
                     AGKSLAEFCQDNVWAPLAMQDTRWTPLPTPDPRRVVKPPFTPTLGVVSDEPARAAGRP
                     IGNAGLFSTADDLAAYCRMLLRGGVCNGTRILSESSVQMFGTRPDTRSPAAFGWRVSA
                     GLNPSALSAATLSHTGFTGQSVWIDPAQGRFVILLTNRIGDHAAAGQARTELADRVLR
                     ALQ"
     gene            2044..>2367
                     /locus_tag="PLZ74_11160"
     CDS             2044..>2367
                     /locus_tag="PLZ74_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98918.1"
                     /translation="MRHNTGTLRGWTCVILSCAVCAAQATTGIWTNVSSGYWADSANW
                     QGGVVPSTANTMPGTGDVADFTALADGETVTITNHTACGALIFSNTVGTSWTLLGSTM
                     KLANSP"
BASE COUNT          524 a          623 c          720 g          500 t
ORIGIN      
        1 gctcctgaaa ctcaaagcgc aacaggaacg ccgtcaggta cgccagcgcc atcactgccg
       61 tatccgccgc atggacgcct gccctgacag cccagacgcc caaccgctct ttccaatttc
      121 caaccatgac gtgcctatta tttaagcaga tcagcccctc tgagaacagt cttgattgtg
      181 aaacaaaaaa acgctgacac ggccagtagg cgaggattcg cccaatagaa gtcattctgc
      241 gcgaaacggg gtggtttgct actagagaaa atggtaaagt ggtgacgctg gcttgggcgg
      301 gtgccttttc aggtagcaac aagtggagat cgaatgtgaa aacacacgtt agacgggtac
      361 tatggtgggg gtgcgcggcc atctccgtat gtgcggtctc ggcacgagcg gtgacgggcg
      421 tctggacaaa tgtctcgggg ggctactggg ccgatagcgc gaactggcag gatggtatag
      481 tgccttccgg tgtcggggct gtcgcagact tcagcgcgtt gcactcggga tcgacggtta
      541 cgataacgaa ttacacaggt atcggtgcga ttgtattttc cggtgtatca ggggacaact
      601 ggaccttggc atcgacggga tcaggatcaa ttgggttcgc cgcttcaccg ttgcttaaag
      661 atgagacggc tggggaaatc cgggttgaag atggcacact gaccttgacc gctcctttct
      721 gttggctgtt gaacggaatt gtgaagagcg gatcggggca tctgcgatta acgaccaaga
      781 acgattacac aggcaacaca cgtattgatg gagggacgct gatccttgca aacgatgcca
      841 ctctgagcag cagcacagtg gtgtttaact cggcggacgc cgcactgatg ctggaatcgg
      901 atgcaagcgt cggcggtctc gcggcacaag gcgcatcgaa aagtggatct gccgcttttg
      961 acggactgaa aagcgctgcg gaatataata atctgtgcat ggcgaacggc ctgtggatag
     1021 gcggtgtttt attggtaggc acccgcgact cggtgatctt gcgacaatct tggggatgga
     1081 cctcgacggc aaaaaacatg ccgatgcggg cggacgccat ttttgatctc gccagtgtga
     1141 ctaaggcagt cggtacgact acagccttag ccctctgcat tgatcgcggg tggctcgatc
     1201 cggatgtggt cttcaccaat tacctgcccg gctacaaagg aacgctacgc ggtcccgtca
     1261 cggtccgtga cctcgcccgc cacctttccg gttttaataa cagcaaaccg tatgcggttg
     1321 atgggcgggt gacggaactg attcttgaat tcagtccggt ccgtccagcg ggcgagccat
     1381 acgagtattc ttgcggcaat tttatcttgc tggggcttct tgttgaacag ttggccggaa
     1441 aatcgctggc agaattttgt caggacaatg tttgggcgcc gcttgccatg caggacacgc
     1501 ggtggacacc gttgccgaca cctgacccgc gccgcgttgt gaagccgccc ttcactccga
     1561 cattgggtgt cgtcagtgac gagccagctc gggccgccgg acggcctatc ggcaacgcag
     1621 gcctcttctc gacggcggac gatctcgccg catattgccg aatgttgcta aggggcggtg
     1681 tttgcaatgg cacacggatt ttgtctgagt cttcagtgca gatgttcggc acacgaccgg
     1741 acacgcgctc gccggcagcc ttcggctggc gtgtaagcgc gggcctaaac ccctcggcac
     1801 tgtcagccgc gacgctgagc catacgggat ttacaggcca atccgtctgg atcgatccgg
     1861 cccaaggacg gtttgttatt cttttgacaa atcggatcgg cgatcatgcc gccgcaggac
     1921 aagccagaac cgaattggct gaccgagtat tgcgggcact gcaatgattg ccgaggccga
     1981 caaagtggac acaaaacaaa acacggacag caaacgattc ctcaaggtga aggaggcatg
     2041 ccaatgagac acaacacggg tacgttgcgc ggttggacgt gcgtgatttt gtcgtgcgcg
     2101 gtgtgtgcag cacaggcgac gaccggcatc tggacaaatg tttcgagcgg ttactgggcc
     2161 gacagcgcaa actggcaggg cggcgtggtg ccgtctactg cgaatacaat gccgggcacc
     2221 ggggatgttg cggattttac cgcactggcg gatggagaga cggtgaccat aaccaaccat
     2281 acggcatgcg gggctcttat tttctcaaat accgtcggaa cctcgtggac attgctgggc
     2341 agcacaatga aactggcaaa ctccccg
//
LOCUS       DAOSRB010000310         2356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_41251_length_2356_cov_6.335941, whole genome shotgun sequence.
ACCESSION   DAOSRB010000310 DAOSRB010000000
VERSION     DAOSRB010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41251_length_2356_cov_6.335941"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..424
                     /locus_tag="PLZ74_11165"
     CDS             8..424
                     /locus_tag="PLZ74_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98919.1"
                     /translation="MAECIYYRKGYKYQLAEDDVSYTAIRPVADIVTDFIELTKEGRL
                     LIRKAYAWDGCSGCAIDDKTNMRGGLRHDAKYQLMRLGLLPPEDREIADIELREDCLD
                     AGMWKIRAWYYFEGVDHFAEFAARYGYEPYPILIAP"
     gene            439..648
                     /locus_tag="PLZ74_11170"
     CDS             439..648
                     /locus_tag="PLZ74_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98920.1"
                     /translation="MKNWKTWLYGLASAAIGAAANTVTVMIVAPETFNLGEGLNKVLM
                     AAGSSAIVAVALYLKQSPLPGGAEK"
     gene            760..1494
                     /locus_tag="PLZ74_11175"
     CDS             760..1494
                     /locus_tag="PLZ74_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98921.1"
                     /translation="MKKLLIALILLLAVPVWAGPHIEYVTPAASETVAGTTERCTDAE
                     MVAGTSTACAGTPANVKAKLGPQTDGGILIGAGQAAAIEATAAGATTEILVGGGAGAP
                     PVWTTATGSGAPVRATSPSLTTPNMTVSEYVEAASDTLSALQVSGGQINNYGQADDTT
                     IILPACAAGYNFDVILGTTVAKYYRLDPAAGDLIILDGVAGTDGKYIGIASAVAGAAV
                     TCRAIQTGASAYDWACYTVSGAWVAE"
     gene            1491..2315
                     /locus_tag="PLZ74_11180"
     CDS             1491..2315
                     /locus_tag="PLZ74_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98922.1"
                     /translation="MMNRYRFAFAILAICATVTLLSVPVITTTEARTILPAKVVLFKS
                     TTEAPRISAVATGAFLDNLSAGAMAVIAAYPLLQVDVYYGQTLLRAYTGSAAIGDGED
                     LTEEIAAGDDRTFASDTGWWSKSVAQVTIADGVCHFAAAANGSMLYRSAGNIAPTCLY
                     KTIFTVANRTGGAAYVRLGGVGGATGSHRTTNDTFTQYLTVSATPTNTSLGFGCYDQS
                     TFDIDNVSVSQVTGPSTSGCLLYNSRALSTQSVAVNTFAKGSYNQSAYTLILSKPR"
BASE COUNT          513 a          638 c          728 g          477 t
ORIGIN      
        1 gtgaggcatg gctgagtgca tctactaccg caagggctac aagtaccaac tggcggaaga
       61 cgacgtttct tataccgcca ttcggcccgt tgcggacatc gtaaccgatt ttatcgagct
      121 gacaaaggaa ggccggcttc tcatcagaaa ggcctacgct tgggacggat gcagcggctg
      181 tgcgatcgat gacaaaacga acatgcgggg cggactccgg cacgacgcaa aatatcaact
      241 catgcggttg ggccttctgc ccccggagga ccgagagatt gccgacatcg aactgcgcga
      301 ggattgcctt gatgccggca tgtggaagat ccgggcgtgg tactacttcg agggcgtcga
      361 ccatttcgcg gaatttgcgg cgcgttatgg atacgaacca taccccattt tgattgctcc
      421 atagcaggag gacgggaaat gaagaactgg aagacctggc tttacgggct ggcgagcgcg
      481 gcgattggtg cggcggcgaa tactgtcacc gtgatgatcg tggcgcccga aaccttcaat
      541 ctcggcgaag ggctgaataa agtcttaatg gcggcgggat cttcggcaat cgtggccgtg
      601 gcgctgtacc taaagcagag tccgcttcct ggcggggccg aaaaatgaaa cgcatgtccg
      661 aacttgaggc aatagagcgg atcttctgga cggtcctctt tgtcacctct tgatttggca
      721 gacggtcaaa ctctacgaac tcttgaaata gggggcacta tgaaaaagct gttgattgcg
      781 ctgatcctgt tgctggcggt cccggtatgg gccggtccgc acatcgaata tgtgacgcct
      841 gcggcctctg agaccgtggc gggtacgacg gagcgatgca cggatgcgga gatggtggcc
      901 gggacgtcta ccgcgtgcgc ggggacgccc gcaaacgtaa aggccaagct cggacctcag
      961 acggacggcg gtatcctgat cggggccggt caagccgccg caatcgaggc gacggctgcc
     1021 ggggcgacga cggaaatctt ggtaggcggc ggggcgggtg cgcctccggt ctggacgacg
     1081 gcgacgggat cgggtgcgcc ggtaagggcg acgagcccgt ccctgacaac gcccaacatg
     1141 accgtttccg aatatgtcga agccgcgagc gacacattga gcgccctgca agtctcgggc
     1201 gggcagatca acaattacgg acaggcggac gatacgacca taatccttcc ggcctgcgct
     1261 gccgggtaca attttgacgt gatcctcggc acgaccgtag ccaaatatta ccgcctggac
     1321 cctgccgctg gcgacctcat cattttggac ggcgtggccg ggactgacgg caaatatatc
     1381 ggcattgcct ctgcagtagc gggtgcagcg gtgacttgtc gggcgataca gaccggggcg
     1441 agtgcctacg attgggcctg ctacacggtt tcgggggcat gggtggccga atgatgaacc
     1501 gataccgatt cgctttcgca atactggcaa tttgcgcgac tgtgacactg ctttccgtgc
     1561 cggttatcac gacgacggag gcgcgaacga ttctcccggc aaaagtcgtg ctgttcaaat
     1621 ctaccaccga agccccccgc atctcagccg tagcgacagg agcctttctg gacaacctca
     1681 gtgccggagc catggccgtc atcgctgcct atcccttgct gcaagtcgat gtgtattacg
     1741 gccagaccct gctccgtgcc tacactggtt cggcggctat tggggatggg gaggatttga
     1801 ctgaagaaat cgcagccggg gatgatcgta cttttgcgtc cgacacgggg tggtggagta
     1861 aaagtgttgc acaggtaacg attgccgatg gcgtatgtca ctttgccgcc gcagccaatg
     1921 ggtcgatgct gtatcgatcc gccggaaata ttgccccgac atgtctatat aaaaccatct
     1981 tcacggttgc aaatcgcacc ggcggggcgg cgtatgtacg gcttggtgga gttggtggag
     2041 caaccggatc gcaccggaca acaaatgata cgtttaccca atatttgacg gtgtccgcaa
     2101 cacctacaaa cacatctctc ggctttggat gctacgacca gtcaacgttt gatatcgata
     2161 acgtgtcagt ttcacaagtc accggcccct cgacctccgg ctgtctcctt tacaacagtc
     2221 gcgccctgtc aacgcagtcg gtggcggtca atacgtttgc gaaggggagc tataaccaga
     2281 gcgcgtacac tctgatattg agcaagccga gatagggcgt agtcaacggt aggcaattcc
     2341 agaaaaaagg ggctac
//
LOCUS       DAOSRB010000311         2355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_41277_length_2355_cov_15.273043, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000311 DAOSRB010000000
VERSION     DAOSRB010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41277_length_2355_cov_15.273043"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLZ74_11185"
     CDS             complement(<1..406)
                     /locus_tag="PLZ74_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOR98923.1"
                     /translation="MLRRDFVSMGLASAALAGCATKMPKKVSTAVAKADGGGDKRMRG
                     PFPILSTPYHEDGSVDYESLKRGVRFTADGECPGVIWCQSNDAVDLLTFEEKVKGYEA
                     CAEAMQGVNCMMTFGCNGVDTAQMLRETEAVEQ"
     gene            complement(421..1863)
                     /locus_tag="PLZ74_11190"
     CDS             complement(421..1863)
                     /locus_tag="PLZ74_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR98924.1"
                     /translation="MLRRDFLKMAAGSAAIPLFSIGGQVAGRKKIRLGLIGCGGRMGL
                     STHYGILNNMCGPDEEIVCMAEPDPNRWDRVRAVVRHHQPTTDTSKIRAFYDYREMLD
                     KMGNAIDAVAIATPNHHHALPALLAMAKGLHVFVEKPMALTIEEVRLMHAAQKKSGVA
                     TQVGNHGHSDEGMRRLVEYIQSGAIGQVREVWAFNDRLNAMMYNPPKADPPKGMDWDA
                     WCGPAPVRDYYAPTEDHNGIHPRDWHAWIGYGNGAIGNMGTHILDPVFWALRLGEVHP
                     TSVEATDLKWGVEGSWTWRNTLHWQFPARKGMDPLTLHWYDGVKDGIPYKKTHVNKTG
                     ICLKRDYQSLPPIIEELEKKYGQNLGCLGTVYIGEKGIMAIGPHGNGLQFVPDTLRTQ
                     LPSPPKTIPREKGMNHTEDWLRAIRNPNRPAGCNFDYSAPLATTVLLGNVAARAGLKK
                     LEWDGTKVTNDESANAFLKTTYRKGWEIKG"
BASE COUNT          435 a          750 c          637 g          533 t
ORIGIN      
        1 tctgctcgac ggcctcggtc tcgcggagca tctgcgccgt gtccacgccg ttgcagccga
       61 acgtcatcat gcagttcacg ccctgcatcg cctcggcaca cgcctcatac cctttcactt
      121 tttcttcgaa ggtcagcagg tcgaccgcgt cgttcgactg acaccaaatc acacccggac
      181 actcgccgtc cgccgtgaag cgcacaccgc gtttgagcga ctcgtagtcc acgctgccgt
      241 cctcgtggta cggcgtggag agaatcggga acgggccgcg catccgttta tcaccgccgc
      301 cgtccgcctt ggcaacggca gtggacacct tcttcggcat cttcgtcgca catcccgcga
      361 gcgccgcgct cgccaacccc attgacacaa aatcccttct cagcatagtt ttccctttct
      421 ttaccccttg atctcccagc ccttgcggta cgtcgttttg agaaatgcgt tcgcgctctc
      481 gtcgttcgtg accttcgtac catcccactc gagcttcttg agtcccgcac gagccgccac
      541 gttgccgagg agcaccgtcg tcgcaagcgg tgcagagtaa tcgaagttac agcctgccgg
      601 gcggttcggg ttgcggatcg cacgcaacca atcctccgtg tggttcatgc ctttctcgcg
      661 cgggatcgtc ttcggcgggc tcggaagctg cgttctgagc gtatccggga cgaactggag
      721 gccgttgccg tgcgggccga tcgccatgat gcccttctcg ccgatgtaca ccgtgccaag
      781 gcaacccaag ttttgcccgt acttcttctc gagttcctcg ataatcggcg gcaggctctg
      841 atagtcgcgt ttcagacaga taccggtctt gtttacatgc gtctttttgt acgggatgcc
      901 atctttgacg ccgtcatacc aatgcagcgt cagcggatcc atgcccttgc gcgccgggaa
      961 ttgccagtgg agcgtgttgc gccacgtcca actcccttcc accccccact tgagatcggt
     1021 agcctccacg ctcgtgggat gcacctcgcc gaggcgcaac gcccagaata cgggatcgag
     1081 gatatgcgtt cccatgttgc cgatcgcgcc gttgccgtag ccgatccatg cgtgccagtc
     1141 gcgcggatgg atgccgttgt gatcctcggt cggcgcgtaa taatcgcgca ccggcgcggg
     1201 cccgcaccat gcgtcccaat ccatgccctt cggcggatcg gccttcggcg gattgtacat
     1261 catcgcgttc aggcggtcat tgaaggccca cacctcacgg acctgaccga tcgcgccgct
     1321 ctggatatac tccacaagac ggcgcatgcc ttcatcgctg tggccatggt tgccgacctg
     1381 cgtcgcgaca ccggattttt tctgcgccgc gtgcatgagg cgtacttctt caatcgtcaa
     1441 cgccatcggc ttctcgacga agacgtgcag gcccttcgcc atcgcaagga gggcggggag
     1501 ggcgtgatgg tgattgggcg tcgcgatcgc gacagcgtcg atcgcgttcc ccatcttatc
     1561 cagcatctcg cggtaatcgt aaaaggcccg gatcttcgaa gtgtccgtcg tcggctgatg
     1621 gtgcctcacc acggcgcgga ccctgtccca acggttcggg tccggctcgg ccatgcagac
     1681 gatctcctcg tccgggccgc acatgttgtt caaaatcccg taatgagtac tgagccccat
     1741 gcggccgccg catccgatca ggcccaaccg tatttttttc cggccggcaa cctgcccccc
     1801 gatggaaaac agcggaatcg ccgcactccc tgcggccatt ttcaaaaaat cacgacgcag
     1861 catctgcacc ccccgtaaaa caggttttta gaaatcccct gcgccacccc gaaaagacac
     1921 aggcaccccg aatcccgttc attatgcgga aaggggtaaa aggaggcaat cctgaaaacc
     1981 cgcttttcag gctaaagccg tgccgcaacc ggaaggagaa ccacccctat ccaacacatt
     2041 gaacctaaaa aagagcagtt gaaccttcaa cgctctttgt ttcaggtttc aaggtttcag
     2101 ggtttcaggg tttcatccaa atcggggtcg gggtcgggat cgctatcgga atcgattgtt
     2161 taaccacaga acactttctt gggtttcaag gtttcaaggt ttctgggttt ctgggtttcg
     2221 gggtttcatg gtttcagggt ttcagggttt ctgggtttca tccaaatcgg ggtcggggtc
     2281 gggatcgcta tcggaatcga tcgcacaggc taccggctat tgcaacacct tcaacgtttt
     2341 agttcagagg atgtt
//
LOCUS       DAOSRB010000312         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_41480_length_2347_cov_5.798429, whole genome shotgun sequence.
ACCESSION   DAOSRB010000312 DAOSRB010000000
VERSION     DAOSRB010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41480_length_2347_cov_5.798429"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..694
                     /locus_tag="PLZ74_11195"
     CDS             158..694
                     /locus_tag="PLZ74_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98925.1"
                     /translation="MIENNPLPLQSKENLERMLLISRYIREVKRAIWDGETTSFLIAI
                     PCALSSYNGMCHLLEGGWRLLCVFSLALSGSVCYDIAKLIIIQRVRRRWSYPQKSSLA
                     EWERDAAKKFDDAEACTLTRYPRPIQIIIMTMVIPSVIASVLYGAFIYVADQDHPERI
                     YLVIAAFALLDWYYRNDD"
     gene            complement(854..2089)
                     /locus_tag="PLZ74_11200"
     CDS             complement(854..2089)
                     /locus_tag="PLZ74_11200"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOR98926.1"
                     /translation="MYTVLRQLVQWIPSGMIQRLADEHGLDIRKWSAVSHVVALLYGQ
                     LAGCDSLNGIVDAARVHEGEWRNIRGAEPPKRNTFSNANRSRNADMAERLYWEVFEHL
                     VTVCPEFGEYRKHKGFLARMKRGIFAIDSSTIKLCLSCIDWARHRRRKAAAKLHLNLD
                     VGSRLPAFAVVEGAGHHDSTRAETLCADMKAGDVCLADRAYTDLAFLDGLRARGIFFV
                     VRQKRNMRFKCVKRLPKGRDASIVADEIVEPVLARSKALYAVPLRRVTARVEVDGKTM
                     EMVFLTDNFDWSARTVAELYRARWSVELFFKELKQTCLIRDFVGYNENAVRWQVWTGM
                     LAHLLLRFMRHVAGWTLSFSRLAGVVRAAVWVKRELVGILKPYGTAPPPKPGEPAAKP
                     PPLQAFFDFWRLDYGTASP"
BASE COUNT          431 a          686 c          714 g          516 t
ORIGIN      
        1 tctttccagt gtggcgtgtg ccactagtta ccgagcgaag cgaggttact cttgactggg
       61 gcatggagct tggatgtccg cgccccgcct ttttaagcgg cacgcggccc ggatcactaa
      121 ggcacaggtg tttagcactt ctttaaggat aagcgtcatg atcgaaaaca atccgcttcc
      181 tcttcaatca aaagagaact tggaacgaat gcttctgatt tctagatata tccgggaggt
      241 taagcgagca atatgggatg gtgagacaac ctcttttctg attgcgatcc cttgcgcact
      301 ctcatcctac aacggtatgt gtcatcttct ggaaggagga tggcgtcttt tgtgcgtgtt
      361 ttcgctggcg ctttccggca gtgtctgtta cgacattgca aagttgataa taatccagcg
      421 ggttcgcagg cggtggtcct atccgcagaa aagcagtttg gcagagtggg agagagatgc
      481 ggcaaaaaag ttcgacgatg cggaggcgtg tacattgaca aggtatccac ggcccattca
      541 aatcataata atgacaatgg tcattccctc ggtcattgct tcagtactgt acggcgcgtt
      601 catctatgtg gcggatcaag atcacccaga gagaatttat ctggtgattg ccgcgttcgc
      661 gcttttggac tggtactacc gcaatgatga ttagaaacag catggaaaaa ctgaagacgg
      721 atcactagca tcccataggt gacacaattc acccttaccc atgcggattt gctggggttt
      781 tcgaggttta ttttgaggtt acgggttgga ggttttcccg gcggggccgg ccgattcgcg
      841 ttttccttgg catttacggg cttgctgtcc catagtccaa gcgccagaag tcgaaaaagg
      901 cctgcaaagg cgggggtttc gcggccggtt cacccggttt gggcggcggt gctgtcccat
      961 agggtttgag gattccgacc agctcgcgct tgacccacac cgccgcgcgg accacgcccg
     1021 cgaggcgcga gaagctgagc gtccagcccg cgacgtgccg catgaagcgc agcagcaggt
     1081 gggcgagcat cccggtccag acctgccacc tgaccgcgtt ctcgttgtag ccgacgaagt
     1141 cgcggatcag gcaggtctgc ttaagctcct tgaagaacag ctcgacggac cagcgcgccc
     1201 ggtacagctc ggcgactgtc cgcgcgctcc agtcgaagtt gtccgtgagg aacaccatct
     1261 ccatcgtctt gccgtccacc tccacgcgcg cggtcacgcg gcgcaggggg acggcgtaca
     1321 gcgccttcga gcgggcgagg acgggctcga cgatctcgtc ggcgacgatg ctcgcgtcgc
     1381 gccccttcgg caggcgtttc acgcacttga accgcatgtt cctcttctgc cggaccacga
     1441 agaagatgcc gcgcgcccgc agtccgtcga ggaaggcgag gtccgtgtag gcgcggtccg
     1501 ccaggcacac gtcccccgcc ttcatgtcgg cgcagagcgt ctccgcccgg gtcgagtcgt
     1561 ggtgcccggc gccctccacg acggcgaacg ccggcaggcg gctgccgacg tcgaggttca
     1621 ggtgcagctt ggccgcggcc ttcctcctgc ggtgccgcgc ccagtcgatg cagctcaggc
     1681 acagcttgat ggtgctggag tcgatggcga agatgccccg tttcatgcgt gcgaggaacc
     1741 ccttgtgttt ccggtactcc ccgaactccg ggcagacggt gacgaggtgc tcgaagacct
     1801 cccagtacag ccgctcggcc atgtccgcgt tcctgctccg gttcgcgttg gagaacgtgt
     1861 tgcgcttcgg cggctccgcg ccgcggatgt tgcgccactc gccctcgtgg acgcgggccg
     1921 cgtccacgat cccgttgagg ctgtcgcagc ccgcgagctg cccgtacagc agggcgacca
     1981 cgtggctcac ggccgaccac ttgcggatgt cgagtccgtg ctcgtccgcc agccgctgga
     2041 tcatcccgga gggaatccat tggacgagct gccgcagcac ggtgtacact gtcttcgtcg
     2101 gcttcggggg ctcgaccccg agcaattggg gtattaactt tttcatgcgc ctgtaggcta
     2161 caggcttggg cctccgaagc cgacacttta ttgtgggatt ttcggggttg actctttcca
     2221 ccccccccta gcataataag tgtcaatcat aggaatcggc gtgttgccgg ctaagcctat
     2281 gggatgctag tgtttcaaaa aatgcttctc gttcgtttgc tgcacgtgcc ggtgatcgca
     2341 acggttc
//
LOCUS       DAOSRB010000313         2339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_41658_length_2339_cov_8.725044, whole genome shotgun sequence.
ACCESSION   DAOSRB010000313 DAOSRB010000000
VERSION     DAOSRB010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41658_length_2339_cov_8.725044"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLZ74_11205"
     CDS             <1..1209
                     /locus_tag="PLZ74_11205"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR98927.1"
                     /translation="ILEQVRKSYWTKSGLLQQLSHTLSPGQPDPFLAVRDKKRELYQT
                     IAKHLETYEFDIADLQLNLLLDIDDRDRFALVNKARLAIERRDLPEAGLWMDLAKENG
                     VTPAELIWHEAALLILDGKLPEARERLNTALNAAPADIGLWGLLAEILLQLEEYPELE
                     MRVFPVLRSASVKKEHYLMYKVRGYLLRHNGTEHYRAARANFLRALELNPNLNDVREE
                     ILRLDDVLEVPAFSEQDACAILRQNPDHAFANYLWGRVRLERGEVDLAEDLFRRSLET
                     ERNAPAFAGLGTVLLEKGEHALAEKFLRRALDDAPQRLDAWHALARLLLATGRADEAS
                     RALDAVLQAKPDDFSARLTLIRIRMAQRNLREAAEQISGLLEQEAHLPRHILRELKPL
                     SQQLLRDLAN"
     gene            1220..2119
                     /locus_tag="PLZ74_11210"
     CDS             1220..2119
                     /locus_tag="PLZ74_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR98928.1"
                     /translation="MSLLKKFAAYLEYLAIRFLGMFYGLVPASRAYDLGARLTALAYP
                     LFAARRRTAVENILKAHITDDPDEADRIARQAFGHLAGHLCESLKAGNVISDETWRDH
                     ITLDGPAESFALLLESPGVPILILTGHLGAWESAVPILSRARPMIAVARTLNNPFVER
                     FLKRHHFRGQITIIPKKKGFSPDVLRAWKNQGAAMTLVMDQHAGPKQGVTVDFLGRPA
                     STHTSPARLHLQTGAPILVGGFMREGPFRYRMVTGLPIRFQPTENREADVRALTAEIN
                     ARLGEVIRRYPEQYLWAHKRWRV"
     gene            complement(2196..>2339)
                     /locus_tag="PLZ74_11215"
     CDS             complement(2196..>2339)
                     /locus_tag="PLZ74_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOR98929.1"
                     /translation="LRLTLGADRVGTGVFGAEMKVRLLNDGPVTVELCTEVACPPPRT
                     SCG"
BASE COUNT          459 a          811 c          662 g          407 t
ORIGIN      
        1 attctggagc aggtccgcaa gagctattgg acgaagtccg gactgctgca gcagttgtcg
       61 cacaccctct cgcctgggca gccggacccc tttctggccg tacgcgacaa gaagcgcgaa
      121 ctctaccaga caatcgccaa acatctggag acgtatgagt ttgacatagc ggacctccaa
      181 ctcaacctgc ttctggatat cgatgaccgg gaccggttcg cgctggtgaa caaggcacgc
      241 ttggccatcg aacgccgtga ccttcccgag gccgggctct ggatggatct ggccaaggaa
      301 aacggcgtca ctcccgccga actgatctgg cacgaagcgg cccttctgat tctcgacggc
      361 aaactgcccg aagcccgcga acggcttaac accgcgctca acgccgcacc cgccgacatc
      421 ggattgtggg gtctgctggc ggagattctg ctgcaactgg aggagtatcc cgaactcgag
      481 atgcgcgtct tccccgtgtt gcgcagcgcc tccgttaaaa aagaacacta tctcatgtac
      541 aaagtccgcg gttatctgct ccggcacaac ggtacggaac actaccgcgc cgcacgcgcg
      601 aacttcctgc gagccttgga gttgaaccct aacctcaacg acgtgcgcga ggagatcctg
      661 cggcttgatg acgtgctgga ggtgcccgct ttcagtgaac aggacgcctg cgcgattctg
      721 cgccagaatc ccgaccacgc cttcgccaac tacctctggg gccgcgtgcg gctcgaacgc
      781 ggcgaagtgg acctcgccga agacctcttc cgccggagtt tggaaactga acgcaatgcg
      841 ccggcgttcg cgggccttgg cacggtcctg ctggaaaaag gcgagcacgc ccttgccgaa
      901 aagtttttgc gccgtgcact cgacgacgcg ccgcagcgcc ttgacgcctg gcacgcattg
      961 gcccgtctgc tgttggccac cggacgcgcc gatgaggcct cccgggccct cgatgccgtg
     1021 cttcaggcga aacccgatga cttctccgct cgcctgacgc tgatccgtat ccgaatggcg
     1081 caacggaacc tccgggaggc cgccgaacag atctccggtc tgctggagca ggaagcccat
     1141 ctgccccggc acattctccg ggaactcaaa cccctcagcc agcaactgct ccgtgacctc
     1201 gccaactaat ccgtgccgca tgagcctgct caaaaaattc gccgcttatc ttgaatatct
     1261 cgccatccgc tttctgggca tgttctacgg cttggtcccc gcatcgcggg cctatgatct
     1321 gggtgcgcgc ctgactgccc tcgcctaccc gctgtttgcc gcacgccgac gcacggctgt
     1381 cgagaacatc ctgaaggcgc atatcaccga cgacccggac gaagccgacc ggattgcgcg
     1441 tcaggccttc ggccatcttg cgggccacct gtgcgagtcc ctcaaggccg gaaacgtcat
     1501 ttcggacgag acctggcgcg accacatcac gctcgacggc cccgccgaat cgttcgccct
     1561 cctgctggaa tcgcccggcg tgccgattct catcctcacg ggccacctcg gcgcctggga
     1621 gtcggctgtc cccatcctct cccgtgcccg cccgatgatc gccgtggccc gcaccctaaa
     1681 caacccgttc gtcgaacgtt tcctcaagcg gcaccacttc cgcgggcaga tcaccatcat
     1741 ccccaaaaaa aaggggttct cgccggatgt gttgcgcgcg tggaaaaatc aaggggctgc
     1801 catgacactc gtgatggacc agcacgcggg ccccaagcag ggcgtgacgg ttgactttct
     1861 gggccgtccc gcctccaccc acacctcacc cgcgcgcctc catctccaaa ccggtgcgcc
     1921 aatcctggtc ggcggattca tgcgcgaggg gcccttccgc taccggatgg tcaccgggct
     1981 cccgatccgc tttcagccca ccgagaaccg ggaggcggat gtccgcgcac ttaccgcaga
     2041 gatcaatgcg cgactgggcg aagtgatccg gcgctacccg gaacaatacc tctgggcaca
     2101 caaacgctgg cgcgtgtgag cgccgcagac aagcggatat cgaaatcggg gccgggatcg
     2161 aaccccttgc gagggaatcg caaaaagaac tgacgttagc cacacgatgt gcgaggaggg
     2221 ggacacgcaa cctccgtgca caactcgacg gtcaccggcc cgtcattcaa cagacgcact
     2281 ttcatctccg cgccgaacac acccgttcca acgcggtccg cgcccagagt caggcggag
//
LOCUS       DAOSRB010000314         2328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_41947_length_2328_cov_11.306203, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000314 DAOSRB010000000
VERSION     DAOSRB010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41947_length_2328_cov_11.306203"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..398)
                     /locus_tag="PLZ74_11220"
     CDS             complement(123..398)
                     /locus_tag="PLZ74_11220"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOR98930.1"
                     /translation="MDDTLFTRAKDVELREIVGEHFLIVLHAGESKMFSLNDMGLWFW
                     QQLERPVTKAELLEAMLEEYEVDDETARAEIDRFLAHLVERGLASRI"
     gene            complement(410..>2328)
                     /locus_tag="PLZ74_11225"
     CDS             complement(410..>2328)
                     /locus_tag="PLZ74_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOR98931.1"
                     /translation="VASDFMLLAATVHYPRYYTAQEGMLQCTGGEGPVIAAGGFTTQN
                     LAVWEVTDAAQPIALSDVCVVPDGEGGWQALFACGGAAARYVVFDRSQGAFEPSVSGA
                     RVIDWSALDEMPELAIVTPPRRWWPGFAEAAAPLAEFREAQGLRARVIDAEDLYNAFT
                     DGLVHPEAFRRFSAAGVAQADGQVLRYLLFAGHGGSDYKLEVFPPGKAGAYPALFPLY
                     LVSQVDAAANGALLLPNDPVLGDVQGDAVPEVAIGRFLATNALELARMVEKTIRYELT
                     ETWKRKAVFSCDWQNTGSKYVNFTGISESIAVSFPSVGWWLETFYPAPDQSNPGPLWR
                     DTYYGTGISYEFQEGAGFYYFIGHSSDTIAGNTAANKLFNASTLQTASWPFAPVALLL
                     GCRMGRWTALDLRQEQQTIAEAGVRNPISGFVAVISAAGYMATADATAFSQAFGAQVA
                     AGARRLGDIWCGTFAALGGDRALALRHMVFLGDPSLNMRPDQTARGTPTDWLLAQGLR
                     GDPYADLLDQDGDGFATWIEAQAGTGALAGGMRISRLVLPQAGTYDPLAVVPDSAAPG
                     LALNVETLAGIAFRVMTTDDLRSTSWVPLPWRPAGGGAWSTEPIPGDWPLKQIEVPFD
                     RNVQRRFYRPVAER"
BASE COUNT          493 a          854 c          585 g          396 t
ORIGIN      
        1 tgtacgcggg tcgcggcacc gctttccggc ccgtctacgc caaagcggcg tcgtgaggaa
       61 taccgcttgc cgccgcactc caaatctatt cgcttgagct cctaaatcct aaatcccaaa
      121 tcctaaatcc tactcgccaa gccccgctca acgagatgcg cgagaaaacg gtcaatctcc
      181 gcacgcgctg tctcgtcgtc gacctcgtac tcctccagca tcgcctcaag cagttcggct
      241 ttcgtgaccg gtcgctccaa ctgctgccaa aaccagaggc ccatgtcgtt caggctgaac
      301 attttggact caccggcatg caacacgatg agaaaatgtt cgcccacgat ctccctcaac
      361 tcaacatcct tcgcccgtgt aaaaagcgtg tcatccatag tgatacttcc tagcgttctg
      421 cgaccggtct gtaaaagcgg cgctgtacat tcctgtcgaa cggcacctca atctgcttga
      481 gcggccaatc acccgggata ggctcggtcg accatgcgcc cccccctgcc ggccgccaag
      541 ggagcggcac ccacgacgta cttctcagat catctgtcgt catcaccctg aatgcaatac
      601 cagccagcgt ttcgacattc agcgcgaggc ccggtgcggc agaatccggc actacagcca
      661 gcggatcgta ggttccggcc tgcggcagca ctaatcggct gatccgcatc ccgcccgcaa
      721 gcgccccggt gccggcctgc gcctcgatcc acgtcgcaaa cccgtcgccg tcttggtcga
      781 gaaggtccgc atagggatca cctctcaatc cctgcgccag cagccaatcg gtcggcgtcc
      841 cgcgcgcggt ttggtcgggt cgcatgttga gcgacgggtc acccaagaac accatgtgcc
      901 gcagggcaag cgcccggtca cctcccagcg cggcgaaagt cccgcaccag atatcaccca
      961 gccgacgcgc accggccgcc acctgagcac caaaggcctg cgaaaacgcg gtggcatccg
     1021 ccgtcgccat atatccggca gcgctgataa cagccacgaa accggatatc gggttcctga
     1081 cacccgcctc ggcgatcgtc tgctgctctt gcctcagatc aagcgccgtc caacgtccca
     1141 tacggcatcc caacagcaac gccacgggcg cgaacggcca ggaggcggtc tggagcgtcg
     1201 aagcgttaaa cagcttgttc gccgcagtgt tgcccgcaat ggtatcactg gaatggccga
     1261 tgaaataata gaaacccgcc ccctcctgaa actcatacga aatgcccgtt ccataatacg
     1321 tgtcgcgcca taacggtccc ggattactct gatccggcgc gggataaaag gtttcgagcc
     1381 accaccccac gctcggaaaa gatacggcga tggactcgga aatcccagtg aaattcacat
     1441 atttcgagcc cgtattctgc cagtcgcaag agaacaccgc cttgcgtttc cacgtctcgg
     1501 tcaactcata ccggatggtc ttctccacca tgcgcgccag ttccagcgcg ttcgtcgcca
     1561 aaaaacgccc gatcgccacc tccggcaccg cgtccccctg cacatcaccc aataccggat
     1621 cattcggtaa caacaacgcg ccgtttgccg ccgcgtccac ctgcgacacc aaatacagcg
     1681 gaaacaaggc tgggtaggcc cccgccttgc caggcgggaa tacctccagt ttgtaatcgc
     1741 ttccgccatg cccggcaaac agcagatagc gcagcacctg accgtccgcc tgcgccacac
     1801 ccgccgcact aaagcggcgg aaggcctccg gatgcaccaa gccgtccgtg aaggcgttgt
     1861 agagatcttc cgcgtcgatc acacgcgcgc gcaacccctg cgcctcgcga aactcggcca
     1921 gcggtgccgc cgcttccgcg aaccccggcc accaccggcg cggcggtgtc acgatcgcca
     1981 actcgggcat ttcgtccagg gcactccagt cgatcacacg cgcaccactg acggacggtt
     2041 caaaagcgcc ctgcgaccga tcgaaaacca catagcgcgc cgccgccccg ccacatgcaa
     2101 acagcgcctg ccagcccccc tccccgtccg gcaccacgca cacatcggac aacgctatcg
     2161 gttgagccgc gtccgtcacc tcccacaccg ccagattctg tgtcgtgaaa ccacccgccg
     2221 caatcaccgg gccctccccg cccgtgcact gcagcatgcc ctcctgcgcc gtgtaatatc
     2281 tcgggtagtg cactgtcgcg gccagcaaca taaaatcgct ggcaacct
//
LOCUS       DAOSRB010000315         2326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_41995_length_2326_cov_4.912373, whole genome shotgun sequence.
ACCESSION   DAOSRB010000315 DAOSRB010000000
VERSION     DAOSRB010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41995_length_2326_cov_4.912373"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLZ74_11230"
     CDS             <1..633
                     /locus_tag="PLZ74_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98932.1"
                     /translation="GYGFVDFIRRILNTEAKVRALTTKMQQSQTLQPDEVFELALAYR
                     DLGQNDAAAKYLEQLMTVPNLPVTRMFEIGSLLSGLKRGESACKAMDIVMARMPENMP
                     PEQLLEVVRVYGEAGNADKMAAPLSRYLQLRPNDWQAWLDMATLYAMKRQQQPMQFAL
                     QKAINLGKMQAVQRIQENTILRQVAGPLLQQMMQQQGPAGLPPFGPVRGR"
     gene            644..2248
                     /locus_tag="PLZ74_11235"
     CDS             644..2248
                     /locus_tag="PLZ74_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HOR98933.1"
                     /translation="MRLGKAWEALLCIGVLAGALALRLPHLAQRPMHGDEANQAVRTG
                     QLLEEGVYHYDPTDHHGPSLYFAAWFFCRATARTFADTTEWSYRLVPVTFSLLTLLIM
                     FAMGVSERQGLFVNRSARFTALLGMAVSPAMVYYSRFFIQETMLVTFLAGMLLCAVRY
                     AGLRAAEVPGERRSGRRTLWAAAGFGAFAGLAAATKETAVLSFAAAAVATVAACGIGR
                     LRRVWRTRDAVLAAGVAIGVAALLFSSFFTYPRGIVDALFSTAQAYLKRATAVPEHQH
                     PWNFYLKILFWFRYGRGPLWSEAALLVPALIATVTAFLRFPDASEEETRVLRWTRFLA
                     VYTLVLTALYSAIPYKTPWCALSFLHGVILLAGMGVGQAVAWLARQNIRGVRLAGTVV
                     VWALVAFVAARHAAQAYRACFRLPADPRNPYVYAHTGTDALNLVAAIEEAARAAQGTD
                     TPIAVAVPVPDTWPLPWYLRAYTRVGYWTRIEQIPESFHPVVVIAAADQGDVADARFG
                     TGRRASFYGIRPGVLLNLFVPVPSAP"
BASE COUNT          368 a          661 c          808 g          489 t
ORIGIN      
        1 gggtacgggt ttgtggattt catccggcgg attctgaaca ccgaggcgaa agtgcgcgcc
       61 ctgaccacca agatgcagca gagccaaacc ctgcaaccgg acgaagtgtt cgaactggcg
      121 ctcgcgtatc gggacctcgg gcagaacgat gcggccgcga agtatttgga gcaactcatg
      181 actgttccca atctgccggt gacgcggatg tttgagatcg gttcgctgct cagtggtctg
      241 aagcgggggg aaagcgcttg caaggccatg gatatcgtga tggcgcgcat gccggagaat
      301 atgccgcccg agcagttgct ggaggtggtc cgggtgtacg gggaggcggg gaacgcggat
      361 aaaatggccg cgccgctctc acgctatctc cagttgcggc cgaacgactg gcaggcgtgg
      421 ctggacatgg cgaccctcta cgccatgaag cgtcagcagc aaccgatgca gttcgcgctg
      481 cagaaggcga tcaatctggg gaagatgcag gccgtgcagc gcattcagga gaacacgatc
      541 ctgcggcagg tggccggacc gctgctgcag cagatgatgc aacagcaagg gcctgcggga
      601 ttgccgccgt tcggtccggt ccgggggcgg tgaggtgtcc gggatgcggt taggaaaagc
      661 gtgggaagca ctgttgtgca tcggagttct ggcgggtgcc ttggcgctgc gtttgcccca
      721 tctggcacaa cgtccaatgc atggggacga agcgaatcag gcggtgcgaa ccggtcagtt
      781 gctggaggag ggggtctatc actatgaccc cacggatcac cacggcccgt cgctctactt
      841 cgcggcctgg ttcttctgcc gtgcgaccgc acgaaccttt gcggacacca cagagtggtc
      901 gtaccgtctc gtgcccgtga cgttcagcct gttgacgctg ctgatcatgt ttgccatggg
      961 ggtgtcggaa cgacagggtc tgtttgtgaa ccggagtgcg cgtttcactg cgttgctcgg
     1021 catggcggtc tcacccgcga tggtctacta cagccggttt ttcattcaag aaacgatgtt
     1081 ggtcaccttt ctcgcgggta tgttgttgtg cgcggtgcgc tatgcggggt tgcgtgccgc
     1141 cgaggtgccg ggggaacggc gctccgggcg tcggaccctg tgggctgcgg cggggtttgg
     1201 tgccttcgcg gggctggcgg cggcgaccaa ggagacggcg gtgctttcgt ttgccgcggc
     1261 ggcagtggcc accgtggcgg cctgcgggat tggccggttg cgccgcgtct ggcgcacacg
     1321 cgatgcggtc ttggccgccg gggtcgcgat cggggtggcg gctctgctct tttcatcctt
     1381 tttcacctat ccgcgcggaa tcgttgacgc gctcttctcc acggcgcagg catatctgaa
     1441 gcgtgccacc gccgtgccgg aacatcaaca tccctggaat ttctatctga agattctctt
     1501 ctggttccga tacggacggg gaccgctttg gagcgaggcc gccctgttgg tgcccgcgct
     1561 gatcgcgacg gtaacggctt tccttcgctt tccggacgcc agtgaagagg agacgagggt
     1621 tttgcgctgg acacgcttcc tcgcggtcta cacccttgta ttgacggccc tgtacagcgc
     1681 gattccgtac aagaccccct ggtgcgcgct ttcatttctg cacggggtga ttctgttggc
     1741 gggcatgggc gtggggcagg cggtggcgtg gctggctcgt caaaacatac ggggagttcg
     1801 ccttgcgggt actgtcgtgg tgtgggcgct ggtcgcgttc gtggccgccc gacatgcggc
     1861 acaagcctac cgggcttgtt tccggttgcc tgcggatccg cgcaatccct atgtctacgc
     1921 ccatacgggc acggacgcgt tgaatctggt ggcggcgatt gaggaggccg cgcgggcggc
     1981 acagggaacc gacacgccga ttgccgtggc ggttcccgta cccgacactt ggccgttgcc
     2041 ttggtacctc cgcgcctata cgcgcgtggg gtattggacg cgcatcgaac agataccgga
     2101 gtcgttccat ccggtggtgg tgatagcggc ggcagaccag ggggatgttg cggacgcacg
     2161 tttcggcacc gggcgccgcg cgtcctttta cgggatccgt ccgggtgtgc tgctgaacct
     2221 gttcgtgccc gtgccctccg ctccgtgagg cggcgcacag cggccagcgc gtttaaccta
     2281 aaaagagcag ttgaaccttc aactctcttt ggtttcaagg tttcag
//
LOCUS       DAOSRB010000316         2313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_42315_length_2313_cov_6.539858, whole genome shotgun sequence.
ACCESSION   DAOSRB010000316 DAOSRB010000000
VERSION     DAOSRB010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42315_length_2313_cov_6.539858"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..357)
                     /locus_tag="PLZ74_11240"
     CDS             complement(220..357)
                     /locus_tag="PLZ74_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98934.1"
                     /translation="MANVHVLQIAIGIAIGFEIDCCAHAVARGDKCNDGGDPDGAAME
                     P"
     gene            503..1267
                     /locus_tag="PLZ74_11245"
     CDS             503..1267
                     /locus_tag="PLZ74_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOR98935.1"
                     /translation="MMTVTLSALESLLDETLEIGRFRDASNNGLQIANNGSVTRVVAG
                     VDASLRLLEAAAARGADAVVCHHGVSWGDSLKRITGLNHRLVSFALAHNLAVYAAHLP
                     LDAHPRYGNNARLCKALGLRRPVPAFTYHGETIGFVAAYERAMPLERFFKRVQKVVAP
                     ACRLLPFGKRSIRTVGVVSGGAADMIDQAAAHGVDAYLTGESSLQGYNLAEQLGQTVL
                     FAGHYATETFGVRALAELIRRRLGIPAEFIDFQIEY"
     gene            1297..2010
                     /gene="rnc"
                     /locus_tag="PLZ74_11250"
     CDS             1297..2010
                     /gene="rnc"
                     /locus_tag="PLZ74_11250"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOR98936.1"
                     /translation="MPEGKTDPLDAFEARLGYVFRDRELLRTALTSPSFRAGRVVGAI
                     PDNQRLEFLGDAVLGLLAAQSLYDRYLAEDEGNLTVRRSLQVNRGALANLARRLGLGA
                     CLRIGQREEENGARDQDRMLADALEAVFGAIWREGGWDAVWRVYRRLDPEEEEAVPWR
                     GNPKGALQELAQRHAWPNSPTYELIGRSGPDHGPVYTVRVRVAGGHEAEGEGRTKRAA
                     ETAAAQALLCRLAPPESLV"
BASE COUNT          448 a          667 c          759 g          439 t
ORIGIN      
        1 gtctcgggac gccctcggtg tccagtattt gacggatcgt caccgcctca aaaaacctac
       61 ggtgtctttt ctatttgacg gaccaccccc gtactctcgc aaaaccatgc cccctcactt
      121 tgttaaagta aggtttataa aaaccgggct gttttgccat tcgggaaggc gtccctaccg
      181 taatcggtgc gcgacagggg tgtgcgaagg tcatgcgttt cagggttcca ttgcggcgcc
      241 atcgggatcg ccgccatcat tacacttgtc gccgcgggcc actgcgtggg cgcaacagtc
      301 gatttcgaac ccgatagcga taccgatagc gatttggaga acgtgaacgt tggccatgcc
      361 ccatacccgt cctcgttcac gtacccgtac acgatgactc ccccccctgt cacgacccga
      421 taccgttccc ttgcgttcca caggtaaaac gattacatta taaatcacat ggggcagtgc
      481 aggaatcgct gtccgggata aaatgatgac ggttacccta tcggcgttgg agagcctgtt
      541 ggacgagacc ctggagatcg gccgttttcg tgacgcgtcg aacaacgggt tgcaaatcgc
      601 taacaacgga agcgtgacgc gcgtggtcgc cggggtggac gccagtctgc gcctgttgga
      661 ggccgcggcg gcccgggggg cggacgcggt ggtttgtcat cacggcgtga gctggggcga
      721 ctcgctcaag cggatcaccg ggctgaacca ccggctggtc tccttcgcgc tcgcgcacaa
      781 cctcgcggtc tatgcggcgc acctgccgct ggacgcccat ccgcgctacg gtaacaacgc
      841 gcggctctgc aaagccctcg ggttgcggcg gccggttccg gcctttacct atcacgggga
      901 gaccatcggc ttcgttgccg cctacgagcg ggcaatgcca ttggaaaggt ttttcaagcg
      961 ggtgcagaag gttgtggcgc cggcctgccg cctgttgccc ttcggcaagc gtagtatccg
     1021 gacggtaggc gttgtttcgg gcggggcggc ggacatgatc gatcaggctg ccgcacatgg
     1081 ggtcgatgcc tatcttacgg gggagtccag tctgcagggg tataacctgg ccgaacagtt
     1141 ggggcagacc gtgctgttcg ccgggcatta tgccaccgag acgttcggtg tccgggcgtt
     1201 ggcggaactc atccggcggc ggctcggcat cccggccgag ttcatcgatt tccagatcga
     1261 atattgaccg tcgcgggaaa aggggagggg cgaccggtgc cggaaggaaa gacagatccg
     1321 ctcgatgcgt tcgaggcgcg gttgggatac gtcttccgcg accgggaact gctgcgcacc
     1381 gcgttgacct ctccctcctt ccgtgcgggg cgtgtggtgg gcgcgattcc ggataaccaa
     1441 cgactggaat ttctggggga tgcggttctc ggcctgctcg ccgcgcagag tctctacgac
     1501 cgttatctgg ccgaggatga gggaaacctc acggtgcggc gcagtctgca ggtgaaccgc
     1561 ggggcgctcg ccaacttggc gcgtcggctc gggctcggtg cctgtctacg gatcggtcag
     1621 cgcgaagagg agaatggcgc gcgcgatcag gaccgtatgc tcgccgacgc gctggaagcg
     1681 gttttcggcg ctatatggcg cgagggcgga tgggacgcgg tgtggcgcgt ctaccggcgg
     1741 ctggacccgg aggaagagga ggccgtgcct tggcgtggca atcctaaagg ggcgttgcag
     1801 gaattggcgc agcggcacgc ctggccgaat tcgccgacct atgaactgat cgggaggagc
     1861 ggtccggacc acgggccggt ctacaccgtg cgggtacggg tggcgggagg gcacgaggca
     1921 gaaggagagg gccgcaccaa acgggccgcg gagacggctg ccgcgcaggc gctgctttgc
     1981 cgtctggcac cgccggaatc ccttgtgtaa gcgaggagaa aggctgtgag actgtgagac
     2041 tgtgaggctg tgaggctgtg gttaagcaag cgatcccgat agcgatcccg accccgatcc
     2101 cgatttggat gaaaccctga aacccagaaa ccctgaaacc cagaaacctt gaaaccaagg
     2161 agagtgtccc gtggttaaac aatcgattcc gaaagcgatc ccgaccccga tcccgatttg
     2221 gatgaaaccc tgaaaccaga aaccttgaaa ccctgaaacc agaaaccccg aaaccttgaa
     2281 accaaggaga gttgaaggtt caactgctct ttt
//
LOCUS       DAOSRB010000317         2311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_42363_length_2311_cov_5.990248, whole genome shotgun sequence.
ACCESSION   DAOSRB010000317 DAOSRB010000000
VERSION     DAOSRB010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42363_length_2311_cov_5.990248"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..2251
                     /locus_tag="PLZ74_11255"
     CDS             44..2251
                     /locus_tag="PLZ74_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOR98937.1"
                     /translation="MLCRMSRLGVLGVVLLGSRGIFASERQTLSLDGEWEIADSLLPE
                     VIPAAFPHTVPVPGLANLAKPAFPDVDRFISRENLSRRILHKLVPDDWLEKYWVGKVE
                     QDRNYFWCRRTFRAPKERAAALLKINKAQFGTAVWLNGLKVGESPGCFTASVFALGKA
                     IRWNAENTLVVRIGAHPAVLPDTFPTGSDFEKNTWSPGIYDSVSVSFCDNPVIESIQI
                     APRIASGEALVQTRVKNHSDAPVNASVFHTVKTWKRAKPVAMGEAVAVTLPPGGERVL
                     TQTIQIPDARLWSPEDPFLYVVETHTGGDSVATRFGMREFRFDAATGRAYLNGRVYFL
                     RGSNITLHRFFEDPLCKALPWNEKWVRKLLGEVPKKMHWNYFRFCIGPVPDRWFDICD
                     EVGLLIQNEFFVWTGAPGWDKRYSRTHDNAEMIRQYTDWMRDHWNHPSVVVWDANNET
                     MDASFGDIIIPAVRPLDLSNRPWENSYNKPVAPDDPVEDHPYLMIGGRHKPLKFKMSD
                     LETMDGKPLDKLPSDTNPKIINEYGWLWLDRDGTPTLLTENVYAQLCGTNSTARERLD
                     LYAYLLAGKTEFWRAHRQYAGIIHFVYLTCGYPGVYTADHFADVTRLKLDPAFADYMS
                     EAFKPLGVYVNFFQPTLKAGETRVFKVMLVNDESIPLSGQITLELIRRGKSSVLVRAE
                     QPFQIKPAGTASVAIALDMPDTEGECVLKAVAAADQKKDVSPTVCRRWVRLAR"
BASE COUNT          449 a          699 c          722 g          441 t
ORIGIN      
        1 ccggtcaaag gaacggtgat aaggcttaac taaggataaa aacgtgctgt gtcggatgtc
       61 gcgtctcggc gtgttgggcg ttgtattgtt ggggtctcgc gggattttcg cgtctgagcg
      121 gcagacgttg tcgctggatg gtgaatggga gatcgctgac agcctgctac cggaggtgat
      181 ccccgcggcg ttcccgcaca cggtgcctgt gcccggtttg gccaatttgg ccaaaccggc
      241 cttcccggat gtggaccggt tcatcagccg cgaaaacctg tcccgccgta ttctccataa
      301 gcttgtccct gacgattggc tggaaaagta ctgggtgggc aaggtggagc aggaccgcaa
      361 ctatttttgg tgccgacgga ccttccgcgc gccgaaggaa cgcgccgcgg ccctgctcaa
      421 gatcaacaag gcgcagttcg gcaccgccgt ctggctgaac gggttgaagg tgggcgagtc
      481 ccccggttgt ttcacggcca gtgttttcgc gctcggcaag gcgatccgct ggaatgccga
      541 aaacacgctt gtggtccgca ttggcgcgca tccggccgtg ttgccggaca ccttcccgac
      601 cggttcggat ttcgaaaaga acacatggtc gccgggcatc tacgacagcg tgtcggtgag
      661 tttctgcgac aaccccgtga tcgaatccat ccagatcgcg ccgcgcatcg cctcgggtga
      721 ggcgctggtt cagacccgcg tgaaaaacca ctccgacgcg ccggtcaacg catccgtgtt
      781 tcacaccgtg aagacctgga agcgggcgaa accggtcgca atgggtgaag cggtcgccgt
      841 cacgctgccg cccggcgggg aacgggtgct gacgcagaca atccaaattc ccgacgcgcg
      901 tctctggtcg ccggaggatc cgttcctgta cgtcgtcgag acgcacacgg gcggcgattc
      961 ggtcgcgacg cgcttcggca tgcgcgagtt ccgttttgac gccgccaccg gacgcgcata
     1021 tctgaacggc agggtctact ttctgcgcgg ctcgaacatc accctgcatc ggttcttcga
     1081 agacccgttg tgcaaggcgc tgccgtggaa tgagaagtgg gtgcgcaaac tcctcggtga
     1141 agtcccgaag aagatgcact ggaattattt ccggttctgc atcggtccgg tgcctgaccg
     1201 ctggttcgac atctgcgacg aggtcggcct gctgatccag aacgagtttt tcgtctggac
     1261 cggcgcgccg ggctgggaca aaaggtactc gcgcacgcat gacaacgccg agatgatccg
     1321 gcagtacacg gactggatgc gcgaccactg gaaccatccc agcgtcgtgg tctgggacgc
     1381 gaacaacgag accatggacg caagtttcgg cgacatcatc attccggccg tgcgtccgct
     1441 cgacctgtca aaccgtccct gggagaacag ctacaacaag ccggttgcgc cggacgatcc
     1501 cgtggaagat cacccgtatc tcatgatcgg cggccggcac aaaccgctga agttcaagat
     1561 gagcgacctg gagacgatgg acggcaaacc gctggacaag ctgccttcgg acacgaaccc
     1621 gaagatcatc aacgaatacg gctggctctg gctcgaccgc gacggcacgc cgacactcct
     1681 cacggagaac gtgtacgcgc agctctgcgg gacgaacagc accgcccgcg aacggctcga
     1741 cctctacgca tatctgctgg cgggcaagac cgagttctgg cgcgcgcacc gccagtacgc
     1801 cggcattatc cacttcgtct acctgacgtg cggttacccg ggtgtctata cggcggacca
     1861 cttcgcggat gtgacccggc tcaagttgga cccggccttc gctgactaca tgagcgaggc
     1921 attcaaaccg ttgggcgtct acgtgaattt cttccagccc acgctgaagg cgggcgagac
     1981 gcgtgttttc aaagtcatgc tggtcaacga cgagtcgatt ccgctgagcg gacagatcac
     2041 gcttgaactg atccgccgcg gtaaaagcag cgttctggtc cgtgcggaac agccgtttca
     2101 gattaagcca gccggtacgg cttcggttgc gatcgccttg gatatgccgg ataccgaggg
     2161 ggagtgcgta ctgaaagcgg ttgccgccgc agaccagaaa aaagacgtga gcccgacggt
     2221 ttgccgccgt tgggtgcggc tggcgcggta gccccatatc acgccctctt accgctgaat
     2281 gcgtcctgtt ccgccgcctg cggccagtgc c
//
LOCUS       DAOSRB010000318         2306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_42481_length_2306_cov_9.649045, whole genome shotgun sequence.
ACCESSION   DAOSRB010000318 DAOSRB010000000
VERSION     DAOSRB010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42481_length_2306_cov_9.649045"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(440..>2306)
                     /locus_tag="PLZ74_11260"
     CDS             complement(440..>2306)
                     /locus_tag="PLZ74_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOR98938.1"
                     /translation="SSVTDIEWDAFDGCASLTSVTIPASITYIGGYAVSGCEMLGGVY
                     FRGDAPFYGSDDSPFDNTPDVTVYRLAGKKGWPDVPNPWWGRPTALWSEVLSVTPGTH
                     AVAREAGTLTFSVVKMEPGTILSWTAAVISGGAWARITSGASGGDAGTITVGFDANLP
                     GGAQRTATIRVSAPDVPGSPVDVTVVQDENPGGGFLCDPSGDAALTATGTYDGYLYAD
                     RAFGDEAWPTVVGTLNVKLSKLDGRLTAKATLQAGRVSFSGKQRIERDEDGTHYSMLT
                     ARSGERLDLFVRQNRIWGTLAGGTFGTDEVFLEGARNRFADRRDAAAQAVLNRYKGYY
                     TACLAGAEFIQGELNAAPRGYGYLTLTVSNGGKVKIAGVLADGTKVSQSAPLLYFADC
                     GEWLCVPLFVPLYRKAGYVGGLFWIDPETREVYCGAETGESVRWENPGKGPDGFSGLL
                     DMRGGAYDRPPVLQTRYAFGAELDDDICAYHAAGEAWGCEFWPDEVPVDVTGSRMTIA
                     RGDRPVRIREDGETWYEYGGVNPNMVTLSFTARTGIFKGNFTLYYDYTDERDRFQHKT
                     VRVPYVGVMLQDPETGWLESGHGHCLFPSKDPELRAYRIQPSFPVELRGQGIDPQ"
BASE COUNT          415 a          769 c          701 g          421 t
ORIGIN      
        1 taagggaaaa aggcggagcg cgtcaagcgg aaaagaacgt ggaacatcga aaatcgctgt
       61 cgggatcggt gtaccgttcg tgtacgcaaa cgtggagcgt tgcgttataa acgactttcg
      121 ctgcgcacag cgcggccggg caagtgacac ttgtccctcc cggtgcttcg cgagcccagt
      181 cacaggacgg gagggacgag cgttgctcgt ccgggcggcg ctccgcgccg ccacacgggc
      241 aagatgcccg tgccacatag cttggcaccg gccattcgcc tgtcttgcgc ggcaccgccg
      301 cttctgaacg ctgaacgtcg aacgtcgaac gcagaagtca aaaaactcct aaatcctaac
      361 tcctaaatgt taactccgcg cgcagcgcgg aagggaaagg cttgaagcga ccacagaaga
      421 agggggatac cgtacggact tactgtggat caatcccctg tccccgcaac tcgactggga
      481 acgacggctg gatcctgtac gccctcagtt ccggatcttt ggacgggaac agacagtgcc
      541 cgtgtcccga ttccaaccag ccggtctccg gatcctgtag catcacgccg acgtagggga
      601 ctctgacggt cttgtgctgg aagcgatccc tttcgtcagt gtagtcgtaa tatagcgtga
      661 agttgccttt gaatatgccc gtgcgagccg tgaaactcaa agtgaccatg ttggggttca
      721 ccccgccgta ctcataccaa gtctcgccgt cctctctgat tctcacggga cgatccccgc
      781 gcgcgatggt catccgggat cctgtgacgt ccacgggcac ctcatccggc cagaactcgc
      841 atccccaagc ctctcctgcg gcatggtaag cgcagatatc gtcatccagt tcggctccga
      901 acgcataccg tgtttgcagg acaggcggtc tgtcgtaggc gccgccccgc atgtcgagca
      961 gtccggagaa gccgtcgggg cccttgcccg gattctccca cctcacagac tcgcctgtct
     1021 ccgcgccgca ataaacctcg cgcgtctcgg ggtcgatcca gaacagcccg ccgacgtacc
     1081 ccgccttgcg gtagagcggg acgaagagcg gcacgcagag ccactcgccg cagtccgcga
     1141 agtagagcag cggggcggac tgcgatactt ttgtgccgtc tgccagaacg cccgcgatct
     1201 tgactttgcc gccgttgctg acggtcagcg tcaggtatcc atatccccgc ggcgcggcgt
     1261 tcagctcgcc ctggatgaat tccgcgcccg ccagacacgc cgtgtagtaa cccttgtagc
     1321 ggttcagcac ggcctgcgcc gccgcgtccc tacgatccgc gaagcggttc cgcgcgcctt
     1381 caaggaagac ttcgtcggtg ccgaacgtcc cgcccgcgag cgtgccccag atgcggttct
     1441 ggcgcacgaa gaggtcgagc ctctcgccgc tccgcgcggt cagcatggaa taatgggttc
     1501 catcctcgtc ccgttcgatc cgctgcttgc cgctgaaact gactctcccg gcctgaagcg
     1561 tagccttggc ggtcagtctg ccgtccagct tgctgagttt gacgttcagc gttcccacca
     1621 ccgtcggcca cgcctcgtct ccgaacgccc ggtccgcata gaggtacccg tcgtaggttc
     1681 cggtcgccgt cagcgctgcg tcgccagacg gatcgcacag gaagccgccg ccggggttct
     1741 catcctgcac caccgtcacg tcgaccgggc ttcccggcac gtcgggcgcc gacacgcgga
     1801 tcgtcgcggt gcgctgtgcg ccgcccggaa gattcgcgtc gaacccgacc gtgatcgtgc
     1861 ctgcgtcgcc gccgctcgcg ccggaggtga tccgcgccca cgcgccgccc gatatcaccg
     1921 ccgccgtcca agagagtatc gtgccgggtt ccatttttac cacgctgaaa gtgagggtgc
     1981 ccgcttccct cgccaccgca tgggtgccgg gcgtcacgga caggacttcg ctccacagcg
     2041 cggtgggacg gccccaccag gggttcggta catccggcca gccctttttc cccgccagcc
     2101 ggtagacggt cacgtcgggg gtgttgtcga aaggactgtc gtcactgccg tagaaagggg
     2161 cgtcgccacg aaaatagacg ccgcccaaca tttcgcaacc ggagacggcg tagccgccga
     2221 tgtaggtgat gctggcgggg atggtgacgg aggtgaggga cgcgcaaccg tcgaaggcgt
     2281 cccattcgat gtctgtgacg ctactg
//
LOCUS       DAOSRB010000319         2240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_44195_length_2240_cov_5.943707, whole genome shotgun sequence.
ACCESSION   DAOSRB010000319 DAOSRB010000000
VERSION     DAOSRB010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44195_length_2240_cov_5.943707"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2001
                     /locus_tag="PLZ74_11265"
     CDS             <1..2001
                     /locus_tag="PLZ74_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98939.1"
                     /translation="GMKKSGWQMFMASLVGAWCALAGEPYYQTAFETAEEIRGWKRDA
                     NWKIEEGAGTGGSRALVWTNDDPKNYNVNSFTIPGGRPGLYFVATFKVKPEKITGARL
                     GCTICWTGKDKWLGGAGGTVMKLGDKRLKPDADGWYEMTIRMPSERLPEEATAVQLQL
                     YVHAGGTGRVAFDDIRVDTFADPKKAAVKTLTTSCYRDMAATGIVHVRAGLELPDKYL
                     GEKGLVTELAYRASDGARRQDAMRAVEAKQFVASIPVTSFAMGQHPLVATLKLENGEV
                     LGEKTLVFERVDTLPPRRVCFDRYGRTLVEGKRFFPLGMYWSENTLAKSNSLERYASG
                     PFNCLQTYEKQMTPEILDRYWRHGLRVVASVKDIYVRPVGGPLIGFTPPEVKTRTDET
                     NYVKRVVERCRNHPALLAWYTCDEIPHEYADRVEDRYSLLKQLDPDHPTFICLNHPVT
                     ARVFIDCTDVVGSDPYPIPGSDRVNTVLPPDAGAVWRAGDSARTIRESVFGLRPMWQV
                     PQAFAWKWDKSEQPELRFPTRRELSSMTWQQIAEGANGLLFYSYGQMMNKAGAELEEN
                     FRTTCSVAVEVRDLIPILLLEPGPDVRKMPPRTRVRTWKDGKKVYVLVCNTHPDRRVG
                     QVEIEGAYTTCAPVFGSGVTRAGGVLSLDMDSMGTAVVRLEP"
     gene            1998..>2240
                     /locus_tag="PLZ74_11270"
     CDS             1998..>2240
                     /locus_tag="PLZ74_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98940.1"
                     /translation="MTRVIKALSRNKLVGREKQVFAECNRKLEEARKHRAQARQSATG
                     ATPCTCRFKMLTRPADQDHVDNAIRAVAVGPVTKSGT"
BASE COUNT          472 a          609 c          729 g          430 t
ORIGIN      
        1 ggcatgaaaa agagtgggtg gcagatgttc atggcgtctc tggtcggcgc gtggtgtgcg
       61 ttggcggggg agccctacta tcagacggct ttcgaaacgg cggaggaaat ccgcggctgg
      121 aaacgggatg ccaactggaa gatcgaggag ggcgcgggca cgggcggctc gcgggcgctt
      181 gtctggacca acgacgatcc aaagaactac aatgtgaaca gtttcaccat ccccggtggg
      241 cggccgggct tatacttcgt cgcaacgttc aaggtgaaac cggaaaagat cacgggggcg
      301 cggctggggt gcacgatttg ctggacgggc aaggacaagt ggctgggcgg cgcgggcggc
      361 acggtgatga aactcggaga caagcggctc aagccggacg ccgacgggtg gtacgagatg
      421 acgattcgaa tgccgtccga acgtctgccc gaggaagcga cggctgtcca gcttcagctt
      481 tatgtccatg ccggcggaac cggtcgcgtc gctttcgacg acattcgcgt ggacacgttt
      541 gcggatccga agaaagctgc cgtcaagaca ctgacaacgt cctgttatcg cgatatggct
      601 gccacgggga tcgtacacgt ccgtgccggt cttgagttgc ctgacaaata cttgggggag
      661 aaaggactgg tgacggaact tgcatacagg gcttccgatg gtgcacggcg tcaggatgca
      721 atgcgcgcgg tggaagccaa acagttcgtg gcatcaatcc ccgtcacatc gttcgccatg
      781 gggcaacatc cgttggtggc gactctgaag ctggaaaatg gagaggtgtt gggcgagaaa
      841 acccttgtgt tcgaacgtgt tgacacgttg ccgccgcggc gtgtctgctt cgatcgttac
      901 ggtcgcacac tcgtcgaggg gaaacgcttc ttccctctgg gcatgtattg gagcgagaac
      961 acgctggcga agtccaactc tttggagcgt tacgcttcgg ggccgttcaa ctgcctgcag
     1021 acatatgaaa agcagatgac gcctgagata ttggatcgtt actggcgtca tgggttacgt
     1081 gtcgtggcga gcgtcaaaga catctatgtg cgtcctgtcg gcggtccgct gattggcttt
     1141 acgccgccgg aagtcaagac gcggaccgac gagacaaact acgtgaaacg cgttgttgaa
     1201 cgctgccgca accatcccgc gctgctggcc tggtacacct gcgacgagat tccgcatgaa
     1261 tacgccgatc gggtggaaga ccgttattct cttcttaaac agcttgatcc cgaccatccc
     1321 acgttcatct gcctgaacca tcctgtgacc gcgcgcgtat tcatcgattg tacggatgtc
     1381 gtgggatcgg atccttatcc cataccggga tcggaccgcg tcaacacggt cctgcctccg
     1441 gacgccggtg ccgtatggcg cgcgggcgac tcggcgcgga ccatccgaga gagcgtgttt
     1501 ggcctgcgtc cgatgtggca ggttccgcag gcctttgctt ggaagtggga caaaagcgaa
     1561 caacctgaac tgcgtttccc tacccggcgc gaactctcca gcatgacttg gcaacaaatc
     1621 gccgaagggg cgaacgggct tttgttctat tcctacggac agatgatgaa taaagccggg
     1681 gcggaattgg aggaaaactt ccgtacgaca tgttcggtcg cggtcgaggt gcgcgatctc
     1741 atccccattc ttctgttgga gcctggcccg gatgtgcgta aaatgccgcc gcggacgcgt
     1801 gtgcgcacat ggaaagacgg aaagaaagtc tacgtgctcg tctgtaacac gcaccctgat
     1861 cgacgcgtgg gacaggtgga aattgaaggg gcctatacga cgtgcgcacc agtgttcggc
     1921 agcggcgtga caagggcggg cggcgtgttg tcgctcgaca tggactccat gggaacggcg
     1981 gttgtcaggt tggaaccgtg acgcgggtca taaaagcgtt atcccgcaac aaactggtcg
     2041 ggcgcgagaa gcaggtcttc gcggagtgca accgcaaact ggaggaggca cggaagcacc
     2101 gggcgcaagc tcgacaatcc gcaaccggag ccaccccctg cacctgccgc ttcaagatgt
     2161 tgacgcgccc cgccgatcag gatcacgtgg acaacgcgat ccgcgcggtg gctgtcggac
     2221 cggtcaccaa gagcggcacc
//
LOCUS       DAOSRB010000320         2207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_45072_length_2207_cov_4.670074, whole genome shotgun sequence.
ACCESSION   DAOSRB010000320 DAOSRB010000000
VERSION     DAOSRB010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45072_length_2207_cov_4.670074"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..363
                     /locus_tag="PLZ74_11275"
                     /pseudo
     CDS             1..363
                     /locus_tag="PLZ74_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            347..1747
                     /locus_tag="PLZ74_11280"
     CDS             347..1747
                     /locus_tag="PLZ74_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOR98941.1"
                     /translation="MTSSTAWVCLPDRYDDGGFSGGNIERPALKRLLNDVQDGRVDCV
                     LTYKVDRLSRSLMDFSRIMEILDRHEVSFVSVTQQFNTTSSMGRLTLNILLSFAQFER
                     EIIAERTQDKMSAARRKGKWVGGCPVLGYDIAPGGGKLLVNPEEAARVRGIFGLYLRE
                     ESLLDTAREVEARGWTLKLWTNQKGRAKGGKPFNKNSIFKLLTNMIYIGKIDYKGNIY
                     EGEHEPIIDDVTWQGVQTILRRNGQTGGTRVRNKYGALLKGIIRCVPCNAAMVHALTA
                     KKGCRKYRYYVCSSAQKRGWDTCPTKSIPAMEAEQFVVDRIRAIGKDPNLLTTVVTQG
                     RKLHEAKRNELQIEIRQTQERLKRDIEALEETEEHSLERVELRDQIRAAEQRFSILKK
                     ELDDIGSALITDQEARTLLADFDQVWEALTPKECVRVVQLLIEEIGYDGQSGSLSITF
                     RPNGINALRLLKQETS"
     gene            1850..>2207
                     /locus_tag="PLZ74_11285"
     CDS             1850..>2207
                     /locus_tag="PLZ74_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98942.1"
                     /translation="MTCRAYLASAASQFTFWGANKYSCEPLRAQGPSSLAVTDVAGIE
                     WARLTEVKCEQEQKQRKVQFSRRSSDLFEGATCWREWFPDDARIVRASFKVKFRHART
                     PQSISLCPPNVASYSQD"
BASE COUNT          525 a          632 c          646 g          404 t
ORIGIN      
        1 tggcacacgc agggctcggg caagagcctg acgatggcgt tctacgccgg gcgggtggtg
       61 ctgcacccgg cgatgaagaa cccgacgctg gtagtgatca ccgaccgaaa tgacttggac
      121 gaccagcttt acggcacgtt cgcgcgctgt cacgaattgc ttcgccagca gcccatgcag
      181 gctcagagca gggcgaacct gcgggaattg ctgacgaccg cttccggcgg agtcgttttc
      241 acaacggtcc acaagttttt tccgtccgat agtgaggatc aacacccgct gctatcggac
      301 cgccgtaaca tcgtcgtgat cgctgacgaa gcgcatcgca gccaatatga cttcatcgac
      361 ggcttgggtc tgccttcctg accggtatga cgacggcggg ttctccggcg gcaatatcga
      421 gagaccggcg ctcaagcggc tgctcaatga tgtgcaagat ggccgtgtcg actgtgtgct
      481 gacctacaag gtggatcgcc tcagccggtc actcatggat ttctctcgta tcatggaaat
      541 cctcgaccgg cacgaggtct cgttcgtgag cgtaactcag cagttcaaca cgacctcatc
      601 catggggcgt ctgactctta acattctgct gtcgttcgcc cagttcgaac gcgagattat
      661 cgctgaacgt acccaagaca agatgtcggc ggcacgccgt aaaggcaagt gggtcggcgg
      721 gtgtcctgtt ctcggctatg atatcgcgcc tggtggcggc aagctcctcg tcaatccgga
      781 agaggcggcg cgagtccgcg gcatctttgg tctgtacctc cgcgaggaat ctctgctgga
      841 tacagcgcgg gaagttgagg cgcggggctg gacccttaaa ctgtggacga atcagaaagg
      901 ccgggccaaa ggaggcaagc cattcaacaa gaacagcatt ttcaaactcc tgacgaacat
      961 gatctacatc ggaaagattg actacaaggg caatatttac gagggcgaac acgaacccat
     1021 aatcgacgac gtgacttggc agggcgttca aaccatcctt cggcgcaatg gccagacggg
     1081 cggaacgcgg gtacgaaaca agtacggcgc cctgctcaaa ggaatcatcc gctgcgtacc
     1141 ctgcaatgca gccatggtcc acgcacttac cgcgaagaag ggatgtagaa agtaccgcta
     1201 ctacgtctgc tcaagcgccc agaagcgtgg ttgggacaca tgccccacca aatcaatccc
     1261 ggccatggag gccgagcaat tcgtagtgga ccgcatacgg gcaatcggaa aggatcctaa
     1321 cctgctcacg acggtcgtta cgcagggcag gaaactccac gaggccaaac ggaacgagct
     1381 tcagatcgaa atcaggcaga cccaagagcg gctcaagcgc gatattgagg cgctagaaga
     1441 gaccgaggag cactcgcttg agcgcgtgga actgcgcgac caaatccggg ccgctgaaca
     1501 gcggttcagc atactcaaga aggaactcga tgacattggc tctgcgctca tcacggacca
     1561 ggaggcgagg acactattag ccgacttcga ccaagtatgg gaagccctga cccccaagga
     1621 atgtgtccgg gttgtccagc ttctcattga ggaaatcgga tacgacggcc agagcggctc
     1681 attatccatc acgttccgtc cgaacggaat caacgcactt cggctgctca aacaggagac
     1741 atcatgaggt cagtagaaga tgaaaaggtc accatacaat gcagcgtcca cttcaagaac
     1801 aaggcccacg gcagaaaaac attggtccaa ggacctgccc cgaacttagt tgacatgtcg
     1861 cgcatacctc gcgtcagccg cctcacagtt cacgttttgg ggagcgaaca aatactcctg
     1921 cgaaccgctg cgtgcccaag gtccctcgtc gctcgcggtc accgatgtgg ccggaatcga
     1981 gtgggcgcgc ctgaccgagg tgaaatgcga gcaggaacag aagcagcgca aggtccagtt
     2041 cagccgcagg tcatcggacc tgttcgaggg cgccacatgt tggcgggaat ggtttccgga
     2101 cgacgcccgg attgtgcggg cgagtttcaa ggtgaaattc cggcacgcgc ggacgcctca
     2161 aagcatttcc ctgtgtccgc cgaacgtcgc ctcgtactcg caggacg
//
LOCUS       DAOSRB010000321         2204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_45150_length_2204_cov_7.813867, whole genome shotgun sequence.
ACCESSION   DAOSRB010000321 DAOSRB010000000
VERSION     DAOSRB010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45150_length_2204_cov_7.813867"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PLZ74_11290"
     CDS             <1..1219
                     /locus_tag="PLZ74_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR98943.1"
                     /translation="IRHGDTRRLLLRDLLRLQTLMKIHNVFILGLAVAVSLTLKAASP
                     VDLSPIKELLSIPSVSADVAANDKAIDWMRGYLESRGVWCVVETFPKDGRKILYAATK
                     PGLKAPDYVLVTHLDVVAPASPAQFVPVEREGHLYARGACDTKVNAFCGAEALIRLNG
                     RASVGCVFASDEEIGGNTTKHMVSLGYGHPQKAVVVLDAGSMSPDIRYACKGNAYYKV
                     TATGRSGHSARPEACDNPIYKLAEAAMKIRDAYPHQKPGEYGNVASVTIIGGGDSQNR
                     IPETASMTVNVRFVSGGLEDQRALVERITGLKTELIRGTDAAISRPDDPEILRMQRLM
                     KAAYPERACDIVRGLGANDSRYFPQFGKPMMTVSMDHDGGHTDNEWCSVRDIPRFIGL
                     LMAYLAPPDVIGK"
     gene            complement(1444..>2204)
                     /locus_tag="PLZ74_11295"
     CDS             complement(1444..>2204)
                     /locus_tag="PLZ74_11295"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOR98944.1"
                     /translation="LSDIRSAGGKALVRDLQKNMPVLRSAFMSLIPPFTDNTDVSVVR
                     IYQESEAYKQYVGQDNEWSVGFWIPTRRELVILAQDKEREQTMRVITHESFHQYLFYA
                     CNMIPNAVWYNEGHACFFEAAQVGSRGRITISENSRANHLGRNLDAAAALIPKLLKYE
                     YNDFYKSSKEQRTLNYTTAWALIYFLRRGVPEEKLDAYAGILDAYLNSLAATKNAATA
                     TETAFENIDMTKFQKAFTDFWRRNSIRSFEIAAE"
BASE COUNT          460 a          592 c          637 g          515 t
ORIGIN      
        1 cattcggcac ggcgatactc gtcgtctcct tctgcgtgat ctccttcgcc tccaaacctt
       61 gatgaaaatt cacaacgtct ttattttggg gctggccgtc gccgtttcgt tgacgctgaa
      121 ggcagcttct cctgttgacc tctctcctat caaggaactg ttgtctattc catccgtatc
      181 cgccgatgtc gctgcaaacg ataaggcgat agattggatg agggggtatc tcgaaagccg
      241 tggcgtctgg tgcgttgtcg agacgttccc gaaggatggg cgcaagatcc tgtacgccgc
      301 aacaaaaccc ggtctgaagg ccccagacta tgttctcgtc acccatcttg acgtcgttgc
      361 gccggcatcg ccggcgcaat tcgtacctgt ggagcgcgag gggcatctct atgcgcgtgg
      421 cgcctgtgat acaaaagtca acgccttttg cggtgccgag gcgctgattc gactgaacgg
      481 acgcgcgtcc gtcggctgcg tgttcgcgtc agacgaggaa atcggcggta atacgaccaa
      541 gcacatggtt tcgcttggtt acggacatcc ccaaaaagca gtcgtcgtcc tggatgcagg
      601 ttcgatgagt ccggacatac ggtacgcctg taaagggaac gcctattaca aggtcacggc
      661 gactgggagg tcggggcatt cggccagacc ggaggcgtgt gataatccga tttataagtt
      721 ggccgaggcc gcgatgaaaa tccgtgacgc ctatccgcac cagaagccgg gtgagtatgg
      781 caacgtcgcc tcggtcacga tcatcggagg cggcgattcg cagaaccgca ttcccgaaac
      841 ggcgtcaatg accgtcaacg tgcggttcgt gtccggcggg ctagaagatc agcgggcgct
      901 tgtcgagcgc ataacggggc tgaagacgga gttgattcgc gggacggatg ccgctatcag
      961 ccgaccggac gatccggaaa tcctgcgcat gcagcgcctt atgaaggcgg cctatcccga
     1021 acgtgcgtgc gacatcgtca ggggtctggg tgcgaacgat tcgcgctact tcccgcagtt
     1081 cgggaagccg atgatgaccg tatcaatgga tcacgatggc ggccacaccg acaacgagtg
     1141 gtgcagcgtg cgagacatcc cccgcttcat cggactcttg atggcatatc tggcaccgcc
     1201 agatgtcatc gggaagtaaa ggaagcccct ttgaacctaa aaagagcagt tgaaccttca
     1261 acgctctttg gtttctggtt tcagggtttc tggtttcagg gtttcatcca aatcgggatc
     1321 gggatcgcta tcgggatcga ttgtttaacc acggaataca ctaaacacac ggaaacagag
     1381 ccatgccttt ccgtgtgttc ggtgtgagaa aaacccgaac catgcccctt gcgttctctg
     1441 cggctattct gcggcaatct cgaaactgcg gatcgaattg cgccgccaga aatctgtgaa
     1501 tgccttttga aacttcgtca tgtcgatgtt ttcgaaagct gtttccgtgg cggtggcagc
     1561 gttctttgtg gcggccaaac tgttcaggta tgcgtcgaga ataccggcgt aggcatccag
     1621 tttttcctct ggaacgcctc ggcgcaagaa gtagattaga gcccaagcgg tagtgtagtt
     1681 gagcgtgcgt tgttccttag agcttttgta gaagtcgtta tactcgtatt taagcagttt
     1741 cggtatcaac gccgccgcag catccagatt gcgtcccaga tgattggcgc gcgagttctc
     1801 ggatatcgtg atgcgtcccc ggctgccaac ttgagcggct tcgaagaaac aggcatgccc
     1861 ttcgttgtac cacacagcgt tggggatcat gttgcaggca tagaagaggt attggtggaa
     1921 gctttcgtgc gtgatgacgc gcatggtctg ctcgcgctct ttgtcttgcg caaggatcac
     1981 cagctcgcgc cgtgtcggta tccaaaaccc gacactccac tcattgtctt ggcccacgta
     2041 ctgtttgtac gcttccgact cttgatagat gcgtacgacg ctgacgtcgg tgttgtcggt
     2101 gaacggcggt atcagactca taaaagcgct gcgcagcacc ggcatgttct tttgcagatc
     2161 gcgcaccaga gccttgcccc cggcactgcg tatatccgaa agaa
//
LOCUS       DAOSRB010000322         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_45730_length_2185_cov_13.966667, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000322 DAOSRB010000000
VERSION     DAOSRB010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45730_length_2185_cov_13.966667"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1488)
                     /locus_tag="PLZ74_11300"
     CDS             complement(100..1488)
                     /locus_tag="PLZ74_11300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOR98945.1"
                     /translation="MTDIEITRRRFIQVSSAAAALALTGTGGCGMKGSDRAVKIVVVG
                     GGAAGLGMSARLVRLLKKAQITLIDPAERQFYQPGFTLIGSGVYRNPDKVWRRQSDCI
                     PEGVKWVKQAVTALDPAKNTVAVADGTVYPYDFLVLTPGIQCNWDAIEGLSYKTLGEG
                     NVHSIYDFEGAQKTWKAVQAFVEKGGRGVFADTYTKHKCGGAPKKICLLTEHLSRKRG
                     MRDSLKFDFFTASKALYDVPLFTPRLEQIYKERSVSINTRMRVKGVDTAAKKVHFIRT
                     ETIKREVVDPQTLGAVKAVDEVVTTPVVEEYDFLHLAPPQSAPDFVRQAGLGWTEGSL
                     AAEAWVMVDKGTLVHKKFANIVSLGDVAGIPTSKTSAAVRKQIPVAVRNLIALIEGRE
                     PEAIYDGYAACPIVTDYGHVLMAEFDYDKKPKISFPLSLLDMSRERRIAWFLKVYVLK
                     PLYFYGMIPGYW"
     gene            complement(1551..>2185)
                     /gene="lepB"
                     /locus_tag="PLZ74_11305"
     CDS             complement(1551..>2185)
                     /gene="lepB"
                     /locus_tag="PLZ74_11305"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOR98946.1"
                     /translation="VVAGVPHYVPNDAVETDAYRRPVRLAGGVANGASVKAGEVLWSG
                     AVISGDFVFVNRWKWNFSHPTRGEIMVFSTTGIQGLMQGTHYIKRMTGLPNETLQIKA
                     PNLVINGEVVVQPPRMGQIARREAVWPGQPAYAGFNPSGTRQVYFPGQSLATEQDRVT
                     LKADEYYAMGDNSFNSFDSRYWGPVPERNLLGPATVVYWPFTSPRFGWIR"
BASE COUNT          454 a          701 c          565 g          465 t
ORIGIN      
        1 gtttctgctt ctgcggatga cgaactgacc gttctcgtct catggtctcc cgttccgagt
       61 tcgaagttct gagttctacg ttctgcgttc tgcgttgcct caccagtacc ccggaatcat
      121 tccgtagaaa tagagcggct tgagcacgta gaccttcaga aaccaggcta tccgcctttc
      181 ccgcgacatg tcgagcagcg aaagcgggaa actgattttc ggctttttgt cgtagtcaaa
      241 ctccgccatc agcacatgac cgtagtccgt cacgatcgga caagcggcat agccgtcata
      301 aatcgcctca ggctcacgcc cttcgataag cgcaatcaag ttacggactg caaccggaat
      361 ttgtttgcgc acagcagccg atgttttgct ggtcgggata ccggccacat cgccaaggct
      421 cacaatgttg gcgaatttct tatggaccag cgttcctttg tcgaccatca cccaagcctc
      481 ggcggccaga ctgccttcgg tccagcctaa accggcctga cgcacaaaat ccggcgcgct
      541 ttgtggcggg gcgagatgca ggaaatcgta ctcctccaca acgggggtcg tgacgacctc
      601 atccaccgct ttcaccgcgc cgagggtctg cgggtcgacc acctctcttt taatggtctc
      661 ggttcggatg aaatgcacct ttttggcggc agtatccacg cccttgaccc gcatacgcgt
      721 gtttatgctg acggagcgct ctttatagat ctgctccaga cgcggcgtga aaagcggtac
      781 atcgtaaagc gctttggaag ccgtgaaaaa atcaaacttc aggctgtcac gcatgccgcg
      841 ctttcgcgaa aggtgttcgg tcaacagaca gattttcttg ggcgcgccac cgcatttatg
      901 tttggtgtag gtatcggcaa acacaccgcg cccgcccttt tcaacgaacg cctgcaccgc
      961 cttccacgtt ttctgggccc cttcaaaatc gtagatgctg tgcacgtttc cttcgcccag
     1021 cgtcttgtaa gaaagtccct caatcgcgtc ccagttacac tggatgccgg gcgtgagcac
     1081 tagaaaatcg tacggataca ccgtaccgtc cgcaacggcc accgtgttct tcgccggatc
     1141 aagcgccgtg acggcctgct tgacccactt cacgccttcc ggaatgcagt ctgattgtcg
     1201 gcgccacacc ttatccgggt tacggtacac accggagccg atcagggtga aaccgggttg
     1261 atagaactgg cgctccgccg gatcgatcaa cgtaatctgc gcttttttca gcaaccgcac
     1321 caatcgcgcc gacatgccga gaccggccgc gccaccaccg accaccacga tcttgaccgc
     1381 gcggtcgctg cctttcatcc cacaccctcc cgtacccgtc agcgccagcg ccgcagcggc
     1441 cgaagacacc tgtatgaacc tgcgacgcgt aatctctata tctgtcatct tgactctttc
     1501 actatggggc attggtttcg atttcgacac cggtgcatca acccaaccac tcaacgaatc
     1561 cagccgaagc gcggagaggt gaacggccaa tacacgaccg tggccggccc cagcaggttg
     1621 cgctccggca ccggtcccca ataacggctg tcgaagctgt tgaacgaatt atcgcccatg
     1681 gcgtaatact catccgcctt gagcgtcaca cggtcttgtt ccgtggccag cgactgcccc
     1741 gggaaataga cctgacgcgt gccgctcggg ttgaatcccg cgtaagccgg ctgtcccggc
     1801 cagaccgctt cgcggcgcgc gatctgtccc atgcgcggcg gctgcacgac cacctcgccg
     1861 ttgatcacca aattgggtgc tttgatctga agcgtctcat tgggcaatcc agtcatgcgc
     1921 ttgatgtagt gcgtgccctg catcagcccc tgaataccgg tcgtggaaaa gaccatgatt
     1981 tcgccacgcg tcgggtgcga aaagttccat ttccaacggt taacgaaaac gaaatcgccg
     2041 gagatcaccg cgccggacca cagcacctca ccggctttga cgctggcacc gttggcgaca
     2101 ccgcccgcga gccgcacagg acgacgatag gcatcggtct caacggcgtc attcggcaca
     2161 taatgcggca caccggccac aacca
//
LOCUS       DAOSRB010000323         2169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_46194_length_2169_cov_7.183065, whole genome shotgun sequence.
ACCESSION   DAOSRB010000323 DAOSRB010000000
VERSION     DAOSRB010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46194_length_2169_cov_7.183065"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLZ74_11310"
     CDS             complement(<1..437)
                     /locus_tag="PLZ74_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98947.1"
                     /translation="MIQPREYAFCGLFGAAALLLPTLFHLLHLGHVFMPMYLPLVALA
                     FFVRSGAAALTALLVPLLSGAVTGMPPFMPPIAPVMALELALMALMIGTLRQAVPRMP
                     VWCLLALALAVGRIVNALLLYTAARVLELPAGFVAGISLLSGWP"
     gene            complement(482..>2169)
                     /locus_tag="PLZ74_11315"
     CDS             complement(482..>2169)
                     /locus_tag="PLZ74_11315"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOR98948.1"
                     /translation="RQVPGVSISRYSPVGAYGGAQGGSVYVRGAGTARPGGEVRIYSD
                     GVPRESGVWSHPLMDIVPIDFTERITVAKNPQPQHYAGTFGAVDVETRRRKTEGHEGE
                     VSAAYGRFNTLISSAAAGGKIDLFDYYAGAAYKYSEGARQHGEAELRSAFARTGWELS
                     PHDHLSYIFLGTDNWVRDPGPKGGPKPQRDQFNTETLTHAVRLETQRDRIRGYSLIYY
                     EEGEIRWHKDHLSDGVLTSPAGFSNTDWDNYGFRSSYDILLLDRLTLTGSLDSWSEGG
                     ETWNIRSSDNRRVWGYNGRFFTTAPYLGARYEFDVGHDWTLTPSAGARHYVSSEFDNE
                     TAPCAALTLSREGLSVFVSHARGVHYPGVYIRGVSPSTWRSLEAETLDTTEAGVQMEI
                     SEQATLHATLFHTEVDNRMDSTSAGYFNTGDMKAQGAEVSMHFYPRNDLSFFVGGTYT
                     DPETHPVSRMPEVTFTAGSSYRITHALRWDLDAQYVTTQYAYSMRSINPDLEKLDDYL
                     AVNTRLSLDLRALCPLDGELYVALENLTNRHYEYFPDYPMPGIMWYTGMKVKF"
BASE COUNT          444 a          656 c          697 g          372 t
ORIGIN      
        1 ggccagccgc tgagcagcga gatgccggcc acgaaaccgg ccggcagttc caagacgcgc
       61 gcggcggtgt agagcagtaa cgcattcaca atccgtccga cagccagcgc gagcgcgagc
      121 agacaccaga ccggcatgcg cggcaccgct tggcgcaacg tgccgatcat cagcgccatc
      181 agcgccagtt ccagcgccat caccggagca atgggcggca tgaagggcgg catgccggtg
      241 accgcgccgg agaggagcgg caccaacaga gccgtcagcg cggccgcgcc ggaacgcacg
      301 aagaaggcca acgccaccag cggcagatac atcggcatga agacatgccc cagatgcagc
      361 agatggaaaa gcgtcggcag caacagcgcc gccgcgccga acaggccgca aaaggcatat
      421 tccctaggct gaatcatccg tgtcgtgaca atctggttcc accgaaatcg gtatcgtctt
      481 tttagaactt caccttcatg ccagtgtacc acatgatgcc cggcatcggg tagtccggga
      541 aatattcata atgccggttg gtcagattct caagggccac atacagttca ccgtccaacg
      601 ggcaaagcgc gcgcaagtcc agcgaaagac gcgtgttgac agccagatag tcatcaagct
      661 tttccagatc ggggttgatc gagcgcatgc tgtaggcgta ctgcgtggtg acgtattggg
      721 cgtccagatc ccagcggagc gcatgcgtga tacggtagga actgcccgct gtgaaggtga
      781 cctccggcat acgcgagacc ggatgggtct cgggatcggt gtaggtgccg cccacgaaga
      841 acgagaggtc attgcgcgga tagaaatgca tcgaaacttc cgcgccctgc gccttcatgt
      901 ccccggtatt gaaataaccg gctgaggtcg agtccatgcg gttgtcgacc tcggtgtgga
      961 acagcgtggc atgcagcgtc gcctgttcgc tgatctccat ttggacgcct gcttcggtgg
     1021 tgtccagcgt ctcggcctcc agcgagcgcc aagtcgaggg cgaaactccg cggatataaa
     1081 cgccggggta atggacgccc cgcgcatggg aaacaaagac gctcagcccc tcgcgggaga
     1141 gggtcaacgc ggcgcagggt gcggtttcat tgtcgaactc gctgctaacg tagtggcgcg
     1201 caccggccga aggcgtaagg gtccagtcgt ggcccacgtc gaactcataa cgcgcaccga
     1261 ggtagggggc cgtggtaaag aagcgtccgt tgtaacccca gacccgccga ttatcggatg
     1321 agcggatgtt ccaagtctcg ccgccttcgg accacgagtc cagcgatccg gtgagcgtga
     1381 ggcgatccag caacagaatg tcatacgatg agcggaatcc gtagttgtcc cagtcggtgt
     1441 tggaaaagcc ggccggcgaa gtcaggacgc cgtcagacag atgatccttg tgccagcgga
     1501 tttcaccctc ctcgtagtag atcaaggagt atccgcggat acggtcgcgt tgggtttcga
     1561 ggcgaaccgc gtgggtcaag gtctcggtgt tgaactggtc gcgctgcggc ttcggaccgc
     1621 ctttcggacc gggatcacgc acccagttgt cggtgcccag aaagatatat gagagatggt
     1681 cgtgcggaga gagttcccat ccggtccgtg cgaaggcact ccgcagttcg gcctcgccat
     1741 gctggcgcgc gccttccgag tatttgtagg cggccccggc gtaataatcg aacaggtcga
     1801 tcttgccgcc agccgcggca gaactgatca gcgtgttgaa gcggccgtaa gcggcggaca
     1861 cctcgccctc gtgtccctcg gtcttcctcc tgcgggtctc gacgtccacc gcgccgaagg
     1921 tcccggcgta atgctgcggc tgcgggttct tggcgaccgt gatgcgctcg gtaaaatcaa
     1981 tgggcacgat gtccatcaac gggtgcgacc agacaccgga ttcgcgcggc acgccgtcgc
     2041 tatagatccg gacctcgccg ccgggacgtg ccgtgcccgc tccgcggacg tagaccgaac
     2101 cgccctgtgc gccgccgtag gcgccgaccg ggctgtagcg cgagatcgag acaccgggca
     2161 cttggcgca
//
LOCUS       DAOSRB010000324         2130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_47364_length_2130_cov_5.838072, whole genome shotgun sequence.
ACCESSION   DAOSRB010000324 DAOSRB010000000
VERSION     DAOSRB010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47364_length_2130_cov_5.838072"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1899
                     /locus_tag="PLZ74_11320"
     CDS             208..1899
                     /locus_tag="PLZ74_11320"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HOR98949.1"
                     /translation="MAIPVQEIEAIVEQSGARPLTEAEQATLKAAVDTLARITAELET
                     KETTLARLRRILFGAPTESTANVLGEGEKGETPPADTPTRPSAEGGDEQAAGQTPSDT
                     PSPESGVAEPKKKRPGHGRNGAADYPRAPHIAVPHATLRHGDPCPVAGCTGRVYGQRG
                     EPAVLVRITGVAPLLAEVYELERLRCGLCGTVFTADPPPGVGKAKYDDTAAAMIALLK
                     YGCGLPFHRIERLEQGLAIPMPVGTQWEVVAAAAVPLMPVFDELARQAAQGTVVYNDD
                     TTMRILTLTQEARAEALPAGASADRTGVFTSGVVAETPNGLIALFKTGPCHAGEHLAE
                     VLDLREVTDPPIHMSDASTRNRPGDHSTLAASCIPHARRNYVDVVEAFPEEVAFVLHT
                     LRAVFHTDAQAKQHGLSPAERLLLHQKESGPRMKALQDWMAKQFAEHTVEPHSGLGQA
                     ISYMQNHWEKLTLFLRVPGAPLDNNITERILKKAILHRKNALFYRTLNGARVGDLFMT
                     LIHTAELHKIEPFHYLVSLLRHSAKVALDPAAWMPWNYSEALAHAEAESTEPPPD"
     gene            complement(1896..>2130)
                     /locus_tag="PLZ74_11325"
     CDS             complement(1896..>2130)
                     /locus_tag="PLZ74_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98950.1"
                     /translation="AQIEQVFEFVRDECTLYIVSFRQRCETPVARASAARRLIRGDRH
                     GWRAAPRPCAGKPQAATRGPLPFGTLEIVALPG"
BASE COUNT          396 a          739 c          691 g          304 t
ORIGIN      
        1 ttacggcaga cgggagttcc gcgccgcgga ggttgcgagc agccgcgccg ccgcggtcag
       61 tggccgcaag gacccccgcc atcgctcgaa gcgttcccgg aagcggctgc ggggggactt
      121 cggccagctc gttggcgtac ggccatgagc cggagccggc agcgggcatc ccaccggaag
      181 cggcctgcac acaacccgcc gcagcggatg gccatcccgg tgcaggagat cgaggccatc
      241 gtcgagcaga gcggagccag accgctgacc gaggccgaac aggcgacgct gaaggcagcg
      301 gtggacaccc tcgcgcggat cactgccgaa ctcgagacga aggaaaccac gctggcacgg
      361 ctacggcgga ttctcttcgg cgcgccgacc gagagcacgg ccaatgtgct cggggaaggg
      421 gagaagggcg aaacgccgcc agcggacacg ccgacccgtc catcggcgga aggcggcgac
      481 gagcaagccg ccggacagac tccttcggac accccctccc cggaatccgg ggtagccgag
      541 cccaagaaga agcgccccgg gcacggaagg aacggggcgg ccgattatcc gcgagcgccg
      601 catattgccg taccgcatgc gacgttacgg cacggcgatc cgtgtcccgt cgccgggtgt
      661 accggtcggg tgtatgggca acgaggagag cccgcggtcc tggttcgcat caccggcgtc
      721 gcgccactgc tcgcggaggt ctacgaactc gaacggctgc gttgcggctt gtgtggcacc
      781 gtgtttaccg ccgacccccc gccaggcgtc ggcaaggcaa agtacgacga caccgccgcg
      841 gcgatgatcg cgctcctcaa atacggctgc ggtctgccgt tccaccgcat cgagcggctg
      901 gaacagggtt tggccatccc gatgccggtc ggcacgcaat gggaagtcgt ggccgccgcc
      961 gccgtgcccc tcatgccggt cttcgacgaa ctggcacggc aggcagccca aggaaccgtg
     1021 gtgtacaacg atgatacgac gatgcgcatc ctgaccctga cgcaagaggc tcgcgccgaa
     1081 gcgctgcccg ccggcgcgtc cgccgatcgc accggcgtgt tcacttccgg tgtcgtcgcc
     1141 gagacgccca atggtctgat cgccttgttc aagaccggac cctgtcatgc gggcgagcac
     1201 cttgccgaag tactcgatct gcgggaagtg accgatccgc cgatccacat gtccgatgct
     1261 tcgacgcgca accggccggg cgatcattcc acgctggccg cctcgtgcat tccgcatgcg
     1321 cgacgcaact acgtcgatgt ggtcgaggcc tttccggaag aagtggcctt tgtcctgcac
     1381 acgctgcgcg ccgtcttcca caccgacgcg caggccaagc agcacgggct ctcgcccgcc
     1441 gagcgcctgc tccttcacca gaaggaaagc ggcccacgga tgaaggcgct gcaagactgg
     1501 atggccaagc agttcgcgga gcacaccgtg gagccccact cgggactcgg ccaggcgatc
     1561 tcatacatgc agaaccactg ggagaaactg accctgttcc tgcgcgtgcc gggcgcccca
     1621 ctcgacaaca acatcaccga acgtatactc aaaaaggcca ttctgcatcg gaagaacgcg
     1681 ctcttctatc ggacgttgaa cggcgcgcgc gtcggtgatc tgttcatgac cctgatccat
     1741 accgccgaac tccacaagat cgagccgttc cactacctcg tttccctgct gcgccactcg
     1801 gccaaggtgg cgctcgatcc ggcggcctgg atgccctgga actatagcga agccctcgca
     1861 cacgccgagg cggagagtac cgaacctccg ccggactaac ccggcagcgc aacgatctcc
     1921 aacgttccga acggaagcgg accccgggtt gccgcttggg gcttgcctgc gcacggccgc
     1981 ggggccgcgc gccacccgtg gcggtcaccg cggatcagcc gccgcgcggc cgaggcacgc
     2041 gcgaccgggg tttcgcagcg ttgccgaaag gacacgatgt acagcgtgca ctcgtcgcgg
     2101 acgaactcga agacctgctc gatctgggct
//
LOCUS       DAOSRB010000325         2112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_47930_length_2112_cov_6.789985, whole genome shotgun sequence.
ACCESSION   DAOSRB010000325 DAOSRB010000000
VERSION     DAOSRB010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47930_length_2112_cov_6.789985"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1623
                     /locus_tag="PLZ74_11330"
     CDS             154..1623
                     /locus_tag="PLZ74_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR98951.1"
                     /translation="MRIAIAYPPLPSDKGVPLLSQNRQFQWFSRPTYIYPVVPALAAT
                     QAHRAGHEVAWLDGIASGWSPGEFDERLDAFAPEVVMIETKTPVVRRHWEIIRAMKAR
                     RPDTAVVLVGDHVTALPEESFAQCPVDYVLTGGDYDFLLLNLLAALQAAAGGTPDPAA
                     FEPGIYWREGETIRNSGAFRLDHDLKSLPWIDRDLTQWQLYAEKNGNFRRTPGTYIMS
                     GRDCWHGKCTFCSWTTLYPTYRARDPIDVVDEIGELIERYDVREIMDDTGSLPAGAWL
                     KTFCEEMIARGYHKRVRIDCNMRFGCLDEAAYRLMRKAGFRLVLSGLESANQTTLDRL
                     VKALTVEQIEEGARLASHAGLDVHVTVMFGYPWEGESEIANTVNLAQRLLRKGYAYTL
                     QVTMVVPYPGTPLFRELDQAGLLLTREWDEYDMRRQVMASGVPEKSIKAAVRQVYRAF
                     LHPETIVRRIFSTRDPLADLAFYWRGFLSVAGHLRDFKG"
     gene            1839..>2112
                     /locus_tag="PLZ74_11335"
     CDS             1839..>2112
                     /locus_tag="PLZ74_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOR98952.1"
                     /translation="MVVACSGCRTTTPDELGPEPVHVPEGEPTIRPGLTLRVSVTASG
                     EMAVKEELKEVNANGEILMPLIAAVKCEGLTIVELQEKIKVAYKDYY"
BASE COUNT          428 a          572 c          671 g          441 t
ORIGIN      
        1 tgcttacaaa aaacatgcgg gacagtatag aaaaacacga ggcatcaaga caagcaaagt
       61 ccttgcaaag tccttgcttc ccttttggga ggctctcggc taaactcggt ggcgtgtcgt
      121 tgaggactag agtcttgagt gagttgatat tctatgcgca ttgctatcgc ctatccgccg
      181 ttgccgtctg acaaaggggt gcccctgttg tcacagaacc gccagttcca atggttttcg
      241 cggccgacct atatctaccc ggtggtaccc gcgctggcgg cgacgcaagc gcaccgcgcc
      301 ggccatgagg tagcgtggct ggacgggatc gccagcggtt ggtcgccggg tgaatttgat
      361 gagcggctgg acgcattcgc ccccgaggtg gtgatgatcg aaaccaagac gccggtggtg
      421 cgccgccatt gggagatcat ccgcgccatg aaggcgcgcc gccccgacac ggccgtggtg
      481 ttggtgggcg accacgtcac tgcactaccc gaagagagtt tcgcgcagtg cccggtggac
      541 tacgtactga ccggtggcga ctacgacttc ttattgctca atctacttgc ggcgttgcag
      601 gctgcggccg gcggcacgcc tgacccggcc gccttcgagc ccggcatcta ctggcgcgag
      661 ggcgagacaa ttcggaacag cggcgccttt cggctggacc acgacctcaa gagtctgccg
      721 tggatcgatc gcgacctgac gcaatggcag ctctacgcgg agaagaacgg caacttccgc
      781 cgcacgcccg gaacctacat catgtctggg cgcgactgtt ggcacggcaa gtgtaccttc
      841 tgttcgtgga ccacgttata cccgacctac cgcgcgcgtg accccataga tgtggtcgac
      901 gagatcggcg agttgatcga acgttacgac gtgcgtgaga tcatggacga caccggcagt
      961 ctgccggcag gcgcatggct gaagaccttc tgcgaggaga tgatcgcgcg cggctaccac
     1021 aagcgggtgc gcatcgactg caacatgcgc ttcggttgtc ttgacgaagc cgcctaccgc
     1081 ctgatgcgca aggccgggtt ccggctggtg ctgtccgggc tggagtcggc caaccagacc
     1141 acactcgacc gtctggtcaa ggcattgacc gtagaacaga tcgaagaggg cgcacgtctg
     1201 gcttcgcatg ccggactgga cgtacacgta acggtcatgt tcggttatcc atgggaaggc
     1261 gagagcgaga tcgccaatac cgttaacctc gcacagcgtc tgttgcgcaa gggatacgcc
     1321 tacacactgc aagtcacgat ggtagtcccc taccccggca cgccgctctt ccgcgaactg
     1381 gatcaggccg ggctgctgtt gacgcgggag tgggacgaat atgacatgcg ccgccaggtg
     1441 atggcgtccg gcgtccccga aaaaagcatc aaggccgccg tgcggcaggt ctacagggcg
     1501 ttcctgcacc cggaaacgat cgtgcgacgg attttttcca cgcgtgatcc gttggcggac
     1561 ttggcatttt actggcgagg ctttttgtcg gttgcgggcc atcttcgtga ctttaaaggt
     1621 taatgcggtg ggctaacagt actctaaaga tggcttaacg atgtgctaac gttcgatttt
     1681 tttgattttt cctccccttt tctggttgcc gcaccggggt aatggtgata cactgctatc
     1741 gaattctatg ggagagctgt gccggtcccc gtgttgagga agcgacattt ttgaaaagag
     1801 cgatgcaaaa gttcgtttgg cgttgggttt tgtgtttaat ggtcgtagcg tgtagcgggt
     1861 gtcggacgac aacgccggac gagttggggc cggagccggt tcacgttccc gaaggggaac
     1921 cgacaatacg gccggggctg acgttgcggg tttccgtgac tgcctcggga gagatggcgg
     1981 tgaaagagga gttgaaagag gtcaacgcaa atggagagat cctgatgcca ttgatcgcgg
     2041 cggtgaagtg tgagggactg acgatcgtcg agttgcaaga gaagatcaag gtcgcctaca
     2101 aggattacta ca
//
LOCUS       DAOSRB010000326         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_49603_length_2062_cov_5.234679, whole genome shotgun sequence.
ACCESSION   DAOSRB010000326 DAOSRB010000000
VERSION     DAOSRB010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49603_length_2062_cov_5.234679"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1848)
                     /locus_tag="PLZ74_11340"
     CDS             complement(139..1848)
                     /locus_tag="PLZ74_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOR98953.1"
                     /translation="MIRDRTAWFLTVLLTGVFSVLLIWPVARVVAGGFRFEGSWTLRY
                     ITGVFANPIYTEGLLNSLRIAIGATALACVLAVPLAWLGHTFSFRGQRLLSALLLVPM
                     VLPPFVGAIGLMQLFSPYGTLNALLGCGPVDWFGQARYAGVILLQALAFYPIIYLNVS
                     VALANTDPALDEAAQNLGAGLLTRVRRITLPLIMPGLFAGCTLVFIACFTELGTPLIL
                     NVSRCAAVQVYDELKEMSASPFPFALVTVVLVCSVLLYGLSRRLFGEHALTTPMRATL
                     AHQPRPIRGLQAALVALPFVLVILLALLPHAGVVLTSITVPGAWYRTTLPTAFTAAHY
                     AEALGHDMTLSAIRNSLLFSLLAVLVNLVLGVTAAWVIVRSRIRARRFLDSVVMLPLA
                     VPGLVMAFGFIALSSSLCNAPGVRAFPQVAALLDIRVNPSLFLVIAYAVRRLPFMVRA
                     AVAGLQQTSVELEEAAANLGATPCCTLRRVTLPLIAANLIAGTLLAFAFSMLEVSDSL
                     MLAQKKAFYPITKTIFELFQLVGIGRYLASALGVWAMFFLTVTIAGCSLLLGRKLGAL
                     FRA"
BASE COUNT          484 a          623 c          674 g          281 t
ORIGIN      
        1 gcctgcggga gagtaggacg ccgccgggag tttttttccg gggggccgcg gatcgggacg
       61 atccgcggcc cccccttttt tttgtgatcg actcggaact ttggaagatg aatgaatctt
      121 cacagcgcgg ccgtgcgatc aagcacggaa caacgcgccg agtttccgtc ccagcaggag
      181 actgcacccc gcgatcgtca cggtcaggaa gaacatggcc cacacgccga gcgcggaggc
      241 cagataccgg ccgatcccga ccagctggaa gagttcgaag atggtcttgg tgatcggata
      301 aaaggccttt ttctgcgcca gcatcagact gtcggagacc tccagcatgc tgaaggcgaa
      361 cgccagcagc gtaccggcga tcaggttcgc cgcaatcagc ggaagggtga cgcgccgcaa
      421 cgtgcaacat ggtgtggcac cgagattggc cgcggcctcc tccaactcga cagaggtttg
      481 ctgcaggccc gccactgcgg cccggaccat gaagggtagg cggcgcaccg cataggcgat
      541 caccagaaaa agcgaagggt tgacgcggat gtccagcaac gcggccacct gcgggaaggc
      601 acgcacgccc ggcgcgttac acagtgagga actcaacgcg ataaacccga aggccatgac
      661 cagacccggg accgcgagcg gcaacatgac cacgctgtcc agaaatctcc gcgcgcggat
      721 gcgcgaccgc acaatgaccc aggccgcggt aacgcccaga acaaggttga ccagaaccgc
      781 gaggagggag aagagcaggc tgttgcggat cgcggacagc gtcatgtcat gaccgagcgc
      841 ctccgcgtaa tgggcggcgg tgaaggctgt cgggagggtt gtgcggtacc aggcacccgg
      901 tacagtgatg ctcgtgagca ccacgcccgc atgcggcaga agcgccagca gaatcaccaa
      961 cacgaatggc agagccacca gcgcagcctg aagaccgcgg atcgggcgcg gctgatgcgc
     1021 gagggtcgca cgcatcggcg tggtcaaggc gtgttcgccg aatagacggc ggctcaatcc
     1081 gtaaagcagc acactgcaga cgagcaccac cgtgaccagc gcaaaaggaa aagggctggc
     1141 gctcatctcc ttcagttcgt cgtatacctg cacggccgcg caacgcgaga cgttcaggat
     1201 cagcggggtg ccgagttcgg tgaaacaggc gatgaagacc agcgtgcatc cggcgaacag
     1261 tccgggcatg atcagcggca gcgtgatgcg ccggacgcgg gtgaggagcc cggcgccgag
     1321 attctgcgcc gcctcatcca aggccgggtc tgtgttcgcg agggccacgg agacgttcag
     1381 gtaaatgatc ggatagaacg ccaaagcctg aagcagaatt actccggcat aacgcgcctg
     1441 tccgaaccag tcgaccggtc cgcaccccag cagggcgttc aacgtgccat acgggctgaa
     1501 gagttgcatc agtccgattg cccccacaaa cggtggcaac accatgggga ccagcagaag
     1561 ggcggagagc aaccgctggc cgcggaacga gaaggtgtgc cccagccaag cgagcgggac
     1621 ggccagcaca caggcgagcg ccgtggctcc gatcgcgatc cgcaggctgt tgagcagccc
     1681 ctcggtgtaa atagggttgg cgaaaacccc ggtgatatac cgcaaggtcc atgatccctc
     1741 gaaacggaaa ccgcccgcca cgacacgcgc cacaggccag atcagcagta cgctgaagac
     1801 gccggtcagc aacacggtca ggaaccaggc tgtgcgatcg cggatcatgt atcctccacc
     1861 gttgaagcag gatcggttgc gcgtcgcacc tgtgcacggc cgcaacctcc ctacagacgg
     1921 atacaaagca tgatactgcg ggtacacgcg cgcgcaagcg cgaagtgtga aaaaagagca
     1981 aggggtgttg tccggaaaag gcggaaacgg tatactcaca cacaaaggtc agccacacgc
     2041 gggaagcggg ctgaagcgac gg
//
LOCUS       DAOSRB010000327         2054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_49876_length_2054_cov_9.230615, whole genome shotgun sequence.
ACCESSION   DAOSRB010000327 DAOSRB010000000
VERSION     DAOSRB010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49876_length_2054_cov_9.230615"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..>2054)
                     /locus_tag="PLZ74_11345"
     CDS             complement(102..>2054)
                     /locus_tag="PLZ74_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR98954.1"
                     /translation="PLASFAARGVWLEAEGFEQTGGWLIEQQSFGQVGSAYLMAHGMG
                     IPVDDAITTCTVPRAGKWTVWVRTRDWTAPWKRGTPAGRFQIVVNGTPLPETLGTNGE
                     AWGWQKAGTLPLDKGSAEIALRDLTGFNGRCDALYLTDQPGRVPANDPAALAELRQKT
                     LGTKRKDDPTVYDLAIAGGGISGICTAVAAIRSGAKVILIQDRSVLGGNNSSEIRVPM
                     GGILNKPPYPRLGQVVKEIEPITGRPGPRPPEYFEDARKKHVFETCPQHLYRLVLNTR
                     VFGIEKSESGKISAFLTRNIRTGEETRIRAKFFADCTDDAVIARMAGASVMYGRESRA
                     IFGESLAPIKGDRQVMGMSIVWYCTRTPSPSAFPAIDWGHELDENTVYYVRSGDWEWE
                     TGQYRDQADETEYIRDYGLMTIFANWSYLKNHAARKAEWANERLAWISPLGGKRESYR
                     VVGDMVLTQNDIEQRVIYPDATASATWNLDLHFPDPIHEAKFKEPFRSCAYHRNITKP
                     YPVPFRCLYSKDIENLFLAGRHISVSHVAFASVRVMRTLGALGEVTGMAAALCAKHNI
                     SPRELHATRLDDLKEMMKRGVPPPPTYHPGGFGSPYEGYHFKDTGHLGVYPNPSPRLN
                     EPSVKARIEALGVQHVETEKQRPSGY"
BASE COUNT          385 a          588 c          618 g          463 t
ORIGIN      
        1 agtggcgact cagctcactg acagagagtt ccggccgctc catcagtagc tcaagaatct
       61 gctgtttccg attgcgtttt ttggtcattc agcgagttct ttcaatagcc cgaaggacgt
      121 tgcttctctg tttcaacatg ctgcacgccc agtgcttcaa tacgtgcttt gacgctaggt
      181 tcattcaagc gcgggctggg gttcggatag acgccaagat gtcccgtatc tttgaagtga
      241 taaccttcgt atggcgatcc gaagccgcct ggatgataag tgggtggagg gggcacgcca
      301 cgtttcatca tttccttgag gtcatcaagc cgggtggcgt gaagttctcg gggtgagata
      361 ttgtgtttcg cgcacagagc cgccgccatg ccggtcacct cgcccagtgc gcccagcgtg
      421 cgcatcacgc ggaccgaggc gaatgcaacg tgcgatacgc tgatgtgtcg tccggcgagg
      481 aaaaggttct caatgtcttt tgagtaaagg caccggaatg gaacaggata gggcttggtg
      541 atgtttcggt gataggcgca cgagcggaag ggctccttaa acttggcttc gtgaatgggg
      601 tcggggaagt gcagatccag attccatgtg gccgacgccg tggcgtcggg atagatcacc
      661 cgttgctcaa tgtcattctg cgtcaggacc atgtcgccga ctacacggta gctctcccgt
      721 ttcccgccga gcggcgagat ccaggcgaga cgttcattgg cccactcggc cttgcgcgcg
      781 gcgtggttct tgagatagga ccagttcgcg aaaatggtca tcagtccgta atcgcggatg
      841 tattcggtct catcagcttg gtctcggtat tggccggtct cccattccca gtcgccgctg
      901 cgtacataat agaccgtgtt ctcgtcaagt tcatgccccc agtcgatcgc cgggaaggcg
      961 gatggagatg gcgtgcgtgt gcaataccag acgatcgaca tacccatgac ctgtcggtcg
     1021 ccttttatcg gcgcgaggga ttcgccgaat atcgcgcgtg attcacggcc atacatcacc
     1081 gatgcgcccg ccatgcgcgc gatgacggca tcgtccgtgc agtcggcaaa aaacttggcg
     1141 cggatacgtg tttcctctcc ggtccggatg tttcgcgtga ggaaagccga aatcttgccg
     1201 gattcacttt tttcgatgcc gaagacgcgc gtgttgagca cgagacggta caggtgttgc
     1261 ggacaggtct cgaacacatg ctttttgcgg gcgtcctcga aatattcagg cggacgcgga
     1321 ccggggcgac ccgtgatcgg ttcgatctct ttgacaacct gaccgaggcg cgggtagggg
     1381 ggcttattta gaattcctcc catcggtacg cggatttctg aactgttgtt gccgcccaag
     1441 acggaacgat cttgaatcag gatcactttt gccccagatc gtatcgcggc aacggctgta
     1501 cagatgccgg aaatgccgcc gcccgcgatc gcgagatcat aaacggtcgg atcatctttc
     1561 cttttggtgc ccaaggtctt ttgccgtagc tccgctaacg cggcgggatc gtttgccgga
     1621 accctgccgg gctggtcggt caggtagagg gcgtcgcaac ggccgttaaa accggtcagg
     1681 tcacgcagcg cgatttcagc ggaacctttg tcaagcggga gcgtgccagc cttctgccag
     1741 ccccacgctt cgccgttcgt gcccagcgtt tccggcagcg gtgtcccgtt caccacgatc
     1801 tgaaaacggc cggcgggcgt gccgcgtttc cacggtgcgg tccagtcgcg cgtacgcacc
     1861 cagaccgtcc atttgcccgc acgcgggact gtgcaagtcg ttatggcgtc gtcaaccggg
     1921 attcccatgc cgtgcgccat aaggtaggcc gagccgacct gtccgaatga ctgctgttca
     1981 atcagccatc cgccggtctg ctcgaaccct tcggcttcga gccagactcc ccgcgcggcg
     2041 aaactcgcca aagg
//
LOCUS       DAOSRB010000328         2050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_50026_length_2050_cov_8.524812, whole genome shotgun sequence.
ACCESSION   DAOSRB010000328 DAOSRB010000000
VERSION     DAOSRB010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50026_length_2050_cov_8.524812"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1119
                     /locus_tag="PLZ74_11350"
     CDS             265..1119
                     /locus_tag="PLZ74_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98955.1"
                     /translation="MRWQERVAGRRILTAAVLCLAGAASGTAERYVPKPFEYYQPILD
                     RMPFGAAPAQTGAGAQDAESAKSAAQEKIEQERLAKQVNMSAVNITPEGRTAIGFTDL
                     SQKPPVNHYLLVGDEADGWKVLEADYDLETATIEKEGVAITLKLGQGLQPPAAAAAGA
                     PAAGARKAGEVPVASVLPPGLTRRVRRPVAGTVAGAGGGPASGTAPVTKPPPSSYVER
                     NRERALQREKEEQASREKMQAQLAELARKAALNEMRRRDQEEAEARAAEQAEEAAAAQ
                     QEEEQSAL"
     gene            1140..1925
                     /locus_tag="PLZ74_11355"
     CDS             1140..1925
                     /locus_tag="PLZ74_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOR98956.1"
                     /translation="MDAFSRTALLLGQPAIKRLHASRVAVFGLGGVGSYVVEALARAG
                     VGRFLLVDHDRISVSNLNRQILALHSTLGRLKTEVAAERVRDINPRAEVETRALFYLP
                     ETADAIDLAACDYVVDAVDTVSAKVTLAVCAAEAGVPIVSCMGAGNKLDPMRFEVADL
                     FATSVCPLCKVMRREMKARGIRALRVVYSREPPLLPASGEDAAYAECRRPTGSVPSVP
                     AAAGLLLAAEVIRSLTDAGDLERMKRKGRACAPGESPQHTEGI"
BASE COUNT          404 a          581 c          704 g          361 t
ORIGIN      
        1 gccgcgcggc tccgccgcga aaaaaccttg gaataagttt aggacatgat taacacgatg
       61 ttgttcaatc ctgaaaatcc tgtaatcctg gcaatttagc agctttaggc aaggagtggc
      121 gcacgcagac gaaaacgcga acagaaatct tgttcattcc ggcttgcctt gatgcgccca
      181 gagcggcaca cgcggtggaa ttcctctttc acgactgtgg tccggtctgc tatactttct
      241 gacatgaacg gtacggggaa tcatgtgcgg tggcaagagc gggtcgcggg aaggcggatc
      301 ctcacggcgg ctgtcctgtg tctggcaggt gccgcttccg gcacggccga acggtatgtg
      361 cccaaaccgt tcgaatatta ccagccgatt ctcgaccgca tgccgttcgg ggctgcaccc
      421 gcgcaaacag gtgccggagc gcaggatgcc gagagcgcga agagcgcggc gcaggagaag
      481 atcgagcagg agagactcgc caagcaggtc aacatgtcgg cggtgaacat cacacccgaa
      541 gggcgcaccg cgatcggatt caccgatctc tcgcagaagc cgccggtcaa ccattatctg
      601 ttggtcggcg acgaggcgga cggctggaag gtgctcgaag ccgattatga tctggagacc
      661 gcgaccatcg agaaggaggg tgtcgccatc acgctgaaac tcggacaggg gctacagccc
      721 cctgccgctg cggctgcagg cgcacccgca gcgggagcca ggaaagcggg ggaagttccg
      781 gtagcgagcg ttctccctcc ggggctcact cgccgagtgc ggcggccggt ggctggaaca
      841 gtggccggcg ctggtggcgg accggccagc ggcaccgcgc cagtgacgaa gccccctccc
      901 tcctcgtatg tcgagcggaa ccgcgagcgt gccctgcaac gtgagaagga ggagcaggcg
      961 tcgcgagaga agatgcaggc acagctcgcc gagttggcgc gaaaggccgc gcttaatgag
     1021 atgcggcgcc gcgatcagga ggaggcggag gcgcgggccg ccgaacaggc ggaggaggcc
     1081 gcagccgcgc agcaggaaga ggagcagtcg gccctgtgat tccggatggg acgccgcgca
     1141 tggacgcttt ctcacgaacg gccctgctgt tggggcaacc agccataaaa cggcttcacg
     1201 ccagccgcgt ggcggtcttc ggtttgggcg gggtgggttc gtatgtggtc gaggcgctgg
     1261 cccgcgccgg ggtcggacgc ttcctgctgg tggatcatga ccggatcagc gtttccaatc
     1321 tcaaccgcca gattctcgcc ctgcacagta cgcttgggag gctgaagacg gaggtggcgg
     1381 ccgagcgtgt gcgggatatc aacccgcggg cggaggtcga gacgcgtgcg ctgttctatt
     1441 tgccggaaac cgccgatgcc attgatctcg cggcctgcga ttacgtggtg gacgcggtcg
     1501 acacggtaag cgccaaggtg acgctcgccg tgtgcgcggc agaggcaggc gtgccgatcg
     1561 tgagttgcat gggtgccggt aacaaactcg acccgatgcg ctttgaggtg gctgatctgt
     1621 tcgcgacctc ggtctgcccg ctctgcaagg tgatgcgcag agagatgaag gcgcgcggga
     1681 tacgcgcgct acgcgtggtg tactcccgcg aaccgccgct tcttccggct agcggggagg
     1741 atgccgcgta tgcggagtgt cgccgtccaa ccggcagcgt cccctctgtg cctgccgcgg
     1801 cgggattgct gctcgccgca gaggtgattc gttccctgac cgacgcgggg gatctggaac
     1861 ggatgaaaag aaaggggagg gcatgcgctc ccggcgaatc cccgcagcac acggaaggta
     1921 tctgatgcga catgatccgg ctccaggcgt ttttcttctc aaataataac gaagggacag
     1981 gtgtttagat ttgaataagt ttaggacatg attaacatga tgttgttcaa tcctgaaaat
     2041 cctgtcatcc
//
LOCUS       DAOSRB010000329         2033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_50635_length_2033_cov_3.262892, whole genome shotgun sequence.
ACCESSION   DAOSRB010000329 DAOSRB010000000
VERSION     DAOSRB010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50635_length_2033_cov_3.262892"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLZ74_11360"
     CDS             complement(<1..215)
                     /locus_tag="PLZ74_11360"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOR98957.1"
                     /translation="MHSILFHIGPIPIRAFGLCMALGFLSGWQAAVWLCRRTGQNPDR
                     LTALLTWLMLSAVLGARTAYVLEHWTT"
     gene            442..1122
                     /gene="ispD"
                     /locus_tag="PLZ74_11365"
     CDS             442..1122
                     /gene="ispD"
                     /locus_tag="PLZ74_11365"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOR98958.1"
                     /translation="MNTAILVAAGKSERMGSNTDKAFLNLGSKPVVAWSLLAFEECTT
                     IDQIILVVRKDQILAAKSVAQMFGISKIRAIVAGGARRQDSVQNGMKAMDPDTRVGVV
                     HDGARPCVTPELISEVIKCAKRNGCGVAASRVWDTIKYVERGTTVDHTVDRTKLWAVQ
                     TPQAFNVELLKRAYKTVEEQKATVTDEAGAVELIGEPVRLIEWALPNIKITTAEDLPL
                     AAAAMKIT"
     gene            1140..1871
                     /locus_tag="PLZ74_11370"
     CDS             1140..1871
                     /locus_tag="PLZ74_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOR98959.1"
                     /translation="MMDRKYAIFGDIHANFEALTAVVEDARSKGVTDFVCVGDVVGYN
                     ASPSQCIKVIRDLGCVTVRGNHDHYCSYNESLDDFQPLAASVIAWTRRQLTEEDGKWL
                     RELPYQKACGGFTLVHSTLDMPERWGYVFDTLAAEANFSYQVTSLCFHGHTHVPMIYE
                     KQREIMRIEPCKIKIAFGQKFFINVGSVGQPRDSDPRSAYVLYSAKSKELEFVRVRYD
                     VDAAMEKNMKAGLPERLALRLAQGH"
BASE COUNT          441 a          497 c          650 g          445 t
ORIGIN      
        1 tccgttgtcc agtgctccag cacatacgca gtgcgggcgc ctaatacggc ggaaagcatc
       61 agccaggtca gcagcgcggt gaggcggtcg gggttctgcc ccgtccgcct gcaaagccat
      121 acggcggcct gccaaccgga caggaaccct agcgccatgc aaagaccaaa cgcgcggata
      181 gggattggcc cgatgtggaa aagaatcgaa tgcatgacgg gtatcatagg agttcgcctg
      241 tctgtccgca actcaaatga ggcaaaccgg tagatacttg acgtttgatt ttcccgttgg
      301 tactattcaa acattccttg cagaattttg atttatgtgc agcggccgac ggaacatccg
      361 cgggagtaag ggtcgggtga ccgggctgtg ttgggcgccg tctgggcata aaaactgctt
      421 cgatattttt tggagagacc tatgaatact gccattcttg ttgcggccgg aaaaagcgag
      481 cgtatgggct cgaatacaga caaggctttt ctgaacctgg ggtctaagcc tgttgtcgcc
      541 tggtcgctgt tggcgttcga agagtgtacc actatcgacc agatcatcct ggtcgtgcgt
      601 aaagatcaga ttctggccgc gaaaagtgtg gctcagatgt tcggcatatc caagatccgc
      661 gcgatcgtgg ccggtggcgc gcggcgtcag gactccgtcc agaacgggat gaaagcgatg
      721 gatccggaca cgcgtgtggg ggtcgtccat gacggcgcgc gcccgtgcgt gacgccagag
      781 ctgatttcag aggtcatcaa atgcgcgaag cgtaacggat gcggcgtggc ggcatcgcgc
      841 gtgtgggaca cgatcaaata tgtggagcgc gggacaacgg tggatcacac ggtggaccgg
      901 acaaaactct gggcggtgca gacgccccag gccttcaatg tggagctgtt gaagcgcgcg
      961 tacaaaacgg ttgaggagca gaaggccacg gtgacggacg aggcgggcgc ggtggaactg
     1021 atcggcgagc cagtccgcct gatcgagtgg gctctcccca acatcaagat aacgacggcg
     1081 gaagacttgc cgttggccgc cgccgcgatg aaaatcacgt aatcactccg tttttattta
     1141 tgatggatag gaaatatgcc atatttgggg acatccacgc gaactttgag gcactgaccg
     1201 cggttgttga ggatgcccgc agcaaggggg tgaccgactt tgtctgcgtg ggggatgtcg
     1261 tggggtacaa tgccagcccg tcccagtgca tcaaggtgat ccgtgatctc gggtgcgtga
     1321 cggtgcgcgg caaccacgat cactattgtt cctataacga gagcctcgac gatttccagc
     1381 cgttggccgc gagtgtgatt gcctggacga ggcggcagtt aacggaagag gacggcaagt
     1441 ggttgcgcga attgccttat cagaaagcct gtggcggatt cacattggtt cacagtacgc
     1501 tcgacatgcc ggaacgctgg ggatatgtgt tcgacacgct ggcggccgag gcgaacttca
     1561 gttatcaggt gacgtcgctc tgctttcacg gtcacacgca tgtcccgatg atctatgaga
     1621 agcagcgcga aatcatgcgg attgaaccgt gtaagatcaa aatcgctttt ggacagaagt
     1681 tttttatcaa cgtggggagt gtggggcagc cgcgggacag tgatccgcgc agtgcctatg
     1741 tgctgtattc cgccaagtct aaagaactgg aatttgtccg ggtgcggtac gacgtcgatg
     1801 ccgcgatgga aaagaatatg aaagccgggc ttcccgaacg gctggccttg cgtctggcgc
     1861 aggggcatta aggtgccgtg tcccgtttcc gggccattca atttgggctg gatttctccg
     1921 tccggggaag gtaatttatt tcttatcgca tggggtaaca gggagcataa aaatgggtag
     1981 gtttctcgca ctagcgtttc ctgtgacggc tttttttctg ctgatgtttg ggc
//
LOCUS       DAOSRB010000330         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_50700_length_2031_cov_6.398785, whole genome shotgun sequence.
ACCESSION   DAOSRB010000330 DAOSRB010000000
VERSION     DAOSRB010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50700_length_2031_cov_6.398785"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1385
                     /locus_tag="PLZ74_11375"
     CDS             402..1385
                     /locus_tag="PLZ74_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98960.1"
                     /translation="MGNYWIGQCGVNVDVGMEKNSTEFFIDHKIWYTNAPSAKKKVTR
                     IQDKEHDKDKEGDTIFFRNEGRIKASGIVEKKGNYSPHPIFFLEKVEPTDVPSPVEFP
                     ECLQTLIGPLSKDHKHVRAILDAMAEAKKPSIVNGTDRQKGQCAMNETPGAKPNAKTL
                     LALSGLPNAGKTQTIKKVYRLLRETYPEAKVTWVDPEEEPAEPFDRDICLFMEIGGTL
                     IGIESPGDDSSHLQKSLKRFAEHQPPCDVILCARRTGKGTRGMAGVIEHLHTVYGYDI
                     VPIPKKKPHRKNTKCTDEESVKQRIDRENNECAKHILTKIVRLLAGKEARP"
     gene            1382..>2031
                     /locus_tag="PLZ74_11380"
     CDS             1382..>2031
                     /locus_tag="PLZ74_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOR98961.1"
                     /translation="MKTTPSRMFRALCASAAFWLAASPAVAGTWTYNISNNRITHSGT
                     AWVLNVSRSGTSLTVTGVYTAPSVASALPLDDAVSGYQITSIGSSAFSGRTGLTGVTI
                     PGSVTNIGSYAFAGCSKLTSVEIGSGVTSIGSSAFYNCSAIRDVTLNANLPSFRASSV
                     FSSGYWSWTNLVIGGTVTNIGSSAFSGCSGLTGVTIPDGVTSIGDSAFSGCTGLTSV"
BASE COUNT          481 a          548 c          624 g          378 t
ORIGIN      
        1 ctttccagtg tggcgtgtgc cactagttac cgagcgaagc gaggttactc ttgaaaaagg
       61 aagaccgcat gaccgacgga acaggatttt ttaaaccgtt gatgcccgcg cctcacattg
      121 gcacgggact tgcgtctgtc tatgctgatt tcatagcttt tgattttgca aacgcaaggc
      181 cgcgtcccgc cgttcaggcg gggcgcggcc tttttgtttg cgcggaacgg ggcgcccggc
      241 aaggcggcgc gcggggggcg cgttgcatcg ctccggtttt gcaggatttc acggaaaatg
      301 tggttcgggg tgcgccttcc ttgtgccagt gggagacgcg gccccgcaac gggtgacagg
      361 catgggcaga ggaaagccgg caggctgaaa gagaggcaaa aatgggaaac tactggatcg
      421 ggcaatgcgg ggtgaatgtg gatgttggga tggagaagaa ttcgacagag ttctttatcg
      481 atcacaaaat ctggtatacg aacgcacctt cagcaaaaaa gaaggtgacg agaatccaag
      541 acaaagagca cgacaaagac aaagagggcg acaccatctt ttttcgcaac gaaggccgca
      601 tcaaagcgag cggcattgtt gagaaaaaag ggaactattc gccgcatccc atcttttttc
      661 tggaaaaagt cgagccgaca gatgtgccgt cgccggttga attcccggaa tgcctgcaga
      721 ccttgatcgg gccgctctcc aaagaccaca agcacgtccg cgccatcttg gacgccatgg
      781 cggaggcgaa gaaaccatcg atcgtgaacg ggacggacag acagaaagga cagtgcgcga
      841 tgaacgaaac accgggcgca aaaccgaatg caaaaacact tcttgcgttg tctggattac
      901 cgaacgcggg gaagacgcag acgatcaaga aggtgtatcg gctgctgcgg gaaacgtatc
      961 cagaggcaaa ggtcacatgg gtcgatccgg aggaagaacc cgccgaaccc ttcgacagag
     1021 acatctgcct gttcatggaa atcggcggga cgttgatcgg gatagaaagc ccaggcgacg
     1081 acagcagcca cctgcagaaa agcctgaagc ggttcgcgga acatcagccg ccttgcgatg
     1141 tgatcctgtg cgcccggcgg acaggaaagg ggacgcgcgg aatggctggg gtgatcgagc
     1201 acctgcacac tgtctatggc tatgacattg tcccgatacc caagaagaaa cctcaccgga
     1261 agaacacgaa gtgtacggat gaagagagcg tcaagcagcg catagaccgg gagaacaatg
     1321 aatgcgcaaa gcacatcctc acgaaaattg tgcgcctgct ggcgggaaag gaggcgcggc
     1381 catgaaaaca acacccagtc gcatgttccg cgcgctctgc gcgtccgccg cgttctggct
     1441 cgccgcttct cccgccgttg ccggaacgtg gacgtacaac atttccaaca accgcatcac
     1501 ccacagcggc accgcatggg tgctcaatgt gtcgcgcagc ggcacaagtc tgaccgtgac
     1561 gggcgtatac accgctccgt ctgtcgcatc cgcccttcct ctggatgacg ctgtcagcgg
     1621 ctatcagatc accagcatcg ggagttccgc gttttcagga cgcacggggt tgacgggcgt
     1681 gacaatcccc ggcagcgtga ccaacatcgg ctcttacgcg tttgctggct gttccaagtt
     1741 gaccagcgtg gagatcggga gtggcgtgac cagcatcggc agctccgcat tttacaattg
     1801 ttctgccatc agggatgtga cgctcaacgc caacctgccg tcattccggg cgtcgtcggt
     1861 gttttcgagc ggttactggt catggacgaa tctggtcatc ggaggaaccg tgacgaatat
     1921 cgggagttcc gcgttctccg gatgcagcgg gctgaccggc gtgacgatcc cggacggcgt
     1981 gaccagcatc ggggattctg cgttttccgg ctgcaccggc ttgaccagcg t
//
LOCUS       DAOSRB010000331         2028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_50796_length_2028_cov_10.512418, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000331 DAOSRB010000000
VERSION     DAOSRB010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50796_length_2028_cov_10.512418"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..170
                     /locus_tag="PLZ74_11385"
     CDS             6..170
                     /locus_tag="PLZ74_11385"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98962.1"
                     /translation="MTDHQILTPDRTVQRTVFANGVTVTVNFGERPYRMSDGNDIPAL
                     DVRVVYPEGQ"
     gene            276..671
                     /locus_tag="PLZ74_11390"
     CDS             276..671
                     /locus_tag="PLZ74_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOR98963.1"
                     /translation="MSKTVLVQARISPEVKADVEKLFEAWGFDISTAFRMFLNKVSRD
                     KAMPFIFAEENQPTAEELHDMRVLGIKKRADLLKVYREIDAARADYEVGRGASFGSVA
                     EMFKDTFGEPYVDSLSHGDVQRATEGSEK"
     gene            613..870
                     /locus_tag="PLZ74_11395"
     CDS             613..870
                     /locus_tag="PLZ74_11395"
                     /inference="COORDINATES: protein motif:HMM:NF018476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOR98964.1"
                     /translation="MLIPYRTETFNAQLKVLRNNQVVVARIFRLIMDACEHPFSGLGK
                     PEPLKYGKAGLWSRRIDKRNRLIYKVIEPDLILLSCIGHYD"
     gene            930..>2028
                     /locus_tag="PLZ74_11400"
     CDS             930..>2028
                     /locus_tag="PLZ74_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98965.1"
                     /translation="MRMMMWVVCCLIAVSVSAETGDGVLVKRVVEGGGHAEKLTPLAP
                     ERAGAYGAGFTRDGVEYICDNGDQAAQQRGVAWSIELNQSRAEPVVASAWARAEQTEG
                     SPNSDFSLYLDLVYTDGTPLWGQSVPFDPDPAVGWQRREVVVTPDRPIRRISYYLLYR
                     NRAGRVRFKDARFGALAIDDVLRFDGAVTEQVGAPQRGFLIRDVARESGFVSITREAL
                     GVRLTTRERRAAGVAFYDVSLEELTGQDRAMTLVYSLPQPTGSLVWFHDPRRAMDLDG
                     RMAEVMNTTRRQVGANGQLSRYPFGAVAAGGKGCALGIDTATPLYYRVGCQPRTRELF
                     LACDIGFAPEKRTADFRFCVFDFKADDGFRGA"
BASE COUNT          380 a          495 c          706 g          447 t
ORIGIN      
        1 acgagatgac cgatcaccag attctgacgc cggaccgcac tgtgcagcgc acggtcttcg
       61 ccaacggggt gacggtcacg gtgaatttcg gtgagcgccc gtaccgtatg tccgacggga
      121 acgatatccc ggcgctggat gtgcgggtcg tgtacccgga gggacagtga gcgtgagcgt
      181 tgacagccgc gcatgattta gtatgataat gacgttgttt gaattggttc tgcaccggac
      241 ttaatgtcgt gtgcggaata ttcgggaggg acgtcatgtc gaaaaccgtt ttagttcagg
      301 cgagaatctc gccggaagtg aaagcagatg tcgagaaact ttttgaggct tggggcttcg
      361 atatctcgac cgcattcagg atgttcctca acaaggttag ccgagacaaa gcgatgccgt
      421 tcattttcgc cgaagaaaat cagcctacgg cggaggagtt gcatgacatg cgtgtcttgg
      481 ggatcaagaa acgcgcggat ctgctcaagg tgtaccgtga gatagacgcg gctcgggccg
      541 attacgaggt gggccgcggg gcttcgttcg gtagcgttgc tgagatgttc aaagacacgt
      601 tcggagagcc atatgttgat tccctatcgc acggagacgt tcaacgcgca actgaaggtt
      661 ctgagaaata atcaagttgt tgtggcgagg atattccgcc tgattatgga tgcctgtgag
      721 catcctttct ctggccttgg aaaacctgag ccgcttaaat acggcaaggc gggattgtgg
      781 tcgcgtcgca tcgacaaaag gaatcgactt atttataaag ttatagaacc tgatctgatt
      841 ttgctttcgt gcatcgggca ttacgattag gggaaggatc cgtacggggt agggcgttac
      901 aacgaggtcg aaggctaacg gaggtagtta tgcgcatgat gatgtgggtt gtgtgttgtc
      961 tgatcgcggt gagtgtgtct gctgaaaccg gagacggtgt gctggtcaag cgggtggtgg
     1021 agggtggagg tcacgctgag aagctgacgc cgctggcacc cgaacgcgcg ggggcgtacg
     1081 gggcggggtt cacacgcgac ggtgtggagt atatctgcga caacggggat caggcggcgc
     1141 agcagcgtgg tgttgcgtgg agcatagagt tgaaccagag ccgggctgag ccggtggtgg
     1201 catcggcttg ggcgcgtgcc gagcagacag agggttcgcc caacagtgac ttctcgctct
     1261 atcttgatct tgtgtatacc gacggcacac cgctctgggg gcagtccgtg ccgttcgatc
     1321 ctgatccggc ggtggggtgg cagcggcgtg aggtggtggt tacgccggac cgcccgattc
     1381 gacgcataag ctattacttg ctttaccgta accgcgccgg gcgggtgcgt ttcaaagatg
     1441 cgcgtttcgg agcgctcgct atcgacgatg ttctgcggtt tgacggtgca gtgaccgagc
     1501 aggtcggtgc gccgcagcgc ggcttcctga tacgtgacgt ggcgcgggag tcgggctttg
     1561 tatcgatcac gcgcgaagcg ctcggcgtcc ggctgacgac ccgcgagcgg cgcgccgccg
     1621 gcgtggcctt ttatgatgtg tcgctggaag aactcaccgg ccaggatcgc gcaatgacgc
     1681 tcgtctactc cctgccgcag ccgaccgggt cgttggtatg gttccatgac ccgcggcggg
     1741 cgatggactt ggacgggcgc atggcagagg tcatgaacac cacgcggcgg caggtcggcg
     1801 cgaacggcca gctttcgcgc tatccgttcg gggcggtggc ggctggcggg aaggggtgcg
     1861 cgcttggtat cgacacggcg acgccgctct actaccgggt cggctgtcag cctcggacgc
     1921 gcgaactgtt tttggcgtgc gatatcggtt tcgcgccgga gaagcggacg gcggatttcc
     1981 gtttctgcgt ttttgatttc aaggcggacg atggttttcg cggtgcgc
//
LOCUS       DAOSRB010000332         2018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_51193_length_2018_cov_10.310749, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000332 DAOSRB010000000
VERSION     DAOSRB010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51193_length_2018_cov_10.310749"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLZ74_11405"
     CDS             complement(<1..227)
                     /locus_tag="PLZ74_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOR98966.1"
                     /translation="MKRIGIIGAGRFGSSLAESLAEHGAEVLLIDQNREIIQELSEFV
                     TRAVEGDVTNRRTLEDAGFQECDVVVVAIGN"
     gene            complement(255..1532)
                     /locus_tag="PLZ74_11410"
     CDS             complement(255..1532)
                     /locus_tag="PLZ74_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOR98967.1"
                     /translation="MSFHPDTLCVQAGWQPKNGEPRVLPVFQSTTFKYDSSEHVGNLF
                     DLKVPGHFYTRLSNPTAEAVENKIAALEGGVGALMTSSGQAASLIAILNLCNSGDHFI
                     ASSAIYGGNTNLFSVTLKKLGIEVTYVDPYASDDVIQAAFRPTTRAVFGETIANPALA
                     VFDIERFARVAHANGVPLIVDNTFPTAVLCRPFEFGADIVTYSTSKYLDGHAVSLGGM
                     IVDGGRFDWRASGKFPGLTEPDPSYHGTVYTDAFGSSAFIVKARVQLMRDLGATPSPM
                     NAFLLNLGMETLHLRMERHCRNAEAVAAFLEGHPSVSWVNYPGLPTSKDAALVKKYLP
                     CGTCGVISFGVKGGRDAAVHFMDSLKLAAIVVHVADARTGVLHPASTTHRQLTDEQLA
                     AAGIGPDLVRLSVGIENAADIIADLDQALIACD"
     gene            complement(1584..>2018)
                     /locus_tag="PLZ74_11415"
     CDS             complement(1584..>2018)
                     /locus_tag="PLZ74_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOR98968.1"
                     /translation="PGSAAGGIKTTTLVVLIMTVIAMCRNRRDTVLFNRTLPYTVVRE
                     AVVIFLLMLSLIAVAYGVLLLTEYPQQGDDASKLFFETVSAVGTVGLSIDHTGRLSNA
                     GRWVIIVCMYVGRLGPLAVALLIGSRDECQRIRYPEEEVIVG"
BASE COUNT          399 a          535 c          692 g          392 t
ORIGIN      
        1 ttgttgccga tggctacgac cacgacatcg cactcttgga aaccggcatc ttccagcgtg
       61 cgccggttgg tcacgtcgcc ctcgacggcg cgggtgacga actcggaaag ctcttggatg
      121 atttcacggt tttggtcgat cagcagaacc tccgcaccgt gttccgcgag gctttccgcg
      181 agtgatgatc cgaagcgtcc tgcgccgatg atgccgatac gtttcatggt gtttccgaag
      241 ttcgttatgg tgggttaatc gcacgcaatc agggcttgat cgaggtcagc gatgatgtcc
      301 gccgcgttct cgatgccgac ggagaggcgc acgaggtccg gaccgatgcc ggctgcggcg
      361 agttgctcat ccgtgagctg gcggtgggtt gtgctggcgg ggtgcagcac gccagtacgg
      421 gcgtcagcaa catgcacgac gatggccgcg agtttgaggc tgtccatgaa gtgtactgca
      481 gcatcccgtc cgcctttgac gccgaaagag atcacgccgc aggtaccgca cggcagatat
      541 tttttgacga gcgcggcgtc ctttgaagtg ggcaggccgg ggtagttgac ccaggaaact
      601 gaggggtgcc cttcgagaaa ggcggcgacc gcctcggcgt tgcggcagtg gcgttccatt
      661 cgcaggtgca gcgtctccat accgaggttg agcaggaagg cattcatcgg cgagggggtc
      721 gcgccgaggt cgcgcatgag ctggacgcgg gccttgacga taaaggcgga agatccgaag
      781 gcgtcggtgt agacggtgcc gtggtaggag gggtcgggtt cggtgagccc cgggaacttg
      841 ccgcttgccc gccagtcgaa gcgtccgccg tccacgatca tgccgccgag cgaaaccgcg
      901 tgcccgtcga ggtatttgga ggttgagtag gtgacgatgt ccgcgccgaa ttcgaagggg
      961 cggcagagca cggcggtcgg gaaggtgtta tcgacgatca gcggaacccc gttggcgtga
     1021 gccacgcgcg cgaagcgctc gatgtcgaag accgccagcg cggggttggc gatggtctcg
     1081 ccgaagaccg cgcgggtggt gggacggaag gcggcttgga tcacgtcgtc ggaagcgtaa
     1141 gggtcaacat aagtgacctc gatgcccagc tttttgaggg tgacgctgaa gaggttggtg
     1201 ttaccgccgt agatggcact tgaggcgata aagtggtcgc cgctattaca gaggttgagg
     1261 atcgcgatca gcgatgcagc ctggcccgag gaggtcatca gcgcaccgac gccgccttcc
     1321 agcgcggcga tcttgttctc gaccgcttca gccgtgggat tactgagccg ggtgtagaag
     1381 tggccgggca ctttcaggtc aaagaggttg ccgacatgct cgctgctatc gtacttgaag
     1441 gtcgtactct gaaagacggg caacacgcgc ggctcgccgt ttttcggctg ccagccggcc
     1501 tgaacgcaaa gggtgtctgg atggaaggac ataacagtta ggatttagga gttagcattt
     1561 aggagttagg atttaggagt tggttagccg acgatgactt cctcttcggg ataacggatg
     1621 cgttggcatt catcgcggct accgatcagt agcgcgaccg caagagggcc taagcgtccg
     1681 acgtacatgc acacgataat aacccatcgg ccggcatttg acaaccttcc cgtgtggtca
     1741 atggagaggc ccacggtgcc cacggcggag acggtttcga agaaaagttt cgaggcgtcg
     1801 tcgccttgct gcggatattc ggtgagcagc aacacgccat aggccacggc gatcagagac
     1861 aacataagca gaaaaatcac gaccgcctcg cgcacaaccg tgtagggcaa cgtgcggttg
     1921 aagaggacgg tgtcgcggcg gttgcggcac atggcgatca ctgtcatgat cagtaccacc
     1981 agcgtggtgg tcttgatgcc acccgcagcg gaaccggg
//
LOCUS       DAOSRB010000333         2016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_51292_length_2016_cov_6.610913, whole genome shotgun sequence.
ACCESSION   DAOSRB010000333 DAOSRB010000000
VERSION     DAOSRB010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51292_length_2016_cov_6.610913"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1950
                     /locus_tag="PLZ74_11420"
     CDS             127..1950
                     /locus_tag="PLZ74_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOR98969.1"
                     /translation="MRTPLESLVPRPRRIESGPGVLALGEAGVIVGAGAPDDLRPALR
                     VLEAALGSGWPLSEARASSPAGGVRLVFRIVPQVAGPQSYCLRVTSAGVEAGAQDRAG
                     LFYAAQTFAQLARLSGGVLPVCEIRDAPDFPVRGVMLDISRDKVPTMDTLFGLVDMLA
                     GLKVNQFQLYTEHTFAYRAHEEVWREASPMTPDEIRALDAYCRGRCVELVPNQNSFGH
                     LERWLTLPRYNGLAELPQGGAPLPWGGVHEKPASLCPTDPRSVDFLAGLYDDLLPNFT
                     SRLFNVGCDETFDLRGNGRSQALVRQVGEGRVYLDFLKTIHGLVTARGKRMAFWGDIV
                     IRYPELIAELPRDVLALEWGYEADHPFDAHCERFAAAGVPFYVCPGTSSWNSLAGRTE
                     NMLGNLRSAAENGLKHGACGFLIADWGDGGHWQPLCVSFAGFLYGAALSWHVEGNREL
                     PLAAALDTRLTEAGRGQLLLGLGDVYRLCGALRGNGTELFQILSKPQSRPVAQGVTGK
                     TLCAVLSRVEELERSMLTAQRSVVEQETEQVIRLLRAACHRGLALLDGSVRRAATRRA
                     LRTETDDLACAHARVWRLRNREGGLADSLAKLTRHRQEYAE"
BASE COUNT          324 a          558 c          734 g          400 t
ORIGIN      
        1 gcgtcgaatc gctcaacgcc ggcgtagccg gcgcgatcgc actctacgag gtggtaaggc
       61 agcgggggca ggcgagtcga tagaatcgac ggaatcgatt ctgttgattc ggtcgaaatc
      121 aacaccatgc gaactccatt agagagtctg gtgcccaggc cacgccggat tgagtcgggg
      181 ccgggagttt tggcactggg cgaggcgggt gtcattgtgg gcgcgggtgc gccggacgat
      241 ctgcggcccg cgctgcgggt gcttgaggcg gccttggggt cagggtggcc actgtcggaa
      301 gcgcgggcat cctcgccggc cgggggcgtc cgtctcgtgt tccggattgt gccgcaggtg
      361 gccgggccac agtcctattg tctgcgcgtc acgtccgcag gtgtcgaagc cggggcgcag
      421 gatcgggcgg gattgtttta tgctgcccag acgttcgctc aactcgcgcg cttgtcgggt
      481 ggggtactgc ccgtctgcga gatccgggat gcgccggatt ttccggtgcg gggcgtgatg
      541 ctggacatca gccgtgacaa ggtgccgacg atggacaccc ttttcgggct ggtggacatg
      601 ctggccggac tgaaggtgaa ccagttccaa ctctacaccg aacacacgtt cgcgtaccgg
      661 gctcatgagg aagtgtggcg cgaggcgagc ccgatgacgc cggatgagat ccgcgcgctc
      721 gacgcctatt gccgcggacg atgtgtggaa ctggtgccga accagaactc gttcggccat
      781 ctcgaacgct ggcttaccct gccgcgttac aacgggttgg cggagttgcc gcagggcggc
      841 gcgccgttgc cgtggggcgg cgttcacgag aagccggcct cgctctgccc gaccgatccg
      901 cgcagcgtcg attttctggc cgggctttac gacgatctgt tgccgaattt tacctcgcgc
      961 cttttcaatg tcggatgcga tgagacgttt gacctgcggg gcaacgggcg ctcgcaggcg
     1021 cttgtgaggc aggtgggcga ggggcgcgtg tacctcgatt ttctcaaaac gatccatggg
     1081 ctcgtgaccg cgcgcggcaa gcggatggcg ttttggggcg acatcgtgat ccgctatccg
     1141 gagctgattg cggagctgcc ccgggacgtg ctggcattgg agtgggggta tgaggcggac
     1201 catccctttg acgcgcactg cgagcggttc gcggcagcgg gcgtgccgtt ctatgtgtgc
     1261 cccggaacct cgtcgtggaa cagtctggca ggccggacgg agaacatgct cggcaacctg
     1321 cgttcagcgg ccgagaacgg gctcaagcat ggcgcgtgcg gattcctgat cgccgactgg
     1381 ggtgacggcg ggcactggca gccgttgtgc gtcagctttg cgggtttttt atatggggcg
     1441 gcgctatcgt ggcatgtgga gggtaaccgg gaactgccgc tggccgcagc gctggacacg
     1501 cgcttgacgg aggcgggacg gggccagctg ctgctcggat tgggtgacgt ttaccggttg
     1561 tgtggcgcgt tacgcgggaa cggtacagaa ctgtttcaga tcctttcgaa accgcagtca
     1621 cgcccggtgg cgcagggggt gaccggtaag acgctttgcg ccgtgctgag ccgagtggag
     1681 gagttggaac gctcaatgct cactgctcaa cgttcggtgg ttgagcagga gacggagcag
     1741 gtgatccgtc tgttgcgtgc ggcctgccac cgtggcttgg ccttgcttga cggaagcgtg
     1801 cgccgcgctg ccacccggcg cgcgttgcgg acagagacgg atgatttggc gtgcgcccat
     1861 gcccgtgtct ggcggttgcg aaaccgtgag ggggggctgg ccgacagctt ggcgaagctc
     1921 acacgccacc gtcaggaata cgcggagtga cttcgtcgtt caagcgtttg aacagattca
     1981 tgatttcaac actggaacac aaaaccttgc ctataa
//
LOCUS       DAOSRB010000334         2004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_51722_length_2004_cov_14.253463, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000334 DAOSRB010000000
VERSION     DAOSRB010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51722_length_2004_cov_14.253463"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="PLZ74_11425"
     CDS             complement(<1..1512)
                     /locus_tag="PLZ74_11425"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOR98970.1"
                     /translation="MKRFQKKMVYGMAGVLLFAGCRSDKGGDFQADAPKDGVTFYVST
                     KGNDAWSGSLPKPNWMGTDGPFATVTRARDAVRALKASGPLPAPVTVRLRGGRYYVTE
                     PLRFGAEDSGTESCPVTYAAYRGETPELIGGRQLTGFQSAKNGQVSLALPEVKAGTWS
                     FRSLFVNDERQVRARFPNIDPADPYRRGFLYNGVRSGLGNVVGCIHNVGDWMEYKMTV
                     PAADDYTFWMFYAAMNDSPSMKAGMSERTELIVDNGTPIPLRNLPDTGGWSTFKWSAA
                     ATVNLTAGKHVMRWRNVKGGGLDLVAYAFCDDPAWKPVDETPSAVAAGRHLVLVRASD
                     FVASQGKQLTVSGFSGKKSASLISCPPDVFRPAWLAPDVELHIYQSGSCRAFMEIVSI
                     QSYDPETHDVKLQGPECLAPLAAGDRYYVENVFEELDSPGEWYLNRQTGVLSYMPKLG
                     FTDKSEVIAPTVKRIFDVQGVDGKEPVTYLRLSGLTLRCTDWELGDGSAGYGMG"
BASE COUNT          413 a          612 c          546 g          433 t
ORIGIN      
        1 gcccatgccg tacccggcgc tgccatcacc caactcccaa tccgtgcagc ggagggtcag
       61 cccggacaga cgcaggtacg tcaccggctc ttttccatcc acaccctgaa cgtcaaaaat
      121 gcgcttcaca gtcggcgcga tcacttcgga tttgtccgtg aagcccagtt tgggcatgta
      181 gcttagaacg ccggtctgcc gattcaaata ccactcgccg gggctgtcga gttcctcaaa
      241 gacattttcg acgtagtaac gatcccccgc cgccagcggt gccagacact ccgggccttg
      301 caacttgacg tcatgggttt ccggatcata gctctggatc gaaacaatct ccatgaacgc
      361 ccggcacgag ccggactgat agatgtggag ttcaacgtcg ggtgccagcc aagcgggtct
      421 gaagacatcg ggcggacacg agatcaggct ggccgacttc ttgccactga aaccgctcac
      481 cgtcaactgc ttcccttgcg aggcgacgaa gtcagacgct ctgaccaaaa cgagatgacg
      541 tccggcggcg acggctgacg gagtctcgtc tacgggtttc caggccgggt cgtcgcagaa
      601 cgcataggcc accaagtcca gccctccgcc tttcacattc cgccagcgca tgacatgctt
      661 accggccgtc aggttgacgg tcgcggcggc gctccactta aaggtgctcc acccgcccgt
      721 gtcgggcagg ttccgcagcg ggatcggcgt gccgttgtcc acgatcaatt cggtgcgctc
      781 cgacataccc gccttcatcg aggggctgtc gttcatcgcc gcgtaaaaca tccagaaagt
      841 gtaatcgtca gcggcaggaa ccgtcatctt gtactccatc caatcgccga cgttatggat
      901 gcagccgacc acattcccca acccactgcg gacgccgttg tacaagaagc cccgccggta
      961 cggatcggca ggatcgatgt ttggaaagcg tgcccggacc tgccgctcgt cgttgacgaa
     1021 cagcgaccgg aacgaccatg tgcctgcctt gacctcaggc agcgcaagcg acacttgccc
     1081 gtttttcgcc gactggaacc cagtcaattg ccgaccaccg atcagctcgg gcgtttcccc
     1141 tcggtatgcg gcgtacgtga ccggacacga ttcggttccc gagtcttcag ccccgaaccg
     1201 gagcggttcg gtgacgtagt agcggcctcc gcgcagccgg accgtcaccg gagccggaag
     1261 cggtccggac gctttcagcg cacggacggc atcgcgcgcg cgggtcactg tggcgaacgg
     1321 cccatccgtt cccatccagt tcggcttggg caaactgccg gaccaggcat cgttgccctt
     1381 ggttgagaca taaaacgtga cgccgtcctt aggcgcatcc gcctgaaagt ccccaccctt
     1441 gtcagaccgg caccccgcga ataacagaac ccctgccatt ccgtaaacca tttttttctg
     1501 aaaccgtttc atccaacatc cctcgctttt tctgataact taatgatttc agtatacggg
     1561 aagaggacac aaagaatcaa gggcactcgt gtgcatgaat gtaatcggtt cgtgacaggg
     1621 gagtgcgaag atcatgcgtt tctgatgaaa aaggaagcag agatttggag tcgggcgtcc
     1681 tcgcccgtaa cagcgtgtct tgcggtttct gttttatccg cagattgcgc aggttttccc
     1741 ttttttgttt ttgaccgcag gggggaggga cgagcgtctg ttcgtccgcg ggcaagcaga
     1801 cgcttgcccc tcccggtgct ccgcgaggaa aacgtatcac ctattaccca ttacttctta
     1861 cttctgcccc cgcctgttag ttgactaaaa attaaaaaag gttgattcct gtattgactt
     1921 tattggtttt tcggttcaca ttaaacatgt tcaatgtggc gtatggagcg tgacggaagt
     1981 gtttgtggtc tttcactcaa ggag
//
LOCUS       DAOSRB010000335         1977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_52773_length_1977_cov_4.574402, whole genome shotgun sequence.
ACCESSION   DAOSRB010000335 DAOSRB010000000
VERSION     DAOSRB010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52773_length_1977_cov_4.574402"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1203
                     /locus_tag="PLZ74_11430"
     CDS             247..1203
                     /locus_tag="PLZ74_11430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOR98971.1"
                     /translation="MKRVLLMLLAMGCCIGDVWAQGAKTTRRAPAKKTAAPAQPQKVK
                     GCISRSPYIGAVILDADSGAVLFEERANVPGYPASTLKLMTLLVIQEKIEAGQIRLDD
                     QVKVSVEAYKTGGSQVYLDPKETFTVEEMLYAMIIQSANDAAVALAEHVAGSKEAFLV
                     LMNARAEALGMRDTRFASVHGLPPSAGQRHDVSTARDMAILARELCRYPAIFAYTSAT
                     YRQLRANTPNPFDMRTHNPFLKEGVEGCDGFKTGYTATAGWSIVVTAKRNGRRVIVVV
                     LGSEGRKQRDAEARALLNRAFTLLQPEGAPAQAVPHPASVAR"
     gene            complement(1209..1769)
                     /gene="thpR"
                     /locus_tag="PLZ74_11435"
     CDS             complement(1209..1769)
                     /gene="thpR"
                     /locus_tag="PLZ74_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOR98972.1"
                     /translation="MRLFLALSLPDSVASRLTDAVRRLEPEATDVAWCRRDQFHVTLA
                     FLGDTAPAILPHLTAACERVCSACPSFSCRAYGFGFFGSKRSPRTLWAGVDPIPELMA
                     LQEAIWQTLPKFGFADNETHFCPHITLGRCRKGARNLRLIEAMDAEDQTDFGSWRVTR
                     VTLYESRPSPRGPIYRAASLFPLATN"
BASE COUNT          438 a          544 c          635 g          360 t
ORIGIN      
        1 ccctcgacct tttggtcccg aaccaaacgc gctaccaggc tgcgctacgc tccgagaaaa
       61 accgtaaacc ggcgcataca ttagccgaac cgcaggtaaa gtcaaatcga aaaaggagcg
      121 tggaaacgat tttttgtacc gaggtctttc caatcggcgg caaacatgat taactacgcg
      181 tcaattgttt gacgccgcag ggtatgcggg gtcttggccg caggcgtaaa gaaaaaaggg
      241 ggaagggtga agcgggttct gctcatgttg ttggctatgg ggtgttgcat cggggacgtg
      301 tgggcgcagg gcgcaaaaac gacaagacgc gcacccgcca aaaaaacggc cgcgcccgcc
      361 caacctcaga aagtcaaggg atgcatttcc cggtcgccct atatcggtgc cgtgattttg
      421 gacgcggatt ccggagcggt gttatttgag gagcgcgcca atgttccggg atacccggcg
      481 agcacactga aactgatgac cttgctggtg attcaggaga agatcgaggc ggggcagatc
      541 cggttggatg atcaggtgaa ggtttcggtc gaggcgtaca aaaccggcgg ttcgcaggtt
      601 tatctcgatc ccaaggagac gttcacggtg gaggagatgc tgtacgccat gatcatccaa
      661 tccgccaacg atgcggcagt tgcgctggcc gaacacgttg cgggtagcaa ggaggcgttt
      721 ctcgtgctga tgaatgcccg tgcggaagcg ctcgggatgc gggatacgcg attcgcctcc
      781 gtgcacggac tgccgccgtc cgcagggcag cggcacgatg tctcgaccgc ccgtgatatg
      841 gccatcctcg cgcgggaact gtgccggtat ccggcgatct tcgcctacac gtcggcgacc
      901 taccggcaac ttcgcgccaa tacgcctaac ccgtttgaca tgcgcacgca caacccgttt
      961 ctcaaagagg gtgtggaggg atgcgacggg ttcaagaccg gatacaccgc gaccgcggga
     1021 tggtcgattg tggtcacggc gaagcggaac ggaagacgcg tgattgtggt ggtgctgggt
     1081 agcgaggggc gtaaacagcg ggatgccgag gcccgcgcgt tgctgaaccg ggctttcacg
     1141 ctgttgcagc cggagggagc cccggcgcaa gccgtgccgc atcccgcctc agtcgcgcgc
     1201 tgacctcttc agtttgtcgc gagcgggaac agacttgcgg cacggtagat cgggccgcgg
     1261 ggcgagggac ggctctcgta cagcgtgacg cgtgtgaccc gccacgagcc aaaatccgtt
     1321 tgatcttcgg catccatggc ttcgatcagg cgcaggttgc gcgcgccttt gcggcaacgt
     1381 cccagtgtga tgtgagggca gaagtgggtt tcgttatccg cgaaaccgaa tttcggcagc
     1441 gtctgccaga ttgcctcctg cagcgccatt agctccggta tcggatcgac cccggcccaa
     1501 agggttcggg gggaacgctt agaaccgaag aacccgaaac cgtacgcacg gcaggagaag
     1561 gacggacatg cggagcagac gcgttcgcag gcggcagtca ggtgcggaag aatagccggt
     1621 gcggtgtcgc ccagaaaggc aagggtcaca tggaactggt ctctgcggca ccaggccaca
     1681 tccgtagcct caggttccaa ccgacgcacg gcatcggtca ggcgggaggc aacggagtcg
     1741 ggcagggaca aggcaaggaa aaggcgcata tgtcgtagaa tacatcaagt ccgccggggt
     1801 ttcaatgacg accttgtcgt accgatgatc cagaatgaag ttccgtattg tgttaaaaaa
     1861 agagaacagt acttttatac ccgccgccgt tgcaaactac tgcgtggtct caactggttc
     1921 cagtcgacac cgatgacacc caacccctaa gaaatgcccc gacctgtctc caagaag
//
LOCUS       DAOSRB010000336         1955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_53621_length_1955_cov_6.729474, whole genome shotgun sequence.
ACCESSION   DAOSRB010000336 DAOSRB010000000
VERSION     DAOSRB010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53621_length_1955_cov_6.729474"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLZ74_11440"
     CDS             complement(<1..244)
                     /locus_tag="PLZ74_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOR98973.1"
                     /translation="MNTPLVPSATRTGLSAFRAVSSQTSCQDRSGFTLIELLVVIAIL
                     GILGSALVVSVQSGYKQARQANCKSNLRQFGVALTIY"
     gene            708..>1955
                     /locus_tag="PLZ74_11445"
     CDS             708..>1955
                     /locus_tag="PLZ74_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98974.1"
                     /translation="MASAVFGQPPARVLKAVPAWRVAEAPCRLVVEKGPGDFALFRVP
                     AQLDGKPVAAVRAFAATNEVAVRVVWSDASEVTLLASVGALRQKQLIRIYPVPGGQAV
                     APVAAGLTDPAPLTGVARRTAGMDFPASLADLRMLETRCDGKSVFFAVSDFSKLGTTF
                     KEWFRGDWTRKSHLVELQTWLRVPEDGRYQFGLAGVAPAWLSVDGEEIMAHPAGQPFD
                     AWTVGEDVNLRAGLRRVRIRTVCRQEIDTGLAWKRAGETGIATNVVMVTGGDLCDGRW
                     EWRNRALHPYATAQIGRSYRFRGTDEVFVPYTLRDATACWGTNYVVRWQAGTMPEQEG
                     AAAGTLPVILRKSALPTPLTLRAVSACGEKAEYTTVLQDDGRVWAEYGVTTRITDVPA
                     VCYDDDRVRPIIRIRSSALDGMAY"
BASE COUNT          327 a          540 c          723 g          365 t
ORIGIN      
        1 ggtagatggt cagggccacg ccgaactgcc gcaggttgga cttgcagttg gcctggcgcg
       61 cctgcttgta accggactgc acggagacga ccagcgcgga gcccagaatg ccgaggatgg
      121 cgatcaccac cagaagctcg atcagggtga aaccggagcg gtcttggcat gatgtctgcg
      181 aagaaacagc acggaaagca gacaggcccg ttcgggttgc gctaggaacc aatggtgtgt
      241 tcactccggt cattcctcta gttaatcgca aatatcatgc caaagacggg gtgagcagag
      301 caaggaggaa gcgcgggatt ttggaccgaa agcgcgtgtg gcatcacgga tttggacgat
      361 caaggttgaa acgtatggat actgaagtat gcaggagtgt cgttgggata ggggcgtcgc
      421 tgcgttgtgg gcaacgcagg gaacgctcaa cgtttgagga acggacgcct cggggatgcg
      481 tccctgccag ggtgcgaagc atggtagggc ggtttccccg caaaccgacg caggtccatg
      541 ccgtgagctg cggacgcctc ggggatgcgt cccgaccact tgcttcggtg ttcagcgttc
      601 gacgttcgac attcggcgtt cccgtctccc gttccccgtc accttgggtt acgggctccg
      661 cccgcgttag gtgtttggca tgggtctccc gcggtgtcgg cctctgcttg gcgtcggcgg
      721 tattcgggca gccgccggcc cgcgtgctga aggcggtgcc ggcgtggcgt gtggcggagg
      781 cgccgtgccg tctggtggtg gagaaggggc cgggcgattt cgctctgttt cgggttcccg
      841 cgcaactgga cgggaagccg gtggcggcag tgcgcgcgtt tgcggcgacc aacgaggtgg
      901 cggtgcgggt ggtctggagt gatgccagcg aggtgacatt gctggcgagc gtgggcgcgt
      961 tgcgccagaa acagctgatc cgcatctatc cggtgcccgg cgggcaggca gtcgcgccgg
     1021 ttgcggccgg tttgaccgat cccgcgccgt tgaccggggt cgcgcgcagg acggcaggga
     1081 tggatttccc cgcgtcgttg gcggatctgc ggatgctgga gacgcgctgc gacggcaagt
     1141 ccgtgttttt tgcggtcagc gatttcagca agctcggaac gacgtttaaa gagtggttcc
     1201 ggggcgactg gacgcgtaaa agtcatctgg tcgaattaca gacttggttg cgggtgccgg
     1261 aggacgggcg ttatcagttc gggctggctg gcgtggcgcc cgcctggctg tcggtggacg
     1321 gggaggagat catggcccat ccggcgggcc agccgttcga tgcctggaca gtcggggagg
     1381 atgtgaacct ccgggccggg ttgcggcgcg tgcgcatccg cacggtctgc cgtcaggaga
     1441 tcgacaccgg tctggcttgg aagcgcgcgg gtgaaacggg cattgccacc aatgtggtga
     1501 tggtcacagg cggggatttg tgcgacggcc gctgggagtg gcgcaaccgc gcgctccatc
     1561 cgtacgccac ggcgcaaatc ggccgttcct accggttcag gggaacagac gaggtgtttg
     1621 tgccctacac attgcgcgac gccacggcct gctggggaac caattatgtc gtccgctggc
     1681 aggcgggcac gatgccggaa caggaggggg ccgccgcagg aaccttgccg gtgattttac
     1741 gcaaaagcgc gttgccgacc ccgctgacgt tgcgggccgt ctcggcatgc ggcgagaagg
     1801 cggaatacac gaccgtgctt caagacgacg ggcgggtctg ggctgaatac ggtgtgacca
     1861 cccggatcac cgacgtgccg gcggtctgtt atgacgacga ccgcgtccgc ccgatcatcc
     1921 ggatccgttc ctcggcgttg gacggcatgg cctac
//
LOCUS       DAOSRB010000337         1946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_53969_length_1946_cov_9.153358, whole genome shotgun sequence.
ACCESSION   DAOSRB010000337 DAOSRB010000000
VERSION     DAOSRB010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53969_length_1946_cov_9.153358"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLZ74_11450"
     CDS             <1..248
                     /locus_tag="PLZ74_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98975.1"
                     /translation="VGSGVAKEHGDDTAASRELHRLVVYEQEQPMLMQEGLFRLTLPP
                     ELMGRGADPHNPITWHLEVKGEIPRGLDIVETYRLIS"
     gene            616..>1946
                     /locus_tag="PLZ74_11455"
     CDS             616..>1946
                     /locus_tag="PLZ74_11455"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOR98976.1"
                     /translation="MKLALSLVGSATKAGLKKRQIAERLRNLVAAPDAFLEDPRFGVL
                     AKAMLEQRASESSPASAPYGKRESDAPWCMWGSDIDPAAIEQMQNACRLPVSVQGALM
                     PDAHVGYGLPIGGVLATDNAVIPYAVGVDIACRMKLTVIDMPVNSLERHRDELIRVLE
                     SQTLFGAGREFSKPHDHPVMHEDWDFCKAVAQTKSKARGQLGTSGSGNHFVEYGTLTL
                     PEAIRDLPAGTYLAILSHSGSRGAGSIIANHFSKVARDLHKKLPRQMQHLGWLSLTSD
                     AGRDYWRAMHLMGMYAAANHEIIHHKLTRALKSRVLFQMENHHNFAWKETHGGRNVIV
                     HRKGATPAGVGVLGMIPGSMASPGFLVRGKGEAASLNSAAHGAGRLMSRSEAKNRFAW
                     SNVRRELEAAGITVLSAGVDEVPGAYKDIHKVMREQADLVEIIARFDPKIV"
BASE COUNT          407 a          544 c          639 g          356 t
ORIGIN      
        1 tggtgggaag cggcgtcgcc aaggaacatg gcgacgatac agcggccagc cgcgaacttc
       61 accggctcgt cgtctacgag caggagcagc ccatgctgat gcaggagggc ttgttccggc
      121 tcacgttgcc gccggagctc atggggcggg gtgcggatcc gcacaacccg attacctggc
      181 acctcgaggt caagggggag ataccacgcg ggctcgatat cgtggaaacc taccgcctga
      241 tcagctagcg acagaggatg tagaaaatct ggagtgcggt gacaagagcc gcgctgtgca
      301 cgggtcgcgg caccgctttt gcgcaccgct ttgccgccac actcgtgctc aaaaagcggc
      361 atcgcgcgga ataccgcttg ccgccgcact ccaatttttt cgcctgactt gcggagcatc
      421 ggtatcggaa ttgggatcgc tatcggaatc ggcgtcacat tcgtatttcg tgaatgagaa
      481 aacccaaaag gaaaaatctg cggatgaaac tccgaaacaa atccctgcgt tgcaaccact
      541 cgaaatgctc ggtataatct gggtaaaatg aaggcgaagg atatacagaa ggccggtgtt
      601 tcgtcggggc cgttgatgaa actggctttg agtttggtgg ggtcggcaac gaaggcagga
      661 ctgaagaaga ggcagattgc ggaacgtttg cgaaacctgg tcgcggctcc ggatgcgttt
      721 cttgaggatc cgcgtttcgg tgttctggcg aaagcgatgc tggaacagcg tgcctcggag
      781 tcatccccgg catccgctcc gtatggcaaa cgagagtcgg atgcaccatg gtgcatgtgg
      841 ggcagcgaca tcgatccggc cgcgatcgag cagatgcaga acgcatgtcg gttgcctgtg
      901 tccgttcagg gtgcgctgat gccggatgcg catgtcggtt acggcctccc gattggcggg
      961 gtgctggcca cggacaatgc ggtcataccc tatgccgtag gtgtggatat cgcctgccgg
     1021 atgaagctga cggtgatcga catgccggtc aacagtcttg agcggcaccg ggacgagctg
     1081 atccgggttc tggagtccca gacgctcttc ggtgcaggcc gtgagttctc caagccgcac
     1141 gaccatccgg tgatgcacga ggattgggat ttctgtaagg cggtcgccca aaccaagagc
     1201 aaggcccggg ggcagcttgg aaccagcggt tcgggcaacc acttcgtgga gtatgggacg
     1261 ctgacgctgc cggaggctat ccgcgatctt ccggcgggca cctatctggc catcctgtcg
     1321 cacagcggca gccgcggtgc cggttcgatc atcgccaacc acttctcgaa ggtggcgcgg
     1381 gatctgcaca agaaactccc gaggcaaatg cagcacttag gctggctctc gttgacgagc
     1441 gatgccgggc gggattattg gcgcgccatg cacctcatgg gcatgtacgc cgcagccaac
     1501 cacgagatca tccaccacaa actcacacgg gcgttgaaga gccgggtgct gtttcagatg
     1561 gagaaccacc acaacttcgc atggaaggag acccacggcg gacgcaatgt gatcgtgcac
     1621 cgcaaggggg ccacgccggc gggcgttggc gttctgggca tgatccccgg ctcgatggcc
     1681 agccccggct tcctggtgcg cggcaagggc gaggcggcat cgctgaacag cgcggcccac
     1741 ggagcgggac ggttgatgtc gcgcagcgag gcgaaaaacc ggtttgcatg gtccaatgtg
     1801 aggcgcgaac ttgaggcggc cggcatcacg gtcctcagcg caggtgttga cgaggtccca
     1861 ggcgcataca aggacattca caaagtcatg cgcgagcagg ccgacctcgt ggagatcatc
     1921 gcccggtttg atcccaagat cgtgaa
//
LOCUS       DAOSRB010000338         1903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_55688_length_1903_cov_9.931277, whole genome shotgun sequence.
ACCESSION   DAOSRB010000338 DAOSRB010000000
VERSION     DAOSRB010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55688_length_1903_cov_9.931277"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1236
                     /locus_tag="PLZ74_11460"
     CDS             292..1236
                     /locus_tag="PLZ74_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOR98977.1"
                     /translation="MKTCFVTRRRFLQHAGTAGLVLAAGVRADTRSRTLRLGAPAFIK
                     TDDPVELARWHRDQGYGAAYCPNVPLADTARVRAIETAFREANVVIAEVGRWCNLTDP
                     DPVKRKKNLATVTEGLALADAVGARCCVDIAGSFNPDVWYGPHPDNFTPAFFEAAVAN
                     ARTIIDAVKPKRAKFAYEMMGWCMPETPERYLALLKAVDRPSFAVHLDPCNLINSPEK
                     IYRNTDLLNACFDQLGPWIVSCHAKDLAWEIELNVHFREVRPGTGVLDYATFLRRLAA
                     LPEPPPLMIEHLKGAEEYAAAAKHIIATGSSLGIAFGP"
     gene            complement(1244..1630)
                     /locus_tag="PLZ74_11465"
     CDS             complement(1244..1630)
                     /locus_tag="PLZ74_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOR98978.1"
                     /translation="MRSFGVQTSTRSQCINITDQVQEAVQALGITDGVITVFTPHTTC
                     AVTINENADPHVVGDILAQLEALAPWENATYKHAGGNTAAHMKATLVGSSVQVIVTSG
                     SLQLGMWQGIFLCEFDGPRIRHVWVQ"
BASE COUNT          374 a          633 c          506 g          390 t
ORIGIN      
        1 aggccgcatc gcagccgccg cagacatccc ctcatcacaa cggatacggt ccccgcccgc
       61 atccatccca tcacatcgcc cggttccatt catcggtaga ttcctcctat tgccaagtgc
      121 cccgactttc cctctcgggc actcttcatc cccaaacacg catctttcca actgtttctg
      181 attttagtgg ataccctgcc ggaacacaag cagaaggcac cccgattccg cgtctgattc
      241 cgcatcgtcc cgtccttgac ctcgccctac gggtgtgcca ggatatatac catgaaaacc
      301 tgcttcgtaa cgcggcggcg tttcctccaa cacgcgggaa ccgccggact ggtattggct
      361 gccggtgtgc gagcggacac ccgttcccga acactacggc tgggcgcacc ggctttcatc
      421 aagacggacg accccgttga actggcccgt tggcatcgcg atcaagggta cggtgccgcc
      481 tattgcccca atgttcccct ggcggataca gcgcgtgtcc gcgcgatcga aaccgccttt
      541 cgcgaagcga atgtggtgat cgccgaagtc ggacgctggt gcaacctgac cgaccccgat
      601 ccggtaaaac gaaaaaagaa cctcgccacc gtcacggagg gcctggcgtt ggccgatgcg
      661 gtcggcgcgc ggtgctgcgt tgacatcgcg ggctccttca acccggatgt gtggtacggc
      721 ccgcaccccg acaatttcac gcccgccttt ttcgaggccg ccgtcgcaaa cgcccggacg
      781 atcatcgacg cggtcaaacc gaagcgcgcg aagtttgcgt acgagatgat gggatggtgc
      841 atgcccgaaa cgccggaacg ctatctcgcg ctgctcaaag cggtcgaccg ccccagcttc
      901 gcggttcatc tggatccctg caatctcatc aactctccgg agaagatcta tcgcaacacc
      961 gacctgctca acgcctgctt tgaccagcta ggaccgtgga tcgtgagttg ccacgccaag
     1021 gatctggcgt gggagatcga gttgaacgtc cacttccggg aggtacgccc cggaaccggc
     1081 gtgctcgact acgccacctt cctgcggcgg ctcgcggctc tgccggaacc gccgccgttg
     1141 atgatcgaac acctgaaggg cgccgaggag tacgccgcag cggcgaagca catcatcgcc
     1201 accggctcgt cactcggaat cgccttcggg ccgtaaccgt gcgtcactgc acccacacat
     1261 gccggatgcg ggggccatca aactcgcaga ggaaaatgcc ctgccacatg ccgagttgca
     1321 agctgccact cgtcacgatg acctgcacgg aagaaccgac cagcgtggct ttcatgtgcg
     1381 cggccgtgtt cccgccagcg tgcttgtagg tcgcattctc ccaaggcgcg agggcctcga
     1441 gctgcgccag aatatcgccc accacatgcg ggtccgcgtt ctcattgatg gtcaccgcgc
     1501 aggtcgtgtg aggcgtgaag accgttatga caccatcggt gatgcctaga gcctgaactg
     1561 cctcttggac ctgatcggtg atgttgatgc actgactgcg agtgcttgtt tgaacaccaa
     1621 aacttctcat gttcgcctcc tttcacctgc ctgctgacca caagttgaac tccattggtc
     1681 ccatcatagt ctctccgccc cctttcgggc aagatcaaaa agggggggga cagcattcgt
     1741 gtaacgtccg ccaaaaaact cgccactaac cattcgccat cacttcggcg ttgggcgttc
     1801 gacgttcagc attctacgtt cttattcttt cctcccttga cctctcccaa cgcatgtcgc
     1861 atacttgtgg cacatcattt ttttgaagga cgttatgacg cta
//
LOCUS       DAOSRB010000339         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_60510_length_1798_cov_6.683305, whole genome shotgun sequence.
ACCESSION   DAOSRB010000339 DAOSRB010000000
VERSION     DAOSRB010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60510_length_1798_cov_6.683305"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..>1798
                     /locus_tag="PLZ74_11470"
     CDS             410..>1798
                     /locus_tag="PLZ74_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98979.1"
                     /translation="MNVNQRGMLCACAIAAGMVCGMPPRPEGVVPPGSALEVPVWTVA
                     EGAPQIYEHSREAGPDETLFLVGTNLTREVTLWGTDPNHLQGKSQRPAVQLASETSLA
                     LTVPERAFDGPLVLAVKNTKGFSEPIVLNAPQPWWTSPSVAQPGTLLRVFGRNLARRP
                     DHRQAFVWLAPEGARGFWLEVVEAGKYTLTVRLPDDLAPGRHALWVHAGRGGDWGWGE
                     PVTVEITAAVRLPTRSRRLRARRDALLPIQTELDALAAKGGGTLYLPAGTHEFRGTLV
                     IPEGVVLRGAGREATTLQLVPGPAGDFTRFVTEAWGAGPSSVHTPGDEITYEVTIPEA
                     GRWQIWLRYGTDMSPWKLPGVSGHAALRFGEGPPVTLENLPNTGGFGVFKWSRSAEVE
                     LKAGRQTMVWRNIHGRGITLDAFVLRRESASAPSDKPWPVNGPGCVVIQAEACSRFQC
                     REGSLPNLNRAAV"
BASE COUNT          308 a          492 c          683 g          315 t
ORIGIN      
        1 gggggggggg ggggggggtg gtgggggggt gagggggtga ggctgtgagg gggttgtgtt
       61 ctgtggttaa gcagtcgatc ccgataccga tagcgatacc gatagcgata ccgatcccga
      121 tttggatgaa accctgaaac ccgaaaccct gaaaccctga aaccctgaaa ccccgaaacc
      181 ctgaaacctt gaaaccaaag agggttgaag ggtcaactgc tctttttaag gcgtccgcgg
      241 acggttcggg gaaccgtccc taccagcggg cgtccctgtc gatcgtcgca ggtcgaatgt
      301 cgcgggtcgc aagtcagacg tgcctttttt cctgtcatcc ctcgacaaag cagagagaaa
      361 aggggaaact gagggcaaca ggacggaatg gaaaagggag gcggatgtga tgaatgtaaa
      421 ccagcggggg atgttgtgcg catgcgcgat tgcggccggg atggtgtgcg ggatgccgcc
      481 gcggcctgag ggtgtggtgc cgccggggag cgcgctggag gtgccggtct ggactgtggc
      541 cgagggagcg ccgcagattt atgagcacag ccgcgaggcg ggaccggatg agacgctttt
      601 cctggtcggc accaacctga cgcgcgaagt gacgctgtgg ggaaccgatc cgaaccatct
      661 gcagggcaag tcgcagcgcc ccgctgtaca actggcctcg gaaacctcgc tggcgttgac
      721 cgtgccggag cgggcgtttg acggtccgct ggttttggcg gtcaaaaaca cgaaaggctt
      781 ttcggagccg atcgtgctca atgcgccgca accgtggtgg acctcgcctt cggtcgcgca
      841 accgggcacc ttgctgcgcg tgttcgggcg caatctggcg cgtcggcccg atcaccgtca
      901 agcgtttgtc tggctggctc cggagggcgc gcggggcttt tggctggaag tggttgaagc
      961 ggggaagtac acgctgacgg ttcgtttgcc ggacgatctc gcgcccggtc gccatgcgct
     1021 ttgggtccat gccgggcgcg gcggggattg gggttggggc gaaccggtga ccgttgaaat
     1081 aacggcggcg gtgcggttgc ccacccggag caggcggttg cgcgcgcggc gggacgcgtt
     1141 gttgccgatc cagacggaac tggacgcgct ggccgccaag ggcggcggca cgctgtatct
     1201 gcccgccgga acacatgagt tccgggggac gctggtgatc ccggaggggg tggtcttgcg
     1261 cggtgcggga cgcgaggcca ccacgctcca gctcgtgccc gggccagccg gagatttcac
     1321 gcgtttcgta accgaggcct ggggcgcggg tcccagcagt gtgcacaccc cgggcgatga
     1381 gatcacgtac gaggtgacga tcccggaggc aggccggtgg cagatctggc tgcgctacgg
     1441 taccgatatg tcgccttgga aactgccggg cgtcagcgga catgcggccc tccggttcgg
     1501 ggaaggcccg cccgtgacgc ttgagaacct gcccaacacc ggagggttcg gtgtgttcaa
     1561 gtggtcccgc tctgcggagg tggaactcaa ggcgggacgg cagacgatgg tgtggcggaa
     1621 cattcatggc aggggaatta cgctggatgc gtttgtgctg aggcgggagt cggcctccgc
     1681 gccttcggac aagccgtggc cggtgaacgg gccggggtgc gtggtgatcc aggccgaggc
     1741 gtgctcgcgg ttccagtgcc gcgaaggttc gctgccgaac ctcaaccggg ctgcggtc
//
LOCUS       DAOSRB010000340         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_61451_length_1780_cov_6.351304, whole genome shotgun sequence.
ACCESSION   DAOSRB010000340 DAOSRB010000000
VERSION     DAOSRB010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61451_length_1780_cov_6.351304"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLZ74_11475"
     CDS             <1..446
                     /locus_tag="PLZ74_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98980.1"
                     /translation="DILENMKGLGGVEIRKNGVDWRVVVMDDTATPPAAPEIPDISVA
                     SPADGGTASGTFDVLTGLAVDGTDKHQLNPTKMSVTLPTVSGYTGTLTVIVGWETVAD
                     GTTDRMKLKTRKFTFASGLLSSIGDEVSTQWFVSTPHVAEHPGGL"
     gene            448..>1780
                     /locus_tag="PLZ74_11480"
     CDS             448..>1780
                     /locus_tag="PLZ74_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98981.1"
                     /translation="MATLGAHFGLVVNAQGSVTGGGSYEGVETAATSALVGTYSGVKL
                     YGFKFPSGRLICQPYPSADVSRAAADIRDWSSGGGQVRSVRPVLYFYNAGAWQWGVGQ
                     VMAYRTGYFNQKRLWPGPDGSTVTIFDMSGWVCWANKVTFSNDSGRAIRVRSFVPNDR
                     KGAVYVGSETSLNEVVADGDSLAITVFEGWKLQFEDSQFPDDYQVEIVPSVGAVDGDP
                     WADTPCVIDTSHDVVFDPRIPQGLEAGTEDHPESPLADIDTLTATKCVYAGRALLYGA
                     AGDPLYDSSHVGIIYRGTVLPEEPPPAPPPGPSVDYALITVSWGSGIHLDVIAYWTDV
                     PGVTVGWTVDAGNPGAAIVMHNQDNVRLGPEHITLGVTAASRLAGVTTYTFRVHFNFW
                     GGSGSGAEIEVRHGAVTLNKAVSPSTNFHVPASKSDPYVTITFNAAGTPLSI"
BASE COUNT          336 a          554 c          565 g          325 t
ORIGIN      
        1 tggacattct tgaaaacatg aaaggccttg gaggcgtcga gatccgcaag aacggcgttg
       61 actggcgcgt cgtggtaatg gacgacacgg ccacgccgcc tgctgcgccg gagatcccgg
      121 acatctccgt cgcatccccc gcggacggcg gaaccgcgtc cggcacgttc gacgtgctca
      181 ccgggttggc cgttgacggc accgacaagc atcagctcaa tccgaccaag atgagcgtca
      241 cgctgccaac cgtttccggc tacaccggaa ccctcactgt tattgtcgga tgggagactg
      301 ttgcggacgg aacgactgac cgcatgaaac tgaaaacgcg gaagttcacg ttcgcgtccg
      361 ggcttctctc gtccatcggc gacgaggtct ccacgcagtg gttcgtatcg acgccgcacg
      421 tcgcggaaca tcctggagga ctgtgatatg gcgaccctcg gagcacattt cggcctggtg
      481 gtcaacgcgc agggctcggt caccggaggc ggcagttacg aaggagtgga gaccgccgcg
      541 acatccgcgc tggtcggaac atactcgggc gtgaagctat acgggttcaa gttcccgtcc
      601 ggccgcctga tctgccagcc gtacccatcc gcggacgtat cccgcgccgc ggcggacatc
      661 cgcgactggt catcgggcgg cggtcaagtc cgttccgtgc ggccggtgct ctacttctac
      721 aacgcaggcg cgtggcagtg gggcgtcgga caggtcatgg cgtaccgcac cgggtatttc
      781 aaccagaaaa ggttgtggcc cggcccggac gggtcaaccg tgacgatctt cgatatgtcc
      841 ggctgggtat gctgggcaaa caaagttacg ttcagcaacg acagcgggcg ggcaatccgc
      901 gtgcggtcct ttgtcccgaa tgaccgcaaa ggagcggtct acgtagggtc cgaaacatcc
      961 ctgaacgagg tggtggcgga cggcgacagc ctcgcaatca cggtgttcga gggctggaaa
     1021 ctgcaatttg aggactcgca gttcccggac gattatcagg tggagattgt cccgtctgtg
     1081 ggagcggtgg acggcgaccc gtgggcggat accccctgcg tgatcgacac gtcgcacgac
     1141 gtggtttttg acccgcgaat cccgcaaggc ctcgaggccg gcacagagga tcacccggaa
     1201 tcgccccttg ccgatatcga taccctcacg gcgaccaagt gcgtctatgc cgggcgcgcc
     1261 ctgctttatg gggccgctgg agacccgctc tatgactcct cccacgtcgg catcatctac
     1321 cgcggcaccg tcctgcctga ggaaccccct cccgcgccgc cgccagggcc gagtgtggac
     1381 tacgcgctga taacggtatc atggggatca ggcatccatc tggatgttat cgcctactgg
     1441 acggacgtgc cgggggtaac tgtcgggtgg accgtcgatg ccgggaatcc cggcgcggca
     1501 atcgtcatgc acaatcagga caacgtccgc ctcggccccg agcacatcac gctgggcgtc
     1561 acggctgcgt ccaggcttgc aggggtaacg acgtacacat tccgagtgca cttcaatttt
     1621 tggggcgggt cgggatcggg ggcggagatt gaggtccgac acggcgcggt tacgctcaac
     1681 aaggccgttt cgccatccac gaactttcat gttcccgcat cgaaatccga cccctatgtg
     1741 accataacct ttaacgcggc tgggacgccg ctgtccatcg
//
LOCUS       DAOSRB010000341         1744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_63323_length_1744_cov_5.478390, whole genome shotgun sequence.
ACCESSION   DAOSRB010000341 DAOSRB010000000
VERSION     DAOSRB010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63323_length_1744_cov_5.478390"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLZ74_11485"
     CDS             <1..1028
                     /locus_tag="PLZ74_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98982.1"
                     /translation="TETLHASVDKPAPEYSREKFLIYGNEAAPNLTFSRNPSNLSNAG
                     DGFFSRWRMRRAVAKLEAFSRNLDDEYGYTIVSNRQFAALFHAIDRNDEVQFRLLFTP
                     LAQQQMLNLLRDRTVGFGDNFTFVKDRMINLVRPAHLATTDITAAPSLFHHYDLAAAR
                     EFFNTYSNAYFRALYFALAPVLTIPLYQQHRSHADIYASVLGKTASFWEHEAIANFHG
                     QHAFGHHEAITENLLKTQAVRTDDGYTDVVVTAHGFRGEHRVDYVSKFGGDGRWHKVP
                     VKWIEYIPVQRTSTLTIRETDGLTLQDYEEQSQTSPDWQTFFRKYQIEPKHVSFRRSI
                     VSFVSPS"
     gene            1303..>1744
                     /locus_tag="PLZ74_11490"
     CDS             1303..>1744
                     /locus_tag="PLZ74_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98983.1"
                     /translation="MKMLKSLVALAGVSGMALGAEFHVAKNGNDTNDGSVAKPFATVE
                     RAVTALRAVRASTPQEPVRVVIGAGTFELRAPILLTDRDTTGPVAFVGKGATQTILSG
                     GREIAPALWRIGGDGRSWAVTLPEVSNGTWRVQQAYAGGRRVLRP"
BASE COUNT          375 a          500 c          480 g          389 t
ORIGIN      
        1 gcaccgagac gcttcacgca tccgtcgaca agcctgcgcc ggaatactcc cgcgagaaat
       61 tcctcatcta cggcaacgag gccgctccga acctcacctt cagccgcaat ccctccaacc
      121 tctcaaatgc tggcgatggg ttttttagca ggtggcgcat gaggcgcgcc gtcgctaaac
      181 tcgaagcgtt ctcccgcaac ctcgacgatg agtatgggta caccattgtg tccaaccggc
      241 agtttgccgc gcttttccat gccatcgacc gcaacgacga agttcagttt cgccttctct
      301 ttacccccct ggcccagcag cagatgctta acctcttgcg cgacaggacc gtcggcttcg
      361 gcgacaattt cacctttgtc aaggaccgca tgatcaacct tgtcagaccc gcacacctcg
      421 cgaccaccga cattacggct gccccctcgc ttttccatca ctacgacctc gctgccgccc
      481 gcgagttttt caatacctat agcaatgcct atttccgcgc cctgtatttt gccctcgctc
      541 cggttctaac cattcccctt taccagcaac atcgttccca tgccgacatc tatgcgagcg
      601 tgttaggcaa gaccgcctcg ttctgggaac acgaggccat cgccaacttc cacgggcagc
      661 atgccttcgg gcatcacgaa gccatcaccg aaaacctgct caaaacgcaa gctgtccgta
      721 cggatgacgg ttacacggac gtggtcgtta ccgcgcacgg tttccgggga gagcaccgag
      781 ttgactatgt ctcaaagttc ggtggagacg gtaggtggca taaagtcccc gtcaaatgga
      841 tcgaatatat ccctgtacag cgtacctcaa cattgacgat tcgggaaact gacggactta
      901 cgttacaaga ctacgaagag cagtcacaaa cctcgccgga ctggcaaacc tttttccgca
      961 aatatcaaat cgagccgaag cacgtatcgt tccgccggtc tatcgtttcg ttcgtgtccc
     1021 cttcgtaagg cgtacctctt gccatctttc ccgtggatcg gttcccgggg gtaacgtatt
     1081 gtcttgcaca ttgaaaaccc gcgtatgggt ttggattgcg tgaatcatga ttgaacctgt
     1141 ccacatgaat gacgatgatc caaagatatg aaaaacatca aaacttaaaa ttggattcgg
     1201 tgtcgtgggg cgggttatgg tatgatgcta cattaacaga ccgtatgaac ggaatgtgga
     1261 cgtattgatt gtctagatat cttgagagag aaaggttatg cgatgaaaat gctgaagagt
     1321 ctggtggccc tggcgggggt gtcgggaatg gccttgggtg ccgaattcca tgtggccaag
     1381 aatggaaacg atacgaacga cgggtcggtg gcgaagcctt ttgccacggt cgaacgtgcc
     1441 gtgacggcgc tgcgtgctgt ccgggcctcc acgccgcagg agcctgtgcg tgtcgtgatc
     1501 ggtgcgggca cgtttgaact ccgtgcgccg atcctgttga cagaccggga tacgacggga
     1561 cctgtcgcgt ttgtcggaaa gggcgcaacg caaacgatcc tttcgggcgg acgggagatc
     1621 gcgcctgcgc tctggaggat cgggggggat gggcgcagtt gggccgtgac gttgcccgag
     1681 gtctcgaatg gcacatggcg cgtgcagcag gcgtatgcag ggggacgccg cgtgttgcgt
     1741 ccat
//
LOCUS       DAOSRB010000342         1740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_63526_length_1740_cov_4.387537, whole genome shotgun sequence.
ACCESSION   DAOSRB010000342 DAOSRB010000000
VERSION     DAOSRB010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63526_length_1740_cov_4.387537"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="PLZ74_11495"
     CDS             complement(<1..1508)
                     /locus_tag="PLZ74_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98984.1"
                     /translation="MWRLFIPLLSTAWLIATHLSVWGAVSPDAAQELCFVPAVRLARF
                     EVTADAAGTVSLAWETTLELGSGIFRVLRQETGALPDEREVARLATCDDEEGGVYRVR
                     DALARVGEACAYTIRFLPRNGTETELAVWQGTIRVSDRTTAAAVAPSPVTQSSAPAAV
                     QRWIGEGPRVRVWDDPEPADRVRVSLTEAGVYSVTLQELSEASGWPIDTLLSDLDATN
                     LCIQCQGNPVAWLADGRPRLIFHALPAASHFAPENVYWISRSPGLTMDESYQVPPVPA
                     VTNAWFTDRIERQGTSYLARVTRGTLTDAHIPFAAFPGPVLNGDSRTLPAEPLTDCAP
                     GEWSGAMTVRLVSLYEVGTDTHFARLTINGSALGDIAWTNEAYQTATFPFASASVPDG
                     TLALTLRNAAAPPPFSSTDYTRFLCLSYAIDYARRYVAHDNRLRCTGGSGSLAAVTRF
                     TTNDVLVLDVTDAARPLRVTGCKHHQDTATGLWTISFACGDATRIYRLASNS"
BASE COUNT          348 a          542 c          578 g          272 t
ORIGIN      
        1 ttcgagttgg aggcgagccg gtagatccgg gtcgcgtcac cgcacgcgaa cgaaatggtc
       61 cacagccccg tggccgtatc ctgatggtgt ttgcatccgg tcacgcgcag gggccgcgcg
      121 gcatcggtga cgtccagaac gagcacgtca ttcgtggtga agcgggtgac cgcagcgagg
      181 gaaccgctcc cgcccgtaca ccgcaggcga ttgtcgtgcg cgacatagcg ccgcgcatag
      241 tcgatggcgt aggagagaca gaggaagcgg gtgtaatccg tggaagaaaa ggggggcgga
      301 gcagccgcat tgcgaagtgt cagggcgagg gttccgtcag gcaccgatgc ggaagcgaaa
      361 gggaaggtgg ccgtctgata ggcctcgttc gtccacgcga tgtcgccgag cgcgctgccg
      421 ttgatcgtga ggcgcgcaaa atgcgtatcg gttccgacct cgtacaggga gacgaggcgt
      481 accgtcatgg cgccgctcca ctctccgggc gcgcaatccg tcaacggctc tgcgggcaag
      541 gtgcgcgaat cgccgttcaa cacaggtccc ggaaacgcgg caaacggtat gtgcgcatcc
      601 gttagcgtac cgcgcgttac gcgcgcgagg tagctggtgc cctgccgctc gatgcggtcc
      661 gtgaaccagg cgttggtgac ggccgggacg ggcggcacct gatagctctc atccatggtc
      721 aggccgggac tgcgggatat ccagtacacg ttctcgggcg cgaaatgcga tgccgcgggc
      781 agcgcgtgga aaatcaggcg cggacggcca tccgccagcc aggcgaccgg attcccttgg
      841 cattgaatgc aaaggttggt ggcgtccaga tccgacaaaa gcgtatcaat cggccacccc
      901 gaagcctccg acaactcctg cagcgtcacg ctgtacacgc ccgcctccgt caggctcacg
      961 cgcacccggt ccgccggttc cggatcatcc cagacgcgca cacgcggccc ttcgccgatc
     1021 cagcgctgta cggccgcagg cgcgctggac tgggttacgg gggaaggggc aaccgcggcg
     1081 gctgtcgtcc ggtcggaaac gcggatcgtc ccctgccaca ccgcgagttc tgtttccgtt
     1141 ccgttccgcg gcaggaaacg aatcgtgtag gcgcacgcct cgccgacacg cgccaacgca
     1201 tcccttacac ggtaaacccc gccttcctca tcatcgcaag tggccaagcg cgcgacctca
     1261 cgctcatccg gaagcgctcc ggtttcctga cgcaggacgc ggaagattcc tgagccgagt
     1321 tccagcgtcg tctcccacgc cagactgacg gtgcccgccg catctgccgt tacctcgaaa
     1381 cgcgcgagcc gcaccgcagg gacaaaacac agttcctgtg cggcgtccgg ggataccgca
     1441 ccccacacgg acaggtgggt cgcgatcagc caggcggtgg ataaaagagg tataaataaa
     1501 cgccacatag ggaaaacaaa gcctctccac acacattatc tggagttgat gcgtgtggtc
     1561 aaagtgtaca ggctccgtcc ggccgacaca accggaaact tgcgggatga cgcattcggg
     1621 gataggattg cgacgagggc gggggggtgt taagacgggg ccaagggcag gggcgacgcc
     1681 cggttatcgc gtgatgagca gcaggagccc ttgggcgatg ccgacgacga aaccaatgac
//
LOCUS       DAOSRB010000343         1716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_64806_length_1716_cov_6.092113, whole genome shotgun sequence.
ACCESSION   DAOSRB010000343 DAOSRB010000000
VERSION     DAOSRB010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64806_length_1716_cov_6.092113"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1347)
                     /locus_tag="PLZ74_11500"
     CDS             complement(109..1347)
                     /locus_tag="PLZ74_11500"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOR98985.1"
                     /translation="MNDTQLYEQILGLTPPWRVAGVTLKKELRQIEIEVICDETVWAC
                     PECRQRMHVHGSERRRWRHLDTCQFQTLVVADVPRVKCATHGTQQVRVPWAEKYSRFT
                     ALFERFAIDVLLECSIRGACELLGISWEEADGIKQRAVDRGLARKTSAAPRRVCVDEK
                     SFGRGHDYVTLVLAVEQGRAATVERITEGRTRESLEAYWRTLTPEQRVAVEAISMDIW
                     EPYAAATRACVPGAEHKIVHDPFHLLRHMNEAVNDVRKGEHRALQKLGDTRLAGSKQL
                     WLYGWENVPDRWEGRLDALRGQKLKTSRAWALKELFRDFWTCPTVEQARAFFARWYAW
                     AIRSRLGAVKRVARMFRRHLEQILTYFRHRLTNAAAEGMNNRVQSLIAKAYGYRNRER
                     FKRDILFHAGGLQLYPVCLQ"
BASE COUNT          315 a          617 c          431 g          353 t
ORIGIN      
        1 gaatatctct tcctaaacgc tcttctcttt gacattcaac agtctatcaa gtcgttttat
       61 ttctgcaccc ttttcttttt ggttcttccg taatttccgt gggaaatttc attggaggca
      121 aacagggtag agctggaggc cgcctgcatg gaagaggatg tcccgcttga accgctcccg
      181 gttgcggtag ccataggcct tggcgatcag gctctgcacc cggttgttca tcccctccgc
      241 cgcggcgttc gtcaaccggt gcctgaagta cgtcaggatt tgttccaggt gccgtcgaaa
      301 catccgcgcg acccgcttca cggctcccaa ccgactccga atggcccaag cgtaccatcg
      361 ggcaaagaac gctcgcgcct gctcaaccgt cgggcaagtc caaaagtcgc ggaacagctc
      421 cttcaacgcc cacgcccggc tggtcttcag cttctggccg cgcagtgcgt ccaagcggcc
      481 ctcccatcga tccggcacgt tttcccagcc gtacagccac agctgcttgc tccccgccag
      541 ccgggtatcc cccagcttct ggagagcccg gtgctcgccc ttgcgcacgt cattgaccgc
      601 ctcgttcata tgccgaagca gatgaaacgg atcgtggacg atcttatgct ccgccccggg
      661 cacgcaggcc cgcgtcgccg cggcgtaggg ctcccagatg tccatgctga tcgcctccac
      721 cgccacccgc tgttccggcg tcaacgtgcg ccagtacgct tccaggcttt cccgcgtccg
      781 accctccgtg atccgctcta cggtggcggc gcgcccctgt tcgaccgcca gcaccagggt
      841 cacgtaatcg tgcccgcggc cgaagctctt ctcatccaca catacccgcc ggggagcggc
      901 cgacgtcttg cgcgccaagc cccggtccac cgcgcgttgc ttgatcccgt cggcctcttc
      961 ccagctgatc cccagcagtt cgcaggcccc ccggatcgaa cactccaaca gcacgtcgat
     1021 cgcaaaccgc tcgaacagcg ccgtaaaccg gctgtacttc tccgcccatg gcacgcgcac
     1081 ttgctgcgta ccgtgcgtcg cacacttcac ccgcggcacg tccgccacca ccagggtctg
     1141 aaactggcag gtgtccagat gccgccaccg gcgacgctcc gacccgtgca cgtgcatccg
     1201 ctgccggcac tcgggacagg cccacaccgt ctcgtcgcag atcacctcaa tctcgatctg
     1261 ccgcagctcc ttcttcaagg taacgcccgc cacccgccaa ggcggggtga gcccgagaat
     1321 ctgttcgtac aactgggtgt cattcatgcc cccagtctac ccgctcaacg aaaaatcaca
     1381 cggaaatccc ggaagaacct ggatgataaa gggatgccgc cgggatccgg attggggtca
     1441 cccattagag gttccggtca aacacaaaaa gcccctctgg tccgttttta aaacggattc
     1501 actcctctcc ctgttttgaa cggatacacc gtcgccacgt ttcgaccgtc ttttccgcgg
     1561 ttttcatttc acggtttgtt cagtcaccca tctggttctc atcccgatcc cccaggcgaa
     1621 tcggaacaca tcccgccaag cgggaccaga aaaccgtttg tacctgctcg tcgcctattc
     1681 agtcacggtg gttctcccca aaagggattc cagacc
//
LOCUS       DAOSRB010000344         1700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_65695_length_1700_cov_7.483283, whole genome shotgun sequence.
ACCESSION   DAOSRB010000344 DAOSRB010000000
VERSION     DAOSRB010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65695_length_1700_cov_7.483283"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLZ74_11505"
     CDS             complement(<1..1216)
                     /locus_tag="PLZ74_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOR98986.1"
                     /translation="MKNRFLLFSLFLACLGIPAAAQTNAPAPYGPVPSERQLRWHETE
                     VFALIHYGLNTYTDREWGYGEEDPALFNPSRLDAEQVVAALKRGGMKGVILVAKHHDG
                     FCLWPTRTTEHNISRSPWRGGKGDLVREYADAAKKHGLRFGVYCSPWDRNSAAYGTPA
                     YLPIYHGQLRELLTRYGTLFEVWFDGANGGDGYYGGAREKRNIDRTTYYDWPATWGLV
                     RQLQPEACIFGDVGPDCRWVGNEKGFAAEESWATFTPKGKTDPARPANGDTMNHIEGP
                     AGHRDGTAWIPAECDVPLRKGWFWHPNEDATVRTPAQLMEIYFNSVGKGGCMNLGVAP
                     DRDGLVCAADIRSLDEFGGLLKTLFANDLARGARVAADQVRTASADAPGGGAAPFAPA
                     RVLDGDRYSYWAA"
     gene            complement(1315..>1700)
                     /locus_tag="PLZ74_11510"
     CDS             complement(1315..>1700)
                     /locus_tag="PLZ74_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR98987.1"
                     /translation="QFGMMCASSISQTAGLEAVRHGDADIAEMRDAYHLRRNFVVASL
                     NEMGLDCFMPRGAFYVFPSIQSTGLSSKEFALRLLKEHNVACVPGEAFGACGEGYLRC
                     AYATGMTQLKEAMERMGKFVSALVR"
BASE COUNT          314 a          546 c          541 g          299 t
ORIGIN      
        1 ctgcagccca gtaactgtag cggtcgccat cgagcacgcg cgccggcgca aagggcgcgg
       61 cccccccgcc cggcgcgtcc gctgacgcgg tacgcacctg atcggcggcg acccgagcgc
      121 cacgcgcgag gtcattcgcg aagagcgttt tcagcaggcc gccgaattcg tccagcgaac
      181 ggatgtccgc cgcgcacacc agtccgtcgc ggtcgggcgc cacgccgaga ttcatgcagc
      241 cgcccttgcc gacactgttg aaatagatct ccatgagttg ggccggcgtg cgcacggtgg
      301 cgtcttcgtt cgggtgccag aaccagccct tacgcagcgg aacgtcgcac tcggcgggta
      361 tccacgccgt cccgtcgcgg tggccggccg gtccttcgat gtgattcatg gtgtcgccgt
      421 tggccgggcg tgcgggatcg gtcttgccct tgggcgtgaa ggtggcccag ctctcctcgg
      481 cggcgaaccc tttttcgttg ccgacccagc ggcagtccgg ccccacgtca ccaaagatgc
      541 aggcctcggg ctggagctgg cgtaccaggc cccaagtggc cggccagtcg tagtaggtcg
      601 ttcgatcgat attgcgtttc tcgcgggcac caccgtaata gccgtcgccg ccgttggcac
      661 cgtcgaacca gacctcgaac agagtaccgt agcgggtaag cagttcgcgg agctggccgt
      721 ggtagatcgg caggtaggct ggcgtgccat aggccgcgct gttgcgatcc cagggcgaac
      781 agtagacccc gaagcgcaac ccgtgcttct tggcggcgtc ggcatattcg cgcacgagat
      841 cgcctttgcc gccgcgccaa ggcgagcgcg agatgttgtg ttccgtagtc cgggtaggcc
      901 agaggcagaa gccgtcatga tgcttggcca ccaggatcac ccctttcatg ccgccgcgct
      961 tgagcgccgc cacgacctgc tccgcatcaa gccgggacgg gttgaagagc gcaggatcct
     1021 cctcgccgta cccccactca cggtcggtgt aggtgttcag gccgtagtgg atcagcgcga
     1081 aaacctcggt ctcgtgccag cgcaactggc gctcgctcgg cacagggccg tagggcgcgg
     1141 gcgcattggt ctgcgcggcg gcggggatgc cgagacaagc caaaaacaga gagaacagca
     1201 agaaccggtt tttcacatca tgatctcccg tgcgggcgga gcccgcgatc caagttaatt
     1261 agttaattag tgcattagtt tgtctaggac gctctgaact aacgcactaa tgcactaacg
     1321 cactaatgca ctaacaaact tccccatgcg ctccatcgcc tccttgagct gcgtcatgcc
     1381 ggtggcgtag gcgcagcgca agtacccctc gccgcaggca ccgaaagcct cgcccggcac
     1441 gcacgcgaca ttgtgctctt tgagcaggcg cagggcaaac tccttggagg agaggcctgt
     1501 ggattggatc gatggaaata cgtagaacgc gccgcgcggc atgaagcagt cgagccccat
     1561 ctcattgagc gaagcgacaa caaaattacg ccgcaaatgg taagcgtcgc gcatttcagc
     1621 gatgtcggca tcaccgtggc gcaccgcttc gaggccggcg gtctggctga tcgacgaggc
     1681 gcacatcatg ccgaactggt
//
LOCUS       DAOSRB010000345         1696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_65938_length_1696_cov_3.205972, whole genome shotgun sequence.
ACCESSION   DAOSRB010000345 DAOSRB010000000
VERSION     DAOSRB010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65938_length_1696_cov_3.205972"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>1696
                     /locus_tag="PLZ74_11515"
     CDS             2..>1696
                     /locus_tag="PLZ74_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR98988.1"
                     /translation="MFSPIRVNRTTIKNRLVMAPMGNIDMAEETGRPNDKMIQYFVAR
                     AKGGVGLITTGLVPVSHGIDNSVTELGHLSYFPRIDRSRTVFAGWRDLAQGVHSFGAK
                     IFVQLTPGLGRVGNPQCLITELKFPVSASMNPNFYIPQIPCMPLSDGKLKKIIKNAGQ
                     AAADAKAAGLDGVYLHGHEGYLLEQLTNPAFNRRKLGRYADVQNFGTDLIREIRRRTG
                     PDYPVMYRIDLSLALNETYGAQMDEIPSLKKFKDGRTIPDTLAYMKNLVAAGVDMFDV
                     DLGCYDNWWLPHPPAGMPAGCFLEIAEIAKSYLKENKIFSNAGVEVPVVAVGKLGYPD
                     MAEKALREGMAAMIMLGRPLLADPEWPNKAYAGRVEEIRPCIGCQEGCINEFVDGGHP
                     QCAVNPRTAFEHVYPDIPAKADVKKKIAVVGAGPAGIVFALTAASRGHEVDLVEKGSA
                     VGGRLIPGSRPRIKFDVDNYRRYLAREAGKAAAEGKISLRLNTVLTAEELKAAGYDAV
                     VFAIGTKNAVPPIPGIEKANAVQAVDALMDDALLAGKKKAVVIGGGVVGCETAYYLK"
BASE COUNT          338 a          567 c          542 g          249 t
ORIGIN      
        1 gatgttctcg ccgattcggg tgaaccgcac caccatcaag aaccgcctcg tgatggcgcc
       61 catgggcaac atcgacatgg ccgaggaaac cggccgcccc aacgacaaga tgatccaata
      121 cttcgtcgcc cgggcaaagg gcggcgtggg gctcatcacg acggggctcg tccccgtcag
      181 ccacggcatc gacaattccg tcaccgagct cgggcacctt tcgtatttcc cgcgcatcga
      241 ccgaagccgc accgtgttcg cgggctggcg cgacctcgcg cagggcgtcc acagcttcgg
      301 cgcgaagatc ttcgtgcagc tcacgccggg cctcggccgc gtgggcaacc cgcaatgcct
      361 gatcaccgaa ctgaagttcc cggtgtcggc ctcgatgaac ccgaacttct acataccgca
      421 gatcccgtgc atgccgctct cggacggcaa gctcaaaaag atcatcaaaa acgccgggca
      481 ggccgcggcg gacgccaagg ccgcggggct cgacggcgtc tatctgcacg ggcacgaggg
      541 atacctcttg gagcagctca cgaaccccgc gttcaaccgc aggaagctcg gccgctacgc
      601 ggacgtgcag aacttcggca cggacctgat ccgggagatc cgccgccgca cgggaccgga
      661 ctacccggtg atgtaccgca tcgacctctc gctcgcgctc aacgaaacct acggcgcgca
      721 gatggacgag atcccctctc tcaaaaagtt caaggacgga cgcaccattc ccgacacgct
      781 cgcgtatatg aagaacctcg tcgcggcggg cgtagacatg ttcgacgtgg acctgggctg
      841 ctacgacaac tggtggctgc cgcatccccc ggccggcatg cccgcgggct gcttcctcga
      901 gatcgccgag atcgcgaaga gctacctcaa agagaacaaa atcttctcga acgcgggcgt
      961 cgaggtaccg gtcgtggccg tgggcaagct cggctatccg gatatggcgg agaaggcgct
     1021 gcgcgagggc atggcggcta tgatcatgct cggccgtccc ctgctcgccg accccgaatg
     1081 gcccaacaag gcctacgcgg gccgcgtgga ggagatacgc ccctgcatcg gctgccagga
     1141 aggctgcatc aacgagttcg tggacggagg ccatccgcag tgcgcggtca acccgaggac
     1201 ggcgttcgag cacgtctacc ccgacatccc cgcgaaggcc gatgtcaaaa agaagatcgc
     1261 cgtcgtcggc gcgggccccg cgggaatcgt gttcgcgctc acggcggcgt cgcgcgggca
     1321 cgaggtggac ctcgtcgaaa aaggttccgc cgtgggcggc aggctcatcc ccggcagccg
     1381 tccgcgcatc aagttcgacg tggacaacta ccgccgctac ctcgcgcgcg aagcagggaa
     1441 agcggcggcg gaggggaaga tcagccttcg cctcaacacc gttttgacgg cggaggaatt
     1501 gaaggccgcg ggctacgacg ccgtcgtgtt cgccataggc acgaagaacg ccgtgccgcc
     1561 catacccgga atcgagaaag ccaacgccgt gcaggccgtg gacgcgctca tggacgacgc
     1621 gctcctcgcg ggcaagaaga aggccgtcgt catcggcggg ggcgtggtcg gctgcgaaac
     1681 ggcgtattat ctcaag
//
LOCUS       DAOSRB010000346         1690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_66243_length_1690_cov_11.203670, whole genome shotgun
            sequence.
ACCESSION   DAOSRB010000346 DAOSRB010000000
VERSION     DAOSRB010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66243_length_1690_cov_11.203670"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /gene="glgB"
                     /locus_tag="PLZ74_11520"
     CDS             complement(<1..1310)
                     /gene="glgB"
                     /locus_tag="PLZ74_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOR98989.1"
                     /translation="MIKNDIPQDEIYLFNIGEAQQAYATFGCHRVNESGLHRFLVWAP
                     NAREVSVVGDFNGWDASRNRMERLDSGVFAAFVPGLRDGDCYKYAIHGCDGKTVLKAD
                     PFAFHAEVRPATASKVWSLGKYDWHDAEYLKRRQGQNLLNRPVSIYEMHIGSWRKPED
                     WRFADFRRVADELSDYLVEMGFTHVEIMPVTEYPLDDSWGYQVTGFYAITGRYGTPQD
                     FMYFVDVMHAKGIGVIMDWVPAHFPRDEHGLARFDGTCLYEHQNPMQSDHPQWGTLIF
                     NYARPEVASFLVSSAMLFFDVYHIDGIRVDAVTSMLYLDYARNPGEFVPNEDGGNIDR
                     SAAAFLQKLNSVILTRYPGVMTVAEESTAYPMITKPPYDGGLGFLFKWNMGFMHDTLD
                     YMSMDPFFRRHHHNKLTFSMHYAFTENFILPYSHDEVVHGKKSLL"
     gene            complement(1315..>1690)
                     /locus_tag="PLZ74_11525"
     CDS             complement(1315..>1690)
                     /locus_tag="PLZ74_11525"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOR98990.1"
                     /translation="GGESIYLPHRYEKNCVVYTGTHDNDTTLGWYQSLGEDERNFLDA
                     YLGGVNEERIHWKLIRLAMSSVADVCIFPMQDVLGLPSSARMNRPQTSQGNWRWRVTE
                     DSLDGELAQKLAGLTKLFGRRR"
BASE COUNT          377 a          498 c          463 g          352 t
ORIGIN      
        1 agcagggatt ttttgccgtg aaccacctcg tcgtgggaat acggcagaat gaagttctcc
       61 gtaaacgcgt aatgcatgga aaaggtcagc ttgttgtgat ggtgccggcg gaagaacgga
      121 tccatggaca tgtagtcaag ggtatcgtgc atgaagccca tgttccactt gaacaaaaag
      181 cccaaccctc cgtcgtaggg cggcttggtg atcatcgggt aggccgtcga ttcctccgcg
      241 acagtcatga cgccggggta ccgcgtcaga atcacggaat tgagcttttg cagaaaagcc
      301 gccgcggagc ggtcgatatt gcccccgtcc tcgttgggaa caaattcgcc cggattgcgg
      361 gcgtagtcca gatagagcat cgacgtgacc gcgtccaccc gaatcccgtc gatgtggtac
      421 acgtcaaaga acaacatcgc gctcgaaacc agaaagcttg ccacctcggg acgggcgtag
      481 ttgaaaatca gcgtgcccca ctgcgggtga tcggattgca tcggattctg atgctcgtag
      541 aggcaggtgc cgtcaaagcg cgccagcccg tgttcgtcgc ggggaaaatg agcgggcacc
      601 cagtccatga tcacgccgat gcctttggcg tgcataacat cgacgaaata cataaagtcc
      661 tgcggcgtcc cgtaacggcc ggtgatcgcg taaaatcccg ttacttggta cccccaggaa
      721 tcgtccagtg gatattcggt gacgggcata atctctacgt gtgtgaaacc catctcgacc
      781 agataatcgg aaagttcatc ggccacgcga cggaaatccg cgaatcgcca atcctccggc
      841 tttcgccatg aaccgatgtg catttcgtaa atggaaacgg gcctgttcag aaggttctgt
      901 ccctgtcgac gcttcaaata ttccgcgtcg tgccagtcgt atttgccgag gctccagacc
      961 tttgaggcgg tcgcgggacg cacctcggca tgaaaggcaa agggatccgc cttgagcacc
     1021 gtttttccgt cgcagccgtg gattgcatac ttgtagcagt ccccatcgcg aaggcccggc
     1081 acgaacgccg caaacacacc cgaatcaagg cgctccatcc ggttgcggga agcgtcccag
     1141 ccgttgaagt ccccgaccac actgacctcc cgcgcattgg gcgcccagac cagaaagcgg
     1201 tgcagaccgg attcattcac gcggtggcag ccgaaagtgg cgtaggcctg ctgcgcctcc
     1261 ccgatattga aaaggtaaat ctcgtcctgt ggaatatcgt ttttgatcat gacttcaccg
     1321 tcgcctgccg aacagttttg tcagaccggc cagtttttgc gcaagctcac cgtccagact
     1381 gtcctccgtc acacgccacc gccagttccc ctgtgacgtc tgcggccggt tcatgcgcgc
     1441 cgaggaagga agtcccagca catcctgcat cggaaaaatg cacacatcag ccacggaact
     1501 catggccagg cgaatcaact tccagtgaat gcgctcttcg ttgacgccgc ccaagtaagc
     1561 atccagaaaa tttcgttcgt cttcgccaag gctttgatac cagccgagtg tcgtgtcgtt
     1621 gtcatgcgtg cccgtgtaaa ccacgcagtt tttctcatac cggtgcggca agtaaatgct
     1681 ttccccgccc
//
LOCUS       DAOSRB010000347         1686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_66486_length_1686_cov_5.063765, whole genome shotgun sequence.
ACCESSION   DAOSRB010000347 DAOSRB010000000
VERSION     DAOSRB010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66486_length_1686_cov_5.063765"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLZ74_11530"
     CDS             <1..253
                     /locus_tag="PLZ74_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98991.1"
                     /translation="VFSNGKTVESLGLDGTETLTIAGLHDLSPRKRLEVVAVKPAGQE
                     IRFEVTARVDNDIEVAWWRNGGILNFVLRQMLGEQPREV"
     gene            complement(250..1110)
                     /locus_tag="PLZ74_11535"
     CDS             complement(250..1110)
                     /locus_tag="PLZ74_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR98992.1"
                     /translation="MANQYDFEYKPDIAWCPGCGDFMIRKALLSALDELNLEREKVVL
                     VSGIGQAAKMPQYVNTSFFNGLHGRGLPPATAIKACNPELTVIAVGGDGDMYGEGGNH
                     FLHVTRRNPDMTLFVHDNMVYGLTKGQGAPTSRPGMETPVQVFGVFEKPVNPMALAIS
                     LDVTFVARVFCGDIEQTKEIMKRAIRHKGFALVDIFQPCVSFNKINTFAWFKENTYYL
                     DDDWNPSNRAASFAKALEEEPFPLGVLYESDHRSTFEENLSVYKKVRTPLYRRSRDPA
                     AVAAEIRAKV"
     gene            complement(1110..>1686)
                     /locus_tag="PLZ74_11540"
     CDS             complement(1110..>1686)
                     /locus_tag="PLZ74_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOR98993.1"
                     /translation="PETPPDRHIMQTDPDYRRFALTGSGISPRGIPGYGEGLVCADSD
                     EHDESGHITESMDMRVRMVEKRKKKHAALLAEAAPPTLSGADNYKTLVICWGSTKPMV
                     AEALEELALPDVAMLHFSQVFPVPTGAAALLEKAEHIVSVENNSSAQFTRLLDAETGI
                     PIRHTILKYDGLPFSVEELAERLSSAVKEGK"
BASE COUNT          346 a          463 c          501 g          376 t
ORIGIN      
        1 ggtgttttcc aacgggaaaa ccgttgaatc gctcggcctc gacggtacgg aaacccttac
       61 catcgccgga ctgcatgatc tctccccacg gaaacgactc gaagtcgttg cggtaaaacc
      121 cgccggacag gaaatccggt tcgaagtgac cgcccgcgtt gacaacgata tcgaggtcgc
      181 ctggtggcgc aacggcggga tactcaactt tgtactgcgg cagatgctgg gagaacaacc
      241 gcgggaagtt taaaccttcg cgcgaatctc cgcggcaacc gcggcgggat cccggcttcg
      301 gcggtacagc ggggtccgga cttttttgta gacagaaagg ttttcctcaa aagtgctccg
      361 gtgatcactc tcatagagta cgccgagcgg gaacggttct tcttcgagag ctttcgcaaa
      421 cgatgccgcg cggtttgacg gattccagtc atcatcaagg tagtatgtat tttccttgaa
      481 ccaggcgaag gtgttgattt tattgaagga tacgcagggc tggaagatat ctacgagggc
      541 gaatccctta tggcggatgg cccgcttcat gatctctttc gtctgttcga tatcaccgca
      601 aaaaacccgg gcgacaaaag tgacgtccag cgagatggcg agcgccatgg ggttcaccgg
      661 tttttcaaag acaccgaaca cctgcacggg agtttccatg ccgggccgcg acgtcggtgc
      721 gccctgcccc ttggttagcc cgtagaccat gttatcgtga acgaacaggg tcatgtcggg
      781 gttacgccgg gtaacgtgca gaaaatggtt gccgccttcg ccgtacatgt cgccgtcgcc
      841 gccgaccgcg atgacggtga gttccgggtt gcatgccttt atggcggtag caggcggcag
      901 gccgcgtccg tggagcccgt tgaagaaact cgtgttcaca tactggggca tctttgccgc
      961 ctggccgatg cccgatacca gcacgacctt ttcgcgttca agattgagct catcgagcgc
     1021 tgagaggagg gctttacgga tcatgaaatc gccgcatccg ggacaccagg cgatatcggg
     1081 tttatattca aagtcatact gattggccat tacttcccct ccttcactgc ggacgaaagg
     1141 cgctccgcaa gttcttccac cgagaacggc aatccgtcgt atttgagtat ggtgtgcctg
     1201 atcggaatac cggtttctgc atcaagaagc cgggtgaact gcgcggagga attgttttcc
     1261 acgctcacga tatgttccgc tttttcaaga agtgccgcag ccccggtggg gacggggaac
     1321 acttgtgaaa agtggagcat tgcgacatcc ggcagggcta gttcctccag tgcttctgct
     1381 accatgggct tcgtggatcc ccaacagatg acgagggttt tgtagttatc cgctcccgaa
     1441 agagtcggag gtgcggcctc cgcaagcagt gcggcgtgct ttttcttgcg cttttcgacc
     1501 atacggacac gcatgtccat ggattcggta atgtggcccg actcgtcgtg ctcatcgctg
     1561 tcggcgcaga cgagtccctc gccgtatccg ggaatacccc gcggggaaat gcccgatccg
     1621 gtgagcgcga aacgccggta atccggatca gtctgcatga tgtgccggtc gggtggcgtc
     1681 tccgga
//
LOCUS       DAOSRB010000348         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_67732_length_1665_cov_5.329814, whole genome shotgun sequence.
ACCESSION   DAOSRB010000348 DAOSRB010000000
VERSION     DAOSRB010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67732_length_1665_cov_5.329814"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /locus_tag="PLZ74_11545"
     CDS             <1..1610
                     /locus_tag="PLZ74_11545"
                     /inference="COORDINATES: protein motif:HMM:NF027658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HOR98994.1"
                     /translation="GLSAEVAFTTPKLTDDLDVFSRPVTYLNVRVAGAKTFAVRTSIT
                     GAFATNDDRAPMTWTTNGVAGATVVAVGRADGTPFSIRGDRQRANWGRACLVGPIADG
                     DTTRYLLAYDNVKSVRFFGQELVDWWRRVGKTFDAMLGEAVRDASALEAKCRAFDVRF
                     RADMEAVGGAKYADIAELAWRQSFAACKFVAGPDKEPFFLSAENASGGMIGTTDVFYP
                     QFPHLLMSSLTLAKASLAPTCIYAASTNWPYAYAPHDLGLFPVAEGQYYGMKKGQSVG
                     GGTDDTYRMPVEECGNMLILLGAVATAEGNAGFAGRWWDEVTKWADYLAAFGFDPGNQ
                     LCTDDFAGHLAHNANLSMKSIVALAAYARMAEMRGETDAAHRFRSLAEGMVPQWRAAA
                     KGGAAGGSRIAFDRPGTWSQKYNLVWDSVLGFNLFPAEVAAAELAAYRAVALPYGVPL
                     DCRKTYTKADWIVWSGTLTGRREDLEFMCEGLHRFLNETPDRIAFGDWYLTDSALYRH
                     FIARSVVGGVFMPVLRHADLVKRYRHP"
BASE COUNT          292 a          514 c          543 g          316 t
ORIGIN      
        1 gaggcctctc ggcggaggtc gcttttacaa caccgaagct gacggatgat ctggacgtct
       61 tctcgcgccc cgtcacctat ctcaacgtcc gcgtcgcggg cgcgaagacg tttgcggtca
      121 ggacgtcgat taccggcgcc ttcgcgacga acgacgaccg tgcgccaatg acatggacaa
      181 cgaatggggt cgccggggcg acggtcgttg ccgtcggccg tgccgacggg acgccattct
      241 cgatccgcgg cgaccgtcag cgcgcgaact ggggacgcgc ctgcctcgtg gggccgatcg
      301 ccgacggtga tacgacgcgc tacctgctgg cttacgacaa cgtgaagagc gtccggttct
      361 tcgggcagga actcgtcgac tggtggcggc gcgtcgggaa gacctttgac gcaatgctcg
      421 gcgaggctgt gcgtgacgcg tccgcgctag aggcgaagtg ccgcgccttc gacgtgcggt
      481 tccgcgcgga catggaagcg gtcggcggcg cgaagtacgc ggacatcgcc gaactcgcgt
      541 ggcgccaaag tttcgccgcg tgcaaattcg tcgcgggccc cgacaaggag ccgttcttcc
      601 tttccgccga gaatgcttcc ggcggcatga tcgggacgac ggatgtcttc tatccgcagt
      661 ttccacatct tctgatgtcg agtttgacgc tcgcgaaagc ctcgctcgcg ccgacatgca
      721 tctatgcggc gtcgacgaac tggccatacg cctatgcccc gcacgatctt ggactgttcc
      781 cggtcgctga aggtcaatac tacggcatga agaaggggca gtccgtcggc ggcggcacgg
      841 acgacaccta ccgcatgccg gtcgaggagt gcggcaatat gctgattctg ctcggagccg
      901 tggcaacggc tgagggcaac gccggtttcg cgggacgctg gtgggacgag gtcacgaagt
      961 gggcagacta tcttgccgcc ttcggcttcg atcccggcaa tcagctttgc acagacgatt
     1021 tcgcggggca tctcgcgcac aacgcgaacc tctctatgaa gtccatcgtc gcgctcgcgg
     1081 catatgcgcg catggcagag atgcgtggcg agacggacgc ggcgcaccgc ttccgttctc
     1141 tcgccgaagg catggtgccg cagtggcgcg ccgcagcgaa aggcggagcg gctggaggct
     1201 cacgcatcgc cttcgaccgt cccggaacct ggagccagaa atataacctc gtgtgggaca
     1261 gcgtgctcgg attcaatctc tttccggcgg aagtcgccgc agcggaactc gcggcctacc
     1321 gtgccgtcgc gcttccctat ggcgtgccgc tcgactgccg caagacgtac acgaaggcgg
     1381 attggattgt ctggtcgggc acgctcacgg ggcgccgcga agacctcgag tttatgtgcg
     1441 aaggcctgca ccggttcctg aacgagacgc ccgaccgtat cgcgttcggc gactggtatc
     1501 tcacggattc agccctttac cgacatttca ttgcccgcag cgtcgtcggc ggcgtgttca
     1561 tgccggttct gcgccatgct gatctggtga aacgctatcg tcatccgtaa gggttcgaat
     1621 cagcggcagg aatgacgttg agacttgacc ataacgtttt cagat
//
LOCUS       DAOSRB010000349         1633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_69766_length_1633_cov_6.294677, whole genome shotgun sequence.
ACCESSION   DAOSRB010000349 DAOSRB010000000
VERSION     DAOSRB010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69766_length_1633_cov_6.294677"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLZ74_11550"
     CDS             complement(<1..286)
                     /locus_tag="PLZ74_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR98995.1"
                     /translation="MKRHLIECGWLAALLCGMVAGAPVAVNGADDSGRAVAKLIEVSR
                     KHPRYFVEGATEKTWIPIGCNICFARLNVSDAEVRANFERWLRRFAANGGN"
     gene            complement(411..>1633)
                     /locus_tag="PLZ74_11555"
     CDS             complement(411..>1633)
                     /locus_tag="PLZ74_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOR98996.1"
                     /translation="YAIVPNQEFWLNAGMKRASAASGIPRVLVTIPTANFTHRKVLEG
                     ILDYARIKGPWQFHLNTGDIAVQDMKRVDNWKCSGIIALSSNKAVLRRLVRFRKPTVF
                     INPPDAGNVPPRGCVFVRRDDRNLGRVAAEYFISGGFRSFAFVGASRPAAWSDDRRDG
                     FTTRLAQEGFTCIDYGSVTPEESADAAIEAPRLLTWLKTLPRQTALFTVKDIRGQQIL
                     SACLDASIRVPEDLAVLSTDNDPVLCDTVSPPLSSISLDGENTGKLCAGILDDLLHGK
                     KVEPVVSIMCPRIITRRSSDVTAVEDPLLARALDYVRAHLDEPFRIGKLASETGISVR
                     TIQLRARKQFGHPFREEIRRLRLAKAISLLSNTDLPAADIAAMCGFCSGSHLSRAVKD
                     AFGYPPSTFRPKPT"
BASE COUNT          345 a          469 c          488 g          331 t
ORIGIN      
        1 agttgccgcc gttcgcggca aagcgccgca gccagcgttc gaagttcgcg cgcacctcgg
       61 cgtccgagac gttgagccgg gcaaagcaga tgttgcagcc gatcggaatc catgttttct
      121 ccgtggcgcc ctcgacgaag tagcgcggat gcttccgcga gacctcgatc agcttcgcaa
      181 ctgcacgccc ggaatcatcg gcgccattca cggccaccgg tgcgcccgca accatcccgc
      241 acagcagcgc cgccagccag ccacattcaa tcaaatgacg tttcattgtt tcttctcgcc
      301 acatgttata ccgcaactcg tggcactgta acacattcca gaaaccggaa gaagtccatt
      361 tcagccaaaa atcagcccta ctgttaccaa accaaagagc tgcggggatg tcatgtcggt
      421 ttcggccgga atgtgcttgg cgggtatccg aaggcgtcct ttacagcgcg tgagagatgg
      481 cttccgctac agaacccgca catcgctgcg atgtcggcgg ccgggagatc tgtgttggaa
      541 agaagcgaaa tggccttggc aagacgcaac cgacgaattt cctcacggaa ggggtgcccg
      601 aactgtttgc gcgccctgag ttggatcgta cgaaccgaaa tccccgtctc gcttgcgagt
      661 ttaccgattc ggaagggttc gtcaagatgg gcgcggacat aatcaagcgc ccgtgcgagg
      721 agcggatctt cgacggcggt tacatcggag gatcggcgtg tgatgatgcg tgggcacatg
      781 atgctgacga caggttcgac ttttttccca tggagcaggt cgtcgaggat tccggcgcat
      841 agttttccgg tattttcgcc atccagggaa atcgacgaca aaggcggcga taccgtgtcg
      901 cagaggactg ggtcgttatc cgtcgagagc acggcgagat cctctggaac gcggattgag
      961 gcgtcgaggc acgcggataa tatctgctgc ccgcggatgt cttttacggt aaaaagtgcg
     1021 gtctgacgcg ggagcgtctt gagccatgtt aaaagccgcg gcgcttcaat ggcggcgtcc
     1081 gcgctctctt ccggcgtgac ggagccgtag tcgatgcagg tgaagccttc ttgtgcgagc
     1141 cgagttgtaa atccgtcccg ccgatcatct gaccaagccg ccggacggct ggcaccgaca
     1201 aaggcgaagg atcggaaacc gccggagatg aagtattccg ccgcgacacg tccgaggttg
     1261 cggtcgtcgc gccggacgaa tacgcagccg cgcggcggga cgttccccgc gtccggcgga
     1321 ttgatgaaaa ccgtcggctt gcggaaccgc acgagccgtc tcagcacagc cttgtttgag
     1381 gacagggcaa tgatgccgga gcacttccag ttatcgaccc gtttcatgtc ctgtaccgcg
     1441 atgtctccgg tattgaggtg aaactgccaa ggccccttga ttcgggcgta gtcgagaatg
     1501 ccctccaaaa ctttccggtg cgtgaaattc gccgtcggaa tggtcaccag aactctgggt
     1561 ataccggatg cagcggaggc ccgtttcata cccgcattca accaaaattc ctgatttggc
     1621 acaatcgcat att
//
LOCUS       DAOSRB010000350         1625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_70282_length_1625_cov_5.956051, whole genome shotgun sequence.
ACCESSION   DAOSRB010000350 DAOSRB010000000
VERSION     DAOSRB010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70282_length_1625_cov_5.956051"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..541
                     /gene="nikR"
                     /locus_tag="PLZ74_11560"
     CDS             95..541
                     /gene="nikR"
                     /locus_tag="PLZ74_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOR98997.1"
                     /translation="MVRYYDRKEKRMGIMRFSVSLEEGLLRRFDAKIRSEQCPTRSKA
                     IADLIRSSLVETEWQAGDEVAGAVVLVYDHHTRNVVKKLTEAQHDCHAAIISTQHVHL
                     DHNNCLEIIAVRGRPDVIKRLVARLKAVKGLKHVSVAAGTTGAQLQ"
     gene            642..>1625
                     /locus_tag="PLZ74_11565"
     CDS             642..>1625
                     /locus_tag="PLZ74_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOR98998.1"
                     /translation="MKKVLVGCAVAVLVGYAQEPLSVTIRADKPGHAIPRTLYGIFFE
                     DINYAADGGLYPELIANRGFDWRTQEPDGWSREWRDGAMGRVSLQGADPVHPNTFQYL
                     RIECYAPGQGAGVANNGFGGIAVQQGRTYALSFYARRHADYAGGLTVQLEDAQRQPLA
                     AFRIAKEAWKTGCPDGRAASPLDSPPPAPWARYEALFEPSATVTNARLVALLDARGTV
                     DLDLVSLFPQDTYKGRKNGLRKDLMEMLAKLKPATLRFPGGCLVEGRSFETQYHWKRT
                     VGPLERRAVNHNLWGYWQSHGLGYYEYFQMAEDLGAEPLPILAAGLTCQFRK"
BASE COUNT          337 a          448 c          542 g          298 t
ORIGIN      
        1 aatggaaccc tgaaacgcat gaccttcgat cgcacccctg tcacgaaccg attactaacg
       61 gtggacgtcc cggggttgcc agcggttggc ggagatggta cggtattatg acagaaagga
      121 aaagcgaatg ggaatcatgc gtttcagcgt ttcattggag gagggactgt tgcgtcggtt
      181 cgacgcgaaa atccggagcg agcaatgccc cacgcgatcc aaagcgatcg ctgacctgat
      241 ccggtccagc ctggtcgaaa ccgaatggca ggccggggat gaggtggcgg gtgccgtggt
      301 gctcgtctac gatcaccaca cgcgcaacgt ggttaaaaaa ctcacggagg cgcaacacga
      361 ctgtcatgcg gcgatcatct cgacacagca tgtgcatctg gaccacaaca actgcctgga
      421 gatcattgcg gtccgagggc gtccggacgt gatcaaacgt ttggtggcgc gactgaaagc
      481 cgtcaaggga ctgaaacacg tatccgttgc ggcggggacc accggcgcgc agctacagtg
      541 agagacaaca ggatcaaagc aaaaacggct tcgtaaaaat gactttgcac ccctgtcgat
      601 ccgtctttga aattggtatg atgccggaaa gggggtgacc cgtgaaaaaa gttttggttg
      661 gttgtgcggt agcggtgttg gtgggatacg ctcaggagcc cctgtccgtc acgatacggg
      721 cagataagcc ggggcatgcg attccgcgga cgctctacgg catctttttc gaggacatca
      781 attacgcggc tgacggcggc ctctacccgg agctgattgc gaaccggggg ttcgactggc
      841 gtacgcagga acctgacgga tggtcgcgtg aatggcgcga cggggcgatg ggaagggttt
      901 cgctgcaggg ggcagaccca gtgcatccca acacctttca gtatctgcgc attgagtgtt
      961 acgcgccggg tcaaggcgcg ggcgtggcca ataacggctt cggcggcatc gcggtgcagc
     1021 agggcagaac gtatgcgctc tccttctacg cgcgtcggca cgccgactat gcgggcgggc
     1081 tcactgtgca actggaagac gcgcagcggc agccgctggc ggcattccgt atcgcgaagg
     1141 aggcttggaa gacgggatgc ccagacgggc gggccgcttc gccgctggac tcgccgccgc
     1201 ctgcgccttg ggcgcgttac gaggcgctct ttgagccgag cgcgacggtg acgaacgcgc
     1261 ggctcgtggc gctgctcgat gcgcgcggca ccgtggatct cgatctggtt tcgctcttcc
     1321 cgcaggacac ctacaagggc cgcaagaacg gccttcgtaa agacctgatg gagatgctgg
     1381 caaagttgaa gcccgcgacc ctgcgcttcc cgggcggctg tctggtggaa ggacgcagct
     1441 ttgagacaca gtatcattgg aaacgcacgg tcggaccgct ggagcgtcgt gcggtgaacc
     1501 acaacctctg ggggtactgg cagagccacg gactggggta ttacgaatac tttcaaatgg
     1561 ctgaggacct cggcgccgaa ccgctgccga tcttggccgc gggcctgacc tgtcagttcc
     1621 gcaag
//
LOCUS       DAOSRB010000351         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_75456_length_1549_cov_8.129183, whole genome shotgun sequence.
ACCESSION   DAOSRB010000351 DAOSRB010000000
VERSION     DAOSRB010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75456_length_1549_cov_8.129183"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1342
                     /locus_tag="PLZ74_11570"
     CDS             155..1342
                     /locus_tag="PLZ74_11570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR98999.1"
                     /translation="MTDITLHPHRDFSVLRRHPWIFSGAVARVSGEPAAGETVRVLGA
                     DGTALGYGAFSPESQIRVRMWTFDVSQTVDEAFIHDRIRRAVGARALFLADDVTDAYR
                     LAHAEADGLPGLVADRYGDWIVCQFSTAGAERWKREIVEALQACVPCRGVYERSDLDV
                     REHEGLAPVTGVLAGEEPPERIQITEYGCRYGVDVRTGHKTGFYLDQRANRHLIKRYA
                     NGAEVLNAFSYTGGFGIAALAAGATRVTHVDLSESALDLARENAALNTCHVDPADFIR
                     GNVFEELRTFRDRGRSFDLVVLDPPKFAETKGSLMKAARGYKDINLLGIKLLRPGGIL
                     ATFSCSGLMTAELFHKVVSDASVDARRDIQVLQRLQQAEDHPEGLCFPEGLYLKGLLC
                     RVW"
BASE COUNT          272 a          407 c          539 g          331 t
ORIGIN      
        1 caaggatgga ctcgtggtca tcttcttttg aagatgcgaa ctcgcttccg gcaatcttga
       61 tccgcgaggc gggagagtgg tatagtatct gataagcgtc cctgcggcag cgatgaaacc
      121 tgagttgacg gggcgtgacg aaagataagg gaagatgaca gacatcacgc ttcacccgca
      181 tcgcgatttt tcagtgttgc ggaggcatcc ttggattttc tcaggcgcgg tggcgcgcgt
      241 atcgggcgag cctgcggcgg gcgagactgt gcgggtgctc ggcgcggacg ggacggcgct
      301 cgggtacggc gccttctcgc cggagtccca aattcgcgtg cgcatgtgga cctttgacgt
      361 cagccagacg gttgatgagg cgttcattca tgaccggatc cgtcgtgctg tgggtgcgcg
      421 cgcgcttttt ctggcggatg acgtgacgga cgcctatcgg ctggcacatg ccgaggcgga
      481 cggtctgccg gggctggtcg cggaccggta cggcgactgg atcgtgtgcc agttctccac
      541 ggcgggggcg gagcgctgga agcgcgagat cgtcgaggcc ctgcaagcgt gcgtcccctg
      601 ccgcggcgtg tacgagcgtt ccgatttgga tgtgcgcgag cacgagggtt tggcgccggt
      661 cacgggcgtt ctggcgggcg aagagccgcc cgagcgtatc caaatcacgg aatacgggtg
      721 ccgttacggc gtggatgtcc gtaccgggca caagactgga ttctatctgg atcagcgcgc
      781 caaccgccat ctgatcaagc ggtacgccaa tggtgcggag gtgctgaacg ccttctcgta
      841 tacgggcggg ttcgggatcg cggcactggc tgcgggcgcg acccgggtga cgcatgtcga
      901 tctttcggag tcggcactgg atctggcccg ggagaacgca gcgctcaaca cctgccatgt
      961 ggatccggcg gatttcatcc gggggaatgt gttcgaagag ctgcgcacgt tccgggaccg
     1021 ggggcggtct ttcgatctgg tggttctgga tccgcccaag ttcgccgaga ccaagggtag
     1081 cctgatgaaa gccgcgcgcg gttataagga catcaacctg ctgggcatca aactgttgcg
     1141 ccccggcggg atcttggcga ccttctcctg ttccggactg atgacggccg agctgttcca
     1201 caaggtggtg tcggatgcct cggttgacgc ccggcgcgat attcaggtgc tgcagcgttt
     1261 gcagcaggcc gaggaccatc ccgaagggct ctgtttcccc gaggggctct acctcaaggg
     1321 cctgctctgc cgggtgtggt agtaacggac gtgccgttct cgataaaaaa agtttcctgc
     1381 cggtgtgacc cggcaggaaa ccgtgtggtg tttatatatt attgggttgt gtgtggagag
     1441 acaatagcac gtttatcccg aaataggcaa tagaaatatt ttgaatatac gcattcggtt
     1501 cgtgacaggg gtgtgcgaag gtcatgcgtt tcagggttcc attgcggcg
//
LOCUS       DAOSRB010000352         1547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_75616_length_1547_cov_7.319705, whole genome shotgun sequence.
ACCESSION   DAOSRB010000352 DAOSRB010000000
VERSION     DAOSRB010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75616_length_1547_cov_7.319705"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLZ74_11575"
     CDS             complement(<1..655)
                     /locus_tag="PLZ74_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR99000.1"
                     /translation="MKANKAMVGIWVLGMALALRADVVREALPDTEARPYVFKAEPNA
                     FAGKPDARAFSFPFDGTRLFADVTNSGTYGALYLTIRATEPDTQAVEVSLKQSDGVTW
                     GLTNLPIGPDWQEIVLPSHLMRPFTHWPSHPRMAPDARPDFRLINEVRFCFGKWLCPD
                     TLAKPHGFEISSIKLVVTEPPSQVAEDKIPRVTFDSDHSLDEFPRYTNETDDSGRFRR
                     "
     gene            816..992
                     /locus_tag="PLZ74_11580"
     CDS             816..992
                     /locus_tag="PLZ74_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR99001.1"
                     /translation="MTENCPPPGVLPLRTLHKSNRLEEFFAYLVRGLRQVDCASGASL
                     VVRALASGKALPCL"
     gene            1154..>1547
                     /locus_tag="PLZ74_11585"
     CDS             1154..>1547
                     /locus_tag="PLZ74_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOR99002.1"
                     /translation="MGLFTILFVLFAWTAVGGTVVTSVSELTRVSAFADREPCAFSLT
                     GRVHAVFSGTSPSSSFVIDDGTGRMPLYASAQTAPVALGDHIVASGKKAVLGWSTDLY
                     DDILTPELDVTVLAHVSRQNIPVPVVGEQ"
BASE COUNT          292 a          528 c          429 g          298 t
ORIGIN      
        1 cacgacggaa gcggccagag tcgtccgtct catttgtata acgcggaaac tcgtcaagcg
       61 agtggtcgga atcaaacgtc acacggggaa tcttatcctc tgccacctgc gaaggtggct
      121 ccgtcaccac gagcttgatc gaactgatct cgaatccatg cggcttcgca agcgtgtccg
      181 ggcacagcca cttgccgaag cagaaccgca cctcgttgat gaggcggaag tcgggacgcg
      241 catcaggtgc catgcgcgga tggctcggcc agtgcgtaaa cgggcgcatc agatggctcg
      301 gcagcacgat ctcctgccag tcgggtccga tcggcaggtt cgtgagcccc catgtaacgc
      361 cgtcgctctg cttgagcgac acctccacag cctgcgtgtc aggctccgtc gcgcgaattg
      421 tcaggtacaa cgcgccgtac gtgcccgagt tcgtgacatc cgcaaagaga cgtgtgccat
      481 cgaacgggaa actgaacgcc cgcgcatccg gcttccccgc aaaggcattc ggctcggcct
      541 tgaacacata cggcctagcc tctgtatccg gcagcgcctc ccgtacaacg tccgcacgca
      601 atgccagtgc cattccaagc acccaaattc caaccatcgc tttatttgcc ttcatgtaac
      661 actcaccttc gaccacagta acctctctca atctttgacc cgtattgtaa cccctctacg
      721 tctccacgtc tacgcgaaaa cagaacttca aatgacccct tcaaataatc ccacaacggc
      781 ggaaatttat aagaacgtcg tcttcggtag ctgaaatgac cgagaactgc ccgccgcccg
      841 gagtgctgcc gctgcgcacc ctccacaagt cgaaccgcct cgaggagttc ttcgcctacc
      901 tcgtcagagg cctgaggcag gtcgactgcg cgagcggcgc gtcgctcgtc gttcgggccc
      961 tcgcgagcgg caaggccttg ccgtgccttt gaggctgatg ggcacgccgc cggagcgttc
     1021 tgtctgccgc acatttccgc cctcctccgc gcgtgcacgc tcccctgagc cttctgagga
     1081 cctggccgca ccggacctgg ccgctcctcg tagcggtgcg gacgaagcga gcggattttg
     1141 atagaatagg gtcatgggcc tcttcacgat tctgtttgtc ctgttcgcgt ggacggcagt
     1201 cggcggcact gtcgtgacca gcgtctcgga actgacgcgg gtgtctgcct tcgcagaccg
     1261 cgagccgtgc gccttctcgc tgaccgggcg cgtccacgcc gtcttttccg gcacgtcgcc
     1321 gtccagctcg ttcgtcatcg acgacgggac gggacgaatg ccgctctacg catcggcgca
     1381 aacggcgccc gtcgcccttg gcgaccacat cgtcgccagc ggcaagaagg ccgtcctcgg
     1441 ctggtcaacc gacctctatg acgacatcct gacgcccgaa ctcgacgtca ccgtcctcgc
     1501 ccacgtttcg cgccagaaca taccagttcc cgtggtaggc gagcagg
//
LOCUS       DAOSRB010000353         1529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_76967_length_1529_cov_4.852103, whole genome shotgun sequence.
ACCESSION   DAOSRB010000353 DAOSRB010000000
VERSION     DAOSRB010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76967_length_1529_cov_4.852103"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1529)
                     /locus_tag="PLZ74_11590"
     CDS             complement(<1..>1529)
                     /locus_tag="PLZ74_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOR99003.1"
                     /translation="HIVDYTVVTDIEVGTVTFNVAVDGLKYGDTETGTIAVTEGGREI
                     AAGTFKPGEACTLKMPAGFGRWSPESPRLYDFTAKFGSDAVNGYFAMRTFEKRRDAKG
                     VWRFYLNNEPYYVMGTLDQGWWPDGLLTPPSDEAMAFDIQTLKDCGFNTMRKHIKVEP
                     LRYYYLCDKLGLLVLQDMPCNSVDARGPFDWQTAKGYGLYREELKGMMDLLQKVPSIV
                     MWVPYNEGWGQHDQNLTHTTLDFVKKHDPTRLVNGPSGWADFEGGEIYNDKGQAPRRT
                     RTAHRPAGECEAADAVDYHYYRGPKMPPLNDRRVSFLGEFGGLGHPVEGHIWRTFDQA
                     ASLGGEKSGDWGYGGIEDTKTREGLEKTYLGLMEELGKLAGWGLAGSIYTQTTDVEIE
                     INGLLTYDRKVLKYNPAVLKQAHAAVAAKAKASALNAPGNGTDLLYISHVHRGGGKLE
                     RPDNTLETFEWCWENGSALECDCRKTKDGVGIMLHDNTLARTVRGIPEEMKSKSVSND
                     L"
BASE COUNT          236 a          519 c          456 g          318 t
ORIGIN      
        1 cagatcattc gagacggatt tcgacttcat ctcttccggg atgccccgca ccgtgcgcgc
       61 gagcgtgttg tcgtggagca tgatgcccac gccgtccttg gtcttgcggc aatcgcactc
      121 gagggcactg ccgttttccc agcaccactc gaacgtctcg agggtgttgt ccggacgctc
      181 cagcttcccc ccgccgcggt gcacgtgcga gatgtagaga aggtccgtgc cgttgccggg
      241 ggcgttcagc gccgacgcct tggctttggc ggccaccgcc gcatgcgcct gcttgagtac
      301 cgcgggattg tacttgagca ccttgcggtc ataggtgagc agtccgttga tctcgatctc
      361 cacgtccgtc gtctgggtgt agatggaccc ggcgagcccc catccggcaa gtttgccgag
      421 ctcctccatc aggcccaggt atgttttctc aaggccctcg cgcgtcttcg tgtcttcaat
      481 gccgccgtag ccccagtcgc cgctcttttc tccaccgagc gacgccgcct ggtcgaacgt
      541 gcgccaaatg tggccttcga ccggatgccc gagcccgccg aattcgccga ggaacgaaac
      601 gcgccggtcg ttgagcggcg gcatcttcgg gccgcggtaa taatggtagt ccaccgcgtc
      661 cgcagcctcg cactcgcctg ccggcctgtg ggccgtccgg gtgcggcgcg gggcctggcc
      721 tttgtcgttg tagatttcgc cgccttcaaa atccgcccag ccggacgggc cgttcaccaa
      781 ccgcgtggga tcgtgtttct tcacgaagtc gagcgtcgtg tgcgtcaggt tctgatcatg
      841 ctggccccac ccctcgttat agggaaccca catcacgatc gacggcacct tctgcaggag
      901 gtccatcatg cccttcagct cttcgcggta gagaccgtag cccttcgccg tctgccagtc
      961 gaacgggccg cgcgcgtcga cactgttgca cggcatgtcc tgcaggacga ggagaccgag
     1021 cttgtcgcac aggtagtaat agcgaagcgg ctcgactttg atgtgcttgc gcatcgtgtt
     1081 gaacccgcag tccttcagcg tctggatgtc gaacgccatc gcctcgtcag aaggcggcgt
     1141 gaggagaccg tccggccacc acccttggtc gagcgtgccc atcacgtagt agggctcgtt
     1201 gttgagatag aacctccaca cgcccttcgc gtcccggcgc ttctcgaacg tacgcatggc
     1261 gaaatagccg ttgaccgcgt cgctcccgaa cttcgccgtg aagtcgtaga gccgcggcga
     1321 ctcggggctc caccgtccga agccggcggg catcttgagc gtgcacgcct cacccggctt
     1381 gaacgttccc gccgcgatct ccctgccgcc ttccgtcacc gcgatggtac cggtctccgt
     1441 atcgccgtac ttgagcccgt cgaccgcgac gttgaacgtc accgtgccga cttcaatgtc
     1501 cgtcacgacc gtgtagtcga cgatatgcc
//
LOCUS       DAOSRB010000354         1524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2278
            NODE_77343_length_1524_cov_6.236215, whole genome shotgun sequence.
ACCESSION   DAOSRB010000354 DAOSRB010000000
VERSION     DAOSRB010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864345
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:05:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,320
            CDSs (total)                      :: 2,283
            Genes (coding)                    :: 2,270
            CDSs (with protein)               :: 2,270
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77343_length_1524_cov_6.236215"
                     /isolate="AMR_MDS_2278"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PLZ74_11595"
     CDS             complement(<1..1106)
                     /locus_tag="PLZ74_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR99004.1"
                     /translation="MKHRLKKTSRREFLGTLAVGAAASVLENGCRSACLSGWWTEPIE
                     PSSSKDFCFTAPFDGGIIHEQSGTPVLGFETGADGTRMLKVEVSGVDPSGVTPELCTT
                     DGRTIPVTLQNGAFRGTALLRDRVTDIRARARIGGERRVIRTRPVWVKNSTPRFRCYI
                     DDHSFFFRDINRQRYKSIFDCFYLGKLRDLNRNFGVKINLNCFNTTPERDFSLSMFPD
                     TYKSEFEDNAHWLRLAFHSENEFPDIPYLNATPEKLAADFDLVAGELKRIAGSAYTAG
                     LQIHWADVPPDCYKVLADRGVKMLPTRGRKPDAKEKKICDYHLPDPVLEYLYYQQGWM
                     HFESGLIFYSSGTGTREWIPVDKTVPGLLKHIENP"
     gene            complement(1158..>1524)
                     /locus_tag="PLZ74_11600"
     CDS             complement(1158..>1524)
                     /locus_tag="PLZ74_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR99005.1"
                     /translation="GTVKCVAGTTDAASRFSGVGTLAGSFGILTLDVDAEATDGLTFA
                     DLTATQVVADFGRERDDPVPSGGSAVVAKIGAGASFTGMAWRAKNLGADRAADFVCDA
                     NGVVTAHFRNIAGLIIYMR"
BASE COUNT          289 a          479 c          467 g          289 t
ORIGIN      
        1 ggattctcaa tgtgcttgag gaggccggga acggtcttgt ccaccggaat ccactcgcgc
       61 gtgccggttc cggaactgta gaagatcagg ccgctctcga aatgcatcca cccctgctgg
      121 tagtacagat actcgaggac cgggtccggc aggtgataat cgcagatctt cttttccttc
      181 gcgtcaggct tgcggcccct tgtcgggagc atcttgaccc cgcggtcggc cagaacttta
      241 taacagtccg gcgggacatc cgcccagtgg atctgcaggc cggccgtgta ggccgagccc
      301 gcgatccgct tcagctcccc ggcgacaaga tcgaaatcgg cggccagctt ttcgggcgtc
      361 gcgttcaggt acgggatgtc cgggaactcg ttctcggaat gaaacgccag ccggagccag
      421 tgcgcgttgt cctcgaattc ggacttgtag gtgtccggga acatggagag gctgaagtcg
      481 cgctccggcg tcgtgttgaa gcagttcagg ttgatcttga cgccgaagtt ccggttcagg
      541 tcgcggagtt tcccaaggta gaagcaatcg aagatgctct tgtaccgctg gcggttgatg
      601 tcccggaaaa agaagctgtg gtcgtcaatg tagcagcgga aacgcggcgt ggagtttttc
      661 acccacacag gccgggtgcg gataactcgg cgttccccgc cgatccgggc ccgggcccgg
      721 atatccgtca cccggtcccg gagcagtgcg gtgccgcgga aggcaccgtt ctggagcgtc
      781 acgggaatgg tccggccgtc cgtggtgcag agttcgggcg tcacacccga tggatccaca
      841 cccgacacct cgactttcag catgcgcgtc ccgtccgctc cggtttcaaa ccccaagaca
      901 ggcgtgccgc tctgttcgtg aatgatgccg ccgtcaaacg gcgcggtgaa acaaaaatct
      961 tttgacgagg acggctcgat gggctccgtc caccagcccg acagacaggc cgaccggcaa
     1021 ccgttttcta acacgctggc cgctgcgcca accgccagag tcccaagaaa ttcccgccgc
     1081 gatgtttttt ttaatcggtg tttcataagt ctcccccggg gggtgatgca ggaggcgata
     1141 gaaacggggt cgatccgtca gcgcatgtaa attatgaggc cggcgatgtt acggaagtgc
     1201 gccgtcacaa caccgttcgc gtcgcatacg aaatccgccg ccctgtcggc accgagattc
     1261 ttcgcccgcc acgccatccc cgtgaacgag gctcccgcgc cgatcttcgc gacaacggcg
     1321 ctgccgcccg acgggacagg atcatcgcgc tcgcgcccga agtcggcaac cacctgcgtg
     1381 gccgtgaggt ccgcgaacgt gaggccgtcc gtcgcttccg catcgacatc gagcgtcaag
     1441 atgccgaacg accctgcgag cgtacccacg ccggagaaac ggctggcggc atctgtcgtg
     1501 cccgcaacac acttgactgt acct
//
